LOCUS       DAMJYR010000001        91147 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.001, whole genome shotgun sequence.
ACCESSION   DAMJYR010000001 DAMJYR010000000
VERSION     DAMJYR010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 91147)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 91147)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..91147
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.001"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            274..1212
                     /gene="pdaA"
                     /locus_tag="PC471_000001"
     CDS             274..1212
                     /gene="pdaA"
                     /locus_tag="PC471_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="delta-lactam-biosynthetic de-N-acetylase"
                     /protein_id="HDF2787153.1"
                     /translation="MFRKVLHYLTLLSISIFFIVGCSNSQNNQNENQNKETQLQEDKE
                     KIDSGKDTSNVIVSDGTDKPSKATTNNDNNKLDVSSLDNTTLDWFYIPNNKHKTPEVN
                     TDIEFKFSDYDALYNGPTKDGQKTLYLTFDEGYENGYTTKILDTLKQNQVKAVFFVTA
                     PYIKENKDLVKRMVSEGHIVGNHSKTHPSMPTKTSNLKNFNDELYDVEKLYKDVTGKD
                     MVKFFRPPMGKYSEKSLAMTKNLGYKTVFWSFAYRDWDTDKQPSHEEATQKIMDNLHD
                     GSILLLHAVSKTSTEILNDFISNARKLGYEFELLEY"
     gene            complement(1584..2957)
                     /locus_tag="PC471_000002"
     CDS             complement(1584..2957)
                     /locus_tag="PC471_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2787154.1"
                     /translation="MVLTGAHYIYILFMVIILITMIMKKDTIVPCILGVFFMGLFFEK
                     NIFGAITAVFNSFIISLNELGPIILIIAIMVALSKALEANNAIQYMVRPFSRVIKNSN
                     TAFFVTGFVMLVLSWFFWPTPAVALVGAVFLPVAMRAGLPAIGVAVALNLFGHGLALS
                     TDFVIQGAPSITAGAAGVAVSYVINDGMILFWVMGIVTISMAFYTLKRDINKGMFREE
                     LKGFESEEVEEFSGKSKIATILVALGFLADIIAMYIFDLKGGDASALLGGTAVFLIII
                     INVINFGKDSLENVCENIIDGFVFGIKIFGAIIPIAAFFYMGEVAPLTGVFGKVLAPG
                     SQGLLSDIGIALSQTIPLNKFAVSGIETVVGAITGLDGSGFSGISLVGSLASVFGTAI
                     NASVGALAALGQISGIWVGGGCLVPWGLISAAAICGVSPIELAKRNFVPVITGLVVTT
                     IVAMFII"
     gene            complement(3144..4466)
                     /locus_tag="PC471_000003"
     CDS             complement(3144..4466)
                     /locus_tag="PC471_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF2787155.1"
                     /translation="MINKIKPSKLGVILLIYVVISAAVAFILNIPFYSAVESIVKDVS
                     EGKVYFKREVNQLMDNFQTYVNNNGIRADDGDDIAKWNSDNWFVSMKIYKGNKVFYDT
                     LYYPNEVPDKSEENEFYSSPMARNYEIQFSDIKANIVVVALFRFRFQNFIEAISITFM
                     ALTFIITFLLLFSRKIKYLRKIEMGIKIVESGSLKYRIPVKGNDELSSLASSINEMSH
                     TLENQLIKEREMKNKNKDIVTSISHDVRTPLTSVICYLDLIKDHKYNSPEQLEQYIKN
                     VRSKAYQIKDLTDDLFTHFVNSGEEVSKGYEKINANEFIFQLLLDSSYTLEEKGFHIT
                     IENELYNQYHIYVDVTQFRRIFDNLCSNIIKYARKDKPIVFLIKVEDSKLLIIQKNRV
                     SNFSKNVESFGIGIKNCEKIVAYHNGSINVNSDDKEFAIEISIPAKES"
     gene            complement(4459..5163)
                     /locus_tag="PC471_000004"
     CDS             complement(4459..5163)
                     /locus_tag="PC471_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2787156.1"
                     /translation="MNKEIPKKILVADDNPEIREIVEILLTGEGYEVIMATNGEEAVN
                     LVDSTIDLIILDVVMPVKTGFVACNEIREKTTAPILFLTAKTQDSDKVIGFSAGGDDY
                     LSKPFSYSELISRVKSLLRRYYIYQGKEKEREPNILKTKELCVNLDTQDVTLNGEPVF
                     LTTIEYSILILMLKNRKKVFSSENLYETIWDEQYFYTANNTIMVHIRNLRKKLEKDQK
                     NPQYIKTAWGKGYYID"
     gene            complement(5346..6410)
                     /locus_tag="PC471_000005"
     CDS             complement(5346..6410)
                     /locus_tag="PC471_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HDF2787157.1"
                     /translation="MISKTEKKNKCLGSVAFLVLLMGITGYFVFRGQSVESLIKSLKG
                     ASPMFILIGFAMMFIYVACEGINIYLGMKALNQKTTLLKCMGYAFIGFYFSSITPSAS
                     GGQPAQVYYMKKDDINISYSSLILLVIVVIHQVVILAYSGIMFIMEREFILNNVSGMN
                     ILLIYGVITNVALVIGVIAIIFSKKLVNNFIISITNLLGKLRIIKDVESSRKVIISQI
                     EEYVKGAQYIKQNPKLVVQILVITIVQITAMFLVPFFVYKAFHLSTYTVFEILAIQSL
                     LNIAVSSLPLPGAVGASENSFMTLFKIFFPGHLLVPAMLLSRGISFYAFVAISGLICI
                     VVHVKSSEKVKAVKKVAYVR"
     gene            complement(6413..7111)
                     /locus_tag="PC471_000006"
     CDS             complement(6413..7111)
                     /locus_tag="PC471_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HDF2787158.1"
                     /translation="MYVVGLIIVVALIFLVHSIIPTYYNKLLNKEVLKNMAGENEIAL
                     TFDDGPDKRYTEKLLDVLKENDIQAMFFVVAKNAEKEPEIIKRMLRENHIVGLHSLEH
                     RNAWLYSYSYVKKDFIESTNIMKNLGVDVNYYRPPWGHTNIFSNSFVKKYNLKMTLWD
                     VMAEDWEKDSTVDIIINKLMSRTKENSIICLHDAGENSGGAVGAPERTIEALKIAIPK
                     LKASGLKFVTPERM"
     gene            complement(7102..8199)
                     /locus_tag="PC471_000007"
     CDS             complement(7102..8199)
                     /locus_tag="PC471_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-diphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HDF2787159.1"
                     /translation="MKVLILTGKFGMGHYSASNSLSEDIKAKFDNSEIIIKDIFEYIM
                     PNYSDKMYKTFSILVNRGSSLYNLFYKCAENGKKDIKFTFSDYFLNKLDTLLHEVQPT
                     VVISTFPFCSQLVSRYKEKYNSNLPLITCITDISSHSEWISKNTDCYLVASKSTKEEL
                     VFKGIDESKIKVNGIPVKKEFKRIEHVNHSTKKNILIMGGGLGLLPKSEQFYKELNSL
                     EGVKTTVITGNNKKMYYKLYGRYENIEVVGYTNEVYKYMKDSDLIISKPGGITLFETI
                     YSELPILAFNPFLQQEIDNASFILNNEIGRILGKNKKYYVDEIKDLIYDDATLKEMSS
                     NMKELKKQFDNNTLENILFSLDEQGACRECM"
     gene            complement(8522..9766)
                     /locus_tag="PC471_000008"
     CDS             complement(8522..9766)
                     /locus_tag="PC471_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HDF2787160.1"
                     /translation="MFENLKKADPEIYESMKRELKRQQRNIELIASENIVSVPVMETM
                     GSHLTNKYAEGYSGKRYYGGCEYIDEVETLAIDRIKKIFGAEHANVQPHSGANANIGV
                     YFAMLKPGDVVMGMNLSQGGHLTHGAPVNISGQYYKFYEYGIDKDSGLIDFDEVRKIA
                     HEVKPKMIVAGASAYPREIDFKKFREIADEVGALLMVDMAHIAGLVAAGLHQNPCEVA
                     DFVTTTTHKTLRGPRGGVILCKEKYAKDIDKAIFPGIQGGPLEHIIASKAVCFKEALS
                     DEFKEYQVQVAKNAKALAEELIKRDFKLISGGTDNHLILLDLTNKNITGKAAEKRLDD
                     AYITANKNTIPFDPNGALITSGIRLGTPAVTTRGMKEEDMAIIAEAIDLCLTYDEESK
                     ARTLVVGLTEKYPLYEEYNIMD"
     gene            10094..10882
                     /locus_tag="PC471_000009"
     CDS             10094..10882
                     /locus_tag="PC471_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HDF2787161.1"
                     /translation="MNQNEELEYKKNILKLALFVGELMIKNGAETSRVEDSVLRICRS
                     RGFYHVNIFTTPTVIIISDEKFDGFSFMKTIQSRGINLNKISLLNSFSREFVSDKEYD
                     IDNAIARLYEIQDVKPYPLWLFYSCTGIASACFACLLGGNYVLNFILTFIIAIFAAIT
                     YDKTMKISAISAFSCLVSSFLIALSGVLLVEIGILDDPKMLIVGAIMPLLPGVAFIKA
                     IRDLISGNLISGISRAFDAAMIIIAIASGVGFVLDTWYRIGGPF"
     gene            10882..11355
                     /locus_tag="PC471_000010"
     CDS             10882..11355
                     /locus_tag="PC471_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HDF2787162.1"
                     /translation="MTDMSMILHFLFSGVATAGFAVFFNAPLYLLLPAGVTGGIGWIV
                     YVYLFNFTTNAVFAGFIAAALVSACSETLARKLKQPAIVFVIPGILPLIPGIGLYNTM
                     LSLIQKNYSLAMSKGTDALFLSAAIALGVLVVTSFVRTLNLLKIRKNFIPYRKSK"
     gene            complement(11447..11887)
                     /locus_tag="PC471_000011"
     CDS             complement(11447..11887)
                     /locus_tag="PC471_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR reducing system RseC family protein"
                     /protein_id="HDF2787163.1"
                     /translation="MNQQGFIMEIVDDRTAKLKMQRHSACAACGKCATTNSAESKEIL
                     VEVDNTIGAKVGDHVEVSMDNMNVLKAAVMAYIIPLIALLLGTIGTYYILGAIGMTTG
                     VEAISGLVGICLTIICYLYLKKNDKKFRDSREFIPVITRILIDL"
     gene            complement(11902..12195)
                     /locus_tag="PC471_000012"
     CDS             complement(11902..12195)
                     /locus_tag="PC471_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HDF2787164.1"
                     /translation="MEERKLAPNNEREALIKRLKRIEGQVKGIQKMVEQERYCVDILV
                     QISAIRSAINRVGTIILENHIKGCVAESIKHDDADKTEEMIKELMDTINKFTK"
     gene            complement(12238..12639)
                     /locus_tag="PC471_000013"
     CDS             complement(12238..12639)
                     /locus_tag="PC471_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2787165.1"
                     /translation="MKKCLYCNRDIKSPNNKLAIKYDDNMNIYPCRAECKEKIEEYIA
                     KKPTYKFINILEVLLGVGGIFCFLSKWTIAAQVVLGIDALVIFLFPLAGFKMNGKLSM
                     EQTKNQSRSIAISILAFIVIITVLYFKQVGK"
     gene            12872..14992
                     /locus_tag="PC471_000014"
     CDS             12872..14992
                     /locus_tag="PC471_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDF2787166.1"
                     /translation="MKQMELAIVSLKKDAGEIYENQIRQFLGDNLKINLYSFEEGNLK
                     FFKEKLILLSAYLKYDEIVKLSHYDAQIIVPKLTFEKNSIDMISKLEKDKIIYVYNLS
                     KDMAIETISLIHRLGIDNINILPCYPEIEFTPTDAVILTPGEKILPKFKNCEVIDLKY
                     RIIDLSCIVEIATKTKLKHLIKDDLIKKYVEKIIPTSYSTGELLLDANKFERQFDLLL
                     SIIDDGIICTNNDGIIQFYNHMARKILSINANEMIDSFVGDCIKDINFQSILTNKTPF
                     FEKLIKINHIDINLEIKHIQLNVFDGFILKMTKFSQLEKKQAKLRAQLVNSGNISKYT
                     FDDILGSSIQTINTKKIANKMAQSNSSILIIGESGTGKELFAQSIHSASRRKDGPFVA
                     VNCSTFQENLLQSELFGYDEGAFTGAKKGGKIGLFELANNGTIFLDEIGEMDLNSQSK
                     LLRVIQEKQVRRIGSNNVIDIDVRIIAATNRNLKELVSKNMFRRDLYFRLNVLPLKIH
                     PLRERPADIFEIFGSLKYDIPCNFILSEEVKEIFKMYRWEGNVRELRNLGEYFCYLEK
                     DIIEICDLPEYILDTIDSNYSRTVCNKVSDNIKKYQFNIGKDKNIMKYDYNFKRSLDE
                     YIFILDNLKKAYDLKERIGRKSLCKIALEENRFLTEQQIRNMLLELQNFGLVDILVGR
                     GGSIITSKGVEFLKNINRSNKLNS"
     gene            15217..16383
                     /locus_tag="PC471_000015"
     CDS             15217..16383
                     /locus_tag="PC471_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HDF2787167.1"
                     /translation="MNYNFNEIVDRSNNFSSKWSEMEKKYGTNDLLPMWVADMDFKAA
                     PCIIDSLKNRLEQEIYGYTTRPDSYNESIVNWLDRRHNWKIKSEWLIYSPGVIPAISL
                     LINELTKANDKIMIQEPVYSPFNSVVKNNNRELIISPLQKLENGNYIMDYEDIENKIK
                     DVKLFILCNPHNPVGRVWTKDELKKLGDICLKHNVKIISDEIHSDIILKKHKHIPMAS
                     ISKEFEKNTITCMAPTKTFNIAGLQSSYVILPDEKDYKLLDDAFTRIDIKRNNCFSLV
                     ATEASYNNGESWLESFLEYLESNIDFAIKYINENMPKLKVRKPEGTYLLWVDFSALGL
                     SDEELESILVQKGKVALNQGNSFGIGGSGYQRINLACPRSMLEEALIRIKNAIN"
     gene            16408..17850
                     /gene="nhaC"
                     /locus_tag="PC471_000016"
     CDS             16408..17850
                     /gene="nhaC"
                     /locus_tag="PC471_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HDF2787168.1"
                     /translation="MKMNTERKIPSFRYSLLVMLAIIALTSVGIVVFNASITTMFLLS
                     WLIVVPAAMKLGYTNDEIEAFGFEVGKDAFQSNLIILSVGVLIAAWIAAGTIPTVVYS
                     GLTIITPKYFLLTTLIVCSLTSVATGTSWGTLGTSGIAMMSIGTSMGIPLGLTAGAVI
                     SGAFFGDKMSPLSDSTNLAAAVCKTDVITHMKHMFYTTGPAYVICIVLYTVIGFKYSN
                     NTIDYVQINQIKDVLNSNFHIGLVAMIPIIFLLLLLLLQKPPIISILSSAIMGLIIAV
                     FQEGEKIGDVLNYMLSGFTIDTGFVYADKLLNRGGIMSMAETVLLVFVVFVIAGILQK
                     TGFLEVLLQPLITKIGKSRTKLVGSTFIVSYFANAFSSSMMFTSVFVGTLMSPIYKEF
                     KLKPQNLSRIIEDTATLGGPLIPWNSNAVFCAQTLGVSPLEFIPYCFLSWITPIISFI
                     YGVTGFTMLTYTDDEIEELEIFDTAQRRVD"
     gene            17853..18122
                     /locus_tag="PC471_000017"
     CDS             17853..18122
                     /locus_tag="PC471_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2787169.1"
                     /translation="MKFEYYYLIQDIAGILLAFIGLRMSIIGFRILSMKGLSINTLLI
                     VIKYCLFTIAGLNLLISKFGIRHWIWSVCMLIISIIINPRIKVSK"
     gene            complement(18199..19626)
                     /locus_tag="PC471_000018"
     CDS             complement(18199..19626)
                     /locus_tag="PC471_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp14"
                     /protein_id="HDF2787170.1"
                     /translation="MKLTKSISSMLCLSLVLSLSPVAYANEISRTTVENTLVGQDRYQ
                     TAVEVSKKGWSSANEAVVVNSESIVDALAVTPFAKLKNAPILLTEKNNLNSQTEKELK
                     RLGVKKVYIIGLSSSVSNDVQKQLESNNLTTDRVGGSDRYQTALSIAKKIEGLKDISE
                     IAVVNGYTGLADAVSIASVAATNGMVILPVSDASGISSFKDFIESKNINKSYIIGSTN
                     AVSDKIKQSLPNSERIGGADRNETNGKVIEKFYTSNKLNNVFVAKDGMKNQNQLIDAL
                     AVGVLASKENSPVLIVGEQLSATQKSVFSNKNATSITQVGSGGNENAFKELKDLQANS
                     NNKRVMYVTNTDKVNIRNDATIEASVIGYLNNGDEVEVLDVLKTGWVEIKYNEGIGYV
                     SGSYLTNNKPDNSNENIKIKYVKEKDGLNVRKGPSTEDEKIGHLSYGSKVETIEMFAT
                     GWVKIKYNGGYGYVSNDYLSDTPVI"
     gene            19998..20867
                     /locus_tag="PC471_000019"
     CDS             19998..20867
                     /locus_tag="PC471_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDF2787171.1"
                     /translation="MDLLHLKYFQTVARMEHITKASHKLNIAQPALSKTISSLEKELG
                     VQLFDRKGRYIVLNEYGRLFLKRVDSILDLVESSKKELQDTSLENSGEVKILSPAAAN
                     VLPSLLSNFRKLYPNITFNVSHTLPSSYKKSDFDLYISSSFTKLNSENSITLTCEEIL
                     LGVSINHPLSLKDEVYLSEVSDENFIVITKGENYREVIDILCESANFKPKIAFESDSP
                     YTIYALIKSLQGVGFICGKSWGLSQDPEIKLLHIKDIEFKRYLNLSWFSENYESKAVL
                     LFKNFLINYFKNI"
     gene            complement(20979..21785)
                     /locus_tag="PC471_000020"
     CDS             complement(20979..21785)
                     /locus_tag="PC471_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase"
                     /protein_id="HDF2787172.1"
                     /translation="MGRKVKIGIIQQHSVLGNVKKNIEKAVEMIDDLGKQGADIICLP
                     ELFATGYNLESLGGVKTLELIREHNKYIEESMSEAAKRNNVYLISPYGTLEKGSTHVY
                     NSAVIFDRKGKIMGEYCKNHLWSLEAVYFKGGEKVEVYDADFGRFGVMICYDAGFPEV
                     SRELTLKGSEIIFIPSAWRIQDEDMWDLNVSQRALENTVYTVGVNLVSNDSNLILFGK
                     SKICNPRGTVITQLDTYREKYVLSEIDLDEIKKYREDIPYLKDRKSSYNI"
     gene            complement(21799..23031)
                     /locus_tag="PC471_000021"
     CDS             complement(21799..23031)
                     /locus_tag="PC471_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HDF2787173.1"
                     /translation="MQEREELVLNPVPKEERVGWLAPLFNMLGSNIAISELMVGGTLI
                     LGMTLSNMIITSIIGNLILVAIIMIQGYIGYKEGLNTYVLAKGAFGEIGGKYLISLLL
                     GITSFGWFGVQAGVAGLSVQKIFPSVNLTLVTVILGLFMVVFALYGFKAMAKFNYLVI
                     PPLMILMVWGVFKAFSTYGVEAIYNYTPQTTMSMVEGLNIVVGLVIVGAIISPDQLRY
                     TRRVKDIWIISFLGLGIISLFQQVAAGVMSMGAPTWDITEVLANLGFGWVAFVILILA
                     SWSTNVSNAYSGGLALKTIFPNVKRNVLTLVAGLIGTIIAATGIIFKFQSFLSFLGIA
                     VPAIAGIMWCEYYFIQGRTYKHREGINWIAVISWLIGFAASYYSSKINFLIPPINGIV
                     VSMVIYYILMNCFGIKDK"
     gene            complement(23490..25277)
                     /gene="aspS"
                     /locus_tag="PC471_000022"
     CDS             complement(23490..25277)
                     /gene="aspS"
                     /locus_tag="PC471_000022"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HDF2787174.1"
                     /translation="METLKGLKRTHYCGELREKNINEEVVLMGWVQKKRNLGGLVFVD
                     LRDTSGLCQIVFDTDVDKEAFEKAEKLGAEFVIAVKGKVCERQSKNPNMPTGDIEIFA
                     TELRLLNKSETPPIYIKDDDDVSEALRLKYRYLDLRKPSMQRNLKLRHKVMNITRNYL
                     SNNRFCEIETPFLIAPTPEGARDYLVPSRVNPGKFYALPQSPQLYKQLLMVSGMDRYF
                     QIVKCFRDEDLRADRQPEFTQIDCEMSFVEQEDVMSMIEGLLEAIFKEVLDVELALPL
                     PKMTYAEAMSKYGSDKPDTRFGYELTDISDVVCNCGFKVFADATQPGKSVRGINVKGK
                     ADDFTRKQISSLEEHAKTYRAKGLAWMKVGQEGVTSPIAKFFNEEEMNAILTRMNAEV
                     GDLLLFVADKNSIVFDALGQVRLEVANRLNLLDKNVYNLLWVTEFPVFEEDEETGTFS
                     AMHHPFTSPMDEDLDKLEEGDKSSLRAKAYDIVLNGYEIGGGSVRISNSDVQSRMFKA
                     LGFTEERANEKFGYLLEAFKYGTPPHAGLAFGLDRLVMLLAGTDNIREVIAFPKNQNA
                     VCPMTNAPTLAEDEQLEELSIKVDIKDNE"
     gene            complement(25351..26613)
                     /locus_tag="PC471_000023"
     CDS             complement(25351..26613)
                     /locus_tag="PC471_000023"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HDF2787175.1"
                     /translation="MLTKAPRGTKDITPKEAYKWRYVENKFREICALYGYEEMVTPIF
                     EHTELFKRSVGDTTDIVQKEMYSFKDKGDREITLKPEGTAGVVRAFIENKLYADTQPT
                     KLFYVTPCFRYERPQAGRQRQFHQFGIEALGSDTPSMDAEIIALAVQFFNEVGLNDLV
                     VSINSVGCPVCRKEYNALLKEYLDSKADILCDTCNERREKNPMRVIDCKNPTCKENIK
                     DIPFIADHLCDDCKSHFDKLQEYLKEMNINFVIDKTIVRGLDYYRKTAFEIISNDIGA
                     QSTVCGGGRYDGLVEQLGGPKGISGIGFGLGIERLLLTLEGNGIEIENPQSTDIFIVT
                     IGEEANTRSFKLLKDLRQNHISADKDHIERSVKAQFKYSDKINSKFTIVIGDDELKND
                     TATLKNMKTSEQTTVKLSTLVEELKQKL"
     gene            complement(26603..28159)
                     /locus_tag="PC471_000024"
     CDS             complement(26603..28159)
                     /locus_tag="PC471_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coproporphyrinogen III oxidase"
                     /protein_id="HDF2787176.1"
                     /translation="MLYVFLKGHDYKYEVAELIKLFTSEFKFKDSNANNNIESKHLIN
                     RLIYENGVLFSSTEYYENGNLKYESIQNIENMNLEFLDGFDSSKTIEEFLNSLDSESK
                     RNFKKLCKENIKKSMFLVLKQVFNSYVPWGILTGIRPVKIVHNLMDKKLDDNSIRTIL
                     KDNYFIIDEKIDLALEIAKRERLFMYPIDKNKISLYVSIPFCPTRCVYCSFPSNSLKQ
                     FGHLKREYVYKLIEEIKGFAKVIKDTKKEVETLYIGGGTPTTLDEEELDLLINSLFNE
                     LDLSKIKEFTVEAGRPDTITEQKLRVLKKHNVSRISINPQTMNDDTLVKIGREHRVSD
                     LVDCFNMARRMGFDNINMDIILGLIDEDLNMVKNTLEEIKKLSPESLTVHTLAIKRAS
                     NLNINMDRYKEHLTQYEEMVKMIDLSMKYAKDMGLNPYYMYRQKHMLGNLENIGYAKE
                     GYECIYNIQIMEEKQSNYALGAGAISKFVYVDEDRIERVENVKNVEQYIERVDEMIKR
                     KKEEVYKNVD"
     gene            complement(28250..28864)
                     /locus_tag="PC471_000025"
     CDS             complement(28250..28864)
                     /locus_tag="PC471_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDF2787177.1"
                     /translation="MIIKKFVDSYFGVNTYVLGDEKTKKCAVIDPGGSLVETLSFVKE
                     NELNIEYIILTHGHGDHIGYVKDIKEKTGAKVVAHVDEKELLNDKNKNLSYTMRCGAQ
                     EFDADIYVNDKDKLELGELKMTFLHTPGHTQGCMCIRVEDEMFTGDTLFAGSIGRTDL
                     YSGDFNQMEKSLKKLCKYEDAVRVYPGHGPTSTLGVEKKTNPYM"
     gene            complement(28879..29328)
                     /locus_tag="PC471_000026"
     CDS             complement(28879..29328)
                     /locus_tag="PC471_000026"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HDF2787178.1"
                     /translation="MRAVVQRVSSSKVTVDENTIGQINKGLLVLLGVTHDDKSSDVDY
                     MIDKILNLRIFEDENDKMNLSLMDIGGELLVVSQFTLYGDCRKGRRPGFSNAARPELA
                     NNLYEEFVKKAKDKGVTVGTGQFAAHMMVELTNDGPVTILLDSSKSF"
     gene            complement(29353..31560)
                     /locus_tag="PC471_000027"
     CDS             complement(29353..31560)
                     /locus_tag="PC471_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003229747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HDF2787179.1"
                     /translation="MHDKELQEIIEKIKVYAPNANFEMVEKAYNLAKKAHEGQYRKSG
                     EPYFIHPLAVANILIEMELDIETITAGLLHDVVEDTEYTYEDIKKEFGKEVADLVDGV
                     TKLGQIKYRSKEETQSENLRKMFLAMAKDIRVILIKLADRLHNMRTLKYMPPEKAKYK
                     AKETLEIYGGIAHRLGISKIKWELEDRALRFMDPEGYYDLVSRVSMKRSQREDYIQGI
                     VELLKDKFKDLSIPCDVYGRPKHFYSIYRKMQKKHKTFEEIFDLTAVRILVDNVKDCY
                     AVLGIVHTLWRPIPGRFKDYIAMPKPNMYQSLHTTVVGPDGEPLEIQIRTHEMHNIAE
                     NGIAAHWKYKEGISSKESKVEEKLQWLRQMMEWEKDLKDPQEFMDALKEDVFNSQVYV
                     FTPKGDVIELPAESTPIDFAYRVHTNVGNKCVGAKIDGRIVPIDYKLQNGNIVEVLTS
                     SNSNGPSRDWLNIVKTPNAKSRIRQFFKKERREENVDRGNTILEREFKKHDIPQKDPL
                     VEKYMALVAKKFNQPSVEDLIATVGYGGIMSSQVVSKVKDLYLKEVKKAKKEQQIQEE
                     DLNKHNISETEYSKKRKKNASQGIIVKGLDNILVRFAKCCNPLPGDEIVGYITKGRGV
                     AIHRADCPNANNNGDFFQNRLVEVSWSSAGKGKFEAEIQIKAIDRRGIVNEITHIVAM
                     DKLSLNGINARKGKDSIVSINLLVEVNDTEELKILMKKLKAIPGVESIYRMTN"
     gene            complement(31634..32146)
                     /locus_tag="PC471_000028"
     CDS             complement(31634..32146)
                     /locus_tag="PC471_000028"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HDF2787180.1"
                     /translation="MDLKNFIRNIDDFPKPGIDFKDVTTLFKDGDAFKYAVDSIVEEL
                     KDKDVDLVIGPEARGFLMGTPVAYALGVGFVPIRKPGKLPGEVESYEYGLEYGTDTLE
                     IHKDAIKKGQKVAIVDDLLATGGTMEAAAKLVEKLGGEVVSMQFLIELKFLNGREKLS
                     NYDVNSLIKY"
     gene            complement(32159..34609)
                     /gene="recJ"
                     /locus_tag="PC471_000029"
     CDS             complement(32159..34609)
                     /gene="recJ"
                     /locus_tag="PC471_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HDF2787181.1"
                     /translation="MKKWILKYKGKIEESEFSKKLNISPEICQILKNRGICTEKDSEI
                     FMNPSLDYLRDPFLMKDMQKAVDRIKLAVEKNEKIWIYGDYDVDGVSSTSILCLYFKS
                     IGYNVDYYIPNRLEEGYGINEDAIKLISSRGCDLIISVDCGITSVSEVNTANDLGIDV
                     IITDHHECQSEIPSAFAVINPKQEDCNYPFDSLCGCGVAFKMIQALTPKEEFKTSMYN
                     YLEIVTLATICDIVPLIDENRIIVKNGLKSMKEGKNIGLRELIKVCGVESDKIGSSHI
                     GFAIGPRINASGRLGYSYLGVELFTTQSQEEAVEIASILEEKNNERQMIEAKMYHEAE
                     EMLKSNSRYNDDKVLVLAKEGWQHGIIGIVASKLTEKYYKPTILLGIENGEATGSARS
                     IKGFNIFEALIKCKDLMTKFGGHEQAAGLSLDSDNVEILANEINKFADYNLTEDDMIE
                     NVNVEFELQENVINLNLVEELHKLEPFGLNNPNPRFIVRNYILKDLKVIGKNQQHLKL
                     SIEKEKSYECIGFNMSHLKSMYKVGDKVDVLFQLDENNYMGNRKVQFLLKDIRLARPK
                     SASNDKLSLKLMSKIIPKDTQSLYNISVSDFELFDGNTDINIFDYFEKDTLIISNSIN
                     GFYRAMSDISLIDLDFNINYNIIEDVSKNTDKLELIFSPNIDKIDLKRYNNIILYDYL
                     YNKGEYSYIYENKREESEIIKYYNKTDLLYLKNVVSNIVPSRDEFITIYKQALIKKEI
                     DLDMVNIRETFNVIPLKFFTILNVFRELNLLDFNLNYEKNSVLIRILPKPQKKLDLNE
                     SLILNNLKNLEKQYNSSY"
     gene            complement(34622..36286)
                     /locus_tag="PC471_000030"
     CDS             complement(34622..36286)
                     /locus_tag="PC471_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HDF2787182.1"
                     /translation="MRISYRNLKRYREIGYVLIKYGFSFIVERLNIEGIAYKIPLFDP
                     PEEIKNMTTGERMKRVLEELGPTYIKIGQILSTRKDLLDQDIIDEISKLRDDVEKFDS
                     NIAIDIFKEEVGLSIEEIFLEFKEEAIAAASIGQVYEGVLKTGEEVIVKIQRPNIEKI
                     IKSDLEILRTIANTLKDLKKDFNLDLVQMIEEFQTQLMRELDYTFEAINATKFSRIFK
                     NSDEVYIPKVYSEYNTKKILVMEKVNGTKLSDVEKIRRLGYNTKTIVEIGVRSFFTQV
                     LSHGFFHADPHPGNIFVVAKNKIAYIDFGMIGIIDNKTLNQLNEIALAGVEKNVDKII
                     YLLIEMDALNGEADIKGLRQDLLYLIHYYYDISIEKINVTDILNELFRFFRQYKIVMP
                     AQFVTLAKTVITLEGTSRTLNTDFSFGSMGKEFMKHHYKSKFNPKNVVLSSRQNVEEI
                     LLDIKTIPKQLKAILKNIERNNIKMQIEDVKMTRLENCIIELTSQISLSLVLASIIVG
                     SSLIIASPNIENNIWIKFTAIAGFFISFIIGLCLVIRSIRSKYKKD"
     gene            complement(36584..37081)
                     /locus_tag="PC471_000031"
     CDS             complement(36584..37081)
                     /locus_tag="PC471_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HDF2787183.1"
                     /translation="MIRDYLEDKPLIDESVFVAKSADVIGNVKIGKDSSIWYNAVVRG
                     DEGPITIGENTNIQDCSIVHGDTETIIGNNVTVGHRSIVHGCKISDNVLIGMGSIILD
                     NAEIGEYTLIGAGTLITSNKKFPPGVLIMGSPGKVVRELTEEDKKYIDESYEWYLEAA
                     QNQKY"
     gene            complement(37110..38489)
                     /gene="scfB"
                     /locus_tag="PC471_000032"
     CDS             complement(37110..38489)
                     /gene="scfB"
                     /locus_tag="PC471_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioether cross-link-forming SCIFF peptide
                     maturase"
                     /protein_id="HDF2787184.1"
                     /translation="MSMIHKFSMNGYNIVLDVNGGAVHVLDDVAYDLLDFYKEKSKEE
                     ILEILKSKYQEEKINEAYEEILNLEKEGLLYTEDTYQYHPSFVHREPVVKALCLNVAH
                     DCNLKCKYCFAAQGDFGGEKELMSFEVGKAAIDYLIANSGSRKNLEIDFFGGEPLMNF
                     EVVKQLVDYGRSVEKDYNKNIRFTITTNGVLLNDEIIDYINENMHNVVLSLDGRKEVN
                     DNMRPTLNDKGSYDITLPRFKKLVEKRAKDKYYYIRGTFTRDNLDFSKDVMHFADLGF
                     KLTSVEPVVGDESNPYALREEDLPKIFEEYEKFAVEYADRQLQGDGFKFFHFMIDLNQ
                     GPCVIKRITGCGAGNEYLSVTPNGDIYPCHQFVGNEEFKMANIFDEEIVLPENLKNMF
                     REAHVYTKEECKQCWNKFYCSGGCHANAINFNNDISKPYELGCEMQRKRTECSIMIQA
                     KLMLEGATN"
     gene            complement(38785..38931)
                     /locus_tag="PC471_000033"
     CDS             complement(38785..38931)
                     /locus_tag="PC471_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic lactone autoinducer peptide"
                     /protein_id="HDF2787185.1"
                     /translation="MKKFIVRFMKFASSLALSTAILSANSTCPWIIHQPKVPKEISNL
                     KKTN"
     gene            complement(38961..39539)
                     /locus_tag="PC471_000034"
     CDS             complement(38961..39539)
                     /locus_tag="PC471_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HDF2787186.1"
                     /translation="MFKRYAEKMTSVLICNNMIDNNESKVYSYGFEILIAFIVNITTM
                     LFIGFLFGKFTYVLFFLMCYCPIRQFSGGYHADNYFRCLLTFIFIILSTILIIENINI
                     DLFKNIIMIIASVSWVGICVLCPIEHRSNPISDREKLVYKKTAIFISTVVLLITISSL
                     SLSIFVDYFTYSAFAMFWIFVMLVLGKLKAKV"
     gene            complement(39907..41337)
                     /locus_tag="PC471_000035"
     CDS             complement(39907..41337)
                     /locus_tag="PC471_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4116 domain-containing protein"
                     /protein_id="HDF2787187.1"
                     /translation="MDIKNKIKSIFLPEKNDYLDDEEFEYDIENNEEDLKEDNKVHLN
                     DLSRVKYDYRELKDIENQTEEMCLLAVKQDGTIIEFVKDKTYKVCMEAVKQTYKSLKY
                     ITNQNEDICIEAVKQNYRALYYINNKTENVLIEAIKNASTYDVMEVFKFVEEQTEDVC
                     LAFIERASKNDVAEILKGIKEQTPAICLEAVKKDGKSLAYVKEQSNSICLEAVKENYS
                     ALSCVKEQTEEICIEAVKQNDFALYYVNEQTEKICMEAVKRSYMALQYVNKQTEEICL
                     EAVRIDGRALQYVKEQTEEICLESVRQNGKVLQYVKKQTENICIEAVRGSFEELEIKE
                     ILSYVKIPTERIFVEAVKQNGKILKYVENQTELICLEAVRENYNALAYVKEQTEKICL
                     EAVNQSYEALKYVKEQTEEVCLKAVKQDYRMLKYVNNQTEKICLEAVKQNYRALEFVD
                     NQTEKVCLEAVKQNRKALQYVKQKQS"
     gene            41681..42346
                     /locus_tag="PC471_000036"
     CDS             41681..42346
                     /locus_tag="PC471_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2787188.1"
                     /translation="MKGNIVQYNFADIEEEVYSLDYAIAWNTDEENVNIIPFTNKFCK
                     ESIESFCLGKINNFVEILNEGFVENHHYVHLDKMISVPKKKVNLVYQQDTHGYLLRDD
                     NDNLIPAKITSEQSKSISSKMELFCAGEEKCLINILLKADPSYILDVDSIKDKNILNL
                     GYESIDRYKEYNFDDDKILIFFINKKRYSVIMKKTNNSDNDLVSRNNAIKELFTNKAG
                     NLN"
     gene            complement(43138..44850)
                     /gene="ptsP"
                     /locus_tag="PC471_000037"
     CDS             complement(43138..44850)
                     /gene="ptsP"
                     /locus_tag="PC471_000037"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HDF2787189.1"
                     /translation="MAYKGIGASPGVALGKALVVEHSELVIEKKSIDNVEAEIAKLED
                     AVAVSKEELVKVKEKASEELGAEEAEIFEAHLLVLEDPELIGSAIDKIKTESVNAEYA
                     LNEIKEMFVSMFESMDNEYMKERAADIKDVTNRILRHILGIKVVDLSALSEEVVLIAH
                     DLTPSDTATMNKKMVLGFLTDIGGRTSHTAIMSRTLEIAAIVGLNDITSKVKDGDFVV
                     FNGDTGEVIVNPDEETINKYTELKAKYEDERKALQLLKGKPSVTLDGKHVELAGNIGT
                     PNDIEGLIKNDAEGVGLYRTEFLYMDRDSFPTEEIQYEAYKAVLEGMDGKPIVIRTLD
                     IGGDKELSYLSMEPEMNPFLGYRAIRLCLDRKDIFKTQLRALYRASVHGRLRIMFPMI
                     SSLEELLQAKEVVKEVLAELDSEGVAYAKDVEIGMMIEVPSAAVISDVLAKHVDFFSI
                     GTNDLIQYTCAVDRMNQKISYLYNQFNPAVLRLIKTVIDNAHKEGKWAGMCGESAGDQ
                     KMIPILLGMGLDEFSMSPISILPARKLITSVKESDMKKLADDVLNMGTAEEIKSYIEK
                     TFNI"
     gene            complement(45052..45510)
                     /locus_tag="PC471_000038"
     CDS             complement(45052..45510)
                     /locus_tag="PC471_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2787190.1"
                     /translation="MKKTIALLLTLCAVMIPFTAFADGNDYEGTGNSQTVELIGQPNS
                     DSPSTKSVVVPGGTANLTAMSGGQLSWRVTSTLGNILGFEGNIDVYKAGKIVSSQYVG
                     FRLAGTTKTGVVTFKGLKKGSYIAKFKGKGVCTGGIILFSSPAEASFTIR"
     gene            complement(45545..45847)
                     /locus_tag="PC471_000039"
     CDS             complement(45545..45847)
                     /locus_tag="PC471_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2787191.1"
                     /translation="MEVNLHTGKDEFFKSHEVAKNYIGGLYKLIDVYKFSMESVSVLT
                     RIDLDKLNNFYAGEASLNYDELCLIESVIITPLVGAFETAEHNYNMIIKARKERKL"
     gene            complement(46235..46387)
                     /locus_tag="PC471_000040"
     CDS             complement(46235..46387)
                     /locus_tag="PC471_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2787192.1"
                     /translation="MDNFLFNVLASLTASVVVYLISELFKKAKSHSRAKSDLQVEFKF
                     IFKFKK"
     gene            complement(46507..47241)
                     /locus_tag="PC471_000041"
     CDS             complement(46507..47241)
                     /locus_tag="PC471_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HDF2787193.1"
                     /translation="MYYVYKYINLDTEECLYVGKTENICDRHASHLSNKKENWCNKNL
                     RFEYIELDNKYTMDFYEIYLINKLEPKFNITGKGEMDIAKTSFSYNGQWVIYSERDLM
                     SSLASKRCGINYEVNAKMLGIMRKLKKISSNEEIFIGNENIRIKYYFETVLEGINLEP
                     CILSVAYKTLKESTVGTVISVKREYLSENVCLIIDSIFLNEIMKDPLMSKSDIKDLNS
                     QVNDILKIIGVDCEWNKLSDYCSANS"
     gene            complement(47261..48448)
                     /locus_tag="PC471_000042"
     CDS             complement(47261..48448)
                     /locus_tag="PC471_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HDF2787194.1"
                     /translation="MEKKEILMQPNNLIKSKYDFTNVENKLFYKILFNAQKQQNSSYV
                     TTISKEELKVFMKNNNDYEHKNIKEILNMFQQSVLEFDYIEETTGKLKTFGSGLINTY
                     ELDHTDQIYTIMMHEVLYNHITDFVKMQKKKNGYTAINLSVLFNFRGAYTQRLYTLFR
                     LWSRENKEVEIKYKLDELRFYLKLKDNVYPEYKYFKQNVLKRAMNEINKKGNMVVSIK
                     EEKRKNRKIDEIIFSVIDYEPRKYFDKDILVEDYIPKKKQDNEVSFSFYIPNENIFTT
                     GTIKLFKEDFKEFDFKKDVYLNAFNKSIAIAFERDNTDIINTKNYDFFKSTLSNKISE
                     VIIHQKDDLKFKEELDKYWDDEDVKKEEREVDYSKDPVRLETVRRNLFDQGLVNEEGY
                     YYK"
     gene            48868..49557
                     /locus_tag="PC471_000043"
     CDS             48868..49557
                     /locus_tag="PC471_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HDF2787195.1"
                     /translation="MLENRKQIQKEIYLPIATYCLKDKQYYIKEYGALLIKSAENSDI
                     FNTHKNRILYKNKLDIQEIANELNVNKATIQRNIKKLEKLDFKILKIENTLNGIVYCL
                     PSENNIDLNKFALINYEMLKKMVNKFKSNTIKVYFLLKTITTETNFKPATNSFIAENI
                     GLSSKSKNNLDIITSSVKTLEENKYIETKKVNVYEYDKEKLREVPKIRKVYRICNFDE
                     WKKEIDKNKVY"
     gene            complement(49706..50089)
                     /locus_tag="PC471_000044"
     CDS             complement(49706..50089)
                     /locus_tag="PC471_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HDF2787196.1"
                     /translation="MKIKKIPESELLVMMFIWDSEDKEVASTEILKTLGEKYEWKKST
                     MLTFLRRLVGRGFLEVVKKDRFTYYKALIEKEEYLKVETKSFFSFFHKNSFESFISAL
                     HDDEEVSENKLKSLEKFIEDWEEDK"
     gene            complement(50803..51189)
                     /locus_tag="PC471_000045"
     CDS             complement(50803..51189)
                     /locus_tag="PC471_000045"
                     /inference="COORDINATES: protein motif:HMM:NF024252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2787197.1"
                     /translation="MIDEMKPENKRIRELRLNLNLSQEEFANKLNLKRNTISLIENGK
                     RNASERTLNDICKTWSISEEWLKHGVGEMFFIKELDEQFAHLVGELSKMENEVEREII
                     TKMFKLNKKYLTVFDKMIDSLLEIEE"
     gene            51337..51480
                     /locus_tag="PC471_000046"
     CDS             51337..51480
                     /locus_tag="PC471_000046"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2787198.1"
                     /translation="MTDINKIREEIYRKLELLGPAKLKLALTKVDILKEIQDIEDENN
                     IQN"
     gene            51458..51709
                     /locus_tag="PC471_000047"
     CDS             51458..51709
                     /locus_tag="PC471_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009900161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2787199.1"
                     /translation="MKIIYKIKGVKRLKISDFNKNQIGSAVQLQDIRFDTKADCIITK
                     VEKNEILVMYYEKETEEIAYKTLTKEDLILDDYKLKLLS"
     gene            51737..52468
                     /locus_tag="PC471_000048"
     CDS             51737..52468
                     /locus_tag="PC471_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009899661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF2787200.1"
                     /translation="MDLLKDARKKEWFWLENDLIDREDLSIYEKMIYIVLARYSNEES
                     SCFPSYKTISEKAGCSIRQVSNILNELENKELIIRENRNHEGKKEKNSNLYFLISSKA
                     KVQNEVPNVRHEVPNPMAPGALHVRHEVPSKKTYNKKTYIKSNTTTQKESKTDYLDLS
                     FLDLDIEKVKLTKDEYDKLISKFGKKYIHDKIVSLENYIINGKGSRYKSHYRALLTWG
                     NGDASKGIVPEVTKTKNPLSGFKEL"
     gene            52496..52681
                     /locus_tag="PC471_000049"
     CDS             52496..52681
                     /locus_tag="PC471_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2787201.1"
                     /translation="MNEKEETVYLNSSNVKLFLGVRDIKEFEELIENVNERICQLQES
                     INKLAQFNIEFDIKSDI"
     gene            52707..52925
                     /locus_tag="PC471_000050"
     CDS             52707..52925
                     /locus_tag="PC471_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009899664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2787202.1"
                     /translation="MKDEKLELTEKDLHCIARHLQNEVMEMVFRGNIEAPTSCEVCDY
                     LQECKDYFTHIDTFIKLSEMTGVDIFTK"
     gene            complement(52944..53156)
                     /locus_tag="PC471_000051"
     CDS             complement(52944..53156)
                     /locus_tag="PC471_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2787203.1"
                     /translation="MNMCKIKTLREKCSLIDDKVEFSGEYKTFPGFTGSKLLECECNK
                     NYICKIQIMTANSNCPLFKKARQLQY"
     gene            53319..53579
                     /locus_tag="PC471_000052"
     CDS             53319..53579
                     /locus_tag="PC471_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2787204.1"
                     /translation="MKEEKLELTEKELHCIARHLQNEVMEMVFRGNIEAPTSCEVCDY
                     LQECEGDFTCMYHSFTKLSKITGVKVTAFKYCFPDENPKATI"
     gene            complement(53572..53760)
                     /locus_tag="PC471_000053"
     CDS             complement(53572..53760)
                     /locus_tag="PC471_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2787205.1"
                     /translation="MSQYRVISKECPVINETISLTCEYSAPPGTFGSKLMSTRCDGSH
                     ICDSSSNEECPILKKLLR"
     gene            53898..54215
                     /locus_tag="PC471_000054"
     CDS             53898..54215
                     /locus_tag="PC471_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2787206.1"
                     /translation="MERKKHEFTDQDIQRLIEGLRTDPKIREMIFKSKGCETRTSTRS
                     KCEQFVEEKLAYRSYGFETPKYYAKLIADIIDILVYKNVPRDRICGVLYDVERVIPLV
                     VTF"
     gene            54237..55568
                     /locus_tag="PC471_000055"
     CDS             54237..55568
                     /locus_tag="PC471_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009899669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDF2787207.1"
                     /translation="MNYSLEAEQNILGSFLINDSIGYKIRELKENDFYFESHKIILSC
                     MKKIIDSNKPLDLLLLKNELEKIDRLVDIGGVSYITSLTTIVITTSNIDHYIKIIKEK
                     LLKRQISELANELINNSNSDTSIDELIIDINDLKALVTTSSSVNDNYIDASKIKREKG
                     VHKSIDTGFNKLNNMLDGFRYGTLTILTGKPASGKSTIVNQFIAQAITNGEKAFLYSG
                     ELPSFMSMDWFRKTVANDYHIKEYKSVYGGTYTDIPDYAVELISDWIEDKFFLYDEDA
                     ISDEVNLLNTIEHLYLKKGVRFFVLDNLMTIKTGNKADKYERQEQIVSNLKNLAKKYN
                     LVIVLVAHPRKNMGDMKPTMYDVSGASEIVNYADYILSTYRVVDEEEETDDTYLLILK
                     NRITGKQNISFKMNFSERRKRLYTTSEELNRDYKYDVNKQYVQVEIPEDVF"
     gene            55943..56329
                     /locus_tag="PC471_000056"
     CDS             55943..56329
                     /locus_tag="PC471_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2787208.1"
                     /translation="MIALEKFAGGVLKEKFNTELQKVLDNIADPNTDFKKTRKISLEI
                     VFKANEDRDLAEVDIKSKATIVEAKATTTKVLIGKDLETGRVEASEFKNQVAGQLVMD
                     IPDSEETNETEENSAGVIDFRNASSK"
     gene            56382..57086
                     /locus_tag="PC471_000057"
     CDS             56382..57086
                     /locus_tag="PC471_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2787209.1"
                     /translation="MIRNAMEYLVKLGKRETVCVDGFKYTTDALEIIKEPKANELEIT
                     TLSGLVDYIKSGIDHKADELLLIQVLTPRKVLLKSPLRKNRDRETYIECVALLPEKRF
                     DRPLDLDSFNIMLQSAFIKNNDRDTLLKIAGNIQESTVKNVGDDGVSQSVTIKTGVAS
                     VSEAIVPNRVKLIPYRTFQEIEQPESEFIFRMSSGPAAGLYEADGGAWKNQAMLNIKA
                     YLQEELKDFKNVNIIA"
     gene            57143..57325
                     /locus_tag="PC471_000058"
     CDS             57143..57325
                     /locus_tag="PC471_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2787210.1"
                     /translation="MKMISHMNILERAEFTDKIANAIMSANKEIERGCAFNEAVEIVK
                     QMEYRENKYNEGDIAI"
     gene            57322..57699
                     /gene="ssb"
                     /locus_tag="PC471_000059"
     CDS             57322..57699
                     /gene="ssb"
                     /locus_tag="PC471_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HDF2787211.1"
                     /translation="MNNVVLVGRLARDPELRYIPEYGTPVATFALAVDRGYTKKDGTR
                     EVDFIPIEVMGGSAEFCANYLAKGRMVSIQGQIRIEKYEKDGEKKTFTKVRTKIVNAL
                     DRKPKDEEKEISFQVLDDEDIPF"
     gene            57680..58264
                     /locus_tag="PC471_000060"
     CDS             57680..58264
                     /locus_tag="PC471_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014466311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HDF2787212.1"
                     /translation="MKIFHFKLDGDYVRSLEERNKLVEENINFVWFIIRRYFKVFISK
                     YPYLQDDTFSEGCIGLCKAADYFDSKKGIEFTTFAAVCIKGQIFKFISRYVKKHYRAD
                     IDSFEKCIYKNTHGEEIRIEDRLAGNENVDIENVHVIRSCIKRSKIEDIQKITYLREK
                     GYTQKEIGKILRTNQMTICRKLGKLKSEINILGK"
     gene            58294..58452
                     /locus_tag="PC471_000061"
     CDS             58294..58452
                     /locus_tag="PC471_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2787213.1"
                     /translation="MKTKIIGGRENQCPLCNGSVFKIETSIGIVWQCKDCGCMYQDMN
                     CKENKRRH"
     gene            58455..58781
                     /locus_tag="PC471_000062"
     CDS             58455..58781
                     /locus_tag="PC471_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2787214.1"
                     /translation="MACVIKCDFCGETIAEILNIKVFRIDDEGNIMSIGKDVCDKCYD
                     RLFRSNLNKKMTNYEKIKNMSKIEMAEFLANGGSGCTSCAYDFQECLGGCLEGRKKWL
                     ESEAMK"
     gene            58781..59317
                     /locus_tag="PC471_000063"
     CDS             58781..59317
                     /locus_tag="PC471_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HDF2787215.1"
                     /translation="MKKTLIIVGVVISILIMFACLFLVTKNKAIDLEEQVQSATASVE
                     VQEKRRIDLIKNIVDCVKDYAKYENRTLKEVTDMRVSANDESIKEAKLNIQAVAEKYP
                     DLKANENYKQLMSELSITENMIAQHRENYNMQVRNYNKYIRKFPANLILNSMGYEKIE
                     SKYTEYKAKEDAPQNLFD"
     gene            59329..60135
                     /locus_tag="PC471_000064"
     CDS             59329..60135
                     /locus_tag="PC471_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2787216.1"
                     /translation="MKFKTFEITKREFLVSIIIACMMIVLGFFIDDKVQSKFLEDNER
                     YYKATKINNDKDLFDYAMETSAGDSLISGRFKSVDSVSIEELKGDYWFIERTEEKYTM
                     HTRVVTTKDSKGHSHKYTETYWTWDYHDSIEKKCNKFNFMGKEFDAKVITNLSKERLA
                     LKKDIFKNNNLKIKGNYAYINNKRRYYYEVIPKEFNGTMYANLKNNTIANPADKNIKV
                     FKGESIQNVLENNNFNTGFFKVVFWIIWILITTIIIYVFYYLKNDWLEDK"
     gene            60160..60420
                     /locus_tag="PC471_000065"
     CDS             60160..60420
                     /locus_tag="PC471_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2787217.1"
                     /translation="MKCKVYHAIKNIKDANELIDRFETENIFFELNDIDNENVLNGEM
                     NLTDIEDIASLQFILDGDLFIFSSCDEMIFMNKYTQKELGLQ"
     gene            60444..60929
                     /locus_tag="PC471_000066"
     CDS             60444..60929
                     /locus_tag="PC471_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2787218.1"
                     /translation="MDLKLISSIPNWKVEDQLAKIDEEVTEFKNAIDTGITKDIIAEG
                     LDVCQTILTMFQVLEIENYITEGVETHNKKLNGRGWDLKEIGDDILNLIKNSMINELI
                     NMNKKLKCLDEKEFNAKIQFLDVDKLWNLCMPLVNLSTIKGVNESIDEMKMGEFLKNI
                     N"
     gene            60949..61206
                     /locus_tag="PC471_000067"
     CDS             60949..61206
                     /locus_tag="PC471_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2787219.1"
                     /translation="MLIEVTENNRELKVGDIVEINGGNKLYMILSLKETIGGYVIINM
                     QNGYGSFGAYKSLSQLELDLKVRGYKLYSSDDYKLQLVPRE"
     gene            61315..61758
                     /locus_tag="PC471_000068"
     CDS             61315..61758
                     /locus_tag="PC471_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase subunit sigma"
                     /protein_id="HDF2787220.1"
                     /translation="MQSSKKDNLFSSAEGKLYDYKKIKAEIEKIDIDMQMIKNDYRGC
                     SGVEIKEKTGKTYDIKSVIEIETEEKEKKIALKEKEKRHKELIIEKIDNAMKILSEEE
                     KKIVQYKYFSSSRTSWEYVGRMIGFSASKCKQMRFDIIDKIKGLL"
     gene            61901..62446
                     /locus_tag="PC471_000069"
     CDS             61901..62446
                     /locus_tag="PC471_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2787221.1"
                     /translation="MAWNLRSKSFLLSREVLKIKRDITEEISSAAYVPDNLKYYDNVM
                     KETPGMYGRECSLDFIKKKQEKLLKLKKKVKKNYDSNINKIDSYLKILEKSIIDESDH
                     VELVVFKLYLQLENVVKVTETVNDLGFRIKTNTYARERKYSTNDITSIITDPFTDVVE
                     DLKILVQERQRKNYYGNKECF"
     gene            62450..63094
                     /locus_tag="PC471_000070"
     CDS             62450..63094
                     /locus_tag="PC471_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDF2787222.1"
                     /translation="MEAHSGQKVNPIKDIEDVFRLLNFLEEWNERNYLLALFGMCTGL
                     RVGDILALKVADVTDLKLDKKGNKIRVSKDWIRVIEEKRDYNREVFLSDVIKSAIENY
                     TQDKPGEEFLFKSGKRKKFNRPIQTRQAGRIIKGAAKKVGIKENVATHSLRKTFARHI
                     YDEEENKTYALELIRKILGHKTIEMTRRYIGIDKDEEVKAITKFTNKLKKRKRN"
     gene            63309..63767
                     /locus_tag="PC471_000071"
     CDS             63309..63767
                     /locus_tag="PC471_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF2787223.1"
                     /translation="MDVNEVEIIDSSYEKGKELTEDQCNMVTLLITGATVTETARIIG
                     VNRKTIYNWMNKEHVRKEMDRRKQELTNQGNLMILKDLDSYINNIKELASDKSDKRVM
                     LAANQYLINRIYGTPTSTVIQAEDNSVGMGMDATEIEAAIAKIRIRTSKK"
     gene            64322..66061
                     /locus_tag="PC471_000072"
     CDS             64322..66061
                     /locus_tag="PC471_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2787224.1"
                     /translation="MDNLERNIQLIFSYLAKTFIRNGASIKDAEKEANEIIKANSNNL
                     WGVNGLAYQLGKINLEFFCMYFMQDTYLPKEDNAAAPIAKVHHELWEDIQESIIGDGS
                     QQLGRIYPRGTGKSAFGDLATTVWSHCYKHKTYTLICSDIGSTAEKFVKDIKNALLEN
                     EYIKKAFGVLLNDNDRKYICNSTQLELTNKTFIEAISSSSPMRGRKYNNNRPDLIILD
                     DYQSEENVRTEDAREKKFKRFSDDVKYAAQKPVIRNGKTIKRGTTFIALGTLQHKECF
                     YSRLKNLPTWKFKCEKGVLVDNVDELFNSGLWLKFKEILFDFKNTNHLEDAKEFYWEN
                     EKEMKFPILWPSFWDCLDMALSYYENPTSFKQEVQNDVNSIGEKWFKTVRTETREEIE
                     SHTFKKTMLLCDPASAGGSKHDYSAFLVGSESENGLLYGRLAELAKINARTDFDKYID
                     HMIYLLKVYSDITHIYIEKNTFNGADANQLEFKIKNDNVLSYRDIEIINEQQKKNKDD
                     KISTLIPVLSKGQMIFAEEDKEFIQQILDFTGQKYSLHDDAPDISSEFINRINNIKVS
                     ETVNIFDRKLLGF"
     gene            66062..66265
                     /locus_tag="PC471_000073"
     CDS             66062..66265
                     /locus_tag="PC471_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2787225.1"
                     /translation="MGGNLLANRSKFEEVQKTLTKKGKKFNVGIGKDEKGYFAYTHRA
                     RSKSYISKQDIPIKVLKFIESTG"
     gene            66278..67555
                     /locus_tag="PC471_000074"
     CDS             66278..67555
                     /locus_tag="PC471_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009899679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HDF2787226.1"
                     /translation="MELDLDLIKDIYDDWNSNKSEYDTMYKYYKGETDAISNYKMVTK
                     RSNNKINTNFLKKFINEEVAYSLANKITYTSRSGDEKIINDLEYYTCHWSKKHDSDLL
                     RYALLFGLCYELYYVKDDEIQVKIIKPTDGCHYENENGEIVCFIREFTKGFEDDTYVD
                     VYDKEYIYHFDSNFKEVESPTVNNIFDGNVPISICKRSEELGKNTIFNDIKGLQDAYE
                     TNLSDISNEISDFRNAYLTFSGCNIKEDDLPRMKELGILQVNGDGKIEWLIKDMNDTF
                     VQNTLSSIKENMYEITSHINHNEKMQSNTSSLAVIARLISTEWICSQNNDSIADTLFN
                     RYKLLCIWLNKKYGFDYDYKDIKAKFTPKIPRDDLVVANILSQLGDKLSTETGLSQLS
                     FIDNPHAEIEKAKKEQEIVSEGEILLDESKDYN"
     gene            67533..68294
                     /locus_tag="PC471_000075"
     CDS             67533..68294
                     /locus_tag="PC471_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HDF2787227.1"
                     /translation="MNQKIITKLTEDIYKSAENRTKPFYKYQRENRDKLLLEIAKILL
                     THDIINEYLNINNKDKKNLRSKLNKIINEYSNSYDEEVKDIETILSDSSKNKYSNLII
                     LLGVILKIKDVSNINNKVINKIVNNKVDGKHWSDRLWKNKKGIEESLKKEIKSFLNGK
                     TSVNKIEKAIRDKYSTGAGQTSRLIENEVARCQSEVNEYFFKVQGIIKVMYCADLDSR
                     TCSDCSMHNGVIFYVNEDRPSLPRHCHCRCQYIPV"
     gene            68353..68679
                     /locus_tag="PC471_000076"
     CDS             68353..68679
                     /locus_tag="PC471_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2787228.1"
                     /translation="MKVEILGTEYLVIEKLESEDVLLKERAGYCDHSVKEIVIEKINS
                     EEGSLKDLSVYKNEVARHEIIHAFLSESGLKSCSSWATNEEMIDFFAIQFPKIVKVMD
                     KIECLN"
     gene            68705..69307
                     /locus_tag="PC471_000077"
     CDS             68705..69307
                     /locus_tag="PC471_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4355 domain-containing protein"
                     /protein_id="HDF2787229.1"
                     /translation="MLKKEVLELLKEIEDDAEIDSLLQSTDLFKSASDKKLTIEEFRE
                     LINNDANFKAIIDNEKNKYHSEALENFKKKDMQTLISAEVLKRTGANETEEQKAIREL
                     RESLDKLKKEKQYAEKVSKYKDILVEKKIPTNLIEYLLSDDDDKTNANIEIFENSMKQ
                     YVQSKVDERIKDGSYVPPGKDSAGSLSEIRKQIKQGLNSL"
     gene            69336..70259
                     /locus_tag="PC471_000078"
     CDS             69336..70259
                     /locus_tag="PC471_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2787230.1"
                     /translation="MANVLQYVTLFQQELDMQVTVGSTTGWMEKNAGQVIYNGGREVK
                     VPKVTMDGLGDYDRSKGYTQGAVTLEYETMKMEMDRGRTFMLDSMDVNESNFVINASS
                     VMGLFQKQHVIPEIDAYRYSKIFSETAKVDERVKYSYEATVTTILSELKNQLASIQDL
                     SGDIPLIISMSSIIASILEQSSQLDDIEFGDGDIKRIVKSIDNVPIIKVPSKRMKTEY
                     VFRDGKTSGQTEGGFTEGAKALDINWLITPASAPIAVSKTDKPRIFTPDQNQTADAYQ
                     IDYRKYHDLWILDEYLKLCRASVRQAKAAQE"
     gene            70273..70545
                     /locus_tag="PC471_000079"
     CDS             70273..70545
                     /locus_tag="PC471_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2787231.1"
                     /translation="MFALIKNNIIKVVEDENSRDKLISKGFKIYSEEVTKENIEKKEE
                     KVKSTSNDLFNLSKPELMEIAKGKNIKGYSTKTKEELIQLLDEYNA"
     gene            70529..70909
                     /locus_tag="PC471_000080"
     CDS             70529..70909
                     /locus_tag="PC471_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail connector protein"
                     /protein_id="HDF2787232.1"
                     /translation="MSTMLDNVKLVLGIENDRYDKLIILYINKISEMVLEYCSRQELS
                     TALEGFVEEKVISILSSRFEELKKNSGLDNLEKSLKAITRGDTKIEYNALTLQTAENS
                     ISSSSFLTDTDKKFLSPFCIVSMY"
     gene            70921..71268
                     /locus_tag="PC471_000081"
     CDS             70921..71268
                     /locus_tag="PC471_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2787233.1"
                     /translation="MTEADILEMTYLDKMTIIRKLKGWNEETCSNDFSNVVIAENIPC
                     AISRKEEAIVNGDIGSIVVNRKLFCRPEVDIKIGDTINTTYNHGEEAVFKVSKANIYP
                     SHMEIPITELERI"
     gene            71268..71624
                     /locus_tag="PC471_000082"
     CDS             71268..71624
                     /locus_tag="PC471_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HDF2787234.1"
                     /translation="MGIDNLGVFEALALDLGLNASNFNRKIKKFTKETGQELAENIRD
                     ETPVKTEKLKKGWEEKQPEYDTYIVKNEVDYATLVNNGHSTKNGGIVPGSFMVEKGIE
                     RTKENMTSAKANLFDW"
     gene            71633..72082
                     /locus_tag="PC471_000083"
     CDS             71633..72082
                     /locus_tag="PC471_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009899640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2787235.1"
                     /translation="MLTNRDIIIAVNKRIENEFRYIDIFINEDNIQGFEEACFFVQLL
                     PVSNNIANKVLDTKKIFIDIEYYQKARKNKLNLYDIQSRLEKIFNRNIRVKDRSLIIE
                     NISPSIAKDPVGYKLTFLMTVSYFEEVYFENETFEIMESVDLEIGSD"
     gene            72083..73138
                     /locus_tag="PC471_000084"
     CDS             72083..73138
                     /locus_tag="PC471_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HDF2787236.1"
                     /translation="MGLPEINISFIQAGKTFVKRSGGIVALILKDTKNIGLVEIKEIE
                     DIPENYSAANLDYIKMAMKGNVYPPNKVLVYTLDTDESIDDALDFLETCEFNYLCMPD
                     ETEQDLPKIKTWIKKMREDNKIKVKAITASNSADYEGIINFTATDIEVEGKKYTSNEF
                     LPRIAGFIAGTPSTQSVTYAEIPEVTNIPKLTRAEANTRINNGELILIKESGAIVIAR
                     GVTSFTTITDSKGDIFKKIKLVDTLDQIHNDIKKIIVKNYIGKTQNTYDNKCLLIVAI
                     QLYLQELEKDGLIDEGSTVEINLDAQRAWLKKQNIDISNMEEQQIKEYNTDTLVFLKA
                     RIKLIDAMEDVYLDISM"
     gene            73154..73594
                     /locus_tag="PC471_000085"
     CDS             73154..73594
                     /locus_tag="PC471_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HDF2787237.1"
                     /translation="MKAKIDAKRIISGTFGECWLDNKQLTSTKGMEAKVDIDKEEIKL
                     CGQIMTTYKMIGATGKGSITLYKISTLFGEYVEKMLNEGKEFKVTIISKLQDPDSYGS
                     ERIALYGCMFDDLTLADWEAAKVGEIEVPFTFEGFEYLDKIKEK"
     gene            73609..74025
                     /locus_tag="PC471_000086"
     CDS             73609..74025
                     /locus_tag="PC471_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HDF2787238.1"
                     /translation="MNTVEKLLNMDAGKLKMPSSAFELYCKKLDDTLEIECKAIDPER
                     FDEIRMNSMDVNSGDVENINIYELKVNTILESCSIFSNMELVKKFSAITPKELIKKLL
                     LSGEIDKLYEEVNKVNGIDAEGDKKRQKERAKDIKN"
     gene            74052..74201
                     /locus_tag="PC471_000087"
     CDS             74052..74201
                     /locus_tag="PC471_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2787239.1"
                     /translation="MYLLFKYKGIMPSVSYHILRGEKEIVSVFMDQELDERKEEIKSG
                     VGRLF"
     gene            74205..77594
                     /locus_tag="PC471_000088"
     CDS             74205..77594
                     /locus_tag="PC471_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009899646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HDF2787240.1"
                     /translation="MSSNTKVLQAIIKMRDEASSTLKRVAANTRALHDSSSQASRALN
                     NVQSNLKSVGTSALKAGGLITAGVGGAFAAATKVGTEFESAMSQVKATFGNSLKPGDF
                     EALSKAARDAGKATTKTAADSANALQYMGLAGWDVQKSMHALMPVLNLSEAASMDLGL
                     ASDLVTDSMSSMCLEVDKVSKSGKVLAGAGLSEYLDKVANASTKSNTSIQQLMEAFNV
                     AGGTFSNLKVPLSEATAIMGILANRGVKGTDAGHALNSIMVNLTTGAGQAGKAMKKLG
                     VSAFDNQGKFKGMSNVLKEVANKTKGMTDEQKNYYLSAIGGKTQLKTLQKLLDGVGNE
                     YDSLKKKIDNSKGALKTMSDVMKDNTKGGLATLKSALEETGITIYYQLKPAIASVVDN
                     LTKMTVWFNNLSEPIKRVITNVMLSVGAFGVLLLTIGAVSLIVSKAIGIFGFFVPIIL
                     RAKVAFRAFVIGMKPLSAVFLAVMGPVGWTITAVIALSVAFTIAYAKSEVFRNKVNSL
                     VKSFTEFAKPIVNFCMPALKQLGEKFKELLSAFGPLFASILNLGSVIMKVLGPVLIGV
                     IGFHIAKLVLILSTLATAFKAVVDTITNVLNGLASILNGVFDLIIGIVTGDSKKMMDG
                     VKQIFDGGTKIIKSIWKGFIDFVTAPIQAIADIADSLFKDKAKWLTDKWRDLRTFLEN
                     PIEATAKISTKTNVNIPGSKAADAAGKSTPNTGGLKQTVANLKGSFSDFKTFIKPMTD
                     SISQGVESGKTKFTELQNKILNDVKPALKVLEDSGVNFKDKFKESITSIKDKFNELKN
                     ILAGNIKTNIDNLVKAFEPLKPHLDNLKDCFDKVKKAITDFFTPAKQVGDTVNKVSQP
                     MDSVKKSTDGIKLSFQSLTQALAPVKAWFQGFMPFLSQIGQQLLPSIGIAIAGVVGGI
                     ILAFTWVLNTLTAIITTVSGIINGIMSFISGIINVIMGLITGNADQTMDGLKQIFDSG
                     VGVIKSIWNGLVDLVSGVLEPVIDLADSLFKDKVKGVKEAWNKLKDFFKHPIKGTIEI
                     FKKGPSLQIGKGADAAGKSPRKAFGINRVPRNDYLIRAHEGEKLLTKQEANQYKRNSS
                     NGSLFLDKLADTIIVREEADIDKIVRKINKKISLAKIGGVL"
     gene            77641..78117
                     /locus_tag="PC471_000089"
     CDS             77641..78117
                     /locus_tag="PC471_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HDF2787241.1"
                     /translation="MNKKILVLTISIILSLSLVGCSTNNSKNRDSKSKQNTEEVKNHK
                     IGEVVDLDGAKVSVKNIRKLETNSNDYAVEIEITNTGDNDLQSDYLENYILKSSDNTT
                     GEAINNQELNGELMFDTIRPGDTLKGEIAFELTKDATPKTLEIAYENVGKYTVFDL"
     gene            78175..78810
                     /locus_tag="PC471_000090"
     CDS             78175..78810
                     /locus_tag="PC471_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HDF2787242.1"
                     /translation="MEIWLRQANNTFRFPVIPPSFEINGSATINTSNILSVGDIAVFG
                     GLGLKTIELSSFFPNQEYSFCNYNGFPKPYDCVNLIESWMKEGYILRFIITETNINFE
                     CIIADFNYREQDYSRDIYFTLSLKEYRRIQISKVNINNDEKLSSEKNVPLTKGFDTKQ
                     KTHKVVEGDTLFKIAKKYYSNGDLWEKIYKANEDEIKDPSVIKNGWVLIIP"
     gene            79051..80568
                     /locus_tag="PC471_000091"
     CDS             79051..80568
                     /locus_tag="PC471_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HDF2787243.1"
                     /translation="MIIDRSKDSSNNNIDFTAKDMGFLLLQSEVSYNFKDKLVEDIAK
                     QVFVDNKFPLGNLPKTNVKYTKMFIGVTGYDTIMSVYTEASKTTKKKYMIESNLDKFN
                     VIEKGVVTLNITFEEGFNLINTNFSESIENVKNKVLVVDQYGNKISEKINDSIFKDVG
                     VIMQKVIQQQENSTIDIDGEFKGIEKTCSLKGYGDITCVTGRGVKVKDSYTKLIGLFY
                     IDTDKHTWQNGDYQIELELNFENIMDEKSAGQDEQKEDGSEYTGGTEYPAEFTAYCPR
                     KEEGGNTDCRGKKLDPSKKTCAAPMVGAYEKSYYTKDFLNKHPLMRYGDEIQLVTGVS
                     SRDGTYKVNDNGPAIIIEKDGTYHIDILFGNVEEANNFGRRKGKIIIGGYSGNMTEKA
                     KTVISEARKHLGKPYKWGGNGPSSFDCSGLMVYCFKKVNVNLPRTSSQQSKVGKKVEK
                     NNLQAGDLVFFHNPVSHVGLYVGNGEFLHAPQTGDVVKISKLSNRKDFNTARRVL"
     gene            80582..80914
                     /locus_tag="PC471_000092"
     CDS             80582..80914
                     /locus_tag="PC471_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2577 family protein"
                     /protein_id="HDF2787244.1"
                     /translation="MADPINEFIGIIRQEGKFHNEPSFFIGKIKSKLPDLKIEINNII
                     LEKEDILIDSWMLDRQIELFDTETSQEHKHEIKNPFIDTFESGNTVIMFKIGEKFAVV
                     SKLVSLDE"
     gene            80907..81341
                     /locus_tag="PC471_000093"
     CDS             80907..81341
                     /locus_tag="PC471_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2634 domain-containing protein"
                     /protein_id="HDF2787245.1"
                     /translation="MNNTIFPFMGVPEDYISPDNEELPIFKEFAWDFNKDEKIIENGD
                     FKIVERNEAIKVWIYKTIKTDKYVHLIYDWSYGTDIKNLIGQKYTKGLTESEAKRYIQ
                     EALLTNPYILEVNITNAEFEDDNLSISLNVKTIYGEEEITFV"
     gene            81334..82386
                     /locus_tag="PC471_000094"
     CDS             81334..82386
                     /locus_tag="PC471_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HDF2787246.1"
                     /translation="MYNGQSFSTLNNRTLNNINLPLYKGQGSSLYNIVSPINSELAQL
                     YIELSYIHKRVFIQDNFDDFLDRRVNEFGVYRKLGTEAIGEVTFEGKIGTQIPNGTII
                     SHSDLLFVIIKDITIDENSKLNVSPIQALEIGIKYNLSANTEFKLIEEITGVTKIYNE
                     LDLKGGTEIETDEELKERFYKIQKNQATSGNKAHYQSWALEVEGVYNAKVIPRWDGPG
                     TIKILIYGQNNQSVDNEVLQRCTEHIEEEKPIGPTVTIVTPSTFDINISATLTLENGY
                     DIESIKVVFLDIINSYLIENSREIVYIKVMSLLASIEGIHDIKNLLVNGDVKNIIVDE
                     EKVPAVSSAIFDIEVS"
     gene            82388..82996
                     /locus_tag="PC471_000095"
     CDS             82388..82996
                     /locus_tag="PC471_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2313 domain-containing protein"
                     /protein_id="HDF2787247.1"
                     /translation="MKLIDYLPSFANNEIDIQIQEALENELLTLIDEKDDLLEQFFID
                     TATWGLDDWENLLSIKVNYKLDFDTRRSNIKAKMRGKGTTTIEVIKAISEAYTKTNVD
                     VEVFSNLFSFTLSFITNNCSYNTILELDKKIEEIKPAHLEHKFERILFNKNELYTGAA
                     ISTGETVTIYPYVPRNLESFGEIAICSGNDRALEKVTLYPKK"
     gene            83013..83795
                     /locus_tag="PC471_000096"
     CDS             83013..83795
                     /locus_tag="PC471_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009900142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDF2787248.1"
                     /translation="MTEQQYFTLVTDIGKAAIANASITGEKVDFAKIKVGDGGGSSYT
                     PNESQTALKNVVWESTLEHAQGDKDNPNWVVIQKFIPGDVGGFEIREVGLFDSKDQLL
                     AISSYPTTYKPTADSGTVKELLIKVILVVSNVANINLKVDPTVILATLKDIQELDTKI
                     DTTKTELTSNIETAKTELNTKIGDTTQLTTTDKTNIVSALNEVKTSVDSIETTADKTS
                     IKDTDNLFESDNVEGALKEVMQEVKGNRSSIISTINSNLIPM"
     gene            84391..84879
                     /locus_tag="PC471_000097"
                     /pseudo
     CDS             84391..84879
                     /locus_tag="PC471_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893144.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            85281..85628
                     /locus_tag="PC471_000098"
     CDS             85281..85628
                     /locus_tag="PC471_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2787249.1"
                     /translation="MNRNNRIIYDQTGKIWLQTGEAIGDIQELSEITELNFIDVEFGS
                     IDYSKQYIESINPVTKELNIKNIEVVLTDEQKRLEVLEKELNMLKEENKNRDSEIVNT
                     AFEVGNIKLNNNL"
     gene            85640..85810
                     /locus_tag="PC471_000099"
     CDS             85640..85810
                     /locus_tag="PC471_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2787250.1"
                     /translation="MYNLLKLMIEQKNYSTKEDLQHKMDVFYAVNRITEEQYLELTGL
                     LNKEETPVEPTI"
     gene            85840..86121
                     /locus_tag="PC471_000100"
                     /pseudo
     CDS             85840..86121
                     /locus_tag="PC471_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889434.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            86429..86896
                     /locus_tag="PC471_000101"
     CDS             86429..86896
                     /locus_tag="PC471_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HDF2787251.1"
                     /translation="MSKSYFNTKLGGSMNVTIVFLATNIFIKLVILAIAFDTLLGCLR
                     AIKTHQFNSSFGINGGIRKVAMIACIFFLAVVDILTKFNFLFMLPQDWVDFLRLNHLG
                     ISEFFSILFILYESVSILKNMYLCGLPVPKRLKEKIGSLLDTMTDELNVKGGK"
     gene            86900..87730
                     /locus_tag="PC471_000102"
     CDS             86900..87730
                     /locus_tag="PC471_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003415961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HDF2787252.1"
                     /translation="MKICITVGHSILKSGACTSADGVVNEYQYNKSLAPVLADIFRKE
                     GHKVDVIICPEKQFKTKSEEKSYKIPRVNAGGYDLLIELHLNASDRQGKGSEVLYYSN
                     KGLEYATRICKKLGTVFKNRGAKLDKSLYILNSSKPTAILIESFFCDNKEDYEKAKKL
                     GYEGMAKLIVEGVLNKSIESNEVKQMYKHTIVYSGDDRVSADILGLYYKRAKESYLVI
                     DIKDYKPHRTQNLYVIGGVTCNKMKEMSKTTGEKFTQLYGNDVWSTMDKVIEFVKEKL
                     "
     gene            87802..88908
                     /locus_tag="PC471_000103"
     CDS             87802..88908
                     /locus_tag="PC471_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HDF2787253.1"
                     /translation="MLTFLDLFAGIGGFRLAMERAGHKCVGHCEYDKFANLSYKAMHN
                     PKEDEWFEKDIREIRAENIPKADVWCFGFPCQDISIAGNKLGFKGNRSSLFFSVTKLI
                     RELKEEDRPKYLLIENVKNLLSVNRGFDFAKLLIELDEIGYNAEWQNLNSKNFRVPQN
                     RDRIYIVGHLRRRSTREVFPITGTSRKTTLEQLNNPNHSSNRIYNSNGLSKCLLSKGG
                     NNTGLYRVGNINPSGNGAGGQVYSSEGFSPTLTTGKGESPKILIKENTKKGYIKASIG
                     DGIRLDHIEGKTGRGRVQHKKSCTLTTASNAGVIGKKFRVRRLTPKECFRLQGFPDDY
                     YDRAASVCSETQLYKQAGNSVTVDVVEEIAKKLK"
     gene            89046..89381
                     /locus_tag="PC471_000104"
                     /pseudo
     CDS             89046..89381
                     /locus_tag="PC471_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894425.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
     gene            complement(89392..89907)
                     /locus_tag="PC471_000105"
     CDS             complement(89392..89907)
                     /locus_tag="PC471_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009899650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2787254.1"
                     /translation="MTWSDLKEKNINEVVDCINNKLSEFESLKKVGDELGANESTIRK
                     WLNKKGYKRTGNKFVEDNKSYDDKYHIDSISSKIDDDIKYLSSEIDNIKNVLEWFKTK
                     DDSYHICVIKENDISIDLPDQSIKRTTVRINNTVWEMFNKFADENKHYDKHDLLSQLL
                     LESLLKYVKSR"
     gene            complement(89990..90895)
                     /locus_tag="PC471_000106"
     CDS             complement(89990..90895)
                     /locus_tag="PC471_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParM/StbA family protein"
                     /protein_id="HDF2787255.1"
                     /translation="MSKLGIDIGNYAVKTSTDDIFESKVTEVKNFGSDSDSIKIGNKT
                     YYLGEGDEEINIVKYEKENFLPLLLGAICRNTDDEIVDLGLGLPVKQFAGLRKNLIEK
                     LQGKEYHVEFTRGNETTKRDITIRSIQTFPEGVTGYLYYAKDIVDQIAGRDVVLVDIG
                     GKTTDIALVQGNKATDPYSINVGTINIYDAIKKSLEMDERFLGKVEIKREKIQDYINK
                     GFYLNGEKQDIKKNIDASISLFKEIYNELKLNYPVSTAAVVVMGGGAKLLGEAFKKNI
                     PGIIVMSDVDKHVFANAKGYKKMMK"
     gene            complement(90915..91118)
                     /locus_tag="PC471_000107"
     CDS             complement(90915..91118)
                     /locus_tag="PC471_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2787256.1"
                     /translation="MSRIVLTFKNNDKEKAIEKFLDEKLSATAYLKELVWEKMQEKKD
                     NAVVEQKKEIEDPANNFDFGSLE"
BASE COUNT        33467 a        13013 c        12802 g        31865 t
ORIGIN      
        1 attcaattaa cttctttgtg cttgttattt tgttacttct tgttgagcac atttattata
       61 ataacatctt ctcataatat ggtcaactat ttttttcatt tttttttaaa tttattgatt
      121 attcaccctt tgtcatacta ttgtgacctt atttttaaag atataactat tattaatagt
      181 aatttccaat tataataata ctatatgcaa ttgtaattaa tcatataatt attattatat
      241 gcaattatta ttaatttaga aaggagcttc aaaatgttta gaaaagtatt gcattattta
      301 acactacttt ctatttctat tttttttatt gttggatgtt caaattcaca aaacaatcaa
      361 aacgaaaatc aaaataagga aacacagtta caagaagata aggaaaaaat agatagtggt
      421 aaagatactt ccaatgtaat agtttcagac ggaacagata aaccatcaaa agccactact
      481 aataatgata ataataaatt agatgtatct tctttagata acactactct tgattggttt
      541 tatataccaa acaataagca taaaacacca gaagtaaata cggacattga atttaaattt
      601 tctgactatg atgccctata caatggacct acaaaagatg gtcaaaaaac actatatctt
      661 acatttgatg aaggttatga aaatggatac actacaaaaa tactcgacac tctaaaacaa
      721 aatcaggtta aagcagtttt ttttgttaca gctccatata taaaagaaaa taaagattta
      781 gtaaaaagaa tggtttctga gggacatata gttggaaatc atagtaaaac ccatccatct
      841 atgccaacaa aaacaagcaa tctaaaaaac tttaatgacg aattatatga tgttgaaaag
      901 ttatataaag atgtaactgg gaaagatatg gttaaatttt ttagacctcc tatgggaaaa
      961 tactcagaaa aaagcttggc aatgactaaa aatttaggat acaagactgt attttggagt
     1021 tttgcataca gagactggga tactgataaa caaccatcac atgaagaagc aacgcaaaaa
     1081 ataatggata atctacatga tggttctatc ttgttactac atgcagtttc caaaactagt
     1141 acagaaattt taaatgattt tatatcaaat gcaagaaaac taggatatga atttgaactc
     1201 ttggagtatt aatatttata attacatcta aagtctacaa actaatatag ttaatataat
     1261 ataggttata aaataataaa ttaaaattag aatttttgtt tggtgatagt ataggtaaaa
     1321 acttgtgata gcttatatat gttggtggga cagggcaata aaacatcaaa aaatttggca
     1381 agatattaaa tatagataac agtattttag atgttatatt tagatgttat gtatagtata
     1441 atgaaaaata ttattttatt aaagaatact aataaaaata tcaataaaaa tagatgtatg
     1501 gcttagaatt agtaaaacaa ttataataaa ttatagctac acatctattt ttaatttata
     1561 caattaaaga tttattcgat ttattaaata ataaacattg ctactatggt tgttacaacc
     1621 aatccggtta taactgggac aaagtttcgc tttgccaatt ctattggact aactccacaa
     1681 attgccgctg ctgaaataag accccaagga actagacagc ctccacctac ccatatacca
     1741 ctgatttgtc ctaatgcagc tagtgcacca acacttgcat ttatagcagt tccaaataca
     1801 gaggcaagtg aaccaactaa tgaaatccca gaaaatccag aaccatcaag cccagttatt
     1861 gctcccacta cagtttctat acctgatacg gcaaatttat taagtggtat tgtttgagaa
     1921 agagctatgc ctatatctga taataaacct tgtgaacctg gtgctaatac ttttccaaat
     1981 acacctgtca atggtgcaac ttcacccata tagaaaaatg ctgctattgg tataattgct
     2041 ccaaatattt ttatcccaaa cacaaaacca tcaattatat tctcacatac attttcaaga
     2101 ctatctttgc caaaatttat aacatttata attattatta aaaatactgc tgttccaccc
     2161 aaaagagctg atgcatctcc tccttttagg tcaaatatat acatagcaat tatatcagca
     2221 agaaatccca gagctactaa aatagttgct attttagatt ttccactgaa ctcttctact
     2281 tcttcacttt caaaaccttt tagttcttct ctaaacattc ctttgttaat atctcttttt
     2341 aatgtataaa atgccataga tatggttacg attcccatca cccaaaacaa aatcatacca
     2401 tcatttataa catatgatac tgcaacacct gctgcaccag ctgttatact tggagctcct
     2461 tgaataacaa agtctgtaga caaggcaaga ccatgaccaa ataaatttaa tgctactgca
     2521 acaccaattg ctggtagccc agctctcata gcaacaggta aaaatacagc tccaactaag
     2581 gcaacagctg gagttggcca gaaaaaccaa gaaagaacta acataacaaa tccagttaca
     2641 aaaaatgctg tattagaatt ttttataact ctcgaaaatg gtctcaccat atactgtata
     2701 gcattattgg cttctagggc tttagataat gcaaccataa tagctataat caagataatt
     2761 ggacccaact catttaaaga tataataaaa gagttaaaca ctgctgttat agcaccaaaa
     2821 atgttttttt caaagaacaa ccccataaaa aaaacaccta atatacatgg tactattgta
     2881 tcctttttca taatcatagt aattaatata attaccataa atagaatgta aatataatgt
     2941 gctcctgtca aaaccatatt attatgtccc ctttttttaa gataataata ttatatggtc
     3001 ttgtaagtaa acttgttact tgcctagagg tacacaatat tgaacatccc ataactaaag
     3061 tcacatgctt cctgcttcat agaattttac ataagcaatt acacaatata taatagcaat
     3121 tacacaatat ataatttaaa tttttatgat tcttttgctg gaatagatat ttcaatagca
     3181 aattctttat catctgaatt tacatttatg cttccattat gatatgcaac tattttctca
     3241 caattcttta taccaattcc aaaactttct acattcttag aaaaattact tactctattt
     3301 ttttgtataa ttagtaattt agaatcctct actttaatta aaaatactat aggcttatct
     3361 ttcctagcat attttattat attagaacat aaattatcaa atatccttct aaattgtgta
     3421 acatctacat aaatatgata ttggttataa agttcatttt ctattgttat atgaaatcct
     3481 ttctcctcca atgtatatga actatctaaa agtaattgaa aaataaattc attagcattt
     3541 attttttcat acccttttga aacttcctct ccagaattaa caaaatgcgt aaaaaggtca
     3601 tctgttaaat cttttatttg atatgcttta gaccttacat tttttatgta ttgttccaat
     3661 tgctcaggac tattgtattt atggtcttta atcaaatcta aataacaaat tactgacgtt
     3721 aatggagttc taacatcatg tgatattgaa gttactatat ctttattttt attcttcatc
     3781 tccctttctt ttataagttg attctccaat gtatgactca tctcattaat actagatgca
     3841 agtgatgata attcatcatt accttttact ggtattctat actttaaact gccactctct
     3901 actatcttta tacccatttc tattttcctt aggtacttta tctttctact aaacaacagt
     3961 aaaaatgtta taataaatgt aagtgccata aatgtaatag aaattgcttc tataaaattt
     4021 tgaaatctaa atctaaataa agctactaca actatattag ctttaatatc agaaaattgt
     4081 atctcatagt ttctagccat gggactgcta taaaactcat tttcctcact cttatcaggt
     4141 acttcattag gatagtataa tgtatcataa aaaaccttat ttcctttata tatcttcata
     4201 gaaacaaacc aattatcact attccatttt gctatatcat ctccatcatc tgctcttata
     4261 ccattgttat tcacataagt ttgaaaatta tccataagtt gatttacttc tcttttaaaa
     4321 tatactttcc cttctgatac atcttttact atagattcaa cagcagagta aaatggtata
     4381 tttaaaatga aagccactgc agctgagatt actacatata tcaatagtat aactcccaac
     4441 ttagatggct taattttgtt aatcaatata ataccccttt ccccatgcgg tttttatata
     4501 ttgtggattt ttttggtctt tttctaactt ttttcttaaa tttctaatat gaaccattat
     4561 tgtattattt gctgtataaa aatactgctc atcccaaata gtttcatata aattttcaga
     4621 cgaaaaaact ttttttctat ttttcaacat aagaattaat atactatatt ctatagttgt
     4681 caaaaacaca ggttctccat ttagtgtaac atcttgagta tctaaattga cacataactc
     4741 ttttgttttt aatatatttg gctctctttc cttttccttt ccctggtata tataataacg
     4801 tctaagtaga gacttaactc ttgaaattag ttcagaatat gaaaaaggct ttgataaata
     4861 gtcatctcct cctgctgaaa atccaataac tttatcagag tcttgagttt ttgcagttaa
     4921 aaataatatt ggtgctgtcg ttttttctct aatttcatta catgcaacaa atcctgtttt
     4981 tacaggcatt acaacatcca aaataatcaa atcaatcgta ctatctacta agtttacagc
     5041 ttcttctcca ttcgttgcca taataacttc ataaccttca ccagttaaaa gtatttctac
     5101 gatttcccta atctcaggat tatcatcagc tactaaaatt ttttttggaa tctctttatt
     5161 catcaccaag ctcctttttt tgctatattt cttaaatatt tcaaactata taaaatatta
     5221 tactttatta gcaaaagctt agcaatgtca cacgttttaa tagtttctac tcctgtaaat
     5281 atgtttaaag cttaattaaa atatacacac aaattgacat attaataaaa ataatctata
     5341 taatattatc ttacataagc aacttttttc actgctttta ctttttcaga agattttaca
     5401 tgtactacta tacatatcaa tccacttata gctacaaatg cataaaaact tattccccta
     5461 ctaagtaaca ttgccggaac taataagtga ccagggaaaa atatcttaaa taatgtcata
     5521 aaactatttt cagaagctcc aacagcccct ggcaatggta atgaagaaac agcaatatta
     5581 agcaaggatt gtatcgctaa tatctcaaat acagtatatg ttgatagatg aaaagctttg
     5641 tatacaaaga aaggaactaa aaacatagct gttatttgta caatagttat gactaaaatt
     5701 tggactacaa gttttggatt ctgctttata tattgagcac ctttaacata ctcttcaatt
     5761 tgagatatta taacttttct tgaactttca acatctttta ttattcttaa cttacctaat
     5821 aaatttgtta ttgatattat aaaattgttt acaagttttt ttgaaaatat aattgcaatt
     5881 actccaatga caagagcaac atttgttatt acaccatata ttagtaaaat gttcatacca
     5941 cttacattat ttaatataaa ctcacgttcc ataatgaaca ttattccact atatgccagt
     6001 ataactactt ggtgaataac aacaatgact aatagtataa gtgaagaata tgatatattt
     6061 atatcgtctt ttttcatata ataaacttgt gctggttgac cacctgatgc tgaaggtgta
     6121 atagaactaa aataaaatcc aataaaagca tatcccatac attttaaaag agtagtcttt
     6181 tggtttaaag ctttcatacc taagtatata tttatacctt cacaggcaac atatataaac
     6241 atcattgcaa atccaattag tataaacata gggcttgcac cttttaatga ttttatcaat
     6301 gattcaactg actgccctct aaaaacaaaa tatcctgtta tgcccattag taaaactaaa
     6361 aaagctacac tacctaggca tttatttttc ttttcagttt tacttatcat aactacatcc
     6421 tttctggtgt tacgaacttt aaaccactgg ctttaagttt tggaatagct atctttaaag
     6481 cttcaatagt acgctcaggt gcacctactg caccacctga attttcacct gcatcatgta
     6541 aacaaattat agaattttct tttgttctac tcattaactt gtttataata atgtctactg
     6601 tactatcttt ctcccaatct tctgccatta catcccacaa agtcattttt agattatatt
     6661 ttttaacaaa tgaatttgaa aatatattag tatgacccca tggtggacga tagtagttta
     6721 catctacacc taaatttttc attatatttg tactttcaat aaaatctttt ttaacataac
     6781 tataactata aagccatgca tttctgtgtt ccaatgaatg taaaccaact atatgatttt
     6841 ctctcaacat tctttttata atttcaggct ctttttcagc attctttgct actacaaaaa
     6901 acatagcttg tatatcattt tcttttaaca catctagtaa tttttctgta tatctcttat
     6961 ctggaccatc atcaaatgta agtgcaattt cattttctcc agccatattt tttaatacct
     7021 ctttatttaa tagtttatta tagtatgtag gtataattga atggactaaa aaaatcaatg
     7081 caacaacgat aattaaacct actacataca ttctctacac gctccttgtt catcaagtga
     7141 aaataaaata ttctctaagg tattattatc aaattgtttt tttaattctt tcatgttact
     7201 gctcatttct ttaagagtag catcatcata aattaaatct tttatctcat ctacatagta
     7261 ttttttattt ttaccaagta ttctcccaat ttcattattt aatataaaac tagcattatc
     7321 tatctcttgt tgtaaaaatg gattaaatgc caatattgga agttctgaat aaattgtttc
     7381 aaatagtgtt attccacctg gtttagaaat aataaggtca gaatctttca tatatttata
     7441 tacttcattg gtgtaaccta ctacctctat attttcatat ctaccatata gtttatagta
     7501 cattttttta ttattaccag ttattacagt agtttttact ccttctaaac tatttaattc
     7561 tttgtaaaat tgttctgatt taggcagtaa tcctaatcct ccacccatta taagaatatt
     7621 ttttttagtt gaatgattta catgttctat tcttttaaat tcttttttta ctggaatacc
     7681 attaacttta atcttagatt catctattcc tttaaaaaca agttcttcct tagtagattt
     7741 actagctact aaataacagt ctgtattttt actaatccat tctgagtgac tacttatatc
     7801 agttatacat gttattaaag gaagatttga gttatatttt tccttatatc ttgatactaa
     7861 ttgagaacaa aatggaaaag ttgaaataac tactgttggt tgtacttcat gaagtaatgt
     7921 atctaattta tttaaaaaat aatctgaaaa agtaaatttt atatctttct ttccattttc
     7981 agcacattta taaaataaat tataaagact acttccccga tttacaagaa tagaaaaagt
     8041 cttgtacatt ttatctgaat agttaggcat aatgtactca aatatatctt taattattat
     8101 ttctgaatta tcaaattttg cttttatatc ttcacttaaa gaatttgatg ctgagtaatg
     8161 acccatacca aattttcctg taagaattaa taccttcata agattccctc ctcgttaaag
     8221 cttttctttt catatgatta ctatacttat aagtatagac tttaaatctt tagaaaaacc
     8281 tagtataatt cttaagaaat cttaaagttt taattaaaaa atacaaagag accatctaaa
     8341 aatagagata accatatttt ttattattta aataacacaa atatgttcga ttccatacaa
     8401 aaaagtgcct cataaactaa aaagttcatt ttgagacacc ttttttatat ctaactatat
     8461 tcaaatacat acacaatcat tgtaattatt taaatgtatt ttatttagtt gttctttata
     8521 attaatccat aatattatat tcttcatata aaggatattt ttcagttaaa ccaacaacca
     8581 atgttctagc cttagattct tcatcataag ttaaacatag gtctatagcc tcagcgatta
     8641 ttgccatatc ttcttctttc atacctctag tagttacagc tggagtacca agtcttattc
     8701 cacttgtaat aagagcacca tttgggtcaa atggaattgt atttttatta gctgttatat
     8761 aagcatcatc caatctcttt tctgctgctt taccagttat atttttatta gtcaaatcta
     8821 atagtattaa atgattatct gtacctccag aaatcaattt aaaatctctt ttaataagtt
     8881 cttcagctaa ggcttttgca tttttagcaa cttgtacctg atattcttta aactcatcac
     8941 ttaaagcttc tttaaagcaa actgccttag aagcaattat atgttcaaga ggtccacctt
     9001 gaataccagg gaatattgct ttgtctatgt ctttagcata tttttcttta caaagaataa
     9061 caccccctcg aggtcctctt aaagttttgt gagttgttgt tgtaacaaaa tcagccactt
     9121 cacatggatt ttggtgaaga ccagcagcca caagacctgc tatatgagcc atatctacca
     9181 taagcaatgc tccaacctca tcagctatct ctctaaactt tttaaaatct atttctctag
     9241 gatatgcact tgctcctgca actatcattt taggttttac ttcatgtgct atctttctaa
     9301 cttcatcaaa atctataagt ccactgtctt tatctatacc atattcataa aatttataat
     9361 attgaccaga aatattaact ggagcaccat gagttaaatg tccaccttga gataaattca
     9421 ttcccataac aacatcacct ggttttaaca tagcaaaata tacaccaata tttgcatttg
     9481 ctccagagtg aggttgaaca ttagcatgtt ctgctccaaa tatttttttg attctatcta
     9541 ttgcaagagt ttcaacttca tctatgtatt cacatccacc ataatatctt ttgcctgagt
     9601 aaccttctgc atacttattt gtaagatgac ttcccatagt ttccattact ggaactgata
     9661 ctatgttttc tgatgctatt aattcaatat ttctttgttg tctttttaac tctcttttca
     9721 tactttcata aatctcaggg tccgcttttt tcaagttctc aaacatatta ctcctcctag
     9781 ttattattta aattttattt ttacagacat ttcataattt tcgtttttat caatatgata
     9841 atttattatt atagtccttt accaattaat agtcaagtgt tttgccattt ttccatattt
     9901 aacatagtta ctttctttat ttttaagtta aaatataata aataatattg tctaattctt
     9961 ttaattggat ataatttaaa tgtgactatt ttgaaacaaa aaaaagataa tatatttgta
    10021 tagatattaa ggaatattac ttaaaaattt gatataattt caatattacg ttttttaatt
    10081 tggaggtgta tttatgaatc aaaatgagga acttgaatat aagaaaaata ttctaaaact
    10141 agctctattt gtaggggaac tgatgataaa aaatggagct gaaacatcta gggttgaaga
    10201 cagtgtcctt agaatatgca ggtcaagagg gttttatcat gtaaatattt ttacaactcc
    10261 tacagtaata attatttcag atgagaaatt tgatggcttt tcgtttatga aaacgattca
    10321 atcaagaggt ataaacttaa ataagatttc tttattaaac agtttttctc gagaatttgt
    10381 tagtgacaaa gagtatgata ttgataatgc cattgcacgt ctttatgaaa tccaagatgt
    10441 aaaaccttat cctttatggt tattttactc ttgtacagga attgcatctg cttgttttgc
    10501 ttgtttgctt ggagggaatt atgtattaaa ttttatactt acgtttataa tagcaatctt
    10561 tgctgcaatt acttatgata aaacgatgaa gataagtgca atatctgcat tttcctgttt
    10621 agtatcttct tttttaattg cactttcagg agttctacta gttgaaatag gcattttaga
    10681 tgaccctaaa atgcttatag tcggcgctat aatgccacta ctcccaggag ttgcttttat
    10741 aaaagctatt agagatttaa tatcagggaa tttaatatca ggaatttctc gtgcttttga
    10801 tgctgctatg attattattg caattgcttc tggagttggt tttgtccttg atacatggta
    10861 tagaatagga ggtcctttct aatgactgat atgtctatga ttttgcattt tttattttca
    10921 ggtgttgcaa ctgctggatt tgctgttttc tttaatgctc cactatatct acttcttcca
    10981 gctggagtaa ctggcggaat tggttggatt gtatatgttt atttatttaa ttttactaca
    11041 aatgcagttt ttgcaggttt tatcgctgct gctttggtct cagcgtgcag tgagacttta
    11101 gcaagaaaac taaagcaacc agctattgtg ttcgtaattc caggtattct tcctctaata
    11161 ccaggaattg ggctatataa tacaatgttg tctttgattc agaaaaatta ttctttggct
    11221 atgtcaaaag gtacagatgc gctatttcta agtgctgcaa tagcacttgg tgtattagtt
    11281 gtaacttctt ttgtaagaac attgaatctt ttaaaaatca gaaaaaactt tattccttac
    11341 agaaaatcaa aataaaaata taaaaatagc aatgtactta aactctacaa atacgtattg
    11401 agtatttatt taaaaagtac attgctattt tttaattata attatcttaa aggtcaatta
    11461 atattcttgt tattactggt ataaattctc tactgtctct aaattttttg tcattcttct
    11521 ttaagtataa ataacagatt attgtaagac aaattccaac taatccactt atggcttcaa
    11581 ctccagttgt cattccaatt gcccccaata tataataagt ccctattgtt cctaaaagaa
    11641 gtgctataag aggtattata taagccatta cagctgcttt taatacattc atattatcca
    11701 tactcacttc tacatggtca ccaacttttg caccaattgt gttgtctact tctactaata
    11761 tttcttttga ctctgctgaa tttgtagtag cacattttcc acaagcagca caagctgaat
    11821 gtctttgcat ttttaacttt gcagttctat catcaacaat ttccataata aatccttgtt
    11881 gattcataac taaccgcctc cttattttgt aaatttattt atagtatcca taagctcttt
    11941 tatcatctct tcagttttat cagcatcatc atgtttaata ctttctgcta cacatccttt
    12001 tatatgattc tctaagataa ttgtgcctac tctatttata gcagacctta tagcagatat
    12061 ttgaaccaat atatcaacac aataacgttc ttgttccacc atcttttgta tacccttaac
    12121 ttgaccttct attcttttta atctttttat gagagcttcc ctctcattgt ttggtgctaa
    12181 ttttctttct tccataaact cacctcatac tctaatgatg tcttatacaa ttatgctcta
    12241 cttaccaact tgtttaaaat ataatacagt tattattact ataaatgcca aaattgatat
    12301 tgcaatagac ctactttgat tcttagtttg ttccatactt aactttccat tcattttaaa
    12361 tcctgccaat gggaataaaa atataaccag tgcatcaatt ccaagtacaa cttgggctgc
    12421 aatagtccat ttactcaaaa agcagaatat tccgcccaca cctaaaagaa cctctaatat
    12481 atttataaac ttatatgtag gtttttttgc tatatattct tcaatttttt ccttacattc
    12541 tgcacgacat ggatatatat tcatattgtc atcatatttt atagctaatt tattatttgg
    12601 tgactttata tccctattac agtaaagaca ttttttcatt ttatattctc ctctagtctt
    12661 ccttaaaaaa ttttgcctaa attattatac gctctaatac tattatacca aatagataca
    12721 aaaaacaaaa ataaaaggaa taattttagc tacttctttt acttcaatgt gtagtctata
    12781 ataaatttat aataataatt aaataatatt atttaatcta atttaattat tatgtaatct
    12841 aaaataagtc aaatttgtat gagaggttaa aatgaaacaa atggaattag ctattgtaag
    12901 tttaaaaaaa gacgctggag aaatttatga aaatcaaata aggcaattct taggtgataa
    12961 tctaaagata aatttatact catttgaaga gggaaattta aagtttttta aagaaaaact
    13021 tatactccta tctgcttatt taaaatatga tgaaatagta aaattatctc actatgatgc
    13081 tcaaatcatt gttcctaagt tgacatttga aaagaacagt attgatatga tatccaaatt
    13141 agaaaaagat aaaattattt atgtatataa cttaagtaaa gatatggcaa tagaaacaat
    13201 ttctttaata catagattag gaatagacaa tataaatatc ctgccctgct atccagagat
    13261 tgagtttact cccacagatg ctgtaatcct aactcctggc gaaaaaatct tgcctaagtt
    13321 taaaaattgt gaagttatag atttaaaata cagaataata gatttaagtt gtattgttga
    13381 aattgctaca aaaaccaaat taaaacatct aataaaagat gacctaataa aaaaatacgt
    13441 agaaaaaata attcctacaa gttatagtac aggtgagtta ttgttagatg caaataagtt
    13501 tgaaagacaa tttgatttgt tgctatctat aattgatgat ggaattatat gcacaaataa
    13561 cgatggaata attcaattct ataatcatat ggctagaaaa attttgtcta taaatgcaaa
    13621 tgaaatgatt gactcatttg ttggtgattg cataaaagac atcaattttc aaagcatatt
    13681 gaccaataaa actccatttt ttgagaaatt gattaaaata aatcatattg atataaattt
    13741 agagattaaa catatacaat taaatgtatt tgatggattt atactaaaga tgacaaagtt
    13801 ttctcaatta gaaaaaaagc aagctaaatt aagagcacaa ctagtaaact ctggtaatat
    13861 cagtaaatac acatttgatg acatattagg ttctagtata caaactatta atacaaaaaa
    13921 aattgctaat aaaatggctc aatcaaattc atctatcctt ataattggtg aaagtggtac
    13981 tggtaaagaa ttatttgctc aatccattca tagtgcttct aggagaaaag atggtccttt
    14041 tgtcgcagta aattgtagta cttttcaaga aaatctactg caaagtgaat tatttggcta
    14101 tgatgaaggg gcttttactg gtgctaaaaa aggtgggaaa attggtttat ttgaattagc
    14161 caacaatgga actatatttt tagacgaaat tggagaaatg gatttaaatt ctcaatctaa
    14221 attacttaga gtcatacaag aaaaacaagt acgacgtatt ggttctaata atgtcattga
    14281 cattgatgta agaataattg cagctactaa tagaaattta aaagaacttg tatctaaaaa
    14341 tatgtttaga agagatttat attttaggtt gaatgttctt ccccttaaaa tacatccact
    14401 tagggaaaga cctgctgata tatttgaaat ttttggttca ttaaaatatg atattccatg
    14461 taattttatt ctaagtgagg aagtaaaaga aatctttaaa atgtatagat gggaaggaaa
    14521 tgtaagagaa ttaagaaact taggagaata cttctgttac ttggaaaaag atattataga
    14581 aatatgtgat ttacctgaat atatattaga tactatagat tcaaattata gtagaaccgt
    14641 ttgtaataaa gtaagtgata atataaaaaa gtatcaattc aacattggca aagacaaaaa
    14701 catcatgaaa tacgactata attttaaaag gtcactagat gaatacatat ttatattgga
    14761 taatttaaaa aaggcttatg atttaaaaga aagaataggt agaaaaagct tatgtaaaat
    14821 agctttagag gaaaatagat ttttgacaga acaacagatt agaaatatgc ttttagaact
    14881 acaaaacttt gggcttgtag acatacttgt tggtcgtgga ggttcaatta taacatcaaa
    14941 aggagtagaa tttctaaaaa atataaatag gtctaacaag ttaaacagtt aaacctattt
    15001 aacctattta attacatttt catatattta aaaatttatt agaaataacc ttttccacaa
    15061 agttttaagt ataattaata tttttatctt atctatgctc tgaaattact taatattaaa
    15121 tattaacttg tgaataaata ttttttatta tcaatattgg cacgttgttt gcttaatatt
    15181 attaacataa aattaaaatt actggaggta tttacaatga attataattt taatgagatt
    15241 gttgatagaa gcaataattt ttcatctaaa tggtcagaaa tggaaaaaaa gtatggaact
    15301 aatgatttac ttcctatgtg ggtggctgac atggatttta aggctgcacc ttgtataatt
    15361 gattccttaa aaaatagact agaacaagag atttatggct acacaacaag accagattct
    15421 tacaatgaat ctatagtaaa ctggctagat aggagacata actggaaaat aaaaagtgag
    15481 tggttaattt atagtccagg tgttatccca gctataagtc ttttaataaa tgaactcaca
    15541 aaagcaaatg ataaaataat gatacaagaa ccagtttaca gcccttttaa tagtgttgta
    15601 aaaaataaca atagagaact aattataagc ccattacaaa aactagaaaa tggaaactac
    15661 ataatggatt atgaagacat tgaaaacaag attaaagatg taaaattatt catactttgt
    15721 aatcctcata atccagttgg tagagtatgg acaaaagatg aattaaaaaa actaggagat
    15781 atttgtctta agcataatgt aaaaatcata tctgatgaaa tacatagtga cattatatta
    15841 aaaaaacata agcatattcc tatggcatct atttctaaag aatttgaaaa aaatacaatt
    15901 acttgtatgg ctccaactaa aacttttaat atagctggtc ttcaatcatc atatgtaata
    15961 ctacctgatg aaaaagacta taaattgtta gatgatgcct ttaccagaat tgatataaag
    16021 agaaataatt gctttagctt agttgcaact gaagcttcat ataataatgg tgaatcttgg
    16081 ctagaatcat ttttagaata tttagaatct aatatagatt ttgctataaa atatataaat
    16141 gaaaatatgc caaaacttaa agttagaaaa ccagaaggta cttatttatt atgggtagac
    16201 tttagtgcac ttggattaag tgatgaggaa ctagaaagta tacttgtaca aaaaggtaaa
    16261 gttgctctaa atcaaggaaa cagctttggc attggaggaa gtgggtatca aagaataaac
    16321 ttagcttgtc caagaagtat gcttgaagaa gctcttataa gaataaaaaa tgcaattaac
    16381 taaaaatatt taaggagaat ttgcactatg aaaatgaata cagaaagaaa aataccttca
    16441 tttagatatt ccttgttagt aatgcttgca ataatagctc taacatcagt tggtatagtt
    16501 gtatttaatg cttctataac tacaatgttt ttactttcat ggcttatagt agttcctgca
    16561 gctatgaaac ttggttatac taatgatgaa atagaagctt ttggatttga agttggtaaa
    16621 gatgcctttc aatcaaactt aattatactt tctgttggtg tactaatagc agcttggata
    16681 gctgctggta caataccaac agtagtatat agtggactaa caataataac ccctaaatat
    16741 tttctgctta caacattaat cgtatgttca ttaacttctg ttgctactgg tacatcttgg
    16801 gggactcttg gaacttctgg tatagcaatg atgagtattg gtactagtat gggtattcct
    16861 cttggtttaa cagctggtgc agtcatttct ggtgctttct ttggagataa gatgtcacct
    16921 ctatcagatt ctacaaactt agctgcagca gtttgtaaga cagatgtaat tacacatatg
    16981 aagcatatgt tttataccac aggacctgca tacgtaatat gtatagttct gtatacagta
    17041 attggattta agtattcaaa taatactata gactatgttc aaataaatca aatcaaggat
    17101 gtattaaatt caaactttca tattggttta gtagcaatga ttccaataat atttttatta
    17161 ttacttttat tgctacaaaa gccacccatt atatctatac taagttcagc tatcatggga
    17221 cttattatag cagtatttca agaaggagaa aaaataggtg atgttttaaa ttacatgcta
    17281 tcaggattta ctatagatac tggatttgtc tatgccgaca aactattaaa tcgtggtgga
    17341 ataatgagta tggctgaaac cgttttacta gtatttgtag ttttcgttat tgctggtatt
    17401 ctccaaaaaa ctggattctt agaagtactg cttcaacctc ttataactaa aataggaaaa
    17461 tcaagaacta aattggttgg gtctactttt atagtaagtt actttgcaaa tgcatttagt
    17521 tcatcaatga tgttcacttc tgtatttgtc ggaactctaa tgtctccaat atacaaagaa
    17581 tttaaattaa aaccacaaaa tctatctaga attatagaag atacagctac acttggtggt
    17641 ccattaatac catggaattc aaatgctgtt ttctgtgctc agactcttgg tgtaagccca
    17701 ctagaattta ttccatattg tttcttaagt tggattacac ctataatttc atttatttat
    17761 ggtgtaactg gttttactat gcttacatac acagatgatg aaattgaaga gttagaaata
    17821 tttgatactg ctcagagaag agttgattga atatgaaatt tgaatactat tacttaattc
    17881 aggatattgc aggtatatta ttggcattta taggacttag aatgtctatt attggattta
    17941 ggatactttc tatgaagggg ctttctataa atacactttt aattgtaatt aaatactgct
    18001 tatttaccat agcaggactg aatttattaa tatctaagtt tggaattaga cactggattt
    18061 ggagtgtttg tatgttgata atatccataa taataaatcc caggattaag gtatcaaaat
    18121 agaaatagtt attagactta aatttttata aaaaaactgc tttacaaatt aaagcagttt
    18181 ttttattgtt acttataatt aaataactgg tgtatcagat aagtaatcat tacttacata
    18241 accatatcca ccattatact ttatttttac ccatccagta gcaaacattt ctattgtttc
    18301 tactttgctt ccatatgaaa ggtgacctat tttttcatct tctgtacttg gtcctttcct
    18361 aacatttaga ccatcttttt cttttacata ctttattttt atattttcat tactattatc
    18421 aggcttatta tttgtcaaat aagacccact aacatatcca ataccttcat tgtattttat
    18481 ttctacccat ccagttttta acacatctaa aacttctact tcatcaccat tatttagata
    18541 tcctataaca gaagcttcta ttgtagcatc atttcttata tttaccttat ctgtattagt
    18601 tacatacatt actctcttat tgttactatt tgcctgtaaa tcttttaatt ctttaaatgc
    18661 attttcattt cctccactac caacttgagt tatagaggta gcatttttat tcgaaaatac
    18721 actcttttgt gttgcactta attgttctcc tacgattagt acaggagaat tttcttttga
    18781 tgctagtact cctactgcta aagcgtctat aagttgattt tgatttttca ttccatcctt
    18841 agccacaaat acattgttta atttattaga tgtataaaac ttttctatta ctttaccatt
    18901 agtttcattt ctatctgctc cacctattct ttctgaatta ggtaaacttt gttttatttt
    18961 atctgataca gcattagtag agcctattat gtatgattta tttatattct tagattctat
    19021 aaaatcttta aagctagata taccagatgc atcagatact ggaagtataa ccattccatt
    19081 agtagcagct acagaagcta tactaactgc atctgcaaga cctgtgtacc catttactac
    19141 agctatttct gatatatctt ttaatccttc tattttttta gctatactca aagctgtttg
    19201 gtatctatca ctaccaccaa ctctatcagt agttaaatta ttagattcta actgtttttg
    19261 cacatcattt gatacagaac tagatagacc aattatataa acttttttaa ctcctaatct
    19321 ttttaattct ttctcagttt gtgaatttag attatttttt tctgtcaata atataggagc
    19381 attttttaat ttagcaaatg gtgttacagc taatgcatct acaatagatt cactatttac
    19441 aactacagcc tcatttgcac tactccatcc ttttttactt acttcaacag cagtttgata
    19501 tctatcctga cctactaaag tattctcaac tgttgttcta cttatttcat ttgcatatgc
    19561 tactggtgat aagcttaaaa ctagagataa gcacagcatt gatgatattg atttagttaa
    19621 tttcatcttg actcccccta aaattatagt atctaaatgt ttcctattta aaatagtata
    19681 acataataat ttaagtaaca tcattattat aaaatattat ttttcttatc taataagctt
    19741 ttattcttac ttgacattaa tactttgctc atcaaacgaa ttttttatat aactaaagtt
    19801 tgtaacaata tttccttagt taaccattta aagttacttt ttttaattag attatgtaca
    19861 aaatataaat aacaatttaa ataacttttt aatatattgt agtttattat ataaacttta
    19921 tattataatg tataatgtgg agttgattat aaaattgata taactcaagt catattaaaa
    19981 gaggtgtatt ttatactatg gatttattgc atcttaagta ttttcaaact gttgctagaa
    20041 tggagcacat aacaaaagca tcacacaagt taaatatagc acagccagct ttaagcaaaa
    20101 ctatatcttc cttagaaaaa gaacttggag ttcagttatt tgacagaaaa ggaagatata
    20161 ttgttttaaa tgagtatgga agattgtttc taaaaagagt agatagcatt ttagatttag
    20221 tagaaagttc aaaaaaggag ttacaagaca cttctttgga aaattcagga gaagtaaaga
    20281 tattatctcc tgcagcggct aatgtgttac catcactttt gagtaatttc agaaaactat
    20341 atcccaatat tacttttaat gtgtcacata ctttgccatc atcttataaa aaaagtgatt
    20401 ttgatttgta catatcttct tcatttacaa agctaaactc agaaaatagc ataactttaa
    20461 catgcgaaga aatactatta ggagtttcaa taaaccatcc tttatcatta aaggatgaag
    20521 tatatctaag cgaagtttca gatgaaaact ttatagttat aacgaagggt gagaattata
    20581 gagaggtaat tgatatcctg tgcgaatcag ctaatttcaa acccaaaata gcatttgagt
    20641 ctgattcacc atatacaatt tatgctctta taaaatcact tcaaggagtt ggatttatat
    20701 gtggaaaatc atggggatta tctcaagacc ctgagattaa gttgttacat atcaaagata
    20761 ttgaatttaa aagatattta aatctttcat ggtttagcga aaactatgaa tctaaagctg
    20821 tgttgttgtt caaaaacttt cttatcaact attttaaaaa tatttaaatc aaagttgata
    20881 atattaaaga ggatatatca aagtataata aatttaatct taatattgat acatcctctt
    20941 tttatcattt tatattgaac ttgtattata gtatagaatt atatgttata gctacttttt
    21001 ctgtctttca aataggggat atcttcccta tactttttta tttcatctaa atcaatctct
    21061 gataatacat atttttccct gtatgtatct aattgagtta ttacagtacc tctaggattg
    21121 catattttac tctttccaaa tagtattaaa ttagagtcat tacttactaa atttacacct
    21181 actgtatata cagtgttctc taaagctctt tgagatacat ttaaatccca catatcttca
    21241 tcttgtattc tccatgcaga tggtataaat attatttcag aaccttttaa tgtcaattct
    21301 ctggatactt ctggaaaacc tgcatcataa catatcatta caccaaatct accaaaatca
    21361 gcatcatata cctctacttt ttcccctcct ttaaagtata ctgcttctaa tgaccacaga
    21421 tgatttttac agtattctcc cataatctta ccttttctat caaatataac tgctgaatta
    21481 taaacatgtg tagacccttt ttctaaggtt ccatatggag atatcaggta tacattattt
    21541 cttttagcag cttctgacat tgattcctct atgtacttat tatgttctct tattaattct
    21601 aatgttttaa ctccacctaa agactctaaa ttatatcctg tagcaaatag ttctggtaaa
    21661 caaattatat ctgccccttg ttttcctaag tcatctatca tctctactgc tttttctata
    21721 ttttttttaa catttccaag tacactgtgt tgttgaatta tacctatttt caccttacga
    21781 cccatgtaat tgcccccttt atttatcttt gattccaaaa caattcatta atatataata
    21841 aataaccata ctcactacaa ttccatttat cggaggtatt aagaaattta tttttgaact
    21901 ataatacgac gctgcaaaac ctattaacca acttattact gcaatccaat ttattccttc
    21961 tctatgctta taagttcttc cttgtataaa ataatactca caccacataa ttcctgctat
    22021 tgcaggaact gcgattccca agaaagataa aaatgactga aatttaaata ttataccagt
    22081 agctgctatt attgtaccta ttagacctgc aactaaagtc aaaacatttc tttttacatt
    22141 tggaaaaata gtttttaatg ctaatccacc agaataagca tttgatacgt ttgtagacca
    22201 agatgcaagt attaaaataa caaacgctac ccatccaaaa cctaaattag ccaatacttc
    22261 tgttatatcc caagtaggtg ctcccataga cattacacca gctgcaactt gttggaataa
    22321 tgaaataatc cctagaccta aaaaactgat aatccaaatg tcttttactc gtctcgtata
    22381 tcttaactgg tcaggtgata taattgcacc aactataaca agacctacta caatattaag
    22441 accttcaacc atactcatag ttgtttgagg agtataatta taaatagctt caactccata
    22501 agtagaaaaa gctttaaaaa ctccccacac cattaatatc ataagtggtg gtataactaa
    22561 gtaattaaat ttagccatag ctttaaaacc atacagtgca aaaacaacca taaataatcc
    22621 aagtataact gtaactaatg ttaggtttac acttgggaat atcttttgta ctgatagtcc
    22681 tgcaacccct gcttgtacac caaaccatcc aaaactagtt ataccaagta atagagatat
    22741 aagatacttt cctcctatct ctccaaatgc ccccttagct aatacatacg tatttaaccc
    22801 ttctttgtaa cctatataac cttgtatcat aataattgca actaatatta agttccctat
    22861 aattgatgta ataatcatat tagaaagagt cattcctaaa attaaagttc ccccaaccat
    22921 tagttcagat atagctatgt tagaaccaag catattaaac aatggagcta accatccaac
    22981 tctttcctct tttggaactg gatttaaaac taattcttct ctctcttgca atattatccc
    23041 tccctttctt tataaaagaa taatttctac ttttttctac ttttttttca ctattaatag
    23101 aatttttctt tatataattg taattctatt ctacaaaaaa gtataatatc tagtttgtta
    23161 tcaatttgat ataatttaag ttataaatag ataaatagtc caaatttatt attttaattt
    23221 aatctattaa gtcatatatt agacttattt caaatttgtt atatattctt taattaaaat
    23281 cgtgtactcg ttacagaagt agtttctaaa acaaccaagt ttatgtaaaa tttttaaaca
    23341 tagtatttta attatattgg ttaagaatat aagtataatt tatgaaaata tatagagaaa
    23401 aaatataaaa taattctaat aaaaaaagtc tgaatataaa tatattttat atccagactt
    23461 tttacatttt agtttcttgc aactacatat tattcattat cttttatatc aacttttata
    23521 cttaattctt ctaattgctc atcttccgcc aatgtaggag catttgtcat agggcaaaca
    23581 gcattttgat ttttagggaa tgcaataact tctcttatat tatcagttcc tgcaagtaac
    23641 ataacaagtc tatcaagacc aaaagctaga ccagcatgag gaggagtacc atatttaaat
    23701 gcttctaata aataaccaaa cttttcatta gctctttcct ctgtaaaacc aagtgcttta
    23761 aacattcttg attgaacatc agaatttgat attctaacac ttcctccacc tatttcataa
    23821 ccattcaata caatatcata agctttcgct cttaaagatg acttatcacc ttcttctaac
    23881 ttgtctaaat cttcatccat aggtgatgta aatgggtgat gcattgctga gaatgtgcct
    23941 gtttcttcat cttcttcaaa aactgggaac tcagttaccc atagtaagtt atatacattt
    24001 ttatccaata gattaagcct atttgctact tcaagtctaa cttgtcctaa agcatcaaat
    24061 actattgagt ttttatcagc tacaaataaa agtaaatctc ctacttcagc attcattctt
    24121 gtaagtatag cgttcatttc ttcttcattg aagaattttg ctattggaga agttactcct
    24181 tcttgtccta ctttcatcca agcaagacct tttgctctat atgtcttagc atgttcttct
    24241 agtgatgata tttgttttct agtaaagtcg tcagctttgc cttttacgtt tatacctcta
    24301 acactttttc ctggttgtgt agcatcagca aacactttaa atccacagtt acaaacaaca
    24361 tcagatatat ctgttaactc atacccaaat ctagtatctg gtttatctga accatattta
    24421 gacatagctt ctgcataagt catcttagga agtggaagtg ccaattctac atctaaaact
    24481 tctttaaata tcgcttcaag caaaccttca atcatactca taacatcttc ttgctcaaca
    24541 aaagacattt cgcagtctat ttgtgtaaac tctggttgtc tatctgctct taagtcttca
    24601 tctctaaagc atttaactat ttggaaatat ctatccatac cacttaccat caataattgt
    24661 ttatataatt gtggtgattg tggtaatgcg taaaattttc ctggattaac tctacttggt
    24721 actagataat ctcttgctcc ttctggtgtt ggagctatta aaaatggtgt ttctatttcg
    24781 caaaatctat tgtttgataa gtaatttctt gtaatattca ttactttatg tcttaatttt
    24841 aaatttcttt gcatagatgg ttttcttaaa tctaagtatc tatattttaa tcttagtgct
    24901 tcagaaacat catcatcatc ttttatataa ataggcggag tttctgactt gtttaaaagt
    24961 ctaagttcag ttgcaaatat ttctatatca ccagttggca tattagggtt tttagattgt
    25021 ctttcgcata cttttccttt tacagctatt acaaattcag cccctaattt ttcagctttt
    25081 tcaaaagctt ctttatctac atctgtatca aaaactattt gacataatcc acttgtatct
    25141 cttaaatcaa caaatacaag accacctaaa tttctttttt tctgtaccca tcccataagt
    25201 acgacttctt catttatgtt tttttctctc aactctccac agtagtgagt tctctttaaa
    25261 ccctttaaag tttccaagat actacctcca ttttatttaa ttttaatgct atatatttat
    25321 ctaatacaca aaacatcata ctttcttatt ttacagcttt tgctttaatt cttcaactaa
    25381 tgtacttaat tttacagtag tttgctctga tgttttcata ttttttaatg tcgcagtgtc
    25441 atttttaagt tcatcatctc caattactat tgtaaatttt gaatttattt tatctgaata
    25501 tttaaattgt gctttaacac ttctctctat atggtcttta tctgcactta tatgattttg
    25561 tctcaagtct tttaacaatt taaagcttct tgtatttgct tcttcaccta tagttactat
    25621 aaatatatcc gtagattgag ggttttctat ttcaattcca ttaccttcaa gtgtaagtaa
    25681 aagtctttca attcctagtc caaacccaat tccacttata ccttttggtc ctcctaattg
    25741 ttcaacaaga ccatcatatc ttcctccacc acatacagta ctttgagctc ctatatcatt
    25801 agatataatt tcaaaagcag tttttctata gtaatctaag cctctaacta ttgttttatc
    25861 aattacaaag tttatattca tttcctttaa gtattcttga agtttatcaa agtgagattt
    25921 acaatcatca cacaaatggt ctgcaataaa tggtatatct ttgatatttt ctttacatgt
    25981 aggattttta cagtctataa ccctcattgg atttttttct cttctctcat tgcaagtatc
    26041 acacaatata tcagcttttg agtcaagata ctcttttaat aaagcattgt actcttttct
    26101 acaaactgga cagccaactg aatttatact cactaccaaa tcatttaatc caacttcatt
    26161 aaaaaattga acagctagtg cgattatttc agcatccatt gaaggagtat cacttccaag
    26221 tgcttctatt ccaaattggt gaaattgtct ttgtctacca gcttgtggtc tttcatatct
    26281 aaaacaaggt gtcacataaa ataattttgt tggttgagta tctgcatata atttattttc
    26341 tataaatgct cttactacac ctgctgtacc ttctggcttt agggtaattt ctctatcacc
    26401 tttatcttta aaagaataca tttctttttg aactatatct gtagtatctc caacacttct
    26461 cttgaaaagt tctgtatgtt caaatatagg tgttaccatt tcttcatatc catataaagc
    26521 acatatttct ctaaatttat tttctacata acgccatttg taagcctctt ttggagttat
    26581 gtctttagtt cctctaggag ctttagtcaa catttttata tacctcttct ttcttccttt
    26641 ttatcatttc atctactctt tcaatgtatt gttcaacatt ttttacattt tctactcttt
    26701 caattctatc ttcatctaca tagacaaatt tcgatatagc acctgcacct aatgcatagt
    26761 tactttgttt ttcttccata atctgtatat tataaatgca ctcatatcct tctttagcat
    26821 aacctatatt ttctaaattt cccaacatat gtttttgtct gtacatataa tatggattta
    26881 atcccatatc ttttgcatat ttcattgata aatctatcat ttttaccatt tcttcatatt
    26941 gtgttagatg ttctttatac ctatccatat ttatatttaa gttagatgct ctctttatgg
    27001 ctaatgtatg aactgtaagg ctttcaggag aaagtttttt tatctcctcc aatgtgttct
    27061 ttaccatatt taagtcttca tctataagac ctaaaattat atccatattt atattatcaa
    27121 atcccattct acgagccata ttaaagcaat ctactaaatc agatactcta tgctctcttc
    27181 caatctttac caaggtatca tcattcatag tttgaggatt tatactaatt ctactaacat
    27241 tgtgtttttt taacactctt agtttttgtt ctgtaatagt gtcaggtcta cctgcttcta
    27301 cagtaaattc tttaatttta cttaaatcta attcattgaa caatgaattt ataagcaaat
    27361 ccaattcttc ctcatcaagt gtagttggag ttccaccacc aatatataga gtttcaactt
    27421 ctttcttagt atccttaata actttggcaa accccttaat ttcttcaatc aatttataaa
    27481 catattctct ttttaaatgt ccaaattgtt ttaatgaatt tgaaggaaat gaacaatata
    27541 cacatctagt tggacaaaat ggaatactta cataaagaga aattttattt ttatctatag
    27601 gatacataaa taatcgctct cttttagcta tttcaagagc taaatctatt ttctcatcta
    27661 ttataaaata attatctttt agaatggttc ttatgctatt atcatctagc tttttatcca
    27721 ttaaattgtg gacaattttg actggtctaa taccagtcaa aattccccat ggtacataag
    27781 aattaaatac ttgtttaagc actaaaaaca tgcttttttt gatattttct ttacacagct
    27841 ttttaaagtt cctcttactt tcactatcta ggctattaag aaattcttca atcgttttgc
    27901 tgctatcaaa tccatctaaa aattcaagat tcatattttc tatattctgt attgactcgt
    27961 actttaagtt tccattttca tagtattcag tacttgagaa taatacacca ttctcataaa
    28021 tcaatctatt tattaaatgc tttgactcta tattattgtt agcattagag tctttaaact
    28081 taaattcact agtaaacaat tttataagtt ctgctacttc atacttataa tcatgtcctt
    28141 ttaaaaaaac atacaacatc aacttaactc cttctattta acagtagaag aaccccctca
    28201 ctaatatagt tattaaatat tttatacttt tttaaatact aatataaaat tacatgtaag
    28261 gatttgtctt cttttcaacc cctagagtac tggttggtcc atgtccagga tatactctaa
    28321 cagcatcttc atatttacat aatttcttaa gtgatttttc catttggtta aagtctccac
    28381 tatataaatc agttctacca atacttccag caaacaaagt atctcctgtg aacatttcat
    28441 cttcaactct tatacacata catccttgag tatgtcctgg agtatgcaag aaagtcattt
    28501 ttaattcacc aagttcaagc ttgtctttat catttacata gatatctgca tcaaactctt
    28561 gcgctccaca tctcattgta taacttagat ttttattttt gtcatttaat aattcttttt
    28621 catcaacatg tgcaactact ttagcaccag ttttttcttt aatatcctta acatatccta
    28681 tatggtctcc atgcccatga gtaaggataa tatattcaat atttaattca ttttctttta
    28741 caaaacttaa tgtctctact aaacttccac ctgggtctat aactgcacat tttttagtct
    28801 tttcatcacc taatacataa gtatttactc caaaataact atctacaaat ttttttataa
    28861 tcattaaagt tttccccctt aaaaagattt acttgagtct aatagtattg ttactggacc
    28921 atcattagtt aactctacca tcatatgagc tgcaaattga cctgtaccaa ctgtcacacc
    28981 tttatctttt gcttttttta caaattcttc ataaaggtta tttgcaagtt ctggcctagc
    29041 tgcattacta aatccaggtc ttcttccttt tctgcaatct ccatataaag taaattgaga
    29101 tactactaaa agctctccac ctatatccat tagagataaa ttcatcttgt cattttcatc
    29161 ttcaaaaatt ctcagattca atattttatc aatcatataa tctacatcac ttgatttatc
    29221 atcatgagtc actcctaaca atactagtaa ccccttattt atttgaccta tagtgttttc
    29281 atctactgta actttagatg aagatactct ttgcactact gctctcatag tttcctccct
    29341 attattttaa ctctaatttg tcattctata aatactttct acaccaggaa ttgcttttaa
    29401 ttttttcatt agaattttta attcctctgt gtcattaacc tctacaagta agtttatact
    29461 tacaatacta tccttacctt ttcttgcatt tattccgttt aaactaagtt tatccattgc
    29521 aacaatatga gttatttcat taacaattcc tcttctatca atagctttta tttgaatttc
    29581 cgcttcaaac ttaccttttc ctgctgaact ccatgaaacc tccaccaatc tattttggaa
    29641 gaaatcacca ttattgtttg catttgggca atcagctctg tgtatggcta ctcccctacc
    29701 cttagtaata tatcctacaa tttcatctcc tggaagagga ttacaacact tagcaaatct
    29761 tactaagata ttgtcaagcc cttttactat aattccttgt gatgcgtttt tctttctctt
    29821 tttactgtat tctgtttcac ttatattgtg tttatttaaa tcttcttctt gtatctgttg
    29881 ttccttttta gcttttttaa cctcttttaa atataggtct tttaccttag ataccacttg
    29941 agatgacatt ataccaccat aacctacagt ggcaattaag tcctcaacac ttggctgatt
    30001 aaatttctta gcaactaatg ccatatattt ttcaacaaga gggtcttttt gtggtatatc
    30061 atgcttctta aattctcttt ctaatattgt atttccacgg tcaacatttt cttctcttct
    30121 ttccttcttg aaaaattgtc taattctact ttttgcattt ggggtcttca ctatattaag
    30181 ccaatcccta cttggtccat tagaattaga tgatgtaagt acctcgacaa tattaccatt
    30241 ttgaagctta taatcaatag gaactattct tccatcaatt ttagctccta cacatttatt
    30301 gcctacattt gtatgaaccc tataggcaaa atctataggt gttgattctg ctggtaattc
    30361 aattacgtct ccttttggcg taaatacata aacttgacta ttaaatacat cttctttaag
    30421 tgcatccata aactcttgtg ggtcttttaa atctttttcc cactccatca tctgtctcaa
    30481 ccattgaagt ttttcttcaa cctttgattc tttgctagag ataccttctt tatatttcca
    30541 atgggctgct ataccatttt cagctatatt atgcatctca tgagttctta tttgtatctc
    30601 taaaggttct ccatcaggac ctacaacagt ggtgtgtaat gattgataca tattgggttt
    30661 tggcattgct atataatctt taaatcttcc tggaataggt ctccataatg tatgaactat
    30721 accaagtact gcatagcaat cctttacatt atcaaccaat atccttactg ctgttaaatc
    30781 aaaaatctct tcaaaagtct tatgtttttt ttgcatcttt ctatatatac tgtaaaaatg
    30841 ctttggtcta ccatatacat cacatggtat acttaaatct ttaaacttat ctttaagaag
    30901 ttcaacaatt ccttgaatat aatcctctct ttgacttctc ttcattgata ctctactaac
    30961 taaatcataa tacccttctg ggtccataaa tcttaatgct ctgtcttcta gttcccactt
    31021 tatcttggaa attcctaatc tatgagctat accaccataa atttcaagtg tctcttttgc
    31081 tttatacttt gctttttctg gaggcatgta ttttaatgtt ctcatgttgt gcaatctatc
    31141 agcaagtttt ataagtataa cccttatatc cttcgccatt gccaaaaaca tttttcttaa
    31201 attttcagat tgagtttctt ctttcgaacg atattttatt tgaccaagtt tagttactcc
    31261 atcaaccaaa tccgctactt ctttgccaaa ttcttttttt atgtcttcat aggtatattc
    31321 tgtatcttct acaacatcat gaagtaaacc tgctgttata gtttcaatat ctaattccat
    31381 ctctattaaa atatttgcaa ctgctaatgg atgaataaag tatggttccc cagattttct
    31441 atattgtcct tcatgcgctt tttttgcaag attataggct ttttcaacca tttcaaaatt
    31501 tgcattaggt gcataaactt ttattttttc aattatttct tgtaattctt tatcatgcat
    31561 ataaccacct attttgtata aaattttaat atatatgaaa atagttggtg ttataccaac
    31621 tattataaaa gtactagtat tttataagag aattaacatc atagttagac aacttttctc
    31681 tcccatttaa gaattttagc tcaatcaaaa attgcataga aactacttct ccacctaatt
    31741 tttctacaag cttggcagcg gcttccatag ttccaccagt agccaataaa tcatctacta
    31801 ttgctacttt ttgacccttt ttaattgcat ccttatgtat ctctaaagta tctgtaccat
    31861 actctaatcc atactcataa ctctcaactt cacctggtaa ctttccaggt tttctaattg
    31921 gaacaaatcc tactcctaaa gcatatgcta caggtgtacc catcaaaaat cctcttgctt
    31981 ctggtcctat tactaaatct acatctttat ctttcaattc ttcaactata gaatccactg
    32041 catatttaaa tgcatctcca tctttaaata gagttgtcac atctttaaaa tctatgcctg
    32101 gctttgggaa atcatcaata ttccttataa agttttttaa atccatggga aacgcctcct
    32161 aataactgct attgtattgt ttttctaaat tttttagatt attgagtatt aaactctcat
    32221 ttaagtctaa ttttttctgt ggttttggca atattcttat gagtacagaa tttttttcat
    32281 aatttaaatt aaagtcaagt aaatttaatt ctctaaatac atttaatatc gtaaaaaact
    32341 ttaatggaat tacattaaaa gtttccctta tatttaccat atccaaatca atttcttttt
    32401 ttatgagtgc ttgtttataa attgttataa attcatctct tgatggaact atattactaa
    32461 ctacattctt taaatataat aggtctgttt tattataata ttttataatt tcactttctt
    32521 ctcttttatt ctcatatata tacgaatatt cgcctttatt gtataaataa tcataaagaa
    32581 taatattatt atatcttttt aagtctattt tatcaatatt aggagaaaat ataagctcta
    32641 atttatcagt atttttgcta acatcttcaa taatattgta atttatgtta aaatccaaat
    32701 ctataagtga tatatctgac attgctctat aaaatccatt tatagaatta gatattatta
    32761 gtgtatcttt ttcaaaataa tcaaatatat ttatatctgt atttccatca aataattcaa
    32821 aatcagacac agatatatta tacaggcttt gagtatcttt tggtattatt tttgacatta
    32881 atttcaatga aagtttgtca ttacttgcac ttttaggtcg agccaatcgt atatctttaa
    32941 gtaaaaattg tactttccta tttcccatat agttattttc atctaattgg aatagtacat
    33001 ctaccttgtc tcctacttta tacattgatt ttaaatgact catattaaat cctatacatt
    33061 catagctttt ctctttttct atgcttaatt tcagatgttg ttgattctta cctataactt
    33121 tcaaatcttt taaaatataa tttctaacta taaatctagg attaggatta tttaatccaa
    33181 agggttctaa cttatgtaat tcttctacaa gatttaaatt tattacattt tcttgtaatt
    33241 caaactcaac atttacattc tctatcatat catcttcagt taaattataa tctgcaaatt
    33301 tatttatttc atttgctaaa atttctacat tgtctgaatc caaggataac cctgctgctt
    33361 gttcatgacc accaaactta gtcatcaagt ctttgcactt tatgagagct tcaaatatat
    33421 taaatccttt tattgacctc gctgaaccag tagcctcccc attttctata cctagaagaa
    33481 ttgttggttt gtagtatttt tcagttaact ttgatgctac aattcctatt attccatgtt
    33541 gccaaccctc tttagctaaa accaacacct tgtcatcatt gtatctgcta ttagacttta
    33601 gcatttcctc agcttcatga tacatcttag cttctatcat ctgtctttca ttattttttt
    33661 cttccaatat actagctatt tctactgcct cttcttgtga ttgagtagta aatagctcta
    33721 ctcctaagta agaatatccc aatcgacctg aagcatttat cctaggacct attgcaaaac
    33781 ctatatgaga agaacctatc ttatcacttt ctacaccaca aacttttata agctccctta
    33841 atccaatatt tttgccttct ttcattgatt ttaaaccatt ttttacaatt attctatttt
    33901 catctataag tggaacaata tcacaaatag ttgctagtgt tactatttcc aaataattat
    33961 acatactagt tttaaattct tcttttggag tcaaggcttg aatcatttta aatgctacac
    34021 cacatccaca tagtgaatca aatggataat tacaatcttc ttgttttgga tttattactg
    34081 caaaggctga tggaatttca ctttgacatt catgatggtc tgtaattata acatctatgc
    34141 ccaaatcatt cgctgtattg acctcagaca ctgatgtaat tccacagtca acacttataa
    34201 tcaagtcaca tcccctagag cttataagct taatagcatc ttcatttata ccataccctt
    34261 cttctaatct attaggaata tagtaatcaa cattgtaacc tatactctta aaatataagc
    34321 acaagataga tgtagatgat acaccatcaa catcataatc tccgtaaatc caaatttttt
    34381 cgttcttctc aactgctaac tttattctat caacagcttt ttgcatatct ttcattaaaa
    34441 aagggtctct taaataatct aaagatggat tcataaatat ctctgaatct ttttcagtac
    34501 atatccctct attttttaaa atttggcaga tttccgggga aatattaagt tttttgctaa
    34561 attcactttc ctctatctta cctttatact tcaatatcca ttttttcaaa tttaattccc
    34621 cctaatcttt tttgtatttt gacctaatac ttcttataac taagcaaagg cctattataa
    34681 aggatataaa gaaacccgct attgcagtaa attttatcca tatattattt tctatatttg
    34741 gagatgcaat tataagagaa gaacctacta taatagatgc caacactaaa ctcaaagata
    34801 tttgagatgt tagctctatt atacaatttt ctaacctcgt cattttaaca tcttcaattt
    34861 gcatttttat attatttctt tctatatttt ttaaaattgc ttttaattgc tttggaatag
    34921 tttttatgtc cagtaatatt tcttctacat tttgtcttga acttaaaaca acatttttag
    34981 ggttaaattt actcttataa tgatgtttca taaactcttt tcccatagaa ccaaaagaaa
    35041 aatctgtgtt taaagttcta cttgttcctt ctaatgttat aactgtcttt gcaagagtta
    35101 caaattgtgc aggcatgact attttatact gtctaaaaaa cctaaatagt tcatttaata
    35161 tatctgttac atttattttt tctatagaaa tatcatagta ataatgtatt agataaagta
    35221 aatcctgtct aagtccctta atatcagcct ctccatttaa tgcatccatt tctatcaata
    35281 gatatattat cttgtcaaca ttcttttcta cacctgctaa tgcaatttca tttaattggt
    35341 ttaaagtttt attatcaata attccaatca ttccaaaatc tatatatgct atcttatttt
    35401 tagcaactac gaatatatta cctggatgtg ggtctgcatg aaaaaatcca tgagataata
    35461 cctgtgtaaa aaatgaccta acgcctattt caacaatagt ttttgtgtta taacctagtc
    35521 tccttatttt ttctacatca cttaactttg taccattaac tttctccatt actaaaattt
    35581 tcttagtatt atattctgaa tatactttag gtatatatac ttcatcacta tttttaaata
    35641 tcctagagaa tttagttgca tttatagctt caaatgtata atcaagttcc ctcattaatt
    35701 gagtttgaaa ttcttcaatc atctgtacta aatctaaatt aaaatccttt tttaaatctt
    35761 ttaaagtatt tgcaatagtt ctaagaatct ctaaatcaga ttttattatt ttctctatat
    35821 ttggtctttg aatttttacg attacttctt ctcccgtttt aagtacacct tcataaactt
    35881 gaccaattga agctgctgct attgcttctt ctttaaattc taagaatatt tcttctatac
    35941 ttaaacccac ttcttcttta aatatatcaa tagctatatt tgaatcaaat ttctctacat
    36001 catctctgag ttttgatatt tcatcaatta tatcctggtc taacaagtct tttcttgtac
    36061 tcaatatctg tcctattttt atatatgtag gtcctagttc ttctaaaact cttttcattc
    36121 tctcaccagt tgtcatattt tttatttctt ctggtgggtc aaatagtggt attttatagg
    36181 caattccttc tatatttagt ctttctacaa taaaactaaa accatacttt attaaaacat
    36241 atccaatttc tctatatcgc tttaaatttc tataacttat tctcaaatct tttcactcct
    36301 aagataggta tacccaaaaa cttacacctt aatttatcgt atatgacatt atttcaccaa
    36361 ttgatattct aatatcctat atcctcttta atatttaatt ttatcacata tactaccaat
    36421 tttattaaaa ttatttaact cagttaattt ttatttttga cattttatat ctggtttaat
    36481 caaatacttt ttgaaaacta aaatatcaac aaaatctaat aaaataaatt agtggttgcc
    36541 tcaaaacaga tttaaatatg ttttgaaaca accactcttt aaactaatat ttttgatttt
    36601 gtgctgcctc taaataccat tcataagatt catcaatata ttttttatct tcttctgtaa
    36661 gctctctaac aaccttacca ggagaaccca ttatcaaaac acctggcgga aacttcttat
    36721 tagatgtaat tagtgtacct gctcctatca gagtatattc tcctatttca gcattatcca
    36781 atattattga acccatacca attaacacat tatcactaat tttacaacca tgaactatag
    36841 acctatgccc aactgttaca ttgttaccaa ttatagtttc tgtatctcca tgcactatag
    36901 aacaatcttg tatattagtg ttttctccaa tagttattgg tccttcatca cccctaacta
    36961 ctgcattgta ccatatactt gaatctttgc ctattttaac atttccaatt acatctgctg
    37021 attttgccac aaatacactt tcgtcaatta aaggtttatc ttctaaataa tctctaatca
    37081 tatataaaca actcctttaa aatataaatt tagttggttg ctccttctaa cattaactta
    37141 gcttgtatca ttatagagca ctctgtacgt tttctttgca tttcacatcc taattcatat
    37201 ggttttgata tgtcattatt aaagtttatc gcatttgcat gacatccacc tgaacagtaa
    37261 aacttattcc aacattgttt acactcttct ttagtatata catgagcttc tctaaacata
    37321 ttttttaaat tttctggaag tactatttct tcatcaaata tatttgccat tttaaattct
    37381 tcatttccaa caaattgatg acatggatag atatctccat ttggagttac agaaagatac
    37441 tcatttccag ctccacatcc agttattctc tttattacac atggaccttg atttaggtct
    37501 atcataaaat ggaaaaattt aaatccatct ccttgtaatt gtctatctgc atactctaca
    37561 gcaaattttt cgtactcttc aaatatctta ggtaaatctt cttctctaag tgcatatgga
    37621 ttactctcat ctccaacgac tggctctaca gaggttaatt taaatcctaa atctgcaaaa
    37681 tgcataacat cttttgaaaa atctaaatta tctcttgtaa aagtacctct tatataataa
    37741 tacttatctt ttgctctttt ttccacaagt ttcttaaatc gtggtaatgt aatatcataa
    37801 cttcctttat catttaaagt tggtctcata ttatcattaa cttcttttct tccatctaaa
    37861 cttaaaacta cattatgcat attttcattt atataatcta taatctcatc atttagcagt
    37921 acaccatttg tagttattgt aaatcttata tttttattat aatctttttc tacacttctt
    37981 ccatagtcaa ccagttgttt tacaacttca aagttcatca aaggctctcc accaaagaaa
    38041 tctatctcta ggttctttct acttcctgaa ttagcaatca aatagtcaat tgctgcctta
    38101 ccaacttcaa aactcataag ttctttttct cctccaaaat caccttgagc tgcaaaacag
    38161 tatttacatt ttaagttaca atcatgagct acatttaaac ataaagcttt tactacaggc
    38221 tctctatgta caaagcttgg atgatattga tatgtatctt ctgtatataa aagaccctct
    38281 ttttccaaat ttaatatttc ttcatatgct tcatttattt tttcttcttg atattttgat
    38341 tttaatatct ctaatatttc ttctttactt ttttctttat aaaaatctaa caaatcgtat
    38401 gcaacatcat ctaacacatg aacagctcca ccattcacat ctaaaacgat attatatcca
    38461 ttcatagaaa atttatgtat catactcata ccctacctcc taaattacta tctatactaa
    38521 agttttttat tattttaatt aaactttacc cttcattata tcataagttt acataaaata
    38581 catacaatac aaaaatgact tactctccgt aagtcatttc attaaataca ctatatcccc
    38641 ctaaattggc gcactaatat attaccaaat aggtattagg ctcctataaa ataaattttg
    38701 tatctgattt tatattgttt taatcttatt aaaatataat aatttctttt ttcctgataa
    38761 ttttgtataa ataagtgctt gtatttaatt tgtcttcttt agattgctga tttctttggg
    38821 tactttaggc tgatgaataa tccaaggaca ggtactatta gctgacaaaa tagcagtgct
    38881 aagtgccaat gagctagcaa acttcataaa tcttacaata aactttttca taacttaact
    38941 ctccctcata catattttac ttatacttta gcttttaact tacctaatac taacatcaca
    39001 aaaatccaaa acatggcaaa tgcgctatat gtgaaataat ctacaaatat actcaaagaa
    39061 agacttgaaa ttgttatcaa taacactact gtagatataa atatagcagt tttcttataa
    39121 accaactttt ccctatcact tatagggtta ctcctatgtt ctattgggca cagaacacaa
    39181 attccaaccc aactaacact tgcaataatc ataataatat ttttaaataa gtctatattt
    39241 atattttcta taattaagat tgtggataaa attataaaaa taaaagttaa taagcatcta
    39301 aaataattgt ctgcatgata ccctcctgaa aattgtctta ttggacaata acacattaaa
    39361 aaaaataata catatgtaaa tttaccaaat aaaaaaccta taaacagcat ggtggttata
    39421 ttaactatga aagctattaa tatttcaaat ccataagaat agactttaga ttcattatta
    39481 tctatcatat tattacaaat taaaacagat gtcatttttt cagcatatct tttaaacatt
    39541 tttacctcca aaaaaatttt acatatatag agtacattct tttttgagaa aattcaatat
    39601 actttacaaa actagttgtt aaaaatgtaa aactggtcaa aaaaagacct atataatttt
    39661 cttcttatta tttatataac aaaacaatta taataaaaca attttatcaa gctttatatg
    39721 cataaaaagc ctatgtcaaa atagaaacaa accttttccc ctttgttaaa aggctaaaaa
    39781 tatatttcat ttcatataaa aagagtacct cacaaactaa aaagttcctt ttgagatacc
    39841 cattttattt attacaaaat atcaaatttt atttatttgc atatattccc catatatttt
    39901 agccttttaa ctttgttttt gttttacata ctgtagtgct tttctattct gttttactgc
    39961 ttcaagacaa actttttctg tttgattatc aacaaattct aaagctctat aattttgctt
    40021 tactgcttct aaacatattt tttctgtttg attatttaca tatttcaaca ttctataatc
    40081 ttgttttact gcttttagac aaacttcttc tgtttgttct ttcacatatt taagcgcttc
    40141 ataactttga ttaactgctt ctaaacaaat cttttctgtt tgttccttta cataagctaa
    40201 agcattatag ttttctctta ctgcctctaa acatattaat tctgtctgat tttctacata
    40261 tttaagaatt tttccatttt gctttactgc ttctacaaat attctttctg ttggtatctt
    40321 tacataactt aatatttctt ttatttcaag ttcttcaaat gaacctctta ctgcttctat
    40381 acatatattt tcagtctgtt tttttacata ttgcagtact tttccatttt gtctaactga
    40441 ctccaagcaa atttcttctg tttgctcttt tacgtactgt aatgctcttc catcaattct
    40501 tactgcttcc aaacaaatct cttctgtttg cttatttaca tactgtagag ccatataact
    40561 tctttttact gcttccatgc atattttttc tgtctgttca tttacataat acaatgcaaa
    40621 gtcattctgt tttactgctt ctatacatat ttcttcagtt tgctctttta cacaagatag
    40681 tgcactgtag ttttctttta cagcttctaa acatatacta ttactttgtt cctttacata
    40741 agctaaagat tttccatctt tttttacagc ttctaaacat atggctggag tttgctcttt
    40801 tattcctttt aatatctctg caacatcatt tttagatgcc ctttctataa atgctagaca
    40861 tacatcttct gtttgctctt ccacaaattt aaatacttcc atgacatcat aagttgatgc
    40921 attttttata gcctcaatta gtacattttc tgtcttatta tttatgtagt acaaagctct
    40981 gtagttctgt tttactgctt ctatacaaat atcctcattt tgatttgtta tatacttaag
    41041 agatttatat gtctgtttta ctgcttccat acatacttta taagtcttgt cctttacaaa
    41101 ctcaattata gtaccatcct gctttactgc tagcagacac atctcttctg tttggttttc
    41161 tatgtctttt aactctctgt aatcatattt tactctgctt aaatcattta aatgtacttt
    41221 gttatcttcc tttaaatctt cttcattatt ttctatatca tactcaaatt cttcatcatc
    41281 taaatagtca tttttttctg gtaaaaatat agatttaatt ttattcttta tatccataaa
    41341 attatcctcc tttaatatga ttctatcatt aaagtaaatc gtttttaagc acttatttaa
    41401 ttaattttat attaagttta tttaaaagtc tattcattgt cgatagatta tattttatat
    41461 taataatttt atcttatata tgtggggtaa taaagttaat agtggacatt ttccatccca
    41521 ttagtataca ctatttatat ttattactca tttatagtga tttttttcaa attttttctt
    41581 atttactata tcatatttat tcaatttatg atattattta aatataatat aagttttata
    41641 aagtatatta aatatattat aagatggagg tcgagtaatt ttgaaaggaa acatagtaca
    41701 atataatttt gcagatatag aggaagaagt atattcgttg gattatgcta tagcatggaa
    41761 tacagacgag gaaaacgtaa acataatacc atttacaaat aaattctgta aagaatctat
    41821 agaatctttt tgcctaggta aaattaataa ctttgttgaa atattaaatg aaggttttgt
    41881 tgaaaatcat cattatgtac atttagataa aatgattagt gttcctaaga aaaaggttaa
    41941 tttagtatat caacaagaca cacatggtta tcttctaaga gatgacaatg acaaccttat
    42001 tcctgcaaaa ataacttcag agcaatctaa atctatatca agtaagatgg agttattctg
    42061 tgctggtgaa gaaaaatgtt taattaatat attactaaag gctgacccat catatatttt
    42121 agatgtagat agtataaaag acaaaaacat tctaaatcta ggttatgagt ctattgacag
    42181 atataaagaa tacaattttg atgatgataa gattttaata ttttttataa ataaaaaacg
    42241 ttattcagtg attatgaaaa aaacaaacaa ctctgataac gatttagttt ccagaaacaa
    42301 tgctattaaa gaattattta ctaataaagc aggaaatctt aactaataat atagttttaa
    42361 attatgttat tgattaaatt aataatagta tacaaagagg ctgtctcaaa atagagataa
    42421 gcttcttttt cattgttgaa ataataaaat atatttaatt ctatataaaa gagagtgcct
    42481 cataaactaa aaagttcatt ttgagacacc ctcctattta taataaacaa ataaatcctt
    42541 aaacctattg agtgattata atttttagat tgggttatct tgcattttta aatttaaggt
    42601 aactttctac tctaaacttt aatctctaat caatatcatc aactgtaaca acttacaatt
    42661 aaatctttaa taatcaaact ttagactatc tatattaaaa ctcaaattag atttaggatt
    42721 tatttggata tcacatttat tttattaact agtaatcaat tatttttact tataaactta
    42781 ttcttcccta attctattat taaatacttt taattatttt tcaataccat atttttttaa
    42841 tttttactat taaatcacaa aatattatct tactccatca caactacttt tgatatcatt
    42901 tgagaatgaa tcttcatttt aatagtactt ttattgataa taacatccat atatactata
    42961 gccacaatgc agttttgctt tttcacaaaa tgactattac aaagattaag taattcttag
    43021 taatctttta taaaaaagag atgcttcaaa tttttatttt gaaacatctc ctttttaatt
    43081 cgtaaaatat aattttgtta tttagaaaaa tgaattttag taactatata acattaatta
    43141 tatattaaaa gtcttttcta tatagctttt tatttcttca gcagtaccca tattaagtac
    43201 atcatcagct aattttttca tatctgactc tttaactgaa gttattaact ttctagcagg
    43261 aagtattgat attggagaca tagagaactc gtctaatccc atacctaaaa gtattggtat
    43321 cattttttgg tctccagcag attctccaca catacctgcc catttgcctt ctttatgtgc
    43381 attgtctata acagtcttta taagtcttaa aacagctgga ttaaattgat tgtaaagata
    43441 gcttatcttt tgattcattc tatcaactgc acaagtgtat tgaattaaat cattagttcc
    43501 tatagagaag aaatctacgt gtttagccaa tacatcagat atcacagcag cagatggaac
    43561 ttctatcatc ataccaattt ctacatcttt agcataagcc acaccttcag aatcaagttc
    43621 tgctaaaact tcttttacaa cttctttagc ttgtaacaac tcttctaaag atgaaatcat
    43681 tgggaacatg attcttaatc ttccatgaac actagctcta tataaagctc tcaattgagt
    43741 cttaaatata tcttttctat ctaggcaaag tcttattgct ctgtaaccta agaatggatt
    43801 catttcaggt tccatactta aataacttaa ttctttatct ccacctatat ccaatgttct
    43861 tataactata ggctttccat ccattccttc taatacagct ttatatgctt catattgtat
    43921 ttcttctgtt gggaaagaat ctctatccat gtataaaaat tctgttctat aaagaccaac
    43981 accttcagca tcattcttta ttagaccttc tatatcgtta ggtgttccta tgttaccagc
    44041 taattccaca tgttttccgt ctaaagttac agatggtttt ccttttaata gctgtaatgc
    44101 ttttctctca tcttcatact tagcttttaa ttcagtatat ttatttatag tctcttcatc
    44161 aggattcacg attacttcac ctgtgtctcc attgaaaacc acaaaatctc catccttaac
    44221 ttttgaagtt atatcattta atccaacgat tgcagctatt tctaaagttc tagacatgat
    44281 agcagtatga gaagttcttc cacctatatc agttaaaaat cctaaaacca tttttttatt
    44341 catagttgct gtgtctgatg gagttaaatc atgtgctatt aatacaactt cttcgcttaa
    44401 agcagataaa tcaacaactt taattcctaa tatatgtcta agtattctat ttgtaacatc
    44461 ttttatatct gccgctcttt ctttcatgta ctcattgtcc atagactcaa acatgctaac
    44521 aaacatctct tttatttcat ttaaagcata ctctgcattt acactttcag ttttaatttt
    44581 atctattgca gaaccaatta actctgggtc ttctaaaact aataaatgtg cttcaaatat
    44641 ttctgcttcc tctgctccta attcttcaga agccttttcc ttaactttta ctaattcttc
    44701 tttagaaact gcaactgcat cttctaattt agcaatttca gcttcaacat tatctataga
    44761 ttttttttca attactaatt cactatgttc aactactaac gctttcccta aagcaacacc
    44821 aggagaagca cctattcctt tgtaagccat aaccctacct cctaaaaaat atcatctagc
    44881 acataaactt tataaattta tataaataat ttacattttt atagttttat ttacttataa
    44941 ttttttataa aatttatcat tttgtatata attaaaaaca cactttttat aattttgcat
    45001 aaaaaaagag tgtttcatat atttctaaac actcttttct actttttata attatctaat
    45061 tgtaaaagaa gcttcagctg gactagagaa taatataata cctcctgtac acacaccttt
    45121 tcctttaaat ttagcaatat atgaaccctt ttttagccct ttaaaagtta ctacaccagt
    45181 tttagtagtt ccagctaatc taaagcctac atactgagat gatacaattt tgccagcctt
    45241 atatacatct atatttcctt caaatcctaa aatatttcca agcgtagatg ttactctcca
    45301 agataactgc ccaccactca ttgcagttaa attagcagta ccacctggaa caacaacaga
    45361 tttagtcgaa ggcgaatctg agtttggttg gccaattaat tcaactgttt gtgaatttcc
    45421 agtaccttca taatcatttc catcagcaaa agctgtaaat ggaatcatta ctgcgcaaag
    45481 cgttagtaat aaagcaattg tttttttcat ttgttttccc cctatttttt tatattattc
    45541 tcaattacag tttcctttct ttacgtgcct taataatcat attgtaatta tgctcagcag
    45601 tttcaaatgc tcctaccaaa ggagttataa ttacactttc tattaagcac aattcatcat
    45661 agtttaaact agcttctcca gcatagaaat tatttaactt atctaaatct attctagtta
    45721 aaacggaaac tgattccatt gagaatttgt aaacatcaat aagtttatat aatccaccta
    45781 tatagttttt tgctacttca tggctcttaa aaaattcatc ttttcctgta tgtaggttta
    45841 cttccataat tccctccatt gttcttatat ttttaacctt caaataatat ttacttcttc
    45901 ttcaaaacag aatattctat cacggtagaa tattctgttt ttttcattct atcatgatag
    45961 aataatattt tcaatcattt tttctattct attttattta aatagaatag aaaaaaagaa
    46021 cacttaatat ttttcaagct aatatattaa gtgttctttt tatattttta caagattata
    46081 taaaattatt ctttaatcaa ttaaagtgtt tttctatttc ttaataataa gaaatgatat
    46141 aaaattgtat cattttaaag attttgtgat ataataaaag caagaagaac tacaatctat
    46201 ttaggcgtag agtggagttc ataataaacg tagtttattt tttgaattta aatataaatt
    46261 taaattcaac ctgtaagtca ctctttgcac gagagtggct ttttgctttt ttgaatagtt
    46321 cactgattaa gtaaactacc acactagctg ttaaactagc taaaacatta aacaaaaagt
    46381 tatccatgta actcacctcc cctcgaatcg ttgggaggat aatcttttgt acatgaactc
    46441 cactctatag attgtatatt acgctcttct tgctaaattt aattatacca tattttgtaa
    46501 tatcagttaa gagtttgcac tacaataatc agaaagcttg ttccactcac aatcaacccc
    46561 tattatttta agaatatcat tgacttggct atttaaatct ttaatatctg atttagacat
    46621 taaaggatct ttcattattt catttagaaa aatactatca atgattaaac atacattttc
    46681 tgataaatat tcacgtttta ctgagattac tgttccaact gtactttctt ttagtgtctt
    46741 atatgctaca ctcaatatgc atggttctaa attaattcct tctagtactg tttcaaagta
    46801 atattttatt cttatatttt catttccaat aaatatttct tcattgctgc ttatcttctt
    46861 taattttctc attatgccta acatcttagc attcacttca tagtttattc cacatctctt
    46921 tgatgctaaa ctcgacatca agtctctttc tgaataaatt acccattgac cattatagga
    46981 aaatgatgtt ttagcgatat ccatttctcc ttttccagta atattaaatt taggctctag
    47041 tttatttatt aagtaaatct cataaaaatc catagtatat ttattatcta attctatata
    47101 ctcaaatctt aaattcttat tacaccaatt ttctttttta ttacttaaat gactagcgtg
    47161 tctatcgcag atattctctg ttttcccaac gtataaacat tcttccgtat ctagattaat
    47221 atatttataa acataataca ttttttattt tctccaatta ttatttatag taatatcctt
    47281 cttcatttac tagaccttga tcaaataaat ttctcctaac agtttcaagt ctgacaggat
    47341 ctttgctata atcaacttct ctttcttctt ttttaacatc ttcatcatcc caatacttat
    47401 ccaattcttc tttaaacttt aaatcatctt tttgatgaat tataacttca cttattttat
    47461 ttgaaagtgt tgatttaaaa aaatcataat tctttgtatt tataatatca gtattatctc
    47521 tttcaaatgc tatagcaata gacttattaa aagcattcag ataaacatct ttcttaaaat
    47581 caaactcttt aaaatcttct ttaaatagtt taatcgtccc tgttgtaaaa atattttcat
    47641 ttggtatata aaaactaaaa gacacttcat tgtcctgctt ctttttaggt atataatctt
    47701 caaccaatat atctttatca aaatattttc taggttcata atctatcaca gaaaaaatta
    47761 tttcatctat ttttctattt tttctttttt cttcttttat tgaaacaacc atgttgcctt
    47821 ttttatttat ttcattcatt gctcttttta gaacattttg tttaaaatat ttatactctg
    47881 gatatacatt atcttttaat ttcaaataaa atcttaattc atctaactta tattttatct
    47941 ccacttcctt attttctctg ctccatagtc taaataaagt gtataacctc tgagtatatg
    48001 cacctctaaa attaaataac actgataaat taatagctgt atatccattt ttctttttct
    48061 gcatttttac aaaatcagtt atatgattgt atagaacttc atgcatcata attgtataaa
    48121 tttggtctgt atgatccaat tcatatgtat taattaaacc acttccaaaa gtttttaact
    48181 tgccagttgt ctcttctata tagtcaaatt ccaaaacact ttgttgaaac atgttcaaaa
    48241 tttcttttat atttttatgc tcataatcat tattgttttt cataaatact tttagttctt
    48301 cttttgatat agttgtaaca taagatgaat tttgctgttt ctgagcatta aaaagtattt
    48361 tgtaaaataa tttattttcc acatttgtaa aatcatattt actttttatt aaattatttg
    48421 gttgcattaa aatttctttt ttttccaaaa tctatcacct catataaatt ataataccta
    48481 atttataaaa aggtcaacca tttttgaaag acgctcacct ttgaccctta aaatgatgac
    48541 ctttgaccct taaaatgatg acctttgacc cttaaaatga tgacctttcg acccttaaaa
    48601 tgatgacctt tgacccttaa aatgatgacc tttgaccctt aaaatgatga cctttcatct
    48661 ctgaaaccta gatataccaa ttattatagc ttgtctaaaa actattaaag actttaaaaa
    48721 ctgttttaaa aactattaaa gactagaaaa gatgttctga actattcttt ttttatatta
    48781 aaaaacttta gtatttattt ttctagtata ctttaaacac ttactaaaaa taatttctta
    48841 taaaagattg gagatgaaga attttttatg cttgaaaaca gaaaacaaat tcagaaagaa
    48901 atttacttac caatagcaac atattgttta aaagataagc aatattatat aaaagaatat
    48961 ggagcactat taataaagag tgctgaaaat agtgacattt ttaataccca taaaaataga
    49021 attttgtata agaataaact tgatattcaa gaaatagcaa atgaactgaa tgtaaataaa
    49081 gcaacaatcc aaagaaatat aaaaaaacta gagaagttag attttaaaat tctaaaaata
    49141 gaaaataccc taaatggaat tgtatattgt ttaccatcag aaaataatat tgatttaaac
    49201 aaatttgcat tgataaatta tgaaatgctt aaaaaaatgg tcaataaatt taaatcaaat
    49261 acaataaaag tatatttttt acttaaaaca atcactacag aaactaattt taaacctgca
    49321 actaatagtt ttatagcaga aaatatagga ctaagttcta aaagtaaaaa taatttagat
    49381 atcataactt cttctgttaa aactttagaa gaaaataaat acattgaaac aaaaaaagta
    49441 aatgtatatg aatatgacaa agaaaaactt agagaagttc caaaaataag aaaagtttat
    49501 cggatttgta attttgatga gtggaaaaag gaaattgata aaaataaagt ttattaaata
    49561 tagcttaaaa gcatttaaag gcttgtctga cgtgttttaa ttacaaactt gatgttattt
    49621 acctaaaaag atacaaattt gcttaaaaat ggaaatagaa ggtcaaatat ataaatagag
    49681 aaccaaataa aattatattg gctctctatt tatcttcttc ccaatcctca ataaactttt
    49741 ctaaactttt taatttattt tcagacactt cttcatcatc atgcagagca cttataaaac
    49801 tttcaaaaga atttttgtga aagaaactaa agaaactttt agtctcaact tttaaatatt
    49861 cttctttttc tattaatgct ttataatatg taaatctatc tttcttaaca acctctaaaa
    49921 atcctctacc aactaatctc ctcaagaaag ttaacatagt tgattttttc cattcgtact
    49981 tttcgcctag tgtcttcaaa atttcagtag atgcaacttc tttatcttcg ctgtcccaaa
    50041 taaacatcat aaccaacaat tcactttctg gaatcttttt tatcttcata aagtacacca
    50101 ccttctaaaa gtatatctaa aatactataa caatagagtt aactacacaa tactaaatgt
    50161 ctaaaaatgt tttaccaatt tttaccaatt aatttttctt taaaaaaaaa ttaagcagga
    50221 gtaaaaaaaa taaaatatta tcaaaattta tcaatataaa aagaaatata agtataaatt
    50281 tttctctttg tatattgatt gtatacaatt ctatttttgt aaaattaaat tttaaaaaat
    50341 aatcaaaaat ggtaaatttt gacatttgct taatgtcaaa ttaggtgtta caattgtagc
    50401 ataaagaaga tatatatcga aataatacat ataaaactac gtacgtaaaa tatttttaaa
    50461 aataatcaaa aatcttaatt ttcaatattg aatacaatta aattttcaaa ctcatctagg
    50521 gcatgacata caaaagcaaa aaacattaat ttagtatctg ataaaaattt aaaaattgta
    50581 aaagggggaa gtctgttatt tttcagaagc aatgtaattg taaaaatata aatcagaata
    50641 ttttgaaaat atgggggata aacttatgat ggatttagaa aaggaaaaac atgaaataat
    50701 tgaaattata aaaaaattaa atattgataa agataaaagg aatttattga tgataaaaaa
    50761 ttatatcctt ggacttttaa aaaaatagaa gaatagcaat tgctattctt ctatttctag
    50821 taaggaatct atcattttat caaatacagt cagatatttt ttattcaatt tgaacatttt
    50881 agttataatt tctctttcta cttcattttc cattttactt agttcaccaa caagatgggc
    50941 aaattgctca tctaattctt tgataaaaaa catctctcca actccatgct taagccattc
    51001 ttcagatatg ctccaagtct tgcaaatgtc gttcaaagtt ctttcgcttg catttctttt
    51061 accattttca attaaagata ttgtgtttct ttttaaattt agtttattag caaactcttc
    51121 ttggcttaaa ttgagattta atcttagttc tcttattctt ttgttttctg gtttcatttc
    51181 atctatcatt attatccctc catatgaaat attatatttt aaaagtgctt acaagtcaac
    51241 aaaaataaaa gaaaaaggta ttacaaaata gttgacttgt aagcagatat gtaatataat
    51301 tgtattacac aatcaacaaa agcgaggtga ttgaaaatga ctgatataaa taaaattaga
    51361 gaagaaatat atagaaaatt ggagttactt ggccctgcaa aactaaaatt agcattgact
    51421 aaagtagata tattaaaaga aatacaagac attgaagatg aaaataatat acaaaattaa
    51481 aggggtgaaa agattgaaaa tctcagattt taataaaaat caaataggat cagcagttca
    51541 attacaagat ataaggtttg atacaaaagc tgattgcatc attacgaaag tagaaaagaa
    51601 tgaaatacta gtcatgtatt atgaaaaaga aacagaggaa atagcatata aaactttgac
    51661 taaagaagat ctaatacttg atgactataa acttaagtta ttatcttaaa gtaatttcag
    51721 aagggaggtg ataactttgg atcttttgaa ggatgcaaga aaaaaagagt ggttttggct
    51781 ggaaaatgat ttgattgata gagaagattt gagcatctat gaaaaaatga tttacattgt
    51841 cttagctaga tatagtaacg aagaaagttc ttgtttccca agttataaaa caatatcaga
    51901 aaaagcaggt tgcagtataa ggcaagtatc taatattttg aatgaattag agaataagga
    51961 actaataata agagaaaatc gtaaccacga aggcaaaaaa gagaaaaaca gtaacttata
    52021 tttcttaata tcttcaaaag caaaggtaca gaatgaagtg cctaacgtta ggcatgaggt
    52081 gcctaaccct atggcaccag gtgccttaca tgttaggcat gaggtgccta gtaaaaagac
    52141 ttataataaa aagacttata taaaaagtaa taccactaca caaaaagaat ctaaaactga
    52201 ttatttagac ctatcattct tggacttaga tatagaaaaa gtaaaactaa ctaaagatga
    52261 atatgacaaa cttataagca agtttgggaa gaaatacata cacgataaaa ttgttagttt
    52321 agaaaactat atcataaatg gtaaagggag taggtataag agccactata gggctttgct
    52381 gacttggggg aatggagatg ctagtaaagg tatagtacca gaagttacaa agactaagaa
    52441 tcctcttagt ggatttaaag aactttaaaa actaaatagt aaggaggtag ataggatgaa
    52501 tgaaaaagaa gagactgttt atttgaatag ttctaatgtg aagttattct taggcgttag
    52561 ggatattaag gaatttgagg aattgataga aaatgtcaat gagcgaattt gtcaactcca
    52621 agaatcgatt aataaacttg cccaatttaa tattgagttc gatattaagt cagatattta
    52681 gttaagtata aattaggagg ttgcctatga aagatgaaaa gttagaactt actgaaaaag
    52741 atttacactg tattgcaaga catttacaaa atgaagttat ggagatggtg tttagaggga
    52801 atatagaagc ccctacatca tgcgaagttt gcgattactt gcaagaatgc aaggactatt
    52861 tcacccatat agacactttt ataaaattaa gtgaaatgac aggtgtagat atttttacta
    52921 aataaattta gtaaattaaa attttagtat tgtaattgac gtgctttttt aaaaagagga
    52981 cagttgctat tagcagtcat tatttgaatt ttgcaaatgt aatttttatt acattcgcat
    53041 tctaaaagtt ttgatccagt gaatccagga aaagttttat attcaccaga aaattcaact
    53101 ttatcgtcaa taagagagca tttttctctt aaggttttaa ttttgcacat attcatcacc
    53161 accctataat taatttgaac atactgtcaa tatgctccga ttaattataa catgcaaatt
    53221 atcatgatgt agaattaaag cataggtcaa ttaaatttag ttataaaatc aatgaaacag
    53281 ataaataatt aaatgttaaa taaattaggg ggttgttcat gaaagaggaa aagttagaac
    53341 ttactgaaaa agaattgcat tgtattgcaa gacatttgca aaatgaagtt atggagatgg
    53401 tgtttagagg gaatatagaa gcccccacat catgtgaagt ttgcgattac ctgcaagaat
    53461 gtgagggaga ttttacctgt atgtatcatt cttttactaa attaagcaaa ataacaggtg
    53521 taaaagtgac tgcatttaag tattgttttc cagatgaaaa tcccaaagct actatctaag
    53581 aagttttttt agaataggac attcttcatt agaagaagag tcgcaaatat gacttccatc
    53641 acatctagtt gacattaact tagatccaaa agtacctgga ggagctgaat attcgcaagt
    53701 aagagaaatg gtttcgttta ttacaggaca ttctttgctt ataactcgat attggctcat
    53761 actctcacca cctttctaat taatttaaac atattggaag tatgtttaaa ttaattatag
    53821 catgtaaatt atcatggtgt agaatagttt caaatcaaaa gaagcaacta aatagttaaa
    53881 gacaggaggt atgctaagtg gaaagaaaaa aacatgaatt tacagatcaa gatatacaaa
    53941 gacttattga aggattaaga actgatccaa aaataagaga aatgatattt aaaagtaaag
    54001 gttgtgaaac tagaacaagt acaagaagta aatgcgaaca atttgttgaa gaaaaacttg
    54061 cttatagaag ttatggattt gaaactccaa aatactatgc aaaacttatt gccgacataa
    54121 ttgatatttt agtttacaaa aatgttccaa gagatcgtat ttgtggggta ttgtatgacg
    54181 tcgaaagggt gattccttta gttgtgacat tttaataata agctgggggt gattaaatga
    54241 attactcttt agaagcagag caaaacattc taggaagttt tcttataaat gactctatag
    54301 gttacaaaat aagggaatta aaagaaaatg atttctactt tgagtcacat aaaattattt
    54361 tatcttgcat gaagaaaata attgactcaa ataaaccact agatcttttg cttttaaaaa
    54421 atgaattaga aaaaatagat agattagtag atattggagg cgttagttac attaccagtt
    54481 taacaactat agttataact acttcaaaca tagatcacta tataaaaatc ataaaagaga
    54541 agttacttaa aagacaaatt tcagagttag caaatgaatt aattaataat tcaaattcag
    54601 atacaagtat agatgaattg attatagata taaacgattt aaaagcactt gtgacgacga
    54661 gcagtagtgt aaatgataat tacattgacg catcaaaaat aaaacgtgaa aaaggcgtac
    54721 acaagtccat agacacgggt tttaataaac taaacaatat gctagatgga tttagatatg
    54781 gaactcttac aattttaact ggtaaacctg catctggtaa atcaactata gttaaccagt
    54841 ttatagcaca agcaattacg aatggagaga aagcattttt gtattctgga gaattaccct
    54901 ctttcatgtc tatggattgg tttagaaaaa ctgtagctaa tgattatcac ataaaagaat
    54961 ataaaagtgt ctatggagga acatacacag atattccaga ttacgccgtt gagcttatat
    55021 cagactggat agaagataaa ttttttctct atgatgaaga tgcaataagt gatgaagtaa
    55081 atctactaaa taccatagag catttatatt taaaaaaagg ggttaggttc tttgttcttg
    55141 acaatctaat gacaataaaa actggcaata aggcagataa atatgagaga caagagcaga
    55201 tagtaagcaa cttaaaaaat ctcgctaaaa aatataactt agtaatagtt ttagttgcac
    55261 atcctcgtaa aaatatgggt gacatgaagc caactatgta tgatgtgtct ggagcaagtg
    55321 agattgttaa ttatgcagat tatattttat caacttacag agttgttgat gaagaagaag
    55381 agacagacga cacttattta ttaattttaa aaaatagaat aacaggaaaa cagaatataa
    55441 gttttaaaat gaatttttca gaaagaagaa aaagacttta cacgactagt gaggagttaa
    55501 acagagatta caaatatgat gtaaataaac aatatgttca agtagaaatt ccagaagatg
    55561 tattttaaaa aaagtataaa aaataggaaa ttttattaag taatgatttt ataagaaaaa
    55621 ccaatattga ataatttaaa aattcaatat tgaaaaaaat catttttcaa tattgaaaaa
    55681 cggattttga aaaagtattg aatttcaata ctaaaaaaag tttgaaaata tagcatttac
    55741 aaggtttata taaattaatt tcctgacaat gtacacacag gtttagatat gctagcaaaa
    55801 aaacttaaaa gtagtgtaag gataaaaagg ctatataaat ttataaaaag atttaaaagt
    55861 gttattaaat tagagctaaa gtttattaat taatttcctc gtagaaaaat attattttaa
    55921 ataaataaag gagtgatttt acatgatagc attagaaaaa tttgcaggtg gagttctaaa
    55981 agaaaagttt aatacagagt tacaaaaagt tttagataac attgcagatc caaatacaga
    56041 ttttaaaaaa acaagaaaaa ttagtttaga gatagtgttt aaagcaaatg aagatagaga
    56101 tcttgctgaa gtagatataa aatccaaagc aactatagta gaagcaaagg ctacaacaac
    56161 taaagttctg ataggaaaag atttagagac aggaagagtt gaagcgtctg aatttaaaaa
    56221 tcaagtagca gggcagttgg tcatggatat tccagatagt gaagaaacta atgaaacaga
    56281 agaaaatagt gctggtgtaa ttgatttcag aaatgcaagt tcaaagtagt ttaagaaagt
    56341 aagtaaaata taaatttaaa taaaaaagga gaataaaaaa aatgataaga aatgcaatgg
    56401 aatatttagt taagttagga aaaagagaaa cagtatgtgt agatgggttt aagtacacaa
    56461 cagatgcatt agaaataata aaagaaccaa aagctaatga attagaaata actacattga
    56521 gtggtttagt agattatata aaaagtggca tagatcataa ggcagatgag ttgttattaa
    56581 tacaagttct aactccaaga aaagtgcttt taaaatctcc tttgagaaaa aatagagata
    56641 gagagacata tattgaatgt gttgcactac taccagagaa aagatttgac agacctttag
    56701 acttagatag ttttaacata atgctacaat cagcatttat aaagaataat gatagagata
    56761 cacttttaaa aattgcagga aatatccaag aatctacagt aaaaaatgtg ggagatgatg
    56821 gagtttctca gtctgtaaca ataaaaacag gcgtagctag tgtatcagaa gcaatagtcc
    56881 caaacagagt aaagttaatt ccttatcgca ctttccaaga gatagagcaa ccagaaagtg
    56941 agtttatttt cagaatgagt tctggaccag cagcaggatt atatgaagca gatggtggag
    57001 catggaaaaa tcaagcaatg ttaaacatta aagcgtactt gcaagaagaa ttaaaagatt
    57061 ttaaaaatgt aaatataatt gcttaattga aaatctaaga gggcttacaa taagtcctct
    57121 tagtaaaaaa ggaggggatt agatgaaaat gatttctcat atgaacattt tagaaagagc
    57181 agaatttaca gataaaatag ctaatgcaat catgtctgca aataaagaaa ttgaaagagg
    57241 ctgtgccttt aatgaagcag ttgagattgt aaaacaaatg gaatatagag aaaataaata
    57301 caatgaagga gatattgcaa tatgaataat gtagttttag ttggaagatt agcaagagat
    57361 ccagaattaa gatatatacc agagtatggt actccagttg ctacttttgc attggcagtt
    57421 gataggggtt atacaaaaaa ggatggaact agagaagtcg attttatacc aattgaagtt
    57481 atgggaggat ccgcagaatt ttgtgctaat tatctcgcaa aaggaagaat ggtttcaatt
    57541 caaggccaaa taaggataga aaaatatgaa aaagatggag agaaaaaaac tttcacaaaa
    57601 gtaagaacaa aaattgttaa tgcgcttgat cgcaagccaa aggatgaaga aaaagaaatt
    57661 agttttcaag ttttagatga tgaagatatt ccattttaaa ttggatggtg attatgtgag
    57721 aagtttagag gagagaaata aattagttga agaaaatata aattttgtat ggtttattat
    57781 aagaagatat ttcaaagtat ttatttcaaa atatccatat cttcaagatg atacattttc
    57841 agaaggatgt attggattat gtaaagcagc agattatttt gatagtaaaa aaggtattga
    57901 atttaccact tttgcagctg tgtgtataaa aggtcaaata tttaaattta taagtaggta
    57961 tgttaaaaaa cattacagag cagatataga tagttttgaa aaatgcatct ataaaaatac
    58021 tcatggtgaa gaaataagaa tagaagatag attggctggt aatgaaaatg tagatataga
    58081 aaatgttcat gttattagat cttgtataaa aagaagtaaa atagaagata tacagaaaat
    58141 aacatactta agggaaaaag gatacactca aaaagaaata ggcaaaatac ttaggactaa
    58201 ccaaatgaca atatgcagga agcttggaaa attaaaatct gaaattaata tactaggaaa
    58261 ataatataat aatttaagtt cattggaggc aaaatgaaga ctaaaataat tggtggtagg
    58321 gaaaatcagt gcccactttg taatggtagt gtatttaaga ttgaaacatc aattggaata
    58381 gtatggcaat gtaaggattg tggatgtatg tatcaagata tgaattgtaa agaaaacaaa
    58441 aggaggcatt aagtatggct tgtgttataa agtgtgattt ttgtggtgaa acaatagcag
    58501 aaattctaaa cataaaggta ttcagaattg atgatgaagg taatataatg agtatcggaa
    58561 aagatgtatg tgacaaatgt tatgacagat tatttagaag taacttgaat aagaagatga
    58621 ctaattatga aaagataaaa aatatgagca aaatagaaat ggctgaattt cttgcaaatg
    58681 gcggaagtgg atgtactagt tgtgcttatg attttcaaga atgcttaggc ggatgtttag
    58741 aaggtagaaa aaaatggctt gaaagtgagg cgatgaaata atgaaaaaga cgttaataat
    58801 cgtaggagta gttatttcaa tattaattat gtttgcttgc ttgttcttag taactaaaaa
    58861 taaagcaatt gatttagaag agcaggttca atcagcaact gcatctgtag aggtgcaaga
    58921 aaaaaggaga attgatttaa ttaagaacat agtagactgt gtaaaagact atgctaaata
    58981 tgaaaataga acattaaaag aagtaactga catgagagta agtgcaaatg atgaatctat
    59041 caaggaagca aaattaaata tacaagcagt agcagagaag tacccagatt taaaagctaa
    59101 tgaaaattat aagcaactaa tgagtgagtt gagtataaca gaaaatatga ttgcacaaca
    59161 tagagaaaat tataatatgc aagttcgcaa ttacaataaa tatataagaa aatttccagc
    59221 aaatttaatt ctaaatagta tgggatatga aaaaatagaa tctaaatata ctgaatataa
    59281 agcaaaagag gatgcacctc aaaatttatt tgattaagag gtgcacatat gaaatttaaa
    59341 acttttgaaa taactaagag agaattttta gtgagcataa taattgcttg catgatgatt
    59401 gtgcttggat ttttcattga tgataaagtt caatcaaaat ttttagaaga taatgaaaga
    59461 tattacaaag caactaaaat aaataatgac aaagatctct ttgattatgc tatggaaaca
    59521 agtgcgggag attctttgat aagtggaaga tttaaaagtg tagattctgt atctatagaa
    59581 gaattaaaag gggactattg gtttatagaa agaacggaag aaaaatacac tatgcatact
    59641 agagtggtta ccactaaaga cagtaaaggc cattcacata aatatactga aacatattgg
    59701 acttgggatt accatgattc aatagagaaa aaatgtaata aatttaattt catgggtaaa
    59761 gagtttgatg cgaaagtaat tactaattta tctaaagaga gattggcatt aaagaaagat
    59821 atatttaaaa ataataatct taaaataaaa ggtaattatg cttacattaa taataagaga
    59881 aggtactatt atgaagttat accgaaagaa tttaatggta ctatgtatgc taatttaaaa
    59941 aataatacta tagcgaatcc agctgataaa aacataaaag tttttaaggg tgagtctata
    60001 cagaatgttt tagaaaataa taattttaat acagggtttt ttaaagtagt gttttggatc
    60061 atctggatat taataactac aatcattata tatgtatttt attatttaaa aaatgattgg
    60121 cttgaagata aataaagatg taagaggaga agatcaggta tgaaatgtaa ggtgtaccat
    60181 gctattaaaa atataaaaga tgcaaatgag ttaattgata gatttgaaac ggaaaatata
    60241 ttctttgaat taaatgacat agataatgaa aatgtcttaa atggagaaat gaatttaact
    60301 gatatagaag atattgcatc attgcaattt atattagatg gagatttatt tatatttagt
    60361 agttgtgatg aaatgatatt tatgaataaa tacactcaaa aagagttggg acttcaataa
    60421 atgaatgagg ggagatacta aagatggatt taaagttaat tagcagtata cctaattgga
    60481 aggtagaaga tcagttggca aaaattgatg aagaggtaac tgagtttaaa aatgctatag
    60541 atacaggaat aactaaagat ataattgcag aaggattaga tgtttgtcaa actattttaa
    60601 caatgtttca agttttagaa atagaaaact atattactga aggagtagag acacataata
    60661 agaaactaaa tggaagagga tgggatctca aagaaatagg agacgatata cttaatttaa
    60721 ttaaaaattc aatgattaat gaattgatta atatgaacaa gaaattaaaa tgccttgatg
    60781 aaaaagaatt taatgcaaaa atacaatttt tagatgtaga taaactatgg aatttgtgca
    60841 tgcctttagt taacttaagt actataaaag gagttaatga aagcattgat gaaatgaaaa
    60901 tgggagagtt tctaaaaaat attaattaaa gtaaggagat aatataatat gttgatagaa
    60961 gtaactgaaa ataatagaga attaaaagta ggagatattg ttgaaattaa tggtggtaat
    61021 aagttatata tgattctttc tttaaaggaa acaataggtg ggtatgttat tataaatatg
    61081 cagaatggat atggttcatt tggtgcttat aaaagtttaa gtcaactaga attagacttg
    61141 aaggtaagag gttataaatt atatagttct gatgattaca agttgcaatt agtgcctaga
    61201 gaataagata tatttcaaaa aagaaaaaaa ggagcattac ttcacgctcc tgcttgtcaa
    61261 aattctaaaa ccgtcatcac aacatcatta taacataatt aggagtgtga ggtaatgcaa
    61321 tctagtaaaa aagacaattt gtttagtagt gcagaaggta agttatatga ttataaaaaa
    61381 ataaaagcgg aaatagaaaa aatagacatt gatatgcaga tgataaaaaa cgactacaga
    61441 ggatgtagtg gagttgaaat taaagaaaag acaggcaaga catatgatat taaaagtgta
    61501 attgaaatag agactgaaga aaaagaaaag aaaattgcct taaaagaaaa agagaagaga
    61561 cataaagagt tgataataga aaaaatagat aatgcaatga aaatattaag tgaagaagaa
    61621 aaaaagatag tacagtataa atacttttca agtagcagga catcttggga atatgttggc
    61681 agaatgattg gtttttcagc gagtaagtgt aagcaaatga ggtttgatat tatagataag
    61741 ataaaaggat tgctataaaa atgtccgatt tgtgacgatt ttaaaccgat ttatgtctgt
    61801 tttgtgtcta aaaaatgtcc ggtttgtgac caatttatgt ctgttttgtg tcttttaatc
    61861 atgataagat tgtattgtga gaaaaatata tttaataaaa atggcttgga atttacgttc
    61921 taagtctttt ttattgtcta gagaggtgtt gaagattaaa cgtgatataa cagaagaaat
    61981 ttcaagtgct gcatatgtac cagacaatct aaagtattac gacaatgtaa tgaaagaaac
    62041 tcctggtatg tatggaagag aatgttcttt agattttata aagaaaaagc aagaaaaact
    62101 cttgaaacta aaaaagaagg ttaagaaaaa ttatgattct aatattaata agatagactc
    62161 atatctaaaa attttagaga aaagtataat tgatgagtct gatcacgttg agttggttgt
    62221 atttaaatta tatttgcaat tagaaaatgt tgtaaaagta actgaaactg taaacgactt
    62281 aggatttaga ataaagacta acacttatgc aagagaaaga aaatatagta caaatgatat
    62341 aacctctata atcacagatc cttttactga tgtagttgag gacttaaaaa tattagttca
    62401 agagaggcaa agaaaaaatt attatggaaa taaggagtgt ttttaatgaa tggaagcaca
    62461 tagtggacaa aaggttaatc ccataaaaga tatagaggat gtatttagac ttttaaattt
    62521 tctagaagaa tggaatgaaa gaaactattt attagccctc tttggaatgt gtactggact
    62581 tagggtaggg gatatattag cacttaaagt tgcagatgta acagacttaa aactcgataa
    62641 aaaaggaaat aaaataagag tatctaagga ttggattaga gttatcgaag aaaaaagaga
    62701 ctataataga gaggtttttt tatctgatgt aataaaaagt gctatagaaa attatactca
    62761 agataaacca ggagaagaat ttttatttaa aagtggcaag aggaagaaat ttaatagacc
    62821 tatccaaaca agacaagcag gaagaataat aaaaggagca gcaaaaaagg taggaataaa
    62881 agaaaatgtt gcaacacact ctttaagaaa aacatttgca aggcatattt atgatgaaga
    62941 agagaataag acttatgcat tagaacttat aaggaagatt ctaggacata agacaataga
    63001 aatgactaga agatatatag gaatagataa ggatgaagaa gtaaaagcaa taactaaatt
    63061 tactaataag ttaaagaaaa gaaaaagaaa ttaaaatatc ttgtatttta tctaaaattt
    63121 aatatgttac gtttttcaac aacaggacat tattagaatg caaagagcct taacaataga
    63181 gtttacaagg aatagaatag atttattaaa atgtctgaaa atatatatta aaggtcattt
    63241 attcaaggga tttataggga tgtagatatt taaatgttta tttagaagct gagtaaagag
    63301 gtgattaatt ggatgtgaat gaagttgaaa taatcgatag tagctatgaa aaaggtaaag
    63361 agttgacaga agatcagtgc aatatggtaa ctctattgat tacaggtgct acagtaacag
    63421 aaactgccag aattattgga gtaaatagaa aaacaatcta taattggatg aataaagagc
    63481 atgttaggaa agaaatggac agacggaaac aggagctaac aaaccaagga aatttaatga
    63541 tattaaagga tttagatagt tatatcaaca atatcaagga attagctagt gacaagagcg
    63601 ataagagggt tatgttggca gccaatcagt atcttataaa tcgtatatat ggaactccta
    63661 caagtacagt tatacaagca gaggataata gtgttggcat gggtatggat gcaacagaga
    63721 tagaagcagc tatagctaag ataagaataa gaacaagcaa gaagtaatta gatatgaaat
    63781 aaatcctgca agaatatgtt ttaattatgt ttgaaatcaa ttaaagcaaa acatatatta
    63841 tatatatatt tactttaata attaaatcat atattgtatt taactttagt tatagctaat
    63901 ttaataaaat ttagtgagac ttaataatta aagtttcata cagtgaagtg atgtggatta
    63961 ttataggata tgtgaaccta ttggaaatgc taggttcttt ttaactatgc cgaaaataca
    64021 tatttaagga atagttgaaa gataattaat aattctaatc aaaaaaatat ttcttgcaag
    64081 aaaataattc aaaatacttt aatatattta ttttttttat taataggggg tgtccttcta
    64141 ttttgaaaaa attatttttt atggaaaatg agttctagaa attttctagc aaaatttaaa
    64201 aaccagagat gtatttcaaa gagggtgatt ttattagaga aaaatttaca ttgtttattt
    64261 tattaattgc attactcact gagcctaaaa aaatagaaga agtaagagga agtaaatacg
    64321 agtggataat ctagaaagaa atatacaatt aatatttagt tatctagcaa agacttttat
    64381 aagaaatgga gcatctataa aagatgctga aaaagaagca aatgagatta taaaagccaa
    64441 ttcaaacaat ctttggggag ttaatggtct cgcttatcaa cttggaaaaa taaacctaga
    64501 atttttctgt atgtatttta tgcaagacac atatttacca aaagaagata atgctgctgc
    64561 tcctatagct aaagttcatc atgagctatg ggaagatata caagaatcta taattggaga
    64621 tggttcacag caattaggaa gaatttatcc aagagggaca ggtaaaagtg catttggaga
    64681 tttagcgaca actgtatggt cgcattgcta taaacataag acatatactt tgatttgctc
    64741 tgacatagga tctacagcag agaaatttgt aaaagatata aaaaatgcat tacttgaaaa
    64801 tgagtatatt aaaaaagcat ttggtgtcct tttaaatgat aatgatagaa aatacatttg
    64861 taatagtact caactagagc taacaaataa aacttttatt gaagctatat catcttcatc
    64921 acccatgaga ggaagaaaat acaataataa ccgtccagat cttatcatac ttgatgatta
    64981 tcagtcagaa gaaaatgtta gaacagaaga tgctagagag aaaaaattta aaagattttc
    65041 tgatgatgta aaatatgctg ctcaaaaacc agtcataaga aatggtaaaa ctataaaaag
    65101 aggaacaact tttatagcat taggaacctt acaacacaaa gaatgttttt atagtagact
    65161 aaaaaattta ccaacttgga agtttaaatg cgaaaaagga gttttagttg ataatgtaga
    65221 tgaattattt aattctggtt tatggcttaa gttcaaagag atcttatttg attttaaaaa
    65281 tacaaatcat cttgaagatg ctaaagaatt ttactgggaa aatgaaaaag aaatgaaatt
    65341 ccctatatta tggccaagtt tttgggattg tttagatatg gctctaagtt attatgaaaa
    65401 tccaacatct tttaagcagg aagtgcaaaa tgatgtcaat tctataggag aaaaatggtt
    65461 taaaactgtt agaacagaaa ctagagaaga aattgaatcg catactttta aaaagacaat
    65521 gctgttatgt gatccagcat ctgcgggtgg gtcgaaacat gactatagcg cttttcttgt
    65581 tggaagtgaa tcagaaaatg gtttactata tggaagattg gcagaactag ctaaaataaa
    65641 tgctagaact gattttgata aatatataga ccatatgatt tatttattaa aagtgtattc
    65701 agatataaca catatttata tagaaaaaaa tacatttaat ggcgcagatg ccaaccaatt
    65761 agagtttaaa ataaaaaatg ataatgttct tagttataga gatatagaaa tcattaatga
    65821 gcaacaaaag aaaaataaag atgataaaat ctctacttta atacctgttt taagcaaagg
    65881 ccaaatgatt tttgcagaag aagataagga atttatacag caaatacttg attttacagg
    65941 acaaaaatac tctctacatg atgatgctcc ggatatatca tcggaattta ttaatcgaat
    66001 taacaacata aaagtttctg agacagtaaa tatctttgat agaaaattat taggttttta
    66061 gttgggaggt aatttattgg ctaatagatc aaagtttgaa gaagttcaaa aaactttaac
    66121 taaaaaagga aagaagttca atgttggaat aggaaaagat gaaaaaggat attttgcata
    66181 tactcataga gcaaggagta aaagttatat ttcaaagcaa gatattccaa taaaagtttt
    66241 aaaatttata gagtctacag gataggatgt gttgttaatg gaattagatt tagacttaat
    66301 aaaagacata tatgacgatt ggaactctaa taaatccgaa tatgatacta tgtataagta
    66361 ttataaaggt gaaacggatg ctattagcaa ctataaaatg gttaccaaga gatctaataa
    66421 taagataaac actaatttct taaaaaagtt tattaatgaa gaagtcgctt attctcttgc
    66481 aaataagatt acctatacaa gtagatctgg tgatgagaag attataaatg atttagaata
    66541 ctatacttgc cattggagta aaaaacatga ctcagattta ttgaggtatg cactattatt
    66601 tgggctttgc tatgaacttt actatgtaaa agatgatgaa atacaagtaa agataatcaa
    66661 gccaacagat ggctgtcatt atgaaaatga aaatggagaa atagtatgct ttattagaga
    66721 gtttaccaaa ggtttcgaag atgacacgta tgtagatgta tatgacaaag aatatatata
    66781 tcattttgat tcaaatttca aagaagttga aagtcctaca gtaaacaata tatttgatgg
    66841 aaatgttcca atatcaattt gcaaaagaag tgaagaacta ggaaaaaata caatatttaa
    66901 tgatataaaa gggttacaag atgcttatga aactaattta agtgatataa gtaatgaaat
    66961 tagtgatttt agaaatgctt atttaacctt ttctggatgt aatattaaag aagatgattt
    67021 gccaaggatg aaagaacttg gaatacttca agttaatgga gatggaaaaa tagagtggct
    67081 tattaaagat atgaatgata catttgttca aaatacacta tcttcaataa aagaaaatat
    67141 gtatgaaatt acatctcata taaatcacaa tgaaaaaatg caaagtaata catcaagtct
    67201 agcagttata gcaagattaa tcagtacaga atggatttgt agccaaaata atgatagtat
    67261 tgcagatact ttatttaata ggtacaaatt attgtgcatt tggcttaata aaaaatatgg
    67321 ttttgactat gattataaag atattaaggc taaatttacc cctaagatac cacgtgatga
    67381 tttagttgta gcaaatatat taagccaact tggagataaa ttatctactg aaactggctt
    67441 atctcaacta agtttcattg ataatcctca tgctgaaata gaaaaagcta aaaaagaaca
    67501 ggaaatagtt tcagagggag agattttatt agatgaatca aaagattata actaaattga
    67561 ctgaagatat atataaatct gctgaaaata ggacaaaacc attttataag tatcaaagag
    67621 aaaataggga taaactttta ttagaaatag caaagatatt gctaacacac gatattatta
    67681 atgaatactt aaatattaat aataaagata agaaaaatct aaggtcaaaa cttaataaaa
    67741 ttataaatga gtattctaat agttatgatg aagaagtcaa ggatatagaa actattttat
    67801 ctgattcatc taagaataaa tattcaaatt tgataatttt attaggagta atactgaaga
    67861 taaaagatgt tagtaatata aacaataaag ttataaataa gatagtgaat aataaggttg
    67921 atggaaaaca ttggagcgat agactctgga agaataaaaa aggtatagaa gagtccttaa
    67981 aaaaagaaat aaagagtttt ctaaatggaa aaacttctgt aaataagata gaaaaagcaa
    68041 taagagataa atattcaaca ggtgcaggac aaaccagcag actaatagaa aatgaagttg
    68101 ccagatgtca aagtgaagta aatgagtatt ttttcaaggt tcaagggatt ataaaagtaa
    68161 tgtattgtgc agatttagat tctcgcacat gtagtgattg ttctatgcat aatggagtga
    68221 tattttatgt caatgaagat aggccctctt tgccaagaca ctgtcactgt agatgtcaat
    68281 atatacctgt ttaacatgta ttaattaatt taataggtgt ttttattatg tctaaaaaat
    68341 atgaggtgaa atatgaaagt tgaaatatta ggaacagaat atctagtgat agagaaactt
    68401 gaaagcgaag atgtgctatt aaaggaaaga gctggatatt gtgatcactc tgttaaggaa
    68461 attgttatag agaaaataaa ttctgaagaa ggttcattga aagatttaag tgtatataaa
    68521 aatgaagttg ctaggcatga aataattcat gcatttctta gtgaaagtgg attgaagagt
    68581 tgtagttctt gggctacaaa cgaagaaatg attgacttct ttgcaattca atttcctaaa
    68641 atagtaaagg ttatggataa aatagaatgt ttgaattaaa ttttcaaaaa ggagaacttt
    68701 atacatgtta aaaaaagaag tgttggaatt actaaaagaa atagaagatg atgcagaaat
    68761 agattcattg ctgcaatcaa cagatttatt caaatctgca tctgataaaa agcttacaat
    68821 agaagagttt agagaattaa ttaataatga tgcaaacttt aaagctatta tagataatga
    68881 aaaaaataaa tatcacagcg aagcacttga aaactttaag aaaaaagata tgcagacact
    68941 tataagtgct gaagttctaa aaagaactgg agcaaatgaa acagaagagc aaaaagcaat
    69001 aagggaatta agagaaagcc tagacaaact aaaaaaagaa aagcagtacg cagaaaaagt
    69061 ttctaaatat aaagatattt tagttgaaaa gaaaatacct acaaacttaa ttgaatatct
    69121 attatctgat gatgatgata aaacaaatgc aaatatagag atatttgaaa attcaatgaa
    69181 gcaatatgtg cagtcaaagg tagatgaaag aattaaagat ggttcatacg ttccacctgg
    69241 caaagatagt gcaggttcgc tatcagaaat tagaaaacaa ataaagcaag ggctaaatag
    69301 tctttagtat aaaaaaagaa aggatgattt tataaatggc taatgtatta caatatgtaa
    69361 cactttttca acaagaacta gatatgcagg taacagttgg ctcaactact ggatggatgg
    69421 agaaaaatgc tggtcaagtt atatataatg gaggaagaga ggtaaaagta ccaaaagtaa
    69481 caatggatgg cttaggtgat tatgacagat ctaaaggata tacgcaaggc gcagttacac
    69541 ttgagtacga aacaatgaaa atggagatgg atagaggtag gacttttatg ttagattcaa
    69601 tggatgtaaa tgaaagtaac ttcgttataa atgcatcttc tgtaatggga ttatttcaaa
    69661 agcaacatgt tataccagaa atagatgcat atagatactc aaagatattt tctgaaacag
    69721 ctaaagtaga tgagcgtgta aaatattcat acgaagctac agtaactaca atattaagtg
    69781 aattgaaaaa tcaacttgca tcaatacaag atttatctgg agatatacct cttattatta
    69841 gtatgtctag cataattgca agcatattag aacaatcatc tcaacttgat gatattgaat
    69901 ttggcgatgg agatataaaa agaattgtaa agtctataga caatgtacct ataataaaag
    69961 ttccatcaaa aagaatgaaa acagaatacg tatttagaga tggtaaaaca tctggacaaa
    70021 cagagggtgg gtttactgaa ggagcaaagg ctttagatat aaactggtta ataactccag
    70081 catctgctcc aattgcagta tctaagactg ataagccaag aatatttact ccagatcaaa
    70141 accaaacagc agatgcttat caaatagact atagaaaata tcacgatttg tggatattgg
    70201 atgaatattt aaaactttgt agagcatctg tgagacaagc taaagctgca caagaataag
    70261 gagttgtttt aaatgtttgc attgataaaa aataacataa ttaaagttgt tgaagatgaa
    70321 aattcgagag ataaattgat ttcaaaagga tttaaaatat attcagaaga agtgactaaa
    70381 gagaatattg agaaaaaaga agaaaaagtt aaatcaacct ctaatgattt atttaatcta
    70441 tctaaaccag agcttatgga aattgctaaa ggcaagaata taaagggata ttcaactaaa
    70501 actaaagagg aattaattca actactagat gagtacaatg cttgataatg ttaaattagt
    70561 tttaggaata gaaaatgata gatatgacaa attaataatc ctttatataa ataaaataag
    70621 tgagatggtt ttagaatatt gttctaggca ggaactaagt acagcactag aagggtttgt
    70681 agaagaaaaa gtaatatcaa tattatcttc aagatttgaa gaacttaaga aaaatagtgg
    70741 acttgataat ctagaaaaaa gcttaaaagc tataactaga ggtgacacta agatagaata
    70801 taatgcttta acattacaaa ctgctgaaaa tagcatcagt tcatcatctt ttttaactga
    70861 tactgataaa aaatttctaa gcccattttg tattgttagt atgtactaag gggtgattaa
    70921 atgactgaag cagatatatt agaaatgaca taccttgata aaatgactat aattagaaaa
    70981 ttaaaaggct ggaatgaaga aacatgttct aatgatttta gtaatgttgt tatagctgaa
    71041 aatatcccat gtgcaatttc tagaaaagaa gaagccattg taaatggtga tattggatct
    71101 atagttgtaa atagaaagtt attttgtaga ccagaagtag atataaaaat aggtgatact
    71161 ataaatacaa cctataatca tggagaagaa gctgtattta aggtttctaa ggcaaatata
    71221 tatccaagcc atatggaaat acctattaca gaattggaga ggatataatg ggaatagata
    71281 atttaggagt ctttgaagca ttagccttag atttaggtct aaatgcatca aactttaata
    71341 gaaaaatcaa gaagtttaca aaagaaactg ggcaagaact agcagaaaat ataagagatg
    71401 aaactcctgt aaaaactgaa aagttaaaaa agggatggga agaaaagcaa ccagaatatg
    71461 atacttatat tgttaaaaat gaagttgatt atgctacttt agtaaataat ggccattcaa
    71521 ctaaaaatgg tggcattgtc cctggttctt ttatggttga aaagggaatt gaaagaacaa
    71581 aagaaaatat gacaagtgca aaagcaaatt tatttgattg gtaggtgatt agttgcttac
    71641 aaacagagac attataatag cagttaataa aagaatcgaa aatgagttta gatatataga
    71701 tatttttatt aatgaggata atatacaagg ctttgaggag gcttgttttt ttgtgcaact
    71761 tttacctgtt tctaataata ttgcaaataa agttttagat actaaaaaga tattcataga
    71821 tattgaatat taccaaaagg ctagaaaaaa taaattaaat ctatacgaca tacaaagtag
    71881 attagaaaaa atatttaaca gaaacattag agttaaagat agaagtttaa tcattgagaa
    71941 tatatctcca tcaatcgcaa aagatcctgt aggatataaa ctaacttttc taatgactgt
    72001 atcttatttt gaagaagtct attttgaaaa tgaaacattt gaaattatgg aaagtgtaga
    72061 tttagagata ggaagtgatt aaatgggttt accagaaatt aatatttctt ttatccaagc
    72121 aggaaaaact tttgttaaac gctctggtgg tattgtagcg cttattttaa aagatacaaa
    72181 aaatataggg ttagttgaga taaaagaaat agaggatatt ccagaaaatt attctgctgc
    72241 taatttagat tatataaaaa tggctatgaa gggaaatgta tatcctccaa ataaagtatt
    72301 agtttataca ttagatacag atgaatcaat agatgatgca ctagattttc ttgaaacttg
    72361 tgagtttaat tatctttgta tgccagatga gacagaacaa gatttgccta aaatcaaaac
    72421 gtggattaag aaaatgagag aagataataa aataaaggtt aaagctatta ctgcaagtaa
    72481 ttcagcggat tatgaaggta taataaactt tacagcaact gatattgaag ttgaaggtaa
    72541 aaaatacact tcaaatgaat ttctgccaag aatagcaggt tttattgcag gaactccatc
    72601 aactcaatct gtaacatatg cagaaatacc agaagttact aatataccta agctaacaag
    72661 agctgaagca aatacaagga taaataatgg agagctaatt ttaataaaag aaagtggagc
    72721 tatagtaatc gctagaggag ttacatcctt tacaacaatt actgattcaa aaggtgatat
    72781 attcaaaaag attaagttgg ttgatacact agatcaaatt cataacgata ttaaaaaaat
    72841 aattgtaaaa aattatatag gaaaaaccca aaacacttat gataataagt gtttacttat
    72901 tgttgcaata caattatatc ttcaagaatt agaaaaagat ggtttaattg atgaaggttc
    72961 aactgtagaa attaatttag atgctcaaag agcatggcta aaaaaacaaa atatagatat
    73021 atctaatatg gaagaacaac agataaaaga atacaatact gacactcttg tatttttaaa
    73081 agcaagaatt aaattaatag atgcaatgga agatgtatat ttagacatct ctatgtagtt
    73141 tggaggttaa aatttgaaag ctaaaataga tgcaaaaaga ataatatctg gtacttttgg
    73201 agagtgttgg ctagataata agcaacttac atcaacaaaa gggatggagg ccaaggttga
    73261 tatagataaa gaagaaataa aattatgtgg tcaaataatg accacatata aaatgattgg
    73321 tgcaactgga aaaggatcta taactcttta taagataagt accttatttg gcgagtatgt
    73381 cgaaaaaatg ttaaatgaag gtaaagaatt taaagtaact ataataagta aattgcaaga
    73441 cccagattca tatggttctg agagaattgc actttatgga tgtatgtttg acgacttaac
    73501 tttggctgat tgggaagctg cgaaagttgg agaaatagaa gttccattca catttgaagg
    73561 ctttgaatac ttagataaaa taaaagaaaa ataggaggat tttattatat gaacacagta
    73621 gaaaaattat taaatatgga tgcaggtaaa ttaaaaatgc catctagcgc atttgaatta
    73681 tattgtaaaa aactagatga tactttagaa atagagtgca aggctattga tccagaacgt
    73741 tttgacgaaa taagaatgaa ctctatggac gttaatagtg gagatgtaga aaacattaac
    73801 atatatgaac ttaaagtaaa tacaatatta gaatcatgtt ctatatttag caatatggaa
    73861 ttagttaaaa aatttagtgc aattacacct aaagaattaa ttaaaaagct attacttagt
    73921 ggtgaaatag ataaattata cgaggaggtt aataaagtaa atggaataga tgcagaggga
    73981 gataaaaaaa gacaaaaaga aagagctaaa gatataaaaa actaattaaa acggatggag
    74041 aagttaactt gatgtactta ttatttaagt ataaaggtat aatgccatcc gtttcttatc
    74101 atatactaag aggcgaaaaa gaaattgtta gtgtttttat ggatcaagaa ttagatgaaa
    74161 gaaaagaaga aataaaatct ggtgttggga ggttgtttta gtatatgagt tctaatacaa
    74221 aggtcctaca agcaataata aaaatgagag atgaagcaag ttcaacatta aaaagagtag
    74281 cagcaaatac tagagctcta catgattcat cttcccaagc atcaagagca ttaaataatg
    74341 ttcaaagtaa tttaaaaagt gttggaacta gtgctttaaa agcaggaggt cttatcactg
    74401 ctggagttgg tggtgcattt gcagctgcaa ctaaagttgg aacagaattt gaaagcgcta
    74461 tgtcgcaagt taaggctaca tttggaaatt ccttaaaacc aggagatttt gaagctctgt
    74521 ctaaggctgc tagagatgct ggaaaagcta caactaaaac tgctgctgat tctgctaatg
    74581 cacttcagta tatggggctt gctggatggg atgttcaaaa gtcaatgcat gccttaatgc
    74641 cagtgcttaa tttatcagaa gcagcctcta tggatcttgg tctagcatca gatttagtaa
    74701 ctgatagcat gagttccatg tgcttagaag ttgataaggt cagtaaaagt ggaaaagtac
    74761 tagctggtgc aggtttaagt gagtatttag ataaagttgc aaatgcatct actaaatcaa
    74821 atacaagtat acaacagttg atggaagcgt ttaatgtcgc aggtggtaca tttagcaatt
    74881 taaaagttcc actttcagaa gcaactgcta tcatgggaat acttgcaaat agaggagtta
    74941 agggtacaga tgcagggcat gctttaaact ctatcatggt aaatcttaca acaggtgcag
    75001 gtcaagcagg taaggcaatg aaaaagttag gtgtaagtgc atttgataat caaggtaaat
    75061 ttaaaggtat gtcaaatgtg cttaaagagg ttgcaaataa aactaaaggt atgactgatg
    75121 aacagaaaaa ttattactta tctgctattg gtggaaagac tcaattaaaa acgttacaaa
    75181 agttacttga tggtgtggga aatgaatatg atagcttaaa gaaaaaaata gataattcaa
    75241 aaggtgcttt aaaaacaatg tctgatgtaa tgaaggataa tactaagggt ggacttgcaa
    75301 ccttaaaatc agcccttgaa gaaactggaa ttactattta ctatcaacta aagccagcaa
    75361 tagcatctgt agttgataat ttaactaaaa tgactgtttg gtttaataat ctaagtgaac
    75421 caataaaaag agtcataact aatgtaatgc tttcagttgg agcatttgga gtacttttat
    75481 taactatagg ggctgtgtct ttaattgttt caaaagctat aggtatattt ggcttttttg
    75541 ttccaataat attaagagca aaagttgctt tcagagcttt tgtaattggt atgaaaccat
    75601 taagtgcagt atttttagct gtaatgggtc ctgtgggatg gactataaca gctgtaattg
    75661 cattatcagt agcatttacc attgcttatg ctaaatctga agtatttaga aataaggtta
    75721 attcattagt aaaatcattt acagaatttg caaagcctat agtaaatttt tgtatgcctg
    75781 cacttaaaca gctaggagag aaatttaaag agttgttatc agcatttggt ccactatttg
    75841 catctatttt aaacctagga agtgtaataa tgaaggtact aggacctgtt ttaatcggcg
    75901 taataggttt tcatatagca aaattagttt tgattttatc tactctggca actgctttta
    75961 aagctgtagt tgatactata actaatgtat taaatggtct agcatctatt ttaaatggag
    76021 tatttgattt aattataggt attgtcacag gcgatagtaa aaagatgatg gatggagtta
    76081 aacaaatatt tgatggtgga actaaaatta taaaatcaat ttggaaaggt ttcatagatt
    76141 ttgtaactgc tcctatacaa gctatagctg atatagctga cagcttattt aaagataaag
    76201 ctaaatggct tacagataag tggagagatt taagaacatt cttagaaaat ccaattgaag
    76261 caactgccaa aatatctact aaaacaaatg taaatatccc aggaagtaaa gcagcagatg
    76321 cagctggtaa atctacacca aatacaggtg gattaaaaca aacagtagca aatctaaaag
    76381 gttcatttag tgattttaaa acttttatta agcctatgac agactctata agtcaaggag
    76441 ttgaatctgg aaaaactaaa tttacagaac tgcaaaataa aatattaaat gatgttaaac
    76501 ctgcactaaa ggtcctagag gattctggag taaattttaa ggataagttt aaggagtcaa
    76561 taacttcaat aaaagataaa tttaatgaat taaagaacat attagctgga aatattaaaa
    76621 caaatataga taatttagta aaagcatttg aacctttaaa acctcattta gataatttga
    76681 aagattgctt tgataaggtt aaaaaagcga ttactgattt ttttactcca gccaaacagg
    76741 ttggagatac agtaaacaaa gtaagccaac caatggatag tgttaaaaag tctactgatg
    76801 gaattaaact ttctttccaa agtttaactc aagcacttgc tcctgttaaa gcttggtttc
    76861 aaggatttat gcctttccta tcacaaatag gacaacaact tttaccatct ataggaattg
    76921 caatagctgg tgttgttggc ggaataattt tagcatttac atgggtttta aatacattga
    76981 ccgcaataat aacaacagta tctggaatca taaatggaat tatgtctttt atatctggaa
    77041 ttattaatgt tattatgggg cttataacag gaaatgctga tcaaactatg gatggactta
    77101 aacaaatatt tgatagtgga gtaggtgtaa taaaatcaat ttggaatggc cttgttgatc
    77161 tagttagtgg cgtactagaa cctgtaatag atcttgctga ttctcttttc aaagataaag
    77221 ttaaaggggt aaaagaagct tggaataaac ttaaagattt ctttaaacat ccaattaagg
    77281 gaacaataga aatatttaaa aaaggtccta gtttacaaat tggaaaaggt gcagatgctg
    77341 ctggcaaaag tccaagaaaa gcatttggta taaatagagt tcctagaaat gattatttaa
    77401 taagagcaca cgaaggcgag aaacttttaa ccaaacaaga agctaatcaa tacaaaagga
    77461 atagttcaaa tggaagttta tttcttgata aattggctga tacaataatt gtaagagaag
    77521 aggctgacat agataaaatt gtaagaaaaa taaacaaaaa aatatctctt gcaaaaattg
    77581 gaggtgtttt gtaaattatc gtgctataat tgtagtatat aatttacagg gggttatgtt
    77641 atgaataaaa aaatactagt attaacaata tcaattatac tatctttaag tttagttgga
    77701 tgcagcacaa ataattcaaa aaatagagat agtaaatcta agcaaaacac agaggaagtt
    77761 aaaaatcata aaataggcga agttgttgat ttagatggag caaaagtatc tgttaaaaat
    77821 attaggaaat tagaaacaaa tagtaatgat tatgctgttg aaattgaaat aactaataca
    77881 ggtgataatg atttacaaag tgattatcta gaaaactata tattaaaatc aagtgacaat
    77941 actactggag aagcaataaa taaccaagaa ttaaatggag agttaatgtt tgatacaata
    78001 cgcccaggag atactttgaa aggtgaaata gcattcgaac ttacaaaaga tgcaacacca
    78061 aaaacacttg aaattgctta tgaaaatgta ggcaaatata cagtatttga tttatagtaa
    78121 ttatcttaaa agacgcttat ttacttaagt gtcttttatt ttaggaggag tttaatggaa
    78181 atatggttaa gacaagcaaa taatacattt aggtttcctg ttattccacc ctcttttgag
    78241 ataaatggaa gtgcgacaat aaatacatct aatattttaa gtgttggaga tatcgcagta
    78301 tttggaggat tagggcttaa aactatagaa ctatcctctt ttttccctaa tcaagaatat
    78361 agtttctgta attataatgg atttccaaaa ccatatgatt gtgtaaattt aatagaaagt
    78421 tggatgaaag aaggatatat attaagattt atcattacgg aaactaatat aaactttgaa
    78481 tgtataattg cagattttaa ttatagagaa caagattatt caagagatat ttattttacc
    78541 ttaagtctaa aagaatacag aaggatacaa atatccaaag taaacattaa taatgatgaa
    78601 aaattatctt ctgaaaagaa tgttcctcta acaaaaggat ttgatactaa gcaaaaaacg
    78661 cataaggttg ttgaaggaga tactcttttt aagatagcta aaaaatatta tagcaatgga
    78721 gatttatggg agaagattta taaagcaaat gaagatgaga ttaaagatcc atctgtaata
    78781 aaaaatggtt gggtattaat aattccttaa aaatggtggt gatttataat taataaaatc
    78841 aaaatacaag ttcatataaa aaacggaaat atatataatg taactgattt agtggataaa
    78901 tgcacttggt ctggtgatta tagatcacca tcaagaactt tggaattttc tataattcaa
    78961 tctgcaagtg atattaattt taggcagata gatattccag tagcaagtac agtttgtttc
    79021 tatgtagatg ataaagaact ttttagagga atgataatag atagatctaa agactctagc
    79081 aataataata tagattttac agctaaagat atgggttttt tacttttaca aagcgaagtg
    79141 tcatacaatt ttaaagataa attagtcgaa gatattgcaa aacaggtttt tgtagataat
    79201 aaattcccac taggtaattt acctaaaact aacgttaaat atacaaaaat gtttattggt
    79261 gtaactggct acgatactat aatgagcgtt tatacagaag caagcaagac aactaaaaaa
    79321 aagtatatga tagagtctaa tcttgataaa tttaatgtta ttgagaaggg tgtagttact
    79381 ttaaatataa catttgaaga aggctttaat cttatcaata caaatttttc tgaaagcatt
    79441 gagaatgtaa aaaataaagt actagttgtt gatcagtatg gtaataaaat tagtgagaaa
    79501 ataaatgact ctatatttaa agacgttggg gtaattatgc aaaaggtaat acaacaacaa
    79561 gaaaacagta ctatagacat agatggagag tttaaaggaa tcgaaaaaac ttgctcttta
    79621 aaaggatatg gtgatataac ttgtgtaact ggtagaggtg taaaagtaaa agactcatat
    79681 acaaaattaa taggtctttt ttatatagat acagataagc atacatggca aaatggagat
    79741 tatcaaattg aactagagct taattttgag aatataatgg atgaaaaatc tgctggccaa
    79801 gatgagcaaa aagaagatgg ttcagagtat actggaggaa cagaataccc tgctgaattt
    79861 acggcttatt gtcctagaaa agaagagggt ggaaatacag attgtagagg taaaaaacta
    79921 gatccttcta aaaagacatg tgccgctcct atggttggag catatgaaaa atcttactat
    79981 acaaaagatt tcttaaacaa acatcctctt atgagatatg gagatgaaat acaattagtt
    80041 acaggtgttt caagccgaga tggaacgtat aaagtaaatg ataatgggcc tgcaataatt
    80101 atagaaaagg atggaacata tcatatagat attttatttg gaaatgttga agaagctaat
    80161 aactttggaa gaagaaaagg aaagattata attggtggtt attctggaaa catgacagaa
    80221 aaagcaaaaa cagtaatttc agaagcaaga aaacatcttg gaaagccata caaatggggt
    80281 gggaatggac caagtagctt tgattgttct ggactaatgg tatattgctt taagaaggtt
    80341 aatgttaatt taccaagaac ctcaagtcag caatctaagg taggtaagaa agtagagaaa
    80401 aataatttac aggcaggaga tttggtattt ttccataatc cagtcagcca tgttggacta
    80461 tatgtaggta atggagaatt tttacatgcg ccacaaacag gagatgtagt taaaataagt
    80521 aaattaagta atagaaaaga ttttaataca gctagaagag ttttgtaaaa gaggtgatat
    80581 cgtggcagat ccaatcaatg aatttatagg aataattagg caagaaggta aatttcataa
    80641 tgaaccttct ttttttattg gaaaaattaa aagtaaatta ccagatttaa aaatagagat
    80701 aaataacatc atattagaaa aagaagatat tttgatagat agttggatgc ttgatagaca
    80761 gatagagtta tttgatacag aaacaagtca agaacataaa catgaaataa aaaatccctt
    80821 tatagatact tttgaatctg gaaacacagt aataatgttt aaaataggtg aaaaatttgc
    80881 tgttgtaagt aaattggtga gtctagatga ataatactat attccctttc atgggtgttc
    80941 cagaagatta tatttctcca gacaatgaag aattacctat ttttaaagaa tttgcttggg
    81001 attttaataa agatgaaaag ataattgaaa atggagactt taaaatagtt gaaagaaatg
    81061 aagcaattaa agtatggatt tacaaaacaa taaaaacaga taagtatgtt catttaatat
    81121 atgattggag ttatggaaca gatataaaaa atctaatagg gcaaaagtat actaaaggtc
    81181 ttactgaaag tgaggcaaaa aggtatatac aagaagctct attaactaat ccatatatat
    81241 tagaagtcaa tataacaaat gcagaatttg aagatgataa tttatctata agtttaaatg
    81301 taaaaacaat ttatggagag gaggagatta cttttgtata atggccaatc attcagtacc
    81361 ttaaataata gaactttaaa taacataaat ctccctcttt ataaaggcca aggatcttct
    81421 ctttataata tagtttctcc aataaattca gaacttgcac aattatatat agaactttct
    81481 tatatacata agagagtttt tattcaagat aattttgacg attttcttga tagaagagtc
    81541 aatgaatttg gcgtatatag aaaattaggt acagaggcaa taggtgaggt aacatttgag
    81601 ggcaaaattg gaactcaaat accaaatgga acaataatat ctcatagtga tttactattt
    81661 gtaattatta aagatattac aatagatgaa aatagtaaat taaatgtaag ccctatacag
    81721 gctttagaga ttggcattaa gtataattta tcagctaaca cagaatttaa acttatagaa
    81781 gaaataactg gagtaactaa aatttataat gaacttgacc taaagggtgg aacagaaata
    81841 gaaactgatg aagaattaaa agaaaggttc tataaaatac aaaaaaatca agcgacaagt
    81901 ggaaataagg cgcattacca atcttgggct ttagaggttg aaggtgttta taatgcaaaa
    81961 gtaattcctc gttgggatgg tccaggtaca attaaaattc taatttatgg tcaaaacaat
    82021 caatcagttg acaatgaagt attgcaaaga tgtactgaac atatagaaga agaaaaacct
    82081 attggaccta ctgttacaat tgttacacca agtacttttg atattaatat tagtgcaact
    82141 ttaacacttg aaaatggata tgatattgaa tctataaaag ttgtgttttt agatattata
    82201 aattcttatt tgatagaaaa ttcaagagag attgtttata taaaagtaat gagtttgtta
    82261 gcaagcatag aaggtattca tgatataaaa aatctccttg taaatggtga tgttaaaaac
    82321 ataatagttg atgaagaaaa agttcctgct gtttcaagtg ctatatttga tatcgaggtg
    82381 agttaaattg aagttaattg attacctgcc aagttttgca aacaatgaaa ttgacataca
    82441 aatccaagaa gcacttgaaa atgaactttt aacgctaatt gatgaaaaag atgatttatt
    82501 agaacagttt tttatagata cagcaacttg gggacttgat gattgggaga accttttaag
    82561 cataaaagta aattataagt tagacttcga tactagaaga agcaatataa aagcaaagat
    82621 gagaggtaag ggtacaacaa caatagaggt tataaaagct atctcggaag cttacacaaa
    82681 gaccaatgtt gatgtagaag tatttagtaa tctatttagt tttacactta gttttataac
    82741 aaataattgt agttataaca ctattttaga attagataag aaaatagaag aaataaaacc
    82801 tgcacacctt gaacacaaat tcgagaggat attatttaat aaaaacgagc tttatacagg
    82861 tgcagcaatt agtacaggag aaacagttac aatatatcct tatgtaccta gaaatttaga
    82921 aagttttgga gaaatagcta tttgtagtgg aaatgataga gcattagaaa aagtaacatt
    82981 gtatcctaaa aaatagaaat gaggtgataa aattgacaga acaacaatat tttactctag
    83041 taactgacat tggtaaggca gcaatagcaa atgcaagtat tacaggtgaa aaagtagatt
    83101 ttgcaaagat aaaagttgga gatggaggag ggagttctta tactccaaat gagagtcaga
    83161 cagcactaaa aaatgtggtt tgggaaagta cacttgaaca tgcacaagga gataaagata
    83221 atcctaactg ggtagtaata caaaaattca tacctggtga tgttggagga tttgaaataa
    83281 gagaagttgg tctatttgat tctaaagacc aattattagc gatttctagt tacccaacaa
    83341 catataaacc tactgcggat tcagggactg taaaagaact attaataaaa gtaatattag
    83401 ttgtatctaa tgtagctaat attaatttga aagtagaccc aaccgtaatt ttagcaactt
    83461 taaaagatat acaagaatta gacactaaaa tagatacaac taaaacagaa ttaacaagca
    83521 acatagaaac tgctaaaaca gagttgaaca ctaaaatagg agatacaaca caacttacta
    83581 caacagataa aacaaatatc gttagtgcat taaatgaggt aaaaactagt gtagatagta
    83641 tagaaacaac agcagataaa acaagtataa aagatacaga taatttattt gagagcgaca
    83701 acgtagaggg agcattaaaa gaagtgatgc aagaagtaaa aggtaataga agcagtatta
    83761 tatcaacaat aaatagtaat ttaataccaa tgtagaaagt gaggtgataa agtgaagata
    83821 tggaaataca atagaactgt gaagaaaaga agaggtgtct atacagaatt agatatactt
    83881 gcacaaaggt atgaatttga agcagatggg aaactttata cagcttattt tcatttatat
    83941 actaataaga atatagatat attcagagat aaagatttat ataaagaatc ttatatgatg
    84001 agctatagag ttggaaatgt aacaactgat attgatgatg tagtacagca aacaactaca
    84061 gtaagattta tacctgaccc tgttattgat attgtgattg ataacattag cagaggatgt
    84121 gaaataaact acagaattat agataatgaa ccaagtgtaa aattcattgt tacagaaaag
    84181 ttaaatggca caataataga aacaaagaca aattctagtg ataataatta taaacttaaa
    84241 ttaactgatg aacaaatagt accattagca ttcaattccc aaaattcaat agtaatagaa
    84301 ataagtacag aagatggtgg attagtaaca agtaaaacag taacatttac aagaacaaat
    84361 aacaagccaa caattttagt taattcatat agttctaatt ctgcaaaatt tacagcatct
    84421 gatttagata ataatttaag taaaattgag tggtatttag atgatgtatt aaaagaaaca
    84481 ataacaacag atttaacagc agaaaaaacc atcaactatg aatttacaga caatgcaata
    84541 cacacattaa aaatagttgc tacagatgca gaaaatgcaa cagttgagaa ggttttaagt
    84601 ataagtaaag agataatgcc acttcaatct gatgctagtt taagtgatat atcaactaag
    84661 ctgatagaga ttggagaagg gtttagaaat ggtaaaacaa gtattataaa cactttagca
    84721 cttaaaaata tagaagcaag tttaaataat acacttgtgg agttatcaga gaaaataaaa
    84781 acaagttttg atagttcaga cgctagtgtg caggatttgc aaaatagaat aacagaattg
    84841 aataatcagc ttagtcgaag aaaaaaatgg gctacaggta gatacacatt tactagagaa
    84901 gaagatacta tcaatttcag aatggttgat ggagatagtc tttccgcagt aaaaaaacta
    84961 attataccca ttaatttaga ttttgttcca agtgttatta taatagacaa aatgttttta
    85021 ggtagttacg tacctgcaca agatgtgcaa tttaattgtg tttcaaactt agccgatttt
    85081 acacttggac gtcgtttcta taataaacaa tggatttgca cttcttctat aaaactagat
    85141 tcagttacac aaaatagttt tactctttct ttacaaaaag ttgttccatt tgataaagtt
    85201 agtagtacga ttttttctat tattaatact ggttttgctt ggagagcata tgaataaaaa
    85261 tataaattag aggtgattaa atgaatagaa ataatagaat aatttacgac caaacaggca
    85321 agatatggct tcaaactggt gaagcaatag gagatataca agagttgtca gaaataactg
    85381 aattaaattt catagatgtt gaatttggta gtatagacta tagtaaacaa tatatagagt
    85441 ctataaatcc agttacaaaa gaattaaata taaaaaatat agaagttgtt ttgactgatg
    85501 aacaaaagag attagaagta ttagaaaaag aactaaatat gttaaaagaa gaaaataaaa
    85561 atagagattc tgagatagta aatacagcat ttgaagtagg aaatatcaaa ttaaacaata
    85621 atttatagga ggaaattata tgtataactt attaaagtta atgatagaac aaaaaaacta
    85681 tagcactaag gaggatttac aacataagat ggatgtattc tatgcagtaa ataggattac
    85741 agaagaacaa tatttagagt taacaggttt attaaataaa gaagaaacac cagtagaacc
    85801 aacaatatag gttctttttt attgaaagaa ggtgactaaa tgacttttaa agaattagtt
    85861 agtaaagtta gaaatcttgt attagaagca aagaatgtaa ctatagaaga tacagagaat
    85921 aaattcacta gtgaaaatgt cgagggagca ttgaaagagt gtatagatag agcagatttg
    85981 gcttttcaaa gtgccgatag tggaaaagag ttaattgcaa ctgctatcgg ctctcctgct
    86041 acatccgaac aaacatttca agagtatgct aattatattg caggatttaa aaatactata
    86101 agtcagttaa attctaaata taaatatact agtggaactt ttacctatta tactggtgca
    86161 tatcctaatg ggctaaatat aggttttaga ccagatatta tttcctatac tgccattaat
    86221 gctgtagagg aaaaatatta ttgtgtatac acacctttat tctatctttt ttataattat
    86281 acaactggag aaaaatatct tgatgttaat cataatcgtt atggaataac ttttactgac
    86341 aatggatgga tacctagaaa tccagttaac aatggtgtgt atagttatat agcagctaag
    86401 tgccgttagt tgtcaaagac ttagataaat ttctaagtct tattttaata caaaattagg
    86461 aggaagtatg aacgtaacaa tagttttttt agcaacaaat atatttataa aattagtaat
    86521 attagcaata gcatttgata cattgctagg ttgtctaaga gcaataaaaa cacatcagtt
    86581 taatagctct tttggaataa atggaggaat aagaaaagtt gcaatgatag catgtatatt
    86641 ttttctagca gtagttgaca tacttacaaa gtttaacttt ttatttatgt tgccacaaga
    86701 ttgggttgat ttcttacgac taaatcatct aggaatatct gaatttttct ctattttatt
    86761 cattctatat gaaagtgtaa gtatattaaa aaatatgtat ttatgtggat taccagttcc
    86821 taaacgatta aaagagaaaa taggtagttt attagatact atgacagatg aattaaatgt
    86881 taaaggagga aaataaataa tgaaaatatg tattacagta ggacacagta ttttaaaaag
    86941 tggagcatgt acttctgctg atggagtagt taacgagtat caatacaata aatctcttgc
    87001 accagtatta gcagatatat ttagaaaaga agggcataag gtagatgtaa taatatgccc
    87061 agaaaagcag tttaaaacta agagtgagga aaagtcttat aaaataccta gagttaatgc
    87121 tggaggatat gacttactca tagaactaca tttaaatgca agtgataggc aaggaaaagg
    87181 ctcagaggtt ctatattaca gtaataaagg tctagagtat gcaactagaa tatgtaagaa
    87241 actaggtaca gtatttaaaa atagaggagc taaattagat aaaagtttat atattttaaa
    87301 cagttctaaa cccactgcaa tactaattga aagtttcttt tgtgataaca aggaggatta
    87361 tgagaaggct aagaaacttg gatatgaagg tatggctaag ttaattgtag aaggtgtatt
    87421 aaataagagt attgaaagta atgaggtgaa gcaaatgtat aaacacacaa tagtgtacag
    87481 tggtgatgat agagtatcag cagatatttt aggattatat tataagagag caaaagaaag
    87541 ttatctagtt atagatataa aagactataa accacataga acacaaaatc tatatgtaat
    87601 tggtggagta acttgtaata aaatgaaaga aatgagtaag actacaggag aaaaatttac
    87661 tcaactatat ggtaatgatg tatggtcaac aatggataaa gttatagaat ttgtaaaaga
    87721 aaaattataa attaaaagac aacctttaaa aattaattgt taataaaact aataaaattt
    87781 tcaactcaga aggagcatta tatgttaaca tttttagatt tgtttgctgg cattggaggt
    87841 tttaggctag caatggaaag agcaggacac aaatgcgtag gccattgcga atacgataaa
    87901 tttgcaaact taagttacaa agcaatgcat aacccaaagg aggatgaatg gtttgaaaaa
    87961 gacattagag aaattagagc agaaaatatt cccaaagcag atgtgtggtg ttttggattc
    88021 ccatgtcaag acattagcat tgcagggaac aaacttggat tcaaaggaaa tcgttcaagt
    88081 ttatttttct cagttacaaa acttattaga gaacttaaag aagaagatag acccaagtac
    88141 ttacttattg agaacgttaa aaatctactt agtgttaata gaggatttga cttcgccaaa
    88201 cttctcattg agttggatga aatcggctac aatgcagaat ggcaaaatct caattctaaa
    88261 aacttcagag ttccccaaaa cagagacaga atatacattg ttggacatct tagaagaaga
    88321 agtacaagag aagtatttcc tatcacagga acgagtagaa aaactactct tgagcaatta
    88381 aataatccta atcacagttc aaacaggatt tataattcaa atggattatc aaagtgttta
    88441 ttaagtaaag gtgggaacaa tacaggtctt tatagagttg gcaatatcaa tcctagtgga
    88501 aatggtgcag gcggacaagt gtatagtagc gaaggctttt ctcccacact tacaactgga
    88561 aaaggtgaaa gcccaaagat attaattaaa gagaatacta aaaaaggata catcaaagca
    88621 tctataggag atgggataag actagatcat atagaaggta aaactggccg tggaagagtt
    88681 cagcacaaaa aaagttgtac tttgactact gcaagtaatg ctggcgtaat tggtaaaaaa
    88741 tttagagtaa ggaggttgac tccaaaagaa tgttttaggt tacaaggatt tccagacgat
    88801 tattacgata gagcggcaag tgtctgttca gaaactcaac tatacaagca agcaggcaat
    88861 agtgttactg tagatgttgt tgaagaaata gcaaagaaat taaaatagaa tattaataca
    88921 atgcaaaatg tggtaggttt ttccttccgc attttgcatt gcattagctt ttttggaata
    88981 acttacctgt actatcttaa ataaaaaaat gctactatat tataaacatt tacaaggagt
    89041 gcataatgtt aatacaaagc ttaaaacgtg gcgaattaat tgtaatgaaa atcttttaga
    89101 aaagaaattc aagaatatca aagaaagaaa ttattcgcat ttgcaatgaa aaacacaatt
    89161 ggcggaaatc tgcgacaaaa actctactta aaagacttgt taaaaaaaga gttctactta
    89221 agagaattaa aattttccac atatattaca tacctatagt tacagaaaaa gaatatttca
    89281 aatataaaat aaatgattta gagccagatt atttagatga atattttata cgattaatgt
    89341 ccactgtaca taaatttaaa aatctagatg aagaagatta aatatctccc ttcatctact
    89401 cttcacatat tttaatagac tttcaagcaa taattgactt aacaaatcat gcttatcata
    89461 atgtttattc tcatctgcaa atttattaaa catttcccac actgtattat ttattctaac
    89521 tgtagttctt tttatagatt gatctggaag atctatagat atatcatttt ctttaatgac
    89581 acatatatga taactatcat ctttagtttt aaaccattcc agcacattct ttatattatc
    89641 aatttcacta gataaatatt taatatcgtc atcaatctta ctgctaatgc tatctatatg
    89701 atatttatca tcataacttt tattatcttc tacaaacttg tttccagttc ttttatatcc
    89761 tttcttatta agccacttcc ttatagtact ttcatttgcc cctaattcat ctccaacttt
    89821 tttaagactt tcaaattcac ttaatttatt attaatacaa tcaacaactt cattaatatt
    89881 tttttctttc aaatcactcc aagtcatata aatcctcctc aaaaaaataa tagattacaa
    89941 atatcatata tttgtaatct attatacata ctataaacta aatccttttt tatttcatca
    90001 tttttttgta tccttttgca tttgcaaaaa catgtttatc tacatcactc ataactatta
    90061 tgccaggtat attcttttta aatgcttcac ctaacaattt agcgcctcca cccataacaa
    90121 caaccgcagc tgtactaact ggataattta atttcaattc attgtatatt tctttaaata
    90181 gagaaataga tgcatctata tttttcttta tatcttgttt ttcaccattt aggtaaaatc
    90241 ctttatttat gtagtcttgt attttttctc tttttatctc aactttacct aaaaatcttt
    90301 cgtccatctc aagagatttc tttattgcat catatatatt tattgttccc acatttatag
    90361 aatatggatc agtcgcttta ttcccttgta caagcgcaat atctgttgtc tttccaccaa
    90421 tatcaactaa aacaacatct cttcctgcaa tttggtcaac tatgtctttt gcataatata
    90481 agtatcctgt aacaccttct ggaaatgttt gaatagatct tattgttata tctcttttag
    90541 tagtctcatt tcctcttgta aactcaacat gatattcttt tccttgtaat ttttcaataa
    90601 gattttttct taatccggca aattgtttca ctggcagacc tagccctaga tcaacaattt
    90661 catcatctgt atttctacat atagccccta atagtagtgg taagaagttt tctttctcat
    90721 atttaactat atttatttcc tcgtcacctt cacctaggta atatgtttta ttgcctattt
    90781 taatgctatc tgaatctgaa ccaaagtttt ttacctctgt aactttactt tcaaaaatat
    90841 catctgtact tgttttaact gcataatttc caatatctat acctaattta ctcatttatg
    90901 ttatttcccc cttattattc taaacttcca aaatcaaaat tgttagctgg atcttctatt
    90961 tcttttttct gttcaactac agcattatcc tttttttctt gcattttttc ccaaacaagc
    91021 tcttttaagt aagctgtagc tgataatttc tcatctaaaa acttttcaat tgctttttct
    91081 ttatcattgt ttttaaatgt tagaactatc ctactcattc aaccacttcc ttttttataa
    91141 aatatta
//
LOCUS       DAMJYR010000002        86355 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.002, whole genome shotgun sequence.
ACCESSION   DAMJYR010000002 DAMJYR010000000
VERSION     DAMJYR010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 86355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 86355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..86355
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.002"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            39..155
                     /gene="rrf"
                     /locus_tag="PC471_000108"
     rRNA            39..155
                     /gene="rrf"
                     /locus_tag="PC471_000108"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            275..466
                     /locus_tag="PC471_000109"
     CDS             275..466
                     /locus_tag="PC471_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HDF2787257.1"
                     /translation="MKKVALILAIIGALNWGAIGILGTDLIGSIFGGTYEMVSRIIYF
                     IVGLAGLYLIPSLMSDDRE"
     gene            621..2030
                     /locus_tag="PC471_000110"
     CDS             621..2030
                     /locus_tag="PC471_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HDF2787258.1"
                     /translation="MFIINELNKIVNEEVISFHMPGHKKGQVYKKLGYKDILKNLYKM
                     DTTEILGTDNLHFPEGIIKNSQKKASRVFKSDYTCYLVNGSSCGIQSAIMSVCSPKSK
                     IITNRDCHQSVINGCILGDIDIEYVPCEISEETNVLKGISISNVIDIIDKNLDAKAVL
                     LTYPTYYGMTYDLEYICNYAHSKKMVVIVDEAHGAHLGLSERLPKTALEQGADIVVQS
                     THKTLPAFTQSSMIHIQGDRVDIDKILNMLRITESSSPSYLLLSSLELAVDIYENKGK
                     ELMEELLNNIITFKNKVDSNINIYDTNDKTKIFISSKNMGLTGYELENILRNKYNIQV
                     ELSNYYGVLLICTIGNTVEDFSSLETALNNIVKEDFKFEKLENKKYPVVIPKKILTPR
                     EAFYKTKKSVKIYDSIGKVCGECIVPYPPGISVISPGEVISKEIIDYLMFCHSKGMIV
                     SGLKDVDLNFIQIIDLEDI"
     gene            2161..2850
                     /locus_tag="PC471_000111"
     CDS             2161..2850
                     /locus_tag="PC471_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HDF2787259.1"
                     /translation="MKGKLIIIESGSDASGKATQTEILYERLKKEGYMIKKVEYPNYE
                     SPSSALVKMYLKGDFGKNASDVDPYIASTFFTADRYASFKTDWEEFYNEGGIIIADRY
                     TTSNMVHQASKMDICDREEYLNWLADYEFNLFKIPQPDCVIFLDVPIEFSKKLMENRK
                     NKITGEEKKDIHESDIEYLEKSYNNALYIADKYDWKKINCVLDNKLRSIESIHNEIYK
                     IVLDSIKPMER"
     gene            2853..3788
                     /locus_tag="PC471_000112"
     CDS             2853..3788
                     /locus_tag="PC471_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HDF2787260.1"
                     /translation="MYFENIIGQDFAKKYLTNSIKKNKLNNAYMFEGMDGIGKKKFAD
                     ELSKLLLDYENLENSPDYVLIKPDGNSIKIAQIRNLQSDIVIRPHKDYKIYIINNAEK
                     MTVEAQNALLKTLEEPPNYAIIILVTNNKESLLETIKSRCDIIKFSPIPIEDLKRYLI
                     NTGIEEERAQLLAIFSRGSIENALNLSQSSEFSMMREDIQQYIQIMLDKNIVEILNIP
                     NNMEKYRGKIIALLDMMINYFRDIILLKENVNKNMLINVDKLVFIQNMSGKISYSQLS
                     KIIDIIEDAKSKIKSNCNFNISIQVMSLNIYEVIK"
     gene            3785..4678
                     /locus_tag="PC471_000113"
     CDS             3785..4678
                     /locus_tag="PC471_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HDF2787261.1"
                     /translation="MIKIVGIRFKSAGKIYYFDPVDFNIEQDMDVVVETARGLEYGKV
                     VVGPKDMDESELVSPLKPIIRIATEEDKKVYLENKEKAKETFELCQQKIKEHELTMFL
                     IDCEYTFDRNKLIFYFTAEGRIDFRELVKDLAAIFKTRIELRQIGVRDEAKSIGGLGP
                     CGRKLCCSSWLGDFQPVSIKMAKDQSLSLNPTKISGICGRLFCCLKYEHDVYSEAIEK
                     MPVVGALVQSDDGKGKVVEVNPLLEQIKVEFQDKTVKIYTREDIKILREPKKCDGCGG
                     KCKDNDGLDEATLRELKKLED"
     gene            4756..5502
                     /locus_tag="PC471_000114"
     CDS             4756..5502
                     /locus_tag="PC471_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HDF2787262.1"
                     /translation="MQLKDSERIDDLQLKGLKIIQDTNGFCFGIDAVLLANFTKIKKD
                     AKVVDLGTGTGIIPILIAGKSEAKKIIGVEIQEDVYEMATRSIKLNGLEERVEIINED
                     IKYIDKVLDINGYHVVTSNPPYMHIDGIKNPNDKKAISRHEVRCNLEDVIRAASRLVM
                     PRGKFFMIHRPTRLVDIITLGRKYKLEPKVIQFVHPRPQKAPNLVLVQFVKDGRPELK
                     ILDPLYVYGEDGNYTKELKAIYNNKDIGEL"
     gene            5503..6336
                     /gene="rsmI"
                     /locus_tag="PC471_000115"
     CDS             5503..6336
                     /gene="rsmI"
                     /locus_tag="PC471_000115"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HDF2787263.1"
                     /translation="MSGKLYICPTPIGNLEDITYRTLRTLKEVDLIAAEDTRHSIKLL
                     NHFEISKPLTSYHEHNKDSKGDYLINKLIDGENIALISDAGMPGISDPGEEIIKQAIQ
                     NNIEIEVLPGATAFVTALVGSGMDTHRFVFEGFLDRDKKVRKAQLEELKEESRTIIFY
                     ESPHRLKDTLKDMLKILGNRNISINRELTKKYQEIIRENIETTIKIFEEKEIRGEFVL
                     IVEGFHGEKSKKENYDGLTDREYVLKLIESGMNKKDAIKIVCKDRKLKKDVVYKQVLD
                     L"
     gene            6359..7063
                     /locus_tag="PC471_000116"
     CDS             6359..7063
                     /locus_tag="PC471_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HDF2787264.1"
                     /translation="MEIGKIIIESLKREDTHKIFIELDNKHELEKIIPKISCMKSVGE
                     CKYHVVNCFEHSINALKELEIVLNDKDFFPIHLRKHVSNYLNTYIEEGINKLHVLKLG
                     TFLHDIGKPDSMTLDETGRVHFTNHEKIGAQIIDNMGIKLELSTETYRLISKYVRYHM
                     ILLSLYKKNDLSRKELINVFNLVDEDTIGVIMLGYADIVSTVKLLGKSGEVSVLKTYM
                     EYILTNYLYKSNYTHV"
     gene            complement(7251..7679)
                     /locus_tag="PC471_000117"
     CDS             complement(7251..7679)
                     /locus_tag="PC471_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007287587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HDF2787265.1"
                     /translation="MDVTDYRIIEILQDDGRISMKDLGKIVGLTSPAVSERVKRLEES
                     GVIEGYKAIVNPDSLGRVIKAFIHISLPSNGYTEFIESAAKDPRIVECHHITGDDCLL
                     LKVIVKDMYELENVIDTIKKIGSTKTSVILSTPIQAKSIL"
     gene            complement(7716..9146)
                     /locus_tag="PC471_000118"
     CDS             complement(7716..9146)
                     /locus_tag="PC471_000118"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HDF2787266.1"
                     /translation="MRNLTLLTDLYQLTMLNGYFEKNIHEDIVVFDMFFRKNACDGGY
                     TIVCGIDQVVEYIDNLHFSDEDLEYLKNLNLFSDKFLKFLKEFKFTGDIYAVEEGTIM
                     FPNEPLITVKAPLYQAQLIETALLTIVNFQSLIATKASRVCFAAQGDPVFEFGLRRAQ
                     GPDAGIYGARAAVVGGCAGTANVLAGKMFDIPIIGTQAHSWVQKFDNELEAFQAYADV
                     YPDKCLLLVDTYDVLNSGVPNAIKVFKNISEKGYKPMGIRLDSGDLAYLSKEAKKQLD
                     NAGFSDISITASNDLDEYTITSLKAEGATINSWGVGTKLITSFDSPSLGGVYKLAASC
                     EKGVLEPKIKISENPEKINNPGYKKVIRIYNEDNKAEADLIMLHDEVIDESKPLEIFH
                     PTYTWKTKVFTNYKVKELLKPLYIKGRCKYNKKAVLEIKNHVQYELSTIWEQYKRLSK
                     PHIYKVDLSRNLWYLKTQMIDSKKVL"
     gene            9278..9862
                     /locus_tag="PC471_000119"
     CDS             9278..9862
                     /locus_tag="PC471_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore maturation protein A"
                     /protein_id="HDF2787267.1"
                     /translation="MIIIGKIWFYMVSIGIIGSLISKNMGELNKVILSETSKGIEFAI
                     SLAGIMALWMGIMNIAKESGLIEKIGQKLNPIMRRLFPSIPKGHKAMSYIVMNIALNM
                     LGAGNGATAFGLKAMSELQTLNNKKDTATNDMIMFMVINISSIQIIPFTIIKLRMDMG
                     AQNPSEIIFTTLFATIISTAVAIITCKFFQRRYR"
     gene            9878..10399
                     /locus_tag="PC471_000120"
     CDS             9878..10399
                     /locus_tag="PC471_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore maturation protein B"
                     /protein_id="HDF2787268.1"
                     /translation="MEIFSNLLIPIIILYIVIYGKYKKIDVYDSFVKGAIDGLKAAWD
                     ILPYIIGIFLAIGIFKTGKGLDMLEWLFTPIANMMSIPKELIGLISVKPLSGSGALGM
                     YSELANRVGIGSLVEKMGATIVGSSETIFYTMAIYYGSLKIKNTRHTLSCAMISHVAG
                     VIAAVFICYVIFV"
     misc_binding    10465..10711
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            10795..12732
                     /gene="metG"
                     /locus_tag="PC471_000121"
     CDS             10795..12732
                     /gene="metG"
                     /locus_tag="PC471_000121"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HDF2787269.1"
                     /translation="MSKPSFYVTTPIYYPSGGLHIGHTYSTVAADTIARFKRFCGYDV
                     KFLTGTDEHGEKIQKKAIEQGMSEIEYLDGMIKDIKALWNTMDISYDDFIRTTEKRHT
                     DIIQKIFTKLYEQGDIYKGEYEGRYCTPCESFWTESQLLEGNKCPDCGRETYLVKEES
                     YFFRLSKYEDRLKELFKDDSFCFPAARKNEMVANFLDKGLEDLSVTRTTFDWGIKVPF
                     DEKHVIYVWVDALCNYITALGYMTDNDEEFKKYWPANVQIVGKEIVRFHTIIWPALLM
                     ALGLEVPKQVFGHGWILFADDKMSKSKGNVVYPEPIIERYGIDTLKYFLLREFAFGQD
                     GSYTHRNFVTRINYDLANDLGNLISRTVAMVEKYNNGIIPTAKVSTDFDADLKEQAVS
                     TRENFEAEMDKMQFHEALESVWKLVRRTNKYIDETMPWALAKDETKKDELDTVLYNLC
                     ESIRIIATLINPIMNETANKIYEHIGIKGQDDITTWESTKTFGLIGENVKVFKGEPLF
                     PRLDVEKEIEELTKMFSGKPPVEEKPLEHKEEITIDDLDKIELRVGKIISCEKHPKAN
                     KLLVSQVKIGPETRQIVSGIAEYYKPEDLVGKEVTVVCNLKPVKLRGVESQGMILAAG
                     DDGDPYVLPFTQGAKDGCEVR"
     gene            12762..13532
                     /locus_tag="PC471_000122"
     CDS             12762..13532
                     /locus_tag="PC471_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HDF2787270.1"
                     /translation="MLFDSHAHLNDESFDEDRDELIGSLKDKGVDLVVNPGADIETSI
                     TAIELAKKYDFIYSAVGVHPHDVSKLDDTAIETLRKLATENEKVVAIGEIGLDYYYDY
                     SPREEQKEWFKKQIELANELKLPIIIHDRDAHGDTFEIIKNTKNPEIGCVLHCYSGNV
                     ELAREYVKMGCYISIPGTVTFKNNKKTREVVREIPLERLFIETDSPYMSPEPHRGKRN
                     NPSQVSFVADKIAQEKGISYEEVCRVTKENAKKFFNIK"
     gene            13870..14301
                     /gene="phnG"
                     /locus_tag="PC471_000123"
     CDS             13870..14301
                     /gene="phnG"
                     /locus_tag="PC471_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnG"
                     /protein_id="HDF2787271.1"
                     /translation="MYTVDSTKILINMNLEKLEKIYSMIKDTSSISIIKEPNLATVMV
                     RANESVKNTTFNLGEILVTECSVKVDESLGYGIVSENNNKKAIYLAVIDAVLHSNNSK
                     FDELKNYINKSVYEENLRYEQEIIDEFSLINKTKVQFNAMD"
     gene            14316..14891
                     /gene="phnH"
                     /locus_tag="PC471_000124"
     CDS             14316..14891
                     /gene="phnH"
                     /locus_tag="PC471_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnH"
                     /protein_id="HDF2787272.1"
                     /translation="MVNYTQEIYRKMLDSASFPGKLNNIDNINIENNTNLLNGAIGIA
                     ITLLDQEVTFYIENYTQKDIKNIRALTMSNQIDYRKSDYIFLDINSEVDILQIKIGSF
                     EYPDESATIVHQVNDLSTYSQTKYIKLDLSGPGIKSKNSIYINGIREEFIERLSSINK
                     DYPIGIDLILVDKKGEIAFIPRSSKLSWEVL"
     gene            14894..16009
                     /locus_tag="PC471_000125"
     CDS             14894..16009
                     /locus_tag="PC471_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-phosphorus lyase complex subunit PhnI"
                     /protein_id="HDF2787273.1"
                     /translation="MAYVAVKGGEEAILQAKNILEFYRVKGDSLPIDVKQIEEQMSYA
                     VDKVMSEGSLYNKELSALAIKQAQGDILEASFYVRALRSTMPRITYSEPINTEEMRIK
                     RRISATFKDIPGGQYLGATRDYENRILNVELLEEDEKKNRHFRDSFINELETVDEIPE
                     FTKISNIFRKENLLQEEVCENKLEEVIDDITRNSLRFPVSRAAKLQALTRGESGVVSG
                     FAYAIVRGFGDEHPYISELRTGNVFIKIKHPIYEDEEITIGDMLVTECEIVSKASQNK
                     TENSKNNSEDKNKMSLGYGLCMGNNENKAISMAILDKSLEPNSEKLAQDEEFVLLHID
                     GIDSLGFISHFKLPHYVDFKADVERIKKFEEVSLGDI"
     gene            15999..16868
                     /locus_tag="PC471_000126"
     CDS             15999..16868
                     /locus_tag="PC471_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate 5-phosphate
                     C-P-lyase PhnJ"
                     /protein_id="HDF2787274.1"
                     /translation="MIYNYGFLDENTKKEIRRKILKAISIPGYQVPFGSRELPIAKGW
                     GTGGLQLTLSLIGKNDVVKVIDQGSDDSTNACNIRSFIASVSNVETTKDTLEATLIQT
                     RHRIPEEKLKSNQILIFQVPIPETLRIVEPSEVETRRMHSEEDYSRMWVYLYEDIVRF
                     NDISIAVEYPCKVNDRYLMNPSPIPRFDIKKLNMSDNLFLFGAGREKRIYAIPPYTKV
                     EPLEFEDYKFEEEKFEGKYCSLCNSTNTFLDEVYDSDTNEKYYSCSDTSYCEKVRLKN
                     NSIDVTIGGTWNE"
     gene            16861..17727
                     /locus_tag="PC471_000127"
     CDS             16861..17727
                     /locus_tag="PC471_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2787275.1"
                     /translation="MSKINDSKRLENIDKQVLIGRNINLIYGDGCEKCFESTGYESNN
                     ICKYCGSIVACNNVNLELYEGEVLGIVGESGSGKSTLLKILFFQEHANSGEVYISHYN
                     KDTNILELSDQKKRYIKNHFMGIVYQNPHLGLNLDFSSGGNIAEKLLMANLYNVEKIR
                     DRGKELLIKTNIPVERIDHKPKYFSGGMQQRVQIAKALSNNPPILLLDEVTTGLDVSV
                     QAEVLDLIREIQREFKISMIVVSHDFDVIKMLADRTIVMKNGSVIESGLTDQIMEDPQ
                     HPYTQQLINSLL"
     gene            17768..18481
                     /locus_tag="PC471_000128"
     CDS             17768..18481
                     /locus_tag="PC471_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDF2787276.1"
                     /translation="MKETILEVNNFSKDFELHALNKTITACSNISFEVSKGEFLGIIG
                     KSGAGKSTILKSIYKTYIPTTGEIIFNSEIYGNVDLAKIGDREMINLRKKEIGYVSQF
                     LKTLPRITAIELVVHSLIESGFEKDCSYDMAKDILNQFEIKENLWDAYPNNFSGGEKL
                     RLNLAQAMVKKPRLLLLDEPTASLDNQSKIYVKEKMLELKSQGTTMIGIFHDIEFMET
                     VIDKTFTMTKGTISESGVA"
     gene            18482..19651
                     /locus_tag="PC471_000129"
     CDS             18482..19651
                     /locus_tag="PC471_000129"
                     /EC_number="3.6.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate diphosphatase"
                     /protein_id="HDF2787277.1"
                     /translation="MIKLIHNANIVLEDMILENGHLIIEGDTIKKVSNDKIDYAFDFN
                     EIDEIIDAKNLYVIPGIIDIHSDAIEKEIEPRPSTLLPFNMAFYELDKKLPANGITTV
                     YHSISLGDGVGVRSIDNSLKMIKNIDSYKNIDSKSINHKVHLRYEVLYYEGLEKVLEL
                     LDENKIDYLSIMDHSPGQGQYTNPTFYKEYATKVWGVTENYVDTWLDDLVNLHDNLDW
                     NKIANIIGIAKTKNINVASHDDDTLEKMDFIKNIGINVSEFPITLEVAKHSKKLGVLT
                     CLGAPNIVRGKSHNNNLKAMDAILEDCCDIVCSDYLPSAMIKSMCIVAERINNLNKAV
                     SLFTSNPAKAVGIYDERGSIKENKKADLVLVDIDSEYPKVVNTIVNGKTVYKREV"
     gene            19655..20491
                     /locus_tag="PC471_000130"
     CDS             19655..20491
                     /locus_tag="PC471_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HDF2787278.1"
                     /translation="MLTELHCHTSISDNNFTTEEIIKKAAEKNINILAITNHDTLSGL
                     DEALVLGEHYGITIIPGIEISAYDYKNRKRVHILGYNIDLNSQEIKNLCNPMVCDRHK
                     ASVKMVNKIIDLGYKISLEDVKKYSSKTGIFKQHIMKALIDKGYTDNIYSSLYKELFH
                     RGNGKVYVSLKYVDYRDAIKAIKNSGGICVLAHPGQMDNFSAIEEMVKVGLDGIEVYH
                     PSHNKELEKESLSYAKKYNLVVTGGSDYHGFYSQHNYELGSKSLNKEDLFKFRLALGG
                     VL"
     gene            20492..21487
                     /locus_tag="PC471_000131"
     CDS             20492..21487
                     /locus_tag="PC471_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDF2787279.1"
                     /translation="MSFLQVKNVGKSYGQVKVLKDISIDIEKGEFICLLGPSGCGKST
                     LLRIIAGLEDKHGGKIIINDKDMTNSPPESRNFGIVFQSYALFPNMNVYKNIAFGLEN
                     KNISKSNIDKKVKEVLEVVELSGYEKKYPSQLSGGQQQRVALARAIALEPDFLLLDEP
                     LSALDAKVRLKLREQIRSLHRKLGITTIMVTHDQEEALCLADKMVVMNRGEIIQVGTP
                     KEVYKNPETPFVADFIGTINFIDDGINKIAIRPEDIKVESNRDSKDKDIKVGEILDIE
                     FRGFNYRITVEYRSKQMKLDVVSKVAEQMKLCIGSKINFKIPKEGIVQYKSEGCA"
     gene            21505..23193
                     /locus_tag="PC471_000132"
     CDS             21505..23193
                     /locus_tag="PC471_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     permease subunit"
                     /protein_id="HDF2787280.1"
                     /translation="MITSKNKRRISDSTIQKYFLIFSVIVLIVFILFPLVSLLKNAFM
                     DTSGNFIGISNFSKYVENPSLIASFKNSIFVSTVSSLISLCLAFIYSYAITRTNIKFK
                     EFFKTMGMFPLFAPTMLYGISLIYLFGNKGIFTTMGLEVPLYGPLGIIISQILFTFPQ
                     AFLILSVALSMADYRLYEAADSLGASEFKKFCKITIPGIKYSLLSSFFVSFILAFTDF
                     GAAKVVGGNYNVLATDIYKQVVGQFNIPMGATVSMVMLIPVLIAFTLDKMASKKQGMT
                     ITAKSMPYKIKENKFRDRVFLIVCSFITLLIFILIFVSVIGSVIKLWPYNLSFTMEHY
                     TFKGILGSGIKTYLSSLKISTLTAIFGTIFVFFSAYMIEKVDKFPKLRQTAYLLSMIP
                     MALPGLVLGISYITFFNSASNPLNFLYGSTAILVIVNVVHFYSVCFITSNSSLKMLDK
                     EYELVAKSINIPFYKVFFNITVPMSITSILEIVVYYFVNSMVTVSALIFLYTPQTQTA
                     SISILKLDEIGYIGPSAAMAVLVLLTNIIVRLLYEFVTKKLKNNTQKWQQKDVA"
     gene            23205..24272
                     /locus_tag="PC471_000133"
     CDS             23205..24272
                     /locus_tag="PC471_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HDF2787281.1"
                     /translation="MKFKKLVSLGVLSTLLLSVITGCSTDSSGSDSKSDSKTKGDLTV
                     YTAIEEDSIEPYLATFKEKYPDIKLNIVRASTGDITARLLAEKDNPQADVVWGVAATS
                     LLVADDQGMLEPYAPKGSEEIESAFKDDKEVPSWVGIDAWMTGITVNTKELSDKNIPV
                     PSSYEDLIKPEYKGLISMPNPSSSGTGYLTVSALIQIMGEEKAWQYMDKLHENIGVYT
                     QSGSAPATSAASGEYPIGISFGYRGIKLKEEGYPVEVVFPKEGSGWDIEANALVKKDN
                     IKEASKVFLDWAISKDAMNEYSKNYAVTTISTGNPIPEGFPKKPLEQMIDNDLKSAAK
                     NREDILNKWISKYDGKTEKES"
     gene            25036..26103
                     /locus_tag="PC471_000134"
     CDS             25036..26103
                     /locus_tag="PC471_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HDF2787282.1"
                     /translation="MKFKKLVSLGILSTLLLSVITGCSNVSSGSNLKSNNKTKGELTV
                     YTAIEEDSIDTYLATFKEKYPDIKLNVLRLSVGGITARLLAEKDNPKADVLWGVAATS
                     LIILDDKEMLEPYAPKGFDKVNSAFKDDKKVPSWVGIDAWMTGITVNTKELKAKNISA
                     PSSYEDLIKPEYKDLISMPSPASSGTGYLTVSELIQIMGEEKAWQYMDKLHKNIGVYT
                     QSGSAPAILAASGEYPIGISFGNRGIKLKGEGYPVEVVFPKEGSGWEIESNALVKKDN
                     IKEASKVFLDWAISKDAMNEYSKNFAVTTIDTGNPVPEGFPKNPLEQMIDNDLKSAAR
                     NREAILNKWIEKYDGKTEKES"
     gene            26295..26825
                     /gene="rnmV"
                     /locus_tag="PC471_000135"
     CDS             26295..26825
                     /gene="rnmV"
                     /locus_tag="PC471_000135"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HDF2787283.1"
                     /translation="MIKEIIVVEGRDDVTAVKRAIDAELITTGGFGFPKGVMERIKAA
                     NERRGVIIFTDPDFAGEKIRKKIASEVPGCKHAFLPREEAKKDGDIGIENATPQSIIA
                     ALNKVRTENTEKRDEFKQVDLIRNGLIGNEEASLRRDALGKILGIGYGNAKQFLNRLN
                     NYGVEREEFIDALKQI"
     gene            26996..27865
                     /gene="rsmA"
                     /locus_tag="PC471_000136"
     CDS             26996..27865
                     /gene="rsmA"
                     /locus_tag="PC471_000136"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HDF2787284.1"
                     /translation="MDRLSSHNATKEVVQKYNFKFSKSLGQNFLIDSNIIDKILSGAR
                     ITRGDNIIEVGPGIGTLTREMGKIAEKVVAIEIDRNLIPILKDTLSDLDNTEVVNQDI
                     LKVDIQELVKDKLNGGPVKLVANLPYYITTPIVMKFLEEDIPVTDIVVMVQKEVADRM
                     NAIPGTKDYGALSIAVQYYCDTEIVAKAPRHMFIPQPNVDSTVIGLHVRDKRKYDVHN
                     EDIFFKTVKASFGQRRKTLLNSLGGLGFLNKDEIREVLKEANIDEKRRGETLSIEEFS
                     VLSNIINTKVSSK"
     gene            27931..29193
                     /locus_tag="PC471_000137"
     CDS             27931..29193
                     /locus_tag="PC471_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2787285.1"
                     /translation="MGSKRKFKRDYIILEAKDINFRYKERVLPKAFAKVEVNDEKAII
                     ALYVENLKYVKDGYKAVAIKDDHGLIDLGNIILNEQGRGEFVLDLEDYDDEIKGIALL
                     YERNIPLIGFKGNKLENYEDILFQEVEEEEYEEYEELEEEDEEIEENEESDRDEDSEE
                     EYIIEEYDEDEYEDYWEDDEDIEEGELKARTEVEDSDDEEIEEFNNYKEIENYNRVKV
                     QEEYIDIEYDEEEEEEEVFEVNREEDRFVSSKPTKSKKVQKDQNNIEKIKRANGVSQG
                     ESGNKQTSTLLMPRQIKKGLKLFKEVKPFVTDYIDKTRWWKIEINPTTLCGYTMPYLG
                     YLNVLNYTMYSDAVLHSYKYRHYLFGVQYDEYNKRKHYIYAIPGNRNEQPDNGSTGFL
                     RYQACDNRTNSLGYWLCFIDSRQRNIVR"
     gene            29344..30459
                     /locus_tag="PC471_000138"
     CDS             29344..30459
                     /locus_tag="PC471_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HDF2787286.1"
                     /translation="MINEQRILNEFLELVQIDSLSLKEGNVAKVLVKKLEEIGCSVVI
                     DNAGEKANGETGNIIATLKGNKEGKKILFSSHMDTVTPGIGVKPIVDEANGIIKSDGT
                     TILGSDDKAGIAAILEGLRYINENNIEHTDIQVVFSICEECGLVGAKNLDYGKIDSEY
                     AFILDSGGSPGEIIVKAPAQDVINVKILGKTAHAGLEPEAGISAIMVAARAIENMNLL
                     RIDEETTANIGIINGGTATNIVTGEVNIVAEARSLKENKLDVQTKHMVETFEKAAKDF
                     GAQIEIDVNRAYTPIDVSEDSEIIKLAKKAFSNLGIEGHTESTGGGSDTNILSKNGIE
                     AITLGIGMKNAHTLSEHIAIKDLYDSAKMVVEIIKEA"
     gene            30645..31541
                     /locus_tag="PC471_000139"
     CDS             30645..31541
                     /locus_tag="PC471_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HDF2787287.1"
                     /translation="METRYEEANKITIQSILWNIVLTIIKVIAGVIGNSSAMIADGLH
                     SASDIISSIGVLIGNYVSSRPGDREHNYGHEKAETLVSFVLSILLIFVSITIGIEAIK
                     SLFNLDALSVPSILPLVVSVISILIKEYQYRITIKVAKKINSPALKADAWHHRSDALS
                     SVAAFIGIGGSILGFKPLDPIASVVVAIFVAKVGISILISSVNELMDVSVDEEEIKEL
                     KFIVADTEGVKNLGDIKTRKHGAMAYVDLTICVDENLTVKQGHDIATKLEKHIIKHME
                     FVKGITVHVEPCTNCQGNKCNN"
     gene            31611..32408
                     /locus_tag="PC471_000140"
     CDS             31611..32408
                     /locus_tag="PC471_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2787288.1"
                     /translation="MSKKRKLKKKAAILIGVVVFFIIYFISFRIGINMEKDKSEVPKK
                     VEKEEEDNRSLRTQIKEKKKVYISDKNIENIKIEEDMWDEIKLFFSDFKKVRNTTSSY
                     EAVYEGYSDDGVKFSTDLNFFRVYTVNKEEYYKVPVSSKDAFKKLLDESIYTSFDLLS
                     HYKDWKEVTITHGKEIKKVHRWKFDDLAHKMISKRVVGKVQPEKSKERSDYNFTINIK
                     GDNFDVKVDTMGKDYVKVTSKKNVSYYEVHTSLFDYLKNEIFEIGKK"
     gene            complement(32463..33815)
                     /locus_tag="PC471_000141"
     CDS             complement(32463..33815)
                     /locus_tag="PC471_000141"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HDF2787289.1"
                     /translation="MNIHFIGIGGISMSALAEICINKGYQVSGSDSNESYLLDKLRDQ
                     GATIYIGQKKDNISDDVNMVVYTAAVHPDNEELVAAKEKNKLVMNRATFLGQIMREYK
                     NSIAVSGTHGKTSTTSMLSTIFEYADLDPTILVGGNLSMIGGNVKIGNSNHFITEACE
                     YVDSFLNFNPKISIVLNVEEDHLDYFSGIDEIKASFNKFGKLLPPEGYFIINGDDENI
                     DDILYDVKATIIKYGRDSDNDAVIKDIHFDNSGHGIFRIEYEGRDLGEFELSVYGLHN
                     IYNASSAIMAALVSGIDLETIRKNIKIYKGVGRRFETKGYYKNALVVDDYAHHPTELK
                     ATLAAAKKLKKSTLWCIFQPHTYTRTKSLLGEFSEAFYAADKVIITDIYAAREKDPGD
                     IHSKDLVEKLYQNNVDAIYIKEFEDIVKYLRENVKDNDLVITAGAGPIYKVADLLVEK
                     "
     gene            34034..34912
                     /gene="purR"
                     /locus_tag="PC471_000142"
     CDS             34034..34912
                     /gene="purR"
                     /locus_tag="PC471_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HDF2787290.1"
                     /translation="MKFKRTERIGAIVKILSDNPNKIYTLSYFTNQFNAAKSTISEDL
                     LVVKNVFEKLHLGKVITISGAAGGVKYIPKTSIAENAEFLMELCERICDKSRILSGGF
                     LYLIDLIYDPTIAAKIGKIFASNIEYVDADYVVTMETKGIPMALMTAKAMNLPLVIIR
                     KDIKVSEGPTLSMTYVSGNSSKVESMSLPRKALKPDSKVIIIDDFMRGGGTIKGMVDL
                     MNEFGAEVIGTGVFISTTNPSEKMVKDYISLIQLDVNGDKIVVEPNLKTFKDEYRNED
                     LDEEEDSEFEFEIDED"
     gene            35068..35346
                     /gene="spoVG"
                     /locus_tag="PC471_000143"
     CDS             35068..35346
                     /gene="spoVG"
                     /locus_tag="PC471_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation regulator SpoVG"
                     /protein_id="HDF2787291.1"
                     /translation="MKITDVRVRKLTEEGKMKCIVSITFDNLFVVHDIKVIEGHNGLF
                     IAMPSRKVGEGNFRDIAHPINAEMRQVLEDAVLQAYHEALVQWEVAAE"
     gene            35559..36938
                     /gene="glmU"
                     /locus_tag="PC471_000144"
     CDS             35559..36938
                     /gene="glmU"
                     /locus_tag="PC471_000144"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HDF2787292.1"
                     /translation="MNFKAIILAAGKGTRMKSKYPKVIHKVCGKEMVNHIIDVSKKSG
                     VKDTVVILGHEADVVKEKLAEEIIIAMQTEQLGTGHAVKMAKEYINDEDTIVVLCGDT
                     PLIKEETLKRLFEYHIENKYHATVLTTRVGNPTGYGRIIRDKKGDLLKIVEQKDANSE
                     EKMISEINSGIYCFNGKSLREALDLLNNNNSQGEYYLTDTAKIMRDKGLKVGAFAGST
                     IEELMGVNSRVELSKAEEIMRRRINESHMVNGVTIIDTNSTYIESDVMIGNDTIIYPG
                     VMLQGKTRIGSDCIIGMNSSITNSEIGDGTEIKNSTIIDSKVGENSTVGPYAYLRPKS
                     DLGNNVKIGDFVEVKNAIIEDGSKASHLSYIGDAHVGKNVNIGCGVVFVNYDGKNKFK
                     SIVKDNAFIGSNSNLVAPVVVEEKGYIATGSTITHDVPDGALAIARERQVIKEGWVEK
                     KNQKDDQSK"
     gene            37033..37983
                     /locus_tag="PC471_000145"
     CDS             37033..37983
                     /locus_tag="PC471_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003361732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HDF2787293.1"
                     /translation="MNTSGSEIKIIAGNSSKELAQKIADYIGVSVLDCEVGTFSDGEI
                     CVNMNETVRGCDVFVVQSTNSPVNDNLMELLILIDALKRASAGRITAVIPYYGYARQD
                     RKAKARDPITAKLVANLITAAGADRVLTMDLHAAQIQGYFDIPLDHLLGGTILANYFN
                     EKKIEDLVVVSPDLGSVTRSRKFANTLNGEVPIAIIDKRRPKANVCEVMNLIGDVKGK
                     NVILLDDMIDTAGTIVNAANALKEFGAKDVYACCTHGVLSGPAIERIANSEISELIVL
                     DTIQLPEEKRIDKIKIKTVAPLFGDAIRMIFSNESVSKLF"
     regulatory      38821..38906
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01786"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01786"
     gene            39068..39589
                     /locus_tag="PC471_000146"
     CDS             39068..39589
                     /locus_tag="PC471_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HDF2787294.1"
                     /translation="MKLKKNKKGFTLVELLVVIAIIGILAVVAVPALFSNINKAKVAS
                     VESDYSSVKSAALSYYSDTNKIPVTPDGQTGLSVLETYMESLPDKADIGGKYKLIKVG
                     NKLVLQIGTNTEGVTLTEAQSAKLLSDIGENKIYTSATTNSLGNPLTSNTKIDNNVLY
                     IVLIDNTVMDTTK"
     gene            39740..41416
                     /locus_tag="PC471_000147"
     CDS             39740..41416
                     /locus_tag="PC471_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II/IV secretion system protein"
                     /protein_id="HDF2787295.1"
                     /translation="MAKKVRIGDKLVEKGYITEEQLKWALSEQKNSGKRLGEFLVQEG
                     LIDSNLLISVLKELLDIESIFLEGTEIDTLATKMVPENICKRYTVFPFKIDGNKICLA
                     MSDPQDREAVQDVRRMSGKDVEIFISSTEDINKAIGHAYAHSEINKAMTEYNKNRTGG
                     VRETVILEEDVNAAPIVRLVNNILENAVRMEASDIHIEQSENYMRVRFRIDGMLREYM
                     RMNSAPYKAVISRIKIMSDINISEKRIPQDGRIYLKVDNKPIDFRVSTMPTNRDEKIA
                     MRVLDKSNFMVSKEVLGIDEHGSKIYDELINTPYGLILVVGPTGSGKTTTLYSMLNQL
                     NTENRNLLTIEDPIEYELPGVNQSQINEKAGLTFASGLRAFMRQDPDIIMVGEIRDTE
                     TAEIAIRASLTGHLVLSTLHANTAVGAISRLLDMDVESFLITSSVLGVISQRLTRKIC
                     EHCKVSYEADIGEKKALGIDVNESVTIYRGKGCERCNNTGYKGRLGIFEMLEITPEIK
                     ELIDSSANQREILKMARKQGMVSLKEDIVKKVLNGKTTVEEMIRIILMTD"
     gene            41428..42636
                     /locus_tag="PC471_000148"
     CDS             41428..42636
                     /locus_tag="PC471_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HDF2787296.1"
                     /translation="MKSFKYTVIKEDGKKQTDVIEANDFNEARNLLRKRNLRVIEIKE
                     SKNKNIRLSSRKRKKDLGADQISHFCRQFAIIVSSGINSISGLETLARRSTNITLREE
                     INRIVAEIKVGSTIADSMLSSKSKFPKLLGAMVATGEATGKLDEVLKSMASFYASEHR
                     VKQKLRNAATYPLIVLISSFIMIFIFTTFMVPKMINSITTVGATLPLITRIVMGFGMF
                     MKKFWIIVLLVIILFIYQFKKYLKTSVGRAHKDRVINKIPVLGKGINCMVATRFSRAL
                     YLFVSTGYPLVQGLDYIIDSVNNTMAEKILASAKDGITRGEGLAENLERYTYFDSVLV
                     QMIAIGEQTGELENISRQMAEFYEYESEIYLNRMASMIEPVLIIAVGIIVAILVVSIF
                     MPMLSIYDAM"
     gene            42651..44363
                     /locus_tag="PC471_000149"
     CDS             42651..44363
                     /locus_tag="PC471_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HDF2787297.1"
                     /translation="MNKVYVSYYGDSISIVEGRYKHDKDKFYVKNFNVFSIEDIAQDF
                     SKEKYALLRYALENVKLKSRHVVFCLNTRDVILKPQKLPNMDKKDLDGFMKLEMDEMM
                     ALESDEYVFSYEVSSETPDNSDGDESSLNLIIGAIQKEEVNAIVEIMHEFDLVLDRID
                     TLSTAYLRMLKNLDYNDIMVTNISDNSTIINIYKKDTLFISDNIPIKITAKDMDMQLL
                     NVVEESKGLMNYYSSRNFGKVTDTILILGKRYSNKLIYETFSSAFSSYVSQGLTEIMD
                     ISDMITGDIDEDDINSIVELLGSMIEPKGKRDFENINFLPMDILRKQIKAKQMKNILK
                     IAPIVILILAIPYLCLIFSNNLANKELENVNGEIRNIESAYYQIGNIEKMIQSKSEEI
                     KVYDMLIGKKVKWENLLDDIDKNTPSKVELTSLSTTYQNLGTNNKADNSKSNKTTTDK
                     SSNNETKSSTKSDSNGDKTDNDLNEDKTTNVSEALTGNSETNSNNTDKDDKTPLYDKI
                     PNVINIEGNAKDSSYVGQFLYKLKGLYYFKDVKLHSIKSNAEDSNYTFSMTLYLKEGV
                     LTNE"
     gene            44356..45264
                     /gene="pilO"
                     /locus_tag="PC471_000150"
     CDS             44356..45264
                     /gene="pilO"
                     /locus_tag="PC471_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HDF2787298.1"
                     /translation="MNKDIFKKDIKDIFKMEVSLKGILIKVIILLAILSYGYFGLYPQ
                     YQKYIATKNSVKQATEKLNIYKEKTALMPKYEKQLEEVESELKEKSKNLLYDMQDGLF
                     LIGLDKKMKAYNIDLTSYDVGNVKEYEHFYGISTTISVRGDYRNVKKLMAYLEEQKNV
                     TQILDYTMTSYIEPEKEEEETTNNKDLIKNVNINKDTKIYWVEGENKWHLYKDCPLIK
                     EGEILKNDKLLDRSTNYLCNTCVLRAATEKEAKKEVVAPKAEGVVDATFKFIMYTKEN
                     TTMILDTDNPSSWRPGKYNPFKSSAK"
     gene            45291..45857
                     /locus_tag="PC471_000151"
     CDS             45291..45857
                     /locus_tag="PC471_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HDF2787299.1"
                     /translation="MSLYKNNEKGLTLLEVIIAVFILTIVLSISYKVFNGITSAVKKQ
                     QIITDAQVNINLINKYLNRDLENCKELTKTGSGNNYEYNIEMPDNVVKYEVSIETKKN
                     TEVYSVTRIQNNTIDTENEVREEIIYNQPLVQNNKEMKETPFKIEKQTGKSIYTVSIY
                     YNESVQESHKNSNLNNKTYTFDVMSRIG"
     gene            45894..46421
                     /locus_tag="PC471_000152"
     CDS             45894..46421
                     /locus_tag="PC471_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HDF2787300.1"
                     /translation="MSKKNSERGFSLIEVLVAMAIMGIVLFAFFNIINTNNKANTKND
                     TDITSLNYVQSEIENLREKIKSGEFDFDSLDKLEDGTVVYEKLIDKSKKVVYDKVLSE
                     GDVSLYDTPYEKITTIKDEDGNLIDKGNITNKIKTIVEDKSGQIYKIAVTGKSMNDYS
                     SKKEVKIVTEIFKDK"
     gene            46442..47980
                     /locus_tag="PC471_000153"
     CDS             46442..47980
                     /locus_tag="PC471_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2787301.1"
                     /translation="MRKWNKFKSERGAALVLVLVVVALLSIVGLIFSNQIANRIKSTK
                     TTNEGIQAKYLAETCVENSIDKAYEKLYDELEKMDNEFKSENQEKSISRSKLRDISDE
                     DFNNQDEENIEAERLGYMNNINFYLNKASSDLEKASMELKKLYDLDMLDYRDIEYVDA
                     NIISHRDSILEICKNYTSGDISKINEYILKEDIDSTTLIEAKLVNNDILLKMFLEENK
                     IENEHLNSAFSHTYKALDNISLAMQNMIEYRHTFHIDEPKVEVSNGIPDSQQYYELIQ
                     NPIINSMEYIWNSKWDTLEKLLEILPNQTQGFNSLRVHLRNNVRKFEKLSDNISSGKK
                     NTAKNFLKYKELLYEISDQCNQLKSMSYEKIPVKYDNMALITTFDYIQNELLAEIKCR
                     LKELKPQEIDKTEGITIKIPFYKADYDMTKEGWPKLKENGSGAELSLMVTGDKDGIKE
                     VEVTDGKKNIIGLGVEENSNSKYKVDAIVNFNLNIDTNVVGNYDIKDKILINHDISSY
                     KKVN"
     gene            48080..49162
                     /locus_tag="PC471_000154"
     CDS             48080..49162
                     /locus_tag="PC471_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HDF2787302.1"
                     /translation="MYIYDLLEQGIRLNASDIHITVGTNPVARVKGGFVKLSEQILTS
                     EVTMQMAKDIAGESMFKVIEEHGEADFSASLKTGERFRVNAYRQKGNYAIAIRTITAE
                     IPTFEKLGLPESIKSFTEKHKGLVLVTGPTGSGKSTTLASMINIINEKQQKHIITLED
                     PIEYVHHHKQSLVNQREVGTDTESFHSALRAILRQDPDVILIGEMRDPETVSIALTAA
                     ETGHLVFSTLHTVGAAKTIDRIVDMFQPSQQQQIKTQLSTVCEGVVSQQLLPTADGKG
                     RIAAIELMFATPAIKNLIREGKTYQIPNMIQTGVKVGMKTMDQDLMELYKNGKITKDM
                     ALSRCTDQEFMTRMIGGVNYNGYYNR"
     gene            49143..49925
                     /locus_tag="PC471_000155"
     CDS             49143..49925
                     /locus_tag="PC471_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HDF2787303.1"
                     /translation="MDIIIVNLYVFMVGIVFGSFFNVCIFRIPEGISISRPPSHCMSC
                     NTRLKPIDLIPVLSYLMSGRKCRYCYEKISSRYAIVELITGLLFLTVFMLYGISTSTI
                     YYLVLVSLLIIITFIDIDHFIIPDKILVFGAIFSMIFNLLFKEIPVKDSILGVFICGG
                     SVWIIVLLIEFVIKKECMGGGDIKLFAMLGFYMGVKNGLLTALLSVYVGAVYGICVII
                     ISRIKGKEYNSVIPYGPFISIGALITILCGKQLLELYFSMVI"
     gene            50027..50677
                     /locus_tag="PC471_000156"
     CDS             50027..50677
                     /locus_tag="PC471_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HDF2787304.1"
                     /translation="MEYIIIISLFFAHLINKSLCVFCDREKKNLYNTFFIYIITVVLS
                     VLIYLKFGISVECVQYMVLIPFLIIISIVDYYTIYVYDITIVSGIIVQGIIFLVFFNI
                     VQFDYINHIFGLLIGFIIPYIIAKLTKGLGYGDVGLYSLCCFALGNNYGFYVIVLSFI
                     LAFLFFIILYITKIFKDVDIRKQIPFTPFISLSTILIIFTEYNIFNFYYNLLYKFI"
     gene            50691..51251
                     /locus_tag="PC471_000157"
     CDS             50691..51251
                     /locus_tag="PC471_000157"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HDF2787305.1"
                     /translation="MYVVVGLGNPGKKYEKTRHNVGFDVIDILAKEYNISVTKIKHKA
                     LIGEGRVGTEKVLLVKPQTYMNLSGETLIDIYKYYKVDLSNIVVVYDDIDLEVGKIRI
                     RKKGSGGTHNGMKSITKCLGSNDFPRVRVGVSKPEAGQDLADFVLSRFRKEESDNINE
                     ALEKAADAIDSIIRENIDMSMNKYNG"
     gene            51268..54654
                     /gene="mfd"
                     /locus_tag="PC471_000158"
     CDS             51268..54654
                     /gene="mfd"
                     /locus_tag="PC471_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HDF2787306.1"
                     /translation="MNDVFLYPLQNSKEYKDIINCIKNTKGSLLVNGLLPVQKPHISY
                     SIFNDLSRQMIFITSSDLEAKKVYEDLSFYMEDKVEYLGFQDIYFYHLDAKDRNEEAK
                     KLKVLLKLANKEKIILVTSIEAVLRKYIPKQVLLDSVSHYKVGDSLDLEKLTEKLVSL
                     GYERVSKIEGFGQFSIRGGIIDVFSLEYTNPIRMELFDDEIDSIRTFDVYSQKSIDKL
                     QQFSITPSREFIYPEKTTDALVKLKKETTKNTDEGTFQNIDYISSKTYFEGVENYIDY
                     IYPEENKSIFTYLADDAIVFINDITRLKERCENYINEFRENYKLNLERGLAIKNQGKL
                     LYHYTDLEYLVKDKSVVLNSLLPKSINNFSIKSIINFESREVPTFNGKVDLLVEELNR
                     LKYNGYKIILATNTLERANKLGKDLLDKGLETTISKDRDIEIKSSQVIIVPAHINSGF
                     QYKSIKFVVITDNEMIGVYKRASKTSNKKVKKGKKIESFLDLSVGDYVVHENSGVGRY
                     TGIEQITVNAIKKDYMKIVYQGGDNLYVPIDQMDKVQKYIGAEVEKVKLNKLGTNEWT
                     KAKAKVKKEIEDMTKDLIELYAKREKIQGYKFSKDTPWQAEFESLFPYQETEDQLKAI
                     EETKKDMESSKVMDRLVCGDVGYGKTEVAIRSIFKACMDQKQVAVLVPTTILAQQHYN
                     TFKERFENYPLRVEVLSRFKTPKQQKQIIEDAKKGLVDILIGTHRIISKDINLPNLGL
                     VVIDEEQRFGVKHKEALKKIKSTVDVLTLSATPIPRTLHMSLSGIRDMSVIEEPPQER
                     HPVITYVTESKESVIQDEIERELSRGGQVFFVYNRVEHIEEMASMIQKLVPDARVAVA
                     HGRMTSKSLENIILGFLNKDYDVLVCTTIIETGMDISNANTMIIYDADKMGLAQLYQL
                     RGRVGRSSRQGYAYLLYEKDKTLSEIAEKRLKAIREFTEFGSGFKIAMRDLEIRGAGN
                     ILGSQQHGHMAVIGYDLYVKMLNDAIKKVKGEPIVEEIDVEIDLSVNAYIPDNYIKDE
                     LIKIEMYKKIASIENKEDMLDIQEELEDRFSDIPKPVQTLLTIAYIKSLCKILKIEKI
                     RQLKDEILLVPITKYRTKQKIGYNIVTELEELLEKMCKVK"
     gene            54685..55680
                     /locus_tag="PC471_000159"
     CDS             54685..55680
                     /locus_tag="PC471_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HDF2787307.1"
                     /translation="MKKVITLVIAMILVVSVTACSSSKGETVATVEGTKISSDEFKKT
                     IALYKDSMEQTYGKDIWDKEVEKGVKYKDKFKDLILDQLITTEVIYSQAKKDNLLPKK
                     EDVEKSFKELKDAMGKDEKYKEQLKKLGIDDEFLRDQQEKDLAMQNYQSNFAKKTKIS
                     DEEMKKYYDTHKDEFKKDEVEASHILLKTVDDNNKPLSDKEKAEAKKKAEEALKEVKS
                     GEDFAKVAKKYSQDTSASDGGKLGFFSRGQMVAEFEDAAFSMKKGEVSDLVETQYGYH
                     IIKVTDRINEQTSFEDAKETIKDQLLKNKYQEQIEKLTKEAKVEKDEKVINKITI"
     gene            55804..56343
                     /gene="spoVT"
                     /locus_tag="PC471_000160"
     CDS             55804..56343
                     /gene="spoVT"
                     /locus_tag="PC471_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein T"
                     /protein_id="HDF2787308.1"
                     /translation="MKATGIVRRIDDLGRVVIPKEIRKTLRIREGDPLEIFTAKDGEV
                     ILKKYSPIGELNEFSQEYAETLAEVLGYGVLITDLDSIIAVSKLPKKDYKEKSISDEL
                     EAIISERTVKYSKDKNILPLFKEDQKEYTSQVIMPIISASGDCIGSIVVVSKDKDTIA
                     ESEEKSLKIAANFLGKQVQ"
     gene            56463..58076
                     /locus_tag="PC471_000161"
     CDS             56463..58076
                     /locus_tag="PC471_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HDF2787309.1"
                     /translation="MNNNKNKDSFLKGALILGLAGVIVKIMGGFFRIPLGNMIGSEGM
                     GYYQAAYPVYTLFLTLATAGFPTAVAKLVSEKVAIGNFKGANKIFKVSHTVLFITGII
                     SFCILFFGADYIVTNVMKNPGALYSMKAIAPALLFVPAMSAYRGYFQGRQDMTKIAVS
                     QVAEQFFRVVLGLTLAYLLMKSLGQTFGAAGAISGATIGSIASMLYLVFAYMLGRKER
                     RAEIDASQRFKDERVSYIFKKLLTVAIPITIGASVMPLVNMIDNVIVIRRLMEAGFTY
                     KVANSMFGQLTGMAMATINLPAVITTAMSMSLVPAISKAYALGNKSKARKDTKSAVKV
                     TLLIVLPCAFGMASLAIPIMGLLFPHEPSSVGTILFTLTPCVLFLGLIQTLTGIIQGM
                     GKPIVPVIALCVGMLCKIVISYTLTGIPDINVLGSAFGTVTAYFVAAMINLLYVKKHM
                     NVNFSKKEFIIKPFITVMTMFIMVKLSYGALVGFLGNSISTIIAICVGGIVYVVVILG
                     IGGIKKEEILTMPKGDKLYKLLKKVKLIR"
     gene            58183..59631
                     /gene="mazG"
                     /locus_tag="PC471_000162"
     CDS             58183..59631
                     /gene="mazG"
                     /locus_tag="PC471_000162"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HDF2787310.1"
                     /translation="MGKISIVGLGPGDYSLISQGVLESLINSPKVFFRTEKHPIIDKL
                     KETIQYTSLDYFYEKEENFDNVYLKISEFIIEESAKGDLVYAVPGHPRVAEKTVSIIE
                     RLANEKHIAVEILPSMSFVDAMFNYLAIDPSEGFKLVDAFEIENSYIDLDTSMIITQV
                     YDKFIASNVKLKLMEHYDYNQEVCIVNGAGVKDLESKKFIKLCELDRNENFFDYLTSL
                     YIPKSSKKMYNTVHDLEIIVNTLRSPSGCEWDKKQTHQSLKNSVIEEAYELCNAIDNN
                     DIDEMVEELGDVLLQVIFHCQIGNEEGYFDLKEVVNGICKKLIHRHPHIFSGAKLDMS
                     KFEKTWEELKKEEKGESTITDGLRRIPKHLPALMKASKVQHKAALVGFDWDNVEDVFK
                     KIEEEYQELLDEHRVGNIKYIKEELGDLLFSIVNLARFLDIDSGEALNCTTEKFINRF
                     DFIEKSAMNLNKKLEDMTLEEMDEFWNQAKKQ"
     gene            59720..59998
                     /locus_tag="PC471_000163"
     CDS             59720..59998
                     /locus_tag="PC471_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003153447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HDF2787311.1"
                     /translation="MNKAELVSKMAEKSGLTKKEAEAALNAFMSSVQDALVNNEKVQL
                     VGFGTFETRERAARQGRNPRDPEQVIDIPASKAPVFKAGKGLKDIING"
     gene            60070..60312
                     /locus_tag="PC471_000164"
     CDS             60070..60312
                     /locus_tag="PC471_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HDF2787312.1"
                     /translation="MRLDKFLKVSRIIKRRTVAKDACDKGIVTINGKVAKSSSEVNIG
                     DTLEIQFGEKKMKFKINEVKEHVLKNDAKEMYEILE"
     gene            60374..60628
                     /locus_tag="PC471_000165"
     CDS             60374..60628
                     /locus_tag="PC471_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein YabP"
                     /protein_id="HDF2787313.1"
                     /translation="MEQNITLKDRSKLVISGVEHIYSFNDKRVELKTSVGEMVIEGEN
                     LDMSKLSIDENIISIDGTINSMVYAKPKKPQESFLKKVFR"
     gene            60629..61162
                     /locus_tag="PC471_000166"
     CDS             60629..61162
                     /locus_tag="PC471_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2787314.1"
                     /translation="MIPFTQDVSIFYATIYGGILIGVLFDFYRGLRGNFKFINYFAII
                     FDVLFWFLATVIIFVTINLTEFFDLRYYHFVALFIGFILYYNTISKIVLSIINKIIRF
                     VRNSFKKVTHYIVSFLNNLYYVIIYSLHLLFDIIFYIPNIFIATRKSIKRKSNKKLKN
                     KKKSKPKKKKNKTKKRV"
     gene            61168..61452
                     /locus_tag="PC471_000167"
     CDS             61168..61452
                     /locus_tag="PC471_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HDF2787315.1"
                     /translation="MNLRKKFSGQIIVISLFLGISIFSMMTGFVFEYTKAKEYKKEIA
                     SLNKQLKKTEIQINSLKKDEKSYEGDLEDIARKRLNMVKPNETVYVDINR"
     gene            61565..62485
                     /locus_tag="PC471_000168"
     CDS             61565..62485
                     /locus_tag="PC471_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA family phosphatase"
                     /protein_id="HDF2787316.1"
                     /translation="MKVGAIDIGTNSMRLLIADYRDGNFINRKKFVNTTRIGYGVDSK
                     GYIKEDAIKRNIKALKEFSDKCNDEKCEKIYCMGTSALRDSKNGDYFVKLAKEETGID
                     VDIVTGIEESNLGFKGVLEGIQGDDTIIVIDIGGGSTEFIVGDSEGIIFNKSENVGAL
                     RMTERFLKHDIIEDEEFEAMSSFIYGEIDKTLNHIKDKGLKTLIGIGGTITSLSAMNQ
                     ELEIYSMEKIHNSKIHKKEIEEILQKLIKMTLSDKKNLKGLQEKRADIITAGVKILNI
                     IMEKLEIEEITVSEYDNLEGLICQKSKKMY"
     gene            62575..64947
                     /gene="spoIIE"
                     /locus_tag="PC471_000169"
     CDS             62575..64947
                     /gene="spoIIE"
                     /locus_tag="PC471_000169"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein E"
                     /protein_id="HDF2787317.1"
                     /translation="MQRSVAVVKNKTVKPNVIKLISGYIDFTTAAFAVMGFLLSRSII
                     VDSVAPLGIAFFICASKIDKYKIPVFISTLLGILLSSNDFGNVAKYAVCLTIFMAMNK
                     KLKLSDSIAKVAFIGAIILLPISMGQAFFSNRYVYGLLIAGVEAIIMFISIYVFSYGI
                     GLMANVNNRMSVRTEEAISISLLLTFSIMGIGDISLFGISIRTILSTVLILLTSIVGG
                     ATMGASSGVIIGVASILNNMTSAVYMGIYSFSGLISGAFNKINKYFCILGYILSWTII
                     YLYTSGITSNMMQLRDILLGCLIVLVLPERLFNKIEKLIKSNVASNEIVYDYIMRSKN
                     LTNSRLNSIYKTYDDLADTFDKIREKDKVLDQRDIANVIDMIHNDECKSCSMRRMCWE
                     SRFNHTYTMIYNILEKIEEKGELSLNDIPKNFRKECMKPESIVKISNHYYKMFVLDYD
                     WSVKFSESRKLIANQIRSISKSIKSLSQDLEGDIMLDIEKEKNIYEQLERYDITVDKV
                     SYLTKSNSEFEISIEKKTCHDGCMCEDKIVNIISDLVGENMSVRKIGCHCLGGKCKAT
                     FVKSQKYKAVTEVSAMSRDGHILCGDNYTYMEINDGKYMMAISDGMGKGKKAYEESSA
                     TIDILEKMIDAKIKDEIVIDTINNMLLLKSSEEMFSTLDLGILDLKRGCLETIKMGAC
                     STYIKREDGEVDLISSSSLPVGILSDVKIDRKNVKVKEGDYVIMVSDGIVDAGRNNNL
                     GDNWLIYFLKNIETTNPKEISNLILDRALELQALQIEDDMTVLVTKICTK"
     gene            65298..67190
                     /gene="pepF"
                     /locus_tag="PC471_000170"
     CDS             65298..67190
                     /gene="pepF"
                     /locus_tag="PC471_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HDF2787318.1"
                     /translation="MNRKLCKAIIPLFISIFCMLIISIKADQGYQKNNDRAITVMNNN
                     DTEDDAEKKQEIDYEKTCWNLQSLFKSDDQWKKELKSFDKDTKELKNYIGKVTKSPTH
                     LSFALNIKEKLDIRLNKLSAYVKLKQDTNKNSYKYLDMNDSMSKSYGNYMGICSDLEL
                     EILKLSNKDYKKIISNKNINRKYGAYLRDIRRNKVHYLDDKSEDILSKVSSISSLPSQ
                     VYELFRNMDRKTNLTPAQYASELESSDRENRKKAYQDELITYNDNINTIAGLITGQVK
                     KNIFYSQERGYESSLEMYLESDNVDEKVYNNLIRTVNSNMDSLHKYIDLRKKVLKLDK
                     VHSYDMFVPMVKAVDNNINYEKAQSIVYSALSPLGKEYNDVVYKAFNEKWIDVYSHEN
                     KVSGGYCLSVYENHPYILLNYNNSLGSVSTLSHELGHAVYEYLSSKNQNYFNSNPSIF
                     THEVASTTNEALLYEMLIKEAKGNDEKAYYITQYLDLIKDTLYTQTMYAEFEKTIHEL
                     VEKGKSVNALVLNDVWGQLLKKYYGQSYELDQLSKVGWARIPHFYNSFYVYKYATGCS
                     AGVSFAQDILKNGSDNYMSFLKKGSSDYPINLLKSSGINLTNTKPIENTIKKFDSLVA
                     ELEKLL"
     gene            67304..68269
                     /gene="galU"
                     /locus_tag="PC471_000171"
     CDS             67304..68269
                     /gene="galU"
                     /locus_tag="PC471_000171"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HDF2787319.1"
                     /translation="MQVKVKKAVIPAAGLGTRFLPATKAQPKEMLPIVDKPTLQYIIE
                     EAVASGIEEILIITGRNKKSIEDHFDKSVELELDLEKKGKKELLEVVQNISNMINIHY
                     IRQKEPKGLGDAIYCARHFIGDEPFAVMLGDDIVDNDVPCLKQLTDAYEEYRTTILGV
                     QKVNQEDTNKYGIIEAKNIEGRVYKVKDMVEKPESGKAPSNIAILGRYIITPEIFDIL
                     KDLPPGKGGEVQLTDALKILSKKEAMYAYNFEGKRYDVGDKLGFLEATVDFALKKEDL
                     KEDFIKYLKHVCSEFDKNNNVLYNNTDEKIDLIEVNEARELEIQK"
     gene            68769..70373
                     /gene="rho"
                     /locus_tag="PC471_000172"
     CDS             68769..70373
                     /gene="rho"
                     /locus_tag="PC471_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HDF2787320.1"
                     /translation="MRRIFLILDDKVLEGLGSKTLVELREIAKELKIKSITTYKKNEL
                     IEIINSKSKNEEKVNEETKEKNEDIKEDIKNRKENIERESIEKEVEYKSPTKSYNKKE
                     IDTQNISQNQEHRNQRNDYNKTTSVNDSNKSSNISNRMVRNNNKNYYMPKQVDESKIV
                     DEFNTSKEDEVVGVLEILPDGFGFLRGSNYLSTEGDVYVSPSQIRRFNMKTGDKIKGI
                     TRHPKSGEKFRALLYVQKINDENPDTAIQRNAFETLTPIFPEERLTLETNRNEIATRI
                     IDLISPIGKGQRGLIVAPPKAGKTVLLKSVANSIAKNHPNVELIVLLIDERPEEVTDM
                     KESIEGDVIYSTFDQVSSHHVKVAEMVLNRAQRLVEHGKDVVILLDSITRLARAYNLT
                     ISPTGRTLSGGIDPGALHGPKKFFGAARNIRQGGSLTILGTALVETGSRMDDVIFEEF
                     KGTGNMELHLDRKLAEKRIFPAIDIYKSGTRRDDLLLDDEEKTALWRLRREMSNNSVM
                     EITDKVIELIKRTKDNKEFVKSIKNL"
     gene            70526..70726
                     /gene="rpmE"
                     /locus_tag="PC471_000173"
     CDS             70526..70726
                     /gene="rpmE"
                     /locus_tag="PC471_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018591942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HDF2787321.1"
                     /translation="MQKEIQPKYNPVEVRCACGNTFVAGSTKDEIKVEICSECHPFYT
                     GKQKNIEKGGRIDKFKKRFKMD"
     gene            70871..71764
                     /locus_tag="PC471_000174"
     CDS             70871..71764
                     /locus_tag="PC471_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1385 domain-containing protein"
                     /protein_id="HDF2787322.1"
                     /translation="MAKQAVGGQAVIEGVMMQSKDKRAVAVRKSDGEIALKEDRIKSW
                     VRDKNIDKIPFVRGSFVMIDTMIQGIKSLNFSSEFFMEEAEEDKFDLFIKKIFKDKAN
                     DIIIIFSLVIAMLLSAGLFIFIPTLVGGAFSKVMPNDFMLNLIEGIIRIAILFAYIVL
                     ISRSKDIERVFQYHGAEHKSIYCYENDLELTVENARKFKRLHPRCGTNFLFIVMAVSI
                     ILFAFFGWPNPILRIFMRIICVPIVAGLSYEVIRVLGKYDNGFTKIIAYPGMMLQYFT
                     TKEPDDEQLEVALEALKAVVD"
     gene            71782..72630
                     /gene="prmC"
                     /locus_tag="PC471_000175"
     CDS             71782..72630
                     /gene="prmC"
                     /locus_tag="PC471_000175"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HDF2787323.1"
                     /translation="MTIKDIIIKYSDKLKDISDTPRLDTELLLQKTLGVDRLYIHLNL
                     NKELTEEQKTKFMGFAEERLNGRPIAYIVENREFMGLDFFVKEGVLIPRPDTETLVEE
                     IIEICREKKDVSILDIGTGSGAITISLAKYIENSKIMSFDISETALEIAKKNAIINEV
                     GEKIKYINSDLFTAISDSNIKFDIIVSNPPYIKKQDIETLHKQVKDYEPYNALEGGED
                     GLDFYRRITEQGKKYLNKCGILAYEVGHNQAEDVINIMKSNGYKKIYTKKDIQGIDRV
                     VIGYNI"
     gene            72674..73738
                     /gene="prfA"
                     /locus_tag="PC471_000176"
     CDS             72674..73738
                     /gene="prfA"
                     /locus_tag="PC471_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HDF2787324.1"
                     /translation="MLKKLEVLEDTYKDLSEKIGDPDVINDQKVWQKYIKEHADLEPI
                     VMKYREYKSVLDSIKESKEILQEESDEELRELAKMELAEMEEKVAPLEEEIKILLLPK
                     DPNDDKNVIVEIRGGAGGDEAALFAGDLFRMYSRYAERRRWKIELLSASDTGVGGYKE
                     VSFMIKGKGAYSRLKYESGVHRVQRIPSTESGGRIHTSTSTVAVLPEVEDVEVEINPN
                     DLRIDVFRSSGNGGQSVNTTDSAVRVTHIPTGEVVSCQDGKSQLKNKEQALKILKARL
                     YDKALAEQHKDIAAERKSQVGTGDRSERIRTYNFPQGRISDHRINLTLYKLDAFLDGD
                     IDEMIDALITVDQTEKMTAI"
     gene            73867..74547
                     /locus_tag="PC471_000177"
     CDS             73867..74547
                     /locus_tag="PC471_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HDF2787325.1"
                     /translation="MILKVTIIGLLAGVIGTGLGGVISAIFKREVDKYLSFFMGLSGG
                     IMLAVVVFDLMKESMDKMGIINTVIFTFVGALITMYIKTKLDVSGNMASGYLIFISIL
                     LHNLPEGLAIGSSFMSTESLGITLAIVIGLHNIPEGLAMALGLVCNKMKLSKVILFTV
                     IAGLPMGLGSFLGVYFGGVFTSLIGVFLATAGGTMMYVVLEEIFPHSKSVYCIIGFLL
                     GTMIVNYI"
     gene            74576..75616
                     /locus_tag="PC471_000178"
     CDS             74576..75616
                     /locus_tag="PC471_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HDF2787326.1"
                     /translation="MKTIISNIDINNIDYEEIKIQAKLLREGKTVIFPTETVYGLGAN
                     ALDENAVKKIYEAKGRPSDNPLIVHIYEKEEVYDLAKDISDKAKLVIEKLWPGPITII
                     LNKKDIIPYKTSGGLDTVAIRMPSNVIARAIIKEAGIPIAAPSANISGRPSPTKAKHV
                     YEEMNGRVDGIVLGGDSNFGLESTVLDLTEETPMILRPGSITKEVLEELLGEVKLDPS
                     LSKKEDNQKAKAPGMKYKHYSPNADVYIISGKRENVATKINDMIKSNRDKGLKTGVMC
                     ISRNREFYDGEVIDLGNSLEEVASNLFDALIEMDKKGVDVIYSEEFPKTGVGQAIMNR
                     LLKSAGYKVIKA"
     gene            75633..76082
                     /locus_tag="PC471_000179"
     CDS             75633..76082
                     /locus_tag="PC471_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight protein arginine
                     phosphatase"
                     /protein_id="HDF2787327.1"
                     /translation="MNILIVCTGNTCRSPMAEAILRKAIKESGRSIEEYSISSAGIST
                     ANGMGASENSIEVLKEIGIDLSNHRSKVITKKLIDESDIILTMTKSHKEILVQAVPKC
                     KEKVYTFKGFANKNEEDISDPFGGNLDIYRSTMREIMYSVNEIVKKI"
     gene            76099..76551
                     /gene="rpiB"
                     /locus_tag="PC471_000180"
     CDS             76099..76551
                     /gene="rpiB"
                     /locus_tag="PC471_000180"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HDF2787328.1"
                     /translation="MKIGLGCDHGGYNLKKEIISYLESKGIECVDYGTNNATDSVDYP
                     VYGEIVANSVINKEVDYGILCCGTGIGISLAANKVPGIRCAVVSDVFSAKMSKAHNDA
                     NMLSLGERVLGKGLALEIVEAWINTDFEGDRHARRVNMIKSIEEKHNK"
     gene            76587..77216
                     /gene="upp"
                     /locus_tag="PC471_000181"
     CDS             76587..77216
                     /gene="upp"
                     /locus_tag="PC471_000181"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HDF2787329.1"
                     /translation="MSKVVETNHPLIQHKLTLMRDKNTGSKDFRELLTEIAMLMGYEI
                     TKDIPLKDVEIETPIQKTSSKVVAGKKLAIIPILRAGLGMVDGLVSLMPAAKVGHVGL
                     YRDPETLKPVEYYCKLPQDIGERDIIVVDPMLATGGSAVAAIDLLKSKGAKSIKLANL
                     VAAPEGIAEVQKYHDDVDIYVASVDERLNEHGYIIPGLGDAGDRLFGTK"
     gene            77511..78326
                     /locus_tag="PC471_000182"
     CDS             77511..78326
                     /locus_tag="PC471_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF2563 family selenium-dependent molybdenum
                     hydroxylase system protein"
                     /protein_id="HDF2787330.1"
                     /translation="MKNCIVVRGAGDLATAVIHKLHNSGFKVVALEIEEPLAIRRQIS
                     FCEAVYEKKVTVEGVECIFCNTEKEIEEVLSQHKVALIIDPKGEKLSKLNPIVLVDGI
                     LAKKNLGTTKDMAPLTIALGPGFCAGEDVDVVIETMRGHNLGRIITNGRAEKNTGVPG
                     AIKGFSKERVMHSQYDGIMENVCKIGDIVEKGDLLCYINNYEKKHEVRATISGVLRGL
                     LKDGAKVNKNLKILDIDPRKEEVKNAFTISDKARCIAGGVLEAILNNGIYPQN"
     gene            78552..78989
                     /locus_tag="PC471_000183"
     CDS             78552..78989
                     /locus_tag="PC471_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCMP deaminase"
                     /protein_id="HDF2787331.1"
                     /translation="MRPSWDEYFMEIAEVVKKRSTCIRRQVGAVIVRDKQILTTGYNG
                     SPRNLEHCENIGCKRQELNIPSGERHELCRALHAEQNAIIQAAHNGISVDGATLYVTT
                     RPCVLCAKMCINAGILKIVYEGDYPDDMSTELLKEAGIELIKF"
     gene            79174..79401
                     /locus_tag="PC471_000184"
     CDS             79174..79401
                     /locus_tag="PC471_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HDF2787332.1"
                     /translation="MSKRNTHTEIAKLFSLISQIGLMIVISILGCTFLGKFLDSKLNT
                     TPVLTIIFLLLGVGGAFSGVYKTLIVYTRRK"
     gene            79406..79792
                     /locus_tag="PC471_000185"
     CDS             79406..79792
                     /locus_tag="PC471_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit I"
                     /protein_id="HDF2787333.1"
                     /translation="MDIKLLNQVRSVTKGIVIFDVVLIVLMLITSTLSKPVLLGLIFG
                     SIIAMLNFRLLAISLEKSVTFPAGKAQAYASAQYAVRLFIMAVVLIASATVPHLNIIG
                     TALGLLSTKFVILSKNFINKLKRKEA"
     gene            79795..80499
                     /gene="atpB"
                     /locus_tag="PC471_000186"
     CDS             79795..80499
                     /gene="atpB"
                     /locus_tag="PC471_000186"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HDF2787334.1"
                     /translation="MNQAHYYLIGNFKISETILVSWGIMAFLAIASYFLTRNLKKVPT
                     SKVQIFLEFAVGGLAKMVTDVMGKNTVKRMPYMIPYIGSLFLFFACSNLSPLLGLRSP
                     TTDLDTTAAWAMITFFMIYFAGVKFKGLSYFKELAQPLAIMLPINIIGELARPISLSF
                     RPFGNILGGTIIMGLFYQLLGFISSLIPGLSIPIGEILIPVPLHLYFDLFAGLLQAYI
                     FIMLTMVFISSAGELD"
     gene            80568..80828
                     /gene="atpE"
                     /locus_tag="PC471_000187"
     CDS             80568..80828
                     /gene="atpE"
                     /locus_tag="PC471_000187"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HDF2787335.1"
                     /translation="METAIVAAASAIGAGIAVATGIGAGIGQGIAAAKAAEAVGNQPE
                     AKGDITSTLLLGVAIAESSAIYGLVISIILLFVNPFFKYLGM"
     gene            80951..81448
                     /gene="atpF"
                     /locus_tag="PC471_000188"
     CDS             80951..81448
                     /gene="atpF"
                     /locus_tag="PC471_000188"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HDF2787336.1"
                     /translation="MEKALVGITWEFVFQIVNTFIIFLLLRKLLFKPVLNIIESREND
                     IKSDLAEGEKAKNEGLALKKEYESKINFAKDEGQEIIKQATIRAEQKSDDIVNTAKKD
                     ALDIKEKANKDIEQERQKVINEIKNDISNIALLAASKVIEKDLDKSKHEELIENFIKE
                     VGEAK"
     gene            81445..81990
                     /locus_tag="PC471_000189"
     CDS             81445..81990
                     /locus_tag="PC471_000189"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HDF2787337.1"
                     /translation="MINVIANRYAEALFQLGEEENSTDVLFKELEKVVDMMTKVSKDF
                     YKVLKSPLVSKSEKKNLVEIIFSKEVSSNIKNFLKVLVDKDRISYLEDIELAYKELLN
                     KKNNVIDGVAISAIPMSETDIKELEVKLSNKYNKNVTIENVVDKTILGGVLVRIGNEQ
                     IDGTVKTRLDKMKEKLSEVIS"
     gene            82007..83509
                     /locus_tag="PC471_000190"
     CDS             82007..83509
                     /locus_tag="PC471_000190"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003405060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HDF2787338.1"
                     /translation="MNLKPEEISSIIKQQIKNYENKVELTDTGSVLTVGDGIASVYGL
                     EKAMSGELLEFPGEIYGMALNLEEEVVGAVILGDDSEIKEGDIVKRTGRIVEVPVGEA
                     LIGRVVNSLGQPIDGKGPIAYTKTRPVESEAPGIIDRRSVYEPLQTGIKSIDSMIPIG
                     RGQRELIIGDRQTGKTSIVIDTILNQKGKDVICIYVAIGQKRSTIAQLVSSLEKGGAL
                     DYTIVVSATASESAPLQYIAPYAGAAMGEEFMYNGKHVLIVYDDLSKQAVAYREMSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDELGGGSMTALPIIETQAGDVSAYIPTNV
                     ISITDGQIYLQPELFYSGVRPAVDPGISVSRVGGSAQIKAMKKVAGTLKLAYSQYREL
                     AAFSQFGSDLDEDTKKRLAQGERIVEILKQGEHQPIKVENQVMIIYAVINNHLEDIPI
                     DNIARFESELYAFVDNNYPEISRKILGGEDFTHDLTDAINEFKEKFVVEV"
     gene            83537..84415
                     /gene="atpG"
                     /locus_tag="PC471_000191"
     CDS             83537..84415
                     /gene="atpG"
                     /locus_tag="PC471_000191"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HDF2787339.1"
                     /translation="MAGAGIKAIKTRMGTVESTKQITKAMELVASSKLRKAKEKAEDS
                     REYFNTLYNTVKDIAQTTRGIRNEFLKEREVKNKCYVVVAGDRGLAGGYNSNVLKESV
                     SHMKGKKETVITVGKKAHEFFSKRNYNIAKDINSVEACGYEDATEISQTIIDLYKKGE
                     VDEVYIAYTKFKSALSQEVEILKLLPLVFEKDTDEQGMAVKEEKQNRSKVQYIPSAEA
                     VLSYIVPQFISGTVYGGILESFASEQGARRTAMESATDNANEMLSNLELSYNRARQSA
                     VTQEITEIVGGVEALK"
     gene            84428..85822
                     /gene="atpD"
                     /locus_tag="PC471_000192"
     CDS             84428..85822
                     /gene="atpD"
                     /locus_tag="PC471_000192"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HDF2787340.1"
                     /translation="MANVGKVVQIVGAVLDVKFDSEQSLPNLLNALVIKLGDKEIVAE
                     VAQHIGDDTVRCIAMSATDGLVRGMEVVDTGGPISVPVGDETLGRIFNVLGKPVDGKP
                     APKSAPKLPIHRPAPAYDELETTAEILETGIKVVDLLAPYLKGGKIGLFGGAGVGKTV
                     LIQELINNIAKQHGGISVFSGVGERTREGNDLYGEMSESGVINKTALVFGQMNEPPGA
                     RMRVALTGLTMAEHFRDEQGQDVLLFVDNIFRFTQAGSEVSALLGRMPSAVGYQPTLA
                     TEMGALQERITSTKKGSITSVQAVYVPADDLTDPAPATTFSHLDAKTVLSRQISSLGI
                     YPAVDPLESTSRILDPSIVGKEHYEVARGVQSILQRYKELQDIIAILGMDELSDEDKL
                     IVARARKIQRFLSQSFTVAEQFTGNPGQYVPVKETVRGFKEILEGKHDDLPESAFLFV
                     GTIEDAVRKAKGSM"
     gene            85825..86085
                     /gene="atpC"
                     /locus_tag="PC471_000193"
     CDS             85825..86085
                     /gene="atpC"
                     /locus_tag="PC471_000193"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit epsilon"
                     /protein_id="HDF2787341.1"
                     /translation="MASEFSLKVVTPSNTFYDGDVEMIVVRTTKGDKGVLKGHIPFVS
                     GIPVGTLKIKKEGSFKEAKIAEGFINVTQDKTVILTESAEWI"
BASE COUNT        34784 a         9324 c        15863 g        26384 t
ORIGIN      
        1 tattaaaaac ttaatagaaa tattaagcat aaagattatg tggttacaat agcagagagg
       61 atacacctgt tcccattccg aacacagaag ttaagctctc tagcgctgat ggtacttggt
      121 gggaaactgc ctgggagagt aggacgtagc cacgtaattt tttttatttt ttgtcaaaaa
      181 aaacaaaaaa tacgtatttc ttttttcatt tcgggaaaac taaacacagg aattatggac
      241 tttataaaaa tttaattctt gaggagtgat ataagtgaaa aaagttgctt taattttagc
      301 tataataggt gcgcttaact ggggtgcaat tggtattcta ggtacagact taataggtag
      361 tatctttggt ggaacatatg aaatggtaag tagaataata tacttcattg taggtttagc
      421 tggattatac ttaataccaa gtttaatgtc tgatgataga gaataattta tgcttttatg
      481 ttaaatacct cattctaaat gtttagaatg aggtatttaa ttttataaaa atattaattt
      541 gtttcttttt taatggtaaa atagataaga attgaagttt taaaaagaag tattatgtaa
      601 aaaaggagta ctattaatat atgtttatta ttaatgaatt aaataagatt gtaaatgaag
      661 aagtaatatc ttttcatatg cctggtcata aaaagggaca ggtttataaa aagttaggat
      721 ataaagatat actaaaaaat ttgtataaaa tggatacaac tgagatatta ggaacagata
      781 atttacactt tccggaagga attataaaaa attcacaaaa aaaagcatca agagtattta
      841 aaagtgatta tacttgctat ttagtaaatg gtagtagctg tggaatacaa tctgcaataa
      901 tgtcagtatg tagtcctaaa agtaaaataa taacaaatag agactgtcat cagtctgtta
      961 taaatggatg tattttagga gatatagata tagagtatgt gccttgtgag ataagtgaag
     1021 agacaaatgt cttaaaaggg atatctataa gtaatgtgat tgatataata gataaaaact
     1081 tggatgcaaa agcagtactg cttacttatc ctacatatta tggaatgaca tatgatttag
     1141 aatacatatg taattatgca catagcaaaa agatggtagt gatagtagat gaagcacatg
     1201 gtgcgcattt agggcttagc gagaggcttc ctaaaacagc tcttgagcaa ggcgcagata
     1261 tagttgtaca gagtacacat aaaactcttc cagcatttac acaatcgtca atgatacata
     1321 tacagggaga ccgagttgat atagataaaa tattgaacat gttaagaatt acagaatctt
     1381 caagcccttc atatttatta ttatcttctc tggaattagc agtagatata tatgaaaata
     1441 aaggaaaaga attaatggaa gagcttttaa ataatattat aactttcaaa aataaagttg
     1501 attcaaatat taatatatac gacacaaatg ataaaacaaa aatatttata tcatctaaaa
     1561 atatgggttt aacaggttat gaactagaaa atattttaag aaacaaatat aacatacaag
     1621 tggagttatc taattattat ggagttcttt taatatgtac aataggaaat actgttgagg
     1681 atttctctag tttagaaaca gcattaaaca atattgtaaa ggaagacttt aaatttgaaa
     1741 agttagaaaa taaaaaatac cctgttgtta ttccaaagaa aatattaaca cctagagaag
     1801 cattttataa aactaaaaaa agtgttaaaa tatatgacag cataggtaaa gtttgtggtg
     1861 aatgtatagt tccataccca cctggaataa gtgtaatatc tcctggtgaa gtaataagta
     1921 aagaaataat agactatcta atgttttgtc attctaaagg catgattgtg agtggattga
     1981 aagatgtaga tttaaatttt atacaaataa tagatttaga agacatttaa aaattatgat
     2041 ataaaagtag atagaagtag atagtttaaa attgtgaaga gaaataaata gactgcttat
     2101 atagagctat ctaataaaag tactaaaaaa gtaaaaagtg tttgtatgga ggatatgaaa
     2161 atgaaaggta agttaataat tatagagagt ggttcagatg caagtggaaa ggctactcag
     2221 acagaaatat tatatgaaag attaaaaaaa gaaggctata tgattaaaaa ggtagaatat
     2281 ccaaattatg aatctccatc atctgccttg gtgaaaatgt acctaaaagg agactttggt
     2341 aaaaatgcat cagatgtaga cccatatata gcatcaactt tttttacagc tgataggtat
     2401 gcatcattta aaaccgattg ggaggagttt tacaatgaag gtggaataat tatcgcagat
     2461 agatatacaa cctctaatat ggtacatcag gcctctaaaa tggatatttg tgatagagaa
     2521 gagtatttaa attggttagc tgattatgaa tttaatttat ttaagatacc acaacctgat
     2581 tgtgttatat ttttagatgt gcctattgaa tttagtaaaa agcttatgga gaatagaaaa
     2641 aataaaatta caggggaaga aaaaaaggat attcatgaaa gtgacataga gtatttagag
     2701 aaatcttaca acaatgcttt atatatagca gataaatatg attggaaaaa aataaattgt
     2761 gttttagata acaaattaag aagtattgaa agtatacata atgaaattta taaaatagtt
     2821 cttgattcaa taaaacctat ggagagataa atatgtattt tgaaaatatt atagggcaag
     2881 attttgcaaa gaaatattta acaaattcta taaagaaaaa taaacttaat aatgcgtata
     2941 tgtttgaagg aatggatgga ataggaaaaa agaaatttgc agatgaatta tcaaaactat
     3001 tacttgatta tgaaaatctc gaaaatagtc cagactatgt actcataaaa ccagatggaa
     3061 atagtataaa aatagctcaa atcagaaatt tacaatcaga tatagtaata agacctcata
     3121 aagactataa aatatatata atcaataatg ctgaaaaaat gactgtggaa gctcaaaatg
     3181 cacttttaaa gacgctagag gaacctccaa actatgctat aataatctta gtcacaaaca
     3241 acaaggaatc tttattagaa actataaaat ctagatgtga tataataaag ttctcaccta
     3301 taccaattga agacttaaaa cgttatttga taaatacagg tatagaagaa gaaagagctc
     3361 aattattggc tattttttcg agaggaagta ttgagaatgc tctaaatcta tctcagtctt
     3421 ctgagttttc aatgatgaga gaagatatac aacagtatat tcagataatg ttagataaaa
     3481 acatagtgga gattttaaat ataccaaata atatggaaaa atatagaggt aagattatag
     3541 ctttattaga tatgatgata aactattttc gagatatcat cctattaaaa gaaaatgtaa
     3601 acaagaacat gttaataaat gtagataagc tagttttcat tcaaaatatg agtggaaaaa
     3661 ttagttattc tcaattgtcc aagattattg atataataga agatgcaaag agtaaaataa
     3721 aaagcaattg caatttcaat ataagcatac aagttatgtc tttaaatata tacgaggtga
     3781 ttaaatgata aagatagtag gtataaggtt taaaagtgct gggaaaatat attattttga
     3841 ccctgttgat tttaatatag agcaagatat ggatgtagtt gtagaaactg caagaggctt
     3901 ggagtatggg aaagtagtcg ttggtccaaa agatatggat gaaagcgaat tagtttctcc
     3961 tttgaagcca attataagaa tagctacaga agaagataaa aaagtatatc tagaaaataa
     4021 ggaaaaggca aaagaaactt ttgagttgtg ccaacaaaaa ataaaagaac atgagttaac
     4081 aatgttttta atagattgtg agtatacatt tgataggaat aagttgattt tttactttac
     4141 agcagaagga agaatagatt ttagagaatt ggtaaaagac ttagctgcta tatttaaaac
     4201 tagaatagaa cttagacaaa taggtgtcag agatgaagct aaatctatag gaggtctagg
     4261 gccttgtggt agaaaacttt gttgttcatc ttggttaggt gattttcaac cagtttcaat
     4321 aaaaatggca aaagaccaaa gtctatctct aaatccaaca aaaatatctg gaatatgtgg
     4381 gagattattc tgttgcttaa aatatgagca tgatgtatat agtgaagcaa tagaaaaaat
     4441 gcctgttgta ggtgcattgg tgcaatcaga tgatggaaaa ggaaaagttg tagaagtgaa
     4501 tcctttatta gaacaaatta aagtggaatt tcaagacaag acagtaaaga tatacacaag
     4561 agaagatata aaaatactta gagagcctaa aaaatgtgat ggctgtggtg gtaaatgtaa
     4621 ggataatgat ggattagatg aagccacact aagagagcta aagaaattag aagattaaat
     4681 ttaaggtagg gcttttccta ccttttttaa agcaattaat gcttaaagtt attaatattt
     4741 gaggagaatt tatatatgca attaaaagat tcagaaagaa tagatgattt acaattaaaa
     4801 ggccttaaaa ttatacaaga tactaatggt ttttgttttg gtatagatgc agttttatta
     4861 gccaatttta ctaaaataaa aaaagatgcc aaagtagtag atttgggtac tggaactgga
     4921 attataccta ttttgatagc tggaaaaagt gaagctaaaa aaattattgg cgtggaaata
     4981 caagaagatg tatatgaaat ggcaactcgt tctataaaat taaatggatt agaagaaaga
     5041 gttgagatta taaatgaaga tataaaatac atagacaaag tcttagacat aaatggttac
     5101 catgtagtca catcaaatcc tccatatatg catattgatg gaataaaaaa tccaaatgat
     5161 aaaaaagcaa tatcaagaca tgaagtaagg tgcaatttgg aagatgtaat tagagctgca
     5221 tccagattgg ttatgcctag aggaaaattc ttcatgatac atagaccaac aagattggtt
     5281 gatataataa ctttagggag aaaatataaa ttagaaccaa aggttattca atttgtgcat
     5341 ccaagacctc aaaaagcacc gaacttagtt ttggttcaat ttgtaaaaga tggaaggcca
     5401 gagcttaaaa tattggaccc attgtatgtt tatggagaag atgggaatta tacaaaagaa
     5461 ttaaaagcaa tatataataa taaagatata ggagagttgt aaatgagtgg gaaattgtac
     5521 atatgtccaa caccaatagg aaacctagaa gatataacat atagaacttt aagaacatta
     5581 aaagaagtag acttgattgc agcagaagat acaagacata gtataaaact tttaaatcat
     5641 tttgaaatct caaaaccact aactagttat catgaacata ataaggattc aaagggagat
     5701 tatttgataa ataaactaat tgatggtgaa aatatagctc tgataagtga tgcaggtatg
     5761 cctggtatat ctgacccagg agaagagata ataaaacaag ctatacaaaa taacatagag
     5821 atagaagtat tgccaggtgc aacagccttt gtaacggctt tagttggctc tggtatggac
     5881 acacatagat ttgtttttga gggcttttta gatagagata aaaaagttag gaaagcacaa
     5941 ttggaagaat taaaggaaga aagtagaact ataattttct atgaatcacc acatagattg
     6001 aaggatacac taaaagatat gcttaaaatt cttggtaata ggaatatttc tataaataga
     6061 gaacttacaa aaaaatatca agaaataata agagaaaata tagaaactac cataaaaata
     6121 tttgaggaaa aagaaataag aggagaattt gtattaatag tagagggttt tcatggtgag
     6181 aagtcaaaga aagaaaatta tgatgggctt acagataggg aatatgtttt aaaactaatt
     6241 gaaagtggta tgaacaaaaa agatgcaata aaaatagttt gtaaagatag aaagttaaag
     6301 aaagatgtgg tttataaaca agttttagat ttataaaacc tgaatctgag gtgattttat
     6361 ggaaataggg aaaattataa tagagagttt aaagagggaa gatacacata aaatatttat
     6421 agaattagat aataagcatg aattagaaaa aataattcct aaaataagct gtatgaagtc
     6481 tgtaggtgaa tgtaaatatc atgtagtaaa ttgttttgaa cattctataa acgcattaaa
     6541 agaattagaa atagtattaa atgataagga tttttttcca atacatctaa gaaaacatgt
     6601 gtcaaactat ttaaacacat atattgaaga aggaataaat aagttacatg tattaaagct
     6661 gggaacattt ttacatgata taggtaagcc tgacagtatg accttagatg aaaccggaag
     6721 agttcatttc acaaatcatg aaaaaatagg tgctcaaata attgacaata tgggaataaa
     6781 gttagagtta tcaacagaga catatagact tataagtaaa tatgtaagat atcatatgat
     6841 acttttgtct ctatataaga aaaatgattt gagtaggaaa gaattaatta atgtatttaa
     6901 cttagtagat gaggacacta taggtgtaat aatgcttggg tatgcagata tagtgtctac
     6961 agtaaaatta ttgggcaaga gtggagaagt cagtgtgtta aagacatata tggaatatat
     7021 tttaacaaat tacttgtata agagtaatta tacacatgta taaatttagt taatataatt
     7081 gagttttaga tgattaattg aagtttgaaa tatacggatt tcaaaaacat aaaagaagtt
     7141 gtctttgttg taaaattatg aaagacaact ttttttatat caaaaattga aattttatta
     7201 aggatactta atggtctatt aattaataaa ccattagtat ttaatttata ttacaatatt
     7261 gattttgctt gtattggcgt tgatagtata acagaggtct tagtagaacc aattttcttt
     7321 atagtatcaa tcacattttc taactcgtac atatctttta caattacttt taaaagtaag
     7381 caatcatcac ctgttatatg gtgacattct acaatcctag ggtcctttgc agctgactca
     7441 ataaattctg tatatccatt gcttggaaga gaaatatgaa taaatgcctt tataactctg
     7501 cctaatgaat ctgggttgac aatagcttta tatccttcta taacaccaga ctcttctaat
     7561 cttttgactc tttctgaaac tgcaggagaa gttaaaccaa ctatttttcc taagtccttc
     7621 atagaaattc tcccatcatc ctgtaaaatt tctatgattc tgtaatctgt aacatccata
     7681 aataaacagt cccccttaaa taaaaataat taatgctata aaactttttt agaatctatc
     7741 atttgtgttt ttaaatacca aagatttcta gataaatcaa ccttataaat gtgtggtttt
     7801 gaaagtcttt tgtattgttc ccagatagta cttaattcat attgtacatg attttttatt
     7861 tcaagaacag cttttttatt atatttgcat ctgcctttta tatacaatgg tttaagaagc
     7921 tctttaactt tgtaatttgt aaataccttc gttttccatg tataagtagg atggaagatt
     7981 tctaaaggtt tagactcatc tataacttca tcatgtaaca taatcaaatc tgcctctgcc
     8041 ttattgtctt cgttatatat tcttataact ttcttgtaac caggattatt tattttttct
     8101 ggattctcag aaatctttat cttaggttca agaactcctt tttcacaaga tgcagctaat
     8161 ttataaactc ctccaagaga aggtgaatcg aaagatgtaa tgagctttgt acctactccc
     8221 catgaattta tagtagctcc ttctgcttta agtgatgtta tggtatattc gtctaggtca
     8281 ttagatgcag tgatgctgat atctgaaaag ccagcattat ctaattgttt tttagcttct
     8341 ttagataaat aagctaaatc tccagagtca agtcttatac ccataggttt gtatcctttt
     8401 tcagaaatat ttttgaatac ttttattgca ttaggaactc cactgtttaa tacatcataa
     8461 gtatctacaa gtagtaggca tttatctgga taaacatcag catatgcttg gaatgcttca
     8521 agttcattat caaatttttg tacccaactg tgagcttgtg taccaattat aggtatatca
     8581 aacattttcc ctgctagtac gtttgcagtt ccagcacaac caccaactac agcagcccta
     8641 gccccatata tccctgcatc tggaccttgt gcacgacgta atccaaattc aaatacaggg
     8701 tctccttgag ctgcaaagca aactctagag gctttagtag caataagaga ctgaaaatta
     8761 actattgtta acaatgctgt ttcaataagt tgagcttgat ataaaggtgc cttaacagta
     8821 attaatggct cattagggaa catgatagtt ccttcctcaa cagcatatat atctcctgtg
     8881 aatttaaatt cttttaaaaa ttttaagaac ttgtcagaaa ataaatttaa gttttttaag
     8941 tactctaaat cttcatctga aaaatgaaga ttatctatgt attcaacaac ctgatcaata
     9001 ccacaaacta tagtataccc tccatcacaa gcgttttttc taaaaaacat atcaaataca
     9061 acaatgtctt catgaatatt cttttcaaaa tagccattta acatagtaag ctgatataaa
     9121 tcagtaagaa gtgttagatt tctcattatt tctctcctta attattatta aatattaaaa
     9181 attgtaaaaa ctcccacact tatactaaaa cataatatag tacaactttc aagtataaag
     9241 tattacctaa ctatcataaa attatttaaa gaggaggatg attataatag gaaaaatatg
     9301 gttttatatg gtatcgatag gaataatagg aagcttgatt tctaaaaata tgggtgaatt
     9361 aaataaagtt atattaagtg aaacatcaaa aggtatagag tttgcaataa gtctagctgg
     9421 tatcatggca ctctggatgg ggataatgaa tatcgcaaaa gaatcaggtt taattgaaaa
     9481 gataggtcaa aaactaaatc ctataatgag aagattattt ccttctattc caaaaggtca
     9541 taaagcaatg tcttatatag ttatgaatat tgctttaaat atgttgggtg ctggaaatgg
     9601 tgcaacagca tttggtttaa aagcaatgag tgagcttcaa actttgaata acaagaaaga
     9661 tacagccact aatgatatga ttatgtttat ggtaatcaat atatcatcta tacaaataat
     9721 tccatttacc ataatcaaac taaggatgga tatgggggct caaaatccaa gtgaaataat
     9781 ttttacgact ttatttgcaa ccataatatc tacagcagta gcaataataa catgtaaatt
     9841 ttttcaaaga agatatagat aaaggggatg tagatacatg gagatatttt caaacttact
     9901 tataccaata ataattctgt acatagtaat ttatggtaaa tataaaaaaa tagatgtgta
     9961 tgatagtttt gtaaaaggag caattgatgg attaaaagct gcatgggata tcctaccata
    10021 tataataggt atatttttgg caataggaat attcaaaact ggtaaaggtc ttgatatgtt
    10081 agaatggcta tttacaccaa tagcaaatat gatgagtata ccaaaagaat taataggtct
    10141 aataagtgta aaacctttat ctggaagtgg tgctttaggg atgtacagtg aacttgcaaa
    10201 tagagttgga ataggaagtt tagtagaaaa aatgggtgct acgattgtag gttcttctga
    10261 gactattttt tacacaatgg caatatacta tggaagtctg aagataaaaa atactagaca
    10321 tacattatca tgtgctatga ttagccatgt tgcaggtgtc atagcagctg tttttatttg
    10381 ctatgtaata tttgtataat ttgttgacaa tatgggaaaa attttataat atagtgatta
    10441 tatagaaaca aatagtaata caaattacaa gctatgaaaa gaagagtaaa taaaggtttt
    10501 ttctatcaga gagctaggtt aggtgaaagc tagtgtcaaa agctttattg aagatggtct
    10561 tggagctttt tatctgagca agtgtgctag ggtaaaacgg ttggacacgt tatagtctac
    10621 taaggtatct ataaaatgtt tctacatttc atgtgaaaca taactattat agatgaatta
    10681 gggtggtacc gtgaataatc tcgcccctat gttaactcat aggaggcgat tttttattat
    10741 ttaaatgaaa gaatcatatg attacgaacg ataaaaaatt aggaggtaaa taacatgagc
    10801 aaaccgagtt tttatgtaac aacaccaata tactatccaa gtggaggatt acacatagga
    10861 catacttatt caacagtagc tgcagataca atagctagat tcaagcgttt ctgtggatat
    10921 gatgtaaagt tcttaacagg aacagatgag catggtgaaa aaattcaaaa gaaagccata
    10981 gaacaaggaa tgtcagagat agagtatctt gatggaatga taaaagatat aaaggcctta
    11041 tggaatacta tggatatatc ttatgatgat tttataagaa ctacagaaaa aagacataca
    11101 gacataatac aaaagatatt tactaaacta tatgagcaag gtgatatata taaaggtgaa
    11161 tatgaaggta gatactgtac tccttgtgag agtttctgga cagaatctca attactagaa
    11221 ggaaataaat gccctgactg tggaagagaa acatatttag taaaagaaga atcttatttc
    11281 tttagattat ctaaatatga agacagatta aaagagttat ttaaagatga tagtttctgt
    11341 tttcctgctg ctagaaaaaa tgaaatggtg gcaaactttt tagacaaagg attagaagat
    11401 ttaagcgtaa ctagaacgac ttttgactgg ggtataaaag ttccttttga tgaaaaacat
    11461 gtaatttatg tgtgggtaga tgctttatgt aactatataa cagcattagg ttatatgact
    11521 gataatgatg aagaatttaa aaaatattgg cctgccaatg ttcaaatagt aggtaaagaa
    11581 atagtgagat tccatacaat aatatggcca gcgcttttaa tggctttagg tctagaagtt
    11641 ccaaaacaag tatttggaca tggatggata ctatttgctg atgataaaat gagtaaatca
    11701 aagggtaatg tggtttatcc agaacctata atagaaagat atggaataga cacacttaag
    11761 tacttcttat taagagagtt tgcatttggg caagatggaa gttatactca tagaaatttt
    11821 gtaacgagaa taaattatga tttagcaaat gatttaggaa acttaataag tagaactgta
    11881 gctatggttg aaaaatacaa taatggtata ataccaacag caaaagtttc aactgatttt
    11941 gatgctgact taaaagaaca agctgtttca actagagaaa actttgaagc tgaaatggat
    12001 aaaatgcaat tccatgaagc attagagagt gtatggaaat tagttagaag aactaacaaa
    12061 tatatagatg aaactatgcc ttgggcacta gctaaagatg aaacgaagaa agatgaatta
    12121 gacacagttt tatataattt atgtgaatct ataagaataa tcgcaacact aataaatcct
    12181 ataatgaatg agactgctaa taaaatatat gaacatatag gaataaaagg tcaagatgat
    12241 ataactacat gggaaagtac aaaaacattt ggtcttattg gagaaaatgt aaaagtattt
    12301 aaaggtgaac ctttattccc aagattagat gtagaaaaag agatagaaga attaactaaa
    12361 atgttctctg ggaaacctcc agtagaagaa aaacctttag agcataaaga agaaattact
    12421 attgatgatt tagacaaaat agagcttaga gtagggaaaa taataagctg tgaaaaacat
    12481 ccaaaagcaa ataaattatt agtatcacaa gttaagattg gaccagaaac aagacaaata
    12541 gtatcaggaa tagctgaata ctataagcca gaggatttag taggaaaaga ggttacagtg
    12601 gtatgtaact taaaaccagt taaattaaga ggtgtagaat ctcaaggtat gatattagca
    12661 gcaggtgatg atggagaccc atatgtttta ccatttacac aaggggctaa agatggatgc
    12721 gaggttcgtt agtaggtaat taaatagtaa ggagattaaa gatgttattt gactcacatg
    12781 cacatttaaa tgatgaaagt tttgatgaag atagagatga acttattggt tcattaaaag
    12841 ataaaggtgt agatttggtt gtaaatccag gagcagatat agagacgtcg ataacagcaa
    12901 ttgaacttgc gaaaaaatac gactttatat attcagcagt tggggtacat ccacatgatg
    12961 tatcaaaact agatgataca gcaatagaaa ctctaagaaa acttgcaact gagaatgaaa
    13021 aagttgtagc tattggagag attggattag attattatta tgattattct ccaagagaag
    13081 aacaaaaaga gtggtttaag aagcaaattg aattagctaa tgagctaaaa cttccaataa
    13141 taatacatga tagagatgca catggagata cttttgagat aataaagaat actaaaaatc
    13201 ctgaaatagg atgtgtactt cattgttata gtgggaatgt agagttagct agagaatatg
    13261 ttaagatggg ttgttatata tcaattccag gcacggttac ttttaagaat aataaaaaaa
    13321 ctagagaagt agtaagagaa atacctctag aaagattatt catagagact gattcaccat
    13381 atatgtcacc agagccacat agaggaaaga gaaacaatcc ttcccaagta tcttttgttg
    13441 ctgataagat agcccaggaa aaagggatat cttatgaaga agtatgtaga gttacaaaag
    13501 aaaatgcaaa gaaatttttt aatataaaat agacaaatta aatcttataa tgatatatat
    13561 aataagtcat tataagtata ggatgagagt gcctcaaaat gagtttttta gttcatgagg
    13621 cactcttttt tgtatgaaat caaatatatt ttcatcattt caacaatgaa aaagaagttt
    13681 atcatatttt gagatagttt gtttttattt atttaattca tattttaaaa tatattttct
    13741 taacattgtt aaaaaagaat ttacaagaga ttcattagaa ttaactcaac tctaatattc
    13801 acttaactta agttgtttat tatattatta aagagaagta aacgaaaggt agattaagga
    13861 ggttgaattt tgtatacagt tgatagtact aagatattga taaatatgaa cttagagaaa
    13921 ctagaaaaga tatactcaat gataaaagat acatcaagta tttctataat taaagaacct
    13981 aacttggcta cagtaatggt aagagctaat gaaagtgtaa aaaacacaac atttaattta
    14041 ggagagatat tagttactga atgtagtgta aaggtagatg aaagcttggg ttacggaata
    14101 gtttcagaaa acaataataa gaaagccatt tatttagcag ttatagatgc agttttacat
    14161 agcaataaca gtaaatttga tgagttaaag aattacataa ataaaagtgt atatgaagag
    14221 aacttaaggt atgaacaaga gattattgat gaatttagct tgattaataa aacgaaagtt
    14281 caattcaatg ctatggatta aataggagga gaatcatggt aaattataca caagaaatat
    14341 atagaaagat gctagatagt gcttcatttc ctgggaaatt aaataatata gataatataa
    14401 atatagaaaa taatacaaat ctattgaatg gagcaattgg tatagcaatc actttattag
    14461 accaagaagt taccttctat atagaaaatt atactcaaaa agatataaaa aatataagag
    14521 cacttacaat gtcaaatcaa atagattata gaaaatcaga ttatatattt ttagatatta
    14581 attcagaagt ggatatatta cagattaaaa taggttcttt tgaatatcct gatgaaagtg
    14641 caactatagt acatcaagta aatgatttat caacgtactc tcaaactaag tatataaagc
    14701 ttgatttgag tggtcctggt ataaaatcta aaaatagtat atatataaat ggaattagag
    14761 aagaatttat agaaagatta tctagtataa ataaagatta tccaatagga atagatttga
    14821 ttttagtaga taaaaaagga gaaattgcat ttataccaag gtcatcaaaa ttaagttggg
    14881 aggtattata gaaatggctt atgtagcagt taaaggtgga gaagaagcca tattacaggc
    14941 taaaaatata cttgagtttt atagggtaaa aggagatagc ttgcctatag atgtaaagca
    15001 gattgaggaa caaatgtctt atgctgtaga caaagtaatg tctgaaggaa gtttatacaa
    15061 caaggaacta tcagcattgg ctataaaaca agctcaaggt gatatattag aagcctcttt
    15121 ctatgtaaga gctttaaggt caacaatgcc tagaattaca tattcagaac ccataaatac
    15181 agaagaaatg agaataaaaa gaagaatatc agctactttt aaagatatac caggaggtca
    15241 gtatcttggg gcaactagag actatgaaaa tagaatattg aatgtagaat tattagaaga
    15301 agatgaaaaa aagaatagac attttagaga tagttttata aatgaattag aaacagtaga
    15361 tgaaatccct gaatttacaa aaatatctaa tatatttaga aaagaaaatt tattacaaga
    15421 agaagtttgt gaaaacaaat tggaagaagt tattgatgat attacaagaa atagtttaag
    15481 atttccagtt tctagggcag caaagttaca ggcattaact agaggtgaaa gcggagtagt
    15541 atcaggtttt gcttatgcta ttgtaagagg ctttggtgat gaacatccat atataagtga
    15601 acttagaact ggcaatgtgt ttataaaaat aaaacatccc atctatgaag atgaagaaat
    15661 aacaataggt gatatgttgg ttactgaatg tgaaatagtt tcaaaagcat cgcaaaataa
    15721 gactgaaaat tctaaaaata attcggaaga taaaaataaa atgtctttag gatatggtct
    15781 ttgtatgggg aacaatgaga ataaggctat atctatggct atactagaca aatctttgga
    15841 gccaaatagt gaaaaactag cacaagacga agaatttgta ttgcttcata tagatggaat
    15901 agattcatta ggatttatat ctcactttaa gcttcctcac tatgtagatt ttaaagcgga
    15961 tgtagaaaga ataaaaaagt ttgaggaggt aagtttaggt gatatataac tatggttttt
    16021 tagacgaaaa cacaaaaaaa gaaataagaa gaaagatatt aaaagcaata tcaatacctg
    16081 gttatcaagt tccctttggt tcaagagagt tgcctatagc taagggatgg ggtactggag
    16141 gtttacaatt aacactatca ttaattggta aaaatgatgt agtaaaagta attgaccaag
    16201 gaagtgatga ttctacaaat gcttgtaata taaggagctt tattgcaagt gtaagtaatg
    16261 tagaaaccac aaaagataca ttagaagcta cattgattca aacgagacat agaataccag
    16321 aagaaaaact taaatctaat caaatactaa tatttcaagt accaatacca gaaaccctta
    16381 gaatagtaga acctagtgaa gtagaaacaa gaagaatgca ttcagaagaa gattatagta
    16441 ggatgtgggt atatctttat gaagatatag ttagatttaa tgatatatct atagcagttg
    16501 aatatccatg taaagtaaat gatagatatt taatgaatcc atcacctata cctagatttg
    16561 atataaaaaa attaaatatg tcagacaatt tattcttgtt tggtgcaggt agagaaaaaa
    16621 gaatttatgc tattcctcca tatacaaagg tagaaccttt agagtttgag gattataagt
    16681 ttgaagagga aaagtttgaa ggaaaatact gtagtttatg taacagtaca aatacatttt
    16741 tagatgaagt atatgacagt gacactaatg aaaagtacta tagttgctca gatacaagtt
    16801 attgtgaaaa agttagatta aaaaataaca gtatagatgt gacaatagga ggtacttgga
    16861 atgagtaaaa taaatgactc taaaagatta gaaaatatag acaagcaagt attaatagga
    16921 agaaatatca atcttattta tggagatggg tgtgaaaagt gttttgaaag cacaggatat
    16981 gaaagcaata atatatgtaa gtattgtggt tctatagttg catgtaataa tgtaaattta
    17041 gaactttatg aaggagaagt ccttggaatt gtaggtgaat ctggttcagg aaaaagcact
    17101 ttattaaaaa tactgttttt ccaagaacat gcaaatagtg gagaagtata tatatcccat
    17161 tacaataaag acacaaatat actagagctt tcagaccaaa aaaagagata cataaagaat
    17221 cattttatgg gtattgtgta tcaaaatccc caccttggtt taaatttaga ctttagtagt
    17281 ggtggtaata tagcagaaaa attattgatg gcaaatttat ataatgttga aaaaataaga
    17341 gatagaggca aagaactttt aataaaaact aatatacctg tagaaagaat agaccataaa
    17401 ccaaaatact ttagtggggg aatgcaacaa cgagttcaaa tagcaaaagc tctttcaaat
    17461 aaccctccaa tattgctatt agatgaggtt acaacaggtt tagatgtttc tgtgcaagcg
    17521 gaagtattgg atttaataag agaaatccaa agagaattta agatttctat gatagttgta
    17581 tcccatgact ttgatgttat aaagatgctt gctgatagga caattgtaat gaaaaatgga
    17641 agtgtaattg agtctggtct tacagaccaa ataatggagg acccacaaca cccatatact
    17701 cagcaactta taaattcatt gctataaata tcgtttgtaa ataagcaaaa tgggggtttt
    17761 taataaaatg aaagaaacta tacttgaagt aaataatttt agtaaagact ttgagttaca
    17821 tgcattgaac aagactataa cggcatgtag taatataagt tttgaagttt caaaaggtga
    17881 gtttttagga ataataggta aatctggagc aggtaagtct accatattaa aaagtatcta
    17941 taaaacatat ataccaacaa ctggagaaat aatttttaat tcagaaatat atggtaatgt
    18001 agacctggct aaaattggag accgagaaat gataaatctt agaaagaaag aaataggata
    18061 tgtatcacag tttttaaaga cacttccacg aataactgca attgaactgg tagtacattc
    18121 tttaattgag agtgggtttg aaaaggattg ttcgtatgat atggcaaaag atatacttaa
    18181 ccaatttgaa ataaaggaaa atttatggga tgcgtatcca aataattttt ctggtggaga
    18241 aaaattaaga ttaaatttag ctcaagctat ggttaaaaag cctagacttt tgttacttga
    18301 tgaaccaact gcatcacttg ataatcaatc taaaatatat gtaaaagaaa aaatgttgga
    18361 gcttaaaagt caaggaacta caatgatagg aatttttcat gatatagagt ttatggaaac
    18421 tgttatagat aagaccttta ctatgacaaa agggactata agtgaaagtg gggtagccta
    18481 gatgataaaa ctaattcata atgcaaatat tgtattagaa gatatgattt tagaaaatgg
    18541 tcatttaatc atagagggag atacaataaa aaaagtatca aatgataaga ttgattacgc
    18601 atttgacttt aatgagatag atgaaataat agatgcaaaa aacttatatg taatacctgg
    18661 tattatagat attcacagtg atgccattga aaaggaaata gagccgagac caagtacatt
    18721 attaccattt aatatggcat tttatgaact agataaaaaa ctacctgcaa atggaataac
    18781 tacagtttat cattctatat ctttagggga tggagtagga gttagaagta ttgataactc
    18841 tttaaagatg attaaaaata ttgattcata taaaaatata gatagtaaga gcataaatca
    18901 taaagttcat ttaagatatg aagtgttgta ttatgaaggt ttagaaaagg ttttagaact
    18961 attagatgaa aataaaatag actacttatc aataatggac cattctccag gacaaggtca
    19021 gtatacaaat ccaacatttt ataaagagta tgctactaaa gtgtggggag ttacagaaaa
    19081 ttatgtagat acatggttag atgatttagt aaatctgcat gataatttag attggaataa
    19141 aatagctaat attataggta tagccaaaac taaaaatata aatgttgcat cacatgatga
    19201 tgatacatta gaaaaaatgg atttcataaa aaatataggc ataaatgtat ctgaatttcc
    19261 tataactctt gaagttgcaa aacattcaaa gaaattgggt gttctaactt gtttaggtgc
    19321 accgaatatt gtaagaggta aatctcataa taacaattta aaagcaatgg atgctatatt
    19381 ggaagattgt tgtgacattg tgtgttcgga ttatcttcca tcagctatga taaagtctat
    19441 gtgtatagtt gcagaaagaa taaataattt aaataaagca gtcagtttat ttacctcaaa
    19501 tcctgctaaa gcagttggta tatatgatga aagaggtagt ataaaagaaa ataaaaaagc
    19561 agatttagtt ttagtagata ttgatagtga atatccaaag gttgtaaata ctattgtaaa
    19621 tggaaaaaca gtttataaaa gggaggtata aagattgcta acagagttac attgtcatac
    19681 aagtatatca gataataatt ttactactga agaaataata aaaaaagcag cagaaaagaa
    19741 tataaatatc ttagcaataa caaatcatga cacattatct ggattggatg aagctcttgt
    19801 gcttggagag cattatggaa taacaataat accaggtata gaaatttcag cctatgatta
    19861 taaaaataga aaaagagttc atattcttgg atataacata gatttaaaca gtcaagaaat
    19921 aaaaaatttg tgcaatccaa tggtatgtga cagacataaa gcatctgtta agatggtaaa
    19981 taaaattata gatttaggat acaaaatatc tttggaagat gtaaagaaat attcatcaaa
    20041 gactggaata tttaaacaac atataatgaa agctcttata gataaaggat atacagataa
    20101 tatatattca agtctatata aagaattgtt tcatagagga aacggcaagg tatatgtatc
    20161 tttaaaatat gtggattaca gagatgcaat aaaagctata aaaaattctg gtggaatttg
    20221 tgtgttagct cacccgggtc aaatggataa ttttagcgct attgaagaga tggtaaaagt
    20281 aggattagat ggaatagaag tatatcatcc atctcacaac aaagaactag aaaaagagtc
    20341 gctttcttat gctaagaagt ataatctagt ggtcacagga ggctcagatt accacggatt
    20401 ttattctcaa cataattatg aacttggaag caagagctta aataaagaag atttatttaa
    20461 gtttaggtta gctttaggag gggtattata aatgtctttt ttacaagtaa aaaatgtagg
    20521 taaaagttat ggacaagtta aggtgttaaa agacatatct attgatattg aaaaaggaga
    20581 gtttatttgt ttactaggtc caagtggttg tggaaaaagt acactgctta gaataattgc
    20641 agggcttgag gataaacatg gtggaaaaat aataataaat gataaggata tgactaattc
    20701 accacctgaa agtagaaatt ttggtatagt ttttcaatca tatgcattat ttccaaatat
    20761 gaatgtttac aaaaacatag cctttggact agaaaataaa aacataagta aatctaatat
    20821 tgataaaaaa gttaaagaag ttctagaagt tgtagaatta agtggatatg agaaaaaata
    20881 tccatcacaa ttatcaggtg gacaacaaca aagggttgca ttagctcgtg cgattgcttt
    20941 agagccagac tttttacttc ttgatgaacc tttatctgca ttagatgcaa aagtaagact
    21001 taaacttagg gagcagatta gaagtttgca tagaaaacta ggcattacaa ctataatggt
    21061 aacgcatgac caagaagaag ctctttgttt agctgataag atggttgtta tgaatagagg
    21121 agaaataata caagttggaa cgccaaagga ggtatataaa aatccagaaa caccttttgt
    21181 agctgatttt ataggaacaa taaattttat agatgatggt attaataaga tagctattag
    21241 accagaagat ataaaagtag agtctaatag agattctaaa gataaggata taaaagtggg
    21301 agaaatttta gatatcgaat ttagaggatt taactataga attacagttg agtatcgttc
    21361 taagcagatg aaattagatg tagtttccaa agttgcagaa caaatgaaac tgtgtatagg
    21421 ttctaaaata aactttaaga tacctaaaga aggaattgta caatataaat cagagggatg
    21481 tgcttaatag ctaggggtga ataaatgata acatcaaaaa ataaaagaag aataagtgat
    21541 agcacaatac aaaaatattt cttgattttt tcggtaattg tactaatagt atttatatta
    21601 tttccattag tatcattatt aaaaaatgca tttatggata caagtggaaa ttttatagga
    21661 atttcaaact ttagtaaata tgttgaaaat ccaagtctta tagcatcatt taaaaattca
    21721 atttttgtat ctacagtctc atctttaata tcactatgtt tagcatttat atattcatac
    21781 gcaataacaa gaacaaacat aaagtttaaa gaatttttta aaactatggg aatgtttcca
    21841 ctttttgcac caactatgtt atatggtatt tcacttatat acctttttgg aaataaggga
    21901 atctttacaa ccatgggtct ggaagtacca ctatatggtc cattaggaat aattatttct
    21961 cagattctat ttacatttcc acaagctttt ttaatattat ctgtagcact ttcgatggct
    22021 gattacagac tttatgaagc agcagatagc ttgggtgcta gtgaatttaa gaaattttgc
    22081 aaaataacaa ttcctggtat aaagtactca ttacttagtt cattctttgt atcctttata
    22141 cttgcattta cagattttgg agcagctaag gtagttggag gaaactacaa tgtactggct
    22201 actgacatat ataaacaagt agttggacaa tttaacatac caatgggagc tactgtttct
    22261 atggtaatgt taattcctgt actaatagca tttacattag ataagatggc aagtaagaaa
    22321 caaggtatga ccataactgc aaagtctatg ccatataaga ttaaggaaaa taagtttaga
    22381 gatagagtat ttttaatagt atgtagtttt ataacattgc taatttttat tttaatattc
    22441 gtttcagtta taggttcagt tataaagtta tggccatata atttaagctt tacaatggaa
    22501 cactatacat ttaaaggtat tttgggaagt ggaataaaaa catatttaag cagtttgaaa
    22561 atatcaactt taacagcaat atttggaact atatttgtat tttttagtgc ttatatgata
    22621 gaaaaggtgg ataagtttcc taaattaaga caaacagctt accttttatc aatgatacca
    22681 atggccttac ctggcttagt tttaggtata tcatatataa ctttttttaa ctcagcaagt
    22741 aatccattaa attttttata tggaagtaca gcaatattag ttatagtaaa tgtagttcat
    22801 ttttattcag tttgttttat aacctcaaat tcatctttaa aaatgttgga taaagagtat
    22861 gaattagttg caaaatccat aaatattcca ttctataaag tattttttaa tataacagtt
    22921 ccaatgtcta taacatcaat acttgaaata gtagtatatt attttgtaaa ttcaatggtt
    22981 actgtttcag cattaatatt tttatataca cctcaaactc agactgcatc aatatcaata
    23041 ctaaagttag atgagatagg atatatagga ccatcagctg ctatggcagt attagtgctt
    23101 ttaacaaata taatagtaag acttttgtac gaatttgtaa caaagaaatt aaaaaataac
    23161 acacaaaaat ggcagcaaaa agatgttgca taggggaggt aaaaatgaaa tttaagaagc
    23221 tagtttcttt aggagtatta tcaacacttt tattaagtgt aataacagga tgtagtactg
    23281 attcatcagg ttcagattca aagtctgata gtaagacaaa aggggattta acagtttaca
    23341 cagcaattga agaagatagt atagagccat atttagctac atttaaagag aaatatccag
    23401 atattaaatt aaatattgtt agggcatcaa caggagatat aacagcaaga ttattagcag
    23461 aaaaggataa tcctcaagca gatgttgtat ggggagttgc agccacaagc ttattggttg
    23521 cagatgacca ggggatgtta gaaccatatg ctccaaaagg ttctgaagaa atagagtctg
    23581 catttaaaga tgataaagaa gttccttctt gggttgggat agatgcttgg atgacaggta
    23641 taacagtaaa cactaaagaa ttaagtgata agaacatccc agttcctagt tcttacgaag
    23701 atttaataaa gccagaatat aaaggtctta tatctatgcc taatccatct tcttctggta
    23761 cagggtactt aacagtatca gcccttatac aaattatggg ggaagaaaaa gcatggcagt
    23821 atatggataa gttacatgag aatattggtg tatacactca atctggttca gctccagcta
    23881 catcagcagc ttcaggagaa tatcctattg gtatatcttt tggatataga ggtataaagt
    23941 taaaagagga aggctatcca gtagaagtag tatttccaaa agaaggttct ggatgggata
    24001 tagaagcaaa tgctcttgtg aaaaaagata atataaaaga agcatcaaaa gtatttttag
    24061 attgggctat aagtaaagat gcaatgaacg aatactctaa aaactatgca gttactacta
    24121 tttctacagg aaatccaata ccagaaggat tccctaaaaa gccattagag caaatgatag
    24181 ataatgattt aaaatcagca gcaaaaaata gagaagatat actaaataaa tggatatcaa
    24241 aatatgatgg taagacagaa aaagagagtt aagtaaaact atacaaatgg agtaaaaaga
    24301 aaaatttttt ttaagtagat tttattatta taatataagt tgagaataat ataggtcaag
    24361 aaaagtgaat attctataaa aatgtataga gtaagtatac cattcagttt gaatggtata
    24421 ttttttattt ttagagatta atttagaaat agatatacta cagtttaaaa tagtatattt
    24481 taatttcatt attaaagtat agatgttata tataaagtaa ataagatatt aaataattat
    24541 aaactaaagt aaataaaata ttgagcgatt attaaaaaga tatatatata atttaatttg
    24601 agcagtttgt tatatattat aaaaaaagag aaaaataaaa ggtaaattgt ttctggataa
    24661 cttaaaattg gaagtaagaa tatttaacta gagaagaatt atttaagttt aggttagctc
    24721 taggagtagc tgattttata ggaacaatag attttatgga tgatgatata aacatggtag
    24781 caattagacc agaagatata agggtagaat ttaatagaga tttcaaagaa attatagacc
    24841 tcaaactcag accgtatcga tataaaaact aaagttagat gatatagtgt tgtagatagg
    24901 accatcagct gttatggcag tattagtgct tttaacaaat ataatagtaa aacttttgta
    24961 tgaattgata ataaaaaaat taaaaaccaa gacataaaaa tggcaacaaa aagaagttga
    25021 ataagggagg taaaaatgaa atttaagaag ttagtctctt tgggaatatt atcaacactt
    25081 ttattgagtg taataactgg atgtagtaat gtttcatcag gttcaaactt aaagtctaat
    25141 aataagacaa aaggagagtt aacagtttat acagcaattg aggaagatag tatagataca
    25201 tacttagcta catttaaaga gaaatatcca gatattaaat tgaatgtact tagattatca
    25261 gtaggaggta taacagcaag attattagca gaaaaagata atcctaaagc agatgtgtta
    25321 tggggtgttg cagctacaag tttgataatt ttagatgaca aagaaatgct agagccatac
    25381 gctccgaaag gctttgataa ggtaaattct gcatttaaag atgataagaa agttccttct
    25441 tgggttggaa tagatgcttg gatgactggt ataacagtaa atactaaaga attaaaagct
    25501 aaaaatatat cagctcctag ttcttatgaa gatttaataa agccagaata taaagatctt
    25561 atatctatgc ctagtccagc ttcttcagga acaggatatt taacagtatc agaactcata
    25621 caaattatgg gtgaagaaaa agcatggcag tatatggata aattgcataa aaatattggt
    25681 gtatacactc agtctggttc agctccagct atattagcag cttcaggaga atatcccatt
    25741 ggtatatctt ttggaaatag aggtataaag ttaaaaggtg aaggttatcc agtagaagta
    25801 gtatttccaa aagaaggttc tggatgggag atagaatcaa atgcacttgt gaaaaaagat
    25861 aatataaaag aagcatcaaa agtattttta gattgggcta taagtaaaga tgcaatgaat
    25921 gagtattcta agaattttgc agttactaca attgacacgg gaaatccggt gccagaagga
    25981 tttccaaaga atccattaga acagatgata gataatgatt taaaatcagc agcaagaaat
    26041 agagaggcta tactaaataa atggatagaa aaatatgatg gaaagacaga aaaagagagc
    26101 taagccttgc aataaaattt agtacaaaaa gcgcctagaa tataagtttt atatctaggc
    26161 tcttttgata tatttattta aaatgcatat ttctatataa aaataaatag tttagtacct
    26221 tgattaaatt aaataaaaac cttaaaataa agagatgtaa agaaaaacta aaaacgaata
    26281 aaaggtgaat ttatatgata aaagaaataa tagtagtaga gggtagagat gacgttacag
    26341 cagttaagag agcaatagat gcagaactta taactacagg tggatttgga tttccaaaag
    26401 gtgtaatgga gcgtataaaa gctgctaatg aaagacgtgg tgttataata tttacagacc
    26461 cagattttgc aggtgaaaaa ataagaaaaa aaatagcatc agaagttcca ggatgtaaac
    26521 atgcatttct accgagagag gaagctaaaa aggatggaga tataggtatt gagaatgcta
    26581 ctcctcaaag tataatagca gctctgaata aagttagaac tgaaaataca gaaaaaagag
    26641 atgaatttaa gcaagttgac ttaattagaa atggtcttat aggtaatgaa gaagcatcac
    26701 ttagaagaga tgcacttgga aaaatacttg gaattggata tggaaatgca aaacaatttt
    26761 taaatagatt gaataattat ggagttgaaa gagaagaatt tatagatgca ttaaaacaaa
    26821 tctaaacgta atgtttaatg tatgaaaaat atgtatataa aataaattgt ggaattacgg
    26881 ttaatttaag atgaattatt tataatatac accttatttt aaatcaatgt aaatacatat
    26941 aaataagtat aattaataaa attaaatatc aaaataagtt aaaaaggagt aaataatgga
    27001 tagactatct tcacataatg caacaaagga agtagtacaa aaatataatt ttaaattttc
    27061 aaagtcttta ggtcaaaatt tcttgataga tagtaatata attgataaaa tcttatctgg
    27121 agctagaatc acaagaggag ataatatcat agaagtagga cctggtatag gaactcttac
    27181 tcgtgagatg ggtaaaatag cagaaaaagt agtggctata gaaatcgata gaaatttaat
    27241 tcctatattg aaagatacct tgtcagattt agataataca gaggttgtaa atcaagatat
    27301 actaaaagta gacatacagg aattagtaaa agataaatta aatggtgggc cagttaagct
    27361 tgtagcaaat cttccatatt atataactac tccaatagtt atgaaatttt tagaggaaga
    27421 catacctgta acagacatag tagtcatggt tcaaaaagaa gttgcagata gaatgaatgc
    27481 tattccgggt actaaagatt atggggcatt atctatagct gttcaatact attgtgatac
    27541 agagattgtg gcaaaagctc caagacatat gtttatacca caaccaaatg tagattccac
    27601 agttataggt ctacatgtaa gagataaaag aaaatatgat gttcataatg aagacatatt
    27661 ttttaaaaca gtaaaagcat cctttggaca aagaagaaaa actcttctta attcgttggg
    27721 aggtcttggt tttcttaata aagatgaaat aagagaagta ttaaaagaag ctaatataga
    27781 cgaaaaaaga aggggtgaaa ctcttagtat agaagagttt tcagttcttt caaatataat
    27841 aaatacaaaa gtatcttcta aatagaaggt acttttttta attttgttaa catatattgt
    27901 ttagagaaac tgtgttaagg ggggattgca gtgggttcta aaagaaagtt taaaagagac
    27961 tatataatac tagaagctaa agacataaac tttagataca aagaacgagt actgccaaaa
    28021 gcttttgcaa aagttgaagt aaatgatgaa aaagcaatta ttgcattata tgtagaaaat
    28081 ctaaaatatg ttaaagatgg gtataaagca gtagcaataa aagatgacca tggattaata
    28141 gacttaggaa atataatttt aaatgaacaa ggaagaggtg aatttgtttt agatttagaa
    28201 gattatgatg atgagataaa aggtattgca ttattgtatg aaagaaatat acctttaatt
    28261 ggatttaaag gaaataagct tgaaaattat gaggacatat tatttcaaga ggtagaggaa
    28321 gaagaatatg aagaatatga agagttggaa gaagaagacg aagagataga ggagaatgag
    28381 gaaagtgata gagacgaaga tagtgaagaa gaatatatta tagaagaata tgatgaagat
    28441 gagtatgaag actattggga agatgatgaa gatattgaag aaggagagtt aaaagcaaga
    28501 acggaagtag aagattctga tgatgaggaa atagaagagt tcaataatta taaggaaata
    28561 gaaaattata atagggttaa agtacaggaa gaatatatag acatagaata tgatgaagaa
    28621 gaagaggaag aagaagtttt tgaagtaaat agagaagaag ataggtttgt ttcctcaaaa
    28681 cctactaaat caaagaaagt tcaaaaagat cagaataata ttgaaaaaat aaaaagagct
    28741 aatggagtaa gtcaaggaga gagtggaaat aaacaaacca gtacattact tatgccaaga
    28801 caaataaaaa aaggtttgaa attatttaaa gaagtaaaac cttttgtaac agattatata
    28861 gataagacaa gatggtggaa aatagagata aatcctacaa cactgtgtgg atatacaatg
    28921 ccttatttag gatatttaaa cgtattgaat tacactatgt atagtgatgc agttttacat
    28981 tcatataaat acagacacta tttatttgga gttcagtatg atgaatataa taagagaaaa
    29041 cattatattt atgctatccc aggaaataga aatgaacagc cagacaatgg aagcacagga
    29101 tttttaagat atcaagcttg tgataataga acaaatagtt taggatattg gctatgtttt
    29161 atagattcta gacaaagaaa tatagttagg taaaaataat aaaaatgaaa aaatttagac
    29221 tcaattctta ttttactgaa aaaattaatg aaatgtagta caaaacttta aaaactattg
    29281 ataatattta aatattacac tacaatatat cttgaaatac ctattatatt atggaggaaa
    29341 attatgataa acgagcaaag aattttaaat gagtttcttg aattagtaca gatagatagt
    29401 ttatctttaa aagaggggaa tgttgctaaa gtattagtta aaaaattaga agaaataggt
    29461 tgttcagtag taatagacaa tgctggtgaa aaagcaaatg gagaaactgg taatataata
    29521 gctacattaa aaggaaataa agaaggaaag aaaatattat ttagttcaca tatggataca
    29581 gtaactccag gtataggggt aaaacctata gtggatgaag caaatggaat aataaaaagt
    29641 gatggtacta caatattagg ttctgatgat aaagcaggta tagcagctat attagaaggc
    29701 ttaagatata taaatgaaaa taacatagaa catactgata tacaagttgt attctctata
    29761 tgtgaggaat gtggattagt tggagctaaa aatttagact atggaaaaat agattctgaa
    29821 tatgcattta tattagatag tggtggttct ccaggtgaaa taatagtaaa agctcctgca
    29881 caagatgtaa taaatgttaa gatattagga aagactgctc atgcaggtct tgaacctgaa
    29941 gctggaataa gtgctataat ggtagctgcc agagcaatag aaaatatgaa tttacttaga
    30001 atagacgaag aaacaactgc taacatagga attataaacg gtggaacagc tacaaatata
    30061 gtaacaggag aagttaatat agttgcagaa gccagaagtc taaaagaaaa taaattagat
    30121 gttcaaacta aacacatggt agagactttt gaaaaagctg cgaaagattt tggagctcaa
    30181 attgagatag atgttaatag agcatacaca ccaatagatg ttagtgagga ttctgaaata
    30241 ataaaacttg caaagaaggc tttttcaaac ttaggtatag aaggtcatac tgaatctaca
    30301 ggaggaggta gtgatacaaa tatattaagt aagaatggta tcgaagctat aactttagga
    30361 attggtatga aaaatgctca tacactaagt gaacatatag ctataaaaga cttatatgat
    30421 tctgctaaaa tggtagtgga gataataaag gaagcataaa tttaaataat agtgtataat
    30481 atatatatcg cctagcttaa gctaggtgat ttttcatttt atagaagatt gataatctct
    30541 attgaaagca aagagtatac atttgcatat aagagagtgt ttaatttatg ttcaaatgta
    30601 ggttcttaat aatatttaga ttgtttcata agggaggaat aaatatggaa acaaggtatg
    30661 aagaagcaaa taaaataaca atacaatcaa tactttggaa tatagtatta acaataatta
    30721 aggttatagc tggtgttatt ggtaattcta gcgctatgat tgcggatgga ttacattcag
    30781 catcagatat tataagttct ataggagtac taataggcaa ttatgtatct tctagacctg
    30841 gagatagaga gcataattat ggtcatgaga aagcagagac attagtatca tttgtgttat
    30901 caatattgct tattttcgtt tctataacaa taggtataga ggcaattaaa tctttattta
    30961 acttagatgc attaagtgtt ccatcaatat taccattagt agtatcagtt atttcaatcc
    31021 tgataaaaga gtatcaatat agaataacaa taaaagttgc taaaaagata aattcaccag
    31081 ccctaaaagc tgatgcttgg catcacaggt cagatgcttt atcttcagta gctgctttta
    31141 taggtatagg tggttctatt ttagggttta aaccattaga cccaattgcg tcagttgtag
    31201 tggctatatt tgtagctaaa gtaggaatta gtatacttat aagttctgtt aatgaattga
    31261 tggatgtttc agttgatgaa gaagagataa aagaattaaa atttattgta gcagatacag
    31321 aaggtgttaa aaatcttggt gatataaaga ctagaaaaca tggagctatg gcatatgtag
    31381 atttgactat atgtgtggat gaaaatttga cagttaagca aggtcatgat attgctacca
    31441 agcttgaaaa acatataata aaacatatgg aatttgtaaa aggtataaca gttcacgttg
    31501 aaccatgtac taattgccaa ggaaataagt gtaataatta attaaaactt aaaaaaaagt
    31561 gtaaaaagtt atataattgt tttatgatta taaactctag gggtggttaa atgagcaaga
    31621 aaagaaaact aaagaaaaaa gctgcaatat taataggagt agttgttttc tttataatat
    31681 actttatatc ctttagaata ggaataaata tggaaaagga taaaagtgaa gtgccaaaga
    31741 aggtagaaaa agaagaagaa gataataggt ctttaaggac tcaaataaaa gagaagaaga
    31801 aggtttatat atcagataaa aatatagaaa acataaaaat tgaagaagat atgtgggatg
    31861 aaataaagtt atttttctca gattttaaga aagtaagaaa tactacttct tcttacgaag
    31921 cagtttatga aggttattct gatgatggag ttaaattttc tactgacctt aactttttta
    31981 gagtatatac tgtaaataaa gaagaatatt ataaagtacc agttagttct aaggatgctt
    32041 ttaagaaact tttagatgag agtatatata cttcgtttga ccttttaagt cattataaag
    32101 attggaaaga ggttactata actcatggaa aagaaattaa aaaagttcat agatggaaat
    32161 ttgatgattt agcacataag atgatttcaa agagggttgt aggaaaagtt caacctgaga
    32221 aaagtaagga aagaagtgac tataatttta ctataaatat aaaaggtgat aattttgatg
    32281 taaaagttga tacaatggga aaagattatg taaaagttac ttctaagaaa aatgtatctt
    32341 actatgaggt tcatacaagt ttatttgatt accttaaaaa tgagatattt gaaattggaa
    32401 agaaataaga ctttaagaag gctatcaaat tagatatttg atagcctaaa ttattttata
    32461 tattattttt caaccaacaa gtctgcaacc ttataaattg gaccagcacc agcagttatt
    32521 actaaatcat tatccttaac attttcacgt aagtatttta ctatatcttc aaattctttt
    32581 atatagatgg catctacatt gttttgatat aatttctcaa ccaagtcctt agaatgtata
    32641 tctcctgggt ctttttccct agcagcatat atatctgtta tgataacttt atcagcagca
    32701 tagaatgcct ctgaaaattc acctaaaaga gattttgttc tagtataagt atgtggctgg
    32761 aagatacacc ataaagttga tttttttaat tttttggcag cagctaaagt tgcttttaac
    32821 tcagttggat gatgtgcata atcatctact actaaagcat tcttatagta ccctttagtt
    32881 tcaaatcttc ttccgacacc tttataaatt tttatatttt ttcttatagt ctctaagtca
    32941 ataccagaaa caagagctgc cattatggca gaagaagcat tgtatatgtt atgaagacca
    33001 tatacagaaa gttcaaattc tcctaaatct cttccttcat attctattct aaatattcca
    33061 tgaccactat tgtcaaagtg aatatctttt attacagcat cattgtcaga atctctgccg
    33121 tattttataa tagtagcttt aacatcatac aatatgtcat caatattttc atcatcgcca
    33181 tttattatga aatagccttc tgggggtaat aattttccaa atttgttaaa agaagccttg
    33241 atttcatcta ttccagagaa ataatcaaga tggtcttctt caacatttaa aactatagat
    33301 atctttgggt taaaattaag gaaactatct acatattcac atgcttcagt tataaagtgg
    33361 ttggaattac ctatcttaac attaccacct atcatactta agttaccacc aactagtata
    33421 gttgggtcta agtctgcata ttcaaagatt gtagataaca tagatgttgt agaagttttt
    33481 ccatgtgttc cagaaactgc tatagaattt ttatattccc tcatgatttg gcctaaaaaa
    33541 gttgctctgt tcattaccaa tttatttttt tctttagcag ccacaagttc ttcattgtct
    33601 ggatgaactg ctgctgtata aacaaccata tttacatcat ctgatatatt atcttttttc
    33661 tgaccaatat aaatagttgc cccttggtcc ctaagtttgt ctaataggta tgattcgttt
    33721 gaatccgaac ctgaaacttg ataaccttta ttgatacata tttcagctaa tgcactcatg
    33781 ctgataccgc caattcctat aaagtgtata ttcataataa aaccaccttt ctttttaatt
    33841 aagtcctaca atatgaacta tttctgtggt ataataatat aatgttcaat attttataat
    33901 taatatgcat ataatattat atgtatttta tttttgaata taataaaaca taagtaatat
    33961 tataccacaa taagaattgt tatgcattag ttagtttaac gttatataaa ggtacttgta
    34021 ggaggaaata ataatgaaat ttaaaagaac agagagaatt ggagcaatag ttaaaatact
    34081 gtctgataat ccaaataaaa tatatacttt aagttatttt acaaatcaat ttaatgcagc
    34141 aaaatcaaca ataagtgaag atttattagt tgtaaaaaat gtatttgaga agctacattt
    34201 aggtaaagtt ataactattt caggagcagc aggaggagta aaatatatac ctaaaacttc
    34261 tatagcagaa aatgcagagt tcttaatgga attatgcgag agaatatgtg acaagtctag
    34321 aatactatct ggtggttttt tatacttgat tgacctaatt tacgacccta caatagctgc
    34381 taagatagga aagatattcg catcaaatat tgagtatgta gatgcagatt atgttgtgac
    34441 aatggaaaca aaaggtatac ctatggcact tatgactgca aaagctatga atttacctct
    34501 agttataata agaaaagata taaaggtatc agaaggtcct acacttagca tgacatatgt
    34561 gagtggaaac agttctaaag ttgaaagtat gagtttgcca agaaaagctc ttaaaccaga
    34621 tagtaaagtt ataattatag atgatttcat gaggggtgga ggtactataa aaggaatggt
    34681 agaccttatg aatgaatttg gtgcagaggt tataggtact ggagtattca tatctacaac
    34741 gaatccttca gaaaaaatgg taaaagatta tatttcattg attcaattgg atgttaatgg
    34801 agataaaata gtagtagaac caaacttaaa gacttttaaa gatgaatata gaaatgaaga
    34861 tttagacgaa gaagaagatt cagaatttga atttgagata gatgaagatt aataaaaaaa
    34921 atatttagaa aaatatgtaa aaataaaaag aggatatccc tagttgttca tagaattatt
    34981 taaaaaacag tttatattaa tttattccag cgataataat tttaatataa attaatttct
    35041 gtcaaaatca aagggggata ttttggtatg aagataactg acgtaagagt aagaaaatta
    35101 acagaagaag gtaagatgaa atgtatagtt tcaataactt tcgataattt gtttgtagtg
    35161 catgatataa aagttataga aggccataat gggctattta tagcaatgcc tagcagaaaa
    35221 gtaggcgaag gaaatttcag agatatagct catccaataa atgcagaaat gagacaagtt
    35281 ttagaagatg cagttttaca agcgtatcat gaagcattag ttcaatggga agtagctgct
    35341 gaataataat aaatatataa aatggagaga gcccgaacat ttaggctctt tttaaatgta
    35401 gaatttctat taaaatatgt actttttagt aaaaaagtgc tatattatta gtgataatgt
    35461 atatgttgta tacaaattgt tagataaatg tattataatg tatattgatt aagagaataa
    35521 tacgaataaa attaaattag attgaatgga gtgtatcaat gaactttaaa gctataatcc
    35581 ttgctgctgg caaaggaaca agaatgaagt ctaaataccc aaaagttata cataaagtat
    35641 gtggaaaaga aatggttaac catattatag atgtttcaaa aaaatcaggg gtaaaagata
    35701 ctgtcgtgat tttaggacat gaagctgatg tagttaaaga aaagcttgca gaagaaatta
    35761 taatagccat gcaaacagaa caacttggga caggacatgc tgtaaagatg gctaaagaat
    35821 atattaatga tgaagataca atagttgtac tttgtggaga tactccactt ataaaagaag
    35881 agacactaaa gagactattt gaatatcata tagaaaataa atatcatgct acagttctta
    35941 caacaagagt tggaaatcca acaggttatg gtagaataat aagagataag aagggtgacc
    36001 ttcttaaaat agtggaacaa aaagatgcaa attcagaaga aaagatgata agtgagataa
    36061 actctggaat ttattgtttt aatggaaaga gtcttagaga agcactagat ttgctaaata
    36121 ataacaattc acaaggtgaa tattatttaa ctgatactgc taaaataatg agagataaag
    36181 gattaaaagt tggtgcattt gcaggttcaa ctattgaaga acttatgggc gttaattcaa
    36241 gagtagaatt atcaaaggca gaagaaatta tgagacgaag aataaatgaa tctcatatgg
    36301 taaatggagt aactataata gacactaact ctacctatat agaatctgat gttatgatag
    36361 gcaatgatac tataatatat ccaggtgtta tgttacaagg aaaaacaagg ataggttcag
    36421 attgtataat tggaatgaat tcatctataa caaattcaga aataggtgat ggtacagaaa
    36481 taaaaaattc aactataatt gatagtaagg ttggagaaaa ttctactgtt gggccatatg
    36541 catacttaag accaaagagt gacttaggaa ataatgtaaa aataggcgat tttgtagaag
    36601 taaaaaatgc tattatagaa gatggctcaa aagcatctca cctttcttat ataggagatg
    36661 cacatgtcgg taaaaatgta aatattggat gtggagtagt atttgtaaac tatgatggta
    36721 aaaataaatt taaatcaata gtaaaagaca atgcgtttat aggttcaaat tctaatttag
    36781 ttgcaccagt agttgtagaa gaaaaaggat atatagcaac aggttcaaca ataacacatg
    36841 atgtcccaga tggtgctttg gcaatagcaa gagaaagaca agttatcaaa gaaggatggg
    36901 tagaaaaaaa gaatcaaaaa gacgaccaaa gtaagtaatt atgtagttta ttattttggc
    36961 taaaaaattt agctcgaaat tattataaat tatattcatt agtattttaa aattttcagg
    37021 agggttacat aaatgaatac tagcggaagc gagattaaaa ttattgcagg taattcatcg
    37081 aaagagttag cacaaaaaat agctgactat ataggtgtat cagtactaga ttgtgaagta
    37141 ggtacattta gtgatggtga aatatgtgta aacatgaatg agacagtaag aggttgtgat
    37201 gtgtttgtag tgcaatctac taacagccca gtaaatgata acttaatgga gttattaatc
    37261 ttaattgatg cgttaaagag agcatcagca ggtagaataa cagcagttat tccttactat
    37321 ggatatgcaa gacaagacag aaaggctaag gcaagagatc caatcactgc taaattagtt
    37381 gcaaatttaa taactgctgc tggagcagat agagtattaa caatggattt acatgcagct
    37441 caaatacaag gatattttga tataccatta gaccacttac taggtggaac aatattagca
    37501 aattatttta atgaaaagaa aatagaagat ttagtagtag tatctcctga cttaggaagt
    37561 gttactagat caagaaaatt tgctaatact ttaaatggag aagttccaat agctataata
    37621 gataaaagaa gaccgaaagc taatgtatgt gaagttatga acttaatagg tgatgttaaa
    37681 ggtaaaaatg ttatactatt agacgatatg atagatacag caggaacaat agttaatgct
    37741 gcaaatgcac ttaaagagtt tggagcaaaa gatgtttatg cttgttgtac tcatggtgtg
    37801 ttatcaggtc cagctattga aagaattgct aattcagaaa taagtgagtt aatagttctt
    37861 gatactatac aacttcctga agagaaaaga atagataaga taaagataaa aacagtagct
    37921 cctttatttg gagatgctat aagaatgata ttctctaatg aatcagtaag taaattattc
    37981 taatttattg tatttatgag agttgtttaa aaggatataa accttttgag caactctttt
    38041 ttgattttat acttatggaa tgttattttt ttcttgttag aaaggtatat attattgtaa
    38101 aaagagatta tagtttaaaa ttatgtatat ttaagtaaaa taaacctaat aaagggtata
    38161 ttattcaaaa tattatgaat ataaaaaata ttacaaaaaa gtattgaaaa aattcagatt
    38221 atgtagtatt atgtaaatat aaataaagaa agaggtgttt ccaatgggaa ggttagaaga
    38281 tgaaccccta ataattaaat gattcagtca caatctaaag tgaatataaa aatctattga
    38341 aagtttattt ataagaattg aaaatagtat ctattctatc tttagtgttt gtgagagggt
    38401 tctggattat ataagattac tgtaaagagt aattttgcac aataagcata tctgtaaatg
    38461 aagagtttcg tttatataat gattgtggct gaatcataac tataatagat agaatgttta
    38521 taatagatga ttttataaaa tactatattg taatagcttt tagctttcaa ataaatatta
    38581 gcaaaggatg gaaatgacaa tgataaaact aaatgataat ttataaatat ctccttacga
    38641 tatataataa ttgtagggag atgtttcttt ttttgtatag cataattttt ctagaaattc
    38701 tgtttcaata tgtaaaaagt tggtatatat aaatagtata aacagtgttg ctaaaatatg
    38761 gtagatatta tataatataa gtggatatgt atatacatta tgagttatat aatttgaatt
    38821 taataaaata gaaacgttga tttatgttct gtaatgtggg caccttggag catattgagt
    38881 tagtggtgca accggctatg aaattgtatt tatttataaa tactattatt ttcatagctt
    38941 tttttattta aaaaatggtt atatgttaga aataaatttg gttagtacgt ttaaataatt
    39001 ttacttagat ttttaaccag atatattgta agtaagtatt aattaaaaaa tttaaggggg
    39061 aataaaaatg aagttaaaaa agaataaaaa aggtttcact ttagtggaat tattggtagt
    39121 aattgcaatt ataggtatat tagcagtagt ggcagttcca gctttattta gtaatataaa
    39181 caaggctaag gtagcaagtg ttgagtctga ttatagttca gttaagagtg cagcattatc
    39241 ttattattca gatactaata agataccagt tacaccagat ggtcaaactg gtttaagtgt
    39301 tttagagact tatatggagt ctctgcctga taaagctgat ataggtggaa aatataaatt
    39361 gattaaagtt ggtaataaat tggtattaca gataggtaca aatactgaag gagttacatt
    39421 aacagaggca caatcagcaa agttattgag tgatataggt gaaaataaaa tatatacaag
    39481 cgctacaaca aatagtttgg gaaatccatt aacaagtaat acaaaaatag ataataacgt
    39541 tctatatata gtacttatag ataatactgt gatggacaca acaaaatagt taaaacaata
    39601 tttttataaa aatattgtaa atttaatgaa aaatataata taaaagacag gaagaaaatc
    39661 ttcctgtttt tttatactat tttctttaat gttcaattat gaatgatgat ataattgaga
    39721 tagtaaagga gtgaaacctg tggctaaaaa agttagaata ggagacaaac tggtcgaaaa
    39781 aggatatata acagaagaac agcttaaatg ggcactatca gaacaaaaaa acagtggaaa
    39841 aagactagga gaatttctag tacaagaagg gcttatagat agtaacttac taataagtgt
    39901 attaaaagaa ctcttggata tagaaagtat atttttagaa gggacagaaa tagacacttt
    39961 agctacaaaa atggttccag aaaatatatg caaaagatat actgtatttc catttaaaat
    40021 tgatggaaat aaaatatgtc tagccatgtc agacccacaa gatagagaag cagttcaaga
    40081 tgtaaggcgt atgtctggta aagacgtaga gatatttata tcaagtacag aagatataaa
    40141 caaagcaata ggtcatgctt atgcacattc agaaataaat aaggcaatga ctgagtacaa
    40201 caaaaataga actggtggag taagagaaac agtaatactt gaagaagatg taaatgctgc
    40261 tccaatagta cgattagtaa ataacatact agaaaatgct gttagaatgg aagcaagtga
    40321 tatacatata gaacaaagtg aaaattacat gcgtgttagg tttaggatag atggaatgtt
    40381 aagagaatat atgaggatga actcagcacc atataaggca gtaatcagcc gtatcaagat
    40441 aatgtcagat ataaatatat ctgaaaagag aataccacag gatggtagaa tctatttaaa
    40501 agtagataat aaacccatag attttagggt ttctacaatg ccaactaaca gggatgaaaa
    40561 gatagctatg agagttcttg ataagagtaa ttttatggtt agtaaagaag tactaggaat
    40621 agacgaacat ggctctaaga tatatgatga attgataaac actccttatg gtcttatatt
    40681 ggttgttggc cctactggaa gtggtaaaac cactacattg tattcaatgt taaatcaact
    40741 aaatacagaa aatagaaatc tattaacaat agaagaccct atagagtatg aattacctgg
    40801 tgtaaatcaa tctcaaataa atgaaaaggc agggcttact ttcgccagcg gacttagagc
    40861 ttttatgcgt caagacccag atataataat ggttggagag ataagagata ctgagacagc
    40921 agaaatagca attagagctt ctcttacagg acatttagtt ttaagtacat tacatgcaaa
    40981 tacagcagtt ggagcaattt cacgtctttt agatatggat gtggaaagtt ttttaataac
    41041 ttcatctgtt ttaggtgtaa tatctcaaag acttacacga aaaatatgtg agcattgtaa
    41101 ggtttcatat gaagcagata ttggagaaaa aaaggctctt ggtattgatg taaatgaatc
    41161 agtaactatt tatagaggaa aaggttgtga aaggtgtaat aatactggat ataaaggacg
    41221 tctaggtata tttgaaatgc ttgaaattac tccagaaata aaagagttaa tagattcatc
    41281 tgcaaatcaa agagaaattt taaagatggc tagaaaacaa ggtatggtta gcttaaaaga
    41341 ggatatagtt aaaaaagttt taaatggaaa gacaacagta gaagaaatga ttagaataat
    41401 tttgatgact gattaaagga tgatattatg aaaagtttta aatacacagt aataaaagag
    41461 gacggaaaaa agcaaactga tgtgattgaa gccaacgatt ttaatgaagc tagaaatctt
    41521 cttagaaaga gaaatcttag agttattgaa atcaaagaaa gcaaaaataa aaatataaga
    41581 ttatctagta gaaagagaaa aaaagactta ggagctgacc agataagcca cttttgtaga
    41641 cagtttgcta ttatagtatc ttctggtata aatagtattt ctggattaga aacattagct
    41701 agaaggtcaa ctaacattac attaagagaa gaaattaatc gtatagtggc agaaataaaa
    41761 gtaggttcta ctattgcaga ttcaatgctt tcatcaaaat caaagtttcc aaagttactt
    41821 ggtgctatgg tagctacagg tgaagctaca ggtaaattag atgaagtatt aaaaagtatg
    41881 gcttcatttt atgcaagtga acacagggtt aaacaaaagc ttagaaatgc tgcaacatat
    41941 ccattaatag ttttgatttc ttcatttata atgattttta tatttacaac atttatggtg
    42001 cctaagatga ttaattcaat tacaacagta ggagcaactc ttccacttat aacaagaatt
    42061 gttatggggt ttggtatgtt tatgaagaag ttttggataa tagtactttt agttattata
    42121 ttatttatct atcagtttaa aaaatatcta aaaacgtcag ttggaagggc tcataaagat
    42181 agagttataa ataagatacc tgttttagga aaaggaataa attgtatggt agctacacga
    42241 ttttcaagag cattgtatct atttgtatca acaggatacc cattagttca agggcttgat
    42301 tatattatag atagtgtaaa taacaccatg gcagagaaga ttttagcatc agctaaagat
    42361 ggaataacaa gaggagaagg cttagcagaa aatttagaga gatatacgta ttttgactct
    42421 gtgttggtac agatgatagc tataggggag cagacagggg aattagagaa tataagtaga
    42481 cagatggcag aattttatga gtatgaatcc gagatttatt taaataggat ggcctcaatg
    42541 atagagcctg ttttaataat tgcagttggt attatagttg cgatactggt tgtatcaata
    42601 ttcatgccaa tgcttagtat atatgatgcc atgtaggagg gaaaggacaa atgaacaagg
    42661 tttatgtatc atattatggc gattctattt ccatagttga gggcagatat aaacacgata
    42721 aggataagtt ttatgtaaaa aattttaatg tgttttcaat agaggatatt gcacaagatt
    42781 tttctaagga aaagtatgct ctattgagat acgcattaga aaatgttaag ttaaaatcaa
    42841 gacatgtagt tttttgttta aatacaagag atgtaatttt aaaaccccaa aaacttccta
    42901 atatggacaa aaaggattta gacggattta tgaagctaga gatggacgaa atgatggctt
    42961 tggagtcaga tgaatatgta ttttcgtatg aagtatctag tgaaacacct gataattcag
    43021 atggcgatga atcaagttta aatcttatta ttggagctat acaaaaggaa gaggtaaatg
    43081 caattgtaga gataatgcat gaatttgatt tagtattaga tagaatagat acattatcaa
    43141 cagcttactt aagaatgctt aaaaatttag actacaatga tataatggta accaatataa
    43201 gtgacaatag cacaattata aatatatata agaaagatac tttatttatt agtgataata
    43261 tacctataaa gataactgct aaagatatgg acatgcaatt attaaatgta gtagaagaat
    43321 caaaaggtct tatgaattac tattcgtcaa gaaactttgg taaggttacg gatactatat
    43381 taatattagg aaaaagatat tccaataaat taatttatga aacttttagc tctgcatttt
    43441 caagctatgt ttctcaaggc cttactgaga taatggatat atctgatatg ataactggag
    43501 atatagatga agatgatata aattcaattg tagagctttt aggaagtatg atagaaccta
    43561 aagggaaaag agattttgaa aatataaact ttttaccaat ggatatactt agaaaacaga
    43621 taaaagctaa acaaatgaaa aacatactga aaattgctcc aatagttatt ttgattcttg
    43681 ctatacctta tttatgttta attttttcaa ataatttagc aaataaggaa cttgaaaatg
    43741 ttaatggtga aataagaaat attgaatctg catattatca gattggaaat atagaaaaaa
    43801 tgatacagag taaaagcgaa gaaataaagg tatatgatat gttaattggg aaaaaggtaa
    43861 agtgggaaaa tttattggat gatatagata aaaatactcc aagtaaagtt gaactaacaa
    43921 gtttatcaac aacttatcaa aatcttggaa ctaataataa agctgataat tcaaaaagta
    43981 ataagactac aacagataaa tcaagtaaca atgaaacaaa atcaagtacg aaaagtgata
    44041 gtaatggaga taaaacagat aatgatttaa atgaagataa gactacaaat gtgtcagaag
    44101 cattaactgg aaatagtgag actaatagta ataacacaga taaagatgat aaaactccat
    44161 tgtatgataa gataccaaat gttataaata tagaaggaaa tgctaaagat tcctcttatg
    44221 taggtcaatt tttatataaa ttaaagggat tatactattt caaggatgtt aaattacata
    44281 gcataaaaag taatgcagaa gattctaact atacatttag tatgacatta tacctgaaag
    44341 aaggtgtatt aactaatgaa taaagatata tttaaaaaag atataaaaga tatatttaaa
    44401 atggaagtta gtctaaaagg gattcttata aaggttataa ttttattagc tattttatca
    44461 tatggttatt ttggacttta ccctcaatat cagaaatata ttgctacaaa aaattctgta
    44521 aaacaggcta ctgaaaaatt aaatatatat aaagagaaaa ctgcacttat gccaaaatat
    44581 gaaaaacaac ttgaagaagt tgaaagtgaa ttaaaagaaa aaagcaaaaa tcttttatat
    44641 gatatgcaag atggtctttt tttaataggt ttagataaaa aaatgaaggc ttataatatt
    44701 gacttaacca gttatgacgt tggaaatgtt aaagaatacg aacattttta tggtatatca
    44761 acaacaataa gtgttagagg tgattataga aatgtcaaaa aattaatggc ttatttagaa
    44821 gaacaaaaaa atgttactca aatactagat tatacaatga cttcatatat agaaccagaa
    44881 aaagaagagg aagaaacaac aaacaataag gatttaatta aaaatgtgaa cataaataaa
    44941 gatacaaaaa tatactgggt ggaaggcgaa aataagtggc atttatataa ggattgtcca
    45001 ttgataaaag agggagagat tctaaaaaat gacaaacttt tagataggtc tactaattat
    45061 ctatgtaata cttgtgtact tagagctgca acagaaaaag aagcaaaaaa agaagtcgta
    45121 gctccaaagg cagaaggtgt agtagatgct acatttaaat ttataatgta cactaaagag
    45181 aatactacaa tgatacttga tacagataat ccatcttcat ggagaccagg aaaatataat
    45241 ccatttaagt ctagtgcgaa ataaagctaa gtaagcttta aagataggtg atgagtttgt
    45301 acaaaaataa tgaaaaaggt cttactttat tagaagtaat aatagctgtt tttatactga
    45361 caatagtttt aagtatttct tataaagtgt tcaatggtat aacatcagca gtaaaaaagc
    45421 aacagattat tacagatgct caagtaaata ttaatctaat taataagtat ctaaatagag
    45481 atttggaaaa ctgtaaagaa ctaactaaaa ctggttcagg taataattat gaatacaata
    45541 tagagatgcc agataatgtt gtaaaatatg aagtttctat agaaactaaa aaaaatacag
    45601 aggtatattc tgtaacgaga atccaaaaca atacaattga tacagaaaat gaagttagag
    45661 aagagataat ctataatcaa ccactagttc aaaacaataa agaaatgaag gaaacaccat
    45721 ttaaaataga aaagcaaact ggtaaatcta tttatactgt aagtatatac tataatgaat
    45781 cagtgcaaga aagtcataaa aatagcaatt taaataacaa aacttataca tttgatgtta
    45841 tgtcaagaat aggataataa gaatttatta aaaaataagt aggttgataa aatatgagta
    45901 aaaagaatag tgaaagagga ttttccttaa ttgaagtgtt agtagctatg gctattatgg
    45961 gaattgtact atttgcattt tttaatatta tcaatactaa taacaaagca aacactaaaa
    46021 atgacacaga tataacttcc ttaaactatg tccaaagtga aatagaaaat ctaagagaaa
    46081 agataaaaag tggagaattt gattttgata gtttagataa actagaagat ggaactgtcg
    46141 tatatgaaaa attaatagat aaatcaaaaa aagttgtata tgataaagtt cttagtgaag
    46201 gtgatgtgag cttatatgat actccatatg aaaagattac aacaataaaa gatgaagatg
    46261 gtaatttaat cgacaaagga aatattacaa ataagataaa gactattgta gaagataaaa
    46321 gtggtcagat atataaaata gctgtaactg ggaaaagcat gaatgactat tcaagtaaaa
    46381 aagaggttaa aattgtaact gaaatattta aagataaata attttacttg ggggtgagat
    46441 tttgaggaag tggaataaat ttaaaagtga aagaggggca gctttagttt tagttttggt
    46501 tgtagtagca ttgctttcta ttgttggatt gatattttca aatcagatag ctaatagaat
    46561 aaaatctaca aagactacta atgaaggtat acaagcaaag tatttagcag agacatgtgt
    46621 ggaaaatagt attgataaag catatgaaaa attgtatgat gagcttgaga aaatggataa
    46681 tgagtttaaa agtgaaaatc aagaaaaatc catatctagg tcaaaattaa gagatatatc
    46741 agatgaagat tttaataatc aagatgagga aaatattgaa gcagaaagac ttggatatat
    46801 gaataatata aatttctatt taaataaagc ttcttcagac ttggaaaaag ctagtatgga
    46861 attgaaaaag ctatatgatt tagatatgtt ggactataga gacattgaat atgtagatgc
    46921 aaatattatt tcacatagag attctatctt agaaatttgc aagaattata cttctggaga
    46981 tattagtaaa ataaatgaat atattctgaa agaagatata gactcaacta cattgattga
    47041 ggcaaagtta gtgaataatg atattctttt aaaaatgttt ttagaagaaa ataaaattga
    47101 aaatgaacat ttaaattcag ccttttcaca tacttataag gcattagata acatttcttt
    47161 ggcgatgcaa aatatgattg agtatagaca tacgtttcat atagatgagc caaaagtaga
    47221 agtatctaat gggattccag attctcaaca atattatgaa ctaatacaaa atcctataat
    47281 aaatagtatg gagtatatct ggaatagtaa atgggataca ttagaaaaac tattggaaat
    47341 tttaccaaat caaactcaag gatttaattc tttaagagtt catttgagaa ataatgtaag
    47401 aaaatttgag aagttaagcg ataatatatc tagtggtaaa aaaaatacag caaaaaattt
    47461 cttaaagtat aaagagttat tgtatgaaat atcagaccaa tgtaatcaac taaaaagtat
    47521 gtcttatgag aaaatacctg ttaaatatga taacatggct ttgataacaa cttttgacta
    47581 tatacaaaat gagcttttag cagagataaa atgcagatta aaagaattaa aaccacaaga
    47641 aattgataaa acagaaggta ttactataaa aatacctttt tataaggctg actacgacat
    47701 gacaaaagaa ggatggccta aattaaaaga aaatggttca ggggcggagt taagcttaat
    47761 ggtaactggt gataaagatg gaataaaaga agtagaagtt acagatggta aaaaaaatat
    47821 tattggttta ggtgttgaag aaaattcaaa ttcaaagtat aaggttgacg ccattgtaaa
    47881 ctttaatcta aatatagata caaatgtagt tggaaattat gatataaaag ataaaatttt
    47941 gattaatcat gatataagtt catacaaaaa agtaaactaa atataagaag caacttagta
    48001 taaataaata ataaaataat tttattattt actactaaaa aaataagtaa actaatagaa
    48061 gatatggaga gtaaatatta tgtatattta tgatttatta gagcaaggta taagactaaa
    48121 cgcatcagat atacacataa cagttggaac aaatcctgta gctagagtaa aaggtggatt
    48181 tgtaaaacta agtgaacaaa tattgacatc agaagtaaca atgcaaatgg caaaagacat
    48241 agcaggggaa tctatgttta aagtaataga agaacatgga gaagctgatt tttcagcttc
    48301 tttaaaaaca ggagaaagat ttagggtaaa tgcatatagg caaaaaggaa attacgcaat
    48361 agcaataagg actataacag cagaaattcc aacatttgaa aaattaggcc ttccagagag
    48421 tataaaatct tttactgaaa agcataaagg attagtttta gttacaggtc caacaggaag
    48481 tggtaaaagt acaacacttg caagtatgat aaatataata aatgaaaaac aacaaaaaca
    48541 tataattaca cttgaagacc cgatagaata tgtacatcat cataaacaaa gtttagtaaa
    48601 tcaaagagaa gtaggaactg atacagaaag ttttcattca gcccttagag ctattttgcg
    48661 tcaggaccca gatgtaatac taataggaga gatgagagac cctgaaactg tttcaatagc
    48721 attaacagca gcagaaacag gtcatttagt attttcaaca ctacatacag tgggggcagc
    48781 taagacaatt gatagaatag tagacatgtt tcaaccatcc caacagcaac aaataaaaac
    48841 acagttatct acagtttgtg aaggtgtggt atcacagcag ttacttccta cagcagatgg
    48901 gaaaggcaga atagcagcaa ttgaattgat gtttgctact ccagcaataa aaaacctgat
    48961 aagggaaggc aaaacatatc aaattccaaa catgatacaa actggagtta aagtaggtat
    49021 gaaaactatg gaccaagatt taatggaact ttataaaaat ggaaagatta caaaagatat
    49081 ggcacttagt aggtgtactg accaagagtt tatgacaaga atgataggtg gagtaaacta
    49141 caatggatat tataatcgtt aatctatatg tatttatggt gggtatagtg tttggaagtt
    49201 tctttaacgt ctgtattttt aggattccag aaggtatatc tatttctaga cctccttctc
    49261 attgtatgag ttgtaatact agactaaagc caatagactt aataccagtg ttaagctatt
    49321 taatgagtgg cagaaagtgt agatattgtt atgagaaaat ttctagtaga tatgctatag
    49381 tggaattaat tacaggactt ttatttttaa cagtatttat gttatatgga atatctacat
    49441 caactattta ttatttggtt cttgtgtctt tactgattat aattacattt atagatattg
    49501 accactttat aataccagat aaaatattgg tttttggagc tatattttct atgattttta
    49561 accttttatt taaagaaatt ccagttaagg attcaattct aggtgttttt atttgtggag
    49621 gaagtgtctg gattatagtt cttttgattg aatttgtaat aaaaaaagag tgtatgggtg
    49681 gaggagatat caaactattt gcaatgctag gtttttacat gggtgttaaa aatggtcttt
    49741 tgacagcctt actaagcgtt tatgtgggag cagtctatgg aatctgtgta ataattataa
    49801 gtagaataaa aggaaaggaa tataattctg ttataccata tggacctttt atatctattg
    49861 gagcattaat aacaatctta tgtggaaagc aattacttga actttatttt tctatggtta
    49921 tatagatgat agtcaagtta aataaagttt taatttaaaa aaatgaatta aagtatttaa
    49981 taaggacaat attattatta accctattac taaagtgcta caaactatgg agtatataat
    50041 aataatttcc ttattttttg cacatttgat aaataaatct ctatgtgttt tttgtgatag
    50101 agaaaagaaa aatttataca atacgttttt tatttacata ataactgtag ttttatctgt
    50161 attaatatat ctaaaatttg ggattagtgt agaatgtgta caatatatgg ttttaattcc
    50221 atttttaata attatatcca ttgtagatta ctacacaata tatgtatatg atataacaat
    50281 agttagtggt ataatagttc aaggtataat ttttttggtc tttttcaata tagttcaatt
    50341 tgattatata aatcatatat ttggactatt gataggcttt attatacctt atattattgc
    50401 taaactaaca aaaggattag gctatggaga tgtaggtctc tatagcttat gttgctttgc
    50461 attgggaaat aattatgggt tttatgtgat agttttatca tttattttag cgtttttgtt
    50521 ttttattatt ttatacataa ctaaaatatt taaagatgta gatataagaa agcaaattcc
    50581 ttttacacct tttatttcct tgtcaacaat tttaataata tttacagagt ataatatatt
    50641 taatttttat tataatttgt tatacaaatt tatataacag gaggtaagaa atgtacgtag
    50701 tagtagggtt aggtaatcca ggaaagaagt atgagaagac tagacataac gttggatttg
    50761 atgttataga tattcttgct aaagaataca atattagtgt tactaaaata aagcataaag
    50821 ctttgattgg tgaagggaga gtaggtactg aaaaagtatt attagttaaa ccacaaactt
    50881 atatgaattt aagtggagaa acattaatag atatatataa atattataag gttgatttaa
    50941 gtaatatagt agtagtatat gatgatatag atttagaagt tggaaagata cgtataagaa
    51001 aaaaaggaag tggaggaact cataatggca tgaaatctat aactaagtgc ctaggttcaa
    51061 atgactttcc aagagtgaga gtaggtgtat ctaaaccaga agctggtcaa gacttagcag
    51121 actttgtact ttctagattt agaaaagaag aatctgataa cataaatgaa gcattagaaa
    51181 aagcggcaga tgctattgac tcaataataa gagaaaatat agatatgtct atgaacaaat
    51241 ataatggata gtgagaggga gatttaaatg aatgatgttt ttttatatcc tttgcaaaat
    51301 tcaaaagagt ataaagatat aataaactgt ataaaaaata caaaaggctc attattggtt
    51361 aatggacttt taccagtaca aaaacctcat atatcgtatt ctatatttaa tgatttatca
    51421 cgacaaatga tttttataac aagtagtgat ttagaagcaa agaaagtata cgaagattta
    51481 agtttttata tggaagataa agttgaatat ttaggttttc aagatattta tttttatcac
    51541 ttggatgcaa aggacagaaa cgaagaagcg aagaaattaa aagtattgtt aaaactagca
    51601 aataaagaaa aaatcatttt agtaacttct attgaggcag ttttaagaaa atacatacca
    51661 aaacaagttt tgctcgatag tgtatctcat tataaggtgg gagatagctt agacttagaa
    51721 aagttaactg agaaattagt ttcattggga tatgaaagag tttcaaaaat agaggggttt
    51781 ggtcaattta gtataagagg tggaattatt gatgtatttt cacttgaata tacaaatcca
    51841 attcgtatgg aattatttga tgatgagata gattccatac gaacttttga cgtatattca
    51901 caaaaatcaa ttgataaatt gcaacaattc tctataactc catctaggga atttatttat
    51961 cccgaaaaaa ctacagatgc attagttaaa ttaaaaaaag aaacaactaa aaatactgat
    52021 gaaggtacat ttcaaaatat agattatata tcaagtaaga cttattttga aggtgtagag
    52081 aactatatag attatattta tccagaagaa aacaaaagta tatttactta tttagcagat
    52141 gatgcaatag tatttattaa tgatataacg agacttaaag aaagatgcga aaattatata
    52201 aatgagttta gagaaaatta taaattaaat cttgaaagag gtctggctat taaaaaccaa
    52261 ggtaaattgt tatatcacta tacagattta gagtacttag taaaagacaa gtctgtggta
    52321 ttaaactcgc tattgccaaa gtctataaat aacttttcta taaaatctat aataaatttt
    52381 gaaagtagag aagttcctac atttaatgga aaggtagatt tgcttgtaga agaattaaat
    52441 cggctgaaat ataatggata caaaataatt ttagcaacaa ataccttaga aagagcaaat
    52501 aaattaggaa aagatttatt ggacaaaggc ttagaaacga ccatttctaa agatagagat
    52561 attgaaatta aatcttcaca agttataatt gtacctgcac acataaacag tggttttcag
    52621 tataaatcta taaagtttgt tgttataaca gataacgaaa tgataggtgt ttataaaaga
    52681 gcgtctaaga ctagcaataa aaaagttaaa aagggaaaga aaatagaatc ctttttagat
    52741 ttaagtgttg gagattatgt agtccatgaa aatagtggtg taggtagata cacaggaatt
    52801 gaacaaatta cagtcaatgc tattaaaaaa gattatatga aaatagtata tcaaggtgga
    52861 gataatctat atgtgccaat agaccagatg gacaaagtac aaaaatatat tggtgcagaa
    52921 gtagaaaaag ttaagttaaa taagcttgga acaaatgagt ggacgaaagc aaaagcaaaa
    52981 gttaaaaaag aaattgaaga tatgacaaaa gacttgattg agctttatgc aaaacgtgaa
    53041 aaaatacaag ggtataagtt ttcaaaagat acaccttggc aggcagagtt tgaatcgttg
    53101 tttccatatc aagaaacaga agaccaatta aaggctatag aagaaactaa aaaagatatg
    53161 gaatcaagta aggtaatgga taggctagta tgtggagatg ttggttatgg aaaaacagag
    53221 gtagctatta gaagtatttt taaagcttgt atggaccaaa aacaagttgc tgtattggtt
    53281 ccaacaacaa tattggccca acaacattat aatacattca aagagaggtt tgaaaattat
    53341 cctttgagag tagaagtttt aagtaggttt aaaacaccaa aacaacaaaa acagattatt
    53401 gaagatgcta aaaaaggatt agtagatatt ttaattggaa cacatagaat aatatccaaa
    53461 gatataaatt taccaaactt aggtcttgta gtcattgatg aagaacaaag atttggagta
    53521 aaacataaag aagctttaaa gaaaattaaa tctacagtag atgtacttac actttcagca
    53581 actcctatac caagaacatt acatatgtct ttaagtggaa taagagacat gagtgttata
    53641 gaagaacctc cacaagaaag acatcctgta ataacatatg ttacagagag taaggaaagc
    53701 gtaattcaag atgagataga gagagaatta agtagaggtg gacaggtatt ttttgtatac
    53761 aatagagttg aacacattga agaaatggca agtatgattc aaaaactcgt accagatgct
    53821 agagtggctg ttgcacatgg aagaatgact tctaaatcat tagaaaatat aatcctagga
    53881 tttttaaata aagattatga tgtactagtc tgtactacaa taattgaaac tggtatggac
    53941 atatcaaatg caaatacaat gattatatat gatgcagata aaatgggatt ggctcaactt
    54001 taccaattga gaggtagggt tggaagaagt tcaagacaag gatatgcata tcttttgtat
    54061 gaaaaagata aaactttaag tgagatagcg gagaagagat taaaagctat cagagaattt
    54121 acagagtttg gttctggttt taaaatagca atgagagacc ttgaaataag aggagcagga
    54181 aatattttag gttctcaaca acatggtcat atggcagtta taggttatga tttgtacgtt
    54241 aaaatgctta atgatgcaat taaaaaagtc aaaggtgagc ctattgtgga ggaaattgat
    54301 gttgaaatag atttaagtgt aaatgcatac attccagata attatatcaa agatgagttg
    54361 attaaaattg aaatgtataa gaaaatagct tctattgaaa acaaagaaga catgttggat
    54421 atacaagaag aacttgagga tagattctct gatataccta aaccagtcca gacacttctt
    54481 acaatagctt atataaaatc gttatgtaag atattaaaaa tagaaaagat aagacaatta
    54541 aaagatgaaa tattattagt tccaataaca aagtatagaa ctaaacagaa aataggttat
    54601 aatatagtta cagaattaga agaattatta gaaaagatgt gcaaggtaaa ataacttgta
    54661 aatttaagaa gggatgtttt aaatttgaaa aaagtaataa cattggtaat agctatgatt
    54721 ttggtagtat ctgtgactgc ttgcagttct agtaaaggag aaactgtggc aacagttgaa
    54781 ggtacaaaga tatcatcaga tgaatttaaa aaaactatag ctctttataa agattctatg
    54841 gaacaaactt atggaaaaga tatatgggat aaggaagttg aaaagggtgt aaaatacaag
    54901 gacaagttta aagatttaat tcttgaccaa cttataacta cagaagtaat atattctcaa
    54961 gctaaaaaag ataacttact tcctaaaaaa gaggatgttg aaaaaagttt taaagaatta
    55021 aaagacgcta tgggtaaaga tgaaaaatat aaagaacaac ttaagaaatt aggtatagat
    55081 gatgagtttt taagagacca acaagagaaa gatttagcaa tgcaaaacta tcaaagtaac
    55141 tttgctaaaa agactaaaat atcagatgaa gaaatgaaaa aatactatga tacacataaa
    55201 gatgaattta aaaaagatga ggtagaagct tctcatattt tattgaaaac agtagatgat
    55261 aataacaagc cactttcaga taaggaaaaa gctgaagcta agaaaaaggc tgaagaggct
    55321 cttaaagaag ttaaatcagg tgaggatttt gcaaaagtag caaaaaaata ttcacaagat
    55381 acttcagcaa gtgatggtgg taaattagga ttcttttcaa ggggtcaaat ggttgctgaa
    55441 tttgaagatg ctgctttttc tatgaaaaag ggtgaagtat ctgatttagt tgaaactcaa
    55501 tatggatacc acataattaa agtgacagac agaataaatg agcaaacatc atttgaagat
    55561 gctaaagaaa ctataaaaga ccaactgttg aaaaataaat atcaagaaca aatagaaaaa
    55621 ttaactaaag aagctaaggt agaaaaagat gaaaaagtaa taaataaaat cacaatctaa
    55681 gacttaaaaa aagagtaata tgcttaaaaa agctatatct ggataagata tagctttttt
    55741 tgtatatttt tcttaacaat ggtaaaaata aacttaaagt aatttatgga gggattaaaa
    55801 aatatgaaag caacaggtat agttagaaga atagatgatc ttggaagggt agttatccca
    55861 aaagagataa gaaagacatt aagaattaga gaaggagacc ctttagagat attcacagca
    55921 aaagatggag aagtcatact taaaaaatac tctccaatag gtgaattaaa tgaattttcg
    55981 caagaatatg ctgaaacatt agctgaagta ctaggatatg gagtgttgat aacagactta
    56041 gattctataa tagctgtgtc taagcttcct aaaaaagatt ataaagaaaa aagtataagt
    56101 gatgagttag aagcaataat aagtgaaaga acagtaaaat attcaaaaga taaaaacatt
    56161 ttaccattat tcaaggaaga ccaaaaagaa tatactagtc aagttatcat gcctataatt
    56221 agtgcatctg gtgactgtat aggttcaatt gtagttgttt caaaggataa agatacaatt
    56281 gcagaaagtg aagaaaaatc tttaaaaatt gctgctaact ttttaggaaa acaagttcaa
    56341 taattttatc ataaagacct tttacatgaa ggtcttttga agtttttgtg taaaatattg
    56401 aatatagcat taaaaatatg gtatattaaa gtttgttata taaacagtat ggaggttatg
    56461 ttatgaacaa taacaaaaat aaagactcct ttctaaaagg agcccttata cttgggctag
    56521 caggtgtaat cgtaaaaata atgggtggat tttttaggat tccccttgga aacatgattg
    56581 gttcagaagg aatgggatac tatcaggctg cttatcctgt gtatactcta tttttaacat
    56641 tagctactgc tggatttcca acagctgtgg caaaattggt ttcggaaaaa gttgcaattg
    56701 gaaattttaa aggtgcaaat aagatattta aggtatcaca tacagtttta ttcattaccg
    56761 gtataatatc attttgtata ttattttttg gagccgacta catagtaaca aacgtaatga
    56821 aaaatccagg tgcgttatac tctatgaaag ccattgctcc agcattacta tttgttcctg
    56881 caatgtctgc atatagagga tattttcaag ggagacaaga tatgactaag attgctgtgt
    56941 ctcaagttgc agagcaattt tttagagttg tattaggtct tactttggct tacttgctta
    57001 tgaagagtct aggtcagact tttggtgcag caggtgctat atcaggggct acaataggtt
    57061 caatagcatc tatgctttat ttggtgtttg cttatatgct agggagaaaa gagcgtagag
    57121 cagagataga tgcaagccaa agatttaaag atgaaagagt gtcttatatt tttaaaaagc
    57181 ttttaactgt tgccattcct ataacaatag gagcatcagt aatgccactt gtaaatatga
    57241 tagataatgt tattgtaata agaagactta tggaagcagg ctttacatac aaagtggcaa
    57301 attccatgtt tggtcaatta acaggtatgg caatggccac aataaattta ccagcagtca
    57361 taacaactgc aatgagtatg agcttagttc cagcaatttc taaagcatat gcattaggaa
    57421 ataagtcaaa agctagaaaa gatactaaga gtgcagttaa ggtaacttta cttatagtat
    57481 tgccttgtgc gtttggtatg gcttctttag caataccaat aatgggtctt ttatttccac
    57541 atgaaccatc ttctgtagga actattttgt ttactcttac tccatgtgta ttatttttag
    57601 gattaataca gacattgact ggtataattc agggaatggg aaaaccaata gtacctgtaa
    57661 ttgctttatg tgttggtatg ctatgtaaaa ttgtaataag ttatacttta acaggtatac
    57721 cagatattaa tgtactgggt tcagcatttg gtacagttac agcatacttt gtggcagcca
    57781 tgattaactt actatatgtt aaaaaacaca tgaatgtaaa cttttctaaa aaagaattta
    57841 taataaagcc atttataaca gttatgacaa tgtttataat ggttaagttg agttatggtg
    57901 ctttagttgg attcttagga aacagcatat ctacaataat tgcaatatgt gttggtggaa
    57961 ttgtatatgt agtagtaata cttggaatag gtggaattaa aaaagaagag attctaacta
    58021 tgccaaaagg tgataaatta tataaattgt taaaaaaagt taaacttata agataatata
    58081 ggaatatata tgcaaataaa ggaaatacta tacaaatagt aaatctcctt atttgtataa
    58141 taatataaaa ataaagtgaa atttgctaat aggagtgatg ttatgggtaa gataagtatt
    58201 gtaggtcttg gaccaggaga ctattctctt ataagccaag gtgtgttaga atctttaata
    58261 aatagcccta aagttttttt tagaacagag aaacatccaa taattgataa acttaaagaa
    58321 actatacaat acacttcttt agactacttt tatgaaaaag aggaaaattt tgataatgtt
    58381 tatttgaaaa tatctgaatt tatcatagaa gaatcagcaa agggtgacct agtgtatgct
    58441 gttcctggtc atccaagagt tgcagagaag actgtaagta taatagagag attagccaat
    58501 gaaaagcaca tagcagtaga aatattgcct tctatgagct ttgtagacgc tatgtttaac
    58561 tatttggcta tagacccatc tgagggattt aaactggtag atgcttttga aatagaaaat
    58621 tcatatatag acttggatac aagtatgata ataactcagg tatatgataa atttatagca
    58681 tctaatgtaa agttaaaact tatggaacat tatgactata atcaagaagt atgtatagta
    58741 aatggagctg gtgtcaaaga tttagaaagt aaaaagttta taaagctttg tgaattagac
    58801 agaaatgaaa acttttttga ctatttaact agcttatata tacccaaaag ttctaaaaag
    58861 atgtataata cagtacatga cttagaaata atagttaata cacttagaag tccatctgga
    58921 tgtgaatggg ataaaaaaca aacacatcaa tcattaaaaa attctgttat agaggaagca
    58981 tatgaacttt gtaatgcaat tgataataat gatatagatg aaatggtaga agagttaggt
    59041 gatgttcttt tacaggttat attccattgt cagataggaa atgaggaggg ctactttgat
    59101 ttaaaagaag tagtaaatgg catatgtaaa aaattaattc atagacatcc tcatatattt
    59161 agtggtgcaa aattagatat gtctaagttt gagaaaactt gggaagaatt aaagaaagaa
    59221 gagaaaggcg aaagtactat aacagatggc cttagaagga taccaaaaca tttaccagct
    59281 cttatgaagg ctagtaaagt tcaacataaa gcagcacttg tcggctttga ttgggacaat
    59341 gtagaagatg tatttaaaaa aattgaagaa gaatatcagg aattacttga cgaacacagg
    59401 gtcgggaata taaaatacat aaaggaagag ctaggcgatt tattattttc gatagtaaat
    59461 ctagctagat ttttagacat agactcagga gaagccttga actgtactac agaaaaattc
    59521 ataaatagat ttgactttat agaaaagagt gcaatgaact tgaataaaaa attagaagac
    59581 atgacacttg aagagatgga tgagttttgg aatcaggcta aaaaacaata aaattgtgga
    59641 aggaaatttg tcaaaaatct agaactttta tcacaaagat agaagtctat attataaaca
    59701 tttttaggag gtaaatatcg tgaataaagc tgaattagta tcaaagatgg cagaaaaaag
    59761 tggattaaca aagaaggaag cagaagctgc gttaaacgca tttatgagtt ctgttcaaga
    59821 tgcactagta aataatgaaa aagttcaatt agttggattt ggaacatttg agacaagaga
    59881 aagagctgct agacaaggaa gaaatccaag agatccagag caagttatag atataccagc
    59941 ttctaaagca ccagttttca aagctggaaa aggattaaag gatataataa atggataatt
    60001 agaaatcttg tgaaaaagtg tggataaatt tgccacactt tttctataga accaaggaag
    60061 gtgataatta tgagattaga taaattttta aaagtatcta ggattataaa aagaagaact
    60121 gttgctaaag atgcatgtga taaagggatt gtcactataa atggaaaagt tgctaaatct
    60181 tcttcagagg tgaatattgg agatacatta gaaatacaat ttggagagaa gaagatgaag
    60241 tttaagataa atgaagtaaa agagcatgta ttaaaaaatg atgcaaaaga aatgtacgaa
    60301 attttagaat aaaattaata gtcctctagt atattaatag tagtattatt gaaatattta
    60361 ctggggggat attatggaac agaacataac tttgaaagat agatcaaagc tggttatttc
    60421 gggagtcgag cacatatact cttttaatga caaaagagta gagctcaaaa cttctgtagg
    60481 cgagatggta attgaaggcg aaaacctaga tatgagcaag ctaagtatag acgaaaatat
    60541 aattagcata gatggaacta taaattctat ggtatatgca aagcctaaaa agccacaaga
    60601 gagtttcttg aaaaaggtat ttagatagat gatacctttt actcaagatg taagcatttt
    60661 ttatgctact atttatggtg gaatactcat aggggtgttg tttgattttt atagagggct
    60721 aagagggaat tttaaattca tcaattattt tgctataata tttgatgtat tgttttggtt
    60781 tctagctacg gttataatat tcgtaacaat aaaccttaca gaattttttg atttaagata
    60841 ttatcatttt gtggctttgt ttattggttt tattttatac tataacacca taagtaaaat
    60901 tgttttatca attatcaata aaataattcg ttttgtgaga aattctttta aaaaagttac
    60961 acattacata gtcagttttt taaataattt gtattacgtt ataatctatt cactacacct
    61021 actatttgat attatatttt atataccaaa catatttata gcaacaagaa aaagtataaa
    61081 aagaaaatca aataaaaaat taaagaacaa gaaaaaatca aaacctaaga agaaaaaaaa
    61141 taaaactaaa aaaagggtgt aggaaaaatg aacttaagaa aaaaattttc aggacagatt
    61201 attgttatca gtttattttt aggaatatca atttttagta tgatgacagg atttgtattt
    61261 gagtatacaa aagctaagga atataaaaaa gaaatagcaa gtctaaataa acaattaaaa
    61321 aagactgaaa tacaaataaa cagcttaaag aaagatgaaa aatcatatga aggagactta
    61381 gaggatatag caagaaaaag attgaatatg gtaaaaccaa atgaaacagt ttatgtagac
    61441 ataaatcgtt gattaaaact tgctaataag tttacagatg tattgatttt aaaatttaag
    61501 atttaataaa aaagtcattc agttaaggat ggctttttgt ttttattgag gagggaattt
    61561 cataatgaaa gtaggagcta tagatattgg gacaaactca atgagattat tgatagcaga
    61621 ttatagagat gggaatttta taaacagaaa aaaatttgtt aatacaacta gaattggtta
    61681 tggtgtggat agtaaaggat atataaaaga agatgctatc aaaagaaata taaaagcatt
    61741 aaaagaattt tctgataagt gtaatgatga aaagtgtgaa aaaatttatt gtatggggac
    61801 atctgctctt agagacagta aaaatggaga ttactttgtt aaattagcta aagaagaaac
    61861 tggaatagat gttgatatag ttacgggaat agaagaatca aatttgggct ttaagggtgt
    61921 tttagaagga atacaaggtg atgatactat tattgttata gatattggag gaggttcaac
    61981 agaatttatt gtaggtgatt cagaaggaat tatatttaat aaaagtgaga atgtaggtgc
    62041 tcttaggatg acagagaggt ttttgaagca cgatattatt gaagatgaag agtttgaagc
    62101 tatgagttca tttatatatg gtgagattga taaaacgtta aatcatataa aagacaaagg
    62161 attaaaaact cttataggta taggaggtac gattacatca ttatcagcaa tgaatcaaga
    62221 attagagatt tactctatgg aaaaaataca taatagtaaa atacacaaaa aagaaataga
    62281 agaaattcta caaaaattaa taaagatgac attaagtgat aaaaagaact taaaagggct
    62341 acaagaaaaa cgtgcggata ttataactgc tggtgtaaaa atattgaata taataatgga
    62401 aaaattagaa atagaagaaa taaccgtaag tgaatacgat aatttggaag gcttaatatg
    62461 tcaaaagtca aaaaaaatgt attaatattt tttgattttt aaaaccgatt atgacaaaaa
    62521 aaattactcc tctttgttac aattcttaat ataaacaaaa gaggaggttt ttttatgcaa
    62581 agaagtgtag cagttgtgaa gaataagact gtaaaaccaa atgtcataaa attaataagc
    62641 ggatatattg attttactac ggcggcattc gctgtgatgg gatttttact tagtaggtct
    62701 attatagtag actctgtagc tcctttagga attgcctttt ttatatgtgc atcaaagatt
    62761 gataagtaca agatacctgt attcatatca acattattag gaatattgtt atcatctaat
    62821 gattttggaa atgtagcaaa atatgctgtg tgccttacaa tatttatggc tatgaataaa
    62881 aaattaaaat tatcagattc aattgctaag gttgcattta taggtgcaat aatactactt
    62941 ccaatctcca tgggacaagc tttcttttca aataggtatg tatatggtct tcttatagca
    63001 ggagtagaag caataataat gtttatatct atatatgtgt tttcttatgg gataggttta
    63061 atggccaatg tcaacaatag gatgtctgta cggacagaag aagccatatc aataagttta
    63121 ctgctaacct ttagcataat gggaatagga gatatatctc tgtttggaat atcaataagg
    63181 accattttat cgaccgtatt gatattgctg acctcaatag taggtggagc tactatggga
    63241 gcatctagtg gtgtaattat tggtgtagca tccattttaa ataatatgac atcagctgtt
    63301 tatatgggga tatattcttt ttcaggatta ataagtggag catttaataa aataaataaa
    63361 tatttctgta tactaggata tattctaagt tggacaataa tatacctata tacttctggt
    63421 attacttcta atatgatgca attaagagat atactattag gttgtttgat agtactagta
    63481 ttgccagagc gattatttaa taagattgaa aaattaataa aaagtaatgt ggcttcaaat
    63541 gaaatcgtat acgattatat tatgagaagc aaaaacctaa ctaatagcag attgaacagt
    63601 atttataaaa cttatgatga ccttgcagat acatttgata agataagaga aaaagacaaa
    63661 gtgctagacc aaagagacat agctaatgta atagatatga ttcataatga tgaatgtaaa
    63721 agttgtagta tgagaagaat gtgctgggag tcaagattta atcatacata cactatgata
    63781 tataacattc ttgaaaaaat tgaggagaaa ggggagttaa gtctaaacga tatacctaaa
    63841 aattttagaa aggaatgtat gaaaccagag tcaatagtga aaatatcaaa tcactattac
    63901 aaaatgtttg tattagatta tgattggagt gtgaagtttt cagaaagtag aaagttaata
    63961 gcaaatcaga taagaagtat ttcaaaatcc attaaatcct tatctcaaga tttagaaggc
    64021 gacataatgc tagatataga aaaggaaaaa aatatatatg aacagttaga aagatatgat
    64081 ataacagttg ataaagtaag ttacttaaca aaaagtaaca gtgagtttga aatatcaata
    64141 gaaaagaaaa cttgccatga tggatgtatg tgtgaagata aaattgtaaa tataatttca
    64201 gacctagtag gggaaaatat gtctgttaga aaaataggat gtcactgttt aggaggtaaa
    64261 tgcaaggcaa cattcgttaa atctcaaaaa tataaggctg ttacagaagt ttcagcaatg
    64321 tcaagggatg ggcatatcct ttgtggagac aattacacat acatggagat aaatgatggt
    64381 aaatacatga tggctataag tgatggtatg ggaaaaggta aaaaagcata tgaagaatct
    64441 tctgccacta ttgatatact ggaaaaaatg atagatgcaa aaattaaaga tgagatagtc
    64501 attgatacta taaataatat gttgttgtta aagtcttcag aagagatgtt ttcaactctg
    64561 gatttgggaa ttttagattt aaaacgaggt tgtttagaaa ctataaagat gggagcgtgt
    64621 tctacttata taaaaagaga ggatggagaa gttgacttaa tatcgtcatc atcattacct
    64681 gtgggtatat tatcagatgt aaaaattgat agaaaaaatg taaaagttaa ggaaggagac
    64741 tatgttataa tggtttctga tggaattgta gatgcaggaa gaaataacaa tttaggagac
    64801 aattggttaa tatacttctt aaagaatata gagactacaa atccaaagga aatttcaaat
    64861 ttaatattgg atagagcatt agagttacaa gcgttacaaa ttgaagatga catgactgtt
    64921 ttagttacga aaatatgtac aaaataaggt ttacaagatt atgtgtataa gatgtggata
    64981 acttgtgtat aatatgttaa tagacttttg aaaaaagtat agttattaac aaaaataaca
    65041 tataaatcaa caaaatttgt tgataagtat ggcagaaatg ttaataaaat gtaaaaaaag
    65101 aaaaaacaaa agctattatc aaaagagatt catgttgaaa taactaaatt tctaagtaaa
    65161 gaaaacctgt gaatatgttg attattatgt ggataactat taattcgata tttatttata
    65221 tagtatgaat aattgaataa tatatctaaa aaaagccatc ctattattaa gtaaacttat
    65281 tgaaaggatg gctttttatg aatagaaaat tgtgcaaagc aataatacct ttatttattt
    65341 ctattttttg catgttaata ataagtataa aagctgacca aggatatcaa aagaataatg
    65401 atagagcaat tactgtcatg aataataatg atactgaaga tgatgctgaa aaaaaacaag
    65461 aaatagacta tgaaaaaaca tgttggaatt tgcaatcttt gtttaaaagt gatgaccaat
    65521 ggaaaaaaga acttaagagt tttgataaag atactaaaga gttgaaaaat tacataggta
    65581 aagtaacaaa atcgccaaca catttgtctt ttgccttaaa cattaaagaa aaacttgata
    65641 taagactaaa taaactttct gcctatgtaa aattaaaaca agatactaat aaaaattcgt
    65701 ataagtactt agatatgaat gatagtatga gtaaatccta tgggaattat atggggattt
    65761 gttcagattt agagttagaa atactaaaat tatcaaacaa agattacaag aaaattattt
    65821 caaataaaaa tataaatagg aaatatggtg catatttaag agatataaga aggaataaag
    65881 tacattattt ggatgataaa tcagaagata ttctaagcaa agtatcttca ataagtagtc
    65941 ttccaagcca agtatatgaa ttatttagaa atatggatag aaaaactaat ttgacaccag
    66001 ctcaatatgc gagtgaatta gagtcatcag atagagaaaa tagaaaaaaa gcttatcaag
    66061 atgaacttat aacttacaat gataatataa atacaattgc aggtttaatt actggtcaag
    66121 ttaaaaagaa tattttttat tctcaggaaa gaggttatga gtcttcttta gaaatgtacc
    66181 tggaatctga taatgtagat gaaaaagtat acaataattt gataaggact gtaaatagta
    66241 atatggacag tctacacaaa tatatagatt taagaaaaaa agtattaaaa ttagataagg
    66301 ttcattcata tgatatgttt gtgcctatgg ttaaggctgt tgataataat attaattatg
    66361 aaaaggcaca aagtatagta tattcagcgt tgtcaccttt aggaaaagaa tacaatgatg
    66421 tagtatataa agctttcaat gaaaaatgga tagatgttta ttcacatgaa aataaagtaa
    66481 gtggtggata ctgcttatct gtttatgaaa atcatccata tatactttta aattataata
    66541 attcattagg ttcagtttcg actttatcac atgagcttgg acatgctgta tatgaatatt
    66601 taagttctaa gaatcagaat tattttaatt caaatccgtc tatatttact cacgaagttg
    66661 catctacaac gaatgaagca ttactttacg agatgcttat aaaagaagct aaaggcaatg
    66721 atgaaaaagc ttattatata acacagtatt tagacttgat aaaagatact ctttatacac
    66781 aaactatgta tgctgaattt gaaaaaacta tacacgagtt agtagaaaaa ggaaaaagtg
    66841 taaacgcatt agtattaaat gatgtttggg gacaacttct taaaaaatat tatggacaaa
    66901 gctatgaatt agaccaatta tctaaggtcg gatgggctag aatcccacat ttttataata
    66961 gtttctatgt ctataaatat gctacaggtt gtagtgcagg agtaagtttt gcccaagaca
    67021 tcctaaagaa tggttcagat aactatatga gctttcttaa aaagggaagt tcagattatc
    67081 caataaactt gttaaaatca agtggcataa atttaactaa tacaaaaccc atagaaaata
    67141 caatcaaaaa atttgatagt ttagtagcag aacttgaaaa gctattataa acattattgt
    67201 aattcacatg gtaaatatat cgaattaatg ataaaatata ctaaaagttt aaagtcctat
    67261 caagatagga ctttttattt attaattagt gggagatgat tctatgcaag ttaaagttaa
    67321 aaaggctgta ataccagcag caggtttagg aactagattt ttgccagcta caaaggcaca
    67381 accaaaagag atgttaccaa tagttgataa gccaactcta caatacataa tagaagaagc
    67441 tgtggcctca ggaatagagg aaatactaat aataacaggt agaaataaaa aatctattga
    67501 agaccacttt gataaatctg tagaattgga acttgattta gagaaaaaag gtaagaaaga
    67561 attgttggag gtagtacaaa acatatctaa catgattaac atacattata taagacaaaa
    67621 agagccaaaa gggttgggag atgcaattta ttgtgcgagg cattttatag gtgatgagcc
    67681 gtttgcagta atgttagggg atgatattgt agataatgat gtaccttgtc taaaacaact
    67741 aacagatgca tatgaggaat atagaactac aatattgggt gttcaaaagg taaatcaaga
    67801 agatactaat aaatatggga taatagaagc aaaaaatata gaaggtagag tttacaaggt
    67861 aaaagacatg gtagaaaagc cagagtcagg aaaagctcct tcaaatattg ctattttagg
    67921 tagatatata ataactcctg aaatctttga tattcttaag gatttaccac caggtaaagg
    67981 tggagaggta caacttacag atgctttaaa aatattatct aagaaagaag ctatgtatgc
    68041 atacaatttt gaaggcaaga gatatgatgt tggggacaag ctaggatttt tagaagctac
    68101 tgttgacttt gctctaaaga aagaagacct taaggaagat tttataaaat atttgaaaca
    68161 tgtatgtagt gaatttgata aaaataacaa tgtgttatac aataatacag atgaaaaaat
    68221 agatttaatt gaagtcaatg aagctagaga acttgaaatt caaaaataat taatgaaagg
    68281 tgatgtatgg tggcaaaatc aagtcagaaa agaattaata gaataatagc tgttgggatt
    68341 gtgggaatcc tcgtaataac gagtataaca gctgttttga gtatgattgc aactatgtaa
    68401 taagataaat gacagcagtt gatatacata aatataaaaa ccatatataa cttgatgtag
    68461 tcaaaaactt acaatatttt agttatatat ggtttttaga atttataaat gtaaaaaaaa
    68521 atttttataa tagttgcata ttttaaagga aaatattatt ataagatata aatatagtga
    68581 taaatttata aaaacgtgct aagattggac ttgaaataca gtaatatagt aggtaataat
    68641 gttttaaatt ttatttattg accataatat ataaataatg atattattag acttaactgt
    68701 ttaaataaaa cttttctttt tcatgccttc aatttccaaa ataagtatgt atataaatga
    68761 atatatagat gaggaggatt tttttgattt tagatgataa agtattagaa ggattaggta
    68821 gtaagacctt agttgagcta cgagagatag ctaaagaatt aaaaataaaa tcaattacta
    68881 catataagaa aaatgagtta attgagatta taaattccaa aagtaaaaat gaggaaaaag
    68941 taaacgaaga gactaaagaa aaaaatgaag atattaaaga agacataaag aatagaaaag
    69001 aaaatataga gcgtgaaagt atagaaaaag aagttgaata taagagccct accaaatcat
    69061 acaacaagaa agaaatagat acccaaaata tttcccaaaa ccaagaacat agaaatcaaa
    69121 gaaatgacta taataagaca acgagtgtaa atgatagtaa taagtcatca aatatatcta
    69181 atagaatggt cagaaataat aataaaaact attatatgcc taaacaagtt gatgaatcta
    69241 agatagtaga tgaatttaac acatcaaaag aggatgaagt agtaggtgta cttgaaattc
    69301 tgccagatgg atttggtttt ttaagaggtt caaattattt gtctactgaa ggagatgttt
    69361 acgtttcacc atctcaaata agaagattta atatgaaaac aggagacaag attaagggaa
    69421 taactagaca tccaaaaagt ggagagaaat ttagagcact tttatatgtt caaaaaataa
    69481 atgatgaaaa ccctgacaca gctatacaaa gaaatgcgtt tgagacatta acaccaatat
    69541 ttccagaaga acgacttaca ctggaaacta atagaaatga aatagctaca agaattatag
    69601 atttaatttc acctatagga aaaggacaaa gaggattgat agtagcacct ccaaaagctg
    69661 ggaaaacagt tcttttaaaa agcgttgcaa atagcatagc taaaaatcat ccaaatgttg
    69721 aacttatagt actccttata gatgaaagac cagaggaagt tactgatatg aaagaatcta
    69781 ttgaaggaga cgttatatat tccacttttg accaagtatc aagtcatcat gtcaaggttg
    69841 cagagatggt gttaaataga gctcaaagat tagttgaaca tggtaaagat gtagtaattt
    69901 tactagatag tataaccaga ttggcaaggg catataacct tacaatatct cctacaggta
    69961 gaactttatc tggaggtatt gaccctggtg cacttcatgg acctaaaaag ttctttggtg
    70021 cagcaaggaa tattagacag ggtggttctt tgacaatatt aggaacagca ttggttgaaa
    70081 ctggttctag gatggatgat gtaatatttg aggaatttaa aggtacagga aatatggagc
    70141 tacatttaga tagaaaatta gcagaaaaaa gaatattccc tgcaatagat atttacaagt
    70201 ctggtacaag aagagatgat ttattgcttg atgatgaaga aaagactgct ctttggagat
    70261 taagaagaga aatgagtaat aattcagtaa tggaaattac agataaagtc attgaactta
    70321 taaagagaac taaggacaat aaagagtttg taaaatcaat aaagaatttg taaaatcaat
    70381 aatacaaatg caaatatgga ttatataaac taatagaaaa atcacttgaa tggcataata
    70441 agttgtgcta taataatgta gttgataatg taaaaagaaa gttaatataa ttaatttttt
    70501 taaataaaga gaagaggtga ctataatgca aaaggaaata caaccaaaat acaatccagt
    70561 tgaagtgcgt tgtgcttgtg ggaacacatt tgttgctggt tcaactaagg acgaaataaa
    70621 agttgaaata tgttcagagt gccatccatt ctatacagga aaacaaaaga acatagaaaa
    70681 aggtggaaga atcgacaaat tcaagaaaag attcaaaatg gactaatttt tataaaagtg
    70741 gagttggagg aagccaactc ctattttatt acaaaaaaac attcatattc tactttaaat
    70801 tgtctttttg tattataata ataaatatgt aaaattgagt attctaaata caataaaatg
    70861 aggtgagtgg atggcaaaac aagctgtagg tgggcaagct gttattgaag gtgtaatgat
    70921 gcagtcaaaa gacaaaagag cggtagctgt tagaaaaagt gacggagaaa tagcactaaa
    70981 agaagataga ataaaaagtt gggtaagaga taagaatata gataagatac cttttgttag
    71041 aggttctttt gttatgatag atacaatgat tcaaggcata aaaagtttaa acttttcttc
    71101 agagtttttt atggaagaag ctgaagagga taaatttgat ttatttataa agaaaatttt
    71161 taaagataag gcaaatgata taattataat attttcattg gtcatagcaa tgttactttc
    71221 tgctggatta ttcattttta tacctacatt ggtaggagga gctttttcta aagtaatgcc
    71281 taatgatttt atgcttaatt taatagaagg aatcataagg atagctatat tatttgcata
    71341 tatagtattg atttcacgaa gtaaggatat agaaagagtc tttcaatatc atggagcaga
    71401 acataaatct atatattgct atgaaaatga tttagaactt acagtagaga atgctagaaa
    71461 atttaaaaga ttacatccta gatgtggtac aaatttccta tttatagtta tggctgtatc
    71521 aataatattg tttgcatttt ttggttggcc aaatccaatt cttagaatat ttatgagaat
    71581 tatatgtgtg cctatagtag caggtttatc ttatgaggta attagagttc ttgggaaata
    71641 tgataatgga tttactaaaa ttattgcata tccaggaatg atgttgcaat attttacaac
    71701 aaaagagcct gatgatgagc aattagaggt tgctttggaa gcattgaaag cagtagtaga
    71761 ttagagaaaa ggatatttgt tatgacgata aaagatataa ttattaaata ttcagataaa
    71821 ctaaaagata taagtgatac tccaagatta gatacagagt tattgttaca aaaaacattg
    71881 ggtgttgata gattatatat tcacttaaat ctgaataaag agctaactga agaacaaaaa
    71941 acaaaattta tgggttttgc agaagaaaga ttaaatggaa gaccaatagc atatatagtt
    72001 gaaaataggg agtttatggg attagatttt tttgttaagg aaggcgtatt gattccaaga
    72061 ccagatacag aaactttagt agaagaaata attgaaatat gtagagaaaa aaaagatgta
    72121 agtatattag atataggaac tggttctgga gctattacaa taagtttagc aaagtacatt
    72181 gaaaattcta aaatcatgtc ttttgatata tcagagacag cattagaaat agctaaaaaa
    72241 aatgctatta taaatgaagt tggtgaaaaa ataaagtata ttaattctga tttatttaca
    72301 gcaataagtg atagtaacat aaaattcgat atcatagttt caaatccacc atatattaaa
    72361 aaacaagata tagaaactct gcacaaacaa gttaaagatt atgaaccata taatgctctt
    72421 gaaggtggag aagatggatt agacttttat agaagaataa cagaacaagg taagaaatat
    72481 ttaaataagt gtggaatatt agcatatgaa gttggtcata atcaagctga agatgtaata
    72541 aatattatga aaagtaatgg atataaaaaa atatacacaa agaaggatat tcaaggcatt
    72601 gatagagttg ttataggtta caatatatga tataatcaac aattgatatg ttacatttaa
    72661 tcgaggtgaa agtatgttaa aaaaattaga ggtattggaa gatacatata aagacttaag
    72721 tgaaaaaata ggtgatccgg atgtaataaa tgatcaaaaa gtttggcaga aatatataaa
    72781 agaacatgct gacttggaac ctattgttat gaaatacaga gagtataaaa gcgttttaga
    72841 tagtataaaa gaatcaaaag aaattttaca agaagagtca gatgaagaat taagagaatt
    72901 agctaaaatg gagttagctg agatggaaga aaaagtagca cctctggagg aagaaataaa
    72961 aatactatta ttgcctaaag accctaatga tgataagaac gttatagttg aaattagagg
    73021 tggagctgga ggagatgaag cagctctatt tgctggtgat ttatttagaa tgtactcaag
    73081 gtatgctgaa agaagaagat ggaaaataga attattgagt gcaagtgata ctggagttgg
    73141 tggatataaa gaagtatctt ttatgataaa aggtaaaggt gcttattcaa gattaaagta
    73201 tgaatctgga gttcatagag ttcaaagaat accatcaact gaatctggtg gaagaataca
    73261 cacatcaact tctactgttg cagtattacc agaagttgaa gatgtagagg ttgaaataaa
    73321 tccaaatgat ttaagaatag atgttttccg ttcttcaggt aatggaggac aaagtgtcaa
    73381 tactactgac tctgctgtta gggtaacaca tatacctaca ggtgaagtag tatcttgtca
    73441 agatggaaaa tcacaattaa aaaataaaga gcaagcttta aaaatattaa aagctagact
    73501 ttacgataaa gcattggcag aacaacacaa agatatagca gcagaaagaa aaagccaagt
    73561 cggaacagga gacaggtcag aaagaataag aacatataat ttccctcaag gtagaataag
    73621 tgaccataga ataaatttaa ctttatataa gttagatgca ttcttagatg gagatataga
    73681 tgaaatgata gatgctttaa ttactgttga ccaaacagaa aaaatgactg caatataagt
    73741 aatgttttaa agaagctgtt tcttcctaga aattattttc tattttgaaa cagctatttt
    73801 gcattttagg tcatgttatc atattctatt gtatataatt gttatataca agtataggag
    73861 ggttatatga tactaaaagt aacaataatt gggcttttgg caggtgttat agggacagga
    73921 ttaggaggag taatatcagc catttttaaa agagaggtcg ataaatatct aagttttttt
    73981 atgggattat ctggtggaat aatgttggct gtagtggtgt ttgatttaat gaaagaatcc
    74041 atggacaaaa tggggataat caatacagtt atatttacgt ttgttggagc tctcataact
    74101 atgtacataa aaacaaaatt agatgtgtct ggaaatatgg catctggata tcttatattc
    74161 ataagtatat tactacataa tttaccagaa ggactagcca ttggttcttc ttttatgtca
    74221 actgaaagtt taggtattac acttgctata gttataggtc tacataatat accagaaggg
    74281 ttagctatgg cattaggctt agtttgcaat aaaatgaaac taagtaaagt tattttgttt
    74341 actgtaatag ctggattacc tatgggtcta ggaagttttt taggtgttta ttttggagga
    74401 gtatttactt ctcttatagg agtatttctt gctacagcag gaggtactat gatgtatgtt
    74461 gtattggaag aaatttttcc acattcaaaa agtgtgtatt gtataattgg tttcttgtta
    74521 ggtacaatga ttgtaaatta tatataaaat taaaatgaaa gcaggcatac aaaagatgaa
    74581 aactattata agtaatattg atataaataa tattgattat gaggaaataa aaatacaagc
    74641 aaaacttctt agagaaggta aaacggttat ttttccaact gagacagtat atgggttggg
    74701 tgcaaatgca ttagatgaaa atgcagtaaa gaagatttat gaagctaaag gaagacctag
    74761 tgataatcca ttgatagtcc atatatatga aaaagaagaa gtatatgact tagccaaaga
    74821 tataagtgat aaagctaaac ttgttataga aaagctttgg ccaggaccta taacaattat
    74881 attaaataaa aaagatataa ttccatacaa aactagtgga gggcttgata cagttgcaat
    74941 aaggatgcct tctaatgtta ttgccagggc aataataaaa gaagctggta tacctattgc
    75001 agctccttct gcaaatatct caggtcgccc atctccaaca aaagcaaaac atgtatatga
    75061 agagatgaat ggaagagtgg atggaatagt tttaggagga gacagtaatt ttggattgga
    75121 gtcaactgta ttggatttaa cagaagagac tcctatgata ctgagaccag gaagtataac
    75181 taaagaagtt ttagaggaac ttcttggaga agtaaagctt gacccatcat taagtaaaaa
    75241 agaagataat caaaaagcta aagctcctgg aatgaaatat aaacattatt ctccaaatgc
    75301 agatgtatat ataatatctg gaaaaagaga aaatgtagca acaaagatta atgatatgat
    75361 aaaatctaat agagacaaag gtctaaagac aggtgtaatg tgtattagta gaaaccgaga
    75421 gttttatgat ggagaagtaa tcgatttagg aaactctctt gaagaagttg caagtaatct
    75481 gtttgatgct cttatagaaa tggacaaaaa gggtgttgat gtcatatatt cagaagagtt
    75541 ccccaagact ggagttggac aagcaattat gaatagacta ctgaagtcag caggatacaa
    75601 agtgataaag gcttaagagg gagttgtaaa atatgaatat acttattgtg tgtacaggta
    75661 atacttgtag aagccctatg gctgaggcta tactaaggaa agcaattaaa gaatctggaa
    75721 gaagtattga agaatattct atatcatcag caggtatatc tacagcaaat ggaatgggtg
    75781 cttctgaaaa ttctatagaa gtactaaaag aaatcggtat agacttatca aatcatagaa
    75841 gtaaagtaat tactaaaaaa ttaattgatg aatcagatat aatactaaca atgactaaat
    75901 ctcataaaga aattttagta caagcagttc caaaatgcaa agaaaaagtg tatactttta
    75961 aagggtttgc aaataaaaat gaggaagata tatctgaccc atttggtgga aatttggata
    76021 tttatagaag cactatgaga gaaataatgt attctgtcaa cgaaatagta aaaaaaatat
    76081 gaaacggagg ataaacaaat gaagatagga ttaggctgtg accatggagg atataattta
    76141 aaaaaagaaa taataagcta tttagaaagt aaaggtatag agtgtgtaga ttatggaact
    76201 aataatgcta cagattctgt tgattatcct gtttatggag aaatagttgc aaattcagtt
    76261 ataaataaag aagtagatta tggaatatta tgttgtggta cagggatagg aatatcttta
    76321 gctgctaata aggttcctgg gataagatgt gcagttgtat cagatgtatt ttctgcaaaa
    76381 atgtcaaaag cacataatga tgcaaatatg ttatcattag gcgaaagagt tttaggaaaa
    76441 ggtctagcac ttgagatagt tgaagcatgg attaatacgg attttgaagg tgatagacat
    76501 gctagaagag taaacatgat taaatcaata gaagaaaaac ataataaata ataaaaatta
    76561 cagttttatc aggaggaatt atcaaaatga gcaaagtggt agagacaaac catccattaa
    76621 tacaacataa attaacttta atgagagata aaaatactgg ttctaaagac tttagagagc
    76681 ttttaactga aattgcaatg ttaatggggt atgaaataac aaaagatata ccattaaaag
    76741 atgtagaaat agaaactcct attcaaaaaa catcatctaa agtagttgca ggtaaaaaat
    76801 tagcaataat tccaatttta agagcaggtc ttggaatggt agatggatta gtgagcttaa
    76861 tgccagcagc aaaagttgga catgtaggac tatatagaga cccagaaaca ttaaaaccag
    76921 ttgaatatta ttgtaagctt cctcaagaca taggagaaag agatataata gtagttgacc
    76981 ctatgcttgc aacagggggt tctgctgtag cagctataga tttacttaag tctaaaggag
    77041 ctaaaagtat aaaattagct aatttagtag ctgcaccaga aggtatagca gaagtgcaaa
    77101 aatatcatga tgatgttgat atatatgttg catcagttga cgaaagatta aatgaacatg
    77161 gatatataat tcctggtctt ggtgatgctg gagatagatt gtttggaact aaataaaaat
    77221 aaataactat aaatatttat tagggacttt cttatatagt gtatataatt ttatgtaaga
    77281 aggttctttt ttatatattt tttctcaaaa taggaattat tgaattgata aatttctcga
    77341 attgagaata aaaagttttt accaattaaa aaagattgct tattttacaa ggttttatag
    77401 aatagtagtc tattttaatg tggcatgtta gttgcttacg attataggta ttaagtgtat
    77461 tagttaaatt gagaattgaa tattaataat attattggga gtggtaatac atgaagaatt
    77521 gtatagtagt aagaggggct ggagatttag caacagcagt tattcataaa ttacacaaca
    77581 gtggttttaa agtagttgcg ttagaaatag aagagccatt agctataaga aggcaaattt
    77641 ctttttgtga ggctgtttat gaaaaaaaag taactgttga aggtgtagaa tgtatatttt
    77701 gcaatactga aaaagaaata gaggaagttt tatctcaaca taaagtagcc ttgattatag
    77761 acccaaaagg agaaaaatta tctaaattaa atccaatcgt tttggttgat ggaatactcg
    77821 ctaagaaaaa cttaggtaca actaaagata tggcaccact tacaatagct ttaggtcctg
    77881 gattttgtgc aggagaagat gtagatgttg ttatagagac aatgagaggt cataatctag
    77941 gaagaataat aactaatggt agagcagaaa aaaatacagg agtaccagga gcaataaaag
    78001 gtttttctaa agagagagtt atgcattcac agtatgatgg cataatggaa aatgtatgta
    78061 agataggtga tatagttgaa aagggagacc tactttgcta tattaataat tatgaaaaaa
    78121 aacatgaagt tagagcaaca atatctggag tccttagagg tttattaaag gatggagcaa
    78181 aggtaaacaa aaatttaaaa atactagaca tagaccctag aaaagaagaa gttaagaatg
    78241 cttttacaat atcagataaa gctagatgta tagctggagg ggttttagaa gccatactaa
    78301 acaatgggat ttatccccaa aattaattta tattagataa agctattaat attttattaa
    78361 gatgatataa taaatttaat agattaaaga gagcatatga cgggtattta aaatttaaat
    78421 ggtaaaaatg ctcttttttt attggacatt caattaacat gtgtatataa tataaaaata
    78481 agtatttatt taggttgaaa tctaataaat atataatacc agtacataag caaagaggag
    78541 aaaagtaaat tatgagacca tcatgggatg aatacttcat ggaaattgca gaagtagtga
    78601 aaaagcgttc aacttgtatt agaagacaag taggagctgt aattgtaaga gacaaacaaa
    78661 tattaacaac tggatataat ggttctccga gaaatcttga acactgtgaa aatattggat
    78721 gtaagaggca ggaattaaat attccctcag gagaaaggca tgaattatgt agagcattac
    78781 atgctgaaca aaatgcgata atacaagctg ctcataatgg tattagtgta gatggggcaa
    78841 ctttgtatgt tactacaaga ccttgtgttt tgtgtgcaaa aatgtgcata aatgcaggta
    78901 tattgaaaat tgtttatgaa ggtgattatc cagatgatat gtctacagaa ttattaaaag
    78961 aagcaggaat agaactcata aaattttagt ggataaaatt tatatgacaa taaatgtcaa
    79021 attatgacac tgaaatacaa tggtatcaaa tgattaaaag aaagtatttg aaaaatcatc
    79081 agattagtag taagatatat tttataatta aataaattta tgagatagat taattacatg
    79141 ttatttattt tataataatg agggatacta tttatgagta aaagaaatac acatacagaa
    79201 attgcaaagt tgtttagttt aataagtcaa ataggattga tgattgtaat atcaatatta
    79261 ggatgtactt tcttaggaaa atttttagat agtaaattga atactactcc agttctgaca
    79321 ataatatttt tattgttggg tgttggaggt gcttttagtg gtgtttataa aactctaata
    79381 gtatatacac gaaggaagtg atatattgga tataaaatta cttaaccagg taaggtcagt
    79441 aacaaaaggt atagtaatat ttgatgtagt tttaattgtg ctaatgttga taacatcaac
    79501 attaagtaaa ccagtattat taggacttat atttggctct ataatagcta tgttaaattt
    79561 tagattattg gcgatatcac tggaaaaatc agtgacattt cctgctggga aggcacaagc
    79621 ttatgcatca gcacaatatg ctgttagatt atttataatg gcagttgttc taattgcatc
    79681 agctacagta ccacatttaa atataatcgg aacagcattg ggattattga gcacaaagtt
    79741 tgtcatatta tcaaaaaact ttataaataa attaaagagg aaggaggcat aagtatgaat
    79801 caagcgcatt attatctcat aggaaacttt aagattagtg aaacaatact agtaagttgg
    79861 ggaataatgg cctttttagc gatagcatct tattttttga ctagaaactt aaaaaaagtt
    79921 ccaactagta aagttcaaat attcttagaa tttgcagtag gtggtcttgc taaaatggtt
    79981 acagatgtta tgggtaaaaa tacagtaaaa agaatgccat atatgatacc atacatagga
    80041 agcttgtttt tatttttcgc atgttcaaat ttaagcccat tacttggatt aaggtcacca
    80101 acaaccgact tagacacaac tgctgcatgg gcaatgatta cattttttat gatttatttt
    80161 gcaggagtta aatttaaggg gttatcatac tttaaagaat tggctcaacc acttgccata
    80221 atgttaccaa tcaatattat aggtgagtta gctagaccaa tatcattaag tttccgtcca
    80281 tttgggaata ttttaggtgg aacaataata atgggtctgt tttatcaact tttaggtttt
    80341 atatctagtt taataccagg attatcaata ccaattggag aaatattaat accagtccca
    80401 ttacacttgt actttgactt atttgcagga ttgttacaag catatatatt tataatgtta
    80461 acaatggtat ttatatcaag tgcaggagag ttagattaag aaatcaactt atattaagat
    80521 aaaaataaaa taatttaaaa aattaaagtt ttaaggagga aataattatg gaaacagcaa
    80581 tagtagcggc agcatcagca ataggagcag ggatagcagt tgcaacaggt ataggtgcag
    80641 gtataggtca aggtatagca gcagctaaag ctgcagaagc agtaggaaac caaccagaag
    80701 ctaaaggtga tataacatca actttattac taggagttgc tatagcagag tcttcagcaa
    80761 tatatggttt ggttatatca ataatacttc tattcgtaaa tccattcttt aaatacttag
    80821 gaatgtagat ttagtcctta aatattttga gataggttta taggtaaatc atctcaaact
    80881 tataatcatg agatgaactt tatcatctcg tgattataag tagttatgat ttatggaaag
    80941 gaggataatt atggaaaaag cactagtagg tataacgtgg gaatttgtgt tccaaattgt
    81001 taatactttt ataatatttt tacttctgag aaagctttta ttcaagccag tattaaatat
    81061 aattgaatca agagaaaatg atattaaaag tgatttggca gaaggcgaaa aagcaaaaaa
    81121 tgaaggttta gctctgaaaa aagaatatga gtcaaaaatc aattttgcca aggatgaagg
    81181 tcaagaaatc ataaaacaag caactataag agcagagcag aaatcagatg atatagtaaa
    81241 tactgcaaag aaagatgctc ttgatataaa agaaaaagca aacaaagata tagaacaaga
    81301 aagacaaaag gttataaatg aaatcaaaaa tgatatatct aacatagcac tacttgcagc
    81361 atctaaagtc attgaaaaag atttagataa atctaaacat gaagaattga tagaaaactt
    81421 tataaaagag gtaggggaag ctaaatgata aatgtaatag caaatagata tgcagaagcc
    81481 ctatttcaat taggagaaga agaaaattca actgatgtct tattcaaaga actagaaaaa
    81541 gtagtagata tgatgactaa agttagtaaa gatttctaca aagttttaaa atcaccatta
    81601 gtttcaaaat cagaaaagaa aaacttggta gaaattattt ttagtaaaga agtaagttct
    81661 aacataaaaa actttttaaa agttttagta gataaagata gaatatcata tttagaagat
    81721 atcgaattag cttataaaga attattaaat aagaaaaaca atgtaattga tggagtagca
    81781 ataagtgcta ttccaatgag tgaaacagat attaaagaac ttgaagtcaa actttcaaat
    81841 aaatacaaca aaaatgttac tatagaaaat gttgttgaca agactatact agggggagtt
    81901 ctagttagaa ttggaaatga acaaatagat ggcactgtga aaactcgtct tgataagatg
    81961 aaagaaaagc tatctgaagt aatatcttaa gaatggcggt gaacccatga acttaaaacc
    82021 tgaagaaata agttctataa ttaaacagca aataaaaaat tatgagaata aagttgagtt
    82081 gacagataca ggtagtgttt taacagttgg ggatggtata gcgagtgtat atggattaga
    82141 aaaagctatg tctggtgagt tgctagagtt cccaggtgaa atatatggta tggcacttaa
    82201 cttggaagaa gaagtagttg gagctgttat ccttggagat gacagtgaaa taaaagaagg
    82261 cgatatagtt aagagaactg gaagaatagt tgaagttcca gttggagaag ctttaatagg
    82321 tagagttgta aactcactag gtcagccaat agatggaaaa ggacctatag cgtacacaaa
    82381 aactagacca gttgaatctg aggcacctgg tataatagat agaaggtctg tttatgaacc
    82441 attacaaaca ggaatcaaat caatagattc aatgatacca attggtagag gtcaaagaga
    82501 gttaataata ggtgacagac aaactggtaa aacatctata gttattgaca ctatactaaa
    82561 tcaaaaagga aaagatgtta tctgtatata tgtagcaatt ggacaaaaac gttctactat
    82621 agctcaatta gttagctctt tagaaaaagg tggagctttg gattatacaa tagtagtttc
    82681 agcaacagca tctgaatcag caccacttca atatatagca ccatatgctg gagcagctat
    82741 gggtgaagaa tttatgtaca atgggaaaca tgtacttata gtgtatgatg atttaagtaa
    82801 acaagctgtt gcatatagag aaatgtcatt gttacttaga agaccaccag gacgtgaggc
    82861 atatcctgga gatgtattct atttacattc aagattactt gaaagagcag ctaagttatc
    82921 tgatgaattg ggtggaggtt caatgactgc cttgcctata atagaaactc aagctggtga
    82981 cgtttctgca tatataccaa caaatgttat atctataaca gatggtcaaa tatacttaca
    83041 accagagtta ttctattcag gtgtaagacc agcagttgac cctggtatat cagtatcaag
    83101 ggttggtggt tctgcgcaaa ttaaagcaat gaaaaaagtt gcaggaacat taaaacttgc
    83161 atattcacaa tatagagaac ttgcagcatt ctcacagttt ggttctgact tagatgaaga
    83221 tactaaaaag agacttgctc aaggtgaaag aatcgttgag atattaaaac aaggtgagca
    83281 tcaaccaata aaagttgaaa atcaagttat gataatatat gctgttataa ataatcattt
    83341 agaggatatt ccgatagata atatagctag atttgaatca gaattgtatg catttgtaga
    83401 taataattat ccggaaatat caagaaaaat attaggtgga gaagacttca ctcatgattt
    83461 aacagatgca ataaatgaat ttaaagaaaa gtttgttgtt gaagtataat cctttttaaa
    83521 aaaggaggta aattaattgg caggagctgg aatcaaagca atcaaaacac gtatgggaac
    83581 tgttgaaagt acaaagcaaa taactaaagc aatggaatta gttgcatctt ctaagcttag
    83641 aaaagcaaaa gaaaaggctg aagattcaag agaatatttt aatactttat acaatacagt
    83701 aaaagatatt gcccaaacaa cacgtggaat tagaaatgaa tttttaaaag aaagagaagt
    83761 aaaaaataaa tgctatgtag tagtagctgg agacagagga cttgcaggtg gatacaattc
    83821 taatgtatta aaggaatctg tctctcatat gaaaggtaaa aaggaaacag tgattactgt
    83881 tggaaagaaa gcacatgaat ttttttctaa aagaaactac aatatagcaa aagatataaa
    83941 tagtgttgaa gcttgtggat atgaagatgc gacagaaatt tcacaaacta ttattgacct
    84001 gtataaaaaa ggtgaagtag atgaagttta tattgcttat acaaaattta aatctgcttt
    84061 atcacaagaa gttgaaattt tgaaattgct tccattagtc tttgaaaaag atacagacga
    84121 acaaggaatg gcagtaaaag aagaaaaaca aaatagatca aaagtacaat atataccatc
    84181 agcagaagca gttttaagtt atattgtacc tcagtttata tcaggtacag tatatggtgg
    84241 aattttggaa tcttttgcat cagagcaagg tgctagaaga actgcaatgg aatcagccac
    84301 agacaatgcg aatgaaatgc tatctaattt agagttaagt tataacagag caagacagtc
    84361 agctgtaact caagaaataa cagagatagt tggtggagtt gaagctctaa aatagggagg
    84421 ttaggaaatg gcaaatgtag gtaaagtagt acagattgta ggagctgtac tagatgttaa
    84481 gtttgatagt gaacaaagcc tacctaactt attaaatgcg ttagtaataa agttaggaga
    84541 taaagaaata gttgcagaag ttgctcaaca tataggtgat gatactgtaa gatgtatagc
    84601 tatgagtgca acagacggac ttgtaagagg tatggaagtt gtagatacag gagggccaat
    84661 aagtgttcct gttggagatg aaactttagg tagaatattc aatgttcttg gaaaaccagt
    84721 tgatgggaaa ccagctccaa aatctgcacc taaattacct atacatagac cagcaccagc
    84781 atatgatgaa ctagaaacta cagcagagat actagaaact ggtattaagg tagttgactt
    84841 actagctcca tatctaaaag gtggaaaaat aggtctattt ggaggagcag gagtaggaaa
    84901 gacagttcta atacaagagc ttataaacaa tattgctaag caacatggtg gtatttctgt
    84961 gttttcagga gtaggagaaa gaacaagaga aggtaacgac ctttatggcg aaatgagtga
    85021 gtctggagtt ataaataaaa cagctctagt atttggtcaa atgaatgaac cacctggagc
    85081 gagaatgaga gttgctttaa ctggacttac aatggcagaa cattttagag atgagcaagg
    85141 acaagacgtt ttacttttcg ttgataatat attccgtttc acacaagctg gttcagaagt
    85201 ttcagcactt ctaggacgta tgccatcagc tgttggttat cagccaacat tagctactga
    85261 aatgggtgca cttcaagaga gaataacatc aactaagaaa ggttcaataa catctgttca
    85321 agcagtatat gtaccagcag atgacttaac tgacccagca ccagctacaa cattctctca
    85381 cttagatgca aaaacagttt tatcaagaca aatatcatca ctgggtatat atccagcagt
    85441 tgaccctctt gaatctacat caaggattct tgaccctagt atagtaggaa aagaacatta
    85501 tgaagtagct agaggtgtac aatcaatact tcaaagatat aaagaacttc aagatataat
    85561 tgctatactt ggtatggatg aattatctga tgaagataag ttaatagttg cacgtgctag
    85621 aaagatacaa agattcttat ctcaatcatt cacagttgct gagcaattta caggaaatcc
    85681 aggtcaatat gttcctgtta aagagactgt aagaggattt aaagaaattc ttgaagggaa
    85741 acatgatgac ttaccagagt cagcattctt atttgtagga acaatagaag acgctgttag
    85801 aaaagcgaag ggaagtatgt agtcatggcc agtgaatttt cattgaaagt tgtaactcca
    85861 agtaatactt tttatgatgg agatgttgaa atgatagtag ttagaactac aaaaggagac
    85921 aaaggggtgt tgaaaggtca tataccattt gtttcaggta tacctgtagg aactttaaag
    85981 ataaaaaaag agggaagttt taaagaggcg aaaatagcag aaggattcat taatgtaact
    86041 caagataaaa ctgtcatatt gacagaatct gctgagtgga tttaataaat taatttagag
    86101 tataaaaagt cattttgacc aaaaaaactt ttgggtaaaa tggctttttt gcgttttggt
    86161 gttaataaaa taacgataaa attgtaagtt tttagtgata ataaattcac aaaatgttta
    86221 aatttactgc caaattatgt tgtaattaat aagataaaga agatttagat agcttttatg
    86281 taagtgaaaa agtcttcata taagaaatac atataaaatt tttataaaat ttaaaaaatt
    86341 tgcttagttg tgtag
//
LOCUS       DAMJYR010000003        77287 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.003, whole genome shotgun sequence.
ACCESSION   DAMJYR010000003 DAMJYR010000000
VERSION     DAMJYR010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 77287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 77287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..77287
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.003"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(121..894)
                     /locus_tag="PC471_000194"
     CDS             complement(121..894)
                     /locus_tag="PC471_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SigB/SigF/SigG family RNA polymerase sigma
                     factor"
                     /protein_id="HDF2787342.1"
                     /translation="MKNVANATHYLNMDTKELFNLYNKNKNVDIRNILIERHLYLARL
                     LAKKYINKGVDFEDIYQVASLALIYAIDRYDVEKGFEFSSFATPTIVGEIKKYFRDKV
                     WTLRVPRRIQELSKKISDAKIKLEQENKKHPKVKDIADYIGVSEEDVLEAMEASYGYQ
                     PMSLDSSSNDDSEDKDITLIDKIGKEEGNFGSIEYEDFINKFIETLNELEVKIFKDRF
                     FFDKTQSSIAKELGISQMTVSRLEKKVVEKLKKEYEKNL"
     gene            complement(894..1280)
                     /locus_tag="PC471_000195"
     CDS             complement(894..1280)
                     /locus_tag="PC471_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDF2787343.1"
                     /translation="MEITTNPDYVSIIRLTASGIANKMGFPIDDIEDIKVAVSEACTN
                     AIKHSKDDVFNIVFNILDNAINIEIQDNGKGYDVSSISTPDLENPKESGLGLFIIKTL
                     MDDVDIESEHNQGTKIKMTKYLGVDI"
     gene            complement(1305..1622)
                     /locus_tag="PC471_000196"
     CDS             complement(1305..1622)
                     /locus_tag="PC471_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HDF2787344.1"
                     /translation="MSMNIDSNLDSQNKFWNVCLDGELDVSTADKLKEHLHALIEKNM
                     LDVKINLKDLDYIDSTGLGAMIGVLKKLKINEKEIYIVNPKSNVRKIFTITGLDKIFK
                     VEG"
     gene            complement(1634..1894)
                     /locus_tag="PC471_000197"
     CDS             complement(1634..1894)
                     /locus_tag="PC471_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HDF2787345.1"
                     /translation="MNAWGWQIGAVLVGSSALIVAIYLAKTLNSINKVVEKAYKIVDY
                     NERHIQDIVENASSISKGIDDIVYVINKILSIGNIFKIIKRK"
     gene            complement(1894..2190)
                     /locus_tag="PC471_000198"
     CDS             complement(1894..2190)
                     /locus_tag="PC471_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HDF2787346.1"
                     /translation="MCNKRSCKKGGFLLLGAILGFIFGMFFAPKKGSELRKETKEKFN
                     DVKENPKEVLHETFNDVKERIINLVDDDNNEEDIKISEEDIVISKSFDDEGDVN"
     gene            complement(2259..4685)
                     /gene="gyrA"
                     /locus_tag="PC471_000199"
     CDS             complement(2259..4685)
                     /gene="gyrA"
                     /locus_tag="PC471_000199"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HDF2787347.1"
                     /translation="MEENNKILPIEIAEEMKKSYIDYSMSVIAGRALPDVRDGLKPVH
                     RRILYSMSELNLTPDKPYRKSARIVGDVLGKYHPHGDTAVYYAMVRMAQDFSTRALLV
                     DGHGNFGSVDGDSPAAMRYTEAKMSKLSLELLRDIEKETVDFKPNFDESLKEPSVLPA
                     RYPNLLVNGSNGIAVGMATSIPPHNLAEVIDATVYLIDNPECSVDDLIKFVQGPDFPT
                     AAIIMGKESIAEAYRTGRGKVKVRSRAFIEELPKGKQQIIVTEIPYQVNKAKLVERIA
                     ELVKEKRIEGISDLRDESNRNGMRIVIELKRDANANIVLNNLYKHSQMEDTFSIIMLA
                     LVDGQPRVLNLKQILYHYIKHQEDVVTRRTKFELNKAEARAHILEGLKIALDNIDAVI
                     SLIRASKTGQEAKLGLIEKFKLTEIQAQAILDMRLQRLTGLERDKIEAEYEDLIKKIN
                     RLKEILADERLLLNVIKDEITIIKENYSDERRTEIRHAEGEIDMRDLISDEEIAITLT
                     HFGYIKRLPSDTYKSQKRGGRGISALTTREEDFVRHLVTTTTHSRLLFFTNKGRVFKL
                     NAYEIPEGKRQAKGTAIVNLLQLSADEKIATLIPIDGNDENEYLLLATKKGIVKKTKR
                     EEFKNINKSGLIAIGLRDDDELIGVELTDGKQEVLLVTKEGMSIRFDENDIRYMGRTA
                     MGVKGITLSKEDFVVSMNLCSKGTDVLVVSKNGFGKRTNIEEYRSQIRAGKGIKTYNI
                     SEKTGTIVGADMVNEDDEIMIINSDGVLIRIRVNEISLFGRVTSGVKLMKTNDEVNVV
                     SIAKINIEEE"
     gene            complement(4705..6606)
                     /gene="gyrB"
                     /locus_tag="PC471_000200"
     CDS             complement(4705..6606)
                     /gene="gyrB"
                     /locus_tag="PC471_000200"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003226808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HDF2787348.1"
                     /translation="MKQEYGASQIQVLEGLEAVRKRPGMYIGSTSPRGLHHLVYEVVD
                     NSIDEALQGYCSDIYVSINEDGSVLVKDNGRGIPVEIHPKTGKSTLETVLTNLHAGGK
                     FGGGGYKVSGGLHGVGVSVVNALSKWMVAEVYLNGKIYKQTYEKGLPTSKLEVVGESQ
                     DKGTMIQFMPDETIFDEIEFKYETLEYRLRELSFLNKGIKIVFEDKREGQEKRKEFHY
                     TGGLVEYIKYLNKSRTGIHDDIVYIDKKVDDCFVELAMQYTDGYTENIYSFANNINTH
                     EGGSHLSGFKAALTKTVNDYAKRNKFLKENDVNLLGEDIREGLTAVVSVKLPEPQFEG
                     QTKTKLGNSFMRGIVDSVTVDELGSFLEENPSTARIIVDKALRAQRAREAAKKARELT
                     RRKSVLESTSLPGKLADCAEKDPSKSEIFLVEGDSAGGSAKQGRDRNSQAILPLRGKI
                     LNVEKSRLDRILSSDEIKNMITAYGCGIGEDFDIDKARYHKIIIMTDADVDGAHIRTL
                     LLTFFFRYMRPLIDEGYVYAAQPPLYKVTKQKKEHYVYSDKELNILLDEIGRNGVELQ
                     RYKGLGEMNAEQLWETTMNPETRTLLQVTVEDAAIADEVFSMLMGDKVAPRKEFIEEN
                     ARFVRNLDI"
     gene            complement(6607..7722)
                     /gene="recF"
                     /locus_tag="PC471_000201"
     CDS             complement(6607..7722)
                     /gene="recF"
                     /locus_tag="PC471_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HDF2787349.1"
                     /translation="MKLKSLQLVNFRNYKKLHLEFNGKVNLLVGKNGQGKTNIVESIY
                     MLSFGKSFRTNKDKEMVRFNSENLYIGGSFSKYNKYSLIELIIGKDKKGIRINKVPLQ
                     KIQELLGNLNVVIFSPEDLRLVKEGPKERRAFIDKEISQIIPKYYKYLTNYNKTLSQR
                     SRVLKNIHVDEALLDVYDDTLAKYGSYIYILRRDFIKKIANISENMHMNLTNGVERLS
                     IRYKNQINITDEDTIDTVYNKFLAKLSSNRPNDIESKTTRYGIHKDDLNIFINDLDAR
                     LFGSQGQQRTASISLKLSEIELIKNEVEEYPVLILDDVFSELDEARQKLLVNNLSNVQ
                     MFITSAEVSHKKIFDEKNVTIFNIENGDVISIENGGN"
     gene            complement(7740..7946)
                     /gene="yaaA"
                     /locus_tag="PC471_000202"
     CDS             complement(7740..7946)
                     /gene="yaaA"
                     /locus_tag="PC471_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HDF2787350.1"
                     /translation="MTEITIESEYIKLDQFLKLAEIASTGGHAKFLIQEGLVTVNDEI
                     ELRRGKKIKSGDIVEIEGTKIKVL"
     gene            complement(8083..9189)
                     /locus_tag="PC471_000203"
     CDS             complement(8083..9189)
                     /locus_tag="PC471_000203"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HDF2787351.1"
                     /translation="MKIICNQKILANRIGIAQKAINGKTTIELLKGILISTEEGQLKL
                     TGYDAEIGIETYVQAEIIEKGDVVVDARLFGDIIRKLPDSFVEIETDSENNIYINCVN
                     SRFKIKGYAAKEFPKLPELNEEDLYSIPQEILKNMIKQTVFAISQDQTKPVLMGELLE
                     IVDRNLNLVAIDGYRLAVKSCSVDSLTENIKVIIPGKTLIDVNSLLSGEDNVKVGFNE
                     KNAIFIINDTKIITRLLEGDFIDYKKLLPREHNSRVKLNTKELLNSIERASLLSQSEK
                     NNLIKLSIRDKVMAITSNTEKGNVYEEVEIDLDGDYLDIAFNSRYFIEGLKNIDNEEI
                     FIEFTTNVNPCIIKPTDDVNYIYLLLPVRISSNI"
     gene            complement(9431..10753)
                     /gene="dnaA"
                     /locus_tag="PC471_000204"
     CDS             complement(9431..10753)
                     /gene="dnaA"
                     /locus_tag="PC471_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HDF2787352.1"
                     /translation="MMDIVSLWDKTLQLIKGDLTSVSFNTFFKNIVPLKIHLNDLILL
                     APSDFNKDILENRYLHLIEDAISQLSLKKYNIKFVLSEKEVADLNSDSTDLNYRVLYP
                     NLNPKYTFDTFVIGNSNRFAHAACVAVAESPAKAYNPLFLYGGVGLGKTHLMHAIGHH
                     IVSQKKDSKVVYVSSEKFTNELINSIKDDKNEEFRNKYRNVDVLLIDDIQFIAGKERT
                     QEEFFHTFNTLHEANKQIIISSDRPPKDIPTLEDRLRSRFEMGLITDIQAPDFETRIA
                     ILRKKAQLERIDVPNEVMSYIAKNIKSNIRELEGALTRVVAYSSLSNRVISFDLATEA
                     LKDIITTSKNEEINVLRIKEKVSSVFNLKMEDFNSKKRTRSIAYPRQIAMYLTRELTD
                     LSLPKIGEEFGGRDHTTVIHAHDKVSKDIEESEEIKTKIDKIISDLKG"
     gene            11409..11546
                     /gene="rpmH"
                     /locus_tag="PC471_000205"
     CDS             11409..11546
                     /gene="rpmH"
                     /locus_tag="PC471_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HDF2787353.1"
                     /translation="MSKRTYQPKKRQRSKEHGFRKRMKTSNGRNVLKRRRAKGRNRLT
                     H"
     gene            11624..11968
                     /gene="rnpA"
                     /locus_tag="PC471_000206"
     CDS             11624..11968
                     /gene="rnpA"
                     /locus_tag="PC471_000206"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HDF2787354.1"
                     /translation="MDFNRTKGLKKDSDFRKVYKHGKSFANKYLVIYILKNKSDYSRV
                     GISVSKKVGKAITRNRVRRLIKEAYRLNIDEKIKPGYDIVFIARVSSKDATFKDIDKS
                     IKNLVKRTDISI"
     gene            12000..12251
                     /gene="yidD"
                     /locus_tag="PC471_000207"
     CDS             12000..12251
                     /gene="yidD"
                     /locus_tag="PC471_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HDF2787355.1"
                     /translation="MNKIIYILKEISKYLSNLCIYLVRFYQKYISPLKGPTCRFYPTC
                     SQYSIEAFKKYGFVKGMYLTIRRILKCHPFHPGGYDPLK"
     gene            12283..12990
                     /locus_tag="PC471_000208"
     CDS             12283..12990
                     /locus_tag="PC471_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HDF2787356.1"
                     /translation="MNIIGNFLGLILKVIFEFVNHYGISIILFTILVKIILLPLTIKQ
                     TKSTKAMQDIQPRIKEIQEKYKNKPEKQNEEIVKLYGEAKINPLSGCLPLLIQFPILI
                     GLFSVLREPVAHGVFANKAAFLAADNGFLWIKSLTSPDYVLAVFSGASAYVMQKVMTP
                     KDQLQGSMKVMTYVMAGMSFYWGFIFPAGLTLYWTVSNLFSIAQYYLIMNPLKAKLAA
                     NSKEEKVNEKKPKINKK"
     gene            12959..13588
                     /locus_tag="PC471_000209"
     CDS             12959..13588
                     /locus_tag="PC471_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein jag"
                     /protein_id="HDF2787357.1"
                     /translation="MRKSLKLIKSKSKEEAISKAIAELNLKAEDIEVEILENPSKGFL
                     GFIGAKDGTYEIFVIEREVDVAKNFIEVMLKNANVDAKVNVSQKDNLIMVDIEGKEAA
                     SLIGRRGETLDSIQFLTGLALNKINKDSHTRVLVDIENYRSKREESLIRYANKVAREV
                     AKTRKTKKLDYMNPYERRIIHSALQNDKYVITYSEGTDPYRRLVIECKR"
     gene            13700..15079
                     /gene="mnmE"
                     /locus_tag="PC471_000210"
     CDS             13700..15079
                     /gene="mnmE"
                     /locus_tag="PC471_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HDF2787358.1"
                     /translation="MFIDDTIAAIATAPGEGGIGILRISGEKALKVAEEIFKSMSGKS
                     IEEYNKRTLIYGNIVDNENIIDEVLLAYMKGPNSYTGEDVIEINCHGGFISVKKILEL
                     ILSKDVRLAEAGEFTKRAFLNGRIDLSQAEAVIDVIKAKTDIAHEVAQNQLEGSLSKK
                     IRELRDKVTEILAHVEVAIDYPEEDIEHITYQTLKEKTDELKKDIKKLYDTAESGKIL
                     REGLKTVIVGKPNVGKSSLLNSILGENRAIVTDIPGTTRDVIEEFVNIKGIPLKIVDT
                     AGIRDTDDIVEKIGVEKSKESFTSADLIVMVLDASRKLSEEDIEILEKLKDKQTIVLL
                     NKNDLKQEIEEEKILKYVENNSIIKISALQQEGIEELQDKIESMVYKGSIKNNSSLVV
                     TNSRHKDALSKAYKSATDALIALEQSMPFDFVEVDLKNIWDYLGYINGDTVTEDLLDN
                     IFHNFCIGK"
     gene            15177..17069
                     /gene="mnmG"
                     /locus_tag="PC471_000211"
     CDS             15177..17069
                     /gene="mnmG"
                     /locus_tag="PC471_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012065747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HDF2787359.1"
                     /translation="MKFEAGKYDVIVVGAGHAGCEAALATARMGYKTLIITMSLDSIA
                     LMPCNPSIGGTGKGQLVKEIDALGGQMGLNIDKTYIQSRMLNTAKGPAVHSLRAQADK
                     FKYHEEMKKTLEDEPNLDIAMDEVVEILHEGNVVIGVGTKLGCSFKSKAVILATGVYL
                     NSKIYMGEVAFYEGPNALGYAKYLTDSLVELGLRMRRFKTGTPARVHRDSIDFSVMSL
                     QEGDEKVTPFSFMNENIEKKQEPCYLTRTTEETQKVILDNLKRSAMYSGVIESTGPRY
                     CPSIEDKVVRFSDKTSHQLFIEPEGLNTKEMYIQGISTSLPFEVQLDMYKTIKGLENC
                     KIMRPAYAIEYDCVDPTQLKISLEIKGVENLFSAGQFNGTSGYEEAAAQGLMAGINAV
                     RKIEGKEPFVLDRSEAYIGVLLDDLVTKGTNEPYRMMTSRAEYRLYLRQDNADMRLTQ
                     KGYDIGLVKKDRYERFLNKKAAVEKEFERLKNERVTPKEVNSLLEEKGATPIKVGISL
                     YEFLKRPEVTYELLEELGKGAGEDVSREVKEQCVIITKYEGYIEKQLKQIDQFKKLEN
                     KKLDEKINYSSIEGLRLEARQKLDDIKPISIGQASRISGVSPADISVLLIYLEQIRRT
                     RGGKGE"
     gene            17062..17781
                     /gene="rsmG"
                     /locus_tag="PC471_000212"
     CDS             17062..17781
                     /gene="rsmG"
                     /locus_tag="PC471_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HDF2787360.1"
                     /translation="MNNIELLKNGIEGFNIDTNDSMLEKFKIYREILVDWNKKMNLTG
                     IEDEKEVYIKHFLDSISAVKNGYIKNGMSIIDVGTGAGFPGIPLKICLDSLELTLLDS
                     LNKRINFLEEVSRVLELDNITFIHGRAEDFGKDEDYREKYDVATARAVAGLPILMEFC
                     VPFVKVGGYFICLKGPNANLELEESQKAIDVLGLEYIEKIDVELPEVDLNHNILVFKK
                     VMETPVKYPRKAGKPAKNPIK"
     gene            17944..18729
                     /gene="noc"
                     /locus_tag="PC471_000213"
     CDS             17944..18729
                     /gene="noc"
                     /locus_tag="PC471_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion protein"
                     /protein_id="HDF2787361.1"
                     /translation="MEDKRVMEIPIEDVVPNPYQPRKIFSQVSLEELSNSIKVYGIIQ
                     PITVRAKDGKYELIAGERRLRAAKLAELKTIPAIINNMNDESSAVLALLENLQREDLN
                     FIEEAIGYENLIKEHAFTQQQLAEKLGKNQSTVANKLRILKLPNDIKMKLIENNLTER
                     HARAFLKLPSEDLMQSVLDKVIKNELTVKKTEKLIQDVLEETKVQEEPDKKQNIKGAM
                     SIRIYINTIKQAFDAISNTGIDAKYNEIDKGDYMEVVVKIPKK"
     gene            18864..19637
                     /locus_tag="PC471_000214"
     CDS             18864..19637
                     /locus_tag="PC471_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HDF2787362.1"
                     /translation="MGKVIAVFNQKGGVGKTTTNVNLSASLGTLGKKILVLDLDPQGN
                     TTSGYGINKNEVENTIYEIMLDGLHIKEAIISTEFENVDVVPSATELSGAEIELTSKT
                     NREYILKNSIKAVIDEYDYIFLDCPPSLGMLTINCLTAVDSVLIPIQCEYYALEGVSQ
                     LMETIKLVKSRLNADIEIQGVVLSMFDGRANLSIQVVEEVKKYFKGSVYTTLIPRNVR
                     LAEAPSHGKPVIYYDKRCRGSVAYLELAEEFIDLEEEEW"
     gene            19639..20502
                     /locus_tag="PC471_000215"
     CDS             19639..20502
                     /locus_tag="PC471_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HDF2787363.1"
                     /translation="MENKSKRSNRLGRGLSALIPEIKGETSEKEIVNIDIDKIYPNEV
                     QPRKQFDEEKIKVLSDSIKNYGVLQPIVVKMDENNKYMIIAGERRFRASKLANKNQIP
                     AIIKDIDMKDIMEIALIENLQREDLNSIEEALAYKSLIEHYNVTQEEISEAVGKSRPH
                     ITNTLRLLNLGQDVIEMIDSGRITAGHGKALLRIADKDLQLQIAKKIEEEELSVREVE
                     NIAKKISENKQEEPKKSKPKDVFILDVEDKLRNIFGTKVNISKGKKKGKIEIEYYNDD
                     DLNSIVSMLLE"
     gene            20535..21677
                     /locus_tag="PC471_000216"
     CDS             20535..21677
                     /locus_tag="PC471_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HDF2787364.1"
                     /translation="MIYLDNAATTYPKPERVYNAVLDCMKNYCANPGRAGHKLAMRAA
                     REIYDTRENIAKLFNVSNPMNIVFTSNATDSLNLAIKGVLQEGDHVITTSMEHNSVIR
                     PIKALEKRGIENTVVKCDYEGFLDYEDLEKSIKSNTKLIVTTHASNVCGTLIDIKKVG
                     EIAKKHNILFLVDASQTAGVYDIDVNECNIDMLAMPGHKCLFGPQGTGILYVREGLNL
                     NILKEGGTGSKSEEIVQPELFPDKYESGTHNTPGIAGLNQGILFIFERGINNIRQHEE
                     ELCQYMIDKLEEVPDIKIYGPKDSKKRASVIALNIGDMDSGEVTFLLDSDYNIATRSG
                     IHCSPLAHTTLGTLKQGAVRFSIGYFNTKDEIDKAVEALKKISKNK"
     gene            complement(21811..22386)
                     /locus_tag="PC471_000217"
     CDS             complement(21811..22386)
                     /locus_tag="PC471_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GerMN domain-containing protein"
                     /protein_id="HDF2787365.1"
                     /translation="MKNKKIMLVLSILSISIFAVGCTNAQNGSDSSKKETKSNTNVEQ
                     PKEENNTEKEVPNNKAKPTPKEETKTQSATIYSFDVDKTDLIENKVDLNKIDENTLFE
                     ELQKLKVVPESAKLNSFTTKDIDGVKTGILDVSSDFTKSNLGSDAETLMLDSVARTYI
                     KNMNVEQIKITVDGSNYESGHIVLEEGDYLK"
     gene            22646..23938
                     /locus_tag="PC471_000218"
     CDS             22646..23938
                     /locus_tag="PC471_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HDF2787366.1"
                     /translation="MEILSLGEKIKKLRKEKNMTLKELAGDRITAAQISHIERDKSHT
                     SYELLEYLGDKLDVSVEYLLETKEMQSKKITDNLILQSEIYIKENELNKAEDQIKEVL
                     DICKRYDLMENYGRCNFLMANINLKKSNYNDAIENFEKALYFFIKNSDSENILKCYLN
                     IGKIYMQEDFYKGAISHFNFAEEILSENKFEDVNIHKELYSKMAYCYIKLDNPNMSLK
                     YIEKINEIDNRNDRKEDVDILVLKANNMLEIGKYEESRDYFKKALKILESEDNKTGLA
                     NVYLTICDVYRKIGETDRVLEYSQKVYDIKKNDEDEYMMSGLFKIIEAYIDKEDYDLA
                     RKYCKIALASSIKNKNKFNEYKALRFYANMYKNQNEVTLAIEYLIKCIKIVSDLGNNR
                     ILANLYIDLGQLYSNISKEKELEYYQKGVFMYKNLEIM"
     gene            24043..24645
                     /gene="yedF"
                     /locus_tag="PC471_000219"
     CDS             24043..24645
                     /gene="yedF"
                     /locus_tag="PC471_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HDF2787367.1"
                     /translation="MSIKIDARGLACPKPVINTKRELDNLEEGVVVTIVDNETAKENI
                     LKLAKSLSCEANIVDEKENFISIEIRKGKNILDKQNVVDNKESELDNTCIFISSDKMG
                     LGNDELGKVLIKGFIYTLTESKPYPKYVLLVNGGVKLSAENEETIENLKILENMGVEV
                     LSCGTCLDYYNLKDKLQVGTVTNMYTIVETLKNASNTISI"
     gene            24718..24981
                     /locus_tag="PC471_000220"
     CDS             24718..24981
                     /locus_tag="PC471_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3343 domain-containing protein"
                     /protein_id="HDF2787368.1"
                     /translation="MNQMYIVSFNSTHHAIRSEKLFGENSLKVMALPTPREITASCGI
                     SIKFSFEDMEKIKTILVENNVDIKGIYCISKLENGSKEVEKLD"
     gene            24996..25181
                     /locus_tag="PC471_000221"
     CDS             24996..25181
                     /locus_tag="PC471_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HDF2787369.1"
                     /translation="MPMDLKIGDIVELKKQHACGCKEFEIIRTGMDIKIKCTKCSRLI
                     MLDRETLEKRVKKLIKK"
     gene            25284..26480
                     /locus_tag="PC471_000222"
     CDS             25284..26480
                     /locus_tag="PC471_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HDF2787370.1"
                     /translation="MAVKYAKRMQGLQGSEIRELLKLTQQPQIISFAGGMPAPELFPV
                     EEMKKVSVAVLEENGRSAMQYTTTEGYEPLREKIAARMNDKNKTNVNKDDILVTSGSQ
                     QGLDFAGKVFIDEGDVILCESPSYIGAINAFKSYQPKFIDVPTDSDGMIMEELEKILE
                     TTDRIKMIYVIPDFQNPTGRTWPLERRKKFMEIVNKFEIPVIEDNPYGDLRFEGETLP
                     SLKSMDTKGLVIFLGTFSKIFCPGYRLGWTCASPEILSKFNFAKQGADLQASTISQME
                     VSKFMDMYDLDAHVDKIKAVYVKRRDVMLKTMEEEFPEGLVFTHPEGGLFTWVELPSN
                     LNAKELMPKCLDKNVAYVAGGGFFPNGGRENTFRLNYSNMPEEKIIEGIKNIAAVLKE
                     AMGVEA"
     gene            26694..26972
                     /locus_tag="PC471_000223"
     CDS             26694..26972
                     /locus_tag="PC471_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HDF2787371.1"
                     /translation="MRNYELVYVVKPNSDEEVREAILNKVKEVVATDGEIVKVDTWGT
                     KKLAYPIAKFTEGFYVLVNFKSAVDVPKEIDRNLKINENVIRHMIVVA"
     gene            27009..27443
                     /gene="ssb"
                     /locus_tag="PC471_000224"
     CDS             27009..27443
                     /gene="ssb"
                     /locus_tag="PC471_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HDF2787372.1"
                     /translation="MNQVVLVGRLTRDPELRYIPGTGTPVASFTIAIDRDYVKKDGSK
                     ETDFIPVEVMGKSAEFCANYITKGRLVALQGSIRVDNYQTQSGEKRTFTKVSTRSVQA
                     LDSKNKSENSYRESAPAFEPSFEPQGLDPQGFQAIDDDDIPF"
     gene            27462..27689
                     /locus_tag="PC471_000225"
     CDS             27462..27689
                     /locus_tag="PC471_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HDF2787373.1"
                     /translation="MMNKKRRKKKRVCQFCADKNAKIDYKSTQRLQKYITERGKILPR
                     RISGTCAKHQRELTVAIKRARNIALLPYTLD"
     gene            27832..28152
                     /locus_tag="PC471_000226"
     CDS             27832..28152
                     /locus_tag="PC471_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HDF2787374.1"
                     /translation="MKIDKGSEITRNIKIIEWMKTEILMSVSDLFNLLFKGVKPLDEA
                     IQDTLANIIMITYLLAKRLGISFRDVDYKVKEKIKIGIDEDHSVERWYGDLSNLKKHM
                     DNRE"
     gene            28160..29116
                     /locus_tag="PC471_000227"
     CDS             28160..29116
                     /locus_tag="PC471_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YybS family protein"
                     /protein_id="HDF2787375.1"
                     /translation="MNNKIRLSKAMSIIVLAIIIALVIAYIPMLGIFSMLAAVPYVII
                     GAITDRKYSLISILVTFCVLILFADISYALNICIMYSLPGIVIGKMLKRSFEQEDSNK
                     FEPIYGGTIIFVLSMLVYFFVLKTFLNVNLLDEVSKMISEIVNIQKNSFSATELKVFD
                     KMKPDEIVSYFTNMLPMMLFLQGLLSAFVTYYLSVFFIKRITKMNIRFPKFADFYLPG
                     NAILTTFLLYLLVLFIQIIGSKLYTELIMTNLQLVFNLMFVIQGIAVCIYFLKKWIRQ
                     GPNKIMFFSGIILCLFGFMGISFVGMVDSIIDFRKVRSYKST"
     gene            29127..31121
                     /locus_tag="PC471_000228"
     CDS             29127..31121
                     /locus_tag="PC471_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HDF2787376.1"
                     /translation="MSNKQTFKLNMPEINLYIIVIGISSIILLYYNLYVGCLFFCIFV
                     YMVFHNWRTTNIRRHEWTEYIQNLSLDIDETTKKAIINLPIPLCILEFDGNISWYNGK
                     FYDMIGQKDLLDKNIEDIVKNLNLRKVLNENKEMYTEINYKEKEYTIIYNVIKNDQEK
                     NPKYLMILYWIDKTEYLKVKQNYDDEKNAMMLIQVDGYDEVLKSAAEDKRALINVEVE
                     KILSALELNSNGALRRTSKDKFFLVMHKKELKKLEAEKFSILDTIRHIDYGNNLPVTI
                     SIGIGIDGDTLNENLKLATGALDLALGRGGDQAVVKTKDKFVFYGGKSKAVEKKTKVK
                     SRLIGHALREVIQQSDQVYIMGHKYPDMDAMGAAVGVYDICKSCNKTANIVLQSVNES
                     IEIFINKINENNYYKKLFIGKEEAIDNCTKNTLVVVVDTHRPNYTECEELLKLSEKVV
                     VIDHHRRGVEFINDAVLLFHEIYVSSTCEMVTELVQYMDEDVTINKLTAEGLLAGISL
                     DTKNFAFKTGVRTFEAASYLRKVGADTIEVKKFFNSDVKDFIIKAEIIQSTKIINNRI
                     CLAYSSTEIDSINVIIAQTADELLNIKEVEASFVLGEKDDTIFISARSLGQINVHVLM
                     EKLGGGGHIDIAGAQLKNVSLKEAYKMVNKIIEEYLEEEE"
     gene            31122..31571
                     /locus_tag="PC471_000229"
     CDS             31122..31571
                     /locus_tag="PC471_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HDF2787377.1"
                     /translation="MKVILLKDVKGTGKKGEMKEVSDGYARNFLFPKKMAVQADSVAI
                     KELNEKNKSKEIKAQKEYEEAVLLGKQMEEINIEIYSKAGEGGRLFGSITSKEIAEQL
                     KKQKDIDVDKRKILLDEPIRSLGSTFVEIKIHQKVTTKIRVDVKEKQ"
     gene            31590..32918
                     /gene="dnaB"
                     /locus_tag="PC471_000230"
     CDS             31590..32918
                     /gene="dnaB"
                     /locus_tag="PC471_000230"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HDF2787378.1"
                     /translation="MEDMTRIPPHSVESEQSILGSILLDKDAIITVTETIKPDDFYKE
                     AHKIIYECMITLSNKGEPIDLITLTEELRKQGHLNDIGGISYITSLSTIVPTTSNVKY
                     YADIVKEKSVLRKLIKASNEIINLGYSGATKIEDVLEQAEKSIFDISQEKTSDDFKSI
                     NLVLMDAYDMIEKLYTNKSDVTGITTGFKDLNKKINGLQRTDLILIAARPAMGKTAFS
                     LNLVQNAALKGDASVAVFSLEMSKEQLVQRMLSSQSSVELKKIKTGTLNDNDWPRIID
                     AMAVLSDAKIHIDDTPGIKISELRSKCRKLKIEKGLDLVLIDYLQLMEGEGNNESRQQ
                     EISKISRSLKILAKELNCPVVALSQLSRAPEQRADHRPMLSDLRESGAIEQDADIVMF
                     LYRDEYYHADSESKNIGEVIIAKNRHGETGSVELVWLGEVQRFGDKLRDL"
     gene            33132..33440
                     /locus_tag="PC471_000231"
     CDS             33132..33440
                     /locus_tag="PC471_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2787379.1"
                     /translation="MEKISLKYIYPNIIKVLDEINLFRVIDNNLRESIVVYANNVDNQ
                     YHINMTNTNFGNIINICKLEKLLDVDKFMEKVIKYEKEIIEKEEFSKIEEYMLNIGEY
                     "
     gene            33522..34811
                     /locus_tag="PC471_000232"
     CDS             33522..34811
                     /locus_tag="PC471_000232"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HDF2787380.1"
                     /translation="MKTVAIVGSQWGDEGKGKVIDYLATQADVVVRGQGGNNAGHTLV
                     VEGKKYALHLIPSGVLNPNTVNIIGNGIVFDPKGFLEELEMFKTDNISTENIKISDRA
                     HVIFPYHKELDALSEEARGDLKIGTTKKGIGPCYMDKTERSGIRICDLMDKDKFAIKL
                     KAQIDAKNEIVKNIYGKEELFDFETIYNEYLGYAEQIRKYVADTSVIVYDAVRAGKKV
                     LFEGAQGTLLDLDLGTYPFVTSSHPTSGGFAIGAGIGPNMIKDVVGIVKAYTTRVGEG
                     PFVTEQINETGDKIREQGHEFGVTTGRPRRCGWFDAVIVKYAARVNGLTSISFMLLDV
                     LTGFDKIKVCTSYKMGDKIITDFPASLDDLAKCEPVYEELDGWNEDITQIDNFDDLPE
                     NAKKYVAKIEELVGVSVDMVSVGPNRAQTIIRRNIFA"
     gene            35137..35212
                     /locus_tag="PC471_000233"
     tRNA            35137..35212
                     /locus_tag="PC471_000233"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:35170..35172,aa:Lys,seq:ttt)
     gene            35219..35293
                     /locus_tag="PC471_000234"
     tRNA            35219..35293
                     /locus_tag="PC471_000234"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:35252..35254,aa:Glu,seq:ttc)
     gene            35298..35373
                     /locus_tag="PC471_000235"
     tRNA            35298..35373
                     /locus_tag="PC471_000235"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:35331..35333,aa:Val,seq:tac)
     gene            35407..35481
                     /locus_tag="PC471_000236"
     tRNA            35407..35481
                     /locus_tag="PC471_000236"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:35439..35441,aa:Thr,seq:tgt)
     gene            complement(35864..36856)
                     /locus_tag="PC471_000237"
     CDS             complement(35864..36856)
                     /locus_tag="PC471_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDF2787381.1"
                     /translation="MNEDKIRKILAKYAKRRDDNELLLEHRKNEVYNRIPEIKSIDDE
                     ISKIGLSLAKIVLLNPKSKDEIVKKTKENIESLKVKKERLLAESNIPLDYLEIKFQCI
                     SCKDKGFLPNGEKCSCLKQEIVNEAYKMSNLDRILSQENFSNFNLNIFSPKKGSDGEI
                     SPRENMLNNLSICENFVHDFKKDNSENLLFYGSTGLGKTYMCNCIAKELLDKGNVVIY
                     QTSFRILDILEDYKFRRDTNNQISEDNYKNLFDCDLLIIDDLGTELNNSFTSGEIFNI
                     VNTRLVAGKKIIISTNLTPSQIGNTYTQRTLSRILDKFRILEFTGDDLRWERFK"
     gene            complement(36865..37923)
                     /locus_tag="PC471_000238"
     CDS             complement(36865..37923)
                     /locus_tag="PC471_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HDF2787382.1"
                     /translation="MFFKESNEIDLGEITIPNIFIDIFMPMADGLYVKVYLLGYRQAC
                     DITSNPKFDNNSIAKNLNIPLSDVLSAWKFWEEKKIIKIHDNEEYDNFNYSIEFLDLK
                     NFYIENILSNNSSIKSNTDKVVSTSENPSIRKMFNSINKIVGRYLDPSEKMSIMDIMN
                     KYNMSPDMILCAYEYVKDKTGTSKPVKYIEGIIRNWYDSNLYTPKDVEESFLVRSERY
                     ILYKTIFNELGFSRQPSKSEKELMDTWFDKFNMDIDLIINACSKSKNISNPSISYING
                     IIKNWNEKNIKNLNDLKQKEEERIVKENINKKQINTTQNNNTYKKTKFHNFNETFTQY
                     TSDELDEIIKKSQKEKFK"
     gene            38048..39511
                     /locus_tag="PC471_000239"
     CDS             38048..39511
                     /locus_tag="PC471_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HDF2787383.1"
                     /translation="MVIKFNQKVRRTLSVAILFMVLIIGCFIEKDDSLETYKEKTNSK
                     INQYNMDVIFDDETKRLMCNQNVDYINNTKSNIDKIYFHIYPNAFSKKDFAPFEKSEM
                     TRAYPNGFNEGYIDIKNILNNNSKMEYKIKGDKNDILEIELGKELKPNERMSLDIKYN
                     VKIPNSIGRFGYGENTINVTNWFPIACVNDERGWNLKSYETIGDPFYSETSDFHVKLL
                     IPNKYKIAHTGKLIDDKHDNKKMLYEIEAENVRDFAFILSSKFDVNKVDSKGVAINTY
                     NLNKKLSKKATEVAKDSINIFSELFGKYPYKEFSVVASDFFIGGMEYPMLVMIDQSLY
                     NEKNEFLLEYVIAHETAHQWWYSAVGNDEISEPWLDEALTEYSTIVYFEQKYGKDMAN
                     KLIKTMEIQTKSYLSENIFKPANEYKNSTEYSLNVYTKGAIAFNEIRKEVGDKVFFET
                     LNEYYNKYKHKNANGRAFVELWNSKGVDINKIISECK"
     gene            39613..40410
                     /locus_tag="PC471_000240"
     CDS             39613..40410
                     /locus_tag="PC471_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDF2787384.1"
                     /translation="MLKYCSVGSGSSGNCHYIGYKNTNILVDAGLSGKRITTGLKDIG
                     VDADKLKGIFITHEHVDHIKGAGILSRKFDIPVFANIKTWCSMKDKLGDIKDKNMKVF
                     ENDKTYSLGDIIVRPFSIEHDASDPVGYNFYTESDEKMSIATDIGCITQNIKKHLYKS
                     KLVVLESNYDPNMLMMGSYTYALKKRVMSSTGHLSNEDAANFCVELINEGTESILLAH
                     LSKENNFPELAYETSKGVLASNDIVVGQDVKLDVLSRNDVSNVYEMK"
     gene            40423..42525
                     /locus_tag="PC471_000241"
     CDS             40423..42525
                     /locus_tag="PC471_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC/EAL
                     domain-containing protein"
                     /protein_id="HDF2787385.1"
                     /translation="MLKRNFENVEDNKILYSIKQGMILAIPAIMTGSTALVILNLPIK
                     AYQEYLSGLFNGEVTNILNFINDSTLGIISLIILLTISYSYGKIYGSKYTVLVPIVAM
                     CSFLVFSHGNELNSYIEIFKTKWLFTSIIVSMTSSVLFVKLTESFVTSVKFHTEGADA
                     DFNMVVSAIVPFIIVVFLFSIFRVVMISLIGSSNFQDIFYNLFSNVFNKMGTNLMSAL
                     LFIFLMHFMWFFGIHGSNVLDTVAKNLFENSMAININLVNNNQLPTEIFTKTFFDTMV
                     LLGGCGSLLCLVIAIFLSEKRINVRKLAKIASIPALFNINEMLVFGIPIVFNYIMFVP
                     FVITPIILTVTSYVAMSTGIVPCTVSAVEWTSPIFLSGYMSTGSIRGSLLQVFNLCVG
                     VMIYIPFIKMSQNRYTYMFKNNIENLTNLLKRYELTGEQPNLLNHNGKIGSISKMLAS
                     DLKFAMKRGEIELFYQPQVNYNGNVVGAEGLLRWKHSIGGFIYPPLVIILAKEEGFLD
                     ELGEYIINKACIDLKKSEKLLRNPVKFSVNISPDQLDDPKLPEQIKNIISSNDINPNM
                     LGIEITEQIALSGSQIIIERINAIHNLGIKLIMDDFGMGHSSLIYLQNNNFDIVKLDG
                     SLVKEILTNKRSSEIIMTIVNLSKNLDFDIVVEYVENIDQRDKLYGLGCEIYQGYYYS
                     KAIPFEEFVEYANQEREI"
     gene            42593..43072
                     /gene="rlmH"
                     /locus_tag="PC471_000242"
     CDS             42593..43072
                     /gene="rlmH"
                     /locus_tag="PC471_000242"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001027003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HDF2787386.1"
                     /translation="MNISIVVVGKIKEKYLKLGIDEFKKRLSKYCKLETIELDDEKAP
                     ENLSVKEMEIIKDKEGKKILGKIKHNSYVIALAIDGKGLSSEELAKTMSDLAVRGNSS
                     LCFIIGGSLGLSDEVLGRADYRLSFSKMTFPHQMMRLILLEQIYRAYRINNGEPYHK"
     gene            43221..43532
                     /locus_tag="PC471_000243"
     CDS             43221..43532
                     /locus_tag="PC471_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDF2787387.1"
                     /translation="MIKIMLACSAGMSTSLLVTKMESAAKENGIESQIWAIPESTIQN
                     EIEKCDVLLLGPQVRYVLPKAQEIAKPYNIPVEVINMMHYGTVNGEAVLNRAIELNNT
                     K"
     gene            43580..43897
                     /locus_tag="PC471_000244"
     CDS             43580..43897
                     /locus_tag="PC471_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HDF2787388.1"
                     /translation="MDEKTIEISFNIIGYAGEGKGLAFEAIKEAKNGNIEKAKELLKE
                     SREVINKAHRYQTELIQNEASGNRTEISVILIHAQDHLMNGMNFQQLAEEIVDLHLKL
                     QDK"
     gene            44110..44811
                     /locus_tag="PC471_000245"
     CDS             44110..44811
                     /locus_tag="PC471_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDF2787389.1"
                     /translation="MLRNQPLYIQVVDILKQKILNNEYKIDENIPTERELEQEFGVSK
                     ITIRKAIEILESEGYVEKKSGKGTKVISNSLFNKLSKAESFSSILLSKGHKLSKETIS
                     IEKIINDTNSQVFSIFGEKCSKITRMYYLDSKPYIFFTHYIPFDENLTVESFNNESSL
                     YMYLYRRNINITRFEDEFFIEESELEVSKALNLAKDILLGRKRKTYDENDNIVELSFA
                     RYNTEVQNYVIKYEI"
     gene            45035..46336
                     /locus_tag="PC471_000246"
     CDS             45035..46336
                     /locus_tag="PC471_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDF2787390.1"
                     /translation="MEKFASFLERKIMPVAAKISNQRHMRAVRAGIIATLPLTIVGSF
                     FTILLNIPIDAYMDFIAPFKAILDIPFRYTVGFLSLYATFGIASSLAKSYDLDTTGVG
                     MLAVMAFLVSTIVPTQVVEPVGGVIEAGRWMDMAKLNSPSLFGAIVTSLITVEIYRFM
                     KEKNITIKMPAGVPVEVSNSFTALLPTAVILMLFWVIRHILGFDISVALSAMLSPLKG
                     FLSGNSLGGGLLTVFLITLFWVLGIHGPAIMGPVIRPIWDMSIAENITAFTEGVSAHA
                     MPNIFTEQFLQWYVWIGGAGATLALVVMFMFSKSQYLKSLGRLGFLPGLFNINEPIIF
                     GAPIVMNPILGIPFIIGPIIMTILSYVLTITGVIPMMVARLPFAMPAPIAAVMSTNWS
                     ILAGILVLINFVISFAVYYPFFKVFEKQQLQREQEEMSENL"
     gene            46473..47567
                     /locus_tag="PC471_000247"
     CDS             46473..47567
                     /locus_tag="PC471_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HDF2787391.1"
                     /translation="MERRLGISVYPEHSNMEDDKAYIKMASKYGFKRIFMCMLSITRP
                     KEEVKKHFKEIITYARELGFEVILDIAPNIFDILQISYDDLTFFSELNASGIRLDYGY
                     DGSKEAMISFNPYDLKVELNMSNDVAYLDNIITYKPNVNNIYGCHNFYPQRGTGLPYD
                     FFVRCSNRFKKYGIRTAAFVNSKYATIGPWNINDGLCTLEMHRNLDIATATKHLFATG
                     LIDDVIVGNAYASEDELKRMSEVNKYQVVFNVVTEEKTSEVEKTIMANEQHFRRGDIT
                     NQVARSTEVRKKYAKENFPKHDNTTIFKRGDVVIGNDDFGKYKGELQLILQDCADERK
                     NKVGRIKEDEIILLDFIEPWSKFKLILEGE"
     gene            47569..48702
                     /locus_tag="PC471_000248"
     CDS             47569..48702
                     /locus_tag="PC471_000248"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase/deacetylase family
                     metallohydrolase"
                     /protein_id="HDF2787392.1"
                     /translation="MKIDILIKCGKTVDKSIIDIAILDDKIIEVKNQIDDSKYQANQI
                     INLDGEKFISAGWIDIHTHCYERLDLYKDYPDEVGIKSGVTTVVDAGTTGALDIGKFY
                     EDTKKYKTNVYALINIAKQGITSQDELSSMMNIDEYELRRAVKKYKDFVVGIKARMSK
                     SVVISNDVEPLKVAKRIKNELNLPMMVHFGSSPPTIEDIFDYMEKGDILTHIYNGKPN
                     GILRGNEVKKEIIEARERGIILDVGHGTESFSMDIAMKSKDAGIFPDTISTDIYIKNR
                     INGPVYNLSTTMEKFIYMGYSLEDIIDKVTKNAADAISLKNKGLIKEGYDADLTIFDV
                     VNEEKELQDSLNKSVITSTSIKPRAVVVNGEYLNIGKSIGENE"
     gene            48720..49829
                     /locus_tag="PC471_000249"
     CDS             48720..49829
                     /locus_tag="PC471_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DgaE family pyridoxal phosphate-dependent
                     ammonia lyase"
                     /protein_id="HDF2787393.1"
                     /translation="MSSIFEKYSVRRVINASGKMTILGVSTVDKEVIEAIGEGCKNFF
                     IIEELINKTGEYIANLLGCESALIVSSASAGIAQSVGAMIAKDDMSLVYNVNNPKKQV
                     KREIIIPKGHNVDYGVPVEVMIRLGGGEVVEAGYANMCNKEHIIREINENTAAIMYIK
                     SHHCVQKSMLSVLDAVQVAKEYNLPLIVDAAAEEDLNVYYELGADLVIYSGAKAIEGP
                     SSGLVIGKKKYIDNVKLQSKGIGRAMKIGKENIVGLTCAIERYISNDKVTLKEMEDKL
                     NPFIDKINTIKGVSASITRDSAGREILRGEIDFNEDIINKSTQQIINELRSGEIAIYT
                     RDYKANEGKIEIDIRSVTGSDLDTIYNRIKNIVES"
     gene            49894..50634
                     /locus_tag="PC471_000250"
     CDS             49894..50634
                     /locus_tag="PC471_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KDGP aldolase family protein"
                     /protein_id="HDF2787394.1"
                     /translation="MNCIPQYYKNRVCLNVLAGSIKNAKEVYDACDGHVLIGILSKNY
                     STVEAAIEDMKKYSKEIDNAISVGLGAGDPNQSNMVSQISKELQPQHINQVFTGVATS
                     RALLGQNDSIINGLVSPTGKPGFVKISTGPLSSKEKDGIVPIETAIAMMKDMGGSSIK
                     FFPMGGLKTKDEYIEVAKACAKHNFYLEPTGGIDLDNFKEIVQIALDAGVEKVIPHVY
                     TSIIDKETGETKVEDIGVLYKIMKELLG"
     gene            50639..51631
                     /locus_tag="PC471_000251"
     CDS             50639..51631
                     /locus_tag="PC471_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HDF2787395.1"
                     /translation="MNILAFGEIMMRLSVPDYKFLTQTNELNYITTGTGLNILSGLKN
                     FGYNTYMLTKLPNNNVGKASSANIRKLGVKDDFITYGGNHIGVYFLENGYGERPSEVT
                     YLNRLNSSFCESKLQDYDIDKCLEEMDIVHICGIAMQLTDSVKEIALELARKSYEKGI
                     KVFFDFNFRQSLNPERTYDDLVNDYKKILPYCHGLFASYRDINKILNIDSLNIKSSSE
                     LIKKEYDIDIVAGTMRRFDDMNNRFIKGYISNDDGYFESREYKLEIYDRVGAGDGYVA
                     GIIYSYLENMKSEDIVEFGVASSVLAHTTYGDNSLVSTGHVLRLMNNEKIDILR"
     gene            complement(51819..52700)
                     /locus_tag="PC471_000252"
     CDS             complement(51819..52700)
                     /locus_tag="PC471_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDF2787396.1"
                     /translation="MNTKQLEYFISVAENLSFTKTAEKFYISQTAVTQQIKALEEQIN
                     VTLFTRSKRHVELTPAGKVFLSEARTIIKNINDAIAKTQQFAHGFFGTLSIGTLIGYE
                     KNKLQQYLKEFSNTYPNISMDICTNEITELLNLVKNNSMDLAFVINPENQPLKDFEYK
                     TVERYSLVALLPPGHPLYNEDSIDLIELQHDKFIFVKETGDEYGQKSMIQNRYREAGF
                     VPNVVQRSNNLNTIESLVSSNMGISILPSFCVTDTMIERDIAIVPIKEVENRIEVVVV
                     WNKKNTNPALEKFISII"
     gene            52973..54361
                     /locus_tag="PC471_000253"
     CDS             52973..54361
                     /locus_tag="PC471_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2787397.1"
                     /translation="MEDKFYAKGNGNNGYIKNLEVCSFNNLDGTCGMFQMALYKRDEK
                     YYLYGCCFGGNKKNGVMISDITDPYNPQFIKHFQMLDPKEYPTTTTPKIQIADDLMIV
                     AMSCGSGPGALVDQAKLANIKCEAGIRIYSLKEDPLNPKFLGYWDCGLKHVMGVHRFM
                     YNGGRYVHLSSDCVGFEGLIYRVIDIINPTNPVEIGKWWRPDQYANGYPNRTFDAGAP
                     HCPEFMDKGWLHGPPFVRDGKAYCGYGGAGLVVLDVEDLTRPRCLGELPFMPAFSSRL
                     AGARTHTALPLPGRDLVVVQNEGERFQFFKPDNITDVQAMNNIHMVDVSDPTKPTLIA
                     QFPYPEVPKDFPYPNFNVAGLGKPGPFGPHNLHEPMDNKPWLEQRGDRVYCCYFHAGL
                     RVYDVSDPYYIKELAYFIPPNPNKTPEESYFPGFPGPRLAVTEDLIVDDRGYIIIDAL
                     DDGFYILKMKED"
     gene            54389..55342
                     /locus_tag="PC471_000254"
     CDS             54389..55342
                     /locus_tag="PC471_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HDF2787398.1"
                     /translation="MKILGISFGTKNGNNDTMCKVALKGAKEAGAEVEFIQMSSINIK
                     HCTGCCACVKTLLSGKGSMCVLKDDFEWLLDKMKDADGIVVSDPIFEEGASGLFHTIM
                     DRFGPRADRGNNIIGTKVAEAIGGKIPDSRMLNDKVISFMGIGGSDWGTRVQCEHAML
                     ALIPMWKVIDNAWFPWAKELVMDDTRLVQVHQIGLNIVEAAKDFEKASYQGEEGVCPH
                     CHNRLFYLEPGTKKAICALCGIVGELDTISGKTIFSFPEEQLGHAHDTLPGKFIHGDD
                     IKKMEGHYAEVRKTQEFKERKTSYNFIEPLTKEKQAGVSLG"
     gene            55402..55626
                     /locus_tag="PC471_000255"
     CDS             55402..55626
                     /locus_tag="PC471_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2787399.1"
                     /translation="MNEKSSFKQIEIILAVIFLVLTYYKVRLTPAFIVIFIPLYLSYF
                     AVSMIVNQIAIKLLEIRQAREATNYNIGNG"
     gene            55619..56131
                     /locus_tag="PC471_000256"
     CDS             55619..56131
                     /locus_tag="PC471_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HDF2787400.1"
                     /translation="MDRKKKEILTLSMGIALLPPLWAVLAPYIGIKTGAVALICAGLY
                     VTNGNKQKDGLKIMFGFWCGDLWAVLAILIMGYMNFNQNLELFLTLSILGFFAVVIAS
                     LFEKIIFLPSWLCGWAIGLTIMTGENIINLQDICIQIAVAMAVGVWYVGAGVDLFTIY
                     ILSKDRKKGN"
     gene            56163..57017
                     /locus_tag="PC471_000257"
     CDS             56163..57017
                     /locus_tag="PC471_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase"
                     /protein_id="HDF2787401.1"
                     /translation="MAMGFEFKIMRSLIYVGLAKEEYRPKLMDWLYRHHIPDSISTFG
                     PYCTKYAFYQAYPTPNEGERFGARKMQLTEHYWLVDEHMPEMANRIMTEYMPMDVLRW
                     QGCIPDVENKRVHENAESGDAGRAVGGDNGCPPFIFAFVPINWEEDFRGKGRTVQDGP
                     NYRWQFMIKYPDGISKEEGEKWFYDEVVPYFTNCCYVNRFVSSKIMINYGATAFDRVS
                     ELWFEGEEEWYKAVVEETKSFIKKPEWAQEEEFPYLKPQFNIASVFLGDIATMDAYSQ
                     YRGYIPMR"
     gene            57551..58345
                     /locus_tag="PC471_000258"
     CDS             57551..58345
                     /locus_tag="PC471_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDF2787402.1"
                     /translation="MYTIGQVSKFLGISRDTLKFYEDKGLVNPKKNDENGYRIYNQVD
                     IYDIATINFYREIDIEIKKIQEIRKSKSINNLELLLEEKEQIILKEIEYKKLLLKKVQ
                     LVKEDCDKIKTYFGKYTIKEMKPLEVKGKIKHVTNYEEYEALKENTNNLKKAVTLTDL
                     RRVINFNEEGIIEEKFIIVREVDDYNKIIEGEVLSHPNCIYTIIEDGRWSTGGENIDN
                     KVEDRLREIAIENECELLGIVYVNILLTTYEEGIERIFLEIYAPIK"
     gene            58449..59255
                     /locus_tag="PC471_000259"
     CDS             58449..59255
                     /locus_tag="PC471_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HDF2787403.1"
                     /translation="MRTEKSLKRPIASFIILTNIIFLPLFLLVVVTIMLKLPTVISDI
                     ALCISAWSSTFAFMILFKKIYPGKKFLVHVKDRFRNKLKFSTVSIIISIQVLIAVVVI
                     FILASKNHGIMPNFTVSSWGAFIYLFLKNLFAGSLGEELGWRGFVQNHLQKRHSPLKA
                     SIIVGFWWGMWHLPIWFTTGFKGLDLIKYIIFFMIAIISISIIMTTFYNLNKTLIIPI
                     VIHQLFNFLIGIINGDLIELINYYAMLYLIFAIALIVINPGNVLYKNIRE"
     gene            59317..59838
                     /locus_tag="PC471_000260"
     CDS             59317..59838
                     /locus_tag="PC471_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003374619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HDF2787404.1"
                     /translation="MVLLIVDAQKLITNERLYKFNEFVVNVENLIDTARKNNIEVIYV
                     RHDDGVENELTKGKNGFEIYEKFKPCNGEKIFDKKVNSAFKETGLLEYLTNNGEKDII
                     IAGLQTDYCIDATIKCGFEHGFNIIVPAYSNTTVDNFFMSAEQSYKYYNEFIWNERYA
                     KCISLEETLRKMK"
     gene            60253..62340
                     /locus_tag="PC471_000261"
     CDS             60253..62340
                     /locus_tag="PC471_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HDF2787405.1"
                     /translation="MVVDKEIVSILQILLNSTLITTNGLQEESNSSKRQITYRINKIN
                     DMLKVKNVPLICLRADKDIIVRKETKKAIKEILEKNYSKNTYYFSKTERLLYMYLMLF
                     INMDDISINHFINSIKVSRSTVNLDFKDLIPELEKKNIKVKNNRINGYYIVGNEMEIR
                     RVLIKNIIETLSNEKSSRVFDIFIKEYNLDSFEEAKKIILKLIKKYEITFVEDRLVEF
                     IYIFIFLKARMYSTRIVTQENVDIPNISVMSSMKEYKFAKELLEVYGSEDKIKPHNIM
                     YISAWILGISVGNYEEDTEDKDVISKIVNKMMTKFGYLSGVYYISQEKIFKQLYSHFR
                     PAYYRLLFKLPIYNPICEKVKEEYKLVYRIVSNAMKEFCGLFGEEIQEEELAYLTMHF
                     ATLFSNKKEFDGFRKRIALIVCSNGVGSSAILHAELTNLFPDLHFIPMSESVGLENIS
                     EPVDIVFTTNYNAEGLKTDVPVIKVSPVMTTKEKYKITREVYIQLGDIFLRQPKIDEV
                     MDIIKKYANVTSESILSSELLAYFTQIENFTSKEGEGPMLSEITNETLVKIKVKAKNW
                     EEAIRQSASVLVENNKVTEGYVDAMINSAKASGPYIVITKHVALPHARPEAGSKEIAI
                     GIATLDSPIEFGNNENDPVKYVFCLSAIDNNSHLRAMSELVELLEEDEFFKVLDSAKE
                     AKEIIDFIKTHEL"
     gene            62355..62642
                     /locus_tag="PC471_000262"
     CDS             62355..62642
                     /locus_tag="PC471_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDF2787406.1"
                     /translation="MYKILIACRAGVGSSLMLKIKTQQIIKENNFPIEVEHGSLDSLN
                     GFNGDAVETLIDVAEELKNKNLKFDIIGIHNIVDRNEIKTALEKFLASKEG"
     gene            62669..63940
                     /locus_tag="PC471_000263"
     CDS             62669..63940
                     /locus_tag="PC471_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HDF2787407.1"
                     /translation="MEFIISLLSTPAVLLGVVACIGLILQKKSAVEVFTGSSKTLIGF
                     LIFGIGASAMTGALQNFNKLFQHGFGITGVIASPEAATALAQGTYGFAVSCTLILGFI
                     LNLVFARITKFKNIFFTTGHSLFFSCVLVLIMKAHGFDNTITILIGGTILGFMSAALP
                     QFCQPFMRELTGGDEQAIGHFNMIGYGLSGYIGRLFSKHKDDTTETIEFPKWLSIFRD
                     FLMGLSIVMLILFYIATLKAGQAFTQEIAGSTHWLVFPLIEAFTFVAGMSILMSGVRM
                     FLAEITAAFVIISEKYIPGSRPALDVPTVFPYAPNAVIIGFISAYAAGLLAIAIMASF
                     PSIFPVVIIPAAHICFFSGGTAAIFGNTSGGWRGAIAGSFVVGLLLAFLPVILYPVYG
                     TLGIEGATFPNIDYNVVGSILHNILQFIKPI"
     gene            63998..64597
                     /locus_tag="PC471_000264"
     CDS             63998..64597
                     /locus_tag="PC471_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HDF2787408.1"
                     /translation="MENTLSEFLDNISNELSEFIASIDETSINKACELILEAEKNHGR
                     VHVTGIGKPGHVSGYISSLLSSTGTSAYILHGTEAVHGSSGQVVEGDVVIAISNSGET
                     QELKATLKTLKVNGAKIIGVSGNESSFLKNISDIFLFAGVKQEGDCLNKAPRASILAE
                     TIILQSLSVVLQYAKGLNTQEYLKWHPAGSLGKSIREGI"
     gene            64602..65507
                     /gene="dapA"
                     /locus_tag="PC471_000265"
     CDS             64602..65507
                     /gene="dapA"
                     /locus_tag="PC471_000265"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HDF2787409.1"
                     /translation="MKYKGIICAMITPFDENQNINSQATCQLIDYLIEKGIYGLFILG
                     TNGECHVLTDDEKVEFAKIVINHTNNRVPVFVGTGGNSTREVVDLSKRMEAIGASALS
                     IITPYFVTPTQQELILHYKTIANSVNLPIIMYNMPGKTGINIEPESVRELSKIKNIVG
                     IKDSSGKLDNMKAYIEATKNENFSVFSGSDSLILDSLKAGSQGAVAATTNFLTEIGIS
                     IYENFMKGDLEEAQKSQSSIEELRRILKLGTIPSVMKKTIVLNGINVGTARLPVTEPT
                     GEVLEEIKRVVENYRTILNKSSENR"
     gene            65613..66245
                     /locus_tag="PC471_000266"
     CDS             65613..66245
                     /locus_tag="PC471_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketohydroxyglutarate aldolase"
                     /protein_id="HDF2787410.1"
                     /translation="MKKMNITKRMSECGALAIVREENLNRACEIAEGCIKGGITVIEM
                     SYTLNNAGEIIQGLNKKYGEILCVGAGTVLDSETARHAILHGAQFIIAPNYNEGVAKI
                     CNRYQIPYAPGCTSLTEAVDALSLGATFIKAFPISDFYGPKLVKVFKTPIPDMPVLAS
                     GGLNIENLHIWLENGVDVCGFGGLLTKGSVEDIAKNANKIREIIKNVRKI"
     gene            66722..69229
                     /locus_tag="PC471_000267"
     CDS             66722..69229
                     /locus_tag="PC471_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HDF2787411.1"
                     /translation="MKEDIRLHRMCKKEQATKNEVISEDILKSMSIGVSKHFLDDSFT
                     IIWGNTSFYQLLGYTEEEFLVKFSSLKDYYEQNLYHFESMKNHFLEAYHHGEKSIEYQ
                     ICISANNGDPVWVIMTGILIESQDNEKSMVYILYSKINELKIKQEELSDMEKESTENF
                     KWMMSEYAGNVYISDMDTYELLYLNKHSCNTLQASANELIGRKCYEVIQGRTSPCPFC
                     TNSYLKKDDIYEWEFYNKKLKRTFIIKDRMLNWQGRRVRIELSYDMYSAEYKLAKKDQ
                     ERESILKTIPAGMVRLDARDYLTILWCNDIFLNMIEYTKEQFAEELNNQCGYLFSEDY
                     KRSKKLAQDLKESGDNVVFEAKIYTRSREERIWTVTLCYISGEDSWDGIPSFYSIGID
                     ITKERKQIEKLQHISEKDALTGIYNRAETERQIKKYFEKNLNVMGALFMIDTDNFKQI
                     NDTEGHMIGDIVLTEMASGMKKIMRDSDVVGRIGGDEFTIFMKNISSVKDAEKKAEEL
                     LYMFRHLFQKEKSFLKVTCSIGIAIYPKDGTTFKEIYARADKALYQAKNMGKNNYVIY
                     NQDYFKELEEFDCSSLGTVIDSEKQYAEYPDNLTRYIFRMLYQTDDIDQAINMILEVV
                     GKQFDVSRVYIFENTEDGRYTSNTYEWCNEGISSQMAYLQNSNYQDYEDYEKLFEDEL
                     VFYCRDIHTLSPKLEELFSSQGIHSTLQCAYKEDRVFSGFVGFDECTGLRLWSQEEIS
                     TLSLISQIISMFLQRKKNNKLNQQIQQYQNILDNIDQCICVINKENNFLLYVNKKFKN
                     TYSKFQIGQPYYLDSIDIHKISIIWNKKEAYLCTSTI"
     gene            69571..71733
                     /locus_tag="PC471_000268"
     CDS             69571..71733
                     /locus_tag="PC471_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HDF2787412.1"
                     /translation="MKKIIYVLFTIELLIVSLLGLNIVKDNEINNILYNDTTSISVMF
                     KDYKKLNKNYSKWIKDIADENNVAISKYVFNNNEKLSIYTTDTSLNNNIKLKSGKFPN
                     TNSNEFISNINTNNSNQVGKFSTMSKDISILIRDFGKISEVGESGILYISTQNEDTIN
                     KILRELNVDRSIFVARLHDRYVNSNLYLNPSIIRDLILIILCFLATIIHYSISVSREA
                     SILRLNGYSKLDIIARVIKNILRIMILSSVTALLIYIIYILKLNIGVRACLYFAMFSI
                     ICILINILISILIVGFNSRSSKYVLSLNGKKSYGFVNIMHFTLKIVFVSFLILSINNC
                     ILNYNMLQTQLGNLSEWDKAKNVYNLTLKDTGEQSLGKEEVIKNAKIKGFYKNLVEEK
                     DAFLIDSTNFEKLEDGSYMYEINSKGKNPEVSQYGKNIKINKNYLKVNPIYSNGKEIF
                     NLIDYDDKTINLLVPKKLQVHENEIKKEFRELFYFEKIEVENMYNKELNRELNKTKKS
                     DLNVNIIYVDNNQSYFTYNSLVMDDNRNLIDDPIAIVDIDNIDDSFYLSYISRCVYFN
                     SKKLDALADISSIIEAQGVEAHIQSLHAVYNEYGLEINKLEKYLNSEIFTIIIIAISN
                     LMITYNIVASYYERNKYKLYIKKIFGYSTTARSMLLIVSLILTNIIPIGIISTRVDLS
                     NNIILFGLLILVIEVIVSIVLDKIISNSSFNKIIKGEH"
     gene            71738..72355
                     /locus_tag="PC471_000269"
     CDS             71738..72355
                     /locus_tag="PC471_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2787413.1"
                     /translation="MISLINISKKFKDKSIIDKFNLEISKGEFVAITGNSGSGKTTLL
                     NIMGLLEKPNSGDIIINNIKNPNSKQIRILQRDFYGYLFQNYALIENENVENNLKIAL
                     KYQKNINHNEKINYALESVGLRKYNKKKIYELSGGEQQRVALARVILKECEVIFADEP
                     TGNLDKKNRDIVFDILKSLNKNGKTIVFVTHDLDLSEQADRVIKL"
     gene            72544..72888
                     /locus_tag="PC471_000270"
     CDS             72544..72888
                     /locus_tag="PC471_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000212737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HDF2787414.1"
                     /translation="MENLPNCPKCNSEYTYEDGTLLVCPECSHEWALGLGAEEDSNII
                     KDVNGNVLNDGDSVTVIRDLKVKGSSSSIKIGTKVKNIRLIHDSSDGHDIECKIDGFG
                     AMKLKSSVVKKA"
     gene            73458..74324
                     /locus_tag="PC471_000271"
     CDS             73458..74324
                     /locus_tag="PC471_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HDF2787415.1"
                     /translation="MIDIHKNIYDNKLFEELKIDCKKCFGLCCVALYFSASDGFPINK
                     ESGKPCINLQPDFKCSVHNSLIKRGFKGCTAYDCFGSGQKVAQVTYKGIDWMQSPELT
                     NQMSEVFLIMRQLHEMLWYLKEASVLNISDTIKSKIDLIIEETEKITNMGPEQIINLD
                     IISHRTKVNLLLSQASESMMGKVKSFIKTSTLKNMKKLSKNIDLIGADLRKINLIGAD
                     LRGRFLIAANLRNTDLSGANLIGADLRDCDIRGANLENSIFLTQLQVNTAKGDSSTKL
                     PASLIRPKYWEK"
     gene            74437..74841
                     /locus_tag="PC471_000272"
     CDS             74437..74841
                     /locus_tag="PC471_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmaF family protein"
                     /protein_id="HDF2787416.1"
                     /translation="MSYQNYKCERDYVYDDYKYNKCNKCYNDYEEMTHVHEYSESVKL
                     AEECEDRHNHRAAGVTGEVIPINGGTNHVHKINDNVDFLDHFHKICVTTGPAIRIPGT
                     DKHIHLICGETTVNDGHCHKFLFTTQIEAPLV"
     gene            75005..75454
                     /locus_tag="PC471_000273"
     CDS             75005..75454
                     /locus_tag="PC471_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDF2787417.1"
                     /translation="MKSGIILEMKQINSTEYDEMLQVWESSVRATHDFLTEKDIESLI
                     PLVEIGLKEVENIVCIKDNDIIKGFIGIDKDKIEMLFIEDKYRGNGIGKKLIKYAIDK
                     YNVKYVDVNEQNKKAVGFYIHLGFKVFDRSEIDGQGNPFPILHMKLI"
     gene            complement(75610..75987)
                     /locus_tag="PC471_000274"
     CDS             complement(75610..75987)
                     /locus_tag="PC471_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDF2787418.1"
                     /translation="MLTPYLIFNGTCEKAFNFYAEAFGGGKTIFARLDSNPNNPIMHA
                     SVTFTKYEGCIMGADTDKPVVISGMAICVVLPSREAIEEISVKLAEGGTLVQEFLPHP
                     PPHQNDGAAEVLDRYGYTWYLST"
     gene            76249..77166
                     /locus_tag="PC471_000275"
     CDS             76249..77166
                     /locus_tag="PC471_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2268 domain-containing protein"
                     /protein_id="HDF2787419.1"
                     /translation="MVNMNIIEIRSDKIYKKIMDAPINKKEDIYRYELMKPFEFKWKC
                     MNVPIVARQKGGYDVIIASEMLGVLSPKDIDEKQKKNINVLSADKIWGTCKETIENSI
                     NAFIKEGYDLNIKDYKYSILLANPNSPYTILSDGYWGDGGIPGYIFLSLVPNEYTINR
                     LPVLIAHECNHNIRFQFIEWNNNITLEEMMINEGLAENFATWMFGEEMLGPWVSRTDI
                     ETLNTYIKPIIKSALKETGFQNITSYLYGDDIAKMQGYFPVGLPYCAGYACGYYMIKY
                     YLEKTNKSIIEATLLPYSEIIEAVKEFWE"
BASE COUNT        29580 a         9143 c        12876 g        25688 t
ORIGIN      
        1 atataaatta acctactgtt taattttcaa tgttcgtgtt tatttcttgt tccttttttc
       61 aaggacaagt aatactatac catctttaaa acagtaggtc aatacttttt ttatattttt
      121 ttataaattt ttttcatatt cttttttcag cttctctact acctttttct ctagtctaga
      181 aacagtcatc tgtgatatcc ctagttcttt agctatacta gattgtgttt tatcaaagaa
      241 aaatctatct ttaaaaattt taacttctaa ttcatttaaa gtttcaataa acttattaat
      301 aaaatcttca tactctatac ttccaaagtt accttcttct tttccaatct tatcaataag
      361 agttatatct ttatcctctg aatcatcatt acttgatgaa tccaaagaca ttggttgata
      421 tccataagaa gcctccatag cctctaaaac atcttcttca ctaacgccta tataatcagc
      481 tatatctttt acctttggat gcttcttatt ttcttgttct aatttaatct tcgcatcact
      541 tatctttttg cttaattcct gtattcttct tgggactcta agtgtccata ccttatctct
      601 aaaatacttt tttatttctc ctactatagt tggtgttgca aagctggaga actcaaatcc
      661 tttctcaaca tcatatctat caatagcata aattaatgcc aatgaagcaa cttgatatat
      721 atcttcaaaa tcaacacctt tgtttatata cttcttagct aacaatcttg ctaaatacag
      781 atgtctttca ataagaatat ttctaatgtc tacattttta tttttattat ataaattaaa
      841 tagctctttt gtatccatat ttaggtaatg tgtagcatta gctacatttt tcattaaata
      901 tcaactccta aatatttagt catttttatt tttgtccctt gattatgttc tgattcaata
      961 tctacatcat ccattaaagt tttaattata aacaatccaa gaccactctc ttttggattc
     1021 tctaaatctg gtgtacttat tgagctaaca tcatatcctt ttccattatc ttgaatttct
     1081 atattaattg cattatctaa aatattaaat actatattaa acacatcatc tttactgtgt
     1141 tttattgcat ttgtacatgc ttcagatact gctaccttta tatcttctat atcatctatt
     1201 ggaaaaccca tcttattggc aattccagat gctgttaatc ttattatact aacataatct
     1261 ggatttgtag taatttccat ctttatagtc tcacaagcca aagtttatcc ctccacttta
     1321 aatattttat ctagacctgt tatagtaaaa atctttctta cgttactctt aggatttact
     1381 atgtatatct ctttttcatt tatttttaat ttttttaata cacctatcat agctcctaag
     1441 cctgttgagt ctatataatc taaatcttta agattaatct ttacatctaa catatttttt
     1501 tctattaaag catgtaaatg ttcctttagt ttatctgctg tagaaacatc aagttctcca
     1561 tcaagacaaa cattccaaaa tttattttgt gaatctaaat ttgaatcaat attcatagac
     1621 atgtaaaaac ctcctatttc cttttaataa ttttaaatat atttcctata ctcaatattt
     1681 tattaattac ataaactata tcatctattc cctttgatat tgatgatgca ttttcaacaa
     1741 tatcttgtat atgtctttca ttataatcta caatcttata tgccttttca acaaccttat
     1801 ttatgctgtt tagagtttta gctaaataaa ttgctactat aagagcagag cttcctacta
     1861 acactgctcc aatttgccac ccccatgcat tcattagtta acatctccct catcatcaaa
     1921 gcttttactt attactatat cttcttctga aatctttata tcttcttcat tattatcatc
     1981 atccactaaa tttattattc tctctttaac atcattaaat gtttcatgta gaacttcttt
     2041 tggattttct ttgacatcat taaacttctc ttttgtttct tttcttagtt cagaaccttt
     2101 tttaggtgca aaaaacatac caaatataaa ccctaaaata gctcctaata ataaaaatcc
     2161 acctttttta caacttcttt tattgcacat aacaattact cctttcaatt ttaaattttt
     2221 atctaactac attatacaaa aagaatagga tatttatcct attcttcttc aatatttatt
     2281 ttggcaatcg aaactacatt gacttcatca ttcgtcttca ttaatttaac accacttgta
     2341 actcttccaa acagtgatat ttcattgact cttattctaa taagaactcc atcagaattt
     2401 ataatcatta tctcatcatc ttcgttgacc atatctgcac ctacaattgt accagttttt
     2461 tcagatatat tataagtttt aattccttta ccagctctta tttgacttct atactcttct
     2521 atatttgttc tctttccaaa accattctta cttacaacta acacatctgt acctttactg
     2581 caaaggttca tagacacaac aaaatcttct ttacttaaag ttataccttt tacacccatt
     2641 gctgttctac ccatatatct tatatcattt tcatcaaatc ttatagacat accttcttta
     2701 gttactaaaa gtacttcttg ttttccatct gtaagttcta ctccaataag ctcatcatca
     2761 tctcttaaac ctattgcaat aagaccagat ttatttatat ttttgaactc ttctctctta
     2821 gtctttttaa caataccttt tttcgttgca agtaataaat attcattttc atcattacca
     2881 tcaataggta ttagagtagc aattttctca tctgctgaca attgaagtaa attcactata
     2941 gcagtacctt tagcctgtct cttaccttct ggtatttcat atgcatttaa tttaaatact
     3001 ctacctttat ttgtaaagaa taatagtcta ctatgagtag ttgtagttac caagtgcctt
     3061 acaaaatctt cttctctagt tgtaagtgct gaaatacctc ttccacccct tttttgactc
     3121 ttataagtat cagatggaag cctttttata tatccaaagt gagtaagagt tattgctatt
     3181 tcttcatcac ttataagatc tctcatatct atttcgcctt cagcatgtct tatttctgtt
     3241 cttctctcat cagagtaatt ttcttttatt attgtaattt catcctttat tacatttaaa
     3301 agtaatcttt catcagctaa aatctctttt aatctattta tcttcttgat taaatcttca
     3361 tattcagctt ctatcttatc tctttctaaa cctgtaagtc tttgaagtct catatctaat
     3421 atagcttgtg cttggatttc agttaatttg aatttttcta ttaaacctag cttagcttct
     3481 tgcccagtct ttgaagctct tatcaagctt ataacagcat ctatattatc taaagcaatc
     3541 tttaatcctt ctaaaatatg tgctcttgct tcagctttat ttagttcaaa tttagttctt
     3601 cttgtaacaa catcttcttg atgtttaata taatgatata atatttgttt aagatttaaa
     3661 actcttggct gaccatctac aagtgcaagc attattatac taaaagtatc ttccatttga
     3721 gaatgtttat acaaattatt taatactata ttagcattag catccctctt taattctata
     3781 acaattctca taccatttct attactttcg tctcttaggt ctgatatacc ttctattctc
     3841 ttttctttaa ctaactctgc tattctttca accagtttag ccttatttac ttgataaggt
     3901 atttctgtaa ctattatttg ctgttttcct tttggtagct cttctataaa agctctagac
     3961 ctaactttaa cttttcctct tccagttctg tatgcttctg ctatactttc ttttcccatt
     4021 ataattgcag ctgtagggaa atctggtcct tgaacaaatt ttattaaatc atctacacta
     4081 cactctggat tatctatcaa atatacagtt gcatcaatta cttctgctaa attatgtgga
     4141 ggtattgaag ttgccatacc aacagctata ccatttgagc catttactaa taaattagga
     4201 tatctagctg gcaatactga aggctctttt aacgactcat caaagtttgg tttaaagtct
     4261 acagtttcct tttcaatatc tctcagtagt tctaatgata atttactcat tttagcttct
     4321 gtataacgca tagcagctgg tgaatcccca tcaacagaac caaagttacc atgaccatct
     4381 actaaaagtg ctctagttga aaaatcttgt gccattctta ccatagcata ataaacagca
     4441 gtatctccat gaggatggta cttacctaaa acgtccccaa caatacgagc tgacttcctg
     4501 tatggtttat ctggagttaa atttaactca ctcattgaat ataatattct tctatgaact
     4561 ggctttaaac catctctaac atcaggaaga gcacgtccag ctataacact cattgaataa
     4621 tcaatatacg attttttcat ttcttccgct atttcaatag ggagtatttt gttattttct
     4681 tccatgtata ctaatcccct ctctctatat atctaagttt ctaacaaatc ttgcattttc
     4741 ttctataaat tcttttcttg gagcaacctt atcacccata agcattgaaa atacttcatc
     4801 tgcaattgca gcatcttcta cagtaacttg taataaagtt cttgtctcag gattcatagt
     4861 tgtttcccat aattgctctg cattcatctc tccaagacct ttgtaccttt gaagttcaac
     4921 accatttctt ccaatttcat ctagtaagat atttaactct ttatctgagt aaacataatg
     4981 ttccttcttt tgctttgtca ctttatataa aggtggctgt gctgcataaa catacccctc
     5041 atctataaga ggtctcatat atctaaagaa gaatgttaat aataaagttc ttatatgggc
     5101 tccgtctaca tcagcatcgg tcataattat aattttatga tacctagcct tatctatatc
     5161 aaaatcttct ccaataccac aaccataagc tgttatcata ttttttattt catctgaaga
     5221 tagtatccta tctagtcttg atttctcaac attaagtatt ttccctctta atggaagtat
     5281 agcttgacta tttctatctc taccttgttt agctgaacct cccgctgaat ccccttcgac
     5341 taagaatatt tcacttttag atggatcttt ttctgcacaa tctgcaagtt ttccaggtaa
     5401 agatgtactt tctaatacac tttttctcct tgttaattct cttgcttttt ttgcagcttc
     5461 tctagctctt tgtgctctta aagctttatc aactatgatt cttgctgttg atgggttttc
     5521 ttctaggaaa gaccccagtt catcaactgt tacactatca actatacctc tcataaagct
     5581 attacctagc tttgttttag tttgaccttc aaattgaggt tctggtaatt taactgatac
     5641 tactgctgta agaccttctc ttatgtcttc accaagtagg ttaacatcat tttcctttaa
     5701 aaatttattt ctcttagcat aatcatttac agttttagta agagctgctt taaatccact
     5761 taaatgagaa ccaccttcat gtgtatttat attgtttgca aaagaatata tgttctctgt
     5821 ataaccatct gtatattgca tagctagttc aacaaaacaa tcatcaacct ttttatctat
     5881 ataaactatg tcatcatgta taccagttct agatttattt aaatacttaa tatattctac
     5941 caaaccacct gtatagtgaa attcttttct tttttcttgt ccttctcttt tatcttcaaa
     6001 tactattttt attcctttat tcaagaaaga aagttctcta agcctatact ccaatgtttc
     6061 atatttaaac tctatttcat caaatattgt ctcatctggc ataaattgaa tcattgttcc
     6121 tttatcttga gattctccaa ctacttcaag cttagatgtt ggcaatcctt tctcatatgt
     6181 ctgcttatat atctttccgt ttaaataaac ctctgctacc atccattttg acaatgcatt
     6241 aactacagaa acaccaactc catgaagacc tccagatact ttatatcctc cgcctccaaa
     6301 ctttcctcct gcatgaagat tagttaatac tgtttctaaa gttgatttac cagttttagg
     6361 atgtatttca acaggtatac ctcttccgtt atcttttact aaaacactgc catcttcatt
     6421 aatagataca tatatgtctg aacaatatcc ttgtaaagcc tcatctatac tattatctac
     6481 aacttcataa actaagtgat gtaaacctct agggcttgta ctaccaatat acatacctgg
     6541 tctttttcta acggcttcta atccctctaa tacttgtatc tgacttgcac cgtattcttg
     6601 tttcatttaa tttcctccat tctctatgct aataacatcg ccattttcaa tattaaatat
     6661 tgtaacattt ttttcatcaa atattttttt atgtgaaact tcagcacttg ttataaacat
     6721 ttgaacattg cttaaattat ttactaaaag tttttgtctt gcttcatcta actcactaaa
     6781 aacatcatct aaaattaata ctggatattc ttcaacttca ttttttatta attctatctc
     6841 tgataacttt aatgatattg aagcagttct ttgttgacct tgtgaaccaa aaagtcttgc
     6901 atctaaatca tttatgaata tatttaagtc atctttgtgt ataccatatc tggtagtctt
     6961 agattctata tcattaggtc tattcgatga caacttggct aaaaatttat tatatactgt
     7021 atctatagta tcttcatcag ttatatttat ttgattttta tatcgaattg ataatctttc
     7081 aactccatta gtcaaattca tatgcatatt ttcagatata ttagctatct tttttataaa
     7141 gtctctacgc aaaatataaa tataacttcc atactttgct aacgtatcat catatacatc
     7201 taataaagct tcatcaacat gtatattttt aagcacccta ctcctttgag aaagtgtttt
     7261 attataattg gtaagatatt tataatattt aggtataatc tgactaatct ctttgtctat
     7321 aaatgccctt ctttctttag gaccctcttt aaccaacctt aaatcctcag gagaaaatat
     7381 aacaacattt aagttgccaa gtagttcttg tatcttttgt aaaggaactt tatttattct
     7441 tatccctttt ttatcttttc caataattag ctctattaga ctatatttat tatattttga
     7501 aaaacttcct ccaatatata agttttcact attaaatcta accatttctt tatctttgtt
     7561 agtcctaaat gattttccaa aagaaagcat ataaatagat tctactatat ttgtctttcc
     7621 ttgaccattt ttaccaacaa gtagatttac tttcccatta aattccagat gcaacttttt
     7681 ataatttcta aaattaacaa gttgcaaact tttaagtttc acaaaaagtt ctcctttttt
     7741 tacaatactt ttattttagt tccttctatt tcaactatat ctcctgattt tatctttttt
     7801 cctcttctta attctatttc atcatttact gtcactaaac cttcttgaat taaaaatttt
     7861 gcatgacctc ctgttgatgc aatctctgct aatttaagaa attgatccaa ctttatatat
     7921 tctgattcta tagttatttc agtcataata gttcctcctt gtcactattt taaacaaatt
     7981 cctattgata cactaaaagt atattatacc ataaatatgc ttataaaagg agttgtctaa
     8041 gattagaaaa attttctaca aaagacaact ccttcatgtc tgctatatat ttgatgatat
     8101 tcttactgga agcagtaagt aaatataatt aacatcatct gttggtttaa ttatacaagg
     8161 atttacattt gtagtaaatt ctataaatat ttcctcatta tctatatttt tcaaaccttc
     8221 gataaaatat ctagaattga aagctatatc taggtaatct ccatctaaat ctatttcaac
     8281 ttcttcatac acatttcctt tttctgtatt agaagtaatt gccattactt tatctcttat
     8341 agataacttt ataagattat ttttttctga ttgagacaat aaagacgctc tctctatact
     8401 atttaaaagt tccttagtat ttaatttaac tctgctgtta tgttctcttg gcaataattt
     8461 tttataatca ataaaatctc cttcaagcaa tcttgtaata attttcgtat cattaattat
     8521 aaaaatagca tttttttcat taaatccaac tttaacatta tcctctccag aaagcaaact
     8581 atttacatct ataagtgtct ttccaggaat tatgactttt atattttcag ttaaactatc
     8641 aaccgaacaa ctttttacag ctaatctata tccatcaatt gctactaaat ttaagtttct
     8701 atctacaatt tctaaaagtt ctcccattaa aacaggtttt gtttggtcct gtgagatagc
     8761 aaatacagtt tgtttaatca tattttttag gatttcttga ggtatgctat ataaatcttc
     8821 ttcatttaat tctggtaatt taggaaattc ttttgctgca taacctttaa ttttaaatct
     8881 tgaatttaca caatttatgt aaatattatt ttctgaatca gtttctatct caacaaagga
     8941 atctggtaat tttctaatta tatctccaaa tagccttgca tctacaacaa catcaccttt
     9001 ttctatgatt tctgcttgta cataagtttc tattcctatt tcagcatcat atccagtaag
     9061 ttttaattga ccttcttctg tagatataag tatacctttt aataattcta ttgttgtttt
     9121 tccattaata gctttttgag caattccaat tctatttgct aggatttttt gattacaaat
     9181 tattttcaat gtggataacc tcctctggct ttttatatat aaaaataagt taaaatcata
     9241 gtagtaatag tagtaggtgt tgttgataat gttaataagt atgtataaca ttataaatac
     9301 aaagatatta acatgtgtat aagatgttaa tatctttgtt attttagact tgataatttg
     9361 ttaataattt ttgttcaaaa aaagtattaa caggttattc aagagatata aacatacaat
     9421 tgtgtataaa ttatcccttc aaatctgata taattttgtc tattttagtc ttaatttctt
     9481 cactttcttc tatatcctta gatactttgt catgagcatg aattactgtt gtatggtctc
     9541 tacccccaaa ctcttcacct attttaggaa gggataaatc agtaagctcg cgcgttaaat
     9601 acatagctat ctgtcttgga taagctattg aacgtgttct ttttttagaa ttaaaatctt
     9661 ccatctttaa attgaataca gaagatactt tttcttttat tctgagaaca tttatttctt
     9721 catttttaga agttgttata atatctttta aagcttctgt agcaagatca aaagatatta
     9781 ccctattgct aagagaagaa taagcaacca ctctagttaa tgcaccttct agttccctta
     9841 tgtttgactt aatatttttt gctatatatg acataacctc atttggcaca tctatacgtt
     9901 ctagttgagc cttttttcta agtatagcaa ttcttgtctc aaagtcaggt gcttgaatat
     9961 cagttataag tcccatttca aatctcgatc ttagcctatc ttctagtgta ggtatgtctt
    10021 ttggcggtct atcactagat ataattattt gtttgtttgc ttcatgaaga gtattgaatg
    10081 tatggaaaaa ctcttcttga gttctttctt ttcctgctat aaattgaata tcgtcgatta
    10141 gtaatacatc tacgtttcta tatttattcc taaattcttc gtttttatcg tcttttatag
    10201 agtttataag ttcattagta aatttttctg aagaaacgta aactactttt gagtcttttt
    10261 tttgactaac aatatgatga cctatagcat gcattaggtg agtcttacct aatccaactc
    10321 ctccatacaa aaaaagtggg ttataagctt tagctggaga ttctgcaact gcaacgcatg
    10381 cagcatgtgc aaatctatta ctattaccaa taacgaatgt atcaaatgtg tattttggat
    10441 ttagattggg gtaaagaact ctataattta aatctgtgct atcagaattt aaatcagcaa
    10501 cctctttttc gctcaataca aatttaatat tatatttttt taatgacagt tgactaatag
    10561 catcttcaat taaatgtaaa tatctattct ctaagatatc tttattaaaa tcactaggag
    10621 ccagtaagat taaatcatta agatgtattt ttaatggtac gatgttttta aaaaaggtat
    10681 taaaacttac tgaagttaag tcacctttta ttaattgtag ggttttgtcc cataaagaaa
    10741 ctatatccat aatataaacc tccgaacaga ataattaata ttttccacaa agttattaac
    10801 agaaaagcaa aaatatatat aaattttaca gaataactaa atttatggat aatggtgttg
    10861 ataaattaat aatagtataa attattaaaa aaatatgaaa agatataaac aatatgtaga
    10921 taaagttata aataacataa acatacaata aatggtgatt ataactagag tattgtcagt
    10981 aatcttaaaa gttatacaca aaagtatcca caagctgtga ataatttcta ataagttgat
    11041 aacatttatg cacattatca acaagtatat ttataatatc aaaatatatg tcaaacttca
    11101 atgatttgta actaatttat ccacaatatt aactaattgt tgataaaaat ataaacatgt
    11161 taaaattact ttatttgtgt atataattga aaaaaataat ttagaataaa attaaataga
    11221 tttttactag tattttcaat aaaaaaaata cataatatat atatggtaga ttttgtgata
    11281 taagcgtccc aaaaactcgt tgacaacagt agtaaaagta tttataatta tatgtgttat
    11341 gtttactttg agggtaaagt tattattttt ataaacggaa aataaaatct gaaaggcggt
    11401 gcaaaataat gagtaaaaga acttatcaac caaaaaagag acaaagaagt aaagaacatg
    11461 gtttcagaaa aagaatgaag acgtctaacg gaagaaacgt gttaaagaga agaagagcta
    11521 aaggaagaaa tagattaact cattaattac aaaagggccg ctgcaaaggt ggcctttttg
    11581 tgtaaataat tacaattcat agctgtgttt atggaggtta actatggact ttaataggac
    11641 taaagggttg aaaaaagact ctgattttag aaaagtatat aaacacggca aatcttttgc
    11701 aaacaaatat ttagtaatat atatacttaa aaataaatca gattatagta gagtaggtat
    11761 ttctgtttca aaaaaagtag gaaaagctat tactaggaat agagtaagaa gattaataaa
    11821 agaagcctac agattaaata ttgatgaaaa aattaagcct ggatatgata tagtatttat
    11881 agcaagggta tctagtaagg atgctacttt taaagacata gacaagtcta taaaaaattt
    11941 agttaaaaga acggatattt ctatttaatt gtatgctata aatatggagg ttaatgactt
    12001 tgaataaaat aatttatata ttaaaggaaa taagtaagta tttatcgaat ttatgtattt
    12061 atttagtgag attttatcaa aagtatatat ctcccttaaa gggacctacc tgtagatttt
    12121 atccaacttg ctcacaatat tcaatagagg cgtttaagaa atatggattt gtaaaaggta
    12181 tgtatttaac gataagaaga attttaaaat gtcatccttt tcatccagga ggttacgacc
    12241 ctctaaaata aaagagagta actattagga ggtataaaaa aattgaatat aataggaaat
    12301 ttcttaggat tgattcttaa ggtaatattt gaatttgtta atcattatgg tatttctata
    12361 atacttttta cgatacttgt aaagattatt ttattaccat taacaattaa gcagactaag
    12421 tcaacaaagg caatgcaaga tatacaacct cgtataaaag agatacaaga aaaatataaa
    12481 aataagccag aaaaacaaaa tgaagaaatt gttaagttat atggagaagc taaaattaat
    12541 ccactatctg gttgtttacc tttattaata cagtttccta tattaatagg attatttagc
    12601 gttttgagag aaccagttgc tcatggcgtt tttgcaaaca aagctgcatt tttagcagct
    12661 gacaatgggt ttttatggat aaaaagctta acatcaccag attatgtatt agcagtattt
    12721 tcaggagcaa gtgcatatgt catgcaaaaa gtaatgacac caaaagatca gttacaaggc
    12781 tctatgaaag taatgacata tgtaatggca ggtatgtctt tctattgggg atttatattc
    12841 ccagcaggac ttacattgta ctggacagta agtaacttat tctcaatagc tcaatattac
    12901 ttaataatga atcctttaaa agctaaactg gctgctaact ctaaggagga aaaagttaat
    12961 gagaaaaagc ctaaaattaa taaaaagtaa gagtaaagag gaagctataa gtaaagctat
    13021 agcagagttg aatttaaaag cagaggatat agaagtagaa atacttgaaa atccaagtaa
    13081 agggttttta ggttttatag gtgctaaaga tggtacttat gaaatttttg ttatcgagag
    13141 ggaagtagat gtagctaaaa attttataga ggtaatgttg aaaaatgcaa atgttgatgc
    13201 caaagtcaat gtatctcaaa aagataattt aatcatggtt gatattgaag gcaaagaagc
    13261 tgcaagttta attggtagaa gaggggaaac tttagattct attcaatttt tgactggatt
    13321 agctcttaat aaaataaata aagattctca tacaagagta cttgtagata tagaaaatta
    13381 tagatcaaaa cgagaagaat ctttaataag atatgctaat aaagttgcta gagaagtagc
    13441 gaaaacaaga aaaactaaaa aattagatta tatgaatcca tatgagagaa gaataataca
    13501 ttcagctctt caaaatgaca aatatgtaat tacatatagt gaaggaaccg atccatatag
    13561 aagattagta attgagtgta aaagataaag tcaacctcct aaacattagg gggttttatt
    13621 ttttaatatt attaaatcat taatgattgg ttaaatgtaa atctatatgt aaaaaattat
    13681 gaattaagag gtgataaatt tgtttataga tgatacaatt gcagcaatag ctacagcacc
    13741 tggagaaggt ggtataggta tacttagaat aagtggagaa aaagctttga aggttgcaga
    13801 agaaatcttt aaatctatgt ctggaaagag tattgaagaa tacaataaga gaacattaat
    13861 atatggaaat atagtggata atgaaaatat aattgatgaa gttttattgg catatatgaa
    13921 aggaccaaat tcctatacag gagaagatgt tatagaaatt aattgccatg gtggatttat
    13981 ttcggtaaag aagatattag aattaatact atcaaaagat gttagattag cagaggctgg
    14041 tgagtttact aaaagagcat tcttaaatgg aagaatagat ttatctcaag cagaggctgt
    14101 aattgatgtt ataaaagcca aaactgatat agctcatgaa gttgctcaaa atcaattaga
    14161 aggttcttta tcaaagaaga taagagaatt gagagataaa gtcactgaga tattagctca
    14221 tgtagaagtt gctatagatt atcctgagga agatattgag catataacat atcaaactct
    14281 taaagaaaaa actgatgaac ttaaaaaaga cattaaaaaa ttatatgata cagctgaaag
    14341 tggaaaaata cttagagaag gcttaaagac ggtaattgtt gggaaaccaa atgtaggtaa
    14401 atcttcacta cttaattcta ttttaggaga gaatagagca atagttactg atataccagg
    14461 aactactaga gatgttatag aagagtttgt aaacataaaa ggtatacctt taaaaattgt
    14521 tgacacagca ggtataagag atactgatga tattgttgaa aaaataggtg ttgaaaagtc
    14581 taaagaatca tttactagtg cagatttgat agtaatggtt ttagatgcat ctagaaagtt
    14641 gtcagaagaa gatatagaga tattagaaaa acttaaggat aaacaaacaa tagtgctgtt
    14701 aaataagaat gatttgaaac aagagattga agaagaaaaa atacttaaat atgtagaaaa
    14761 taatagtata ataaaaatat cagctttaca acaagaagga atagaagaat tgcaagataa
    14821 aatagagtct atggtatata aaggtagtat caaaaataat tctagtttag tagtaacaaa
    14881 ttcaagacat aaagatgcac ttagtaaagc atataaatct gcaacagatg ctttaattgc
    14941 tttggaacaa agtatgccat ttgactttgt agaagtcgat ttaaaaaata tatgggacta
    15001 tcttggatat attaatggtg atacagtaac agaggattta ctagataata tattccataa
    15061 tttctgtatt ggtaagtaat gtacaaaagt aaacttgttt ttattataga gatttttaaa
    15121 aaatttgaag tgtttctcat gaaacattaa ttttgaaaag aaaggaatta taaatgataa
    15181 agtttgaagc aggaaaatat gatgttatag tagtcggagc aggacatgct ggatgtgaag
    15241 ctgctttggc tactgcaaga atgggatata aaactttgat aataacaatg tctttggatt
    15301 ccatagcatt aatgccttgt aatccatcaa ttggagggac tggtaaaggt caattagtta
    15361 aagaaataga tgccttaggt ggtcagatgg gattaaatat agacaagaca tatatacaaa
    15421 gtagaatgtt aaatactgcc aaaggtccag cggtacactc tttaagagca caagcagata
    15481 aatttaaata tcatgaagaa atgaaaaaga cattagaaga tgaaccaaat cttgatatag
    15541 ctatggatga agttgtagaa atacttcatg aaggaaatgt tgtaatagga gttggaacta
    15601 aactaggatg ttcatttaaa tctaaagctg taatattggc cactggagtt tatctgaact
    15661 caaagatata tatgggtgaa gttgcatttt atgaaggtcc taatgcattg gggtatgcaa
    15721 aatatcttac agatagctta gttgagcttg gccttagaat gagaagattt aagacgggaa
    15781 ctccagcaag agttcataga gatagtatag acttctctgt aatgtcttta caagagggtg
    15841 atgaaaaagt aactccattc tcatttatga atgaaaatat agaaaaaaaa caagaacctt
    15901 gttatttaac aagaactaca gaagaaactc aaaaagttat actggataat ttaaaaaggt
    15961 ctgctatgta tagtggagta atagaaagta ctggtccaag atactgtcct tctatagaag
    16021 ataaagtagt aagatttagt gataaaacat cacatcaatt atttatagaa ccagaagggt
    16081 taaatactaa agaaatgtat atacaaggaa tttcaactag tttacctttt gaagttcaat
    16141 tagatatgta caaaactata aagggacttg aaaattgtaa gataatgaga cctgcatatg
    16201 ctattgaata tgattgtgtt gacccaactc aactaaaaat aagtttggaa ataaagggtg
    16261 ttgagaactt atttagtgca gggcaattta atggaacttc tggatacgag gaagcagcag
    16321 ctcaagggct tatggctggt ataaatgcag ttagaaagat agaaggaaaa gagccatttg
    16381 tattagaccg ttcagaagct tatataggtg tcttattaga tgaccttgtt actaaaggaa
    16441 ctaacgagcc ttacagaatg atgacatcaa gagcagaata tcgactatat ttaagacaag
    16501 ataatgcaga tatgagatta actcaaaaag gttatgatat aggattagtt aaaaaagata
    16561 gatatgaaag gttcctaaat aaaaaggctg ctgtagagaa agaatttgaa agactaaaaa
    16621 atgaaagagt tacaccaaaa gaagtaaata gtttgttaga agaaaaagga gcaacaccaa
    16681 taaaggttgg aatatctcta tatgaatttt taaagagacc agaggttact tacgaattgc
    16741 ttgaagaatt agggaaaggc gctggagaag atgtttcaag agaagttaaa gaacagtgtg
    16801 taataataac taaatatgaa ggttatatag agaaacaatt gaaacaaata gaccaattta
    16861 aaaaacttga gaataaaaag ctggatgaga aaataaatta ttcatctata gaaggtctta
    16921 gattagaggc tagacagaaa ctagatgata taaaacctat ttcaataggg caagcatctc
    16981 gtatatctgg tgtttctcca gctgatatat cagttttact tatataccta gaacaaatca
    17041 gaagaactag aggtggcaaa ggtgaataat atagaacttc tgaaaaacgg gattgaaggt
    17101 tttaatatag atacgaatga cagtatgtta gaaaaattta aaatctatag agagatatta
    17161 gttgactgga ataaaaagat gaatcttact ggaatagaag atgaaaaaga agtgtatata
    17221 aaacattttt tagactctat ttcagctgta aagaatggat atataaaaaa tgggatgtct
    17281 ataatagatg ttggtacagg tgctggtttt cctggaatac cattgaaaat atgtttagat
    17341 agcttagaac ttactttgtt agattcgtta aataaaagaa taaacttttt agaggaagtt
    17401 agtagagtac tagagttaga taatattaca tttatacatg gaagagctga agattttggt
    17461 aaagatgaag actataggga aaaatatgat gttgctacgg caagagctgt tgctggatta
    17521 ccgattctta tggaattttg tgtaccattt gtaaaagttg gagggtattt tatttgctta
    17581 aaaggtccga atgctaattt agagctagaa gaatcacaaa aggctattga tgttttagga
    17641 ttggagtata ttgaaaaaat agatgtagag ttaccagagg tagatttaaa tcataatata
    17701 ttagttttta aaaaagtgat ggaaacacca gttaagtacc ctagaaaagc tggtaaacca
    17761 gccaaaaatc ctattaagta atgaaaaatt tattattggt aagcttaatt atttctcagg
    17821 aaataattaa gcttactgta aaaaaacaaa aataaaggaa ttattaaagt agatgcagaa
    17881 ataaatttgt atgtacatat agagaacaaa aataatttat atctaaaata agagaggtat
    17941 gttatggaag ataaaagagt tatggagata cctatagaag atgtagttcc aaatccatat
    18001 caaccaagaa aaatattttc acaagtttca ttggaggaat tgagcaattc tataaaggta
    18061 tatggtataa ttcaacctat aacagttaga gcaaaagatg gcaaatatga actaattgca
    18121 ggagaaagaa gattaagagc agctaaatta gcagagctta aaaccatacc agcaattata
    18181 aataatatga acgatgaatc ttctgctgtt ctagcgttat tagaaaattt gcaacgagaa
    18241 gacttaaatt tcattgaaga agcaattggt tatgaaaact taataaaaga acatgctttt
    18301 actcaacagc aattagcaga aaaattgggc aaaaatcaat ctactgtagc aaataagctt
    18361 agaatattaa agcttccaaa cgatataaaa atgaagctaa tagagaataa tttaacagaa
    18421 agacatgcta gagcatttct taaactacca agtgaagatt taatgcaaag tgtgttagac
    18481 aaagtcataa aaaatgaatt gactgttaaa aaaacagaaa aattgataca agatgtatta
    18541 gaagaaacta aagttcaaga agagcctgat aaaaaacaga atataaaggg tgctatgagt
    18601 ataagaattt acataaatac tataaagcag gcctttgatg ctatatcaaa tacaggaatt
    18661 gatgctaagt ataatgaaat agataaggga gattatatgg aagttgttgt aaaaatccct
    18721 aaaaaataga gattactgct taccagtaat ctttttttgt aatataagcc ttgtaatata
    18781 aattgattta tagacattat ttatacttaa aaaacaccta ttcaacattt aaaattaatt
    18841 taattcttaa aggggggaac atcatgggca aagttatagc tgtattcaat caaaagggtg
    18901 gagtcgggaa aactacaaca aatgtaaatt taagtgctag cttaggaact ttgggaaaga
    18961 aaatattagt attagattta gacccacaag gaaatacaac tagtggatat ggtattaata
    19021 aaaacgaagt agaaaataca atctatgaaa ttatgttaga tgggttacat attaaagaag
    19081 caataataag tactgaattt gagaatgtag atgtagttcc atcagctact gaactatcag
    19141 gtgcagagat agagcttaca tctaaaacta atagagagta tattttaaaa aattccataa
    19201 aagcggtaat agatgaatat gattatatat ttttagattg tccaccctca ctggggatgc
    19261 ttacaataaa ttgtttaact gctgtagata gtgttttaat tccaatacaa tgtgaatatt
    19321 atgctttaga aggtgttagt caattaatgg agactataaa attagtaaaa tcaagattaa
    19381 atgctgatat agaaattcaa ggtgttgttt taagtatgtt tgatggaaga gctaatttat
    19441 caatacaagt agttgaagaa gttaagaaat actttaaagg tagtgtatat acaactttaa
    19501 ttccaagaaa tgtaagactt gcagaagctc caagtcatgg aaaacctgta atttattatg
    19561 ataaaagatg tagagggtct gtagcttatt tggagctagc agaagaattt atagatttag
    19621 aagaggagga atggtagtat ggagaataag tctaaaagaa gtaatagatt aggtagaggt
    19681 cttagtgcat tgataccaga aattaaagga gaaacttcag aaaaagaaat agtcaacata
    19741 gatatagaca aaatatatcc aaacgaagtt caacctagaa agcaatttga tgaagaaaag
    19801 ataaaagtat tgtcggattc tataaaaaat tatggtgttt tacagccaat agtggttaaa
    19861 atggatgaaa ataataaata tatgataata gctggagaaa gaagatttag agcatcaaaa
    19921 cttgcaaata aaaatcaaat acctgctata attaaagata tagatatgaa agatataatg
    19981 gagatagcct taattgaaaa cttacagagg gaagatttaa attcaataga agaggctcta
    20041 gcatataaga gtttaataga gcattacaat gtaactcagg aagaaatatc agaagctgtt
    20101 gggaagagta gacctcatat aactaataca ctaagacttt taaatcttgg acaagatgtt
    20161 atagaaatga tagacagtgg tagaatcaca gcaggccatg gtaaggcttt attgagaatt
    20221 gcagataaag atttgcaact tcaaatagcg aaaaaaatag aagaagaaga actttctgta
    20281 agagaagtcg aaaatatagc taaaaaaata tctgaaaata aacaagaaga acctaaaaaa
    20341 tctaaaccta aggatgtatt tattttagat gttgaagata aacttagaaa tatatttgga
    20401 acaaaggtga atatttctaa aggaaagaaa aaaggtaaaa tagagataga atactataat
    20461 gatgatgatt taaatagtat tgtatcaatg cttcttgaat agaacaaaaa tataaaacag
    20521 agaggaagat tgttatgatt tatttagata atgctgcaac tacatatcca aaaccagaaa
    20581 gagtgtataa tgctgtttta gattgtatga aaaattattg tgcaaatcca ggtagagcag
    20641 gccataaact tgctatgaga gctgctaggg agatatatga tacaagagag aatatagcta
    20701 agttgttcaa tgtaagtaat cctatgaata tagtatttac atctaatgca acagattctt
    20761 taaatttagc gataaaaggt gtattacaag aaggagacca tgtaataaca acgtctatgg
    20821 agcataattc agtaataaga cctataaaag cattggaaaa aaggggtatt gaaaatactg
    20881 tagtaaaatg cgattatgag ggatttttag attatgaaga tttagaaaaa tcaataaaga
    20941 gtaatactaa actgattgta acaacacatg cgtctaatgt atgtggaact ttaatagata
    21001 taaaaaaagt tggagaaata gctaaaaaac ataatatact atttttagta gatgcatcac
    21061 aaacagcagg tgtttatgat atagacgtaa atgaatgtaa tatagatatg ttggcaatgc
    21121 caggtcataa atgcttattt ggacctcaag gaacaggtat attatatgta agagaagggt
    21181 tgaacttaaa tatattaaaa gaaggtggaa caggtagtaa atcagaagaa atagttcaac
    21241 cagagttatt cccagataaa tatgaatcag gaactcataa tactccaggt atagcaggtc
    21301 ttaaccaagg tatactattt atatttgaaa gaggaatcaa caatataaga caacatgaag
    21361 aagaattatg tcaatatatg atagacaaat tagaggaagt accagatata aagatatatg
    21421 gaccaaagga cagtaaaaaa agagcatcag taatagctct aaatatagga gatatggatt
    21481 ctggagaggt tactttctta ttagatagtg attataatat agcgactaga tcaggaatac
    21541 attgttcacc actagcacat accactttag gtactttaaa acaaggtgca gtaaggttta
    21601 gtataggata tttcaacaca aaagatgaga tagataaagc agtggaagct ttgaaaaaaa
    21661 tttcaaaaaa taaatagcat gagatttaat aatatagaaa taaaaaaagg ggatatttca
    21721 aaataaaaat ttgaatcatc tccttttttg tacaaaaaag aaaaggggtt cctaagaacc
    21781 tctaatctat ttgataatag tttcaagaaa ttactttaag taatctcctt cttcaagaac
    21841 tatatgacca ctttcgtaat tagaaccatc aacagtaatt tttatttgtt caacattcat
    21901 attttttatg taagtacgag caactgaatc aagcattaaa gtttctgcat cactacctaa
    21961 gtttgacttt gtaaagtcag agctaacatc taatatacca gtttttacac catcaatgtc
    22021 ttttgtagta aatgaattta attttgcaga ttctggaaca actttaagtt tttgaagttc
    22081 ttcaaataaa gtgttctcat caattttatt taaatcaact ttattttcaa ttaaatctgt
    22141 cttatctaca tcaaaagagt aaatagtagc tgattgagtt ttagtttctt cttttggagt
    22201 aggtttagct ttgttattag gaacctcttt ttcagtattg ttttcttctt ttggttgctc
    22261 aacatttgta ttgctcttag tttctttttt agatgaatca cttccatttt gagcattagt
    22321 acaaccaact gcgaatatag atatagataa tatacttaac actaacatta ttttcttatt
    22381 tttcataaaa atttctcctc tcttaaaaac tattcttata tataattata gtcgttatga
    22441 gcaaatatat gaatacaaaa tagtaacaaa tttaaaaaat gatttattta aattaataaa
    22501 actcaaaata aactaaattt tagtcatttt tatgggaaat catttaatgt acagtgaaaa
    22561 tattgtaaaa taaaagtagt gtttttaaaa taaatataag ttataattat attataaaaa
    22621 aatgtcttca aaaggtggga atagtatgga aattttgagc ttaggagaaa agattaaaaa
    22681 attaagaaaa gaaaaaaaca tgacattaaa agaattagct ggagatagga taacagcagc
    22741 acaaataagc catatagaga gggataaatc tcatactagt tatgaactgt tggaatatct
    22801 aggcgataag ctagatgtaa gtgtagaata cttattagag accaaggaaa tgcaatcaaa
    22861 aaagataact gataatttaa tactacagag cgaaatttat ataaaagaaa atgaattaaa
    22921 caaggctgag gaccaaataa aagaagtttt agatatatgt aagagatacg atttaatgga
    22981 gaactatggc agatgtaatt ttttaatggc taatattaat ttgaaaaaat ctaattataa
    23041 tgatgctata gaaaactttg aaaaggcatt gtacttcttt ataaaaaata gtgatagtga
    23101 aaatatactt aagtgttatt taaacatagg gaaaatatat atgcaggaag acttttataa
    23161 aggtgctata agccacttta attttgcaga ggaaattttg tcagagaata aatttgagga
    23221 tgttaacata cacaaggaac tatatagtaa gatggcatat tgttacataa aactagataa
    23281 tccaaatatg tcattgaagt acatagaaaa gataaatgaa atagataata gaaatgatag
    23341 aaaagaagat gtcgatattt tggtgttaaa agcaaataat atgcttgaga ttggaaaata
    23401 tgaagaatca agagattact ttaaaaaagc actaaaaata cttgagagtg aggataataa
    23461 aactggatta gcaaatgtgt atttaacaat ttgtgatgtt tacagaaaaa taggtgaaac
    23521 agatagagta cttgaatatt cacaaaaggt atatgacata aagaaaaatg atgaagatga
    23581 atacatgatg tcaggattat ttaagataat agaagcatat atagataaag aagattatga
    23641 tttagctaga aaatattgta agatagcctt agcttcatca ataaaaaata aaaataaatt
    23701 caatgaatat aaagcattga gattctatgc taatatgtat aaaaatcaga atgaagtaac
    23761 tcttgcaatt gaatatctga ttaaatgcat aaaaatagta tcagatttag gcaacaatag
    23821 aatattggcc aatttatata tagacttagg tcaattatat tcaaatatat caaaagaaaa
    23881 agagttagaa tattatcaaa aaggtgtatt tatgtacaaa aatttagaaa taatgtaggg
    23941 taaataaacc tataagggta tagaaacaat ctataacaag caagataata tctatgataa
    24001 aatataaatt gtatgaatat ttttaaggag atgaatcaga caatgagtat aaaaatagat
    24061 gcgagagggc ttgcttgtcc taaaccagtt ataaatacaa aaagagaatt ggataaccta
    24121 gaagaaggtg ttgtagttac aatagtcgat aatgaaacag ctaaggagaa tatattaaaa
    24181 cttgctaaat ctttaagttg tgaagcaaat attgttgacg aaaaagagaa ttttatatct
    24241 atagaaatta gaaaaggtaa aaatatacta gataaacaaa atgttgtaga taataaagaa
    24301 agtgaattag acaatacatg tatatttata tcttcagata agatgggact tggaaatgac
    24361 gaattaggaa aagtgttaat aaaaggattt atatatacat taacagaatc taaaccttat
    24421 cctaagtatg tacttttagt taatggagga gtaaaattaa gtgctgaaaa tgaagaaacc
    24481 atagaaaatc taaaaatact tgagaatatg ggtgtagaag ttttatcttg tggtacttgt
    24541 ttagattatt ataatttaaa agacaaatta caagtaggta cagttacaaa tatgtatact
    24601 atagttgaga cattaaagaa tgcatcaaat accatttcta tataatgtat gtttgtttaa
    24661 taatataaaa ttaataataa agattaactc aaatagatag tcagaaagga ttaagtaatg
    24721 aatcaaatgt atatagtgtc atttaattcg acacatcatg cgataagatc agaaaagtta
    24781 tttggtgaaa atagtctaaa agtaatggct cttccaacac caagagaaat aacagcaagt
    24841 tgtggaatat caattaaatt ttcttttgaa gatatggaaa agataaaaac tattttagtt
    24901 gaaaataatg tagatataaa aggaatttat tgtataagta aattagaaaa tggctcaaaa
    24961 gaagtagaga agttagatta ggaggctaat tatttatgcc aatggattta aaaataggtg
    25021 atattgttga attaaaaaag caacatgcat gtgggtgtaa agagtttgaa atcataagaa
    25081 cgggaatgga tataaagata aaatgtacta aatgttcaag gctaataatg cttgatagag
    25141 agaccttaga aaagagagta aaaaagctaa taaaaaagtg ataaaaattt aacacttaaa
    25201 aaatgtcaaa taccgtatat taatatccaa aattttggta taataagaat tgaagaaaac
    25261 attttcatgg gggtgcttta attatggcag ttaaatatgc aaaaagaatg caaggattac
    25321 aaggatcaga aatacgtgag cttttaaaac ttactcaaca accacaaata atatcttttg
    25381 ctggtggaat gccggctcca gagttatttc cagttgagga aatgaaaaaa gtatcagttg
    25441 cagtgcttga agaaaatgga agatcagcta tgcaatatac tacaacagaa ggatatgaac
    25501 cattaagaga aaaaatagca gctagaatga atgataaaaa caaaactaat gtaaacaaag
    25561 atgatatatt agttacaagt ggttctcaac aaggtctaga ttttgcagga aaagtattta
    25621 ttgatgaggg tgatgtaatt ttatgtgaaa gtccatctta cataggagct ataaacgcat
    25681 ttaaatctta tcaacctaaa ttcatagatg ttccaacaga ttctgatgga atgataatgg
    25741 aagaattaga aaagatacta gaaacaactg atagaataaa aatgatttat gtaatacctg
    25801 acttccaaaa tccaacagga agaacttggc cacttgaaag acgtaaaaaa ttcatggaaa
    25861 tagtaaataa atttgaaata ccagtaatag aagataatcc atatggagat ttaagatttg
    25921 aaggagagac tttaccatct ttaaaatcaa tggatacaaa aggattagta atattcttag
    25981 gaactttctc taaaatattc tgcccaggat acagattagg atggacttgt gcttctccag
    26041 aaattttatc taagtttaac tttgcaaaac aaggtgcaga cttacaagca tcaactatat
    26101 ctcaaatgga agtaagcaaa tttatggata tgtatgacct agatgcacat gttgacaaaa
    26161 taaaagcagt ttatgttaaa cgtagagatg taatgcttaa gactatggaa gaagagttcc
    26221 cagaaggatt agtatttact catccagaag gtggattatt tacatgggta gagcttccaa
    26281 gtaatttaaa tgctaaagaa ttaatgccaa aatgtttaga taagaatgtt gcttatgttg
    26341 ctggaggagg attcttccct aatggtggta gagaaaatac tttcagactt aactattcaa
    26401 atatgccaga agaaaaaata atagaaggta taaaaaatat agcagctgta ttaaaagaag
    26461 ctatgggtgt agaagcttaa ttaaaaataa taacttatat ataatacaag ggatggttgt
    26521 tgccatccct tgtttgtttg aaaagtatat aattcacctt atactaaact ttgtccaaaa
    26581 tttaaattga cagaaataag aataaatgat aaaatgtgta ggtatgagat tttagtctca
    26641 tttctctgct ctaagagtgt agagccgtta agtccaaagg gaggtgaatt atagtgagaa
    26701 attatgaatt agtttatgtg gtaaagccaa actctgatga agaagtaaga gaggctatac
    26761 taaacaaagt taaggaagtt gttgcaactg acggagaaat agttaaagtt gatacttggg
    26821 gaactaaaaa attagcttat ccaatagcta agtttacaga aggtttctat gtattagtta
    26881 acttcaaatc agcagtagac gttcctaaag agatagacag aaacttaaag ataaatgaaa
    26941 acgtaataag acacatgata gttgttgctt aataactggt tttattataa aaaataggtg
    27001 gtgtttttat gaatcaagtt gtattagtgg gaaggttgac tagagaccct gaattaagat
    27061 acatcccagg tacaggaaca ccagtggcat cctttacaat agccattgat agagactatg
    27121 taaaaaaaga tggatctaaa gaaactgatt ttatacctgt tgaagtaatg ggtaaatctg
    27181 ctgaattttg tgctaactac ataactaaag gtagattagt agcacttcaa ggatctataa
    27241 gagtagacaa ttatcagaca caatcaggtg aaaagagaac ttttacaaaa gtaagtacta
    27301 gatcagtaca agctttggat agtaagaata aatcggaaaa ttcatataga gagagtgctc
    27361 cagcttttga gccaagcttt gaacctcaag gtttagaccc acaaggtttc caagctatag
    27421 atgatgacga tataccattt taaaaaggag ggaaaaaagt catgatgaat aaaaaaagac
    27481 gtaagaaaaa aagagtttgt caattttgtg ctgacaaaaa cgctaaaata gattacaaaa
    27541 gtactcaaag attacaaaag tatataactg aaagaggaaa gatattacca agaagaatat
    27601 ctggaacttg tgctaagcat caaagagaat taacagttgc tataaaaaga gcgagaaata
    27661 tagcactttt accatataca ttagactaaa aaaatgagcc ttgtgctcat tttttattat
    27721 atgtatttat atatctaaat gaggcgagct ataaagctcg tttttttgtt tatatactta
    27781 aatatattat tatttatgat ataatattat atattaagga gggttgtaaa cgtgaaaata
    27841 gataaaggtt ctgagataac gagaaacata aagataatag aatggatgaa aacagaaata
    27901 ttaatgagtg taagtgattt gtttaattta ttgtttaaag gggtaaaacc attagatgaa
    27961 gcaattcaag atactttagc aaatataata atgataacat atctcttagc aaagagatta
    28021 ggaattagtt ttagagatgt ggattataaa gtcaaggaaa aaataaaaat tggtatagat
    28081 gaggaccata gcgttgaaag atggtatgga gatttatcta atttaaaaaa acatatggat
    28141 aatagggagt gatattaaat tgaataataa aattagacta tctaaggcaa tgtctataat
    28201 agttttagct ataataatcg ctttagtaat agcctatatt ccaatgctag gaatatttag
    28261 tatgttagca gctgttccat atgtaattat aggtgctatt actgatagaa agtattctct
    28321 tatatctatt ttggttacat tttgtgtact gattctattt gcagacattt cttatgcatt
    28381 aaatatatgt ataatgtatt ctttaccagg tatagtaata ggaaagatgc taaagagaag
    28441 ctttgaacaa gaggacagta ataaattcga accgatatat ggtggaacca taatatttgt
    28501 actttcaatg cttgtatatt tttttgtatt aaagacattt ttaaatgtga atctattaga
    28561 tgaagtttct aagatgatat cagaaattgt aaatattcaa aaaaatagtt tttctgcaac
    28621 tgaacttaaa gtgtttgata aaatgaagcc agatgagatt gtaagttatt ttacaaatat
    28681 gctaccaatg atgttatttt tacaagggtt gttatcagcg tttgtcacat attatttgag
    28741 tgtatttttt ataaaaagga ttacaaagat gaatataaga tttcctaagt ttgctgattt
    28801 ttatttaccg ggaaatgcta tactcacaac tttcttactt tatttattag tcttatttat
    28861 tcaaattatt ggttcaaaat tatatacaga attaataatg actaatttac agctagtttt
    28921 taatttaatg tttgtaattc aaggtatagc agtttgcatt tatttcttaa agaaatggat
    28981 aagacaggga ccaaataaaa ttatgttttt ttcagggata attttatgtt tatttggatt
    29041 tatgggaata tcttttgttg gtatggtaga tagtataatt gactttagaa aggtaagaag
    29101 ctataaatcc acttaggagg atagaaatga gtaataaaca aacctttaaa ttaaatatgc
    29161 cagaaataaa tttatatata attgttattg gtatttctag tataattttg ctttattaca
    29221 acttgtatgt agggtgttta tttttctgta tttttgtata tatggttttt cacaattgga
    29281 gaactactaa tattagacgt catgaatgga cagagtatat tcaaaattta tctttagata
    29341 tagatgaaac aactaaaaaa gccataataa atcttccaat accattatgt attttagaat
    29401 ttgatggaaa tatatcctgg tataatggaa agttttatga tatgattggt caaaaagatt
    29461 tacttgataa aaatatagag gatatagtta aaaatcttaa tttaagaaaa gtattaaatg
    29521 aaaataaaga gatgtacaca gaaataaact ataaagaaaa agaatataca ataatttata
    29581 atgttataaa aaatgatcaa gaaaaaaatc ctaaatattt gatgatatta tactggatag
    29641 acaagactga atatttaaaa gtaaaacaaa attatgatga tgaaaaaaat gcaatgatgc
    29701 taatacaagt agatggatat gatgaagtat taaaaagtgc agcagaagat aagagagctt
    29761 taattaatgt tgaagtagag aaaatacttt ctgctttaga attaaattca aatggtgcat
    29821 tgagaagaac ttcaaaagac aaattcttct tggtaatgca taaaaaagag ttaaaaaaat
    29881 tggaagctga gaagttttct attttagata caataagaca tattgattat ggaaataatc
    29941 ttcctgttac tataagtata ggaattggta tagatggaga tacattaaat gagaacctaa
    30001 aacttgctac gggtgctctc gatttagcac ttggtagagg tggtgaccag gctgttgtaa
    30061 aaacaaaaga taaatttgtg ttttatggag gaaaatctaa agctgtagaa aagaaaacca
    30121 aagtaaaatc cagattaata ggtcatgctt taagagaggt tatacaacaa agtgaccaag
    30181 tttatataat gggacataaa tatcctgata tggatgctat gggagcagca gttggtgttt
    30241 atgatatatg taaatcctgt aacaaaactg caaacatagt tttacaatct gtaaatgaat
    30301 ctatagaaat atttataaat aaaataaatg aaaataatta ctataaaaag ttgtttattg
    30361 gaaaagagga agctattgac aactgcacta aaaatacatt agttgtagta gttgatactc
    30421 atagaccaaa ttatactgaa tgtgaagaac tgttaaagct atcagagaag gtagtagtta
    30481 tagaccatca tagaagaggt gtagagttta taaatgatgc agtgctttta ttccatgaga
    30541 tatatgtatc ctcaacgtgt gaaatggtta cagagttagt tcagtatatg gatgaagatg
    30601 taacaataaa taaacttaca gctgaagggc ttttagcagg tataagtcta gatactaaaa
    30661 actttgcatt caaaacaggt gttagaactt ttgaagctgc ttcttacctt agaaaagttg
    30721 gagcagatac tatagaggta aagaaattct tcaattctga tgtaaaagat ttcataataa
    30781 aagctgaaat aattcaaagt actaaaataa ttaataatag aatatgttta gcatattcaa
    30841 gtactgagat agatagtata aatgttataa tagctcaaac tgctgatgag ttattaaata
    30901 tcaaagaagt agaagcttcc tttgtattag gtgaaaaaga tgatactata tttataagtg
    30961 cgaggtcttt aggacaaata aatgtacatg tacttatgga aaaattagga ggcgggggac
    31021 acatagatat agctggagca cagttaaaaa atgtttctct aaaagaagct tataagatgg
    31081 taaataaaat aatagaggag tatttggagg aggaagaata gatgaaagtt atattattaa
    31141 aagacgtaaa aggaactggt aaaaagggag aaatgaaaga agtaagtgat ggatatgcaa
    31201 gaaatttctt atttcctaaa aagatggctg tgcaagcaga tagtgtagca ataaaagaat
    31261 taaatgaaaa gaataaatct aaagaaataa aagcacaaaa agagtatgaa gaagcagttt
    31321 tattaggtaa gcaaatggaa gaaattaata tagaaatata ttcaaaagca ggagaaggtg
    31381 gaagattatt tggttcaata acttctaaag aaattgctga gcaattaaag aaacaaaaag
    31441 acatagatgt agataaaaga aaaatattat tagatgaacc aataagatct ttaggttcaa
    31501 ctttcgtaga aataaaaata catcaaaaag taactactaa aataagagtg gatgtaaaag
    31561 aaaaacagta ataactagag gtgattaaga tggaagatat gacgagaatt cctcctcata
    31621 gtgtagaatc agaacaatca atattgggtt ctatacttct agataaagat gctataataa
    31681 cggttacaga aactataaag cctgatgatt tctataaaga ggctcacaaa ataatatatg
    31741 aatgtatgat aactttaagt aataaaggtg agcctataga cttgataaca ttaacagaag
    31801 aactaagaaa acaagggcat cttaatgata ttggaggaat cagttatatt acaagtcttt
    31861 ctactatagt accgacgact tcaaatgtaa aatactatgc tgatatagta aaagaaaagt
    31921 ctgtacttag aaagcttata aaagcttcta atgaaataat aaatctagga tatagtggag
    31981 caactaagat tgaagatgtt ttagaacaag ctgaaaagag tatatttgat atatcacagg
    32041 agaaaactag tgatgatttt aaatctataa acttagtttt gatggatgcc tatgatatga
    32101 tagaaaaatt atacactaac aagagtgatg tcactggtat aactactggt tttaaagatt
    32161 taaacaaaaa aataaatggt cttcaaagga ctgatttaat attaattgca gctagaccag
    32221 caatgggtaa aactgccttt tctttaaact tggttcaaaa tgcagcctta aaaggtgatg
    32281 cctctgtagc tgtatttagt ttagagatgt ctaaggaaca attagtacaa cgtatgttat
    32341 cttcacaatc tagtgtagaa ttaaaaaaaa taaagactgg aacacttaat gataatgatt
    32401 ggcctagaat tatagatgct atggcagtat tatcagatgc caaaatacac atagatgata
    32461 ctcctggtat aaaaatatca gaactaaggt caaagtgtag aaagttaaaa atagaaaaag
    32521 gactagattt agtgcttatt gattatcttc aacttatgga aggtgaaggt aataatgaaa
    32581 gcagacaaca agagatatct aagatatcaa ggtcactaaa aatattagca aaagaattaa
    32641 actgtcctgt agtagcacta tctcagttat cacgtgcacc agaacaaaga gcagaccata
    32701 gacctatgtt atctgattta agagagtctg gagcaataga acaggatgca gatatagtta
    32761 tgttcttata cagggacgaa tattatcatg ctgattctga gagtaaaaat ataggagaag
    32821 taataatagc aaaaaataga cacggtgaaa ctggttctgt ggaacttgtt tggcttggtg
    32881 aagttcaaag atttggggat aagttgagag acctataatc taacgaatat ccacagaacg
    32941 tcctttcttt ctacaggaaa aatgtggata catgtcacta aatatgtcca aacattgaaa
    33001 tttagctaaa aaactatcca cactatccac actatccaca gatggcctgt tgataagtgt
    33061 gaataagtag ataaagcaag cttttggctt gctttatctg tgtataaata ttaattatta
    33121 ggaggaataa tatggaaaaa ataagcttaa aatatatata tcccaatata attaaagtac
    33181 ttgatgaaat aaacttattt agagtaattg acaataattt aagagaatct attgttgttt
    33241 atgctaataa tgtggataat cagtatcata taaacatgac taatacaaac tttggaaata
    33301 taattaacat atgtaaatta gaaaaacttt tagatgtgga taaatttatg gaaaaagtta
    33361 taaaatatga aaaagaaata atagagaaag aagagttttc aaaaatagaa gaatatatgt
    33421 taaatattgg agaatactaa aaaagttgaa aaaaatacga atgtttgata gaataagtaa
    33481 gtaaagtgtt cgagagtata taaaatatag aggtgaatta catgaaaaca gttgcaattg
    33541 ttggttctca atggggagat gaaggtaaag gtaaagttat agattatctt gctactcaag
    33601 cagatgtagt agtaagaggg caaggtggaa ataatgcagg acatacatta gttgtagaag
    33661 gtaaaaaata cgcattacat ctaattccat ctggagtttt aaatccaaat acagtgaata
    33721 taatcggaaa tggaatagtg tttgacccta aaggattttt agaagaattg gaaatgttta
    33781 aaacggataa tataagtaca gaaaatataa aaataagtga tagagcacat gtcatattcc
    33841 catatcataa agaactagat gcattgtcag aagaagccag aggcgattta aagataggta
    33901 caacaaaaaa aggtattgga ccttgttata tggataaaac agagagatct ggtattagaa
    33961 tatgtgactt aatggataaa gataaatttg ctataaaatt aaaagctcaa atagatgcta
    34021 aaaatgaaat agttaaaaat atatatggaa aagaagaatt atttgatttt gaaactatat
    34081 ataacgaata tctaggatat gcagagcaaa taagaaaata tgtggcagat acgtctgtta
    34141 ttgtatatga tgcagtaaga gcaggtaaaa aagtattgtt tgaaggagca caagggactt
    34201 tattagattt agatttagga acttatccat ttgttacatc ttctcatcca acatctggtg
    34261 gatttgcaat tggtgcaggt atagggccaa atatgataaa agacgttgtt ggtatagtaa
    34321 aagcatatac tacaagagtt ggagaaggac cgtttgttac agagcaaata aatgaaactg
    34381 gagataagat aagagaacaa ggacatgaat ttggggtaac tactggtaga ccaagaagat
    34441 gtgggtggtt tgatgcagtt atagttaagt atgcagctag agttaatgga ttgactagta
    34501 tttcatttat gttacttgat gtattaactg gatttgataa gataaaagtt tgtacgtctt
    34561 ataaaatggg cgataagata ataacggatt tcccagcttc attagatgat ttagcaaaat
    34621 gtgaacctgt atatgaagaa ttagatggat ggaatgaaga tataactcaa atagataact
    34681 ttgatgacct tccagaaaat gcaaagaaat atgtagcaaa aatagaagaa ctagttggag
    34741 taagtgtaga tatggtttct gttggtccaa atagagctca aactataata agaagaaata
    34801 tatttgctta atattttatc aattaaataa tttaaaatag ttgaagaaga gtatctcaag
    34861 ataatgttta tagttattag agatgctctt ttatatttga cttacattga ttttttatta
    34921 agttaaagct acttatacat attctaactg aaattaaagt tgaatttatg tagatacata
    34981 taggtttttt aaaaagaatt ttgttgaaaa taaatggcta aagatgattt taattatatt
    35041 ctaaaaaaag taaaaaaaac tattgactaa aaattatata gacgctataa tataacttgt
    35101 gagtgaaaga aacgacgaaa aaagtcgaaa aaatgtggtc tattagctca gtcggtagag
    35161 cacctgactt ttaatcaggg tgtcccgcgt tcgagtcgcg gatagatcac caaaatatgg
    35221 ctcgttggtc aagaggttaa gacaccgccc tttcacggcg gtaacagggg ttcaattccc
    35281 ctacgagtca ccagtatggg actatagctc agctgggaga gcacctgcct tacaagcagg
    35341 gggtcacagg ttcgagccct gttagtccca ccaaattttg aattagagat aaatactaga
    35401 ttatatgctg gtgtggctca acggtagagc agctgacttg taatcagcag gttgtaggtt
    35461 cgattcctat caccagctcc acaaagtaca taaattcaaa ttaatataat tttctatgtt
    35521 acaggatact tttaagtatc tttttttaat ttcattttta ttgtattaaa aatgaaatta
    35581 aacatacaac aaaattttga attacttgca atgtgatagt tttaaatata aaatttagaa
    35641 tcattctagc ataatattat gtaatatagc aaatttaatt tatagtatca ataaatatag
    35701 actactaaga atgtaaatga tttgttagat taaggtacat tagatatgta aatagaaaaa
    35761 taaaaagtat gttttaattt atgtgatata agttttaaac agagaagcaa gtttgctctt
    35821 acctctctat ttaaaaataa aaaaatgttt tttaagggct attttattta aatctttccc
    35881 atctcaaatc atctccagta aattctaaaa tcctaaactt atctaatatt ctagagagag
    35941 ttctttgagt gtaagtattt cctatttgag atggtgttaa gttggttgaa ataatgattt
    36001 ttttaccagc aacaagtctt gtatttacaa tattgaagat ttctccacta gtaaaagaat
    36061 tattaagttc agttccaagg tcatctatta ttaataaatc acaatcaaac aagtttttat
    36121 aattatcttc acttatttga ttatttgtgt ctcttctaaa tttataatct tcaagaatat
    36181 ctaaaattct aaaagaagtt tgatagatta caacattgcc tttatctaac aattcctttg
    36241 caatacaatt gcacatatag gtctttccta gtccagtaga accataaaac agtagatttt
    36301 cactattatc cttcttaaaa tcatgaacaa aattttcgca gatacttaaa ttatttagca
    36361 tattttctct aggagatatt tcaccatcag agcctttctt aggagaaaat atattcaagt
    36421 taaaattcga aaaattttcc tgacttaaaa tcctatctaa attagacatt ttataagctt
    36481 catttacaat ttcttgtttg aggcaagaac atttttctcc attaggtaaa aatcctttat
    36541 ctttacaaga aatacattga aactttattt ccaagtaatc aagaggtatg ttactttcag
    36601 ctaaaagtct ttctttcttt acttttaaag attctatatt ctctttagtc ttctttacaa
    36661 tttcatcttt agacttagga tttaaaagta ctatcttagc taaactgaga cctattttag
    36721 aaatttcatc atctatagat tttatctcag ggattctatt atatacctca ttttttctat
    36781 gttctaataa gagttcatta tcatctcttc tcttagcata tttagcaagt atttttctta
    36841 ttttatcctc attcatttaa tcacctactt aaacttttct ttttgacttt ttttaataat
    36901 ctcatcgagc tcatcagagg tatactgagt gaatgtttcg ttgaaattat gaaacttagt
    36961 ttttttgtaa gtattgttgt tttgagtagt attaatctgc ttcttattaa tattctcctt
    37021 aactatacgt tcttcctctt tttgctttaa atcatttaaa ttttttatat ttttttcatt
    37081 ccaattttta attataccat ttatataaga tatacttggg tttgaaatat tcttagactt
    37141 agaacatgca tttattatca aatctatatc catattaaat ttatcaaacc aggtgtccat
    37201 aagttctttt tctgattttg atggttgtct tgaaaatccc aattcattga aaatcgtttt
    37261 atataaaata tatcgttcac ttcttaccaa aaagctttct tcaacatctt ttggcgtata
    37321 taaatttgaa tcataccaat ttctaataat gccttcaatg tatttaactg gctttgatgt
    37381 accagtttta tctttgacgt attcatatgc gcaaagtatc atatcggggc tcatattgta
    37441 tttattcatt atatccataa tactcatctt ttcacttggg tcgagatatc gcccaactat
    37501 tttatttata gaattaaaca tttttctaat acttggattt tcagaagtag atacaacttt
    37561 atctgtatta gattttatag atgaattatt gcttaatata ttctcaatat agaaattttt
    37621 taaatctaaa aattctattg aataattaaa attatcatat tcttcattgt catgtatttt
    37681 tataatcttc ttttcttccc agaatttcca agcagataac acatcagaca atggtatatt
    37741 taggtttttg gctattgaat tattgtcgaa cttaggatta gaagtaatat cacatgcttg
    37801 tctatatcct aaaagataaa cttttacgta taacccgtcc gccattggca taaatatatc
    37861 tataaagata ttgggaatag tgatttcacc taaatctatt tcattgcttt ctttaaaaaa
    37921 cattttatca cctcagatta aattatatca ttttttatat tatatttgtc tgtaaacaaa
    37981 taaaaggtca caagattagc atgtaaatat tttctaaaag tattcatttt gaaaaggagg
    38041 agatattttg gttataaaat tcaaccaaaa agtaagaaga acattatcag tagcgattct
    38101 atttatggta ttaataatag gttgctttat agaaaaggat gacagtttag aaacatacaa
    38161 agaaaaaact aattcaaaaa taaatcagta taatatggat gttatatttg atgatgagac
    38221 taagaggtta atgtgcaatc agaatgttga ctacataaat aatacaaaat caaacataga
    38281 taaaatatat ttccatatat atccgaatgc tttttccaag aaagattttg ctccttttga
    38341 aaaaagtgaa atgacaaggg cttatcctaa tggatttaac gaaggatata tagatataaa
    38401 gaatatatta aataataata gtaaaatgga gtataaaatt aaaggagaca aaaatgacat
    38461 attagaaata gaattaggga aagagttaaa accaaatgaa agaatgtcat tagatataaa
    38521 atataacgta aaaataccaa attcaattgg aagatttgga tatggagaaa atactataaa
    38581 tgttactaat tggtttccta tagcttgtgt aaatgatgaa aggggatgga atttaaagag
    38641 ttatgaaact attggcgacc cattttatag tgagactagc gattttcatg tgaaactatt
    38701 gattccaaat aaatataaaa ttgctcatac aggaaaactt attgatgata aacatgataa
    38761 taagaaaatg ctatatgaaa tagaagctga aaacgttaga gattttgcat ttatactaag
    38821 tagtaaattt gatgtaaaca aagtagattc aaagggagta gctatcaata catataattt
    38881 aaataaaaag ctttctaaaa aagcaactga agtggcaaaa gattcaataa atatatttag
    38941 tgaattattt ggaaagtatc cttacaaaga attttctgtt gtagcaagtg acttttttat
    39001 aggggggatg gaatacccta tgcttgttat gatagaccaa agtttatata atgaaaaaaa
    39061 tgaattttta ctagaatatg ttattgccca tgagacagca catcaatggt ggtattctgc
    39121 tgttggaaat gatgaaataa gtgaaccctg gttagatgaa gcacttactg aatattctac
    39181 aatagtttat tttgaacaaa agtatggtaa agatatggca aacaagctta taaaaactat
    39241 ggagatacag acaaaaagtt atttgagtga aaatatattt aagccagcaa atgagtataa
    39301 aaattctact gaatacagct tgaatgttta tacaaaaggc gcaatagcat ttaatgaaat
    39361 aagaaaagaa gttggagata aagtattctt tgagacttta aatgagtact ataataaata
    39421 taagcataaa aatgctaatg gaagagcttt tgtggaacta tggaatagta aaggtgtaga
    39481 tataaataag attataagtg aatgtaagta aatagtgaat gtaagtaaat tttgtgcaga
    39541 gtattaaatt ttatttaata ctctgttttt ttggtataat ataatttata aggcagaaaa
    39601 agaggtgaag gaatgctaaa gtattgttca gtagggagtg gtagtagtgg taattgccac
    39661 tatatagggt ataaaaatac taatatacta gtagatgcag gattaagtgg caagagaata
    39721 actacaggtc ttaaagacat tggtgtagat gcagataaat taaaaggaat atttataaca
    39781 catgagcatg ttgaccatat aaaaggagca ggtatattat caagaaagtt tgatatacca
    39841 gtatttgcta atataaaaac atggtgttct atgaaggaca aattaggtga catcaaagat
    39901 aaaaatatga aagtatttga aaatgataaa acctactcat taggtgatat catagtaaga
    39961 cctttttcaa tagaacatga tgcgtctgac ccagttggat ataattttta tacagagagt
    40021 gatgaaaaaa tgtctatagc tactgatata ggatgtataa ctcaaaatat aaaaaaacat
    40081 ctatataaat ctaaattagt agtattggaa tcaaattatg accctaatat gcttatgatg
    40141 ggttcatata catatgcttt aaagaaaaga gtaatgtcaa gtactggtca tttatccaat
    40201 gaagatgcag ctaatttctg cgttgaactt attaatgaag gtacagaatc tatattatta
    40261 gcacatttaa gcaaagaaaa taattttcct gagttagcgt atgagacttc aaaaggagta
    40321 ttagcatcaa atgatatagt agttggacaa gatgtaaaac tagatgtact atcacgaaat
    40381 gatgtttcta atgtttatga gatgaagtag aggtgaataa ctttgctaaa gagaaatttt
    40441 gaaaatgttg aggataataa aattttatac tctataaagc agggtatgat tttagcgata
    40501 cctgctatta tgactggttc aactgctctt gtaattttga atcttccaat aaaggcttat
    40561 caagaatatt tatcaggctt gtttaatgga gaggttacaa atattcttaa ctttataaat
    40621 gattctactt taggaattat ttcattaata atattattaa caataagcta tagttatgga
    40681 aaaatatatg ggagcaagta tacagtactt gttcctatag ttgcaatgtg ctctttttta
    40741 gtattttcac atggtaatga gcttaattct tatatagaaa tatttaaaac aaagtggtta
    40801 tttacatcta taattgtttc tatgacatct tctgtattgt ttgtaaaact tacagaatct
    40861 tttgttacaa gtgtaaagtt tcatacagaa ggggctgatg cagactttaa tatggtagtt
    40921 tcggctatag ttcctttcat aatagtagta tttttgtttt ctatttttag agtggtaatg
    40981 ataagtctaa ttggcagttc aaactttcaa gatatatttt ataatttatt ttcaaatgtt
    41041 tttaataaaa tgggaacaaa ccttatgagt gctctgttgt ttattttttt aatgcacttt
    41101 atgtggtttt ttgggattca tggtagtaat gtattagata ccgtcgcaaa aaatttattt
    41161 gaaaatagta tggcaattaa cataaattta gttaataata atcaattacc aacagaaatt
    41221 tttactaaga ccttttttga tacaatggtt ttattgggag gatgtggctc tttactatgt
    41281 ttagtaattg caatttttct tagtgaaaaa cgtataaatg taagaaagtt agctaaaatt
    41341 gcttcaatac cagctctttt taatatcaat gagatgctag ttttcgggat accaattgtt
    41401 tttaactata ttatgtttgt accttttgta attactccta taatcttaac cgttacaagt
    41461 tatgtggcta tgtctacagg tattgtacca tgtacagttt cagctgtaga atggacctca
    41521 cctatatttt taagtggata tatgtctaca ggttcaataa gaggtagttt gttgcaagta
    41581 tttaatttat gtgttggtgt tatgatttat attccgttta taaaaatgtc gcaaaataga
    41641 tatacatata tgtttaaaaa taatatagaa aatttaacaa acttattaaa aaggtatgaa
    41701 ttaacaggtg aacagcctaa tcttctaaac cataatggta aaataggttc aatttcaaaa
    41761 atgttggcat cagacttgaa gtttgctatg aaaagaggag aaatagagct tttttatcaa
    41821 ccacaagtca attataatgg aaatgttgtg ggagcagagg gattacttag atggaaacat
    41881 tcaataggag ggttcattta tccaccacta gttataattt tggctaaaga agaagggttc
    41941 ttagatgagc ttggagagta tattattaat aaagcatgta ttgatttaaa gaagagtgaa
    42001 aagctattga gaaatcctgt taagttctct gtaaatatat ctccagacca acttgatgac
    42061 cctaagttac cagagcaaat aaaaaatatc attagtagta atgatataaa tcctaatatg
    42121 ttaggtatag aaataacaga gcaaattgca ttatcaggtt cacaaataat tattgaacgt
    42181 atcaatgcga tacataactt gggaataaag ttaattatgg atgattttgg gatggggcat
    42241 agctctctta tatatcttca aaataataac tttgatatag ttaaattgga tggttcatta
    42301 gttaaagaga tacttacaaa taaaagaagt agtgaaatta taatgactat tgtaaatctt
    42361 tcaaaaaatt tagattttga tatagttgtt gagtatgttg aaaatataga ccaaagggat
    42421 aagttatatg gtttaggatg tgaaatatat caaggatatt attatagcaa agcaatacct
    42481 tttgaagagt ttgtagaata tgcaaatcaa gaaagagaga tataatttta taagagcttg
    42541 taaattctag taatgcatag atttgtaata aaattaagtt taggagaaat atatgaatat
    42601 aagtatagta gtagtaggta aaattaaaga aaaatattta aaattaggta ttgatgagtt
    42661 taagaaaagg ctttctaagt attgtaagtt agaaactata gagttggatg atgaaaaagc
    42721 tccagagaat ttgagtgtta aggaaatgga aattataaaa gacaaagaag gaaagaaaat
    42781 tttagggaag ataaagcata atagttatgt tatagccttg gctattgatg gtaagggttt
    42841 atcctctgaa gagcttgcta agactatgag tgatttagca gttagaggta atagtagtct
    42901 ctgttttata ataggaggtt ctttagggct ttctgatgag gttttaggaa gggctgatta
    42961 taggttatcc ttttctaaga tgacttttcc tcatcagatg atgagattga tattgttaga
    43021 acagatttat agggcttata ggataaataa tggagaaccg tatcataaat aaatgatacg
    43081 gtttttgtta atttgacact taataatatt aaaataacaa aaaagtattg aaagtatatt
    43141 gcatttaagg tagaattaaa taaaatatta taacattata atgtttatat ggttgttaat
    43201 aaaaatttgg aggaaaatat atgataaaaa taatgttggc ctgttcagca ggaatgtcta
    43261 caagtttatt agttactaaa atggaaagtg cagctaaaga aaatggtata gagtcgcaaa
    43321 tttgggcaat accagaatca actatacaaa atgaaataga aaaatgtgat gtattattac
    43381 taggaccaca agttagatat gtattaccta aggctcaaga aattgctaag ccatataata
    43441 taccagtaga agttataaat atgatgcact atggaacagt taatggagaa gctgttttaa
    43501 atagagcgat agaacttaac aacactaagt aatataaatt ataatgtatt tttaatatat
    43561 aaattgggag aatcaaagga tggatgaaaa gactatagaa atatctttta atataatagg
    43621 ttatgcaggt gaaggtaaag gattagcttt tgaagcaata aaagaggcta agaatggaaa
    43681 tatagaaaaa gcaaaagaat tattaaaaga gtctagggaa gttataaata aagcacatag
    43741 atatcaaaca gaattaatac aaaacgaagc tagtggaaac aggactgaaa taagtgtcat
    43801 acttatacat gcacaggacc acttaatgaa tggcatgaat tttcaacagt tagctgagga
    43861 aatcgttgat ttacatttga aattacaaga taaataaaaa ttaaagataa taaattaaga
    43921 gagttcttaa aagatacatt atgtgtagca cactaattgt tagattattg attcaatatt
    43981 tagtgagtaa tacattagga ttattttgag gactcttttt atatttagtt taaagttaat
    44041 aaatatttat tattaataat ataagattat atgttataat attataacat ataatttaag
    44101 aggtgaatca tgttaagaaa tcaaccatta tatatacaag ttgtagatat attaaagcaa
    44161 aaaatattaa ataacgaata taaaattgat gaaaatattc caacagaaag ggagttagag
    44221 caagagtttg gagtcagtaa aattactatt agaaaagcta ttgagatatt agaatcagaa
    44281 ggatatgtag agaaaaaaag tggaaaaggg acaaaagtaa taagtaatag cctttttaat
    44341 aagctatcaa aagcagagtc tttttcaagt atacttttat ctaaaggtca taagttatct
    44401 aaagagacta tttcaattga aaaaataata aatgatacaa attcacaagt atttagtata
    44461 tttggagaaa aatgtagcaa gattactaga atgtattatt tagatagtaa gccatatata
    44521 ttttttactc attatattcc ttttgatgaa aatctaactg ttgaaagttt taataatgaa
    44581 agttctttat atatgtactt atataggaga aatataaata taactagatt tgaagatgaa
    44641 ttttttatag aagaatcaga actagaggta tcaaaggctt taaatctagc taaagacata
    44701 ttattaggaa gaaaaagaaa gacctatgat gagaatgata atatagttga gctatcattt
    44761 gcaagatata atacagaagt acaaaactat gtaattaaat acgaaatata ataagaatcc
    44821 tgagtttatt gaaatgataa tatgctcagg atttttgttt agttgtaaaa ataaaaaata
    44881 ttataacatt ataatgttta caaccaaaca ttataatgtt ataatatttt taataaacaa
    44941 gtgatattat caattacata aaaattagtt tatgttatga aatcgttatt gcattataaa
    45001 taaaggataa tatcaataat acagggggat aactatggaa aaattcgcaa gttttttaga
    45061 gcgcaagatt atgcctgtag cagcgaagat aagtaatcag agacatatga gagctgtaag
    45121 agcaggtata atagcaacat taccactaac aatagtgggg tcatttttca caatattatt
    45181 aaacatacct attgatgctt atatggattt tatagctcca tttaaagcaa tattagatat
    45241 accatttaga tatactgtag gatttttatc tctatatgct acttttggga tagcatcatc
    45301 acttgctaaa agctacgatt tagatacaac aggtgttggt atgcttgcag ttatggcatt
    45361 tttagtttct acaatagttc caactcaagt agttgaacca gtaggtggag ttatagaagc
    45421 tggtagatgg atggatatgg caaaacttaa ttcgccatca ttatttggag ctatcgtaac
    45481 atcattaatt acagttgaaa tatatcgttt tatgaaggaa aagaatatta caattaagat
    45541 gccagcagga gttccagtag aggtttcaaa ttcatttact gcattattac cgacagcagt
    45601 aattttaatg ttattctggg taataagaca tattctagga tttgatataa gtgttgcact
    45661 aagcgctatg ttatcacctt taaaaggatt cttatcagga aatagtttag gtggtgggtt
    45721 actgacagta ttcctaataa ctttattctg ggtacttgga atacatggtc cagctataat
    45781 gggaccagta ataagaccta tatgggacat gtctatagca gaaaatataa ctgcattcac
    45841 agaaggagta tcagctcatg caatgccaaa catatttaca gaacaatttt tacagtggta
    45901 tgtatggata gggggagcag gggctactct tgcattagtt gtaatgttta tgttttctaa
    45961 gtcacaatat ctaaaaagtt taggtagatt aggattttta ccagggttat tcaacataaa
    46021 tgaacctata atatttggag caccaatagt tatgaatcca atactaggga taccatttat
    46081 aattggacct ataataatga caattctatc atatgttctt actattacag gggttatacc
    46141 tatgatggta gcaagattac catttgcaat gccagcacca atagcagcag taatgagtac
    46201 taactggagt atactagcag gtatcttagt attaatcaac tttgtgatat catttgctgt
    46261 ttattatcca ttcttcaaag tttttgaaaa acaacaatta caaagagaac aagaagaaat
    46321 gagtgaaaat ttataaaaat acatagatat agaggttttg tactatataa aatatagaaa
    46381 aagcttataa gttattaagg gtgaaggtag gaggaatgac ctttgccttt aattttatat
    46441 attttaaata atgtaatttg aggaggagaa agatggaaag aagattagga atatcagtat
    46501 atccagagca tagtaacatg gaagatgata aggcatatat aaaaatggct tcaaaatacg
    46561 gatttaaaag aatatttatg tgcatgcttt caattacaag acctaaggaa gaagttaaaa
    46621 aacattttaa agagattata acctatgcaa gagaattagg atttgaagta atccttgata
    46681 tagctccaaa catatttgat atattacaaa tatcttatga tgatttaaca ttttttagtg
    46741 agttaaatgc tagtggaata aggcttgact atggatatga tggaagtaaa gaagctatga
    46801 tttctttcaa tccatatgat ttaaaagtgg aactaaatat gagtaatgat gtagcatact
    46861 tggataatat aattacttac aagccaaatg taaataatat atatggttgt cacaattttt
    46921 atcctcaaag gggtacaggc ttgccatatg atttttttgt aagatgttca aatagattta
    46981 aaaagtatgg aataagaact gctgcttttg ttaattcaaa atacgcaaca atagggcctt
    47041 ggaatataaa tgatgggctg tgtacgcttg aaatgcatag aaacttagat attgctacag
    47101 caaccaagca cttattcgca acaggattga ttgatgatgt aattgtaggg aatgcatatg
    47161 caagtgaaga tgaattaaaa agaatgtctg aagtaaataa atatcaagtt gtatttaatg
    47221 tagtaactga agaaaaaact tctgaagtag aaaagacaat aatggcaaat gagcaacact
    47281 ttagaagagg ggatataacc aaccaagtag caaggtctac agaagtaaga aaaaaatatg
    47341 ctaaagaaaa tttccctaag catgataata cgaccatctt taaaagagga gatgtagtga
    47401 ttggtaatga tgactttgga aagtataaag gggaattaca gttgatactt caagattgtg
    47461 cagatgaaag aaaaaataag gtgggtagaa taaaggaaga cgaaataata cttttagact
    47521 ttattgaacc ttggagtaaa tttaaactta ttttagaggg agagtaaagt gaagatagac
    47581 atattaataa aatgtggaaa aacagtagat aaaagtataa ttgatatagc tatattagac
    47641 gataaaataa tagaagtaaa aaatcagata gatgattcta agtatcaggc aaatcaaatt
    47701 ataaacctag atggagaaaa atttattagt gctggttgga tagatataca tacgcactgc
    47761 tacgaaagac tagaccttta taaggattat ccagatgagg ttggaataaa aagcggtgtt
    47821 actactgtag tagatgcagg aacaacagga gctttagata taggtaaatt ttatgaagat
    47881 actaaaaaat ataaaacaaa tgtatatgct ttaataaata ttgctaagca gggaataaca
    47941 tctcaagatg aactttcaag tatgatgaat attgatgaat atgaacttag aagagcagtt
    48001 aaaaaataca aagattttgt tgttggaatc aaagctagaa tgagtaaaag tgttgtaatt
    48061 tcaaatgatg ttgaaccttt aaaagtagct aaaagaataa aaaatgaact taatctacct
    48121 atgatggttc actttgggag ttctcctcca acgatagagg atatatttga ctatatggaa
    48181 aaaggagata tattaaccca tatatataat ggaaagccaa atggaatact tagaggcaat
    48241 gaggttaaaa aagaaatcat agaagctaga gaaagaggta taatccttga tgtaggtcat
    48301 ggaaccgaaa gttttagtat ggatattgca atgaaatcta aggatgcagg aatttttcca
    48361 gacactataa gtacagatat atatattaaa aatagaataa atggtccagt gtataatcta
    48421 tctacaacaa tggaaaagtt tatatatatg ggctattcac ttgaagatat aatagataaa
    48481 gttactaaaa atgcagcaga tgcaataagt ttaaaaaata aaggtttaat aaaagaagga
    48541 tatgatgctg atttaactat atttgatgtg gttaatgaag aaaaagaact tcaagattct
    48601 ttgaataagt cagtaatcac aagtacgtct ataaagccaa gagcagtagt agtcaatgga
    48661 gaatatttaa atataggtaa gtctatagga gagaatgagt agaagggaga aactattgta
    48721 tgagtagtat atttgaaaaa tactctgtaa gacgtgttat aaatgcttca ggcaaaatga
    48781 ctatactagg agtatcaaca gtagataagg aagttattga agcaataggt gaagggtgta
    48841 aaaatttctt tataatagaa gaacttataa ataaaacagg tgaatatata gcaaatttat
    48901 tagggtgtga aagtgcacta atagtttcta gtgcatctgc tgggattgca cagagtgtgg
    48961 gagcaatgat agcaaaagat gatatgagtc tagtttacaa tgtaaataat cctaagaaac
    49021 aagttaaaag ggaaataata attccaaagg gacataatgt tgattatgga gttccagtag
    49081 aagtaatgat aagacttgga ggaggagaag ttgttgaggc cggttatgct aatatgtgta
    49141 acaaggaaca tataataaga gagataaatg aaaatacagc agcaattatg tatattaaat
    49201 ctcatcattg tgttcaaaag agtatgctaa gtgtattaga tgcagtacaa gttgctaaag
    49261 aatataattt acctttgata gtagatgcag cagcagaaga agatttaaat gtatattatg
    49321 aattgggagc agacctagtg atatacagtg gtgctaaggc tatagagggt cctagctcag
    49381 ggttagtaat aggtaagaaa aaatacatag ataatgtaaa gcttcaatca aagggtatag
    49441 gaagagctat gaaaatagga aaagaaaata ttgtaggtct aacatgtgca attgagagat
    49501 atataagtaa tgataaagta actttaaaag aaatggagga taagctaaat ccatttatag
    49561 acaagataaa tactataaag ggagtgagtg ctagtataac tagggattca gcaggtagag
    49621 aaatacttcg tggagaaatt gactttaatg aagatataat aaataagtca acacaacaaa
    49681 ttataaatga acttagaagt ggagagatag ctatatacac tagagactat aaggctaatg
    49741 aaggcaagat tgagattgat ataagaagtg taactggaag tgatttagat actatttata
    49801 ataggataaa aaatatagta gaaagttaga gtgtagagta agtgacatta taatgattta
    49861 ataaattaaa ttttaccaat ggaggggata aatatgaatt gtataccaca atattataaa
    49921 aatagagttt gtttaaatgt tcttgcagga agcataaaaa atgctaaaga agtatatgat
    49981 gcttgtgacg ggcatgtatt gataggaata ctatcaaaga attatagcac tgtagaagca
    50041 gctattgaag atatgaaaaa atactcaaag gaaatagata atgcaatatc tgtggggctt
    50101 ggagctggag acccaaatca gtcaaatatg gtatctcaga tttctaaaga acttcagcca
    50161 caacatataa atcaagtgtt tacaggtgtg gctacgtcta gagcattgct tggtcaaaat
    50221 gatagtataa taaatggatt agtttctcca actggtaaac caggatttgt aaaaatatcc
    50281 acaggtccat taagttcaaa agaaaaagat ggtatagttc ctatagaaac agcaatagct
    50341 atgatgaaag atatgggagg aagctcaatt aagttttttc caatgggagg gcttaaaaca
    50401 aaggatgaat atatagaagt tgcaaaagct tgtgcaaagc ataattttta tttagaacca
    50461 acagggggta tagacttaga taacttcaaa gagatagttc aaattgcact tgatgctggt
    50521 gtagaaaaag ttatacctca tgtttataca tcaataattg ataaagaaac aggtgaaacg
    50581 aaagtggaag atataggagt actttataaa ataatgaaag aattattggg atagatttat
    50641 gaatatatta gcttttggtg aaataatgat gagactttca gtacctgact acaagttctt
    50701 aacacaaacc aatgaactaa attatataac tacaggcacg ggattaaata tattatctgg
    50761 acttaaaaac tttggataca atacctatat gttgacaaag ttgcctaata ataatgtagg
    50821 taaagcatca agtgcaaata taagaaagct tggagtaaaa gatgacttta tcacgtatgg
    50881 agggaatcat ataggagtgt attttcttga aaatgggtat ggagaaagac cttctgaagt
    50941 gacttatttg aataggttaa atagttcatt ttgtgagagt aaattacaag attatgatat
    51001 tgataaatgt ttagaagaaa tggatatagt ccatatttgt ggtattgcta tgcaacttac
    51061 agattcagtt aaagagattg ctttagaact agctagaaaa tcttatgaaa aggggataaa
    51121 agtattcttt gattttaatt ttagacaatc attaaatcca gaaaggactt atgatgattt
    51181 agtaaatgat tataagaaaa tactaccata ttgtcatggg ctgtttgcta gttatagaga
    51241 tataaataag atacttaata tagatagtct aaatataaaa tctagttcag aattgattaa
    51301 aaaagagtat gatatagata ttgtagctgg aactatgaga agatttgatg atatgaataa
    51361 tagatttata aagggatata tatcaaatga tgatggttat tttgaaagta gggagtataa
    51421 gctagaaata tatgacaggg ttggtgccgg agatggatat gtagcaggaa ttatatattc
    51481 atatttggaa aatatgaaga gtgaagatat agttgagttt ggggttgcta gttcggtgtt
    51541 agctcataca acttatgggg ataattcttt agtaagcaca ggtcatgtac ttagactgat
    51601 gaataatgaa aaaatagata tattgagata atacatttgt atataataaa atttttatct
    51661 tttttagaca catttttgac ttgcttatat ttgttaaaca gagttatttt taatgattat
    51721 aaatattaaa ttaaaagtta aataataaaa tagtagaaaa tagcattaac tatagaaaaa
    51781 atggttaatg ctatttttat cgttaatatt gattaatttt atattataga aatgaacttt
    51841 tctaatgctg gatttgtatt ttttttattc catacaacta caacttcaat tctattttct
    51901 acttctttta taggtactat tgcaatatca cgttctatca ttgtatctgt aacacaaaat
    51961 gaaggtaaaa tggatattcc catgttagaa gataccaatg attcaatagt attaagatta
    52021 tttgaacgtt gtaccacatt aggtacaaac cctgcttcac gatatctatt ctgtatcatt
    52081 gacttttgtc catactcatc ccctgtttcc tttacaaaaa taaatttatc atgttgtaat
    52141 tcaattaaat cgatagaatc ctcattgtaa agtggatgac caggaggtaa aagagctacc
    52201 aaagaatatc tttctacagt tttatattca aaatctttta atggttgatt ttctggatta
    52261 ataacaaaag cgaggtccat agaattattt tttactaagt ttaaaagttc ggttatttca
    52321 ttagtacaaa tatccataga tatattaggg tatgtattag aaaattcttt taaatattgt
    52381 tgaagtttgt ttttttcata tcctattaag gttccaatgc tcaaagttcc aaaaaaacca
    52441 tgtgcaaatt gttgtgtttt tgcaatagca tcattgatat tttttattat agtacgtgcc
    52501 tcagataaaa aaacttttcc agctggtgtt agttcaacat gacgtttact tcttgtaaat
    52561 agagttacat ttatttgttc ttctaaagct tttatttgct gtgtaacagc agtttgagaa
    52621 atataaaatt tttctgctgt tttggtaaaa cttagatttt ctgctacaga tataaaatat
    52681 tctaattgtt tagtattcaa ataatcaccc ctatttaaac ttgattgttt gtaaacctta
    52741 tttatctata attaaagctt attatagtat aagaaaattt acttttaaag aatattagaa
    52801 aatatttgta taaaaatata tgttttaagt atatctttac attagatttt ataaatttat
    52861 taataagaat aacttatata ttccaatgaa aacgatattg gatttaagta agtttgtctc
    52921 ataaaatgaa agtatatcaa ttgataatac tttaaataag aagggagaaa aaatggaaga
    52981 taaattttat gcaaaaggca acggaaataa cggatatatt aaaaatcttg aagtttgttc
    53041 ctttaataac ttagatggaa cttgtggaat gtttcaaatg gctctgtaca aaagagatga
    53101 aaaatactat ttatatggat gctgttttgg aggaaataaa aaaaatggag taatgattag
    53161 cgatattaca gacccttata atccacaatt tataaaacat tttcaaatgt tagaccctaa
    53221 agagtatcct acaacaacaa ctcccaaaat tcaaatagca gatgatttaa tgatagtagc
    53281 aatgagttgt ggaagtggac caggagcact tgttgaccaa gctaaattag caaatattaa
    53341 gtgtgaagca ggaattagaa tatatagttt aaaagaagac cctttaaatc ctaagttttt
    53401 aggatattgg gattgtggct taaagcatgt aatgggtgtt catagattca tgtacaatgg
    53461 tggaagatat gtacatttat caagtgattg tgttggcttt gaaggtctga tttatagggt
    53521 catagatata ataaatccta ctaatccagt ggaaataggt aaatggtgga gaccagacca
    53581 atatgcaaat ggatatccaa atagaacttt tgatgcagga gcacctcatt gcccagaatt
    53641 tatggataaa ggatggcttc atggacctcc atttgtaaga gacggaaaag catattgtgg
    53701 ttatggagga gctggtttag ttgtattaga tgttgaagat ttaacaagac caagatgctt
    53761 aggtgaattg ccatttatgc ctgcattttc tagtagactt gcaggtgcaa gaactcatac
    53821 agcattacca ttgccaggaa gagatttagt cgttgttcaa aatgagggag aaagattcca
    53881 gttctttaaa ccagataaca ttacagatgt tcaagctatg aataatatac atatggttga
    53941 tgttagtgac ccaacaaaac caacattaat tgctcaattt ccatatcctg aagttccaaa
    54001 agatttccct tatcctaact ttaatgttgc gggattagga aaaccagggc catttggccc
    54061 acataatctt catgaaccaa tggataataa gccatggtta gagcaaagag gagatagagt
    54121 atattgctgt tatttccatg cagggctaag ggtttatgat gtatcagacc catattatat
    54181 caaagagcta gcatatttta taccaccaaa tccaaataaa acaccagaag aatcttattt
    54241 cccaggattc ccaggaccac gcttggcagt aacagaagat cttatcgttg atgatagagg
    54301 ctacatcatc atagatgctt tagatgatgg attctatata ttaaaaatga aagaagatta
    54361 aataaatact aaagagggag agttgaaaat gaaaatacta ggtatttctt ttggaacaaa
    54421 aaatggaaat aatgacacaa tgtgtaaagt tgcacttaaa ggtgccaaag aagcaggggc
    54481 tgaagtagaa tttattcaaa tgtcatctat taacattaaa cattgtactg gatgttgtgc
    54541 gtgtgttaaa actttattat ctgggaaagg tagtatgtgt gtattaaaag atgattttga
    54601 atggttgctt gacaaaatga aagatgcaga tggtatagta gtttcagacc ctatttttga
    54661 agaaggggca tcaggattat tccatactat aatggaccgt tttggaccca gagcagatag
    54721 aggaaataac attataggaa caaaagttgc agaagctata ggaggaaaaa ttccagattc
    54781 aagaatgctt aatgataaag taatatcatt tatgggaata ggaggttctg actggggtac
    54841 aagagttcaa tgtgaacatg ctatgctagc tctcatacca atgtggaaag ttatagataa
    54901 tgcttggttc ccatgggcaa aggaattagt aatggatgat acaaggcttg ttcaagttca
    54961 tcaaattggt ttaaatatag ttgaagcagc aaaagatttt gaaaaagctt catatcaagg
    55021 agaagaaggt gtatgtccac attgccacaa tagattattc taccttgagc caggtactaa
    55081 aaaggcaatt tgtgctctat gtggtatagt tggagagtta gatactataa gtggaaaaac
    55141 tatatttagt tttccagaag agcaattagg tcatgctcat gatactttac caggtaagtt
    55201 tatacatggt gatgatatta agaaaatgga aggtcattat gcagaagttc gtaaaacaca
    55261 agaatttaaa gaaagaaaaa catcttataa ttttatagag ccattaacta aagaaaagca
    55321 agctggagtt tcattaggat aataattaaa atactaagac tcttttttaa ggtatgttag
    55381 atgttatagg aggcttattg tatgaatgaa aagtcaagtt ttaaacaaat tgaaattatt
    55441 ttagcagtta tttttttggt acttacatat tacaaggtta gattaactcc agcatttatt
    55501 gttatattta taccactata tttaagttat tttgcagtat ctatgattgt aaatcaaata
    55561 gcaattaagc tattggagat aaggcaagct agggaagcta ctaattacaa tataggaaat
    55621 ggatagaaag aaaaaagaaa tattaacact gtctatgggg attgctttac tacctccttt
    55681 atgggcagta ttagcacctt atataggaat aaaaactgga gctgtagcac taatctgtgc
    55741 aggattatat gtaactaatg gtaataaaca aaaagatgga ttaaaaataa tgtttggttt
    55801 ttggtgtgga gatttatggg cagtgttagc cattttaatt atgggatata tgaatttcaa
    55861 tcaaaaccta gaattatttt taacacttag tatattaggt ttttttgcag ttgtaattgc
    55921 atccttattt gaaaaaataa ttttcctacc atcatggctt tgtggttggg ctataggtct
    55981 aactattatg acaggtgaaa atattattaa tttacaagat atttgtattc aaattgctgt
    56041 agctatggca gtaggagtat ggtacgtggg tgcaggtgta gatttgttta caatatatat
    56101 tttgagtaaa gataggaaaa agggaaattg aaagcatgtg aatatacttt gattatagga
    56161 gaatggctat gggttttgaa tttaaaataa tgagaagttt aatatatgta ggacttgcca
    56221 aggaagaata tagacctaag ctaatggact ggttatatcg tcaccatatt ccagatagta
    56281 ttagcacttt tggaccatat tgtactaaat atgcctttta tcaagcatat cctacaccaa
    56341 atgaaggtga gcgttttggt gcacgtaaga tgcaactaac agaacattat tggcttgtag
    56401 atgaacatat gcctgagatg gcaaatagaa ttatgacaga atatatgcct atggatgttc
    56461 tacgttggca agggtgtata ccagatgtag aaaataaaag ggttcatgaa aatgcagaaa
    56521 gtggagatgc aggacgtgca gtaggtggag ataatggatg tccaccattt atatttgcct
    56581 ttgttccaat aaactgggaa gaagacttta gaggaaaagg acgtactgta caagatggac
    56641 caaactatcg ttggcaattt atgattaagt atccagatgg tatctctaaa gaagaaggag
    56701 aaaaatggtt ctatgatgag gtagtgccat actttacaaa ctgttgctat gttaatcgtt
    56761 ttgtcagtag taaaataatg attaattatg gagcaactgc ttttgaccgt gtatcagaac
    56821 tatggtttga aggggaagaa gaatggtata aagctgtggt tgaagagaca aagtcgttta
    56881 ttaaaaaacc agaatgggca caagaagagg agttcccata tttaaaacca caattcaata
    56941 tcgcatcagt attcttaggt gatatagcaa ctatggatgc atactcacag tatcgtggat
    57001 atataccaat gagataaaaa ttatagtttg tgataagtag aggaggcatt tactcttact
    57061 ggtagaagaa gacttattgt atccttaaaa gtaggtgaat atatagtttt atatttaaat
    57121 ttatataaaa ctaagactaa taaatatgaa acaatagaat tagaataatt tgataatatt
    57181 tgttaaaaga gcatttagaa tgtgtttaaa gattaagata ttccttttat ggtagcagat
    57241 agttgaaata ataaaactgt tatcatgaaa ggaatatttt tatgtatagg aaatttttaa
    57301 aaataaaagt taaatatgtt taagaacatc ttaaagtgaa agaatcattg ttagaaatat
    57361 gtagacaata tagtctttat aacgtatcat attaacaagt ttacacttgg attatgaaat
    57421 aaaaagagta attccaaagg ttgtaaatac tatgctgact ttgatacttc aaagtttagg
    57481 tgattttgta attgttaata gaaatacgtt taatgagcat aaagaattta tctaataata
    57541 ggagaatata atgtatacaa ttggacaagt atctaaattt ttaggaatat ctagagatac
    57601 actaaaattt tatgaggaca aaggattagt aaatcctaag aaaaatgatg aaaatggata
    57661 tagaatatat aatcaggttg atatctatga tattgcaaca ataaactttt atagagaaat
    57721 tgatatagaa attaaaaaga ttcaagaaat aagaaaaagt aagagtataa acaatttaga
    57781 actcttacta gaagagaagg aacaaatcat actaaaagag atagaatata aaaagcttct
    57841 cttaaaaaag gttcagttag tcaaagaaga ttgtgacaaa ataaaaacat attttggaaa
    57901 atacacaata aaagaaatga agcctttgga agtaaaaggt aaaataaaac atgttactaa
    57961 ctatgaggaa tatgaagcct taaaagagaa tacaaacaac ttaaaaaaag cagttacatt
    58021 aactgattta agaagagtaa taaattttaa tgaagaaggt ataatagaag aaaaatttat
    58081 aattgtaagg gaagtagatg actacaacaa aatcatagaa ggtgaagttc tatctcatcc
    58141 caattgtatt tatactatta ttgaagatgg cagatggtca actggaggag aaaatattga
    58201 taataaagtg gaagacagat taagagaaat agcaatagaa aatgaatgtg aattacttgg
    58261 aatagtctat gttaatatat tgcttactac ttatgaagaa gggattgagc gtatattttt
    58321 agagatttat gcacctataa aataatataa aagcattgac ctgggactta ctcataggtt
    58381 taaactcatg atataagcag ttataactta tatagcaatt aaatattaat ttgaaatgag
    58441 gattacttat gagaacagag aaatcattaa aaaggcctat agccagtttt attattctaa
    58501 caaatataat ttttttacca ctttttttat tggtagtagt gactataatg ttaaaacttc
    58561 caacagtgat atctgacatt gcgttgtgta tatcagcgtg gtcatccact tttgcattta
    58621 tgatattatt taaaaaaatt tatccaggta agaaattttt agtacacgta aaagatagat
    58681 ttagaaataa acttaaattt tcaacagtat ctataataat tagtatacaa gtcctaatag
    58741 ctgtagtagt tatatttata ttagcatcta aaaatcatgg aataatgcct aactttacag
    58801 tttcatcatg gggagcattt atatatttat ttttaaaaaa tttatttgca ggatcattag
    58861 gtgaggaatt aggttggaga ggatttgttc aaaatcatct tcagaaaaga cattctcctt
    58921 taaaagcatc cataatagta ggtttttggt gggggatgtg gcatttacca atatggttta
    58981 caacaggatt caaaggactt gatttaataa aatatattat attttttatg attgcaatta
    59041 tatcaatcag tattataatg acaacttttt ataatctaaa taaaactttg ataataccta
    59101 ttgtaataca tcaactattt aattttttaa tagggataat taatggagat ttaatagagc
    59161 ttataaatta ctatgcaatg ttatatttaa tatttgcaat tgcattaata gttataaatc
    59221 caggaaatgt attatataaa aatattagag agtaatatat atctttatgg taagatataa
    59281 agatattatt agaagaacat ccaaggagga ataaacatgg ttttactgat tgttgatgca
    59341 caaaagttaa taacaaacga gaggttatat aaatttaatg aatttgtagt aaatgtagaa
    59401 aatttaattg atacagctag aaaaaataat atagaagtaa tatatgtacg tcatgatgat
    59461 ggagttgaaa atgaattgac taaaggtaaa aatggctttg aaatatacga aaaatttaaa
    59521 ccatgtaatg gagaaaaaat atttgataaa aaagtaaata gtgcttttaa agaaactggt
    59581 ctattagaat acctaacaaa taatggtgaa aaagatatta ttattgctgg tctccaaaca
    59641 gattattgta ttgatgccac tataaaatgt ggatttgaac atggatttaa tataatagtt
    59701 ccagcttatt caaatacaac tgttgacaat ttttttatgt cagcagaaca atcatataaa
    59761 tactacaatg aatttatatg gaatgaaaga tatgcgaagt gtatttcatt agaggaaaca
    59821 cttagaaaaa tgaaataaaa atagagtatg aattgtatat gtacataagt tgatttcata
    59881 aaaaaggatg ttaaattcca agttaaaaat tagattaaat atattatatt atagataagg
    59941 cacatcaatt ttattttaaa ttaatgtgct ttttaattgt agtaatttat ttataatcaa
    60001 tatgattttt attatttaaa tcaatttttc ttgagtttct ttgtctaaag aaactttttt
    60061 tatttataaa atctacagtt atattatagt atctatttta gagaagttta atttttgtat
    60121 caatctaaaa caaaagttat acttaaatga aattgaaaac gataaaattc tcaaataaaa
    60181 taagtattgt ggaggtgatg aaagaatcca atgataatat gaagactaat cttaataaga
    60241 aagtaggggt taatggtagt ggacaaagaa attgtatcaa ttctccagat tttattaaat
    60301 agcacattga ttacaactaa tggattacaa gaggagtcta attcatcaaa gagacaaatt
    60361 acttatagaa taaataaaat caatgacatg cttaaagtaa aaaatgtacc acttatatgt
    60421 ttaagagctg ataaagatat tatcgttaga aaagaaacaa aaaaagcaat taaagaaatt
    60481 ttagaaaaaa actattctaa gaatacttat tattttagta agacagaaag acttttatat
    60541 atgtatttaa tgctttttat taacatggat gatatttcaa ttaatcattt tataaattct
    60601 ataaaagtaa gtaggtctac tgtaaatctt gatttcaagg atttgatacc agagcttgaa
    60661 aagaagaaca ttaaagttaa gaacaatcgt ataaatggat attacatagt tggcaatgag
    60721 atggaaatta ggagagtgct tattaaaaat attatagaaa ctttgtctaa tgagaaaagt
    60781 tcaagagtat ttgatatttt tattaaagaa tataatttag atagttttga agaggctaaa
    60841 aaaattatat taaagctaat aaaaaaatat gaaattactt ttgttgaaga tagattagta
    60901 gaatttatat atatatttat ctttctaaag gcaaggatgt attcaactag aatagttact
    60961 caggaaaatg tagatattcc taatatttca gttatgagtt caatgaagga gtataaattt
    61021 gctaaggaat tattagaagt atatgggagt gaagataaga taaagcctca caatataatg
    61081 tatattagtg cttggatttt aggtatatca gtaggaaatt atgaagaaga tacagaagat
    61141 aaggatgtaa tttcaaaaat tgtaaataaa atgatgacaa agtttggata tttaagcgga
    61201 gtttactata taagccaaga aaaaatattt aaacaattat attctcattt tagacctgcc
    61261 tactatagac ttttatttaa attacctatt tataatccga tttgtgaaaa agtaaaagaa
    61321 gaatataaat tagtttatag aattgtaagt aatgcaatga aagagttttg cggattattt
    61381 ggagaagaaa ttcaggaaga agagttagca tatcttacaa tgcactttgc aacacttttt
    61441 tcaaataaaa aagaatttga tggttttaga aaaagaatag cattaatagt ttgttcaaat
    61501 ggcgttggta gttcagctat tttacatgca gaacttacaa acttatttcc tgacttacac
    61561 tttataccaa tgtcggaatc agttggatta gaaaatataa gtgaacctgt agatattgta
    61621 tttactacta attataacgc agagggttta aaaacagatg taccagtaat taaagtaagc
    61681 cctgttatga ccacaaaaga aaaatataag attacacgtg aagtgtatat tcaattaggt
    61741 gatatttttt taagacaacc taaaatagat gaggttatgg atataataaa aaaatatgca
    61801 aatgtaacct ctgaaagtat tctttcaagt gagctattag cttattttac acagatagag
    61861 aattttacat ctaaggaagg agaggggcct atgctaagtg agattactaa tgaaacacta
    61921 gtaaaaataa aggtaaaagc taagaattgg gaggaagcta ttagacagtc agcttctgta
    61981 ttagttgaaa ataacaaagt aactgaaggg tatgtagatg ctatgattaa ctcagcaaaa
    62041 gcatctggac catacatagt aattacaaaa catgttgcac ttccacatgc tagaccagaa
    62101 gctgggtcaa aagagattgc aataggcatt gctactttag atagtcctat tgagtttggg
    62161 aataacgaaa acgaccctgt aaaatatgta ttttgtctaa gtgcaataga caataatagc
    62221 catttacgtg ctatgtctga gctagttgaa ctattggaag aagatgagtt ttttaaggtt
    62281 ttagatagtg ctaaagaagc taaagaaatt attgatttta taaaaacaca cgaattataa
    62341 aggaggattt aattatgtat aaaattttaa tcgcatgtag agcaggagtt ggctcaagtt
    62401 taatgttaaa aataaagact caacaaatta ttaaggaaaa taactttcca attgaagtag
    62461 aacatggttc attggattca ctaaatggat tcaatggaga tgctgtagaa acattaattg
    62521 atgttgctga agaattaaaa aataaaaatt tgaagtttga tataattggg attcacaata
    62581 ttgttgaccg taatgaaata aagacagcac tagaaaaatt ccttgcttca aaagagggat
    62641 aaatttaaga tgataaggag gaatatttat ggagtttatt attagtttgc tgagtacacc
    62701 tgctgttttg cttggcgtag ttgcatgtat aggattgatt cttcaaaaga aatcagctgt
    62761 tgaagtattt acaggctctt caaaaacact tatcggtttt ttgatatttg gtattggtgc
    62821 ctctgctatg acaggagcac ttcaaaattt caataaactg ttccaacatg gctttggaat
    62881 tactggtgtt atagcttcac cagaagcagc tacagcactt gcacaaggta catatggttt
    62941 tgcagtttca tgtacattaa ttcttggatt catattaaat ttagtatttg cacgtattac
    63001 aaagtttaag aatattttct ttacaacagg tcatagtttg ttcttttctt gtgtattagt
    63061 tttaattatg aaagcacatg gatttgataa tacaatcact attttaatag gtggaactat
    63121 cttagggttt atgtctgctg cactacctca attctgtcaa ccatttatgc gtgaattaac
    63181 aggtggcgac gaacaagcta ttggtcactt taacatgatt ggttatggtt tatcaggtta
    63241 tattgggcgt ttattttcaa aacataaaga tgatacaaca gagactatag aatttcctaa
    63301 gtggttatca atattcagag acttcttaat gggtctatcc atagttatgt taatcttatt
    63361 ttatattgca acattaaaag ctggtcaagc atttactcaa gaaattgctg gttcaacaca
    63421 ttggttggtt tttccgctta tagaggcatt tacatttgtg gcaggtatgt caatattgat
    63481 gtcaggagtt cgtatgttct tagctgaaat aactgcagca tttgtaatca tttcagaaaa
    63541 gtatattcca ggttcacgtc ctgcgctaga tgttccaacc gtatttccat atgcaccaaa
    63601 tgcagttatt attgggttta tatcagcata tgctgctggt ctattagcaa ttgctattat
    63661 ggcatcattt cctagtatat ttcctgtagt aattattcca gctgctcaca tatgtttctt
    63721 ctcaggaggt acagctgcaa tatttggcaa tacttcagga ggatggagag gggctattgc
    63781 tgggtcattt gttgtggggt tattacttgc atttttacca gttatactat atccagttta
    63841 tgggactctt ggtatagaag gagcaacttt ccctaatatt gattacaacg tagttggttc
    63901 gatattacat aatattttac aatttataaa acctatttag actaaattat ataaaagtat
    63961 agtcatagtt actatgattt caaggaggat acatcttatg gaaaatacat tatctgaatt
    64021 tttagataat atatctaatg aattgtcaga atttattgcc tcaatagatg agacatctat
    64081 aaataaagca tgcgaattaa ttcttgaagc tgaaaaaaat cacggacgtg tacatgtgac
    64141 tggtattggc aaaccaggac atgtatcggg atatatttca tcattgcttt catctactgg
    64201 aacgtcagca tatatattgc atggtacaga agcagtacat gggtcaagtg gtcaagtagt
    64261 tgaaggagat gttgttattg caatatcaaa tagtggtgaa actcaagagt taaaagcaac
    64321 tcttaagact ctaaaagtaa atggagcaaa gataattggt gtaagtggta atgagagttc
    64381 atttttaaaa aatatttctg atatattcct gtttgcaggt gttaagcagg aaggagattg
    64441 tctcaataaa gctcctcgtg cttcaatttt agctgaaaca ataatattac aatcattaag
    64501 tgttgtatta caatatgcta aaggtcttaa tacacaggag tatttgaagt ggcatccagc
    64561 aggaagtctt ggaaagtcta ttagagaggg aatataattt tatgaaatat aaagggatta
    64621 tatgtgcaat gataacacca tttgatgaaa atcaaaatat taattcacaa gcaacatgtc
    64681 aattaattga ttatttgatt gaaaagggaa tttatggatt atttattttg ggcactaatg
    64741 gtgaatgtca tgtattaaca gatgatgaaa aagttgaatt tgcaaagatt gttattaatc
    64801 atacaaataa tcgtgtacct gtttttgttg gaacaggggg taattcaact cgtgaagttg
    64861 tagatttatc taagagaatg gaagcgattg gagcaagtgc attatctatt attacaccat
    64921 actttgttac accaacacaa caggaattaa tcttacatta caaaactatt gctaattcag
    64981 ttaatttacc tattataatg tataatatgc caggtaagac tggaattaat attgaaccag
    65041 agtcagtacg tgaattatca aagataaaga atattgttgg aattaaggat agtagtggta
    65101 aacttgacaa catgaaagct tatattgaag caacgaagaa tgagaatttt agtgtgtttt
    65161 caggctcaga ttcacttatt ctggatagtc taaaagctgg tagccaagga gctgttgcag
    65221 caacaacaaa cttcctcaca gagattggta tctctatcta tgaaaacttt atgaaagggg
    65281 atttggaaga agctcaaaaa tctcaaagta gtatagaaga acttcgacgt atactgaaac
    65341 ttggaacaat tccatctgtt atgaagaaaa ctattgtctt gaatggaatt aatgttggaa
    65401 ctgcacgact tcctgtaact gaaccaactg gggaagtact tgaagaaatt aaacgagtag
    65461 ttgaaaatta taggacaata cttaataaaa gtagtgagaa tagataatat aaatagggaa
    65521 attacactgg tatataatta atatatatat tataaaacat gactaaaatt aatgaataag
    65581 taaagtggtt tgatgagtat agaggtgata atatgaaaaa gatgaatata accaaaagaa
    65641 tgtcagaatg tggggctctt gcaattgtaa gagaagaaaa tcttaaccgt gcatgtgaaa
    65701 ttgctgaagg atgtataaaa ggtggtataa cagttataga aatgagttat accctaaata
    65761 atgctggaga aataattcaa gggcttaata aaaagtatgg tgaaatactt tgtgtgggag
    65821 caggaacagt tttggatagt gaaacagctc gtcatgctat attacatgga gcccaattta
    65881 ttattgcacc aaattataat gaaggtgtag cgaagatttg caatagatat cagattcctt
    65941 atgctccagg atgtacatca ttgactgagg cagtagacgc actaagttta ggagcaactt
    66001 ttataaaagc tttcccaata tcagatttct atggacctaa actagtaaag gtatttaaga
    66061 caccaatacc tgatatgcca gttttagcaa gtggagggct taatattgaa aacctacata
    66121 tatggttaga aaatggagtt gatgtatgtg gatttggtgg tttattaact aaaggttctg
    66181 ttgaagatat tgctaaaaat gctaacaaga ttcgtgaaat cattaaaaat gtaagaaaaa
    66241 tttaacatag ggctgtatca tagtatctta taattactat gatacagcct ttttaatatt
    66301 attcaaattt aaaacagttt acatttctat tatattacat ataatttcag taattttatc
    66361 ttaatttttc tatctggaaa aaaatgattt aaaacttaag ttatgatata atttcattaa
    66421 aagaaggcga tttttagtaa atatgagaaa ataatgtgtt tttaggggaa aagttaaata
    66481 cagatttaag tgaacaagaa tcgataatta agatattaaa tggtgaatag ggcatcaaaa
    66541 aaattactaa attaaaagtt ttagagggag aatcttagca agtgtgtgtg aaaattcaat
    66601 ttcaaacaaa aataattgta aatgaaatca atttatacac gttgaagatt cacttttatc
    66661 attatattaa aaagcagagt gaattaaaag tgagtctaaa gataggagga taattaaaac
    66721 aatgaaagaa gacattaggt tacatcgaat gtgtaaaaaa gaacaagcaa caaagaatga
    66781 agttatatca gaggatattc taaaatctat gagtattggg gtcagtaaac attttttaga
    66841 tgattcattt acaataatat ggggaaatac atcattctat caattattag gatatacaga
    66901 agaagaattt ttagttaaat tttctagttt aaaagattat tatgaacaga atctttatca
    66961 ttttgaatct atgaaaaatc attttttaga ggcttatcat catggcgaaa agagtataga
    67021 atatcaaatt tgcatttcag caaataatgg agaccctgtt tgggtaataa tgactggtat
    67081 attaattgaa tcacaagata atgagaaatc aatggtctat atattgtatt caaaaattaa
    67141 tgagttaaaa ataaagcaag aagaactatc tgatatggaa aaagagagta cagagaattt
    67201 taaatggatg atgtcagagt atgctgggaa tgtttatatt agcgatatgg atacatatga
    67261 attactatac ttaaataagc actcatgcaa tactttacag gcatcagcaa atgaacttat
    67321 tggaagaaaa tgttatgagg taattcaagg tagaacttcg ccatgtccgt tttgtacaaa
    67381 ttcgtattta aaaaaagatg acatatatga atgggaattt tataataaaa aactgaaacg
    67441 aacatttata attaaagacc gtatgttgaa ttggcaaggg cgaagagttc gtattgaatt
    67501 atcttatgat atgtatagtg ctgaatataa attagcaaaa aaagatcagg aaagagaatc
    67561 aattttgaaa actattccag caggaatggt tagattagat gcaagagatt atcttactat
    67621 actttggtgt aatgatatat ttttgaatat gattgaatat acaaaggaac agtttgcaga
    67681 ggaacttaat aatcaatgtg gatatttatt ttctgaagat tataaacgta gtaagaaact
    67741 ggcacaagat ttgaaggagt ctggggataa tgttgtattt gaagcaaaaa tatatacacg
    67801 ttcaagggaa gaacgtatat ggactgttac attgtgttat atcagtgggg aagacagttg
    67861 ggatggaata ccatcttttt atagtatagg tattgacata acaaaggaaa gaaaacagat
    67921 tgagaaatta cagcatatat cagagaaaga tgcattaaca ggaatttata atcgtgctga
    67981 gactgaaagg cagattaaga aatattttga aaaaaattta aatgttatgg gtgcactgtt
    68041 tatgattgat acagataatt ttaaacagat taatgataca gaagggcata tgattggaga
    68101 tatagtactt actgagatgg catcaggtat gaaaaaaata atgcgtgaca gtgatgttgt
    68161 tggaagaata ggtggagatg agtttacaat ttttatgaag aatatttcct cagttaaaga
    68221 tgcagaaaag aaagcagaag aactattgta tatgttccgt catctatttc agaaagaaaa
    68281 aagttttcta aaagtaactt gtagcatagg aatagctata tatccaaagg atggtactac
    68341 attcaaggaa atatatgcaa gagctgataa agccctgtat caagcaaaaa atatgggaaa
    68401 aaataattat gtaatctaca accaagatta ttttaaagaa ttagaagaat ttgattgttc
    68461 ttctcttggt acagttattg attcagaaaa acaatacgct gaatatcctg ataatcttac
    68521 acgttatata ttccgtatgc tttatcagac tgatgatata gaccaggcga ttaatatgat
    68581 acttgaggtt gttgggaaac agtttgatgt aagtcgtgtt tatatttttg aaaatacaga
    68641 agatggtaga tatacttcta atacttatga atggtgtaat gaaggaatct cttctcaaat
    68701 ggcttatttg caaaacagca attatcaaga ctatgaggat tatgaaaagc tttttgagga
    68761 tgaattagtt ttttattgtc gagatatcca tacattaagt cctaaactag aagaattgtt
    68821 ttctagtcaa ggtatccact ctacacttca atgtgcatat aaagaagata gagttttcag
    68881 tggatttgtt ggatttgatg aatgcacagg actacgtcta tggtcacaag aggaaataag
    68941 tacattatca ttgatttctc aaattatatc aatgttttta caacgtaaaa aaaataataa
    69001 acttaatcaa cagattcaac aataccagaa tattttagat aatattgacc agtgtatttg
    69061 tgtgattaat aaagaaaata attttttatt atatgtaaat aaaaaattta aaaatacata
    69121 ttctaaattc caaattggac aaccttatta tcttgattct atagatattc ataagatatc
    69181 cattatatgg aataaaaaag aagcatacct atgtactagc acgatttaat ggtaaagtaa
    69241 agtttatatt ggacttaaat taaatttcgt tttaaataag atattatttt agttgcttac
    69301 tgaaaagact agaatctata ttaatgaatt atactaattt tataccacta ttaaaatatt
    69361 gtttgctaaa aataaaaaat agccttaaaa cagttgatta taaaaaatta actgctttaa
    69421 ggctattttt atctttgtga cgaaatagac acttgccatt tagaatgtga caatatagta
    69481 gttgtttaga aaaggcacaa agcattatac tttaactaaa gtaaaatgta ttttgctttt
    69541 agaataaaaa caattaggag ggagctataa ttgaaaaaga taatatatgt attatttaca
    69601 attgaactat taatagtatc tttattaggt ctaaatatag ttaaagataa tgaaattaat
    69661 aatattttat ataacgatac aacaagtata tctgtaatgt ttaaagatta taaaaagtta
    69721 aataaaaatt atagtaagtg gattaaagat attgcagatg aaaataatgt ggctatatca
    69781 aagtatgttt ttaataataa tgaaaaatta tctatatata cgactgatac aagtttgaat
    69841 aacaatataa agttaaaatc aggaaaattt cctaatacta attcaaatga atttattagt
    69901 aatataaaca ctaataatag taatcaagta ggtaaattct caactatgag taaagacata
    69961 tcaattttaa ttagggattt tggtaaaata agtgaagttg gagaatcagg aatcttatat
    70021 atttcaactc aaaatgaaga tacaataaat aaaatattaa gagaactaaa tgtagacaga
    70081 agtatttttg tagcaagatt acatgataga tatgttaact caaatttata cttaaatcct
    70141 tcaataatta gagatttaat actaattatc ctttgtttct tggctacaat aatacattat
    70201 tcaattagtg tgagtagaga ggcttctatt ttaagattaa atggatactc taagctagat
    70261 ataattgcta gagttataaa aaatatactt agaattatga ttttatcatc tgtcacagca
    70321 ttattaatat atataatcta tattttaaaa ttaaatatag gtgttagagc atgcttatac
    70381 tttgctatgt tttcgattat atgtattttg ataaatattt tgatatctat attaatagtt
    70441 ggttttaata gtagaagttc taaatatgtg ctatctctaa atggaaaaaa atcttatggt
    70501 tttgtaaata taatgcattt cacattgaaa attgtatttg tatcatttct tatattatcc
    70561 ataaataact gtattttaaa ttataatatg ctacaaactc agttaggaaa tctatctgag
    70621 tgggataaag caaaaaatgt ttataatctt actcttaaag atacaggcga acaatcttta
    70681 ggaaaggaag aagtaattaa aaatgccaag attaaaggtt tctataaaaa tttggtagaa
    70741 gaaaaagatg ctttcttaat agattcaact aattttgaaa aacttgaaga tggaagttac
    70801 atgtatgaaa taaattcaaa gggtaaaaac ccagaagtaa gtcaatatgg taaaaatata
    70861 aaaataaata aaaactatct taaagttaac cctatttact caaatggcaa agaaatattt
    70921 aatctaatcg attatgacga taagactata aatttattag ttcctaaaaa gttacaagta
    70981 catgaaaatg agattaaaaa agagtttaga gaattatttt attttgaaaa aatagaagta
    71041 gaaaatatgt acaataaaga gttaaataga gagttaaata aaacaaagaa gtccgattta
    71101 aatgttaata taatatatgt agataataat caaagctatt ttacttataa ctcccttgtt
    71161 atggatgaca ataggaatct cattgatgac ccaatagcaa tagttgacat agataatatt
    71221 gatgattctt tttatctttc ttatatatct agatgtgtat attttaattc taagaaatta
    71281 gatgcccttg cagatataag tagcattatt gaagctcaag gtgtagaagc gcatatccaa
    71341 agtttacatg ctgtttataa tgagtatggt ttagaaataa ataaactaga aaaatatttg
    71401 aatagtgaaa tttttactat aataataata gcaatatcaa acttaatgat tacctacaat
    71461 atagtagcta gctactatga aagaaataaa tataagttat atattaaaaa aatatttggt
    71521 tactcaacta cagctagaag tatgttgtta atagtctcat taatattaac aaacattatt
    71581 ccaataggca ttatatcaac aagagtagat ttaagtaata atattatctt atttggactt
    71641 ctaatattag ttatagaagt aattgtatct attgtattag acaagattat ctcaaatagt
    71701 tcatttaata aaattataaa aggagaacat taaaaagatg atttcattaa taaatatatc
    71761 taaaaaattt aaagataaat ctataataga caaatttaac ctagaaattt ctaagggcga
    71821 gtttgtagcg ataacaggaa atagtggttc aggtaagaca actttactta atataatggg
    71881 attattagaa aaacctaatt ctggtgatat tataataaat aatataaaaa atccaaatag
    71941 taaacagata agaattttac aaagggattt ttatggctat ctatttcaaa attatgcttt
    72001 aattgagaat gaaaatgttg aaaataactt gaaaattgct ttaaagtatc aaaaaaatat
    72061 caatcataat gaaaaaataa attacgcact tgagtctgta ggtcttagaa aatacaataa
    72121 aaaaaagata tacgaattga gtggaggaga acaacaaagg gtagcattag caagagtaat
    72181 attaaaagaa tgtgaagtaa tatttgcaga tgaacctaca ggtaatttag ataaaaaaaa
    72241 tagagacata gtatttgata tacttaaaag tctaaataag aatggtaaaa ctatcgtatt
    72301 tgtaactcat gatttagatt tatcggagca ggcagataga gttataaaat tgtaataata
    72361 taaactataa taaatataag aaagagatta cagctaaatt aagtttaata ataatacgaa
    72421 tagaatttaa acattaagta aattagtagt ttgtaaatat aagtataata tgatatcata
    72481 aaggctcaaa agaaatttta tataataaga aatctaaaaa taacaaatgg aggacatata
    72541 aagatggaaa atttaccaaa ttgcccgaaa tgtaattcag aatatactta tgaagatgga
    72601 actcttttag tttgtccaga atgctctcat gagtgggcat taggattggg agctgaagaa
    72661 gattcaaata ttatcaaaga tgtaaatgga aatgtattaa atgatggaga ttctgttaca
    72721 gtaattagag accttaaagt aaaaggaagt tcatcatcta taaagattgg aacaaaagtg
    72781 aaaaatatcc gtttaatcca tgactcatca gatggacatg atatagaatg taagatagat
    72841 ggttttggag ctatgaagct aaagtcttct gttgttaaaa aagcgtaata ttataaataa
    72901 gctgtagtgt ttattgtttt tcagtacagt ataaaaccat aagaatctat taagtaataa
    72961 aagtttgttt atttatcttt atattttctt atggttttat taaatataaa ttttattgaa
    73021 caccaaaata ttctctcctc tacaaataag ccaatcttat ttaaactaat atactttgta
    73081 ctatttatac tgaaattgta aatttaaatg ctatgttatt taggtttgta tagttaattt
    73141 atatatttct gattttataa attgtattta ttattatatc ttgattctaa aatcattaaa
    73201 aatttcaact tctataaaga tttacaattt atcaaataca ttatgagtat ataaacaatt
    73261 tattattaca gtgctacatt taaaataagt cttagtataa aggtattgtt acaagtataa
    73321 agaaacttat gtatgctatt ggaataaata ataccttaaa ttataattaa gaataagaac
    73381 tgttaaataa gggacttgat tatcatttag ttatagataa tataatatta aaaataaagt
    73441 ttaagaggag aaaaaatatg atagatatac ataaaaatat atatgataat aagttatttg
    73501 aggagctaaa gattgactgt aagaagtgct ttggactttg ctgtgtagct ctttattttt
    73561 ctgcttcaga tggatttcct atcaataagg aatcaggaaa accctgtatt aatttacaac
    73621 cagattttaa atgttctgta cataatagtc ttattaagcg aggttttaaa gggtgtactg
    73681 catatgattg ttttggttca ggtcaaaaag tagcacaagt aacatataag gggatagact
    73741 ggatgcaatc accagaacta acaaatcaaa tgtctgaagt gtttttaatt atgagacaat
    73801 tacatgaaat gttatggtat cttaaagaag catctgtatt aaatatatca gatacaatca
    73861 aatcaaaaat tgatttaata attgaagaaa ctgaaaagat aactaatatg ggcccagaac
    73921 agataattaa tttggatatc atatcacata gaacaaaggt taatttatta ttatcacaag
    73981 cgagtgagtc tatgatggga aaggttaaat catttatcaa gacttctact cttaaaaaca
    74041 tgaaaaaatt aagtaaaaat atagatttaa taggagcaga ccttagaaaa ataaatctaa
    74101 taggagcaga tttaagaggt agatttctaa tagcagcaaa tttaagaaat acagatttga
    74161 gtggggcaaa tttaatagga gcagacttaa gagattgtga tattagaggt gctaatcttg
    74221 aaaatagtat atttttaaca caattacaag taaatactgc aaaaggagat tctagtacga
    74281 agttaccagc ttcactaata cgccctaaat actgggaaaa ataattaaat taatacataa
    74341 ttttacaaaa tatgaacttt tttgacttta attcatatta tacattagag attctttaca
    74401 agaatttctt aatataatat agggagagat ataaaaatga gttatcaaaa ttataaatgt
    74461 gaaagagatt atgtgtatga tgattataaa tacaataagt gcaacaagtg ctataatgat
    74521 tatgaggaaa tgacacatgt tcatgaatat tcagaaagtg ttaagttagc agaagaatgt
    74581 gaggatagac ataaccaccg tgcagcaggg gttactggtg aagttatacc aataaatgga
    74641 ggtacaaatc atgttcataa aataaatgat aatgtagatt tccttgacca tttccataag
    74701 atatgtgtta caacagggcc agcaattaga atacctggaa ctgataaaca cattcactta
    74761 atatgtggag aaactactgt taatgatggt cattgtcata aattcctttt tactactcaa
    74821 atagaagcac ctttagtata agctgatgtg aattattgta gtgaatttgc tagattatag
    74881 tataatatat aatagataat atttaaactt aatgaggact atgatattta accttcgtag
    74941 tcctttctaa gttttgtata aaaaagtgtt atgcaattaa aagtgatata gggaggaaat
    75001 ttttatgaaa tctggaataa ttcttgaaat gaaacaaata aattcaacag aatatgacga
    75061 aatgttgcaa gtctgggaat catcagtaag agcaacgcat gattttttaa cagaaaaaga
    75121 tattgaatct ttgataccat tagttgagat tggacttaaa gaagtagaaa atatagtttg
    75181 tataaaagat aatgatataa taaagggctt tataggaata gataaagata aaattgagat
    75241 gctctttata gaagataaat atagaggaaa tggtattggt aaaaaattaa taaaatatgc
    75301 tatagataaa tacaatgtaa aatatgttga tgtaaatgag cagaataaaa aagctgtagg
    75361 attttatatt cacttaggat ttaaagtgtt tgataggtct gaaatagatg gacaagggaa
    75421 cccatttcca atactacata tgaaattaat ttaaagttat acttcccact tattcgcaaa
    75481 aatatttact taaattttta tacaaataaa agtcgtgaca gtcttaggac acgacttttt
    75541 tactacacta ttaaattgaa atttatctgt tctatattat gattattaga tgtagttttt
    75601 ttattttaac tatgtactta aataccaagt atacccatac ctatcaagta cttcagcagc
    75661 gccatcattt tgatgtggtg gtgggtgtgg taaaaattct tgtacaagtg taccaccttc
    75721 ggcaagtttt acagatattt cttctatcgc ttctcgagat ggtagaacaa cacaaatcgc
    75781 catgccagaa attacaacag gcttgtctgt atccgcaccc attatacaac cttcgtattt
    75841 tgtgaaagta acacttgcgt gcataatagg attgtttgga ttgctgtcta atcgcgcaaa
    75901 tatagttttt cctcctccga aagcctcagc ataaaaatta aatgcttttt cacaagtacc
    75961 attaaatatt aaatatggag ttagcatgac caatatatcc tccttgatat agaataattt
    76021 atagttgcac ctgataatct aaattatgat ttagtaaatt gtatcatagg gaaaccttga
    76081 agtatataat tttttattat tatattatac aaatttatta atttaaaaaa atgttatatg
    76141 tacggaaggt ttacaggata aaatataata aaaagattaa ggtgacatta taaaaaatgt
    76201 atattgacat tgacgctacg taattgtata tactttgtct taaaggagat ggtaaatatg
    76261 aatattatag aaattcgctc agataaaata tacaagaaga taatggatgc accaataaac
    76321 aaaaaagaag atatatacag atatgaatta atgaagcctt ttgaatttaa gtggaagtgt
    76381 atgaatgttc caatagttgc tagacagaaa ggtggatatg atgtaattat agcaagtgaa
    76441 atgttagggg ttttatcgcc taaggatatt gatgaaaagc aaaaaaagaa tataaatgtg
    76501 ttatctgctg ataaaatttg gggcacttgc aaagaaacca tagaaaactc tataaatgct
    76561 tttataaaag aagggtatga tttaaacatt aaggactata aatattcaat attattggcg
    76621 aatccaaata gtccttatac aatattaagt gatggatact ggggtgatgg tgggattcct
    76681 ggatatatat ttctatcatt ggttcctaat gaatatacta tcaatagatt accagtatta
    76741 atagcacatg aatgtaatca caatattaga tttcagttta tagagtggaa taataatata
    76801 acattagaag aaatgatgat aaatgaaggt cttgcagaaa attttgcaac atggatgttt
    76861 ggagaggaaa tgttaggacc ttgggtcagt agaacagata tcgaaacatt aaatacttat
    76921 ataaagccaa taataaaaag tgctttaaaa gaaactggat ttcaaaatat aacatcttat
    76981 ctttatggtg atgatatagc taaaatgcaa ggatattttc cagtagggtt gccttattgt
    77041 gcaggatatg cttgtggata ttatatgatt aagtattatt tagaaaagac aaataaatca
    77101 ataatcgaag cgactttatt gccttatagt gagataatcg aagcagtaaa agagttttgg
    77161 gaataaattt tataaattgc ataatttgaa ataaattatg caattatttt atttttaaga
    77221 aaaaataaat tgacaataat ttataagaaa tatacaataa tatatataaa gaaatatttc
    77281 aatattt
//
LOCUS       DAMJYR010000004        69640 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.004, whole genome shotgun sequence.
ACCESSION   DAMJYR010000004 DAMJYR010000000
VERSION     DAMJYR010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 69640)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 69640)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..69640
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.004"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(14..1405)
                     /locus_tag="PC471_000276"
     CDS             complement(14..1405)
                     /locus_tag="PC471_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HDF2787420.1"
                     /translation="MYKLIDKKDIDFLIDTCGEENVLVGSDINEDFSHDELGGIEKYP
                     EVLVNVLETEQVSKIMKYAYKNNIPVTPRGQGTGLVGAAVAINGGIMINLCKMNKILE
                     VDYENLTLTVEPGVLLMTIGQYVQDRDLFYPPDPGEKSATIAGNINTNAGGMRAVKYG
                     VTRDYVRGLEVVLPNGEIINVGGKVVKNSSGYSIKDLLVGSEGTLGIVTKAILKLLPL
                     PKKSISLLIPFPDLSMAIETVPKIIKSKSIPTAIEFMERDVILAAEEFLGKKFPDNTS
                     DAYLLLTFDGNSTEDIEKEYEKVANLCLENGALDVFISDTQERNDSIWSARGAFLEAI
                     KASTTQMDECDVVVPRDKIAEFIRYTHELQDKLKIRIKSFGHAGDGNLHIYILKDGMD
                     DNTWKIRLKETFDYMYKKSRELSGQVSGEHGIGYAKKEYLHESNSDAYMMLIKNIKLA
                     FDPKNILNPGKIY"
     gene            complement(1416..2636)
                     /locus_tag="PC471_000277"
     CDS             complement(1416..2636)
                     /locus_tag="PC471_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HDF2787421.1"
                     /translation="MSKIVVNQDKITDLKRILEICPFGAIEEKSGIVEISAGCKMCKL
                     CVKSGPKGAFEFIESSKVQINKDEWRGIAVYVEHHNGNIHPVTYELIGKAREMASKIK
                     QPVYCVFVGKDIKDKCSNLISYGVDEVFVYDEDEFKDFRIEPYSKAIENFIDKIKPTI
                     VLVGGTTLGRSLAPRLAARFRTGLTADCTILDIQSNTDLDQIRPAFGGNIMAHINTPN
                     NRPQFATVRYKIFSAPEKIENTTGKITLCKVEKKELKSKIKVLSVKEKNKEVGLEEAE
                     VIVVASRAIKKQEDMEMIYKLADKLNAQVAGTRPVIEAGWIDAKKQIGLSGRTVKPKL
                     IITCGVSGAVQFVAGMQGADYIVAINKDDKAPILDVAHLALIGDIYDIIPKLIEKIEN
                     NKDNNQKYMASAAK"
     gene            complement(2650..3438)
                     /locus_tag="PC471_000278"
     CDS             complement(2650..3438)
                     /locus_tag="PC471_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003457975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HDF2787422.1"
                     /translation="MEILTCIKQVPGTTSVEVDETTGVLKRDGVDSKMNPYDLYALET
                     ALRIKEDKKANLKVLSMGPPQAKKVIEESFMMGADEGALISDRRFGGADVLATSYTIS
                     QGIKKMGKVDLIICGKQTTDGDTAQVGPEVAEFLDIPHVTNVTKLIEVKDESIVVEID
                     MPNDLQVCEIEYPCLITVEKDIFQPRLPSFKLKLNTKDREIPVYSLDDFEDKDENNYG
                     LNGSPTQVVRIFPPKPNTDKNIVRGNADELSFALVNKLEELKLV"
     gene            complement(3455..4729)
                     /gene="larA"
                     /locus_tag="PC471_000279"
     CDS             complement(3455..4729)
                     /gene="larA"
                     /locus_tag="PC471_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent lactate racemase"
                     /protein_id="HDF2787423.1"
                     /translation="MVKLKVPYSKQGMILEIPDERFLGVLESKSDNYIEHKTQEEIVE
                     ESMNNPIGSNSLEELVKGKKNVVIITSDHTRPVPSRITMPIILRRIRNVNPEIDVKII
                     VATGFHRPSTREELIYKMGEEIVDNEDIVMHISTDDKSMCKVGILPSGGDLYINKLAY
                     EAELLIAEGFIEPHFFAGFSGGRKSVLPGIASAKTIMYNHCSDFIDSDNSRTGKLNNN
                     PIHEDMVYAAKVAKLAFILNVVIDKDKKIIASFAGDVEKAHTKGCRFVTELSKVNSIK
                     SDIVVTTNGGYPLDQNIYQSVKGMTAAEATCKDGGVIIMISACNDGHGGQSFYENVAN
                     ADSPKQLLDKIRSVDRVDTIPDQWEFQILARILSKYTVIMVTDKCNPEMIKKMHMKHA
                     FNFKEALEMANSIVGKESKVVVIPDGVSVIVK"
     gene            complement(4847..5560)
                     /locus_tag="PC471_000280"
     CDS             complement(4847..5560)
                     /locus_tag="PC471_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HDF2787424.1"
                     /translation="MFNSIASKKVYEQVIEQIQYKILNGELKKGDKLLSERELSEQMN
                     VSRTSIREAIRVLETMGVIESRQGEGNFICTNIEKTLIEPLSMIFKLNNGTLEDILEL
                     RIILEIEIAKLASKRITSSEVIELKHIIDEMRVETNKKDNNRVLVLLDQKFHSKLATL
                     SKNYLIQSLFMTASKLFDGFIEDAREKIIAEPFNENILLKQHEAIYNAVVENDVELAC
                     EKAKEHMDFISKNYRKNEN"
     gene            5685..6944
                     /locus_tag="PC471_000281"
     CDS             5685..6944
                     /locus_tag="PC471_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation integral membrane protein YlbJ"
                     /protein_id="HDF2787425.1"
                     /translation="MKKNKIIAFFAPSIIVGVLVLGIILYPNEAIKSAREGFSIWSNV
                     LVPSLLPFIIGANLIVDLKIVDIIGFIINPITRFVFNVSGKSALAFAISTVSGYPVGV
                     SLASEFRSNGQISKYEAQRLVSFCSTSGPLFIIGSVGTGMFQNSNLGYLMILCHYLGT
                     ILVGLFFRNYGNENLPKTKLDLKTNIKNVINIRNSSGNGFFVLFGNAVFNGVNTLLTV
                     GGFVIVFSVVFKILSLFNVISLIASVIYLPLSFLGVSKELCQAFVSGLFEITIGCNKV
                     SSITSSPEILRASLASFLIGFSGLSILAQCCTFLSKTDIDLKLYILSKFAHGVLAAIF
                     IFILYPIANSAVLVSSFADTYNVIYNNLIWFYYLSYYDTILQIVVIIYLISAVFIAKK
                     HMKTLSTNGKLMRYKNTFFTKKFSRKK"
     gene            7224..7790
                     /locus_tag="PC471_000282"
     CDS             7224..7790
                     /locus_tag="PC471_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDF2787426.1"
                     /translation="MATRPIEIDEYKKIMELLHTGFTYSENGVEKRFRKNPKVALALM
                     LEANLGLRISDILRLKIGNFKGNMLEINEKKTGKLQYRPVNKNIIESINEHARKYNLK
                     SNDYLVNIKTKAIQKQLRIICKYLNLYNISTHSFRKLYATTQFEKSNNNLELVKELLN
                     HSSVATTQRYIRVTQQAINEASENFFIG"
     gene            complement(7991..9196)
                     /locus_tag="PC471_000283"
     CDS             complement(7991..9196)
                     /locus_tag="PC471_000283"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HDF2787427.1"
                     /translation="MKIAILGYGTVGSGLIDLIENNTSKRDIEVVGILVKNKEKHKYK
                     KYFDKITTDIEDIFDKDIDILVEVIGGLNPAFDYVTRALNKKIHVVTANKDLLAEKGS
                     DLIELANLNNVSIKFEASVAGGIPVLKPLIESLEGNNIKSINAILNGTCNFILSKMYD
                     ENLPYDVALKQAQELGFAEVNPDADVLGYDSARKLSILSTLSYGKIVYWKDLLLEGIT
                     SIDEKDIEYAKKLNCKIKLVARSKYESGEVSGFVRPALVDNNNMLSKIDNEFNVVILV
                     GDSVGELSFVGKGAGRGATGSAVYADIIDIIDNRSSNIKSFSKGKLDLSGLIEDECSA
                     VVRFGGYNNKDGILECLDKYVDSYDIIDDEELAIFVSAKSEHEIDRVLEIVKRNNYSE
                     SAKKLLKID"
     regulatory      complement(9299..9405)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            complement(9497..9865)
                     /locus_tag="PC471_000284"
     CDS             complement(9497..9865)
                     /locus_tag="PC471_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbY family RNA-binding protein"
                     /protein_id="HDF2787428.1"
                     /translation="MLKGKQRAYLRSLANTLKPTTQIGKEGVTESFLEQLDGMLRTRE
                     LVKVTILENAGLDTKETANAVCEALRAEFVQAIGFKFTIYKRNIEEPKILFPGHEQAK
                     AKTKNNNVTKKGKPTKRAVR"
     gene            complement(9917..11194)
                     /gene="obgE"
                     /locus_tag="PC471_000285"
     CDS             complement(9917..11194)
                     /gene="obgE"
                     /locus_tag="PC471_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000469293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HDF2787429.1"
                     /translation="MFIDKARIFVKAGNGGNGSVAFRREKYVPAGGPDGGDGGRGASI
                     IFEVDLGLRTLMDFKYQKKYQAQNGGDGSKGKRAGKNGENLVLKVPAGTVIRDEATGL
                     VLADLKKEGDTAIVAKGGIGGKGNQHFANAVRQAPAFAKSGTDGEERWITLELKMIAD
                     VGLLGFPNVGKSTFLSVVTKAKPKIANYHFTTLTPNLGVVQTKFGDSFVLADIPGIIE
                     GASEGIGLGHEFLRHVERTKVLIHIVDISGLEGRDPIEDFDKINDELKLYNEKLSKRP
                     QVVVANKFDILEDESKFEKFKSELEGRGYTVFKMSAATRQGIDEVIAYVSKMLKEVED
                     VELVSEEEMYRPELDIGTEEELSIDIEDGVYVVTGKALRRIMYSVNFDDMESLQYFQK
                     AMESQGVFDRLREMGIEDGDVVKIYELEFEFYN"
     gene            complement(11305..11595)
                     /gene="rpmA"
                     /locus_tag="PC471_000286"
     CDS             complement(11305..11595)
                     /gene="rpmA"
                     /locus_tag="PC471_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HDF2787430.1"
                     /translation="MLNMNLQLLASKKGVGSSKNGRDSISKRLGVKRFDGQLVTAGSI
                     IVRQRGTKIHPGTNVGKGSDDTLFALVDGTVKFERKDKKRKKVSIYPVAIAE"
     gene            complement(11609..11926)
                     /locus_tag="PC471_000287"
     CDS             complement(11609..11926)
                     /locus_tag="PC471_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HDF2787431.1"
                     /translation="MIKVKYYYNDDFLLQGFCLKGHADFAEIGYDIVCAAVTSNAIAV
                     INSLDKLQKIEFEKVVGEEGHIECIVKDTHVKDAQLLLNHFQLAVKEIKREYPKNIKI
                     LKK"
     gene            complement(11941..12252)
                     /gene="rplU"
                     /locus_tag="PC471_000288"
     CDS             complement(11941..12252)
                     /gene="rplU"
                     /locus_tag="PC471_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012063435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HDF2787432.1"
                     /translation="MYAIVKTGGKQYKVSEGDVLFVEKLEANAGDVVTLNEVLACSKD
                     GELKLGSPVVEGASVQAKVVEQGKAKKVIVFKYKAKKDYRRKQGHRQSYTKIVVEKIN
                     A"
     gene            complement(12353..13774)
                     /locus_tag="PC471_000289"
     CDS             complement(12353..13774)
                     /locus_tag="PC471_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HDF2787433.1"
                     /translation="MKKIVIESLIGSQKTAVLEDERLTELFVEDNLNKKTVSNIYRGI
                     VKKVIPGIEACFVDIGFKKLAYLQLKKGSTIKSGQDILVQINKEEIGTKGAKLNTEIS
                     ISGRYIVYIPSNDRTTISNKITDEKERFRLKKITKAVNKENLGLIIRTEAQGCTHDEI
                     KKDIEELKLKYENILKEYKLGIGPKLLYKSLDFATKYVKDNVNDDIESIITNSYDKYS
                     ELKSILRGIDKTYVDKLCLEENRDVFDLYRIESKIEKLLNKKVWLKSGGYLIIEKTEA
                     LTVIDVNTGKFIGTGKLDETVYKTNLEAAKEIVRQLRIRDIAGIIIIDFIDMHKKKHQ
                     KEILHILEEEFNKDKRKAEVLGMTKLGLVEVARRREKESIDKYYLMSCPCCDGEQTIK
                     SVHYILDSIEKEIMRISEHTVYKNIMVEFNDFIFEQIKEYYMDIIDKIGEKYNIKISL
                     NANSTLKHNKTNVIFDKIVDNKM"
     gene            complement(13792..14493)
                     /locus_tag="PC471_000290"
     CDS             complement(13792..14493)
                     /locus_tag="PC471_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2344 domain-containing protein"
                     /protein_id="HDF2787434.1"
                     /translation="MSKIIRVKFKKEGDMIYISHLDLQRLLQRAFRRAEINLSHSQGF
                     NPHPKMSYGNALALGAESQGEYVDIEIEEDDLSVEEFLNKVSIQLPDGIDFIKAKEID
                     RQTPSLSSVIDYGEYLFNIDLKRPMTKEFVKRKVIDFMNNKEIIITKKNKKGKMVEVD
                     IRPMIRTFDVLNLEDEHITLTATIATGSKTNLNTNILIPKILEMFELDIDPLDVDILR
                     RDLYVLEDGELVTPM"
     gene            complement(14495..16339)
                     /locus_tag="PC471_000291"
     CDS             complement(14495..16339)
                     /locus_tag="PC471_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03960 family B12-binding radical SAM
                     protein"
                     /protein_id="HDF2787435.1"
                     /translation="MNKVDLKKILKKVEKPARYLGNEINSIHKDTSNSELIRYAHCFP
                     DLYEVGMSHLGSHILYDVINKDEDVFCERVYSPAVDMENIMREKSIPLFALESREPIT
                     NFDFVTFTLQYELSYTNILNILDLANIPILKEERTLEDPFIMVGGPCAYNSEPLADFV
                     DVVILGEGEEVNLEVVNEYKEWKKNKTTREDFLYKISSIEGVYIPSFYDVKYNEDGTV
                     QSVTPNREGITKNPTKRIIKDVETVDYPEKLIVPYIDTVHDRIVLELFRGCTRGCRFC
                     QAGMIYRPIREKSVKRLKEILDKLVKNTGYDEISLSSLSTSDYSKLSELTDYLVDEYA
                     SNNIGISLPSLRLDNFSMEIADKIQQVRKSGLTFAPEAGTQRLRDVINKGVTEEDLEN
                     ATERAFEMGWNSVKLYFMIGLPTETYEDLDGIAKLAYKVIDIYRKVNGGKLKRSFSVT
                     VSTSTFVPKPFTPFQWHGQDTTEEVINKQRHLVNKLRNNNIKYNYHDSKTSLMEAVVA
                     RGDRRIGKVIYDAFRLGAKFDGWAEHFNLDIWKEAMEKNNLSIDFYVHRNRNYEEVFP
                     WDHIDVGISKKFLIREDENAKKEKITSDCRHNCNGCGINIHDIGRGLC"
     gene            16658..17851
                     /locus_tag="PC471_000292"
     CDS             16658..17851
                     /locus_tag="PC471_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FprA family A-type flavoprotein"
                     /protein_id="HDF2787436.1"
                     /translation="MSKVFEVKKDIYFTGVVDEGLKVFDIIMETEFGTTYNSYLIKDE
                     KTVLFDTVKANFKDEFLSNLSEVTDIAKIDYVVIHHTEPDHAGSLKYLLDINPNIEVY
                     CTKAAKLYLDGQINRPFNCHVIKDGEILNIGKRNLRFITAPFLHWVDTMFTYIEEDKT
                     LLTCDAFGCHFASVDAEVVNSEDYLKSAKHYYDCIVKPFAKHVLSAVDKVVGLNIEFD
                     TILTSHGPMLTKDPMAAVKRYVEWSTEAVNTTNQNQVSIFYLSAYSNTLEMAKKIKEG
                     LDKEGAKAELYDLEDMTLTEMHDTLVVSKVILLGSPTINKTMVKPMWDLFSVIDPMAN
                     QGKIAGVFGSFGWSGEGITMAETLLKSMSFKMPVESLKKKFFPSEETLKECMAFGAEF
                     AKLVK"
     gene            complement(18063..18883)
                     /locus_tag="PC471_000293"
                     /pseudo
     CDS             complement(18063..18883)
                     /locus_tag="PC471_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428476.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
     gene            complement(19013..19804)
                     /locus_tag="PC471_000294"
     CDS             complement(19013..19804)
                     /locus_tag="PC471_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2787437.1"
                     /translation="MKLIDEYLDKLYKKCDNKSTIELKQEMRCHLIESANEFKLEGLD
                     EEEACKKAIERFDDGDEMQYELCNIIKELSLSLDRHKSIVMGFKKVLGYISIIAFLIS
                     GFMWYYNNSLQHNMYNLGKELDGEIKQLAERHDMTKIGEYKLELEKILDKDKYSKVKA
                     LRLYVIDMKDGNTNLSSSGLNANMVYEREADYNNISNFIQHLGYNGKDFLDKNGNIVN
                     PDIFLEYFFYFESEMLIPVAFVFGLLCIIAYFILRFKISLIKNNN"
     gene            complement(19808..20137)
                     /locus_tag="PC471_000295"
     CDS             complement(19808..20137)
                     /locus_tag="PC471_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2787438.1"
                     /translation="MQLNKEVLKGYIDILIVSILEKKDCYGYEIAKQVRERSEFELKE
                     GTMYLALKRMESKNLIKSYYSNEQSSGGRRKYYNLTNEGKDFLEIKKQEWRFIKKVMN
                     QFLGEVD"
     gene            complement(20622..21035)
                     /locus_tag="PC471_000296"
     CDS             complement(20622..21035)
                     /locus_tag="PC471_000296"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HDF2787439.1"
                     /translation="MNIALVAHDQMKNTMVGFCIGYESILKKYGLYATGTTGKRIMDE
                     TELNINRLASGPLGGDQQIGSLIVTQEIDLVIFLRDPLTSQAHETDIQALIRLCDVYH
                     VPIATNLASAEIFIKALDRGELSWREVRKSKSQRI"
     gene            complement(21076..22206)
                     /gene="rodA"
                     /locus_tag="PC471_000297"
     CDS             complement(21076..22206)
                     /gene="rodA"
                     /locus_tag="PC471_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HDF2787440.1"
                     /translation="MKKININYKSTIKLIKQLDWKLIVTVLAIFIFGLVILSSATHAN
                     STGSYNQLIKQGLAFVLGIGMIIVILFFDYNLLGRYYKALYIISLILLAIVLLPGIGT
                     VKGGARSWINLGPLDLQTSEIVKLTFVLSYAKILESKKDKLNTLKEVMPVVVYSLPFI
                     GLLIAQPDLGTGIVFCCMIFAMLFTAGLSSKLIKRGIIILLVSMPLMYLMMADHQKVR
                     IEAFLNPEDVTLKGNYQVMQSLIAIGSGGVTGKGLYNGSQNQEDFLPVQDSDFIFAVV
                     GEELGVIGMAVLIILFMIFLLRLLAIARDAKDFYGTLIVVGVMGMFGYQIIQNIGMTV
                     ALIPVTGVTLPFVSYGGSSLLTSLANLGLVLNVCMRRKKINF"
     gene            complement(22291..22575)
                     /gene="minE"
                     /locus_tag="PC471_000298"
     CDS             complement(22291..22575)
                     /gene="minE"
                     /locus_tag="PC471_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HDF2787441.1"
                     /translation="MLDLFRVFSNEAKTSKSVAKERLKLVLVHDRVDCSPQLLEMIKT
                     DILKVIANYAEIEDDGLEIKMSKCRGEHDDKPVSALVANIPLKNIKDRCM"
     gene            complement(22593..23390)
                     /gene="minD"
                     /locus_tag="PC471_000299"
     CDS             complement(22593..23390)
                     /gene="minD"
                     /locus_tag="PC471_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HDF2787442.1"
                     /translation="MSEVIVITSGKGGVGKTTTAANLGTALSLENKKTVVVDADIGLR
                     NLDVVMGLENRIVYDIVDVVEGTCRLKQALIKDKRFDNLYLLPAAQTRDKNAVSVEQM
                     IDLCEKLKESFEYIIIDCPAGIEQGFKNAVAGADRAIVVTNPEISAVRDADRIIGLLE
                     ANEIKEIRLVINRIRNDMVKRGDMMDKQDIIEILAIDLLGLVPDDESIIISTNKGEPA
                     ILDSKSLAGQAYKNIAKRILNEEVPLLDLEVEDGFFGRLKKMFSMAK"
     gene            complement(23414..24097)
                     /locus_tag="PC471_000300"
     CDS             complement(23414..24097)
                     /locus_tag="PC471_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HDF2787443.1"
                     /translation="MSLREICSQELVEFKGNKRGIIVNIKREAPFEEIQEKIINKLEA
                     YVGFFNGAKISKINSDCLTDMEILELKEGITSRFDVEFVEDQKIEENSNFPTKYVNTL
                     RSGENIEFEGDVVILNDMKPGSKVLSKSNTVVMGDINAGAKVVAGGNVFVMGKIEGFV
                     HAGAEGNEFAYVVAGNLNPKILQIADNIAEAPDDEENYESESEISPEIAFVSNGRIVI
                     ESYLSKLDK"
     gene            complement(24195..27173)
                     /locus_tag="PC471_000301"
     CDS             complement(24195..27173)
                     /locus_tag="PC471_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HDF2787444.1"
                     /translation="MEENKQVDRLKIIKIVMGVIFLAILVKIIYMTTFKYEYYNELAE
                     NKTYKKLAIEAPRGEIKDRYGRLLAGNKNLFTVQVSGNDINKKDANKHSRANEISLKL
                     INLLERNGEEYVDEFPIYVENGKYYYTYDRDIREYKSENGIPNDYNAKESFYYLVDKL
                     ISAGILSQEDKRLDATRLQAKLNENGYYPPILVSKWMFTAERDKRDWLASYKIKETKL
                     SAKEAFEKVRNSDALEIDKSLSDEDARKIMVVRDLIKSKGYSQYNPVTIAKDVGETTI
                     AQIEESAMDLVGVSIAVEPVRYYPNGSLASHMLGYVGKMPSTQIESYLQKGYETGDMV
                     GLAGVEKSNESRLRGTDGYKMVKVDALGRISKEIESKKPKSGDTVYLTLDKDLQEVSD
                     NALKQIIEVASKGGTFKSKFGDKPISAYAGKAQSAALIAIDVKNGEVLASSSYPNYDP
                     NKFAKGISTEDYKALQPKNPNDLLAGSPLLNLVTQGEFQPGSSFKMVTSMAALENGLD
                     PNFTINDPGVIMLGKKSFGDYVWNHGRGNHGMTNLYKAIQESCNIYMATIGTGKTWPD
                     GKSIGIDMNANKILEYAKLFGLDQNTGLQDEVEERAGKVPSTEDKLKSTQALLKSNLE
                     REMANDFVDITREKNPKEYEKRINEIVSWAAEKKTPGRVETMNRLKKLDVKEDRIEEV
                     ADLAVFSYFNFAKWSTADTFNLAIGQGENAYTPAQISRYVAAIANGGNLVELSVVDRA
                     VSSDYSSVKINDQKKVEKIPFKNPDNLKELTKGMKLVARQGTAKSAFADFPIDVAAKT
                     GTAEKSGKIPTDNEYEYLKSHMSSYNVNLNDAIKLADKMKAEKEKELSLAKEKEIKKK
                     LENKDLKDEERKKLEEELEDGVKVRLEDTDKVNSSYLRKAIKELNPKITDDQIDRFKQ
                     DYGSFTWTVAFAPADDPEIAVVCVIPQGDSSVFSLLPTREVIGTYMGLEPTNSKNDNK
                     TNDVNNSSDENINFESQINR"
     gene            complement(27185..27673)
                     /gene="mreD"
                     /locus_tag="PC471_000302"
     CDS             complement(27185..27673)
                     /gene="mreD"
                     /locus_tag="PC471_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HDF2787445.1"
                     /translation="MKKVLLCLLGIVLMTIENSITNYIDIFGISFNLVLIYVTIISLY
                     LDELEGGIIAAIIGLIKDITVGGIFGVNALILFIIAYAIGYMRDQLYKGSYIITFVLI
                     FIGSLIDSIVNIGTSSIIYQSYNISTLFVKGLLVAPIVNSLIGLVIYRLSKRAVLKLK
                     ED"
     gene            complement(27688..28581)
                     /gene="mreC"
                     /locus_tag="PC471_000303"
     CDS             complement(27688..28581)
                     /gene="mreC"
                     /locus_tag="PC471_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HDF2787446.1"
                     /translation="MALRFDKNKNEKKRINVKVIATGVVAITLIGIVGISIGKFSSGS
                     PVNLGVASDAITSVGKGINDGFSFIKNGFKDVANFKDNSKKVKKLEEENEKLKKNMIA
                     LNAKLDKTESLEELKKTLNFVQEEYKATSISASVVGKNDGNWYESFVIGAGKNSGVKK
                     ESIVMNGSGLVGIVYEVSNNYSKAISLLDSKASVSFKLAKDANAKGTITQNTTLDNKD
                     SYNSKGYLQGYMFDSSYNVIQGDIITTSGLGFFPDGIPIGEVEKVVDDKDKSLKYVVV
                     KPYVDFKNINDVVVIEPRNIG"
     gene            complement(28591..29601)
                     /locus_tag="PC471_000304"
     CDS             complement(28591..29601)
                     /locus_tag="PC471_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HDF2787447.1"
                     /translation="MTKDMGIDLGTANTLVYIKGQGIVVREPSVVAIRDDSKEVLAVG
                     EEAKKMIGRTPGNIVAIRPMKDGVIADFDITQSMISYFIQKAADKKGVVSPRIAICVP
                     FGVTEVEKRAIEEAARQAGAKDAFLIEEPMAAAIGAGLKVEEPEGNMVVDIGGGTSEI
                     AVISLGGIVTAKSIRIGGDEFDESIVAYVKKEYNLMIGERTAENVKINIGSTFKDDEE
                     INMQIRGRDLISGLPKTIEICSTEVREALKEPVSSIVDAIKSTLERTPPELASDIMEN
                     GIMLTGGGALLRGLDKLITQETGMSVQIAETPLDCVALGTGKSVEDQEIFEKVLMMNT
                     KN"
     gene            complement(29722..30381)
                     /gene="radC"
                     /locus_tag="PC471_000305"
     CDS             complement(29722..30381)
                     /gene="radC"
                     /locus_tag="PC471_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HDF2787448.1"
                     /translation="MAPEERPREKMLAKGVKSLSNAELLAILLRTGNKNKNAIELANY
                     IINRDIQGIRHLEDMTIEELCNIDGIGLSKSTQIKAALELGSRVASFKPIKYKIRNPW
                     DIQRYYMDSLRYLKKEVFKAVLLNTKNEIISDVDVSIGTLSSSLVHPREVFKEAIRRS
                     ASKIIVMHNHPSGSVEPSREDKNITSRLIKCGEIIGIEIIDHIIIGDGLYFSFKENMI
                     I"
     gene            complement(30426..31010)
                     /gene="maf"
                     /locus_tag="PC471_000306"
     CDS             complement(30426..31010)
                     /gene="maf"
                     /locus_tag="PC471_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
                     /protein_id="HDF2787449.1"
                     /translation="MNIILASASPRRKEILENTNVRFDIIKNEIDEIILEGETPKHLV
                     MRLAFEKSMSVASEHNEDVVIGADTVVVLDNAILGKPKDESCARDMLKRLSGREHQVI
                     TGISLINLCEDKKVIDYVISNVKFKTLSEQDIEDYLKTNESFDKAGAYGIQGYGALLV
                     EEIRGDYFNIVGLPISRLGDLLKKYFSINLFYGV"
     gene            complement(31230..32201)
                     /locus_tag="PC471_000307"
     CDS             complement(31230..32201)
                     /locus_tag="PC471_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit B"
                     /protein_id="HDF2787450.1"
                     /translation="MVILTAVLVLGIMGLIFGIVLDFASKKFAVEVDERVEAILGVLP
                     GANCGGCGFPGCGGLANAIVEGNAPVNGCPVGGADVGAKVGEIMGISAEAGEKQVAKV
                     ICKGTCSSAKDKYEYEGISDCRAANVLNSGAKMCKFGCLGLGTCKDACKFDAISIVDG
                     IAVIDEEKCVNCGKCKEVCPKGIIITKPESQEVVVECNSKEFGKAVKEKCTAGCIGCG
                     MCVKACKFDAIIFEDKIAKIDPNKCVGCMQCVAKCPTKVISGDITKKKKVTIDQELCV
                     GCTVCKKQCKFDAIEGELKEKHKVDADKCVGCHLCMEKCPKKAIKIL"
     gene            complement(32217..32792)
                     /gene="rsxA"
                     /locus_tag="PC471_000308"
     CDS             complement(32217..32792)
                     /gene="rsxA"
                     /locus_tag="PC471_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HDF2787451.1"
                     /translation="MNLILLFLSIVLVNNVITSQFLGICPFLGVSKKVDTAVGMGVAV
                     TFVLTLASIITYFIQILLVKTGTGFLQTIAFILVIASIVQFVEMVIQKMSPSLYQALG
                     VYLPLITTNCAVLGIALVNVQKSYNLVETIINGFGAGAGFTLAIVIFAGIRERLELAD
                     IPEAFKGFPITLISAGLMSIAFLGFTGLIKL"
     gene            complement(32807..33400)
                     /locus_tag="PC471_000309"
     CDS             complement(32807..33400)
                     /locus_tag="PC471_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit E"
                     /protein_id="HDF2787452.1"
                     /translation="MNLAKVFKNGLIDENPTFVQVIGMCPTLAVTTSAINGIGMGLST
                     AAVLICANLVISLIRKITPDKIRIPIFIVVIATFVTIVGMLLKAYVPALDKALGIYIP
                     LIVVNCLILARAESFAFKTGAMPSIVDGVGQGLGFTVALTIIGAVRELLGNGSLFGMT
                     LFGASFQPVLIFILPPGAFLTLGFLFAGFNKLRSKKA"
     gene            complement(33413..33982)
                     /locus_tag="PC471_000310"
     CDS             complement(33413..33982)
                     /locus_tag="PC471_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit G"
                     /protein_id="HDF2787453.1"
                     /translation="MNSMVRLGGTLLAISAIAALALGATNQVTAPVIEQRNIQANNEL
                     RKAVLPEAKEFKEMKEDTYKGLGDGLIAEVYEGLDGSEVVGYTLKAKPSGYGGEIEVM
                     VGISSEGQVTGVDIGNMSETAGLGAKAKDDAFKGQYKGKTAEPLEVAKGSTTADNQIL
                     AISGATITSTAVTTGVNAAIDVFNSALNK"
     gene            complement(33982..34959)
                     /locus_tag="PC471_000311"
     CDS             complement(33982..34959)
                     /locus_tag="PC471_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HDF2787454.1"
                     /translation="MENKLIVSSSPHVRSNEDTSYIMKQVIIALLPAAVAGVYFFRLN
                     ALSAMFFCILGTVGTEFLYQKFTKQKSTIGDFSAVVTGLLLAFNVPASLPWWMCLVGG
                     IFAILVVKMVFGGIGCNFVNPALAARAFLLASFPVAMTAWTQPGVNWIGKNLDAVTTA
                     TPLSFLKNGAAGLADLSSNGISLADMMIGNIGGCIGETSAILLLLGGVYLMYKGIINY
                     VIPVFYIATVFILTFLLGGFNITFAIYQLFAGGLMLGGFFMLTDYTTSPMTKKGQIIY
                     AVLAGLITTVIRMYGGYPEGVSYSILLVNCLAPLIDKFVRNRVFGEVAK"
     gene            complement(34982..36319)
                     /gene="rsxC"
                     /locus_tag="PC471_000312"
     CDS             complement(34982..36319)
                     /gene="rsxC"
                     /locus_tag="PC471_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HDF2787455.1"
                     /translation="MKLLTFKGGIHPPYRKEYSNTKALEKAQAPKIVYIPLQQHIGAP
                     AKPIVEVGDEVKFGQKIGEQQGFVSCNVHSSVSGKVIAIEQHEVPGGSAQCVVIENDF
                     KEELHESVQPKGQLEDLSKEDIVGIIKEAGIVGMGGATFPNHVKVSPPPDSKAEVVIL
                     NGAECEPYLTADHRLMVENPEDVVFGLRALMKVLDVKKGFIGIETNKPDAIEAIQNVA
                     KDYSEIEVVGLQVKYPQGAEKQLIYACTGKEVPSGGLPIAAGAVVDNVATAAQIAKSI
                     KTGMPLVERITTITGSCIKEPKNLITKVGTLVSEIIEQCGGFKEDKKIGKVIMGGPMM
                     GIAQYTTEIATNKGSSGILCLDEEESRTPDIQNCLRCGRCTDVCPSFLQPLFISAYSL
                     KDDYDTAEYHRAMDCIECGSCSFICPARRPLLQSIRSAKREIGAKRRKQAAQK"
     gene            complement(36535..37479)
                     /locus_tag="PC471_000313"
     CDS             complement(36535..37479)
                     /locus_tag="PC471_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2787456.1"
                     /translation="MNRRKTIYRGFNNNRHKIDLRKYVITIACLCLIGYYSYTKIKDS
                     KILEYVSAKIPFLNNSSDITYKDISDELNSIKKGKKSKSQTNSDDKQETNPEKAVNNT
                     KEPEEVKLATIEGWDMYTIQVAAIDNNDDLKKIQTSLVNNDIPFSVMEKDGVKKIQTY
                     SSFDENDVRKQISSVRKVFPDAFLSHLDAPMLSLEYTSNYAYIESISKELNKLITNFK
                     EESSFWSNAENNVDMEKYNTILTNRKAISQNISKEAEKIDYSEMRLFKDNLIEYVKNV
                     NEKIDTASKAANEEKYSVSKSLLLSSMQEYSMFINSIK"
     gene            complement(37590..38864)
                     /locus_tag="PC471_000314"
     CDS             complement(37590..38864)
                     /locus_tag="PC471_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HDF2787457.1"
                     /translation="MKKAIAALGIGAVAVSVSSINASALEKGTVTASALNIRSGPSSD
                     CDKVAKLYKGKTVEILEKSNGWYKVRVSSSVVGWGSAKYISTSGSSEGTSSQNNPTSS
                     GTTISGNGKVNVSSRLNVRSGAGTNYSLVGKANNGDVVKLLEQSNGWYKIKLSNGVTG
                     WASSQYISKTSEDVGTNNSSNSNSTNNSDKKPSSEESIEGKNGKVTSAVSLNVRSGPG
                     TSYSIIGKLNGGDVVELKAKSNGWYKVKLSSGTIGWVSASYISETNEDTKEKPNSSSN
                     QNSQSNSNSKPSFTGNSDKSTAKGSTIVDFAYTLIGIPYQWGASGPDKFDCSGFTQYV
                     FKHSVGVSIPRVSREQANFGSAISMGNYAPGDLVYFDTDGDGTTNHVGIYVGNSKFIH
                     CSGTQTNPNKVKVDNLTSSYWSKVLLGARRFV"
     gene            complement(38996..39367)
                     /locus_tag="PC471_000315"
     CDS             complement(38996..39367)
                     /locus_tag="PC471_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDF2787458.1"
                     /translation="MKFNFCHNNFNVTNLEKSLDFYKKALGLKEVKRKEAEDGSFILV
                     YLGDGITSHTLELTWLRDWDRPYNLGDNEFHLALEVEEFDEAKKLHKDLDCICFENES
                     MGIYFIADPDNYWIEILPKNH"
     gene            complement(39595..40194)
                     /locus_tag="PC471_000316"
     CDS             complement(39595..40194)
                     /locus_tag="PC471_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2787459.1"
                     /translation="MESRGYKEDLKKMILDMAHKELESYVSQKGAKKYFEGNLDKIIN
                     NNIKKFNQSIGLNRVYLKMLKKCAYQESVTSISRTIKKEALFKSKKELFAFSEYLKLD
                     INKRLSYNQILRKISRYIYYNRSSYAQKYVVFKRGDEEYLLEPEKIKDELISSYRSKT
                     REDMRSIAKLLDIEVEDDYNAEDIRKKVINYIIKEKLKS"
     gene            complement(40395..40607)
                     /locus_tag="PC471_000317"
     CDS             complement(40395..40607)
                     /locus_tag="PC471_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HDF2787460.1"
                     /translation="MKKKLLVEGMSCGHCVNHLKTALTEDIDGIEVLDVDLENKCASV
                     DMKDDVSIEKLKEVIAELGFELKGIE"
     gene            complement(40680..42395)
                     /locus_tag="PC471_000318"
     CDS             complement(40680..42395)
                     /locus_tag="PC471_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDF2787461.1"
                     /translation="MKRIKVLTVVLAITLMVAGCSNTKTKQSSSDSSLQSQSSIDAKY
                     INLTMVTPKTINPITNTDKSVGYIMNLVYDSLFTIDENYNVIPQLVKEYNIAQDGMSI
                     DIKLKDAKWHDGKNVTSNDVNYTIGLIQKSADSPYNEFTKNIASVNIKSDKDFTIKFK
                     ARYAFSIDSLIFPIVSQNHLDSKDVNDKNNNMIGNGKYKIESYTEREGMVLSVNKDYY
                     EEVPKTMKNIKVGMVPNEDARTSMVMALDSDITNVTLNDLSKFQEKEFNITKYQGRDY
                     ECVLFNYNNPFFKDVNFRKAIAHSIDKDRIISEGYMDDATPVNFPLNSKSKYYNSEMK
                     DLEFNKDKAIECLAKVEYANVNSVNQNDNKVKNQKKSAKKNSKKLTPEEEAKVKKAEE
                     DRIKKEAEEKKNREKEEVKKSLSEMNLKIIVNRDNSERIKTANIINENLKAIGINSTV
                     NQLSDKDMENALNSKNYDLALVGWKLSIIPDASSIIASSGYTDDKLNGYMSALTSSTS
                     EIETKKAYKDVQTYIKDNATFISLAVRNNYIVSNSRLKGKITPNDFDVYEGISNLDIK
                     SNESN"
     gene            complement(42395..42949)
                     /locus_tag="PC471_000319"
     CDS             complement(42395..42949)
                     /locus_tag="PC471_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HDF2787462.1"
                     /translation="MNSKKVLILSIFIILFGALLMESKVIHKFLYPKKYSEYVEKYSK
                     EFNLDENIVYSVIKAESKFNSSAVSKKEAKGLMQILDITRDWGAEELNLKNVDIFDPE
                     TNIRLGCWYLSKLYKEFGKLDLVIAAYNGGSGNVKKWLENNEYSKDGENLHDIPFKQT
                     SKYVEKVKNNYEHYNKIYGKKGKN"
     gene            complement(42942..43544)
                     /locus_tag="PC471_000320"
     CDS             complement(42942..43544)
                     /locus_tag="PC471_000320"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HDF2787463.1"
                     /translation="MLILGLTGGIGCGKSSLSNIFRNLNIPIVDADIISRKIFEDKLL
                     LEKVFVHFGQSIKNDDGTLNRKALGKIVFSDEEKLKELNNLTHPRIREKIISEIEKLR
                     KKGENIVVLDAAILVESGFLDMVDKLLVVTCKQEVQISRIQKRDNCSEQEALSRINSQ
                     MSQEEKSKYGDYIIDNSGTITELESKAHKFIEYMKENWRE"
     gene            complement(43553..46201)
                     /gene="polA"
                     /locus_tag="PC471_000321"
     CDS             complement(43553..46201)
                     /gene="polA"
                     /locus_tag="PC471_000321"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HDF2787464.1"
                     /translation="MEKKLIIIDGNSIINRAFYALPEMNNKEGLKTNAIYGFTTMLFK
                     MIDIYKPTHISVAFDRKAPTFRHLEYKEYKAGRKGMPDELAEQLQPLKDLLDKFRINR
                     LEIDGYEADDIIGTVSKKAEENGYKVYIVTGDKDAIQLASDNTTTLITKKGVGEVEEY
                     NFNSVIEKYEMTPTQFIDLKGLMGDKSDNIPGVPGIGEKTGIKLIKEFSSIENLIENT
                     DKLKGSVKKKIEENKEIAIQSKRLATIIRDVPIEVDLDSMIFGDYDKDELLDKFRYFG
                     FTSLLSRLVDLVDSDDTEHVEEKIEILKLKDIEKFIDEVNKKGQVILKTVRGQGNILE
                     KNIIYIFISVDGEKIYYVDSNEVSKLDSIFSNNEIKKYGYNLKDDYISLKPYNINLEN
                     MYFDITIAEYLIDSTSSTSYDCSAIAMKYLSKKVKSKEELLGKGVKSKNYEDLEFEDL
                     AEYMGQIICTVKETVPMMERSLMDMSMDGLFYHVEMPLVEVLGHMEYEGIKVDKSILD
                     ELSVEFKEIIATLEKEIYELSGEPFNINSPKQLGVILFEKLELPIIKKTKTGYSTNAD
                     VLEKLRDKHPIIDKITEYRQIVKLNSTYVEGLLNIINPESNRIHSSFNQTITTTGRIS
                     STEPNLQNIPIKMEMGRKIRKVFIADDNCKLVDADYSQVELRVLAHMSQDENMIEAFK
                     HHEDIHTKTASQVFNVPMEDVTSELRGAAKAVNFGIIYGKSDFGLADDLNIPVPKAKE
                     YIESYFAKYHKIKEFMDTTVEKASEDGYAVTILNRRRYIPEIKSSNFMERNRGKRFAM
                     NAPIQGSAADIIKIAMINVHKKLEENNLKSKLILQVHDELIVEAIDDEIDIVKKIVKE
                     EMENAVNMDVHLDVDLNVGSSWYETK"
     gene            46353..46440
                     /locus_tag="PC471_000322"
     tRNA            46353..46440
                     /locus_tag="PC471_000322"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:46387..46389,aa:Leu,seq:caa)
     gene            complement(46627..47070)
                     /locus_tag="PC471_000323"
     CDS             complement(46627..47070)
                     /locus_tag="PC471_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2787465.1"
                     /translation="MKKNMLYVGIIYFICGILLVLLATFTEFSFEAFIWGLSGAALGP
                     GVCMILQYMYWSKPERAVDYEEKIKNQRIEMNDERRIMLRDKAGRITNQIMSYVLVIL
                     IFIVSILSVFSVMVISKWVLVVLGLLILFQFICSVVVYNKLDKKL"
     gene            complement(47073..47270)
                     /locus_tag="PC471_000324"
     CDS             complement(47073..47270)
                     /locus_tag="PC471_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004631111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2787466.1"
                     /translation="MKNRLEEIRKERGIKQEELASILQVSRQTIGSLENGRYNPSIIL
                     AFKIARYFDMSIEEIFIYEEE"
     gene            complement(47462..48328)
                     /locus_tag="PC471_000325"
     CDS             complement(47462..48328)
                     /locus_tag="PC471_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HDF2787467.1"
                     /translation="MEWINKLNQAITYIEENLESGVDYAEAAKIACCSTFHFQRMFSY
                     IAEVPLSEYIRRRRMTKAAFELQNSNIKILELSEKYGYDSPTSFNRAFQNIHNISPSA
                     ARAKGVVLKAYPKMTLSIYVKGNIEMQYRIVEKKGFRIVGIKESMNMIVEECFEKVPK
                     LWAKCIKNGTIDKLSELINNEPCGLLGVSVCTNSKGLDYYIAAPTDKPVLEDTHEYLI
                     PSGTWAVFECVSPMPNALAIQELQKRIITEWLPSSGYVYANLPDIELYPNGDINAPDY
                     TTEVWIPIQKPS"
     gene            complement(48554..49423)
                     /locus_tag="PC471_000326"
     CDS             complement(48554..49423)
                     /locus_tag="PC471_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HDF2787468.1"
                     /translation="MDYKTIILNRKSVREYKNTDIKEEYLSEIKKYSESCKKLVPEID
                     VDVRIMNRSEVFNNLDKVAGYKGNMIDSPSYIIILSDIKDNYIENSGYIGESIIFKVT
                     ELGIGSCWVTFEDGKKVKEKLGINSDKEVTGIIAIGYGENVSNAKVLNATKTGQNYSK
                     SDMQVVTDKGSTRLGVEDVVYMNEWGNVASIEILEERALLDAFHYARLAPSTLNRQPW
                     RFIVDGGTVVLAVRKDGHTNLYEEKIDIGIVMLYFATIISATMFDLKWNLGTPDKDYK
                     VPEDYKIVGYCNI"
     gene            complement(49929..50150)
                     /locus_tag="PC471_000327"
     CDS             complement(49929..50150)
                     /locus_tag="PC471_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2787469.1"
                     /translation="MNVNNHKCPECGEHSKKFEIVEENEDCVIFECYTCGTISILKYC
                     ENCKEFVFVNIPNNVETYEYCCGKCNNKI"
     gene            complement(50651..51631)
                     /locus_tag="PC471_000328"
     CDS             complement(50651..51631)
                     /locus_tag="PC471_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2787470.1"
                     /translation="MGDTSRFYESVKNKYPKLKDGTRVHLWPKENIIIGPIYDEEDGY
                     KKSIGNVDSLSWKQIVILAECDGKNTTDDIINILSNRYRNIHNMKEKVMEFFMFYENV
                     YLTFEDDIQTLSSVFEITGNKSYITPLYFTIEIDGDNNTNDYFSEITSLLNCMYEKGC
                     RFIEVIGEDILKNKSMREIFQYMLDHFDLIVVTKDSFTIDRSLIKELDNYRHKVIWKV
                     YSKNDDTRIKLKEDIKITSLIKRGHTVVRGDREKAIKIEDMRFKDTKERFSKYGAEWS
                     HLYISSDGSVKSYSFQKDKRIYLGNIFNNSVEEIFLEMQNKIDKLEIVYQ"
     gene            complement(51795..52667)
                     /locus_tag="PC471_000329"
     CDS             complement(51795..52667)
                     /locus_tag="PC471_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HDF2787471.1"
                     /translation="MIIKVSMTVLVLILLYILYKYETQHIETTEYTIFNKKIPKEFDN
                     FKIVQVSDLHNKVFDKNNKVLIDKIESLNPDVIFITGDLVDGENKNFQVALDLIDNLV
                     EKYEVYHIIGNHEQKSLIKKYKHLYKDYFKELYSKKIINMENESVRIYRGNSHLNLYG
                     LIIPLECYPYLFANSKKLELSDNFVEDNLGKVNENEYNILLAHTPFFFEKYAKWGADL
                     VLAGHVHGGIIRIPFVGGVLSPNREFFPKYDWGKYEKDNSTMILNKGLGGSKVLIRLN
                     CKPEIVKITLKYEK"
     gene            complement(52831..53382)
                     /locus_tag="PC471_000330"
     CDS             complement(52831..53382)
                     /locus_tag="PC471_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF2787472.1"
                     /translation="MDNKFPNVGENLRLLRQEMGISLDKASKMTGVSKAMLGQIERGE
                     SSPTVSTLWKISSGFKINFTTLLNENTNTYEVIKKEEVEPIVEQKGNMKLYPIYPFSP
                     QRRFELFIIELEENCTHVSSTHSHVLEEYVLVIEGKLDLNVDGKTYILESGHSIRFDG
                     TLEHIYKNLNKGKTIFHNIEVYR"
     gene            complement(53402..54304)
                     /locus_tag="PC471_000331"
     CDS             complement(53402..54304)
                     /locus_tag="PC471_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HDF2787473.1"
                     /translation="MSKKGRVVKVQHFSVNDGDGIRTTIFLEGCKLKCKWCSNPDSWS
                     NIVKLGVMKDKCVSCNRCIDVCPQNISSLFDRAQINNKCDLCGECIKVCLKDAICIMT
                     EEMSVEEIVEEVEKDFIFFFESNGGITFSGGEPTLQIDFLRELVDIFYDKGINIAIET
                     CGYFDWNKVNDVFEKIDHIFVDIKSMDDNIHKEYTGVSNKIILDNICRLSKLNKSMVI
                     RVPIIYGVNDSEENIRNTALFVKQNVPGGKMELLPYHKFGIDKYKALGLEDYIYEFDE
                     ICNNHMLKLKEIVELTGVKIIEYK"
     gene            complement(54319..56691)
                     /locus_tag="PC471_000332"
     CDS             complement(54319..56691)
                     /locus_tag="PC471_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase/glycerol dehydratase
                     family glycyl radical enzyme"
                     /protein_id="HDF2787474.1"
                     /translation="MSQTNRIEAFRQEYINSKPMICCERARIFTESHKKTEGEAICIR
                     RAKAFLETCKELPIKIFENELIVGTAGKFRRTGILTPEFSWQWVDKEMDTFDKRTQDP
                     YVISKEQIEFIRKEIFPYWKGKSLEEVFLARIPEDTAKILVDTGIIDNDSKWRQAVGE
                     VTPDYQDILFVKGYKGIKEDADKKIKELDISVSENIEKIDFYKSVSIVAQGIMTLAQR
                     YSNLAKEMSKQETDEKRKLELIKISEICMNVPANPPTNFYEAIQFVWFVQLGGILSEN
                     PLALNLGRFDQYMYPYYENDAREGKITESEAQELIEALWIKLSEWVWTISANTANYFA
                     GYNQFQNLTVGGKKRNGTDGTNDISYMCLKATESVKTHQPGLSVRVSQGAPDNFVMAV
                     AKLVKQGTGFPAIHSDSAGAQMLLQDGYDAEDARDWSNCGCVVPHFRKTGQWTSAVNI
                     NFAAALEYAMNEGKSRLTGEKMGLDTKNITEFTSFEELKDEFLKQLAYLVKSSVIGTT
                     VAQQIHKEMVPRPFLSTCVDGCLDKGVDLSKGGAKYNIGPVLTGIGLGVVSNSLAAIK
                     KLVFEDKVTTLEELTKALNNDWEGYEELRKLALDVPKYGNDNDYVDSLAIEVSDFYYT
                     ETRKYKDIFGSKFNSAFMGISNYVPTGKIVGATPCGRKATKPLTEGVSPFVGTDTTSP
                     LAAMKSASKINHDVHTGGTLLNLRLNQDLVETERGLRNLTSMIKSYFALGGFHVQFNT
                     ISNDTLLKAQENPEEYKDLLVRVAGYSTQFVNLSREMQDAIIARNSHSNF"
     gene            complement(56912..57601)
                     /locus_tag="PC471_000333"
     CDS             complement(56912..57601)
                     /locus_tag="PC471_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HDF2787475.1"
                     /translation="MKVKRNINRVAIIVALNLSLALMVGCMSPVSDKNLSSSEQISKD
                     SLKEQVEDKKDESIELFNNYSQKINDAKGREEVKKDLSQRFSKAIIDSGYSITNSKSD
                     GSFVVSINGAEKPPEKNIRSLFYSMSEDDKEGKVTIDILCAKEYPEDDKLSESDKYVK
                     FIYNLFKSLTDTNLTEKEIFSMVEEDFNKGVGVVELPYMKDIHVEVNKVKQSTKVLKL
                     SLVYKFDISNH"
     gene            complement(57842..59221)
                     /locus_tag="PC471_000334"
     CDS             complement(57842..59221)
                     /locus_tag="PC471_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HDF2787476.1"
                     /translation="MLNFMTELLVVLSIFIQFSIMNGILIYKKDSKKNEKIVFYIVLF
                     LIYIIIQISRLFNGGNYFSNINYLFYILAFIAWGGYYNYFYKMGYIKYSIFFYLFSVY
                     YKCLDAVIYRILFFAITGNVISSKESLLVPYLESIKIQIYSNLIIILIYLIFSFFRKS
                     IKLISQDKRNYVYLLFALLVNTMNMLVNYVLKKLERFGSLHSEGYYFDNFVNPKLVGA
                     SSIFLILLFKEIIKENRLKSQAELIKNKLDMQYAHYLSIQESHMRVKKLYHDINNHIY
                     CIDNLRNNSKEINEYVNNLKDEIKTFKYIYNTGNMILDIIINEKSEVCLKKGIKFTCS
                     INFSKVNFVKPIDVSSIFSNILDNAIEACDKIVDENINKYIRIKGTITRSFFVLKCEN
                     SKLNQLTFNKNILLTNKMDKFVHGIGIQSIKSSLQKYNGELLFENSIDKFILNIYIPL
                     DQDTDSWGD"
     gene            complement(59215..59928)
                     /gene="rgbR"
                     /locus_tag="PC471_000335"
     CDS             complement(59215..59928)
                     /gene="rgbR"
                     /locus_tag="PC471_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RgbR"
                     /protein_id="HDF2787477.1"
                     /translation="MIKIAVCEDEKETQLLIEDYLENILKDISIEYEIQKYISGEELL
                     ESNLKDIDILLLDIKMEKLNGMDTARKIREVDNEMEIIFVTSLIDYVQEGYEVRAYRY
                     LLKPIELEELKKHVLTCIKDIEINKESHITIKNKSNTYKIYLNEIKYIEVQKKDMLIH
                     TINKNFDIKYSLGKIEKELNPYKFIRCHKSFIVNLRYVENIKPNTAILESGEEVPISR
                     YRYKEVKEKFLKFLGDTIC"
     gene            complement(60178..60738)
                     /locus_tag="PC471_000336"
     CDS             complement(60178..60738)
                     /locus_tag="PC471_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2812 domain-containing protein"
                     /protein_id="HDF2787478.1"
                     /translation="MSAMEKKIKFKFDWYSSPDKIEKWLEDIEAKGYNLYKINKFGGI
                     FYFHKGSPRKIKYTVDYYYDVENNYFDEHKSNGWNLVFRSIGSFMGTSAWYIWSKEYK
                     DIPPKLYSDSDKTNKVKSILFMNMFIFFMLVVIFIYLLNIEIRNLYSGYGASVTAIVL
                     QIILLILYAVQLYRRLMYYLRLKKFK"
     gene            complement(60833..61636)
                     /gene="zupT"
                     /locus_tag="PC471_000337"
     CDS             complement(60833..61636)
                     /gene="zupT"
                     /locus_tag="PC471_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HDF2787479.1"
                     /translation="MNNVIFAFLITLLAGLSTGIGSCIAFFAKKTNTKFLSISLGFSA
                     GVMIYVSMIEIFPKAQDALTKSMGEKLGSWSTVIAFFIGMAIIAVIDKLIPQEENPHE
                     IKKMENIEEKNIKKNKSLLRTGIFTAMAIAIHNFPEGLATFISALDDVTIAIPIAIAI
                     AIHNIPEGISVSVPVYYATGDKKKAFYYSFLSGMSEPLGAIIGYVLLRNFLNDITLGI
                     VFAIVGGIMVFISLDELLPSAREYGEHHLSIYGLIAGMGVMAISLLLFK"
     gene            complement(61787..62950)
                     /locus_tag="PC471_000338"
     CDS             complement(61787..62950)
                     /locus_tag="PC471_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HDF2787480.1"
                     /translation="MDIKEITKSYKDYVIKLRREFHENPEKSMEEVRTSKRVKEELDK
                     IGIPYVSAGGTGVIATIKGDNPGKTVALRGDMDALQVVECTDVEYKSKNEGLMHACGH
                     DGHTSMLLGAAKVLNDIKDSINGTVKLFFQPGEEVGKGARAMIQDGAMEGVDSVFGIH
                     LWTDVESGTISVEEGPRMASADFFKITVKGRGGHGSLPHQGVDAVLASSAIVMNLQSM
                     VSREVSPLEPLVVSVGVLNSGTRFNVIASEAILEGTIRLFNPELRKQIPGILERIAKS
                     TAEAYRADAELEYGYLTPAVINDKECSKIATDAAIKLFGEDCITLFEKVTGAEDLAEF
                     MNIAPGALAFVGARNESKGACYPHHHGCFNIDEDALEIGTALYVQYAVDFLNK"
     gene            complement(63010..64197)
                     /locus_tag="PC471_000339"
     CDS             complement(63010..64197)
                     /locus_tag="PC471_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2787481.1"
                     /translation="MEAITAFCLVLVSLTIGDIVSAKTKAFVPSVFVSAIIFIVGFWT
                     FFPENIVDLAALGTPLAQLGMLLLITHMGTMMSIKELAGQWKTIVIALAGIVGICVGA
                     LALGTVVFGWDTAVIATPPLTGGLVASIMMADAATAKGLASLSVLAILMYVAQGFAGY
                     PITALMLKKEGKRLLSDFRSGKVTVNTDEEKVKDLPEQKSRFKIIPDLPEKYDTTYMI
                     ILRLGFVAWLAVQFTNLTNEVVSRYVICLIFGVIASELGIVDRKPLNKSGSFGWLMTS
                     LMAFIFAGLAKATPAMLVEMAVPLVGIIIFGVIGMGIFSIIVGKLLGESKEMAFSVSL
                     TSLYGFPPNYILTEEAVKALAETPEEKEFLMDKMLPKMLVGGFTTVTIASVVIAGIFV
                     NLI"
     gene            complement(64560..66260)
                     /locus_tag="PC471_000340"
     CDS             complement(64560..66260)
                     /locus_tag="PC471_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2787482.1"
                     /translation="MIKQFVKYYKPYKKIFTLDLIAAFLFSLCDLVYPMITRNIMDDV
                     VPNKNLRMLVVFAVALILIFIAKAGLNYFMQYWGHVIGVDMQADMRNEVFTHLQRLPN
                     TYFDNNKSGVTMSRIVNDLMDITELAHHGPEDLFISIVMLVGSFFILIDINIPLTLII
                     FAILPFIIWFAIAKKDKMNIAFMKSRVTIGDVNATLENSIAGMKVTKSFCTEKEELNK
                     FVRSNKLFRRARQDSYKVMAEYYSGMNLYMDILEWVVVIAGGYFTYIGKITLGDFAAY
                     ILYVKMFIQPMKKLINFTEQYQNGMTGFKRFIEIMEQDHQKEAKNPIELENVKGDIEI
                     ENISFTYEDKTQVLDNLSLSIKAGKTIALVGPSGGGKTTLCNLLPRFYEFDKGDIKID
                     GKSIKDVSLKSLRKNIGIVQQDVFLFTGTIRDNILCGNPNATDEEMIAAAKKARIHDF
                     VETLPDGYDTYIGERGVKLSGGQKQRISISRIFLKNPPIIILDEATSALDNVTEREIQ
                     ESLEELSKDRTNLVVAHRLTTIKNADEIIVLTDKGIEERGTHEELVNKNGVYSRLHNN
                     "
     gene            complement(66503..66778)
                     /locus_tag="PC471_000341"
     CDS             complement(66503..66778)
                     /locus_tag="PC471_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDF2787483.1"
                     /translation="MKKILVACGAGIATSTIVCDRVERLVKENNIQAEVEQCKIAECS
                     TKQEGADLIVSTTILPTTYDIPTIKATGYITGINTTALDKKILNALK"
     gene            complement(66897..67469)
                     /gene="mobB"
                     /locus_tag="PC471_000342"
     CDS             complement(66897..67469)
                     /gene="mobB"
                     /locus_tag="PC471_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HDF2787484.1"
                     /translation="MLLLDSFMKGHNFMYNILSVVSYSGVGKTTLIEGIIKELNKKGY
                     RVGVIKHTCHDFDMDEEGKDTYKHRKSGARKVCIISDKRVAYIEELKEKNPLYKMIQL
                     YEDMDLIIIEGYKNYRFKRLEVTRKGKYEDILSNKSDLIGIVSDIEYKLNIEQFNLND
                     YKNISKYIESCIKNEILSISNVEIKNILKE"
     gene            complement(67453..68634)
                     /locus_tag="PC471_000343"
     CDS             complement(67453..68634)
                     /locus_tag="PC471_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HDF2787485.1"
                     /translation="MIELEDAIKIIEKNVLPINRVKIVNLIDAVNKVVAEDIYSTIDS
                     PPFDRSPYDGYAYNANSENRNLKVIGTLYAGDVFNRVLNKNEAVKIMTGGKIPQGANC
                     VIKKEDVTVISDMITLNIKLKEYDNYIFKGEDIKKGQLIINKNQNIGYDGIGVLASLG
                     ISKVTVYDDLKVGILNTGTELQDINETLENGKIYDSNRYTLYSRLLKLGIQADTICNC
                     SDDILELEKKVKNMLQSVDFLITTGGVSVGDKDLIPDVFKKIGATELFWKINIKPGGA
                     CYVGTLGEKLLFGLSGNPHAAIVVFDNVIVPVIEYLTYKKGTHHIKAIFKGEFNKLSN
                     KDRFVSGKLYTSEGKLYVELNDKKDAKARLSATLRSNCMIKIKKNETIKENQLVDVVI
                     R"
     gene            complement(68845..69579)
                     /locus_tag="PC471_000344"
     CDS             complement(68845..69579)
                     /locus_tag="PC471_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2787486.1"
                     /translation="MKLKRSLVCVTILGIILVGCHKENTKEKNQVASKATQQKTMTKV
                     QNDVNEIMNKDYKYIIKNMGIPYNTFYYIKPKVLKESNTMQDINTSSYMTLVYPKYTG
                     NDELDGSALYVDINENKVVNVETNSFSSQGISVIDAESSIVIEKSDHEKSAVSLENFR
                     HIDLGEYVGVEDSRINEIVGDANYDLTAYNHEGSKVVKSYRLKEDNKILKKEVLTISI
                     VDNKIKSIKTIESDKIVKIIKGTLLE"
BASE COUNT        21874 a        12882 c         7319 g        27565 t
ORIGIN      
        1 taaattaata tttctaatat attttccctg gatttaaaat atttttaggg tcaaaagcta
       61 actttatatt tttaattaac atcatataag catctgaatt tgattcatgt aagtactctt
      121 ttttagcata tcctatgcca tgttcaccag aaacttgacc actaagttct ctagattttt
      181 tatacatata gtcaaaagtt tcttttaatc ttattttcca tgtattatca tccataccat
      241 cttttaaaat atatatgtgt agatttccat ctcctgcatg accaaaactt tttattctta
      301 tttttaattt gtcttgaagc tcgtgagtgt atcttataaa ctctgctatc ttatctcttg
      361 gaacaacgac atcacattca tccatttgtg tcgtagatgc ctttatagct tctaaaaatg
      421 cacctctggc tgaccatata gaatcgtttc tttcttgtgt atctgatata aatacatcta
      481 atgcaccatt ttctaaacat aaattagcta ctttttcata ttctttttct atatcttcag
      541 tgctatttcc atcaaatgta agcaaaagat aagcatctga tgtattatct ggaaactttt
      601 tacctaaaaa ttcttctgct gccaatatca catctctttc cataaattct atagccgttg
      661 gtattgattt tgattttatt attttaggaa cagtttctat tgccattgat aaatctggaa
      721 atggtataag caaacttata cttttctttg gtaaaggaag caattttaat atagctttag
      781 taactattcc taaagtacct tctgagccca ctaataaatc ctttatacta tatccagaac
      841 tatttttaac cacctttcca cctacattta taatttctcc atttggtagt acaacttcaa
      901 gtcctcttac ataatccctt gtaacgccat attttactgc tctcatacca cctgcattag
      961 tgtttatatt acctgcaatt gtagcacttt tttcacctgg gtcaggtggg taaaataaat
     1021 ctctatcttg aacatattga cctattgtca tcaataaaac acctggttct actgtcaatg
     1081 ttaaattttc ataatcaact tcaagtattt tattcatttt acatagatta atcattattc
     1141 caccatttat ggcaacagca gcacctacaa gaccagtacc ttgacctctt ggtgtgactg
     1201 gtatattatt tttatatgcg tatttcatta ttttagatac ttgttcagtt tcaagtacat
     1261 ttactaaaac ttcagggtac ttctcaatcc ctccaagttc atcatgtgaa aaatcttcat
     1321 ttatatcact tccaactaat acattttctt ctccacaagt atctattaaa aaatctatat
     1381 cttttttatc gattaattta tacatttcta tccccctatt tagctgctga tgccatatat
     1441 ttttgattat tatctttgtt attttctatt ttttcaatca atttaggaat aatatcataa
     1501 atatcaccta ttaaagctaa atgtgctaca tcaagtatag gcgctttatc atctttattt
     1561 attgctacta tatagtcagc accctgcatc cctgcaacaa attgaactgc tccagataca
     1621 ccacaagtta ttatcaattt aggttttaca gttctaccac taagacctat ttgtttttta
     1681 gcatctatcc aacctgcttc tattacaggt cttgttcctg ccacttgagc gtttaactta
     1741 tcagctaatt tatatatcat ctccatatct tcttgttttt ttattgctct acttgccaca
     1801 actatcactt cagcttcttc cagaccaact tctttatttt tttcttttac actcagtacc
     1861 ttaattttag attttagttc ttttttttct actttacata aagttatctt tcctgtagta
     1921 ttctctattt tttctggtgc tgaaaatatt ttatatctta cagtggcaaa ttgtggtcta
     1981 ttattaggtg tgtttatatg agccattata tttccaccaa atgctggtct tatttggtct
     2041 aaatcagtat ttgattgaat atcaagtatt gtacaatctg ctgtaagacc tgttctaaat
     2101 ctagcggcaa gtcttggagc taatgaccta cctaatgtag ttccaccaac taatactatc
     2161 gttggtttta ttttatctat aaagttttct attgcttttg aatatggttc tatcctaaaa
     2221 tctttaaatt catcttcatc atatacaaat acttcatcca ccccataaga aatcaaatta
     2281 ctgcacttat cttttatgtc ttttcctaca aatacacaat acacaggttg ttttattttt
     2341 gatgccatct cacgagcttt acctataagc tcgtacgtta caggatgtat atttccatta
     2401 tgatgttcaa catatactgc aattcccctc cattcatctt tattaatttg tactttacta
     2461 ctctctataa attcaaatgc gccttttggt ccacttttga cacacaactt gcacatctta
     2521 cagccagcac ttatttctac tatacctgac ttttcttcta tagccccaaa aggacatatt
     2581 tctaaaattc tctttaagtc agttatttta tcttgattta caactatctt tgacatttat
     2641 tactccccct tacaccaact ttaattcctc taatttattt acaagtgcaa aacttagttc
     2701 atctgcattt cctcttacaa tatttttatc tgtatttggc tttggaggaa atattcttac
     2761 aacttgagta ggagaaccat taagtccata gttattttca tctttatctt caaagtcatc
     2821 taagctatat acaggtattt ctctatcttt agtattaagt tttaatttaa atgaaggtag
     2881 tcttggttga aaaatgtctt tttctacagt tattaaacat ggatattcaa tctcacatac
     2941 ttgtaaatca tttggcatat ctatttcaac aactatactt tcatctttaa cttcaattaa
     3001 ttttgtaacg ttagtaacat gaggtatatc taaaaattca gctacttcag gtcccacctg
     3061 tgctgtatca ccatcagtag tttgttttcc acaaattatt aggtctacct ttcccatctt
     3121 cttaatacct tgtgatatgg tataggaagt agccaataca tctgctcctc caaatctcct
     3181 atcacttatt aatgctcctt catcagctcc catcataaaa ctttcttcaa ttaccttctt
     3241 tgcctgagga ggacccatgc taagtacttt tagattcgct ttcttatctt cctttattct
     3301 aagagctgtt tctaaagcat ataggtcata gggattcatt tttgaatcta ctccatctct
     3361 ttttagtact cctgtagttt catcaacttc tactgaagtc gttccaggaa cttgtttaat
     3421 acatgtcaaa atttccataa agtacactcc ttttttattt aactataact gatactccat
     3481 caggtataac tactactttg ctctccttac caactatact attcgccatt tctaaagctt
     3541 ctttaaagtt aaatgcatgt ttcatatgca ttttttttat catttcagga ttacacttat
     3601 ctgttaccat tatcacagta tatttagata atattctcgc tagtatctga aattcccatt
     3661 ggtctggaat cgtatctact ctatcgacac ttctaatttt atctaaaagt tgttttggag
     3721 aatctgcatt tgctacattt tcataaaaag attgacctcc atgaccatca ttacatgctg
     3781 aaatcattat tattactcca ccgtctttac aagtagcttc agctgctgtc attcctttaa
     3841 cagattgata aatattttgg tctagtggat atccaccatt tgtagttaca acaatatcag
     3901 attttataga atttactttt gataactctg ttacaaatct acatccttta gtatgtgctt
     3961 tttcgacatc accagcaaag gaagcaatta tttttttatc tttatctatt acaacattca
     4021 atatgaatgc taattttgca actttagctg catataccat atcctcatga ataggattat
     4081 tattcaattt tccagttctt gaattatcac tatctataaa gtcactacaa tgattgtaca
     4141 ttatagtctt tgcagatgcg attcctggaa gtacactttt tctgccacct gaaaaacctg
     4201 caaagaaatg aggttctata aaaccttctg ctatcaaaag ttctgcctca tatgctagtt
     4261 tgtttatata taaatctcca cctgaaggca atatccctac tttacacata gatttatcat
     4321 cagtagatat atgcataact atatcttcat tatctactat ttcttctccc attttataaa
     4381 ttagctcttc ccttgtactt ggcctatgaa atccagttgc cactataatc ttcacatcaa
     4441 tttctggatt aacattccta atccttctta atataattgg catagttatt ctacttggta
     4501 caggtcttgt atggtcactt gtaattatga ctacattctt tttcccctta accagttctt
     4561 ccagagaatt agagcctata ggattattca tagattcttc aactatctct tcttgagtct
     4621 tatgttctat ataattatcc gattttgact ccaaaacacc gagaaaacgt tcatcaggta
     4681 tttctaaaat cattccttgt tttgaatacg gtacttttaa ctttaccatt tttaatctcc
     4741 cattataata aactataaaa ttggtatgtg gtcataccag ttttacattt atatgatagt
     4801 accattatct accatttgca agaataattt attttataac aaacttttaa ttttcatttt
     4861 ttctataatt tttacttata aaatccatat gttcctttgc tttctcacat gcaagttcta
     4921 catcattttc tacaactgca ttgtatatag cttcatgctg tttaagcaat atattttcat
     4981 taaaaggttc tgcgattatt ttttctcttg catcttctat aaatccatca aataattttg
     5041 atgctgtcat aaaaagactt tgaattaaat aatttttact taatgttgct agtttactgt
     5101 gaaatttttg gtctaataac accagtactc gattattatc ttttttatta gtttctaccc
     5161 tcatttcatc tattatatgt ttcaattcta taacttcaga agaagttatt ctctttgaag
     5221 caagttttgc aatctcaatt tctaggatta ttctaagctc taatatatct tcaagtgtac
     5281 cattattaag tttaaaaatc atagataaag gttctattaa agttttttct atatttgtac
     5341 atataaaatt tccttcaccc tgtctgcttt ctatcactcc catagtctct aataccctaa
     5401 tagcttccct tatggaagtt ctactcacat tcatttgttc agacagttct ctttctgata
     5461 gtaatttatc cccctttttt aactctccat ttagtatttt atattgtatt tgctcaatta
     5521 cttgttcata aactttttta ctagctatac tattaaacat tttttcctcc aatatgataa
     5581 tcgttgttgt aatatatatc tattatatat cttaagccat tacattcata tatttttatt
     5641 acttttaata taatatctta taactcaaat ttacaggtga tattatgaaa aaaaataaaa
     5701 ttattgcttt ttttgctcca tctatcatag ttggagttct agttttaggg attatcctat
     5761 acccaaatga agctataaaa tctgcaagag aaggcttttc tatatggtct aatgttttag
     5821 ttccatcact actaccattt ataattggag caaacttaat tgttgattta aaaatagtgg
     5881 atattatagg gtttatcatc aatcccataa ctagatttgt atttaatgtt tcaggaaaga
     5941 gcgctttagc ttttgctata tctacagtat ctggttatcc agtaggagta agtttagctt
     6001 ctgaatttag gagtaatgga caaatttcta agtatgaagc acaaagactt gtgtcttttt
     6061 gttctacatc aggaccttta ttcataatag gttcggttgg tacaggtatg ttccaaaatt
     6121 caaacttagg ttatttaatg attctctgcc attatctagg aactatattg gttggtcttt
     6181 tctttagaaa ttatggtaat gaaaatcttc caaagacaaa attggattta aaaacaaata
     6241 taaaaaatgt aatcaacatt agaaattcaa gtgggaatgg tttttttgtt ttatttggaa
     6301 atgctgtatt taatggagta aatacattac ttacagttgg tggatttgta attgtttttt
     6361 cagtagtatt taaaatacta tctcttttta atgtaatctc attaatagca tcagttatat
     6421 atttgcctct ttcattttta ggtgttagta aagaactctg tcaagcattt gtaagtggtc
     6481 tatttgaaat tactatagga tgcaataaag tatctagtat aacaagttct ccagaaattt
     6541 taagagcatc tttagctagt tttttgatag gatttagtgg attatccatc ctagctcaat
     6601 gttgtacatt tctatcaaaa acggacatag atttaaaatt atacatacta agtaaatttg
     6661 cgcatggagt tttagctgct atctttatat ttatacttta tccaattgca aactctgctg
     6721 tgttggtttc tagctttgct gatacataca acgttatata taataattta atctggtttt
     6781 attatcttag ctattatgat acaattcttc aaattgtagt aataatttat ctaatctcag
     6841 ccgtatttat tgcaaaaaaa catatgaaaa ctttatctac aaatgggaaa ctaatgcgat
     6901 ataaaaatac ttttttcact aagaaatttt cacgaaaaaa ataaaactaa aaataaaact
     6961 aataaaactt aactaaatac ttccattttt ttattatatt tgtcataatt ttaataacct
     7021 ttttaattag tttaacagca tagttatttc atcacaattt aataatttta ttagaaatat
     7081 ttttcaaaaa attctcctta atcacatata tcacacttgt ataagtttac tgggaaattt
     7141 ttccattata taatatttca tttatattat ataataaata ccgacctaaa cttaccaaat
     7201 caaactaaga ggagtggttg aaaatggcaa caagacctat agaaatagac gaatataaaa
     7261 agataatgga actattacat actggcttta cttatagtga aaatggcgtt gaaaaaagat
     7321 ttagaaaaaa tcctaaagtt gcattggcac taatgttaga agctaattta ggtctaagaa
     7381 tatcagatat tttaagatta aagataggta attttaaagg taatatgttg gaaataaatg
     7441 aaaaaaagac gggcaaatta cagtatagac cagttaataa aaatataatt gagtccataa
     7501 atgaacatgc aagaaagtat aatcttaaat caaatgacta cttagtaaat ataaagacaa
     7561 aggctattca aaaacagttg aggataattt gtaaatactt aaatctgtat aatataagta
     7621 cacatagctt tagaaagctc tatgctacta ctcaatttga aaagagtaac aataatttag
     7681 agttggttaa agaactttta aatcacagct ctgtagccac tacacagcga tatataaggg
     7741 taactcagca agctattaat gaagctagtg aaaatttctt tattggttaa ttataacgta
     7801 tacatttttc tattatctta ttaattcacc agatattaaa aaagagtgtc tcaaaatgaa
     7861 ctttttagtt catgagacac tcttttttta taaaatcaaa tatattttta ttatttcaac
     7921 actggaaaaa aggcttatct ctctattttg agacacccta ttaaattttc ataattcttg
     7981 cataaacata ttaatctatc ttcaataatt ttttagctga ttcactgtaa ttatttcttt
     8041 ttacaatttc caaaactcta tcaatctcat gttctgactt tgcacttaca aaaatcgcca
     8101 attcttcatc atctataata tcataactat ctacatattt atctaaacat tctaaaattc
     8161 catctttatt attataccca ccaaatctaa ctactgcact acactcatct tctataagtc
     8221 cacttaaatc caatttccct tttgaaaaag actttatatt tgaacttcta ttatctatta
     8281 tatctataat atctgcataa acagcacttc ctgttgcacc tcttccagct cccttaccta
     8341 caaaagaaag ttcacctact gaatcaccta ctaaaataac tacgttgaac tcattatcta
     8401 tctttgaaag catattatta ttgtccacta atgctggtct aacaaatcca cttacttctc
     8461 ctgattcata cttacttcta gcaaccaatt ttatcttaca atttaatttt tttgcatatt
     8521 ctatatcttt ttcatctatt gaagttattc cttctaataa taaatctttc caatatacta
     8581 tttttccata agataaagta gacaatatag ataactttct agcactatca tatcctaaaa
     8641 catctgcatc tggattaact tctgcaaatc ccaactcttg tgcctgtttt aaagcgacat
     8701 catacggtaa attttcatca tacatctttg ataatataaa attacaagtc ccatttaaaa
     8761 tagcgtttat gctctttata ttgttgcctt ccagtgattc tatcaatggt tttaaaactg
     8821 gaataccacc tgccacagat gcttcaaact tgatgctaac attatttaag ttagctaatt
     8881 ctataaggtc actacctttt tcagccagta aatctttatt agctgtcact acatgtattt
     8941 ttttatttaa cgctcttgtt acataatcaa aagctggatt taatcctcca attacttcaa
     9001 ctagtatatc aatatccttg tcaaatatat cttctatatc agtagttatc ttatcaaaat
     9061 acttcttata cttgtgtttt tctttatttt taactaaaat tccaacaact tctatatctc
     9121 tttttgaagt attattttct attaaatcaa ttaatccact tcctactgtt ccatacccta
     9181 atattgctat cttcactgta acataccccc atcattttga ttatattaaa ttatttatat
     9241 atataaatat accaataaaa aaagcttgag tataaactca agccatatga cgggtttata
     9301 ctcatctttc agcataaatg ctgcaggatt tagcaccaat gtataattaa atatactggt
     9361 tgccgggctt cacagggcca gtccctctgc cactcttgat aagattgcta atttaaattt
     9421 atgtttaata gtataataca atttcttcca attgtcacta tttttttaac ttatttccct
     9481 aaaaggatat aaactattat ctaacagctc ttttagttgg tttacccttt tttgtaacat
     9541 tattattttt agtctttgct ttcgcctgtt catgtcctgg aaaaagtatt ttaggctctt
     9601 caatattcct cttgtaaata gtaaatttaa aaccaatagc ctgcacaaat tcagctctta
     9661 aagcttcaca tactgcatta gcagtttctt tagtatcaag acctgcattt tctaatatag
     9721 taacttttac taattctcta gttcttaaca taccatctaa ttgctctaag aaactctctg
     9781 taacaccttc tttaccaatt tgtgtagtag gctttaaagt atttgccaaa gatcttaaat
     9841 acgccctttg ttttcctttt aacatattct cacctctata attctatact taaattatct
     9901 ataatctata tttaaattaa ttgtaaaact caaattctaa ttcataaatt ttaaccacat
     9961 ctccatcttc tattcccatt tctctaagcc tatcaaaaac accttgactc tccatagctt
    10021 tttggaaata ttgaagggat tccatatcat caaaatttac agaatacatt attcttctaa
    10081 gtgctttacc tgtaactaca tacacaccat cttcaatatc tatacttaac tcttcttcag
    10141 taccaatatc aagttctggt ctatacattt cttcttctga aactagctcc acatcttcta
    10201 cttcttttag cattttagaa acataggcta ttacttcatc tatgccttga cgagttgctg
    10261 ctgacatttt aaatactgta tatcctctgc cttctaattc actcttaaac ttctcaaact
    10321 tactctcatc ttctaatata tcaaatttat ttgcaactac aacttgtggt ctctttgaaa
    10381 gcttttcatt gtacaattta agctcatcat ttatcttgtc aaaatcttca atagggtctc
    10441 ttccctctaa tccagatata tctacaatat gtatcaatac ctttgttctc tcaacatgtc
    10501 ttaagaactc atgacctaat cctatgcctt cagaagcacc ctctataatt ccaggtatat
    10561 ctgcaagtac aaaactatct ccaaacttag tttgaacaac acctagattt ggtgtaagtg
    10621 ttgtaaaatg atagtttgca attttgggtt tagctttagt tacaactgat agaaatgttg
    10681 atttacctac atttggaaat cctaaaagac caacatctgc tatcatttta agttcaagag
    10741 ttatccatct ttcttctcca tctgtaccag attttgcaaa agctggagct tgtcttactg
    10801 catttgcaaa gtgttggtta cctttaccac ctataccacc ttttgcaact atagctgtat
    10861 ctccttcttt ttttaaatct gctaatacaa gacctgtagc ctcatctctt attactgtac
    10921 ctgctggtac ttttaatact aaattttctc catttttacc agctcttttt cctttgcttc
    10981 cgtcgccacc attttgagct tgatattttt tctggtactt aaagtccatt agagttctaa
    11041 gccccaaatc aacttcaaat atgatgcttg ctccacgacc accatctcca ccgtctggac
    11101 caccagctgg aacgtatttt tctcttctaa atgctaccga accatttcca ccatttccag
    11161 ccttaacaaa tattcttgcc ttatctataa acaaattctt cacctactta attaaaaact
    11221 tttaattagc attaccaaaa ataccattat aaattcatca gtattataag aaaaaggagt
    11281 gcctcaacac tccttttatg tatattactc agctattgct actggatata tactaacttt
    11341 ttttctcttc ttgtcttttc tttcgaattt aacagtacca tcaactaatg caaataaagt
    11401 atcatcacta ccttttccaa cattagttcc tggatgtatt ttagttcctc tttgtctaac
    11461 tattatactt ccagcagtta ctaattgacc atcgaatctc ttaacaccta atcttttaga
    11521 tatagaatct ctaccatttt tagaagaacc tacccctttt ttagatgcta ataattgtaa
    11581 attcatgttt aacatatgga gtcaccccct acttttttaa aatttttata ttttttgggt
    11641 attcccgttt aatttcttta accgctaatt gaaagtgatt tagtaataat tgtgcgtctt
    11701 ttacatgtgt atctttgact atacactcta tatgaccttc ttctcctact actttttcaa
    11761 attcaatctt ctgtaatttg tctaaagaat ttataactgc aatagcatta gatgtaactg
    11821 cagcacatac tatatcatag cctatttcag caaaatcagc atgtcccttt aaacaaaaac
    11881 cttgcaacaa gaaatcatca ttataataat atttaacttt tatcataact taaccaataa
    11941 ctaagcgttt attttttcta caactatctt agtgtatgat tgacgatgtc cttgttttct
    12001 tctgtaatct ttttttgctt tatacttgaa aactataact ttcttagctt taccttgttc
    12061 aacaacttta gcttgaacag atgctccttc tactactgga gaacctaatt ttaattctcc
    12121 atctttagag caagctaata cttcgtttaa agtaacaaca tcacctgcgt tagcttctaa
    12181 tttttcaacg aataatacat caccttcaga aactttatat tgctttccac cagtctttac
    12241 tatagcgtac attaaataca cctcctcggc ctcaaactcg ccagtacaag gtacttcatt
    12301 tgaagattta aacctttact gtgcggcatt acaattatta attataaaat atttacattt
    12361 tattgtcaac tattttatcg aaaataacat tggttttatt gtgctttaat gtactatttg
    12421 cgtttaaaga aatctttata ttatattttt caccaatttt atcaattata tccatataat
    12481 attcttttat ttgttcaaaa ataaaatcat taaattctac cattatgttt ttgtaaacag
    12541 tatgttcact tattctcata atttcttttt caatactatc tagtatataa tgtacagatt
    12601 ttattgtttg ttcaccatca caacatggac aagacattaa ataatactta tcaatggatt
    12661 ctttttctct acgcctagca acttctacta aaccaagttt tgtcatacct aatacttcag
    12721 cttttctttt atctttatta aattcttctt ctaggatgtg taaaatctcc ttttgatgct
    12781 tctttttatg catatcaata aaatcgatta ttattatccc agctatgtct ctaattctaa
    12841 gctgtcttac aatttctttt gcagcttcaa gattagtttt ataaacggtt tcatcaagtt
    12901 taccagttcc aataaactta cccgtattta catctataac cgtaagagct tctgtttttt
    12961 caattattaa ataacctcca ctctttaacc atactttttt atttaataat ttttctattt
    13021 tagattctat tctgtataaa tcaaatacat ctctattttc ctcaagacac agtttatcaa
    13081 cataagtctt atctatacct cttaaaatag acttaagttc agagtactta tcataactat
    13141 tggtaattat agattcaata tcatcattca cattatcttt tacatattta gttgcaaaat
    13201 ctaaagactt atataataat ttcggaccta taccaagttt atattctttt aaaatgtttt
    13261 catactttaa tttcaactct tctatatctt tctttatttc atcatgagtg caaccttgag
    13321 cctcagtcct tattattaat cctagatttt ccttatttac tgccttagta atttttttta
    13381 atctaaatcg ttctttttca tcagtaattt tatttgatat tgtagttcta tcattggaag
    13441 gtatatagac aatatacctt ccagaaatac taatctcagt gtttaatttt gcgcctttag
    13501 ttcctatttc ttctttattt atctgaacca aaatatcctg accagatttt atagtacttc
    13561 ccttttttaa ttgtaaataa gcaagtttct taaatccaat atctacaaaa caagcttcta
    13621 ttcctggtat aacttttttt acgattccac gatatatatt ggatacagtc ttcttattta
    13681 aattgtcttc aacaaaaagt tctgttaatc tttcatcttc aagtactgct gttttctggg
    13741 accctattag ggactcaata actatctttt tcacaacttc acctctcgag cttacatagg
    13801 agtcactaat tccccatctt ctaatacata taaatctctt ctcaatatat caacatctaa
    13861 tgggtcaata tcaagttcaa acatctctaa tatctttggt ataagtatat tagtatttaa
    13921 attagttttt gagcctgtag ctatagtagc agtaagagta atatgttcat cttctaaatt
    13981 taaaacatca aaggttctta tcataggtct tatatctact tcaaccatct tacctttttt
    14041 attctttttt gttatgatta tttctttatt attcataaag tcaattactt ttctttttac
    14101 aaactctttc gtcataggtc ttttcaaatc tatattaaac aaatattctc catagtctat
    14161 cacagaagaa agagaaggtg tttgtctatc aatttctttt gctttgataa aatctatccc
    14221 atctggaagt tgaattgata ctttatttaa aaattcctct actgataaat catcttcttc
    14281 tatttcaata tctacatact caccttgact ttctgcccct aaagccaaag cattgccata
    14341 actcatttta gggtgaggat taaatccttg agaatgtgat aagtttattt cagctcttct
    14401 aaacgctctt tgtaacaatc tctgaaggtc aagatgtgat atatatatca tgtcgccttc
    14461 ctttttaaat tttactctta ttatcttact catattagca aagccccctt cctatatcat
    14521 gtatatttat tccacaacca ttgcagttat gtctacaatc agaagtgatt ttttcttttt
    14581 tagcattttc gtcttctcta attaagaatt ttttactaat acctacatct atatggtccc
    14641 aaggaaatac ctcttcatag tttctatttc tatgtacata aaaatctata gaaagattat
    14701 tcttttccat agcttctttc catatatcta aattaaaatg ttctgcccat ccatcaaatt
    14761 tagcacctag tctaaatgca tcatatataa cttttcctat tcttctatcg cctctagcta
    14821 ctactgcttc catcaaacta gtctttgaat catgataatt gtattttata ttgttatttc
    14881 ttaatttatt tactaaatgt ctctgcttat taataacttc ttctgtagta tcttgaccat
    14941 gccattggaa aggtgtaaat ggctttggta caaatgtaga agtactcact gtaacactaa
    15001 aacttctctt tagctttcct ccatttactt ttctataaat atcaataact ttataagcca
    15061 atttagctat accatctaaa tcttcataag tctcagttgg taaaccaatc ataaaataca
    15121 acttaacact attccacccc atctcaaaag ctctttctgt tgcattttcc aaatcttctt
    15181 ctgtaacacc tttatttata acatctctaa gtctttgagt tcctgcttct ggagcaaaag
    15241 taagtccaga ttttcttact tgttgtattt tatcagcaat ttccattgaa aaattatcta
    15301 atctaaggga tggaagcgaa atcccaatat tattagatgc atattcatct accaaataat
    15361 cagtcaactc tgataactta ctataatcac tcgtactaag tgatgataat gatatttcat
    15421 catatccagt attcttaact aatttatcta atatttcttt aagtctttta acactctttt
    15481 ctcttattgg cctgtatatc atacctgctt gacagaatct acatcctcta gtacatcctc
    15541 taaataattc tagtactatt ctatcatgaa cagtatctat ataaggtact attagttttt
    15601 ctggataatc tacagtctca acatctttta ttatcctttt agtaggattt ttagtaatcc
    15661 cttctctatt aggagttaca ctttgaactg taccatcttc attgtacttg acatcataaa
    15721 aacttggtat gtatactcct tctatgctag atattttata taaaaaatct tctctagtcg
    15781 ttttattttt cttccattct ttatactcat ttactacttc taaattaacc tcttcacctt
    15841 cacctaatat tactacatct acaaaatctg ctaatggttc ggaattataa gcacaaggac
    15901 caccaaccat tataaatggg tcttccaaag ttctttcttc ttttaatatt ggtatattgg
    15961 ctaaatctaa tatatttaag atattagtgt atgaaagttc atattgaagt gtaaaagtga
    16021 caaaatcaaa gtttgttata ggctctcttg actctaatgc aaataatggt atactttttt
    16081 ctctcattat attttccata tcaactgcag gagaatacac tctctcacaa aatacatctt
    16141 catccttatt tattacatca tataatatat gactccctaa gtgactcata ccaacttcat
    16201 ataaatctgg aaagcaatgt gcatatctta tcaattcact attactagta tctttatgaa
    16261 ttgaatttat ttcatttcca agatatctag caggcttttc tacttttttt aagatttttt
    16321 tcaagtctac tttattcatt tattaaaatt cctccaaaca agctctttat ttttattctt
    16381 atttataagt aatattatac acgataaaat attttctatc aaaaatataa ctaaaatatt
    16441 gaatttaaaa tacatattat tcacaaaata gaatagctaa aaatatttaa gaatattttc
    16501 tattaaattt ttattgaaat acaatttttt tacaaagtat gatatattat aaacataaaa
    16561 ctattgtttt acatgactta tcatgacatc ttttgtttca ttcacctcaa aatggggtat
    16621 gttacttata taatgataaa ttttaggagg gttaaaaatg agtaaagttt ttgaagtaaa
    16681 aaaagatatc tactttactg gagtagtaga tgaaggttta aaagtattcg acataataat
    16741 ggaaactgag tttgggacaa cttacaactc ttatttaatc aaggacgaaa aaacggtttt
    16801 atttgacact gtaaaggcaa attttaaaga tgagttttta agtaatttat cagaggttac
    16861 tgatatagct aaaattgact atgttgtaat tcatcacaca gaaccagacc atgcaggtag
    16921 tttaaaatac ttattagata taaatcctaa tatagaagtt tattgtacta aagctgctaa
    16981 actatactta gatggtcaaa taaatagacc ttttaactgt catgtaataa aagatggtga
    17041 aattttaaat atcggtaaaa gaaatcttag atttataact gctccattct tacattgggt
    17101 tgatacaatg tttacttaca ttgaggagga caagactctt ttaacttgtg atgcatttgg
    17161 ttgtcacttt gcaagtgttg atgcagaggt agtgaatagt gaagattatc taaaatcagc
    17221 taaacactac tatgattgta tagttaagcc atttgctaaa catgttttaa gtgctgtaga
    17281 taaggtggta ggactaaaca tagaatttga tactatattg acttctcatg gtccaatgct
    17341 tactaaagac ccaatggctg ctgttaaaag atatgttgag tggtctacag aagctgttaa
    17401 tacaactaat caaaatcaag tttctatatt ctacttatca gcttatagta atactttaga
    17461 gatggctaaa aagataaaag aaggacttga taaagaaggt gcaaaggctg aattatatga
    17521 tttagaagat atgacattaa cagaaatgca cgatacatta gtagtgtcaa aagtaatatt
    17581 actaggctct ccaactataa acaaaactat ggttaaacct atgtgggatt tattctctgt
    17641 tatagaccca atggctaacc aaggcaagat agcaggagta tttggttcat ttggttggag
    17701 tggtgaaggt ataactatgg ctgagacttt acttaaatct atgtcattca aaatgcctgt
    17761 agaatctctt aagaaaaaat tcttccctag tgaagagaca ttaaaagagt gtatggcatt
    17821 tggtgcagaa tttgcaaaat tagttaaata gccattaatc aaacataata ttatttaaaa
    17881 taaatgattt tatatttaaa aataaattaa aatggagttg tctcaaaatt aatttttcta
    17941 atctaatttt aagttcaact ccttttttat tttccaaaat gactaatata ataataaatg
    18001 taccctttcg cacagggtgc attatttata gtctgttcta tttgtatact agtatacttg
    18061 tattaattca cgtatctagt atatgctact agccctattc cagaattaga tgttgtttca
    18121 ccaatttcta ctttaataat tatattattt ttttacactt ttttgataat tctgctgcag
    18181 aaaggttctt accagtaaaa tctaaagaaa atgccttatc atactcaaaa ggataactaa
    18241 caggtttgct ctctggtgct aatgcaacca taccatatgg tgtattcata aaccctatta
    18301 cagtaactgt atgctttgat gggtctaaat ttgaattttt gcttgtaaac ttaattgtat
    18361 ttattgatga gttgattaaa ctacacggaa ctccctcata catttcaatt tttggacgta
    18421 gattatttcc ataaacttca tcaagtattg ccccttgttg atctgctatc atagtgtgat
    18481 tcccaggtcc aactggcgca gaaggcttag ctggcgtagg tttcttaaca gttggcgttg
    18541 gattctgtgt ctttactact tcgccaacag ctggatatgc tttcttgtca aatggtaatg
    18601 ggtgagtaag actcttgtat atgatgtctg ctcctgctga tttgttggca tcaatatcta
    18661 catgatacaa acgtccatcc tcaaaacgaa taagattcca ctgatggtca tttgttgtac
    18721 tatgtacata ataacattca attcccatag catcacataa agctttcaag ctcctacaca
    18781 ttccagaaca agcacctttt ttagtaacca atgtataagc agtattggct ccctctgctg
    18841 cgttccagtt aacatatgtt gtgttgtttt taatgtgatt atgtatagat acaatccact
    18901 atggagaaat ataaaagaaa agaatataac aaacaataaa aaataagatg attcaaattt
    18961 tattttgaaa catcttattt actctacaaa ttaaaattat ttggtgaatt atttaattat
    19021 tattttttat taaagaaatt ttaaatctta atataaaata cgctattata cataataatc
    19081 caaatacgaa tgctactgga attaacatct ccgattcaaa ataaaagaaa tattctaaga
    19141 aaatatctgg atttactata ttcccattct tgtctaaaaa atcttttcca ttatatccta
    19201 aatgctgtat aaaattactt atgttattat aatctgcttc cctctcatat accatattag
    19261 cattcaatcc agaagaactt aaattagtgt ttccatcttt catatctatt acatatagtc
    19321 taagagcttt gactttacta tacttatctt tatcaagtat tttttcaagt tctaacttat
    19381 attcaccaat ctttgtcata tcatgtcttt ctgccaattg ctttatttct ccatcaagtt
    19441 cttttcctaa attatacata ttatgttgta aactattatt gtaataccac ataaatccag
    19501 aaattaaaaa tgcaattata cttatgtaac ctaatacttt tttaaacccc attacaattg
    19561 atttatgcct atccaatgat aaagatagtt cttttattat attacataat tcatactgca
    19621 tttcatcacc atcatcaaat ctttcaattg cttttttaca tgcttcttct tcatctaatc
    19681 cttctaattt aaattcattt gcagattcta taagatggca tctcatttcc tgttttaatt
    19741 ctatagtact tttattgtca cattttttat atagtttatc taaatattca tctattagtt
    19801 tcataaatta atccacctct cccaaaaatt gattcataac ctttttaata aatctccatt
    19861 cttgtttctt tatttctaaa aaatctttac cttcattagt taaattataa tattttcttc
    19921 ttccacctga actttgttca ttgctataat aagatttaat taaattttta gattccattc
    19981 ttttaagtgc aagatacatt gttccttctt ttaattcaaa ctcacttctt tctcttactt
    20041 gctttgcaat ttcatatcca taacaatctt tcttttctaa tatagaaaca attaatatat
    20101 cgatataacc ttttaacact tctttattta actgcattat gtaccaccgc cttttcacct
    20161 actactctat aatgtatagt acttatattt actataatat tacatagtac tctataatac
    20221 aaggtttata ttctacaata gacttaaaaa taataaatag ctgtatatta atatttaatc
    20281 tataaatact aatatacagc tatttatcaa attttaaaaa taaatttttg ttttattaat
    20341 gttgtcataa caacatttac ttggttgaaa tagtattacc tataagttta aatatatata
    20401 ataatagact tgattctatt tcatattatt aaaatagagt aatagtttaa attctactac
    20461 tctatctata atctatttcc taattattta atctcaaatt tagctcttca tcatttaaat
    20521 ctttcataag atttctacag gtactagatt tattccaaca gaaaaactag ggcattaaac
    20581 acttatatat ctgtttcttt agactgtaat attaatattt tttaaatacg ttgacttttg
    20641 ctttttctaa cttctctcca tgatagttct cctctatcaa gagcttttat aaatatttct
    20701 gctgaagcta gatttgtagc tattggaaca tgataaacat cacaaagtct tattaacgct
    20761 tgtatatctg tttcatgagc ctgagatgtc aatgggtctc ttaaaaaaat aactaaatct
    20821 atttcttgtg ttactattaa agaacctatt tgctggtctc cacctaaagg accagatgct
    20881 aatctattta tatttaattc agtttcatcc attattcttt ttcctgtagt tccagttgca
    20941 tatagtccat acttttttaa aattgattca taacctatac aaaatccgac catagtgttt
    21001 ttcatttggt catgtgctac taatgctata ttcatatatc taatctcctc aatttatgtt
    21061 tagatgtatt taaatttaga aatttatttt ctttcttctc atacacacat ttagtactaa
    21121 tcctaagttt gctaaagatg tcaataacga acttcctcca taacttacaa aaggtaatgt
    21181 tactccagtt actggtatta acgcaaccgt catacctata ttttggatta tctggtaacc
    21241 aaacattccc ataacaccaa ccactatgag ggttccataa aaatcttttg catctcttgc
    21301 aattgctaaa agccttaata ggaatatcat aaataagatt atcaacactg ccattcctat
    21361 tacaccaagt tcttcaccta caacagcaaa tataaaatca ctatcttgta caggtaaaaa
    21421 atcttcttga ttttgacttc cattgtaaag tcctttacca gttacgcctc cagaaccaat
    21481 tgcaatcaga gattgcataa cttgatagtt tccttttaga gttacatctt ctggatttag
    21541 aaaagcttct attcttacct tttgatggtc tgccatcatt aaatacatca gtggcattga
    21601 cactaatagt atgattattc ctctttttat caactttgaa ctaagacctg ctgtgaacag
    21661 cattgcaaat atcatacaac aaaatacaat tcctgttccc aagtctggtt gtgcaatcaa
    21721 taatccgata aatggaagag aataaactac aacaggcata acttccttta acgtatttaa
    21781 tttatccttt ttggattcaa gtatctttgc ataacttagt acaaatgtta atttgactat
    21841 ttcagatgtc tgcaagtcaa gtggtcctaa gttaatccac gacctagctc caccttttac
    21901 tgtcccaatt ccaggtaata aaactatggc taacaaaata agacttatta tataaagcgc
    21961 tttataatac ctgcctagaa ggttatagtc aaaaaataat atgactataa tcattcctat
    22021 accaagtaca aaagctaatc cttgtttaat caattgatta tatgaacctg ttgaattagc
    22081 atgtgttgca ctactaagta taacaagccc aaaaataaag atagctaata ctgtaactat
    22141 aagtttccag tctaactgtt ttatcaactt aattgtcgac ttataattaa tattgatttt
    22201 tttcaaagaa tcaccgtctt tctatcttta aacatataat taaataaaag gggcatatac
    22261 caataaggat atcccccttt taagcactaa ttacatacat ctatctttta tattctttag
    22321 tggtatatta gcaactaatg cagatacagg tttgtcatca tgctcacctc tgcatttaga
    22381 catttttatt tcaagaccat catcttctat ttctgcataa ttagctatta ctttcaaaat
    22441 gtctgttttt atcatctcta aaagttgtgg tgaacaatct actctatcat gaactaaaac
    22501 tagctttaac ctctctttag caacagattt actagtttta gcctcgttag aaaaaactct
    22561 aaataaatct aacacagatg tatcctcctt tgctacttag ccatgctaaa cattttttta
    22621 agtctaccaa agaatccatc ttcaacttca agatcaagta gaggaacttc ttcatttagt
    22681 atcctttttg cgatattttt gtatgcttga ccagcaagtg acttagaatc aagtatagct
    22741 ggctctcctt tatttgttga tataattatg ctttcatcat caggaacaag acctaacaaa
    22801 tctattgcta atatttctat tatatcttgt ttatccatca tgtctccacg cttaaccata
    22861 tcatttctaa ttctatttat aactaatctt atctctttta tttcatttgc ttctaataga
    22921 cctattattc tatcagcatc tcttactgct gatatttctg gattagtaac tacaatagct
    22981 ctatctgccc cagctactgc atttttaaag ccttgctcaa tacctgctgg acaatctatt
    23041 attatatact cgaaagattc ttttagtttc tcacacaggt caatcatctg ctcaactgag
    23101 acagcatttt tatctctagt ttgtgctgct ggtaataaat atagattatc aaatcttttg
    23161 tcttttatta gagcttgctt aagcctacaa gttccttcaa ctacatctac tatgtcataa
    23221 actattcgat tttcaagacc cattactaca tctaagtttc taagtccaat atctgcatcc
    23281 acaactactg tttttttatt ttctaggctt agcgcagttc ctaagtttgc tgcggtagta
    23341 gtttttccaa ccccaccttt accagatgtt ataactataa cttcgctcat aatactaccc
    23401 cctatttttc tttctatttg tctaactttg acaaatagct ttcgattaca attcttccat
    23461 tacttacaaa ggctatttct gggctaattt ctgattcact ttcataattt tcttcatcat
    23521 ctggagcttc tgcaatatta tctgctattt gtaatatttt aggattaaga tttccagcaa
    23581 caacatatgc aaattcattg ccttctgcac ctgcatgtac aaaaccttct attttaccca
    23641 ttacaaaaac attacctcct gctacaacct tagcaccagc atttatatct cccataacaa
    23701 ctgtatttga ttttgaaagg acttttgaac caggtttcat atcattcaat ataactacat
    23761 caccttcaaa ttcaatattt tcaccagatc ttagggtatt gacatatttt gttggaaaat
    23821 tactattttc ttcaattttt tgatcttcaa caaattccac atcaaatcta gaagtaattc
    23881 cttctttaag ttctagaatc tccatgtcag ttaaacaatc actattaatt ttactaattt
    23941 tagccccatt aaaaaagcca acatatgctt ccaatttgtt tattattttt tcctgtattt
    24001 cctcaaatgg agcttctctt tttatattta ctattatccc tcttttatta cccttgaact
    24061 ctactagttc ttgactacaa atttctctta aagacataac aacctccaga gtgatatatc
    24121 atttatattc ttcaaatacg ctttaaaccc ttttattttt tctatataaa gctaaagttt
    24181 atacatagat tgagctatct attaatttgt gactcgaaat ttatattttc gtcagaagaa
    24241 ttgttaacat cattcgtctt gttgtcattt ttactattag ttggctctaa tcccatataa
    24301 gttccaataa cttctctagt tggtaaaagt gagaatacac ttgaatcacc ttgaggaatt
    24361 acacatacaa cagctatttc tgggtcatct gctggagcaa aagcaacagt ccacgtaaaa
    24421 gaaccataat cttgtttaaa tctatctatt tggtcatctg ttatttttgg atttaattct
    24481 tttatagctt ttcttagata tgaagaattc accttatcag tgtcctctaa tctaactttt
    24541 actccatctt ctaattcttc ctctaatttc tttctttctt cgtctttcaa atctttattt
    24601 tctaatttct tttttatttc cttttcctta gctaatgaaa gctctttttc tttttctgct
    24661 ttcatcttat cagctagttt aatcgcatca tttaggttga cattataaga tgacatatga
    24721 ctttttaaat attcatactc gttatctgta ggtattttcc cacttttctc agctgtacca
    24781 gtttttgctg ctacatctat tggaaaatca gcaaaagcac tcttcgctgt accttgtctt
    24841 gcaaccaatt tcataccttt tgttaattct tttaagttat ctggattttt aaaaggtatt
    24901 ttttcgactt tcttctggtc attaatttta actgatgaat aatcacttga aacagctcta
    24961 tcaactacag ataattctac taaattacct ccatttgcta tagcagctac atatcttgat
    25021 atttgagctg gtgtataagc attttcacct tgacctatag ctaagttaaa tgtatctgca
    25081 gtactccatt ttgcaaagtt aaaataacta aatactgcta aatctgctac ttcttcaatt
    25141 ctatcttctt taacatccaa tttttttagc ctattcattg tttcaactct accaggagtt
    25201 ttcttttctg ctgcccagct tacaatctca ttgattcttt tctcatattc tttaggattt
    25261 ttttctctag ttatatcaac aaaatcattt gccatttctc tttctaaatt tgactttaga
    25321 agagcttgtg ttgatttcaa tttatcttct gtgctaggaa ctttacctgc tctttcttct
    25381 acttcatctt gaagccctgt attttggtca agcccaaata attttgcata ctcaagtatt
    25441 ttatttgcat tcatatctat accaatgctt tttccatctg gccatgtttt acctgtacca
    25501 attgttgcca tatatatatt acaagactct tgtattgctt tatataagtt tgtcatacca
    25561 tggtttcctc taccatggtt ccatacatag tcaccaaaag attttttacc taacattata
    25621 accccagggt catttatagt aaagtttgga tccagaccat tttctaacgc tgccattgaa
    25681 gttaccatct taaatgaaga acctggctga aattcccctt gtgtaaccaa gtttaaaagt
    25741 ggacttcctg ctagtaagtc atttggattt tttggttgaa gtgctttata atcctctgta
    25801 gaaatacctt ttgcaaattt atttgggtca taattaggat aacttgatga agctaatact
    25861 tctccatttt taacatcaat agctataaga gctgctgact gtgcttttcc tgcatatgca
    25921 ctaattggtt tgtccccaaa tttactttta aaagtacctc ctttagaagc aacttcgatt
    25981 atctgtttta atgcattgtc tgaaacttct tgtaaatctt tatctagtgt aagatacaca
    26041 gtatctcctg atttaggttt tttagattca atttctttac tgattctacc caatgcatca
    26101 actttaacca ttttataacc atcagttcct cttaatctac tttcattact cttttctact
    26161 cctgcaagac ctaccatatc tccagtttca taaccttttt gaaggtatga ttctatttga
    26221 gtggatggca tttttcctac atatcctaac atatgagaag ctaatgaccc attaggataa
    26281 tatcttactg gttctactgc tattgaaaca ccaacaaggt ccatagcact ttcttcaatt
    26341 tgagcaatcg tagtttctcc tacatcctta gcaatagtta ctggattata ttgagaatat
    26401 ccttttgatt ttatcaaatc ccttacgacc attatttttc ttgcatcttc atcactcaaa
    26461 cttttatcaa tttctaatgc atcactattt cttacttttt caaacgcttc tttggcactc
    26521 agtttagttt cttttatctt ataactagcc aaccaatctc tcttatctct ttctgctgtg
    26581 aacatccatt tactaactaa aataggtgga taatatccat tttcatttag ttttgcctgt
    26641 aatcttgtcg catccaatct tttatcttct tgagataata ttccagctga tataagtttg
    26701 tccaccaagt aataaaaact ttccttagca ttataatcat ttggaatacc attttctgat
    26761 ttatattccc taatatctct gtcatatgta taatagtact ttccattttc tacatatata
    26821 ggaaactcat ctacatattc ttcaccattt ctctccagca aatttataag ttttaatgat
    26881 atttcatttg ctcttgaatg cttatttgca tctttcttat taatatcgtt acctgaaact
    26941 tgaacagtaa ataagttttt atttcctgcc aacagtcttc catatctatc ttttatttct
    27001 cctcttggag cttctattgc caacttttta tatgttttat tctcagctaa ctcattgtaa
    27061 tattcatatt taaatgtagt catatagatt atttttacta aaatagctaa aaatatcacg
    27121 cccatgacta tcttaattat ttttagtcta tcaacttgtt tattttcttc catgaaatca
    27181 ccaactaatc ttctttaagt tttaaaactg ctcttttaga taagcgatat attactaaac
    27241 caattaaact attcactatt ggtgctacta gtagaccttt tacaaaaagt gttgatatgt
    27301 tgtagctttg ataaattata ctagatgtac ctatattgac aattgaatca attaaactgc
    27361 ctataaaaat taaaacgaaa gtaataatat aactaccttt atatagctga tctctcatgt
    27421 atccaattgc atatgcaata ataaacaaaa ttaatgcatt tacaccaaaa attccaccaa
    27481 cggttatgtc tttaataagc cctattattg ctgctataat cccaccttct aattcatcta
    27541 aataaagaga gattatggtt acatatataa gaactaaatt aaagcttatg ccaaaaatat
    27601 ctatataatt tgttatacta ttttctatag tcataagcac aatccccaac agacaaagta
    27661 aaactttttt cattgttttt atccccttta tcctatattt cttggttcta tgactacaac
    27721 gtcatttata ttcttaaaat caacataagg ttttacaact acatatttta atgacttatc
    27781 tttatcatca accacttttt caacttcgcc aattggtata ccatctggga agaatccaag
    27841 acctgatgtt gttattatat ctccttgaat tacattatat gatgagtcaa acatatatcc
    27901 ttgcaaatat cctttactat tataactatc tttattatct aaagtagtat tttgagtaat
    27961 tgtaccttta gcattggcat cctttgctag tttaaagctt acagatgcct tagaatccaa
    28021 tagtgaaatt gccttactat aattgttgga aacttcataa actatgccaa ctaatccact
    28081 accattcata actatacttt ctttttttac acctgaattt tttcctgcac ctatgacaaa
    28141 actctcatac cagtttccat catttttacc aacaacactt gctgaaatac ttgtcgcctt
    28201 atactcttct tgtacaaagt tcagtgtctt ttttaactct tctagcgatt ctgttttatc
    28261 tagtttggca tttaaagcta tcatattttt tttaagtttt tcattttctt cttctaattt
    28321 ttttaccttt ttagaattat ctttaaaatt tgctacatct ttaaaaccat ttttgataaa
    28381 agaaaatcca tcatttattc ccttacctac acttgtaata gcatctgaag ctactcctaa
    28441 atttactgga ctaccactag aaaatttacc aattgatata cccacaattc caattaaagt
    28501 gatagcaact acgcccgttg ctatcacttt aacattaatc ctttttttct catttttgtt
    28561 cttgtcaaat ctcaaggcca tcaccaactt ctaattctta gtattcatca ttaatacttt
    28621 ttcaaagatt tcttggtctt caacagattt acctgttcca agtgcaacac aatcaagagg
    28681 tgtttctgct atttgtacag acataccagt ttcctgtgta ataagcttat caagacctct
    28741 aagcaaagcg cctcctcctg ttagcattat tccgttttcc attatatctg aggctaattc
    28801 tggtggagtt ctttctagtg tagattttat tgcatctact attgaactta caggttcttt
    28861 taatgcttct ctaacttctg tagagcatat ttctattgtt ttaggtaatc ctgagattaa
    28921 atctctacct cttatttgca tatttatttc ttcatcatct ttaaatgtag aacctatgtt
    28981 aatttttaca ttttcagcag ttctttcacc tatcataaga ttatattctt tttttacata
    29041 agccactata gactcgtcaa attcgtctcc acctattctt atagatttag cagtaacgat
    29101 tcctcctaaa gatattacag ctatttctga agtacctcca ccaatatcaa ctaccatatt
    29161 accttctggt tcttcaactt ttaatccagc ccctatagcc gctgccattg gctcttctat
    29221 aaggaatgca tctttagctc ctgcttgtct tgcagcctct tctatagctc tcttttctac
    29281 ttctgtaact ccaaaaggaa cacatatagc tattcttgga cttacaacac cttttttatc
    29341 agccgctttt tgtataaaat agctaatcat tgattgagtt atgtcaaaat cagctattac
    29401 tccatctttc ataggtctta tagctactat atttcctggt gttctaccta tcattttttt
    29461 ggcttcttca cctactgcta atacctcttt actatcatct cttattgcga caactgatgg
    29521 ttctctaaca acaatacctt gtccttttat gtaaactaat gtatttgcag tccctaaatc
    29581 tattcccata tcctttgtca tcttattaaa actaaaaaaa cctttttttt ctttcttttt
    29641 ttccttagcc atgattttac tccttcctac tattaagaat catgtttaaa acattcttct
    29701 attattttat cacatataaa gtcaaattat catattttct ttaaagctaa agtataatcc
    29761 atctccaata attatatggt ctattatttc tattccaatt atttctccac atttaataag
    29821 tcttgatgtg atatttttat cttctcttga tggttctaca cttcctgaag ggtgattatg
    29881 catcactatt atcttacttg cacttcttct tatagcttct ttaaaaacct ctcttggatg
    29941 gactaatgac gagcttaaag tccctataga tacatctaca tcagatatta tctcattttt
    30001 tgtgtttaaa agaaccgctt taaaaacctc tttttttaaa taccttaggc tatccatata
    30061 atacctctgt atatcccaag gattccttat tttatacttt ataggtttaa aactagctac
    30121 tctagagcct agttctaaag ctgctttaat ttgggttgat tttgataacc caattccatc
    30181 tatattacac agctcctcta ttgtcatatc ttctaaatgt ctaataccct gaatatctct
    30241 atttattata tagttggcta attcaatagc atttttattc ttgttgccag ttcttaaaag
    30301 tatagccaac aactcagcat ttgataagct ttttacgcct ttggcaagca tcttttctct
    30361 gggtctttcc tctggtgcca tttcttttac cttaatggtt gaattaaatg actttttcag
    30421 ataaatcaca ctccataaaa aagatttata ctaaaatatt tttttaatag gtcgcctaac
    30481 cttgaaattg gaagaccaac tatattaaaa taatctcctc taatttcttc aactaacaaa
    30541 gctccataac cttgtatacc ataggctcct gctttgtcaa atgactcatt agttttcaga
    30601 taatcctcaa tatcctgctc tgataacgtt ttaaatttca cattgctgat tacatagtca
    30661 attacctttt tatcttcgca caagtttatt aagctaatac ctgttatgac ttgatgctct
    30721 cttccagata gtctttttaa catatctctt gcacaagact catcttttgg ttttcctaaa
    30781 atagcattgt ctaaaacaac aactgtatca gctccaataa caacatcttc attatgttca
    30841 gatgctacag acatactttt ttcaaaagct aatctcataa ctaaatgttt tggtgtttca
    30901 ccttccaata taatctcatc aatttcattt ttaattatat caaatcttac attcgtattc
    30961 tctagaattt ctttccttct tggagaggct gatgccaaga ttatattcat ttattcacct
    31021 catttgtacg taatttgtac ctatttagtc tatcactata ttgtaaaaaa ttcaacagaa
    31081 atgtcgaacc ttttttgtta cactattgtt atatttatta ccattagaat aaatatacat
    31141 aaaatagagg tatggaaatt attccatacc tctgttttat gtactcatta gagatatgga
    31201 aattattcca tatccttatt ttgtccatat tacaaaattt taatagcttt ctttggacat
    31261 ttttccatac ataaatggca tcctacacat ttatctgcat ctaccttatg tttttctttt
    31321 aattctccct ctattgcatc aaacttacat tgttttttgc acactgtaca tccaacacaa
    31381 agttcttggt caatagtaac tttctttttc ttagttatgt cacctgatat tacttttgta
    31441 gggcattttg ctacacattg catacaacct acacacttat tagggtctat cttagctatt
    31501 ttgtcttcaa atattattgc atcaaattta caagctttta cacacattcc acagccaata
    31561 catccagcag tacatttttc tttaacagct ttaccaaact ctttgctatt acactctaca
    31621 actacttctt gactttctgg ttttgtgatt attataccct taggacaaac ttctttacat
    31681 tttccacaat ttacacactt ttcttcatct attactgcta ttccatcaac tatagaaata
    31741 gcatcaaatt tacaagcatc tttacatgtt ccaagaccta aacatccaaa tttacacatc
    31801 ttagcacctg agtttaaaac atttgctgct ctacaatctg atattccttc atactcatat
    31861 ttatcttttg cacttgaaca agttccttta caaataactt ttgctacttg tttctcacct
    31921 gcttcagcac ttattcccat tatttctcca acttttgcac caacatctgc tccacctaca
    31981 gggcaaccat taactggtgc attgccttca actatcgcat ttgcaagacc tccacaacct
    32041 ggaaatccac aacctccaca gttggctcct ggaagtactc ctagtatagc ttccactctc
    32101 tcgtctactt ctacagcaaa ctttttagat gcaaaatcta gtactatccc gaagattaag
    32161 cccataattc ctaaaactaa tacagctgta agtatcacca ttttcacccc tcttttttat
    32221 agctttataa gtcctgtaaa tcctaagaaa gctattgaca ttaaacctgc tgatataagc
    32281 gttataggga aacctttaaa tgcttctggt atatcagcta attctagtct ttctcttata
    32341 cctgcgaata ttactattgc taaagtaaat ccagctccgg ctccaaatcc gtttattatt
    32401 gtttctacta aattataact tttttgaaca ttaactagag ctataccaag aactgcacag
    32461 tttgtagtta taagaggtaa ataaactcct aatgcttgat ataaagatgg actcatcttt
    32521 tgaattacca tttcaacgaa ctgaactata gaagctataa ccaatataaa agctattgtt
    32581 tgtaagaatc cagttccagt ttttactaat aaaatctgta taaaataagt tataattgaa
    32641 gctagagtta aaacgaacgt aactgcaact cccatcccta ctgctgtatc aaccttctta
    32701 gaaactccca agaaaggaca tatacccaag aattgagatg ttataacgtt attaacaaga
    32761 acgatactta aaaataaaag tattaaattc atttattaca cctccactat gcttttttac
    32821 ttcttaattt attaaatcct gcaaataaga atcctaatgt taagaatgca cctggtggta
    32881 atataaatat taatactggt tggaaagatg ctccaaataa tgtcattcca aataaactac
    32941 catttccaag taattctctt actgcaccta ttattgtaag tgcaacagta aatccaagac
    33001 cttgacctac accatcaact attgaaggca tagctcctgt tttgaaagca aaactttctg
    33061 cacgagctaa tattaaacag ttaacaacta ttaatggtat atatatacca agagccttat
    33121 caagagcagg aacatatgct tttaaaagca tacctactat tgtaacaaat gtagctataa
    33181 ctactataaa tataggtatt cttatcttat ctggtgtaat ttttcttatt aaagatataa
    33241 ctaaatttgc acatattaaa actgctgctg ttgaaagacc cataccaatt ccatttattg
    33301 ctgaagttgt aacggctagt gttggacaca tacctataac ttgaacaaat gttgggttct
    33361 catctattag cccattttta aatactttag ctaaattcat aattaaccct ccttatttat
    33421 taagagcact attgaaaaca tctatagcag catttactcc tgtagtaact gctgtagatg
    33481 taatagttgc acctgatatt gctaatattt gattatctgc tgttgtactt ccttttgcta
    33541 cttcaagagg ctcagcagtt tttcctttgt attgaccctt aaatgcatca tccttagctt
    33601 ttgctccaag acctgctgtt tctgacatat taccaatgtc aacacctgta acttgacctt
    33661 cagatgatat acctaccata acttcaattt ctccaccata tccacttggt ttagctttta
    33721 aagtatatcc aaccacttca gaaccatcta aaccttcata cacttcagct ataagaccat
    33781 ctccaaggcc tttgtaagta tcttccttca tttctttaaa ttcttttgct tctggaagga
    33841 ctgcttttct aagttcatta tttgcttgta tatttctttg ttctattact ggagctgtaa
    33901 cctgattagt agctccaaga gctaatgcag ctattgcact tatagcaagt aaagtaccac
    33961 ctaatctcac catactgttc attatttagc cacctcccca aatactctat ttctaacaaa
    34021 cttatctata agtggagcaa gacagttcac taatagtatt gagtaagata caccttctgg
    34081 atatccacca tacattctta taacagttgt tataagacca gctaatacag cgtatattat
    34141 ttgacctttt ttagtcatag gcgaagttgt ataatccgta agcatgaaga atccacctaa
    34201 cataagtcct ccagcaaaca gttgatatat tgcgaaagtt atattaaatc cacctaaaag
    34261 gaatgttaat ataaatacag ttgctatgta aaatactggt ataacataat ttattatgcc
    34321 tttatacatt aggtatactc cacctaataa taataatatt gctgatgtct cacctataca
    34381 tccaccaata ttccctatca tcatgtcagc aagactaatt ccattgctag aaaggtctgc
    34441 cagtcctgct gctccatttt tcaaaaagct taatggagtt gctgtagtaa ctgcatctaa
    34501 atttttacct atccagttaa cacctggttg agtccaagca gtcattgcta ctgggaatga
    34561 tgctaataaa aatgctcttg cagcaagtgc tggattgaca aagttacatc caattccacc
    34621 aaataccatt ttaactacta atattgcaaa tattcctcct actagacaca tccaccaagg
    34681 aagtgatgct ggtacgttaa atgctaataa taatcctgtt acaacagctg aaaaatctcc
    34741 tatagtactt ttttgcttcg taaatttttg atataaaaat tcagtaccta ctgtaccaag
    34801 tatacaaaaa aacatagcac tcaaagcatt aagtctaaag aagtatactc ctgctactgc
    34861 tgctggaagg agtgctataa taacttgttt cattatatat gaagtatctt cattacttct
    34921 cacatgagga gaagatgata ctatcaactt attttccatc gtttttcccc cttaactttc
    34981 tttatttctg agcagcttgc tttcttctct ttgctcctat ttctctcttt gctgatctta
    35041 tagattgaag taatggtctt cttgctggac aaataaatga gcaagaccca cattctatac
    35101 aatccattgc tctatgatat tcagctgtgt catagtcatc ttttaaagaa tatgcactta
    35161 tgaaaagtgg ttgtaagaat gatggacata catctgtaca tcttccacat cttaaacagt
    35221 tttgaatatc tggagtacgt gattcttcct catccaaaca taaaattcct gaagaacctt
    35281 tgttagtcgc tatttcagta gtatattgag ctattcccat cataggtcca cccattataa
    35341 ccttaccaat cttcttatct tctttaaatc caccacattg ttctattatt tctgaaacta
    35401 atgttccaac ttttgttatt aggttctttg gctctttgat gcaactacct gttattgtag
    35461 tgattctttc aaccaaaggc atacctgttt taatagactt agctatctgt gctgctgtgg
    35521 caacgttgtc aacaactgca cctgctgcta ttggtaatcc accagatgga acttctttgc
    35581 ctgtacacgc ataaataagt tgtttctctg ctccttgtgg atatttcact tgaaggccaa
    35641 caacttcgat ttcgctatag tctttagcta cattttgaat tgcttctata gcatctggtt
    35701 tattcgtttc aattcctata aatccttttt tgacatctaa taccttcatt aatgctctta
    35761 aaccaaacac aacatcttct ggattttcaa ccattaatct gtggtctgca gttaagtatg
    35821 gttcacattc tgcaccattt aatataacta cttctgcctt gctatctggt ggaggagata
    35881 cttttacatg gttagggaat gtagctccac ccatacctac tattcctgcc tcttttatga
    35941 tacctactat atcttctttg ctcaaatcct ctaactgacc ttttggttga acactctcat
    36001 gtaattcttc cttaaagtca ttttcgataa ctacacattg agctgaacct cctggtactt
    36061 catgttgttc aatagcaata actttacctg atactgaaga atgaacgtta catgaaacaa
    36121 aaccttgttg ttcacctatt ttttgcccaa actttacttc atcaccaact tcaacaatag
    36181 gtttagctgg tgcccctata tgttgctgaa gaggaatgta aactatcttt ggagcttgag
    36241 ctttttcaag tgcctttgtg ttagagtact cttttctata tggaggatgt atccctccct
    36301 taaaagttaa gagtttcatt gttttacccc cttatctata aaaaaacatt ttttctgcct
    36361 tctataagta tactatatat attaaaaaat cttaacaatt gttaaaaaaa tatccgtatt
    36421 tttataattt attttataaa tcaacacctc tatattaagt caaattgagg aaaaggattg
    36481 cttaattaaa caatcctttt catatagctt tctaaagtcc aaattttctg tattttattt
    36541 tatagaattt ataaacatag agtactcctg catggatgat aaaagcagac ttttacttac
    36601 actatatttt tcttcatttg ctgcttttga agctgtatca attttttcat tcacattctt
    36661 tacatactct attaaattat ctttaaataa acgcatttca gaataatcaa tcttttcagc
    36721 ttcttttgat atattttgtg atattgcctt tctatttgtc aaaatagtat tatatttttc
    36781 catatctaca ttattttcag cattcgacca aaaacttgat tcttctttaa agtttgttat
    36841 taatttattt aattctttag atatactctc tatataagcg tagttacttg tatactctaa
    36901 agacaacata ggtgcatcta agtgtgataa aaatgcatct ggaaacacct ttcttacact
    36961 gcttatctgc tttctaacat cattttcatc aaaagaggaa taggtctgta ttttttttac
    37021 tccatccttc tccataactg aaaaaggtat gtcattatta actaatgatg tctgtatttt
    37081 tttcaaatca tcattattat ctattgcagc aacttgaata gtatacatat cccagccttc
    37141 tatagtagct aatttgactt cttctggctc tttagtgtta tttaccgctt tttctggatt
    37201 agtttcttgt ttatcatctg aattggtttg tgatttagat tttttccctt tttttattga
    37261 atttaattca tcagatatat ctttataagt tatatctgat gaattattta gaaatggtat
    37321 tttagccgat acatactcca atatcttgct atcttttatt tttgtataag aataatagcc
    37381 aattagacac aagcaagcaa tcgttataac atatttacgt aaatcaatct tatgtctatt
    37441 attattaaat cctctgtata tcgtttttct tctattcatt agtttgtccc tcccaaaatt
    37501 atattattaa taaagtatat ttttaagagt cacaatttat gaatataaat aattatttct
    37561 tctaattttt taataagaaa ttgtacttac taaacaaatc ttcttgcacc aagtaatact
    37621 ttagaccaat atgaactagt taaattatcc actttaacct tattaggatt agtctgagta
    37681 ccactacaat ggataaattt actatttcct acgtatatac ctacatgatt agttgttcca
    37741 tcaccatcag tgtcaaaata aactaaatct cctggtgcat aatttcccat agaaattgca
    37801 cttccaaaat tagcctgttc tctagaaact ctaggaatag aaacgcctac agaatgttta
    37861 aaaacatact gtgtaaatcc agaacagtca aatttatcag gtcctgaagc tccccattga
    37921 tatggtattc caataagagt atatgcaaaa tctacgattg ttgaaccttt tgctgtagat
    37981 ttatctgaat ttccagtaaa tgatggcttt gagttactat tagattgaga attttggtta
    38041 gatgatgaat ttggtttttc ttttgtatcc tcattagtct cagaaatata actggcactc
    38101 acccatccaa ttgttccact cgataacttt accttatacc aaccattact tttagctttt
    38161 aattcaacaa catctccacc atttaatttt cctattatag aatagcttgt cccaggacca
    38221 cttcttacat ttaaactcac agcagatgtt accttaccat tcttgccttc aattgattct
    38281 tcgctagatg gttttttatc agaattatta gtactatttg aattactaga attgttcgtt
    38341 ccaacatctt ctgatgtttt agaaatatat tggctactag cccatccagt aactccattt
    38401 gatagtttta ttttatacca tccattgctt tgttctagta actttactac atctccatta
    38461 tttgctttac caactaatga atagttagta cctgcaccac ttcttacatt cagtctagaa
    38521 ctcacattta ctttaccatt tccactaata gttgtcccac ttgaagttgg attattttga
    38581 cttgatgtac cttcagacga accacttgtt gatatgtatt ttgcacttcc ccatccaaca
    38641 acagaactcg atacccttac tttataccat ccattagact tttctaaaat ctctacagtt
    38701 tttcccttgt ataattttgc taccttatcg caatcagaac ttggtccact tcttatattc
    38761 aaggcacttg ctgttactgt tcccttttca agagcacttg catttatgga gctaacagat
    38821 actgctactg ccccaattcc taaagctgct atagcttttt tcactactat cactcccccc
    38881 aacaaatttt tatagtcata aattcataac attattatcc atttttagtc cctacaattt
    38941 aatgataact tattttctta ttaattgtaa agaaattata aacatatcct taaaattagt
    39001 gatttttagg aagtatttca atccaataat tatctgggtc cgctataaaa taaattccca
    39061 tactttcatt ttcaaaacat atgcaatcta aatctttatg aagttttttt gcctcatcaa
    39121 actcctcaac ttctaaagcc agatgaaatt cattgtctcc taaattatat ggtctatccc
    39181 aatctctaag ccaagttaat tctaatgtat gactagtaat gccatctcct aaataaacta
    39241 atataaaact tccatcttct gcttccttgc gctttacctc tttaagccct aaagcttttt
    39301 tataaaaatc taaacttttt tctaaattag taacattgaa attgttatga caaaaattaa
    39361 acttcatttt gaaatcactc tccaattttt atttttttta tattatatca ttattataaa
    39421 tttatttaaa tcatattaag ttaaaaattt gaatgatgat gaccaattac caaatcatgg
    39481 taaattattt aaaagaatat gtcaaaaaaa gaataagctc tttcaaatgc ataaaaaatt
    39541 tatacccaag ttatacttaa tcatgcaaat tggcagaact tattctttaa aactctaact
    39601 tttaagtttt tctttaataa tataatttat tacttttttt ctaatatctt cagcattata
    39661 atcatcttct acttctatat ccaaaagttt tgctattgac ctcatatctt ctctagtctt
    39721 tgacctatag cttgatatta attcatcttt tattttctct ggttctaaaa gatattcttc
    39781 atctcctctt ttgaaaacaa cgtatttttg agcatatgaa cttctattgt agtatatata
    39841 tcttgatatt tttcttaaaa tttgattata tgaaagtcgc ttgttaatat caagttttaa
    39901 gtactctgaa aaagcaaata gctctttttt tgatttaaat aatgcttctt tctttatagt
    39961 tcttgatatt gaagttacac tttcttgata tgcacacttt tttaacattt ttaaatatac
    40021 tctattaaga ccaatacttt gattgaattt ttttatatta ttattaataa tcttatccaa
    40081 gttaccttca aagtattttt ttgctccttt ttgtgataca taactctcaa gttctttgtg
    40141 ggccatatca agtatcattt tttttaaatc ctctttatat cccctagatt ccatgatact
    40201 ccctccctta gctatttata gtatattcaa aaagctcaag aaatgtgagt cattacaaaa
    40261 tttttttact ttttatgtat atattaattt aaaaggggag ataatagtaa tgtaaaatca
    40321 aacatactca atctccccca tttttagacc tctattactc ccccaataga ggtctttttt
    40381 tagtttatca atttctattc aattcccttt agttcaaatc ctaattctgc tatcacttct
    40441 tttaattttt caatagatac atcatctttc atatctacag atgcacactt attttctaaa
    40501 tctacatcta aaacttcaat accatctata tcttctgtta atgcagtttt aagatggttt
    40561 acacaatgac cacaactcat accttctact aatagtttct tcttcattta attacctcct
    40621 tataccctat aagggtattc tatcatatag tttatacttt ttaaatagaa attaatcatt
    40681 taatttgatt cattcgattt tatatctaaa ttagatattc cctcatatac atcaaaatca
    40741 tttggagtaa ttttaccttt taatctacta ttacttacaa tatagttgtt tctcacagct
    40801 aaacttataa atgttgcatt atcttttata tatgtctgta catctttgta tgcttttttt
    40861 gtttcaattt cagacgtaga acttgtaagt gcagacatat atccatttaa cttatcatca
    40921 gtatatccac tactcgctat aatactactt gcatctggta taattgatag tttccatcca
    40981 actaatgcta aatcataatt tttagaatta agagcatttt ccatatcctt atctgagagc
    41041 tgatttactg tactatttat tcctatagct tttaagtttt catttattat gtttgcagtt
    41101 tttattctct cactattatc tctgtttact attatcttta agttcatttc agaaagactt
    41161 ttctttactt cctctttttc tctatttttt ttctcttcag cttctttttt gattctatct
    41221 tcttctgctt ttttaacttt tgcttcttct tctggcgtaa gcttctttga gttcttttta
    41281 gctgattttt tctgattttt tactttattg tcattttgat ttactgagtt tacatttgca
    41341 tattctacct tagcaagaca ctctatcgcc ttatctttat taaactctaa atccttcatt
    41401 tcactattat agtattttga ttttgaatta agtggaaaat taactggagt agcatcatcc
    41461 atataacctt cactaataat tctatcttta tctatagagt gtgcaattgc ttttctaaaa
    41521 ttaacatctt tgaaaaaagg attattataa ttaaataata cacactcata atctcttcct
    41581 tgatattttg ttatattaaa ctctttttct tgaaacttac ttaaatcatt taatgtaaca
    41641 ttggttatat cactatcaag agccataacc atagatgtcc ttgcatcttc attaggcacc
    41701 atacctactt ttatgttctt catggttttt ggaacttctt catagtaatc tttgtttaca
    41761 gataaaacca ttccttctct ttctgtatat gattctattt tatatttccc atttcctatc
    41821 atattattat tcttgtcatt tacatctttt gaatctaaat gattttgaga tacaattgga
    41881 aaaattaaac tatcaattga aaaagcatat ctagctttaa attttattgt aaaatcttta
    41941 tctgatttaa tattcacaga agctatattt ttagtaaatt cattatatgg actatctgca
    42001 cttttttgaa ttaaaccaat agtatagttt acatcatttg aagtaacatt ttttccatca
    42061 tgccactttg catcttttag ctttatatct atactcattc catcctgagc tatattatac
    42121 tccttaacta attgaggaat tacattgtag ttctcatcta tagtaaataa actatcataa
    42181 actaaattca ttatataacc aacagactta tcagtattag ttataggatt tatagttttt
    42241 ggagttacca ttgttaagtt tatatactta gcatctatag aactttgact ttgtaaactt
    42301 gaatcactac tactttgttt tgttttagta ttactacaac cagctaccat aagagttata
    42361 gctaaaacga ctgttaagac ttttattctc ttcattagtt ttttcctttc ttgccatata
    42421 tcttattata atgttcgtaa ttatttttta ctttttctac atattttgaa gtttgcttaa
    42481 aaggtatatc atgtagattt tcgccatctt tactatattc attattttct aaccattttt
    42541 tcacattacc tgaaccacca ttatatgcag ctatcactaa atctaattta ccaaattctt
    42601 tgtataactt acttaaatac caacagccaa gtcttatatt agtctctggg tcgaaaatat
    42661 ccacattttt taaatttagt tcctctgctc cccaatctct agttatgtct aatatttgca
    42721 ttaatccttt tgcttccttt tttgaaacag cagaactatt aaacttactt tcggctttaa
    42781 taacactgta aactatattt tcatctaaat taaattcttt cgaatacttt tctacatact
    42841 ctgaatattt tttaggatat aaaaatttat gtattacttt gctttccatt aatagtgccc
    42901 caaataagat tataaaaata gatagaatca atactttctt actattcacg ccaattctcc
    42961 ttcatgtact caataaattt atgtgcttta ctttctaatt cagttattgt tcccgaatta
    43021 tctattatat aatcaccata tttggatttt tcttcctgac tcatctgtga atttatccta
    43081 ctaagtgctt cctgctcact gcaattatct ctcttttgta ttctactaat ttgtacttct
    43141 tgtttacaag taactactaa tagcttgtca accatatcta aaaaaccact ttctaccaat
    43201 attgccgcat ctaacactac tatattttcg cctttttttc tcaatttttc tatttcactt
    43261 attatttttt ctcttattcg tggatgagtc aagttattta attccttaag tttttcttca
    43321 tcactaaaaa ctattttccc aagagctttt ctatttagag ttccatcatc attttttata
    43381 ctttgcccaa aatggacaaa tactttttcc aataacaact tatcctcaaa tatcttcctg
    43441 gatataatat cagcatccac tataggaatg ttaagatttc taaatatatt tgataggctg
    43501 ctttttccac agcctattcc tcctgtaagt cctaatatca acataaaatc acctactttg
    43561 tctcatacca agaactacct acatttaaat ctacatctaa gtgaacatcc atatttacag
    43621 cattttccat ttcttctttt actattttct taactatatc tatttcatca tcaattgctt
    43681 caactatcaa ctcgtcgtga acttgtagta taagttttga tttcaaattg ttctcttcca
    43741 attttttatg aacatttatc atagcaattt ttattatatc tgctgcactt ccttgtattg
    43801 gtgcattcat tgcaaatctc tttcctctat ttctttccat aaaattgctt gactttattt
    43861 ctggtatata tcttcttctg tttaaaattg ttacagcata tccatcctca ctagcctttt
    43921 ctactgttgt gtccataaac tcttttattt tatggtattt agcaaaataa ctttcgatat
    43981 attctttcgc ctttggaaca ggtatattta aatcatcagc tagaccaaag tcacttttac
    44041 catatataat accaaagttt acagctttag ctgcacctct caattcacta gtaacatctt
    44101 ccataggaac attaaaaact tgtgaagctg ttttggtatg aatatcttca tgatgtttaa
    44161 atgcttctat catattttca tcctgactca tatgtgcaag aactcttagc tctacttgtg
    44221 agtaatcggc atcaaccaac ttacagttat catctgcaat aaacactttt cttatttttc
    44281 tacccatttc catttttata ggtatatttt gaagatttgg ttctgtagat gaaattcttc
    44341 ctgtagtcgt tatagtttga ttaaatgatg aatgtattct attactttct ggatttatta
    44401 tatttaaaag tccttcaacg taagtagaat ttaactttac tatttgtcta tactcagtta
    44461 tcttatctat tattggatgc ttatctctta atttttctaa aacatctgca ttagttgaat
    44521 atccagtttt agttttttta attattggaa gctctaattt ttcaaataat ataacaccca
    44581 attgttttgg tgaatttata ttgaatggct ctcctgataa ttcataaatt tccttttcta
    44641 atgtagctat tatttcttta aattcaacac tcaattcatc caatatactt ttgtctactt
    44701 ttataccttc atactccata tgacctaaga cttctactaa aggcatctct acatgataaa
    44761 aaagaccatc catactcata tccattagac ttctttccat cataggaact gtttctttaa
    44821 ctgtgcatat tatttgaccc atatactctg ctaaatcctc aaattctaaa tcctcatagt
    44881 ttttagattt aacacccttt cctaataatt cttcttttga tttaaccttt tttgataaat
    44941 acttcatagc tattgcacta caatcatatg aggtagatga agttgaatct attaagtact
    45001 cagctattgt tatatcaaaa tacatatttt ctaagtttat attatatggt tttaaagata
    45061 tataatcatc ttttaaatta tatccatact ttttaatttc attatttgaa aatatactat
    45121 ctaactttga cacttcattt gaatctacat aatatatttt ttctccatct acacttataa
    45181 aaatatatat tatatttttt tcaagtatat taccttgtcc tctaacagtt tttaaaataa
    45241 cttgtccttt tttgtttact tcatctataa atttttctat atcttttaat tttaatattt
    45301 ctattttttc ttctacgtgt tctgtgtcat ctgaatcgac caaatcaact aatcttgata
    45361 agagacttgt aaatccaaag tatctaaatt tatctaaaag ttcatcttta tcataatcgc
    45421 caaaaatcat actatctaaa tccacttcta ttggcacatc tcttataatt gttgcaagtc
    45481 ttttactttg aatagctatt tctttgtttt cctctatttt cttttttaca ctacctttaa
    45541 gcttatctgt attctctatt agattctcta tactagaaaa ctcttttata agctttatac
    45601 ctgttttttc acctattcca ggtactccag gtatattatc agacttgtct cccataagac
    45661 cctttaaatc gataaattgt gttggagtca tctcgtattt ttcaattaca gaattaaagt
    45721 tgtattcttc aacttctcca actccttttt ttgttataag agtagttgta ttgtctgaag
    45781 caagttgaat agcatctttg tctccagtta ctatgtatac cttatatcca ttttcttctg
    45841 cttttttaga aacagtacct ataatatcat ctgcctcata gccatctatt tccaatctat
    45901 ttattctaaa tttatctaaa aggtctttta atggctgtaa ttgttctgcc agttcatctg
    45961 gcattccctt tcttccagcc ttatactctt tatattctaa atgtctaaat gtaggtgctt
    46021 ttctgtcaaa tgctacactt atatgtgttg gtttatatat atctatcatt ttaaatagca
    46081 ttgttgtaaa tccatatatt gcattggtct ttagcccttc tttattattc atttctggaa
    46141 gagcataaaa agctctattt attattgaat ttccatctat tataattaat tttttttcca
    46201 aattatatca ctcctaaaaa ttatctattt ttgtaacata tatattatat accaatatac
    46261 tgttatttaa tattcccata ataataatag tatattaaaa ttaaatatgt tgcaaacaat
    46321 atattttttt gctataataa tatacatgac atgcccgagt ggtggaattg gtagacgcag
    46381 tggactcaaa atccaccgga ctaatactcc gtgccggttc gagtccggcc tcgggcacca
    46441 ttaaaaactc ctaaagctat ttattcaata gattaggagt ttttttattt ttgatttaaa
    46501 ttttaagtta ttttttgaaa ttactgtgtc tttttaaaat ttatataatt atataaattt
    46561 atcttctagc attaatttaa aaactaattt atttttatat aattttctat tttatatcat
    46621 aacttattac aactttttat ctaatttatt gtatacaaca acactacaaa taaattgaaa
    46681 taaaataagc aatccaagaa caactaaaac ccacttgctt attaccatta cagaaaatac
    46741 actcaatata gaaacaataa atataagaat tacaagaaca taggacatga tttgattagt
    46801 gatacgtcca gctttatcac gtaacataat tcgtctttca tcattcatct ctatcctttg
    46861 attttttatc ttttcttcat aatctactgc tctttcaggt ttactccaat acatatattg
    46921 aagaatcata catacacctg gacctaaagc agctccagat aatccccaaa taaaagcctc
    46981 aaatgaaaac tcagtaaatg tagccaataa aacaagtaaa attccacaaa taaaatatat
    47041 aattccaaca tataacatat tctttttcat ttttactcct cctcataaat aaatatttct
    47101 tcaatactca tatcaaagta tctagctatc ttaaaggcta agatgataga aggattatac
    47161 ctaccatttt ctaatgaacc tatagtttgt cttgatactt gcaaaataga agcaagttct
    47221 tcttgtttta tgcctctttc ttttcttatt tcttctaatc tatttttcac atctatcccc
    47281 ctttaatatc tcaactacgg aaagtctact ttccataatt atacaactat attccattta
    47341 tgtaaagctt actttccata tttacaaaaa aataacagac tattttcagc ctgtaatttt
    47401 agaatttcat gttgtttatt aattttaatg ttatttatta attccatatt atttattaat
    47461 tttaacttgg tttctgaatt ggaatccaaa cttctgtagt ataatcagga gcattgatat
    47521 cgccattggg ataaagttca atatctggta aattagcata cacataccca gaacttggta
    47581 accactcagt aataattctt ttttgcaatt cctgtattgc cagagcattt ggcattggag
    47641 aaacacattc aaatactgcc catgtgcctg atggtattaa gtattcatga gtatcttcta
    47701 atacaggctt atctgttgga gcagcaatat aataatctaa acccttacta tttgtacaca
    47761 cactaactcc aagcaatcca caaggttcat tatttattaa ctctgaaagt ttatcaattg
    47821 ttccattttt tatgcattta gcccacaact taggaacttt ctcaaagcac tcttctacaa
    47881 tcatgttcat actttctttt ataccaacaa tacgaaaccc tttcttttct acaatacgat
    47941 attgcatttc tatatttcct tttacataaa tgcttaatgt catcttagga tatgctttta
    48001 aaacaacacc ctttgctcta gctgcagatg gagaaatgtt atgtatattt tgaaaagctc
    48061 tattaaaaga tgttggtgaa tcgtaaccat acttctcaga tagttctaaa attttgatgt
    48121 tactgttctg aagttcaaat gcagccttag tcatacgtct tcgtctaata tattcagata
    48181 atggtacttc tgcaatatat gaaaacattc tttgaaaatg aaaggttgaa caacatgcaa
    48241 tttttgctgc ttctgcataa tccactccac tttcgagatt ttcttcaata taagtaatag
    48301 cttgatttaa tttatttatc cattccattt gatattacct ccaagcaaat tatacagttt
    48361 aataaaataa tatacctctc tattcatgca caaaattgta agcatttaaa tttataagca
    48421 tttttcaatc aatacaataa atataaaaat tgtattatac aataaaaaag ttcaataaca
    48481 ttatattata aaatgttatt gaacttttta taaaaattaa tttaagtgta gttaaattaa
    48541 gtattacact atcttatata ttacaatatc ctactatctt atagtcttct ggcactttat
    48601 aatctttgtc tggagtacct aaattccatt ttaaatcaaa catagttgca cttattattg
    48661 ttgcaaaata aagcataact ataccaatgt ctattttttc ttcatataaa ttagtatgac
    48721 catcttttct aacagctaaa actacagtgc ctccatcaac tataaatctc catggttgtc
    48781 tatttaatgt agatggagct aatcttgcat agtgaaatgc atcaagaagt gctctttctt
    48841 ctaagatttc tatacttgca acatttcccc attcgttcat ataaacaaca tcttctacac
    48901 ctagtctagt agacccctta tctgttacaa cttgcatatc agattttgaa taattttgcc
    48961 cagtttttgt agcatttaat actttagcat tacttacatt ttctccatat ccaatagcta
    49021 taattcctgt tacctcttta tctgaattaa ttccaagttt ttcttttacc ttttttccat
    49081 cttcaaaagt aacccaacaa gaaccaattc caagttctgt tactttaaag attatacttt
    49141 ctcctatgta ccctgaattt tcaatgtaat tatcttttat gtcagataat attataatat
    49201 aacttggaga atctatcata tttcctttgt accctgctac tttatctaaa ttattaaaaa
    49261 cttcacttct attcataatt cttacatcaa catctatttc tggaactagt ttcttacaag
    49321 attctgagta tttcttaatt tcactaagat attcttcttt aatatctgtg ttcttatatt
    49381 ctctaacaga ctttctattt aatatgattg ttttataatc cataatttta tcctccccaa
    49441 actagtattg tcttaattat atacccatta atatattatt ttactcattt atttaataaa
    49501 taaagattac aattttgtaa ttattattta ttaataatat tccactttta cattaataat
    49561 gaccagttgt gcaaaatcca ttgccaacca ccaaataaaa acatattata aaggtaatat
    49621 aaaataacaa acaaatctta acaaaaaatt aaactttttt tacacgaaag aaggcttccc
    49681 gttgccttct ttatttttat aaaaaaataa gctgtttcta aaatgatttc acatttttga
    49741 aacagccaag ttattctata atgacaacta taatttgagg atgtgaggga gagttgtcat
    49801 tataaattta gggtaattat attatgtttt aagctagttc tttctatatt tatataatac
    49861 attttatata tgaattttat atgagattgg tataaatgat atatgaaatt acatatgttt
    49921 atattatatt aaattttatt attacatttt ccacaacaat actcatatgt ctccacatta
    49981 tttggaatat ttacaaatac aaattcttta cagttctcac aatactttaa tatagatata
    50041 gttccacaag tgtaacattc aaatattaca caatcctcat tttcctctac tatttcaaac
    50101 tttttactat gttcaccaca ctctgggcac ttgtgattat ttacattcat aaatactcct
    50161 ccttattagt atagttacat gtcaaaaact taattaatgt attctatatt acttattata
    50221 tattctataa taaatcatat tctaatataa taaaacaaat taaataaaaa aaaaccataa
    50281 attttaaatt tatggattaa ttcttgtaat gataactcta tttgaggatg tgagggaggg
    50341 ttatcattac aatagggtat tatattatgg tttttaagct agtctttcta cacctcatat
    50401 gatacatatg ctatatgaat tttatatgat ttttacataa atattttatg aaatttaaat
    50461 tttttagtca ttttgaaatt tgtaaaaagt tctctaaaaa tccacagcat cataaacagc
    50521 caaaatttaa gtagaacatc ataaaaaaat ttgtccaata attttaacaa gtaatctcac
    50581 ctcattataa ttattgacac aaacttaaat aaacaatcta taaatcttct ataaatctta
    50641 aactttcccc ctattgatac acaatttcta gtttgtcaat tttattttgc atttctaaga
    50701 aaatttcttc aacagaattg ttaaatatat tgcctaaata gattctttta tctttttgaa
    50761 atgaataaga ttttacacta ccatcagaag aaatatataa atgactccac tctgctccat
    50821 attttgaaaa cctctccttt gtatctttaa acctcatatc ttctattttt attgcttttt
    50881 ctctgtctcc tcttactaca gtgtgacccc tttttattaa actggttatt tttatatctt
    50941 cttttaattt aatacgtgta tcatcatttt ttgaatatac tttccatatc actttatgac
    51001 gataattatc taattccttt attaagcttc tatcaatagt aaaactatct ttagttacca
    51061 caattaaatc aaaatggtct aacatatatt gaaatatttc tctcatactt ttatttttta
    51121 aaatatcctc accaataacc tctataaatc tacagccttt ctcatacatg cagttcaata
    51181 aacttgtgat ttcagaaaaa taatcatttg tattattatc tccatctatt tctatagtaa
    51241 agtacaaagg agtaatgtat gatttatttc ctgttatctc aaatacactt gatagtgtct
    51301 gtatatcatc ttcaaacgtt aagtatacat tctcataaaa cataaaaaat tccataactt
    51361 tttctttcat attatggata tttctatatc tattggataa tatatttatt atatcgtcag
    51421 tagtattttt cccatcacat tcagccagta taacaatttg cttccaactt aagctatcca
    51481 catttccgat acttttttta tatccatctt cttcatcata aataggacca attataatgt
    51541 tttcttttgg ccacagatga accctagttc catcttttaa tttcggatat ttatttttta
    51601 cagactcata aaatctactt gtatcaccca tgattcttta ctacccccca tagtttaaat
    51661 attctatgct aaaaatagca ctttattttt attaattcaa caattattca aaattggtca
    51721 ttatatatgt ttaaatggat ttaacatcaa ccttatttat atgttattga tgtttatata
    51781 tgttatataa aatcttattt ctcatacttc aatgtaatct taactatctc tggtttacaa
    51841 ttaagcctaa ttaaaacttt tgagcctcct aagcctttat ttaaaatcat agtgctatta
    51901 tctttttcat attttcccca gtcatactta ggaaagaact ctctatttgg agataataca
    51961 cctccaacaa aaggtattct tattatacct ccatgaacat gtcctgctaa aaccaaatct
    52021 gctccccatt ttgcatattt ttcaaagaaa aatggtgtat gtgctaaaag aatgttatat
    52081 tcattttcat taactttacc tagattgtct tctacaaaat tgtcacttag ttctaatttt
    52141 ttgctattag caaataaata tggatagcat tccaatggta taattaaacc atataaatta
    52201 agatggctat tacctctata tattcttaca gattcatttt ccatatttat tattttcttt
    52261 gaatatagtt ccttaaaata atccttatat agatgtttat acttttttat taaagatttt
    52321 tgttcatgat ttccaattat atgatatact tcatattttt caaccaaatt atctattaaa
    52381 tctaatgcaa cttgaaaatt tttattttct ccatctacta aatctcctgt tataaatata
    52441 acatctgggt ttaatgattc aattttatct atcaatacct tattattttt atcaaacacc
    52501 ttattatgta aatcactcac ttgaactatt ttaaagttat caaattcctt tggaattttt
    52561 ttgttgaaaa tagtatattc tgtcgtttct atatgctgag tttcatactt atacagaata
    52621 tacaataata ttaatactaa tactgtcatt gaaaccttta taatcatatt ctcttcctct
    52681 ttattctatt ttgttaatat tattataatg cttttatgtt ttttttaaaa caataacatc
    52741 atcacataca tataaatctt ttcgacatta taaaaataag ttatcctttt ctaaaatttt
    52801 catttatttt agaaaaggat aactataaaa ttatctataa acctctatat tgtgaaatat
    52861 agtttttcct ttatttaaat ttttatatat atgctctaaa gttccatcaa acctaataga
    52921 atgtccagac tctaaaatat aagttttccc atccacatta aggtcaagtt taccttctat
    52981 tacgagtaca tactcttcta aaacatgact gtgtgtagat gaaacatgag tacagttttc
    53041 ctcaagctca attataaaaa gttcaaacct tctttgaggg ctaaaagggt atattggata
    53101 taacttcatg ttcccttttt gctcaacaat aggctctact tcttctttct ttataacctc
    53161 ataggtattt gtattttcat ttaatagtgt agtaaaattt attttaaatc cagatgaaat
    53221 tttccataaa gtagatactg tcggacttga ttcacctctc tcaatctgtc caagcattgc
    53281 tttactgaca cctgtcatct ttgaagcttt gtctaaactt atgcccattt cttgtctcaa
    53341 taaccttaaa ttttctccca cattaggaaa tttattatcc atgttaaaat cctcctgata
    53401 gctatttgta ttctatgatt tttactcctg taagttctac tatctctttt aattttaaca
    53461 tatgattgtt acaaatttca tcaaattcat atatataatc ttcaagccct aatgccttat
    53521 atttatcgat tccaaattta tgatatggaa gtaactccat cttgcctcct ggaacatttt
    53581 gtttgacaaa taatgcagta tttcttatat tttcttcact atcatttact ccataaataa
    53641 ttggcactct tataaccata gatttattca actttgaaag cctacatata ttatctaata
    53701 taattttatt acttactcct gtatattctt tatgaatatt atcatccata gattttatat
    53761 ctacaaatat gtggtctatt ttctcaaata catcatttac cttattccaa tcaaaatatc
    53821 cacaagtttc aattgcaata tttatgcctt tatcataaaa aatatcaact aattctctta
    53881 aaaaatcaat ctgaagtgta ggttcacctc cagaaaaagt tatccctcca ttagattcaa
    53941 agaaaaatat aaaatctttt tcaacttcct ctactatttc ttcaacactc atttcttcgg
    54001 tcattataca aatggcatct ttaagacata ccttgataca ctctccacat aaatcacatt
    54061 tattatttat ttgcgctcta tcaaatagtg aacttatatt ttgaggacaa acatctatac
    54121 atctattaca ggatacacat ttatctttca taacacctaa ttttactatg ttactccagg
    54181 aatctggatt agaacaccac ttacatttta atttacatcc ttctaaaaat atagtagtcc
    54241 ttattccatc tccatcattt actgaaaaat gttgtacttt tacaactcta ccttttttac
    54301 tcataatcta cctcataatt aaaaattgct atgtgaattt ctagctatta tagcatcttg
    54361 catttctctt gataaattaa caaattgagt actatatcca gcaactctca ctaataaatc
    54421 tttatattct tctggatttt cttgagcttt taatagagta tcatttgata tcgtattaaa
    54481 ttgtacatgg aatcctccta aagcaaaata tgatttaatc atagatgtta gatttctaag
    54541 tcctctttca gtttccacca aatcttgatt cagtcttaaa tttagaagtg ttccaccagt
    54601 atgaacatca tgatttattt ttgatgcaga tttcattgct gctagtggac ttgttgtatc
    54661 agtaccaaca aatggagaaa ctccctctgt caaaggtttt gtcgcttttc ttccacaagg
    54721 agttgcgcct acgatttttc cagttggtac atagtttgat atacccataa atgcactatt
    54781 aaatttagag ccaaaaatat ccttgtattt tctagtttca gtataataaa aatcagaaac
    54841 ttcaattgct aatgaatcta cataatcatt atcatttcca tacttaggaa catccaacgc
    54901 aagttttctt aattcttcat aaccttccca atcattattt agtgcttttg ttaattcttc
    54961 taacgtagtc accttatcct caaatactaa tttttttata gctgccaatg aatttgaaac
    55021 tacacctaac cctataccag ttaatacagg acctatatta tattttgctc ctcctttact
    55081 taaatcaact cccttatcca aacatccatc tacacaagta gataaaaatg gtcttggtac
    55141 catttcttta tgaatttgtt gagcaacagt agttcctata acagaacttt ttacaagata
    55201 tgctaattgc tttaaaaact catccttaag ttcttcaaaa gaagtaaatt ctgtgatatt
    55261 ttttgtatct aaccccattt tttctccagt taatctactc ttaccttcat tcatagcata
    55321 ttctaaagct gctgcaaaat tgatatttac ggctgaagtc cactgtcctg tttttctaaa
    55381 atgaggaact acacaaccac agttactcca atctctagca tcttctgcat catatccatc
    55441 ttgtaataac atttgtgccc ctgcactatc gctgtgtata gcagggaaac cagttccttg
    55501 ttttaccaat ttagcaactg ccattacgaa attgtctggt gctccttgac tcactctaac
    55561 acttaatcct ggttggtgag tttttacact ttctgtagcc tttaaacaca tatatgagat
    55621 atcattcgtt ccatcagtac catttctctt tttaccacca acagtaagat tttggaattg
    55681 attatatcct gcaaagtaat ttgctgtatt tgctgatata gtccataccc attctgataa
    55741 cttaatccaa agtgcttcta tcaattcttg agcttctgat tcagttattt taccttctct
    55801 agcatcattt tcataatatg gatacatata ttgatcaaat ctacctaaat ttaatgccaa
    55861 tggattttct gatagaatac cacctaattg aacaaaccat acaaattgta ttgcttcata
    55921 aaaattagtt ggagggtttg ctggtacatt catacatatc tctgaaattt ttataagttc
    55981 taactttctc ttttcatctg tttcttgctt actcatctct tttgcaagat ttgaatatct
    56041 ttgtgccaat gtcataatac cctgtgcaac tatagaaaca gatttgtaaa aatctatctt
    56101 ttcaatattt tctgaaactg atatatctaa ttctttaatt tttttatctg catcttcctt
    56161 aatcccttta tatcctttta caaacaatat atcttgataa tctggtgtta cttcaccaac
    56221 tgcctgtctc cacttagaat cattatctat tattcctgta tctactaata tcttagctgt
    56281 atcttctggg attcttgcta agaaaacttc ttccaaagat tttcccttcc aatatggaaa
    56341 tatttctttt cttataaatt ctatttgttc ttttgatatt acatatgggt cttgagttct
    56401 tttatcaaaa gtatccattt ctttgtctac ccattgccaa gaaaactctg gtgtcaatat
    56461 ccctgttctt ctaaattttc ctgctgtacc tactattaat tcattttcaa agatttttat
    56521 aggaagttct ttacatgtct ccaaaaaagc tttagctctt cttatacaga tagcttctcc
    56581 ctcagttttt ttatgtgatt ctgtaaaaat tctagccctt tcacaacata tcataggttt
    56641 agaattaata tattcttgtc taaatgcttc tattctatta gtttgactca tttaaaatcc
    56701 ccctttggat aatatatact acatttttgt atgttataac atactttaat tatattatac
    56761 tgtcctttag cataaaatgg aagcaaaata acttattttt tttcatctac cttgaacacc
    56821 tatatattaa tacttttttt gttaaattta taaaatctaa aaaaattttc tatgatatat
    56881 tttctatatt gttatagtta tgtctaatat actaatgatt tgaaatatca aatttgtata
    56941 ctagacttag ttttaaaacc ttagttgatt gtttaacttt gttaacttca acatgtatat
    57001 ccttcatata aggaagctct acaacaccta ctcccttatt aaagtcttct tcaaccatag
    57061 aaaaaatttc tttctcagtt aagttcgtat cagtcaatga tttaaataaa ttgtatataa
    57121 atttaacata tttatcactc tcagataact tatcatcttc tggatactct ttagcacaga
    57181 gaatatctat tgttactttt ccttctttat cgtcttcaga catagaatag aataaacttc
    57241 gaatattttt ttcaggtggt ttttcagcac catttataga tactacaaag ctaccatctg
    57301 attttgagtt agtaatacta taaccactgt ctattatagc tttcgaaaat ctttgagata
    57361 aatctttttt tacttcttct ctacctttcg catcattaat cttttgtgag taattattaa
    57421 atagctcaat agattcatct tttttatctt caacttgttc tttcaatgaa tctttactaa
    57481 tttgttctga gctacttaag ttcttatcag atacaggaga catacaccct accatcaatg
    57541 ctaagctaag atttaaagcc acaattattg ccactctatt tatattcctt tttactttca
    57601 tacacaatcc tcccatcaca ctaaccgctc taaagtatta ttcattgcac tagaatatat
    57661 ttatattttc tttatattaa agatttatta ttcaatacat atcaccttcc tatccaattc
    57721 ttataactta ctattagtaa aattataagc tccttaatac agaaataagg atgttatctt
    57781 ttgttattaa aagataacat ccttattttt ttgtatcaat acatatgtcc caactggtaa
    57841 cttaatcgcc ccaactgtca gtatcttggt ccaatggtat atatatattt agtataaatt
    57901 tatcgatact attttcaaat aataattctc cattatattt ttgtaaagaa gatttaatac
    57961 tctgaatacc tattccatgt acgaacttat ccattttgtt tgttaacaat atatttttat
    58021 taaaagtaag ctgatttagt ttactatttt cacatttaag tacaaaaaat gaccttgtta
    58081 tagttccttt aattctaata tatttattaa tattttcatc cactatttta tcacatgctt
    58141 ctattgcatt atccaaaata ttagaaaata tgcttgacac atctataggt tttacaaaat
    58201 ttacttttga aaaatttata gagcaagtaa atttaattcc tttttttaaa catacttcac
    58261 tcttttcgtt gattataatg tctaatatca tatttcctgt attatagata tatttaaatg
    58321 tttttatttc atcttttaaa ttgttaacat attcattaat ttctttgcta ttgtttctaa
    58381 ggttgtctat acaataaatg tgattattta tatcgtgata tagtttctta actcgcatat
    58441 gagattcttg aatacttaaa taatgcgcat attgcatatc taacttattt tttattaatt
    58501 cagcttgaga ttttaatcta ttctctttta ttatttcttt aaacaatagt ataagaaata
    58561 tactactagc tcctaccaat tttggattaa caaaattatc aaaatagtat ccttctgaat
    58621 gtaatgaccc aaaccgttct aattttttta atacataatt tacaagcata ttcattgtat
    58681 taactaataa ggcaaataat aaatatacat aatttctctt atcttggcta attaatttta
    58741 tagactttct aaaaaagcta aatataagat atataagaat aattattaaa ttactatata
    58801 tctgaatttt tatagattct aagtaaggta ctaataaaga ttctttagat gaaataacat
    58861 tacctgtaat tgcaaaaaat agtattctat atataacagc atccaaacat ttataatata
    58921 cactaaatag ataaaagaaa atggaatact taatataacc catcttataa aaataattat
    58981 agtatcctcc ccaagcaata aatgctaata tataaaataa atagtttata ttactaaagt
    59041 aatttcctcc attaaataat ctacttatct gaattataat ataaataaga aacaagacaa
    59101 tatagaatac tattttttca ttttttttac tatccttttt ataaattaaa attccgttca
    59161 ttatagaaaa ctgtataaat atagataaaa ctactaatag ctcagtcata aagtttagca
    59221 tattgtatca ccaagaaatt ttaagaattt ctccttaact tctttatatc tatatctact
    59281 aataggtacc tcttctccac tttccagtat tgcagtatta ggctttatat tttcaacata
    59341 tcttaaattt actataaaac ttttatggca tcttataaat ttatatggat ttaattcttt
    59401 ttctattttg cctaaactat attttatatc aaaattcttg tttattgtgt gtattagcat
    59461 atcttttttt tgaacttcta tatattttat ttcatttaaa taaatcttat atgtattaga
    59521 cttatttttt attgtaatat gactttcctt atttatttca atatctttga tacaagttag
    59581 cacatgtttt tttagttctt ccaactctat tggctttagt aaatacctat aagctcttac
    59641 ctcataacct tcttgcacat aatctattaa tgaagttaca aatattattt ccatttcatt
    59701 gtctacttct ctaatctttc ttgcagtatc cattccattt aatttttcca tttttatatc
    59761 aagaagtaat atatctatat cttttaaatt actttccagt aactcttctc cagatatata
    59821 cttttgtatc tcatattcta tacttatatc ttttaatata ttctcaaggt aatcttctat
    59881 taaaagttgt gtttcttttt catcttcaca aactgctatc ttaatcataa aatgcctcct
    59941 tacataattt ctagttaata caattaaaat atttataata tatttaaaga taattttgta
    60001 tatctgatac tttaggataa catttttaaa ttatttttat caatactcaa tcccatacaa
    60061 ctattatttg atatttcaat ttaaagacag aaattactat aattaaaaac agtacttact
    60121 gacaaaagaa gatgtcttaa aatattaaaa aaataatttt aggacacctt cttttattta
    60181 tttaaacttt ttaagcctta aataatacat taatctccta tataattgta ctgcatataa
    60241 aatcaacaat ataatctgta gtacaatagc agttacagat gctccataac cactatataa
    60301 atttcttatt tcaatattca ataaatatat aaatattact actaacataa agaatataaa
    60361 catattcata aataaaattg actttacttt atttgtttta tcagaatcac tatataattt
    60421 aggaggtatg tctttgtatt ctttcgacca tatataccat gcagatgtac ccataaaact
    60481 accaatactc ctaaatacaa gattccagcc atttgattta tgttcatcaa aataattatt
    60541 ctctacatca tagtagtaat caacagtgta ttttattttt cttggacttc ccttatgaaa
    60601 ataaaatatt cctccaaatt tatttatctt atataaatta tatcctttag cttcaatatc
    60661 ttctaaccat ttctctatct tatcaggtga actataccag tcaaatttga attttatttt
    60721 cttttccata gcactcattc tccaaaatga tatttttaaa tagcttaaaa atctatttaa
    60781 ataattatat cattttgata ctaattttag gttacaatca tatatttaaa agttatttaa
    60841 atagtaataa actaatagcc ataacaccca ttcctgcaat caatccataa atagataaat
    60901 gatgttcccc gtactctcta gctgatggca ataattcatc tagtgatatg aataccataa
    60961 ttcctcctac aattgcaaat acaattccaa gagttatatc atttaaaaaa tttctaagaa
    61021 gtacatatcc aataatcgct cctaaaggct cagacatacc agataaaaag gaataataaa
    61081 aagctttctt tttatctcct gtggcataat aaactggtac agatacagat ataccttctg
    61141 gtatattgtg tattgcaatt gcaattgcta tcggaatagc tattgtaaca tcatcaagag
    61201 ctgatataaa tgtagctaat ccttcaggaa aattgtgaat tgctatagcc attgctgtaa
    61261 atatacctgt tcttaaaaga gacttatttt ttttgatatt cttttcttct atattctcca
    61321 ttttctttat ttcatgtgga ttttcttctt gtggaattag cttatcaata acagcaatga
    61381 tagccatacc tataaaaaaa gctattacag ttgaccaact tcctaatttc tctcccatag
    61441 attttgtcaa agcatcttgt gctttaggaa aaatttctat catagaaaca taaatcataa
    61501 caccagcaga aaatcctaag cttatagaaa gaaacttagt atttgttttt ttagcaaaaa
    61561 atgctataca acttcctata ccagtagata aaccagccaa taatgttatc aaaaatgcaa
    61621 atattacatt attcaaataa atcaccttct ttttaattga tattgattat cattttcttt
    61681 atgctatcat atatatttta tatagtcaat atttataaaa aatatacaca ataaaaaagc
    61741 agagatataa acttagtctc tgctttttct ttatagtata ttaaaattat ttatttaaga
    61801 aatccactgc atattgcaca tataaagcag ttccaatttc tagagcatct tcatctatat
    61861 taaagcatcc atgatgatga ggataacaag caccttttga ctcatttcta gctcctacaa
    61921 atgctagtgc accaggtgct atattcataa actcagctaa atcttctgct cctgttactt
    61981 tttcaaatag tgttatacag tcttctccaa atagcttaat tgctgcatct gtagctatct
    62041 ttgaacattc tttgtcattt ataactgctg gtgttaagta cccatactct aattcagcat
    62101 ctgctctata tgcttctgct gtagattttg ctatacgttc taaaattcca ggaatttgct
    62161 ttctaagctc tggattaaat agccttattg taccttctaa gatagcttca ctcgcaatta
    62221 cattaaatct agttccagaa tttaaaactc caacactcac aactaatggt tccagaggac
    62281 ttacttctct actaaccata gattgtagat tcataactat tgctgaactt gcaagcactg
    62341 catcaacacc ttgatgagga agtgaaccat gaccacctct accttttaca gtaattttaa
    62401 agaaatcagc agaagccatt cttggtcctt cttctaccga tatggtccca gattctacat
    62461 cagtccaaag atgtattcca aacacactat ctacaccttc catagcacca tcttgaatca
    62521 tagctcttgc acctttacca acttcttctc ctggctgaaa aaataatttt acagttccat
    62581 tgatactatc cttaatatca tttaacactt ttgctgcccc tagaagcatt gatgtgtgac
    62641 catcatgacc acaagcatgc ataagtcctt catttttaga tttgtattct acatcagtgc
    62701 attcaacaac ttgtagagca tccatatcac ccctaagtgc tacagtcttt cctggattat
    62761 ctccttttat agttgcaatt acacctgtac ctccagctga tacatatggt atccctattt
    62821 tatcaagttc ttctttaact ctctttgatg ttctaacttc ttccatactt ttctcaggat
    62881 tttcatgaaa ttcccttcta agcttaatta catagtcttt ataactcttt gttatttctt
    62941 taatatccat tctatacctc caagtagtac acattcttct tatttaaaat actttttatc
    63001 aattaaatac tatattagat ttacaaagat acctgctatt actacagaag ctattgtaac
    63061 tgtagtaaat cctccaacaa gcatctttgg aagcatttta tccattaaaa attctttttc
    63121 ttctggtgtt tcagccaaag cctttactgc ctcttcagtt aatatatagt ttggtggaaa
    63181 accataaagt gatgtaagcg aaactgaaaa agccatttct ttactttcac ctaaaagctt
    63241 acctactata atagagaaaa ttcccatacc tattacacca aatattatga ttcctacaag
    63301 aggaactgcc atttcaacaa gcattgctgg tgtagctttt gctaaccctg caaatataaa
    63361 agccatcaat gatgtcatta accatccaaa tgaaccagat ttattaagtg gttttctatc
    63421 aactattcca agttctgatg ctattacacc aaatattaaa catataacat atcttgatac
    63481 aacttcattt gtaagatttg taaattgtac tgctaaccat gctacaaatc ctaatctaag
    63541 tattatcata tatgtcgtat catatttttc tggtaagtct ggtattatct taaatctgct
    63601 tttttgttca ggtaaatcct taactttttc ttcatctgta tttacagtta cttttccact
    63661 tctaaagtct gataaaagtc tttttccttc ttttttaagc attaatgctg taataggata
    63721 acccgcaaat ccttgtgcaa catacatcaa aattgcaagt actgataaac ttgcaagacc
    63781 ttttgctgta gcagcatctg ccatcattat agatgcaact agccctccag taagaggagg
    63841 tgtagctatt actgctgtat cccatccaaa tactactgtc cctaatgcca aagcacctac
    63901 acatattcct actattcctg ccaaggctat tactattgtt ttccattgac ctgctaattc
    63961 cttaatactc atcatagttc ccatatgagt tattaacaaa agcataccta attgagctaa
    64021 tggtgttcct aaagcggcta agtccacaat attttctggg aaaaaagtcc aaaatcctac
    64081 tataaatata attgctgata caaaaaccga cggaacaaaa gctttagttt ttgctgaaac
    64141 tatatctcct atcgtcagtg atactaaaac tagacaaaaa gctgttattg cctccataca
    64201 aaatcccccc tcataataaa tatattttta taaaatgtat gtatagttat ccattatttt
    64261 actacttttg aaaaacattt caagtcaaaa ttcgattttt ttcataaaat tcgataaatt
    64321 ccataaatta aagttagttt tctaaattat tctttttgta aaaacgttat cccgaccatt
    64381 ggctatttaa ctctattctt acaaagagaa ttaaatgtaa tttgcaaaaa ataaaacata
    64441 ggatacattt tattcataaa acatcttata aatataaaat gaattgtctt tatgaatctt
    64501 aatatatcct atgtttgtaa gtttaatgtg tatgttagct atggtgtaaa atttgtatac
    64561 tagttattgt gtaacctact atatactcca tttttgttta ctagctcttc atgagtacct
    64621 ctttcttcaa ttcctttatc cgtcaatact atgatttcat cagcattttt tatagtagtc
    64681 aatctatgtg caacaactaa atttgttcta tctttactta attcctctaa agactcttga
    64741 atttctcttt ctgttacatt gtctaaagca gatgtagcct catccaatat tattattgga
    64801 ggattcttta aaaagattct tgaaattgaa attctttgtt tttgcccacc tgataatttt
    64861 acgcctcttt ctcctatata agtatcatat ccatctggca aagtctctac aaagtcatgt
    64921 attctagctt ttttagcagc agctatcatt tcttcatctg tagcattagg gttcccacaa
    64981 agaatattat ctcttatagt tcctgtaaat aaaaatacat cttgttgaac tatacctata
    65041 ttttttctca atgattttaa actaacatct tttatactct ttccatcaat ttttatatca
    65101 cccttatcaa attcataaaa tcttggcaat agattacata atgtagtctt tcctcctcca
    65161 gaaggtccaa ctaatgctat agtcttacca gcttttatag ataaacttaa attatccaaa
    65221 acttgagtct tatcttcata tgtgaaagat atattttcta tctcaatgtc accttttaca
    65281 ttttcaagtt caataggatt tttagcttct ttttgatggt cttgttccat tatctctatg
    65341 aatcttttaa aacctgtcat accattttga tattgctctg taaagtttat taattttttc
    65401 ataggttgta taaacatttt tacatatagt atataagcag caaaatcacc caaagtaatc
    65461 tttcctatat aagtaaaata accaccagct attacaacta cccactctaa gatatccatg
    65521 tataaattca taccagagta atattctgcc attaccttat aagaatcttg tcttgctctt
    65581 ctaaataatt tattgcttct tacaaactta tttaactctt ctttctcagt acaaaaagac
    65641 ttagttacct tcatacctgc tatactattt tctaaagtag catttacatc acctatagta
    65701 actctactct tcataaaagc aatattcatc ttatcttttt tagctattgc aaaccaaatt
    65761 ataaacggta atatagcaaa tattataagc gtaagaggaa tatttatatc tataagtata
    65821 aagaatgaac caaccaacat tactatagaa atgaataaat cttcaggccc atgatgtgca
    65881 agttctgtta tatccattaa atcatttact attctagaca tagttactcc agatttgtta
    65941 ttatcaaaat acgtatttgg aagtctttgt aagtgtgtaa atacctcatt tctcatatca
    66001 gcttgcatat ctacaccaat aacatgtccc caatactgca taaaatagtt aagccctgct
    66061 tttgctataa atataagtat aagtgctact gcaaaaacta ctaacattct taagttttta
    66121 tttggaacaa cgtcatccat tatatttcta gttatcatag gataaactaa atcacataag
    66181 gaaaatagaa aagctgctat aaggtctagt gtaaaaattt tcttatatgg cttataatat
    66241 ttgacaaatt gttttatcat aagtattaaa tccctccttt attagtactt ctaaaaaata
    66301 cccataaatt agtataacat aacaataact ttaaatcttt tataaactta caaagtaata
    66361 acaatctttt tcataaaatt gaagatttat atcatatagt tgtatacttg aaacaacata
    66421 aaaagagcta tatctctata tttaaaattt tataaagata caactctata tttaatacta
    66481 aatttttttt ataaatacaa aactacttca atgcatttaa tatcttttta tctaatgctg
    66541 ttgtatttat acctgttata tatcctgttg cctttattgt aggtatatca taagttgttg
    66601 gtagtatagt agtagatacg attaaatcag caccttcttg ttttgttgaa cattcagcaa
    66661 ttttacattg ctcaacttct gcttgtatat tattttcttt aaccaatctt tctactctgt
    66721 cacaaactat tgttgatgtt gcgattcctg ctccacaggc tactaatatt tttttcatat
    66781 attgtcctcc tacaatttct ttaatatttt ataactcaaa ataaaattga taaatttctt
    66841 atatctttat ttatataaat aattatatca ttttatataa gaagaacatc ttaaaattat
    66901 tcttttaata tgttttttat ttcaacattg cttatactta aaatttcatt ttttatacaa
    66961 gactctatgt actttgatat atttttataa tcatttaaat taaactgttc aatattcaat
    67021 ttatattcta tatcactgac aatacctatc aaatcagact tattagataa aatatcctca
    67081 tacttacctt ttctagttac ttccaatctt ttaaatctat aatttttata tccttctatg
    67141 ataatcaagt ccatatcttc atataattga atcattttat ataatggatt cttctctttt
    67201 agttcttcaa tatatgctac tcgtttatct gatattatac agacttttct cgccccagat
    67261 tttctatgtt tataagtatc ttttccttcc tcatccatat caaaatcatg acaggtatgt
    67321 ttaataactc caacacgata ccctttttta tttaattctt ttattatgcc ttcaatcaat
    67381 gtagttttac ctactcctga atatgagaca actgacaata tattatacat aaaattgtgt
    67441 cctttcataa aactatctaa taacaacatc cactaattga ttttctttta ttgtctcatt
    67501 ctttttaatc ttaatcatac aattacttct tagagttgca gataatctag cttttgcatc
    67561 tttcttatca tttagctcta catacaattt accttcactt gtatatagtt ttccacttac
    67621 aaatctatct ttatttgaaa gtttattaaa ttctccttta aatatagctt ttatatgatg
    67681 tgttcccttc ttatatgtta aatactcaat aacaggaact atcacattat caaataccac
    67741 tattgctgca tgtggattac cagaaagacc aaataatagc ttttctccca atgtacctac
    67801 ataacaagct ccaccaggtt taatgtttat cttccaaaat aattctgttg cccctatttt
    67861 tttgaataca tctggaatta aatctttatc tccaacagaa acaccacctg tggtaattaa
    67921 aaaatctaca ctctgtaaca tattctttac ctttttttct aactccaata tatcatcaga
    67981 acaattgcat attgtatctg cttgtattcc aagttttaat aatcttgaat ataaggtata
    68041 tctattacta tcgtatattt taccattctc taaagtctca tttatatctt gtagttctgt
    68101 acctgtattc aaaattccaa cttttaaatc atcataaaca gttactttgg atattccaag
    68161 acttgctaat actccaatac catcatatcc tatattctga tttttattaa taatcaattg
    68221 cccctttttt atatcctcac ccttaaatat gtaattatcg tattccttta attttatatt
    68281 taaagttatc atatcactta taactgttac atcttctttt tttattacac aatttgcacc
    68341 ctgtggtatc tttccaccag tcattatctt cactgcttca tttttattta ataccctatt
    68401 aaatacatct cctgcataca aagtccctat aacctttaaa tttctatttt cagaatttgc
    68461 attatatgca taaccatcat agggtgacct atcaaatgga ggactatcaa ttgttgaata
    68521 tatatcttct gcaactacct tatttactgc atctataaga tttactatct tgaccctgtt
    68581 tattggtaaa acattttttt caataatttt tatggcatct tctaattcta tcattttaaa
    68641 tactccccaa tatatttttt atttaagata tttctatgta atatattata gattataaaa
    68701 gttggaaata cttttatctt atatttaatt ataactaata gttatctata agtgcagttc
    68761 aaattttata aatctaataa aaaaactgtt cccagctaga aatttccttc tatcttgaaa
    68821 cagcatcata tgctttagaa tattttattc taataaagtt ccttttatta tttttacaat
    68881 tttatcactt tcaatagttt ttatagattt tattttgtta tccacaatac ttattgtaag
    68941 tacctctttt tttaatattt tgttatcttc tttcaatcta taactcttaa ctactttact
    69001 gccttcatga ttatatgctg ttaaatcata attcgcatct cctacaattt catttattct
    69061 ggaatcttca acaccaacat actctcccag gtcaatatgt ctaaaatttt ctagtgatac
    69121 agccgatttt tcatggtcac ttttttcaat cactatacta gattcagcat caatgactga
    69181 tataccttgt gatgagaaag aattggtttc aacattgaca actttatttt cgttaatatc
    69241 aacatataaa gcacttccat ctagttcatc atttccagta tattttggat aaactaatgt
    69301 catataactt gatgtattta tatcttgcat tgtatttgat tcttttaaaa ctttaggttt
    69361 tatataataa aatgtattat aaggtatacc catattcttt ataatgtact tataatcttt
    69421 attcataatt tcattaacat cattttgaac ttttgtcata gttttttgct gtgttgcttt
    69481 acttgctact tgatttttct ctttagtatt ttctttatga catccaacaa gtataatacc
    69541 taaaatagtt acacaaacta atgacctttt taacttcata aatcaatcct cctttcttaa
    69601 attttatagt atttatctat atctcaattt acagttatct
//
LOCUS       DAMJYR010000005        64542 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.005, whole genome shotgun sequence.
ACCESSION   DAMJYR010000005 DAMJYR010000000
VERSION     DAMJYR010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 64542)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 64542)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..64542
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.005"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(128..1729)
                     /locus_tag="PC471_000345"
     CDS             complement(128..1729)
                     /locus_tag="PC471_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HDF2787487.1"
                     /translation="MIYDLILKNGFIIDGTGNPGFYGDIAIKDNLIAKIDSKINSNTN
                     KEIDCYGKIITPGFIDPHVHEEIVAILDGKFEKFLKQGVTTTINGNCGHSITPYSSEN
                     VYEYMYKNGLLLEEEKKYLIDKNKCWNNFTEYCDLISKSGISVNMGFLLGHGTIRWSV
                     MGGSKDRPPTEKEKNEITDIINDGMKSGAFGISTGLAYIPSKYADIDELVDIARQIKE
                     HDGIYTSHIRDYIGRYNAVKEAIEVGQKSGARVQVSHLSPVEIEAFDEILKARYNGVE
                     IMVDTVPRSSGHCMKKKRVIQFIMAISSSLFELGIDGVMDALRNEEGRTLILKEAFIL
                     GDRGSIILLNTKDINMEKKSIREIATQKGIDEDKLLLDLLLDGDEELIFCLGGMYRAD
                     FPDKLHDDKIIDNPFVMVGSDCLFSVGGDMSWFELQRRGAFPIFFNMYRKSGVRLEEI
                     VRRVTSLPARQFKIKNRGILKEGLIADIAVIDMDNYSYPRSEDIDFSKPQLLANGVEY
                     VIVNGKIALENGEITENKCGEVLKR"
     gene            complement(1840..2517)
                     /locus_tag="PC471_000346"
     CDS             complement(1840..2517)
                     /locus_tag="PC471_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2787488.1"
                     /translation="MSKVKSIYNEEYLPFMIRYGRLTLSLGIIAALVPGIILSFGFGI
                     MPPISALLASTMAIVSMSAPNYIIEPVSYSPILGIPGTYMSFLSGNISNMRLPCSIAA
                     QKAAEVESGTEEGSIISTIGIAVSILVNISILTIGVILGGSVLSKIPAEVVEKLNLIL
                     PALFGSVFGQVFLQDKKLGLVAIVISVLTIILSKQGIIPQSLVVLICVFGTILIARAM
                     YKDKLSD"
     gene            complement(2530..3225)
                     /locus_tag="PC471_000347"
     CDS             complement(2530..3225)
                     /locus_tag="PC471_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5058 family protein"
                     /protein_id="HDF2787489.1"
                     /translation="MDYLKLANHPLLWIASTVAVSIVVVQSLIFAIKSCKVGKTMGIT
                     DKQIKSAVKSSAISAIGPSMTIFAGMVSLIVTMGGPIAWMRLSFIGSVIFESMSAGFG
                     TDALGITLGSPEMTKLAFTNAVWTMILGSLGWIIFTLLFGHKLDKITNVISSGKKSFI
                     PIISLGAMLGSFAYLNADRVLRFDNGTIACISGMAIMVILCMLEKKKNIKWLREWGLT
                     ISMFSGMIIASVI"
     gene            complement(3277..4476)
                     /locus_tag="PC471_000348"
     CDS             complement(3277..4476)
                     /locus_tag="PC471_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HDF2787490.1"
                     /translation="MKSAYKGYEAYSYLEKGKDISSIEMCKGDKRVKEYLIELDDIQE
                     AKVKSIVDEKIFISLHEHPVLFPEHLERDIFEYNREGKQRCAYEALSRGYYDAIFDNL
                     MDGVCTITSNSGWKWDDILIDLGMRLCDLAHQDFVIKCEKVEDIYRAKSEGKVALIPT
                     LEGAAPIENELDRIDILYGFGIRLMGITYSESNALGSGLKEEKDGGLTNFGKKAVERM
                     NKIGMAIDCSHVGVQTTLDVIEYSKDPIILSHVGARSLWNTKRLTPDEVLIACAKKGG
                     VIGVEAAPHTTITEKNKEHSIESFMEHFEYIKNLVGIDHVAFGPDTVYGDHVKLHSVL
                     AAKFDIKKITGTKEHPKVEYVKGLENPTETSKNIVRYLVKHGYSDEEIEKVLGGNILR
                     VLKQVWK"
     gene            complement(4737..6782)
                     /locus_tag="PC471_000349"
     CDS             complement(4737..6782)
                     /locus_tag="PC471_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDF2787491.1"
                     /translation="MKKHNILFVSTDDKINIDISKQLENIFGEFCNIDNLVYVNRINI
                     ELSSYELVVCSDNDIKEYIHNNIDKNIPIVIVHRTINIENINQIISIENDSDVMVIDA
                     YKESADETAKIIRKLGLIHINLIPYYPGCDKSKCEIGIITGSRNSIPQNIKQIIDIGD
                     KVIDINTVIEIFTKLNISIDKLHIIKEKYNEDTVSGYRYYTTMNKTMKSFLEIIDEGI
                     ASVDKLGKFIYCNKVFSNLVGIDQNEIISNNFMDLFSDKVVKKIFFQEDEVNDEVVNL
                     NNKKLIINKVNVYENNERIKSIISIKNISAIQVLEDKIQNKLQSKGFVSKYTFESVVG
                     ESKIIKEKINIARKIATTDFSVLILGENGTGKEIFAQAIHNESLRKNKPFVAVNLSSL
                     SDTLIESELFGYDEGSFTGAIKGGKMGIFERAHTGTIFLDEIGDISLDVQQRLLRVLQ
                     EKEVMRMGGSKIIPIDVRIIAATNKDLKKKIKEGSFREDLYYRINVLHIEIPRLRERK
                     EDIPLISKYFLDEINSNKCFTEESMQALTLYEWPGNVRELKNLIYYIDTIVEEDRVDY
                     EHLPEQFRFEKNNTLVNENFDSIILDFKQSNFFEESICILTSIETWNNKNILLGRNKL
                     QEILKEKDIVLSVDQIRKRIDKLKSHGLLLSGVKKQGSFITDEGKNFISYVKIKGVI"
     gene            complement(7193..8002)
                     /locus_tag="PC471_000350"
     CDS             complement(7193..8002)
                     /locus_tag="PC471_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDF2787492.1"
                     /translation="MYYVYVNDINIAVYDLNPSAKKTVLFIHGWPLGHKIFEYQTNIL
                     PKLGYRTVSIDLRGFGKSDATSGGYTYSQLADDIYKVVHAIGLKDFTLVGFSMGGAIV
                     LRYMSLFNGYGVSKLVLAAAAAPSFVQRPPEFPYGMTREDVNKLIAQACTDRPEMVTD
                     FGEKVFASNPPESFRRWFNDIGFSASGIGTIGTAVSLRDEELFNDLKCVRVPTGIFHG
                     KLDEICPYEFAVFMNEKIEDSILYTFEYSGHAIFYDELKLFNQEFLQFLEE"
     gene            complement(8086..8619)
                     /locus_tag="PC471_000351"
     CDS             complement(8086..8619)
                     /locus_tag="PC471_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HDF2787493.1"
                     /translation="MSGAHYDMHKRNGYSSDEPYPEIKVLGPNKYYAELLMDDYAGVS
                     SEFTSVNQYLYHNFDLDETHRELSEMWINISITEMLHMEILAKTIRLLGGNPVYRGST
                     SSCGAYWNGGFVCYGNSICNRLKLDLHLEHVAINNYYKDISLIEDPYIKAILNRIILD
                     EKLHVSLFEKAIEKYCK"
     gene            complement(8769..10118)
                     /locus_tag="PC471_000352"
     CDS             complement(8769..10118)
                     /locus_tag="PC471_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HDF2787494.1"
                     /translation="MELKNFKDILFKKALSEGFEECEIYYYTGENLSINIYEGEVEKY
                     NLDKSFGLSFRGKVNGKIGYSYTEILDDKAVDMLIKNVKDGVNTIENEDVQFIYEGDK
                     HYNDVKTYSKELENLEADKLIDLALEMERETKAYSDKVVNLSKCTISYSVSSNGISNT
                     KGLNLSNKTNMLIGFVVPIIEDNGQKYDGIGYNIANSIEEIKPCEIAKLGVDNALSKV
                     GGKSITSGKYKTILLNEAMVSLLSTFSGIFNADSAQKGLSLLKNREGDMIASPIVTIV
                     DDPLLENGMASTPFDDEGVATFKKEVVLNGKLITLLHNLKTANKAGVKTTGNGIKSSY
                     SSPISIYPTNFYIEKGDKSLDEIIKDIDEGLMVTSFAGLHSGANSVTGDFSLAAKGFY
                     IKEGKKVFPVEQITVAGNYFDLLKDIEAIGEDLEFPMSSIGSPSVVIKELSVAGKDE"
     gene            complement(10130..11512)
                     /locus_tag="PC471_000353"
     CDS             complement(10130..11512)
                     /locus_tag="PC471_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011967718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HDF2787495.1"
                     /translation="MLSKNIASKVLEKCLITGGDFAEIFEEDTINNSISLIDNKVENA
                     IGGRNYGIGIRIFKGLKSIYAYTSDNSLNSLLDVAYKAAVALGKLEDGKSVILNDSIK
                     INNIHNIKIYPNSIGNKDKVSVMKVAYKSAKEFSNDISQVSVSYLDKDQKILIANTEG
                     IYVEDRRIRTRLGISSIASKGNENQTGFEGPGGCKGFEIFEEIDPEYYAKESARVAHT
                     MLHAKNCPAGNMMVAIDNGFGGVIFHEACGHSLEATSVAKGNSVFADKLGQQIASTKI
                     TAIDDGTIPNYWGSMNIDDEGNPTQKNILIESGILKSYMIDKLNGRRMGMNPTGSSRR
                     QNYKFAPTSRMTNTYIAAGEDRPEDIIKSIPDGLYAKKMGGGSVNPVTGEFNFAVSEG
                     YLIKNGEIQEPVRGASLIGKGSDILMNIDMVGNNVKQAQGMCGSSSGSIPTNVGQPMI
                     RVKEITVGGR"
     gene            11756..12970
                     /locus_tag="PC471_000354"
     CDS             11756..12970
                     /locus_tag="PC471_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HDF2787496.1"
                     /translation="MNQEYVKGNPIFTNINKVPRQFPYLTDDIDTDVIIVGGGVTGCI
                     CAYYLAKNNIKSVILEKGRIAHGSTSVTTSLLQYELDDNLIDLTEVMTLKDALKAYNL
                     CTSALEELDTFIELYGNKCDYAKRDTLLYTANKLEVKAIKEEYNLRKENGFDVEYIDE
                     STNPFSFDLKSGLIAKNGGRELDPYKYSHHLIDVSLKNGLQVYENTEVKKVDLSNDKV
                     TAEVSYGYKVHGKKLIVATGYNTSLFTKRNFATKSNTFNIATKPLKNIASWKNNILIR
                     DNCDPYNYLRTTKDNRLIIGGEDVSFDDIENETLANEKYDILEQRLKSMFKDIKDIEV
                     EYKYCGCFASTLDNLGFIGPDNKNNNLWYCLGYGANGILFAILGGIMLSELYLGKQNK
                     NMKLFKVDRFDK"
     gene            13147..15060
                     /locus_tag="PC471_000355"
     CDS             13147..15060
                     /locus_tag="PC471_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDF2787497.1"
                     /translation="MKYGGIIMKEILVIAPTKGTYEKSIHIVKKNKYTNIDVVFGNLK
                     EGIPLAEKSINHGTRIIISRGGTYNMLKATYNIPIVEIKVDAYDIIKSYKEVKNSNEP
                     FGIIGFNNVIYGFDIIEEILNKKITMIEIEKEEEIYDAIEKYRKKGINTYIGDTTVAH
                     IVKRLNCKGILIESREENILRAIQQAEQILEATKDEQKRRLQIEAMTDFVHDGIITVD
                     KNFKITLFNKSAEKIFGIKKENALYHNVVDVIPNTVLPQVVETMEPQIGEIQNIGSTK
                     IITNRVPIIVDGQIQGAVSTFQDSKEISSFEHTIRRSLLSKGLSAKYTFDDIIHASEK
                     TKDCIELAKKYACYDTPMIITGESGVGKELFCQSIHNYSKRKNAPFVAVNCAAIPPSL
                     IESEFFGYEEGSFTGARKKGKAGVFELAHEGTIFLDEISEIPLELQGRLLRVLQEKQV
                     MRIGGDKVIPIDVKIICASNKDLKNMMRNGLFRQDLYFRINILTLYLPSLKERKEDII
                     KLSEFFIHKYSSKYNKTPLIITPNIKKALLEREYEGNIRELEGMMERAVIVESFDSIL
                     LEKLSNEYTNNIVDKDNSDFECSNLDLKTLEKKYIYKIYNENNRNTTKTCEILKITRS
                     TLWRKLKEIEDDV"
     gene            15331..16305
                     /locus_tag="PC471_000356"
     CDS             15331..16305
                     /locus_tag="PC471_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate permease"
                     /protein_id="HDF2787498.1"
                     /translation="MHILKSVKKIPGGLMVCPLLLGALFNTVCPQALEIGGFTTSLFK
                     TGAMSILALFCLCNGAQINIRQAGKPLTKGIVLTATKFIIGAVLGIIVSKFIGPKGII
                     GITPLAIVATMTNSNGGLFAALAGEYGDSTDVGAISILSLNDGPFFTMLAFGVTGLAN
                     VPIVALFAALIPILLGFILGNLDEDIREFLAPGTTLLIPFFAFPLGAALNFNQIITAG
                     LPGVVLGLGVTIITGMGGYFVMKLIRAEHPAVGAATGTTAGNAAGTPEALAAIDPTLA
                     TIAAVSTVQVAAAIIVTAICCPMLVSYLDKREKKKKSIFKTNKNIEVN"
     gene            16326..16601
                     /locus_tag="PC471_000357"
     CDS             16326..16601
                     /locus_tag="PC471_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HDF2787499.1"
                     /translation="MNAIIHANEKDNLVTCVRPLIKGEKVSIDGKSYIVNDNIPVFHK
                     MATEDVKKGDVVYKYGEIIGIATVDIKTGDYVHVHNIESTRGRGDKK"
     gene            16614..17777
                     /locus_tag="PC471_000358"
     CDS             16614..17777
                     /locus_tag="PC471_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HDF2787500.1"
                     /translation="MKLMGYLRENGQFGIRNHVLIIPTSVCSSETATRIASLVPGAIA
                     IPHQHGCCQIGSDIELTAKTLIGFGKNPNVAAVLVVGLGCDGIQAKELASEIATTGKK
                     VNYVVIQECGGTLKTVSKGAEIVSKMAREVSKEVRVEFGMSEITLALECGGSDPTSGI
                     ASNPSIGVASNLLVDEGGSSILSETTEVIGAEHLLATRFEDEEMKDKFLKFVSDVEKR
                     AIAMGEDLRSGQPTPGNKAGGLSTIEEKSLGCMYKAGNKPFKGALEYADIVPPDKKGL
                     YFMDTPGQDIDSITGMVAGGAQIVIFSTGRGTPTGSPISPVIKITGNSDTYNKMPDNI
                     DINAGRIITDGAKIADIGQEIFNEIIEVCNGKHTKAESLGHREFGIYRISSTF"
     gene            18114..20393
                     /locus_tag="PC471_000359"
     CDS             18114..20393
                     /locus_tag="PC471_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HDF2787501.1"
                     /translation="MNFENTKNKKIIATLLLTLLLIVSLTCVTFIMNLNKVTHNETER
                     YLVEVSQSISTTINVKCKSIFRTLYSMGDTYFEINDSEKEVNRYLSKKLKLFEFDWLG
                     IIDSNGNFVSALKDTGNVKGQDFYRKALQGKETITPVKSQSGTYGILYSVPLYNGDKI
                     VGAVAAWNTIDTMRNLLSVESFHGEGFSQIIDQDGNFIVSSANKNAPKNVVNFFDMIK
                     HQGKQVDKNKLEEMKINLKKNKTGSMYYTLNDGISKAMHYLPLQEENLYLLSIVPTKT
                     ASAQFDLLLKKAIFINILIVILFLLLIILIMFINQKNRAQLIKIAYVDSVTKGYSKAR
                     FDIEAKKIIQSSSSESYSLVSINIQKFKLINDSFGSEAGDKTLKYIHDVISNYLEPGE
                     LLCRVSNDQFNVLIKTIPQKEILKYIENITNDINSFNKNEECKYFIALSIGVYKIDDP
                     NLPLVSIQDRANVARKNNKGNIGNHPLYTCVFYSDLERLKMIKEKEMENRMEEALNNN
                     EFIVYLQPKISLKDNTIVGAEALVRWQDPNKGLIPPDEFIPFFEKNGFITKLDHYVFE
                     KVCSMIRKWIDNGETPIPVSVNFSRANLNNIDFLEKYRMLRNKYNIPAKLLEIELTET
                     LVFENLQKLINIIDQIHEEGFQCSLDDFGSGYSSLNMLKEIKVDTLKLDRAFFSSPNA
                     DNIAENHVIESIVNLAKKLNMNSISEGVETILQMDYLKKINCDMIQGYVFSKPLPQEI
                     FEKMTFGKKLEETSMDSSV"
     gene            complement(20488..21828)
                     /locus_tag="PC471_000360"
     CDS             complement(20488..21828)
                     /locus_tag="PC471_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HDF2787502.1"
                     /translation="MENQEALRHEKIWILLFRYSIPAIIAMMVTSLYNVVDRAFIGSM
                     EGIGSIAIAGLGVTMPVFTLIIAFGMLVSVGASTRLSIKLGERNREEAEKILGNALTL
                     SIIISLIITILGLVFLEDILFILGASKDSIFYAKDYMSVILVGSIFNLVAFSLNNAIR
                     AEGNPKLAARTMIVGCVLNLILDPIFIFVFNLGIKGAAIATVLCQIVVFFWVIHYFIR
                     GKSNLKLKKCNLKLNKDIVKKVFAIGMTPFAMEVAISITHMFTNNSLKVYGGDLAIGA
                     MTALTSILLMFMMPVFGLNQGMQTIISYNYGAKQYERAKKTLILSIIVSVVILSFGFL
                     VVQVFPEVFVGIFNKDSDLMDIAVRGISLNLITLPIMGISIVGPVYFQCISKVKHSMF
                     LTLLRQFILFIPLIIVLPIQFNLDGVWLAQPVADFIAMIIVLLFLKREFKTSRV"
     gene            complement(21840..22622)
                     /locus_tag="PC471_000361"
     CDS             complement(21840..22622)
                     /locus_tag="PC471_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004460318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2787503.1"
                     /translation="MNCINTKNLNVSYGNTDIIKNLNLNIPKGKITTIIGANGCGKST
                     ILKTIGRIISPNSGKIYINGEDIYKKKSKEIAKDMAVLPQSPQAPSGLTVSELISYGR
                     FPHKSGFGNSSKEDREIVNWSLEVTGIAEFKDRNMEDLSGGQRQRAWIAMALAQETDI
                     LLLDEPTTYLDLAHQLEILKLLDELNKKQGRTIVMVIHELNNAARFADHMIGIKKGKI
                     VCEGDAYDVMTKDNLKELFNIDAEIATDPRTNKPICITYDMV"
     gene            complement(22637..23659)
                     /locus_tag="PC471_000362"
     CDS             complement(22637..23659)
                     /locus_tag="PC471_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HDF2787504.1"
                     /translation="MKNIIKINSKTLLVISILMVLIFITFLLSLNMGSFSIEPMDVIK
                     TFLGQGQRNHEIAIFKLRLPRIVIALLVGSALSTAGVILQGVTKNDLADSGILGINSG
                     AALFVVVYIYLMNGNVYEGMSNLTVFTMPIVALIGALFGAFLIYSLAWKGGINSSRLL
                     LIGIGVNIAFTSILTIFQLKFTTQEFNRVMAWTSGSIWGTSWKYVIAVLPFIVIFMGI
                     TIYKSKYIDVLNLGDEVSISLGVDVEKQRRRLIIYAVILSGVSTAVAGSISFLGLIAP
                     HIGRKIIGPKHKRLIPVSALIGTLLLLIADTISRNLLAPIEIPVGIVVSIIGVPYFIY
                     LMLVND"
     gene            complement(23665..24678)
                     /locus_tag="PC471_000363"
     CDS             complement(23665..24678)
                     /locus_tag="PC471_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HDF2787505.1"
                     /translation="MTKLSKKNITYLLILVSIVVLVFGIVLSITIGAKDMNLSTVIDS
                     LIKMEDGINMRIVKDVRLPRAIAAALVGGFLAVSGAIMQGITRNPIAEPSVIGITQGA
                     TFAISISLVLQKKLPQLIHGSFSVMMFAFIGASISGLFIYFISSKSRGRVNNVKLALA
                     GVALGTLLISLASAISMYFNLSQQLSFWISGGLVGVKWEGIKLLFVAGGIGFILAIIM
                     APRITILSLGEEVAIGLGQKTNFVRFICIVLVILMTGASVSVSGNIIFIGLIVPQIAK
                     GIVGSDYKYIIPSSLVLGAVLLVYTDILSRMINPPYETPVGSITALIGVPIFIYLVRK
                     GEK"
     gene            complement(24806..25744)
                     /locus_tag="PC471_000364"
     CDS             complement(24806..25744)
                     /locus_tag="PC471_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDF2787506.1"
                     /translation="MKKIKSLAIFISIITTLVLVTACSDKNTEDKDKSETRVVQSVKG
                     EVKIPSNPKKIVDISGSSEELLLAGYKPVATANVDSYETDKLPSYIREELKGVKIVGH
                     SMMDTMDMEAILEVNPDLIIMSQRQEKIYDQLKEIAPVVMMKDYANDWRSKLTDVSKL
                     FDKEEEAKSWLQKYDEKATKLGKEVIEKNGEKTYLPVLASSGQFMVFSDGGIGTLIND
                     DMKLAKPKNMPKQDGITLPMVSMEGLTDIDADHIVVIATEADKKDLENSAIWAQIRAV
                     KDGNVTILDAAPFFSQSYNPIGKELLLESVKNELTK"
     gene            26014..26478
                     /locus_tag="PC471_000365"
     CDS             26014..26478
                     /locus_tag="PC471_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016208838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2787507.1"
                     /translation="MVHLVYCDNAGKKGEKVLDKILAGTKTMVIRGAAGRKIPHSRVF
                     EDELLYFMEKGTAKISAKATVKKVENYVKLTEDEISKILNENQPKLNLSEKQKVRWHK
                     KCLCLVEFENVEEITPLDFDHQGNMDDWLIVEKIEDVVVGTSIPYNYEKSKF"
     gene            complement(26590..26907)
                     /locus_tag="PC471_000366"
     CDS             complement(26590..26907)
                     /locus_tag="PC471_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HDF2787508.1"
                     /translation="MDEKMIEISFGIIGFAGDAKGLAFEAIKEAKSGNIDKARECIEK
                     SKESIHKAHKFQTELIQNEANGNKTEISVILIHAQDHLMTAMNFQQLAEEFIDVYERL
                     ESK"
     gene            complement(26920..27651)
                     /gene="chbG"
                     /locus_tag="PC471_000367"
     CDS             complement(26920..27651)
                     /gene="chbG"
                     /locus_tag="PC471_000367"
                     /EC_number="3.5.1.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitin disaccharide deacetylase"
                     /protein_id="HDF2787509.1"
                     /translation="MTKIIINADDFGYCEAVNYGIISAHNNGIVRSTSMMANMPGVEH
                     GVGLLKENKTLNCGVHMTLSCGRPLLSNLKTIVDKDGFFIRRITDEIIEKMDCDEIYR
                     ELCAQIDRVKGLGIDISHLDSHHHIHTLLSLKPVIEKIVTKYNLPIRGGFEYNLEYSK
                     VVPLIDSFYKENVSEEYFIKNIDEIMKYDVVDIMSHPAFLDDYILNSTSYAIDRTKEH
                     KILTSKKVKEFLEKNGLVISSYRDI"
     gene            complement(27677..28987)
                     /locus_tag="PC471_000368"
     CDS             complement(27677..28987)
                     /locus_tag="PC471_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HDF2787510.1"
                     /translation="MKKGLKIVTIGGGSSYTPELVEGFINRYEELPVKELWLVDIEAG
                     KEKLEIVGNLAKRMVKKAGVDMKINLTLDRREALRDADFVTTQLRVGLLDARIKDETI
                     PLSHGVMGQETNGAGGLFKALRTIPVIFDIIKDVEELCPNAWMVNFTNPTGIITEAVF
                     KYTNFRRYIGLCNVPIHLKNDVAKLFNVESDRISMDFAGLNHMVYGLNVSLDGEDVTK
                     EAIDKFVKADISMQNIKAIDFNAEFVKSLGAIPCPYHRYYYKTKEMLEDELREFKEGK
                     ARGQVVKELEEQLFELYKDEKLDVKPPQLEKRGGAYYSDAACNLISSIYNDKKDIQVV
                     NTLNKGSIRDFKDEQAVEVSSVITKNGPKPISIGYLPDSVHGLVSQIKSFEVLAAKAA
                     VYGDYESALLALCINPLIPSDDLAKTILDEMLEAHKDYLPRFNR"
     gene            complement(29084..30397)
                     /locus_tag="PC471_000369"
     CDS             complement(29084..30397)
                     /locus_tag="PC471_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDF2787511.1"
                     /translation="MEKFMSFMDKYIVPVAAKIGAQRHLVAVRDAFIVMIPITMVGAL
                     GTLINNLPLEAYKNLMASIFGENWTTFGGDLWWGAIGTMAVFLVIGVAYFLAKSYESD
                     GLQSGLIALSIFFIMAPQIGKIVPEGGTTVVEGWGMIQQTYLGTAALFSSILIGLLST
                     EIFVRLSKVKKLTIKMPDGVPPAVSRSFAKLIPGMLTIMIFTVIGIFIKMLSNGSFLT
                     DILNTYLGAPLSNVADSLGSTMLIAFIIHILWTVGLHGANIALPFTETILMKLGGENA
                     ALAQAGATEGYHVLAGAFFDAFVYLGGSGMVLGLIVALLIAGRRRKEMIVLGGPPAIF
                     NIGEPLIFGLPIVLNPIFMIPFVLAPVICSAVSYLAIDFGLVAPVILPKIPWVTPPIL
                     GGAMATGDWTGGALALFNLILSILIYIPFVIASEKMEANKLKINN"
     gene            complement(30419..30730)
                     /locus_tag="PC471_000370"
     CDS             complement(30419..30730)
                     /locus_tag="PC471_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDF2787512.1"
                     /translation="MIKIMLACSAGMSTSLLVTKMEDAAKENGIEAKIWAISEVNLKN
                     EIENCDVLLLGPQVRYVLGKATEMAKPYNIPVEVINMMDYGRCNGKAVLDRAVELNSS
                     K"
     gene            complement(30930..31634)
                     /locus_tag="PC471_000371"
     CDS             complement(30930..31634)
                     /locus_tag="PC471_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDF2787513.1"
                     /translation="MKEPIYKVIENHVRELINSDSLKEGDLIPSEKQLSEEFNVTRMT
                     VRSALNNLVKEGYITRQRGVGSIVIANNIYDNISSVSGFTKEMESKGYKVSNILVSLE
                     IVQADENLSSKLNISLEENVWEIKRVRLANDVRVSYMITYMPVKLFPNLNKTHCENSL
                     YNFVEEVCGYKIAMSEREVQAVISNEECMDNLKLEEPEPLLYISQICKLQNSEIFEYS
                     HTYHYGYTLTLNAVVE"
     gene            complement(31725..33086)
                     /locus_tag="PC471_000372"
     CDS             complement(31725..33086)
                     /locus_tag="PC471_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HDF2787514.1"
                     /translation="MKDRLNLTEGRITEKLLKLSLPIMGTSFIQMGYNMIDMIWVGKA
                     GSKAVAAVGTAGFFPWLAMAFIMISKVGGEIKVAQSIGANNISTTKSYMKSAIEINII
                     LAIIYTISLIVLNKQLIGFFRLGDLEVITMSRQYLIIVALGMVFYFINPVFTAIFNGL
                     GNSKTPFRINTVGLITNIILDPILIFGWGFAPKLGVAGAAIATVFAQIVVTTCFIYII
                     VKSKEEYFKLRLFKEIETKYYKVLYKLGLPIAIQSGMFTVFSMLLGVIVASWGPVAVA
                     VQKVGSQIEAISWMTAEGLSVALGSFVGQNYGAKKYSRINKGCKIAITVSSVLGIITT
                     LVLVFAGESIFSIFIDESEAIEKGAVYLKILGYSQFFMCLEIITTGAFKGLGRTYIPS
                     IIITILTGARVPLAYFISRPEMLGLNGVWWSITLSSILKGILMISLFISLLKLGKLYK
                     ISE"
     gene            33585..34739
                     /locus_tag="PC471_000373"
     CDS             33585..34739
                     /locus_tag="PC471_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HDF2787515.1"
                     /translation="MKINKKFNRVDMQVSILTAIIVIISSSCVYFFNYTLTYKSMIYS
                     LSERSKHIYEYVERNIDKNTFTEINSKEDQSKKSYKSSKILLESVKNTTGVMYLYTAK
                     KTKDGSLVYVVDGLNSSRSDFRNAGDLIEKEIWGDLNKALDNHILLPKKIKSTSWGYI
                     FISYFPIHNDVGKVVGVIGIEFEAKHQYNTFKFISIVTPIIALLTCLISALIAVILFK
                     RISNPTYTDFANTDYLTGLKNRNAFEIDMNNLNNSNLKNLISIISIDLDGLKKINDKF
                     GHQTGDLYIKQASKIIQYVINKKYIVYRIGGDEYIIILKNLSHKEINNIINNINLKIV
                     EENSTNELKLSMSIGYAIFDEKVDVSLFDTYRRADSQMYDNKRKINKQYQ"
     gene            complement(34821..36539)
                     /locus_tag="PC471_000374"
     CDS             complement(34821..36539)
                     /locus_tag="PC471_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     permease subunit"
                     /protein_id="HDF2787516.1"
                     /translation="MNNNAISSKSISLKSKMNLDIKKCMLTVLVMSLVIFLLAPIIYL
                     FVQAFLDKDGVFVGLQNFQEYLASESLLNSLKNTVFVSVISTVISLTLALVYAYALTR
                     TKIKFKGFFKSLAMFPMFAPTMMHAIGLVYLFGNQGLVTNLLGVHVPIYGAVGIILAQ
                     IIYVFPQSFQILYISLSSTDYRLYEASNTMGISKIREFISITIPSIKYSLISSAFVSF
                     TLCFTDFGAPKVIGGEFNVLSIDVYKQIIGQQNMPMGATVSILLLIPAIISFIVTKKI
                     EKNQNSYITSKSIPYKVKTNKLRDSILGGVVGIIAFGIIAVMLTVILSSLISVWPYDL
                     RLTLEHFSMKNSIDGIKPFINSIKMSLLTAILGTSFVFLFAYLNEKTEKAPYLKSFGY
                     FLSTLPMALPGLVLGIAYVLFFNKLEFNFMDTIYIKNLFNGLYGTLLIMVICNIVHFF
                     SVAFITATTAIKKVDKELDMVAKSMGINSASILKNVTIPLCLPAVLENFMYFFLNSMV
                     TISALVFIYTASSKVAAISIVQLDDKGSTAQAASLAVLILVTNILVKVIYEFIKNRLE
                     KRTNFN"
     gene            complement(36544..37533)
                     /locus_tag="PC471_000375"
     CDS             complement(36544..37533)
                     /locus_tag="PC471_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDF2787517.1"
                     /translation="MSYLKINNVFKSYDQKRVLNNISLDIEEGEFLCLLGPSGCGKTT
                     LLRIIAGLEDVNSGTIILQDKDITNLEPSKRGFGIVFQSYALFPNMTAYNNIAFPLKE
                     RKVSKGKIDNKVKEVLETVGLTNEAHKYPKALSGGQQQRIAIARALALEPKFLLLDEP
                     MSALDAKVRHKLRMDIKRLQKELNITTIMVTHDQEEAITMADKIAILNGGDIMQIGTP
                     EEIYQNPQNLFTAQFIGDTNCFDNGDSILTVRPEYVQIEKSTKENYQGIISNIEFRGN
                     LLRVEIKDKLNENFIISDASIKEWVNLNLVEGDLVKISIDEKYYLKYPKVKGA"
     gene            complement(37629..38678)
                     /locus_tag="PC471_000376"
     CDS             complement(37629..38678)
                     /locus_tag="PC471_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HDF2787518.1"
                     /translation="MKEFIFTCLCIVVITFMTSCISTTPQTTQGKGNNKLTIYAALEE
                     EQIAYYMEGFREEYPNIDVEIVMDSHGVIASKLLAEKDNPQADIIWGLSAMNMIELKK
                     DGALEAYKPKNYDKVDKKFKDTEDDVSWVGMSVPETAIVVNYKELKKLGIDIPKSYED
                     LIKPEYKGLITMPNPASSGTGYLTVSGLIQMMGEKDAYNYMDKLHKNIATYTHSGSKP
                     AKLAGSGEYPIGITLGYRATQQLSSGEPIEVVFPKEGSGWDLEANALVKKDNIKKEAK
                     LFLDWAISDSAMDRYAHEVAVTSIKTDNPVPKGYSKQPFEQLIKEVDLNSAAKNRSTV
                     LKTWESKYGSKSESK"
     gene            complement(38955..39623)
                     /locus_tag="PC471_000377"
     CDS             complement(38955..39623)
                     /locus_tag="PC471_000377"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HDF2787519.1"
                     /translation="MARLNINPTRMEMTRLKKLLKTATRGHKLLKDKLDELMKQFLEI
                     VRENKRLREEAENALDTAYKNFIIARAVMSQEYLGSALMMPKQSVSVDVSTRNIMSVD
                     VPVFDFKTENNQSDIYPYGLAFTSGELDSAMEAFSDAMQPLLRLAESEKSAQLLAQEI
                     EKTRRRVNALENVMIPNYIETIKYIAMKLEENERASTTRLMKVKDMVLKKALEEKKKN
                     DLVV"
     gene            complement(39629..41002)
                     /locus_tag="PC471_000378"
     CDS             complement(39629..41002)
                     /locus_tag="PC471_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HDF2787520.1"
                     /translation="MIKEYKSIQEVAGPLMLINGVEGVKFDELGEIELSNGEIRRCKV
                     LEVNGDTALVQLFESSTGINLAESKVRFLGKSLDFGVSPDILGRVFSGMGRPIDGGPE
                     IIPDKRLDINGAPINPAARDYPAEFIQTGVSAIDGLNTLVRGQKLPIFSGSGLPHANL
                     AVQIARQAKVRGTDSKFAVVFAAVGITFEDAEFFISDFKATGAIDRSVVFVNLANDPA
                     VERVSTPRMALTAAEYLAFEQDMHVLVIITDITNYAEALREVSAAKKEVPGRRGYPGY
                     LYTDLATMYERAGKMKGHEGSITMIPILTMPEDDKTHPIPDLTGYITEGQIILSRELY
                     KKDIQPPIDVLPSLSRLKDKGIGKGKTREDHADTMNQLFAAYSRGKDAKELMAILGES
                     ALSEIDLMYAKFADEFEKEYVSQGFRTDRTIEQTLEIGWKLLSMLPKSELKRIRDEYF
                     EKYMPKE"
     gene            complement(40999..42777)
                     /locus_tag="PC471_000379"
     CDS             complement(40999..42777)
                     /locus_tag="PC471_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HDF2787521.1"
                     /translation="MKTGTIVKVSGPLVVAEGMRDANMFDVVRVSDKHLIGEIIEMHG
                     DKASIQVYEETSGLGPGEEVVSLGMPMSVELGPGLISTIYDGIQRPLEKMYDISGTNI
                     TKGVEVSSLDRTTKWEFKPKKSVGDKVVAGDIIGGVQETAVVECKIMVPYGVKGTIKE
                     IYSGEFTVEDTVCVITDEKGNDIPVTMMQKWPVRKERPYREKKTPDSLLVTGQRVIDT
                     FFPITKGGVAAIPGPFGSGKTVTQHQLAKWADADIVVYIGCGERGNEMTDVLLEFPEL
                     KDPRTGESLMQRTVLIANTSDMPVAAREASIYTGITIAEYFRDMGYSVALMADSTSRW
                     AEALREMSGRLEEMPGEEGYPAYLGSRLAQFYERAGKVNCLGMDEREGTLTAIGAVSP
                     PGGDTSEPVSQATLRIVKVFWGLDASLAYKRHFPAINWLTSYSLYQEKVDVWADKNVN
                     DNFSAQRAQAMKLLQVESELQEIVQLVGVDSLSDGDRLILEVTRSIREDFLHQNSFHE
                     VDTYTSLHKQYRMMGLILKFYKSAQDAIKQNVTINDIIKLSVLEKIGRAKYVEEGDID
                     AAYDSIEDEIDNELADLIKKRGAEEL"
     gene            complement(42894..43217)
                     /locus_tag="PC471_000380"
     CDS             complement(42894..43217)
                     /locus_tag="PC471_000380"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HDF2787522.1"
                     /translation="MLMYKVGVVGDKDSIMGFLALGIDIFPAYDSDEIKKSIHKLVED
                     EYAIIYITEQASLLAKESIAKYKDYQLPAIIVIPGIGGSMGLGMNEVRESSKRAIGAD
                     ILFKE"
     gene            complement(43204..44181)
                     /locus_tag="PC471_000381"
     CDS             complement(43204..44181)
                     /locus_tag="PC471_000381"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit C"
                     /protein_id="HDF2787523.1"
                     /translation="MAEEKTYPYAVARIRVLEKQLLNRQMFIQMAEAKSPEDSLRIIA
                     EAGYADTSNNNIHNFENILTQELSKTYEILKSLAPEEKFVDVFLYKNDYHNLKVLIKE
                     EISGVDGEKYLIDGGTIPLIKLRESLANRSFSDLPKIMSSAILEAFDVYNKTQNGQMV
                     DIVLDKATFTSMKETAKESQNKFVIDYVMKVCDLTNLKSFIRVKNMKKDFDMFMNVFV
                     SGGSLDKEKFLEAFSSDTPASCFKSTSYSDVCKNGMDSGFTVFEKLCDDYLMEYVRGA
                     KFKPLTLEPLIAYLYAKESEIKTIRIILTSKLNNIDADTIKERLRDAYV"
     gene            complement(44196..44759)
                     /locus_tag="PC471_000382"
     CDS             complement(44196..44759)
                     /locus_tag="PC471_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit E"
                     /protein_id="HDF2787524.1"
                     /translation="MGNEQKMIDRIIADAKQEAQEILDKAKSEADLKVNSANEKAEKE
                     MASYTKLAEAEAEKAASKEISGAYMEAKKQILSKKQEILEEVILEAKNKLLNLKDNEY
                     EEIILNMIEKSNCTDDSEIVLSKKDKKTLKDVLSKKGIKVSDETRDITGGFIVKKGDI
                     EYNYSFEAIIAVEHEYIEQIAAEILFN"
     gene            complement(44811..45302)
                     /locus_tag="PC471_000383"
     CDS             complement(44811..45302)
                     /locus_tag="PC471_000383"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HDF2787525.1"
                     /translation="MEFLNALGNGQFLAISGAAIAALFAGMGSAKGTSIAGQAAAGVV
                     TEDPSKFGQLLVLQLLPGTQGLYGFIIAFLILSKAGIIGGDAIPTSAQGLQLLMAGLP
                     IGLVGLVSGIAQGKAAAAGVGIVAKRPEELGKAITFAAIVETYALLGLLVSFLAYNGI
                     AIG"
     gene            complement(45305..47230)
                     /locus_tag="PC471_000384"
     CDS             complement(45305..47230)
                     /locus_tag="PC471_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HDF2787526.1"
                     /translation="MAIVNMNKISIVGLEAQKSQILKLLMNRGFVQIDDSAFLTEEEE
                     LKNYLVKDSEESEVIMLEQKMSHVNQAINIVSSLVKQKKSLLAPKREFNKIDSEKEGQ
                     VYAEALELNNLHKEIGTIRSNINLLNNNKSMLEPWVNFDIPLENMETKYTKTILGTVP
                     ISANIDALRVKLEDEIPESVIGIVNSDKLMNYVYLITLKETFDDVLEVLKEFNFSVVS
                     LPNEEGTPLQAIKKYNEMIIRQENVSKDKTEEIKRHADSIHMLENLYDFYTIEREQKK
                     IIERLVKTKTTFCLNGWLPSKRADELIKEITEKFNCCVQTEEGSKEEGFPILLENNKL
                     VTPFESVTNMYSYPSTKDIDPNTILTFFFVVFFGMMLSDAAYGLIIAVVCGFVVYKLK
                     IQKGEGNLIKLIGICGVSTTVWGLIFGGILGDLIPIKALINPLEDVMTLMGMSLLFGI
                     IHIYVGLGIKAYTLIRSGKVIDAIFDIGFWYLCITGVCLLIIPFVAGDIGVFSEVGKY
                     LAIIGAIGLVLTQGRSFKGIPVKLFKGFSSLYGITSYFADILSYTRIMALCLTTGVIA
                     QVINLLGAIAGPILAVVIGVVGHTINLLINALGAYVHTSRLQYVEFFNKFYEGGGVPF
                     VPFKYKTKYTSINKKEM"
     gene            complement(47232..47543)
                     /locus_tag="PC471_000385"
     CDS             complement(47232..47543)
                     /locus_tag="PC471_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2787527.1"
                     /translation="MAFEIIKSIVEAEQTADSIKVKAVTDAESIRADAVNKCESIFAD
                     VKKQAKLMEETLIEKAVTDSRAEVDKILANAKSECLKIEKTAEERKSKAIEAVIGKVV
                     R"
     gene            complement(48173..48448)
                     /locus_tag="PC471_000386"
     CDS             complement(48173..48448)
                     /locus_tag="PC471_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif11-like leader peptide family natural product
                     precursor"
                     /protein_id="HDF2787528.1"
                     /translation="MNEKLTKILEEAKNDDEFRKELIKTREQRDVMDSFCKVVTSRGY
                     DMTVGELFSMGEEYTSNLLKSVNGGAAYPIEDWSDWYEDFFIMLSNI"
     gene            complement(48563..49972)
                     /locus_tag="PC471_000387"
     CDS             complement(48563..49972)
                     /locus_tag="PC471_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase/peptidoglycan binding
                     protein"
                     /protein_id="HDF2787529.1"
                     /translation="MIDGKEVKQESGQKNLKFKIAIVVGVLFVAYIGMAIFFRNHFYF
                     GTTINGVKASGKTVEQVNDLLTSSTNSYTLNLEERNNKKEQIKAKDIDLKFTPDDKVQ
                     KIKDSQSAIGWIGGIFKSKEYNNMITLSYNKDLLEKRFNALSCFDSKNVVEPKSAYPK
                     YDSTKNAYVIVPEVLGNKVKKDDFYKLVQNSINSGNTSISLDESKVYEAPNNTKDSEK
                     LKQAIKTLDKYVGVVVTYDFSDRKETLDGSEINKWLKVDSEKNYDITFDNDAMVEYTR
                     GLSKKYSTFGDSRPFKTATGKDITISGGIYGWLIDKKKEAEALVDVVKAGKNVTREPI
                     YIQKAVSRNKDDIGNTYVEISLSGQHMWFFKNGEMLVSTDVVTGDLARGFATPSGIYP
                     LNYKARNVSLTGQGYSSPVSYWLPFNGNIGIHDATWRNSFGGSIYKSSGSHGCVNTPF
                     SKAKKIYENIEPGTPIILY"
     gene            complement(50343..51005)
                     /locus_tag="PC471_000388"
     CDS             complement(50343..51005)
                     /locus_tag="PC471_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
                     /protein_id="HDF2787530.1"
                     /translation="MIDISIDDKLKERWPNAKLGCIQAKVAVNKSSEKLINEINEFCD
                     TINQSLKVEDITKLDKIKDARNAYKELGKNPSRYRTSSEALVRRIIQGKGLYTINNIV
                     EINNLISIKSLYPVGTYNVSKLHSPVCFTVGDEGEQYKGIGKELINIENLPILSDSVG
                     KFGSPTSDSERAMITEDANEILICIFSFSQESDIEEYFGYAKELLKEYADGKDIETKI
                     IK"
     gene            complement(51259..53478)
                     /locus_tag="PC471_000389"
     CDS             complement(51259..53478)
                     /locus_tag="PC471_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HDF2787531.1"
                     /translation="MTGRNKSFTLITILFLTSLFLTMGYIYIEDTKHLLMSEAKIHIK
                     EVAVQGSQLAQRQIEKDLDVLNIFSNYYASNPDIPNEEKMKNLLDEMENQKFYTMAIV
                     DINGNAENTKGDKFSVKDREFFKNSIKGKKYVSSPYVDEVNKSIKKIAISVPLLNNDK
                     VVGVLYCTYNINTLMKLINISFYENNSISYVVKNNGTIILHPQGDSLSKNIYKLLKQD
                     NDIQEVNRLKKELQENKTGATVLNMLEERRYLGYATMDNGNSENNYIKDWNLIFSIPE
                     TVVFSNSKQIINRAVYAVLSIVLIFIAIIFYIIYIKKSNEKEILSLAYEDKVTYIGNQ
                     NKFYRECSKYLLDKPSLNYIIVYFDINNFKMINDTFGYEFGDNLLITIAKALKEELTE
                     GEVYARLSSDYFAIFCDYKNGRNKIIRKLDNIRSNIESNLSIVFEISLCVGIYFVEEG
                     EVDIQKAVNKANMARSVAKGKNINYAIYNEDVRNKLSEESMILDDIKIALVKNQFEVY
                     YQPKFSLVTGEMIGSEALIRWNHPEHGFISPAVFIPIAEKSKLILKIGRFVFERVCND
                     LYEWKKQGKKIVPVSVNLSRVELYQPDIVKFINKTIKMYNLSSDFIEIEITETVAINE
                     LNILKNVLNELRTYGFSISMDDFGTGYSSISCLRDMPIDILKLDKSFLGGIEHDERSR
                     NIAKSIVSLAKSLDLVVIIEGVESKEQAELMKQFGCDLVQGFYFARPMPAKNFLDLL"
     gene            complement(53754..56408)
                     /gene="adhE"
                     /locus_tag="PC471_000390"
                     /gene_synonym="adhC"
     CDS             complement(53754..56408)
                     /gene="adhE"
                     /locus_tag="PC471_000390"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HDF2787532.1"
                     /translation="MAHKEQVIIENKKENEVMSVGLDTVNNVESLVKKLAKIREAQKI
                     FATYSQEQVDKIFLAASLAANKQRVPLAIMAQKETGMGIAEDKVIKNHYASEYIYNAY
                     KETKTCGVIEKDEAFGMTKIAEPIGVIAAVVPTTNPTSTAIFKALLALKTRNGIIFSP
                     HPRAKNSTIEAAKVVLEAAVLAGAPEGIIGWIDEPSLELTTTVMKEVDLTLATGGPGM
                     VKSAYSSGKPAIGVGAGNTPAIIDDSADIKTAVNSILVSKTFDNGMICASEQSVIVLE
                     NIYNEVKKEFKERGAYLLDKDETEKIRNIILVNGGLNSKIVGQTACTIAKLAGFEVPV
                     DTKVLIGEVESVEIEEAFAHEKLSPVLAMYKASDFDDAVRKAEKLVEDGGFGHTSSLY
                     IDDVNQREKLDKFTSAMKTCRILINTPSSQGGIGDLYNFKLAPSLTLGCGSWGGNSVS
                     ENVGVKHLLNIKTVAERRENMLWFRAPEKVYFKKGCLGVALKELKDVMNKKRAFIVTD
                     TFLYNNGYTKAVTDLLDEMNIKYTTFFEVEPDPTLECAKIGAKAMREFNPDVIISIGG
                     GSAMDAGKIMWTLYEHPDVDFQDLAMRFMDIRKRVYTFPKMGEKANFVAIPTSAGTGS
                     EVTPFAVITDQDTGVKYPLADYELMPNMAIVDSDMMMNMPKSLTSASGIDALTHALEA
                     YVSMLATDYTNGLALQAIKSIFEYLPRAYDNGAKDPEAREKMANASTMAGMAFANAFL
                     GVCHSMAHKLGAFHHVPHGVANALLITEVMKFNSSDAPKKMGAFSQYKYPEALKRYAE
                     IASFLGLKGNSDEEKFQSLLVAIEDLKLKVGIPKSIKEFGVEESKFMDSIDEMVIQAF
                     DDQCTGANPRYPLMNEIKDMYLNSYYGR"
     gene            56912..57112
                     /locus_tag="PC471_000391"
     CDS             56912..57112
                     /locus_tag="PC471_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2787533.1"
                     /translation="MEKLKTISVFSLIISVILTIGGIGIVTYYVDNLFIRGLSVFVLI
                     MSSSFVSTTVRLIFEESKRYKF"
     gene            complement(57319..57909)
                     /locus_tag="PC471_000392"
     CDS             complement(57319..57909)
                     /locus_tag="PC471_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipicolinate synthase subunit B"
                     /protein_id="HDF2787534.1"
                     /translation="MRLEGLTIGVGFTGSFCTYDKIFIELENLVKEGANVHTIFSDVS
                     QNIDCRFGNSEEFMKKAYELTGNKPIVTIEEAEPFGPKGIADIIIIAPCTGNTAAKLA
                     NGITDSPVLMAAKGHLRNDKPLVISISTNDALSFNFKNIGILLNSKNIYFVPFGQDNC
                     KAKPNSMIAHTELIIPTIELALENKQLQPVIKSPHQ"
     gene            complement(57912..58787)
                     /gene="dpsA"
                     /locus_tag="PC471_000393"
     CDS             complement(57912..58787)
                     /gene="dpsA"
                     /locus_tag="PC471_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipicolinate synthase subunit DpsA"
                     /protein_id="HDF2787535.1"
                     /translation="MSNQYDITVIGGDLRQVYMVKKLINKGYSVVTYGISNEIINGIV
                     GKSTSLAEALSKSSIILCPIPFTKNQVNIENTDDCLDATIDNLLINLSPNQILFGGNI
                     PTKVCKFCEQHSIQIYDFMKMNDVAILNAVATAEGAIAEAIKRSIINIHQSNCLVLGY
                     GRCAQILAKKLYALDANVCVGARNNDARSTANAFGYSSISLSQLDEKLLQFDYIFNTI
                     PECILTKERLEKVSQEVTIIDIASSPGGVDYSVVKEAGINATLCLGLPGKYSPKTSGE
                     ILVNAIENIINERRD"
     gene            complement(58863..60254)
                     /locus_tag="PC471_000394"
     CDS             complement(58863..60254)
                     /locus_tag="PC471_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HDF2787536.1"
                     /translation="MNYYELLRIKKERHKNKNFLIIDGEKYTYENILSYSEELGKQIS
                     LGENIPILIYSKNIMFQLISFFAINYSKNIPIICHYNLSKKVLDDILLKNNISIIISD
                     ESMDVIDLYDDNNNEVAKKVNFSIFNPKFNIYMYQYKKLINYLDKSICMGALSSGSTS
                     VPKVLYRTYESWAGFFPIQNGIFNISGESILFVNGTLSFTGNLNSIMSVLYEGGSIVI
                     SSGLNCKSWIRTIKQYNVTNIYLIPTKLQLLVKHLKEPVLKVVSIFTGSQLLFEDTAR
                     NLKKYMPKSEIILYYGASELNYITYLCYDELIEKPLSVGRPFPGIDIYIREGKIFVNT
                     EYAVYNATKPYSVNDIGYYDNDGYLIFEGRSDDIINIGGFKVSSTKIENEVKKIPQIE
                     DAIVLPYSDTIRGNQIVLFVITIDKITKKDLLIKMKDNLMKNEIPKKIIFLNSFPYTS
                     SEKIDRLALLKML"
     gene            complement(60258..61394)
                     /locus_tag="PC471_000395"
     CDS             complement(60258..61394)
                     /locus_tag="PC471_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HDF2787537.1"
                     /translation="MKEVFILGGLRSHIGLKNGIFQFVQPELLGASVLKDLIKKYEID
                     KIDEIICGNAVGTGGNIARLMTLTAGVSNEVPAFTVDMQCASAMMSIDIAFSKVKSGQ
                     CDLIIAGGFESSSLQPMRTYHKNDKRYNTNNPNYTVAQFSPDDNSQNSMLEGAERVAE
                     LYQIEKADLDFWVKESHKRAKEAREEKILEDIISPINNSTYDEGIRDKMSQRLLDRMP
                     SILGKETITNAANSCLINDGASFIIICSKKYLEHVKKKPKAKIINTCTIGTDATLSPT
                     SAIQAMDKLLEIESLNYLDVSAVEFNEAFAVIDVLFQRKYPELIDRYNIFGGALAYGH
                     PYGASGAIIALHLLKALEKTKGRYGICSIAAAGGLGSALLLERV"
     gene            complement(61426..61971)
                     /locus_tag="PC471_000396"
     CDS             complement(61426..61971)
                     /locus_tag="PC471_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HDF2787538.1"
                     /translation="MKNTKVNIQDLTKMSICVALLCISSYIYIPLPFTPAGVTAQTIM
                     INLIALILTPRQAFSTVGVYIMIGLIGIPVFGGGTSGIGKLLSPTGGFYFGFLFAALI
                     ISLLKGRNNDIKRYILITIFVGMPIIYFMGTVFMCYFNQMTVEAALFAAVVPFLIGDT
                     IKSVIASFLGTKLNNVLRRNL"
     gene            62295..63065
                     /locus_tag="PC471_000397"
     CDS             62295..63065
                     /locus_tag="PC471_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2787539.1"
                     /translation="MKVNENNIDEELDELSRKVYLESPDFWISISDKIIDKVFDEMLL
                     FFAVKYNYFNIVCFAIENNYLDLNMPSKNKKYTNIMEHLLDTSKQNEDKKIYNYLLSL
                     NKGNNLDERPILDELNCNISSDTFEKKDIYLPKYLCPSCNSNIFDTGYKVSNDITYKF
                     SSKEAEPIEMCSEVSSIRCCNCDELLEDITLDKLYNLCKIQNCNNCGSNLTKVGIIAK
                     KKMNFDENTEKFKDVSTFYCCGNCDSEISLSQREHFKI"
     gene            complement(63519..63947)
                     /locus_tag="PC471_000398"
     CDS             complement(63519..63947)
                     /locus_tag="PC471_000398"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HDF2787540.1"
                     /translation="MIKKLNSKDINKIMEIWEKSTIKAHDFISKEYWQNNYNTVKNEY
                     IPISDTFVYDDGDEIKGFISIIDKNFIGALFIKPKYQNLGIGGKLLDYATKKYKSLSL
                     AVYKDNKKAVVFYNKKGFNIVKEQVNEDSGFKEYIMEYSK"
     gene            complement(64143..64376)
                     /locus_tag="PC471_000399"
     CDS             complement(64143..64376)
                     /locus_tag="PC471_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HDF2787541.1"
                     /translation="MMILIQSRGKMKCKELSEELEVSERQIKSYKMYLEQAGIFINST
                     PGIYGGYEIDKCNSISLIKLLDSEVSIFISTKW"
BASE COUNT        21553 a        11080 c         7689 g        24220 t
ORIGIN      
        1 attattaaaa taaatattaa tactataaat aaaaacgaag catgattttt atttaaataa
       61 atctcaaact tcgtctttac ttacttagat atttatttac tttctaaatc ctattatttt
      121 taaaacctta tctttttaat acttcaccac atttattctc agtaatctct ccattttcta
      181 gcgcaatttt tccatttaca attacatatt caacaccatt ggctagtaat tgtggctttg
      241 aaaaatcaat atcttctgac ctaggataac tatagttatc catgtcaata actgctatat
      301 cagctatcaa cccttcttta agaatacctc tattttttat tttaaattgc ctagctggta
      361 atgaagtaac tcttcttaca atctcttcca atctaacacc agactttctg tacatattga
      421 agaaaattgg aaaagctcct cttctttgta gttcaaacca tgacatatcc ccacccacac
      481 taaacaaaca atctgaacca accataacaa atggattatc aattatttta tcatcatgta
      541 gtttatctgg gaaatcagct ctatacattc ctcctaaaca aaagataagt tcttcgtctc
      601 catctaaaag taaatcgagt aataacttgt cctcatctat tcctttttgt gtagctattt
      661 cacgtattga tttcttttcc atattaatat ctttagtgtt tagaagtatt atactccccc
      721 tatctccaag tataaatgct tcttttaata taagtgttct tccctcttca tttctaagag
      781 catccattac cccatctata cctaattcaa atagactaga tgatattgcc ataataaatt
      841 gaattactct ttttttcttc atacaatgcc cagaacttct tggtactgtg tcaaccatta
      901 tttcaacacc attataccta gcctttaata tctcatcaaa tgcttctatt tcaactggac
      961 ttaagtgaga tacttgaacc ctagcaccac ttttctgccc aacctctatg gcttctttta
     1021 ctgcattata tcttcctatg taatctctaa tatgagatgt atatatacca tcatgctctt
     1081 taatctgcct tgcaatatca acaagttcat caatatcagc atacttactt ggtatatatg
     1141 ccaatccagt agatattcca aaagcaccac ttttcattcc atcatttatt atatctgtaa
     1201 tttcattttt ttccttttct gtaggaggtc tatccttaga acctcccata acactccatc
     1261 ttatagtccc atgccccaac aagaatccca tattgacaga aataccactt ttagatataa
     1321 ggtcacaata ttctgtaaaa ttattccaac atttattttt atctataaga tacttttttt
     1381 cttcttcaag taatagacca tttttataca tatattcata tacattttca gaagaatatg
     1441 gtgttataga atgtccacaa ttaccattta tagttgttgt aactccttgt tttagaaact
     1501 tttcaaattt accatctagt attgctacta tttcttcatg aacatgaggg tcaataaatc
     1561 caggtgttat aatttttcca taacaatcaa tctctttatt tgtattgcta tttatcttag
     1621 aatctatttt tgctattaag ttgtctttaa ttgctatatc tccataaaaa ccaggattac
     1681 ctgtaccatc tattataaat ccatttttta agattaaatc atatatcaaa atattccctc
     1741 cttaaaaacc aatttttaaa taatcttaat gtaattttta tatttgaaaa tcaaattatt
     1801 aaatttcata tttgaaaatt cactattatt tcattgtaat tagtcactaa gcttatcttt
     1861 atacatagct cttgcaatta atatagtacc aaatacacat ataagaacta ctagagactg
     1921 tggtattata ccttgctttg ataatataat agtcaataca gatattacaa ttgctactag
     1981 acctaatttt ttatcttgta aaaatacttg tccaaaaact gacccaaata gagctggtaa
     2041 aattaagttt aatttttcta ctacttcagc tggaatttta gatagtacgc ttcctcctaa
     2101 aattacccct atagttagta tacttatatt cactaaaata gatacagcta ttcctattgt
     2161 agatattatc gagccttctt ctgtacctga ttcaacttct gctgcctttt gagctgctat
     2221 tgaacatggt agcctcatat tagatatatt accagaaaga aaactcatat aagttcctgg
     2281 gatacctaag attggtgaat aagaaactgg ttctattatg taatttggag cactcatact
     2341 gacaattgcc attgtacttg ctaataatgc tgatattgga ggcataatac caaatccaaa
     2401 gctcaaaatt attccaggaa ctaatgctgc tataataccc aaagatagtg ttagtcttcc
     2461 ataccttatc ataaaaggta aatattcttc attgtatata gattttactt tactcataaa
     2521 actcccccct tatattactg aagctattat cattccgcta aacattgata ttgtaagccc
     2581 ccactctctt aaccatttta tatttttctt cttttcaagc atacacaata ttaccattat
     2641 agccattcca cttatacatg caattgttcc attatcaaaa cgcaacaccc tatcagcatt
     2701 tagatatgca aatgaaccca acattgctcc tagagatatt attggtataa atgatttctt
     2761 gccactagat ataacattgg ttatcttatc tagtttatgt ccaaatagta atgtgaatat
     2821 tatccatcct aaagaaccta atatcatagt ccaaactgca tttgtaaatg caagttttgt
     2881 catttcagga gaacctaaag ttatacctaa tgcatctgtt ccaaatccgg ctgacattga
     2941 ttcaaaaatt acagaaccta taaatgataa tctcatccat gctataggcc cacccatagt
     3001 tactataagc gataccattc cagcaaatat agtcattgaa ggcccaatag ctgaaatagc
     3061 actactctta acagctgact ttatttgttt atctgttatt cccattgttt taccaacttt
     3121 gcaagatttt atagcaaata taagggattg taccactact attgatactg ctactgtact
     3181 cgctatccac aaaagtggat gattagctaa ttttaaataa tccatttata aaacctccct
     3241 ataaatcctc aaattttatt aatatttcta atatatctat ttccaaacct gttttagtac
     3301 tctaagtata tttccaccta gtaccttctc tatttcctca tcactatatc catgctttac
     3361 taaatatcta acaatgtttt ttgaagtttc tgttgggttc tcaagacctt ttacatattc
     3421 tactttggga tgttcttttg ttcctgttat tttttttata tcaaatttag cagctagtac
     3481 tgagtgtaat tttacatggt cgccatatac tgtatctggt ccaaaagcta catggtctat
     3541 tcctactaaa tttttaatat actcaaaatg ttccataaat gactctattg aatgttcttt
     3601 attcttctct gttattgtag tatgaggtgc tgcttctaca cctataaccc ctcctttttt
     3661 agcacatgct attaatactt catctggagt tagtctcttt gtgttccata aacttcttgc
     3721 cccaacatgg cttaatatta ttgggtcctt gctatattct attacatcaa gtgtagtttg
     3781 aactccaacg tgagagcaat ctattgccat tccaatttta ttcattcttt caacagcttt
     3841 ttttccgaaa tttgtaagac ctccatcttt ctcttctttt agtcctgaac ctaatgcatt
     3901 ggattcgctg tatgttatac ccattaacct tataccaaat ccatacagta tatctattct
     3961 atcaagttca ttttctattg gtgctgcacc ttctaatgtt ggtatcaatg ctactttacc
     4021 ttcactttta gctctgtata tatcttctac tttttcacac tttataacaa aatcttgatg
     4081 agctaaatca catagtctca tgcctaaatc tattaatata tcatcccatt tccaccctga
     4141 atttgatgtt atcgtacata caccatccat taaattatca aatatagcat cataatatcc
     4201 tcttgataat gcttcataag cacatctttg tttaccttcc ctattatatt caaatatatc
     4261 tctttctagg tgttctggaa ataatactgg atgttcatgt aatgatataa atattttttc
     4321 atccactata cttttaacct tagcttcttg aatatcatct aattcaataa ggtattcttt
     4381 aactctttta tctcctttac acatctcaat agatgaaata tcttttcctt tttctaaata
     4441 actataggct tcatatcctt tgtaagctga cttcataaca acatcctcca agttctacaa
     4501 ttttttatat ttttcctatg tagcaattga tgtgccaaaa tataaatttt aattgtataa
     4561 taaatcttgc aaaaaataaa aacagtagta aggttgatat ttactactgt tttattaaaa
     4621 ataatcttct tagattattt attcaatatt tttgaattat cttaatctta aagataattt
     4681 tatttatttc tatccattat agggattttt ttagggtgtt ttgccttttt agtcccctat
     4741 atcactccct taatttttac atatgatata aagttctttc cttcatcagt tataaaacta
     4801 ccttgttttt ttactccaga taaaagtaaa ccatggctct ttaacttatc tattcttttt
     4861 ctaatttggt ctacacttaa aactatatct ttttctttta aaatctcttg cagtttattt
     4921 cttcctaata atatattttt attattccaa gtttctatac tggtcaatat acatatactt
     4981 tcctcaaaga aattagattg tttgaaatct aagataattg aatcgaaatt ttcattaact
     5041 aaagtattgt ttttttcaaa tctaaattgt tctggcaaat gctcgtaatc tactctatct
     5101 tcctcaacta tagtatcaat ataataaatt aaatttttaa gctctcttac atttcctggc
     5161 cattcataca atgttaaagc ttgcattgat tcctctgtaa aacatttgtt gctatttatt
     5221 tcatctaaaa aatacttact tataagaggt atatcttcct tacgttctct aagtctaggt
     5281 atctctatat gtaatacatt aatcctataa tacaaatctt ctctaaaaga accttcttta
     5341 atttttttct ttaaatcttt atttgttgca gctataattc taacatctat tggtataatt
     5401 ttacttccgc ccattctcat aacttctttt tcctgtaata ctcttagcaa tctctgctgt
     5461 acatctaaag atatatcacc tatctcatct aaaaaaattg ttcctgtatg tgctctttca
     5521 aatataccca tttttccacc ttttattgct cctgtaaaac ttccttcatc atagccaaaa
     5581 agttcacttt caataagtgt atctgacaaa gaagatagat taactgcaac aaatggcttg
     5641 ttttttctta gagattcatt atgtattgcc tgggcaaata tttcttttcc tgttccattt
     5701 tctccaagta tcaacacaga aaagtctgta gttgctattt ttcttgcaat attaattttt
     5761 tcttttatta ttttactctc acctacaaca ctctcaaatg tatactttga tacaaaacct
     5821 tttgactgaa gtttattttg aatcttgtcc tcaagcactt gaattgcact tatattttta
     5881 atgcttataa tactctttat tcgttcatta ttttcataaa catttacttt attaattatc
     5941 agttttttgt tatttaaatt tactacttca tcattaactt catcttcttg aaaaaatatt
     6001 tttttgacaa ccttatcact aaataagtcc ataaagttat tagatataat ttcattttgg
     6061 tctattccaa ctagattaga aaatactttg ttgcaatata taaattttcc taatttatca
     6121 acagaagcaa taccttcatc tataatctcc aaaaaactct tcatggtttt attcatagtt
     6181 gtataatacc tatatccact tactgtatct tcattgtatt tttccttaat aatatgtagt
     6241 ttatctatag atatatttag ttttgtaaag atttctataa ctgtatttat atcaattacc
     6301 ttatcaccta tgtcaattat ttgctttatg ttttgtggta tgctgtttct gcttcccgta
     6361 ataattccaa tctcacattt agacttatca caacctggat aatatggtat tagattaata
     6421 tgaattaatc ctagttttct aattatcttg gcagtttcat ctgctgactc tttataagca
     6481 tctataacca taacatcaga atcattttct atacttatta tctgatttat attttcaata
     6541 tttatagtac gatgtactat tacaataggt atatttttat caatattatt atgtatatat
     6601 tcctttatat cattgtctga acatactaca agctcatagc ttgacagttc aatatttatc
     6661 ctatttacat ataccaagtt atctatatta caaaattctc caaatatatt ttccaattgt
     6721 tttgatatat ctatatttat tttgtcatca gttgatacaa ataaaatatt atgttttttc
     6781 atagtcccac ctcctgttta acgagtatag aaagtaataa tttggtctat tgtagcactt
     6841 tttaaaaaag ttttaaatct taaaaatcaa attttgtacc atttactatt attttccata
     6901 ataaaataat gtaagctatc ttttttcaca tataatttaa tttctaaaac ggttagtttg
     6961 acattattgg aatataaata aaaatttaat ataaattcgt taatatatta atacattttt
     7021 aagaatgaca aggaataaat tataacaaat tttaggaata tataataata gggtctcaga
     7081 acttagttac aagttctaaa accctatttg tttagtgtaa aaaacatcat aaaatttagc
     7141 ttaaatcttt tcacataata ttactctttt tctaagtgat aataaaatat atctattcct
     7201 ctaaaaactg taaaaattcc tgattaaata gtttaagctc atcatagaat atagcatgac
     7261 cactatactc aaaagtatat aaaattgaat cttctatttt ttcattcata aaaactgcaa
     7321 attcatacgg acatatttca tctaatttcc catgaaatat accagtaggc actcttacac
     7381 attttaaatc attaaataat tcttcatctc ttaaagatac agcagttcca atagtgccaa
     7441 tacctgatgc actaaaacca atatcattaa accatcttct aaagctttct ggtggatttg
     7501 atgcaaatac tttttcgcca aaatcggtta ccatttctgg tctatcagta catgcttgtg
     7561 ctatcaattt atttacatct tcacgtgtca taccatatgg aaattctgga ggtctttgta
     7621 caaatgatgg tgcagcagct gcagctaata ccaattttga cactccatat ccattaaata
     7681 aactcatata cctaagtacg attgctcctc ccattgaaaa accaacaagg gtaaagtctt
     7741 ttaatccaat agcatgtacc accttataaa tatcgtctgc taattggcta taagtatatc
     7801 ctcctgatgt tgcatcagat ttaccaaaac ctcttaaatc aattgaaact gttctatatc
     7861 cgagttttgg caatatattt gtttgatatt caaatatctt atgtcctaag ggccaaccat
     7921 gtataaaaag tacagttttt ttagcactcg gattcaaatc atatactgct atgtttatat
     7981 cattcacata tacataatac atatcaacag caactccctt caagaaaatg tatgatggtt
     8041 attttatata tatgaataaa atgatgaata actaaatttt caaatttatt tacaatactt
     8101 ctctattgcc ttttcaaata aacttacatg aagtttttca tctaggatta ttctatttaa
     8161 tatagctttt atataagggt cttcaataag acttatatct ttataataat tattaatagc
     8221 cacatgctcc aaatgcaaat ctagttttaa tctgttacaa atagaatttc cataacatac
     8281 aaaaccacca ttccaatatg caccacaaga acttgtagaa cctctatata caggatttcc
     8341 acccaataat cttatagtct ttgctaatat ctccatatgt aacatttctg ttatagatat
     8401 atttatccac atttcactta actctctatg cgtttcatct aaatcaaaat tatggtatag
     8461 atattgattt acactcgtaa attcacttga tactccagca tagtcatcca tcaataattc
     8521 tgcataatac ttatttggac ctaaaacttt tatctctgga tatggctcat cacttgaata
     8581 tccatttcgc ttgtgcatgt cataatgtgc accactcata atatctcctc ctttaaattt
     8641 aatgtatgat attaattgta tgcatcacaa ctttttcata taacttagat aataaaatct
     8701 acttaaataa taaaaagcca tattaattaa aattaacatg accttttatc cacaaaatta
     8761 ctttttaatt attcatcttt tccagctact gatagctctt taataacaac tgaaggtgaa
     8821 cctatactac tcattggaaa ttctaaatct tctcctatag cttcaatatc tttcaacagg
     8881 tcaaaatagt ttcctgctac tgtaatttgc tccactggaa atactttttt tccttctttg
     8941 atataaaagc ccttggcagc taaagaaaag tctcctgtta ctgaatttgc acctgaatga
     9001 agtcctgcaa agctagtaac catcaatcct tcatctatat cctttataat ttcatctaaa
     9061 gatttatctc ctttctctat ataaaaatta gttggatata tgcttatagg tgatgaatat
     9121 gaagatttta ttccatttcc tgtagtcttt acacctgcct tattagccgt ttttaagtta
     9181 tgaagtaaag ttataagctt tccatttaaa acaacttctt ttttaaatgt agctactcct
     9241 tcatcatcaa aaggagtaga tgccatacca ttttctaaaa gtgggtcatc aactattgta
     9301 actataggag atgctatcat gtctccttct ctatttttca ataaagataa tcctttttga
     9361 gctgaatctg cattaaatat cccagaaaat gtgctaagca aagataccat agcttcatta
     9421 agcaatatag ttttatattt acctgatgta atactcttac cacctacttt agataaagca
     9481 ttatcaacac caagttttgc tatctcacaa ggttttattt cttcaataga atttgctata
     9541 ttatatccta ttccatcata tttttgtcca ttgtcttcta tgataggaac tacaaaccct
     9601 ataagcatat tagttttatt gcttaaatta agacctttag tattagatat accattactt
     9661 gatacagaat aagatatagt acatttagat aaatttacca ccttatctga ataagcttta
     9721 gtctctcttt ccatttctaa agctaaatca atcaacttat ctgcctctaa attttctagc
     9781 tctttagaat aagtctttac atcattatag tgcttatctc cttcatatat aaattgtaca
     9841 tcttcatttt caatagtgtt tactccatct tttacatttt ttatcaacat atcaacagct
     9901 ttatcatcta aaatttctgt ataagaataa cctatctttc catttacctt acctctaaaa
     9961 gataaaccaa aagatttatc aagattgtat ttctcaactt ctccttcata tatatttata
    10021 cttaaatttt ctcctgtata ataatatatt tcacactctt caaaaccttc acttagagcc
    10081 tttttaaata atatatcttt aaaattttta agttccacaa ttatccctcc tatcttccac
    10141 ctacagtaat ttcttttacc cttatcatag gttggcctac attagttggt atacttcctg
    10201 atgatgagcc acacatcccc tgagcttgtt ttacgttatt cccaaccata tctatattca
    10261 taagtatatc gctacctttt ccaattaaac tagcacctct aactggctct tgtatttctc
    10321 cattttttat taagtaacct tctgaaactg caaaattaaa ctctcctgtt acaggattta
    10381 cagaaccacc acccattttt ttagcataaa gtccatctgg tattgacttt ataatatctt
    10441 ctggtctatc ttctccagca gcaatataag tgttagtcat ccttgaagtt ggtgcaaatt
    10501 tgtagttttg tctacgagaa cttcctgtag gattcattcc cattcgtcta ccattaagtt
    10561 tatctatcat ataagacttc aaaattccac tttcaatcag tatattcttt tgagttggat
    10621 ttccctcatc atctatattc atagaacccc agtagtttgg tattgtacca tcatctatag
    10681 ctgtaatctt tgtggaagca atctgttgac ctaatttatc tgcaaataca gagttcccct
    10741 ttgcaactga tgtagcttcc aaagaatgtc cacaagcttc atgaaatatt actccaccaa
    10801 atccattatc tattgctacc atcatattac ctgcaggaca atttttagca tgcaacatag
    10861 tatgcgctac tctagctgat tcttttgcat aatattctgg gtctatttct tcaaatattt
    10921 caaacccttt acaacctcca ggaccttcaa atccagtttg attttcatta cccttagatg
    10981 caattgaact tatccctagt ctagttctta ttcttctatc ctcaacatat ataccttcag
    11041 tgtttgcaat taatattttt tggtctttat ccaaataact gacacttact tgagatatat
    11101 cattactaaa ttcttttgca ctcttatatg caaccttcat aacacttact ttatctttat
    11161 ttcctattga atttggataa atttttatat tatgtatgtt atttatttta atactgtcat
    11221 ttaatattac actttttcca tcttccaatt tacctaaagc aacagcagct ttgtatgcta
    11281 catccaacag actatttaat gaattgtcac ttgtataagc atatatactt ttcaatcctt
    11341 taaatattct tattcctata ccataatttc ttccacctat tgcattttct actttattat
    11401 ctattaaact aatagaatta tttatagtgt cttcttcaaa aatctcagca aaatctcctc
    11461 cagtaattag acatttttct aataccttag aagctatatt ttttgaaagc ataaatttac
    11521 ctcctacttt aaatttttaa ttaactttta ttcaatcttt ttacttgtta acaaacatta
    11581 tatactatat tacttaaaat aaggtaatta tgaatatatt atacatatgt tttatactaa
    11641 aatgttatca cattttatct atacataccc atataactag ataattaaaa tgtataatat
    11701 tatttgctat agaaaataat acactcatac ataataaatt ttaggagtga agtatatgaa
    11761 tcaagaatat gtaaaaggaa atcctatttt tactaatatt aataaagttc caagacaatt
    11821 tccatacctt actgatgata ttgatactga tgttataatt gttggtggtg gcgtaactgg
    11881 atgtatttgt gcttactatc tagctaaaaa caatataaag tctgtcattc ttgaaaaagg
    11941 aaggattgca catggaagca ctagcgtgac aacttctcta ttacagtatg aattagatga
    12001 caatttgata gatttaacag aggttatgac tctaaaagat gctctaaaag catacaacct
    12061 ttgtacttca gcacttgaag aattagatac atttatagag ctatatggaa ataagtgtga
    12121 ttatgccaaa agagatactc tactttatac tgccaacaaa cttgaagtta aggctatcaa
    12181 agaagaatat aacttaagaa aagaaaatgg atttgatgtt gaatatattg atgaatccac
    12241 aaaccctttt agctttgatt taaaatcagg attgatagca aagaatggag gacgagaatt
    12301 agacccatat aaatatagtc accatttgat tgatgtaagt ttaaaaaatg gtcttcaagt
    12361 atatgaaaat acagaagtaa aaaaagtaga tttatcaaat gataaggtta ctgctgaagt
    12421 atcgtatgga tacaaagttc atggtaaaaa attaattgta gctacagggt acaatacaag
    12481 tttatttaca aaaagaaatt ttgcaactaa gtcaaatact tttaatatag caacgaaacc
    12541 acttaaaaac atagcttctt ggaaaaacaa cattttaatt agagataact gtgacccata
    12601 taattatcta agaactacta aagacaatcg attaattata ggtggtgaag atgtaagttt
    12661 tgatgatata gaaaatgaaa ctttagccaa tgaaaagtat gatattttag aacaaagatt
    12721 gaaaagtatg tttaaggata taaaagacat tgaagttgaa tataaatatt gtggttgttt
    12781 tgcttcaact ttagataatt taggttttat tggtccagat aataaaaata acaatctatg
    12841 gtattgctta ggttatggag caaatggtat actctttgca atattaggag gtataatgtt
    12901 aagtgaatta tatcttggca aacaaaataa aaatatgaaa ttatttaaag tagatagatt
    12961 tgataaatag atattatata ttaaaagact gtaaaaagat gaaactttat aattctaaaa
    13021 gttaactttg atacagtctt ttataatgtt ccttttaaaa atactttatt tataattaaa
    13081 tataaagtaa aatgttaaaa tataactaat aaaatttagt cataagataa tatttaagtt
    13141 tatttgatga aatatggagg aattattatg aaggaaatac tcgttattgc tcccactaaa
    13201 ggaacctatg aaaaatcaat tcacatagtt aaaaaaaaca aatatacaaa tattgatgtt
    13261 gtatttggca atctaaaaga aggtatacct cttgctgaaa aaagtattaa tcatggtact
    13321 agaattatca taagccgagg tggaacttac aatatgttaa aagctactta caatatacct
    13381 atagttgaaa ttaaagtaga tgcttatgat attataaaaa gctacaagga ggtaaaaaat
    13441 tccaacgaac catttggaat aattggattt aataatgtca tctacggttt cgatattata
    13501 gaagaaatat tgaataagaa aataactatg attgaaattg aaaaagaaga agaaatttat
    13561 gatgctatag aaaagtacag aaagaaaggt ataaacacat atataggaga taccactgtt
    13621 gcacatattg ttaagagact taattgtaag ggaatattaa tcgaatctag agaagaaaac
    13681 atacttagag ccattcaaca agcagaacaa atactcgaag caaccaagga tgaacaaaaa
    13741 agaagattac agatagaagc tatgacagat tttgtacatg atggaataat aacagttgat
    13801 aaaaatttta aaataacatt atttaataag agtgctgaaa aaatatttgg tataaaaaaa
    13861 gaaaatgcac tttatcataa tgttgtagat gttatcccaa acacagttct tccacaagta
    13921 gttgagacta tggaacctca aattggtgaa atacaaaata tagggagtac aaaaattatt
    13981 acaaatagag ttccaataat tgttgatggt cagattcaag gggctgtttc aacatttcaa
    14041 gatagcaagg agataagttc ttttgaacat acaataagaa ggagcttatt aagcaaaggt
    14101 ctatctgcta aatacacttt tgatgatata attcatgcta gtgaaaagac aaaagattgt
    14161 atagaacttg caaaaaaata tgcttgttat gatactccta tgattattac tggagaatct
    14221 ggagttggta aagaactatt ttgtcaaagt atacataact atagtaagcg taaaaatgca
    14281 ccttttgttg ctgtaaattg tgctgccata cctccatctc taatagagag tgagtttttt
    14341 ggttatgagg aaggttcatt tacaggtgct agaaaaaaag gtaaagcagg tgtattcgaa
    14401 ctagcacatg aaggcacaat cttcctagat gaaataagtg aaatccctct tgaacttcaa
    14461 ggtcgattat tgagggtatt gcaggaaaaa caagttatga gaattggtgg tgataaagtt
    14521 attcctatag atgtaaaaat tatctgtgct tcaaataaag acttaaaaaa tatgatgcgc
    14581 aatggtttat ttagacaaga cctttatttt agaataaata tactaacatt atatcttcct
    14641 tcattaaaag aaagaaaaga agatattata aaattatcag aattttttat acataaatat
    14701 tcaagcaaat acaataaaac gcctttaatt atcactccaa atattaaaaa ggcacttctt
    14761 gaacgagaat atgaaggtaa tataagggaa ttggaaggta tgatggaaag ggctgttatt
    14821 gtggaatcat ttgactcgat tttattagaa aagcttagca atgaatatac aaataatata
    14881 gttgataaag ataatagtga ttttgaatgc agtaatttag atttaaaaac tttagaaaaa
    14941 aaatatatct ataaaattta taacgaaaat aatagaaata ctacaaaaac ctgtgaaatt
    15001 ctaaagataa ctcgctccac tctatggcgt aagttaaaag aaatagaaga tgatgtttaa
    15061 aaataataca tatctatata tatcaaaacg ttttatgttt aattttaata cataagacgt
    15121 ttatttgttt tcaataaaaa atacaattat atgctaaaaa gaatctttgt aagtgaaatt
    15181 ctcgtcaatc atacttttaa ttataagtaa tctacagcat ttcaatcgat tattaaaaat
    15241 aatcatgata actaaagata caaactctat atattggcat agtaattgct tttataatac
    15301 ctatataaaa atgaaaggga gtgatactag atgcacatct taaaatcagt gaaaaagata
    15361 ccaggaggac ttatggtctg tcccttgtta ttgggagcac tatttaatac agtatgtcca
    15421 caagctcttg aaattggagg attcacaaca tccttattta aaactggtgc aatgagtata
    15481 cttgctctat tttgtctatg caatggagct caaatcaata tcagacaagc tggtaaacca
    15541 ttgactaaag gtatagtact tacagcaaca aaatttataa ttggtgcagt attaggtatt
    15601 attgttagta aatttattgg acctaaaggt ataattggta ttacacctct tgccatagtt
    15661 gctaccatga ctaattcaaa tggaggtcta tttgctgctc ttgcaggtga atatggagac
    15721 tccacagatg ttggagcaat atctatctta tctctaaatg atggtccttt ttttacaatg
    15781 cttgcttttg gagtaactgg acttgcaaat gtacctatag tagcactatt tgcagcactt
    15841 ataccaatac tacttggatt tatacttggt aaccttgatg aagatatacg agaatttctt
    15901 gcacctggta caacattatt aattcctttc ttcgcttttc ctttaggagc agcattaaac
    15961 tttaatcaaa ttataactgc tggactacct ggagttgtac ttggtttagg agttactata
    16021 ataacaggta tgggtgggta ctttgtaatg aaacttatac gtgcagaaca tccagcagta
    16081 ggtgcagcta caggtacaac agccggaaat gcagctggta ctcctgaagc tttagcagct
    16141 atagacccta ctctagctac aatagcagct gtttcaacag tacaagttgc tgctgctatt
    16201 attgttacag caatatgttg tccaatgtta gtttcctatc tagataaaag agaaaaaaag
    16261 aaaaaatcta tttttaaaac aaataaaaat attgaagtaa attaatactt aaggaggtaa
    16321 aattaatgaa tgcaattatt catgcaaatg aaaaagataa tctagttaca tgtgtcagac
    16381 ctttaattaa aggtgaaaaa gtttcaattg atggaaagtc ttacatagta aatgataata
    16441 tacctgtctt tcataagatg gctacagaag atgtaaaaaa aggtgatgtt gtatacaaat
    16501 acggtgaaat tataggaatt gcaacagttg acataaaaac tggtgactat gttcatgtac
    16561 ataacattga aagtacacgt ggtcgtggag ataaaaaata ggaggaagtt aaaatgaaat
    16621 taatgggtta tttaagagaa aatggtcagt ttggtattcg taatcatgtt ttaattattc
    16681 caacatctgt atgttcaagt gagacagcta caagaatagc atcacttgtg cctggagcaa
    16741 tagcaatccc tcatcaacat ggatgttgtc aaattggttc tgatatagag cttacagcaa
    16801 agacattaat aggatttgga aaaaatccta atgttgccgc agttttagta gttggtcttg
    16861 gatgtgatgg tattcaagca aaagaattag catctgaaat agctacaaca ggaaaaaagg
    16921 taaattacgt tgttatacaa gaatgtggtg gaacattaaa aacagtttct aagggagctg
    16981 aaatagtaag taaaatggct cgtgaagtat caaaagaagt aagagttgaa tttggtatga
    17041 gtgaaattac attagcactt gaatgtggtg gttctgaccc aacaagtgga atagcttcaa
    17101 acccttcaat aggcgttgca tcaaaccttc ttgtagatga aggtggaagt agcatactaa
    17161 gtgaaactac agaagtaatt ggtgcagaac atttgttggc tacccgtttt gaagatgaag
    17221 aaatgaaaga taagtttctt aaatttgtaa gtgatgtaga aaaaagagca attgctatgg
    17281 gagaagattt acgtagtggt caacctactc caggcaataa agctggtgga ctttcaacaa
    17341 ttgaagaaaa gtcacttggg tgtatgtata aggcaggcaa taagccattc aaaggtgctc
    17401 ttgaatatgc tgatattgta cctcctgata aaaaaggatt atattttatg gatactccag
    17461 gtcaagatat agattctatc actggtatgg tagcaggtgg tgcacaaatt gtaatctttt
    17521 caactggtag aggtactcca acaggaagcc ctatttcacc agttattaaa atcactggga
    17581 atagtgatac atacaacaaa atgccagaca atatcgatat aaatgcaggt agaattataa
    17641 cagatggtgc taaaatagca gatataggtc aagaaatatt taatgaaata atagaagtct
    17701 gtaatggtaa acataccaaa gctgaaagtc ttggtcatag agaatttggc atttacagaa
    17761 tttcatctac attttagatt gtaacaaaat atcttttata aatataatac ttatgtagat
    17821 acataagcta aaagttcttt ctaatactat aaaaattttc tatactgaaa aggaactgta
    17881 tcaaaattat tttgaaaaca gttccttttt taacctcttt tactcaaaat attataaaat
    17941 aagatttaaa caataaacgt atcttgatta atctctaaga agacataaat aaagctaaat
    18001 gtaaagtggg ataaataaaa tttaatatct ttgtatgaaa agatatttgg tataatatgt
    18061 ctgagagaat tcttatttgt aaacaaataa aggatggagg atgaaacaaa agaatgaact
    18121 ttgaaaacac aaaaaataaa aaaataatag ctactttact attgacactc cttttaatcg
    18181 tgtcattaac atgtgtcact tttattatga atctaaataa agtaacacat aatgaaacag
    18241 aacgatatct tgtagaagtt tcacaaagta tttccactac aattaatgtg aaatgtaaaa
    18301 gtatatttag gacactatat tctatgggag atacttattt tgagattaat gatagtgaaa
    18361 aagaagtcaa tagatactta tcaaaaaaat taaaattatt tgaatttgat tggttaggta
    18421 ttatagattc taatggaaat tttgtaagtg cccttaaaga tacaggtaat gttaaaggac
    18481 aagactttta tagaaaagcc cttcaaggca aagaaacaat tactcctgtt aaatcacaat
    18541 ctggtacata tggaatttta tattctgtac cactatacaa tggtgataaa attgttggag
    18601 ctgttgctgc ttggaatact attgatacta tgagaaattt attaagtgta gaaagctttc
    18661 atggtgaagg attttcccaa attatcgacc aagatggaaa ttttattgta agttcagcaa
    18721 acaaaaatgc tccaaaaaat gtggtaaatt tttttgacat gattaaacac caaggaaaac
    18781 aggttgataa aaataaactt gaagaaatga aaataaactt aaaaaagaac aaaactggtt
    18841 ctatgtatta tacattaaat gatggtattt ctaaggcaat gcattatctt ccattacaag
    18901 aagaaaactt atacctcctt tcaattgttc caacaaaaac agcaagtgca cagtttgatt
    18961 tacttctaaa aaaagcaatc ttcatcaata ttttaattgt tatactattt ttattgctta
    19021 ttatattgat tatgtttata aatcaaaaga acagggcaca acttataaaa atcgcctatg
    19081 tagactctgt aactaaaggt tacagtaaag cacgttttga tattgaagct aaaaaaatta
    19141 tacagtcatc ttcatcagaa agctattcat tggtatctat aaatattcag aaattcaaac
    19201 ttattaatga ttcttttggt agtgaagcag gagataaaac attaaagtac atacatgatg
    19261 tcattagtaa ttatctagaa ccaggagaat tgctttgtcg agtttctaat gaccagttta
    19321 atgtacttat taaaactatt cctcaaaaag aaattcttaa gtacatagaa aatatcacca
    19381 atgatattaa tagtttcaat aaaaatgaag aatgtaaata ttttatagct ctatctatag
    19441 gtgtatataa aattgatgac ccaaatctac ctttagtttc aattcaagac cgtgcaaatg
    19501 ttgcacgaaa aaataataaa ggaaacattg gaaatcatcc actttataca tgtgtttttt
    19561 atagtgattt agagcgttta aaaatgatta aagaaaaaga aatggaaaat agaatggagg
    19621 aagcattaaa taataacgaa tttatagtat atcttcagcc taaaataagc ctcaaagata
    19681 atactattgt aggtgcagaa gcattagtaa gatggcaaga cccaaataaa ggtcttatac
    19741 caccagacga gtttattcct ttctttgaaa aaaatggttt tattacaaaa ctcgaccact
    19801 atgtttttga aaaagtatgc agtatgataa gaaaatggat agataatgga gaaactccaa
    19861 tacctgtttc agttaatttt tcacgtgcta atttaaataa tatagatttt ttagaaaaat
    19921 acagaatgct tcgtaataaa tacaacattc ctgctaagct cttagaaatt gaattaactg
    19981 aaacacttgt atttgaaaat ctccaaaaat taattaatat aatagaccaa atccatgaag
    20041 aaggttttca atgttcacta gatgactttg gaagtggata ctcttcactc aatatgttaa
    20101 aagaaataaa agttgacaca ttaaaacttg accgtgcttt ttttagctca cctaatgcag
    20161 ataatattgc tgaaaatcat gtaatagaat ctatagttaa tttggcaaaa aaattaaata
    20221 tgaatagtat atcagaaggt gttgaaacaa tcttgcaaat ggattatctt aaaaaaatta
    20281 attgtgatat gatacaagga tatgtttttt caaaacctct acctcaagaa atatttgaga
    20341 aaatgacttt tggaaaaaaa ctagaagaaa ccagtatgga tagttccgtt taaataaaat
    20401 aaaaaacttg tctctataaa ataattttaa tcattttata aagacaagtc tttttatttt
    20461 ccaatttaat tttattttct aaatttctta cactcggctt gttttaaatt ccctttttaa
    20521 gaataataat acaattatca tagcaataaa atctgcaact ggctgtgcca accaaacacc
    20581 atctaaatta aattgaattg gaagtacaat gattaatggt ataaataaaa taaattgtct
    20641 taaaagagtt aaaaacattg aatgtttaac tttacttata cactgaaaat acacaggtcc
    20701 tacaatagaa atccccatta taggcaatgt aattagattt aggcttatac ctcttactgc
    20761 tatatccatc aaatctgaat ctttattaaa tatacctaca aatacttctg ggaaaacttg
    20821 tactactaaa aatccaaatg acaatatgac aactgacact atgatagata atattaaagt
    20881 tttctttgca cgctcatatt gttttgcacc ataattgtat gatattatag tttgcatacc
    20941 ttgattcaat ccaaacacag gcatcataaa catcaataaa atagatgtaa gtgctgtcat
    21001 agctccaata gctaaatctc caccataaac ctttagagaa ttatttgtaa acatgtgagt
    21061 tatacttata gctacttcca tagcaaaagg tgtcattcct attgcaaata ccttctttac
    21121 tatatcttta ttcaatttta agttacattt ttttagtttt aaattggact tccctcttat
    21181 aaaataatgt attacccaaa agaatactac tatttgacag agtactgttg ctatagctgc
    21241 tccttttata cctaaattaa atacaaatat aaatataggg tccaatatca ggtttaatac
    21301 acatcctaca atcattgtcc ttgctgctaa ttttggatta ccttctgccc taattgcatt
    21361 attaagagaa aatgcaacta aattaaatat acttccaacc aatatgacac tcatatagtc
    21421 ttttgcataa aatattgaat ctttacttgc acccaaaata aaaagtatat cttctaagaa
    21481 aactaatcct aatatagtta taattaaaga gataattata gatagtgtca aagcattacc
    21541 taatatcttc tctgcttctt ctctatttct ttcccctaac tttatagaca atcttgtgct
    21601 agcacctact gataccaaca ttccaaaagc tattattaga gtaaatactg gcatagttac
    21661 accaagacct gcaatagcta tagagccaat accttccata gaaccaataa aagctctatc
    21721 tactacatta taaagtgaag ttaccatcat tgctattatt gcaggtatcg aatacctaaa
    21781 taataaaatc catatttttt catgtcttaa tgcttcttga ttctccatgt tgtcaccatt
    21841 tacaccatgt cataagttat acaaattggt ttatttgttc ttgggtctgt tgctatttct
    21901 gcatcaatat taaataattc ttttagatta tccttcgtca taacatcata tgcatcacct
    21961 tcacaaacta ttttaccttt cttaatacct atcatgtggt cagcaaatct tgctgcatta
    22021 ttaagttcat gtattaccat gacaatagtt cttccttgtt ttttatttag ttcatctaat
    22081 aattttagta tctccaattg atgtgccaaa tctaaatatg ttgtaggctc atccagtaat
    22141 aatatatcag tttcttgtgc caaagccata gctatccatg ccctttgtct ttgtcctcct
    22201 gataaatctt ccatatttct atccttaaat tctgcaattc cagtaacttc taaactccaa
    22261 tttacaatct ctctgtcttc tttactagaa ttaccaaatc cacttttatg aggaaatctt
    22321 ccataagaaa ttaattctga aacggtaaga ccactaggag cttgagggct ttgtggaagt
    22381 acagccatat cttttgctat ttctttagac ttctttttgt agatgtcctc tccatttata
    22441 tatattttcc cactattagg gctaataatt ctacctatag ttttaagtat tgtcgatttt
    22501 ccacaaccat tagcccctat gatggtagtt atttttcctt ttggtatatt taaatttaaa
    22561 ttttttataa tatctgtgtt tccatatgat acatttagat tcttagtatt tatacagttc
    22621 ataacaacac cctttcctaa tcatttacca acattaaata aataaagtac ggcactccta
    22681 ttatagaaac tactatacct actggtattt ctataggtgc tagaagattt cttgatatag
    22741 tatctgctat taaaagtaat aatgttccta tcaatgctga tactggtatt aatcttttat
    22801 gctttggtcc tattattttt ctacctatat gtggagctat taaacctaaa aatgaaatac
    22861 tacctgctac tgctgtcgat acaccagata aaattactgc atatattata agtcttcttc
    22921 tttgtttttc tacatctaca ccaagactta tagatacttc atcacccaaa tttaaaacat
    22981 ctatatattt agatttataa atagttatac ccataaaaat cactataaat ggaagcacag
    23041 ctattacata tttccaactg gttccccaaa tgcttcctga tgtccatgcc ataaccctat
    23101 taaattcctg tgttgtaaat ttcaattgga atattgtcaa aattgaagta aaagctatgt
    23161 ttacacctat accaataagt agtaatcttg atgaatttat accacctttc catgcaagag
    23221 aatatataag aaatgctcca aataaagctc ctataagtgc aacaataggc attgtaaata
    23281 cagtcaagtt actcatacct tcatatacat taccattcat aaggtatata taaactacta
    23341 caaacaatgc agcacctgaa tttattccca aaattccaga atcagctaaa tcattctttg
    23401 taactccttg aagtattaca cctgctgttg ataatgcaga accaaccaaa agtgctatta
    23461 ctattcttgg taatcttaat ttaaatattg caatttcatg attacgctga ccctgcccta
    23521 aaaaagtctt tataacatcc attggttcta tagaaaatga gcccatattt aaactcaata
    23581 aaaaagtgat aaaaataagc accattaaaa ttgatataac aagtaatgtt ttagaattta
    23641 tttttataat atttttcata gtacctattt ctcccccttt ctaacaaggt atataaatat
    23701 tggcactcct ataagtgctg ttatagaacc tactggtgtc tcatatggag gatttatcat
    23761 cctacttaat atatctgtat atacaagcaa tacagctcct aatactaaag agctcggtat
    23821 tatatactta taatctgaac ctactattcc tttagctatt tgaggaacta taagacctat
    23881 aaaaataata ttaccagaca ctgacacaga tgctccagtc attaatatta caagtactat
    23941 acatataaat ctaacaaagt tagttttttg tcctaatcct attgccactt cttctccaag
    24001 acttaatata gttattcttg gagccataat aatagctaaa ataaatccaa taccaccagc
    24061 tacaaatagc aattttatac cttcccactt cactcctaca agccctcctg atatccagaa
    24121 acttaattgt tgagataggt taaaatacat agatattgct gatgccaaag aaattagcaa
    24181 tgtaccaaga gcgactcctg caagagctaa ttttacatta ttaactcttc ctcttgattt
    24241 agaacttata aaatatataa ataatccact tatacttgct ccaataaatg caaacatcat
    24301 tacactaaaa cttccatgta taagttgagg aagttttttt tgaagtacca gtgatatcga
    24361 aattgcgaat gtagctcctt gtgtaattcc tataacagat ggttcagcta taggatttct
    24421 cgttattcct tgcattattg ccccagaaac tgctaaaaat cctccaacaa gtgcagctgc
    24481 tattgccctt ggaagtctaa catcctttac tattctcata tttattccat cttccatttt
    24541 tatcaaacta tctataacag ttgatagatt catatctttt gccccaatag ttatggataa
    24601 tacaattcca aatacaagta caactatact tactaatatc aacaaataag ttatattttt
    24661 tttactcagc ttagtcaatt atcctacctc cttttccctt ttaatcgtca gtataatatc
    24721 atctattaag tattttaatt ttaagattta ttaaaaatag gtgtgaaaaa tgaatatcat
    24781 tatctcacac ctactaagtt aagaattact ttgttaattc atttttaact gattctaata
    24841 ataactcttt tccaattgga ttgtatgatt gactgaaaaa tggagcagca tcaagtattg
    24901 ttacattacc atcttttact gctcttattt gagcccagat agcactattt tctaaatctt
    24961 ttttatctgc ttctgtagca attacaacta tatggtctgc atctatatct gttaaacctt
    25021 ccatacttac cattggtagt gttattccat cctgttttgg catattctta ggtttagcta
    25081 atttcatatc atcattgatt agagtaccta ttccaccatc actaaatacc ataaattgac
    25141 ctgaacttgc caacactggt aaataagttt tttctccatt cttttctata acttccttac
    25201 ctaactttgt agctttttca tcatacttct gtagccaact tttagcttct tcttctttgt
    25261 caaataactt tgaaacatca gttaacttgc ttctccagtc atttgcataa tctttcatca
    25321 taactacagg agctatttct tttaattggt catatatttt ttcttgtctt tgactcatta
    25381 ttattaaatc aggatttact tctaatatag cttccatatc catagtatcc atcattgagt
    25441 gcccaactat ctttacacct ttaagctctt ctcttatgta acttggtaat ttatcagttt
    25501 catatgagtc tacatttgct gttgcaacag gcttataacc tgctaataat agttcttcac
    25561 tacttcctga tatatcaact atttttttag gatttgatgg tatcttaacc tctcctttaa
    25621 cagattgtac aactcttgtc tctgatttat ctttatcctc tgtattctta tctgaacatg
    25681 ctgtaacaag tactaacgta gttataatag atataaatat tgcaagtgat tttatttttt
    25741 tcatgattac ttctcctact tgtttattat acttacatga tattgattat cataagcaca
    25801 tatttattat agcatttttc tatttttagt caatattatt ttgtaaaagg ataaaaattc
    25861 ctaattaatc ttaattatat atagcttaga taaggttttc ttatattaaa cagtattttt
    25921 ttaaagaatt ggacacaaat acaatgactt ttcagttaaa caatgataaa atgcaattta
    25981 tagataatga atttagtaag gaggattcaa aacatggtac atttagtata ttgtgataat
    26041 gcaggaaaaa aaggagaaaa ggtcttagat aaaatcttgg ctggaacaaa aacaatggtt
    26101 attagaggtg ctgctggtag aaaaatacca catagtagag tatttgaaga tgagttactt
    26161 tattttatgg aaaaaggaac tgcaaaaata tctgcaaaag ccactgttaa aaaggtagaa
    26221 aactatgtta aattaacaga agatgaaatt tctaaaattt taaatgaaaa tcaaccaaaa
    26281 ttaaatttat ctgaaaagca aaaagttcgt tggcataaaa aatgtttatg cttagttgaa
    26341 tttgaaaatg ttgaagaaat aacacctctt gattttgacc atcaaggaaa tatggatgat
    26401 tggcttattg ttgaaaaaat tgaagatgta gttgttggta ctagtatacc ttacaattat
    26461 gagaagtcta aattttaaag tatcaataac ataatactat attgctaaac tctacttaaa
    26521 taaaaaaaga gtgcatttaa taattataaa aattattttt atacactctt ttttaataat
    26581 aattttaaat tacttacttt ctaatctttc atatacatct ataaattctt ctgctaattg
    26641 ttgaaaattc atagctgtca ttaaatggtc ttgtgcatga ataagtataa cacttatttc
    26701 agttttatta ccattagctt cattttgtat caattctgtt tggaatttat gagctttatg
    26761 aattgattct ttcgactttt caatacattc tctagcttta tctatattcc cgctctttgc
    26821 ttcttttatt gcttcaaatg ctagcccttt cgcatcacct gcaaatccaa ttattccaaa
    26881 agatatctct atcatcttct catccattta ttcgacctcc taaatatctc tatagcttga
    26941 tatgactaat ccattctttt ctaaaaattc tttgactttt ttagatgtaa gtatcttgtg
    27001 ctctttcgtt ctatcaatag cataagatgt agaatttaaa atataatcat ctaaaaaagc
    27061 tgggtgagac ataatatcca ctacatcata cttcattatt tcgtctatat tttttataaa
    27121 atattcttca cttacatttt ccttataaaa actatctatt aaaggaacta ctttagaata
    27181 ctctaaatta tattcaaatc cacctcttat tggaagatta tacttggtta caatcttctc
    27241 tattactggc tttaaactta ataaagtatg gatatgatgg tgactatcta aatgagatat
    27301 atctattcca agccctttta ccctatctat ttgagcacaa agttctctat aaatctcatc
    27361 acaatccatt ttttcaataa tttcgtctgt aatcctccta ataaaaaaac cgtctttatc
    27421 aacaatagtt tttaagttag ataataatgg tctaccacaa cttaaagtca tatgaactcc
    27481 acaatttaaa gttttatttt ctttaagtaa gcctactcca tgttctaccc caggcatatt
    27541 tgccatcata gaagtagacc taactatacc attattatga gctgaaatta ttccatagtt
    27601 cacagcttca caatatccaa aatcatctgc atttattata attttagtca taccataacc
    27661 tactttctat agacaactat ctattaaatc taggaagata atctttatga gcttccaaca
    27721 tttcgtctaa tatagttttt gctaagtcat cagatggaat caatgggttt atacaaagtg
    27781 ccaaaagagc agattcataa tcgccatata cagctgcttt agctgccaat acttcaaatg
    27841 atttgatttg acttactaat ccatgcacag aatctggtaa atatcctata cttattggtt
    27901 ttggaccatt ttttgttatt acactactta cttctaccgc ttgttcatct ttaaaatctc
    27961 ttatagaacc cttatttaat gtattaacta cttggatatc ttttttgtcg ttgtatatag
    28021 aacttattaa attacaagca gcatcactat agtaagcccc tcctcttttt tcaagttgag
    28081 gtggttttac atctaatttt tcatccttat ataattcaaa taattgttct tccaattctt
    28141 taactacttg acctctagct tttccctctt taaactctct caactcatcc tcaagcattt
    28201 ctttagtttt atagtagtat ctatgatatg gacaaggtat tgcacccaaa gattttacaa
    28261 attcagcatt aaaatcaatt gcctttatat tttgcatact tatatctgct ttgacaaatt
    28321 tatcaattgc ttcttttgta acatcttctc catctaaaga tacatttaga ccataaacca
    28381 tatgattaag acctgcaaaa tccatactta ttctgtcact ttctacatta aatagtttgg
    28441 caacatcatt ttttaaatga attggcacgt tgcatagacc tatatatctt ctaaaattag
    28501 tatatttgaa tacagcctct gttattattc ctgttggatt tgtaaaattc accatccatg
    28561 catttggaca taattcttcc acatctttta ttatatcaaa tataactggt attgtgcgaa
    28621 gggctttaaa taaacctcca gcaccattag tttcctgccc cataactcca tgagataaag
    28681 gaatagtttc atcttttatt ctcgcatcta aaagacctac tctaagttgt gttgtcacaa
    28741 agtctgcatc tcttagagct tctcttctat ctaaagtaag atttattttc atatctactc
    28801 cagctttttt taccatcctc ttagctagat ttccaactat ctctaatttt tcttttccag
    28861 cttctatatc tacaagccat aattccttta caggtaactc ttcatatcta tttataaaac
    28921 cctctacaag ctcaggagta taacttgaac ctcctccaat tgtaactatt tttagtccct
    28981 ttttcatctc attataccat ccttacctat aatttaatat actctacaaa accccataca
    29041 tttatttaaa actctataca tttatttacc gaaataaaac tacctagttg ttaattttca
    29101 atttattagc ttccattttt tcagacgcta tgacaaatgg aatatatatt aatattgata
    29161 atattaaatt aaatagtgct aaagctccac cagtccagtc acctgtagcc attgctccac
    29221 ctaatatagg aggtgttacc caaggtattt taggtaaaat tactggtgct actaatccaa
    29281 aatctattgc taagtatgat acagcactac atattactgg tgcaagtaca aatggaatca
    29341 taaatatagg atttaaaact attggaagtc caaatatcaa tggttctcct atgttaaata
    29401 ttgcaggtgg accacctaat actatcatct ctttacgtct tcttccagct attaataaag
    29461 ctactattaa tcccaatacc attccagaac cacctaaata tacaaatgca tcaaagaatg
    29521 cacctgcaag tacatggtat ccttccgttg ctcctgcttg agccaaggca gcattttctc
    29581 cacctaattt cataagtata gtttctgtaa aaggaagtgc tatatttgca ccatgaagtc
    29641 caacagtcca aagtatatga attataaatg caataagcat tgtagaacct agactatcag
    29701 ctacatttga tagtggtgct cctaaataag tatttaaaat atctgttaag aaacttccat
    29761 tagaaagcat ttttataaat attcctatta cagtaaatat cattatagtt aacattcctg
    29821 gtattaactt tgcaaatgat cttgaaactg ctggtggaac tccatctggc atttttatag
    29881 ttaatttctt taccttagat aatcttacaa atatctcagt tgataataat cctattaata
    29941 tagaagagaa taaagctgct gtacctaagt aggtttgttg tatcattccc catccttcaa
    30001 ctactgtagt tcctccctca ggaactattt taccaatttg tggagccatt ataaaaaaga
    30061 tagataaagc tataagccca gattgtaacc catcactctc ataagatttt gctagaaaat
    30121 aagcaacacc tattactaag aatactgcca ttgtacctat tgctccccac caaaggtcgc
    30181 ctccaaatgt ggtccagttt tctccaaata tacttgccat caaatttttg tatgcttcca
    30241 atggtaagtt atttatcaat gttcccaatg cacctaccat agttataggt atcattacta
    30301 taaatgcatc tcttactgct actaagtgcc tttgagctcc tattttagca gctacaggca
    30361 ctatatattt atccataaat gacatgaatt tttccatttt acatcctcca gtacaacttt
    30421 attttgaact atttaactct acagctctat ctaacacagc ttttccatta catcttccat
    30481 aatccatcat atttataact tctactggaa tattatatgg ttttgccatt tcagttgctt
    30541 ttcctaatac atatctaact tgaggcccta aaagtaagac atcacaattt tctatttcat
    30601 tttttaagtt cacttctgat atagcccata tttttgcttc tataccgttt tcctttgctg
    30661 catcctccat ttttgtaact aataaactag ttgacattcc tgctgaacaa gctaacatta
    30721 tttttatcat tttttcccct ccaaattaga ttcattaaat attttttaag tcataagttt
    30781 tttaaactat acatttaaaa tatgtttttc tattaatttt ttcttaatgt atatatctta
    30841 tttttaaatg tatatacatt taattcttga tattattata taccaaatgt atatacattt
    30901 caagaaaact ttttcatttt atttaaattt tattcgacaa cagcattcaa agttaatgta
    30961 tatccatagt gatatgtgtg tgagtactca aaaatctcac tattttgtaa cttacaaatt
    31021 tggcttatat ataaaagtgg ctctggttct tctaatttta gattatccat acactcttca
    31081 ttcgatatta cagcttgtac ttctctctct gacatggcta tcttgtatcc acacacttct
    31141 tcaacaaaat tataaagaga attttcacaa tgagtcttat ttaaatttgg aaataactta
    31201 actggcatat aagtaatcat atatgaaact cttacatcat tagctaatct tactcttttt
    31261 atttcccaaa cattttcttc taaacttata ttcaacttac ttgataaatt ttcatctgcc
    31321 tgaactattt caagagatac caatatatta gatactttat agcctttact ttccatctct
    31381 ttagtaaatc cacttacaga cgatatatta tcatatatat tatttgcaat aactatacta
    31441 cctacacctc tttgtctagt tatatagcct tctttaacta gattattaag tgcagacctt
    31501 acagtcattc tagttacatt aaattcttca ctcagctgtt tttcagaagg gattaaatcc
    31561 ccttctttca aactgtctga atttattaac tctctaacat gattttctat aactttataa
    31621 attggctctt tcatttaatt cactccttat taaatctttt atataataag ataattttat
    31681 atacaaacaa agaaaatgtc tatacattta agataattta aattttattc agaaatcttg
    31741 tacaactttc cgagttttaa taaagatata aatagactta tcattaatat accttttaat
    31801 atacttgata aggttatact ccaccataca ccatttagac ccaacatttc tggtctagat
    31861 ataaagtaag ccaatggtac tcttgcacca gtcagtatag taattataat agaaggtata
    31921 taggttctcc ctaatccttt gaaagctcct gttgttatta tttctaaaca cataaaaaat
    31981 tgagaatagc ctaatatttt aagataaact gctccttttt ctattgcctc actttcatct
    32041 ataaaaatag aaaatatact ctctcctgca aacactaata ctaaagtagt tattatacca
    32101 agtacagaag atacagttat agctattttg caacctttat ttattctaga atacttcttg
    32161 gcaccataat tttgtccaac aaaactgcct aatgcaactg atagcccttc agccgtcatc
    32221 caggaaattg cttctatttg tgagcctact ttttgtactg caacagccac aggcccccat
    32281 gatgctacta ttactcctaa tagcattgag aatactgtaa acataccact ttgtattgct
    32341 ataggtaatc ccaatttata cagaacttta taatatttag tctctatttc tttaaatagt
    32401 cttaatttaa aatattcttc tttactttta actattatat atataaaaca tgtagtaact
    32461 actatctgag caaatacagt tgcaatagca gctcctgcaa caccaagttt tggagcaaat
    32521 ccccatccaa aaattaatat agggtcaagt ataatattag ttattaatcc tactgtatta
    32581 atacgaaaag gagttttact atttccaaga ccattgaaaa ttgcagtaaa tactggatta
    32641 ataaaataaa ataccatacc tagagctact attatcaaat actgtctaga catggttata
    32701 acttctaagt ctcctaatct aaaaaaacct atcaattgtt tattaagtac tattaaagaa
    32761 attgtatata ttattgctag tataatattt atttctattg ctgacttcat atatgattta
    32821 gtagtgctta tattatttgc accaatactt tgtgctactt ttatctctcc acctactttt
    32881 gaaatcataa taaatgccat tgcaagccat ggaaaaaaac cagctgttcc aactgcagct
    32941 actgctttac ttccggcttt accaacccat atcatatcta tcatgttata ccccatctgt
    33001 ataaatgaag tccccattat tggaagtgat agcttaagta atttttctgt aattcttcct
    33061 tctgttaaat taagtctgtc tttcattttc cacctctttt atattaaatt tatttgctta
    33121 tatagctaga ttaatcattt tatttgattg agttcactat ccttcaattt atattatcat
    33181 atctcaggat tgcatctcaa tcatacttat gtttaagata cagtatttta cctatctatg
    33241 ataaaattaa tacttatgaa tataaatcta gccatttcat caatacatat tctaaccttt
    33301 aacttaactt caattaagta tacatatcgt aaccaattag aatattttat acggtatctt
    33361 ccattaataa acaaccaata tcaatgattt atatataatt ttatttatac ataaaatagt
    33421 atactataat aataaaaaaa ctatatttga atgctttaag catgtttact aattgtaatt
    33481 tccacacaaa tttgataata tatgaatata ttatggtgaa atttatagat atatattaat
    33541 tatggggaga ttgtgggagg ttcttttcac aagacattta agctatgaaa ataaataaaa
    33601 aattcaatag agttgatatg caggtttcaa tacttactgc aattattgta ataatatctt
    33661 cctcatgtgt atattttttt aattatacac taacttataa gagcatgata tactctttaa
    33721 gtgagaggtc aaaacatatc tatgaatatg tagaaagaaa tattgataaa aataccttta
    33781 ctgaaattaa ttctaaagaa gaccaatcaa aaaagtctta taaaagttct aaaatccttt
    33841 tagaatctgt aaaaaatact actggagtca tgtatctata tactgctaaa aaaacaaaag
    33901 atggatcact agtatatgtt gttgatggtt taaattcatc taggagtgat tttagaaatg
    33961 ctggtgattt gatagaaaaa gagatttggg gagaccttaa caaagcatta gataatcata
    34021 tattgttacc taaaaagatt aaatcaacat cttggggata catatttata tcgtattttc
    34081 ctattcacaa tgatgttggt aaggttgttg gagtaatagg aattgaattt gaagcaaaac
    34141 atcaatacaa tacatttaaa tttataagta tagttacacc aataatagca ttattaactt
    34201 gtctaatatc agcattgatt gcagtgattc tatttaaaag aatatctaat cctacatata
    34261 ctgattttgc taatacagat tatttaacag gcctaaaaaa tagaaatgct tttgaaatag
    34321 atatgaataa cttaaacaat tcaaatctaa agaatctaat atctatcata tcaatagatt
    34381 tagacggttt aaaaaagatt aatgataaat ttggccatca gacaggagac ttatatatta
    34441 agcaagcaag taaaattata caatatgtaa taaataaaaa atatatagtt tatcgtattg
    34501 gaggtgatga atacataatt attctaaaaa atttatctca caaagaaata aataatatta
    34561 taaataatat taatttaaaa atagttgaag aaaactcaac taatgaatta aaattatcaa
    34621 tgtctattgg atatgctatt tttgatgaaa aagtagatgt ttctttattt gatacatatc
    34681 gtcgtgcaga ttctcaaatg tatgataaca aaaggaaaat taataaacaa tatcaataat
    34741 aaatttaaaa agtctattct ttaaataata aatcacaaga atagactttt ttaaatttgt
    34801 gtttttataa gataaattat ttaattaaag tttgttcttt tttctaaacg attctttata
    34861 aattcatata ttactttcac taatatattt gtgactaata ttaacacagc tagtgatgct
    34921 gcctgtgcag tactcccctt atcatctagt tgtacaattg atattgcagc tacttttgaa
    34981 cttgcagtat atataaaaac taatgctgat attgtcacca tggagtttag gaaaaagtac
    35041 ataaagttct ctaaaactgc tggcaaacaa agtggtatag ttacgttctt aagaattgat
    35101 gcgctgttta ttcccattga tttagctacc atatccaatt ctttatctac tttcttaata
    35161 gcagtagtag ctgttataaa tgctactgaa aagaaatgaa ctatattaca tataaccatt
    35221 ataagtaatg ttccatataa tccattaaac aaattcttaa tgtatattgt atccataaaa
    35281 ttaaactcta atttattaaa aaataataca taagctatac ctaaaactaa tcctggcaat
    35341 gccataggca atgtacttaa aaaataccca aatgatttta aatatggagc tttttctgtt
    35401 ttttcattca aatatgcaaa taagaaaaca aagctcgtcc ctagaatagc tgtcaatagt
    35461 gacattttta ttgaatttat aaatggttta atcccatcaa tactgttttt catagaaaaa
    35521 tgttccagtg tcagtcttaa atcatatggc caaactgata ttaatgaaga taagattaca
    35581 gttaacatta cagctattat accaaatgca ataattccta caacccctcc taaaatacta
    35641 tctcttaact tattagtttt aaccttatat ggaattgact ttgaagttat atatgaattt
    35701 tgattttttt caattttttt agttactata aacgatatta ttgcaggtat taaaagtaat
    35761 attgatacag ttgctcccat tggcatattc tgttgcccta taatttgctt atatacatct
    35821 attgataata cattaaactc tcctcctatc acctttggag ctccaaaatc tgtaaaacat
    35881 aatgtaaacg atacgaatgc agaacttatt aaagagtatt ttatacttgg tattgttatg
    35941 cttataaatt ctcttatctt gcttatcccc atagtattac tagcttcata tagtctataa
    36001 tcggttgaac ttagagatat ataaagtatt tgaaatgatt gaggaaatac atatataatt
    36061 tgtgccaata ttattcccac tgctccatat attggaacat gcactccaag taaattagtt
    36121 accaaacctt gatttccaaa taaatatact agacctattg catgcatcat tgttggagca
    36181 aacataggaa acatagctaa tgatttaaaa aaacctttaa atttaatctt tgttctagtt
    36241 aatgcatatg catatactaa tgcaagtgtt agtgatatta cagttgatat aactgataca
    36301 aatactgtat tttttagaga atttaataga gattcagaag ccaagtattc ttgaaagttt
    36361 tgtaatccta caaagactcc atctttatct aaaaaagcct gtacaaatag atatattata
    36421 ggtgctaata aaaatattac taaagacata actaatactg taagcataca tttttttata
    36481 tctaaattca tttttgattt aagacttata cttttagaac ttatagcatt attattcata
    36541 cacttatgct cctttaactt ttggatactt taaatagtat ttttcgtcta tacttatttt
    36601 tacaaggtca ccttctacta aatttaaatt aacccactct tttatagaag catcacttat
    36661 tatgaaattt tcatttaatt tatcttttat ttcaactctc aataaattcc ctctaaactc
    36721 tatatttgat attattcctt ggtagttttc ttttgtcgat ttctctattt gaacatattc
    36781 tggtcttaca gttaatatag aatctccatt atcaaaacag tttgtatctc ctataaattg
    36841 agctgtaaat aggttttgag gattttggta tatttcttct ggagtcccta tttgcattat
    36901 atctcctcca tttagaatag ctattttatc tgccatagtt atagcttctt cttggtcatg
    36961 tgttaccatt attgtcgtta tattaagttc tttttgtaat cttttaatgt ccattctaag
    37021 cttgtgtcta acctttgcat ctaatgcgga cattggttca tctaaaagta aaaactttgg
    37081 ttcaagagct aatgctcttg ctattgcaat cctctgttgt tgcccacctg ataatgcttt
    37141 aggatattta tgagcttcat tagtcaatcc aactgtttca agtacttcct taactttatt
    37201 atctattttt ccttttgaaa cttttctctc ttttagtgga aaagctatat tgttataagc
    37261 agtcatattc ggaaataatg catatgattg aaatactata ccaaaacctc tctttgatgg
    37321 ctctaaattt gtaatatctt tatcctggag aattatagtt ccgctattta catcttcaag
    37381 acctgctatt attctaagta atgtagtttt accacatcca ctaggtccga gtaaacatag
    37441 aaattctcct tcttcaatat ccaaactaat attatttaaa accctttttt ggtcataact
    37501 tttaaacaca ttatttattt ttaagtaact catatcttat ctctccttat atagttattt
    37561 ttttatttta aattttatat cctacttttt cttctaaaat tctttcatct aaattattaa
    37621 tttaaacttt acttactttc agattttgaa ccgtattttg attcccaagt tttaagtact
    37681 gtacttctgt ttttggcagc agagtttaaa tcaacttctt ttataagctg ttcaaatggt
    37741 tgtttagaat aaccttttgg aactggatta tctgtcttta tggaagtaac tgctacttca
    37801 tgtgcatatc tatccatagc tgaatcactt attgcccaat ctaaaaatag ctttgcttct
    37861 ttttttatat tatctttttt aactaaggca tttgcttcta agtcccagcc tgaaccttcc
    37921 tttggaaata caacctctat aggttctcca ctagataatt gttgtgtagc tctatatcca
    37981 agtgtaatac ctattggata ctctccagag ccagctaact tcgcaggttt tgaacccgag
    38041 tgtgtatatg tggctatatt tttatgtaat ttatccatgt aattgtaagc atctttttca
    38101 cccatcattt gaataagccc tgatactgta agataccccg ttccagatga tgctggattt
    38161 ggcattgtta taagtccttt atattctggt tttattaaat cttcataact ttttggaata
    38221 tctataccta gtttcttaag ttccttataa ttaactacta ttgctgtctc tgggacactc
    38281 attcctaccc aagatacatc atcttcagta tctttaaatt ttttatcaac tttatcataa
    38341 tttttaggct tatatgcttc aagtgcacca tcttttttta gctctatcat gttcattgct
    38401 gataatcccc atataatatc tgcttgtgga ttatcttttt ctgctaatag ttttgaagct
    38461 attacaccat gagaatccat aactatctct acatctatat tgggatactc ttctctaaaa
    38521 ccttccatat aataagctat ctgctcttcc tctagtgctg catatattgt aagtttatta
    38581 tttccttttc cttgagtagt ttgtggtgtt gttgatatgc aacttgtcat aaatgtaatc
    38641 actacaatgc acagacaagt aaatataaat tctttcatat cttccccctt tgtgtttttt
    38701 ccttttttta tttgtttttt attgtttttg cttatgttta tataatacac ctaatttgtt
    38761 aatataaagt taagtgtaaa ttaacagttc gttaattttt agaaattttt gttaaatata
    38821 ttatttttag ttaaatacag tctgttttta aacaatatat taatttttat agtatctaca
    38881 ttttaattta aacataaaaa ataagtctaa cctctataaa gtagaatgtt agacttattt
    38941 ttataagtat ttaattatac aaccaaatca ttttttttct tttcttcaag tgctttttta
    39001 agcaccatat ccttaacctt cataagtcta gtagtacttg ctctctcatt ttcttcaagc
    39061 ttcatagcta tatacttaat agtctcaatg taatttggta tcattacatt ttcaagagca
    39121 ttaacacgtc tacgtgtctt ttctatctct tgtgcaagaa gctgtgcact tttttcactt
    39181 tctgcaagtc ttaaaagtgg ttgcatagca tctgagaaag cttccatagc agaatctaat
    39241 tcacctgatg taaaagcaag accataagga taaatatcac tttgattgtt ttcagtttta
    39301 aagtcaaaca caggaacatc aacactcatt atatttctcg tagaaacatc tactgaaact
    39361 gattgctttg gcatcataag tgctgaaccc agatactcct gactcataac tgctcttgcg
    39421 attataaagt ttttataagc agtatcaaga gcattttctg cttcttcacg caatcgctta
    39481 ttttctctta caatctcaag aaattgtttc ataagttcat caagtttatc tttaagaagc
    39541 ttatgacctc ttgtagcagt tttaaggagt ttcttaagtc tagtcatttc catacgtgtt
    39601 ggatttatat ttaatctagc cacagatatc actccttagg catatatttt tcaaaatatt
    39661 catcacggat acgtttaagt tcactttttg gtaacatact aagaagcttc catccgattt
    39721 ctaatgtctg ctcaatagtt ctatcagttc taaatccttg tgaaacatat tctttctcaa
    39781 attcatctgc aaacttagca tacattaaat caatctcaga aagtgcagat tcaccaagta
    39841 tagccataag ttcttttgca tccttacctc ttgaataagc tgcaaaaagc tggttcattg
    39901 tatctgcatg gtcttcacga gtcttacctt taccaatacc tttatctttt agacgagaaa
    39961 gtgatggtaa aacatctata ggaggctgta tatctttttt ataaagttca cggctaagta
    40021 taatttgccc ttctgtaatg tatcctgtaa ggtcaggaat tggatgtgtc ttatcatcct
    40081 caggcattgt aagtattgga atcatagtta tagacccttc atgccctttc atttttcctg
    40141 ctctttcata catagtagca aggtcagtat aaagatatcc tggatatcca cgacgtcctg
    40201 gaacttcttt tttagctgct gaaacttcac gcaacgcctc tgcatagttt gtaatatccg
    40261 ttattataac aagtacatgc atatcttgct cgaaagcaag gtattctgct gctgtaagtg
    40321 ccattctagg tgtagataca cgctctacag ctgggtcatt tgcaaggttt acaaacacta
    40381 cagaacggtc gattgcacca gttgctttaa aatcgcttat aaaaaattca gcatcttcaa
    40441 aagttatacc aactgcagca aatacaacgg cgaacttaga atcagttcca cgtacttttg
    40501 cctgtcttgc aatctgaact gcaagattgg catgtggcaa accagaccct gagaatatag
    40561 gaagtttttg ccctctaacc aatgtattaa gtccatctat agcagagact ccagtttgta
    40621 taaactcggc cggataatca cgtgctgctg gatttattgg agctccattt atatcaagtc
    40681 ttttatctgg tataatttct ggacctccat caataggtct acccattcca ctaaaaacac
    40741 gacctaatat atctggtgaa actccaaagt caagactctt tcctaaaaaa cgaactttac
    40801 tttcagcaag gtttatacct gttgagcttt cgaaaagctg aacaagtgca gtatctccat
    40861 ttacctctag aactttgcaa cggcgaattt ctccatttga aagttcaatt tctccaagtt
    40921 catcaaattt tacaccttct acgccgttta ttaacataag aggaccggct acctcttgta
    40981 ttgacttata ttcctttatc atagttcttc agcccccctc tttttaataa ggtctgcaag
    41041 ctcgttatct atttcatctt caattgaatc gtaagctgca tctatatcgc cttcttctac
    41101 atacttagca cgaccaatct tttcaagaac tgaaagcttg ataatatcgt ttatagtaac
    41161 attttgcttg atagcatctt gtgctgactt atagaatttt aatataagtc ccatcattct
    41221 atattgttta tgaagtgatg tataagtatc aacttcatgg aaagagtttt gatgaaggaa
    41281 gtcttctctt atagaacgag taacctctag tatcaatcta tctccatctg aaagtgaatc
    41341 aaccccaacc aattgaacta tttcttgaag ttcagattca acttgtaaaa gtttcatagc
    41401 ttgagctctt tgagctgaga aattatcatt tacattttta tctgcccata catcaacttt
    41461 ttcctgatag agtgaataac tagtcaacca gttaattgct gggaaatgac gtttatatgc
    41521 taaagatgca tcaagacccc agaatacttt tactatacga agagttgctt ggctgacagg
    41581 ttctgatgta tcaccaccag gaggagaaac tgcaccaatt gctgtaagtg ttccttctct
    41641 ttcatccata cctagacaat ttacctttcc tgctctctca tagaattgag caagacgtga
    41701 acctaagtat gcaggataac cttcttcacc aggcatctcc tctaaacgac cactcatctc
    41761 tctaagagcc tcagcccatc ttgatgtaga gtctgccata agtgcaacac tatatcccat
    41821 atctctaaaa tattcagcta ttgtaatacc agtgtatata gaagcttcac gtgcagcaac
    41881 cggcatatct gatgtatttg ctataagcac agttctttgc ataagtgact cgcctgttct
    41941 tgggtctttt aattcaggaa attcaagaag aacatctgtc atttcatttc cacgctcgcc
    42001 acagcctata tatacaacta tatctgcatc tgcccattta gcaagctgat gctgtgtaac
    42061 agtttttcca cttccaaaag gtcccggtat agctgctacc ccaccttttg tgattgggaa
    42121 aaatgtatct ataactcttt gacctgtaac aagaagtgaa tctggtgttt tcttttctct
    42181 atatggtctt tcttttctaa ctggccattt ttgcatcata gttacaggta tatcattacc
    42241 tttttcatct gtaataacac aaactgtatc ttcaacagta aattcaccag aatatatttc
    42301 ttttatagta cccttaactc cataaggaac cataatttta cattctacta ctgctgtttc
    42361 ttgaacgcca ccaattatat ctcctgctac aaccttatca ccaacagatt ttttaggttt
    42421 aaattcccac ttagttgttc tatcaagtga agatacttca acacctttag ttatattagt
    42481 acctgaaata tcatacattt tttcaagtgg acgttgaata ccatcataaa tagttgaaat
    42541 aagacctggt ccaagttcaa cactcatagg cattccaaga gaaactactt cttcccctgg
    42601 tcctagaccg gaagtttcct cataaacctg tatagaagct ttatctccat gcatttctat
    42661 aatctcgcct ataagatgtt tatctgaaac tcttacgacg tcaaacatat tcgcatctct
    42721 catgccctcc gctacaacaa gcggtcctga cacctttaca attgtgcctg ttttcatatt
    42781 ctctcacctg caatcatata ctttatcaaa atttaaatat tccctcctat attttattag
    42841 cacatatatt tagcaccaat aaaatttgag attgtctttt attcaacttt catctattct
    42901 ttaaataata tatctgcacc tatagcacgt ttactagatt ctcttacttc attcatacct
    42961 aatcccatac ttcctccaat acctggaata actataatgg caggaagttg gtaatctttg
    43021 tattttgcta ttgattcttt tgcaagcaat gatgcctgtt ctgtaatata aattatcgca
    43081 tattcatctt caaccaactt atgtatactt tttttaattt cgtcactatc ataggctggg
    43141 aagatatcta ttccaagagc taaaaatccc atgatgctat ctttatctcc aactactcct
    43201 accttataca taagcatccc ttagcctttc ttttatagta tctgcgtcta tattattaag
    43261 cttactagtc aaaataattc taattgtttt tatctctgat tctttagcat ataaatatgc
    43321 aattagtggt tctaatgtaa gaggcttaaa ttttgctcct ctaacatatt ccataagata
    43381 gtcatcacaa agtttttcaa agacagtaaa accactatcc atgccatttt tacagacatc
    43441 agagtaagaa gtagatttaa aacatgatgc tggagtatct gaggaaaaag cttctaagaa
    43501 tttttcctta tcaagtgagc cacctgaaac aaacacattc ataaacatat caaaatcttt
    43561 cttcatattt ttgactctta taaaactctt tagatttgtt aaatcacaaa ctttcataac
    43621 ataatctatt acaaatttgt tttggctttc tttagcagtt tctttcatac tggtaaaggt
    43681 tgctttatct agaacaatat caaccatctg gccattttgt gtcttattat aaacatcaaa
    43741 tgcctctaat atagctgatg acattatctt aggtaaatca ctaaaactac gatttgctaa
    43801 agattctcta agcttaataa gaggaattgt acctccatca attaaatatt tctcaccatc
    43861 tacaccagaa atttcttctt taatcagcac ttttaaattg tgatagtcat ttttataaag
    43921 aaatacatca acaaattttt cttcaggagc tagactttta agaatttcat atgtctttga
    43981 taattcttga gtcaaaatat tctcaaaatt atgtatatta ttatttgatg tatcagcata
    44041 acctgcctca gctataattc ttaaagagtc ttcagggctt ttggcttctg ccatttgaat
    44101 aaacatttgt ctgttaagaa gctgtttttc aagcactctt attcgtgcaa cggcataggg
    44161 ataagttttt tcttctgcca ttttttaacc cctccttaat taaacaaaat ttctgctgca
    44221 atttgttcaa tatattcatg ctctactgca attatggctt caaatgaata attgtactca
    44281 atgtcacctt ttttaactat gaaacctcca gtaatatctc tagtttcatc agacacttta
    44341 atgccttttt ttgatagcac atcttttaat gtttttttat cttttttaga taatactatc
    44401 tctgaatcat ctgtacaatt tgatttttca atcatgttta gtataatctc ttcatattca
    44461 ttatctttaa gatttaaaag tttgttttta gcttctaaaa ttacctcttc taaaatctct
    44521 tgtttttttg ataatatttg ctttttagcc tccatataag ctcctgatat ttccttagaa
    44581 gccgcttttt ctgcttctgc ctctgcaagc tttgtatagg aagccatttc cttttcagct
    44641 ttttcattag ctgaatttac ttttaaatct gcttcacttt tagctttatc caatatttct
    44701 tgtgcttctt gtttggcatc agctataatt ctgtcaatca ttttttgttc attacccata
    44761 ggagtgcttc ctccttttca ttttttttac ttatgaatta taatttataa ttaaccaatt
    44821 gcaattccat tgtaagctaa gaaagaaaca agtagtccta aaagagcata tgtttcaact
    44881 attgctgcaa atgttatagc tttaccaagt tcttcaggtc tttttgcaac tattccaact
    44941 cctgctgctg ctgctttacc ttgtgctata cctgatacta aaccaactaa accaattgga
    45001 agtcctgcca taagaagttg taaaccttga gctgatgttg gtattgcatc tccaccaata
    45061 attcctgctt ttgataaaat aaggaaagct atgataaatc catacagacc ttgtgtacca
    45121 ggaagaagtt gtaaaactag aagttgaccg aatttactag ggtcttctgt tacaacacct
    45181 gctgcggctt gacctgctat tgaagtacct ttagcagaac ccatacctgc aaataatgct
    45241 gctatagcag ctcctgatat tgccaaaaat tgaccatttc ctaacgcgtt taaaaattcc
    45301 atatttacat ctccttttta ttaatgcttg tatattttgt tttatactta aacggaacaa
    45361 aaggaactcc tccgccttca taaaacttat tgaaaaattc tacatactgt aacctacttg
    45421 tatgaacata tgcacctagt gcatttataa gaaggtttat tgtgtgtcct acaacaccaa
    45481 ttacaactgc aagtatcgga ccagctatag ctcctaaaag gttgataact tgtgctataa
    45541 ctcctgttgt taaacaaagc gccataattc ttgtataaga taatatatca gcaaaataac
    45601 ttgttatacc atagagactt gaaaatcctt taaaaagctt aacaggaata cctttaaagc
    45661 ttcttccttg agtaagtaca agacctatag cacctataat tgctaaatat tttcctactt
    45721 ctgaaaatac acctatatcc cctgcaacaa aaggtattat aagtagacat acacctgtta
    45781 tacacaaata ccaaaaacct atgtcaaaga ttgcatctat tacctttcca cttcttatta
    45841 atgtatatgc ttttattccc aaacctacat atatatggat aataccgaac agtaacgaca
    45901 ttcccataag tgtcataaca tcctctaaag gattaattaa tgcttttata ggaattaaat
    45961 cccccaaaat acccccgaat ataagacccc atacagtagt tgaaactcca cagataccaa
    46021 taagtttaat taaatttcct tcaccttttt gtatctttaa tttatataca acaaatccac
    46081 agactactgc aattattaac ccatatgcgg cgtcacttag catcattcca aagaatacaa
    46141 caaagaagaa agttaaaatt gtatttgggt caatatcttt tgtacttgga tagctgtaca
    46201 tgttagttac actttcaaaa ggtgttacta atttattgtt ttcaagcaga attgggaagc
    46261 cttcttcttt acttccttct tcagtctgaa cacaacaatt aaacttttct gtaatttctt
    46321 taattaattc atctgccctt ttgcttggta accaaccatt aagacagaat gttgtttttg
    46381 ttttgacaag tctctctata atcttttttt gctctctttc tatagtgtaa aaatcatata
    46441 ggttttcaag catgtgtata ctatctgcat gtcttttaat ttcctctgtt ttgtccttag
    46501 aaacgttttc ctgtctaatt atcatttcat tgtatttctt gatagcttgt aacggtgtac
    46561 cttcttcatt tggtaatgaa accactgaaa aattaaattc ttttaaaact tctaaaacat
    46621 catcaaaagt ttcttttaaa gtaattaaat aaacatagtt cattaacttg tcagaattga
    46681 caatcccaat aacactttct ggtatttcat cttctaattt tactcttaaa gcatcaatgt
    46741 ttgcgcttat gggcacagtt ccaagtatag tttttgtata cttagtttcc atattttcaa
    46801 gaggaatatc aaagttcacc catggttcta acatactttt attgttgtta agtaagttta
    46861 tgttagacct tattgtacct atctccttgt gcaaattgtt taactccaga gcttcagcat
    46921 aaacttgccc ttctttttca ctatcaattt tgttaaactc tctttttggt gcaagtaggg
    46981 attttttttg tttcactagt gaagaaacaa tattaattgc ttggtttaca tgagacattt
    47041 tctgctctag cattataact tcagattctt cactatcttt tacaagataa ttttttaatt
    47101 cctcctcctc agtaagaaag gcactatcat caatctgaac aaacccacgg ttcataagta
    47161 gctttaaaat ctggcttttt tgagcttcta agccaacaat agatattttg ttcatattaa
    47221 ctattgccat attatctcac tacctttcca ataaccgctt caatagcctt actttttctt
    47281 tcttcagcag ttttttcaat tttcagacat tcacttttag cattagctaa gattttatca
    47341 acctctgctc tactgtcggt gactgccttc tcaataagag tttcttccat aagctttgct
    47401 tgttttttta catcagcaaa tatgctttca catttgttta cagcatcagc tctgatagac
    47461 tctgcatcag taacagcttt tactttgata ctgtctgctg tctgctctgc ctcaacaatg
    47521 ctttttatta tttcaaaagc catccaaatc acctctctta cctttttact atgtatataa
    47581 ccaattagat ttttttattc taattagatt actacctcat tgttaaagta taaacagcat
    47641 ttttatataa taattaatat ttttcaacgt ttatttctta ataaaacata ttttagaata
    47701 gtacttgcct agatttacat ccttttttga cttactttta gactatgaca tgagtatggt
    47761 ttttaaatac cattattttc aataattata atttaaagcg atatagtttg tcaatagcgt
    47821 tattttgttt taaaattaac gttatgtcga cattttaaat ttttatttta ttttttttat
    47881 aattttaatt taatttatta catataaaag ttttttatat tctttttcaa cctctaacca
    47941 ttgaagttta ttgttttttt tgaattttta gaaatatctc gcactatttt atctaacttt
    48001 tataaatcat caagctatat cgatttttgt caccttattt ttaatctaat ttttttatat
    48061 ctagttagaa ataaatcaat atttttaaag attgataaaa cttacaccta gttctaatag
    48121 ttttatttta aggcaatgta ttaatcttaa gtagataata tcattagtta ctctatatat
    48181 tcgacaacat tataaaaaaa tcctcgtacc aatcactcca atcttcaata ggatatgctg
    48241 ctcctccatt tacacttttc agtagattgg atgtatattc ttctcccata gaaaaaagtt
    48301 cccctactgt catatcataa cctctagatg taacaacctt acaaaatgaa tccataacat
    48361 cacgttgttc tcttgttttt ataagttctt ttctaaattc atcatcattc ttagcttctt
    48421 ctaaaatttt agttaatttt tcattcatta tgtatttctc cttttaagta gtctttctgt
    48481 aaacttcaat aagctttata taaaaaggaa gccatcctaa ataaactaag atgacttcgc
    48541 ttaactgtta gcaacacatt tactagtata aaataattgg tgtacctggt tctatatttt
    48601 catatatttt ctttgcctta gaaaaaggtg tatttacaca tccatgtgaa cctgaagact
    48661 tatatatact tccaccaaaa gaatttctcc aagtagcatc atgaatgcct atgttcccat
    48721 taaacggaag ccaataactt acaggtgaac tgtaaccttg accagttaat gaaacatttc
    48781 tagccttata attaagagga tatatacctg atggtgtagc aaatcctcta gctaaatcac
    48841 ctgttacaac gtctgttgat acaagcattt ctccattttt aaagaaccac atatgttggc
    48901 cagaaagact aatctctaca tatgtatttc caatatcatc tttattacga gatacagctt
    48961 tttgtatgta aataggttct cttgtaacat ttttaccagc ttttacaaca tcaactaatg
    49021 cttcagcttc tttcttttta tctatcaacc aaccataaat tccacctgaa attgtaatat
    49081 ctttacctgt ggcagtttta aatggtcttg agtcaccaaa agtactgtat tttttactta
    49141 aacctcttgt atattctacc atagcatcat tatcaaaagt tatgtcataa ttcttttctg
    49201 agtctacttt taaccattta ttaatttcag aaccatctaa agtttcctta cggtcactaa
    49261 aatcatatgt aacgactaca ccaacatact tatctaatgt cttaatagct tgtttcaact
    49321 tttctgaatc ctttgtgttg tttggtgctt cataaacttt actttcatcc agagaaatag
    49381 aagtattccc agaattaatt gagttttgta ctaacttata aaaatcatct tttttcactt
    49441 tgtttccaag tacctctggt actataacat aagcgttttt agtgctatca tacttaggat
    49501 aagcactctt aggttctaca acatttttac tatcaaaaca agataaagca ttaaaccttt
    49561 tttctagtaa atctttatta tatgaaagag ttatcatatt attatactct ttagacttaa
    49621 atattccacc aatccatcca atagcgctct gactatcttt tatcttttga actttatcat
    49681 ctggtgtaaa ttttaaatca atatcttttg cttttatttg ttctttctta ttgtttcttt
    49741 cctctaagtt taaagtatag gaattagtac tagatgttaa caaatcattt acttgctcaa
    49801 ctgtttttcc tgaagccttt acgccattaa tagtcgttcc aaagtaaaaa tgatttctaa
    49861 aaaagattgc cattcctata taagctacaa acaaaacacc tactactata gctatcttaa
    49921 attttagatt tttttgtcca ctttcttgtt ttacctcttt tccatcaatc ataattaaat
    49981 ccttccttac attgtaatta cctatgtacc ttattggttt ttaaacaaaa ccatgtatgc
    50041 cttattttat atacccaaat tactgtatca ttatttatat ttttaaatat atagttattt
    50101 ctataaaacc attttatctt ttagataaaa tgattattat aaatcccccc tttcaaaatc
    50161 ccttaaacac aatctctgct atattaatat attaccatac ttttaataat cgtgggacta
    50221 tctatctatt tttgtaacac taaactacta ttttaaaaaa gaaactaatt ttgtgttagt
    50281 ttctttatct taacttattt ttatattgaa ttttagctta acataacgtt gttatataga
    50341 ttctatttaa ttattttagt ttctatatct tttccatctg catattcttt aagtaattcc
    50401 ttagcatatc caaaatattc ttcaatatca gattcttgac taaaagaaaa tatacaaatt
    50461 aatatttcat ttgcatcttc tgttatcatt gctctttcag aatcactagt tggactccca
    50521 aatttaccca ctgaatcaga taatatagga agattttcta tatttattaa ttctttccca
    50581 atacccttat actgttctcc ttcatctcct acagtaaaac atactggtga atgtaatttg
    50641 cttacattat atgtacctac tggatataat gattttattg atattaaatt gtttatttct
    50701 actatattat ttattgtata aagacccttc ccttgaataa ttcttctaac caatgcttct
    50761 gaagatgttc tatatctact tggatttttt ccaagctctt tataagcatt tcttgcatct
    50821 ttaattttat ctagtttcgt aatgtcttca acttttagac tttgatttat agtatcacaa
    50881 aactcattta tttcattaat taatttttct gagcttttat ttactgctac ttttgcttga
    50941 atgcaaccca gcttagcatt aggccatctt tccttaagtt tgtcatcaat acttatatct
    51001 atcatactta aacaactcct atacactaaa tttttattaa agtttttatc atctatacat
    51061 tatagtacta ttcttttgtt aataacatgt ttattagcaa aataaattta tttttaatat
    51121 aatttttata aaatacaatt attttttgct ttatattaga caatacattg ataaaataaa
    51181 aaaacactaa ttatttagca atgatttatt aaaatcaagc attcataata gtgctttata
    51241 taaaaatcaa attttgtttt aaagtaaatc taaaaaattc ttagctggca ttggtcgtgc
    51301 aaaataaaat ccttgaacta aatcacatcc aaattgtttc attaactcag cttgttcttt
    51361 actctcaaca ccttctataa ttacaactaa atctagtgat tttgctagag acactattga
    51421 ttttgctata ttgcgacttc tctcatcatg ttcaatgcca ccaagaaaag acttatctag
    51481 cttcaatata tcaattggca tatctcttaa acaactaata gaagaatatc ctgtaccaaa
    51541 gtcatccata gaaatagaaa atccatacgt cctcaactca tttaaaacat tctttaaaat
    51601 atttaactca ttaatagcca cagtttctgt tatctctatt tcaatgaaat ctgaacttaa
    51661 attatacatt tttattgttt tattgataaa ttttacaata tctggttgat acaactcgac
    51721 cctagataaa tttactgaaa ctggaactat ctttttacct tgctttttcc attcatataa
    51781 atcattgcaa accctttcaa atacaaatct cccaattttt aaaatcaact tacttttctc
    51841 tgctattgga ataaatactg cgggacttat aaaaccatgt tctggatgat tccatcttat
    51901 tagtgcttca ctccctatca tttctccagt tacaagtgaa aacttaggtt gataatacac
    51961 ttcaaattga ttttttacca atgctatttt tatatcatct aaaatcattg attcctcact
    52021 aagcttattc ctaacatctt cattataaat agcataattt atatttttac ctttagcaac
    52081 agacctagcc atatttgctt tatttacagc cttttgaata tctacttcac cttcttcaac
    52141 aaaataaata cccacacaca gagaaatttc aaagactata ctcaaattac tttcaatatt
    52201 acttcgtata ttatcaagtt ttcttataat tttatttctt ccatttttat aatcacagaa
    52261 aattgcaaaa taatcacttg aaagtcgtgc atatacctca ccttctgtta attcttcttt
    52321 taaagctttt gctattgtaa ttaataaatt gtcaccaaat tcatatccaa aagtatcatt
    52381 aatcatctta aagttattaa tatcaaaata tactataata taatttaaag atggtttatc
    52441 taaaagatac ttactacatt ctctataaaa tttattttga tttcctatat atgtaacttt
    52501 atcttcataa gccaaactta atatttcttt ttcattagat ttttttatat aaattatata
    52561 aaaaataatt gcaataaata ttaatacaat agataacact gcatatacag ctctgttgat
    52621 tatttgtttt gaattactaa atacaacagt ttctggaatt gaaaaaatta aattccagtc
    52681 ttttatataa ttattttcac tattcccatt atccatagtg gcatatccta aatatcttct
    52741 ttcttccaac atatttaata cagttgcacc tgttttattt tcttgcaatt ctttttttaa
    52801 tctatttact tcttgaatat cattatcctg ttttaacaat ttataaatat tttttgatag
    52861 gctatcccct tgtggatgaa ggataattgt accattattt tttacaacat aagatatact
    52921 attattttca taaaaagata tattaataag tttcatcaga gtattgatat tgtaagtaca
    52981 atataaaact ccaactactt tatcattatt taataatggt actgaaatag caatcttctt
    53041 tatagactta tttacttcat caacatatgg acttgaaaca tacttcttac ctttaattga
    53101 gtttttgaaa aattctctat ctttcactga aaacttatca cctttggtat tttcagcatt
    53161 accatttata tcaactattg ccatagtgta aaatttttga ttttccattt catctaaaag
    53221 atttttcatt ttttcctcat taggtatatc tggatttgaa gcataatagt tagaaaaaat
    53281 atttaaaaca tctaaatctt tttcaatttg tctttgtgct agttgagagc cttgtactgc
    53341 aacttctttt atatgtattt ttgcctctga cataagaaga tgttttgtat cttctatata
    53401 gatatacccc attgtcaaaa ataatgatgt taaaaaaaga attgtaatta atgtaaaact
    53461 tttatttctc cctgtcattc gactcctccc ctaattagtc aatacttcat atagataata
    53521 tatccttttt ataccaattc atcatttata ttgtatataa ctattcttaa atgttacaca
    53581 taaaatcaac ataataacca taaaaacatt cttttattat tataataaca ctatttaatt
    53641 gaattttaaa ttaaagaata aaaactatcc attttctcaa acaaaaaaac tcttatacaa
    53701 taaaactttg ttttatgtat aagagttttt attttatcta ttttatagca aacttatctt
    53761 ccataatatg aatttaaata catatctttt atttcattca taagtggata tcttggattt
    53821 gctcctgtac attggtcatc aaatgcttga attaccattt catctattga atccataaac
    53881 ttagattctt caacgccaaa ctcttttata gatttaggaa taccaacttt aagttttaaa
    53941 tcttcaatag ctaccaataa actttggaat ttctcttcat cagaatttcc ttttaatcct
    54001 aaaaatgaag ctatttcagc atatctcttt aatgcttctg gatatttata ttgagagaat
    54061 gctcccattt tctttggtgc atctgaagaa ttaaatttca taacttctgt tatcaaaaga
    54121 gcatttgcaa caccatgtgg tacatggtgg aaagcaccta atttatgagc catagagtga
    54181 caaacaccca aaaatgcatt tgcaaaagcc atacctgcca ttgtagaagc atttgccatt
    54241 ttttctctag cttctgggtc ttttgcacca ttatcatatg cacgtggaag atattcaaat
    54301 atactcttaa tagcttgtaa agctaatcca tttgtataat cggttgcaag catagataca
    54361 tatgcttcta atgcatgagt taaagcatct ataccagaag cagatgttaa gctttttggc
    54421 atattcatca tcatatcaga atcaactata gccatatttg gcatcagttc ataatctgct
    54481 aatggatatt ttactcctgt atcttggtca gttataacag caaatggtgt tacttctgaa
    54541 ccagtacctg ctgatgttgg tatagctaca aaattagctt tttcacccat tttagggaat
    54601 gtatataccc tttttcttat atccataaat ctcatagcta ggtcttggaa atctacatct
    54661 ggatgctcat acaatgtcca cattatcttt cctgcatcca ttgcacttcc tccacctata
    54721 gatattatta catctggatt aaattctctc atagctttag cgcctatctt agcacattcc
    54781 aaagttgggt ctggttctac ttcaaagaat gtagtgtact taatattcat ttcatctaat
    54841 aaatcagtaa ctgctttagt atatccatta ttgtaaagga atgtatctgt tacaataaaa
    54901 gctctcttct tattcattac atctttaagt tcttttaatg ctactcctaa acaacctttt
    54961 ttgaaataaa ctttttctgg tgctctaaac caaagcatat tttctctcct ctcagctact
    55021 gttttaatat taagtaaatg cttaactcct acattttcag atactgaatt tccaccccaa
    55081 gacccacatc ctaaagtaag agaaggagct agtttaaaat tatataagtc ccctatacca
    55141 ccttgagaag aaggagtatt aattaaaatt ctacaagttt tcatggctga agtaaactta
    55201 tcaagttttt ctctttgatt tacatcatca atatataatg aagatgtatg cccaaatcca
    55261 ccatcttcta ctaatttttc tgcttttcta actgcatcat caaaatcact tgccttgtac
    55321 attgcaagta ctggtgaaag tttctcatga gcaaaagctt cttctatttc aactgattct
    55381 acttcaccta ttaaaacctt agtatctact gggacttcaa agcctgcaag ttttgctata
    55441 gtacaagctg tttgcccaac tattttagaa tttaatcctc catttactaa aattatattt
    55501 cttatttttt ctgtttcatc tttatctaat aaataagcac cacgctcttt aaattctttt
    55561 ttgacttcat tgtatatatt ttctaatact ataaccgatt gttcagaagc acatatcata
    55621 ccattgtcaa aagtcttaga tacaagaatt gagtttacag cagtttttat atctgcacta
    55681 tcatcaatta ttgctggagt attacctgca ccaactccaa tagctggttt tccagatgaa
    55741 tatgctgatt taaccattcc aggacctcct gtagcaaggg tcaaatccac ttctttcata
    55801 acagttgtag ttaattctaa agatggctca tctatccatc ctattatacc ttcaggagct
    55861 ccagctaaaa cagcagcctc aagaacaact tttgcagcct ctatagtaga attttttgct
    55921 cttggatgtg gagagaaaat tattccattt ctagttttta aagctaaaag tgctttaaag
    55981 atagcagtag aagttggatt agttgtaggt acaactgctg caataacacc tattggttca
    56041 gcaattttag tcataccaaa tgcttcgtct ttctctataa caccacaagt tttagtttct
    56101 ttatatgcat tatatatata ctctgaggca taatgattct ttataacctt atcttcagca
    56161 attcccatac cagtttcttt ttgtgccatt atagctagag gaactctttg cttattagct
    56221 gccaaagatg ctgctaaaaa aattttatct acctgctctt gactatatgt agcaaatatt
    56281 ttttgagctt ctctgatttt tgctagtttt ttaactaagc tttcaacgtt atttacagta
    56341 tctaaaccaa ctgacataac ttcattttct tttttatttt ctattattac ttgttcttta
    56401 tgagccattt taacaacctc ctgagatagt tttttattta ttatgttaat ttattaacat
    56461 tatagcattt acttttccta ttttcaagtt atttttttaa cattttattt tttatctaat
    56521 ataacatcat ttctactaat aatatctagc tttacaaata cttaatttaa ctagatatca
    56581 ctttattttt tacgcaaatc actaatgaaa tcatctaaaa acttaaggat aacgttattt
    56641 ttttaacgtt tatatttttg tattttttgc aaataaaact ttataaaaag aatttttaat
    56701 atgatactct atcaagttta aaaataaaaa tccttgaaaa tgaaaattaa aacttaatta
    56761 acaagttaaa gtaaatatag ttaaatgtac aaacatttac aacatatagt ataattaaaa
    56821 aaatatatat tcatgatttt tatataatat gaaataaaat atatttaata ttatatagat
    56881 aaatttgaag tttataagaa ggaggataag catggaaaaa ttaaaaacaa ttagcgtttt
    56941 ttctttgatt ataagtgtaa ttttaacgat tggaggtatt ggaatagtaa cttattatgt
    57001 agataatttg tttattagag gactatcagt atttgtttta attatgtcct cttcttttgt
    57061 atcaacaaca gttagattga tatttgaaga gtcaaaacga tataaattct aatttaaaaa
    57121 ttggattaag taaataattt taatatagca aaaattacgg agattagctc aaaatagact
    57181 ttttagtcca tgaggcattt ttttgtatga aattaaatat attttcatca tttcaacaag
    57241 aaaaagaggc ttctctctat tttgagacag cctctttgct agtattgtat ctaatacata
    57301 atattataag aaccaattct attgatgtgg actttttata acaggctgta gctgtttgtt
    57361 ctctagagca agttctatag ttggaattat tagctcagta tgtgcaatca tagaattagg
    57421 ctttgctttg caattatctt gaccaaaagg tacaaaatat atatttttag aattaaggag
    57481 aatgccaata tttttgaaat taaagcttag agcatcattc gttgatattg atattactag
    57541 tggtttatca ttacgaagat gaccttttgc tgccattaat actggtgaat ctgtaatacc
    57601 atttgctaat tttgcagcag tatttccagt acaaggagct attattataa tatcagcaat
    57661 tcctttaggt ccaaatggct ctgcctcttc aatcgtaact ataggtttat ttcctgtaag
    57721 ttcatatgct tttttcataa actcttcaga atttccaaat ctacaatcga tattttgtga
    57781 tacatctgaa aatatagtgt gtacattagc tccttcttta accaaattct ctaactctat
    57841 aaatatttta tcataagtac aaaacgaacc agtaaatcct actccaattg taagtccttc
    57901 aagtctcatt tctaatccct tctttcattt ataatatttt ctatagcgtt aacaagaatt
    57961 tcaccagatg tctttggtga atatttacca ggaagcccaa ggcaaagagt cgcattaatc
    58021 cctgcttctt taacaacaga ataatctact cctccaggag aagaagctat atctataata
    58081 gttacttctt gtgatacttt ttctaacctt tctttcgtta aaatacactc tggtatggtg
    58141 ttaaaaatat aatcaaattg taaaagtttt tcatcaagtt gacttaaaga aatactggaa
    58201 tatccaaatg cattagccgt acttcttgca tcattatttc ttgcgccaac acatacattt
    58261 gcatccaaag cgtataattt ttttgcaagt atttgtgcac atcttccata acctaatact
    58321 aaacagttac tttgatgtat attgattata cttcttttaa tagcttctgc tatagcccct
    58381 tcagctgtag caactgcatt taaaatagca acatcattca ttttcataaa atcataaatc
    58441 tgaatagagt gttgctcgca aaatttacaa actttagttg gtatgttacc tccaaataat
    58501 atttgattag gagataaatt aattagcaag ttatcaatag tagcatcaag acaatcatcc
    58561 gtattttcaa tattaacttg attctttgta aatggaattg gacataaaat aatggaactt
    58621 ttggatagtg cttcagctag tgaagttgat tttccaacga ttccatttat aatttcattt
    58681 gatatcccat aagtgactac agaataccct ttattaatta gtttttttac catgtacact
    58741 tgtcgcaggt ctcccccaat aacagttata tcgtattgat ttgacatatt gtaagcctcc
    58801 tttactatat acaatatgtt tttatcaata cattggttca ctaaaattac taaatattat
    58861 acttataaca tttttaataa tgctaatcta tctatctttt cagaagaggt atatggaaat
    58921 gaatttaaaa agataatttt ctttgggatt tcatttttca ttagattatc tttcatttta
    58981 atcaacaaat ctttcttcgt aattttgtct atagttatta caaataatac aatttgattt
    59041 cctcttatag tgtctgagta cggcaataca attgcatctt caatttgagg aattttttta
    59101 acttcatttt caatcttagt agaactaact ttaaatcctc caatatttat aatgtcatca
    59161 cttcttcctt caaatattaa gtatccatca ttatcataat atccaatatc attgacagaa
    59221 taaggtttgg ttgcattata tactgcatat tctgtattca caaaaatttt tccttctcta
    59281 atatatatat ctattcctgg aaatggtctg cctacactta gaggtttttc aattagttca
    59341 tcatagcata aatatgtaat ataatttaat tcacttgccc catagtataa aataatttca
    59401 gactttggca tatatttttt tagatttctt gcagtatctt caaataataa ttgagaccct
    59461 gtaaaaatac ttacaacttt gagtactggt tcttttaagt gtttaaccaa aagttgaagt
    59521 tttgtaggta taaggtatat gttagttaca ttatattgtt ttattgtcct tatccatgat
    59581 ttacaattaa gacctgaact tattacaata ctacctcctt catataatac tgacattata
    59641 gaatttagat tccctgtaaa actaagtgtt ccattaacaa acaagattga ttccccagag
    59701 atattaaaaa taccattttg tataggaaaa aatccagccc aactctcata tgttctatat
    59761 aatacctttg gaacacttgt agaacctgaa gataaagctc ccatacaaat acttttatct
    59821 aaataattta taagcttctt atattgatac atatatatat taaatttagg attaaaaata
    59881 gaaaaattaa ctttttttgc tacttcatta ttgttatcat catataaatc aataacgtcc
    59941 atagattcat cagatataat gattgaaata ttatttttta ataaaatatc atctaatact
    60001 tttttagaaa gattataatg acaaataata ggaatatttt ttgaataatt aatagcaaaa
    60061 aaactaatca actgaaacat tatattttta gaatatatta aaattgggat attttcacct
    60121 aaagaaattt gctttcctag ttcctcacta taactcaata tattttcata agtatatttt
    60181 tctccatcta taattaaaaa atttttattt ttatgcctct ctttttttat tcttaaaagt
    60241 tcataatagt tcataatcta caccctttca agcagtaaag ctgaacctag accaccagct
    60301 gcagctatag agcaaattcc atatctaccc ttagtctttt ctaatgcttt taaaagatgt
    60361 aatgcaataa ttgcacctga agcaccatat ggatgtccat acgctaaagc tccaccaaat
    60421 atattatatc tatctattaa ctctggatac tttctttgaa acaacacatc tataactgca
    60481 aatgcttcat taaattcaac agcagataca tctaaataat taaggctttc tatctctaaa
    60541 agtttatcca ttgcttgtat cgctgatgtt ggacttaagg ttgcatctgt tcctatcgtg
    60601 catgtgttaa taatttttgc tttaggcttc ttttttacat gttctaaata cttcttagaa
    60661 catattataa taaatgaagc tccatcatta ataagacatg aatttgctgc atttgtaata
    60721 gtttcttttc ctaaaattga tggcatccta tctaataacc tttgactcat cttatcacgt
    60781 ataccttcat cataggtact attatttata ggagatataa tatcttcaag tattttttcc
    60841 tctcttgctt cttttgctct tttatgactt tcctttaccc aaaaatccaa gtctgctttc
    60901 tcaatttgat ataactctgc tactctttcc gcaccttcga gcatactatt ttggctatta
    60961 tcatcaggag aaaattgagc aactgtataa tttggattat ttgtattata tcgcttatca
    61021 ttcttatgat atgtgcgcat tggttgcaga gaactacttt caaaaccacc tgcaataatt
    61081 aaatcacact gaccagattt aacctttgaa aaagctatgt ctatactcat catagcagaa
    61141 gcacactgca tatccacagt aaaagcaggt acttcatttg acactcctgc tgttaaagtc
    61201 attaatctag ctatattccc tcctgtccca actgcatttc cacatataat ctcatcaatt
    61261 ttgtcaattt catacttttt aattaaatct tttaaaacac ttgctcctag taattcaggt
    61321 tgaacaaatt gaaaaattcc attttttaga cctatatgac ttctcaatcc tcctaatata
    61381 aaaacttcct tcatacatta cacccttcct tatattatag atttatcata gatttcttct
    61441 gagtacattg ttcagtttag tgcctaaaaa agatgcaata actgacttta ttgtatctcc
    61501 aattaaaaat ggtactactg ctgcaaaaag tgctgcttct actgtcattt gattaaaata
    61561 gcacataaat actgttccca taaaatatat aataggcatt ccaacaaaaa ttgttattaa
    61621 tatatatctc ttaatatcat tatttcgtcc ttttaataaa ctaataatta gtgctgcaaa
    61681 caaaaaccca aaataaaaac cacctgttgg gcttaataac tttccaatac cagatgtacc
    61741 tccaccaaat actggaatcc caattaaacc tatcattata tatacaccaa ctgtactaaa
    61801 tgcttgtctt ggtgttaaaa tcaaagctat taaatttatc attattgttt gtgctgttac
    61861 tcctgctgga gtaaatggca atggtatata tatgtaagat gatatacaca ataatgctac
    61921 acatattgac attttagtta aatcttgaat atttacttta gtatttttca tttttataat
    61981 tcccttcata tgtcttattt catttagttt atatgtacat atacaaagct tctattaaag
    62041 ctttgtttta gtataaaata aagacgtttt tttgtcaact attataatta tttaggttta
    62101 catttaattt ataagtaaac tttatactgt tactttatat tttaatagtc atttataaaa
    62161 ctttgcttaa aaacagctct atttagacta tacttgtaaa ttataatatg atatattttt
    62221 atataagatt ttattttata taaaaaatag taacaaatag tttatacaca agatatttaa
    62281 aaggaagtga tgatatgaaa gttaatgaaa ataatataga tgaagagtta gatgaactta
    62341 gtaggaaagt ttacttagag tcaccagatt tttggataag tatttctgac aaaataatag
    62401 ataaagtatt tgatgaaatg ttattatttt ttgcagttaa atataactat ttcaatatag
    62461 tatgctttgc tattgaaaac aattatctgg atttaaacat gccttcaaaa aacaaaaaat
    62521 acactaatat aatggagcat ttgttagata catcaaaaca gaatgaagat aaaaaaattt
    62581 ataattatct tctaagctta aataaaggta ataacctaga tgaaagacca attttagatg
    62641 aattaaactg taatattagt agtgatactt ttgaaaaaaa agatatatat cttccaaagt
    62701 acttatgtcc aagttgtaat tctaatatat ttgatacagg ttataaagta tcaaatgaca
    62761 taacatataa attttcttct aaagaagctg aacctattga aatgtgtagt gaagtttctt
    62821 ctatcagatg ttgtaattgt gatgaattat tagaagatat tacacttgat aaactatata
    62881 acctatgcaa aattcaaaac tgtaataatt gtggttctaa tttaactaag gtaggaatta
    62941 ttgcaaagaa aaaaatgaac tttgatgaga atactgaaaa atttaaagat gtctcaacat
    63001 tttactgttg tggaaattgt gactctgaaa taagcttatc tcaaagagaa cattttaaga
    63061 tttaaatatt tataggctaa agtaataaga ttacattatt agatactgta taagtttttt
    63121 aaatctgtaa aaataaatac acttataaga attaaaattg ctatgaaact agttcttgat
    63181 gaactaattt catggcaatt ttctataatc taataattat taaattttaa caaaccttta
    63241 ttagcattcc ataattattt aaaattcttt caatactcta tagtttatag gtgaaagtat
    63301 ctttaatatt atatcaaact tattatcttt aaatagtaaa tttgttaaat ctgaaataca
    63361 ccatacaatt ttattttcct cttttgtagc tatatcaatt ctgattaact tacatctata
    63421 ataaatatct ttatcttcat atagtctatt ttaaaaatta ctcttaaaaa atcaactaga
    63481 aaataaatta attatccttt taatagaatg taatcatatt atttactata ttccattatg
    63541 tactctttaa atcctgaatc ttcatttact tgttctttta ctatattaaa acctttttta
    63601 ttataaaaca caactgcttt tttattatct ttatatactg ctaagcttag acttttatat
    63661 tttttagttg cataatctaa aagtttacct ccgataccta gattttggta tttgggcttt
    63721 ataaataaag ctcctataaa gtttttatct attatgctta taaatccttt tatttcatct
    63781 ccatcatcat atacaaatgt atctgatata ggtatatatt cgtttttaac agtattatag
    63841 ttattttgcc agtattcttt acttataaag tcatgtgctt tgattgtact tttttcccat
    63901 atttccatga ttttatttat gtctttactg tttaatttct ttatcataaa ttttgtttca
    63961 cctcattttt gtattgattt tataatgttt gtaggtaagt gtattttatt tagatatgat
    64021 ttagttaata taaggtggat gttttagtat atgctattgt tgattctaca attttatata
    64081 ctgctttatt aatataaaat aagtttctaa atactcgttg atattgctaa tattctactt
    64141 atctaccact tagttgatat aaaaattgaa acttcactat ctaataattt aattaatgat
    64201 atgctattac atttatctat ttcataacca ccataaatcc caggtgttga atttataaat
    64261 ataccagctt gctctaaata catcttgtaa ctttttattt gcctttcact tacttccaac
    64321 tcttctgata attctttgca tttcattttg cctctgcttt gaattaatat catcattcta
    64381 agtgtattag ctaatttact aatgagtaac ccccccctaa taacattaat tgatattttt
    64441 attatttatt tctacacata tgtacatata acctctttat attttatata agtagaattt
    64501 ttttataatt ttgacattta ccatattata ttttatattt gt
//
LOCUS       DAMJYR010000006        64185 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.006, whole genome shotgun sequence.
ACCESSION   DAMJYR010000006 DAMJYR010000000
VERSION     DAMJYR010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 64185)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 64185)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..64185
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.006"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(462..2528)
                     /locus_tag="PC471_000400"
     CDS             complement(462..2528)
                     /locus_tag="PC471_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HDF2787542.1"
                     /translation="MVELIITTNIDIRKLNENQLEAVEHIDGPCMILAGPGSGKTRVI
                     TYRIANMVVNKNIAPTRILAISFTKASSIEMKNRALNLSDDIRLNKVTYGTFHSVFFK
                     ILRYFERYNLDSIFDEKSKRMTIKAILKSLNIENADDDENIGQVINEISYVKNELMDK
                     NEFNSEVLTKDEFLKVYNLYEEQKSKVNKIDFDDMLIRTYYLLLNNKSALEMVRNVYK
                     YILIDEFQDINKVQFEVLKLICSPLNNIFAVGDEDQSIYGFRGARPDFLLEFEQYFNN
                     TKKIILDINYRSKSEIVHTANRLIDKNKNRYEKIIKCSQGDGGSVTYISPHDSEEEAL
                     YIAREIIDEIQKDYVEYSDFAVIYRTNIQSRALVDVFMDMRIPFVVKDSVITIYDHWA
                     SQDILAYLRIGINPKSNKDWLRIINKPFRYISKDSINMVKDEKDFITALINKCNLHPK
                     QVKTINDLEIDLSYLNTLNPKNAISYIRTSLDYDRYVLDYCSNRKIKTNGLVEILNEL
                     ESSATNFNTVTEFLEHIDRVKTELTENNKNKQTEGVIFTTMHSAKGLEFRNVYIIGVN
                     EGTIPHEKSYDICKEEKKEEQLEEERRLMYVAITRAEEKLCISSTLNKYGKKVDKSLF
                     INDIKSPTKKEIDSIGVGDKVYHKKFHEGEIIKKDGIMFTIKFKDRERILDLKTCLLK
                     NIIYTI"
     gene            complement(2605..5634)
                     /locus_tag="PC471_000401"
     CDS             complement(2605..5634)
                     /locus_tag="PC471_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HDF2787543.1"
                     /translation="MENNLRKNNSIMKKMIFPLIIVMLIQTSLFCATILWGGTIKKLN
                     DNSFDILNERVINRKNYIQNEMLQRWSNTSETEATINSSVKKVLNEKNALIEDVGNNE
                     EINKDIIKSISNDLIYLLRKNSVTGAFIILSDKNINDKSIDSKGEISRTGLYIRDLDP
                     KFNPNDNSDLLIERGSSDIARSFGISMDSYWEPKFSFKSENEQDGDDFFYKPFRAAID
                     NPNVSSSNLGYWGSKFLLTNAGDRLMTYTVPLIYEDGTVYGVLGIDLSVNYLRELLPY
                     SEINGNKKGNYILGVDRNNDMAFENVISNGYSLKEKFWDGLHSNLEQKESYKNIYKIK
                     NKDNECGNGNIYASVQYLDLYNSNTPFENERWALLGIIEEKSLLSFSKRVEKLVIISI
                     AISLLIGLIGIYIASRLFTKPIVLLANKVRNSNPSKPVNLDKINISEIDELSSAIELL
                     SANVADSSSKLSQIIEMVDMPIGAFELYKNAENVFCTKEFFSVFTTEIKFEEYGYMEK
                     NKFLKKLRGLDKYLQESYETENTYIYKFESKDKKLKWIRMNIFEDELKVLGVVVDVTK
                     EFIEKRKIEYERDFDILTNLLNRRAFHSQIQEKFKNKDELKIAAFIMWDLDNLKYIND
                     TYGHDAGDEYIRCAADILKKFISWNGIVSRLSGDEFYVFIYGYESKDSIRDIINSVKK
                     QMNNTLLKLHDGTEFKIRASAGISWYPDDSTNYDELMRYADFAMYQIKNTIKGEVSEF
                     DINIYNKDSFLLSNREELNKLIDGQLVEYAFQPIVDCKGKVFAYEALMRPKIKTFSSP
                     IDVIRLARSQSKLYQIERITWFKALKSFKKYKEKFGDCKLFLNSIPNNNLSKVDFEEI
                     EDLYGEYLNRVVLEITENDKINEDFIEKKKAYIDHWNIEIALDDFGTGYNGDAVLLYI
                     MPDYVKIDMSIVRGIDKDENRQKILKNLISYSNERNIKVIAEGVETKGEMETLINLGV
                     DYMQGYYISKPSFIPEDISDKIKKELKICHENRNDVV"
     gene            complement(5687..7147)
                     /locus_tag="PC471_000402"
     CDS             complement(5687..7147)
                     /locus_tag="PC471_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HDF2787544.1"
                     /translation="MNRCKFAKLIITSMIVLLLTGCSLKKEDNYKLDPENPTSIQIWH
                     YYNGQQKMAFDKLVKEFNDTVGAEKGIIVEAFSQGNVVDLSEKVLDSANKKVGSEDVP
                     NIFAAYSDTAYEMDKLGLLVDMDKYISKEELNEYIDEYVEEGRINSKDEFKLFPIAKS
                     TEILMLNKTDWDKFAKATNSNINDIKTIEGLVETSKKYYEWTDSLTKEPNDGKAFFGR
                     DALANYIIIGSKQLGVEIFSVKDGKVTLNIDEKVMKKLWDNFYVPYINGYFASYGKFR
                     SDDAKTGDILALVGSSSGAAYFPDRVMLNDNESYNIETSVFQAPRFKGEGKYAVQQGA
                     GMAVTKSTKEEEYASVEFLKWFTDKEPNIKFSIASGYLPVKKKTNDLDVIKEVMKKDN
                     LSLSDKVEGSIASSIEQINSCNLYTNKAFDGGTKARQILEYSMLDKAKEDSNKINELV
                     KSGTSKKDAVAQFNNDKNFKTWFESFKAELENAIKK"
     gene            complement(7239..7613)
                     /locus_tag="PC471_000403"
     CDS             complement(7239..7613)
                     /locus_tag="PC471_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2787545.1"
                     /translation="MGNKNKNTKYKDKTRERYNKKVENLNKEKLNKINKQSKKVDKID
                     DIINKSSIVLCVLLVVFSLLYLFKLVQLNIIMILLVITVAIQQGLVSFGAFRNKNYVK
                     ALFSIFVIVCFLIAFKYVVNPV"
     gene            complement(7789..8529)
                     /locus_tag="PC471_000404"
     CDS             complement(7789..8529)
                     /locus_tag="PC471_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HDF2787546.1"
                     /translation="MLYDEDEKEENNERWLLTYSDLITLLMIFFVIMYSMSNVDAEKY
                     KQLSQSLNSAFGGSSGVIEGGNSKIEPVVEPGSNDLDTLQNAKFKKIGEEIQKYLNEN
                     GMANSVSLRVQDRGLVISLKDTILFDTGKAIVKDNSRDKIIQIGKMLNEMNSYMRVEG
                     HTDNMSIKNSEFKSNWDLSVMRATNVVQLLIDNAGIAPDKLSAVGYGEFRPIAENSSE
                     EGRSKNRRVDIVLVDSKYDNVENVSKDK"
     gene            complement(8550..9338)
                     /locus_tag="PC471_000405"
     CDS             complement(8550..9338)
                     /locus_tag="PC471_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein"
                     /protein_id="HDF2787547.1"
                     /translation="MTTLIFFIVGIASLVVAFVIDGGHVGALFVFSSALVVFGGTIGA
                     VGASTPFPVFKKSIKIMLLAFKNKKPNNIENIIYFKEIATKARKEGLLSIDKEISEND
                     ELDPFIRKGLELMVDGVDAVTIRNILESQIQITSKRHKEGIAIFDSAGGYAPTLGIIG
                     TVMGLVHILGNLSSNPDALGPQISVAFLATLYGVGSANLIWLPIASKLKAINQQEVSE
                     RLLVIEAILSILEGDNSNLMTEKLKIFLNSKELIELEEIGGRAE"
     gene            complement(9426..9516)
                     /locus_tag="PC471_000406"
     tRNA            complement(9426..9516)
                     /locus_tag="PC471_000406"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9480..9482),aa:Ser,seq:gct)
     gene            9775..10539
                     /locus_tag="PC471_000407"
     CDS             9775..10539
                     /locus_tag="PC471_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HDF2787548.1"
                     /translation="MLPQERYEKILSILKSDNIIKINDIVQMFDISIETARRDLAHME
                     SIGLLKRVYGGAIPVIASSVELNYVKRKKINSNDKKQIAIKCSEFINTNDTIFMDTGT
                     TVLEVSKNLKDKKNLTVITNSILVANELMNTNIKVYVLGGYLRETEGSFSGPLTLSAI
                     EQFNVDKAILGAGGITLENGISDYHLEEALVRKKMIERSRKSIVVVEPPKFGINSFAS
                     VAPIEHIDTIITSASIDHNIIIEFSQKGIPLVIADN"
     gene            complement(10820..11935)
                     /locus_tag="PC471_000408"
     CDS             complement(10820..11935)
                     /locus_tag="PC471_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutH"
                     /protein_id="HDF2787549.1"
                     /translation="MPFDKIILYIMAVGVLLGALDRIIGNKFGLGEQFEEGFNSMGPL
                     ALGMVGIVCLAPVISDVLGPIIIPIFNLCGADPSMFATILANDMGGYPLAMELAHNKE
                     AGMLAGLVVSSMLGCTLVFSIPVGLGLIEYEDRPFFAKGLLIGLITIPFGGIIGGLIA
                     GFNLSMVIINMIPVIILAVILAVGLKFNSTLMINGSLIFGKFISILITIGLGAAAFQE
                     ITGIVLIKGMTPIREGIQVIGSIAIVLLGTFPILHLLVKVLNKPLTIVGGKLGMDATS
                     AAGLVFTLANSIPVYKMMKDMSPKGKVVNTAWLVCATAALGDHLGFTAGVVPEMITPV
                     VIGKLVGGVLAIALAILMTKDLTLEEEQSIKMKKDAV"
     gene            complement(11976..13502)
                     /gene="glpK"
                     /locus_tag="PC471_000409"
     CDS             complement(11976..13502)
                     /gene="glpK"
                     /locus_tag="PC471_000409"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HDF2787550.1"
                     /translation="MKYVLAIDQGTTGTRAVLINEKSEFVFSDYMEHTQIYPQSGYVE
                     HDPIEIWNNTKTVSNNVLQQAFENGINESDIKGIGIDNQGETVMLWDKNTGKPLYNAI
                     VWQCRRTYDYVENLKNIPGLENKIIDKTGLIIDPYFSGTKIKWVIDNVKGVKEKIKNG
                     EVLAGTLDSWLIWKMTGGKSFLTDVSTAARTMLFNIRDMDWDRELLDIIGVPRSILAD
                     IMPTSGDFGTTDKEAFCGVSIKITASMVDQPAALFGNGCFEPGMVKNTYGTGCFLYMN
                     IGEELNIAGNGILTTVAWKIGDKVTYAYDGGVYIAGAAIQWLRDGIGVIKNYSETDDM
                     ANSISSTGGVYFVPAFAGIAAPYWDQYARGTMVGITGSTKKEHIVRATLESVALQVKD
                     VVDSMNLASGKKIKMLRVDGGITKNNFTMQFQSDILGIPIEIAENPETTVLGVGYMAG
                     LSCGVWNSIDEIRSEFTISKKYIPNMTTQKREEILLGWNQAVKRAMNWENDTKKVCAN
                     "
     gene            13795..14892
                     /locus_tag="PC471_000410"
     CDS             13795..14892
                     /locus_tag="PC471_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HDF2787551.1"
                     /translation="MKKTYLFSPGPVMVTDKVRNSLLHYDICHRGNEFMDLFKDTQQK
                     INKLYNATSNYYSLIVSGSGTSVNECVLSSIFDKEDAVLLVSNGVFGERLEEILSTYN
                     VKTYKPNFEWAEYPDLDILEKYIIENSDIKVIAMVFHETSSGMINPVPELGKLAKKYN
                     KIFFVDAISASAGEYIDVEEFNIDIITGVGGKALGAFPGSAYLCAKESILQNIKENQC
                     KNVYLNLYKHYKVAKSSSQTPNTPNVTLIFALNEALTEFLEDDSKIERYKECSAILRY
                     GMEELGLTFLLPDKYMSNTVTSVFLPKEINVNEFILGLERESGYVVYPGKGKFLDANI
                     FQVANMGEIYPEDCYKFLDILKSKLEIKKVI"
     gene            complement(15383..15895)
                     /locus_tag="PC471_000411"
     CDS             complement(15383..15895)
                     /locus_tag="PC471_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2787552.1"
                     /translation="MKVKKFKSGIVMALTVMAIGASLTNVSAMELNKSKEFETKYNEM
                     KRDFQEEYGKEDKFNITLDDEFTKEEVNGAKGIIKTTNKMNNEVVVLNYFDEMKSGIE
                     FELKPKAEKNAILRDEFQQGYGKEDKFKVVLDDEFTKEEVNGAKGIIKTTDKETGEVI
                     VYNYFDELEK"
     gene            16448..17329
                     /locus_tag="PC471_000412"
     CDS             16448..17329
                     /locus_tag="PC471_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4300 family protein"
                     /protein_id="HDF2787553.1"
                     /translation="MKKRLIIMILSVVLVLSSILTIFAYSNIKYNNNKLIYSNMIDKK
                     TQNSVKEILKENKINEKDIDTFIKSVNNYNKLQVKILQNNINISKSGYSSINAKQVPY
                     NLEKLQDNWVKKFPDYMDVNCRITAFRLFKDFINSNKKFTGDSIDLNVDLDTIMNNKD
                     AKFSTKDVEKFINFFSAIPAKDTDDTIKIAEQIKNEWKKRKISFKNNKNISIINGFLR
                     YPETKNVFIGHTGICIKTKNGILFLEKYGVTSPYQVTKFKNKEDVKNYMFNRLKMSEG
                     EIELPDPIIMENDKLMK"
     gene            complement(17642..18421)
                     /gene="hisJ"
                     /locus_tag="PC471_000413"
     CDS             complement(17642..18421)
                     /gene="hisJ"
                     /locus_tag="PC471_000413"
                     /EC_number="3.1.3.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase HisJ"
                     /protein_id="HDF2787554.1"
                     /translation="MKDGHIHSPYCPHGSKDDFEKYVQRAMDVGITEMTFTEHFPYAN
                     GFRDPAPENDSCMSIEDLPKYFNDVKKLKEKYEGKIKINVGSEVDFIEGYEEGIKENL
                     NKYGKELEDSLLSVHIIKIDDDYYCVDYSVEEFQNLIDKLGSIEAVYNKYYETLIKAV
                     NSDLGIHKPKRIGHLNLVRKFNQVFPYDYKGNKILEELIKLIKEKGYELDYNVSGNRK
                     EYCKEPYIDGYLLDLVKKYDIPLVLGSDSHCAEEINKYDII"
     gene            complement(18967..19317)
                     /locus_tag="PC471_000414"
     CDS             complement(18967..19317)
                     /locus_tag="PC471_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter"
                     /protein_id="HDF2787555.1"
                     /translation="MIFDKEIVRYHHGHHDHDHCHEHTHGDICHEHPHDHAHDHDHEH
                     SHEESSESKDEKTLKILLVHWINHNETHEEGFREWVEKARAIGKEETAKSIEKAIEYM
                     EEANKMLLEAKKHM"
     gene            complement(19333..19749)
                     /locus_tag="PC471_000415"
     CDS             complement(19333..19749)
                     /locus_tag="PC471_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3842 family protein"
                     /protein_id="HDF2787556.1"
                     /translation="MIIAVIDGMGGGIGAQIVSSLREELPTYVEIYALGTNSIATSSM
                     MKAHANKGATGENAIVVSAKKANIIVAPISVIIPNSMMGEVTCNISEAIADSEALKIL
                     LPIMPENVELVGLEGKPLALLVKDSVNLIKKEFNIK"
     gene            complement(19768..19959)
                     /locus_tag="PC471_000416"
     CDS             complement(19768..19959)
                     /locus_tag="PC471_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CooT family nickel-binding protein"
                     /protein_id="HDF2787557.1"
                     /translation="MCESSAFICKSNNELEKVMENVVNIDPCDGKIYLTDLLGEQKII
                     DGIIKEIRLMDHKIIIQEN"
     gene            complement(20110..20925)
                     /locus_tag="PC471_000417"
     CDS             complement(20110..20925)
                     /locus_tag="PC471_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HDF2787558.1"
                     /translation="MNQWRVIHNKSYEGAMNMAIDEAIFTAYKKGHNKPTLRFYTWEP
                     ACLSIGYFQKLEDEIDLDKCRCMNIDYTRRITGGRAVLHDNELTYSIIIGEDNPLIDK
                     SINLSYRYISEGLVKGLNLSGIETDNLNRGERISRENLSAACFNAHASYEVTINNKKV
                     IGSAQSRKDGVLLQHGSIILDFDVEKLFKLIKTKTPELKERAMKFTAKKASGIENEIG
                     RKIDIDILQKNIVKGLAEQFNVEFVEGDLTDYEKQLVKELYEKYKNEEYNKKR"
     gene            complement(20972..21910)
                     /locus_tag="PC471_000418"
     CDS             complement(20972..21910)
                     /locus_tag="PC471_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HDF2787559.1"
                     /translation="MIRLSVGTAIELGILNKKSDIPPTTAYIMIGEKCINKCSFCSQS
                     IESSTRKDKLSRVIWPEFSKEEILDALKAYKGKNIKRICIQSMASEEAHNSVLDFINY
                     ISGKIDMPISLSAKLENDEQIKKFFSAGVDKIGIAIDAANKELYEKIKGNNYDEKLKF
                     ITEMSKSYPNKISTHIIVGMGESHEDIYNLYTYLKENDVTISLFAFTPVRGTKMEKIN
                     QPSIESYRRVQLMSYMINKGYPKEYFKFKNGYLNSIKLDNDILKDINKGYPFEIRGCK
                     DCNRPYYNERPGSTIYNYSRPLNQSEIDLAIREINL"
     gene            complement(21907..22779)
                     /locus_tag="PC471_000419"
     CDS             complement(21907..22779)
                     /locus_tag="PC471_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HDF2787560.1"
                     /translation="MNKNLSEKMKIAFDIKLKNFGNKIEFAYPNQTLALSTTSNQCSL
                     KCAHCNGHYLNNMVPIEEYEEKVQSRNITSFLLSGGCSYEGDVPINTHINTIKNLKEQ
                     GYRLNAHLGLMDKDSIVELCKYLDIVSFDLVFDDETIREVYKMKKSKEDYIEVYNTIQ
                     EHTEVAPHICIGLKGGQIKGEYEIIEYLQKNPPNKLTFIVLIPTKGTEYENVEPPELE
                     GVADILCEARINLPDTEINLGCMRPRGVYRKELDQLSIMCGVNRIVLPSRSAKNKAIE
                     MNMTINECKECCVL"
     gene            complement(22804..24732)
                     /locus_tag="PC471_000420"
     CDS             complement(22804..24732)
                     /locus_tag="PC471_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4445 domain-containing protein"
                     /protein_id="HDF2787561.1"
                     /translation="MIKVSFTPNNKEVYCNEGDILLEVARNADIFIDAPCNGNVSCGK
                     CKVKLLNGKVDTEKTRHITDDEWEQGYILACCTKVISDIEIEVPSKVSSSMHGMKIEG
                     SNKKEDREIFERAKKIIEEHNLQFKTNIKKKYIEMEEPNLDDNISDVDRLERYVRNNL
                     GYNEIDFRLDILRKMPTVFRKSDFKVTITYVQKQKKLTIINIEQGNKENSLYGVAIDI
                     GTTSVVVCLVDLYSKEVVDKASSGNAQIKYGADVINRIIYSTKKNGLETLHKAIVEET
                     INPLLKTIYERNGIDKEDVVTLVAAGNTTMTSLFLGVYTDFLRQEPYIPPFLKSPKLM
                     GENVGLFVNDSAYVYLAPSVASYVGGDITAGVLSAGIWSSEENVLFIDLGTNGEIVFG
                     NKDYMMSCACSAGPAFEGGGISCGMRASAGAIEKVIIDKDTLEPTLKIIDECAPVGIC
                     GSGIIDLICQMITKGVIDRRGKIYRDLDNKRVRFNEHEIGEYVLAFKEEFDLENDIVV
                     NEVDIDNFIRAKGAIYSGAYTLVDSLGMDFSILDRVYIAGGIGNNLDIENSIIIGLLP
                     DIDREKFTYIGNSSLVGSYLALISKDAKNKLEEIGNQITYVELSVYPSYMDEFISACF
                     LPHTNIEQFPTAKKLLEE"
     gene            complement(24844..25221)
                     /gene="gcvH"
                     /locus_tag="PC471_000421"
     CDS             complement(24844..25221)
                     /gene="gcvH"
                     /locus_tag="PC471_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HDF2787562.1"
                     /translation="MKLLPELKYSKDHEWVKVIDGDVVYIGITDYAQDQLGEILFVET
                     PEVEDTVTKGVDFGVVESSKVASDLISPVNGEVLEVNEKLEDEPECINEDPYENWILK
                     VKLADVAELDTLLSDKEYEAGLE"
     gene            complement(25308..27434)
                     /gene="cdhC"
                     /locus_tag="PC471_000422"
     CDS             complement(25308..27434)
                     /gene="cdhC"
                     /locus_tag="PC471_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CO dehydrogenase/CO-methylating acetyl-CoA
                     synthase complex subunit beta"
                     /protein_id="HDF2787563.1"
                     /translation="MNLYNIIFTGSEQALGAAQAMLAEAIEKNGKEHKVAFPDTAYSL
                     PCIYAATGQKMNTLGDLEGALEVVKSLINRTHLLEHAFNAGLATALAAEVIEALKYST
                     MDAPYSEPCAGHITDPIIRSLGVPLVTGDIPGVAVVLGECPDAESAAKVIKDYQSKGL
                     LTFLVGKVIDQAIEAGVKMGLELRVIPLGYDVTSVIHVVSVAVRAALIFGGLTPGDLN
                     GLLEYTANRVPAFVNAFGPLSELVVSAGAGAIALGFPVITDQTVLEVPMNLLTQKDYD
                     KIVATSLEARGIKIKVTEIPIPVSFAAAFEGERIRKSDMFAEFGGNRTEAWELVVKKE
                     ATEVEDHKIEIIGPNIDEVDADGVLRLPLAVIVKIAGKNMQEDFEPVLERRFHYFLNY
                     IEGVMHVGQRDMAWVRISKDAFDKGFRLEHIGEVLYAKMLDEFESVVDKCEITIITDA
                     EKVSELKGEAIAKYNARDERLASLVDESVDTFYSCNLCQSFAPAHVCVVTPERLGLCG
                     AVSWLDAKATKELDPTGPCQPIEKGECLDDRTGVWNSVNETVNQISQGAVESVTLYSI
                     LEDPMTSCGCFECICGIMPEANGFVVVNREFASVTPVGMTFGELASMTGGGVQTPGFM
                     GHGRHFISSKKFAYAEGGPERIVWMPKELKDYVADKLNATVKEMTGIENFCDMVCDET
                     IADDSEGVLAFLEEKGHPALAMESVM"
     gene            complement(27491..28297)
                     /locus_tag="PC471_000423"
     CDS             complement(27491..28297)
                     /locus_tag="PC471_000423"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HDF2787564.1"
                     /translation="MEKFMIIGERIHCISPSIRKALAERDPAPILKRAKEQLEAGAHY
                     IDFNIGPAERDGEEIMTWGIKLLQSEFNNVPIALDTANKKAIEAGLKVYDRTNAKPII
                     NSADAGSRFDLIDIAAEYEAMVIGLCAKEGIPRDNDERMAYCQEILEKGLMLGMEPTD
                     ILFDPLCLVIKGMQEKQVEVLEAIKMMTEMGLLTTGGLSNVSNGCPKHVRPVLDSAFL
                     AMAMANGFSSAIMNPCDPELMKTVKSCDIINGASLYADSFLELNEGGFAF"
     gene            complement(28347..29714)
                     /locus_tag="PC471_000424"
     CDS             complement(28347..29714)
                     /locus_tag="PC471_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA decarbonylase/synthase complex
                     subunit gamma"
                     /protein_id="HDF2787565.1"
                     /translation="MALKALDIFKLTPKKNCKDCGFPTCMAFSMKVASGAVEVGKCPH
                     MSDDAIAKLSEATAPLMKALKVGAGASEYELGGETVLFRHEKTLVSRNRYAVSFCTCM
                     SDEAVDAKIANMKKVDYVRIGEQMKVEMAVLEYCGDKDAYLKLIDKIKGSGLEVAYIL
                     ACDDAQVVKEAVEVLKDARPMVYGATKENYKDMIEVVKGASLPLGVKAGSLEELYETV
                     ELIQAAGYKELVLDVTGENIKDTYTNAIQVRRTALKEQDRTFGYPSIVFANRLSNSNP
                     MMEVALSSIFTIKYGSIIVIDDISYAKALPLFALRQNIYTDPQRPMRVEPKIYPINNP
                     DENSPVLVTVDFALTYFIVAGDIERSKVPVWLVIPDAGGYSVLTSWAAGKFGGNSISA
                     FIKESKVEEVTNCKDLIIPGKVAVLKGDIEDNLPGWNVVIGPEESMELPKFLKGYQEK
                     ACQTN"
     gene            complement(29745..30689)
                     /locus_tag="PC471_000425"
     CDS             complement(29745..30689)
                     /locus_tag="PC471_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA decarbonylase/synthase complex
                     subunit delta"
                     /protein_id="HDF2787566.1"
                     /translation="MAFKMSTQKYSGKISEVEVGIGEKAIKLGGENVLPFYSFDGEVG
                     NSPKIGIQISDVYPESWTDSYKELYKDVANCPVEWAKYVEANTQADFICLKFDGSDPN
                     GLDKSVDECADVAKAVIEAIKLPLVVAGSGNHEKDGKLFEKLAQTLDGHNCLFMSAVE
                     DNYKGVGASAGMAYAHKVGAESSVDINLAKQLNVLLTQLGVKGENIVMNVGCSAVGYG
                     YEYVASTMDRIRLAAFGQNDKTLQMPIITPVAFEVGHVKEAIAPIEDEPDWGCPEERT
                     IAMEVSTAASVLVGGSNAVILRHPKSIETIKELVNALA"
     gene            complement(30709..31479)
                     /locus_tag="PC471_000426"
     CDS             complement(30709..31479)
                     /locus_tag="PC471_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDF2787567.1"
                     /translation="MGYNIAVAGKGGTGKTSLTGLLIDYLVKDKKGPVLVVDADANAN
                     INEVLGIEVEATIGEIREEVNQREKLGNAFPGGMTKAQYLQFRLNSIIEEGEGYDLLV
                     MGRSEGEGCYCFVNGILREQVNKISGHYKYLVMDNEAGMEHLSRKVTRHVDTLLLVSD
                     CSRRSIQAVARIRDLAEELKLSVGRILLIVNKVPNGVMNDGVKEEIEKHNLELIGVVP
                     MDELIYEYDSTGIPLVNLPEDSKSKVAMKEIFAKLELK"
     gene            complement(31535..32920)
                     /gene="lpdA"
                     /locus_tag="PC471_000427"
     CDS             complement(31535..32920)
                     /gene="lpdA"
                     /locus_tag="PC471_000427"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HDF2787568.1"
                     /translation="MKIVVVGGGPGGYVAAIKASMLGADVTVVEKRRVGGTCLNAGCI
                     PTKALLASSGVLNTVKEAKDFGIEIDGTIKPNFTAIMERKNKVVNQLISGIEFLFEKR
                     GVNLVNGFGKLIDKNTIEVTKDDGTVETIKADKIILANGSVPVVPRMFPYDGKVVITS
                     DEVLGLEEIPESMLIVGGGVIGCEIGQFFRALGTEVTIVEMVDQILLNEDKDVAKQLL
                     RQFKKDKIKVITGIGVQTCEVVDGKAVATLSNGKVIEAQYALVCVGRRPNLDNSGVED
                     IGIEMERGKVVVNEHLETNVEGIYAIGDIIDTPFLAHVASKEGIVAVENALGKTKVVD
                     YRAIPRCVYTEPEVAGVGKTEKQLEAEGVEYNVGQFDFRGLGKAQAIGHFQGFVKVIA
                     DKETDKIIGAAVVGPHATDLLTELSLAVHLGLTVEQVGDAIHPHPSLSEGLMEALHDV
                     HGECVHSVPKL"
     gene            complement(32964..33845)
                     /locus_tag="PC471_000428"
     CDS             complement(32964..33845)
                     /locus_tag="PC471_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HDF2787569.1"
                     /translation="MSLLRETLESGKFAVTTEMAPPKGTDLSHLIECAKPLVGRVHAA
                     NVTDFQSAVMRATSLATCKLLKDAGLEPVIQITGRDRNRIAIQGEMLSAGVFGINNLL
                     ALTGDHTSVGDHPQAKGVFDLDSVGILQTAETLMAGTDMAGNKLKGSPDFYLGASVTP
                     EYSPIEVQLLKMKKKIKAGAKFFQTQALYDINTMRKFRELTRDMDCKVLAGIVPLKSP
                     GMAKFMTANVPGIFVPDEQIERLRAAGKENWVSEGIKMAGELIKQLKEEDLCDGVHIM
                     AIGAEENIPAILDAAGL"
     gene            complement(33880..34554)
                     /locus_tag="PC471_000429"
     CDS             complement(33880..34554)
                     /locus_tag="PC471_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase C-terminal
                     domain-containing protein"
                     /protein_id="HDF2787570.1"
                     /translation="MIISENKPLEEVLGYLKDFDKLVLVGCNQCAATCKSGGEEEVLK
                     MKETLEGEGKKILGYVMLDPACNLLKSKKDLKALKEETKEADAVLSLACGDGTQTIVK
                     NLKDKPVYPANNTLFIGEVQRVGEYEEACKACGDCELGWTGGICPVTMCAKGLMNGAC
                     GGAKNGKCEVNSENDCAWIKIYERLEAIGQLDNLAEIRPPKDYSKQNNPRSLSAKKKK
                     EAAANS"
     gene            complement(34591..35463)
                     /locus_tag="PC471_000430"
     CDS             complement(34591..35463)
                     /locus_tag="PC471_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 5,10-methylene-tetrahydrofolate
                     dehydrogenase/5,10-methylene-tetrahydrofolate
                     cyclohydrolase"
                     /protein_id="HDF2787571.1"
                     /translation="MEGMSTKGQIIKGKPVADKISEELIKEVDLLIKEGINPKLTIVR
                     VGARSDDLSYERGALKRCQNIGITTEVLELAEDITQEEYIDVLKRVNDDKNVNGILCF
                     RPLPKHLNEEVIKYVIAPEKDVDCFSPINSAKVMEGDKSGFPPCTPTAVVEILKHYNV
                     DLKGSKVTVLGRSMVVGKPVSMLLLSEHATVTICHSKTKNLSGVAAEADVLIAAIGRA
                     KMVDESFVKDGAVVIDVGINVDEEGNLCGDVDTNAVLDKVSMITPVPAGVGSVTTSIL
                     AKHVVKACKLQNNK"
     gene            complement(35555..36187)
                     /locus_tag="PC471_000431"
     CDS             complement(35555..36187)
                     /locus_tag="PC471_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclodeaminase/cyclohydrolase family protein"
                     /protein_id="HDF2787572.1"
                     /translation="MKIADKTCVDFVEVLSSKEAVPGGGGAAALVGAIGMALGSMVCN
                     LTIGKKKYAEYEESVKEILSKAGKLEKDLLKMIDDDAECFLPLSKAYGLPKETEEEKR
                     IKAETMEKALKVACEVPLNIVRVCYEAIKLHEDLVDKGSRLAISDVGVGVQCLRAAIL
                     GGQLNVVININSIQDKEYVNKVKTEVDKLVEDGVKICDEVYAKVEKALGK"
     gene            complement(36292..37968)
                     /locus_tag="PC471_000432"
     CDS             complement(36292..37968)
                     /locus_tag="PC471_000432"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014355320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HDF2787573.1"
                     /translation="MGFKSDIEIAQEAKPQDIREVAKKLGLGEDDVELYGKYKAKVDY
                     NLLKRETGKKAKLILTTAINPTPAGEGKTTTTIGVADAFAKLDKNVLVALREPSLGPV
                     FGVKGGAAGGGYAQVVPMEDINLHFTGDFHAIGAANNLLAAMLDNHIHQGNALRIDPK
                     KITWRRCVDMNDRQLRNIVDGMGKKGDGAVRQDGFDITVASEIMAAFCLASDISDLKE
                     RLGNIIVGYSYEGEPVTARQLKANGAMAALLKDALKPNLVQTLEGTPSFVHGGPFANI
                     AHGCNSVIATRMAMHFADYVITEAGFGADLGAEKFLDIKCRMANLKPDAVIIVATVRA
                     LKYNGGVAKADLNNENLEALEKGLPNLLKHVENITQVYGLPAVVAINRFPLDTEAELK
                     LVEDKCKELGVNVALSEVWAKGGEGGIAVAKEVLRLLDEEENNFRFCYEDDLSIKDKI
                     NAIATKIYGADGVDYTPEADKEIANLEKLGFTKVPVCMAKTQYSLTDDQTKLGRPTGF
                     RITVRQATISAGAGFIVALTGEIMKMPGLPKVPAAEKIDVDENGVIAGLF"
     gene            complement(38217..38996)
                     /locus_tag="PC471_000433"
     CDS             complement(38217..38996)
                     /locus_tag="PC471_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDF2787574.1"
                     /translation="MKIAITGKGGVGKTTFSSMLSRMFAEDGYRVVAVDADPDANLAL
                     ALGFPKEVYESIVPISEMKKLVSDRTAASVGSFGKMFKMNPKVDDIPENFCKEYNGVR
                     LLTLGTVDSGGTGCVCPEHVLLKRLCSHLILQNKDVVVMDMEAGIEHLGRGTAQGVDA
                     FIVVVEPGERSLQTYRKVKKLGHDIGVNKVFVVGNKIRNKEDEEFIIQNLEDGESLGF
                     IYYNQDVIDSDRANQSPYDSSETTKEQIKAIKDKLMSLKDK"
     gene            complement(39306..41225)
                     /gene="cooS"
                     /locus_tag="PC471_000434"
     CDS             complement(39306..41225)
                     /gene="cooS"
                     /locus_tag="PC471_000434"
                     /EC_number="1.2.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic carbon-monoxide dehydrogenase
                     catalytic subunit"
                     /protein_id="HDF2787575.1"
                     /translation="MDEKMLTIDLNSQKMIAKAREEGVETMYDRKEGFKAQCGFGLQG
                     VCCRICGMGPCRISPKTPRGLCGADEHTIVGRNFARMVAGGTAAHSDHARDIAHTLAL
                     ADPNGNYKIRDEAKLITLAKEWDVETEGRDIYDVAHEVAEIALMEFGKPFGTLRFIKN
                     APEPRQKIWKEYAIEPRAIDREIATIMHSTHIGCTGDIDSLIHMSLRTSMADGWGGSM
                     IGTRLSDILFGTPVPRRTEANLAVLEENKVNIILHGHEPALSEMIVLASEEPDLVALA
                     KEVGADGINLAGMCCTGNEITMRHGVKIAGDFHQQELAIITGAVEAVIVDVQCIFPAL
                     ARVADCYHTKFVTTSPKAKITGSTYIEFREEQALDDAKAIVKEAILNFKNRDKSKVLI
                     PELKSGATVGYSVEAVVNQLDRVVNSHIDPAGTVKPLTDCLKSGVLRGAAGVVGCNNA
                     KGVSNEAHVTIMKELIKNDIIVVTTGCGASAAAKFGLMESDAAEKYAGKGLATVCKLV
                     GIPPVLHMGSCVDISRILDLVGAAANYLDMDMCDLPVVGIAPEWMSEKAVAIGCYVVA
                     SGIDTYLGIMPPIAGSSRAVDILTSELKDKVGATFTVNTNPKELAATIIEDIEKKRVH
                     FEALVEEKMAEKAEA"
     gene            complement(41832..42743)
                     /locus_tag="PC471_000435"
     CDS             complement(41832..42743)
                     /locus_tag="PC471_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional enoyl-CoA hydratase/phosphate
                     acetyltransferase"
                     /protein_id="HDF2787576.1"
                     /translation="MIKKLDDILEQLKGGEKIVLSVAAAHDKEVLMAIKDAVERNIIT
                     PILVGNEGKIREISKEIGFDLSGIKIVDKDDIKECAEIAVKLVSSKEADFVMKGLLDT
                     SVILKEVLNKDYGLRTDSLLSHVMVYELEKYHKLLITTDGGMNIAPDYEQKAKILKNS
                     IKAAKALGMETVKVACLAAKEKVNPKMQATVDADMLAKACKEGEFGENVIVEGPLAFD
                     LAVSKEASEIKGFKSEVSGDVDIILVPTIEVGNGIGKAFTYMADSKSAGIIMGAKAPI
                     VLVSRADSHESKLYSIAYGAIVAKNMK"
     gene            complement(42859..43437)
                     /locus_tag="PC471_000436"
     CDS             complement(42859..43437)
                     /locus_tag="PC471_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HDF2787577.1"
                     /translation="MNINEFLTHINQRKPVSSKSEIHEFMVKLSNEAMKITSKLNSSY
                     HEPEEVRSLFSELIGKKVDESFFMFPPFYTDCGKNITIGKNVFINSSCHFQDQGGIEI
                     GDNTQIGHNVVLATLNHGIAPEKRGTTYPSPITIGKNVWIGANVTVVPGVTIGDNAII
                     AAGAVVTKNVAENTIVGGVPAKLLRVIDEIPE"
     gene            43663..44586
                     /locus_tag="PC471_000437"
     CDS             43663..44586
                     /locus_tag="PC471_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDF2787578.1"
                     /translation="MEIRVLRYFLAIAREESISGAATFLHITQPTLSRQIMELEEELG
                     VKLLVRGNRKITLTNEGRLLHKRAEEIISLVDKVELEFDNPNEIISGDIYIGSGETEA
                     MRLIARTAHHFHQKYPNVRYHLFSGNADDVTEKLDKGLLDFGILIEPANIQKYDYAQI
                     PATDVWGLLMRKDSALASQSTINPKDLCNIPLIISRQTLVDKAISKWLQDSNEKLNII
                     ATYNLVYNASLLVEEGLGYALCLDRLVNTTENSNLCFRPLSPPLEAKLNIIWKKHQIF
                     SKAAEKFLLQIQKDFSSIMLDTSKLDTPKEK"
     gene            complement(44758..45900)
                     /locus_tag="PC471_000438"
     CDS             complement(44758..45900)
                     /locus_tag="PC471_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HDF2787579.1"
                     /translation="MDFKIEKYLLKKAEELAFITIKHGGEFKLKSYEVPKGGLDVPIK
                     NEVLVKGIKEKTAQDKLNSMSIADAMIYIIGIDSKFKNNAEYEKFLNALSKDIDLDLK
                     SYMGYMSRKYFEIGEHTDSLIYIKAFITMYPDDLDAMYNYAIVCQEIAKQYQKDMDDK
                     AMNAFLLEAMAKLEKVIDVDENFALGYYHLGYHYYNQGQYLKTKLTWEEALRLGLDAD
                     LVAEVQENLGKMDFKVQYEEGYTLVFQGKFKEGLEKLLPLEEEHMDWWNLLFMIALGY
                     KGMGEIEQAKMYLEKILIIKPNQVDTIVELGLCEAYKNNLDKAIEYFEQAAKIKEDPE
                     ILCNLGMAYLNNGDIDDATYYIERAYELNPQDEITIACLRELRINK"
     gene            complement(45918..47672)
                     /locus_tag="PC471_000439"
     CDS             complement(45918..47672)
                     /locus_tag="PC471_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="B12-binding domain-containing radical SAM
                     protein"
                     /protein_id="HDF2787580.1"
                     /translation="MKILLTTLNSKFIHTNLAIRYLKEFVRDLIEVNMKEYTINNDLD
                     YILKDIYKNEYDIILFSTYIWNVGDIVKLCDNLKKIRPNTKIALGGPEVSYDSYEAMK
                     KYDFVDYILYGEGELIFRDLVLHLQGKMEINDVNGLVYRQGNEIIVNKPMELLQDLDE
                     IPSPYENLNPKEYENRIVYYESSRGCPFNCQYCLSSTIPGLRYFSLDRIKSDLKALID
                     ARVSQIKFIDRTFNANRKVAMEIMDFLMKNDNGYTTYHFEVTAYLIDDKMLEFLADCK
                     EGLFQFEIGVQSTNEKTLDAVGRRDDFKKLSHVVQTVASYRNIHQHLDLIAGLPYEDY
                     KSFENSFNDVFNLGIEHLQLGFLKMIKGTGMRKVADEHGFKYKDYAPYEFLYNNYISY
                     EETLKLKDIEDILERYYNSKNFVLSMRYIIGRFYKKSPFKFFEVFAKYFDENGYFDLA
                     QGKNQLYKILMDFYNEKINIDNDVFNDILKYDYISLGKTSNIPQFFNKLDVDDFKNRC
                     HVFLQDNDNLSTYLPSFVDKPAKHIIKYVHFEPFRFNVVDLKNDINTEIREVENVVLF
                     EYDDKKIFEKSKTHKVEI"
     gene            47808..48014
                     /locus_tag="PC471_000440"
     CDS             47808..48014
                     /locus_tag="PC471_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2787581.1"
                     /translation="MELIEKVSINSLSKRDLLLIIKSLEYTNENTQISDFIDLRNNIV
                     KELCFLTDTKEKDFLDYLEKNSTL"
     gene            complement(48019..48942)
                     /locus_tag="PC471_000441"
     CDS             complement(48019..48942)
                     /locus_tag="PC471_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2787582.1"
                     /translation="MNDELLKKVYTSSFSSASRALKDLTIINTKSLIDDKTQRCVYID
                     ENRLRDELIYYRFYGEKIGNINFLNILLPLILSNTNIQKSEDEVIKLIKKYVIYFKKE
                     ELLFDYLLGSVVYNSIMHNLINNSKIEYVELLQSIKDKIIGFSIELDKSDVVKFQMAR
                     IKAIQLIDKYIDLKSEDYDEESILLNVLNVLYDVYMEDRTVENEGINSIKKSILSILG
                     EDSKLNEDNIDFIFSMSEYVVKLRKYKIGVKAYNKSIDPRSLIRLEEGNTIIDPIFNQ
                     ITVMSKTFNDNILSIKINSKSGIYILKFKKV"
     gene            complement(49196..50896)
                     /locus_tag="PC471_000442"
     CDS             complement(49196..50896)
                     /locus_tag="PC471_000442"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HDF2787583.1"
                     /translation="MQDFKVAISNCLKEKIEDLSKEEIEALIEVPPNKDMGDYAFPCF
                     KLAKVFRKAPNMIASELAESIEPSGEITKVIQLGGYVNFFVNKSQLAETVIKKVLDEK
                     ENYGHSDFGKDKTVIVEYSSPNIAKPFHIGHIRTTVIGNALYKIYDSQGYKTIRINHL
                     GDYGTQFGKLIVAFKKWGEKEVVESNPIPELLKLYVRFHDEAEQHPEMEDEARAWFNK
                     LENGDEEAQELWQWFRNESLKEFNRVYKLLDIEFDSLAGESFYSDKMNRVIELLEEKN
                     LLKESKGARIVDLEEYKMPPALITKNDGSTLYMTRDLAAAIYRKETYDFDKCIYVVGS
                     QQNLHFQQWFKVIELMGYDWAKDLIHVGFGMVALEEGTMSTRKGRVVFLEDALNQAID
                     KTKEIILAKNPNAKNVDEISKQVGVGAVVFQELSNSRIKDYTFSWERTLSFDGETGPY
                     VQYTHARCCAVLRKAEVEVTSDIDYSLLADEDSAEVLRVIESFNKNILLALKKNEPHI
                     VTRFMLDLAQAFNKFYHDNPILVENLEIRKARLALVLATKQTLENSLKLLGMHAPERM
                     "
     gene            complement(51358..51771)
                     /locus_tag="PC471_000443"
     CDS             complement(51358..51771)
                     /locus_tag="PC471_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HDF2787584.1"
                     /translation="MILVSACLIGINCKYSGDNNDNEKVKEYLKGKEFTLVCPEQLGG
                     LSTPRPPAEIVDGKVITKDNKDVTENFVRGANETLKIANLYVCSEAILKEGSPSCGCN
                     LIYDGSFSGKKIPGKGITARLLEEEGIKVKSEKDL"
     gene            complement(51839..54217)
                     /locus_tag="PC471_000444"
     CDS             complement(51839..54217)
                     /locus_tag="PC471_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HDF2787585.1"
                     /translation="MNEKSLRVLEYNKIIDLLKKKASSSLGLKYIENLVPNTDFVEVK
                     SMLEETSEAQSIIIKRGSVGLEGIHDIEDKVKRAYIGASLDPGSLIMIADTLRVARRL
                     RNSLSSSDEEDFNYPIIQSLSNSLYVYKDIEDQIYNAIISEVEISDNASSTLRDIRRR
                     IAQKNQSIRSKLNSIISSTTYQKYLQDAIISLRGDRFVVPVKSEYRSQVAGIVHDQSS
                     SGATLFIEPMTIVEMNNELRQLKLGEQEEIERILSELSAMVGEVSEDLISNQEILGRL
                     DFAFSKGKLSIQMRGIEPTLNEDKYLNIKNGRHPLLDKKKVVANTIYLGRDFHTLVIT
                     GPNTGGKTVTIKTVGLFALMTQSGLHIPADYGSSMCVYDNVFADIGDEQSIEQSLSTF
                     SSHMTNIVSILQNVTADSLVIFDELGAGTDPVEGAALAIAVLEDINSVGAKCIATTHY
                     SELKNYALTKPGVENAAVEFDIETLSPTYKLLIGVPGKSNAFEISRKLGLSDYVISRA
                     KEYINTENIALEDVLQNVEKNRIKAVEDREEAERLKEEIEKLKVEYDEKLEKLVSQRD
                     KMIEKAKSEAFSIIRQAKEEVDIIIKELRSLEQERASKEKNRKIEELRKELTSSMGSL
                     QPTVKSMIVPKVSNKEIKDLKPGEEVKVITLNQNGSVVSVDKKRKEAVVQIGIMKMTL
                     PFKSLQKTRKDVSTNVTKSTRNIIRSKSGSVKNEVDLRGLNLEEAIMEVEKYLDDAYV
                     AGLESVTVIHGIGTGVLKAGLQDILRRNRHVKSQRGGQYGEGGAGVTIVKLK"
     gene            54536..55987
                     /locus_tag="PC471_000445"
     CDS             54536..55987
                     /locus_tag="PC471_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-histidine dipeptidase"
                     /protein_id="HDF2787586.1"
                     /translation="MGNVLEGLKPESVFKNFEKISQIPRGSGNEKGISDFLLSFGKNL
                     GLETIQDESLNIIIRKPATKGYENCPGVVLQGHMDMVCEKEKNVEHDFLKDPIKLRID
                     GDMIYATGTTLGADNGIAVAMGMAILEDNTLEHPALEVLVTVNEEDGMNGADALDPSL
                     IKGQYILNMDSEEEGYLLVSCAGGKTCVVSLPVEYKEVKGDKQGLLVEVTGLLGGHSG
                     MEIVLQRANANKAIARVLSVLNVDYELASVDGGTKHNAIPREAKCVIAVNKADVESAK
                     KQINDILTAFKHEFTTSDPGMTYSVAETSVDKVLTKDCKEKVVQMSCLTPHGVQSVSL
                     DIEGLVESSTNFAIIETKESTIEFLTSVRSSVMSIRDEIADRIRLLAQALGANYDLIA
                     QYPAWEFKKGSKLEKICSETYEKLTGKVPTVMALHAGLECGLLLDKLPHAEAISIGPD
                     MFDVHTPNEHVSIPSVANVWDYVIEILKSMNQY"
     gene            complement(56191..58704)
                     /locus_tag="PC471_000446"
     CDS             complement(56191..58704)
                     /locus_tag="PC471_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HDF2787587.1"
                     /translation="MEDCVVNLKKLLLLIIVITSIFTSCIFSFGINAIGNDNRTIRVA
                     YPIQKGLTEIDEQGNYFGYTYEYLLEIAQYTGWNYEFVQVPGEINESITKLMKMLENG
                     EIDIMGGMLYNEPMQKIYNYAGNSYGVANTVLQALYENTDIDNIMSSQDVKKIRIAVI
                     ENSDTRLKELDEYCKMNLMSPELVYCKTNDEQLEALKSGKADVLLNVSMNPLEGVRTV
                     AKFSSKPFYFITPKGSTNLTQKLNSAINNIEQKDPYFSTTLYEKYFTPENNQMFLSDE
                     ETDYIKETEKLKVGVLLNKPPFQNIDKDTGELRGISIDLLNHICDKTGLEFELVPVKS
                     QEDLENMTKKREIDIVAGMIYDYEYSREHNIVMSRPFLSTQYTMMINNKLNENSLDGK
                     RVALAKGINYYGQFEENIVWYDNLEECIEAVNKGYADYTYGEGYSMQYYMNQPKYKNV
                     RLVPQTYKAKSICFGIIKKDKQELLNIFNKAIISISSEKMQSIVYQNTTYRQDFSLLY
                     IMQQNPIETIFIVASLLICIIGFMAFGLHTRTSMNEKISLELKKHTQLYEMTSEYFFE
                     YDYKQDKLIISMPEKGIETYYGLDKNDKESENGNIENERDKIRKEYNKKFFDVIKSQE
                     NGTTEMYSIMLDDTFHWIRVTSKIIYNDRKIPMYVIGKICNIDSEKEEKNRLLDKAQR
                     DSLTNIYNAAYIRKLTAQNLLNLSTEKNGALLIIDIDYFKSINDTYGHLLGDKVLIDV
                     AKVLDESFAKNDIVGRLGGDEFSVYINDIINKEELINKCNDVYNHIHLIKLPNGESLS
                     ISMGVVIAKPGQEYDELYQIADNALYDSKRQGRNRFKIV"
     gene            complement(58927..59748)
                     /locus_tag="PC471_000447"
     CDS             complement(58927..59748)
                     /locus_tag="PC471_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HDF2787588.1"
                     /translation="MIDINLEKCVGCGMCESDCLVNAIKVKDDKAKVKNILCINCGHC
                     MAICPTDAIEMQGFDKNEVIEYNRESFELEPEKLLNFIKFRRSIRQYKDIEVEEEKIK
                     NIVEAGRYTPTGGNRQPIRYILVKEKLKEVKELAIQGLYNLALDTDDNDPVRSIYKNT
                     FKKMYKRYKENGNDSLFYDAPLLMVVVGDMSLGGSAYVDGGLAASNMELMAYSQGLGI
                     CYNGFFVMASNVEPKIKELLGMSENEAVITSFILGYPDVKYKRTVNRNTAKFEVR"
     gene            complement(60266..60742)
                     /locus_tag="PC471_000448"
     CDS             complement(60266..60742)
                     /locus_tag="PC471_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
                     /protein_id="HDF2787589.1"
                     /translation="MKLTTICYIEKDDKTLMLYRNKKKDDIHEGKYVGVGGKFEQGET
                     PEECVIREVKEETGLTLKSLSYKGLITFPKFKDEEDWYMFLYFSDEFEGELSEKDLND
                     CKEGNLIWVDNDKIFDLNMWEGDRLFLNWAKTGNIFSAKIVYDNGKLKDYNVSFLD"
     gene            complement(60776..61354)
                     /locus_tag="PC471_000449"
     CDS             complement(60776..61354)
                     /locus_tag="PC471_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HDF2787590.1"
                     /translation="MREEALEILFKYLETDRMRKHCYAVEAVMRELAKRLEPEKEEEW
                     AVAGLLHDLDSDIVGRKPGEICEGHATTTVELLKKENFGDEEMYRAILGHHDGMGVTR
                     TSLMEKAIYAADPITGFITAIALVYPDKKLTSVKTKSVIKRMKETRFASNVNRDAMRS
                     IEDIGIPFDEFAELSLNAMKNISDVLESAENN"
     gene            complement(61458..64040)
                     /locus_tag="PC471_000450"
     CDS             complement(61458..64040)
                     /locus_tag="PC471_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HDF2787591.1"
                     /translation="MKKIELLAPVGSFDSLKAAVQNGANAVYLGGKDFSARASANNFD
                     RNELIEAVKYSHIRGVQVFVTVNTLIKQYEIEDFVEYVKFLYDIDIDALIVQDIGMAR
                     LIKKLLPDFELHASTQMVAHSLEDVKYLQSVGFDRVVLARELNVDEIKWICENTTVDI
                     EVFVHGALCVCYSGQCLMSSMIGNRSGNRGRCAQPCRQKYELIDINTGEVVKSNGDYL
                     LSPRDLSTIEDLDKIIEAGVLSLKIEGRMKKPEYVATVVSGYREAINEFVDKKKISIS
                     EEIINNLYTIFNRKFTKGYILGEVGKDIMNSNRPNNQGLYIGKVLDYNRKNKRLRVML
                     ENTLKKGDGINLGGGTIGRIIKDGEITTIAHKGDLIELDFVGEAKRNQLIYKTSDSEL
                     MDRVQATFQQDKEFIKTNIRAKVSIKLGKKPELFISDFCGNKSVVEGDKIVEKAIKVA
                     LSKEKIETQIQKLGNTPYKLKEIEIDLDEGVSMPISVLNQMRRDCIELLDEERIKIKD
                     RKFKKNRLKYQPNRVNKQKKLDTVPKIRVKVKNLEQLEAVLNYNVDLIYYEDINTLSK
                     ALQMVSSISIDSSISMDLYTSSSLGIPKELMGSNTNNIYTLSNTSKIDTLNKNHSNTA
                     VTDKKLIYSAPRIVRNKEYKQFEILDNIKGINKVQIGNWGSMEYFKKKDLNIDCYLNA
                     FNSESINHFKEEGASTVCLSQELNLNEIKQTLKYTDVEIEAVIYGYAPMMVTEYCPMG
                     VVVRDCKKDKRVAKCKESSYALRDFTDASYRITQDIFCRSTIYNSKVTCMLDNLYELE
                     EIGIDVFRIDFTVEDSEMVKKVIEAHIEVLSNNYKLGKRATDLYKKLDEIGTVAGHYY
                     KGVE"
BASE COUNT        19950 a        12017 c         7085 g        25133 t
ORIGIN      
        1 acactaaata atatctttca cactgttgct ttttctttaa taatactaaa tagtatcttt
       61 cacactgttg ccctttcttt aataatacta aataatatct ttcacattgt tgctttttct
      121 taaattacac taaataatat ctttcacact gttgcttttt ctttaataat actaaatagt
      181 atctttcaca ctgtttctct ttctttaata atactaaata atatctttca cattgttgct
      241 ttttcttaaa ttacactaaa taatatcttt cacattgttg cttttcttta ataatactaa
      301 ataatatttt ccacattgtt gctttttcta aacaactata aataatatat taaaccctta
      361 tttctttttc taataagtta aataatacat tttacacata tctatttgtc ttgcacataa
      421 taaataatac attatatttc tatctaaatt tagattctaa attaaatagt ataaattata
      481 ttcttcaata aacaagtttt caaatccaag attcgctctc tatctttaaa ctttattgta
      541 aacataattc catctttttt aattatttct ccttcatgaa attttttatg gtatacctta
      601 tcccctacac caatactgtc aatttctttt ttcgttggag attttatatc attaataaat
      661 aatgatttat caactttctt accatactta tttaaagtag agcttataca taacttttct
      721 tcagctcttg taatagcaac atacataagt cttctttctt cttctaattg ttcttctttt
      781 ttctcttcct tacaaatatc atatgatttt tcatgaggta ttgttccttc atttacacct
      841 ataatataaa catttctaaa ttctaatcct tttgcactat gcatagttgt aaatatgaca
      901 ccctcagtct gcttattctt gttattttcc gttaactcag tctttactct atcaatatgc
      961 tctaaaaact ctgtaacagt attaaaatta gttgctgaac tctcaagttc atttaatatc
     1021 tctacaagtc cattagtctt tatctttctg ttgctacaat aatctaaaac atatctatca
     1081 taatcaagac tagttctaat ataagatatt gcatttttag gatttaaagt atttaaataa
     1141 cttaaatcta tttccaaatc atttattgtt ttaacttgtt taggatgaag attacactta
     1201 tttattaatg ctgttataaa gtctttttca tccttaacca tatttatact gtctttggat
     1261 atatatctaa aaggcttgtt tataattctc aaccaatctt tatttgattt tggattaata
     1321 cctattctta aataggctag tatgtcttga cttgcccaat ggtcatatat tgtaattact
     1381 gagtctttta ctacaaatgg aatcctcata tccataaata catctactaa tgctcttgac
     1441 tgtatattag ttctataaat tactgcaaaa tctgaatact ctacataatc tttttgtatt
     1501 tcgtcaatta tttccctggc aatatataag gcttcttcct ctgaatcatg cggagaaata
     1561 taggtaacac ttcctccatc cccctgacta cacttaatga ttttttcata tctattttta
     1621 tttttgtcga taagtctatt tgcagtatga actatctcac ttttagacct gtaatttata
     1681 tctaatatta tttttttagt attattaaag tattgttcaa attcaagcaa aaaatctggt
     1741 ctagcacctc taaatccata tatactttgg tcttcatctc caactgcaaa tatattgttt
     1801 aatgggctac aaattaactt taaaacttca aactgtactt tatttatatc ttgaaattca
     1861 tctatcaata tatatttata cacatttcta accatttcta gtgcagactt attgttcaaa
     1921 agtaaataat aagttcttat aagcatatca tcaaaatcta ttttattaac tttagatttt
     1981 tgttcttcgt ataagttgta tacttttaaa aactcatctt ttgttaatac ttctgaatta
     2041 aattcatttt tatccataag ttcatttttt acataggaaa tttcatttat aacttgtcct
     2101 atattctcat catcatcagc attctctata tttaaacttt ttaggattgc cttgattgtc
     2161 attcgctttg acttttcatc aaatatactg tctaagttgt atctttcaaa gtatcttaga
     2221 attttaaaaa atacagagtg aaaagtacca taggttacct tattaagtct tatgtcatca
     2281 cttaaattca aagctctatt tttcatttct attgaagatg ccttggtaaa acttattgcc
     2341 aagattcttg ttggtgctat gtttttgttt acaaccatat tggctattct atatgtaatt
     2401 actctggtct tacctgaacc aggccctgca agtatcatgc aaggcccatc tatatgctca
     2461 actgcttcta attgattttc atttaacttt cttatatcta tatttgttgt gattattagc
     2521 tccaccacct tgatttataa ttatattatt ttctttttaa ttataaacta aaagttcata
     2581 aaaataaatt taatttttcc tctattaaac cacatcattc ctattttcat gacatatctt
     2641 gagttctttt ttaattttgt cgcttatatc ttcaggtata aatgatggtt tactaatata
     2701 gtagccttgc atataatcta ctccaagatt aattagtgtt tccatttcac cttttgtttc
     2761 tacaccttct gctatcactt taatatttct ttcgttagaa tatgagataa gattttttaa
     2821 tattttctgt ctgttttcat ctttatcaat acctcttaca atagacatat caatctttac
     2881 atagtctggc attatatata atagtacagc atctccgttg taacctgtcc caaagtcatc
     2941 taaagcaatt tctatattcc aatggtctat atatgctttc tttttctcaa taaaatcttc
     3001 attaatctta tcattctctg ttatttctag tactactcta tttaaatatt caccatataa
     3061 atcttcaatt tcctcaaaat ctacttttga caaattatta ttaggaatag aatttaagaa
     3121 cagtttgcaa tcgccaaatt tttctttgta ttttttaaat gatttaagtg ctttaaacca
     3181 tgtaatacgc tcaatttgat ataatttaga ctgtgaacga gctaatctaa tgacatctat
     3241 tggcgaacta aaagttttta ttttaggtct cataagtgct tcatatgcaa aaactttacc
     3301 tttacaatct acaataggct gaaatgcata ctcaactaat tgaccatcaa ttaacttgtt
     3361 taattcttct ctattactca ataagaatga atccttatta tatatattaa tatcaaattc
     3421 actaacttca cccttgattg tatttttaat ttgatacata gcaaaatctg cgtatctcat
     3481 taattcatca tagttggttg agtcatcagg gtaccaggaa attcctgctg atgcccttat
     3541 tttaaattct gttccatcat gaagttttaa taaggtatta ttcatctgtt tcttaacaga
     3601 attaataata tctcttatac tatctttact ttcatatcca tatataaata cataaaactc
     3661 atcacctgaa agtctagata ctataccatt ccatgatata aacttcttaa gtatgtctgc
     3721 agcacaccta atatattcat caccagcatc atgtccatat gtatcattaa tatattttag
     3781 attatctaaa tcccacataa taaatgctgc tatcttaagt tcatctttat tcttaaattt
     3841 ttcttgtatc tgtgaatgaa aagctctcct atttaaaaga ttcgttaaaa tatcaaaatc
     3901 tctttcatat tctatcttcc ttttctcaat aaattctttt gttacatcaa caacaacacc
     3961 aagaaccttt agctcatctt caaagatatt cattcttatc cacttaagtt ttttatcttt
     4021 agattcaaac ttatatatat atgtattttc tgtttcataa ctttcttgca aatatttatc
     4081 tagtcccctt aattttttca ggaacttatt tttttccata tatccatatt cttcaaattt
     4141 gatttcagta gtaaacacgc taaaaaattc ttttgtacaa aaaacattct ctgcgttttt
     4201 gtataactca aaagctccaa ttggcatatc taccatttca ataatctgtg ataattttga
     4261 cgaagaatct gcaacatttg cactgagcaa ctctatagct gaagataatt catctatttc
     4321 agatatattt atcttatcaa gatttactgg ttttgatgga ttactgtttc taactttgtt
     4381 ggctaaaaga acaattggtt ttgtaaataa tcttgatgca atataaatac ctattagacc
     4441 tattaataaa gaaattgcta tagatattat aactaatttc tctacccttt ttgaaaatga
     4501 caaaagactt ttttcttcaa taatacccaa aagagcccaa cgttcatttt caaatggagt
     4561 attggagtta tataaatcta agtattgtac actagcataa atgttgccat taccacattc
     4621 attatcttta tttttaatct tatatatatt tttatacgat tccttctgct ctaaattaga
     4681 atgtaaacca tcccaaaatt tttcttttaa tgagtatcca tttgaaatta cattttcaaa
     4741 ggccatatca ttatttctat ctacacctaa aatataatta ccctttttat tcccatttat
     4801 ctctgagtat ggaagtaatt ctcttaaata atttacactt aaatctattc caagtactcc
     4861 atatacagtt ccatcttcat aaataagggg aactgtatat gtcataagcc tatcacctgc
     4921 atttgtcaat aaaaacttac ttccccagta tcctaaatta gaacttgata catttggatt
     4981 atcaattgca gctctaaatg gtttataaaa aaaatcatct ccatcttgct cattttcact
     5041 tttaaatgaa aacttaggtt cccaataact gtccatagat attccaaaac ttcttgctat
     5101 atctgatgaa cctctctcaa ttaacaaatc agaattatca tttggattaa acttagggtc
     5161 taaatccctt atatacaatc ctgtcctact tatttcacct ttactatcta ttgacttatc
     5221 gttaatattt ttgtcactta ggattataaa agctcctgta acagaatttt ttctcaaaag
     5281 atatattaaa tcatttgata tgctttttat aatatctttg ttaatttctt cattgttgcc
     5341 tacatcctca attaaggcat ttttttcatt taatactttt tttacagagg aattaattgt
     5401 agcttccgtt tcacttgtat ttgaccatct ttgtaacatt tcattttgta tataattttt
     5461 acgattaatt actctttcat ttaagatgtc aaacgaatta tcatttaatt tcttaatagt
     5521 tcctccccat aaaattgtgg cacaaaataa acttgtttga attagcataa caataattag
     5581 gggaaatatc atctttttca ttattgaatt gttttttctt agattgtttt ccatacccat
     5641 tccacctata cctttgtgct cgtcaacaca ctaactgcca attcatctat ttttttatag
     5701 cattttcaag ctcagcctta aaactttcaa accaagtttt aaaattttta tcattattaa
     5761 attgtgctac tgcatccttc ttagaagttc cactttttac taattcattt attttatttg
     5821 aatcttcttt agctttatca agcattgaat attcaagtat ttgtctagct ttagttccac
     5881 catcaaatgc tttattagta tataaattac aactgttaat ctgttcaata gatgaggcta
     5941 ttgaaccttc aactttatca gacaaactca aattatcttt cttcataact tcttttataa
     6001 catccaaatc attcgttttt ttctttactg gtaagtatcc agaagcaata gagaatttta
     6061 tattaggttc tttatctgta aaccatttta aaaattctac tgatgcatat tcttcttctt
     6121 tagtagattt tgttacagcc attcctgcac cttgttgtac agcatatttt ccttctcctt
     6181 taaaacgtgg tgcttgaaaa acagatgttt caatattata actctcatta tcatttagca
     6241 ttactctatc tggaaagtat gctgctcctg aactagaacc tacaagagct aatatatcac
     6301 ctgtttttgc atcatcagaa cgaaacttac cataagaagc aaaatatcca tttatatatg
     6361 gtacatagaa attatcccat agttttttca taactttttc atcaatattt agcgtaactt
     6421 taccatcttt aacagaaaaa atttctactc ctagttgttt acttcctata ataatataat
     6481 ttgctaaagc atctcttcca aaaaatgctt taccatcatt aggctctttc gttaaactat
     6541 ctgtccattc ataatatttt tttgatgtct ctactaatcc ttcaattgtt tttatatcat
     6601 ttatatttga atttgtagct tttgcaaatt tatcccaatc agttttattt aacatcaaaa
     6661 tttcagttga ttttgcaatt gggaaaagtt taaactcatc cttagaattt attctgcctt
     6721 cttctacata ttcgtctatg tattcattta attcttcttt acttatgtac ttatccatat
     6781 ctactagtaa ccctaattta tccatttcat atgcagtatc tgagtatgct gcaaatatat
     6841 ttggtacatc ttcagaacca acttttttat ttgcagaatc taaaactttc tctgataaat
     6901 caactacatt accttgactg aatgcttcaa caataattcc tttttcagca cctacagtgt
     6961 cattaaattc cttaacaagc ttatcaaaag ccattttctg ctgtccatta taataatgcc
     7021 aaatttgtat tgatgtcgga ttttctgggt ctaacttata attatcttcc ttctttaaac
     7081 tacatcctgt aagtaataaa acaatcattg atgtgataat taattttgca aacttacacc
     7141 tattcataaa atctactcct tcaaattata aaatttataa tagcattaaa actgtaactt
     7201 cactatttat attaattata gaacgtttat tgatatattt aaacaggatt tacaacatat
     7261 ttaaaagcaa ttaagaaaca tactattaca aatatagaaa ataaagcttt tacataattt
     7321 ttatttctaa atgcaccaaa tgacaccaat ccttgttgaa tagctactgt aattactaaa
     7381 agtatcatta ttatattaag ttgaaccagt ttaaataagt aaagcaatga aaacactact
     7441 agaagtacac ataatacaat actagattta ttaataatat catcaatctt atctactttc
     7501 ttagattgct tattaatttt atttaatttt tctttattta aattctcaac ttttttatta
     7561 tacctttctc ttgttttatc tttatattta gtatttttat ttttatttcc catttatttt
     7621 ccatcctcta taaatttttt aattgagctt atacattatt taaatattat atatttaaga
     7681 taccatatat tcctacaaaa aggtaactta ctatgtatct atttttgatt ttcgcataaa
     7741 aaagccatct cctttagaat taattctgta ttgagacagc taatttaact atttatcctt
     7801 tgaaacattt tctacattat catatttact atcaactaaa actatatcaa ctcttctatt
     7861 ttttgaccta ccttcttcag atgaattttc tgctattggt ctaaactcac catatcctac
     7921 agcagataat ttatctggtg ctatacctgc attgtcaata agaagttgaa caacattggt
     7981 tgctctcata actgataaat cccaatttga cttaaactct gaatttttta tagacatatt
     8041 gtctgtatgg ccttctactc tcatgtaact attcatttca tttaacatct tacctatttg
     8101 aataatctta tctctagaat tatccttcac aatagctttc cctgtatcaa atagtattgt
     8161 atctttaagg cttattacta atcctctatc ttgaactctt aatgatactg aattagccat
     8221 accattttcg tttaaatact tctgaatttc ttcaccaatt tttttaaact tagcattttg
     8281 taaagtatct aaatcattac ttcctggttc aactactggt tctatcttgc tattaccacc
     8341 ttcaataact ccagaagaac caccaaaagc agaatttaga gattgagata attgtttata
     8401 tttctccgca tcaacattac tcatagaata cattattaca aagaaaatca taagtaatgt
     8461 tatgaggtct gaataagtta aaagccatcg ctcattgttt tcttcttttt catcttcatc
     8521 atacaacata aacattccat ccttttatat tattcagctc taccaccaat ttcttcaagt
     8581 tcaattagtt cttttgaatt taaaaatatt tttaattttt ctgtcattaa attagaatta
     8641 tcaccctcaa gtatggataa tattgcttct atcaccaaaa gtctttcact tacttcttgt
     8701 tgatttatag ccttaagttt tgatgctatt ggtaaccata ttaaattggc agaaccaaca
     8761 ccatacaaag tagccaagaa tgcaacagaa atttgaggtc ctaatgcatc tggattactt
     8821 gaaagattac ctaaaatatg tactaatccc ataactgtac ctattatccc cagtgtaggt
     8881 gcatatccac ctgctgaatc aaaaattgct attccttctt tatgtcgttt tgatgttatc
     8941 tgtatctgtg attccaatat atttcttata gtaactgcat ccactccatc taccataagt
     9001 tcaagccctt ttctaataaa agggtctaat tcatcattct cacttatttc tttgtcaata
     9061 ctaagaagcc cctctttcct agcctttgtt gcaatttctt tgaaatagat tatattttct
     9121 atgttattag gctttttatt tttaaaagct aataacatta tctttataga ctttttaaac
     9181 acagggaatg gagtcgaagc tccaacagct cctatagttc caccaaacac aacaagtgct
     9241 gatgaaaata caaataatgc tccaacatgt cccccatcaa taacaaaagc aactaccaaa
     9301 gatgctatcc ccactatgaa aaaaattaaa gttgtcatct attccctcct ttctttagtt
     9361 atacatatct aatcatttat gacattataa aaagactatc tttaataaaa atagccttta
     9421 ataattggcg gagaaggagg gattcgaacc ctcgcaccgg ttacccagcc taatccctta
     9481 gcaggggatc ctcttgagcc acttgagtac ttctccatat ctctatacta ataaattata
     9541 taataaaatc atattttagt caattgtttt ataaaatttg agttattttg aattattaaa
     9601 catctatttt tacttttttg cttaaatgta agttttaaat atggtaaaat tatcttgcat
     9661 aacacttata atattatttg ataaatatac atacattaac aaatatacct aaactctgac
     9721 ttaattgtaa gttatgccaa aactaaaata atactagtcc aaggaggata ttttatgtta
     9781 ccacaggaaa gatatgagaa gattttaagt attctcaaat cagataatat aattaaaata
     9841 aatgacatag ttcaaatgtt tgatatatca atagaaactg cacgaagaga tttagcccat
     9901 atggagagta tcggtctttt aaaaagagtt tatggtggtg ctataccagt tatagcaagt
     9961 agcgtagaac ttaactatgt taaaagaaaa aaaataaact caaacgataa aaaacagatt
    10021 gctattaaat gtagtgagtt tataaataca aatgatacta tttttatgga tactggaact
    10081 actgtacttg aagtttcaaa aaatttgaaa gataaaaaga atctaactgt aataaccaac
    10141 tcaattttag ttgcaaacga acttatgaat actaatataa aagtatatgt gcttgggggc
    10201 tatcttagag aaactgaagg ttctttttct ggtccgctta cactgtcagc tattgaacaa
    10261 tttaatgttg ataaagctat tttaggcgct ggaggtatca ctcttgaaaa tggaatctct
    10321 gactatcact tagaagaagc acttgttaga aaaaaaatga ttgaaaggtc tagaaaatct
    10381 atagtagttg tcgaacctcc taagtttggt ataaattcat ttgcatcagt agctcctata
    10441 gaacatatag atactattat tacctctgct agtatagacc ataatataat aatagagttt
    10501 tctcaaaaag gaataccttt agtcatagct gataattagc ttatatgatt gatttttagt
    10561 tccttgatgt aataaattaa aattaaggag ccaaataatt atttatttag ctccatttat
    10621 gtctaaacta tactttatat tttaattatg taactacttt cagttagctt atcattttta
    10681 gttatttgat tgccatttta tttaatttat aagttttagt tattcgatta ttttttaatt
    10741 gtctgtcttt ttaatttatt tttagttatt taaactccta ctttaaaata atttaaattt
    10801 gacttaagta ttgtttattc tacactgcat ctttcttcat tttaatactt tgttcttctt
    10861 ccaaggttaa atcttttgtc ataagtatag caagtgcaat tgccaatact cctccaacca
    10921 attttccaat tacaacaggt gtaatcatct ctggaactac tcctgctgta aatcctaaat
    10981 ggtcccccaa agctgctgta gcacaaacca gccaagcagt atttactact ttcccctttg
    11041 gactcatatc tttcatcatt ttatatactg gaatactatt ggctaaagta aaaactaatc
    11101 ctgctgcact agtggcatcc atacctaatt ttccacccac tattgtaagt ggtttattaa
    11161 gaactttaac taataaatgt aatattggaa aagtacctaa taacacgatt gcaatacttc
    11221 caattacttg aataccttct cttataggtg tcatgccttt tataagtact atacctgtta
    11281 tttcttgaaa tgctgctgct cccaaaccta tcgttatcaa tatagatata aattttccaa
    11341 aaattaatga cccatttatc ataagagtag aattaaattt taaacctact gccaatatta
    11401 ctgccaatat tatcactggt atcatattta taattaccat tgataagtta aatcctgcta
    11461 ttaatcctcc tataatacct ccaaagggta ttgttatcaa gcctatcagt aacccttttg
    11521 caaagaatgg tctgtcttca tattctataa gacctagtcc tactggaatt gaaaatacta
    11581 atgtacatcc aagcatggat gacacaacca atcctgcaag cattcctgcc tctttattgt
    11641 gtgcaagttc catagccaat ggataccctc ccatatcatt cgctaaaata gttgcaaaca
    11701 tagatgggtc agctccacag agattgaaga ttggtattat tataggacct agtacatctg
    11761 aaattactgg agctaaacaa actataccaa ccattcctaa agccaatgga cccattgaat
    11821 taaacccttc ttcaaactgc tcgccaagtc caaatttatt tccaattatt ctatctaacg
    11881 ctcctaaaag cactcccaca gccattatgt ataatataat tttatcaaag ggcattttta
    11941 attcctccta tacatgtaaa atactctatt gtaacttaat tagcacacac ctttttggta
    12001 tcgttttccc aattcatagc tctttttact gcttggttcc atccaagtaa tatttcttct
    12061 cttttttgag tagtcatatt tggaatatat tttttgctta ttgtaaattc acttctaatt
    12121 tcatcaatac tgttccagac accacaagaa agacctgcca tatatcctac tccaagtact
    12181 gtcgtctcag gattttctgc aatttcaatt ggtataccta atatgtcgga ttgaaattgc
    12241 attgtaaagt tatttttagt aattccacca tctactctca acatttttat ttttttccct
    12301 gaggctaagt tcatagaatc aacaacatct ttgacttgaa gtgcaacaga ctcaagagtc
    12361 gctcttacta tatgctcttt tttagtactt cctgttatcc caaccattgt acctctagca
    12421 tattggtccc aataaggtgc tgctatacct gcaaatgctg gtacaaaata tacacctcca
    12481 gtacttgata tggaatttgc catgtcatca gtttctgaat aattttttat aactccaatg
    12541 ccatctctaa gccattgtat tgcagcacct gctatataca ctccaccatc ataagcatat
    12601 gtaactttat ctcctatttt ccaagcaaca gttgtcaaaa taccattacc tgctatattt
    12661 agttcctcac ctatattcat atataggaaa catcctgtac cataagtatt tttaaccatt
    12721 cctggttcaa aacatccatt gccaaataaa gctgctggtt ggtctaccat tgaagcagta
    12781 atttttatag aaaccccaca aaatgcctcc ttatctgtag ttccaaaatc accactcgta
    12841 ggcattatat ctgctaaaat acttctaggt actcctataa tgtcaagcaa ctctctatcc
    12901 caatccatat ctcttatatt aaaaagcatt gttcttgctg ctgtagatac atcagttaaa
    12961 aaagattttc ctcctgtcat tttccatatt aaccaactat ctaatgttcc tgcaagtact
    13021 tctccatttt taattttttc ttttacacct tttacattat ctattaccca tttaattttt
    13081 gtacctgaga aatatgggtc tataattaac ccagtcttat ctattatctt attttctaaa
    13141 cctggtatat tctttaaatt ttccacataa tcataagttc tcctgcactg ccacacaata
    13201 gcattgtaaa gtggtttacc tgtattttta tcccataaca ttactgtttc accttgatta
    13261 tctataccaa ttccttttat atcactttca ttgattccat tttcaaaagc ctgttgtaat
    13321 acattattac tcacagtttt tgtattattc caaatttcta tagggtcatg ttcaacatat
    13381 ccagattgtg gatatatttg agtatgttcc atataatcgc taaaaacaaa ctctgatttt
    13441 tcgtttatca atactgctct tgttcctgtt gttccttggt caattgctaa tacatatttc
    13501 actttaaatg tcctccttta ttttcagaat taataaattc ttttttattt tttgaatata
    13561 taatctattc aagttattta tattttatat catcatgtgg atttatgcaa tattcattac
    13621 aatatttttt gttattttta attatagtga ttgaaaaagt atttttattg aaatattgtt
    13681 gcaatattta ttattttatt ctataatcta gttaaaataa cacataatat tttaatattt
    13741 acacataatc agtcattcaa aaatttaaat ttaacttttt aaggaggctt tacaatgaaa
    13801 aaaacatatc tattcagtcc tggaccagta atggttacag ataaagtcag aaattcactt
    13861 cttcactatg atatatgcca tagaggaaat gagtttatgg atttattcaa agatacacaa
    13921 caaaaaatca ataaactata taatgcaact tctaattact actcattaat tgtatctggc
    13981 tctggaacat ctgtaaatga atgtgtacta tcttctatct ttgacaaaga agatgcagtt
    14041 ctgctagtta gtaatggtgt ttttggagaa aggctagaag aaatattatc tacttataat
    14101 gtaaaaacat ataaacctaa ttttgaatgg gcagaatacc cggacctaga cattttagaa
    14161 aaatacataa ttgaaaactc tgatataaaa gtaattgcta tggtgttcca tgaaactagt
    14221 tcaggaatga taaatccagt ccctgaacta ggaaaacttg ctaagaagta taacaaaata
    14281 ttttttgtag atgcaataag tgcctcagca ggtgaatata ttgatgtgga agagtttaat
    14341 atagatataa taacaggtgt tggtggcaaa gctcttggag catttccagg ttctgcatac
    14401 ctttgtgcta aagagagtat tttacaaaat attaaagaaa atcaatgtaa gaatgtgtat
    14461 cttaatcttt ataaacacta taaagttgct aaatcatctt ctcaaacacc aaatacacct
    14521 aatgttactt taatatttgc ccttaatgaa gctttaactg agtttttgga agatgacagt
    14581 aaaatagaaa gatacaaaga atgttctgct attttaaggt atggtatgga agaattgggt
    14641 ctaacatttt tacttccaga taagtatatg tctaatacag taacatctgt atttctacct
    14701 aaagaaatca atgtaaatga gtttatatta gggctagaaa gggaaagtgg ctatgttgtc
    14761 tatccaggaa aaggcaaatt cctagatgct aacatattcc aagtagcaaa tatgggagaa
    14821 atatatccag aagattgtta taaattctta gatatcttaa aatcaaaatt ggagataaaa
    14881 aaagttatat aacagtttgt ttgtaaaatc ctacaatata tcagtataaa ttaaatagac
    14941 tgattttttt aaattttcct ataataaatt atattttata tttttgtata agggtgaact
    15001 ataatatgta aagttcaccc ctatttcaaa tctttttcct tcaatataat ctttttcttt
    15061 taatttattt tatatttttg acgcaatcgt tttttattca atcattttat attcttgata
    15121 caatcattct tacacttaat tattttttgt ttttgacaca atcgtttttt gttgaatcat
    15181 tttatattct tgatacaatc attcttacac ttaattattt tttgtttttg acacaatcgt
    15241 tttttattca gtcatttaat attttcaaca caatcattct tacattctat tattgtttgt
    15301 ttttgataca actgttttta ttcagtcatt taatattctc aacacaatta ccttttcatt
    15361 caatcaaagt aatctttaag cattattttt caagctcatc aaagtaatta tatacgataa
    15421 cctcacctgt ctctttatct gtagtcttta taatgccctt agctccattt acctcttctt
    15481 ttgtaaattc atcatctaac acaactttga atttatcttc ctttccataa ccttgttgaa
    15541 attcatctct caaaattgca tttttttctg cttttggctt caactcaaat tcaattccag
    15601 atttcatttc atcaaaataa tttaatacta caacctcatt attcatttta ttagttgtct
    15661 ttataatccc tttagctcca tttacttctt cttttgtaaa ttcatcatcc aatgttatat
    15721 taaatttgtc ttcttttcca tattcctctt gaaaatccct cttcatttca ttgtatttag
    15781 tttcaaactc tttactcttg tttaattcca tagctgatac atttgtcaat gatgctccaa
    15841 ttgccattac tgttaatgcc attactatac cacttttaaa tttttttact ttcatatctt
    15901 acctcctaaa ttaaatgttc tcaatgaaat tatataaaga aaatcctaag ttttacagtt
    15961 tcattcttgt aactaaaatt actattatgt aaccaaactt tttgcttcaa ctattcctta
    16021 aaagttgctt ttttgaggaa tagttgaagt taaataatta gattttatct atatttatag
    16081 atttaaaatc aaatttatcc atataaataa atacttattt ttgcaaaata aaaattttta
    16141 attactccaa tccttcttat ttacttaaaa tcctatgata agaaatttaa aagtctttac
    16201 cttctttaac aataatttat ttacttttaa catacaaaat atagagagta tatcaaaact
    16261 attttactga atattctaaa aataagtacc ccctattagt atatatattt acatattaaa
    16321 aaaaagtttt aaataaagtt atatttttta caacagtttt gtaattaaaa acatattgaa
    16381 ggtaaattta aaggtactat atactataaa tatgtcgaca caataaattt aaaaaggaga
    16441 actaaatatg aaaaaaagat taataataat gatattgtct gttgtcttag tactatcaag
    16501 tatacttacg atatttgcat attcaaatat taaatataat aataacaaat tgatatattc
    16561 taatatgata gataaaaaaa ctcaaaatag tgtaaaggaa atcctaaaag aaaataaaat
    16621 taatgaaaaa gacatagata catttattaa atcagtcaac aattataata aattgcaagt
    16681 taaaatactt caaaataata taaatatatc taaatctgga tattcttcaa tcaatgcaaa
    16741 acaagttcca tataacctag aaaaattgca agataattgg gtgaaaaaat tccctgatta
    16801 tatggatgta aattgtagaa taactgcttt tagacttttt aaagatttta taaattcaaa
    16861 taagaaattt actggagact ctattgattt gaatgtcgac ttggacacta taatgaataa
    16921 taaagatgct aaatttagca ccaaagatgt agaaaaattt atcaacttct tttctgcaat
    16981 accagctaaa gatacagatg atacaatcaa aattgcagaa caaataaaaa atgaatggaa
    17041 aaaaagaaaa atatctttta aaaataataa aaatatatct attataaatg gatttcttcg
    17101 ttatccagaa actaaaaatg tatttatagg tcatacaggc atatgtatta aaactaaaaa
    17161 tggcattcta tttttagaaa aatacggtgt tacatcacct tatcaagtta ctaaatttaa
    17221 aaataaagaa gatgtaaaaa attatatgtt taatagattg aagatgtcag aaggtgaaat
    17281 tgaattacct gaccctataa taatggaaaa cgataaactt atgaaataat aactttaaac
    17341 gcaaaatagt atttataatt actaagtcaa tataatagag aaggtctata gatttgtgta
    17401 aatacaagct tatctacaat cttatataag aatgtttata taaataagat agattaattc
    17461 taagtcctat tattctattg aattttatta ttattttatt tagattctat tgttatttta
    17521 cttagaactt attattattt tgtttaagtt ctattactat tttcaatcat ataaatttac
    17581 aaaagactgt acataaattt attttataca aacttatcta cagtcttttg tgtgaatata
    17641 tctaaataat atcgtattta tttatttcct ctgcacagtg agaatcagaa cctaaaacta
    17701 atggaatatc gtattttttt accaagtcta ataagtatcc atctatataa ggctctttgc
    17761 agtactcttt tctatttcca gaaacattat aatcaagttc ataacctttt tctttaatta
    17821 actttattaa ctcttcaaga attttatttc ctttataatc gtatggaaat acctgattaa
    17881 actttctaac taaatttaaa tgacctattc tttttggttt atgtatacct aaatctgaat
    17941 ttacagcttt tatcaatgtt tcataatact tattataaac agcttcaata cttcctaatt
    18001 tgtcgattaa attttgaaac tcctctacac tgtaatctac acaataataa tcatcatcaa
    18061 tcttaattat atgtactgat aaaagagaat cctctaattc ctttccatat ttatttaaat
    18121 tctcctttat cccttcttcg tatccttcta taaaatccac ttccgaccca acatttatct
    18181 tgattttacc ctcatacttt tctttcaact tcttgacatc attaaaatac tttggtaaat
    18241 cttcaatact catacaacta tcattttcag gagcagggtc tctaaatcca tttgcatacg
    18301 gaaaatgttc tgtaaatgtc atttctgtta ttccaacatc cattgctctt tgtacatatt
    18361 tttcaaaatc atccttactt ccatgaggac aataaggtga atgtatatga ccatctttca
    18421 taaataattc actccttttt taaagaaaaa atctaatata aatgcataaa tcttttaact
    18481 tcaaaataat atttttaatc atgctaattt ttattatatc taatattaga gtaattttaa
    18541 actgcctttt aagtatctta tcattagtac tattttcaac ttaacattaa tattattttt
    18601 taacttaaca ttaatactat ctttaaatag taatactcaa tatataatta cttaacataa
    18661 aataactcaa attgcttgat gaggcttttg cagtatttaa agaatatata attatttaac
    18721 attaaataat ccaatcttgt aataaaattt actcattaaa gtatattaca gaaaatacct
    18781 caaaataaac tttttagttc atgatatact cttttttgca tgaaattaga tatattttta
    18841 tcatttcaac aatgaaaggg aggcttatat ccatctttag acaacccctt agataaaatt
    18901 tttaataatt aaacatcaca aaatataaaa atcgcttata tcaaacgtat aagcgattaa
    18961 ttatatttac atatgttttt tagcctctaa caacatttta ttagcttctt ccatatactc
    19021 tatagccttt tcaatactct ttgctgtttc ttcttttcca atagctctag ccttttcaac
    19081 ccactctcta aatccttctt catgagtttc attgtgattt atccaatgta ctaaaagtat
    19141 ttttaaagtt ttttcatcct tagactctga tgattcctca tgactatgtt cgtggtcgtg
    19201 gtcgtgagca tggtcatgtg gatgttcatg acatatatct ccatgagtat gttcgtggca
    19261 gtggtcatgg tcatgatgtc catgatgata tcttacaatc tctttatcaa aaatcattag
    19321 ttattccccc tgttatttta tattaaactc tttttttata aggtttacag aatcttttac
    19381 aagtagtgct aatggtttac cttctaatcc aactaactcc acattttctg gcataatagg
    19441 taaaagtatc ttaagtgcct cactgtctgc aatagcttca cttatattac aagtaacttc
    19501 tcccatcata gaattaggta ttattacaga aataggtgca actattatat ttgctttttt
    19561 agctgatact actatagcat tttcccctgt agctccttta ttagcatgag ctttcatcat
    19621 agaactagtt gctatagaat tagtccctag tgcataaatt tctacatatg taggtaactc
    19681 ttctctcaaa gaagaaacta tctgggctcc aatacctcca cccattccat ctatcactgc
    19741 tattatcata tgaaattccc ctattaatta attttcttgt attattatct tatggtccat
    19801 caatcttatt tctttaatta tcccatctat tatcttttgc tctcctaata aatccgttaa
    19861 ataaatcttt ccatcacaag ggtctatatt aaccacattt tccataacct tttctaactc
    19921 attattactt ttacaaatga aagctgatga ttcacacata taaatctcct cctaatatga
    19981 tttttattaa aaatcaaaaa aagattccct taggtatttc aatacaccaa agaaaccttc
    20041 atgatttcag tttttgtata ttattatacc ttaagtatat cactttaatt tatgtaaagt
    20101 caattaaagt taacgctttt tattatactc ttcattcttg tatttttcat atagttcttt
    20161 aactaattgc ttctcataat cagtcaaatc gccctccaca aactcaacat taaattgttc
    20221 agctaatccc tttactatgt ttttctgtag aatatctatg tctatttttc tgccaatttc
    20281 attttctatt ccacttgctt ttttagctgt aaatttcata gctctttctt tcaattctgg
    20341 tgtcttagtt tttattaatt taaacaattt ttctacatca aaatccagta ttattgaacc
    20401 atgttgtaat aaaacaccat cttttctact ttgagcactt ccaattacct tcttattatt
    20461 aatagtaact tcatatgatg catgtgcatt aaaacatgca gctgataaat tttccctact
    20521 tattctctcg cctctattta aattatctgt ttctatacca cttagattca accctttaac
    20581 tagaccttca cttatatatc tataagatag gttgatgctc ttatcgatta aaggattatc
    20641 ttctcctata attatactat aggttagctc attatcatgt aatacagctc ttccacctgt
    20701 aattcttcta gtatagtcta tattcataca tctacactta tctaaatcta tctcatcttc
    20761 caatttctga aaatatccta tactcaaaca agctggttcc catgtataaa atctaagagt
    20821 tggcttatta tgtcctttct tatatgcagt aaatatagct tcgtcaattg ccatattcat
    20881 tgctccttca tatgatttat tatgtataac tctccactga ttcatgatta ttacgcctcc
    20941 atttttagta taaatatatc ctagaaatcc tctatagatt tatttctctt attgctaaat
    21001 ctatctcact ttgatttaaa ggtcttgaat agttgtatat agtacttcct ggtctttcat
    21061 tatagtatgg tctattacaa tccttacaac ctcttatttc aaaaggatag cctttgttta
    21121 tgtcttttaa aatatcattg tcaagcttta tactatttaa gtatccattt ttaaacttaa
    21181 aatactcttt tggataacct ttatttatca tataagacat taactgtacc cttctataac
    21241 tttctatgct tggttgattt atcttttcca ttttagttcc tctaactgga gtaaatgcaa
    21301 ataaactaat cgttacatca ttttccttta aataagtata taaattgtat atatcttcat
    21361 gactttcacc cataccaact attatatgtg tacttatttt attgggatat gattttgaca
    21421 tttcagttat aaattttaac ttttcatcat agttatttcc ttttattttt tcatatagtt
    21481 ctttatttgc tgcatctatt gctattccta ttttatcaac tcctgctgaa aaaaacttct
    21541 ttatctgttc atcattttct aattttgcag atagtgaaat tggcatgtct atcttaccac
    21601 ttatatagtt tataaagtct aaaacagagt tatgtgcttc ttcacttgcc attgattgaa
    21661 tacatattct ctttatattt tttcctttat aagcttttaa agcatctaat atttcttctt
    21721 ttgaaaactc tggccaaatt actcttgata atttatcttt tctagtacta ctttctattg
    21781 attgagaaca aaaactgcat ttatttatgc atttttctcc aatcattata tatgctgtgg
    21841 taggaggtat atcgcttttt ttgtttaata tcccaagttc tattgctgtc ccaactgata
    21901 gtctaatcat aaaacgcaac actccttaca ctcatttatt gtcatattca tttctatagc
    21961 cttattcttt gcagaccttg atggtaatac tattctattg actccacaca ttatagataa
    22021 ttggtctagc tcttttctat acactcctct tggtctcata caacctagat taatttcagt
    22081 atctggtaaa ttaattctag cttcacataa aatatctgct accccttcta actcaggagg
    22141 ttctacattt tcatactcag tcccttttgt tggaataagg actataaatg taagcttatt
    22201 aggaggattt ttttgtaggt attctataat ttcatattca ccctttattt gtcctccttt
    22261 taaaccaata catatatgtg gtgctacttc tgtatgttct tgtattgtat tatagacttc
    22321 aatataatct tctttactct ttttcatctt ataaacttct cttattgtct catcatcaaa
    22381 cactaaatca aaagaaacta tatctaaata cttacacaac tctacaatgc tatctttatc
    22441 cataagacct aaatgagcat tcaatctata tccttgctct tttaagtttt taatggtatt
    22501 aatatgagta tttataggga catctccctc ataactacaa ccgccactca atagaaagct
    22561 agttatattc cttgattgta ctttttcctc atattcttct attggcacca tattgtttaa
    22621 gtagtgaccg ttgcaatggg cacatttcaa actacattga ttgcttgtag tactaagagc
    22681 tagtgtctgg ttaggataag caaactctat tttatttcca aaattcttta atttaatatc
    22741 aaatgctatt ttcatttttt cagatagatt cttattcata gtttattcct tcttaagtac
    22801 attttactct tctagtaatt tctttgctgt tgggaactgc tctatattcg tatgtggtaa
    22861 gaaacatgca gaaataaatt catccatata acttggatat acactaagtt caacataagt
    22921 tatttgatta cctatttctt caagtttatt ttttgcatct ttacttataa gcgctaagta
    22981 agaaccaaca agtgaactat tccctatata agtaaatttt tctctatcaa tatcaggaag
    23041 taaccctata attattgagt tttctatgtc taggttgttt cctataccac ctgctatata
    23101 aaccctatct aatatactaa aatccatacc taaactatct acaagagtgt atgcgcctga
    23161 atatatagct cctttagctc tgatgaaatt atctatatca acttcattga ctactatatc
    23221 attttctaaa tcaaattctt ctttaaatgc taatacatac tctcctatct catgctcatt
    23281 aaatctaact ctcttattat ctaaatccct gtatatttta cctcttctat ctattacacc
    23341 tttagtaatc atttgacaga ttaaatcaat tataccagaa ccacatatac caactggtgc
    23401 acattcatca ataatcttta atgttggctc taaagtatct ttatcaataa ttactttctc
    23461 tattgctcct gctgatgctc tcattccaca gcttatccct ccaccttcaa aagcaggtcc
    23521 tgctgaacat gcacagctca tcatataatc tttatttcca aatacaatct ctccattagt
    23581 tcctaaatct ataaataaaa cattttcttc acttgaccat atccctgctg ataatacacc
    23641 tgctgttata tctcctccta cataacttgc aacagatgga gctaagtaca catatgcact
    23701 gtcatttaca aaaagaccaa cattttcacc cataagtttt ggagatttta aaaatggtgg
    23761 tatgtaaggt tcttgtctta agaagtctgt atacacaccc aagaataaac ttgtcatagt
    23821 tgtatttccg gcagccacta atgtaactac atcttcctta tctattccat ttctttcata
    23881 tattgttttt aatagtggat ttatagtttc ctcaactata gctttatgaa gtgtctctaa
    23941 tccatttttc ttagttgaat atataattct atttataaca tctgctccat attttatctg
    24001 tgcatttcca gaagatgctt tatctactac ctcttttgag tataggtcta ctaaacaaac
    24061 aactactgaa gttgtaccaa tatctattgc aactccgtat aaggaatttt ctttatttcc
    24121 ttgttctata tttatgattg taagtttctt ttgtttttga acatatgtta ttgtaacctt
    24181 aaaatcactc tttctaaata cagttggcat ttttctaagt atatccaatc taaaatctat
    24241 ttcattatat cctaggttat ttctaacata tctttccaat ctatctacat cacttatatt
    24301 atcatctaaa tttggttctt ccatttctat gtatttcttt tttatatttg ttttaaattg
    24361 aaggttatgt tcttcaatta tttttttagc tctttcaaaa atttccctat cctctttttt
    24421 attactacct tctatcttca taccatgcat agaagatgat actttagaag gaacttcaat
    24481 ttctatatct gatattacct ttgtacaaca agctagtata taaccttgct cccactcatc
    24541 atctgttata tgtcttgttt tttctgtatc aacttttcca ttcaataatt ttactttaca
    24601 cttcccacaa gacacgtttc cattacaagg agcatctatg aatatatctg catttcttgc
    24661 aacctctagt aatatatccc cctcattaca ataaacctct ttattgtttg gtgtaaaact
    24721 tacttttatc atgttatccc tcccaatttg ttctatatat gataaaagga aagtatagct
    24781 aaatttgcgt gcactcaaat tttactatac tttccccaat tataataata tcttaatctt
    24841 taattattct aatccagcct cgtattcttt atcacttaat aacgtatcaa gttcagccac
    24901 atcagctagt tttactttta atatccagtt ttcatatggg tcttcattta tacattctgg
    24961 ctcatcttct aatttttcat taacttccaa tacctcacca ttaacaggag atattaaatc
    25021 agaagctact ttagaagact caactactcc aaaatctact cccttagtta cagtatcttc
    25081 tacttctggt gtttcaacaa acaatatttc tcctaattga tcttgagcat agtctgttat
    25141 acctatatat acaacatctc catcaattac ttttacccac tcatgatctt tagagtattt
    25201 taattctggt aataatttca tttcgataga cccccaatat tatattattg attaaattac
    25261 ttaaatcata ttaatattat atcatattat tttaaatttt ccatcaatta cattacactt
    25321 tccatagcta atgctggatg acctttttct tctaagaaag ctaatactcc ttcagaatcg
    25381 tcagctatag tttcatcaca aaccatatca cagaagtttt ctattccagt catttcttta
    25441 actgtagcat ttaatttatc agctacataa tcttttaatt cttttggcat ccaaactatt
    25501 ctttctggtc caccttctgc ataagcgaat tttttagaag atataaaatg tcttccgtgt
    25561 cccataaatc ctggagtttg aactcctcca cctgtcatag aagcaagttc tccgaaagtc
    25621 ataccaactg gagtaacact tgcaaattct ctgttaacta caacaaatcc atttgcttct
    25681 ggcattatac cacagataca ttcgaagcat ccacaagaag tcattgggtc ttctagtata
    25741 gagtataatg taacagattc aactgcacct tgagatattt ggtttacagt ttcatttact
    25801 gaattccata cacctgttct atcatctaag cactcgcctt tttctattgg ttgacaagga
    25861 cctgttgggt ctaattcttt agtagcctta gcatctaacc agctaactgc tccacaaaga
    25921 ccaagtcttt caggagtaac aacacaaacg tgagctggtg caaatgattg acataagtta
    25981 caagaataga aagtatcaac actttcatca actaatgaag ctaatctttc atctctagca
    26041 ttgtacttag ctattgcttc tccttttaat tcagaaactt tttcagcatc tgtaattata
    26101 gttatttcac acttatcaac tactgattca aattcatcta acatcttagc atataaaact
    26161 tctcctatat gctcaagtct aaatccttta tcaaatgcat ctttagagat tcttacccaa
    26221 gccatatctc tttgtccaac gtgcattaca ccttctatat agtttaagaa gtagtggaaa
    26281 cgtctttcaa gaactggctc gaaatcttct tgcatatttt taccagctat tttaactatt
    26341 acagcaagtg gtaatcttaa tacaccatct gcatcaactt catctatatt tggccctatt
    26401 atttctatct tatggtcttc aacttctgtt gcttctttct taacaacaag ctcccaagct
    26461 tcagttctgt ttccaccaaa ctcagcaaac atatcgctct ttctaattct ctcaccttca
    26521 aatgctgctg caaatgaaac tgggataggt atttcagtaa cttttatttt tattcctcta
    26581 gcttctaatg aagtagctac tatcttatcg taatcttttt gagttaatag gttcattgga
    26641 acttcaagaa ctgtttggtc agttataact gggaatccta aagctattgc tccagcacct
    26701 gcagatacaa ctaattcact taatggtcca aatgcattaa caaatgcagg aactctatta
    26761 gctgtgtact ctaataatcc gtttaaatca ccaggagtta atccaccgaa tattaatgct
    26821 gctcttactg caacagatac aacgtgtata acagaagtta catcatatcc tagaggtata
    26881 actcttagct caagacccat ttttactcca gcttctatag cttggtctat aactttacca
    26941 actaagaaag ttagtaaacc tttagattgg taatctttta taacttttgc tgctgattct
    27001 gcatctggac actcaccaag aacaactgca acaccaggta tatctcctgt aactaatggt
    27061 acaccaagtg atctgattat agggtcagtt atatgacctg cacatggctc actatatgga
    27121 gcatccatag ttgaatattt taatgcttca attacttctg ctgctaaagc tgtagctaaa
    27181 ccagcattaa aagcatgttc tagtaaatga gttctattta ttaaagattt tactacttct
    27241 aaagcacctt ctaagtcccc taaagtgttc atcttttgtc ctgttgcagc atatatacaa
    27301 ggtaatgaat atgctgtatc agggaaagca actttatgct cttttccatt cttttctata
    27361 gcttcagcta acatagcttg agccgcacct aaagcttgtt ctgaccctgt aaagattata
    27421 ttatatagat tcattctatt tcccccttta atatttacac ctatttttta ttaactatac
    27481 cctaaaaacc ctagaaagca aatccacctt catttaactc taagaaagag tctgcatata
    27541 aagatgcccc gttgattatg tcacaagatt ttacagtttt cattaattct gggtcacatg
    27601 gattcattat agctgaacta aatccattag ccattgccat tgctaagaat gcactatcta
    27661 aaacaggtct aacatgctta ggacatccat tagatacgtt agataatcct cctgtagtta
    27721 aaagtcccat ctcagtcatc atttttatag cttctaaaac ttctacttgt ttttcttgca
    27781 taccttttat aactaagcat aatgggtcaa atagtatatc agttggctcc attcctaaca
    27841 ttaagccttt ttctaatatt tcttggcagt aagccatacg ctcatcatta tctcttggga
    27901 taccttcttt tgcacataaa cctataacca ttgcttcgta ttcagctgct atatctatta
    27961 aatcaaatct tgagccagca tcagcagagt ttattattgg ttttgcatta gttctatcat
    28021 aaactttaag tccagcttcg atagcttttt tattagctgt atctaatgct ataggaacat
    28081 tgttgaactc agattgaagt aatttgattc cccatgtcat tatttcttcg ccatctcttt
    28141 cggcaggtcc tatattaaaa tctatatagt gtgctcctgc ttctaattgc tcttttgctc
    28201 tttttaagat tggagctggg tctctttctg ctaatgcttt tcttattgaa ggtgatatac
    28261 aatgtattct ctcacctata atcataaatt tttccattta taatgtcccc ctttaaattt
    28321 aatattaata gttagttaat attttattag tttgtttggc atgctttttc ttggtatcct
    28381 tttaagaact taggtaattc catagattcc tctggtccta taactacatt ccatcctggt
    28441 aagttatctt ctatatcacc tttaagtact gcaacttttc ctggaatgat aaggtcttta
    28501 caattagtta cttcttctac cttactttct ttaatgaaag cagagattga gtttccacca
    28561 aatttaccag cagcccaaga tgtaagaact gaatatccac ctgcatctgg tattactaac
    28621 catactggaa cttttgatct ttctatatca ccagcaacta tgaagtaagt taatgcaaag
    28681 tcaacagtaa ctaatactgg agaattttca tctggattgt tgattggata aatctttggt
    28741 tcaactctca ttggtctttg tgggtcagtg tatatgtttt gtcttagtgc aaataatggt
    28801 aatgccttag cataactaat atcatctatt actattatag aaccgtattt tatagtgaat
    28861 attgatgata aagcaacttc catcataggg tttgaattag ataatctatt tgcaaatact
    28921 attgaaggat atccaaatgt tctatcttgc tcttttaaag ctgttcttct aacttgtata
    28981 gcattagtgt aagtatcttt tatgttttct cctgttacat ctaatactaa ttctttatat
    29041 ccagcggctt gaattaattc aactgtttca tataattctt ctaagctacc agcttttaca
    29101 cctaatggta aacttgctcc tttaactact tctatcatat ctttgtagtt ctctttagtt
    29161 gctccatata ccattggtct agcatctttt aatacttcaa cagcttcttt aactacttgt
    29221 gcatcatcac aagctaaaat ataagctact tctaatccac tgccttttat tttatctatt
    29281 aattttaaat acgcatcttt atctccacaa tactctaaaa cagccatttc aactttcatt
    29341 tgttcaccaa ttcttacata gtcaactttt ttcatgttag ctattttagc atctactgct
    29401 tcatcactca tacaagtaca gaatgaaact gcatatctat ttctacttac taatgttttt
    29461 tcatgtctaa ataatactgt ttctccacct agttcatact cagatgcacc agcaccaact
    29521 tttaaagctt tcattaaagg tgcagtagcc tctgataatt ttgctatagc gtcatcagac
    29581 atatgtgggc atttacctac ttctacagct cctgaagcaa ccttcataga gaaagccata
    29641 caagttggga atccacaatc cttacagttt ttctttggtg ttaatttaaa tatatctaaa
    29701 gcttttaatg ccattatttt ttccccctaa ccttaacaat ttaattaagc taatgcgtta
    29761 accaattctt ttatagtttc tattgatttt ggatgacgaa gtataactgc gtttgaacca
    29821 cctactaaaa cacttgctgc agttgaaact tccattgcta tagttctttc ttctggacaa
    29881 ccccaatctg gctcatcttc tattggagct atagcttctt taacatgacc tacttcaaaa
    29941 gctactggtg ttatgatagg catttgtaat gtcttatcat tttgaccaaa tgctgcaagt
    30001 ctaattctat ccatagtaga tgcaacgtac tcatatccat aaccaactgc tgaacatcca
    30061 acattcataa ctatattttc acctttaacg cctagttgag ttaacaatac gtttaattgt
    30121 ttagctaggt ttatatcaac agaagattca gctcctactt tatgtgcata agccatacca
    30181 gctgatgctc ctactccttt gtagttatct tctactgctg acataaataa gcagttatgt
    30241 ccatccaatg tttgagctaa tttttcaaat aatttaccat ctttttcatg atttcctgaa
    30301 ccagctacaa ctaaaggtaa ttttattgct tcaattactg ccttagctac atcagcacat
    30361 tcatcaactg acttatctaa tccatttggg tcagaaccat caaattttaa acaaataaaa
    30421 tctgcttgtg tattagcttc aacatattta gcccactcta ctggacaatt agctacatct
    30481 ttgtataatt ccttatatga atcagtccag ctttcaggat aaacgtctga tatttgtata
    30541 cctatttttg gagaattacc tacttctcca tcaaaactat aaaaaggtaa tacattttcc
    30601 ccacctaatt taattgcttt ttcccctatt cctacttcaa cttctgatat ttttccagaa
    30661 tatttttgag tagacatttt aaatgccata tttttttccc ccttaagcct attttaattc
    30721 taatttagca aaaatttctt tcatagccac tttagatttt gaatcttcag gtaaattaac
    30781 taaaggaata cctgtagaat catattcata tatcaattca tccataggta caacaccgat
    30841 tagctctagg ttatgctttt caatttcttc tttaacacca tcattcataa ccccatttgg
    30901 aactttattt acaatcaaaa gtattcttcc aacacttaac tttagttcct cagctaaatc
    30961 tcttattcta gcaacagctt gtatgctacg tcttgagcaa tcactaacca ataaaagtgt
    31021 atcaacatgt ctagtgactt ttctacttag atgttccata ccagcttcgt tgtccatgac
    31081 taagtatttg taatgaccag atattttgtt tacttgttct ctaagtatcc catttacaaa
    31141 acagtaacat ccttcacctt ctgacctacc cattactaat aagtcatatc cttcgccttc
    31201 ttcgattata gaatttaatc taaattgaag atattgtgcc tttgtcatac caccagggaa
    31261 tgcattgcct aacttttctc tttgatttac ttcttctctt atttctccta ttgttgcttc
    31321 aacttcgata cctaacactt catttatgtt agcattggca tcagcatcta caactaatac
    31381 tggtcccttt ttgtccttaa ccaaataatc aatcaaaagc cctgtgagac ttgttttacc
    31441 agttccacct tttcctgcaa cagctatatt gtatcccatt ttaagacttc ccccttaaat
    31501 ctagccacag agccttatgt actctgtctg tctattataa tttaggtaca gaatgaacac
    31561 attctccatg tacatcatga agtgcttcca ttagcccttc agataaactt ggatgtggat
    31621 gtattgcatc ccctacttgt tctactgtta atcctagatg aacagcaagt gacaactctg
    31681 tcaataaatc tgtagcatga ggtcctacta ctgctgcacc tattatctta tcagtttctt
    31741 tgtcagctat aacttttaca aatccttgga aatgtcctat agcttgtgct tttccaagac
    31801 ctctaaaatc aaattgacct acattgtatt ctacaccttc agcttcaagt tgcttttcag
    31861 tcttaccaac accagcaact tcaggttcag tataaacaca tcttggaatt gctctataat
    31921 ctactacctt agtcttacct aaagcatttt caactgctac gataccttct ttagaagcaa
    31981 catgtgctag gaaaggagta tctattatat cgcctattgc gtatatacct tctacattag
    32041 tttcaagatg ttcattaact actacttttc ctctttccat ttcaattcct atatcttcta
    32101 ctccagaatt gtcaaggtta ggtcttctac caacacatac taatgcatat tgtgcttcta
    32161 taacctttcc attagaaaga gttgcaacag ccttaccatc aactacttca caagtttgta
    32221 ctccaatacc tgtaataacc ttaattttat ctttcttaaa ttgtctaagt agttgtttag
    32281 ctacatcttt gtcttcattt aatagtattt ggtcaaccat ctcaactata gttacttctg
    32341 ttcctaaggc tctaaagaac tgccctatct cacatcctat aactccacca cctactatta
    32401 acatagactc aggtatctct tcaagtccaa gaacttcatc actagttatt acaacttttc
    32461 catcatatgg gaacattctt ggtacaactg gaacagaacc atttgctagt attatcttat
    32521 ctgcttttat agtttctact gttccatcat ctttagttac ttctatagtg tttttatcaa
    32581 ttaattttcc aaaaccatta actaagttta ctcctctttt ttcaaataag aactctatac
    32641 cacttattaa ttggtttaca actttatttt ttctttccat tatggcagtg aagtttggct
    32701 taattgtacc atctatttct ataccaaaat cctttgcttc ttttacagta ttcaatactc
    32761 ctgaagaagc aagaagtgct ttagttggta tacatcctgc atttaagcaa gttcctccaa
    32821 ctcttctttt ttcaacaact gttacatctg caccaagcat agaagctttt atagctgcta
    32881 catatcctcc tggtccacca cctactacta ctatcttcat ctttaattcc cccaaaccta
    32941 atataatcta caatcttgca atactataaa cctgcggcat ccagtatagc aggtatattc
    33001 tcttctgccc caattgccat tatatgaaca ccatcacata aatcttcttc ttttaattgt
    33061 ttgataagtt cccctgccat ttttattcct tcacttaccc agttttcttt tccagctgct
    33121 cttaatcttt cgatttgttc atctggaaca aatattcctg gtacatttgc agtcataaat
    33181 ttagccattc ctggtgattt taaaggtact attcctgcta ataccttgca atccatatcc
    33241 cttgttaatt ctctaaactt tctcattgta tttatgtcat aaagtgcttg tgtttggaaa
    33301 aatttagccc ctgcttttat tttctttttc atttttaata attgaacttc tattggagaa
    33361 tattctggag ttactgatgc tcctagataa aaatctggag aaccttttaa cttattacca
    33421 gccatgtccg tacctgccat taaagtctca gcagtttgaa gtatccccac agaatctaaa
    33481 tcaaacactc ccttagcttg tggatggtct cctacgctag tatggtctcc tgttagtgcc
    33541 aacagattgt ttatcccaaa tacacctgct gacaacattt ctccttgtat agctattcta
    33601 tttctatctc tccctgttat ttgaataact ggctctaatc cagcatcttt taataattta
    33661 catgtagcaa gagaagttgc tctcataact gccgattgaa agtctgttac atttgctgca
    33721 tgaactcttc caactaacgg ctttgcacat tctattaaat gtgaaaggtc agtccctttt
    33781 ggaggagcca tttcagtagt aactgcaaat tttccacttt ctaatgtttc tcttaataag
    33841 ctcatctcta atcccccttt taaccttcta aaaaaacttt tatgaatttg ctgctgcttc
    33901 tttctttttc tttgcactta aacttcttgg attattttgc ttagaatagt cttttggtgg
    33961 tcttatttct gctaagttat ctaattgtcc tatagcttct agtctttcat atatctttat
    34021 ccaagcacaa tcattctctg agtttacttc acacttacca tttttagctc caccacaagc
    34081 cccattcata agacctttag cacacatagt tactggacat atcccacctg tccaaccaag
    34141 ttcacagtct ccacaagcct tacaggcttc ttcatattct ccaactcttt gtacttcccc
    34201 tataaacaaa gtattgttgg ctggataaac tggcttatct tttaaattct taactattgt
    34261 ctgtgtacca tctccacatg ctaaagataa aactgcatca gcttccttag tctcttcttt
    34321 tagagccttt aaatcctttt tagactttaa aaggttacaa gcagggtcaa gcattacata
    34381 tcctaagatt tttttgcctt caccttcaag agtttccttc atttttaaaa cttcttcttc
    34441 cccaccactt ttacaagttg ctgcacattg attacaacca actaaaacca gcttgtcaaa
    34501 atcttttaag taccctagca cttcttctaa tggtttattt tcagaaatta tcatatctac
    34561 ttccccccta aaccttccac atatatatat ctatttattg ttttgtaatt tgcaagcctt
    34621 aactacatgt tttgcaagta tagatgttgt aactgaacca acccccgctg gaactggagt
    34681 tatcattgat actttgtcta aaactgcatt tgtatcaaca tctccacata aatttccttc
    34741 ttcatcaaca tttataccaa cgtctataac taccgctcca tctttaacaa aactttcatc
    34801 aaccatctta gctctaccaa tagctgctat tagcacgtct gcctctgctg ctacacctga
    34861 tagattttta gtttttgaat gacatattgt aacagttgcg tgttcactta ataaaagcat
    34921 tgatactggc tttccaacta ccattgatct tccaagaact gttaccttac ttccttttaa
    34981 atcaacatta tagtgtttta atatctcaac tactgctgta ggtgtacatg gtggaaatcc
    35041 acttttatcc ccttccatta ccttagcaga gtttattgga ctaaaacaat ctacgtcttt
    35101 ttccggtgca ataacatatt ttataacttc ttcatttaag tgttttggaa gaggtctaaa
    35161 acataatata ccattaacat ttttgtcatc attaactctc tttaatacat caatatattc
    35221 ctcttgtgtt atatcctcag ctaactctaa cacttctgtt gttataccta tgttttgaca
    35281 tctctttagt gctcctcttt cataagataa atcatcactt cttgccccaa ctctaactat
    35341 tgttagttta ggatttatac cttctttaat tagtaaatcg acttctttta ttaattcttc
    35401 actaatctta tcagctacag gttttccttt tataatttgt cctttagttg acattccttc
    35461 catacattca caactcccct acaagcaaac ttaaattaat ttattctata taccacatat
    35521 taataacata gtatctactt tcgtcattta aaaattattt tccaagagct ttttcaactt
    35581 tggcataaac ttcatcacaa atttttacac catcttcaac taacttatct acttctgttt
    35641 ttaccttatt aacatattct ttatcttgta tagaatttat atttataact acatttaatt
    35701 gtccaccaag tatagcagct cttaaacact gaactcctac accaacatca cttatagcaa
    35761 gtcttgaacc tttatcaact aaatcttcat gtaacttaat agcttcatag caaactctta
    35821 ctatgtttaa tggtacttca caagcaactt ttaatgcctt ttccattgtt tctgctttta
    35881 ttcttttttc ttcttctgtt tctttaggaa gcccgtaagc ttttgataat ggtaagaaac
    35941 attctgcatc atcatctatc atttttaata aatctttttc taactttcct gccttactta
    36001 atatctcttt tacactttct tcatactcag catacttctt cttccctatt gtaaggttac
    36061 ataccatact acctaaagcc atacctattg cacctactag tgcagcagct cccccaccac
    36121 ctggtactgc ttctttggaa gatagtactt ctacaaaatc aacacaagtt ttatctgcta
    36181 ttttcatgat aatcctcctt gaaaggatgt aggaaaatat ctcaaattgt tcgagatatt
    36241 ttacgaacta ttttaagata tctcctactc tttatttata aattaatttc cttagaataa
    36301 tccagctatt actccatttt catcaacatc tattttttct gctgctggaa cttttggtaa
    36361 tcctggcatc ttcattattt ctccagtaag tgcaactata aatccagctc ctgctgaaat
    36421 tgttgcttgt cttacagtta ttctaaatcc tgttggtcta cctaatttag tttggtcatc
    36481 agttaaagaa tattgagtct tagccataca tactggaact ttagtaaatc caagtttttc
    36541 taagttagct atttccttat cagcttcagg agtataatct acaccatctg ctccataaat
    36601 cttagtagct atagcattta tcttgtcttt tatagataaa tcatcttcat agcagaatct
    36661 aaagttattt tcttcttcat ccaataatct taaaacttct ttagctactg caattccacc
    36721 ttctccacct tttgcccata cttcagaaag agcaacatta acacctaatt ccttacactt
    36781 gtcttcaaca agtttaagtt cagcttcagt atcaagtggg aatctgttga ttgcaacaac
    36841 tgctggtaat ccatatactt gagttatgtt ttcaacatgt tttaataagt ttggaagtcc
    36901 tttttcaaga gcttctaggt tttcgttatt taagtctgct ttagcaactc caccattgta
    36961 ttttaatgct cttacagtag caactattat aacagcatct ggttttaagt ttgccattct
    37021 acattttata tctaagaatt tttctgctcc aaggtcagca ccgaaaccag cctcagttat
    37081 tacataatct gcaaagtgca tagccattct tgtagctata acagagttac atccatgagc
    37141 tatatttgcg aatggtccac cgtgtacaaa tgatggtgtt ccttcaagag tttgaactag
    37201 gtttggtttt aaagcatctt ttaataatgc agccatagca ccattagctt ttaattgtct
    37261 agctgttaca ggttcacctt cgtagctgta tccaactatt atgtttccta atctttcttt
    37321 taaatcagat atatcacttg ccaaacagaa cgctgccatt atttcagaag caacagttat
    37381 atcgaatcca tcttgtctaa ctgctccatc acctttttta cccataccat caacgatatt
    37441 tctaagttgt ctatcgttca tgtctacaca tcttctccaa gttattttct tagggtctat
    37501 tctaagtgca tttccttggt gaatatggtt atcaagcata gcagctaaaa gattgttagc
    37561 agctcctata gcgtgaaaat ctcctgtgaa gtgtaagttt atatcttcca taggaacaac
    37621 ttgtgcatat cctccaccag ctgctccacc tttaacacca aatactggtc ctagagatgg
    37681 ttctcttaaa gcaactaata catttttgtc tagtttagca aatgcatcag caacacctat
    37741 agtagtagta gtttttcctt cacctgctgg agttgggttt atagctgtag ttagtattaa
    37801 cttagctttt ttaccagtct ctctttttag tagattgtag tcaacttttg ctttgtattt
    37861 accataaagt tctacatcat cttcacctag tcctaatttt tttgcaacct ctctaatatc
    37921 ttgaggctta gcttcttgag ctatttcaat gtcagattta aatcccataa ctgaatctcc
    37981 tcccacaacc taaatagatt taaaatataa tttttgtaaa aattgtatta atatgtaaaa
    38041 attatatttt ataagataat tatacatgta aaattactta aaaactagct actaagctat
    38101 taaaatattc aaacttaata gctagttatt tatctaatta cttacttatt acctatctat
    38161 tgcctatgtc taacctttaa ccaacttaaa atcccaaatc aaaatttcta tctattttat
    38221 ttatccttaa gtgacattaa tttatctttt attgctttaa tctgttcttt cgttgtttca
    38281 ctagaatcat atggagattg attagctcta tcagaatcaa taacatcttg gttataataa
    38341 ataaatccta aagattctcc atcttctaag ttttggatta taaattcttc atcttcctta
    38401 tttctaattt tatttcctac tacaaatact ttatttacac ctatatcatg tccaagtttc
    38461 ttaacttttc tataagtctg tagacttctc tctcctggtt ctacaactac tataaaagca
    38521 tctacacctt gggctgttcc tcttcctaag tgttcaattc ccgcttccat atccataacc
    38581 actacatctt tattttgtag tattaaatgt gagcagagtc tcttcaaaag aacatgctct
    38641 ggacatacac atccagtacc accagaatct acggttccca atgtaagtaa tcttactcca
    38701 ttgtattctt tacagaaatt ttcagggata tcgtctactt taggattcat cttaaacatt
    38761 tttccaaatg aacctacaga agcagctgtt ctatctgaaa ctaatttttt catttctgat
    38821 ataggtacta ttgattcata tacttctttt ggaaaaccta gtgctaaagc taaattagca
    38881 tcagggtcgg catcaacagc aacaactctg tatccgtctt cagcaaacat cctagagagc
    38941 attgaagaaa aagtcgtttt acctactcca cctttacctg ttattgctat cttcataatc
    39001 cctaccacct tttattacta tttgcttttt agtagttaat tttacagtta aatatttttt
    39061 atactctata tttttataca ctaaaaataa actacagcta gtccttacag gaaccaatta
    39121 aatttaccct actagctttt caaagcagca tgtatagttt tattattctt taatattgtt
    39181 tatattttat ttattcagta tattcattat attttgatgt tctttacttt aatatttgac
    39241 tttattcaac tttattcaac cagaaacttc ttataaaaac gtttattcta taacttcact
    39301 attttttaag cttcagcctt ttctgccatc ttttcttcaa ctaatgcttc aaagtgaaca
    39361 cgttttttct ctatatcttc tattattgta gctgctaact cttttggatt tgtatttaca
    39421 gtaaatgtag ctccaacttt atcttttaat tcactagtta atatatccac tgctcttgaa
    39481 gaaccagcta ttggaggcat tattcctaaa taagtgtcta taccagaagc tacaacatag
    39541 catcctatag caactgcttt ttctgacatc cactctggag ctatacctac aactggaagg
    39601 tcacacatat ccatatctaa gtaattagct gctgctccaa ctaaatctag tatacgactt
    39661 atatcaacac aagaacccat atgtagtact ggtgggattc ctactaattt acagacagtt
    39721 gcaagacctt tacctgcata tttttcagcc gcatcagatt ccattaaacc aaacttagct
    39781 gctgctgaag ctccacaacc tgtagtaact actattatgt cgtttttgat taattctttc
    39841 attattgtta cgtgagcctc atttgaaact cctttagcat tgttacatcc aactacacca
    39901 gctgctcctc ttaatactcc agattttaaa cagtctgtta atggcttaac tgttcctgct
    39961 gggtctatat gagagtttac aactctgtct aattgattta ctactgcttc tacactatac
    40021 ccaactgttg cgcctgattt taattcaggt ataagtactt ttgatttatc tctattttta
    40081 aagtttaaaa tagcttcttt aactattgct ttagcatcat caagtgcttg ttcttctcta
    40141 aattcaatat aagttgaacc tgttatctta gcttttggag aagttgttac aaatttagta
    40201 tggtaacaat ctgcaactct agctaatgca gggaatatac attgaacgtc aactattaca
    40261 gcttcaactg ctccagttat tattgcaagt tcttgttgat ggaagtctcc tgctattttt
    40321 acaccatgtc tcattgtaat ctcattacct gtacaacaca tacctgctaa gtttattcca
    40381 tcagcaccaa cttctttagc taaagctact aagtctggct cttctgatgc taaaactatc
    40441 atttctgata atgctggctc atgtccatgt aatattatgt taactttatt ttcttctaat
    40501 actgctaaat tcgcctctgt tcttcttggc actggagttc caaataagat atcgcttaga
    40561 cgagtaccta tcatagaacc accccaacca tcagccatag aagttctaag actcatgtgt
    40621 attaaactat ctatatcacc agtacatcct atatgagtag agtgcattat tgttgcaatt
    40681 tctctatcta tagctcttgg ctctatagcg tattctttcc atattttttg tcttggttct
    40741 ggtgcatttt ttataaatct taatgtccca aaaggttttc caaattccat taatgcaatt
    40801 tctgccactt catgtgctac atcatagata tctctacctt ctgtctctac atcccattct
    40861 ttagctaaag taattaactt agcttcgtct cttattttat agtttccatt tggatcagct
    40921 aatgctaatg tatgtgctat atctctagca tggtctgaat gggcagctgt cccaccagca
    40981 accattcttg caaagtttct acctactata gtatgctcat ctgctccaca taaccccctt
    41041 ggagtcttag ggcttattct acatggtccc attccacata ttctacagca aacaccttga
    41101 agaccaaaac cacattgtgc cttaaagcct tcttttctat catacattgt ttctacgcct
    41161 tcttctctag ctttagctat catcttctgg ctgtttaaat caattgttaa cattttttca
    41221 tccatattcc tatccccttt ttttgatttt taaaatcccc caggtgactg acttcatgtc
    41281 ataaaataat aaaataaatg ctttctaagt tgattatata ctaattttca gtgctttgct
    41341 ttagatttaa atattttttt aacaaaatta gataatacat aaaaatttta ttttctacac
    41401 ttaatttaag ttgattttat cgcttgtttt tggctatttg ttaatatcta aacctacttt
    41461 tttgatactt cagtttaatt aaattatgtt ttgatttttt taaatcaaat ttgaaatttt
    41521 tctagtcatt tttatattat tgcagattgt ttgtgtattt attatctttc actactgttt
    41581 ggacttatta cagattttta ctccctaaac ttaattagta aaaacttctt agaatagcat
    41641 tttttggact aatttttttg aattttttta tttttcctac tttaatatta attatagctt
    41701 caaattgaac atttgtatat attcatttga agctataatt attttttata taaaactttt
    41761 ttaatacagc attaatttag acattttgac tattttttct actttaattc atttatttca
    41821 agcgatttat cctatttcat atttttagct acaatagccc catatgcaat agaatataat
    41881 ttagattcat gtgaatcagc tcttgacact aaaactatag gagcttttgc acccataatt
    41941 atacctgctg atttagagtc tgccatatat gtaaaggctt tccctattcc attaccaacc
    42001 tctattgttg gaaccaatat gatatctaca tctccagata cttcactttt aaaccctttt
    42061 atctcactag cttcttttga aacagctaaa tcaaaagcca gaggaccttc tacaattaca
    42121 ttttcaccaa attctccttc tttacaagct tttgctaaca tatctgcatc tacagttgct
    42181 tgcatcttag gatttacttt ctctttagct gccaaacatg caacttttac agtttccatt
    42241 cctaaagctt ttgcagcttt tatagaattt tttaatatct ttgccttttg ttcataatca
    42301 ggagctatat tcatacctcc atctgtagtt ataagtaatt tatggtattt ttctaactca
    42361 tataccataa catgacttaa taaactatca gttctaagac catagtcttt atttaacact
    42421 tcttttaata tgacagatgt atctaataat cctttcatta caaagtcggc ttctttactt
    42481 gatactaatt taactgctat ttctgcacac tctttgatat catccttatc cactattttg
    42541 ataccactta aatcaaatcc tatttctttt gatatttctc ttattttgcc ttcatttcca
    42601 accaatatag gagtaattat atttctttct acagcatcct ttattgccat taaaacttct
    42661 ttatcatgag ctgctgctac agagagtaca attttctctc ctcccttaag ttgttctaaa
    42721 atatcatcta atttttttat caaaagtaac ccctccctta tatatattta actaaataat
    42781 accatatttg ctataccttt tctcaattta ttttttatgt tataaagaat ttatataact
    42841 aaaagacagt ttaatatttt attctgggat ttcatcaata actcttaaaa gctttgcagg
    42901 aacaccacct acaatagtat tttctgcaac attttttgtt acaactgctc cagcagcgat
    42961 aattgcatta tctccaatag taactcctgg aactacagtt acattagcac caatccatac
    43021 attttttcct attgtgattg gagaaggata agttgtacca cgtttctctg gtgcaatacc
    43081 atggttaagt gtagccaata ccacattatg cccaatttgc gtattatctc caatctcaat
    43141 ccccccctgg tcttgaaaat ggcaactgct atttataaaa acattttttc ctattgtgat
    43201 gttttttcca caatctgtat aaaatggtgg aaacataaaa aatgattcat ctaccttttt
    43261 tccaattaac tcagaaaata aactacggac ttcttctggt tcatgataag aagaatttaa
    43321 cttacttgtg attttcattg cttcattact caacttaacc ataaattcat gtatttctga
    43381 tttactagaa acaggttttc tttgattgat atgtgttaaa aattcattga tattcatttt
    43441 atacatcctt tcaatatgtg ctttaattta tttgtacatt ctaaataata ttttaataaa
    43501 aattaattta atttattttt attaaaatat cattgcttct tttagtgtaa tatacaatta
    43561 tatctatatc aaatatctat agtaaatact taactatact taaaaagcat ataaattgat
    43621 ataattatat aatagataag ttaatattga ggagggataa ttatggaaat aagagtatta
    43681 agatattttt tagcaattgc aagagaagaa agtatatctg gtgcggcaac ctttttacat
    43741 ataacacaac caacactttc tagacaaatt atggaacttg aagaagagtt gggagttaaa
    43801 ttattggttc gaggaaatag aaaaatcacc ttaacaaatg aaggtaggct tttacataaa
    43861 agagcagaag aaattatatc attagttgat aaggttgaat tagaatttga caatcccaat
    43921 gagattatta gtggtgatat atatatagga agtggtgaaa ctgaagctat gcgacttata
    43981 gctagaactg cccatcattt tcatcaaaaa taccccaatg taagatatca cctttttagt
    44041 gggaatgctg atgatgtgac tgaaaaatta gacaagggac tgcttgattt tggtatttta
    44101 attgaacctg caaatataca gaagtatgat tatgcacaaa ttcctgctac tgatgtatgg
    44161 ggtttactaa tgcgtaaaga tagtgccctt gcatcacaaa gcacaataaa tccaaaagat
    44221 ttatgcaata tacctcttat tatttcaaga cagacacttg ttgataaagc aatttctaaa
    44281 tggcttcaag acagtaatga aaaactaaac attattgcaa catacaattt agtttataac
    44341 gcttctcttt tggtagaaga aggtctgggc tacgctctgt gtttagatag actcgtcaat
    44401 actacagaaa acagtaatct ttgttttaga cctctatctc ctcctttaga agctaaatta
    44461 aatattatat ggaaaaaaca tcaaatattt tcaaaggcag cagaaaaatt tttacttcaa
    44521 atacaaaaag atttttcttc tatcatgttg gatacatcta agttggatac acctaaagaa
    44581 aaataaatca taataaaagg tgagagtgtc tcaaaatgaa ctttgcactg tataaggcac
    44641 tcttttttat acaaagtaaa atatatgttc atcatttcag cactaaaaaa gaggcttatc
    44701 tctattttga gatagcccca cttaaccaat aaaattaaat acaacttaac aatatcctta
    44761 cttatttatt ctaagttctc ttagacaagc aatagtaatt tcatcttgtg gatttaattc
    44821 ataagctctt tctatatagt aagttgcatc atcaatatca ccattattta aataagccat
    44881 tcctaaattg caaagtattt ctgggtcttc ctttatctta gccgcttgtt caaaatactc
    44941 tattgcttta tctaaattat tcttataagc ctcacaaagc cccaattcca ctatagtatc
    45001 cacttgatta ggctttatta tcaatatctt ctcaagatac atcttagcct gctctatttc
    45061 gcccataccc ttatatccaa gtgctatcat aaacagcaag ttccaccaat ccatatgttc
    45121 ttcttctaat ggaagtaact tctctaatcc ttccttaaac ttaccttgga atacaagtgt
    45181 atatccttct tcatattgaa ctttaaagtc catcttgccc agattctctt gtacctcagc
    45241 tactaaatca gcatctaacc ctagccttaa cgcttcttcc caagtcagct tagtctttaa
    45301 atattgacct tgattataat aatggtagcc taagtgataa taacctaatg caaaattctc
    45361 atcaacatca attacctttt caagcttagc catggcttca agtaagaatg cattcattgc
    45421 cttatcatcc atatcttttt gatactgttt agctatctct tggcaaacaa tcgcatagtt
    45481 atacattgca tctaaatcat ctggatacat tgttataaat gcttttatat atattaaaga
    45541 atctgtatgt tcaccaatct caaagtattt tctcgacata tatcccatat acgatttcaa
    45601 atccaaatca atatcttttg ataaagcatt taaaaacttc tcatactctg cattgttttt
    45661 aaacttacta tctattccaa ttatatatat cattgcatct gctatagaca ttgaatttaa
    45721 cttatcttga gcagtctttt cttttatccc ttttactaat acctcatttt ttataggtac
    45781 atctaatcca ccttttggca cttcatagct ttttaattta aactctccac catgttttat
    45841 agttataaaa gctaattctt ctgctttttt tagcaaatat ttttctatct taaaatccat
    45901 tttatcctcc taaatactta aatctctact ttatgagttt tagatttctc aaatattttt
    45961 ttatcatcat actcaaataa gactacattt tctacttctc ttatttctgt atttatatca
    46021 ttttttaaat ccacaacatt aaatctaaat ggctcaaaat gaacatattt tattatatgt
    46081 ttagctggtt tatcaacaaa actaggtaaa tatgttgata agttatcatt atcttgtaaa
    46141 aatacatggc atctattctt aaaatcatcc acatctagct tattaaaaaa ctgtggtata
    46201 tttgatgttt tacccagtga tatatagtca tattttaata tatcgttgaa tacatcatta
    46261 tctatgttga ttttttcatt atagaaatcc atcaatattt tatataactg attctttcct
    46321 tgtgccaaat caaagtagcc attttcatca aaatacttgg caaaaacctc aaagaattta
    46381 aaagggcttt ttttataaaa tctgcctata atatatctca tagataacac aaagttttta
    46441 gaattgtagt atctctccag tatatcttct atatccttta gcttcaaagt ttcttcatag
    46501 cttatataat tattgtataa aaactcatat ggagcataat ccttatattt aaaaccatgc
    46561 tcatctgcaa cttttctcat acctgtccct ttaatcatct ttaaaaagcc taattgcaaa
    46621 tgctctatac ctagattaaa tacatcatta aaagaatttt caaagctctt ataatcttca
    46681 taaggcagtc ctgcaattaa gtctaagtgc tgatgtatat ttctataaga tgctacagtt
    46741 tgtacaacat gagacaactt tttaaaatca tcccttctac ctactgcatc aagtgttttt
    46801 tcattcgttg actgaacacc tatttcaaat tgaaaaagac cttctttaca atcagccaaa
    46861 aactcaagca tcttatcatc tatcaaatac gctgtaactt caaaatggta agttgtatat
    46921 ccattatcat ttttcataag gaaatccatg atttccatag ccacttttct atttgcattg
    46981 aatgtcctat ctataaactt tatctgtgat actcttgcat ctattaaagc ttttaaatca
    47041 ctctttatcc tgtctaaact aaaatacctt agacctggaa tagtagatga taaacagtat
    47101 tggcagttaa aagggcaacc tcttgatgat tcataataca ctattctatt ttcatattct
    47161 tttggattca agttttcata aggactagga atctcatcca aatcttgaag taattccata
    47221 ggcttattca ctattatctc attgccttgt ctatatacaa gtccatttac atcatttatt
    47281 tccatctttc cttgcaagtg aagtactaag tctctaaaaa ttaattctcc ctctccataa
    47341 agtatataat ccacaaaatc atactttttc attgcctcat aactatcata actcacttcc
    47401 ggtccgccca aagctatttt agtattaggt ctaatttttt ttagattatc acaaagttta
    47461 actatatctc ccacattcca gatatatgtt gagaataata ttatatcata ttcattttta
    47521 tatatatcct ttaaaatata gtccaaatcg ttatttatcg tgtattcttt catattaact
    47581 tctatcaaat ctcttacaaa ttcttttaga tatcttatag ccaaatttgt atgaataaac
    47641 ttggaattta atgttgttaa taaaattttc aaatatttct cacctctttt taagattata
    47701 ctgtcttttt gtacccctta taggttaaaa tagtatatgt aaagtctaaa taatatctat
    47761 ataattaatt tattaatttc aagtaatgtg aggaaaggaa gttttttatg gaattaattg
    47821 aaaaagtatc tatcaactct ttatcaaaaa gagatttact acttattata aagtctcttg
    47881 aatacactaa tgaaaatact caaattagtg atttcattga ccttaggaat aatatagtaa
    47941 aggaattatg ttttttaact gacactaagg aaaaagattt tttagattat ctagaaaaaa
    48001 atagcacact ctaataacct aaaccttttt aaactttaat atataaatac ctgatttaga
    48061 atttattttt atgcttaata tattatcatt aaaggttttc gacataactg ttatttgatt
    48121 aaatataggg tctattatag tattaccttc ttcaagtctt ataagacttc ttgggtctat
    48181 gcttttatta tatgccttaa ctccaatttt atattttctc aatttaacaa catattcgga
    48241 catagaaaaa ataaaatcta tattatcttc atttaattta ctatcttcac ccaaaatgga
    48301 taatatagat ttttttatgc tattaattcc ttcattttct acagttctat cttccatata
    48361 tacatcatat agaacattta aaacatttaa taaaatactt tcttcatcat aatcttcaga
    48421 tttcaaatca atgtacttat caattaattg tattgcttta atccttgcca tctgaaactt
    48481 tacaacatct gatttgtcta attctataga aaatcctatt atcttgtctt taatactctg
    48541 tagtaattcg acatactcta ttttactatt atttataaga ttgtgcataa tggaattata
    48601 caccacagaa cctaataaat aatcaaataa aagttcttct tttttaaaat aaataacata
    48661 cttttttatt agtttaataa cctcatcttc acttttttga atattagtat ttgataaaat
    48721 tagtggtaat aaaatattta aaaaattgat atttcctatt ttttctccat aaaatctata
    48781 gtaaataagt tcatctctca gtctattttc gtctatatac acacatcttt gcgttttatc
    48841 atctattaag gattttgtat ttattatagt taaatcttta agagccctac ttgcactact
    48901 aaaagaagat gtgtagactt tttttagtaa ttcgtcattc ataaatatat cctttctgaa
    48961 aaattgaatg taaataaatt gttattaaaa aaaaagagac agcccgttta agaagctgtc
    49021 atatataggg ggatagtagt gtacaaaaat cattgtaatc tactatctat atattattat
    49081 acgatactac ttttttatat gtaaagcatc agttttcgac atccctatat tttacagtta
    49141 tacaaattgc gtactaagtt caattttatt ctttttattt caacatttta tcatgctaca
    49201 ttctttcagg agcgtgcatt cccaacaatt tcagagaatt ttctaaagtt tgttttgttg
    49261 ctaaaactaa tgcaagtctt gcttttctta tttccaaatt ttctactaaa attggattgt
    49321 catgataaaa tttattaaac gcttgagcta aatctagcat aaatctagtc actatatgtg
    49381 gctcattttt ctttagagca agtaaaatat tcttattgaa tgattctata actctaagaa
    49441 cttctgcact atcttcatca gctaatagac tataatctat atcagaagtc acttctactt
    49501 ctgcttttct taaaactgca cagcatctag catgtgtata ctgaacatat ggtccagtct
    49561 caccatcaaa actaagcgtt ctttcccaag agaaggtata gtctttaatt ctactattag
    49621 ataactcttg gaatactact gccccaacac ctacttgctt agatatttca tccacatttt
    49681 tagcatttgg gtttttagct agtataattt ccttagtttt atctatagct tgatttaaag
    49741 catcttctaa aaatactact cttccttttc tagtcgacat agtaccttct tcaagtgcaa
    49801 ccataccaaa tccaacatga attaaatctt ttgcccaatc atatcccata agctcaatta
    49861 ccttaaacca ttgttggaaa tgtaagtttt gttgagaacc aactacatat atacacttgt
    49921 caaaatcata agtttctttt ctatatattg cagcagctaa atctcttgtc atatataacg
    49981 ttgagccatc attcttagta ataagtgctg gtggcatttt atactcttct aaatcaacaa
    50041 ttcttgctcc tttagactct tttaaaagat ttttctcttc taatagttcg ataactctgt
    50101 tcattttatc agaataaaaa ctctctcctg ctaaagaatc aaattctata tctaaaagtt
    50161 tatacactct gttaaactct tttaaactct catttctaaa ccattgccat aattcttgtg
    50221 cttcttcgtc accattttct aatttattaa accatgctct tgcttcatct tccatttctg
    50281 ggtgttgttc agcttcatca tggaatctta cgtataattt tagtaattct ggaattggat
    50341 tagactctac tacttctttt tctccccact ttttaaaggc cactattaac ttaccaaact
    50401 gagttccata gtcacctaaa tgatttattc ttatagtctt gtatccttgc gaatcatata
    50461 tcttatataa agcattacct ataactgtag ttcttatgtg ccctatatgg aaaggttttg
    50521 ctatatttgg agaagaatat tcaactatta cagttttatc ttttccaaaa tcactatgtc
    50581 cgtaattttc tttttcatct aaaacttttt taattacagt ttctgctaat tgagatttat
    50641 ttacaaagaa atttacatat ccgcctaatt gtattacttt tgttatctct ccacttggtt
    50701 ctatactttc cgccaattca cttgctatca tgtttggagc ttttctaaac actttggcta
    50761 atttaaagca tggaaaagca tagtccccca tatccttatt tggtggaact tctataagtg
    50821 cctctatctc ttctttcgat aaatcttcta ttttttcttt aaggcaattt gatattgcca
    50881 ccttaaaatc ttgcattctg ttgctcctct ctacattgtt cctggtttat attgtgctta
    50941 tctctttatg ttaaatcaaa tttcgtgttt tttcaagtat ttcattacag tgtaaaacac
    51001 tttagtaatt atgttttctg tatatattaa aaatactata attgcagttt ttaatcacac
    51061 tattattgat tttttataag acttttttct attttattgt ttcttgtact atccagttga
    51121 ctttatatac tttttttaat cttttatcta ttttcatacg tcaatgctaa ttttatatac
    51181 ttttctttta atcttttatt aatttttata catcaatgtt aactttatat aagttttttt
    51241 aatcttttat ctgttttcat acatcaatgt cagctttata tacttttttt acttttttat
    51301 ctatttttat acatcaatat tagctttata tacttttttt aatcttttat ctattttcta
    51361 taaatctttt tcactcttaa cttttattcc ttcttcttct aatagccttg ctgttatacc
    51421 tttccctggt atctttttac ctgaaaaact accatcatat attagattac atccacaaga
    51481 ggggctaccc tcttttagta ttgcttcgct acatacatat aaatttgcta tttttagagt
    51541 ttcattagcc cctcttacaa agttttctgt tacatcctta ttatctttag taattacttt
    51601 tccatcaact atctcagcag gtggtcttgg tgtagataga ccacctaact gctctgggca
    51661 tacaagtgta aattctttgc cttttaaata ttccttgact ttttcattat cattattatc
    51721 gccactatat ttacaattta ttcctattaa acatgcagat accaatatca taaaaatgtg
    51781 ctcctataca aattcttaat atattctcat acaaattact aatgtattct tatacaaatt
    51841 attttaattt aacaattgtt actcctgctc caccttctcc atattgacca cctctttgtg
    51901 attttacatg tctatttctt cttaaaatgt cttgtagccc tgcttttaat actcctgtac
    51961 ctattccatg tattactgtt acagactcta aacctgccac atatgcatca tcaagatact
    52021 tttctacttc cattatagct tcttctaggt ttaaccctct aaggtcaact tcatttttta
    52081 cactaccaga ttttgacctt ataatgtttc tcgttgattt agttacattt gttgaaacat
    52141 ctttgcgtgt tttttgtaaa gacttaaaag gtagagtcat cttcattata ccaatttgca
    52201 ctactgcttc ttttctcttt ttatctactg aaactacact accattttga tttaaagtta
    52261 ttaccttaac ttcttcacct ggctttaagt cttttatctc cttatttgat acctttggaa
    52321 ctatcatact ttttactgtt ggctgaagac ttcccataga gctggtcaat tcttttctaa
    52381 gttcttctat cttacgattc ttttcttttg aagctctttc ttgttcaaga cttctaagtt
    52441 cttttattat aatatctact tcttctttag cttgtcttat aattgaaaat gcttctgatt
    52501 tagctttttc aatcatctta tctctttgac tgaccaattt ttcaagcttt tcgtcatact
    52561 ctactttcag tttttcaatc tcttctttta atctttcagc ttcttctcta tcttctactg
    52621 ctttgattct attcttttca acattctgta acacatcttc taaagctatg ttttctgtat
    52681 ttatatattc tttagcacgt gaaatcacat aatcacttag cccaagtttt ctagaaatct
    52741 caaacgcatt tgattttccg ggaacaccta taagtaactt atatgttggg cttaaagttt
    52801 ctatatcaaa ttcaactgct gcattttcta cacctggttt agtaagagca taatttttaa
    52861 gctcactata gtgagtggta gctatacatt tagctcctac agaatttata tcttctaata
    52921 ctgctattgc aagggcagct ccttcaacag ggtctgttcc cgctcctaac tcgtcaaata
    52981 taactaaaga gtctgctgtt acgttttgta atatagatac tatattcgtc atatgagatg
    53041 aaaatgttga tagactctgt tcaatactct gctcatctcc tatatctgca aatacattat
    53101 catatacaca catactactt ccataatcag caggtatatg aagtccactt tgtgtcataa
    53161 gtgcaaatag acctacagtt tttattgtaa cagtcttacc acctgtgttt ggacctgtta
    53221 tcaccaatgt atggaagtct cttcctaaat aaattgtatt tgctactacc ttctttttat
    53281 ccagcagtgg atgtcttcca ttttttatgt ttaaatattt atcctcattt aaagttggtt
    53341 ctattcctct catctgtata gaaagttttc cctttgaaaa tgcaaagtcc aatcttccaa
    53401 gtatctcttg attagaaatt aagtcttcac taacttctcc aaccatagca gaaagctctg
    53461 ataaaattct ttctatttct tcttgctcac ctagttttaa ctgtctcaat tcattgttca
    53521 tctctactat agtcattggc tctataaaaa gtgtcgcacc agaagatgac tggtcatgca
    53581 ctataccagc tacttgcgac ctatattcag attttacagg tactacaaat ctatcacctc
    53641 ttagagaaat tattgcatct tgtaagtact tttgataagt agttgaactt attattgaat
    53701 ttaattttga cctaatggat tgattttttt gggctattct tcttctgata tctctaagtg
    53761 tacttgaagc attgtcagat atttctactt cactaattat agcattgtat atctggtctt
    53821 ctatgtcctt gtatacatat agagaatttg atagagattg tattattgga taattaaaat
    53881 cttcttcatc actactagat aagctatttc ttaatcttct agccactctt aaggtatctg
    53941 ctatcataat cagacttcct gggtcaagag aagcacctat ataagctctc tttaccttat
    54001 cttctatatc atgaatacct tccaacccta ctgaacctct ctttataata attgattgag
    54061 cttcactagt ttcttctagc atagatttca cttctacaaa atcagtattt ggtactaaat
    54121 tttctatata ttttagacct aaagaagatg aggctttctt ctttaacaaa tctattattt
    54181 tattgtattc tagaactctt aacgatttct cattcatttt ctatattcac catcctcgat
    54241 attttaactt ttttatttaa ttttaaaaat gtatgttttc aagcatttcc aataaaaact
    54301 gcaagtttta tttctttttc atttctaaaa ttattatatt ttttctcttt tagatatatt
    54361 ttacacatat tttgtattat actatgtatg tagctatcta tatattatac ataaacatgc
    54421 tttatttgta atataatctg ttaaaaaatc ttctttaata tttttaaagg agtttatttt
    54481 agcttataaa aaaaaatagc tacttatata gataaattaa ggaggaacat gagtcatggg
    54541 aaacgtatta gaaggattaa aaccagaaag tgtttttaaa aactttgaga aaatatctca
    54601 aattcctaga ggttctggaa atgaaaaagg cataagtgat tttttactta gctttggtaa
    54661 aaatttaggt cttgaaacta ttcaggatga aagcttaaac ataataataa gaaagcctgc
    54721 tacaaaagga tatgaaaatt gtccaggtgt agttcttcaa ggacatatgg atatggtttg
    54781 cgaaaaagaa aagaatgttg aacatgattt tcttaaagac cctataaaat taagaattga
    54841 tggtgacatg atatatgcta ctggaactac tctaggagct gacaatggta tagctgttgc
    54901 tatgggtatg gctatattag aagataatac tttagagcat cctgcactag aagttttagt
    54961 tactgttaat gaagaagatg gtatgaatgg tgcagatgca ctagacccta gcttaattaa
    55021 aggtcaatat atcctaaata tggactccga ggaggaagga tacttattag ttagctgtgc
    55081 tggtggtaaa acttgtgttg ttagtttacc tgttgaatac aaggaagtta aaggtgacaa
    55141 acaaggatta ttagttgagg ttactggact attaggtgga cactctggta tggaaattgt
    55201 tttacaaaga gcaaatgcta acaaagcaat agctagagta ttaagtgtat tgaatgttga
    55261 ttatgaatta gcttctgttg atggtggtac taagcacaat gctatacctc gtgaagctaa
    55321 atgtgtaata gctgttaata aagctgatgt tgaatctgct aaaaaacaaa taaatgacat
    55381 tttaactgca tttaaacatg agtttactac ttctgaccct ggcatgactt actcagtagc
    55441 tgaaacttct gtagacaaag ttcttactaa agattgtaaa gaaaaagttg ttcaaatgtc
    55501 ttgcttaact cctcatggag ttcaatcagt aagtcttgat atagaaggat tagttgaaag
    55561 ttctactaac ttcgctataa tcgaaactaa agaatctact attgaatttt taacttctgt
    55621 tagaagttct gttatgtcta ttagagatga aatcgctgat agaataagac ttttggctca
    55681 agctttaggt gctaattacg accttatagc tcaataccca gcttgggaat ttaaaaaagg
    55741 ctcaaaatta gaaaaaatat gcagtgaaac ttatgagaaa ttaactggta aagttcctac
    55801 tgttatggct ctacatgctg gtcttgagtg tggattatta cttgataaac ttcctcatgc
    55861 tgaagctatc tctataggac cagatatgtt tgatgttcat actccaaatg aacatgttag
    55921 tatcccttct gtagctaatg tttgggatta tgttattgaa atattaaaat ctatgaacca
    55981 atattaaatt taagttttga ttctaagtag attttaattt taagtaatct atataaaatt
    56041 aactctgtaa attaatacta ctgttagtat aatatttact ttcaatatta acttatagaa
    56101 ataaagccta atgatagata ataacatgta tcattaggct ttattgtaat ttaaatatta
    56161 ttaagcttta ttctgtttta aaattaattc ttatactatt ttaaatctat ttcttccttg
    56221 tctcttagaa tcataaagag cattatctgc tatttgatac agttcatcat actcttgacc
    56281 aggctttgct ataactaccc ccatactaat agataaactt tctccatttg gcaacttaat
    56341 gagatgaata tgattgtata catcattaca tttattaata agttcttcct tatttattat
    56401 atcattgata taaacactaa attcatctcc acccaaacga cctactatat cattttttgc
    56461 aaaactttca tcaagtacct ttgcaacatc aataagtact ttatctccta acaagtgacc
    56521 ataagtatca ttaatcgact taaaataatc aatatctata atcaataaag caccattttt
    56581 ttcagttgat aaattcaaca aattttgtgc agttaatttt ctaatatatg ctgcattata
    56641 aatattggtt aaactatccc tttgagcctt atctaacaat ctattctttt cctctttttc
    56701 agaatcaata ttacatattt tcccgattac atacattggt atttttctat cattataaat
    56761 tatttttgaa gtaactctaa tccaatgaaa agtatcatca agcataatag aatacatttc
    56821 agttgttcca ttttcttgtg attttattac atcaaaaaac tttttattat attctttcct
    56881 tattttatct ctttcatttt ctatattgcc attttcactt tccttatcat ttttatccaa
    56941 accataataa gtctcaatac ccttttctgg catagaaatt atcaatttat cttgcttata
    57001 atcatactca aaaaagtatt cacttgtcat ttcatacaac tgagtatgtt ttttgagttc
    57061 taaagatatc ttctcgttca tacttgtacg agtatgcaaa ccaaatgcca taaatccaat
    57121 aatacaaatt aataaactgg ctacaataaa aatagtctca attggattct gttgcataat
    57181 ataaagcaac gagaagtctt gtctatatgt agtattttgg tagacaattg attgcatttt
    57241 ttctgatgaa atacttataa tagctttatt aaatatattc aataattctt gtttatcttt
    57301 tttaattata ccaaaacaaa tactctttgc tttataagtt tgaggaacta gacgcacatt
    57361 tttatactta ggttgattca tatagtactg catagaataa ccttcgccat aagtgtaatc
    57421 tgcatagcct ttattaacag cttctataca ctcttctaaa ttatcatacc aaactatatt
    57481 ctcttcaaac tgaccataat aattaatacc tttggctaga gctacacgtt ttccatctaa
    57541 actattctca tttaatttat tattaatcat catagtatat tgagtagaaa gaaaaggccg
    57601 agacataact atattatgtt cacgagagta ttcataatca taaatcattc ctgctacaat
    57661 atcaatttca cgctttttag tcatgttttc taaatcttct tgagatttta caggcacaag
    57721 ttcaaattct aagcctgttt tatcacatat atgatttaac aaatcaattg atatacctct
    57781 taattcacct gtatctttat ctatattttg aaatggtggt ttgtttaaaa gtactcctac
    57841 ttttaatttt tctgtctctt taatgtaatc agtttcttca tcagataaaa acatttgatt
    57901 gttctctggt gtaaagtatt tttcatataa agtagttgaa aaatatgggt ctttctgttc
    57961 aatattatta attgctgagt taagcttttg agttaaattt gtactacctt taggagtaat
    58021 gaaataaaat ggttttgatg aaaattttgc tacagtacgt acaccttcta gtgggttcat
    58081 actaacatta agaagtacat ctgctttacc actctttagt gcttctagtt gctcatcatt
    58141 tgttttacaa tataccaatt caggtgacat aagattcatt ttacaatact catctaactc
    58201 tttaagtcta gtatcagaat tttctattac agcaatacgg atttttttca catcttgtga
    58261 actcattatg ttatcaatat ctgtattttc atataatgct tgtagtactg tatttgctac
    58321 tccataacta tttcctgcat aattatatat tttttgcatt ggctcattgt ataacatacc
    58381 tcccattata tcaatttcac cattttctaa catcttcatt aattttgtaa tagattcatt
    58441 tatctctcca ggaacttgaa caaattcata attccatcca gtatactgtg caatttctag
    58501 taaatattca tatgtatacc caaaatagtt accttgctca tctatttcag ttaaaccttt
    58561 ttgaatagga taagcaacac gaatggtacg attatcatta cctatagcgt tgataccaaa
    58621 agaaaatatg caagaagtga atatagatgt aattacaata attaataaaa gtaatttttt
    58681 taaattcact acacaatcct ccatatagtt aagatttaca gtataaatta ctgcataaac
    58741 ttgtagacat gtaaaaatag attagtatat aaaaggatat ataaatctac ttagaaattt
    58801 aaatatcctt aatatataca atatatttaa attataactc atttatatgt ctattacaac
    58861 aaatttaccc tgctaaatac ctactctata tcccataatt ttacaagttg tagtgaagtt
    58921 taagttctat ctcacctcaa atttagcagt gtttctattt acagttcttt tatatttcac
    58981 atctggataa cccaatataa atgaagtgat aacagcttcg ttctcgctca ttcctaacag
    59041 ttcttttatc tttggctcta catttgatgc cataacaaaa aatccattat aacaaattcc
    59101 taatccttga gaatatgcca taagttccat attagatgca gccagtcctc catctacata
    59161 agcacttcca cctagagaca tatctcctac aactaccata agtagtgggg catcataaaa
    59221 taaagaatca tttccgttct ctttatatct tttgtacatt ttcttaaatg tatttttata
    59281 gatagaacgt acagggtcat tgtcatcagt atctaatgcc aaattataaa gaccttgtat
    59341 tgccagttct ttcacttctt ttagcttttc ttttactaat atgtatctta taggctgtct
    59401 attaccacct gttggtgtgt atctaccagc ttccactatg ttttttattt tttcttcttc
    59461 tacctctata tctttatatt gtcttatact tcttctaaat ttaataaaat tcaataactt
    59521 ttcgggttct aattcaaaac tttcacgatt gtattctatt acttcatttt tatcaaatcc
    59581 ttgcatttct attgcatctg ttgggcaaat tgccatacag tgaccacagt taatacagag
    59641 aatattttta acctttgctt tatcatcctt aacttttatt gcatttacaa gacagtctga
    59701 ttcacacatc ccacagccta cacatttttc taaatttatg tctatcattc caaattattc
    59761 ctccttttaa aaaatttact ttagtataaa acaaatagtt gtctaagtca atttttattt
    59821 ttttaacaat atactagcct taactacact ttaatggaga aaaatataca taaattggaa
    59881 cttaaattag aacttattca aagtaaaaga taaaagaagt agatgtctaa aagtgaactt
    59941 tttagttaat tagatattct ttattgtatg aaatcaagtg gatatttatc atttcaacaa
    60001 taaaaaaggc ttacctctat tttggatatg tccctgtcaa gtagacagac taaaaaaagg
    60061 ctatctttat gcactgagat agcccctttt cccaactact attgtttatc ttaatattag
    60121 tcaataaatt taatttgcta taacctgtaa gttacatttt aatttgatgt aactttaaat
    60181 tacattttta agtaatatct ttaaattata ttcttaaaat atatcattaa actacatttt
    60241 aattaatcta aaaactatat cttaattaat ctaagaaact tacattataa tcttttagtt
    60301 tcccattatc atatacaatc tttgcactaa agatatttcc agtcttagcc caatttaaga
    60361 acagcctgtc accttcccac atatttaaat cgaatatttt atcattatca acccaaatta
    60421 aatttccttc tttacaatca tttaaatctt tttcagataa ttcaccttca aactcatctg
    60481 agaaatacaa aaacatatac caatcttctt catctttaaa ttttggaaaa gttatcaacc
    60541 ctttatatga caatgattta agagtaagac cagtctcttc ttttacttct ctaattacac
    60601 attcttctgg agtctctcct tgctcaaact ttcctccaac tcctacatac ttaccttcat
    60661 gtatatcatc tttcttttta tttctgtaca acatcaaagt cttgtcatct ttttctatat
    60721 aacaaatagt tgtaagtttc atgtatctcg ccttgctttc tctttatatt ctcaactaat
    60781 tattttcagc actttcaagc acatcactta tattcttcat tgcattaaga gaaagctctg
    60841 caaattcatc aaatggaatc cctatatctt ctatacttct cattgcatct ctatttacat
    60901 ttgaagcaaa tctcgtttct ttcattcttt taataacaga cttagtcttt acactggtaa
    60961 gttttttatc tggataaact aaagctatag cagttataaa cccagttata gggtctgctg
    61021 catatattgc tttttccatt aggctagttc ttgtaactcc cattccatca tgatgaccaa
    61081 gtattgctct atacatttct tcgtcaccga agttttcttt tttaagcagt tctactgttg
    61141 ttgtagcatg tccctcacat atttctcctg gctttcttcc aactatatct gaatccaaat
    61201 catgtaaaag acctgccact gcccactctt cttctttttc tggctctagt cttttggcta
    61261 attctctcat aacagcttca acagcataac aatgttttct cattctatca gtttctaaat
    61321 acttaaataa aatttctaaa gcttcttctc tcattttcac caatccccct gttttttcac
    61381 atatatgata tttttatata ttaatacaca taaattcaag tttttatata tattcatgta
    61441 tatttttcat gttatatcta ttctacacct ttatagtagt gtcctgcaac tgttccaatt
    61501 tcatctaatt tcttatacaa gtctgtagcc ctttttccaa gtttataatt attactcaat
    61561 acctcaatgt gagcttcaat aaccttttta accatctcac tatcttcaac tgtaaaatct
    61621 atcctaaata catctattcc aatttcctct aactcatata gattgtccaa catacaagta
    61681 accttcgaat tgtaaattgt acttctacag aatatatctt gtgttatcct ataacttgca
    61741 tccgtaaagt ctcttaaagc ataactactt tccttacatt tagcaactct cttgtccttt
    61801 ttacaatccc taacaactac tcccatagga cagtattctg taaccatcat aggagcatat
    61861 ccatatatta ctgcttcaat ttctacatct gtatatttta aagtttgttt aatttcattt
    61921 aagtttagtt cttgtgacaa acaaactgta cttgctccct cttctttgaa gtggtttata
    61981 ctttcactat tgaatgcatt taagtagcaa tctatattta agtctttttt cttaaagtat
    62041 tccatagagc cccagttacc aatttgaacc ttattaattc cttttatatt atccaatatc
    62101 tcaaattgct tgtactcttt atttctaaca atccttggtg ctgaatagat taattttttg
    62161 tcagtgactg ctgtattaga gtggttctta ttcaaagtat ctattttact tgtatttgac
    62221 aaagtatata tgttatttgt atttgaaccc attagttctt taggaatacc taaagatgaa
    62281 gaagtatata aatccataga aatacttgaa tctatagaaa tacttgagac catttgtaaa
    62341 gcttttgata atgtatttat atcttcataa tagattaaat ctacattata atttaatact
    62401 gcttctaatt gctccaagtt cttaacttta actctaatct taggaactgt atcaagtttt
    62461 ttctgtttat ttactctatt tggctggtat tttaatctat tcttcttaaa ttttctatct
    62521 tttatcttta ttctttcttc atctaaaagc tcaatacaat cccttctcat ctgatttaac
    62581 acacttatag gcatacttac accttcatcc aggtcaattt ctatctcttt taacttatat
    62641 ggtgtatttc caagcttttg aatttgtgtt tctatttttt ctttacttaa agccaccttt
    62701 atagcttttt caactatttt gtctccttct accaccgatt tatttccaca aaaatcactt
    62761 ataaaaagct ctggcttttt acctagttta atactcactt ttgcacgtat attagttttt
    62821 atgaactctt tatcttgctg aaatgtagct tgtactctat ccatcaattc gctgtcagaa
    62881 gtcttatata taagctgatt tctctttgct tctccaacaa aatctaattc tatcaaatcc
    62941 cctttatgtg ctattgtagt tatctctcca tcctttatta ttcttccaat agtacctcca
    63001 cctaagttaa ttccatcacc tttcttaagc gtattttcta gcattactct aagtcttttg
    63061 ttctttctat tataatctaa aacttttcca atatatagcc cttgattatt aggtctatta
    63121 gagttcatta tatccttacc aacttcacct aatatatatc cttttgtaaa ctttctatta
    63181 aatatggtat acaaattatt tattatttcc tcagaaatac ttattttttt cttatctaca
    63241 aattcattaa tcgcttctct atatcctgaa accacagtag ctacatactc tggctttttc
    63301 attctacctt caatttttaa agataataca cctgcttcaa ttattttatc caaatcttct
    63361 atagtactta aatctctagg actcaacaaa taatctccat tactttttac tacttctcct
    63421 gtatttatat ctatcaactc atacttttgt ctacatggct gtgcacacct acctctattt
    63481 cccgacctat tgcctatcat actactcata aggcattgac ccgagtaaca tacacataaa
    63541 gctccatgta caaaaacttc tatatctaca gttgtatttt cacaaatcca ttttatctca
    63601 tcaacattta attctcttgc taaaacaact ctatcaaacc ctacactctg aaggtatttt
    63661 acatcttcta aggaatgtgc taccatttga gtgcttgcat gtagttcaaa gtcaggtaat
    63721 agttttttta taagtcttgc cattcctata tcctgtacta ttaaagcatc aatatctata
    63781 tcgtataaaa attttacata ctctacaaaa tcttctattt catactgttt tatcaatgta
    63841 tttacagtaa caaagacttg cactcccctt atatgagagt atttaacagc ctcaatcagc
    63901 tcatttctat caaagttatt tgcagatgct ctagcactaa agtctttacc acctaaatac
    63961 acagcatttg ctccgttttg aactgctgct tttaatgaat caaaagagcc tactggtgca
    64021 agtaattcta tttttttcat atttttctcc tctatttata cttgttaggt taatataatt
    64081 atatctataa cttaaatatt tgtattaaat ttatatattt cttactatat tttaaattct
    64141 tcaatcaatt tatatattag ttatttatat ttggttcatt tgagt
//
LOCUS       DAMJYR010000007        58424 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.007, whole genome shotgun sequence.
ACCESSION   DAMJYR010000007 DAMJYR010000000
VERSION     DAMJYR010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 58424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 58424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..58424
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.007"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            202..639
                     /gene="spoVAC"
                     /locus_tag="PC471_000451"
     CDS             202..639
                     /gene="spoVAC"
                     /locus_tag="PC471_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein AC"
                     /protein_id="HDF2787592.1"
                     /translation="MDKNYKKYVDQISPKPTYLKNYTLAFIVGGIICMIGQAINDLYM
                     KVGGLDKLGASSATSITLIFIGAFLTGLGVYDLIGKRAGAGSIIPITGFANSIVSPAM
                     EYKREGYVLGVGANLFKIAGPVLVYGIGSSILCGIIYYIFKMF"
     gene            806..1819
                     /gene="spoVAD"
                     /locus_tag="PC471_000452"
     CDS             806..1819
                     /gene="spoVAD"
                     /locus_tag="PC471_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein AD"
                     /protein_id="HDF2787593.1"
                     /translation="MKNKRIGKRTVKLENKPTIISTGTIVGPKEGEGPLKDYFDMIMT
                     DDLYGEKTWELAESKMVETASQQAIQKAGKKLSDVNYMLGGDLINQIVPASFAARELA
                     IPFLGIYGACSTMAEGLCIGSMLIDGGFADLVLSGTSSHYCTAERQFRFPLELGNQKP
                     MTAQWTVTGAGSVLLAPNGDGPKVKYVTVGKVIDEGIDDGNNMGPAMAPAAIDTIYSY
                     FEDTNDDPNSFDIIATGDLGTLGKQIAEDFLKEKGVDISKVYTDCGIEMFNLKEQDVH
                     CGGSGCGCSATVFAGYIYDKLRKKEFNKVMLVSTGALLSPTSTLQKQTIPCVAHAVVI
                     VNE"
     gene            1833..2195
                     /gene="spoVAE"
                     /locus_tag="PC471_000453"
     CDS             1833..2195
                     /gene="spoVAE"
                     /locus_tag="PC471_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein AE"
                     /protein_id="HDF2787594.1"
                     /translation="MLMDYVRVFIVGGIICLIAQVMMDYFKMQTPYVMVTYVTTGVVL
                     TFLGLYEPLVKFAAAGATVPIIGFGYSLATGVIKSIQSDGFLGIFLGGTTATAGGIAA
                     AIFFGYIMSVVFTPKAKP"
     gene            2378..3394
                     /locus_tag="PC471_000454"
     CDS             2378..3394
                     /locus_tag="PC471_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDF2787595.1"
                     /translation="MVTIKDIARNLGISYSTVSRCLNNNPNVSEKTKKKVVEEANRLG
                     FHFNVNARNLAKKETNRIGVIFSNNFNHQDTRKFFSDIMDSSINSIETNKYDFIIQPN
                     NNISGDSNVYKMVNGQMVDGLVIVSQSIKKEEYDFLKDNNFPHVFIYFKPSFIGEVDN
                     FFWDDNVYGGYIATKHLIDHGHKDIITVTSDDKSSKMHEDRTKGYLNAMEEANLKTEV
                     IKSKMDFESQVEFLEKYIDKIKKASAIFVQQDVPAISIIQELKTTYGINVPEDISIIG
                     YNNIELISYFRSHLSTIDDPREQVIKNGVDSLVNIINKKSTEHIPRKLYPRLIIRNSV
                     KRIK"
     gene            3769..4611
                     /locus_tag="PC471_000455"
     CDS             3769..4611
                     /locus_tag="PC471_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3100 domain-containing protein"
                     /protein_id="HDF2787596.1"
                     /translation="MKEKAGIRNAIIAAFIITILAELIGPISFKVMGINVTLLSILWA
                     IFIGMAVSPHLLGRVIPALKKFIGDNEINVSPYLLSLTLYPLGIMFGINAGPKVGILL
                     QAGPALLLQEFGHMGTMLIALPVGVALGLGRSALGGTFSLCRDTALGIIGDKYGLNSP
                     EGIGTLGTYISGSIFGTLLFSFLAPVGVLLGFHPYALAMASGMGSGSMMGAATASLMN
                     TVPQMSEQILAYSATSGLITAVTGIYIELFLSLPVANYYYSKVAPVIERFRGRKTRRT
                     SNKL"
     gene            4626..5090
                     /locus_tag="PC471_000456"
     CDS             4626..5090
                     /locus_tag="PC471_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2787597.1"
                     /translation="MEKTFKTIMEQIKIVLFIYIIICFGQMIALKTQAQDLLIGSVSG
                     FLLVIIAIIIKNFVKKPNLPGFAWATLVAFVLSLPISPVGDIIATNVGKINFMSTVTP
                     LLAFAGISVGNQLDILKTLSWKLVVISFIVMTSTYFGSAFISQTVLRLNGML"
     gene            5077..6249
                     /locus_tag="PC471_000457"
     CDS             5077..6249
                     /locus_tag="PC471_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HDF2787598.1"
                     /translation="MECFKEVVNMNELKAKIKEEIKLLSEEKKTSFEKVSDYLFSNPE
                     LAFEEYKSQKALCDLLEENEFNVTKGVGGLETSFEAVYSNGTNGKTVAFLAEYDALPG
                     MGHACGHNIIGTSSVGAGIILKEIMKKHNIEGTVKVFGTPAEERVGGKITMIKEGVFN
                     NVDAALILHPSDASMPDDISFAQVNLKFDFTGKASHAAAFPWEGKSALSGVIALFNSV
                     NSMRLHLKDYARVHGIITDGGSIHNIIPEKSTAIFNVRALSIEYLNEICEMLKNCAKG
                     AAISTGTNVEIVQLDEIYKEIKNDSELVNIVRQNFEVLGEDYVERDLSQGIGSTDTGN
                     LTHEIPAIQAYIKLKENTATHTDEFAVAAGGEEGRVALIKAIKVLAMCGVDILYSK"
     gene            6293..7228
                     /locus_tag="PC471_000458"
     CDS             6293..7228
                     /locus_tag="PC471_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1177 domain-containing protein"
                     /protein_id="HDF2787599.1"
                     /translation="MILKQVIEVYDILDKANANGEEVKTYLQGYGEVDVTVKELSSSK
                     GSTDLVKLTIPGKNGKLKGGDAPTLGILGRLGGIGARPEVIGFVSDGDGALVAIAVAA
                     KLLDMQRKGDILDGDVVISTHICPDAPTKEHYPTPFMDSPIDMMTMNENEVDSSCDAI
                     LSIDTTKGNRVINTRGFAISPTVKEGYILKTSDNLLDIMQTVTGKSPFVFPLSIQDIT
                     PYGNNLYHLNSILQPAVATDAPIVGVAITTEMPVAGCATGATNFSDLDSAGRFAIEVA
                     KLFGRNKCDFYDKEEWEMLIKRYGKLNRFQTFGIQ"
     gene            complement(7502..10195)
                     /locus_tag="PC471_000459"
     CDS             complement(7502..10195)
                     /locus_tag="PC471_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HDF2787600.1"
                     /translation="MNNDNDKNGNKIRRKKVSSSGNTNKPINRTSANKTRSTKNKKKS
                     KKSDKFKKLRVFGIVFLVLLVVGTAGTAGLVFASLRDVTPVTEAVLDKQTNQTTTIKY
                     ANGKTLSTAPSVNKKTPVPLDKISPYLQHAVIAIEDERFYEHKGVDIKGLFRSVLKTL
                     TGTKQGGSTIPMQVSKMLLTTEQQTIPRKIKDIYYAHEMSKTVSKDKILETYLNNFFV
                     GRGLAGAEAGARGYFDKSAADLTLAESALLAGSTKNPSRFSAYKTSKLEGNETKEDLE
                     NKLLFFVNTTDDDLDDPTQVDFDMIEKIKSWELISNDTYRQLKAGTLVVRKAVSNPEA
                     KKRQEIVLKKMLELKYIKQSEYDEAIKAKIEIKLPQSSDKVSSSVEDLIESEVINALM
                     EQGHTNDEAQNLFYNGGLIVNTTIDPKMQDALEEEFDRNSNFPGHMVGPDGVSQPQAA
                     MVILDYKNGEIRALAGGRNISGRKTLNRATNPHQPGSSIKPLAIYTPAIDTLKITQAT
                     ALSDSRGGYKFEENNKWNPRTTTAGHGSMSLRKALAKSSNTIAVKTAEMLGDSYDECV
                     DIMMDYLKNFGITTLKNNHSGSSEASDRKFPSLTLGGMANGITPLQMAAAYGTLANHG
                     IYVEPSIFTTITTFDGQLLVKNAPEEHKVVDPEVAYVVTDMLESVITEGTGGVATLPK
                     GMPVAGKTGTTNSAYDAWFVGYTPYYVGATYIGDDAGRKDDSGNTIKRREVPHGSTST
                     AKLWEKIMEKIHANLTVTEFEVPKNVYFTKINLEDGGKQSSGSKAAFIEGTAPTKVSS
                     QPSSEDTKKPDNNQPEENNNNNNNTGDNGGGSTPPDNGGNNGGGSTTPPDNGGNNGGG
                     STTPPDNGGNNGGGSTTPPDGGGNNGGGSTTPPDGGGTPAT"
     gene            10409..11107
                     /gene="yunB"
                     /locus_tag="PC471_000460"
     CDS             10409..11107
                     /gene="yunB"
                     /locus_tag="PC471_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein YunB"
                     /protein_id="HDF2787601.1"
                     /translation="MNRIVDRKTKNEFRKIIAIFLVILFLSVFIGSFIYIDKTLRPTI
                     TVLAETKALELANRSINKAVAEMVEGKINYEDLMDIQLDNNGKITMIQANTIMMNEIA
                     SAIALEIQDELKKDKTASSYIPIGTALGSPILAKYGPKLEVSIEPIGTVSVNFKTEFE
                     SSGINQTRHRIYLEAQTQVKVVIPLITSTKQIKAQIPICETIIVGDVPESYVNIPEKN
                     LGNVLPNTGKNTNK"
     gene            complement(11319..12326)
                     /locus_tag="PC471_000461"
     CDS             complement(11319..12326)
                     /locus_tag="PC471_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase S55"
                     /protein_id="HDF2787602.1"
                     /translation="MQLKNFHFKNNTLSKKTNLAKSKFTIVLTFLFLLYFFSNNLIYA
                     QNLQKNEPEYLIPIGNVLQIDAELKNIIVRNPGEASPFRLGDAIVKVNNAEVDGYSDF
                     ANTLNALPEEKQVSILLNRGGQLITMKTTKHILEKISFNNLLSGFATLTYINPDTSEF
                     GAVGHPISVGNARKIPIKTGSISTTKHLNIEKSYKGSVGCINAKRENIIGSFTENTDF
                     GIRGQINNFDISKLKKYKVASLDEVKLGKAQIILQDNFNKCKKYNIEIIDIENQKHPE
                     SKSFKIKITDKKLLAQTGGIVQGMSGTPIVQDNKIIGAVSHAIENDPAVGYGVFIKWM
                     L"
     gene            12554..13438
                     /locus_tag="PC471_000462"
     CDS             12554..13438
                     /locus_tag="PC471_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HDF2787603.1"
                     /translation="MKKYRLLVTCIIAMGIIIIGTSNPVSNFYKTTNLISSGNITNEK
                     ADKDENSNKNNKDKNEDKSNSKSNKDKSSEDKSSDNKNEIQKNKKLLICIDPGHQGKG
                     DSNLEPVAPGSSSKKARVSSGTEGIATKKPEYVLNLEASLVLKSILESKGYNVIMTRE
                     THDVNISNSERAILANDKKADMVVRIHADSLNNSSKTGASILIPEKDGKYTAPIYEES
                     NKCAEFIKQNMEQSGIQINGIVQRGDLTGFNWSKVPAVLVEMGFMSNYNEDQMMSNPD
                     YQRKMMQCIADGLDAYFK"
     gene            13640..15190
                     /locus_tag="PC471_000463"
     CDS             13640..15190
                     /locus_tag="PC471_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018592108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HDF2787604.1"
                     /translation="MLVVENVSHGFGARTILENVSFRLRKGEHIALVGANGEGKSSFL
                     NIITKKLMPDAGNIKWSSRATVGYLDQHTVLSKGKTIREVLREAFKHMFDLEQEMIAM
                     YDKMGEASDDEMSKLLEETAEIQTILENSGFYMIDAKIQEVANGLGLGEIGLDKDVTD
                     LSGGQRTKVLLTKLLLENPTILILDEPTNYLDEEHITWLTKYLQEYENSFVLVSHDIE
                     FINNTCNVIYHMENGELNRYKGNYDEFVRLNDIKKRQEEQAYDKQVEERKRLEDFVAR
                     NKARVATRGMANSRQKQLDKMEILERPKEKIKPTFAFKDARAASKIIFETENLVLGYD
                     EALTKPLNFHLERGKKIALKGMNGIGKSTLLKTLLGIIKPFEGNVKLGDYLEVGYFEQ
                     ESSRENSNTPMDEVWSEFPGLTNFEVRQALAKCGLTNEHITSQMRVLSGGEAAKVRLC
                     KVMLKNINFLVLDEPTNHLDVEAKDELKKAIKEFKGTVLLVCHEPEFYSEIVDDVWNI
                     EDFTTKIV"
     gene            15219..15866
                     /locus_tag="PC471_000464"
     CDS             15219..15866
                     /locus_tag="PC471_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HDF2787605.1"
                     /translation="MKKFLNVLFSICISIVIIVGVINFTVGFKQLYYFDIDYLNISEL
                     SGLSKDDIKLNYDYLIDYNLNKNVSEFKLPTLKSSPQGKIHFEEVRNIFQNINKLAKL
                     LLVVSLVGIILSVKNKNIKILKTTSITLIIMPLLLTVPILLNFEKSFIIFHKLLFRND
                     YWIFNPDLDPVINILPEEFFFHSGMMILILILLVSIVLFVMYKLCKSLSIKIFQC"
     gene            complement(16008..16268)
                     /locus_tag="PC471_000465"
     CDS             complement(16008..16268)
                     /locus_tag="PC471_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich small domain-containing protein"
                     /protein_id="HDF2787606.1"
                     /translation="MGENYKFFNHKDCEFFPCHKTNKPEEFNCLFCYCPLYALGENCG
                     GNFRYTDKGIKDCSSCILPHKKDNYNYIMSKFQDLVKITSKK"
     gene            16485..17768
                     /gene="hflX"
                     /locus_tag="PC471_000466"
     CDS             16485..17768
                     /gene="hflX"
                     /locus_tag="PC471_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HDF2787607.1"
                     /translation="MKEIQEKALLVGLNLTTIVKKNDDIDTNESMEELKELTKAAGAE
                     VVGSLIQNKHSVDAAYYIGKGKVEEIRAYSDSLDATLVIFNDELSGAQIRNIENVVGR
                     KVIDRTTLILDIFAQRALSKEGKLQVELAQLKYRLPRLYGMGGEMSRTGAGIGTRGPG
                     EQKLEIDKRHILNKAADIRRELKEVKKNRETQRVKRLKSNIPIVALVGYTNAGKSTLL
                     NELIKTHKDYEQEKEVFVKDMLFATLDVTLRKALLPNKKEFLVVDTVGFVSKLPHDLV
                     EAFKATLEEVQYANLILHVIDATNTSYELQKSTTEGVLKELGVNDKKHILVYNKVDKL
                     ELDIYPKSQEDIVYISAKQGINMDKLLNMIEIALMENTYSVSLMLPYERGDIFSRIKD
                     KYNVENFEYGESGITLDADLDEEDFNIYREYILEK"
     gene            17776..18453
                     /locus_tag="PC471_000467"
     CDS             17776..18453
                     /locus_tag="PC471_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HDF2787608.1"
                     /translation="MDINWENIDNLEDYFITYLLYKESKTVSQISKIRNISSTEASEQ
                     LIQAKLKIKEMQKDDFEASKDILDKFLELDKIKRLDFMDSLDDEKMVYFKRKVFKRIL
                     VEKNAEDLIVLIWATGELKDDRFLKLLHQLTNHRHSDIRRITYSAIRKIESPSSREVL
                     QKGLYDKNAQTRQYCAKALSKLGDENSLKILQQLKDKNKNFEKEYVLRAYDEAIKSLE
                     NAKNLKK"
     gene            18539..18982
                     /locus_tag="PC471_000468"
     CDS             18539..18982
                     /locus_tag="PC471_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HDF2787609.1"
                     /translation="MIVRRCAGGVVFYANKVLIVKNDRGEWTLPKGKILGGGLPYESA
                     VQRVKVETGIDAKMIDVAGDTMYEFFSRSRQQEVCNAIMWYVMEACNTDCVLAPEFQE
                     GGFYKVKDALEMLSHHKEQALVEVSYKKFKELKKLVSEGDDVPAN"
     gene            19287..19931
                     /locus_tag="PC471_000469"
     CDS             19287..19931
                     /locus_tag="PC471_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HDF2787610.1"
                     /translation="MSNYRTLHEFGTDEITIEKSVFIGYAKPIKSEEEALEFINEIKK
                     KHKDATHNVWAYTVGKNMNIQRYSDDGEPQGTAGIPTLEVIKKEDLRDVAVVVTRYFG
                     GTKLGAGGLVRAYTKGAKLGLEAGKIIYKVMYQEVKVKIDYTQLGKVQNELMNLGYFI
                     KDTVYEDNVEIVVYSRLEDVEKLSEKMIDITSGTGKIVLGEEFYLSEQNGEILI"
     gene            complement(20017..20394)
                     /locus_tag="PC471_000470"
     CDS             complement(20017..20394)
                     /locus_tag="PC471_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HDF2787611.1"
                     /translation="MLFALIGFFAGIIGGMGMGGGTILIPALVLLAGIDTKIAQSVNL
                     LSSIPMTLIALAIHIKNKNVIFKLVIPIAVFGVLGAVFGSLLANYLSSDILKKIFGIF
                     LLLVGLFEVKKGFCAKSDKKPLN"
     gene            complement(20394..20780)
                     /locus_tag="PC471_000471"
     CDS             complement(20394..20780)
                     /locus_tag="PC471_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HDF2787612.1"
                     /translation="MKKTDDLKKKYFDFNLKNTIIGVFTGFINGVFGSGGGTLLVPIL
                     NDIVKVEEHKSHATALSIIIFLTTASSVLYVSKGTYDVNLTIKVAVGSILGGIIGAKL
                     LNKVTGKFLRISFGIIMIIAALRMVF"
     gene            20959..21714
                     /gene="nadE"
                     /locus_tag="PC471_000472"
     CDS             20959..21714
                     /gene="nadE"
                     /locus_tag="PC471_000472"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HDF2787613.1"
                     /translation="MSNIKIQIDKTVEWLINKVNEANAKGLIVGVSGGIDSAVVANLI
                     KKAFPENSMGVIMSIKSNPQDREDALKVIEGCDIEYLDLDLIEPQSAILDMVVGNLKD
                     KHLYREEYLKMTDANLRARVRMSTIYTIANNLGYLVVGTDNAAEIHTGYFTKFGDGGV
                     DILPIANLTKGEVYEWAKELGVHEDLINKAPSAGLWEGQTDEDEMGTTYNMIDAVLEG
                     RLDEVPKRDQEIIERLHRLSEHKRKTPAQPPKF"
     gene            21797..22516
                     /locus_tag="PC471_000473"
     CDS             21797..22516
                     /locus_tag="PC471_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDF2787614.1"
                     /translation="MGRIGNIINRKGKQDAQRAKIFTKHARAIAVAAKEGGADPEYNA
                     ALKTAIEKAKADNMPNDNIDRAIAKGAGAGAGEDYETIVYEGYGPGGVAVIVETLTDN
                     KNRTAGNVRYYFDKNGGNLGTSGCVSFMFDKKGQILVGLGDGVSEEELMDVALEAGAE
                     DFITEEDGYEIITTPEDFSSVRDELKAKGYEFISADVKMIPQTTTVLTEESHLKMMNK
                     LVDMLEEDDDVQDIYHNWEVE"
     gene            complement(22683..23687)
                     /locus_tag="PC471_000474"
     CDS             complement(22683..23687)
                     /locus_tag="PC471_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HDF2787615.1"
                     /translation="MATIKDIAKDCGLSSATVSYVLSGKGNERRIPLHTQELILNSAD
                     KLGYKRSTPIKHNNIKIAFYWQDEGFELASTNILQAINHIISLEHLNINIIIRPYKVG
                     HLCDDEYFFANNYYDGIVLTGINADDIEYLVKNKPKIPVVVVNREIPGFSSVSIDNEE
                     TGRIAALHAIQNGKNDIAVVFNPTSFTCMTLRGKSFLKTCKEHGIHLSDSIYYCSNKI
                     DSGYDIAQKMLAKNSLKKVIFCNHDVVALGMMNAFHESGIVIGEDIQIFAASNGPSRL
                     MARSIPPLTVIDLKMEETAERSLRMIIDTVTGRINGIQQQVIYPSIIYRQSSPAQNLL
                     "
     gene            23953..24849
                     /locus_tag="PC471_000475"
     CDS             23953..24849
                     /locus_tag="PC471_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HDF2787616.1"
                     /translation="MSKVNIFEVGPRDGFQNLKEFLETDKKLKIIDGLIASGIKQLEI
                     SSFVSPKAIPQMKDAKEVATYCVEKYPDVKLYALVPNLYGAKVAWECGIRDISYVISL
                     SETHNKKNINRTHEESIDELKKIMNTYPDMNICIGMATVFGCPFEGLPKIEDAIRFAK
                     RLWENGIREINLADTIGIADPKQVRETISALVEALPECRWQVHIHDTRNMGMVNTLVA
                     IESGITTVQSTLGGLGGCPFAPGASGNTATEDLVYMLERMGYDTGVDFVKLLELSKYQ
                     KSFVNGVYSGHHVNIKSDSCNC"
     gene            24970..26232
                     /locus_tag="PC471_000476"
     CDS             24970..26232
                     /locus_tag="PC471_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2787617.1"
                     /translation="MDIIALLLFLGVIIIAFVRKNNAGILALAIGVIAVRIFGMDDKE
                     LYGAISNSMFTTLVGITLLFAIVNSTGALKLVAEKIVAASGKRVWLIPIAVFIAGFVV
                     AGVGPGAIPALAIIPAMAVPVAITVGYNPVMLALIGECGLMAGRMTPITPEGQIITTV
                     AEGVGIENVMPTILACQTLSTIVFSIVLFIIFKGYKLKKPINVIDSKQIEKFNIKQII
                     SLISIVVMIAFIIIFDMNVGLAAFAVSAVLIFFNIADEGECIKSIPWSTIVMVLGVGA
                     MMTIVDAAGGIDLMSNALSSLMNSKTATPIMSISAGLMSMVSSALAVVYPTMMPMCVD
                     IAKAVGGVDPLALIAAVGVGGSLAGVSPLSTGGALILAAMGSSKKDFNKEEQNKVFVQ
                     LFIMSAVGLLVIAVVSILSFNAITHLMN"
     gene            26308..27501
                     /locus_tag="PC471_000477"
     CDS             26308..27501
                     /locus_tag="PC471_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HDF2787618.1"
                     /translation="MKKGALSNIKVLDLTRVLAGPYCTMMLADFGADVIKIEMPRKGD
                     DTRGFGPFVNGESLYYANINRNKKGITLNLKSEEGKNIFLEMVKHADVVVENYRPGVM
                     EKLGLGYDELAKVNNQIIYGAVSGFGCYGPYSERPGYDIIAQAMGGLMSITGQEGGEP
                     TRVGNAMGDVLGGMNLTIGILTALNARKIIGKGQRVDVSLVDSVVSSLETGIQRYFDS
                     GQPELMGNRYASAYPYDSFKASDGSFVIGCGNDKLFEKLCKKVLFREDLLIDERFKTN
                     LNRCEHYKALKPEIEKWSVKHTIKEAVDLISEAGVPAAPIYNLDCVTTDEHIAKAREM
                     IVDISHPIIGNMKVNGNPVKLMTTKAKIDMPAPSLGESNEEIYNGWLGFDSDKIKDLK
                     ENGVI"
     gene            28130..28924
                     /locus_tag="PC471_000478"
     CDS             28130..28924
                     /locus_tag="PC471_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HDF2787619.1"
                     /translation="MESVMENLNNLKVEVKDKVCVITINRPKALNALNSDTLRELSQV
                     IDVVSENEAILGVIITGEGKVFVAGADIRQMQNYKSEEGRKYAGYAQGIFDKIEALEK
                     TVIAAVNGYALGGGCELAMSCDIRIASEKAIFGQPEVNLGVIPCFGGTQRLSRLVGTG
                     IAKELIFTGRQVNAEEAKSIGLINKVVPSDLLLEESMKMMNQIVEKAPIAIRYAKVVI
                     NKGIDMDLKNALELEKDVAGLTFATRDKQEGMNAFIEKRKPVFENK"
     gene            29019..30359
                     /locus_tag="PC471_000479"
     CDS             29019..30359
                     /locus_tag="PC471_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HDF2787620.1"
                     /translation="MNTGVIISLVGIIIALSLLVVMVMKGINIFIIAIVCSSLVAITG
                     SLNVYDALKVDYMTGFVGFLQANFFIFLTGTLMGKIMEITGGAKSIAKMIVRWIGKDK
                     ALLSIPIACGILAYGGVSVFVVSFAVFPIALEIFKEADLPRRFIPAALTFGCSTFAMV
                     APGAPQIQNIVPASTLGTDIMAGAVNGFISCAVMFIVGSTILYKMVSKEKANGGHFIA
                     HESDIFDEAEATNSKRENGPNGVIALIPLIVSILIINVKVNGKAIVPIEVGVFIGALL
                     VYLLLNKYQDNTKIVGHVGDACKTTVVAICNTCAVVAFGSVVKSAVGFDFIVNAMTNI
                     PGPPIAGAAVGTTVIAGICGSASGGLGIAAPLLGPVFLAKGVSAAALTRTMAISSAAL
                     DSLPHNGYIVTVTNGLCKETHKDAYMPIFWVTVLTPFIGTIVAVLLFTMFPGLP"
     gene            30496..31728
                     /locus_tag="PC471_000480"
     CDS             30496..31728
                     /locus_tag="PC471_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HDF2787621.1"
                     /translation="MLNKPLEGVKVIDLTYFVAGPGTSKILADWGADVIKVEPSFGDP
                     GRKTGATMTMPIDDYNNPFYSTYNSNKRGLSINLKSETGIEIMDKLLSEANVFVSSYR
                     TGALKRLGLDYESLSKKHPHLIWGQINGFGDFGPAKDNAGFDTVAFWARSGAMLDIAE
                     KDTSPINPPIAFGDATTSCSLSGGICAALYQQAKTGKGQKVMASLFGQAIWNASSLLA
                     STQFSDEYPKTRKNAISPVINSYRCKDGKWIFLSILEHERYFEALCRLFGREDLIKEE
                     KFASSVESKKNATELINILDGEFAKFTQNEMVEKLTAADIAHEKIQHVNDVVTDEQAI
                     ANNYIYEHTSKDGNKTMLASTPVKFGNIEVNMTCDAPLIGEHSDEILRELGYSDSDIE
                     TLIESGIVTMNKEACEIC"
     gene            31768..32910
                     /locus_tag="PC471_000481"
     CDS             31768..32910
                     /locus_tag="PC471_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HDF2787622.1"
                     /translation="MDFRLTEAQLMLQRVAKEFAENEIAPIAAETDKTGIFPRELFSK
                     MAKIGFNGIGTPVEYSGSGGADIEKVIVVTEIAKKCAASAAILSIHTIYAQAILKFGT
                     EEQKKKYLPMMAEGGCVGAFALTEPNAGSDAARAATTAIIDEETDEYVLNGTKCFISG
                     GGQAESLIIFALTDPSKGIKGMSAIIVDKGTPGFSIGKIEEKMGIHGSETAELIFDNC
                     RVPKSNLLGKEGKGFNIAMTCLDGARIGVGAQAVGIAEGALEESIKYSKERVQFGKPI
                     SALQGIQWYIADMATMVESAKLLVYYAADLKARGEKHTKEAAMAKYNASRTAREVTNL
                     ALQIHGGYGYMKDYPLERMYRDAKITEIYEGTSEIHKVVISRAVLG"
     gene            32921..33715
                     /locus_tag="PC471_000482"
     CDS             32921..33715
                     /locus_tag="PC471_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009899720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HDF2787623.1"
                     /translation="MNIVVCLKQVPDTNEVKINKETGTLIRDGVPSIINPDDRNALEE
                     ALKMKDELGAVIKVISMGPPQAKSALKEALAMGADEAYLISDRAFGGSDTWATSTIIA
                     AAIEKVGKYDVIFCGRQAIDGDTAQVGPEVAEFLGIPQVTYAKEVKVQDDKLLVTRYT
                     ETGDYLIEAKMPVLLTAIKELNNPRYPSVKGILEAYNNGDAKITVLTLADLDVDTTQI
                     GLKGSPTNVYKSFVPVKDKHNEIIEGINKKEKAEKLIEILFDLKLV"
     gene            33726..34748
                     /locus_tag="PC471_000483"
     CDS             33726..34748
                     /locus_tag="PC471_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HDF2787624.1"
                     /translation="MMRAKVNQGINLNDYNGVWVIGEQREGKINPVTIELIGEGRKLA
                     DQLGKELAVVIAGYEVEKEVKELLHYSVDKVYYINDPLLKDFTTDGYAISIANLIERK
                     KPEVVLVGATSIGRDIAPRIAGKVGTGLTADCTKLEIDSTDNKLLQTRPAFGGNLMAT
                     IVCPKNRPQMSTVRPGVMAKAVRNESETGILEVVTPELTEKMIRTRLVEILPQEKKSV
                     NLTDARIIVSGGRGLKRAEGFELIKELADKLGAEIGASRAAVDSGWIEHSHQVGQTGT
                     TVRPELYIACGISGAIQHLAGMSDSKYIVAINKDAKAPIFSICDYGIVGDLYEIIPEM
                     IESLNR"
     gene            34847..36223
                     /locus_tag="PC471_000484"
     CDS             34847..36223
                     /locus_tag="PC471_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HDF2787625.1"
                     /translation="MKEQWYKDIFARVLSMTDDGFIVVNTSGVIIDINDKYCDFLGKE
                     RKDIIGQNIQSIIPNTKMLDVMKNKYCEEGAIHHYSGGNTKEKSVIVSRSYVENDNGE
                     VVAGVAQVKFRLQSFDVAKKLMSEYMELQYYKEQFKDNCGFDKLIGENRDFIELKKTG
                     VKASKTNFPVLLTGETGTGKEVFARAIHNNSSRSDKPMVSINCAAIPEELLESELFGY
                     DEGAFTGAKKGGKKGKFLVANNGTIFLDEIGDMPLTMQAKLLRVLQESEIEPVGGLKT
                     IKIDVRVISATRKNLSKMVEEGLFREDLYYRLNVINIHMMELKDRQDDILLLANYILN
                     KLNVEYKELKVLSDKVKNCFINYTWPGNIRELQNVIKSAYAVSDDMVIMMCDLPSKMD
                     NISRVAQCNVDSNCSIHEMVENYEKSLIIDVLRKYNWKCSKAAEVMGIHKSLLYKKIK
                     KYEIELNN"
     gene            complement(36555..36899)
                     /locus_tag="PC471_000485"
     CDS             complement(36555..36899)
                     /locus_tag="PC471_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2787626.1"
                     /translation="MDQIKRLHELQQKSYLTKEEFEELKNNTYIERFELRDECDNSYI
                     YTFYTNDKCNDIKFIKSQFIVTLIKIGFLCECGGVFRQKKIVDEYDYGYNAIYKCDSC
                     GKELDKEVENYD"
     gene            37387..38397
                     /locus_tag="PC471_000486"
     CDS             37387..38397
                     /locus_tag="PC471_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4116 domain-containing protein"
                     /protein_id="HDF2787627.1"
                     /translation="MHEDKNDIFESIDTEDFDNKTEEECLELVRKNGLNLIYIKNQTE
                     EICLEAVRQNCNAIKCVQNQTEKICIEAVKQDWRMLEFVEEPTEEICMLAINQDGTAL
                     KYIENQTEELCLRAVEKNGAALEYVKEQTEEICIEAVRNSEFGLARVKNQTEKICMEA
                     VKHCSYNLKWVENQTEEICMEAVRQNGLDLKFVKNQTETICLRAVRQNGMALEFVKEQ
                     TVGICLKAVRQYGMALKFVKEQTEEICTEAIKQDKRALSFVKGDKEKYKALYDNNEPF
                     AKRYVRNVIEKENRALIKKGEENAKIKIAGKLYDRGMSFEDISDIVEIEISKLKVSLG
                     VF"
     gene            38533..39021
                     /locus_tag="PC471_000487"
     CDS             38533..39021
                     /locus_tag="PC471_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HDF2787628.1"
                     /translation="MKITVLYQTRSGNTERVAKLIEEGAKKVDGVDVKLMRLDNIDLD
                     YLNESKAVIFGTPTYLANTTWEVKKWFDEDSKKVNLAGKLGAVFATCDYICGGPDVAI
                     LTIVGHLMVKGMLVYSGGGSLGKPFIHLGHVHSIEGPELQDEKAIIFGERIATKAKEL
                     FA"
     gene            39197..41125
                     /locus_tag="PC471_000488"
     CDS             39197..41125
                     /locus_tag="PC471_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HDF2787629.1"
                     /translation="MVWHIEYEIFSAIIVIFLMVYFFRSKFIPTLQNKIYCALLIFSF
                     LFIISNILGSFCLNNIDKIPIFITFLLNQIYLLLLPIPAALVSFYVMAIIYQDIRYMK
                     KRLLFLSIPLIICIILSITNPFTHILFGLSKETGYIRGCGYVATFASFFFYAIYSAFL
                     SFKYRKAMHESKIWAIRGFLIVSIVAIILQAIFLQYLLTGTACACSLLLTYLSMQNRG
                     LIIDDLTGVLNRQSFIQELDLNINTEQSGFIITIALDDFKFMNETFGTKNGDIALKEV
                     GKYLIQIHDVNHVYRIGGDIFSIVVNSQLGIEPDDIIRKIEERFKKPWLVEEISFNLA
                     TSIAVVYYPENAETTEDVIVAIDFSIHEAKRSESRRVVYADASISEKIKKKHVIKNCL
                     KEALKNDGFEVYYQPIFSNSRGRFTSAEALLRLEHKELGFIPPDEFIPIAEQTGLINS
                     IGLVVFEKVCRFIASDEFEDLKLDNIAVNLSVVQCMQKNLADDLLYLMNKYNVSPTKF
                     KLEITETVAMGSFNIIKETMERLIDLGVKFALDDFGIAYSGVTNMLSLPFSVIKLDKS
                     LIWSMNEDSRHKLTVETIIALINKLNMKAVAEGVETIKCAEDLIAMECEYLQGYYFSK
                     PVPEDVFKNLLKENTFTS"
     gene            41302..41712
                     /locus_tag="PC471_000489"
     CDS             41302..41712
                     /locus_tag="PC471_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HDF2787630.1"
                     /translation="MNKKKILVPIDGTERSMHSLEFIKGIFKKDEVEVEIMNVKELVF
                     IDGISLAEEIKNSENLGRRILDKAAEIMGEYDVKVHFTFGYPGDEIIRKAKEDNVDFI
                     VMTKSTKKGLTRMIGSVTASVVKQAKCIVMIVPE"
     gene            41734..42036
                     /locus_tag="PC471_000490"
     CDS             41734..42036
                     /locus_tag="PC471_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIBC"
                     /protein_id="HDF2787631.1"
                     /translation="MNKKIVAVCESERSLNSLKTAANDLGFDLVYEIQENDNIKNELS
                     IYDIEDASIVLFVTSDSIESIEKIDRFIDREFYEVDPKYIIEDAKSIVSEILIDLN"
     gene            complement(42253..42945)
                     /locus_tag="PC471_000491"
     CDS             complement(42253..42945)
                     /locus_tag="PC471_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HDF2787632.1"
                     /translation="MKSLNATEKIIDYIKLNILNGTFKINSKLPSERKIADLFNISRI
                     PVRNAINILCKEGILRAVPYSSPIVEGFKKVDLFDDGEIYKNHNIQEFYVESLRARQL
                     IESEATKLAILNATSKELQKIKYTYLKSIEELDKVSHGLIEECYDADLQFHKEIILAS
                     HNPIFIKYYELIPKTVSSNQYFGFKYRNSLKDMISHHNKIMIAFDSKDSYLGYTSMYN
                     HLEDVIQLFQHD"
     gene            complement(42971..44158)
                     /locus_tag="PC471_000492"
     CDS             complement(42971..44158)
                     /locus_tag="PC471_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDF2787633.1"
                     /translation="MKKISNQKLLLLSIVTFFWFSQYVYIPFQNPYLSACNVSTNMIG
                     IIIGAYGISQCLLRLPVGVFADSINKHKIFILSGIFFAGLASLIRVFSPGGNGFLIAN
                     ILSGISSSTWISYMVHYTNFFSTEHQQKATSTIILVNNLGMLIGFVMSTLFYEKLGMT
                     ALCIMSVLSSVIGTILALNIKNHNSKKIPEKISIKKYLSICKDKRLVFFSFMALIQQG
                     IQMSTTMSFTTQILKNLGSSSTLIGLSSIFYMVCAVASSGFASTKFCTKYGPKFWIPL
                     VFILVSAYCFLVPTIKNINIIFVLQVLPGISTGILLSYLTSESMKNVPKHQKSTAMGF
                     FQAIYAIGMSVFPMIVGKISEYTSIQYGYFILAIIAILASILSYTYYKKVDLTLNYDI
                     IQS"
     gene            44494..46371
                     /locus_tag="PC471_000493"
     CDS             44494..46371
                     /locus_tag="PC471_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDF2787634.1"
                     /translation="MDYKNIAQEILLNVGGKENVNEVTHCMTRLRFKVKSASKVNKDK
                     LSKTEGVITVVESMGQIQVVIGNKVKKVYDEVIKIVPQSNNIGKKNESQEKQGIINSI
                     LSAVAGIFTPTIPAIAGVGMIKGILSVLAMYYMNKNGVDIKETQSYIILNAMADSIFY
                     FMPIILGYTAAKVFNANKIISMVLGATLCYPTFTALMAGEESVRFLGLAVTKATYTSS
                     VIPIIIAIWALSYVEKVLEKYIPEVIKIIMVPTLSLVVMLPATLFLFGPIGIYIGNVI
                     NFSYKYIYELSPALCGAFVGGLWCVLVIFGAHRALLPIGISDVAQTGRQNLLAFAGAA
                     NFSQAGAALGVFFKTKNQGLKTISMSATITALFGITEPAIYGANLRLKKPMVCAVICG
                     AFGGAIMGMGGAYGNAFANQGVLTIPVYAEAGALGFLSYLGGCAIAFFGSAISTYLVG
                     FEDLEESSNKENSSIKVETKDGVIDITSPVEGECIELSEVKDDVFASKAMGEGIAVLP
                     TKGVITAPTDCEVASLFPTLHAIGLKLDNGAEMLIHVGINTVELNGKYFTKHVNQGDL
                     VKKGDKLISFDIDKIKKAGYDVTTPVIVNNTFDFGQVVSCKSSYVSTNDNIISLVLAG
                     N"
     gene            46728..48134
                     /locus_tag="PC471_000494"
     CDS             46728..48134
                     /locus_tag="PC471_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HDF2787635.1"
                     /translation="MNTGFPKDFLWGASSSAFQVEGAWDKDNKGKTVADYNSFKKSHL
                     QADTKVASDFYHNYEEDIELMKELGMKTYRFSISWARIIPDGEGEINQKGLEFYNKII
                     DKLIECDIEPFVTLYHFDLPFKLVEKYNGWESRETVYAFERFAKICFKHFGDRVKYWQ
                     PHNEQNLIVRVEERINIYDETDSWKIDKMRAQMDYNLCLAHALAVNACHEMIKESKIG
                     AAVSSSVTYPLTSKPEDVYAARMNDNFKVYYMLDMHHYGEYPGYYMKYLEKRNIVPHM
                     EDGDKEILKKAKMDFIAVNYYRTNCAEALPEDSQHPFGLREGTVDFSMYGLFKMSMNP
                     NLEASEYGAAIDPSGLRVALNEYWQRYHLPVIITENGLGAKDILEDGKIHDDYRIDYL
                     RSHINACKLAIEDGVEMIGYCPWSFTDLLSSSQGFNKRYGLVYINRTDHEVLDLKRIK
                     KDSFYWYKEVIENNGIVK"
     gene            48162..49010
                     /locus_tag="PC471_000495"
     CDS             48162..49010
                     /locus_tag="PC471_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDF2787636.1"
                     /translation="MYITKVLNNSLLLAKDDNDEEIILMGKGIGHNYKAGSELNKKDI
                     EKIFVLHDENIKKSIIQLATEIDAEYFGIAQMIIAYGIEKYNLKLMNHIYLALTDHIA
                     FAVRRFKEGIMIENHYLFEIKEFNPKEYDIGKYAITVFKEVLGLDLPEEEIGNIAGHF
                     INAQQDNPYSDRNKRSAKIVNAILQIVHYHFSIVYNKESFYYRRFVMHLKAFSQRFLA
                     NEPSREKIDFIYEQVQKNCKEEYECVKKVGAYMYKEYSRELPRQEELYLMIHIHKILG
                     ELCENS"
     gene            49671..50363
                     /locus_tag="PC471_000496"
     CDS             49671..50363
                     /locus_tag="PC471_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003020373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2787637.1"
                     /translation="MNKVLIIDDDKDLCILLKKSISIEQIEANYCHSGRDGLVELSKQ
                     DYQLVVLDVMMPGMDGFETMEQIRKYSSIPILMLTSKSDNYSKVHGLRAGADDYLTKP
                     FEITEFTARVLSLIRRYTRFNPKNEEPQQLSFKGLLIDLDQHTVTTNGEPMLLPPKEF
                     GVLLYCARHQGKILTKQQIYEAVWGEPYVYDDSNIMAIISRLRKKIELDPSSPIYLQT
                     IKGIGYQFNTEV"
     gene            50360..51295
                     /locus_tag="PC471_000497"
     CDS             50360..51295
                     /locus_tag="PC471_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF2787638.1"
                     /translation="MKMDEQTIILFLLLLSIGLLFLSIFAFSKLGHIYKRLKDIEEIL
                     ADVQIGDENRKILIKPCDVMAPLVYQLNEIVYDYENKLLSLKKSDKASKQLMTSLSHD
                     VRTPLTTLIGYLDAVHSGIVIEQEREEYLEIARRRAYDLKDYIDVLFDWFRLNSDEFT
                     LSIESVEIAELSRNILKDWIPIFHEKKLNFEIDIPENRLMVNLDPDGYSRVVNNLVQN
                     VLAHSKARQIKITMSEDSRMVLLRVEDDGVGIARENLPHIFERLYKCDKGRSEKGSGL
                     GLSIVSQMVERMGGQVSVESEIGKYTVFTVSLPLI"
     gene            51443..52366
                     /locus_tag="PC471_000498"
     CDS             51443..52366
                     /locus_tag="PC471_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013273760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2787639.1"
                     /translation="MNNLIIETKNLTKQYGAQKSVSNLNIHVQKGRIYGLLGRNGAGK
                     TTTMKMLLGLTKPTTGEIKIFGQDIHNNEKKLLPRIGCLIESPGFYPNLTGTENLKIF
                     ARLRGIPKKNAVESALDVVGLPNNDKKLFSQYSLGMKQRLAIALAIMHDPQLLILDEP
                     INGLDPIGIAEVRTFIRDLCTERGKTILISSHILSEIALLADDIGIIDHGILLEEESL
                     AQLEEKNSKYIHFAVSDAAQAARLLETQLNLKNFKVADNYNIMLYDTDVSVAEINRAF
                     ILNNIEVSESHLCEDTLEDYFKKVTGGEGIA"
     gene            52350..53120
                     /locus_tag="PC471_000499"
     CDS             52350..53120
                     /locus_tag="PC471_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2787640.1"
                     /translation="MKELLDIISCEFSKLKRLKFILISILGACLFPIPATILIAKDNL
                     PFEQLFKLVVNFGYFLLLPIVLSIVASQLFFIERDNDVLKNLATVPVQKGKLALAKLA
                     VLLFISLFYSVAGLGATIIGGFIVGIVEGVAVKLGMSIAFGIMFFVTVLPVVVLIVYF
                     NRSYIFSIILSFVYAIFNFSISLNIINFEPNNPILSVLPAPVIMRWWMAFWGDPTGEY
                     TALRQPYLLSTPACVGILCLITIIAVLLICMIYKKQEN"
     gene            53130..53912
                     /locus_tag="PC471_000500"
     CDS             53130..53912
                     /locus_tag="PC471_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2787641.1"
                     /translation="MLDIITTEFQKIKRYNILWIGVVAVLFSALLAVFQVVSSHGSDP
                     LTYESFANGVIWNNFSLSFPFGITLIGGYLINREYTDQTLKNILTVPISLRKLLVGKL
                     IAVGGITVLLSLFSFLCTFILGLLFCNINISVTLVVKSLVQILSVNLYCYVAVLPIIA
                     YFGRKQNAFLTGVGIAFVYGFCGVFVAGRNLTDFYPITAGLGLVGYNNGAGTVYQPLI
                     GAMTLIVILILTTILLVFTPNYDKVMTISKKKGIKHKKYSHK"
     gene            54289..54825
                     /locus_tag="PC471_000501"
     CDS             54289..54825
                     /locus_tag="PC471_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HDF2787642.1"
                     /translation="MNLKGTKTEKNLMAGFAGESEARNKYTYYASKARKEGYNQIAAI
                     FEETANNEKEHAKLWFKLLHDGMPSTEENLKDAAAGENYEWTDMYAKFAKEAREEGFD
                     KIAYLFEAVGKIEKEHEERYLKLLENLNEGKIFKRDEEVVWQCQNCGHVYVGTEAPEK
                     CPVCDHPKAYFNIKAENY"
     gene            54857..55246
                     /locus_tag="PC471_000502"
     CDS             54857..55246
                     /locus_tag="PC471_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HDF2787643.1"
                     /translation="MKFSKQRELILNEILNNPVHPTADYLYENLKKDNPNLSLGTVYR
                     NLAQLTEHGFIRKVSIPGYPDRFDGRIDNHYHIICEVCGEVYDLESEVLNNLQELISE
                     ETDIKITSYNISFKGICNNCKRCSQVG"
     gene            55283..55669
                     /locus_tag="PC471_000503"
     CDS             55283..55669
                     /locus_tag="PC471_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="desulfoferrodoxin"
                     /protein_id="HDF2787644.1"
                     /translation="MCSEQKFFICETCGNLVGMIQSGGVPIFCCGKPMKELVPNTTDA
                     AVEKHVPVIEVDGNNVTVKVSSTTHPMTKEHHIAWVYLMTEQGGQRKCLAVDGEPVVK
                     FALNDDDKVISAYAYCNLHGLWKAEL"
     gene            55796..57238
                     /locus_tag="PC471_000504"
     CDS             55796..57238
                     /locus_tag="PC471_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HDF2787645.1"
                     /translation="MSIYKCSVCGYIYDESKNDKTWDELSEDWECPVCTKGRSYFGKI
                     STVYYEEDEKIAEDIVEDESKLNTEKEGDLNYLSTYLRRDDEVEKHMDIIHEMAVTGK
                     SIIEPMRTKLPVISWDDILIMGAQLNPLPLNEHDEVNTTTIIGKKAKKPMIIENPVYI
                     SHMSFGALSKELKIALAKGAAQNKTAMCSGEGGILPEEKEASYKYIFEYVPNKYSVTE
                     ENLKNSDAIEIKIGQGTKPGMGGHLPGEKVTEEIAKVRNKPVGQDVISPSCFEEIQSK
                     EDLKKLVDELREVSEGRPIGVKISAGHIEKDMEFIAYAKPDFVTIDGRGGATGASPKL
                     LKDATSIPTIFALYRARKYIDTHGLDIDLVITGGLRISTDFAKAIAMGADAVAIASSA
                     LMAAACQQYRICGSGKCPVGVATQDEELRKRLHIENSANRVANFLNVSLEELKTFARI
                     SGHKDIHDLSVDDLYTVNSEISNYTNIQHV"
     gene            57535..58350
                     /locus_tag="PC471_000505"
     CDS             57535..58350
                     /locus_tag="PC471_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDF2787646.1"
                     /translation="MERLKIQVLVDNNTYIDRYFVGEPAVSYYIEIDGNRILFDSGYS
                     DVFISNAEKLNIDLGNLTHVVFSHGHNDHTRGIQFLENRYDLSTVELISHPNCFIPRK
                     HGEKSIGAPFSAEEIKNIFMYNPKDKPFNLSKNCVFLGEIPSINDFEKRAKIGKCKIG
                     DLWEDDYVLDDSAIVCKTDKGIFIVTGCSHSGICNIVEYAKKVCGDDRVIGILGGFHL
                     FELNNRLDSTIQYLDKEEMRMLYPCHCVSFKVKAKMNEILNINEVGVGLTISI"
BASE COUNT        21700 a         6975 c        10695 g        19054 t
ORIGIN      
        1 tttttaaatt attaaaagat attaaaataa taaaaagcat taaaaaagta aaaatataaa
       61 aacaaggtgt ctttatggaa aaatagattt tttctttatt aggcatcttt tttatttttt
      121 gaccaaactt tagatagtca aaataaataa taggtttatt tttttaaaat taacaaataa
      181 tactttagag aggtgtttag tatggataaa aattataaaa aatatgtaga ccaaataagc
      241 ccaaaaccta catatttaaa aaactataca cttgcattta ttgtaggtgg aattatttgt
      301 atgatagggc aagcaataaa tgatttgtat atgaaagttg gaggtcttga taaactaggt
      361 gctagttctg caacatcaat aacattaata tttatagggg catttttaac tggattagga
      421 gtttacgacc taataggaaa aagagctgga gctggttcta taattccaat aacaggtttt
      481 gctaactcaa tagtatctcc agcaatggag tataaaagag aaggctatgt attaggtgta
      541 ggtgcaaact tatttaaaat tgcaggacca gttttagttt atgggatagg ttcttctatt
      601 ttatgtggaa ttatttatta tatttttaag atgttttaga tgatttttat ttaatttaaa
      661 ttttattaat tatgaagaag gtataaaatc tgaaaatagc tactaaagta actaattgaa
      721 aaataattct gcagatttaa ttaatatttt taattaatat taaaaatttg aatagtaaaa
      781 tgcaaaatta aaaaggtgtg aaaatatgaa aaataaaaga attggaaaaa gaacagtcaa
      841 gttagaaaat aaaccaacca taatatcaac tggaactata gtaggtccta aggaaggtga
      901 aggaccacta aaggactatt ttgatatgat tatgactgac gatttatatg gtgaaaagac
      961 ttgggaatta gctgaaagta aaatggtaga gactgcatct caacaagcta tccaaaaggc
     1021 aggtaaaaaa ttgtcagatg taaattatat gctaggtggc gatttaataa atcaaatagt
     1081 tccagcatcc tttgcagcaa gagaactggc tattccattt ttaggtatat atggagcttg
     1141 ttctacaatg gcagaaggtc tttgtatagg ttctatgctt atagatggtg gttttgctga
     1201 tttagtactg tcaggtacat caagtcacta ttgtacagca gaaagacaat ttagattccc
     1261 acttgaactt ggaaatcaaa aaccaatgac agcccagtgg acagttacag gagcaggaag
     1321 tgtattatta gctcctaatg gagacggtcc aaaagtaaag tatgttactg ttggtaaagt
     1381 aatagatgag ggtattgatg atggtaataa tatgggacca gctatggctc cagcagcaat
     1441 agatactatc tactcttact ttgaagatac taatgatgac ccaaatagct ttgatataat
     1501 tgctactgga gatttaggta ctcttggaaa acagatagca gaagactttc taaaagaaaa
     1561 aggtgtagat atatcaaaag tttatactga ctgtggtata gagatgttta accttaaaga
     1621 acaagatgtc cactgtggtg gaagtggatg tgggtgttca gctacagtgt ttgcaggata
     1681 tatatatgat aagttgagaa aaaaagaatt taacaaagta atgcttgtat caacaggagc
     1741 actactttca ccgacaagta cactacaaaa gcagacaata ccatgtgttg cacatgcagt
     1801 agtaatagta aatgagtagg aagtgatata atatgttaat ggactatgta agagtattta
     1861 ttgtaggtgg aattatatgt ttaatagccc aagtaatgat ggattatttc aaaatgcaaa
     1921 ctccatatgt tatggtaacg tatgtaacaa ctggtgtagt tttaactttt ctaggacttt
     1981 atgagccatt agttaagttt gcagcagcag gtgcaacagt tcctataata ggatttggat
     2041 attcacttgc tacaggagta ataaaatcaa ttcaaagtga tggtttttta ggtatttttt
     2101 taggaggtac aactgctaca gctggtggaa tagcagcagc tatattcttt ggatatatta
     2161 tgtcagtagt atttactcca aaagcaaaac cataaatctt tattaaaaga ataataagtt
     2221 tttgaaataa taacttttta taaagcaggt ggatatcttt tagattccac ctgtttttta
     2281 tataaagaag cgtttacata attgaagttg acaattattt acaacatagt gtagtattat
     2341 attatcaaaa taaaatgaag tgaagtaggg gataaagatg gtaactatta aagatattgc
     2401 aaggaatcta ggtatcagtt attcaactgt ttctaggtgc ttaaacaata atcctaatgt
     2461 atctgaaaaa acaaaaaaga aagtggtaga agaggcaaat agactagggt tccattttaa
     2521 tgtaaatgca agaaatttag caaaaaaaga aactaacagg ataggggtaa tcttttcaaa
     2581 taattttaat catcaagata ctagaaagtt ttttagtgat ataatggata gttctataaa
     2641 ttctattgag actaataaat acgattttat aatacagcct aataataaca ttagtggtga
     2701 tagtaatgta tataaaatgg taaatggaca aatggttgac ggattagtaa ttgtgtctca
     2761 atcaataaaa aaagaagagt atgatttttt aaaagacaat aattttccac atgtatttat
     2821 atattttaaa ccatctttta taggagaagt agataatttc ttttgggacg ataacgtgta
     2881 tggaggttat atagctacca agcatttaat tgaccatggt cataaagata taataacagt
     2941 aacttcagat gataagagtt caaaaatgca tgaagataga accaagggat atttgaatgc
     3001 tatggaagaa gcaaatttaa agacagaagt cattaaatct aaaatggatt ttgaatctca
     3061 agttgaattt ttagaaaaat acattgataa aataaaaaaa gcttcagcta tatttgtaca
     3121 acaagatgta ccagcgattt ctataataca agagttaaaa actacttatg gtataaatgt
     3181 accagaagat atttccataa tagggtataa taatattgaa ttaatatcat attttaggtc
     3241 gcatttatca acaatagatg acccaagaga gcaagttata aaaaatggag tagatagttt
     3301 agtaaatata ataaataaaa agagtacaga acacattcca agaaaattat atccaaggtt
     3361 aataatacga aatagcgtta aaagaataaa gtagtaagct tttaagtatc aaacgtttat
     3421 taaaaatatt atagattatg tgaagttttt taacaaataa tataaataaa aattaaaagg
     3481 agtaggtgta tataagtaca ttgtactcct ttatttattg agatagtttt tataatatga
     3541 atgaataatt ttattcaatt atttgaaaat ccgttatttt aacaatataa attaataaat
     3601 tatttgtatt ttatatcatg aatctataat atttttttga ttcataacta aaagtatata
     3661 aaaatgatga ataaatatat attatacatt atcaatgata ggttatataa taaaatcact
     3721 aacaattata ttataagaaa attataaaaa ttacaaggag gtatagaaat gaaagaaaaa
     3781 gcagggatta gaaatgccat aatagcagcc tttataatca caatattggc agaattaatt
     3841 ggacctattt catttaaagt tatgggaatt aatgtaactt tacttagtat actatgggct
     3901 atattcatag gaatggctgt ttctcctcat ttattaggga gagttatacc agccttaaaa
     3961 aagtttatag gggataatga aataaatgta tctccatatt tattatcttt gacactatat
     4021 ccattaggga taatgtttgg aattaatgca gggcctaaag ttggtatatt acttcaagca
     4081 ggaccagcac ttttattaca agaatttggt catatgggta caatgttgat agcattacct
     4141 gttggagtag cattaggtct tggaagaagt gcattaggtg gtactttttc attatgtcgt
     4201 gacacagcac taggaattat tggagataag tatggattaa actcaccaga aggtatagga
     4261 acactaggaa cttacataag tggtagtata tttggaacat tattattttc atttttagca
     4321 ccagtgggtg tacttttagg atttcatcca tatgctttag caatggcatc aggaatgggt
     4381 agtggttcaa tgatgggagc tgcaacagca tcattaatga atacagttcc acaaatgtca
     4441 gagcagattt tagcatattc agctactagt ggattgatta cagctgttac tggaatatat
     4501 atagaattat tcttatcttt accagtagcc aactactatt atagcaaagt agctccagtt
     4561 attgaaagat ttagaggaag aaaaacaaga agaacaagta ataaattgta aaggaggata
     4621 tatatatgga aaaaactttt aagactatta tggagcagat aaaaatagta ttgtttatat
     4681 atattattat ttgctttgga caaatgatag cacttaagac tcaggctcaa gatttgttga
     4741 ttggaagtgt atctggattt ttgttggtga taattgctat tataataaag aattttgtta
     4801 aaaaacctaa tttaccaggt tttgcatggg ctacattagt tgcatttgtt ttatcgcttc
     4861 caatatctcc agtgggggat atcatagcaa caaatgtagg gaaaatcaat tttatgtcta
     4921 cagtaacacc attattagct tttgcaggaa tctctgtagg taatcaatta gatatattaa
     4981 agactttgag ttggaagtta gtagttatat catttatagt tatgacatct acttactttg
     5041 gttctgcttt tatatctcaa acagttttaa gattaaatgg aatgctttaa ggaggtagta
     5101 aatatgaatg aattaaaagc aaaaattaaa gaggaaataa agttattatc tgaagagaaa
     5161 aagacatcat ttgaaaaagt ttctgactat ttattttcaa atccagaatt agcatttgag
     5221 gagtataagt cacaaaaagc attatgtgat ttgttagaag aaaatgaatt taatgtaact
     5281 aaaggtgtag gaggattaga aacatctttt gaagctgtgt attcaaatgg aactaatggg
     5341 aaaacagttg cttttttagc tgaatatgat gcacttccag gaatgggaca tgcatgtggg
     5401 cacaatatca tagggacaag ttctgttggt gcaggtatca tattaaaaga aatcatgaaa
     5461 aaacataata tagaagggac tgtaaaggtt tttggaacac ctgcagaaga aagagtaggt
     5521 ggaaaaataa ctatgattaa agaaggtgta tttaataatg tagatgctgc attaatttta
     5581 catccaagtg atgcttcaat gcctgatgat atatcatttg ctcaagttaa tttaaaattt
     5641 gattttactg gtaaggcatc acatgctgct gcttttcctt gggaagggaa aagtgcctta
     5701 agtggagtaa tagcactatt taacagtgta aatagcatga gattacatct taaagattat
     5761 gcaagagtac atggaattat aactgatgga ggtagcatac ataatataat tcctgaaaaa
     5821 tctacagcta tatttaatgt aagagcctta agtatagaat atttaaatga gatttgtgaa
     5881 atgcttaaaa attgtgctaa aggagctgca atttctactg gaactaatgt agaaatagtt
     5941 caattagatg aaatatacaa agagattaag aatgattctg aattagtgaa tatagttaga
     6001 caaaattttg aagttttggg tgaagactat gttgaaagag atttaagtca aggtataggt
     6061 tcaactgata caggaaattt aactcatgag atacctgcaa tacaagcata tataaaatta
     6121 aaagaaaata ctgcaactca tacagatgaa tttgctgttg cagctggtgg tgaagaaggc
     6181 agagtagcac ttataaaagc aattaaggtg ttagcaatgt gtggagtaga tattttgtat
     6241 agtaaataat atataatttt aaaatatatt aagaaaggaa atcggattag acatgatatt
     6301 aaaacaagta atagaggttt atgacatttt agataaagca aatgctaatg gggaagaagt
     6361 aaaaacttac ttacaaggtt atggtgaagt agatgtaact gtaaaagaat tgtcttcaag
     6421 taaaggttca acagatttag taaagcttac aattcctgga aaaaatggaa aattaaaagg
     6481 tggagatgct cctactcttg gtattttagg tagactagga gggattggag caagaccaga
     6541 agttataggt tttgtctcag atggtgatgg agcattggta gcaattgcag tggctgctaa
     6601 acttttggat atgcaacgaa aaggggatat attagatggt gatgttgtaa tttcaactca
     6661 tatttgccca gatgctccaa ctaaagaaca ctatcctact ccttttatgg attctccaat
     6721 tgatatgatg actatgaatg aaaatgaagt tgatagtagt tgtgatgcaa tactttctat
     6781 agatactact aaaggaaata gagtaatcaa tactagagga tttgcaatat cgccaactgt
     6841 taaagaaggc tacatattaa aaactagtga taacctttta gatattatgc agactgtaac
     6901 agggaaatct ccatttgtat tccctttgtc tattcaagat ataacaccat atggaaataa
     6961 tctgtatcac ttaaatagta ttttacaacc agcagtagct acagatgcac caatagtagg
     7021 agtagcaatt acaacagaga tgcctgttgc tggatgtgct acaggtgcaa ctaattttag
     7081 tgatttagat tcagctggga gatttgcaat tgaagttgcc aagctatttg gtagaaataa
     7141 gtgtgatttc tatgataaag aagagtggga aatgcttatt aagagatatg gcaaattaaa
     7201 tagatttcaa acatttggta tacagtaatt agaaaaatat taagtttata taatttgtga
     7261 tataactatt taagaatata taaacaatta ggaataaaag taagaataaa gcacttagtt
     7321 aaaaacttta aaaataaaat atttatttgt ttatataaaa aggaggattc tggttattgg
     7381 gattctcttt tttatgaata caaataatat attgtaatta taaaaaaagg agcgtttcaa
     7441 agtgattttt ttaatcacct tgagacaact ccttatattt ttatttaaat tctatttttt
     7501 attaagttgc tggagttcca ccaccatcag gaggagtagt agagccacca ccattatttc
     7561 caccaccatc aggaggagta gtagagccac caccattgtt tccgccatta tcaggaggag
     7621 tagtagagcc accaccatta tttccgccat tatcaggagg agtagtagag ccaccaccat
     7681 tgtttccgcc attatcagga ggtgtagaac cgccaccatt atcacctgtg ttattattgt
     7741 tattattatt ttcttctggt tggttattgt ctggtttttt tgtatcttca gatgatggtt
     7801 gagaacttac tttagtagga gcagtacctt caataaatgc tgctttagag ccagaagatt
     7861 gctttcctcc atcctctaaa ttaatttttg taaaatatac atttttagga acttcaaatt
     7921 cagtaaccgt taaattagca tgtatttttt ccataatttt ttcccatagt tttgcagtac
     7981 tagtacttcc atgtggtact tctctacgtt ttatagtgtt accagaatca tcttttctac
     8041 cagcatcatc tccaatataa gttgcaccaa cataataagg agtgtaaccg acaaaccaag
     8101 catcatatgc actatttgta gtacctgttt tacctgctac aggcattccc tttggaagtg
     8161 tagcaacacc accagtacct tcagtaatta cgctttctaa catatctgta acaacataag
     8221 ctacttcagg gtctacaact ttatgttctt caggagcatt tttaactaat aattgaccat
     8281 caaaggtagt tatagtagta aaaatagaag gttctacata aataccgtga tttgccaatg
     8341 taccataagc agcagccatt tgaagtggag taataccatt cgccatacca cctagtgtca
     8401 atgatgggaa ttttctatca cttgcttcag atgaacctga gtgattattc tttaaagttg
     8461 ttataccaaa gttctttaag taatccatca ttatatctac acattcatca taagagtctc
     8521 caagcatttc agcagttttg acagctatcg tgtttgaaga tttagcaagt gctttacgta
     8581 aactcataga accatgacca gcagtagtag ttcttggatt ccatttatta ttttcttcaa
     8641 acttatatcc tcctcgagaa tcacttagag cagttgcttg tgttattttt agagtatcaa
     8701 tagctggcgt atatattgct aaaggtttta tagaagaacc tggctgatga gggttagtag
     8761 cacgatttaa agtttttcta ccacttatat ttcttcctcc agctagtgct cttatttcac
     8821 catttttata atctaagatt accattgcag cttgcggttg gctaactcca tcaggaccga
     8881 ccatatgtcc tgggaaattg ctatttctat caaattcttc ttctaaagca tcttgcattt
     8941 tagggtctat agttgtgttt acaattagac caccattgta gaataagttt tgagcttcat
     9001 catttgtatg tccttgttcc attaaagcat ttataacttc actttcgata aggtcttcta
     9061 cagatgaaga aactttatct gatgattgag gcaattttat ttctatttta gctttgattg
     9121 cttcatcata ttcactttgt tttatatatt tcaattctaa cattttcttt aaaacaattt
     9181 cttgtctttt tttagcttca ggattactta cagcctttct aacaactaat gtacctgctt
     9241 ttaattgtct ataagtatca ttagatataa gctcccaaga ttttattttt tcaatcatat
     9301 caaagtccac ttgagtaggg tcatctaaat catcatcagt tgtgttaaca aaaaataata
     9361 atttattctc taaatcctcc ttagtttcat ttccctctaa cttagaagtt ttataggctg
     9421 aaaatcttga tggattttta gttgaaccag caagaagtgc tgactctgct agagtaaggt
     9481 cagctgcaga cttatcaaaa taaccacgtg caccagcctc agcacctgct agacctctac
     9541 caacgaagaa gttatttaaa taagtttcta gtattttatc tttacttact gttttactca
     9601 tctcatgtgc atagtaaata tcctttattt tacgaggtat agtttgctgt tcagttgtta
     9661 acagcatttt ggatacctgc ataggaatag tactaccacc ttgcttagta cctgtaaggg
     9721 tctttaatac tgacctaaat aaacctttaa tatcgacacc cttatgctca taaaaacgct
     9781 catcttctat tgcaattaca gcatgttgaa gataagggga aatcttatct aatggaacgg
     9841 gtgtcttttt atttacactt ggagctgtag ataatgtttt tccattagcg tatttaatag
     9901 tagttgtttg gttcgtctgt ttatctaaaa cagcttctgt aactggagtt acatctctta
     9961 gtgacgcaaa tactagacca gcagtacctg ctgtaccaac aactagtaaa actaaaaata
    10021 ctatcccaaa tactcttagt tttttaaatt tatcgctttt tttagatttt ttcttgtttt
    10081 ttgttgaacg agtcttgtta gctgaagttc tattaattgg tttatttgta ttaccagatg
    10141 agctaacttt ttttctcctg attttatttc catttttatc attatcgtta ttcataagat
    10201 tttaacctcc ttacttaatg caatattata acacaaatag gagtagtttt gtaattaata
    10261 gaaaaaatga atttgctata atattctgta cattgtatat gtataaacgt aatatattaa
    10321 aaagtctata tttttatttt atttattttt gacaaatatt ataaaaatta tgccaaagct
    10381 acatataaat aaataggagg ggataatttt gaatagaata gtagatagaa aaacaaaaaa
    10441 tgaatttaga aaaatcatag ctattttttt agtaattctt ttcttgtctg tatttatagg
    10501 cagttttata tatatagata aaactttaag accgacaata acagttttag cagagactaa
    10561 agctcttgaa ctagcaaata ggtcgataaa taaagctgtt gctgagatgg tagaaggaaa
    10621 aataaattat gaagatttaa tggatataca acttgataat aatggtaaaa taactatgat
    10681 acaagcaaac actataatga tgaatgaaat tgcatctgca atagctttag aaatacaaga
    10741 tgaattaaaa aaggataaga cagcaagttc ttacatacct ataggaacag cattaggaag
    10801 tcctatatta gctaaatacg gtcctaaact tgaagtatct attgaaccta taggaacagt
    10861 atcagtaaac tttaaaacgg aatttgagtc ttcaggtata aatcaaacta gacatagaat
    10921 atatttagaa gctcaaacac aagttaaagt agtaatacca cttataactt caacaaaaca
    10981 aattaaagct caaataccta tttgtgaaac tataatagta ggtgatgttc cagaaagtta
    11041 tgtaaatatt ccagaaaaaa atctaggaaa tgttttacct aacactggaa agaataccaa
    11101 caagtaatgt aaagttgtat atttttaaat aaattcaaaa taatagttta taaataaaat
    11161 ttttggaaat aatattaata taattttaat aaaaatttat attttatact atgtaaaata
    11221 tctaatatat ttggtctgta ggttaaataa aagtaccttt agaccaaata tattattaat
    11281 aacttaataa catcttaaaa cttatttatt tatattattt acaacatcca tttaataaat
    11341 acaccataac ctacagctgg gtcgttttca attgcatgtg acactgctcc gattatttta
    11401 ttatcttgaa ctattggagt accactcata ccttgtacaa taccaccagt ttgagctaaa
    11461 agctttttat ctgtaatttt aattttaaaa cttttacttt ctggatgttt ttggttttct
    11521 atatctataa tttcaatatt atatttttta cacttattaa agttatcttg taaaattatt
    11581 tgagcttttc ctaatttaac ttcatctaaa gatgctactt tatatttttt taacttggaa
    11641 atatcaaagt tattaatttg acctcttatt ccaaaatcag tattttctgt aaagctacca
    11701 ataatatttt ctcgttttgc atttatacat ccaacactac ctttataaga tttttctata
    11761 ttaaggtgct tagttgtaga aattgaacct gttttaatag gtatttttct tgcattacca
    11821 acacttattg gatgacctac agctccaaac tctgatgtat ctggattgat atacgttaaa
    11881 gtagcaaatc cagaaagtaa attgttaaaa ctaatttttt ctaatatgtg tttggtagtt
    11941 ttcattgtta taagttgtcc acctctattt aataaaatag atacttgttt ttcttctggt
    12001 aaagcattta aggtattggc aaagtcacta tatccatcaa cttcagcatt atttaccttt
    12061 actatagcat cacccaatct aaatggagat gcttctccag gatttctaac tatgatattt
    12121 tttaattctg cgtcaatttg aagtacgttt ccaattggta ttaaatattc aggttcattt
    12181 ttttgtaaat tttgtgcata aattaaatta tttgagaaaa aatataataa aaataaaaat
    12241 gttaaaacaa ttgtaaattt tgacttagct aagttggttt ttttacttaa agtattattt
    12301 ttaaaatgaa aatttttaag ttgcataaaa cacctctttc ttattaaatt attttaataa
    12361 tagaattgaa gttttcttgc tgcttctata ttatgttgtg caagaatcat gttttgatgc
    12421 ttggaaaact agctcaataa caccttagtt ttcataaaaa taaataaaat aaatagtgta
    12481 tatgataaaa taaatacata ttcatataaa taaaaataaa caataactgt ctagcataag
    12541 aaaggagcaa aaaatgaaaa aatatagatt gttagttact tgtataattg ccatgggaat
    12601 cataattata ggaacatcaa atccagttag caatttttac aagactacaa atttgattag
    12661 ttcaggaaat atcacaaatg aaaaagcaga taaagatgaa aattcaaaca aaaataataa
    12721 agataaaaat gaagacaaat ctaattctaa aagtaataaa gataagtcat cagaggacaa
    12781 atcgtcagat aataaaaatg aaattcaaaa aaataaaaaa cttttaattt gtatagaccc
    12841 agggcatcaa ggaaaaggag atagcaattt agaaccagta gctccaggtt cttcctctaa
    12901 aaaagcgaga gtatcttcag gaacagaggg tattgctaca aaaaagccag aatatgtttt
    12961 aaacttagaa gcatctcttg ttttaaagag tatattagaa tctaaaggtt ataatgtaat
    13021 aatgactaga gagacacatg atgtaaatat aagtaattca gaaagagcaa tacttgctaa
    13081 tgacaaaaaa gcagatatgg ttgtaagaat acatgcagat agtttaaata attcttcaaa
    13141 aactggagca tctatattga ttcctgaaaa agatggaaaa tatacagctc ctatatatga
    13201 agaaagtaat aaatgtgctg aatttatcaa acagaatatg gaacaatcag gtatacaaat
    13261 caatggaatc gttcaaagag gtgatttgac tggattcaac tggtcaaaag taccagctgt
    13321 attagtagaa atgggattta tgagtaacta taatgaagac caaatgatgt caaatccaga
    13381 ctaccaaaga aaaatgatgc agtgtatagc tgatggattg gatgcgtatt ttaaataaga
    13441 tatgaataga gaaaaagtta ggttggtatt atttgcctaa tttttatctc tattttttat
    13501 tgtacataac acaaagaaga agtttatttt aggttaaatt gtcgtaaata taactaaaaa
    13561 tattgggaat acttgattta agtttaaaaa gaatatatta tagtatagaa tgtgtaaaat
    13621 ttttttgaga ggtgttgaaa tgctagtagt agaaaatgtg agtcatggat ttggtgcgag
    13681 aacaatatta gaaaatgtat cctttagact tagaaaaggt gaacatattg cactagttgg
    13741 tgctaatgga gaaggaaaat cgagtttttt aaatataata acaaagaagc ttatgccaga
    13801 tgctggaaat ataaaatggt cttcaagagc tacagtaggt tacttagacc aacatacggt
    13861 attaagcaag ggaaaaacta taagagaagt gcttagagag gcttttaagc atatgtttga
    13921 cttagagcaa gaaatgattg ctatgtatga caaaatggga gaagctagtg atgatgaaat
    13981 gagtaagctt cttgaagaaa cagcagagat acaaactata cttgaaaata gtggttttta
    14041 tatgatagat gcaaagatac aagaagttgc aaatggtctt ggtcttggag aaattggtct
    14101 tgataaagat gtaactgact taagtggagg gcaaagaaca aaagtacttc ttactaaact
    14161 tctacttgaa aatccaacta ttcttatatt ggatgagcct acaaactatt tagacgaaga
    14221 gcatataaca tggcttacta agtaccttca agagtatgag aatagttttg tcttagtttc
    14281 tcatgatatt gaatttataa ataatacatg taatgtaata tatcatatgg aaaatggaga
    14341 gttaaataga tataaaggaa attatgatga atttgtaaga cttaatgata taaagaaacg
    14401 tcaagaagaa caagcttatg ataaacaagt tgaagaaaga aaaagactag aggattttgt
    14461 agctagaaat aaagctagag tagcaactag aggaatggct aacagtaggc aaaagcaact
    14521 tgataagatg gaaatattag aaagaccaaa agaaaaaata aaacctactt ttgcttttaa
    14581 agatgctaga gcagctagta aaataatttt tgaaacagaa aatttagttt tagggtatga
    14641 tgaagcattg acaaaaccac ttaatttcca tttagaaaga ggaaaaaaga ttgccttaaa
    14701 aggaatgaat ggtattggaa aatctaccct attaaaaact ttacttggaa taataaaacc
    14761 atttgaagga aatgttaaat taggtgacta tttggaagtt ggatattttg aacaagagag
    14821 ttcaagagaa aatagtaata cacctatgga tgaagtttgg agtgaatttc cagggcttac
    14881 aaactttgaa gttagacagg ctcttgcaaa atgtggactt acaaatgaac atataacgag
    14941 tcaaatgaga gtattaagtg gtggagaagc tgctaaggtt agactttgca aagtaatgtt
    15001 aaaaaatata aatttcttag tattagatga gcctacaaat catttagatg ttgaggcaaa
    15061 agatgaattg aagaaggcta tcaaagagtt taagggaaca gttcttttag tatgtcatga
    15121 gccagaattt tattcagaaa ttgttgatga tgtatggaat atagaagatt ttacaactaa
    15181 aatcgtgtag tgatttaact tattgctggg gggatttcat gaaaaagttt ctaaatgtac
    15241 ttttttcaat ttgtattagt attgttataa tagtaggtgt tattaatttt actgttggat
    15301 ttaaacaact ttattatttt gacattgatt acttaaatat atcagagtta tctggtttat
    15361 ctaaagatga tataaaattg aactacgatt atctaattga ttataactta aataagaatg
    15421 tgtctgagtt caaattacca acattaaaat cttctccaca agggaagata cattttgaag
    15481 aagtcaggaa tatatttcaa aatataaata aattagctaa attattattg gtagtttctt
    15541 tagtaggaat tatattgagt gtaaaaaata aaaatataaa aatcttaaaa actacatcaa
    15601 taacacttat aattatgcca ttattattaa cagttccaat tttattaaat tttgaaaagt
    15661 catttattat ttttcataaa ttattattta gaaatgatta ttggatattt aatcccgatt
    15721 tagacccagt tataaatata ttgccagagg aatttttttt tcactctgga atgatgattt
    15781 taatattaat tttgttagta agtatagtac tgtttgtaat gtataaatta tgtaaatctt
    15841 tatcaataaa aatatttcaa tgttaaatag agggatattt ttatttccaa aataagataa
    15901 taaaaaagga ttagattaaa agttatacaa taaaaatagc tgtgcaataa tatttagact
    15961 atgaaagtag ttgaaaaata tttatgcaca gctatttata atagaattta ctttttacta
    16021 gtgattttaa ctaaatcttg aaatttactc ataatgtaat tgtaattatc ttttttatga
    16081 ggtaatatac aactactgca atctttgata cctttatctg tatacctaaa gtttccacca
    16141 cagttttctc ctaaagcata caaaggacaa tagcaaaata gacagttaaa ctcttctggt
    16201 ttattagttt tatgacaagg gaaaaattcg caatctttgt gattaaaaaa cttataattt
    16261 tcgcccatat tttcctccta attaaataaa atttaaattt tttatactat ataagatttt
    16321 ggtgataata taattatact aaataatttt atgaatagtc gataatatag tattattaat
    16381 catagtatta atggtataat tagtcaacag aaaaataatg taaaaataat gtaagtgtat
    16441 tttgtaatta gaatatgttt tataacaaaa aatgaggtga ttaagtgaaa gaaattcaag
    16501 aaaaagcact tcttgtaggt ctgaatttga ctacaatagt gaaaaaaaat gatgatatag
    16561 acactaatga atctatggaa gaattaaaag aactaacaaa agctgctggg gcagaagttg
    16621 taggtagctt aattcaaaat aaacactctg tagatgcagc ttattatata ggaaaaggta
    16681 aagtagaaga aataagagcc tatagtgact ctttagatgc tactttagta atttttaatg
    16741 atgaattatc tggtgctcaa atcagaaata tagagaatgt tgttggaaga aaagtaatag
    16801 atagaactac tttaatttta gatatatttg ctcaaagggc acttagcaaa gaaggtaaat
    16861 tacaagttga attagcacaa ttaaaatata gattgccacg actttatgga atgggtggtg
    16921 agatgagtag aacaggtgca gggattggta ctagaggtcc tggagaacaa aaactagaaa
    16981 tagataagag acatatactt aataaagcag cagatataag acgtgaactt aaagaagtaa
    17041 aaaagaatag agagacacag agagttaagc gtttaaagtc aaatataccg attgtagcac
    17101 ttgttgggta tacaaatgca ggaaaatcta ccctgttgaa cgaattaata aagacacaca
    17161 aggattatga acaagaaaaa gaagtttttg taaaagatat gctttttgca actttagatg
    17221 taactttgag aaaggctctt ttgccgaata aaaaagaatt tttagtagta gatactgttg
    17281 gatttgtaag taaacttccc catgatttag tagaagcgtt taaggcaaca ttggaagaag
    17341 tacagtatgc aaatttaatc ttacatgtaa ttgatgcaac aaatactagt tatgaattgc
    17401 aaaaaagtac gactgaaggt gtattaaaag aacttggtgt aaatgataaa aaacatatac
    17461 ttgtatataa taaagtggac aagctagaat tggatatata ccctaaaagt caagaagata
    17521 ttgtgtatat ttctgcaaaa caaggcataa atatggataa gttattaaat atgatagaaa
    17581 ttgctttaat ggaaaatact tattcagtaa gtttgatgtt accttatgag agaggtgata
    17641 tatttagtag aataaaagat aaatataatg tggagaattt tgagtatggg gaaagtggta
    17701 taacgctaga tgcagattta gatgaagaag attttaatat ctatagagag tacatactag
    17761 aaaaataggt tgaatatgga tataaactgg gaaaatatag ataatcttga agattatttt
    17821 ataacctatc ttctttataa agaatctaaa acagtatctc aaattagtaa aattagaaat
    17881 atttcaagta ctgaagcaag tgagcaactt atacaagcaa agttaaaaat aaaggaaatg
    17941 caaaaagatg attttgaggc atctaaggat attttggata aatttctaga actagataaa
    18001 attaagagac ttgattttat ggatagctta gatgacgaaa aaatggtata ctttaagaga
    18061 aaggttttca aaagaatcct tgtagaaaaa aatgctgaag atttaattgt tttaatttgg
    18121 gcgacaggtg agctaaaaga tgataggttt ttgaaattgc ttcatcagct cacaaaccac
    18181 agacactcag atattagaag aataacttat tcagcaatca gaaagattga atcaccaagt
    18241 agtagagaag tactgcaaaa aggtctctac gacaaaaatg cacagactag gcagtactgt
    18301 gcaaaagctc tttcaaaatt gggagatgaa aacagtttaa agattttaca gcagttgaaa
    18361 gacaagaata agaattttga aaaagaatat gtgcttagag cttatgacga agccattaag
    18421 tcattggaaa atgcaaaaaa tttgaaaaag taatttttta ttaaagcaat ttgtttactt
    18481 aatgatgtat ttctaaagtt taacaaggta ataaataaat taatgggagg ggtttcaaat
    18541 gatagtaaga cgttgtgcag gtggagtagt gttttatgct aataaagtac ttattgtaaa
    18601 aaatgatagg ggtgaatgga cacttccaaa aggaaagata ctaggagggg gacttccata
    18661 tgagagtgct gttcaaagag ttaaagtaga aactggcata gatgctaaaa tgatagatgt
    18721 ggctggcgat actatgtatg aatttttctc aagaagtaga caacaagaag tttgtaatgc
    18781 aataatgtgg tatgttatgg aagcttgtaa cactgattgt gtacttgctc ctgaatttca
    18841 agaaggtgga ttttataaag taaaggatgc tttagaaatg ttatcacatc ataaagagca
    18901 agctttagta gaagtatctt ataaaaagtt taaggaatta aagaaattag tttcagaggg
    18961 cgatgatgtt ccagcaaatt aattgtgaat ttagaggatt ctgaggaagg gatagcccct
    19021 tcctttttta attgaagcag ttttaagcgt aaattatata gtctctttaa actatgattt
    19081 atcaatttag agatttagta gtttatattt aaaatcagta agatatattt aaaataaaaa
    19141 ttaaaaagta tacaatttta ttatataatt gtcttatgat gttgattaaa gaagtattaa
    19201 aagtccattt atttttaaat agaatgattt tataaatata tatttgatgt gtatattaga
    19261 aaatgattaa aataggagga aatacaatga gcaattacag gacattacat gaatttggaa
    19321 cggatgaaat aacgattgaa aagtctgttt ttattggata tgctaagcct ataaagagtg
    19381 aagaagaagc attagagttt ataaatgaaa taaagaaaaa gcataaagat gcaactcata
    19441 atgtgtgggc ttatactgta ggtaaaaata tgaatataca aagatatagt gatgatggag
    19501 aaccacaagg aacagctggg atacctacat tagaggttat aaaaaaagaa gatttgagag
    19561 atgtagcggt tgttgttact aggtattttg gaggaacaaa attaggtgca ggtggtctag
    19621 ttagagctta tacaaaaggt gctaaactgg gacttgaagc aggtaagata atttataagg
    19681 taatgtatca agaagttaag gttaagatag attatactca acttggaaaa gtgcaaaatg
    19741 agttaatgaa tttggggtat tttataaaag atactgtata tgaggataat gttgaaatag
    19801 tagtatattc aagattagaa gatgtagaga aattatcaga aaaaatgatt gatataacaa
    19861 gtggaacagg aaaaatagtt ttaggagagg aattttattt gtctgaacaa aatggagaaa
    19921 ttttaatata atctaaaagg ttattagaat agttaatacc tcaaatagcc aattagtatt
    19981 tttaagtgaa aagccattaa attgatttaa tctaaatcaa tttaatggct ttttatcgga
    20041 ttttgcacag aatccttttt ttacttcaaa taaaccgact aaaagtaaaa atatcccaaa
    20101 tatttttttt aatatatctg aagacagata attagctaat agtgaaccaa atacagctcc
    20161 taaaacccca aacacagcta taggtataac caatttaaaa attacatttt tattttttat
    20221 atgtatagca agtgctataa gtgtcatagg gatagatgaa agtaaattta cgctttgtgc
    20281 tattttagtg tctatgccag caagtaaaac gagtgcaggt ataaggattg ttcctccacc
    20341 cattcccatt cctccaataa ttcctgcaaa aaatccaatt agagcaaata acattaaaac
    20401 accatcctta atgctgcaat tatcataatt attccaaaag aaattctaag aaactttcca
    20461 gtaaccttat ttagtaattt agctcctata ataccaccaa gaatacttcc aactgcaact
    20521 ttaattgtta agttaacatc ataagtacct ttagatacat ataaaacaga acttgctgtc
    20581 gttaagaata ttattataga aagagcagtt gcatgagatt tatgttcttc taccttaacg
    20641 atgtcattta atattggaac taaaagagta cctcctccag aaccaaaaac tccatttata
    20701 aatcctgtaa aaactccaat tatcgtattt tttaaattaa aatcaaagta ttttttcttt
    20761 aaatcatcag tttttttcat aaaaatcacc ttctgaaata gttttaccaa aataggcaaa
    20821 ttatatttat ataaaataag attaaatata atctttcatg tttgtttaat tttgcataaa
    20881 aaaaaacata ttaaaacaac atattaatat atggtataat atattaaaac aaagtagcaa
    20941 atttgagagg agataacaat gtcaaatata aaaatacaaa tagacaaaac tgtagaatgg
    21001 cttataaata aggttaatga ggcaaatgca aaaggattaa ttgttggtgt atctggagga
    21061 attgattcgg cagtagttgc taatttaatt aaaaaggcat ttccagaaaa ctctatggga
    21121 gttataatga gcataaaaag taatcctcaa gatagagaag atgcacttaa ggttatagag
    21181 ggatgtgata tagaatacct tgatttagac cttatagaac cacaaagtgc tatattagac
    21241 atggtagtag gaaatttaaa agataaacat ttatatagag aagaatattt aaagatgaca
    21301 gatgcaaact taagagcaag agtaagaatg agtacaattt atacaattgc taacaatcta
    21361 ggatatctgg ttgttggtac agacaatgct gcagaaatac acacaggata ttttactaaa
    21421 tttggtgatg gtggagttga tattttacct attgcaaatt taactaaagg agaggtgtat
    21481 gaatgggcaa aagagcttgg tgttcatgaa gatttaataa acaaagctcc ttcagcaggt
    21541 ctttgggaag gtcaaacaga tgaagatgaa atgggaacta cttacaatat gatagatgct
    21601 gtattagaag gtagattgga tgaagttcct aaaagagacc aagagataat tgaaagactt
    21661 catagattaa gtgaacacaa gagaaaaacg cctgcacaac ctcctaagtt ctaatttact
    21721 atttataaga tttgtgataa aatttacaag tgatgaaaat tagaataatt aattgtacgt
    21781 attaaggagt ggaaatatgg gacgtatagg taatataatt aatagaaaag gtaaacaaga
    21841 tgctcaaaga gcaaagatat ttactaaaca tgctagagca atagcagttg cagctaagga
    21901 aggtggagca gacccagagt acaatgcagc gttaaaaaca gctatagaaa aagctaaggc
    21961 agataatatg ccaaatgata atatagatag agctatcgct aaaggtgctg gagcaggtgc
    22021 tggagaagac tatgaaacta tagtttatga aggatatgga cctggtggag ttgctgttat
    22081 agtagaaact ttaactgaca acaaaaacag aactgctggt aacgtaagat actattttga
    22141 caaaaacgga ggaaacttag gaactagtgg atgtgtgtct ttcatgtttg ataaaaaagg
    22201 acaaatatta gttggtttag gagatggagt ttcagaagaa gaattgatgg atgttgcatt
    22261 agaagcagga gctgaagatt tcataacaga agaagatggt tatgaaataa taactacacc
    22321 agaagatttt tctagtgttc gtgatgagtt aaaagctaaa ggatatgagt ttatatctgc
    22381 tgatgtgaag atgatacctc aaactacaac tgtattgact gaagaatctc atttaaaaat
    22441 gatgaataaa ttagttgata tgcttgaaga agatgatgat gttcaagata tatatcataa
    22501 ctgggaagta gaataaatag tataagcata ttgaaattta taagtattga atcaatttta
    22561 acatgtgata aattttatag aataagaaac actaaaatag acatacgcac ctctaaccta
    22621 tatttgcata ataatatgta agcataggta aggggtgttt ttatatttat aggttaagaa
    22681 atttaaagta aattttgagc tggactcgat tgtctataaa taattgatgg gtatataact
    22741 tgttgctgaa taccatttat acgcccagtt actgtatcaa taatcatcct aagactacgt
    22801 tctgctgtct cttccatttt taaatctata actgtcaaag gaggaatact tcttgccatt
    22861 agtcgtgaag gtccattact tgctgcaaat atttgaatat cctctccaat tacaattccc
    22921 gattcatgaa atgcattcat cataccaaga gctaccacat catgattaca aaaaattact
    22981 ttttttagtg aattttttgc aagcattttt tgagcaatat catatccact atctatttta
    23041 ttagaacaat aatatataga atctgataga tgtattccat gttctttaca tgtttttaaa
    23101 aaagatttac cccttaaagt catacatgta aaggaagttg ggttgaatac aacagcaata
    23161 tcatttttac cattttgaat agcatgtaaa gctgctatac gaccagtttc ctcattatca
    23221 atagatacac ttgaaaaacc aggtatttca cgatttacaa caactacagg aattttaggt
    23281 ttatttttta ctagatattc tatatcatca gcattgatac ctgttaaaac aattccatca
    23341 taataattat ttgcaaaaaa gtattcatca tcacatagat gaccaacttt gtaaggtcgt
    23401 atgatgatat ttatgtttaa atgttctaaa gaaattatgt ggtttattgc ttgaagtata
    23461 ttggttgatg ccaactcaaa tccctcatcc tgccaataaa aagctatttt aatattatta
    23521 tgtttaatag gagtagagcg tttatatcca agcttatcag ctgaatttaa tataagctct
    23581 tgtgtatgta gcggaatcct gcgttcattt cctttaccac ttaagacgta cgagacagtt
    23641 gctgaagaaa gaccacagtc ttttgcaata tctttgattg ttgccatgat aaaatcctcc
    23701 ttaaatagtt gcttaaattt gcttatacca ataatcttaa catatattca actaaagaac
    23761 aatattctac aaggtgcata ttttacagga ttagaataaa taaattaaaa aaaacttctt
    23821 acttatgttg acataattta gtttatagga ttataatatg cttaaataag cttatgatta
    23881 acttgaatta agcaaagtta agcaggaaca tgcaatgcta gcttgcaatg tgactgcaaa
    23941 gggggaaaag gtatgagtaa ggtaaatata tttgaagttg gtcctagaga tggatttcaa
    24001 aacctgaaag agtttttaga aactgataaa aagcttaaga ttattgatgg tcttattgct
    24061 tctgggatta aacaacttga aattagttca tttgtaagcc caaaagcaat acctcagatg
    24121 aaagatgcaa aagaagtagc aacttattgt gtagaaaaat atcctgatgt gaaactttat
    24181 gcattagttc caaatttata tggtgctaaa gttgcatggg agtgtggtat aagagatata
    24241 tcctatgtta tatctttgag tgaaactcat aataaaaaaa atataaatcg tacccatgaa
    24301 gagtctattg atgagcttaa aaaaataatg aatacttatc cagacatgaa tatatgtatt
    24361 ggaatggcta ctgtatttgg atgtcctttt gaaggtttac caaaaattga agatgccata
    24421 agatttgcta aacgcctttg ggaaaacggt atcagggaga taaatctagc agatacaata
    24481 gggattgctg accctaagca agtaagagaa actattagtg cattagttga agctttacca
    24541 gaatgcagat ggcaagtaca tattcatgat actagaaata tgggaatggt taatactctt
    24601 gtggctattg aatctggaat tactactgtt cagtctacac ttggaggatt aggaggatgc
    24661 ccatttgctc ctggagcatc tggcaatact gcaactgaag atttagtata tatgcttgaa
    24721 cgtatggggt atgatacagg agttgatttt gttaaacttc ttgaattatc aaaatatcag
    24781 aagagttttg taaatggagt atatagtggt catcatgtaa atataaagtc tgatagttgt
    24841 aattgttagt tctattgatt aatcgattaa aaaaatatgc tgattggtaa aatagattta
    24901 ttgataaaaa atataaatta taaaatacat tatctaataa aacaaataaa aaaattgagg
    24961 gggattagta tggatattat agctttactt ttatttttag gagttattat tattgcattt
    25021 gtacgtaaga ataatgcagg tattttggct ctagctattg gtgtcatagc tgttagaatt
    25081 tttggaatgg atgataaaga actttatgga gctataagca actcaatgtt tactacactt
    25141 gttggtataa cactactctt tgcaatagtg aactcaacag gagcattaaa gcttgttgca
    25201 gaaaaaattg tagctgcttc tggtaaacgt gtttggctta tacctatagc agtttttata
    25261 gcaggatttg ttgttgctgg tgttggacct ggggcaattc cagcacttgc aattatccca
    25321 gcaatggctg ttcctgtagc cataacagtt ggttataatc ctgtaatgct tgcacttata
    25381 ggtgaatgtg gattaatggc aggtcgtatg actcctatta ctccagaagg tcaaattata
    25441 acaaccgttg cagaaggggt tggaattgaa aatgttatgc ctactatctt agcctgtcaa
    25501 actctttcta caattgtatt ttcaatagta ttgtttataa ttttcaaagg atataaactt
    25561 aaaaaaccta taaatgttat agatagtaag caaatagaaa aattcaatat aaagcagata
    25621 atttctctaa taagcatagt agttatgatt gcttttatca taatttttga tatgaatgtt
    25681 gggcttgcag cttttgcagt ttcagctgta cttatttttt tcaatattgc tgatgaagga
    25741 gaatgtatca aatcaattcc atggagcacg atagtaatgg ttcttggagt aggtgcaatg
    25801 atgacaatcg tagatgcagc aggaggtatt gacttgatga gtaatgcatt atcatcactg
    25861 atgaatagta aaacagctac tccaattatg agtatttcag caggacttat gtctatggtt
    25921 agttctgctt tagcagtagt ttatccaact atgatgccta tgtgtgttga catagctaag
    25981 gcagttggag gagtagaccc actagcactt attgctgcag ttggtgttgg aggttctctt
    26041 gctggtgtaa gcccactttc tactggtggg gcattgattt tagcagctat gggttcaagt
    26101 aagaaagact ttaataagga agaacaaaat aaggtttttg tacagctttt cattatgagt
    26161 gcagttggct tattggttat tgcagtagta tcaatattat catttaatgc aataacacat
    26221 ttaatgaact aattagaata actgaaaata aatcattaaa tttaactttt aatattaatc
    26281 aatataatat aatttggagg ggaagttatg aaaaaaggag cattaagcaa tataaaggta
    26341 cttgacctta caagagttct tgcaggtcca tattgtacaa tgatgttggc ggattttgga
    26401 gctgatgtta taaaaataga gatgccaaga aaaggtgatg atacaagagg ttttggacct
    26461 tttgtaaatg gagagagttt atactatgca aatataaata gaaataaaaa aggtatcact
    26521 ctaaacctta aatcagaaga aggaaaaaat atatttttag aaatggtaaa acatgctgat
    26581 gttgtagtag aaaattacag accaggtgta atggaaaagt taggtctggg ttatgatgag
    26641 ttggctaaag ttaacaatca aataatttat ggagctgtat caggatttgg atgttatgga
    26701 ccatattcag aacgtccagg ttatgatatt attgcacagg caatgggtgg tcttatgagt
    26761 ataacagggc aagaaggagg agaaccaacg agagttggta atgctatggg tgatgtacta
    26821 ggtggaatga atttaactat tggtatactc acagcactca acgctagaaa aataatagga
    26881 aaaggacaaa gagtagatgt ttccctcgtt gattctgttg tatcgtctct cgaaactggt
    26941 atacagagat actttgattc aggacaacct gaacttatgg gtaatagata tgcttcagcc
    27001 tatccttatg actcttttaa ggcaagtgat ggaagttttg taataggctg tggtaatgac
    27061 aaactttttg aaaagttatg taaaaaagtt ctttttagag aagacctttt aatagatgag
    27121 agatttaaaa caaatcttaa tcgttgtgaa cattacaagg ctcttaaacc agaaatagaa
    27181 aaatggtctg ttaagcatac aataaaagaa gcagttgact taatatcaga ggctggagtt
    27241 cctgctgctc ctatctataa tcttgattgt gttacaacag atgaacatat agcaaaagca
    27301 agagaaatga ttgtagatat tagtcatcct attataggaa atatgaaagt caatggaaat
    27361 ccagttaagt tgatgacaac aaaagctaaa attgatatgc cagcaccatc tctaggggaa
    27421 agtaacgagg agatttataa tggttggctt ggttttgata gtgataaaat aaaagattta
    27481 aaagaaaatg gagttatttg attaaaaaat agctaaacac acttgtgact ttagtcatgg
    27541 gaggttcagt tagaataata tcaaaaaata ttaaatcaat actgtaaata aaatatagaa
    27601 ttgtcagaag catttaagat gggaaaagct acttagacat tgagtctttg tggcttttcc
    27661 tattttcatt aagaatttta tattatcaga atagatatgt actaaactaa agtattaaca
    27721 atttaaatta atagaaaata aactacctaa ttatgttgaa taaagactac tttagtgaaa
    27781 ataaacttat agagatgcta tttaaattat tttaatggaa atatcaaaaa tgagactgca
    27841 ttctcaaaaa tgagactgcg ttttttaagt attttctcga aaatgagaca aaaatattgt
    27901 tttatgggtt ttattgaaga aaaattcttc tgtgaattac aatttaatca attttaaagt
    27961 tgaaaattat taaaaattta gtgaaattta tgtatatttc gtttatttta ttaaaaataa
    28021 caatttttta ctaaaaataa agaacttcaa tggtgattgg catagtactt gctattttat
    28081 attaacaaga aatgtagaaa aaataacaat gtataatggg gggaatatga tggaatctgt
    28141 aatggaaaat ttgaacaatc ttaaggtgga ggttaaggac aaagtctgtg ttattactat
    28201 aaatagacct aaagctttaa atgctcttaa tagtgacact cttagggaac tatcacaagt
    28261 tattgatgta gtatcagaaa atgaggcaat attaggagtt attattactg gagaaggtaa
    28321 agtttttgta gccggtgctg atatacgcca gatgcaaaac tataaaagtg aggaaggcag
    28381 aaaatatgca ggttatgctc agggaatttt tgataaaatt gaagctcttg agaaaacagt
    28441 aatagcagca gttaatggat atgcattagg tggtggatgt gaacttgcta tgagttgtga
    28501 tattagaatt gcatcagaaa aggctatatt tggacaacct gaagttaatt taggagttat
    28561 tccatgtttt ggtggaactc aaagattatc aagacttgtt ggaactggaa tagctaaaga
    28621 gttaattttt acggggagac aagtaaatgc agaagaagct aaatctattg gacttattaa
    28681 taaagtggtt ccaagtgatt tacttttaga agagtctatg aagatgatga accaaatagt
    28741 tgaaaaagcg ccaatagcaa taagatatgc aaaagttgtt attaacaaag ggatagatat
    28801 ggatttaaag aatgctcttg aactagagaa agatgttgca ggtttaactt ttgctacaag
    28861 agataagcaa gaaggaatga atgcatttat agaaaagaga aagccagttt ttgaaaataa
    28921 atagctatta taactaaaat tggaacaatg tagaatatat tcaaaatcta aatttcgtga
    28981 aaaaatacat atagtttaat tttaaaagga gttaatttat gaatacggga gtaattataa
    29041 gtttagtagg gattatcata gctctatctc ttctagttgt aatggttatg aagggaataa
    29101 atatttttat tatagcaatt gtatgttcat cattagttgc tataacagga agcctaaatg
    29161 tgtatgatgc acttaaagtg gattatatga cagggtttgt tggatttctt caggcaaatt
    29221 tctttatttt cctaacaggg acattaatgg gaaaaataat ggagattact ggtggagcaa
    29281 aatctattgc taagatgatt gtaagatgga ttgggaagga taaagcacta ctttctattc
    29341 caatagcttg tggaatatta gcctatggtg gagtaagtgt tttcgttgta agttttgcag
    29401 tgtttccgat agctttagaa atttttaaag aagctgattt gccacgaaga tttataccag
    29461 cagctttgac ttttggatgc tcaacttttg ctatggttgc accaggggca ccacaaattc
    29521 agaatattgt tccagcatca acattaggga cagatattat ggctggagca gtaaatggtt
    29581 ttatatcatg tgcagtaatg tttatagttg gaagtacaat tttgtacaaa atggtttcaa
    29641 aagaaaaagc caatggtggt cattttatag cacatgaatc agatatattt gatgaagcag
    29701 aagcaactaa ttctaagaga gaaaatggtc ccaatggtgt aattgcctta attccattga
    29761 tagtgtcgat tttaataatt aatgtaaaag ttaatggaaa agcgatagta ccaattgagg
    29821 tgggagtatt tattggagct ttattagtat atttattgtt aaataaatat caagataata
    29881 ctaaaatagt tggacatgtt ggtgatgcat gtaaaactac agtagtagca atatgtaata
    29941 cttgtgctgt agttgcattt ggttcagtag taaaatcagc agttggtttt gacttcatag
    30001 ttaatgctat gactaatatt cctggtcctc ctattgcagg ggctgcagtt ggaactacag
    30061 ttattgcagg tatttgtggg tcagcttcag gtggactagg aattgcagct ccacttcttg
    30121 gaccagtatt tttagccaag ggtgtttccg ctgcagcatt aactaggact atggcaattt
    30181 catcagcagc attagattca cttccacaca atggatatat tgttactgta acaaatggtc
    30241 tttgtaaaga aacacataag gatgcctata tgccaatatt ctgggtaact gttttaaccc
    30301 catttattgg aaccatagta gcagtcctac tgtttacaat gttcccggga ttgccatagg
    30361 agaaaagata aaaggtataa attatatttt gatattataa aatcaatttt caaaatatat
    30421 acaataaatt tcatgtttta atactgtaaa tatgtattta aaataacaaa ttgaccatat
    30481 aaggaggaat tgaaagtgtt aaataaacca ttagagggag ttaaggttat tgacctaacg
    30541 tattttgtag caggtccagg gacttctaaa atattagctg actggggtgc tgacgttatt
    30601 aaggtagagc caagctttgg agacccagga agaaagacag gggcaacaat gacaatgcca
    30661 atagatgatt ataataatcc tttttacagc acatataatt caaataaaag aggtttgtct
    30721 ataaatctta agagtgaaac tggaattgaa attatggata agcttttaag cgaagcaaac
    30781 gtatttgttt caagttatag aacaggagca ttaaaaagac taggtcttga ttatgaatca
    30841 ttaagtaaaa agcatccaca ccttatctgg ggtcagataa atggttttgg cgattttgga
    30901 cctgctaagg ataatgcagg atttgataca gttgcctttt gggcaagaag tggggctatg
    30961 cttgatatag cagaaaaaga tacaagtcca ataaatccac ctatagcctt tggagatgca
    31021 acaacttctt gtagtttatc tggaggaata tgtgcagctt tgtaccagca agctaagact
    31081 ggaaaaggac aaaaagtaat ggcatcttta tttgggcaag caatatggaa tgcatcatct
    31141 ttattagcat ctacacagtt tagtgatgag tatccaaaaa ctagaaaaaa tgctatttca
    31201 ccagtaataa attcttatcg ttgtaaggat ggaaagtgga tattcttaag tattttagag
    31261 catgaaagat attttgaggc actttgtaga ttatttggaa gagaagactt aataaaagaa
    31321 gagaaatttg catcttcagt agaaagtaag aaaaatgcta cagaacttat taatatttta
    31381 gatggtgaat ttgcaaagtt tactcaaaat gagatggttg aaaaacttac tgctgctgat
    31441 atagctcatg aaaaaattca acatgttaat gatgtagtga ctgatgaaca ggctattgct
    31501 aataactata tatatgaaca tacaagtaaa gatggaaata agacaatgct tgcatctact
    31561 ccagttaaat ttggaaatat tgaagttaac atgacttgtg atgctccact tataggagaa
    31621 cattcagatg agatattgag agaacttgga tacagtgatt ctgatataga gactctaatt
    31681 gaatctggaa ttgtaacaat gaataaagaa gcatgtgaaa tatgttaata aacatgtgaa
    31741 gtatgttaat aaataggagg gaattttatg gattttagat taacagaagc acaattaatg
    31801 cttcaaagag tagcaaaaga atttgcagag aatgaaatag caccaatagc tgctgaaaca
    31861 gataaaacag gtatttttcc aagggagctt tttagcaaaa tggctaaaat aggatttaat
    31921 ggaattggaa cacctgtaga gtatagtggt tctggtgggg cagatataga aaaggtaatt
    31981 gttgttactg agattgccaa aaaatgtgca gcatctgcag caatattatc aatacacaca
    32041 atatatgctc aagctatttt gaaatttggt actgaggaac aaaagaaaaa ataccttcca
    32101 atgatggctg aaggtgggtg tgtaggtgct tttgccttaa ccgaacctaa tgctggttca
    32161 gatgctgcga gagctgcaac aactgcaatt attgatgaag aaactgatga atatgtttta
    32221 aatggaacta agtgttttat ttctggtgga ggtcaagcag aatcattaat aatatttgca
    32281 ttgacagacc catcaaaggg aataaaggga atgtctgcta tcattgtcga caaaggtact
    32341 ccaggttttt caataggaaa aattgaagaa aaaatgggta ttcatggttc agaaactgca
    32401 gagttaatat ttgataactg tagagttcca aagtctaact tattaggaaa agaaggtaaa
    32461 ggatttaata tagctatgac ttgtttagat ggagcaagaa ttggagttgg agctcaagct
    32521 gtaggaattg ctgaaggggc tttagaagaa agtatcaaat attctaaaga aagagtccag
    32581 tttggcaagc caatttcagc tttgcaaggt attcaatggt atatagcaga tatggcgaca
    32641 atggtggaat cagcaaaatt attagtatat tatgctgcag atttgaaagc tagaggagaa
    32701 aaacacacta aagaagctgc tatggcaaaa tacaatgcgt cacgtactgc aagagaggtt
    32761 acaaatttag cccttcaaat ccatggtgga tatggatata tgaaggatta tccattagag
    32821 agaatgtaca gagatgccaa aataacagaa atttacgaag gtacttcaga aatacacaaa
    32881 gttgttattt caagagcagt actaggatag gaggaaaaga atgaatatag ttgtttgctt
    32941 aaagcaagtt ccagatacta atgaagttaa aataaataaa gagacaggta ctctaataag
    33001 agatggtgta ccaagtataa tcaatccaga tgatagaaat gctttagaag aagctctaaa
    33061 aatgaaagat gaactaggag cagtgattaa ggttataagc atgggacctc ctcaagctaa
    33121 aagtgcttta aaagaagctt tggcaatggg agctgatgaa gcttatttaa tctcagatag
    33181 agcttttggt ggttcagata cttgggctac atctacaatt attgctgctg ctatagagaa
    33241 ggttggaaag tatgatgtaa tattctgcgg aagacaagct atagatggag atacagctca
    33301 ggttggacca gaggttgcag aatttttagg gataccacaa gttacttatg cgaaagaagt
    33361 aaaggttcaa gatgacaaat tactagtaac tcgttataca gagacagggg attatttaat
    33421 agaagcaaag atgccagttt tattaactgc tataaaagag ttaaacaatc caagatatcc
    33481 aagtgtcaag ggaatcttag aagcttataa taatggagat gcaaagatta ctgtattaac
    33541 acttgcagat ttagatgttg atacaactca aattggatta aagggttcac ctacaaacgt
    33601 ttataagtct tttgtaccag ttaaagacaa gcataatgaa ataatagaag gtataaataa
    33661 aaaagaaaaa gctgagaaac taatagaaat actattcgat ttgaaactag tataggagtg
    33721 ttaagatgat gagagcaaaa gttaatcaag gtataaattt aaatgattat aatggtgttt
    33781 gggtaatagg agagcaaagg gaaggtaaaa ttaatcctgt tacgatagaa cttattggtg
    33841 aaggaagaaa gcttgctgac caattaggaa aagagcttgc agttgtgata gctggatatg
    33901 aagtagagaa agaagtgaag gaattacttc actatagtgt tgataaagta tattacatca
    33961 atgaccctct tcttaaagat ttcactactg atggatatgc aatttcaatt gcaaatttaa
    34021 tcgaaagaaa aaagccagag gttgtattgg ttggtgctac ttctatagga agagatatag
    34081 cgcctagaat tgcaggtaag gttggaacag gtctaactgc tgactgtaca aaacttgaaa
    34141 tagattcaac agataataag ttattacaaa caagaccagc atttggtgga aatttaatgg
    34201 ccactattgt ttgtccaaaa aatagaccac agatgtcaac agttcgtcca ggagttatgg
    34261 caaaagctgt aagaaacgaa tcagagactg gaattttaga agttgttact ccagaactta
    34321 ctgaaaaaat gattagaact agattagtag agatacttcc acaagaaaag aaatctgtaa
    34381 atctaactga tgctagaata atagtatcag ggggaagagg attaaaaaga gcagaaggat
    34441 ttgagcttat aaaagaattg gctgataaat tgggggcaga gattggtgct tcaagagcag
    34501 cagtagattc agggtggata gaacattctc atcaagtggg acaaacagga actactgtta
    34561 gaccagaact atacattgca tgtggaatat ctggagcaat acaacattta gcaggtatga
    34621 gtgattctaa atatatagtt gctataaata aagatgcaaa agcccctatt tttagtatat
    34681 gtgactatgg aatagtagga gatttatatg aaattatacc tgaaatgata gaatcattaa
    34741 ataggtaact tttagaaaac attgaatatg tagaatgata agtcttataa tattgtattg
    34801 taagacttat tatttttgct gaaggttata gtggggagat gaaacaatga aagagcagtg
    34861 gtataaggat atatttgcaa gagtattatc tatgactgat gatggtttta ttgttgtaaa
    34921 tacaagtgga gtaataatag atattaatga taaatattgt gactttttag gaaaagaaag
    34981 aaaagatata ataggacaaa atatacaaag tattatacca aatacaaaaa tgctagatgt
    35041 tatgaaaaat aagtattgtg aagaaggagc tatacatcat tattcaggtg gaaatacaaa
    35101 agaaaaaagt gtaatagtaa gtaggtctta tgtggaaaat gacaatggag aagtagttgc
    35161 aggagtagct caagttaaat ttagattgca atcatttgat gtagctaaaa agttaatgtc
    35221 agaatatatg gagctccaat attataaaga gcagtttaag gataactgtg gttttgacaa
    35281 gctcataggt gaaaatagag acttcataga acttaagaaa acaggtgtga aagcttctaa
    35341 aacaaacttt ccagtacttt taacgggtga aacaggcaca ggtaaagaag tttttgctag
    35401 agcaattcac aataatagca gtaggtcaga taaacctatg gttagtataa attgcgctgc
    35461 aataccagaa gaattattag agtcagagct atttggctat gacgaaggag cttttacagg
    35521 agcaaaaaag ggtggtaaaa aaggtaaatt tttagttgcg aataatggta caatcttttt
    35581 agacgagata ggtgacatgc ctttgaccat gcaagcaaaa cttttaagag ttcttcagga
    35641 aagtgagata gaaccagtgg gaggtcttaa aactataaag atagatgtaa gagtaatctc
    35701 tgccacaaga aagaatttaa gtaaaatggt tgaagaggga ttatttagag aagacctata
    35761 ctatagatta aatgtaataa acattcatat gatggaattg aaggacaggc aagatgatat
    35821 tttattgcta gcaaattata tattaaacaa attaaatgtt gaatacaagg aattgaaggt
    35881 tttaagtgat aaggttaaaa attgttttat taactatact tggccgggaa atataagaga
    35941 actacaaaat gtaataaaaa gtgcctatgc tgtaagtgat gatatggtca ttatgatgtg
    36001 tgaccttcca tctaagatgg ataatatcag cagagtagct caatgtaatg tggatagcaa
    36061 ttgttcaatt catgaaatgg ttgagaatta tgaaaagagc ttaattattg atgtgttaag
    36121 gaagtataat tggaaatgtt ctaaggcagc agaggttatg ggaatacaca agagtctttt
    36181 atataagaaa ataaaaaagt atgaaattga attaaataat taagataact tgaattaagg
    36241 caatgctaaa aaagcgccct tattcaaagc gaactgtata aactgtttta tgcagttaaa
    36301 acgtgtaaaa gtagatgtga agtactaatg tcaatcatta gtactttttt attatattta
    36361 taattactat ctatatttca gtatcttttg atatctaaga ttattatgtt taatttagta
    36421 tataacaaaa ccctcttagt tatggacagt tttttatatt ttaactgtct actaagaggg
    36481 gatagtttag tttaaattta gagtaaattt tatctagcat ttataaaaaa tattttgaat
    36541 agcttttttg ttatttagtc ataattttct acttctttat caagttcttt gccacaagaa
    36601 tcacacttat atatagcatt gtatccgtaa tcatattcat caactatttt cttttgtcta
    36661 aatacaccac cacactcaca taggaaacca atttttataa gtgtaacgat aaattgtgat
    36721 tttataaatt ttatatcatt gcatttatcg ttagtgtaaa atgtataaat ataagagtta
    36781 tcgcactcat ctcttaattc aaatctttca atatatgtgt tattttttaa ttcctcaaat
    36841 tcttctttag ttaaatatga tttttgttgc aattcatgta aacgcttaat ttggtccatt
    36901 atttatatcc tcccaataat tttagtattt taagcttaga taaggaatct aagttttatt
    36961 aaatttatcc caaggaatta tttaaatcat atttagtttt attaattaca acatatataa
    37021 attacacgaa aataaaacta attgtaaaaa taatatcctg tgaaaaccta aactactaac
    37081 cccttactaa cccgaaaact aaaaaaagaa aatatatcca aatataagtg atatataaat
    37141 aaagatgttt catataaatt taactttaaa tattctgaca ataatatata taaatatatt
    37201 atagttaaaa tataccacat attttgggta ttgtatactg gaattagagt aattaattta
    37261 ttttttaaca tacaaatgat tttatgaaaa aaaacaagac tcaaaaattt ttatattctt
    37321 tgatagaata aaattgagag taaatttatt gaattttata aaatttataa ggggtgagtc
    37381 taagatatgc atgaagataa aaatgatata tttgaatcaa tagatactga ggattttgat
    37441 aataaaacag aagaagaatg tttagagcta gtaagaaaaa atggattgaa tttaatatac
    37501 ataaaaaatc aaacagaaga gatatgtcta gaggctgtta gacaaaattg taatgcaata
    37561 aaatgtgttc agaatcagac agaaaaaata tgtatagaag cagttaaaca agattggaga
    37621 atgttagaat ttgtggaaga gccaacagaa gaaatttgca tgttggctat taaccaagat
    37681 ggaacagcat taaaatatat agaaaatcag acagaggagt tatgcttaag agccgttgaa
    37741 aaaaatggtg cagctttgga gtatgtaaaa gaacaaacag aagaaatatg tatagaagca
    37801 gttagaaaca gcgaatttgg attggcaaga gtgaaaaatc aaacagaaaa aatatgtatg
    37861 gaagcagtta agcattgtag ctataatcta aaatgggtag aaaatcaaac agaagaaata
    37921 tgtatggaag cagtgagaca aaatgggctt gatttgaaat ttgtaaaaaa tcaaacagaa
    37981 acaatctgtt taagagcagt aagacaaaat ggaatggcat tagaatttgt aaaggaacag
    38041 actgttggta tatgcttaaa ggcagtaaga caatatggaa tggcattaaa atttgtaaag
    38101 gaacaaacag aagaaatttg tacagaagct ataaaacaag ataaaagagc acttagtttt
    38161 gtaaaaggag ataaggaaaa atataaggct ctatatgata ataatgaacc atttgcaaag
    38221 agatatgtta gaaatgtaat agaaaaagaa aacagagcat tgattaaaaa aggagaagaa
    38281 aatgcaaaaa tcaaaattgc aggaaaactt tatgatagag gcatgtcttt tgaagatata
    38341 tcagatatag ttgagattga aataagcaag ttaaaagtga gtttaggtgt tttttaaagt
    38401 aataaatgta ggtgtttttt atgggaaata gttaataaaa aaataatcga ctagctcttt
    38461 gtaggacagc aaaattagtg atataatttg atgtataata aattattttt attagataag
    38521 ggggaattaa agttgaagat tacagtatta taccaaacaa gaagtggaaa cacagaaagg
    38581 gtcgctaagc tgatagaaga gggcgctaaa aaggtggatg gagtagatgt aaagcttatg
    38641 agacttgata atattgattt agattattta aatgaaagta aagcagtaat atttggaaca
    38701 cctacttatt tagcaaatac aacttgggaa gtaaaaaaat ggtttgatga agattcaaaa
    38761 aaagttaatt tagctggaaa attaggagct gtttttgcta catgtgatta tatatgtgga
    38821 ggtccagatg ttgcgatact tactatagta ggacatttga tggttaaagg gatgcttgtg
    38881 tattctggag gtggttcttt aggaaagcca tttatacatt taggacatgt acattcaatt
    38941 gaaggtccag agttacaaga tgagaaggct ataatatttg gtgaaagaat tgctactaag
    39001 gcaaaagaac tatttgctta atttctatat gcatagtaat gattagagta aatttagggt
    39061 tttaatggag atttgaagta aaagttataa aataaaaaat tttccataaa attactcaag
    39121 atgaattata atataattat aaataaatta tattattaaa atatttaaaa tcaaacgtca
    39181 atagcgaggg aagtttatgg tatggcatat agaatatgag attttttctg caattatagt
    39241 tattttttta atggtatact tttttagaag taaatttata cctactttac aaaacaaaat
    39301 atattgtgca cttttaatat tctcattttt atttataatt tcaaatattt taggttcatt
    39361 ttgtctaaat aatatagaca aaattccaat ttttattact tttttattga atcagatata
    39421 cttattgtta ttgccaatac cagcagcact tgtatcattt tatgttatgg ctattattta
    39481 tcaagatata aggtatatga aaaaaagatt attattttta tctataccac ttataatttg
    39541 tataatctta tctattacaa atccttttac gcatattcta tttggattgt caaaagaaac
    39601 aggttatata cgaggttgtg gatatgttgc aacttttgca tcttttttct tttatgcaat
    39661 atattcagca tttttatctt ttaaatatag aaaagcaatg catgaatcaa aaatatgggc
    39721 tattagaggt tttcttatag tttcaattgt ggcaattatc ttacaggcaa tttttttaca
    39781 gtatcttctt acaggaactg catgtgcatg tagtttactt ttgacatatc tttctatgca
    39841 gaatcgaggt cttattattg atgaccttac tggtgtactc aatagacaat cttttattca
    39901 agagctagat ttgaatatca atactgaaca atcaggattt attattacaa ttgcgcttga
    39961 tgattttaag tttatgaatg aaacttttgg aacaaaaaat ggagatattg cactaaaaga
    40021 agttggaaaa tatttgattc agatacatga tgtaaaccat gtatatagaa tagggggaga
    40081 tatattttct attgttgtca atagtcaatt aggtatagaa ccagatgata ttatcagaaa
    40141 gatagaagaa agatttaaaa agccttggct agtagaagag ataagtttta atttagcaac
    40201 tagtattgct gttgtttatt acccagaaaa tgcagaaacg acagaagatg taattgttgc
    40261 tatagatttc tctatacatg aagcaaaacg ctctgagtca aggcgagttg tttatgcaga
    40321 tgcgtctata agtgaaaaga taaagaagaa acatgttatt aaaaattgtt taaaagaagc
    40381 attaaaaaat gatgggtttg aagtgtacta ccaacctata ttttctaata gcaggggcag
    40441 gtttacttca gcagaggcat tacttaggct agaacacaaa gagttaggtt ttatacctcc
    40501 tgatgaattt attccaattg cagaacaaac aggattgatt aactcaattg gattagtagt
    40561 ctttgagaag gtatgtagat ttatagctag tgatgagttt gaagatttaa aattggataa
    40621 tatagctgtg aatctttctg tagtacaatg tatgcaaaaa aacttagctg atgatttact
    40681 ttatcttatg aataaatata atgtatcacc aactaaattt aaacttgaaa ttactgagac
    40741 tgtggctatg ggctctttta atataattaa agaaacaatg gaaagattaa ttgatttagg
    40801 tgttaagttt gcacttgatg attttggaat tgcttattct ggagttacta atatgctttc
    40861 tttaccattt tcagtaatta agttagataa gagtttgatt tggtctatga atgaagatag
    40921 taggcataaa cttacagttg agacgattat agcacttata aataaattga atatgaaggc
    40981 tgtagcagaa ggtgttgaaa ccattaagtg tgctgaagat ttaatagcta tggagtgtga
    41041 atatttgcaa gggtattatt tttctaaacc agttccagaa gatgttttta aaaatttgtt
    41101 gaaagaaaat acttttacaa gttaacaccc tttatttttt taattacaac tttgttcatt
    41161 gacactgcca gcgatatatt ataaaattaa cacaataata aatattaaat ttattataca
    41221 tattattaat attaattaat ggagtaaaga tttattatat attttattta attatcctaa
    41281 agtgagaaag gaagatgtga catgaataaa aagaaaatat tagtgccaat tgatgggaca
    41341 gaaagaagta tgcattcttt ggaatttata aaaggaatat ttaaaaaaga tgaagttgaa
    41401 gtagaaataa tgaatgtaaa agaactagta tttattgatg gcatatccct ggctgaagag
    41461 ataaaaaatt ctgaaaattt aggaagaaga atcttggata aagcagcaga gataatggga
    41521 gagtatgatg ttaaagtaca ttttactttt ggatatccag gagatgagat aattagaaaa
    41581 gcaaaagaag ataatgtaga ttttatagtt atgacaaaat ctactaaaaa aggtcttaca
    41641 agaatgatag gttcagtaac agcaagtgta gttaaacaag caaaatgtat agtgatgatt
    41701 gtaccggagt agaattagaa aggtatttga aatatgaata aaaagatagt agcagtttgt
    41761 gaaagtgaaa gaagtttaaa ttctcttaag acagctgcaa atgatttagg atttgattta
    41821 gtttatgaaa ttcaagaaaa tgataatata aaaaatgaac tttctattta tgatatagaa
    41881 gatgcatcca tagtgttatt tgttacaagt gattctatag aaagcataga gaagattgat
    41941 agatttatag atagagaatt ttatgaagtt gatccaaagt atataataga ggatgcaaaa
    42001 tctatagtaa gtgaaatttt aatagactta aattaatcgt gggtgtcgca ctaagaatag
    42061 agcgacgcct ttttatatat tttacacaag aaataaagag gttgtatcaa aatatagata
    42121 agagtctttt tcattgttga aatgatgaaa atatatttga tttcatacaa aaaagagtac
    42181 ctcataacta aaaagttcac tttgagacac tccattttta tatgttagtt gcatttaagg
    42241 taaaatagaa gtttaatcat gttgaaataa ctgaattaca tcttctaaat gattgtacat
    42301 tgatgtataa cctaagtaag agtctttact atcaaatgca atcattatct tgttatggtg
    42361 gctaatcata tccttgagag aatttctata tttaaaacca aagtattggt tagaagaaac
    42421 tgttttaggt attaactcat aatattttat aaaaattgga ttatgagaag ctaaaattat
    42481 ctctttgtga aattgcaagt cagcatcata acattcttct atcaaaccat gagaaacctt
    42541 atccaattcc tctattgact ttaggtaagt atattttatt ttttgaagtt ctttagatgt
    42601 agcatttaaa attgctagtt tagtagcttc gctttcgata agttgtcttg ctcttaaaga
    42661 ttctacataa aattcttgta tgttatgatt tttatagatt tcaccatcat caaataggtc
    42721 aactttttta aatccttcaa ctataggact tgaataaggc acagctctaa gtattccttc
    42781 cttacagagt atatttatgg catttcttac aggtattcta cttatgttaa ataggtcagc
    42841 aatctttctc tcacttggta atttactatt aattttaaaa gttccattta gaatgtttaa
    42901 tttaatataa tcaataattt tttcagtggc atttaagctt ttcataaaaa ctcctaaaca
    42961 tataattttt ttatgattgt attatatcat agttaagagt taaatctact tttttataat
    43021 atgtataaga caggatagag gccaaaatag ctataatagc caagataaag tatccatatt
    43081 gtattgaagt atattccgat attttaccaa ctatcattgg aaatacagac attcctatag
    43141 cataaattgc ttgaaaaaaa cccattgcag tagatttctg atgttttggt acatttttca
    43201 tagactcgct agtaaggtaa gaaaggagta tacctgtaga tatacctggt agtacttgta
    43261 gtacaaatat tatattaata ttttttattg ttggaactaa aaagcaatag gctgaaacta
    43321 aaataaaaac taaaggaatc caaaattttg gtccatactt agtacaaaat ttagtagaag
    43381 caaagccaga agaagcaaca gcacacacca tgtaaaatat agaagataga cctatgaggg
    43441 tagaactaga acctaagttt ttaagaattt gagtagtaaa tgacattgtt gtagacattt
    43501 gaataccctg ttgaattaat gccataaagg aaaaaaatac aagtctttta tccttgcaga
    43561 tagataaata ttttttaatt gatatttttt caggtatttt tttagaatta tgatttttta
    43621 tatttaaggc tagaatcgtt ccaataactg aagacaatac actcatgata cataaggctg
    43681 tcattcctaa tttttcataa aataaagtac tcataacaaa tccaatcaac attcctagat
    43741 tgtttaccaa tattatcgta cttgtagctt tttgttgatg ttctgtagaa aaaaagttgg
    43801 tatagtgaac catgtaagaa atccaagttg atgatgatat tccagataaa atgttagcta
    43861 taagaaaacc attgcctcca ggagaaaaga ctcttataag agatgctaaa ccagcaaaga
    43921 aaattccaga cagtataaat attttatgtt tatttatgga atcagcaaat actccaacag
    43981 gaagccttaa taaacattgt gatattccat atgcacctat tattatacca atcatatttg
    44041 tagatacatt acatgcactt aaatatggat tttggaaagg tatatataca tattgtgaaa
    44101 accagaaaaa tgtaacaata gaaagtaaaa gtaatttttg attagatatt tttttcatat
    44161 tatcctccat gtggtatttt tataatgcaa ataatatacc atttagattt tttaatcaag
    44221 tactttctct attaaaaaaa atttaaatat ataattatta tttacaaaaa aattatactt
    44281 tatagttgaa ttaagaaaac gcttatgata ctataaagtt aacaaatttg aatatataac
    44341 agtagacgtg ttactggtaa tgcaggcaag atcgaatcaa tttttataga ttatctatag
    44401 agtactatgg gtataatcta tttatttggt tcgatttttt tgtttaattg gccataaaac
    44461 aaaaagccta tagttgaaaa tggaggattt tttatggatt ataaaaatat agctcaagaa
    44521 attctactaa atgtaggagg aaaagagaat gtaaatgaag tgactcattg tatgacaaga
    44581 cttagattta aagtcaaaag cgcttctaaa gtgaataagg ataaattatc taaaactgaa
    44641 ggagtaataa ctgttgttga gagtatgggt caaatacaag ttgtaattgg aaataaagta
    44701 aaaaaagtat atgatgaggt cataaaaatt gtacctcaat caaataacat aggtaaaaag
    44761 aatgaaagtc aagaaaagca aggtataatt aattctatat tatcagcagt agctggtata
    44821 tttacaccta caatacctgc tatagcaggg gttggtatga ttaaaggtat tttatcagtt
    44881 cttgctatgt attatatgaa taaaaatgga gtagatataa aagaaacgca aagctatatt
    44941 atcttaaatg ctatggctga ttctatattc tattttatgc ctattatact aggatatact
    45001 gcggctaaag tatttaatgc aaataaaata atatctatgg tattaggagc tacactttgt
    45061 tatccaactt ttactgcttt gatggcaggt gaagaatctg ttagattttt aggattggca
    45121 gtaacaaaag ctacgtatac atcatctgtt attccaataa ttattgctat atgggctctt
    45181 tcatacgttg aaaaagtatt agaaaagtat atacctgagg taattaaaat aataatggtt
    45241 ccaactttat cattggtagt aatgttacct gcaacattat ttttatttgg accaatcgga
    45301 atttatatag gaaatgtcat aaacttttct tataagtata tttatgaatt aagcccagca
    45361 ttgtgtggag catttgtagg tgggttatgg tgtgtgttag ttatatttgg tgctcataga
    45421 gcattattac caataggtat aagtgatgtt gctcaaacag gaagacaaaa tttattggcg
    45481 tttgcaggag ctgcaaactt ttcacaagca ggggctgctc taggagtatt ctttaaaact
    45541 aaaaatcaag gactaaaaac aatttctatg tctgcaacta taacagcttt atttggtata
    45601 actgaacctg caatatacgg tgccaatctt agattaaaaa aacctatggt atgtgccgta
    45661 atatgtggtg catttggagg tgcgataatg ggaatgggtg gagcttatgg taatgcattt
    45721 gcaaaccaag gtgttttaac tatacctgta tatgctgaag caggtgcatt aggtttctta
    45781 agttatctag gtggttgtgc tatagcattt tttggttcag ctatttcaac ttatctagtt
    45841 ggatttgaag atttggaaga gtcttctaat aaggaaaatt catctataaa agtagaaacg
    45901 aaggatggtg ttattgatat aacttcacct gtagagggcg aatgtataga attgtcagaa
    45961 gtaaaggatg atgtatttgc ttcaaaagct atgggagaag gtatagctgt gttacctaca
    46021 aaaggagtaa taacagcccc tactgattgt gaagtagctt ctttatttcc aacattacat
    46081 gcaataggat taaaattaga taatggagca gaaatgttaa tacatgttgg aatcaataca
    46141 gtagagttaa atggtaaata ctttacaaaa catgttaatc aaggagattt agtgaaaaaa
    46201 ggtgataaac ttatatcgtt tgatatagat aaaataaaaa aggctggtta tgatgtaact
    46261 acgccagtta tagtaaacaa tacatttgat tttgggcaag tagtttcttg taaatctagt
    46321 tatgtttcaa ccaatgataa tataatttca ttagtattag ctggtaatta agttaatttg
    46381 actagagata agtaaattga tttaaaagga aataagtaaa tcaacaatta aatacatcta
    46441 aaagtaagtt tattgcaatg aattgttaaa gaaaataatt aatatggagt aaatctattg
    46501 caatgaagta acaaagaaaa taattaatat agagtaagtt tattgtaatg aattgttaaa
    46561 gaaaagagtt aatatagagt aagtttattg taatgaattg ttaaagaaaa taattaatat
    46621 agggtaaatc tatctcaatg aaataacaaa gaaaagagtt aatatagagt gaacttattg
    46681 caatgaatca acaaagaaaa taatcaatat aaaaaggaga aatatttatg aatacaggat
    46741 ttccaaaaga ctttttatgg ggagcatcat cttctgcatt tcaagtggaa ggagcttggg
    46801 ataaagacaa taaaggaaaa accgtagccg actataattc ttttaaaaag tctcatttac
    46861 aggcagatac taaagtcgca tcagactttt accataatta tgaagaagat attgaattaa
    46921 tgaaagaatt agggatgaaa acatatcgtt tttcaatttc atgggcaaga ataataccag
    46981 atggtgaagg agagataaac cagaaaggtt tagaatttta taataagata atagacaaac
    47041 tgatagagtg tgatattgag ccattcgtaa ctttatacca ttttgacctt ccatttaaat
    47101 tagtagagaa atataatggt tgggaaagta gagaaactgt ctatgctttt gaaagatttg
    47161 ctaaaatatg ctttaagcat tttggagata gggtaaagta ttggcaacct cataatgaac
    47221 agaatttaat tgttagagtg gaggagagaa ttaatatata tgatgaaaca gattcatgga
    47281 agatagataa aatgcgtgct cagatggatt ataatctttg cttagcacat gcattagcag
    47341 tcaatgcatg tcatgagatg ataaaagaaa gtaaaattgg agcagcagta tcatcatctg
    47401 taacatatcc tcttacttct aagccagaag atgtatatgc agctagaatg aatgataatt
    47461 ttaaagtgta ctatatgctt gatatgcatc attatggaga atatccaggg tattatatga
    47521 agtatcttga aaaaagaaat atagttcctc acatggaaga tggggataaa gagatactta
    47581 aaaaagctaa aatggatttt atagctgtaa attattatag gacaaattgt gcagaagctt
    47641 tacctgaaga ttcacaacat ccatttggat taagagaagg aactgttgat tttagtatgt
    47701 atggactatt taaaatgtca atgaatccta acttagaggc ttctgagtat ggagcagcaa
    47761 tagacccatc aggtcttaga gttgccttaa atgaatattg gcaaagatat cacttacctg
    47821 ttataataac agaaaatggt ctaggagcta aagatatatt agaagatgga aaaattcatg
    47881 atgactatcg tatagactat ttaagaagtc atataaatgc atgtaaacta gctattgaag
    47941 atggtgtaga gatgattgga tattgtccat ggtcatttac agacctttta agttcttctc
    48001 aaggttttaa taagcgttat ggacttgttt atataaatag aactgaccat gaagttttag
    48061 atttaaaaag aatcaagaaa gatagttttt actggtataa agaagttata gagaacaatg
    48121 gaatagtaaa ataaacataa atatatattg agggttttag tatgtacatt acaaaggtat
    48181 taaataattc gttgctactt gcaaaggatg ataatgatga agaaataata ttgatgggga
    48241 aaggcattgg ccataattat aaggccgggt ctgagttaaa taaaaaagat atagaaaaga
    48301 tttttgttct tcatgatgaa aatataaaaa agagtattat acaattggca acagaaatag
    48361 atgcagaata ttttggtata gctcaaatga taattgcata tggaatagaa aaatataact
    48421 taaaacttat gaaccatata tatttggcct taactgacca cattgccttt gcagtgagac
    48481 gttttaaaga aggtataatg attgaaaatc actatttgtt cgaaatcaag gaatttaatc
    48541 caaaagagta tgatatagga aaatatgcta ttactgtttt taaggaggtt cttggtttag
    48601 acctaccaga agaagagatt ggtaatatag caggccattt tataaatgca caacaagata
    48661 atccttatag tgatagaaat aagagaagtg ctaaaattgt aaatgctatt ttgcaaatag
    48721 ttcattatca cttttctatt gtatataata aagagtcatt ttattatagg agatttgtaa
    48781 tgcatctaaa ggccttttca caaagatttc tggctaatga accatcaaga gaaaaaatag
    48841 attttatata tgagcaagtt caaaaaaatt gtaaggaaga gtatgaatgt gtgaaaaagg
    48901 taggagctta tatgtataag gaatactcca gagaattacc aagacaagag gagttatatt
    48961 tgatgattca tattcataag attttaggag aattatgcga aaatagttag aatatcttaa
    49021 tatgctagta atattataaa aaattaaaaa ttaactgcac tttaaatatg aataattgca
    49081 ttacttaagg gggttgtctt aaaattgatt tttttaatta aatttgaagc tcaactcctt
    49141 ttttaaacct ttatatcatt acaactttaa tgtttatacg actgtacttt agatgtatat
    49201 ctacttttta catttctcaa catgcaaacc taccattttt tgcagtatgt taattgaaat
    49261 aaaccattga gttaacccta aatttattct ggacttatag gaatagttcc aaagatactt
    49321 taaaatattt tctggaataa ttgcttattt cacatatgtt ttattgtgat gttttgattt
    49381 aataaattct tcaactctgt atatagtaaa tgtattatca tgtttgtttt attcaagcac
    49441 acttataata cgcttgattt tttcacaatg atgaaatata ctaatgggtg ttataggtat
    49501 aagaatcgca ttacacttga agaagtaagt gataaatgag gtataaatcc tcatatgata
    49561 aactactatt tcattgagga acatattcta agtgatgtca agatactaca atatgactaa
    49621 attcctagtg ataaaaaagt ctacgaatga gtgtagaaag gggaactaag atgaataaag
    49681 ttttaattat agatgatgat aaagatcttt gtattttgtt aaaaaagagt atttcaattg
    49741 aacagattga agcaaactat tgccattctg ggagagatgg actggttgaa ctttcaaaac
    49801 aagattatca gttagtagtt ctagatgtga tgatgccagg tatggatggt tttgaaacta
    49861 tggaacaaat acgaaaatat agcagtattc ccatcttaat gctgacatca aaaagcgata
    49921 attacagcaa agtgcatgga ttgcgagctg gtgcagatga ctatctaacc aaaccatttg
    49981 aaattacaga atttactgct cgtgtactgt ctttaatccg acgttacacc cgttttaatc
    50041 caaaaaatga ggagccacag caactttctt tcaaaggttt gttgattgac cttgaccagc
    50101 atacagttac taccaatggc gaacctatgt tgcttccacc caaggaattt ggtgtacttc
    50161 tttactgtgc aaggcatcag ggtaagattt tgacaaaaca gcagatttat gaagccgtgt
    50221 ggggtgagcc atatgtatat gatgacagca atatcatggc tattatcagt cgcctaagaa
    50281 aaaaaattga actagaccca tcatctccca tctatttgca aacaataaaa ggaattgggt
    50341 atcaattcaa cacggaggta tgaaaatgga tgaacaaacg ataattcttt tcttactctt
    50401 gctgtcgatt ggtctacttt ttctttccat atttgctttt tccaagcttg ggcatattta
    50461 caagcgccta aaagatatag aggagatact ggctgatgta caaattggag acgaaaatcg
    50521 taaaatacta attaagccat gtgatgttat ggctccattg gtttatcagc ttaatgagat
    50581 tgtctacgat tatgaaaata agttactttc tctgaaaaag tcggataaag ctagcaaaca
    50641 acttatgaca agtttatctc atgatgttcg tacaccatta acaactctga ttggttatct
    50701 ggatgctgta cacagtggta tagttatcga acaagagcgg gaggaatacc ttgaaatcgc
    50761 taggcgtaga gcctacgacc tcaaggatta cattgatgtt ctttttgact ggtttcggtt
    50821 gaactctgat gaatttacgc tgtcaataga atctgtggaa attgcagagt tgtcacgcaa
    50881 tattttgaaa gactggattc ctatattcca tgaaaagaaa ctgaattttg aaattgatat
    50941 tccagaaaat cgactaatgg taaacctcga cccagatgga tattcccgtg tggtcaataa
    51001 tctggttcaa aatgttcttg ctcacagtaa ggctcggcaa atcaagatta ccatgagtga
    51061 ggattccaga atggtattac tccgtgttga ggatgatggt gttgggattg ccagagaaaa
    51121 tttaccacat atttttgagc gactgtataa gtgtgataaa ggacgttcag aaaaaggaag
    51181 tggtcttggt ctatctattg tcagtcaaat ggtggagcga atgggtggtc aggtttccgt
    51241 agaaagtgag attggaaagt ataccgtatt tacagtgagc ttaccattaa tataaaattg
    51301 tttttccccc atgtgacatg tatacacatg gggatttttt gtaagttata aagttgcaag
    51361 gttaatacaa ggttgttgca aggttaatgt aagattgagg tggtatgata actttaataa
    51421 acaaaggagg cttttattat ttatgaataa tttaattatt gaaacaaaaa atctcactaa
    51481 gcaatatggt gctcaaaaga gcgtttctaa tttgaatatt cacgttcaga aaggtcgcat
    51541 ttacggattg ctaggaagaa acggtgcggg taagacgaca actatgaaaa tgttattggg
    51601 tctaacaaaa ccaactactg gagaaattaa aatatttggt caggacattc acaataacga
    51661 gaagaagctt cttcctcgaa ttggatgttt gattgaatca ccagggtttt atcctaatct
    51721 gacaggaaca gaaaatctca aaatctttgc aaggcttaga ggaataccaa aaaaaaatgc
    51781 agtagaatca gcactggatg tagtcggttt gccaaacaat gacaaaaagt tattttctca
    51841 atactctctt ggtatgaaac agaggttggc gattgctctt gccattatgc atgacccaca
    51901 actgttgatt ttggacgagc ctatcaatgg acttgatcct attggtattg cagaagtaag
    51961 aacgtttatt cgtgatctct gcacagaacg aggtaaaact attttgattt ccagtcacat
    52021 tctttctgaa attgccttgt tggcagatga cattggcatt attgaccatg gtattttgtt
    52081 ggaggaagaa agtcttgccc aattagaaga aaaaaacagt aaatacatac attttgctgt
    52141 atctgatgca gcacaggcag caaggcttct ggaaacccaa cttaacctga aaaatttcaa
    52201 agtagcagat aattacaata ttatgctata tgatacagat gtttctgttg cagaaataaa
    52261 tcgtgctttc attctaaaca acattgaggt atcagaatct catttatgcg aagatactct
    52321 tgaagattac ttcaagaaag taacaggggg tgaaggaatt gcttgatata atttcatgtg
    52381 aattttcaaa actcaagcgg ttaaaattta tactgatttc tattttaggt gcttgcttat
    52441 ttccaattcc cgcaacaatt ttgattgcaa aggacaactt gccctttgag caattgttca
    52501 aattagttgt caattttgga tattttttgc tattaccaat tgtattaagt attgttgcta
    52561 gccaactatt ttttatagaa agagataatg atgtattaaa aaatcttgca acagtgccag
    52621 tgcaaaaagg aaaactggct ctggcaaaat tggctgtgtt gctgtttata tcattgttct
    52681 actctgttgc agggttagga gctactatca ttggaggttt cattgttgga atagtagagg
    52741 gggttgctgt aaaattagga atgagcattg cttttggaat tatgtttttt gttactgtac
    52801 ttcctgttgt ggtactcatt gtatatttca atcgcagcta tattttttct attatacttt
    52861 cttttgtata tgctattttc aattttagta tatccttaaa tattatcaat tttgaaccaa
    52921 ataacccaat attaagtgta ttacctgctc ctgttataat gcgttggtgg atggcatttt
    52981 ggggagatcc cactggcgaa tacacggcac ttagacagcc atatttgctc tcaactcctg
    53041 cttgtgtagg tattttatgt ttgataacca ttatagcagt cctgctgatt tgtatgatat
    53101 acaaaaagca ggagaattag gaggtatcta tgcttgatat aattactaca gaatttcaaa
    53161 aaataaaaag atataatatt ctatggattg gtgttgttgc tgttctgttt tcagcactat
    53221 tggcagtttt tcaggttgta agttcacacg gcagtgaccc tctaacgtat gagagctttg
    53281 caaacggagt tatctggaat aatttcagtc ttagttttcc atttgggatt acacttattg
    53341 gaggatatct tattaaccgt gaatatacag accaaacctt aaaaaacatc ctaactgttc
    53401 ctatttcatt acgcaaattg ctagtgggaa aattaattgc ggttggagga attactgtat
    53461 tgttaagtct tttcagcttc ttatgcacat ttattttggg gttgttgttt tgcaatatca
    53521 atatatctgt tacactcgtt gtaaaatcct tagttcagat tttgtctgtt aatctgtatt
    53581 gttatgttgc tgttttgccg attattgctt attttggtcg taaacaaaat gcatttctga
    53641 cgggtgtagg gattgcattt gtatacggtt tttgtggtgt gtttgttgct ggaagaaatc
    53701 tgacagactt ttatccaatc acagcaggtc ttggtctagt tggatacaac aatggtgctg
    53761 gtactgttta tcagccactt atcggtgcta tgactttgat tgtaattcta atcttgacaa
    53821 ctattctact tgtatttaca cctaattatg ataaagttat gaccatatct aagaaaaaag
    53881 ggataaagca taaaaagtat tctcataaat aacataaata ttgtttctat tatttaagtt
    53941 gctagacagt gaaaagaaaa aatctgtcgt acagtgtcta tatgctcatg tattcaagaa
    54001 gctacagttt tgatttttca taactgtagc ttctttttgt atttattaaa tcacgactat
    54061 cataatactt gcaataggta tagcgacaag agagagtact gtcatatgtg ttttatatat
    54121 tacattaagt atattctaaa atatatgttt taaataatat atttgaaaat aggaatattt
    54181 aaataaaaaa aataatatta tatatagttg acaaaaattg gcaaatgaga taatatataa
    54241 atatagaaat agtaatcatt actattatta aaaagggagg aattaattat gaacttaaaa
    54301 ggaactaaaa cagaaaagaa tttaatggca ggatttgcag gagagtcaga agcaagaaat
    54361 aaatatacat actacgcttc aaaagcaaga aaagaaggtt ataatcaaat agctgctata
    54421 tttgaagaaa ctgctaacaa tgaaaaggaa catgctaaat tatggtttaa acttttacat
    54481 gatggtatgc cttcaacaga ggaaaattta aaggatgcag cagcaggtga aaactatgaa
    54541 tggactgata tgtatgcaaa atttgctaaa gaagctagag aagaaggatt tgacaagata
    54601 gcttatttat ttgaagctgt tggaaaaata gagaaagagc atgaagaaag atacttaaag
    54661 cttttagaaa acttaaatga aggaaaaata tttaaaagag atgaagaagt tgtttggcaa
    54721 tgtcaaaact gtggtcatgt gtatgtaggt actgaagcac cagaaaaatg cccagtatgt
    54781 gaccatccaa aagcgtattt caatataaaa gctgaaaatt attaaaaatt atcattatta
    54841 aaaaggaatg ttaactatga aattttctaa acaacgagaa ctgattttaa atgaaatatt
    54901 aaataatccg gttcatccta ctgcggatta cctatatgaa aacttaaaaa aagataatcc
    54961 aaatttaagc ttaggaactg tgtatagaaa tttagctcag ctaacggaac atggctttat
    55021 aagaaaggtt agtattccag ggtatccaga tagatttgat ggtcgaatag ataatcacta
    55081 tcatatcata tgtgaagtat gtggagaggt atacgattta gaatccgaag ttcttaataa
    55141 tttacaagaa ttaatatctg aggagacaga cataaaaata acatcttata acataagttt
    55201 taaagggatt tgtaataatt gtaaaaggtg tagccaagta ggttagtttt caaattctaa
    55261 aaataattaa tgaggtgagt ttatgtgtag tgaacaaaaa ttttttatat gtgagacttg
    55321 tggaaacctt gtaggaatga tacaaagtgg tggtgtacct attttctgtt gtggaaaacc
    55381 tatgaaggaa cttgttccta atacaacaga tgcagcagtt gaaaaacatg tacctgtaat
    55441 tgaagtggat ggaaataatg ttactgttaa agtaagtagc acaactcatc caatgactaa
    55501 agaacatcat atagcatggg tgtatcttat gactgaacaa ggtggtcaac gcaaatgtct
    55561 agcagtagat ggagaacctg tagttaaatt tgctttgaat gatgatgata aagtgatttc
    55621 tgcctatgct tactgtaatt tacatggatt atggaaggct gaactgtaat atttaaataa
    55681 attgagtcaa aaaggaggct ataaatgtat tttcatacaa atagcctcct ataaatttat
    55741 ataaattcaa gttttaattt tgtttaatgt caatataata taaggagaaa ataatatgtc
    55801 aatttataaa tgtagtgttt gtggatatat ttatgatgaa tctaagaatg ataaaacatg
    55861 ggatgaatta agtgaagatt gggaatgccc tgtatgtaca aaaggtcgta gttattttgg
    55921 aaagataagt actgtttact atgaagaaga tgaaaagata gcagaagaca tagttgaaga
    55981 tgaatctaaa ttaaatactg aaaaagaggg agatttaaat tacttaagta catatttaag
    56041 aagagatgat gaagttgaga aacatatgga tataattcac gaaatggctg ttacaggaaa
    56101 atctataata gaacctatga gaactaaact accagtaatt tcttgggatg atattttaat
    56161 tatgggtgct caattaaatc cattaccttt aaatgagcat gatgaagtaa atacaacaac
    56221 aataatagga aaaaaagcaa agaaacctat gattatagaa aatccagttt atatttccca
    56281 catgtcattt ggagctttgt ctaaagaact aaaaatagct ttagcaaagg gtgcagccca
    56341 aaataagaca gctatgtgta gtggtgaagg aggtatatta ccagaagaga aggaagcttc
    56401 atacaagtat atttttgagt atgtaccaaa taaatatagt gtaactgaag aaaatcttaa
    56461 aaattctgat gctatagaga taaaaattgg tcaaggaaca aaacctggta tgggaggtca
    56521 tcttcctggc gaaaaagtaa ctgaagaaat agcaaaagta agaaataagc ctgtaggtca
    56581 agatgtaata agtccatctt gttttgagga aatacaatcc aaagaagatt taaagaaact
    56641 tgtagatgaa cttagagaag tttcagaagg gcgtccaata ggtgttaaaa tttcagcagg
    56701 tcatattgaa aaagatatgg aatttatagc ttatgctaaa cctgattttg taactattga
    56761 tggtcgtggt ggagctactg gagcaagtcc aaagttgctt aaagatgcaa cttcaattcc
    56821 aacaatattt gctctttatc gtgctagaaa atacatagat acacatggat tagatataga
    56881 tttggttata acaggtgggc ttcgtatatc tactgatttt gctaaggcaa ttgctatggg
    56941 tgctgatgca gttgctattg caagttctgc tcttatggca gcggcgtgtc aacaatatcg
    57001 tatatgtggt tctggtaagt gtcctgtagg agtagctact caggatgagg aattgagaaa
    57061 aagattacat atagaaaaca gtgctaatag agtggcaaac tttttaaatg taagtttaga
    57121 agaacttaag acttttgcta gaatttctgg tcataaagat atacatgatt taagtgttga
    57181 tgatttatat actgttaact ctgaaatatc aaattataca aacatacagc atgtatagat
    57241 tgaagtttga aactattaga ttttagatag ctataaaagt aaatgataaa tatattttat
    57301 aaaagagagt gtcttaaaat gaacttttta gttcataagt cactcttttt tttatgaagc
    57361 caaatatatt tatcatttta gcaatgaaaa agaatcttat ctctttattt tgagatagac
    57421 tccttctatt tatgaaaatt aaagatatta aattgtaaaa tattctgtga tgtcgtacta
    57481 ttaaactaaa gattctgtac tttaaagtaa ataaaaattt aaggagtgat tattatggaa
    57541 agattgaaga tacaagtttt agttgacaat aatacttata ttgatagata ctttgttgga
    57601 gaaccagcag taagctatta tattgagatt gatggaaata ggattttgtt tgactcaggg
    57661 tattcagatg tattcatatc aaacgctgaa aaattaaata tagatttagg aaatttaact
    57721 catgttgttt tttctcatgg gcataatgac catacaagag gtatccagtt tctagaaaat
    57781 aggtatgatt tatctacagt tgaacttatt tcacatccta actgttttat acctagaaaa
    57841 catggagaga aaagcatagg agctccattt tctgctgagg aaattaaaaa tatttttatg
    57901 tataatccta aggataagcc atttaatcta agtaagaatt gtgtgttttt aggtgaaata
    57961 ccttctataa atgattttga aaaaagagct aaaattggta aatgtaaaat aggagatttg
    58021 tgggaagatg attatgtttt agatgattct gctatagttt gtaaaactga taaaggtatt
    58081 tttattgtta caggatgttc tcatagtgga atttgtaata tagtagagta tgctaaaaag
    58141 gtatgtggag atgatagagt tattggtata ctaggtggtt ttcatctttt tgaattgaat
    58201 aataggttag atagtacaat acaatatttg gataaagaag aaatgagaat gttgtaccct
    58261 tgtcactgtg tttcttttaa ggtaaaagcg aagatgaatg aaatattgaa tataaatgaa
    58321 gttggagtag ggctgaccat aagtatatag tatttgaaag tatataagat ttaagttgaa
    58381 ggttaaaaag atattttcta tagaaaatat cttttttgtt acgt
//
LOCUS       DAMJYR010000008        58192 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.008, whole genome shotgun sequence.
ACCESSION   DAMJYR010000008 DAMJYR010000000
VERSION     DAMJYR010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 58192)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 58192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..58192
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.008"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(130..324)
                     /locus_tag="PC471_000506"
     CDS             complement(130..324)
                     /locus_tag="PC471_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2787647.1"
                     /translation="MFQVINFLCLFCVGFLVLDVMIYFCKDAMDIFEGTTNVNKVKSI
                     NVWADRVRANSYSYENKIAK"
     gene            complement(633..2162)
                     /gene="lysS"
                     /locus_tag="PC471_000507"
     CDS             complement(633..2162)
                     /gene="lysS"
                     /locus_tag="PC471_000507"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HDF2787648.1"
                     /translation="MKNNQQSNEEAQIQEDLSEVLQVRRDKLKKLQESGRDPFKESRY
                     DRTHYSMDIKDNFDSLEGKTTKIAGRIMSKRIQGKAGFIDIQDQEGRIQSYVRLDAIG
                     EEEYSVFSTYDIGDIVGIEGEIFKTKKGEISVKAKSVVLLCKSLQVLPEKYHGLKDQE
                     LRYRQRYVDLIVNPEVKNAFLIRTKALKALRAYLDDRGFLEVETPILNTIAGGANARP
                     FITNHNTLHIPMYLRIANELYLKRLIVGGFDKVYEMGRMFRNEGMDLKHNPEYTAIEL
                     YQAYADYTDMMEITENVIAHMAEVATGSMIVNYQGTEINFTPPWKRMSMEDCVKEYSG
                     VDFSTINTDEEALEVAREKGIEIKPGMRRGEVINAFFEEFGEDKLIQPTFITHHPVEV
                     SPLSKRNVEDPRRTDRFEAFANKWELANAFSELNDPIDQKGRFIDQLRKRELGDDEAF
                     EMDEDFLKALEVGLPPTGGLGIGIDRVIMLLTNSPSIRDVLLFPTMKPIDNNPNKEEE
                     N"
     gene            complement(2177..2656)
                     /gene="greA"
                     /locus_tag="PC471_000508"
     CDS             complement(2177..2656)
                     /gene="greA"
                     /locus_tag="PC471_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HDF2787649.1"
                     /translation="MEENKEFLLTQEGYDKLEEELENLKVVKRKEVAERIKVAISFGD
                     LSENAEYDEAKKEQAQVEERILKLENMVRKAVIIDESKIDLNVVTIGSIVKVKDLEFD
                     EEVEYTIVGSTEADPYDGKISNESPVGKALLGRAAKEVVEVQVPDGVAKFEILEIRR"
     gene            complement(2765..3733)
                     /gene="dusB"
                     /locus_tag="PC471_000509"
     CDS             complement(2765..3733)
                     /gene="dusB"
                     /locus_tag="PC471_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HDF2787650.1"
                     /translation="MKIGNLELKNKVFLSPMAGVTDLPFRLICKEQGCGLLYTEMING
                     KALCYDDENTKKMLKIEEEEHPVAVQIFGSEPEFMGRATEIMNDYSNEILDINMGCPA
                     PKVVKNGDGSALMKNPKLAEEVLRAVVKNSKKPVTLKIRKGWDDNSVNAVEIAKIAED
                     CGISALAIHGRTREQFYTGKADWDIIAEIKKNLSIPVIGNGDVFTIEDSINMLDKTGC
                     DAIMIGRGAQGNPWIFKRINHYMNTGEILPEPTLNEKISTAIKHLKLAVEEHGEYVAV
                     REMRKHIAWYLKGLRNSAKLRDEINKIEDYQEVVSKLEYYMEDSLT"
     gene            complement(3870..4640)
                     /locus_tag="PC471_000510"
     CDS             complement(3870..4640)
                     /locus_tag="PC471_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HDF2787651.1"
                     /translation="MLLVFDVGNTNMVLGIYKGDKLVNYWRIKTDREKTSDEYGILIS
                     NLFDYDNVNISDIDDVIISSVVPNVMHSLENFCIKYCKKQPLIVGPGIKTGLNIKYDN
                     PKQVGADRIVNAVAGIEKYGAPSILVDFGTATTFCAISEKGEYLGGTIAPGIKISSEA
                     LFQSASKLPRVELAKPGMTICKSTVSAMQSGIIYGYVGLVDKIISMMKKELNCDDVKV
                     IATGGLAKLIASETKSIDYVDGFLTLEGLRIIYEKNQE"
     gene            complement(4659..5249)
                     /locus_tag="PC471_000511"
     CDS             complement(4659..5249)
                     /locus_tag="PC471_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HDF2787652.1"
                     /translation="MMNTSASVRKVNVRKMTIIGVLSAISIMLSMTPLGFIPVGPTKA
                     TIMHIPVIIGAIMEGPIVGAAIGFIFGISSLLNAITNPTATSFVFINPLVSILPRVMI
                     GVLAYYVYQLIIKATNKVYISGLITGAIGSLLNTAGVLGMIYVLYADKYLHAMGKSGS
                     AGKVIMALAAANGVPEAIVGALVVAAVATVLKKSKK"
     gene            complement(5432..6400)
                     /locus_tag="PC471_000512"
     CDS             complement(5432..6400)
                     /locus_tag="PC471_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HDF2787653.1"
                     /translation="MYNNILDIEGLKVGQVEDRNGLTGCTVVICEEGATCGVDVRGSA
                     PGTRETDLLDPVNMVQKVHAVVLAGGSAYGLESTCGVSRYLEEKGIGFDVGVGKVPIV
                     VGAVLFDLGVGSFKCRPDLKMGYRACEVANTDILNQGNYGAGCGATVGKIRGQEYAMK
                     GGIGSYSIKLDNGLIVSALIAVNALGDVYENGKVIAGALNDKKTETIDTYKLMKQGES
                     KGGFSVDNTTIGIIATNAKLTKAGCKKISQMGHDGYAKSIFPIHTPHDGDTIFTISTG
                     EIEADITLVGSLAVEVVEKSIINAVKKADSVENIMAYKDIVKKREI"
     gene            complement(6393..7370)
                     /locus_tag="PC471_000513"
     CDS             complement(6393..7370)
                     /locus_tag="PC471_000513"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HDF2787654.1"
                     /translation="MRDKIIEIILNNKSEFISGEELSKQLGVSRAAIWKHMKALKEEG
                     YNIESVNKKGYRLAENPTDLLSPQNIYYKLDTEFIGKNIEHLDTIDSTNEHAKRIAIN
                     SVDGTVILSEHQDMGKGRLGRRWDSKPHEGIWMSIILKPDMEPFKAPFITLIAGASVT
                     KALNNLGANTLIKWPNDVILNGKKICGILTELSAEIERTNYVVLGIGINVKIMDFEQE
                     IADKATSLYKEGYMLSRVDIVRNVLTEFEKLYLDYTVNNSKEKTLKICRDYSAIIDKN
                     IYVLKGDNRELVKCLDINEEGNLLVQRSDGSIKEIISGEVSIRGEKGYV"
     gene            complement(7685..9655)
                     /gene="ftsH"
                     /locus_tag="PC471_000514"
     CDS             complement(7685..9655)
                     /gene="ftsH"
                     /locus_tag="PC471_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HDF2787655.1"
                     /translation="MNKLLKGAGFYLLVFIIIVGIVQFSGKPTEKIKDLKFSEVYREL
                     TDENISRLYFVNQTSVEGTIKDTNTKFKSYVPTEVMGNKLADEVLDQAKAGKLTFGGE
                     AKPSTPWFVEMLPTLLLIFFMVIIWFVFMNQSQGGGGKVMSFGKSKAKVHKDDEKTRV
                     TFKDVAGLDEEKEDLQEVVDFLKNPKKYIELGARIPKGMLMVGPPGTGKTYLSRAVAG
                     EAGVPFFSISGSDFVEMFVGVGASRVRDLFEQAKKSAPAIIFIDEIDAVGRKRGAGLG
                     GGHDEREQTLNQLLVEMDGFGVNQGIIIMAATNRPDILDPALLRPGRFDRQVVVGTPD
                     VKGREAIFKVHSRNKPLSDDVKMDVLARRTPGFTPADIENLMNEAAILTARKREKKIK
                     METIEEAITKVIAGVAKKSKVISEKERRLTAYHEGGHAVCAHVLEEVSPVHQVTIVPR
                     GRAGGFTMQLPVEDKFYATKNEMKENIIVLLGGRVAEELVLKDVSTGASNDLERVTAT
                     ARSMVTKYGMSSKLGPMSFDSDDEVFLGNSFSSKRNYSEEVAFEIDQETKRIVDGAYD
                     KTRSILQENMDRLEYVAQALLIYETLDAEQFVKAFNKELPLNEIENAVTEENSSKEVE
                     EQLTIKLEKDEEERNNVIDINKNLEDKSDKDK"
     gene            complement(9689..11077)
                     /gene="tilS"
                     /locus_tag="PC471_000515"
     CDS             complement(9689..11077)
                     /gene="tilS"
                     /locus_tag="PC471_000515"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HDF2787656.1"
                     /translation="MIFDKVLSTINKHNLIQKGDKIVLGLSGGPDSVCLLHVLNRLKK
                     DFNIEIYAAHLNHQIRGIEAQKDALYVSKLCEDMGIIFFVKSINVPKYCENEGLSLEE
                     GARKLRYEMFYEIKDKIKANKIAIGHNLNDQAETVMMRIMRGTGLKGLKGIDYIRDNC
                     IIRPILDVERNEIEEYCEAYNLNPRIDKTNLENIYTRNKIRLDLLPYMKDNFNSNVIE
                     SIVRMSNSLKSDNDYIEKEAEAKFREVSNIKEKGFVEINLDDFVCLHDAIKVRVLRNS
                     IKHILGDTNFVDQRHIEDIMSLEDNSKVNKMLTLPRNIFVYRKKDSIILTNEEIVNEE
                     IEFYYNVPSNGFIKIKELKQIIETQVMSIDRYKSMKLDNSSKGFDFNKVKGGIVIRSR
                     RQGDKIKLAMGSKKVKDLFIDLKIPREERCKIPIITDGEGIICVGDYKISENYKIDES
                     TKEVLKINFNKL"
     gene            complement(11260..11688)
                     /locus_tag="PC471_000516"
     CDS             complement(11260..11688)
                     /locus_tag="PC471_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HDF2787657.1"
                     /translation="MEEKSLSVKISINTRQYDEKGNMDTIEMTAFGKIFYKNDGIYVI
                     YKEKEEDIEITNTIKILKNQVSIKKFGAINSTMLFKCGESSTTKYVTPQGTLLIDIDT
                     RELDINIQEGEHIKLKIDYNIKVQDLFLGRNKIDIYIGTK"
     gene            complement(11705..12511)
                     /locus_tag="PC471_000517"
     CDS             complement(11705..12511)
                     /locus_tag="PC471_000517"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HDF2787658.1"
                     /translation="MNNKPIGVFDSGLGGLTVLKEIMKILPNEDIIYFGDTARIPYGS
                     RSKETIIKYTFQAINFLKTKGVKAIVIACNTATARSLKEAQEKYDIPIIGVIEAGART
                     AVSSTKNKIVGIIGTEGTISSKAYNLEISKIDESIEIVNKACPLFVPIVEEGWANTEV
                     AKLTAKIYLQELKEKNIDSLVLGCTHYPILKRTIGEEVGEHIKLVNPAKETAKDLKKI
                     LEVQNIINNTEIHGTYQYYVSDIPEKFSDIAREFLKKKIDKIQKVEIQKY"
     gene            complement(12713..13198)
                     /locus_tag="PC471_000518"
     CDS             complement(12713..13198)
                     /locus_tag="PC471_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall hydrolase"
                     /protein_id="HDF2787659.1"
                     /translation="MATLLALTFIFMPIKEVFAYEPTENIKKVNKQEKNIAKKEVINL
                     SDNDLMLLSKLVAGEARGESYEGQVAVAAVVINRVLDPRFPDTIEEVIYQKNAFSVVL
                     DGSIKMAPTDSARKAAQEALYGTDPTNKAVYFWNPEIATCKWINRLNPYMRIGNHVFA
                     K"
     gene            complement(13249..13908)
                     /gene="spoIIR"
                     /locus_tag="PC471_000519"
     CDS             complement(13249..13908)
                     /gene="spoIIR"
                     /locus_tag="PC471_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein R"
                     /protein_id="HDF2787660.1"
                     /translation="MRKIKIRLGLLILSLISVISIMTIVINGEVKKVDNISKDYKDKL
                     IRFHVIANSNTDEDQELKLKVRDEVIKYLQPKLQNSKSIKESEAIIKKEYSSLEEISK
                     NIILKNGYNYSVKVGIQYSNFPTKQYSNIVLPAGEYKALKIIIGKGEGKNWWCVMFPP
                     LCFVDESNGVIDKSTDDKLKEVLTDKEYKLIKQDTPKKTSRVKIKFKVLEVVKDLEKK
                     F"
     gene            complement(14053..14733)
                     /locus_tag="PC471_000520"
     CDS             complement(14053..14733)
                     /locus_tag="PC471_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDF2787661.1"
                     /translation="MENLTKLNLDNYKPLRDVVFENLREAILEGKLKPGQRLMEVQLA
                     EQLGVSRTPVREAIRKLELEGLVVMLPRKGAYVANMSLKDIMDVLEVRASLEGLAAYL
                     AAERISDEDIKKLKDISEEFKKSTLESDVDALLKLDVEFHECIFKATNNKKLIQLINS
                     LWEQVYRFRVTYMSAYDTSHNIVEEHKYLLDAIIKGDSELSKKYATEHIEKAEQFMID
                     KAMISKDL"
     gene            complement(14717..15607)
                     /locus_tag="PC471_000521"
     CDS             complement(14717..15607)
                     /locus_tag="PC471_000521"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HDF2787662.1"
                     /translation="MDFIRLKSRAKINLSIDVLGKRQDGYHFVEMIMQTIDLYDIVKI
                     KELDEDEIKVKSTSLDIPLDEDNIVYKAAKILKNKFYIKKGVEIFIEKNIPVAAGMAG
                     GSSNAAAVLVGLNHLWELRLSEDELKEIGLNLGADVPFCISGRPALAQGIGEKLTNIK
                     GLPCDTNILICKPDLFVSTKEVYQGLDLNNIKKRPNNKYLIECLKSEDIKAVSESMVN
                     ILENVTIGKHKEISDIKQVMMKNNALGSMMSGSGPTVFGLFKNKEDALIGKKELLKKY
                     KQVYVVNSSQKGVEICGEFN"
     gene            complement(15725..17275)
                     /locus_tag="PC471_000522"
     CDS             complement(15725..17275)
                     /locus_tag="PC471_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3794 domain-containing protein"
                     /protein_id="HDF2787663.1"
                     /translation="MELIKDVIKVDNRIDFGKFQTFIEAEAVVPDKKLDVYEIVKTEG
                     YISLKKIEMADGKILCRGSFNYNVIYIADDKNTISNVDGKVDINEVIEKDNVIQDMEY
                     MLFSEVEHMDCTIMNERKIKVGALMNIRGSLFEKQRLDIVKDVAQVECIQKHRKEICF
                     QDIVGIEKAESSIRDTITINTEEIQSIISLNPCVKVKESRVTDNKVIIGGVLEINPLA
                     CTYEGELVELDRVGIEFTQFVEVPGVSDGMTEEVLLSMSDFNHIFKQNSESNTGLLEI
                     DCMACCKVKVTDEVTREVLQDAYSPQKIIKFDHKPIQLNKTLRRSEETFMVREGIRND
                     NDDIQIKDIVSVCPTMSIENSYIEGNKSIIQGIIKVEILFVPVEGLKLVYKISEEIPF
                     EHDIEMDNLTDTASVFNTACIDKVEVDLNRDQIDLIIKIKRFTEALDKKAENFIIKGE
                     DQGVYDLSKAPSIIVYICKEGDTFWNIAKKYNTTENEIAELNDIKLDEPIKPGKCLIL
                     EKKVVLVD"
     gene            complement(17369..17635)
                     /locus_tag="PC471_000523"
     CDS             complement(17369..17635)
                     /locus_tag="PC471_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HDF2787664.1"
                     /translation="MATVQTLDKIRVSLERHIGKKILLKANKGRKQIITKEGILEKVY
                     PSVFVIKLDDESNGYPRVSYSYSDLLTSNVKLQVFRDQDKLQIS"
     gene            complement(17783..18643)
                     /gene="yabG"
                     /locus_tag="PC471_000524"
     CDS             complement(17783..18643)
                     /gene="yabG"
                     /locus_tag="PC471_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation peptidase YabG"
                     /protein_id="HDF2787665.1"
                     /translation="MKVGDIVARKSHNKDIVFKIVSFGVDENNEKIAILKGIAFRVIA
                     DAYIDDLELVKAPDIKDILIDKNVENLLYKSVRKAKERQKRMTRAVPKLQLNPNTYGM
                     PGKVLQIDGDKEYLKICLDVYAQLGIPAVGVAVSEQNQYKEVRALLEKYNPDILVITG
                     HDAMTIKRGDIEDMSNYRNSSNFVKTVKEARKWQPNLDNLVIFAGACQSNYEKIISAG
                     ANYASSPGRIMIHALDPVFIVEKIACSRIDVVVPIDEVIEQTITGVKGIGGSETRGKF
                     RWAMPKTILH"
     gene            complement(18727..19665)
                     /locus_tag="PC471_000525"
     CDS             complement(18727..19665)
                     /locus_tag="PC471_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HDF2787666.1"
                     /translation="MKFLDLHCDTIAKLMENVETSELKSNKYSVDIDRLKKGDSLAQT
                     FALFVDTEEVKHPFDYCMSMANKFHEEMKKNSDEIALATNYEEIMKNQSEGKLTALLS
                     IEEGAVLEGKLENLKKFYDLGVRMMTISWNHVNELSFPHNKVEYREKGLTDFGREVVH
                     KMNELGMLVDVSHISDGGFYEIAKISSKPIIATHSNSRAMMNHSRNLTDDMIKVLANK
                     GGVTGINFFHLFLSDKSESKLEDMVRHIKHIVNVGGIDVVSLGSDFDGIDSKVEIEDI
                     SQMGKLYEPLKKEGFSEDDIEKIYYKNALRVIKEVL"
     gene            complement(19775..19936)
                     /locus_tag="PC471_000526"
     CDS             complement(19775..19936)
                     /locus_tag="PC471_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2787667.1"
                     /translation="MFILRFADDDDNLSVKDFTSLTDLKKYISENNIDKTWHQIEEVK
                     KVIPNLKEE"
     gene            complement(19946..20443)
                     /locus_tag="PC471_000527"
     CDS             complement(19946..20443)
                     /locus_tag="PC471_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEC-C domain-containing protein"
                     /protein_id="HDF2787668.1"
                     /translation="MSLYTEWRNLSDNHESQEAEVKFWEEYLKVEAGIYNEILNNKMD
                     VVEGKVSELAEKFGTTNEFFMGFIDGISESLKEDIVLEEVEADKEVSIKIDWEKLYNN
                     MVTVEAHWLYNLQGWEDILPADQRKEIIKAYKKSKTIVKEAKIGRNDSCPCGSGKKYK
                     KCCGK"
     gene            complement(20558..21268)
                     /locus_tag="PC471_000528"
     CDS             complement(20558..21268)
                     /locus_tag="PC471_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0489 family protein"
                     /protein_id="HDF2787669.1"
                     /translation="MENYEYSGFYIEKPVGNNVFSYDKRENKSIYVPKLINGTLNDVR
                     LGNEVVFNEVDENKEIKAKGLENMVEYVLGNKKIYVFDNHNHAFYFWAKSLLKGEFTK
                     GCKLVHVDQHKDTREPENYDVDVNNLKDVFRYTNEVLNVGSFIKPALKYNIFSELIII
                     DSLYGFDLEVESEFVLDIDLDIFSSDMDYIPFELKFYKIKNLIKKAKVITIATSPYFI
                     NQEYAIKVLKELFNYDII"
     gene            complement(21444..21974)
                     /locus_tag="PC471_000529"
     CDS             complement(21444..21974)
                     /locus_tag="PC471_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2787670.1"
                     /translation="MDIRALPIRITKEEAIEIAKSGKNSLFSKLILKNKYAHEVRLHY
                     IEFKIITLKIHKTKSIFKNRRYSNDINELKILVNGSTGSGSIVQDFPTYEVVKNVDCN
                     IVQYSDKDERRVKAKANKMAMRLTHRFMGGIPEIEIVKIESIFRPYWVVFFGKVELNR
                     RVSYLPIEADGFVIGM"
     gene            complement(21991..22221)
                     /locus_tag="PC471_000530"
     CDS             complement(21991..22221)
                     /locus_tag="PC471_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2787671.1"
                     /translation="MSKLVSKVKKDLEIKWLGIDGFLVLLYFLITGFIFYGILTRNDI
                     IVGKIIPYGVMAVVIVIFFDYIYRVLKMTRKK"
     gene            complement(22236..23630)
                     /locus_tag="PC471_000531"
     CDS             complement(22236..23630)
                     /locus_tag="PC471_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HDF2787672.1"
                     /translation="MLTFWENGIIVIFAFILLGIGYYFSRNVKDMESYYLGNRSLPWS
                     LIVGTLVASWYGGVGTVGSVEYAAIYGISAWAVWSIAAHIGRMPLALWVGPRIHIRTN
                     ITVPDLLESFYGKHVAIIGAILMFIYCAQLGEITAMGTIGNVAWKIDKELIGLIMVGV
                     VVVLTVLGGLMGVAITDMVLFFCMVFGLTMVMPGQFEQIGGFQGLTEALKDAPQLMHP
                     TKGMSAMKALMLIVYSFGAYSDPTFYQRFSAADSPKVGRRALLTCFSLWICFDMVLTM
                     TGLIVRATYPEMAPAAGYITLVLDTLPQGIRVIFIIGILGSIISTLDSYYLAAGATLA
                     NDIYGRITKKELSQKQLVKFTRIGVCLAAVMGLSVAFRFENVYDACIFVGSIWMGSAF
                     VPIVGGLLGNGRRTSMGGILGMIVGGVTFGYFKIFPVQSFELEPLVIAIPFSFIAWMI
                     GNRIGENLNTQNNL"
     gene            complement(23756..25009)
                     /locus_tag="PC471_000532"
     CDS             complement(23756..25009)
                     /locus_tag="PC471_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDF2787673.1"
                     /translation="MESKNNVKRFLIIFILAFGTTAMYSLPYMKSSFYDPMQQALALS
                     HTQIGNLLSLYGLVGMVSYFIGGWFADRFSVRKLITFSLIASGILGFYFSTFPSYSMI
                     LLIFVLWGFTTILTFFSASVKVVRMQGSESEQGRIFGFYEGLSGVSGTLISFIGLYFF
                     GKFAEITVGFKYVVWLYSAASIICGILLFFLVEEKKDSGASDEGLSIKSLLKVVTIPK
                     AWLIGLIIFSTYLVFSSLTYLSPYLSEVYVMPMTLVSALSIIRTYVIKMGASPVAGVI
                     TDKVGSSIRVMFVGFILMTVSTAAYLVIPKSTGFIWIAVINMIILSVILFGFRGIYFA
                     SVSESNISLETTGAVVGFASFIGFSPDAFYYTIAGNWLDKYGQTGYTYIFILSVVCAV
                     IGIFATYALNKINKRENKGLNNKIA"
     gene            complement(25060..26214)
                     /locus_tag="PC471_000533"
     CDS             complement(25060..26214)
                     /locus_tag="PC471_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HDF2787674.1"
                     /translation="MSNNINQDLETKIIHWGHSADPTTGALATPICQTATFAAKTVEH
                     FEELCMTWGYVYTRECNPTLTELEAKLAMLENAESAISSTSGMGAITSTILALVKSGD
                     HIVSSDGIFSHTKLFMSELLSKFGVEVTFVDAVNPQNVKEAMRPNTKIVYIESPLNPS
                     LDLVDIKTIAEIAHENKSLAIVDSTFGTPIVQRPIDLGADLVIHSLTKFINGHGDTLG
                     GAVAGSKELIDLVRWPSLCCFTGASLPPMSAWMILRGMKTLDMRMKKHCENGLAVAEF
                     LEEEENVELVKYPALKSHPQYELCKTQMNGLGGGVVSFKLKDGISGLTRDQASRRLMN
                     SLELATIATSLGEEHTLVQMNGENLIRIAVGLESSNDIINDFKQAIEKLK"
     gene            complement(26774..27343)
                     /locus_tag="PC471_000534"
     CDS             complement(26774..27343)
                     /locus_tag="PC471_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDF2787675.1"
                     /translation="MIIIYGYCRVSTKNQTDNNSLYQQQQEILNKYNDAKIIKETYTG
                     TTTNRPKFEKIISSLKKGDLLVATKLDRLARSTSEGIQLIEGLFAKEVSVHILNIGLL
                     ENTSMGKFFLTTLLAVAELERNMIIERTQLGKEFAKKKKDFKDGRPKKYSNQQIQYAL
                     GLLNSKPYREVVKMTGISKSTLIRAKKSI"
     gene            27546..27773
                     /locus_tag="PC471_000535"
     CDS             27546..27773
                     /locus_tag="PC471_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HDF2787676.1"
                     /translation="MNSLLKEAIDELKNIHCGEVFLLKELFKGYEWNRLEMNIRLKLG
                     KLFLYEVVTNPNLNVEVINKNSAYQQMYKKK"
     gene            complement(27992..28735)
                     /locus_tag="PC471_000536"
     CDS             complement(27992..28735)
                     /locus_tag="PC471_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 domain-containing protein"
                     /protein_id="HDF2787677.1"
                     /translation="MENYQSTNTCTYNILYVQFNEDFERSKYFSYDVKYPVIYNIRNQ
                     STYVNPVILNNINNAVRMTVNNFKNGLQEEEREYNNAASQNSLPKQSYWVSTSYAVTF
                     NKNYVLSMILNLMGFANNYGPKYNTLNNYNIDLTTGKELLLNDIFRDGVDYINIITDY
                     IKSQINENREMYYSNVELVIPEDQAFYLTDNGIIIYFGVDEIAPESFGIPKFELKFSD
                     FGNFINLRFYCISPDIYVQSRGRAKHFMG"
     gene            28903..29775
                     /locus_tag="PC471_000537"
     CDS             28903..29775
                     /locus_tag="PC471_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDF2787678.1"
                     /translation="MLLLADKWKDYELIDMGNGEKLERWGSYVLRRPDPQVVWPMESE
                     WALWKNPHGHYHRSNKGGGQWEHKKRYPEQWTINYKNLKFHIKPTGFKHTGLFPEQAA
                     NWDWMIEKIKKANRPIKVLNLFAYTGGATVACASAGAEVCHVDAAKGMVNWAKENIEL
                     SGLKNQTVRFIVDDVVKFVEREIRRGKKYDAIIMDPPSYGRGPKGEVWQIEEKLYSFV
                     DLCMGVLSDNPLFFLINSYTTGFSPIVLENVLSTTIGKKTNTGDIYGRELGIPASRDG
                     KILPCGIFGRWEKK"
     gene            29775..30440
                     /locus_tag="PC471_000538"
     CDS             29775..30440
                     /locus_tag="PC471_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HDF2787679.1"
                     /translation="MIKVIYEDNHLLVVEKPVNILSQGDNTNDKDMVNLLKDYIKKKY
                     NKPGNVYIGLIHRLDRPVGGVMVFAKTSKAASRLSEQVRSKTFKKTYLAVVHGKIKSN
                     SDILKDFLYKNKKTNMVTVVKKSHKDAKEAELNYNVLDFKENLSLVQVNLKTGRSHQI
                     RVQFASRKHPLYGDQRYGKEINKIGQQISLWSNKIEICHPTTKEKMEFTCHPPDKYPW
                     NIF"
     gene            30461..31318
                     /locus_tag="PC471_000539"
     CDS             30461..31318
                     /locus_tag="PC471_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDF2787680.1"
                     /translation="MSKKFSIGEMSKLHKISIQTLRYYDQIDLFKPVHVDQESNYRYY
                     SIDQFSQLDIIKYLKYLGMPLKEIKSKLDENNEEILKLLTNKINIVDEKIKELELIKK
                     VLSTKKETMEKGMKVDEVGKIVRKHIPRRSILSIEYEEGLSSEETMELSRRQIANILE
                     DNISVFYGGVSGLVSIDKFLKEDKVIYDNSFVTVERDLFDIKSQKHITEISSGDFICM
                     TYKGPYKENYTAFKKLVDYVEYKRIPIENYIYEIPIIDPLSTNNEEELLTEIQIQVKK
                     REIDLRCLV"
     gene            complement(31474..34020)
                     /locus_tag="PC471_000540"
     CDS             complement(31474..34020)
                     /locus_tag="PC471_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HDF2787681.1"
                     /translation="MNIFNKVALQGLKKNRTRTLVTIIGVVLSAAMITAVTTFGVSLM
                     NYMINGAIIKYGDWHVQFLDVDSSFVQEQRVNDEVANTATFENIGYATLEGNRNPDKP
                     YLFIAGFSKEAFDTLPITLISGRLPENSREILVSSKVATDGGVKFAVGDTISLDVGKR
                     MSENEKLGQSDPYTAGSEVFVPQDKKTYTVVGICRKPVFEESSAPGYTLITKEDKQVK
                     TDSLSLFVTLKNPHQIRSYTSSTKDHASIFNDNVLRFIGVSDNNLFNMFLYSVGGIVL
                     AIIMLGSIFLIYNSFNMSLNERTHQFGILSSVGATAKQLRNSVLFEGICIGAIGIPIG
                     VAIGIVSIGLVISVISKNFANILYVNVPLTLTLSIPAIIVAAVVSMVTILISAYIPAR
                     KAANTPVMECIRQTNEVKIESKSVKTSKLTQRVYGLEGVLAMKNFKRNKKRYRSIVLS
                     LVLSIVLFISTSALVADLKQMSNQRSEVTDQDIIFSTQGMKDNEMLKLYDKLKTANGV
                     YEGLYQKLMTYSCVAKVSELTDDYLNYEGSHSMSEMLELPVDMQFLDDRTYLNIIKSL
                     GLPAEEYTGQNAKLIAVSMVTKDNNIIDIFNSSSMNVSLTPETNGKLEIPERQNVNIS
                     FVKVVPPDSPPGIRSSDVKKASFRVLAPYSAKDKFEIPGVSTGIESLSFQSKNPTQSV
                     AEMEQIIESEGVTNEYNLYNMYGMMDENRNYIFIANVFAYTFIIMISLISIANVFNTI
                     STNIKLRRRELAMLRSVGMSEHDFQKMMNFECAFYGMRALFFGLPIAVISSWLIHKVM
                     VTDDIDFVIPWFSIGISIFSVLFIVFITMLYTINKIKKENIIDALRDDMA"
     gene            complement(34020..34706)
                     /locus_tag="PC471_000541"
     CDS             complement(34020..34706)
                     /locus_tag="PC471_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2787682.1"
                     /translation="MAFLKIEDLCKVYGKNENKVTALDHVSLTIEKGEFTAIIGSSGS
                     GKSTLLHSIAGVDVPTSGKIYLEGQDVYGQSNEKLAIFRRRQVGLIYQFHNLIPTLNV
                     VENITLPILMDKRKVNQERLNDLLELLGLKERKTHLPNQLSGGQQQRVAIGRALMNAP
                     AVMLADEPTGSLDSKNGQEIIQLLKESHSKYHQTLIIVTHDENIALQADRIICISDGK
                     VVRDERKVNR"
     gene            complement(34789..35778)
                     /locus_tag="PC471_000542"
     CDS             complement(34789..35778)
                     /locus_tag="PC471_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF2787683.1"
                     /translation="MFRNREFRKFAILFLLITILTVALGFAISIMTGILSIVSATTFG
                     IAFFVFTKNRYKSIAQISEQINLVLHNANHLYIAESDEGELSILQSEITKMTLRIREQ
                     NYALKKEKEHLADSLADIAHQLRTPLTSVTLILSLLENTSDEDERKELIRETEELLIR
                     MDWLITSLLKLSRLDAGIVVFQKEQIDVNNLISSALHQLLIPMELHNITLHIDIPKGV
                     RILGDLNWLSEAIQNIFKNCMESVGDNGKIDIICEDNFLFTQLTIHDNGAGFKKEDLP
                     CLFNRFYRGKNSSTAGYGIGLALCKTIIMRQGGTITAQNHPQGGAIFVIRFPK"
     gene            complement(35765..36451)
                     /locus_tag="PC471_000543"
     CDS             complement(35765..36451)
                     /locus_tag="PC471_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2787684.1"
                     /translation="MKRIFLVEDDKAIAKNLILLLNSEGFIVTHVSTRSEALVEIAKN
                     KFDLALIDISLPDGNGFTICTEIKEKQDIPVIFLTASGDESSVVTGLNMGADDYITKP
                     FRPRELIARIRTALRKSGRFGSTFEICGLYVDTATGVVKKNGNEVFLSALEYRLLLVF
                     ISNPKSIITRVRLLDELWDAAGEFVNDNTLTVYIKRLREKIEDDPSSPQIILTVRGTG
                     YRLGGSYVSK"
     gene            complement(36951..40157)
                     /gene="carB"
                     /locus_tag="PC471_000544"
     CDS             complement(36951..40157)
                     /gene="carB"
                     /locus_tag="PC471_000544"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HDF2787685.1"
                     /translation="MPKLDSIKKTLVLGSGPIIIGQAAEFDYSGTQACQALKEEGIEV
                     VLVNSNPATIMTDKEIADKIYIEPLTIEFIEKIIEKERPDSLLAGMGGQTGLNLAVEL
                     HDAGILDKYNVKVIGTSIESIKKGEDRDLFREVMKEINQPVIVSDIVTNLEAGLEFAN
                     KIGYPVVVRPAYTLGGTGGGIADTEEELREILSHGLQLSPVGQVLLEKSIKGWKEIEY
                     EVMRDGNGNCITVCNMENVDPVGVHTGDSIVVAPSQTLSDEEYQLLRKASIDIINAIE
                     VQGGCNVQIALNPHSLEYAIIEINPRVSRSSALASKATGYPIAKVAAKIALGYTLDEI
                     ENAVTKKTYACFEPTLDYVVVKIPKWPFDKFRHANRKLGTKMMATGEIMSIGSNFEAA
                     ILKGIRSLETGKYSLVHAPSEGRTLEELKARVVVPDDERLFDLAEMIRRGYKVEMISE
                     ITGVDKWFINKFKWIVEQEEKLKVLKIEDLDKEYLHELKKKGFSDKGISDLMKISPEK
                     LYELRSLYNIQPVYKMVDTCGGEFEALSPYYYSTYEQYDEVVVSDKRKVVVLGSGPIR
                     IGQGIEFDYCSVHCVKSLRKMGIETIIVNNNPETVSTDFDTSDKLYFEPLTEEEVLNI
                     IEKEKPEGVILQFGGQTAIKLAKFLHEKNIPILGTDFRDIDAAEDREKFDDLLERLDI
                     NRPKGKGVWTTNEGVEIAKELGYPVLVRPSYVLGGQGMEITYNEEKLIQYLDDAFDRD
                     HKNPVLIDKYLTGREIEVDAICDKEDILIPGIMEHLERAGVHSGDSTTMYPSQNISDE
                     IKEKIVEYTKKIALDLNVLGMVNIQFIEFQNELYIIEVNPRASRTVPYISKVSGVPIV
                     DLATKCMLGAKLKDLGYGTGVYKEPKLVSVKVPVFSMSKLSKVEVSLGPEMKSTGEVL
                     GVGENLEEALYKGFLAAGRHMSDERGVVLATVNNHDKDEFIEIAKDMKELGYTFVATE
                     GTAKSLRENGIEADIVNRIEEPRPNILDAIRNKQVDIVINTPTKGNDSTRDGFKMRRT
                     AIEFSTEIMTSLDTLKALVEVKKKHLNKDKLKVYNIAE"
     gene            complement(40350..41399)
                     /gene="carA"
                     /locus_tag="PC471_000545"
     CDS             complement(40350..41399)
                     /gene="carA"
                     /locus_tag="PC471_000545"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002845470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HDF2787686.1"
                     /translation="MKARLILEDGTVFIGKAFGYLEESVGEVVFNTSMIGYGEVLTDP
                     SYYGQIVTMTYPLVGNYGINLSSAESEKVQVKGFIVREKSDSPSNFRCEIDIDQYLKQ
                     NKVIGLEGIDTRALTKILRNNGTMKGIITLEDSKLEDVKHKLDKFSNTEAVRTVTRKE
                     VEHIKGNGPKVAVMDFGVKRNILRSFIARGCDITIFPATTSPEDVLSINPDLIFLSNG
                     PGDPEDLEDVIENIKALIGKKPIVGICLGHQLLALALGGKTAKLKFGHRGGNHPVKDL
                     EEGKVFITSQNHGYYVSEVPEQMKVTHINLNDNTVEGMRHEKLDVYSVQYHPEACPGP
                     KDNDYIFDKFLELVK"
     gene            complement(41656..44862)
                     /gene="carB"
                     /locus_tag="PC471_000546"
     CDS             complement(41656..44862)
                     /gene="carB"
                     /locus_tag="PC471_000546"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HDF2787687.1"
                     /translation="MPKLDSIKKTLVLGSGPIIIGQAAEFDYSGTQACQSLKEEGIEV
                     VLINSNPATIMTDKEVADKIYIEPLTIEFIEKIIEKERPDSLLAGMGGQTGLNLAVEL
                     YEKGILDKYGVKIIGTSVESIKKGEDRDIFREVMKEINQPVIVSDIVTDLQAGLDYAL
                     TIGYPVVVRPAYTLGGTGGGIADNEEELREILSHGLQLSPVGQVLIEKSIKGWKEIEY
                     EVIRDSKGNCIVVCNMENIDPVGVHTGDSIVVAPTQTLSNKECAMLKKASLDILNAVE
                     VQGGCNVQFALNPHSFEYAVIEINPRVSRSSALASKATGYPIAKVSSKIALGYTLDEI
                     ENAVTKKTYACFEPTIDYVVAKIPKWPFDKFKKANRKLGTKMMATGEIMSIGSNFEAA
                     ILKGIRSLETGKYSLVHTPSEERSIEELKKRVVVPDDERLFDLAEMIRRGYKVEMIEQ
                     ITGIDKWFINKFKWIVEQEEKLKVMKIEDLTKDYLLELKKKGFSDKGISDLMKISPEK
                     LYELRSLYNIKPAYKMVDTCAGEIDAISPYYYSTYEQYDEVVVSDKKKVIVLGSGPIR
                     IGQGIEFDYCSVHCVKSLRKMDIETIIINNNPETVSTDFDTSDKLYFEPLTEEEVLSI
                     IEKEKPEGVILQFGGQTAIKLAKFLHEKNIPILGTDFDDIDAAEDREKFDDLLERLDI
                     NRPKGKGVWSLNEGIEVANELGYPVLVRPSYVLGGQGMEITYNEEKLSQYLQDAFDRD
                     HKNPVLIDKYLTGREIEVDAICDKEDILIPGIMEHLERAGVHSGDSTTMYPSQNISDE
                     IKEKIVEYTKKIALDLNVLGMVNIQFIEFQNELYIIEVNPRASRTVPYISKVSGVPIV
                     DLATKCMLGAKLKDLGYGTGVYKEPKLVSVKVPVFSMSKLSKVEVSLGPEMKSTGEVL
                     GVGENLEEALYKGFLAAGRHMSDERGVVLATVNNHDKDEFIEIAKDMKELGYTFVATE
                     GTANSLRENGIEADIVNRVEESRPNILDAIRNKQVDIVINTPTKGNDSTRDGFKIRRT
                     AIEFSTEIMTSLDTLKALVEVKKKHLNKDELKVYNIAE"
     gene            complement(44915..45958)
                     /gene="carA"
                     /locus_tag="PC471_000547"
     CDS             complement(44915..45958)
                     /gene="carA"
                     /locus_tag="PC471_000547"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HDF2787688.1"
                     /translation="MKGKLILEDGTVFEGKAFGYLKEAIGEVVFNTSMAGYGELLTDA
                     TYYGQIVTMTYPLIGNYGINLEDVESNKVQVKGLIVREKSDNPNNFRCEMDIDTYLKQ
                     NKVIGLEGIDTRALTKIIRNNGTKRGIITLENVSLQDVQSKLEEHCNKDSVKNVTRKE
                     IENIKGTGAKVVVIDFGVKNSVLESLKACNCDITIVPAMTTVEEILNINPDLILLSNG
                     PGNPEDINDIVENIKKLIGKKPLTGIGLGHQILALALGGKTSKLVFGHRGGNHPVKCL
                     ETGKVFITSQNHGYNVSEMPENTEVTHINLNDNTIEGMRHKELPIYSVQYIPEYTTKE
                     DLDCIFNKFLALI"
     gene            complement(45984..46700)
                     /gene="pyrF"
                     /locus_tag="PC471_000548"
     CDS             complement(45984..46700)
                     /gene="pyrF"
                     /locus_tag="PC471_000548"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HDF2787689.1"
                     /translation="MINGKEKLIVAIDTDEFDKAKELIDRLEDSVDIFKVGLEQYVAT
                     KGKTIDYLKEKGKKIFLDLKFHDIPNTMKSAVKAAVRDNVWLMTIHVSDMEGMRQCAL
                     IAREEAERLNIEKPLVVGVTVLTSLSNQDLQDIGCNMTTEELAIKRAKLAKESGIDGV
                     VCSAQEVDKIVEACGTDFITVCPGIRPKSAEVGDQKRVVTPSDAIKKGAHYLVVGRPI
                     TKAENPRESAINIVREIENA"
     gene            complement(47076..47711)
                     /locus_tag="PC471_000549"
     CDS             complement(47076..47711)
                     /locus_tag="PC471_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1847 domain-containing protein"
                     /protein_id="HDF2787690.1"
                     /translation="MYKCGKCNLRGCSNGNMDKLMKICPTREIELQQKAKQLYEEEEN
                     RKIAYNAGLVEAEGYCQYTRLKEIIEFAKKCGYKKIGIAFCIGLKREMQTVQKIFEHH
                     GFEVFSVICKNGAMPKSTIGLEASQTISKCSDEVMCNPIGQALFLNEAKVDLNVILGL
                     CVGHDTLAMKYMEAPITTLVVKDRVTGHNPVAAIYNAESYFEKKLFDKDEE"
     gene            complement(47735..48649)
                     /locus_tag="PC471_000550"
     CDS             complement(47735..48649)
                     /locus_tag="PC471_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2787691.1"
                     /translation="MELYEAIFYRKSVRNFSSKKIKKPLMEEIKRSCKNLEYLNEDLN
                     IKAHVVDRGHIIHFLMGKECKVKAPHYIVVTSDKGEDYLQNIGFATEGVVLRLTTLGI
                     ATCWLEGNLKREDILEFVDIGNKNFEEDEEDDLDYLAGIKTEEDDENKIENPYIIIAF
                     GYPAKNEELFRTRYARPDRKPVKDMINKINRNRKWKDILELTRRAPSVKNSQPWYFHK
                     DERGLHLFEKRPKKHCEDMNKVSLGVALRHFDIACIKNKIDVSYEKLPIRNKIGKSYF
                     ITVVEHVKPEEETQEENVTLEKEESQDE"
     gene            49250..50479
                     /locus_tag="PC471_000551"
     CDS             49250..50479
                     /locus_tag="PC471_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HDF2787692.1"
                     /translation="MNLNRNLEKYAELAIKVGVNIQPGQILLIKSPIECADFARNALK
                     EAYKCGAKNVYIEWSDEESTLIKYLYAPDEAFHEFPKWTAEQYVDIAKEGGAFLSVYA
                     QNPDLLKDVDPERVANFQKASGKALKEWRSYTLTDKCKWSIVSVPTKDWAKKVFPNLS
                     EDKAIEKLWDAIFKCSRVDGQDPIKAWEEHNENLKSKMDFLNKNNFKTLKYKSSKTDL
                     TLDLPKGHVWLSGASKDPNGISFNPNIPTEEIFGMPHKFKVNGTVYSTKPLVYGGNII
                     DNFFLTFKDGKIIDFSAEKGLDTLEKLIETDEGSHYLGEVALVPYNSPISNTDIIFYN
                     TLYDENASCHFAIGSAYKTCLEGGNNLKDEELDEHGVNDSLTHVDFMIGSADMDIIGE
                     TYDGKQIQIFKNGNWAF"
     gene            complement(50684..53065)
                     /locus_tag="PC471_000552"
     CDS             complement(50684..53065)
                     /locus_tag="PC471_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HDF2787693.1"
                     /translation="MEIRQYLKNNILIFDGAMGTMLQQKGLKLGENPEVFGLQNPDKL
                     IEIHTAYLEAGSNVILTNTFGCNELKLDSKYTVEEVIDNAVLVARKAIENVDNTKPRY
                     VALDIGPIGEMLEPMGTLSFDKAYEIFKRQVLQGVKSGVDVIVIETMMDLYEAKVAVL
                     AAKENSDLPIFCTMTFDEGGRSFTGCMPECMVATIEGLGVDAIGVNCSLGPKQLLPIV
                     EKIASRATVPVMVQANAGLPNIVDGEAIYDVDAKEFFEGVKKFVEVGATIIGGCCGTN
                     PSFIKEISENINSVTKGCIEKIDKCVVCSPSKFVEVQSPTVVGERLNPTGRKSLQEAL
                     KNENVDYAINLGLEQVNAGAQILGVNVGLPEIDEKKLMPKLIREIQAVVDTPLQVDSS
                     NVEALEQGLRYYNGRTIVNSVNGKEESLESILPIVKKYGSCVVGLTLDEKGIPKKAEE
                     RFEIAKRIVNRAVSYGIKPKDIFIDCLSLTVSAQQEEAIETIKAITMVKTLGVKTILG
                     VSNISFGLPNRKALNASFLTLALGAGLDLAIINPNEYSMMEAINSFKILNNTDKGCIN
                     YINQYSNINNSKKSDSSTKIDKDLPLDILVERGLKDEAKNVTLNLLKEKDENYILDEI
                     LIPALDKVGKRYDSGDIFLPQLIQSAETVKVSLNTIKETLLSKSSNNVSKGKIIVATV
                     KGDIHDIGKNIVKIMLENYGYEVIDLGKDVPIEEVVEVAKKRNIKLVGLSALMTTTVQ
                     SMKDTIQALRDNEINAKVFVGGAVLTEEYAEEMGADYYSKDAKSAVEIAKLNF"
     gene            complement(53037..53762)
                     /locus_tag="PC471_000553"
     CDS             complement(53037..53762)
                     /locus_tag="PC471_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Vitamin B12 dependent methionine synthase,
                     activation domain protein"
                     /protein_id="HDF2787694.1"
                     /translation="MENNLIGREDISINQSEVLRYLQYRGQEIDKDLKYTIDECVAIT
                     KNKINPRYLSRIYPIKINKDNVVELEGTNLTLESKDVYELLKECNECVIMAATIGIDI
                     EKEIRRHSYSNLTKGLIIDSCGTTAIEEVCDIVQNNLEKVFLKQDKFITMRYSPGYGD
                     LPIEKNIDILNVLNAQKQIGLTITSNGIMIPRKSVVAIIGISNTRINKSKKEKSCENC
                     KNYRNCTYRKGANSCGNKTISEK"
     gene            complement(53752..54207)
                     /locus_tag="PC471_000554"
     CDS             complement(53752..54207)
                     /locus_tag="PC471_000554"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HDF2787695.1"
                     /translation="MEKVESFKLDHTKVKAPFVRKCSVLDGVKGDKVTKFDLRFLQPN
                     VESFGTAAMHGLEHLLATYLRDTLDGVIDLSPMGCRTGFYLILWGDVDAKTVKIGLEE
                     ALKKVLESDKMPAATAIECGNYRDLSLFGAKEYAKDVLDKGFSLNIYGE"
     gene            complement(54489..55670)
                     /locus_tag="PC471_000555"
     CDS             complement(54489..55670)
                     /locus_tag="PC471_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HDF2787696.1"
                     /translation="MKNKSLLNHMFRPILDLIISCALTSMLILTSMYIIKSLYVNSDM
                     SVVNQLVYQARDFRSLIGYDLFYPFLFTITSLAIYSMISYKRNKQIVDFIRKTNRKNQ
                     KLEIIKSAVNLMDEGNLEKSIDIEYDLDILKDKEKDDIDELAHKINNIVHQLRNITIE
                     ERQAQQTKTDLITNVSHDLRTPLTSIMGYLGVIEEGKYKDEVQMMYYVDIAYEKSKNL
                     NVLINDLFELTKMQNNTIKLNKIEINLVELLSQVVSQFEIYFKQEFMVSRINFNEEKL
                     IVKADPNKLVRAFENLITNAVKYGKDGHYVDIVTEKKEDMAVVKVINYGEPIPVLDLP
                     NIFDRFYRVEKSRNRNVGGSGLGLAITKNIVNLHDGEITVSSDKYQTVFEVQLPIIQS
                     A"
     gene            complement(55660..56352)
                     /locus_tag="PC471_000556"
     CDS             complement(55660..56352)
                     /locus_tag="PC471_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011948339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2787697.1"
                     /translation="MNNTVLVVDDEKEIRDLIEIYLLNSGYNVIKAEDGKEALDILKK
                     ENIHLMILDIMMPKMDGIEVCRKVRESLNIPILMLSAKSEDMDKIQGIMTGADDYLTK
                     PFNPLELTVRVKSLIRRAYYFSGANNEDKDVIKVGVVIIDKEKHSVTVAGNEIILTSR
                     EFDILYLLANNKGRVYSTEEIFEKVWKEKYYQSNNTVMVHMSRIRDKIEKYTDGEKII
                     HTVWGVGYKIEK"
     gene            complement(56486..57202)
                     /locus_tag="PC471_000557"
     CDS             complement(56486..57202)
                     /locus_tag="PC471_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HDF2787698.1"
                     /translation="MKKFKLIGAIILVVFISVFISKDRLLKKDKDPIVAAQHDSEQDV
                     EANSQNKNHSNSISNILLVNKTNGISKNYTPENITKVNIPFVEEATEEEKQMAGEPAK
                     AVEDLVKQANSEGIQFLGSSAYRSYDTQLDTYTRRVKSQGREKADAYVAKPGYSEHQT
                     GLCIDLTNPERWFVGSTKEAKWLAENAHKFGFIIRYPEGKEDITGTAYEPWHIRYVGK
                     DAAEEIYSKGLTLEEYLQNK"
     gene            57327..58061
                     /locus_tag="PC471_000558"
     CDS             57327..58061
                     /locus_tag="PC471_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HDF2787699.1"
                     /translation="MSIQKLLSKARKAIQDFDMIQENDKIAVGLSGGKDSLTLLHILK
                     SYQRFSPQNFELIAITLNTGGVDNSPLDKLCKEINVPFYEFQTDIKEIVFDIRQEKNP
                     CALCANLRRGALNDNAKKLGCNKVALGHHKDDAIETFLMSMFYEGRVNCFSPKTYLDR
                     QDLTVIRPMVYIEEYMTKKISKDSNYPIITNPCPANGHTRREYIKNLIANLNKEMPDF
                     KRNVFGALNNSEKLFIWDKEKIKNFK"
BASE COUNT        18228 a        10782 c         6443 g        22739 t
ORIGIN      
        1 tttcgtcaat actttttttg ttttttattt taaaaaaggt tataactttt ggttttttta
       61 ataaatataa ttaaaactaa tagatataac ccttgatatt acaaacaata catgcatata
      121 taattttacc tattttgcaa ttttattttc ataagaatat gaattagctc tcactctatc
      181 tgcccaaaca tttatactct taactttatt aacattagtt gtaccctcaa aaatatccat
      241 tgcatcctta caaaaatata tcataacatc aagaaccaaa aatccaacac aaaataaaca
      301 taaaaaatta attacttgaa acatcagaca ttcccccaat aattttttat atatagtatt
      361 ctcataaatt attcttttta ccttctcata aattattcta aatattttca taataaatta
      421 ctttcaatca tattttctaa atataaagtc tcaatcaaca aaaactaata tcaatttcta
      481 acgtttgttg gtaatttata aattaaatat ctaaaagaat ctttgaagca tataaaaagc
      541 attccacact gacattttaa ataaaattta gctatttatt gataatttaa tagctaaact
      601 ttatttaaaa cttagtagaa tgctaatatt tcttagtttt cttcttcttt atttggattg
      661 ttatcaatag gtttcatagt cgggaataat aatacatctc ttatagatgg agagttagtt
      721 aataacataa ttactctatc tatacctatt cctagtcctc ctgtaggagg tagacctacc
      781 tctaatgctt ttaagaaatc ttcatccatt tcaaaagctt catcatcacc taattctctt
      841 tttctcaatt ggtctataaa tcttcctttt tggtcaatag ggtcattaag ttcagaaaat
      901 gcattagcta actcccactt attagcaaat gcctcaaatc tatcagttct tcttgggtct
      961 tcaacatttc tcttagataa tggagatact tcaactggat ggtgagttat aaatgttggt
     1021 tggattaatt tatcttctcc aaattcttca aagaaagcat ttattacttc tcctcttctc
     1081 attccaggtt ttatctctat acctttttct ctagcaactt ctagtgcctc ttcatctgta
     1141 tttatagtag aaaaatctac accagaatat tctttaacac aatcttccat actcattctt
     1201 ttccaaggtg gtgtaaagtt tatttctgtt ccttgatagt taactatcat acttcctgta
     1261 gcaacttcag ccatatgtgc aataacattt tctgttattt ccatcatatc agtatagtct
     1321 gcatatgctt gatataattc tattgcagta tattcaggat tatgttttaa atccatacct
     1381 tcatttctaa acattcttcc catttcataa actttatcaa atccaccaac aataagtctt
     1441 tttaaataca attcattcgc tattcttaaa tacattggta tatgtaaagt attatgatta
     1501 gttataaatg gtctagcatt tgccccacct gctatagtat ttaatattgg agtctcaact
     1561 tctaaaaatc ctctatcatc taagtatgct cttaatgctt ttaatgcttt tgttctaatt
     1621 aaaaaagcat tttttacttc tggatttact attaagtcaa catatctttg tctatatctt
     1681 agttcttggt cttttaatcc atgatatttt tctggtaaaa cttgtaaaga tttacatagt
     1741 aatactacag atttagcctt tacagatatt tcaccttttt tagttttaaa gatttctcct
     1801 tcaattccaa ctatatctcc aatatcatat gttgaaaata cactatattc ttcctctcct
     1861 atagcatcta gtcttacata agattggatt cttccctctt ggtcttgaat atctataaag
     1921 ccagctttcc cttgtattct cttgctcatt atacgtcctg ctatttttgt agttttaccc
     1981 tcaagagaat caaagttatc ttttatatcc atagaataat gagttctatc atatctactt
     2041 tctttaaaag gatctctacc tgactcttgt aattttttta gtttatctct tcttacttga
     2101 agtacttcac taaggtcttc ttgaatttgc gcttcttcat tgctttgttg attatttttc
     2161 atttttttcc tccaaattat cttcttattt ctaatatttc aaacttagca actccatcag
     2221 gaacttgcac ttccacaact tctttcgcag ctctacctaa aagtgctttc cctacaggag
     2281 attcgtttga tattttacca tcatatgggt ctgcttctgt cgaaccaact atagtatatt
     2341 caacctcttc atcaaattct aaatccttaa ctttaactat tgatcctatt gtaacaacat
     2401 taaggtctat tttgctttca tctatgataa cagcttttct aaccatattt tctaatttta
     2461 aaattctttc ttcaacttgt gcttgttcct ttttagcttc atcatactca gcattttcag
     2521 ataaatcacc aaaagatata gcaactttta ttctctctgc aacttctttt ctctttacaa
     2581 cctttaagtt ttctaattct tcttctaatt tatcatatcc ctcttgtgtc aataaaaact
     2641 ctttattttc ttccattata aatacaactc cttcacaaat ttcttaggaa ttttcctaat
     2701 tatattttaa aaattttata atcttttttt aatttatgcg ctatattata agtcactatt
     2761 atttttatgt caaggagtct tccatatagt actccaattt cgacacaact tcttgatagt
     2821 cttctatttt gttaatttca tcccttaatt ttgcagagtt tctaagtcct tttaaatacc
     2881 aagcgatgtg ttttctcatt tcccttacag ctacatactc tccatgttct tctactgcaa
     2941 gttttaaatg cttaatggca gtacttattt tttcgttaag agttggctct ggcaaaatct
     3001 cgcctgtatt catataatgg tttattcttt taaatatcca agggttacct tgagcacccc
     3061 tacctatcat tatagcatca caaccagttt tgtctaacat atttattgaa tcttctattg
     3121 taaatacatc accatttcct ataacaggta tactcaaatt tttcttaatc tctgcaatta
     3181 tatcccagtc tgctttgcct gtataaaact gttctctagt tcttccatgt attgctaaag
     3241 cacttattcc acaatcctct gctatctttg ctatttcaac agcatttaca ctattatcat
     3301 cccagccttt tcgaatcttt aaagtcactg gcttttttga gttcttcact actgccctca
     3361 atacctcttc tgcaagctta ggatttttca taagtgctga accatctcca tttttaacta
     3421 cttttggagc tggacacccc atatttatat cgagaatctc atttgaataa tcattcatta
     3481 tctctgttgc tcttcccata aactctggtt ctgaaccgaa tatctgaact gctacaggat
     3541 gttcttcctc ttctatctta agcatttttt tagtattttc atcatcatag caaagtgcct
     3601 taccatttat catttctgta tataaaagcc cacatccttg ttctttacaa attaatctaa
     3661 atggaaggtc tgttacacct gccataggtg atagaaaaac tttatttttc aattctaaat
     3721 ttcctatttt cattgttttt ctcctatttt tgcctttatt ttgtgattta actttaaatt
     3781 cttatttcct cattataata taccacatat agctagaaat ctatcataaa aagggaattg
     3841 gatatataac ccaattccct gtatattaat tattcttggt ttttttcata tattattctc
     3901 aatccttcta gtgttaaaaa accatctaca taatctatac ttttcgtctc tgaagcaatc
     3961 agtttagcta atccacctgt agctataacc ttaacatcat cacaattcaa ttctttcttc
     4021 atcatactta ttattttgtc aactaaacca acatatccat aaattattcc agattgcatg
     4081 gctgatacag tactcttaca aatagtcata cctggcttag ctaattctac tctaggtaat
     4141 ttagacgcac tttgaaataa cgcctcacta gatattttta ttcctggtgc tattgttcca
     4201 cccaaatatt cacctttttc agagatagca caaaatgtag ttgctgttcc aaaatcaaca
     4261 agtatacttg gtgctccata cttttctatc cctgctacag cattaactat tctatctgcc
     4321 ccaacttgtt ttggattatc atattttata tttagacctg tttttatgcc tggacctact
     4381 attaatggct gttttttaca gtactttata caaaagtttt caagagaatg cataacattc
     4441 ggaactacag atgatattat aacatcatca atatcactta tattcacatt atcataatca
     4501 aataggttac ttatcaggat tccatattca tcagacgttt tttccctatc tgttttaatt
     4561 ctccagtaat taactaattt gtcaccttta tatataccta aaaccatatt agtatttcca
     4621 acatcaaata ctagaagcat atgtcattaa ccttctttct attttttgct tttcttaagt
     4681 acagtggcaa cagcagctac tactaaagcc cctactattg cttctggcac cccatttgct
     4741 gcggcaagag ccattattac ttttcctgct gaaccacttt tacccattgc atgaagatac
     4801 ttgtcagcat ataagacata tatcattcct aaaactccag ctgtatttaa cagagaacct
     4861 attgcccctg taattaaacc tgatatgtat accttatttg tagccttaat tattaactga
     4921 tatacataat aagctaatac acctatcata actcttggta atattgaaac taatggattt
     4981 ataaaaacaa aagatgttgc tgttgggttt gttatagcat ttaagagact tgatatccca
     5041 aatataaatc ctattgctgc cccaactatt ggtccttcca taatagctcc aataataact
     5101 ggtatgtgca ttatagttgc ttttgtaggt ccaactggaa taaatcccaa tggagtcata
     5161 gatagcataa tagaaatagc tgatagtact cctattatag tcatctttct tacatttact
     5221 tttcttactg atgcacttgt gttcatcata aaaaatacac ctccgttcca gctcattaag
     5281 atgccggact acttaatttt ttaatttttt tcgttttttc ttataactat gagtcaagct
     5341 accattgtag gatgcccgct ctaacaagaa taattttatc aagatgagtt gccattgtca
     5401 acaatttgac aagaaaaata tgctaatttc atcatatttc tcgttttttt actatatctt
     5461 tatatgccat tatattttct acactatccg ctttttttac tgcatttatt atactttttt
     5521 ccacaacctc tacagccaat gaacctacta atgttatatc tgcttctatt tctcctgttg
     5581 atattgtgaa tattgtatct ccatcatgag gtgtatggat aggaaaaata gacttagcat
     5641 atccatcatg tcccatttga gatatctttt tacatccagc tttggttaac ttagcatttg
     5701 ttgcaattat cccaattgta gtattatcta cactaaatcc acctttactt tcaccttgtt
     5761 tcattaattt atatgtatca attgtttctg tctttttatc atttaaagca ccagctataa
     5821 ccttaccatt ttcataaaca tctccaagag catttacagc aattaatgca gatacaatta
     5881 gaccattatc taattttata gaatagctac ctattccgcc tttcatagca tattcctgac
     5941 ctcttatctt tccaacagtg gctccacaac ctgctccata atttccttga tttaaaatat
     6001 cagtatttgc aacttcacaa gctctataac ccatctttaa atctggtcta catttaaagc
     6061 tccctactcc taaatcaaat aaaactgctc ctacaactat aggcacttta cctacaccta
     6121 catcaaagcc aattcctttt tcttctaagt atctacttac accacaagtg gattcaagac
     6181 catatgcaga acctccagcc aaaactactg catgaacttt ttgaaccata tttacagggt
     6241 ctaataaatc ggtttctcta gtacctggag cagagcccct tacatcaaca ccgcaagtag
     6301 ctccttcttc acatattact actgtgcacc cagtaagacc atttctatcc tcaacctgcc
     6361 caacctttaa gccttctata tccaaaatat tattatacat atcctttttc tcctcttata
     6421 gaaacttcac ctgaaataat ttcttttata cttccatcac ttctttgaac taataaattt
     6481 ccctcttcat ttatgtctaa acatttcact agttctctat tatctccttt taatacatat
     6541 atatttttat ctataattgc agaataatct ctacatattt ttaaggtttt ttctttggaa
     6601 ttatttacag tataatctaa atataatttt tcaaattcag ttaatacatt cctaacaata
     6661 tctactctgg ataacatata tccttcttta tacaatgatg tggctttgtc agctatttct
     6721 tgttcaaaat ccattatttt aacatttatt ccaattccaa gaacaacata atttgttctt
     6781 tcaatttcag cagataactc tgttaatata ccacatattt tttttccatt taaaataaca
     6841 tcatttggcc attttattaa tgtatttgct cctagattgt ttaaagcttt cgttacactt
     6901 gctcctgcta ttaaagttat aaatggggct ttgaatggtt ccatatctgg ttttaatata
     6961 atactcatcc agattccttc atgaggtttt gaatcccatc ttcttcccaa tcgtcctttc
     7021 cccatatctt gatgttcact taaaatcaca gtcccatcta cactatttat agctatcctt
     7081 ttagcatgtt catttgttga atctatagtg tcaagatgtt caatattttt tccaataaac
     7141 tctgtatcta atttatagta aatattctgt gggcttaata aatcagttgg attttccgct
     7201 aatcgatacc cttttttatt tacagattct atattatatc cctcttcttt taaggctttc
     7261 atatgtttcc atatagctgc acgtgacacc cctaattgtt ttgataattc ttctcctgaa
     7321 ataaattcag atttattatt taatataatc tctattattt tatctctcat atttcacctc
     7381 taatatgaca taatttcatg acttattttc ttctaataaa taattataca ttattaatat
     7441 acttttaata ctattaattt taaatttcct ttttaattat tataatggtt gttttatatt
     7501 ttatattgat gatttatata attttacatt ttatttaaat aattttaaaa taataaaaaa
     7561 actgaaagta tcaattaaat taatacatgc aaatataatt tgcttattaa tttaatatta
     7621 tactttcagt tttagtattt aatccacatc agataataat cttaaaatga ccaaggtctt
     7681 aaaactattt atctttatca gatttatctt ctaaattttt atttatatca ataacattgt
     7741 ttctttcttc ttcatctttt tctaatttta tagttaattg ttcttcaact tctttagaag
     7801 aattttcctc tgtaactgca ttttcaattt cattcagagg taactcttta ttaaatgctt
     7861 ttacaaattg ttctgcatct aaagtttcat atataagtaa agcttgtgct acatactcta
     7921 atctatccat attttcttgt aatatacttc ttgtcttatc atatgcacca tcaactatac
     7981 gctttgtttc ttggtctatc tcaaaagcaa cttcttcaga ataatttctc ttacttgaaa
     8041 aactattacc taggaatact tcatcatcac tgtcaaatga cattggacca agtttagaac
     8101 tcattccata ctttgttacc atacttcttg cagtagctgt aactctttct aagtcatttg
     8161 aagcacctgt agatacatcc ttaagtacta gctcttctgc tactctacca ccaagtaaaa
     8221 ctataatatt ttctttcatt tcattttttg tagcatagaa cttatcttca acaggaagtt
     8281 gcattgtaaa ccctccagct cttcctcttg gaactattgt aacttgatga acaggactta
     8341 cttcttcaag tacatgggca caaactgcat gccctccttc atggtatgct gtaagtcttc
     8401 tttctttctc acttattact ttcgattttt tagccacacc tgctataacc tttgttatag
     8461 cttcttctat agtttccatc ttaattttct tttctctttt tctagctgtt aatattgcag
     8521 cctcattcat taagttctct atatcagcag gagtaaatcc tggtgttctt ctagccaaga
     8581 catccatttt tacatcatca cttaatggtt tatttcttga gtgaacttta aatatagcct
     8641 ctctaccttt tacatctggt gtaccaacaa caacttgtct gtcaaatctt cctggtctaa
     8701 gtaaagctgg gtcaagtata tctggtctat ttgtagctgc catgattatt ataccttgat
     8761 ttaccccaaa tccatccatt tcaactagaa gttgattaag agtttgctct ctttcatcat
     8821 gacctccacc aagacctgca cctctttttc taccaactgc atcaatttca tctatgaata
     8881 tgatagctgg agcacttttc ttagcctgtt caaataaatc tcttactctt gaagcaccaa
     8941 ccccaacaaa catctcaacg aaatcagaac cacttatact gaaaaaggga actcctgctt
     9001 ctcctgcaac tgctcttgat agatatgttt taccagttcc aggaggtcct accataagca
     9061 ttccttttgg tattcttgca cccaattcaa tatatttctt aggattcttt aagaaatcta
     9121 ctacctcctg caaatcttct ttttcctcat ctagtccagc tacatcttta aatgtaactc
     9181 tagttttttc atcatcttta tgaaccttag cttttgattt gccaaaactc ataacctttc
     9241 cgcctccacc ttgagactga ttcataaaca cgaaccaaat aatcaccatg aagaatatta
     9301 acagtaatgt aggcaacatt tcgacaaacc aaggtgtaga tggcttagct tcaccaccaa
     9361 aagttagttt cccagcctta gcttggtcta acacctcatc tgcaagttta tttcccatca
     9421 cttctgttgg aacatatgac ttaaactttg tatttgtatc ttttatagta ccttctacag
     9481 aagtttgatt tacaaagtat aatcttgata tattctcatc tgttaattct ctatatactt
     9541 cagagaattt taaatctttt attttttctg taggcttacc tgagaattga actataccta
     9601 ctataattat aaaaacaagt agataaaaac ctgccccttt tagcaactta ttcaatatag
     9661 aagccctcct ttcatacatt gctttgccct ataatttgtt aaaattaatt tttaatactt
     9721 cttttgtgct ttcatcaatt ttataatttt cactgatttt ataatctcca acacatatta
     9781 ttccttcacc atctgtgatt ataggaattt tacatctttc ttctcttggg atttttaaat
     9841 ctatgaataa atctttcact tttttacttc ccatagcaag ctttatctta tcaccttgcc
     9901 ttctgcttct tattactata ccccctttta ccttattaaa atcaaaccct ttagatgaat
     9961 tgtctaattt catactctta tacctatcta tgctcattac ttgggtctca ataatttgtt
    10021 ttaattcttt tattttaata aacccattac taggtacatt gtaataaaat tcaatttcct
    10081 cattaacaat ttcctcattc gttaatatta tactgtcttt ttttctataa acaaatatat
    10141 ttcttggaag agttaacatt ttatttactt ttgaattatc ttctaaagac attatatctt
    10201 ctatatgtct ttggtcaaca aaattagtat ctcctagtat atgttttatg gaatttctaa
    10261 gaactctaac tttgatagca tcatgtaagc aaacaaaatc gtctaaattt atctctacaa
    10321 agcctttttc ttttatattt gaaacttctc taaatttagc ttctgcctct ttctcaatat
    10381 aatcgttatc actttttaaa ctattgctca tcctcactat agattctatt acatttgaat
    10441 taaaattatc cttcatatat ggtaaaaggt ctaaccttat cttatttcta gtgtatatat
    10501 tttctaaatt tgttttatct atcctcggat ttaaattata agcttcacaa tactcctcta
    10561 tctcatttct ttccacgtct aatatgggtc taattatgca attatctcta atataatcaa
    10621 ttccctttaa gccttttaaa cctgttcctc tcatgatacg catcataacc gtttcggctt
    10681 ggtcattgag attatgacct attgctattt tattggcttt aatcttatcc ttaatttcat
    10741 aaaacatttc atatcttagc ttccttgctc cttcttctaa tgataatccc tcattttcac
    10801 aatattttgg tacattaata gactttacaa agaatataat tcccatatcc tcacaaagtt
    10861 tagatacata taatgcatct ttttgagctt ctatccctct tatttgatgg tttaaatgtg
    10921 ctgcatatat ttctatatta aaatcttttt ttaatctatt tagtacatgc aataaacaaa
    10981 ctgaatcagg tcctccagaa agacctaaaa ctattttatc gcctttttgt attaaattat
    11041 gtttatttat agtacttagt accttatcaa atatcatatt tatccctctt acacttagtt
    11101 ttattcactt ttaagtatac tttctataaa aaaacgctaa acccttgtat caattttaag
    11161 aaattaaagt ttaatataaa tataataata ccaaataaat taaataaaag gtagtttttt
    11221 cactaccttt ataaaatttc tggaaaatag ccatagttat tatttagtac ctatgtatat
    11281 atctatttta ttcctaccta aaaataaatc ttgtaccttt atattatagt ctattttcaa
    11341 ttttatgtgt tccccttctt gtatatttat atctaattct cttgtatcaa tatcaattaa
    11401 taaagtacct tgtggggtca catactttgt agtactactt tctccacatt taaacagcat
    11461 tgttgagtta atagcaccaa actttttgat actaacttga tttttaagta tctttatagt
    11521 attagttatt tctatatctt cttctttttc cttgtaaata acatatattc catcattttt
    11581 ataaaaaatt ttaccaaagg cagtcatctc tatggtgtcc atatttcctt tttcatcata
    11641 ttgtctagta tttatactta tttttacact caacgatttt tcctccatag attcttcata
    11701 taaactagta tttttgtatt tctacttttt gaatcttatc aatttttttc tttaagaact
    11761 ctcttgctat atctgaaaat ttttcaggta tatctgatac ataatattga taagttccat
    11821 gaatttctgt attgtttata atattttgca cctctaaaat cttttttaaa tcctttgcag
    11881 tctctttagc tggattaaca agttttatat gctctccaac ttcttcacct attgttctct
    11941 ttaaaatagg ataatgagta catcctaaca ctaaagaatc tatatttttt tctttcaatt
    12001 cttgtaaata tatctttgct gtcagtttag ccacttctgt atttgcccat ccttcttcaa
    12061 caataggcac aaataaagga catgctttat taacaatttc tatactctca tctattttag
    12121 atatttccaa gttgtatgct tttgaactta tagttccttc agttcctatt attcctacaa
    12181 ttttgttttt tgtagaactt acagctgttc tagctcctgc ttctataact ccaattatcg
    12241 gtatatcata tttttcttga gcttctttta agcttcttgc agtagcagtg ttacatgcta
    12301 taactatagc ttttacacct tttgttttta aaaagtttat agcttgaaaa gtatatttaa
    12361 ttatagtttc tttggatctt gaaccatatg gtattctggc agtatctcca aagtatatta
    12421 tatcctcatt tggtaatatc ttcattatct cttttagaac tgttaatcca cctaatcccg
    12481 aatcaaaaac tcctattggt ttattgttca tctttataca acacctactt tacatttttg
    12541 tgtttaacaa caacttattt atcgctaaaa tttatattta tattcataat taatatatta
    12601 ttacctaaat gattattata gtttatttta cacaaaaaat aaaggagcta tcttaaatta
    12661 gaaaataatt tctacaaaaa gacaactcct ttcactactt aaaaatttaa atttattttg
    12721 caaacacatg atttcctatt ctcatataag gattcaatct atttatccat ttgcaagtgg
    12781 ctatttctgg attccaaaaa tatactgctt tattagttgg gtcagttcca taaagagctt
    12841 cctgagctgc tttacgtgct gagtctgttg gtgccatctt tattgaacca tcaagcacaa
    12901 ctgagaaagc atttttttga tatatgactt cttctattgt gtctggaaat ctagggtcca
    12961 atactctatt tattactaca gctgctacag caacctgtcc ctcatagctt tctcctctag
    13021 cttctcctgc aaccaactta gataataaca tcaagtcatt atcagataaa tttattactt
    13081 ctttctttgc tatatttttt tcttgtttgt ttactttctt aatattttct gttggttcat
    13141 atgcaaatac ctctttaata ggcataaata taaaagtcaa agctaatagt gtagccatca
    13201 gtttactcca cctgtccatg attaaaacca ctccttacat tcttattttt aaaatttttt
    13261 ctctaaatcc ttgactactt caagtacttt aaacttaatt tttactctac ttgttttttt
    13321 tggtgtatct tgttttatta acttatattc tttatcagtt aaaacttcct tcaacttatc
    13381 atctgtagat ttatctatca ctccattaga ttcatcaaca aaacatagtg gagggaacat
    13441 aacacaccac cagttttttc cttcaccttt cccaataata atctttaatg ctttatattc
    13501 accagctgga agaactatat tagaatattg tttagtaggg aaattgctat attgtatacc
    13561 tacctttaca ctgtaattat atccattttt aagaataata tttttactta tttcttccag
    13621 actactatat tcctttttta taatagcttc actttctttt atactttttg aattttgaag
    13681 ctttggctgt aaatatttta ttacttcatc tctaactttt agtttaagtt cttggtcttc
    13741 atctgtattg ctgtttgcta taacatgaaa tcttattaat ttatctttat aatcttttga
    13801 tatattgtct acttttttta cctcaccatt tattactatt gtcattattg atatgactga
    13861 tataagactt aatattaata gtcccaatct aatcttaatt tttctcattg aaaattcccc
    13921 ctcaagataa atttatatca tcaattatgt ccaattaaat ttaatttatt cataatattt
    13981 tttaatacta ataaaaaaag ctatcttaaa atgattacaa tcattttaaa atagcttttt
    14041 aaaccttata ttttataagt ctttactaat catagcctta tctatcataa actgctctgc
    14101 tttttctata tgctctgtag catacttctt ggatagctca ctatcacctt taattatagc
    14161 atcaagtaaa tatttatgct cttcaacaat attatgtgat gtatcataag cagacatata
    14221 ggtaactcta aatctatata cttgttccca caatgagttt attaattgga ttaatttttt
    14281 attattagtt gctttaaata tacattcatg aaattctaca tcaagtttta ataacgcatc
    14341 tacatcggat tccaaagtgc tttttttaaa ttcttctgat atatctttaa gctttttaat
    14401 atcttcatca ctaattcttt cagcggcaag atatgctgct aagccttcta ggcttgctct
    14461 tacttctaat acatccataa tatcctttaa tgacatattt gccacatacg caccttttct
    14521 aggcaacata acgactaaac cttctaattc aagctttctt attgcctctc tcactggagt
    14581 cctacttact cctaattgtt ctgctagttg cacttccatt agcctttgac caggctttaa
    14641 ttttccttct aagattgctt ctcttaaatt ctcaaagact acatctctta atggcttgta
    14701 attatccaag ttcagtttag ttaaattctc cacaaatttc aacccctttt tgactgctat
    14761 taacaacata cacctgttta tattttttta ataattcttt ttttccaatt agagcatctt
    14821 ctttattttt aaatagtcca aaaactgtag gaccacttcc actcatcata gaacctaaag
    14881 cattattttt catcattact tgttttatat cacttatttc tttgtgttta cctatagtaa
    14941 cattttcaag aatatttacc atgctttctg atacagcttt tatatcttca gattttaaac
    15001 actctatcaa atatttatta tttggtcttt ttttaatatt attcaaatct aatccttgat
    15061 aaacttcttt agtagaaacg aataagtctg gtttgcatat caatatattt gtatcacatg
    15121 gtaaaccttt gatatttgtt agcttttctc caataccttg agctaaggca ggtcttcctg
    15181 atatacaaaa aggaacatct gcgcccaaat taagtcctat ttcttttaat tcatcttcag
    15241 ataatctaag ttcccatagg tgatttaaac ctactaatac tgcggctgca ttagaacttc
    15301 ctccagccat ccctgctgct acaggaatat ttttttctat gaatatttct acaccttttt
    15361 ttatataaaa tttattttta agtattttgg cagctttata aacaatatta tcttcatcta
    15421 aaggaatatc taaactagtg ctttttacct ttatttcatc ttcgtctaat tcttttatct
    15481 ttactatatc gtataaatct atcgtttgca ttatcatttc aacaaaatga taaccgtctt
    15541 gcctttttcc caagacatca atggataaat ttatttttgc tctactcttt agtcttataa
    15601 aatccattct atcctccttg tttaaagaat aatgcatcac taacaataat attatactac
    15661 aaaaaataaa ataatagggt attataatac cctattatac ttaattactt taaatttttt
    15721 aatattaatc tactaatacg actttttttt ctaaaataag acacttccca ggcttaattg
    15781 gttcatcaag ttttatatca ttaagttctg caatttcatt ttctgttgta ttgtattttt
    15841 ttgctatatt ccagaaagta tctccttctt tgcatatata tacgattatg ctaggagctt
    15901 tagataaatc atatacacct tggtcttctc ccttgataat aaaattttct gctttcttat
    15961 ctaatgcttc agtaaatctc ttaattttga taatcaagtc tatctggtcc ctattcaaat
    16021 caacttcgac tttatcaata catgctgtat taaacacaga tgctgtatca gttagattat
    16081 ccatttctat atcatgttca aatggaattt cctcactaat tttataaacc aattttaaac
    16141 cttcaactgg gacaaataaa atttcaactt ttatgattcc ttgtataatg cttttatttc
    16201 cttcaatata actattttct atagacatgg ttggacaaac gcttacaata tcttttattt
    16261 gtatatcatc attatcattt cttatgcctt ctctaaccat aaatgtttct tcactacgtc
    16321 ttaaagtctt atttaattga ataggtttgt ggtcaaattt tattatcttt tgaggcgaat
    16381 atgcatcttg taatacttct cttgtaactt catctgtaac ttttacctta caacaagcca
    16441 tacaatctat ttctaaaaga cctgtgttgc tttcactatt ttgcttgaaa atatgattaa
    16501 aatctgacat agacaacaat acttcttcag tcataccatc actaactcca gggacttcta
    16561 caaattgagt aaactcaata cctactctat ccaactctac aagttcaccc tcatatgtac
    16621 aagctaacgg atttatttct aatactccac caataataac tttattgtca gtcactctac
    16681 tttcttttac tttaacacat ggatttaaac ttattataga ttgaatttcc tctgtgttta
    16741 ttgttatagt atctcttata gaactttctg ctttttctat tccaactata tcttgaaaac
    16801 aaatttcctt tctgtgcttt tgtatgcatt ccacttgcgc cacatctttt acaatgtcta
    16861 acctttgctt ttcaaacaaa cttcccctta tattcataag agctcctact ttaatctttc
    16921 tctcattcat tatagtacaa tccatatgct ctacttctga aaataacatg tattccatat
    16981 cttgaataac attgtctttt tctattactt cgtttatatc tacttttccg tctacattag
    17041 aaatagtatt tttatcatct gcaatataga ttacattata attaaaacta cctctgcaaa
    17101 gtatcttccc atctgccatt tcaatttttt taagtgatat gtatccttct gtttttacaa
    17161 tttcatatac atctaatttt ttgtctggca caactgcctc cgcttcaatg aatgtttgaa
    17221 attttccaaa gtctattcta ttgtcaactt taattacatc tttaattaat tccatagtct
    17281 acctccttaa gtattataaa aatatcacac tatatttata tttaaggagc aaaaaaaaaa
    17341 ttacacctat ttttaaatag gtataatttt aacttatttg taatttatct tgatctctaa
    17401 aaacttgcaa ctttacatta gatgttaata aatctgagta actataagat actctaggat
    17461 atccattact ttcatcatct aactttataa caaacacgct tggataaact ttttctagta
    17521 taccctcttt tgtaataatc tgctttcttc ctttattagc tttcaacaat attttctttc
    17581 ctatatgtct ctctaagctt actcttatct tatctagagt ttgaacagta gccacaatat
    17641 caccttcttt aacgtttata tgtttacata atatcacata taaacatttt tgtcaaatgt
    17701 ttaaactaat tattttatat attttttgca aaaatgtcaa tactattttt gaagatatac
    17761 agtataatta tgttaatttt tgctaatgta atattgtttt tggcattgcc catctaaatt
    17821 ttcctcttgt ttcagaacca ccaattccct taacacctgt aatagtctgc tctataacct
    17881 catcaatagg aactactaca tcaattcttg aacaagcaat tttctccact ataaaaacag
    17941 ggtctaatgc atgtatcatt attcttccag gcgaacttgc atagtttgct cctgcactta
    18001 ttattttttc ataatttgat tggcatgctc ctgcgaatat gacaagatta tccaaatttg
    18061 gttgccattt acgtgcttct tttactgttt taacaaaatt tgaagagttt ctatagttac
    18121 tcatatcttc tatatctcct ctttttattg tcattgcatc atgacctgtt attactagta
    18181 tatccggatt atatttttct agtagtgctc ttacttcttt atactgattt tgctctgaaa
    18241 cagctacacc tactgcaggt attccaagtt gagcatatac atccaagcaa atttttaaat
    18301 attctttatc tccatctatc tgtagtactt ttccaggcat tccatatgtg ttaggattta
    18361 attgaagttt tggaactgct ctagtcattc ttttttgtct ttctttagct tttcttactg
    18421 acttatataa taaattttct acatttttat ctattaatat atcttttata tcaggtgctt
    18481 ttactaattc taggtcatca atataagcat ctgctattac tctaaatgca attcccttta
    18541 gtatagctat tttctcatta ttttcatcta caccaaaaga gactatttta aatactatat
    18601 ctttgttatg tgactttcta gctacaatgt ctcctacctt catagttaaa cctccaataa
    18661 atttttatct tagtttatac tatgttcgat ataaaacttt gtttcctaat gtcaaaaaaa
    18721 catttcttat aatacttctt ttatcactct taaagcattt ttatagtata tcttttctat
    18781 atcatcttca ctaaaccctt ctttttttag aggttcatac agtttaccca tctgagatat
    18841 atcttcaatc tcaacttttg aatctattcc atcaaaatct gaccctagag atacaacatc
    18901 tattcctcca acatttacaa tatgttttat gtgtctaacc atatcttcaa gtttactttc
    18961 acttttatca cttaaaaata agtggaagaa gtttatacct gtaactccac ctttatttgc
    19021 taatacttta atcatatcat cagttaaatt tctagaatga ttcatcattg ctcttgaatt
    19081 agaatgtgta gcaattattg gtttagaaga aatctttgca atttcataaa atccaccatc
    19141 tgaaatatga gaaacatcaa ccaacattcc caattcattc atcttatgta ccacttctct
    19201 tccaaaatca gtaagccctt tttctctata ttctacttta ttatgaggaa agctcaattc
    19261 attcacatga ttccaactta ttgtcatcat tctaacccct aagtcgtaaa atttctttaa
    19321 attttctaat ttaccttcaa gtactgcacc ttcctcaata gataaaagag ctgtaagttt
    19381 accttctgat tggtttttca taatttcttc atagttagta gctaaagcta tttcatcact
    19441 attttttttc atttcctcat gaaatttatt tgccatactc atgcaataat caaaaggatg
    19501 ttttacttct tcagtatcaa caaaaagagc aaatgtttga gctaatgaat cccccttttt
    19561 caatctgtct atatcaacag aatatttatt agacttaagt tcacttgttt ctacattttc
    19621 cattaatttt gctatagtat cacaatgtaa atcaagaaat ttcatattta taccccctgt
    19681 atattttata tttatatcta aaaatagaaa tagttgtcct tatagacaac tattgacaac
    19741 tattttttaa tattcttttt aatattactt aatcttattc ttcttttaag ttaggtatta
    19801 cttttttaac ttcttcaatt tgatgccatg ttttatctat attattttca ctaatatatt
    19861 tttttaaatc agttaaagat gtaaaatctt taactgataa attgtcatca tcatctgcaa
    19921 atcttaaaat aaacatgaga ctcccttact ttccacaaca tttcttatat ttttttccgc
    19981 ttccacatgg gcatgaatca tttcttccta tttttgcttc tttaactatt gttttagact
    20041 tcttataagc ctttattatt tcttttcttt ggtctgctgg tagtatatct tcccaacctt
    20101 gtaaattata tagccaatga gcctcaacag ttaccatatt gttatataat ttttcccaat
    20161 ctatttttat agaaacttct ttatcagctt ctacttcttc taaaactata tcttctttta
    20221 agctttcact tattccatct atgaatccca tgaaaaattc attagtagtt ccaaattttt
    20281 cagccaattc acttacttta ccttcaacaa catccatttt attatttaat atttcattgt
    20341 atattccagc ttcaactttc aaatattctt cccagaattt tacttctgct tcttgacttt
    20401 catgattatc acttaaattt ctccattcag tatataaact catacttttt cctcctaagt
    20461 atacctatat ttttatagtt tttatagtat aaaatctttt agttagattg gaagtttctt
    20521 ctttttgatt ataccatatt ctatttaaag ttaactctta tattatatca taattaaaca
    20581 attcttttaa tacttttata gcatactctt ggtttataaa ataagggctt gtcgcaattg
    20641 ttattacttt ggctttttta attaagtttt ttatcttgta aaatttgagt tcaaatggta
    20701 tatagtccat atcacttgaa aatatatcca aatctatatc aagaacaaac tctgattcta
    20761 cttctaaatc aaatccatat agactatcta ttattattaa ttctgaaaat atgttatatt
    20821 ttaaagcagg ttttataaaa cttcccacat ttaaaacttc atttgtatat ctaaatacat
    20881 cttttagatt atttacatct acatcatagt tctcaggttc tctagtatcc ttatgttggt
    20941 ctacatgcac taacttgcat ccttttgtaa actcaccctt taacaaagat tttgcccaaa
    21001 aataaaatgc atggttatga ttgtcaaaca catatatctt tttatttccc aggacatatt
    21061 cgaccatatt ctccaatcct tttgctttta tttccttatt ttcatctact tcattaaata
    21121 ctacctcatt gcctaacctt acatcattta atgtaccatt tattagtttt ggaacgtata
    21181 tgctcttatt ttctctttta tcatatgaaa atacattatt tcctactggt ttttctatat
    21241 aaaatccact atattcataa ttttccataa aactttattt cctccattgt tatataaata
    21301 tataagttat aattttaaaa attaaaatgc acttattgta aatcatatca tctattatag
    21361 aaaacatctt atataactaa atataatatc ttctctcaat atgtattata caataagtgc
    21421 aaaatatatt tatataaaat atactacatt cctataacaa aaccatcagc ttcaattgga
    21481 aggtaactaa ctcttctatt caactccacc tttccaaaaa atactaccca atatggtcta
    21541 aaaatacttt caattttaac tatttcaatc tctgggattc ctcccataaa cctatgagtt
    21601 aatctcatag ccattttatt tgcctttgct tttactcttc gttcgtcctt atcactatac
    21661 tgcacaatat tacaatccac attttttaca acttcatatg taggaaaatc ttgtactata
    21721 ctaccagaac ctgtagaacc attcactaaa atttttaatt catttatatc attagaatat
    21781 ctcctattct tgaaaatgga tttcgttttg tgaattttaa gtgtgataat tttaaactct
    21841 atataatgaa gtctaacttc atgagcatat ttgttcttta atattagctt actaaataaa
    21901 gaattttttc cacttttagc tatctctata gcttcttctt ttgtaattct tataggtaac
    21961 gctcttatat ccataaaatc attctctttc ttattttttt cttgtcattt ttaaaactct
    22021 atatatataa tcaaaaaata ttacaattac aactgccata actccatatg gaattatttt
    22081 tccaactata atatcatttc tagtcaatat accataaaat ataaatcctg ttattaaaaa
    22141 gtacaataat actagaaaac cgtctattcc aagccatttt atttctaaat ctttttttac
    22201 cttagacact aatttagaca tagcagtttc tccttttata gattattttg tgtatttaaa
    22261 ttttcaccga ttctattacc aatcatccat gcaataaatg aaaaaggtat agctatcaca
    22321 agaggctcta actcaaagct ctgaactgga aatattttaa aataaccaaa agtcactcca
    22381 ccaacaatca ttcctaaaat accacccata gatgttcttc ttccatttcc aagaagccca
    22441 cctactatag gtacaaatgc cgaacccatc caaatacttc ctacaaatat acatgcatca
    22501 tatacatttt caaatctaaa tgctacgcta agccccataa ctgctgctaa acatacacct
    22561 attctagtaa actttactaa ttgtttctga gataactctt tttttgtaat tcttccatat
    22621 atatcatttg ctaatgtagc cccagctgct agataatagc tatccaatgt tgatattata
    22681 gaacctaaaa ttcctattat aaatattact cttattcctt gtggtaatgt atctaaaact
    22741 aaagttatat atccagcagc tggagccatt tctggataag tagctcttac tataagtcct
    22801 gtcatagtta acaccatgtc aaaacatatc catagtgaaa aacaggttaa taaggctctt
    22861 cttccaacct taggagaatc tgctgctgaa aatctttgat aaaaagttgg gtctgaataa
    22921 gctccaaaag aatatacaat taacatcaat gctttcatag ctgacatacc ttttgtaggg
    22981 tgcataagct gtggtgcatc tttgagtgct tctgtaagac cttgaaaccc tcctatttgt
    23041 tcaaattgac ctggcataac catagttaaa ccaaatacca tacaaaaaaa caataccata
    23101 tctgtaatgg caactcccat aagcccacca agtactgtaa gtacaacgac tactcctacc
    23161 attattaatc ctattaattc tttatctatt ttccatgcta cgttacctat agttcccata
    23221 gcagttattt ctccaagttg tgcacaatat ataaacatca aaattgcccc aattatagca
    23281 acatgcttgc cataaaaact ttccagtaaa tcaggaactg ttatattggt tcttatatga
    23341 atccttggac caacccacaa tgctaaaggc attctcccaa tatgtgcagc tattgaccaa
    23401 actgcccatg cagatattcc atatatagcc gcatactcta cactacctac tgtaccaact
    23461 cctccatacc atgatgcaac caaagttcct actattaagg accatggtaa acttcgattt
    23521 cctaggtaat aactttccat atcttttaca tttctagaaa aataataacc tattcctaat
    23581 aatataaatg caaatataac tataatccca ttttcccaaa atgttaacat atctaatctc
    23641 cacattctta tattttaatt tttaacttta atatctaaat atgaacaact ctacccattt
    23701 ccataaaaat aggtagagtt atataataat tataagtatt taagtaatct tgttattaag
    23761 caattttatt gtttaaacct ttattttctc ttttatttat tttatttaaa gcataagtag
    23821 caaatatccc tataactgca catactacag ataaaataaa tatatatgta tatcctgttt
    23881 gtccatattt atctaaccaa ttacctgcaa tagtgtaata aaacgcatct ggagaaaatc
    23941 ctataaatga agcaaatcca actactgctc ctgtagtttc taaagatata ttagactctg
    24001 aaacagatgc aaagtatata cctctaaatc caaataaaat tacacttagt attatcatgt
    24061 ttattacagc tatccatata aatcctgtac tcttaggaat aaccaagtaa gcagctgtac
    24121 ttactgtcat taatataaat cctacaaaca ttactcttat agatgaacca actttatctg
    24181 ttataacacc agcaactgga gatgctccca tttttataac ataagttctt attattgata
    24241 aagcagaaac tagagtcatt ggcattacat aaacttctga taagtatgga cttaaataag
    24301 ttaaacttga gaatactaaa taagttgaaa atattataag accaattaac caagcctttg
    24361 gtattgtaac tacttttaac aatgacttaa tacttaatcc ttcatctgat gctccactat
    24421 cttttttctc ttccactaag aaaaatagaa gtataccaca tattatagat gctgcagaat
    24481 ataaccatac tacatatttg aatcctactg ttatttcagc aaatttacca aagaaataaa
    24541 gacctataaa agaaattaat gtacctgata cacctgataa cccttcataa aaaccaaata
    24601 ttcttccttg ctctgactca cttccttgca ttctaacaac cttaacagag gctgagaaga
    24661 aggttaatat tgtcgtaaat ccccataata caaatattaa aagtatcata ctatatgatg
    24721 ggaatgtaga gaaataaaat ccaagtatcc ctgaagcaat taaagaaaaa gttattaatt
    24781 ttctaactga gaatctatct gcaaaccatc ctcctataaa ataagatacc attcctacta
    24841 atccatataa actcaataag ttccctattt gtgtatgact taatgctaaa gcttgttgca
    24901 ttgggtcata aaatgatgat ttcatatatg gtaaactata catagctgtt gttccaaatg
    24961 ccaatatgaa tattatcaaa aatcttttaa cattattttt gctttccatt gattctctct
    25021 ccttaaaatt ttattgttta taatctcatt catataattt tattttaatt tttctattgc
    25081 ttgtttaaaa tcatttatta tatcatttga agattctaat cctacagcta ttcttattag
    25141 attttcccca ttcatttgaa ctaatgtatg ctcttcccct aagcttgttg ctattgttgc
    25201 aagctctaaa gagttcatta atcttctact agcttggtct cttgtcaatc cactgatacc
    25261 atccttaagt ttaaatgaaa ctacgcctcc acctaaacca ttcatttgag ttttacaaag
    25321 ctcatattga ggatgtgatt ttaatgctgg atactttacc aattccacat tctcttcttc
    25381 ctctaaaaac tctgctactg ctaatccatt ttcacaatgc tttttcatcc tcatatctaa
    25441 cgttttcata ccccttaaaa tcatccatgc actcattggt ggtaaagatg ctcctgtaaa
    25501 acaacataaa cttggccatc taactaaatc tattaattct tttgaacctg ctactgctcc
    25561 ccctaaagta tctccatgtc catttataaa cttagtaaga ctatgtatta ctaaatctgc
    25621 acctaaatct attggtcttt gtacaattgg tgtcccaaaa gtactatcaa ctatagctaa
    25681 gcttttattt tcatgagcaa tttctgctat tgtttttata tctactaagt ctaatgatgg
    25741 atttaaagga ctttctatat aaactatttt agtatttggt ctcattgctt cttttacatt
    25801 ttgtggattt actgcatcta caaatgtaac ttcaactcca aatttagata acaactcaga
    25861 catgaataat ttagtgtgtg aaaatattcc atctgaacta actatatggt caccactttt
    25921 taccaatgct aatatagtag atgttattgc ccccatgcct gatgtactag atatggcact
    25981 ttctgcattt tcaagcatag ccaacttagc ttctaactct gtaagagtag gattacactc
    26041 tcttgtatac acatatcccc atgtcataca caactcttca aaatgctcta ctgtctttgc
    26101 agcaaatgtt gctgtttgac atattggtgt agccaatgca cctgttgttg ggtctgcact
    26161 atgtccccaa tgtattattt ttgtctctaa atcttgatta atattgttac tcattttgat
    26221 ttcctcccat tttgacttat ttatacaacg ttttccctgc caaatttcat caatttagct
    26281 tagtcttaat acattttttc aagtatactt ttttatttgt tataatttct aaaaatatga
    26341 cacttatttt gtttttttac agttctaatt ataaactcta tagtaactat agagtcaata
    26401 tgtttttgat atttttttta caatgtttaa aatagtcaat acaaaaatag gtatatctta
    26461 gaataactaa agtcatccta agatatacct atttttatat cacaatataa ataattacat
    26521 ataagcatta gtttttttat cataaataga tttttcaaga tttatcattt tttcataaat
    26581 atattatagt taaatatttt aatattacct aacatctaca ataaaatata ctcttaaaat
    26641 aaaattatat aattaaaaat actctataaa agctaaaaag tttttctatt cgctacatct
    26701 aaaaataatt agatagaggt ataaatgtat ttagtttatt tatacctcta gtcttttata
    26761 aattatatct ctactaaatt gattttttag cacgaataag tgtgctcttg gagataccag
    26821 tcatcttcac aacctctcta tatggcttag aatttaataa ccccaatgca tattgtatct
    26881 gttgattgct gtatttcttt ggcctaccat ctttaaagtc ctttttcttt ttagcaaatt
    26941 cttttcctaa ttgagtacgt tctattatca tatttctctc aagttctgca actgcaagta
    27001 atgtagtcaa aaagaattta cccattgaag tattttctaa aaggcctata tttaaaatat
    27061 gaacacttac ttctttagca aataaacctt ctattagttg tattccttct gatgtacttc
    27121 tagctagtct atccagctta gttgcaacaa gtaagtctcc cttttttaga gatgatataa
    27181 ttttttcaaa tttaggcctg tttgtagtag tgcctgtata agtttccttt attattttag
    27241 catcattata tttattcaaa atttcttgct gttgctgata caaactgttg ttgtctgttt
    27301 gattttttgt acttacacgg caatatccat atataataat catcacttcc taacataaag
    27361 attttgtaca gaagttaccc agaagcttgt ttttacttat ctttatccat tttactaaaa
    27421 cgatttttgg gtgtcaatac tgttaacttt tgacaccata ataattcctt tataatttgt
    27481 ttgactatgt caaaaattac caatataatg aaattaatga gtttatacaa attggaggaa
    27541 ataatatgaa tagtctgtta aaagaagcaa tagatgaatt aaaaaacatc cattgcgggg
    27601 aagttttttt attaaaagaa ttgttcaaag gctatgaatg gaacaggttg gagatgaata
    27661 tcagattgaa attaggaaaa ctttttttgt atgaagtggt aactaatccc aatttaaatg
    27721 tagaggtcat caataaaaat tctgcttatc agcaaatgta taaaaaaaaa tagtgactta
    27781 aatgtatata atagtataat cttcttattg attcaaaagt taaaactttt aatacaaaac
    27841 aatcattcta ttttaacatt tatcaatatg tctaaattta tataattaac acatacgaat
    27901 agtattgttt aaatcacatt atttaagata gataaatctt aaccctactc cagcataaat
    27961 actaaaatag ggcttagaat tatctatatt tttatcccat aaaatgctta gctcttcctc
    28021 ttgattgaac atatatatct ggggaaatac aatagaagcg taaatttata aaatttccaa
    28081 agtcagaaaa cttcagttca aacttaggta taccaaaact ttcaggtgct atttcgtcaa
    28141 cgccaaaata gatgataatt ccattatctg taagataaaa tgcttggtct tcaggtataa
    28201 ctaactcaac attagaataa tacatttctc tattttcatt tatttgagat ttaatgtaat
    28261 cagtaattat attaatataa tcaactccat ctctaaaaat gtcattaagt aaaagttctt
    28321 ttccagttgt caaatctata ttgtaattat tcaatgtatt gtatttaggc ccatagttat
    28381 tagcaaaccc cataaggttt aatatcatac tcagtacata atttttatta aaagtaactg
    28441 cgtaactggt agatacccaa tagctttgtt ttggaagtga attttgagaa gctgcgttat
    28501 tatattctcg ttcttcttcc tgtaagccat ttttaaaatt atttacagtc attcttacag
    28561 cattgtttat gttatttaaa ataactgggt ttacatacgt ggattgattt ctaatattgt
    28621 atataacagg atactttaca tcatatgaaa aatacttact tctttcaaaa tcttcattaa
    28681 attgtacata taatatatta tatgtacagg tatttgtaga ttgataattt tccattaaaa
    28741 tcctccccct tttataaaaa aatcagatac aatataatat gcttaaaaca gtaataatgt
    28801 taatggtaaa tgtaaagaat agaaagtatc taaaacagca aaaaatgact aattttgtgg
    28861 tataatacat aggattgaga gttattttat aggaggaaaa atatgttatt attagcagat
    28921 aaatggaaag attatgaact tatagatatg ggaaatggag aaaaattaga acgttgggga
    28981 agttatgtac ttagaagacc agacccacag gttgtatggc caatggaatc tgaatgggct
    29041 ttatggaaaa accctcatgg acattatcac agaagcaata aaggtggagg tcaatgggaa
    29101 cacaaaaaaa gatatccaga acaatggact ataaattata aaaacttaaa gtttcacatt
    29161 aaaccaactg gctttaaaca tactggatta ttcccagagc aagcagctaa ctgggattgg
    29221 atgatagaga aaataaagaa agctaacaga cctataaaag tgttgaatct ttttgcatat
    29281 acaggtggag ctactgtagc atgtgcatct gctggtgcag aagtatgtca tgttgatgct
    29341 gcaaaaggta tggttaattg ggcaaaagaa aatatagaat tatcaggtct taaaaatcaa
    29401 acagtaagat ttatagtgga tgatgttgta aagtttgttg aaagagaaat aagaagaggt
    29461 aaaaaatatg atgcaataat aatggaccct ccttcttatg gaagaggtcc taaaggagaa
    29521 gtatggcaaa tagaagaaaa gttatacagt tttgtagatt tatgtatggg agttttatca
    29581 gataaccctt tatttttctt aattaattct tatacaacag gattttctcc aatagttctt
    29641 gaaaatgtat tgtctacaac aataggtaaa aagacaaata caggagatat atacggtcgg
    29701 gaattaggaa taccagcttc cagagatggt aagatacttc cttgtggtat atttggaaga
    29761 tgggagaaaa aataatgata aaagtcatat atgaagataa ccatttatta gttgtagaaa
    29821 aaccagttaa catattgtct caaggagata atactaatga taaagatatg gtaaatcttt
    29881 taaaagacta tattaaaaaa aagtataaca aacctggaaa tgtatatatt ggtttaatac
    29941 atagattgga tagaccagta ggtggagtta tggtgtttgc aaaaacatca aaagcagcat
    30001 ctagattgtc tgagcaggta agaagtaaga ctttcaaaaa aacttatcta gctgttgttc
    30061 atggcaagat aaaatctaac tcagatatac taaaagattt tttatataaa aataaaaaaa
    30121 ctaatatggt aactgtagtg aaaaaaagcc ataaagatgc caaagaagct gaacttaact
    30181 ataatgtatt agattttaaa gaaaatctaa gtttagttca agttaattta aagactggtc
    30241 gttctcatca aataagagtt caatttgcaa gcagaaaaca tccattatac ggagaccaaa
    30301 gatatggcaa agaaataaat aagattggac aacaaatatc tctttggtcc aataagatag
    30361 aaatttgcca tcctactaca aaagaaaaaa tggaatttac atgtcatcca cctgacaaat
    30421 atccatggaa tatattttaa atctcaaatg aggtgattat atgagtaaaa aattttcaat
    30481 aggagaaatg tctaaacttc ataaaataag catacagaca ctaaggtact atgaccaaat
    30541 agatttattt aaacctgttc atgttgacca agaaagcaat tacagatatt actcaattga
    30601 ccaattttca caattagata ttataaaata tttaaaatat ttaggtatgc ctttaaaaga
    30661 aattaagtca aagcttgatg aaaataatga agaaattcta aaattactaa caaataaaat
    30721 taatatagtg gatgaaaaaa ttaaagaatt agagcttata aagaaggtac taagcacaaa
    30781 gaaagaaact atggaaaaag gtatgaaagt agatgaagtt ggtaaaatag tgagaaaaca
    30841 tattccacga agaagtatct tatctataga gtatgaagaa ggattaagta gtgaagaaac
    30901 tatggaactt tccagaagac aaatagcaaa tatattagaa gataatatat ctgtgttcta
    30961 tggtggagta agtggacttg tatctataga taaattttta aaagaagata aagttattta
    31021 tgacaatagt tttgttactg ttgaaagaga cttatttgat ataaaatcac aaaaacatat
    31081 aacagaaatt tcatctggtg attttatttg tatgacatat aaagggcctt acaaagaaaa
    31141 ttataccgct tttaagaaac tggttgatta tgttgaatat aaaagaatac ctattgaaaa
    31201 ttatatatat gaaataccta taatagaccc tctgtctaca aacaacgaag aggaacttct
    31261 aacagaaatt caaatacaag ttaaaaagag agaaattgac ttaagatgtc tagtgtagta
    31321 atctagttta cttacacatc ttaatatagt tgtctattat caacatatga tttttctaga
    31381 tagtgattcc taacagtgag attttcaaaa aataggagga aatatggcta atgctaattc
    31441 cctcctattt ttaattaagc catatcattt taattaagcc atgtcatcac gaagtgcatc
    31501 aataatattt tcttttttta tcttattaat agtatacaac attgtaataa atactataaa
    31561 taaaacacta aatatactaa ttccaatact aaaccatgga atcacaaaat caatatcatc
    31621 tgtaaccatt actttatgaa ttagccatga agaaattact gctataggaa gtccaaagaa
    31681 caatgctctc atgccataaa aggcacactc aaaattcatc atcttttgga aatcatgctc
    31741 agacattcct acagagcgaa gcatggcaag ttctctacga cgtaacttaa tattagttga
    31801 aattgtatta aacacattgg caatcgaaat cagagaaatc ataattatga aagtataagc
    31861 aaaaacattg gcaataaaaa tgtaattacg gttctcatcc atcattccat acatattgta
    31921 cagattgtac tcattcgtaa ctccctcact ctcaataatt tgctccattt ctgcaactga
    31981 ctgagtagga tttttcgatt gaaagcttaa actttcaatt cctgtagaaa caccaggaat
    32041 ctcaaactta tctttggctg aataaggagc caatactctg aagcttgctt tcttcacatc
    32101 tgaacttctg attcctggag gagaatcagg tggcactact ttgacaaatg aaatattcac
    32161 attttgtctc tcaggtatct ccaacttgcc attagtttca ggagtgaggc taacattcat
    32221 agaagaactg ttgaaaatat caataatatt attatccttt gttaccatag aaacagcaat
    32281 cagttttgca ttttgtccag tatattcttc tgctggtaat ccaagacttt taataatatt
    32341 cagataagtt ctatcatcaa gaaattgcat atctactggt aattcaagca tttcactcat
    32401 agaatgtgaa ccctcatagt tcaaataatc gtctgtaagc tcacttactt tggcaacaca
    32461 tgaataggtc ataagtttct gatacaaacc ttcgtaaaca ccatttgcag tttttagctt
    32521 atcgtaaagc ttcaacattt cgttgtcttt cataccttgt gtagaaaaaa taatatcctg
    32581 gtcagtaacc tctgacctct gatttgacat ctgttttagg tctgctacta aagcacttgt
    32641 agatataaat agaactatgc ttaaaacaag cgacaataca atacttcgat aacgtttctt
    32701 gtttctttta aagtttttca tagcaagaac tccctcaaga ccataaactc gctgtgttag
    32761 ttttgatgtc ttcacagatt tagattcaat ttttacctca tttgtctgac gaatacactc
    32821 catcactggt gtgttggcag cctttcttgc tggtatgtaa gcagaaatta agattgtaac
    32881 catacttacc actgctgcaa cgatgattgc agggatagac aatgttaatg tcaacggaac
    32941 gtttacatag agaatatttg caaagttttt agatatgaca gaaatcacaa gtccaatact
    33001 gactataccg attgcaacac caattggtat accaatcgca ccaatgcaaa ttccttcaaa
    33061 tagcaccgaa tttcgtaact gttttgctgt agctccaaca gatgaaagta ttccaaattg
    33121 atgtgttcgc tcgttcaatg acatgttaaa tgaattatat attagaaaaa ttgagcctag
    33181 catgattatg gcaagcacaa tgccaccaac cgagtacaaa aacatattga acaaattgtt
    33241 atcagagaca cctatgaatc gcaacacatt gtcattgaaa atggaagcat ggtctttagt
    33301 gctacttgtg taagagcgaa tctgatgtgg atttttcaat gtgacaaaaa ggctaagact
    33361 gtcagtcttg acttgtttat cttctttggt tatcaaggta tatcctggtg cagaagattc
    33421 ttcaaatacg ggctttctac agatacccac aactgtataa gttttcttat cctgtggcac
    33481 aaaaacttca ctcccagcag tgtatgggtc actctgacca agcttttcat tttcactcat
    33541 tcgtttccct acatccaaag aaatagtatc ccctactgca aatttgacac caccatctgt
    33601 cgcaacttta cttgaaacaa gaatctctct actattttca ggtaatcttc cagaaattaa
    33661 agttataggc aaagtatcaa aagcttcttt gctaaatcca gcaataaaaa gatatggttt
    33721 atctggattt ctgttacctt caagtgttgc atagccaata ttttcaaatg ttgcagtatt
    33781 tgcaacttca tcattgactc tttgttcctg tacaaaagaa gaatccacat ccaaaaactg
    33841 gacatgccaa tcaccatatt ttataatagc tccatttatc atatagttca tcaaagaaac
    33901 tccaaaggta gtaactgctg taatcatagc agctgacaga acaactccta taattgttac
    33961 aagagttcga gttcgattct ttttcaatcc ttgcagagca actttgttaa aaatgttcat
    34021 taacgattca ccttcctctc atcacgcact acttttccat ctgaaataca gataattcta
    34081 tctgcctgta aagcaatgtt ttcatcatgt gtgacaataa tgagagtttg gtgatattta
    34141 ctatggctct ctttcagtaa ctggataatc tcttgtccat ttttactgtc taagctacct
    34201 gtaggctcat cagcaagcat aactgctggt gcattcatca atgcacgacc aatggcaaca
    34261 cgttgctgtt ggccaccaga aagttgattt ggtaaatgtg tttttcgttc ttttaaacca
    34321 agtaattcta acaagtcatt taaccgttct tgattgacct tacgcttatc cataagtata
    34381 ggcaatgtga tattttccac tacatttaat gtgggtatca gattgtgaaa ctgataaatc
    34441 aatccaactt gtcgcctacg aaaaatagca agtttttcat tgctttggcc atatacatct
    34501 tgcccctcca gatatatctt tccacttgtt ggtacgtcta ctcctgcaat ggaatgtagt
    34561 agtgtagatt taccagaacc agaagaacca atgattgcag taaactcccc tttttcaata
    34621 gtaagtgaga catggtcaag tgcagtaacc ttattttcat tcttaccata aaccttacat
    34681 aaatcttcaa tctttaaaaa cgccattata taagtctcct ttcatatttt ataaatatat
    34741 catagaactt tcaacttaca tttaagtgac atttaaatga gagattcgtc actttggaaa
    34801 acgaataaca aatattgcac caccttgtgg gtgattttgt gcagtaattg ttcctccttg
    34861 tctcataata atcgtcttac aaagtgccaa tcctattcca taaccggctg tgcttgaatt
    34921 tttcccacga taaaatctgt taaatagaca tggtaaatct tcttttttaa agcctgctcc
    34981 gttgtcatga atagtaagtt gagtaaacaa aaagttgtcc tcacaaatga tatcaatttt
    35041 tccattatca cccacactct ccatgcagtt tttaaatata ttttgaattg cttctgaaag
    35101 ccaatttaaa tcacccaaaa tccttactcc ttttggtata tcaatatgta aagtgatatt
    35161 gtgcagttcc atgggaatca aaagttgatg aagtgcagaa ctaatcaaat tattcacatc
    35221 aatttgttct ttttgaaaga ctacaatacc tgcatctaaa cgtgataatt ttaaaaggga
    35281 agtaatcagc cagtccattc gtataagtaa ttcctctgtt tctcgtatca actcttttct
    35341 ctcatcttca tcagaagtat tctctaacag tgacaaaata agagttactg atgtaagagg
    35401 agtgcgaagc tggtgggcta tgtcagctag tgagtcagca aggtgttctt tttctttttt
    35461 cagtgcgtaa ttttgctctc taatgcgtaa cgtcatttta gttatttcac tttgtagaat
    35521 agaaagctca ccctcatctg attcagcaat atacagatgg ttagcattat gaagcacaag
    35581 attaatttgt tctgaaatct gtgcaatact tttgtatcga tttttagtaa acacaaaaaa
    35641 tgctattcca aaggtagtgg cagaaacaat agaaagtatt ccagtcataa tactgattgc
    35701 aaatcccagg gctacagtga gaatagtaat taataagaat aaaatagcaa attttcgaaa
    35761 ctctctattt cgaaacataa cttcccccca atctataccc tgtcccacga acagtcaaaa
    35821 ttatttgtgg acttgaaggg tcatcttcta tcttctctcg caagcgtttg atgtacacag
    35881 tcaatgtgtt gtcattaaca aactcacctg ccgcatccca caattcatca agtaatctta
    35941 ccctcgtaat aatactttta ggattgctaa taaacaccaa taacaaacgg tattctaaag
    36001 ctgaaagaaa aacctcattg ccattctttt tcacaacacc agttgctgta tctacataaa
    36061 gcccacaaat ttcaaaagtt gacccaaaac gcccactttt tcgtagtgct gttctaattc
    36121 gcgcaatcag ttcacgtgga cgaaaaggtt tagtaatata atcatctgca cccatattca
    36181 gtccagtaac aacacttgat tcatcaccgg aagctgtcaa aaagataaca ggaatgtctt
    36241 gcttttcttt gatttctgtg caaatcgtaa acccatttcc atcaggtaaa gaaatatcaa
    36301 taagtgccaa atcaaattta ttcttagcaa tctctacaag tgcttcactt cttgtggata
    36361 catgggttac tataaatcct tctgaattaa gcaaaagaat aagatttttg gcaattgctt
    36421 tatcatcctc aactaaaaat atccgtttca tttactttca tcctccttta ttttattagt
    36481 tttatatatt ccagcagagc aatagtacac tactatttta cttatcatta cacaattttc
    36541 agtagtttct tttgttctta tataatccat gcataatacc tccattgatt tattatattt
    36601 cttataacat aaatacacaa gataagacaa aaaacataat gtaagtgcaa gttttttaac
    36661 tcactaactt ataaacatac actcctacta acactacttt cttgatgaca catcttacaa
    36721 gcttatattc atacatcaat gaaaaattaa atttatccat ttattaaaat aaaaggcaat
    36781 agcttctata gctaatgccc tttatcttaa aatgatttaa atactataca atttttataa
    36841 atggctataa ataaactcgt atagaatata aaatttatct acagtctttt gttatacaaa
    36901 atttataacc tcttatagaa caaatttacc actttgcaaa cttatcactt ttactcagct
    36961 atattataaa cttttaattt atctttgttt aaatgtttct tcttaacttc tactaaagct
    37021 tttagcgtat ccaaagatgt cattatttca gtagaaaatt caatggctgt tcttctcatc
    37081 ttaaatccat ctcttgtaga gtcatttcct ttagttggtg tatttattac tatatcaact
    37141 tgtttatttc ttatagcatc taatatgttt ggtctaggtt cttcaattct atttactata
    37201 tcagcctcta ttccattttc tcttaaactc ttcgctgttc cttctgtagc aacaaaagta
    37261 tatcctaatt ctttcatatc ttttgctatc tctataaact catctttatc atgattattt
    37321 acagtagcaa gtactactcc tctttcatca gacatatgtc tacctgctgc caagaaccct
    37381 ttatataagg cttcttctaa attttctcct acacctagga cttctcctgt agacttcatt
    37441 tcaggtccta gacttacttc aactttagat aatttagaca ttgagaatac tggtactttt
    37501 actgatacta attttggctc tttgtaaact cctgtaccat atcctaaatc ctttaattta
    37561 gctcctaaca tacacttagt agccaagtca actattggta ctccacttac tttacttata
    37621 tatggaactg ttctactagc tctagggtta acctcaatta tatataattc attttgaaat
    37681 tctataaact gtatattaac cataccaagc acattaaggt ctaaagctat ttttttagtg
    37741 tattctacta tcttttcttt tatttcatca gatatatttt gacttggata cattgttgta
    37801 ctatctccag agtgaactcc agctctttct aaatgctcca ttataccagg tatcaatata
    37861 tcttctttat cacatatagc atctacttct atttctctac cagttagata tttatctata
    37921 agtactggat ttttatggtc tctatcaaat gcatcatcca aatactgaat tagtttttct
    37981 tcgttataag ttatttccat accttgacca ccaagtacat aagatggtct aactaatact
    38041 ggataaccta actccttagc aatttcaaca ccttcattag ttgtccatac accttttcct
    38101 tttggtctat ttatatctag tctctctagt aaatcatcaa atttttctct atcttctgct
    38161 gcatctatat ctctaaagtc tgttcccagt ataggtatat ttttttcatg taaaaactta
    38221 gccaatttta tagctgtttg tccaccaaac tgtagtatta caccttctgg tttttctttt
    38281 tctattatat ttaaaacttc ttcttctgtt aatggttcaa aatacaactt atcagatgta
    38341 tcaaaatccg tacttacagt ttcagggtta ttgtttacaa ttatagtttc tattcccatt
    38401 tttcttaaag attttacaca gtgaactgaa caatagtcaa attctattcc ttgacctatt
    38461 cttattggac cagaacccaa tactacaact tttctcttat cactaactac tacctcatca
    38521 tattgttcat aagttgaata gtaataaggt gatagagctt caaattctcc tccacatgta
    38581 tcaaccattt tataaactgg ttgtatatta tataaacttc tcaattcata taatttttct
    38641 gggcttatct tcattaagtc agatatacct ttatcagaaa atcccttttt ctttaattca
    38701 tgtaaatatt ctttatctaa gtcttctatt tttagaactt ttaacttttc ttcttgttct
    38761 actatccatt taaatttatt tataaaccac ttatcaacac cagttatttc tgatatcatt
    38821 tctactttat agcctcttct tatcatttca gctaagtcaa atagtctctc atcatcaggt
    38881 actactactc tagcctttaa ttcttctaat gttctacctt ctgatggagc atgaaccaat
    38941 gaatattttc ctgtttcaag tgatcttata cctttaagaa tagcagcctc aaagttactt
    39001 cctatactca tgatttcacc agttgccatc atcttagttc ctaattttct attggcatgt
    39061 ctaaatttat caaatggcca ctttggtatt tttactacca cataatctaa tgttggctca
    39121 aaacaagcat aagttttttt agttactgca ttttctatct catccaaagt ataacctaaa
    39181 gctatcttag ctgctacttt tgctattggg tatcctgtag cttttgatgc tagggctgat
    39241 gacctactaa ctcttgggtt tatttctatt atcgcatatt ctaaactatg tgggtttaaa
    39301 gctatttgaa cgttacatcc accttgaacc tctattgcat ttattatgtc tattgatgcc
    39361 tttctaagta gttgatactc ttcatcactt agagtttgac ttggagcaac aactatactg
    39421 tctcccgtat gaactccaac tgggtctaca ttttccatat tacatacagt tatacagttt
    39481 ccatttccat ctctcattac ttcatattct atttctttcc aaccttttat actcttttca
    39541 agaagaactt gtccaacagg acttagctgt agtccatgtg aaagtatttc tcttaattct
    39601 tcctcagtat cagctatacc tccaccagtt ccacctagcg tatatgctgg tcttacaact
    39661 actggatatc ctatcttatt ggcaaactca agacctgctt ctaaatttgt aactatatca
    39721 ctaactatta ctggttgatt tatttctttc attacttctc taaataaatc tctatcttcg
    39781 ccctttttta tagattctat agatgttcct ataactttta cattatattt atctaatata
    39841 ccagcatcat gtaattctac agctaagttt aatcctgtct gaccacccat acctgcaagt
    39901 aaactgtctg gtctttcttt ttctattatt ttttctataa attctattgt taatggttct
    39961 atatatattt tatctgcaat ttctttatca gtcattatag tagctgggtt actatttact
    40021 aatacaactt caattccctc ttcttttaat gcttggcagg cctgtgttcc tgaatagtca
    40081 aactctgcag cttgacctat tattattggc cctgacccta atactaaagt tttctttata
    40141 ctatctaatt taggcataac tctttctcct tatctaaaca attttcacag ttcttatctt
    40201 catcatctat acaatcaact ttaattactt tattttatct taagttcaaa tttactcatc
    40261 aatacatcta tttaatacat ctatgttaat taaaccaaac tttttgattt aagttattct
    40321 tgttttttag ttagctattc taatgttgtt tattttacta attctaaaaa tttatcaaaa
    40381 atatagtcat tatcttttgg tcctgggcaa gcttcaggat ggtattgaac actgtataca
    40441 tctagcttct catgtcgcat tccttcaaca gtattatcat ttaaatttat atgtgtcact
    40501 ttcatttgtt caggaacttc tgaaacataa taaccatgat tttgagaagt tataaatact
    40561 tttccttctt ctaaatcttt aactggatga tttccacctc tatgcccaaa tttaagttta
    40621 gcagtttttc ctccaagagc taaagctaaa agttgatgtc ctaagcatat tccaactata
    40681 ggttttttac caataagagc ttttatattt tctataacat cttctaagtc ttctgggtct
    40741 ccaggtccat ttgacaagaa tattaagtca gggtttatac tcaatacatc ttctggagat
    40801 gttgtagctg gaaatattgt tatatcacat ccacgagcta taaatgatct taatatattt
    40861 ctttttactc caaaatccat aacagccact tttggaccat ttccttttat atgctctact
    40921 tcttttcttg taacagttct aactgcttct gtatttgaaa acttatccaa tttatgttta
    40981 acatcttcta atttactgtc ttctaatgta attatacctt tcattgttcc attatttcta
    41041 agtatcttag tcaaagctct tgtatctatt ccttcaagtc cgataacttt attttgcttt
    41101 aaatattggt ctatatcaat ttcacatcta aaattacttg gagaatcact tttctccctt
    41161 actatgaatc cctttacttg aactttttca gactctgctg atgataaatt tataccataa
    41221 tttcctacaa gaggataagt catagtaact atttgaccat agtaagaagg gtctgttaaa
    41281 acttcaccat atcctatcat cgaagtatta aatactactt ctcctacact ttcttctaaa
    41341 tatccaaatg cctttcctat aaaaactgtt ccatcttcta atattaacct tgctttcata
    41401 ctacagtacc tcctataatt ctctttagac aaactaaaga acattaacta aatataagct
    41461 tttaaatatc tatatctatt tttcactttt acattctagt tttatactta tttacattta
    41521 ttttttatct ttttctatta tactacaaat ttcttaaaaa aaagaagtta tctcaaaagt
    41581 aaaaactcct agaaaaaata acctcttttt tttatttaat aattaaattc ttaaactata
    41641 caatcaactt aatttctatt cagctatatt gtaaactttt aactcatctt tatttaaatg
    41701 tttcttctta acttctacta aagcttttag tgtatccaaa gatgtcatta tttcagtaga
    41761 aaattcaata gctgttcttc ttatcttaaa tccatctctt gtagagtcat ttcctttagt
    41821 tggtgtattt attactatat caacttgttt atttcttata gcatctaata tgtttggtct
    41881 agattcttct actctattta ctatatcagc ctctattcca ttttctctta agctattagc
    41941 tgttccttct gtagcaacaa aagtatatcc taattctttc atatcttttg ctatctctat
    42001 aaactcatct ttatcatgat tatttacagt agcaagtact actcctcttt catcagacat
    42061 atgtctacct gctgccaaga accctttata taaggcttct tctaaatttt ctcctacacc
    42121 tagaacttct cctgtagact tcatttcagg tcctagactt acttcaactt tagataattt
    42181 agacattgag aatactggta cttttactga tactaatttt ggctctttgt aaactcctgt
    42241 accatatcct aaatccttta atttagcccc taacatacac ttagtagcca agtcaactat
    42301 tggtactcca cttactttac ttatatacgg aactgttcta ctagctctag ggttaacctc
    42361 aattatatat aattcatttt gaaattctat aaactgtata ttaaccatac caagcacatt
    42421 aaggtctaaa gctatttttt tagtgtattc tactatcttt tcttttattt catcagatat
    42481 attttgactt ggatacattg ttgtactatc tccagagtga actccagctc tttctaaatg
    42541 ttccattata ccaggtatca atatatcttc cttatcacat atagcatcta cttctatttc
    42601 tctaccagtt agatatttat ctataagtac tggattttta tggtctctat caaatgcatc
    42661 ttgtaggtat tgagataatt tttcttcgtt ataagttatt tccatacctt gaccaccaag
    42721 tacataagat ggtctaacta atactggata acctaactca ttagcaactt caataccttc
    42781 gttaagtgac catacaccct ttccttttgg tctatttata tctagtctct ctagtaaatc
    42841 atcaaatttt tctctatctt ctgctgcatc tatatcatca aaatcagttc ccaatatagg
    42901 tatatttttt tcatgtaaaa atttagccaa ttttatagct gtttgtccac caaactgtag
    42961 tattacacct tctggctttt ctttttctat tatacttaaa acttcttctt ctgttaatgg
    43021 ttcaaaatac agcttatctg atgtatcaaa gtctgtactt acagtttcag ggttattgtt
    43081 tatgattata gtttctatgt ccatttttct taaagatttt acacagtgaa ctgaacaata
    43141 gtcaaattct ataccttgac ctattcttat tggaccagaa cctaatacta tcaccttttt
    43201 cttatcacta acaactacct catcatattg ctcataagtt gaataatagt aaggagatat
    43261 agcatctatc tcacctgcac aagtatcaac cattttatat gctggtttta tattgtatag
    43321 acttctcaat tcatataatt tttctggact tatcttcatt aggtcagata tacctttatc
    43381 agagaatccc tttttcttta attcaagaag atagtcttta gttaaatctt caattttcat
    43441 aacttttaat ttttcttctt gctctactat ccatttaaat ttatttataa accatttatc
    43501 tatacctgta atttgttcta tcatttctac tttatagcct cttcttatca tttcagctaa
    43561 atcaaatagt ctctcatcat caggtactac aactcttttc tttaattcct caatgcttct
    43621 ctcctcagat ggtgtgtgaa ctaatgaata ttttcctgtt tcaagtgatc ttatcccttt
    43681 aagaatagca gcctcaaagt tacttcctat actcataatt tcaccagttg ccatcatctt
    43741 agttcctaat tttctatttg cttttttaaa tttatcaaaa ggccattttg gtatttttgc
    43801 caccacataa tctatagttg gttcaaaaca agcataagtt tttttagtta ctgcattttc
    43861 tatctcatct aaagtataac ctaaagctat ttttgacgaa actttagcta ttggatatcc
    43921 tgtagctttt gatgctaaag ctgatgacct actaactctt ggatttatct ctataactgc
    43981 atattcaaaa ctatgtggat taagtgcaaa ttgaacgtta caaccacctt gaacttctac
    44041 tgcatttaat atatctaaag atgctttttt aagcattgca cattctttgt tacttaaagt
    44101 ttgagttgga gcaacaacta tactgtctcc tgtatgaact ccgactgggt ctatattttc
    44161 catgttacat acaactatac aatttccctt tgaatctctt attacctcat attctatttc
    44221 tttccaacct tttatactct tttcaattaa tacttgtcca acaggactta attgtagacc
    44281 atgtgaaagt atttctctta attcttcctc attatcagct atacccccac cagttccacc
    44341 tagtgtgtat gctggtctta caactactgg ataaccaatt gtaagtgcat aatcaagacc
    44401 tgcttgtaaa tctgtaacta tatcactaac tattacaggc tgatttattt ctttcattac
    44461 ttctctaaat atatctctat cttcaccttt tttgatagat tctactgaag tacctattat
    44521 ctttactcca tatttatcta gtatgccttt ttcataaagt tcaactgcta ggtttagccc
    44581 tgtttgtcca cccatacctg caagtaaact gtctggtctt tctttttcta ttattttttc
    44641 tataaattct attgttaatg gttctatata tattttatct gccacttctt tatcagtcat
    44701 tatagtggct ggattactat ttattaatac aacttcaatt ccttcctctt tcaaagactg
    44761 gcaagcttgt gttcctgaat agtcaaactc tgcagcttga cctattatta ttggtcctga
    44821 ccctaatact aaagttttct ttatactatc taatttaggc ataaattttt ctccttatct
    44881 aaatggtttt tactatctgt aaataatact aaatctatat taatgctaag aatttattaa
    44941 atatacaatc taaatcttct tttgttgtgt attctggaat gtattgaaca ctatatattg
    45001 gtaattcctt atgtctcatt ccttctatag tattgtcatt taaatttatg tgagttactt
    45061 ctgtattttc tggcatttct gaaacattat atccatgatt ttgagaagtt ataaatacct
    45121 ttccagtttc taaacattta actgggtgat tacctcctct atgtccaaat acaagctttg
    45181 aagtctttcc tcctaatgcc aatgctaaaa tttgatgtcc taaacctatt cctgtcaatg
    45241 gctttttgcc tattaatttt tttatatttt caactatatc atttatatcc tctggatttc
    45301 ctgggccatt agataataag attaagtcag gatttatatt taaaatttct tctactgtag
    45361 tcattgctgg tactatagtt atatcacagt tacaagcctt aagagactct agtacactat
    45421 ttttaacacc aaagtctata actacaactt tagctccagt accttttata ttttcaattt
    45481 cttttcttgt aacatttttt actgaatctt tgttacaatg ctcttctaat ttagactgaa
    45541 catcttgtaa tgatacattt tctaaagtta taattcctct cttagttcca ttgtttctaa
    45601 taattttagt taatgctctt gtatctattc cttcaagtcc tattacctta ttttgcttta
    45661 gatatgtgtc tatatccatt tcacatctaa aattatttgg gttatcactt ttttctctta
    45721 caataagtcc ttttacttgc accttatttg attcaacatc ttctaaattt attccatagt
    45781 ttcctataag tggataagtc atagtaacta tttgtccata ataagttgca tctgtcaata
    45841 gttctccata cccagccatt gaagtattaa atacaacttc tccaatagct tcctttagat
    45901 atccaaatgc ttttccttca aatactgttc catcttctaa aattaatttc cccttcatgt
    45961 aagctacctc ccaaatattt tatctaagca ttttctattt ctcttacaat attaatagct
    46021 gattctcttg gattttctgc ttttgttata ggtcttccaa caactaaata atgagcacct
    46081 ttttttattg catctgatgg agttacaact cttttttggt ctccaacctc tgctgattta
    46141 ggtcttattc ctgggcaaac tgttataaaa tcagttccac atgcttctac tattttatct
    46201 acttcttgcg ctgaacaaac aaccccatct attcctgatt cttttgccag tttagctctc
    46261 tttatagcta actcttctgt tgtcatatta catcctatgt cctgtaaatc ttggttgctt
    46321 aatgaagtta aaactgttac tccaactact aatggctttt ctatatttaa tctttctgct
    46381 tcttccctag caattagtgc acattgtctc attccttcca tatctgatac atgtattgtc
    46441 attaaccaaa cattatctct cactgcagct ttcactgcac ttttcatagt atttggtata
    46501 tcatggaatt taaggtctaa aaatattttt ttcccttttt ctttaagata atctattgtt
    46561 ttaccttttg tggcaacgta ttgctctagc cctaccttaa atatgtctac actatcttct
    46621 aatctgtcta ttagttcttt agctttatca aattcgtcag tatcaattgc aactatcaat
    46681 ttttctttac catttatcat ttttacatct cctttttaat aaagcctaaa taatcataaa
    46741 aaaatcctca caccaaaaga ggcataagga ttttatctat acataactct attcattagc
    46801 tatgagatat ctttcccagt ctctctggac tagtttaaaa gactttatta tatttaaatt
    46861 tttattttat actcatcaaa aacctctaga agatttacta gaggttatat actaacatta
    46921 tctataaccc tttagtctct ctggactact ttaaggaatt tagctgtaat atttttcttt
    46981 atttatataa ctttatgtat ctataggata atttaaagtt ttatcttttt tataattaaa
    47041 ccactaattg ctttttatgt caataagtct ttgaattatt cttcatcttt atcaaacaat
    47101 tttttctcaa aatatgattc tgcattatat attgcagcaa ctggattatg cccagttact
    47161 ctatctttga caactaaagt tgttatagga gcttccatat atttcatggc taaagtatcg
    47221 tgaccaacac ataatccaag aattacatta aggtcaacct tagcttcatt taaaaataaa
    47281 gcttgtccta ttggattgca cattacctca tctgaacact ttgatattgt ttgagatgct
    47341 tctagtccaa ttgtactctt aggcattgcc ccatttttac atataacaga aaatacttca
    47401 aacccatgat gttcaaatat tttttgcact gtttgcattt ctctttttag tccaatacaa
    47461 aatgctatgc caatcttttt ataaccacac tttttagcaa actctataat ctcttttagt
    47521 ctagtatatt gacaataccc ctctgcttca actaatccag cattataagc tatttttcta
    47581 ttctcttctt cttcataaag ttgctttgct ttttgttgta gttcaatttc tctagttgga
    47641 catatcttca ttaatttatc catatttcca ttagaacagc ctctaagatt acattttcca
    47701 cacttataca tatccaatat ccccttccaa aattttattc atcttgcgat tcttcttttt
    47761 ctagagttac attttcttct tgggtttctt cttctggttt cacatgctcg actacagtta
    47821 taaaatatga ttttcctatt ttattcctta taggaagttt ttcatatgaa acatctattt
    47881 tattttttat acaagctata tcaaaatgtc ttaaggctac acctaagcta accttattca
    47941 tatcttcaca gtgtttctta ggtctctttt caaataaatg aagacctcgt tcatccttat
    48001 ggaaatacca cggttgagaa ttttttacag atggagctct tcttgtcaat tctaagatat
    48061 ctttccactt tctattccta tttatcttat ttatcatatc ttttactggc tttctatcag
    48121 gtctagcata tctcgtccta aacaactctt cattttttgc tggatatcca aatgctatta
    48181 ttatataagg attttctatt ttgttttcat catcttcttc tgtttttatt ccagctagat
    48241 aatctaaatc atcttcctca tcttcttcaa aattcttatt cccaatatct acaaattcca
    48301 agatatcttc tctctttaag tttccttcca accagcaagt agctattccc aatgttgtaa
    48361 ggcgcaaaac tacaccttct gtagcaaatc ctatattctg taaatagtcc tcacctttgt
    48421 ctgatgtaac tactatataa tgaggtgcct ttactttaca ttccttcccc atcaaaaaat
    48481 gtattatatg acctctatca acaacatgtg ctttaatatt taaatcttca tttaagtatt
    48541 ctaaattttt acaacttctt tttatttctt ccatcaatgg cttttttatc tttttactgg
    48601 aaaaatttct tacagatttt ctataaaaaa tggcttcata aagctccatt tatgccctcc
    48661 taactgaaat cttctgacgt ataaactcta taaatttata taaatacttt atatttttta
    48721 tagttttatt tcctttttcc tcctgcccta ttttgaaatt cataagaata tctactctag
    48781 tttaatatag cattcaaaag gcttaaattc taccttatca atgtctatac attagcataa
    48841 tttttttatt tgattttcta tgctaattta aataacaaca ctagctatta cctttttaat
    48901 taagctttgc cagaaaaatt ttttttcatt tataaatatc tatctcatta ttatattata
    48961 agtttttttc ggtattcttg tatagctttt cactaaaaaa gtatttttat ttctacttta
    49021 atattattat caacaaaaaa catactactt aaataagttt gttatcctca taatttgttt
    49081 taattaaaat atttttttta tttctcaatc tataaagtat aaatattttg aatatgaccc
    49141 aaaatctaag taaagttata atattttata agttgttcaa tattaaaaac ataagtaatg
    49201 ttataatact ttataagtta ttcaatatta aaaatatagg aggataaata tgaatttaaa
    49261 tagaaattta gaaaaatacg ctgaattagc aataaaagtt ggagttaaca tacagccagg
    49321 tcaaattcta cttataaagt caccaataga atgtgctgat tttgcaagaa atgcacttaa
    49381 agaagcatat aaatgtggtg ctaaaaatgt ttatatagag tggtctgatg aagaaagtac
    49441 tcttattaaa tacttatatg ctccagatga agcatttcat gagttcccca aatggacagc
    49501 agaacaatat gtagatatag caaaagaagg tggggcattt ttatcagtct atgcccaaaa
    49561 tccagattta ttaaaagatg ttgaccctga aagagttgca aattttcaga aggcatctgg
    49621 aaaagcttta aaagaatggc gttcttacac tctaactgat aaatgtaaat ggagtatagt
    49681 ctctgtgcca acaaaagatt gggcaaaaaa agtgtttcca aatttatcag aagataaagc
    49741 aatagaaaaa ctgtgggatg ctatatttaa atgttcaaga gtagatggtc aggaccctat
    49801 aaaagcatgg gaagaacata atgaaaatct taaatctaaa atggactttc taaataaaaa
    49861 taactttaaa actttaaaat ataaatcctc aaaaactgat ttgactctag acttgccaaa
    49921 gggtcatgtt tggctaagtg gagcatccaa agaccctaat ggaataagtt ttaatccgaa
    49981 tattccaact gaagagattt ttggaatgcc acataagttt aaagtaaatg gaactgttta
    50041 tagtacaaaa cctcttgttt atggtggaaa cataattgac aatttcttct taacatttaa
    50101 agatggtaaa ataatagatt tctctgctga aaagggacta gatacactag aaaaacttat
    50161 tgaaacggat gaaggctctc attatttagg agaagttgct cttgttccat acaactcacc
    50221 aatatccaat acggatataa ttttttataa tactctatat gatgaaaatg cttcttgcca
    50281 ctttgcaatt ggttctgctt ataagacttg tctggaaggt ggaaacaatt taaaagatga
    50341 agaattagat gaacatggtg taaatgacag tttaacacat gtagatttta tgattggttc
    50401 tgctgatatg gatattattg gtgagactta tgatggaaag caaatacaga tttttaaaaa
    50461 tggtaattgg gctttttaac atatgatttt aactaaaaag ggctgatgga agttatacca
    50521 gacttcccag ccctttttaa tgttgatgat aaatattttt tatattaatt aatctcatta
    50581 ttatagatta attcctattg ttttaaacat aagtatctaa aacttatatt tatattatat
    50641 ataaaatatc ctatttagta aaacctttat tttaatttat atgctaaaaa tttaactttg
    50701 ctatttcaac agcagacttt gcatcctttg aataataatc agcacccatt tcttcagcgt
    50761 attcttctgt caaaactgca cctcctacaa atacctttgc atttatctca ttatctctca
    50821 gtgcctgaat tgtatctttc atactttgaa cagtcgtagt cataagtgca cttaatccta
    50881 ctaattttat atttctcttt ttagcaactt ccactacttc ttcaataggg acatctttac
    50941 ctaaatctat aacttcatat ccataatttt ctagcattat ttttactata tttttcccaa
    51001 tatcatggat atcacctttt actgtagcaa ctattatttt tcctttggat acattattgc
    51061 tacttttact taataaagtt tcctttattg tatttaatga aacttttacc gtttctgctg
    51121 attgaatcaa ttgtggtaaa aatatatctc cactatcata tctttttcct actttatcaa
    51181 gagcaggaat taaaatctca tctaatatat aattttcatc cttctctttt aaaagattca
    51241 gtgttacatt ttttgcttca tcttttaatc ctctttcaac taaaatatcc aagggcaaat
    51301 ctttatcaat ctttgtagat gaatccgatt tttttgaatt atttatattg ctatattgat
    51361 ttatatagtt tatacatcct ttatctgtgt tatttaatat tttaaaagaa ttgatagctt
    51421 ccatcatact atattcattt ggatttataa ttgctaaatc aagccctgcc cctaaagcta
    51481 atgttagaaa agaagcatta agtgctttcc tatttggaag accaaatgaa atatttgaaa
    51541 cacctaatat agtttttact cctagtgtct ttaccattgt tatagctttt atagtttcta
    51601 ttgcttcttc ttgttgtgct gaaacagtta atgataaaca gtctataaat atatcttttg
    51661 gttttatccc ataagaaact gctctattaa ctatcctttt tgctatttca aatctttctt
    51721 cagccttttt aggtatcccc ttttcatcaa gtgtaagtcc aaccacacat gaaccatact
    51781 ttttaactat tggtaatata ctttctaaag actcctcttt gccatttaca gagtttacta
    51841 ttgttctacc attataatat ctaagtcctt gctctaaggc ctctacattg gaagaatcaa
    51901 cttgtaaagg tgtatctact actgcttgaa tctctcttat taattttggc attagttttt
    51961 tttcatcaat ctctggaaga ccaacattta ctccaagtat ttgtgctcca gcatttactt
    52021 gttctaaacc taaatttata gcataatcaa cattttcatt ttttaatgct tcttgtagag
    52081 attttctacc tgtgggattc aatctttctc caacaactgt aggcgattgt acttccacaa
    52141 attttgatgg tgaacagact acacatttat caattttctc gatacatccc tttgtaacag
    52201 aatttatatt ctcagatatt tcttttataa aacttggatt cgtaccacaa catccaccta
    52261 ttatagtagc accaacttca acgaactttt taaccccttc aaaaaattct tttgcatcaa
    52321 catcatatat agcctctcca tcaactatat ttggaagacc tgcatttgct tgaaccataa
    52381 cagggactgt agctcttgat gctatttttt caactattgg taaaagttgt ttaggtccta
    52441 atgaacaatt aactcctatt gcatctacac ctagaccttc tatagtagcc accatacatt
    52501 ctggcataca tccagtaaaa cttcttccac cttcatcaaa tgtcatagta cagaaaatag
    52561 gtaaatcaga gttttctttt gctgctaata cagctacttt agcttcatat aaatccatca
    52621 tagtctctat tactattaca tcaactccag atttaacgcc ttgtagtact tgtcttttaa
    52681 atatttcata tgctttatca aaacttaaag tacccatagg ctctagcatt tctcctatag
    52741 gaccaatatc taaagctaca tatcttggtt tagtattatc tacattttct atagcttttc
    52801 tagccaccaa aacagcattg tcaatcactt cttctacagt atacttacta tctaatttta
    52861 gttcattaca tccaaaagta tttgtaagaa ttacattcga acctgcctct aagtatgctg
    52921 tatgtatttc tataagttta tctggatttt gaagaccaaa tacttctgga ttttcaccta
    52981 actttaaccc tttttgttga agcattgttc ccattgcacc atcaaatatt aatatattat
    53041 ttttcagata ttgtcttatt tccacaagag ttcgctcctt ttctatatgt acagtttcta
    53101 tagtttttac aattttcaca cgacttttct ttcttagatt tatttattct tgtattagaa
    53161 atacctataa tagccactac tgactttctc ggtatcataa ttccattaga agtaattgtt
    53221 agacctattt gtttttgagc attcaaaacg tttaatatat ctatattttt ttctattggt
    53281 aaatctccat atcctgggct atatcgcata gttataaatt tatcttgctt taaaaaaacc
    53341 ttttctaaat tattttgaac aatgtcacac acttcttcta ttgctgttgt tccacatgaa
    53401 tcaattatta gtccttttgt taaatttgaa taagagtgtc gtcttatttc tttttctata
    53461 tctatcccta tagtagctgc cataattaca cactcattac attcttttaa aagttcatat
    53521 acatctttac tctctaatgt taaatttgtt ccctctaatt ctacaacatt atctttattt
    53581 attttaatag gataaatcct agataaatat ctaggattta ttttattttt agttatagct
    53641 acacattcat caatagtata tttaaggtct ttatctatct cttgacctct atattgtaaa
    53701 tatcttaaaa cttcactttg atttatgctt atatcttctc ttccaattaa attattctcc
    53761 atatatattt aaagaaaatc ctttatctaa aacatctttt gcatattcct ttgccccaaa
    53821 taaagataag tctctgtagt ttccacattc tatagctgta gctgctggca ttttatctga
    53881 ttcaagaact ttttttaatg cttcttctaa accaatctta actgttttgg catctacatc
    53941 tccccatagt attaaataaa atcctgttct acatcccatt ggagataagt ctattacacc
    54001 atctaatgta tctcttaaat aagtagctaa aagatgctct aaaccgtgca tagcagcagt
    54061 tccaaaactt tctacatttg gctgtaaaaa tctcaagtca aatttagtta ctttatctcc
    54121 ctttactcca tcaagtacac tacactttct tacaaaagga gcttttactt ttgtatggtc
    54181 tagcttaaaa ctttctactt tttccattat ttaatcctcc attatacttt aaaatttgtt
    54241 acattaattc taaaaaaaac tcttcctatc tattattgat aagaagagta tattatttca
    54301 agtcatctca tcgttgttag caggaccacc tgataaaagg ttggtatagg atcactgagc
    54361 taactctctc ccctaactct tgataagtat cttattaaac tgacttaaat tatatattac
    54421 ttttttattt ttgtaaacac tttattgtta taatatttat atcacataat cgtactttta
    54481 ttttataact aagctgattg tattattggc agttgtactt caaacacagt ttgatattta
    54541 tcgcttgaaa cagtaatttc tccatcatgt aaatttacaa tattttttgt aatcgcaaga
    54601 cccaatccag aacctccaac atttctattt ctcgattttt caactctata aaatctatca
    54661 aatatatttg gtaaatctaa cactggaatt ggttccccat aatttatcac ttttacaacc
    54721 gccatatctt cctttttctc tgtgactata tctacataat gcccatcttt tccatatttc
    54781 acagcattag ttataagatt ttcaaatgct cttaccaatt tatttggatc agcttttaca
    54841 attaattttt cttcattaaa attaattcta ctaaccataa attcttgttt aaaataaatt
    54901 tcaaattgag atactacttg acttaataac tctactaaat ttatttctat tttgtttaat
    54961 tttattgtat tattttgcat tttagtcaat tcaaacaaat catttataag aacatttaaa
    55021 tttttcgatt tttcatatgc aatatcaaca taatacatca tctgaacttc atctttatac
    55081 ttaccttctt ctatcactcc taaataaccc attatggatg tcaaaggtgt tcttaagtca
    55141 tgtgacacat ttgttattaa atctgttttt gtctgctgag cttgtctctc ttcaatagtt
    55201 atatttctaa gttgatggac tatattattg attttatgtg ctagttcatc aatatcatcc
    55261 ttttctttat cctttaatat atctaaatca tactcaatat ctatactttt ttctaagtta
    55321 ccttcatcca ttaaatttac agcactttta atgatttcta atttttgatt ttttctatta
    55381 gtctttctta taaaatctac aatttgctta tttctcttgt acgatatcat ggaataaatt
    55441 gctaatgatg tgattgtaaa taaaaatgga taaaataaat cataccctat aagacttctg
    55501 aaatctcttg cttgatatac aagttgatta actacagaca tatcactatt gacatataat
    55561 gattttatta tatacatact tgttaaaata agcattgaag ttaaagcaca gcttattata
    55621 agatctaata tgggtcgaaa catatgattt aataaacttt tatttttcaa ttttatatcc
    55681 tactccccat acagtatgaa ttattttttc tccatctgta tatttttcaa ttttatccct
    55741 tattctgctc atatgaacca ttacagtatt attagattgg taatatttct ctttccatac
    55801 cttttcaaat atttcttctg tactatatac tctgccttta ttatttgcta gtaaatataa
    55861 tatatcaaat tctcttgatg tcaaaattat ttcatttcca gccactgtta cactatgttt
    55921 ttccttatct ataattacta ctccaacctt tatgacatct ttatcttcat tattagcacc
    55981 actaaaataa tatgctcttc taatcagcga cttcactctc acagtaagtt caagtgggtt
    56041 aaatggtttt gtcaaataat catctgcacc agtcattatc ccttgtattt tatccatgtc
    56101 ttctgatttt gcacttaaca taagtattgg tatattcaaa ctttctctaa cttttctaca
    56161 aacttctatc ccatccattt taggcatcat tatatccaat atcattaaat gaatattttc
    56221 cttttttaaa atatctagag cctcttttcc atcttcagct ttaattacat tatatccact
    56281 atttaaaaga tatatctcta taaggtctct tatttccttt tcatcatcca caacaagaac
    56341 tgtattattc atttttacac atcctaactt aatctttgta tatagcttta ttcatataaa
    56401 attatactat attttagtta gtgtaatcta taaatttatt aatttctaaa tttccaatat
    56461 aatacaaatt atttacacat ttatattatt tattttgtaa atattcttct aaagttaatc
    56521 cttttgaata aatttcctcg gcagcatcct taccaacata tctaatatgc catggttcat
    56581 atgctgtacc tgttatatct tctttacctt caggatatct tattataaat ccaaacttgt
    56641 gagcattttc tgccaaccat ttagcttctt ttgtacttcc aacaaaccat ctttctggat
    56701 ttgttaaatc aatacaaagt ccagtttggt gctcactgta accaggcttt gctacatacg
    56761 catctgcctt ttctcgcccc tgagatttta cccttcttgt ataagtatcc agttgagtat
    56821 cataagatct gtatgctgaa gaccctaaaa actgtattcc ttctgaatta gcttgcttaa
    56881 ctaaatcttc aactgccttt gcaggttctc ctgccatttg tttttcttcc tctgttgctt
    56941 cttcaacaaa aggtatgttt accttagtaa tattttcagg agtatagttt ttgctaattc
    57001 catttgtctt gtttaccaat aatatatttg atatactatt tgagtgattt ttattttgag
    57061 aatttgcctc aacatcttgt tctgaatcat gttgagcagc tactattgga tctttatctt
    57121 tcttaagtaa tctatcttta gatataaata cacttataaa tactactaaa ataatggctc
    57181 ctattaattt aaattttttc ataattaaat cttctccttt ttgttatgtt tgctataatg
    57241 ttaattttag agtataagac ttaagtattt tataagtaaa tcttaaataa atcttaaatt
    57301 taatattttt aaaggatggt gataaaatgt ctatacaaaa acttctaagt aaagctcgta
    57361 aggctataca agattttgat atgatacaag aaaatgataa aattgcggtt ggactttctg
    57421 gtggaaaaga tagtttaact ctacttcata tacttaaaag ttatcaaaga ttttctccac
    57481 aaaactttga attgatagcc ataacattaa atactggagg tgttgataat tctccactag
    57541 ataaattgtg taaagaaata aatgttcctt tttatgaatt tcaaactgac ataaaagaaa
    57601 ttgtatttga tataagacaa gaaaagaatc cttgtgcatt gtgtgctaat ttaagaagag
    57661 gtgctttgaa cgataatgct aaaaaacttg gatgtaataa agtagctctt ggtcatcata
    57721 aagatgatgc aattgagaca tttttaatgt ctatgtttta tgagggtaga gtaaattgtt
    57781 tttccccaaa gacataccta gacagacagg atttaacagt tataagacct atggtttaca
    57841 tagaagagta tatgactaag aaaatttcaa aagattctaa ctatcctatt atcacaaatc
    57901 cctgccctgc aaatggtcat actaggagag agtacataaa aaatcttatt gctaatttaa
    57961 ataaagagat gcctgatttt aaaagaaatg tatttggtgc tttaaataat tctgagaaat
    58021 tgtttatatg ggataaagag aagataaaaa attttaaata ataattaaaa tttttaaata
    58081 aaaaagcaag attgtattaa ttgtaaataa tctttatata atctacacaa tcttactttt
    58141 ttatttatta tatgttatta tctttaatct atattttaat agttcttaat ag
//
LOCUS       DAMJYR010000009        57474 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.009, whole genome shotgun sequence.
ACCESSION   DAMJYR010000009 DAMJYR010000000
VERSION     DAMJYR010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 57474)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 57474)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..57474
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.009"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            194..1270
                     /locus_tag="PC471_000559"
     CDS             194..1270
                     /locus_tag="PC471_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HDF2787700.1"
                     /translation="MKAQRLNAVLEQMKKDDISQMLVSDPTSIFYLTGVLIHPGERLL
                     ALYLNLNGNNKLFINELFPVSEDLGVEMVWFNDTQNPVEIITEHIDKNATMGVDKNWP
                     ARFLLNLIELGGGSKFVNSSYIIDTLRMCKDEEEKELMRIASKLNDKAMEQLKATVSG
                     ELTEKQLVGKLSKIYEDLGTDGFSFDPIIGFGPNGANPHGEPGNALVKPGDAIILDIG
                     CIKDNYCADMTRTVFYKEIPEKGREIFEIVLEANKRAEAIVKPGVRFCDIDAAARDYI
                     TEKGYGQYFTHRTGHSIGLEVHDKGDVSSINTDTVQPGMIFSIEPGIYLPGEFGVRIE
                     DLVLVTEDGCEILNKHDKEICVVG"
     gene            complement(1373..2215)
                     /locus_tag="PC471_000560"
     CDS             complement(1373..2215)
                     /locus_tag="PC471_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HDF2787701.1"
                     /translation="MDKIKLIVDSACDLPDDIIEKYNIEVVGLNVSFGEESYISGKEI
                     DNETFYRKMSESKVLPKTSCPSPDKFLEAYHCQEESVLVINISSGLSGTYNSALLAKD
                     MFEKEGNNKKVEVIDSLSGSIGIGQLVLKAAILIKEGKEIEEIVSIINKYKDNPPFFG
                     TLETLENAIKGGRINPIAGKIINTLNFKAIIQVADGVVTPIDKARGEGNSLKKLITLV
                     ESKIKDKEEKVLFIGHANCPEKAQKVREVMEKDVKYKDVVICEIGSVMGTYTSKGAIL
                     ITAI"
     gene            2399..2971
                     /locus_tag="PC471_000561"
     CDS             2399..2971
                     /locus_tag="PC471_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDF2787702.1"
                     /translation="MKTNNMKQAILSSATNLIAKNGVQNTSLADIAKDVNISKGTLYY
                     HYASKDDIIYDIADTHLEVITSAILNCVKNVKSKNSQIEMVNLILEKISTIESRGRVH
                     MYLICEAITSNNDLKERIRLKYIEWRTTLKSEIVESLEKYNSSENEQDAESFSFLLMS
                     IVDGLVVQSLLKTEKIPYENIASFLVNHWI"
     gene            complement(3134..3406)
                     /locus_tag="PC471_000562"
     CDS             complement(3134..3406)
                     /locus_tag="PC471_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2787703.1"
                     /translation="MLRERFVVRNHHNPHTSGCCGTGGEGCCGGDNHEHSHQHSHSHG
                     HEHEHVNGVGHDHNHDDGFSGTHEHSHEHTHTHGHEHSDGHTHEHK"
     gene            complement(3533..4405)
                     /locus_tag="PC471_000563"
     CDS             complement(3533..4405)
                     /locus_tag="PC471_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HDF2787704.1"
                     /translation="MYGESKKLSIDNNIFLQYCLIILGMILSTLGINLYLAPAKLLSG
                     GVAGICVILYKLFGINQGISSFLMNIPIFIIARKYFDNKFLLISFVNMLLFSLALGLT
                     QDIAKYFPIDDTMLQCIYGGALTGIGMGLTFKARATAGGLDIIAAIMKRKYDIPMKNT
                     FLFINFFVVCAGAFLFGAKLVMYTLITMYIISFTMDIGKDCFDRKKSILLISNKYDEI
                     SKVIMNKMGRGVTFLEAEGAYTQNKKKMIYCIISANEIGKFKDLIYDMDSEAFISVNN
                     VEEVKGGGFKDKFL"
     gene            complement(4701..7808)
                     /locus_tag="PC471_000564"
     CDS             complement(4701..7808)
                     /locus_tag="PC471_000564"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HDF2787705.1"
                     /translation="MAKFKPLVDSSVKQAEAQVFDYWKDINILEKTLEKGKDDPSFVF
                     YEGPPTANGNPGVHHVLSRTLKDSVCRYKTMSGYQVKRKAGWDTHGLPVEIQVEKELG
                     LTSKQQIEEYGIAEFNQKCRESVFSFEKQWRIMTERMAYEVDLDNPYITLDNNYIESV
                     WWILNKFNKEGYIYEGHKILPYCPRCGTGLASHEVAQGYKEVKTNTVIAKFKKKDADE
                     YFLAWTTTPWTLPSNVSLTVNADVDYLKVKKGDEVYYVSKPLADKVLGEDYEVLEEMK
                     GKDLEGLEYEQLMPFVEVDKKAFFITVGDYVTTEDGTGIVHTAPAFGEDDYNTGKRYD
                     LPVIQPVDETGKFTSTPWEGRFVMEDGLDVEIIKWLASENKLYSKEKVVHNYPHCWRC
                     QTPLVYYAKPSWYIEMTKLKDKLIENNNGVKWFPSFVGEKRFGNWLENLNDWAISRTR
                     YWGTPLPIWRCECGHTDSVGSRAELAEKAIEDVNPETVELHRPYVDDIHLKCEKCGKP
                     MTRVTEVIDCWFDSGAMPFAQHHYPFENKENFDQLFPADYISEGIDQTRGWFYSLLAV
                     STFVLGKAPYKSVLVPDLVLDKDGKKMSKSRGNTVNPMELFDQYGADALRWYLLYVSP
                     PWTPTKFDMDGLKEIQSKFIGTMKNVYNFFTLYANTDDINPTEFFVEYKDRPELDRWI
                     LSKFNNLMKDVEENLAIFELNKTLRMIQDFINDDLSNWYIRRSRRRFWATELTEDKKA
                     VYNTTYELLHGLCRAIAPFAPYMSEEMYRNLTGEVSVHLAEYPKCNEELVDTKLEEKM
                     DLAKNLVTLGRASREVERIKVRQPLQKVLVDGKFEDTISDVVDLIKEELNVKEVIFAK
                     DLDEYMNFSLKPNFKEAGPLLGSKMNLFVGALSKLNAHETANKLEKGETLTVDLDGEA
                     FEFNKDLVLIGITAKEGFNVSVENNLFVILDTKLTEELINEGYAREFISKVQQLRKSN
                     NFEVLDNIVIEYCGDDEIAKAVDHFNEYIKSETLALEINRVDDKSLEEQNLNDHMTGI
                     KVIKK"
     misc_binding    complement(7901..8133)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(8308..8835)
                     /locus_tag="PC471_000565"
     CDS             complement(8308..8835)
                     /locus_tag="PC471_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HDF2787706.1"
                     /translation="MLTPIEIENKEFKKGLRGYRDEEVDEFLDIIKEDYESLCRENTA
                     LKEKLGLYQDQVNKYENIEETLKATLITAQSAAEDTCSAANKKAKIIVEEADLKARQI
                     IEQANNRVIEIRKEYDALVKEFKIFRNKFKSLLEDEIRSVDEIFYDVDEKCVNAFEGT
                     TVYNYSNDEAATTLE"
     gene            complement(8870..9655)
                     /locus_tag="PC471_000566"
     CDS             complement(8870..9655)
                     /locus_tag="PC471_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HDF2787707.1"
                     /translation="MDKLKLTNHIKDIDLKNKMFKVIDKANSCIKNYDVKSTEFLNPY
                     EVKNAVAILNSTNEIKYSVDGGYEQAERSTVFIYPFYMEYEDIEDTLRFLQIEGNFKF
                     KNISHKDYLGSILGLGIKREKIGDIIIHDSFCQVVVSSDICDFIIVNLEKVSRNNVIV
                     KEISRENIVNSSSKYKEVSFTVSSDRLDCVISGIYNISRQDSAKYINGEKVHVNYEKI
                     TSTSKIVKNDDLISIRGKGRAKVTQIGDITKKGKIKVQARLIV"
     gene            complement(9665..9922)
                     /locus_tag="PC471_000567"
     CDS             complement(9665..9922)
                     /locus_tag="PC471_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HDF2787708.1"
                     /translation="MGTIRIALYYLLDIIAWMIVIKSLMTWFPNGRQSKIFDILENLT
                     EPIEGPIRSIMYKYTNGPIDFSPLIAIVLLMFLRQLVLVIF"
     gene            complement(9941..10399)
                     /locus_tag="PC471_000568"
     CDS             complement(9941..10399)
                     /locus_tag="PC471_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HDF2787709.1"
                     /translation="MSNGIISKFKNWIVDEEEDYIEDEYESGMDDIVQEEEMNSGFST
                     AKANKIVNLHTTSQMKVVIVEPKVYDEAATIADHLKQRRAVIVNLEGLTNSEVRKSIF
                     NFMNGAVYVLDGSIQKVSKSIFILAPNNVDIDANMKKELESKAFFPWQNK"
     gene            complement(10439..11143)
                     /locus_tag="PC471_000569"
     CDS             complement(10439..11143)
                     /locus_tag="PC471_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HDF2787710.1"
                     /translation="MGSIKDNLSEIRNKIDKAAKKVDRDSKEITLLAVTKTVDVDLVN
                     EAMREGITSVGENKPQELARKYDIIGDKLNWHLIGTLQTNKVKYIIDKVCMIHSLDRI
                     ALCEEIQKRAEQINREIDCLVQVNISKEESKHGLDREEVVDFVKEVSEKYKNIKIKGL
                     MTMAPFIEDEDEIRGVFKGLKDLSKQIKELNIPNVGMDVLSMGMSHDYEIAIEEGATI
                     VRVGTSIFGERNYNKK"
     gene            complement(11153..11788)
                     /locus_tag="PC471_000570"
     CDS             complement(11153..11788)
                     /locus_tag="PC471_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2787711.1"
                     /translation="MKKVKYTRLVILGILIYLVFNILVMIISKNVDTLVLKNEKVDAK
                     IHVKGTIEKDANDQSNYLSTYISKEDSNNFEKNQNVIIEYHEQKLDAKVYKIYENNNK
                     IMVKLKISNQIVANQDTSVEEFDIIYNQMECLKIPKTSIKTKDNKRGVYVIDEQSQSV
                     KFVILEGITYENESSVFVDYYKNDINGVTSVNLYDKIILRPNSINTNIRIK"
     gene            12169..13209
                     /locus_tag="PC471_000571"
     CDS             12169..13209
                     /locus_tag="PC471_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDF2787712.1"
                     /translation="MSEILLGTGLLLLVLCCFTIFSYKAPYGMKAMGALANAACATFL
                     VEAFNLSLLGDVLGMEFFRSVGAANGSLGGVAAAILVPLALGVSPVYAVLIGLSCLDF
                     SILSGFVAGYFVSFLIKFIEKKVPPGVDLVVVILLCAPLSRLIASNVDPLVNSTLLQI
                     GQVLIEATNISPIIMGIILGGLITVVGTSPLSSMALTSIIGLTGLPMAIGALGAFGSA
                     FMNSTLFAKLKLGSKKDIISVAIEPLTQSDIISANPIPIYSTNFFGGAFSGIIVALMG
                     LVNMTPGTATPVAGFAIMFAYNPVQQVLITAGLCALFNVIAGFLGSKIFSTFKVLTAG
                     EIRGTDVEEEIA"
     gene            complement(13672..15027)
                     /locus_tag="PC471_000572"
     CDS             complement(13672..15027)
                     /locus_tag="PC471_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009170073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PFL family protein"
                     /protein_id="HDF2787713.1"
                     /translation="MNVGNILETIKMIDEEQLDIRTITMGISLLDCIDPDGDKAREKI
                     YNKIMSSAKDLVKVGKDIEREFGIPIVNKRVSVTPISIIAGATDENDYVKFAQTLDKA
                     AEDLGIDFIGGFSALVQKGYTKGDKILIKSIPKALASTNKVCASVNVGSTRCGINMDA
                     VKEMGEIIKETAELTKDAKGFGCAKLVVFCNAVEDNPFMAGAFHGVGEADRIINVGVS
                     GPGVVKRALEKVKGEPFDVVSETVKKTAFKITRVGQLVAKEASSRLDVPFGIIDLSLA
                     PTPAIGDSVANILEEMGLEVVGTHGTTAALALLNDAVKKGGVMACSHVGGLSGAFIPV
                     SEDAGMIDAVIKGALSIDKLEAMTAICSVGLDMIAVPGNTTAGTLGAMIADEAAIGMI
                     NNKTTAVRIIPAPGCDVGDMVEFGGLLGRAPVMPINTNSSELFTQRGGRIPAPIHSFK
                     N"
     gene            complement(15053..15322)
                     /locus_tag="PC471_000573"
     CDS             complement(15053..15322)
                     /locus_tag="PC471_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010963800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HDF2787714.1"
                     /translation="MKAILTVIGKDKPGIVAGISDELYKQNINIVDISQKILQDYFTM
                     IMIVDLEKSLNSFEKTVEDLVERGKKLSVDVKIQREEIFNSMHNL"
     gene            complement(15612..17567)
                     /locus_tag="PC471_000574"
     CDS             complement(15612..17567)
                     /locus_tag="PC471_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2787715.1"
                     /translation="MKKYEIFIFMSIIVLCFISFPHKVFGQEENCLEDFERWVQENKN
                     NKEEIVYTLSCDMVIDEEFRFYIPYDSNFTIDTNKYKILIKNHGRFIIDDNELNIIGE
                     GGKEGVIHIENGGSISIGINNIIATDGTALYVEEGGDLHIKSSYESVEKIKANGKDAI
                     GIYTENDIRILNKDIEVNGEHAIGVYSKGDVEIEETSIKAYSSNNESGLLKDNNKLAQ
                     SIISESKKVYIIDDYNELIPSIAEDSGYDIVKCCHRGIGVFSDEIKVSKDDKIENIKF
                     PKYMVLETSSGNRLDIDVEWDFTDYYEKLEKGENFNITGKFKTEMLNKEKIIINDDVV
                     PILNVSVIEKKPIDNLELEFQNTKNGYVAVLFYDMPYSASKVFVEYSSDGINWISEEQ
                     EDIRDQAILFFEDFKLRCFRVKVVGGLKEGYSNIVFKPGFIMGGGDNQETPDDEREND
                     DIDGDRGGGGRDDPDRDEDDNNQDGNNGNENNDSNNNQNNNNENNNNENDNSDEKQPN
                     TPKDDVESSTDSNENKNPHDIPKDGNKENNSDKEKGFYNKNSITENDDNYDLSSKLVF
                     GTNEHSFPYRESISNKNQDTNKNKSNISDYDGIINNNYKLESKEYNLKKDIIFLFCIL
                     TPIMIGSILIVNPDTRKSIIKFIKLKK"
     gene            complement(17750..19225)
                     /gene="spoIVA"
                     /locus_tag="PC471_000575"
     CDS             complement(17750..19225)
                     /gene="spoIVA"
                     /locus_tag="PC471_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage IV sporulation protein A"
                     /protein_id="HDF2787716.1"
                     /translation="MNNNIYEDISKRTQGDIYIGVVGPVRTGKSTFIRKFMEKLVIPN
                     IDNEFKKDRTRDEIPQSGSGKTIMTVEPKFVPADGVEIKIKDTVSLKVRMVDCVGYIV
                     EGALGHEEGGKQRLVSTPWSQEAMTFEKAAEIGTKKVIKDHSTIGIVVLTDGSVTGID
                     RKSYVEPEERVIQELKNLKKPFAVVLNTLSPKSEETSMLRSELEEKYEVPVLPMNVVE
                     MEEEDIEEVMEAVLYDFPLTEIRINLPKWVEGLERNHWIKSSIITTLKQSIIDIGKIR
                     DIEGIIQGFSELEFLEDTGVDNVELGEGVINIDLQTKQDLFYNVLEEKSGFKIEGDYQ
                     LLSLITRLSKVKNEYDKIESALIDAKIKGYGVVAPSLEELSLEEPEIMKQGKQYGIKL
                     KANAPSLHIIKADISTEVSPIVGNQNQGEEMIKYLMEVFEEQPADLWESNMFGKSLHD
                     LVKEQLQSKLYTMPEEIRVKMQKTLQKIVNEGSSNIITILL"
     gene            complement(19397..20692)
                     /locus_tag="PC471_000576"
     CDS             complement(19397..20692)
                     /locus_tag="PC471_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDF2787717.1"
                     /translation="MSVEVNKPSIWKNFKVLIILFVAGAFIYALPYLKNYYYDTFAQA
                     FNLTNTQMGSLGSIYGLLAMVSYLFGGFLADRISARKLLSGSLIITGISGLVLTLYPP
                     YIVVFLIHALWGITTILTFWPALVKAVRMLASENEQGKAFGFMESGRGITNAIHLSLV
                     LILFGYISSKVSDESGLTAIIVLYSAINVIVGVLVFFKLKDNEKEVSAKFDKEAFFAV
                     IKMPHTWIITIIMFCSYSANMSFYYFTPYSTEAFGATVVFASAISILAQYCRPVASAA
                     SGLLGDKVGSSKIISYGFILMIAGLLGVIFTPTKSSMIYILLIGCVAIYVSMYAIQGL
                     HYSLLEEGNYSLSISGTAIGIVATLGYLPEVLCPLAAGKILDAFPGVTGYRYYFIVLT
                     IVAIIGLVFTFIWMNMTKERRKELIEMNNNSNENTTKVS"
     gene            complement(20876..22285)
                     /locus_tag="PC471_000577"
     CDS             complement(20876..22285)
                     /locus_tag="PC471_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDF2787718.1"
                     /translation="MKKKIGFWDLVFMNISALFGIRWIAKSTASSFGLGLGSIPVWLL
                     FAFIFFVPASLICAELAATYPKDGGLGEWVKQAYGEKWGFMVSWLNWTSKIFWYSSFL
                     TFLAINIAYMLGNPDLSNNKMFILALSLIIFWLLSLVSTRGMAFGKFFTNTGALGSTI
                     PAILLIVMAFMSVVILKKAPSASIYTIQNIIPKIDANSLVSISAIIFALSGAETTANF
                     ITEMDNAKKNFPKAILTVAVLIGGIYILGSVAITMILPPDKIAASTGILDALAKVAQD
                     LGIGSWFIRIVAFGITLSVLGALILYIAGPVKMLFGNVREGVFPKQLTVTNKYNIPSN
                     AVIVQAIIVSLLLVGTNLLPSVDNIYNVLVTMTALTALFPYVLLFTSYIRLRKTRPNE
                     ERPYSIAKSDSVCINIARMVLVVTVVGILFSTAPVMETLKDNIIYEIEMIGGGLFVII
                     TGLMLWKNYEKKISNNSGC"
     gene            complement(22337..23476)
                     /locus_tag="PC471_000578"
     CDS             complement(22337..23476)
                     /locus_tag="PC471_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HDF2787719.1"
                     /translation="MSNNMRLERLRKYMNEKSIEIALIFEPDNQFYISGFKAITYSRP
                     IVTVVTQDKIELIVPGLEELHAKEVAKVDNVYVYYEIPEMKEHGISHKHYLDIILNKY
                     PKGTTVGIEKDIVSASFSEYITDKSFAIKDVGSKIFEMRYVKDAKEIEFLKIAGYLSD
                     IGIKGSLENVRVGMSELEFDVAGDNALLKYVSENYPDTYIGFANWTCSGIDRTAQPHL
                     DSNTRILQRGDIVIHSRQVWYENYRAENERTFIIGKPTERQKEVFKIAVEAQQAGLDT
                     IKAGIPARMVDEAARAVVAKYGLELYSNHRIGHGLGLSEHEEPYLRFDNELILEEGMV
                     FSMEPGIYIPGVGGFRHSDTAIVGKNGATIITNYPRSVEELILDI"
     gene            23795..24445
                     /locus_tag="PC471_000579"
     CDS             23795..24445
                     /locus_tag="PC471_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDF2787720.1"
                     /translation="MTNKELQKKRILMYFIEAAQNIMENEGIENITLRKVADMAGYNS
                     STLYKYFKNLDHLISFASIKYFKDYNLNISRCIENVNDEYKKYIIMWKLFCKHSFDNA
                     QAFYQVFFNLSSDELSYITKQYYDMFPEDLGIHDSDISLMITGESIKERNKILLNNLV
                     DTGYIPAKDLDIINDIIISFYQNLLFLKKNTGKNSDDDELTMKMISSIEYIIKHAK"
     gene            complement(24602..25621)
                     /locus_tag="PC471_000580"
     CDS             complement(24602..25621)
                     /locus_tag="PC471_000580"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HDF2787721.1"
                     /translation="MEKVCVLGTGSWGSALALGLAKKGNDVSMWTRKEEQAKKINRTK
                     ENTDYLPGVLFPNNITISTDIEKTIKDCKVIVLAVPSQAVRSTCQKIKPFIKEGQVIV
                     NVAKGLEKGTGLRLSQVCEEELPQNPYVILSGPSHAEEVARDIPTTVVVASKDLKIAQ
                     MIQDLFMSPKLRVYTNPDIVGVELGGALKNIIAFGAGICDGLGYGDNAKAALMTRGIS
                     EMSRLGIAMGANMSTFAGLSGIGDLIVTCTSMHSRNRRAGILIGKGMSLEDTLKEVKM
                     VVEGITATEVAHDVAEKLDIDMPITNAIYSVIKKGSNPKEVGIELMMRSKKHEMEEVV
                     LGDDI"
     gene            complement(25652..26266)
                     /gene="plsY"
                     /locus_tag="PC471_000581"
     CDS             complement(25652..26266)
                     /gene="plsY"
                     /locus_tag="PC471_000581"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HDF2787722.1"
                     /translation="MEIFSYIIIAVVAYLLGNISTSYIVAKRIAGVDIRTQGSGNAGS
                     TNVLRTLGKRAGAMTFLGDVLKGVMAVLISEFAARLVGIDTLLAGYLAVICVVAGHNW
                     PAVLGFRGGKGVATSLGAMLAVNPVITLMCLAVFILVVAITKYVSLGSVVGIGCSPIF
                     MIMVKNKAGLIVALFLTASVIYNHRANIKRLLNGTERKIGQKKE"
     gene            complement(26301..27620)
                     /gene="der"
                     /locus_tag="PC471_000582"
     CDS             complement(26301..27620)
                     /gene="der"
                     /locus_tag="PC471_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012063975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HDF2787723.1"
                     /translation="MSRPVVAVVGRPNVGKSTIFNKFAGKRISIVENTPGVTRDRIFA
                     EVEWLDKYFTLVDTGGIEPDSEDIILSQMRNQAMLAMDMSHVILFIVDGKAGITAADK
                     EIAQLLRKTKKPVILVVNKIDSQSQFDNIYDFYELGFGTPFAVSGANSMGFGDLLDEI
                     VENFPAGLDTEYEEDIIRVAITGKPNAGKSSILNKILGEERVIVSPIAGTTRDAIDTY
                     FEKNGQKFLLIDTAGLRRKSKIYETIEKYSVIRAMSAVDRADVVLIVIDALEGVTEQD
                     TKVAGIAHDEGKGCIFVINKWDLIEKDNKTMSNYTKDIKEKFPFMMYAPIVFVSAKTN
                     QRMNKILDTVEYVSNEHSKRISTSALNEVIGEAVMLNQPPSDKGRRLKIYYGTQTDIR
                     PPKITLFINDKDLTHFSYQRYLENKIRENFGFEGTSIKFEYRQKNKK"
     gene            complement(27654..28982)
                     /locus_tag="PC471_000583"
     CDS             complement(27654..28982)
                     /locus_tag="PC471_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF512 domain-containing protein"
                     /protein_id="HDF2787724.1"
                     /translation="MEVNVKKVNNIISKVYKDSIAEEMGIEVGDLLISVNEQPIHDII
                     EYRFLLSDEYLDVEIQKKDGEVYIYEIEKDYDEDLGVEFTNPIIDQAKSCRNKCMFCF
                     IDQLPEGMRETLYFKDDDSRLSFLQGNFVTLTNMSEEDINNIIKYRISPINISVHTTN
                     PELRQKMISNKFAGKLYGIMKRLADAHIEMNCQIVLCPGINDGKELDRTIKELAQLYP
                     YVNSVAIVPVGITKHRENLVELNIFNDKSASKTIEQIHQIQQKYLEKLGTRFAFLSDE
                     FYILSNSELPGYEEYEGFLQFEDGVGMIRKLKTEIEEYLNILPEKILKREKKVSIATG
                     HSAYEFIQSMADAMMDKFKNLQVNVYEIKNKFFGETITVSGLLTAKDLKEQLEDKELG
                     EALYITRSMLKADEEIFLDNIELNQLEELMRIKIIPCLNEGKDFVDKILK"
     gene            complement(29220..29420)
                     /locus_tag="PC471_000584"
     CDS             complement(29220..29420)
                     /locus_tag="PC471_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2787725.1"
                     /translation="MGMDIGLNEYTLAIVTLDKNMKNGGGCPIFYANDEKDLQNKALL
                     MSKCVGGMVHDMTNGTLIIVKH"
     gene            complement(29422..30354)
                     /locus_tag="PC471_000585"
     CDS             complement(29422..30354)
                     /locus_tag="PC471_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YIEGIA family protein"
                     /protein_id="HDF2787726.1"
                     /translation="MDRVFFVAVIIGITSRIIMLNLDQKQYPTQPNVLLSQLILAFVA
                     SSLGALLVPALINRSYTSITFLSLAAEQFRQVRANRRNTLQNLEEVQLVKRGAAFIEE
                     IARTYEVRNYMCIVTSFLTIGLYYVILAETKISEGMSLVISSICGLVLAFILKKLLTR
                     KSIGDIADVMPAEISFVDESILKVGDLKGITNIGLKSDREVYINRGVGIQIIPKNKDY
                     ANAGILFDSGQRQAIVYNIYSRLGLYGDYGEPSFVPLPRRNKEDESIMIAYLPVDKNV
                     EKVMEAVKSCPILSSSKGKNLSLKNYKIGKKGSA"
     gene            complement(30399..31331)
                     /locus_tag="PC471_000586"
     CDS             complement(30399..31331)
                     /locus_tag="PC471_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YIEGIA family protein"
                     /protein_id="HDF2787727.1"
                     /translation="MEESLLNANLFRHSFIVALVVGILCRGLVLRVTDKQYPSRPQDY
                     LEQIIISGLSASLGAIALPALIDKEFAALTFFAVAIQQFQGLAEQERITLKNIDNEEL
                     VPKGDAYVEEIASTYESRSYISLFSALVSSIVYIVFARKYGLSFFYCTILAIVSGAIV
                     GLIFRRFLRRNSIADIADIVPAKISFEGPILMVNGVIITNIGLESTREKYRNECLAIE
                     VIPRDLGAFGIVNDIGQRQAIIHNLFIHMGIDRDVDEVDIVAISKTNLEKSTVVIPYM
                     PILKDIDVMIDVVKSTPIIETSKGKQSDFSRKTL"
     gene            complement(31386..32675)
                     /locus_tag="PC471_000587"
     CDS             complement(31386..32675)
                     /locus_tag="PC471_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDF2787728.1"
                     /translation="MEDLNSIYFFIVMLSIAIVILAVRYTITLRKYLKEFIYVSKKVS
                     NNEYHARLNISAKGELGELARNFNHMIKNMDKTLEEVQYKHLQLRSILKSISHGILAI
                     DVNGNILLINDEAKEMIKSEPNQLVEGHNINVVIKEEEILKQILQFIGSKDNETANIT
                     TKDDIVYKIKVDPVYLQNTNNVIIGSIVNIEDITKKVKLENMRSDFVANVSHELKTPL
                     TSISGFVETLKSNENIDVNTRNRFLEIIESESDRLKRLIDDILLLSFVENKDTLLMEN
                     VVVYDVFKEVYEITQHSASEKNIEVLYKCDDKSISILSNKDYIKQVFLNLVDNAIKYT
                     QDNGIVQVVVLKDEEKIVIKVIDNGVGIPKEDVERIFERFYRVDKARSRDVGGTGLGL
                     AITKHIVKSLNGCIKVKSELGIGSEFIVTILQKNFLK"
     gene            complement(32704..33396)
                     /locus_tag="PC471_000588"
     CDS             complement(32704..33396)
                     /locus_tag="PC471_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2787729.1"
                     /translation="MNTKVLVIDDEMHIVELLKFNLEVSNYEVSYSYDGFDGFIKAKE
                     IKPDLILLDWMLPNISGIEVLRKIRSDKDLKNIPVIMLTAKNMENDKVEGLEIGADDY
                     ITKPFSIKELLARISSVLRRYNLTSLGEENNILTTGNLKLDLSKHEVTKGSEKIELTL
                     KEFELLKLLIQNKGKVLSRNYLLDKIWGYEYYGETRTVDVHIRYLRKKIEDEDKSEKY
                     IETIRGVGYKID"
     gene            complement(33411..34172)
                     /gene="pgeF"
                     /locus_tag="PC471_000589"
     CDS             complement(33411..34172)
                     /gene="pgeF"
                     /locus_tag="PC471_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HDF2787730.1"
                     /translation="MMKDYINIKNVQDIKEYVNLSGNCEELDEKLNSVNLVITCKNID
                     AKNREDMLKVCKECDLTFENLTSNSQIHSDIVNIVNKDTIGKRRDGDALITNLEKVPL
                     LLFTADCVPISIIDAKNKAIGLAHAGWRGTFSNIGHKTIKLMSECYKTVPEDLVCIIG
                     PSIGPCCYEVSEDLVEKFNTILTNNDEKFYIIKEGSYYLDLWKINEYMLRCSGVKKEN
                     IINLNLCTSCRADKFHSYRKHNKASERIGTVLQIK"
     gene            complement(34169..34630)
                     /gene="nrdR"
                     /locus_tag="PC471_000590"
     CDS             complement(34169..34630)
                     /gene="nrdR"
                     /locus_tag="PC471_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HDF2787731.1"
                     /translation="MQCPYCNYKESKVIDSRHTDLKSIRRRRECESCKKRFTTYEKIE
                     TTPLMVIKKDNSREYFDREKIKYGLLKACEKRPVSIDEIESIVVHIENEINKCFIEEI
                     ETKKIGEMVMDKLKELDEVAYVRFASVYRQFKDINTFVNELKSILIEKGDK"
     gene            complement(34652..34915)
                     /locus_tag="PC471_000591"
     CDS             complement(34652..34915)
                     /locus_tag="PC471_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlmC/YmxH family sporulation protein"
                     /protein_id="HDF2787732.1"
                     /translation="MIRVSDIMEKEIINVKNGKRMGFIIDIDMDIHEGKVVSFTIFGD
                     GSRSFFSRGGDEEVIFWNDILKIGCDTIIVNIGSELSLDEFEI"
     gene            complement(35019..35792)
                     /gene="sigG"
                     /locus_tag="PC471_000592"
     CDS             complement(35019..35792)
                     /gene="sigG"
                     /locus_tag="PC471_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011986856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigG"
                     /protein_id="HDF2787733.1"
                     /translation="MQVNKVEICGVNTSELPVLKNKQMKELLLQIKNGDEEARQQFVR
                     GNLRLVLSVIKKFNNRGENIDDLFQIGCIGLIKAIDNFDLSQNVRFSTYAVPMIIGEI
                     RRYLRDNNPIRVSRSLKDIAYKALQVRERLIRTNSKEPTVSEIAKELELEVESVVMAL
                     DAIQDPISLFDPVYQDNGDAIFVMDQVQDKKDTDENWLQEISLKEAIKKLNSREKLVL
                     DLRFYKGRTQIEVADEIGISQAQVSRIEKNALKNMRKYV"
     gene            complement(35884..36606)
                     /gene="sigE"
                     /locus_tag="PC471_000593"
     CDS             complement(35884..36606)
                     /gene="sigE"
                     /locus_tag="PC471_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigE"
                     /protein_id="HDF2787734.1"
                     /translation="MLRLKERIISFITMLGIKLKIIKPKGIYYMGGANILPPPLKPEE
                     EMELLQKLETDESVKSILIERNLRLVVYISRKFENTGIDVEDLISIGTIGLIKAVNTF
                     KLNKNIKLATYASRCIENEILMYLRKNNKKKTEVSFDEPLNIDLDGNELLLSDVLGTE
                     NDEIYKIIEEEIDRDLLVMALDRLSDREKQIMELRFGLIDKGIEKTQKEVAGMLGISQ
                     SYISRLEKKIISRLQKEMKKFV"
     gene            complement(36631..37503)
                     /locus_tag="PC471_000594"
     CDS             complement(36631..37503)
                     /locus_tag="PC471_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E processing peptidase SpoIIGA"
                     /protein_id="HDF2787735.1"
                     /translation="MFKGFKGGYMVYIEYYVIENLVINYIIISCTSILTKRYNPKKNK
                     LLGASLGGAYSVAYLYPSMNILFTVPFKIIIMSIITLISFRSKSKKDYVHVALVFYLV
                     NIFISGSTYFIIYFTGISHLKISFLIACTYVSCELLKYIYKDLKMLKYIKELTKTINI
                     NLLEKSFCCKALVDSGNLLKDPISQSDVIIVKSSVLEGFIPEKLLNINYEDIDMKKAQ
                     EIIGSLDDKTSGRVRIIPYKHAGSNKTSIIIGLKADYIEVDEQKIGNIILGISNFNDR
                     EYGAIMNPNILPGF"
     gene            complement(37793..39067)
                     /locus_tag="PC471_000595"
     CDS             complement(37793..39067)
                     /locus_tag="PC471_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HDF2787736.1"
                     /translation="MIKLKKVLSLVMVGILSVSILAGCSKKEDSKGKEGKEKLVVWSA
                     PLTDHDADAWKPIFEKFEKENNCEIEFQIVPWDNYAEKYATAISAGEGPDIGYMYAEM
                     FPQFIEMGAVEDLTPYLEKSGTSDNYLYLDDAKMMGGIYGLPIEAANPGVLYYNKDIL
                     EKLGEKPPKTWDDFKRICEKATKDTDGDGKIDQWGLAQGWGSKVFGSLNWNWYPYLWQ
                     AGGDIYNDDLKSVKFNNAEGLKAANFINDMKKYIPEDALSKDSNEMIESVFGPGKAAF
                     TIMLSSAATSTFDKSFPDLNWGYVTSLEDKTVGSFASVDDLTLMSACKNKELGFKLLE
                     YMLSKESMEAFHKEIPRAPVAKGEKYQGDPRFEEMVTRDKHMYRPLTVGPHGVEIYEY
                     LWKQLQMMIAGDITPEQALNESAKYANGLLAQ"
     gene            complement(39351..40508)
                     /gene="ftsZ"
                     /locus_tag="PC471_000596"
     CDS             complement(39351..40508)
                     /gene="ftsZ"
                     /locus_tag="PC471_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HDF2787737.1"
                     /translation="MLNFDVELEECAQIKVIGVGGGGNNAVNRMVEAQLKGVEFISVN
                     TDKQALYTSKAEYKVQIGEKLTRGLGAGANPEVGKRAAEESKDEIVKLLQGADMVFVT
                     AGMGGGTGTGAAPVVAGLAKEMGILTVGVVTKPFAFEGKIRMKNAEGGIAELKSKVDT
                     LITIPNDRLLQIVQKNTSMLDAFAVADDVLKQGIQSISDLIAVEGLINLDFADVTTIM
                     KDKGLAHMGIGSASGETRAIDAARQAIQSPLLETSIQGAKGVLLNVTGGPNLGLFEVN
                     EASTLVMESCDPEANVIFGASIKEDLGDEIMITVIATGFEGLQNGALDLDTKPKSSIR
                     SSLNTTVKQAVKEIEEEVIAEEKIEPPKKASIIEEDDDESMEIPTFLRRRR"
     gene            complement(40698..41051)
                     /locus_tag="PC471_000597"
     CDS             complement(40698..41051)
                     /locus_tag="PC471_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small basic family protein"
                     /protein_id="HDF2787738.1"
                     /translation="MLWVLGGLAFGVVVGYYIPFTYSMDYSIYMSVAILAIMDSIFGA
                     IKSNFEDNYDNVIFITGFIGNAILAILLTYIGEKLGVSLYYVAVLVFGMRLFNNLSII
                     RRYIISNIMNKKRKN"
     gene            complement(41150..41824)
                     /locus_tag="PC471_000598"
     CDS             complement(41150..41824)
                     /locus_tag="PC471_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF881 domain-containing protein"
                     /protein_id="HDF2787739.1"
                     /translation="MKKKIVILGAFILGVFISTYIKSLDPNRVYITLGQTESIESEVE
                     NTKKEIKNLKETLKNNKKTLNEYKEAKKNGNESIQTLMQKELEEVKELSGYSTVTGPG
                     ITITMRDSERELKDGQNPNDLIIHDIDILRVLNDLKKAGARAISINGERVLATSKIKC
                     SGATITVNDTTYGQPFIIKAIGDIDLLSAAINSPESYAKSLKDIYGINIKVQENNGNK
                     VDLFAK"
     gene            complement(41829..42527)
                     /locus_tag="PC471_000599"
     CDS             complement(41829..42527)
                     /locus_tag="PC471_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF881 domain-containing protein"
                     /protein_id="HDF2787740.1"
                     /translation="MKKRFINKSIIIGSIVLGVLVSLQLKIINIENRGMTTFKRGEQL
                     TEELKSLKKEKENLQSEVNSVKKDIETYRGKNNEGEQDILKSEIEKYETLAGYTDVEG
                     TGIKIEINSTKDLGNSDKNDSILYNYDLLLSMINKLNSAQASAISINDQRIVSNTYMY
                     LKEDKLYMNDTVIKEPIIIKAIGDSDTLASALNIKYGIVWEIETYYNAKVKVTKEDNI
                     EINGHSDINKAEGK"
     gene            complement(42550..43290)
                     /locus_tag="PC471_000600"
     CDS             complement(42550..43290)
                     /locus_tag="PC471_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HDF2787741.1"
                     /translation="MKKRRKLNTNNVMIVLVFILMLSFCICIIVKSDLFDVKKIDVIG
                     NKRVTKSNIMKELNVNLNENIFAYNFKDMKNKLIKNPYIENVEIKRKLPNKIIISLKE
                     KEIFAVLKDEDNYCYIDKKGNLLEELRGSNESKKDLIVDVDYSIDDNKSIKFKNYKTK
                     ENVFKTLNYLKEEGIYRKINYVNLKKESNIEMLTRSNIKILLSNDDNLDYNISRVSKI
                     LIDLQNKNTNGGTINLNYGKLAVYSPEG"
     gene            complement(43560..44786)
                     /gene="murG"
                     /locus_tag="PC471_000601"
     CDS             complement(43560..44786)
                     /gene="murG"
                     /locus_tag="PC471_000601"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HDF2787742.1"
                     /translation="MKVLLSGGGTGGHVYPAIAIANKIRDEHPDAEIIFVGTEKGIES
                     EIVPKYGFELKTVTVQGFKRKIDFDNVKRVFKLFKGLEQSRKIVKKFKPDIVIGTGGY
                     VSGPVLFNASMGKIPAIIHEQNSFPGVTNKILSKTVTKVLTSFEDSHKRFPEAAEDKL
                     VFTGNPVRKEILLSRKNIARKNLSISDEKRMVLCYGGSGGSRKINDAMRLVIKNMVNE
                     DIAFIFATGKSYYDEFMGSISDINLKPYQKVVPYLEDMANALAASDLVIGSAGAISLA
                     EITALGKPSIIIPKAYTAENHQEYNAKSIEKQGAGIAILEKNLTPESLNTAVFKLLGD
                     RELLVDMANASKTIGKPEAIDLIYDEIMKVYNSTQKSTSKKTKKEKVIKEVKEIKKET
                     TPSIEGQAKVIGIKKR"
     gene            complement(44804..45934)
                     /gene="spoVE"
                     /locus_tag="PC471_000602"
     CDS             complement(44804..45934)
                     /gene="spoVE"
                     /locus_tag="PC471_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein E"
                     /protein_id="HDF2787743.1"
                     /translation="MPKENLKKQIDIRVKTEFDGVVFYTTMLLVFVGIVMVFSASFIQ
                     SSFKHNDAYYFLKKNVIYAVLGFIVMIITSRIDYSFWKKNATAIGAIAVVLLLLVLTP
                     LGIEANGAKRWLGIGALTFQPAEIAKFATIILTAKLIEKNYDKIKSLTKGVVPLLVVP
                     GIFFALIILQPNLSTAGTVILVTFVMIFVAGMDMKIVFAMIGSGAALFAALVIAEPYR
                     LSRVTSFLDPFQDPLGKGYQVIQGLYALGSGGLFGLGLGKSKQKYFYIPEPQNDFIFA
                     IIGEELGLIGCIIVIMLFVVLVYRCVRIALKTSNVFACMVVIGIGAQIGIQAALNIAV
                     ATSSMPATGVALPFISYGGTSLTIFMGAVGIVLNISKHVKIN"
     gene            complement(45986..47341)
                     /locus_tag="PC471_000603"
     CDS             complement(45986..47341)
                     /locus_tag="PC471_000603"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HDF2787744.1"
                     /translation="MDLKGKKVLLVGLAKTGISTIKHLDKLGASIIVNDIKDENKLRN
                     ILDELKSINDIKYILGHHPEDVDDIDMVVVSPGVPLDLPFILKLKNSGKYIIGEVELA
                     FKLSDNPIFIGITGTNGKTTTTSLVGEIFSRAKRDTYVVGNIGNPVIDTIETSSEESV
                     LVTELSSFQLESIDEFRPKVSAILNITEDHLNRHHTMEKYIEAKANIFMNQTVEDFCV
                     LNYDDEIVKSLADKCNAKVIYFSRTKKVNGGVYLENNDIIIDIDDKIKFLNKDDVSLP
                     GGHNLENCMAAIAIAYVCKIDLEVIKDVLMTFKGVEHRQEFVRNLDNVIYVNDSKGTN
                     PDSTIKAIQSYDRPIILIAGGMDKGSNFDELLETAKSYVKSLVLLGETASNIENCAKN
                     KGFNDIHIVKDMEEAVKTSYEISKSGDIVLLSPACASWDMYESFEVRGKDFKDNVNNL
                     K"
     gene            complement(47360..48328)
                     /locus_tag="PC471_000604"
     CDS             complement(47360..48328)
                     /locus_tag="PC471_000604"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HDF2787745.1"
                     /translation="MMLGITELTYTALIAFLIVIIIGPIFIPMLRKFKFGQTVRDDGP
                     QTHLAKNGTPTMGGIIMIVAILITGLTRVKVSHDMAVGLICIAGFGFIGFLDDFIKIK
                     LKRSLGLKAYQKIILQVALSFYVAFYQYTSSSSASQLMIPFTDFVINVGILYIPIMMF
                     IIVAIVNAVNLTDGLDGLASGVTLIVSVFFMLFASSIAGNTEVAVLAAATVGACLGFL
                     GFNSYPARVFMGDTGSMALGGAVVAFSVLTNSVLIIPIIGGIYFAEALSVLIQVGYFK
                     ATRKRFFKMAPIHHHFEQCGWPETRVVFIFWIITVVLAWISIIAVF"
     gene            complement(48369..49742)
                     /locus_tag="PC471_000605"
     CDS             complement(48369..49742)
                     /locus_tag="PC471_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HDF2787746.1"
                     /translation="MECLTIKELVLATNGKLIYGDYNDCVSDIVIDSREANAQNAFVA
                     IVGENLDGHTFMKSAYDSGCKTFIKNESNGIKLESSDINLIEVKDTSLALGDISKYYK
                     EKFDIPFIGVTGSVGKTTTRDMIYSAISAKLNILKNEKNLNNHFGVPLTLFNLNKEHE
                     CAVIEMGMSGFNEIKYLVDIVNPKIAVISNIGLSHVEKLGSQEGILKAKMEITSNFDE
                     TNTLIVNGDDKFLSTLKEKEHVYKLKTFGFNKNNDVYCESYTMEEDSLTFICVIDGKK
                     EEIFIPTIGEHNIYNAMSAILVGLCLNISLDYIKKGLKNFKGTKMRLDIIKNEKLTII
                     NDSYNASPDSMDAALKILGRYKGRRVAILGDILEMGEISEYGHRLVGKSSMNNTDIII
                     TIGENSVFIGEEAKQLGFNSANIYHFENREDVFNKLNELVKTGDTILVKGSRGMRLEK
                     IVEYLNK"
     gene            complement(49909..51888)
                     /locus_tag="PC471_000606"
     CDS             complement(49909..51888)
                     /locus_tag="PC471_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein D"
                     /protein_id="HDF2787747.1"
                     /translation="MRKVKRISKKRLVLVLILACALFFCLVIRTGYLQLMKGNWLSTK
                     ALEQQTRDIPIEPKRGTIYDRNMKELAVSVTKYTVWCKPVEVEDKKEAAEKVAEILDE
                     DYKDIYALISKKNMALVKVKRWIDDDKASQIRDAKLSGIWVAEDNQRYYPYGNFAPYV
                     LGHTSSDATGISGVEMQYDKKLKGKPGKLIVSTDASGREIPQGMEKYYEPVQGNGLVL
                     SIDEVIQHYTEKAVQKAYELNNAKKVTAIAMNPKTGDILALASKPDYDPNDSRTPIYP
                     YYQEELEKYNDKDKIKGYYQMWRNPAVSDTYEPGSTFKLITSSSALEEGVIKDGEKFT
                     CTGSVTVGGRKIKCWRHYRPHGTQEFKQAVQNSCNPVFVELGSRLGVGKMYDYIESFG
                     LMDKTGIDLPGEAKGILYNEKNVGPVELATISFGQSISVTPIQLITAISSIANGGDLM
                     QPRVVKSYTDNKGNITETVKPKKVRSVISKETSKKMLEIAESVVTEGGGKIAYIPGYR
                     LGGKTGTAQKVIDGKYAPGKYICSFVGIAPCDDPQIVVLAIVDEPTGVSAFGSTTAGP
                     IVKEIMNDSLKYLGVKPVYKEEEKAEYEKKQVKVPDVRNLKIGDAVKALEDAKLKPDL
                     DADIELPEDTKVKDIFPKPGVKVNEDSSITLYFEN"
     gene            complement(51900..52247)
                     /locus_tag="PC471_000607"
     CDS             complement(51900..52247)
                     /locus_tag="PC471_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2787748.1"
                     /translation="MKKNYKIHNLNEYKNKKKNNYKKRNKKKIGAKCILLIATFSVII
                     LNLCGYSKISEMKYDIYYLQKDLRKKEIVLEELKAKETEKTSIKEIELKAKELLNMDY
                     PKDGQIKYIDVDN"
     gene            complement(52728..53663)
                     /gene="rsmH"
                     /locus_tag="PC471_000608"
     CDS             complement(52728..53663)
                     /gene="rsmH"
                     /locus_tag="PC471_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HDF2787749.1"
                     /translation="MEFHHVSVLLNECIENLNIKPDGVYVDCTMGGAGHSKEIVKKLS
                     DKGLFIGFDQDKNAISTAKERLSEYESRVKFVHSNFENIKEELEKIGVYKIDGVLADL
                     GVSSHQLDEADRGFSYMQDAPLDMRMDVRCEFSAYDVVNTYTEDELTKIIKDYGEDNW
                     AKRIAKFIVEERANKPIETTGELVDVIKKAIPKKARIDGPHPAKRTFQAIRIEVNNEL
                     GVITKMINDASSIMNEGGRICIITFHSLEDRIVKNAFKHLASDCICPQHLPICQCDKE
                     SEVKIITRKPILPSEEEIEVNPRSRSAKLRVAEKI"
     gene            complement(53693..54439)
                     /locus_tag="PC471_000609"
     CDS             complement(53693..54439)
                     /locus_tag="PC471_000609"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HDF2787750.1"
                     /translation="MDRVAFTLFGIDIMWYGILMACGMILGTLIAIKEAKRVGIKEDD
                     VLNIAIIAIPVGLICARIYYVVFNWSYYAQNMSQIFNFRGGGLAIHGGLIGGILAGYI
                     YTKIKNINFLKMADTVILGMPLAQAIGRWGNFINGEAHGGATNLPWGIMVDGVKVHPT
                     FLYESIWDFGIFIVLLLFRKNKKYEGQVIVTYITLYSIGRFFIEGLRTDSLMLGPLRM
                     AQVISLIGVIGGIIAHVYLSKKNKNNISEE"
     gene            complement(54833..56119)
                     /locus_tag="PC471_000610"
     CDS             complement(54833..56119)
                     /locus_tag="PC471_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HDF2787751.1"
                     /translation="MEKIVNDILKEEVYYEKLQNGLDVYFMPKRGFMKKYAILATNYG
                     SNDLEFVPIGEDKKIRVNEGIAHFLEHKMFEQPDGGDAFDKFSKLGVNANAFTNFTMT
                     AYLFSATENFYESLEHLIDYVQTPYFTDENVEKEKGIIAQEIKMYNDDPDWNVYFNCL
                     KAMYVNYPARIDIAGTVDSIYKITKEELYKCYNTFYNPGNMALFVVGDLDVEKVIDVT
                     KKSNNYKVDRLSKSIERFYPEEPESVKEKEVIEKFPISMPMFNIGFKDSNVGLKGKEL
                     LRKEIVTDILVGMLFKKGSKLYEDLYMQGLINENFGAGFSSQVDYAFSIIAGDSKEPK
                     KVKEIILDYIEKSKKEGLSKEEFERTKKKKIGSFIKCFDSINFIGNSFISYVFKDINL
                     LDYLDIIKDITFEEVEERLKEHFKEEYCVISIVEPK"
     gene            complement(56134..57399)
                     /locus_tag="PC471_000611"
     CDS             complement(56134..57399)
                     /locus_tag="PC471_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HDF2787752.1"
                     /translation="MVNTKKIINLGNNINLTLIKTEKFKSNLISLYVQRLLDEKETTK
                     NALIPSIIASGSAKYPSARAISNKLDDLYGSSMGADAVKRGERQVLSFKVINISEKYL
                     DESIFEEVVEFFNEVINNTLVVDGGFKEEYLNIEKENLREKIQSIINDKKEYAQDKCI
                     EAMCKGERYSVSEFGYEDEIDSITSRELYEHYKNILKTSPIDIVVEGNFDEDKVVDII
                     SKNLKFEREEIINIPRADFIKNVDEVKVIDEQMEITQGKLVMGYRANVDYADIDKYYA
                     LVVGSNVLGGGPHSKLFVNVREKESLCYYIFSSIEKYKSIMFISSGIETKDYDKAVEL
                     IKKQVESVKAGDISDEELENSKLALVNSMKSITDNIGGMSDFVFSQSMAKTNSEVQDI
                     ISSIEKVTKKDIVEAIKNIELDTVYFLRN"
BASE COUNT        17910 a        10514 c         6100 g        22950 t
ORIGIN      
        1 atttattact tttatagaat tttcacaata aaaagtcatt ttacttccgt atttaatgca
       61 aaagtagaaa ttaaacttaa tataaaaaat atataacaaa ttttttttat atttctagaa
      121 gaaaagtata ctcttttttg tttaataatg ctaaaattga tttgtattga attttgatta
      181 ggaggaaatt attatgaaag ctcaaagatt aaatgctgta cttgaacaaa tgaaaaaaga
      241 tgatatttct caaatgttag taagtgaccc tacttctata ttttatctta caggagtact
      301 aatccatcca ggcgaaagac tattagcatt atacttaaat ttaaatggca ataataaatt
      361 atttataaat gaattattcc cagtttcaga agatttaggt gttgaaatgg tttggtttaa
      421 tgatactcaa aatccagtag aaataataac tgaacatata gacaaaaatg ctactatggg
      481 tgttgataaa aactggccag ctagattctt attaaactta atagaattag gtggaggaag
      541 taagtttgta aattcatctt atataataga tactttaaga atgtgtaaag atgaagaaga
      601 aaaagagtta atgagaatag cttcaaaatt aaatgacaag gctatggaac aacttaaagc
      661 tactgtttct ggtgaactta cagaaaaaca acttgttggt aaattatcta aaatatatga
      721 agacctaggt actgatggat tttcatttga ccctattata ggttttggac ctaatggagc
      781 taatccacat ggtgaacctg gaaatgcact tgtgaaacca ggagatgcaa taatcttaga
      841 tataggttgt ataaaagata actattgtgc tgatatgaca agaactgtat tctataaaga
      901 aatacctgaa aaaggtagag aaatatttga aatagttctt gaagctaata agagagctga
      961 agctattgta aaaccaggtg ttagattctg tgatatagat gctgctgctc gtgactacat
     1021 aactgaaaag ggatatggac agtacttcac tcatagaact ggtcactcta taggacttga
     1081 agttcatgat aaaggagatg tatcttctat aaatacagat actgtacaac caggaatgat
     1141 attctctata gagccaggta tatatttacc aggtgagttc ggagttagaa tagaagattt
     1201 agtacttgtt actgaagatg gttgtgagat attaaacaaa catgacaaag aaatatgtgt
     1261 agttggttaa ttctaaaatc aatttgataa aattaaaaag ccatataaaa atgttttaaa
     1321 catttttata tggctttaat ttctcaatct atgatttatt tcaatccatt atttatattg
     1381 cagttattaa gattgcaccc tttgaagtat atgtccccat cactgaacca atttcacaga
     1441 ttacaacatc tttgtatttt acatcctttt ccataacttc ccttaccttt tgagcctttt
     1501 ctgggcaatt agcatgtcct ataaataaaa ccttttcttc tttatcttta atttttgact
     1561 ctactaaagt aatcagcttt ttcaaactgt ttccttcacc tcttgcctta tctataggag
     1621 ttacaacgcc atctgcaact tgtattatcg ccttaaaatt taaggtattt ataatttttc
     1681 ctgctattgg atttattcta ccacccttaa tagcattttc taaagtttca agtgttccaa
     1741 aaaatggagg attatcttta tatttattta ttattgaaac aatctcttca atttcttttc
     1801 cttcttttat taatattgct gcttttaaca ctaattgtcc tattccaatt gacccactaa
     1861 gtgaatctat aacctcaact ttcttgttat ttccttcctt ttcaaacata tcttttgcta
     1921 aaagtgcact attataagtc ccagataatc ctgaacttat attaatcact aatacacttt
     1981 cttcctgaca atggtatgct tctaaaaatt tatcaggaga aggacatgat gtttttggta
     2041 gtactttact ctcactcatt tttctataga aagtttcatt atctatttcc tttccagata
     2101 tataactttc ttctccaaaa gatacattta aaccaacaac ttctatgttg tatttttcta
     2161 ttatatcatc aggtaagtca catgcactgt ccactatcaa ttttatttta tccatattaa
     2221 cctcactata agatattatt ttatttattc agtcggtcgt acgaccgatt taattattat
     2281 tttatatatt gaatgaaaaa aagtcaatag atatagattc tcgctttatt gacaataaat
     2341 atccataagc ttataatgtt attaatttgt tataaatata catggaaagg taggttttat
     2401 gaaaacaaat aatatgaaac aggctatttt atcatctgca accaatttaa tagccaaaaa
     2461 tggagttcaa aatactagcc ttgctgatat tgcaaaagat gtaaatataa gtaaaggtac
     2521 tttgtattat cactacgcat ctaaagatga tattatatat gatattgcgg atactcattt
     2581 agaagttata actagtgcta tactaaactg tgtaaaaaat gtcaaatcaa aaaattctca
     2641 aatagaaatg gttaacttaa tcttagagaa aatatctact atagaaagtc gtggtagggt
     2701 acatatgtat cttatttgtg aggcaattac ttcaaataat gatttaaagg aaagaataag
     2761 attaaaatat attgaatgga gaactactct taaatctgaa attgttgaat cactagaaaa
     2821 atataatagt agcgaaaatg aacaggatgc tgaatcattt tcttttttac tcatgtcaat
     2881 agttgatggt ttagttgtcc aaagtttatt aaaaacagaa aaaatacctt atgaaaatat
     2941 agcttctttt ttagttaatc attggattta aacttaatac ttagataatt aataaaagtg
     3001 aactatttaa gttatctact ttaacagttc actttcttat ttattctcaa taatttttac
     3061 ttaaaacatt caatttttat ttaaaccttc atggaatcac aaaataaaaa tctcttataa
     3121 atttaaaatg tttttattta tgttcatgtg tatgcccatc tgaatgttca tgaccatgtg
     3181 tatgtgtatg ctcatggcta tgctcatgag tacctgaaaa accatcatca tgattatggt
     3241 cgtgacctac accatttaca tgctcatgtt catgcccatg actgtgagaa tgttgatgtg
     3301 aatgttcatg gttatctcct ccacagcatc cttctcctcc agtcccacaa cacccacttg
     3361 tatgtggatt atgatgattt cttactacaa atctctctct taacataatt cttatacctc
     3421 ctagtatatt attgagtact tcatgtaccc cattaactac agtatacatc taactaggta
     3481 taatctcaat acatatggca ttttttaaca tatataatta acatatataa tcttaaagaa
     3541 atttatcctt aaaacctcca ccttttactt cttcaacgtt attgacagaa ataaaagctt
     3601 cactatccat atcataaatt aaatctttaa atttacctat ttcatttgca gatattatgc
     3661 aataaatcat ctttttctta ttttgtgtat atgcaccttc tgcctccaaa aaagtgactc
     3721 ctctacccat tttattcatt atcactttgg atatttcatc atatttattt gatattaaaa
     3781 gtatagattt ttttctatca aagcaatctt ttcctatatc catagtaaaa gaaattatat
     3841 acattgtaat taaagtgtac atcaccaatt tagctccaaa taaaaaagct cctgcacata
     3901 ctacaaaaaa attaataaat aaaaatgtat ttttcatagg tatatcatac tttcttttca
     3961 ttatggctgc tattatatca agtcctccag cagtagctct tgctttaaaa gttaacccca
     4021 tacctatacc agttaatgca ccaccataaa tacactgtag catagtatca tctattggga
     4081 aatatttagc aatatcttgt gttagcccca gtgctagaga aaatagtaac atattgacaa
     4141 aacttattag caaaaattta ttatcaaaat actttctagc tataataaat attggtatgt
     4201 tcatcaagaa acttgatatt ccctgattaa taccaaataa tttgtataat ataacacaaa
     4261 ttccagctac tcctccactt aataacttgg ctggtgctag atataaattt attcctaagg
     4321 ttgatagaat catacccaat ataataagac aatattgtaa aaatatatta ttatcaatac
     4381 ttagcttttt actttcacca tacatcaaaa cgcacccctt tgcataataa ataaaccacc
     4441 ctgcataaca cacgataaat ttacaaataa atttatcaga agtagtttcc taaaaatatt
     4501 attcatcctc acttaaacgt ttattcatta atattctatc acatgacata aataatttat
     4561 atgatttata tagatttatt tattgtaaat tttggaatat taaaacattt tattgaatag
     4621 ataattatat tacataaaaa atccctatag aaatctataa actaatttct atagggattt
     4681 tggttaacta actattattt ttattttttt ataactttta tacctgtcat atggtcattt
     4741 aagttttgtt cctctaaaga tttatcatct actctgttta tttctaaagc taaagtttca
     4801 gacttgatat attcattaaa atggtcaaca gccttagcaa tttcatcatc accacagtat
     4861 tctattacta tattatctaa tacttcaaag ttattagatt ttcttaattg ctgtacttta
     4921 gatataaatt ctctagcata accttcatta attaactctt cagttagctt agtatctagt
     4981 ataacaaaaa gattgttttc aacagataca ttaaatcctt ccttagcagt tatacctatt
     5041 aatactaaat ccttattaaa ttcaaaagct tcaccatcta aatcaactgt taaagtctca
     5101 cctttttcaa gtttattagc agtttcatga gcatttaatt tacttaatgc tccaacgaat
     5161 aaattcatct tagaacctaa taatggccct gcttccttaa agtttggttt taaactaaag
     5221 ttcatatact catctaaatc cttagcaaat attacctctt taacattaag ctcttctttt
     5281 attaaatcaa ctacatcact tattgtatct tcaaacttac catctactaa cactttttgt
     5341 aatggttgac gaacttttat tctttctact tctcttgaag ctctacctaa agtaactaag
     5401 tttttagcta aatccatttt ttcttctaat ttagtatcta ctaactcttc attacattta
     5461 ggatattcag ctaaatgaac agatacttct cctgttaagt ttctatacat ttcttctgac
     5521 atatatggtg caaatggagc tatagctcta caaagaccat gtaacaattc ataagttgta
     5581 ttgtacactg cttttttatc ttctgttaat tcagttgccc agaatcttct tcttgaacgt
     5641 cttatatacc agttagataa atcatcattt ataaaatctt gtatcattct taaagtttta
     5701 tttaactcaa atatagctaa attttcttct acatctttca ttaaattatt gaacttagat
     5761 aatatccatc tatcaagttc tggtctatct ttatattcta caaaaaattc agttggattt
     5821 atatcatctg tgtttgcata taaagtaaag aagttatata cattcttcat agtacctata
     5881 aatttacttt ggatttcttt taatccatcc atatcaaatt tagttggagt ccatggtgga
     5941 gaaacatata gtaaatacca tcttaatgca tctgctccat attggtcaaa taattccatt
     6001 ggatttactg tatttcctct tgatttactc attttcttac catctttatc aagtacaaga
     6061 tctggaacta aaacactctt atatggtgct tttcctaata caaatgttga tactgcaagt
     6121 aatgagtaga accaacctct agtctggtct ataccttctg atatatagtc agctgggaac
     6181 aattggtcaa agttttcctt attttcaaat ggatagtggt gttgagcaaa tggcatagcc
     6241 ccactatcaa accaacagtc tataacctct gttactcttg tcattggttt tccacatttt
     6301 tcacatttta agtgtatatc atctacatat ggtctatgaa gttcaactgt ttctggattt
     6361 acatcttcta tagctttttc agctaattca gctcttgaac caactgaatc tgtatgacca
     6421 cattcacatc tccatatagg aagtggagtt ccccaatatc tagttcttga tattgcccag
     6481 tcatttagat tttctaacca gtttccaaat ctcttctctc ctacaaaact tgggaaccat
     6541 tttacaccat tattattttc tattaattta tctttaagtt tagtcatctc tatataccag
     6601 cttggttttg catagtatac taatggagtt tgacatctcc agcagtgtgg ataattatga
     6661 actacttttt ctttgctata taatttattt tcagaagcta gccattttat tatttcaaca
     6721 tctagcccat cttccattac aaatctacct tcccaaggag tagatgtaaa tttaccagtt
     6781 tcatctactg gttgaataac tggtaaatca tatcttttac ctgtgttgta gtcatcttca
     6841 ccaaatgctg gtgctgtatg aactatacca gttccatctt cagtagttac ataatctcct
     6901 actgtaataa aaaatgcttt tttgtcaact tctacaaaag gcattaattg ttcatattct
     6961 aaaccttcta ggtctttacc tttcatttct tctaaaactt cataatcttc acctagtact
     7021 ttatcagcta aaggtttaga tacataatat acctcatcac cttttttaac ttttaaataa
     7081 tctacatcag catttactgt taatgaaacg tttgatggta aagtccatgg agttgttgtc
     7141 catgctaaga agtattcatc agcatctttt ttcttaaatt tagctataac tgtattagtt
     7201 tttacttctt tatatccttg tgcaacctca tgactagcaa gaccagttcc acatcttggg
     7261 caataaggaa gtattttatg tccttcatat atatatcctt ctttgttgaa tttgtttagt
     7321 atccaccata cagactctat atagttatta tctaaagtta tatatggatt atctaaatca
     7381 acttcataag ccattctctc agtcattatt ctccattgtt tttcaaatga gaaaactgac
     7441 tctctacatt tttggttaaa ttcagctatt ccatattctt ctatttgttg cttgctagta
     7501 agacctaact ctttctctac ttgtatttca actggtaatc catgtgtatc ccaaccagct
     7561 tttctcttta cttgataacc agacattgtt ttatatctac aaactgaatc tttaagcgtt
     7621 ctagataaaa catggtgtac tccaggattt ccattagctg ttggaggtcc ttcatagaat
     7681 acgaaacttg ggtcatcttt ccctttttct aaagtttttt caagtatatt tatgtctttc
     7741 caataatcaa aaacttgtgc ttctgcttgt tttacagaac tgtctaccaa tggcttaaat
     7801 ttcgccatgt aaatcaccct ttcctactaa atattataat tccaaaattt tttattcttt
     7861 cacataaatt taaatactaa aaaactcgcc ccctttaaac caaagggacg agttataaat
     7921 cgcgttacca ccctaattct atatgtaaac taaatactag ctacatataa cactctaata
     7981 gtataacggt actaaccggc acagcttagt agaatttaat cgttcagcct gcagctcaag
     8041 agtgattttt atctttataa attcattggt tctcagcata cccaattctc tgtaaaatta
     8101 tctaaagata aatgtctcta tcaacgcttt taaaaattta tctgtgttta tatgaaattc
     8161 tataacaaat aatttgaata gtcaatatat ttttgaaaaa tataataatt tctatttttc
     8221 ttacattttt atcctatttt taatctttgt tatagatttc tcaaactcac acaattattc
     8281 tacataaaaa ataaacaaat tacaagctta ttctaaagtt gtagcagctt catcattact
     8341 ataattatac actgttgttc cttcaaatgc atttacacat ttttcatcta catcataaaa
     8401 aatctcatca acacttctta tttcatcttc taacagtgat ttaaatttat ttctaaatat
     8461 cttaaattct ttgactaaag catcatactc ttttcttatc tctataacac ggttattagc
     8521 ttgttctata atttgtctag cttttaaatc agcttcttca acaattatct tagccttttt
     8581 attagccgcg ctacaagtgt cttctgctgc actttgagca gttattaaag tagcttttag
     8641 tgtttcttca atattttcat acttgtttac ttggtcttga tataacccta atttttcttt
     8701 aagagctgta ttttccctac atagagattc ataatcttcc ttgatgatat ctaaaaattc
     8761 atcaacctct tcatctctat accctcttaa acctttttta aactctttat tttctatctc
     8821 aattggagtt agcattacat ttcctcccag ctataattta aattagtcct tatacaatta
     8881 atcttgcttg aacttttatt tttccttttt tagtaatatc tcctatttgt gttaccttag
     8941 ctcttccttt gcctctaata gaaatcaaat catcgttttt tactatttta gaagttgaag
     9001 ttatcttttc ataattgaca tggacttttt ctccatttat gtattttgca ctatcttgtc
     9061 tagatatatt gtaaatacca cttatcacac aatctaacct atctgaagat actgtaaatg
     9121 atacttcttt atattttgaa gaactattaa caatattttc tctggaaatt tcctttacaa
     9181 ttacattatt tcttgaaacc ttttctaagt ttacaattat aaaatcgcaa atatcactac
     9241 ttacaactac ttgacaaaag ctatcatgta taattatatc tccaattttt tctcttttta
     9301 tacctagacc cagtattgac cctaagtaat ctttatgaga tatattttta aatttgaaat
     9361 tcccttctat ttgtaaaaat ctaagagtat cttctatatc ttcatattcc atgtaaaatg
     9421 gataaataaa tacagtgctt ctttcagctt gttcataacc tccatccaca ctgtatttta
     9481 tttcatttgt agaatttaat attgctacag catttttcac ttcatatgga ttaagaaact
     9541 ccgtagactt aacatcatag ttttttatac aactattagc cttgtctatc actttaaaca
     9601 ttttattttt caagtctata tcctttatgt gatttgttag ttttaattta tccatcctat
     9661 caccttagaa tataaccaat actaattgtc ttaggaacat caataatact attgcaatta
     9721 aaggagaaaa atctataggt ccatttgtat atttatacat tatagacctt attggccctt
     9781 ctattggctc tgtaagattt tccaaaatat caaaaatttt actttgtctt ccatttggaa
     9841 accaagtcat caagcttttt ataactatca tccatgctat tatatctaaa agataataaa
     9901 gtgcaattct tattgttccc atatattaac cgccttttta ctatttattt tgccaaggga
     9961 agaatgcttt actttctaat tcttttttca tatttgcatc tatatccaca ttattaggag
    10021 ctagtataaa tattgattta gaaacctttt gtatacttcc atctaagaca taaactgccc
    10081 cattcataaa gttaaatata gatttacgaa cttcagaatt agtcaatcct tccaaattca
    10141 ctataactgc ccttctttgt tttagatggt ctgctatagt tgcagcttca tcataaacct
    10201 taggttcaac tattacaact ttcatttggc tagttgtatg taaatttaca attttatttg
    10261 cttttgcagt actaaagcca ctgttcattt cttcttcttg aactatatcg tccatgccac
    10321 tttcatattc atcttcaatg taatcttctt cttcatcaac tatccaattc ttaaatttag
    10381 atattattcc atttgacatt attaacttcc tccttaaaat ttattcaaat ttgagtaatt
    10441 attttttatt gtagtttctt tctccaaaga ttgatgttcc aactctaaca atggtagccc
    10501 cttcctctat agcaatttca taatcgtgac tcattcccat tgacaaaaca tccatcccta
    10561 cattagggat gtttagttct tttatttgtt ttgataaatc tttaagacct ttaaatacac
    10621 ctcttatctc atcttcatct tctataaaag gtgccattgt cattagaccc tttattttta
    10681 tatttttata tttttctgac acttctttga caaagtctac aacttcttct ctatcaagtc
    10741 catgcttaga ctcttcttta gatatattca cttgtactaa gcaatctatt tctctgttta
    10801 tttgttctgc acgtttttga atttcttcgc ataatgcaat tctatctaaa gagtgtatca
    10861 tacaaacttt atcgattatg tacttaactt tattggtttg caaagttcct atcaggtgcc
    10921 agtttaattt atcacctata atatcatact ttctagctaa ttcttggggt ttattttcac
    10981 caacagaagt gattccttcc ctcattgcct cattaactaa gtctacatca acagtctttg
    11041 tgactgccaa aagggtaatc tctttgctgt ctctatctac tttttttgca gctttatcaa
    11101 ttttgtttct tatctcagac aaattatctt taatagaacc cattattatc acctacttta
    11161 tccttatatt tgtgtttata ctgttaggtc ttaaaattat tttgtcatat agattaacac
    11221 ttgtaactcc atttatgtca tttttataat aatccacaaa tacagaagat tcattctcat
    11281 aagttattcc ttctaatatg acaaatttta cactttgtga ttgttcatca attacatata
    11341 caccacgttt attgtctttt gtttttatag aagttttagg tatttttaaa cattccatct
    11401 gattatatat tatatcaaat tcttcgacac ttgtatcctg atttgcaaca atttggttac
    11461 ttatttttaa ttttaccatt attttattat tattttcata tattttgtaa acttttgcat
    11521 ctagtttttg ctcatgatat tcaattatta cattttgatt tttttcaaag ttattagaat
    11581 cttctttact gatgtatgta gatagataat tactttggtc attggcatct ttttctatag
    11641 ttccttttac atgaatcttt gcatcaactt tttcattttt taacaccaac gtatctacat
    11701 ttttgctaat tatcattact aatatattaa atactaaata tataagaata ccaagtatca
    11761 ctaatctggt atattttacc tttttcaaag actctccccc tataaaatta ctttcattat
    11821 aagtacttct tctaaaaaat gtttattcct tttttataag ataatatttt acgttctaat
    11881 aattattttt tccatattaa atttattttt tatcaatctt cctatacaag actgcattat
    11941 ttatatataa ttgtattata aagtataagt ttaatttaca ttttatacgt taagcttact
    12001 ttatatattt ttatagttat ttttattttt agatgcaata taaaaccaat taagatttaa
    12061 aataactttt gttatgttga catttattta ggtaatcatt tatgaattag gataacgtta
    12121 tcacaacaaa agtttatgtc ctaaaaaaaa tacaaggagg attttcttat gtcagaaatt
    12181 ttattaggaa caggtttact tttattggtt ctatgttgtt tcacaatttt tagttacaag
    12241 gccccatatg gtatgaaggc tatgggagct ctagcaaacg ctgcatgtgc tactttctta
    12301 gtagaggcat tcaacctttc tttacttggt gatgttttag gtatggaatt ttttagaagt
    12361 gttggagctg ctaatggtag tcttggtgga gttgctgctg ctatacttgt gcctttagcc
    12421 ttaggtgtat ctccagttta tgcagtttta ataggattat cttgtttaga tttttctata
    12481 ttatctggat tcgttgctgg atactttgtt tctttcttaa ttaagtttat agagaaaaaa
    12541 gtgccacctg gagtggattt agtggttgta attcttctat gtgcaccact atctcgtctt
    12601 atagcatcaa atgtagaccc attagttaat agtacactac tacaaattgg gcaagttttg
    12661 atagaagcta ccaacattag ccctataata atgggaatta tactaggagg tctaataaca
    12721 gtagttggaa cgtctccact tagttcaatg gctcttacat ctataattgg tcttactggt
    12781 cttcctatgg caataggagc attaggtgct tttggttcag cttttatgaa ctcaacacta
    12841 tttgcaaaat tgaaattagg ttctaaaaaa gatattatat ctgttgctat tgaaccatta
    12901 acccaatcag acataatttc agctaatcca atacctatat attctactaa tttctttgga
    12961 ggagcctttt ctggtatcat tgtagccttg atgggtcttg taaacatgac accaggtaca
    13021 gctacacctg tggctggttt tgctataatg tttgcttata atccagttca acaagttctt
    13081 ataacagctg gtttatgtgc tttattcaat gtaattgcag gatttttagg ttcaaaaata
    13141 ttcagtacct ttaaagttct tactgctggt gaaatacgtg gtactgatgt agaagaagaa
    13201 attgcttaaa aaacaataag atttataaat ttatatctca agtagtagag ttttatattg
    13261 taggcaattt tattaattgc aaacgtttta ttttttattt atgtttatga ttattatgtg
    13321 tgttgaatat ttagataaat ttttatttat tatttttatt tagatttttg tttagttttt
    13381 tatttggttg atatattagg aataaaaatt agtatagttt attactagtt agatggcata
    13441 actttttaga tacataaaaa attttatatt aattagatat tattaaaata aaagcgatac
    13501 atgaattttt aaatgtccat gtatcgcttt atttttgcca taatcttaat aactgatttt
    13561 taaagtttat tatcttaaca aagttcatta tcttatataa tatttaaatg cataggcttc
    13621 tttaatttaa ccatctaaat atttaattaa atattattat atgtaatata actaattttt
    13681 aaatgaatgt ataggagctg gaatccttcc tcctctttga gtaaataatt cagatgaatt
    13741 tgtatttata ggcattactg gtgctcgtcc taaaagtcct ccaaattcta ccatatcacc
    13801 tacatcacat cctggggctg gtataattct aacggctgta gtcttattat taatcatacc
    13861 tattgctgcc tcatcagcta tcatggctcc aagcgtacct gctgtagtat ttcctggcac
    13921 agctatcata tcaagaccta cagaacaaat agcagtcatt gcttcaagct tatctatact
    13981 taaagcacct ttaataacag catcaatcat acctgcatct tcagatactg gtataaacgc
    14041 tccactaagt cctccaacat gtgaacatgc cattactcca ccttttttaa cagcatcatt
    14101 taaaagtgct aatgctgctg tagtcccatg agtaccaaca acttcaagac ccatttcctc
    14161 taatatattt gctactgaat caccaatagc aggtgtagga gctagtgata agtctattat
    14221 cccaaatgga acatccaatc tactagaagc ttcctttgcc acaagttgtc caactcttgt
    14281 aattttaaat gcagtctttt taactgtttc tgaaacaaca tcaaaaggct ctccctttac
    14341 tttctctaaa gctctcttaa caacaccagg gccacttaca ccaacattta taattctatc
    14401 agcctcacca acaccatgga aagctcctgc cataaatgga ttatcttcta ctgcattaca
    14461 aaatactact aattttgcac aaccaaaacc ttttgcatct tttgttaatt ctgcagtttc
    14521 ttttatgatt tctcccattt ctttcactgc atccatattt ataccacatc ttgtagaacc
    14581 tacatttact gaagcacata ctttattagt acttgctaga gcttttggaa ttgacttaat
    14641 tagtatctta tcaccttttg tatatccctt ttgaactaat gctgagaatc caccaataaa
    14701 atctatacct aaatcctctg cagccttatc caatgtctgt gcaaacttta catagtcatt
    14761 ttcatctgtt gcacctgcaa taatggaaat tggagttact gatacccttt tatttacaat
    14821 tgggatacca aattcacgct ctatatcttt tccaaccttc accaaatctt tagcagaact
    14881 cataatctta ttgtatattt tttctctagc tttatctcca tcagggtcta tacaatctaa
    14941 taaagaaatc cccatagtta tagttcttat atccaactgt tcttcatcta tcatctttat
    15001 tgtttcaagt atatttccta cattcactat tatcacctcc ccttactgtc tactacagat
    15061 tatgcattga attaaatatt tcttctcgct gaatttttac atccactgat aatttcttac
    15121 ctctttcaac caagtcctca accgttttct caaaagaatt taatgatttc tctaaatcaa
    15181 ctatcataat catagtaaaa taatcctgta atattttttg tgatatatct actatgttga
    15241 tattttgctt gtataattca tcactaattc ctgccactat acctggttta tcctttccaa
    15301 ttactgttaa aattgctttc ataataatat cctcccttat ttttttataa atcttatatt
    15361 tttacaaatt gaactatgtt ttaacatata tttcatttat ttgttagtaa accacttttt
    15421 atgtatatat ttaatatatt ttaataccta tacattcatt tgtcaatttt ttttcaatat
    15481 tctattattt ttatactatt tagaaaattc atatattata tatgttttct caacaataaa
    15541 tacagttttc ataataatga aaggtattac ttagcaatcg catgattttc taaataatac
    15601 ctttctaaaa attatttttt taattttatg aatttaatta tactttttct agtatcagga
    15661 tttacaatta atatagaacc aatcataatt ggtgttagta tacaaaataa aaaaataata
    15721 tcttttttta gattatattc tttagattct aatttataat tattatttat tattccatca
    15781 taatcactta tatttgactt atttttattt gtatcttgat ttttgttaga aatactttcc
    15841 ctataaggaa aagaatgttc attggtacca aatactaatt ttgagcttaa atcataatta
    15901 tcatcatttt ctgtaattga atttttattg taaaatcctt tttctttatc agaattattt
    15961 tctttattac cgtctttagg tatatcatga ggatttttat tttcattgct atcagtactt
    16021 gattctacat catctttagg agtatttggt tgtttctcat cagaattatc attttcatta
    16081 ttattatttt cattattatt gttttgattg ttattactat cattattttc atttccatta
    16141 ttcccatctt gattgttatc atcctcatct ctatccgggt cgtctctacc tcctcctcca
    16201 cggtcaccat caatatcatc attttcccgt tcatcatctg gtgtctcttg gttgtctccg
    16261 cctcccatta taaatcctgg tttaaaaact atatttgaat acccttcttt taatcctcct
    16321 accactttta ctctaaaaca acgcaactta aaatcttcaa aaaaaagtat agcttgatct
    16381 ctaatatcct cctgttcttc agatatccaa ttgattccat cgcttgaata ttcaacaaaa
    16441 accttacttg cactatacgg catatcataa aataaaacag ctacatatcc attttttgta
    16501 ttttgaaatt ccagttccaa attgtctatt ggttttttct ctataacaga tacattcaaa
    16561 ataggaacta catcatcatt tattataatt ttttctttat ttaacatttc tgttttaaac
    16621 tttcctgtta tattaaaatt ttctcccttt tccaactttt cataataatc agtaaaatcc
    16681 cattctacat ctatatcaag tcgattacca ctagatgttt ctaataccat atatttggga
    16741 aattttatat tttctatttt atcatcttta cttactttta tttcatcact aaatacacca
    16801 attcccctat gacaacattt cacaatatca tagccagaat cttcagctat agagggtata
    16861 agctcattat aatcatcaat tatataaact ttcttacttt ctgatataat tgattgtgcc
    16921 aacttattat tatcttttag taacccactt tcattgttgc tactatatgc ttttattgaa
    16981 gtttcttcta tctcaacatc acctttagaa taaacaccta tagcatgttc tccattcact
    17041 tcaatatcct tatttagtat tctaatatca ttttcagtat aaattcctat tgcatctttc
    17101 ccatttgcct ttattttttc aacagattcg taggatgatt taatatgtaa gtctcctcct
    17161 tcttcaacgt ataaggctgt cccatcagta gcaattatgt tgtttatacc aatacttata
    17221 cttccaccat tttctatatg tattactcct tcttttcctc cttcacctat tatattcaat
    17281 tcattatcat caataataaa tctcccatga ttttttatca atattttata tttgttggta
    17341 tctattgtaa aattactgtc ataaggaata taaaatctaa actcttcatc tattaccata
    17401 tcacaagaaa gagtataaac tatttcttct ttattatttt tattttcttg aacccacctt
    17461 tcaaaatctt caagacaatt ttcctcttgt ccaaaaactt tatgtggaaa acttataaaa
    17521 cacaaaacta ttatactcat aaaaataaat atctcatact ttttcatctt acactcctag
    17581 aaatttttta gctttgaaac aatctgtcga aacatacact tcacccacta taatttttcc
    17641 acaattatca attattgtca atttataaaa gtatctttat taattgttat aaataaaaag
    17701 ctacttacta aagtaaacag acgtttcact ttaataagta gctatatttt tataacaaaa
    17761 tagttataat attagaactt ccttcattta caattttttg taaagtcttt tgcattttaa
    17821 ctcttatctc ttctggcata gtatataatt tactttgtaa ttgctctttt actaagtcat
    17881 gtagtgattt accaaacata tttgattccc ataaatctgc tggttgctct tcaaatactt
    17941 ccataagata ttttatcatc tcttcacctt gattttgatt tccaactatt ggagaaacct
    18001 ctgtagatat atctgctttt ataatatgaa gtgatggtgc attggctttt aacttgatac
    18061 catattgttt tccttgtttc attatttctg gttcttccag actaagttct tctaaggaag
    18121 gagctactac tccatatcct tttatcttgg catctattaa agcactttct attttatcat
    18181 actcattttt aactttagat agtctagtta tcagactaag cagttgataa tctccttcaa
    18241 ttttaaatcc acttttttct tcaaggacat tatagaataa atcttgcttt gtctgtaaat
    18301 caatatttat aacaccttct cctagctcta cattatcaac acctgtatct tccaaaaact
    18361 ctaactcaga aaatccttgt ataatacctt ctatatccct tattttacct atatctataa
    18421 tactttgttt taatgttgtt attatgctac tttttatcca atgatttctt tcaagacctt
    18481 caacccattt tggtaagttg attcttattt ctgtaagtgg aaaatcatat aatacagctt
    18541 ccataacctc ctctatatct tcttcttcca tttcaaccac attcattgga agaactggaa
    18601 cttcatactt ttcttccaat tcacttctaa gcatactcgt ttcctcactt tttggactta
    18661 acgtatttaa aaccactgca aatggttttt taagattttt caattcttgt ataactctct
    18721 cttctggttc tacatagctt tttctgtcaa ttccagtaac tgaaccatct gtcaagacaa
    18781 ctatccctat tgtagaatgg tcttttataa cttttttagt tcctatctct gctgcctttt
    18841 caaatgtcat agcttcttgt gaccaaggag tggatactaa tctttgtttt ccaccttcct
    18901 catgccctaa agctccttca actatgtatc caacacaatc taccattctt acttttaaag
    18961 atactgtgtc ttttattttt atttctacac catctgctgg tacaaatttt ggttctactg
    19021 tcattatcgt tttacctgaa ccactttggg gtatctcatc tcttgttcta tcctttttaa
    19081 attcattgtc tatattaggt ataaccaact tttccataaa ttttcttata aaagttgatt
    19141 ttcctgttct tacaggtcca actactccaa tatatatatc cccctgagtc cttttggata
    19201 tatcttcgta tatgttatta ttcatattat tcctcctata catctaatct tattaaatta
    19261 tatttagagt taagggttaa tattcttaaa atagaaaaat aaagaccagc aatattaatt
    19321 taatttatat atagaaattt gtaaatttca atgtaaatta aattaatatt gctggtccaa
    19381 atagtagtgt attatattaa ctgactttag tagtattttc atttgaatta ttattcatct
    19441 caataagttc tttacgtctt tcttttgtca tgttcatcca tataaatgta aacaccaatc
    19501 caattatggc tactatagta agaactatga agtagtacct atatccagtt acacccggaa
    19561 atgcatccaa tatcttccct gctgctaaag gacaaagtac ttctggaaga tatcctaacg
    19621 tagcaactat tcctatagca gttcctgata ttgataatga ataattacct tcttcaagta
    19681 acgaatagtg taaaccttga attgcataca tagatacata tattgcaaca catccaatca
    19741 atagtatata aatcatagat gattttgttg gtgtaaatat tacacctaaa agacctgcaa
    19801 tcataagtat aaatccataa gatataatct ttgatgaacc aactttatct ccaagtaatc
    19861 cagaagcagc tgatgctact ggtctacaat actgagctaa tattgagatt gcagatgcaa
    19921 acaccacagt agctccaaat gcttcagttg aataaggcgt aaaataataa aatgacatat
    19981 tagctgaata tgaacaaaac attattattg ttattatcca tgtatgaggc atttttatta
    20041 ctgcaaaaaa ggcttcttta tcaaattttg ctgatacctc tttttcatta tcctttaatt
    20101 tgaaaaatac taatactcca actattacat ttattgctga gtataatact ataatagctg
    20161 taagaccaga ttcatcactt acttttgatg aaatataccc aaatagtatc aatactagag
    20221 atagatgaat tgcattggta attccacgac cactttccat aaatccaaaa gcttttcctt
    20281 gttcattttc agatgctaac attctaacag cttttacaag agctggccaa aaagtaagta
    20341 ttgttgttat tccccataag gcatggatta aaaacacaac aatatatgga ggataaagtg
    20401 ttaatacaag accagatatt cctgtaataa ttaaagaacc tgaaagcaat tttctggctg
    20461 aaatacggtc tgctaaaaaa ccaccaaaaa ggtatgatac cattgcaagt agaccataaa
    20521 tgcttcctaa gcttcccatt tgtgtatttg taagattaaa tgcttgtgca aaagtatcat
    20581 aataataatt ctttaaatat ggtaaagcat atataaatgc tcctgctaca aagagtatta
    20641 tcaagacttt gaaatttttc caaatgcttg gtttatttac ttcaactgac atacttcctc
    20701 cttatgtaaa tacctgtaaa ttttcataat aattaattat tgcttatttt attgtttatt
    20761 caatatttag gaatatttat taatatatta ggttaatcta tcaataattt atggtgagtt
    20821 atgagctaaa agtaaactct tttagctcac cataataatt atatttttat ttatactaac
    20881 atccagagtt attagatatc tttttttcat agttcttcca tagcattaaa cctgttatta
    20941 taacaaaaag acctcctcct atcatttcaa tctcatagat tatattatct tttaaagtct
    21001 ccataactgg tgccgttgag aaaagtatgc caactacagt tactacaagt accattctgg
    21061 caatattgat acaaactgaa tcactttttg caattgaata tggtctttct tcatttggtc
    21121 ttgtctttct aagtctaata taagatgtaa aaagcaagac atatggaaat aatgctgtta
    21181 atgctgtcat agtaacaaga acattgtata tattatctac tgatggtaat aagtttgttc
    21241 ctacaagaag tagactcact attatagcct gaactattac tgcatttgat ggtatattgt
    21301 atttatttgt aacagtcaat tgcttaggaa atacaccttc tcttacattt ccaaaaagca
    21361 tctttactgg acctgctata taaagtatta atgctcctag tactgataaa gttattccaa
    21421 atgctactat tcttataaac caacttccta tacctaaatc ttgtgcaact ttagctaatg
    21481 catctagtat ccctgtagat gctgcaattt tatctggagg aagtatcatt gttattgcta
    21541 ctgaacctaa gatatagatt cctcctatta aaacagccac agttaatata gctttaggaa
    21601 agtttttctt agcattatcc atttcggtta tgaaattagc agtcgtttca gctcctgata
    21661 atgcaaatat tatagcagat atagaaacta atgaattggc atctattttt ggtattatat
    21721 tttggattgt atatatcgat gctgatggag cttttttgag aataactaca gacataaatg
    21781 ccatcactat aagtaatata gcaggaattg tagaacctaa tgctcctgta ttcgtaaaaa
    21841 attttccaaa tgccatccca cgagtactta ctaaggacaa cagccaaaat attatgagtg
    21901 aaagtgctaa aataaacatc ttattgtttg ataaatctgg atttccaagc atatatgcaa
    21961 tatttatcgc taaaaaagtc aagaaagaag aataccaaaa tatctttgat gtccaattta
    22021 gccatgacac cataaatccc cacttctctc cataagcttg tttaacccat tctccaagtc
    22081 caccatcttt tggatatgtt gcagccaact ctgcacatat aagtgatgca ggaacaaaga
    22141 atataaaagc aaacaataac caaactggta tagaaccaag acccaatcca aaactagagg
    22201 cagtagattt agctatccat ctaatcccaa atagtgctga tatattcata aatactaaat
    22261 cccaaaatcc aattttcttt ttcactgtaa ttcctccaat ttcaaattta ttcagactca
    22321 tacacaatgt attgacttaa atatcaagta taagttcttc aacacttctt ggataattag
    22381 ttataattgt agcaccattc ttacctacta ttgctgtatc tgagtgtcta aatcctccaa
    22441 ctccaggtat atatatccct ggctccatag aaaataccat tccttcttct aatattaatt
    22501 cattgtcaaa tcttaaatat ggctcttcat gttctgataa acctaaacca tgaccaattc
    22561 tatgatttga atataactct agaccatact ttgcaacaac agctcttgct gcttcatcca
    22621 ccattcttgc tggtatacca gcttttattg tatctaatcc agcttgttga gcctctactg
    22681 caatcttaaa cacttctttt tgtctttctg ttggtttgcc tataataaaa gttctttcat
    22741 tttctgctct atagttttca taccaaacct gtctactatg tattactata tctcctcttt
    22801 gaagtattct agtattggaa tctaaatgag gttgtgctgt tctatcaatc cctgaacaag
    22861 tccagtttgc aaatcctata tatgtatcag gataattttc agaaacatat tttagtaaag
    22921 cattatcccc tgcaacgtca aactccaatt cactcatccc aactctaaca ttttctaaag
    22981 acccttttat tcctatatca gataggtaac ctgctatctt caaaaattct atttcttttg
    23041 catcttttac atatctcatt tcaaatattt tacttccaac atctttgata gcaaatgact
    23101 tatcagttat atactctgaa aagcttgcag atactatatc tttttctatt ccaacagttg
    23161 ttcctttagg gtatttattt aaaattatat ctaagtaatg tttatgtgat attccatgtt
    23221 ctttcatttc tggtatttca taatacacat atacattatc aacttttgca acttctttag
    23281 catgtaattc ttctaatcca ggtactatta attctatttt atcttgagtt actactgtta
    23341 caattggtct agaataagtt attgctttaa atccactaat ataaaattga ttgtctggtt
    23401 caaatattaa agcaatttca atgctttttt cattcatgta ctttctcaat ctttctagtc
    23461 tcatattatt tgacataatg cacctctttc ttaatattca gataatttat tcaatacaac
    23521 aaggatgaat atattatttt aaaattctta atatttttag tacttgtcgt atttgtatgt
    23581 aaattaattt tatttgtgat tattttatca taatcatgat tattttgtca atcatgattt
    23641 tattttaaaa aattttttat attctagtga ttttaaatta tctaaaatat aacgagtata
    23701 cattgtattt tgtaatatta ttcttgattt atggtaagtt taatgataaa atcacgatta
    23761 tataaactat attgattatt tgtaggtgaa taaaatgaca aataaagaat tacaaaagaa
    23821 aaggatattg atgtatttca tagaagctgc tcaaaatatt atggaaaacg aaggtataga
    23881 aaatattaca cttcgtaagg ttgcagatat ggctggctat aatagttcaa ctctatataa
    23941 atactttaaa aatttagacc atttaatatc atttgcatct ataaaatatt ttaaagatta
    24001 taacttgaat atttcaagat gtatagaaaa tgtcaatgat gaatataaga aatatataat
    24061 tatgtggaaa ttattttgta agcattcttt tgataatgct caagcttttt accaggtatt
    24121 ttttaacttg tctagtgatg aacttagcta tataacaaaa caatattatg atatgtttcc
    24181 agaagattta ggtattcatg atagtgacat atctttaatg attacaggag aaagtataaa
    24241 ggaaagaaat aagatactat taaacaatct tgtagatact ggatatatac cagctaaaga
    24301 tttagacata atcaatgata taattatttc tttttatcaa aatctattat tcttaaaaaa
    24361 gaacacagga aaaaattctg atgatgatga attaactatg aaaatgattt cttctattga
    24421 atacataata aaacatgcta aataaaatta aaatatggct gtctcaaaat agagataagc
    24481 cttttttatt gttgaaataa taaaaatata tttaatttca tataaaaaag agtgcctcgt
    24541 aaactaaaaa gttcattttg agacacccct aaaataaatt tattatttat tatacaatag
    24601 tttatatatc atcacctaaa actacttctt ccatttcatg ctttttactt ctcatcatta
    24661 gttctattcc aacttcttta ggatttgaac ctttctttat tacagaatat atagcattag
    24721 ttataggcat atctatatct aatttttcag caacatcatg tgctacttca gtagcagtta
    24781 tgccttctac aaccatcttt acttctttta aagtatcttc taaactcatc cctttaccta
    24841 tgagtatacc agctcttcta tttctactat gcatacttgt acaagtaact attaaatctc
    24901 caatacctga aagacctgca aaggtagaca tattagctcc catagcaatt ccaagtctgc
    24961 tcatttcact aatacctctt gtcataaggg cagcttttgc attatcacca taccctaatc
    25021 cgtcacatat cccagctcca aatgctataa tattttttaa tgctcctcca agttctactc
    25081 caactatgtc tggattcgta taaactctaa gtttagggct cataaataaa tcttgtatca
    25141 tctgtgctat ttttaaatct ttagaagcaa ctactacggt agtaggaata tcccttgcta
    25201 cttcttcagc atgagatggc ccagatagta ttacatatgg attttgtggt aattcttctt
    25261 cacagacttg agataatcta agcccagtcc ctttttcaag acctttagct acattaacaa
    25321 taacttgtcc ttcttttata aatggtttta ttttttggca agtacttctt acagcctgtg
    25381 atggaactgc taaaactatt accttacaat cttttatagt tttttctata tcagtactta
    25441 tagttatatt atttggaaat aatactcctg gtaaatagtc tgtattttct ttagttctgt
    25501 ttattttttt agcttgttcc tcttttcgag tccacatgct cacatcatta cctttttttg
    25561 caagccctaa agctaatgca cttccccagc ttccagtacc taatacacat actttctcca
    25621 tgtttaacac cacctgagta taatatattt actattcttt cttttgacca atttttctct
    25681 ctgtcccatt taataatcgc tttatatttg ctctgtgatt atatattact gatgcagtta
    25741 agaacagagc tactattaaa ccagctttat tttttaccat aatcataaat attggtgaac
    25801 atcctattcc tacaacagaa cccaatgaaa catactttgt tatagctacc accaatataa
    25861 atactgctag acacattagt gttattactg gatttacagc tagcattgcc cctagtgaag
    25921 ttgcaacacc tttgcctcct ctaaatccta aaaccgctgg ccaattatga cctgctacaa
    25981 cacatataac tgccagatat ccagctaaaa gagtatctat tccaaccaat cttgctgcaa
    26041 attctgatat taaaacagcc attacacctt taagaacatc acctaaaaat gtcattgctc
    26101 ctgctctttt tccaagtgtc ctaagtacat ttgtcgaacc tgcattacca gaaccttgag
    26161 tccttatatc aaccccagca attcgctttg caactatgta tgaggtagat atatttccta
    26221 aaagatatgc aactacagca ataattatat aactaaatat ctccactgaa tttcccccct
    26281 ttagttatat tttaagatat ttatttttta tttttttgtc tatactcaaa ctttatggaa
    26341 gttccttcaa atccaaaatt ttctcttatc ttattctcaa ggtatctttg atatgagaag
    26401 tgagttaaat ccttatcatt tataaacaat gtaattttag gaggtctaat gtcagtttga
    26461 gtcccatagt aaatttttaa tcttctacct ttgtcagatg gtggttgatt taacattaca
    26521 gcttctccaa ttacttcatt cagtgctgaa gttgatattc tcttagaatg ttcatttgac
    26581 acatattcaa ctgtatctag tatcttgttc attctttgat tagtttttgc agaaacaaat
    26641 acgattggag catacatcat aaatggaaat ttttctttta tatcttttgt gtaattgctc
    26701 atagtcttat tatctttctc tattaaatcc catttattga ttacaaatat gcatcctttt
    26761 ccttcatcat gtgctatacc tgcaaccttt gtatcttgtt ctgttacacc ttctaaggca
    26821 tctattacta tcaatactac atctgctcta tctacagcac tcatagccct tattacactg
    26881 tatttttcta ttgtctcata tattttactt tttcttctaa gccctgctgt atctattaga
    26941 agaaatttct gtccattttt ttcaaaataa gtatcaattg cgtctctagt agttcctgca
    27001 attggactta caattactct ttcttctcca agtatcttat tgagtataga actttttcct
    27061 gcatttggtt tacctgttat agctactctt attatatctt cttcatactc tgtatctaat
    27121 cctgctggaa aattttcaac tatctcatct aataaatctc caaatcccat actattggct
    27181 cctgaaactg caaaaggcgt tccaaatccc aactcataaa aatcgtatat attatcaaat
    27241 tgactttgac tatcaatctt attaaccact aagattactg gtttttttgt ctttctaagc
    27301 aattgtgcaa tttctttatc agcagctgtt attccagctt taccatcaac tataaaaagt
    27361 attacatgtg acatgtccat tgcaagcatg gcttgatttc tcatttgaga taatattata
    27421 tcttcgctat caggttcaat tcctcctgta tccactaatg taaaatattt atctaaccat
    27481 tctacttcag caaatattct atctcttgta actccaggtg tattttcaac tattgatatc
    27541 ctttttcctg caaatttatt aaatattgta gacttaccaa cgtttggtct cccaactaca
    27601 gctacaactg gcctacttat actcatttta tttctccttc catcttttgt tttctatttt
    27661 aaaattttat ccacaaaatc ttttccttca ttcaaacaag ggataatttt tattctcata
    27721 agttcttcta attgatttaa ttcaatatta tctaagaaaa tttcttcatc tgcttttagc
    27781 atacttcttg ttatataaag agcttctcct aattctttat cttcaagttg ttcttttaaa
    27841 tccttagctg ttaaaagacc tgatacagtt attgtctctc caaaaaactt gtttttgatt
    27901 tcatatacat ttacttgaag atttttaaac ttgtccatca tagcatctgc catactctgt
    27961 ataaattcat atgcagaatg accagtagct attgatactt tcttctctct ttttagaatc
    28021 ttctcaggca atatatttag atactcttca atctctgttt ttaacttacg tatcatacct
    28081 acaccatctt caaactgtaa aaaaccttca tattcttcat atccaggcaa ttcactatta
    28141 gatagtatat aaaattcatc tgataaaaat gcaaatcgag tacctaattt ttcaagatat
    28201 ttctgttgta tttgatgtat ttgctctata gttttacttg cgctcttatc attaaatata
    28261 tttaattcta ctagattttc tctatgcttt gtaatcccaa ctggaactat cgcaacactg
    28321 tttacatacg gataaagttg agctagttct tttattgttc tatccaattc ctttccatca
    28381 tttatacctg gacataaaac aatttgacag ttcatttcta tatgagcatc tgccaatctc
    28441 ttcattatac catataattt tcctgcaaat ttattgctta tcattttttg tcttaactct
    28501 ggattagttg tatgcacaga tatatttata ggacttattc tatattttat aatattattt
    28561 atatcttctt cactcatatt tgtaagtgtg acaaaatttc cttgtaaaaa agataatctc
    28621 gaatcatcat ctttaaaata aagagtttct ctcatacctt ctggcaattg gtctataaaa
    28681 caaaacatac acttatttct acagctttta gcttggtcaa ttatcggatt tgtaaattca
    28741 acacccaaat cttcatcata gtctttttct atctcgtata tgtagacttc gccatctttt
    28801 ttttgtattt ctacatctaa atattcatca cttagtaaga atctatactc tattatatca
    28861 tgtattggct gttcattcac agatataagt aaatctccta cctctatacc catttcttct
    28921 gcaatactat ctttataaac cttacttata atattattta ctttttttac atttacttcc
    28981 aatattttca ccactctttt gtattcatca atgcttatta taatgtattt taatatgcaa
    29041 tttatttatt tattatattt tatgatagca tttttaagca tttcatgtca taataacaca
    29101 ttttaattct aatagtcaat ataagtaaat agctataaaa tagattagaa caaataatat
    29161 tcaaatttaa gtttcttaaa aatttaaata ttatttgttc ttaaaaaaat tttataaatt
    29221 taatgtttta ctatgattaa cgtcccattt gtcatatcat gaaccattcc acctacacac
    29281 tttgacatca acaatgcctt attttgtaag tctttctcat catttgcata aaatattgga
    29341 caacctccac catttttcat attcttatca agtgtaacta tagctagggt atattcattt
    29401 aaacctatat ccattcccat ctcatgcact tcctttcttt cctattttat aattttttag
    29461 tgataagttt tttcctttag agcttgaaag tattgggcat gactttacag cttccattac
    29521 tttttccaca ttcttatcta ctggtaaata agcaatcatt atactttcat cctctttatt
    29581 tcttctaggt aatggtacga atgaaggttc tccataatct ccatataaac ctaaccttga
    29641 atatatattg tatactattg cttgtctttg accggagtca aataatattc ctgcattagc
    29701 ataatcctta tttttaggaa ttatttgtat tcctacacct ctattaatat atacttctct
    29761 atcgcttttt agacctatat ttgttatacc ttttaaatct cccaccttta gtatagattc
    29821 gtccacaaaa ctaatttcag caggcattac atcagctatg tctccaatac tttttctagt
    29881 aagaagtttt tttaatataa aggctaacac taatccacaa attgaactta ttaccaaact
    29941 catcccttca cttattttcg tttctgcaag tattacataa tacaatccta ttgttaaaaa
    30001 agatgtaact atacacatat aatttcttac ttcataggtt ctagctatct cttctatgaa
    30061 tgcagctcct ctctttacaa gttgaacttc ctctaaattt tgcaatgtat ttcttctatt
    30121 tgctctcact tgtctaaatt gttctgctgc tagagataaa aaagttatag atgtataaga
    30181 acgatttata agagctggaa ccaataatgc tcctaatgaa gaagcgacga atgctaatat
    30241 aagttgagat agtaaaacat ttggttgcgt tggatactgt ttttggtcta aattcaacat
    30301 tataattcta gatgttatac caataataac agcaacaaaa aatactctat ccattaaatc
    30361 actcctaaaa tatttaattt gaaattttac atgtaaatct acaaagtctt tctagaaaaa
    30421 tcactctgct ttccttttga tgtttctatg ataggtgtac ttttcactac atctatcatg
    30481 acatctatat ctttgagtat tggcatatat ggtataacca cagtactttt ttctaaattg
    30541 gtcttagata tagcaacaat atcaacctca tctacatctc tatctatccc catatgaata
    30601 aatagattat gtattatagc ttgtctttgc cctatatcat ttactattcc aaatgcacct
    30661 aaatctcttg gtataacttc tatggccaga cattcatttc tatacttttc tcttgtactt
    30721 tctagaccta tgttagttat tattacacca tttaccatta aaataggtcc ttcaaaactt
    30781 atttttgcag gaactatatc tgctatatct gctatactgt tccttcttaa aaatcttcta
    30841 aaaatcaaac caacaatagc tcctgacaca atagctagga ttgtgcaata aaaaaagcta
    30901 aggccatact ttcttgcaaa aacgatataa actatcgaag ataccagtgc agaaaaaaga
    30961 cttatataac ttctagattc atacgtagat gctatttctt caacgtaagc gtctcctttt
    31021 ggtactaatt cttcattatc gatattcttt agtgttattc tttcttgttc tgcaagacct
    31081 tgaaactgtt gtattgctac tgcaaaaaat gtcaaggctg caaactcctt atctataagt
    31141 gcaggtaatg ctatagcacc taacgaagct gaaagcccag atataattat ctgttctaaa
    31201 taatcttgtg gtcttgatgg gtattgtttg tctgtaactc ttaaaactaa tcctctacat
    31261 aatattccta caactaatgc aactataaaa gagtgtctaa ataaatttgc attcaataaa
    31321 ctttcttcca ttttctcagc tccttctctt atatagtttg tgcaaaaaac aaaaaaaatt
    31381 cccttctatt taaggaaatt tttttgtaat attgtaacta taaattcgct accaatgcca
    31441 agttcacttt ttacttttat acatccattt agtgatttga ctatatgctt tgttatagcc
    31501 aatcctaaac ccgttcctcc tacatcccta cttcttgctt tatctaccct ataaaatcgt
    31561 tcaaatatgc gttctacatc ttcttttggt atccctacac cattgtctat aactttaatt
    31621 acgatttttt cttcatcttt taacactaca acttgtacaa tcccattatc ttgggtatac
    31681 tttatagcat tatcaactag gtttaagaac acttgcttta tatagtcttt attggatagt
    31741 atacttattg atttatcatc acatttatat aaaacttcta tatttttttc actagctgaa
    31801 tgttgagtta tttcatatac ctctttaaat acatcatata ctacaacatt ttccataaga
    31861 agagtatctt tattttctac aaatgataac agcaatatat catcaatcaa cctttttaat
    31921 ctatctgatt cgctttctat tatttctaag aatctatttc tagtattcac atctatattt
    31981 tcatttgact ttagtgtttc aacaaaccca cttatagatg taagtggtgt tttaagttca
    32041 tgacttacat ttgcaacaaa atcacttctc atattttcaa gtttaacttt tttagtaata
    32101 tcttctatat ttactatcga acctattatt acgttgttag tattctgaag ataaactggg
    32161 tctactttta tcttatatac gatatcatct ttagtagtaa tattagcagt ttcattatct
    32221 ttagaaccaa taaattgaag tatttgtttt aatatttctt cttcttttat tacaacatta
    32281 atattatgac cttcaactaa ttgatttggt tcactcttta tcatttcttt tgcttcatca
    32341 tttatcagaa gtatatttcc atttacatct atagccaaaa ttccatgaga tatactcttt
    32401 aatatagacc ttaactgtaa atgtttatac tgtacttctt ctaaggtttt atccatattc
    32461 tttatcatat gattaaaatt tctagctaat tcgccaagtt ctcctttggc tgatatgtta
    32521 agcctagcat gatattcatt attacttact ttttttgaga cgtatataaa ctcttttaaa
    32581 tatttcctta atgttattgt gtatcttaca gctaatataa caatggctat ggacaacata
    32641 acaataaaaa aatatatact atttaaatct tccacaagat aacactccta aaatataacc
    32701 tttttaatct attttgtatc ctacacctct tatagtctct atatattttt ccgatttatc
    32761 ttcatcttct attttttttc ttaaatatct tatatgaaca tctactgttc tagtttctcc
    32821 atagtattca tacccccata ttttatccaa aagataattt cttgaaagta cttttccttt
    32881 attttgaatt aataatttta acaattcaaa ttccttaaga gttaactcta tcttttcaga
    32941 gcctttggta acttcatgtt ttgataaatc tagttttaaa ttacctgtag taagtatatt
    33001 gttttcttca cctaagctag tcaaattata tctccttaaa acagaactaa tcctagctaa
    33061 caactctttt atactaaatg gcttagttat atagtcatca gcacctattt caagcccttc
    33121 aaccttgtca ttttccatat ttttagcagt aagcattata acaggaatat tttttaagtc
    33181 cttatcactt cttatttttc tcaaaacttc tattccactt atatttggta acatccaatc
    33241 taaaagtatt aaatctggtt taatttcctt agcctttata aatccgtcaa atccatcata
    33301 tgaataacta acttcgtaat ttgacacctc caaattgaat tttaatagct caactatatg
    33361 catttcatcg tctataacaa gcactttagt attcattaaa aagctcctct ctattttatc
    33421 tgtagtaccg ttcctattcg ttctgatgcc ttattatgtt ttctataaga gtggaattta
    33481 tctgctctac aactagtgca taaatttaaa tttattatat tttctttttt aactccacta
    33541 catctaagca tatactcatt aattttccat aaatctaaat agtaacttcc ctcttttatt
    33601 atatagaact tttcatcatt atttgtaaga attgtgttaa atttttctac taaatcttca
    33661 gacacttcat aacaacatgg tccaattgat ggcccaatta tacatactaa atcttcagga
    33721 actgttttat agcattcaga cattaatttt attgttttat gacctatatt actaaaagtc
    33781 cctctccaac ctgcatgagc cagtcctatg gctttatttt tagcatctat aattgatata
    33841 ggtacacagt ctgctgtaaa cagtaaaagt ggtacttttt ccaagtttgt tataagtgca
    33901 tctccatctc ttcttttccc tatagtatct ttattgacta tattaactat atctgaatgt
    33961 atttgagaat tactagttag attttcaaat gtcaaatcac attctttaca tacttttaac
    34021 atatcttctc tattttttgc atctatattc ttacatgtta taactagatt tacagaattt
    34081 aatttttcat caagttcttc acaattacca cttaaattaa catactcttt aatatcttgt
    34141 acatttttta tatttatata atctttcatc atttgtctcc tttttctatc agtatacttt
    34201 taagttcatt tacaaaagta tttatatctt taaattgtct atacacagaa gcaaatctta
    34261 cataggcaac ctcatctaac tctttcaatt tatccattac catctcaccg atttttttgg
    34321 tttcaatttc ttctataaaa catttattta tttcattttc aatatgtaca acaatacttt
    34381 caatctcgtc aatagaaaca ggtctttttt cacatgcttt tagcaatcca tattttatct
    34441 tttctctatc aaaatattct ctactattat cctttttaat gaccattaaa ggagttgttt
    34501 ctattttctc atacgtagta aacctctttt tacatgattc acactcacgt cttcttctga
    34561 tagattttaa atctgtatgt ctagaatcta ttacctttga ttctttataa ttacaataag
    34621 gacattgcat ctatattcct cctttgttta actatatctc aaactcatct aagcttaatt
    34681 ctgaacctat atttactatt attgtatcac aacctatttt aagtatatca ttccaaaata
    34741 taacttcttc atcccctcct cttgaaaaaa aacttctact tccatctcca aaaatggtaa
    34801 atgagactac cttaccttcg tgaatatcca tgtctatgtc tattataaac cccatccttt
    34861 ttccattttt tacatttata atttcttttt ccattatatc agaaactcta atcatattgt
    34921 actcaccctt tctttaaaaa taaatatgta tcgaagtaaa aaaaaatgac ctatataggt
    34981 catttaaatt aaaataatgt ttacagtcca tggattactt atacatattt tctcatattt
    35041 tttaaagcat ttttttcaat tcttgatact tgtgcttgag atataccgat ttcatcagca
    35101 acttctatct gagttcttcc tttgtaaaac cttaaatcta ataccaactt ttctctacta
    35161 tttaatttct taatagcttc tttaagtgat atttcttgca accaattttc atcagtatct
    35221 tttttatctt gaacctgatc cataacgaat attgcatctc cattgtcttg atatactggg
    35281 tcaaacagag atattgggtc ttgtatggca tcaagtgcca ttacaactga ttctacttct
    35341 aattctaatt cctttgctat ttctgatact gtaggttctt tagaatttgt tctaattaat
    35401 ctttctctaa cttgtagtgc cttatatgct atatctttta atgacctact tactctaatt
    35461 gggttattgt ctcttagata ccttctaatt tctcctataa tcataggaac agcataagtt
    35521 gaaaacctta cattttggct taaatcaaag ttatctatag cttttataag accaatacaa
    35581 cctatttgaa ataaatcgtc tatattttct cctctgttgt taaacttttt tattacactc
    35641 aagactaatc ttaaattccc tcttacaaac tgttgtctgg cttcttcatc accatttttt
    35701 atttgtaata aaagttcctt catttgctta tttttaagaa ctggaagttc tgatgtatta
    35761 acaccacata tttcaacctt attaacttgc atatattttc agtcctctct caaaagtatt
    35821 catctattga aattatttac atttccaatt tttttatact ttttaagaag aattaattta
    35881 gcattataca aattttttca tttctttttg caaccttgag attattttct tttcaagtct
    35941 agatatgtag gactgtgata ttccaagcat tccagcaact tctttttgtg ttttttcaat
    36001 acctttatca ataagaccaa atcttaattc cataatttgc ttttctctgt cacttaatct
    36061 gtcaagtgcc ataaccaaca aatctctatc tatttcttct tcaattattt tgtatatttc
    36121 atcattttct gtacccaata catcagataa taaaagttcg tttccatcta aatctatatt
    36181 taaaggttca tcaaatgata cttctgtttt ctttttatta ttttttctta agtacattaa
    36241 tatttcattt tctatacatc tcgaagcata agttgccaat tttatatttt tattcagttt
    36301 aaaagtatta actgctttta tcaaacctat agttcctatt gaaatcaaat cttctacatc
    36361 tatacctgta ttttcaaatt ttctcgatat gtagactact aatctaagat ttctttctat
    36421 taaaattgac tttacacttt catctgtttc tagcttttgt aaaagctcca tttcttcttc
    36481 tggtttcaat ggaggaggaa gtatatttgc ccctcccata tagtatattc cctttggctt
    36541 tattattttt aatttaatac ctaacatagt aataaagctt attattcttt ctttcaatcg
    36601 taacattcct tgtccccctt aaattttcac ctaaaatcca ggcaatatat ttggattcat
    36661 tattgctcca tattctctat cattaaaatt tgaaatacca agtattatat ttcctatctt
    36721 ttgctcatct acttctatat agtcagcttt taagcctatt attatgcttg tcttattact
    36781 tccagcatgt ttataaggta ttattctaac tctacctgaa gttttatcat ctaaacttcc
    36841 tataatttct tgtgctttct tcatatctat atcttcataa ttaatattta atagtttttc
    36901 tggaataaat ccttctaaca cactagactt aactattatt acatcacttt gacttattgg
    36961 gtcttttaac aaattaccac tatctactaa agccttacag caaaaacttt tttctagtaa
    37021 gtttatattt atagtctttg taagctcctt aatgtactta agcattttca aatctttata
    37081 aatgtatttt aataattcac aactcacata agtacatgca attaagaaag atatttttaa
    37141 gtgacttatt cctgtaaaat aaattataaa ataagtgctt ccagaaataa aaatgtttac
    37201 tagataaaaa actaacgcta catgaacata atcttttttg ctcttacttc taaatgaaat
    37261 taaagttatt atactcatga ttattatttt aaatggtaca gtaaatagta tattcattga
    37321 tggatacaaa taagcaacag aataggcacc tcctaaacta gcccccaata acttattttt
    37381 tttaggatta tatcttttag taagaataga agtacaactt ataataatat aatttattac
    37441 taggttttct ataacatagt actcaatata caccatgtat ccccccttaa aacctttaaa
    37501 catattataa gatagtatgt acaaaatttt atgtcacttt tgatggtcga atccaaaaca
    37561 aattctattt aaaaagaaca atgatatatg tcttatactt tatgtcgtca aaagatattt
    37621 ttttaatacc aaacaaatag acttttttgt agaatccaat atatctaggt agaagttatt
    37681 cactctactt ttggtatatt tacaaaaaaa gactacataa tttaataata tatcaagtaa
    37741 atcttttact tatataaaat caaattatat agtctgtaaa ttcaaatata aattattgtg
    37801 ctagtaatcc atttgcatat tttgcagatt catttagtgc ttgttctgga gttatgtctc
    37861 cagctatcat catttgaagt tgtttccata aatattcata tatctctact ccatgaggtc
    37921 caactgtcaa tggtctatac atatgtttat ctcttgtcac catttcttca aatcttgggt
    37981 ctccttggta tttttcacct ttagcaactg gagctctagg tatttctttg tggaaagctt
    38041 ccattgattc tttgcttagc atatactcaa gaagtttaaa tccaagttct ttatttttac
    38101 atgctgacat taatgttaaa tcatctactg aagcaaaact tccaactgtc ttatcttcta
    38161 atgatgttac atatccccag tttaaatccg ggaatgattt atcaaaagta cttgtagcag
    38221 cagaggatag cattatagta aatgcagctt ttcctggtcc aaatacactt tcaatcattt
    38281 cattactatc ttttgataaa gcatcttctg gaatatactt tttcatatca tttataaagt
    38341 ttgcagcttt caagccttct gcattattaa atttaactga ttttaaatca tcattatata
    38401 tatctccacc agcttgccat aaatacggat accagttcca gtttaagctt ccaaatactt
    38461 tagaacccca accttgtgca agaccccact ggtcgatttt tccatctcca tcagtatctt
    38521 ttgttgcttt ttcacagatt cttttaaaat catcccatgt ttttggaggt ttttctccta
    38581 atttttctag tatatcttta ttgtagtaca atacgcctgg attagcagct tctataggta
    38641 aaccataaat accacccatc atttttgcat catctaagta tagataatta tctgaagttc
    38701 ctgatttttc taaataaggt gttaaatctt ctaccgcacc catttctata aattgaggaa
    38761 acatctctgc atacatgtat cctatatctg gaccttcacc tgcacttatt gctgttgcat
    38821 atttttctgc gtagttatcc catggtacta tctgaaattc aatttcacag ttattttctt
    38881 tttcaaattt ttcaaatatc ggcttccatg catctgcatc atggtctgtt aatggtgcag
    38941 accatacaac taatttttct ttaccctctt tgcctttaga atcttctttt tttgaacatc
    39001 ctgcaagaat tgatactgat agaatcccta ccataactaa agaaagaacc ttttttagtt
    39061 taatcatgct taatcccctt tcagacttta atttgtcttt ttcctcaaat gtttacattt
    39121 aattcagatt taatattaac aaataagatt actcttgtct aatcataaaa aactatacta
    39181 actcctataa ctatattttt tttctatacc tattagattt aattcatact aaaaacttac
    39241 tttttaaatt ttatcaatat ttaaaaattt ttttaagtct agccacaaac taactatcta
    39301 aattgagcaa aataaaaaga agtctattga cttctttata agcttacata ttatcttctt
    39361 cttcttagga atgtaggtat ctccatgctc tcatcatcat cttcttcaat tatacttgcc
    39421 tttttaggag gctctatttt ttcctcagca attacttctt cttcaatttc ttttacagct
    39481 tgttttactg ttgtatttaa tgaagaacgt atgcttgatt taggtttagt atctaaatct
    39541 aaggcaccat tttgtaatcc ttcaaatcct gtcgcgatta ctgtaatcat tatttcatca
    39601 cctaaatctt cttttataga agctccaaat ataacatttg cttctgggtc acaggattcc
    39661 ataactaaag ttgatgcctc attaacttca aataatccta agttaggacc acctgtaaca
    39721 ttaagaagta ctcctttagc tccttgtatt gatgtttcta gaagtggaga ttgaatagct
    39781 tgtcttgcag catcaattgc tctagtttca ccactagcac tacctatacc catatgagca
    39841 agtcccttgt ctttcattat agtagtaaca tctgcaaagt ccaagtttat taacccttca
    39901 actgctataa ggtctgaaat tgattgtata ccttgtttta atacatcatc tgcaactgca
    39961 aaagcatcta acattgatgt atttttttgt actatctgta aaagtctatc atttggtatc
    40021 gttataagag tatctacttt tgatttaagt tctgctattc caccttcagc gttcttcatt
    40081 cttatcttac cttcaaatgc aaaaggtttg gtaactacac ctactgtaag tatccccatt
    40141 tcttttgcca agcctgcaac cactggagca gctcctgtac cagtaccacc gcccattccg
    40201 gctgttacaa agaccatatc agctccttga agtaacttta ctatttcatc tttactctcc
    40261 tcagcagctc ttttccctac ttctggattt gctcctgctc caagtcctct agttaatttt
    40321 tctcctattt gaactttata ttcagctttt gatgtatata aagcttgttt atctgtattt
    40381 actgatataa attcaacgcc ttttagttga gcttctacca ttctattgac agcattgttt
    40441 ccaccgccac ctacacctat tactttaatt tgtgcacatt cttctaattc tacgtcaaag
    40501 tttagcatca taaaccccct tctctcatga ttcaaagtta tcgtatgtat taattatatc
    40561 attttcttct tttattttgt taacctttaa gtaacatatt ccacaaaaac gtaaattttc
    40621 ctttttaaat ttcttactta tattgtcaaa tattactctt taagtataaa ataatttacg
    40681 tatatattaa atgctcttta atttttacgt tttttgttca taatattact aataatatat
    40741 cgtctaatta tagataagtt attaaacaat ctcataccaa aaactaatac agcaacatag
    40801 taaagagaaa ctcctaactt ttctcctata taagtcaata atatagccaa tatagcatta
    40861 cctatgaaac cagtaataaa aattacatta tcatagttat cttcaaaatt ggattttatc
    40921 gctccaaaaa tagaatccat tattgcaaga atagctactg acatatatat ggaataatcc
    40981 atagaatatg taaatggaat atagtaacct actactacac caaatgctaa tcctcctaaa
    41041 acccataaca taaatcttca cctctttatt ttactttgaa acacaactac attacaatcc
    41101 tttttattaa ttttttttac tttttcttta tttttttgta tttttcgttt tattttgcaa
    41161 ataaatccac tttatttcca ttattttcct gaacttttat attaattcca tatatatcct
    41221 taagagattt agcatatgat tctggagagt ttatagctgc actcaataaa tctatatctc
    41281 ctatagcttt tattataaat ggttgtccat atgtagtatc atttactgta atagtagctc
    41341 ctgaacattt tatcttactt gttgctagta ctctttctcc atttatagaa attgctcttg
    41401 ctccagcctt ttttaaatca tttaaaaccc tcaaaatatc tatatcatgt ataatcaaat
    41461 cgtttggatt ctgtccgtct tttaactctc tctcactatc cctcatagta atagttatgc
    41521 ctggccctgt aacagttgaa taaccactta attctttaac ttcttcaagc tctttttgca
    41581 taagtgtttg gatagattca tttccattct tttttgcttc tttatattca ttcaaagttt
    41641 ttttgttgtt tttaagagtt tcttttaaat tttttatttc tttttttgta ttttctacct
    41701 cactttcaat actttcagtt tgacctaatg ttatgtatac tctatttggg tctagtgatt
    41761 ttatataagt tgatatgaaa acgcctaata tgaatgctcc taatataact attttctttt
    41821 tcatactatt atttcccctc tgctttgttt atatcactat gaccatttat ttctatattg
    41881 tcttctttgg tcacttttac tttggcatta taatatgttt ctatttccca gacaatacca
    41941 tactttatat taagtgccga agccaatgta tcagaatcac caattgcttt tattataatt
    42001 ggttctttta tgacagtgtc attcatataa agcttgtctt cttttaaata catatatgta
    42061 ttagaaacta ttctctggtc gtttatagat atggcacttg cttgtgctga atttaattta
    42121 tttatcattg acaataataa atcataatta tatagtatac tatcattttt atcagagttt
    42181 cctaagtctt ttgtagaatt tatctcaatt tttatgcctg ttccttcaac atctgtatat
    42241 ccagctaatg tttcatattt ttctatttct gacttaagta tgtcttgttc tccttcattg
    42301 tttttgcctc tataagtctc tatatctttc ttcacactat taacttctga ttgtagattc
    42361 tctttttctt tttttaaaga tttaagttct tctgtaagtt gctctcctct tttaaaagtt
    42421 gtcatccctc tattttctat attaataatt ttaagttgta aactaacaag aacacccaat
    42481 acaatacttc ctatgattat acttttattt ataaatcttt ttttcaatat atcacttcct
    42541 tacaattgtc tatccttctg gactatacac tgccaacttt ccatagttaa gatttatagt
    42601 ccctccatta gtatttttat tttgcaggtc aatcagaatt ttacttaccc tagatatatt
    42661 ataatctaag ttatcatcat tagaaagtaa gatttttata ttacttcttg taagcatttc
    42721 tatattgctt tcttttttta agtttacata atttattttt ctataaattc cttcttcttt
    42781 taaataattt aaagttttaa atacattctc cttagtttta taatttttaa attttataga
    42841 tttattatca tcaatgctat aatctacatc tactatcaag tcttttttac tttcattaga
    42901 acctcttagt tcttccaata aatttccttt tttatctata taacaataat tatcttcatc
    42961 ttttaatact gcaaatattt ctttttcttt taaacttata attatcttat tgggaagttt
    43021 tctttttatt tccacatttt ctatatatgg attttttata agtttatttt tcatatcttt
    43081 aaaattgtat gcaaaaatat tttcatttag atttacattt agttctttca ttatatttga
    43141 tttggtcacc ctcttatttc caattacatc tattttcttt acatcaaata aatcactttt
    43201 tacaattata catatacaaa aagataacat aagtataaaa actaaaacta tcatgacatt
    43261 attcgtattt aactttcttc tttttttcaa atataatcaa cccctttggt taataaatac
    43321 acctttagag cttgtttttc ctacaaaaaa attataatat ttaaagtcaa atttttgcat
    43381 tttattacat aacatccaaa taaggtatta aattttataa ataaatcaat atttcatcaa
    43441 aataaacata gcatactaca tactatttat atatttaaat ctgtatacat acaaataaac
    43501 ccctaattta taataattta gaaaatctaa tatattataa attaggggta aatatttact
    43561 tatcttttct ttattcctat taccttagct tgaccttcta tacttggagt cgtttctttt
    43621 tttatttctt ttacttcctt aataactttt tctttttttg tttttttaga agttgacttt
    43681 tgtgttgaat tgtatacttt cattatctca tcatatataa ggtctatggc ttcaggctta
    43741 cctatagttt tacttgcatt tgccatatca accaaaagtt ctctatctcc taaaagttta
    43801 aatactgctg tatttaggct ctctggagtt agattttttt ctaaaatagc aattcctgcc
    43861 ccttgttttt ctatactctt ggcattatac tcttgatgat tttcagcagt ataagcttta
    43921 ggtattatta tagatggctt tccaagagcc gttatctcag ctaatgaaat tgctcctgcg
    43981 ctacctataa ccaaatcact tgctgcaagt gcatttgcca tatcttctaa gtaaggcaca
    44041 actttttggt atggtttaag atttatatcg ctaatacttc ccataaattc atcataatag
    44101 cttttgcctg tagcaaatat aaaagctata tcttcattta ccatattctt tattacaagt
    44161 ctcattgcat catttatttt tcttgaacct ccactaccac catagcaaag taccattctt
    44221 ttttcgtcac ttattgatag attctttctt gcaatatttt ttcttgataa taaaatttct
    44281 tttctaactg gatttcctgt aaaaactaat ttatcttctg ctgcctctgg aaatctttta
    44341 tgcgaatctt caaaacttgt aagtaccttt gtaactgttt tagataaaat cttattagtt
    44401 acaccaggaa aagaattttg ctcatgtatt atggctggta ttttccccat agatgcatta
    44461 aataaaacag gtccactaac atatccacca gttcctataa ctatatctgg tttaaatttc
    44521 ttaacaatct ttcttgattg ctccaaacct ttaaaaagct taaaaactct ttttacatta
    44581 tcgaaatcta tttttcgctt aaaaccttga actgttacag tctttaattc aaatccatac
    44641 ttaggaacta tttctgactc tattcccttt tccgtcccaa caaaaattat ctcagcgtct
    44701 ggatgctcat ctcttatttt gtttgcaata gctatagctg gatacacgtg ccctccagtt
    44761 ccaccgcctg ataataaaac tttcatcaaa tcatcatctc ctgttaatta attttgacat
    44821 gctttgagat gtttaacact attcctacag ctcccataaa gatggtgagt gaagttcctc
    44881 cataacttat aaatggtagt gctacacctg tagctggcat tgatgatgtc gcaactgcta
    44941 tatttaaagc tgcttgtatt ccaatttgtg cacctatacc tataacaacc atacaagcaa
    45001 atacattaga agtcttcaaa gctattctta cacatctgta gactaaaact acaaatagca
    45061 taataactat tatacaacca atcaatccta actcttctcc tattatagca aatataaagt
    45121 cattttgtgg ctctggtata taaaaatact tttgtttact ctttcctaag cctagaccaa
    45181 acaatccacc tgaacctagt gcatatagac cttgtattac ctgatacccc ttaccaagag
    45241 ggtcttgaaa agggtctaaa aatgatgtaa cacgactaag tctatagggt tcagcaatta
    45301 ctaatgcagc aaataaagca gccccactcc ctatcatagc aaatacaatt ttcatatcca
    45361 ttccagcaac aaatatcatt acaaaagtaa ctagaattac agtacctgct gttgacaaat
    45421 ttggctgaag tattatcaac gcaaaaaata tccctggaac tacgagaagt ggtacaacac
    45481 cttttgtcaa agattttatt ttatcataat ttttttcaat taattttgca gttaatatta
    45541 ttgtagcaaa ctttgcgatt tctgcaggtt gaaatgttaa agctcctatt cccaaccatc
    45601 tttttgctcc gtttgcttct atccctaaag gggtcaaaac taacaataat aatactactg
    45661 ctatagctcc aattgctgta gcatttttct tccaaaaact ataatctatt cttgatgtaa
    45721 taatcataac tataaatcca agtacagcat atattacatt cttttttaga aaatagtacg
    45781 catcattgtg tttaaatgaa gattgtataa aacttgcact aaacaccata actattccta
    45841 caaatactaa aagcatagta gtgtaaaaca ctactccatc aaattcagtc ttcaccctta
    45901 tgtcaatttg ttttttcaaa ttttctttag gcatccaact aatctacctc cattcttcca
    45961 tttaagtaag tttagaggta agagattatt ttaaattgtt tacattatct ttgaagtctt
    46021 ttcctcttac ttcaaaactt tcatacatat cccaacttgc acaagctggt gaaagaagca
    46081 ctatatctcc actttttgaa atttcataag aagtcttaac tgcttcttcc atatctttta
    46141 ctatatgtat gtcattaaat cctttatttt tagcacaatt ttctatattt gatgcagttt
    46201 ctcctaataa gaccaatgat ttaacatatg actttgcagt ttcaagaagt tcatcaaaat
    46261 tacttccctt atccatcccc cctgctataa gtattatagg tctatcataa gactgtatag
    46321 cttttatagt ggaatctgga ttagttccct ttgaatcatt tacatatatt acattgtcta
    46381 aatttcttac aaactcttgt ctgtgttcca ctcctttaaa tgtcataagt acatccttta
    46441 taacttctaa atcaattttg cagacatatg ctattgctat agctgccata caattttcta
    46501 agttatgacc tccaggtaaa cttacatcat ctttatttag aaatttgatt ttatcatcta
    46561 tatctattat tatgtcatta ttttctaaat atactcctcc atttactttt ttagttcttg
    46621 aaaagtaaat tactttggca ttgcatttat cagctaaaga ttttactatc tcatcatcat
    46681 agtttaatac acagaaatcc tcaacagttt ggttcataaa tatatttgcc tttgcttcta
    46741 tgtatttttc catagtatgg tgtctattta aatggtcctc tgtaatattt aatattgcgc
    46801 ttactttagg tctaaattca tctatactct caagctgaaa actgcttagt tcagttacta
    46861 acacagactc ttcacttgaa gtttctatag tatctattac aggatttcct atattaccaa
    46921 ctacatatgt atctcgtttt gctcttgaaa aaatttctcc taccaaacta gttgtagttg
    46981 tctttccatt tgtacctgta atccctataa aaattggatt atccgatagt ttaaaagcta
    47041 attctacctc accaattata tacttgcctg aattttttaa tttcaatatg aatggtaggt
    47101 ctagtggtac acctggagac actacaacca tatctatatc atctacatct tctgggtgat
    47161 gtcctaatat gtattttatg tcatttatag atttcaattc atcaagtata tttctcaatt
    47221 tattttcatc ttttatatca tttacgatta tacttgcacc caatttatct aaatgtttaa
    47281 ttgtagatat accagttttt gcaagtccta ctaataaaac tttttttcct tttaagtcca
    47341 tttttcattc cccctagccc taaaatactg ctattatact tatccaagct aaaacaactg
    47401 ttataatcca aaaaataaat acaactcttg tttctggcca accacactgt tcaaagtggt
    47461 ggtgtattgg agccatctta aagaatctct ttcttgttgc cttaaaatat ccaacttgaa
    47521 tcaaaactga tagagcctcc gcaaaataaa ttcctccaat tataggtatt ataagcactg
    47581 agttagttag aactgaaaat gctacaactg cacctcctaa tgccattgaa cctgtgtcgc
    47641 ccataaacac tctagccgga tatgaattaa atcctaagaa acctaaacat gctccaacag
    47701 ttgctgctgc taaaactgcg acttctgtat ttccagcaat agaacttgca aatagcataa
    47761 agaatacaga aactattaat gttactcctg atgctaatcc atctaatcca tcagtcaagt
    47821 ttacagcatt tactattgct acgattataa acatcattat tggtatatat aatattccaa
    47881 catttatcac aaaatctgta aatggtatca taagttgtga agcacttgat gatgatgtat
    47941 actgataaaa agcaacataa aaagataatg caacttgaag gattattttt tgatatgctt
    48001 ttagtccaag tgatcttttt agctttattt ttataaaatc atctaaaaag ccaataaaac
    48061 caaaacctgc aatacatata agacctactg ccatatcatg actaactttt actcttgtaa
    48121 gtccagttat tagaattgca acaatcatta taattccacc catagtaggt gttccgtttt
    48181 tagctagatg tgtttggggt ccatcatctc taactgtctg accaaattta aattttctaa
    48241 gcattggtat aaatattggt cctattataa ttactattaa aaacgcaatc aatgcggtgt
    48301 acgtaagctc tgttattcct aacatcataa aactcctctc ccttgtcaat taactcatta
    48361 gtttaagctt atttatttag atattcgact attttttcta atctcatgcc tcttgagcct
    48421 tttactaaaa tagtgtctcc tgtttttact aattcattta acttgttaaa cacatcttct
    48481 ctattctcaa agtgatatat attggctgaa ttgaatccta attgttttgc ttcttctcca
    48541 ataaacactg aattttcacc tatagttatt ataatatcag tattattcat agatgatttt
    48601 ccaactaatc tatgaccata ctctgaaatt tcacccattt ctagtatatc tccaagtatc
    48661 gctacccttc tacctttata tcttccaagt atctttaaag cagcatccat agaatctgga
    48721 ctagcattat atgaatcatt aattattgtt aatttttcat tttttattat gtctagtctc
    48781 atttttgtcc ctttaaaatt ttttaaacct ttttttatat aatctaaaga aatatttaga
    48841 cacaaaccaa ctaatatagc agacattgca ttatatatat tatgttctcc tattgttggt
    48901 ataaatattt cctctttttt cccatctatt acacatataa atgttaaact atcctcttcc
    48961 atagtataac tttcacaata aacatcattg tttttattaa agccaaatgt ttttaactta
    49021 tatacatgtt ctttttcttt taatgtagat aaaaacttat catctccatt tacaattaaa
    49081 gtatttgttt catcaaagtt tgaagtaatc tccatctttg ctttcaaaat accctcttgt
    49141 gaacctagtt tttctacatg tgataatcct atattcgata tgactgcaat tttaggattt
    49201 actatatcca ctaaatattt tatttcattg aatccagaca tacccatctc tattacagca
    49261 cactcatgtt ctttatttag attaaataaa gtcaatggaa ctccaaaatg attatttaag
    49321 tttttttcat tttttagtat atttaacttt gcagatatgg cagagtaaat catatctcta
    49381 gtagtagttt ttcccacact acctgtaaca cctataaatg gaatatcaaa tttttcttta
    49441 tagtattttg aaatatctcc tagagccaaa gatgtatctt ttacctctat taaatttata
    49501 tccgaacttt ctaattttat tccattactt tcatttttta taaaagtttt acatccacta
    49561 tcatatgcag acttcataaa tgtatgccca tctaaatttt ctccaactat ggctacaaat
    49621 gcattttggg catttgcctc cctgctatct ataactatat cacttacaca gtcattataa
    49681 tctccatata ttaacttccc attagttgca agtactaact cttttattgt taagcactcc
    49741 attattaaac tcctccttta aaaccttagc tatattctct tagcaacaaa aggcaatgta
    49801 taccgttata tatacattgc ctacgataac tcttaacttt ataattatat actatatttc
    49861 tgtttttttg aagatgttta tgggtatatg ttaattatct gttacttttt agttttcaaa
    49921 atatagggtt atacttgagt cttcattaac ttttactcca ggttttggga atatatcctt
    49981 tacctttgta tcttctggaa gttctatatc tgcatctaag tctggtttta acttcgcatc
    50041 ctccaatgcc tttacagcat ctcctatttt tagatttctt acatctggca ctttaacttg
    50101 cttcttttca tactcagctt tttcttcttc tttatatact ggtttaactc ccaaatattt
    50161 taaagagtca ttcattattt ctttcactat tggtcctgct gtagtactac caaatgcact
    50221 tactcctgtt ggttcgtcaa caatagcaag aactactatt tgcgggtcat cacaaggtgc
    50281 tattccaaca aatgaacata tatactttcc tggagcatac ttaccatcta taactttttg
    50341 agctgtacct gttttaccac caagtctata acctggtata tacgctatct ttcctccacc
    50401 ttcagttaca actgattctg caatttctaa cattttttta gatgtttctt tagatataac
    50461 acttcttact ttttttggtt ttactgtttc agttatattt cctttattgt cagtataaga
    50521 ttttaccact cttggttgca ttaaatctcc accattagct attgatgata tagctgttat
    50581 aagttgtatt ggagttactg atatagattg accaaatgat attgtagcca attcaactgg
    50641 tcctacattt ttttcgttat acagtattcc cttagcttct cctggtaaat ctatacctgt
    50701 cttatccata agaccaaatg attctatata atcatacatt tttccaacac ctaatctgct
    50761 tcctaactca acaaatactg ggttgcaaga gttttgtact gcttgtttaa attcttgtgt
    50821 tccatgaggt ctgtaatgtc tccaacattt aatctttctt cctccaacag ttacactacc
    50881 tgtacatgtg aatttttctc catctttaat tactccttct tcaagagcac tggaagatgt
    50941 aataagctta aatgtagaac ctggttcata tgtatcactt actgctggat ttctccacat
    51001 ttgataatag ccttttattt tatctttatc attatatttt tcaagttctt cttgataata
    51061 tggatatatt ggcgtccttg agtcattagg gtcataatct ggttttgatg ctaaagcaag
    51121 tatatcacct gttttagggt tcatagctat tgcagtaacc ttttttgcat tatttaactc
    51181 ataagctttc tgtactgctt tttcagtata gtgttgaatt acttcatcta tagacaacac
    51241 aagaccatta ccttgtacag gctcataata tttttccatt ccttgtggta tttctcttcc
    51301 tgaagcatca gtacttacta taagttttcc aggctttcct tttaactttt tatcatattg
    51361 catttctaca ccagatatac ctgttgcatc tgaagaagta tgtcctaaca catatggtgc
    51421 aaaattccca tatgggtaat atctttgatt atcttctgcc acccatattc cacttaattt
    51481 agcatctctt atttgacttg ccttatcatc atctatccat ctttttactt ttacaagagc
    51541 catatttttt ttgcttatta aagcgtaaat atctttataa tcttcatcca aaatctcagc
    51601 taccttttcg gctgcttctt ttttatcttc tacttccaca ggtttacacc aaacagtata
    51661 cttagttaca cttactgcta actctttcat atttctatca tatatagttc ctcttttggg
    51721 ttctattggt atatctcttg tttgttgttc tagtgctttt gtactcaacc aatttccctt
    51781 cataagttgt agatacccag tcctaataac aaggcaaaaa aacaatgcac atgctagaat
    51841 aagaacgagt accagccttt tcttacttat cctctttact tttctcaaat ctattccccc
    51901 tagttatcca catctatata ctttatttgt ccatcttttg gatagtccat attgagcaat
    51961 tccttagcct ttagctcaat ttcttttatt gatgtttttt cagtttcttt tgcctttagt
    52021 tcctctaata ctatctcttt ttttcttaaa tctttttgta aataatagat atcgtacttc
    52081 atttccgaga tttttgaata accacataga ttcaatatta ttactgaaaa agttgcaatc
    52141 agtaaaatgc atttagctcc tatttttttc ttatttctct ttttataatt attctttttt
    52201 ttatttttat attcatttag attatgtatt ttataatttt ttttcaaatt ctcaccccga
    52261 tagtatatag tttaaatttt cctctatact atatttgtta ttaatcatat ttcttcctct
    52321 aaaatttcat cgctaaatta atacactatt acacaatcta attgtatttt tttctataca
    52381 caacaaaaac ctataaattc acttatactt ttatatgaat ctataggtta gatattaatt
    52441 tctatttatt tttatcactt ctagtatttt tagataattt cttccataaa tataatatgt
    52501 aactaactat ctactctctg attcctaagt tctccattat attacttaat accatctata
    52561 tctatatttt tatatctaaa ttttcattat atatctcaat aacaaagaac atttcttata
    52621 gtgttaattg ttttttaatt gctttaccag tgcaacaaaa tatataatac tttttaatta
    52681 attataataa tcttatatat aagattattc tttagcctta taactactta tattttttct
    52741 gctactctta gcttagcact tcttgaacgt ggatttactt ctatttcttc ctcactaggt
    52801 aatataggtt ttctagttat tattttcacc tcagattctt tatcacattg gcatataggt
    52861 aaatgctgtg gacatataca atctgatgct aaatgcttaa atgcattttt cactattcta
    52921 tcttctaatg aatggaatgt tattatacat attcttccac cttcattcat tatagaagaa
    52981 gcatcattta tcatctttgt aattactcca agttcattat ttacttctat tcgtattgcc
    53041 tgaaaagttc ttttagctgg gtgaggtcca tctattctgg ctttcttagg gatagctttt
    53101 ttaattacat ctactaattc tcctgtagtt tctattggtt tgtttgctct ttcttcaact
    53161 ataaatttag ctatacgctt agcccagtta tcttctccat aatcttttat tatcttagtt
    53221 aattcatctt ctgtataagt atttacaaca tcatatgcag aaaactcaca cctaacatcc
    53281 attctcatat caagtggtgc atcttgcata taagaaaatc ctctatcagc ttcatcaagt
    53341 tgatgagatg aaactcctaa atcagctaat acaccatcaa ttttgtaaac tcctattttt
    53401 tctaattcct cttttatatt ttcaaaattg ctgtgaacaa acttcactct actttcatat
    53461 tcactcaatc tctcttttgc tgtgcttata gcatttttat cttggtcaaa ccctataaat
    53521 aatcccttat ctgacagttt ttttactatt tctttagaat gtcctgctcc tcccatagta
    53581 cagtcaacat acacgccatc tggctttata tttaaattct ctatacactc atttaaaaga
    53641 actgatacat gatgaaattc catagttacc tcccttaaat tcacttttta aactattctt
    53701 cactaatatt atttttattt ttctttgata aataaacatg tgcaattatg ccaccaataa
    53761 ctccaattag acttataact tgtgccattc taagtggtcc taacattaaa ctatctgttc
    53821 ttaatccctc tataaaaaat cttcctattg aatataaagt tatataagta actataactt
    53881 gaccttcata ttttttattt tttctaaata gtaataaaac tataaatatt ccaaaatccc
    53941 atatagactc atataaaaaa gttggatgta cctttactcc atcaaccatt attccccaag
    54001 gtaaattggt agcacctcca tgtgcttctc cattaataaa atttccccat cttccaatag
    54061 cttgtgctaa aggcattcca agtataactg tatcagccat ttttaagaag ttaatatttt
    54121 taatcttagt gtatatataa ccagctaata ttccaccaat taaccctcca tgtatagcaa
    54181 gtccaccacc tctaaaatta aaaatttgag acatattttg agcatagtaa ctccaattaa
    54241 atacaacata atagattctt gcacagatta gtccaactgg aattgctatt atagctatat
    54301 ttaatacatc atcttcttta attcccactc tttttgcttc ttttattgct attaaagttc
    54361 ctaaaatcat tccacatgcc ataagtattc cataccacat gatgtctatg ccaaacagtg
    54421 taaatgctac tctatccata acgtcacctc tttgtattaa ttgaaaatat ttccaaagca
    54481 atatgctata attacaatta attgtaatta tactaaatta tgataacaca ttaatgttaa
    54541 ttctacaaaa aatattaaaa tttattctac aactccctac actatattat atctaattac
    54601 tataaaattt tttaaactaa atctctcatg actaaagtta caagtgtaca tagatatttt
    54661 ttaatcaaaa agattgatgc atatatacat caacctcttc taattcttat ctatagctct
    54721 taaattttta cctcaattta tataaatata ttttataaat ttgttttacc aaaataaaca
    54781 tttaaaaaca taaactaata cttaattagt tattcttatt atgattaagt ttctatttag
    54841 gctctacaat agaaataaca caatactctt ctttaaaatg ctcttttaat ctttcttcaa
    54901 cttcctcaaa agtaatatct ttaataatat ctaaataatc caatagattt atatccttaa
    54961 aaacataaga tataaaacta ttacctataa agtttataga atcaaagcac ttaataaatg
    55021 accctatttt tttcttttta gttctctcaa actcttcttt tgataatcct tctttttttg
    55081 atttttctat ataatcaaga attatttctt ttactttttt aggctcttta gaatctccag
    55141 ctattatgct aaaagcataa tccacttgag acgaaaatcc agctccaaaa ttttcattta
    55201 ttaaaccttg catatacaaa tcttcataaa gcttacttcc ttttttaaat aacatcccta
    55261 caagtatatc agttacaatc tctttacgta aaagttcttt cccttttaaa ccaacattgc
    55321 tatctttaaa acctatgttg aacataggca tagatatagg gaatttttct ataacctctt
    55381 tttcttttac actttctggt tcttctggat aaaatctttc tatactctta gagagtctgt
    55441 ctactttgta attgtttgac tttttagtta catctataac tttttctaca tctaagtcac
    55501 caacaacaaa taaagccata tttcctggat tatagaaagt attataacat ttatatagct
    55561 cttccttagt tatcttatat atactatcaa cagtacctgc tatatcaatt ctagcaggat
    55621 aatttacata cattgccttc aagcaattaa agtatacatt ccaatcaggg tcatcattgt
    55681 acatttttat ctcttgtgct attatacctt tttccttttc aacattttca tctgtaaaat
    55741 aaggtgtttg aacatagtca attagatgtt ctaaactctc ataaaaattt tcagtagcag
    55801 aaaataagta ggctgtcatt gtaaaattag taaatgcatt tgcatttact cctaattttg
    55861 aaaatttatc aaatgcatct cctccatcag gttgctcaaa catcttatgc tctagaaaat
    55921 gtgcaatacc ttcattcact ctaattttct tatcttctcc aattggtaca aattctaagt
    55981 catttgaacc ataattagta gccaaaatag catacttttt cataaaaccc cttttaggca
    56041 taaaatatac atctaatcca ttttgcaatt tctcataata aacttcttct ttcaatatat
    56101 cattaactat tttttccata gacatctcct ccattaattt ctcaagaagt atacagtatc
    56161 aagctctata ttttttatag cttcaactat atcttttttt gtaacctttt ctatactaga
    56221 tattatgtct tgtacttcag aatttgtctt tgccatagac tgagagaaaa caaaatcaga
    56281 cataccacct atattatcag ttatagattt catagaatta actaaagcaa gtttactgtt
    56341 ttctaattct tcatcagata tatcacctgc ttttacactt tctacttgtt tttttatcaa
    56401 ttctacagct ttatcataat ctttagtttc tataccagat gatataaaca tgatactttt
    56461 atacttctct attgaagaga atatatagta acataaactt tctttttctc ttacattaac
    56521 aaataattta gagtgtggac caccacctaa gacattgctc ccaactacta aagcataata
    56581 tttatctatg tctgcataat ccacatttgc tctataaccc ataactaact taccttgagt
    56641 tatttccatt tgttcatcta taacttttac ctcatccaca ttttttataa aatctgctct
    56701 tggtatattt ataatttctt ctctttcaaa ttttagattt ttacttatta tatctacaac
    56761 tttatcttca tcaaaattac cttctacaac tatatctata ggtgatgttt taagtatgtt
    56821 tttataatgt tcatataact ctcttgaagt tatactatct atttcatctt cataaccaaa
    56881 ttcacttaca ctatatctct ctcctttaca catagcctca atacatttat cttgcgcata
    56941 ttccttttta tcatttatta ttgattgtat cttttctctt aaattctctt tttcaatatt
    57001 taaatattct tctttaaacc caccatctac aacaagcgta ttatttatta cttcattaaa
    57061 aaattctact acttcttcaa atatactctc atctaaatac ttttcactta tgttaattac
    57121 tttaaaactc aatacttgac gttctccacg ttttacagca tcagctccca tagatgaacc
    57181 atataaatca tctaatttat tggatattgc tctagcacta ggatatttag cgcttccact
    57241 tgctattatt gatggtatta gagcattttt tgtagtttct ttttcatcca atagtctctg
    57301 aacatataaa cttataagat tcgatttaaa cttttctgtt tttatcaaag taagattaat
    57361 attattacct aaatttatta tttttttagt attaaccata tgtacctcct atttttattt
    57421 aaacatcaat tttaaaattg aaatttattt attttttaat tacatatact caca
//
LOCUS       DAMJYR010000010        56229 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.010, whole genome shotgun sequence.
ACCESSION   DAMJYR010000010 DAMJYR010000000
VERSION     DAMJYR010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 56229)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 56229)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..56229
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.010"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            171..692
                     /locus_tag="PC471_000612"
     CDS             171..692
                     /locus_tag="PC471_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF2787753.1"
                     /translation="MDIAKLILNPARLRILQYIRLYGSVRTSDIVKYLNDIPRATVYH
                     HVKILEENNMIEVIKENRVRGTIEKVYALKEYTTSMEGETFVALSTAFYVGLMQEMNE
                     YFSRKEQDHKKDNVFFSSALLYMSDNEYENLLKSIADLLKPYIEQKPKSNLKLRKLSI
                     ISSPPVADNDKTK"
     gene            726..1502
                     /locus_tag="PC471_000613"
     CDS             726..1502
                     /locus_tag="PC471_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDF2787754.1"
                     /translation="MKFHEFGNKNRPHIMLIHGGGNAWWNYLRQAEVLSNKYHVILPT
                     LDGHGEEYETTYISTEDTADKLISYIDENCSGHLFALCGVSLGGQIVMELLSRKPNIT
                     KKAIIDGSICYPRPLMARFCIAGIRFFGGLLFSEKACRFQIAMMPKLLPENMQYPDKI
                     KAYYMTDMPHVRKETFYNMYRTYMMNYTLKENVKKTTAQVMYWYGEKEMKCVKKSAKL
                     FQSYVPSCEIYEAKSYNHGYLSLYLPNEWLEIAEPFFQKE"
     gene            1844..2797
                     /locus_tag="PC471_000614"
     CDS             1844..2797
                     /locus_tag="PC471_000614"
                     /EC_number="3.4.24.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPR endopeptidase"
                     /protein_id="HDF2787755.1"
                     /translation="MISVRTDLALEASEMCEKSQEGSSIPGVKIETKELENCIVTKVE
                     VIDRQGAEIMNKDIGKYITLESNLMKFDDDESREEMISYLKEELVDIFGQDKNKKTLV
                     IGLGNRNITSDALGPKSVSKTLVTRHLFKNYNKDYDDDFTEVSALSPGVMGVTGIETS
                     EIVKSLVEKVKPDRVVAIDALASRKMERVNSTIQISTAGISPGGGVGNTRKSLTKETL
                     GVDVIAIGVPTVVDAATLTIDVLDMAIDNLIAQSEETESFYEMLKKLKEEEKYHLIKD
                     SLDPYDKNLIVTPKDIDDTIENLSIIISEGLNRSLHPGRLV"
     gene            2815..3834
                     /locus_tag="PC471_000615"
     CDS             2815..3834
                     /locus_tag="PC471_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein P"
                     /protein_id="HDF2787756.1"
                     /translation="MFKKCIKVVTLTFILVCILPGKSLALNQDDFLKFLVNSSYPEAK
                     VEGNDTENKKNNKNKETSKENKEESKEGNTKSKDASKVDNKKESEKEYIKLYVGKENV
                     PDIESKNSDTTETNTTSSSDYKDDLRVTKENPRILIYHTHGCETYSNSPDGNYHSRDK
                     KNSVMEVGSALTSALDSKGWGVVHTTKYHDYPSYNNSYASSLKTIQSILPKYNSVDIA
                     IDLHRDARDLTNPATKEKDHLKYTTMINGERVSKFFFVVGGKNTNRKQLRALAEDITA
                     FAEKKYPGLVSPIVEKDYARFNQFAVKNHMLVEIGNNATSVEESKATTKYLAEILDEY
                     FKQKN"
     gene            3865..4116
                     /locus_tag="PC471_000616"
     CDS             3865..4116
                     /locus_tag="PC471_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2787757.1"
                     /translation="MSRLEKNIESKSKLRKYKYIMRILFLITMIICTSVCVFIVDSNA
                     KSMLGMKQYDFPSIKFDITKFDFDIPNFDIHEIIKKFNK"
     gene            4398..6203
                     /gene="lepA"
                     /locus_tag="PC471_000617"
     CDS             4398..6203
                     /gene="lepA"
                     /locus_tag="PC471_000617"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HDF2787758.1"
                     /translation="MDNKQSRTRNFSIIAHIDHGKSTLADRLIQQTGLVSERDMKSQL
                     LDNMDLERERGITIKLQNIRLMYKAKDGNEYYLNLIDTPGHVDFNYEVSRSLAACEGA
                     LLVVDAAQGVEAQTLANVYLAIDQDLEILPIINKIDLPSARPEEVKNEIEDLIGLDSS
                     EAPLISAKTGLNIEDVLEDIVKNVPPPKGDNEAPLKALIFDSYYDAYKGVVAYVRVFE
                     GTVKKGMTIKMMNTNKKFEVTEVGVMAPGQTELSELSAGDVGYIAASIKDIRSCRVGD
                     TITDSNNPTEEPLPGYKKATPMVYCGIYPGEGEKYENVRDALEKLQVNDAALEYEAET
                     SAALGFGFRCGFLGLLHMEIMQERLEREFNLDIITTAPSVIYRVTKMDGEVVMIQNPA
                     NLPEPSEIKMIEEPIVKGDIIVPKDYVGVVMELCQERRGNMLNMEYIDDRRVMLHYDL
                     PLNEVVYDFFDALKSRTRGYGSLDYEVKGYVASTLVKLDILINKEQVDALSFIVHETR
                     AFPRGKAMCEKLKGEIPRHQFAIPIQAAVGNKVIARETISALRKDVLAKCYGGDISRK
                     KKLLEKQKEGKKRMRQIGSVEVPQKAFMSVLKLDE"
     gene            6352..7689
                     /locus_tag="PC471_000618"
     CDS             6352..7689
                     /locus_tag="PC471_000618"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HDF2787759.1"
                     /translation="MINIEIPKKVDYIIKELEKNGYEAYIVGGCVRDCLLERVPNDWD
                     ITTSARPEVVVELFEKTIPTGIQHGTVTVMIEHEPFEVTTYRIDGNYSDGRHPDSIEF
                     TNNIVKDLSRRDFTINSIAYNSKTGLVDPFNGYEDIQNKYIRCVGNPVDRFEEDALRM
                     LRAVRFSAQLNFKIAEGTKQSIHKKADLIKNVSIERIQTEFNKILVSDSSKLNLLKST
                     GLLKFIIPEICELEDVTQHNPYHIYDVQKHTLIATEVIEDELYLKLTMLFHDLGKKVT
                     KTTDKNGVDHFYTHSRESVKIAKKILKRLKYDNYTINKVLILIQYHDYRIEPKRKIIK
                     KLLNKLEDVELFEDLIKVNWADTLAKNPKYAKQKILNLIECEKEFKHIINQKECFNLK
                     DLAINGKDLISIGIKPGKDIGYILNKMLEIVINNPELNEKEILKEKALNIYTF"
     gene            7922..9235
                     /locus_tag="PC471_000619"
     CDS             7922..9235
                     /locus_tag="PC471_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDF2787760.1"
                     /translation="MHTQLQKTIGLSAALSTVVGMVIGSGVFFKPQAIYTTTNGAPGL
                     GIIAWLLGGFITITAGLTATEISAAIPKTGGMMIYIEEIYGEKLGFLTGWMQTVLFFP
                     GTSAALGVIFAQQASELLGMSPNNMANVLPIAIGVILFLALLNIIGSSLGGKVQTVAT
                     IGKMIPLILIIVFGFIKGQSSEVLNPFVGDGVNASNALGQVLIATLFAYDGWINVGAI
                     SGEMKSPEKDLPRAIVGGLSLVMAVYIIINIAYLWVVPASELATVTSPATLVATRLFG
                     NIGGKVITVGILISVFGTLNGYLLTGSRIPYTLAEMGTLPASKTLLKVNSGGSPVNSI
                     LLITVLACVYALSGQFNLLTDLTIFSIWVFYVLTFIGVMRLRRQKPDLHRPYKVPLYP
                     IIPIIAILGGLFVIINQILTSTVISLGGIFITLLGLPVYYYMKKR"
     gene            9407..10582
                     /locus_tag="PC471_000620"
     CDS             9407..10582
                     /locus_tag="PC471_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HDF2787761.1"
                     /translation="MLGLYVHIPFCVKKCKYCDFNSYKMDIDSKKRYIEDLKIEMELY
                     SNKLYKDNKYKNKECCSLNKNDKITSIFVGGGTPSILTSDEIREVFISIKEMFDIDEN
                     AEITIECNPGTLTLEKLKTMKEIGINRLSIGLQAIQEKHLNFIGRIHTYEEFEKNYKD
                     ALSVGFKNINIDLMYSLPNQTLCDWKETLEKVVHLNPTHISAYSLILEEGTELYNMYE
                     SNKFELIDENVDIEMYEYTINYLKSKGYNQYEISNYSKEGYNCEHNILYWECEHYIGI
                     GAGASGYINENRYNNVESLEDYHLSLVKREKPIQENEILSEKDMIEEKIFMGLRMNKG
                     IKFEDFKKKFGIDFREKYNKQIEMLLARKLINQSFEGIQLTQKGREISNSVFIEFME"
     gene            10685..11704
                     /gene="hrcA"
                     /locus_tag="PC471_000621"
     CDS             10685..11704
                     /gene="hrcA"
                     /locus_tag="PC471_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcription repressor HrcA"
                     /protein_id="HDF2787762.1"
                     /translation="MELNERKLNILKAIVKDYIETAEAIGSRTISKRHDLGVSAATIR
                     NEMADLEELGYLIQPHTSAGRVPSEKGYKLYVNSLMSKSELDDNDKILIEQCMNHNIN
                     HIKELIHETSKLLSQLTNYTTVAVTKSLINQSVIKHIQLVAMNDNNILLIVVTDKGDL
                     KKANLTTNVYLDQSKLNLISDNLTRKLLGKSITDLDDNLIAFIKYEISEYSGLIDELL
                     NALNSNMKEEDFSLSLNGATNIFSYPEFNDVLKAKSFLNMLEKKETIADIIKSKGIQK
                     DNLNIIIGSDNDCELAQDCSIVTATYNVDRDLVGRISFIGPTRMDYARIYSIINYMSL
                     LINRK"
     gene            11734..12354
                     /gene="grpE"
                     /locus_tag="PC471_000622"
     CDS             11734..12354
                     /gene="grpE"
                     /locus_tag="PC471_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HDF2787763.1"
                     /translation="MDKKNEQQEVREENDTSINQESETQVELEEEVVNEECETSSEKT
                     DEKEVDDENVTDINSKLAEKKLQDELDELNDKYQRLQAEYANYRRRTQQEKETIGVFA
                     NEKIITELIPVIDSMERALDACEDKEDTMYKGISLVHKQLIDTLVKFGVEEIEAESKE
                     FDPNLHLAVMQESVDGVEANQIVMVLQKGYKLGTKVVRPSMVKVSC"
     gene            12446..14293
                     /gene="dnaK"
                     /locus_tag="PC471_000623"
     CDS             12446..14293
                     /gene="dnaK"
                     /locus_tag="PC471_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HDF2787764.1"
                     /translation="MGKIIGIDLGTTNSCVAVLEGGEAQIIANSEGMRTTPSVVAFTK
                     DGERIVGEPAKRQAVTNADKTITSIKTHMGTDYKVNIDGKSYTPQEISAIILQKLKSD
                     AESYLGQTVTEAVITVPAYFTDAQRQATKDAGRIAGLDVKRIINEPTAAALAYGMDKL
                     DQEKKILVFDLGGGTFDVSILEIGDGTFEVLATAGNNRLGGDDFDQIVIDYLAEEFKK
                     AEGVDLRNDKMALQRLKEAAEKAKKELSSTMSSNINLPFITATAEGPKHLNIDLSRAK
                     FEELTRGLVEKTMEPTKTALQDAGLSTGDIDDVLLVGGSTRIPAVQEAVKKFIGKEPH
                     KGINPDECVAAGASIQAGVLAGDVKDLLLLDVTPLSLGIETMGNVMTKIIERNTTIPT
                     KKSQIFSTAADNQTAVDIHVLQGERSMAYDNTTLGRFQLTDIPPAQRGIPQIEVTFDI
                     DANGIVNVSAKDLGTGKEQKITITSNTNLSEAEIEQKIKEAEMNAEADKQKKEKIEAF
                     NQAESTIYQTEKTLNELGDKISSGEKEDIEKAIADLKAVKDNQDATAEELKKATDEVM
                     TKFQKVSQEMYQKAAQEQQAAQGAEQAQDNGPKDDNVVDADFKEVDEDK"
     gene            14504..15640
                     /gene="dnaJ"
                     /locus_tag="PC471_000624"
     CDS             14504..15640
                     /gene="dnaJ"
                     /locus_tag="PC471_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HDF2787765.1"
                     /translation="MSTKRDYYEVLGISKGAEAQEIKKAYRKLAMKYHPDRNPGDKEA
                     EEKFKEINEAYEVLSDDTKRKTYDQFGHDGLNGQGGFGGQGFGGFEDMFGDIFGDMFG
                     GSFGGGRARRRGPQRGADIRQSVTISFEEAAFGKKMSIKVNRSEECEECNGTGAKPGT
                     SKKTCSTCNGTGQVRTVQRTPFGNIASSRPCSACNGTGEVIESPCSKCHGTGNTRKVK
                     TIEVDIPAGIDDGQMIKLSGQGEVGEKGAPRGDLYIVVNVKSHPLFTRDGNDIYFEMP
                     ITFVQATLGDEIEVPTLDGKVKYSVPEGTQTGTVFRLKEKGIPRIRGNSRGDQYVKVV
                     VEIPKKLNDKQKELLREFAKECGSNVHEKKKTFGQKIEDMFKKK"
     gene            16606..17541
                     /locus_tag="PC471_000625"
     CDS             16606..17541
                     /locus_tag="PC471_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HDF2787766.1"
                     /translation="MYYIGVDIGGTGIQAGVVDNYGKIIFRSECKTVIEKGFEGILND
                     IKIMIYKLLEDNKLTMSDIKSIGFGVPGFINKEGLVTCVNLKWNKKAFNKELKRRFPD
                     VEIHGENDATVAALGEAKFGSMKGANVGVLYTLGTGVGGGIVINQKVFSGAHGLGSEI
                     GHQIIGENYFNCNCGNNGCVETFCSATAIIKYSQKLIEEGEKSRILDLAEGNLENVNA
                     KMVFDAYRENDLVAIKVINRFKEYLAKTFANTINSLDPEIISIGGGISKSSDIILDGI
                     EDLVRKFVLYKTEDIATITCATLGSDAGIIGAAFL"
     gene            17577..18950
                     /locus_tag="PC471_000626"
     CDS             17577..18950
                     /locus_tag="PC471_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDF2787767.1"
                     /translation="MGKTNARRTLFLIAIGSGTMLNPLNSSMISLALHSIQNDFHISF
                     TTVSWIVSAFYLASAIAQPVSGKIGDLIGRKVLFLSGLMLVILSASIVPLVQSFFILI
                     FIRVIQAIGTSTLYPSGVALIQNNIKERQSSALAVLTIFASTTAGLGPTLGGLLLDLG
                     GWHAIFLVNIPVVLVSICLGYFLFPKEVKEKKSIKETLKNVLSRLDIIGILLFSIAMI
                     FILLFLLSMKESFNLEQLVFGIILMCLFIWHELRTKLPFIDIKLFVSNPKLSKVYLQF
                     IILNFFNYILFFGLPSYFQDALHYSAKSTGLFMLFMSGIGIFISPLTGKWIDKSGTRF
                     PLVTSSIFMFISAVLLSVVFIHIPVIGKGIILSLAGISYGVGNVALQSSMFEESPRDS
                     IGTASGLFQTSRYLGSILATVLLGMVFEVNITSEQFQILGYVMVVLGTISFLLNFILK
                     KELRKVE"
     gene            complement(19138..19905)
                     /locus_tag="PC471_000627"
     CDS             complement(19138..19905)
                     /locus_tag="PC471_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF2787768.1"
                     /translation="MEELKDFLERQLNKKINIYPYENQKLDASSHLVTFNNAIYVIDF
                     LDKNNLDNLKGNFYLIYQSHIDFEYLKNIFYNLFEDINIIQHNGFFIVNSKYNLDINV
                     TTQNIIETETYQSTYIFYLGELDSKADFDFRLQLCSDLLPHIIKDNAENKFLNLFDFI
                     RYKTLDLINEDNILNKLIDFNKIKSIDEELLYTGIKFINNDLNISKTSTSMFLHRNTL
                     VYRLEKINEILGFDLKNFENAMIFYLSVKSYFLYKKI"
     gene            complement(19935..21044)
                     /gene="ugpC"
                     /locus_tag="PC471_000628"
     CDS             complement(19935..21044)
                     /gene="ugpC"
                     /locus_tag="PC471_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003242648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HDF2787769.1"
                     /translation="MSEVILKNISKLYSNGFNAVKNINIDIKDKEFIVLVGPSGCGKS
                     TTLRMIAGLEEISEGELYIGDKLVNNIEPKDRDIAMVFQNYALYPHLSVYENMAFALK
                     LRNLPKDEIDKKVKEAAKILDLLPLLNKKPKTLSGGQRQRVALGRAIVRNPKVFLMDE
                     PLSNLDAKLRTAMRTEITKLHQQLGTTFIYVTHDQVEAMTMADRIVVMKDGVVQQIAT
                     PQDVYDYPANIFVAGFIGAPQMNFIDVILIEENDEIYAQNEHIKLKLNKEKHYDLIKD
                     NYINNEVVIGIRPEDIHVEDTFIKSNPDTCFKSRIEIKELMGAETYAHLKLGENTITI
                     RFDSKNRINVGDDLTLSVDNSRVHIFDKETTLAIR"
     gene            21302..22039
                     /gene="cas6"
                     /locus_tag="PC471_000629"
     CDS             21302..22039
                     /gene="cas6"
                     /locus_tag="PC471_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endoribonuclease Cas6"
                     /protein_id="HDF2787770.1"
                     /translation="MSRISIFMESCNGKFPNENSMLSVSFIKNILNAENKDFAKNIFN
                     YGEERKNNKQIKDINTAIYIPNLIRSKNELLVDGDIIFNISFYNYSMFSKLYNGILKV
                     KELEYKGYRFKIKNIKIHKEHEIKENGVIFKTMSPIIVKNKEGKYLDVEDSNYIECLN
                     YIANLTLESIRGSGLRKPLEFIPINFKKRVLKEKIRGFKVREYYYINAYAGTFFLKGD
                     MEDLNALYKMGIGYRRTENAGMVDILK"
     gene            22044..23519
                     /locus_tag="PC471_000630"
     CDS             22044..23519
                     /locus_tag="PC471_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2787771.1"
                     /translation="MREIKAKFYRSGDIVRDIGIVYFYELLMDLKNELEKNDYKLKFT
                     CELNRNYLSLESAEYIDPKYISDYILENRLFKVFKNGKKETLEKIFSNIDNLTYVNFI
                     EELDKSSATAKQKEDIKKDFKNRRFPYVRNSGKYGLNTSLENMETNVKRIVDTVIKSN
                     IEIDEIDKLDLTQYEEKDSVCNVCNINKTTKLDIDLRERKDSKYNFLFRGAEKSGFKR
                     SGQVESNICFECEFFNLMCLLYINLKRPMVFAYTNDLRELAFLNHKIMLKRKMYSDKS
                     FYKKLLHEKISSIRLYSFDIDTNKGIILKFDSIIEYKELLKKIELIDIIDNYNFSREP
                     GNTRNLGKRMIDNGNLSNLKELLLDNISVLKQSTGTSREMDFVSSSYNIGLYIKLCWI
                     VDGGEEYKMKNYMESNLIYSRVGKDLFNKMTDESRRKNFSMKVIQLLKSNDRTQLFQT
                     IMHVLVSNGILIGEGFVEGLMQSSELELNTNVGLFIQEIMK"
     gene            23539..24552
                     /locus_tag="PC471_000631"
     CDS             23539..24552
                     /locus_tag="PC471_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DevR family CRISPR-associated autoregulator"
                     /protein_id="HDF2787772.1"
                     /translation="MNEKRVKGVTLTILTESPVPLSYDQGYGNYTPIKKEQYREKIHA
                     KTSIATITYDLRRMLHQEYGWNLSNIVFGKKGNIYPSIKKNVGCVDENGLETDVFGYL
                     IPLKDKGSISKASPLRIIPFRSLNPYKGSTQLITNRGFLSSEFGRKYYDEKEENEVPR
                     DENFPTSQALAIEETLSDYYVYTITLELDRIGVVEVEDGKLLLPEERKFMSKELREKA
                     VKDILDAITVFTRNIKHSSVLLKPLAVIGGAFDKVVPFFWDDVDYNADSGEINLEGVI
                     ETIESYSLKESNTILAINDRLKISNKKELNKFNLSKYPVKEIKNLADRLEIGEDNMWY
                     LKE"
     gene            24568..25365
                     /gene="cas5"
                     /locus_tag="PC471_000632"
     CDS             24568..25365
                     /gene="cas5"
                     /locus_tag="PC471_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated protein Cas5"
                     /protein_id="HDF2787773.1"
                     /translation="MKVLQCKLKQPTAHYRDPKVFQNEYISTLNLPSKTTIMGMITYL
                     CDRRLNSDIDIGIIGTHHHRELEFSRGENIDFWNEYIKMGKGKDKEKFLLKGNYYDYY
                     KEHKAQNSILNYEVLKEVELTIFISCKDDEELEFIRKKLESPCKYANLGRKEDFVIPS
                     EKGCFVKEVKLKEVIPMNTRDAIKENIKLKNTYVRVDLRDKENVETIINQGVLIALPH
                     KYKDLEANRDDRVYEFCHYIYVDNDGIYPKNIKVNVCIDTKEVFTWL"
     gene            25392..27800
                     /locus_tag="PC471_000633"
     CDS             25392..27800
                     /locus_tag="PC471_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated helicase/endonuclease Cas3"
                     /protein_id="HDF2787774.1"
                     /translation="MKNIMQKEILAKSYNLRTNQQEQTLVEHIKDLFEVLESILELNL
                     YSDKDVEILKICCALHDLGKINSIMQQKMEINNKISYSCSEEERKKLESDKKSLKKIA
                     RHNIFSGAFLKDILEKMNLSEEDKLYIYKSIMLHHGNYEDYMRLSISKVQEEIYEYIE
                     KGILEKEEFNLKDIESYINSILNVDFKFGEDVLDYDYIDKLSESMFIDSDYNNGQIDD
                     SVLNYRKFKYILYKGMLNLIDHSASSRQKGIKFYNDFTDEEIDNMILNEIYKSQGNLE
                     KNNIEFNTIQKRLRELSGRNVLTVAFTGSGKTAADYRKTFKRKFFLVPNKISAESFYR
                     KNIFQNKNDLDTRSSNDYIGLLHGDINLYSENEDNGEHDFVLTLRDIDLSINFCKPCV
                     IATVDQLLLSMFKFPGYEKIFAAVKNASITVDEVHLLEPKMFLILIYFMQFACKYLDV
                     DFHLMTATLPKSYKEQMINKGIIFQEESNENVTDKGEIVFIESNKEEEICEGKDVKVS
                     FIKEKEIKSIVEDALENKQKILIIKNTIDSVNRTYEFLKENLSDKYGGVDIDVLHSRF
                     KFKDKKEKYSKILNGEGDIWISTQSVEISLDLDFNIIISDLATMDSLIQRMGRCNRNN
                     KYEYGNFYILPSEDKIYDNKLKSTTKNILKDILKTNSIFTMGIRKTILDDYYDNSVVK
                     KYFEENFISCDKEIKNIYGVNKEIFDGLDLIFNFEPYKNIVDSKKEAAKIFRDVDVSY
                     KIILEEDFYKEDRELQQDSIQVSGFIFNRLYYYGLISKVEGYMVLKSSSKFEYNSTVG
                     LILK"
     gene            28241..29188
                     /gene="prmA"
                     /locus_tag="PC471_000634"
     CDS             28241..29188
                     /gene="prmA"
                     /locus_tag="PC471_000634"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HDF2787775.1"
                     /translation="MNNWIEVTIKTTTEAVEPITNILYEQGAGGAVIEDPKDFLFQKK
                     NELDWDYVEEEVFKKNEEDDVLIKTYVSEEKNVMEFVEIIKQKVLGLKDFGIDIGEGS
                     VSLDQVNEADWANAWKAYYKPTKVGQRVVVKPTWEDYAMQEGDLIIELDPGMAFGTGT
                     HETTSMCIRELEKYVNKDSKVFDIGCGSGILAIAAAKLGAKEVVAVDLDEVAVKVAKE
                     NVLENKVEKSVSVMHGNLTDVIKDKADVIVANIIADIIKILAKDVQNFMKEDAIFISS
                     GIILDKVEEVKESLIENGFEIVEVQKLGEWSAIVSKLKK"
     gene            29207..29965
                     /locus_tag="PC471_000635"
     CDS             29207..29965
                     /locus_tag="PC471_000635"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HDF2787776.1"
                     /translation="MDRFFVEKNNINLQDKTCTIEGEDVKHISKVLRCKLGEKLEICD
                     KNNNEYICEIMNIDKSIVNLEILEKVDINRESELKVRLYQGLPKAPKMEIILQKLTEV
                     GVEEIILVQTKRSVVKVDDKKEDKKFERWERIIYEAAKQSKRGKIPKLRGVLSFKEAL
                     EDMKKNSVNICPYENERTVSIKHALKKCDSNIDSVGIFIGPEGGFSEEEIEQIQKNNC
                     NVVSLGPRILRTETASVVASTIALYELSDLGGEK"
     gene            29967..31265
                     /gene="mtaB"
                     /locus_tag="PC471_000636"
     CDS             29967..31265
                     /gene="mtaB"
                     /locus_tag="PC471_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HDF2787777.1"
                     /translation="MKKVAFYTLGCKVNQYETEAMLELFEKDGYEQVNSEEYADVYVI
                     NTCTVTHMSDRKSRQYIRRVKKKNPDAIIAVVGCYSQVSPEEILDIEEVNLVMGTNDR
                     RKIVEEIKKINSSKKVSTVDDIMKVKAFEEIEISQTNGKTRAFMKIQDGCDRFCTYCI
                     IPYARGRVRSRDIDSIVDEVKKLANNGYKEVVLTGIHVASYGKDLKDRDIKLLDVIKQ
                     INQIEKIERIRLSSVEPILFTDEFVNEVLKMDKVCPHYHLSLQSGCDETLKRMNRRYT
                     TLEYKTIVDRLRSKMPDVAITTDVIVGFPGETNEEFKKTYEFLKEIELSQMHIFKYSP
                     RKGTPAATMENQVDPQMKHFRSEQLLNLSKVNFNKFATKFIGRELDVLFEQNIEGNKY
                     EGLTSNYIRVVVESDKNIQGQILKVKINDVKDEYVEGILL"
     gene            31308..31658
                     /locus_tag="PC471_000637"
     CDS             31308..31658
                     /locus_tag="PC471_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HDF2787778.1"
                     /translation="MDCIFCKIANGEIPSTKVYEDDRVLAFNDLNPVAPYHILVVPKK
                     HYDSLIDIPDKEMDIVSHIHVVINKIAKEKGFDQTGFRVINNCGSDGGQEVKHLHYHI
                     LAGKKLPNYEAGQN"
     gene            31821..32000
                     /locus_tag="PC471_000638"
     CDS             31821..32000
                     /locus_tag="PC471_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HDF2787779.1"
                     /translation="MSEVRVRENETLDSALRRFKRQCAMSGIMSEVRKREHYDKPSVK
                     RKKKAEAARRKNAKK"
     gene            32030..32473
                     /locus_tag="PC471_000639"
     CDS             32030..32473
                     /locus_tag="PC471_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HDF2787780.1"
                     /translation="MSLKQKLQEDLKSSMKNKDTVRKSVVTLIRASIKQYEVDNRVEL
                     DEDGIIDVIAKQLKQRRDALVEFEKAGREDLIKETEGEIEVLKEYLPQQLSEEELEEI
                     VKSTISEVGATSMKDMGKIMSVIQPKVKGRADGKLINKLVKQNLQ"
     gene            32584..33081
                     /locus_tag="PC471_000640"
     CDS             32584..33081
                     /locus_tag="PC471_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HDF2787781.1"
                     /translation="MIISVKEIRNFLINIFIPLIIGYLSSILTMIISGTDISTYYLQL
                     TKPSFAPPAFIFPIVWTILYILMGISSYLILRKGYNLPKVRDAIFYYGLQLVLNFIWS
                     ILFFGFGLRFTALIDIIIMILVSIIMISKFSKIDKRSGYINLIYLMWLVYAGFLNYFI
                     WFINK"
     gene            33274..33837
                     /gene="raiA"
                     /locus_tag="PC471_000641"
     CDS             33274..33837
                     /gene="raiA"
                     /locus_tag="PC471_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HDF2787782.1"
                     /translation="MQIIVSGRQMKLTDGIKGYVDGKLSRLEKYLDPESEVKVTVSAK
                     KDRQKVEVTIIPINGQIIRAEDVEDDLYAAIDIVCDKLSRQVVKYKTKVKDKVQNNKS
                     IRFENLDFIDNSSEFDDYDYDDEEDENIVIERRKKFNVKPMSSEEAILQMELVGHNFY
                     MFRNQDNFEINIVYKRKAGGYGLIEQD"
     gene            34050..34286
                     /locus_tag="PC471_000642"
     CDS             34050..34286
                     /locus_tag="PC471_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YabP/YqfC family sporulation protein"
                     /protein_id="HDF2787783.1"
                     /translation="MLEISTDLQVNQPVITVTSNTFISIENYLSILEYEVDLIRIKTK
                     VKTIKISGDKLSLKYITDSEIGIKGIIYNVEYVD"
     gene            34299..35642
                     /gene="yqfD"
                     /locus_tag="PC471_000643"
     CDS             34299..35642
                     /gene="yqfD"
                     /locus_tag="PC471_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein YqfD"
                     /protein_id="HDF2787784.1"
                     /translation="MISFIRGYYVIVVEGVGLEQFLNHLIRNGISVYNVTRIKNTKME
                     FHIDRQDIKDFKNVYRGSKFDIKVKQKTGVPFIIKRVYKHKGMWICALVSLFLLMSTS
                     QFVTDVYIQSPEGIKKEALRNELYKVGVRPGVYKKSIDRKEVRDHMMSKFNDVAYLSI
                     NVKGTNIFVTVTKKAESLKSTDQSNYCNVIALKNGIIEKVIPRSGKSVIKSGDIVQKG
                     DVLLNGANTKSIPEVWASTFYESTKKASYVDTVNKKTGEKKNIYTLSFYDKEFTMRKN
                     IKYKDYVVENKEKKLSIGNYTFPIKIKTSTFYETKKVEVKRNKEELKKELSEKALKEL
                     EYIIPASARIIDVKHNFKVNKNMLEYLITVQTSENIAKIYPLSKSEAERFIKEESKPD
                     EGEEEVPSNPEKRPLNDIRNEFDEDNKDNKDQNNSDENNSNQNSNNSQNNNSNNN"
     gene            35581..36696
                     /locus_tag="PC471_000644"
     CDS             35581..36696
                     /locus_tag="PC471_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HDF2787785.1"
                     /translation="MKIIVIKIVIIVKIIIAITTNKIKLKILGGLIQLIIQKKFTIAD
                     GNFERELFGNFDENVKLIEKTLNIDVILREGNIILIGEEKNVDSALKLMNELHQTVSN
                     GKHLDKQSISYSLSLLLEGSEQKIKELEGTIVITQRGKAVQPKTLGQKEYIKLIENND
                     ITFGVGPAGTGKTYLAVAMAVKAFKRDEVSRIILTRPAVEAGESLGFLPGDLKDKVDP
                     YLRPLYDALFEMLGADKFNKYLERGTIEVAPLAFMRGRTLDNSFIILDEAQNTTSEQM
                     KMFLTRLGFGSKAVVTGDVTQTDLPQNKKSGLIQATEILKGVPGIGSIMLTDRDVVRH
                     ELVQRIIRAYEKHDKREEFKKEERKKAKMQEKTFKRK"
     gene            36753..37214
                     /gene="ybeY"
                     /locus_tag="PC471_000645"
     CDS             36753..37214
                     /gene="ybeY"
                     /locus_tag="PC471_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HDF2787786.1"
                     /translation="MDLILDDRQDKLEVSEELIEKIKDIIIECLDYEGYDDNYEVSLS
                     FVDNKEIHELNREYRGVDRVTDVLSFPLLSDDFEDVELEEESLGDIVVSLERALEQSI
                     EYNHSFEREVCFLICHSMFHLLGYDHDTDENTKEMREKEEHILNKLNITRE"
     gene            37220..37939
                     /locus_tag="PC471_000646"
     CDS             37220..37939
                     /locus_tag="PC471_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HDF2787787.1"
                     /translation="MKPEKTRQGIIKAFNAAIEGILYTFKFERNMKIHYLGSVAVLII
                     SLFFNFSKLEMIMLLMSICLVVVAEMFNTAIEKAVDLVTDEYHVLAKIAKDVAAGGVL
                     VAALNSVVVGYILFYDKLTDISGILIYKIRESELHITLICILLVLIAVVVVKALTSTG
                     TPLKGGMPSGHAALAFAIATAITLMTERVVASTLAYIMAVLVAQSRIEGKIHTFWETI
                     AGALLGVLIAILVFQLGMFYN"
     gene            38058..38459
                     /locus_tag="PC471_000647"
     CDS             38058..38459
                     /locus_tag="PC471_000647"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HDF2787788.1"
                     /translation="MDNIELLRLAEDARQHSYAPYSGFRVGAALLTKSGKVYTGCNIE
                     CASLGGTNCAERTAIFKAISEGDKDIYKIAIASDNSENNEQTYPCGICRQVIIEFGSD
                     IKIITGYTKGEVFEHSIKDLLPNYFSGNDLK"
     gene            38523..39416
                     /gene="era"
                     /locus_tag="PC471_000648"
     CDS             38523..39416
                     /gene="era"
                     /locus_tag="PC471_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HDF2787789.1"
                     /translation="MFKSGFVSIVGRPNVGKSTLMNNVVGEKIAIMSDKPQTTRNTIQ
                     AVYTDEEMQIVFLDTPGIHKPKNKLGEFMVKAATEAFKNVDLILFVVDDSKKIGPGDR
                     KIIEDLRSVKTPIILVVNKIDQLDQKDELFDIIKMYDREGIFKEIVPISALKGKNTDT
                     LIKVIQNYLEEGPKYFPDYMITDQPERVLIAELIREKVLHYLNDEIPHGVAVEIEKMK
                     ARNDKEIVDVSAVIYCERDSHKGIIIGKNGRKLKGIGKSARQDIELLLGSQINLQLWV
                     KVKENWRNLQNYINNFGYNDK"
     gene            39428..40807
                     /gene="mgtE"
                     /locus_tag="PC471_000649"
     CDS             39428..40807
                     /gene="mgtE"
                     /locus_tag="PC471_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HDF2787790.1"
                     /translation="MDRKLDASQDLLYEVKSLIDNNKVLELRELIEEYHIIDIFDIME
                     NLEEDMKIQLFEVLPLDMASSILEEGSVEFFISILSKLDVEHSKNILELMSLGDMADK
                     LSELEEEEREHIINLLNQENADYVKELLFYDEDSAGGTMTTGYISINKDMTALEAIDH
                     MREEAEEAETIYYIYVVDDEEKLVGVLSLRELIIARDANIVEDLMSENIISVYVDEDR
                     EEAVRLVSKYNLIAIPVVDRQEKLKGIITVDDIIDVMEEEATEDMYKFAGSSEHEREV
                     AEKENPTLREQIISALRGRLPWLIITLVGGLLASLILSNLDYIMNPVYAPLVFFIPVV
                     IGMGGNIGTQSSSVTVITLSNKDLNFSNVVREGIVGIITGLLCSIITGIVIYFVMRKL
                     DIVLIVSISLFINMVLGATIGAFMPVLLKKMDADPSTVSSPIISTALDITGIAVYFII
                     TTALLSKIV"
     gene            40880..41035
                     /locus_tag="PC471_000650"
     CDS             40880..41035
                     /locus_tag="PC471_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqzL family protein"
                     /protein_id="HDF2787791.1"
                     /translation="MNNISWEVFKKTGSIDAYLYFCDCKNIHEEIEEVREKKEDDDNP
                     EHPGDST"
     gene            41001..41774
                     /gene="recO"
                     /locus_tag="PC471_000651"
     CDS             41001..41774
                     /gene="recO"
                     /locus_tag="PC471_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HDF2787792.1"
                     /translation="MIILNTQGIVLKAIRYKESDIILTLFTRKLGKVSAIAKGAKKNK
                     SSLLSSSQLFSYSNFTLKKQGNMYKVTQSEIIKSFYNISYDIEAFSYATYITKLVENS
                     ILENQTNNRLFILLAQTLYLYTQDNTDNRFITAAFELKFLDYIGFKPIVNKCINCECK
                     ILQNSVFNIYEGGILCSKCSKLFDNNIKLDLTTISLMEYVLRNDILTCSKAKVSKYIT
                     HELENILDKYLKVYVDNINFKSLHVLQSVKNNKGVDNDE"
     gene            41767..42366
                     /locus_tag="PC471_000652"
     CDS             41767..42366
                     /locus_tag="PC471_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4342 domain-containing protein"
                     /protein_id="HDF2787793.1"
                     /translation="MSSNITIEMVDSVFERVPGATYAEAKEALVMCNGDVIEAVIYLE
                     NKKNTCNCKAKSAKETMEEVFGKDGENIKFQLKELLRKSSVVRIIVEKDGKTMMNIPL
                     TVGVVGLAFVPLATLVGLSAAVISKYRIKVQNEEDGEIVDLGELNEEKLHVLKNMITN
                     AAKDVKDVVVDKKEDDKDVTADLMKEESEKNKEDEDENK"
     gene            42526..43404
                     /gene="glyQ"
                     /locus_tag="PC471_000653"
     CDS             42526..43404
                     /gene="glyQ"
                     /locus_tag="PC471_000653"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HDF2787794.1"
                     /translation="MNFQEMILALQKYWSKQGCIMMQPYDIEKGAGTMNPNTFLRSLG
                     PEPWQVCYVEPSRRPADGRYGENPNRLYQHHQFQVILKPSPDNIQELYLESLKEIGID
                     PSEHDIRFVEDNWEAATVGAWGLGWEVWLDGMEITQFTYFQQVGNIECELETGEITYG
                     LERLAMYIQEVDSVYDLKWNDKITYGEVFNKAEYENSMYAFELCDADMLFNLFDIYEK
                     EALRLMENGLVIPSYDYVLKCSHAFNTLDARGAIGVSQRASFIGRVRNMAKTVAETFV
                     KQREEMGFPLLKDGDK"
     gene            43404..45470
                     /locus_tag="PC471_000654"
     CDS             43404..45470
                     /locus_tag="PC471_000654"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HDF2787795.1"
                     /translation="MKNYLLFEIGVEEMPSRFVGSTLEQLKNNLSKLFDENRIAFDKI
                     NAYGTPRRLTLVVEGLSDRQNDLEEEIKGPAKKIAVDAENNLTKPALGFIKSKGLSES
                     DIYFKQVGKEEYIFATIKQDGKETSEVLKDILPETIKSVVFPKAMRWGGKNLKFARPI
                     RWIVALLNDKVLEFDLEGIISSNVTKGHRFLGESTIEVDSIEDYFEKLEKNYIVLDQN
                     KRKEMIRKQCIEVANSLGGEVEFDEDLLEEVTYLVEYPTAFYGEFDVDYIKLPKEVVI
                     TPMKQHQRYFPVLKEGKLLPNFIAVRNGDSYRIDNVKAGNEKVLEARLADALFFYRED
                     TKRNLESYIEKLKTVVFQVKLGTIYDKTLRLEKLSADILDSLGLVDEKADTIRAAKLS
                     KADLVTGMVGEFDELQGFMGKEYAKVGGENDVVSEAIFEHYLPRFAGDILPKTNAGVA
                     LSIADKLDSIAGFFAIGIQPTGSQDPYALRRQALGILSILMDRKVAVDIKVLIEHALD
                     NYSELDFNKEEVVEQIMNFFNERVKNLFKDLGIRYDVVDAVLSSDINDVSDMHMRALE
                     LNNWLEKDELVEMLTAFNRVSTLAQKAESDKIDESLLKEDAEIKLYNEFKQVKVKVNE
                     LLKDKKYGIALDEFASLRPVVDNLFDTVMVMDNDEAIKNNRLALLKQVYDTMLGICDL
                     SKIVYK"
     gene            complement(45636..47183)
                     /locus_tag="PC471_000655"
     CDS             complement(45636..47183)
                     /locus_tag="PC471_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite/sulfite reductase"
                     /protein_id="HDF2787796.1"
                     /translation="MDNFKKILLNELPNFKEYSSKFLNGEINKMQYKGFSGGYGVYAQ
                     RDKKSFMIRLRVSAGVLSQYQLNKIYEIAIKHNLDKIHLTTRQAIQLHDLSINSIVDI
                     MEEGIKNDIFTRGGGGNYPRNVGLSPLSGVDPSEVFDVTPYAVATDKYFIKNATTYHL
                     PRKLKVSYSNCYTDTAHCSIQDLGFVATLKNDEPYFRVFLGGGLGKQPKVALELDELI
                     KPKDALYCVEGMIKFFMDYGNYENKNRARVRYMVESLGEKLFLEKFKEYYKLEKENGS
                     LELNIEEIDYSKPGVKIDIQDSRLIPQKQDGLYTVYIHPVGGILLTKDLSTLLKELDN
                     IENPMIRLGMTEGLYILNLNGNEAKRILEISKTISCNSQLEQSISCIGVPICQMGIQN
                     SQKMLHEIIDYFRLQNNKEILNKAPKLYISGCLNSCGVHQVGSIGLCGKKKNVDGIST
                     DAFELFVGGSFEIGKTRLGKSLGDFKASDIPEMLYKISDASSGNFYEWVNSNDNILNK
                     ITDKYKI"
     gene            47463..48230
                     /locus_tag="PC471_000656"
     CDS             47463..48230
                     /locus_tag="PC471_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HDF2787797.1"
                     /translation="MGEIINALDRALDIILLLYHEKREMGITEISKAMGVYKSTVHRT
                     LVTLENKGFVIQNAENGKYWLGINLYAIGMVVGEKMSLTEIVKPYTKKLNQEFNEVVN
                     VSILEERAQDSPRSIIIHKEYGSNQLLSVNPSVGSSSECYCSAVGKCLMAFNDSIDFE
                     KYRKTPIHKYTEHTIDNWDDMMLFLAKIKEQGYAIDDEELEHGLTCIGAPILDKNNKA
                     IAAISLSGPTIRMREGDFEYKIKRVIETAKSISELFR"
     gene            48513..48710
                     /locus_tag="PC471_000657"
     CDS             48513..48710
                     /locus_tag="PC471_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HDF2787798.1"
                     /translation="MEKVKLSVERCKGCYLCIEACKKDAISLSDYTNKKGFVTIKVDE
                     EKCIQCGACYTMCPDLVFEIL"
     gene            48739..49815
                     /gene="vorB"
                     /locus_tag="PC471_000658"
     CDS             48739..49815
                     /gene="vorB"
                     /locus_tag="PC471_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HDF2787799.1"
                     /translation="MQSLLIKGNEALAEGAIRGGCRFYAGYPITPQSEILEMMAWRQK
                     EVGGVFIQGESEIASVNMVMGASALGARAMTSSSGPGFSLKQEGIAYWVSAGLPAVVA
                     CVQRYGIGDGFIGAGQDNYWQAVKGGGNGDYHLIVLAPNSVQENMDMAYESFELAEKY
                     MHPVLILSDATIGQMVEPCIAPPMKEYDMDKAPWAAKGTPVGEKNKVITDITYFEDQD
                     TWQKGYIEKMRKVQEDEQRWEEIEIEDAEVVFVAYGISSRIAEGAVRDARAKGMKLGL
                     IRPKTLWPYPRKAFKKLSDSLKGLVTVEMNMMAQMKEDVIIACDNRFPVYSVATAQYM
                     AEVNQLIDFAQQIIDGKAKQEEVF"
     gene            49818..50537
                     /locus_tag="PC471_000659"
     CDS             49818..50537
                     /locus_tag="PC471_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate oxidoreductase"
                     /protein_id="HDF2787800.1"
                     /translation="MAKKAPAIMMCENMFCGGCGHSIVDRIIGEVLTEMDICQDTIIC
                     SDIGCNHMFQFSMNVDVVVPPHGKMGAAMAAMKRVRPDKYIFTIAGDGGAYAIGLGET
                     MSAATRNDNVIFLVMNNNMFAMTGGQMAPTTIEGQYTASTPAGRNYADHGSTFDVVKV
                     MGNLDIAYLARGTITNPKEINTTKRYIKKALEKQKEKKGFCLIEVLVPCPTNWGLKPV
                     DAMKRIENELVPKYPLGEFID"
     gene            50550..51089
                     /locus_tag="PC471_000660"
     CDS             50550..51089
                     /locus_tag="PC471_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HDF2787801.1"
                     /translation="MLNEIICAGFGGQGVLTAGKIIMYVAYKAEKKITWFPSYGNEMR
                     GGSANCNVVVSDEKIANPCVKNPDILLGMNSVSVDKYESLMKKNGYMFVNESLVSKDR
                     KYREDINVIKVPVTEIAQNLGNEKAANICMLGAMVKSLDLFTKEEFESGMCEYFENQG
                     KGKFNSKNVEAFEAGYDYE"
     gene            51246..52304
                     /gene="buk"
                     /locus_tag="PC471_000661"
     CDS             51246..52304
                     /gene="buk"
                     /locus_tag="PC471_000661"
                     /EC_number="2.7.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="butyrate kinase"
                     /protein_id="HDF2787802.1"
                     /translation="MKVLVINPGGTSTKISVFQDENEILKKNITHTREDLKNFSKVFD
                     EYDYRKQLIVDILSSENHSINSFDAVVGRGGLMKAIKGGTYTVSEEMIEDMRNEINGE
                     HASNLGALLAKTIADEIGVQSFVVDPVSVDEFDDVSRITGISDIEKASWLHALNHKAV
                     SRKIAQKLGGKYEDYNFIVAHLGSGISIVAHKQGKMIDGSGGRSDGPFSPERSGGLLA
                     YPLIELCYSGKYTHDEMVAKVSSIGGMYDYLGTKDMIEIENRIKEGDEKAKLIMDAFI
                     YQVAKEISMYGASLSGNVDRVILTGGISHSEMVVKGVAEKVSYLAPIEIVPGEMEMEA
                     LALGALRVLNGEEIAKTY"
     gene            52322..53215
                     /locus_tag="PC471_000662"
     CDS             52322..53215
                     /locus_tag="PC471_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional enoyl-CoA hydratase/phosphate
                     acetyltransferase"
                     /protein_id="HDF2787803.1"
                     /translation="MLKDFNNLLEEAKKLEPVIVSVAVAEDKEVLKAVKSATDLNIIK
                     PILVGDKDKIESISREIGLKGHEIIGCQNPDESVKKAVEIVKNKEAKVLMKGLVNTSV
                     YMRAILNKETGLRTGRLLSLLAVYELPQYHKLLYCTDSGINVSPNLNQKKDIMTNALL
                     TMKSIGMENPKTAILTANEMVDPKIQSTVDANLLVEMEKSGEIPKCIVEGPIAFDVAF
                     DAHAAEHKGIKSNIAGDVDLLVFPNIEAGNILGKSWLRFNKAKWAGIVLGATNPIILG
                     SRSDTAEIKINSIALACLASQ"
     gene            53245..54453
                     /locus_tag="PC471_000663"
     CDS             53245..54453
                     /locus_tag="PC471_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HDF2787804.1"
                     /translation="MKYAKRMDMVKASAIRDSQKKIAQKVASGGTVISFAAGLPDPNL
                     FPIEEISEITQKVLNERGKFALAYGPTKGEDELLDLLVKRMKEEENIDTKAENIIITT
                     GSQQGIALSAMILLEKGDVVVTENPSYLGAINAFRPYECDFLGVDTDEEGMVTDDLDK
                     LLSENSNIKVIYVIPTFQNPTGKTWSLQRRKDFMEVVNKYDVIVIEDNPYGEIRFTPE
                     PLPTLKSLDTKDKVLYLGSFSKVLCPGFRVAWMCGNVDIIDKAELLKQGIDLQSNQFS
                     QVQVIEFLKKYNLNEHIEKIRAEYKKRCLLMLDAMKKHFPEEAKYTEPDGGMFIWVEL
                     PESINVDDLLDKAIDAGVAYVSGESFFANNGPKNTMRLNYTTMSEEQIVKGVEILAEV
                     IKKELNYACE"
     gene            54477..55736
                     /locus_tag="PC471_000664"
     CDS             54477..55736
                     /locus_tag="PC471_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDF2787805.1"
                     /translation="MGEKENGIKFGKLMFQLVIIAISWELVYIIPFIQYTLYDPILKA
                     LECTNTQLGFLLTIYGLGNIFGAPIGGWLADRFDYRKIILGSVFLNGVVSFLFAFNMN
                     YPFAVATWVGCAITSLVMNYPSMVKILRVIGKDNQGKVYGFNEAMVGVSGVVMGAIFL
                     YIYTCFATPTLGMRWVMISLGILSIAMCPVLWFVIKDVDIKEEREEAGEKMSAGDFIT
                     VLKSPNTWLVGISIFCVYSFTVTMSYFTPYITSVLGGSVALSGALAIIRQHGLKLFGA
                     PFGGYCADKLKSPTKVLLPIYVFGIAVIVLFLVLPASTPMTIFIALTFVVGILGYMGK
                     GIYYAVQDEVKVPVKYSATTIGIAAALGFSPDVFQFALIGHWIDTYGNKGYTYTFIFQ
                     IAILVIGILSCLYILKVKKRRASKLEA"
BASE COUNT        22433 a         5938 c        10191 g        17667 t
ORIGIN      
        1 aaacaatata actaatatgt tagaaggtta tatgtaatta atgtcagtgt ttatatagaa
       61 tagcatttgt agcatttatt atagttgtaa ttactattta tatcatagac ttgaatagtc
      121 taaaaaaata gactataata tttttataga ctaaagaaag ggctgataag atggacatag
      181 caaaactaat tttaaatcct gcaaggcttc gtattttaca atatattcga ctttatggca
      241 gtgttcgtac atctgatatt gtaaagtatt taaatgatat tcctcgtgca acagtttatc
      301 accatgtaaa aattttagaa gagaacaata tgattgaagt aattaaagaa aatcgtgtgc
      361 gtggtactat tgaaaaggtt tatgcactaa aggaatatac aacatctatg gaaggagaga
      421 ccttcgttgc actttctaca gcattttatg taggtctgat gcaggaaatg aatgaatact
      481 ttagcagaaa agagcaagac cataaaaagg ataatgtatt tttctcttca gcattactat
      541 atatgagtga taatgaatat gaaaatcttc ttaaaagtat agctgattta ttgaaaccat
      601 atatagaaca aaaacctaaa tcaaatctaa aacttagaaa actttcaatt atatcttctc
      661 cacctgtagc tgataatgat aaaacaaaat aacatgcaac ccatttttat aaaaggaggt
      721 tatatatgaa atttcatgag tttggaaata aaaacagacc acatattatg ttgattcatg
      781 ggggagggaa tgcatggtgg aactatttgc gacaagcaga agttttgtct aacaaatatc
      841 atgtgatact tcctacattg gatggtcatg gagaagaata tgaaacaaca tacatttcta
      901 cagaggatac agcagataag ttgatatcat atatagatga aaattgcagt ggacatcttt
      961 tcgctttgtg tggggtatca cttggtggtc aaattgtcat ggaactttta tctcgaaaac
     1021 ctaatattac taaaaaagca attattgatg gtagtatttg ctacccaaga cctcttatgg
     1081 caaggttttg tattgctggt attcgatttt ttggaggact tttgtttagt gagaaggctt
     1141 gtcgctttca aattgctatg atgccaaaat tgttacctga aaatatgcaa tatccagata
     1201 aaataaaagc gtattatatg acagatatgc cacatgtaag aaaagaaaca ttctataata
     1261 tgtatcgtac ttacatgatg aactatacac ttaaagagaa tgttaaaaaa accacagcac
     1321 aagtaatgta ctggtatgga gaaaaagaaa tgaagtgtgt caaaaaatct gcaaaactgt
     1381 ttcagtctta tgtgccctct tgtgagattt atgaagctaa gagctataat catgggtatt
     1441 tatcacttta tcttccaaat gaatggttgg aaattgcaga accatttttc caaaaggaat
     1501 aaaggtgtta ggtgattatt gtataattat tgctgttttt ttatatagta aataaattag
     1561 gtataaatat aaaaaattat tttagttaat atattagtga aagcttaata ggaaaattat
     1621 aaatagatta aaggtagcac ctatataaat agttgctacc tttttctgtt tgtaaataaa
     1681 tgatagataa gaaggtttac tgacaaatca ctgactaaac ccaattttct tacattgaaa
     1741 gccttaattt tacttgtttt aaataaattt acaatttctt gttataaaat actaaatttt
     1801 gttaacaatt ataattagca tttaatttat actggaggtt tatatgatta gtgtaagaac
     1861 agatttagct ttagaagcta gtgaaatgtg tgaaaagtct caagaaggaa gtagtattcc
     1921 tggtgtaaaa atagaaacta aagaattaga aaattgcatt gtaactaaag ttgaagtaat
     1981 agacaggcaa ggcgcagaga taatgaataa agatatagga aagtatataa ctctggaaag
     2041 taatcttatg aagtttgatg atgatgagtc aagagaagaa atgataagtt atttaaaaga
     2101 ggagttagta gatatatttg gccaagacaa aaataaaaaa accctggtaa ttggtcttgg
     2161 aaatagaaat attacttctg atgcgttagg accaaagagt gtatcaaaaa cacttgtaac
     2221 tagacatctt tttaaaaatt ataataaaga ctatgatgat gatttcactg aggtatcagc
     2281 tttaagccca ggagttatgg gtgttactgg aatagaaact agtgaaatag taaaatcatt
     2341 ggttgagaag gttaagccag atagagtagt agcaatagat gcattggctt caagaaaaat
     2401 ggaaagagta aactctacta tacagatatc aacagcagga atatcacctg gtggaggtgt
     2461 tggcaatact agaaaatctc taactaaaga aactttaggc gtggatgtta ttgcaatagg
     2521 cgttccaacg gtggttgatg cagcaactct tacaatagat gtcttagata tggcaataga
     2581 caatttaata gctcaatcag aagaaacaga aagtttttat gaaatgttga aaaaacttaa
     2641 agaagaagaa aaatatcatc taataaaaga ttcgttagac ccatatgaca agaacttaat
     2701 agtaacacca aaggatatag atgatacaat cgaaaatcta tctataataa taagtgaagg
     2761 cttaaatagg tctttacatc caggtagatt ggtataaaat aaggaggagc aattatgttt
     2821 aaaaaatgca ttaaagtagt aaccttgact tttatattag tatgcatttt accaggaaaa
     2881 tcattagctt tgaatcaaga tgatttttta aagtttttag taaattcatc ttatccagaa
     2941 gctaaagtcg aaggaaatga tacagaaaat aaaaagaata ataaaaataa agaaacaagt
     3001 aaggaaaata aggaagaaag taaagaagga aacactaaat ctaaagatgc aagtaaagtt
     3061 gataataaaa aagagagtga aaaagaatat ataaaacttt atgttggtaa agaaaatgtt
     3121 ccagatattg agtcaaaaaa ttcagacaca actgaaacta acacaacatc tagttcagat
     3181 tataaggatg atttaagagt aaccaaagaa aatccaagaa ttttgatata tcatactcat
     3241 ggatgtgaga cttattcaaa ttcacctgat ggaaattatc attcaagaga taagaaaaat
     3301 tcagtaatgg aagtaggaag tgcattaact agtgctttag atagcaaagg atggggtgta
     3361 gtccatacta ctaaatacca tgactatcca tcttataata actcttatgc aagtagctta
     3421 aaaacaatac aaagtatatt acctaaatat aactcagtgg atatagcgat agacttacat
     3481 agagatgcta gggatttaac taatccagcc actaaggaaa aagaccattt aaaatataca
     3541 actatgataa atggagagag agtatcaaag ttcttctttg ttgtaggagg taaaaataca
     3601 aatagaaaac aacttagagc tttagcagaa gatataactg cttttgctga gaaaaagtat
     3661 cctggtctag tatctccaat agtagaaaaa gactatgcta gattcaatca gtttgcagtt
     3721 aagaatcata tgttagtaga aatagggaat aacgcaacaa gtgtagaaga atctaaggct
     3781 actacaaagt atttagccga gattttggat gaatatttta aacaaaaaaa ttagagcttt
     3841 aagttataat taggagcatg ctaaatgagc cgattagaaa aaaatataga aagtaaaagt
     3901 aaactaagaa aatacaagta tattatgaga atcttatttt tgattactat gattatatgt
     3961 acatcagttt gtgtatttat agtggacagt aatgcaaaaa gtatgctagg aatgaaacaa
     4021 tatgattttc caagtattaa atttgatata acaaaatttg attttgacat accaaatttt
     4081 gatatacatg aaatcataaa aaaatttaat aagtagtaat aagtgaataa ggtttattga
     4141 ttaaaataag ccttatttta gttatatatt tttaatgtat aaaataaaaa ttcattaact
     4201 gtattttata ggatttactt taagtttatg atattatata atatttagtg ttagacttga
     4261 taatccacat ataatgtgga taattttgtt tagtgccaat tagtttatat tcaaaaattt
     4321 taaatttggt atataaagag tttttagata atgtaaaata ataactataa gaataatcaa
     4381 gaggagggaa ttttaaggtg gacaataaac aaagtagaac tagaaatttt agtataattg
     4441 cacatataga ccatggaaag tctaccttag ctgatagatt aatacaacaa actgggcttg
     4501 ttagtgaaag agacatgaaa agtcaattac ttgacaatat ggaccttgaa agagaaagag
     4561 gaatcactat aaagcttcaa aacataagat taatgtataa agcgaaggat ggaaatgaat
     4621 attatttaaa tcttatagat acacctggac atgtagactt taactatgag gtatcaagaa
     4681 gtttagctgc atgtgaagga gcactattag tagttgatgc agctcaaggt gttgaggcac
     4741 aaactttagc caatgtttat cttgctatag accaagattt agaaatatta ccaataataa
     4801 ataaaataga tttaccaagt gcaagaccag aggaagtaaa aaatgaaata gaagatttaa
     4861 tagggcttga ttcaagtgaa gctcctctta tttcagctaa aactggttta aacatagaag
     4921 atgttttaga agatattgta aaaaatgttc ctccacctaa aggagataat gaagctccat
     4981 taaaggcatt aatattcgat tcttactatg atgcttacaa aggtgttgta gcttatgtta
     5041 gagtatttga aggaactgtt aaaaagggta tgacaataaa aatgatgaat accaataaaa
     5101 agtttgaggt aactgaagtt ggtgttatgg ctccaggtca aactgaactt agtgaattat
     5161 cagcaggtga tgttggttat attgctgcta gtataaaaga tataagaagt tgtcgtgttg
     5221 gtgatacaat aacagattca aataatccta cagaagaacc tttaccagga tacaaaaaag
     5281 ctactccaat ggtatattgt ggtatatatc caggagaagg agaaaagtat gagaatgtta
     5341 gagatgctct tgaaaagctt caagtaaatg atgctgcact tgaatatgaa gctgagactt
     5401 cagcagcact aggatttggt tttagatgtg gtttcttagg tcttttacat atggagataa
     5461 tgcaagaaag acttgagaga gaatttaacc ttgatataat tactacagct ccatctgtta
     5521 tatatagagt tacaaaaatg gatggagagg ttgtaatgat acaaaatcct gcaaacttac
     5581 cagaaccatc tgagataaag atgatagaag agcctatagt aaaaggagat ataatagttc
     5641 ctaaagacta tgtgggtgtt gttatggagc tttgccagga aagacgtgga aatatgttaa
     5701 atatggaata tatagatgat agaagagtaa tgcttcacta tgaccttcct ctcaatgaag
     5761 ttgtttacga tttctttgat gctttaaaat caagaactag aggttatggt tctcttgatt
     5821 atgaggttaa aggttatgta gcatctacac ttgtaaaact tgatatatta atcaataaag
     5881 agcaagtgga tgcacttagt tttattgtcc atgagacacg tgctttccct agaggaaagg
     5941 caatgtgtga gaaattaaaa ggagaaattc caagacatca gtttgctata ccaatacaag
     6001 ctgctgttgg taataaggtt attgcaagag aaacaataag cgctctaaga aaagatgttc
     6061 ttgctaaatg ttatggtgga gatatatctc gtaagaagaa acttcttgaa aaacaaaaag
     6121 aaggtaagaa gcgtatgaga caaattggtt ctgttgaagt tcctcaaaaa gcatttatgt
     6181 ctgtgcttaa attagatgag taaatcctta taaatttaga taaagatatt taaaagctag
     6241 agtgatttta gaattatttc taaattagtc tagctttttt atggtatatt ttaagtaaac
     6301 tcatagagaa tataagtaag aatattaaag aagaataaaa aggtgatatt tatgattaat
     6361 atagaaattc ctaaaaaagt agactatata attaaagaat tagaaaagaa tggttatgaa
     6421 gcttatatag ttggaggatg tgttagagat tgtttattag aaagagttcc aaacgattgg
     6481 gatataacaa ctagtgctag accagaagtg gtagtagaat tatttgaaaa aacaattcca
     6541 actggtatac aacatgggac tgtaacagtt atgattgaac atgaaccatt tgaagtgacg
     6601 acttatagaa ttgatggaaa ttatagtgat ggtagacatc cagattcaat agaatttaca
     6661 aataacatag ttaaggactt gtcaagaaga gattttacta tcaattcaat tgcatacaat
     6721 tcaaagacag gattagtcga cccatttaat ggctatgaag atatacaaaa taagtatata
     6781 cgttgtgttg gaaatccagt agatagattt gaagaagatg cacttagaat gcttagagca
     6841 gttagatttt cagcacaact caattttaaa atagctgaag gtactaagca aagtattcat
     6901 aaaaaagcag atttgataaa aaatgtttct attgaaagaa tccagacaga gtttaataaa
     6961 atattagtat ctgattcatc taaattaaac ttattaaaat caacaggttt gttgaaattt
     7021 ataattcctg aaatatgtga attagaagat gttactcaac acaatccata tcacatctat
     7081 gatgtacaaa aacatacttt aatagctaca gaagtaattg aagatgaact gtatttaaaa
     7141 cttacaatgt tgtttcatga tttaggtaaa aaagtaacta aaacaactga taaaaatggt
     7201 gttgaccatt tttatactca tagtagagaa tctgtaaaga ttgcaaagaa gattttgaaa
     7261 agacttaagt atgataatta tactattaat aaagttttaa tattaataca atatcatgat
     7321 tatagaatag aacctaaaag gaagataatt aaaaagttat taaataaact tgaagatgtt
     7381 gaattatttg aagatttaat aaaagttaat tgggctgata cacttgcaaa aaatccaaag
     7441 tatgcaaaac aaaagatatt aaatcttatt gagtgtgaaa aagaatttaa acatataata
     7501 aatcaaaagg agtgctttaa tcttaaagat ttagctatta atggaaaaga tttgatatct
     7561 ataggtataa aaccaggtaa agatatagga tatattttaa ataagatgct tgaaatagtc
     7621 ataaataatc cagagttaaa tgaaaaagaa atactaaaag aaaaagcatt aaatatatat
     7681 acattttaga ttcaaatata aaaaatttaa ttactttaaa aaacatagaa taaaatactt
     7741 atttagataa aataaataat ttttagagaa tattttagaa gttacgattg tatgattttt
     7801 aaatgcatag gcatttactc taggactata gactaatata aaaaaaaact ttgggaatac
     7861 taaaaccata aatttataaa attaatattt tatatactgt aaaacagtga ggagggacag
     7921 tatgcatact caactacaaa agactattgg tctttcagca gctctttcta ctgttgtagg
     7981 tatggttata ggttcgggag ttttttttaa gccacaagca atatacacta caactaatgg
     8041 agctccaggt ctaggtatta tagcgtggct tttaggtggt tttataacaa taactgctgg
     8101 gcttacagct acagaaattt cagctgctat accaaaaact ggtggtatga tgatttatat
     8161 cgaagaaata tatggagaaa agttaggttt tttaactgga tggatgcaaa ctgtattatt
     8221 tttcccaggt acatcagcgg cattaggagt aatatttgct caacaagcat ctgaactttt
     8281 aggaatgagc ccaaataata tggcaaatgt acttcctata gcaattggag ttatactatt
     8341 tttagcgtta ttaaatataa taggttcatc cttaggagga aaagttcaga cagtagccac
     8401 tattggaaag atgatacctt taattttaat aatagtcttt ggatttatta aaggtcaaag
     8461 tagtgaagta ttaaatccat ttgtaggaga tggtgtaaat gcatcaaatg cacttggaca
     8521 agtattaata gctactttgt ttgcatatga tggttggata aatgttggag ctatatcagg
     8581 agaaatgaag tctccagaga aagatttacc aagagcaata gttggtggtt tatcattagt
     8641 tatggcagtt tatattataa taaatatagc ttatctttgg gtagtaccag caagtgaatt
     8701 agcaacagtt acttccccag ctacattagt agcaacaaga ttgtttggaa atataggtgg
     8761 aaaagtaata actgtgggta tattaatatc agtatttggg acattgaatg gatatttatt
     8821 aacaggttca aggatacctt atacattagc tgaaatggga actttaccag catctaaaac
     8881 tcttttaaaa gtaaattctg gaggttctcc tgtaaattca atactactta taactgtgtt
     8941 agcctgtgta tatgcattat ctggtcaatt taatcttttg acagacttaa caatattttc
     9001 aatatgggta ttttatgtat tgacttttat aggtgttatg aggcttagaa gacaaaagcc
     9061 agatttacac agaccatata aagtcccttt atatccaata ataccaatta tagctatatt
     9121 aggtggactg tttgtaataa taaatcaaat attgacttca acagtcatat ctttaggcgg
     9181 aatatttata acactattag gtttaccagt ttattattat atgaaaaaaa gataactact
     9241 taatttaagt tagataaatt tgggtaaagg tgaaaattaa cttttatcca aattttttat
     9301 actgttatac aatataagta ttatatattg cttgatgtgc tattttgttt gatttataat
     9361 ttagtaattg atgttttaag ataatttaag aaatgaggag tgaaagatgc taggacttta
     9421 tgtacatatt ccattttgtg tgaaaaaatg taagtattgt gattttaatt cttataaaat
     9481 ggacatagat tcaaaaaaaa gatatataga agacttaaaa atagaaatgg aattatatag
     9541 taataaatta tataaagata ataaatataa aaataaagaa tgctgtagtc taaataaaaa
     9601 tgacaaaata acgagtatat ttgtaggggg aggtacacct agtatactaa cctctgatga
     9661 aataagagaa gtatttataa gtataaaaga aatgtttgat atagatgaga atgcagaaat
     9721 aactatagag tgtaatcctg ggacattaac attagaaaaa ttaaaaacta tgaaagagat
     9781 tggcataaat agacttagta tagggctaca agctattcaa gaaaagcatt tgaactttat
     9841 aggaagaata catacttatg aagaatttga gaaaaactat aaagatgcat taagtgtagg
     9901 ttttaaaaat attaatatag atttaatgta cagtcttcca aatcaaactc tatgtgattg
     9961 gaaggaaact cttgaaaaag ttgtacactt aaatccaaca catatatcag cttattccct
    10021 tatactagaa gaaggaactg aactatacaa tatgtatgaa agtaataaat ttgaacttat
    10081 agatgaaaat gtagatatag aaatgtatga gtatacaata aactatttaa agtcaaaagg
    10141 atataatcaa tatgaaatat caaactactc aaaagaaggc tataattgtg agcataatat
    10201 tttatattgg gaatgtgaac attatatagg gattggagca ggagcttctg gctatattaa
    10261 tgagaacaga tacaataatg tagaatcttt agaagattat catttaagtt tggtaaaaag
    10321 agaaaaacca atacaagaaa atgaaattct ttctgagaaa gatatgatag aagaaaagat
    10381 atttatgggg cttagaatga ataaaggcat aaaatttgaa gattttaaaa aaaaatttgg
    10441 aatagatttt agagagaagt ataataagca aatagaaatg ctattagcta gaaagcttat
    10501 aaatcagagc tttgaaggta tacaattaac tcaaaaagga agagaaatat ctaatagtgt
    10561 atttatagaa tttatggaat aatttttaag gtgttgacaa aataaattta taatggtata
    10621 taatatatat aatcttttta gcactcgaac ttagtgagtg ctaacagcta ggaggtataa
    10681 taatatggaa ttaaatgaaa gaaaattaaa tatcctcaaa gctatagtta aagattatat
    10741 agaaacagct gaagctatag gttctagaac aatatctaaa agacatgatt taggtgttag
    10801 tgctgctacc ataagaaatg aaatggctga ccttgaagaa ttagggtatt taattcagcc
    10861 tcatacttct gcgggtagag ttccatctga aaaaggctat aagttatatg taaactctct
    10921 tatgagtaaa agcgaattgg atgataatga taagattctt attgaacaat gtatgaatca
    10981 taatataaat catataaaag aattgattca tgaaacttct aagctattat cgcaactaac
    11041 aaactataca actgtagcag ttactaagag tttaataaat caaagtgtta taaagcacat
    11101 acagttggtt gctatgaatg acaataatat tttacttata gttgtaacag ataaagggga
    11161 cttaaaaaaa gctaacctta caactaatgt atatttagat caatctaagt taaatttgat
    11221 ttctgataat cttacaagga agctcttagg taaaagtata acagatttgg atgataatct
    11281 aattgctttt attaaatacg aaataagtga gtattctggg cttatagatg agcttttaaa
    11341 tgctttgaac tcaaatatga aagaagaaga tttttctcta tcattaaatg gagctactaa
    11401 catatttagt tatccagaat ttaatgatgt attaaaagca aagtcatttt taaatatgtt
    11461 ggaaaaaaag gaaacaatag ctgacataat taaatcaaag ggaatacaaa aagacaatct
    11521 aaatataata attggtagtg ataatgattg tgaactggca caggattgta gtatagttac
    11581 agcaacttat aatgtagata gagacttggt tgggcgaata agctttatag gccctaccag
    11641 aatggattat gctagaattt actctataat aaattatatg agtttattaa ttaatagaaa
    11701 gtaaagaaat tttaaattgc gaggtgtaaa gtcttggata aaaaaaatga gcaacaggaa
    11761 gtaagagaag aaaatgatac ttctattaac caagaatcgg aaactcaagt agagttagaa
    11821 gaggaagttg ttaatgaaga atgtgaaaca tcaagtgaaa aaactgatga aaaagaagtt
    11881 gatgatgaaa atgtaacaga tataaattct aagttagcag aaaaaaaatt gcaggatgag
    11941 ttggatgaat taaatgataa atatcaaaga cttcaagccg aatatgcaaa ctacagaaga
    12001 agaactcaac aagagaaaga gacaataggc gtatttgcaa atgaaaaaat aataactgaa
    12061 ttgataccag ttatagatag tatggaaaga gccttagatg catgtgaaga taaggaagat
    12121 actatgtata agggtataag ccttgtacat aaacaattaa tagacacttt agtaaaattt
    12181 ggagtagaag aaatagaagc agaatctaag gaatttgacc ctaatctaca tctagcagtt
    12241 atgcaagaaa gtgtagatgg agttgaagca aatcaaatag taatggtact tcaaaaagga
    12301 tataagctag gaactaaagt tgtaagacct tcaatggtta aagtttcttg ctaagtataa
    12361 attaaaggtt aaattattta acctaatata aaatattaaa ataagtaaaa cttaattata
    12421 taattactcg aggaggattt tagtcatggg aaaaataata ggaatagatt taggaacaac
    12481 aaattcatgt gttgcagtgt tagaaggtgg agaagcacaa ataatagcaa atagtgaagg
    12541 tatgagaact actccatcag tagtagcatt tacaaaagat ggtgaaagaa tagttggaga
    12601 acctgcaaaa agacaagcag ttacaaatgc agataaaaca ataacttcta taaaaactca
    12661 tatgggaact gattataaag taaatataga tggaaaatca tatactccac aagaaatatc
    12721 agcaataata ttacaaaaat taaaatcaga tgcagaaagt tacttaggac aaacagtaac
    12781 agaagcagtt ataacagtac cagcttactt tacagatgct caaagacaag caactaaaga
    12841 tgctggtaga atagcagggc ttgatgttaa gagaataata aatgagccaa cagcagcagc
    12901 tcttgcttat ggtatggata aattagacca agaaaagaaa atattagtat ttgacttagg
    12961 tggaggaact tttgacgtat ctatacttga aataggagat ggaacttttg aagttttagc
    13021 tacagctggt aacaatagat taggtggaga tgactttgac caaatagtaa tagattattt
    13081 agcagaggaa tttaaaaaag ctgaaggtgt agatttaaga aatgataaaa tggctcttca
    13141 aagattaaaa gaagcagcag aaaaggctaa gaaagaatta tcatcaacaa tgagttcaaa
    13201 cataaactta cctttcataa ctgctacagc agaaggacca aaacacttaa atatagattt
    13261 atcaagagct aaatttgaag aattaactag aggtttagtt gaaaaaacta tggaaccaac
    13321 taagacagct ttacaagatg caggactttc tacaggtgat atagatgatg tattattagt
    13381 aggtggttct acaagaatac cagcagtaca agaagctgtt aagaagttta taggaaaaga
    13441 acctcataaa ggtataaacc cagatgagtg tgttgcagca ggtgcttcta tacaagctgg
    13501 agttttagca ggggatgtta aagacttatt attactagat gttactccat tatctcttgg
    13561 tatagaaact atgggtaatg taatgactaa gataatagaa agaaatacta caataccaac
    13621 taagaagtct caaatattct caactgctgc tgataaccaa acagctgttg atatacatgt
    13681 tcttcaaggt gaaagaagta tggcttatga caatacaact ttaggaagat tccaattaac
    13741 agatatacca ccagcacaaa gaggaatacc tcaaatagaa gttacttttg atatagatgc
    13801 aaatggtata gttaatgttt cagctaaaga tttaggtaca ggaaaagagc aaaaaataac
    13861 tataacatca aacactaatt tatcagaagc agaaatagag caaaagataa aagaagcaga
    13921 aatgaatgca gaagctgaca aacaaaagaa agaaaaaata gaagcattca atcaagctga
    13981 atctactata tatcaaactg aaaaaacttt aaatgaactt ggagataaaa taagttcagg
    14041 tgaaaaagag gatatagaaa aagctatagc agacttaaaa gctgtaaaag ataatcaaga
    14101 tgctacagca gaagaactta agaaagctac agatgaagta atgactaagt tccaaaaagt
    14161 atctcaagaa atgtaccaaa aagcagctca agaacaacaa gcggcacaag gtgcagaaca
    14221 agcacaagac aatggaccta aagatgacaa tgtagtagat gctgacttta aagaagtaga
    14281 tgaagataaa taagctatat aatatgtaac actaaatgat aattggaaca tatatgattt
    14341 gtgaaaatca tatagatgtt aaagatatat ggacaatata catcataaag aatataatat
    14401 atcatagatt atataactta aagtagaata taagaattaa tgatagcttg tagtttatta
    14461 ttctacaagc tatcgttttg taaaatgaag gtggtgtcaa tttttgagta caaaaaggga
    14521 ttattatgaa gtcttaggta tcagtaaagg tgcagaagct caagagataa aaaaagctta
    14581 tagaaaatta gcaatgaagt accatccaga tagaaatcct ggagataaag aggcggaaga
    14641 aaaatttaaa gagataaatg aagcttatga agttctatct gatgatacaa agagaaaaac
    14701 ttatgaccag tttggacatg atgggctaaa tggtcaagga ggatttggcg gtcaaggctt
    14761 tggtggtttt gaagatatgt tcggagatat atttggtgac atgtttggag gaagcttcgg
    14821 aggtggaaga gcaagaagaa gagggcctca acgtggtgca gatataagac agagtgttac
    14881 tatatctttt gaagaagctg catttggtaa aaagatgtct ataaaagtaa atagaagtga
    14941 agaatgtgaa gaatgtaatg gaactggagc aaaaccagga acatcaaaga aaacttgttc
    15001 aacttgtaat ggaactggtc aagttagaac tgttcaaaga actccatttg gtaatatagc
    15061 aagttcaaga ccatgtagtg cttgtaatgg aactggagaa gtgattgaat ctccatgtag
    15121 taaatgtcat ggaactggaa atacaagaaa agttaagact atagaagtag atatacctgc
    15181 tggtatagat gatggtcaaa tgataaaact ttctggtcaa ggtgaagtag gagaaaaagg
    15241 tgctccaaga ggagacttat atattgtagt taatgttaag tcacatccac tatttactag
    15301 agatggaaat gatatttatt ttgaaatgcc aattacattt gttcaagcaa ctttaggtga
    15361 tgagatagaa gtacctactt tggatggaaa agttaaatat agtgtgccag aggggacaca
    15421 aactggaact gtatttagac ttaaagagaa aggaatccct agaattagag gtaattcaag
    15481 aggagaccaa tatgttaagg ttgtagttga aattcctaaa aaattaaacg ataagcaaaa
    15541 agaactattg agagaatttg cgaaggagtg tgggtcgaac gtccacgaaa agaagaaaac
    15601 cttcggacaa aagatagaag atatgtttaa gaaaaagtag ttaaagtagc caatatataa
    15661 tcatattttt aaaatcttag ttatttataa gattgtcaaa ggagatatta tgatttttat
    15721 attggctatt tttgtataaa aaataaatgt gcataaaata aaactgaata taaataattt
    15781 tctgaaaaat acttgtccta aaatatataa tatgctataa tataatcaaa taaaagaaag
    15841 gggtgattcc agtgggaacg gatgtggcac tgccaaatga tgaagaaaac tttattgtaa
    15901 tatacaaaat aaaattagat acttttagtg ctagattaag tactggaaaa actcatgagc
    15961 tgggataatt attgtaatta cctagactca aacttttagt agtaaaatag taatattata
    16021 tagacaaggt atctaatttt taaatctttt gtatgaatta aaatttttat aaagggtata
    16081 tctgattaga atgtaattag ggaaacctag ttgtgaatta cttttagtat atacatgtgt
    16141 aaagaaatat gtatatggta ttggttattg tgctagatgt gtatgtaaag gaatatgtgt
    16201 ctagttgggt atatgtaaag aaatatactt ataatctatg aaaaattttt agtataaatt
    16261 gctaaaacat tgagattgtt ttttatagaa agttagttta taccaactaa aaatgtgctt
    16321 aagatttgtt tgatgtttaa ttataaataa ggttgtagat aaaaaaataa gtaaagacaa
    16381 aggtttaaaa tacttgcttt aaatatagtt tgttttgaac acttgccaaa ttaaaataat
    16441 taataaaata gacatcttct catagtaaaa tatactgtga gaggatgttt ttgctttgtt
    16501 cataaacttt tatgaaaaat ttgcatagtg tcacatttta atatactata tatataataa
    16561 aatatagata aaattaaatg attcaaaaga gaaaggtgaa aagagatgta ttatataggt
    16621 gtagacatag gaggaacagg tatacaagca ggagtagttg ataactatgg gaaaataatt
    16681 tttaggtcag agtgtaaaac agtaattgaa aaaggctttg aaggaatact taatgacata
    16741 aaaataatga tttataagtt gttggaagat aataaactta ctatgagtga cataaaatct
    16801 attggatttg gtgttccagg ttttataaat aaagaaggat tagtaacttg tgtaaattta
    16861 aaatggaata aaaaagcttt taataaagag ttaaaaagaa gatttccaga tgttgaaatt
    16921 catggagaaa atgatgcaac agtagcagca cttggagaag caaagtttgg aagtatgaaa
    16981 ggtgctaatg ttggagtatt atatacttta ggaactggag taggtggagg aatagtaatt
    17041 aatcaaaaag tcttttctgg agcacatgga ttgggttcag aaataggtca tcaaattata
    17101 ggagaaaact attttaattg taattgtgga aataatggtt gtgtagaaac tttttgttca
    17161 gcaactgcaa ttataaagta ttctcaaaaa ctgatagaag aaggagaaaa gagtaggatt
    17221 ttagatttgg cagagggaaa tttagaaaat gtaaatgcaa agatggtgtt tgatgcatat
    17281 agagaaaatg atttagtggc aattaaagta ataaatagat ttaaagagta tttagcaaaa
    17341 acttttgcaa acacaataaa ttctttagac ccagaaatta tatctatagg aggaggcatt
    17401 tctaaatcta gcgatataat acttgatgga atagaagatt tggtaagaaa atttgtatta
    17461 tacaaaacag aagatatagc tacaattaca tgtgcaactt taggttcaga cgcaggaatt
    17521 ataggagcag cttttttata atgtaaatga actgcttatt ggaggcgtga tgatatatgg
    17581 gtaaaacaaa tgctagaaga acgctttttt tgatagcaat aggttcagga actatgctga
    17641 atcctcttaa ttcttctatg atttcacttg ctttacatag tatacaaaat gattttcata
    17701 ttagttttac aactgtatct tggattgttt cagcatttta cctagctagt gctattgcac
    17761 aaccagtttc aggtaagata ggagatttaa taggtagaaa ggtcttattt ttgagtggtt
    17821 tgatgcttgt aattttatca gcatccatag taccacttgt tcaatcattc tttatattga
    17881 tttttataag agtaattcaa gcaattggaa caagtaccct atatccatct ggagttgctc
    17941 ttatacaaaa taatataaaa gaaagacaat cttctgcatt agcagttcta accatttttg
    18001 cttcaactac agcaggatta ggtccgacac ttggaggatt attgttggac ttaggaggat
    18061 ggcatgctat atttcttgta aatatacctg ttgttttggt ttctatatgc ttagggtatt
    18121 ttttattccc aaaagaagta aaagaaaaga aatctataaa agaaacttta aaaaatgttt
    18181 tgtcaagatt agatattatt ggcatacttc tattttcaat tgcaatgatt tttatattac
    18241 tatttttgct atctatgaag gagtctttta acttggaaca acttgtattt ggtataatac
    18301 tgatgtgtct ttttatttgg catgaattga gaacaaaatt gccatttata gatattaagc
    18361 tttttgtaag taatcctaaa ttgtcaaaag tttacttgca atttattata ttaaatttct
    18421 ttaattatat tttgtttttt ggtcttccta gttattttca agatgcctta cattatagtg
    18481 caaagtcgac tggattattt atgctattta tgtctggtat aggtatattt atatctccat
    18541 taactggaaa atggatagat aaatctggaa ctagatttcc tcttgttaca tcatcaatct
    18601 ttatgtttat tagtgcagta cttctttcag tagtttttat ccatatacct gtaataggaa
    18661 agggtattat attatcatta gcaggtatta gttatggagt tggtaatgta gccctgcaat
    18721 cctctatgtt tgaagaaagt cctcgtgaca gtatagggac agcttctggt ctgtttcaaa
    18781 ctagtcgtta tttaggttct attttagcaa ctgttttact tggaatggta tttgaagtaa
    18841 atataacatc agaacaattt cagatattag gttatgttat ggtggtctta ggtacaataa
    18901 gttttttatt aaattttata ttaaagaaag aattaagaaa agtagaatag tggatttatg
    18961 gatttaataa gtctaataac tgataagttt aaaaatattg tttgtaaaac ataactggtt
    19021 tgatatttat taattctaag ttttgaagca tagagtactc gataagtata caatattttg
    19081 aattaaaatt aagcagaaat ttgaactagt ctttcaaatc tctgcttaat ttagtagtta
    19141 aattttttta tagagaaaat aagatttgac acttagataa aaaatcattg cattttcaaa
    19201 attctttaaa tcaaacccaa gtatctcatt aatcttttcc aatctatata ctagcgtgtt
    19261 tctatgtaaa aacatactag tagatgtctt agaaatattt aaatcattat ttataaattt
    19321 tattcctgta tatagaagtt cttcgtcaat agatttaatc ttattaaaat ctattaattt
    19381 attgagtata ttatcctcat taattaaatc taaagtttta tatctaataa agtcaaacaa
    19441 atttaaaaac ttattttcag cgttgtcttt tataatatga ggtagtaaat cagaacataa
    19501 ctgtaatcta aaatcaaaat cagctttact atctaattca ccaagataaa aaatataagt
    19561 actttgataa gtttcagttt caattatatt ttgtgtagtt acattaatat ctaagttata
    19621 tttagaattt actataaaaa aaccattgtg ttgtattata tttatatctt caaataaatt
    19681 ataaaaaata ttttttagat attcaaagtc tatatgagat tggtaaatta aataaaagtt
    19741 gccttttaaa ttatcgagat tatttttatc tagaaaatca ataacataaa tagcattatt
    19801 gaaagtaact aaatggctac tagcatctaa cttttggttt tcatatggat agatatttat
    19861 ttttttatta agctgtcttt ctaaaaaatc ttttaactct tccataaagt ctccttaaaa
    19921 ataagattat attcttatct tatagctaaa gtagtttctt tgtcaaatat gtgtactcta
    19981 ctattgtcaa ctgaaagtgt taagtcatca ccaacattaa ttctgttttt actgtcaaat
    20041 ctaattgtta tcgtattttc gcctagtttt aagtgagcat aagtttctgc tcccataagt
    20101 tcttttattt caattcttga tttaaaacaa gtatctggat tagatttaat aaaagtatct
    20161 tctacatgaa tatcttctgg tctgatacct ataacaactt cattattaat ataattgtct
    20221 tttattaaat catagtgttt ttctttattc aatttaagct taatatgttc attttgtgca
    20281 taaatttcat cattttcttc tattaatatt acatcaataa aattcatctg tggtgctcca
    20341 ataaatcctg caacaaatat attagctgga taatcgtata catcttgtgg agttgcaatc
    20401 tgctgaacta ctccatcctt cattactacg attctatcag ccatagtcat agcttcaact
    20461 tggtcgtgtg ttacatagat aaatgtagtt ccaagctgtt ggtgtagctt agttatctct
    20521 gttctcatag cagttctaag ttttgcatct aagttggata gaggttcatc cattaagaat
    20581 accttaggat ttctaactat tgcacgtcct aaggcaaccc tttgtctttg tcctccagat
    20641 aatgttttag gttttttatt taaaagaggt agtaagtcta atattttagc agcttcttta
    20701 acttttttgt ctatttcatc tttaggcaaa tttctaagtt taagtgcaaa tgccatgttt
    20761 tcgtaaacag ataagtgggg atataaagca tagttttgaa aaaccatagc tatatctcta
    20821 tcttttggtt ctatattatt gacaagttta tctccaatgt atagttctcc ttcagagatt
    20881 tcttcaagac cagcaatcat tctaagtgta gtcgatttac cacaaccaga tggtcctact
    20941 aaaacgataa attctttatc tttaatgtct atatttatat tttttactgc gttaaatcca
    21001 tttgagtaaa gttttgatat attttttaaa attacttctg acataatatc ctccttgata
    21061 tttaaatttt aattatatag tatcaaattt ttttattata ttaaatgtgt aaaatgacta
    21121 aatgtatttg tttaatatgt atatttagtt tttagcaaag aataaaacta acagtagaat
    21181 atatttaaaa aattaaaaaa caaaattaaa tttattatca aaaaatcatt ttatgatatg
    21241 ttttattttt atattacaaa tagagaataa tgaattatac tagaaagtag gaggtgaaaa
    21301 tatgagcaga atttcaattt ttatggaatc atgtaatggc aaatttccaa atgaaaattc
    21361 aatgttgtca gtttccttta taaaaaatat tttaaatgca gaaaacaaag attttgctaa
    21421 aaacatattt aactatgggg aagaaagaaa aaacaataag caaataaagg atataaatac
    21481 agccatttac ataccaaatc ttataagaag caaaaatgag ttacttgttg atggagatat
    21541 aatattcaat ataagttttt ataattattc tatgttcagt aaattataca atggaatttt
    21601 aaaagtaaag gagctagaat ataaaggtta tagatttaaa ataaaaaata taaaaataca
    21661 taaagaacac gagataaaag aaaatggtgt tatatttaaa actatgtctc ctataatagt
    21721 aaagaataaa gaaggtaaat atttagatgt agaagattca aattatatag aatgtttaaa
    21781 ctatatagct aatctaactc tggagagtat aagaggtagt gggcttagaa agccattgga
    21841 atttatacct attaacttca aaaaaagagt gttaaaagaa aagattcgtg gatttaaagt
    21901 gagagaatat tactatataa atgcttatgc aggaacattt tttttaaaag gagatatgga
    21961 agatttaaat gcactgtata aaatgggaat tggatataga aggacagaaa atgcaggaat
    22021 ggttgatata ttaaagtagc gaggtgagag aaataaaagc taaattttat agatcaggtg
    22081 atattgtaag agatattggc attgtttact tttatgaatt actaatggat ttaaaaaacg
    22141 aactagaaaa aaatgattat aagctaaaat ttacttgtga gttaaataga aattatttat
    22201 ctttagaatc tgcagaatat atagacccta agtatatatc tgattatata ttagaaaatc
    22261 gactttttaa ggtatttaaa aatgggaaaa aggaaacctt agaaaaaata tttagtaata
    22321 tagataattt aacgtatgta aattttattg aagaattaga taaatcttct gcaactgcta
    22381 aacaaaagga agatattaaa aaagatttta aaaacaggag gtttccatat gttagaaata
    22441 gtggtaagta tggattaaac acgagtttag aaaatatgga aacaaatgta aaaagaatag
    22501 tagatactgt aattaaatca aatatagaga tagatgaaat agataaactt gatttgacac
    22561 aatatgaaga aaaagatagt gtatgtaatg tttgcaatat aaataaaaca acaaaactag
    22621 atattgattt aagagaacga aaggactcta agtataactt tttatttaga ggtgcagaaa
    22681 aatcaggctt taaaagaagt ggacaagttg aaagtaatat ttgttttgag tgtgaatttt
    22741 tcaacttaat gtgtctttta tatataaatt taaaaagacc aatggttttt gcatatacga
    22801 atgatttaag agaattagca tttttaaatc ataagataat gcttaaaaga aaaatgtatt
    22861 cagataaatc attttataaa aaacttttgc atgaaaaaat aagttcaatt agactatata
    22921 gctttgatat tgatacgaat aaaggtataa ttctaaagtt tgattctatt atagagtata
    22981 aagaattatt aaaaaaaatt gaacttatag atattataga taattataat ttttcaagag
    23041 agccaggtaa tactagaaat ttgggtaaaa gaatgattga taatgggaat ttatcaaatt
    23101 taaaagaatt attacttgat aatataagtg ttttaaagca atcaactgga acttctcgag
    23161 aaatggattt tgtatctagc tcatataata tcggtttata tataaaattg tgttggattg
    23221 tagatggagg ggaggagtat aaaatgaaaa actatatgga aagtaattta atatattcaa
    23281 gagtaggtaa agacttattt aataaaatga cagatgaatc cagaagaaaa aatttttcta
    23341 tgaaagtgat tcaactactt aagtctaatg atagaactca gctatttcaa actataatgc
    23401 atgtgttagt ttctaatgga atcttaatag gagaagggtt tgtagaaggt ctaatgcaga
    23461 gttctgaatt agaattaaat acgaatgtag gtctatttat tcaagaaatc atgaaataaa
    23521 aaagggggaa aatttaaaat gaatgaaaaa agagtaaaag gagtaacatt aactatactg
    23581 acagaaagcc cagttccact ttcatatgac caagggtatg gaaattatac accaataaaa
    23641 aaagaacaat atagagaaaa aatacatgca aaaactagta tagccacaat aacgtatgat
    23701 ttaagaagaa tgctccacca agaatatggt tggaatttaa gtaatattgt ttttggaaaa
    23761 aaaggaaata tatatccatc tattaagaaa aatgtaggat gtgtagatga aaatggatta
    23821 gagactgatg tatttggtta tttaatacca cttaaagata aaggttctat aagtaaagct
    23881 tctccactta gaataatacc ttttaggtca ttaaatccat ataaaggttc aacacagcta
    23941 ataacaaata gaggcttttt aagtagtgaa tttggtagaa aatattatga tgagaaggaa
    24001 gaaaatgaag tacctagaga tgaaaacttt ccaacatcac aagcattagc aatagaagag
    24061 actttatcag attactacgt ttatacaata acattggagc ttgatagaat aggagtagta
    24121 gaagtagaag atggaaagtt acttttacca gaagaaagaa aatttatgag taaggagtta
    24181 agagaaaaag cagtaaaaga tatattagat gcaattactg tgtttacaag aaacataaaa
    24241 cattcatcag tactattaaa accacttgca gtaataggtg gagcttttga taaagttgta
    24301 ccattttttt gggatgatgt agattataat gctgatagtg gtgagataaa tttagaggga
    24361 gtcatagaaa ctatagaaag ttatagcctt aaagaaagta acacaatact tgctatcaat
    24421 gatagattaa aaatttctaa taaaaaggaa ttaaataagt ttaatttaag taaataccct
    24481 gtaaaagaga ttaaaaatct agcagataga ttggaaattg gagaagataa tatgtggtat
    24541 ttaaaagagt aggaggtttt ttgaattatg aaagtattac aatgtaaact taagcaacca
    24601 actgctcact atagagaccc aaaagtattt caaaatgaat atataagtac tttaaattta
    24661 ccatctaaga ccacaataat gggaatgata acgtatttat gtgatagaag attaaactct
    24721 gatatagata taggaataat tggaacacat catcatagag aactagaatt ttcaagagga
    24781 gaaaacattg atttttggaa tgaatatata aaaatgggaa aaggtaaaga taaagaaaag
    24841 tttttgctta aaggaaacta ctatgattac tataaagagc ataaagctca aaatagcata
    24901 ttaaactatg aagttcttaa agaagtagaa ctgactatat ttatttcttg caaggatgat
    24961 gaagaacttg aatttattag aaaaaagctt gaaagtccat gtaaatatgc aaatttagga
    25021 agaaaagaag attttgtaat accaagtgaa aaagggtgtt ttgtgaaaga agttaagtta
    25081 aaagaggtta tacctatgaa tactcgtgat gctataaaag aaaatataaa attaaagaat
    25141 acatatgtaa gagttgactt aagagataaa gagaatgtcg agactataat aaatcaaggt
    25201 gtattgattg cattgcctca taaatataaa gatttagaag caaatagaga tgatagagtt
    25261 tatgagtttt gccattatat atatgttgat aatgatggta tatatcctaa aaatattaag
    25321 gtaaatgtat gtattgatac aaaagaagtt tttacatggc tttaaattat tttgaaagat
    25381 aggtgtttat catgaaaaat atcatgcaaa aagaaatatt agctaagtca tacaatttaa
    25441 gaactaatca acaagaacaa acattagttg aacatattaa ggatttattt gaagtattag
    25501 aatctatatt agaattaaat ctatattcag ataaagatgt agagatacta aaaatatgtt
    25561 gtgctttgca cgatttagga aaaattaatt caattatgca acaaaaaatg gagattaata
    25621 ataaaatatc gtatagttgc tcagaagaag agaggaaaaa gttagagtct gacaaaaaat
    25681 ctttaaaaaa gatagcaaga cataatatat tttctggagc atttttaaaa gatatattag
    25741 aaaaaatgaa cttgtcagaa gaagataagt tatatatata taaaagtata atgttacatc
    25801 atggaaacta tgaagattat atgaggttaa gtattagtaa agttcaagaa gaaatttatg
    25861 agtatattga aaaaggcatt ttagaaaaag aagagtttaa tctgaaagat atagaaagtt
    25921 atataaatag cattttaaat gtagacttta aatttggtga agatgtattg gattatgatt
    25981 acatagataa attaagtgaa agtatgttta tagacagtga ttataacaat ggtcaaatag
    26041 atgattcagt tttaaattat agaaagttta agtatatttt atataaaggg atgctgaact
    26101 taatagacca tagtgcatca agtagacaga aaggtataaa attttataac gattttactg
    26161 atgaagaaat tgataatatg attttaaatg agatatataa gagtcagggt aatttggaaa
    26221 aaaataatat agagttcaat actatacaaa aaaggttgag agaactttct ggaagaaatg
    26281 ttctgactgt tgcatttaca ggttcaggaa aaacagctgc agattataga aagactttta
    26341 aaagaaaatt ctttttagtg ccaaataaaa tatcagcaga aagtttttat agaaaaaata
    26401 tttttcaaaa taaaaatgat ttagatacaa gaagtagtaa tgattacatt ggattacttc
    26461 atggagatat aaacttatat tcagagaatg aagataatgg agaacatgat tttgtattga
    26521 cattaaggga tatagatttg agtataaact tttgcaaacc ttgtgtaatt gcaacagttg
    26581 atcagttatt acttagtatg tttaaatttc cgggatatga aaaaatattc gcagccgtaa
    26641 aaaatgcatc tataactgtt gatgaagtac atttattgga accaaagatg tttttgattt
    26701 tgatatattt tatgcagttt gcatgcaagt acttagatgt tgattttcac ttaatgacag
    26761 ctacacttcc aaaatcatat aaagagcaaa tgataaacaa gggaattatt tttcaagaag
    26821 aaagtaatga aaatgttact gataaaggag aaatagtttt tattgagagc aacaaggaag
    26881 aagagatttg tgaaggtaaa gatgtaaagg tttcttttat aaaggaaaaa gaaatcaagt
    26941 caatagttga agatgcttta gaaaataaac aaaaaattct tataataaag aatactattg
    27001 attcagtcaa tagaacatat gagtttttaa aagaaaattt atctgataag tatggtggtg
    27061 tagatataga tgttttacat agtagattta agttcaaaga taaaaaagag aaatatagta
    27121 aaatattaaa tggagagggt gatatttgga tatcaactca atcagtagaa atatccttgg
    27181 atttggattt caatataata ataagtgatt tagcaactat ggatagctta atacaaagaa
    27241 tgggtagatg caatagaaat aataagtatg aatatggaaa cttttatatt ctacctagtg
    27301 aagataaaat ttatgacaac aaacttaaga gcacaactaa aaacatctta aaggacatat
    27361 taaaaactaa ctctattttt acaatgggaa ttagaaaaac tattttggat gattactatg
    27421 ataatagtgt agtcaaaaaa tattttgaag agaattttat aagttgtgat aaagaaatta
    27481 agaatattta tggtgtaaat aaagaaattt ttgatggatt ggatttaatt tttaattttg
    27541 aaccttacaa aaatattgtt gatagtaaga aggaagcagc taaaattttt agagatgttg
    27601 atgttagtta caaaattatt ctggaagaag atttttataa ggaagataga gagttacaac
    27661 aagattcgat tcaagtatca gggtttatat ttaatagatt atattattat gggttaataa
    27721 gcaaagtaga aggatacatg gtactaaaat ctagtagtaa gtttgagtat aatagtactg
    27781 taggcttaat tttgaagtga gcatatgttt aaatataata tacctttaga cattgaaaat
    27841 acaaaaagta ctactgtaag ctcactgtaa aaactatttt ttaaaaggaa aaataagttg
    27901 tgagatagag tgtttacaag tgtatataga taataaattt gaggtttaag attaactaag
    27961 tgggtttcag gtacatcgcg aaaataatta taagttggag attttgtatg tttaagatta
    28021 actaagtgga tttcaaatat tgatatagta gatataatag aagttttatt attaggttta
    28081 aaattaacta atcggatttt taaatattct gactattata taaggatatt attagattga
    28141 atttaaaacc aataatataa aacaacaaaa tattatattt atgatataat aaaagttatg
    28201 gaaaaacagg aatattacta tagagaagag aggtaaactt atgaataatt ggatagaagt
    28261 aactataaaa acaacaacag aagcagttga acctataacg aatatactat atgaacaagg
    28321 agctggagga gctgtaatag aagacccaaa agacttttta ttccaaaaga aaaatgaact
    28381 agactgggat tatgttgaag aagaagtatt taaaaagaat gaagaagatg atgttcttat
    28441 aaaaacttat gtttctgaag aaaaaaatgt gatggaattt gtcgaaataa taaagcaaaa
    28501 agttttagga ttaaaagatt ttggtataga tataggtgaa ggaagtgtat cattggacca
    28561 agtaaatgaa gctgattggg caaatgcatg gaaagcatac tacaaaccaa caaaagtagg
    28621 acaaagggta gtagtaaagc ctacatggga agattatgct atgcaagagg gggatttaat
    28681 catagagctt gaccctggaa tggcatttgg tacaggtaca catgagacta ctagtatgtg
    28741 cattagagaa ttagaaaagt atgtgaataa agattctaaa gtatttgata ttggatgtgg
    28801 tagtggaatc cttgcaatag cagcagctaa attaggagca aaagaagttg tagctgtaga
    28861 tttagatgaa gtggctgtaa aagttgcaaa agaaaatgtt cttgaaaata aagttgaaaa
    28921 aagtgtatct gtaatgcatg gtaatttaac tgatgtaata aaagataaag cggatgtaat
    28981 agttgcaaat ataatagctg atataataaa gatacttgca aaagatgttc aaaactttat
    29041 gaaagaagat gctatattca tatcttcagg tataattttg gataaagtag aagaagttaa
    29101 agaaagctta attgaaaatg gatttgaaat agttgaagtt caaaaattag gtgaatggtc
    29161 agcaatagtt tctaagctta aaaaataagg gatatggtga cgtataatgg atagattttt
    29221 tgttgaaaaa aataatataa atctacaaga taaaacttgc actatagaag gtgaagatgt
    29281 aaaacatatt tcaaaagttt taagatgcaa attaggtgag aaacttgaaa tatgtgacaa
    29341 gaataataat gagtatattt gtgagataat gaatatagat aagagtatag ttaatttaga
    29401 aattttagaa aaagttgata taaatagaga atcagaactt aaagtaaggc tataccaggg
    29461 acttccaaaa gctccaaaaa tggaaataat acttcaaaaa ttaactgaag tgggtgtaga
    29521 agaaataata ttagttcaaa ctaagagaag tgttgttaaa gttgatgaca aaaaagaaga
    29581 taaaaaattt gaacgttggg aaagaataat ttatgaagca gcaaagcaaa gcaaaagagg
    29641 taagatacca aaattaagag gtgttttaag ttttaaagaa gctttagaag atatgaaaaa
    29701 aaatagtgta aatatttgcc cttatgaaaa tgaaagaaca gtttcaataa aacatgcact
    29761 aaaaaaatgt gattctaata tagatagtgt tggaattttt ataggtccag aaggtggatt
    29821 ttctgaagaa gaaatagaac aaatacaaaa aaataattgt aatgttgtat cattggggcc
    29881 tagaatatta agaactgaga cagcttctgt tgtagcatca acaatagctt tgtatgaact
    29941 aagtgattta ggaggagaaa aataaattga aaaaagtagc tttttataca cttgggtgta
    30001 aagttaatca atatgaaaca gaagctatgc tagaactatt tgagaaagat ggttatgaac
    30061 aagtcaatag tgaagagtat gctgatgttt atgttataaa tacatgtaca gttactcata
    30121 tgagtgatag aaaatctcgt caatatataa gacgagttaa aaagaagaat ccagatgcaa
    30181 taatagccgt tgtaggatgt tattctcagg tatctccgga agaaattttg gatatagaag
    30241 aagtaaattt agtcatgggt acaaatgata gaagaaagat tgttgaagaa attaaaaaga
    30301 taaattcaag taagaaagtt agtacagttg atgacataat gaaagttaaa gcttttgaag
    30361 aaatagaaat cagccaaact aatggaaaga ctagagcatt tatgaaaata caagatggat
    30421 gtgacagatt ttgtacttat tgtatcattc cttatgcccg aggacgcgtt agaagtagag
    30481 atattgatag tatagtcgat gaagttaaaa aattggctaa taatggctat aaagaggttg
    30541 tattaacagg aatacatgtt gcctcttatg gaaaagattt aaaggatagg gatattaaac
    30601 ttcttgatgt aataaaacaa ataaaccaga tagaaaaaat agaaagaatt aggcttagtt
    30661 ctgttgaacc aattttattt acagatgaat ttgtaaatga agttttaaag atggataaag
    30721 tttgtcctca ttaccattta tcacttcaaa gtggatgtga tgaaactcta aaaagaatga
    30781 atagacgata cacaacttta gagtacaaaa ctattgtaga tagattgaga agtaaaatgc
    30841 cagatgtggc aataactaca gatgtaatag taggttttcc tggagagaca aatgaagagt
    30901 ttaaaaaaac gtacgagttt ttaaaagaaa tagagctttc acaaatgcat atttttaaat
    30961 attcaccaag aaaaggaact ccagctgcaa ctatggaaaa tcaagttgac cctcaaatga
    31021 aacactttag aagtgaacaa cttttaaatt taagtaaagt aaattttaat aaatttgcaa
    31081 ctaaatttat cggccgtgag ttggatgttt tatttgaaca aaatatagaa ggcaataaat
    31141 atgagggatt aacttcaaat tatataagag ttgttgttga aagtgacaaa aatatacaag
    31201 gtcagatatt aaaagtgaaa ataaatgatg taaaagatga atatgtagaa ggaattttgc
    31261 tataacatgt agaaattata atatttataa gataggaggt ggaaagaatg gattgtattt
    31321 tttgtaaaat agcgaacgga gaaatacctt caactaaagt ttatgaagat gatagagtct
    31381 tagcttttaa tgacttgaac ccagttgctc catatcatat attagttgta cctaaaaaac
    31441 attatgatag tttaattgat attcctgata aagagatgga cattgtgtca catattcatg
    31501 ttgttattaa taaaatagct aaagagaaag gttttgacca aactggattc agagtcataa
    31561 ataattgtgg aagtgatgga gggcaagaag ttaaacattt gcactatcat attttagctg
    31621 gtaaaaagct tcctaattac gaagcgggac aaaactaaac taaattattg aaaaattgag
    31681 ttccttcttg ataaaaatac ttgataaaag taaaaattta gtttataata aacaaagtgt
    31741 taggttgtaa tgcatattat gcaaaaagaa catagttaca gtcccagcat aattgcgcta
    31801 ttggagggag ggaaaaagaa atgtcagaag taagagttag agagaatgaa acattagaca
    31861 gcgctttaag aagattcaag cgtcaatgtg caatgtctgg catcatgtca gaagtgagaa
    31921 aaagagagca ctatgataag ccaagcgtta agcgtaagaa aaaagctgaa gcagcaagaa
    31981 gaaaaaacgc taagaaatag tagtaaataa gaataaagag gtgaagggga tgtcccttaa
    32041 gcaaaagtta caagaggatt tgaaatcttc gatgaaaaac aaagatacag tgagaaaatc
    32101 tgtagtaact ttaataagag cttctataaa gcagtatgag gttgataata gggtcgagct
    32161 tgatgaagat ggaattatcg atgtaatagc aaaacaatta aagcaacgta gagatgcatt
    32221 agttgaattt gaaaaagctg gaagagaaga cttaataaaa gaaacagaag gtgaaattga
    32281 agttttaaaa gagtacctac ctcaacaatt aagcgaagaa gagttagaag aaattgtaaa
    32341 atctactata tccgaagtag gggcaacttc tatgaaggat atgggaaaaa taatgtcagt
    32401 tatccaacca aaagttaaag gaagagctga cggaaaactt ataaataagt tggtaaaaca
    32461 aaacttacaa tagattgata tagcctagag agaaatctct aggcttttta tagtttttgg
    32521 gaataacgag tcatatttga gcatatctat aatcaaagaa aattttgaaa gaaggggaaa
    32581 aatttgatta tatcagtaaa agagattagg aattttttga taaacatatt tataccacta
    32641 ataataggtt acttaagtag catcttaaca atgattatca gtggaacaga tatttcaacc
    32701 tattatttac aactcacaaa gccaagcttt gcaccaccag cttttatctt tcctatagtt
    32761 tggactatat tgtacatatt gatgggtata tcttcatacc taatattaag aaagggttac
    32821 aacttaccta aagtaagaga tgcaattttt tattatggat tacaacttgt attaaacttt
    32881 atttggagta tactattttt tggatttgga cttagattta cagctttgat agatataata
    32941 ataatgattt tggtttctat aataatgatt tcgaagtttt ctaaaataga taagagatct
    33001 ggatacataa atttgattta tttaatgtgg cttgtttatg caggattttt gaactatttt
    33061 atatggttta ttaataaata gaatgtaaag attttgtaaa ttaccctgta cttttaataa
    33121 tatcataaca aacgaaaaat aaatagacta ttaaaaataa tcattttcat atacaataat
    33181 attaatttaa atacaataaa tttataaaaa ttgtatttaa agattaacat attaaagtat
    33241 tagaattgga aaggcttgaa gggagaggtt tttatgcaaa taatagtatc tgggagacaa
    33301 atgaaattaa ctgacggaat aaaaggatac gtagatggaa aattaagtag gttagaaaaa
    33361 taccttgatc ctgaatcaga agtgaaagta acagtaagtg cgaaaaagga tagacaaaaa
    33421 gtggaggtta caataattcc tattaacggt caaataataa gagcagaaga tgtagaagat
    33481 gacttatatg cagcaattga tattgtttgt gacaaattaa gtagacaagt tgtcaaatat
    33541 aaaactaaag ttaaagacaa agttcaaaat aataagagca taagatttga aaatcttgac
    33601 tttatagata atagcagtga atttgatgat tatgattatg atgatgaaga agatgaaaat
    33661 atagtaatag aaagaagaaa gaaatttaat gtaaaaccta tgagttctga ggaggcgata
    33721 cttcaaatgg aactagtagg acacaatttc tatatgttta gaaaccaaga taattttgaa
    33781 ataaacatag tgtataagcg taaagctggt ggatatggtt taatagaaca agattaataa
    33841 aaattgtata cgtttagatt ttatataaga aaaaataaaa aggtaatcat gttgattttt
    33901 tatcaatatg attacctttt tcttgtttaa ataatatatt agatattagg tatttattat
    33961 ataaaaattt taaaactaat ctgttacaac atcaataaag tgcataaata cctatttcaa
    34021 gcataaaatt aattaagggg gtaatgctta tgttagaaat atccactgat ttacaagtaa
    34081 atcaaccagt tattactgtt acaagtaata cgtttattag tatagaaaat tatctatcaa
    34141 tattagaata tgaagttgat ttaataagaa taaagactaa agttaaaaca attaaaatat
    34201 ctggggataa actttcactt aagtatatta ctgatagtga gataggtata aaagggatta
    34261 tctacaatgt tgagtatgtt gattaggagg agatacactt gataagtttt ataaggggat
    34321 actatgttat agttgtggaa ggagtaggac tcgaacaatt tttaaatcat cttataagaa
    34381 atgggataag tgtttacaat gtaactagaa taaaaaatac taaaatggag tttcatatag
    34441 atagacaaga tataaaagac tttaagaatg tttatagagg aagtaagttt gatataaagg
    34501 taaagcaaaa aactggggtc ccttttatta tcaaaagagt ttataagcac aaaggtatgt
    34561 ggatttgtgc tcttgtttct ttatttttat tgatgtctac atcacagttt gtgacagatg
    34621 tatatataca atctcctgaa ggtataaaaa aagaagctct tagaaatgag ctttataagg
    34681 ttggagtaag gccaggagtc tataagaaga gtattgatag aaaagaagtt agagaccata
    34741 tgatgtcaaa atttaatgat gtggcatatt tatctatcaa tgtaaaagga acaaatatat
    34801 ttgttactgt tacaaaaaag gcagaatccc taaaatctac agaccaatca aattattgta
    34861 atgtaattgc tttaaaaaat ggtataatag aaaaggtaat ccctagaagt ggtaaatcag
    34921 ttattaaatc tggagacata gtccaaaagg gagatgtctt attaaatgga gctaatacca
    34981 agtcaatacc agaagtatgg gcaagtactt tttatgagtc cacaaaaaag gcaagttatg
    35041 tagatactgt aaataaaaaa actggagaaa agaaaaatat atatacacta agtttttatg
    35101 ataaagagtt tacaatgaga aaaaatataa aatataaaga ttatgttgtt gaaaataaag
    35161 aaaaaaaact atcaatagga aactatactt ttccaataaa gataaaaacg agtacttttt
    35221 atgaaacaaa aaaggttgaa gtaaaaagga ataaagaaga gttaaaaaaa gaattatctg
    35281 aaaaagcttt aaaagagtta gaatacataa tcccagcttc ggctagaata atagatgtaa
    35341 aacataactt taaggttaat aaaaatatgt tagaatattt aataacagtt caaactagtg
    35401 aaaatattgc taaaatttat cctcttagta agtcagaagc agaaaggttt ataaaggaag
    35461 aaagtaaacc agatgaaggt gaagaggaag ttccttccaa tccagaaaaa agaccattaa
    35521 atgatataag aaatgagttt gatgaggata ataaggataa taaagaccaa aacaattctg
    35581 atgaaaataa tagtaatcaa aatagtaata atagtcaaaa taataatagc aataacaact
    35641 aataaaatta aacttaaaat cttaggagga ctaattcaat tgataataca aaagaaattt
    35701 acaatagcag atggtaactt tgaaagagag ttatttggca actttgatga aaatgtaaaa
    35761 ttaatagaga agactttaaa tatagatgta atacttagag aaggcaatat aattttaatt
    35821 ggagaagaaa aaaatgtaga ttctgcatta aaacttatga atgaattaca ccaaactgta
    35881 tcaaatggta agcatttaga taagcagagt atttcttatt cattatctct tttgcttgaa
    35941 ggaagtgaac aaaaaataaa agaacttgag gggacaatcg tcataactca aaggggaaaa
    36001 gctgttcaac ctaaaacttt aggtcaaaaa gaatatataa aacttataga aaataatgat
    36061 ataacttttg gtgttggacc tgctggaaca ggaaagactt atcttgcggt tgctatggca
    36121 gtaaaagctt ttaaaagaga tgaagttagt agaattatac ttacaagacc agctgtggaa
    36181 gcaggagaga gtctaggttt tttaccagga gatttaaagg acaaagttga cccatattta
    36241 agaccactat atgatgcctt atttgaaatg ttgggtgctg ataaatttaa taagtattta
    36301 gaaagaggta ctattgaggt tgcaccactt gcatttatga gaggtagaac acttgataat
    36361 tcatttataa tattggatga agctcaaaat actacttctg aacaaatgaa aatgttttta
    36421 actagacttg gatttggctc taaagctgta gttacaggag atgtaactca aacagattta
    36481 ccacaaaata agaagagtgg tctaatacaa gctacagaga ttcttaaagg tgttccaggt
    36541 attgggtcta taatgctaac agatagagat gttgtaagac atgagttggt acagagaata
    36601 ataagagctt atgagaagca cgacaaaaga gaagaattta aaaaagaaga acgtaaaaaa
    36661 gcgaaaatgc aggaaaagac tttcaaaaga aagtaaaagt gtagtaatat agatatatag
    36721 cattattaat gaataaaaag taggtgtagt gaatggattt gatactagat gatagacaag
    36781 ataaattaga agtaagtgag gagttaattg aaaagataaa ggatataatc atagaatgtt
    36841 tagattatga aggatatgat gataattatg aagtgagtct ttcttttgta gataataaag
    36901 agattcatga attaaataga gaatatagag gagttgatag agtgactgat gttttatcat
    36961 ttcctctttt aagtgatgat tttgaagatg ttgagttaga agaagaatcc ctaggtgaca
    37021 tagtagtatc tcttgaaaga gccttagaac agagtataga gtataaccat agttttgaaa
    37081 gagaagtatg ttttttaata tgtcatagta tgtttcatct tttaggttat gaccacgata
    37141 cagatgaaaa tacaaaagaa atgagagaaa aagaagaaca catattaaat aagttaaaca
    37201 taacaaggga gtagttttta tgaaaccaga aaaaactcgt caaggtataa ttaaagcatt
    37261 taatgcagct atagaaggga tattgtatac atttaaattt gaaagaaata tgaaaataca
    37321 ttatttaggt tcagttgctg ttcttataat aagtttgttt tttaactttt caaaattaga
    37381 aatgataatg ttacttatgt ccatatgttt agttgtagtt gctgaaatgt ttaatacagc
    37441 tatagaaaaa gctgttgatt tagtaactga tgaatatcat gttcttgcta agatagcaaa
    37501 agatgttgca gctggaggcg ttctagtggc agcactaaat tcagtagtag ttggatatat
    37561 tttgttttat gataaattga cagatatatc tggaatatta atttataaaa taagagaatc
    37621 agaattacat ataaccttga tatgtatatt attggttctt atagctgttg tagttgtaaa
    37681 agcattgact tctacaggta ctcctctaaa aggaggaatg ccaagtggtc atgctgcctt
    37741 agcatttgct atagcaactg caattacact tatgactgaa agagttgttg cctctactct
    37801 tgcatatata atggctgtat tagttgccca aagtagaatt gaaggaaaaa tacatacatt
    37861 ttgggagact attgctggag ctttgttagg tgtgttgata gctatattag tatttcagct
    37921 tggaatgttc tataattaaa ataaatgtac ataaaatatt taaaataaaa caaatctgtg
    37981 ctaattaaat ttttatgata taatgtagag tattaaataa aaataacttt taattattgt
    38041 atacaggagg agttgttgtg gataacatag aattgttaag gttggcagaa gatgccagac
    38101 aacattctta tgctccttac tcaggtttta gagtaggtgc agctctttta actaagagtg
    38161 gtaaagttta tactggatgt aatatagaat gtgcatcatt aggtggaaca aattgtgcag
    38221 aaagaacagc tatttttaaa gcaatatcag agggtgataa ggatatctat aaaatagcta
    38281 tagcaagtga taactcagaa aataatgagc aaacatatcc atgtggaata tgcaggcagg
    38341 taattataga gtttggttct gatataaaaa taataacggg atatactaaa ggagaggtgt
    38401 ttgagcatag tataaaggat ttgttgccaa actatttctc tggaaatgat ttaaaataaa
    38461 tttaaaagta taaataaatt taagtaatta aaacaagaag ataaaaagtt aggagacagt
    38521 taatgttcaa atcaggtttt gtcagtatag tgggtagacc aaatgtagga aaatctactt
    38581 taatgaataa tgtagttgga gaaaaaatag ctataatgag tgataagcct caaactacaa
    38641 gaaatacaat acaagcagta tatacagatg aagaaatgca gatagtattc ttagatacac
    38701 caggtattca caaaccaaaa aataaattag gtgaatttat ggtaaaggca gcaacagaag
    38761 cttttaaaaa tgtagattta atattatttg tagttgatga ttctaaaaaa ataggtcctg
    38821 gagatagaaa gataatagag gatttaagaa gtgttaagac accaataatt ttagttgtaa
    38881 acaaaataga ccaactagac caaaaagatg agctatttga tataatcaaa atgtatgata
    38941 gagaaggtat ttttaaagaa atagtaccaa tatcagcatt aaaaggaaag aatacggata
    39001 ccttaataaa agttatacaa aattatttag aagaagggcc aaagtatttt ccagattata
    39061 tgattacaga ccagccagaa agagtattaa tagcagaact tataagagaa aaagttcttc
    39121 actatcttaa tgatgaaata cctcatggtg tagctgttga aattgaaaaa atgaaagcga
    39181 gaaatgataa ggaaatagta gatgtatctg ctgtaatata ctgtgaaaga gattcacata
    39241 agggaataat aataggaaaa aatggtagga aactaaaagg aataggtaag tcagcaagac
    39301 aagatataga attgctttta ggttcacaaa taaatcttca attatgggtt aaagtcaagg
    39361 aaaattggag aaatttacaa aattatataa ataattttgg atataatgat aaataaaggt
    39421 gggttatatg gataggaaac tggatgcttc ccaagattta ttatatgaag taaaatcatt
    39481 aattgataat aataaagtat tggagttaag agaattaata gaggaatatc atataattga
    39541 tatattcgat ataatggaaa atttagagga agacatgaaa attcaattat ttgaagtact
    39601 tcctttagat atggcatctt ctatattaga agaaggtagt gtagagtttt ttattagtat
    39661 tttatctaaa ctggatgtag aacactcgaa aaatatactt gaacttatgt ctcttggaga
    39721 tatggcagac aagttgagtg aacttgaaga agaagaaaga gaacacataa taaatctttt
    39781 gaatcaagag aatgccgatt atgtaaaaga gttactattc tatgatgaag attctgctgg
    39841 tggaacaatg accactggat atatttctat aaataaagat atgacagctc ttgaggctat
    39901 agaccatatg agagaagaag ctgaagaagc tgaaactatt tattatatat atgtggttga
    39961 tgatgaagaa aaactagttg gagtattgtc attaagagaa ttaataatag ctagagacgc
    40021 aaatatagta gaagatttga tgagtgaaaa tataatctct gtttatgtag atgaagatag
    40081 agaagaagca gtaagattag tatctaaata caatttaata gcaattccag tagtagacag
    40141 acaagaaaaa ttaaaaggaa taataacagt agatgatata attgatgtaa tggaagaaga
    40201 agcaactgaa gatatgtata aatttgcagg aagttcagaa catgaaagag aagttgcgga
    40261 aaaagaaaac cctacattga gagagcaaat aatatcagca cttagaggac gattaccttg
    40321 gttaattatt accttagttg gagggttgtt ggcttcgtta atactttcta acttagacta
    40381 tataatgaat cctgtatatg ctccattagt attttttata cctgtagtaa ttggtatggg
    40441 tggaaatatt ggaactcaat catcatctgt tacagtaata acattgtcta ataaggattt
    40501 aaattttagc aatgtggtta gagaaggtat tgtaggtatt attacaggtc ttttgtgtag
    40561 tataataaca ggtattgtaa tttactttgt tatgagaaag ttagacattg tattaattgt
    40621 ttcaatatca ttatttataa atatggtttt aggtgcaaca ataggtgcgt ttatgcctgt
    40681 tttattaaaa aaaatggatg cagacccatc tacagtttca tcaccaatta tatctacagc
    40741 tcttgatata actggtatag cagtttactt tataataaca acggcattat tgtcaaaaat
    40801 tgtttaataa ctttatgtat gatttatcct cttcttacta aaaatataag tataaatgta
    40861 agaagggggt tttttaaaaa tgaacaatat cagttgggaa gtttttaaaa aaacaggtag
    40921 tatagatgca tatttatatt tctgtgattg caaaaacata catgaagaaa ttgaagaagt
    40981 aagggagaaa aaagaagacg atgataatcc tgaacaccca ggggatagta cttaaagcta
    41041 taaggtataa ggaaagtgat ataatactta cactatttac aagaaagctt ggaaaagtat
    41101 ctgcaatagc aaaaggagct aaaaaaaata agagctcttt gctttcatca tcacagttat
    41161 tttcttatag caattttacc ttaaaaaaac aaggtaatat gtacaaggtt actcaaagtg
    41221 aaataataaa aagtttttat aatatatcct atgatataga ggcattttct tatgctacat
    41281 atattacaaa attagttgaa aattctatac ttgaaaatca gactaataat aggctattta
    41341 tattacttgc gcagactctg tatttgtata cacaggataa tacagacaat aggtttataa
    41401 cagcagcatt cgagttaaaa tttttagact atataggatt taaacctata gtaaataaat
    41461 gcattaattg tgaatgtaaa attttacaaa attctgtgtt taatatctac gaaggtggta
    41521 tactatgtag taagtgtagt aaactttttg ataataatat aaaattagac ttaacaacta
    41581 taagtcttat ggaatatgta ttaagaaatg atattttaac atgtagtaaa gctaaagtat
    41641 caaaatatat aacacatgaa cttgagaata ttctagacaa gtatttgaaa gtttatgttg
    41701 ataatattaa ttttaaatca ttacacgtat tgcaaagtgt aaaaaataat aagggagtgg
    41761 ataatgatga gtagtaatat aactattgaa atggttgatt cagtctttga aagagttcca
    41821 ggagcaactt atgcagaagc aaaagaagca ttagtaatgt gcaatggaga tgtaattgag
    41881 gcagtaatat atttagaaaa taaaaaaaat acttgtaatt gtaaagcaaa atctgctaaa
    41941 gaaactatgg aagaagtatt tggcaaagat ggagaaaata taaagtttca attaaaagag
    42001 cttcttagaa aaagtagtgt agtgcgaata atagttgaaa aagatggaaa aactatgatg
    42061 aacattcctt taacagttgg agttgttgga ttagcttttg taccattagc tactttagtt
    42121 ggattatcag ctgctgttat aagtaaatat cgtataaaag ttcaaaatga agaagatgga
    42181 gaaattgtag atttaggtga attgaatgaa gaaaaacttc acgttttgaa gaatatgata
    42241 actaatgcag caaaagatgt gaaggatgta gtagttgaca aaaaagaaga tgacaaagat
    42301 gtaactgctg accttatgaa agaagaatct gaaaagaata aagaagacga agacgaaaat
    42361 aaatagatat aaagtgactt ttaaaaaatg aagagaagat aattataaga attatcacta
    42421 acattgacaa attttataga atttgatatt ctaaaagttg tagaagaact attaaaatag
    42481 cctttcgtgt agacgagagg ctttattgtt ataggaggga tttttatgaa ttttcaagag
    42541 atgatactgg cgttgcaaaa atactggtca aagcaaggtt gtatcatgat gcagccttat
    42601 gatattgaaa aaggtgcagg aactatgaat cctaatactt ttttaagatc attaggacct
    42661 gagccttggc aagtatgcta tgtagagcct tcaagaagac cagcagatgg aagatatggt
    42721 gaaaacccaa acagactata tcaacatcat caattccaag ttatactaaa accttctcca
    42781 gataatatac aagaattata tttggaaagt ttaaaagaga ttggaattga cccaagtgag
    42841 catgacataa gatttgttga agataactgg gaagctgcta cagttggtgc ttggggactt
    42901 ggatgggaag tttggctaga tggtatggaa ataactcaat ttacgtattt tcaacaagta
    42961 ggtaatattg aatgtgagct tgagacagga gaaattacat atggattaga aagattagct
    43021 atgtacattc aagaagttga tagtgtttat gatttaaaat ggaatgataa aataacttat
    43081 ggtgaagtat ttaataaagc tgagtatgaa aactcaatgt acgcatttga attatgtgat
    43141 gcagatatgt tgtttaatct gtttgatata tatgaaaaag aagcattaag acttatggaa
    43201 aatggccttg taataccttc atatgactat gtgttaaaat gctctcatgc atttaataca
    43261 ttggatgcaa gaggtgcaat aggagttagt caaagagctt catttatagg tagagttaga
    43321 aatatggcaa agactgttgc agaaactttt gtaaaacaaa gagaagaaat gggattccct
    43381 cttttaaagg acggtgacaa ataatgaaaa attatctttt atttgaaatc ggtgttgaag
    43441 aaatgccatc aagatttgta ggcagtacac tagaacaatt aaaaaacaat cttagtaaat
    43501 tatttgatga aaatagaata gcttttgata aaataaatgc gtatgggaca ccaagaagac
    43561 ttactttagt agtcgaagga ttgagtgata gacaaaatga tttagaagag gaaataaagg
    43621 gtcctgctaa gaaaattgct gttgatgcag aaaataattt aacaaaacca gcattaggat
    43681 ttataaaaag caaaggatta agcgaatctg acatatattt taaacaagtt ggaaaagaag
    43741 aatatatatt tgcgactata aagcaagatg gaaaagaaac ttcagaagta ctaaaagaca
    43801 tattaccaga gactataaaa tctgtagtat tcccaaaagc tatgcgttgg ggaggaaaaa
    43861 atcttaaatt tgcaagacct ataagatgga tagttgcatt gttaaatgat aaagtattag
    43921 agtttgattt agaaggaata atatcttcta atgtaacaaa aggtcataga tttttaggtg
    43981 aaagtacaat agaagttgat tctatagaag attattttga aaaattagaa aaaaattata
    44041 tagttttaga ccaaaacaag agaaaagaaa tgataagaaa acaatgtata gaagtggcaa
    44101 attctttagg tggagaagtt gaatttgatg aagatttact tgaagaggta acttacttag
    44161 ttgaatatcc aactgctttt tatggagaat ttgatgtgga ttatataaaa ctaccaaaag
    44221 aagttgttat aactcctatg aaacaacatc aaagatattt tccagtactt aaagaaggaa
    44281 aactgttacc taactttata gctgttagaa atggtgattc ttacagaata gacaatgtta
    44341 aagctggaaa tgaaaaagtt ttagaagcaa gacttgcaga tgctttattt ttctatagag
    44401 aagatactaa gagaaactta gaaagctaca tagaaaaatt aaaaactgtt gtattccaag
    44461 taaaactagg aactatttac gacaaaactt taagacttga aaaattaagt gctgatatac
    44521 ttgatagttt aggtttagta gatgaaaagg cagatactat aagggctgct aaattatcta
    44581 aggcagattt agttactgga atggtaggag aatttgatga attacaaggt tttatgggaa
    44641 aagaatacgc aaaagttgga ggagaaaatg atgttgtaag tgaagctatt tttgaacatt
    44701 atcttccaag atttgcagga gatatacttc ctaagactaa tgcaggagtt gccttatcaa
    44761 tagctgataa gttggattca atagcaggtt tctttgctat aggaatacaa ccaacaggtt
    44821 ctcaagaccc atatgcttta agaagacaag ctttaggtat acttagtata cttatggaca
    44881 gaaaagttgc tgtggatata aaagtactta ttgaacatgc tttagacaat tattctgaat
    44941 tagattttaa taaagaagaa gttgttgagc aaataatgaa tttcttcaat gagagagtaa
    45001 aaaatctatt taaagattta ggaataagat atgatgttgt tgatgctgtt ttaagttctg
    45061 atataaatga tgtttctgat atgcatatga gagctttaga gcttaataat tggctagaaa
    45121 aagatgaatt agtggagatg cttactgcat ttaatagagt ttctactcta gctcaaaaag
    45181 cagaaagtga taaaattgat gaatctttat taaaagaaga tgctgaaatt aaactttaca
    45241 atgaatttaa acaagtaaag gtaaaagtta atgaattatt aaaggataag aaatatggta
    45301 ttgctttaga tgaatttgca tctttaagac cagtagttga taatttattt gatacagtta
    45361 tggttatgga taatgatgaa gctattaaga acaatagact tgctttactt aaacaagtat
    45421 atgatactat gctaggaatt tgtgatttat ctaaaattgt ttataaataa aatttaaact
    45481 atttataaga aataaggctg tctcaaaata gagatagtct tatttttcat tgttgagatg
    45541 atgaaaatat atttgatttc atagaaaaag agtgtctcat aaactaaaaa gttcattttg
    45601 agacacccat gctttttgta agtaaatata ataaattata ttttatactt atcagttatt
    45661 ttgtttaaaa tattatcatt tgaatttacc cattcataaa aattgcctga tgatgcgtcc
    45721 gatattttat aaagcatttc aggaatatca ctagctttaa aatctccaag tgattttccc
    45781 aatctagtct tacctatttc aaaactgcct ccaacaaata attcaaatgc atctgtagat
    45841 ataccatcaa cattcttctt tttaccacat aaacctatgc taccaacttg atgaacacca
    45901 catgaattta aacatcctga tatatataac ttaggtgctt tatttaaaat ttctttatta
    45961 ttttgtaatc taaaataatc tatgatttca tgtaacattt tctgactatt ttgtatgccc
    46021 atttgacaaa ttggaacacc tatgcaagat atactttgtt ctaattgaga attacaacta
    46081 atagttttac ttatttctaa tattcttttt gcttcatttc catttaaatt taaaatatat
    46141 aatccctctg tcattcctag tctaatcata ggattttcaa tattatctaa ttctttaagt
    46201 aaagtactta aatcctttgt taacaaaata cctccaactg gatgtatata tacagtgtat
    46261 aaaccatcct gtttttgagg tattagtcta gaatcctgta tatctatttt aaccccaggt
    46321 ttagagtaat ctatttcttc aatattgagt tcaagactac cattttcttt ttctaattta
    46381 tagtattctt taaatttttc taagaataat ttttcaccaa gtgattcgac catatatcta
    46441 accctagctc tgtttttatt ttcataattt ccataatcca taaagaattt aatcatacct
    46501 tcaacacaat ataaggcatc tttcggtttt ataagttcat caagttctaa agcaactttg
    46561 ggttgtttac ctagacctcc acctaagaaa actctgaaat atggttcatc atttttcaaa
    46621 gtagcaacaa atcctaagtc ttgtatacta caatgagcag tatctgtgta acaattagaa
    46681 taagaaactt ttaattttct aggtaaatgg tatgtagtag catttttgat aaaatattta
    46741 tcagtagcaa ctgcatatgg agtaacatca aagacttcac tagggtcaac tccagataat
    46801 ggagataatc cgacatttct aggataatta ccaccaccac ctctagtgaa tatatcattt
    46861 tttattcctt cttccatgat atcaacaatt gaatttatag ataaatcgtg taattgtata
    46921 gcttgtctag ttgttaagtg tatcttatct aagttgtgtt taatagctat ttcatagatt
    46981 ttattaagtt gatactgaga taaaactcca gcagatactc taagtcttat cataaaagat
    47041 tttttgtctc tttgagcata aacaccatat ccaccagaaa atcccttata ttgcatttta
    47101 tttatttctc catttaaaaa ttttgaagaa tattctttaa aattaggtag ttcatttaac
    47161 aatatctttt taaaattatc cattatttca cctcattgtt agtcttgtat aaaaatatat
    47221 ctattgtata atagatatat atattattat ctagtcagga taaaaatata acagaataat
    47281 tctattaaaa gtaagaataa taaggacata gtgagtattt tgtttttatt ttacaaatta
    47341 agtataatat ttttgtttta taaaatataa ttaagataaa tagataattt tgtaatttta
    47401 atatataata tttagtagag tgtgggagta gcacttaata tttgaaggag gagtttttgt
    47461 atatgggaga aataataaat gcattagata gagccctaga cataattttg ctgttatatc
    47521 atgaaaagag agaaatggga ataactgaaa taagcaaggc tatgggagta tataaaagta
    47581 ctgtacatag aactttggtt actcttgaaa acaaaggttt tgttatacaa aatgcagaga
    47641 atggtaagta ttggctagga ataaatttat atgcaatagg tatggttgtg ggagaaaaaa
    47701 tgtcattgac tgaaatagta aaaccatata cgaaaaaatt aaatcaagaa tttaacgaag
    47761 tagttaatgt atctatatta gaagaaagag ctcaagattc tccaagaagt atcataatac
    47821 ataaagagta tggttcaaat caattacttt cagtaaatcc atccgttggt tcaagtagtg
    47881 aatgttattg ttcagcagtt ggtaaatgtc ttatggcatt taatgattct atagattttg
    47941 aaaaatatag aaaaacacca atacacaagt atacagaaca tacaattgat aattgggatg
    48001 atatgatgct ttttttagca aaaataaaag aacaaggata tgctatagat gatgaagaac
    48061 tagaacatgg actaacttgt attggagcac ctatcttaga taaaaataat aaagctattg
    48121 cagccataag tttgtcaggt ccaactataa gaatgagaga aggggacttt gagtataaga
    48181 ttaaaagagt tatagaaact gcaaaaagta tatctgaact ttttagatga tttttaatct
    48241 cgataataaa aataggctat atttaaattg ttatttgtaa atatcagttt aaatgtagcc
    48301 tatttttctt taatctaaaa ttaaatattc ttaaaattta aaatgttatt gaaatatata
    48361 agcgaatgtg ttatctttta attaagcaat aaaaatagaa caaggttcta tactatagaa
    48421 caaaattgat gcatatatag tatttataca aaatataaat attatatatt taaaattaag
    48481 taaaaatatt acataaaggg gagatttgag aaatggaaaa agtcaagttg agtgttgaaa
    48541 gatgtaaggg atgctatctt tgtattgaag cttgtaaaaa agatgcaata agtctttctg
    48601 actacacaaa taaaaaaggc tttgtaacta ttaaagtcga tgaagaaaaa tgtattcaat
    48661 gtggcgcttg ttatacaatg tgccctgatt tagtatttga gatattataa attattttta
    48721 ttagagggga gaaaaggtat gcaaagttta ttaattaaag gaaatgaagc tttagcagaa
    48781 ggtgctatac gtggtggctg cagattttat gcaggttatc caataacgcc acaatcagaa
    48841 atattagaaa tgatggcatg gagacaaaaa gaagttggag gagtatttat ccaaggtgaa
    48901 tcagaaatag ccagtgtaaa tatggttatg ggagcaagtg cacttggagc aagagctatg
    48961 acttcttcat ctggtccagg attttcatta aaacaagaag gtattgcata ttgggtatca
    49021 gctggtttgc cagcagttgt agcatgtgtg caaagatatg gtataggtga tggatttata
    49081 ggagctggtc aagataatta ttggcaagca gtaaaaggcg gtggaaatgg agattatcat
    49141 ttaatcgtgt tagcacctaa tagtgttcaa gaaaatatgg atatggcata tgaatctttt
    49201 gaattggctg aaaaatatat gcatccagta cttatacttt ctgatgctac aataggtcaa
    49261 atggtagaac cttgtatagc accaccaatg aaagaatacg atatggataa agctccatgg
    49321 gctgcaaaag gaacacctgt aggagaaaaa aataaagtaa taactgatat aacatatttt
    49381 gaagaccaag atacatggca aaaagggtat atagaaaaaa tgagaaaagt tcaagaagat
    49441 gaacaaagat gggaagagat agaaatagaa gatgctgaag tggtatttgt ggcatacggt
    49501 atttcatcaa gaatagcaga aggtgcagtt agagatgcta gagcaaaagg aatgaaatta
    49561 ggtctaataa gaccaaaaac attgtggcct tacccaagaa aagcatttaa gaaattaagt
    49621 gattcattga agggacttgt aacagttgaa atgaacatga tggcacagat gaaggaagat
    49681 gtaattatag catgtgataa tagatttcca gtatattcag tagcaacagc tcaatatatg
    49741 gcagaagtaa atcaattaat agattttgct caacagatta tagatggaaa agctaagcaa
    49801 gaggaggtat tttaattatg gctaaaaaag caccagcaat aatgatgtgt gaaaatatgt
    49861 tctgtggagg ctgtggacac tctatagtcg atagaattat tggagaagta ttaacagaaa
    49921 tggatatatg tcaagataca attatatgtt cagatatagg ttgtaatcat atgtttcaat
    49981 ttagtatgaa tgtagatgtt gtcgttccac ctcatggtaa aatgggagca gcaatggcag
    50041 ctatgaaaag agttagacca gataaatata tatttacaat agctggagat ggtggagctt
    50101 atgcaatagg gcttggagaa actatgtcag ctgctactag aaatgataat gtaatatttc
    50161 ttgttatgaa caataatatg tttgcaatga caggagggca aatggctcca actactatag
    50221 aaggtcaata tacagcttct actccagcgg gtagaaatta cgcagatcat ggttcaacat
    50281 ttgatgttgt aaaagttatg ggaaatttag atatagcata tctagcaaga ggaacaataa
    50341 ctaaccctaa agaaataaat acaacaaaaa gatacatcaa aaaagctcta gaaaaacaaa
    50401 aagaaaagaa aggtttttgc ttaatagaag tgcttgtacc atgtccaaca aactggggat
    50461 taaagccagt agatgctatg aagagaattg aaaatgaact tgttccaaag tatccacttg
    50521 gagaatttat tgattaggga ggaaataata tgttgaatga aattatatgt gctggttttg
    50581 gaggacaggg agttcttaca gcaggaaaaa ttataatgta tgttgcatat aaggcagaaa
    50641 agaaaattac atggttccca tcttatggaa atgagatgag gggaggaagt gcaaattgca
    50701 atgttgttgt aagtgatgaa aaaattgcaa atccttgtgt aaaaaatcca gatatactat
    50761 tgggaatgaa cagcgtatca gtagataagt atgaaagttt aatgaaaaaa aatggttata
    50821 tgtttgtcaa tgaatcacta gtaagtaagg atagaaagta cagagaagac attaatgtta
    50881 taaaagtacc tgttactgaa atagcacaaa atttaggcaa tgaaaaagct gctaacattt
    50941 gcatgttagg tgctatggta aagagtttgg atttatttac aaaagaagaa tttgaaagtg
    51001 gtatgtgtga atactttgaa aatcaaggca aaggtaaatt caattctaaa aatgtagaag
    51061 catttgaagc tggatacgac tacgaatgaa tattttaaat atttaaaata attattgaaa
    51121 tacttatttt tatatgttag tatttaatta ataaaatata gaacgtcgtt ctggacaatg
    51181 ggacgaagtc tttaggtcgg aatacaagaa cggcgttctg tataatagaa ctagggggtg
    51241 ttaaattgaa agttttagta ataaatcctg gtggtacatc cacaaaaatt tctgttttcc
    51301 aagatgaaaa tgaaattttg aaaaaaaata taacccatac aagagaagat ttaaaaaatt
    51361 tttctaaagt atttgatgaa tatgattata gaaaacaact gatagtagat attttaagta
    51421 gtgagaatca tagtataaat tcttttgacg cagtagttgg tcgtggtgga cttatgaagg
    51481 ctataaaagg tggtacctat actgtgagtg aagaaatgat tgaggatatg agaaatgaaa
    51541 taaatgggga acatgcatca aatttaggtg cattattagc taaaactata gctgatgaaa
    51601 taggagtaca atcatttgta gtagacccag tttctgtaga tgaatttgat gatgtatcga
    51661 ggattacagg aatatcagac atagaaaaag caagttggtt acatgcatta aaccataaag
    51721 ctgtttcaag aaaaattgca caaaaactag ggggaaaata cgaggattat aactttattg
    51781 tagctcattt aggttcagga atatcgattg tagcacataa acaaggcaaa atgattgatg
    51841 gaagtggtgg taggtctgat ggtccatttt ctccagaaag gagtggtgga ttacttgcat
    51901 atcctcttat agaattgtgt tactccggaa aatatactca tgatgaaatg gtcgcaaaag
    51961 taagttcgat tgggggaatg tacgattatt taggaacaaa ggacatgata gaaattgaaa
    52021 acagaatcaa ggagggtgat gaaaaggcaa aacttataat ggatgcattt atttatcaag
    52081 tagcaaaaga aatttctatg tatggagctt cactttcagg gaatgtagat agagttatct
    52141 tgacaggagg gatatctcac tcagaaatgg tagtcaaagg agtagcagaa aaagtttcat
    52201 atctagctcc aatagagata gtaccaggag agatggaaat ggaggcactt gctctagggg
    52261 cacttagagt acttaatgga gaagaaatag caaaaactta ttaatattta ggagaaagag
    52321 tatgttaaaa gattttaata atttattaga agaggctaaa aagcttgaac ctgttattgt
    52381 aagtgttgct gttgcagaag acaaagaagt attaaaggct gtgaaatcag caacagattt
    52441 aaacattata aagccaattc ttgtaggaga caaggataaa atagaaagta tatcaagaga
    52501 aattggatta aaaggtcatg aaataatagg ttgtcaaaat cccgatgaat cagttaaaaa
    52561 agctgtagag atagttaaaa ataaggaagc taaagttctt atgaagggat tagtaaatac
    52621 aagtgtctat atgagagcta tattaaataa agaaacgggt cttagaacag gaagattatt
    52681 aagtttgctt gcagtatatg aattacctca atatcataaa cttctttatt gtactgatag
    52741 tggaataaat gtatcgccaa acttaaatca aaaaaaagat ataatgacaa atgcacttct
    52801 tactatgaaa agtataggca tggaaaatcc taaaactgcg atacttactg caaatgaaat
    52861 ggtagaccct aagatacaat ctacagtgga tgccaaccta cttgtagaga tggaaaaatc
    52921 gggagaaata ccaaagtgta tagtggaagg tccaatagca tttgatgtag catttgatgc
    52981 acatgcagca gaacataaag gtattaaaag taatattgct ggtgatgttg atttattagt
    53041 atttccaaat atagaagcag ggaatatatt aggaaagtca tggcttagat ttaataaagc
    53101 taagtgggca ggtatagttt taggagcaac aaatccgata attttaggtt cacgttctga
    53161 cacagctgaa ataaaaataa attcaatagc attagcgtgc ctagcatcac aataagaaat
    53221 tattattaag gaggaaaaag tattatgaaa tatgcaaaaa gaatggatat ggtaaaagct
    53281 tcagctataa gagattctca aaagaaaata gctcagaagg tagcaagtgg tggaactgtt
    53341 atatcttttg ctgcagggct tcctgaccca aatttatttc caatagaaga gataagtgaa
    53401 ataactcaaa aagtacttaa tgaaagggga aaatttgccc tagcatatgg acctactaaa
    53461 ggtgaagatg agttgttaga tctattagta aaaagaatga aagaagaaga aaatattgat
    53521 acaaaagcag aaaatattat aataactact gggtcacaac aagggatagc attaagtgct
    53581 atgattttat tagaaaaggg agatgtagta gttacagaaa atccaagtta cttaggtgca
    53641 ataaatgcat ttagacctta tgaatgtgat tttttaggag tagatacaga tgaagaagga
    53701 atggtaacag atgatttaga caaattatta tctgaaaatt ctaacataaa ggtgatatat
    53761 gtaataccaa catttcaaaa ccctacagga aaaacatggt ctttacaaag aagaaaagat
    53821 tttatggaag tcgtgaataa atatgatgtc atagtaatag aggataatcc ttatggagaa
    53881 ataagattta caccagaacc tctacctaca cttaaatctc ttgatactaa agataaagta
    53941 ttgtaccttg ggtcattttc aaaggtatta tgtccaggat ttagagttgc ttggatgtgt
    54001 ggtaatgtag acatcataga taaggcagaa cttttaaaac aaggtataga tttacagtca
    54061 aatcaatttt cgcaagtaca agtaatagaa tttttaaaga aatacaactt aaatgaacat
    54121 atagaaaaaa taagagcaga gtataagaaa agatgtctgc taatgttaga tgctatgaag
    54181 aaacatttcc cagaggaagc taaatataca gaaccagatg gaggaatgtt tatatgggtt
    54241 gaacttcctg aaagtataaa tgtagatgat ttattagata aagccattga tgctggagtt
    54301 gcttatgttt ctggagagtc cttctttgca aataatggtc caaaaaatac tatgagactt
    54361 aactatacaa caatgtcaga agaacaaatc gttaaaggtg ttgagatact tgcagaagtt
    54421 ataaaaaaag aactcaatta tgcttgtgaa taaatataaa aagtgagggt ttaaatatgg
    54481 gagaaaaaga aaacggtata aagtttggaa agttaatgtt tcaacttgtt attatagcaa
    54541 tatcttggga attagtttat attataccat ttatccaata tacattatat gatccaatcc
    54601 taaaagcatt agaatgtaca aacactcaac taggttttct acttacaatt tatgggttag
    54661 gtaatatatt tggagctcct ataggaggct ggttagcaga tagatttgat tatagaaaga
    54721 taattttagg ttctgtattt cttaatggag tagtatcatt tttatttgca tttaatatga
    54781 actatccatt tgcagttgct acttgggtag gttgtgcaat cacatcatta gttatgaact
    54841 atccttctat ggtaaaaata cttagagtta taggaaaaga taatcaaggt aaggtttatg
    54901 gatttaatga agctatggtt ggagtttctg gagtagtaat gggagcaata ttcttatata
    54961 tttatacatg ctttgcaact cctacattgg gtatgagatg ggtaatgata tctttaggaa
    55021 tattatcaat agcaatgtgt ccagtattat ggtttgtaat taaagatgta gatattaaag
    55081 aagaaagaga agaagcaggt gaaaaaatgt ctgctggtga ttttataaca gtactaaaat
    55141 caccaaatac ttggctagta ggaataagta tattctgtgt atattcattc acagttacaa
    55201 tgtcttattt tacaccatat ataacatctg tacttggagg ttctgtagca ttatctggtg
    55261 cattagcaat aataagacag catggtctaa aattatttgg agcaccattt ggaggatatt
    55321 gtgcagacaa attaaaatct cctacaaaag tacttttacc aatatatgta tttggtatag
    55381 cagtaatagt tttattccta gtattacctg catcaactcc aatgactata ttcatagctt
    55441 taacatttgt agtaggtata ttaggttaca tgggaaaagg tatatattat gcagtacaag
    55501 atgaagtaaa agttccagtt aaatattcag caactactat aggtatagct gcagctttag
    55561 gattttcacc agatgtattc caatttgcat taataggaca ctggatagat acttatggca
    55621 ataaaggtta cacatataca tttatattcc aaatagcaat tctagttatt ggaatactat
    55681 cttgtttata tatattaaag gttaaaaaga gaagagcaag taaattagaa gcttaaatat
    55741 tcaatttggt aattaagtat atataaatac aatacttatt ttgatgaatg tgattgtata
    55801 aataacataa aaaatatcaa tagattaagt tttataataa tgtttagaga ataaagtata
    55861 aaaatcaaaa tgttatcaag ttttctaggt tttaacataa agttacccct gaaggtgtaa
    55921 aattgagtgg caattattat ctattttctt acctacataa acctatttaa tttaaattct
    55981 aagagatttt caaaatagat ggtagttgta ctcaatttta aacctcataa taaatacgaa
    56041 aatttaaaat ctaactagat actatttaat tatatattaa taaatatata ttgtactatt
    56101 tatataataa taatttcagg cttttcatta tatgtattcc aatttatatt gtttgattat
    56161 tattcaaaca cacgtcgtta aaaattttat aaatttatgt ttagatatga ttaatactct
    56221 tgattttag
//
LOCUS       DAMJYR010000011        52957 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.011, whole genome shotgun sequence.
ACCESSION   DAMJYR010000011 DAMJYR010000000
VERSION     DAMJYR010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 52957)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 52957)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..52957
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.011"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            443..1981
                     /locus_tag="PC471_000665"
     CDS             443..1981
                     /locus_tag="PC471_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HDF2787806.1"
                     /translation="MSLLKVTNLSQCFMDKSLYEKANFDLFKGEHIGVVGQNGTGKST
                     LIKILLGEVVPDSGEIKWQPNINIGHLDQYAEINRDTTISLYLHTAFEELYKIEKEMN
                     LLYQKSAISENEQYLIKASDYQEQLIASNFYSIDNEINKIANGLGLDSIGMNRVVGEL
                     SGGQRAKVILAKLLLSNHDVLLLDEPTNFLDKEHVEWLSNYLNTFNGAFIVVSHDFDF
                     LEKISTGILDIEFGMIKKYHGKYSEFLKQKSHLREDYIRRYQAQQKKIEKEETFIRKN
                     KAGVNSKIARGRQKQLDKIERIAPPSFTGKPNIQFSEIEISAQNALTITNLEVGYYYS
                     LLPKLNFSVDGGQKIVITGFNGIGKSTLLKTLVKDIPRISGDFQFSEQVKIGYYEQDL
                     KWENPDKTPLQIVADKYPKLNTKEIRRHLARCGVKEEHVSRSVSTLSGGEQSKVKLCC
                     MMLSPCNFLILDEPTNHFDAETKDALQNALKQFRGSIILVSHEEKFYKGWIDKVFNIE
                     KQLV"
     gene            complement(2253..2966)
                     /locus_tag="PC471_000666"
     CDS             complement(2253..2966)
                     /locus_tag="PC471_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2787807.1"
                     /translation="MDTCFNKKILLVDDEKDIVDLIEEVLINDGFKNIIKAYNGLDAI
                     SLCKIACPDVVILDIMLPDIDGIEVCKKIREFSYCSILFLSSKNDDIDKILGLSSGGD
                     DYITKPFSPREIAFRVKAQLRRQQYQSIVPSDSEVIKIGDITIDIEGNRVYKDRNEIE
                     LTGREYHLLSYMAQNVNKIIGKERLYEQVWGVYSSICDNTIMVHIRHIREKIEDNPSN
                     PKILITVKGLGYKLVNRID"
     gene            complement(3414..4793)
                     /locus_tag="PC471_000667"
     CDS             complement(3414..4793)
                     /locus_tag="PC471_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HDF2787808.1"
                     /translation="MSDVYYSENHEQDNLYEQDNLLAQRFNLFTLLKFAFPTIFMMIF
                     MGLYTIVDTIFVSRFINTNALSAINIVCPIINLIVGIGTMLATGGSAIVARKMGNGDI
                     EGANKAFSLIVLSGVVIGVIITGIGIVFMDYIIIWLGASEVLIKYCRSYLFIILVFAS
                     ANILQVLFQCLFVTAGKPQFGLWLVLGAGCANIILDYVFIVLLQMGISGAALATGVGY
                     LIPTVSGMVFFSKNKGLLKFCIPRLDKRILFEACFNGSSEMVGQLSTAVTTFLFNAMM
                     MKLIGEDGVAAITIIIYSQFLLTSLYIGFSMGVAPIISYNYGSENNTQLNHVFKICIL
                     FISVASLLVFIFSISCSPIIVEIFSQRGSRVYEIARSGFYIFSFSFIFCGYNIFTSSM
                     FTALSNGKVSAILSFLRTFGFITIGLLILPNILNINGIWLAVPSAEILTLFLTIFCIC
                     KYKNRYKYM"
     gene            complement(5078..5872)
                     /locus_tag="PC471_000668"
     CDS             complement(5078..5872)
                     /locus_tag="PC471_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HDF2787809.1"
                     /translation="MMKKNLKIIFMLLKMKLSKMMVFRFDFFGAFFVDSSLFILQLLM
                     FNSIYSHVDSIGGWQQGEMLIFIGTFSLINAINMTIFFFGIYDIPRKIQEGELDYYIT
                     KPLDPLFRLTFENINIGSSPLILASILIVLYGISELNSEITLVMIIIYIFMVLLMTLL
                     FYDVCIILRTVSFFVISSSAIMRLEDNLLPMNMKIPGVIYEGVFKVLFHLILPYGIMS
                     TIPTQILAGTITINGLIYSILIVFLFTLFMWRFWKLGMRHYKSASS"
     gene            complement(5869..6732)
                     /locus_tag="PC471_000669"
     CDS             complement(5869..6732)
                     /locus_tag="PC471_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HDF2787810.1"
                     /translation="MSIMSNYNEASSNDSMFYKFKQSIRRYYEVAKINFKVQLIWRFD
                     VVVNMVFTIVKILFAYILWKAIFGENKIVSGFTFNSMLSYYIISSFLAGIDMSKSTSE
                     EICNRVKLGTFSKYMIIPINVQNYFIAKNFGMTCFYFVFNMISAFVWIFIFKIDFAFT
                     SNPVQILISILMIFLGFIFMMKLHFFIGILSFKFLEISFFKMIIENIVEFITGTMIPL
                     VLLPETIVTVMKVFPFYYTTYLPSMLLIGRNSNEAIQGLIILALWVLFFSVVNKVMYK
                     RMRTLFDGVGI"
     gene            complement(6710..7705)
                     /locus_tag="PC471_000670"
     CDS             complement(6710..7705)
                     /locus_tag="PC471_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDF2787811.1"
                     /translation="MSIIELNELTKAYRRFEKKAGFMGSVKSLFNRKYTEKTAILSFD
                     LKIEEGEFVGLIGQNGAGKTTLIKMLTGIIAPSSGEISVMGYYPNKLENNFKKQYAVV
                     MGQKSQLFFELTPADTFLLFKELYDISNEEYKKNLDYFIELFEVSDYLNVQVRTLSLG
                     ERMKMELIVALLHNPKILFLDEPTIGLDAIAQKQIRYFLKEVNETKGTTIILTSHYME
                     DIKSLCSRSIVVENSRKIYDGSTEKLFNHFQKYKKITVSFSNETNIVFPKDCKVVEQT
                     NHKISVIVPKERGNETIKWIFDKYDVKDVMIEEEDIGSIVERIYKKGSDEYYEQL"
     gene            8408..9364
                     /locus_tag="PC471_000671"
     CDS             8408..9364
                     /locus_tag="PC471_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HDF2787812.1"
                     /translation="MENNTIQKQLRDLSKKIWHIYILNPEEENLKFIISLLDDNLALI
                     GTGKHEFYETLEEFIIGLEKDQTESEQIDFEIIDEWYSCTKVTDEAFIVYGTLWVIEK
                     DYLEKDIYVEMDTRFTMVYRVTDDMIKLIHVHHSIPNFDQMKDEYYPKTITEKAQEAL
                     AMANEFKLKSEKDLMTGLYNRHFFEVHIKEMLKTVSHGYFYIFDLDNFKQVNDIFGHV
                     RGDEILIHFSNILTKVFVNDAIIGRLGGDEFAVFEYSNQSKENVENKAKKVLELCLES
                     NSCSIGISKLENNSDTFQSLYKQSDYALYYSKRNNKGNFSWF"
     gene            complement(9757..10647)
                     /locus_tag="PC471_000672"
     CDS             complement(9757..10647)
                     /locus_tag="PC471_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2787813.1"
                     /translation="MKKNILTTMITMIIILMMSMTVMAEPYQEDETSSLKKQSANERN
                     QTNIGTMDGRVQTNAISKGANYDPKYPLKGMLTQLGLNTTGKEGWDLLYGGNPNGVEL
                     RADGKYYYINKGMEGLGFYAAALSGGSYQRIGFGKLDPIEVIADGNWISYKQAQDTLT
                     VIRNFLNSFDWRNASEMERANRAAKLVTEAKYADSKYCNIVYGNLVDKRGVCGSFASS
                     FHLLTRLMGMDSLSILNPSLNHAWNYIQIDGKWYRFDGSEISAFGGSLDFDYRKLKDA
                     TREMTTYYDAKALSILGFNQ"
     gene            complement(10825..11523)
                     /locus_tag="PC471_000673"
     CDS             complement(10825..11523)
                     /locus_tag="PC471_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2787814.1"
                     /translation="MKISSQYRSQYSFRYESNINNTRINESMVKKNETVGKDTYLSNI
                     MKQKQELNDRIRDLKYRQEVYTKKINDAIKNLCKSEIRETTNNFSNIEIGIKNSIIEE
                     KNKSTMLDENSTYLNTNDEKESLITKESNEKIEEEILNDEKLEELEQKKDYKEDSNKK
                     EKVSEDLSLVGKTREELENMLKNFINLTQEEIMKLESRIEKLDKNAEEYKQNSKTNIF
                     DKTDEQKKHINVLI"
     gene            complement(12059..13126)
                     /gene="rsgA"
                     /locus_tag="PC471_000674"
     CDS             complement(12059..13126)
                     /gene="rsgA"
                     /locus_tag="PC471_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HDF2787815.1"
                     /translation="MNGMKQINLQDYGLNRNFITQSMEYHDLYLARIISQEKNFYQAV
                     CVNGVLFAEVSGKLRFEATIQSDFPTVGDFVMLDRCNNKNGNAIIHHVLHRKSLFVRK
                     AAGKVNEEQVVAANIDIVFLCMSLNKDFNIHRLERYLSLTWESGAIPVVVLTKSDLCD
                     NVHRKLQVVNSIALGVDVVVTSSIEPDGYKQISKYIKPGCTVALLGSSGVGKSSLINC
                     LMGGEFLNTKEIRIDDDKGRHATTRRELFLISGGGIVIDTPGMRELGMWDVSEGLDKS
                     FADIEAYFGKCHFRNCTHTSEPGCAIWEAIAHQELSPKRWESYRRLKTEAVFADNKSE
                     YLAKKEKKFKNISKINKKIRR"
     gene            complement(13123..13524)
                     /locus_tag="PC471_000675"
     CDS             complement(13123..13524)
                     /locus_tag="PC471_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNHCP domain-containing protein"
                     /protein_id="HDF2787816.1"
                     /translation="MNYKNRKRAYYQENSCMDNFICKNCGCLVVPEGAGTQHRNHCPH
                     CLHSLHVDIIPGDRAADCDANMEPIGVWVRKNGEWAIIHRCTRCGHLSSNRIAADDNS
                     VKLMSIALKPLAQPPFPLERIIEKLEKEETE"
     regulatory      14321..14417
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            14471..14647
                     /locus_tag="PC471_000676"
     CDS             14471..14647
                     /locus_tag="PC471_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2787817.1"
                     /translation="MFRDKMDRCTHMLTAYIGSSYDYCDFIDTQLDDFILEYGENVVE
                     SCLHQVMVLVSKYN"
     gene            15057..15695
                     /locus_tag="PC471_000677"
     CDS             15057..15695
                     /locus_tag="PC471_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2787818.1"
                     /translation="MSNQRKDFDITNDMFQESIPITTKIILEDMPSNDELNHVFSKGC
                     ERKMKKRKIVLITLLLIGVLLLGSILYNLFLGKTANISMLKESWNFDIPIPNKEIEVF
                     DTQDSINGDGQSYFIQGFSEKNFKKVFNLKGGIVVSKDNINEIEKYIDKFKRDSVNIN
                     KSNKNKIEEDFKKYKLEVKKDDKYIYKRNYENYVVLIIKKDEQKLYSLIWNQ"
     gene            complement(16168..17526)
                     /gene="rlmD"
                     /locus_tag="PC471_000678"
     CDS             complement(16168..17526)
                     /gene="rlmD"
                     /locus_tag="PC471_000678"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HDF2787819.1"
                     /translation="MLSKNEMYIVDIVDIGQGGVGVGKYNGITVFVEGGLIHDKVKVR
                     INKVKKNYAVGDIVEIIEKSPFRVKRVCSDNLRDCGGCQIQDLDYNKQLEMKTNEVKQ
                     VLSRIGKLEDVEVHDTIGMDNPYRYRNKAQFPIQKNNGIPIIGFYKKKTHDVISTEKC
                     VIQHEINDKIVKIIKTYIRAYKVSIYDEKTHTGLIRHLVTKIGFTTKEVMVVLVANGN
                     KLPYLKELASVLKENIPGFKTLVVNVNQKDTNAILGRENIVIYGDGKINDYIGDLVFE
                     ISPLSFFQVNPVQTKVLYDKALEYANLNDTDTVFDIYCGIGTISLFLAQKAKKVYGIE
                     IIDDAIKDAKINAKLNNIHNAEFYVGKAEEIVPKMYREGKTANVVVVDPPRKGCDEKV
                     LETIVSMNPERIVYVSCNPSTLARDLDYLNVHGFKCVEAQPVDMFPHSTHVETVAKII
                     KQ"
     gene            complement(17701..19461)
                     /gene="pyk"
                     /locus_tag="PC471_000679"
     CDS             complement(17701..19461)
                     /gene="pyk"
                     /locus_tag="PC471_000679"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HDF2787820.1"
                     /translation="MLNNVKKTKIVCTLGPASQSEEVLTQLMQNGLNVCRFNFSHGSH
                     EEHKERIDMAKKVREKLNKPVAILLDTKGPEIRTGNFEDPEVFLEEGQKFTITMKDIM
                     GTKEMCTVSYKGLAEDVKSGDSILIDDGLVGLRVKEINGEDIVCVVENSGIVKNHKGV
                     NVPGVKINLPAITPKDISDIEFGISQGIDYIAASFVRKASDVLAIREVLENNNATDIQ
                     IISKIENQEGVENLDEILKVSDGIMVARGDLGVEIPTEEMPIVQKMMIKKCNELAKPV
                     VTATQMLDSMIRNPRPTRAEVTDVANAIYDGTDAIMLSGETAAGKYPVEAVKMMATIA
                     KRTEETLDYDRLLKENGTNNVTVTDAISHATCTTAVDLNASAIITSTSSGYTARMVSK
                     FRPKSPIIATTNNEKTMNKLALTWGVYPVKSSVAGNTDEVIEKAIEAARQADYIDNGE
                     LVVITAGVPVGVSGTTNLIKVHVISEEIVQGIGVGTQTVEGKVRIIKNGNACVDFNEG
                     DILVSTATDAEMNNYIEKAGAVVTENGGMTSHTAIVGINLDIPVIVSAADITNLVKDG
                     EVVTVDASRGVVYRGSTRVL"
     gene            complement(19513..20472)
                     /gene="pfkA"
                     /locus_tag="PC471_000680"
     CDS             complement(19513..20472)
                     /gene="pfkA"
                     /locus_tag="PC471_000680"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HDF2787821.1"
                     /translation="MKTIGLLTSGGDAPGMNAAIRAVVRSAIYYGCKVYGINRGYKGL
                     LEEDLTEMNLSSVGDIIHRGGTILKSSRCEEFKTEEGRLKAVKILKKYKIDCLVVIGG
                     DGSFAGAQKLSDLGFPAIGIPGTIDNDLAYTDYTIGFDTAMNTIIDAIGKIRDTSSSH
                     ERVNIVEVMGRHCGDLALYAGLAGGAETIIVPEVEITVDEVALRLKTTQKRGKRHSII
                     VLAEGVGSASDLEKELKKESGADLRVTVLGHVQRGGSPTVSDRILASRLGVRAVELLL
                     DGKSARVVGIKENKIIDLEISEALAQKKVFDKEAYEMAKILSI"
     gene            complement(20681..24250)
                     /locus_tag="PC471_000681"
     CDS             complement(20681..24250)
                     /locus_tag="PC471_000681"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HDF2787822.1"
                     /translation="MNKDFAHLHVHTEYSLLDGFSRVKKLIKRAKELNMSSIAITDHG
                     CMFGVIDFYKTAIKEGIKPIIGCEVYTAARGLRDKDPNYDKYQGHLVLLAKNMEGYKN
                     LIKIVSTSYVEGFYYKPRVDMEELKKHNKGIIALSACLAGDVARALMNRNYEKAKQFA
                     LDYRDIFGEENFFLEIQDHNLPEQKEVNSGLVKLSKETGIPLVATNDVHYVNKEDSKI
                     HDVLMCIQMGKTLNDPNRMRFGSDEFYLKSREEMEELFPYALEAIDNTVKIADMCNVE
                     FDFNTIHLPKYDVPEGYTPETYLRELCFNGLKERYDNPSNEILERLNYELDVIEKMGY
                     VEYFLIVWDFINFSKENNIIVGPGRGSAAGSIVAYTLKITDIDPIKYSLLFERFLNPE
                     RISMPDIDIDFCYERREEVIDYVKRKYGDDHVAQIITFGTMGAKAAIRDVGRVLDIGY
                     NKVDKVAKEIPFALGMTIDKALDTNPNLKELYDQDPETKEIINISKQIEGMLRHASTH
                     AAGVVISKNPVDEYVPLYKHQDAITTQFTMTTLEELGLLKMDFLGLRTLTVIRDTLDL
                     IEKNRVIKNYTEKIDFSKMDYDDPKVYEMLSSGNTLGVFQLESAGMRSFMKQLKPDNF
                     EDIVAGISLFRPGPMDSIPAYIKNKSNPKDVTYLHEKLKPIMEVTYGCLVYQEQVMQV
                     VRDLGGYSYGRSDLVRRAMSKKKMDVMEEERQYFIHGKFDEDGNIEIAGCIRNGVEEE
                     IANKIFDDMIDFARYAFNKSHAAAYGVLAYETAYLKTYYPVEFMAALITSVMGNTDKV
                     VEYIRECKALEIDVLPPDINKSFSKFSVEGDNIRFGLAAVKNVGVNIINNIIAERESN
                     GLFVDLVDLVKRLDQKDTNKRVIESLIKCGAFDNISENRASLMAGYESLLESVSMDRK
                     KNVQGQISLFDAFGDSEEDSDFQQIYSLPKRQEYEERERLNLEKEVLGMYVSGHPLSQ
                     FEKELQEKTSMDNGKLNSLKEEEESYLQMNETDVIMGGMIVTKTIKTTKRNEIMAFIE
                     LEDLYGAIEIIVFPQLLQKYNVILNEDNIIYVKGTLSIKEGENAKLIAREIKDINDDT
                     DFADRNYNSGRTRNVENIKPESGKKLYLKIDSMSDTDTTNNIVEIAKQYPGNDSIYLY
                     PMDENINGKRKAYKMTGALTCICDEFINNLEELLPKDNIKVKA"
     gene            complement(24407..25354)
                     /gene="whiA"
                     /locus_tag="PC471_000682"
     CDS             complement(24407..25354)
                     /gene="whiA"
                     /locus_tag="PC471_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HDF2787823.1"
                     /translation="MSFSTETKNELARVISGNECCNIAELSALVKSGGSIQIVGYKKL
                     NLKITTELNSIARKVFKLLKKNFNINTTISVNKNQMLKRNNSYVLMVTSEMGSEMLLK
                     KLGILEDKEGFFTINKISENLIKHEGCKRAFIRGAFLGGGSISDPEKNYHMEFVTNNE
                     EFADSLKELINSLGFNSKIVARKNNHVVYIKESEQISDLLSIIGGHHALLSLQNTKIV
                     KEMRNNVNRIVNCETANLSKTVNAAVRQVENIKLIQETIGISSLPENLQEIAKIRIEY
                     EDMTLKELGEMLEPPIGKSGVNHRLRKIEEIATDLKKKS"
     gene            complement(25371..25796)
                     /locus_tag="PC471_000683"
     CDS             complement(25371..25796)
                     /locus_tag="PC471_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HDF2787824.1"
                     /translation="MREEVSAGGVVLFGNTILLLRKFNGDWVLPKGKVEEGENNQEAA
                     LREVSEETGVKADILKYLGEIHYTFKENWDENRAVHKTVFWYLMQAKNMDTIPQKEEG
                     FIDAKFIHLDRVVDLARYDDEKEIIKVALQEIKKRLKKN"
     gene            complement(25865..26971)
                     /locus_tag="PC471_000684"
     CDS             complement(25865..26971)
                     /locus_tag="PC471_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HDF2787825.1"
                     /translation="MAKLLIIVGILGWIALIVSLFIYKKAKNEKVKLFQRSIVENREP
                     NVVVIGGGTGQSVFLRGLKHTTQNITAIVTVADDGGGSGVLREDLGMLPPGDIRNCLL
                     ALANIEPTMNEVMQYRFTEGLLKGQSFGNLFLAAMNGLYGNFEKAVYKLSEIFAITGR
                     VLPVTLEDVNLVAKLENGNIINGESSIPEESKIQKSSIDKIFLNPKDVKPLKDVIASI
                     YDADIIIMGPGSLYTSIIPNLLVEGIVDAIKSSVAPKVYIANIMTQPGETEGYNVLEH
                     VNAIVKHTDENLIDYVIANNEILPEEMLDLYKQDGAEQVLLDKKQKDKLKEMGIKTVE
                     KNLIEIKNNYIRHDAKYISNIVIELALNHNYNKS"
     gene            complement(26983..27840)
                     /gene="rapZ"
                     /locus_tag="PC471_000685"
     CDS             complement(26983..27840)
                     /gene="rapZ"
                     /locus_tag="PC471_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HDF2787826.1"
                     /translation="MKFVIVTGLSGSGKSETMRALEDMGFYCVDNLPPALITKFAELC
                     YQPNSSIDKVALGIDIRGRKFFEALHESLNYLEKENYEYEMVYLDCNDDVLLKRYKMT
                     RRNHPLAKDMQIPEGIKMERKIMEPLKELSTCIIDTTNMKPKDLKEEIKKIYSSGEDN
                     PNLTISVVSFGFKHGILADADLVFDVRFLPNPYYVEELRAKTGDDKEVRDYVMNSKIS
                     EEFYVKLLDMIHFLVPQYIEEGKQHLVIGVGCTGGRHRSVTITNLIAEDLSNKGYRVV
                     KKHRDSMLR"
     gene            complement(27871..28584)
                     /locus_tag="PC471_000686"
     CDS             complement(27871..28584)
                     /locus_tag="PC471_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HDF2787827.1"
                     /translation="MKILADYHTHTLYSHGKGTIEDNVKVAISKGIKTIGISDHSYKH
                     IAYGVKKKDILKMREEVDYLNDKYTDINILLGMECNILDDKGNIDMDDKFIRDFDYIM
                     AGYHFGSTPTSLRSLLNHCNNYIFKTEKAKEYNTKAVINAISNNDIFIITHPGDKGDV
                     YIEEVAKTAKKTNTRLEINSSHSFLNVNQLNKIKGLENKFIIGSDAHCPERVGDFELA
                     MKTISDSNLDISLVENIII"
     gene            complement(28588..29502)
                     /gene="murB"
                     /locus_tag="PC471_000687"
     CDS             complement(28588..29502)
                     /gene="murB"
                     /locus_tag="PC471_000687"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HDF2787828.1"
                     /translation="MNNQDIYINLLNILSKEDIKVDEPMKKHISFRVGGPADILVRPR
                     TEEQLKNVLKLVKKESIPYLIIGNGSNILIKDGGIRGVVIELADNFNSYEINDTRMTA
                     QSGALLSVLGKALQKQELKGFEFASGIPGTLGGALAMNAGAYGGEMKDIVKSVRLMDM
                     EGNIFELSNEQMEFGYRKSIISKNGYIALSAELELQEGNYDEIKSLMDDLATRRITKQ
                     PLNFASAGSTFKRPTGYFAGKLIEETGLRGLTLRGAQVSEKHCGFVVNQGEASAKDIL
                     DLIYVIKSAVYAKFGVMLEEEVKILGED"
     gene            complement(29775..30545)
                     /locus_tag="PC471_000688"
     CDS             complement(29775..30545)
                     /locus_tag="PC471_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HDF2787829.1"
                     /translation="MSKGFLTPGETAQATISAGIKKANISTQNAILLGLFGGAFIGFG
                     ALGSMIVSQTFGKIDPGVASFLSAMVFPVGLMLVVVAGAELFTGNTLMAIALMDKKIT
                     LGKMLRNWGLVYFANLVGSILLVALVFYSSTLTGDAATKAIAVAEAKVNSTIASMFLK
                     AILCNILVVLAVWMATASQDIMSKLASCWTVIMLFVLCGFQHSVANMFFIPMGMALGA
                     NITMVQFITNLVFVTLGNIVGGSIVVGGIYYLCYVKNS"
     gene            30863..32344
                     /gene="cls"
                     /locus_tag="PC471_000689"
     CDS             30863..32344
                     /gene="cls"
                     /locus_tag="PC471_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HDF2787830.1"
                     /translation="MFDISFFEMSIYEIAVTTIYIINFMVILNLIFREKRNINTTLTW
                     LLILVLIPALGFILYIAFGRNISKNNMFRLKEKDDKIIKSNILDTQVKLQSTSEIDSD
                     IHQHKDMIYALANSNNAHYTNNNDVWIYAESSQFFNSLLEELKKAKKYINIQFYIFKD
                     DKIGTEIIDILIDKAKEGVEVRLLFDAVGGRTLKNSTLSRLKESGVKVGSFFPSFLKI
                     VNFNLNYRNHRKIVVIDGKVGFVGGYNVGDEYLGRNPKFGLWRDTHTKLTGDCVIDLN
                     MRFILDWRYTTKEDLDLEKYFIEEDTNSNHPSENIGIQIVSSGPDISELDEIKYGYLK
                     MIQKARKYIYIQSPYLILDSTFIDTLKIACLSGVDVRVMIPSKPDHPFVYWASYSYAG
                     ELLKFGAKIYTYGQNAFLHAKTIVIDDSICSIGTANMDIRSFELNFEVNAFMYSSKKA
                     LEQRIIFENDILNSKEITLDIYNSRSTYIKIKESISRLLSAVL"
     gene            32662..33159
                     /locus_tag="PC471_000690"
     CDS             32662..33159
                     /locus_tag="PC471_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HDF2787831.1"
                     /translation="MCDFISHNKQLFDELDIFIDSLATKEGALIQVLHEAQGIFGYLP
                     KEVQLHVARKLGVAPAKVYGVVTFYSYFTTEPVGKYKISVCLGTVCFVKGADKILSAF
                     EKQLGIKVGETTSDFKFSLEGLRCLGACGLAPVVTVNGKVYGKVKPDQVSEILDTYRE
                     LELNC"
     gene            33176..35062
                     /locus_tag="PC471_000691"
     CDS             33176..35062
                     /locus_tag="PC471_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HDF2787832.1"
                     /translation="MRKVNSFDELKVLADELKPNLSLRKNYSKENVLRRELLVCCDTG
                     CTSSNSLEIVSELENEIKKSGIQDKVSVRLTGCFGFCAQGPIVKVYPDNVFYVKVEPS
                     DAEKIVQSHLIRNTVVEELLYEEESLSKKVEEQEEMSFYKKQLRIALKNCGLIDLDSI
                     EEYIANDGYLALGKCLTSLTPQEVIAEVKTSGLRGRGGAGFPTGSKWEAASKNPAGPT
                     DKKFVVCNADEGDPGAFMDRSVLEGDPHSVLEAMAICGYAIGSDTGYIYIRAEYPKSI
                     ERLKVAIAQAEKYGLLGENILGTGFNFKLELKYGAGAFVCGEGTALMHSIEGRRGEPR
                     MKTYSSSKSGLWKSPTCLNNVETFANIPAIILKGGDWFANLGTEDSSGTKVFALGGKV
                     ENVGLVEVPMGTTLRDIVFEIGGGIPEGKKFKAVQTGGPSGGCIPTEHLDTPIDFGSL
                     SSIGSMMGSGGMLVLDESDCMVDIAKFFLEFTVEESCGKCTPCRIGTTRLLEILTKIT
                     EGNGTLQDLDDLENLAETIQTASLCGLGKAAPNPVLSTLQYFKDEYIAHVVDKKCPAG
                     KCQNLLSYFITDDCKGCTKCSRVCPAGCITGSVKEQHTIDTSKCLKCGACIDNCTFNA
                     IIKK"
     gene            35085..36866
                     /locus_tag="PC471_000692"
     CDS             35085..36866
                     /locus_tag="PC471_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron hydrogenase small subunit"
                     /protein_id="HDF2787833.1"
                     /translation="MSLVNLTINGKHVSAPSGTSILDAAKLINIKIHNLCHLHMNEID
                     KLDTCASCRVCMVETERGLVPACGTVIKEGMKVQTNSAKALNARRTIVELLLSDHPQD
                     CFVCEKNGDCELQTIAADLGVRKIRYQGSKSFAGKDTSTKSLVKDHSKCILCRRCETV
                     CNDIQTVGALSGVNRGFNTLVSTFFNSDMVETECTFCGQCISVCPTGALTEVDNVPKL
                     WDVLNKKEKTIVVQVAPAVRVAIGEEFGLEPGSISTGKMVAALKALGFEHVFDTNFGA
                     DFTIMEEATEFIERIQKGENLPILTSCCPAWVNFLEHNYPDKLNLASSCKSPQGMFGS
                     IAKNYYAPKILGINPEELYVVSVMPCVAKKYEASREELSESGILDVDLSITTRELAKM
                     IKEAAIDLPNLQDQDFDNPLGKSTGAASIFGASGGVLEAALRTSYEKITNKTLDNVNF
                     TNVRGLKGIREASIDVDGTTVNVCIVNTLKNARKIMDKVRSGECKYHIIEVMACPGGC
                     VGGAGQPYHHGNTEIVDRRANALYEIDRNKAIRKSHENPDLQAIYKDFFGEPNSDVAH
                     KYLHTHYFDKSCVYGECPQECACEEAK"
     gene            complement(37006..38802)
                     /locus_tag="PC471_000693"
     CDS             complement(37006..38802)
                     /locus_tag="PC471_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate oxidoreductase"
                     /protein_id="HDF2787834.1"
                     /translation="MGIEYFSKLLESSTEKELLLSKKSNNISTARLISFLIVIAGLAI
                     GFYNKNIVGIFVGVLSIIIFIALLVIHSKVKEEETYFKSKSEVLNKYIKRFGDGWKEF
                     KIDGKEYLKDENSQAKDLDLFGRASLYQYICVANTSYGKNFLAKYLWNENPDENIILE
                     RQRAIKELLSKDDFSIHIQTLSNIIGKEQKNNSDSSIESFIEYGENKNVYIPKWMHIF
                     TWGLPTATILSFIFCMLGFLPVLPVFLLFIIQLGFSGFGYPRLMQTLAPLFSFSRSIQ
                     VYEKMFEVLEKETFESAYLKELQDKLSKGSGVSRGVKQLNSIGNAVNLRYNQILYIIA
                     CGVLMWNYHCAEALERWKGIYGNQIRDWFESIGEFEALISLTVVSHVKENTCFPVIKY
                     EDTPRLKVEEVYHPLIAEKSVIANSIQLNSQTCIITGSNMSGKTTFLRSIGVNLVLAY
                     AGAPVCAKSFDATCMAIFTSMRIQDDVSQGISTFYAEILRIKSMIQYSLKELPMLVLV
                     DEIFKGTNSADRIIGASEAVKKLSKPWVISMVTTHDFELCDLSGSGDVEVVNYHFSEY
                     YVDDKIHFDYKIKDGRCKTTNAKQLMRMAGIL"
     gene            complement(39014..39943)
                     /locus_tag="PC471_000694"
     CDS             complement(39014..39943)
                     /locus_tag="PC471_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HDF2787835.1"
                     /translation="METSNKVSIREIIKELKLKVVYMPDDVDYYVYSQDINRPGLQFA
                     GYFDYFAYERIQIVGKTEYNFFGLMDSHIREEVLDRFFSYEIPALIISRDLEIKPDVI
                     EKAKKYKRILLSTNRNTTRLINRLSNYLDNKLAPHTTIHGVLVDIYGIGVLIKGESSI
                     GKSETALELIQRGNRLVADDAVEIRKLDESMLVGQSPELIRHFLEIRGIGIIDVRSLY
                     GVGAIKNSKMIDLVVHLEAWDETKYYDRLGLDKEYEEILGVNVEKLVVPVKPGRNTAM
                     ILEVAAMNFRQRGMGYDAAQEFTKKLSKLIDTK"
     gene            complement(39943..41760)
                     /gene="uvrC"
                     /locus_tag="PC471_000695"
     CDS             complement(39943..41760)
                     /gene="uvrC"
                     /locus_tag="PC471_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HDF2787836.1"
                     /translation="MFDIQEHLKKLPSEPGVYLMKDKYDHIIYVGKAISLKNRVRQYF
                     QSSKNHTSKVKSMVKNIYKFEYIITDSELEALILECNLIKRYRPKYNVVLRDDKTYPY
                     IKVTTNEDYPRILKVRRVLKDKAKYFGPYTNITAVNDTLELISSTYPIRSCKIDIDKA
                     IKNKTRPCLNLHINKCLGPCTGNVSKEEYGKMIEEIIMCLSGKEEKLMELLKEKMNES
                     SMNFRFEEAAVYRDKIKSLEEMIQKQKIDATVSDLNQDVVAMARAHNEACVQVFFIRN
                     GKIVGREHFILEGVMDSPRASILSSFVKQFYNEQEYIPKELIIEDEIEDSSILEEWLS
                     SKKGQKVTIRVPQKGEKKSLVEMVRKNAVEYLEKFSDMNKRKYEKSIGALEELKQILN
                     LEKLPIRIEAYDISNIQGVDSIGSMVVYTNAKKDKKEYRRYKIKTVIGPNDYDSMAEI
                     VDRRLKHGNLPDLILLDGGKGQVSAVKKVLELNDVDIPLWGMYKDDKHRTKGLICKEK
                     EIELDKTTNLYRFIASIQEEVHNYAITYHRSLRNKALTKSILDDIQGIGEKRKKSLLN
                     HFKDVDAIKKATMEELLEVDGMNKSIADNVYNFFRKEEN"
     gene            complement(42019..44844)
                     /gene="uvrA"
                     /locus_tag="PC471_000696"
     CDS             complement(42019..44844)
                     /gene="uvrA"
                     /locus_tag="PC471_000696"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HDF2787837.1"
                     /translation="MEDKIIIRGAKEHNLKNIDLELPRDKFIVFTGLSGSGKSSLAFD
                     TIYAEGQRRYVESLSAYARQFLGQMEKPNVEYIEGLSPAISIDQKTTSKNPRSTVGTV
                     TEIYDYLRLLFARVGDVYCPTCGEKISQMTIQEIVDKMLDFPDRTKLQILSPIVRGQK
                     GTHKKVFDNIKKEGFVRVRVNGENYEVSDDIILNKNQKHNIEVVVDRIVIKDGIESRL
                     ADSIETAVKLSDGLVIAQIIDGEEILFSTKFACPEHGIGIEELSPRMFSFNAPFGACD
                     VCNGLGESKEVDPELVVPNKDLSIKQGAVAAWGSTGVNDDTYYSKMIKSLAEHFNVSL
                     TTPFKDLPDDFVEELLYGKDNIIVEFTYESKFGGTRNYKSYFEGVIVNLERRYRETNS
                     DYMRDKIEEYMAERPCPKCKGMRLKKEVLSVLVDGKNIMEVTNLSVNELIDFMENINL
                     TEKQRFIAHEIIKEIKGRAMFLRDVGLDYLNLSRKAGTLSGGEAQRIRLATQIGSALV
                     GVLYVLDEPSIGLHQRDNDRLITTLRHLTDLGNTLIVVEHDEDTMREADYVIDIGPGA
                     GIHGGEIVAQGTLDEIIENENSITGQYLSGKKQILLPETTREGNGSFIEIVKASENNL
                     KNIDVKFPLGKFTCITGVSGSGKSTLINDILYKGVASKVNRLKQRAGKHKEIKGIESI
                     DKVINIDQSPIGRTPRSNPATYTGVFDFIRDIFASTNEAKARGYKKGRFSFNVKGGRC
                     EACKGDGIIKIEMHFLPDVYVPCEICKGERYNRETLQVKYKDKTISDILDMNVEEAVE
                     FFENIPNIKRKLETLMDVGLSYIKLGQPSTQLSGGEAQRIKLAAELSKRPTGKTLYIL
                     DEPTTGLHMADVDKLIQVLQRLADTGNTIIVIEHNLDVIKTCDYVIDLGPEGGDKGGT
                     IVATGTPKEVSKVDGSYTGQFLKKYFS"
     gene            complement(44863..46833)
                     /gene="uvrB"
                     /locus_tag="PC471_000697"
     CDS             complement(44863..46833)
                     /gene="uvrB"
                     /locus_tag="PC471_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HDF2787838.1"
                     /translation="MDFKIKSDFKPTGDQPEAIKSIVDSINRNEKFSTLLGVTGSGKT
                     FTMANIIQQVKKPTLIMAHNKTLAAQLYSEFKEFFPDNAVEYFVSYYDYYQPEAYVAH
                     SDTYIEKDASINDEIDKLRHSATASILERRDTIIISSVSCIYGLGDPKDYKELMLSIR
                     PGMQRDRDDVIKRLIEIQYERNDINFTRGTFRVRGDILEIFPASNDEKAIRIEFFGDE
                     VDRITEIDYVTGKIVGTRNHVVIFPASHYVTTPERIEKAIVEIENELQEQIKFFKEND
                     RLLEAQRIEQRTKYDIEMLKEIGFCQGIENYSRHITGRSEGERPYTLMDFFPDDYLII
                     VDEAHVTIPQVRGMYAGDRSRKTSLIENGFRLPSALDNRPLNFQEFEGNINQMLFVSA
                     TPGPYEIQHSETIAEQIIRPTGLLDPIVEVRPINNQIDDLVGEITKTIEKNERVLITT
                     LTKKMSEDLTNYLKEIGIKVKYLHSDIVTLERTEIIRDLRLGKFDVLVGINLLREGLD
                     IPEVSLIAILDADKEGFLRSETALIQTIGRAARNENGRVIMYADRITDSMQNAIDETK
                     RRRDIQNLYNEEHNIIPKTIQKNIRDSIEATKVAEEEVVYGISDTDDKDEIRANIDKL
                     KSEMMEAAQNLQFERAAELRDKVKQLEEKLEK"
     gene            complement(47020..48282)
                     /locus_tag="PC471_000698"
     CDS             complement(47020..48282)
                     /locus_tag="PC471_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HDF2787839.1"
                     /translation="MTKVIVLSVDSLFEKDLEFVKNLPNFKSILENCSIVKNINCVYP
                     TLTYPCHTSMVTGVYPKKHGIYHNEKFDPNKENKDWYWYSKDIKAKTIIDVAKENNLT
                     TSSVLWPVMGANPNIDYNIAEIWAPSREDDPRETFERSSSKVIMDGIYNRHCHYIDWK
                     LEPNMDLFGVNCSVDIIKEYKPDLMLIHLATLDHTRHNYGLFNDEVDKALKMNDKWLG
                     DIIQATKDAGTYKDTNFIILGDHGHLRVDKVINPNVLLKSNGFIKVENGEVKDFDAYV
                     NSSGISAQIIVNNLDRLTELRELLEEFKEELFIENIFTKEEASNLGLEGDFEMVIEGL
                     EGISFGNDFEGSIIKDSDIKDYKFAVATHGHLPTKGNRPPFIAFGPNIKGGVVIEEGD
                     LRAHAATILKMFNLKLDGVEKEAFDFIM"
     gene            complement(48345..49649)
                     /locus_tag="PC471_000699"
     CDS             complement(48345..49649)
                     /locus_tag="PC471_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDF2787840.1"
                     /translation="MKLKRKLATGLAITLLLCSLVGCSNSNDANASKGEKTDSNITKI
                     DFWAPLGGTNGETAQKMVDQFNSEHKDVQVNMLKQKDYYENATKLQAALTSKDQPDVT
                     LLEITQTGTFASAGALVDMSKYFDKTYQERFFSGLLTNSYYEDKFVGMPFNRSTPILY
                     INKDMATKAGLDPSGPKSWEELKDYASKMTNKSEDTYGFETPIDIWFYEAMVMQSGGE
                     ITNGKKVAFNNEAGQAPVKFWQDMMKAGIMKMPPGEDYNAWDVAKQDFVNGKVGMIFT
                     STADLAGLMQQTEGKFEISTAFLPKNQKYATPTGGANLVMLEGGTDKEKEASAEFIEW
                     MTQTDKIVQFSSSTGYLPTTEDATNSEKLQTLYKEKPQYKVATDQLQYAVALPMLNGY
                     KEATDKLMDEIKKGLTDLNANPKDVVSKGTSAMQAVIDKTNK"
     gene            complement(49680..50540)
                     /locus_tag="PC471_000700"
     CDS             complement(49680..50540)
                     /locus_tag="PC471_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDF2787841.1"
                     /translation="MDIIQPIKKDKVDIKKIASKSIEILILLLLSAIFIFPFIWMLST
                     SFKNPQEMMQLPPTIIPNDIVLENYSTAWHSGPFFRYLLNSVFVTGSVLIVQFLVMVP
                     AAYAFSKIKFKGEKMLFGLLLVGLMIPPQVTFLPVYMMMSKFGFINTYVPLIIPFMTS
                     SFGIFLLRQNFMQISDEIIEAAKLDNASDLKIMFRIMVPMAKPAVITFILFNFIYHWN
                     NYFWPLVMTNTDAIRTLPIGVALLKSSEGITAWNVIMAGNIILILPIILVYIFANKKV
                     KEAFMYSGIK"
     gene            complement(50584..51453)
                     /locus_tag="PC471_000701"
     CDS             complement(50584..51453)
                     /locus_tag="PC471_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDF2787842.1"
                     /translation="MKKQFNKAYLYITPAIIGTLLFIVYPIIKTISLSFKSGSLLSST
                     FENVGLNNYTELLKNPEFIQTITNTAIYTFFMVTISISLAIVLAVWLNKNSIIHNLTQ
                     SIIFTPHVISLVSVAVLWLWIMEPQYGFLNWILSTLHLPTSKWLSSESSALMSLILIG
                     IWKTLGYNILIVLSGLKSIPNYIYEASKLDNANKITNFFKVTLPLLSPTIFFLMITTT
                     TASFQVFDEISIMTQGGPVGSTNMLSYYIYESGFIYYDIGQASAASMFLLAIVMIATY
                     ANFRLLSKKVHYQ"
     gene            complement(51437..52507)
                     /locus_tag="PC471_000702"
     CDS             complement(51437..52507)
                     /locus_tag="PC471_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2787843.1"
                     /translation="MADIRLENVTKSYNKKNTVIENLNLTIEDGSFTVLVGPSGCGKS
                     TTLRMIAGLEDITSGRLKIEENDVTKTDASKRDIAMVFQNYALYPHMTVRENIEFGLK
                     NKKVEKEKREKLITEVIDVVGLREYLNVKPGNLSGGQRQRVALARAMVKNPKVFLMDE
                     PLSNLDAKLRNQMRTELIQLHQKLKSTFVYVTHDQVEAMSMADKIVIMNKGKIMQIGS
                     PKKIYQNPKNLFVAQFIGSPCMNILDIKNDIKFGFRPEKATLESINIDGKNDDVFSLF
                     GKVVTKEVLGNETIYCLSVLNREIRVKTENDIFDIGRTLRISVLEKDLYYFDKNGERI
                     EDVKVCKSMYSKIKGDIYEKAI"
BASE COUNT        17312 a         9022 c         6428 g        20195 t
ORIGIN      
        1 taacttaagc tataattaat aaaacatatt ttaaataaat aagataatcc acaaagtaca
       61 aaagtacaat ttaaatacag ataaactaga attaatacta agattaggat gtgaataatt
      121 atgatttata tagaattagt taaacttgtt agaaaactta actctacaca aatgtatacg
      181 atgaagcgtt gtgtagaggt taatctgaaa gcttaaagct ttctatcttc atcggcaaat
      241 ataggtttag tatagttttt tggctatgct tatttaaaat tacctatttg ccgatagata
      301 atatagattt tctggtcata gacaacaatg cttgtctatg accttttgtt tttccaaaag
      361 cagaggtcgg cattttacaa tgccattcct ctgctttttt tatttccata aattttttaa
      421 taaaagaaaa ggagtttgaa ttatgagttt attaaaagta actaatttat cacaatgttt
      481 tatggataaa tcactgtatg agaaagctaa ctttgattta ttcaaaggtg aacatatagg
      541 tgtagttggc caaaatggta caggtaaaag tacattaatt aaaattttat tgggagaagt
      601 tgttccagat tcaggagaaa tcaagtggca acctaatatc aatataggcc atttagacca
      661 atatgcagaa atcaatagag atacaacaat atcactctat ttacacactg catttgaaga
      721 actttataaa atagaaaagg aaatgaattt actctatcaa aaaagtgcta tatctgaaaa
      781 tgagcaatat ctaatcaaag catcagatta tcaggaacaa ctaatagcaa gtaatttcta
      841 ttctatagat aatgaaataa ataaaatagc aaatggtctt ggattagatt caataggaat
      901 gaatcgtgtt gttggagagc ttagtggtgg tcaaagggca aaggtaattt tagcaaaatt
      961 actactttct aaccatgatg ttctattatt agatgaacca acaaactttt tagataaaga
     1021 acatgttgaa tggctctcaa attatttaaa tacatttaat ggggcattta ttgtagtttc
     1081 ccacgatttt gactttttag aaaaaatttc tacaggtatt cttgatatag aatttggcat
     1141 gattaagaaa tatcatggaa aatattcaga gttccttaaa caaaaatcac atttaagaga
     1201 agattatatt agaaggtatc aagcacaaca aaagaaaata gaaaaagaag aaacatttat
     1261 acgtaaaaat aaagctggag taaattcaaa aattgcaaga ggaagacaaa aacaattgga
     1321 taaaatagaa agaatagctc ctcctagttt tacaggaaaa ccaaatattc aattttcaga
     1381 aattgaaata tcggctcaaa atgctcttac tataacaaat cttgaagttg gatactacta
     1441 ttctttactt ccaaaattaa atttctctgt tgatggaggt caaaaaatag ttataactgg
     1501 ttttaatgga atcggtaaat ctaccttgtt aaagacatta gtcaaagata ttcctcgtat
     1561 atctggagat ttccagtttt cagaacaagt taaaattggt tattatgagc aagatttaaa
     1621 atgggaaaat cctgataaaa ccccacttca aatagtagca gataaatatc caaaattaaa
     1681 tacaaaagag attagacgtc atctagccag atgtggtgtg aaggaagagc atgtttcaag
     1741 gagtgtatct actttaagtg gtggtgaaca atctaaggtt aaattatgtt gtatgatgtt
     1801 atctccttgt aattttctta tcctggatga accaaccaat cactttgatg cagagacaaa
     1861 ggatgcttta caaaatgctt tgaaacagtt tagaggaagt ataatcctag tttcccatga
     1921 agaaaaattt tataaaggtt ggattgataa agtatttaat atagaaaaac aattagtata
     1981 agtttataca caatatacaa tattatttaa ttagtatttc aatgcaaatt ttaatgttct
     2041 agttaatgtt tcactgcaaa taaactaaaa aaattttatt agaagataaa gtacattacc
     2101 aggatataaa cttaatgagg ctatctcaaa atagagatag acctcttctt cattgttgag
     2161 ataacgaaaa tatatttgat tttacccata aaagaatgcc tcattaacta aaaattcaac
     2221 ttgagatatc ttcattaata ttaaaatcta ttctaatcga ttctattcac aagtttatat
     2281 cctagtcctt taactgttat aagtatttta ggatttgagg ggttatcttc aattttttct
     2341 cttatatgtc gtatatgcac cataattgta ttgtcacaaa tactactata tactccccat
     2401 acttgttcat agagtctttc ttttcctata attttattta cattttgagc catgtagctt
     2461 aaaagatgat actcccttcc agttaactca atctcatttc tatctttata tacacggttt
     2521 ccttctatat caatagtaat atctcctatt ttaataactt cactatctga tggtacaatg
     2581 ctttgatact gttgtctacg taattgcgct ttaactcgaa aagcaatttc tcttggactg
     2641 aatggctttg ttatatagtc atctccccca ctactaagac ctagtatttt gtcaatatca
     2701 tcattttttg atgataaaaa aagaatagaa cagtatgaaa actctcgaat ttttttacac
     2761 acttctatac catctatatc tggtaacatt atatcaagaa tgacaacatc tggacatgct
     2821 atcttacaca gcgaaatagc atctagacca ttataagctt taataatgtt tttaaaacca
     2881 tcatttatga gtacttcttc aattaaatct actatatcct tttcatcatc aacaagtaat
     2941 atttttttat taaaacatgt atccatattg aaatctctcc ataaaaattt attatattct
     3001 aatcttgcag tatcactatc ttgtttttta tattcactac tcttgctttt acattactaa
     3061 tagtaacatc tctaatagac aaattcaatc actatgaaaa ttaagataag tttctttaaa
     3121 ataaagtgta tgatacatta aaacctatag aaaattaatt tcatccaaag tttataatat
     3181 tagactaata cacttacaga tacagattaa tacaatagta aaataaaagg agttgtctta
     3241 aaattgactt tttctaatca aactttaagt tcaactccct tttaaacttt tatatgttat
     3301 ttttattaat tctttatatc aattataact ttaattttca atagtatata tctcccacta
     3361 attgaattac ttattaaaac ataaaatatc tacctaaact atttatttaa tatctacata
     3421 tatttatatc tattcttata cttacatata caaaaaatgg ttaaaaataa agttaaaatc
     3481 tcagcacttg gtactgctaa ccatatccca tttatattta atatatttgg caatatcaat
     3541 aaacctatag ttataaatcc aaatgtacgt agaaaagata atattgcaga cacctttcca
     3601 tttgataatg ctgtaaacat agacgaagta aaaatattat acccacaaaa aataaaacta
     3661 aaagaaaata tataaaatcc agaccttgca atttcataaa ctcggcttcc tctttgagaa
     3721 aaaatctcta caatgattgg tgaacatgat atagaaaaga taaataccaa taaagatgct
     3781 actgaaataa ataatataca aatcttaaaa acatgattta actgcgtatt attttcactt
     3841 ccatagttat aacttattat tggtgcaaca cccattgaga aacctatata aagcgaagtt
     3901 aaaagaaatt gagaataaat aattattgta atagctgcaa ccccatcttc accaattaat
     3961 ttcatcatca tagcattaaa taaaaaagta gtaactgctg ttgatagttg acccaccatt
     4021 tcagatgaac cattaaaaca agcttcaaat aatattcttt tatctagtct aggtatacaa
     4081 aatttaagta accctttatt ttttgaaaag aaaaccatac ctgaaacggt aggaattagg
     4141 tatcctactc cagttgcaag tgctgctcca ctaataccca tttgtaacag aacaatgaac
     4201 acatagtcaa gaattatatt agcacaccca gcacctaaca ctagccataa tccaaattga
     4261 ggctttccag ctgttacaaa taaacattga aataatactt gtagtatatt tgcagaagca
     4321 aaaaccaaga taataaaaag atatgaacga caatatttaa ttaaaacttc acttgctcct
     4381 aaccatataa tgatataatc cataaataca atacctatac cagtaattat taccccaatt
     4441 acaactcctg ataatacgat taaagaaaaa gctttatttg ctccctcaat atcaccattt
     4501 cccatcttac gtgctacaat agcacttcct ccagtggcaa gcatagttcc aattccaact
     4561 ataagattaa taattggaca tacaatatta attgctgata gagcatttgt attaataaat
     4621 cttgatacaa atatagtatc tacgattgta tacaacccca taaaaatcat cataaaaata
     4681 gtaggaaatg caaacttcaa tagagtaaat aaattaaatc tttgtgccag taaattatct
     4741 tgctcataaa gattatcttg ttcatgattt tctgaataat atacatcact cattttgttt
     4801 atttttctgt acattaatta tttttaaaat atattccaaa caattagaca tttacagacc
     4861 tcctttctgc ataagaatta tatctaatta tttggatata gttttaatta atcttgcttt
     4921 tttacacaat tatatttcta ctaaagaaac ttttaaatcc ttactttaat aattttatag
     4981 ctttcagtgc ctttagcgca aatacttgtt cacatagttt agtgtcatat gaaacgtatt
     5041 tatatatagt tatacattgt ctatttttac taatttatta actacttgca cttttataat
     5101 gtctcatacc taacttccaa aatctccaca taaacaatgt aaacaaaaat acaattaaaa
     5161 ttgagtatat taatccatta attgttattg tcccagccaa aatttgagtc ggaattgtcg
     5221 acataattcc atatggtaag attaaatgga ataatacttt aaaaacacct tcatatatta
     5281 caccaggaat tttcatattc attggcaata aattatcttc taatctcatt atcgcagaag
     5341 atgatataac aaaaaatgaa actgttctaa gtatgataca tacatcataa aaaagtaatg
     5401 tcatcaataa caccatgaaa atatatatta ttatcataac taaagttatc tctgagttta
     5461 actcagatat tccatataaa acaataagta tacttgctag aattaatggt gatgaaccaa
     5521 tattaatatt ttcaaatgtt aacctaaata gtgggtctaa tggttttgta atatagtagt
     5581 ctaattctcc ttcttggatt tttcgtggaa tatcataaat accaaaaaag aaaatcgtca
     5641 tatttatcgc atttattaat gagaaagttc ctataaatat taacatctct ccttgttgcc
     5701 atccccctat tgagtctaca tgagaatata tactattaaa cattagaagt tgtaatataa
     5761 ataatgaact atctacaaaa aatgctccaa aaaaatcgaa tctaaatacc atcatctttg
     5821 ataatttcat ttttaataac ataaatatta ttttcaaatt ttttttcatc atatacccac
     5881 cccatcaaat aatgttctca ttcttttata cataacttta tttactacag aaaaaaataa
     5941 aacccataat gctagaataa ttaatccttg tattgcctca ttgctgtttc tacctattag
     6001 taacatagat ggcaaatatg ttgtataata aaatggaaat accttcataa ctgttactat
     6061 agtttctggt aagagtacaa gtggtatcat tgttcctgta ataaattcaa caatattttc
     6121 aattatcatt ttaaaaaaac taatctcaag aaatttaaat gacaaaatcc caataaagaa
     6181 gtggagtttc atcataaaaa taaaccctag aaaaatcatc aaaattgaaa ttaatatttg
     6241 aactggattt gatgtaaagg caaaatctat tttaaatata aaaatccata caaatgcaga
     6301 aatcatatta aatacaaaat aaaaacatgt cattccaaaa tttttagcaa taaaataatt
     6361 ttgaacatta attggaataa tcatgtactt tgaaaaggtg cccaatttta cacgattaca
     6421 tatctcttca ctggtactct ttgacatatc aatacctgct aaaaatgagc ttattatata
     6481 gtaagacaac atagagttaa atgtaaaacc agagactatt ttattttcac caaaaattgc
     6541 tttccataaa atataagcaa ataaaatttt tacaatcgta aacaccatat taaccaccac
     6601 atcaaatctc cagataagtt gcactttaaa gttaattttg gctacttcat aataccttct
     6661 aatactctgt ttaaatttat aaaacattga atcatttgaa cttgcttcat tatagttgct
     6721 cataatactc atcacttcct ttcttatata ttctctctac aatactacct atatcttctt
     6781 cttcaatcat tacatcttta acatcatatt tatcaaaaat ccatttaatc gtttcatttc
     6841 ctctttcttt tggcacaatt actgatatct tatgatttgt ttgttcaaca actttacaat
     6901 cttttggaaa tacaatgtta gtttcattgg aaaaactaac tgttattttt ttatattttt
     6961 gaaaatgatt aaatagtttc tctgtagaac catcatatat tttacggcta ttttctacaa
     7021 caatacttct actacagaga ctcttaatat cttccatgta atgagaagta agtataatag
     7081 tagttccttt tgtttcattt acttctttta ggaaatacct aatttgtttt tgggctatag
     7141 catctaagcc tatagttggt tcatccaaaa ataatatttt tggattatgt agaagggcta
     7201 ctattagttc cattttcatt ctttctccta aagaaagcgt cctaacttga acatttaaat
     7261 agtcagatac ctcaaataat tcgataaagt aatccaagtt ttttttatat tcttcattag
     7321 atatatcata taattcttta aataaaagaa acgtatctgc tggtgtcaat tcaaagaata
     7381 gttgactttt ttggcccata acaacagcat attgtttttt aaaattattt tctaatttgt
     7441 tagggtaata acccattaca gatatttctc cagaagacgg tgcaataata ccagttaaca
     7501 tcttaataag agtagtttta ccagctccat tttgacctat taatccaaca aattctcctt
     7561 cttctatctt taaatcaaat gacaatatag ctgttttttc tgtatatttc ctattaaata
     7621 aagactttac actccccata aatcctgcct ttttctcaaa tcttctataa gctttagtca
     7681 gttcattaag ttcaattata ctcattttaa ctacctccat atttcataat ttatataaaa
     7741 aaagtccaca ggtatatatc tgcctttttc tgtgcttata tttaacagca caaaaacaga
     7801 aaactcctgt ggactagaaa aattaatgct ctctatttca aaatagttat caaaataata
     7861 ctatacaaaa ttaataaaag tgtagcagat taaagcctac tacaccataa tttaacaaag
     7921 taatatatat tttaaatact atttgttgtc atatgtcagt tatctataaa ggcagaataa
     7981 gacacaaaaa ttaaatgtat taaaaataca cttgaaattt ctgattatac agaatatatg
     8041 tgtctccaac tatttaattg ttggttacca ttatataact ccgacatcaa ggcactcctc
     8101 tcataatatt attattgata tcatatcata aaggttattt atgttcaagc atggattaat
     8161 atactaatac atttagctta tttcttcaag gttgaattta aatttcattt ataatattcg
     8221 tttataaatt aaaaatataa tacgtatatt aaaactggtc attttactgt aatattttta
     8281 ttgtaaaaga tatactttta cctatacagg atgtaatttc tttagttcta tattataata
     8341 atattgtaat aagagttcaa cttataatac attaaaatat aaactgtaaa tcttaagggg
     8401 gtaatctatg gaaaataata caatacaaaa acaattaaga gatttatcaa aaaaaatttg
     8461 gcatatttat attttaaatc cagaagaaga aaaccttaaa tttattattt ccttattaga
     8521 tgacaacctt gcattaattg gaacaggaaa acatgaattt tacgaaacat tagaagaatt
     8581 tattataggt cttgaaaaag accaaacaga atcagagcaa atagactttg agattataga
     8641 tgagtggtat tcatgtacca aagtcacaga cgaagctttt atagtatatg gtactctttg
     8701 ggtaatagaa aaagattacc tagaaaaaga tatctatgta gaaatggata ctcgatttac
     8761 tatggtatac agagttacag atgatatgat taaacttatc catgttcacc attctattcc
     8821 aaactttgac caaatgaaag atgagtatta ccctaaaaca ataactgaaa aagcacaaga
     8881 agcactagca atggcaaatg aatttaaact aaaatcagaa aaagacctta tgactggact
     8941 atacaataga catttttttg aagtacacat aaaagaaatg cttaaaactg tttcacatgg
     9001 atacttttac atctttgatt tagataattt taaacaggta aatgatatct ttggtcatgt
     9061 tcgaggagat gaaatactaa tacatttttc aaatattttg actaaagtat tcgtaaatga
     9121 tgctattatt ggaagacttg gtggagatga atttgctgtt tttgaatata gtaatcaatc
     9181 aaaagagaat gttgaaaata aggcaaagaa ggttttagaa ttatgcctag agagtaattc
     9241 ttgttctata ggtatatcta aattagaaaa taactctgat actttccagt ctttatacaa
     9301 acaatcggat tatgctttgt attattcaaa acgaaataat aaaggaaact ttagttggtt
     9361 ttagtacaaa agatattaaa ataggcaaat ttattagagt atcttgtcct atatactaat
     9421 aagtaaagta aacaacaagc tatctttgca tatatttata taaaccaact attcttaata
     9481 aaactacaat agtcaacatg accactatta tagaatataa taaactaaaa acatgattaa
     9541 tattatattt taataatttt atttttaaaa gtaagtccaa aatgttatat gttttgatta
     9601 aaactgatgt tgatataaaa aattttactt aaaaaaacac ttagaaaggc tttataaaga
     9661 gccttttttg cttttattga ttaaattttc atataaaatt ccttattaaa tttcaaatca
     9721 ataaaaaaag gctctataag aagcctcccc ctatttttat tgattaaatc ctaatataga
     9781 caatgcctta gcatcatagt aagtagtcat ctctcttgtt gcatccttta atttacggta
     9841 atcaaagtct aatgatccac caaaagctga tatttcagac ccatcaaacc tataccattt
     9901 cccatcaatt tgtatatagt tccaagcatg atttaatgat ggatttaata tagatagtga
     9961 atccattccc attaaccttg ttaataaatg aaagctactt gcaaaacttc cacatacacc
    10021 acgtttatca accagattac catagactat gttacaatat ttagagtctg catattttgc
    10081 ttcggttacc aattttgcag ctctattagc tctttccatc tcagatgcat ttctccagtc
    10141 aaagctattt aaaaaattcc tgattactgt aagtgtatct tgagcttgtt tgtaagaaat
    10201 ccaatttcca tcagctatta cttctatagg gtcaagtttt ccaaagccta ttctctgata
    10261 tgagccacct gacaatgctg ccgcataaaa tcctagccct tccataccct tatttatata
    10321 ataatattta ccatctgctc gaagctcaac tccattggga tttcctccat agagtaaatc
    10381 ccatccctct ttacctgttg tattaagacc aagttgtgtt aacattcctt taagaggata
    10441 tttagggtca taattagcac ctttacttat tgcatttgtt tgcactctgc catccattgt
    10501 gcctatatta gtctgattac gttcatttgc actctgtttt ttcaagctag atgtttcatc
    10561 ttcttgatat ggctctgcca ttactgtcat actcatcatc aatataataa tcatggtaat
    10621 catagtagtt aatatattct tcttcatact actccccctt atttaattct ttttaaaaca
    10681 taaaactatt aactcataaa tatatggtta ttagtaaatt gaaattaagt ctggttcata
    10741 aattattggt ttaatagaag tgtattgaaa ttgtaccttt atattttcat tatataataa
    10801 acttattttt ttaaaacata ttgtttaaat cagtacattt atatgttttt tttgttcatc
    10861 tgttttatca aatatattag tctttgaatt ttgtttgtat tcttcagcat ttttatctaa
    10921 cttttctatt ctcgactcaa gtttcattat ttcttcttgt gttaaattta taaaattttt
    10981 aagcatattt tcaagctctt cacgagtttt acctactaaa gataagtcct cagatacttt
    11041 ctctttttta ttagaatcct ctttataatc ctttttttgt tctaactctt ctaatttttc
    11101 atcatttaat atttcttctt caattttttc attagactct ttagtgatta aagattcttt
    11161 ttcatcattt gtatttagat aagttgaatt ttcatctaac attgtacttt tatttttctc
    11221 ttctataatg ctatttttaa tacctatttc tatattagaa aaattattag ttgtttctct
    11281 tatttctgat ttacataagt tcttaattgc gtcatttatt tttttagtat aaacctcttg
    11341 tctatatttt aaatctctaa ttctatcatt aagttcttgc ttttgtttca taatattaga
    11401 taaataagtg tcttttccta cagtttcatt tttcttaacc atactttcat ttattcttgt
    11461 attatttata ttactttcat atctaaatga atattgactt ctatattgac tagatatttt
    11521 catttatttc acctccttaa aataaattta tgtttataaa aaccaactta acttaattat
    11581 attttatcat caaaagtata catttacaaa tattagttaa tagttttata ggttcgagca
    11641 ttattttcaa caaaaaattt ttattataaa ttaaaacaat aatttttata aatatattat
    11701 ttgtatttta taattcatat atttttactt tataaattag caatattgca gttttgctct
    11761 taagaaacat ctctatcata cataagcata tactctatta tgtattagta taaatatgcc
    11821 ttttcttatc ttacataaga cctctttata aaggagccta tcgtaacaaa tatgtataca
    11881 ttatcacatg tatatatcat catataaaaa atcaaacttt attaaaaaaa ttaataaatt
    11941 gctttaaata ataccacaaa ccaaatttgc catcaaaatc acattaaagt aacaaattga
    12001 tggcaaattt aatatattaa tagtatatag actcatagaa tgaacaaaaa aactacactt
    12061 atcttctaat cttcttattt atttttgaga tatttttaaa tttcttttct ttttttgcta
    12121 agtattcgct tttattgtct gcaaatactg cttcagtttt aagcctacga taggattccc
    12181 atcttttagg cgaaagttct tgatgagcaa tagcttccca gatagcacag cctggttctg
    12241 aggtgtgagt acaattacga aaatgacatt ttccgaaata agcttctata tctgcaaaac
    12301 ttttatctaa accttctgaa acatcccaca ttcccaactc tctcatacct ggtgtgtcaa
    12361 taacaatacc accacctgaa attaaaaata gctctcgtct agtcgtggca tgtcttcctt
    12421 tatcatcatc tatgcgaatt tcctttgtat ttaaaaattc tccacccatc agacaattaa
    12481 ttaatgaaga ttttccaact cctgaagaac caagtaaggc aacagtacag cctggtttaa
    12541 tatacttaga aatctgttta taaccatctg gctctataga tgaggtaaca actacatcta
    12601 cacccaaggc tatagaatta actacttgta atttacgatg cacattgtca cataagtctg
    12661 atttagtaag tactactact gggatagctc cactttccca tgttaaagac agatacctct
    12721 ccaatctatg aatgttaaaa tccttattca gtgacatgca caaaaaaaca atgtcaatat
    12781 tagctgcaac cacctgctcc tcatttacct ttccagcagc cttacgcaca aacaagcttt
    12841 ttctatgaag cacatgatga ataatagcat taccattttt attattgcaa cgatccaaca
    12901 taacgaaatc accaactgtg ggaaaatcag attgtatagt agcctcaaaa cgcagctttc
    12961 ctgaaacctc tgcaaacagt acaccattta cacatacagc ttgataaaag ttcttttcct
    13021 gcgagataat ccgagcaagg tataaatcat gatattccat actttgagta ataaaatttc
    13081 tatttagacc atagtcttgt aaattgattt gtttcatacc attcattctg tttcctcctt
    13141 ttccaacttt tcaatgattc tttctagtgg gaaagggggc tgtgccagtg gttttaatgc
    13201 aattgacatt aatttcactg aattatcatc agctgcaata cgattagaac taagatgtcc
    13261 acagcgtgta caacgatgaa taattgccca ctcaccattt tttctcaccc acactccaat
    13321 aggttccata ttagcatcac aatctgcagc acggtcacca ggaatgatat ctacatgaag
    13381 actatgtaaa caatgtggac aatgatttct atgttgtgta ccagctcctt caggaactac
    13441 aaggcaacca caatttttgc aaataaagtt atccatacag gaattttcct gataataagc
    13501 tcgttttcta tttttataat tcatttttat ttcctccgta tcgtgtattt tctacaaaaa
    13561 aagtccacag gtatttatat ctgccttttt atgtggtata catatttacc tacacaaaaa
    13621 cagaaaaccc ctgtggacta gaaaatttag tatcttctat ttcaacataa ttatattcaa
    13681 aataatactg tgtaaaatta ataaaagtgt agcagattaa agcccactac accacaattt
    13741 aacaaagtaa tatgtaatat tattatttgt tgtctaatgt tagttatcta taaaggcaga
    13801 gtaagacaca acaatcaaat gtatcaaaaa tacacttaaa aattctgatt aaacagaacc
    13861 catatgcgtc tccaactatt tatttgttgg ttaccattat atataactct gacatcaggc
    13921 aagacactcc ttcactaata attattattg aaattttatc ataaaatata agtatgttca
    13981 agtataattt aatatattca tatatttatg tttgtctcat tttcttattc tataaaatct
    14041 agtggtattt tgaaatttaa tgttcaccta gtattaataa taaaaattaa ctgctattat
    14101 ttgttttctc attttatgcc tgttgctcaa tttatctcac ttaaattaat aacaactgtc
    14161 ttttcataca aaacctacaa tcaagttttt ccatatattc tacatgcata gtctattttt
    14221 tttgatataa aataacgttc atatttgtaa atttatttaa ttttcaaata agtatattga
    14281 ataataagca attctattgt attattgtaa ttgtagaata aaactaaatc ggcaaaacta
    14341 gagaaattta gtgacgcaaa gctataggga ctaacgctta taaaataaac tatgagttat
    14401 gtcagccagt tgccaaaaag agatattctt tttgttttta tgaaagtttt ttagggggat
    14461 aataatatat atgtttagag ataaaatgga tagatgtaca catatgttga ctgcttatat
    14521 tggtagttca tatgattatt gtgattttat agatacacaa ctagatgatt ttatactaga
    14581 gtacggagaa aatgtggtag aatcttgttt gcatcaagtg atggtattag taagtaagta
    14641 taattaaata gatttggttt tctagagaac cctttttagt tttatctact ttaaatagcc
    14701 ttatgttaga aagctaatta agatagattt ctagtattca taattttatt ttttatttaa
    14761 aatacatata ataaagatgt aaatttgtat caaataataa atatgagaac ttaaattctc
    14821 ttatctatgt gaaaataaac atctcttaaa atgaaattct gtaaatataa atgatttttt
    14881 agtttgaagg attagattaa caaaattata aatttgcaaa taagctatat taaaaaatat
    14941 aataattttt tatataactt attcttttat aacttttaat tttaaaagag gagaatgtat
    15001 ataagagtat tcaatgtact agaaaaaatc aaaagaaata ttagaagaaa tggatattga
    15061 gtaatcaaag aaaagatttt gatattacaa atgatatgtt tcaagaaagc attcctatca
    15121 caacaaaaat tattcttgaa gacatgcctt caaatgatga attaaatcat gttttttcaa
    15181 aaggttgtga acgaaaaatg aaaaagagaa aaattgtatt aatcacacta ctattaatag
    15241 gtgttttgtt attaggaagt atcctatata atcttttttt aggaaaaaca gctaatattt
    15301 caatgctaaa ggaatcttgg aattttgata taccaatacc taacaaagaa attgaagtat
    15361 ttgatactca agatagtatt aatggagatg gacaaagtta ttttattcaa ggattttctg
    15421 aaaaaaattt caaaaaagtt tttaatttaa aaggtggtat agttgtaagc aaagataata
    15481 ttaatgaaat agaaaaatat attgataaat ttaaaagaga ttctgttaac attaataaaa
    15541 gtaataaaaa taaaattgaa gaagatttta aaaaatataa gttagaagtt aaaaaagatg
    15601 ataagtatat atataagaga aattatgaga actacgtagt tttaattata aaaaaagatg
    15661 aacaaaaatt atacagttta atatggaatc agtaggtaca tatgtattat taagaattat
    15721 aaataataaa aataaatttt agtcaatata caaaaacctt ttaaattgtc tggaaatcac
    15781 ctcttcattt gttataaaaa catacaacaa atgaagaggt gattttatgg ttttaaaaaa
    15841 aataattata agtggatatg tgctttcaca tgtttatcaa catttgtaat gcctatattt
    15901 gctaatgaat tataaaaata ttaaataata ttcaactata gattatgtaa attactctat
    15961 gaaaaaaatg agttgtataa aatctggcta ctgtagattg actatttctt ttaaaattac
    16021 aattataata agttttatag aattaaaaat aaaactttag agatttttga taacattgta
    16081 gactaaattt gccaccgaaa tcgctttaca gtggcaaatc ggtggcaaat acaatatatt
    16141 attagtatat aatctatata aacattacta ttgtttgatt atcttagcta cagtttccac
    16201 atgtgtagaa tgagggaaca tatctacagg ttgtgcctca acacatttaa atccgtgaac
    16261 atttagataa tctaaatccc ttgctaaagt agaaggatta caagaaacat agacaattct
    16321 ttccggattc attgaaacaa tagtctctaa taccttttcg tcacatccct ttctaggtgg
    16381 gtctacaacg actacattgg cagtcttacc ctctctatac atcttaggta caatttcctc
    16441 tgcctttcca acataaaatt cagcattatg tatattgttt aatttagcat ttatcttagc
    16501 atcttttatg gcatcatcta taatttctat tccataaacc tttttagcct tttgtgccaa
    16561 aaataaagat attgtaccta tcccacagta aatatcaaaa acagtatcag tatcatttaa
    16621 atttgcatat tcaagagcct tatcatacaa tactttagtt tggacagggt ttacttggaa
    16681 aaatgataat ggtgaaatct caaacactaa gtctcctata taatcgttta tttttccatc
    16741 tccatatata actatatttt ctcttcctag aatagcattc gtatcttttt gatttacatt
    16801 tacaaccaaa gtcttaaatc caggtatatt ttccttaagc actgaagcta attctttaag
    16861 atatggaagt ttgtttccat ttgctactaa tacaaccatt acttctttag ttgtaaaacc
    16921 tatttttgtt actaaatgcc taatcaaccc cgtgtgagtc ttttcatcat atatgcttac
    16981 cttataggcc cttatatatg tttttattat tttaacaatt ttatcattta tctcatgttg
    17041 tattacgcat ttttctgtcg atattacatc atgagtcttc tttttataga agccaataat
    17101 tggaatacca ttattctttt gtattggaaa ttgtgcttta tttctatatc tatatggatt
    17161 atccattcct atagtgtcat gaacttctac atcctctaac ttacctattc tcgaaagtac
    17221 ttgtttaact tcatttgttt tcatttcaag ttgtttatta taatctaagt cttgtatctg
    17281 acaccctcca caatccctaa gattgtcact acacactctt ttcaccctga atggagactt
    17341 ttctattatc tcaacaatat ctcccacagc ataatttttc tttactttat taatcctaac
    17401 tttaacttta tcatgtatta atccgccctc tacaaaaact gtaataccat tgtactttcc
    17461 tactcctact ccgccttgac caatgtctac tatatctaca atatacatct cgtttttcga
    17521 aagcataaat caccctccaa tcaatctatt atatttaaat ctttacaaac ttttttcttt
    17581 aaatttatat taaaatattg ctattaatta aactatattt tttactaagc tacaatattt
    17641 tattttacca aaaaaaaagg agcttataaa gccccttagt aaataaattt attatttttc
    17701 ttataaaact ctagttgaac ctctatatac cacgcctctt gatgcatcca cagttacaac
    17761 ttctccatct tttactagat ttgttatatc agctgcagac acaataactg gtatatctaa
    17821 atttatacct acaatagctg tatgtgatgt cattccacca ttctcagtta ctacagctcc
    17881 tgctttttct atataattgt tcatttcagc atcagtagca gtagaaacaa gtatgtcgcc
    17941 ttcattaaag tctacacaag catttccatt ctttattata cgaactttac cttctactgt
    18001 ttgagtacct acacctatac cttgcacgat ttcttcgctt attacatgaa cctttattaa
    18061 attagtagtt ccacttactc ctactggtac tccagcagtt ataactacta attccccatt
    18121 atctatataa tcagcttgtc tagctgcctc aattgccttt tctataactt catctgtatt
    18181 tcctgcaaca gaagacttaa ctggatatac tccccatgta agagctaatt tattcatagt
    18241 cttttcatta tttgtagtag ctattattgg agattttggt ctgaatttag ataccattct
    18301 agcagtatat cctgaagaag tagatgttat tatagctgat gcatttaaat ctactgctgt
    18361 tgtacaagtt gcatggctta ttgcatctgt tacagtaaca ttgttagtac cattttcctt
    18421 tagtaatcta tcataatcta aagtctcttc tgttcttttt gctattgtag ccatcatttt
    18481 tactgcctct acaggatatt ttccagcagc agtttctcca gataacatta tcgcatctgt
    18541 tccatcatat atagcattgg caacgtctgt aacttctgct cttgttggtc ttggatttct
    18601 tatcatagaa tcaagcattt gagtggcagt aacaactggc tttgctaatt cattacattt
    18661 ttttatcatc attttttgaa ctattggcat ctcttcagtt ggtatctcaa cacctaagtc
    18721 acctctagcc accattatac catcagaaac ttttaatatt tcatctaggt tctctacacc
    18781 ttcttggttt tctattttag atattatttg tatatcagtt gcattattgt tctctaatac
    18841 ttctcttatt gctagtacat cagatgcctt tcttacaaaa gaagcagcta tataatctat
    18901 tccttgactt attccaaact ctatatcact tatatcttta ggagttattg ctggtaaatt
    18961 tatcttaaca ccaggtacat ttactccttt atgattttta actattcctg aattttcaac
    19021 aacacaaact atatcttctc catttatttc ttttactctt aagccaacta aaccatcatc
    19081 tattagtata gaatctcctg atttaacgtc ttcagcaaga cctttatagc ttactgtaca
    19141 catttcttta gtccccataa tatctttcat agttatagtg aacttttgcc cttcttctaa
    19201 aaatacctct gggtcttcaa agttacctgt tctaatttct ggacctttag tatctaataa
    19261 tatagcaact ggcttattta atttttctct aactttcttt gccatatcaa ttctttcttt
    19321 atgttcttca tgcgaaccgt gagaaaagtt aaatctacac acatttagac cattttgcat
    19381 aagttgagtt aaaacttcct cactttgtga tgctggacct agtgtacaaa caatttttgt
    19441 cttctttaca ttatttaaca tatttgctcc tcctgagata ttattccaca gatttatttt
    19501 taatactaaa acttatatag ataatatttt tgccatctca taagcttctt tatcaaatac
    19561 tttcttttga gctaaagctt cacttatttc taaatcaatt attttgtttt cttttattcc
    19621 tacaactcta gctgattttc catctaataa taattctact gctcttactc caagtctact
    19681 tgctagtatt ctatcagata cagttgggct tcctcctctt tggacatgcc ccaatacagt
    19741 gactcttaaa tctgctcctg actctttttt aagttccttt tctaaatcac tagcacttcc
    19801 gactccttct gctaaaacga ttatactatg tctcttacct cttttttgag tagtctttaa
    19861 tcttagagca acttcatcta ctgtaatttc tacttctggt actattatag tttccgctcc
    19921 tcctgcaaga cctgcatata aggctaaatc tccacagtgt cttcccataa cttctactat
    19981 gttaactctt tcatgagatg atgatgtatc tcttatctta cctatagcat ctataatagt
    20041 attcattgca gtatcaaatc ctatagtata atctgtatat gctaaatcgt tatctatagt
    20101 tcctggtatg cctatagctg ggaatcctaa gtcactaagt ttttgggctc ctgcaaatga
    20161 accatctcca ccaattacaa ctaagcaatc aattttatac tttttaagaa tttttactgc
    20221 ttttaatcta ccttcttctg ttttaaattc ttcacatctg gaagatttta atatagttcc
    20281 tcccctgtgt ataatatctc caacagaaga taagttcatt tcagttaaat cttcttctag
    20341 taatccctta tatcctctat tgattccata tactttgcat ccataatata ttgctgatct
    20401 tacaaccgct ctaatcgcag cattcattcc tggagcatct cctccacttg ttagtaatcc
    20461 tatagttttc attttttacc tccttggatt ttatgtcaat tttttattat aatcattgtt
    20521 attgtattct aatggacttt ttttgtccct atatcacttt ttaatcccat caagactaaa
    20581 aaaattaaat gtccttaaat gtccattttg tgatattata tcacaaaacg gacaatattt
    20641 ttcatattat tataatatat ttagattttt atataaaaaa tcaagctttt acttttatgt
    20701 tatctttagg aagtaactct tctaagttat ttataaattc atcacatata caagtcaatg
    20761 ccccggtcat tttatatgct ttacgtttac catttatatt ttcatccata gggtataagt
    20821 atatactgtc attacctgga tattgtttgg ctatttctac aatattatta gttgtatcag
    20881 tatcagacat actatctatt ttcaaataca attttttacc actttctggc ttaatatttt
    20941 ctacatttct agttcttcct gaattgtagt ttctatctgc aaagtcagta tcatcattta
    21001 tatcttttat ctctctagct ataagttttg cattttcgcc ttcttttata cttaatgttc
    21061 cttttacata tattatatta tcttcattca aaatgacatt atacttttgt aataattgag
    21121 gaaaaactat tatttctata gcaccataca aatcttctaa ttcaataaat gccataattt
    21181 catttcgttt tgttgtttta atagttttag ttacaatcat tccacccatt ataacatctg
    21241 tttcattcat ttgtaggtat gactcttctt cctcttttaa tgaatttaat tttccattat
    21301 ccatagatgt tttttcttgt agttcttttt caaattgtga taaaggatgt ccacttacat
    21361 acattcccaa gacttctttt tcaaggttaa gtctctctct ttcttcgtac tcttgccttt
    21421 taggcaaact gtatatttgt tgaaaatcac tatcttcctc actatctcca aaagcatcaa
    21481 ataaagaaat ttgaccttga acatttttct ttctatccat agatacactt tctaataaac
    21541 tttcataacc agccatcaga ctagctctat tttcacttat attatcaaaa gctccacatt
    21601 ttattagact ttctattact ctcttatttg tatctttttg gtctaatctt ttgactaaat
    21661 ctactaaatc tacaaataat ccattagatt ctctttcagc gattatattg tttattatat
    21721 ttacgccaac attttttacg gctgctagac caaatcttat attgtcgcct tcaactgaaa
    21781 actttgaaaa acttttgttt atatctggag gtaatacatc tatttctagt gctttacatt
    21841 ctcttatata ctcaactacc ttatcagtat ttcccattac actggttatc aatgctgcca
    21901 taaattcaac tggataatat gtttttagat aagctgtctc atatgctaaa accccatatg
    21961 cagctgcatg tgatttatta aatgcatatc tagcgaagtc tatcatatca tcaaatattt
    22021 tattagctat ttcctcttct acaccatttc tgatacaccc tgcaatttct atattaccat
    22081 cttcatcaaa tttaccatga ataaaatatt gtctttcttc ttccatcacg tccatctttt
    22141 tcttactcat ggctcttcta actaggtcac ttcgtccgta actataacca cctaaatctc
    22201 taacgacctg cataacctgc tcttgatata ccaaacaacc atatgtaact tccattatag
    22261 gctttaattt ttcatgtaga tatgttacat cttttgggtt gcttttattt tttatatatg
    22321 caggtataga atccattgga cctggtctaa atagggatat tcccgctaca atatcttcaa
    22381 aattgtctgg ttttagctgt ttcataaaac ttctcatgcc agcactctcc aattgaaaca
    22441 cccctagtgt atttcctgat gaaagcattt catatacttt cgggtcgtca taatccatct
    22501 ttgaaaagtc tattttttct gtgtaatttt ttataactct atttttttct attaaatcaa
    22561 gagtatctct tataactgta agagttctta gcccaagaaa atccatcttt aaaagcccaa
    22621 gttcttcaag tgttgtcata gtaaactgtg ttgtgattgc atcttgatgt ttgtacaaag
    22681 gtacatattc atctacggga ttttttgata taacaactcc tgctgcatga gtagaagcat
    22741 gtcttagcat tccctctatc tgttttgata tatttattat ttcttttgtt tccgggtctt
    22801 ggtcatacaa ttctttaaga ttaggatttg tatctagggc cttatctatt gtcattccta
    22861 aagcaaatgg tatttccttg gctaccttgt caactttatt gtagcctata tccaataccc
    22921 ttccaacatc tcttattgca gcttttgcac ccattgttcc aaaagttatt atctgggcta
    22981 catggtcatc accatatttt ctctttacat aatctataac ttcttctctt ctctcataac
    23041 aaaaatctat atctatatct ggcatggata tacgttctgg atttaaaaaa cgctcaaata
    23101 atagagagta ctttattggg tctatatcag ttattttaag tgtatatgct actatcgaac
    23161 cagccgcact acctctacct ggtcctacta taatattatt ttctttagaa aaatttataa
    23221 aatcccaaac aattaaaaaa tactctacat aacccatttt ttcaattaca tctaattcat
    23281 aatttaatct ctctagtatc tcattactag gattatcata tctctctttt aatccattaa
    23341 aacacaactc ccttaaataa gtttctggtg tatatccttc tggaacatca tacttaggaa
    23401 gatgtatagt attaaaatca aactccacat tacacatatc agctattttc acagtattgt
    23461 caatagcctc taatgcatat ggaaataatt cttccatttc ttctcttgat tttaaataaa
    23521 actcatcact accaaatctc attctatttg ggtcatttaa tgtttttccc atttgaatac
    23581 acataagtac atcatgtatt ttcgaatctt ctttatttac atagtgaaca tcattagtag
    23641 caactagtgg tataccagtc tcttttgata attttactaa accagagttt acttcttttt
    23701 gctcaggtaa attgtgatct tgaatctcca aaaagaaatt ttcttctcca aatatgtctc
    23761 tgtagtcaag agcaaattgt tttgcttttt cataatttct attcattaaa gcacgtgcta
    23821 catctcctgc aaggcatgca gaaagtgcta taatcccttt gttatgtttt ttaagttctt
    23881 ccatgtcaac tctaggctta tagtaaaaac cttctacgta tgaagttgaa actattttta
    23941 ttaaattttt atatccttcc atattcttag ctaacagaac taaatgtcct tgatatttgt
    24001 catagtttgg gtctttatct ctcaatccac gtgcagctgt atagacttca caccctataa
    24061 ttggctttat tccttctttt atagctgttt tgtagaaatc tataacacca aacatacaac
    24121 catggtcagt tatagctatt gagctcatgt ttagttcctt agcccttttt ataagttttt
    24181 tcaccctcga aaacccatca agtaaactat actcagtatg tacatgaaga tgagcaaaat
    24241 ctttgttcat taaatcacct cctgctttat aattttaatt ttaccacaaa tatatgtcct
    24301 aaaacaaaaa cagagacttt tatctcaaaa taggaattaa cttcaacgta taccattata
    24361 tgttataaat taatgcttat tctgagattg tcccctcaat ttattgctat gatttttttt
    24421 tcaaatctgt tgcaatttct tctatttttc taagtctatg attgactcca gattttccta
    24481 ttggtggttc taacatctct ccaagttctt tgagagtcat atcctcatac tcgattctaa
    24541 tcttagctat ctcttgtaaa ttttctggca aactgctaat tcctatagtc tcttgtatga
    24601 gcttaatatt ttctacttgt cttactgctg catttactgt tttagaaagg ttagctgtct
    24661 cacaatttac tatcctgttt acattatttc tcatttcttt tactatttta gtattttgaa
    24721 gacttaaaag agcgtggtgt cctcctataa tactcaacaa atctgatatt tgttcactct
    24781 ctttgatata aacaacgtga ttattttttc tagccacaat tttactatta aaacctaaag
    24841 agtttataag ttcttttaga gaatctgcaa actcctcatt attagttaca aattccatat
    24901 gatagttctt ttctgggtcg cttatagaac ctccaccaag aaaagctcct cttatgaaag
    24961 ctctcttaca tccttcatgc ttaattaagt tctctgaaat tttgtttata gtaaaaaatc
    25021 cttctttatc ttcaagtata cctaactttt ttaataacat ttctgaaccc atttcgcttg
    25081 ttaccattaa tacataactg ttatttctct ttagcatttg attcttattt actgagattg
    25141 tagtattgat attaaaattc ttttttaata atttaaatac tttacgggct attgaattta
    25201 actctgttgt tatctttaga tttagcttct tatatccaac aatttgtata cttccccctg
    25261 atttaactag agctgacaat tcagctatat tacaacattc attcccagat ataactcttg
    25321 ctagttcatt tttggtttca gttgaaaaag acatatctat cacctattaa ctaatttttc
    25381 tttaaccttt tttttatttc ttgtaaggca acctttatta tctccttttc atcatcatac
    25441 ctagctaagt ctactactct atctaggtgg ataaatttag catcaataaa gccttcctct
    25501 ttttgtggaa tagtatccat attctttgct tgcatcaaat accaaaaaac tgttttatga
    25561 actgctctat tttcatccca gttttctttg aatgtatagt gtatttcacc taaatatttc
    25621 aatatatctg cttttactcc agtttcttca cttacttcac gtaatgctgc ctcttggtta
    25681 ttttcccctt cttctacttt tcccttgggt aacacccaat ctccattgaa cttccttagt
    25741 aaaagaatcg tattgccaaa caggactaca ccaccagcac taacctcttc tctcatcgat
    25801 gttcactcct taaaactaac ataatgataa ggatttctac acaatttttt aaaatccttt
    25861 tttactaact tttgttgtaa ttatgattta gggctaactc aataactatg ttagatatat
    25921 actttgcatc atgtcttata taattatttt ttatttcaat taaattcttc tctacagttt
    25981 ttatacccat ctcttttaat ttatcttttt gctttttatc taacaagact tgctctgctc
    26041 catcttgttt atataagtca agcatttctt ctggcaatat ctcattattt gcgattacat
    26101 aatctattaa attctcatca gtgtgtttta ctatagcatt aacatgctct aatacattgt
    26161 acccttctgt ctcacctggt tgagtcatta tatttgctat atatactttt ggagctacag
    26221 acgatttaat agcgtctaca atcccctcta caagtaaatt tggtattata cttgtatata
    26281 aacttccagg acccataatt ataatgtcag catcataaat agatgcaata acatccttta
    26341 atggttttac atcttttgga tttaaaaaaa ttttatcaat agagcttttt tgaatttttg
    26401 actcttcagg tatactagat tctccattta ttatattgcc attttctaat ttggcaacta
    26461 aatttacatc ttccaaagtt actggtaaaa ctcttcctgt tatagcaaaa atttcactta
    26521 atttatatac agctttttca aagtttccat acaacccatt catagcagcc aaaaacaaat
    26581 ttccaaaact ctgacctttt aaaagccctt ctgtaaacct atattgcata acttcattca
    26641 ttgttggctc tatatttgca agtgctaata aacagtttct tatgtctcct ggtgggagca
    26701 ttcctaagtc ttctctcaat acacctgaac ctccaccatc atcagcaaca gttacaattg
    26761 cagttatatt ttgcgtagtg tgtttaagac ctcttaaaaa taccgattga ccagtacctc
    26821 cacctatcac aaccacattt ggttctctat tttcaacaat agacctttga aatagtttta
    26881 ctttttcatt ttttgctttt ttatatataa ataacgatac aattaatgca atccaaccta
    26941 aaattcctac aataataagt aatttagcca tgtttgcctc cattatcgca acatagagtc
    27001 tctatgtttc tttacaactc tatatccctt attagataaa tcctccgcta taagatttgt
    27061 tatagtaact gacctatgtc ttccacctgt gcatccaaca ccaattacta gatgttgttt
    27121 tccttcctct atatattgag gtactaaaaa atgaatcata tctaaaagtt tcacataaaa
    27181 ttcttcactg attttagaat tcataacata atctctaact tctttatcat caccagtttt
    27241 agctcttaac tcttcaacat aatagggatt tggaagaaat cttacatcaa atactaaatc
    27301 tgcatcagca agtataccat gtttaaatcc aaaagatact acagaaatag ttaggtttgg
    27361 attatcttca ccagaagaat atattttctt tatttcttcc ttaaggtctt taggtttcat
    27421 gttagttgta tcaattatac atgtagataa ctcctttaaa ggttccatta tctttctttc
    27481 catttttata ccttctggaa tctgcatatc ttttgccaat ggatgatttc ttcttgtcat
    27541 tttatatctc tttagcaata catcatcatt acaatccaaa tataccattt catattcata
    27601 attttctttt tctaaataat ttaagctttc atgaagcgct tcaaaaaact ttctccctct
    27661 tatatctatc cctagagcaa ctttatcaat acttgaattt ggttgataac ataattctgc
    27721 aaatttagtt atcaaagcag gaggtagatt atctacacaa taaaatccca tatcctctaa
    27781 agctctcatt gtctcacttt ttccagatcc tgaaagacct gtaactataa caaatttcat
    27841 tgctaactcc tcccttttat cttatgttta ttatattata atgttttcta ctaacgatat
    27901 atccagatta gagtcagata tagtcttcat agctagttca aaatcaccaa ctctttctgg
    27961 acaatgtgca tctgagccta ttataaattt attttctagc ccttttattt tatttaattg
    28021 atttacattc aaaaaagaat ggctactatt tatctcaagt ctagtatttg tcttttttgc
    28081 tgtctttgct acttcttcta tatacacatc gcctttatct cctggatgag taattataaa
    28141 aatatcatta ttacttatcg catttattac agcttttgta ttatattctt ttgctttttc
    28201 agttttaaaa atatagttat tacaatgatt taataagctt cttaatgatg ttggagtaga
    28261 accaaaatga tatccagcca taatataatc aaaatctcta ataaatttat catccatatc
    28321 tatatttcct ttatcatcta atatgttaca ctccatacct aaaagtatat ttatatcagt
    28381 gtacttatca tttaaataat ctacttcttc tctcattttg agaatatctt ttttctttac
    28441 accatatgca atatgtttat aactatggtc agatattcct attgttttta ttcctttaga
    28501 aatggctacc tttacattat cctcaatagt ccctttgcca tgactatata atgtatgtgt
    28561 atgataatca gccagtattt tcatttgtta atcctctcca agtattttga cttcttcttc
    28621 caacataacc ccaaacttgg catatacagc actttttata acatatatta aatccaaaat
    28681 atctttagca cttgcttctc cttgattgac tacaaatcca caatgcttct cagatacttg
    28741 tgctcctctt aaagtaagac ctctcaatcc agtttcttct attaatttac ctgcaaaata
    28801 tcctgttggt ctcttaaatg tacttcctgc actagcaaaa tttaatggct gtttagttat
    28861 tcttctcgtc gctaaatcat ccattaaact ttttatttca tcataatttc cttcttgtaa
    28921 ttctaattct gctgataaag ctatatatcc gtttttcgat attatacttt ttctataccc
    28981 aaattccatc tgttcatttg ataattcaaa tatattgcct tccatatcca tcagtcttac
    29041 tgacttcact atatccttca tttcaccacc atatgctcca gcattcatgg ctaatgctcc
    29101 tcccaatgtg ccaggtattc ctgatgcaaa ctcaaaccct tttaattctt gcttttggag
    29161 agctttccct aaaactgata ataaagctcc tgactgagca gtcatcctag tatcatttat
    29221 ctcataagaa ttgaaattat cagctaattc tattaccact cctcttattc caccatcttt
    29281 aattaaaatg tttgaaccat ttccaattat taaatacgga atagactctt tttttaccaa
    29341 cttaagtaca ttttttaatt gttcttcagt tctaggtcta actaatatat ctgctggtcc
    29401 tcctacccta aatgaaatat gttttttcat aggttcatcg acttttatat cctctttact
    29461 taatatattt aataaattta tatatatatc ttgattattc attgtatttc ctcccaaatt
    29521 atactgaact ttagctgtca ttattattta aattacatta atatatagta ttaactatat
    29581 tcaataatat atgattttgc ttatcatatg gattttatat tttaagcttc ttaattacaa
    29641 actatattca ataaatatat aataaagtaa aaaaaaaaaa ataacaacat ataaaagaat
    29701 ataaataatt taaaaaaagc atcaatatca ctgatatcaa tgctttaaaa ggcaatttaa
    29761 ttcgtattat ttatctaact atttttgaca tagcaaagat aataaatccc tccaactact
    29821 atggaacctc caacgatatt tccaagtgtt acaaatacca aattagttat aaattgaacc
    29881 atagttatat ttgctcctaa tgccattccc attggtataa aaaacatgtt tgcaacacta
    29941 tgttggaatc cacataatac aaatagcatt attactgtcc aacatgaagc caattttgac
    30001 attatatctt gagatgctgt agccatccat acagctaaaa cgactagaat attacaaagt
    30061 attgctttta aaaacattga tgctatagtt gaattaacct tagcttctgc cacagctata
    30121 gcttttgtag ctgcatctcc tgtcaatgta gaagaataaa acacaagtgc aactagcaaa
    30181 attgaaccta ctaaatttgc aaaataaacc agtccccagt ttcttaacat ttttcctaat
    30241 gttatttttt tgtccataag tgcaatagcc attaaagtgt ttccagtgaa taattctgct
    30301 cctgccacta ctacaagcat aagacctact ggaaagacca tagcacttaa aaagctagct
    30361 acccctgggt ctatcttgcc aaaagtttga ctaactatca tactccccaa tgccccaaaa
    30421 cctataaatg ctcctccaaa taaacctaat agaattgcat tttgtgtcga aatatttgct
    30481 tttttaatgc ctgcacttat agttgcttgt gcagtttccc caggtgtcaa gaatccttta
    30541 ctcataaatc cttccccctt aaaaattata ctttcccttg tctttttatg gcaataatta
    30601 tataataaca atatgatata taataaattt tattaacata aaaaatatac tatatactta
    30661 tatatacttg tctcatataa ttatactatt tattatataa caactttgtt aatttcgaaa
    30721 taatttatat tgaatttgaa aaatttacct tttgattatt tattttactt tttaattatt
    30781 gaggtattat atataataga tatatgtttg tgttattata tataattact tattttaata
    30841 aatatttgaa ggagggaatt ttatgtttga tatatcattt ttcgaaatgt caatatatga
    30901 aatagcagtt actacaatat atataattaa tttcatggtt atactaaacc ttatctttag
    30961 agaaaaaaga aacataaata caactttaac atggcttctt atattagtat tgattcctgc
    31021 tcttggattc atactttata tagcttttgg cagaaatatt tcaaaaaaca atatgtttag
    31081 attgaaagaa aaggatgata agataatcaa aagtaatata ctagatactc aggttaaact
    31141 ccaatctaca tctgaaattg attcagatat acatcaacat aaggatatga tttatgcatt
    31201 ggctaattca aataatgctc attataccaa taataacgat gtgtggattt atgctgaatc
    31261 tagtcaattt tttaacagtc ttcttgaaga acttaaaaaa gctaaaaaat atataaacat
    31321 acaattttac attttcaaag atgacaaaat cggaactgaa ataatagaca ttcttataga
    31381 caaagctaaa gaaggtgttg aagtaagact tttatttgat gcagttggtg gtagaacact
    31441 aaaaaatagt acactttcca gacttaaaga aagtggtgtt aaagttggaa gcttcttccc
    31501 atcattcttg aaaattgtaa actttaactt aaactataga aatcaccgaa aaattgttgt
    31561 cattgatggt aaggtaggat ttgtaggtgg atataatgtt ggtgatgaat atttaggtag
    31621 aaatccaaaa tttggtctat ggagagatac acatactaag cttactggtg attgcgtaat
    31681 tgacctaaat atgagattca tccttgattg gagatataca actaaagaag acttagattt
    31741 agaaaaatac tttatagaag aagatacaaa ttctaatcat ccttctgaaa atattgggat
    31801 tcagatagta tcaagtggtc cagatatatc agaacttgat gagattaaat atggttacct
    31861 taaaatgatt cagaaagcta gaaagtatat atacatacag agtccttatt taatacttga
    31921 ctcaactttt atagatactt taaaaatagc ctgtttatct ggtgttgatg tgagagtgat
    31981 gattccttct aaacctgacc atccctttgt atactgggca tcatattcat atgctggaga
    32041 acttttaaag tttggagcta aaatctatac ttatggtcaa aatgcatttt tacatgctaa
    32101 gacaatagtt atagatgact ctatatgctc tataggaaca gctaatatgg atatcagaag
    32161 ttttgagctt aatttcgaag ttaatgcatt tatgtactct tcaaaaaaag cattggaaca
    32221 aagaattata tttgaaaatg atattttaaa ttctaaggaa ataacattag atatatataa
    32281 ttctaggtct acttatataa aaatcaagga atcaatttca agactattat cagcagtttt
    32341 ataaatcttt tattttataa aaattttaca ttttggttta ttttttaaag ttttttatat
    32401 gtattatttt ttttttattg aatttagctc atttttttta ttttttttaa ctattctgaa
    32461 ttattttata ttataataga ctagtatagt ttttattttt aataggtaac ttttttgcat
    32521 tgtttttata aataaagaaa ttgttgtata ttattctttc tttattgttt tggttttgtt
    32581 cacccacata atgttggggt caactcatgg ttaattattt tatgaaaaat aataacttta
    32641 aaaagaaaag ggggatttgt tatgtgcgat tttatttcac acaacaagca attatttgac
    32701 gaactagata tatttattga ctctcttgca acaaaggaag gtgcgttaat acaagttcta
    32761 catgaagccc aaggtatatt tggatatctt ccaaaagaag ttcaacttca tgttgctaga
    32821 aaacttggag tagctcctgc aaaggtatat ggggttgtta cattctattc ttattttact
    32881 acagaacctg taggtaagta taaaatcagt gtctgtctag gaactgtatg ttttgtaaaa
    32941 ggtgctgaca aaatcttaag tgcttttgaa aaacagcttg gcataaaagt tggagaaact
    33001 actagtgatt ttaaattttc tttagaaggt ttaagatgtt tgggggcatg tggtttagct
    33061 ccagttgtta ctgtaaatgg taaagtttat ggtaaagtga aacctgacca agtaagcgaa
    33121 atattagata cttatagaga attagaattg aattgttaag aggggtgaat aaataatgcg
    33181 taaagtaaat tcatttgatg aattaaaagt tttagctgat gagctgaaac ctaatcttag
    33241 cttaagaaaa aattattcta aggaaaatgt attaagaaga gaattacttg tttgttgtga
    33301 tactggatgt acatcttcaa acagtctaga aatagttagt gaattagaaa atgaaataaa
    33361 gaaatcagga atccaagata aagttagtgt tcgtttgaca ggttgttttg gtttctgtgc
    33421 tcaaggtcct attgtaaaag tttatcctga taacgttttc tacgtaaaag ttgagccatc
    33481 tgatgctgaa aagatagttc aaagccactt aattagaaat acagttgtcg aggaattatt
    33541 atatgaggaa gaatctttaa gtaaaaaagt tgaagaacaa gaagaaatgt ctttctacaa
    33601 aaaacagtta cgtatagctt taaaaaattg tggtcttata gacctagata gtatagaaga
    33661 atatattgcc aatgatggtt atttagctct aggcaaatgt ttaacttctc ttactcctca
    33721 agaagttatt gcagaggtaa aaacttctgg ccttcgtggt agaggtggtg caggtttccc
    33781 tactggctct aaatgggaag ctgcttcaaa aaatccagct ggtccaacag ataaaaagtt
    33841 tgttgtatgt aatgctgatg aaggtgaccc aggtgcattt atggatagat ctgtacttga
    33901 aggtgaccct catagtgtac tagaagctat ggcaatatgt ggatacgcta taggttctga
    33961 tacaggatac atatacataa gagcagaata tccaaaatct atagaaagat taaaagtagc
    34021 catagctcaa gctgaaaaat acggtttact tggcgaaaat atcttaggca ctggatttaa
    34081 ctttaaatta gaattaaaat atggtgcagg tgctttcgtt tgtggtgaag gtacagcgct
    34141 aatgcattca attgaaggta gacgtggtga acctagaatg aaaacttata gttcttctaa
    34201 gagtggtcta tggaaatctc ctacttgctt aaataacgtt gagacttttg ctaacatacc
    34261 tgcaattata cttaagggtg gagattggtt tgccaactta ggtactgaag attctagtgg
    34321 tactaaagtc tttgctcttg gtggaaaggt agaaaatgta ggacttgttg aagttccaat
    34381 gggtactact ttaagagata ttgtatttga aattggtggg ggaatccctg aaggtaagaa
    34441 atttaaggct gttcaaacag gtggaccatc tggtggatgt ataccaactg aacatttaga
    34501 cactcctata gactttggtt cattaagttc tatcggttca atgatgggct ctggtggtat
    34561 gcttgttctt gatgaatctg actgtatggt agatatagcc aagttcttcc ttgagtttac
    34621 tgttgaagaa tcttgtggta aatgtacacc ttgtcgtata ggtactacaa gattattaga
    34681 aatattaact aaaattacag aaggtaatgg tactctacaa gatttagatg acctagaaaa
    34741 ccttgctgaa actatacaaa ctgcttcatt atgtggtctt ggtaaagctg ctccaaaccc
    34801 tgttttaagt actcttcaat atttcaaaga tgagtatata gctcatgttg tagataagaa
    34861 atgtcctgct ggtaaatgtc agaacttact ttcttacttt ataacagatg actgtaaggg
    34921 ttgtacaaaa tgttctagag tgtgtcctgc tggttgtata acaggttctg ttaaggaaca
    34981 acatacaata gatacttcta agtgtctaaa atgtggtgct tgtatagata attgtacatt
    35041 taacgctata atcaagaaat aacttatttg gaggaggaat aacaatgagt ttagttaatt
    35101 taactattaa tggcaaacac gtttcagcac ctagtggaac ttctatctta gatgctgcga
    35161 aattaataaa tataaaaata cacaaccttt gtcatttaca tatgaatgaa atagataagc
    35221 ttgacacttg tgcttcttgt cgtgtttgta tggttgagac agagagagga ttagttccag
    35281 cttgtggaac agttataaag gaaggtatga aagttcagac taatagtgct aaagcattaa
    35341 atgctcgtag aactattgtt gaattattgt tatcagacca tccacaagat tgttttgtct
    35401 gtgaaaaaaa tggagattgt gaattacaga ctatagctgc tgatttagga gtaagaaaaa
    35461 taagatatca aggttctaaa tcttttgcag gtaaagatac ttctactaaa tctttagtta
    35521 aagaccattc taagtgtata ttatgtagaa gatgtgagac tgtatgtaat gacatacaaa
    35581 cagtaggagc attatctgga gttaatcgtg gatttaatac tttagttagt acattcttta
    35641 actctgatat ggttgaaaca gagtgtacat tctgtggtca atgtatatct gtctgtccaa
    35701 ctggagcatt aacagaagta gataatgtac ctaaactttg ggatgtttta aataaaaaag
    35761 aaaagactat agtagtgcaa gttgctcctg ctgtaagagt tgctattggt gaagagtttg
    35821 gattagaacc agggtcaatc tctacaggga aaatggtagc tgctttaaaa gcacttggat
    35881 ttgaacatgt atttgataca aactttggtg cagattttac tataatggaa gaagctactg
    35941 aatttataga aagaatccaa aaaggtgaaa atcttcctat attaacaagt tgttgtcctg
    36001 catgggtaaa cttcttagag cacaattatc cagacaagtt aaacttagca tctagctgta
    36061 agtctcctca aggtatgttt ggttctatag ctaaaaacta ctatgcacct aaaatattag
    36121 gaatcaatcc tgaagaactt tatgttgtat ctgtaatgcc ttgtgtagct aaaaaatatg
    36181 aagcttcaag agaagaatta agtgaatctg gaatactaga tgttgacctt tctataacta
    36241 ctagagagtt agctaagatg ataaaagaag ctgcaatcga tttacctaat cttcaagacc
    36301 aagattttga taatcctcta ggaaaatcta caggtgctgc atctatattt ggagcaagtg
    36361 gtggagtttt agaagctgcc ctacgtactt catatgagaa gataactaat aagactttag
    36421 ataatgtaaa cttcactaat gttagaggtc ttaaaggaat tagggaagca agtatagatg
    36481 ttgatggaac tacagttaat gtatgtattg taaatacact taaaaacgct agaaagatta
    36541 tggataaggt cagaagtgga gaatgtaaat accatatcat agaagttatg gcatgtcctg
    36601 gtggatgtgt tggaggtgct ggtcaacctt atcaccatgg taatactgaa atagttgata
    36661 gacgtgcaaa tgcgttatat gaaatagata gaaataaggc tattcgtaaa tctcatgaga
    36721 atcctgattt acaagctatt tataaagact tctttggaga accaaatagt gatgtagctc
    36781 ataaatacct tcatactcat tactttgata agagttgtgt ctatggagag tgtccacaag
    36841 aatgtgcttg tgaagaagct aagtagtcat aacaaagtat aaaatttatt cttaaaaaag
    36901 gattgtctta aaatactttt acgtaattta agacaatcct tattaatttc acaaattaaa
    36961 ttatatttct aaatagactt atttattaat taacctatct attaattaaa gaatacctgc
    37021 cattctcata agttgttttg catttgtagt tttacatctt ccatctttta tcttatagtc
    37081 aaaatgaatt ttatcgtcta catagtactc tgagaaatga tagtttacaa cttcaacatc
    37141 tccgctacct gaaaggtcac atagctcaaa gtcatgtgtt gtcaccatac ttataaccca
    37201 gggttttgat aatttcttta ctgcctcact tgctccaata attctatcag cagagttagt
    37261 tcccttaaaa atttcatcta ctagaaccaa cattggcagt tcttttaaac tatattgaat
    37321 cattgatttt attctaagaa tctcagcata gaaagttgat attccttgac ttacatcatc
    37381 ttgtattctc atagaagtaa atatagccat acaggtcgca tcaaaactct tagcgcatac
    37441 tggagcacct gcatatgcca gcactaagtt tacaccgata cttcttaaaa atgttgtctt
    37501 tccagacata tttgaacctg tgataataca agtctgagaa tttaattgta tcgaatttgc
    37561 tatgacactt ttttctgcta ttaatgggtg gtatacctct tcaactttca atcttggagt
    37621 atcttcatat tttattactg gaaaacatgt attttccttt acatgagaaa caactgttaa
    37681 acttattaga gcttcaaatt ctccaataga ttcaaaccaa tctctaattt gatttccata
    37741 tatacctttc catcgctcca aagcttctgc acaatgatag ttccacatca aaacaccaca
    37801 ggcaattata tatagtatct gattatacct taggtttaca gcatttccta ttgaatttaa
    37861 ttgtttaaca cctcttgaaa ctccacttcc cttagaaagt ttatcttgta attctttcaa
    37921 atatgcactc tcaaacgtct ctttttctaa aacctcaaac attttttcat atacttgtat
    37981 acttctacta aatgaaaata atggagcaag agtttgcata agtcttggat atccaaaacc
    38041 actaaaccct aactggatta taaaaagtaa aaaaacaggt aaaacaggta aaaatcctaa
    38101 catacaaaat ataaaagata agattgttgc agttggtaaa ccccaagtaa aaatatgcat
    38161 ccattttggt atatacacat tcttattttc tccatattct ataaacgatt ctatactact
    38221 atctgaattg tttttttgct ctttaccaat tatattactg agtgtttgaa tatgaataga
    38281 aaaatcatct tttgataaaa gttcttttat agctctctgt ctttctaaaa ttatattctc
    38341 atctggattt tcattccaca aatatttggc taaaaaattt ttcccataag atgtattagc
    38401 tacacagata tattgataca atgatgctct tccaaataag tctaaatctt ttgcttgcga
    38461 attttcatct tttaagtatt ctttaccatc tattttaaat tccttccaac catctccaaa
    38521 tctcttaata tacttattta atacttcgct cttactttta aaatatgttt cttcttcttt
    38581 tactttacta tgaataacta agagtgctat aaatattatt attgataaaa ctccaacaaa
    38641 aatacctact atatttttat tataaaatcc aatagcaagc cctgctatca ctatcaaaaa
    38701 agatattaat cttgctgtac ttatgttatt tgacttttta cttaagagca attctttttc
    38761 agtactactt tctaaaagtt tactaaaata ttctattccc ataaaaaatt ccaactttct
    38821 tgttatataa tgaaactcaa atataaaatt aatctagtat tattataatc taacactgta
    38881 ctttaaatta tccttatata atatatctta ttaataaaca ttatatatac aacttgattt
    38941 taatttataa ggtgtaggac ataatcctaa tcaatattta attaaatgat gtgtagtcca
    39001 ctattattca gtactactta gtatcgatta attttgataa ttttttagta aattcttgag
    39061 ctgcatcgta tcccattcct ctttgtctaa aattcattgc tgctacttct aatatcatag
    39121 cagtatttct tccaggttta acaggtacaa ctaatttctc tacattaaca ccaagtattt
    39181 cttcatattc tttatccagt cctagtctat cataatactt agtttcatcc caagcttcta
    39241 aatgaactac taaatctatc attttagaat ttttgattgc ccctactcca tataaacttc
    39301 taacatctat tattcctatt cctcttatct caagaaaatg tcttattagt tcaggtgact
    39361 gacccacaag catactttca tctaattttc ttatttctac agcatcatct gcaactaatc
    39421 tattacccct ttgaatcagt tctaatgctg tttccgactt tcctatacta ctttcacctt
    39481 ttatcagtac acctatacca taaatatcta ctagcacacc gtgaattgtt gtatgtggtg
    39541 ctaacttatt atccaaatag tttgacagtc tatttataag tctagtggta tttctatttg
    39601 tgctcaaaag tattctttta tattttttag ccttttcaat tacatctggc ttgatttcca
    39661 agtctctaga aattattaac gctggtattt catatgagaa aaatctatct agtacttctt
    39721 cccgaatatg actatccatt aatccaaaaa aattatattc tgtctttcca actatttgta
    39781 ttctttcata tgcaaaataa tcaaaatatc ctgcaaactg taatcctggt ctatttatat
    39841 cttgagagta gacataataa tctacatcat caggcatgta tactactttt aacttcaact
    39901 cttttattat ctctcttatt gatactttat tgctagtttc cattaatttt cttcctttct
    39961 aaaaaaatta taaacattat ctgcaatgct tttattcatt ccatctactt ctaaaagttc
    40021 ttccatagta gcttttttta tagcatctac atctttaaag tgatttaata atgatttctt
    40081 tcttttttca cctattcctt gtatatcatc taagatagat tttgtcaaag ctttatttct
    40141 tagacttcta tgatatgtta ttgcataatt atgaacttct tcttgtatac tagcaataaa
    40201 tctatataaa ttagttgttt tatctagttc tatttctttt tctttgcata tcaatccttt
    40261 agttctatgc ttatcatctt tgtacattcc ccaaagaggt atatctacat cattgagttc
    40321 tagtactttt tttactgcac taacttgtcc ttttccacca tccaacaata tcaagtcagg
    40381 taaatttcca tgttttaatc ttctatctac aatttcagcc atagaatcat aatcattagg
    40441 tccaataact gtctttattt tatatctcct atattccttc ttatcttttt tagcattcgt
    40501 atacactacc atagaaccaa tcgaatctac accttgtata tttgatatat cataagcctc
    40561 tattcttatt ggcaactttt ctagatttaa tatttgcttt aattcttcca atgctccaat
    40621 acttttttca tattttcttt tattcatatc agaaaatttt tcaagatatt ctacagcatt
    40681 tttcctaacc atttcaacta agcttttctt ctctcccttt tgaggaactc ttattgttac
    40741 tttttgcccc tttttagatg acagccattc ttctaatata gaactatctt ctatctcatc
    40801 ttctataatt aattctttag gtatatactc ttgttcatta taaaattgtt ttacaaatga
    40861 acttaatata gaagctcttg gactatccat taccccttct aatataaaat gttccctacc
    40921 tacaatctta ccatttctta taaaaaatac ctggacacaa gcttcattat gagctcttgc
    40981 cattgcaacc acatcttggt ttaagtcact tacagtagca tctatttttt gtttctgtat
    41041 catctcttca aggctcttta tcttatccct atatacagca gcctcttcaa atctaaagtt
    41101 catagaactc tcattcattt tttcctttaa aagttccatc aatttttctt ctttaccaga
    41161 taaacacatt ataatctctt ctatcatttt tccatactct tctttagaaa catttcctgt
    41221 acaaggtcct aaacatttat ttatgtgtag atttaaacaa ggtcttgtct tattctttat
    41281 tgctttatct atatctattt tacagcttct tattggatac gtactactta taagctccaa
    41341 ggtatcattc acagcagtta tattggtata gggcccaaaa tactttgcct tatctttaag
    41401 aactcttcta acttttaaga ttcttggata atcttcattc gtagttactt ttatatatgg
    41461 ataagtttta tcatctctaa gcactacatt atactttggt ctgtatcttt ttattaaatt
    41521 acattcaagt attaatgcct ctaactctga atccgttata atatactcaa acttatatat
    41581 attcttaacc atagatttta ctttagatgt atggtttttt gaagattgaa aatactgcct
    41641 taccctattt tttagagata tggcttttcc cacatatatt atatggtcgt atttgtcttt
    41701 cattaaatat actccgggct cagaaggtaa tttttttaaa tgctcttgta tatcaaacat
    41761 ctcgccctcc aaaacattaa attttatact aaaattatac cctaaaatat gaattttaaa
    41821 aagttataca atttccagtg ctttaaacaa gttaaagccc atattccctt ctataattga
    41881 tttgaaaaca atgttaacca tgaagagtaa tatggacttt actttgtatt actatttaca
    41941 acttatattt gactaattta cttataacaa aacagtttat cgtatatctg ctctaaaatt
    42001 tttcacaatt tatataactt aagaaaaata tttctttaag aattgacctg tataagaacc
    42061 atcaacttta gatacttctt ttggtgttcc agttgcaact atagttccac ccttatctcc
    42121 gccttctggt cctaagtcta ttacataatc acaagtcttt ataacatcta aattatgttc
    42181 tatgactata attgtatttc ctgtatctgc tagtctttgt aatacttgga ttagcttgtc
    42241 tacatctgcc atatgaagtc cagtagtagg ttcatctaat atatacaaag tctttcctgt
    42301 tggtctttta ctaagttcag cagctaattt tatacgttgt gcttctcctc ctgataattg
    42361 agttgaaggc tgtcctaatt taatatatga aagtcctaca tccattaaag tttctaattt
    42421 tctttttata tttggtatat tttcgaaaaa ctcaacagct tcttccacat tcatatctaa
    42481 tatatcagat atagttttat ctttgtattt tacttgcaat gtttctctat tgtatctttc
    42541 acctttacaa atttcacaag gaacatatac atctggtaag aaatgcattt ctatttttat
    42601 tattccatca cccttacaag cttcacatct accacctttt acattaaaac taaatctacc
    42661 ttttttataa cctcttgctt tagcttcatt agtagatgca aaaatatctc ttataaaatc
    42721 aaatacacct gtataagtag caggatttga acgaggtgtt cttccaatag gactttggtc
    42781 tatatttata actttatcta tactttcaat tccttttatc tccttatgct taccagctct
    42841 ttgcttaagt ctattcactt tacttgcaac acctttgtaa agtatatcgt ttattaatgt
    42901 actttttcca gaacctgaaa cacctgttat gcatgtaaac ttacctaatg ggaactttac
    42961 atcaatattt ttcagattat tttctgatgc tttaactatt tctataaaac ttccatttcc
    43021 ttctctagta gtctcaggaa gcaatatttg ttttttacca cttaaatatt gacccgttat
    43081 tgagttctca ttttcaatta tttcatctaa agtaccctgt gcaactattt caccaccatg
    43141 tatacctgca cctggaccaa tgtctataac ataatcagct tctctcattg tgtcttcatc
    43201 atgctctaca acgattaagg tatttcctaa atctgttaga tgtctaagag tagttataag
    43261 cctatcatta tctctttggt gaagccctat acttggctca tcaagtacat ataaaactcc
    43321 aaccaatgca gaacctattt gagtagctaa tcttatacgt tgcgcttctc ctcctgataa
    43381 agttccagct tttcttgata aatttaaata atcaagacct acatctctta agaacatagc
    43441 tcttcctttt atctctttta taatctcatg agctataaat ctctgttttt ctgtaagatt
    43501 tatattttcc ataaagtcaa tcaattcatt tacagataag tttgtaactt ccattatatt
    43561 ttttccatca actaaaacag ataaaacttc ctttttaagt ctcatccctt tacactttgg
    43621 acatggtctc tctgccatgt attcttcaat cttatctctc atgtaatctg aattagtttc
    43681 tctatatctt ctctctaagt ttactattac accttcaaag tatgatttat aatttctagt
    43741 tcctccaaat tttgactcat aagtaaactc tacaattata ttatctttac cataaagtaa
    43801 ttcctccaca aaatcatctg gtaaatcttt aaacggtgtt gtcaaagata cattaaaatg
    43861 ctctgctaaa ctttttatca ttttactgta ataagtgtca tcattaacac ctgttgaacc
    43921 ccatgctgcc actgcacctt gttttattga taaatctttg tttggtacta ctaattctgg
    43981 gtcaacttct ttactttccc caagaccatt acaaacatca caagcaccaa agggagcatt
    44041 aaatgaaaac attcttggag ataattcttc tatgccaatt ccatgttctg ggcatgcaaa
    44101 tttagttgaa aaaagaattt cttctccatc tattatttgg gctattacaa gaccatcaga
    44161 tagcttaact gcagtttcaa ttgaatctgc tagtcttgat tctattccat ctttaattac
    44221 tattctgtcc actacaactt ctatattatg tttttgattt ttatttaaga ttatatcatc
    44281 actaacttca tagttttcac cattaactct aactcttaca aatccttctt ttttgatatt
    44341 atcaaagact tttttatgtg tacctttttg tcctctaaca attggtgaaa gaatttgtaa
    44401 tttcgttcta tctggaaaat ctagcatttt atcaactatt tcttgaatag tcatctgact
    44461 tattttttca ccacatgtag ggcaatacac atctcctact cttgcaaata atagtctcaa
    44521 ataatcatat atttctgtta ctgttccaac cgtagaacgt gggttttttg aggtagtctt
    44581 ctggtcaatt gagatagctg gtgataaacc ctctatatat tctacatttg gcttttccat
    44641 ctgacctaaa aattgtcttg cataagcaga tagactttct acatatcttc tctgtccttc
    44701 tgcatatatt gtatcaaatg ccaaagaaga cttcccagac cctgacaaac ctgtaaaaac
    44761 aataaattta tctcttggta attctaaatc tatatttttt aaattatgtt cttttgctcc
    44821 tcttattata attttatctt ccataatgtt gtttttacct ttctattttt ccaatttttc
    44881 ttctaattgt ttcactttat ctctaagttc tgctgctctt tcaaattgta agttttgagc
    44941 agcttccatc atctctgatt ttaatttatc tatattagct cttatttcgt ctttatcatc
    45001 tgtatcagat attccatata caacttcttc ctctgcaacc ttagtagctt ctatactatc
    45061 tcttatattt ttctgtatag ttttaggaat gatattgtgc tcttcattat ataaattttg
    45121 tatatctctt cttctctttg tttcatcaat agcattttgc attgagtcag ttatcctgtc
    45181 tgcatacata attactcttc cattttcatt tctagctgca cgtccaatag tctgtattaa
    45241 agctgtttcg gaacgtaaga aaccttcttt gtctgcatct aaaattgcta taagtgaaac
    45301 ttctggtata tccaatcctt ctctaagtaa gtttattcct acaaggacat caaacttccc
    45361 taatcttaaa tctcttatta tttctgttct ttctaatgta actatatctg agtgtaaata
    45421 tttaactttt atccctattt ctttaagata gttagtcaag tcctcactca tttttttagt
    45481 taaagtggtt ataagcactc tttcattttt ttctatagtt ttagttattt ctcctaccaa
    45541 atcatctatt tgattattta taggtcttac ttctacaatt gggtctaaaa ggccagttgg
    45601 tcttattatc tgttctgcta ttgtctctga gtgttgaatt tcataaggcc ctggtgtagc
    45661 acttacaaac aacatttgat ttatatttcc ttcaaattct tgaaaattta atggtctatt
    45721 gtcaagtgca gaaggtaatc taaaaccatt ctctataagt gatgtttttc tagacctgtc
    45781 tcctgcatac atacctctta cttgtggtat agtgacatgt gcctcatcta caattatcaa
    45841 ataatcatct gggaagaaat ccattaaagt atatggtctt tctccttctg accttcctgt
    45901 aatatgtcta gaatagttct ctatcccctg acaaaatcct atttccttta acatttctat
    45961 atcatactta gttctttgtt caattctttg tgcttctaat agcctatcat tttccttgaa
    46021 aaatttaatt tgttcttgaa gctcgttttc tatttctaca attgcctttt caattctttc
    46081 tggcgttgtt acatagtgag atgctgggaa tataacaaca tgatttctag taccaacaat
    46141 cttccctgtt acataatcaa tttctgttat tctatctacc tcatcaccaa aaaattctat
    46201 tcttattgct ttttcatcat tactagctgg gaatatttct agtatatctc ctctaactct
    46261 aaaggtacct cgtgtaaagt ttatgtcatt tctttcgtat tgtatctcta ttaacctttt
    46321 tattacatca tctctgtctc tttgcatacc tggtcttata gataacataa gttctttata
    46381 gtcttttggg tcaccaagtc catatataca agatacagat gatattatta tagtatctct
    46441 tctctctaaa atagatgctg tagcagaatg tcgaagttta tctatttcat catttatgct
    46501 tgcatctttt tctatataag tgtcactgtg agctacatat gcttctggtt gataataatc
    46561 atagtaactt acaaagtatt caactgcatt gtctgggaaa aattctttaa actcactgta
    46621 aagttgtgct gctagagttt tattatgagc cattattagg gtaggtttct taacttgttg
    46681 aatgatattt gccattgtga atgtttttcc tgaacccgtt acacctaata atgtcgaaaa
    46741 tttctcattt ctatttatag aatccactat tgatttaata gcttctggtt ggtcacctgt
    46801 aggtttgaag tctgatttta ttttaaaatc catagtatca ctcctttttt cgaacattag
    46861 ttcctgacat gtattattat atcatacctt tataaaattg tttaagttat tttaaatatt
    46921 ttacccattt aaattttact taaatctaaa acataaaaaa agacttctat ctttgcaata
    46981 gaagtctata attcatggaa aataaatact ttattcaact tacataataa aatcaaatgc
    47041 ttctttttca actccatcta attttaaatt aaacattttt aatatagttg ctgcatgtgc
    47101 tctcaggtct ccttcttcta taaccacacc acctttaata tttggtccaa aagctataaa
    47161 tggaggtcta ttacctttag taggtaaatg accatgtgtc gcaacagcaa acttgtaatc
    47221 ttttatatct gaatctttta taatacttcc ttcgaaatca tttccaaatg aaattccttc
    47281 aagaccttca attaccattt caaaatcacc ttcaagacca aggttagatg cttcctcttt
    47341 ggtaaaaata ttctctatga ataattcttc tttaaattct tcaagcagtt ctcttaactc
    47401 tgttaaccta tctaagttat ttactattat ttgagctgat attccagaag aatttacata
    47461 ggcatcaaaa tccttaactt ctccattttc aacttttata aatccatttg atttaagcaa
    47521 gacatttgga tttataactt tatcaactct aagatgacca tggtccccaa gtattataaa
    47581 atttgtatct ttgtatgtac ctgcatcctt tgtagcctga ataatatctc ctagccattt
    47641 atcattcatt tttaatgctt tatctacttc gtcattaaat aagccataat tgtgtcgtgt
    47701 atggtccaat gtagctaaat gtattagcat taaatctggt ttatattcct ttattatatc
    47761 tactgaacag tttactccaa ataaatccat gttgggttct aatttccaat cgatataatg
    47821 acaatgtcta ttgtaaatac cgtccattat aaccttactg gaacttcgtt caaaagtttc
    47881 tcttgggtca tcttctctac taggagccca tatttctgca atattataat ctatatttgg
    47941 atttgctccc attacaggcc ataatacact agaggtagtt agattatttt cttttgctac
    48001 atctataata gtttttgctt ttatgtcctt tgagtaccag taccagtctt tattttcttt
    48061 atttgggtca aatttttcat tatgatatat accatgtttt ttaggatata ctcctgtaac
    48121 catactagta tgacacggat atgtcagagt gggataaaca caatttatat tttttactat
    48181 ggagcaattt tctagtatag atttaaagtt tggtagattt tttacaaatt ccaagtcttt
    48241 ttcaaataaa gaatctactg ataatactat aacctttgtc atttacatta cctcttcatt
    48301 tatatattat tgaatacttt taatcaactt acaagtctat ataattattt atttgtttta
    48361 tctattactg cttgcattgc agaagttcct ttgcttacta catcttttgg atttgcattt
    48421 aaatcagtaa gccccttttt aatctcatcc attagcttat cagttgcttc cttgtatcca
    48481 tttaacattg gaagtgcaac agcatattgt aattggtcag ttgcaacctt atattgaggt
    48541 ttttctttat ataatgtttg taacttttct gagtttgttg catcttcagt tgttggtaag
    48601 tatcctgttg atgaactaaa ctgaacgatt ttatctgttt gtgtcatcca ttctataaat
    48661 tctgcacttg cttctttttc tttatctgtt ccaccttcaa gcataactag attagctcca
    48721 cctgtaggag ttgcgtattt ttgatttttt ggtaagaatg ctgttgatat ttcaaactta
    48781 ccttctgttt gttgcataag tcctgctaaa tctgctgttg atgtaaatat catacctact
    48841 ttaccattta caaaatcttg ttttgctacg tcccaggcat tataatcttc tccaggaggc
    48901 attttcatta ttccagcttt catcatatct tgccagaact taactggtgc ttgtcctgcc
    48961 tcattattaa atgctacttt tttaccatta gttatttctc ctccactttg cattaccata
    49021 gcttcataga accaaatatc tattggtgtt tcaaaaccat aagtatcttc tgatttattt
    49081 gtcattttac tagcataatc cttaagctct tcccatgatt ttggacctga agggtcaaga
    49141 cctgctttag ttgccatatc tttatttatg tataatattg gtgtacttct attaaatggc
    49201 ataccaacaa acttatcctc ataatatgaa tttgttaaaa gaccagaaaa gaatctttct
    49261 tgatatgttt tatcaaaata tttactcata tctactaaag cacctgctga tgcgaatgta
    49321 cctgtttgag ttatttctaa taatgttaca tctggttggt ctttagatgt taaggctgcc
    49381 tgtagttttg ttgcattttc atagtaatct ttctgtttta acatatttac ttgtacatcc
    49441 ttatgttcac tattaaattg gtcaaccatt ttttgagcag tttctccatt agttccacct
    49501 aaaggagccc agaaatctat cttcgtaata ttgctatcag tcttttctcc tttagatgca
    49561 tttgcatcat tagaatttga acacccaact aaagaacata ataataatgt tattgctaat
    49621 cctgttgcta attttctttt aagcttcatt ttcatcctcc aaatttagtc tttaattttt
    49681 tattttattc ctgaatacat aaaagcttct tttacttttt tattcgcaaa tatataaaca
    49741 agaattattg gtaaaattag aattatattt cctgccatta ttacattcca tgcagtaatt
    49801 ccttctgaag atttaaggag cgcaactcct attggtaaag ttcttattgc atctgtattt
    49861 gtcattacta agggccagaa ataattattc caatggtata taaaattaaa taatataaat
    49921 gttattactg ctggttttgc cataggaacc attattctaa acattatttt taagtcagaa
    49981 gcattatcta gtttagctgc ttcaataatt tcatctgaaa tctgcataaa attctgtcta
    50041 agcagaaata tcccaaatga agatgtcata aatggaatta taagtggaac ataagtattt
    50101 ataaatccaa acttactcat catcatatag actggtagaa aagttacttg tggtggaatc
    50161 ataagtccta caagcaaaag cccaaatagc attttttcac ctttaaattt aatcttagaa
    50221 aatgcatatg ctgctggaac cataactaaa aattgtacaa ttaaaacact tcctgttaca
    50281 aatacactgt ttagcaaata tctaaaaaat ggacctgaat gccatgctgt actataattt
    50341 tctaacacta tatcatttgg tataatagtg ggcggtaatt gcatcatttc ttgtggattt
    50401 ttaaaggatg tagatagcat ccatataaaa gggaatataa atattgcaga taaaagtaat
    50461 agaattaata tttctattga ttttgatgct atttttttta tatcaacttt gtcttttttg
    50521 ataggttgta taatatccat tgtctcctcc ttttggataa accttataat atctactttt
    50581 tctttattga taatgaactt tctttgataa gagtctaaag ttagcatatg tagcaatcat
    50641 aactatagct aacaaaaaca tactcgctgc actcgcttgc cctatgtcat agtatataaa
    50701 tccagactca tatatataat aagataacat gttagttgaa cccactggcc caccttgtgt
    50761 cattatactt atctcatcaa atacttgaaa agatgcagtt gttgttgtta tcataagaaa
    50821 aaatattgtt ggtgataata gtggcaatgt cactttaaag aaattcgtta ttttgtttgc
    50881 attatctaat ttagatgctt catatatata gtttggtatg gatttaagac ctgataacac
    50941 tattaagata ttatatccaa gagtcttcca tattcctatt agaattagtg acattaaagc
    51001 tgaactttca cttgaaagcc actttgatgt tggtaaatgt aatgtcgata gtatccagtt
    51061 taaaaagcca tattgtggct ccataatcca aagccacaat acagctacag atactagtga
    51121 aattacatgt ggtgtaaaga ttatactttg cgttaaattg tgtattatag aatttttatt
    51181 tagccaaaca gcaagtacta tggctagtga tatagatata gtcaccataa aaaaagtata
    51241 aattgcagtg tttgttatag tttgtataaa ctctggattc tttaaaagtt ctgtataatt
    51301 gtttaagcct acattttcaa aggttgaact aagaagacta ccagatttga agcttagaga
    51361 tatcgtctta attattggat agactataaa caataaagtt cctataatag caggtgttat
    51421 atataaatat gctttattaa attgcttttt cataaatatc tcctttgatt ttagaataca
    51481 tacttttaca tacttttaca tcttctattc tttctccatt tttatcaaaa taatataaat
    51541 ctttttctaa tacacttatt cttaaagttc ttccaatatc aaatatatca ttttccgttt
    51601 tgactcttat ctctctatta agaacagata aacaatatat agtttcattt cccaacactt
    51661 ctttagttac aacttttcca aatagactaa atacatcatc atttttccca tctatgttta
    51721 tactctctag tgttgctttt tctggtctga atccaaattt aatatcattt tttatatcta
    51781 gtatgttcat acaaggactc cctataaatt gagctacaaa taaattttta ggattttgat
    51841 atatcttctt tggagaacct atttgcatta tctttccttt gttcattata actatcttat
    51901 ctgccattga cattgcctca acctggtcat gagttacata aacaaatgta gactttagtt
    51961 tttgatgtaa ttgtattaat tctgttctca tttgatttct taatttagca tctaaatttg
    52021 acaatggttc atccattaaa aatacctttg gatttttaac catagctcta gcaagtgcca
    52081 ctctttgtct ttgacctcca gataagttgc ctggtttcac atttaaatat tctctcaatc
    52141 ctactacatc aataacttct gttataagtt tttctctttt ttctttttct acttttttgt
    52201 ttttaagacc aaattctatg ttttctctaa cagtcatatg tggatataat gcataatttt
    52261 gaaataccat agctatatcc cttttactag catccgtttt tgtaacatca ttttcttcaa
    52321 tcttcaacct acctgaagtt atatcttcaa gacctgcaat cattcttaac gtagtagatt
    52381 taccacatcc acttgggcct accaatactg taaaagaacc atcttctata gtaagattta
    52441 aattttctat aactgtattt tttttattat aagattttgt tacattttct agtcttatat
    52501 ctgccatttt tatcctccca aaatatttaa tttaattcta cgatttactt atagattaat
    52561 aatactctta atttgtaaag gatatattaa gacaaagtga atatatggta aacttattaa
    52621 aataaaggtt aaattataaa cattttttgt taaatatact gaagttaaga tgatatacag
    52681 cttatgttag ctataatttt ctatattttt catattcatg gtttgctatc acaatgtaat
    52741 tttaatttat gaagagtcat ataaaactat ttatttttta tataatttgg tttgaaagta
    52801 aaaaagacct ctatggggga tagaggtctt taagtggggg agattgagta atttattttt
    52861 gctatattaa gttttacaat tatattatgt cccaaattta caaggtttaa acataaaaaa
    52921 taaaaatttt ttaattttta taaatttacc caaaaaa
//
LOCUS       DAMJYR010000012        51780 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.012, whole genome shotgun sequence.
ACCESSION   DAMJYR010000012 DAMJYR010000000
VERSION     DAMJYR010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 51780)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51780)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51780
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.012"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(<1..2029)
                     /locus_tag="PC471_000703"
     CDS             complement(<1..2029)
                     /locus_tag="PC471_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HDF2787844.1"
                     /translation="MKAGEIEFLSYLEGSNKSFVVPVYQRNYNWKKEQCKRLFDDLED
                     IIDSGFRTHFLGSVVSIYSMGKEYLIIDGQQRVTTVSILLIAMCQIIKEFGESKGIIT
                     TEEQIKECYLINKHSRDKDGKIKLKPIAEDRKAYYELFEEEMNTDRNSNIVSNYLYFY
                     NRIKEDNISIDDLFDAIQKLTIVEIELKNGEDDPQLIFESLNSTGLDLSEADRVRNFV
                     LMGRSSKLQEEFYNNYWYKIEKNTSSNVSSFIRDYLTIKERSIPNKNKVYFSFKRYVS
                     DNGIDIEILLKDLLKFSNYYREISYSCTDDNDVNKVLKRINKLEIFVSYPFILELFDD
                     YNEGIINKKVLVESLNLVECFILRRIVCDVPPNALNKVFMNLGREIKKFSDYKENYSN
                     ILRYIFINKKSSQRFPTDSEFSNAFETKDIYNTKSKNKLYILERLENYSNKEIVDLEN
                     LINNNMLNIEHIMPQTLTNKWKESLGDNYKEIHDKYLHTIGNLTLTGYNSKLSNKTFE
                     EKKEMEDGFKDSRLYLNKYISSIDKWNEEEIKNRTKILLDRAVEIWKYPSVTYEPPSN
                     SENIFTLEDDDVNFTNESIVSFSILGESYKAKNWTEFYEQVALTLYDLDSVRFNSIVD
                     KTYKRDFIDNIKSRDAYKLRKGIKISENIYLETNLNTEAKLNIIRFLLNEFD"
     gene            complement(2410..5745)
                     /locus_tag="PC471_000704"
     CDS             complement(2410..5745)
                     /locus_tag="PC471_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HDF2787845.1"
                     /translation="MGNFDFLQKKKNFNSFNTACLEAEKSILVSPSTCATITRRALEL
                     AVKWLYANDSDLVLPYQDNLSTLIHNNSFIELIDYDMLPLLKYIVKLGNLSVHTSANI
                     ERGEAILSLNNLHQFVSWIDYCYSDEYTAEDFNEDLLLHGEEKRTRPDELKNLYERLS
                     SKDKKLEEVIKENEELRKSLTEKRKVNTVNYDFKIDEVSEFETRKRYINIELKLAGWE
                     FGKDIGEEIEVQGMPNESGVGYVDYVLYGENGKPLAVVEAKRTSKDPKIGQQQAKLYA
                     DCLEKRYSQRPVIFLTNGFDMYIWDDYSDRKVYGFYKKSELQLMMDRRKSKKSLSSVT
                     INDEISNRYYQKEAIRAVCEALENKQRKTLLVCATGTGKTRIAISIVDVLSRHNWIKN
                     ILFLADRKALVKQAKKSFTKLLPNLALCNLLDSKDSPEDARMIFSTYPTMMNAIDDTK
                     SKDGKRLFTPGHFDLIIIDESHRSIYKKYKSIFDYFDSYLMGLTATPKDEIDKNTYSV
                     FDMENGVPTYAYEYNKAVEDGYLVDYTSIEFKTKIMEDGIKYDELSDEEKEEYENTFN
                     DDESIGDEIGNNAVNEWLFNSDTIDLVLNKLMTEGLKIEGEEKIGKTIIFAKNTKHAR
                     AIVERFNKLYPKYGGNFVKAVDYSINYVDSIIDDFSDKNKLPQIAVSVDMLDTGIDIP
                     EILNLVFFKKVRSKTKFWQMIGRGTRLCPDLLGVDMDKERFLIFDFCNNFEFFKFNPK
                     GFEGNKAETLTEKLFNIKVSMVKELQDIKYIEGEYAELRKELLEELITSVKALNEDSY
                     IVRMNLSYVHKYKNENVWSNIGAVAQNEIREYISPLITSYSDDELAKRFDVVMYNIQL
                     AYLQNNNASKGIRHVMATAEKLSKLGTIPQIQQQKYTIEKAMTENFWEDSDIFEVEEV
                     RISLRELIKYLEKSSQKIYYTSFEDMIVAEDRNDSVYNANNLKNYKKKVEYYLNSHKD
                     ELAIFKLRNNKKITKQDVETLEEILLKQLGNCDDYKKEFGDTPVSQLVRRLVGLDREA
                     ANEAFSEFLNNKSFNTKQIHFVKLIVDYVVKNGFIEDNKVLMEDPFRTVGSIIDLFEN
                     HIEERNKLIKTINKIKENASEIG"
     gene            complement(5795..6223)
                     /locus_tag="PC471_000705"
     CDS             complement(5795..6223)
                     /locus_tag="PC471_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2787846.1"
                     /translation="MVIRNKLSWNSIEEVNRDFGSCLYDLQNFKILYDKEEMPELWAR
                     YIKEGFKSYMVSFLELTKAMLYYKSIDLNIKSKNFYDYLLACEYHNLLPKNSSIVIET
                     LRKLRNDDSHGYDIPQFEDMYELFTENEEVFVSIRNSCKK"
     gene            complement(6213..6593)
                     /locus_tag="PC471_000706"
     CDS             complement(6213..6593)
                     /locus_tag="PC471_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HDF2787847.1"
                     /translation="MRNIPNEIKNSIINVFPKENTLAIIIFGSYGTDKHTSESDIDIA
                     WIPIKKVPITELATKTEALRNILDIDVDLKIVTDNYTIALKKAILEGEVIYESKDFKD
                     YINKFYFENSDVIDILDWRDMYGN"
     gene            complement(6626..7798)
                     /locus_tag="PC471_000707"
     CDS             complement(6626..7798)
                     /locus_tag="PC471_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HDF2787848.1"
                     /translation="MEYIKLNELCYINIGKTPSRNTSDYWGSGNRWLSISDLKEKYIS
                     KSKEEITDLAVEKANMKLVPKNTVVMSFKLSIGRVAILKEDMFTNEAIANFQIKNNEL
                     ITSEYLYYALRTLNFNNTDRAVMGATLNKSKLNDIKIPYFTICIQNKMVEVLNKAQEL
                     INKRKEQIEALDELVKSRFIEMFGEPINNTKKFDMKKIKEISIYLKRGVSPKYVEQSN
                     IKVINQKCIYWRNLKVENCKYYDENLKEKIEDIFLKQNDILINSTGTGTLGRCVKFNK
                     IDNSEYIADSHVTVLRSISNNLNSDYFAALFEFTNIQDTIYRKCVNGSTNQIELSVSK
                     LGEYLIMVPPIEFQNQFSEFVKQVDKLKLKIETNLKELEDNFNSLMQKAFKGELFN"
     gene            complement(7802..9265)
                     /locus_tag="PC471_000708"
     CDS             complement(7802..9265)
                     /locus_tag="PC471_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HDF2787849.1"
                     /translation="MITGELRSKVDKIWETFWTGGLTNPLEVIEQFTYLLFIKGLDEV
                     ETKNEAEATLLGVSFERIFDDEHQHLRWSKFSNEGNSEKMYEIVQNEVFPFIKKLHGN
                     KDSAYAKYMGDAIFKIPTPLMLSKIVDGISNLELSKDRDTKGDLYEYLLSKVATAGTN
                     GQFRTPRHIIDMIVRLIKPTPEDIIVDPAAGSAGFLVSSQQYLRDNHSSLFLVQGLKE
                     HFNNGMFYGFDMDRTMLRIGAMNMMLHGVDNPNIEYKDSLSEVNTDKDKFTLVLANPP
                     FKGSLDYEAVSADLLKVTKTKKTELLFLALFLRVLKIGGRCACIVPDGVLFGSTGGHK
                     SIRKEIVDNHKLEAIISMPSGVFKPYAGVSTAIMIFTKTGTGGTDKVWFYDMKADGYS
                     LDDKRNEIKDSDIEDIIKRFDNLDGEVDRKRTEQSFFVDVEEIRENGYDLSINKYKEV
                     VYEEVVYDAPSVIIGRVRELEKEIAVGLEELERMLEV"
     gene            complement(9775..10644)
                     /gene="gatY"
                     /locus_tag="PC471_000709"
     CDS             complement(9775..10644)
                     /gene="gatY"
                     /locus_tag="PC471_000709"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HDF2787850.1"
                     /translation="MALVTTKQILLDAQEGHYAVGAFNVENMEMVMAVIEAAEELKSP
                     VILQTTPSTIKYAGLDYYLANVKVASEKASVPVAMHLDHGSSFGLAMQALRVGYTSIM
                     IDGSHESYEDNISISKSVVDACMPSDIPVEAELGKVGGKEDDLDGGDGGAYTDPLEAK
                     EFVERTGVSSLAVAIGTAHGLYKGEPKLDLDRLSEIRDVVSVPLVLHGGSGIPDEVIK
                     ESIKRGICKVNYATELRIAYSNGVKDVLNSDPEVIDPKKYGKKGLESVKDFVKSRMEV
                     CGCVGKAIEIVKC"
     gene            complement(10664..12580)
                     /locus_tag="PC471_000710"
     CDS             complement(10664..12580)
                     /locus_tag="PC471_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIABC"
                     /protein_id="HDF2787851.1"
                     /translation="MGKKMILGVTGCPTGIAHTFMAEEALKNSARELGCDIKVETNGA
                     IGVENKLTAKDIEMADAIIVACDKNVDMDRFNGKPVIEVPVKEGIHKANELIQRCIDG
                     KVAIRKGNLSSSKIAEEGNLSFGQKLYKDLMNGVSHMLPLVVAGGVLTAISFLWGIYS
                     FDPNSEQYNQIAATLKSVGGYSMNLMVPVLAAFIAQSISGRAGMLAGLVGGMISFDTG
                     SGFLGGIISGFLAGYLVKLFVHLLRKLPRQLEGLKSIFIIPIVSVGLVGVAMLLLGGP
                     CSALNNAMMNFLSGLQNSSPIILGIVIGCMSAFDMGGPVNKAAYVTGTMLLGQGNYLF
                     MAGVSAACITPPLVIAIASTLFKNRFTEEDRAAGLINYILGSTHITEGAIPFAAKNPL
                     KVLPVLMIASSISSILTYIMKIEVPAPHGGFLVLGLVNKPLLWVGCIFVGSLVGAVLY
                     MMVTPKVVNEKNTIENTNVTKDEHKNESAESENVSNNISLYSEETVILDVKGKNKTDV
                     IDEMIGILDKSSVLLDKKKFKEEILKREEISSTGFGMGIAIPHAKTDAVKVPRVAVGV
                     SKEGFDFESEDGSLAHIIFMIAATDNGDNLHLKTLSQLSSKLMNEEFLNELLNSKTSR
                     EIVAKLNNEEIKSV"
     gene            complement(12583..13503)
                     /locus_tag="PC471_000711"
     CDS             complement(12583..13503)
                     /locus_tag="PC471_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase family hexose kinase"
                     /protein_id="HDF2787852.1"
                     /translation="MIYTLTTNPAIDMNISTNGIKRKLVNRTSNAVYSPNGKGLNVTF
                     VLGHYGIESKILGFFGGFSGKYIVEESEKRGFDVLPTWVEDTTRINIFLNDGSDEFKF
                     VNSGSYVNEKQKLDMLDKIQSLADISYLSISGSLPPGIDDNYYEDIFKICKNKSIKTI
                     LDISSPKLKELLQYNPYLIKPNDEEIKDIFGIIVRDEEDIKDVLKLLHLKGAQNILLT
                     LGEKGSYFYNGKSIYYASAQPVTVVSSACAGDSALAAFLSIWLENPEHIEEALKRSAA
                     TGASVAESNGIGNLENVEDYIKNIRVRKVV"
     gene            complement(13605..14357)
                     /locus_tag="PC471_000712"
     CDS             complement(13605..14357)
                     /locus_tag="PC471_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDF2787853.1"
                     /translation="MIIELNKSNSENLTKTELEIIKFINENESILPELSIVEIAHETY
                     SSPATVSRAIRKCGLNGFNELRYRLTVKDENDDIQNMGDIINKSFIEAQRVIEQISLT
                     TLINIIKTINKSSKIYILARGLTEYVAKEFSLKLQLLDFNVVFINDPNIMKIKSKSIK
                     KDELIIIFSLNGNTIELVESAQNANLVGAKVITCCCSESAKLIKYSDLYIVGYKHEHI
                     SISEYEVSSRLPLYIISRIIIDYMVRYNNSEN"
     gene            complement(14749..16194)
                     /locus_tag="PC471_000713"
     CDS             complement(14749..16194)
                     /locus_tag="PC471_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HDF2787854.1"
                     /translation="MIKLGKFPKGFLWGGAVAANQLEGAWNEGGKGWCLADVHMYDKD
                     RDISKPYESDMTIERIKFAMKDNNGYYPKRSGIDFYHTYKEDLKLLKELGLKCFRFSI
                     NWARIFPNGDDLEPNEEGLKFYDNLINEVIDNGMEPIVTLLHYETPLNLVLKYKGWSN
                     RKVVDFFVRYAELILNRYKNKVKYWIVINQINLIHYESFNSVAFCVDQVDNVEETRYQ
                     AIHHQFIATALTKKLGKEINSDMMIGMMMADCTSYPEDCDPENVVFALKRNRLQYFFT
                     DVSFRGEYPVYIKRFFKENNINIVMEEGDDKILKENTMDFLALSYYYSTTVSASKNTM
                     NPTDNIKNPYLKANPWGWAIDPKGLYNTLSQYYDRYQKPIMIAENGFGMHDKFEDGKV
                     HDSYRIDYLGKHIEQIGEAIADGTEILAYCMWSPIDIVSCSSQEMEKRYGFIYVDIDN
                     NGNGSKERYKKDSFYWYQNVIRTNGEELREK"
     gene            complement(16216..16569)
                     /locus_tag="PC471_000714"
     CDS             complement(16216..16569)
                     /locus_tag="PC471_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HDF2787855.1"
                     /translation="MTIEEHIIDEELVEVAMQIILRAGEARTEIKHALNDLEKFDYRN
                     ADLKLAKAKGFMTEAHRAQTKIIQGEAGGEKRAHSLLFAHAQDTLMTIFSELNITMSL
                     VGIVKSIEYKVNNSK"
     gene            complement(16598..17845)
                     /locus_tag="PC471_000715"
     CDS             complement(16598..17845)
                     /locus_tag="PC471_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDF2787856.1"
                     /translation="MGRVFMNKFIDFLSEKFTPVVNNMTKNIWVQSVQSTIMKVLPMV
                     FVGSLVTIVSVLKNYISFLPDLSPINQYTFGLLGLFIAFLLPMETMKNKKFESMSVVA
                     GLAGAGLMLMMIRPEITNEGAIFNFNRFGGEGMLVSLVAGLFSGLIMSLFGSFSFFGE
                     DSALPDFVSKWFDNMLPITVIMFVGWGLIYGLNFDMFEIIVRVFKPVSDIAQTLPGFV
                     LLNFLVVFIYSLGISGWVLSPITYSIQLGAIAANAAAVASGGVATNIYVYEVIQTGWI
                     TLGGLGATLPLVIMMCLSRVGRIKAIGRACIVPSIMNINEPVIFGAPVAWNPFLMLPM
                     WIGTVVISITTYLSMSMGFVTIPSKVFNLWYVPFGISTFMVNQDVRGLIVLAINIVLL
                     FMIWYPFFKAYEKEEIKLESQEV"
     gene            complement(17853..18170)
                     /locus_tag="PC471_000716"
     CDS             complement(17853..18170)
                     /locus_tag="PC471_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDF2787857.1"
                     /translation="MKNIRVLLACGSGASTGFMATSMRKSAKKKGIEADIKARSESEI
                     DKYLSEIDCLLLGPHLDYMKEEIQQKANEYNVPVACISKTAYGRLDGAKALEEALELI
                     SNK"
     gene            18412..20325
                     /locus_tag="PC471_000717"
     CDS             18412..20325
                     /locus_tag="PC471_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HDF2787858.1"
                     /translation="MKTSKKELFLNFIKTQHDWIDSSTLANYLNVSTRSIRKYVNEIN
                     SNGAFILSSKKGYKVNLNTNCQTKIDSSENISPDNRLNLILKELIVNSNGINIFDLSE
                     ELFVSPATIEGDIVKANKFIGSYNLKIKQSKFLLKLVGNESAKRKLMSSIIFKETGSD
                     FLSLFDVQKIYQEYNLTKLKENIIYILKKYNLFINEYAINNILLHLMITIDRIKKNNY
                     IDSVEVVNYIDNNVDINIAKDISNFLESEYNITLTSAELYYLVFQLTNKTTVLNYNQM
                     DTKSLSNYIDEHFVKLTKKIIKNVYDLYLIDLSDEEFVVKFTLHVKNLISRAKNNQVL
                     RNQIPQKLKDSYPLIYDISVYICNQIQTLENVDIDEDEISYISLHVGSFFDRQKLLED
                     KVLCALITPNYYDLQFKIVRDLEKRFNESIEIIQIFSDTHNLDFDNKVDMVITTLPIN
                     DRCPIPFVYVNPYLNRKDYDNIQSKFIQIKDRKNILTVQNHLEMYFSESLFMKNIYLD
                     SAKDYIRFMGNILYENKYVKPNYIDDVLIREKMSSTAFNNNVAIPHSMKMDALKTGVC
                     LIVNDKPVKWGEEKVQIIAMIPINEKEKEKFNYIFESFIEILSEWNNVKELTKADNYS
                     SFMNRIAYLIQNI"
     gene            complement(20448..21602)
                     /locus_tag="PC471_000718"
     CDS             complement(20448..21602)
                     /locus_tag="PC471_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HDF2787859.1"
                     /translation="MKILISIDSLKGSLSSIEAGNAIKKGILKVKEDAQVKILPLADG
                     GEGTVDALVQGMNGKKETIEVTGPIAKKVDATYGLLKNTSTAIIEIAQASGLTLVPTE
                     LRNPLYTTTYGVGEIIKEAINKGYRNFIVGIGGSATNDAGIGMLQALGFEFYDKNNKL
                     VGLGGKVLNEIRHIKIENRLKELDECKFKIACDVNNPLFGKNGAAYIYGSQKGATSEI
                     IEKLDNGLRNFSKVVKNYLSKDVANVEGAGAAGGLGFAFLAFLDSKLESGIKIILEEI
                     KLEEELKDADFVITGEGRLDNQTAMGKAPIGVAKLAKKYGVKVIGLAGATTEDAVKCN
                     EEGIDAYFSIVNRAMTIEEAMDKATASENMTATTTQIFNLITSIQQSKNM"
     gene            complement(21881..24496)
                     /gene="mngB"
                     /locus_tag="PC471_000719"
     CDS             complement(21881..24496)
                     /gene="mngB"
                     /locus_tag="PC471_000719"
                     /EC_number="3.2.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosylglycerate hydrolase"
                     /protein_id="HDF2787860.1"
                     /translation="MKKNVHIVPHMHWDREWYFSTEESRILLVNNMEEIMDMLENNHN
                     YPYYVMDGQTAILEDYLAVKPECKERIKKLVQEGRLIIGPWYTQTDEMVVGGESILRN
                     LLYGIKDCDEFGEYMKIGYLPDSFGQSAQMPQILNGFDIKYSMFWRGCSERKGTNKTE
                     FNWKSDDGSSVLVQILPLGYAIGKYLPMNEDELRTRMDKYLPVLDKGATTDHIILPNG
                     HDQMPIQKNIFEVIYKLKECYPDRKFFLSRYENIFKELEKNEDIDTIKGEFLDGKYMR
                     VHRSIFSTRMDIKSANARIESKITNILEPLASIAYSLGFEYHHGLIELIWKEIMKNHA
                     HDSIGCCCSDKVHQEIMNRFFLAEEKVDQLITFYKRRIVDAMSCEMALDKLIAFNLMP
                     YDRNEIVSAQVITKMKAFEIFDKDNNKLDFEVVHKEVIDAGLIDRQIVHYGNYDSFMV
                     YTINFKDKIPAMGYKAYMIKEVEHMLEKAYEVCDMVDNDFYTIQVNENGTLKIFDKKL
                     NKTFENVLLMENGGDEGDEYDFSPLPNEKLIFNDNIVANSTIKKNRFNNEIKINYRLK
                     VPKDIQARKNNRADSFIDFDIVINVPNDKAIIDVVFNINNQARDCRVRTYIPTNIASK
                     FSVSDNQFGHIKRSVYDDAMEVWQQEDWSERPDSIYPMLTFVGLSDKEHGVAVLTNST
                     REFEIVGEKFDTIAITLFRSVGFLGKEEMARRPGRPSGIKLPTPDSQMIGDIKIDLAI
                     VTHEKSTLEANVANMAKEYLTKIQTYNKMPYNAMKLNQSEIEVDYSYSLLKETNKDLV
                     LSVVKKAEKEEALVVRFYNSKEETKSANIVFNKNIKTAKWINLNEKELESISIDNNSV
                     NVECLMNQVKTILVK"
     gene            complement(24640..26565)
                     /gene="mngA"
                     /locus_tag="PC471_000720"
     CDS             complement(24640..26565)
                     /gene="mngA"
                     /locus_tag="PC471_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS 2-O-a-mannosyl-D-glycerate transporter
                     subunit IIABC"
                     /protein_id="HDF2787861.1"
                     /translation="MEFKKITNENLVFLKQKIKTKEDAIKFLARKLYDEGKLTSYEEY
                     LEAVMYRETLSPTGFERGLAIPHGKSKSVKEAAFAAMTLETPIENWESVDPNNKVELI
                     FLIAVPESEGGNTHIQILSELVTRLSNEDYMNMLLNSKTKKELFNNLDIKEKKEQIKE
                     EIRIQNESKKIILAITACPAGIAHTYMSAEALIKAGKEIGVDVYVEKQGANGMVDPHT
                     PDIIKRADAIIYATDVAPKNTERFEHLPNIKTSVAAPLKRAKEIIYEALEVAQKQGKG
                     DYIEKSSDISYCEKSSWKKDVKSAILTGVSHIIPLIVAGGMILAFAVLASQAFGLQEL
                     YNQEGSWLWQLRKLSGGMLGTLMVPVLSAYMSYSLAEKPGLGPGFAAGLAADLIGSGF
                     LGGMLGGLLAGYIVKFLKKNIPAKGTLAGFVSFWVYPVVGSLVVGLLMLFVVGKPVAA
                     LNNGLINWLNGMTGVNAILLGAIIGAMVSFDLGGPINKAAYTFAIGAIASGNFIPYAI
                     FASVKMVSAFGVTIATVLGRDLFSESEKEIGKQTWILGLAGITEGAIPFMIEDPIRVI
                     PSLVAGSAITGSIVAFFNIGLQVPGAGIFSLALLEGSMSGFIIASIWFFAAVLGAIIS
                     AILLIITRKQKNQRKIH"
     gene            complement(26695..27420)
                     /locus_tag="PC471_000721"
     CDS             complement(26695..27420)
                     /locus_tag="PC471_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDF2787862.1"
                     /translation="MEFYERAKKYEYKFTDTEDMIIEYMRKNKEEIVKLSIQSLADKF
                     FTVPNTIVRLCKKMEYDGFSQLKNNLKEELKNEDKEPKNGFESDIKKTLELIDYEKIN
                     KVADIIQKSRSIIVYGIGQNIPLCEILVKEIRGGHDNITYYSQRHEVLHDIGKLGEQD
                     MFFALSLSGETKEVIEAAKTAVNKGCKVVALTHICENQLQQIADISLYCYSTIKQVNG
                     YNISDKVPIMMIMRAIIEQYWLR"
     gene            complement(27683..28462)
                     /locus_tag="PC471_000722"
     CDS             complement(27683..28462)
                     /locus_tag="PC471_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChbG/HpnK family deacetylase"
                     /protein_id="HDF2787863.1"
                     /translation="MHKMKKLVVRADDIGYSEGVTLGIISSYKNGIVTSAGIMVNMPF
                     SRQAIEMVKNDDVCFGLHVNLVIGEPCCKEHIKIKNLLDEGGKFVSSKIRRTQLLTGK
                     EVFNYEEVYEEVCAQIETYKLYMSKLPEYIDIHGIEEDVSISAMCDAAKEYGIPSCPY
                     YLDSCISMPSKYPQNDFYKLNKSFVKLFEEKYINLDKDITLMVTHPGFIDYSLIKSSS
                     LIQERVYEHAMLCDKEVKMWLKRNNIELVDFKDIFKSNNYI"
     gene            complement(28455..29882)
                     /locus_tag="PC471_000723"
     CDS             complement(28455..29882)
                     /locus_tag="PC471_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDF2787864.1"
                     /translation="MMQQLSLLGKTMLVPIAVMPAAGILGLLFGPNMLNVPAISNISN
                     IVFSNVDYLFLLGAASAYSKSKDKTSVMVAAVVAYMIFKSNLELLNDTLNAGVFGGII
                     VGACIAWTYNKTYKVKVPAFLSFFAGEKCVITLSPVVALILSYIFSLLWIYPQAAMNQ
                     FGFWLGGAGAIGIFLFLFCNRALIPTGLHQVLNAYILFEMGEYTTSTGEVIRGEIPRF
                     MAGDPNAGFFWTGFYIIMMFSIPAISYAIYKTAQDKKKQEVKGAMTAGALTSFMATVT
                     EPIEFSFLFASPKLYIIHSFYSGLGGVVLYLLGARLGSFNGASIIDYVLSFSYGDKAW
                     LVIPVGVVFFVLYYVTFKYIIIKDNVQTPGREVEIEIGDKVSEKEKNLKLSHGNFSYM
                     AKQIIKNCGGYENIVTLNNCMTRLRLEVKDATILNDDNIKKTGAKGVIKLSNTSVQII
                     IGTDVVKVKDEMEMQLDELRKQADA"
     gene            30273..30995
                     /locus_tag="PC471_000724"
     CDS             30273..30995
                     /locus_tag="PC471_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDF2787865.1"
                     /translation="MAKNKFSEIYEVLKEEILDGKYTSNMMLPTELQLIERFSCSRNT
                     VRRAISQLNTEGYVQSIKGKGVVVLENSCSNDFFLNMHNFKGVESIVEDKKVNTATSV
                     LHFSKILIDNKLSKKTGFKVGSEVYYLHRLRYIDNIPKILDINYFLCSIVKDLDVSIA
                     QGSIYKYIEECLGTKIVSSRKIFKIEKATELELKTLPLNDYNCVGVIKNSVYTDDGKL
                     FEYTESKHTPETFVFMDVTQRY"
     gene            31192..32859
                     /locus_tag="PC471_000725"
     CDS             31192..32859
                     /locus_tag="PC471_000725"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HDF2787866.1"
                     /translation="MKNWWKKATVYQIYPKSFKDSNNDGIGDINGIIEKLDYLYSLGV
                     DLLWLTPMYVSPQRDNGYDIEDYYNIDPKYGTMSDFEKLLKEAHKRDIKIMMDMVLNH
                     TSTEHKWFKESKKSKDNPYRDYYFWKDAKPDGSVPNNWISRFSGTAWKYDETTNQYYL
                     HLFEETQADLNWENEKVREECYKILEFWADKGIDGFRLDVVNLLSKTPGLPDDPITGP
                     KGDGRTHYADGPRIHEYLHNMNQKVFKPKNIVTVGEMSSTTPEECINYTRENREELSM
                     VFSFHHMKTDYKGGAKWTNEMFSLDKLKKAQSDFQYKMYEGKGWNALFYSNHDQPRAL
                     SRFGDDRFFRQESAKMLAITLFGLQGTTYIYQGEEIGMTNAYFTKIDEYDDAESKNAY
                     YHMIKSGIDEKEALLILQQKSRDNARTPMQWDNTIYKGFSNHKPWLKVNNDNISVENE
                     LNDSRSVFYTYQRLIKYRKQYDIFTEGTYRLLDENHKNLFVYEREYKNQNLLVISNFT
                     HDNVVYKLPETYLNKLNYTILETNYTRDIIENKMEIKPYESIMIYYK"
     gene            complement(32955..34274)
                     /locus_tag="PC471_000726"
     CDS             complement(32955..34274)
                     /locus_tag="PC471_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDF2787867.1"
                     /translation="MEKGIKLGFWSIVLLGINAIIGSGIFGLPGDAYTDIGPASILVL
                     VFCMLLAVSIALCFAEAGSWFDTDGGPYLYAKEAFGDFVGFEVGFMKWIVSMIAWATM
                     ANFFAVTLSSVWPQAAEPLTKNIIIGILVVGLGIINFMGMKQSKHLNNIMTIGKLLPI
                     VLFIAVGLFFIKGSNFTPFVIIQKGQSASSAFVAVSITLFYAFTGFESLAVAAKDMEN
                     PKKNVPKALVMVMFVVSVIYMLILGISIGVLGNGLAGSATPVADAAIKMLGPIGGYII
                     TIGTIVSVGGINIASSIFTPRSAAALVEQGLMPKSIRKTNKNGAPYIAIIVSVIGTLL
                     IAWSGSFTTLSQISVVSRFIQYIPTCLAVLILRKKYAGKDVNFRIPGGAIIPIFAVII
                     SILLLIKAGIDEPMKIVWGLGGMIIVVPIYFYMTKVYSKKYNDVEVK"
     gene            complement(34315..35061)
                     /locus_tag="PC471_000727"
     CDS             complement(34315..35061)
                     /locus_tag="PC471_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HDF2787868.1"
                     /translation="MKKLAKKKYPIIGISGNLLIDEGGMFPGYERAYVNNDYIQSVVM
                     CKAIPYIVPIVYDDEIIKEQVSNIDALILSGGQDVNPLIWKEEPHNKLGAISPKRDSF
                     DMKLLKHALDMKKPVLGICRGEQIINVTEGGSLYQDLSLIEGAYIKHNQQHLSNVPTH
                     TVQIKEGTKLYEILGEKEVLVNSFHHLVVNKVAPGYIVSATSKDGLIEAIEKEGSEFV
                     IGIQWHPEMMTRDYDNMKKIFMAIVKEASK"
     gene            complement(35305..37314)
                     /locus_tag="PC471_000728"
     CDS             complement(35305..37314)
                     /locus_tag="PC471_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDF2787869.1"
                     /translation="MKTIAVVTDGLSKLECFLNKNLEMIFKDKVMIKNYYLNKITEKE
                     LIKDDIILVMIDDRVLKIKKYVEDVSKIITINRSIKQKDIYKLFSLPEGIDVLVVNDN
                     KHTVLETISNLYNIGINHLNFIPYNPNEEYRNIKIAITPGEASLVPKYIEEVIDLGHR
                     YIDSSTFIQIIAKLKLDDKEITERLIKYTDEIISLYSGINTKYKELTIKADELNSIIN
                     LSNLGMAMVSDKGNIIICNNSLGDILDIQSNIIGKNIDELENENIKKIFSLSKAHDEV
                     IKFNNKYLNVNKYNIESFGRVTGYYFCIQEITYIRKLEQNLSKKLREKGQVARYTFDD
                     IKTVSTKMERTKKLGMKIAESDYTILITGESGTGKELMAQSIHNASPRHSQPFIAINC
                     AAMPENLLESELFGYEEGAFTGALKGGKKGLFEQANNGTIFLDEIGDMPIYLQSKLLR
                     VLQENQVMRVGGENVIDIDVRVIAATNKNLLEMIKGDRFRADLYYRINVLPINIPPLR
                     ERKEDIEVMLKHFMKRKRAISQDVQDIINAYEWPGNIRELKNTAMYINIMSSEDVILI
                     NDLPHNLLNITEDFSKEISILKDRTSIEKVKLVMECLKNANNLNKNIGRNGIVNSINE
                     LGYNITEGEVRGILNMLKELDLIICESGRKGSELNRRGKNILNSI"
     gene            complement(37752..39185)
                     /locus_tag="PC471_000729"
     CDS             complement(37752..39185)
                     /locus_tag="PC471_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HDF2787870.1"
                     /translation="MALRKDFLWGGATAANQCEGAWNLDGKGPSCSDMCTGGSQKVSK
                     RITRVIEKGTFYPSHEAIDFYHRYKEDIALFAEMGFKVFRFSIAWTRIFPTGMEKEPN
                     EAGLKFYENVIDECLKYNIEPLITISHYEIPFAITEKYNGWVSRDVIDLYMNYCETIF
                     KRYKGKVKYWLTFNEINSATMPMGGYLSQGILNEGTTDFINQVDIPQLRFQGLHHQFV
                     ASAKAVKLAHEIDSNYQVGCMQIFATMYPYTCNPDDAVKTQRDSRVMNYFCGDVQVRG
                     EYPTYMNRYFGENNIEIKMEKGDLEILKEGCVDFYTFSYYMSTCVSSDSKEDNTSGNI
                     LGGVKNPYLKSSEWGWQIDPEGLRYALNEIYDRYRIPMMVVENGLGAYDKKESNGVIN
                     DDYRIEYLKAHIEQMKEAVEDGVDLFGYTPWGCIDLVSASTGEMAKRYGFIYVDKYDD
                     GSGDLSRIKKKSFDWYKQVIESNGERL"
     gene            complement(39272..41188)
                     /locus_tag="PC471_000730"
     CDS             complement(39272..41188)
                     /locus_tag="PC471_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDF2787871.1"
                     /translation="MRKYEQLSKEIIANVGGKDNISSLSHCITRLRFKLKDESIANDD
                     VLKKMDGIVTVMKSAGQYQVVIGNHVPDVYEVVCDLAGLGGDTIDNSDSDAPKGLLNK
                     FIDIISGVFQPILGVLCAAGMIKGFNAVFTALGLYGETDGAYIMLNAIGDAMFQYMPI
                     ILGYTAAKKFKLKPFIGMLIGAVLCYSGIQLDTLASTGQPLYNLFTGTIFESPVYITF
                     LGLPVIAMNYTSTVVPVILIVLLAAKLEKVFAKIIPDVVKNFFVPMAVLLVSLPLGFM
                     VIGPIATYASNIVGNGFLALFNFSPILCGALTGLLWQVLVIFGLHWGLVPIAMSNLMT
                     MGFDTILVGAFVPSFAQTAVVAAMYFKLKDKKIKELCIPAVISGICGVTEPAIYGITL
                     PRKTPFIYSCVGGGVAGAVMGIMNVKSYVMGGLGIFGIPNYINPTTGDMKGVYATIIA
                     IIVAIVVGFVLTLFFWKDDAIVEISKDDNGQGVIEKNKREVVYSPIKGEVKALEQIQD
                     AAFSTGILGKGVAITPKEGKVVAPFDGTVMTLFPTKHAIGIISDNGCELLIHIGLNTI
                     QLEGKYFKSFVNQGDKIKKGQILITFDIESISKEGYCLETPVVVTNYSDYVDILENTQ
                     KHVNNSQELITVLA"
     gene            complement(41262..42122)
                     /locus_tag="PC471_000731"
     CDS             complement(41262..42122)
                     /locus_tag="PC471_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDF2787872.1"
                     /translation="MIIEKILNNNVIITTDENHKEIVVMGRGLAYKKRTGEHISKDKI
                     DKIFKLSDPNISDKFKELIADIPIRYMELSDEIILYAKEKLGKRLNDSIYISLTDHMY
                     TAIERAREGVSVKNVLLWDIKRFYKSEFKIGLEALDYIEKKFEIRLSEDEAGFIALHI
                     VNAQMDQSIKTIYEITQIIQEISNMVKYHYRIVFDEDSVYYHRFITHLKYFAERIVSN
                     NLHENNEDDLLNVIKVKYKNAYKFIEKLDEFIHKKYNYDLTDEEKLYLTIHVERVVSK
                     SIKSDINPNN"
     gene            complement(42473..43660)
                     /locus_tag="PC471_000732"
     CDS             complement(42473..43660)
                     /locus_tag="PC471_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HDF2787873.1"
                     /translation="MIESIKKRAYEIENELGKEIEEVCNFIFNNPELGEEEYISSKYL
                     VEKMKEYGFDTVYPYCNMETAFRAELGDNDGPTIAFLAEYDALPGYGENKEPAHACGH
                     NWIAASTLGACIVLSKLKENFKGKIVLIGTPAEETTGGKCDLVKAGAFDDVDVSYQMH
                     IEAFNNINCKALAIDSLEFSFEGVSAHAASHPHMGVNALDAVQLTFAGINALRQHVKS
                     DVRIHGIVSNGGEAPNIVPEKAACKFFVRAAERSYLDEVTKKVINCAKGAELMTGAKL
                     SYRYFENSFDNIINNKVLQKITKNNLIEAGITDILEGKDGPVGSTDIGNVSQVCPTMY
                     TEIALDISPMVYVHEKEFLNYANSEEAYDKLHKAVKAMVGCALEIYLEDGLLDEIKKN
                     HLN"
     gene            complement(43674..45065)
                     /locus_tag="PC471_000733"
     CDS             complement(43674..45065)
                     /locus_tag="PC471_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HDF2787874.1"
                     /translation="MSSLKKKKFTMPQTLTIIFLLIVLMAILTWIVPSGNFERVDIDG
                     RSVVVAGTYEKAPSNPQGITDVFTAPINGFIDAAEVVGFVLIVGGAFGIVNKTGAIEA
                     VIAHTVNKMKKFQFLIIPISMILFGLGGTTFGMSEETLPFYMIFIPLMTSMGYDSLTA
                     VATVFIGATAGFGAATTNPFSVGIAQALSQIVPGSGIEFRVVMFIIYMAISIGFVMMY
                     ANKVKKDPKKSLVHDISLNQELMVNSDTNIKEFTKREAMVIAIFTIGMAIMIYGVLRL
                     EWYITEIAMIFTAIGIISGIASGLKQDEIVNSFISGAGDLITAGLCIAFARGIVIIAE
                     NGFIIDTILNSAANLLNGLPKTIFINLTFLIEGLIAFLIPSASGLASLTIPVLAPLGD
                     LVDVSRQMIVTAYQFGIGVTNLITPTSGVLMGALAVANIPWSKWVRFVIPLMVVLTIL
                     VMAFLTIGLYLGF"
     gene            complement(45660..47360)
                     /locus_tag="PC471_000734"
     CDS             complement(45660..47360)
                     /locus_tag="PC471_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDF2787875.1"
                     /translation="MHYIAWHFNKNGGNNTMKDKLQVLSGAMMIPVILLVVAGVFIGL
                     GSAFANMENVSALGLSGLIKEGSFINVFFKVINDLGFMVMRYLPIFFVTGIAFGLSKK
                     EKGWGALGGIVLFIGMHTVISTLLAANGINSDTVTAEAFMASGLGEAEAYSRSSLYGS
                     FLGIFSYDCSIFGAIIAGFVASAVHNKFVDIELPSALSFFSGPRFSMIMMFIASIVLG
                     TAMYFIWPPVGLALSKLGNWIGSSGLLGTFTFGAMDKALLPFGIHHLIAFPIEYTRVG
                     GTMEVGGAIYEGVNNIRLAQMGDPDTLSYITRNFTTGRLLIHFAILPAAALAMYKLAD
                     NANKKKAISILIPAIVTAMLVGVTEPIEYTFLFVAPLLYFVAYVPLAGLTYVFTEMFN
                     VSIMGESFRNMFPNLLQPQKVDALPLLFLIPIFFIVTYIIFTWAIKKFDIKTPGRSSE
                     EVKLYSKKEYRERQSVDAGKTAYNNAVEEEVDDKDTKLVHSIIEGLGGSNNIKNVTNC
                     ATRLRVELNSIDGFYEDGFWVNELGASGVVKKKNSVQVIFGPRVITIASKLKAVLGVD
                     "
     gene            complement(47332..48735)
                     /locus_tag="PC471_000735"
     CDS             complement(47332..48735)
                     /locus_tag="PC471_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HDF2787876.1"
                     /translation="MIVNKELIIDYLKSNQENIINDIRSLVEIPSIRDESTTDINQPF
                     GIEIRNAFDKLIQIAKDKDFVVKDFDGYAIHIEYGEGEEVVGVLNHIDVVPIYNKELW
                     KSKPFKVCQKDNYLYGRGVNDNKGPLIGILYALLFLRELNEKPKRKIRLIVGGAEETT
                     WECMEHYFSVNEQPKFAFSPDGNFPIVNGEKGILYFNLRKKIDKDKFRNHNLVDIKSN
                     KEDGFVCDKIEAVFKTNDKKDLVESLAYYTEIEELDEGKVLVRYTGERALSRNPHRSY
                     NCAFSLAKDLEKIKKLNDKGVIIRDILNSYFTDDNHGKKLGMYKEDVDMGVSTICIMS
                     IFLEKNELNMKIDFRYPKGISWEFITNRINEIGKKENLIVDIYKDLKLLYVKPDSELI
                     NKLSNAYKQGFGKEAELFTKGAASYARVLKNGVAFGPTIEGDNPNSHQANENISIDTL
                     YKAIEVYIYALYSLAFQ"
     gene            complement(48999..49490)
                     /locus_tag="PC471_000736"
     CDS             complement(48999..49490)
                     /locus_tag="PC471_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDF2787877.1"
                     /translation="MFNIFKKKENKIEKIYSPFKGNLVNLEDVPDAVFAQKMMGDGVA
                     IIPSEGKLYSPVDGEIVNVFDTKHAIMIKSNDNTNILIHVGLETMSLEGKPFDVKVKA
                     GDKVKAKDLIMEVDLDFITSNDLNIITPVVIMDDADSVKRTVKDRIEPGNVDINSIIM
                     EVE"
     gene            complement(49516..50328)
                     /locus_tag="PC471_000737"
     CDS             complement(49516..50328)
                     /locus_tag="PC471_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDF2787878.1"
                     /translation="MRYKLICTDMDGTLMGKGFEVSEENIKALKEAMEKGIKVALVTG
                     RPYNAMKYFTSVLGDDIYIISTNGTYFKLLGYEYKKVLSKEALKKIYTIGEKYNLNKH
                     FKGCKIVISNNEIGEEHPYRLINSKNKEEDRIEIIENASCETLLEKADNEILKCILFS
                     ENVDSLREAKEEFKKQEDLEVVSSGKINFEVMSKGTSKGIAVKKFCDILGIDSKEVIC
                     IGDNENDISMIKFAGLGIAMGNATDEVKSMADFVTDTNVNDGVAKALRKILS"
     gene            complement(50341..51300)
                     /locus_tag="PC471_000738"
                     /pseudo
     CDS             complement(50341..51300)
                     /locus_tag="PC471_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422425.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
     gene            complement(51418..>51780)
                     /locus_tag="PC471_000739"
     CDS             complement(51418..>51780)
                     /locus_tag="PC471_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDF2787879.1"
                     /translation="QKLSTKLIKENDIICIEDLQVKNMIKNRKLSRSISDVSWSEFIR
                     QLEYKANWHGRQIVKVGKFFASSQICNKCGYKNEEVKDLNIREWICPSCNETHDRDIN
                     ASINILKEGLRLITIQNK"
BASE COUNT        16542 a         9521 c         5478 g        20239 t
ORIGIN      
        1 tatcaaactc attaagtaaa aatctaatta tatttaattt agcctctgta tttaaatttg
       61 tttctaaata tatattttct gatattttaa taccttttct taatttatat gcatctctag
      121 acttaatatt atctataaaa tctcttttat aagttttgtc tacaatactg ttaaacctta
      181 cagaatccaa atcatataaa gtaagagcaa cttgttcata aaattctgtc caatttttag
      241 ccttatatga ctctcctaat atactaaatg atactatact ttcatttgta aaattaacat
      301 catcatcctc taacgtaaat atattttcac tgttacttgg aggttcatat gtaacacttg
      361 gatatttcca aatttctaca gctctatcta gtaatatctt agttctattt tttatttctt
      421 cttcattcca tttatctata gatgaaatat atttatttag ataaagccta ctatctttaa
      481 atccatcttc catttctttc ttttcttcaa atgttttatt acttagtttt gaattatatc
      541 ctgtcaaagt taaatttcca attgtatgta aatatttatc atgtatctcc ttatagttat
      601 ctcctagtga ttccttccat ttatttgtta atgtctgtgg cattatatgc tctatattta
      661 gcatattatt attgattaaa ttttctaaat caacaatttc tttattacta taattctcta
      721 acctctcaag tatatataac ttattctttg acttagtatt atatatatct ttagtttcaa
      781 aagcatttga aaattcactg tcagtaggaa atctttgaga tgatttctta tttataaaaa
      841 tatatcttaa tatattagaa tagttttctt tataatcact aaatttttta atttcccttc
      901 ctaagttcat aaatacttta ttcagtgcat tcggaggtac atcacatact attcttctta
      961 atataaaaca ttctactaaa ttaagggatt ctacaagtac ttttttattt attatacctt
     1021 cattataatc atcaaataat tctaatataa acggatatga tacaaatatt tcaagtttgt
     1081 ttatcctttt caaaactttg tttacatcat tatcatcagt acatgaataa gatatctctc
     1141 tataatagtt agaaaattta agtaaatctt ttaataaaat ttctatatct ataccattat
     1201 ctgatacata ccttttaaaa ctaaaatata ctttattttt attcggtata cttctctcct
     1261 ttatagtcaa atagtctctt ataaaactac ttacgttgct acttgtattt ttttcaattt
     1321 tataccaata attattataa aattcctctt gtaacttaga gcttctaccc attaacacaa
     1381 agtttcttac tctatcagct tcacttaaat ctaagccagt tgaatttaga ctttcaaaga
     1441 ttaattgagg gtcatcttca ccatttttta actctatttc tactatagtt aatttctgta
     1501 tagcatcaaa caaatcatct attgatatat tatcttcttt tattctatta taaaaatata
     1561 aataatttga aactatatta gaatttctat ctgtattcat ttcttcttca aatagttcat
     1621 agtacgcttt tctatcttct gctattggtt ttaactttat ttttccatct ttatctcttg
     1681 agtgcttgtt tataagataa cactccttaa tctgttcttc tgttgtaata attccttttg
     1741 attccccaaa ctcttttatt atttgacaca tggcaatcaa taatattgac acagtagtca
     1801 ctctttgttg tccatctatt attaaatatt cttttcccat actatatata gatacaactg
     1861 accctaagaa atgagttcta aatccagaat ctattatatc ctctaaatca tcaaacaatc
     1921 ttttacattg ttctttcttc cagttataat ttctttgata tacaggtact acaaaacttt
     1981 tgtttgaccc ctctaaataa ctaagaaatt caatttctcc tgctttcata ttaactcccc
     2041 ctataagtta aataaattcc attcataact ccatgtatct atatattata tcaaaatttt
     2101 gactcataaa tgtatatgaa agccttatta tatacctttt tatacctgtt tttattttat
     2161 taaagtaaat acatgattat tctataaaat atacaaatac ttaattttaa aaattattat
     2221 ttaatcttct tcatgagtaa ttcatagtta tttatcaatt aaaaagagat attgcataat
     2281 tattcaatac ctctctttgt atatttacat tatccatatc tagttatcta tttcaaccca
     2341 atattagact acctactaat aaatttattc tctacatcat taacaataat tttgcactaa
     2401 ataacatcat tagcctatct ctgatgcatt ttctttaatt ttatttatag ttttaatcaa
     2461 tttatttctc tcttcaatat gattttcaaa taaatcaatt atacttccaa cagttctaaa
     2521 tgggtcttcc atcaacactt tgttatcctc tataaatcca ttcttgacta cataatcgac
     2581 tataagttta acaaaatgta tttgcttagt attaaagctc ttattattta aaaactctga
     2641 aaaagcttca tttgcagctt ctctatccaa tcccactagt cttctcacta attgacttac
     2701 tggagtatct ccaaactcct tcttataatc atcacaatta cctaactgtt ttaagagtat
     2761 ttcctccaac gtttcaacat cttgctttgt tatcttttta ttatttctta atttaaatat
     2821 tgctaattca tctttatggc tatttaaata atactctacc ttctttttat aattcttaag
     2881 attgtttgca ttatatacag aatcatttct atcttctgct acaatcatat cttcaaaact
     2941 tgtataatat atcttttgac tagacttttc aagatactta ataagctctc ttaatgatat
     3001 acgtacttct tctacctcaa atatatctga atcttcccaa aaattctctg tcattgcttt
     3061 ttcaatagta tatttttgtt gctgaatctg tggtatagtc ccaagttttg aaagtttttc
     3121 tgctgtagcc attacatgcc ttataccttt acttgcatta ttattttgta agtatgcaag
     3181 ttgtatatta tacataacta catcaaatct tttagccaat tcatcatcac tatatgaagt
     3241 tatgagtggt gatatatatt ctcttatctc attttgtgct actgctccaa tattgctcca
     3301 aacattttca tttttatact tatgtacata acttaaattc atcctaacta tataactatc
     3361 ctcatttaaa gcttttacag aagttataag ttcttctaac aattcttttc taagttctgc
     3421 atactcacct tctatatatt taatatcttg aagttcttta accatactta cctttatatt
     3481 aaataatttt tcagtaagtg tttctgcttt atttccctca aatcctttag gattaaattt
     3541 aaaaaattca aaattattac aaaaatcaaa tattaagaat ctctccttat ccatatcaac
     3601 acctaataaa tctggacata atctagttcc tctacctatc atttgccaaa acttagtctt
     3661 acttctcact ttcttgaaaa aaacaagatt tagtatttca ggtatgtcaa ttcctgtatc
     3721 aagcatgtct actgaaactg ctatttgagg taatttattc ttatcagaaa aatcatctat
     3781 tatagaatct acataattta tactatagtc aactgctttt acaaagttcc caccatactt
     3841 tggatacagc ttattaaatc tttcaactat tgccctagca tgctttgtat tctttgcaaa
     3901 aattatagtc ttaccaatct tttcttctcc ttctattttc aacccctctg tcattagctt
     3961 atttaaaact aaatctattg tgtcagaatt aaatagccac tcatttactg cattattccc
     4021 tatttcgtct ccaatactct catcatcatt aaatgtattt tcatattctt ccttctcttc
     4081 atcagaaagc tcatcatact taattccatc ttccataatc ttagtcttaa actcaatact
     4141 agtataatct actaaatatc catcctcgac tgccttatta tactcataag catatgtagg
     4201 tactccattt tccatgtcaa aaactgaata agtattttta tctatctcat cctttggtgt
     4261 agcagtaaga cccattaagt aagaatcaaa ataatcaaag attgacttat actttttata
     4321 aatacttcta tgcgattcat ctattataat taaatcaaaa tgaccaggtg taaacaatct
     4381 ctttccatcc ttagacttcg tatcatcaat tgcattcatc attgttggat atgtagaaaa
     4441 tatcatccta gcatcttctg ggctgtcttt gctatccaat aaattacata acgccaagtt
     4501 aggtaatagc ttagtaaaac tttttttagc ttgctttacc aatgcttttc tatctgctag
     4561 aaataatata tttttaatcc aattatgtct tgaaagtaca tcaactatag atatagcaat
     4621 tcttgttttt cctgtaccag tagcacatac caaaagagtt ttgcgttgct tattttcaag
     4681 ggcctcacat acagcccgaa tagcttcttt ttgataatat ctattggata tttcatcatt
     4741 tattgttaca gaacttaaag acttcttact ctttcttcta tccatcataa gttgtaattc
     4801 tgatttttta taaaatccat aaacttttct atctgaatag tcatcccaaa tatacatatc
     4861 aaaaccatta gtcaaaaata ttactggtct ttgactatat ctcttttcca aacaatctgc
     4921 atataattta gcttgttgtt gaccaatttt tgggtctttg ctcgttcttt tcgcctcaac
     4981 tactgctaat ggcttaccat tttcaccata aagaacataa tctacatatc ctacaccact
     5041 ttcattaggc ataccttgta cttctatttc ctcccctata tcttttccaa attcccaacc
     5101 tgcaagcttt aattctatat ttatatatct ttttcttgtc tcaaattcgc ttacttcgtc
     5161 tatcttgaaa tcatagttta cagtattaac ttttctcttt tcagttagtg actttcttaa
     5221 ttcctcattt tcttttatta cttcttcaag ctttttatct tttgaactta atctctcgta
     5281 aagattttta agttcgtctg gtctcgtcct tttctcttct ccatgaagta ataaatcttc
     5341 attaaagtct tctgctgtat attcatctga ataacaatag tctatccaac taacaaattg
     5401 atgcaaattg ttaagtgaaa gtattgcttc tcctctctct atgttcgcac tagtatgtac
     5461 agataaatta cctaacttaa ctatatattt taataaaggt aacatatcat agtctataag
     5521 ttctataaaa ctattattat gaatcaacgt actgagatta tcttgataag gcaatactaa
     5581 atcactatca ttagcatata gccattttac tgcaagctct aaagctcttc ttgttatagt
     5641 tgcacaagta gatggactaa ctagtatact tttttctgct tctaagcaag cagtattaaa
     5701 actattgaaa tttttcttct tttgtaaaaa atcaaaatta cccaaggctt ttatccccct
     5761 tttgtccctt agttaaaata aatctctcta aaaactattt cttacagctg tttcttatac
     5821 taacaaatac ttcttcattt tctgtaaaca attcgtacat atcttcaaac tgtggtatat
     5881 catatccatg agaatcatca tttctcaatt ttctaagtgt ttctataact atagaagaat
     5941 ttttaggtaa taaattgtga tactcacaag ctaaaaggta gtcataaaag tttttagatt
     6001 ttatgtttaa atcaattgat ttataataca acattgcttt tgttaattct agaaatgata
     6061 ccatatagct tttaaagcct tcttttatgt atctagccca taactcaggc atttcttcct
     6121 tatcatataa gattttgaag ttttgcaggt catacaagca agaaccaaaa tccctattta
     6181 cttcttctat tgagttccaa gataatttgt ttctaattac catacatgtc cctccaatct
     6241 aagatatcta ttacatcact gttttcaaaa tagaatttat ttatataatc cttaaaatct
     6301 ttagattcat aaataacttc accttctaaa atagcttttt taagtgctat tgtataatta
     6361 tctgtaacaa tttttaggtc tacatcaatg tctaatatat ttcttagagc ttctgtctta
     6421 gttgcaagtt ctgtaattgg tacttttttg atgggaatcc aagctatgtc tatatcactt
     6481 tctgatgtat gtttatctgt tccataacta ccaaatatta tgattgctag agtattttct
     6541 tttggaaata catttattat tgagttttta atctcatttg gtatatttct catttaatca
     6601 cctcttgttt attaggtaat attgtttagt taaataattc tcctttgaaa gctttttgca
     6661 ttaatgaatt aaagttatct tctaactctt ttaagttggt ttctattttt aattttaatt
     6721 tatccacttg cttaacaaac tcagaaaact gattttgaaa ttcaattgga ggaaccatta
     6781 ttaaatattc acctaactta ctaactgata actcaatttg attcgttgaa ccattaacac
     6841 attttctata tattgtgtct tgtatatttg taaattcaaa taacgctgca aaataatctg
     6901 aatttaagtt atttgaaata cttcttaaaa ctgtaacatg actatctgct atatattcag
     6961 aattatcaat tttgttaaat tttacacatc ttcctaatgt cccagttcca gtagaattta
     7021 ttaatatatc attttgtttc aaaaatatat cttctatctt ctcttttaag ttttcatcat
     7081 aatatttaca attttctacc ttcaaatttc tccagtatat acatttttga tttataactt
     7141 ttatatttga ttgctctaca tatttaggag atacacctct ttttaaatat atagaaatct
     7201 ctttaatctt cttcatatca aatttttttg tattattaat tggctcccca aacatctcaa
     7261 taaatcttga tttaactaat tcatccaatg cttctatttg ctcttttctt ttatttatta
     7321 actcttgagc tttatttaaa acttctacca ttttattttg tatacaaatt gtgaaatatg
     7381 gtatttttat atcatttaac ttgcttttat ttaaagttgc acccataact gctctatcag
     7441 tattattaaa atttaaagtt ctcaaagcat aatataaata ttcagatgtt ataagttcat
     7501 tattttttat ttgaaagtta gcaattgctt catttgtaaa catgtcttct tttaatatgg
     7561 caactctacc tatagacaac ttaaaactca ttacaacggt atttttaggt actaatttca
     7621 tattagcttt ttcaacagct aaatctgtta tctcttcttt tgattttgat atatattttt
     7681 ctttcaaatc agaaattgat aaccatctat taccacttcc ccaataatca gaagtatttc
     7741 tagatggtgt tttgccaata tttatatagc acaattcatt taattttata tactccatac
     7801 cctatacctc taacattctt tctaattctt ctaatccaac agctatttcc ttctctaact
     7861 ccctaactct acctataatc acactaggag catcataaac tacttcctca tacactacct
     7921 ctttatactt attaatagat aaatcatacc cattctctct tatctcttcc acatcaacaa
     7981 aaaagctttg ttctgttctt tttctatcta cttccccatc aagattatca aatctcttaa
     8041 taatatcctc tatatcacta tcctttatct catttctctt atcatccaaa gaataaccat
     8101 cagctttcat atcatagaac cataccttat cagttccacc tgtccctgtt ttagtaaata
     8161 tcattatagc tgtagatact cctgcataag gcttaaacac tccacttggc attgatatta
     8221 tagcttccaa tttatgatta tcaactattt cttttcttat cgatttatgt ccacctgttg
     8281 aaccaaacaa tactccatct ggcactatac atgcgcatct tccaccaatt tttaaaactc
     8341 ttaaaaaaag tgccaagaat aataactctg ttttctttgt cttagtcact tttaataaat
     8401 ctgctgacac tgcttcataa tctaaacttc ccttaaaagg aggatttgct aacaccaatg
     8461 taaacttatc tttatctgta ttaacctccg ataaagaatc cttatactct atattaggat
     8521 tgtcaacgcc atgcaacatc atattcatag cacctattct aagcatagtt ctatccatat
     8581 caaatccata gaacatacca ttattaaaat gctccttcag cccttgcact aagaaaagac
     8641 ttgaatgatt atcccttaag tattgttgtg atgatactaa aaaacctgct gaacctgctg
     8701 ccgggtctac tataatatct tctggtgttg gtttaataag tcttactatc atatctatta
     8761 tgtgtcttgg agtcctaaat tgaccattag tacctgctgt agccactttg gataataaat
     8821 actcatacaa atctcccttt gtatctctat ccttacttaa ttctaaatta ctaataccat
     8881 caactatttt agataacata agaggcgtag gaattttaaa tattgcatct cccatgtact
     8941 ttgcataagc tgagtcttta tttccatgta atttttttat gaaaggaaac acttcatttt
     9001 gtactatttc atacattttt tctgaatttc cttcattaga aaacttactc catcttaaat
     9061 gttgatgttc atcatcaaat atcctttcaa aagaaactcc caaaagagtt gcttctgctt
     9121 cattttttgt ttctacttca tccaatcctt taataaataa aagatatgta aattgttcta
     9181 taacttctaa tggattagtt aatccaccag tccaaaatgt ctcccaaatc ttgtctactt
     9241 tgctccttaa ttctcctgta atcatatatg actttccctc cagtgttcat aaaataattt
     9301 aaatatatat tttagtcgta cgccttaata attagtgtct tatattaaat tcaaaatttt
     9361 actacacagt tattaaatta tttttcctat ttacatgttt tagttaaaaa actaatgcaa
     9421 tactcttatt ttatgattat tatacatccc atacaaaatt attttatcat atatccagca
     9481 tagaagtcat ctacctggat aactcttacc atattataac tttttaatat taatttaaac
     9541 ttagaaagaa attttttatt taattatatc atcttcctta tttcaaattt ctcaattaca
     9601 atctcaatca aaaaataaaa taagatgctg tctcaaaata gatataatct tcttttttat
     9661 tttcggaatg atgaaaatat atttaatttc atacaaaaaa gagtgcctcc tgaactaaaa
     9721 agttcatttt gagacacccc ttaatttaat cttacttatt ctctcccttt attattaaca
     9781 cttcactatc tctattgctt ttcctacaca tccacaaact tccatcctac tcttaacaaa
     9841 atctttaact gactctaaac cttttttacc atatttctta gggtcaatta cctctgggtc
     9901 agaattaagc acatccttaa ccccattgct ataagcaatc cttaattcag ttgcataatt
     9961 aaccttgcaa atccctcttt ttatagattc cttaataact tcatctggta ttccagaccc
    10021 accatgtaat actaatggaa ctgatacaac atctctaatt tcagataatc tatctaaatc
    10081 aagctttggc tctcccttat ataatccatg tgccgtacct attgctactg ccaaagatga
    10141 tacccctgtt ctctctacaa actcttttgc ttctaatggg tctgtatatg ctcccccatc
    10201 accaccatct aaatcgtctt ccttacctcc aactttacct aattcagctt caacaggtat
    10261 atctgaaggc atacatgcat ctacaacaga tttagatatt gaaatattat cttcgtaact
    10321 ttcatgagaa ccatcaatca taatagaagt atatccaact ctaagtgcct gcatagctag
    10381 tccaaagctt gaaccatggt ctaagtgcat tgctactgga acacttgcct tctctgatgc
    10441 tacctttaca ttcgctaaat aataatctag ccctgcatac tttattgttg aaggtgttgt
    10501 ttgcaatatt actggtgatt taagttcttc agcagcctct attactgcca taaccatctc
    10561 catattttca acattaaatg cacctactgc ataatgtcct tcttgtgcat ctaataatat
    10621 ttgtttagtt gtcactaatg ccataatttc tcctcctata tgtttatact gatttgattt
    10681 cttcgttatt taattttgct actatttctc tacttgtttt gctatttaat aactcattta
    10741 aaaattcttc attcattaat tttgaagata gttgagataa tgtttttaag tgtaaattat
    10801 caccattatc tgtagctgct atcataaata taatatgtgc taagcttcca tcttctgatt
    10861 caaagtcaaa cccttctttt gatacaccta ctgctacacg tggtaccttt actgcatctg
    10921 tttttgcatg tggtatcgct atccccatac caaaacctgt agatgatatt tcttctcttt
    10981 taaggatttc ttctttaaac ttctttttat ctaaaagaac actagattta tcaagtattc
    11041 caatcatttc atcaattaca tctgttttat tttttccctt aacatctaaa attactgttt
    11101 cttcgctata taaagagata ttattagata cgttttccga ttctgcactt tcatttttat
    11161 gttcatcttt tgttacattt gtattttcta tagtattttt ctcatttact actttaggtg
    11221 taaccatcat ataaagtaca gcacctacta atgaacctac aaatatacac cctacccata
    11281 aaagtggctt attgactaat cctagaacca aaaaccctcc atgtggagct ggtacttcta
    11341 ttttcattat ataagttaaa attgatgata ttgatgatgc aatcataagt actggcaaca
    11401 cttttaatgg attttttgct gcaaaaggta ttgccccctc tgttatatga gtactaccta
    11461 gtatataatt gattaatcct gctgctctat cttcttctgt aaatctattt ttaaataaag
    11521 tagatgctat tgctattacc aatggtggtg ttatacaagc tgcagataca cctgccataa
    11581 ataaataatt tccctgtcct agaagcatag ttccagttac atatgctgct ttatttactg
    11641 gacctcccat atcaaatgct gacatacatc ctattactat tcccaaaatt atagggcttg
    11701 aattttgtaa ccccgataga aagttcatca tagcattgtt aagtgctgaa catggcccac
    11761 caagtaacaa cattgctaca cccacaagac ctacgctgac aataggaatt ataaaaatcg
    11821 atttaagacc ttctaattgt cgtggtaatt ttcttaacaa atgtacaaat agtttaacta
    11881 agtaacctgc taaaaatcct gatatgattc cacctaaaaa ccccgaacct gtatcaaatg
    11941 atatcattcc tccaacaagt cctgctaaca taccagctct tcccgatata gattgagcta
    12001 taaatgctgc caatacagga accatcaagt tcatagaata tccaccaaca gattttaatg
    12061 tagctgctat ttgattatat tgttctgaat ttgggtcaaa tgaataaatc ccccataaaa
    12121 atgatatagc agtaagtact ccacctgcta ctactaaagg taacatgtga gatacaccat
    12181 tcattaagtc tttatacaac ttttgaccaa acgataaatt tccttcttct gcaattttac
    12241 tgcttgataa gtttcctttt ctaatagcta cttttccatc aatacatctt tgtataagtt
    12301 cattagcttt atgtatacct tctttgacag gtacttctat tactggcttt ccattgaatc
    12361 tgtccatatc aacattttta tcacaagcaa ctattattgc atctgccatt tcgatatctt
    12421 ttgctgttaa cttattttct acacctatag ctccattcgt ttcaactttt atgtcacacc
    12481 ctaattccct ggctgaattt tttaatgctt cttctgccat aaaagtatga gctattccag
    12541 ttggacaacc tgttacccct agaatcattt tctttcccat ttttatacca cctttctgac
    12601 tcttatattt tttatataat cttccacatt ctccaaatta cctataccat tactttcagc
    12661 cacacttgct cctgttgctg ctgacctttt taatgcttct tcaatatgtt ctggattttc
    12721 caaccatata cttaaaaaag cagctaatgc agaatcacca gcacatgcag agctgactac
    12781 tgtaactggt tgagcactag cataataaat tgattttcca ttataaaaat aagaaccttt
    12841 ttctcctaaa gttagcaata tattttgagc accttttaaa tgtagtaatt taagtacatc
    12901 cttaatatct tcttcatctc ttacaataat tccaaatata tctttgattt cttcatcatt
    12961 tggttttatc aaatatggat tatactgtag taactccttt aattttggtg aacttatatc
    13021 gagtattgtc tttatacttt tatttttaca aattttaaat atatcctcat agtaattatc
    13081 atcaatacca ggaggtagac ttccacttat tgataagtaa cttatatctg ctaatgactg
    13141 tatcttatct agcatatcta gtttttgttt ttcatttaca tatgacccag agtttacaaa
    13201 tttaaattca tcacttccat catttaaaaa tatatttata cgagttgtat cttctaccca
    13261 agtaggcaat acgtcaaatc ctctcttttc gctttcttca actatatatt ttcctgaaaa
    13321 tcctccaaaa aatcctaata tcttagattc tataccataa tgacctagta caaaagttac
    13381 atttaatccc tttccattag gagaatatac agcatttgat gtacgattta ctaattttct
    13441 ctttatccca ttagtagata tattcatatc tattgcagga tttgtagtta atgtatatat
    13501 cattgtcttc ccccttgctt gtgctgtgta tgttttcatt ataataaaaa agtctatgaa
    13561 aatcttttca caaatagaaa atcgattttc atagactttt caatttaatt ttcagaatta
    13621 ttatatctga ccatataatc tataattatc ctagatataa tatacaaggg caatctggaa
    13681 gatacttcat attcagaaat tgatatatgt tcatgtttgt atcctacaat atataaatcc
    13741 gaatacttta taagttttgc actttcacta caacagcaag ttataacctt agctccaact
    13801 aaattggcat tctgtgctga ttctacaagc tctatggtat ttccattcaa tgaaaatata
    13861 attataagtt cgtctttttt tattgattta gattttattt tcattatgtt ggggtcattt
    13921 ataaaaacaa cattaaaatc aagtaattgc aatttaagac taaactcttt tgctacatat
    13981 tcagtaagcc ctcttgctaa aatatatatc ttactagatt tatttatagt ttttattata
    14041 ttgattagtg tagttaaaga tatttgttct ataactctct gtgcttctat aaaggattta
    14101 tttattatat cccccatatt ttgtatatca tcattttcat cttttactgt taatctatac
    14161 ctaagttcat tgaatccatt taaaccacat tttctaattg ctcttgaaac ggttgctgga
    14221 gatgaatatg tttcatgagc aatctctact atagataatt ctggtaatat tgattcattt
    14281 tcatttataa atttaattat ttcaagttcc gtcttcgtta gattttcaga attacttttg
    14341 tttagctcaa ttatcatatg ttatctcccc ttatataagt tttatttgtc taaatttatc
    14401 gagtaatata atatattacc tcttaactac ttatttttat atacaattaa gatttagtca
    14461 atgtctatat atgtaaattt ataatataat aattttttgc ttaaagttta ttatgttatc
    14521 ttatagcatt ttaaaataaa aaagaattgt acctctacca aaatttggat gaagtacaat
    14581 agatattcct catactgata tgtttatgat taaaagtata aatataaatt tcgctatgaa
    14641 tcatataatt aataaatcat tttatttaca attataaccc cccttataaa actatattaa
    14701 taatatatac agtatttata aggggggttg tttatattta aaaactattt acttttctct
    14761 taattcttct ccattagttc ttattacatt ttgataccaa taaaaactat cttttttata
    14821 acgttcttta cttccatttc cattgttatc tatatccaca tatataaatc catatctttt
    14881 ttccatttct tgagatgaac aacttactat atctattggc gaccacatac aataagctaa
    14941 tatttcggtt ccatcagcta tagcttcacc tatttgttct atatgttttc ctaaataatc
    15001 tattctgtaa ctatcatgaa cttttccatc ttcaaattta tcatgcattc caaatccatt
    15061 ttcagctatc attattggct tttggtatct atcataatat tgagataaag tattgtataa
    15121 tccttttggg tcaattgccc acccccaagg atttgctttt aaatatggat tctttatatt
    15181 gtcagttgga ttcatggtgt ttttacttgc tgatactgtt gtagaataat agtatgataa
    15241 agctagaaaa tccatagtat tttcttttaa gattttatca tcaccttcct ccataactat
    15301 atttatgtta ttttctttga aaaatctctt aatatagact ggatactctc ccctaaaact
    15361 tacatcagtg aaaaaatatt gtagcctatt tctttttaaa gcaaatacta cattttcagg
    15421 gtcacaatct tctggataag atgtacaatc tgccatcatc atacctatca tcatatctga
    15481 atttatttct tttccaagct tcttagtcaa agctgtagct atgaattgat gatgaatagc
    15541 ttgatatcta gtctcttcta cattatcaac ttggtctaca caaaatgcaa ctgaattaaa
    15601 tgattcataa tgtattaaat ttatttgatt tataactatc caatatttaa ctttattttt
    15661 atatctattt agtattaatt ctgcatatct aacaaagaaa tctaccactt ttctattaga
    15721 ccatccttta tattttaata ctaaattcaa aggagtctca tagtgaagta gtgttacaat
    15781 aggttccata ccattatcta ttacttcatt tataagatta tcataaaact ttaatccctc
    15841 ttcattaggc tctaaatcat ctccatttgg aaatattcta gcccaattta ttgaaaatct
    15901 aaaacacttt aaccctagct ctttcaaaag ctttaaatct tctttgtatg tatgataaaa
    15961 gtctattcca ctacgcttag gataatatcc attattatct ttcatagcaa actttattct
    16021 ttctatagtc atatcagact cataaggttt agatatatct ctatctttat catacatatg
    16081 tacatcagct aaacaccaac ctttgccacc ttcattccat gcaccttcta attgattagc
    16141 tgctacagct cctccccata aaaaaccttt aggaaactta cccaatttaa tcactcctta
    16201 ttttatttat ttaaatcatt tactattatt taccttatat tctatagact ttactattcc
    16261 tactaaagac atagttatat ttaactcgct gaatatggtc ataagagtat cttgagcatg
    16321 tgcaaataac agcgaatgtg ctcttttctc tccaccagct tccccttgta taatcttggt
    16381 ttgagctcta tgtgcttctg tcataaaccc ttttgcttta gctagtttta aatcagcatt
    16441 tctataatca aacttttcca aatcattaag agcatgttta atttctgttc tagcctcacc
    16501 tgcacgaagt attatttgca ttgctacttc tactaattct tcatctatta tatgttcttc
    16561 aattgtcata tttataattt cctttcaatt tctaaattta aacctcttga ctctctaatt
    16621 ttatctcttc tttctcataa gctttaaaaa atggatacca tatcataaat agcaatacta
    16681 tatttattgc taaaacaata agacctctaa catcttgatt taccataaaa gtagatatac
    16741 caaaaggaac ataccataaa ttaaacacct tacttggaat tgtaacaaat cccatgctca
    16801 ttgataaata agtagttatt gatattacta ctgttcctat ccacataggt aacattaaaa
    16861 atgggttcca tgccactgga gctccaaata ttactggttc atttatattc attatagaag
    16921 ggacaataca tgctcttcct attgctttta ttcttccaac tctggataaa cacatcatta
    16981 taactaatgg taatgtagca cccaaccctc ctaaagttat ccagcctgtt tgaataactt
    17041 cgtaaacata tatattagtt gctactcctc ctgatgctac tgctgctgca ttagctgcaa
    17101 ttgcaccaag ttgaattgaa taagttatag gagatagtac ccacccactt attcctaaag
    17161 aatatataaa tactactaag aagtttaaga gtacaaatcc aggtaatgtc tgtgctatat
    17221 ctgatactgg tttaaatact ctaactatta tttcaaacat gtcaaagttg agtccataaa
    17281 ttaatcccca gcctacaaac attatcactg ttataggtaa catattatca aaccacttgc
    17341 ttacaaaatc cggaagtgct gaatcttctc cgaaaaatga aaaagaacca aataaagaca
    17401 ttattaatcc agagaataat cctgcaacta gcgatacaag cattccttct cctccaaacc
    17461 tgttgaagtt aaatatagct ccttcattcg taatctctgg acgaatcatc attaacatta
    17521 atccagcacc tgcaagacca gctactacac tcatactttc aaatttctta ttcttcattg
    17581 tttccatagg caataaaaat gctataaata atcctaaaag tccaaatgta tattgattta
    17641 ttggacttaa atcaggtaaa aagcttatgt aattcttcaa gacagaaact atagttacta
    17701 atgagcctac aaataccatt ggcaaaactt tcattatagt tgattgaact gattgtaccc
    17761 atatattctt agtcatatta tttacaacag gagtaaattt ttcacttaaa aaatctataa
    17821 atttattcat aaacactctc cccataaata ttttatttgt ttgatattag ttctagtgct
    17881 tcttccaaag cctttgcacc atctagtctt ccatatgcag ttttagatat acatgctact
    17941 ggaacgttat attcattagc cttttgttga atttcttcct tcatataatc taaatgtggt
    18001 cctagtaata aacaatctat ttctgataaa tacttatcta tctctgactc acttctcgct
    18061 tttatatctg cttctatacc ttttttcttt gcactctttc tcatactagt tgccataaaa
    18121 cctgtacttg ctcctgaacc acacgccaaa agcactctta tatttttcat aataatacct
    18181 ccaatatttt tatccattaa tttatttctt aatgaacttt ttattttatt atgtttatat
    18241 tttacttccc aaataagttc atttcacata aatatatttc ctaataatag gaaatcattt
    18301 actcttaatt agacctatta atatagtata atgtatctta taatacgtac attcaattat
    18361 ataattatca tatcatacag attttatttt aaaactatgc gagggaatct aatgaaaaca
    18421 tctaaaaaag agctattttt aaatttcata aaaacgcaac atgattggat agattcatcc
    18481 acattagcta attaccttaa tgtatcaaca cgttcgatta gaaaatatgt aaacgagatt
    18541 aattctaatg gtgcatttat attatcttct aaaaagggct ataaagtaaa tctcaatact
    18601 aattgtcaaa ctaagattga tagttcagag aatatatcac ctgataacag attaaattta
    18661 attttaaaag aacttatagt taatagtaat ggaattaata tatttgattt aagtgaagaa
    18721 ttatttgtta gcccagctac tattgaaggg gatatagtca aagctaataa atttatagga
    18781 agttataatt taaagataaa gcaatcgaaa tttttattga aactagttgg aaatgagtct
    18841 gcaaagcgca agttaatgag ctctataata tttaaagaaa cgggaagtga ttttttaagc
    18901 ttatttgatg tacaaaaaat atatcaagag tataatttga ctaaactaaa agaaaatata
    18961 atatatatat taaaaaaata caatttattt ataaatgagt atgctataaa caacatactt
    19021 cttcatttaa tgataactat agacagaata aaaaagaata attacataga ctctgttgaa
    19081 gtagtaaact acatagacaa taacgtagat ataaatattg caaaagacat atcaaatttt
    19141 ttagaatctg aatacaatat aactttaact agtgcggaat tatactattt agtatttcag
    19201 ttaacaaaca aaactacagt cttaaactac aatcaaatgg atactaaatc attgtcaaat
    19261 tatatagatg aacactttgt taaattaact aaaaaaataa ttaaaaatgt atatgactta
    19321 tatcttatag atttatctga tgaggaattt gttgtaaaat ttactcttca tgtaaaaaat
    19381 ttaatcagta gagccaaaaa taatcaagtt ttaagaaatc aaatacctca aaagttaaaa
    19441 gattcctacc ctttgatata tgatatatct gtttatattt gcaatcagat tcaaacttta
    19501 gaaaatgtag atatagacga agatgaaata tcttatatat ctcttcatgt aggttcattc
    19561 tttgatagac aaaaattatt agaagataaa gttctatgtg cattaattac tccaaattac
    19621 tatgatttac aattcaaaat agtaagagac cttgagaaga gatttaatga atccatagaa
    19681 attattcaga ttttttcaga tactcataat cttgattttg acaataaggt agacatggtt
    19741 attactactt taccaattaa cgatagatgc cctatacctt ttgtttatgt aaatccttac
    19801 ctaaatagaa aggattatga taatattcaa agtaaattta ttcagattaa agatagaaaa
    19861 aatatactta ctgtacaaaa tcatttagaa atgtacttta gtgaatcttt gtttatgaaa
    19921 aatatatatt tagatagtgc taaagattat ataagattta tgggaaatat tctgtacgaa
    19981 aataaatatg tcaaacccaa ttatatagat gatgttctta taagagaaaa aatgtcttca
    20041 actgcattta ataacaatgt agcaattcct catagcatga aaatggatgc tttaaaaacc
    20101 ggagtatgtt taattgttaa cgataaacca gttaaatggg gtgaggaaaa agttcaaatt
    20161 atagctatga tacctataaa tgaaaaagaa aaagaaaaat tcaattacat atttgaaagc
    20221 tttattgaaa tattatctga atggaataat gttaaagaac ttactaaggc agataactat
    20281 agtagtttta tgaatagaat agcttatctt attcaaaata tctaaatcca ttaatttatt
    20341 tccaatatat gtttcttaag ttattaacat aaaatagata agctgtcaca ctaaaaagtc
    20401 taaattaata tgtgacagct ttttacctaa tataccttgt tttaatacta catatttttt
    20461 gactgttgta tactagttat aagattgaat atttgtgttg ttgtagctgt catattctca
    20521 ctagcagttg ctttatccat tgcttcttct attgtcatag ctctatttac tatagaaaaa
    20581 taagcatcta taccctcttc attacattta actgcatctt ctgttgtggc tccagccaaa
    20641 cctattactt ttaccccata tttttttgcc agttttgcaa ctcctattgg agccttgccc
    20701 atagcagttt gattgtccaa tctaccttca ccagttatga caaaatctgc atcttttaac
    20761 tcttcctcta atttaatttc ttctaatatt atttttattc cagattctaa ttttgaatct
    20821 aaaaatgcta aaaaagcaaa acctaatcct cctgctgctc ctgctccttc tacattagct
    20881 acatctttac ttaagtaatt tttaactact ttggaaaaat ttctaagccc attatctaac
    20941 ttctcaatta tttctgatgt tgcacctttt tgagacccgt atatgtatgc agctccattt
    21001 ttcccaaaca gcggattatt tacatcacag gctattttaa acttacattc atctaattcc
    21061 tttaatctat tttctatttt tatatgtctt atttcattta aaacttttcc acctaatcct
    21121 actaacttgt tatttttatc ataaaactca aaccctaagg cttgaagcat acctatccca
    21181 gcatcattag tagcacttcc acctatccca actataaaat ttctatagcc tttatttatt
    21241 gcttccttta ttatttcccc aacaccataa gttgtagtgt ataaaggatt tctaagttca
    21301 gtcggaacca atgttaatcc tgatgcttga gctatttcta ttattgctgt acttgtattt
    21361 tttaataatc catatgttgc atccactttt ttagctattg gtccagtaac ttctatagtc
    21421 tcttttttac cattcatccc ttgaactaaa gcatctactg tcccttcacc accatcagca
    21481 agtggcaata tctttacttg cgcatcttct ttaaccttca atatcccctt ttttattgca
    21541 tttcctgcct ctattgaaga taagcttcct ttcaaagaat ctatagaaat taatattttc
    21601 atacttatct actccttttt attactcctt attttaaaca tataatttta taagcttaat
    21661 ttatctttta ttttatactc tacatctctc tttgtataca acttatacca ttaatattct
    21721 tttatcttat aaaaagggag tgtctcaaaa taaatttctt agttcatgag acactctttt
    21781 gtataaaatc aaatatatgt ttattatttc aacaatgata aggaggatta tctctatttt
    21841 aagataattc ctttttagca ctatctaatc atttttagca ctatttaacc agtatagttt
    21901 taacttggtt cattaagcac tctacattta cagagttatt atctattgat atagattcta
    21961 attctttttc atttaggtta atccatttag ctgttttaat atttttgtta aatactatgt
    22021 tagctgattt tgtttcttct ttagaattat aaaatctaac tactaatgcc tcttcttttt
    22081 cagccttttt tacaacactt aacacaaggt ccttattagt ttccttaagt aaactatatg
    22141 aataatctac ttctatttct gattgattca atttcatagc attatatggc attttattat
    22201 aagtttgtat ttttgttaaa tactctttag ccatatttgc aacatttgct tctagtgtag
    22261 atttttcatg agtaactatt gctaagtcta tttttatatc tcctatcatt tgagaatctg
    22321 gagtaggcaa ctttattcct gatggacgac ctgggcgtct tgccatttct tctttaccta
    22381 ggaatcctac acttctaaat aatgttatag ctattgtgtc aaatttttcc cctactattt
    22441 caaactctct tgtactgttt gtaagaacag ctactccatg ctctttgtca gacaatccaa
    22501 cgaatgttag cattggatat atagaatctg gtctttcact ccagtcttcc tgttgccaaa
    22561 cttccatagc atcatcatac acacttctct ttatatgtcc aaactgatta tcagacacag
    22621 aaaactttga agctatattt gttggtatat aagttctaac cctacaatct cttgcttggt
    22681 tatttatgtt gaatactaca tctattatag ctttatcatt tggaacattt ataactatat
    22741 caaagtcaat aaatgaatct gctctattat tttttctagc ttgtatatct tttggtactt
    22801 ttaatctata atttatcttt atctcattat taaatctatt tttctttata gtggaatttg
    22861 ctactatatt atcgttaaat attaatttct catttggaag tggtgaaaaa tcatactcat
    22921 ctccttcatc tcctccattt tccatcaata atacattttc aaaagtttta tttaattttt
    22981 tatcaaatat ctttaatgtc ccgttttcat taacttgaat agtataaaag tcattatcta
    23041 ccatgtcaca tacttcataa gctttttcta acatatgttc aacttctttt atcatatacg
    23101 ccttataccc cattgcaggt attttatctt taaagtttat tgtatatacc ataaatgaat
    23161 catagtttcc ataatgaaca atttgacggt ctattaatcc tgcatctatt acttctttat
    23221 gtaccacttc aaaatccaac ttattattat ctttatcaaa tatttcaaat gctttcattt
    23281 tagttataac ttgggcgctc actatttcat tcctatcgta aggcattaag ttaaatgcta
    23341 ttaacttatc taaagccatt tcacaactca ttgcatcaac tattcttctt ttgtaaaatg
    23401 ttattaattg gtctactttt tcctcagcta agaaaaatct attcattatt tcctggtgaa
    23461 ctttatctga acaacaacat ccaatgctgt catgagcatg atttttcatt atttctttcc
    23521 aaattaactc tattaatcca tgatgatatt caaatcctag agaatatgca atagaagcta
    23581 gtggctccaa tatgttagtt atctttgatt ctattctagc atttgctgat tttatatcca
    23641 ttcttgttga gaatatactt ctatgaaccc tcatatattt cccatctaaa aattcccctt
    23701 ttattgtatc tatatcctca tttttttcta gctctttaaa tatgttttca tatctactta
    23761 agaaaaattt tctgtctgga taacattctt ttaatttata tataacttca aatatattct
    23821 tttgaattgg catttggtca tgtccatttg gaagtattat atggtcagta gttgcaccct
    23881 tatcaagaac cggtaaatat ttatccattc ttgttcttag ctcatcttca ttcattggta
    23941 gatattttcc tattgcataa cctaatggaa gtatttgaac caaaacagaa gacccatcat
    24001 cactcttcca attaaactct gttttattag ttccttttct ttcagaacat cctctccaaa
    24061 acattgaata ttttatatca aacccattta gtatttgtgg catttgagca ctttgaccaa
    24121 atgaatctgg taaatatcct attttcatat attctccaaa ttcatcacaa tcttttattc
    24181 catatagtaa atttctaagt atggattctc caccaactac catttcatca gtttgagtat
    24241 accaaggacc tattattagc cttccttctt gaactaattt ttttattctc tccttacatt
    24301 caggcttaac tgctaaataa tcttctaata tagcagtttg accatccatc acataatatg
    24361 gatagttatg attattttct aacatatcca ttatttcttc catattattt acaagtaata
    24421 ttctagattc ctcagttgag aagtaccatt ctctatccca atgcatatga ggaactatgt
    24481 gtacattttt tttcattgct tatcctccaa aacttatctg ataatatttt ctgtctaatt
    24541 aaaaatccaa taaaaatact actgagttta caaaattttt taatatttat tttatcacta
    24601 taattaaacc tttgatttaa atatctaata acgtatcttt taatgtatct ttctttgatt
    24661 tttttgtttc ctagttatta ttaataatat tgctgatatt atagctccaa gtactgccgc
    24721 aaagaaccat attgatgcta taataaatcc tgacatactt ccctctaaaa gcgccaatga
    24781 gaatatcccc gctccaggca cttgaagacc tatattgaag aatgctacta tagacccagt
    24841 tatggctgaa ccagctacca atgatggtat aacacggatt gggtcctcta tcataaatgg
    24901 tattgcacct tcagttattc cagccaatcc taatatccaa gtttgctttc ctatttcctt
    24961 ttctgattct gaaaataaat ctctgcctaa aacagtagct attgtaactc caaaagctga
    25021 aaccatctta actgatgcaa atattgcata aggtataaaa tttccacttg ctattgctcc
    25081 tattgcaaat gtataggctg ctttatttat tggtccacct aagtcaaatg aaaccatagc
    25141 tcctattatt gcacctagta gtattgcatt tactccagtc atcccattta accaatttat
    25201 aagaccattg ttaagtgctg ctacaggctt tcctactaca aataacatca ataaacctac
    25261 taccaaagaa ccaactactg gatataccca aaaacttacg aatccagcta atgtgccttt
    25321 ggctggtata ttcttcttta aaaattttac tatataacca gcaagtaaac ctcctaacat
    25381 acctccaaga aatccactac ctattaaatc agctgctaga cctgctgcaa atcctggtcc
    25441 aagacctggt ttttcagcta gtgagtaaga catatatgct gataatactg gaaccatcaa
    25501 tgtacctaac atacctccag aaagctttct taattgccat aaccaagaac cttcttgatt
    25561 gtatagttct tgtagaccaa atgcttgaga tgctaatact gcaaatgcaa gtatcatacc
    25621 tcctgccaca ataagtggta ttatatgaga tactcctgta agtattgctg attttacatc
    25681 ttttttccat gatgattttt cacaatatga tatatcagat gatttttcta tataatcacc
    25741 tttaccttgt ttttgtgcta cttctaaagc ttcgtatatt atttcttttg ctctttttag
    25801 tggagctgct actgacgttt ttatgtttgg caaatgttca aatctttctg tatttttagg
    25861 tgctacatct gttgcatata ttattgcatc tgcccttttt attatatctg gcgtatgagg
    25921 gtccaccata ccattagctc cttgcttttc tacatacaca tccacaccta tttctttacc
    25981 agctttaatt aatgcttctg ctgacatata tgtatgtgct ataccggcag ggcatgctgt
    26041 tattgctaat ataatttttt ttgattcatt ttgaatccta atctcttctt ttatttgttc
    26101 tttcttttct tttatatcta agttattaaa taactctttc ttagtttttg aatttaacaa
    26161 catattcata taatcttcat ttgacagtct agtaactaac tcagaaagta tttgtatgtg
    26221 tgtatttcca ccttcacttt ctggtactgc tattaaaaat attagctcta ctttattatt
    26281 tgggtctacg ctttcccaat tttctattgg tgtctctaag gtcatagctg caaatgcagc
    26341 ttctttaact gacttagatt ttccatgagg aattgcaagc cctctttcaa aacctgttgg
    26401 agataaagtt tctctgtaca ttacagcttc taaatattcc tcataagatg ttaacttgcc
    26461 ttcatcatat agctttcttg ctaagaactt tattgcgtct tcctttgttt ttattttttg
    26521 ttttaaaaaa acaaggtttt catttgtgat tttcttaaat tccaaaatta cgccctcctt
    26581 aaatcattta atgctatgaa tatagcataa aataaattat gaaaatgttt ttttgaaatt
    26641 atacacacaa tttcttctta aaaaataaag atttgtgtat ttatacacaa atctctatct
    26701 taaccaatat tgctctatta ttgctctcat aatcatcata attggaacct tatctgatat
    26761 attgtaccca tttacttgtt taattgttga atagcaatat aaacttatat ctgcaatttg
    26821 ttgcaattga ttttcacata tatgtgttaa agctactacc ttacaaccct tattaactgc
    26881 tgtctttgct gcttctataa cttctttcgt ttctccactt aaactaagtg caaaaaacat
    26941 atcttgttca cctaattttc ctatatcatg aagtacttca tgtctttgac tatagtaagt
    27001 tatattatca tgcccacctc taatttcttt tactaatatc tcacataaag gaatgttttg
    27061 acctattcca taaactataa tactcctaga tttttgaatt atatctgcta ctttatttat
    27121 tttttcataa tctattagct ctaatgtctt ttttatatct gattcaaaac cattttttgg
    27181 ctctttatcc tcatttttta actcttcttt taaattattt tttagttgag agaaaccatc
    27241 atactccatt tttttacata gcctaactat ggtatttggg acagtaaaaa atttatctgc
    27301 taaagattgt attgataatt ttactatttc ttctttgttt tttctcatat attcaattat
    27361 catgtcttct gtatctgtaa atttatactc atattttttt gctctttcat aaaattccat
    27421 agtttctcct ccatctattt tattattcat aagtaaaatt caaaaatatt agctaaaatt
    27481 ttcgttaata taattataat ttaatatttt taatttatta atgcatacta ctctttttat
    27541 tatacataaa aaatgtgtac ttcctatatt gatagagatg attcaagttt ttattttgat
    27601 acatctctat caatattttt ttagaaaata cacatttttt tcaaatactt atttttaatt
    27661 aaaactttag gtaatctatt ctttatatat aattattaga cttgaaaata tctttaaaat
    27721 caaccaattc aatattattt ctttttaacc acattttaac ttccttatca catagcattg
    27781 catgttcata tactctctct tgaataagag aagatgattt tataagacta taatctataa
    27841 atcctggatg agtaaccatc aatgttatat ctttatctaa atttatatat ttttcttcaa
    27901 ataatttaac aaaagattta tttaacttgt aaaaatcatt ctgaggatat ttacttggca
    27961 tgctaataca tgaatccaaa taatagggac aacttggtat accgtattct tttgcagcat
    28021 cacacattgc tgatattgat acatcttctt ctataccatg tatatcaata tactcaggca
    28081 atttactcat atacaacttg taagtttcaa tttgagcaca aacttcttca tatacttctt
    28141 cataattaaa aacctcttta cctgtcagta gttgcgttct tcttattttg ctagaaacaa
    28201 attttccacc ttcatctaac aaattcttta tcttaatatg ttctttgcaa caaggttctc
    28261 ctattactaa attaacatgt aaaccaaaac atacatcatc attttttacc atttctatag
    28321 cttgtcttga aaaaggcata ttcaccatga ttccagcact tgtaacaata ccgttcttat
    28381 aagaagatat tatccctagt gtaacacctt ctgaatatcc tatatcatct gccctaacta
    28441 ctaatttctt cattttatgc atctgcctgc tttctcaact catcaagttg catttccatc
    28501 tcatccttta ctttaacaac atccgttcct atgatgattt gaactgatgt attactcaat
    28561 ttaattaccc ctttggctcc tgttttttta atattatcat cattcaaaat agtggcatcc
    28621 ttcacttcta aacgtaatct cgtcatacaa ttattaagag tcactatatt ttcataacca
    28681 ccacaatttt taattatctg tttagccata tatgaaaaat taccatgaga aagttttaaa
    28741 ttcttttctt tttcactaac tttatcacct atctctatct caacttcacg tcctggtgtt
    28801 tgcacattat cttttattat tatatattta aatgttacat aatataatac aaagaataca
    28861 actcctacag gtataactaa ccacgcttta tctccataac taaaacttaa gacatagtca
    28921 attatacttg ctccattaaa tgaacccaag cgagcaccca ataaatatag tacaactcca
    28981 cctagaccag aataaaaaga atgaattata tataactttg gagatgcaaa taaaaacgaa
    29041 aattctattg gttcagttac tgttgccata aaagaagtca atgctccagc ggtcattgca
    29101 ccttttactt cttgcttctt tttatcttgt gctgttttat atatagcata agaaatagca
    29161 ggtattgaaa acatcataat tatgtaaaat cctgtccaga agaatcctgc atttgggtct
    29221 cctgccataa atctaggaat ttctcctctt ataacttctc ctgttgatgt tgtatattca
    29281 cccatttcaa ataatatgta agcatttaaa acttgatgta acccagttgg tattagtgca
    29341 cggttacaga ataaaaacaa gaatattcct attgcaccag ctcctcccaa ccaaaaacca
    29401 aattgattca ttgcagcttg tggatatatc cataataaac taaatatata tgataaaatt
    29461 aaagcaacca ctggtgatag tgtaattaca catttttctc ctgcaaagaa gcttaaaaat
    29521 gctggaactt taactttata tgttttattg taagtccatg ctatacaagc tccaacaatt
    29581 atcccaccaa atacacctgc atttaatgta tcattcaata actctaaatt tgacttaaat
    29641 atcatatatg caactacggc tgcaaccatt acacttgtct tgtctttaga ctttgagtag
    29701 gcactagctg ctcctaataa aaataagtag tccacatttg aaaatactat attagatata
    29761 ttagatatag ctggcacatt gagcatattt ggaccaaata ataatcctaa tatacctgct
    29821 gctggcatta cagctattgg caccagcata gtctttccta aaagtgacag ttgttgcatc
    29881 attttgttta agaatctctt catatccaat cccctttcta tttcggttta attttttgtg
    29941 aaaacgattt attcacattt tatattttaa atattatatt tgtttaaaca aattgtcaat
    30001 attttttatt cattttattt ttatcattta gtttaacata taaatccttt taagtgtatt
    30061 gctatatcta gtctattttt ttaattcatt ttttttattt taaaaatatg tttaaccatg
    30121 tatttgaaac aacccacaaa ataattctat tttgtccttt ttatggttga tatgattcta
    30181 ttttcactat acatgtctaa acacatttga tataatttgt tcatatcagt tcaataatat
    30241 tactaaagac ctttaaggag ggataggggt atatggctaa aaataaattt tcagagatat
    30301 atgaagtatt gaaagaggaa atacttgatg gtaaatatac aagtaatatg atgctgccta
    30361 ctgaacttca acttattgaa aggttttcat gtagcagaaa cactgtacgt agagcaattt
    30421 ctcaattaaa cacagaagga tatgttcaaa gtataaaagg caagggtgtt gtagtgttag
    30481 aaaatagttg ttctaatgat ttttttctaa atatgcacaa tttcaaaggt gtagaatcga
    30541 ttgtagaaga taaaaaggtt aatactgcta ctagtgttct ccacttcagc aaaatcttaa
    30601 ttgataataa gttatctaaa aagacaggtt ttaaagttgg tagtgaagta tattaccttc
    30661 acagactaag atatattgat aacataccta aaattctcga cattaactat tttctatgta
    30721 gtatagttaa agaccttgat gtttctatag cacaaggctc tatttataaa tatatagaag
    30781 agtgtctagg tacaaaaatt gtttcatcaa gaaagatatt taaaattgaa aaagctacag
    30841 aacttgagtt aaaaacgcta cctctaaatg attataattg tgttggtgtt attaaaaata
    30901 gtgtatatac tgatgatgga aagctttttg aatatactga atcaaagcat acacctgaaa
    30961 catttgtatt catggatgtt actcaaagat attaaaaaaa ttatgtttaa ttaaacataa
    31021 tttttttacg tttagacact ttatcccata ctaacctcac tattctatat acaattatat
    31081 gtttatacaa ataattattt attgtatatt ttagctttaa tattagttta acgcttgaca
    31141 atttgtttaa acatattata attaacttat aaattacagg aggtatcaag tatgaaaaat
    31201 tggtggaaaa aagcaactgt ctatcaaata tatccaaaaa gtttcaaaga tagcaacaat
    31261 gatggtatag gtgatataaa tggtattata gaaaaattag attatctgta ttctttaggt
    31321 gttgatttat tatggttgac tcctatgtac gtttctcctc aaagagataa tggatatgat
    31381 atagaagatt attataatat agacccaaaa tatgggacaa tgagtgactt tgaaaaactc
    31441 ttaaaagaag ctcataaaag agacataaaa ataatgatgg atatggtcct aaaccataca
    31501 tctactgaac ataaatggtt taaagaatct aaaaaaagca aagacaatcc atatcgtgat
    31561 tattatttct ggaaagatgc taaacctgat ggttctgttc ctaataactg gatatccaga
    31621 tttagtggaa ctgcttggaa atatgatgaa accactaacc aatattattt acatttattt
    31681 gaagaaactc aagctgattt aaattgggaa aatgaaaagg ttcgtgaaga atgttacaaa
    31741 atactagaat tttgggctga taaaggtatt gatggttttc gtttagatgt agtcaaccta
    31801 ctctccaaaa cacctggatt acctgatgac cctataacag gaccaaaagg agatggaaga
    31861 actcactacg ctgatggtcc tcgtatacac gaatatttac ataatatgaa tcaaaaagtt
    31921 ttcaaaccta aaaatatagt tactgtaggt gaaatgtcat ctactacacc tgaagagtgt
    31981 atcaactata ctagagaaaa tcgagaagaa ctatctatgg tttttagctt ccatcatatg
    32041 aaaacagatt ataaaggagg cgcaaaatgg actaatgaaa tgttctctct ggataaatta
    32101 aaaaaagcac aatctgactt tcaatataaa atgtatgaag gaaaaggatg gaatgctcta
    32161 ttttactcta atcacgacca acctagagct ttatctagat ttggagatga tagatttttt
    32221 cgtcaagaaa gtgctaagat gttagccata acattatttg gtctacaggg tactacatac
    32281 atatatcaag gtgaagaaat aggtatgaca aatgcctact ttactaaaat agatgaatat
    32341 gatgatgctg aaagtaagaa tgcatactat catatgatta aatctggtat tgatgaaaag
    32401 gaggcattgc ttatacttca acaaaaatcc agagataatg cccgtactcc tatgcaatgg
    32461 gataatacta tttataaggg gttttctaat cataaaccgt ggttaaaagt taataatgac
    32521 aatattagtg tagaaaatga gcttaatgat tctcgttcag tattttatac ttatcaaaga
    32581 ttaatcaaat atcgtaaaca atatgatatt tttacagaag gtacttatag attgttagat
    32641 gaaaatcata aaaatctatt tgtttatgaa agagagtata aaaaccaaaa tcttcttgtt
    32701 ataagtaatt ttactcatga taatgtagta tataaattac ctgaaactta tttgaataaa
    32761 ttaaactaca caatacttga aaccaattac actagagata taattgaaaa taaaatggaa
    32821 ataaaacctt acgaaagtat aatgatatat tataaataaa gttatttatg taaaaatcgg
    32881 tataacaccc tggtgttata ccaattttac taaattcaaa gtaatattta tagaatatct
    32941 atagattaca ataattattt gacttctaca tcattatatt tctttgaata tactttagtc
    33001 atatagaaat atataggaac tacaattatc atcccaccta atccccaaac tatcttcata
    33061 ggttcatcaa tacctgcttt tatcagtaat aatatactta taataactgc aaatattggt
    33121 ataattgctc ctccaggtat tctaaaattt acatctttac cagcatactt ctttctcaat
    33181 attagtacag ctaaacatgt aggaatatat tgaataaatc tagatacaac acttatttga
    33241 gataatgttg tgaatgaacc cgaccatgct attagtaaag ttcctatgac agatactata
    33301 attgctatat aaggagctcc atttttatta gtttttctta tagacttagg cattaatcct
    33361 tgttcaacca aagcagcagc agaccttggt gtaaatatgg atgatgcaat atttatacct
    33421 ccaacagata ctatagttcc tattgtaatt atatacccac ctataggacc taacatctta
    33481 atggcagcat ctgcaacagg agtagctgac cctgctaagc cattacccaa cacccctata
    33541 cttattccca gtataagcat atatattact gatactacaa acattaccat taccaatgct
    33601 ttaggtacat ttttcttagg gttctccata tcttttgctg caacagctaa agattcaaat
    33661 cctgtaaaag cataaaataa agttatagat actgctacaa aagctgatga cgcactttga
    33721 cctttttgta ttattacaaa tggggtaaaa ttagaaccct ttataaagaa taatcctaca
    33781 gctataaata aaactattgg taataatttt cctatagtca tgatattatt taagtgctta
    33841 gattgtttca tccccataaa atttataatc ccaagtccta caactaatat accaattatt
    33901 atatttttag taagtggttc tgctgcttgt ggccatacag atgatagtgt tacagcaaag
    33961 aaatttgcca tagtagccca agcaatcata ctaactatcc acttcataaa tccaacttca
    34021 aatccaacaa aatctccaaa tgcctctttt gcataaagat agggccctcc atcagtatca
    34081 aaccaactac ctgcttcagc aaaacataaa gcaatagaaa cagctaaaag catacaaaaa
    34141 actaatacta atatacttgc tggacctata tctgtataag catcaccagg taacccgaat
    34201 atacctgacc ctattattgc attgattcct aatagtacaa tactccaaaa acctaactta
    34261 atgccttttt ccattgttat tccccccaaa gccaacttaa tatatttgta ttttttattt
    34321 agatgcttct ttaactattg ccataaatat ttttttcata ttatcataat ccctagtcat
    34381 catttctgga tgccattgta tccctattac aaactcactt ccttcctttt ctatagcttc
    34441 aattaaaccg tctttagatg tagctgaaac aatatatccc ggtgctactt tgttgacaac
    34501 taagtgatga aaactattaa ctaaaacttc tttttctcct aatatttcat ataattttgt
    34561 cccctccttt atctgtactg tatgtgtagg tacattagat agatgttgct gattgtgttt
    34621 aatataagca ccttcaatta aagataaatc ttgatacaaa ctacctcctt cagttacatt
    34681 tataatttgt tctcctctac atatacctaa aactggtttt ttcatatcta aagcatgttt
    34741 taataatttc atatcaaaac tatctctctt tggtgatatt gcccccaact tattatgagg
    34801 ttcttctttc catatgagtg gatttacatc ttgtccccca gatagtataa gcgcatctat
    34861 attagatacc tgctccttta taatctcatc atcatataca atcggtacaa tatatggtat
    34921 agctttacac ataactactg attgtatata atcattattt acataagccc tttcatagcc
    34981 aggaaacatt cctccttcat caattagtaa attacctgat attcctatta ttggatattt
    35041 ctttttagcc aatttcttca ccccttaact tatttgtgaa caataaaaaa tgctacttct
    35101 cttaaagagc aagtagcata ccaagatttt atttatttga acattataat ttattttaga
    35161 aatacttttt ttgaatacga gtaatataaa taactttatt tacttatatt ttattttaat
    35221 cctgtttagt ttgacttttt taaattgttt taagtacaac taataggtaa aataggcatt
    35281 tagattttcc tttttatcaa cctattaaat actgtttaat atattttttc cccttctatt
    35341 taattcactt ccctttctac cagattcaca tattatcaaa tccagctctt ttaacatatt
    35401 aagtattccc cttacctcac cttctgttat attgtagcct aattcattta ttgaattaac
    35461 aataccattt ctacctatat ttttattaag attattggca ttttttaaac actccattac
    35521 taactttact ttctctatag atgttctatc ctttaatata cttatttctt tactaaaatc
    35581 ttcagttata tttaataagt tatgaggtaa atcatttatt aaaataacat cctcagaact
    35641 cataatgtta atatacatag cagtattttt taattctctt atattacctg gccattcata
    35701 tgcattaata atgtcttgaa catcttgact aattgctctc ttccttttca taaaatgctt
    35761 taacataact tctatatctt cttttcgctc tcttaaaggt ggtatattta tgggcaatac
    35821 atttatccta tagtataaat ctgctctaaa tctatctcct tttatcattt ctaataaatt
    35881 tttatttgta gccgcaatta ctctaacatc tatatctatt acattttctc ctccaaccct
    35941 cattacttga ttctcttgta atactctaag taatttgctt tgtaagtata taggcatgtc
    36001 tcctatttca tccaaaaata tagttccatt gttagcctgt tcaaataatc cttttttacc
    36061 tccttttaaa gctcctgtaa aagcaccttc ttcatatcca aaaagttcac tttcaagtaa
    36121 attttctggc atagctgcac agtttatcgc tataaatggc tgactatgtc ttggtgatgc
    36181 attatgaata gactgagcca tgagttcttt tcccgttcca ctttctcccg ttataagaat
    36241 tgtataatca gattcagcta ttttcatacc taatttctta gttctttcca tcttcgttga
    36301 aactgttttt atatcatcaa aagtatatct agcaacttgt cccttttctc taagcttctt
    36361 tgataggttt tgctccaatt ttcttatgta agttatttct tgaatacaaa aataatatcc
    36421 agtaaccctt ccaaaactct ctatattata cttatttacg tttaagtact tattattaaa
    36481 ttttataact tcatcatgtg ccttactcaa actaaaaatc tttttaatat tctcattctc
    36541 taattcatct atatttttcc ctataatatt actttgaata tctagtatat cccctaaact
    36601 attattgcaa ataattatgt ttcccttatc tgataccatt gccataccta agtttgataa
    36661 atttattata gaatttaatt catctgcttt tatagtaagt tctttatatt tagtatttat
    36721 tcctgaatat aaactaatta tttcatctgt atacttaatt agcctttcag taatttcttt
    36781 atcatccagt ttgagttttg ctattatttg aataaacgtt gaactatcaa tatacctatg
    36841 gcctaaatca ataacttctt ctatatattt tggaactaaa cttgcctctc ctggcgttat
    36901 agctatctta atatttctat attcttcatt gggattgtat ggtataaaat tcagatgatt
    36961 tattcctata ttgtataaat tagatattgt ttccaataca gtatgcttat tatcgtttac
    37021 tactaataca tctattcctt ctggcaatga aaataactta tatatatcct tttgctttat
    37081 gctcctattt atagttatta tctttgagac atcttctaca tattttttta tttttaatac
    37141 tctatcatct atcattacca atattatgtc atcttttatt aattccttct ctgttatttt
    37201 atttaaataa taatttttta tcattacttt atctttaaat atcatttcta gatttttatt
    37261 taagaaacat tctaatttag acaacccatc agttactact gctattgttt tcatttgatt
    37321 actcctttta aaatatactc tactctttta taatatacat ctgattttca ctttaacagc
    37381 acaaaaaatt gtacatttag tatactaaca tattccttta ttagttttat gtccctaaat
    37441 atcatagttg caatatctat catatttctt aaacttctat aaagcaaaaa ttgtgccaaa
    37501 ttaataattt tcttaatttt attctaaagt tcctcaatag tatttacaga cttagcactt
    37561 atagcttata agtctttata ttattaatct atttttatag gttaattgta caaatcatat
    37621 atcactttta cccattaaat gcattataaa aaagatggaa taacgattcg tcactccacc
    37681 ttttttgtaa taaaaaattg tatatataaa cactatttac tactcttcta agtacaaaca
    37741 ttatctacat actataacct ttcaccatta gattctatta cttgtttata ccagtcaaaa
    37801 ctctttttct taatcctaga caagtctcca cttccatcat catacttatc cacgtatata
    37861 aacccatatc tcttggccat ctcaccagta gatgcactta ctaaatctat acatccccac
    37921 ggagtatatc caaataaatc aacaccatct tctacagctt ccttcatttg ctctatatgt
    37981 gcttttaaat actctatacg atagtcatca tttatcactc cattagattc ttttttatca
    38041 taagctccta aaccattttc aactaccatc ataggtattc tatatctatc ataaatttca
    38101 tttagtgcat atctaagccc ttcagggtct atttgccatc cccattcact agacttaaga
    38161 tatggatttt tcactccacc taatatgttg ccacttgtat tatcttcttt agaatcacta
    38221 cttacacatg tagacatata ataactaaat gtataaaaat ctacacaacc ttcttttaaa
    38281 atttctaaat ctcctttttc catttttatt tcaatattat tttctccaaa atatctattc
    38341 atataagttg gatattcgcc acgcacttgt acatctccac agaaataatt cattactcta
    38401 ctatctcttt gagttttaac tgcatcatct ggattacaag tataaggata cattgttgca
    38461 aatatttgca tacatccaac ttgataattt gaatctattt catgagctaa cttaacagcc
    38521 ttagcacttg ctacaaattg atgatgtaac ccttgaaaac gaagttgagg tatatctact
    38581 tggtttataa aatctgtagt cccttcattt aatattcctt gtgataagta gcctcccata
    38641 ggcattgtag ctgaatttat ttcattaaat gtcagccaat attttacttt tcctttatat
    38701 cttttaaata ttgtttcaca gtaattcata tacaagtcta ttacatctct tgaaacccat
    38761 ccattgtatt tctctgttat tgcaaatggt atttcatagt gagatattgt tattaatggt
    38821 tctatattat attttaaaca ttcatctatc acattttcat aaaatttaag tcctgcttca
    38881 tttggttctt tctccattcc agttggaaat attctagtcc atgctattga aaatctaaat
    38941 accttaaatc ccatttctgc aaataaagct atatcttctt tgtatctgtg ataaaaatct
    39001 attgcttcat gacttggata aaatgttcct ttttctataa ctctcgttat tcttttactt
    39061 actttttgag aacctccagt acacatatct gaacaagaag gacctttccc atctaaattc
    39121 catgctcctt cacattgatt tgctgctgtt gctccacccc ataaaaaatc ttttctaagt
    39181 gccataattc tacctccaat aatttattta tagtaacttt attttaaaat aaaaatacat
    39241 atatataatt cttaattaat agaactctct tttacgctaa aacagttatc aattcctgac
    39301 tattatttac atgcttttgt gtattttcta aaatatccac ataatcagaa taattagtaa
    39361 caactactgg tgtttctaag caatatcctt cttttgaaat actttctatg tcaaaagtta
    39421 ttaatatctg acctttttta atcttatccc cttgattaac aaaagatttg aaatatttac
    39481 cttccaactg aatagtattt aaccctatat gtatcaatag ttcacaacca ttatcagata
    39541 taattcctat tgcatgtttt gttggaaata aagtcattac agtcccatca aatggtgcca
    39601 ctacttttcc ttctttaggt gttattgcta caccttttcc caatatacct gttgaaaacg
    39661 ctgcatcttg tatctgctcc aaagccttta cttcaccttt tattgggcta tatactactt
    39721 ctcttttgtt tttttctata acgccttgcc cattatcatc tttagaaatt tctactatag
    39781 catcatcttt ccaaaaaaat aatgttaaaa caaatcctac tacaatagct actattattg
    39841 ctattatagt tgcataaaca cctttcatat ctccagttgt tggatttata taattaggta
    39901 taccaaatat tccaagtcca cccattacat aactttttac attcattatc cccattacag
    39961 ctcctgccac accaccacct acacagctat atatgaatgg tgtttttctt ggtaaagtta
    40021 ttccatatat tgcaggctct gttacaccac atatccctga aataactgca ggtatacaca
    40081 gctcttttat cttcttatct tttaatttaa agtacattgc tgctactact gctgtttgtg
    40141 caaaagatgg tacaaatgct cccactaata ttgtgtcaaa ccccatagtc ataaggtttg
    40201 acatagctat tggtactaat ccccaatgta gaccaaatat tacaagcact tgccacaata
    40261 aaccagttaa tgcaccacat aaaattggtg aaaagttaaa taaagctaaa aatccatttc
    40321 caactatatt agaagcatag gtagctatag gccctataac cataaatccc aatggtaaag
    40381 aaactaataa aactgccatt ggtacaaaga aattctttac tacatctggt attattttag
    40441 caaacacttt ctccaactta gctgctaata atactattaa aattactggt actactgttg
    40501 aagtgtagtt cattgctatt actggtaatc ctaagaaagt tatatatact ggtgattcaa
    40561 atattgttcc agtaaataga ttgtataaag gttgacctgt agatgctaga gtatctaatt
    40621 gaatacctga atagcataaa actgcaccta taagcattcc aataaatggt tttaatttga
    40681 atttcttagc agctgtatat ccaagaatta ttggcatata ctggaacatt gcatctccaa
    40741 ttgcatttaa cataatatat gcaccatcag tttctccata caaacccaat gctgtaaata
    40801 ctgcattaaa tccttttatc ataccagcag cacacagaac tcctaatatt ggttgaaaaa
    40861 ctcctgatat tatgtctata aacttattaa gtaatccttt tggcgcatct gagtctgaat
    40921 tatctattgt atctcctcct aatccagcca aatcgcacac aacttcataa acatctggaa
    40981 catgattacc aataactact tggtattgac ctgcactttt cattacagta actataccat
    41041 ccattttttt tagcacatca tcatttgcta ttgactcatc ttttaattta aaacgtagcc
    41101 tcgttataca gtgagataaa ctgctaatat tatcctttcc tcctacattc gcaatgattt
    41161 cttttgatag ttgttcatac tttctcattt tttctcctcc attttctttt tgaaatatga
    41221 aggtttggcc aacttaatgt caccacccaa tatatataaa cctaattatt aggatttata
    41281 tctgacttaa tacttttact taccactctt tcaacgtgaa tagttaagta taatttttcc
    41341 tcatctgtta aatcgtagtt gtatttttta tgtataaatt catccaactt ttctatgaat
    41401 ttataagcat ttttatattt aacttttatt acatttaata aatcatcttc attattttca
    41461 tgtaaattat tactaacaat tcgttctgca aaatacttta agtgagtaat aaatctatga
    41521 taataaactg aatcttcatc aaatactatt ctgtaatggt atttaaccat atttgaaatt
    41581 tcttgtatta tttgtgtaat ctcatatata gtttttatac tttgatccat ttgtgcatta
    41641 actatatgca gtgctataaa tccagcttca tcttctgata gtctaatttc aaattttttt
    41701 tctatataat ccaatgcctc taaccctatt ttaaattcac tcttataaaa tctttttata
    41761 tcccaaagta atacattttt tacacttact ccttcacgag ctctttctat tgcagtatac
    41821 atatggtctg ttaaagaaat ataaatacta tcatttaatc ttttccctag tttttcttta
    41881 gcatataaaa ttatttcatc tgataattcc atatatctta taggaatatc tgctattagt
    41941 tctttaaatt tatcagatat atttgggtct gaaagtttga atatcttatc aattttatcc
    42001 tttgatatat gctctcctgt acgtttttta taagctaaac ctctccccat aactactatt
    42061 tccttatgat tttcatcagt tgtaataatt acattattat tgagtatttt ttcaataatc
    42121 attgtgatgc cccctttatt agcattaagt taaaataaat cttttttaat tatttagaat
    42181 atttaaaatg taatttatta cataaaaaaa aacctattta ttacacatta aatagactcc
    42241 catgagtcct gagtaataaa taggtttagc ttgtatttaa acaatcacta tccgtttgta
    42301 ttttatttta attattattt attatatgtt agcaaattta ttttttagtg tcaacttttt
    42361 ctcaatattt taattttaaa taaattatat aaattgaatc ttcaaagtac tatttttatc
    42421 aataaacaaa ataaaaaagg tattactttc tttaagtaat acccatttat ttttaattta
    42481 agtggttttt cttaatctca tcaagtagtc catcttctaa ataaatttct aatgcacatc
    42541 ctaccattgc ctttactgct ttgtgaagtt tatcataagc ttcctcagaa tttgcataat
    42601 ttagaaattc tttttcatga acatatacca ttggacttat atctaaggct atttctgtat
    42661 acatagtagg gcaaacttga cttacattac ctatatctgt agaacctact ggaccatctt
    42721 taccttctaa tatatctgtt atacctgcct caatcaaatt attctttgtt atcttttgaa
    42781 gtactttgtt atttattatg ttgtcaaaag aattttcaaa gtatctataa gaaagcttag
    42841 ctcctgtcat taactctgca ccttttgcac agtttataac ttttttagtt acttcatcta
    42901 aataacttct ttcagcagct cttacgaaaa acttacaagc tgctttttct ggaactatat
    42961 ttggagcctc tcctccattt gaaactatac catgtattct aacatctgat tttacatgtt
    43021 gtcttagtgc atttatccca gcaaaagtaa gttgtactgc atctagtgca ttgactccca
    43081 tatgagggtg actagctgca tgtgctgata ccccttcaaa actaaactct aatgaatcta
    43141 tcgccaaagc cttacagttt atattattaa aagcttctat atgcatttga taacttacat
    43201 caacatcatc aaaagctcct gccttaacta aatcacattt tcctcctgtt gtttcttcag
    43261 ctggtgttcc tattagtaca atttttcctt taaaattttc ttttaactta cttaatacta
    43321 tacaagctcc tagtgtagat gctgcaatcc agttatgtcc acaagcatga gctggttctt
    43381 tattttctcc atatccagga agtgcatcat actcagctaa aaatgctatg gtaggtccat
    43441 cattatctcc taattcagct ctaaaagcag tctccatatt acaatatgga taaactgtat
    43501 caaatccata ttccttcatt ttttctacta agtattttga tgatatgtat tcttcttccc
    43561 ctaattcagg attgttaaat ataaaattac aaacttcttc aatttctttt ccaagttcat
    43621 tctctatctc ataagctctc ttctttatac tctctatcat aatttacctc ctactagaat
    43681 cctagatata atcctattgt caaaaatgcc attacaagaa ttgttaatac taccattaaa
    43741 ggaattacga atctaaccca ctttgaccaa ggaatattgg ctactgctag tgctcccatt
    43801 aaaacacctg atgtaggtgt tatcaagtta gttacaccta taccaaattg ataagctgta
    43861 actatcattt gtcttgatac atctactaag tcgccaagtg gtgcaagtac aggtattgta
    43921 agtgatgcaa gccctgatgc tgatggtatt aaaaatgcta ttaatccttc tattaagaaa
    43981 gttaaattta taaatatagt ttttggtaat ccatttaata aatttgctgc agaatttaaa
    44041 attgtatcta ttataaatcc attttctgct attattacga ttccacgtgc aaatgctata
    44101 cacaatcctg ctgtaattaa atctcctgct ccacttataa atgaattaac tatctcatct
    44161 tgtttaagcc ctgatgctat tcctgatatt atacctatag ctgtaaatat cattgcaatt
    44221 tcagttatgt accattctag tcttaaaact ccatatatca taatcgccat acctattgtg
    44281 aaaatggcta ttaccatggc ttctcttttt gtaaattcct ttatatttgt atcactatta
    44341 accattaatt cttgatttag tgatatatca tgtactaaag attttttagg gtctttttta
    44401 actttattgg catacatcat aacaaatcct atagatatag ccatatatat tataaacata
    44461 acaactctaa actctattcc agaacctggt actatttgtg ataatgcttg tgctattcct
    44521 acagagaaag gattcgtagt agctgctcca aatccagctg ttgctcctat aaatactgtg
    44581 gccactgctg tcaatgaatc atatcccata ctggtcataa gtggaataaa tatcatataa
    44641 aaaggtagtg tctcttcact cattccaaac gtcgtcccac ctagtccaaa tagaatcatt
    44701 gaaatcggta ttattaaaaa ttgaaacttc ttcattttat ttacagtatg agctataaca
    44761 gcttctatag ctcctgtttt attaactatc ccaaaagctc ctccaactat taaaacaaat
    44821 ccaactactt cagctgcatc tataaacccg tttataggtg ctgtaaatac atcagtaatt
    44881 ccttgtggat ttgatggtgc tttttcataa gttccagcta caacaactga ccttccgtct
    44941 atatctactc tttcaaaatt tccacttggc actatccaag taagaattgc cattaacact
    45001 attagcaaaa atattattgt aagtgtctgt ggcatagtaa actttttctt ttttaaactg
    45061 ctcattttta atctccccac tttttaaatt aaatatttta ttattgcatc cctttatcct
    45121 attttgtata gatgtaaaaa agctcaacat ggatacatgt tgagctaaat gtgctaaata
    45181 caacatacct gcatcttccc tttatcttgt ttaagatagc tctccatcaa aattcgggat
    45241 aattttgatg acagtactgt acctattaaa tacagtccca gcactagctt cggacactag
    45301 cacttcggca cttactcctt ttatattgcc tcattggttt ccaacctctt caatattaat
    45361 cttagtaaat gcaacctcta cctcaatctg tttgttagat gaggtaattc ttattaattt
    45421 ttattaattc aaagtaattg tagcataata tgtcgagatt gtaaatattt tcagtataat
    45481 aattcttact ttatgatttc attccatttt tcacaaataa ttttttctcc atataattta
    45541 aatactgtag tatattttaa atacacacaa actttccaca aattttaaat tattataata
    45601 cctttataat aaaaaataaa agctatgtaa acttatcaat ttacacagct ttatatacat
    45661 taatctactc caagtacagc tttaagcttt gatgctattg taataactcg tggaccaaat
    45721 ataacttgaa ctgagttttt ctttttaaca actccacttg cacccaattc atttacccag
    45781 aagccatctt catagaatcc atcaattgaa tttagctcta ccctaagtct agttgcacag
    45841 tttgtaacat tttttatatt attacttcct cctaaacctt ctattataga atgtacaagc
    45901 tttgtatctt tatcatctac ttcctcttct actgcattgt tgtatgctgt tttaccagca
    45961 tctacagatt gtctttctct atattctttc ttactataaa gcttaacttc ttctgaactt
    46021 cttcctggtg tttttatatc aaacttcttg attgcccatg taaatattat ataagtaact
    46081 ataaaaaata ttggtatcaa aaataataat ggtaacgcat ccactttttg tggttgtaat
    46141 aaattaggaa acatatttct aaatgattct cccattatag aaacattaaa catttctgta
    46201 aatacatacg tcaatcctgc cagtggtaca tatgctacaa aatacaataa tggtgcaaca
    46261 aataggaatg tatactctat tggttccgtt acccctacca acatggctgt tactattgct
    46321 ggtatcaata tacttattgc ttttttctta ttagcattat cagccaactt atacatagct
    46381 aaagctgctg ctggtaatat tgcaaaatgt attaataaac gacctgttgt gaagttacga
    46441 gttatataag atagtgtatc tgggtctccc atttgagcta atcttatatt attaacaccc
    46501 tcgtaaattg ctccacctac ttccattgtt ccacctactc ttgtatattc tatagggaat
    46561 gcaattaagt gatgtatacc aaatggtaat aatgctttat ccattgctcc aaaagtaaat
    46621 gttcctaata atccactaga acctatccaa ttaccaagtt tagataatgc caaccctact
    46681 ggtggccata taaagtacat tgctgtaccc aatactattg atgctataaa catcataatc
    46741 attgaaaatc ttggacctga gaaaaaagat aatgcactag gaagttctat gtcaacaaat
    46801 ttgttatgta ctgcactagc tacaaatcca gctattatag ctccaaatat actacaatca
    46861 tatgaaaaga ttcctaagaa agaaccatat aaagaacttc gactatatgc ttctgcctct
    46921 cctaatccag atgccataaa cgcttctgct gtaactgtat ctgaatttat accatttgct
    46981 gctaacaatg tacttataac tgtgtgcatt cctataaata aaacaattcc tcctaatgca
    47041 ccccatcctt tttccttttt agataatcca aaggctatac ctgtaacaaa gaatattggt
    47101 aagtatctca ttaccataaa tcctaaatcg tttattactt taaagaatac atttataaat
    47161 gacccttctt ttattagtcc gctaagccct aatgcagaaa cgttttccat atttgcaaaa
    47221 gctgacccta atcctataaa tactcctgct acaacaagta atataacagg tatcatcatt
    47281 gctccagaaa gtacttgtaa tttatccttc atagtattat ttcctccatt tttattgaaa
    47341 tgccaggcta tataatgcat atatatatac ttctattgct ttgtataatg tatctatact
    47401 tatattttca tttgcctgat gtgaatttgg attatccccc tctattgtgg gtccaaatgc
    47461 aactccattt tttaacactc ttgcataaga tgcagctcct tttgtaaaaa gttcggcttc
    47521 ttttccaaaa ccttgtttat atgcatttga taatttattt attaattcac tatctggttt
    47581 tacatacagt aattttaaat ccttatatat atcaactatt aaattttctt tttttccaat
    47641 ttcattaatt ctattagtta taaactccca acttataccc ttaggatatc taaagtctat
    47701 tttcatattc aattcatttt tctctaagaa gatactcatt atacatattg tagaaactcc
    47761 catatctaca tcttctttat acatccctaa tttttttcca tgattatcat ctgtaaagta
    47821 tgaatttaaa atatccctta ttataacgcc tttatcattt agttttttaa ttttctctaa
    47881 atcttttgct aaactaaatg cacagttata tgacctgtgt ggatttctcg ataaagccct
    47941 ttctcctgta tatcttacta atactttacc ttcatctaat tcttctatct ctgtataata
    48001 agctaatgat tctactaaat ctttcttatc attagtttta aaaacagcct caattttgtc
    48061 acatacaaat ccatcttctt tgtttgactt tatatcaact aaattgtgat ttctaaattt
    48121 atctttatct attttctttc ttagattgaa gtataaaata cctttttctc catttactat
    48181 tgggaagttt ccatctggag aaaatgcaaa ttttggctgt tcattgacac taaagtaatg
    48241 ctccatacat tcccaagtcg tctcttcagc tccacccact atcaatctaa tctttctctt
    48301 cggcttttca ttaagttctc ttaagaaaag taatgcatat aaaatcccta tcaaaggtcc
    48361 tttgttgtca ttaactcctc taccataaag atagttatct ttttgacata ctttaaaagg
    48421 tttactcttc caaagttctt tattatatat tggcaccaca tctatatgat taagcacccc
    48481 tactacttct tctccttctc catattctat atgaattgca tatccatcaa agtcttttac
    48541 aacaaaatct ttgtcttttg ctatttgaat aagcttatcg aaggcatttc ttatttctat
    48601 cccaaatggt tgatttatat ctgttgtact ttcatctcta attgaaggta tttcaactaa
    48661 acttctaata tcatttatga tattttcttg attggatttt aaataatcaa taattagttc
    48721 tttgtttact atcatcttaa ttatttatca cccctttata ttaattttac aaagcatttc
    48781 agttcatatc tacattataa attttgaata ttattttttc aatatttcaa atacttctct
    48841 aagttaccat aatgatacgg taaacaatca acagattatg acaaaatcat actataccta
    48901 ttactaatat tttaacttat ctattacgtc ttttttaatg taagtttatt ccaaaaatac
    48961 ataaaaaaag atgtagcctt agctacatct tcttatatct attcaacttc cattattatg
    49021 ctattaatat ctacatttcc aggttctatc ctatctttta ctgttctctt aacagaatca
    49081 gcatcatcca ttataactac aggagttatt atatttaaat catttgacgt tataaaatct
    49141 aaatctactt ccattattaa atctttagct tttaccttat ctcctgcctt taccttaaca
    49201 tcaaacggct ttccttctag tgacattgtc tctaaaccta catgtattaa tatatttgta
    49261 ttatcattac tcttaatcat tattgcatgc tttgtatcaa atacattgac tatttcacca
    49321 tcaacaggag aatataactt accttcactt ggtattattg ctactccatc tcccatcatc
    49381 ttttgggcaa aaactgcatc tggaacatct tctaagttca ctaaatttcc cttaaaaggg
    49441 ctatatattt tctcaatctt attttctttt ttcttaaata tattaaacat aacacactct
    49501 ccacattata aaattttatg ataatatttt tcttaaagct tttgctacac catcatttac
    49561 attagtatca gttacaaaat ctgccataga ttttacttca tctgtagcat ttcccatagc
    49621 aataccaaga cctgcaaatt taatcatgga tatatcattt tcattatcac ctatacaaat
    49681 aacttctttg ctatctatac ctaatatatc acaaaatttt ttaactgcaa taccttttga
    49741 agtaccttta ctcataactt caaagtttat ctttccagaa cttactactt ctaaatcttc
    49801 ttgtttttta aattcttcct ttgcttctct caaagaatct acgttttctg agaaaagtat
    49861 acattttaaa atttcattat cagctttttc taataaagtt tcacaacttg cattttctat
    49921 tatttcaatt ctgtcttctt ctttattttt actatttatt aatctatatg gatgttcttc
    49981 acctatttca ttgttggata taactatttt acagccttta aaatgtttgt ttaaattata
    50041 cttttcacct attgtataga ttttctttag agcttctttg ctcaaaacct tcttatactc
    50101 atatcccagt aatttaaaat atgtcccatt agttgatata atatatatat catcccctaa
    50161 aacacttgta aaatatttca tagcattata aggtcttccc gtaactaagg ctaccttgat
    50221 acccttttcc atggcttctt ttagtgcttt aatattttct tctgatactt caaatccttt
    50281 acccatcaaa gtaccatcca tatcagtaca aattaattta tatcgcattt gtatcacatc
    50341 ctagtaagat ttttctaata tttcaacaag gtcttctttc tttccaaaag gtaaaaatcc
    50401 taaaactgga cagcttgtta tttgttctaa ttgttccttt gttacattaa actcagacat
    50461 ttttgtctta agccccactc tctttaaaaa actttctatt tcatttccta aagttttagc
    50521 tgcttcatca tcttccactt tttctaattc ttggtttagt attctcgcta ctttggcaaa
    50581 tttctctata ttatccttat agaacttttc tgcaaatttt gggaacacta tagccaaagc
    50641 ctcaccatga ggaatatgtg taactcctcc tattatttca cttagtggat gtggtgctgc
    50701 tgctccagca tttgccagtg aacttcctcc taatgtatct gctactgcta actttgttct
    50761 gtattctata ttatttttat cttccattac tttaggtaga tagttaatga ctaattccat
    50821 agatttttca gacattatct ctgtaaaatc attagcattt ggatttatat aactttcaaa
    50881 tgcatgagta aaagcatcaa atgctgttga tgcagtaatt ctctctggca gagttaacat
    50941 aagctctggg tcaactatac attcttttga gaaacaattt tgatgaaata ttgtattttt
    51001 ctcgcttcct ctcgatatta ctgctgcttg tgtaacttgg cttcctgttc cagaagtagt
    51061 tgatacagct attaatggta attctttttc acttacctta tcgtatattt taaatggatt
    51121 tgtatatgta ctaaacatat aatcccaatc tattttttct aaaccaaaag tcaatgctaa
    51181 tacttttgca gtatctatac ttgaacctcc tccaacagct aaaacaacgt ctacagattc
    51241 ttttttagct aattcaaacc cttcgtttat taattctaca gttgggtttg gctctactct
    51301 ctgaacctcc catgactaaa gtcacagggt tcctgcttca cagaattttg catactaaaa
    51361 tatgtcttac taattctcca caggccatcc ccgtagttcc tacggttctt acataagtta
    51421 tttattttga attgttatta gtcttaatcc ttcttttaat atatttatac ttgcatttat
    51481 atctctatcg tgagtttcat tacaactagg acaaatccat tctcttatat ttaagtcctt
    51541 cacttcctcg tttttatacc cacatttatt gcatatttgt gaacttgcaa agaatttacc
    51601 tacctttaca atttgtctac catgccaatt agccttgtat tctaattgac gaataaattc
    51661 tgaccatgat acatctgaaa ttgagcgaga tagtttacga tttttaatca tattctttac
    51721 ttgtaaatct tctatgcaaa taatatcatt ctcttttatt aatttagtag ataatttttg
//
LOCUS       DAMJYR010000013        49708 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.013, whole genome shotgun sequence.
ACCESSION   DAMJYR010000013 DAMJYR010000000
VERSION     DAMJYR010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 49708)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49708)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49708
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.013"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            <1..117
                     /locus_tag="PC471_000740"
     CDS             <1..117
                     /locus_tag="PC471_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall hydrolase"
                     /protein_id="HDF2787880.1"
                     /translation="IGGAACEKIGSMTKEKFTMIKGNDRFDTLYKALDFINR"
     gene            274..2622
                     /locus_tag="PC471_000741"
     CDS             274..2622
                     /locus_tag="PC471_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HDF2787881.1"
                     /translation="MKNLSKKAVILSLSLVVVSPLVHKINAQDIEKNREVRYETRKAN
                     NLENMTMEEAFPDENFRKVICDKLSILDDGNPIGISQKAIIESTKGLTLGSKSIKNLS
                     GINYFIGLENFSCRGNNLTSLDLSSNINLKELYCNENQLTSLDLSNNRELTTLHCGEN
                     NLTDLVIENSKLDELNCRKNNLKSLDITKATNLTTLLCFENELTSLNLSKNQKLKILK
                     CDYNNLNDLDISTNLELQDLNCSFNQLENINLDNNKELVKLICHNNKLQNLNLKNHEK
                     LVKLYCNQEQLSTLNVENCINLEDLTCSRSNLKNLDISSNKNLKYLECVANKLTNLNI
                     ENNIELLELYCYDNEIKALDISKNIDLENLTCYKNKLDSLNTSKNKNLKYLYCSQNEL
                     TSLDVTKNTELVRLYCGENRLTSLDVSKNTKLKELDWSNQKKSTSTGGSSGSGGGSSS
                     TNEESSEPTSTPSKEKLTGVDRNETSVKISQKGWNKADNIVLINDSSISDALSATPFA
                     KSKDAPILLTKNNNLNKLTEKEINRLEAKNVYIVGGLKSVDEKVVSDLKKKGLNVIRI
                     SGNDRYETSIKLAKELDKNSNLSKVVVVNGEKGLADAVSMGAISAKEEMPILLTNQND
                     DMKDIKDLIANKNISKSYVIGGESLFNNKEVNNTLPSVTKIAGSDNNKEVNNTLPSVT
                     KIAGSDRTETNSKVISHFYSKDTLNDLYVAKNGMNKQDDLVDALSVGVLAGKTESPVV
                     LVGNGLDNSQKELIKNKKFKNITQIGGNGNEKAFNEVENLVK"
     gene            complement(2664..2849)
                     /locus_tag="PC471_000742"
     CDS             complement(2664..2849)
                     /locus_tag="PC471_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HDF2787882.1"
                     /translation="MILLEQRKLRIGFNKSGKGSFTPRLILPMSWIKEMNISPDERDV
                     LVTFEDGKIIIEKTENE"
     gene            complement(3367..3543)
                     /locus_tag="PC471_000743"
     CDS             complement(3367..3543)
                     /locus_tag="PC471_000743"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2787883.1"
                     /translation="MEQKESKIVYSKGGSGTYSAKISLPLSQLEKMGFTREKRKAVVI
                     FKEDEIIIKKSDEE"
     gene            4260..4937
                     /locus_tag="PC471_000744"
     CDS             4260..4937
                     /locus_tag="PC471_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HDF2787884.1"
                     /translation="MFKHDKALLKEVKVERPNPQYAVLMQEQLGGANGELKAAMQYLS
                     QSFRIKDPQIKDLFLDIAAEELSHMEMVAQTINLLNGHDVDYNAVNTGEIETHVLTGL
                     SPVLINSSGAPWTANYVTVTGDLVADLLSNIASEQRAKVVYEYLYRQIDDKYVKETID
                     FLLNREEAHNALFRDALNKVKDTGSNRDFGVTEDSKLYFDLSTPGPNHDTKIDINPPS
                     FEKPLKK"
     gene            5106..7142
                     /locus_tag="PC471_000745"
     CDS             5106..7142
                     /locus_tag="PC471_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HDF2787885.1"
                     /translation="MDSNRKPNNLINEKSPYLLQHAYNPINWYSWNDEAFKKAKEEDK
                     PIFLSVGYSTCHWCHVMEKESFEDEEVAEIMNRNFVAIKVDKEERPDVDSVYMTVCQA
                     MTGSGGWPMTIIMTPDKKPFFAGTYFPKYSRYNRPGVIDLLENVSEKWNTSRDILIKS
                     GDEIIEALKDDFGVKNTEGDLSKEMLSSSVRVFKAIYDEKYGGFGNAPKFPSPQNLMF
                     LMKYYSIEKDKDVLKMVEKTLDGMYRGGLFDHIGFGFSRYSTDKKWLAPHFEKMLYDN
                     AMLTIAFLDAYKITKKELYKEIAIKTIDYVVREMKDKEGGFYSAQDADSEGEEGKFYT
                     FNPLEIIEVLGEEDGIFFNNYFDITSSGNFEGKSIPNLIKNKEYERHNEKIADLSKKV
                     FEYRKERTSLHKDDKILTSWNALMIVALTKAYSTLKNDIYLEYSNKCLNFINNNLVNE
                     SGRLLARYRDGSSDYLAYLDDYAFLIWAYIELYESTFNMKYLEKALNLNESCINLFWD
                     YEKSGFYIYGKDSENLIARPKDLYDGAIPSGNSVQLYNLIRLAKITGDNRLEEMSYKQ
                     LKLYVDNVKSSPAGYSFYMLSLMFELYSTKEIICIFKEDSDLIAFKELISENFIPNAT
                     FLAKKYNEENTIIGFLNNYRLKDDKISYYVCQSNSCSQPINDLQKLKDMILGVE"
     gene            complement(7333..7899)
                     /locus_tag="PC471_000746"
     CDS             complement(7333..7899)
                     /locus_tag="PC471_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDF2787886.1"
                     /translation="MDYSNELKELFLMNQTYATLFTLTNKIQIEGDKYFGILTSRQYM
                     TILSILHLPEEETTLNNIARKMGTSKQNINRLVANLEKNGYVDVIPSPHDKRAINVKV
                     TDLGKKVMVTCSRTGINFMADVFHEFTKDELETLWSLLKKMYRFNGEEQDGFEEDANF
                     MEYEEIDKIKSEALEEFAKRRKRVNKND"
     gene            8187..9122
                     /locus_tag="PC471_000747"
     CDS             8187..9122
                     /locus_tag="PC471_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDF2787887.1"
                     /translation="MKCTNFTFKGEEGLDIYTYKWEDENIKKPKAVIQIAHGMAETAQ
                     RYETFAKVLTKNGYIVYINDHRGHGKTAKIIENVGHLAEKEGFRCLVEDMYTLTNIIK
                     KENEDLPIYLFGHSMGSFASQRYIMDYSNNLSGLILCGSNGKQGIILNLAHLIINREI
                     KKYGRRSKSNKINNLIFGGEIIRRNEKTKFDWLSRDKEQVEKYINDPFCGVVCSCGFF
                     YDLVQGLKEIEDKENLKKVPLDIPIYIISGDKDPIGKNGKGVLRLRDRYIKLGVKDVT
                     CKLYKDGRHELLNEINREEVFEDIICWLNNKIEIL"
     gene            9587..10801
                     /locus_tag="PC471_000748"
     CDS             9587..10801
                     /locus_tag="PC471_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2787888.1"
                     /translation="MQIKIGKVIQCLRKERNLTQEQLAKFIGVSTPAVSKWESGNSYP
                     DIELLPLLADFFNVSIDKLLNYKIDLSEEEVMKIYKELESGFARIEIDLSTEEPKEEF
                     RQDLESVKKLSNMYIEKYPKSYLLKLRICSLYQMYSYKFGKSELNDRVKETTNILEDI
                     VRNTDDIQIKETALIILSNAYCMLEDYEKAELYLNMIHKSIGDTSVNLAMIYLKQNRL
                     EEAEILLQNKLFSNVFNISMDCKGIINVYKNQYKELKKKLENRNFNKNAIEREMEYIK
                     NKLLGYANLSLEIKKMLSEDKGAFFSMYMDYMELSLIFLFFNMKEEAKKALYSLKEIL
                     EKYPIHESLDVSQMRFFDKVESKNLYTFNIYTNLLIVLNDDSYNELREEPIFKDVIEK
                     ILDMEKMLKNKE"
     gene            complement(11221..11742)
                     /locus_tag="PC471_000749"
     CDS             complement(11221..11742)
                     /locus_tag="PC471_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDF2787889.1"
                     /translation="MKFERLRTSENKLYYKAMELYKISFPFHKQRKSSLQAEILKNKE
                     YQFNLIYDKNQFVGIILCWETDNFIYVEHFCIFPEIRNNRYGQRALELLNKKGKTIIL
                     EIDLPIDEISIYRKAFYERVKYKTNNYEHIHPPYHKKFNGHNLIVMSYPEKLSKMEYD
                     KFNHYLRNNVMSF"
     gene            12098..12895
                     /locus_tag="PC471_000750"
     CDS             12098..12895
                     /locus_tag="PC471_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HDF2787890.1"
                     /translation="MEKREKRIIISSLLSVSILMGLASIYSILNKEDISLTVKGQEQK
                     VSSFKKTVEELLDEQGVKYNSEDKINPSLDTELKDDMKIKVVKVTKSKKEEIEKIPFD
                     TKHVNDSNLLKGKSKVYQEGQEGEKKLVYNLTYHDGKLVKKVLSKEVISKEPTTKIIK
                     YGTKEKVLIASRGANIRGGKHMKVVATAYAGDTITSTGTTPRWGVIAVDPRVIPYGTK
                     VYIPKLGMTFVAEDCGGAIKGNRIDIFMNSEGKASNWGRKSIDIYLH"
     gene            13005..13595
                     /locus_tag="PC471_000751"
     CDS             13005..13595
                     /locus_tag="PC471_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HDF2787891.1"
                     /translation="MKIVCLGDSLTYGFGVSRSNSWTNIVNKETRLEIVNKGINGDTT
                     SGMLARFNEDVVKNSPDIVFIMGGTNDFIAGAGNEIINSNIMAMVHQSYSKNIIPIIG
                     IPLKPDIPNVREDWSCFTDFNIVSQNLESYSYWIKKFCMTFNTNFVDFYSEYNKNMEL
                     EGYKKLYFDGLHPTKEGHRIMADIFINSINKYIEEL"
     gene            13661..15358
                     /locus_tag="PC471_000752"
     CDS             13661..15358
                     /locus_tag="PC471_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HDF2787892.1"
                     /translation="MDYTKTYEEWIKGSYFDEDTKLELENIKNNEKEIEDRFYKDLEF
                     GTAGLRGIIEAGTNRINKYTVRRATFGLANYILENTTKEETSRGVVIAHDNRHKSRQF
                     CIESANTLAACGIKAYIFDSLRTTPELSFAVRSLNAIAGIVITASHNPPEYNGYKVYW
                     EDGAQVMPEIANAITEKVNSIHDYSTIPTLTEANKNLVVLLDESQDTKFIEAVKSQII
                     RKDLVKNVGKSFKIVYTPLCGTGNVPIRRALKEVGFENIIVVPEEENPDPNFAGLDYP
                     NPEEKKALNRGILLAKEKGADLVIATDPDCDRVGVAVKTTTGEYALLTGNQIGGMLTH
                     YIIEGLKENNKLKENPTIIKTIVTSEFGADIAKANNVDVLNVLTGFKFIGEKIKLFEQ
                     NKNRSYVFGYEESYGYLVGTHARDKDGVVSSLLISEMAAFYYSKGMSLYDGLIELYKK
                     YGFFKEQTISLTLKGIEGVEKIKEIISYFRENQIDYINSIKVVDKKDYKNGIDNLPKS
                     DVLKYFLEDESWVAIRPSGTEPKLKFYIAVKGASDIEADKKIQGLKKYIDDMVEKLK"
     gene            15572..16411
                     /locus_tag="PC471_000753"
     CDS             15572..16411
                     /locus_tag="PC471_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HDF2787893.1"
                     /translation="MFGIDKKLIEYIIAKSNYSELSSKSAEVSFFLMLSIFPFLIFTI
                     SSIAYIPILHLNKYIALFRNMMPEGAFAVLSSIIVSAIDNRNLKFLAVSFVLTMWTFS
                     RAVKALIKGMNRAYKVKETRSFFKILSISFLFTIMLLVLIFLSMIFLVYGEKIGYFIF
                     NLVGLDEIFIKIWDILRYTVGIITIIVIFTLLYKYTPNKKLTIKESAPGAIFATFAWF
                     LVSFLYSYYTNYYANYEVIYGSIAEIIVLMTWMYFSSWSIVIGYEVNSRLYFRKIRHE
                     MLK"
     gene            16928..18784
                     /locus_tag="PC471_000754"
     CDS             16928..18784
                     /locus_tag="PC471_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HDF2787894.1"
                     /translation="MDTHNKYVNFIKNIPVPFLYCRIVKRQEDIEYRVEYISKGMGKV
                     LQLEEGICDKNILDVLPVFKSKKYFKELFSNEVDCIKRYIPTLKNWINIKKQIIGDSY
                     IILYFGKIVFDYRQIIDSFDKKEKVAYIKDEEGTYIDCSENLIPILNNNIKTTKDIFG
                     KNDIEVWGENTGKLFRDDYREGVSSKKRFLQNLFEYEETFFMVEKYFLYDEDELLGTI
                     GIVDNIIYSGYSNRNYNSKDLMKMIEHSIPENMFYKDVYGNYIGFNSGFLNLACMNKE
                     ELLGKNSYKISEEEALIDKIFESDKGVVENKKVVTFELNISMNDENKCIEITKRPFFD
                     SYGSVIGIIGTARDISRRKRLEEEMDKTRMEFFANLSHELRTPINLISSSLQVIEKKE
                     ADLIESNDTLKRNLGIIKQNGNRILRLVNNVIDFTKMQSGYLDFKPEESDIIAFIEEI
                     CMSVADFASQNNIQLTFDTEIEEFSMLFDSEKLERIILNLLSNGIKYNKKDGKINIFL
                     YVKDNVFNMKISDSGIGIPKEKIDKIFNRFEQIDNELSYRVKGSGIGLSLVKSLVELH
                     EGSISLKSQLGIGSEFIVSLPVRSKNNIEKYNHKREISNELSKKLEIEFSDL"
     misc_binding    18867..19107
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     misc_binding    19173..19408
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            19493..20710
                     /locus_tag="PC471_000755"
     CDS             19493..20710
                     /locus_tag="PC471_000755"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HDF2787895.1"
                     /translation="MENKVKIGVLGYGVVGSGLIDIIDNNKEKRNIEIVGILVNNLEK
                     HKDKKYSNIITNNIDDIFNKDIDILVEVMGGLEPSLSYIKKALNNKIHVVTANKDLLA
                     ECGDELAKLASENKVSIKFEASVAGGIPVLKPIIESLEGNNIDSINAILNGTTNFILS
                     KMYDENLSYDMALRQAQELGFAEANPESDVLGYDAARKLSILSTLAYDNRVYWKDVYL
                     EGITDIDEKDIEYAKKLNCKIKLIGQSKYENDKVSAFVRPVLVEKDNILARIDNEFNA
                     VIVNGDSVGEVSFVGKGAGSLATGSAVYSDVIDIIDNRVSSIDSFTKDKIQVNKIVRE
                     KCGALLRFKKCNKDEILNIVENCLVKFDILNDDDELAIMVYADSEYEINNSLCLIKDK
                     GYCEKMNKMLKIS"
     gene            20827..21309
                     /gene="cdeM"
                     /locus_tag="PC471_000756"
     CDS             20827..21309
                     /gene="cdeM"
                     /locus_tag="PC471_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exosporium morphogenetic protein CdeM"
                     /protein_id="HDF2787896.1"
                     /translation="MENKKCYSEDWYERGESTAKWFQNDREEYEREAYDEDRERRGSN
                     CGCSDSGENRPRNCERFRREAEIREREAREAFCESSEKKKEALAYECEARKLWEEAEK
                     YWDEYSKYNYKGIEYLAEAARLFDEGMECEARRNGNNGGNNNNCCHKCHKCNCNCCRK
                     "
     gene            21573..22838
                     /gene="hisD"
                     /locus_tag="PC471_000757"
     CDS             21573..22838
                     /gene="hisD"
                     /locus_tag="PC471_000757"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HDF2787897.1"
                     /translation="MKSIKTSSMKEKVDEKILREQVSSIIEDIRNNKDVALKKYNEKF
                     DRNTRDEFRITKEEIKEAYKHVDDEFINNLKIAAKNIREFAKAQKLSFENPFEKEIYP
                     GVILGQTNIPIESCLAYVPGGQYPLFSTALMLIIPAKVAGVKRIVACSPTMKNTEKIN
                     PKTLVAMDIAGADEIYATGGVQAIAAFTYGTEKINPVDIIVGPGNKFVTEAKRQCYGQ
                     VGIDFVAGPSEVLIIADETSNPVYIAADLLAQCEHDLNARGILLTNSLEVAQKVEKNI
                     ESMLKDLPTKDIAYSSWENNGEIILVDDMEEAIKISNFYAPEHLEVAVNEYNDICDRL
                     TNYGSLFIGNLSAEVFGDYVSGTNHTLPTLKASRYTGGVWVGTFIKTCTKQIFNEKAI
                     QSLAPVAEKLAKEEGLYAHAKAAEVRFKK"
     gene            complement(23041..23793)
                     /locus_tag="PC471_000758"
     CDS             complement(23041..23793)
                     /locus_tag="PC471_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDF2787898.1"
                     /translation="MSYFQYQGNSCFYKEYGQGKPIIFLHGNTGSSNMFKALVPLYAE
                     NFRCILIDFLGNGQSDRVSQFSPDIWHDEALQTIALIEHLNCGKVGLIGTSGGAWAAV
                     NTGLERPDLVEAIIADSFDGRTLNDNFIDNLITGREKSKQDIQARKFYEWCHGKDWEN
                     VVNLDTKALLQCANEKRPLFHKELCKLEMPILFTGSKEDEMCRHNLEEEYKQMATFIS
                     KASIYIFSQGGHPAILTNADEFAQLAKVFFKY"
     gene            complement(23911..24180)
                     /locus_tag="PC471_000759"
     CDS             complement(23911..24180)
                     /locus_tag="PC471_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2787899.1"
                     /translation="MSYQNSIDLLPKELIEQVQEYIDGKVIYIPKKQEHKKHWGENTN
                     TKQVLASRNSQICINFQNGMSIKQLSEKYFLTEKSIQRILKQQNL"
     gene            24655..25749
                     /locus_tag="PC471_000760"
     CDS             24655..25749
                     /locus_tag="PC471_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HDF2787900.1"
                     /translation="MKNIVGKPQTIKKVNEDLIKNIIKNEGPITKPEISSITELSLAT
                     VNKIVEQLALKNEVKVSGLSESTGGRRAQLFEINANLEHIIALYYYRNCCIGVVANLL
                     GEIIYQEEFNIRMDTYENVNQDTFFVIDTLIKKEKGNNIRAIGIGVPGVVKKGVVKNI
                     PTIKSWEGINLENLIKSKYNTKVFIENDTNITTIGIYQKQYKDKYKNMALMYLEDGIG
                     SGIVINGELYLGSTNFAGELSYLNIEFGKYNSNNLNLEDYIMSLRKRYLESKDNKFKK
                     EILSIISNFIRTLVCVLNPQAMIIQCSILTKSDMEFIEKDVSNNVGEENFPKLIKVDS
                     LREGSINGVISMCLKETDYQYSLSNKKGGY"
     gene            25751..26701
                     /locus_tag="PC471_000761"
     CDS             25751..26701
                     /locus_tag="PC471_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HDF2787901.1"
                     /translation="MKYLVSIDGGGTKTKFCVSDLDGNILKEHTTGSTNYKSVGIKKT
                     YENINNGFKKILKDLYIDYDDIEYTVFGISGCDSPNDYKIIMDEILKIGINKEKIYLA
                     NDAVLAFYAQADSPGLVIVAGTGSIILGIKEDGEIYRVGGWGYNFSDLGSGYDIGRKL
                     LKKVLLYCDECHEYSDLFSCVLDFFGANSFEQLAYMITDINDNVEIANLASLVIDCAE
                     QGDKLAIEILRESSTELSKLAQVILRKISNPMKMDKSEINIVLSGGTLNKTVYAQMLM
                     NNLKTENTDKNLKFISQENQPVYGGIRLAMALANRRVKHG"
     gene            26694..27878
                     /locus_tag="PC471_000762"
     CDS             26694..27878
                     /locus_tag="PC471_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2787902.1"
                     /translation="MDRKKVIVQIFTGGFKAKEIKFNDIKCKLLELLDCIDIEKVIMG
                     WSVDEELYVETKKLLNKYNVELYLWLPCFSEIGLLEKSNLLIDYMGKEVKGYALQEDE
                     NFEFYCPNNKENIKNIKKIYSTYFSDIKFDGIFLDKIRYGSFSNKLNGVFNCFCKDCL
                     NIYKKNNIDKESLMREMNNVSKGVIDNEDTPFGITEYINGKYKFKNKIWEDFFETKNQ
                     NIYKALLEISSYFKSKGLKIGIDAFSPFISYFTGQDLEKLQEVADFIKPMMYRTTKAP
                     AGLPFEFERFIDESIHKNKEIGKRNFLDILGIKEYEGDVYPIEFVLEEIDFMTRNLNK
                     DIYVGVEINKKEIISPVTPKYISENISNINKTTVKGYVLSWDLLSAPKENIDEIIKWF
                     RN"
     gene            27891..29387
                     /locus_tag="PC471_000763"
     CDS             27891..29387
                     /locus_tag="PC471_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HDF2787903.1"
                     /translation="MSKILEIQQIYKSFGKVDVLKGINFELEKGEVRALLGANGAGKS
                     TLIKILGGVHSQTKGKVILTGNEIMFKNPHHSKENGISIIYQELSLIPTLTVLENIFL
                     GREILKGSVFLNKKDMLEEYMKICEDFDFNIDPHIVVSNLSVANQQMVEIMKAVSCNS
                     EIIIMDEPTTSLTNNEKDGLFKIISRLKSKGKTIVYISHILEEVFKVCDSASIMKNGI
                     MIGTYDVEDLTKLKITQLMTGNDNHKLIEKKDYTFAKYEEPPVLEVKNISWSNKIKNV
                     SFKVYRGEVVGLAGLVGSRRTEIINLIFGIDNKDSGDIFINGKKVDITSPKVAIKNKI
                     GLIPEDRKNLGLILEQAIYTNSSSVQIDKFKKFLFIDRNKEIEFSKKGVKELGIKVSD
                     VKQEVKELSGGNQQKVVVSKWLEKDLDLIIYDEPTKGIDIAAKEDIFNTIKEFSSKGI
                     GVIFISSDLEEVIRVSDRILVTREGSIVDDLKNENIQVQDIMNSIFNV"
     gene            29410..30360
                     /locus_tag="PC471_000764"
     CDS             29410..30360
                     /locus_tag="PC471_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2787904.1"
                     /translation="MKKAFEKLNLRDYGVLLGFLALCIAISIATPSFLGKQNILNLLR
                     QSSIIGIISAGMTFVIISGNFDISVGAVAALAGAITMKFATGGTNLFASMFLGVAICA
                     VIGLINGIVVAKINVPSLIATMAMVTIVRGLLLMLTGGYPITENIPILDYIGNGYLFN
                     IPIPVVIFFIVVLASFIVLNKTKFGRYVYSVGGNQEASKLNGINVDSHKVKVFIINAV
                     LAGIAGIVLTGRLGTATAIAGEGYDMDAIASVVIGGTSVAGGSGSVLKTVIGVLLMSV
                     INNSFNLLGIDVYFQYIFKGLIILAAVGFDSYSKKKLASR"
     gene            30381..31343
                     /locus_tag="PC471_000765"
     CDS             30381..31343
                     /locus_tag="PC471_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HDF2787905.1"
                     /translation="MKKLMKHLALLLSLVMIFGLVGCSNGGDKEKNGEKKIAVLLPGS
                     TGYFVATKQGIDAKSKELGVSVEYADAQWDASKQLSQAEDFMAKGVDMIALCGVDSAV
                     SERIVKAANDSDVPIVAFTNSIGSNPTGEFKGLVTYIGQNEEETGALTGKIAKNLLGE
                     TGGKAVLIEGVPGTTPQVLRKKGLEKELKDSNIEIVYNQTSNWEKEQALKVTEDLIQK
                     KTDFNVIICQDDNSATGAGQALKDAGLKDKVKVIGLGGSKDGLKAITDGLIDGTTYMS
                     AVEEGGLVIEKASKFLKGEKIEPVTQIKQVEVNKDNISEFKGEW"
     gene            31715..32347
                     /locus_tag="PC471_000766"
     CDS             31715..32347
                     /locus_tag="PC471_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HDF2787906.1"
                     /translation="MARRLFLERIGLIFLGSAILAFGVYNFYYLNNITEGGVLGILLL
                     LKNLFNIQPAIANVVIDGLLLLVGYKFFGKKFLIYSIVASITFSVLYDLFEAIGPLVP
                     QSQNMLLSTILAGVTVGTGVGIVVKAGCASGGDDALALVISKTTSLNIGQVYLATDVI
                     VLLLSLFYLSAFDIFYSLIAVTISGKVIDFIYYHGKSLDMDISNDIVPEC"
     gene            32758..32850
                     /gene="kdpF"
                     /locus_tag="PC471_000767"
     CDS             32758..32850
                     /gene="kdpF"
                     /locus_tag="PC471_000767"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR02115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HDF2787907.1"
                     /translation="MEGLPMWFLAVVIIMLIIYLVYALINPEKF"
     gene            32891..34573
                     /gene="kdpA"
                     /locus_tag="PC471_000768"
     CDS             32891..34573
                     /gene="kdpA"
                     /locus_tag="PC471_000768"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015391009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HDF2787908.1"
                     /translation="MFMVQIVIVLAIFMILVIPMGKYLYHIATNQKTFGDRLFDKVDN
                     FIYKVCSIDKKKEMNWKQYALALLFTNAVMVFIGYIILRTQSMHIFNPSGIKSMEQGL
                     SFNTIISFMTNTNLQHYSGESGLSYFSQMTVIIYMMFTSAATGYAAAMAFVRGLVGKK
                     KTLGNFYVDLIRITTRVLLPGALIIGLILVTQGVPQTFAGTETVTTIEGKLQDIARGP
                     VAALESIKHLGTNGGGFFGSNSSHPFENPTIISNIVEILSMMILPGACVVAFGHMIKN
                     KKQGWVVFGAMSIIFLIGLVVCFKAESAGNPILSQLGLNQSMGSMEGKEVRFGIAQSS
                     LFTTVTTSFTTGTVNNMHDTLTPLGGLVPLLNMMLNVVFGGKGVGLMNMLMYAIIAVF
                     LCGLMVGRTPEFLTKKIEGKEMKLIALLIILHPLLILMFSGLSVAIPAGLEGISNPGF
                     HGLSQVLYEFASSAANNGSGFEGLGDNTMFWNITTGVVMFFGRYVSIIVLLAISSLLA
                     SKKAVNESIGTLRTDNFTFTIVLVLVVLIVGALTFFPALALGPISEHLVLWH"
     gene            34598..36655
                     /gene="kdpB"
                     /locus_tag="PC471_000769"
     CDS             34598..36655
                     /gene="kdpB"
                     /locus_tag="PC471_000769"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010966942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HDF2787909.1"
                     /translation="METKKTKFITKDIFKTSIIEAFKKLNPKYMMKNPVMFVVEIGFI
                     VTLILTIVPSLFGDEGNNLRAYNGIVTIILFITVLFANFAEAVAEGRGKAQADTLKKT
                     RKDTVARVIDTLGNEKMISASELKLGDIVLVNAGEVIPNDGEVIEGIASVDESAITGE
                     SAPVMRESGGDFASVTGGTTVVSDWLKIRITTKPGESFLDKMIALVEGASRQKTPNEI
                     ALNTLLVGLTLIFMVVLISLYPMANYAGVKIPISTMIALLVCLIPTTIGGLLSAIGIA
                     GMDRVTRFNVIAMSGKAVEACGDVDTMILDKTGTITYGNRLASEFIAVGNADSKDLIN
                     YSVMCSLKDDTPEGKSIVDLGMKLGSTGKTKEAEEAEFVEFSAQTRMSGINLADGRAI
                     RKGAYDSIIKRVKESGGTVPEDLEENVNRVAKLGGTPLVVCVDNEIYGIIYLKDTVKS
                     GLVERFARLREIGIKTIMCTGDNPLTAATIAREAGVDGFIAECKPEDKIDAIKKEQLE
                     GKIVAMTGDGTNDAPALAQADVGLAMNSGTTSAKEAANMVDLDSDPTKILEVVEIGKQ
                     LLITRGALTTFSIANDVAKYFAIIPAIFTLAIPQMEILNVMKLSTSFSAILSALIFNA
                     IIIPCLIPLAMKGVKYRPMKSQAMLMRNMLIFGLGGVIVPFLGIKLIDLLITPLVMVL
                     NLG"
     gene            36674..37300
                     /locus_tag="PC471_000770"
     CDS             36674..37300
                     /locus_tag="PC471_000770"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
                     /protein_id="HDF2787910.1"
                     /translation="MKTLKPALLVSIVLLVVCGLVYPLVLTGVSQVAFKDKANGSMIE
                     VNGVKVGSELIGQSFTDARFFKGRVSSVNYNTYTKEDLVPDKDGNTSYGGVSSGSFNY
                     GATNPELHDRVQKDIEKFLKDNPTVKREDIPTDLLTASGSGLDPNISPASAKIQIPAI
                     AKASGISESKLQKIVDDNTSKKLFGVLGEDRVNVLKVNVEVAKILGLI"
     gene            37537..38445
                     /gene="cysK"
                     /locus_tag="PC471_000771"
     CDS             37537..38445
                     /gene="cysK"
                     /locus_tag="PC471_000771"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HDF2787911.1"
                     /translation="MLYNNALELIGNTPVVRLNNLGCTNGCSNIYVKLEKVNPAGSIK
                     DRAVYEMLEGLEQRGELKKGDVLVEATSGNTGIALSMIGKLKGYEVVIVMPETMSVER
                     RDLMKAYGANLVLTDGKAGMAGSIEKANELLKENPNYKSLKQFENKDNPNAHYKTTAV
                     EIMNDVKDLDIFVCGVGTGGTLIGTARYLKEQNPNIRVIALEPKKSPAISENKTGPHK
                     IQGIGANFVPENYDSSVVDEVILVDDEDAFDTVKLLAAKEGILVGISSGANIFGAIEM
                     AKKYPDKKIVTVAPDGVDKYMSMGIF"
     gene            38472..39062
                     /gene="cysE"
                     /locus_tag="PC471_000772"
     CDS             38472..39062
                     /gene="cysE"
                     /locus_tag="PC471_000772"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HDF2787912.1"
                     /translation="MFKKINKDIEYIMKNDPAARSKIEVFLLYPSVHAMIMHRMAHAL
                     YKKKKLFTARLISQISRFMTGIEIHPGAKMGEGILIDHGMGVVIGETAEVGNRVTIYQ
                     GATLGATGKDTGKRHPTVGDDVLIGAGTKILGPLNIGSNSKIGANSVVVKDVPNGATV
                     VGIPAKIVKIRNLEPVKKNKKEVSYEYDELDNVYYI"
     gene            39222..39410
                     /locus_tag="PC471_000773"
     CDS             39222..39410
                     /locus_tag="PC471_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HDF2787913.1"
                     /translation="MKANVNQDTCIGCGLCPSICPEVFDMKDDGKSHVIVDEVPSDAE
                     ESAAEARESCPVDAIDVH"
     gene            complement(39464..40333)
                     /locus_tag="PC471_000774"
     CDS             complement(39464..40333)
                     /locus_tag="PC471_000774"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl aminopeptidase"
                     /protein_id="HDF2787914.1"
                     /translation="MKLNRNDSCWCGSTKKYKKCHMDMDNKIDSFAKLGHIVPTHDII
                     KNESQIEEIKKSAVINTKILDYISDKIEIGMSTEDINKLVHDFTLQNGAIPAPLDYNG
                     FPKSVCTSVNNEVCHGIPSKDIILKDGDIINVDVSTIFNGYYSDASRMFMIGNVNENA
                     KKLVQVTKECVQKGLEVAKPWGFLGDIGDAINSYAKKNGYSVVREIGGHGVGLSFHED
                     PFVSYVSKKGTEMLLVPGMIFTIEPMVNEGTHEIFVDEENNWTIYTKDGKLSAQWECM
                     VLVTETGVEILTE"
     gene            complement(40530..41774)
                     /locus_tag="PC471_000775"
     CDS             complement(40530..41774)
                     /locus_tag="PC471_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2787915.1"
                     /translation="MENNLNTAKRNNTELLQNDSLKVNLQIGVDFTLLSKSLKAFYKK
                     EKDFYCIALAPCNVSSEKANRKISIYDMIKEINILIESITDKNELLSEELFTNNLSEF
                     YDESLDEIYIDLKQAYLYIKKNTDGLNIEDSIEYVFDVDISNDIKAKENPLINFNSLS
                     LVVWSGETKHILQQMNILDLEDIHSYSIENNTNITIYENNTLQYKFDEESENIKDSSI
                     DIIEQMLILPDDLNYDKDEVENMKNRLAKINIKYLQTLKEKDIKIKLINSNLTDEPEF
                     SDLKYQLPPCWVRSGKTWKDVPGIYRNNSIVAKIGYSNPSYANVHSSKNLELHETAHA
                     IDKNVLNKKSNSEEFMEVFAQERYKLYDPKQVAHAYISKFIEEFFAESFVHYYLDEDS
                     KNTLKENCPLTYDFLEKLELNY"
     gene            42065..42376
                     /locus_tag="PC471_000776"
     CDS             42065..42376
                     /locus_tag="PC471_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HDF2787916.1"
                     /translation="MYFTYIIRCKDDSLYTGYTSNIVRRMNEHKLGINSKYTRAKGFE
                     KLEVYFVTNTKSNAMKLEYYIKKLTRNKKLSIIKNPSILINLIDNKEDYIIGKEIEQL
                     T"
     regulatory      42469..42561
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            42661..43455
                     /gene="thiD"
                     /locus_tag="PC471_000777"
     CDS             42661..43455
                     /gene="thiD"
                     /locus_tag="PC471_000777"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HDF2787917.1"
                     /translation="MSNYKIPTLTIAGSDSSGGAGIQADLKTFSAIGTYGMSVITAIT
                     AQNTKGVFAVEDLNKKIIKKQIEAVFEDIPPRAVKIGMVSSPEIILEIVENLKKYNPK
                     YLVVDPVMISKSGYYLLKPEAKENLIKYLIPLAYIITPNIPEAEEITGIKIHNVDDMK
                     RVGEEILQLGPKFVLMKGGHLDGEAVDILVGKNIFKVYKSERIDKKNTHGTGCTLSSA
                     ITSYLALGYEITEAVNLSKIYITEAIKRSFDIGHGVGPVHHFYKFE"
     gene            43479..44267
                     /gene="thiM"
                     /locus_tag="PC471_000778"
     CDS             43479..44267
                     /gene="thiM"
                     /locus_tag="PC471_000778"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HDF2787918.1"
                     /translation="MYNLIKDVKKLNPLVIHYTNNVTINDCANVTLAVGASPLMSFSY
                     EEVEEMVSVANSVVINIGTMNSNMLDLFLLAGKAANKYNKPVVLDPVGVFASKARAEL
                     TSRLLNEVKFSVVKGNVSEIKFIGGFNVRGKGVDSFDEEEDSTEIIRKIAEKLECVVV
                     ATGKIDIITNGKGTYKINNGTDKLKGITGTGCMTASLIASFMAVTENILEAATMGVLT
                     MSLSGELANLNNPPIGTFKENLMNAIYQMDIDALSKNSNIEFLN"
     gene            44290..44922
                     /gene="thiE"
                     /locus_tag="PC471_000779"
     CDS             44290..44922
                     /gene="thiE"
                     /locus_tag="PC471_000779"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HDF2787919.1"
                     /translation="MIDKESLKKCLKLYLVTDSEMLKGRDFYKCLEDAISSGITTVQL
                     REKNASGREFLRKAMKLREITKRYGVKFIINDRVDIALICDADGVHVGQSDIDVREVR
                     KLIGNNKILGVSARTLEEAICAKNDGADYLGVGSIFTTSTKLDAKSASFETVKEIKEK
                     VDMPFVLIGGINLDNIDKLKCLESDGYAIISAILKAEDISKEVEKWTLKI"
     gene            45061..45363
                     /locus_tag="PC471_000780"
     CDS             45061..45363
                     /locus_tag="PC471_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDF2787920.1"
                     /translation="MIRILLVCVGGMSSTLLVNKMEKDAKKRNIDCKIWAVGEGDIKS
                     ELDNFDILLLGPQLRFMLDDVKSIVGDRAPVSIIDMVNYGTCNGHAVLNSVLEILK"
     gene            complement(45429..46184)
                     /locus_tag="PC471_000781"
     CDS             complement(45429..46184)
                     /locus_tag="PC471_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2787921.1"
                     /translation="MGMLNILWRSMKWRFKNPISFVVTILQPFLWLVLYSSIANQTMN
                     NININNYTAFILPGIIVLVVFSSCSSGGIINFIMKNSGSFYRVLIAPISRYSIVLGQL
                     LEAILVSFIEVTILCIVSIFFSVRIESGIGGILLMIVLIFMTAFFLSSLAYSISLLLP
                     NEIVYETIMTAIVLPIFFLSSALFPIESLSGGLKVAVMLNPFTHVINALRSLIFGETI
                     LIGDIMPVILLFLILCCSSFSLAMWRLKKEMVS"
     gene            complement(46172..47134)
                     /locus_tag="PC471_000782"
     CDS             complement(46172..47134)
                     /locus_tag="PC471_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2787922.1"
                     /translation="MLAIEIDNLVKEYKNGVKALNGLSFNVNAGEIFSLLGPNGAGKS
                     SLINILTTFYKPTSGNVTMFGKDLVDNPSWIRTQIACVAQQISIDEHLSLMENMVFQS
                     KMYKVEPQIAKQRIDSLIDKFDLSSYLKYPTSSYSGGVKRRLDIAMNMVSSPKILFLD
                     EPTVGMDVDSRKSMWDMLLKIRDEYGTTIFLTTHYLEEAEQLSDNICIMKNGKDLAQG
                     TPSSLRSYIRQNILRITFHNTEDIKKYKDSIKSTGLVKFMSVRENSIFISVNDSRTAF
                     TLINKWLLEHDIEFDAIEIVEPSLEDVFLALTSSKKSLKEEWEC"
     gene            complement(47138..47596)
                     /locus_tag="PC471_000783"
     CDS             complement(47138..47596)
                     /locus_tag="PC471_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HDF2787923.1"
                     /translation="MNCKIEIKNIESVTVATMRYNGPMTEATKYFPNVFKAIKGKSNG
                     APFFCYYDVDQKTGVAEMELCVPTAETPNRMGITTKEFPKTKALFFTHTGSYSNLPKT
                     YEMIFKYIHENNIKIQTPWREVFIKGPGMLIKGNPDNYITEIIFPLKEEE"
     gene            47952..48308
                     /locus_tag="PC471_000784"
     CDS             47952..48308
                     /locus_tag="PC471_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDF2787924.1"
                     /translation="MNFELDNTTPIYLQIVKYIKRQIVTGELKPGETIPSRREMALNL
                     KVNLNTVQRAYKEMGDMNIINTFKNYQSSVTVDENILKNLKLELINESLSVFIEDMKA
                     INVSKEEVLKIIEDKY"
     gene            48380..49096
                     /locus_tag="PC471_000785"
     CDS             48380..49096
                     /locus_tag="PC471_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2787925.1"
                     /translation="MIEIKNVSKTYKRMQGLKIRKIEALKNVSFNIEKGKITALLGIN
                     GVGKSTMLKAIAGLINIDSGEIRIDGEKINEKVYNKLAFVPDVQSHFSNTTIKETFEF
                     MEIFYSKWNKEKSKEMMDIFKLDEDEIIDNLSKGNIARVKLILGFCQDPEYILLDEPF
                     TGIDLFKREEFIGVIAQYMEENQAIIITTHEIVEIESLVDEVVILDEGQIITSFNAEE
                     LREREGKSILDKMREVYKNE"
     gene            49089..>49708
                     /locus_tag="PC471_000786"
     CDS             49089..>49708
                     /locus_tag="PC471_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2787926.1"
                     /translation="MNKLLYLINVDLRRSNKFYIAYISFFSLITLSLNLFEINKFKNS
                     KFIMNLAIEDFGGIFYGLGILSNLGFIQSILFFGILGLFIYYIFMWKREFFSNNQSIY
                     TLMMLPQNKFKICISKSIALITMIYGFLISQTATLFICKYIFNFIFRNMPIINMNFAK
                     DLYYLNLKSIPIDFISFMAIYVFLLLIAISIVNCCILFIFSYINKF"
BASE COUNT        19424 a         5333 c         8456 g        16495 t
ORIGIN      
        1 attggtggtg cagcatgtga gaagattggt tctatgacta aagaaaaatt tactatgata
       61 aagggtaatg atagatttga tacactttat aaggcattag attttattaa tagataaatt
      121 aaaaagtagc aactaaaagt agttgttatc ttctaatgcc tcattcataa taaaaaaata
      181 atttattata taataatatt aaagtttgtt tctaaggtta atattatgtg atacaattca
      241 agtgggtaaa tattatttga atgaggtgtg gggatgaaaa atttaagcaa aaaagcagtt
      301 attttatcac tttcattggt agttgtatca cctttggtac ataagataaa tgcacaagat
      361 attgagaaaa atagggaagt tagatatgaa acaaggaaag ctaataactt agaaaatatg
      421 acaatggaag aagcttttcc agatgaaaat tttagaaaag taatttgtga taaactaagt
      481 attttagatg atggaaatcc aattggaatt tcacaaaaag cgattataga atctacaaaa
      541 ggtttaacct tagggagcaa atcaataaaa aatttatctg gaattaatta ttttattgga
      601 ttagaaaatt ttagttgtag aggaaataat ttaacgagtc tagatttaag tagcaacata
      661 aatttaaaag aattgtattg taacgaaaat caattaacaa gtctagattt aagtaataat
      721 agagagttaa caacgttgca ttgtggagaa aataatctaa cagacttagt tatagaaaat
      781 tctaaattag acgaattgaa ctgtagaaaa aataatttaa agagtttaga tataactaaa
      841 gcaactaatt tgacaactct tttatgtttt gaaaatgaat tgactagttt gaacttatct
      901 aagaatcaga aattaaaaat tttgaaatgt gattataata atttaaatga tttagatata
      961 tctacaaatt tagaattaca ggatttaaac tgttctttta atcaattaga aaatataaat
     1021 ttagataata ataaagaact tgttaagtta atttgtcaca acaataaatt gcaaaattta
     1081 aatttaaaaa atcatgaaaa gcttgtaaaa ctatattgta atcaagaaca attaagtact
     1141 ttaaatgtgg aaaattgtat taatttggaa gatttaactt gtagtagaag taatctgaag
     1201 aatttggata taagtagcaa taagaattta aaatatctag agtgtgtagc aaacaagtta
     1261 acaaatttga atatagagaa taatatagaa cttttagaat tatactgtta tgacaatgaa
     1321 ataaaagctt tagatataag taaaaatatt gacttagaaa atttaacttg ttataaaaat
     1381 aaattagata gtttaaatac aagtaaaaat aaaaatctta aatacttata ttgttcacaa
     1441 aatgaattaa ctagcttgga tgtaacaaaa aatacagaac ttgtaaggct atattgtggg
     1501 gaaaatagat taaccagctt agatgtaagc aaaaacacaa aattaaaaga attagattgg
     1561 tcaaatcaaa aaaaatcaac atcaacagga ggttcgtcag ggagtggagg aggttcatca
     1621 tctacaaatg aagagtcctc tgaacctact tcaacaccat ctaaggaaaa attaacaggt
     1681 gtagacagaa atgaaacatc agtgaaaata agtcaaaaag gatggaataa agctgataat
     1741 atagtgttaa taaatgactc tagcatatcg gatgctttat cagcaacacc atttgctaag
     1801 tctaaagatg caccaatatt acttactaaa aataataact taaataaatt aacagaaaaa
     1861 gaaataaata gattagaagc aaaaaatgtg tacatagtgg gcggattaaa gtctgttgat
     1921 gaaaaagtag tgtctgattt aaagaaaaaa ggattaaatg taattagaat atctggaaat
     1981 gatagatatg aaacatcaat aaaacttgca aaagaattag acaaaaattc taacctgtca
     2041 aaagtagtag tggtaaatgg agaaaaggga ctagctgatg cagtaagtat gggagctata
     2101 tcagcaaaag aggaaatgcc aattcttctt actaatcaaa atgatgatat gaaagatata
     2161 aaagacttaa tagctaataa aaacatatca aaatcatatg taataggtgg agaatcttta
     2221 tttaataata aagaagtgaa taatacatta ccatctgtaa ctaaaatagc aggttctgat
     2281 aataataaag aagtgaataa tacattacca tctgtaacta aaatagcagg ttctgataga
     2341 acagaaacta actcaaaagt aataagtcat ttctatagca aagatacact taatgactta
     2401 tatgtagcta agaatggaat gaataaacaa gacgatttag tagatgcctt atcagtagga
     2461 gtgcttgcag gtaaaactga atctccagta gtattagttg gaaatggatt ggataatagc
     2521 caaaaagagt taatcaagaa taaaaagttt aaaaatataa ctcaaattgg tggaaatgga
     2581 aatgaaaaag catttaatga agtagaaaac ttagttaaat agattgaaag gtagtaactg
     2641 gaaatagtta ctacctcctt tttttattca ttttcagttt tttcaataat tatttttcca
     2701 tcctcaaaag taacaagaac atccctttca tcaggagaga tattcatctc tttaatccat
     2761 gacattggaa gtatcaatct gggtgtaaaa gagcctttgc cacttttatt aaatccaatt
     2821 ctaagttttc tttgctccaa taatatcatt cctttctact attaataaat gccagcaata
     2881 ataaagtaaa taccaaagca ataagagctt taaaagtaaa acttattatt atattaataa
     2941 tatttaaaac tatagtgata ccaagaataa tcatacaaat tctataaaat ttattattca
     3001 tattaaatta gaaaatgtgt tataatatta gaaagaagct acctagaagg aaggtatttt
     3061 cctcctagga ttgaaactta taattcttta atcaatctta gtattgcgag tactagatta
     3121 attaaaacta atgtgaaaga gaaagcattt ttaattttat taaatttgtt ttctctttta
     3181 ttttttcttc tttctaatct ccccactttc ttacctcctt tctatattta tattatatca
     3241 tcacgtgacg tgaaagccaa taaatattcc atatttttca ataaagttat aaaatatatt
     3301 aatataaaat acaaaatata ataaaaataa gtggatgttt acattataca cccacttatt
     3361 ttagagttat tcctcatcag atttttttat tataatctca tcttccttaa aaataactac
     3421 agccttacgt ttttcccttg taaatcccat tttttcaagt tggcttaaag gtaaagaaat
     3481 tttagccgaa taagtacctg aaccaccttt actgtaaact attttagatt ctttttgctc
     3541 caaactattt tcctcctttt ttatattgta atgtaacaaa tattaacaat aaaatgctta
     3601 ctattaatcc tattatattg ttgatattaa aatgtaaaaa taaaagtatt atgtctatta
     3661 ctatagttat agcaactata ataagacaga atttataaac tttatcactc atattttata
     3721 gaaaatgtgt tataatattt agaaagaagt cactaggaag aagattaaat tcttcctagc
     3781 ttacaaaact ataaatcttt taataattta attagtttat caattaaatt tattaaaatg
     3841 ggtaagatag agagagcagt tttaactttg gcgagtttgc tttctctatc tttttttctt
     3901 ctttctattc tactcatttt ccttttcacc ccctttctat atttatatta taacatgccg
     3961 aaccggcgaa aacaatattt atttccatat ttttcaataa agttatattg ataaactata
     4021 ttttttacaa ataaaaataa atggtgacaa aaccaaactt gccacccttt tgccaccgaa
     4081 aataaataat agaataaatt taaattaaac ataataaaac ataaacaaca aaactgacct
     4141 taataaaaca ttgaaatacc aacacttgta aaaaataaca acaataaaca aataacctag
     4201 aataatatta caacttaagt aaataataat tttgaataat aatttacgga ggtattcata
     4261 tgtttaaaca cgataaagca ttattaaaag aagtaaaagt agaaagacca aatcctcaat
     4321 atgctgtttt aatgcaagaa cagttgggtg gtgcaaatgg agagttaaaa gcagctatgc
     4381 aatacttgtc ccaaagtttt agaataaaag accctcaaat aaaagattta tttcttgata
     4441 tagcagcaga agagcttagc catatggaaa tggtagcaca aactataaat ttattaaatg
     4501 gacatgatgt tgattataat gcagttaata caggcgaaat agaaacacat gtattaactg
     4561 gtctatcacc agtcttaata aactcatcag gagctccttg gactgctaat tacgttacag
     4621 ttacagggga tttagtagct gacttacttt caaatatagc ttctgaacaa agagctaagg
     4681 tagtttatga gtacttgtat agacaaatag atgataaata tgtaaaagaa acaatagatt
     4741 tcttacttaa tagagaagaa gcacataatg ctctatttag agatgcttta aataaagtta
     4801 aagatacagg ttcaaataga gattttggag ttacagaaga ttctaaatta tatttcgatt
     4861 tgtctactcc agggccaaat cacgatacta agatagatat taatcctcct tcttttgaaa
     4921 aacctttaaa aaaataattt gatatttttt attttgtttt agtagttata aaaattattt
     4981 actagaagaa ttaattatat gaaaaattaa atatgtatag ttaaaaatgg gtatacactt
     5041 attataagtg tatatctatt tgtttattgc aaaacatata ataaaataac aaatgaggtg
     5101 gtattttgga cagtaataga aaacctaata atctaataaa tgaaaaatct ccatatcttt
     5161 tacagcatgc ttataatcct ataaactggt atagttggaa tgatgaggct tttaaaaaag
     5221 caaaagaaga ggataaacca atatttttaa gtgtaggata ttcaacatgt cattggtgcc
     5281 atgttatgga aaaagaatct tttgaagatg aagaagttgc tgaaataatg aatagaaact
     5341 ttgtggcaat aaaagtagat aaagaggaaa gacctgatgt agatagtgtg tatatgacag
     5401 tttgccaagc tatgacaggt agtggtggat ggccaatgac cataataatg acaccagata
     5461 aaaaaccttt ttttgcggga acatatttcc caaaatattc aaggtataat agacctggag
     5521 ttatagattt attagagaat gtatctgaaa agtggaatac aagcagagat atattaataa
     5581 aatctggaga tgaaataata gaagctctta aggatgattt tggtgtaaaa aatactgagg
     5641 gtgatttatc aaaagagatg ttgagttcat cagttagagt atttaaggct atttatgatg
     5701 aaaagtatgg tggttttggt aatgctccta aatttccaag tcctcagaat cttatgtttt
     5761 taatgaaata ttatagtata gaaaaagaca aagatgtgtt aaaaatggtg gaaaaaactt
     5821 tagatggaat gtatagagga ggcttgtttg atcatattgg atttgggttt tcaagatact
     5881 ctactgataa aaagtggttg gctcctcatt ttgaaaaaat gctatatgat aatgctatgc
     5941 ttactatagc ttttttagat gcgtataaaa taacaaagaa agagttgtat aaagaaattg
     6001 ctataaaaac tatagattat gtagttagag agatgaagga taaagaaggt gggttttatt
     6061 cagcacagga tgctgatagt gaaggtgaag aaggtaaatt ttatacattt aatcctcttg
     6121 agataattga agttttgggt gaagaagatg gaattttctt taataattat tttgatataa
     6181 ctagtagtgg aaattttgaa ggcaaaagta ttcctaatct tataaaaaat aaagaatatg
     6241 agagacataa tgaaaagata gctgacctta gtaaaaaggt atttgaatat agaaaagaaa
     6301 gaacatcttt gcataaagat gacaagatac ttacatcatg gaatgcattg atgatagttg
     6361 ctttaacaaa ggcatatagt actttaaaaa atgacatata tttagagtat tcaaataagt
     6421 gtcttaactt tataaataat aatcttgtaa atgagtcagg tagattatta gctagatata
     6481 gagatggaag ttccgactat ttagcatatt tagatgatta tgctttttta atctgggcgt
     6541 atatagagct ttatgagtca acatttaata tgaaatattt agaaaaggct ttaaatttga
     6601 atgaaagctg tatcaacctt ttttgggatt atgaaaaaag tggattttat atatatggta
     6661 aagatagtga aaatttaata gcaaggccaa aagatttgta tgatggagct attccatcag
     6721 gaaattctgt tcaattatac aatcttataa gacttgcgaa aattactgga gataatagac
     6781 ttgaagaaat gtcatataaa caattaaaac tgtatgtgga taatgtaaag agttctccag
     6841 ctggctacag cttttatatg ctttcattga tgtttgaact ttattctact aaggaaatta
     6901 tatgtatttt taaggaagat tctgatttaa ttgcctttaa agaattgata agtgaaaatt
     6961 ttataccaaa tgcaactttt ttagcaaaga aatacaatga agaaaacact attataggtt
     7021 ttttaaataa ctatagattg aaggatgata agattagtta ttacgtgtgt caaagtaata
     7081 gttgtagtca accaattaat gatttacaaa aattaaaaga tatgatttta ggagtagaat
     7141 aatcataata gtgaaaaagg agatgcttta aaataaaagt ctaatcatct cctttttttg
     7201 tacaaaaaac agaaaaagag ttcctgagaa cacctaatct atgtaatgat tgttttgtta
     7261 aaaaataaaa atgacattat agttatataa aatattgtac tataaaaaag tagtattata
     7321 gatgcgatag tattaatcgt ttttatttac tctctttctt ctttttgcaa attcttcaag
     7381 tgcttcagat ttaattttat ctatttcttc atattccata aaattagcat cttcttcaaa
     7441 gccatcttgt tcttcaccat taaaacgata catttttttt agtaaactcc ataaggtctc
     7501 taattcatct ttggtaaatt cgtgaaatac atctgccata aagtttattc cagtcctact
     7561 acatgttacc ataacctttt ttcctaagtc tgtaacttta acattaattg ctctcttatc
     7621 atgaggactt ggaattacat caacatatcc atttttttca agattagcaa ctagtctatt
     7681 tatattttgt ttacttgtac ccatttttct tgcaatatta tttaatgttg tttcttcctc
     7741 tggtaaatga agaattgata aaatagtcat atactgtctt gaagtaagta taccaaaata
     7801 tttatctcct tctatttgta ttttgtttgt aagggtaaaa agagttgcat atgtttgatt
     7861 cattaaaaag agttctttta gttcatttga ataatccatg aaatatatct cctttatttt
     7921 tagatgttgt tttgtcgaaa acagaatatc atgaattaat tctaatgtca atatggtgtc
     7981 aaaatatatt aaagtattac ttttttatgt aactattaat taattaaatt attgtataat
     8041 tttatgtaaa aacttctatg taccaatgat aacttctatg aggaattaat aattcttaaa
     8101 ttttttagaa agattttgac ttgaaaatac ttgtaatata atatataaat taatatactg
     8161 aataaaatca atctggaggg ataattatga agtgtacaaa ctttactttc aaaggagagg
     8221 aaggtttaga tatatataca tataaatggg aagatgaaaa tataaaaaaa ccaaaggcag
     8281 ttatccaaat tgcacatgga atggctgaaa ctgcacaaag gtatgagact tttgccaagg
     8341 tacttactaa aaatggttat atagtatata tcaatgacca tagaggtcat gggaaaactg
     8401 caaagataat agaaaatgtt ggacatttag ctgaaaaaga aggatttaga tgtcttgtag
     8461 aggacatgta tactttaaca aatattataa agaaagaaaa tgaagatctg ccaatttatc
     8521 tatttggaca cagcatgggt tcatttgcaa gtcaaagata tataatggat tacagtaata
     8581 atttatctgg tcttatacta tgtggttcaa atggaaagca aggaatcatt ctaaatcttg
     8641 ctcatctgat aatcaatcgt gagattaaaa agtatggaag acgttctaaa agtaataaaa
     8701 taaataattt gatatttggc ggcgaaataa taagaagaaa tgaaaagact aagtttgact
     8761 ggttaagtag agataaagaa caagttgaaa aatatataaa tgacccattt tgtggagtag
     8821 tgtgtagttg tggctttttt tatgatttag ttcaagggtt aaaagaaatt gaggataaag
     8881 aaaacttaaa aaaagtgcca cttgatatac ctatttacat aatatctgga gataaagacc
     8941 ccataggaaa aaatggtaaa ggagtcttaa gactaaggga tagatatata aaattaggag
     9001 taaaagatgt aacttgcaaa ctttacaaag atggaaggca tgaattgttg aatgaaataa
     9061 atagagaaga agtttttgag gatataattt gttggttaaa taataaaata gaaatattgt
     9121 aataaaaaaa tataatgata tatttacttt aaatttattt atatataata tttgaaaagt
     9181 ggattatgta tatgcaaagt agaaaagaga gagcccagaa gtgatttttt gctataatgc
     9241 catttattga ataggtgggt gttattataa ataaattatt aacactcagt attattatca
     9301 ttgtatatgg aatttatttt atttaaaatt ccttgaaagt ttaaatatag taagttgtag
     9361 taaagagaaa atataaatat ttaaatgcta tttaatgtga atctattaat taggttatac
     9421 ttaaacttaa ttaatagatt ttatatttca agacaatgag atagggtttt agattaaaat
     9481 ataaccaatg gttaaattac agacattttg ttaactttcc ctcgatttac tagtaaatat
     9541 tttttttata tacttaaatt acaacagatg tactagggag gtatatatgc aaattaaaat
     9601 aggtaaagtc atacagtgtc ttagaaaaga acgaaattta actcaagaac aattagcaaa
     9661 gtttataggt gtgtcaacac cagcagtatc taagtgggag agtggaaatt cttacccaga
     9721 tatagaatta ttgcctttat tagcagactt ttttaatgtg tctattgata agcttttaaa
     9781 ttacaagatt gatttaagtg aagaagaagt aatgaaaatt tataaagaat tagaatcagg
     9841 ctttgctaga attgaaattg atttatcaac agaagaacca aaagaagaat ttagacaaga
     9901 tttagaatct gtaaaaaagc tttccaatat gtatatagag aagtatccta aaagctattt
     9961 attgaagcta aggatatgtt cgctgtatca gatgtattca tataaatttg gtaagagtga
    10021 acttaatgac agagtaaaag aaacaacgaa tattcttgaa gatatagtga gaaatacaga
    10081 tgatatccaa ataaaagaaa cagctcttat tatattatca aacgcatatt gtatgttgga
    10141 ggattatgaa aaggcagagt tgtatttaaa tatgatacat aagtcaatag gggatacaag
    10201 tgttaattta gctatgatat atttaaagca aaatagactt gaagaagcag aaatattgct
    10261 tcaaaataaa ttgtttagta atgtatttaa tataagcatg gattgtaagg gaataattaa
    10321 tgtatacaaa aatcaataca aggaattaaa gaaaaaattg gagaatagaa attttaataa
    10381 aaatgcaatt gagagagaaa tggagtatat aaaaaataag ttgctggggt atgcaaatct
    10441 ttctttagaa ataaaaaaaa tgctcagtga agataaaggt gcatttttta gtatgtatat
    10501 ggattatatg gaattatctc taattttttt attttttaac atgaaagaag aagcaaaaaa
    10561 agctttatat agtctaaaag aaatattaga aaaatatcct atacatgaaa gtttggatgt
    10621 aagtcaaatg agattttttg ataaggtaga gtctaaaaat ttatatactt ttaatatata
    10681 tacaaacttg ttaattgtac ttaatgatga cagttataat gagcttagag aagaaccaat
    10741 ttttaaggat gtaattgaaa agattttaga tatggaaaaa atgttgaaaa ataaagaata
    10801 agtaaaaatt taatatagga cttaaagatt aatcaaatta aaagaaagtg tttcaaaata
    10861 aaaatttgaa tcaccttttt tgtcggtctt aaacagttat ttagaaaaat agaggtttca
    10921 aaatagaaat ctgaattatc tgtattatta cacaaaaata gaaacctaaa tttataaaaa
    10981 tagaataaaa tttgttatat aaaatgtaat tttgtctata aaatctcacc tgttttctta
    11041 actttaagcg tgaaaaagag gatgtatcat gaatttttta gttcatgatg catcctgctt
    11101 tgtatgaaat ccaatatatc tttattattt aaacaatgga aaagagtctt atttatattt
    11161 tgagacatct cttttattta attatgaagt tttatacagt tcaataaata ggaattatat
    11221 ttagaaactc atgacattat ttcttagata gtgattaaat ttatcatatt ccattttaga
    11281 taacttttct gggtaagaca ttacaatcag gttatgccca ttaaattttt tatggtatgg
    11341 tggatgaatg tgttcataat tatttgtctt atattttact ctttcataga acgccttgcg
    11401 ataaattgaa atctcatcaa ttggaaggtc aatttctaaa ataatagttt ttcctttctt
    11461 atttaaaagc tctaacgctc tttgcccata tctattattg cgaatttctg gaaaaatgca
    11521 gaaatgttct acatagataa agttatctgt ttcccaacat aaaattatgc cgacaaactg
    11581 gtttttatca taaattaaat tgaattgata ttctttattt ttcaaaattt ctgcttgcaa
    11641 agaagatttt ctttgtttat gaaatgggaa actaatttta tataattcca tagccttgta
    11701 atataattta ttttcactgg ttcttaaccg ttcaaatttc actttactcc tcctaatcac
    11761 gaaaattcca atttatttat gagttcattt taccatagtc agtatttgca tacaatgtgt
    11821 tttaagttga gtgtcatttc tttcttttat ttatgcttat atctcatagt ctaagagata
    11881 attgcttaag acataactaa aaactattat attaagcttt gataatctaa aaacagtata
    11941 tttatggact tttctaggtc taaaagtaat cttaataatt ttagaagtta caaaaaagtt
    12001 acataaattt ggaaagctat aatatacata tataatataa ggttaaaact gtaatgaata
    12061 atattaattt aaaatttgaa catggaagga gattattatg gagaaaagag aaaagaggat
    12121 aataatatca tctttattaa gtgtatcaat tttaatgggt ttggcaagta tatattctat
    12181 attaaataaa gaagacataa gtttaaccgt taaaggtcaa gagcaaaaag tatcttcctt
    12241 taagaaaaca gttgaggaac ttttggatga acagggtgta aagtataatt ctgaagataa
    12301 gattaatcca agtttagata cagagttaaa agatgatatg aaaataaaag ttgttaaggt
    12361 aactaaaagt aaaaaagaag agattgaaaa aattccattt gatacaaagc atgtaaatga
    12421 tagtaatttg ttaaaaggaa aatctaaagt ttatcaagaa ggtcaagaag gagaaaaaaa
    12481 actagtctat aatttaactt accatgatgg caagttagtc aaaaaagtct tatcaaaaga
    12541 agtaatatct aaggagccaa ctacaaaaat tataaaatat ggaactaaag aaaaagtact
    12601 aatagcatca agaggagcaa atataagagg aggcaaacat atgaaagtgg ttgccactgc
    12661 atatgcagga gatacaataa catctactgg tacaactcca agatggggtg ttattgcagt
    12721 tgacccacgt gtaataccat atggaacaaa agtatatata cctaaactgg gtatgacttt
    12781 tgtagcagaa gattgtggag gtgcaattaa aggtaataga atagacattt ttatgaacag
    12841 tgaaggaaaa gcttcaaatt ggggaaggaa aagtatagat atatatctac attaaattag
    12901 tgaatagaat aactttactt gaaggacaaa aaagtataaa atatgaatta actaaactgt
    12961 tagattattc ttaaaacata aaaatatatc taagggggga taagatgaaa atagtctgct
    13021 taggagacag tttaacgtat ggatttggag tatcacgaag taattcatgg acaaacattg
    13081 taaataaaga gactcgatta gaaatagtaa ataagggtat aaatggtgat acaacaagtg
    13141 gtatgttagc taggtttaat gaagatgttg taaaaaactc tcctgatata gtttttataa
    13201 tgggtggcac caatgatttt attgctggtg cgggaaatga aattattaat tctaatataa
    13261 tggctatggt gcatcagtca tattcaaaaa atattatacc tataataggt ataccattaa
    13321 aaccagatat tccaaatgtt agggaagatt ggagttgttt tactgatttt aatatagtat
    13381 ctcaaaactt agaatcatat agttattgga taaaaaaatt ttgtatgaca tttaatacta
    13441 atttcgtaga tttttattca gaatataata aaaatatgga actagaagga tataaaaaat
    13501 tatattttga tgggttacat ccaaccaaag agggtcatag aataatggca gacattttta
    13561 ttaattcaat taataagtat atagaagagt tatagattac attaaaatgc ttgtttatgc
    13621 gtcagttgca ataaatacat acagtagaga ggggtttatt atggattata ctaaaactta
    13681 tgaagagtgg ataaagggtt catattttga tgaagataca aagctagagt tggagaatat
    13741 aaaaaataat gaaaaagaaa tagaggatag attctataag gatttagaat ttgggactgc
    13801 gggtcttaga ggaataattg aagcaggaac gaatagaata aataagtaca cagttagaag
    13861 agctactttt ggattggcaa actatatatt agaaaacaca acaaaagaag aaacaagtag
    13921 aggagtagta atagctcatg ataatagaca caagtctaga caattctgta tagaatctgc
    13981 aaatactttg gcagcatgtg gtataaaagc atatatattt gatagtttga gaactactcc
    14041 agaattatct tttgctgtta gaagcttaaa tgcaatcgct ggtatagtca taacagcaag
    14101 ccataatcct ccagaatata atggatataa ggtatactgg gaagatggag cacaagttat
    14161 gccagagatt gcaaatgcaa taacagagaa agtaaatagt atacatgatt atagtacaat
    14221 accaacctta acggaagcta ataaaaattt ggtagttttg ttggacgaat cacaagatac
    14281 taaatttata gaagcagtaa aaagccaaat tataagaaaa gatttagtta aaaatgtagg
    14341 taaaagcttt aagatagttt atacacctct ttgtggaaca gggaatgttc caataagaag
    14401 agcattgaaa gaagttggtt ttgaaaatat aattgtagtt ccagaagaag aaaatccaga
    14461 cccaaatttt gcagggcttg attatccaaa tccagaagag aaaaaagctt taaatagagg
    14521 tatattactc gcaaaagaaa aaggagcaga ccttgtaata gcaactgacc cagattgtga
    14581 tagagtaggt gtagcagtaa aaactactac aggtgagtat gctttactta caggaaatca
    14641 aattggtggt atgctgacac attatataat agaaggatta aaagaaaata ataaattaaa
    14701 agaaaatcct acaataataa aaaccatagt tacatcagaa tttggagcag atatagctaa
    14761 agcaaataat gttgatgtat taaatgttct tacaggtttt aaatttattg gtgaaaaaat
    14821 aaaattattt gaacaaaata aaaatagaag ttatgtattt ggatatgaag aaagttatgg
    14881 ttacttagtt gggactcatg ctagagataa agatggtgta gtttcttcat tgttaatatc
    14941 tgaaatggca gcattttact attcaaaagg tatgagttta tatgatggct taatagaact
    15001 ttataaaaag tatggatttt tcaaagaaca gactatatct ttgactttaa aaggtataga
    15061 aggtgtagaa aagataaaag aaataatatc ttattttaga gaaaatcaaa ttgattatat
    15121 aaatagtatt aaagtagttg ataaaaaaga ttataaaaat ggtatagata atcttcctaa
    15181 atctgatgtt ttaaaatact ttttggaaga tgaatcttgg gtagctataa gaccatctgg
    15241 aacagaacca aaacttaaat tttacatagc tgtaaaagga gctagtgata ttgaagcaga
    15301 taaaaagatt caaggtttga aaaagtatat agatgacatg gtcgaaaaat taaaataaaa
    15361 tattattatt ttataagata attttgtaat aatatttatt tagctgaaaa ttataattta
    15421 ctttcattga gtagaatatg gaaatgttag tagaattgtt attttcagct tttatttgtg
    15481 ttatttttac ttaaataatt atattataga atgaatctga gtataattaa tattgtataa
    15541 tgaaattaat ttgatagaaa aggggatatt tatgtttggt atagataaga aattgattga
    15601 atatataatt gcaaaatcca attacagtga attaagttca aagtcagcag aagtatcttt
    15661 cttcttaatg ttgtccatat ttcctttttt aatatttaca ataagttcta tagcttatat
    15721 accgattctt catttaaata agtatatagc tttgtttaga aatatgatgc cagaaggtgc
    15781 ttttgctgtg ttatcttcaa tcatagtttc tgcaatagac aatagaaacc tgaaattttt
    15841 agcagtcagt tttgtactta ctatgtggac attttcaaga gcagtaaaag ctcttattaa
    15901 aggaatgaat agagcctata aagttaaaga aactaggtct ttttttaaaa ttttatctat
    15961 atcattttta tttacaataa tgcttttggt ccttattttt ttatctatga tatttttagt
    16021 ttatggggaa aaaataggtt attttatttt taatcttgta ggattggatg agatatttat
    16081 aaaaatatgg gatattctaa gatacactgt aggtataata acaataatag tgatatttac
    16141 acttttatat aaatacactc caaataaaaa gttaactatt aaagaatctg cgccaggtgc
    16201 tatttttgct acttttgctt ggtttttagt atcattttta tattcttatt atacaaatta
    16261 ctatgctaac tatgaagtaa tctatggaag tatagctgaa ataattgtac ttatgacctg
    16321 gatgtatttt agtagttggt ctatcgttat tggctatgag gtaaattcaa gattgtattt
    16381 tagaaagata agacatgaaa tgttaaagta aagtatttaa aataaaagag agtatttagt
    16441 attataaatg taattgaatt aataaagaaa tattgacatt taagttaaaa agtctagaat
    16501 ttttctgggc tttttttatt tttagtataa cttgacaaat tttatttaat gggatagttt
    16561 taatttataa tcatctttaa aaagatgact attgataagt aagagataaa attggtaagt
    16621 atcaaaaaat atactaaata aaaattatag tcctaatgag ttaatttctg tattttcaaa
    16681 taatgcagta tttagtcatg tgtttttata tgcgcctgta tttctaatta ccaatatatt
    16741 agaataagct actgtaatta gatttagaaa aaagcgacat aaataattat aaacaaataa
    16801 taaattacat aaaattaaat ttattcacaa gtatatctta taaaatatgt tataattaat
    16861 agaaaattgt catataatta attaagtagt attattataa ataacattga aaggtagtgg
    16921 ttagaatatg gatacccata ataaatatgt aaattttata aaaaatatac ctgtaccttt
    16981 tttatattgt agaattgtaa agagacaaga agatatagaa tatcgagtag aatatataag
    17041 taagggtatg ggcaaagtat tacaattaga agaaggtatt tgtgataaaa atatactaga
    17101 tgtattgcct gtatttaagt ctaaaaaata tttcaaagag ttgttctcaa atgaagtgga
    17161 ttgtataaaa aggtacattc cgacacttaa aaattggata aatataaaaa aacagataat
    17221 aggggattca tacataattt tgtattttgg taaaattgta tttgattata gacagataat
    17281 cgattccttt gataaaaagg aaaaagtagc ttatattaaa gacgaagaag gcacatatat
    17341 agattgcagt gaaaatttaa taccaatatt aaataacaac attaaaacaa cgaaagatat
    17401 ctttggtaaa aatgatatag aagtatgggg agagaataca ggaaaattat ttagagatga
    17461 ttatagagaa ggagtatcta gtaaaaaaag atttttacaa aatctttttg aatatgaaga
    17521 aacatttttt atggttgaaa aatatttttt gtatgatgaa gacgaacttt taggtactat
    17581 agggatagta gacaatataa tatatagtgg ttacagtaat agaaattata actcaaaaga
    17641 tttaatgaag atgatagaac actcaattcc agagaatatg ttttataaag atgtgtatgg
    17701 aaattatatt ggatttaatt caggtttttt gaatttagct tgtatgaata aagaagaatt
    17761 attagggaag aatagttata aaatatcaga agaagaagct ttaatagata agatatttga
    17821 aagtgataag ggtgtagttg aaaataaaaa agtagttaca tttgagttaa atataagtat
    17881 gaatgatgaa aacaagtgta ttgagattac aaaaaggccg ttttttgata gctatggaag
    17941 tgttattgga ataattggaa cagcaaggga tataagtaga agaaaaagat tagaagagga
    18001 aatggataaa actaggatgg aattttttgc aaatttatcc catgaactta gaacgcctat
    18061 aaacttaata tcatcttcat tacaagtgat agaaaaaaaa gaagcagatt taatagaatc
    18121 aaatgatact ttaaaaagaa atttaggtat aataaaacaa aatggaaata gaatattaag
    18181 attagtaaat aatgttatag attttacaaa aatgcaatca ggatatttag attttaaacc
    18241 agaagaatct gatataattg catttataga ggagatatgt atgtctgtag ctgattttgc
    18301 aagccaaaat aatatacaac ttacatttga tacagaaata gaagaatttt caatgttatt
    18361 tgattcagaa aaattggaaa gaataatatt aaatttactt tctaatggaa ttaagtacaa
    18421 taagaaagat ggaaaaataa atatattttt atatgtgaaa gataatgtat ttaatatgaa
    18481 aatatctgat agtggaatag gaataccaaa agaaaaaata gataaaatat ttaataggtt
    18541 tgaacagatt gacaatgaac tttcttacag agttaaagga agtggtattg gactttcatt
    18601 agtgaaatct ttggttgagt tgcatgaggg aagtatatct ttaaaaagtc aacttggtat
    18661 tggaagtgaa tttatagttt cattgcctgt tagaagtaaa aataatattg aaaaatataa
    18721 tcataaaaga gaaatcagca atgaattaag taaaaaatta gaaatagagt tctcggattt
    18781 ataagtttaa atataaaaaa taaaatttta aaaaagtatt gacaattgat ttagaagggt
    18841 ttaaaataaa ttacaacata ataaataaaa aggcgatgaa taggaagagt aaatctaatt
    18901 cgattaccag agagaagagt ccgttggctg gaagactttt taaattagag ggattgaaaa
    18961 ctacctagga gctactttct gaagtcatct gcattagtag attttagtaa gaatagtcgt
    19021 tagctgcgtt aaagtgtaaa gtgagttttc atgacatatg aaaactaaat taggtggaac
    19081 cgcgggaata atactctcgt ccttatgtta taaggatgag agttttttgt tatttaaaaa
    19141 tataaacagt aatcataata attatagaaa atataaaagc aatgaatagg aagagtaaat
    19201 ttaattcgac tatcagagag gaaagttcac aggctgaaag actttctaag tttgagaaat
    19261 tgaaaactac ctaggagctg tggtctgaag ttactttaaa aagttttagt aagactaacc
    19321 gttaactgcg ttaaagtgta aagtggattt tatcaataaa tgataaaatc aaattgggtg
    19381 gaaccgcgag tatagactcg tccctttata tagggatgag tctattttta ttgcaataaa
    19441 agattgaaaa taaaaaaaaa tcggaaaatt ataaatttag gaggaaaata aaatggagaa
    19501 taaagtgaaa attggagtac taggatatgg agttgtagga agtggactta tagatataat
    19561 agataataat aaagaaaaaa gaaatataga aatagttgga attttagtta ataatttgga
    19621 aaaacataag gataaaaaat attctaatat aataactaat aatatagatg atatatttaa
    19681 taaagatata gatatacttg ttgaagtcat gggtggttta gaaccatcat tatcttatat
    19741 aaaaaaagca ttaaacaata aaattcatgt tgttacagca aataaagatt tgttagctga
    19801 atgtggagat gagcttgcaa aattagcaag tgaaaataaa gtaagtatta aatttgaagc
    19861 ttcagttgca ggaggtatac cagtattaaa accaataata gaatcactag aaggaaataa
    19921 tatagatagt ataaatgcaa ttttaaatgg aactactaat tttatattat ctaaaatgta
    19981 tgatgagaat ctatcttatg atatggcatt aagacaagca caagaattag gttttgctga
    20041 agcaaatcca gagtcagatg tattagggta tgatgctgct agaaaactat caatattatc
    20101 aactctagct tatgacaata gagtatattg gaaagatgtg tatttagaag gaattacaga
    20161 tatagatgaa aaagatattg aatatgcaaa aaaattaaat tgtaaaataa aattgattgg
    20221 tcaaagtaag tatgaaaatg ataaagtaag tgcttttgtt agacctgttt tagtagaaaa
    20281 agataatatc cttgctagaa tagacaatga gttcaatgct gtcatagtga atggagattc
    20341 agtaggagaa gtttcttttg taggtaaagg agctggaagt ttagcaacag gaagtgcggt
    20401 ttattcagat gttatagata taatagataa tagagtttca agtatagatt cttttactaa
    20461 agataaaata caagttaata aaatagttag agaaaagtgt ggagcacttc ttagatttaa
    20521 aaaatgtaat aaagatgaaa ttctaaatat agttgagaat tgtttagtaa aattcgatat
    20581 tttaaatgat gatgatgaac ttgctattat ggtttatgct gattctgaat atgaaataaa
    20641 caacagttta tgtctaataa aagataaagg atactgcgaa aaaatgaata aaatgttgaa
    20701 aataagttaa aaaagaaaaa atataagttt atatgtaaca tattttgacg attttaatac
    20761 tatatagtat gagatgaaac aaaacatctc ataaaaaaat taaaattaaa gggagagata
    20821 tgttatatgg aaaataaaaa atgttattca gaagattggt atgaaagagg agaatctaca
    20881 gctaaatggt tccaaaatga tagagaagaa tatgaaagag aagcatatga tgaagataga
    20941 gaaagaagag gttcaaactg tggatgttca gattcaggag aaaatagacc tagaaactgt
    21001 gaaagattta gaagagaagc tgagataaga gaaagagaag caagagaagc attctgtgaa
    21061 tcttcagaga aaaagaaaga ggcattagca tatgaatgtg aagctagaaa attatgggaa
    21121 gaagcagaaa aatactggga tgaatattca aaatacaact ataaaggaat cgaatattta
    21181 gcagaagctg ctagattatt tgatgaaggt atggaatgtg aagctagaag aaatggaaat
    21241 aatggaggaa acaataataa ttgttgccat aaatgccata aatgtaattg taactgctgt
    21301 agaaaataaa aaaattaatt gacaaatcaa tagttaaaat atatcatatt atgtataata
    21361 aagagaaaat tcagaaaaaa ataatgatta gaagaaatat aagtatattt attagacaaa
    21421 tagtgctcag ttagtgaggt gagtacaata tgattaaact cccatcctct agcttttgat
    21481 agagggtggg agtttttatt tatttatata tcttaaatga ggataatatt gtaaaataaa
    21541 tgagtatgag attgtaaaat taggaggtaa aaatgaaaag tattaaaaca tcaagtatga
    21601 aagaaaaggt agatgaaaag atattaagag agcaagtaag ttctataata gaagatataa
    21661 gaaataataa ggatgttgca cttaaaaaat ataatgagaa gtttgataga aatacaagag
    21721 atgaatttag aattactaag gaagaaataa aggaagctta taagcatgta gatgacgaat
    21781 ttataaataa cttaaagatt gctgccaaaa atataagaga atttgctaaa gctcaaaaac
    21841 taagttttga aaaccctttt gaaaaagaaa tttatccagg tgtaatctta ggtcaaacta
    21901 atattccaat agagtcatgt cttgcatatg ttccaggagg tcagtatcca ttgttttcaa
    21961 cagctcttat gcttataata cctgcaaaag tagcaggagt aaaaagaata gtggcatgtt
    22021 caccaactat gaaaaatact gaaaagataa atcctaagac attggtagct atggatatag
    22081 caggtgctga tgaaatatat gcaactggag gagttcaagc tatagcagca tttacatatg
    22141 gaacagaaaa gattaatcca gttgacataa ttgtaggacc aggaaataag tttgtaactg
    22201 aagctaaaag acaatgttat ggacaagtag ggattgattt tgtcgctggg ccaagtgaag
    22261 ttcttataat agcggatgaa acttcaaatc cagtttatat tgcagctgat ttattagcac
    22321 aatgtgagca tgatttaaat gcaagaggaa tattattgac aaatagcttg gaagtagcac
    22381 aaaaagttga aaaaaatata gaaagtatgc taaaagattt gccaacaaag gacatagcat
    22441 atagttcatg ggaaaataat ggagagataa tattggttga tgatatggaa gaagccatta
    22501 aaataagtaa tttttatgca ccagaacatt tagaagttgc agtaaatgag tataatgata
    22561 tttgtgatag attaactaac tatggttctt tatttattgg aaatctttct gctgaggtgt
    22621 ttggtgacta tgtttcaggt accaatcata cattaccaac acttaaagca tcaagatata
    22681 ctggtggtgt ttgggttgga acttttatca aaacttgtac aaagcagata tttaatgaaa
    22741 aagctataca atcattagca cctgtagctg aaaagctggc aaaagaagag ggattatatg
    22801 cacatgcaaa ggctgctgag gttaggttta aaaagtagta aaaaatttta ttttggcagt
    22861 cgagacttag tattagactg agataaggaa aacgctgaga acctgaataa atactatatt
    22921 ttgtgggtct cggcgcttct tttatatctg tatataatag aataatacta tctgtgtgtt
    22981 taaatactaa cttaatattt cttttctaaa taatgtttat gaaatagtta atatttttag
    23041 ttaatacttg aaaaaaactt ttgctaactg tgcaaattca tctgcatttg tcaatatagc
    23101 agggtgtcct ccctgactaa aaatgtaaat acttgctttt gaaataaaag ttgccatttg
    23161 cttgtactct tcttcaagat tatgacgaca catttcgtct tccttacttc ctgtaaataa
    23221 aattggcatt tctaatttac acaattcttt atgaaacaaa gggcgtttct cgtttgcaca
    23281 ttgcaataga gcttttgtat caagattaac aacattctcc caatcttttc catgacacca
    23341 ttcatagaat tttcgtgctt gaatatcttg ctttgatttt tcccttccag tgattagatt
    23401 atctatgaag ttatcattaa gagtacgtcc atcaaagcta tctgctataa ttgcttcaac
    23461 taaatcggga cgttctaatc cagtattaac agctgcccat gctcctccac ttgttccaat
    23521 taagccaact ttaccacaat tcaaatgttc aatcagtgct attgtctgta aggcttcatc
    23581 atgccatatg tcaggagaaa attgagaaac tctatccgac tgtccattac ctaaaaaatc
    23641 aattaagata catctaaaat tttctgcata taatggcaca agtgctttaa acatattaga
    23701 agaaccagta ttaccatgca ggaaaattat cggtttacct tgtccatatt ctttataaaa
    23761 acaagaatta ccttgatact gaaaatatga catatatttt cctccttttt atttgttaag
    23821 ttaattataa caatataggt atgaatgaaa aacctcatgt tctttcttag aataaaaatg
    23881 tgccacgcat aaaacgtgac acatttctaa ttataaattt tgctgtttaa gtattctttg
    23941 tatgcttttc tctgtcaaaa agtatttctc tgataactgc ttgatactca ttccattttg
    24001 gaaatttata cagatttgag aattacgaga agctaagact tgtttggtgt ttgtgttttc
    24061 accccaatgc tttttgtgct cttgcttttt gggaatataa atcactttac catctatata
    24121 ttcttgcact tgctcaatca gttctttagg cagtaagtca atcgaatttt gatagctcat
    24181 tttgcacctc ctaaataatt attctttagg attagcaaag gctactgcca ctcacaatta
    24241 aaaataaaat tttttattat gtacaatagc ctttgctatg cacaagttcc aattcccata
    24301 aactaaatac tcctttctgg tttgaaaaat tcatctgaac attgatttcg cttttaagat
    24361 agagttcaat gacaaattta catttttatt atatatattc agatagataa ttgcaactgt
    24421 ctttgtatga ggatatttta ttatagtcaa tactataata tgtatttata tcatcaaact
    24481 aatatgaaca gttgaaagga atatgaacaa taaaacttag tcaggaagaa taattatgaa
    24541 gcagacaata gagaaatttg attttaaagt tttattgaaa atttattgac aaatttaact
    24601 tgtaaatatt atactaaact taataaataa agtttataaa gtaaggtgtt gtctatgaaa
    24661 aatatagttg gaaaaccgca aacgataaaa aaagtaaatg aagatttaat aaaaaacatt
    24721 ataaaaaatg aaggtcctat aacaaaacca gaaatttcaa gcattactga attgagttta
    24781 gctacagtta ataagattgt agagcaatta gctttgaaaa atgaagttaa agtaagtgga
    24841 ctaagtgaat ctactggagg aagaagagct caattatttg aaataaatgc aaacttagaa
    24901 cacataatag ctttatatta ttatagaaat tgttgtatag gggtagttgc caatttatta
    24961 ggtgaaataa tttatcaaga agaatttaat ataagaatgg atacgtatga aaacgttaac
    25021 caagatactt tttttgttat agatactcta ataaaaaagg aaaaaggaaa caatataagg
    25081 gctattggaa ttggtgtccc aggagttgta aaaaaaggtg tagttaaaaa tataccaact
    25141 ataaaaagtt gggaaggtat aaatttagaa aatttaataa agagtaaata caatactaaa
    25201 gtgtttatag aaaatgatac aaatattact actattggga tttatcaaaa acagtataaa
    25261 gacaaatata aaaatatggc tcttatgtat ttagaagatg gtattggttc aggtatagtt
    25321 ataaatggtg aattgtattt aggttctaca aattttgctg gagaattaag ttatctaaat
    25381 atagaatttg gtaagtataa ctctaacaac ttaaatcttg aagattatat tatgagttta
    25441 agaaaaagat atttagaaag taaagataat aaatttaaga aagaaatttt atctataata
    25501 tcaaacttta taaggactct tgtatgtgtc ctaaatccac aagctatgat tatacagtgt
    25561 agtatattaa ccaagagtga catggagttt atagaaaaag atgtaagcaa taatgttgga
    25621 gaagaaaatt ttcctaaatt aataaaagta gactcattga gagaaggaag tataaatgga
    25681 gtaattagta tgtgtttaaa agaaactgac tatcaatatt ccttatctaa taaaaaagga
    25741 ggatattaat gtgaaatatt tggtttcaat agatggaggg ggaacaaaaa ctaaattttg
    25801 tgtaagtgac ttagatggaa acatacttaa agaacatacc actggttcaa ctaattataa
    25861 gtcagttgga attaaaaaaa catatgaaaa tattaataat ggatttaaaa aaatattaaa
    25921 agatttatat atcgattatg acgatattga gtacactgta tttggtatat ctggttgtga
    25981 ttctcctaat gattataaga ttataatgga tgaaatatta aaaattggga ttaacaaaga
    26041 aaaaatatat ctggcaaatg atgcagtttt agctttttat gcacaagctg attcccctgg
    26101 cttagtaatt gtggctggga caggttcaat tatactggga attaaagaag atggagaaat
    26161 atatagagtt ggtggatggg ggtataattt tagtgatttg ggttctggat atgatatagg
    26221 gagaaaatta ttaaagaaag tattgcttta ttgtgatgaa tgtcatgagt atagtgattt
    26281 atttagttgt gtcttggact tctttggagc aaatagtttt gaacaacttg catatatgat
    26341 tactgatata aatgataatg ttgaaatagc aaacttagct tcattagtga ttgattgtgc
    26401 cgaacaaggt gataaattgg ctattgaaat attaagagaa tcatcaactg aactatctaa
    26461 attagctcaa gtaatattga gaaaaatatc taatccaatg aaaatggata aaagcgaaat
    26521 aaatatagtt ttgtctggtg gtacattaaa taaaactgta tatgctcaaa tgttaatgaa
    26581 taatttaaaa acagaaaata cagataaaaa tttaaaattt atatctcaag aaaatcagcc
    26641 agtatatggg gggataagac ttgctatggc attagctaat aggagggtca agcatggata
    26701 gaaaaaaggt aatagtacaa atttttactg gaggttttaa ggctaaagaa ataaagttta
    26761 atgatataaa atgtaaacta ctagaattat tggactgtat tgatatagaa aaggttatca
    26821 tgggatggag tgtagatgaa gaattatatg tagaaactaa aaaactatta aataaatata
    26881 atgttgaact atatctttgg ttgccatgtt tttctgaaat aggattactt gaaaaaagta
    26941 atttactaat tgattatatg gggaaggaag tcaagggcta tgctttacaa gaggatgaaa
    27001 attttgagtt ttattgccca aataataagg aaaatataaa aaatataaag aaaatatata
    27061 gtacttattt tagtgatatt aaatttgatg gaattttttt agataaaata aggtatggtt
    27121 ctttttcgaa taaactaaat ggtgttttta attgtttttg taaagattgt ttaaatatat
    27181 ataaaaaaaa taatattgat aaagagtcgt taatgagaga aatgaataat gtgtctaagg
    27241 gagttataga taatgaagat actccatttg gtataacaga atacattaat ggaaaataca
    27301 aatttaaaaa taaaatatgg gaagattttt ttgaaacaaa aaatcaaaat atatacaagg
    27361 cattattaga gataagtagt tattttaaaa gtaagggatt gaaaataggt atagatgctt
    27421 tttctccttt tatttcttac tttacaggtc aagatttaga aaaattacaa gaagtagcag
    27481 attttataaa accaatgatg tatagaacta caaaagctcc agctggttta ccatttgaat
    27541 ttgaaaggtt tattgatgaa tccatacata agaataaaga gattggaaaa agaaatttct
    27601 tagatatttt aggaattaag gagtatgaag gagatgtata tcctatagaa tttgtactag
    27661 aggaaatcga ttttatgaca agaaatctta ataaagatat atatgtagga gttgaaataa
    27721 ataaaaagga aattatatct cctgttacac ctaagtacat aagtgaaaat atttcaaata
    27781 taaataagac aacagtaaaa ggatatgttt tatcttggga tttgttgtca gcaccaaaag
    27841 aaaatattga tgaaattata aaatggtttc ggaattagtg aggtgataaa gtgagtaaga
    27901 tattggaaat acaacaaata tataaaagtt ttggaaaggt agatgtttta aaagggataa
    27961 attttgagtt agaaaaggga gaagtaagag ctcttttggg agctaatgga gcaggaaaat
    28021 ctacacttat taaaatttta ggaggagttc attctcaaac taaaggtaaa gtaattttga
    28081 ctggtaatga aataatgttt aaaaatccac atcattctaa agaaaatggt attagtataa
    28141 tttatcaaga attaagcttg ataccaacgc ttacagtact tgaaaatatt tttcttggta
    28201 gagaaatttt aaaaggaagt gtgtttttaa acaagaaaga catgcttgag gaatatatga
    28261 aaatttgtga ggattttgat tttaatatag acccacatat agtagtttct aatttaagtg
    28321 tagcaaacca acaaatggtt gagataatga aagctgtttc atgtaattct gaaattatca
    28381 taatggatga acctacaaca tcacttacta ataatgaaaa agatgggcta tttaaaataa
    28441 taagtagact aaagtctaaa ggtaaaacaa tagtctatat ttctcacata ttagaggaag
    28501 tttttaaagt atgtgatagt gcaagtataa tgaaaaatgg aattatgatt ggaacttatg
    28561 atgtagagga tttaactaaa ttaaagataa ctcaacttat gacaggaaat gataatcaca
    28621 aacttattga gaagaaggat tatacatttg caaaatatga agagccacca gtactagaag
    28681 taaagaatat aagctggtct aacaaaataa aaaatgtttc atttaaagtt tatagaggcg
    28741 aagttgtagg tcttgcaggt cttgtaggtt caagaagaac tgaaattata aatttgatat
    28801 ttggtataga taataaagat agtggagata tttttatcaa tggtaaaaaa gtagacataa
    28861 cttctcctaa ggttgctata aaaaacaaaa taggattgat tcctgaagat agaaaaaatc
    28921 taggattaat tttagaacaa gctatataca caaactcatc atcagtacaa attgataaat
    28981 tcaagaaatt tttgtttata gatagaaata aagaaattga gttttctaaa aaaggtgtta
    29041 aggaattagg aataaaagtt agtgatgtaa agcaagaagt aaaagagctt agtggtggaa
    29101 atcaacaaaa agtagtagtt tctaagtggt tagagaaaga tttagattta ataatatatg
    29161 atgagccgac aaagggaata gatattgctg caaaagaaga tattttcaat acaatcaaag
    29221 aattttcaag taaaggaata ggagtaatat ttatatcttc tgatttagaa gaagtaataa
    29281 gagtaagtga tagaatttta gtaactagag aaggttctat tgtagatgat ttaaaaaatg
    29341 agaatataca ggttcaagat ataatgaaca gtatatttaa tgtataaaaa atgaaaatgg
    29401 aggactgaga tgaagaaagc ttttgagaag ttaaatttaa gagattatgg agtattatta
    29461 ggttttttag ctttatgtat agctataagt attgcaactc catcattttt aggtaaacaa
    29521 aatatactta atcttttacg acaatcttct ataattggaa taatttcagc aggtatgact
    29581 tttgttataa tatctggaaa ctttgatata tcagttggag cagtagcagc tcttgcaggt
    29641 gctataacaa tgaaatttgc gactggtgga actaatttgt ttgcatccat gttcttaggt
    29701 gtagcgattt gtgcagtaat aggattaatc aatggtatag tagttgcaaa gataaatgta
    29761 ccatcattaa tagccactat ggctatggtc actattgtaa gaggattgct gttgatgctt
    29821 acaggaggat atccaattac tgaaaatata ccaatacttg attacattgg aaatggatat
    29881 ttgtttaata taccaatacc agtagtaatt ttctttatag tggtgttagc ttcgtttatt
    29941 gtacttaata aaactaagtt tggtagatat gtttattctg taggagggaa tcaagaagca
    30001 agtaaactta atggtataaa tgtagattct cacaaagtga aagtgtttat tattaatgct
    30061 gtacttgcag gaatagctgg aatcgtacta acaggaagac ttgggactgc aactgcgatt
    30121 gcaggtgaag gatatgatat ggatgcaatt gcatctgtag taataggagg tacatcagtg
    30181 gcaggaggtt ctggttctgt acttaaaact gttataggag tattacttat gagtgttata
    30241 aacaacagtt ttaatcttct tggaatagat gtatatttcc agtacatatt taaaggatta
    30301 ataattttag cagcagttgg atttgattct tatagcaaga aaaaacttgc ttcaagataa
    30361 aaaataaggg ggattttttt atgaaaaaat taatgaaaca tttagcattg ttgctatcac
    30421 ttgttatgat atttggttta gttggttgtt caaatggtgg tgacaaagaa aaaaatggag
    30481 aaaagaaaat agcagtatta ctaccaggtt caacaggtta ctttgtagca actaaacaag
    30541 gtatagatgc aaaatctaaa gaattaggtg taagcgttga atatgcagat gctcaatggg
    30601 atgctagtaa acaattatca caagctgaag attttatggc taaaggggta gacatgattg
    30661 cattatgtgg agttgattct gctgtaagtg agagaatagt taaagctgct aatgattcag
    30721 acgttcctat agtagcattt acaaattcta taggaagtaa cccaactgga gaatttaaag
    30781 gtttagttac atatatagga caaaatgagg aagaaacagg agcattaact ggaaaaattg
    30841 ctaaaaactt attaggagaa actggtggga aagctgtatt aatagaagga gtaccaggaa
    30901 ctactccaca agtattaaga aaaaaaggtc ttgaaaaaga attaaaagat agtaatatag
    30961 aaatagttta taatcaaact tcaaattggg agaaagagca agctttaaaa gtaacagaag
    31021 atttaattca aaagaaaact gattttaatg ttataatatg tcaagatgat aattctgcta
    31081 caggtgcagg tcaagcttta aaagatgctg gtcttaagga taaagttaaa gtcataggtt
    31141 tgggtggaag taaagatgga ttaaaagcta taactgatgg gttaatagat ggtacaactt
    31201 atatgtctgc tgtagaagaa ggtggattag tcatagaaaa ggcatctaaa ttccttaaag
    31261 gtgaaaaaat agaacctgtt acacaaatta aacaagttga agttaacaaa gataatattt
    31321 cagagttcaa gggtgaatgg taaattgtca tagataataa ataagatttt tagagttact
    31381 ttagaaacta tataaagtaa ctcttttttt aatttaattt agtgataaat tattgaatag
    31441 tatttctaaa caaataaaaa attaatataa acaaagactt aattatacta aattataagt
    31501 aaaactaata taaacttatg gaaaaaagga aaacgtctaa agtattggaa ttactatagt
    31561 gtaggtgtag tattaaaaaa acataaaaaa acataaaaaa attgttgaaa aaaatataga
    31621 aattttgtat aattaaagat ttgaaaagtg cggtatacaa agccaattct tttgtatata
    31681 atatatataa attcagaaaa aggaagggat atgtatggcc aggcggttat ttttagaaag
    31741 gattggactt atatttttag gttcagcaat attagcattt ggagtttata atttttatta
    31801 tttaaacaac ataacagaag gtggagtgtt aggtatttta ctactgttaa aaaatttatt
    31861 caatatacaa ccagcaatag caaatgttgt aatagatggt ttactacttt tagtaggata
    31921 taaatttttc ggtaaaaaat tccttatata ttcaatagta gcttctataa ctttttcagt
    31981 gttatatgac ttatttgaag ctataggacc tctagtacca caatcacaaa atatgctttt
    32041 atcaactatt ttggcaggtg taactgttgg aactggtgta ggtatagtag taaaagcagg
    32101 ttgtgcatca ggaggagacg atgctttagc attagtaatt tctaagacta catctttgaa
    32161 catagggcaa gtttatttag caacagatgt tattgtattg ttgttatcat tattttatct
    32221 atcagcgttt gatatctttt attctttgat tgctgttaca attagtggca aggtaattga
    32281 ttttatttat tatcatggta aaagtttgga tatggatata agtaatgata tagttccaga
    32341 atgttaatat aaattaaata actttagatt ttattagggg tggcagattt gccacctttt
    32401 ttgtgtctaa atctttatag aaagtataat cccatctagg tttttgcaaa tctcaaatat
    32461 agaattatgt tggaatgact gggaatacga ttgctagatg tgcattttaa aaacccaatt
    32521 ttatatttat gaaatactaa tcattaatgg atgtttctta atcctatctt tatacctaaa
    32581 atgataagat aaatgaagta gcaatacaaa actaaaattt gtaaaagaga ttaaattaaa
    32641 tggaggagct aaaatgttgg atatagttat gatagcattg ttattcatag gatttgtttc
    32701 catgaagtta cttgctgatt ggtgtgggaa acaaactgaa aaaaaataat gtaatgtatg
    32761 gagggattac ctatgtggtt tctggcagtt gtaattataa tgttaataat atatttggta
    32821 tatgcgctta ttaatcctga aaaattttaa acagtataag gctaataagt aagttgaatt
    32881 ggggggaaca atttttatgg tgcagatagt aatagtgtta gcgattttta tgattttagt
    32941 aatacctatg ggcaagtatt tatatcatat agctactaat caaaaaacat ttggagatag
    33001 actatttgat aaagtggaca attttatata taaggtatgt agtatagata agaaaaaaga
    33061 aatgaactgg aaacagtatg cattagcttt attatttaca aatgcagtta tggtatttat
    33121 aggatatatc atacttagaa ctcaaagtat gcatattttt aatccaagtg gaattaaaag
    33181 tatggaacaa ggactatcat ttaatacaat aataagtttt atgacaaata caaatcttca
    33241 acattattca ggtgaatcag gactttcata ctttagtcag atgacagtga taatatatat
    33301 gatgtttaca tcagctgcaa caggatatgc ggctgctatg gcatttgtaa gaggtctagt
    33361 cggtaaaaag aaaacattgg gaaattttta tgtagattta attaggataa caacaagagt
    33421 acttttacca ggagctttaa ttattgggct tatattagtt acacaaggag taccacaaac
    33481 ttttgctggg acagaaacag tgactactat agaaggaaag cttcaagata tagctagagg
    33541 tcctgtggca gctcttgaat caataaaaca tttaggaaca aatggtggtg gattctttgg
    33601 ttcaaattca tcacacccat ttgaaaatcc aacaataata tcaaatatag tagaaatatt
    33661 atctatgatg atattaccag gagcttgtgt tgttgcattt ggtcacatga taaagaataa
    33721 aaaacaaggt tgggttgtgt ttggcgcaat gtcaatcata tttttaatag gtctagtagt
    33781 gtgctttaaa gcagaaagtg caggaaatcc tattttatca caattagggc ttaatcaaag
    33841 tatgggaagt atggaaggta aggaagttag atttggtata gcacaatcat cattattcac
    33901 aacagtgact acttccttta cgacaggtac agttaataat atgcatgata cactgactcc
    33961 tttaggagga ttagtaccac ttttaaatat gatgctaaat gtagtatttg gtggtaaagg
    34021 cgtaggtctg atgaatatgt tgatgtacgc tataatagca gtattcttat gtggattaat
    34081 ggtaggaaga acaccagagt ttttaactaa gaaaatcgaa ggaaaagaaa tgaagcttat
    34141 tgctttactt attatattac atccactttt aatactaatg ttctctggtc tttcagtagc
    34201 aataccagca gggcttgaag gaatttcaaa tcctggattc catggattat cacaggtatt
    34261 gtatgagttt gcatcttcag cagccaataa tggttcagga tttgaaggtc taggagataa
    34321 cactatgttt tggaatataa caacaggtgt agttatgttc tttggaagat atgtgtctat
    34381 tatagtacta ctagcaatat caagtttgtt agcatctaaa aaagctgtaa atgaaagtat
    34441 aggaacactt agaacggata attttacatt tacaatagtt ttagtacttg ttgttttaat
    34501 tgttggtgct ttaacatttt tcccagcctt agcgcttgga cctatttcgg agcatctagt
    34561 gttgtggcac taaaaaattg ggagggagta aagttttatg gaaactaaaa aaacaaagtt
    34621 tattacaaaa gatattttca aaacttccat tatagaagct tttaaaaaat taaatcctaa
    34681 atacatgatg aaaaatccag ttatgtttgt tgtagaaatt ggatttatag taacattgat
    34741 acttacaata gtacctagtt tgtttggtga tgaaggcaat aacttaagag cttataatgg
    34801 gattgtaaca ataatcttat ttataacagt cctttttgca aattttgctg aagctgtagc
    34861 tgaaggaaga ggtaaagctc aagcagatac acttaagaaa actagaaaag atacagtagc
    34921 aagggttata gatacgttag gaaatgaaaa aatgataagt gcaagtgagc taaaacttgg
    34981 tgatattgtt ttagtaaatg ctggagaagt tattccaaat gatggagaag ttatagaggg
    35041 tatagcttct gtggatgagt cagcaataac tggagaatct gcacctgtca tgagagaatc
    35101 aggtggagat ttcgcatctg ttacaggtgg tactacagtt gtaagtgact ggttaaagat
    35161 aagaataaca acaaaaccag gagaatcgtt cttagataaa atgatagctc ttgtagaagg
    35221 tgcttcaaga caaaaaacgc caaatgagat tgctttaaat actttattag tagggttaac
    35281 acttatattt atggttgttt taatatctct ttatccaatg gcaaactatg caggtgtaaa
    35341 gattccaatt tctactatga tagctctttt ggtatgttta ataccaacta ctataggtgg
    35401 attgttgtca gctattggta ttgcaggtat ggatagagta actagattta atgtaattgc
    35461 aatgtcagga aaagcagtag aagcttgtgg agacgttgat actatgattt tggataagac
    35521 aggtacgatt acttatggga atagacttgc atcagaattt atagcagttg gaaatgcaga
    35581 ttcaaaagat ttaataaatt attctgttat gtgttcatta aaagatgata caccagaagg
    35641 aaaatctata gttgatttag gtatgaaatt aggttcaaca ggtaagacaa aagaggcaga
    35701 ggaagctgag tttgtagaat tttctgcaca aactagaatg agtggtatta acttagctga
    35761 tggtagagct ataagaaaag gtgcttatga tagcataata aaaagagtaa aagaatctgg
    35821 tggtacagtg ccagaagatt tagaagaaaa tgtaaatcga gtagctaaac taggaggaac
    35881 acctttagta gtgtgtgtag ataatgagat atatggaatt atctacttaa aagatacagt
    35941 aaaatcaggg cttgtagaaa gatttgctag acttcgagag ataggaataa agacaataat
    36001 gtgtacaggg gataatccct tgacagcagc tacaattgca agagaagctg gagtagatgg
    36061 atttattgca gaatgtaaac cagaagataa aatagatgca ataaaaaaag aacaattaga
    36121 gggtaaaata gttgcaatga caggtgatgg aacaaatgat gctcctgcac ttgctcaggc
    36181 agatgtaggt cttgcaatga atagtggtac aacatctgca aaagaagcag caaatatggt
    36241 agatttagat tcagacccaa ctaagatatt ggaagttgtt gaaataggaa aacagctttt
    36301 aataactcgt ggggctttga caacatttag tatagcaaat gatgttgcaa aatattttgc
    36361 gattatacca gctatattta ctcttgcaat acctcaaatg gagatattaa atgtaatgaa
    36421 actttctacg tcatttagtg cgatattatc agctttaata tttaatgcta taattatccc
    36481 atgtttaata cctcttgcaa tgaagggagt aaaatataga cctatgaaat cacaagctat
    36541 gttaatgaga aatatgctta tatttggtct tggtggagtt atagtaccat ttttaggaat
    36601 aaaattaatt gatttattga ttacaccttt agtaatggtt cttaatttag gttaataaat
    36661 ggaggatata aaaatgaaaa cattaaagcc agcattactt gtaagtattg tattgttagt
    36721 tgtttgtgga cttgtatatc cacttgtttt gacaggtgta agtcaagttg catttaaaga
    36781 taaagccaat ggaagtatga tagaagtaaa tggtgtaaaa gtaggttcag aacttattgg
    36841 tcaaagcttt acagatgcga gattctttaa gggaagagta tcatcagtaa actataatac
    36901 ttatacaaaa gaagatttag taccagataa agatggaaat acatcttatg gaggtgtatc
    36961 atcagggtct tttaattatg gagctacaaa tccagaactt catgatagag tacaaaaaga
    37021 tatagaaaag tttttaaagg ataatccaac tgtcaaaaga gaggatatac caacagattt
    37081 acttacagca tcaggttcag gacttgaccc taatataagt ccagcctctg caaaaattca
    37141 gataccagct atagcaaaag cgtctggaat atcagaaagt aaattacaaa aaattgtaga
    37201 tgataatact tccaaaaaac tttttggagt tttaggtgaa gatagagtta atgtattaaa
    37261 agttaatgta gaggtcgcaa aaatattagg tcttatatag tattttgtgg aattttagca
    37321 acaaaatatt acgcaagatt taatttaaag gttaaatttt aaagagtgaa aatcgataat
    37381 gattttcact tttttatttg aatattacta atatttggaa taaagtgatt gtaagtataa
    37441 aatccaaaaa aaggttgcat tatcaaaatg ataatactat aatatattta tcgttatcaa
    37501 tttgataata gatttggaca tataggagga aatagtatgt tatataataa cgcattagag
    37561 ttgataggaa acacacctgt agtaaggctg aataatttag gctgtactaa tgggtgttct
    37621 aacatatatg taaaattaga aaaagtaaat ccagcaggaa gtataaaaga tagagctgtt
    37681 tatgaaatgt tagagggctt agagcaaaga ggagaattaa aaaagggaga tgtactagtt
    37741 gaagcaacta gtggaaatac tggtattgca ttatctatga taggaaaatt aaaaggatat
    37801 gaagtagtaa tcgtaatgcc cgaaacaatg agcgttgaga gaagagattt aatgaaggct
    37861 tatggagcaa atttggtgct tacagatggt aaagctggaa tggcaggttc catagaaaaa
    37921 gcaaatgaac ttttaaaaga aaatccaaac tataaaagcc taaaacaatt tgaaaataaa
    37981 gataatccaa atgcacatta taaaacaaca gcagttgaaa ttatgaatga tgtcaaagat
    38041 ttagatatat tcgtatgtgg agttggaaca ggtggtactt taataggaac tgcaagatat
    38101 ttaaaagaac aaaatccaaa tataagagta attgcacttg aaccaaaaaa atctccagct
    38161 atatcagaaa acaagactgg acctcataaa atacaaggaa taggtgctaa ctttgttcct
    38221 gaaaattatg attctagtgt agttgatgaa gtaatattag tagatgatga agatgcattt
    38281 gatacagtta aattacttgc agcaaaagag ggaattttag tagggatatc ttctggagca
    38341 aatatatttg gtgctataga gatggcaaaa aagtatccag ataaaaagat agttacagta
    38401 gctccagatg gagttgataa gtatatgtct atgggaatat tttaataatt ttatttgagg
    38461 gagtgaatac tttgtttaag aaaataaata aagatattga atatataatg aagaatgatc
    38521 cagcagctag gtctaagata gaagtttttt tactttatcc atcagttcat gcaatgataa
    38581 tgcataggat ggctcatgct ctttataaga agaagaagct ttttacagca agattaatat
    38641 cccagatatc cagatttatg actggcatag aaatacatcc tggtgctaaa atgggggaag
    38701 gtatattgat agaccatgga atgggtgttg taattggaga gacagctgaa gtagggaata
    38761 gggtgacaat atatcaagga gctactttag gagctacagg aaaagatact ggaaagagac
    38821 atccaactgt tggtgatgat gtattaattg gtgctggaac aaaaatacta ggacctttaa
    38881 atataggttc aaattcaaaa attggtgcaa attctgttgt cgttaaggat gtacctaatg
    38941 gtgcaacagt tgttggtata ccagcaaaaa tagtaaaaat aagaaaccta gaacctgtaa
    39001 aaaagaataa aaaagaagtg tcctatgagt atgatgaatt agataatgta tattatatat
    39061 aggatttata taactataaa ataaacttta taaaaattat tttaaaaggt atttttttca
    39121 aatttgagga cataataaaa aagaactgaa tgttacagtt ttggttcatc aatctttgat
    39181 aaacaagatt ttaaatttgt agcgtctagg aggattttat tatgaaagca aatgttaatc
    39241 aagatacttg tataggttgt ggattatgtc catcaatatg tccagaagta tttgatatga
    39301 aggatgatgg gaaatcacat gttatagttg atgaagtacc aagtgatgct gaagaatcag
    39361 cagcagaagc tagagaaagc tgtccagttg atgcaataga tgtgcattaa taaaataaat
    39421 gcctataaaa attgaatttt caatttttat aggcatttgt cttttattca gttagtattt
    39481 caacacctgt ttctgtaact aaaaccatac attcccattg agcagataat ttaccatctt
    39541 ttgtataaat agtccaatta ttttcttcat caacaaaaat ttcgtgagtt ccttcattta
    39601 ccataggttc tattgtgaat atcatcccag gaaccaatag catctcagtc ccttttttag
    39661 atacataact tacaaaaggg tcttcatgaa agcttaatcc aactccatga ccacctattt
    39721 ctctaacaac agagtatcca tttttctttg cataagagtt gatagcatca cctatatctc
    39781 ctaaaaatcc ccatggtttg gcaacttcaa gacctttttg gacacattcc tttgtaactt
    39841 gaactaattt ttttgcattt tcattaacat tacctatcat aaacattctt gaagcatctg
    39901 aatagtatcc attaaaaata gtagaaacat ctacatttat aatatctcca tcttttaaga
    39961 taatgtcttt acttggaata ccatgacaaa cttcattatt tactgatgta catacacttt
    40021 taggaaatcc attatagtct agtggagcag ggatagctcc attttgtagt gtaaagtcat
    40081 gaaccaactt atttatatct tctgtagaca ttccaatttc gattttatct gaaatataat
    40141 ccaaaatttt tgtgtttatt acagcacttt tttttatttc ctcaatttga ctttcatttt
    40201 ttataatatc atgagttggt acaatatgac ctaacttagc aaatgaatct attttattat
    40261 ccatatccat atgacatttt ttatattttt tagtacttcc acaccaacag ctatcatttc
    40321 tatttaactt cataactttt catccttctt ttattattta tttaaattat gggttacata
    40381 aactatttta aaacataacc attataaata ttataacata aatactaaga aatcctttat
    40441 agatagtgat ttattcaaag tataaattat tatatggtta ttgttgatga aaagaggata
    40501 gttaaatgta ttattggata ttagcgcact taatagttta gttcaagttt ttcaagaaaa
    40561 tcatatgtta gaggacaatt ttcctttaaa gtattttttg aatcttcatc aagataataa
    40621 tgtacaaatg attctgcaaa aaattcttct ataaacttgc ttatatatgc atgtgcaact
    40681 tgcttagggt cataaagctt atatctttcc tgagcaaata cttccataaa ttcttcagaa
    40741 tttgattttt tatttaaaac atttttatct atagcatgtg cagtttcatg taattctaaa
    40801 ttttttgatg aatgtacatt tgcatagcta gggttactat aacctatttt agccactata
    40861 gaattatttc tatatatacc aggtacatct ttccaagtct tgcctgatct tacccaacat
    40921 ggaggtaatt gatattttaa atcactaaat tctggttcat cagttagatt tgaatttatt
    40981 aattttattt ttatatcctt ttctttaagt gtctgtaaat atttaatatt tatttttgca
    41041 agtctgtttt tcatattttc aacttcatct ttatcataat tcaagtcatc aggaagtata
    41101 agcatttgtt caattatatc tatactactg tcttttatat tttcagattc ttcatcaaac
    41161 ttatattgaa gagtattatt ttcatagata gttatatttg tattattttc tatagaataa
    41221 gagtgaatat cctctaaatc taaaatattc atttgttgta aaatatgttt tgtttcgcca
    41281 ctccatacta ctaaagaaag tgaattaaaa ttaattagtg gattttcttt tgcttttata
    41341 tcattgctta tatctacatc aaatacatat tcaattgagt cttctatgtt aagaccatct
    41401 gtattctttt taatatataa atacgcttgt tttaagtcta tataaatctc atctaaagat
    41461 tcgtcataaa attctgataa attattagta aagagttctt cacttaaaag ttcattttta
    41521 tcagttatac tttcaattaa aatattaatt tcttttatca tatcgtaaat acttattttt
    41581 ctatttgctt tttcactaga tacattgcat ggcgcaagag caatgcaata aaaatctttt
    41641 tcttttttat aaaaggcttt aagtgatttt gataaaagtg taaagtctac acctatctgt
    41701 aagttgacct taagtgagtc attttgtaat aattcagtat tatttctttt agctgtattt
    41761 aaattgtttt ccataatcat tgcccccttt ttattagggt atcataattt ttttatatgt
    41821 gtcaatttgt ttttttgaaa attataattt ttttgaaaat tttatttttt ataatttgtg
    41881 aattttttat tatagaaata aatatgttat tattatgtca aaatttagca ttagattcat
    41941 gtagagtatg ttttatacat atattataat taattctttg aagattgcat attcaaaata
    42001 cacaccattt tttttaattc tataatataa tatttatata tttaatgtta ggatgtgatt
    42061 taaggtgtat tttacatata ttataagatg taaagatgat tctctataca ctggatatac
    42121 cagtaatata gttagaagaa tgaatgaaca taaattagga attaattcaa aatacacaag
    42181 agcaaaggga tttgaaaaac tagaagttta ttttgtgacc aacacaaaaa gcaatgctat
    42241 gaagttagaa tactatataa aaaaattaac tagaaataaa aaattgtcta taataaaaaa
    42301 cccaagtata cttataaatt taattgataa taaagaagat tatattattg gtaaggaaat
    42361 agaacaatta acataaatat ttatagtaac attataatgg gtgcatatgg tgtaaaaact
    42421 tgacaataaa tactattgta atataatgaa gttataaata tagtaaatta aaatagctag
    42481 gggagccaag agctgagagg tataccgacc cttacacctg atctggataa taccagcgta
    42541 ggaaagctta gtaaaaattc aagtatgtat tatttgtgat atatgctatt tctaagctat
    42601 acctattgta ggtatagctt ttttaattaa gttatttaaa ttaaacttta ggaggcaaca
    42661 atgagtaatt ataaaatacc aacacttaca atagccggct ctgattcatc tggaggagct
    42721 gggattcagg ctgatttgaa aacgtttagt gcaattggta cttatggtat gagtgtaata
    42781 acagctataa cagcacagaa tactaaagga gtttttgctg tagaggattt aaataagaaa
    42841 ataataaaaa aacaaataga ggcagtcttt gaagatatac ctccaagagc tgtaaaaata
    42901 ggaatggttt caagtcctga gattatactt gaaatagttg agaatctaaa gaaatacaat
    42961 ccaaaatatt tggttgtgga cccagtaatg atatctaaaa gtggatatta tctattaaag
    43021 ccagaggcaa aagaaaactt aattaagtat ttgataccac ttgcatacat aattacacca
    43081 aatataccag aggcagaaga aataacagga ataaaaatac ataatgtaga tgatatgaag
    43141 agagtaggag aagaaatact acaacttgga cctaaatttg tacttatgaa aggaggtcat
    43201 ttagatggag aagcagtgga tattttagtt ggtaaaaata tatttaaagt ttataaaagt
    43261 gaaagaattg ataaaaaaaa tacacatgga acagggtgca cattatcttc tgcaataaca
    43321 tcttatctag cattaggata tgaaataaca gaagcagtaa atctaagtaa aatctatata
    43381 acagaagcaa taaaaagaag ttttgatata ggtcatggag taggaccagt acatcatttt
    43441 tataaatttg agtaataaaa aaatattgga ggtagaatat gtataattta attaaagatg
    43501 taaaaaaatt aaatccattg gtaattcatt atactaataa tgtaactata aatgattgtg
    43561 ctaatgtaac tttagctgta ggagcaagtc ctcttatgag tttttcatat gaggaagtag
    43621 aagaaatggt aagtgtagca aactctgtag taataaatat aggaactatg aactcaaata
    43681 tgttggattt attcctatta gctggaaaag cagcaaataa gtacaataaa cctgttgttt
    43741 tggacccagt tggagtattt gcaagcaaag cgagagcaga acttacaagt agacttttaa
    43801 atgaagtcaa atttagtgtt gttaaaggga atgtatcaga aataaagttt ataggtggct
    43861 ttaatgtaag gggtaaaggt gtagattcat ttgatgaaga agaagattct actgagataa
    43921 taagaaagat tgctgaaaag cttgaatgtg tagttgtagc aactggaaaa atagatataa
    43981 taactaatgg taaaggtaca tataaaataa acaatggaac tgataaatta aaaggaatta
    44041 caggaactgg atgtatgact gctagcttga ttgcaagttt tatggctgtt actgagaata
    44101 tattagaagc agcaacaatg ggtgttctta caatgtcttt aagtggggaa ttagctaatt
    44161 taaataatcc accaataggt acatttaaag aaaatcttat gaatgctatt tatcaaatgg
    44221 atatagatgc tttaagtaaa aattctaata ttgaattttt aaattaataa agagtaaggg
    44281 agacttaaaa tgattgataa agaatcttta aaaaaatgtt taaagttgta tttagtaact
    44341 gactctgaaa tgcttaaagg tagagacttt tataaatgtc ttgaagatgc aatatcttct
    44401 ggaataacaa ctgtacaact tagagaaaaa aatgcttctg gaagagagtt tttaagaaaa
    44461 gctatgaaac ttagagaaat aactaaaaga tatggtgtta agtttataat aaatgataga
    44521 gtggatatag cacttatttg tgatgcagat ggagtacatg taggacagag tgacattgat
    44581 gttagagagg ttagaaagct tataggaaac aataaaattc taggagtatc agcaagaact
    44641 ttagaagaag ctatttgtgc aaaaaatgat ggcgcagact atttaggtgt agggtctata
    44701 ttcacaacat ctactaaatt agatgcaaaa tcagcttcgt ttgaaactgt taaagaaatt
    44761 aaagaaaaag tagacatgcc atttgtacta attggaggta taaatttaga caatatagat
    44821 aaacttaaat gtttagaaag tgacgggtat gctataatat cagctatatt aaaagctgaa
    44881 gatatctcta aagaagtgga aaagtggaca ttaaaaatat aaaattggct ctggtagtga
    44941 aaaaaatgga aaaatttgat ataatctaag agtaaaattt aagcaattat aaatgataaa
    45001 cttttctaca tatacttttt aatcaagata tatgtagatt tggttaaaaa ggagctataa
    45061 atgattagga tattgttagt gtgtgttgga ggcatgtctt ctaccctatt ggtaaataaa
    45121 atggagaagg atgcaaaaaa aagaaatata gactgtaaaa tatgggcagt tggagaagga
    45181 gatataaaat cggagcttga taactttgat attttacttc ttggaccaca acttagattt
    45241 atgcttgatg acgtaaagtc tatagttgga gatagagctc cagtatctat aatagatatg
    45301 gtaaactatg gcacttgtaa tggtcatgct gtgttaaatt cggttttaga aatattaaaa
    45361 taaataataa aagtggattc tatataatag agtccatttt tattatttgt tattaatagg
    45421 cttaaaactt agcttaccat ttcttttttt aatctccaca tagcaagtga aaaacttgaa
    45481 cagcacaata ttaaaaatag taggataaca ggcattatat ctccaataag aatagtctca
    45541 ccaaatatta agctacgtaa agcgttaata acatgggtaa atggattgag catcactgct
    45601 acttttaatc ctccagataa actttctata gggaaaagag ctgaacttaa aaagaaaata
    45661 ggaagtacaa ttgcagtcat aattgtttca taaactattt catttggcaa taataaactt
    45721 atagaataag ctagacttga taaaaagaag gctgtcataa atatcagtac tatcataagt
    45781 aggattccac caatgccaga ttctatcctt actgagaaga atatacttac tatacacaaa
    45841 atagttactt ctatgaaaga aactaagata gcttctaata attgaccaag gacgattgaa
    45901 tacctgctga taggtgcaat taagactcta tagaaacttc cactgttctt cattataaag
    45961 tttataatac caccactact acaagaagaa aacacaacca gtacaatgat acctggtagt
    46021 ataaaagcag tataattatt gatatttata ttattcatag tttggtttgc aatagaacta
    46081 taaagcacaa gccaaagaaa aggttgtaat atagtaacta caaatgagat tggattttta
    46141 aaacgccact tcatactacg ccataagata tttaacattc ccattcctcc tttaagcttt
    46201 ttttagaact tgtaagtgct aaaaatacat cttcgagact tggctctaca atttctattg
    46261 catcaaattc tatatcatgt tctaaaagcc atttatttat taaagtaaaa gcagttctac
    46321 tatcgtttac acttataaaa attgaatttt ctcgtacgct cataaacttt actaaaccag
    46381 tacttttaat tgaatcttta tattttttta tatcttcagt attatgaaaa gttatacgta
    46441 gtatattttg tctaatgtaa ctacgtaaac ttgatggtgt tccttgtgct aaatcctttc
    46501 cattcttcat aatacaaata ttatcactca attgttcagc ttcttcaaga taatgagtag
    46561 ttagaaatat agttgtgcca tactcatctc taatttttaa taacatatcc cacatagatt
    46621 ttcttgaatc gacatccata ccaacagttg gttcatctaa aaataggatt ttaggagatg
    46681 ataccatatt catagctatg tccaatctac gttttacacc ccctgaatat gatgaagtag
    46741 gatattttaa gtaactagat aagtcaaact tatcaattaa agagtctatt ctttgttttg
    46801 caatctgtgg ttctacttta tacattttac tttgaaatac catattttcc ataagggata
    46861 aatgttcatc aatagagatt tgctgtgcta cacaagcaat ttgagtacgt atccaagaag
    46921 gattatctac taaatctttt ccaaacattg ttacatttcc agatgtaggt ttgtaaaagg
    46981 tagtcaaaat atttataaga gaagatttac cagcaccatt aggaccaagt aaagaaaaaa
    47041 tttctccagc atttacatta aagcttagac catttaaagc tttcacacca tttttatatt
    47101 cttttactaa attgtctatt tcaatcgcta acatatatta ttcctcctct ttaagtggaa
    47161 atattatctc tgtaatatag ttatctgggt ttccttttat caacatacct ggtcctttaa
    47221 taaatacttc tctccaagga gtttgtattt ttatattatt ttcatgaata tatttaaata
    47281 tcatttcata cgtttttggc aaattactat aagagcctgt gtgagtaaaa aataaagctt
    47341 ttgttttagg aaattctttt gtagttatac ccatacgatt tggagtttca gcagttggaa
    47401 cacataattc catttctgca acaccagttt tttggtcaac atcatagtag caaaagaatg
    47461 gtgcaccgtt agactttcct tttatagctt taaatacatt tggaaaatac tttgttgcct
    47521 cagtcatagg tccattataa cgcatggtag ccactgtgac tgattcgata tttttgattt
    47581 caattttgca attcatttgt ttacctccta atgtgtgttc atattcttac tagattttta
    47641 agcattaata catataagat tttgattagt tgaaaacata taatcttatc tcgtttgctt
    47701 gttttcaagt ctaacataac ataaaatata tatcctctca tttgatgctc cattttgtaa
    47761 agttattatt taagctttac tttagatttt ttagcaaatt aaaataaagc aatttataaa
    47821 ataagtattt acaaattaag tgtattaatc gtataataca cttaatagga tactagataa
    47881 agtctgaaat aaaggcgtat ttttttaaag taagtgtact atcgtattaa tacactagaa
    47941 taggaggaat catgaatttt gaattagata atacaactcc catttatttg caaatagtaa
    48001 agtatataaa gagacaaata gtaacaggag agttgaaacc aggagagaca ataccatcaa
    48061 gaagagaaat ggcacttaat ctaaaagtaa acttaaatac agttcaaaga gcatataagg
    48121 agatgggtga tatgaatata ataaatactt ttaaaaatta tcaaagcagt gtaacagttg
    48181 atgaaaatat attaaaaaat ttaaaattag agcttataaa tgaatcacta tctgttttta
    48241 tagaagatat gaaagcaata aatgttagca aagaagaagt tttaaaaata atagaagata
    48301 agtattagtt ttttaacaaa tagaaaactt agtttagaaa gtttatttat aagcatattg
    48361 gatgtggagg atagtaaata tgatagaaat aaagaatgtc agcaaaacat ataaaaggat
    48421 gcaagggctt aaaataagaa agatagaagc tttaaaaaat gtaagtttta atatagaaaa
    48481 gggaaaaata actgcattac ttggtataaa tggtgttggt aaatcaacta tgttaaaagc
    48541 aatagcagga cttataaaca ttgatagtgg agaaattcgt atagatggag aaaaaataaa
    48601 tgaaaaggta tacaataaat tggcttttgt tccagatgta caatctcact ttagcaatac
    48661 aacaataaaa gaaacatttg aatttatgga aattttttat tcaaagtgga ataaagaaaa
    48721 atcaaaagaa atgatggaca tatttaaatt ggatgaagat gaaataattg ataacttgtc
    48781 aaaaggaaac attgcaagag taaaattaat acttggattt tgccaagacc cagagtacat
    48841 acttcttgat gaaccattta caggaattga tttgtttaag agggaagagt ttataggagt
    48901 aatagcgcaa tatatggagg aaaatcaagc cattattata actactcatg agatagtaga
    48961 gatagaaagt ttagttgatg aggttgtaat tcttgatgaa gggcagatta taacttcatt
    49021 taatgcagaa gaattaagag aaagagaagg taaatctata ttagataaga tgagggaggt
    49081 atataagaat gaataaactt ctatatctaa taaatgtaga tttgagaaga agcaataagt
    49141 tttatatagc ttatatatca tttttttcat taattacact tagtttaaat ttattcgaaa
    49201 ttaataaatt taaaaattct aaatttatta tgaacttagc aatagaagat tttggaggta
    49261 tattttatgg actagggata cttagtaatc ttggttttat ccaaagtata ttgttctttg
    49321 ggatattggg actttttata tactatattt ttatgtggaa aagagagttc ttttcaaata
    49381 atcaaagtat ttatacactt atgatgttac ctcaaaataa gtttaagatt tgtatatcta
    49441 aatcaatagc cttgataaca atgatatatg gttttttgat aagtcagaca gcaacattat
    49501 ttatatgtaa atatatattt aatttcattt ttagaaatat gcctataatc aatatgaact
    49561 ttgccaaaga tttatattat ttaaacttga agagtatacc aattgatttt atctctttta
    49621 tggctattta tgtattttta ctacttatag ctatttctat tgtaaattgc tgtatattat
    49681 ttatcttttc ctatataaat aaatttta
//
LOCUS       DAMJYR010000014        49608 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.014, whole genome shotgun sequence.
ACCESSION   DAMJYR010000014 DAMJYR010000000
VERSION     DAMJYR010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 49608)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49608)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49608
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.014"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            109..759
                     /locus_tag="PC471_000787"
     CDS             109..759
                     /locus_tag="PC471_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HDF2787927.1"
                     /translation="MKAEKTSIYPIIKNNLNKISIVEYKKGQRFVASDKDLQEVFFII
                     EGVALVECTTRGGNKFLVDIVPENEFIGKISYIYEHNLKCDIFAKTNIKLFRFEKNVF
                     EEFYLKPDFIALFHRKCTRRIYELYKTRMVRELFSCTEVIAYCISNKQENNVCNIKAI
                     RNFSEIYSISRKSRYHSLLKLTEKEIVKKMGNTYEILNYDELHKLAFEVKEFLEDE"
     gene            complement(899..2275)
                     /locus_tag="PC471_000788"
     CDS             complement(899..2275)
                     /locus_tag="PC471_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HDF2787928.1"
                     /translation="MQELFSLKDENKRFYKILLSLCIPIIIQNLISTSVNVIDTIMIS
                     SLGETSVASVGVANQFFFLFNMSLSGITGGAGVFISQFYGKKDVSNIRKVTGLTCVLA
                     IVLSFVFVIPALLTPKPIIHIFSYDSEVVKLCIDYFSIAVFSYPLIAVSTVFSTGSRG
                     VRNPKLGMICSAFALVTNVILNYGFIFGNFGLPALGVKGAALATVIARICELILMITY
                     VYLYKKDYILKFGLKNLKAIDKIFIKSFSSKSFPIFVNDSVWAIGTVLYSVAYARAGT
                     SAIAASQIATSTGNFFIMTAVCIASGASIMLGNELGADHIKRAIEYAKKFSILVFSAG
                     LILGIILILNIPLLLKMFSVSDSLASDITKIFFIMGILMALKSFNTLVIIGILRSGGD
                     TKYALFLELGCMWLASIPLTFIAAFKGAPIFVLVLLTYSEEVVKFIFGVPRALSKKWA
                     INIVKEID"
     gene            complement(2291..4204)
                     /locus_tag="PC471_000789"
     CDS             complement(2291..4204)
                     /locus_tag="PC471_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HDF2787929.1"
                     /translation="MIVLSCNNLNKSFGIDSILENISFTVNEGDKIGIIGVNGTGKTT
                     LFKIISGIYGYDSGDIYTSKDCEIGYLEQNTNFYSDNTILEEVLEVFKNLIEMESYLR
                     ELEVKISEESTKTNSPIIEKIMDEYSHKLELFSDLNGYGYKSEAKGVLKGLGFSDNDM
                     DKPISILSGGEKTRVLLGKLLLKKPTLLLLDEPTNHLDSEAIEWLEVFLKQYKGTVML
                     ISHDRYFLDQSVNRIFEVHNKKLKVYNGNYSKFVELSKIEKELELKKFEDQQKEIKKQ
                     EESIERLKAYGREKHLKRARSKEKALDKVDVLDKPEAYRKKARIQFTPSVQSGNDVLQ
                     IRDVSMGYGERILFKDLDLDIYRGEKVALIGANGVGKSTLFKIITNELQPLSGNIKFG
                     TNVHVSYFHQEQKTLNLDNTIIDEIWENNTHLTQTTLRNMLGAFLFVDEEVFKKISTL
                     SGGERARVAILKLILSNANLLLLDEPTNHLDIDSKEVLEEALTNYDGTIFTISHDRYF
                     LNTVVDKILVLDENGITEYLGNYDYYIDKKRQIQEMSIIEEKEEKTRTQIKDEKRKER
                     EQREIEKKNRIKRQNIEKEIEKLEIEIEKLDILLCQEEVYSNPDKAKEVSQEKINLEN
                     NLASLYDEWEEFM"
     gene            4340..4972
                     /locus_tag="PC471_000790"
     CDS             4340..4972
                     /locus_tag="PC471_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HDF2787930.1"
                     /translation="MLGNKNISMAVIRRLPKYHRYLGDLLDRDIQRISSKELSDIIGF
                     TASQIRQDLNNFGGFGQQGYGYNVEALHTEIGKILGLDRPYNAVLVGAGNLGQAIANY
                     AGFRKAGFEIKALFDANPRMIGLKIREFEVLDSDTLEDFIKNNNIDIAVLCIPKNGAQ
                     EVINRVVKAGIKGVWNFAPLDLEVPKGVIVENVNLTESLFTLSYLMKEGK"
     gene            5111..5650
                     /locus_tag="PC471_000791"
     CDS             5111..5650
                     /locus_tag="PC471_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HDF2787931.1"
                     /translation="MVFVEELWKKAIEFHGHECPGLAIGFKAALAAREYLGCKQSGDE
                     EIVCISENDACGIDGIQVVLSCTMGKGNLLLRMTGKSVYHFFNRENGKSIRIYVKSKP
                     FQMDREGYKKYLLESDFEEIFEITDTKLRLPEKARIFKSLKCEVCGEFASEQYIRIEE
                     GKKVCLDCFNKYERFYEEY"
     gene            complement(5826..5996)
                     /locus_tag="PC471_000792"
     CDS             complement(5826..5996)
                     /locus_tag="PC471_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004619190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HDF2787932.1"
                     /translation="MAYKITDACISCGACEAECPVSCISAGDDAYVIDAGSCIDCGSC
                     AGACPVDAPQPE"
     gene            6281..7348
                     /locus_tag="PC471_000793"
     CDS             6281..7348
                     /locus_tag="PC471_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HDF2787933.1"
                     /translation="MKNKLVYICIMSLLLFVVGCSNNPNKESTIKKDSKNNQSQVKEE
                     KREPVKKDITISFAGDVTMGNYKGSSYYGSFDHEFKRQGEDYNYFLKNVKPVFEKDDL
                     TVVNLEGPLTTASNAKEKKFAFKGKPGYVNILKSGSVEAVSIANNHSEDYFEEGMKDT
                     QFILDENKINYFGLGKDAVVDVKGIKVGLLGYNGLSTEYNEKNLKQMEDDIKSLKKKS
                     DVVVVYFHWGIELDYYPDKKQKDFAHYAIDKGADLVMGSHPHVIQGIEKYKNKYIAYS
                     LGNFCFGGNKNPSDTDSYIYQQTFTFSDNKLTSIKEPNIIPTSITSSNSRNNYQPKIL
                     DGSEKDRVLNKLEKISKDLNK"
     gene            7518..9419
                     /gene="cooS"
                     /locus_tag="PC471_000794"
     CDS             7518..9419
                     /gene="cooS"
                     /locus_tag="PC471_000794"
                     /EC_number="1.2.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic carbon-monoxide dehydrogenase
                     catalytic subunit"
                     /protein_id="HDF2787934.1"
                     /translation="MACNTCKMCESADKKLESFVASKDVETAFHRTEDQKVKCGFGLQ
                     GVCCRLCSNGPCRVTPKSPRGICGADADTIVARNFLRAVASGAACYLHVVENTAKNLK
                     NVGITKGVVKGEKTLNQLAEMFGIESDEKYDKCIKVADKVINDLYKSRDDKMELVEKI
                     AYAPRVKKWKELGIMPGGAKSEVFDAIVKSSTNLNSDPVDMLVNCLNLGISTGLYGLT
                     LTNLLNDVMLGEPVIRMAPVGFNVIDPDYINIMITGHQHSTFANFQDRLKDEDVIKLA
                     QSVGAKGFKLVGCTCVGQDLQLRGEHYQEVFAGHAGNNFTSEAVLSTGAIDIVLSEFN
                     CTIPGLEPIADKYKVKMVCLDDVAKKANAEYIGLDRSKLDELSNTLIEKALESYKERR
                     GSIEIDIPKDHGFEQSLTGVSEKNLKAFLGDSWKPLINLIAEGKIKGVAAVVGCSNMT
                     AGGHDVNTVELTKELIKKDIIVLSAGCSTGGLENVGLMSPGAEELAGENLKEVCKTLG
                     IPPVLNFGPCLAIGRLEIVATELAAELGIDLPQLPLVLSAPQWLEEQALADGAFGLAL
                     GLPLHLALPPFITGGKLVTEVLTEKLKDLTGGHVIVNPDPKSSANQLEEIIIDRRKNL
                     GLKDVECNA"
     gene            9412..9885
                     /locus_tag="PC471_000795"
     CDS             9412..9885
                     /locus_tag="PC471_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HDF2787935.1"
                     /translation="MHRIFINKDLCTGCKSCVLACMLKHNKDYDMYTLDLENIDNDSR
                     GHIELDSKHNNPVPILCRHCDEPECVLACMSGAMHKDSESGIVSYDEEKCGSCYMCVM
                     SCPYGLLKPDDRSKQNILKCDLCKDEEYPRCVANCPSGAIELQKEENDELCSVRG"
     gene            9860..11086
                     /locus_tag="PC471_000796"
     CDS             9860..11086
                     /locus_tag="PC471_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HDF2787936.1"
                     /translation="MNYVVLGASAAGINAVKTLRELDKDSNIVVISKDENVYSRCMLH
                     HVISEHRILKQINFVDEDFMEQNNVKWIAGKTVKGIDINKKVVQTEDITVNYDKLLIA
                     TGASSAIPPIKNLRDGNFVYSVRNIDDIYKIKEKAQNSKNVVIIGAGLVGIDALVGLF
                     KYEQLNISVAFMEKYILDRQLDEYTASVYENKFKEKGVKFYPSASIQEIVLDNSKNVT
                     GVAFSNGEVLDADMVIVATGVKPNADFLDGTGIEYDRGIIIDDMCQTTQKDIYAAGDV
                     VGKNAIWPLAVKQGIVAAYNMVGKDKKIEDEFAFKNSMNFMDIPTISIGMNTPVDDSY
                     KVLTRHGINDYKKFVFKDNVIYGAVIQGDISYVGVLTYLIKNKVEIYDLENRIFDICY
                     ADFFNIKENGEFCYSV"
     gene            complement(11225..12412)
                     /locus_tag="PC471_000797"
     CDS             complement(11225..12412)
                     /locus_tag="PC471_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDF2787937.1"
                     /translation="MNITKNFLKDYLEKFGETPFLVRLKDEEEFLIGEGSPQFKVIVN
                     NDISKSELLKSTSLALGEAYINRDIEVEGDLFLTLNLLLKQIDKFNIDNSAVKHLLHS
                     SNSMRHQKKEIHAHYDIGNDFYSLWLDKTLSYSCAYFRNDNDTLYDAQVNKVHHILTK
                     LNLEEGMSLLDIGCGWGFLLIEAAKKYKIHGVGITLSEEQFKEFNARIKEENLQDYLE
                     VRLMDYRELKKSDLMFDRIVSVGMLEHVGRENYELFIKNVNAVLKDGGLFLLHYISGL
                     QESYGDPWIKKYIFPGGVIPSLREMIHICSDYKYHTIDVESLRLHYVKTLLYWQKNFS
                     ENLPKIKEMFDDKFIRMWDLYLCSCAAAFNTGIIDIHQILFTKGIKNDLPLTREYMYN
                     CSK"
     gene            12685..13509
                     /locus_tag="PC471_000798"
     CDS             12685..13509
                     /locus_tag="PC471_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDF2787938.1"
                     /translation="MEVYALVGSSGTGKSFKALEFAYENDIEYIIDDGILIYKNKVLA
                     GISAKQANTTIEAVKRAIFYNLEHRQEVREAIKKENIRKILIIGTSKKMVNQIVERLS
                     IGRVCKFINIQDISTKYEIEIAKQARKEGNHVIPVPAVEIKSMASGLSINSLKRLFRK
                     GSNRNMVVLEKTIIRPTFSYIGKFYISADVIKQIIEYEVYNFGSIDRINKINIENCNN
                     FMNIFVSININDLETIKKIEDIQQTIKKSIEKMTLVNVQKIDINIHKIKKINFLQC"
     gene            13638..14903
                     /gene="gluD"
                     /locus_tag="PC471_000799"
     CDS             13638..14903
                     /gene="gluD"
                     /locus_tag="PC471_000799"
                     /EC_number="1.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-specific glutamate dehydrogenase"
                     /protein_id="HDF2787939.1"
                     /translation="MSGKDVNVFEMAQSQVKNACDKLGMEPAVYELLKEPMRVIEVSI
                     PVKMDDGSIKTFKGFRSQHNDAVGPTKGGIRFHQNVSRDEVKALSIWMTFKCSVTGIP
                     YGGGKGGIIVDPSTLSQGELERLSRGYIDGIYKLIGEKVDVPAPDVNTNGQIMSWMVD
                     EYNKLTGQSSIGVITGKPVEFGGSLGRTAATGFGVAVTAREAAAKLGIDMKKAKIAVQ
                     GIGNVGSYTVLNCEKLGGTVVAMAEWCKSEGSYAIYNENGLDGQAMLDYMKEHGNLLN
                     FPGAKRISLEEFWASDVDIVIPAALENSITKEVAESIKAKLVCEAANGPTTPEADEVF
                     AERGIVLTPDILTNAGGVTVSYFEWVQNLYGYYWSEEEVEQKEEIAMVKAFESIWKIK
                     EEYNVTMREAAYMHSIKKVAEAMKLRGWY"
     gene            15116..15706
                     /locus_tag="PC471_000800"
     CDS             15116..15706
                     /locus_tag="PC471_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HDF2787940.1"
                     /translation="MHFKSKDLNLKYLVLFLKIVYWFNPFIYIMEKYMDLDCELYCDE
                     RVLKNCTVQQKQEYALTIVNSMKRSTNFSNKFVAGLNKQSDIRKRVINMFSEKHKNGI
                     LRALIISLFSSITFFKFDSTSIIEENDFLERSRYTFEGRQEDMFVTIDFIYADVPIKL
                     RKEHEENCRVNGIVPKDSDSIEIIPRFYKDCMDLKK"
     gene            16037..17170
                     /locus_tag="PC471_000801"
     CDS             16037..17170
                     /locus_tag="PC471_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M56"
                     /protein_id="HDF2787941.1"
                     /translation="MSNFILNGIFRTVIVSSLSILMILIFKKNVFKRFSKKFNYYIWM
                     IVVIKLFLPFTYYTFTINILRSKKHININNINLEGFNNVSTISNNIILYTWIITVVVY
                     LIYTIFKYIKLKNLINDLSYDVDDGEIVNLYKNILEEFNITKDIKLKYSYEVETPAFF
                     NSCVLLPPHEYKLKELDWIFRHELMHFKSRDLYLKYVILFLKTVYWFNPFIYIMDKHI
                     DLDCELYCDERVLKNRNSEEKKDYALTILNAMRKGSNTSNKFIAGLHKQSDIKKRVMN
                     MFNEKYKNGILMALTLCLLSSITFLKVNSTSIVNLNILPKGMEEKDVTNSAIVTMIEF
                     TYAEAPKSMREEHKINCEFLGLIPKDSDKIGVGIEYYDSLLND"
     gene            18086..19108
                     /locus_tag="PC471_000802"
     CDS             18086..19108
                     /locus_tag="PC471_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HDF2787942.1"
                     /translation="MKKKILIPVFASVMALSVSSIVNADEVNDSSQNKDDKTNTELNL
                     GEYKEVKYKVAKIKDGVAIKIREEGQVQNIAYSGDEFTVLGTQGEWVKVKVEDGEGWL
                     ATRYVDISEGVGYTNADKVNLRKDKSESSEVIEELEKGSSLLVLEDNGDWLKVKDGET
                     EGYVKSSYISDKAPVIEEPQVNPSIDQNVDSTNNNQQNNANTNQQNNNSNVPTANSNA
                     VQAVLNLAYSKQGCPYVWGAEGPNTFDCSGFTQYVYRNAVGKNIPRTSKAQSKYGQTV
                     SKANLQPGDLVFFTTNGSGSVSHVGIYVGGGNMIHSPSTGKTVSVTSINSSYYTARFV
                     TAKRIL"
     gene            19404..20324
                     /gene="pyrB"
                     /locus_tag="PC471_000803"
     CDS             19404..20324
                     /gene="pyrB"
                     /locus_tag="PC471_000803"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HDF2787943.1"
                     /translation="MLKSRNLIQPEDFSIEEIDEILELAQKIIDNPSKYSRICEGKLL
                     ATLFYEPSTRTRLSFESAMNRLGGRVVGFSEPNSSSASKGETLGDTMRIVSGYVDIIA
                     MRHPQSGAASEAARYTEVPFINAGDGKNQHPTQTLTDLLTIKSLKGTLESHTIGLCGD
                     LKYGRTVHSLVKAMARYKNTKFVFIAPEELKMPDYIKEAIKGHAYYETNNLDDVIGSL
                     DVLYMTRVQQERFEDKSEYERLKNYYILNKAKLEKASKDMLVMHPLPRVNEIDIDVDS
                     DDRAVYFKQAKYGMYVRMALIIKLLGINED"
     gene            20363..21055
                     /locus_tag="PC471_000804"
     CDS             20363..21055
                     /locus_tag="PC471_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HDF2787944.1"
                     /translation="MYKILENIYIGEDMYRMKVKGNFEGKMGQFYMLRAWDTYPVLSR
                     PISIHDIDEEGITFLYKVVGEGTQILSNLKVNDNIKLEGPYGNGYAKVDGKVALVGGG
                     IGVAPLYLVAKNIKNCDAYLGFREDVILEDEYKQVCNKVYTTVGNTFVTDIIDVEKYD
                     YILTCGPTPMMEKLVKMVEGTKTRIMVSLENHMACGVGACLVCTCKTNGGNKKTCKDG
                     PVFWGEDVIFNG"
     gene            21048..21950
                     /locus_tag="PC471_000805"
     CDS             21048..21950
                     /locus_tag="PC471_000805"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HDF2787945.1"
                     /translation="MGNLSVKFGSVEFKNPVIMASGTFGFGKEYNEIYDIQKLGGISS
                     KGLTLNKKPGNNGMRVHETSSGMMNSVGLENPGVQGFIDYELPFFSKLDLVRIANVGG
                     GTLEDYLLGVQMLNDKPIDIIELNISCPNVKAGGMAFGIKNEVAREVVREVRNITKLP
                     LVIKLSPNAEDIVGMAKVCEEEGADGVSLVNTFKAMAIDIKNRRPVFENVYAGLSGPA
                     IKPIALRMVHEVCKNVNIPVMGMGGITKATDAIEFIMAGATCIQVGTANFMNPRIGIE
                     IIDGINEFMDREGIKSLDEIRGII"
     gene            22035..22619
                     /locus_tag="PC471_000806"
     CDS             22035..22619
                     /locus_tag="PC471_000806"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HDF2787946.1"
                     /translation="MSKVDVVDILKKSDALLEGHFLLSSGKHSNRYVQCAKVLRFPEY
                     AAEVLSTVVEQIKDLDIDLVVGPAMGGVIVSYELGRQLGKEAVFTERKDNTMELRRGF
                     EVKKGAKIIIAEDVVTTGKSTMETKRVLEALGGEVVGVACIADRTNHDIGMPIYSAIK
                     LDIQVYESDECPLCKEGKLPVVKPGSREFKELGM"
     gene            22833..23759
                     /locus_tag="PC471_000807"
     CDS             22833..23759
                     /locus_tag="PC471_000807"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HDF2787947.1"
                     /translation="MKNQLNENHKKVVFFGVGAVGATFAEQFFNSKYDFKILCDNERK
                     KRYLEEGFIINGKRYDFDYVTKDEYKQEADFIIIGLKYNNLKENIKELDGLVGKNTVI
                     MSLLNGVDSEEIIGERFGIEKMVYSYVTNIDAKKINNNIIHTTNGIIVFGNKDNSEDR
                     KTNIITEVFDDVNIEYTLSKDIQRDMWWKYMVNIGVNQTSAILGAPYGVFQSSEHLRE
                     LAKSAMREVVAIAQAKDISLTEDDVEHSLHRILEHSKEGRTSMLQDVEAHRLTEVDMF
                     SKNICKLGKKYNIPTPINQTFFYMIKVIESRF"
     gene            complement(23811..24701)
                     /locus_tag="PC471_000808"
     CDS             complement(23811..24701)
                     /locus_tag="PC471_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDF2787948.1"
                     /translation="MFEELKTFVAVVEYKNFTKAGEYLNLSQPSVSKHIKNLENYFKV
                     VLINRSIKQKTIFITESGQILYKRAKEILNLLNITYHDVSQVSDAITGHLKIGASLTI
                     GEYILPNFLALFSKKYPDIDVEVFIKNTSIVSSHVKDYILDIGLIEGTCSSPSFIQEY
                     FFEDKMVLALPYKSHLLKDFSFDKLQNQKWIVREDGSGTRDYLDMFLSVKEIIPKSMM
                     VFGSNYAVKESVRNNLGITIVSNLVTSLPVLNNELSVIELGSSYNRHFSYIFPKDITL
                     SKAATIFIEELKIFSNLNSI"
     gene            24889..25920
                     /locus_tag="PC471_000809"
     CDS             24889..25920
                     /locus_tag="PC471_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HDF2787949.1"
                     /translation="MLKKKSSLTLKTIREILPGLFVSVLVGYISIFISILIPKVGAAS
                     ISIFLGMFVGNLFLNQKVFQKGYKFSETDLLSYSIVLLGATLSVSTLIDLKVSGILFI
                     ILQMTITIIAALYIGKKLGFEENFRFLMASGNAVCGSSAIGATVPVVNATDKEKGIAV
                     TIVNVTGIFLMFLLPIISQFLYSHELVKTSAMIGGTLQSIGQVVASGAIVGENVKDLA
                     TIFKIVRVIFLVVVVLVFGHMKNQSNREILEEEKDEIKKKKVTIPWYVIGFFITCALF
                     SMNIIPKEVSVLCKEISNKLEIIALAAIGLKVNVKDLVKQGKEVSLYGLFVGIVQVVS
                     AVVLIKIFI"
     gene            26214..27089
                     /locus_tag="PC471_000810"
     CDS             26214..27089
                     /locus_tag="PC471_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA glycosylase"
                     /protein_id="HDF2787950.1"
                     /translation="MNVYEKGNGVILEGVTDFDPVHIFECGQCFRWHKQEDGSYTGVA
                     KGRILNVKKENDKIYLNNTNLKEFNSIWYNYFDLGTDYTEIKNKLKNMDEYLNKATEF
                     GWGIRILRQDGWEMLISFIISSNNRIPMIQRAIENLSRKFGKYIGEYEGNEYYAFPTP
                     EELNKASQEEIRACQTGFRDKYIKSTTQAVIENNDEVSEYTNLSTEDCRKELLKFNGV
                     GPKVCDCIALFGMQKYDSFPVDVWVKRVMQEFYIDEDMSLPKMRTYGIDKFKEMSGFA
                     QQYLFYYARELGIGK"
     gene            27107..28597
                     /gene="cls"
                     /locus_tag="PC471_000811"
     CDS             27107..28597
                     /gene="cls"
                     /locus_tag="PC471_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HDF2787951.1"
                     /translation="MGVIGTIFLFYLIISYLAGAIISVIILLENRDPAKTMSWLLMFI
                     IFPGVGLMIYAISGRNIRKRKLFKTQKLANNIKEKKLFDTLEKITEIVELEKESIKQN
                     KLLRDEEDGSYRKRVINMLLKTGMFPFTKNNKVDVFVDGNEKFKRLIEDIREAKDHIH
                     LEYFIIKDSEIGRVLKEELIKKAKEGIKIRILYDDVGCWRFWFNRKFFREMREVGIEI
                     AAFLPTKFPIIGGKLNYRNHRKIVVIDGIIGYTGGINIGDEYLGKNDKFGYWRDTHIR
                     IKGISVYMLQMTFLIDWYYTTKEVLVTKNYFPSVGNVGESMIQVVASGPDSDWEDIHY
                     AYFSAICQARKNVYIETPYFIPDESLLKAIKSAALSGVDVRIIFPKIADHKIVNIASY
                     SYFEEILRAGGKVYLYNKGFIHSKVVIIDDKIASAGTANMDLRSFMLNFEVNAFIYDE
                     EVIRVMTDDFFEDLSHCEELNLEVFKNRNIIQKIKESVARLFSPIL"
     gene            28790..29074
                     /locus_tag="PC471_000812"
     CDS             28790..29074
                     /locus_tag="PC471_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012062101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HDF2787952.1"
                     /translation="MKIRPLADRVVIKKVEAEEKTASGIVLPGAAKEQPQIAEVVEVG
                     PGGIVEGKEIKMELTVGDKVIFQKYSGTEVKIEGQEYTILRQSDVLAVIE"
     gene            29107..30735
                     /gene="groL"
                     /locus_tag="PC471_000813"
     CDS             29107..30735
                     /gene="groL"
                     /locus_tag="PC471_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729227.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HDF2787953.1"
                     /translation="MAKEIKFSEETRRALEAGVNKLADTVKVTLGPKGRNVILDKKFG
                     SPLITNDGVTIAKEIELEDRFENMGAQLVKEVATKTNDVAGDGTTTATVLAQAIIREG
                     LKNVTAGANPILLRKGIQKAVTVAVEELKNQSRIVETQEAISQVASISAGDEEVGKLI
                     AEAMEIVGKDGVITVEESQTMNTELDAVEGMQFDRGFVSAYMVTDVDKMEAVLNDPYI
                     LITDKKISNIQELLPVLEQIVQQGKKLLIIAEDVEGEALSTLVVNKLRGTFDVVAVKA
                     PGFGDRRKEMLQDIAILTGAQVISEELGYDLKEADLSMLGRASSVKVTKESTTIVDGS
                     GDKKAIEDRVTQIKHQVEQTTSDFDREKLMERLAKLAGGVAVVKVGAATEVELKERKL
                     RIEDALNATRAAVEEGIVAGGGTAFVSVIPAIGTLIESLEGEVKLGAQIVKKALEEPL
                     RQIAINAGLEGAVIVQNVVNSEAETGFDALNEKYVNMIEAGIVDPTKVSRSALQNAAS
                     IASTFLTTESAVADLPEKEDAGMPGMGGGMPGMM"
     gene            30997..31737
                     /gene="larB"
                     /locus_tag="PC471_000814"
     CDS             30997..31737
                     /gene="larB"
                     /locus_tag="PC471_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarB"
                     /protein_id="HDF2787954.1"
                     /translation="MDLRKLLEEVKNENIDIDFALEKLKDLPYEDLGYANIDHHRELR
                     NGYPEVIYCEGKSDEHILGIIDKMNQKSSNILGTRCRKETFLKIKEIYNHAEYEEASK
                     ILKIQNHDIENIGKGKIVIATGGTSDIPVADEAYHTAKFLGNDVDRIYDVGVAGIHRL
                     LNKRHKIDSARVIVAVAGMEGALASVIGGLVDVPVIAVPTSVGYGANFGGLAALLAML
                     NSCASGISVVNIDNGFGAGYLASMINKL"
     gene            31821..32555
                     /gene="larC"
                     /locus_tag="PC471_000815"
     CDS             31821..32555
                     /gene="larC"
                     /locus_tag="PC471_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarC"
                     /protein_id="HDF2787955.1"
                     /translation="MSEKILYFDIISGISGDMTLASLLNLGVPKEIFLEEIHKLRMSD
                     EFNINISSKTENGIVGTKVEVITKEKHTHRNLVDIFEVIDKSTLNENVKSKAKKIFMV
                     IGEAEAKVHGTTIDKIHFHEVGAIDSIVDIVGASILVDLLCVDKVCASTVSVGSGFVK
                     CEHGIIPIPAPATIEILKGVPIKLNNVNGECTTPTGAAIIKVLCDEFVDELEFSPKQI
                     GYGIGHKKFEVPNILRTVLGEKKKKN"
     gene            32585..33004
                     /locus_tag="PC471_000816"
     CDS             32585..33004
                     /locus_tag="PC471_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF111 family protein"
                     /protein_id="HDF2787956.1"
                     /translation="MSSEIYSYLYEKILDEKALDIYTESIYMKKNRPATKVSILCSES
                     DLEKFIKILLLETSTFGVRYNAYNREILERKFVKLDTKYGVVTVKLGYYKGKLVKATP
                     EYNECKTIAKNEDIPIKKVFADINFRIKEEFSENLLT"
     regulatory      33064..33165
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            33386..34921
                     /gene="guaA"
                     /locus_tag="PC471_000817"
     CDS             33386..34921
                     /gene="guaA"
                     /locus_tag="PC471_000817"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000743900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HDF2787957.1"
                     /translation="MKHELVLVIDFGGQYNQLIARRVRENNVYCEILPCTASIERIKE
                     KNPKGIIFTGGPNSAYLEDSPTISKEIFELGVPILGICYGIQIMSHVLGGVVRKGNKQ
                     EKEYGKTAITYGKSSLFEGITTNSVWMSHTDLIEKVPEGFTIVANTNDCPVAAMENVE
                     RNLYGVQFHPEVEHCLEGDKILTNFLYNICKVKGDWTTDSFIEDKIKELKEKIGDKKA
                     LCALSGGVDSSVAAVLIHKAIGDNLTCIFVDHGLLRKNEGNDVERIFREKFDINLIRV
                     NAEDRFLSKLKGVSEPEAKRKIIGEEFIRVFEEESNKLGKMDFLVQGTIYPDVIESGH
                     GNAATIKSHHNVGGIPEDVDFQEIVEPLRELFKDEVRKIGLELGIEEGLIFRHPFPGP
                     GLGIRVIGDVTKEKCDILREADAVYMDELRKAGLYREIWQAFATLPDVKTVGVMGDER
                     TYAYLVGLRAVTSSDGMTSDWYKMPYDVLERISNRIINEVDGVNRVVYDITSKPPGTI
                     EWE"
     gene            complement(34998..36044)
                     /locus_tag="PC471_000818"
     CDS             complement(34998..36044)
                     /locus_tag="PC471_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003460085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDF2787958.1"
                     /translation="MPAYKDEQRKSWYASFYFTDFDGDRKKKIKRGFKTKKEALEFER
                     EFLNKSKMSTDMSFESLIEEYMHDMSSRLKLSTLETKKYLINFRILPFFKSLKINEIT
                     ATHIRRWQNELLKSDYSQTYIKTINNQLVAVLNYAVKYYNLPSNPAHLAGSIGKKNAD
                     EMNFWTLEEFKKFIEFEKKSEPRLAFEILFWTGLRLGELLALTPKDIFENKISVEKSY
                     IRLNGEDIVSSPKTPKSKRVVPIPHFLYNNIKDYLSKLYDLKDNERIFKFAKSYLSKE
                     LDRCCKLSNVKRIRVHDLRHSHASLLVNMDVNILTIAERLGHEKVETTWNTYSHLYPN
                     KQLEVAQKLDNLNI"
     gene            complement(36121..36336)
                     /locus_tag="PC471_000819"
     CDS             complement(36121..36336)
                     /locus_tag="PC471_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF2787959.1"
                     /translation="MLKKLRKKKRMTQLELAEKMRRNRSYISKLENQEYKDIGISTIL
                     DLSIALEEDFLELCKYYKLQEIKRRGK"
     gene            37021..37158
                     /locus_tag="PC471_000820"
     CDS             37021..37158
                     /locus_tag="PC471_000820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2787960.1"
                     /translation="MGNSDCSKMLAFYEIMKMLETIDEAKFYNCKEEIKSEFKHIKKE
                     D"
     gene            complement(37186..37578)
                     /locus_tag="PC471_000821"
     CDS             complement(37186..37578)
                     /locus_tag="PC471_000821"
                     /inference="COORDINATES: protein motif:HMM:NF024252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2787961.1"
                     /translation="MYKDESNIFSKRLREEREELGLMQKEMANKLSLPANTYNGYETG
                     KRSPALDVVRHIADTLDISTDYLLGRTNIKINISNITEKELIKKLNPSDDMKEILDIF
                     SELDDDSKNKALKIAKLFLEEQNTKNKE"
     gene            37766..37960
                     /locus_tag="PC471_000822"
     CDS             37766..37960
                     /locus_tag="PC471_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008422227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2787962.1"
                     /translation="MKNYILKSLRVRQGLRQRDLAKILGMNPSTYSSKENGDRRFTVS
                     EAIKISDFFNTDLRDIFLNK"
     gene            37996..38790
                     /locus_tag="PC471_000823"
     CDS             37996..38790
                     /locus_tag="PC471_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HDF2787963.1"
                     /translation="MNNLQVIERNNERVLTTQQLADVYETDVNNIQTNFNRNKERFKE
                     NVHYFLLQGEYLKEFKNQPTNSQLVSKHSSQLYLWTERGANRHCKILDTDKAWEQFDN
                     LEETYFKVKQHKPTCIEDVLIESLKEMKDLRLQVNQANSIALEAKTEVETIKDVVSLD
                     SNSWRTNTHQLIARIAKKQGGFEHINMLRTESYELLNKRFGVDLHRRLINKRRKMAEE
                     GVSESKREKVNNLDVIQDDKKLIEGYVAIVKDMALKYGISSDLSKN"
     gene            complement(39089..39307)
                     /locus_tag="PC471_000824"
     CDS             complement(39089..39307)
                     /locus_tag="PC471_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016056353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2787964.1"
                     /translation="MTKFKDNIKLVRKQMNMTQKQFASLFGISERAYQYYESGSREPN
                     IETLILISNKLNVSTDFLLGLSPNQNRN"
     gene            39426..39614
                     /locus_tag="PC471_000825"
     CDS             39426..39614
                     /locus_tag="PC471_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014017802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2787965.1"
                     /translation="MNNNLKLQREKVGLTQLEVAQKAKITERSYQYYEAGERLPNIRT
                     ALKIARILNTNCEKLFNE"
     gene            39656..40450
                     /locus_tag="PC471_000826"
     CDS             39656..40450
                     /locus_tag="PC471_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015994369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor KilAC domain-containing
                     protein"
                     /protein_id="HDF2787966.1"
                     /translation="MNNLQLNNKNTITTLEIADMLEIRHWEVLRKLEGTEKTKGIIDI
                     LNDNNFVVVDYFIKSTYLDSKNESRPCYNVTKLGCDFLANKFTGEKGIIFTARYVRRF
                     NEMERVLKEQQPKLPTTYKEALQQLLIEVEEKEQLQLENQEKDKVIQLQQPKVLFADS
                     VASSNDSILIGELAKLLKQNGIDTGEKRLFAWLRDNGYLIKRKGEDYNTPTQKSVDLG
                     VIETKEGARVHPNGYISVTKTPKITGKGQVYFINKFKSSKQLSMLS"
     gene            40532..40744
                     /locus_tag="PC471_000827"
     CDS             40532..40744
                     /locus_tag="PC471_000827"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2787967.1"
                     /translation="MKNNTSDSRVKYFCKCPYCGFDNEVEVKKGLKPKICCICTKEVE
                     YEKLEQQSALENTEYFESRCIVWQKQ"
     gene            40729..40935
                     /locus_tag="PC471_000828"
     CDS             40729..40935
                     /locus_tag="PC471_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015558246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2787968.1"
                     /translation="MAKAVAKEQLFYRAKDIAKILDVCEATAYKIIGELNKELEKEGW
                     KTFPGRVSVTYFKERYCYKTKKGA"
     gene            41107..41334
                     /locus_tag="PC471_000829"
     CDS             41107..41334
                     /locus_tag="PC471_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2787969.1"
                     /translation="MKVLEVNFPTEVLKVGKAIRVTCERISFESDCIIIHSHEFELTV
                     VYFDKELDDLVQSSITIEDAIGHDCCIEILN"
     gene            41347..41664
                     /locus_tag="PC471_000830"
     CDS             41347..41664
                     /locus_tag="PC471_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2787970.1"
                     /translation="MKSLIIVRNAVEQQLNRANLEINKNEELYTKLRKKEKRDISDEI
                     ELSNALREKSVNERLKIFAESLLKIIDTQIEIKEYEESEDYKIFQLISEELERDKPID
                     VQI"
     gene            41749..42264
                     /locus_tag="PC471_000831"
     CDS             41749..42264
                     /locus_tag="PC471_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siphovirus Gp157 family protein"
                     /protein_id="HDF2787971.1"
                     /translation="MSTLYELTTDLLEIEEGLTETTGNEAEKLEEIKEIIKKEIQNKN
                     TRIISVIINIDSDINSIDSEIKRLQELKRVKKNTLDRLKNNIKDCMELLGTKKVETVL
                     GNISIRKSAGSLVIEDEEKIPGIYKTVEQVIKVDKNSIKDFIKKGHEVEGCRIEYSTT
                     LIIPKAKKEVN"
     gene            42266..43168
                     /gene="bet"
                     /locus_tag="PC471_000832"
     CDS             42266..43168
                     /gene="bet"
                     /locus_tag="PC471_000832"
                     /inference="COORDINATES: protein motif:HMM:NF015776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage recombination protein Bet"
                     /protein_id="HDF2787972.1"
                     /translation="MNNKALELATCTLESGQILDYTTVKNYLVSGNGNVTDQEVLMFI
                     ELCKAQKLNPFIKEAYLIKFGNSPANIVVGKDVFVKRANKNPNFEGMKAGIVTVNKNG
                     EVFEREGSLKLPQEELIGGWCEVSVRGMKFPIKSVVSLEEYSKSQATWKQMPCVMIRK
                     CAIVTALREAFPEDLQGLYDSAEIKTVPDKLPQKPIEIGKASPSQKQGILKLASMKGL
                     YDYENKKDTSKLEEFCKSNGFDLKELKFEEVDELIELLSEYEPKDDFIDAEFEEVKED
                     EVEVREEAENIDCQISMEDNMNFE"
     gene            43181..44014
                     /locus_tag="PC471_000833"
     CDS             43181..44014
                     /locus_tag="PC471_000833"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2787973.1"
                     /translation="MPIFRQIYTNFWTDPKIQEEFSVEDKLFYIYLLTNPHTTQIGIY
                     TITKKQIAFEIGWTLESTNAVMDRFINHHQLINYNSETREIAIKNWAKYNLNRAGTPM
                     ENCVRKELREIKDKSLLMLIYEHIENKKFKQIFEEYFDELRNGVRNETRDEGNNNNNN
                     NNNNNNNNNKEVAVVVDKIKKYFDLESKDLEKIVDVFIHTNKGIDYLEEKLRLVKNTE
                     SVKSVTGYLIKALQEDYKPIPSKHNKNKFHNFNQTFDQYTDKELCDMANRGQLEESKF
                     G"
     gene            44085..44339
                     /locus_tag="PC471_000834"
     CDS             44085..44339
                     /locus_tag="PC471_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2787974.1"
                     /translation="MNELVKITDKEIAKCIKLQKGFAKKKDYPHFAPKDGICCRCGRN
                     IYQNYEIRFFKEARISKGYANIAGKELITGCPHCSRTFCD"
     gene            44414..44812
                     /locus_tag="PC471_000835"
     CDS             44414..44812
                     /locus_tag="PC471_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2787975.1"
                     /translation="MELKFREWNKNGKEMYSYDEMMCYSKNLLREWVYSGVYLPTSNE
                     NFEVMIYTGLKDCIRKEIYEGDIVSYILSFEEFIGEVKFEEGFFVIDNEMLGENVGLF
                     HEIAVVKVIGNIYENPEMLEKIRKPKVLED"
     gene            44815..45066
                     /locus_tag="PC471_000836"
     CDS             44815..45066
                     /locus_tag="PC471_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2787976.1"
                     /translation="MIIIRSQDRLDLMRVNRVEIYDKQVFVIFEDDVKRIGVYESNER
                     AMQVLNDIQKFIENGVRTDYIDSCRVRHNQEKVFEMPVE"
     gene            45082..45438
                     /locus_tag="PC471_000837"
     CDS             45082..45438
                     /locus_tag="PC471_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016056367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2787977.1"
                     /translation="MAKVWVDAGTFLERTKDIEDMFEFNLRKVRDRNKKANVIDLRVN
                     DEGQGRKGKKVECFNIVIGETKVFNTAVEASKYVYFTDVYIAHLARTGKTTKNGWKVR
                     YIQEVADGISKCGTSN"
     gene            45407..45556
                     /locus_tag="PC471_000838"
     CDS             45407..45556
                     /locus_tag="PC471_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2787978.1"
                     /translation="MVLANVEQVIKLAEKILNKKKCSVNKAIDIAIKILSKYECEGMI
                     RNEFN"
     gene            45543..46001
                     /locus_tag="PC471_000839"
     CDS             45543..46001
                     /locus_tag="PC471_000839"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2787979.1"
                     /translation="MSLIKYRGYDFENEKWIYSETIKWSDAVDCLFMLKENCEWQKVS
                     NVGVFSKCWSGNNEEIYEGDILKEPYNTKNKYEYGIVRQENYALELYVEWHYLKQFEG
                     KWEEITSKATIMNSKKYVVVGNECENLEEVRKEFLESKEKLENENTNAVN"
     gene            45976..46134
                     /locus_tag="PC471_000840"
     CDS             45976..46134
                     /locus_tag="PC471_000840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2787980.1"
                     /translation="MKIQMQLTKDKESFKVCVNSEEEELEKLFDELVSQMLACRRKNK
                     KVQGDIEK"
     gene            46131..46304
                     /locus_tag="PC471_000841"
     CDS             46131..46304
                     /locus_tag="PC471_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3797 domain-containing protein"
                     /protein_id="HDF2787981.1"
                     /translation="MKLKDIIKLGEKYCYCPHCGNDKIGSNEGKLIVEEHTYYRECSC
                     GFSILIDDRKDEI"
     gene            46422..46580
                     /locus_tag="PC471_000842"
     CDS             46422..46580
                     /locus_tag="PC471_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2787982.1"
                     /translation="MNDRLEMINASVNYIQMICESSNIAIIAEHDRVRILDLETKEKY
                     DLLRSEGD"
     gene            46584..46907
                     /locus_tag="PC471_000843"
     CDS             46584..46907
                     /locus_tag="PC471_000843"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2787983.1"
                     /translation="MSKVIQCDFCKEIFEKYNGECIELYKKDCSKGVLGIDKHICPTC
                     YEKFIEGKIEKVENAKRFKDKLIDFLVEHEVCDCCACDCVFDSNDEKKCKAGIREWVE
                     SEVEE"
     gene            46909..47208
                     /locus_tag="PC471_000844"
     CDS             46909..47208
                     /locus_tag="PC471_000844"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2787984.1"
                     /translation="MAKLIVSIPLTKYLKDITKEWGETYITKTYGGHVYLSDEAPGEV
                     FKKEDDLNYIEFNDSDIVMRLPKEVHQHINLKKGCNKNLKDVLKEIKKIEEKTNI"
     gene            47315..48388
                     /locus_tag="PC471_000845"
     CDS             47315..48388
                     /locus_tag="PC471_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HDF2787985.1"
                     /translation="MLTFLDLFAGIGGFRLGMEKAGHKCLGHCEYDKFANLSYNAMHK
                     PKEDEWFERDIREIRTENIPRADVWCFGFPCQDISVAGKQFGFRGERSSLFFTVTKLI
                     RELKEEDRPKYLLIENVKNLLSVNGGFDFLKVLVELDEIGYDAEWQVLNSKNFGVPQN
                     RERIFIVGHFRGRSTRKVFPIERKSGKNLEQLNNPTHSTNRIYDAVGIARCIRSQAGG
                     GGAKTGLYFIDLNKNSKVTINARCLKAKYNAGVTNRNCDNSGVLVNAVLTPDRVNKRQ
                     NGRRIKESGEPMFTLTAQDKHGILKNGDIRRLTPKECFRLQGFPDKYYERAASVCSDS
                     QLYKQAGNAVTANVVYEIAKRMG"
     gene            48472..49167
                     /locus_tag="PC471_000846"
     CDS             48472..49167
                     /locus_tag="PC471_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2787986.1"
                     /translation="MDLNKIMNDALVEIEESGFVEETVKKELEETIKRAVNEIFGSYS
                     KFRKNIEQHLGENININLDELDIQKYNLLVANVVKEKVDATMKGQGIEHLKRNLDNML
                     VGLKKEYKMSELLEELKEDKYNLDEYPCDEDCITFILESNFNSEWARWIDFDECPNVS
                     RNNCEHSILLRNDGTIAAFRYQNREISSKDIMNGFGNFGDLLFKIYAHNSKIILDLGN
                     DVDDYDLTNGEDY"
     gene            49185..49406
                     /locus_tag="PC471_000847"
     CDS             49185..49406
                     /locus_tag="PC471_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2787987.1"
                     /translation="MSKYVLRWQKGLLLDERKINYSCGSKEMLKQKAELLAKDDKILL
                     VTIDKVEEVIKDTRSQKMAEYYCDGGIEI"
     gene            49403..>49608
                     /locus_tag="PC471_000848"
     CDS             49403..>49608
                     /locus_tag="PC471_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HDF2787988.1"
                     /translation="MIIHKFIIHVLDKNSDTPILNDFEGRVSQDIEAFFQKKISKVSR
                     DNDIRTAVFNDYSNNLIKKCCEQI"
BASE COUNT        19747 a         5308 c         8769 g        15784 t
ORIGIN      
        1 tttgtcaagt ttaatttagt tgtaatttta tcttttatta aaaataatat aaattaaaac
       61 atataaataa aatattttat acaatatatt gtaaaggagg taactttgat gaaagcagaa
      121 aaaacaagta tatatccaat tattaaaaat aatttaaaca aaatatcaat tgttgaatat
      181 aaaaagggtc aaagatttgt agcatcagat aaagacttac aagaagtgtt ctttataata
      241 gagggagttg cattagtgga gtgtactact agaggaggaa ataaatttct tgttgacatt
      301 gtacctgaaa atgaatttat aggcaaaatt agttacatat atgaacataa tttgaaatgt
      361 gatatatttg caaagaccaa tataaaatta tttcgatttg aaaaaaatgt atttgaagag
      421 ttttatctca aaccagattt tattgcacta tttcacagaa aatgtacaag aagaatatat
      481 gaattatata aaaccagaat ggtaagagaa ctattttctt gcacagaagt tattgcctat
      541 tgtatatcaa ataaacaaga aaataatgtc tgcaatataa aagctattcg taatttcagt
      601 gaaatctatt caattagtag aaaaagtaga taccattctc tgctaaagtt aactgaaaag
      661 gagatagtaa aaaaaatggg taatacatat gaaattttaa actatgatga attacataaa
      721 ttggcatttg aagtaaaaga atttttggaa gatgagtaaa aattgttact taatcaaaat
      781 aaagtataat aatttctgta aaaaatagaa atagtctcac tgaaaatcaa aaatacttta
      841 gttattttca atgagactgt ttagaaatat tgtatactta taatgtatag tataattatt
      901 aatcaatttc ctttactata ttaatagccc attttttaga taatgctcta ggaacaccaa
      961 atataaactt aaccacttct tcactataag tgagcaaaac gagtacaaat ataggtgcac
     1021 ctttaaatgc agctataaaa gttagaggaa tagaagctaa ccacatacat cctaattcta
     1081 aaaatagagc atatttagta tctcctccac ttcttaaaat accaattata accaatgtat
     1141 taaaagattt aagagccatt agtattccca ttataaagaa tatcttagtt atatcagaag
     1201 ctaagctatc agaaacacta aacattttta ataataatgg tatatttaaa attaaaataa
     1261 ttccaagtat taaaccagct gaaaatacaa gtatagaaaa ctttttagca tattcaatgg
     1321 ctcttttaat atggtcagct ccaagttcat ttccaagcat aatagatgca ccagatgcaa
     1381 tacatacagc tgtcattatg aagaaattac ctgtactggt tgctatctgg ctagctgcta
     1441 tagcagaagt accagctctt gcatatgcaa cagaatacag gacagttcca attgcccata
     1501 cagagtcatt tacaaatata ggaaagcttt ttgatgaaaa agattttata aaaattttat
     1561 caatagcttt tagatttttt agaccaaact ttaatatata gtctttttta taaaggtaaa
     1621 cataagtaat cattaaaatt aattcacata ttctagcaat aacagtagct agagcagcac
     1681 cttttacacc caatgcagga agaccgaagt taccaaatat aaatccataa tttaaaataa
     1741 catttgttac aagtgcaaat gcactacaaa tcatacctaa cttaggattt ctaacacctc
     1801 ttgaaccagt gctaaataca gtacttacag caattaatgg ataactaaat actgcaatac
     1861 taaagtaatc tatacaaagt tttaccacct cagaatcata cgaaaatatg tgtataattg
     1921 gctttggtgt tagtaaagca ggaataacaa atacaaaact taaaacaatt gccaatacac
     1981 aagtaagacc tgtgactttt cttatattac tcacatcctt ctttccataa aactgtgaaa
     2041 taaaaactcc agcacctcct gtgataccag ataaggacat attaaaaaga aagaaaaatt
     2101 ggtttgcaac tccaactgaa gctacagatg tttcccccaa actacttatc ataattgtat
     2161 ctattacatt tacagaagta gaaattaagt tctgtatgat aataggtata cataaagata
     2221 gaagtatttt ataaaatctt ttattttcgt cttttaaaga aaatagttct tgcaaataat
     2281 cctctccttt ttacataaat tcttcccact catcatatag agaggcaagg ttattttcta
     2341 aattaatttt ttcttggcta acttctttag ctttatctgg attagagtat acttcttctt
     2401 gacataaaag tatatctaat ttttcaattt caatctctaa cttttcaatt tctttttcta
     2461 tattttgtct ttttattcta tttttctttt ctatttctct ttgttctctc tcttttcttt
     2521 tttcatcttt tatttgagtt ctagtttttt cttctttttc ttctataata ctcatctctt
     2581 ggatttgcct ttttttatct atataataat cataatttcc cagatattca gtaattccat
     2641 tttcatctaa aactaaaatt ttatcaacaa cagtatttag aaagtatcta tcatgtgata
     2701 ttgtaaatat agtcccatca taattagtta atgcctcttc aaggacttcc ttagaatcaa
     2761 tatctaggtg attagtaggc tcatctaata acaataagtt tgcatttgat agtataagtt
     2821 taagtatggc aactctagct ctttctccac cactcaatgt agaaatcttc ttaaaaactt
     2881 cttcatctac aaataaaaat gcccctaaca tatttctcaa agtagtttga gtaagatgag
     2941 tattattttc ccaaatctca tcaattattg tgttatctaa attaagagtt ttttgttcct
     3001 gatggaagta ggaaacatgt acattagttc caaatttaat attgccacta agaggttgta
     3061 gttcattagt tattatttta aataatgtag atttgccaac accatttgca cctataagtg
     3121 caactttttc ccctctatat atatctaaat ccaagtcttt aaaaagtatt ctttctccat
     3181 aacccataga tacatctcta atttgaagaa catcattacc actctggaca gaaggcgtaa
     3241 attgtatacg agcttttttc ctataagctt ctggtttatc taaaacgtct accttgtcta
     3301 atgctttttc tttacttctt gcacgcttta ggtgtttttc tctaccataa gcttttagtc
     3361 tttcaattga ttcttcttgc ttttttattt ctttttgttg gtcttcaaat ttcttaagct
     3421 ctagttcttt ttctatttta gataactcaa caaatttaga gtagttacca ttatatactt
     3481 taagtttttt gttgtgaact tcaaaaattc tatttacgga ttggtctaag aaatatctat
     3541 catgagatat taacatgaca gtccctttat attgttttaa aaatacttct aaccattcta
     3601 ttgcttcaga gtctaagtgg ttagtaggtt catcaagtaa caatagagtt ggtttcttaa
     3661 gaagtaattt tcctaaaaga actctagttt tttcaccacc agaaagtatg cttattggct
     3721 tatccatatc attgtcacta aatcctaatc cctttagaac tcctttggct tcagatttat
     3781 atccatatcc atttaaatct gaaaataatt ctagtttatg tgaatattca tccatgattt
     3841 tttctataat tggagagttt gtttttgtac tttcttcaga tattttaacc tccaattctc
     3901 ttaaatagga ttccatttct atcaaatttt taaaaacttc tagtacttct tccaaaatag
     3961 tattatccga ataaaaattg gtattttgtt ctaaatatcc gatttcacaa tctttagaag
     4021 tgtaaatatc tccactatca tatccataaa ttcctgaaat tattttaaat aaagtagttt
     4081 ttccagtacc attaacaccg attataccaa ttttatcgcc ttcatttact gtaaaactaa
     4141 tattttctaa tatagaatct ataccaaaac ttttatttaa gttattacat gacaaaacaa
     4201 tcataaattt ctacctcctt gaactatcta aaattataat gttttgatat aatataatca
     4261 aggttatatg tattagtttt gaaaaagaaa tattttattg tgaatcaaac aaaaaagagg
     4321 acacttttag gaggtgaaga tgttgggaaa taaaaatata tcaatggcag ttataagaag
     4381 gctcccaaaa tatcatagat atcttggaga cttattagat agggatatac aaagaatatc
     4441 ttctaaagaa ttgagtgata taatagggtt taccgcttct caaataagac aagatttaaa
     4501 caactttggt ggatttggac aacaaggata tggttataat gtagaagctc ttcatactga
     4561 gataggtaaa attcttgggt tggatcgacc atacaacgca gttcttgtag gagcaggtaa
     4621 cttaggacaa gctatagcca attatgcagg atttagaaaa gctggattcg agataaaagc
     4681 tttatttgat gcaaatccta gaatgatagg tttaaagata agagagtttg aagtattaga
     4741 ttcagatact ttagaagact ttataaaaaa caataatata gatattgctg tattatgtat
     4801 acctaaaaat ggagcacaag aagttattaa tagagttgta aaagctggaa tcaaaggtgt
     4861 atggaatttt gcacctttag atttagaagt tccgaaaggt gttatagttg aaaatgtaaa
     4921 cttaacagaa agtttattta ccttatcgta tttaatgaaa gaaggaaagt agacaactta
     4981 aacatattaa taatgagcag ttgtgaagag tattgagaaa gtcagaagat ttaatattaa
     5041 atcctctggc tttttgaaat attaggataa aaatttaaat aatgtatgga aataaacaat
     5101 attaaggaga gtggtttttg tggaggaatt atggaaaaag gcaattgaat ttcatggtca
     5161 tgaatgtcca gggttagcta taggatttaa agcagcactt gcagcaagag aatatttagg
     5221 atgtaaacag tcaggagatg aggaaattgt atgtatttct gaaaatgatg cttgtggaat
     5281 agatggtata caagtcgtgc taagttgcac aatgggtaaa ggaaatttac tacttaggat
     5341 gactgggaaa tcagtatatc atttctttaa tagggaaaat ggaaaatcta taagaatata
     5401 tgtaaagtca aagccatttc aaatggatag agaaggatac aaaaagtact tattagaaag
     5461 tgattttgaa gaaatatttg agattacaga tactaagtta agacttcctg aaaaagcaag
     5521 aatatttaaa tctcttaaat gtgaagtgtg tggtgaattt gcatctgagc aatatataag
     5581 gattgaagaa ggaaagaaag tatgtcttga ttgctttaat aaatatgaaa gattttatga
     5641 ggagtattaa aattataaat tagattaaag ttaaaagtat tagatatgtg ctaacagtat
     5701 tttaagatga attaggtagc aacataataa agtatataaa taatattaat tgaatcatac
     5761 ataattaaaa agaggctgat tagtatttat aaaataaaat cagtctcttt atgaataacg
     5821 aattattatt ctggttgagg tgcatctaca ggacaagctc ctgcacaaga accacagtct
     5881 atacaagaac cagcatctat aacatatgcg tcgtctccag ctgatataca gctaacaggg
     5941 cattcagcct cacaagcacc acagcttata caagcgtcag taattttata tgccattttg
     6001 aaatcctccc ttttatatgt attgtaattc aataacatta taacaaatat tattgaatat
     6061 aacaattaaa ttaattctac aacaaaaact ttattatgat attcttgttt taaagtttta
     6121 caataaagtt taatctgctt atatttttat ttattttaaa gaagtttatt ttaaaaataa
     6181 aattaaattt aaaaagttat gtattgatta tatttaaaaa tgttatactc taaaaaagta
     6241 tattttattt aaaaatttat taatacaaag gagatttact atgaaaaata aattagttta
     6301 catatgtatt atgagtctat tgctatttgt agtaggatgt tctaataatc caaataagga
     6361 aagtaccatt aaaaaagact ctaagaataa ccaaagccaa gttaaagaag aaaaaagaga
     6421 acctgtgaaa aaagatataa ctataagttt cgctggtgat gtaactatgg ggaactataa
     6481 aggttcatca tattatggca gttttgacca tgaatttaaa agacaaggag aagattacaa
     6541 ttattttctt aaaaatgtaa aacctgtatt tgaaaaagat gacttaacag tggttaattt
     6601 agaaggtcca cttactacag cgtcaaatgc aaaagaaaaa aaatttgcat ttaaaggaaa
     6661 gcctggatat gtaaatattt taaaatctgg aagtgtagag gcagtaagta ttgcaaataa
     6721 tcattcagaa gattattttg aagaaggaat gaaagataca cagtttatat tggacgagaa
     6781 taaaataaat tattttggat tagggaaaga tgctgttgta gatgtcaaag gaattaaagt
     6841 gggtctatta gggtataatg gattatcaac tgaatataat gaaaaaaatt taaaacaaat
     6901 ggaagatgat attaagtcct taaaaaagaa atcggatgtt gttgtagtgt actttcattg
     6961 ggggatagaa ttagattatt atccagataa aaaacaaaaa gattttgctc attatgctat
     7021 agacaaggga gcagatttag ttatggggtc tcacccacat gtgattcaag gaatagaaaa
     7081 gtataaaaat aagtatatag cttatagtct agggaatttt tgctttggag gaaataagaa
     7141 tccttctgat acagatagtt atatatatca acaaaccttt actttttcag ataataaatt
     7201 aacttcaatt aaagagccaa atataatacc aacttcaata acatctagta actccagaaa
     7261 taattatcaa ccaaaaatat tggatggaag tgagaaggat agagttttaa ataaacttga
     7321 aaaaataagc aaagatttaa ataaataaaa tgtaaaaaaa aataaatata tatatttatt
     7381 ttaataattt atattttgtt tagggtatgg taaatcgtgg tattaaataa gtagatttta
     7441 atagttaaat tatagtcaat tataataaag ctatttaatc tactttttta atataaaaat
     7501 ataaggggga atttgtaatg gcttgtaaca cttgtaaaat gtgcgaaagt gcagacaaga
     7561 aattagaaag ttttgtagct tcaaaggatg ttgaaacagc atttcataga acagaagatc
     7621 agaaagttaa gtgtggtttt ggacttcagg gggtttgttg tagactctgt tcaaatggac
     7681 catgtagagt aactcctaaa tcaccaagag gtatatgtgg agcagatgca gacactatag
     7741 tagcaagaaa ctttttaaga gcagtagcat caggagcagc ttgttattta catgtggtag
     7801 aaaatacagc taagaattta aaaaatgttg gaataactaa aggtgttgtt aaaggagaaa
     7861 aaacattaaa tcaattagca gagatgtttg gaatagaaag tgatgaaaag tatgataaat
     7921 gtataaaggt tgcagacaaa gttatcaacg atttgtataa atcaagagat gataaaatgg
     7981 aattagtaga aaaaatagct tatgcaccta gagtgaaaaa gtggaaagag ttaggtataa
     8041 tgcctggtgg tgcaaagtca gaagtttttg atgcaatagt aaaatcatca acaaatttaa
     8101 atagtgaccc agtagacatg ctagttaatt gtttgaactt aggtatatca acaggattat
     8161 atggtcttac actaactaat cttttaaatg atgttatgtt gggagaacca gttataagaa
     8221 tggctcctgt aggatttaat gtaatagacc cagactacat aaatataatg ataacaggcc
     8281 atcaacattc aacatttgca aatttccaag atagattaaa agatgaagat gttataaaat
     8341 tagctcaatc tgttggtgca aaaggcttta agctagtagg atgtacatgt gttggtcaag
     8401 atttacaatt aagaggagaa cattatcaag aagtatttgc tggacatgca ggtaacaatt
     8461 ttacaagtga ggctgtttta tcaactggag caatagatat agttctttca gaatttaact
     8521 gtactatacc agggcttgaa ccaatagcag ataaatataa agtaaaaatg gtttgtttag
     8581 atgatgttgc taaaaaagcg aatgcagaat acataggttt agacagaagt aaattagatg
     8641 aacttagtaa cactttaata gaaaaagcat tggagtctta taaggagaga agaggaagta
     8701 ttgaaataga tataccaaaa gaccatggat ttgaacaatc tttaactggt gtaagtgaaa
     8761 agaacctgaa agcgttttta ggagattcat ggaaacctct tattaattta attgcagaag
     8821 gaaaaattaa aggtgttgca gctgttgtag gatgttcaaa tatgacagca ggaggacatg
     8881 atgtaaatac tgttgaactt actaaagaat tgattaaaaa agatataata gtactatctg
     8941 caggatgttc tactggagga ttagaaaatg ttggtcttat gtcacctgga gcagaagaat
     9001 tagcaggaga aaacttaaaa gaagtatgta agactttagg aataccacca gtattaaact
     9061 ttggaccatg tctagctata ggaagactag aaatagtagc aacagaatta gcagctgaac
     9121 ttggaattga cctaccacaa ttaccattag tgctttcagc tccacaatgg ttggaagaac
     9181 aagcattagc tgatggagcg tttggtcttg cccttggctt acctcttcat ttagctttac
     9241 ctccatttat aacaggagga aaattggtga cagaagtatt aacagaaaaa ttaaaagact
     9301 taacaggagg acatgttata gtgaatccag accctaaatc ttcagctaat caattagaag
     9361 aaataataat agatagaaga aaaaatttag ggttaaagga tgtggaatgc aatgcataga
     9421 atttttataa ataaagattt atgtacagga tgtaagagct gtgtactagc atgtatgcta
     9481 aaacacaata aagattatga catgtacact cttgatttag aaaatataga taatgatagt
     9541 agagggcata tagaattaga tagtaaacat aataatccag ttccaattct ttgtagacac
     9601 tgtgatgaac ctgaatgtgt attagcatgt atgagtggtg caatgcataa agatagtgaa
     9661 agtggaatcg tttcatatga tgaagagaaa tgtggttcat gttatatgtg tgtaatgtct
     9721 tgtccatatg gattattaaa gccagatgat agaagcaagc agaacatact taaatgtgat
     9781 ttatgtaaag atgaggagta tcctagatgt gtagctaatt gcccaagtgg agcaattgaa
     9841 ttacagaagg aggaaaatga tgaattatgt agtgttaggg gctagtgcag ctggtataaa
     9901 tgcagtaaaa acattaagag aattagataa agattcaaat atagtagtaa tttctaaaga
     9961 tgaaaacgta tattcaagat gtatgttgca tcacgttata tctgaacaca gaattttaaa
    10021 acaaataaat tttgttgatg aagattttat ggagcaaaat aatgtaaaat ggattgcagg
    10081 taagactgtt aaaggtatag acataaataa aaaggtagtt cagacagaag acattactgt
    10141 aaattatgat aaacttttaa tagctacagg tgcatcttct gctattcctc ctataaaaaa
    10201 tttaagagat ggaaattttg tatattctgt gagaaacata gatgatattt ataaaataaa
    10261 agaaaaagca caaaattcta aaaatgtagt tataataggt gctggtttag ttggtataga
    10321 tgcattagtg ggattattta aatatgagca gttaaatatt tctgtagctt ttatggaaaa
    10381 gtacatttta gatagacaat tggatgaata tacagcatca gtgtatgaaa ataagttcaa
    10441 agaaaaaggt gtaaaatttt acccaagtgc atcaatacaa gaaattgtat tggataacag
    10501 taaaaatgtt acaggtgttg cattttcaaa tggtgaagtt ttagatgcag acatggtaat
    10561 tgttgctaca ggggttaaac caaatgctga ctttttagat ggaacaggaa tagaatatga
    10621 tagaggtata ataatagatg atatgtgcca aacaacacaa aaagacatat atgcagcagg
    10681 agatgttgta ggtaaaaatg ctatttggcc tctggcagta aagcaaggaa ttgtagctgc
    10741 ctataatatg gttggaaaag ataagaagat agaagatgaa tttgctttta aaaacagtat
    10801 gaatttcatg gatataccaa ctatttctat aggaatgaat acacctgtag atgatagcta
    10861 taaagtgcta actagacatg gaattaatga ttataaaaag tttgttttta aagataatgt
    10921 aatctatgga gcagttatac aaggagatat ttcatatgta ggtgtattaa cttatttaat
    10981 aaaaaataaa gttgaaatat atgatttaga aaataggata tttgatatat gttatgcaga
    11041 cttttttaat ataaaagaaa atggagaatt ttgttatagt gtatagtata tacgtggagt
    11101 aaggtttata aatacagtaa atcaataaat agagttattc taaaataaga aaaatgacaa
    11161 atcttattta gaaatagctc tattttttgt aattaaattt aaattatatt caatttgtaa
    11221 aatattattt tgaacaatta tacatatact ctctagttag aggtaaatca ttttttatac
    11281 ctttagtgaa aagtatttga tgtatatcta taataccagt gttgaaagca gcagcacaag
    11341 aacaaaggta taaatcccac attcttataa acttatcatc aaacatttct ttaatctttg
    11401 gaagattctc agagaaattt ttttgccagt atagtaaggt ttttacataa tgtaatctta
    11461 gactttctac gtctatagta tgatatttgt aatcactaca aatatgtatc atttctctta
    11521 ggctaggaat aactcctcct gggaaaatat atttttttat ccaagggtct ccataagatt
    11581 cctgcaatcc acttatatag tgaagtaaaa acaatccacc atcttttaaa acagcattta
    11641 cgtttttaat gaaaagttca tagttttctc taccaacatg ttcaagcatc ccaacgctta
    11701 caattctatc aaacataagg tctgattttt ttaattctct atagtccata agtcttactt
    11761 caagatagtc ttgaaggttc tcttctttaa ttcttgcgtt aaattcctta aattgctctt
    11821 cgctaagtgt aattccaacg ccgtgaattt tatatttttt tgcagcttct attagtaaaa
    11881 atccccaacc acaaccaata tcgagaaggc tcataccttc ctctaaattt agtttagtta
    11941 atatgtggtg aactttattg acttgagcat cataaagagt atcattatca tttctaaagt
    12001 atgcacaaga ataactaagt gtcttatcta accataagct ataaaaatcg ttgccaatgt
    12061 catagtgggc atgtatttct ttcttttgat gtctcattga atttgatgag tgcaatagat
    12121 gttttacagc tgaattatca atattgaatt tgtctatttg ttttaaaagt agatttaaag
    12181 ttaagaataa atctccctca acttcaatgt ctctatttat gtaggcttct ccaagtgcta
    12241 aagatgtact ttttaatagt tcagacttgc taatatcatt atttactata actttgaact
    12301 gaggggagcc ctctccaatt agaaattctt cctcatcttt aagcctaact agaaatggtg
    12361 tttcaccaaa tttctctaaa tagtctttta aaaaattttt tgtaatattc atattaccag
    12421 ctccttctaa tgtaaatcca gtaagtttcc ctgcttaaaa tatattatag tccttttttt
    12481 aaaaaagtaa aattttttca ataaattttt tatttaaagg aaatttattt tttataaaga
    12541 aatttcatgt tgtagtatat tttttgctta taaattttgg tactggatat aatattaaac
    12601 tgttaatgat ataataaatt taatctataa agtaaataaa attttgtaat aaatagttaa
    12661 agctatttta taaggagata tatgatggaa gtatatgctt tggttgggtc aagtggcaca
    12721 gggaagagct ttaaggcttt agagttcgca tatgaaaatg atatagaata tataattgat
    12781 gatgggattt taatatacaa aaacaaggtt ttagctggga tatcggcaaa acaagcaaat
    12841 actactatag aagcagttaa aagggctatt ttttataatc tggagcatag acaagaagtt
    12901 agagaagcaa ttaaaaaaga aaatataaga aaaattctta ttattgggac atcaaaaaaa
    12961 atggttaatc aaattgtaga gagattgtca attggaaggg tttgcaagtt tataaatata
    13021 caagatataa gtactaaata tgaaatagaa atagcaaaac aagctagaaa agaaggaaat
    13081 catgtaattc ctgttccagc agttgagata aaaagtatgg caagtggtct tagtataaat
    13141 tctttgaaga gactttttag aaaaggaagt aataggaata tggtagtttt agaaaaaaca
    13201 attataagac caacttttag ttatatagga aagttttata ttagtgctga tgtaattaaa
    13261 cagattattg aatatgaagt atacaacttt ggaagcattg atagaataaa taaaatcaat
    13321 atagagaatt gcaataattt tatgaatatt tttgtaagta taaatatcaa tgatttagaa
    13381 actataaaaa aaattgaaga catacaacag acaataaaaa agtctattga gaaaatgact
    13441 ttagttaatg tacaaaaaat agatattaac attcataaaa ttaaaaaaat aaacttcctc
    13501 caatgttaaa aaataaactg agaaaatgat atactaattt gcaataaagt gtgctataat
    13561 taaactgtaa atttaggaaa tggttttttc taaatggagt atacataatt ataacgtatt
    13621 tataaggggg ctttcaaatg tcaggaaaag atgtaaatgt cttcgagatg gcgcaatctc
    13681 aagtaaaaaa tgcatgtgat aaattaggta tggaaccagc agtttatgaa ttattaaaag
    13741 aacctatgag agttatagag gtttcaattc cagtaaaaat ggatgatggt tctataaaaa
    13801 cttttaaagg atttagatca caacataatg atgcagtagg gccaacaaaa ggtggaataa
    13861 gattccatca aaatgtttca agagacgaag taaaagcttt atctatatgg atgactttca
    13921 agtgttctgt aacaggtata ccatatggtg gaggtaaagg tggaataata gtagatccat
    13981 caactttatc tcaaggtgaa ttagaaagat taagtagagg atacatagat ggaatatata
    14041 aattaatagg tgagaaagtt gacgttcctg caccagatgt aaatactaat ggtcaaataa
    14101 tgtcttggat ggttgatgag tacaataaat taactggaca aagttctata ggtgttataa
    14161 ctggtaagcc agttgaattt ggtggttctt taggaagaac agctgctact ggatttggtg
    14221 ttgctgttac tgcaagagaa gctgctgcta aattaggaat agatatgaaa aaagctaaaa
    14281 tagctgttca aggtatagga aacgttggtt cttatacagt tcttaactgt gaaaaacttg
    14341 gtggtactgt tgtagctatg gctgaatggt gtaaatcaga aggttcttat gctatataca
    14401 atgaaaatgg tttagatggt caagctatgt tagattatat gaaagaacat ggtaacttat
    14461 taaacttccc aggagctaag agaatatctt tagaagagtt ctgggcttca gatgttgata
    14521 tagtaatacc agctgcatta gaaaactcta taactaaaga agttgctgaa tctataaaag
    14581 ctaaattagt ttgtgaggct gctaatggac caactactcc agaggctgat gaagtatttg
    14641 ctgaaagagg aatagttctt actccagata tattaactaa tgctggtgga gttacagttt
    14701 cttacttcga gtgggtacaa aacttatatg gatactactg gtcagaagaa gaagtagagc
    14761 aaaaagaaga aatagctatg gttaaagcat ttgaatctat ttggaaaatc aaagaagagt
    14821 acaatgttac aatgagagaa gctgcttaca tgcattcaat taagaaagtt gctgaagcta
    14881 tgaaattaag aggatggtac taagatttac tcttaataaa taataggata taattattta
    14941 agatataaag gggctgtaca tagtacagcc cttaaattat gtggcttaca atgaagtaag
    15001 ctatttttgt tttaacttat aaaaaacttg ataaattatg taatttagaa tgtatttttt
    15061 accaccacat gattacaaat tagaagagtt agattagatt tttagacatg aacttatgca
    15121 ttttaaaagc aaagacctta atttaaagta tctagtatta tttttaaaaa tagtatattg
    15181 gtttaatcca tttatatata taatggaaaa atatatggat ttggattgtg aactttattg
    15241 tgatgagaga gttttaaaaa attgtactgt acaacaaaag caagaatatg ctctaactat
    15301 tgtaaattca atgaaaagaa gtacaaattt ttcaaataaa tttgtagctg gattaaataa
    15361 acaatctgat attagaaaaa gagtaataaa catgttcagt gaaaaacata aaaatggtat
    15421 cttaagggct ttgattataa gcttgttttc ttctattaca ttttttaaat tcgattcaac
    15481 tagtattatt gaagagaatg atttcttaga gaggtcaaga tatacgtttg aaggaaggca
    15541 agaagacatg tttgtaacaa tagattttat atatgcagat gtacctataa aacttagaaa
    15601 agagcatgaa gaaaactgta gagtcaatgg aatagtacca aaagattcag attcaataga
    15661 aattattcct agattttata aagattgtat ggatttaaaa aaataagtta gacttaatat
    15721 tgatgttgat attatgaatt ttttatattt aagcaattga aaattttatt atagattcaa
    15781 ttttatatgt gtttaagtat gaaatttata gatagttgaa ataattaaat atgattgatg
    15841 cataggaatc attactaagc tataattaat tataagggag tttaaataaa ttatgagtta
    15901 cacaattata aaatgataaa aaatagttat ctgttacaaa aaatgtatct ttttaattaa
    15961 taaagataat atgaaaactt aaaaggcaaa taaacttaaa cataaaatac tcttaaataa
    16021 gataggagaa aaatgtatgt ctaactttat tctaaatggg atttttagaa cagttatagt
    16081 aagtagtcta agcattctta tgatattgat tttcaagaaa aatgtattta aaagatttag
    16141 caaaaaattt aattattata tatggatgat agttgttatt aaattatttt taccatttac
    16201 atattatact tttacaataa atatcttgag aagtaaaaaa cacattaata ttaataacat
    16261 aaatttagag ggatttaata atgtttcaac tataagtaat aatattatat tgtacacttg
    16321 gattataact gtagtagttt atttaattta cactatattt aaatatataa aacttaaaaa
    16381 tttaataaat gacttatctt atgatgttga tgatggagag attgtaaatc tttataaaaa
    16441 tatattagaa gaatttaata taacaaaaga tataaaactc aaatactctt atgaagttga
    16501 aacaccagca ttttttaatt catgtgtgct tttgccacca catgagtata aattgaaaga
    16561 gttagactgg atttttagac atgagcttat gcattttaag agtagggacc tttatttaaa
    16621 atatgtaatc ctgtttttaa aaacagtata ttggtttaat ccatttatat atataatgga
    16681 taaacatata gatttagatt gtgaacttta ttgtgatgaa agagttttaa aaaatcgaaa
    16741 ttcagaggaa aaaaaggatt atgctttaac aattctaaat gcaatgagaa aaggttcaaa
    16801 tacttcaaat aaatttatag ctggactcca taagcaatct gatattaaaa agagagttat
    16861 gaatatgttt aatgaaaagt acaagaatgg tatattaatg gcattaactt tatgtttatt
    16921 atcttctatt acttttttga aggttaattc aacgagtatt gttaatctta atatattgcc
    16981 aaaaggaatg gaagaaaaag atgttactaa tagtgcaatt gttacaatga tagaatttac
    17041 atatgcagaa gctcctaaaa gtatgagaga agaacataaa ataaattgtg aattcttagg
    17101 tttaatacct aaagactctg ataaaatagg tgttggtata gaatattatg acagtctatt
    17161 gaatgattaa ataaatatta ttttaacaga tattaattat aaagttttga atagtaattt
    17221 gttgagaaat aaatatatcc ttataaaaat agatacttat ataccaagaa taaaaaatag
    17281 tcaagatagt acacaaggat atacttataa agaactcaaa taagtgaaat aaattattaa
    17341 ttataaggtt tataatttta aaaaattaga taaattcagg tgtaaaatac tataaaacac
    17401 aattcggaaa agtgtatgtt gaacttcatt taaaatggaa tttcaaagtt gctatagtaa
    17461 gtagtttaag cattcttctt atattaatct ttgagaataa tgtatttaaa aaagttagta
    17521 aaaaatttaa ctattgtata ttaatgatag ttgctattga tatattatct aaaaaaatta
    17581 agtatgaaaa gattactgct aggacaatct tttcatactt aatttttaat atttatatta
    17641 tccaaaattt tcatcttttt aacttagaat ttgagaatca ctattaggaa ataggctttg
    17701 atttatgtag ggttaaaaat ttcacagaat atctaacttt atactaaatc catcaaaaaa
    17761 tatacgccat ataaaagcgc ataaaaaaac acataaaaca atatttatgc gcactttaac
    17821 ctcgaatggt acaaaattct ttacttttac agacccatat tatataatta agtaaataat
    17881 gtatatttag gtgacatatt ggataagcta atttatttat tttattaata taatgatact
    17941 ataaaagtta tataaagtgg tttacaaaaa agttacagat agagctttta ttaatgtatt
    18001 gtgtattagt attttatcct taggtacact tcacctacaa aatagaatca taagataatt
    18061 atgggaaaat tttaggaggt ttatcgtgaa gaaaaagata ttaataccag tatttgcatc
    18121 agttatggca ttatcagtaa gttcaattgt aaatgcagat gaagtaaatg attcatctca
    18181 aaataaggat gataaaacaa atactgaatt aaatttaggt gaatataaag aagtaaaata
    18241 taaagttgca aaaataaaag atggagtagc tataaagata agagaagaag gtcaagttca
    18301 aaacatagct tattctggag atgaatttac ggttttagga actcaaggag aatgggttaa
    18361 ggttaaagtt gaagatggcg aaggttggtt agctactaga tatgtagata tatctgaagg
    18421 tgtaggatat actaacgctg ataaagtaaa cttaagaaaa gataagagtg aaagttcaga
    18481 agtaattgaa gagctggaaa aaggaagttc tttattggtt ttggaagata atggagattg
    18541 gttaaaagtt aaagatgggg aaacagaagg atatgtaaaa tcatcttata tatctgataa
    18601 agctcctgta atagaagagc cacaagtaaa cccaagtatt gaccaaaatg tagattctac
    18661 aaataataat caacaaaaca atgcaaatac taatcaacaa aataataatt caaatgtgcc
    18721 aacagctaac tctaatgcag ttcaagctgt acttaacttg gcatattcta agcaaggttg
    18781 tccatatgtt tggggagcag aaggaccaaa cacttttgac tgttctggat ttacacaata
    18841 tgtgtataga aatgcagtag gaaagaatat accaagaaca tctaaagcac aatctaaata
    18901 tggacaaact gtaagtaaag caaatttaca accaggagac ttagtattct tcacaactaa
    18961 tggttctggt tctgttagtc atgttggaat atatgtaggt gggggtaata tgatacattc
    19021 accatcaaca ggaaaaacag ttagtgtaac tagtataaat tcatcatact atacagcaag
    19081 atttgttaca gcaaaaagaa tattataatc ggtttgtaat atgacaaatt gataaataca
    19141 taaatatata taaacgctct atattatatt taatttaatc atggaaacaa aaaatgatta
    19201 tattttaaat gtactataga gcgtttttgt atcactttaa atctaatcaa gattcaagtt
    19261 aatatgactg ttaaatagga gccttttcat atgatgatta tttgcgcaat cccttgacag
    19321 gatagggcaa aggtggtaag ctactaacag tacaaaaata cttttaattt tagttcagag
    19381 aggctaaaaa aggaggacga aagatgttaa aatcaagaaa tttaatccag ccagaagact
    19441 tttcaataga agaaatagat gaaatattag aattagctca aaaaataatt gacaatccgt
    19501 caaaatattc tcgtatatgt gaaggaaaac tattagctac attattttat gaaccatcaa
    19561 caagaacaag attaagcttt gaatctgcta tgaacagatt aggaggacgt gtagtaggct
    19621 tttcagagcc aaattcatcc tctgcatcaa aaggtgaaac tctaggagat acaatgagga
    19681 tagtatctgg ttatgttgat ataatagcta tgagacatcc tcaatcaggt gcagcttcag
    19741 aagcagctag atatacggaa gtgccattta taaatgcagg agacggcaaa aaccagcatc
    19801 caactcaaac acttacagac ttacttacta taaaatcatt aaaaggaact ttagaaagcc
    19861 atacaatagg attatgtggt gatttaaaat atggaagaac tgtgcattct ttggttaagg
    19921 ctatggcaag atataaaaat acaaagtttg tatttatagc gcctgaagag ttaaaaatgc
    19981 cagattatat aaaagaagct ataaagggac atgcatatta tgaaactaat aatttggatg
    20041 atgttatagg aagcttggat gttttatata tgactagagt tcaacaagaa agatttgaag
    20101 ataaatcaga gtatgaaaga cttaaaaact attatatttt aaataaagct aaattagaaa
    20161 aggcatctaa agatatgtta gtaatgcatc cattaccaag agtaaatgaa atagacatag
    20221 atgtagatag tgatgataga gctgtatatt ttaaacaagc taaatatgga atgtatgtta
    20281 gaatggcttt aataataaaa cttttaggta taaatgaaga ctaatagtta tcatataata
    20341 gtaaaacaaa ggagattttg ggatgtataa aattctggaa aatatatata taggcgaaga
    20401 tatgtataga atgaaagtaa aaggtaattt tgaaggtaaa atgggacaat tctatatgtt
    20461 aagagcatgg gatacttatc cagtactttc aagacctata agtatacatg atatagatga
    20521 agaagggata acttttcttt ataaagtagt aggagaagga actcaaattt tgagtaacct
    20581 aaaggtaaat gataatataa aattagaagg cccttatggt aatggatatg ctaaggttga
    20641 tggaaaggta gctctagtag gaggaggaat cggtgtagct ccactatatt tggttgctaa
    20701 aaatataaaa aattgtgatg catatcttgg gtttagagaa gatgttatat tagaagatga
    20761 atataaacaa gtatgtaata aagtctatac aacagtagga aatacttttg taacagatat
    20821 aattgatgtt gagaaatatg attatatatt aacatgtggt ccaactccaa tgatggaaaa
    20881 attagttaag atggtagaag gtactaaaac gagaattatg gtatctcttg aaaaccatat
    20941 ggcttgtgga gtaggtgcct gtttagtatg tacgtgtaaa actaatggag gaaataaaaa
    21001 gacttgtaaa gatggtccag tgttctgggg agaggacgtg atttttaatg ggtaatttaa
    21061 gtgtgaaatt tggaagtgta gaatttaaaa atccagtaat aatggcgtct ggaacttttg
    21121 gatttggaaa agaatataat gagatttatg atatacaaaa acttggtggt ataagtagca
    21181 agggtcttac tttaaataaa aaacctggta acaatggtat gagagttcat gagacatcat
    21241 ccggtatgat gaatagtgtt gggcttgaaa accctggagt tcaaggattt atagactatg
    21301 aattaccttt ttttagtaaa ctagacttag tcagaatagc taatgttgga ggtgggacat
    21361 tagaagacta cttattaggg gttcaaatgt taaatgacaa gcctatagac attattgaat
    21421 taaatatatc ttgcccaaat gtcaaagctg gtggaatggc ttttggtata aaaaatgaag
    21481 tggcaagaga agttgttcgt gaggtaagaa acataacgaa actgccttta gtaataaaat
    21541 tatcacccaa tgcagaagat attgtaggaa tggcaaaagt ttgtgaagaa gaaggtgctg
    21601 atggagtttc attagttaat acttttaaag caatggcaat tgatataaaa aatagaagac
    21661 cagtatttga aaatgtatat gctgggttat ctggaccagc tataaaacct atagctttga
    21721 gaatggtaca tgaagtgtgt aaaaatgtaa atatacctgt aatgggaatg ggcggaataa
    21781 ctaaagccac agatgctata gagtttataa tggcaggagc tacttgtatc caagttggaa
    21841 ctgcaaattt tatgaatcct agaataggta ttgaaataat agatggaatt aatgaattta
    21901 tggatagaga aggaataaaa agtttagacg aaataagagg aattatataa ggtagaataa
    21961 atagtatttt aaagataaaa aatatagatt aaataaacaa taattttata acattaacat
    22021 ggaggaaaaa taatatgagt aaagtagatg tagtggatat attaaagaaa agtgatgcat
    22081 tgttggaggg acatttttta ttatcttcag gaaaacacag taatagatac gtacaatgtg
    22141 caaaagtatt aagatttcca gaatatgctg ctgaggtatt aagtacagtt gttgaacaaa
    22201 taaaagactt agatatagac ttagtagtag gaccagctat gggtggagta atagtttctt
    22261 atgagttagg aagacaatta ggaaaagaag ctgtatttac tgagagaaaa gacaatacaa
    22321 tggagttaag aagaggattt gaagttaaaa aaggagcaaa gataataatt gctgaagacg
    22381 ttgtaactac tggtaaatca actatggaga caaaaagagt attagaagcc ttaggtggag
    22441 aagttgtagg tgttgcatgt atagcagata gaactaatca tgatataggt atgcctatat
    22501 atagtgctat aaaacttgat attcaagttt atgaatctga tgagtgtcct ttatgtaagg
    22561 aaggaaaatt accagttgtt aagcctggaa gtagagagtt caaggaatta gggatgtaat
    22621 aaaaacttaa ttatttatag tgttacttaa aaaatgtaaa ttttttagag taaaaatttg
    22681 taatataaca tataacttaa aaataaaaag cagttagcct tataaatgaa ggttagctgt
    22741 ttttttgtta aaaatatatt tacaaaataa gagatagtag cagtacaata taagtataag
    22801 gtaaaagtat tatatattag ggaggggtaa gtatgaaaaa tcaattaaat gaaaatcata
    22861 aaaaggttgt attttttgga gtaggtgctg taggagctac ttttgcagaa caatttttta
    22921 actctaaata tgattttaaa attctttgtg ataatgaaag aaagaaaaga tatttagaag
    22981 aaggatttat aataaatgga aaaaggtatg attttgatta tgtaactaaa gatgagtata
    23041 aacaagaggc tgattttata attataggtc taaaatataa taatttaaaa gaaaatataa
    23101 aagaattaga tggattagtt ggaaaaaata cagttataat gtctctgcta aatggagttg
    23161 atagcgaaga gataatagga gaaagatttg gaattgaaaa aatggtatat tcatatgtta
    23221 ccaatataga tgcaaagaaa atcaataata atattataca cactactaat gggataattg
    23281 tatttggtaa caaagataat agtgaagata gaaaaactaa tataataacc gaagtctttg
    23341 atgatgtaaa tatagaatat actttatcaa aagatattca acgagatatg tggtggaagt
    23401 acatggttaa tattggtgta aatcaaactt cagctatact tggtgcacct tatggagttt
    23461 ttcagagttc tgagcattta agagaattag caaaatctgc aatgagggaa gttgttgcta
    23521 tagcacaagc aaaagacata tctcttacag aagatgatgt ggaacattca ttacatagaa
    23581 tactagaaca ttcaaaagaa ggaagaacat caatgcttca agatgtggaa gctcatagac
    23641 ttacagaagt agatatgttt tctaagaata tctgcaaact tggaaaaaaa tataacatac
    23701 ctactcctat aaatcagact ttcttttata tgataaaagt aattgaaagt agattttaaa
    23761 gggtctttgt aagaaatatt gataattatt gattttaaat tttattaatg ttatatacta
    23821 tttaagttac taaatatttt taactcttca atgaaaatag ttgctgcttt tgataaagtt
    23881 atatcttttg gaaatatata tgagaaatgt ctgttgtagc tgcttccaag ctctatgaca
    23941 gacagctcat tgtttagaac aggtaagctt gtcactagat tagagactat tgtaattcct
    24001 aaattatttc taacagattc tttgacagca taattacttc caaaaaccat catacttttt
    24061 ggaataattt ctttaacact taaaaacata tctaaataat ctctggttcc agaaccatcc
    24121 tccctgacta tccatttttg gttttgaagt ttatcaaaac taaaatcttt taataaatga
    24181 ctcttgtatg gaagtgctaa aaccatctta tcttcaaaaa aatattcttg aataaatgaa
    24241 ggggaagagc aagtgccttc aataagacca atgtctaaaa tataatcctt aacatgagaa
    24301 gatacgattg atgtattttt tataaaaact tctacatcta tgtcaggata ctttttagaa
    24361 aataatgcta gaaaatttgg taaaatatac tctccaatag ttaaacttgc accaatcttt
    24421 aagtgaccag ttatagcatc tgaaacttgg cttacatcat gataagtaat atttaaaaga
    24481 tttaatattt ctttagctct tttgtacaag atttgtccac tttctgttat aaaaatggtt
    24541 ttttgtttaa tagacctgtt tataagcaca actttaaaat aattttctag attttttatg
    24601 tgtttactta cacttggttg tgatagattt aaatattccc cagctttagt aaagtttttg
    24661 tattcaacaa cagctacaaa tgtttttaat tcttcaaaca attttttgtc tcctttacct
    24721 aatgaaattt tacatttttt atatactttc tacagataat tatactatat ataataacca
    24781 ttaaaaacat taatcattac gatattatat atttatttta cttatggtta ttacaattat
    24841 atactattgc tatgaagaaa actatattaa ttataaggta ggtatattat gttaaagaaa
    24901 aaaagcagtt tgacattaaa aactataagg gaaatattac caggactgtt tgtatcagta
    24961 ttagtaggtt atattagtat atttatttct attttaattc ctaaggtagg ggcagcatca
    25021 atttctatat ttttaggtat gtttgtagga aacctatttt taaatcaaaa ggtatttcaa
    25081 aaaggttata aattttcaga gacagatttg ttatcatatt caatagtact attaggagca
    25141 acactcagtg tatctacctt aatagactta aaagtttcag gaattttatt tataatattg
    25201 caaatgacaa taacaataat agctgcactt tatataggta aaaaactagg atttgaagaa
    25261 aactttagat ttctaatggc aagtggaaat gcagtatgtg gttcttctgc aataggagca
    25321 acagttccag tggttaatgc gacagataaa gaaaagggaa ttgcagtaac tattgtaaat
    25381 gttactggta tatttcttat gtttttactt ccaataatat ctcaattttt atatagccat
    25441 gaacttgtaa aaacttcagc aatgataggt ggaactttgc aatcaattgg tcaggttgta
    25501 gcaagtggag caattgttgg agaaaatgta aaagatttgg ctactatttt taagatagta
    25561 agagtaatat ttctagttgt tgttgtatta gtatttggac atatgaaaaa tcaatctaat
    25621 agggaaattc ttgaagaaga aaaggatgag ataaaaaaga aaaaagtgac tataccatgg
    25681 tatgtaattg gattctttat aacttgtgca cttttttcaa tgaacattat accaaaagaa
    25741 gtatctgtgt tgtgcaaaga aataagtaac aaactagaaa taatagcttt agcagcaatt
    25801 ggattaaagg ttaatgtaaa agatttagtg aaacaaggga aagaagtttc tttatatggt
    25861 ttatttgtag gtatagtaca agttgtatca gctgtagttt taataaaaat atttatatag
    25921 cataaaaata gtatttgtaa tattaatttg attaactaaa ataatatgat actaataatt
    25981 tataatataa ataaaaaatg aatttcgata agtttaagtg ggtataaact tatagaaatt
    26041 cattttttat ttaaggtaaa aatttaaagt aaaatattat tttacataat aataacctta
    26101 taaattttga aattacttat ctttgtgtta aaataagtaa aatgcacata ttagagctaa
    26161 gtttttaagt aaataaacaa aatgaaaata taaatattaa ggagatttgg tatatgaatg
    26221 tttatgaaaa aggtaatgga gttatactag aaggagtaac agattttgac ccagtacata
    26281 tatttgagtg tggtcaatgt tttagatggc acaaacaaga agatggctca tacactggag
    26341 ttgcaaaggg aagaatatta aatgtcaaaa aagagaatga taaaatatat ttaaataata
    26401 ctaatttaaa ggagtttaac agcatttggt ataattactt tgatttagga actgattata
    26461 cagaaataaa gaataagcta aaaaatatgg atgaatattt aaataaggct actgaatttg
    26521 gatggggaat aagaatatta agacaagatg gatgggaaat gcttatatca tttataattt
    26581 cctctaataa taggattcct atgatacaaa gagcgataga aaatttatca agaaagtttg
    26641 gtaagtatat aggtgaatat gaaggtaatg aatactatgc attcccaaca ccagaagaac
    26701 taaataaggc ctcacaagag gaaataagag catgtcaaac aggttttaga gataagtaca
    26761 taaaaagtac tacacaggca gttatagaga ataatgatga ggtatctgaa tatacaaatt
    26821 taagtacaga ggattgtaga aaagagcttt taaaatttaa tggagtagga ccaaaagtat
    26881 gtgattgtat tgcattattt ggtatgcaaa aatatgattc ttttccagtt gatgtttggg
    26941 ttaagagagt tatgcaagaa ttttatatag atgaagacat gagtttacct aaaatgagaa
    27001 cttatggaat cgataaattt aaagaaatgt ctggatttgc tcaacagtac ttattttact
    27061 atgcaagaga actcggtata ggaaaataaa ttaaggggga gaaattatgg gagtaatagg
    27121 aacaatattt ctgttctatt taattatatc atatctagct ggagccataa tttctgtgat
    27181 tatattgctt gaaaatagag accctgcaaa aacaatgtct tggctattga tgtttattat
    27241 atttccaggt gtaggtttaa tgatatatgc tatttcaggt agaaatatta gaaaaagaaa
    27301 attatttaag actcaaaaac tagctaataa tataaaagaa aagaaactat ttgatacatt
    27361 agaaaagata acagaaatag ttgaattaga aaaagaatct ataaaacaaa ataagctttt
    27421 aagagatgaa gaggatggta gttatagaaa aagggtaata aatatgttgt taaagactgg
    27481 tatgtttccc tttactaaaa ataataaagt ggatgtattt gtagatggaa atgaaaagtt
    27541 taaaagactt atagaagata taagagaagc taaagaccat attcatttag agtactttat
    27601 catcaaagat agtgaaatag gtagggtgtt aaaagaagaa cttataaaaa aggctaaaga
    27661 aggcattaag ataagaattt tatatgatga tgtaggatgc tggagatttt ggtttaatag
    27721 aaaatttttt cgagaaatga gggaagtagg tatagaaatt gcagcatttt tacctacaaa
    27781 atttcctata ataggaggga aattaaacta tagaaatcac agaaaaattg ttgttattga
    27841 tggaataatt ggatatacag gtggaataaa tataggagat gaatatttag gcaaaaatga
    27901 taagtttgga tattggagag atactcatat tagaattaag ggtatttctg tatacatgct
    27961 tcaaatgaca tttctgattg attggtatta tacgacaaaa gaagtattag tgactaaaaa
    28021 ttatttccca agtgtaggga atgttggtga aagtatgata caagttgttg cgagtggtcc
    28081 agacagtgac tgggaagata ttcattatgc ttatttttct gcaatatgtc aggctagaaa
    28141 aaatgtttat atagaaactc catattttat tccagatgaa agtctactaa aagctataaa
    28201 gagtgctgct cttagtggag ttgatgtgag aataatattt cctaaaattg cagaccacaa
    28261 gatagtaaat atagcatctt attcatattt tgaagaaata ttaagagcag gtggtaaagt
    28321 ttatttatat aataaaggat ttatacattc taaagtcgta ataatagatg ataaaattgc
    28381 ttctgcaggt acagctaata tggatttaag aagttttatg cttaactttg aggtaaatgc
    28441 atttatctat gatgaagaag ttattagggt aatgacagat gatttctttg aggatttaag
    28501 tcactgtgaa gaacttaatt tggaggtatt taagaataga aatatcatac aaaaaatcaa
    28561 agaatctgta gcaagattat tttcaccaat attataaaaa attattaaaa aaggtattga
    28621 aaattatgtg tgattggtat attataataa ttgttagcac tctattagat agagtgataa
    28681 taaataaaaa gtatatatat aaatctacat atataagtag tattaattta aaaataacta
    28741 aaaacatagg cgaactagtc tatacttaat attttaggag gcgtgaataa tgaaaataag
    28801 accattagct gacagagtag taattaaaaa agtagaagca gaagaaaaaa ctgcaagtgg
    28861 aatagtttta ccaggagcag ctaaagagca acctcaaata gctgaagttg tagaagttgg
    28921 accaggtgga atagttgaag ggaaagaaat aaaaatggaa ttgacagtag gagataaggt
    28981 tatattccaa aaatattctg gaacagaagt taagatagaa ggacaagaat acacaatact
    29041 aagacagagt gatgtattag ctgttattga ataaatatag aataaattta ttaggagggg
    29101 tttaaaatgg ctaaagaaat taaattttca gaggaaacaa gaagagcttt agaagctggt
    29161 gtaaataaat tagcagatac agtaaaagta acattaggac ctaaaggaag aaatgttata
    29221 ttagataaaa aatttggttc tccacttata actaatgatg gagtaactat agcaaaagag
    29281 atagaactag aagatagatt tgaaaatatg ggtgctcaat tagttaaaga agtagcaact
    29341 aaaactaatg atgtagctgg agatggtact acaactgcta cagttttagc acaagctata
    29401 ataagagaag gtttaaagaa tgtaacagca ggggctaacc caatactttt aagaaaagga
    29461 atacaaaaag cagtaacagt agcagtagaa gaattaaaaa atcaatcaag aatagtagaa
    29521 acacaagagg ctatatctca agttgcttct atatctgctg gtgatgaaga agttggaaaa
    29581 ttaatagcag aagctatgga aatagtaggt aaagatggag ttataactgt tgaagagtct
    29641 caaactatga atactgaatt agatgctgtt gaaggtatgc agtttgatag aggatttgtt
    29701 tctgcatata tggttacaga tgtagataaa atggaagcag ttttaaacga cccatatata
    29761 ttaattactg ataaaaaaat atctaacata caagaattat taccagttct tgaacaaata
    29821 gttcaacaag gtaaaaagtt attgataata gctgaagatg tagaaggtga agcattatct
    29881 acattagtag taaataaatt aagaggaaca tttgatgtag tagcagttaa agctccaggc
    29941 tttggagata gaagaaaaga aatgcttcaa gatatagcaa tacttacagg tgctcaagta
    30001 atatcagaag aattaggtta tgatttaaaa gaagctgatt tatctatgct aggtagagct
    30061 tcatctgtta aggtaactaa agaaagtact acaatagtag atggttctgg agataaaaaa
    30121 gctatagaag atagagtaac tcaaattaaa catcaagtag agcaaactac ttcagatttt
    30181 gatagagaga aattaatgga aagattagct aaacttgctg gaggagtagc tgttgtaaaa
    30241 gttggagctg ctacagaagt agagttaaaa gaaagaaagt taagaataga agatgctctt
    30301 aatgcaacta gagcagctgt agaagaagga atagtagctg gtggaggaac tgcttttgtt
    30361 agtgttatac cagcaatagg aacactaatt gagagtttag aaggagaagt taaactaggt
    30421 gctcaaatag ttaaaaaagc gttagaagag ccattaagac aaatagctat aaatgctggt
    30481 cttgaaggtg ctgtaattgt gcaaaatgtt gttaattctg aagcagaaac tggatttgac
    30541 gcattgaatg aaaaatatgt aaatatgata gaagctggta tagttgaccc aactaaagtt
    30601 agtagaagtg ctttacaaaa tgctgcatca atagcaagta ctttcttaac tactgaatct
    30661 gctgttgctg accttcctga aaaagaagat gcaggaatgc caggaatggg tggcggaatg
    30721 cctggtatga tgtaatttaa aaaatttaaa atataaattt taatataaag aactatttcg
    30781 acttagattt taaactctaa gttgaaatag ttcttttgta tatatctaat ttttgtatat
    30841 ctataaattc aatacacata tttagataat cagtatctag tttgtaataa tattttagag
    30901 tagtattgtt attttttagg ttatgatttt agatatatat taattaaggt ataattgttg
    30961 atagagaata aatcaaattt aaaaggatgc gataatatgg acttaagaaa attgcttgaa
    31021 gaagttaaaa atgaaaatat tgatatagat tttgcgttag aaaagttaaa agatttacct
    31081 tatgaagacc ttggatatgc aaatatagac catcatagag aattaagaaa tgggtatccg
    31141 gaagttattt attgtgaagg taagagtgat gagcatattt tagggattat tgataaaatg
    31201 aatcaaaaaa gttctaacat attggggaca aggtgcagaa aagaaacttt tctgaaaatt
    31261 aaagaaatct ataatcatgc tgagtacgaa gaggcgtcta aaattcttaa gattcaaaat
    31321 catgatatag agaatatagg aaaaggtaaa attgtgattg ctacaggggg aacttctgat
    31381 ataccagtag cagacgaagc ttatcataca gctaagtttt taggtaatga tgttgataga
    31441 atatatgatg ttggagtggc aggtatacat aggcttttaa ataaaagaca taaaatagat
    31501 agtgctagag taatagtagc cgttgcaggt atggaaggtg ctcttgcaag tgtaattggt
    31561 ggactggttg atgttccagt aatagctgtt ccaacttctg ttggatatgg agcaaatttt
    31621 ggaggattag cagcattgct agctatgtta aatagctgtg catctggaat atctgttgta
    31681 aatattgata atggatttgg agcaggatat ttagcatcaa tgataaataa actgtaaata
    31741 cattgatata aattaaacaa aactatataa taaaatttaa tttctattat atttttacta
    31801 ttaaaattga ggagacatat atgagtgaaa aaatacttta ttttgatata ataagcggta
    31861 tatcagggga tatgaccctt gcaagtttat taaatttagg tgtgcctaag gaaatttttt
    31921 tggaggaaat tcataaacta agaatgagcg atgaatttaa tataaacatc agctcgaaaa
    31981 ctgaaaatgg aatagtaggt acaaaagtag aagtaattac aaaagaaaaa catacacata
    32041 gaaatttagt ggatatattt gaggttatag ataaaagcac tctaaacgaa aatgtaaaat
    32101 ctaaagcaaa aaaaatattt atggtaattg gagaagcaga agcaaaagtt catggaacta
    32161 caatagacaa gatacatttt catgaggtag gggctataga ttcaattgta gatatagtag
    32221 gagcttctat attagttgac ttactttgtg tagataaagt ttgtgctagt acagtgtctg
    32281 ttggttcagg atttgtaaaa tgtgaacatg gaattatacc tatacctgca cctgcaacta
    32341 tagaaatttt aaaaggtgtg ccaattaaat taaataatgt aaatggagaa tgtacaactc
    32401 caacaggagc tgcaatcata aaagttttgt gtgatgagtt tgtagatgag cttgaatttt
    32461 ctccaaagca aattggatat ggtataggtc ataagaagtt tgaagtacca aatatactca
    32521 gaactgtatt aggtgaaaaa aaaaagaaga attaatttat gaaattagtg caaacattga
    32581 tgatatgtct tcagaaatct attcttattt gtatgaaaaa atattagatg agaaagcatt
    32641 ggatatttat acagagagca tttatatgaa gaagaataga ccagcaacaa aagtatcaat
    32701 tttatgtagt gaatcagatt tagaaaaatt tattaaaata ttacttttag aaactagtac
    32761 ttttggagtg agatataatg catataatag agaaattcta gagagaaaat ttgtaaaatt
    32821 agatacaaaa tatggagtgg ttacagtaaa actagggtat tataaaggaa aactagtcaa
    32881 ggctactcca gaatataatg aatgtaaaac tatagcaaaa aatgaagata ttcctataaa
    32941 aaaagtgttt gctgatataa attttagaat aaaagaagaa tttagtgaaa acttattgac
    33001 ataagtaata aattttgata aattaatatt cagccttatt aaataaaagt aaacagaaaa
    33061 aaatatataa aatataaact cactcgtata tgctcagaaa tatggtctga gagtctctac
    33121 caagataccg taaatatctt gactatgagt gaaattatta taccaaaaga cctagttaag
    33181 taaatgtgta ggctgagtta aactccctat gtgttggcta taagataaag gtattttgga
    33241 agcctgtata gtaatttcac ttttgctaga aattatggaa gttactatac aggttttttg
    33301 tgtaaaaaga tttaatataa tttagactgt attgtgttaa aataaatata taaaaactat
    33361 attatacaag gagagatgtg aatttatgaa acatgaatta gtacttgtaa tagattttgg
    33421 aggacaatat aatcagttga tagctagaag agttagagaa aacaacgtat attgtgaaat
    33481 acttccatgt actgcaagca tagaaagaat aaaagaaaaa aatcctaaag ggataatatt
    33541 tacaggcggt ccaaatagtg cgtatttaga agattctcca acaatatcaa aagaaatatt
    33601 tgaattagga gtaccaatac ttggaatatg ctatggtatt caaataatgt ctcatgtatt
    33661 gggtggagtt gtaagaaaag gtaataaaca agaaaaagaa tatggaaaaa cagctataac
    33721 ttatggaaaa tcatcattat ttgaaggaat aactacaaat agtgtatgga tgagtcatac
    33781 agatttaata gaaaaagttc cagaaggatt tactatagtt gcaaatacta atgattgtcc
    33841 agtagcagct atggaaaatg tagaacgaaa cctatatgga gttcaattcc atccagaggt
    33901 ggaacactgt ttggaagggg ataagatact tacaaacttc ttatataata tttgtaaagt
    33961 aaaaggtgac tggactacag attcattcat agaagataaa ataaaagaat taaaagaaaa
    34021 aataggcgat aaaaaagctt tatgtgcttt aagtggagga gttgattctt ctgttgctgc
    34081 agttcttatt cataaagcaa ttggggacaa cttaacttgt atatttgttg accatggttt
    34141 acttagaaaa aatgaaggaa atgatgtaga aagaatattt agagagaaat ttgatataaa
    34201 tttaataaga gttaatgcag aagatagatt cttatctaaa ttaaaaggag tttctgaacc
    34261 agaagctaag agaaaaataa taggtgaaga atttataaga gtatttgaag aagaatcaaa
    34321 taaattagga aaaatggatt tcttagtaca aggaacaatc tatccagatg ttatagaaag
    34381 tggacatgga aatgctgcta ctataaagtc tcatcataat gttggtggaa tcccagaaga
    34441 tgtagacttc caagaaattg tagaaccatt aagagagtta tttaaggatg aagttagaaa
    34501 aataggatta gagcttggta tagaagaagg attaatattt agacatcctt tcccaggacc
    34561 aggtctagga ataagagtta taggtgatgt aactaaagaa aaatgtgata tattaagaga
    34621 agctgatgct gtttatatgg acgagcttag aaaagcaggt ttatacagag aaatatggca
    34681 agcttttgca acattaccag atgttaagac tgttggagtt atgggtgatg aaagaacata
    34741 tgcttattta gttggtttaa gagcagttac ttcttctgat ggaatgactt ctgactggta
    34801 taagatgcct tatgatgtat tagaaagaat ttctaataga ataataaatg aagttgatgg
    34861 agtaaataga gtagtttatg atataacttc taaacctcct ggaactattg aatgggaata
    34921 gtaaataaaa aagtcgtaag ccttgtattt tcaaggtttg cgacttttaa aaaaatcttt
    34981 gtgatactaa aatgatacta tatatttaaa ttatctagtt tttgggcaac ttcaagttgc
    35041 ttatttgggt ataaatgtga atatgtattc caagttgttt ctactttttc atgccccaat
    35101 ctttctgcta tagttaatat atttacatcc atatttacca ataacgacgc atgagaatgt
    35161 ctcaaatcat gcactctaat tctttttaca ttagataact tacagcatct atctagctct
    35221 ttagaaagat aacttttagc aaatttaaat attctttcat tatcttttaa gtcatatagc
    35281 ttagatagat aatcttttat attattatat aagaaatgtg ggatagggac aacacgttta
    35341 cttttaggag ttttaggaga agaaacaata tcctctccat ttagtcttat gtagcttttt
    35401 tcgacgctta ttttattttc gaaaatatct tttggagtaa gagctaacaa ctctcctagt
    35461 ctaagacctg tccaaaataa aatttcaaat gctagtctag gttctgattt cttttcaaat
    35521 tcaataaatt ttttaaactc ttctaatgtc caaaagttca tttcatctgc gtttttttta
    35581 ccgatagaac ctgccaaatg agcaggatta gaaggcaagt tataatattt aactgcataa
    35641 tttaatacag caacaagctg gttgtttatc gttttaatat atgtttggct ataatcactt
    35701 tttaataatt cattttgcca tcttcttata tgtgttgcag taatttcatt tatttttaga
    35761 cttttaaaaa aaggtaaaat tctaaagttt attaaatact ttttagtttc taatgtagat
    35821 aattttaatc tagaagacat atcgtgcatg tattcttcta tgagactttc aaaactcata
    35881 tcagtactca ttttagattt attcaaaaat tctctttcaa actctagagc ttctttttta
    35941 gttttaaaac ctctcttaat tttctttttc ctatccccat cgaagtctgt aaaataaaag
    36001 ctagcatacc aactttttct ttgttcgtct ttgtaagcag gcattttaga cacccccaat
    36061 tatgtcattt taatattaca ataattataa catgttagat atgctatcta aataattgtt
    36121 ttattttcct cttcttttta tttcttgaag cttgtaatat ttacacagtt ctaagaagtc
    36181 ttcttctagt gctatagata agtctaatat cgtagatata cctatatctt tgtactcttg
    36241 attttctagt tttgatatat aacttctgtt acgtctcatt ttttctgcta attctagttg
    36301 tgtcattctt ttcttttttc ttaatttttt caacatttat taaaatccac cttttaatta
    36361 tttttatgac atttttataa ttaatagttt gtgtaatttc ttgtaaaaaa tgttcctcat
    36421 tagaacattt tttggtggaa aaatgtgtta aaatgtagtt aagaaattaa aagcaaaaag
    36481 cactataaca atagtgctaa gatggtagag tttaaagaaa aaataaattt tatttaaaaa
    36541 tctataaata caaaatgaac aatttgtaaa ttaaatctgc aaataatgta aaaaaaatgc
    36601 gaaaaaattt tttaaattaa aattatgtaa aataaagtta gtcgactaat taaaaatata
    36661 gataatagag ataatataaa taatacagat aatctgaaac acttggaaac actagagata
    36721 aaaaatagaa gttcattttt aaaatcaaaa ctaaaatctc tatattagtc aagttgcaag
    36781 ataatataaa tttatatata atatatttac aaagaaaatt ttagaaattt taaatattat
    36841 atttgttgaa ataaatacat caagtctata agttaaggaa tttttatacc attttttacg
    36901 aacataagtt ctgaaaaggg atataaaata tagtattata aaaagaaggt gattggctaa
    36961 atattttaca tatttaaggg gcaaggaata aatattataa attataagag ggtgacgcaa
    37021 gtgggaaatt cagattgttc taaaatgttg gcattttatg aaattatgaa aatgttagag
    37081 actattgatg aagctaaatt ttataattgt aaagaagaaa taaagtcaga gtttaagcat
    37141 ataaaaaaag aggattgaat cctctttttt tatatatttt tatgcttatt ctttattttt
    37201 agtattttgc tcctccaaaa aaagttttgc tatctttaaa gctttatttt tagaatcatc
    37261 atcaagttca ctaaaaatat caagtatttc tttcatgtca tcagatggat ttagcttttt
    37321 aataagttct ttttctgtga tattagaaat attaattttg atattagttc tacctaataa
    37381 atagtcagtt gatatatcta aagtatcagc tatatgtctc acaacatcaa gcgctgggct
    37441 tcttttacca gtttcatatc cgttataagt gtttgctggt aaacttaatt tattagccat
    37501 ctctttttgc attaatccga gttcttctct ctcttctctt aatcttttag aaaatatatt
    37561 gctttcatct ttatacattg taattgtccc cctcttaaaa actataacga atgtaattac
    37621 tcttattcta ctacaaaata cgcattttgt gattttaatt cgcaaaaagg aagaaaaaaa
    37681 caaaaaacgt attgacaatt cacaaaacgg agtatattat ataaatatag aaacttcaaa
    37741 ttgagaataa aaggagtgat atatattgaa aaattatata ttgaaatctc ttagagtaag
    37801 acaaggattg agacaaagag atttagcaaa aatattagga atgaatccca gtacttattc
    37861 aagtaaagaa aatggcgata ggcgattcac agtaagtgaa gcaataaaaa tatcagattt
    37921 ttttaataca gatttgagag atattttttt aaataaataa ttcgcgaaat gtgaataaaa
    37981 aggaggtaga taaacatgaa taacttacaa gtaatagaaa gaaataatga gagagttcta
    38041 actacacagc aattagcaga tgtttatgaa accgatgtaa acaatataca aactaacttt
    38101 aatagaaaca aagagagatt caaagagaat gtacactatt ttttattaca aggtgagtat
    38161 ttgaaagaat ttaagaacca acctactaat agtcagttgg tttcaaaaca ttctagtcaa
    38221 ttatatcttt ggactgaaag aggagcaaac agacactgca aaatattgga tactgacaaa
    38281 gcttgggaac agtttgataa cttagaagaa acatatttca aagttaagca acataagcca
    38341 acttgtatag aagatgtatt aatagaaagt ttaaaagaaa tgaaagattt aagacttcaa
    38401 gttaatcaag caaatagcat tgctttagaa gcaaagacag aggttgaaac aataaaagat
    38461 gtagtttcat tagactcaaa tagttggaga acaaatacac atcaactaat tgcaagaata
    38521 gcaaaaaaac aaggtggttt tgaacatata aatatgctta gaacagaaag ttatgaatta
    38581 ttaaataagc gatttggagt tgatttgcat agaagattaa ttaataaaag aagaaaaatg
    38641 gcagaagaag gtgtatctga atctaaaaga gagaaagtta acaatctaga tgtaatacaa
    38701 gatgataaga aactcataga agggtatgtt gctattgtaa aagatatggc tttaaaatat
    38761 ggaatatcaa gtgatttaag caaaaattaa gttaaatcac aacagcactt tgaaaactaa
    38821 atataaaata ttttaaagga gcaagtatat gaaaaataaa gaaaaagaaa tattcattag
    38881 aggaatttta attgaaatct tttatttttt aggaattgta tttagtaata ctttttttaa
    38941 ataataaata gaaggggtag gtgaaatacc aactccccaa atcaaaataa cactttggaa
    39001 gttaaatata gaatattcaa aagaggtgat tagatggaaa tagagcaaac aacaatacgc
    39061 ctgctaaaaa gcagacgaaa gttgaatatt aatttctatt ttggttaggc gaaagaccta
    39121 acaaaaaatc tgtagataca ttaagtttgt tagaaattaa tattaaagtt tctatatttg
    39181 gctctcttga acccgattca taatattgat atgcacgttc tgagatacca aacaaactag
    39241 caaattgctt ttgagtcata ttcatttgct ttcttactag cttaatatta tctttgaatt
    39301 tagtcataac tacacgctcc aataaaaaaa taaataaatt tcttgacacg aacaatatta
    39361 gcgtgtaata tataaaacat agcacgaaca atattggcgt gttaaataaa aaggagaggg
    39421 tgaaaataaa taacaattta aaactacaac gtgaaaaagt tggcttaacg caattagagg
    39481 ttgctcaaaa agccaaaata acagagagaa gctatcaata ttatgaagct ggcgaacgtc
    39541 tcccaaacat tcgtacagct ttaaagatag ctagaatttt aaatactaat tgtgaaaaac
    39601 tctttaatga ataatagcat aaaaggactc agattacaag aaaggagctt tagatatgaa
    39661 taatttgcaa cttaacaata aaaatacaat aacaacatta gaaattgctg atatgttaga
    39721 aatcagacac tgggaagtct taaggaaatt agaaggaaca gaaaaaacaa aaggaattat
    39781 tgatattctt aacgacaaca attttgttgt ggttgattac tttataaaat caacatattt
    39841 ggattcaaaa aatgaaagta gaccttgcta taatgtaaca aaattaggtt gtgactttct
    39901 agcaaataaa tttactggag aaaaaggtat tatttttaca gccagatatg taagaagatt
    39961 taatgaaatg gaacgagtat taaaagaaca acaacctaaa ctaccaacta catacaaaga
    40021 agcattgcaa cagttattaa tagaagttga agaaaaagag caattacaat tagaaaatca
    40081 agaaaaagat aaggtaatcc agttacaaca accaaaagta ttatttgctg attcggtagc
    40141 atcttctaat gactcaatat taattggaga acttgccaaa ttacttaaac aaaacgggat
    40201 tgatactggt gagaaaaggt tgtttgcatg gcttagagat aatggctact taataaaacg
    40261 taaaggtgag gattacaata caccaactca aaaaagtgta gatttgggag ttatagaaac
    40321 taaggaaggt gcaagagttc atccaaacgg atatataagt gtaactaaaa caccaaaaat
    40381 tactggaaaa ggtcaagttt atttcattaa taagtttaaa agtagtaaac agttatcaat
    40441 gttaagctaa atcataacag taccttgaaa actaaataca gaatattttg aaatatatta
    40501 ttaattaatt attaactagg aggttaaatg catgaaaaat aatacaagcg attcaagagt
    40561 aaaatatttt tgcaagtgtc catattgtgg gtttgataat gaggtagaag ttaaaaaagg
    40621 attgaagcct aagatatgtt gcatatgtac aaaagaagtt gagtatgaaa aactggagca
    40681 acaaagtgct ttggaaaata cagaatattt tgaaagtagg tgtattgtat ggcaaaagca
    40741 gtagctaaag aacaattgtt ttatagggca aaagatatag ctaagatttt agatgtatgt
    40801 gaagcaaccg cttataaaat aattggagaa ttaaataaag aattagaaaa agaaggttgg
    40861 aagacctttc ctggaagggt atcagtaact tatttcaaag aaagatattg ttataaaaca
    40921 aaaaaagggg cataaaaaat gctatgagag tagctaaaga tatgtttcct aaagtaattg
    40981 tataaagagg aagggggtga tttagttgag tgtaaaggtg ctaatagatt atgtacagtt
    41041 ttgtaagcaa tacaataaga aagcaagttt tgaaggtctt aaaaaataca ataagggggt
    41101 agtgatatga aagtacttga agtaaatttt cctacagaag tgcttaaagt aggcaaagca
    41161 ataagagtaa catgtgaaag aattagtttt gaaagcgatt gtataataat tcattcacat
    41221 gaatttgaat taacagtagt ctattttgat aaagaattag atgatttagt gcaatcatct
    41281 ataacaatag aagatgcaat aggccatgat tgctgtattg aaatattaaa ttaaggggga
    41341 ttaatcatga aaagtttaat tatagtgaga aatgcagtag agcaacaact aaatagagct
    41401 aatttagaaa taaataaaaa tgaggaactt tatacaaaac ttagaaaaaa agaaaaaaga
    41461 gatatatcag atgaaataga gctaagtaat gctttaagag aaaaaagtgt gaacgaaaga
    41521 ttaaaaatat ttgctgagtc attactaaaa attatagata cacaaattga aataaaagaa
    41581 tatgaagaaa gcgaagatta caagatattt caattaattt cagaagaact tgaaagagat
    41641 aaacctatag atgttcagat ataagaaaag agcctatagc gaggctcaat tcaaaaatag
    41701 aagtaaaaaa tttaattaag ctaattatag cacaaacgga gggaaattat gagtacttta
    41761 tatgaattaa ctacagattt attagaaata gaagaaggtt taacagaaac aacaggaaat
    41821 gaagctgaaa aactagagga aataaaagaa ataataaaaa aagagataca aaataaaaac
    41881 actagaatca tatcagtaat aataaatatt gatagtgata taaactctat agattcagaa
    41941 attaaaagat tacaagagtt aaaaagggtc aaaaagaata ctcttgatag attaaaaaac
    42001 aatataaaag actgtatgga actacttggt actaaaaagg tagaaacagt tttaggaaat
    42061 ataagtataa gaaagtcagc gggtagctta gtcatagaag atgaagaaaa gatacctggt
    42121 atatataaaa cagtagagca agttataaaa gtagataaaa atagtattaa agactttatt
    42181 aaaaaaggtc atgaggttga aggttgcagg attgaatatt caactacatt aataattcca
    42241 aaagctaaaa aagaggtgaa ttaatatgaa taacaaggct ttagaattag caacatgtac
    42301 tttagaatca ggtcaaatac ttgactatac aacagtaaaa aattacttag taagtggaaa
    42361 tggaaatgta acagaccaag aagtactaat gtttatagaa ttatgtaagg ctcaaaaatt
    42421 aaatccattt ataaaagaag catatttaat aaagtttggt aattcaccag caaatatagt
    42481 agttggtaaa gatgtatttg taaaaagagc aaataaaaat cctaactttg agggtatgaa
    42541 agcaggaata gtaactgtaa ataaaaatgg agaagtattt gaaagggagg gcagtttaaa
    42601 gctaccacaa gaagaattaa tcggtggatg gtgtgaagta tcagttagag gaatgaagtt
    42661 tcctataaag tctgtagtgt ctttagaaga gtattcaaag agccaagcta cttggaaaca
    42721 aatgccttgt gtgatgataa gaaaatgtgc aatcgttaca gctttaagag aagcatttcc
    42781 agaagattta caaggattat atgatagtgc agaaataaag actgtaccag ataaattacc
    42841 tcaaaagcct attgaaatag gtaaggcaag tccatcacaa aaacaaggaa tacttaagtt
    42901 agcatcaatg aaaggtttgt atgattatga gaataaaaaa gatacgtcaa agctagagga
    42961 attttgtaaa agtaatggat ttgatttaaa agaactaaaa tttgaagaag tagatgaatt
    43021 aattgaatta ctaagtgaat atgaaccaaa agatgatttt atagatgctg agtttgaaga
    43081 ggtgaaagag gatgaagttg aagtaagaga agaagctgaa aatatagatt gccaaataag
    43141 tatggaagat aatatgaatt ttgaataagt aggtgataga gtgccaattt tcagacagat
    43201 atatacaaat ttttggactg acccaaagat acaagaagaa ttttcagtag aggataaact
    43261 attttatata tacttactaa ctaaccctca tactactcaa attggaatat atacaataac
    43321 taaaaaacaa atagcttttg aaataggttg gactttagaa tctacaaatg cagttatgga
    43381 tagatttatc aatcatcatc aacttataaa ttataattct gaaactagag aaatagcaat
    43441 caagaattgg gctaaatata atctcaatag agcaggaaca ccaatggaga attgtgttag
    43501 aaaagaatta agagagatta aagacaagtc attactaatg ctaatttatg aacatataga
    43561 aaataagaag ttcaaacaga tatttgaaga atattttgac gaactccgta acggtgtacg
    43621 taacgaaact cgtgacgagg ggaataacaa taacaataat aataacaata ataataacaa
    43681 caacaataaa gaggttgcgg tggttgtgga taaaattaaa aaatactttg atttagaatc
    43741 taaggaccta gaaaaaattg ttgatgtatt tatacataca aataaaggga ttgactattt
    43801 agaggaaaaa ttgaggttgg tcaaaaatac ggagagtgta aaaagtgtta caggttatct
    43861 cataaaagca ttacaagaag attataagcc tataccaagt aaacataata aaaataaatt
    43921 tcataacttt aatcagactt ttgaccaata cactgataaa gaattatgcg atatggctaa
    43981 tagaggtcaa cttgaagaaa gtaaattcgg ttaaattaag tattctaggg agtaattata
    44041 caatattact tcctagaagt taaaaaatat tggaggtaaa taatatgaat gaattagtaa
    44101 aaataacgga taaagaaata gcaaaatgta taaagttaca aaaaggattt gcaaagaaaa
    44161 aagattaccc tcattttgcg ccaaaggatg ggatttgctg tagatgtgga agaaatattt
    44221 atcaaaacta tgagataagg ttttttaaag aagctagaat ctcaaaagga tatgctaata
    44281 tagctggtaa agaacttata actggatgcc cacattgcag tagaactttc tgtgattaaa
    44341 aatacaaaaa ttattaggga gttaatataa ctattaactt cctagaagtt aaaaaatatt
    44401 ggagggataa aagatggagt taaaatttag agaatggaat aaaaatggca aagaaatgta
    44461 tagttatgat gaaatgatgt gctattctaa aaatttgctt agagagtggg tttatagtgg
    44521 tgtttattta ccaacaagca atgaaaattt tgaggttatg atatacacag gtttgaagga
    44581 ttgtattaga aaagaaatct atgagggtga tattgtttca tacatcttat catttgaaga
    44641 atttatagga gaggtaaagt ttgaagaagg tttctttgta atagataatg aaatgctagg
    44701 agaaaacgtt gggttatttc atgaaattgc agtggttaaa gttattggca atatatacga
    44761 aaatcctgag atgttagaaa agataagaaa gcctaaagta ttggaggatt agaaatgata
    44821 ataattagaa gtcaagatag attagattta atgagagtta atagagttga aatatacgat
    44881 aaacaagtat ttgtaatatt tgaagatgat gtcaaacgaa taggtgtata tgaaagtaat
    44941 gaaagagcta tgcaagtgtt aaatgatata cagaagttta ttgaaaatgg agtaagaaca
    45001 gattatatag attcttgtag agttagacat aatcaagaga aagtatttga aatgccagtt
    45061 gaataaaagg gggaactaaa tatggctaaa gtttgggtag atgctggaac atttttagaa
    45121 agaactaagg atatagaaga tatgtttgaa tttaatctaa gaaaagtaag agatagaaat
    45181 aaaaaagcaa atgtaattga tttgcgtgta aatgatgagg ggcaaggaag aaaaggtaaa
    45241 aaagtagagt gttttaatat tgtgattgga gaaactaaag tatttaatac tgctgttgaa
    45301 gcaagtaaat acgtatattt tacagatgtt tatattgctc atttagcaag aacaggaaag
    45361 actactaaaa atggctggaa agttagatat attcaagagg tggcagatgg tattagcaaa
    45421 tgtggaacaa gtaattaagt tagctgaaaa gatattaaat aagaaaaagt gttctgttaa
    45481 taaagctatt gatatagcta taaaaatatt aagtaaatat gagtgcgagg ggatgattag
    45541 aaatgagttt aattaagtac agaggttatg attttgagaa tgagaagtgg atttattctg
    45601 aaacaataaa gtggagtgat gcagtagatt gtttatttat gctaaaagag aattgcgaat
    45661 ggcaaaaagt atctaatgtt ggagtattct ctaaatgttg gtctggaaat aatgaagaaa
    45721 tttatgaggg agatatattg aaagaaccct ataatactaa aaataaatat gaatatggaa
    45781 ttgtaagaca agaaaattat gctcttgaat tatatgttga atggcattat ttaaagcaat
    45841 ttgaaggtaa atgggaggaa attacaagta aagcaactat aatgaatagc aaaaaatatg
    45901 tagtagttgg taatgaatgc gagaatttgg aagaagttag aaaagaattt ttagagagta
    45961 aggagaaact tgagaatgaa aatacaaatg cagttaacta aagataaaga gagtttcaaa
    46021 gtttgtgtaa attcagaaga agaagagtta gagaaattgt ttgatgaact tgtatcacaa
    46081 atgttagctt gtagaagaaa aaataagaaa gttcaaggag atattgagaa atgaaattaa
    46141 aagatattat aaaacttgga gaaaagtatt gttattgtcc tcattgtggt aatgacaaga
    46201 ttggaagtaa cgaaggcaaa ttaatagttg aagaacacac atattataga gaatgttctt
    46261 gtggatttag tatattgatt gatgatagaa aggatgagat ataatgaaca tcttggctag
    46321 tgtgctactg ataggaatta gttttgttgt tggtagggtt tatgagtata gattgaatct
    46381 gaaagagtgt gaaaattatg ataatatagg aggttttaag aatgaatgat agattagaaa
    46441 tgataaatgc ttctgtaaat tatatacaga tgatatgtga aagttcaaat atagctatta
    46501 tagcagagca tgaccgagtt agaatattgg atttagaaac taaagaaaag tatgacctat
    46561 taagaagtga gggggattaa aaaatgagta aagttataca atgtgatttc tgtaaggaaa
    46621 tatttgaaaa atataatgga gaatgtattg agctatataa aaaggattgt agcaaagggg
    46681 tgcttgggat agataaacac atatgcccaa cttgttatga aaaattcatt gaaggaaaaa
    46741 tagagaaagt agaaaatgct aaaagattta aggataaatt aatagacttc ttggttgagc
    46801 atgaggtttg tgactgttgt gcttgcgact gtgtgtttga ttctaatgac gaaaaaaaat
    46861 gcaaggcagg aattagagag tgggttgaaa gtgaggtaga agaataatat ggcaaaatta
    46921 atagttagca tacctttaac taaatattta aaagatataa ctaaagaatg gggagaaact
    46981 tatataacta aaacatatgg tgggcatgtc tatttaagtg atgaagctcc aggagaagta
    47041 tttaaaaaag aagatgattt aaattatata gagtttaacg attctgatat tgtcatgaga
    47101 ttacctaaag aagtacatca acatataaat ttgaaaaaag gttgcaacaa gaatcttaaa
    47161 gatgtattaa aagaaataaa aaagatagaa gagaaaacaa atatatagaa aatatctaat
    47221 taaaacagtt tagagagttg caaaatatct tttagtataa tttattgttg aagtgttttg
    47281 tgactctcaa aaatgaaata aaggaggcgt tgtattgctt acatttttag atttattcgc
    47341 agggataggt ggctttaggc tagggatgga aaaagcagga cataaatgtt tgggacattg
    47401 cgaatatgat aaattcgcaa atttaagtta taatgccatg cacaaaccga aggaggatga
    47461 atggtttgaa agagatatta gagaaattag aacagaaaat atcccaagag cagatgtctg
    47521 gtgttttgga ttcccatgtc aagacatttc tgttgcaggg aaacaatttg gattcagagg
    47581 agaacgttca agtttatttt ttacagttac aaaacttatt agagaactca aagaagaaga
    47641 tagacccaag tatttactta ttgaaaacgt taaaaatcta cttagtgtta atggaggatt
    47701 tgatttcctc aaagttctcg ttgaactgga tgaaatcggc tatgatgcag agtggcaagt
    47761 tcttaattct aaaaacttcg gagtacccca aaatagagaa cgaatattca ttgttggaca
    47821 ttttagagga cgaagtacac gaaaagtatt tcctatcgaa agaaaaagtg gaaaaaatct
    47881 tgagcaacta aataatccaa ctcatagtac aaatagaatt tatgatgcag ttggaattgc
    47941 tagatgtatt agaagtcagg caggaggtgg aggtgctaaa acaggtctat actttataga
    48001 cttaaataaa aactctaaag taacaataaa tgctagatgc cttaaagcaa aatataatgc
    48061 aggtgtgaca aatagaaatt gtgataatag tggagtttta gttaatgcag ttttaacgcc
    48121 cgatagggta aataaaagac aaaatggtcg tagaattaaa gaaagcggag aaccaatgtt
    48181 cacattgaca gctcaagata aacatggaat tttgaaaaat ggagatataa gaaggttaac
    48241 accaaaggaa tgctttaggt tgcaaggatt tccggataaa tattacgaaa gagcagcaag
    48301 tgtatgctca gatagtcaac tgtacaagca agcaggaaat gctgttactg caaatgttgt
    48361 atatgaaata gcaaaaagaa tgggctaaaa gttgcaaaat gtcttttagt atgaatattt
    48421 ttgaagtgtt ttgtaactct caaaaatgaa aataaggggt gggataataa aatggattta
    48481 aataaaatta tgaatgatgc actagtggaa atagaagaaa gtggctttgt agaggaaaca
    48541 gttaaaaaag aattagaaga aacaataaaa agagctgtga atgaaatctt tggaagctat
    48601 agtaaattca gaaaaaatat tgaacaacat ttaggtgaaa atataaatat aaatttagat
    48661 gaattagata tacaaaaata caatttactt gtggcaaatg ttgtaaaaga aaaggtagat
    48721 gctacgatga aggggcaagg tattgagcat ttaaaaagaa acttggataa catgcttgta
    48781 ggtcttaaga aagaatacaa aatgagtgaa ttattagaag aattgaaaga agataaatac
    48841 aaccttgacg agtacccatg tgatgaagac tgtataacat ttatccttga atctaatttt
    48901 aattcagaat gggcaagatg gatagacttt gatgagtgtc ctaatgtaag cagaaataat
    48961 tgtgaacatt ctatactatt aagaaacgat ggaactattg cagcatttag ataccaaaac
    49021 agagaaatat catcaaaaga tataatgaac ggatttggta attttgggga tttattattt
    49081 aagatatatg cacataattc aaaaattatc ttagatttag ggaatgatgt agacgattat
    49141 gacttaacaa atggagaaga ttattaaaat aggggatgga acaaatgtca aagtatgtat
    49201 tgagatggca aaaaggacta ttactagatg aacgcaagat aaattattca tgtggaagta
    49261 aagaaatgtt aaaacaaaaa gcagaactat tagctaaaga tgacaagata ctgcttgtaa
    49321 caatagataa agttgaagaa gttataaaag atactagaag tcaaaaaatg gctgaatatt
    49381 attgtgatgg aggaattgaa atatgataat acacaaattt ataatacatg ttttagataa
    49441 gaatagcgat acaccaatac taaatgattt tgagggtagg gttagtcaag atattgaagc
    49501 tttctttcaa aagaaaataa gcaaagtatc aagagataat gacatcagaa cagcagtatt
    49561 taatgactat agtaacaatc taattaagaa gtgttgtgaa caaattat
//
LOCUS       DAMJYR010000015        49111 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.015, whole genome shotgun sequence.
ACCESSION   DAMJYR010000015 DAMJYR010000000
VERSION     DAMJYR010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 49111)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49111)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49111
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.015"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            67..1626
                     /locus_tag="PC471_000849"
     CDS             67..1626
                     /locus_tag="PC471_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDF2787989.1"
                     /translation="MKFKKLASLILVSSLMLTFTACASNDKDKSSGSKSGGTVVIPMA
                     SDPDIINGAFANVKEDSLASNIVYAPLYTYEKGNLVNYLAEKVDFKDSKELTIKLKSN
                     LKWHDGKPITAEDVLFTFNTVLDEKQNSPSRQYLLVGEKPVKVEKIDDLTVKITLPTA
                     SESFLYGISKISPIPKHVFEGESNIAKSEKNNNPVGSGAFKFKEWKKGESIVFEKNAD
                     YFGGESKADSIALKIIPNEASQEAALNNGEISLMKTSAEGYEKAKSNSNLQTYTYSEE
                     RLNYIVFNQNISNMANKEVRQALSYALNRNEMIESAYGKEGSVPAKSILVPEADFYTE
                     EGVEGYDQDTNKAKDLLDKSGVKIDKLKIGYNTGRFGHKNYALVAQQELKKIGIEAEI
                     VPYESKAFFNILFSNSTECDMYVNGYAWGLEPNPYRGMFETGQYCNQTKYSNAEIDAL
                     WEKGFTELNKEKREEIYKQIQQDISKDAPIYTIDYEQNLMAAQKNLKGIKDAKPSPAI
                     LFEDWSKLYVE"
     gene            1732..2691
                     /locus_tag="PC471_000850"
     CDS             1732..2691
                     /locus_tag="PC471_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2787990.1"
                     /translation="MLKLILNRVKVLIPMLILISILSFGLLELAPGDPADAYINPLMS
                     SQDIENIRVNMGLDKPAYIRYLNWLKNTLSGNLGISYINHMPVTEQIMEKMGNTFILM
                     GTSLIFSILVAIPLGIFLAVNKNSITSKVSSVFNYIGVSIPSFWIGMILISIFSVKLN
                     IFPSGGMHTIGNDSIGDLAKHLVLPVITLGLYNTAIFTNYVEASVNEQLKKQYVITAR
                     AKGLSEKVILFKHVLKNSLTSLVTILGMSIQKLVTGAFVTEVVFSWPGMGRLMIDSIF
                     SRDYTVIMAITMLSALFLILGNLVADILYLLIDPKIKSSKGGF"
     gene            2694..3506
                     /locus_tag="PC471_000851"
     CDS             2694..3506
                     /locus_tag="PC471_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2787991.1"
                     /translation="MFTSILKKLKKDKSFAFACITLSIIIILIIASFILNIDSNAIDT
                     TSKLQEPSLKHIFGTDELGRDYFSRALYGGRISLSVGILSMLISVVFGTAVGVISGYI
                     GGKLDQFLMRLVDMLMSIPSFLVLIVLNTYLTPKVSTIILIIGFLSWMEVSRIVRGET
                     LKIKENEYCLAAKATGIKTRNILLRHIVLNLKETIVVAGSLNIANAILTESALSFLGL
                     GIQLPLASWGNMLQDAQRHIFDKLYLAVFPGLLIFLTVMSLTIISKKFSSNT"
     gene            complement(3722..4624)
                     /locus_tag="PC471_000852"
     CDS             complement(3722..4624)
                     /locus_tag="PC471_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDF2787992.1"
                     /translation="MDIKQLKYFVEIVKSGFNLSIASKTLHISQPALSQIIKNFEESE
                     DVYLFERYKGRLNGLTPAGERFYVNAEHIINEYKNMMEDLREDSVQFKGKVRIGIPPL
                     ILGIAFSDVVAQLVANNPDIEFDIVEKGAYDLNRMLILQELDYAVLLHPHKIDKSVVT
                     EHVLQEDELTAFLNINHPLAKKDKIDWNDLNEQLLAIFNPTFMIHHKLMQKFTDENVK
                     LKRYIMSGSWDFLLLSTTNSDFITILPSPVHDFFENSKIIEKPFNHPITWKVILCRPK
                     KERYSHVDQHVFKFIIDFFSKKNS"
     gene            4979..6157
                     /locus_tag="PC471_000853"
     CDS             4979..6157
                     /locus_tag="PC471_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000786547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HDF2787993.1"
                     /translation="MKDVVIVSAVRTPIGSFGGVFKNTSAVQLGTIAVKEAISRVRLN
                     LSEIDEVIIGNVLQTGLGQNVARQIAINAGIPNSVPSYTVNKLCGSGLKSVQLAAQSI
                     TSGENDVVIAGGTENMSQAPYIVPTARFGSKMGDITMVDSMLTDGLIDAFNQYHMGIT
                     AENIATKFEFTREMQDKLALESQNKAENAIKNNRFKEEIVPVDVLIRRGKIETIDKDE
                     YPKLGMTFEGLSKLKPAFKKDGTVTAGNASGINDGAAMLILMSQQKADELGIRPLAKI
                     KSYASAGVEPEVMGTGPIPATRKALKKAGLSINDIDLIEANEAFAAQALAVKNELQID
                     SSKLNVNGGAIALGHPIGASGARILVTLIYEMQKRKVETGLATLCIGGGQGISMVVSR
                     "
     gene            6166..6822
                     /locus_tag="PC471_000854"
     CDS             6166..6822
                     /locus_tag="PC471_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit A"
                     /protein_id="HDF2787994.1"
                     /translation="MNKIVSIDEALSHVKDGDTIMVGGFMANGSPENLIDYLCSKNIK
                     DLTLICNDTGFIDKGVGKMVVNKQFKKIIASHVGLNKETGRQMNNNETDVELVPQGTL
                     AEQIRAAGYGLGGILTPTGIGTLVEEGKQKIVVDSKEYLLEKPIYADVALLFASKVDK
                     AGNLVYKGSMNNFNNLMASAAKITIVEADEIVEIGDIDPNEVNTPGIFVNYIVEGGNL
                     "
     gene            6822..7487
                     /locus_tag="PC471_000855"
     CDS             6822..7487
                     /locus_tag="PC471_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacid CoA-transferase subunit B"
                     /protein_id="HDF2787995.1"
                     /translation="MDKLEMQEYIANRVSKELKDGAVVNLGIGLPTKVANYIPDNVNV
                     ILQSENGFLGLGSAEDGNSSDETIVNAGGQPVTILPGGCFFDSATSFGIIRGGHVDIT
                     VLGALQVDKYGNIANYMIPGKMVPGMGGAMDLVTGAKKVIVAMEHTSKGSAKILNNCT
                     LPLTATNAVDLIVTEMGVMEVTSDGILLKEINPAFTLDDVISATEAPLILSDSLNGKI
                     SVV"
     gene            7592..8368
                     /locus_tag="PC471_000856"
     CDS             7592..8368
                     /locus_tag="PC471_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyrate dehydrogenase"
                     /protein_id="HDF2787996.1"
                     /translation="MVKDKVVFVTGAASGIGKQIAESFLKNGAKVMFSDINQEALNNT
                     ITELQKKGYDCMSVKCDVTKEEEINHAIDKTVEKYGRLDILINNAGLQHVSMIEDFPT
                     DKFEFMIKIMLTAPFIATKKAFPIMKKQGFGRIINMASINGVIGFAGKAAYNSAKHGL
                     IGLTKVAALEAADSGITVNALCPGYVDTPLVRGQFNDLSKTRNIPLENVLEEVLYPLV
                     PQKRLLDVQEIADYAMFLASDNAKGITGQACLLDGGYTAQ"
     gene            8430..9764
                     /locus_tag="PC471_000857"
     CDS             8430..9764
                     /locus_tag="PC471_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HDF2787997.1"
                     /translation="MSIIAPIGIIIGIIAIIYFSVKEIHITIAAPIATLIVVILNKMD
                     VVTSMLGADKNNYMGALGNYIMSYFAIFLLGSILAKFMEESGATVSIADFILNKFGSN
                     HPYRVLVAIFIVSFILTYGGISLFVVMFAVIPLARTLFKKLDISWNLIQIPLWLGIAT
                     ITMTIIPGTPAIQNVIPIQYLNTSLTAATIPSIVGSIGCATFGLVYMKYALNKSIRKG
                     ETYATYTSELEEESLDRELPGFFASIFPLLALVIIAITGSTFGSEFMKKNVIYIALIV
                     AILLSAFLFHKFLSSKIQTLSIGASGSIGPIFATSSAVAFGAVIMAAPGFDVFSKLIM
                     SIPGSPLISLTVLTSTMSAITGSSSGALGIVMPNFADYYLSTGLNPELIHRVATIASN
                     ILTIVPQSGVFLTFLSLSKLTHKTGFKETFIVVAVGSLIAEIIVITLGLLMY"
     gene            10260..11192
                     /locus_tag="PC471_000858"
     CDS             10260..11192
                     /locus_tag="PC471_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein"
                     /protein_id="HDF2787998.1"
                     /translation="MMRRNIPTKLKTILALGVLTLATASSAVTASASSLENNVNISNK
                     SLVYSNTKYKFSAKPNCSVKPELKPDKDNSCKDKNHNDKNCNNTNKPEDNNNSDSTNK
                     PDNNKPESNKPEDNNNSGSTNKPNNNKPESNKPEDNNNSGSTDKPDNNKPESNKPEDN
                     NNNNSDSTNKPDDNNNSGSTSESFSAYQKEVVDLVNIERAKAGLNPLTLDSSISNVAT
                     KKSQDMIDNNYFSHNSPTYGSPFDMLKKFGISYKTAGENIAMGQKTPKEVVNAWMNSE
                     GHRKNIMNPNFSKIGVGVAQKSGGSIYWTQIFVG"
     gene            complement(11584..15123)
                     /gene="nifJ"
                     /locus_tag="PC471_000859"
     CDS             complement(11584..15123)
                     /gene="nifJ"
                     /locus_tag="PC471_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009900447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HDF2787999.1"
                     /translation="MAKFMKTLDGNTAAAHVAYAFTDVAAIYPITPSSTMAEVVDEWA
                     SQGRKNIFGQTVNVVEMQSEAGASGTFHGSLQGGALTSTFTASQGLLLMIPNMYKVAG
                     ELLPGVFHVSARALASQALSIFGDHQDVMAARQTGCVLLASGSVQEVADIAPVAHLAA
                     IEGRLPFIHFFDGFRTSHEIQKVELLENEDYASLLNFEAVQAFRDNALSPNHPVARGT
                     AQNPDIYFQTREASNKYYQNIVGIVEKYMEKMSNLTGRKHSLFDYYGAEDAKYVLIAM
                     GSVTETIEETIDYLNAKGEKYGLVKVHLYRPFSMKHFLDAMPSTVERICVLDRTKEPG
                     STGEPLYLDVRDVFYGKENAPMIIGGRYGLGSKDTTPSDIKTVFDNLVSEQPKNGFTV
                     GIVDDVTNTSLTPSEPIKIASKGTIRCKFWGLGSDGTVGANKQAIKIIGDHTEKYAQA
                     YFDYDSKKSGGITMSHLRFGDTPIRATYLIDEADYIACHKQSYVYQYDLLKGLKKGGT
                     FVLNTIWDQAGLEEHLPAHMKQYIAKNDIKFYTVNAVKLGQEIGLGNRINMIMQSAFF
                     KLAEIIPEEDAVKYLKDSIVKAYGKKGEKIVNMNYAAVDAGINALVKVEVPASWENAV
                     DADKGEVKEPEFIKNILRPMTAQEGNNLPVSTFNGIEDGTFPCGTAAYEKRGIAVDVP
                     EWIIDNCIQCNQCSFICPHSCIRPVLVTEEELANAPEGFEAKKALGKGLEGLKYRMQL
                     SPLDCTGCGNCADICPAKEKALVMKPIDTQLDTELDNWAFAVNPDEVAPKGDVMPVNT
                     VKGSQFRQPLMEFSGACAGCGETAYIKVVTQLYGDRMMIANATGCSSIWGASAPSIPY
                     TCNHEGKGPSWANSLFEDNAEYGFGMYTAVKQIRNKIVDAMTELVSMGICEDAKAVFT
                     EWLDSRNDGEASKVASAKVVELLEKPACDCTDEKAKELVKAIKDRKDYLVKRSQWILG
                     GDGWAYDIGYGGLDHVLASGEDVNVLVFDTEVYSNTGGQASKATPAAAMAKFAASGKK
                     SRKKDLGMMAMTYGNVYVAQVAMGADKNQFIKAVIEAEKHDGPSLIIAYAPCINHGLK
                     EGMGRTVANEAQAVACGYWHLYRFNPEVKAEGKNPFTLDSKEPTASFKDFILAQVRYS
                     AIAKQFPEEADGLFAKAEADAKERYEGYKKLAE"
     gene            complement(15641..16210)
                     /locus_tag="PC471_000860"
     CDS             complement(15641..16210)
                     /locus_tag="PC471_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate propanoyltransferase"
                     /protein_id="HDF2788000.1"
                     /translation="MKLPIALSNKHVHLSQADIDVLFGAGHELTKFKPLSQPGQYACE
                     EKVDLVGPKGTLKGVRVLGPARAHTQVEISLADGFPLGVKAPIKESGDIAGTPGVKLV
                     GPAGEVEMKEGVIVASRHIHMSNEDAAKFGVTDKQIVKVKTSGPRALVFENVLVRASD
                     SFNLEMHVDMEEGNAAGVKNGDLVELIVD"
     gene            complement(16282..16890)
                     /locus_tag="PC471_000861"
     CDS             complement(16282..16890)
                     /locus_tag="PC471_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HDF2788001.1"
                     /translation="MDSNDKKGLKSPEIIIINGSPRINKNCSFIANEVKAMLEECGLS
                     CKIFNIYNMDIEYCTACGFCEKTGYCKFNDDMKPLYKMFDDSKGTIVISPVHFDSISA
                     KLKTVVDRTQAIYASKYVLNKPSIDRSKKRIGMYIAVGGSKSYDSQFKGGQIVMDFFF
                     KSINTKLLHNIYLNNTDDVSYIDNQEFKDIIYKNLKIYIDSI"
     gene            complement(16898..18661)
                     /locus_tag="PC471_000862"
     CDS             complement(16898..18661)
                     /locus_tag="PC471_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HDF2788002.1"
                     /translation="MTNGSYKMKNLVEISAMVTESTDFFNIKDDIIEKMLEVVHPTKA
                     CVNLFYKNDSKYAYLVCSQTLEYVPQIFPINSLKGAKIDFDTYPEYIHEAVNEKKIIY
                     VENVFEDSRAEGERDLAKAEGYIGRIVFPFIINNVVVGFMTCFLKEDDYITEQDIDFI
                     SSVASLLSLSIEITNKNNNVQILIDKLRGSISAINEATKKLYLNKSIDGFLENLSKQA
                     CNITNSKEALIIINSHHNKDAIVSCYNVEQKKKTNLYPMIDMFLKKESLGGYLNNLKP
                     CSKKGINLESYIYYKLQDKNDTIGCIVCANSKNYTSDDLNILSILSKQVSVGMQLYEH
                     NEKEVKHKVLENELNILNKQQQLIMDKGKMDCNDEKELYFYHKPARVVGGDFYYATKV
                     DDEHIVYIVADVMGHGMVSNYVVAMIKGAFKVLCNQYNTPREIMTNLNRMLYDEFDKM
                     GVFTTCLIGIIDTKENMITVSNAGHYSPVVVKKDGTIENNLNCKKGIPIGIMEDATYE
                     NNTLSMEDYAMVCMYTDGILEIKNSSKEEYGINRLENFLKENFRLNQQLIVENLKLDL
                     KNFSSKDNYDDDILIVMLKDR"
     gene            complement(18704..19171)
                     /locus_tag="PC471_000863"
     CDS             complement(18704..19171)
                     /locus_tag="PC471_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2788003.1"
                     /translation="MKMLNGIIKLFYSEIVKTKINNLDSSGYSLRDECKEIRNITLKI
                     DSVFFKLLKLAFGSILLMVAFNLNMVLGIGVFLVGVVYILYKRNMEEQVKEHINNVKN
                     NIDISKITFMDEKGRTGLNALITLLLIGLLTGFNWILVASFVVVFVFTIKNVC"
     gene            19355..19816
                     /locus_tag="PC471_000864"
     CDS             19355..19816
                     /locus_tag="PC471_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2788004.1"
                     /translation="MQIEQLDLETRNKIYCYTKKILRKYQKGITSGKLTADKFADNIL
                     SQDFISSILNEKIVNETNFKISYRNYIETLINIQNENLSNLRKKSTKTAKCFNISQIT
                     QLKNLLSNTGYNLLIPYKYLTARDIEGIVTLINTGSIELGNERIYNYISKV"
     gene            complement(19909..20079)
                     /locus_tag="PC471_000865"
     CDS             complement(19909..20079)
                     /locus_tag="PC471_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2788005.1"
                     /translation="MSSVNFEDLKNKFINSDLDEKIRIYTTTEGLSVEQFKELLKYYP
                     IQHLSKLEKALG"
     gene            complement(20268..20489)
                     /locus_tag="PC471_000866"
     CDS             complement(20268..20489)
                     /locus_tag="PC471_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000002841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta-type small acid-soluble spore
                     protein"
                     /protein_id="HDF2788006.1"
                     /translation="MASNNNNNRTVVPGAKEALNQMKLEIANELGMSNYQQVDKGNLT
                     ARENGYVGGYMTKKLVEMAEQQMAGKSNR"
     gene            complement(20573..21799)
                     /locus_tag="PC471_000867"
     CDS             complement(20573..21799)
                     /locus_tag="PC471_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HDF2788007.1"
                     /translation="MKKVVIIGGGPAGMIAASTACEKGYDVTLIEKNHKLGKKLAITG
                     KGRCNITNACEIEELIENVPTNGKFLYSAFYTFTNDDVISMFNNLGVKTKTERGKRVF
                     PESDKAFDIVNALERQLKSKKVNILLNSKVEKIISKNNKIEKVILNDKKEIKCDSVVV
                     ATGGLSYPLTGSTGDGYKFAISQGHTIIDTKPSLIGIEVQESFTKDLEKLSLRNVEIR
                     VFNSKQKKVYSDFGELEFTKFGLDGPIIKSASCRMKDTRKENYTILLDLKPALDEEKL
                     DKRVQKDFQKYTNKKFEKALDDLLPKKLIPIIINLSEINPNTVVHQISREQRKNLVHL
                     LKNLKFTVKRYRPIEEAIITSGGVKVNEINSSTMESKLVEGLFFAGEVIDIDAYTGGF
                     NLQIAFSTGYLAGFNC"
     gene            complement(21861..22793)
                     /locus_tag="PC471_000868"
     CDS             complement(21861..22793)
                     /locus_tag="PC471_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HDF2788008.1"
                     /translation="MDIQLNILLFFQNLRNPILNFIFLVFTISTEAPLLILITTIIYW
                     CINKKCGQKILFAIIGNFVVNLGIKEFVKAPRPIGIKGLESLRVSTAGGYSFPSAHTQ
                     TATTFWVSIMTIFKKRYLHLIGAVMVLGVGLSRLYLAVHWPIDVIAGWILGIFFTVVF
                     VKIFDYIDDNKNYILLVVLLIPFIIVAIFLNSPEYFEKFGIIVGFVFGYMVEDRFVKF
                     NTDNNNKKINFSNKNKGSKNIIFSRICRFLVGIVTIGMLYIGIKLFISMLIVTLNISD
                     STMSIIFMNFIKNTVVVFYGIAGAPALFKLLKLN"
     gene            complement(22882..23409)
                     /gene="hpt"
                     /locus_tag="PC471_000869"
     CDS             complement(22882..23409)
                     /gene="hpt"
                     /locus_tag="PC471_000869"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HDF2788009.1"
                     /translation="MYKVTGKMLTEEQIKEKVYELGKKIEEDFKGEDLLVVGILKGAS
                     VFVSDLIRCIDLDVNIDFMSVTSYGNSTESSGTVKILKDLDVDIEGKNVLIVEDIIDS
                     GLTLSNLVAALKTRNPKSLKLCTLLDKPQRRKANIPVDYVGFVIEDKFIVGYGIDYAE
                     KYRNLPYIGIVEDVE"
     gene            23638..24714
                     /locus_tag="PC471_000870"
     CDS             23638..24714
                     /locus_tag="PC471_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003415975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HDF2788010.1"
                     /translation="MSTNHGANLYSLSSKYGFSKEEFMDFSSNINPFGTSSLAKQYIV
                     NNIDMVSMYPDPDYIDLKTSISNYCKCSIDNIVLGSGATELISSFIHTINPKQALLLS
                     PAYSEYEKELSKINCSIEKYFAKEEDNFHINLENLIKTINAKDYDLVVICNPSNPTGF
                     AFTKVEVREILKNTDSFLMIDETYVEFTDTDTYSCTQLVDDYSNLFVIRGTSKFFSTP
                     GIRLGYGLISNTNVKNEINKNLDLWNINIIASKMGEIMFSDLDFISNTISLMNTERDY
                     LLKELKNIKSLDIYNTKGNFILCKIKTKELTAKSLREQLLPQKIIIRDCCSFEGLDEY
                     FFRVCILKPNENKLLISSLKAIFK"
     gene            complement(24800..26008)
                     /locus_tag="PC471_000871"
     CDS             complement(24800..26008)
                     /locus_tag="PC471_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HDF2788011.1"
                     /translation="MKKKIGLVPKLIIGIIVGILIGSYAPEIIVQILVTVSTLFSAFL
                     KFVIPFIIIGFVTAGIADLATGAGKLLGITTGIAYGSTLIAGLLSFAVSTLIFPNFID
                     ASVASQIGDPEAGMLAPIFTIPLEPMVDVTAAIVFAFVMGLGISALRNHGKGETLFNF
                     FQEFQGIVTKTLSTVIIPLLPLYIAGTFANITIAGEVWTILGVFWKVYLVVIPLHFVY
                     MACQFTAAGVFSGKNPVRMLKNQVPGYLTAIGTQSSAATIPVNVGCAEKNGVSKQIRE
                     FVVPLCATIHLSGSMISITGFTTAVLMMNGMPHGIGVLFPYIAMLGIAMVAAPGAPGG
                     AIMSALPFLPMVGIPSDGGLASLMIALYLTQDSFGTACNVSGDNAIAVVVDKINDGMS
                     KKGNKEEQSA"
     gene            complement(26169..26558)
                     /locus_tag="PC471_000872"
     CDS             complement(26169..26558)
                     /locus_tag="PC471_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1232 domain-containing protein"
                     /protein_id="HDF2788012.1"
                     /translation="MEFLKIIINIVLDILKKILVRFKNAKFGLVFVFDLLKLPDFMTD
                     KRINIVDKIKVISVLIFTISYFVSGVDIIPEMIAGAFGFIDDAIVLIWSIGIVNEEIN
                     KYRVIAKKDKHSNIIENVEFSIKDEEE"
     gene            complement(26662..27663)
                     /locus_tag="PC471_000873"
     CDS             complement(26662..27663)
                     /locus_tag="PC471_000873"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HDF2788013.1"
                     /translation="MSTIIPKDYKSSLNVIDTQIAIKKLKDFFEMRLSNELNLIRVSS
                     PLFVLPETGANDNLNGEKAVSFDIPFMNKNAEIVQSLAKWKRLALKKYGFEVGSGLYT
                     DMNAIRKNEELDNIHSLYVDQWDWELVIDKSSRNEKTLKDVVKRLYGVFKDTEIFVCS
                     MYEGIKEILPEEITFITSQELEDMYPELSPKEREDKIVKEKKAVFITQIGKTLISGEK
                     HDGRSPDYDDWELNGDILFYNPVLDSALELSSMGIRVDEESLDRQLKLAGCEERKEFD
                     YHKMLLNGELPYTIGGGIGQSRICMYFLQKAHIGEVQVGVWPQDMIQNCSSAGIELL"
     misc_binding    complement(27722..27977)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(28111..29301)
                     /locus_tag="PC471_000874"
     CDS             complement(28111..29301)
                     /locus_tag="PC471_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HDF2788014.1"
                     /translation="MSKYNFDRVIDRVGTDCVKWDFRTNCSTKAQKDGLPFWIADMDF
                     ECAEPIIEALRKKVEHKIFGYSSNDSDKYFNAVCDWYKRRFNWEISRKDIIFSPGVVP
                     AVAILVRILTNSNEGVIVQKPVYYPFEAKIKSNNRDVVNNPLIYENGTYRMDYDDLEE
                     KAKCSNNKVLILCSPHNPVGRVWREDELKKVVEICKKYDLWIIADEIHSDLIRKGFKH
                     TPLQSLCPEYKDKIVTCIAPSKTFNLAGMQLSNIIVNNDELKKKYQEEVTAVGVATSP
                     NPFAIVATIAAYNESEDWLNELNDYLDNNIQFIDEYLKENLPKVKLVYPEGTYLAWLD
                     FTAYGLNEVELEDLMFKKANVLFDEGYIFGKEGIGFERINVACPQSLLKECMDRLKTI
                     FENL"
     gene            complement(29582..30703)
                     /locus_tag="PC471_000875"
     CDS             complement(29582..30703)
                     /locus_tag="PC471_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 peptidase aminoacylase family protein"
                     /protein_id="HDF2788015.1"
                     /translation="MGTNKDYIEYVHKVYDYLHTIPEKGFEEYKTSEFVLKELEKMGF
                     ENINKGVAGTGIIVTIDSKKEGPVFAIRADMDALEFIIDGEKKMIHACGHDGHTSMLL
                     TAAKLIKERNLVKRGKLKILFQPGEEKLYGALRVIDSGLLDDVEEMVGMHLRPGQDCR
                     LGKAIPAMYHCASCIMEFTIKGITCHGSKPHLGVNAVDAVCLAVNAINAIRVNPAIPN
                     SIKVTNISVKGETYNNIPDEAFMALDIRAESNDIIEELKEKAIHAVKTVVSSINAEAV
                     LISKDGVPAAEFDEELVNLAEETIKDVLGADASIGIFKNSGGEDFHYYTQKLKCKTTY
                     IGLGADATPGFHNPNVTFDTKALEYGVDIWCRLVEKRLG"
     gene            complement(30764..31249)
                     /locus_tag="PC471_000876"
     CDS             complement(30764..31249)
                     /locus_tag="PC471_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiG family protein"
                     /protein_id="HDF2788016.1"
                     /translation="MSEVAKKEVKVKKGNVIDAFIGGARNGFQISTNSMAPNVIFGFA
                     IISVFNLTGLLDVIGTIFTPVMSIFGLPGVAATAIMTIFISMGGASGVIAGLFTAGQI
                     SQAHVAILLPMLLLSGAQIQFMGRLLGTAELKTKYYPHMFVIATLNGCIAMLIMNYFV
                     V"
     gene            complement(31251..31919)
                     /locus_tag="PC471_000877"
     CDS             complement(31251..31919)
                     /locus_tag="PC471_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2788017.1"
                     /translation="MSKLESTTEKVEVKWYGYVALILGALFFSGIFKDAPGPLKVLDF
                     NNVLGSFGTLGTINDGVGTLAANFRGDGGTGPRDGWLYALTLIPSVMFALGVVRVIDH
                     LDGMKAAQKLLSPLLKPLLGLPGFAGLTLIASLQSTDAAASMTKELKDDGYIDEKQKA
                     VFCAFQFSGASAITNFFASGAALFPFIGDVPIFIPLALILIMKFVGANLLRLYLNKFD
                     KGEA"
     gene            32309..34333
                     /locus_tag="PC471_000878"
     CDS             32309..34333
                     /locus_tag="PC471_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009899983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDF2788018.1"
                     /translation="MDKKKLILITYDKLNSDHYKEELTNFFGDEIIIETQNILDGIKE
                     NLEGDVVLSLSPLTSNFLIKHFKEDIEIIHGTKALSKLGYEKMMKLPPGTKSLLMTTN
                     KTSAFEMATYLYKIGINHIDFVPTYPDCDEIYDLDTAITPGQIRFIPKYIKNIVDLGW
                     RKISLDTYMSLLVVLKLKNEKFIEKLYKLSKETLSHDFLNTSLDNISKLKTILYMTID
                     EIGDGLIFFNTFNKVTFVNKSLLNMLELDEKLIKSPSLMEYMPKSFLDKITKNLNIDN
                     MIIYIDEIDKKFILSKKPFYLYKNIEGCLITLKDVNNIEILEQKIRSDSVKRGYVAKY
                     KFNNIIGNSSIIKDCIKKAKKMALTDNPILITGETGTGKEAFTQSIHNHSNRKNKPFV
                     AINCASLPSELLESELFGYEDGSFTGAKKGGKKGLFELAHTGTIFLDEIGDMPHDLQV
                     KLLRVLQEKEIRKIGGTSIIPIDVRILAATNKDLEKLIIENKFRMDLFYRISMFTLDL
                     PPLRKRLEDIPLLLESFLKELPYKNIKLDKSLLEALNSYTWMGNIRELRNCVEYMAYM
                     GSNYLTINDLPQNISSKLNNNHMSSNMSIFNDLNQYDKNICISILKSLHMKPMGRTKL
                     MKFMEYNVTEYEVRNMLEYLTRNGYLISSKGRKGSSITEKGIKIIENNII"
     gene            complement(34563..35750)
                     /locus_tag="PC471_000879"
     CDS             complement(34563..35750)
                     /locus_tag="PC471_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3798 domain-containing protein"
                     /protein_id="HDF2788019.1"
                     /translation="MLRKITAFMLSICLIIGLTGCSLLVGKKDKEKIIDDFKVAVVTQ
                     PLSENKVQYNMVEEMAKEYEEENKIDKDKDGQTKVKQTIKHVVLPENFTSNIDSAINK
                     IVKLADDKEVQAIVVSTDQAGLLPALQKVKEKRPEIITISAPMGDDKNQLSQFVDVNL
                     GVSAEERGKVLAERSKEMGAKAFIHYASTDDLKDVNIAKRLEMIKETCKNIGLPFVQV
                     NTPNINTEEDKNKVKQFLNEDIEKQVKKYGKDINVFGVNEYMDEVILTKALELKYIVA
                     EQSNPSPIQTYPSVMGLKISEKDAQNYDKINDMISEKAKAFGMSNRLGGYPMPMDAFL
                     PSVAIYLATEMVKQDLTQEDVCDPDYLEAFTELRFGIGSEFTPLTEVLYNYQSVILSQ
                     LIY"
     gene            complement(35952..37223)
                     /gene="brnQ"
                     /locus_tag="PC471_000880"
     CDS             complement(35952..37223)
                     /gene="brnQ"
                     /locus_tag="PC471_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HDF2788020.1"
                     /translation="MRDIFIVGFALFSMFFGAGNLMFPPFLGMESGKDWLIPLIGFVV
                     ADAGIAMLVIIATARCKGSMDDVLRRAGKGLARVMSVAALACLALLVIPRTCATTYEM
                     GIMPIFGDHGPVAKAVFSIIFFGLTLAFTIRSSKVIDIIGKYLTPALLLVLFVLIVKG
                     IISPAGPMSPEHMIDKNLFGEGISQGYQTMDALGAASMATIMLMSIIAKGYTSDKDQI
                     GMTIKAGFVACVFLAVVYGGLTYLGATVSTLYDTSIPQASLLVEITSILLYGQVGKVI
                     LGVIVALACLTTSTGLTASISTYFEGISKASYKQLVIGICVASMLISNLGVDSIIAIS
                     VPILQTIYPVLLAIVVMELAGKHIKNDNAFKGAAYVTLVISLLSAINGMTGAVPFIQS
                     LPLAGLGFNWVIPAILGGIVGNFIKSNKQVA"
     gene            complement(37364..38017)
                     /locus_tag="PC471_000881"
     CDS             complement(37364..38017)
                     /locus_tag="PC471_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HDF2788021.1"
                     /translation="MTKIIKVQKPKFTGELEIIENLEDILEDIFNDEKIFNKIIENEV
                     ISDLDSVRVSFESCIFRNVSFENCNLKKIDLLDVVFENCNLSNTFFDGGSIYRVEFKN
                     SKLIGVRFDDCILKNVLFKDVLGKYSNFSFSKFKIVSILDSNFENSVFQEVKGDNLVF
                     EYTNLKKANFNGTKLSGIDFTTNNIEGIEIGIDDIRGAIFDVSQAIDLTKLMGIIIK"
     regulatory      38346..38447
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            38520..39863
                     /locus_tag="PC471_000882"
     CDS             38520..39863
                     /locus_tag="PC471_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HDF2788022.1"
                     /translation="MVPNTNNVQPGGNNSSLSFLDKMFKFSERGSSLKTEVIGGVTTF
                     LTMAYIVFVNPAILSEAGMDKAALITVTILATAIGTLIFAILGNAPFALAPGMGLNAF
                     FTYSLVIGAGIPWQTGLGVVFLSGCVFFTLAVTGLRKTIADAIPRELAVASSAGIGLF
                     LAFVGLKNMGVIIANPDTIVSLADFTPTVLIALFALILMAILEYKKVKGGILISIVVA
                     TIISVIFGFVELPTAIISTPPSIAPVAFKLDIVGALKFSLIGPIFSFMFIDMFDSLAF
                     LITCCKQMGLEDENGEIKDLGRMLYADVSSTLIGSFLGTSTVTTFGESASGIAAGART
                     GLASIVTAALFLATLIFTPLLGIVPTYAAAPALVMVGVFMFESIKQIDFADSKIAVPA
                     FVTVLLMPLTYSISIGLCFGFISYIIMHVVAKETDKISITLWIIGLLAVVNLVLS"
     gene            40042..41406
                     /locus_tag="PC471_000883"
     CDS             40042..41406
                     /locus_tag="PC471_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HDF2788023.1"
                     /translation="MSQLLIKNAYLITMNADREVYENGSILIENNIIKEVGKFDINIV
                     DKDAEIYDAKGKILMPGLVNTHVHLSQQLGRGLADDVVLLTWLRERIWPYESSFNYED
                     SLISSTACCVELIKSGVTTFLESGGQYVDAMVEAVDKTGLRACLAKSVMDTGDGLPEA
                     WQKTTDEEINTQLDLFHKFNNTSNERIKIWFALRTIFNTTEELLVRTKELADKYNTGI
                     HMHIAEIADEISFVKQNNGVGTVEYLDKLGVLGPNLLAAHTVWLTHKEIDLFRLHDVK
                     VSHDPGSAMKVVLGFASIPEMLEKGIPVSIGTDGAPSNNRMDLMRDMYLTSLIHKGRT
                     LNPTVVPAEEVLEMATINGAKCALLEKEIGSLEVGKKADMIILNPDTIHCLPMHNPIG
                     NIVYSMTSENVDSTICDGKWLMKERKVLVVDESELLNKVKKQASKIKDKAEVKIPSKF
                     KIIK"
     gene            complement(41584..43449)
                     /locus_tag="PC471_000884"
     CDS             complement(41584..43449)
                     /locus_tag="PC471_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIRAN domain-containing protein"
                     /protein_id="HDF2788024.1"
                     /translation="MENVFKRLQEFNGYDGYKESFEMNYLCIYESIPLREQVELANNL
                     VDEILNMYKSESNEIYLLEGSNSKSLICYFEIFMKKINTLVKEMIIDEKWLYKLTKEL
                     IYKSKKVEYVKLGLVLSEKYLNVENLREVVDTFSKSGEYVFYLSNTIKKLEFYNTYLF
                     NLSKKATGSIKVFAIVNMENLDSKINSYLIEDGYKDTKYERLLMNYIISIVDLNEYLE
                     KRDLDKEKINNLARLICNYLLSVEFKYIGNKLELVNRFLPTVVNYGTNFESLYSIFLI
                     AINVLKDENIECNKIEFEKEINGILLSEKWKNIYFEALRDASGKTEDIIKMSEIYDVN
                     LSFDDLLPYLNRDIRDFEVYWHISKKGTTSSRLKLLNFFEETFKIDDLIGKMKDIEKD
                     KLTQEYYDDMLFFIVLKGSKSLYPEGKNISLKGIFGNINEVRKESINILKRYREKLSL
                     EELKIVKEAYEKEKNVILKDELRRVLYESNNLKKEFVNIEKIKVDEHGKDIYLTSIAV
                     AGSRFRNREYLEKELEKSKIYYLTREKDNLYDEKAIKIVGETGYVIGYVPRKENYILS
                     NLLDGGKLLYCRVTEYNLYEDCIYANVYLSYKDVIETVENSLKMVLDKSRIKLIN"
     gene            complement(43685..44902)
                     /locus_tag="PC471_000885"
     CDS             complement(43685..44902)
                     /locus_tag="PC471_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDF2788025.1"
                     /translation="MNNKKYYPTAIALYFSYFLLGIGISILGQYKPEFSSMWGAKTLS
                     DGTLDVSIVLAVIAALGLGRLISYPFAGPISDKYGRKVSGLIGNFLHAIFFVGIVFSP
                     NFYIAYVFAIIGGAANSFLDTCVTPSCMEIFASLGTIANMFTKFTIALAQFLLPFIIG
                     FVAANSISFKVIFIVTAILIVVDAFLIAVLPFPPANNVIDNKGKTVKSEKMKFTPTSI
                     ALVCIGFTCTSTFVLWLNCNQELGKLYGMADPTKIQSFYSMGVICAVLITSLLIKKYI
                     KPIRILVIYPIIALLMLLVVYFVQTPTICMLGGFVIGYSAAGGVLQLTTSTANEMFPT
                     NKGKITSIVMIASSIANYVILNIAGIITKSGGVNGPKYVVLFNVAITFVGILLALFVN
                     MRYEKEKVYDYDV"
     gene            complement(45102..45965)
                     /gene="aroE"
                     /locus_tag="PC471_000886"
     CDS             complement(45102..45965)
                     /gene="aroE"
                     /locus_tag="PC471_000886"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HDF2788026.1"
                     /translation="MEISGRTGLFALIGTPVGHSKSPVMYNYSFKKLDLDYRYLAFDI
                     TVDKVKEALLAIKTFNIKGANVTMPCKSAVTEYMDELSPAARIIGACNTIVNDNGKLV
                     GHITDGVGYVRNLKENGVEVKGKKITIMGAGGAATAIQVQCALDGAREISIFNPKDDF
                     YKRAEQTVENIKKDVPECVVNLYDLEDTNKLYEEIQSSDILTNATLIGMKPYDNETNI
                     KDTSVLRKDLVVTDVVYNPKKTKMIEDAEANGCKAIGGLGMLLYQGAEAFNLYTGLEM
                     PVEEVNELCFK"
     gene            complement(46047..47969)
                     /locus_tag="PC471_000887"
     CDS             complement(46047..47969)
                     /locus_tag="PC471_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HDF2788027.1"
                     /translation="MKISSMFTPIRIGSMTVPNRFVVPPMGNNFANTDGTLSETSKAY
                     YLERALGGFGLITIESSVVDKKAKGGPRKPCLYDDSTIDSFKNVIDACHDAGSKVSIQ
                     LQHAGSEGNEKVAGHPLKAASAIPASNGRNTPLAITTEEIYELIESYGDAALRAQKAG
                     ADAVEVHCAHGYLVSSFISQRTNKRVDEFGGCFENRMRLPRLIIENIRKKVGNSLAIL
                     CRINSTDDVPGGIDVHDSSVIAAYLEDCGIDGLHVSRSIHIHDEYMWAPTTLHAGFSA
                     ELVTEIKKAVSIPVITVGRYTEPQFAELMVRQGRCDLVAFGRQSLADPEMPNKAKNGK
                     LDEMIPCIACLQGCVPNMFQGKPIACLANPILGHEAELKPAEISKEVLVVGGGVGGML
                     AAWVCAKRGHNVTLVEKSEVLGGQMRLAAYPPGKGDITNLVRSYISKCNQYGVKICTN
                     TEATVELIKEKSPDVVIIATGATPLVLPIPGINDSGLIHAVDLLDGKKSCGKKVLVVG
                     GGMVGCEVAAFLGEQEHEVTVIELREEVGADVISEHRKFLMNDFENYNIHTITGAKVS
                     KFYDDGVDYLLTDGTEHDLKGFDSVVLAMGSRNYDPLSEVIKEVVKETYIVGDAVKAR
                     RALDATKEAFEVALNI"
     gene            complement(48033..48881)
                     /locus_tag="PC471_000888"
     CDS             complement(48033..48881)
                     /locus_tag="PC471_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HDF2788028.1"
                     /translation="MNNQKNLNIPITISSYTLGTEVSFRDRVRIAKEAGFDGIGLRAE
                     NYIDAKNAGVTDEEMIAILNEYDMKVTEVEYITQWGREEDRTEAQREKEQNVYHMAHL
                     FNVKHINCGLLEMIPEEQIITALGELCDRAGELIIGLEFMPYSGVPDLATAWRVVKGC
                     NRENAMLILDTWHWARAKQTAEDLAPVPADKIVSIQVCDVLETPYEKLRDESLHDRLA
                     PGEGYGNTEEFVKIIKEHNISPRVIGAEVISDSLISKGLSTAAETVFNATKKVLDKAW
                     SEVSPK"
BASE COUNT        16875 a         8081 c         6151 g        18004 t
ORIGIN      
        1 taatgattac tattatacaa taaaaaatat gtgttaaata ttattttatg gaggttttat
       61 aagaatatga aatttaaaaa attggcatca ttaatcttag tttcatcatt aatgcttaca
      121 tttacagcct gtgcttccaa tgataaagac aagtctagtg gaagcaaatc tggtggaact
      181 gttgtaatac ctatggcaag tgacccagat ataatcaatg gagcttttgc aaacgttaaa
      241 gaagactctc ttgcatcaaa tatagtttat gctcctcttt atacttatga aaaaggaaat
      301 ttagtaaatt atttagcaga aaaagtagat tttaaagata gtaaggaatt aactataaaa
      361 ttaaaatcca atttaaaatg gcatgatgga aagccaataa ctgctgaaga tgtcttattt
      421 acatttaata cagttttaga cgaaaaacaa aattcaccat caagacaata cttattggtg
      481 ggtgagaaac cagttaaagt agaaaaaatt gatgatttaa cagttaagat tactttacca
      541 actgcaagtg aatctttctt atatggtata tctaaaattt ctccaatacc aaaacatgtg
      601 tttgaaggag aatcaaatat agctaagtca gaaaaaaata ataatccagt tggttctggt
      661 gcattcaagt ttaaggaatg gaaaaaaggt gaaagcatag tatttgaaaa gaatgcagat
      721 tattttggtg gagaatctaa ggcagattct atagcgctaa aaataattcc taatgaagca
      781 tctcaagaag cagctttaaa taatggcgaa atttcactta tgaagacatc tgctgaagga
      841 tatgaaaagg caaaatcaaa ctcaaattta caaacttata cttatagtga agaaagatta
      901 aactacattg tatttaacca aaatatatca aacatggcta acaaagaagt aagacaagcc
      961 ttatcatatg ccttaaatag aaatgaaatg atagaatcag catatggaaa agaaggttct
     1021 gtacctgcta aatctatatt agttccagaa gcagacttct atacagaaga aggtgttgaa
     1081 ggatatgacc aagatacaaa taaggctaaa gacttattgg ataaaagtgg tgttaaaatt
     1141 gataaattaa aaataggtta taacacaggt agatttgggc ataaaaacta tgccttagta
     1201 gctcaacaag agttgaaaaa aattggtata gaagctgaaa tagttcctta tgaaagtaag
     1261 gcattcttca atatactatt tagtaatagc actgaatgtg atatgtatgt aaatggttat
     1321 gcttggggat tagaaccaaa cccatataga ggtatgtttg aaactggtca atattgcaac
     1381 caaactaaat attctaatgc tgaaatagat gctttatggg aaaaaggatt tactgagtta
     1441 aacaaggaaa aacgtgaaga aatatataag caaatacaac aggatatatc taaggatgcg
     1501 cctatttaca ctatagatta tgaacaaaat ttaatggcag ctcaaaaaaa tctaaaagga
     1561 ataaaagatg ctaaaccatc tcctgcaata ttatttgaag attggtctaa actgtatgta
     1621 gaataattga tgtaaaattt tatattatta attagaaaag gagatgtcta aaaataatat
     1681 tgttttatta ttttaaaacg agacatctct tttaattagg agaatttact tatgttaaaa
     1741 ttaatattga atagagtaaa agttttaata cctatgttaa tcttaatatc tatattatca
     1801 tttggacttt tagaattagc tcctggtgac ccagcagatg catatataaa tcctttaatg
     1861 tcttcacagg atattgaaaa tataagggtt aatatggggc ttgataaacc agcatatata
     1921 agatatttaa actggttgaa aaacacttta agtggaaact taggtatttc ttatataaac
     1981 catatgcccg ttacagaaca aattatggaa aaaatgggaa atacatttat tcttatgggt
     2041 acttctttga tattttcaat attggtagcc atacctcttg gaatattttt agctgtaaat
     2101 aaaaattcaa taactagtaa agtatcatct gtttttaatt atataggtgt ttctatacct
     2161 tctttttgga taggtatgat actcatttca attttttcag ttaaacttaa cattttccca
     2221 tcaggaggta tgcatactat tggaaatgat agtattggag atttagcaaa acatttagtt
     2281 ttgcctgtaa taactttagg tctatataat actgctattt ttacaaatta tgtagaagct
     2341 agtgtaaatg aacaattaaa aaaacaatat gttattacag caagagcaaa aggattatca
     2401 gaaaaagtca tactatttaa acatgtcttg aaaaattcac tgacatcatt ggttacaatc
     2461 ctaggcatga gtatacaaaa attagtcact ggtgcatttg ttacagaagt agttttctct
     2521 tggccaggta tgggaagatt aatgattgat tcaatttttt caagagatta tactgtaatt
     2581 atggcaataa ctatgttatc agcactattt ttaattttag gtaatttagt tgctgatata
     2641 ctatacttat taattgaccc taaaataaaa tctagcaaag gaggattcta gttgtgttta
     2701 caagtatctt aaaaaaatta aaaaaagata aatcctttgc ttttgcatgt attactctta
     2761 gcataataat catattaatt atagcttctt ttatattaaa tatagactct aatgcaattg
     2821 atactacttc taaacttcaa gaacctagct taaagcatat ctttggtaca gacgaacttg
     2881 gtagagatta cttctctaga gctctttatg gtggaaggat ttcacttagt gttggtattt
     2941 tatcaatgct tatttctgta gtatttggta ctgctgtagg tgttattagt ggatatattg
     3001 gaggtaagct agaccaattc ttgatgagat tagtggatat gcttatgagt ataccaagtt
     3061 tcttagtttt gattgtatta aatacttatc taacacctaa agtttccact attatactaa
     3121 ttattggatt cttaagttgg atggaagttt caagaattgt acgtggcgaa acattaaaaa
     3181 taaaggaaaa tgaatactgt ctagctgcta aagctactgg tatcaaaact agaaatatac
     3241 tattaagaca tatagtttta aacttaaaag agactattgt agttgctggt tcgttaaata
     3301 ttgctaatgc tatcttaact gaatctgctc ttagtttttt aggtcttgga atccaattgc
     3361 ctcttgcatc atggggtaat atgcttcaag atgctcaaag acatattttt gacaagttat
     3421 atttagctgt atttccagga ttacttatat ttttaacagt catgagtttg actataataa
     3481 gcaaaaaatt ttctagtaat acttaacaaa ttttatatag aaaataaaaa cagtgattat
     3541 ctaaagatat acactgtttt tatttttcgt ttaaaaaaat acatattttg tatattataa
     3601 ctttaaatac tactctacca tgttatgcaa agcgtattat tgctctcaaa catatagatg
     3661 aaaaactaat agtttattat aataaaaatt gatttattta tttgttattt taataacatt
     3721 tttaagagtt ctttttacta aagaaatcta taataaattt aaatacatgt tggtctacat
     3781 ggctataacg ttctttttta ggtctacata atataacttt ccatgtaata ggatgattaa
     3841 aaggcttttc tataattttg ctattttcaa aaaaatcatg aactggtgat ggtaaaattg
     3901 ttataaaatc agaatttgtg gttgatagca acaaaaaatc ccatgaacca gacattatat
     3961 atctttttaa cttaacattt tcatcagtaa atttttgcat tagcttatga tgtatcataa
     4021 aagtaggatt aaatattgca agtaactgct cattcaaatc gttccaatct atcttatcct
     4081 tcttagcaag tggatgattt atatttaaaa atgctgtaag ttcatcttct tgaagcacat
     4141 gttcagttac tacactttta tcaattttat gtggatgtaa caatactgca taatctaatt
     4201 cctgtaagat aagcattcta tttaagtcat atgcaccctt ctctactata tcaaattcta
     4261 tatctggatt atttgcaact aactgtgcaa ctacatctga aaatgctatt ccaagtataa
     4321 gtggaggtat tcctattcta acttttcctt taaattgaac tgaatcttct cttaaatctt
     4381 ccatcatatt tttgtattca tttatgatat gttctgcatt tacataaaat ctttctcctg
     4441 ctggtgttaa cccatttaac ctgcctttat acctttcaaa taaatataca tcttcacttt
     4501 cttcaaaatt ttttataatt tgacttaatg ctggttgaga tatgtgaagt gtcttagatg
     4561 ctattgataa attaaaacca gatttcacta tttcaacaaa atatttcaac tgttttatat
     4621 ccattttaaa cccccttccc caaaagtata atttagatta tattttttct tctaatgata
     4681 ttttatagga aaatcaacat aatttcatta atattttaac aaaatatttt tctaaatcta
     4741 ctataaattt ttatcctata aatttttctt atacactata taaaaaatta gacttagaca
     4801 aaacgctttt ttcgccgtaa tctataatta gaaacaggaa aacctaaagc attaggaata
     4861 aaagtaattg aaatacttaa aaacaagctt gtattaaaaa agtactttat tatagtgttc
     4921 cctaatttct ttattcagaa aatttttaat tatcattcaa gaaaggtaac tattagatat
     4981 gaaagatgta gtaatcgtat cagcagtcag aacacctata ggaagttttg gaggtgtatt
     5041 taaaaatact tcagctgttc aacttggaac tatcgctgtt aaagaagcga tatcacgtgt
     5101 aagacttaat ttatctgaaa tagatgaagt aataattgga aatgttcttc aaacagggct
     5161 tggacagaat gttgctagac aaattgctat aaatgcaggt ataccaaatt ctgttccaag
     5221 ctacacagta aataaattat gtggttcagg gcttaaaagt gtccagctag ctgcccaatc
     5281 aatcacatct ggtgaaaatg atgtggtaat tgcaggtggt actgaaaata tgagccaagc
     5341 tccttatatc gttccaactg ctcgctttgg tagtaaaatg ggtgacataa ccatggtaga
     5401 tagtatgcta acagatggcc ttattgatgc tttcaatcag tatcatatgg gaataactgc
     5461 agaaaatatt gcaactaaat ttgaatttac tagagaaatg caagataaat tggcattaga
     5521 aagtcaaaat aaggctgaga atgctataaa aaacaaccgc tttaaagaag aaatcgttcc
     5581 agtagatgtt ttaatacgtc gaggaaaaat agaaactatt gataaagatg aatatcctaa
     5641 attaggtatg acatttgaag gtttatctaa acttaaacct gcattcaaaa aagatggtac
     5701 tgtaacagct ggaaatgcct caggtattaa tgatggtgcg gctatgttaa tactaatgag
     5761 ccaacaaaaa gcagacgaac taggtattag accacttgct aaaataaaat cctatgcttc
     5821 agctggtgtt gaacctgaag tcatgggtac tggtccaatc cctgcaacta gaaaagcact
     5881 gaaaaaagct ggacttagca tcaatgatat agacttaata gaagcaaatg aagcgtttgc
     5941 tgcccaagca ttagctgtta aaaatgaact tcaaatagat tcatcaaagt taaatgttaa
     6001 tggtggtgca atagcattag gacatccaat aggagcaagt ggagctagaa tacttgttac
     6061 cttaatttat gaaatgcaaa aacgtaaagt tgaaactgga cttgcaacac tttgtattgg
     6121 tggtggtcaa ggaatatcaa tggtggtttc acgataggag gaaatatgaa taaaatagtt
     6181 agcattgatg aagctctatc tcatgtaaaa gatggagata caataatggt tggtggattc
     6241 atggccaatg gttcaccaga aaatttaatt gattatttat gtagtaaaaa tataaaagat
     6301 ttgacactaa tttgcaatga tactggcttt attgacaaag gagttggcaa aatggttgta
     6361 aataagcaat ttaaaaagat aattgcttct catgtaggat taaataaaga aactggaaga
     6421 caaatgaata acaatgaaac agatgttgag ttagttcctc agggaactct tgctgaacaa
     6481 atacgtgcag ctggttatgg tctaggaggt atactcacac cgactggtat tggtactctt
     6541 gttgaggagg gaaagcaaaa aattgttgtc gatagcaaag aataccttct tgaaaaacca
     6601 atttatgcag atgttgctct tctatttgca tcaaaggtcg ataaagcagg taatctagtt
     6661 tataaaggct ctatgaacaa ttttaataat ttaatggcat ctgctgccaa aattacaata
     6721 gttgaagcag atgaaatcgt tgaaattggt gatattgacc caaatgaagt taatacacca
     6781 ggtatttttg tgaattacat cgttgaagga ggtaatttat aatggataaa ttagaaatgc
     6841 aagaatatat tgcaaataga gtatctaaag aattaaaaga cggagccgtt gtaaaccttg
     6901 gaataggtct accaaccaaa gtagcaaatt atattccgga taatgtaaac gttatcttac
     6961 aatctgaaaa tggcttttta ggtttaggtt cagccgaaga tggaaattca agtgatgaaa
     7021 ctattgtaaa tgcaggtggt caaccagtta ctatacttcc tggaggatgt ttctttgata
     7081 gtgctacttc ttttggaata atcagaggag gtcatgttga tataactgtt cttggcgcac
     7141 ttcaggttga caaatatggc aatatagcaa actatatgat tccaggtaag atggttccag
     7201 gtatgggtgg agctatggac ctagtaactg gagctaaaaa agttattgta gcaatggaac
     7261 atacttcaaa aggttctgca aaaatattaa ataattgtac tcttccactt actgcaacaa
     7321 atgctgtaga tttgatagtt actgaaatgg gagttatgga agtaacatca gatggaattt
     7381 tacttaaaga aataaatcca gctttcacat tagatgatgt tatttctgct actgaagctc
     7441 ctttaatatt gtcagattca ttaaatggaa aaatatctgt tgtttaaaat aattaaaact
     7501 agaaaataaa aatctcttag acgtattaca tataaatttg tattgcttaa aatattaata
     7561 taaaaataaa ataaaattgg aggaaatgaa aatggttaaa gataaagttg tattcgtaac
     7621 aggagctgca agtggaatag gtaaacaaat agctgaatcg ttcttaaaaa atggtgctaa
     7681 agtaatgttc tctgatataa atcaagaagc tttaaataat accataacag agttacaaaa
     7741 aaaaggttat gattgcatga gtgtaaagtg tgatgtaact aaggaagaag aaattaacca
     7801 cgctatagac aaaactgttg aaaagtatgg cagattagat atattaataa acaatgctgg
     7861 acttcaacat gtttctatga tagaagattt cccaactgat aaatttgaat ttatgataaa
     7921 aataatgctg acagcacctt ttatagctac taaaaaagca tttcctataa tgaaaaaaca
     7981 aggctttggt cgtataataa atatggcctc aatcaatggt gtaataggat ttgctggaaa
     8041 ggctgcttat aactcagcaa aacatggact tattggacta acaaaagttg ctgctcttga
     8101 agctgccgat tctggtataa cagttaatgc tttatgccct ggatatgtag atactccact
     8161 tgtcagaggt cagttcaatg acctttcaaa aactcgtaat ataccattgg aaaatgtctt
     8221 agaggaagtt ttatacccac ttgtaccaca aaaacgccta ttagatgttc aagaaattgc
     8281 cgactatgct atgttcttag caagtgataa tgctaagggt attacaggac aagcttgctt
     8341 actagatgga ggatatactg cacaatagtt attatattta gggcttgaag aaagtattct
     8401 tcaggtcctt ctactaagga gggatattta tgagtattat cgcgccaatt ggtataatta
     8461 ttggtatcat agctattata tatttttcag ttaaagaaat tcatataact atagctgctc
     8521 caatagcaac tttaatagtt gttattttaa ataaaatgga tgttgtaacg tcaatgctag
     8581 gtgctgacaa aaacaattat atgggtgcac ttggaaacta tataatgagt tattttgcaa
     8641 tattcttatt aggctcaata cttgctaaat ttatggaaga aagtggagct actgtatcaa
     8701 tagctgattt tattttaaat aaatttggct ctaatcatcc ttatagagtt ttagttgcta
     8761 tatttatagt aagttttata ttaacgtatg gtggtataag cttatttgta gttatgtttg
     8821 cggtcattcc acttgcaaga actctattta aaaaacttga tatttcttgg aaccttattc
     8881 agataccact ttggcttggt attgcaacaa ttactatgac tataattcct ggaacaccag
     8941 ccattcaaaa tgttatacct attcaatatc taaatacgtc tcttactgca gctactatac
     9001 caagtattgt aggtagtata ggttgtgcta catttggact tgtctatatg aaatatgctc
     9061 taaacaaaag tattagaaaa ggtgaaactt atgcaactta cacttcagaa ttagaagaag
     9121 aatctttaga tagagaatta ccaggatttt ttgcaagtat ttttcctcta ttagcactcg
     9181 taattatagc tataactgga agtacttttg gttcagaatt tatgaaaaaa aatgtaattt
     9241 acatagcttt aatagttgca attcttttat ctgctttttt attccataaa tttctatcat
     9301 ctaagataca gacattgagc ataggtgcta gtggttctat aggtcctata tttgctacat
     9361 cttctgcagt tgcatttgga gcagtaataa tggctgcacc tggttttgat gtatttagca
     9421 aacttataat gtcaatacca ggtagtccac ttataagtct tactgttttg acttcaacaa
     9481 tgtcagcaat aacagggtcc tcttctggtg cattaggaat agtaatgcca aactttgcag
     9541 attattattt aagtactggt ctaaatccag aattaattca ccgtgttgct acaatagctt
     9601 caaatatact aactatagtt ccacaaagtg gtgtttttct tacattctta agcttatcta
     9661 agttaacaca taaaactgga tttaaagaaa ctttcattgt agttgcagtt ggttcactaa
     9721 tagcagaaat tattgtaatt acattaggat tactaatgta ttaatataaa acaagttaaa
     9781 attcataata tgaaaggtgg tgtctcaaag tgaacttttt acttaatgag acactctttt
     9841 tatatgaaat aacatatgcc tatcatttaa acaataaaaa aggccttatc tctattttga
     9901 gacagaccct ttttatttct taaagattta aataattata atttgttgct aatacgaaag
     9961 catctactga ataaatttaa ccaaataaaa aaaaatttat cataactata aatttacgtt
    10021 atggaattat tataaaaata ttattttctc taactaagta attccatact atacaggctt
    10081 ttaatacaaa ttgtcaaaca cactgaaata tacctattta ttccatatat atctttttta
    10141 catatcttta cagtaaaaaa agtcttaatc ttatgttagt atagattcac gggtaacccc
    10201 ctacactaaa ggttacaaat cagtaacatt tttagaattt ttagattaag aggtgtttaa
    10261 tgatgagaag aaatatacca acaaaattaa aaactatatt agctttagga gtgttaactt
    10321 tagcaacagc ttcaagtgct gttactgcaa gtgcttcatc acttgaaaat aatgtaaata
    10381 taagtaacaa aagtttagta tatagtaata ctaaatacaa gttttctgca aaaccaaatt
    10441 gttctgtaaa accagaactt aagcctgata aagataactc ttgtaaggat aagaatcata
    10501 atgacaagaa ttgcaacaac actaataaac ctgaggacaa caataattca gactctacta
    10561 ataagcctga taacaataaa cctgagtcta ataaacctga agacaacaat aattcaggct
    10621 ctactaataa acctaataat aataaacctg agtctaataa acctgaagac aacaataatt
    10681 caggctctac tgataaacct gataacaata aacctgagtc taataaacct gaagacaaca
    10741 ataacaataa ttcagactct actaacaaac ctgatgataa taacaactca ggctctacaa
    10801 gtgaaagttt ttcagcttac caaaaagaag ttgttgactt agtaaatata gaaagagcta
    10861 aagctggatt aaatcctctt actcttgatt cttctatatc aaatgttgct acaaaaaaat
    10921 ctcaagatat gattgataac aactattttt cacacaattc tccaacttat ggttctccat
    10981 ttgacatgtt gaaaaaattt ggaataagct ataagacagc aggtgaaaat atagctatgg
    11041 gtcaaaaaac tcctaaagaa gttgtaaacg catggatgaa ttctgaaggt cacagaaaaa
    11101 atataatgaa tccaaatttc agtaaaatag gtgttggagt agcacaaaaa agtggtggtt
    11161 caatttattg gacacaaatc tttgtaggat aaaatactct ttttccatac atatcaaaag
    11221 agtatctcta agtgataaaa aacacaaatg agatactctt ttgtatctat tttaaaatta
    11281 tcaaatccaa atatacatac tataaatatt atttgttctc ttaattttag tgtgtagaac
    11341 aatatataaa aagtattggc aagatgaaaa tggaaaactt aaaaaagata tttaataggt
    11401 aaaaacaaaa tcttaatatc ttcataaaaa aaggctgtct caaaatagag ataaggcctt
    11461 tttcattgtt gaaatgatta aaatatattt gatttcatac aaaaagagtg cctcatgaac
    11521 taaaaagctc actttgagac actctctttt atcaatttat ataagcatta taatacttta
    11581 atattattca gctaattttt tgtatccttc atatctttct ttagcatcag cttcagcttt
    11641 agcaaataat ccatcagctt cttctgggaa ttgtttagct atagcagaat atcttacttg
    11701 tgcaagtatg aaatctttga agcttgcagt tggttctttt gaatctaaag tgaatgggtt
    11761 tttaccttca gcttttactt ctggattgaa tctgtataaa tgccagtatc cgcaagcaac
    11821 agcttgtgct tcgttagcta cagttcttcc cataccttct tttaatccat ggtttataca
    11881 tggagcataa gctattatta gagatggccc atcatgtttt tcagcttcta taacggcttt
    11941 gataaattgg tttttatcag cacccatagc aacttgagct acatatacgt ttccatatgt
    12001 catagccatc attcctaaat ctttctttct agatttttta cctgaagcag caaacttagc
    12061 catagcagca gctggagtag ctttagaagc ttgacctcct gtatttgaat atacttctgt
    12121 atcaaataca agtacgttta catcttcacc agatgcaaga acatggtcta atccaccgta
    12181 accgatatca tatgcccaac cgtctccacc tagtatccat tgagatctct taactagata
    12241 atcttttcta tcttttatag cttttactaa ctctttagct ttttcatctg tacaatcaca
    12301 agctggtttt tctaataatt caacaacctt agcacttgct actttagaag cttctccatc
    12361 atttctagaa tctaaccact ctgtaaatac tgctttagca tcttcacaga tacccataga
    12421 aactaactct gtcatagcat ctactatttt atttcttatt tgcttaacag ctgtgtacat
    12481 accaaatcca tattcagcat tatcctcgaa taaagagttt gcccaagatg gacctttacc
    12541 ttcatggtta caagtataag gaattgatgg tgcagaagct ccccatatag aagaacatcc
    12601 agtagcatta gctatcatca ttctatctcc atataattgt gtaactactt taatgtatgc
    12661 agtttctcca catccagcac atgctcctga aaactccatt aatggttgtc taaattgact
    12721 tcctttaaca gtgtttacag gcataacatc accttttgga gctacctcat caggatttac
    12781 tgcaaatgcc caattatcta actctgtatc aagttgagta tctattggct tcataactaa
    12841 agctttttct ttagctggac atatgtcagc acagtttcca catcctgtac agtcaagtgg
    12901 actaagttgc attctatatt ttaatccttc taatccttta cctaatgctt ttttagcttc
    12961 aaatccttct ggagcattag ctaattcttc ttcagtaact aatactggtc ttatacatga
    13021 atgaggacat ataaaagaac attggttaca ttgaatacag ttatctatta tccattcagg
    13081 aacatcaaca gctataccac gtttttcata agctgcagta ccacaaggga atgttccatc
    13141 ttctatacca ttgaatgtac ttactggtaa gttgtttcct tcttgtgcag tcattggtct
    13201 tagtatattt tttataaatt ctggttcttt aacttctcct ttatctgcat caacagcatt
    13261 ttcccaagaa gctggaactt caactttaac taaagcattt attcctgcat caacagcagc
    13321 atagttcatg ttaactattt tttcaccttt tttaccataa gctttaacta tagaatcttt
    13381 taagtattta acagcatctt cttctggtat tatttcagct aatttaaaga atgcagattg
    13441 cattatcata tttattctgt ttccaagacc tatttcttgt cctagtttaa cagcgttaac
    13501 tgtatagaat tttatatcat ttttagctat atattgcttc atatgagctg gtaaatgttc
    13561 ttcaagtcct gcttggtccc atatagtgtt aagtacaaat gtaccacctt ttttaagacc
    13621 ttttaataaa tcatattgat aaacatatga ttgtttatga caggcaatat aatcagcttc
    13681 atctattaaa taagtagctc ttattggagt atcaccaaat cttaagtgag acatagttat
    13741 accaccagat tttttagagt cataatcaaa gtaagcttga gcatattttt cagtatggtc
    13801 accaattatt ttgatagctt gtttattagc tccaacagta ccgtcagaac ctaatcccca
    13861 gaacttacat cttatagttc ctttagaagc tatttttatt ggctcagatg gagttaaaga
    13921 agtattagtt acatcatcaa ctatacctac tgtaaatcca tttttaggtt gttctgaaac
    13981 taagttatca aaaacagttt ttatatctga aggagtagta tcttttgaac ctaatccgta
    14041 tcttccacct attatcatag gagcattttc ttttccatag aatacatcac gtacatctaa
    14101 gtataatggt tcaccagttg aacctggctc tttagttcta tcaagtacac atattctttc
    14161 aacagtagat ggcatagcat ctaagaaatg tttcattgag aatggtctat ataaatgaac
    14221 tttaactaaa ccatattttt ctcccttagc atttaagtaa tctatagttt cttctattgt
    14281 ttctgttact gaacccatag ctattaatac gtatttagca tcttctgctc catagtaatc
    14341 aaataaacta tgttttctac ctgttaaatt actcattttt tccatgtatt tttcaactat
    14401 acctactata ttttgatagt atttattaga agcttctcta gtttggaaat atatatctgg
    14461 gttttgagca gtaccacgag caactggatg atttggagat aaagcattat ctctaaatgc
    14521 ttgaactgct tcaaagttta ataaacttgc ataatcttca ttttctagta attcaacttt
    14581 ttggatttca tgtgaagttc tgaatccatc aaagaaatgt ataaatggta atctaccttc
    14641 tattgcagct aaatgtgcaa caggtgctat atcagctact tcttgaactg aaccagaagc
    14701 taataataca catccagttt gtctagcagc cataacgtct tggtgatccc caaatattga
    14761 taacgcttga gatgctaaag cacgagcact tacatggaat actcctggta ataactctcc
    14821 tgcaacttta tacatgttag gtatcattaa taataaacct tgagatgctg taaaagtaga
    14881 agttaaagct cctccttgta aagaaccgtg gaatgttcct gatgcacctg cttctgactg
    14941 catttcaaca acattaactg tttgtccaaa tatatttttt cttccttgtg atgcccactc
    15001 atcaactact tcagccatag ttgaagatgg tgtgattgga tatatagctg ctacatctgt
    15061 aaacgcatag gcaacatgag ctgcagctgt atttccatca agtgttttca taaacttagc
    15121 cataatatgt atcctccttg atagctacaa ttgtacttgt ttataacaag tctatttttt
    15181 tatatttaca tataatttac acaacaaaac tataaacaca catatagtcg cgcaaatact
    15241 ctatcttcta atataatact acagattaga taaaattcct ttgaaaattg taaattaatt
    15301 acatatttaa gtagattttt tttaaaatta aatatgttaa gtttttttca taattttgta
    15361 tacaatatat aataaattaa gttagatttt tcaaactttt ttatctattt gttcatatta
    15421 gtataaaaag taaaatgctt atccttatta catgtaaatt tttatatata taattttacc
    15481 atattacatc atatttttgt aagtttttat tacatttttt ataatacccc tttattttat
    15541 aaattaatct aatcgattaa atttatccta tagaaattta ttacatttaa aaatatgcta
    15601 gcaaataaaa tatctactag cataaagtct atttataata ttaatcaact attaattcta
    15661 ctaaatctcc atttttaacg cctgctgcat ttccctcttc catatcaaca tgcatttcta
    15721 gattaaagct gtcactagct cttactaata cattttcaaa tactaatgct cttggtccag
    15781 atgttttaac ttttactatt tgcttatcag ttacaccaaa tttagcagca tcttcattgc
    15841 tcatatgtat atgtctagaa gcaactataa ctccttcttt catttcaact tctccagcag
    15901 gtcctactaa tttaacacct ggagttccag ctatatcacc agattctttt attggtgctt
    15961 ttactcctaa tgggaaacca tctgctaaag atatttcaac ttgagtatga gctctagctg
    16021 gtcctagtac tctaactcct tttaaagttc cttttggtcc tactaaatcc actttttctt
    16081 cacaggcata ttgccctggt tgagataatg gtttaaattt agttaactca tgacctgccc
    16141 caaataatac gtctatatca gcttgactca aatgaacatg tttattagat aatgctattg
    16201 gtaatttcat tgaaattccc tccttttaat tatttaaatt attgcaatac aattatacat
    16261 aatattacct acaaaatcaa attatattga atcaatatat atctttaaat ttttatatat
    16321 tatatcttta aattcttgat tatctatata agatacatca tctgtattgt ttaaatatat
    16381 attgtgtaaa agtttagtat ttatactctt aaagaaaaaa tccatcacaa tctgtccacc
    16441 tttaaactgt gaatcatatg atttactccc ccccactgca atatacattc ctatccgttt
    16501 cttacttcta tcaattgaag gtttatttaa tacatattta cttgcatata tagcttgagt
    16561 tctatcaaca acagtcttta atttagctga tattgaatca aaatgtacag gacttattac
    16621 tatagttcct tttgaatcat caaacatttt atataatggc ttcatatcat cattaaactt
    16681 gcaataccct gttttttcac aaaatccaca tgctgtgcaa tattcaatat ccatattgta
    16741 gatattaaat attttacaac ttaatccaca ctcttctagc atagctttta cttcatttgc
    16801 tataaaactg caatttttat taattcttgg acttccattt attattataa tctctggact
    16861 tttaagtcct tttttatcgt ttgaatccat aatcaatcta cctatccttt aacataacta
    16921 ttaaaatatc atcatcataa ttatcttttg atgagaagtt ctttaaatcc aattttagat
    16981 tttctactat taactgttga tttaatctaa aattttcttt tagaaagttt tctaatcggt
    17041 ttataccata ctcttctttt gatgaatttt ttatctctaa aattccatca gtgtacatac
    17101 ataccattgc ataatcttcc attgaaagag tattattttc ataagtagca tcttccatta
    17161 ttccaatagg tatgcccttt ttacaattta aattattttc tattgtgcca tctttcttta
    17221 caactacagg actatagtga cctgcattag acacagtaat catattttct ttagtatcta
    17281 taatacctat caaacaagtt gtaaatactc ccatcttgtc aaattcatca tataacatcc
    17341 tatttaaatt tgtcattatt tctcttggtg tattgtattg attgcataat actttaaaag
    17401 caccttttat cattgcaaca acataatttg ataccattcc atgtcccatt acatcagcaa
    17461 caatataaac tatatgttcg tcatccactt ttgtagcata ataaaaatct ccacctacta
    17521 cccttgctgg tttatggtaa aaataaagtt ctttctcatc attacaatcc attttacctt
    17581 tatccataat aagctgttgt tgtttattta atatgttcaa ctcgttttca agaactttgt
    17641 gttttacttc cttttcatta tgttcatata attgcatacc aacagaaact tgtttggaca
    17701 atatactaag aatatttaaa tcatcactag tatagttttt gctatttgca caaactatac
    17761 atcctatagt atcattttta tcctgaagtt tgtaatatat ataactttcc aaatttattc
    17821 ccttttttga gcatggtttt aaattattta gatacccccc caatgactct ttctttaaaa
    17881 acatatcaat cataggatat aaatttgttt tctttttttg ctcaacatta taacaactaa
    17941 ctatagcatc cttattatga tgactattta taattattaa agcttcttta ctattagtaa
    18001 tattacacgc ttgtttactt aaattttcca aaaagccatc aatactctta ttaaggtata
    18061 gttttttagt agcttcattt atggcactta ttgagcctct tagtttatca attaaaattt
    18121 gtacattatt atttttatta gttatttcta ttgataaact taagagtgat gcaacagatg
    18181 atataaaatc tatatcttgt tctgttatat aatcatcctc ttttaaaaaa caagtcataa
    18241 atccaacaac tacgttattt attataaaag gaaaaactat tctcccaata taaccttcag
    18301 cttttgctaa atctcgttca ccttctgctc tagaatcttc aaatacattt tccacatata
    18361 ttatcttttt ttcatttaca gcctcatgta tatactctgg atatgtgtca aaatcaattt
    18421 ttgcaccttt aagactattt attggaaaaa tttgaggtac atattctaag gtttgcgaac
    18481 atactaagta ggcatattta gaatcatttt tataaaatag attcacacat gcctttgtag
    18541 ggtgaacaac ttctaacatt ttttctatta tgtcatcttt tatattaaaa aaatctgtag
    18601 attcagtcac catagcggat atttccacta aattcttcat tttataactg ccattagtca
    18661 tactcaattt ctccccctat atattaaact atttattagt attttaacat acattcttta
    18721 tagtaaaaac aaaaacaacg acaaaactag caactaatat ccagttaaat cctgtcaata
    18781 atcctataag aagtaatgta attaatgcat ttagacctgt tctccctttt tcatccataa
    18841 aagttatttt agagatgtcg atattatttt tcacattatt tatatgctct ttcacttgtt
    18901 cttccatatt tcttttatat agtatataaa ctacaccaac taaaaacact cctataccta
    18961 aaaccatgtt taaattaaaa gctaccataa gcaatattga accaaaagct aactttaaaa
    19021 gcttaaaaaa tacactatct atctttagag ttatatttct gatttcttta cattcatctc
    19081 ttaaagaata tccacttgaa tctaagttat taatctttgt ttttacaatt tcagaataaa
    19141 aaagctttat tatcccattt aacattttca tcactcctaa atattttaca tatatttgta
    19201 agttttacaa ttataatatt ttttatactt ggtaataata aaaaataaca aatattttat
    19261 tatttagtat ttattgtaaa taagttattg aagcttgatt agttaagtat agatttttaa
    19321 taaatatttt tataatttag aaggaagtgg ttttgtgcaa atagaacaac tcgatttaga
    19381 aacgagaaat aaaatatact gctatactaa aaaaattctt agaaaatatc aaaaaggcat
    19441 aacatctgga aaattaacag cagataaatt tgcagataat atattgtctc aagattttat
    19501 cagtagtata cttaatgaaa aaatagtcaa tgagacaaac tttaagatat cttatagaaa
    19561 ctatatagaa actttaataa atattcaaaa tgaaaattta tctaacttaa gaaaaaaatc
    19621 tactaaaact gcaaaatgtt tcaatatctc tcaaataacc caattaaaaa acttactaag
    19681 taatactggt tataatttat taattccata taaatactta actgctagag atatagaagg
    19741 aatagttact cttattaata ctgggtctat tgaacttgga aatgagagaa tctacaatta
    19801 tatatcaaaa gtataataat ttaaacctat tataaaaagg caatttattt aataaaaaat
    19861 atttttaata ttaaataaat tgccttttta attttggatt aaatactgct atccaagtgc
    19921 tttttctagt ttagataaat gttgtattgg ataatatttt agtagctctt taaattgttc
    19981 tactgataaa ccttctgttg ttgtatatat tcttattttt tcatctaagt cactattaat
    20041 aaatttgttt tttaaatcct caaaatttac actactcatt ttacatcact ccttatgtaa
    20101 catattcatt tgtattattt tatccaaata cataaaattt attacataaa aatatatcta
    20161 tttaataatt taataaaaaa aagctatctc aatttagaaa ttactttcta tatttgagat
    20221 agctctagct aaactaatag ccaatagtta taaataatta tttataacta tctgttgctt
    20281 tttccagcca tttgttgttc agccatttca actaattttt tagtcatgta tccacctaca
    20341 taaccatttt ctcttgcagt aaggtttcct ttatcaactt gttgataatt actcatacca
    20401 agttcattag ctatttctaa tttcatttgg tttaaagcct cttttgctcc tggtactact
    20461 gttctgttgt tgttattgtt acttgccatg ttgattacct tccttctgtt taatttattt
    20521 tgtgttgctg ataatattat gtgattttaa acatttttaa ttcaggtaat ttttagcaat
    20581 taaaacctgc caaatatcca gtagaaaaag ctatttgcaa attgaatcca cctgtatatg
    20641 catctatatc tattacttct ccagcaaaaa ataaaccttc tactaatttt gattccattg
    20701 tacttgaatt tatttcgttt acttttacac caccagaagt tattatagct tcttcaatag
    20761 gtctgtatct ctttactgta aactttaaat tttttaataa atggactaag ttttttctct
    20821 gctctctaga aatttggtga acaaccgtat ttggattaat ttcacttaag tttattatta
    20881 taggtattag tttttttgga agtaaatcat ctaatgcttt ttcaaatttt ttatttgtat
    20941 acttctgaaa atctttttga actcttttat ccaatttttc ctcatctaat gcaggtttta
    21001 aatctaataa tatagtgtaa ttttctttcc ttgtatcttt cattctacaa cttgcagact
    21061 tgattatagg tccatctaaa ccaaatttag taaattcaag ttctccaaaa tcactataaa
    21121 ctttcttttg tttgctatta aacaccctta tttcaacatt tctcaatgat aatttttcta
    21181 aatcttttgt aaaactttct tgtacttcta taccaattaa agatggtttt gtatctataa
    21241 ttgtgtgccc ctgtgaaatt gcaaatttat atccatcacc agtagaacca gtaagtggat
    21301 aacttaatcc accagttgct actacaactg aatcacactt aatttctttt ttatcgttta
    21361 aaattacttt ttcaatttta ttatttttag atattatttt ttctactttc gaatttaata
    21421 atatatttac tttcttactt ttaagttgtc tttcaagagc atttacaata tcaaatgctt
    21481 tatcactctc tggaaaaacc ctttttcctc tttctgtttt agtctttact ccaagattat
    21541 taaacatact tattacatca tcatttgtaa aagtataaaa agcactataa agaaattttc
    21601 catttgtagg tacattttct attaattcct ctatttcaca agcgttagta atattacatc
    21661 taccttttcc tgttattgca agtttttttc ctaatttatg atttttctca attaatgtta
    21721 catcatagcc tttctcacat gcagttgatg cggctatcat ccctgctggt ccaccaccta
    21781 ttattactac cttcttcaaa taatcactct tttctaccct taattttttt aatttaaact
    21841 attgactcta taccttaaaa tcaatttaat tttaaaagtt taaataatgc tggagctcct
    21901 gctattccat aaaaaacaac tactgtattc ttaataaaat tcataaaaat tatactcata
    21961 gtagagtcac ttatatttaa agtgactatt aacattgaaa taaacaactt aatgccaata
    22021 tataacattc ctattgttac tatcccaacc aaaaacctac atattcttga aaaaattata
    22081 tttttactcc ccttattttt attgctaaaa tttatttttt tgttattatt atctgtatta
    22141 aatttaacaa atctatcctc aaccatatat ccaaatacaa atcctactat tataccaaat
    22201 ttttcaaaat attcagggct atttagaaat atagccacta ttataaatgg tataagtaaa
    22261 actacaagta aaatataatt tttattatca tcaatatagt caaatatttt tacaaataca
    22321 actgtaaaaa atattcctaa tatccaaccc gctatcacat ctattggcca atgaacagct
    22381 aaatacagcc tagacaatcc tactcctaaa accataactg cacctattaa atgaagatat
    22441 ctctttttaa atatagtcat aatactaacc caaaatgttg ttgcagtttg agtatgagca
    22501 cttggaaaag aataaccacc agcagtagaa actctcaatg attcaagacc ttttattcct
    22561 attggtctgg gagcttttac aaactctttg atgcctaaat ttacaacaaa atttccaatt
    22621 atagcaaaca gtattttctg tccacatttt ttatttatac accagtatat aattgttgta
    22681 attaatatta ataacggtgc ttctgtgctt attgtaaaga caagaaatat aaagttcaaa
    22741 attggatttc ttaaattttg gaaaaataat aatatattta actgaatatc catatagatg
    22801 tctcctttac taaaatttta tataattaac taaaaagcac taactccctt agaaattagt
    22861 gcttttgatt cacatttaaa tctattctac atcctcaact atacctatat aaggtaaatt
    22921 tctatacttt tcagcatagt ctattccata accaacaata aatttgtctt ctataacaaa
    22981 tccaacatag tctacaggta tattagcttt tcttctctga ggtttatcaa gtagagtaca
    23041 tagttttaaa gattttggat ttctagtttt taaagctgca actagattac ttaatgttaa
    23101 accagaatct attatatcct ctacaatcaa cacattcttt ccttcaatat ctacatctaa
    23161 atcttttaat attttaactg ttccagaaga ctctgttgag tttccataac ttgtaacact
    23221 cataaaatct atatttacat ccaaatctat acatcttatt aaatctgaaa caaatacact
    23281 agctccttta agtataccta ccactagtaa atcttcgcct ttaaagtctt cttctatctt
    23341 ttttcctaat tcataaactt tttcttttat ttgttcttct gtcaacattt ttcctgtaac
    23401 tttgtacatt ttgtatcctc ccgctttcct tctattaact tatatattat agcataccaa
    23461 aatatcaatt aaaatctttt tcatcatttt taaacctaaa ttaacagctt atccatactt
    23521 ttttgctttt tacattaaaa atttggaata acgattcctt tgtattataa tctatatgta
    23581 catatattaa tcagttattt ttatcgatta tataatttta tttttggagg tacttttatg
    23641 agcacaaacc atggagctaa tttatatagt ttatctagta agtatggctt ttcaaaggaa
    23701 gaatttatgg attttagctc aaatataaat ccttttggta catcaagttt agccaaacaa
    23761 tacattgtaa acaatataga tatggtatct atgtatcctg accctgatta catagactta
    23821 aaaacttcta tatccaatta ctgcaaatgt tctatagata atatagtact tggcagtgga
    23881 gctacagaac taatttcatc atttatacat acaattaacc ctaaacaggc tcttttactc
    23941 tctccagcct attcagagta tgaaaaagag ttatctaaaa ttaattgttc tatagaaaaa
    24001 tactttgcaa aagaagaaga taattttcat ataaatttag aaaatcttat aaaaactatc
    24061 aatgctaaag attatgattt ggtagtaatc tgtaatccta gtaatccaac tggatttgca
    24121 tttactaaag tagaagtgag agaaatttta aaaaatacag actcattttt aatgattgat
    24181 gagacttatg tagagtttac tgatacagat acatactctt gtactcaatt agttgatgat
    24241 tattctaatt tattcgttat aagaggtaca tcaaaatttt tttcaacgcc tggaataagg
    24301 cttggatatg gtcttatatc taacactaat gtcaaaaatg aaataaataa aaatctagat
    24361 ttatggaata taaacataat agcttctaaa atgggtgaaa tcatgtttag tgatttagat
    24421 tttatatcaa atactatttc cttaatgaac actgaaagag attacctgct taaagagctt
    24481 aagaatataa aaagcttaga tatatacaat actaaaggta atttcatatt gtgtaaaatc
    24541 aagacaaagg aattgactgc taaatctctt cgagagcaac tgcttccaca aaaaataata
    24601 ataagagatt gttgttcatt tgaaggtcta gatgagtatt tctttagagt atgtatatta
    24661 aaacctaatg aaaataaatt attaatatca tctcttaaag caatttttaa ataaaaaaat
    24721 cactttattt tataattttt tataccataa aaaatgagct atctctattt gagataactc
    24781 atttttgatt attaaaaaac taagcacttt gctcttcttt atttcctttt ttactcattc
    24841 catcatttat tttatctact actactgcta ttgcattatc tcctgataca ttacatgctg
    24901 taccaaaact gtcttgagtt aagtataaag ctatcattaa acttgcaagc ccaccatctg
    24961 atggtattcc aaccattggt aggaaaggaa gtgcactcat tatagcccca cctggtgctc
    25021 ctggtgctgc aaccattgct atacctaaca ttgcaatata agggaataat acacctattc
    25081 catgtggcat accattcatc attaaaactg cagttgtaaa tcctgttatt gatatcatac
    25141 tacctgataa atgtattgtt gcacaaagag gtacaacaaa ttctcttatt tgcttactta
    25201 ctccattttt ttctgcacaa ccaacattaa ctggtatagt tgctgctgaa gattgagttc
    25261 ctatagctgt taaatatcca ggtacttgat tttttaacat tcttactgga ttttttcctg
    25321 aaaatacacc tgctgctgtg aattgacatg ccatatatac aaaatgtaaa ggtattacta
    25381 ctaagtatac tttccagaat acccctaata ttgtccatac ttcccctgct atagttatat
    25441 ttgcaaaagt tcctgctatg tataaaggta ataaaggtat aatcactgtt gacaacgttt
    25501 tcgttacgat tccttgaaat tcttggaaga aattaaataa agtctctcct tttccatgat
    25561 ttcttaaagc tgatattcca agtcccatta caaatgcaaa tacgattgca gctgttacat
    25621 ctaccatagg ctctaatgga attgtgaata ttggagctaa cattcctgcc tctgggtctc
    25681 caatttgact tgcaacactt gcatcaataa aatttgggaa gattaatgta gatactgcaa
    25741 atgataataa tcctgctatt aaggtagaac catatgctat tcccgtagtt atccccaata
    25801 actttcctgc acctgttgct aaatcagcta tacctgctgt tacaaaacct ataattataa
    25861 atggtataac aaattttagg aatgctgaga ataaagtact aactgttact aatatttgta
    25921 ctattatttc tggtgcataa cttccgatta aaataccgac tatgataccg atgattagtt
    25981 tgggcactaa acctattttt ttcttcataa tatttaccct ccataacttt tttaaattaa
    26041 gcattcttgc acattgaata cttagttaaa tattggatat ccacttatta tgataataga
    26101 gttttacctc tccgtcaaca atttaaccat tttttgttaa taaaaaaaag aataacatat
    26161 ataaattgtt attcttcttc atcttttata gaaaattcta cattttcaat tatatttgaa
    26221 tgtttatctt tttttgcgat aactctatac ttattaattt cttcattcac tatacctata
    26281 ctccaaatta aaactatagc atcatctata aaaccaaatg cccctgcaat catctctgga
    26341 attatatcaa ccccagatac aaagtaggat atagtaaaaa ttaatacaga aattaccttt
    26401 attttatcaa ctatattgat tcttttatct gtcataaaat caggtagttt aagtaaatca
    26461 aatacaaaaa ccaaaccaaa tttagcattt ttaaatctga ccaaaatttt ttttaaaata
    26521 tctaaaacta tattaatgat tatttttaaa aactccatat ttatcacctc ttataattat
    26581 aggttattct acattataac cgatataatt aatttgatga acatattaga tataaatttt
    26641 atcaattagt ttatatttaa attataataa ttcaattcct gcactactac aattttgaat
    26701 catatcctgt ggccatactc caacttgtac ttctcctata tgagcttttt gtaagaaata
    26761 catacatatt cttgactgac caattcctcc accaattgta tacggaagtt cgccatttaa
    26821 aagcatctta tgatagtcaa actcttttct ctcttcacat ccagcaagtt tcaattgtct
    26881 atcaagagat tcttcatcaa ctctaattcc cattgaagat aattctaatg ctgaatctaa
    26941 aacaggattg taaaataata tatccccatt caattcccaa tcatcataat caggacttct
    27001 tccatcatgt ttttctcctg atattaatgt ttttcctatt tgagttataa atactgcttt
    27061 cttctctttt acaattttat cttctctttc ctttggtgat aattctggat acatatcttc
    27121 taactcttgt gatgttataa aagttatttc ttctggtaat atttctttta taccttcata
    27181 catactacat acaaaaattt cagtatcttt aaatactcca tacaatcttt ttacaacatc
    27241 ttttagagtc ttttcatttc tactactttt atctataacc aattcccaat cccattggtc
    27301 tacataaaga gaatgtatat tgtctaattc ttcatttttt cttattgcat tcatatctgt
    27361 atataaacca ctaccaactt caaacccata ttttttaagg gctaatcttt tccattttgc
    27421 aagtgattga acaatttctg catttttatt cataaatggt atatcaaagc ttacagcctt
    27481 ttctccattt aaattatcat ttgcacctgt ttctggcaat acaaataacg gagaagaaac
    27541 tcttataaga tttaattcat tacttagtct catttcaaaa aaatctttta atttttttat
    27601 agctatttgt gtatcaatta cgttcaaact acttttataa tcctttggta ttattgtgct
    27661 catatcattc actccctata tttatatatt tttccattaa aaaaaccctt catccctata
    27721 taaaagggac gaaaggttgc ttttccgcgg taccaccctg attagtttga aataaattca
    27781 aacccagctc acaatgtaca gatattactc gatacacccc aatatgataa cggtttggtt
    27841 ccgactaagc ctactctttt aaaatttcgg ttagcaactc caggatgttc ttcactatag
    27901 acttcatatt ggtctcacac catcaccaac tcgctttaat tacattctat aactactctt
    27961 cctttcaaag tctttaagag tttaattcaa taaaatttag ctctcttatg ttttctattt
    28021 attcataata gtatgataag tgattagtta aaatttgtca agtttattat ttttttattt
    28081 ttaaacactt tataaatttt tacctatact ttataaattt tcaaatatag tctttaatct
    28141 gtccatacat tcttttaata aagactgtgg acaagctaca tttattcttt caaatcctat
    28201 tccttctttt ccaaatatat aaccttcatc aaataataca ttagcttttt taaacattaa
    28261 atcttcaagt tctacttcat ttaatccata agcagtaaaa tctaaccatg ctagatatgt
    28321 tccttctgga taaacaagtt ttacttttgg taggttttct tttaagtatt catcaataaa
    28381 ttgtatatta ttatctaagt aatcatttaa ttcattaagc cagtcttcac tttcattata
    28441 tgctgctatt gtagctacaa tagcaaaggg atttggtgaa gttgcaactc caacagctgt
    28501 aacctcttct tgatatttct tttttagttc atcattattc actattatat tagacaactg
    28561 cattcctgcc agattaaatg ttttacttgg agctatacat gtaactattt tatccttgta
    28621 ctctgggcat aatgactgta aaggagtatg tttaaatcct tttctaatta aatcagaatg
    28681 tatctcatca gctattatcc ataggtcata ttttttacat atttctacaa ccttttttaa
    28741 ttcatcttct ctccaaactc ttccaacagg gttgtgagga ctacaaagta ttagtacttt
    28801 attgttgcta cacttagctt tttcttctaa atcatcataa tccatcctat aagtaccatt
    28861 ctcatatatc agaggattgt ttacaacgtc cctattattg ctctttatct tagcttcaaa
    28921 tgggtaatac actggttttt ggactattac accttcatta gaattagtta atattcttac
    28981 taaaatagca acagcaggta caacaccagg actaaaaata atatcttttc tacttatttc
    29041 ccagttaaat ctccttttat accaatcaca aactgcatta aagtatttat cactatcatt
    29101 tgaactatat ccaaatattt tatgttcaac ttttttacgt aatgcttcta ttattggttc
    29161 tgcacattca aaatccatat cagcaatcca aaaaggcaaa ccatcttttt gtgcttttgt
    29221 tgaacaatta gttctaaaat cccattttac acaatctgtt cctactctat ctattactct
    29281 gtcaaaatta tacttactca ttttttccct cctaagaata aaatttttat aattgttaag
    29341 tctattacta gatgcacttt ggtattattt tacaattctt aatattataa taatatcact
    29401 tttttgtcca atagatttaa aatataatta cttttccatc atattttttc aatattccaa
    29461 aatcagtttc attatatcaa aataagattg ctcatattaa ttattttcta attaacataa
    29521 gcaatctttt tctataactt ctataattat aattttaaat gtagaattat tagtttggat
    29581 gttatcccaa tctcttttca accaatctac accatatatc tacaccatat tctaaagctt
    29641 tcgtatcaaa tgttacattt ggattatgaa atcctggtgt agcatcagct cctaatccaa
    29701 tatatgtagt tttacatttt agtttttgtg tataataatg gaagtcttct ccaccagaat
    29761 ttttaaatat acctatagat gcatcagctc ccaatacatc ttttatagtt tcttcagcca
    29821 agtttacaag ttcttcatca aattctgctg ctggtactcc atctttggaa attagtacag
    29881 cttctgcatt tatagaactt acaacagttt ttacagcatg tattgctttt tcttttaatt
    29941 cttctattat atcattactt tctgccctta tatctaaagc cataaatgct tcatctggta
    30001 tattattata agtttctccc ttaactgata tatttgttac ttttatagaa tttggtatag
    30061 ctggatttac tcttattgca tttattgcat tcactgctaa acagacagca tctactgcat
    30121 ttactcctaa atgtggttta gaaccatgac aagtgatacc tttaatcgta aattccatta
    30181 tacagctagc acaatggtac attgcaggta tcgcctttcc caacctacaa tcttgtccag
    30241 gtcttaaatg catccctacc atttcttcta catcatccaa caaaccagaa tcaattactc
    30301 ttaatgctcc atataatttt tcttcacctg gttgaaaaag tatttttaac ttacctcttt
    30361 ttactaaatt tctctctttt attaactttg cagctgttaa aagcatagaa gtatgaccat
    30421 catgaccaca agcatgtatc attttctttt ctccatctat gataaattcc aatgcatcca
    30481 tatctgctct tatagcaaat actggtcctt cttttttaga atctatagtt actataatac
    30541 cagttcctgc aactccttta ttaatatttt caaaccccat tttttcaagc tcttttaaaa
    30601 caaactctga tgtcttatat tcttcaaatc ctttttctgg tatagtgtgt agataatcat
    30661 acactttatg tacatactca atataatctt tgttagttcc cataatctag ctcctcctta
    30721 aaatttaaac tttccattta tattaaattt ttattataat tacctatact acgaaataat
    30781 tcattataag cattgcaata caaccattta aagttgctat aacaaacata tgtggataat
    30841 atttagtttt taactcagct gttcctaaaa gtctccccat aaattgtatc tgcgctcctg
    30901 aaagcaataa cattggcaat agaattgcaa catgtgcttg acttatttga cctgctgtaa
    30961 ataaacctgc gattactcca gatgctcctc ccattgatat aaatattgtc attatagctg
    31021 ttgctgcaac acctggaagt ccaaagatac tcattacagg tgtaaatatt gtccctatta
    31081 catccaaaag acctgttaag ttgaacacag aaattatagc aaaaccgaag atgacatttg
    31141 gagccattga atttgtactt atttggaatc catttctagc tcctccaata aacgcatcta
    31201 tcacatttcc ttttttaact tttacttctt tttttgctac ctcactcatg ttaagcttcc
    31261 cccttgtcaa atttatttaa atataatcta agtaagttcg ccccaacaaa cttcataatt
    31321 aatattaaag caagtggtat aaatattggt acatctccta taaatggaaa tagtgctgct
    31381 ccagaagcaa agaaattcgt aattgcagac gcacctgaaa attgaaatgc acaaaataca
    31441 gctttttgtt tttcatctat atacccatca tctttaagtt ctttagtcat agatgcagct
    31501 gcatctgtag actgtaaact agctatcaaa gttagtcctg caaatccagg taaccctaat
    31561 aatggcttta atagtggact aagaagcttt tgagctgcct tcattccatc taagtggtct
    31621 ataactctaa ctactccaag tgcaaacata actgatggta ttaatgtcaa tgcatatagc
    31681 catccatctc gagggccagt acctccatca cctctaaagt ttgcagctaa tgtacctaca
    31741 ccatcattaa ttgttcccaa tgttccaaaa gaacctaata cattgttaaa atccaatact
    31801 tttaatggtc ctggtgcatc tttaaatatt cctgaaaaaa ataatgctcc caatattaat
    31861 gctacatacc cataccattt tacctcaact ttttctgttg ttgattctaa tttactcata
    31921 taaattctcc ctcctgttta aatgaatttt attagtatac ttgtttaata agcaaaactt
    31981 atgccaatac cttaatgata aagcctttgt atttattttt ttattgtaaa tttattccag
    32041 tgattaaata gcttataatt acttactttt caagtttaaa aatataatat ttttttatta
    32101 attatttaaa atatacatta caatataaaa agctattttc cttatcttgc aaaaataatt
    32161 tcaaaatagg tttttatagg ataaaatcct aaaaaaacct attatttaat caaatatata
    32221 aatttatact actatttgtt gtataatata acttgcctta tactttaaat actttaaaaa
    32281 taaatactat tttgttagga gaaataaaat ggataaaaag aagttaatcc ttataacata
    32341 tgataaattg aatagtgacc actacaaaga agaattaaca aacttttttg gagacgaaat
    32401 aatcatagaa acacaaaata tacttgatgg aataaaagaa aatttagagg gagatgtagt
    32461 gctttctcta tcacctttaa ctagtaattt tttaataaaa cattttaagg aagatattga
    32521 aattattcat ggaacgaaag cactttcaaa acttggatat gaaaaaatga tgaaactgcc
    32581 tccaggtaca aaatcgttac ttatgacaac caataaaact tcagcatttg agatggctac
    32641 ttatctatat aaaattggta taaatcatat agattttgta ccgacatatc cagattgtga
    32701 tgaaatatat gatttagata cagctataac accaggtcag attagattta taccaaaata
    32761 cataaaaaat attgttgatt taggttggag aaaaatctca ttagatacat atatgtcatt
    32821 attagtagta ttaaaactaa aaaatgagaa atttatagaa aaattataca aattgtccaa
    32881 ggagacacta agtcatgatt tcttaaatac atcattagat aatatatcta aattaaaaac
    32941 tatactttac atgaccattg atgaaatagg tgatggtcta atatttttca atacctttaa
    33001 taaggttact tttgtaaata agtctttact taatatgttg gaactagatg aaaaacttat
    33061 aaaatcacct tccttaatgg aatatatgcc aaagagtttc ttagataaaa ttacaaagaa
    33121 cttaaatata gataatatga ttatatacat agacgaaata gataaaaaat tcatactttc
    33181 taaaaaacca ttttatcttt ataaaaatat agagggttgc ttaattacat taaaagatgt
    33241 aaacaatata gaaatattag aacaaaaaat aagaagtgat tctgtcaaaa gaggttatgt
    33301 agctaaatat aaatttaata atattattgg aaatagttct attataaaag attgtattaa
    33361 aaaggcaaaa aaaatggctc ttacagataa tcctatatta ataacaggtg aaacaggaac
    33421 aggtaaagaa gcctttactc agtcaataca caatcattca aatagaaaaa ataaaccttt
    33481 tgtagcaata aattgtgctt cattaccaag tgaattactt gaaagtgaac tatttggata
    33541 tgaagatggt tcttttactg gagcaaaaaa aggtggaaaa aaaggattat tcgaattagc
    33601 tcatactggc actatatttt tagatgagat aggagatatg ccacatgatt tacaggttaa
    33661 acttttaaga gtcttacaag aaaaagaaat tagaaaaatt ggtggtacaa gtattattcc
    33721 aattgatgtg agaatattag ctgccacaaa taaagattta gagaaattaa ttatagaaaa
    33781 taaatttagg atggatttat tttataggat aagtatgttc actttagatt tacctcctct
    33841 tagaaagaga ttagaagata tacctcttct tttagaatca tttttaaaag aacttcccta
    33901 taaaaacata aaactcgata agagtctttt agaagcttta aattcataca cttggatggg
    33961 gaatattaga gaactgagaa attgtgttga atatatggct tacatgggtt ccaattatct
    34021 tacaataaat gatttacctc aaaacatatc ttcaaaatta aataataatc atatgtcaag
    34081 taatatgtcc atattcaatg atttaaacca atatgacaaa aacatatgca tttctatcct
    34141 aaaatcattg cacatgaaac caatggggag gacaaagtta atgaaattta tggaatataa
    34201 tgtcacagaa tacgaagtaa gaaatatgct agaatatttg acacgcaatg gatacttaat
    34261 ttctagtaag ggaagaaaag gctcatcaat aactgagaaa ggcataaaaa taatagaaaa
    34321 taatataatt taaacctata gtaattcatc tttactttat caaaatttat atataattat
    34381 aattttttat ttttctataa attttttata aaataataaa acttatttat attaaaaacg
    34441 aagctatctc aacatatgga taagcttctt tttaattgtt aaaaagataa aaatatattt
    34501 gatttaacag aaaaagagtg cctcataaac taaaaagttc attttgagac acgtccttaa
    34561 tattaataaa ttaattggct taaaattact gattgataat tataaagtac ctctgttaaa
    34621 ggagtaaatt ctgaaccgat tccaaatctt aattctgtaa acgcctctaa ataatctggg
    34681 tcacatacat cttcttgtgt taaatcttgt ttcaccattt cagtagcaag ataaattgct
    34741 actgatggta gaaaagcatc cattggcata ggatatccac caagtctatt tgacatgcca
    34801 aaagccttag ccttttcact tatcatgtcg tttattttat cataattttg agcatctttt
    34861 tcagatattt ttagacccat aacactagga tatgtttgta ttggtgatgg attgctttgc
    34921 tctgcaacta tatactttaa ttctaaagcc ttagtcaata taacttcatc catatactca
    34981 tttacaccaa atacatttat atcttttcca tattttttaa cttgtttttc aatatcttca
    35041 tttaaaaact gcttaacttt gtttttgtct tcttcagtat ttatatttgg agtattaact
    35101 tgtacaaatg gcaatcctat atttttgcaa gtctctttaa tcatttctaa tcttttggct
    35161 atattaacat ctttcaaatc atctgtagag gcataatgaa taaaagcttt tgctcccatt
    35221 tcttttgaac gttcagctaa tacttttcct ctttcctctg cactaacccc tagattcaca
    35281 tcaacaaatt ggcttaattg gtttttatca tctcccatag gggcagaaat agttattatt
    35341 tctggacgct tttcctttac cttttgtaaa gcaggtaaaa gccctgcttg gtcagttgaa
    35401 actacaatag cctgaacttc tttatcgtca gccaacttta ctatcttatt aatagcactg
    35461 tctatattag aagtaaaatt ctctggcaat acaacatgct ttatagtttg tttaacttta
    35521 gtttgaccat ctttgtcttt atcaatttta ttctcttcct catattcttt tgccatctct
    35581 tccaccatat tgtattgtac tttattttca gataatggtt gagttaccac tgcaacttta
    35641 aaatcatcta taattttctc tttatctttc ttgcctacaa gtaaactaca acctgtaagt
    35701 ccaataatta aacatatgga tagcataaat gctgttattt ttctaagcat taattaaaac
    35761 ctcctaaatt tgtacattct tatcactatt actaatttta tcatacttca aagaaaaatt
    35821 atattataaa aaattatata catgctatta taatccaatc taaaaagtac tatgggtaga
    35881 ttaggtttag attaaaccta cccatagtac taaaaattaa tatattataa aattaaatta
    35941 attacaaatt attatgctac ttgtttgttg ctttttataa agttaccaac tattccaccc
    36001 aatatagctg gtattaccca gttaaagccc aaacctgcaa gtggcaagct ttgtataaat
    36061 ggaacagctc ctgtcattcc atttatagct gataataaac taatcactaa tgtaacatat
    36121 gctgcacctt taaaagcatt atcattcttt atatgtttac cagccaactc cataacaact
    36181 attgctaata aaacaggata tatagtttgt aggattggaa ctgatatggc tattatagaa
    36241 tcaacaccta aatttgatat aagcatacta gctacacata tacctataac cagttgttta
    36301 taactcgcct tacttattcc ttcaaaataa gttgatattg atgctgttaa acctgtagaa
    36361 gtagtcagac aggcaagtgc cacaattaca cctaaaatta cctttcctac ttgaccatat
    36421 aacaatatac tagttatttc aactaataaa gatgcttgag gtatactagt atcatataaa
    36481 gttgaaactg ttgcacctaa ataagtaaga ccaccatata ctactgctag aaatacacag
    36541 gctacaaaac cagctttaat agtcattcct atttggtctt tatctgatgt atatccttta
    36601 gctattatag acatcaacat tatagttgcc atagatgctg cacctagagc gtccattgtt
    36661 tgataacctt gtgatatacc ttcaccaaat agatttttat ctatcatatg ctcaggactc
    36721 ataggtcctg ctggagaaat tatacccttt actattaaaa caaataatac taataataac
    36781 gctggagtta aatacttacc tattatgtct ataaccttag aagacctaat agtaaatgct
    36841 aaagttagcc caaagaagat tattgaaaat acagctttag ctactggacc atggtctcca
    36901 aatataggca taatacccat ttcatatgtt gttgcgcaag ttcttggtat tacaagaagt
    36961 gctaaacatg ctagagcagc tacactcatt actcttgcta accctttacc agctctccta
    37021 agtacatcat ccatgcttcc cttacatctt gctgttgcta taataacaag cattgctata
    37081 ccagcatctg caacaacaaa tccaataaga ggaattaacc agtctttgcc ggattccatc
    37141 cctaaaaatg gaggaaacat aagatttcct gcaccaaaga acatagaaaa taaagcaaaa
    37201 ccaactataa aaatatctct catctgctta ctcacactaa attaccccct ttgaaaatat
    37261 tttgaatatt aagataatct tactttattt tcagaaaaat gtcaatctgg taaaaattca
    37321 cacattgtta acgttttccc aatttatttt atgaatattt ttttcatttt atgataattc
    37381 ccatcaattt cgttaaatca attgcttgtg acacatcaaa aattgctcct ctaatatcat
    37441 ctataccaat ctcaattccc tcaatattat tagtagtaaa atctatgcca gaaagtttag
    37501 ttccattaaa gtttgctttt tttaaatttg tatactcaaa tactagattg tcacctttta
    37561 cttcttgaaa tacagaattt tcaaaatttg aatctaagat agaaactatc ttaaatttag
    37621 agaatgaaaa gttagaatac ttacctaaaa catccttgaa caaaacgttt ttaagtatac
    37681 aatcatcaaa tctaactcct atcaatttac tatttttaaa ttcaactctg tatatacttc
    37741 ctccatcaaa aaatgtattt gataaattgc agttttcaaa taccacatca agtaaatcaa
    37801 tcttttttaa attacaattt tcaaaagaca catttctaaa tatacaagat tcaaagctaa
    37861 ctcttacaga atctaaatca cttataactt cattttctat aattttatta aatatttttt
    37921 catcattaaa tatatcttca agtatatctt caagattttc aataatttct aattctcctg
    37981 taaatttagg tttttgcact ttaataattt tagtcatttt cccctccact ataaattata
    38041 ttatttttga tattattttt tttaatatat taacttaata tacatatttg atatattaaa
    38101 aagtttttac gaaatatcca tgtattcagt acttttataa ttatatcaca aactcattta
    38161 taataaactt ccattaatag tataaaacaa gttagaataa ttgacattat actcatacat
    38221 attcaattat tggaaaaagt aatttttttc aataaataac cttaattttt tataaaatcg
    38281 acaaaaaaac gaataatttt attatattta ttgtaaataa tacaatatta cgatataata
    38341 caacagaaaa acaaatagaa aatgttcata taatattagt aatacggtct aatagtttct
    38401 acaaactcac cttaaatggg ttttctatga atagaatgtc aactctgaat atatgttcta
    38461 ttctatctat atgaatatat atttttttgt ttatatctta aagttttttg gaggtaaaaa
    38521 tggtaccaaa cactaacaat gtacaaccag gtggaaataa ttcatcatta agctttttag
    38581 acaaaatgtt taaatttagc gaacgtggct ctagccttaa aacagaagta ataggtggag
    38641 ttactacatt cttaacaatg gcatacatag ttttcgtaaa tccagcaata ttatctgaag
    38701 ctggaatgga taaagccgcc cttataacag ttaccatact agctacagcc attggtacac
    38761 ttatatttgc gattttagga aatgcaccat ttgctctagc tcctggtatg ggattaaatg
    38821 catttttcac atactctttg gttataggtg caggaatccc atggcaaaca ggtcttgggg
    38881 tagtttttct atctggatgt gtcttcttta cactcgcagt aacaggtctt cgaaagacaa
    38941 ttgcagacgc aattcctaga gaattggcag tagcatcttc agcaggtata ggtcttttcc
    39001 ttgcttttgt tggtttaaaa aatatgggtg ttataatagc taatccagat actatagttt
    39061 cacttgcaga ttttacacca acagttttaa tagccctttt tgctcttatc ttaatggcaa
    39121 tactagaata taaaaaagtt aaaggtggaa tattgataag tatagtagtt gcaacaataa
    39181 tatctgttat atttggattt gtggagttgc caacagcaat aatctcaaca cctcctagta
    39241 tagcaccagt tgcttttaaa ttagatattg taggtgcatt gaaattctca ttaattggac
    39301 ctatattctc tttcatgttt atagacatgt ttgattcatt agcatttctt attacttgtt
    39361 gtaaacaaat gggtcttgaa gatgagaatg gtgaaataaa agatttagga agaatgttat
    39421 atgcagatgt ttcttctact ttaataggct cattcttagg aactagtact gttacaacat
    39481 ttggagaatc tgcttctggt attgcagcag gtgcaagaac aggacttgct tcaatagtaa
    39541 cagcagcatt attcttagct acacttatat ttacaccatt acttggtata gttcctactt
    39601 acgctgcagc tcctgcactt gtaatggttg gtgtgtttat gtttgaaagc ataaaacaga
    39661 ttgattttgc tgatagcaaa atagctgtac ctgcttttgt tacagtttta ttgatgcctt
    39721 taacttatag tataagtatt ggtttatgct ttggatttat ttcatatata ataatgcatg
    39781 tagtagctaa agaaactgat aaaatcagta ttacactttg gattataggt ttacttgcag
    39841 ttgtaaactt agttctaagt taatattttt aaatagttct aggtcaatat tttaaattat
    39901 atagaataaa atcttattta ttttaaattt ataaatagca aaatcacttt atatgtaaat
    39961 tgattttgct attaactttg tatagaaaat ttattatcat aatatataga tataaaaata
    40021 tatagaaaag gagtggtgtt tatgtcacaa ttacttataa aaaatgcata tctaattact
    40081 atgaatgcag atagagaggt atatgaaaat ggaagcatac ttattgaaaa taatattata
    40141 aaagaggttg gtaagtttga tattaatatt gtagataaag atgcagagat atatgatgct
    40201 aaaggaaaaa tactaatgcc agggctagtc aatactcatg ttcacttatc tcaacaatta
    40261 ggacgtggtc ttgcagatga tgttgtactt ttaacttggc ttagagaaag aatttggcca
    40321 tacgaaagta gcttcaatta tgaagattcc ttgatttcat ctactgcatg ttgtgttgaa
    40381 ttgataaaat ctggtgtcac tacattttta gaatctggtg gtcaatatgt agatgctatg
    40441 gttgaagctg ttgataaaac tggacttaga gcctgtttag ctaaatcagt tatggataca
    40501 ggagatggtc ttcctgaggc atggcaaaaa actactgatg aggaaatcaa cactcaatta
    40561 gacttattcc ataagtttaa taatacatct aatgagagaa ttaaaatatg gtttgcatta
    40621 agaactattt ttaatacaac tgaagaatta cttgttagaa ctaaagaact agctgataaa
    40681 tacaatacag gtatacatat gcatattgct gaaatagctg atgaaatttc atttgtaaag
    40741 caaaacaatg gagttggaac agttgaatac cttgataaac taggtgtatt aggaccaaat
    40801 cttttagctg ctcatacagt ttggcttact cataaggaaa ttgatttatt tagattacat
    40861 gatgtaaaag tttctcatga cccaggttca gctatgaaag tggttctagg gtttgcatca
    40921 attcctgaaa tgttagaaaa aggaatacct gtttcaattg gtactgatgg agctccttca
    40981 aataatagaa tggaccttat gagggacatg tatcttacat cactaataca taaaggaaga
    41041 actttaaacc caactgtcgt accagctgaa gaagtcttag aaatggctac tataaatgga
    41101 gcaaaatgtg ctttactaga aaaagaaatt ggtagtcttg aagtaggaaa aaaagctgat
    41161 atgattattc taaatccaga tacaattcat tgtttaccaa tgcataatcc aattggaaat
    41221 atagtttatt ctatgactag tgaaaatgta gactctacta tttgtgatgg taaatggttg
    41281 atgaaggaaa gaaaagtatt agttgtagat gaatctgaat tattaaataa agttaaaaaa
    41341 caagcttcta aaataaaaga taaagcagaa gttaaaattc catctaaatt taaaattata
    41401 aaataacata aatttcaatt gttttgtaaa tatgaatata taaatatatt tatatacatc
    41461 atatatacaa aacaattgtt tttttatttt atattcatta gcattgctca taaaatacac
    41521 cataattcca gaaaaaataa ccaatcagtt aggattacat catctaagca atatataaac
    41581 gtcttagttt ataagtttta ttctactctt atctaaaacc atctttaaag agttttcaac
    41641 tgtttcaatt acatctttat aactcaagta cacatttgcg tagatacaat cttcatataa
    41701 attatattca gttactctac aatacagcag ttttcctcca tctaataaat tgctcaaaat
    41761 atagttttct tttcttggga catagcctat cacatatcca gtttcaccaa ctattttaat
    41821 agctttttca tcatatagat tatctttctc tctagttaaa tagtatatct tacttttctc
    41881 caattctttt tcaagatatt ctctatttct aaaccttgaa ccagcaacag ctattgaagt
    41941 caaatagata tccttaccat gttcatctac ttttattttt tcaatattta caaattcttt
    42001 ttttaaatta ttactttcat ataatactct tcttaattca tccttcaata taacattttt
    42061 ttctttttca tacgcttctt taacaatttt taattcttca agagatagtt tctctctgta
    42121 tctttttaag atatttatgg attcttttct tacttcattt atatttccaa atattccttt
    42181 taaagatata ttctttcctt caggatatag tgatttactt cctttaagca caataaaaaa
    42241 taacatatca tcataatatt cttgagtcaa tttatctttt tctatatctt tcatttttcc
    42301 aattaaatca tctattttaa acgtctcttc aaaaaaattt aataatttta atctagaact
    42361 tgtcgttccc tttttagata tatgccaata tacctcaaaa tctctaatat ctctatttag
    42421 atatggcaac aagtcatcaa aagataagtt tacatcataa atttctgaca tttttataat
    42481 atcttctgtt tttccactag catctcttaa tgcttcaaaa tatatatttt tccatttttc
    42541 actgagcaag ataccattta tttctttttc aaattcaatc ttattgcact ctatattttc
    42601 atcttttaag acgtttatag ctattaggaa aattgaatat aaactttcaa agttagtccc
    42661 ataatttact acagtaggta aaaatcgatt cactaattct aatttattac ctatatattt
    42721 aaattctaca cttagcaagt aattgcatat aagacgtgct aaattattta tcttttcttt
    42781 atctaaatct cgtttttcaa gatattcatt caaatctaca attgaaataa tatagttcat
    42841 caataatcgt tcgtactttg tatccttata accatcttca attagatagg aatttatttt
    42901 agaatctaaa ttttccatat taacaatagc aaatactttt attgaacctg tagccttttt
    42961 agataaatta aataaatatg tattgtaaaa ttcaagtttt ttaatagtat tactcagata
    43021 aaatacgtat tctccagatt tagaaaatgt atctacaact tctcttaagt tttctacatt
    43081 tagatatttc tcacttaaaa ctaaccctag ttttacatac tcaacttttt tactcttata
    43141 tattaattct ttagttaatt tatataacca tttctcatca ataatcattt cttttaccaa
    43201 agtatttatc tttttcataa aaatttcgaa ataacaaatt aaagacttag aattactacc
    43261 ctccaataaa taaatttcat tagattcact tttatacata tttaaaattt catctactag
    43321 attatttgca agctctactt gttctcttaa cggtatactt tcatatatac ataggtaatt
    43381 catttcaaat gattctttgt atccatcata accattaaat tcctgcaatc gcttaaaaac
    43441 attctccacc aaattatcct cctttacatg atgtaattta tattgattat actacttatt
    43501 tcatcaagtt tacatttata cgcaaaaaaa tattactaga aattgaaatt ctagtaatat
    43561 ccttattatt aattatgtaa ataccctatc ttataactgt aaataatatc cttattaatt
    43621 ttaattactt ttaataaaaa tctatcctag atttagactt ctctatgcat atattatact
    43681 aatattagac atcataatca tacacttttt ctttttcata tctcatgttt acaaacaatg
    43741 caagtaaaat tccaacaaat gttattgcca cattaaacaa tacaacatat tttggtccat
    43801 taactccccc tgattttgta ataatacctg ctatatttaa aataacatag tttgctatac
    43861 tagatgcaat cataactata gatgttattt ttcctttatt agttggaaac atttcatttg
    43921 cagttgacgt tgttaactga agaacacctc ctgctgctga ataacctatt acaaatcccc
    43981 ctaacataca aatagttgga gtttgaacaa aatatacaac taatagcata agtaaagcta
    44041 ttataggata tataactaat attcttattg gtttaatata ttttttaata agtaaagaag
    44101 ttattaatac tgcacatatt actcccattg agtaaaagga ttgaattttt gtagggtcag
    44161 ccatcccata caatttaccc aattcctgat tacaatttaa ccataataca aatgtagatg
    44221 tacatgtaaa tccaatacaa acaagagcta tacttgttgg tgtaaatttc atcttctctg
    44281 atttaactgt tttgcctttg ttatcaataa cattattagc tggtggaaat ggtaacacag
    44341 ctatcaaaaa ggcatctact acaataagaa tcgctgttac tataaaaata actttaaatg
    44401 atattgaatt tgctgcaaca aatccaatta taaaaggaag caaaaattgt gctaaagcta
    44461 ttgtaaattt agtaaacata tttgctattg ttcctaaact agcaaatatc tccatacaag
    44521 aaggagttac acaggtatct aagaatgaat ttgctgcccc tccaattatt gcaaatacat
    44581 atgctatata aaaatttggt gaaaatacaa ttccaacaaa gaaaattgca tgtagaaagt
    44641 ttcctataag accacttact tttcttccat acttatctga aataggtcct gcaaatggat
    44701 aagatataag tctccctaat cctagtgcag caataacagc taaaacaata cttacatcca
    44761 atgttccatc tgataatgtt ttagcccccc acattgatga aaattctggt ttgtattgtc
    44821 ctaaaattga aattccaata ccaagtaaaa aatatgaaaa atacaacgct atggcagtag
    44881 gataatattt tttattgttc ataatttttc tcccattctt gttaatttat taacattttt
    44941 gtgtacaata aactcatccc cataatggat ttaaagtata gtcagttaaa gcttcaaatt
    45001 taactggact atgaggcaaa acccactatg acaatttgta atttattatt aaaaatattg
    45061 aaatacgtat gttttttcat tgtttaagat attactctta tctatttaaa gcataattca
    45121 ttaacttctt ctacaggcat ttctaatcca gtatatagat taaaagcttc tgctccttgg
    45181 tataataaca ttcctaatcc accaatagcc ttacatccat tagcttcagc atcttcaatc
    45241 atttttgttt tcttaggatt ataaacgaca tctgttacta ctaaatcttt tcttaaaaca
    45301 ctagtatctt ttatatttgt ttcattgtca taaggcttca taccaataag agttgcattt
    45361 gtcaatatat cacttgattg tatctcctca taaagtttgt ttgtatcttc taaatcatat
    45421 agattaacaa cacattctgg tacatctttt tttatattct caactgtttg ctctgctctt
    45481 ttataaaaat catccttagg attaaagata gatatttctc ttgctccatc taaagcacat
    45541 tgtacttgta tagcagttgc tgcaccacca gcacccataa ttgtaatctt tttaccttta
    45601 acttctaccc cattttcttt taaattacga acatagccta ctccatctgt tatatgacca
    45661 actaactttc cattgtcatt tacaattgta ttacatgctc caattatacg agctgctgga
    45721 gaaagttcat ccatatattc tgtaacagca gatttacatg gcatagtaac attggctcct
    45781 tttatattaa aagtttttat tgctaaaagt gcctctttta ccttatctac tgtgatatca
    45841 aaggctaaat atctataatc taaatctaat ttcttaaaac tataattata catcactggt
    45901 gatttagagt gaccaaccgg tgtacctatt aatgcaaata atcctgttct acctgaaatt
    45961 tccataacta taatctcctc tcaatattat cattttcttt gcaaataacc tattcagtta
    46021 tttacaaaga aaaatttatt tattatttat atatttaatg ctacttcaaa agcttctttt
    46081 gttgcatcta atgccctacg agcttttact gcatcaccaa caatataagt ttcttttaca
    46141 acttctttta taacttcact taatgggtcg taattacgag aacccatagc aagtactact
    46201 gaatcaaaac cttttaaatc atgctctgtg ccatctgtaa gtaaatagtc gacaccatca
    46261 tcataaaatt tagaaacttt tgcaccagta atggtatgta tattataatt ttcaaaatca
    46321 ttcataagga atttacggtg ttctgaaatt acatctgcac caacttcttc tctaagctca
    46381 ataacagtaa cctcatgttc ttgttctcca aggaaggctg ccacttcaca tccaaccatt
    46441 ccacctccaa caacaagtac ttttttccca caagattttt taccatcaag aaggtctaca
    46501 gcatgaatta atcctgaatc attgattcct ggtataggaa gtacaagtgg agttgctcca
    46561 gtagctataa taactacatc aggtgatttc tcctttataa gttctactgt agcttctgta
    46621 ttagtacata tttttacacc atattgatta cattttgaaa tataacttct aactaagtta
    46681 gtaatatctc ctttccctgg aggatatgca gctaaacgca tttgcccacc aagaacttca
    46741 gatttttcaa ctaaagttac attatgtcct cttttagcac atacccatgc tgcaagcata
    46801 cctccaacac ctccaccaac aacaagaact tctttgctaa tttcagctgg cttaagctct
    46861 gcttcatgtc ctaatatagg atttgcaaga caggctatag gtttgccttg gaacatattt
    46921 ggtacacatc cttgtaagca tgcaatacat ggtatcattt cgtctagttt tccattttta
    46981 gctttattag gcatttctgg gtcagccaag ctttgtctac cgaatgctac taaatcacat
    47041 cttccttgtc tgaccattaa ctcagcaaac tgtggctctg tataacgtcc aactgttatt
    47101 actggtatac taacagcttt ttttatttca gtaactaatt ctgcactgaa tccagcatgt
    47161 aaagtcgtag gagcccacat atattcatca tgtatatgta tactacgtga aacatgaagt
    47221 ccatctattc cacaatcttc tagatatgct gctattactg aactatcgtg tacatcaatt
    47281 cctcctggaa catcatcagt gctgtttata cgacataata ttgctagaga atttcctact
    47341 tttttacgta tattttcaat gataagacga ggtaatctca ttctattttc aaaacatcca
    47401 ccaaactcat ctacacgctt atttgttctt tgtgatataa aactactaac cagatatcca
    47461 tgagcgcagt gaacttcaac agcatctgca ccagcttttt gtgcacgtaa tgcagcatct
    47521 ccataagatt ctataagttc atatatttct tctgttgtta ttgctagtgg tgtattacga
    47581 ccatttgatg caggtattgc agaagcagct tttaatggat gtcctgctac tttttcattt
    47641 ccttcagaac cagcatgttg taattgtata gataccttcg aacctgcatc atgacaagca
    47701 tctattacat ttttaaaact atctattgtg ctatcatcat ataaacaagg cttacgagga
    47761 cctccttttg cttttttgtc tacaacacta gattctatag ttattaatcc aaaaccacct
    47821 aaagcacgct caagataata agccttagaa gtttcactta aagtaccatc tgtgtttgca
    47881 aaattgtttc ccataggggg aactacaaaa cgatttggaa ctgtcattga accaattctt
    47941 ataggtgtaa acatagaact aatcttcata aggttttcct tcctttcaag ttttatatta
    48001 taatactctg tatattttta tattatataa gtttattttg gagatacttc agaccatgct
    48061 ttatccaaaa cttttttagt tgcattaaat acagtttcag cagcagtgga taacccttta
    48121 gatattagag aatcagatat tacttctgcc cctatgactc ttggtgatat attgtgttct
    48181 tttattattt tcacaaattc ctctgtgttt ccatatcctt cacctggagc caaacggtca
    48241 tgtagtgatt catccctaag tttttcataa ggagtttcta aaacatcaca cacttgaatt
    48301 gatactatct tatctgctgg aacaggtgca aggtcctctg ctgtctgctt tgctctagcc
    48361 cagtgccaag tatctaatat aagcattgca ttttcacgat tacatccttt cacaacacgc
    48421 catgcagtag ccaaatctgg tacaccacta tatggcataa actcaagacc tataataagt
    48481 tcacctgctc tgtcacataa ttctcccaat gcagttataa tttgttcttc tggtatcatc
    48541 tctaataatc cacaatttat atgtttaaca ttaaataagt gagccatatg ataaacattt
    48601 tgctcttttt ctctttgagc ttccgttcta tcttcttctc ttccccattg agttatatat
    48661 tctacttctg taactttcat atcatattca ttaagaattg caatcatttc ttcatctgta
    48721 acacctgcat tttttgcatc aatataattt tcagcacgca atccaatacc atcaaaccca
    48781 gcttcttttg caatacgaac tctatcacgg aatgaaactt ctgttcccaa tgtataagaa
    48841 ctaattgtaa ttggaatatt aagatttttt tgattattca tagcttaaat ttgccccctt
    48901 tattttcttt ataattaata gtatttttga ttttatattt ttatttatcc tactagtagt
    48961 attattttac cttaaaaata aaagtatttt ttgatttcta tttatctaga ttttataaat
    49021 ttattgtatc acgagacttt actaataaca aattgataat ttcctattaa ttcatagatt
    49081 ttgtttatag attttattta taagataatt c
//
LOCUS       DAMJYR010000016        48792 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.016, whole genome shotgun sequence.
ACCESSION   DAMJYR010000016 DAMJYR010000000
VERSION     DAMJYR010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 48792)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48792)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48792
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.016"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            208..1425
                     /locus_tag="PC471_000889"
     CDS             208..1425
                     /locus_tag="PC471_000889"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase"
                     /protein_id="HDF2788029.1"
                     /translation="MMNVTLTDVKEARETIKNIVKRTDLLESVKLSEKTGANVFYKCE
                     NLQKTGSFKLRGACNKIASLTDEEKANGVIASSAGNHAQGVALGAKMTGIKSTIVMPA
                     TAPLAKVSATKGYGAEVVLNGAVYDDAYAKAVEIQKETGATFLHPFNDKYVIAGQGTI
                     SLEIFEQLDNKVDTILCPVGGGGIISGVAVAAKALNPNVKIVGVQTANIPSMKESIKN
                     GKVTTAFNDTTIADGIAVKTPGDLTFEIINELVDEIVVVEETEIAESILFMMESQKIV
                     SEGAGAVCTAAILSGKYVPAKDENVVCIISGGNIDINTLYRIIGVALAKEGRRYSFST
                     IMEDKPGNFAELTRIISENGGNILSANQGKLSAGEALGKQSAEFILETIDYDHIARIK
                     KAIEEKGFKIIEL"
     gene            1520..1900
                     /locus_tag="PC471_000890"
     CDS             1520..1900
                     /locus_tag="PC471_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HDF2788030.1"
                     /translation="MKHEVIHTNDAPAALGPYSQAIKAGNLLFVSGQVPLVPETMEVV
                     EGDVQAQTAQSLKNLKAILAESGADFSNVVKTTVFIKDMNEFGAINEVYAEYFGENKP
                     ARACVEVARLPKDVKVEIEVIAVL"
     gene            2184..2666
                     /locus_tag="PC471_000891"
     CDS             2184..2666
                     /locus_tag="PC471_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDF2788031.1"
                     /translation="MLNFFKKNKSYKLHAVVSGNSINIEKVNDSVFSKKLMGDGVAII
                     PNSNVVVAPCNGKVTVLTESKHAFGMVSDEGVEILVHIGIDTVSLQGEGFKNEVSQGD
                     TVKKGSPIISFEREKINSQGIDCTTIIIVLNHSEFSEINCMVENEVVAGQDTVIEIMK
                     "
     gene            2687..3502
                     /locus_tag="PC471_000892"
     CDS             2687..3502
                     /locus_tag="PC471_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDF2788032.1"
                     /translation="MRVVKIFNNNALSTVTNDRKEAILLGLGIGFNKRPGDKVNEDKI
                     EKIYYVQDHMQTKFLELLKNVSPEVMDASQQIISLIPGNEGKFNNKGILSLVEHISFA
                     IDRMKNNVFLPNLMLLDIKMMYSKEFELGVKALEIIYRVCHIHLPEDEAGYIALHFVN
                     LQSNDNLAYDTLKFVKGSIDLIKECYGLELDESSLSTLRFRTHLKFLAQRIFKNEICQ
                     DDKMIEMYDYLINNHPKNKEYLEKLNVYIEKEFRYKLDKPEKIYLLLHLTKIL"
     gene            3553..5103
                     /locus_tag="PC471_000893"
     CDS             3553..5103
                     /locus_tag="PC471_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDF2788033.1"
                     /translation="MKEKLLGGLETFAKAMVQPLMYLSSAGILMVVGVLLTNDMLLGV
                     LPFFKWEPIQIFGSLIYNCIMAIINNLAVIFAIGIPAALAKKEKHKAALIGFMSYIIY
                     LTASNTMLTSLGQLAKESKMLGLIGTGQATILGIQVVDTSVFGGIILGCIAGYVFNRT
                     SNKTFKGALQIYSGANFSFICMFFVSILIGVVSNFIWPLAQQGIASLANVIKDTGTFG
                     LFLYGFLERILVPTGLHHLIYTPFQFSDLGGVLKVGADTVSGAYAIVMTEMNLPVERF
                     SDSIYYMATGFTKMFGYIGIGAAFIYTAYKQNKKKVKATIIPLIITAVLASVTEPLDF
                     MFVFCAPFLFFVHAVIAGLFIALLKIFDVTALCGGNLLVSIIMNTTVGVEKTHWPVMM
                     ILGLAQIIVYFVVFSFLIKKFNYKTPGREDESTATGEEQSKQLNLDAGIENIIDGLGG
                     KENINTVENCITRLRVNVKDESKINEDIINLTPNSGIVRKGKDIQIIFGLHVHEVRRA
                     VEDFLEAY"
     gene            5200..6552
                     /locus_tag="PC471_000894"
     CDS             5200..6552
                     /locus_tag="PC471_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-alpha-glucosidase"
                     /protein_id="HDF2788034.1"
                     /translation="MIITIVGGGSTFTPGIVKSIALREKELELEEIRLFDIDKERQDK
                     VAVVVNWILKEDVKTDVKLTVTNCEKEAYTDATFIFAQMRVGKYEMREQDEKIPLKYG
                     CVGQETCGCGGMAYGLRTIQPMIKVIDDVEKYAKKDYWILNYSNPAAIVSEACRKLRP
                     NARIINICDMPIAIIDMIAGSLNINDVHNIRYDYFGLNHFGWFTSIDYKHRDLMEEIK
                     EYIKENKILLPKSYIENLNKSRSTGNRHAVSSWIHVWESVYTMLESFPKYLPNTYLTY
                     YLLSKETVEHSNINRTRANEVMESREKDLFEGVRHYLETGEISEKAFYAGSHGDWISD
                     LAVSIKNDTRSRFLVITENRGAIPNMPYDAMVEIPAYIGKDGPEVIARGPIPLFQQGL
                     MMQQLNSEKLIVEAYIENSYEKALQAFTLNKTIPSMNVAKEILDEMIEVNKEYWPELR
                     "
     gene            6683..7441
                     /locus_tag="PC471_000895"
     CDS             6683..7441
                     /locus_tag="PC471_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2788035.1"
                     /translation="MNIINKIFWEEDRIGMITNNLEADTHSHCMLQLFLGIEDSIEIT
                     VNEKLVKCNCIIVDKNISHSFSARKKVYYSAIIEPTSIYAEQLTSKMNDFGYWICDND
                     GLEKLRQQGTFLIDNSSKEQYLRFMEMLNNYLNIPITLKHYDDRITELLNLLRTCNCD
                     NHTISSFADKVSLSASRLSHLFKEQIGIPLKSYILFHQMECAFRELLSGKNVTEASMT
                     AGFDTPSHFAGTVKRMMGMPVSLSLKDSEFLKVY"
     gene            7488..8207
                     /locus_tag="PC471_000896"
     CDS             7488..8207
                     /locus_tag="PC471_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HDF2788036.1"
                     /translation="MNKEYLEETKMLNYNEPQLKLLVSSKNWLELDDFHKIKSIYEFV
                     QNDILFGYNASDMLSATQVLNDGMGQCNTKATLLMALLRAVNIPCRLHAFDVTKDFQR
                     GATSKLISLLAPKYILHTWVEVFYQDRWIALEGVITDKKYLEAIQKKFFNHGGTFKKY
                     AIATNDLKNTSIDWDGKDTFIQKEAIVYDYGIFPSPDVFFSTHSQHMSKLKNFIYVHL
                     IRKIMTKNVCKARNNYIDKNE"
     gene            complement(8331..9731)
                     /locus_tag="PC471_000897"
     CDS             complement(8331..9731)
                     /locus_tag="PC471_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HDF2788037.1"
                     /translation="METQSNNKKGNLIIAIVMTGAFISSLSQTLLSTALPNIMSDFKI
                     TADIGQWLTTIYLLIAGIIVPTTAYLINRFSTRKLFITSMSIFSIGCIIALFSNNFST
                     MLIARVLQAMGSGSLMPLLQVIILYLCPEEKRGAAMSLVGITVGFAPAIGPTLSGWLV
                     DSFGWHSLFLFLSPIAILDVILSFILLRNVGETQKLKLDIPSIVLSSLGFGGLLIGFT
                     NQGNYGWTNIATYLPILIGIMSLILFTLRQLKSKEPFLELRVFKNKPFLISTILIMIV
                     YASMMSATLMIPLYVQSVRGFSALSSGSLMLPGAILMVVLNPIAGRHLDKYGPHALSI
                     LGTGCLFLGTLSFAFLGRDTSLIHVSLMYCIRMIGISMVLMPLTTWGIKTLDRELISH
                     ATAINNTLRQISGAIGSAILITIMTSATKKAHMSSNMLSNIHGIDVAFSIAATLAFTG
                     LIVSICFIKRHQIIRS"
     gene            complement(9735..10643)
                     /locus_tag="PC471_000898"
     CDS             complement(9735..10643)
                     /locus_tag="PC471_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDF2788038.1"
                     /translation="MKGDNKVTEKQRLIITIAQKIFDQKGFQNTSISDVVKECKMSKA
                     TFYKHFETKESFICEIINYYDEKFLEIIHSINENNDIPSSEKLNRKIIAVWKNIFSRT
                     TINTYIRENFSEEQRKATSKLQTKSRANLLNEYKLSLFDNYGDKIEKIIFDLVFLLDA
                     LIHQFIYIIHVQKREINVYFIAKFTIQILDLVVENMDNLNPLIEKSMFLHKQEDEVYF
                     SLHNKSLFFKTIQDIEELIKTDTSLLENPKLLEAIKKLYVEGKNQQYDSLIMDAMIAY
                     LEKEDTLRPKVLLLNSIKNQLKKETL"
     gene            10904..11551
                     /locus_tag="PC471_000899"
     CDS             10904..11551
                     /locus_tag="PC471_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HDF2788039.1"
                     /translation="MKKKICVAIIFALFIVFVEMFIDSSSEGSKIMTAELSKYRKGVN
                     VLSINNIIVANKKYSLPKDYSPQESSEARDAFYKMNKDAQKSGLNLKAFSTYRSYEYQ
                     DRLFKSYVKEHGEKEANRFSAKPGESEHQTGLAFDIGGDDQSCWANKKFNNTKEAKWL
                     YENAYKYGFILRYPEGKENITGYMYESWHYRYVGTEHSKNFAMNNLTLEEYLHIN"
     gene            11876..14515
                     /locus_tag="PC471_000900"
     CDS             11876..14515
                     /locus_tag="PC471_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HDF2788040.1"
                     /translation="MSYKSTYQEVIKNLDSSLSGLSKERAEELLEKNGANELKEADKV
                     PTYKLFLESFKDPLVIILLIAALVQIFLGETVESIIIFAVIIINSVLGVVQTKKAESS
                     LESLKNLSAPNAKVIRDNKKMTIPAKNLVVGDIVFLEAGDYIPADGRLIEAQSLKVVE
                     GMLTGESEPVLKHIEKISEDVSLGDQKNMVFSGAVVVYGRGSFVVTGTGMNTEMGKIA
                     GLLESAENKLTPLQQKIEDFSKKLGVGIVILALLIFIIQVARNYFMAGNGEMTQIILD
                     SFMFSVAVAVAAIPEALSSIVTIVLAVGTNSMAKKQAIIRKLPAVETLGSTSVICTDK
                     TGTLTQNKMTVVDYYMYGTNNDELDKEKVNYSYHAKLLTMVSTLCNDSHITSDGKEVG
                     DPTEVALINYSSKNDLDYDKLRSKYIRINEIPFDSDRKLMSTVNSIYGDYIMFTKGAP
                     DIVFSRCKYALKNGSKVDITDEIIEEYKHKNEEFSNRALRVLAFAIKDVPEEDFIPSI
                     DDEHDMTLVGIMAMIDPPREEVMDAVKEAKSAGIKTVMITGDHKTTAAAIAKEIGIME
                     EGDLALTGKELDALSEEQLYEKLENISVYARVSPENKIRIVKAWQHKNNITAMTGDGV
                     NDAPALKQADIGIGMGSGTDVAKDASAMVLVDDNFASIVNAVEVGRTVYSNIIKSITY
                     LFAGNLGAIVSIVFAVFAGWSNPFTALQLLFINLVNDSLPAIALGFEKSEKNIMNKPP
                     RHPDESILAGGTLKAVAIRGCIIGVVTIIAQYIGLQVSPELGVAMAFSTLILARIMQT
                     LPARSNNQTVIKLGFFSNKYVIGAVLVCLGLYSLTLIPVLRGIFSIPTTFGLYQLGIC
                     LGLALISSILMELNKLINRSEVK"
     gene            complement(14573..15676)
                     /locus_tag="PC471_000901"
     CDS             complement(14573..15676)
                     /locus_tag="PC471_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate aminotransferase family
                     protein"
                     /protein_id="HDF2788041.1"
                     /translation="MKLKINKAIEMQLKKSYAIESGHSEDVFEIDCGEGINTVSYSNK
                     AVEAFNALKFDMIRGYPHSIALKDNIVDYWKDFIALDTDRICLADGSIHVIYLLNRLF
                     IEKGDKVLGYSPQFSEYETDIKMHGATYDYVLLKKEDNFKFNEKEFIEKINPEYKVIY
                     IDNPNNPTGQIIPLSSIENIVREAAKYDIAVMVDEAYGEYMPKENSAVKLLNNYDNVI
                     ALKTFSKGFGLAGLRAGYAVLPEQLVSPIKKISTPYEVSEISRSIAANLLDDVQFIEE
                     LKEKTKDIKNQLLIPWKNLNIAETSDTVSIMTVEHKNKDIDLQQEFAKLKIRVISGSD
                     FTGLDKNFIRFRMPEEKELPEVIKAFQIIDNIE"
     gene            16036..16857
                     /locus_tag="PC471_000902"
     CDS             16036..16857
                     /locus_tag="PC471_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDF2788042.1"
                     /translation="MGYKLIVTDMDGTLLGNNHKVTDENKTALQKVIKSGINVTLATG
                     RAFDSAKCNVDFLKEDMPIIACNGSLIREQNGNIIYSNKIDTRTCLNILDVLDKYDIY
                     YQCNSIDSMLSKKIEGREDRLSVFLGSETEVIVKDDLREEIFKKDILKFVIIEEKNPS
                     ILDEIRKELRKVQGIKITSSWPNNIEVMNEGVDKGNAVKILAEKMNIDREDIIAFGDN
                     YNDIEMIKFAGLGVAMGNAEELIKQEADYVTDTNQDSGVAKAIYKFLELKESLSS"
     misc_binding    16936..17147
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            17234..18550
                     /gene="argH"
                     /locus_tag="PC471_000903"
     CDS             17234..18550
                     /gene="argH"
                     /locus_tag="PC471_000903"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012101354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HDF2788043.1"
                     /translation="MKLWGGRFRKAENQLMEEFNKSFGYDCVLYKKDIEGSVAHVHMQ
                     VKCGLLTEEEGKSITEGLKGILEDVENGKLVLDGEYEDIHSFTEINLIQRIGDVGKKL
                     HTARSRNDQVAVDMRLYAKEKANDLVDLISEFKATIKDVADKNPVMMPGYTHLQRAQV
                     VTFKHHLMAYYSMFDRDEKRIKNAIEILDESPLGCGALAGTTHDIDRSITCEQLGFKK
                     VVDNFMDGVSDRDYLLELMSDFSIIMMHLSRLSEELILWSSQEFGFVEIDDLYTTGSS
                     IMPQKKNPDGAELIRGKTGRVYGNLFGLFTVMKGIPLAYNKDMQEDKEGFFDSVHTLE
                     MCIQIMDRMIATLKVNEDKMKQAVKNGFLNATEVADYLVKNNVAFRDAHGIVGSIVIY
                     CEDNKKAIEDLTLEELQKFSDAFKEDIYDFIDYESILNKGIKKNLK"
     gene            18799..19137
                     /locus_tag="PC471_000904"
     CDS             18799..19137
                     /locus_tag="PC471_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2325 domain-containing protein"
                     /protein_id="HDF2788044.1"
                     /translation="MNLLVIGGHERMEKDYMVMAKKKGYKTKVYTTMSSKLNNSIGKP
                     DAVVILTSTVSHKMSRTVESQAKKQDILIVRHKNSSKVAFNECLDMVDECLGNCSKCK
                     LKDKQKQKNI"
     gene            complement(19264..20553)
                     /locus_tag="PC471_000905"
     CDS             complement(19264..20553)
                     /locus_tag="PC471_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HDF2788045.1"
                     /translation="MKRNLSLLLICLLIFTSFLGSSNISFADNEPAIVAKHAVLMDYE
                     SGKILYNKDGNSKLYPASTTKVWTACLVLKEVKDLNQVIEIKDLPQIDGSSMYLKNGE
                     SFTVKQLLDALLVHSANDAAFVLARYVGGGNVQKFIDLMNSEAKKIGATNTHFNNPHG
                     LPDPNHYTTAHDMALIAREAMNNDTFRQIVKTKSLKFEATKAYPYERYFVNTNKFLTS
                     HDKITYKGQPINIKYDIVDGIKTGYTDAAGKCLLSSAVKDGRRVIVAVFNSTNADLYL
                     DSRILIDYGFDNFKCATIVDKEKYTDTKKVLFTKQHELIYEPKNSYRIFLEKDESQGN
                     YDTKTELNKIDLPIKKGAKVGTLNVYNNGKLENSIDLIAKNNLDSSLPFLTENNVLMT
                     FVKIIAGILVLLVLFIIASNIKKKKRKKARSKKNMKK"
     gene            20633..21337
                     /locus_tag="PC471_000906"
     CDS             20633..21337
                     /locus_tag="PC471_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HDF2788046.1"
                     /translation="MKKKVKFISFFVLFILIISSIIVYKSLIFKDDIYVVSKKNLPNE
                     KTHVVENEIKGEDNNHSKVTKDIDKNFQNNKITIYISGAVNRPGIVTIESDKRLYDAV
                     ELLGGTTKEADLNGVNLSVRLEDEQHYIIPKIGEATSVTSNDDSNKLNQKNESRGEPK
                     NKSNKNISNESKVNINVATIEELDSLPGVGEATANKILQHREENGQFSSIEEIKNVNG
                     IGDKKYENIKDLICVD"
     gene            21429..22475
                     /gene="selD"
                     /locus_tag="PC471_000907"
     CDS             21429..22475
                     /gene="selD"
                     /locus_tag="PC471_000907"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015617929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:21477..21479,aa:Sec)
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HDF2788047.1"
                     /translation="MKKEQKKLTEMTTAGGUAAKIGPEVLASVLSQLPKNDNIENLLV
                     GLDTADDAAVYKLNDDMALIQTLDFFTPMVDDPYVFGQIAASNSLSDVYAMGGKPLVA
                     MNIVCFPSCHDMDVLAEILKGGFDKVKESGALLVGGHTVDDKEPKYGLSVSGIVSPNK
                     VLSNATAKPGDKLIITKPIGVGVLNTAMKEGMVEQHIADKVIEIMIHLNKYAAMSFEK
                     FDVNSVTDITGFGLLGHTLEMAKASEVSIEIESKHVPIIEGAIEMAQMGIIPAGMYKN
                     MHYVSKDVEVVGNIEVAVQDILYDPQTSGGLLISVKEELAEELVKDMKLNGAIEAKII
                     GSVVPKGEKYITVL"
     gene            22493..23905
                     /locus_tag="PC471_000908"
     CDS             22493..23905
                     /locus_tag="PC471_000908"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HDF2788048.1"
                     /translation="MIVLSKRELFAMLPSVDEVLSDNRIVEIINEYPRSLVLESVREV
                     IDLKRQFILRLKEDASNSVTIEFGEIIESAIERVKLNYSLSLKKVINATGTVIHTNLG
                     RSLLSEDIKDELWCAASRYSNLEYDLDNGERGSRYSHLTSTIKRLTGAEDVLVVNNNA
                     AAVLLVLSTMAKGGEAIVSRGELVEVGGSFRIPSIMALSGAELVEVGSTNKTHLKDYK
                     EAITEDTNVLMKVHTSNYRIMGFTESVSIEELVNLGKKYKLPVIEDLGSGVFIDLSKY
                     GLSYEPTVLDSIRQGADVVTFSGDKMLGGPQAGIIVGKKEYIEKMKKNQLTRALRVDK
                     LTICALEATLRMYLDETKAIENIPTLKMLTYKIEELEVKANKLFEKITALNLNANINI
                     EDGFSQVGGGSMPLETISTKVISITPEHMNVSSLEKKLRLSEAHIIARVYDNKYVLDV
                     RTIFDDEFDVIVEELRKAFN"
     gene            24019..25902
                     /gene="selB"
                     /locus_tag="PC471_000909"
     CDS             24019..25902
                     /gene="selB"
                     /locus_tag="PC471_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HDF2788049.1"
                     /translation="MKNVVIGTAGHIDHGKTTLIKALTGRETDTLDEEKKRGISINLG
                     FTYFDLPNNRRAGIVDVPGHEKFIKNMLAGASGIDIVLLVVAADEGVMPQTVEHLDIL
                     SFLNIKNGIVVLTKSDTVDDEFRELVKEDICEKIKGTFLEDAEIIEVDSISKRGIDTL
                     IEKIDKMSDDIEDKNENSPARLNIDRVFSIKGFGTVITGTLIEGKISIEDDLVIYPQV
                     LKTKIRSIQVHGENKDTAYAGQRTAINISNVKVDDIKRGDVLAAPNSLEESMMLDVKL
                     SLVNHLDKGLKHWDRLRLYHGTREILCRAVPLDKELIESGESGYVQLRLEESIVSKKG
                     DTFVVRRYSPMETIGGGVIIDPSPKKHKKFDEKVIEALKIKEKGELKDIIEEYLKRNL
                     KNYPNIKEIMSYSGAHEEDVKRALETLISEDKVFIIGNMYMHINQYNKLKENTIKLLS
                     EYHKKYRLRKGILKEEVRSKIESNFKTREMDILLEKLSTENAIKIENNIVSLLDFEVI
                     LNDKQKEIAKKIEKRLKSCGVSSILTIDEVSEGNHNYAEVLESMIGNKVEKLDDLYIM
                     DKDIYENAKNILINYIKENKEITLGEYRDLIDSSRKNCMIILENFDRNKITKRVENKR
                     ILF"
     gene            26007..28745
                     /locus_tag="PC471_000910"
     CDS             26007..28745
                     /locus_tag="PC471_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation-associated two-component system
                     sensor histidine kinase"
                     /protein_id="HDF2788050.1"
                     /translation="MKNIIPDVKPDIKEREDMSLLFIIMGSVLLLFIIKDIEKYETLL
                     HAIYTIFLVATGVIIFNTTKFKVDSFSTFLGLIFIATGVLECIYLFNSLGIKTKSIME
                     INITISAITDLFPILGVYLSFKFVKDNKQIYSSVILFIATATLTITSLFIISGICDYL
                     GNGKSQYALGVIISIFIIIISIISGIELNSSSNKGKWEYDEKKIFNRIIVIIILSRVP
                     NLLHIVIDNRNVERILSQIIINVALYYLYNYIVSKNIKKTALELHDTNEELTKKTESL
                     KEKNKKLIYETEKIEELKGILMKRESRLQSTLDVAVNSIVVFSKDGEITYANKSFRNT
                     FGDYKEQDKLTDKIKNFNDLIESIHYVFVHEKNAEKLISTSDNKVYQAIFAPLIIASQ
                     TEGVLCVLEDKTKKKEYERKLIEANKRSKDFLESIGDGIVVLEGNNKIYVNNACREIF
                     KNNLESIDFSLVCKSEESIEKRYVIDGEVKYVEMSFSQYTNAGTNKTIIVIRDTTSRK
                     ISQIKLEESQSSYSRFIDILPDGICLLKEDLSINYANKSFLDMLAFTNIDDIKDSNIK
                     LLMNANTEEKMKFTDNMAKVLNENRSILLLEHELINSYDDIVEVELSALPFAIYNTRY
                     IMLIIKDLVHKKYSEQAEKELLERFKTDKIKTEFFANMSHELKTPLNVISSSNQLVDS
                     FYRNEKIKDYNNNIKSHVDLVRQSSYRLQRLINNIIDLTKMESGFYTLKLAKYNIVSV
                     IEDLFMNIEEYALRKDIKILFDTDLEEINVYIDKVEIERIMLNLLSNCIKFTDNGGWI
                     YVSIHYKIDKVIISVKDTGVGIPQDKLELIFEEFSQVDKTLSRNTEGSGIGLAIVKNL
                     VSLHGGDIEVVSEVNKGTEFLISLPMKGFSSEHYTEDKRIYNIQEKIKIEFSDIYY"
     gene            complement(28864..29808)
                     /gene="manA"
                     /locus_tag="PC471_000911"
     CDS             complement(28864..29808)
                     /gene="manA"
                     /locus_tag="PC471_000911"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HDF2788051.1"
                     /translation="MEPLFLKPIFMDRIWGGTALKDKFNYEIDSPTTGECWAISSHKN
                     GDCLIENGKYKGKKLSELWNKNRELFGNTPGDKFPLLTKILDANDNLSVQVHPNDEYA
                     KKNENGELGKTECWYVIDCSDDAEIIIGHNAKSHKEFVNMVNNNEWDKLLRKVNIKKG
                     DFFYVPSGTIHAICKGTLILETQQNSDTTYRVYDYDRTDNSGNKRELHVQKSIDVTNV
                     PHINFDTDYKIVSTSDFKCTTFVSNEFFSVYKLDVFGKCNFTHNTPFSLYSVLDGNGK
                     LIHNSVEYNLKKGMHFILPNDFGDFSFEGNLEIICSHI"
     gene            29981..31342
                     /locus_tag="PC471_000912"
     CDS             29981..31342
                     /locus_tag="PC471_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HDF2788052.1"
                     /translation="MELFKAESKKYKKEFLKLLEQWVSIPSFYDRKTVSKDMPFGKGV
                     YDALNWFENLGRENNFKVKNIDNHAVQIEYGNGKEYVDIFGHCDVVNPGEGWDSEPFK
                     LNIIGDKLVARGVSDNKGPMIVNFLALKMIKDLDINLKRKVRLIAGGNEESGFKCIKH
                     YYSKEPYGVCGFTPDAKFPVLNGEKGGAIIKLISNIDDKSLYISGGIEFNTIPDKVYI
                     KNVEKLGKDNICFDINNISINYDNGNYIVYGKGGHSSKPEKSINPILATIKLLSENID
                     EKWTKDLYKLINQDNINGNLFGLNIEGKCGILSMVPTIINIVDGKLEVVLSVRYPEIL
                     TIEDIIKKFNLYMEQNNINKFELIGENLKQANYIDRNSKLVRSLHDIYIKYSGDLKND
                     VRVTSAGSYASEMNNSVIFGCEFPDGSFGNVHSANEFASLDRFITAIGIYAEAIITLC
                     NKI"
     gene            31430..33319
                     /locus_tag="PC471_000913"
     CDS             31430..33319
                     /locus_tag="PC471_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HDF2788053.1"
                     /translation="MITKKHAIIVKSLSNKDGYMTSNELAIKLDVSTKTIKRYIADLN
                     SVLSKYDLVIASSRGIGYKLSGSKNNIARAVKEANKYIDGFLDDSEESRMSNIICMLI
                     NRNYMSIEAMAEELNLSIAAINKLSSKLKRKLEKYDLVIKSKPYYGSYIHGEEINIRQ
                     LITDYAIKLDEKSKVKVFLDDISENDIHCIESILEKHLREKDTIISDKDFNLLLSKII
                     VSVFRSKRGHSNNINLMDTSYRFHNYYFIENLMKEISDKIGFKLIEDEVIYISNYSGV
                     IAYKGTQGRKNTSEIEERISSVISSALQDIFLISGSDYTKDDEFMVAISDHIKRFLNR
                     ARANVKSNNPLLHQIKEKFPIAFNLAVFLSNKLETEFNLKLDEDELGYIAIHFAASNE
                     RMKKNTSKKICIVCHYGIGTGQLISEKLKQNISDLSVVGVYPVRYLDMAISQDVDLIV
                     STVELKGYEKPVLYIENIFDDSLIENVNKAFYEKEERRKIISNMFDEKAFFSIKANTK
                     EEVIISLSNKLKERDFIEESSIKSIIDRENISSTEIGNLVAIPHTIVKGDKKSIIGVG
                     ILENPIIWDKQEVQLVFMVFFNTKEKHNFSIFKYLYNFIKDEGGVRGIIKICDFNKLM
                     ALIGN"
     gene            33334..33789
                     /locus_tag="PC471_000914"
     CDS             33334..33789
                     /locus_tag="PC471_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDF2788054.1"
                     /translation="MGTNIFNKEYVFLNVDAKSKVEVLKFISKKAKDLNLAEDESLVY
                     EGLMAREEQFTTNLGESIAIPHTKNDAIENPAVVVLKFNEDVVWNEGEDKVKLAISLL
                     MPGKSKENIHLKLLSSLSRKLINKEFKDSLLKSDNVEEISNLINEALGL"
     gene            33848..34165
                     /locus_tag="PC471_000915"
     CDS             33848..34165
                     /locus_tag="PC471_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HDF2788055.1"
                     /translation="MNKKLVALCACPMGLAHTFMAAEAIEQAAKALGYEAKVETQGAD
                     GVQNELTRDDILGATMIIHAVAITPEGMERFDGCEVYEVELQEAIKNAEGVIKEIEED
                     LGI"
     gene            34190..35452
                     /locus_tag="PC471_000916"
     CDS             34190..35452
                     /locus_tag="PC471_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HDF2788056.1"
                     /translation="MAIKKKVINSSGASTTGGNNVGKSTPSSPTQSKGNGKWKEISKH
                     IMTGISYMIPVLVMGGLIGALSQLIPYAILGLDPSVGIVDAMNSGEFTGFKLSLLNIA
                     QLMSNFGFTLFGFAIPLFAAFCANSIGGKTALIAGFIGGYIANKPVGVPQFVDGQWTE
                     VVPVASGFLGAILIAFIIGYFVKWLNKSIKVSHNWLAFKTTFLIPLIASLACMVLMIF
                     IITPFGGLINESMKNFLTAAGAAGEYVYATALAAATAFDLGGPINKAAGFVALGLTTE
                     NVLPITARTIAIVVPPIGLGLTTLLDKRLVGRRVYDRQFYQAGKTSIFLSFMGISEGA
                     IPFALERPGFVIPLNIVGSVIGAITGIILGAIQWFPESAIWAWPLVDNLFGYIIGIAV
                     GAIFIAVGNIFYRNKLIKDGKLVVDYID"
     gene            35557..36621
                     /locus_tag="PC471_000917"
     CDS             35557..36621
                     /locus_tag="PC471_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HDF2788057.1"
                     /translation="MDKIEKVREYFREYDIDGFLINSPTNKFYVGNLFSSSGYVFITK
                     ESQYIIVDFRYFEEIKRKSSLFNVVLMDKTRTHFDIINDICREQNIKEIGFEGNEVSF
                     DLYRSMSNKLSATLKSVDLSTLRETKNEDEIKYIKKACEIVDATFYHIVDFIKVGMTE
                     KQVENEIVRIIKDLGGQKESFDTIVASGLRGALPHGKASEKVIEYGDFVTFDFGAKYN
                     NYCSDITRTICMGTINKELEEIYNIVRKANEECIRVLRPGMTTGEIDKVARDIIGSYG
                     YAGNFGHNLGHGVGIMVHEYPALAPESNEVLKEGMIVTIEPGIYVPSLGGVRIEDDVL
                     ITQDGCMRLTTSTKDLIVVS"
     gene            36675..37853
                     /locus_tag="PC471_000918"
     CDS             36675..37853
                     /locus_tag="PC471_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PatB family C-S lyase"
                     /protein_id="HDF2788058.1"
                     /translation="MYNFDDKPDRVSEKCRKWDLNIIRDKFGDIREDFIPMWIADMDF
                     KIPTEIENKFIEAVRRGVFGYTYCYDEFYDSVIGWQKNMHEVNVEKEWITLTYGTVST
                     LHYVVQAFCKKGDSIILNTPVYDPFESSAKKQGVNVICNTLDVVNDRYYINFDKLEVQ
                     IKENKPKLMMFCTPHNPSGRIWTIEEMTRVATICKENNVILVADEVHAEHIHYGTFNS
                     ILKIGKELLENVILLTSPNKGFNLGGLKTSYSIVINKDIRDKFRNKLKQNSITSPNVF
                     GIIGLITAYNECHEWLSGVNEYIKSNYELLETWVNKYNKIKLMKMESSYLAWMDISGL
                     GISASEFTDKLAIDTGVLLEDGSHFVKDGEKYVRINLGTQKENVIEALNRMDLFLKSL
                     "
     gene            complement(38044..39102)
                     /locus_tag="PC471_000919"
     CDS             complement(38044..39102)
                     /locus_tag="PC471_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HDF2788059.1"
                     /translation="MNKKKITFIFVILILALILFSFFRSSSQNTKYSKTSYYLGTVNE
                     VTVFNVKESKSDKILNECDSILRYIDNKMSTHIPGSDVSKINDNAGKKFVRVSDDTFF
                     VVKEAIQYSKLSDGYFDITIGPLSNLWAIGTDKAKVPSDSEIQKLLPLIDYKNIILDE
                     KNKSIKLTKENMKIDLGAIAKGYAADKIVAYLKSEDVKKGLVNLGGNIFTLENGKNDK
                     PFKIGIQDPTSKNGESIGNIETTNKSVVTSGIYERFIEKNGKIYHHMLNPFTGYPFEN
                     NLSSVTIISDKSINCDALSTSTFGLGLEKGMNLINKIDNVDAIFITKDKKVYLTKGIK
                     DNFKLTDKSFSIEKLNKQ"
     gene            39169..39540
                     /locus_tag="PC471_000920"
     CDS             39169..39540
                     /locus_tag="PC471_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HDF2788060.1"
                     /translation="MKKKDILLILGILVVITACYGIINVINSKNAGNIEIYVDNKLYK
                     TVSINAKEEFKIENRGGYNIVKIHDKGVEIVDASCPDKVCVHTGFINKPSQSIVCIPN
                     RVSIKINTNEKNDNQEDIISK"
     gene            39577..40251
                     /locus_tag="PC471_000921"
     CDS             39577..40251
                     /locus_tag="PC471_000921"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HDF2788061.1"
                     /translation="MVNLGNDWDELLKEEFEKDYYLNLRKFLIEEYKTRQIFPNMHNI
                     YEALKHTSYKDTKVLILGQDPYHGDNQAHGLAFSVQPQVKTPPSLLNMYKELKDDLGC
                     FIPNNGYLMPWADQGVLLLNTALTVRAHEANSHKNKGWEIFTDRVISILSEREDPVIF
                     VLWGSNARKKVELIDTSKHYILEAPHPSPLSASKGFFGCKHFSKINEILKKLGKEPIN
                     WQIENI"
     gene            40500..41339
                     /locus_tag="PC471_000922"
     CDS             40500..41339
                     /locus_tag="PC471_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HDF2788062.1"
                     /translation="MNFKKLSNAKVSVMAVVLSVGILSGYSALNKQVTLVVRGEERTI
                     STFSSNVGDLLESQNISYDANDIVTLDENTKLSNGDKIEVIDVKEKTVVETKDIPFEV
                     TVVEDGSLLKGDSKVKEEGKSGKSELIYKETYHNGKKVEKEFVKKVVKAEPVNKVVSK
                     GTKVEMQVASSRGESSRRMASSSSSSSSSNNGKNMRVVATAYTGHSITATGTKPKWGT
                     IAVDPKVIPYGTKVYIPQFDMVFTAEDCGGAIKGNKIDIYMNDSKTVYNWGRKTIDIR
                     ILK"
     gene            41583..41679
                     /locus_tag="PC471_000923"
     tRNA            41583..41679
                     /locus_tag="PC471_000923"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:41617..41619,aa:Sec,seq:tca)
     gene            41829..42536
                     /locus_tag="PC471_000924"
     CDS             41829..42536
                     /locus_tag="PC471_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA threonylcarbamoyladenosine dehydratase"
                     /protein_id="HDF2788063.1"
                     /translation="MENNFTTRTSFLVGDDGIEKLNNSNIIVFGVGGVGSFTVEALAR
                     AGVGNITIVDFDDVDITNINRQIPALHSTVGRYKVDVMEERILDINPNINIKKIRSLY
                     NKDTSDEILTERYDYVVDAIDMVSSKIHLIETCEKKGLKIISSMGMGNKLDPTKIVVT
                     DIHKTSTCPLAKVMRKELRDRGIKKLKVVYSTEQPIELKKKVMNGRKVTPGSVSFVPS
                     VGGLIIASVIVNELLGQ"
     gene            complement(42678..43514)
                     /locus_tag="PC471_000925"
     CDS             complement(42678..43514)
                     /locus_tag="PC471_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDF2788064.1"
                     /translation="MRNKIFLSILIIISLLIGCDKKSLLSIHMIDVGQGDSILVQTPT
                     NKNILIDGGDEDSENIIISYLRQKRIKTIDIIIATHPDSDHIGSLDNIIKKFNVNSIY
                     MPEQSTDSEAYQNLINSCTDKNLSIQHLYKNDVLNIDNNINIYVLSPSYIQEESNLNS
                     IVFKLTFNDNSFLFMGDAEEENEKEILHSFKLNNINFIKIGHHGSNSSSSLEFIKKIS
                     PDIAAISCGYKNQYGHPHREVINNLKQNHVSIYRTDRIGDIVFYSDGEIIFTKYNYEI
                     DT"
     gene            43702..44607
                     /locus_tag="PC471_000926"
     CDS             43702..44607
                     /locus_tag="PC471_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2156 domain-containing protein"
                     /protein_id="HDF2788065.1"
                     /translation="MFFKDIELNSKKELDPYFDLVDYEACEYCFSTLYMWQHVYKTGY
                     YIGEDFAVLVGEYEGDSFSILPLAKKDKLPEVVDFVLEYFSKNNKKIYLRGITTEVVE
                     FLKEKYPGRFEYIEERDLFDYIYDAESLRTLAGKKNQKKRNHINYFLKEYAGRYEAKL
                     LDKENFDECLVLMKEWESNKEENNEFDESMDDELIGIKKIFNHYDILKDKVKVFGVYV
                     DGKLEAFSIGELLNPNMALIHIEKANPDIRGLYPFINQQFLVSEFKDVEFVNREEDLG
                     IEGLRKAKLSYHPCRFVEKYSVREA"
     gene            44616..45800
                     /locus_tag="PC471_000927"
     CDS             44616..45800
                     /locus_tag="PC471_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDF2788066.1"
                     /translation="MEIRYAKEEEIESIKEIWSYCFNDTESFMKYYFNDKYKSENTVV
                     ALDEGKIISSLQLNQYKLLLNSKVYNTSYVVGVSTLPEGRGAGYMNKVMKFTLNELYK
                     KGQLVSILMPIDYRLYRRFGYEHCYDQIEYTINTDDLKNFKSSGKMIKSNLSQIDDLI
                     QIDRTFLNEVNGNVLKDEHYYENLFKEIQSEDGFLYIHEGNEKDGYIVYFLQEDKMFV
                     RELFYKNIDALKSMLKFIYNHNTQCKIVTISTPTIDKIRFILDNPKDSDIKIKPFMMG
                     RVINVKKFIEDIDIENDINSSFNLLIEDKFIDENNGLFKISIQNKKVSVEQLDKKGAE
                     KPQEDFDIKLDINTLTQLSFSYIDVNEAIFLNDIKDVSEETLETLNCIFSKKNNYINE
                     YI"
     gene            45915..47216
                     /locus_tag="PC471_000928"
     CDS             45915..47216
                     /locus_tag="PC471_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HDF2788067.1"
                     /translation="MGVIIIQIRRPLLIILMFVVAISFIITNNTKNDEELNDKIITIQ
                     GVVKGKIEKKRYNQYKVGVFLINDYTKNKNLKIGQKVNITGKFKSLDKMKYDDFDYGR
                     YIKSTGYKGIVYINNYKIIDKNKLYSLIGEIKFYISKTYRYLYKKNSDFINSILLAEV
                     ENLTEEQKEIFTRTGTSHVISISGLHTGILCVIISFLLRGINKLYKLLILSIFITLYC
                     IMVGASPSIIRSIAFVMIFYLSIFIDRKKDGISALSLIGIILIMNNPYVIYNVSFQLS
                     FLATLSILIFYNKINSIIKLSMVSLTISSNILTLPIIYYTFKGIPLLSIIGNLIIVPF
                     VGVIMYLSIASLIVFKVSVVIAKIISFFNSTFIESIFFLLEKISNLSFAYINIENPKF
                     YIVVIYYIGVFFYIFYIEGKEIKEQENELQGYYKECKREKL"
     gene            47170..48213
                     /gene="holA"
                     /locus_tag="PC471_000929"
     CDS             47170..48213
                     /gene="holA"
                     /locus_tag="PC471_000929"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HDF2788068.1"
                     /translation="MNYKDIIRNVKEKNFEKMYLFYGREYYLIENAIKAFKDSLNEGM
                     LDFNLDIIDGKEIVLNQLISSIETLPFMDDRKIVIIKDFELLKGKKKNFTDSDEKYLI
                     EHLDNIPDTTTIVFVVYGDVDKRKSLVKKIGNNGIVFDCDKLSDMDLFKWIKKSFALN
                     DVIIDNSQIMYFIEQEGYRDKSSEKTLSDLENEINKISSFVGKGNNVTNDVIDKLSQK
                     KVENDIFKLIDYIGEQNASNAMKILNDMIQEGESVLGIFSMIARQFKIIMQVRQLQLD
                     GYSTKLIADKLKMHQFVVGKALKQTKNFSDDIIVEILNYILESDYKIKTGLIRDTLAV
                     EMLVSRYCKREAI"
     gene            complement(48400..48666)
                     /gene="rpsT"
                     /locus_tag="PC471_000930"
     CDS             complement(48400..48666)
                     /gene="rpsT"
                     /locus_tag="PC471_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HDF2788069.1"
                     /translation="MANIKSAKKRISVIEKKTALNRVRKSQIKTAIRRFEDAVAAGNR
                     EDAVAKFQYAQKRIYQVASKGTIHKNAAARKVAKLAQKLNGMNA"
BASE COUNT        19143 a         5266 c         8351 g        16032 t
ORIGIN      
        1 tgtaaaaaat atttttaaat tatcatttaa tgtaatttaa atgaattgaa gatatatgtt
       61 tattgtatgc aattgttgta tttttaggtt ttttgtgatt aattaaaaaa ttataaaaat
      121 cgtttacatg ttgattttta aaaaaaaaag atatagaatt atatagaaag taataaacaa
      181 aaattggatt tacgggagtg gaatgaaatg atgaatgtta ccttaacaga tgtgaaagag
      241 gctagagaga ccattaagaa catagtaaaa agaactgact tacttgaaag tgttaaactt
      301 agcgaaaaaa caggagcaaa tgtgttctat aaatgtgaga atcttcaaaa aacaggttct
      361 tttaaattaa gaggagcttg taacaaaata gcgagtttaa cagatgaaga aaaggcaaat
      421 ggagttatag cttcaagtgc agggaatcat gctcaaggag ttgcattggg agcaaaaatg
      481 actggtataa aatctacaat agtaatgcca gcaactgcgc ctttagcaaa agttagtgca
      541 actaaaggtt atggagcaga agtagtttta aatggtgcgg tttatgatga tgcatatgca
      601 aaagctgttg aaatacaaaa agaaacagga gcaactttct tacatccatt caatgataag
      661 tatgttatag caggacaagg aactatatct cttgaaattt tcgaacaatt agacaataaa
      721 gttgatacta tactatgccc agttggtgga ggaggaatca tctctggtgt tgctgttgca
      781 gcaaaagcat taaatcctaa tgtaaaaata gtcggtgttc aaactgcaaa tataccttca
      841 atgaaagagt caataaaaaa tggaaaagta acaacagcat ttaatgatac tacaatagct
      901 gatggtatag ctgttaaaac tccaggagat ttaacttttg aaataataaa tgaattggtt
      961 gatgaaatag tagtggttga agagacagaa attgcagaat caatattatt tatgatggaa
     1021 agtcaaaaaa tagtttcaga aggtgctggt gctgtatgta cagctgctat attaagtggt
     1081 aagtatgtac cagcaaaaga tgaaaatgta gtttgtataa tatctggtgg taatatagac
     1141 ataaatacgt tatatagaat aataggtgtt gctttagcta aagaaggaag aagatattca
     1201 tttagtacaa ttatggaaga taagccagga aactttgctg agttaactag aataataagt
     1261 gagaatggtg gaaacatttt aagtgctaat caaggtaaat tatcagcagg ggaagctcta
     1321 ggaaaacaaa gtgcggaatt tatattagag acaatagatt atgaccatat agctagaata
     1381 aagaaagcta tagaagaaaa aggttttaaa attatagaat tataataaaa agtgtatata
     1441 atatacaaga aaaatagtat aatagtgtat gaagatacat tttatgtgta ttagattaac
     1501 gaattaggag gattttaaaa tgaaacatga agtaatacat actaatgatg caccagcagc
     1561 tttaggacca tattctcaag ctataaaagc tggaaacttg ttatttgtat ctggacaagt
     1621 tcctcttgta ccagagacta tggaagtagt tgaaggagat gtacaagctc aaactgctca
     1681 atctttaaaa aatcttaaag ctatattagc tgaaagtgga gctgattttt caaatgtagt
     1741 taagactaca gtatttataa aagatatgaa tgaatttggg gctataaacg aagtttatgc
     1801 tgaatatttt ggagaaaata aaccagcaag agcttgtgta gaagttgcta gattaccaaa
     1861 agacgttaaa gttgaaatag aagtaatagc tgtattataa aaatacaata tttttaattt
     1921 gaaaaggctg aaaaactata ttctagtgat tcagcctttt tataaaaaaa taaatcacaa
     1981 taaagaacat gataaatatt gtcgaattaa aaaataaaat taaaataaat attgcaatat
     2041 ttttgagaag gttttataat gagtatataa tttgaaacgt gtaactatta aattaggcat
     2101 gagttcttgg atatttttat cctgaggatt catgcctttt ttgtattgaa attcaaaaat
     2161 ctatttaata aggaggaagt actgtgttaa attttttcaa gaaaaataaa agctataagt
     2221 tacatgcagt agtgagtggt aacagcatta atattgagaa agtaaacgat tctgtgtttt
     2281 caaaaaagtt aatgggagat ggagttgcaa ttattcctaa tagtaatgtt gttgtagccc
     2341 cttgtaatgg taaagtaact gtgcttacag aatcaaagca tgcctttggt atggtctcag
     2401 atgagggtgt tgaaatatta gtccacatag gaattgatac agttagttta cagggtgagg
     2461 ggtttaaaaa tgaagtgtct caaggagata ctgttaaaaa aggtagtcca ataataagtt
     2521 ttgaaagaga aaaaataaat tctcagggaa ttgattgtac aacaataata attgtattaa
     2581 atcatagcga attttcagaa ataaattgca tggtagaaaa tgaagttgtt gcagggcaag
     2641 atactgttat tgaaattatg aaataatagg aagggattaa ttcaatatga gagtagtaaa
     2701 aatctttaat aataatgcat tatctacagt aactaatgat aggaaagaag ctattttact
     2761 aggtcttggt ataggtttta ataaacgtcc aggagataaa gttaatgaag ataaaataga
     2821 aaagatttat tatgttcaag accacatgca aacaaaattt ttagaattat taaaaaatgt
     2881 tagtccagaa gtgatggatg cttcacaaca aattataagt cttataccag gaaatgaggg
     2941 aaaatttaat aataaaggga tattatcttt agtggaacat attagttttg caatagatag
     3001 aatgaaaaat aatgtttttt tacctaattt aatgcttttg gatataaaga tgatgtattc
     3061 aaaagagttt gaattaggag taaaagcttt agaaattata tatagagtat gtcatatcca
     3121 tttgcctgaa gatgaagcag gttacatagc tttacatttt gtcaatttac aatcaaatga
     3181 taatttggca tatgatactt taaaatttgt aaaaggtagt attgatttga ttaaagaatg
     3241 ttatggatta gaattagacg aatcgagttt aagtacatta cgttttagaa ctcatcttaa
     3301 atttcttgct caaagaattt ttaagaatga aatttgtcag gatgataaaa tgattgaaat
     3361 gtatgattat ttaattaaca atcatcctaa aaataaagaa tatttagaaa agttaaatgt
     3421 atatattgaa aaggagttta ggtataaatt agataaacct gaaaaaattt atctactact
     3481 acacctaaca aaaatcttat aagaatgaga tgaattagta atagaaatat ataattttag
     3541 ggggggataa gattgaaaga aaaactactt ggaggactgg aaacatttgc aaaggcaatg
     3601 gttcagccat taatgtattt atcgagtgca ggaatattaa tggtggtagg tgtactactt
     3661 acaaatgata tgttattagg agtacttcca ttttttaaat gggagccaat tcaaatattt
     3721 ggaagcctaa tttataactg tataatggct attattaata atcttgctgt tatatttgct
     3781 ataggtattc ctgctgcact agcaaaaaaa gagaagcaca aagcagcgtt aataggattt
     3841 atgagttata taatatacct tacagcttcc aacactatgt taacatcact aggtcaacta
     3901 gcaaaagaaa gcaagatgct tggactgata gggactggac aagctactat tttaggtatt
     3961 caagttgtag atacaagtgt atttggtgga attattttag ggtgtatagc tggatatgta
     4021 tttaatcgta caagcaacaa aacatttaaa ggagctttac agatatactc gggagcaaat
     4081 tttagtttta tatgcatgtt ctttgtatcc attttaattg gagttgtgag taactttata
     4141 tggccattag ctcaacaagg aatagcttct cttgcaaatg tcatcaaaga tacaggtaca
     4201 tttggtttat tcttatatgg ttttttagag agaatattag taccaactgg acttcatcat
     4261 ttaatctata caccatttca gttttctgat ttaggaggag tgcttaaagt tggtgcagat
     4321 acagtttcag gagcctatgc tatagttatg acagaaatga atttaccagt tgagcgtttc
     4381 tcagatagta tatactacat ggcaactgga tttacaaaga tgtttggtta tataggtata
     4441 ggagctgcct ttatttatac tgcatataag caaaataaga agaaagttaa ggcaacgatt
     4501 attccactaa taattacggc agtattagcc tctgtaacag aaccattaga ctttatgttt
     4561 gtattctgtg caccattttt attctttgta catgcagtta tagctggtct ttttatagct
     4621 ttattaaaaa tatttgatgt tactgcatta tgtggaggga acttattagt atcaattata
     4681 atgaatacta cagtaggcgt agaaaaaaca cactggccag taatgatgat attaggactt
     4741 gcacaaatta ttgtatattt tgtagtattt agttttttaa ttaagaaatt taattataaa
     4801 actccaggtc gtgaagatga gagtacagct acaggcgaag aacaatcaaa acaacttaat
     4861 ttagatgcag ggatagaaaa tataattgat gggttaggcg gaaaagaaaa tattaataca
     4921 gtagaaaatt gcatcactag attacgtgtt aatgtaaaag atgaaagtaa gattaatgaa
     4981 gatataatta atttaacgcc aaatagcggg attgtaagga aaggaaaaga tatacaaata
     5041 atttttggac ttcatgtgca tgaggtaaga cgtgcagtag aagatttttt agaagcttat
     5101 taaagatata tatttatata gcaagggtaa ataacagctt taagtgctgt ttactattta
     5161 ttaaagatac atataattta tagaggagga ataattatta tgattattac tattgttggt
     5221 ggaggaagca catttactcc aggtattgtt aagtcaattg cattaagaga aaaagagtta
     5281 gagcttgagg aaattcgctt atttgacatt gataaagaac gccaagataa agtagctgta
     5341 gttgtcaact ggatactaaa agaagatgtt aaaacagatg ttaaactaac tgtaactaat
     5401 tgtgaaaaag aagcgtatac agatgctaca tttatatttg cacaaatgcg tgttggtaaa
     5461 tatgaaatgc gtgagcagga tgaaaaaatt ccattgaaat atggttgtgt aggtcaagaa
     5521 acttgtggtt gtggagggat ggcatacgga ctgcgtacaa tacaaccaat gataaaggtt
     5581 attgatgatg tagagaaata tgctaaaaaa gattattgga tattaaatta ctctaatcct
     5641 gctgcaatcg tttctgaagc ttgtcgtaag ttaagaccaa atgcaaggat tataaatatc
     5701 tgtgatatgc caatagctat tatagatatg atagcggggt cattaaatat caatgacgtt
     5761 cataatatac gctatgatta ttttggtcta aatcattttg gctggtttac ttcaatagat
     5821 tataaacata gagacttaat ggaagagatt aaggaataca taaaagaaaa taaaatatta
     5881 ttaccaaaat cttatataga aaacttaaat aaaagtcgta gtacaggaaa tcgccatgct
     5941 gtaagtagct ggatacatgt ttgggaatct gtttatacaa tgttagaaag cttcccaaaa
     6001 tatctgccaa acacttattt aacatactat cttttaagta aagaaacagt ggaacattca
     6061 aacataaatc gtactcgtgc aaatgaggtt atggaaagcc gtgaaaaaga cttatttgaa
     6121 ggtgtgagac attacttaga aactggagaa atttctgaaa aagcatttta tgcaggttca
     6181 catggagatt ggattagtga tttggcagtt tcaattaaaa atgatacacg tagtcgtttt
     6241 ttagttataa ctgaaaatcg tggagctata ccaaacatgc catatgatgc aatggtagaa
     6301 attccagcat atattggtaa agatggtcca gaagttattg ctcgaggtcc aatacctcta
     6361 tttcaacaag gattaatgat gcaacaactt aacagtgaaa aattgatagt agaagcttat
     6421 atagaaaata gttatgaaaa agctttacaa gcattcacat taaataaaac tattccaagt
     6481 atgaatgttg ctaaagaaat tttggatgaa atgatagaag ttaataagga atactggcca
     6541 gaattaagat agtaggataa aggaaagggt ttttatttaa taattagata tgttttcaga
     6601 gtcttttatc aaaatgtatt cacaaacata tggcaatata ttaaaataaa gagtgatatt
     6661 tttagaagaa tggggtgatg caatgaacat tataaataaa attttttggg aagaagatag
     6721 gattggcatg attacaaata atttagaggc agatacacat agccactgta tgttacaact
     6781 gttcttaggg atagaagaca gcattgaaat aacagtcaat gaaaaattag ttaaatgtaa
     6841 ttgtatcata gtagataaaa atatctctca ttcattttca gcaagaaaaa aagtttatta
     6901 ctctgctatt attgaaccta catctatata tgcagaacag cttacctcaa aaatgaatga
     6961 ttttggctac tggatatgtg acaatgatgg attagagaaa ttacgtcaac aaggaacttt
     7021 tttgatagat aactctagta aagaacaata tttaagattt atggaaatgc ttaataacta
     7081 tttgaatata cctataactc tcaaacatta tgatgacaga attactgaat tgctcaattt
     7141 attacgtact tgtaattgtg ataatcatac tatttcaagt tttgcagata aagtgtccct
     7201 atcagcaagt agattatcac atctgtttaa agaacaaata gggatacctt taaaaagcta
     7261 tatcttattt catcaaatgg agtgtgcttt tagagaatta ctttctggga aaaatgttac
     7321 cgaagcttct atgactgctg gattcgatac tccatcacac tttgcaggga cagttaagcg
     7381 aatgatggga atgcctgttt ctttatcttt gaaagatagc gaatttttga aagtatatta
     7441 aatattctcg ttatataatg aagttgtact aagaaagggg ctttgttatg aataaagaat
     7501 atttagagga aacaaaaatg ttaaattata atgaaccaca acttaagctt ttggtatcat
     7561 caaagaattg gttggaatta gatgattttc ataaaataaa atctatttat gagtttgtac
     7621 agaatgatat tttatttgga tataatgctt ctgatatgtt aagcgcaaca caggtattaa
     7681 atgatggtat gggacaatgc aatacaaaag ctactctttt aatggcatta ttacgtgcag
     7741 tgaatatccc ttgtaggctt catgcttttg atgtaacaaa agattttcaa cgaggagcta
     7801 catccaaatt gatttcacta ttagcaccaa aatatattct tcatacttgg gtagaggtat
     7861 tttatcaaga taggtggatt gctcttgagg gtgtcataac tgataaaaaa tatttagaag
     7921 caattcagaa gaaatttttt aatcatggtg gaacatttaa aaagtatgca atagctacaa
     7981 atgatttgaa aaatacatct attgattggg atggtaaaga tacttttatc caaaaagaag
     8041 ctatcgtgta tgattatggt atttttccat caccagacgt atttttttct actcattcac
     8101 aacatatgtc aaaattaaaa aatttcatat atgtacatct tatcagaaaa ataatgacaa
     8161 aaaatgtatg taaggctaga aacaattata ttgataaaaa cgaatgagtg tttaagaggt
     8221 aactatatag gaaatatata gaataattaa aactgtactg gttaaaatga ataaaaagga
     8281 gttatcacag agcaaactta aattgttcat aggataactc cttttctgta ttaacttcta
     8341 ataatttgat gtctttttat aaaacatata gatacaatta aaccagtaaa tgcaagagta
     8401 gctgctattg aaaatgcaac atctattcca tgaatgttag acagcatgtt tgaactcata
     8461 tgagctttct tagtagcact tgtcattata gtaataagta tagcagaacc tatagctcca
     8521 gatatctgac ggagtgtgtt attaattgct gtggcatgag atataagttc tctatctaat
     8581 gttttaattc cccatgtagt taatggcatt aaaaccattg aaataccaat catacgaata
     8641 caatacatca atgacacatg aattaaagat gtatctctgc ctaaaaaagc gaaggataaa
     8701 gttcctaaaa acaaacatcc tgttcctaat attgatagag catgaggtcc atatttatct
     8761 aaatgacgtc ctgcaattgg attgagtaca accatcaata tagcaccagg taacattaat
     8821 gaacctgaag ataatgcaga aaatcctctt acagattgaa cgtacagagg aatcattaat
     8881 gtagcagaca tcattgaggc atatacaatc ataattaata ttgtagaaat aagaaatggt
     8941 ttatttttaa aaaccctcaa ttctaaaaaa ggttctttag attttaattg acgcaatgta
     9001 aataaaatta atgacataat tcctattaat attggtaaat atgtggcaat attagtccac
     9061 ccataattac cttgattagt aaatccaatt aataaaccac caaaacctag agatgatagt
     9121 actattgaag gtatatctaa ttttagtttt tgagtttccc caacatttct taatagaata
     9181 aatgatagta taacatctaa tatagcaata ggggaaagaa acagaaataa actatgccaa
     9241 ccaaatgaat ctactaacca tcctgataaa gttggaccta ttgctggagc aaatcctact
     9301 gtaataccaa ctaaactcat agctgcacca cgtttttcct cagggcataa atagagtata
     9361 ataacttgta aaagtggcat tagagaacca gaacccattg cctgcaacac acgagcaatc
     9421 aacatagttg aaaaattatt agaaaaaagt gcaataatac atcctataga aaagatagac
     9481 attgatgtaa taaataactt tctagtacta aatcgattta ttaaataagc tgttgtgggt
     9541 acaataatac ctgcaataag tagataaatt gttgttaacc actgaccaat atcagcagta
     9601 attttaaaat ctgacattat attaggtaat gctgttgaca atagtgtttg actgagagaa
     9661 cttataaaag caccagtcat tacaattgct attattaaat ttcctttttt attgttactt
     9721 tgggtttcca taatttataa cgtctccttt tttaattgat ttttaatgct gttcaataaa
     9781 agtacttttg gtcttaaagt gtcttctttt tctaaatatg caatcattgc atccataatt
     9841 aaagaatcat attgttgatt ttttccttcg acgtataatt tttttattgc ttctagtaat
     9901 ttaggatttt caaggagaga agtatcagtt ttgattagtt cttctatatc ttgtatcgtt
     9961 ttaaaaaata gcgatttatt atgaagggaa aagtaaactt catcttcttg tttatgtaaa
    10021 aacattgatt tttcaattaa aggatttaga ttatccatat tttctacaac taaatctaga
    10081 atttgtatag taaatttggc aataaagtat acatttattt ctcttttttg tacatgaata
    10141 atgtatataa actgatgaat tagagcatcc aataagaaaa ctaaatcaaa tataattttt
    10201 tctattttat caccatagtt atcaaataag cttagtttgt attcattcaa taagttagct
    10261 cttgattttg tttgcaattt acttgtagcc ttacgttgct cttcagaaaa gttttctctt
    10321 atataggtat ttatggtagt tctagaaaaa atatttttcc atacagcaat tatctttctg
    10381 tttaactttt ctgatgatgg aatgtcatta ttttcattta tggaatgtat aatttctaaa
    10441 aacttttcat cataataatt gattatttcg cagataaaac tttccttagt ttcaaaatgc
    10501 ttgtaaaaag tagctttaga cattttacac tcttttacaa catccgatat tgaagtattt
    10561 tgaaaacctt tttggtcaaa aattttttgt gcaatagtta taatgagcct ttgtttttct
    10621 gtcactttat tatctccttt cacgcaaaaa cgtttagtac tatatagtac ctacaagttt
    10681 tgataaatat attatacatc atttggaaaa aaaatcaata tatagactgt aatatgaaat
    10741 ttttgaaaaa tttgttgtta tttttagtcg tataaaaaag aaacttaaga gtttcttaag
    10801 aattgtttaa agaagttgca ataaattagt ggtatgatta tagtatgaaa gagaatgtct
    10861 tgaaattttt ctgtatcaaa ttccaattat ggaggtgtag tatatgaaaa agaaaatttg
    10921 tgtagcaata atttttgctc tatttatagt ttttgtagaa atgtttattg atagctctag
    10981 cgaaggaagt aaaattatga cagctgaatt aagtaaatac aggaaaggcg taaatgtatt
    11041 atcaataaac aatattatag tagcgaataa aaaatatagt ttgcccaaag attattctcc
    11101 tcaggaaagc agtgaagcga gagatgcttt ttataaaatg aataaagatg ctcaaaagtc
    11161 aggattaaat ttaaaagctt ttagtacata tagaagttat gaatatcaag atagattatt
    11221 taagtcatat gtaaaagaac atggagaaaa agaagcaaat agattttcag ctaaacctgg
    11281 agagagtgag catcaaactg gacttgcttt tgatattgga ggagatgacc agtcttgttg
    11341 ggcaaataaa aagttcaaca atacaaaaga agctaagtgg ttgtatgaaa atgcatataa
    11401 atatggtttt atacttagat atccagaagg taaagagaat ataacaggat atatgtatga
    11461 atcttggcac tatagatatg ttggaactga acatagcaaa aactttgcaa tgaataattt
    11521 gactttagaa gaatatttac atataaatta agagatggtt tgttaaaatt aggttaaaat
    11581 aaaaaataac ctagactatt agctgtgtaa agtgatataa tgtaaataca atttaagata
    11641 actactcgaa ggggagtagc ttacacgaaa gtggtaatta atcgtcaaga cagtagaaat
    11701 actcggttaa ttagcaaggg attccttgat agcaagacct tcaaaataag cattaggtat
    11761 gtttgttttg gggtcttttt ttattcaagg aatttttatt taaaaaaatt taaatttcaa
    11821 gtatacataa gagcccccaa acataaaata aatttattga agaggagggc aacaaatgtc
    11881 ttacaaaagt acttatcaag aagtaattaa aaatttagac tcaagtttat ctggtttgag
    11941 taaagaaaga gcagaagagt tgttagaaaa aaatggagct aatgaattaa aagaagcaga
    12001 taaggttcct acttataagc ttttcctaga aagttttaaa gacccattag taataattct
    12061 attgatagct gctttagtac aaatattttt aggtgaaaca gtggagtcta taataatttt
    12121 tgcagttatt attataaact ctgttctggg tgttgtacaa acaaaaaaag cagaaagttc
    12181 attagaaagt ttgaaaaatt tatctgcacc aaacgcaaag gtaataagag acaataaaaa
    12241 aatgacaatt ccagcaaaaa atctagtagt tggagatatt gtattcttag aagctggcga
    12301 ttacatacca gcagatggaa ggcttataga agcacaatct ctcaaggttg tagaagggat
    12361 gttaactgga gaatcagaac cagttttaaa gcatatagaa aaaataagtg aagatgtaag
    12421 tttaggtgac cagaaaaata tggtatttag tggtgctgta gttgtttatg gtagaggttc
    12481 ttttgtagtg actggaacag gtatgaatac tgagatgggt aaaatagctg ggcttttaga
    12541 aagtgctgaa aataaactta caccattgca acaaaaaata gaggatttta gtaaaaaact
    12601 tggtgtaggt attgtaatac ttgctctttt aatatttata attcaagtag caagaaatta
    12661 ctttatggct ggaaatggag aaatgacaca aattatttta gattcattca tgttctctgt
    12721 agcagtagca gtagcagcta taccagaagc tctatcatca atagtcacta tagtattggc
    12781 tgttggaaca aatagtatgg caaaaaaaca agccataata agaaaattac cagcagtaga
    12841 aactttaggt tcaacaagtg taatatgtac tgataaaaca ggtactctta cacaaaataa
    12901 aatgacagta gttgattatt atatgtatgg aactaataat gatgagttag ataaagaaaa
    12961 agtaaactat tcttatcatg ctaaattatt gactatggtt tctacattgt gtaatgactc
    13021 tcatataact agtgatggta aagaagttgg tgacccaaca gaagtggctt taataaatta
    13081 ttcaagtaaa aatgatttag attatgataa attaagaagt aagtatatca gaataaatga
    13141 aataccattt gactcagaca gaaagcttat gtctactgta aatagtatat atggagatta
    13201 tataatgttt acaaaaggtg ctccagatat tgtgtttagt agatgtaaat atgctttgaa
    13261 aaatggttca aaagtagata taacagatga gataatagaa gaatataaac ataaaaatga
    13321 agagttctca aatagagcac ttagagtatt agcttttgca ataaaagatg tcccagaaga
    13381 agattttata ccttcaattg atgacgaaca tgacatgaca ttggttggaa taatggcaat
    13441 gatagaccca ccaagagaag aagtaatgga tgcagtaaaa gaggcaaaga gtgctggtat
    13501 aaaaactgtt atgataactg gtgaccataa aacaacagca gcagcaattg caaaagagat
    13561 tggtataatg gaagaaggag acttggctct tactggaaaa gagcttgatg ctttgagtga
    13621 agaacaacta tatgaaaaac tagaaaatat aagtgtatat gccagagtct cgccggaaaa
    13681 taaaattcgt atagtaaaag catggcaaca taagaataat ataacagcaa tgactggtga
    13741 tggtgttaat gatgcacctg cattaaaaca agcagatata ggaatcggta tgggaagtgg
    13801 tactgatgta gcaaaagatg cttctgctat ggtattggtt gatgataact ttgcaagtat
    13861 agtaaatgct gttgaagttg gtagaacagt atacagtaat ataataaaat caattactta
    13921 tctatttgca ggaaacttag gagctatagt atccatagta tttgcagtgt ttgcaggttg
    13981 gtcaaatcca tttacagctt tacaactttt gtttataaac ttagtaaatg actctttacc
    14041 agcaattgca ttagggtttg aaaagtcaga aaaaaatata atgaataaac ctcctagaca
    14101 cccggatgaa agtatactag caggaggaac attgaaggca gttgctataa gaggttgtat
    14161 aataggtgta gttacaataa ttgcacaata tataggatta caagtatctc cagaacttgg
    14221 agtagcaatg gcattttcaa cattgatatt agctagaata atgcaaacat tacctgcacg
    14281 ttctaataat caaacagtaa taaaattagg ttttttcagc aataaatacg ttataggagc
    14341 agttttagtc tgtcttgggc tttactcttt aacactaata ccagtgctaa gaggaatatt
    14401 ctctatacca acaacgtttg gattgtacca gttggggatt tgcctagggt tagcattaat
    14461 ttcatcaata ttaatggaac ttaacaaatt aataaataga agtgaagtaa aataatcaaa
    14521 aaattaaagg gcatattgat tttaacaata tgccttttat aaatagtcta atttattcta
    14581 tattatcgat tatttggaat gcttttataa cttcaggtaa ttctttttct tcaggcattc
    14641 taaatcttat aaaattttta tcaagtccag taaaatcact tccagatata actcttattt
    14701 ttaatttagc aaactcttgt tgtaaatcta tgtccttgtt tttatgttca actgtcataa
    14761 ttgatacagt atcactagtt tcagctatat taaggttttt ccatggaatt aataattgat
    14821 tttttatatc ttttgttttt tcttttaatt cttctataaa ttgaacatca tctaataaat
    14881 ttgctgcaat actacgagat atttcagaca cttcgtaagg agtagaaatc tttttaatag
    14941 ggctaacaag ttgttctgga agaactgcat atcctgctct tagtcctgct aaaccaaatc
    15001 cttttgaaaa tgtttttaaa gcaattacat tatcataatt atttagaagc ttgacagctg
    15061 aattttcttt tggcatgtat tcaccataag cttcatctac cataactgct atatcatatt
    15121 tagctgcttc ccttactata ttttcaattg atgataaagg tataatttgt cctgtaggat
    15181 tgtttggatt gtctatgtaa attactttat attctggatt tattttttca ataaattctt
    15241 tttcattaaa cttgaaatta tcctctttct ttaaaagaac ataatcataa gttgcaccat
    15301 gcatttttat atctgtctca tattctgaga actgaggtga atatcctaaa actttatctc
    15361 ccttttcaat aaatagtcta tttaaaaggt aaataacatg tatagaacca tctgccaaac
    15421 aaattctgtc agtatctaat gctataaaat ctttccaata atcgactata ttatctttta
    15481 aagcaattga atgaggatat cctcttatca tatcaaattt taaagcatta aaagcttcta
    15541 cagctttgtt tgagtaagac acggtattta taccttcacc acagtctatc tcgaatacat
    15601 cttctgaatg tccagattct attgcataac tttttttcaa ctgcatttca atagctttgt
    15661 taatttttag tttcatactt tatcactcct tatttagtca ataataaatg ttataaaagt
    15721 tgctagattt aaaatattat gaaaatatat aatagtaaaa atgaatataa atattataat
    15781 agctcacttt atttaatatg attatatatc atatattgta aaatttctag cattataaga
    15841 ctataaattg aaaatattca gaaaaatact acaagaaacg atttattttg ctcttaaaat
    15901 aagttaaatg aatgttatta attttgtaaa ttgaaatagt ttaagtataa agttatgata
    15961 taatctaata agggtaattc tttagattat attaaggaaa tataggtata caataaaaag
    16021 ttatggagtt ggattatggg atataaatta attgtaacag atatggatgg aactttatta
    16081 ggcaataatc ataaagttac agatgaaaat aaaactgcac tacaaaaagt tataaaaagc
    16141 ggtattaatg taactttagc aacaggaaga gccttcgatt cagctaaatg caatgtagat
    16201 tttcttaaag aggacatgcc aataatagct tgtaatggtt cattaataag agagcaaaat
    16261 ggaaatataa tatattcaaa taagattgat acaagaacat gtttaaacat tttagatgta
    16321 ttagataaat acgatattta ctaccaatgt aatagtatag attctatgct ttcgaaaaaa
    16381 atagaaggac gtgaagatag attaagtgta tttttaggct ctgaaacaga agtaattgta
    16441 aaagatgatt tgagagaaga aatctttaaa aaagatatac taaaatttgt aatcatagaa
    16501 gaaaaaaatc catctatttt ggatgaaata agaaaagaat tgagaaaagt tcaaggaata
    16561 aagataacct cttcatggcc aaacaatatt gaagtcatga atgaaggtgt tgataaaggt
    16621 aatgcagtta aaattttagc tgaaaaaatg aatatagaca gagaagatat aattgcattt
    16681 ggagacaatt acaatgacat agaaatgata aagtttgctg gtcttggggt agctatggga
    16741 aatgcagaag aattgattaa acaagaagct gattatgtta cagatacaaa tcaagacagt
    16801 ggagttgcaa aggcaattta taaattccta gagttaaaag aatcacttag ctcttaaaac
    16861 agtatataat aggaaaaaat aggttgacta ttttgtagat agtaattata atacaaatag
    16921 ataaaatata aaaatttaga ttcagtgaaa aagagagtag atatagagaa aatctcagcg
    16981 aattggggga tggtgtgagc ctaatgataa cactttatat tgaaaagaac tttggagaat
    17041 actacttgaa attcagtatg gtagtaggta atctcgcctt aagagaatag agtgggtaaa
    17101 cattgtttac caactagagt ggtaacacgg aatatttcgt ctcttggtct ttttgaccaa
    17161 gagatttttt agtttcattg agttacaaga cttataatta aaatacaaaa aattaaaata
    17221 tgaggtgaaa gttatgaaat tatggggtgg acgctttaga aaagcggaaa atcaacttat
    17281 ggaagaattt aataaatctt ttggctatga ttgtgtactg tacaaaaaag atatcgaagg
    17341 aagtgtagca catgttcata tgcaagtaaa atgtggactt ttaacagaag aagaaggaaa
    17401 gtctataaca gaaggtttaa aagggatact tgaagatgta gaaaatggaa agttagtatt
    17461 ggatggagag tatgaagata tacatagttt tactgagata aatttaattc aaagaattgg
    17521 agatgttggc aagaagcttc atacagcacg tagtagaaat gaccaagttg cagttgatat
    17581 gagattgtat gctaaagaaa aagcaaatga tttggtggat ttaatatctg aatttaaagc
    17641 tactataaaa gatgttgctg acaaaaaccc agtgatgatg ccaggctata ctcatcttca
    17701 aagggcacaa gtagtgacat ttaagcatca cttaatggca tattatagta tgtttgatag
    17761 agatgagaaa agaataaaaa atgctataga aatattagat gaatcacctt taggctgtgg
    17821 agctttagca ggaactacgc atgatataga tagaagtata acttgtgaac aattaggatt
    17881 taaaaaagta gtagataatt ttatggatgg agtaagtgat agagattatt tactagagct
    17941 aatgagtgat ttttcaatta taatgatgca cttaagtaga ttaagtgaag agttgatact
    18001 ttggagttct caagaatttg gatttgttga gatagatgat ttatatacaa ctggaagtag
    18061 tataatgcct cagaagaaaa atccagatgg tgcagagctt ataagaggta agactggtag
    18121 agtatatgga aacttatttg gtctttttac agtaatgaaa ggaataccat tagcatataa
    18181 taaagacatg caagaagata aagaaggttt tttcgatagt gtacatactc ttgaaatgtg
    18241 tatacaaata atggatagaa tgatagccac actaaaggta aatgaggata aaatgaaaca
    18301 agcagtaaaa aatggttttt taaatgcaac agaagttgcg gattatttgg ttaaaaataa
    18361 tgttgctttt agagatgcac atggtatagt aggaagtatc gttatatatt gtgaagataa
    18421 taaaaaagca atagaagatt taacattaga agaattacaa aagttttcag atgcttttaa
    18481 ggaagatatt tatgatttta tcgactatga gagtatatta aataagggaa taaaaaagaa
    18541 tttaaaataa tgtatttatt tttaaaagct gtaaatttga atgttatcat ttaaatttac
    18601 agctttttat tgtgtaagat tttattatat aaaagtaaga aacttaaaag gagtagtatt
    18661 taaaataaat acaaataaaa aattattatt aaaatgataa ttaatatcaa taagtattga
    18721 caaaattatt tctaaatact attatatata atataaataa taatgaaaat caatatcaaa
    18781 taaatgggag gaaatctaat gaacttatta gtgattggtg gtcatgaaag aatggaaaaa
    18841 gattatatgg ttatggccaa gaaaaaaggt tataagacaa aggtatatac taccatgtca
    18901 tctaaattaa ataattctat aggaaaacca gatgcagttg tcatacttac atcaactgtt
    18961 tcacataaga tgtctagaac ggttgagtct caagctaaaa aacaagatat attaatcgta
    19021 aggcataaaa atagtagtaa ggtagcattt aatgaatgtc tagatatggt tgatgaatgt
    19081 ctgggaaatt gttcaaaatg taagcttaaa gacaaacaaa aacaaaaaaa tatataggta
    19141 taaaaaatac ataaacataa aattaaaagt actgagataa tagttataat tattatctca
    19201 gtacttttag atttataagg ataagtatag tataaatttt ttattataat ttccaactac
    19261 ttgttacttt ttcatatttt ttttacttct agcttttttt ctttttttct tctttatatt
    19321 tgaagctatt ataaataaaa ctaaaagcac tagaatacct gcaataatct taacaaaagt
    19381 cattaaaaca ttattttcag tcaaaaatgg aagactgcta tcaagattat tttttgcaat
    19441 taagtctata ctgttttcta gtttaccatt attatataca tttaaagtac caaccttagc
    19501 tccttttttt ataggcaaat caattttatt taactctgtt ttggtatcat aattgccttg
    19561 agattcatct ttttctaaga atatcctata actatttttt ggttcataaa tcaattcatg
    19621 ttgttttgta aacaatactt ttttagtatc tgtgtatttt tctttatcaa caatagtagc
    19681 acacttaaag ttatcaaatc cataatcaat cagaatacgt gagtctaaat ataaatcagc
    19741 attagttgaa ttaaatacag caacgataac tcttctgcca tcttttacag cactcgataa
    19801 aagacattta ccagcagcat cagtatatcc agtttttata ccatcaacta tatcatattt
    19861 tatatttata ggttgacctt tgtatgtaat cttatcatgt gatgtcaaaa acttattagt
    19921 gtttacaaag tatctctcat aaggatatgc ttttgtagct tcaaatttaa gtgacttagt
    19981 ttttacaatt tgtctaaaag tatcattatt catagcttct ctagctatga gagccatatc
    20041 atgagctgtt gtgtagtgat ttgggtctgg taatccatga ggattattaa aatgagtatt
    20101 agtagctcca attttttttg cctcagagtt catgaggtca ataaattttt gtacattgcc
    20161 accaccaaca tatctagcta atacaaaagc agcatcatta gcagaatgaa ccaaaagagc
    20221 atctagtaac tgctttacag taaaagattc cccatttttt agatacatac tagagccatc
    20281 tatttgaggt aaatctttaa tttctattac ttgatttaaa tctttaacct cctttagaac
    20341 taaacaagct gtccatactt tagttgttga agctggataa agcttagaat ttccatcctt
    20401 attatatagt attttgccag actcatagtc cattaaaact gcatgtttag caactatagc
    20461 tggttcatta tctgcaaaag agatattaga gcttcctaaa aatgaagtaa atatcaataa
    20521 acagattaat aaaagtgata aatttctttt cattgtgagc ctccaaattt taaattcatc
    20581 ttttagtata gcatatattt gtcaataatt aaaagaaagg agtgattaat atatgaaaaa
    20641 aaaggtaaaa tttatttcgt tttttgtttt atttatttta ataatatcat caataatcgt
    20701 ttataaatcg cttattttca aagatgatat ttatgttgta agtaaaaaaa atttacctaa
    20761 tgaaaaaaca catgttgtag agaatgaaat taaaggtgaa gataataatc acagtaaagt
    20821 aactaaagac atagataaaa attttcaaaa taataaaata actatataca taagtggagc
    20881 agtaaataga cctggaattg taactataga gtcagacaag agactttatg atgctgtaga
    20941 attactagga ggaacgacaa aagaagcaga tttaaatgga gtcaatctat cagtaagatt
    21001 agaagatgaa caacattaca taatacctaa aataggtgaa gcaacatctg ttacaagtaa
    21061 tgatgattcc aataaactaa atcagaagaa tgagtcaaga ggtgagccta aaaataaatc
    21121 caataaaaat atatctaacg aatcaaaagt aaatataaat gtagcgacga tagaagagtt
    21181 ggattctcta cctggggttg gagaagctac agccaataaa attttacaac acagagaaga
    21241 aaatggacag tttagttcaa ttgaggaaat aaaaaatgta aatggtattg gagataaaaa
    21301 gtatgaaaat ataaaagatt taatttgtgt agactaaaat ataatacaaa acttacatat
    21361 agatatgtta ttataaatat gtatgtatat gtgtgtacat gtggaaattt agtaaaggag
    21421 gcacttttat gaaaaaagaa caaaaaaaac ttacagagat gacaacagca ggtggttgag
    21481 ccgctaaaat agggccagag gttctggcat cagttttatc tcaattgcct aaaaatgata
    21541 atatagaaaa tttattagta ggactagata cagcagatga tgctgcagtt tataaattaa
    21601 atgatgatat ggcacttatc caaactttag acttctttac accaatggta gatgacccat
    21661 acgtatttgg tcaaattgct gcatcaaatt cactatcaga tgtatatgct atgggaggca
    21721 aacctttggt tgctatgaat attgtttgtt ttccatcatg ccatgatatg gatgtacttg
    21781 cagaaatatt aaaaggtggg tttgataaag tgaaagaaag tggagcactt ttggttggag
    21841 ggcatactgt agatgacaaa gagcctaaat atggtctttc agtttctggg attgtaagtc
    21901 caaataaagt tttatcaaat gctactgcaa aacctggtga taagttaatt ataacaaaac
    21961 caattggagt gggtgtatta aatacagcca tgaaagaagg tatggtagaa caacatatag
    22021 cagataaagt aatagaaata atgatacatt taaataaata tgcagcaatg agttttgaga
    22081 aatttgatgt aaattcggtt acagatataa ctggttttgg tctacttggt catactttag
    22141 aaatggccaa agcttctgaa gtaagtattg aaattgaatc aaaacatgta ccaattattg
    22201 aaggtgcaat agaaatggca caaatgggaa ttatacctgc tggtatgtat aaaaatatgc
    22261 attatgtatc taaagatgta gaagttgtag gaaatataga agttgcagtt caagatattt
    22321 tatacgaccc acaaacatca ggagggttgt taatttcagt aaaagaagaa cttgcagagg
    22381 agcttgtaaa agatatgaaa ttaaatggag caatagaagc taaaattata ggttctgtag
    22441 ttccaaaagg ggaaaaatat attacagttt tataaaatat tatagagaaa gagtgattgt
    22501 tttgagcaaa agagaattgt ttgcaatgtt gccttcagtt gatgaagtat taagtgataa
    22561 tagaattgta gaaataataa atgaatatcc tagaagttta gttttagaat ctgtgagaga
    22621 agtaatagat ttaaaaagac aatttatatt aagattaaaa gaagatgcat caaactctgt
    22681 aacaatagag tttggtgaaa taatagaatc tgcaatagag agagttaagt taaattactc
    22741 attatcttta aaaaaagtta taaatgcaac tggtacagtt attcatacaa atttaggaag
    22801 gtctttactt agtgaagata taaaagatga attatggtgt gctgcttcaa ggtattcaaa
    22861 cttagaatac gatttggata atggagaaag aggttcaaga tactctcatt taactagtac
    22921 aataaaaaga cttactggag cagaagatgt actagttgta aataataatg cagcggcagt
    22981 acttcttgta cttagcacaa tggctaaagg aggagaagca attgtatctc gtggagaatt
    23041 agttgaagta ggtggttcat ttaggattcc aagtataatg gctcttagtg gtgcagaatt
    23101 agttgaagtt ggttcaacta ataaaactca tttgaaagat tacaaggagg ccattacaga
    23161 agatacaaat gtattgatga aggttcatac aagtaattat agaataatgg gattcacaga
    23221 gagtgtttct atagaagaat tggttaattt aggtaaaaaa tataaactac ctgtaataga
    23281 agacttagga agtggagttt ttatagattt atcaaaatat ggtttatctt atgaaccaac
    23341 tgtattggat tctattcgac aaggtgcaga tgtagtaact tttagtggag ataagatgct
    23401 tggaggtccg caagcaggta ttatagttgg gaaaaaagag tatatagaaa aaatgaagaa
    23461 aaatcaactt actagagcac ttagagttga taagcttaca atatgtgctc ttgaagcaac
    23521 attgagaatg tatttagatg agacaaaagc tatagaaaat atacctactt taaaaatgtt
    23581 gacatataaa attgaagaat tagaagtaaa agcaaataaa ctatttgaaa aaataactgc
    23641 tttaaactta aatgcaaaca taaatataga agatggattt tcacaggtgg gaggagggtc
    23701 tatgcctctt gaaactataa gtacaaaggt aatatcaata actcctgaac atatgaatgt
    23761 ttcatcttta gaaaaaaaac taagacttag tgaagcacat ataatagcca gagtgtatga
    23821 taataaatat gtactagatg taagaactat atttgatgat gaatttgatg taatagttga
    23881 ggaattgaga aaagcattta attaaataaa tttgagttaa taaatattaa atttagaagt
    23941 ataagtatga agttttgatt aaaaaatagc tacacacact tgtgacttta gtcatagcag
    24001 gtttagaggg ggcaaaaaat gaaaaatgta gtaattggaa cagcaggtca tattgaccac
    24061 ggaaaaacta ctcttataaa agcattgact ggaagagaga ctgatacgtt agatgaagaa
    24121 aaaaagagag gtatttcaat aaacttagga tttacttatt ttgatttacc aaacaataga
    24181 agagctggaa ttgttgatgt cccaggacac gaaaaattta taaaaaacat gttagctgga
    24241 gcatcaggaa ttgatatagt tttacttgtt gtagcagcag atgaaggggt tatgccacag
    24301 acagttgagc atttagatat tctaagcttt ttaaatatca aaaatggaat agtggttcta
    24361 actaagtctg atactgtaga tgatgaattt agagagctag taaaagaaga tatttgtgaa
    24421 aaaataaaag gaacttttct tgaagatgct gaaataattg aagttgattc tatatctaaa
    24481 agaggtatag acactcttat tgaaaaaata gataaaatga gtgatgatat agaagataaa
    24541 aatgaaaact ctccagcaag acttaatata gacagagtat tttcaataaa agggtttggt
    24601 actgtaataa caggcacttt aatagaagga aagataagta tagaagatga tttggttata
    24661 tatccacagg tattaaaaac aaaaataaga agtatacaag ttcatggaga aaataaagat
    24721 acagcttatg caggtcaaag aacagcaatc aatatatcaa atgtaaaagt ggatgatata
    24781 aaaagaggag atgttttagc tgcaccaaac tcattggagg aatcaatgat gttagatgta
    24841 aaattatcat tggtaaatca tttagataaa ggattaaagc actgggatag attaagattg
    24901 tatcatggaa ctagagagat actttgtaga gcagttccac tagataaaga attaattgaa
    24961 agtggagaaa gtggctatgt tcagttgaga cttgaagaaa gcatagtatc taagaaaggt
    25021 gatacgtttg tagtgagacg ttattctcca atggaaacta taggtggagg tgtaataatt
    25081 gacccatctc ctaaaaaaca taagaaattt gatgaaaaag ttatagaagc attaaaaata
    25141 aaagaaaaag gcgaactaaa agatataata gaagagtact taaaaaggaa tttaaaaaat
    25201 tatccaaata taaaagaaat aatgagctat agtggtgctc atgaagaaga tgtaaaaaga
    25261 gcgcttgaaa cacttatatc agaagataaa gtatttatta taggaaatat gtatatgcat
    25321 ataaatcaat acaataaatt aaaagaaaat acaataaagc ttttaagtga gtaccataag
    25381 aagtataggc ttagaaaagg gatactaaaa gaagaagtaa ggtctaagat agagagtaat
    25441 tttaagacta gggagatgga tattttatta gaaaaattgt ctacagaaaa tgctataaaa
    25501 atagaaaata atatagtatc tctattagat tttgaggtta ttttaaatga taagcaaaaa
    25561 gaaatagcaa aaaaaataga aaaaagatta aaatcatgtg gagtttcttc tatactgaca
    25621 atagatgaag tttctgaagg aaatcataac tatgctgaag tattggagtc aatgataggt
    25681 aataaagtag agaaattaga tgatttgtat ataatggaca aagatattta tgaaaatgca
    25741 aagaacattt tgataaatta tataaaagaa aataaagaaa taacattagg agaatacaga
    25801 gatttgattg attctagcag aaagaattgc atgattatat tggaaaactt cgatagaaac
    25861 aaaataacta aaagagtaga aaacaaaaga attttgtttt agcagaaaat gtctgttttt
    25921 gctttaaggt ataaagaagg aattataatg tcgagtatcg tataattaat tagtataaaa
    25981 tattaattaa gaggaagtgt aaagtattga agaatataat acctgatgta aaaccagata
    26041 taaaagaaag agaagacatg agccttttat ttataataat gggctcagta cttttattat
    26101 ttattataaa agatatagag aaatatgaaa ctttattaca tgccatatat acgatatttt
    26161 tagtagctac aggagtaatt atatttaata ctacaaaatt taaagtagat tcattttcta
    26221 catttttagg attaattttt atagctactg gagtattgga gtgtatatat ttatttaatt
    26281 ccttggggat taaaactaag tctataatgg agattaacat cacaatatca gcaataacag
    26341 atttatttcc tatactgggt gtatacttaa gttttaaatt tgttaaggat aacaaacaaa
    26401 tttattcaag tgtaatttta tttatagcaa ctgctacatt aacaataaca tctttattca
    26461 taatatcagg aatttgtgac tatctgggaa atggtaaatc acaatatgct ttaggagtta
    26521 taatatctat attcataata attatttcta tcattagtgg aattgaatta aattcaagtt
    26581 caaataaagg taaatgggaa tatgatgaaa aaaagatttt taacagaata atagtaataa
    26641 taatcttatc aagagttcca aatctattgc atatagttat tgataataga aatgtagaga
    26701 ggatattatc acaaataata ataaatgtag ccttatatta tttatataat tacatagtat
    26761 ctaagaatat taagaaaaca gcactggaat tgcatgatac gaatgaagaa ttaacaaaaa
    26821 aaacggaaag tttaaaagaa aagaataaaa aattaatcta tgagacagaa aagatagagg
    26881 aacttaaggg aatacttatg aaaagagaat ctaggctaca atctacatta gatgtagcag
    26941 taaatagcat agtagtattt agcaaagatg gagaaattac atatgcaaat aagagcttta
    27001 gaaatacttt tggagactat aaagaacaag ataaattgac tgataaaata aaaaacttca
    27061 atgatttgat agaaagcatt cattatgtgt ttgttcatga gaagaatgca gagaaactta
    27121 ttagtacaag cgataataaa gtttatcaag ctatatttgc acctttaatt attgcttcac
    27181 aaacagaagg agtattgtgt gtactagaag ataagactaa gaaaaaggag tacgaacgca
    27241 aacttattga agcaaataag agaagtaaag attttttaga aagtattgga gatggcatag
    27301 tagtgcttga aggtaataac aaaatatatg ttaataatgc atgtagggaa atttttaaaa
    27361 ataatttaga gagtattgat ttttcattag tatgtaaaag tgaagaaagt atagaaaaaa
    27421 gatatgtaat agatggtgaa gtaaagtatg tagagatgag tttttctcag tatactaatg
    27481 ctggtactaa taaaactatt attgtaatac gtgatacaac ctctcgaaaa atatctcaaa
    27541 taaaacttga ggaaagtcaa tcatcatatt caagatttat agatatatta cctgatggca
    27601 tatgcttatt aaaagaagat ttaagtataa actatgcaaa taagtcgttt ttagatatgc
    27661 tagcttttac aaacatagat gatataaaag attctaatat taaactatta atgaatgcga
    27721 atactgaaga aaaaatgaaa tttacggata atatggctaa agttttaaat gaaaatagaa
    27781 gtatactttt actggaacat gaattgataa atagttatga tgatatagta gaagtagaat
    27841 taagtgcatt accttttgct atatacaata ctagatacat aatgcttatt ataaaagatt
    27901 tagtacataa gaaatattcc gagcaagcag aaaaagaact gcttgaaaga tttaaaacag
    27961 ataaaatcaa aacggagttt tttgcaaata tgtctcatga actaaaaact ccactaaatg
    28021 ttatatctag ttcaaatcaa ctggtagatt ctttctatag aaatgaaaag ataaaagatt
    28081 acaataacaa tattaaatct catgtggatt tagttagaca aagttcttat aggcttcaaa
    28141 gattaataaa taacattata gatttaacaa agatggagtc tgggttctat acactaaaac
    28201 ttgcgaaata caatattgta tctgtaatag aagacttatt tatgaatata gaagagtatg
    28261 cacttagaaa agacataaaa attctttttg atacagacct tgaagaaatt aatgtttata
    28321 tagacaaggt tgaaattgag agaattatgt tgaatctatt gtcaaactgt attaagttta
    28381 ctgataatgg aggctggata tatgtaagta ttcattataa aatagataaa gtaatcataa
    28441 gtgtaaaaga cactggagtt ggaataccac aagataaatt agagttgatt tttgaagaat
    28501 tttcacaagt tgataagact ttatccagaa atactgaagg aagtggaata gggcttgcta
    28561 tagttaagaa tctagtaagt cttcatggtg gagatataga agtagtaagt gaagtgaata
    28621 aaggcacaga gttcttaatt tcacttccaa tgaagggatt tagtagtgaa cattacacag
    28681 aagataagag aatatataat attcaagaaa aaataaaaat agaattttct gatatatatt
    28741 attaaaaatg attatgagca aaaataaaat aaaaatacta tttgtttaaa tatttaagtt
    28801 gtaagaaata ggctatcata aggtaaatta ttatgatagc ctatttttag ctaggtttta
    28861 tatctatatg tgagaacata taatctctaa attaccttca aaactaaaat ctccaaagtc
    28921 gtttggcagt ataaaatgca tacccttttt taaattatat tctacagaat tgtgtataag
    28981 ttttccattt ccatccaaca cactgtataa agaaaaaggt gtattgtgtg taaaattaca
    29041 ttttccaaaa acatctaatt tataaacact aaaaaattcg tttgacacaa atgttgtgca
    29101 tttaaagtct gatgttgata cgattttata gtcagtatcg aaatttatat gaggaacatt
    29161 cgtaacatct attgattttt gaacatgaag ttcacgctta ttacctgaat tgtcagttct
    29221 atcataatca tagactctat aagtagtatc tgagttttgt tgagtttcta aaatcaatgt
    29281 tcctttgcat atagcatgta tagtgccact tggaacatag aaaaagtctc ccttttttat
    29341 attaaccttt ctaagtagtt tgtcccattc attattattt accatattta caaattcttt
    29401 atgagatttt gcattatgac ctattataat ttctgcatca tcagaacaat ctattacata
    29461 ccaacactct gtcttaccta actctccatt ttcatttttt ttagcatatt catcatttgg
    29521 atgaacttga actgataaat tatcatttgc atccaatatt tttgtaagta aaggaaactt
    29581 atcaccaggt gtgtttccaa ataattctct atttttattc cacaattcag atagtttttt
    29641 acctttatat tttccatttt ctattaagca atctccattt ttatgagagc ttatagccca
    29701 acattcacca gttgttggcg aatctatttc ataattaaat ttatctttaa gtgcagtacc
    29761 accccatatt ctatccataa aaataggttt taaaaataat ggttccataa tcctgttcct
    29821 cctatgatat taaatttatc ctacaaataa ttatatataa atagattata aaatcaatag
    29881 taaaaaagtt ttgtcccttg aaatgacata aagtataagg agttattaaa taagataaac
    29941 tatgatatat aatataaagt tctaaattgg agtgataatt atggagttgt ttaaggcaga
    30001 aagcaaaaaa tataaaaaag aatttttaaa attattggag caatgggtta gtataccttc
    30061 attttatgat agaaaaactg tatctaaaga catgcctttt ggaaaaggag tatatgatgc
    30121 attaaactgg tttgaaaatc taggtagaga aaacaatttc aaagtaaaaa atatagataa
    30181 tcatgctgtt caaattgaat atggaaatgg taaagagtat gtagatatat ttggacattg
    30241 tgatgttgta aatccaggtg aaggatggga tagtgaacca tttaaactaa atattatagg
    30301 tgataaatta gttgctagag gtgtatctga taataaaggg cctatgatag tgaatttttt
    30361 agcactaaaa atgataaagg atttagatat taatttaaaa agaaaggtta gattaatagc
    30421 tgggggaaat gaagagagtg gatttaaatg tataaagcat tattatagca aagagccata
    30481 tggtgtatgt ggatttactc cagatgctaa atttcctgta ttaaatggag aaaaaggtgg
    30541 agcaataata aagttaattt caaatataga cgataaatct ttatatataa gtggtggaat
    30601 agagtttaat acaataccag ataaagttta tataaagaat gtagaaaaat tgggtaaaga
    30661 taatatatgt tttgatatta acaatatatc gataaattat gataatggca attatattgt
    30721 gtatggaaaa ggtggtcatt catctaaacc cgaaaaatca ataaatccta tattagctac
    30781 aattaaactt ttgtcagaaa atattgatga aaaatggaca aaagatttat ataagttgat
    30841 aaatcaagat aatataaatg gaaacttatt tggtttaaat attgagggga aatgtggaat
    30901 tttatcaatg gtgccaacaa taataaatat agtagatgga aaattagaag tagttttaag
    30961 cgttagatat ccagaaatat taaccattga agatataata aaaaaattta atttgtatat
    31021 ggagcaaaat aatattaata aatttgagct tattggggaa aatttaaagc aagcaaatta
    31081 tatagataga aattcaaaac ttgtaagaag tttgcatgat atatatataa agtattctgg
    31141 agatttaaaa aatgatgtta gagtaactag tgcaggaagt tatgcttctg aaatgaataa
    31201 ttcagtaata tttggttgtg aatttcctga tggtagcttt ggcaatgtcc atagtgcgaa
    31261 tgaatttgct agcttagata gatttataac tgcaataggt atatatgcag aggcaataat
    31321 tacattatgt aataaaatat aaatacaaaa tgtcccttat atagacatat tatatataca
    31381 attaaaattt tacttgatat ataattgaaa tcaaagagaa ggtgatacaa tgataactaa
    31441 gaaacatgca ataatagtaa agagtttaag caataaagat ggatatatga catcaaacga
    31501 attagctatc aaattggatg tgtctactaa aactattaaa agatatatag cagatttaaa
    31561 tagtgtttta agcaaatatg atttggtaat agcttcttct agaggtatag gatacaagtt
    31621 aagcggttct aaaaacaata tagccagagc tgttaaagaa gcaaataaat atattgatgg
    31681 ttttttagat gactctgaag agtcaagaat gtcaaatata atatgtatgc ttattaatag
    31741 aaattatatg agtatagaag caatggcaga agaattaaat ctaagtatag ccgctataaa
    31801 taaattgtct agtaagctta aaagaaaact tgaaaagtat gacttagtta taaaatctaa
    31861 accttactat ggaagctata tacatggtga agaaattaat ataaggcaat taattactga
    31921 ctatgcaata aaattagatg aaaaaagtaa agtgaaggtg tttttagatg acattagtga
    31981 aaacgatata cactgtatag aaagtatttt agaaaagcac ttaagagaaa aagatacaat
    32041 aatttcagat aaagatttta atcttttgct ttcaaaaata atcgtttctg tatttaggag
    32101 taagagaggt catagtaaca atataaactt aatggataca agttatagat ttcataacta
    32161 ctactttata gaaaatctaa tgaaagaaat atctgataaa attggattta aacttattga
    32221 agatgaagta atttatattt caaattattc tggtgtaata gcctataaag gtacacaagg
    32281 aagaaaaaac acaagtgaaa ttgaggaacg aataagtagt gttataagta gtgcacttca
    32341 agatatattc cttatttcag gtagtgatta tacaaaagat gatgagttta tggttgcaat
    32401 ctctgaccat ataaagagat ttttaaatag agctagagct aatgtaaaat caaataatcc
    32461 tttacttcat caaataaaag agaagtttcc tatagctttt aatttagccg tgtttttatc
    32521 aaataaacta gaaactgagt ttaatttaaa acttgatgaa gatgagcttg gatatatagc
    32581 tatccacttt gctgcttcaa atgaaagaat gaaaaaaaat actagtaaga aaatttgtat
    32641 agtttgtcat tatgggatag gaacaggaca acttatttct gaaaagctaa aacagaatat
    32701 aagtgattta agtgttgtag gtgtttatcc agttagatat ctcgatatgg ctataagtca
    32761 agatgttgat ttgatagtat ctaccgttga attaaaagga tatgaaaaac ctgtcttata
    32821 tatagaaaat atatttgatg atagcttaat tgaaaacgta aacaaagcct tttatgaaaa
    32881 ggaagaaaga agaaagataa taagcaacat gtttgatgaa aaagcttttt tcagtataaa
    32941 ggctaatact aaagaagagg tcataatttc actttcaaat aaactaaaag aaagagattt
    33001 tatagaggaa agttctataa aaagtataat agatagagaa aatatttcat caactgaaat
    33061 aggaaatcta gtagcaatac cccacacaat agtgaaggga gataaaaaat ctataatagg
    33121 agttggaata cttgaaaatc caataatttg ggataagcaa gaggttcagc tagtatttat
    33181 ggtatttttt aatactaaag aaaaacataa tttctcgata tttaaatacc tttataattt
    33241 tataaaagat gaaggcggag taagaggaat tattaaaata tgtgacttta ataaattaat
    33301 ggcactgata ggtaattaag gaggaaatta ataatgggaa caaacatatt taacaaagaa
    33361 tatgtatttt taaatgttga tgctaagtca aaggttgaag ttttaaaatt tatatcaaaa
    33421 aaagctaaag acttaaactt ggcagaagat gagagcttag tatatgaagg tttaatggct
    33481 agagaagaac aatttactac aaatttagga gaatctatag caatacctca cactaaaaat
    33541 gatgcaattg aaaatccagc agtagtagta ttgaagttta atgaagatgt tgtttggaat
    33601 gaaggtgagg ataaggttaa attagcaata agcttactta tgccaggaaa aagtaaagag
    33661 aatattcatc ttaaattatt atcatctctt tcaagaaaat taataaataa agaatttaaa
    33721 gattctcttt taaaaagtga taatgttgaa gaaatatcta acttaataaa tgaagcttta
    33781 ggtttataat taaacactaa ggttataaaa taaaaaaata attaactaaa aataggagga
    33841 atttgaaatg aataaaaaat tagtagcatt atgtgcttgt ccaatgggac tagctcacac
    33901 tttcatggca gcagaagcaa tagagcaagc agcaaaggca ttaggttatg aagcaaaagt
    33961 tgaaactcaa ggtgctgatg gtgtacaaaa tgagttaaca agagatgata tattaggagc
    34021 aactatgata atacatgcag tagcaataac accagaaggt atggaaagat ttgatggttg
    34081 tgaagtttat gaagttgaat tacaagaagc tataaaaaat gcagaaggtg ttataaaaga
    34141 aatagaagaa gatttaggaa tataataaat ttattgtgga ggaaatagaa tggcaattaa
    34201 gaaaaaagtt ataaacagtt caggtgcttc tacaacagga ggaaataatg taggaaaatc
    34261 tactccatct agtccaactc aatcaaaggg aaatggaaaa tggaaagaaa taagtaaaca
    34321 tataatgaca ggtatatcat atatgatacc agttttagta atgggtggac ttataggggc
    34381 cttatctcaa ttaataccat atgctatatt aggacttgac ccatctgtag gtatagttga
    34441 tgctatgaac tcaggagaat ttacagggtt caagcttagt cttttaaata tagcacaatt
    34501 aatgtcaaat ttcggattta ctttgtttgg atttgcgata ccactgtttg cagcattttg
    34561 tgcaaactct ataggtggaa aaacagcttt aattgcagga tttataggtg gatatatagc
    34621 taataaacct gtaggtgtac cacaattcgt tgatggacaa tggactgaag ttgttccagt
    34681 tgcatcagga tttttaggtg caatactaat agcatttata ataggatatt ttgtaaaatg
    34741 gttaaataaa tctataaaag tttctcataa ctggttagct tttaaaacaa catttttaat
    34801 accattaata gcgtcattag cttgtatggt attgatgata tttataataa ctccatttgg
    34861 tggattaatt aatgaaagta tgaaaaactt cttaacagca gcaggagcag ctggagaata
    34921 tgtatatgca acagctttgg cagcagctac agcattcgac ttaggaggac caataaataa
    34981 agcagcagga ttcgttgcac ttggtttaac tacagagaat gtacttccaa taacagcaag
    35041 aacaatagct atagttgttc ctccaattgg attagggtta acaacattat tagataaaag
    35101 attagtagga agaagagtat atgatagaca attctatcaa gcaggaaaaa cttctatatt
    35161 cttatcattt atgggtatat ctgaaggagc aataccattt gcacttgaga gaccaggttt
    35221 tgttatacct ttaaacatag ttggaagtgt aataggagct attacaggta taattctagg
    35281 agcaattcaa tggttcccag agtcagctat atgggcatgg cctctagtag ataacttatt
    35341 tggatatata ataggtatag cagttggtgc tatattcata gcagtaggaa atatattcta
    35401 tagaaataaa ttaataaaag atggtaaact tgttgtagat tatatcgact agtatttaat
    35461 ttttactatc tataaataac tttggctggt aatatggttt taatcaatat taccagcctt
    35521 acctatatga ttaattaaag gcggtggaga taagatatgg ataagataga aaaagtaaga
    35581 gagtatttta gagaatatga tatagatgga tttcttataa atagtcctac aaacaagttt
    35641 tatgttggaa atttatttag ctcttcaggt tatgttttta taacgaaaga atcacaatat
    35701 ataatagtag attttagata ttttgaagaa attaaaagaa aatctagttt gtttaatgta
    35761 gtattaatgg acaaaactag aactcatttt gatataatta atgatatttg tagagagcag
    35821 aatataaagg aaattggatt tgagggaaat gaagtatctt ttgatttata tagaagtatg
    35881 agtaataagt taagtgcaac cttaaaatca gttgatttat ctacattaag ggaaacaaaa
    35941 aatgaagacg aaataaaata tataaaaaaa gcttgtgaaa tagttgatgc tacattttat
    36001 catatagtag actttataaa ggttggtatg actgaaaagc aagttgaaaa tgaaattgtt
    36061 agaattataa aagatttagg tggtcaaaaa gaatctttcg atacaatagt tgcatctggg
    36121 cttagaggag cattacctca tggaaaagca agtgaaaaag ttatagagta tggagatttt
    36181 gttacctttg actttggtgc taagtataat aattattgtt cagatattac cagaaccatt
    36241 tgtatgggta ctataaataa agagttagaa gaaatatata atatagtaag aaaagctaat
    36301 gaagagtgta taagggtttt aagaccggga atgactactg gagagataga taaagttgca
    36361 agagacataa ttggttcata tggatatgct ggtaattttg gtcataatct gggtcatggt
    36421 gtaggaatta tggttcatga atatcctgca ttagctcctg aatcaaatga agtattaaaa
    36481 gaaggaatga ttgtaactat agagcctggt atatatgttc caagtcttgg aggcgtaaga
    36541 atagaagatg atgtacttat aacacaagat ggatgtatga gattgactac atctactaag
    36601 gatttaatag ttgtaagcta aaaataaatt agataaatat tttataacaa taagaaaaga
    36661 agaggtaaat ttaaatgtat aattttgatg ataaaccaga tagagtaagt gaaaagtgta
    36721 gaaaatggga tttaaatatt ataagagata aatttggaga tattagagaa gattttatac
    36781 caatgtggat tgctgatatg gattttaaaa taccaacaga aatagaaaac aaatttattg
    36841 aagctgtaag aagaggtgta tttggatata catactgtta tgatgaattt tatgattcag
    36901 ttattggttg gcaaaaaaat atgcatgagg taaatgtaga aaaagaatgg attacattaa
    36961 cttatggaac tgtatcaaca cttcattatg tagtacaagc tttttgtaag aaaggtgata
    37021 gtataatatt aaatacacca gtttatgacc cctttgaatc atcagcaaaa aaacaaggtg
    37081 taaatgtaat ttgtaataca ttagatgttg taaatgatag atattatata aattttgata
    37141 aattagaagt tcaaattaaa gaaaataagc ctaaattaat gatgttttgt acaccacata
    37201 atccatctgg aaggatatgg acaattgaag aaatgacaag ggtagcaact atatgtaaag
    37261 aaaataatgt tattttggtt gcagatgaag tacatgcaga gcatattcat tatggtacat
    37321 ttaattcaat acttaagatt ggaaaagagc ttttagaaaa tgttattcta ttgacatcac
    37381 caaataaagg atttaatctt ggtggattaa agacttctta ttcaatagtt ataaataagg
    37441 atataaggga caaatttaga aataaattaa agcaaaattc tataacttct ccaaatgtgt
    37501 ttggaataat aggattgata accgcttata atgaatgtca tgagtggtta agtggtgtaa
    37561 atgaatatat aaaatcaaat tatgaacttt tagaaacttg ggtaaataaa tataataaga
    37621 taaaattgat gaaaatggaa tcatcttact tagcttggat ggatataagt ggattaggta
    37681 ttagtgcaag tgagtttaca gataaattgg ctatagatac aggagtatta ttagaagatg
    37741 ggagtcattt tgtaaaagat ggtgagaagt atgttagaat aaatttagga actcaaaagg
    37801 aaaatgtaat tgaagcttta aatagaatgg atttattttt aaagtcttta taaaaatttg
    37861 gttgaattag tattttatat aaatttaaac tattaatatg gtatatacat aagtagaatt
    37921 tgttttagat tacttaagta tataccatat tttatataaa aattaaattt gcattagtca
    37981 tgtgataact atttaaattc atatgtattt tgtatgtata atagatataa ttattattta
    38041 aacttattgt ttatttaatt tttcaataga aaaagattta tctgtaagtt taaaattatc
    38101 ttttataccc ttagttaagt atactttctt atccttagtt atgaatatag catctacatt
    38161 atcaatttta ttaattaaat tcataccttt ttcaagacca agaccaaatg tagaagtgga
    38221 taaggcatca caattaatag atttatcaga gattattgta acactactta aattgttttc
    38281 aaaaggatac cctgtaaatg gatttaacat atgatgatat atttttccgt ttttttcgat
    38341 aaatctttca taaattccag aagtaacaac agacttattt gtagtttcta tgtttcctat
    38401 ggattcacca tttttactag ttgggtcttg aatacctatt ttaaaaggtt tatcattttt
    38461 accattttct aaagtaaata tatttcctcc taagtttact aaaccttttt ttacgtcctc
    38521 tgattttaaa taggctacaa ttttatcagc agcatatcct ttagctatgg cacctaaatc
    38581 aattttcata ttttctttgg ttaattttat agatttattt ttttcatcca atatgatgtt
    38641 cttataatca ataagaggca gaagtttttg tatctcagaa tcacttggaa ctttagcttt
    38701 atctgttcca atagcccata aatttgataa tggaccaatt gttatgtcaa aatatccatc
    38761 tgataatttg gaatattgta tagcttcttt tacgacgaaa aatgtatcat cagaaactct
    38821 gacaaatttt ttaccagcat tatcatttat tttagaaaca tcacttcctg gtatatgagt
    38881 gctcattttg ttgtcaatat atcttaaaat tgaatcacat tcatttaaga ttttatcact
    38941 tttggattct tttacattaa atacagtaac ttcgtttact gtaccaaggt agtagctagt
    39001 tttactatat ttagtattct ggcttgaact cctaaaaaat gaaaataaaa ttaatgctaa
    39061 aatcaatatc acaaaaataa atgtgatttt tttcttattc atggtatatt cctcctatga
    39121 aattgtgtta ttattttatt ataacataat ttcataggag gacaaaaaat gaagaaaaaa
    39181 gacattcttc ttattttagg cattttggtt gttattacag catgttatgg gataataaat
    39241 gttataaatt ctaaaaatgc tggaaatatt gagatttatg tagataataa attgtataaa
    39301 actgtttcaa taaatgccaa agaagaattt aaaatagaga atagaggggg gtataacata
    39361 gtaaagattc atgataaagg ggttgagata gtagatgctt catgtcctga taaggtttgc
    39421 gtacatactg gatttataaa caagccaagt caaagcatag tttgtatacc taatagagtg
    39481 agcataaaaa taaatacaaa tgaaaagaat gataatcaag aagatataat ttctaaataa
    39541 aataataaaa ataattgaaa ggaattggtg ataattatgg ttaacttagg aaacgattgg
    39601 gatgaattat tgaaagaaga atttgaaaaa gattactact taaacttaag aaaatttctt
    39661 atagaagaat ataaaacacg ccaaattttc ccaaatatgc acaatatata tgaagcttta
    39721 aaacacactt catacaaaga cacaaaagtc ttaatattag gacaagaccc ttatcatgga
    39781 gataatcaag ctcatggttt agctttttca gttcaaccac aagtgaaaac tccaccatct
    39841 ttattaaata tgtataaaga attaaaagat gatttaggtt gttttatacc taataatgga
    39901 tatttaatgc cttgggcaga ccaaggagtg ttacttttaa atactgctct tactgttaga
    39961 gcgcatgaag ctaattctca taagaacaaa ggatgggaaa ttttcactga tagagtaata
    40021 tctattttaa gtgagcgtga agacccagtt atttttgtac tatggggaag taatgcacgt
    40081 aaaaaagtag agttgattga tacaagtaaa cattatattt tggaagcacc tcatccaagc
    40141 ccattgtcag caagtaaagg cttttttgga tgtaaacact tctctaagat aaatgaaatt
    40201 ttgaaaaagc taggaaaaga acctattaat tggcagatag aaaatatata aaagtaaaag
    40261 gtaacaaaat gataacaaaa caacaataaa caatgacatt gttgtaacta atagaaatat
    40321 gcttaaatag ttgaaaatac tatgtatgca tatttctatt tttttgtcaa gttgacaact
    40381 cttgaaaaaa tagttacaat ttagttacac aaaaaacaca aataaaaaaa gaaacttaca
    40441 aagcagaatt ttatgaagca aaatatatag tgtaaaaaat cttagggagg aaattttata
    40501 tgaattttaa aaaattgagt aatgctaaag tctcggtaat ggcagttgtt ttatcagttg
    40561 gaatattatc gggatactct gcgcttaata aacaagtaac attagtagta aggggagaag
    40621 aaagaactat atcaacattt agttctaatg taggagacct tttagaatct caaaacatta
    40681 gctatgatgc aaatgatata gtaactttag atgaaaatac aaagttatct aatggagata
    40741 aaatagaagt aattgatgta aaggaaaaaa cagttgtaga aactaaagac attccatttg
    40801 aagttacagt tgttgaagat ggctctttat taaagggtga ttctaaggtt aaagaagaag
    40861 gaaagtctgg taaaagtgaa ttaatatata aagaaactta tcacaatgga aagaaagtag
    40921 aaaaggaatt tgtgaaaaag gttgttaaag ctgagccagt aaataaggtt gtaagcaaag
    40981 ggacaaaggt agaaatgcaa gtagcctcat caaggggaga aagttcaaga agaatggctt
    41041 catcatcatc tagtagcagt agtagtaaca atggtaaaaa tatgagagtg gtagcaactg
    41101 catatactgg tcacagtata actgcaacag gaacaaaacc taagtgggga acaatagctg
    41161 tagaccctaa ggttatacca tatggaacta aagtttatat acctcaattt gacatggtgt
    41221 ttactgcaga agactgtggt ggagcaataa aaggaaataa aattgatatc tacatgaatg
    41281 attcaaaaac agtttataac tggggaagaa aaactataga tatacgtata ttaaaatagt
    41341 taaataattt atatttttac tggaaattat attttgatta tatagagaag aaactcttta
    41401 tagattctaa aaatataatt tccagttttt tattttggat atgtaataaa acaggtaaat
    41461 tttaaccatg tatagataaa agctatttaa attataattt actatcatat aaatctataa
    41521 aaattgttgc aaagagtcaa aatatatgat attatagatt tcgttgagaa ggatttcatt
    41581 atgggagtgg atgggtgccg gtgtgccctc tagtcttcaa aactagtatg aggggttaag
    41641 agcctcttag gtgggttcga ttcccacgca ttcccgccaa tatgtaaaag gtatctttta
    41701 attaaaagat acctttttaa atttgtttta agataattta gttttaatga tatataatat
    41761 tttgtattat ataatgtaaa ttttataata ctattattgt tcagataaaa gtctaggaga
    41821 gtgaaaagat ggaaaataat tttacaacga gaactagttt tctagttgga gatgatggga
    41881 tagaaaaact taataattca aatataatag tatttggagt tggaggagtt ggtagtttta
    41941 ctgttgaagc tttagcaaga gctggagtag gcaatattac tattgtagat tttgatgatg
    42001 tagatataac caatataaat agacaaatac cagccctaca ttcaactgta ggaagataca
    42061 aagttgatgt catggaagaa agaatattag atataaatcc taatatcaat ataaaaaaaa
    42121 taagaagtct ttataataaa gatactagtg acgaaatact aactgaaaga tatgattatg
    42181 ttgtggatgc gatagacatg gtaagttcca aaatacatct aattgaaact tgtgaaaaaa
    42241 aaggattgaa aataataagt tctatgggta tgggaaataa attagaccca actaaaatag
    42301 tcgttacaga tatacacaaa acaagtactt gtcctctagc taaagttatg aggaaagaac
    42361 ttagggatag aggtatcaaa aagttaaaag tagtatattc tactgaacaa ccaatagaac
    42421 ttaagaagaa agtaatgaat ggtagaaaag taacacctgg tagtgtatca tttgtcccat
    42481 ctgtaggagg tttaataatc gcatctgtaa tagtaaatga actattagga caataagttt
    42541 taaatatttt aatttataaa ttttatatac aaatttgaag gctagaagga tgaatataaa
    42601 gcaattttat attcatactt ttagcctagt tattttatat taccttattt tatgtagttg
    42661 tagctttatt ttttagatta tgtatctatt tcataattat attttgtaaa tataatttct
    42721 ccatcactat aaaaaacaat atctcctatt ctgtctgttc tataaattga tacatggttt
    42781 tgttttagat tattaataac ctccctatgt ggatgaccat attgattttt atatccacat
    42841 gatattgcag ctatatctgg actaattttt tttataaatt ctaaagatga agaagaatta
    42901 cttccatgat gacctatttt tataaaatta atattattta atttaaaaga atgtaaaatt
    42961 tctttttcat tttcttcttc agcatcaccc ataaataaaa aagaattatc attaaaagtt
    43021 aatttgaaaa ctatagaatt taaattagat tcttcttgta tatatgatgg acttaataca
    43081 tatatattta tattgttatc tatgtttaaa acatcatttt tatagagatg ctgaatagat
    43141 aagtttttat ccgtacaaga atttattaga ttttgataag cctcactatc agttgattgt
    43201 tcaggcatat atatggagtt tacattaaat ttttttataa tattatcaag acttcctatg
    43261 tggtctgaat ctggatgagt tgctattata atatcaatag tttttattct tttttgtcta
    43321 agataactaa taattatatt ttcgctatct tcatcacctc catcaattaa tatattctta
    43381 tttgtgggtg tttgaactaa aatactatca ccttgtccaa catcaatcat atgtatagat
    43441 agtagagatt ttttgtcaca gccgattaaa agtgatatta taataagtat tgataaaaaa
    43501 attttatttc tcaataaaaa atcactccat tcattacatg tttatataaa tttatgatgg
    43561 gtaatataag atatatatga cttattatat tttatatttt aaacaatata aatgaaatta
    43621 ataccataat aaaattggag ttttttatat atacaaaata aaatttttaa aattaagcaa
    43681 taaaaaaggt aggaggattt tatgtttttc aaagatattg aactaaattc aaaaaaagag
    43741 ttagacccat attttgattt agtagattat gaagcttgtg agtactgttt tagtacttta
    43801 tatatgtggc aacatgttta taagacagga tattatatag gtgaagattt tgccgtttta
    43861 gttggggaat acgaaggaga tagcttctca atattaccac ttgctaaaaa agataaactt
    43921 ccagaagttg tagattttgt ccttgaatat ttttctaaaa ataataaaaa aatatactta
    43981 agaggtatta ctactgaagt agtagagttt ttaaaagaaa aatatccagg tagatttgag
    44041 tatatagaag aaagagattt atttgactat atttatgatg cagaaagttt aagaactcta
    44101 gctggtaaaa agaatcaaaa gaagagaaac catataaact atttcttaaa agagtatgca
    44161 ggtagatatg aagctaaatt attagataaa gaaaactttg atgagtgttt agtactaatg
    44221 aaggaatggg aaagtaataa ggaagaaaat aatgaatttg atgaaagtat ggatgatgaa
    44281 ttaataggta tcaaaaagat atttaaccat tatgacatat taaaagataa ggtaaaagtg
    44341 tttggtgttt atgtagatgg aaaattagaa gcttttagta taggagagct tttaaatcca
    44401 aatatggcat tgattcatat agaaaaagct aatcctgata taagaggtct gtatccattt
    44461 ataaatcaac aatttttggt aagtgaattt aaggacgttg agtttgtaaa tagagaagaa
    44521 gatttaggta ttgaaggatt aagaaaagct aaactttctt atcatccttg cagatttgtt
    44581 gaaaagtaca gtgttaggga ggcttaatag actatatgga gattaggtat gctaaagaag
    44641 aagagataga aagtataaaa gagatttgga gttattgttt taacgatacg gagagtttta
    44701 tgaagtacta ttttaatgat aaatataaat ctgaaaatac tgtagtagct ttagatgaag
    44761 gaaaaattat ttcttctctt cagctaaatc agtataagtt attactaaat agcaaggtat
    44821 ataatacatc atatgtagta ggagtttcta cacttccaga gggaagagga gctggatata
    44881 tgaacaaagt aatgaaattt actttaaacg aattgtataa aaaagggcaa ttagtttcta
    44941 tattgatgcc tattgactat agactttata gaagatttgg atatgagcat tgttacgacc
    45001 agattgaata tactattaac acagatgatt taaaaaattt taagtcaagt ggaaaaatga
    45061 taaagtctaa tttatcacaa attgatgatt tgatacaaat agatagaact tttttaaatg
    45121 aggttaatgg taatgtgtta aaagatgaac attattatga aaacttattt aaagagattc
    45181 aaagtgaaga tggattttta tacattcatg aaggaaatga aaaagatgga tatatagttt
    45241 attttcttca ggaagataaa atgtttgtca gagagttatt ttataaaaat atagatgccc
    45301 ttaaatcaat gcttaaattt atatataatc acaatactca atgtaagata gttactatat
    45361 ctactcctac aatagataag ataagattta tattagacaa ccctaaagat agtgatataa
    45421 aaataaaacc ttttatgatg ggaagagtta taaatgtaaa gaagtttatt gaagatatag
    45481 atattgagaa tgatattaat agctcattta atttattaat agaagacaag tttatagatg
    45541 aaaataatgg tctatttaaa atttctatac agaataagaa ggtatctgta gagcagttag
    45601 ataaaaaagg tgcagaaaag cctcaagaag attttgatat taaattagat ataaatacct
    45661 taacacagtt atcattttct tatatagatg taaatgaagc tattttctta aatgatataa
    45721 aagatgtttc agaagaaaca ctagaaacac ttaattgtat tttttctaaa aaaaataact
    45781 atataaatga atatatatag gtagagtacg ttaaaagatt ttaaaaatct tttgacgtat
    45841 ttttttgttt agataacaat ttatcttgtt taaaaatttt ctatttgaaa ataatctaaa
    45901 taatatactt ttatatggga gtgattataa ttcaaataag aagaccatta ttaattatat
    45961 taatgtttgt ggtagctata tcatttatca ttacaaataa cacaaaaaac gacgaagaac
    46021 ttaatgataa gataataact atacaaggag ttgtcaaagg aaaaattgaa aagaaaagat
    46081 acaatcagta caaggtaggg gtttttctaa taaatgatta tacaaaaaat aaaaatttga
    46141 agattggtca gaaagttaat attacaggta aattcaaaag tttagataaa atgaagtatg
    46201 atgattttga ttatggaagg tatataaaaa gtactggata taaaggtata gtgtatataa
    46261 ataactataa aattattgat aaaaataaac tttactcttt aataggggaa ataaagtttt
    46321 atattagtaa aacttatagg tacttatata agaaaaactc agattttata aattctatac
    46381 tattagcaga agttgaaaat ttgactgagg aacaaaaaga aatttttact agaactggaa
    46441 ctagtcatgt aatatctata tcaggtcttc atacaggaat attatgtgta attatatcct
    46501 ttctattaag aggtataaat aaattgtata aattattaat actgtcgatt tttataactt
    46561 tatattgtat aatggtaggt gcgtctccat ccataattag gtctatagca tttgttatga
    46621 ttttttattt atcaatattt attgatagaa agaaagatgg aatatcggca ttgtctctaa
    46681 ttgggataat tttaattatg aataacccct atgttatata taatgtaagt tttcaattgt
    46741 cttttttagc tacactttct atattaatat tctataataa aataaatagt attataaaat
    46801 taagtatggt atcattgaca atatcatcaa atatattaac tttacctata atttattata
    46861 catttaaagg tataccattg ctttcaatta taggaaattt aattatagta ccatttgtgg
    46921 gagtaataat gtatttaagt attgcaagtt taatcgtatt taaagtaagt gtagtaatag
    46981 ctaaaattat atcttttttt aatagtacat ttatagaaag catatttttt ttactagaaa
    47041 aaatcagcaa tttaagtttt gcatacatta atatagaaaa tcctaaattt tatattgttg
    47101 ttatttatta cataggagtg tttttttaca tattttacat agaaggaaaa gagataaagg
    47161 agcaagagaa tgaattacaa ggatattata aggaatgtaa aagagaaaaa ctttgaaaaa
    47221 atgtatcttt tttatggaag agagtattat ttaattgaga atgcaataaa agcatttaaa
    47281 gatagtctaa acgagggaat gcttgatttt aacttagata taatagatgg gaaggaaata
    47341 gtcttaaatc agcttataag ctctatagaa acacttccat ttatggatga tagaaagata
    47401 gtaataataa aagactttga actattaaaa ggaaagaaaa agaattttac agatagtgat
    47461 gagaaatact taatagagca tttagacaat attccagaca ccacaactat agtttttgtt
    47521 gtatatggag atgtagacaa aagaaaatca ttagttaaga aaataggaaa caatggaatt
    47581 gtttttgatt gtgataagct ttctgatatg gatttgttta aatggataaa aaaatctttt
    47641 gctttaaatg atgttataat agataattct caaatcatgt attttattga acaagaagga
    47701 tacagagata agagtagtga aaagacatta tctgatttag aaaatgaaat aaataaaata
    47761 agttcatttg ttggtaaagg gaataatgta accaatgatg taattgataa attatctcaa
    47821 aaaaaagttg agaatgatat attcaaatta atcgattata ttggagaaca aaatgcttct
    47881 aatgcaatga aaatattaaa tgatatgata caagaaggcg aatctgtatt aggtatattt
    47941 tctatgatag caagacaatt taaaataatt atgcaagtaa gacagttaca acttgatgga
    48001 tattcaacaa aacttatagc tgataaatta aaaatgcatc aatttgttgt aggaaaagca
    48061 ttaaagcaaa ctaaaaattt ttctgatgat ataattgtgg aaatattaaa ctatatatta
    48121 gaaagtgatt ataaaataaa gacaggtctt ataagagata ctttagcagt tgaaatgttg
    48181 gttagtagat actgtaaaag agaagcaatt taaaaaatct atatattaat aaaaaataaa
    48241 tagcaagata tgttatagtt cagagtagat gtaagctaaa ttaagtttga tatacaatca
    48301 actataatat gtctaactat ttatttttta ttgtataaat ttacaaaata aaaaacccct
    48361 acataggggt tattatatat aagaaatata aactggtaat taagcgttca taccgtttaa
    48421 tttttgagct aatttagcta cttttctagc tgcagcattc ttatgtatag ttcctttaga
    48481 agctacttgg tatattcttt tttgagcgta ttggaactta gcaacagcat cctctctgtt
    48541 tccagctgca acagcatctt cgaatcttct tattgcagtt tttatttgag attttctaac
    48601 tctatttaaa gcagttttct tttcgataac acttattctt ttcttagctg attttatatt
    48661 tgccaccgtg ttcacctccc tgtatttttt tatcatattc atcgtcaaca tattagattt
    48721 taacagaaat aaatctaaaa atcaaggatt attatttata tattttaata atgtttttta
    48781 ttttgatttt tt
//
LOCUS       DAMJYR010000017        46131 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.017, whole genome shotgun sequence.
ACCESSION   DAMJYR010000017 DAMJYR010000000
VERSION     DAMJYR010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 46131)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46131)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46131
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.017"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            164..3712
                     /locus_tag="PC471_000931"
     CDS             164..3712
                     /locus_tag="PC471_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HDF2788070.1"
                     /translation="MMNRLKPRILGISILFIIIIILTCGAVFKFKFKLDNKLKETTHT
                     YLSEFTEHNIINLKTRLSGQFDMLESIANFIGNLNNINDEMVIDLMDSDVKRSSLIRM
                     TISTLDGECYSNDGITHNVQDRDYFKKAKNGEKNISEPLKSIVNNKEIIVLSTPVYKS
                     GKIVGVLSGIYDSKKLNELLGLSAFDEKADVFVAKKNGDIVAQKTNKNRKTLSCIFQL
                     FDKDNCDEDDCLKTIKENMKNDKSGYFNYNLDNEKFMLNYQPLGVNDWYVFSVVPNKI
                     VSIQSENISYDVYIFALEILLVILIFIIYIACIINNNTKIIINEKEKFKVLTDNILGG
                     VKTCLKDEYMTMTYVSQGFLELTGYSCEDLNKIFKNRFKDMIYKDDLDKFNCIILNQL
                     EKQDKIEVEYRIVRKDGSIIWILERSKSINDNKGSECIQGVLTDITELKKIQEELDSK
                     RFEIQAINDSIVGGIVITEINDDFDSIYLNEGYLNMIGYTRKQLEDELNNKLIKLIFK
                     DDRTELINSINKQLKLSDTFASEQRVEKRDGTIIWILLKGRKMIYDNGKSVLCCVIVD
                     ITSAKLLEQELKKSEERHEIILKQTQNIIIEWDIEKDKMNISPNWYNKFKYDPITENA
                     TKNIIKSVDIYHDDMDKFISLIKLTSQGSEYEECEIRLRTRDTIYAWFSIKATTLFDN
                     NGKPYKVVGSLTDIDESKREAEKLKERAEKDPLTDLYNKKASQSLIEEYITLDKKRKG
                     ALMIIDIDDFKGINDNLGHLYGDAVLSEIANDLINLFRATDIVGRIGGDEFIVFIKDI
                     SETRDIINKAEELVKTFERSFLGKGRNYKISLSVGIAKFPQDGMDYMTLFRNADIALY
                     SAKGKGKNCYTLYDKKFDKVQYITNTRNAGDSDNVEMSFKEHITNYIFDILYESSDIE
                     VAVNLILSIVGKHFNVSRMYIFENSDDNRVCKNTFEWCNEGVTSEINNLKNICYKDLG
                     NYLLNFNADGLFYCNDIENLGSDVKKVLKPQGIRSILQCKILEGNEFRGFIGFDECSY
                     NRMWTNDEVNTLTFISKTLSMFLLKMRIQSKLEESLTIQKSVMDNMDIWAYVVDRNTY
                     ELLFINKKMLESAPNTHVGDLCYKAIWNNQDKPCKKCPMIYLKDGDDTYKSFMHNKKF
                     EVQANITTTTLKWFDGKDACLIFGSEITSIEAEENT"
     gene            3897..5831
                     /locus_tag="PC471_000932"
     CDS             3897..5831
                     /locus_tag="PC471_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HDF2788071.1"
                     /translation="MENKFLPISKQDMIDRGWEELDFVLVTGDAYVDHHSFGTAIISR
                     VLESAGYKVGIIAQPDWRTTDDFMKLGKPRLAFLVNAGNMDSMVNHYSVSKKHREKDM
                     YSPGGKMGYRPDRATIVYCNKIREAYSDVAIVIGGIEASLRRFAHYDYWSDKVRKSML
                     IDSGADLLVYGMSEKQIVEVANALNDGYDPKYIRHIDGTCYISDTLEEIYDKYILIPS
                     YKEICEDKMKYVEAFKIQYDEQDPFRGNIIVQPHGSKYLVQNKPEKPLSREELDEVYG
                     LPYQKTYHPVYEKFGGIPAIEEVKFSIVSSRGCFGSCSFCAITFHQGRAVQSRSEKSI
                     IDEAIGITNLDDFKGYIHDVGGPTANFRRPACKKQITKGACKNRQCLSPSPCKNLDAD
                     HSEYLHLLRAVRKLPKIKKVFVRSGIRYDYVMADKNNKFLRELIEHHVSGQLKVAPEH
                     ISEEVLKYMQKPAGKTYDKFRQKFFAINEELGKKQYLIPYLMSSHPGSTLNSAIELAE
                     YLRDTHYQPEQVQDFYPTPGTLSTTMFYTGIDPLTMKPVYVPKSKRDKAMQRALLQYR
                     APRNYDLVYSALVEAGREDLIGFGHRCLIKPKNEKPYFNRNNSKKNVSKGTNKNKKTN
                     TNNRSNKNQQKSSTKKKKKH"
     gene            5937..6932
                     /locus_tag="PC471_000933"
     CDS             5937..6932
                     /locus_tag="PC471_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDF2788072.1"
                     /translation="MKKYISIILLVVLTMVVLVGCSPGKDNPKNKELSVVKQVKVAVP
                     DGLPAVAIAKLANENPEIKEGYETVYSIEKTPEAISTRVMKKDADIAIVPSNMAAIAY
                     NKTSSYNIVGTVGMGSLYLVSTENIKDYSDLVGKEVGCTGKGLTPDITIKSLLLQKDI
                     NYSDIKFNYVNSASELVPLLATGKVKTGIVPEPALSALMSKNPDIKIIKSLNDSWKEV
                     SGSKDGYPQSTLIVKSEFLRDNKDFVDSFVGQLSNSIDWANKNPEELGAYSEKIKIST
                     ESKIIGKSLERANLKYIPVKDMIKDYKNYYEKLANFDDKTLGGKVPDEAIYFVEK"
     gene            6904..7689
                     /locus_tag="PC471_000934"
     CDS             6904..7689
                     /locus_tag="PC471_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HDF2788073.1"
                     /translation="MKQYTLLRNKKNKEQFIDKMEVTLSCVILLFLWQIIALKIDNDI
                     FLPTVGQVFNSIKEIMLNSSFYIDILYSMGRCFFSFLLAMIFAIILSIVSYLNRFFRN
                     LLKPINALTRSIPTMILVVLALIWFEKDSTPFIVGFTIVFPILYDNVLGAILNIDKNL
                     LEMANVYKIRFIDKVLKIYLPAIKFQIISILVSTFSLSLKVAIAGEVYSQPTYGIGTM
                     IQTEKINFNTSGIFAWIIIVLLISAILQIAQKFLARRAFLWKR"
     gene            7677..8375
                     /locus_tag="PC471_000935"
     CDS             7677..8375
                     /locus_tag="PC471_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2788074.1"
                     /translation="MEKIKLSIENINKRYDSRIIFRDFNIDFYVNEVNCILGKSGCGK
                     TTLLNIISGIIKNDTNNLNIKENLNRVGNKLEASYIFQDDRLIDWLTVEENIKIVVNK
                     YYNKTQLNKICDEYLELVGISDYKKFYPQMLSGGIRQRVNIARAFIYPSKNIIMDEPF
                     KSIDAKNTQLIMDNFRNILRKEKRTVLFVTHNIEEALFLADRIFILGDSPIRIKKILK
                     NSKELEKNEVLKLI"
     gene            8498..8971
                     /gene="ybaK"
                     /locus_tag="PC471_000936"
     CDS             8498..8971
                     /gene="ybaK"
                     /locus_tag="PC471_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HDF2788075.1"
                     /translation="MVKTNAMRILDSNKIDYKVMSYEVKSEHVDGVEVAHDIGRDVNE
                     VYKTLVTQGVSKNIYVYVIPVHENLDLKKAAKVAKEKSVEMIHVKDINKLTGYIRGGC
                     SPIGMKKFYKTFVNESAKNLDTIIVSAGKIGYQIELSPFDLQRLIKVEFVDVIKK"
     gene            9168..10007
                     /locus_tag="PC471_000937"
     CDS             9168..10007
                     /locus_tag="PC471_000937"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HDF2788076.1"
                     /translation="MRKIKSEQIVEQVKKLCIEASLYLGEDVLSCIKEKAKSEKSEVG
                     KNILNILVENAEIAKEKNIPICQDTGMAVFFVEIGQEVLIEGDTLTDAINEGVRQGYE
                     EGYLRKSVVSPINRVNTKDNTPAVIHYDMVKGDKIKIEFAAKGFGSENMSKMKMLKPS
                     DGLEGIKKFIIDTVSEAGPNPCPPMVIGVGIGGTVDKCAQIAKKALFRELGEFNKDEN
                     IAKLESELLTAINKLGIGPQGLGGTTTALGLNIETFPTHIAGLPVVVNINCHASRHKN
                     VVI"
     gene            10020..10568
                     /locus_tag="PC471_000938"
     CDS             10020..10568
                     /locus_tag="PC471_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S-containing hydro-lyase"
                     /protein_id="HDF2788077.1"
                     /translation="MIKITTPVNEIDIAKLNCGDTISLSGILYTARDAAHKRLIDCIN
                     KGEELPFDVYGQGIYYVGPTPTKPGEVIGAAGPTTSYRMDDLTIPLLERGLRLMIGKG
                     KRSDEVIEGMQKYGAVYLAAIGGAGAYISNSIKSCEIIAYEDLGAEAIRKIVVEDLKL
                     TVAIDSYGNNIYEQGRAIYECK"
     gene            10634..11815
                     /locus_tag="PC471_000939"
     CDS             10634..11815
                     /locus_tag="PC471_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HDF2788078.1"
                     /translation="MSKDYAKLALEMHEINKGKVSVESKVEIKTKDDLSTAYTPGVAE
                     PCLKIHENQDDVYRYTSKGNLVAVVSDGSAVLGLGNIGAEASIPVMEGKAILFKQFAD
                     VDAFPICLKTNDVDEIVKSVELMEPVFGGINLEDISSPRCFEIEERLKKSLSIPVFHD
                     DQHGTAIIVAAAIINSIKLIENKKIEDLEIVINGAGAAGIAIAKILLNMNVKNIILCD
                     RSGALEASIENLNYVQKEMLKVTNIRNEKGPLKDIIKGKDVFIGVSGPGAVTKEMVES
                     MSEKPIILAMANPTPEIMPEEAKLGGAFIMGTGRSDFPNQVNNVLAFPGIFRGALDVK
                     AKEINEEMKIAAAYAIANTISDEEICPEYILPDVFNKNVVKNVAKAVKEAAIKTKVNR
                     I"
     gene            12110..12496
                     /locus_tag="PC471_000940"
     CDS             12110..12496
                     /locus_tag="PC471_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009248914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDF2788079.1"
                     /translation="MEYKVNDQELNASMFIPFVNRIWPGDYDMDKTQTALSKTLNITA
                     YDDKVLVGCLRVLSDGYYFGTITELLVLPEYQKQGIGSKLFQLAKENTPTMLYFGAQP
                     GVEAFYEKNGCQRSLQSYIIKKKDSY"
     gene            12646..12909
                     /locus_tag="PC471_000941"
     CDS             12646..12909
                     /locus_tag="PC471_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HDF2788080.1"
                     /translation="MEETKFCQCCAMPMGQTDELYGTNKDGSKSTDYCSYCFKDGEFT
                     ADISMEEMIEVCIPHMLQSNKDMTEDEARTMMNNFFPTLKRWK"
     gene            complement(13038..13880)
                     /locus_tag="PC471_000942"
     CDS             complement(13038..13880)
                     /locus_tag="PC471_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HDF2788081.1"
                     /translation="MINIGDFNKLTVKRKTEFGYFLDGQTNNTKDDILLHNRLIGKNE
                     INIGDEVNAFIFKDSDDRTAATLIPPLAKVGDVAHLKVVDNTDIGTFIDMGLPKDILV
                     PFKAKTYPLFRDEKYLFYIYLDKSKRIAATTDIDSYLLTDHTYNVGDIVTGVVYGFQT
                     NNSAMICVDNKYAGVILHNEYFTELKTGDVLENLHVIKIYEDGKLGLSPRGNRKDELD
                     TLENKILSYLEGSDGYMRFNDKSDPKDISILFNSSKKNFKRALGVLMKKGLIYQDEEG
                     TYLK"
     gene            14175..14909
                     /locus_tag="PC471_000943"
     CDS             14175..14909
                     /locus_tag="PC471_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDF2788082.1"
                     /translation="MKIVNKSSNIPLHTQLSSIIREMIETGELKEGDAIMPERELCNI
                     QNVSRMTVNKTIVGLVTEGLLYRVQGKGTFVAKQKKKYQFSNVKGFTDVMKEKGVNIK
                     TDILSFEMELPDDLVKRKLGISDNTTNIYKIVRLRYTDGEPFGLEIVYLSEEMCKGLT
                     KGILDNSSLYRVLNEKYGYKIQKAEQVMEPVILSDEESKLLETDEGALALKLHRNSYN
                     REGSPIEYTISIFRTDKYQYEIVLSE"
     gene            14919..16052
                     /gene="nagA"
                     /locus_tag="PC471_000944"
     CDS             14919..16052
                     /gene="nagA"
                     /locus_tag="PC471_000944"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HDF2788083.1"
                     /translation="MKCLINGKIILKNQILENKVLVFDEKIIDIADSVPKDCEVIDAD
                     GKYISPGLIDIHIHGNMGKDTMDSTDESIETISKSIMRHGVTSFLPTTMTMDKEHVYD
                     ALEVIKKAQNRKLEGAQVLGAHLEGPFINENYKGAQNEKFIINSKYEFIKEYKDVIKV
                     ITYAPEKDIDFDFTREIKRCTDIVLSIGHSNANYDQAKEAINLGVTNVTHMFNAMTGL
                     NHRDPGVVGAALTTNVYSELIADTIHINKDLFQFILNNKGKERLILITDSIEAGGLED
                     GNYSLGGQKVIVKGNEARLENGALAGSVLSLNKMVFNFLDNTNLKVNEAINLASLNPA
                     TSLGINDKKGSLEIGKDADIAVFDENLDCKMTLCLGDVVYKNI"
     gene            16151..16900
                     /gene="nagB"
                     /locus_tag="PC471_000945"
     CDS             16151..16900
                     /gene="nagB"
                     /locus_tag="PC471_000945"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012425552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HDF2788084.1"
                     /translation="MRVLVCKDYDGMSKKAAEMIAAQIVLKPNSILGLATGSTPVGMY
                     RDLVKKYNDNIVDFSDVMSFNLDEYYKLPISNDQSYDYFMKENLFNHVNIKPENTHLP
                     NGMADDIEKECMNYEASIDAAGGIDVQVLGIGRNAHIGFNEPDTKFAKRTHVVELTES
                     TIEANARFFKSREDVPKKAVSMGIGSILKSKKILLLASGEEKADAVYNTVYGDITPEV
                     PGSILQLHKDTIVIVDEAAASKLNPKDYKLV"
     gene            17075..17347
                     /locus_tag="PC471_000946"
     CDS             17075..17347
                     /locus_tag="PC471_000946"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2788085.1"
                     /translation="MLKIIMFSGILSSIIKLPIIGILPTGNIKKNTGISDSKKDGKQR
                     RSNLGISIYQLPSFLYSLINNSVPSNKPIPPEEIKLIKMYLLKSCI"
     gene            18007..18711
                     /locus_tag="PC471_000947"
     CDS             18007..18711
                     /locus_tag="PC471_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2788086.1"
                     /translation="MSKLIYIADDEDNIRNLVKTFLKNEGHDVMDFKTGDELLEQFNI
                     KECDLVILDIMMPGSSGFEVCTKLREKSTVPIIMLTARDTDIDYITGITLGSDDYFTK
                     PFSPMSLVMRVKSIFRRIEFEKKQNYDKYSNSIDMELKFGDVIINKKNKIVTSKNVNI
                     DLTPNEYNLLTYLFENIDRAVSRDELLNKIWGYDIEVETRAADDTVKRLRKKILNTNI
                     LIETVWGFGFRLKEKS"
     gene            18713..20200
                     /locus_tag="PC471_000948"
     CDS             18713..20200
                     /locus_tag="PC471_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF2788087.1"
                     /translation="MKNGNDKNRIGKIKSKFKLKFSKKNISIKTRILLSILCLIIIVF
                     MIILVSFNVFLDTYIKTTANEELTKSTEIVEHMDSNFRPIKPPQDDGKLPPKVLSNFM
                     RNVQDKVRMAETQSDADAMVVDSKYNLIFPTREDDFLKNVDEMELIRECIQNEKLDLN
                     SDENTRISTRNKDYYISTVKITSIYGEQDKYLILFIDISKTLNLAQKINIVLISVMCF
                     AGILAIFTAVILSEKIAKPIKELCEFAKKMGQGDFKRTYFDFSDKELVELSTVMNKSA
                     EYLDKYDNEQKIFFQNASHELRTPLMSIKGYAEAIKYNVIDSKHASDIILEESDRLSD
                     MVEDLLYISKIDNITKDFELVECDLREVLSNCGARQNVRAINKGIKFIYEFDEEMVLF
                     ECDEKNISKAFMNLIENALRYAKSEIKIVCKYNQKNIVVIIEDDGIGIKKEDLPHVFE
                     RFYKGVGGNHGIGLSIVKSIVNKHGGRIYVENGKKGAKFTILFKL"
     gene            20405..22018
                     /locus_tag="PC471_000949"
     CDS             20405..22018
                     /locus_tag="PC471_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HDF2788088.1"
                     /translation="MKEQLIFNITNLVTVVFEAFVIHMFLSDFLGQKEGYIKVVRYAK
                     LGFIICLGFCNLITLNPKITMPLIFILIFSTSFLYKGNLKTRLFTTVLLSIFFILSEI
                     VVTSIFVLFVKEGFEIMLENNSIRVLATILSKIVFLLTCKIICLFKKDVHLDMPIKYW
                     LPLFLIPIFSLFLSVSIFDVSKFFSLESLKFLSLISSVGILYINFIVFYLFKFIIDKT
                     KLSMKYELLEKEIIHKEELRLSNECYKIIVEQTDSVVFEWNIKENKSFVSQAWTEKFG
                     YNNACKNIFKEIKDKDLVHSEDKAIFEGFLESIKKKNMHNQAVYRLKKSNGEYIWCRT
                     SITSIYNDENELLRVVGVIVDVDSDIKKYEELRTRAESDSLTNIYNKGTFEKLVEETI
                     VMNTGDKKDALFIIDLDDFKEINDNFGHPFGDFVLKTFADKIQTSFGSKDLVGRIGGD
                     EFVVYMQDYVTEVNLHKKAKELNRVLSDNYTDLSFSFDASVSIGIARYPQDGTSFFEL
                     FKNADRALYSIKASGKNSYCLFEEELYVQ"
     gene            complement(22381..23211)
                     /locus_tag="PC471_000950"
     CDS             complement(22381..23211)
                     /locus_tag="PC471_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDF2788089.1"
                     /translation="MSQVSKNYFTTGEFAKICGINKKTLFHYDDIGLFSPELKKENGY
                     RYYSYHQLSIFGIISSLREVKMPLKEIKAYIDKRTPNLLIELLEKKTIDIKNEIEKLN
                     NIQALMESTISFTKSACNIDANTITLKEHEEEYLVKTPVTYKEQFLGDEEENFLYECI
                     NFMDNYELSDYGTIGSIIKGEDIINKDFESYSYLFTKVNKEYKKYPVSIKPKGLYVTA
                     YHKGSYETIYKAYEKLLNFFNQNNLRIGDFVYEEYLLYDISVRDSNEYLTQISAEVKM
                     "
     gene            23284..25011
                     /locus_tag="PC471_000951"
     CDS             23284..25011
                     /locus_tag="PC471_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2788090.1"
                     /translation="MKKLIHFLKPYRVLIVVVLIFTFLQTLGTLYIPTLTANIVNNGV
                     VKGDIDYIVKTGLMMMIVAGITALSAVLVCKVSANLSSGFCRDIREAVFIKSQDLSIN
                     DFNNIGTASMITRSTSDITLIGQSVFMFIQLVLPAPIITVSGLFLAYSIDKAMTIIIV
                     VVMFLFMLSAFLVGKKLIKLFKMMQIKMDNMNRVLREVVTGVRVIRAFNRSRFEKKRF
                     DRTAIDYSETAISINKIFAVLMPIVMLIMNLGIVSIIWFGGMRVSNGNMEIGHIMALV
                     EYCILILFYLIMGVMVFMYIPRAGACADRVNQILDIEPEIVDGNGHKDTVSERGHLVF
                     KNVTFSYAQSEEPVLNNITFEAKSGEVTAIIGSTGSGKSTIANIIPRFFEIQSGEISI
                     NGQDIKKIPQKELRDKIGFVPQKAFLFSGTIEENIRYGKEDASIEEVKHAASIAQADE
                     FIYDMEDKYDSFVAQGGNNLSGGQKQRISIARALVRKPEVYVFDDSFSALDFKTDKRL
                     RKALKNEIKDSSAIIIAQRISTIMDANQIIVLNDGKIVGIGKHKDLLENCEVYKQIAD
                     SQLSKEELA"
     gene            25048..26910
                     /locus_tag="PC471_000952"
     CDS             25048..26910
                     /locus_tag="PC471_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2788091.1"
                     /translation="MSEKRIDEQDIEVLSFEDGMSAEKAKDAKKTTKRLLKYMAKQKF
                     KILIIFISVLISSALTVLAPMVMGKAIDQLFNGIKTAVQTGTKFSVNFSTMGGIVSIL
                     LGLYLISAVFIYIQQYIMSGVAQNLVLSMRKDLSDKLNKLPLKYYDSHKKGETLSIVT
                     NDLEKVADSLQEGLMQLITAVVTVVGSIVMMISISVPLTIVSAITLLVSLGITVVIAR
                     KSQERFSENQKALGELNSNIEEIFTGQIVVKAFSKEKDTIRNFKILNQNLYNASRKAQ
                     FSSYAISPIIRFINLIGYVIIAVVGGIFASTGAMTLGSIQAFIQYVNQASEPTTEISY
                     IVNMLQAAIASAERVFTVMDEVEEIKDEESSKVISMPKGKVQFEHVKFGYSDDFILMK
                     DININLNAGDKIAIVGPTGAGKTTLVNLLMRFYEIQGGRITIDGVNIKDLKRGELRTM
                     FGMVLQDTWLFNGSIKENIAYSKSDATMDEIVRAAKSARVDHFIRTLPQGYDTILTED
                     ASNLSQGQKQLLTIARAILSDPSVLILDEATSSVDTRTEVEIQKAMNNLMKGRTSFVI
                     AHRLSTIRDADLILVMKEGTIIEQGSHKELIEKKGFYEELYNSQFTSEYDEDVV"
     gene            27243..27932
                     /locus_tag="PC471_000953"
     CDS             27243..27932
                     /locus_tag="PC471_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate polymerase domain-containing
                     protein"
                     /protein_id="HDF2788092.1"
                     /translation="MNKKLNYRFEMKHKITEADVLALKSRLYPIMKKDENASKDGKYL
                     IRSLYFDTPEDKALLDKLNGVAIREKFRIRFYNNDCSYIRLEKKIKHYNMTSKLSTNL
                     TKKEVINILNNDIEFLKESTNCLLREFYLKLKCERLEAKSIVDYNREAFIYPVGNVRV
                     TIDSDIKTSVNSVDLFNKDLPTISVIDENMTVLEVKYDEFIPDFIKDLIQINKTTSTA
                     VSKYASSRLYI"
     gene            28037..28723
                     /locus_tag="PC471_000954"
     CDS             28037..28723
                     /locus_tag="PC471_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4956 domain-containing protein"
                     /protein_id="HDF2788093.1"
                     /translation="MILIMTFNDIFKSSFIENVSGFSFVDSALALGSAFLVGLFIYMV
                     YKKTYMGIMYSRPFNVSLVALTMLTTFVILAVTSNVVLSLGMVGALSIVRFRTAIKDP
                     MDLVFLFWSLGSGIVLGAGLIPLAVMGSIIMGLILIFFSNKTISETPYILMVNCNNED
                     SEDIATDKIKKVFTKYQIKSKSVTPEKGIELVFEVRMKDGETSLINDLSQVDGVTNAV
                     LVSYNGDYVA"
     gene            28740..30647
                     /locus_tag="PC471_000955"
     CDS             28740..30647
                     /locus_tag="PC471_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HDF2788094.1"
                     /translation="MKDKKFTLLISIMIIFLCAVVGVYSTSSNKSADLYSDVYIEKYF
                     NRDKVMEVNIEIDESDLKDMNENAIKEEFKVAKVTVDGDTYGNVGIRTKGNSSLISVA
                     NSDSDRYSYKINFDKYNTSQSMEGLTQLNLNNCYSDPSYMREFLTYSICEEMGLATPE
                     FAYAKVSINGEYHGLYLAVEGLKESYLENNFGNVTGDLYKSDEGSSLQYKGDDPESYS
                     NLIVESDKKTADWSKITKLLKSLDTGEDIEKYLDVDSVLKNIAINTALLNLDSYQGSF
                     AHNYYLYEQDGVFSMLPWDFNMSFGGFSGFGGGSQSIAIDEPTTGNLEDRPLISSLLK
                     NETYKTKYHKYLEEIVTKYLDSDYLENMTTKLHDMIASYVKEDPTAFYTYEEFEKNIT
                     SSIEDSSDNKGFGNKGFDNNNSNNSDSNNNSNSENKRSGNQSDEKEVNAELTSSVVKA
                     NTDNETKNKTTNDSESKNNTDKDKSGNDNNQKLEGPMGKGGKSIPGVLEVAEDMSKTI
                     KSQLSGETSSTKQNSGDESSSGIKGSEKFDEDMSGMPEPPEGMDGKMPPGMGNMDKGD
                     MNGKNGNMNMDRNQDNPREAGGFGNRGGGSVSKTTTYFKLILGGASMIIMSIMLVGVS
                     RVKRRRFIKSK"
     gene            31100..31789
                     /locus_tag="PC471_000956"
     CDS             31100..31789
                     /locus_tag="PC471_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2788095.1"
                     /translation="MLQKVLWKFLKLVVGLFICSVGIVLTINCNLGLSPWDVFHQGLS
                     NHIGITIGTASIIVGSIVVIADVVLGENVGWGTVFNMLLIGFFMDLLLYSNLIPEADS
                     LFVGIIMLILGLVLLSVGMVFYMGSGLGSGPRDGLMVAIQKKTGKSLKLIRGTIEVGA
                     LIVGFLLGGKVGIGTIISAFGLGYFTQMVFGLFKLDCSKIKHRFIVDDIKFIKVYMSG
                     EKKAAVQNIVK"
     gene            32225..32764
                     /locus_tag="PC471_000957"
     CDS             32225..32764
                     /locus_tag="PC471_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007286148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2788096.1"
                     /translation="MDIGEKIKRLRTEKQLTQEELANRCELSKGFISQLENNLTSPSI
                     ATLIDILEILGTNLREFFNEIDDERISFTKEDMFETEDEDLKYKLKWLIPNSQKNEME
                     PIIITLYPGGQYKEEKPHEGEEFGYVLAGSIYVHIGEKKNKVKKGESFYFRPKANHYI
                     SNEGKTTAKVIWVSTPPSF"
     gene            32783..33826
                     /locus_tag="PC471_000958"
     CDS             32783..33826
                     /locus_tag="PC471_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011721317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2788097.1"
                     /translation="MVENIIELRDISKHYEELTVLDNFNLDIKKNEFLTLLGPSGCGK
                     TTTLKIIAGFEYADDGKVLFEGKEINNLPPYERQVNTVFQKYALFPHMDVYENVAFGL
                     KIKKTPKDVIDAKVKEVLKLVALEGFERRQVESLSGGQQQRIAIARALVNEPKVLLLD
                     EPLGALDLKLRQEMQIELKRIQKKLGITFVFVTHDQEEALTMSDTIVVMYKGKIQQMG
                     TPQDIYNEPKNSFVAKFIGESNIFDGIMIEDYKVNFCNRDFECVDKGFEKNENIEVVI
                     RPEDIKMVKPEEGMLKGKVTSTVFKGVHYEIELNENGRLWILHNTKNAEVGTELGMDI
                     YPEDIHIMRKENN"
     gene            33828..34664
                     /locus_tag="PC471_000959"
     CDS             33828..34664
                     /locus_tag="PC471_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2788098.1"
                     /translation="MKRKSFLAYPYVVWSAIFVIIPLILVVFFSFTKESGGGYAFTLE
                     NYKEVIDPIYMKVFGRSILLAGGATLICLIVGYPVAYIISKARVSRRGSLILLFILPM
                     WMNFLLRTYAWVAILGKNGLLNTFLGWFGIQPLAILYTNFAILLGMVYNFLPFMVLPI
                     YTALSKMDNDLINAAHDLGANNMTVFRKIIFPLSLPGVMSGITMVFMPAVTTFAISRL
                     LGGGKIMLVGDLIEQQFTVVGDWNFGSAISIFMMIVILISMSIMSKFGDESDKEGGGL
                     LF"
     gene            34769..35443
                     /locus_tag="PC471_000960"
     CDS             34769..35443
                     /locus_tag="PC471_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2788099.1"
                     /translation="MARWNGFTWKWYGQLLQNESIMSALYYTIVIAILASVISTIVGT
                     ISAIGIHKMRGKSKKLILNVNYLPILNTEIVTAVALMSLFVFVKMEFGFTTMLLAHIM
                     FCLPYVILSVLPKMKQLPDNIEDAAMDLGATPIYALRKVILPQIKPGIVSGFLIAFTM
                     SIDDFIISFFNAGNGVSNLSIEIYGMARRGIKPEINALSTIMFAVVLGLLLLANKKES
                     IVRGIK"
     gene            35443..36495
                     /locus_tag="PC471_000961"
     CDS             35443..36495
                     /locus_tag="PC471_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein"
                     /protein_id="HDF2788100.1"
                     /translation="MIKFKKAISLIAVGMMTVTLFTGCNKATDSTKVLNVYNVGDYID
                     ESLIDKFEKETGIDVQYSTYDTNEMMYQKVKSGSTHYDLVFPSDYMVEKMKNEGLLEK
                     LDFKNIPNMKYLDKSFLNPVYDKTNEYSVAYMWGTLGILYNKKEVKDPMDSWNILWNP
                     KYKGNIMMFDSVRDTIGITLKKLGYSMNSVNPKEINEAKNLLMKQKDLVLAYVNDEGK
                     DRLLGGEVAMGMLYSGDAVTLMEQNPDLDYAIPKEGTNKWVDAMCVPTTAQNKKEAEL
                     FINFLLDPENAKVNAEYIGYSTPNTGALKLLDPEITQNPVAYPSKEVLDKCETFEDIG
                     ENIKLYDKAWIELKSK"
     gene            36725..37573
                     /locus_tag="PC471_000962"
     CDS             36725..37573
                     /locus_tag="PC471_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HDF2788101.1"
                     /translation="MNRINKKIDLLILALLVFIFLIVGIVILSIRTENKLNVFIMLAI
                     IFFIIMLTYLSNSVVGLITSSIIIFMYTSYILYNNITHNMDVEFISYMWIIATPVSSI
                     IMGNLNKSINELQNTNKKLSEQYKELVTIDSETGLRNLKIFYNDVNMEISKSIRHNTD
                     FSLMIVKLPYYGNLQTIFGENKTNKIVKYIGSNIIECTRNEDIIYSLQKDMIGILMPN
                     TSLEGSKVVKDRIKKRIKELNLDLNNRGKYVNIDVKIAFLQYKSSFGDSINFKNIVEE
                     ELQYDV"
     gene            37563..39227
                     /locus_tag="PC471_000963"
     CDS             37563..39227
                     /locus_tag="PC471_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HDF2788102.1"
                     /translation="MMFKIFNKSLLKLVVVLSIVLTISNFSLSSYASEITSSTDKVLI
                     IYDSKKETAYNRDILNIMRTLLGRFSSDIELLKLSNYDGEINKNYYSHIFILGINENS
                     YNNDKNTKNLISSLNLYKGTICWLGYGIENLLEHKKYNLDYVGKTNNIVSVNYRGKSY
                     NLDEHYVFNIVESKDTSNKVIGSINDTLNKYPYIINDKNLFYVSKLDLDGVLFYIFCD
                     SLNDIFNIKTFDKGRIFVRIEDVHAFREPKNLVEIADYLSSKNIPFTIALIPAYVNPK
                     NHKVITLSESPEIVKAIKYMQDKGGTVILHGYTHQYKKEEVSGEGYEFWDGKKDEPLK
                     ENMKIFVKDRVLNGLRVCIENGIYPLAFEAPHYAMESEGYKELKKYFSTYIGQHQNND
                     KKFSTNTYPYIIRDTEEFNIFIPENLGYIDPEDKFTFQNIKENLDKLSIVRGFSGGFF
                     FHSYLNIEYLKNTIEYLEKQNIEFMNLRDFNNWVKVDEIQIRNNGDEIIVNYDKDLDE
                     LTKSDTRFKSISNISKVLIFIVSISVLIFVIIFIYFKRIDKKKFLK"
     gene            39276..40532
                     /locus_tag="PC471_000964"
     CDS             39276..40532
                     /locus_tag="PC471_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003362983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HDF2788103.1"
                     /translation="MRDLNVSEYLFVFSLFSIWSLLLINIILAMGGYIFYFKNFDKEI
                     KEIDEYPMISILVPAHNEAKVIGRTVESLLLLNYPKSKMELIVINDNSSDNSKEILEN
                     IKDRYNNYNFTIINTDSLTGGKGKSNALNIGYTISKGDFIAVYDADNTPDKNALRYLV
                     QTIVMNDELGAVIGKFRTRNKNKNLLTKFINIETLSFQWMSQAGRWQLFNLCTIPGTN
                     FILRRSIIEEIGGWDSKAIAEDTEISFRIYKLGYKIKLVPQSITWEQEPETVKVWIKQ
                     RTRWAKGNIYVLMKYIKNIFKQGRNKIVFDIAYFFSVYFLFLTSVIISDILFVLSISK
                     LVEISIPINFFLIWILSYLLFIIEVSISLTIEKGEATIENIFIVAIMYFTYSQLWLFV
                     AIKGMIEYLKDIIFKREVKWYKTERF"
     gene            40555..42636
                     /locus_tag="PC471_000965"
     CDS             40555..42636
                     /locus_tag="PC471_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis cyclic di-GMP-binding
                     regulatory protein BcsB"
                     /protein_id="HDF2788104.1"
                     /translation="MKKFIISIISLVLFFSNISLIYKVNADETKVKNYKFERDITIDG
                     VIGSNSTFFEVNKNWDIEEVLLHLNFSKSQILNGDVSSLTVLINNVPIKSIKLNAKTN
                     YKNTLEVLVPKDYIIQGYNEIKIKTYKTISDKICQDDSNTGNWMVIHKESYISIRYKQ
                     KKVENSINEYPYPYAEIENNHKLDTTIVVPDNMTRGETTAVFNLASAFGKITKNDDLK
                     LDVKLYSEMKNWSDDNIIYIGKPENTAEEILDILSIKEQTLLSSNCIIKQVDSPYNKN
                     KKMMVVIGSNEDDLIKASNLLIENRLSNQVLSSSVLVNKETNIKINREQKLNLGHLTL
                     KDLGYSDFLLEGAFNQQALFDVKIPTGKVLDDGSKIILNLRYSDNLDFEKSLVTVSIN
                     DVIVGSKKLDRSHSNNDKLELKIPKDIDNKNYYQVKLTFNLSIKNSNCVTRESNNPWA
                     YVSNNSYLALSTKENETLSFENYPYPFVRDDEFNDLTVIMPDYSGSQAMTWMFRLGVT
                     LGANINSHNGNINVIRGKEFSDKYKDTNIVVFGVPHNNSVIKMLNNNLNIKFDKNYSN
                     FISNDKISFIDDYGKNISTIQLIKSPYNNQKNIMVISSMNEKNLYLGMDYLLNKSKVN
                     DLKGDTLIIDEYGEVEDLAYNLKSKKEVKDSSWNMSINKTTKVFLMISFITIIIVMIL
                     SMLYIKKYKRR"
     gene            42673..43758
                     /locus_tag="PC471_000966"
     CDS             42673..43758
                     /locus_tag="PC471_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HDF2788105.1"
                     /translation="MRKYMNEFKLKRSILISLIILIFIFIVYFVSIELKDRSLVNNQY
                     KGKIKSANLSVDYEINQVMKDIDKLGLNTVNVPIIINVESINSDIMSIDNNSKEKAIK
                     LIKKLNKKGISTILEAYPWIENGKLYETDWNPINKKRFFYTWQNAILNELIIDVANPL
                     DVNVLNIGSNFVHLEEYQQNWGEIIDFVQSKFDGLVTYRTNWWYTKKDDLSSKLFYEQ
                     KLNNNFFDKLDFISIAAYFELSNKPVNTVDELISALHSSTVNNRGQNIKQEIYNLYKA
                     HRKPIFFGELGFSNRESASSQPWNHTPSKAVNGEEQARCFEAYKKVFENEDWINGFSV
                     FCVGKIDDEKNFYPSKESIKVIKSWYE"
     gene            43812..44936
                     /gene="wecB"
                     /locus_tag="PC471_000967"
     CDS             43812..44936
                     /gene="wecB"
                     /locus_tag="PC471_000967"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HDF2788106.1"
                     /translation="MNDIKVMTVFGTRPEAIKVAPLIKELEKRENIKSIVCVTAQHRE
                     MLDQVIETFNINVDYDLDIMEKGQSLNDITCKILNKLPLILNKENPNIILVHGDTTTT
                     LATSLTAFYNKTLVGHIEAGLRTYDKYSPFPEELNRQLTGIIADMHFAPTNLARKNLI
                     SEGKPNNNIFVTGNTAIDALKMTIKENYNHPIIDEIGNDRMILLTSHRRENLGKPMKN
                     IFRAIKRIVDDFEDVQIVYPIHLNPKIRTIADEIFGKFPEKIHIIEPLDVADFHNFLN
                     KSYMIMTDSGGIQEEAPSLGKPVLVLRDKTERTEGIEAKTLKLVGTNEDRIYNSVSDL
                     LINKDNYVQMSKASNPYGDGNASKYIVDIIIKKFNCKYLN"
     gene            45014..45988
                     /locus_tag="PC471_000968"
     CDS             45014..45988
                     /locus_tag="PC471_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HDF2788107.1"
                     /translation="MARKLIKNLGKSKSVKRVKLLFKKIFITVFIVASIVAIFNITKY
                     FEELYKVRDLKSTKIEYYMDVADEAGDGKVQLSWKALLAIDMVIHDEDLSNIKKKDTL
                     DIGEKFIVEDKNGKGEKVYKVKKFNKVLSELKFDSSQKSRARKYMKDLEYTYLGNKQL
                     DSSDEKIKFIKKLEDSAIREYIDYGILPSITIGQAILESGWGNSKLTKQSNNLFGIKA
                     DKAWKGKSVEISTSEHYNEKIVASFRSYNSLQDSVKDHSLFLINNKRYRKHGLFEAKD
                     YISQAQALENAGYSTAEDKKGNRIYAELLIDVIRSYNLQLIDNKVETK"
BASE COUNT        18397 a         4678 c         7998 g        15058 t
ORIGIN      
        1 aataaagatt ttacaaataa atcttaagct atcttattta attctaatac aggaagaagg
       61 taaatgtata ttgaaaaaat catagaaata gaagtaaaca atttaaataa atacaaaggt
      121 ttgcaaaaga agtaatataa ttatatacaa tgaggatgtg agtatgatga acagactaaa
      181 gccaagaatt ttaggtatta gtatcttgtt tattattata attattttaa catgtggagc
      241 tgttttcaag tttaaattta aattagataa taaattaaaa gaaaccacac atacatattt
      301 gtctgagttt acagaacaca atataattaa tctcaagaca agacttagtg gtcaatttga
      361 catgcttgaa tctattgcta acttcatagg aaacttgaat aatataaatg atgaaatggt
      421 gattgattta atggattctg atgtaaagcg aagttcttta attagaatga ctatctctac
      481 attagatggg gaatgttatt ctaatgatgg aattacccat aatgttcaag atagagatta
      541 ttttaaaaaa gctaaaaatg gagaaaagaa tatatcagag cctttaaaat ctattgttaa
      601 taataaagaa ataatagttt tatctacccc agtttataaa agtgggaaaa tagttggtgt
      661 tttaagtgga atttatgatt caaaaaagtt aaatgagtta ttaggattat ctgcatttga
      721 tgaaaaagca gatgtttttg ttgcaaagaa aaatggagat atagttgctc aaaaaacaaa
      781 taaaaataga aagacgctat catgtatatt tcagcttttt gacaaagata attgtgatga
      841 agatgattgt ctaaaaacta taaaagaaaa tatgaaaaat gataagtctg gatattttaa
      901 ttataactta gataatgaaa aatttatgtt aaactatcag ccacttggag ttaatgattg
      961 gtatgttttt tctgttgtgc caaataaaat agtatctatt cagtcagaga atatatctta
     1021 tgatgtatat atttttgcat tagaaatact actagttata ttgattttta taatatatat
     1081 agcatgtatt ataaataata acaccaaaat tattataaat gaaaaagaaa aatttaaagt
     1141 actcactgat aatatattag gaggggttaa aacctgttta aaagatgaat atatgactat
     1201 gacatatgta agtcaggggt ttttagagct tacaggttac tcttgtgaag atttaaataa
     1261 aatatttaaa aatagattta aagacatgat ttataaagat gatttagaca agtttaattg
     1321 tattattcta aatcaattag aaaaacaaga taaaattgaa gttgaatata gaattgtaag
     1381 aaaagatgga agtataattt ggattttgga gagaagtaag tctataaatg ataataaagg
     1441 aagtgagtgt attcagggag ttttaactga tataacagaa cttaagaaaa tacaagaaga
     1501 attagattca aaaagatttg aaatacaggc tatcaatgat agtattgttg gtggaatagt
     1561 gataactgag ataaatgatg attttgatag tatatattta aatgaaggtt atttaaatat
     1621 gattggatat acacgcaaac aactggaaga tgagctaaat aataaattaa tcaaacttat
     1681 ttttaaagat gatagaacag aattaattaa tagtataaac aaacaactca aattaagtga
     1741 tacatttgca tctgaacaaa gagttgaaaa aagggatggc actattatat ggattttatt
     1801 aaaaggaaga aagatgattt atgataatgg aaaatctgta ctttgttgtg ttattgtaga
     1861 tattacttct gctaaattat tagagcaaga acttaaaaag agtgaagaac gtcatgaaat
     1921 cattctaaag cagacgcaaa atattataat agaatgggat atagaaaaag ataaaatgaa
     1981 tatatcacct aattggtata ataagtttaa atatgaccct ataactgaaa acgcaacaaa
     2041 aaatataatt aaatcagtag atatatatca tgatgatatg gataagttta tttcattaat
     2101 taaacttact tcacaaggta gtgagtatga agagtgtgaa attagactta gaactagaga
     2161 tactatttac gcttggttta gtataaaggc aacaacttta tttgataata atggtaaacc
     2221 ttataaagta gtaggaagtt tgactgatat tgatgaatca aaacgagaag ctgaaaagtt
     2281 gaaagaaagg gcagaaaaag accctcttac agatctgtat aataaaaaag catcacaatc
     2341 acttatagag gagtacataa cattggataa aaaaagaaaa ggtgctttaa tgattattga
     2401 tattgatgac tttaaaggaa taaatgataa tttaggacat ttatatgggg atgctgtatt
     2461 aagtgaaatt gcaaatgacc taataaatct atttagagca accgatatag taggcagaat
     2521 tggtggagat gagtttattg tatttataaa agatatttct gaaacaagag atattataaa
     2581 taaagcagaa gaattggtaa aaacatttga aaggtctttt ttaggtaaag gacgaaatta
     2641 taaaatatca ttaagtgtag gaattgctaa gtttccacaa gatggtatgg actatatgac
     2701 actatttaga aatgcggata tagctcttta ctcagccaaa gggaagggca agaattgtta
     2761 tacattgtat gataaaaaat ttgataaagt ccaatatata actaatacaa gaaatgcagg
     2821 agatagtgat aatgtagaga tgtcttttaa agaacatatc acaaattata tatttgatat
     2881 actatatgaa tcaagtgata tagaagtggc agtaaattta attctaagta ttgtaggtaa
     2941 acactttaat gtgagtcgca tgtatatatt tgaaaattca gatgataatc gagtatgtaa
     3001 aaatacattt gaatggtgta acgaaggtgt aacctcggaa attaataatc ttaaaaatat
     3061 ttgttacaag gatttaggca actatctttt aaattttaat gcagatgggt tattttattg
     3121 taatgatatt gaaaatttag gtagtgatgt gaaaaaagta cttaagccac aaggcatacg
     3181 ttcaatatta caatgtaaaa ttttagaggg taatgaattt agagggttca taggatttga
     3241 tgaatgttca tataatcgta tgtggactaa tgatgaagtg aacactttga catttatatc
     3301 taaaacgctc agtatgtttt tattaaaaat gcgtattcag agtaagctag aagagtcttt
     3361 aacaattcaa aaatctgtga tggataatat ggatatatgg gcatatgttg ttgacaggaa
     3421 tacctatgaa ttactattta tcaataaaaa aatgcttgag tcagcaccaa acactcatgt
     3481 aggtgattta tgttataaag cgatatggaa taatcaagat aaaccttgta aaaaatgccc
     3541 tatgatatat ttaaaagatg gtgatgatac ttataaatca ttcatgcata ataaaaagtt
     3601 tgaggtacag gcaaatataa ctacaactac tttgaaatgg tttgatggta aagatgcatg
     3661 tttaatattt ggaagtgaaa taactagtat tgaagctgaa gaaaatactt aaaatactgt
     3721 gttatatata tacttaaaat cgcaatttga atttattaga cttaaaatgt ctatataaat
     3781 catttaagtt gtggttttaa gtatttttta tgatataatt aagcgttaag aacaaaatta
     3841 aaagatactt ttgtataaaa aaatatttgt taaatatata ttgagcaggt gataatatgg
     3901 aaaataagtt tttaccaata agcaaacaag atatgataga tagaggatgg gaagaattag
     3961 attttgttct agttacagga gatgcttatg tggaccatca tagttttgga acagcaataa
     4021 tatctagagt tttagaaagt gctgggtata aagttggaat aatagctcaa ccagactgga
     4081 gaactacaga tgattttatg aagctaggaa aacctagact tgcatttcta gttaatgcag
     4141 gaaatatgga ttcaatggtc aatcattatt ctgtaagtaa aaagcataga gaaaaggata
     4201 tgtattctcc aggtgggaaa atgggttata gaccagatag agctactata gtatattgca
     4261 ataaaataag agaagcatac agtgatgttg caatagtaat aggtggaatt gaagcgagct
     4321 taagaagatt tgcacactat gattattgga gtgataaggt tagaaagtct atgttaatag
     4381 atagtggagc agatttatta gtttatggta tgagtgaaaa gcagattgta gaagtagcca
     4441 atgcacttaa tgatggatat gacccaaaat acattagaca tattgatggg acatgctata
     4501 tttcagatac attggaagaa atatatgata aatatatatt aattccatca tataaagaaa
     4561 tttgtgaaga taaaatgaag tatgtagaag cttttaaaat tcaatatgat gagcaagacc
     4621 catttagagg taatataatt gttcaacctc atggaagtaa gtatctggtt caaaataagc
     4681 cagaaaaacc acttagtaga gaagaattag atgaagtata tggattacca tatcagaaga
     4741 cataccatcc tgtatatgaa aaatttggag gaataccagc tatagaagaa gtaaaattta
     4801 gtatagtaag ttcaagaggc tgttttggta gttgttcatt ttgtgcaata acatttcatc
     4861 aaggtagagc agtgcaaagt agaagtgaaa aatcaattat tgatgaagca ataggaataa
     4921 caaatttaga tgattttaaa gggtatatac atgatgttgg aggtccaaca gctaatttta
     4981 gaagaccagc atgtaaaaaa caaattacta aaggtgcttg taaaaataga caatgcttat
     5041 ctccatcacc atgtaaaaat ttagatgcag accacagtga atacttacat ttacttagag
     5101 ctgtaagaaa attacctaaa ataaaaaagg tgtttgtgcg ttctggaatt agatacgatt
     5161 atgtaatggc agataagaac aataaatttc ttagagagtt gatagagcat catgtaagtg
     5221 gtcagttaaa ggtagcccca gaacatatat cagaagaagt tttaaagtat atgcaaaaac
     5281 cagcaggaaa aacttatgat aaatttagac agaagttttt tgctataaat gaagaattag
     5341 gtaaaaagca atatttgata ccctatttaa tgtctagtca ccctggttct accttaaata
     5401 gcgctataga gttagcagag tatttaagag atactcacta ccaaccagag caggttcagg
     5461 atttttatcc aactccaggg actctatcta caaccatgtt ttatacaggt attgacccgc
     5521 ttacaatgaa gccagtttat gttcctaaat caaaacgtga caaggcaatg caaagggctt
     5581 tattacaata tagagcacct agaaattatg atttggtata ctctgcgtta gttgaagcag
     5641 gaagagaaga cttaattggt tttggacata gatgtcttat aaagccaaaa aatgaaaagc
     5701 cttattttaa tagaaataat tctaaaaaaa atgtatctaa aggaacaaat aaaaacaaaa
     5761 aaactaatac aaataacaga agtaataaaa atcaacaaaa aagttcaact aaaaaaaaga
     5821 aaaaacatta aagtataaat aaaaatactt aaaaaagaag gattattcga taaattaaca
     5881 gaaattagta taaataaata taattatttt aatgacgtta cctttgggag gaagagatga
     5941 aaaagtatat ttctataata ttattagtgg tgttgactat ggttgttttg gtaggatgtt
     6001 caccaggaaa agataaccct aaaaataagg agctatcagt tgtcaaacaa gtaaaagtag
     6061 ctgttccaga tggactacca gcagtggcaa tagctaaact tgcaaatgaa aatccagaga
     6121 ttaaagaagg atatgaaaca gtgtatagca ttgaaaagac acctgaagcg atttccacta
     6181 gagtgatgaa aaaagatgca gatatagcta tagttccttc aaacatggct gcaatagcgt
     6241 ataataaaac ttcatcatat aatattgttg gaactgtagg aatgggttca ctttatttgg
     6301 tgtcaacaga aaatataaaa gattactctg atttagttgg taaagaagtt ggatgtactg
     6361 gaaaaggact tactccagac ataactataa aaagtctttt gttacaaaag gatataaatt
     6421 attcagatat aaaatttaat tatgtaaatt cagcaagtga actggtacca ttattggcta
     6481 ctggtaaagt taaaacaggt attgttccag aacctgcact tagtgctttg atgtctaaaa
     6541 atccagacat aaaaattata aaaagtttaa atgactcatg gaaggaagta agtggttcaa
     6601 aagatggtta tcctcaatct actttaattg taaagtctga atttttaaga gataataaag
     6661 attttgttga ttcttttgta ggtcaattat caaatagtat agattgggca aacaaaaatc
     6721 ctgaagaatt aggtgcatat tctgaaaaaa taaaaatatc tactgaatca aagataatag
     6781 gaaaatctct tgaaagagct aatttgaaat atattccagt taaggacatg attaaagatt
     6841 ataaaaatta ctatgagaaa ttagctaatt ttgatgataa gacattagga ggcaaagtac
     6901 cagatgaagc aatatacttt gttgagaaat aaaaaaaata aagaacaatt tatagataaa
     6961 atggaggtaa cactttcttg tgttatcctc ttatttttat ggcaaattat tgctttaaaa
     7021 attgataatg atattttttt accaacagta ggtcaagtgt ttaatagtat aaaagagatt
     7081 atgctaaatt ctagtttcta tatagatata ctttactcta tggggagatg tttttttagt
     7141 tttttactgg ctatgatttt tgctattata ctttcaatag tgtcttattt aaatagattt
     7201 tttagaaact tattaaagcc aatcaatgct ttgacacgtt caataccaac tatgatattg
     7261 gtagtacttg cacttatttg gtttgaaaag gatagtaccc cttttatagt tggatttact
     7321 atagtttttc ctatattgta tgataacgtt ttgggcgcta tattgaatat agacaagaac
     7381 ttattagaaa tggctaatgt gtataaaata aggtttatag ataaggtgtt aaaaatttat
     7441 ttaccagcca taaaatttca aattatatct atactagtat caacattttc tttaagtttg
     7501 aaagttgcta tagcaggaga ggtgtatagt caacctacat atggtattgg aactatgata
     7561 cagactgaaa aaattaactt taacacatct ggaatatttg catggataat tattgttctt
     7621 ttaatatctg ctattttaca aatagctcaa aagtttttag caaggagagc ttttttatgg
     7681 aaaagataaa gttgagtatt gaaaatataa ataaaagata tgatagtcgt attatattta
     7741 gagattttaa tattgatttt tatgtaaatg aggtaaattg tatccttgga aaatctggtt
     7801 gtggaaaaac tacactcttg aacataataa gtgggattat taaaaatgac accaacaatc
     7861 taaatatcaa agaaaattta aatagagtag gaaataagct tgaagctagt tacatatttc
     7921 aagatgatag acttatagac tggttaacag tagaagaaaa tataaagata gttgtaaata
     7981 aatactataa taaaactcaa ttaaataaaa tttgtgatga atacttggaa ttggttggaa
     8041 ttagtgatta taaaaaattc tatcctcaaa tgcttagtgg aggtattaga caaagagtaa
     8101 atattgctag agcttttata tatccatcaa aaaacattat aatggatgaa ccttttaaat
     8161 ctattgatgc aaagaataca cagcttataa tggataattt tagaaatata ttaagaaaag
     8221 aaaagagaac tgtgttattt gtaactcata atattgagga agcattgttt ttagcagata
     8281 ggatatttat tttaggagac tctccaatta gaataaaaaa aatattaaaa aattctaagg
     8341 aattggaaaa aaatgaagtt ttgaagttga tatagagtta taataatact gtcgttttaa
     8401 aatatatttg gttaaaatct ataagactat attaaaatag tacagaatat tcaaaactgt
     8461 gtataatgtt cgtattttat aaaaaggaag tgaatgtatg gttaagacta atgctatgag
     8521 aatattagat tcaaataaga ttgattataa agtaatgtct tatgaagtga aaagtgaaca
     8581 tgtggatggt gttgaggtgg cacatgatat aggaagagat gtaaatgaag tgtataagac
     8641 attagtaact caaggagtta gtaagaatat atatgtatat gtaataccag tacatgaaaa
     8701 cttagactta aaaaaggctg caaaagtagc taaagagaaa agtgtagaga tgattcatgt
     8761 aaaagatata aataagctta caggttatat tagaggcgga tgttctccta taggtatgaa
     8821 aaagttttat aaaacttttg taaatgaaag tgcaaagaat ttagatacaa ttattgtgag
     8881 tgcaggaaaa ataggatatc aaatagaact atctccattt gatttacaaa gacttataaa
     8941 agtagaattt gtagatgtaa taaaaaaata agaattacat ataaaatata gagcttatat
     9001 tctcaataat aggtgttgta atattaataa aatgaataag aattttaaaa taaatattca
     9061 gttacttatg tgtaattggt ataaaaatat tgagaaattt taaaaacaat ggtaaagtgt
     9121 aagtataatc acgaatattt tttataaagt aatggaggga tgttcgcttg agaaaaataa
     9181 aatctgaaca aatcgtcgag caggtaaaaa aactttgtat agaagcgagc ctatatttag
     9241 gagaagatgt tttaagttgt ataaaagaaa aagcgaaaag cgaaaagagt gaagttggaa
     9301 aaaatatatt gaatatatta gttgaaaatg ctgaaatagc taaagaaaaa aatataccaa
     9361 tatgccaaga tactggtatg gctgtatttt ttgttgaaat aggacaagaa gttttaattg
     9421 aaggagacac acttactgat gctataaatg agggagtaag acaagggtat gaagaaggct
     9481 acttaagaaa atctgttgta agtccaatta acagagtaaa tacaaaagat aatacacctg
     9541 ctgttattca ttatgatatg gtaaaaggtg ataaaattaa aatagaattt gcagctaagg
     9601 gatttggaag cgaaaatatg agtaagatga aaatgttaaa gccatcagat ggattggaag
     9661 gaattaaaaa attcataata gatacagttt cagaagctgg acctaaccca tgccctccta
     9721 tggtaattgg agttggtata ggtgggactg tggacaaatg tgctcaaata gctaaaaagg
     9781 cactttttag agaactaggt gaatttaata aggacgaaaa tatagcaaaa cttgaaagtg
     9841 aactattaac agctattaat aaacttggta taggacctca aggcttaggt ggtactacaa
     9901 cagctttggg attaaacata gagacttttc ctacacacat agcaggtcta cctgttgttg
     9961 taaatataaa ctgccatgca tcaagacata agaatgtagt aatttaggag gaaaataaaa
    10021 tgataaaaat aactacacct gtaaatgaaa ttgatatagc aaaattaaat tgtggagata
    10081 caattagttt gagtggtata ctttatacag caagagatgc tgctcataaa agattgatag
    10141 attgtataaa taaaggtgaa gaattaccat ttgacgtata tggtcaagga atatattatg
    10201 taggacctac tcctacaaag ccaggagaag ttataggtgc tgcaggtcca acgacgagtt
    10261 atagaatgga tgatttgact atacctcttt tagaaagagg tcttagattg atgataggca
    10321 agggaaaaag aagtgatgaa gttatagaag gtatgcaaaa atacggagca gtttatcttg
    10381 ctgcaatagg aggagctgga gcgtatattt caaattcaat aaaatcatgt gaaattattg
    10441 catatgaaga tttaggagct gaagctataa gaaagattgt agtagaagac cttaagctta
    10501 cagtagcaat agatagctat ggaaataata tatatgagca aggtcgagca atttatgaat
    10561 gtaaataatg tttagaaata aatatgaata tcaaaattaa aaatagttgt gtatatttta
    10621 aggaggaaaa ttgatgtcta aggattatgc aaaattggca ttagaaatgc atgaaatcaa
    10681 caaagggaaa gtaagtgtag aaagcaaggt agaaattaaa actaaggatg atttatcaac
    10741 agcatataca ccaggggttg ctgaaccttg tttaaaaata catgaaaatc aagatgatgt
    10801 ttatagatac acttctaaag ggaatctagt agcagtagtt agtgatggaa gtgctgtact
    10861 tggattaggt aacattggtg ctgaagcatc tatacctgtt atggaaggta aagctatatt
    10921 atttaaacaa tttgcagatg tagacgcttt tccaatatgt ttaaagacta atgatgtaga
    10981 tgagatagtt aaatctgtag agttgatgga gcctgtattt ggaggtataa atttggaaga
    11041 catatcttct ccaagatgtt ttgagataga agagagatta aaaaaatctt taagtatacc
    11101 tgtttttcat gatgaccaac atgggacagc aataatagtt gcagccgcca taataaactc
    11161 aataaagtta attgaaaata aaaaaataga agatttagaa atagttataa acggagcagg
    11221 agctgctggt atagccatag caaagatact acttaatatg aatgttaaaa atataatctt
    11281 atgtgataga agtggagcat tggaagctag tatagaaaat ctaaactatg ttcaaaaaga
    11341 aatgttaaaa gtaacaaata taagaaatga aaaaggacct ttaaaagata ttataaaagg
    11401 aaaagatgta tttattggtg tttcaggtcc aggagctgtt acaaaggaaa tggttgaaag
    11461 tatgtctgag aagccaataa tattggcaat ggcaaatcct actccagaga taatgccaga
    11521 agaagcaaaa ttaggtggag catttataat gggaacaggt cgttcagact tccctaatca
    11581 agtcaataat gtactggctt ttcctggaat atttagaggt gcacttgatg ttaaggctaa
    11641 agagataaat gaagaaatga agatagctgc tgcatatgca attgctaata caataagtga
    11701 tgaagaaata tgcccagagt atatattacc agatgtattt aataagaatg tagtaaaaaa
    11761 tgttgctaag gcagttaaag aagcagcgat aaaaactaaa gtaaatagaa tataaatttt
    11821 tatatgttca tatatcctta tattatcggg tatatgggca tttttattat aggtttatac
    11881 tcatatatcc ttacttttat atacatatct ttatgattaa aggtgttaga aaatgtagta
    11941 aattttatac tatcagactc tatctttatt actttagtag atacatggtt gtaaaggttt
    12001 atagaaaaaa atataggaag gcagattttt attgttgcgt atatatgaga atgatataat
    12061 atgaatatta taaattaata aatttaaatt tatggaggta caagagatga tggaatataa
    12121 agttaatgac caagaactta atgcttcaat gtttatccca ttcgtcaaca ggatatggcc
    12181 aggagattat gatatggata aaacacagac tgcattatca aaaactttga atataactgc
    12241 ttatgatgat aaagtgcttg taggatgtct gcgagttctt tcagatggtt attattttgg
    12301 aacaattaca gaattgcttg ttcttccaga gtatcagaaa caaggcattg gtagtaagct
    12361 tttccagctt gctaaagaga acaccccaac tatgctatat tttggtgcac agccaggagt
    12421 agaagcgttc tatgaaaaaa atgggtgcca aagaagtctg caatcctaca taataaaaaa
    12481 gaaagatagt tattaaagtt ccagctttca ataatcttaa aagtacaata ttttagtgga
    12541 atcatttcat tttttattac taatatgaca taaacatgtc atattagtat tgatatactt
    12601 ttaatatggt taagagccaa agaaaaatat aaggagtgat ttaaaatgga agaaactaaa
    12661 ttttgccaat gctgtgcaat gccaatggga caaactgatg aattatatgg gacaaataaa
    12721 gatggaagta aaagtactga ttattgtagt tattgtttta aagatggtga gtttacagct
    12781 gatataagta tggaagagat gatagaagtt tgtatccctc atatgcttca aagtaataag
    12841 gatatgactg aagatgaagc aagaacaatg atgaacaatt tctttccaac attaaaacgc
    12901 tggaaataat tttatgcaaa attagttata tgcaaaacat gaaatatggt gttgagaata
    12961 aggggtggtg tgctttatag cataccacct gttttgttac ttggagttca gttattttcc
    13021 taaatatttt aaatatacta ttttagataa gttccctctt catcttgata aattaatcct
    13081 tttttcataa gcactccaag agctcgttta aaatttttct tacttgaatt aaataatata
    13141 gaaatatctt tagggtcaga tttatcatta aatctcatat atccatcaga accttctaaa
    13201 taacttaaaa tcttattttc taaagtatca agttcatcct ttctatttcc tctaggagaa
    13261 agaccaagtt ttccatcttc atatattttt atgacatgta aattttcaag aacatcacca
    13321 gtttttagtt cagtaaagta ctcattatga agtattacac cagcatattt attgtctaca
    13381 catatcattg cagaattatt agtttggaat ccatatacaa ctccagttac aatatctcct
    13441 acattgtaag tatggtctgt taataagtat gaatctatgt ctgtagtagc agcaattctt
    13501 ttgcttttgt ctaaatatat ataaaataaa tatttttcgt ctctaaatag tggataagtc
    13561 ttagctttaa aaggaactaa tatgtcttta ggaagtccca tatctataaa agtaccaatg
    13621 tctgtattat caactacttt taaatgtgct acatccccaa ccttagctaa aggcggtatt
    13681 aaagttgcag cagttctgtc atcagaatct ttaaatatga aagcatttac ttcatcacct
    13741 atgtttattt cattttttcc gattaatcta ttatgaagaa gtatatcgtc ttttgtgtta
    13801 ttagtttgtc catccaaaaa atatccaaat tcagtttttc tttttactgt aagtttatta
    13861 aaatcaccta tgtttatcaa attaaaatca tcctctcatt ttatatttat aatttagcca
    13921 taatatactt atgtagtagt ttgtcacagt atccatatta gtattagtga aatttacaat
    13981 aaataatata gttatttatg tatttttcaa ttctctagct tcttatatag tataaactcc
    14041 aatttctttt ttgtctttag gttttattaa aatttttaca aaataagtta aaaagtattg
    14101 aaataaaatt tcttttaaag tataattggt atataccaaa attggtatat accatatcga
    14161 aaagtggtga ctatatgaaa atagtaaata aatctagtaa tataccattg catactcaat
    14221 taagttcaat aataagagag atgatagaaa ctggtgagct taaagagggc gatgcaataa
    14281 tgccagaaag agaactttgc aacatacaaa atgtaagtcg tatgacagta aataaaacca
    14341 ttgttggatt agttacagaa ggacttcttt atagggtaca aggtaaaggg acattcgttg
    14401 caaagcaaaa aaagaagtat cagttttcaa atgtaaaagg ttttactgat gttatgaaag
    14461 aaaagggagt aaacataaaa acggatatat tatcctttga gatggaacta ccagatgatt
    14521 tagttaaaag aaagcttgga ataagtgata atactacaaa tatatataag attgtcagac
    14581 ttagatatac tgatggagag ccatttggat tagaaatagt atatctatca gaagaaatgt
    14641 gtaagggtct aacaaaaggg atattggata attcttcatt atatagggtt ttaaatgaaa
    14701 aatatggcta taagatacaa aaagctgaac aggtaatgga gccagtaata ctttcagatg
    14761 aagaaagtaa gcttcttgaa acagatgaag gtgctttagc tcttaaactt catagaaatt
    14821 cctataatag agaaggtagc cctatagaat ataccatatc aatatttaga acagataaat
    14881 atcaatatga aatagtattg agtgagtagg tgaataatat gaaatgttta attaatggaa
    14941 agataatatt aaaaaatcaa atcttagaaa ataaagtatt agtatttgat gaaaaaataa
    15001 ttgatatagc tgatagtgta ccaaaagatt gtgaagttat agatgctgat ggaaaatata
    15061 tatctccagg gcttattgat attcatatac atggaaacat gggaaaagat acaatggatt
    15121 cgactgatga atctattgaa acaatttcaa agtcaattat gagacatgga gtaacatctt
    15181 ttcttccaac tacaatgact atggataaag aacatgttta tgatgcactt gaagttatta
    15241 aaaaagccca aaatagaaag cttgaagggg cacaagtatt aggagcacat ttagaaggac
    15301 cttttataaa tgagaattat aagggagcac aaaatgaaaa atttataata aattcaaaat
    15361 acgaatttat aaaagaatat aaagatgtta taaaagttat aacttatgct ccagaaaaag
    15421 atattgattt tgattttact agagaaataa aaagatgtac agatatagtt ttatctattg
    15481 gtcatagtaa tgcaaattat gaccaagcta aagaagctat aaatttagga gtaactaatg
    15541 taactcacat gttcaatgca atgactggtt taaaccatag agacccaggc gttgttggtg
    15601 cagcacttac aactaatgtg tacagtgagt taatagcaga tacaatccat ataaataaag
    15661 atttatttca atttatacta aataataaag gaaaagaaag actgatttta ataacagatt
    15721 ctatagaagc tggtggtctt gaagatggca attattctct tggaggtcag aaagtcatag
    15781 ttaagggtaa tgaagctaga cttgaaaatg gagcattagc tggaagtgtg ttgtctttaa
    15841 ataaaatggt atttaacttc ttagataata caaaccttaa agtgaatgaa gcaattaatt
    15901 tagcttcttt aaatccagca acatctttgg gaataaatga caaaaaagga agcttagaaa
    15961 taggtaagga tgcagatata gctgtatttg atgagaattt agattgtaaa atgacacttt
    16021 gcttggggga cgtagtatat aaaaatatat agtatacaaa acaaacctta aagacagtaa
    16081 aaacaatata aaaaataata aattaaactt acattagtaa aagtagtagt taaaatttag
    16141 gaggaataag atgagagtat tagtgtgtaa agattatgat ggaatgagta aaaaagccgc
    16201 tgaaatgata gcagctcaaa tagttttaaa accaaattca atactgggac tagctacagg
    16261 aagtacacca gtaggtatgt atagagattt agtaaaaaaa tacaatgata atatagtaga
    16321 tttttcagat gtgatgagct ttaatttaga tgaatattat aagttaccaa tatctaatga
    16381 ccaaagctat gattatttta tgaaagaaaa tctatttaat catgtaaaca taaagccaga
    16441 aaatactcat ctaccaaatg gaatggctga tgatattgaa aaagagtgta tgaactatga
    16501 agcttctata gatgcagctg gtggtataga tgtacaagta cttggaatag gtcgtaatgc
    16561 tcatataggt tttaatgaac ctgatactaa atttgcaaag agaactcatg tagttgaact
    16621 tacagagtct acaatagaag ctaatgcaag attctttaaa tcaagagaag atgttcctaa
    16681 aaaagctgta agtatgggaa taggttcaat attgaagtct aagaaaatat tgcttttagc
    16741 ttcaggagaa gaaaaagctg atgcagtata taatactgtt tatggagata taactccaga
    16801 agttcctggt tctatacttc aacttcataa agacactata gttatagtag atgaagcagc
    16861 agcaagtaaa ttgaatccaa aagattataa attagtataa gaatagaatg tagtttaagt
    16921 aaaaaatagc tatcattgag taatcaaatt tatttaatga tagctatttt tatttaaatt
    16981 ttttacttct ttttatttaa attagttatt attgtttaaa ttacttgctt attacttttt
    17041 acgaaaactc gttatcaaga agtatccaaa taagatgtta aaaattatta tgtttagtgg
    17101 gattctatca agcataataa aattacctat aattgggata ttaccaactg gaaacataaa
    17161 gaagaataca ggcatcagtg atagtaaaaa agatggaaaa caaagaagaa gtaatcttgg
    17221 aatatcaata taccaactac catccttttt gtattctcta atcaataatt cagttcctag
    17281 taataaacca ataccaccag aggaaattaa attaataaaa atgtatcttt taaagtcttg
    17341 catataacca gtgtaaaatt gagtatagaa atcataattt aatttaacag ctaaaaaata
    17401 cataagcaat agaatgatta aaaataaaag atatctaaga gcttttttca ttttaaattc
    17461 tcccttcaga aatactttat tcagttttat tttgttggaa tctgatttta tcatttagtt
    17521 taagaaatcc ataatattag tgaagaatga aataagtatt atatatgaat aaatattttt
    17581 agaattaagg tgaattgtag tgcttaatat aactataata ttaattgttt gaatattcaa
    17641 taaaaagttt gaaaaatatc aattttgata taaactgaat aaatacagtt gaatattaaa
    17701 ttattatttt atcaaaaata tgcaaagtac tacaccttca aatatcacct aaagtaatta
    17761 aaaggcaatt aaggtgaagt agttcaagta tttctaaaat aagtttaata aattctataa
    17821 taatttattt tttgccttaa aattgcctta aaatccaaac aattttaaaa ctaatgtact
    17881 ataaaaagct ggtataattt aaatatagta aaaaataaga tttatcttat ctattgggat
    17941 ttaattattt aatcaatata tttataccat ttaatttaaa taaaaagtct attaaggagt
    18001 gagcttatga gtaagttaat ttatatagca gatgatgaag ataatataag aaatttggta
    18061 aagacattct taaaaaatga aggtcatgat gttatggatt ttaaaacggg tgatgaactt
    18121 ttagaacaat ttaatataaa ggagtgtgac ttagtaatac tagatataat gatgcctggc
    18181 tcaagtggtt ttgaagtatg tacaaaactt cgtgagaaaa gtacagtccc aataatcatg
    18241 cttactgcaa gagatacaga tatagattat attacaggga taaccttagg tagtgatgat
    18301 tattttacaa aaccatttag cccaatgtct ttggttatga gagtaaaatc tatatttaga
    18361 aggatagagt ttgaaaagaa acaaaattat gataaatata gtaactcgat agatatggaa
    18421 ttaaaatttg gtgatgtaat aataaataaa aagaataaaa tcgttacaag taaaaatgta
    18481 aatatagacc ttacaccaaa tgaatataac ctattgactt atttgtttga aaatatagat
    18541 agagcagttt ccagagatga attattaaac aaaatatggg gatacgatat agaagttgag
    18601 acaagggctg ctgatgatac agtgaagaga ttacgtaaaa aaattttaaa tacaaatatt
    18661 ttaatagaaa ctgtatgggg gtttggattt aggttgaagg agaaaagtta agatgaagaa
    18721 tggaaatgac aagaatagaa ttggaaagat taaatccaaa tttaaattaa agttctcaaa
    18781 gaaaaatata agcataaaaa ctaggatttt gttgagtata ctttgtttga taattatagt
    18841 atttatgata atattagtat cttttaatgt atttcttgat acatatataa aaacaactgc
    18901 gaatgaagaa ttgacaaagt ctacagaaat agtagaacat atggatagta attttagacc
    18961 tattaagcca ccacaagatg atggaaagct tcctcccaaa gtattatcta attttatgag
    19021 aaatgtacaa gataaagtta ggatggcaga aacacaatca gatgctgatg ctatggtagt
    19081 tgattctaag tataacctga tatttccaac aagagaggat gatttcttaa aaaatgttga
    19141 tgagatggag ctgattaggg agtgtattca aaatgaaaaa ttagatttaa attcagatga
    19201 aaatacacgg atatctacca gaaataagga ttattatatt tcaactgtaa aaattactag
    19261 tatatatggt gaacaagaca agtatcttat actttttata gacatatcaa aaactctaaa
    19321 tctagcacaa aagataaata ttgtattaat atctgtaatg tgttttgctg gaatattagc
    19381 aatatttaca gctgtgattt tatctgagaa aattgctaaa ccgataaaag agttgtgtga
    19441 gtttgcaaaa aaaatgggtc aaggagattt caaaagaacc tactttgact tttcagataa
    19501 agaattagta gagctctcaa ctgttatgaa taagagtgct gagtatctgg ataagtatga
    19561 taatgaacaa aaaatatttt ttcaaaatgc atctcatgaa ttaaggacac cacttatgtc
    19621 tattaaaggt tatgcagaag ctataaaata taatgttata gatagtaaac atgcaagtga
    19681 tataattctt gaagaaagtg ataggttaag tgatatggtt gaagatttat tatacatttc
    19741 taaaatagat aatataacta aagattttga acttgtagaa tgtgatttaa gagaagtact
    19801 ttcaaattgt ggagctaggc aaaatgtaag agctataaat aaggggataa aatttattta
    19861 tgaatttgat gaagaaatgg ttttatttga atgtgatgaa aaaaacattt ccaaagcatt
    19921 tatgaatctt atagaaaatg ctttgagata cgctaagagt gaaataaaaa tagtgtgtaa
    19981 gtataatcaa aaaaatatag ttgtgataat agaagatgat ggaattggaa tcaagaaaga
    20041 agatttacct catgtttttg aaagattcta taagggagta gggggaaatc atggaattgg
    20101 attatcaata gttaaatcta ttgtaaataa gcatggtggg cgtatatacg tagaaaatgg
    20161 caaaaaagga gctaaattta ctatattatt taaattgtaa aaaaattaga gaaaatatag
    20221 agatgtttga agttgttttt tatatatatc tgttgtatac tgttgaaaaa agaacaatat
    20281 taagctgaca tatattacga tagttgacat ttaaagtaaa tgcttaaata tagatacgaa
    20341 aggaaaagta aaatctgaag tgtatttagg tttaagtaag cttcttattt tataaaaaat
    20401 atgtatgaaa gaacagctga tttttaatat tacaaattta gtaacggttg tatttgaagc
    20461 ttttgtcatc catatgtttt tatcagactt tttaggtcag aaagaagggt atataaaagt
    20521 tgtaagatat gctaaactgg gttttataat atgtttagga ttctgtaatt taataacttt
    20581 aaaccctaaa atcacaatgc ctttaatttt catccttatt ttttcaacat catttttata
    20641 taaaggaaat ttaaagacac gattgtttac tactgtttta ttaagtatat tctttatact
    20701 gtcagaaatt gtagttacaa gtatttttgt tttatttgta aaagaaggct ttgaaataat
    20761 gcttgaaaat aatagtataa gagtattggc gacaatttta tctaaaattg tctttttgtt
    20821 gacttgtaaa attatttgtc tatttaaaaa ggatgtacat ttagatatgc ccataaagta
    20881 ttggctccct ttatttttaa tacctatatt tagtttattt ctgtctgttt ctatctttga
    20941 tgtaagtaaa ttttttagtc ttgaaagttt aaaatttcta agcttaatat ctagtgttgg
    21001 tattttatat attaatttta ttgtctttta tttatttaaa tttattatag ataaaactaa
    21061 actatctatg aaatatgaac tacttgaaaa agaaataatt cataaggaag aattaagact
    21121 tagtaatgag tgttataaaa ttattgttga acagactgat tctgtcgttt tcgaatggaa
    21181 tatcaaagaa aataaatcat ttgtatcgca ggcttggact gaaaaatttg gatataacaa
    21241 tgcatgtaag aatattttta aagaaattaa agataaagac ttagttcatt ctgaagataa
    21301 agctatattt gaaggttttc ttgaaagtat taaaaaaaag aatatgcaca atcaagcagt
    21361 atatcgttta aaaaaatcta atggtgaata tatttggtgt agaacatcaa ttacaagcat
    21421 ttataatgat gaaaatgaac ttttaagagt tgtcggagta attgtagatg tagatagtga
    21481 tataaaaaaa tatgaagagt taagaactag agcagaaagt gactcattga ccaatatata
    21541 caataaaggt acatttgaaa aattagtgga agaaactata gttatgaata caggtgataa
    21601 aaaagatgca ttattcatta ttgatttgga tgattttaaa gaaataaatg ataattttgg
    21661 tcatccattt ggtgattttg tactaaaaac atttgctgat aagatacaaa ctagttttgg
    21721 tagtaaagac ttagtcggaa gaattggtgg agatgaattt gttgtatata tgcaagatta
    21781 tgttacagaa gtaaatttgc ataaaaaagc taaagaatta aatagagtac tttcggataa
    21841 ttacactgat ttgagtttta gttttgatgc ttctgtcagt ataggtattg caaggtatcc
    21901 tcaagatgga acttcatttt ttgaattgtt taaaaatgca gatagagcac tttatagcat
    21961 aaaagcatca ggtaaaaatt catattgttt atttgaagaa gaactatatg tacagtgaat
    22021 ttgaatttag attttatgtg agagtaagat ttatatatac ttgtggctgt ctgatttata
    22081 aaatatgtta ctttgtatat ttttatatgt ttcagtaagg ttaattaaca tgagcaaagg
    22141 tgtaagtaaa tttatattag tttaagttta cttacacctt tataaaaata caaataatag
    22201 attcatttac attcttaaat gattaaagct ctttaccttc taaacatgca taacatatcg
    22261 ttataattcc agtaaataag actaaaatgc ccattactaa gattacaatt tcatcatcta
    22321 ttactccaaa tgtttttaaa gtaacttatt tcgctttgta tcataataat ttgtctcttt
    22381 ttacatttta acttctgctg agatttgagt tagatattca ttagaatctc gcacacttat
    22441 atcatataga agatattctt cataaacaaa atccccaata cgtaaattat tctgattaaa
    22501 aaaatttaat aacttttcat aagccttgta aatagtttca taactccctt tatgataagc
    22561 agttacataa agtccctttg gtttgattga tacaggatat ttcttgtact ctttattaac
    22621 ttttgtaaat aaatatgaat atgattcaaa gtctttatta attatatctt cacctttaat
    22681 gattgagcca atcgtaccat aatcactcaa ttcatagttg tccataaagt taatacattc
    22741 atataaaaaa ttttcttcct catctcctaa aaactgttct ttatatgtaa caggtgtttt
    22801 gaccaagtac tcttcttcat gttcttttaa agtaatagta ttcgcatcta tattacatgc
    22861 acttttagta aatgaaatag tagattccat tagagcttga atattattta gtttttcaat
    22921 ttcattttta atatcaatgg ttttcttttc taaaagctca attagtaaat taggtgttct
    22981 tttgtctata taagctttaa tttcttttaa aggcattttt acttccctaa gtgaagaaat
    23041 tataccaaaa atgctaagtt gatggtatga gtagtatcta tatccatttt ctttctttaa
    23101 ttctggtgag aaaagaccta tatcatcata atggaaaagt gtttttttat tgattccaca
    23161 aatctttgca aattctccag ttgtaaaata attttttgaa acttgactca taattataat
    23221 tcctccttga ctatcctgtt acagtatagt ttatcatagt attttgtaaa ggagtgtgat
    23281 tatatgaaaa aattaattca ttttttaaaa ccatatagag ttttaattgt tgtagttttg
    23341 atatttacgt ttttacaaac acttggaact ttgtatatac caacacttac agcaaatata
    23401 gtaaataatg gtgtagtaaa gggagatata gattatattg taaaaacagg tttaatgatg
    23461 atgatagtag ctggtataac agctttatca gctgtgttag tttgtaaagt atcagcaaat
    23521 ctttcatctg gattttgtag agatattaga gaagcagtat ttataaaatc acaagattta
    23581 tcaataaatg attttaataa tataggtaca gcatcaatga ttactaggag tactagtgat
    23641 attactttaa taggtcagtc tgtgtttatg tttattcaat tagtattacc agcaccaatt
    23701 ataactgttt ctggtctatt cttagcatat tctatagata aagcaatgac aattattatt
    23761 gttgtagtaa tgtttttatt catgctttca gcatttttag ttggtaaaaa gcttataaag
    23821 ttgtttaaaa tgatgcaaat aaagatggat aatatgaata gggtgttacg tgaggtagta
    23881 actggagtaa gagttataag agcttttaat aggagtcgtt ttgaaaaaaa gagatttgat
    23941 agaactgcaa tagattattc tgaaacagca atttcaataa ataaaatatt tgcagtactt
    24001 atgcctattg taatgttaat aatgaattta ggtatagtaa gtattatttg gtttggtggg
    24061 atgagagtca gtaatggaaa tatggaaata ggtcatataa tggcacttgt tgaatattgt
    24121 atcttaattt tattctattt aatcatggga gtaatggtat ttatgtacat acctagagca
    24181 ggtgcatgtg cagatagagt aaatcagata ttagatatag aacctgaaat tgtagatgga
    24241 aatggtcata aagatactgt ctcagagaga ggacatttag tatttaaaaa tgtaaccttt
    24301 tcatatgcac aatcagaaga acctgtgtta aataatataa catttgaagc aaaatctgga
    24361 gaagtaactg caattatagg aagcactggc tcaggcaaat ctactattgc aaatataata
    24421 ccacgttttt ttgaaattca atctggagaa atatctataa atggtcagga tattaagaag
    24481 attcctcaaa aagaacttag agataagatt ggatttgtac cacaaaaagc ttttttattt
    24541 agtggaacaa tagaagaaaa tataaggtat ggaaaagaag atgcaagtat tgaagaagta
    24601 aagcatgctg caagtatagc tcaagctgat gaatttattt atgatatgga agacaagtat
    24661 gactcatttg tagcacaagg tggaaataat ttgtcaggtg gtcaaaaaca gagaatatcc
    24721 attgcaagag cgttagttag aaagcctgaa gtatatgtct ttgatgatag tttttctgca
    24781 ttagatttta aaacagataa aagacttaga aaagcgttga aaaatgaaat caaagattca
    24841 tcagctataa ttatagcaca acgtataagt acaatcatgg atgctaatca gattatagtt
    24901 ttaaatgatg gaaaaattgt aggtattgga aaacataaag atttacttga aaattgtgaa
    24961 gtttataagc aaattgcgga ttcacaactt agtaaagaag aacttgcata aaatgaagaa
    25021 ttaatctgat atttggaggt gtatgatatg agtgaaaaaa gaatagatga acaagatata
    25081 gaagtactta gttttgaaga tggcatgtca gcagaaaaag ctaaggatgc aaaaaaaact
    25141 actaagagat tattaaaata tatggcaaaa caaaagttta aaatattaat tatttttatt
    25201 tcagtattga taagttcagc gcttacagtt ttagcaccta tggttatggg aaaagctatt
    25261 gaccaactat ttaatggaat aaaaactgca gtccaaacag gtacaaaatt cagtgtgaat
    25321 tttagtacta tgggagggat agtatcaata cttctagggt tgtacttaat tagtgcagta
    25381 tttatatata ttcaacagta tattatgtca ggagttgcac aaaatctagt tcttagcatg
    25441 agaaaagatt taagtgataa actcaataaa cttcctctta agtactatga ttctcataaa
    25501 aagggggaaa cacttagtat agtaacaaat gatttagaaa aagtagcaga tagtttacaa
    25561 gaaggattaa tgcaacttat tacagcagta gttacagtag ttggttctat tgtaatgatg
    25621 ataagtataa gtgtaccact tacaatagta tcagctataa cattacttgt ttcattgggt
    25681 attactgttg taattgcaag aaaatcacaa gaaagattct ctgaaaatca aaaggcacta
    25741 ggagagttaa atagcaatat agaagagata tttactggtc aaatagtggt taaagcattt
    25801 agtaaagaaa aggatactat aagaaacttt aaaatattaa atcaaaatct ttataatgca
    25861 agtagaaaag cacagttttc gtcatatgca atatctccaa ttattagatt tataaaccta
    25921 attggctatg ttattatagc agttgtaggt gggatatttg catcaactgg agcaatgact
    25981 cttggtagta tacaagcgtt tattcaatat gtaaatcaag catcagagcc tactacagaa
    26041 atatcttata ttgtaaatat gcttcaagca gcaatagcat ctgcagagag agtttttaca
    26101 gttatggacg aggtggaaga gataaaggat gaagaatctt caaaggttat tagcatgcct
    26161 aaaggtaaag tacaatttga gcatgttaaa tttggatata gtgatgattt tattcttatg
    26221 aaagatatta atatcaatct aaatgcagga gataagattg caattgttgg acctacagga
    26281 gctggaaaga caactcttgt aaatttactt atgagatttt atgagataca aggtggaaga
    26341 attactattg atggtgttaa tataaaagat ttaaagcgtg gagaacttag aactatgttt
    26401 ggtatggttc ttcaagatac atggctgttt aatggaagta taaaagaaaa tattgcatat
    26461 agtaagtcag atgccacaat ggatgaaatt gtaagagcag caaaatcagc aagagtagac
    26521 cattttataa ggactttacc acaagggtat gacactatat tgactgagga tgcttctaat
    26581 ctatctcaag gtcaaaagca acttctaaca attgccaggg caatactttc agacccatca
    26641 gtattgattc ttgatgaagc aacatctagt gtagatacta gaacagaggt agaaattcaa
    26701 aaggcaatga ataacttgat gaagggaaga actagttttg ttattgcaca tagattatca
    26761 actattcgag atgctgattt aatattagta atgaaagaag gaactattat tgaacaagga
    26821 agccataagg aacttattga aaagaaagga ttctatgaag aactttataa cagtcaattt
    26881 acaagtgaat atgatgaaga tgttgtatga ttatcaagtt ttaggcaact aaatgattga
    26941 taaatattaa gttaaatata gtaacatgta aggttatatg gaataagagt atttctctat
    27001 ttcatataac cttttttatt tatcaaaata agataaaatt tcctttctaa attagattga
    27061 agaatgtatt tatcaatata aaagtaaaat aatcattatt attagtgaag taaatattgt
    27121 ttgccacata tatgccataa agatgcactt attttacgga gatttaacaa agtagtgtaa
    27181 agtatactct aaatataaaa taaatcaaat agagttaaat aaattgagag gtgatataaa
    27241 tagtgaataa aaaattgaac tatagatttg aaatgaagca caaaataact gaagcagatg
    27301 tgttagcttt aaaatcaaga ctctatccaa taatgaagaa agatgaaaat gcatctaaag
    27361 atggaaaata tcttattagg agtttatatt ttgatactcc tgaagataaa gcacttttgg
    27421 ataaattaaa tggcgttgca ataagagaaa agtttaggat aagattttat aataatgact
    27481 gctcttatat aaggttagag aaaaaaatca aacattacaa catgacatca aagctatcaa
    27541 ctaatttaac caaaaaagaa gtaataaata tactaaataa tgatattgaa tttctaaaag
    27601 agtcgacaaa ttgtttatta agagaatttt atttgaaatt aaaatgtgaa agattagagg
    27661 caaaaagcat agttgattac aatagagaag cttttattta tccagttgga aatgtaagag
    27721 ttacaatcga tagtgatatt aaaacttctg taaattcggt agatttattt aataaagatt
    27781 tgccaactat ttcggtaatt gatgagaata tgactgtatt ggaggttaaa tatgatgaat
    27841 ttataccaga ttttataaag gatttaattc aaattaataa gactacctct acagcagttt
    27901 caaagtatgc ttcaagtaga ttatatattt aagaataaag gctatgattt atataaagat
    27961 atacataaat aaggttaaaa aacaaataag ttaaaaataa ataaaataaa aaataaataa
    28021 gttaaaaata ggaaggatga ttttaattat gacatttaat gatattttta aatcaagttt
    28081 tatagaaaac gtatcaggtt tttcatttgt ggacagtgct ttagcactag gttcagcttt
    28141 tttagtaggt ttgtttatat atatggtcta taaaaagact tatatgggaa ttatgtattc
    28201 tcgaccattc aatgtgtcgc ttgtagcact tacaatgctt acaacttttg taatattggc
    28261 ggttacttca aatgtagttt tatccttagg tatggttggt gctttatcta tagtaagatt
    28321 tagaacagca attaaagacc caatggattt agtatttcta ttttggtcat taggttcagg
    28381 tatagtttta ggagcaggtt tgattccctt agctgtcatg ggttcaataa ttatgggatt
    28441 gattctaata ttcttttcaa ataaaacaat atctgaaact ccatatatat tgatggtaaa
    28501 ttgtaataat gaagattcag aagatatagc aactgataaa attaaaaaag tatttactaa
    28561 atatcaaata aaatcaaaaa gtgttacacc agaaaaagga atagaattag tgtttgaagt
    28621 aagaatgaaa gatggagaaa caagtcttat aaatgacttg agtcaagtag atggagttac
    28681 taatgcagtt cttgtaagtt ataatggtga ctatgtagca tagagaggag taatctttta
    28741 tgaaagataa aaaatttacc cttcttatct cgattatgat tatattttta tgtgctgtag
    28801 ttggagttta tagtacatct agcaacaaaa gtgctgattt atatagtgat gtatatattg
    28861 aaaaatattt taacagagac aaggttatgg aagttaatat agagatagat gaaagtgact
    28921 tgaaggatat gaatgaaaat gctataaaag aagaatttaa ggttgcaaaa gtaactgtag
    28981 atggagatac atatggaaac gtaggtataa gaactaaagg aaattcaagt cttatatctg
    29041 tagcaaatag tgatagtgat agatacagct ataagattaa ttttgataag tataatacta
    29101 gtcaaagtat ggaagggctt actcaattaa atcttaataa ctgttactct gacccatctt
    29161 atatgagaga gtttttaaca tatagtattt gcgaggaaat gggattagcg actccagaat
    29221 ttgcatatgc taaagtctct ataaatggcg aatatcatgg tttgtatttg gcagtagaag
    29281 gattaaaaga gtcttatctt gaaaataatt ttggtaatgt aactggagac ttatataagt
    29341 cagatgaagg aagctcgttg caatataaag gagatgaccc agaaagttac tcaaacttaa
    29401 tcgttgaaag tgataaaaag acagctgatt ggtctaaaat cacaaaacta ttaaaatctt
    29461 tggatacagg tgaagatatt gaaaaatatc ttgatgtaga ttctgtcctt aaaaatatag
    29521 caataaatac agctttatta aaccttgata gctatcaagg gagttttgcc cataactatt
    29581 atttatatga gcaagatgga gtattttcta tgttaccatg ggattttaat atgtcatttg
    29641 gtggatttag tggttttggt ggaggtagtc aatctatagc aattgatgaa cctacgacag
    29701 gtaatttaga agacagacct ctcatatcct cgttattaaa aaatgagaca tacaaaacaa
    29761 aataccataa atatctggaa gagatagtaa caaaatacct agattcagac tatttagaga
    29821 atatgacaac aaaattgcat gacatgatag catcatatgt aaaagaagac ccaacagcat
    29881 tttatactta tgaagaattt gaaaaaaata taacatcttc aattgaagat tctagtgata
    29941 ataagggatt tggtaataaa gggtttgaca acaataactc taataacagt gattctaata
    30001 ataattctaa tagtgaaaat aagcgctctg gaaatcaaag tgatgaaaaa gaagttaatg
    30061 ctgaattaac atcaagcgta gtcaaagcta atacagataa tgaaactaaa aataaaacta
    30121 caaatgatag tgaaagtaag aataatacag ataaagataa aagtggaaat gataataatc
    30181 aaaagctaga aggtcctatg ggtaaaggag gtaagtcaat accaggggtt ttggaagttg
    30241 cagaagatat gagtaaaact ataaaatctc aattaagtgg agaaacttct tcgacaaagc
    30301 aaaactctgg tgatgaaagt tcaagtggaa ttaaaggtag tgaaaagttt gatgaggata
    30361 tgagtggtat gccagaacca cctgagggaa tggatggtaa aatgccacca ggaatgggta
    30421 atatggataa gggagatatg aatggtaaaa atggcaatat gaatatggat agaaatcaag
    30481 ataatccaag agaagctgga ggttttggca atagaggagg aggctctgtg agtaaaacaa
    30541 caacatactt caaattaatt ttaggtggag cttcaatgat aataatgtcg attatgttag
    30601 ttggtgtatc aagggtaaag agaagaagat ttataaagtc aaaataagat taaaattgaa
    30661 tataaataat tttaatactg ttaagtctaa atatttactt agcagtattt ttttagaaaa
    30721 taatgtattt ggttaataat ataaggtaat aaattgataa aaattaaata cagcatatta
    30781 ataatacata ataaagtaat aaaaaaactg tttaagtata ataacttata tttagacagt
    30841 ttttttcatt tataaataat ataaatgaaa ttgaaaacat ttgaaggata gaaattggtt
    30901 ggataaatgt ataaattact tcaccaaaaa ataatgacaa aaattgtaac taagttgtaa
    30961 taaaatggta gttatttagc ataaaataat gtatcataga agtatattat aaaattaata
    31021 aaataagatt tttgtaagaa aactgctggc ttttgatatt tagagtgagt agttaaatta
    31081 tgtttaggag gggtagaatt tgttgcaaaa agtattatgg aaatttttaa agctagtagt
    31141 cggtttattt atatgttcag ttggtatagt attgacgata aattgtaacc ttggattatc
    31201 tccctgggat gtatttcatc aaggactttc aaatcatata ggaataacta taggaactgc
    31261 ttctataatt gtaggctcta tagtagttat tgctgatgtc gttcttggag aaaatgtagg
    31321 atggggaact gtttttaaca tgctactaat tggattcttt atggatttac ttttatatag
    31381 taatttaatt ccagaggctg atagtttatt tgtaggaata attatgttga tattaggatt
    31441 ggttcttttg tctgttggaa tggtatttta tatgggaagt ggtttaggaa gtggacctag
    31501 agatggtttg atggttgcaa ttcaaaagaa aacaggaaaa tcacttaaat taataagagg
    31561 tactatagaa gttggagcat taatagtagg atttttatta ggtggaaaag ttggtatagg
    31621 aactataata agtgcatttg gattggggta tttcacacaa atggtatttg gcttatttaa
    31681 attagattgt agtaagatta aacatagatt tatagtagat gatataaaat ttatcaaagt
    31741 gtatatgagt ggtgagaaaa aagctgctgt tcaaaatata gttaagtaat ttaagaataa
    31801 attcaacgaa atgttaagta ttttaaatta acattttgtt tagatagata aattgaatat
    31861 taaaagaata ttaaaatatg aggattgcta gaacactata aaatatagat ttctagcaat
    31921 tttttatttg ttcaatattt ttttagtact agaagtctta ttctctattt aatgaatgaa
    31981 ttaatttata attagagata ctaaacttta tagatattaa tataaacacc tttaaactgt
    32041 gtattttcca aagcctttaa aatgcttgta tttattctaa ataaaaaaat aaaaaaatgt
    32101 caaaaaaact cttgtattat atccaaattt tatatataat tacctatgtt gtttagaaat
    32161 tttaaactca atgtttagta aacaaaatgt ttagtaaaat aaaacagaaa ctaggaggtg
    32221 caacatggat ataggtgaaa agataaaaag attaagaaca gaaaaacaac tgacccagga
    32281 agagttagct aatagatgtg aattgtcaaa gggatttatt tctcaattag aaaataactt
    32341 aacatcacca tccatagcta cccttataga tatacttgaa atactaggta caaatttaag
    32401 ggaatttttt aatgaaatag atgatgaaag aatcagtttt acaaaagaag atatgtttga
    32461 aacagaagat gaagatttaa aatataaatt aaagtggtta attccaaatt cacaaaaaaa
    32521 tgaaatggag ccaataataa taacattgta tccaggagga cagtataaag aagaaaagcc
    32581 acatgaaggt gaagaatttg gatatgtatt agcaggttca atatatgtcc atataggcga
    32641 aaagaaaaat aaagtaaaaa aaggagagag tttttacttt agaccgaaag caaatcacta
    32701 tatatcaaac gagggcaaaa ctacagcaaa agtgatttgg gtaagtacgc caccatcatt
    32761 ttagaataga ggtgtttaat aattggtaga aaatataata gaattaaggg atatatctaa
    32821 acattatgaa gaacttactg ttttagataa tttcaattta gatataaaga aaaatgagtt
    32881 tctaacatta cttggaccaa gtggatgtgg aaaaactaca acattaaaaa taatagcagg
    32941 atttgagtat gctgatgatg gtaaagtttt atttgaaggt aaggagataa ataatttgcc
    33001 accatatgaa agacaagtaa acacagtatt tcaaaaatat gctttatttc cacacatgga
    33061 tgtttatgaa aatgtagctt ttggattaaa aattaaaaag actccaaaag atgtaataga
    33121 tgctaaagta aaagaggttt tgaaacttgt tgcactagaa ggttttgaaa gacgacaagt
    33181 tgaatcattg agtggaggac aacaacaaag aattgcaata gcaagagctc tagttaatga
    33241 accaaaggta ttattactag atgaaccact tggagcactt gatttaaagt taagacaaga
    33301 gatgcaaata gaattaaaga gaatccaaaa aaaattagga ataacatttg tttttgtaac
    33361 tcatgaccaa gaagaggctc ttactatgtc agatactatt gttgtaatgt ataagggcaa
    33421 aattcaacaa atgggaacac cacaagatat atacaatgaa cctaaaaatt catttgtagc
    33481 taagtttata ggtgaaagta acatatttga tggaattatg atagaagatt ataaagttaa
    33541 tttctgtaat agagattttg agtgtgttga taaaggattc gaaaaaaacg aaaatataga
    33601 agtagtaata agaccagaag atataaaaat ggtaaagcca gaagaaggaa tgttaaaggg
    33661 gaaagttaca tctacagtgt ttaaaggagt gcattacgag atagaattga atgaaaatgg
    33721 aagattgtgg atacttcata atactaaaaa tgctgaggtt gggactgaac taggcatgga
    33781 tatatatcca gaagatatcc atataatgag aaaagagaat aactaagatg aaaagaaaat
    33841 cttttttagc atatccatat gtagtttgga gtgcgatatt tgtaataatt cctttaatat
    33901 tagtagtatt ttttagtttt acaaaagaat caggtggagg ttatgccttt acacttgaga
    33961 attataaaga agtaattgac cctatatata tgaaagtttt tgggcgttct attcttttag
    34021 caggaggagc tacattaata tgccttatag ttggttatcc agtggcatat ataatttcaa
    34081 aggctcgtgt aagtagaaga ggttcattaa tattattgtt tattcttcct atgtggatga
    34141 actttctttt aagaacatat gcatgggttg ctatattagg aaaaaatgga cttttaaata
    34201 catttttagg ttggtttgga atacaaccat tagcaatttt gtatactaat tttgccatac
    34261 ttttaggtat ggtgtataac tttttaccat ttatggttct tccaatatat acagcactat
    34321 caaagatgga taatgatttg ataaacgctg ctcatgactt gggtgcaaat aatatgacag
    34381 tttttagaaa aattatattt cctttgagtt tgccaggtgt aatgtctgga ataactatgg
    34441 tatttatgcc agcagtcact acttttgcaa tatcaagact gttaggtgga ggtaaaataa
    34501 tgctggttgg agatttgatt gaacaacagt ttacagttgt tggagattgg aactttggtt
    34561 cagctatatc gatatttatg atgatagtaa tattaatatc tatgtctata atgtcaaaat
    34621 ttggagatga atctgacaaa gaaggcggtg ggttactatt ttaaaattaa agaaaataac
    34681 ttctaatata tatttagcat tagtattctt atttttgtat gcacccatat ttgctttggt
    34741 actattttct ttcaatgatt caaaatcaat ggcaagatgg aatggattta catggaagtg
    34801 gtatggtcaa ttacttcaaa atgagagtat aatgagtgct ttatattata ctattgtaat
    34861 agctatatta gcgtctgtaa tatctacaat agtaggaact ataagtgcta taggaataca
    34921 taagatgaga ggtaagagta agaaattaat actaaatgta aactatcttc ctattttaaa
    34981 tacagagata gttacagcag tagctcttat gagtctgttt gtatttgtaa aaatggaatt
    35041 tggatttaca acaatgctat tggcacatat aatgttttgt ttgccatacg ttatactttc
    35101 agttttgcca aaaatgaagc aattacctga taatatagaa gatgcagcta tggacttggg
    35161 agcaacacct atatacgctc ttagaaaagt aattttacca caaataaaac caggtatagt
    35221 ttctggattt ttaattgcat ttactatgtc aatagatgac tttataataa gtttctttaa
    35281 tgctggaaat ggtgtaagca atctttctat agaaatctat ggaatggcta gaagaggtat
    35341 aaaacctgaa ataaatgcat tatcaacaat aatgtttgca gtggttctag gtttactttt
    35401 actagctaat aaaaaagaat ctatagtaag ggggattaaa taatgattaa gtttaaaaaa
    35461 gcaatatccc ttatagctgt aggtatgatg acagttactt tatttacagg atgtaataaa
    35521 gcaactgatt ctacaaaagt tttaaatgta tataatgttg gagattatat agatgagtct
    35581 cttatagata agtttgaaaa agaaacagga atagatgttc aatattcaac ttatgatact
    35641 aatgaaatga tgtatcaaaa agtaaagagt ggtagtacac actatgattt agtatttcct
    35701 tcagactata tggtagagaa aatgaaaaat gaaggtcttt tggaaaaatt agattttaag
    35761 aatataccta atatgaaata tttagataag agttttttaa atcctgtata cgacaaaact
    35821 aatgaatata gtgttgcata tatgtgggga actcttggaa tactgtacaa taagaaagaa
    35881 gttaaagacc ctatggatag ctggaatata ctttggaatc caaagtataa gggaaatata
    35941 atgatgtttg actctgttag agatactata gggataactc ttaaaaaatt aggatatagc
    36001 atgaatagtg taaatccaaa agaaataaat gaagctaaaa atcttttaat gaaacaaaag
    36061 gatttagtat tggcttatgt aaatgatgaa ggtaaggata gattgcttgg tggagaagtg
    36121 gcaatgggta tgctgtattc tggagatgca gttacactaa tggagcaaaa tcctgacctt
    36181 gattatgcta tacctaaaga gggaactaat aaatgggtag atgctatgtg tgtacctaca
    36241 acagcacaaa ataagaaaga agctgagctg tttataaact ttttattaga cccagagaat
    36301 gcaaaagtaa atgctgagta cattgggtat tcaacaccta atacaggagc actaaaatta
    36361 ttagacccag aaataactca gaatcctgtt gcatatccat ctaaagaagt gcttgataaa
    36421 tgtgaaactt ttgaagatat tggtgaaaat ataaaattat atgacaaggc ttggatagag
    36481 ttaaaatcaa aatagggtct tgttggttta agtaaattta cttaataaat acttaataaa
    36541 actgttcttt tttagaaaat ttattttcta attattgaac agttttattt tttgtttaag
    36601 ttatattcat atgcaattta aatagtttat taataaatga aaacttattt gtataatgac
    36661 tttaataata attagaaaaa atgggcaact taatagaata attggaggtg taaatttata
    36721 aaaaatgaat aggataaata aaaaaataga tttattaata ttggctttgc tcgtttttat
    36781 atttttaatt gttggaatag taatactatc gattaggaca gaaaataaat tgaatgtgtt
    36841 cataatgtta gcaataattt tttttataat aatgttaact tatttatcaa attcagtagt
    36901 ggggttgata acaagttcaa taattatatt tatgtataca tcctatattt tatataacaa
    36961 tattactcac aatatggatg ttgaatttat atcatatatg tggataatag caacaccagt
    37021 atcttcaatt ataatgggta atctgaacaa aagcataaat gaattgcaaa atacaaataa
    37081 aaaactatct gaacagtata aggaattagt gactattgat agtgaaactg gacttagaaa
    37141 tttaaagatt ttttataatg atgtaaatat ggagataagt aaatctatta gacataatac
    37201 tgatttttct cttatgatag taaaattacc atactacgga aacctacaga ctatatttgg
    37261 tgaaaacaaa actaataaaa tagtaaaata tattggttca aatattatag aatgtacaag
    37321 aaatgaagat ataatttata gtttacaaaa agatatgata gggatactaa tgccaaatac
    37381 atctttggaa ggttcaaaag ttgttaaaga cagaataaag aaaaggataa aagaattaaa
    37441 tttagattta aacaataggg gaaaatatgt aaatatagat gtcaaaattg catttttaca
    37501 atataagagt agttttggag atagtataaa ttttaagaat atagttgaag aggaacttca
    37561 atatgatgtt taaaatattt aataaaagtc tattaaaatt agtagttgta ttatcaatag
    37621 tactgacaat atcaaatttt agcttatctt catatgcatc agaaattact tccagcacag
    37681 ataaggtatt aatcatatat gattctaaga aagaaacagc ttataataga gatattttaa
    37741 atattatgag gactctattg ggaagattta gttcagatat agagctatta aaattgagta
    37801 actatgatgg tgagataaat aaaaattatt acagtcatat atttatttta ggaatcaatg
    37861 aaaactcata taataatgat aaaaacacta aaaacttgat tagttcctta aatttatata
    37921 aaggaactat atgttggtta ggatatggta tagaaaatct tttggaacat aaaaaataca
    37981 atttagacta tgtaggaaaa acaaacaata tagttagtgt aaattataga ggtaaatcat
    38041 ataacttgga tgaacattat gtttttaaca ttgtagaatc aaaagataca agtaataaag
    38101 taataggaag tattaatgat accttaaata agtatccata tataataaat gataaaaatc
    38161 ttttttatgt atcaaaatta gatttagatg gagttttatt ttatatattt tgcgattcac
    38221 ttaatgatat ttttaatatt aagacgtttg ataaaggacg tatatttgta agaatagaag
    38281 atgtacatgc ttttagagaa ccaaaaaatc ttgtagagat agcggattat ttaagtagta
    38341 agaatatacc atttacgatt gcattaatac cagcatatgt aaatcctaaa aatcataagg
    38401 taataacttt atcagaaagc cctgaaattg tcaaagcaat aaaatatatg caagataaag
    38461 gtggaactgt gatattgcat ggatatactc atcaatacaa gaaagaagaa gtttctggag
    38521 aagggtatga attttgggat ggtaaaaaag atgaaccctt aaaagaaaat atgaagatat
    38581 ttgtcaaaga tagagttttg aatggtctta gagtatgtat agagaatgga atttatcctc
    38641 ttgcatttga agcgccacat tatgcaatgg aatcagaggg atataaagaa ttaaaaaaat
    38701 atttttctac ttatataggg caacatcaaa ataatgataa aaaatttagc acaaatacat
    38761 atccttatat tattagggat acagaggagt ttaatatatt tatacccgaa aatttaggat
    38821 atatagaccc agaagataag tttacttttc aaaatataaa agaaaattta gataaattat
    38881 ccattgtaag aggttttagt ggagggttct tctttcattc atatttaaac atagagtatc
    38941 taaaaaacac aatagaatat ttagaaaaac agaatataga atttatgaat ttaagagatt
    39001 ttaataattg ggtaaaagtt gatgaaatac aaataagaaa taatggagat gaaattatag
    39061 tcaactatga taaggactta gatgaactaa ctaaaagtga tactagattt aaatccatat
    39121 ccaatattag taaagtttta atattcatag tatctataag tgttttaata tttgtgatta
    39181 tatttatata ttttaagaga atagataaaa agaaattttt aaaataaagg attttatatt
    39241 agaatcaata ttttaaaata ttttaggaga aatttatgag agatttaaat gtatcagagt
    39301 atttgttcgt atttagtttg tttagtatat ggtctttatt gcttataaat attattttag
    39361 ctatgggtgg atatatattt tattttaaaa actttgataa agaaataaaa gaaattgatg
    39421 aatatcctat gatatcaata cttgttcctg ctcataatga agctaaagtg ataggtagaa
    39481 ctgtggagtc tttgctatta ctcaattatc ctaagagtaa gatggaactt attgttataa
    39541 atgacaattc ttctgataat agtaaagaaa ttttagaaaa tataaaggat aggtacaaca
    39601 attataattt cactataata aatacagata gtttaacagg tggaaaagga aagtctaatg
    39661 cattaaatat tggatatacg atttcaaaag gagattttat agctgtttat gatgcagata
    39721 acacaccaga taaaaatgca cttagatatc tagtacaaac tatagtaatg aatgatgaat
    39781 taggtgctgt tattgggaaa tttaggactc gaaataaaaa taaaaaccta ttaaccaaat
    39841 ttataaatat agaaacatta agctttcaat ggatgtcaca agctggtaga tggcaacttt
    39901 ttaatctatg tacaatacca ggaactaact ttatattgag gagaagtatc atagaagaaa
    39961 ttggaggatg ggatagtaaa gcaattgcag aagatacaga aataagtttt aggatatata
    40021 aattgggata caagataaag cttgtaccac aatctataac atgggaacaa gagccagaaa
    40081 ctgttaaggt gtggataaag caacgtacta gatgggcaaa agggaatata tatgtattaa
    40141 tgaaatatat aaaaaatata tttaaacagg gaagaaataa gattgtgttt gatatagctt
    40201 actttttttc tgtatatttt ctatttttaa catctgtaat tatatccgat attttatttg
    40261 ttttaagtat atctaaacta gtagaaattt ctattccaat taactttttt ctaatatgga
    40321 tactatctta tcttttattt attatagaag tgagtataag ccttactata gaaaaaggtg
    40381 aagcaacaat tgaaaatata ttcatagtgg caataatgta ttttacatac tctcaactat
    40441 ggctgtttgt agctataaaa ggaatgatag agtatctaaa agatataatt tttaaaagag
    40501 aggttaaatg gtataaaaca gaaagatttt gattcatgta caggaggaat taacatgaaa
    40561 aaatttatta tatcaattat atctttagta ttgttttttt caaatatatc acttatatac
    40621 aaagtaaatg ctgatgagac taaagttaaa aattataagt ttgaaagaga tataactatt
    40681 gatggtgtta taggaagtaa tagtactttt tttgaagtaa ataaaaattg ggatattgaa
    40741 gaagttttat tgcatcttaa ttttagtaag agccaaatat taaatggaga tgtttcaagt
    40801 ttaactgtat tgataaataa tgtaccaata aaatctatta aattaaatgc taagacaaat
    40861 tataaaaata cattagaagt attagttcca aaagattata taattcaagg atataatgaa
    40921 ataaaaataa aaacatacaa aacaatatct gataaaattt gccaagatga ttccaatact
    40981 gggaactgga tggttattca caaagaatca tatatatcaa ttagatataa gcagaaaaag
    41041 gtggaaaact caataaatga atatccgtat ccatatgcag aaattgaaaa taatcataaa
    41101 ttagatacaa ctatagtagt accagataat atgactagag gagagactac agcagtgttt
    41161 aatttggcat cagcatttgg aaaaataaca aaaaatgatg atttaaagtt agatgttaaa
    41221 ttatattcag aaatgaaaaa ttggtcagat gataatatta tatatattgg taaacctgaa
    41281 aatacagcag aagaaatttt ggatatttta agtataaagg aacaaaccct attatcttca
    41341 aactgtataa tcaagcaagt agattcacca tacaataaga ataagaagat gatggtggta
    41401 attggtagta atgaagatga tttaataaag gcatcaaatt tattaataga aaatagactt
    41461 tctaaccaag ttttatcatc atcagtactt gtaaacaaag agactaatat aaaaattaat
    41521 agggaacaaa aattaaattt aggtcattta acattaaaag atttaggata ttctgacttt
    41581 ttgttggaag gagcatttaa tcaacaagct ttgtttgatg tcaaaatacc aacgggtaag
    41641 gtattagatg atggttcaaa gattatctta aatttaagat attctgacaa tttagatttt
    41701 gaaaaatctt tagttacagt gtctataaat gatgttattg tgggaagtaa aaaattggat
    41761 aggtcacatt ctaataatga taagttagaa ttaaaaatac ctaaagatat agataataaa
    41821 aactattatc aagttaaatt gacatttaat ttgagtataa aaaattcgaa ttgtgttact
    41881 agagagagta ataatccttg ggcatatgta tcaaataatt cttatttggc gttatctacg
    41941 aaagaaaatg aaaccttatc ttttgaaaat tatccgtatc catttgttag agatgatgaa
    42001 tttaatgatt taactgtgat aatgccagat tattcagggt cacaagctat gacctggatg
    42061 tttagattag gtgttacttt aggtgcgaat ataaattcac ataatggaaa tataaatgta
    42121 attagaggaa aagaatttag tgacaagtat aaggatacta atattgtagt ttttggagtg
    42181 ccacataata attctgttat aaagatgtta aataataatt taaatataaa gttcgataaa
    42241 aattactcca attttatatc aaatgataag ataagcttta ttgatgatta tggaaaaaat
    42301 atttctacta ttcaattaat aaaatcacca tataataatc aaaaaaatat catggtaatt
    42361 agttcaatga atgaaaaaaa cttatattta ggaatggatt atttgttgaa taaaagtaaa
    42421 gttaatgatt taaaaggaga tacattgata attgatgaat acggagaggt agaagattta
    42481 gcttataatt taaaatctaa gaaagaagta aaagattcaa gttggaatat gagtataaat
    42541 aagacaacta aagtattttt aatgatttct tttattacta taattatagt catgatatta
    42601 tcaatgctat atataaaaaa atataaaaga agataatatg ataaatttat tattttaaaa
    42661 gaggtatata atttgaggaa atatatgaat gaatttaaat taaaaagaag tattttaatc
    42721 tcattaataa tcttaatttt tatatttata gtttactttg taagtattga gttaaaagat
    42781 aggagtctag taaataatca atataaaggt aagattaaat cggccaattt atctgtagat
    42841 tatgaaataa atcaagttat gaaggatata gataaattgg gattgaatac agtaaatgta
    42901 cctataatta taaatgtaga gtccattaat tctgatatta tgtcaataga taataatagt
    42961 aaagaaaagg caattaagtt aataaaaaaa ttaaataaaa aaggtatttc aacaatatta
    43021 gaggcatatc catggattga aaatggaaaa ttgtatgaga ctgactggaa ccctataaat
    43081 aaaaaaagat ttttttatac ttggcaaaat gccatattaa atgaacttat aattgatgta
    43141 gcaaatccac tggatgtaaa tgttttaaat attggttcta attttgtaca tttggaagaa
    43201 tatcaacaaa attggggtga aataatagat tttgtacaat ccaaatttga cggtcttgta
    43261 acgtacagaa ctaattggtg gtatacaaaa aaagatgatt taagtagtaa gttgttttat
    43321 gaacaaaagc tgaacaataa tttttttgat aagttagatt ttatatctat agcagcatat
    43381 tttgagttaa gtaataaacc tgttaataca gtggatgaat taatttctgc cttacactca
    43441 tctactgtaa acaatagagg acaaaatata aaacaagaaa tatataacct ttataaagct
    43501 catagaaaac ctatattttt tggagaatta ggtttttcaa atagagaaag tgcaagttct
    43561 cagccgtgga atcatactcc ttcaaaagct gtaaatggcg aagaacaagc aaggtgtttt
    43621 gaggcatata agaaagtttt tgaaaatgaa gactggataa atggtttttc tgtgttttgt
    43681 gtaggtaaaa tagatgacga aaaaaatttt tacccatcaa aagagagtat aaaagttatt
    43741 aagtcttggt atgaataatt ttgaaagtgt agaatttata tagattaaat agatgattag
    43801 gagagatata aatgaatgac atcaaggtta tgactgtatt tggaactaga cctgaagcta
    43861 tcaaagtagc acctttaata aaggaacttg aaaaaagaga gaatataaaa agtattgttt
    43921 gcgttacagc tcaacataga gaaatgcttg accaagttat agaaaccttt aatataaatg
    43981 tagactatga tttggatata atggaaaaag gacaaagcct gaatgatata acatgtaaga
    44041 ttttaaataa acttcccttg attttaaaca aggaaaatcc aaatataata ttggtacatg
    44101 gagatacaac cacaactcta gctacaagtt tgactgcatt ttataataaa actcttgttg
    44161 gtcatataga agctgggctt agaacctatg ataaatattc tccttttcca gaggagctaa
    44221 atagacaact tacaggtatt attgctgata tgcactttgc tcctactaat ttggctagaa
    44281 aaaacttgat ttctgaagga aaacctaata ataatatatt tgtaactggt aatactgcta
    44341 ttgatgcttt aaagatgaca ataaaagaaa actataatca tcctataatt gatgagattg
    44401 gtaacgatag gatgattttg ttaacctctc acagaagaga aaatttgggt aaacctatga
    44461 aaaatatatt cagagctata aagcgaatag tagatgactt tgaagatgta cagatagtat
    44521 atcccataca tttaaatcct aaaatacgaa ctatagctga tgagatattt ggaaaatttc
    44581 ctgaaaaaat tcatattata gagcctttag atgtagctga ttttcataac tttttaaata
    44641 aatcttatat gataatgact gatagtggag gaatacagga agaagctcca tcattaggta
    44701 agccagttct tgtgttgcgt gataaaacag aaagaacaga aggaattgaa gcaaagacac
    44761 ttaaattagt aggtactaat gaagatagaa tatataattc agtgagcgat ttattaataa
    44821 ataaagataa ctatgttcag atgagtaaag catctaatcc atatggagat ggaaatgcaa
    44881 gcaaatatat agtggatata ataattaaaa aatttaattg caaatattta aattagataa
    44941 ggcctaaaac attaactaat tgatataaga tttatgataa tattaaggaa tacaattttt
    45001 aagggggagt aatatggcta gaaaattaat aaaaaatttg ggtaaaagta aaagtgtaaa
    45061 gagagtgaaa cttttattta agaagatttt tattactgta ttcatagtag caagtatagt
    45121 tgctattttt aatataacga aatattttga ggaattatac aaagtaaggg atttaaaaag
    45181 tactaaaatt gaatactata tggatgtggc tgatgaagca ggagatggaa aggtccaatt
    45241 aagttggaag gccttacttg ctatagacat ggtaatacat gatgaagatt taagtaatat
    45301 aaaaaagaaa gacacattgg atatagggga aaagtttata gtagaagata aaaatggtaa
    45361 aggcgaaaag gtgtataagg taaaaaagtt taataaggta ttaagcgaat tgaaatttga
    45421 ctcttctcaa aaaagtagag caagaaaata catgaaagat ttagaataca cataccttgg
    45481 aaataaacaa ttagatagta gtgatgaaaa aattaaattt ataaagaagt tagaagactc
    45541 agctataaga gaatatattg attatggaat attgccctct ataacaattg gacaagctat
    45601 attagaatct ggttggggaa attctaaact tacaaaacag agtaataatt tatttggtat
    45661 aaaagcagat aaagcatgga aaggaaaaag tgtagaaatt tcaacttcag agcattataa
    45721 tgaaaaaatt gtagctagtt ttaggtcgta taattcatta caagattctg tcaaagacca
    45781 cagtttattt ttaattaata ataaaagata tagaaaacat gggttgtttg aggcaaaaga
    45841 ttatattagt caagctcaag cattagaaaa tgcaggatat agtacagcag aagataaaaa
    45901 aggaaatcgt atatatgcag agctattaat agatgttatt aggagttata atttgcaatt
    45961 gatagataat aaagttgaga caaaataatt gttaacttta tatgaggaga taacaagata
    46021 agattttttt ctacaaaaag aatagaaaaa ttgtaaaaaa aactaaaaaa atgtattgac
    46081 ttgttattta aaaggtgata ttattattac tgtactcgaa agagaacaaa a
//
LOCUS       DAMJYR010000018        45491 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.018, whole genome shotgun sequence.
ACCESSION   DAMJYR010000018 DAMJYR010000000
VERSION     DAMJYR010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 45491)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45491)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45491
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.018"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            79..738
                     /gene="cprR"
                     /locus_tag="PC471_000969"
     CDS             79..738
                     /gene="cprR"
                     /locus_tag="PC471_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CprR"
                     /protein_id="HDF2788108.1"
                     /translation="MSKILVVDDELDMLKLIENVLKRDGHEVKVISDVEKAMNMDFNY
                     FNLIILDVMMPKMDGFEVCKTIRNKVDCPILFLTAKNMESDIMYGLGIGADDYITKPF
                     GVGELRARITAHLRRESREKKNLLTISNVQFNFLGKEVSIDGKKINFTKSEYLICEFL
                     AKNKGQVFSKERIYESVYGFDGESDISTITEHIKNIRSKLKQYDIYTIETVWGIGYKW
                     V"
     gene            949..2007
                     /locus_tag="PC471_000970"
     CDS             949..2007
                     /locus_tag="PC471_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HDF2788109.1"
                     /translation="MDRLKNIRKSLLQYTFLFLLIGITIYLVFKTLDIKMLSNVMVMV
                     DKKFLFIGALAIMCHIMLEGVVMKIIIESTHKVNIRFIGFKLAIMGFYYNLITPFASG
                     SQPVQIYVLKKCKMPLSKASAVVTNKSIIFQIVVTFYCTILVLMNFAMLEKQMKMIMP
                     LVFLGIAINSFVLIMIILVILNPEKIKYFMRITIKHLSKIKFLKFLDNKIESIESFID
                     DYSKAINFFAKNKKVLISTIIVTFIQLSAYFSVSFWIYKAFNLQGYSYIYILTLQAFL
                     YMAISPIPTPGNVGANELAFFTIFKSVFPQPLMGYAVFLYGGFMYYLILIGSGIFTVI
                     THYRMKNRIGRNIVLSEN"
     gene            2258..4402
                     /gene="fdhF"
                     /locus_tag="PC471_000971"
     CDS             2258..4402
                     /gene="fdhF"
                     /locus_tag="PC471_000971"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:2672..2674,aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HDF2788110.1"
                     /translation="MEKKILTVCPYCGAGCQLYLVVKDNKILRAEPANGRTNQESLCL
                     KGRYGWDFLNDPKILTPRLKKPMIRKQGVLEEVEWDEAINYTASRLNEIKEKYGPDSI
                     MGTGSARGPGNEANYIMQKFMRATIGTNNVDHCARVUHAPSVAGLAYSLGSGAMSNSI
                     PEIENADVLFIFGYNGADSHPIVANRIVKAKKNGAKLIVTDPRVTESARIADIHLPIK
                     GGTNMVLVNAFGNVLIEEGLYNKEFVQNHTQGFDEYKEIVKPYTAKYAEKITGIPEEL
                     IRKAMREYAKGKKAMILYGMGVCQFGQAVDVVKGLASIALLTGNFGRESVGIGPVRGQ
                     NNVQGACDMGALPNVYPGYQNVTDDKIREKFEKAWGGKLSPNNGYSLTQVPNLVLKEK
                     KLKAYYIFGEDPVQSDPDASEVREALDELEFVIVQDIFMNKTALHADVILPATSWGEH
                     EGVYTCADRGFQLMRKAIEPQGDVKPDWQIISEISTAMGYPMNYKNTKEIWDELRQLC
                     PSFLGATYEKIETQGCVQWPCKSESMEDKGTMYLYEGQKFSTPNGKGNLFAAEWRPPM
                     EVEDDEYPFSLCTVREVGHYSVRTMTGNCRTLSSLEDEPGRVQINSNDAEKLGIEDDE
                     LVRISSRRGSVITRATVTDRVKEGATYMTYQWWVGACNELTIANLDPISKTPEYKYCA
                     VKLEKLEDQELAEKCVREEYQSLKDKMTATNI"
     gene            4451..5284
                     /gene="fdhD"
                     /locus_tag="PC471_000972"
     CDS             4451..5284
                     /gene="fdhD"
                     /locus_tag="PC471_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HDF2788111.1"
                     /translation="MNNIYSKTKLQENSKDKVFENSYSLVDYKYLNSFNVEVSKINEH
                     EVSIDNEEIIAEYPLSFILNDKYVNTFLCTPYNLEELIVGFLKTKGYIENKKSLLKFE
                     INESERFAKVTIQEKQKNQDEQIIFLNSLDYITCAPVKSNIKINYDTIYSIMDKNLNS
                     SELFKNTGGVHSVSVFNFDKKIVTCEDVARHNAMDKAIGFCVLNDITLKDKIVAVSGR
                     ISLEMILKAAMTQIPIVVSKSAPTNLSIELSKKLNITLIGFVRGRRMNIYANPQRVIK
                     K"
     gene            5295..5468
                     /locus_tag="PC471_000973"
     CDS             5295..5468
                     /locus_tag="PC471_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2788112.1"
                     /translation="MCTKKEEQRYSKCEICGVLIDDTDKVYNNTYNLCKKCKRNISTE
                     KIVKYINIYDESK"
     gene            5526..6077
                     /locus_tag="PC471_000974"
     CDS             5526..6077
                     /locus_tag="PC471_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HDF2788113.1"
                     /translation="MKNKIGSFVIADPNKCTGCRACEVACFTMHNQHNNVGYTVGTVD
                     IPVIPRLYLVKGDNFCMPIQCRHCEDAPCLNSCPQKAIVKENNIMSVNEEKCIGCKTC
                     LLACPFGAIDLLPQYEDGKEVEQVILDENKKIAYKCDLCKDNEKIACISACPQEALKL
                     VTPIDDKKAKNRQAALSLLLTNK"
     gene            6102..7478
                     /locus_tag="PC471_000975"
     CDS             6102..7478
                     /locus_tag="PC471_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron hydrogenase small subunit"
                     /protein_id="HDF2788114.1"
                     /translation="MIINIDKDLCTGCRECSKVCPVNAIEGEEGKPQEINLDRCVMCG
                     QCVQTCKSYASVIDEGFEFLQEKKQEREIPESINEPIFAAYNVCDIDKVKKALSDPNV
                     FTMVQCAPAVRVALGEDFGYYLGYLGAGKMAAALRALNFDRVYDTNFAADLTIMEEGS
                     ELIKRVTEGGTLPMFTSCCPAWVKFMEKNYPKLTNHLSSCKSPQQMGGAIFKTYGAEI
                     NNVDASKIFSVSIMPCTCKKYECDREEMDSSGYRDVDVVLTTRELAYLIKDMGIDFKN
                     LREEKFDSPLGSYSGAGTIFGVTGGVMEAAIRTGYELITGESIPDVEVKQVRGENGFR
                     KSTIKIGDLDLRVGVVSGLKNVIPVLEDLEKGKLDVDFIEVMTCPVGCVSGGGQPKIL
                     LEEYKELAYENRTKATYVHDEHLAIRKSHENPDIKKLYDEYLVEPLGEKSHHLLHTTY
                     CIGKEVAK"
     gene            7478..7993
                     /locus_tag="PC471_000976"
     CDS             7478..7993
                     /locus_tag="PC471_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HDF2788115.1"
                     /translation="MNYFVIADPDKCIGCRTCMIGCVVAHSDEDIFYQNPDEINFNPK
                     LSVIKTKEVSAPIQCRHCEDAPCAKACPNGGIVRVGNTIKINEENCIGCKTCMLACPI
                     GAIDIVTLKDVDEGKLCFRERMTANKCDFCIESPEGPACVNVCPTKAFTIVKDEDIDS
                     KVKNKRKLAIL"
     gene            complement(8071..9354)
                     /locus_tag="PC471_000977"
     CDS             complement(8071..9354)
                     /locus_tag="PC471_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugated bile salt MFS transporter"
                     /protein_id="HDF2788116.1"
                     /translation="MNTLLNNKFSIKNFSNAWVIVLGCMLIQAVPFSIVASIQPQFIS
                     YVVEGEGFSLASFSLIFTIGTIASAIASPFIGVLFSKVNIKILYLLGCIISSIAFATF
                     SICTELWQFYTVSCIVQIGATIISAIGIPVLISAWFDEESKGKALGFAFAGGSIGSIF
                     LQPLAVRLLATQGYSRSYLIFGIIALIVGIPVILFILRMPKDINEKVKSKGKKEKASD
                     TISPSVWGYTLKEAKKIKYFWIMTIGFIFVGIYVSSLSVQYPTYLRNYLNPILIGTVG
                     STFALFALIGNLFGGLLFDKFGQTKPLIISFVLVLIATLSLLLVDKSSTFAFVFAILK
                     GLSVFAYMIGPAYLTGSFFGQKEFGSIYGIVQLIFAVGFALGSTLFGLLVDNFGYRFS
                     WSIIILSVIIAYTLLIITSIGMKKLNKERLDKLMQ"
     gene            9542..9964
                     /locus_tag="PC471_000978"
     CDS             9542..9964
                     /locus_tag="PC471_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3783 domain-containing protein"
                     /protein_id="HDF2788117.1"
                     /translation="MSFEKINNNNLDKIHNRKCIMLVNFNKKELSLIKNVSNFAGIRD
                     HIILSDKNGENIIRDILDGNISNECENGIKSKSVIFNNTPSTQVNAFLEGLKKVRINR
                     PFTAMVTETSIDWTLNNLIENLVQERNAMSLGKTIEHK"
     gene            10066..11004
                     /locus_tag="PC471_000979"
     CDS             10066..11004
                     /locus_tag="PC471_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HDF2788118.1"
                     /translation="MKVLFTINYGKEKFDKIRELGYDVIHYSEDSIENNEDVNTADVL
                     VTYNPFERLDISKMKNLQYIQTTSVGIDQLPKEEVLKRNIKIANNRGGYSVPIGESIV
                     LYILEIYKNSAKFFKQQQNKEWKMDFSVSELYGQRVGFLGTGTISSEAAKRLKAFGVE
                     IWGVNTDGSSKKYFDKCFATEDMNIVFEECDIVVVAMPSTKSTDGIVNKDMFKLMKDG
                     SVFINVGRGNTVNQKDLEECVEKFRGVALDVFESEPLNKDNKLWECENVIVTPHNSWV
                     SDKNKERTFNMVYNNLKHYIMENKPVDNVVDISRGY"
     gene            11190..13214
                     /gene="ligA"
                     /locus_tag="PC471_000980"
     CDS             11190..13214
                     /gene="ligA"
                     /locus_tag="PC471_000980"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HDF2788119.1"
                     /translation="MSVKDEINKLIETINYHNERYYNQDSPEISDYEYDKLMKELISL
                     EEEHPELKRIDSPTNRVGGKPLDKFNQIVHKTPMLSLSNAFSEQDLRDFDKRVQEYVG
                     ENVEYVVEFKIDGLSVGLTYNNGEFEKGATRGDGVVGEDISQNLMTVKSIPLKINDKK
                     ELIVRGEVYISKDNFRKVNEQQEEQEQPLFANPRNLAAGSLRQLDPKLTAKRPLDIFV
                     FNMENIEEYDLESHSESLEYLEKLGFSVSQSYKVCKSIDEVIEHIEYWTDNRGNLDYE
                     IDGMVIKVNNIKQRDEMGYTAKSPRWAIAYKFPAEKKKTKIVDIIVEVGRTGTITPSA
                     ILEPVRLAGTTVSRATLHNEDYIREKDIKINDTVLVQKAGDIIPQVLEVIKEERTGDE
                     IDFKMPEKCPVCSEPTIRLEGEAAVKCINMSCPAQIRRGIIHFVSRDAMNIDGLGESI
                     ITLLLNEKIIQDVSDLYYIKKEDVVDLERMGEKSADNLINSIEKSKENDLWRLINGLG
                     IKFIGVKAAKILAYNFKDLDEVISATSEQLEELEEFGSIMANSVVEFFKEDKNMNVIN
                     KLKNVGVNTKSLDNTGEKVEKIFEGMKIVLTGTLPTLKRNDAKDMIEKRGGKATSSVS
                     KSTTFVLAGEEAGSKLTKANDLGIKVIDEERFLELLKLSSKDEVKAVLES"
     gene            13374..14723
                     /gene="rph"
                     /locus_tag="PC471_000981"
     CDS             13374..14723
                     /gene="rph"
                     /locus_tag="PC471_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HDF2788120.1"
                     /translation="MTRSYDRSNEQIRPVKITRDYTKYAEGSVLIEMGETKVICTASV
                     EEKVPPFLRNSGTGWINAEYSMLPRATQQRKIRDASRGKIDGRTQEIQRLIGRTIRSV
                     VDLNKLGERTIWVDCDVIQADGGTRTASITGAFVAVAEAIYKLYKAKTIKEMPITNFV
                     SAISVGIVNGIHMLDLCYEEDSKAQVDMNIIMTDKCEFVEVQGTGEEKPFSRADLNLL
                     LELGEKGNKELIRLQREALGEISDEILGMDYGNEVVIATNNAHKLEEIGEILKDFEYK
                     VYSLKDVDLAGIEIVEDGKTFEHNALIKARTIAKKTKLIAISDDSGLEVDALGKKPGV
                     YSARYAGEHATDEENRKKLLKAMQNVPMSKRNARFVSAIAVVFPDGKEFVVRGICEGM
                     IGFEEKGKNGFGYDSLFIVKGYDKTFGEIPSVIKNSISHRANALKLMKQEFIKRVVK"
     gene            14723..15193
                     /locus_tag="PC471_000982"
     CDS             14723..15193
                     /locus_tag="PC471_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HDF2788121.1"
                     /translation="MKIGIVSDTHMMIKNMEKTIPYLKECDLIIHAGDNFTDSRYIHS
                     MTNVGIIAVKGNCDFDAVEEEVVFEVANKTIFLCHGDKYGVKYGTNMLEKKATEVDAD
                     IVIFGHTHTPFREIKDGVLYINPGSTSLPRGVSYKSFVIMDIEEDDIKIEEIRI"
     gene            15387..16673
                     /locus_tag="PC471_000983"
     CDS             15387..16673
                     /locus_tag="PC471_000983"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HDF2788122.1"
                     /translation="MKAELIKKEGNKVTLKITVDNNKFEAAVNKAYNKSRNKYNIPGF
                     RKGKAPRIVIETQYGKGIFYNDAIEILFPEVYPEAIKELDIDPIDNPDLDIEEISKDN
                     GLVMVLNVEVKPEFELGNYKGIEIAKVENTVSDENVDAKLQEMVEKNARLVSVEDKAL
                     EDGDTAIIDFEGFENGVAFDGGKGENYNLVIGSNTFIPGFEEQLVGKKAGEEVEVNVT
                     FPEEYHSQDLAGKPVVFNVKINDVKVKELSALDDEFAKDTSEFDSLDELKADVRAKLE
                     EEAKNRADAETRNSVVEKVAENTEIEIPEVMIQHQIDNMLNELNYQLQYQGFGLQQLL
                     EMTGRTMEELREERKEDAKKLVKSSLVLEAITKAEGIEATEEEFKAELEKMASAYNME
                     VEKIEASLRDADKEDIKGQIKIRKTIDLLVDNATIA"
     gene            16823..17407
                     /gene="clpP"
                     /locus_tag="PC471_000984"
     CDS             16823..17407
                     /gene="clpP"
                     /locus_tag="PC471_000984"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HDF2788123.1"
                     /translation="MALVPVVVEQTGRGERSYDIFSRLLKDRIIFLGDQVNDATAGLI
                     VAQLLFLEAEDPDKDIHLYINSPGGSITSGMAIYDTMQYIKPDVSTICIGMAASMGAF
                     LLAAGAKGKRLALPNSEIMIHQPLGGAQGQATDIEIHAKRILKIKETLNEILSERTGQ
                     PLEKIKMDTERDNFMSALEAKEYGLIDEVFTKRP"
     gene            17426..18676
                     /gene="clpX"
                     /locus_tag="PC471_000985"
     CDS             17426..18676
                     /gene="clpX"
                     /locus_tag="PC471_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HDF2788124.1"
                     /translation="MSKYEEKRQLKCSFCGKNQDQVRRLIAGPNVYICDECVELCDEI
                     IQEEIEDTIDEDTTSLPKPKEMMEILNDYVIGQEKAKKALSVAVYNHYKRIYSKKSSS
                     KDIEIQKSNILLLGPTGSGKTLLAQTLARTLNVPFAMADATSLTEAGYVGEDVENILL
                     KLIQAADFDIEKAERGIIYIDEIDKIARKSENPSITRDVSGEGVQQALLKILEGTVAN
                     VPPQGGRKHPHQEFLKIDTTNVLFILGGAFDGLEKIIQKRGGDKTLGFGAKIESKKEL
                     DLGKLYEKVLPEDLLKYGIIPEFIGRIPVLATLELLDEDALMQILQEPKNALVKQYKK
                     LLELDDVELEFEEGALRAIAKKAIERNTGARGLRSIVESVMMETMFEVPSRDNIKKVI
                     VTEKSVNEDSVNPIIVLKDQEESA"
     gene            complement(18747..19274)
                     /locus_tag="PC471_000986"
     CDS             complement(18747..19274)
                     /locus_tag="PC471_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HDF2788125.1"
                     /translation="MSEIILLFLIFLKIGAFSFGGGYAMLPFIQQEFISKYHFITNQE
                     FLDLLAISQSTPGPVAINSATFVGFKTAGFFGSLAATIGVTIFALIGLTIISKLFSKF
                     KNNKKVENLLTVLRPITLGFILSAAFSSAKEVNWDLYGIIAFIASFYLLYKGKIGSIA
                     IVFVFGILGILLTFL"
     gene            complement(19262..19774)
                     /locus_tag="PC471_000987"
     CDS             complement(19262..19774)
                     /locus_tag="PC471_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HDF2788126.1"
                     /translation="MLKLFLTFLKIGAFTFGGGYAMVPLIQDELVNKQKLLEQEEFID
                     YLSIAQSYPGVLAVNISVLLGYKLYGIPGVLICTLGSALPSFCIVLVLSYLYFKNSSS
                     KILDGFFKGVAPVVIALILYSFTSMFKKLPKSKTNILLLIIAVLSVGVFNISPIYLII
                     GGGVYSLCQK"
     gene            19954..22317
                     /gene="lon"
                     /locus_tag="PC471_000988"
     CDS             19954..22317
                     /gene="lon"
                     /locus_tag="PC471_000988"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HDF2788127.1"
                     /translation="MEQNYTKIDHELPLIPLRGLAIFPYMILNFDIGREISLKALDQA
                     MMDEELIFLTSQKEAEVDEPGEEDFYHVGTICKVKQMIKLPGDTVRVLVEGVSRGRVK
                     KIEQEDGYFRAVIEEIVFDSDNLDSETEVEIEAFVRNVFDAFEEYINIGNRVSPEILI
                     SLADIEDVDRFIDTIAANIYLKSSQKQEILEEFDIRKRLELIYSILLEEIDILKIEKK
                     ITLRVKKQMNKVQKEYYLREQLKAIQKELGEEEDINSEADEYREKLKKIKAPKTTKEK
                     IEKEIDKFSKISSMSPDVSVSRNYLDTIFSLPWNKETKDKLDITKAKDILDEDHYGLE
                     KVKERILEYLAIRTLAKSLKGPIICLVGPPGTGKTSIVKSIARALNRKFVRISLGGVR
                     DEAEIRGHRRTYVGSIPGRIINGVKEAQTKNPVFLFDEIDKMAADYKGDPASAMLEVL
                     DPEQNKDFVDHYLEIPFDLSKILFVTTANSLSNIPRPLLDRMEVIEVSGYIEEEKLNI
                     AKKYLLPKQIKEHALKENFIKIDDETLRSIINHYTREAGVRTLERTIGKICRKVAKKY
                     VEDPTLEEVVINKSDLETYLGKDMFKYQLAEVNPQIGLVNGLAWTEVGGVTLEVEVNV
                     LKGKGEIVLTGKLGDVMKESAKTGISYIRSIVDKFDIDPEFYKTNDIHIHIPEGAVPK
                     DGPSAGITMALAVISALTKRPVPGNIAMTGEITLRGRVLAVGGVKEKLLAAHRAGITK
                     VLIPKECEADLDEIPENVKEKMEFVLVEHMDEVLEQALLKSGENNEN"
     gene            22307..22918
                     /locus_tag="PC471_000989"
     CDS             22307..22918
                     /locus_tag="PC471_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HDF2788128.1"
                     /translation="MKIRSSEITMSAVNKSQYPAEGIPEIALAGRSNVGKSSIINTLL
                     NRRNFARTSQTPGKTRTINFYLINNEFYFVDLPGYGYAKIAKSEKEKWGGIMERYLES
                     RQELCSIFLLVDIRHEPTADDKLMYEWIKHFGYNCVVIATKADKISRGQYQKHISIIR
                     KKLQMESSEKVIPVSSLKKTGVEELWEEIVNQYNQHGYEITVD"
     gene            complement(23183..24553)
                     /locus_tag="PC471_000990"
     CDS             complement(23183..24553)
                     /locus_tag="PC471_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDF2788129.1"
                     /translation="MKQKWIVLIIICIGVFMSTLDGSILNIANPTIAADFKINMSQIQ
                     WVVTAYMLVVTATMLFFGKLGDKVGSNRLYTLGFFIFTIGSFLCSMSNNLSTLISSRI
                     FQAVGASILMATGLGIVSNAFPANEKGKAIGITGAVVGIGNMSGPVIGGIILEHFGWP
                     SIFIINIPIGIIAVFLGIKFLPKPVLDEQNKSFDIPGLLLFASCTTLILLAMNEKGNT
                     RLYLGITALIIFLLLALREVKFEQSFIDLPLFKNRNFTVGNIIGVACYFPQMAVSFLL
                     PFYLEQLKNLSPMMAGYVMTVHPLIMVLIAPIAGSLSDKHGAKNILTASFSFMTISLV
                     GMALLKADSPLYLLIVCLVIFGLGLGAFSSPNNSSILADVPPQKQGYGGSFLATIRNL
                     SFALGTAFFSSFFAQSLTYNQKFKSHTSAYVIASNQSYWIAASVCFIGLILTVFFMRK
                     TDKSIS"
     regulatory      complement(24728..24843)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            25131..26210
                     /locus_tag="PC471_000991"
     CDS             25131..26210
                     /locus_tag="PC471_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDF2788130.1"
                     /translation="MEGKVKKAFPGGNTSQGSYSFFDYIIPENVKRVFCLKGGPGVGK
                     SSLMKKIYKEFLHRGYDIDLYHCPSDPSSLDGLVIKKLGVVLMDATAPHTMDPKLPGA
                     LDEIINLGDYWNTEELEKNKQEISVYDKDISNSFKRAFKFLKSAEPIFRDIEEKYSDC
                     MDYGKVNLVTEEFIKRLFDGVKSTGNLKREKHLFGSAITPVGCLDYTENILKDVKKVY
                     YLDGEIGTGKTTLLNKVYKKAIEKGLNVEVYHYPLIPEKIETVLLTDLDIGITISTTF
                     KEEDTIDLNQFLNREKLLKYEEDIKFDEKVLDDLICWAVLNLKRAKSKHDIIESYYSP
                     NMRFDEVGKLRDKLIKRILKYEENL"
     gene            26601..27281
                     /locus_tag="PC471_000992"
     CDS             26601..27281
                     /locus_tag="PC471_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF881 domain-containing protein"
                     /protein_id="HDF2788131.1"
                     /translation="MKKQYVFIILASTILGILVGTIFRDYSNEETVYTSKDKSIRKEI
                     KMTRKSIKELTKEKDKLDKEINSMKQNQKNSEEISEIETLRDSLSYSDVKGSGIILSI
                     DAIDEKTGNIANSIDYNKILVKVINELKVNGGKYIEINGQRINQYSEVVLAGSHININ
                     GVPVAQPYEIKCIGDKEKLSNYVDKGSKYLDSIQTNYPMKISIKTEENISMKKMNIPN
                     KLKYIKGD"
     gene            27274..28653
                     /locus_tag="PC471_000993"
     CDS             27274..28653
                     /locus_tag="PC471_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II/IV secretion system protein"
                     /protein_id="HDF2788132.1"
                     /translation="MIKIILEESSLPMLIPEKLAKKYNVFPVEFQDDNLVVEIEDEDI
                     YALQDLRLATGKEIILKKEKKELISEKIEKYYNSFELDEDYAKKLFENLLEKAIKENA
                     SDIHIEPFKEHLIVRMRVDGELKEVSKFLVDVYPSLSTVIKLKASMDITEKRLPQDGR
                     VDIKLGGNLIDIRVSSIPTVYGEKIVLRILNRDTFLKDKLELGFSEEAIKSINRIINK
                     RAGILLVTGPTGSGKTTTVYSLLNDLKGINKNIMTIENPVEYKMDGISQIQVNSKVGL
                     SFDVGLRAILRQDPDIIMVGEIRDAETAKIAVRAAITGHLVISTLHTNDAVSSIARLL
                     EMQIPPYLLNASLIGVISQKLVRKVCNHCSHEIMIKDNFSGDVNTKVAVGCEKCNDKG
                     YFGRTAIYEILEINDDIKTCIRNMEDSSTIKDVAQNNGMITFEDSCKRLINEKITTLE
                     ECLVVNEMI"
     gene            28824..29909
                     /locus_tag="PC471_000994"
     CDS             28824..29909
                     /locus_tag="PC471_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HDF2788133.1"
                     /translation="MRLSKNIFYNLEKFYLVLNRISDKEIRVICKEIGILLESGCEIT
                     KIFEIIESQSSKKVKNLLSIVSNHIQKGNSIAESFQITGIFSKFFISMIKAGETSGNL
                     DIIMSDLSNYYDKEYKLKMKIITISIYPIILIILSILSMLFIFVFVIPNFQVVFTNNG
                     IEPPLITRVLMGISTVVTNNLAYIIFSFILFSIGSIYFFITNDNIKKLINSLKFKIPF
                     IKKINQLVATTRFSRALSVLISSGIQIVEAIDMSASVIDNEFIYERLKISNNYIKKGN
                     SIGKSLNLANIFPKLFISMANTGEESGKLDKSLNVINKFYENELNNKIEQIMKFIEPM
                     LIVFMGLIIGTFILAMVVPMFDAITNF"
     gene            29963..30322
                     /locus_tag="PC471_000995"
     CDS             29963..30322
                     /locus_tag="PC471_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HDF2788134.1"
                     /translation="MINLINKKRKGFTLVEMIVVVTILGVISSIALVKYSKVQESAKL
                     NADYTNAANIVTAASMAINDDEKTIDSLSVETLKEKGYLNTVPVPQSTSGKFELVIND
                     SGTDISVNINSKQFYPK"
     gene            30375..31247
                     /locus_tag="PC471_000996"
     CDS             30375..31247
                     /locus_tag="PC471_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilM"
                     /protein_id="HDF2788135.1"
                     /translation="MAIIKKILNLEINDDYIRLVFLRKSHKKVFIDKSIIKEINFDIK
                     NDSNSIEKIKYIEDVVRFVKEEIGKSKILYKNLYFNIQIQDIVIRNVEVLNTKKKRDI
                     LSMIEFEITQYIPININNYSIKYKVINSKNDKLNIQVILFPKNIIQLIKNISENLNMN
                     PKTININFDILQKLINLNLIENFSEEGVFIECKKSQLIINITKDKKIRETYLLSKEGK
                     SYEFIKRLLRGFKYVYYYGIENCFIEEYFKYTDKSIEVNPLRLKDKVKVENNFDADLE
                     DNRINYINSIGMII"
     gene            31248..31691
                     /locus_tag="PC471_000997"
     CDS             31248..31691
                     /locus_tag="PC471_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2788136.1"
                     /translation="MKVDKTINFLNGFKKTKPCNKIYKIIIVLILTCILFKSFMYVKS
                     INELEDYISQNKLVDKSKNTLPTTDKTREKFSKIKLNEIKKIYETIGINSITKISIDK
                     NKLEMEGVCSDLDVLNNLRKIKYIKYYYIDSIVRDGEYYRFRIKS"
     gene            31706..32290
                     /locus_tag="PC471_000998"
     CDS             31706..32290
                     /locus_tag="PC471_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2788137.1"
                     /translation="MRLNNREKNLLKILLILVIILASDIAVSYLEENLTSLKNEKQNI
                     INKINNQDNYQIKDNKHIDDKYIESVELNKNEELKNNFNNSFLDKKTNKDIVLLVENE
                     LRDIVNVEYINQESSLDNHNKKQVNLEIKVNGELANVLKLEGVMKKLRLNNKLEKIEI
                     NKLKVNNFDVQNIPNEYKQEKDNIVECKMTVRVI"
     gene            32543..33580
                     /locus_tag="PC471_000999"
     CDS             32543..33580
                     /locus_tag="PC471_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEC-C domain-containing protein"
                     /protein_id="HDF2788138.1"
                     /translation="MLGRNELCPCGSGKKYKKCCLNKDIVSERACRKTELSQKQYTDL
                     YSKLYDYSRQDKFKEECEKAKEMFYIMQNDSVNEKFERFFNTYLIQDHIMENKKVMTV
                     GFLEENGANLSQSEVSILKSMFESYVSIYEVKEVSAGKIVLKDCLNGNELCTEDVKLL
                     RSFKIGSCMIARIVDIEGTNILIDITISISNEVKDIILKDIMNLFNQYQDVYKDMKSF
                     LIYHTHILYKYIQQLLDPSIAEYLKRERDKNDTKVNSENELAEEECKVLDTLKENVEK
                     ENIDACIEFWNEYKKNHENIKGAENGWAAAVEYYVKKEAGQPITQVQISKKYDISSST
                     LGKRYKDLKIS"
     gene            33661..34026
                     /locus_tag="PC471_001000"
     CDS             33661..34026
                     /locus_tag="PC471_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2788139.1"
                     /translation="MSLSLGNAIAVSTNFIKSNPAVPVYNGEEIYAVIYHIEIGYNNG
                     VSPDTKQYIIIDYKEFDLSMDKKNELINIAKEYCKSKGIVENENLEINLLDNEEAENF
                     LEKVFNNMKVIRGLITKKG"
     gene            34031..34348
                     /locus_tag="PC471_001001"
     CDS             34031..34348
                     /locus_tag="PC471_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2788140.1"
                     /translation="MDYIGIENITPYENTYEFSVYEYDDEITLGSEKLYVCELRVVLI
                     KVNSLYVERLHKSVEAMVLVKNLKKDLDKTLVVNKIKNFVLDEIWVENLVKENIEVIF
                     VES"
     gene            34396..36000
                     /locus_tag="PC471_001002"
     CDS             34396..36000
                     /locus_tag="PC471_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2788141.1"
                     /translation="MLRVSNVKLGIDKDISLLKDVILKKLKIKEKNLVKYSIYKESID
                     ARKKGKMEFVYSVDVEVKDENKILNSKIKDVTKIKEIRYVNVPVGDKKLKNNPLVIGS
                     GPAGLFAGLLLAQMGYEPIILERGLDVDSRTKDISDFWTTRKLKNNSNVQFGEGGAGT
                     FSDGKLTTRIKDIRCRKVLEELVQFGAPDEILYSHKPHVGTDILKNVVKNIRNEIISL
                     GGQVRFNSKVTDIVVSNDSIQSVIINDTEKIDTDTIILAVGHSARDTYEMLYSRGIKI
                     VQKPFAIGARVEHPQDLINKAQYKDFYNHPRLGAADYRLIEHVSNGRTAYTFCMCPGG
                     SVIASASNDFEIVTNGMSEHARDKINANSAFLVNVTPEDFKSDNPLAGVHFQQKYEKL
                     AFELGGKNYNAPVQLVGDFINNKTSLNLGSVEPSYRPGYTLTNLSECLPSFVTETMRE
                     AFISLDKKLNGFAMYDAVLTGVETRSSSPIRIVRDENTLESVSIKNLYPCGEGAGYAG
                     GIVTAAVDGIKCAEKIIQKYTNNTKI"
     gene            36124..37752
                     /locus_tag="PC471_001003"
     CDS             36124..37752
                     /locus_tag="PC471_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HDF2788142.1"
                     /translation="MNIIISLMGGLGLFLYGMNLMGEGLQKSAGTKLKKIIKLLTSNL
                     FMGVLVGTGVTAVIQSSSATTVMVVGFVNAGIMTLKQAIGVIMGANIGTTVTAQLVSF
                     DLTGMAPVALGVGIILYLFGNKPRIKNIAEILIGFGILFTGMDFMKMAVEPLRDYQGF
                     TDLLVTFGRYPLLGLLLGFGITAIIQSSSASMGMLVALAAEGLVPLSAALPILYGQNI
                     GTCVTSLLSSIGANKNARRAAMMHLIFNVLGTVIFLIFLNKPVVSMVTSWDPSNVARQ
                     IANTHTLFNIISVLILLPFTNLIIKLAIKLVPDRAGDIDEDETKTIKYIDDRMIETPS
                     IALANTIKEALRMGEKAKESLNASMEALVEHSTEKIDKTYRRERLINDLQKAILNYLL
                     KLSKAPLDDDSREVVDTLFNTVNDIERIGDHAKNIAELSQVAIDSNISFSEEGQSELD
                     VMYNRVVSAYTYALESMRTDNVDLACKVIKIEEQVDIMEKSCRANHMYRLNNNLCSIE
                     NGVIYLDVISNLERISDHAVNIAQQVIAKRLGND"
     gene            38017..39366
                     /locus_tag="PC471_001004"
     CDS             38017..39366
                     /locus_tag="PC471_001004"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HDF2788143.1"
                     /translation="MGKINFDYSKATEFFCQNEIDVMQPYVDVAHDMLHNKTGLGNTF
                     LGWIDLPKNYDKEEFDRIKKSADKIKSDSDVLLVIGIGGSYLGSRAAIDMVSHSFRNG
                     LKKEQRKAPEVYFVGHNISSTYIMDLLDIIEGKDISVNVISKSGTTTEPALAFRIFKD
                     YLEKKYGKEEARKRIYATTDASKGALRQLATEEGYETFVIPDDVGGRFSVLTAVGLLP
                     IAAAGLDIDAMMKGANDAREAFQNPDLKSNDSYRYAVARTILHRKGKDVELLVNYEPQ
                     LHYVSEWWKQLYGESEGKENKGLFPASVDFSTDLHSMGQYIQDGKRLLFETVLNVENC
                     KRNITISSEEVDLDGLNYLAGKTVDFVNHKAFEGTLLAHTDGKVPNLVINIPQLDEYN
                     FGYLVYFFEKACGISGYLLGVNPFDQPGVEAYKKNMFALLGKPGYEKEKEELEKRLK"
     gene            39522..40601
                     /locus_tag="PC471_001005"
     CDS             39522..40601
                     /locus_tag="PC471_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HDF2788144.1"
                     /translation="MSRRKKGISLVILVAIISSILSSFLTIILVKDNLVSKSTGSSTP
                     IVVNDDGKSQNIYQAVAEKATPSVVGITTTSVDTSNMFAIPTETQGVGTGIIVDSNGY
                     ILTNSHVISDGQATSVNVLFNDGSTTSGKVVWFDQQLDLAIVKVDKTGLTPAEFADSD
                     KVKVGDISIAIGNPLGLDFQKTVTQGIISGLDRTIQTEKTNMTGLLQTDASINAGNSG
                     GPLLNQKGQVIGINTAKASQAEGLGFAIPINTAKSIVEEVIKNGKYEKVTLGIKGTDV
                     SNYEAATGTKLSTDKGVYVAEVISGSSAEKAGVKVGDIITKVGDTDITGMNDLNKKLY
                     TFSKGASTKITVNRGGKAVTINVNF"
     gene            40946..41854
                     /locus_tag="PC471_001006"
     CDS             40946..41854
                     /locus_tag="PC471_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HDF2788145.1"
                     /translation="MNPLVINLQKCSIHDGPGIRSTVFFKGCPLECVWCHNPESQTYT
                     KQVLYNEERCSKCEACINICPHKAIYKGETKICLDQDKCEFCETCLDYCVNNAREIVG
                     QEYSVRDLVKEIEKDRIFYEESGGGVTLSGGEVMAQDMDFICGVINMCKSKGIHVAID
                     TCGYAKSENYERVAKCADLFLYDIKLIDEDKHIKFTGKSNDLILKNVKILSELGANIN
                     IRIPLIVGVNVDDENLEVKKMIEFLKPLNIQAVSLLPYHNIGKHKYDKIYKKYEGEEL
                     QRPSEEKLEEIKRLFEASNFNTKIGG"
     gene            41859..44228
                     /locus_tag="PC471_001007"
     CDS             41859..44228
                     /locus_tag="PC471_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HDF2788146.1"
                     /translation="MARGTFERTKKLREESINAEPHISIERAVLMTEAYKKYEGSVEI
                     PVLRALSFKHYIENRTLSINDGELIVGEKGDSPNGAPTYPEICCHTMEDLEVMHNRDI
                     INFSVSEEARKIHKEEIIPFWKKRQTRDKIINAMTPEWLAAYEAGMFTEFMEQRAPGH
                     TVCGDTIYKKGFLDLKKDIEARLKELDFLNDLDAYNKKADLEAMAIACDAMVILGKRY
                     AEKARQMAEEETDEAKKKDLLLIAETCDVVPAHKPETYHQAIQMYWFVHIGVTTELNI
                     WDAFTPGRLDQHLNPFYERDVENGILDRDRAQELLECLWVKFNNQPAPPKVGITLKES
                     STYTDFANINTGGINPDGQDGVNEVSYIILDVMDEMKLIQPSSNVQISKKTPQKFLKR
                     ACEISRKGWGQPAFYNTEAIVQELMEAGKTIEDARLGGTSGCVETGCFGKEAYVLTGY
                     MNIPKILELTLNNGYDPISKKQIGIETGDPRNFQSYEELFEAFKKQLHYMIDIKIEGN
                     AVIENICAKHMPCPLMSTIVDDCIEKGKDYQRGGARYNTRYIQGVGIGTITDSLTAIK
                     YNVFDKKKFDMDTLLKALDANFEGYEAILNLVSNKTPKYGNDDDYADEIMQEIFNAYY
                     NEVTGRPTVCGGEYRVDMLPTTCHIYFGEIMGASPNGRLCAKPVSEGISPEKGGDTNG
                     PTAVIKSCAKMDHIKTGGTLLNQRFAPSVVQGEKGLDNMANLVRAYFNMDGHHIQFNV
                     FDKNVLLEAQKNPQDYKDLIVRVAGYSDHFNNLSRTLQDEIIGRTEQTF"
     gene            44553..45356
                     /gene="proC"
                     /locus_tag="PC471_001008"
     CDS             44553..45356
                     /gene="proC"
                     /locus_tag="PC471_001008"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HDF2788147.1"
                     /translation="MKTLGFIGSGNMGSAMIGGIVQSGLVKAEDITVSDLSQVALDKV
                     KEKFGVNTTTDSKVAVTNSDVVIVALKPNICEKALTPLKELIDANKIIVSIAAGKTIE
                     SLESFIGSDKKIVRVMPNTPALVGEGMSALCKNSNVTDEELNMIKALFESFGEAEIVS
                     EYLMDTVTGISGSSPAYVFMFIEAMADAAVLAGMPRQQAYKFASQAVMGSAKMVLETG
                     KHPGELKDMVCSPAGTTIEAVKVLEEEGFRAAVIKSIVACIDKSKDMSK"
BASE COUNT        18670 a         4819 c         8244 g        13758 t
ORIGIN      
        1 atggttaatg tgaattttaa agatattatt aaatatataa attagaatac tattaaacgg
       61 ataaatggag gagttattat gtctaagata ttagtagtag atgatgagtt agatatgtta
      121 aagttgattg aaaatgtgtt gaaaagagat ggacatgaag taaaggtaat tagtgatgta
      181 gaaaaagcta tgaacatgga ttttaattat tttaatctaa taatattaga tgttatgatg
      241 cctaagatgg atggttttga agtatgtaaa actataagaa ataaggttga ttgtccaata
      301 ctgtttttga cagcaaaaaa tatggaaagt gatattatgt acggtcttgg tataggtgca
      361 gatgactata taaccaaacc ttttggtgta ggagaattaa gggctaggat aactgctcat
      421 ttaaggcgag aaagtagaga aaaaaagaat ttattaacta tctcaaatgt tcaatttaac
      481 tttttaggca aagaagtttc aattgatggt aaaaaaatta attttactaa aagtgaatat
      541 ctaatatgtg aatttctagc aaaaaataag ggacaagtat tttcaaaaga aagaatatat
      601 gagtcggtat atggttttga tggagaaagt gatataagta ctatcacaga gcatataaaa
      661 aatatacgaa gcaaattaaa acaatatgat atttatacaa tagaaactgt atgggggata
      721 gggtataaat gggtgtaata agtataagtg ttgtttagtg aaaatattat atatatctac
      781 aaaatgtttg taaaatatta atctatatat taaaatttaa tttatagatt aatatttttt
      841 gtaaatgaca aaattgtaag gtattttgtt ataaaatatt aagattcata gtgtatacta
      901 ataatatagt atttagtata aatcttgagc aaaggatggt tatttctaat ggatagacta
      961 aagaatatta gaaagagttt actacagtat acatttttat ttttgttaat aggtataaca
     1021 atttacctag tgtttaaaac attggatatt aaaatgctat caaatgttat ggttatggta
     1081 gataagaagt ttttattcat aggtgctttg gcaataatgt gtcacataat gcttgaagga
     1141 gtagtaatga agataattat agaaagcact cacaaagtta atattagatt tattggtttt
     1201 aagctagcca ttatgggatt ttattataat ttaataactc cttttgcttc aggaagccaa
     1261 ccagtacaga tatatgtact taaaaaatgt aaaatgccac ttagtaaggc aagtgcagtt
     1321 gttaccaata agtctattat cttccaaata gtagttactt tttattgtac aatacttgtt
     1381 ttaatgaatt ttgctatgtt agaaaaacaa atgaaaatga ttatgcctct agtgtttcta
     1441 gggatagcca ttaattcatt tgtcttaatt atgataatat tagttatttt aaatcctgaa
     1501 aaaataaaat attttatgag aattactata aagcatttat caaaaattaa gtttttaaaa
     1561 tttttagata ataagattga atccatagaa agttttatag atgattatag taaagcaata
     1621 aatttttttg caaagaataa aaaggtatta atttcaacta taatagtaac ttttattcaa
     1681 ttaagtgctt actttagtgt ttcattttgg atatataaag catttaattt acaaggttat
     1741 agctatattt atatactgac attacaagca tttttatata tggcaatatc tccaatacca
     1801 acacctggga atgtaggagc taatgaatta gcctttttca ctatattcaa atcagtattc
     1861 cctcaacctt tgatgggata tgcagtattt ttatatggtg gatttatgta ctatttgatt
     1921 ctgattggaa gtggaatatt tactgtaatt actcactata gaatgaaaaa cagaattggc
     1981 agaaatattg ttttaagtga aaactaaata agtttaaaga atatatattg agtatattaa
     2041 aaaatatgtc ataagaacta gaatatttta attaaatggt tgaaatataa agattattat
     2101 gacatatttt ttttgaaatt taagaggaat tgtgagaaaa ttgttgaatt taatagatag
     2161 tattgcaaaa tatttatgat tttgcatatt ataaaaacac tcattatctt taagataaat
     2221 tttacttatc aggggataaa tctaaggggg gaattgtatg gagaaaaaaa ttttaacagt
     2281 ttgtccatac tgtggagctg gatgccaatt atatctagta gtaaaagata ataagatatt
     2341 aagagctgaa ccggctaatg gtagaacaaa tcaagaaagt ttgtgtttaa aaggtcgtta
     2401 tggatgggat tttctaaatg accctaaaat tctaacacct aggctaaaaa agccaatgat
     2461 aagaaaacaa ggtgtactag aagaggttga atgggatgag gctataaact atacagcttc
     2521 aagactaaat gaaattaaag aaaaatatgg acctgattct attatgggaa caggttctgc
     2581 aagaggacct ggtaatgaag caaactatat aatgcaaaaa tttatgagag ctacaatagg
     2641 aactaataat gttgaccatt gtgctcgtgt ctgacatgca ccatctgtag ccggtctggc
     2701 ttactcatta ggtagtggtg caatgtctaa ttctatacca gagatagaaa atgcagatgt
     2761 tttatttata tttggatata atggagcaga ttcacatcca atagtggcaa atagaatagt
     2821 aaaagctaaa aaaaatggtg ctaaattaat tgttactgac cctagagtaa ctgaatctgc
     2881 aagaattgct gatatacatc tacctataaa aggtggaaca aacatggttt tagtaaatgc
     2941 ttttggaaat gtattaatag aagaaggtct ttacaataaa gaatttgttc aaaatcatac
     3001 acaaggattt gatgagtata aagaaattgt taaaccatat acagctaaat atgctgaaaa
     3061 gattacagga atcccagaag agcttataag aaaagctatg agagaatatg caaaaggtaa
     3121 aaaagctatg atactttatg gaatgggtgt ctgtcaattt ggtcaagctg ttgatgtagt
     3181 taagggactt gcatcaattg ctctacttac aggtaatttt ggtagagaaa gcgttggaat
     3241 aggacctgta cgtggacaaa ataatgttca aggagcttgt gatatgggag ctcttcctaa
     3301 tgtatatcct ggctatcaaa atgtaactga tgataagata agagaaaagt ttgaaaaagc
     3361 ttggggggga aaattatctc ctaataatgg ttatagttta actcaagttc caaatttagt
     3421 actaaaagag aaaaagctta aagcttatta tatatttgga gaagacccag tgcaaagtga
     3481 cccagatgca tcagaagtta gagaagcatt agatgaattg gaatttgtca ttgttcaaga
     3541 tatatttatg aataagacag cacttcatgc tgatgttata cttccagcta cttcttgggg
     3601 agaacatgag ggagtatata catgtgcaga tagaggattc caattgatga gaaaagctat
     3661 agagccacaa ggagatgtaa aaccagactg gcaaattata agcgaaattt ctacagcaat
     3721 gggatatcct atgaattata aaaatactaa ggaaatatgg gacgaactta gacaattatg
     3781 tccaagtttc ttaggtgcta cttatgaaaa gatagaaact caaggatgcg ttcaatggcc
     3841 ttgtaagagt gaaagtatgg aagataaagg aactatgtat ctgtatgaag gtcaaaagtt
     3901 ttctactcct aatggtaaag gtaatttgtt cgctgctgaa tggagacccc ctatggaagt
     3961 tgaagatgat gaatacccat ttagtttatg tactgtaaga gaagttggac actattcagt
     4021 aagaacaatg actggtaatt gtagaacact tagttcatta gaagatgaac caggacgtgt
     4081 tcaaataaat tctaatgatg ctgaaaaatt aggaattgaa gatgatgaat tagttagaat
     4141 tagttcaaga agaggaagtg taataacaag agcaacagtt actgatagag ttaaggaagg
     4201 tgcaacatat atgacttatc agtggtgggt tggagcttgc aatgaactta caatagcaaa
     4261 tttagaccct atatctaaaa caccagagta taaatattgt gctgttaaac ttgaaaaact
     4321 tgaagaccaa gaattagcag aaaaatgtgt aagagaagag tatcaaagtt tgaaagacaa
     4381 aatgacagct acaaatatct aatgatttat tagatttaag atttataaaa ggcagaaagg
     4441 ctaatatttt atgaacaata tatactcaaa aactaaatta caagaaaata gtaaggataa
     4501 agtttttgaa aatagttata gtctagtgga ctacaaatat ttaaattctt ttaatgtaga
     4561 agtaagcaaa ataaatgaac atgaagtatc aatagataat gaagaaataa ttgcagaata
     4621 tccacttagt tttattttaa atgataaata tgtaaataca tttttatgta ccccatataa
     4681 tttagaggaa cttatagtag gattcttgaa aacaaaagga tacatagaaa acaaaaaaag
     4741 cttattaaaa tttgagataa atgagagtga acgttttgca aaagtaacta ttcaagagaa
     4801 acaaaaaaat caagatgagc aaattatatt tttaaattca ctagactata taacatgtgc
     4861 tcctgtaaag agcaatataa aaataaatta tgatacgatt tacagtataa tggataaaaa
     4921 tttaaattca tcagaattat ttaaaaatac tggtggggta catagcgtat ctgtatttaa
     4981 ttttgataaa aaaatagtca cttgtgaaga tgtagcaagg cataatgcaa tggataaggc
     5041 tattggattt tgtgttttaa atgacataac attaaaagat aaaattgtgg ctgtaagtgg
     5101 aagaatatca ttggaaatga tattaaaagc agctatgact caaattccta ttgtagtatc
     5161 aaaatcagca ccgactaatt tatcaataga gctttctaaa aaattaaata taacgcttat
     5221 tggatttgta agaggaagac ggatgaatat ttatgcaaat ccacagagag ttatcaaaaa
     5281 gtgagaatga atatatgtgt actaaaaaag aagagcaaag atattcaaag tgtgaaatct
     5341 gtggagtatt aattgatgat actgataagg tgtataataa cacatacaat ttgtgcaaaa
     5401 aatgtaaacg aaatataagt actgagaaaa tagtgaaata tataaatata tatgatgaaa
     5461 gtaagtagac atatgatgaa aataactaga gggttggaaa tatacaaatt gaaaggtggg
     5521 ataatatgaa aaataaaata ggctcttttg taatagcaga cccaaacaaa tgtacaggtt
     5581 gtagagcttg tgaagtagct tgttttacaa tgcataatca gcataataat gtgggttata
     5641 cagttggaac agttgatata cctgttatac ctagacttta cttagtaaaa ggtgacaatt
     5701 tctgtatgcc aatacagtgt agacattgtg aagatgctcc ttgtttaaac tcatgtccac
     5761 aaaaagctat agttaaagaa aacaatatta tgtctgtaaa tgaagaaaag tgtataggat
     5821 gtaaaacttg tctgctagca tgtccttttg gagctataga cttacttcca caatatgaag
     5881 atggtaaaga agtagaacaa gtaattttag atgaaaataa gaagatagct tataaatgtg
     5941 acttatgtaa agacaatgaa aaaatagctt gtataagtgc atgtcctcag gaagcattaa
     6001 aacttgttac tccaatagat gacaaaaaag ctaagaatag acaagctgca cttagtttat
     6061 tgttaacaaa taaataaagg atacaggggt aggtgacgtt tatgataata aatatagata
     6121 aggatttatg tactggatgt agagaatgtt caaaggtctg tccagtaaat gctattgaag
     6181 gtgaagaggg aaagccacaa gagataaatt tagatagatg tgttatgtgc ggtcaatgtg
     6241 ttcaaacttg taaatcttat gcctcagtta tagatgaagg atttgagttt ttacaagaga
     6301 aaaaacaaga aagagagata ccagagtcta taaatgaacc tatatttgca gcatacaatg
     6361 tatgtgatat agataaggtg aagaaagctt taagtgaccc aaatgtattc actatggtcc
     6421 aatgtgcacc agcagttaga gttgctttag gagaagattt tggatattat ttaggatact
     6481 taggtgctgg gaaaatggca gcagcactta gagctttaaa ttttgataga gtctacgata
     6541 ctaattttgc agcagattta actataatgg aagaaggtag tgaattaatt aaaagagtga
     6601 cagaaggtgg aactcttcca atgtttacat cttgttgtcc agcttgggta aaattcatgg
     6661 aaaaaaatta tcctaaatta actaatcatt tatcttcttg taaatcacct cagcagatgg
     6721 gtggtgcaat atttaaaact tatggtgcag agataaataa tgtagatgca tcaaagatat
     6781 ttagtgtttc tataatgcca tgtacatgta aaaaatatga atgtgataga gaagaaatgg
     6841 attctagtgg atatagagat gtagatgtag tattaactac tagagaacta gcttatttaa
     6901 taaaagacat gggaattgat tttaaaaatt taagagaaga aaaatttgat agtcctttag
     6961 gtagttatag tggagctgga actatctttg gtgttactgg tggggttatg gaagctgcta
     7021 taagaacagg atatgagctt ataactggag aaagtattcc tgatgtagaa gtaaaacaag
     7081 taagaggaga aaatggtttt agaaagtcaa ctattaaaat aggagactta gacttaagag
     7141 ttggagtagt aagtggatta aaaaatgtaa taccagttct tgaagacttg gaaaaaggaa
     7201 aattagatgt agattttatt gaagttatga cttgtcctgt tgggtgtgta agtggtggag
     7261 gtcaaccaaa aattttacta gaagaatata aggaattggc ttatgaaaat agaacaaaag
     7321 caacatacgt gcatgatgaa catttagcaa ttagaaaatc tcatgagaat cctgatatta
     7381 aaaaactgta tgatgaatat ctggtagaac cattaggaga aaaatctcat catctattac
     7441 acactacata ttgtatagga aaggaagtgg ctaagtgatg aactattttg ttatagcaga
     7501 ccctgataaa tgcataggat gtagaacttg tatgatagga tgtgtagttg cacattcaga
     7561 tgaagatatt ttttaccaaa atccagatga aataaatttt aatccaaaac tttctgtcat
     7621 aaagacaaaa gaagtaagtg ctccaataca gtgtagacat tgtgaagatg ctccttgtgc
     7681 aaaggcatgt ccaaatggtg gtatagttag ggttggtaac acaataaaaa taaatgaaga
     7741 aaattgtata ggatgtaaga cttgtatgtt agcatgtcct ataggtgcaa tagacatagt
     7801 tacattaaaa gatgtagatg aaggaaagct ttgctttaga gaaagaatga cagcaaataa
     7861 gtgtgatttt tgtatagaat cgccagaagg gcctgcatgt gtaaatgttt gtccaactaa
     7921 ggcatttact atagttaagg atgaggatat agatagtaaa gttaaaaata agagaaaatt
     7981 agctatattg taatataaaa aagatggagt tttatttatg attaaaagct ccatcttttt
     8041 tattataata tttatatatc taaaaatcaa ttattgcatt agtttatcta atctttcttt
     8101 attcaatttt ttcattccta tagaagttat aataagtaaa gtataggcaa ttataactga
     8161 taaaattata atagaccaag aaaatctgta tccaaaatta tcaactaaca atccaaataa
     8221 tgttgaacct aatgcaaatc ccactgcaaa aattaattgt acgattccat atatgctacc
     8281 aaactccttt tgaccaaaga aagagccagt taaataagca ggaccaatca tataagcaaa
     8341 tacagaaaga cctttcaaaa tggcaaatac aaatgcaaaa gtagaagatt tatctaccaa
     8401 tagtaatgat aaagttgcta taagaaccaa tacaaaagat ataataagtg gctttgtttg
     8461 accgaattta tcaaacaata atcctccaaa taaattacca atgagagcaa ataaggcaaa
     8521 tgtggagcct actgttccta ttagaatagg atttagataa tttctaagat aagttggata
     8581 ttgtacagat aatgatgaaa catatatacc aacaaatata aatccaatag tcataatcca
     8641 aaaatattta atttttttag cttcttttaa tgtataaccc catactgaag gagaaattgt
     8701 atcagatgct ttctcttttt tacctttaga ttttaccttt tcattaatat ctttaggcat
     8761 cctgagtatg aataatatta caggtatacc aactattaat gctataattc caaatattag
     8821 ataagaacga ctgtatcctt gagttgctaa taagcgaact gcaagtggtt gtagaaatat
     8881 actaccaata gaaccaccag caaaagcaaa gcccaaagct tttcctttgc tttcttcatc
     8941 aaaccaagca cttattaaaa caggtattcc tatagcagat ataattgtag cgccaatttg
     9001 aacaatacat gatacagtgt aaaattgcca tagttcagta catatagaaa aagtagcaaa
     9061 agcaatacta gaaattatac aacctaataa atatagtatc ttaatattga ctttagaaaa
     9121 taatacacct ataaaaggtg atgcaattgc tgaggcaata gtaccaatag tgaatattag
     9181 agaaaaagaa gctaaactaa agccttctcc ttctacaaca taacttataa attgaggctg
     9241 tatacttgct acaatactaa atggaacagc ttgtattaac atacaaccaa gtactataac
     9301 ccaagcattt gaaaagtttt taatgctaaa tttattgttt agtaatgtat tcatggtatc
     9361 tctcctttag gttatgattt gtagatatta atctaattat tctaatttta tatattagtt
     9421 ttggatagcc taactattta aatataattt gggtattaat ataaaaatta actataaaaa
     9481 ttgattattt taaatgtatg gggtaaatta aagtatataa ataaatgaaa ggatggataa
     9541 aatgtcattt gaaaaaataa ataataataa cctagataaa atacacaata ggaagtgtat
     9601 tatgcttgta aactttaata aaaaagaatt atcattaatt aaaaatgtaa gtaactttgc
     9661 tggaataaga gaccatatca tcttaagtga taaaaatgga gagaatataa taagagacat
     9721 tttagatgga aatatatcaa atgaatgtga aaatgggatt aagagtaagt ctgtaatatt
     9781 taataacact ccaagtacac aagtaaatgc atttttagaa gggctaaaaa aagtgagaat
     9841 aaatagacct tttacagcta tggtaacaga gacatctatt gattggacat tgaataattt
     9901 aatagaaaat ttagttcaag aacgtaatgc tatgtcttta ggaaaaacaa ttgaacataa
     9961 gtaaatttaa gatatcttat agtaatttta actaatgtac attgagtatc atattatatt
    10021 ttagaataga aaattttaaa tgtgggtaga tggaggagat ttgagatgaa agttttattt
    10081 acaataaatt atggaaaaga aaaatttgat aaaataagag aattaggcta tgatgtcata
    10141 cattactctg aagatagtat tgaaaataat gaggatgtaa atacagcaga tgtactagtt
    10201 acatacaatc catttgagag attagatatt tctaagatga aaaatttaca atatatacag
    10261 acaactagtg taggtataga ccaacttcca aaagaagagg tattgaagag aaatattaag
    10321 atagctaata atagaggcgg atatagtgta cctattggtg aaagtattgt attgtatatt
    10381 ttagaaatat ataaaaatag tgcaaaattt tttaaacaac aacaaaataa agaatggaag
    10441 atggattttt ctgtatcaga attgtatgga caaagagttg gatttttagg aacaggtact
    10501 atatcttcag aagcagcaaa aagattaaaa gcttttggtg tagaaatatg gggtgtcaat
    10561 actgatggaa gtagtaaaaa atactttgat aagtgttttg ctactgagga tatgaatatt
    10621 gtatttgaag aatgtgatat agtagttgta gcaatgcctt ctacaaaaag tacagatgga
    10681 atagtaaaca aagatatgtt taaacttatg aaagatggtt ctgtatttat aaatgtaggt
    10741 agaggaaata cagttaatca gaaagattta gaagaatgtg tagagaaatt tagaggggta
    10801 gcattagacg tatttgaaag tgaaccttta aacaaggaca ataagttgtg ggagtgcgaa
    10861 aatgtcatag taactcctca taattcttgg gtgtcagata aaaacaaaga gagaacattt
    10921 aatatggttt ataataattt gaaacactat ataatggaga ataaacctgt agataatgtt
    10981 gtagacatat caagaggcta ttaaaaataa aaaattttga taaaaataaa aactcttatt
    11041 agtcgtatta aagatactaa taagagtttt ttttgtaata aatttaaagc ccaaaaagtg
    11101 atatactaaa aaatatataa ataggagata attaattata gggaaatttc aaaataattt
    11161 aattttaaaa atatgaagag gtgcaaaaag tgagcgtaaa agatgaaatt aataaactta
    11221 tagagacaat aaactaccat aatgaaaggt attataatca agatagccca gaaatatcag
    11281 attatgaata cgataagtta atgaaggagt taatttcttt agaagaagaa catccagaac
    11341 taaagagaat tgattctcca acaaataggg taggaggtaa gccattagat aagttcaatc
    11401 aaattgtaca taaaactcct atgttgagtt tatcaaatgc tttttcagag caagatttga
    11461 gagattttga taagagagtt caagaatatg tgggagaaaa tgttgaatat gttgttgaat
    11521 ttaaaataga tggtctttct gtaggtctaa cttataacaa tggagagttt gaaaaaggag
    11581 caactagagg agatggagtt gtaggagaag atatttctca aaacttaatg acagtaaaaa
    11641 gcatacctct taagataaat gataaaaaag aactaatagt tagaggagaa gtatatatat
    11701 caaaagataa ttttagaaaa gtaaatgaac aacaagaaga acaagaacag ccattatttg
    11761 ctaatccaag gaatcttgca gctggttcat tgagacaatt agacccaaaa ctaacagcaa
    11821 agagaccatt agatatattt gtatttaata tggaaaatat agaagaatac gatttagaaa
    11881 gtcacagtga atcactagag tacttagaaa aactaggatt ttcagtaagt cagagttata
    11941 aagtttgtaa aagtatagat gaagttattg agcatattga atactggaca gataacaggg
    12001 gtaatttaga ctacgaaata gatggtatgg taataaaagt aaacaatata aaacaaagag
    12061 acgaaatggg atatacagcc aaaagtccta gatgggcaat tgcatataaa ttcccagcag
    12121 aaaagaagaa aactaaaata gtagatataa tagtagaagt tgggagaact ggtacaataa
    12181 caccttcagc aattcttgaa ccagtcagac ttgcaggtac cactgtaagt agagctacac
    12241 ttcacaatga agattatata agagaaaaag atataaaaat aaatgataca gtattagttc
    12301 aaaaagcagg ggacataata cctcaagttt tagaagttat aaaagaagaa cgtactggag
    12361 atgaaataga ttttaaaatg ccagaaaagt gtccagtttg ttcagagcca acaataagat
    12421 tagaaggaga agctgctgta aaatgtatca atatgtcttg cccagcccaa ataagaaggg
    12481 gaattataca ctttgtgtca agggatgcaa tgaatataga tggattggga gaatctataa
    12541 ttacactgct attaaatgaa aaaattattc aagatgtatc tgatttatat tatataaaaa
    12601 aggaagatgt agtcgaccta gagagaatgg gcgaaaaatc tgctgacaat ttaataaatt
    12661 caattgaaaa atcaaaagaa aatgatttat ggagacttat aaatggatta ggtattaaat
    12721 ttataggagt taaagcagct aagatacttg catataactt taaggattta gatgaagtaa
    12781 tttctgcaac tagtgagcaa ctagaagaat tagaggaatt tggaagtata atggcaaaca
    12841 gcgttgtcga gttttttaag gaagacaaaa atatgaacgt tataaacaag ttaaaaaatg
    12901 taggagtaaa tacaaaatca ttggataata caggtgaaaa agtagaaaaa atatttgaag
    12961 gtatgaaaat cgtattgact ggtacacttc ctacattaaa aagaaatgat gcaaaagata
    13021 tgatagaaaa aagaggtgga aaagctacat ctagtgttag taaatcgaca acatttgttt
    13081 tggcaggtga agaagctggt tctaaactta ctaaagcaaa tgatttagga attaaagtta
    13141 tagatgaaga gagattttta gaattattaa agttatcatc gaaagatgaa gtaaaagctg
    13201 tattagagag ttaaaaagtc gcttaattta gtagaaaact actataatta atgatagaat
    13261 agttcagtaa gctaataaga ttacgaaaaa tgacaataat taatatttga caaaattaaa
    13321 aattataaaa ataaatttat aaaaaataaa aaatatgaga aaggatataa aaaatgacca
    13381 gaagttatga tagaagtaat gaacagataa gacctgtaaa gataactaga gattatacaa
    13441 agtatgcaga aggttctgtt cttatagaaa tgggagagac aaaggttata tgcactgcat
    13501 cagtggaaga aaaagttcct ccttttttaa gaaatagtgg tactggatgg ataaatgctg
    13561 aatattctat gctaccaaga gcaacacaac aaagaaagat aagagatgca tctaggggaa
    13621 aaattgatgg aagaacacaa gaaatacaga ggttaatagg aagaacaata aggtctgttg
    13681 tagacttgaa taaattagga gaaagaacta tttgggtaga ttgtgatgtg attcaggcag
    13741 atggtggaac tagaactgca tctataacag gggcatttgt agcagttgca gaagcaatat
    13801 ataaattata taaagcaaag acaataaaag agatgccaat aacaaatttt gtgtctgcaa
    13861 ttagtgttgg tattgtaaat ggaatacaca tgctagattt atgttatgaa gaagattcta
    13921 aagcacaggt agatatgaat ataataatga ctgataagtg tgagtttgta gaagtacagg
    13981 ggacaggaga agagaaacct ttttctagag cagatttaaa tttattgtta gagttaggtg
    14041 aaaaagggaa taaagaatta ataaggcttc aaagagaagc tttaggtgaa atatcagatg
    14101 agatattggg tatggattat ggtaatgaag tagtgattgc tactaataat gctcataagc
    14161 tagaagaaat aggtgaaatc ttaaaagatt ttgaatataa agtgtactct ttaaaagatg
    14221 ttgatttagc aggtatagag atagtagaag atggaaaaac atttgagcat aatgcgttga
    14281 taaaagctag aacaatagca aaaaagacta aacttatagc aatatcagat gattctggct
    14341 tagaagtaga tgctttaggc aaaaaacctg gtgtttattc agctagatat gcaggagaac
    14401 atgcaacaga tgaagaaaat agaaaaaaac tgttaaaagc tatgcaaaat gtaccaatga
    14461 gcaaaagaaa tgcaagattc gtctctgcaa tagcagtagt ttttcctgat ggaaaagaat
    14521 ttgtagtaag aggaatttgt gaaggaatga taggatttga agaaaaaggt aaaaatggtt
    14581 ttggatatga tagcttattt atagtaaaag gatatgataa gacttttggt gaaataccta
    14641 gtgttataaa aaattcaatc agtcatagag caaacgcact aaaacttatg aaacaagagt
    14701 ttataaaaag ggtagtgaag taatgaaaat aggcatagta agcgacacac atatgatgat
    14761 taaaaatatg gaaaaaacaa taccatattt gaaagaatgt gacctaataa ttcatgcagg
    14821 agataacttt actgattcaa gatatattca tagcatgact aatgtaggta ttattgcagt
    14881 taaaggtaat tgtgactttg atgctgttga ggaagaagtt gtctttgaag tggcaaataa
    14941 aaccatattc ttatgccatg gagataagta tggtgtaaaa tatggaacta atatgttaga
    15001 aaaaaaggca actgaagttg atgctgatat agttatattt ggccataccc ataccccatt
    15061 tagagaaatt aaagatggtg tactttacat aaatcctgga agcacttcac ttccaagagg
    15121 agtatcgtat aaaagttttg tcattatgga tatagaagaa gatgacataa aaattgaaga
    15181 aatacgcata taaaggtgta tttatctaaa aaaatggcaa aaaacgctca aaaaattaaa
    15241 ttttatcaaa taagtacaac gatattaatg gaacacttgt aaataaaaaa acttatgtga
    15301 tataattaag tggttgaatt ataaatagaa tgaattataa taccgttatc gggtattaca
    15361 taaatatcac taggaggatt agaaggatga aagcagaact aattaaaaaa gagggtaata
    15421 aagttacttt aaaaataaca gtagataaca ataaatttga agcagcagta aataaggctt
    15481 ataataaaag cagaaataaa tacaatatac ctggatttag aaaaggtaaa gctccaagaa
    15541 ttgttataga gactcaatac ggaaaaggta tattctacaa tgatgctata gaaatattat
    15601 tcccagaagt ttatccagaa gctatcaagg aattagacat agacccaata gacaatccag
    15661 atttagatat agaagaaata agtaaagaca atgggttggt tatggttctt aatgtagaag
    15721 ttaaaccaga gtttgaatta ggaaactaca agggtataga aatagctaag gttgagaata
    15781 ctgtaagtga cgaaaatgtt gatgctaaac tacaagaaat ggttgaaaaa aatgccagat
    15841 tagtaagtgt agaagacaaa gctttagaag atggagatac agctataata gacttcgaag
    15901 gttttgaaaa tggtgtagct tttgatggtg gtaaaggtga aaattataac ttagttatag
    15961 gttcaaacac atttatacca ggatttgaag aacaattagt aggtaaaaaa gctggtgaag
    16021 aagtagaagt taatgttact ttcccagaag aatatcactc acaagattta gctggaaaac
    16081 cagttgtatt taatgtaaaa ataaatgatg taaaagtaaa agaattatct gcacttgatg
    16141 atgaatttgc taaagatact agtgaatttg attctttaga tgaattaaaa gcagatgtaa
    16201 gagctaagtt agaagaagaa gctaaaaata gagcagatgc agaaactaga aattcagtag
    16261 tagaaaaagt tgctgaaaat acagaaatag aaataccaga agttatgata caacatcaaa
    16321 tagataatat gttaaatgaa cttaactacc aattacaata tcaaggattt ggattacaac
    16381 aattactaga gatgactggt agaactatgg aagaattaag agaagagaga aaagaagatg
    16441 ctaaaaaatt agttaagtct tctttagtat tagaagcaat aactaaagct gaaggaattg
    16501 aagcaacaga agaagaattt aaggctgagt tagaaaaaat ggcttctgct tacaatatgg
    16561 aagtagaaaa aatagaagct tctttaagag atgctgacaa agaagatata aaaggtcaaa
    16621 taaaaataag aaaaacaata gatttattag ttgataatgc aacaatagct taataaaata
    16681 aacgctaact aatatagttg acgttatgaa atgtttagta tataatgatg tgcaaatata
    16741 atttagttaa atgcgtataa taatttacag tagcttaaaa aaagctactg taaattatat
    16801 agaataaagg gggtatgaaa ccatggcatt agtacctgta gttgttgaac aaacaggaag
    16861 aggagaaaga tcttatgata ttttttctag actattaaaa gacagaataa tcttcttagg
    16921 agatcaagtt aacgatgcca cagcagggct tatagtagca cagctattat ttttagaagc
    16981 tgaagaccca gacaaagata tacatttata tataaattct ccaggaggaa gtataacttc
    17041 tggaatggct atatatgata ctatgcaata catcaaacca gatgtaagta ctatatgtat
    17101 aggtatggct gcttctatgg gagcattctt gttggcagca ggagcaaaag gaaaaagact
    17161 agcactacca aatagtgaaa taatgataca tcaaccatta ggtggagctc aaggtcaagc
    17221 aacagatata gaaattcatg caaagagaat tttaaaaata aaagaaactt taaatgagat
    17281 attatctgaa agaactggtc agccattaga aaaaattaaa atggatactg aacgtgataa
    17341 cttcatgagt gcactagaag ccaaagaata tggtttaata gatgaagtat ttacaaagag
    17401 accgtagata gagagaggtg ttaatatgtc taaatatgaa gaaaaacgac aattaaagtg
    17461 ttcattctgc ggtaaaaatc aagatcaagt aagaagatta attgcaggtc caaatgtata
    17521 tatttgtgac gagtgtgttg aactttgtga cgaaattatt caggaagaaa tagaagatac
    17581 tatagatgaa gatactacat ctttaccaaa accaaaggaa atgatggaga ttctaaatga
    17641 ttatgtaata ggtcaagaaa aagctaaaaa agctttatca gttgcggttt ataatcatta
    17701 taagagaata tacagtaaaa aatctagtag taaggatata gaaattcaaa agagtaatat
    17761 acttttatta ggacctacag gttctggaaa gacattactt gctcaaactc tagcaagaac
    17821 tctaaatgta ccttttgcaa tggcagatgc tacatcattg actgaagctg gatatgttgg
    17881 agaagacgtt gaaaatatac ttttaaaact tattcaagct gctgattttg atatagaaaa
    17941 agctgaaaga ggtataatat atatagatga gatagataag attgcaagaa aatctgaaaa
    18001 cccatctata actagagatg ttagtggtga aggtgttcaa caagcacttc taaaaatatt
    18061 agaaggtact gttgcaaatg ttccaccaca aggaggaaga aaacatcctc atcaagaatt
    18121 tttaaagata gatactacta atgtattatt tatattaggt ggagcttttg atggattaga
    18181 gaagataatt caaaaacgtg gtggagataa aacactaggt tttggagcta aaatagaaag
    18241 taaaaaagaa ttagatttag ggaagctgta tgaaaaagta cttccagagg atttattaaa
    18301 atatggtata ataccagagt ttataggaag aataccagtt ttagctactt tagagttgtt
    18361 ggatgaagat gcattaatgc agatattaca ggaacctaaa aatgctctag taaaacagta
    18421 taaaaagtta ttggagttag atgatgttga gctagaattt gaagaaggtg ctcttcgtgc
    18481 aatcgctaaa aaggccatag aaagaaatac aggagctaga ggacttagaa gtatagttga
    18541 aagcgtaatg atggaaacta tgtttgaagt accatctaga gataatataa agaaggttat
    18601 agtaacagag aaatctgtga acgaagactc agtaaatcct atcatagtac ttaaggatca
    18661 agaggaaagt gcataagatg atgaaaagga gctttaaaag ctcctttttt tatgttaata
    18721 tgctaaatat aaaataggta tcattattat aaaaatgtga gtaatatacc tagaatacca
    18781 aagacgaata caattgcaat ggaaccaatt tttcctttat ataaaagata aaatgatgct
    18841 ataaatgcaa ttattccata taaatcccaa ttaacttctt tggcagatga aaaagctgct
    18901 gataatatga atcctagagt aattggtcta agaactgtca ataagttttc taccttttta
    18961 ttatttttaa atttagaaaa taactttgat ataatagtta gacctattaa tgcaaaaatt
    19021 gtaacaccaa tagttgcagc tagagaacca aagaagccag cagtcttaaa tcctacaaat
    19081 gtagcactat ttattgctac aggacctggt gtagattgtg atatagcaag taaatccaaa
    19141 aactcttgat tagttataaa gtgatattta gatataaatt cttgctggat aaatggtagc
    19201 atggcataac cgccaccaaa actaaatgcc cctattttaa ggaatattaa gaagagtagt
    19261 attatttctg acatagacta tatacacctc ctccaattat taaataaata gggcttatat
    19321 tgaatacacc aacagataaa actgctataa ttaatagtag tatgttagtt ttagactttg
    19381 gtagtttctt gaacatgctc gtaaaagaat acaatataag agctattaca acaggcgcaa
    19441 ctcctttaaa aaatccatct aaaattttag atgatgaatt tttaaagtat agatacgata
    19501 agactaagac tatacaaaat gatggtaaag cagagcctaa ggtacatata agaactcctg
    19561 gtataccata aagtttatat cctaataata cagatatatt aactgccagt actccaggat
    19621 aactttgagc tatggataaa taatcaataa attcttcctg ctctaaaagt ttttgtttgt
    19681 ttacaagttc atcttgtata agaggaacca tagcgtatcc accaccaaat gtaaacgcac
    19741 caatttttaa aaaggttaaa aacaatttca acataataga aacctccaaa agataaataa
    19801 catctaagta tactaatatt aatattatat cataataaaa agataatata gtatgatagt
    19861 attctaagaa tttatcatta atgttgaaaa ttaacttgtt caaatatata atattaaaag
    19921 caaagcaatt agactagaaa aggagtaatg ttgatggaac aaaattatac taaaatagat
    19981 catgaattac cattaattcc tctaagagga ttagctatat ttccatatat gatattaaat
    20041 tttgatatag gtagagagat atctcttaaa gctttagacc aagctatgat ggatgaagaa
    20101 cttatatttt taacttctca gaaagaagca gaggtagatg agccaggaga agaggatttt
    20161 tatcatgtgg gaacaatctg taaagttaag caaatgataa aattaccagg agatacagtt
    20221 cgtgttttgg ttgaaggtgt atcaaggggt agagtaaaga aaatagaaca agaagatggg
    20281 tatttcagag cagtaataga agaaatagta tttgatagtg ataatttgga tagtgaaaca
    20341 gaagttgaaa ttgaagcatt tgtaagaaat gtatttgatg cctttgaaga gtatataaat
    20401 attggaaata gggtatcacc agaaatatta atatctttag ctgatataga agatgtagat
    20461 agatttatag atacgatagc tgcaaacata tatctaaaat caagccaaaa acaagaaata
    20521 ttagaagaat ttgatattag aaaaagactt gaacttattt attctatatt attagaggaa
    20581 atagatatat taaaaataga aaagaaaata actttaagag taaaaaaaca gatgaataag
    20641 gttcaaaaag aatattatct tagagaacaa ttaaaagcta tacagaaaga attaggtgaa
    20701 gaagaagata taaactctga agcagatgag tatagagaaa agcttaaaaa aataaaagca
    20761 cctaagacaa ctaaagagaa aatagaaaaa gagatagata aattttcaaa aatatcttct
    20821 atgtctccag atgtatctgt aagtagaaac tatttagata ctatattctc attgccatgg
    20881 aataaagaga caaaagataa attagatata actaaggcaa aagatatatt agatgaagac
    20941 cattatggat tagaaaaagt taaggagaga atactagagt atctagctat aagaactctt
    21001 gctaagtcac ttaaagggcc tataatatgt ctagttggtc ctccaggaac aggaaagaca
    21061 tctatagtaa aatcaatcgc cagagcatta aatagaaaat ttgtaagaat atctttgggt
    21121 ggagttagag atgaagcaga aataagaggt catagaagaa cttatgtagg ttcaattcca
    21181 ggaagaataa ttaatggggt aaaagaagcc caaacaaaaa atcctgtatt cttatttgat
    21241 gagatagata agatggcagc agattataaa ggtgacccag cttcagcaat gctagaagta
    21301 ttagacccag agcaaaataa ggattttgtg gaccattact tagaaatacc ttttgatttg
    21361 tcaaaaatac tatttgttac aacagcaaat agtctaagta atataccaag accattatta
    21421 gatagaatgg aagtaataga agtatctgga tatatagaag aagaaaaatt aaatatagca
    21481 aaaaaatacc tacttccaaa acaaattaaa gaacatgctt taaaagaaaa ctttataaaa
    21541 atagatgatg aaactttaag aagtataatc aaccattata caagagaagc tggtgtaaga
    21601 acactagaaa gaactattgg taagatttgt agaaaggttg ccaaaaaata cgttgaagac
    21661 ccaactcttg aagaggttgt tataaataag tctgacctag agacatatct tggaaaagac
    21721 atgtttaagt atcaacttgc agaagttaac cctcaaatag gattagtaaa tggtcttgca
    21781 tggacagaag ttggtggagt tacacttgaa gtagaagtaa acgtacttaa aggtaaagga
    21841 gaaattgtat taactggaaa attaggagat gttatgaagg aatctgcgaa gacaggtatc
    21901 tcttatataa ggtcaatagt ggacaagttt gatatagacc ctgaatttta taagacgaat
    21961 gatattcata tacacattcc agaaggtgct gttccaaaag atggaccatc agctggtata
    22021 actatggctc ttgcagtaat ctcagcactt actaaaagac ctgtgcctgg aaatatagca
    22081 atgacaggag agataacttt aagaggtaga gtcttagcag taggaggagt taaagaaaag
    22141 ttattagcag ctcatagagc tggtataaca aaagtattaa ttcctaaaga atgtgaagct
    22201 gatttagatg aaataccaga aaatgtaaaa gaaaaaatgg agtttgtgct tgtagaacat
    22261 atggatgaag tattagaaca agctttatta aagagtggtg aaaataatga aaattagaag
    22321 ttcagagata actatgagtg ctgtgaataa atcacagtat ccagcagaag gaatcccaga
    22381 gatagcattg gctggtaggt caaatgttgg aaaatcatca ataataaata cattgcttaa
    22441 cagaagaaac ttcgctagaa ccagtcaaac accaggaaaa actagaacta taaattttta
    22501 tctaataaat aatgaatttt attttgtaga ccttccaggc tatggatatg caaagatagc
    22561 taagtctgaa aaagaaaagt ggggaggtat aatggaacga tatctagaaa gtagacaaga
    22621 gttatgttct atattcctac ttgtagatat tagacatgag cctacagcag atgataagct
    22681 tatgtatgaa tggataaagc actttggata taactgtgta gttattgcaa ctaaggcaga
    22741 taaaatatct agaggacaat atcaaaaaca cataagtata ataagaaaaa aattgcaaat
    22801 ggaaagtagt gaaaaagtaa ttcctgtttc ttcactaaaa aagactggtg ttgaagaatt
    22861 atgggaagag attgtaaatc aatacaatca acatggatat gaaataacag tagattaaat
    22921 ttaaaatgtt aaatattgac atgagattta tttgaataat aaatgtaata atagttagtt
    22981 ttaattatat taatattaaa gcaaaatata ggatgcctta aaatagtttt aatgaacttt
    23041 ttttggcatc cttattgttt ataaacaata aatatcagta attttgttaa atactaataa
    23101 attttagtaa ggtatgggaa aattagtata aattagttca actaaaaaat tattgactta
    23161 tgtagtcagc aataaatagc atttaagaaa tagatttatc tgtttttctc ataaagaaaa
    23221 cagttagtat aagccctata aaacatactg atgctgcaat ccaataagac tgattgctag
    23281 ctataacata ggctgatgtg tgacttttaa acttttgatt gtatgtaaga ctttgtgcaa
    23341 aaaaactact aaaaaatgct gttccaagag caaatgataa attacgaata gttgcaagaa
    23401 aactgcctcc gtatccctgt ttttgaggag gaacatctgc taaaatacta ctattgtttg
    23461 gtgaactaaa tgctcctagc ccaagtccaa aaataactaa acatacaatt aaaagataca
    23521 atggagaatc tgcttttaaa agtgccattc caactagtga gatggtcata aatgaaaaag
    23581 atgctgttag aatgtttttt gcaccgtgtt tatcagaaag tgaacctgca ataggtgcta
    23641 taagtaccat aataagtgga tgtacagtca ttacataacc agccatcata ggagataaat
    23701 tttttaattg ttctaaataa aatggaagta gaaaagatac tgccatttga ggaaaataac
    23761 aagctactcc aatgatattg cctacagtaa agtttctatt tttaaataga ggtaggtcaa
    23821 taaagctttg ttcaaatttt acttctctta atgccaacaa taagaagatt ataagtgctg
    23881 ttatacctaa atacaatctt gtattaccct tctcattcat tgcaagtaat ataagcgtag
    23941 tgcaagatgc gaaaagtaga agacctggaa tatcaaagct cttgttttgt tcatccaaaa
    24001 caggtttagg tagaaattta atgccaagaa agactgctat aataccaata ggtatattga
    24061 taataaaaat agaaggccat ccaaaatgtt ctaaaattat acctccaatt acaggaccac
    24121 tcatatttcc tataccaact acagctccag tgattcctat agcttttcct ttttcatttg
    24181 ctggaaatgc attggaaact ataccaagcc cagtagccat taaaatactt gctccaactg
    24241 cctgaaagat tcttgaagaa attaatgttg ataaattgtt tgacatacta cacagaaaag
    24301 aacctattgt aaatataaaa aatcctaatg tgtatagacg atttgaacct actttatcgc
    24361 caagttttcc gaaaaataac atagtagctg ttacaacaag catataagct gttacaaccc
    24421 actgaatttg tgacatgtta attttaaagt cagcagcaat tgtaggattt gcgatattta
    24481 gtatacttcc atcaagagta gacataaata caccaataca gatgataatc aatactatcc
    24541 atttttgttt catgataaca ctccaatctt aatagatttt ataagtacga tataattgta
    24601 aaataaacaa caaaaaaagc acctgtatat taggtacctt gaatgtgtct aatttcatga
    24661 ataaatacgg tagttgaagg atttgtatag attactcatg taaaattaca taaaggtact
    24721 catatactat tttcttctac catccagatt ttactgtcgg ctttggaata tgaccaaatt
    24781 atgccataag gctcgcgggc ttttaccgcc gatagggaat ttcacccgtt tatgaagaaa
    24841 aacagatgta ttttgtaata ttattttaac ataaaattat aatggaaaca attggattta
    24901 agtaatatcc agctgtatta caatacaaat atttcaatat ggacacttaa attgtattta
    24961 tagatagaat aaggaacagt atgagaatct ataataatta gataaaatta acctgttatt
    25021 agtattagat ggtaagtttt aaatttatca aaatcagaaa gctgtaataa aattcttata
    25081 atatgaatat cttaattaag agatattgta aattatgagg aggcgctaat atggaaggaa
    25141 aggtaaagaa ggcattcccg ggtggaaata catcacaagg ctcatattct ttttttgact
    25201 atataattcc tgaaaatgta aaaagagttt tttgtttaaa gggtggacct ggagtaggaa
    25261 aatcctcact tatgaaaaaa atttataaag agtttttgca tagaggatat gatattgatt
    25321 tatatcattg tccttctgac ccaagttcgt tggatggttt agttataaag aaattaggcg
    25381 tggtacttat ggatgcaact gccccacata ctatggaccc aaagctacct ggagctttgg
    25441 atgagataat taatttaggt gattactgga atacagaaga attagaaaag aataaacaag
    25501 aaatatcagt ttatgataaa gatattagta attcatttaa aagagcattt aagtttttaa
    25561 aatcagcaga accaatcttt agagacatag aagaaaaata ttctgattgt atggattatg
    25621 gaaaagtaaa tttggtcaca gaagaattta taaaaaggtt atttgatgga gttaaaagca
    25681 caggaaatct aaaaagagaa aagcatttat ttggttcagc tataactcct gtagggtgtt
    25741 tagattatac agaaaacatt ttaaaagatg taaagaaagt ctattattta gatggagaaa
    25801 taggaactgg gaaaactaca ttattaaaca aagtatataa gaaagctatt gaaaaaggtt
    25861 taaatgtaga agtttatcat tatccactta ttccagagaa aattgaaact gtactattaa
    25921 ctgatttaga tataggcata actataagta cgacttttaa agaagaagat acaattgatt
    25981 taaatcagtt tttaaataga gaaaaactac ttaaatatga agaagatatt aaatttgatg
    26041 aaaaagtttt agatgattta atttgttggg cagtactaaa tcttaagaga gccaagtcta
    26101 aacatgatat tatagaaagt tactatagtc ctaatatgag atttgatgaa gtagggaaat
    26161 taagagataa gttaattaaa agaattttaa aatatgagga aaacttataa acgcatatgt
    26221 ttaaaattaa cgttagtaat aatataattt ttagtaaatt ggtgtagctt tgaggctatg
    26281 ccattttact ttttttgcta aaaaatgata ttaataatct ccaaagccat tatttgtttt
    26341 tcataattat tatttccttt cctaatttat gtttttttat aatatcacaa caatgtttat
    26401 tgaaagttta tataatttta agaacatcta aaaatgcata cttgatattg gtaaattagt
    26461 gataggagtt caatgtatga tattatatac taaagatgaa ttttaatata taactttgat
    26521 ataataagca tatgtaaatg tttaatattt aattaaagaa aattatcata taaaaataaa
    26581 tttgtaagga gcaatacagt atgaaaaaac aatatgtgtt tataatacta gcaagtacta
    26641 ttttaggtat acttgttgga accatattta gagattatag caatgaagaa acagtttata
    26701 caagcaaaga caagtcaata agaaaagaaa taaaaatgac aagaaaatct ataaaagaat
    26761 taaccaaaga gaaagacaag ttagataaag aaattaattc aatgaaacaa aatcaaaaaa
    26821 attctgaaga aatatcagaa atagaaacac ttagagatag tctatcatat tcagatgtaa
    26881 agggtagtgg gataatatta agtatagatg ctatagatga aaaaacaggg aatatagcta
    26941 atagtataga ctataataag attcttgtaa aagttataaa tgaattaaaa gtaaacggag
    27001 gaaaatacat agaaataaat ggtcaaagaa ttaatcaata ttcagaagtt gtacttgcag
    27061 gaagccatat aaatataaat ggagttcctg tagcacaacc atatgaaata aagtgcattg
    27121 gagataagga gaaactatct aattatgtag ataaaggtag taaatattta gatagcatac
    27181 aaaccaatta tccaatgaaa ataagcataa aaactgaaga aaatatctct atgaaaaaaa
    27241 tgaatatacc taataaatta aagtatataa agggtgatta agattatatt agaggaaagc
    27301 agtttgccaa tgttaatacc agaaaaactt gcaaaaaaat ataatgtatt tccagttgag
    27361 tttcaagatg ataatttagt tgtagaaata gaagatgagg atatttatgc tctacaggac
    27421 ttgagattag ctacaggtaa agaaattata ttgaagaagg agaaaaaaga acttataagt
    27481 gaaaagattg aaaaatatta taatagcttt gaacttgatg aggattatgc aaaaaaatta
    27541 tttgaaaatc ttttagaaaa agctataaaa gaaaacgcaa gtgatataca tatagaacca
    27601 tttaaagaac atttaatagt gagaatgagg gtagatggag aactaaagga agtatcaaag
    27661 tttttagttg atgtctatcc atcattgtct acggttataa aattaaaggc atcaatggat
    27721 ataacagaaa aaaggcttcc tcaggatggt agggtggata taaagttagg tggaaattta
    27781 attgacatta gagtttcatc aataccaact gtctatggtg aaaaaatagt tttgaggata
    27841 cttaatagag atactttttt aaaagacaaa ttagaacttg gtttttcaga ggaagctata
    27901 aaatctataa atcgtataat aaataaaaga gcaggaatat tattagtgac aggtcctaca
    27961 ggtagtggaa agactacaac agtatattcc ttattaaatg atttaaaagg aataaataaa
    28021 aatattatga ctatagaaaa tcctgtagag tataagatgg atggcataag tcagattcaa
    28081 gtaaattcaa aagtagggtt gagctttgat gtaggtctta gagcgatatt aagacaagac
    28141 ccagatataa taatggtcgg agaaataaga gatgcagaaa cagctaagat agcagtaaga
    28201 gcagctataa caggtcattt agttataagt actttacata ctaatgatgc agtatcttcc
    28261 atagctagat tactagaaat gcaaatacca ccatatcttt taaatgcctc tcttataggt
    28321 gttatatctc aaaagttggt tagaaaagtc tgcaatcatt gtagccatga gattatgata
    28381 aaagataatt ttagtggaga tgtaaatact aaggtagctg tagggtgtga aaaatgtaat
    28441 gataaaggat actttggaag aacagcaatt tatgaaatct tagaaattaa tgatgatata
    28501 aagacatgta taagaaatat ggaggattct agtactatta aagatgtagc acaaaataat
    28561 ggaatgataa catttgaaga tagttgtaaa agactaataa atgaaaaaat aacgacatta
    28621 gaagagtgtt tagtagtaaa tgaaatgatt taactgaatt gtatagttaa acttcgtttg
    28681 gtcagttgtc tttatttaat ctattttaat taagatgttt gagtataaat tatatttaag
    28741 tgaaataaaa ttaaatgcat taaattagaa aatattaagt ttatttaatt gtaattaata
    28801 taatttggaa ggtattatag tacatgagat tatctaaaaa tatattttat aatttagaaa
    28861 agttttattt agtattaaat agaatcagtg ataaagaaat tagagtaatt tgcaaagaga
    28921 taggcatatt gctggaatct ggatgtgaaa ttacaaaaat atttgaaatc attgagagtc
    28981 agtcaagtaa aaaagtaaag aacttgctta gtatagtatc aaatcatatt caaaaaggta
    29041 actcaatagc agaatcattt caaataacag gtatattttc taaattcttt ataagtatga
    29101 taaaagcagg agaaactagt ggaaacttag atataataat gagtgattta tctaattatt
    29161 atgataaaga atacaaatta aaaatgaaaa taataactat ttcaatatat ccaataatac
    29221 ttataatatt atctatctta tcaatgttgt ttatattcgt atttgtaata cctaattttc
    29281 aggtggtttt taccaacaat ggaattgaac cacctttaat aactagagtt ttaatgggaa
    29341 tatctacagt ggttacaaat aacttagctt atataatatt tagttttata ttgttttcaa
    29401 taggaagcat ttatttcttt ataacaaatg ataatataaa aaaattgatt aattcgttaa
    29461 aattcaaaat accttttata aaaaaaatta atcagttagt agctacaaca agattttcaa
    29521 gggcattgtc cgttttaatt agcagtggaa tacaaattgt ggaagctata gatatgtcag
    29581 ctagtgttat agacaacgaa tttatttatg aaaggttaaa gatttctaat aattatataa
    29641 aaaaaggaaa tagcataggt aaatctttga atttagcaaa tatttttcca aaattattta
    29701 tttcaatggc aaatacagga gaagaaagtg ggaaactaga taaaagtcta aatgtaataa
    29761 ataaatttta tgaaaatgaa ttaaataaca aaatagagca aataatgaaa tttatagaac
    29821 ctatgctaat tgtatttatg ggattaatta ttggtacttt tattttagca atggttgttc
    29881 ctatgtttga tgcaattact aatttttaga gagaaaaata aattttatta gatgaaactt
    29941 ttgaaaagga gagaatattt aattgataaa tttgataaat aaaaaacgaa aaggatttac
    30001 acttgttgaa atgattgtag tagtaactat tttaggcgtt atatctagta tagcattagt
    30061 taagtatagt aaggttcaag aaagtgccaa attaaatgca gactatacga atgctgctaa
    30121 tatagtaacc gcagctagca tggcaattaa tgatgatgaa aagacaatag actctctaag
    30181 tgtagagaca ttgaaggaaa agggatacct aaatactgtt ccagttcctc agagtacatc
    30241 aggtaaattc gaacttgtca taaatgatag cggaacagat ataagcgtaa atataaattc
    30301 taagcaattt tatccaaaat aatattttat attgattgct gtaagtttta aaattatttt
    30361 tatttaagaa ggagatggct ataattaaaa aaattctaaa cttagaaata aatgatgatt
    30421 atataaggct tgtattttta agaaaaagtc ataaaaaagt ttttatagat aaatcaatta
    30481 taaaagagat taactttgat ataaaaaatg atagtaattc gattgagaag attaaatata
    30541 tagaagatgt agttagattt gtaaaagagg agattggtaa atcaaaaata ttatataaaa
    30601 atttatactt taacattcaa attcaggata tagtcattag aaatgttgaa gttctaaata
    30661 ctaagaaaaa aagagacata ttaagcatga ttgaatttga aataacacaa tacattccaa
    30721 taaacataaa taactattca ataaaatata aagttataaa ttctaaaaat gataaattga
    30781 atatacaagt aattcttttc ccaaagaata ttattcaatt aattaagaac atttcagaaa
    30841 atctaaatat gaatccaaag actataaata taaattttga tatattacaa aagcttataa
    30901 acttaaattt aatagagaat ttttcagaag aaggggtatt tatagaatgt aaaaaaagtc
    30961 agttaattat aaatataaca aaagataaaa aaataagaga aacatacttg ctatcaaaag
    31021 agggaaaatc atatgagttc ataaaaagat tattaagggg ttttaagtat gtttattact
    31081 atggtataga aaattgtttt attgaagaat actttaaata tacagataaa tctattgaag
    31141 taaatccatt aagattaaaa gataaagtaa aagtagaaaa taattttgat gcagatttag
    31201 aagacaatag gataaattat ataaatagta taggaatgat tatttaagtg aaagtggata
    31261 aaacaataaa ttttcttaat ggatttaaaa aaactaaacc atgtaataaa atctataaaa
    31321 taataatagt cttaatttta acgtgtattt tatttaaatc atttatgtat gtgaaatcta
    31381 taaatgaact tgaagattat ataagtcaaa ataagctagt ggataaatca aagaacactt
    31441 taccaacaac agataaaact agagagaaat tttctaaaat aaaacttaat gaaataaaaa
    31501 aaatatatga aacaattgga ataaatagta ttactaaaat atcaatagat aaaaataagc
    31561 tagagatgga aggtgtgtgt agtgatttag atgttttaaa caatttaagg aaaataaagt
    31621 acataaaata ttattacata gacagtattg taagagatgg agaatattat agatttagaa
    31681 taaaaagtta gatgggtgat tatttttgag attaaataat agagaaaaaa atctacttaa
    31741 aatattgtta atattagtta ttatattggc aagtgatata gcagtaagtt atttggaaga
    31801 aaacctaact agtttgaaga atgaaaagca aaatataata aataaaataa ataatcaaga
    31861 taattatcaa ataaaggata ataagcatat agatgataaa tatatagaaa gtgttgaatt
    31921 aaataaaaat gaagaattga aaaataattt taataactca tttttagata aaaaaacaaa
    31981 taaagatata gtgttattag tagaaaatga actgagagat attgtgaatg tagaatatat
    32041 taatcaagaa agtagtttag ataatcacaa taaaaaacaa gttaatttag aaataaaagt
    32101 taatggagaa cttgcaaatg tgttgaaact tgaaggtgta atgaagaagt taagactaaa
    32161 taataaactt gaaaagatag aaattaacaa attaaaagtc aataattttg atgttcaaaa
    32221 tattccaaat gaatataaac aagaaaaaga caatattgtt gaatgtaaaa tgacagttag
    32281 agtcatataa caaaaaagaa tagataaagt aaaaaactat tatcaattaa taaaatttta
    32341 tagaggaatt agttttacga taattcattt ctacaagagg gattgttgat gattttatcg
    32401 aaatcctaat taaagttaat atattgtacc aaaatataag tgaatttaat agaatattta
    32461 cttatactat agattgatta ataaaaaaat ggtataataa caaattgtac tttaatttaa
    32521 taataaggga gtggaagtca tattgttagg gagaaatgag ctatgccctt gtggaagtgg
    32581 caaaaaatac aagaaatgtt gtttaaataa agatattgtt tcagaaagag catgcagaaa
    32641 aactgaactt tcacaaaaac aatatacaga tttatattca aagttatatg attattcaag
    32701 acaggataag tttaaagaag aatgtgaaaa agctaaagaa atgttttata taatgcaaaa
    32761 tgatagtgta aatgaaaaat ttgaaagatt ttttaatact tatttaatac aagaccacat
    32821 aatggaaaat aaaaaggtta tgacagttgg ttttcttgaa gaaaatggtg caaacctaag
    32881 tcaatcagaa gtatcaatat taaaaagtat gtttgaatca tacgtaagta tttatgaggt
    32941 aaaagaagtt tctgctggaa aaatagtatt aaaggactgt ttaaatggaa atgaactttg
    33001 tacagaagat gtaaaacttc tgagaagttt taaaatcgga agttgtatga tagctagaat
    33061 agttgatatt gaaggaacta acatattgat agatataact ataagtatat caaatgaggt
    33121 taaagatata attttaaagg atattatgaa tctattcaat caatatcaag atgtatacaa
    33181 agatatgaag tcgttcttaa tttatcatac acatatacta tacaagtata tacaacagct
    33241 tttagaccca agtatagctg aatatttaaa gagagaaaga gataaaaacg atacaaaagt
    33301 taattcagaa aatgaattag ctgaagaaga atgtaaagtt ttagatactc ttaaagaaaa
    33361 tgtagaaaaa gaaaatatag atgcatgtat agagttttgg aatgaatata agaaaaatca
    33421 tgaaaatata aaaggagctg aaaatggttg ggcagctgct gttgaatact atgttaaaaa
    33481 agaagcagga caacctataa ctcaagttca aatatcaaag aaatacgata taagctctag
    33541 tacactagga aagagataca aggatttaaa aatatcttaa ttaattataa aaagctttaa
    33601 gtcatagata aagaatctat agtataatat atgtataatg tttagaaaga ggtattaata
    33661 atgagtctta gtttaggaaa tgcaattgct gtatcaacga actttataaa atctaaccca
    33721 gctgtacctg tgtacaacgg agaagaaata tatgcagtta tttatcatat agaaatagga
    33781 tataataatg gtgtatcacc tgataccaaa caatatataa taatagacta taaagagttt
    33841 gatttatcta tggataaaaa aaatgaacta ataaatattg ctaaagagta ctgtaaatct
    33901 aaaggtattg tagaaaacga aaaccttgaa ataaaccttt tagacaatga agaagcagag
    33961 aacttcttag aaaaggtgtt taataatatg aaggttataa gaggtcttat aactaaaaaa
    34021 ggataaataa atggactata taggaataga aaatataaca ccttatgaaa atacatatga
    34081 atttagtgta tatgaatatg atgatgaaat caccttaggt agtgaaaagt tatatgtatg
    34141 tgaattaagg gttgtattga ttaaagttaa ttctctgtat gttgaaagat tgcataaatc
    34201 agttgaagca atggtcttag taaaaaactt gaaaaaagat ttagataaaa cacttgttgt
    34261 aaacaaaata aagaattttg tgctagatga gatttgggta gaaaatctag taaaagagaa
    34321 tatagaagtt atatttgtag aaagctagga ataaccctag cttttttata ttacaatcta
    34381 agagaggaaa acaatatgtt aagagtatct aatgtaaaac ttggaattga taaagatata
    34441 tctttattaa aagatgttat acttaagaag ctaaaaataa aagaaaaaaa cttagtcaaa
    34501 tatagtatat ataaagaatc tatagatgct agaaaaaaag gaaaaatgga atttgtttat
    34561 tctgtagatg tagaagttaa agatgaaaat aaaatactta actcaaaaat aaaagatgta
    34621 acaaaaataa aggaaatcag atacgtaaat gtacctgtgg gtgataaaaa attaaagaat
    34681 aatcctttgg taattggtag tggaccagct ggcttgtttg caggtctgct tttagctcaa
    34741 atgggttatg aaccaatcat acttgaaaga ggtcttgatg tagacagtag aaccaaagat
    34801 ataagtgatt tttggacaac tagaaaatta aaaaataatt caaacgttca atttggagaa
    34861 ggtggagcag gaactttttc ggatggtaaa cttacaacta gaataaaaga tatacgatgt
    34921 agaaaagtat tagaagagtt agttcagttt ggagcaccag atgaaatatt atactcacat
    34981 aagccacatg ttggaacaga tatattaaaa aatgtagtta aaaatataag aaatgaaata
    35041 ataagtttag gtggtcaagt tagatttaat tcaaaagtaa cagatatagt tgtatcaaat
    35101 gattctatac aatctgtaat aataaatgat actgaaaaaa tagatacaga tacaattata
    35161 ttagctgttg gacatagtgc aagagatact tatgaaatgt tgtattcaag aggtataaaa
    35221 atagttcaaa aaccttttgc aataggagct agagttgaac atccacaaga tttaataaat
    35281 aaagcacagt acaaggattt ttataatcat cctaggttag gagctgcaga ttatagactt
    35341 atagagcatg tttcaaatgg aagaacagca tatacttttt gtatgtgtcc aggaggtagt
    35401 gtcatagcat ctgcatcaaa tgattttgaa atagttacaa atggtatgag tgaacatgca
    35461 agagataaaa taaatgcaaa tagtgcattt ttagtaaatg taacgccaga agattttaag
    35521 agtgataatc cactagcagg tgttcatttc cagcagaagt atgaaaaatt agcttttgaa
    35581 ttaggtggaa aaaattataa tgcaccagtt cagcttgttg gagattttat aaacaataag
    35641 acttctttga atttaggaag tgttgagcca tcatatagac caggatatac acttacaaat
    35701 ttaagtgaat gtttgcctag cttcgtaaca gagactatga gagaagcgtt tatatctctt
    35761 gataaaaaat tgaatgggtt tgcaatgtat gatgctgttt tgacgggagt agaaactagg
    35821 tcatcatcac caataagaat agtaagagat gaaaatactt tagaatcagt gagtattaag
    35881 aatctatatc catgtggtga aggtgctgga tatgcaggtg gtatagttac agcagcagtt
    35941 gatggtataa aatgtgcaga aaaaataata caaaaataca ccaataatac aaaaatttaa
    36001 cataaactta atgatttttt aatatttaac atgaatttaa cattatcact cctataattt
    36061 gataaaatac aataggttat aaaattttta gatacagtaa tctctttaca aggaggaaat
    36121 aatttgaaca ttataataag tctaatgggt ggattaggtc tgttcttata tggtatgaac
    36181 cttatgggag aaggtcttca aaaatctgct gggacaaagc taaaaaagat aataaaacta
    36241 cttactagta acctatttat gggagttttg gtaggtacag gagttacagc agtaatacaa
    36301 agttcaagtg ctactactgt aatggttgtt ggtttcgtaa atgcaggaat aatgacttta
    36361 aaacaggcaa taggagttat aatgggtgct aatataggga ctactgttac agcccaattg
    36421 gtatctttcg acttaactgg tatggcccca gtagcattag gtgtaggtat tatactttat
    36481 ttatttggaa ataagcctag aataaaaaat atagccgaaa tacttatagg ttttggtata
    36541 ttatttacag gtatggattt tatgaagatg gcagtggagc cactaagaga ctatcaagga
    36601 tttactgact tacttgttac atttggtaga tatccactac ttggattatt attgggattt
    36661 gggattacag caataataca aagttcaagt gcatctatgg gtatgttggt tgcactggca
    36721 gcagaaggtc tagtgccatt atcagcagca ctgccaattc tttatggcca aaatattggt
    36781 acatgtgtaa cttcactgtt atcaagtatt ggtgctaaca aaaatgcacg aagagcagct
    36841 atgatgcatc taatcttcaa tgttttagga acggtgatat tcttaatatt cttgaataaa
    36901 ccagtagtaa gcatggttac aagttgggac ccaagcaatg ttgcaagaca aatagccaat
    36961 acacatacat tgtttaatat aatatctgta ttgatacttt taccatttac aaatcttatt
    37021 ataaaactag ctataaagct tgttcctgat agagcaggtg atatagatga agatgaaact
    37081 aaaactatta aatatataga tgatagaatg atagaaactc cttctatagc tcttgcaaat
    37141 acgataaaag aagcacttcg tatgggtgag aaggcgaagg aaagcctaaa tgcatctatg
    37201 gaagctttag tagaacattc aactgaaaaa atagataaga catataggcg agagagatta
    37261 atcaatgatt tacagaaagc tattttaaat tatttactta aactttcaaa agcaccttta
    37321 gatgatgatt caagagaagt tgtggatact ttgtttaata cagtcaatga tatagaaaga
    37381 attggtgacc acgctaaaaa tatagctgaa ttatcgcaag ttgcaataga ttcaaatatt
    37441 tctttctcag aagaaggaca atctgaactt gatgttatgt ataatagagt tgtttcagca
    37501 tatacttatg cattagaatc aatgagaact gataatgttg atttagcttg taaggttata
    37561 aaaattgaag aacaagttga tataatggag aaatcttgta gagctaatca catgtataga
    37621 ttgaacaata atctatgtag tatagaaaat ggagttattt atttagacgt aataagtaat
    37681 ttagagagaa tatctgacca tgctgtcaat atagcacaac aagttatagc aaagagatta
    37741 ggaaatgatt aaaaataatt aatttaaaat aatataaaat ttaaaataga ggataaataa
    37801 aagagaaact ttaaatgata cgtttagagt ttcttttttt gtaaatttat ttgactaaaa
    37861 tttttaaatt tatacatagt tatagcattt agaagaatcg atttcctgat aataaatgat
    37921 gagatatgta tgattttgtg gcaaactttt aaaaaaaaca cctatatttt gtgttataat
    37981 ctctttaaaa cgaatatgct gaaaagaggg atttaaatgg gaaaaataaa ttttgattat
    38041 agtaaagcta cagagttttt ttgtcaaaac gaaatagatg tcatgcaacc atatgtagat
    38101 gttgcgcacg acatgttaca taataaaact ggtttaggaa atactttttt aggatggata
    38161 gaccttccta aaaattatga taaagaagag tttgacagaa taaaaaaatc tgctgataag
    38221 ataaaatcag actcagatgt tcttttggta ataggtatag gaggctctta tttaggttcg
    38281 agagctgcta tagatatggt aagtcattct tttaggaatg gacttaaaaa agagcaaaga
    38341 aaggctccag aagtatattt tgtagggcat aatataagct caacatatat tatggattta
    38401 ttagatataa ttgaaggaaa ggatatttct gtaaatgtaa tatctaaatc aggaactact
    38461 acagaaccag ctctggcttt tagaatattt aaagattact tagagaaaaa gtatggtaag
    38521 gaagaagcaa gaaagagaat ttatgcaaca acagatgcaa gtaaaggtgc acttagacag
    38581 ttagctacag aagaaggata tgagactttt gtaataccag atgatgtagg tggtcgtttt
    38641 tctgtattga cagcagttgg gttacttcct attgctgctg ctggacttga tattgatgct
    38701 atgatgaaag gcgcaaatga tgctagagaa gcatttcaaa atcctgactt aaaaagtaat
    38761 gatagttata gatatgctgt tgctagaaca attttacata gaaaaggaaa agatgtagag
    38821 ttattagtga actatgaacc tcaactacat tatgtaagtg agtggtggaa acagctttat
    38881 ggagaaagtg aaggaaaaga aaataaaggt ttattccctg cttctgtaga tttctcaaca
    38941 gatttacatt ctatgggtca atacatacaa gatggaaaaa ggcttttatt tgagacagta
    39001 cttaatgttg aaaactgtaa gagaaatata actataagtt cagaggaagt agacttagat
    39061 ggtttaaact atttagcagg taagactgta gattttgtaa atcataaagc ttttgaagga
    39121 actctattgg ctcatacaga tggaaaagta cctaatttag taataaatat acctcaacta
    39181 gatgagtata attttggata tttagtttat ttctttgaga aggcttgtgg aataagtgga
    39241 tatttattgg gagttaatcc atttgaccaa ccaggagtag aagcttacaa gaaaaatatg
    39301 tttgctttac taggcaaacc aggttatgaa aaagaaaagg aagagctaga aaaaagactt
    39361 aaataatttt aaatagttaa ggtttaatta agattaaaat aatataatga aatcatcgaa
    39421 aaggagttta ctcaaaatag atgatttcat tttttttatg aaaaattggg aaaaatatat
    39481 tttgataatt tatattatat taagataggg agtggtatga gatgtcaaga agaaagaaag
    39541 ggataagttt agtaatatta gttgctataa taagttcaat acttagtagt tttctaacaa
    39601 taattttagt taaggacaat ttagtaagta aatctacagg aagttctacg ccgatagttg
    39661 taaatgatga tggtaagagt caaaatattt atcaagcagt tgcagaaaaa gctacgccat
    39721 ctgttgtagg tattacaaca actagtgttg atactagtaa tatgtttgca ataccaactg
    39781 aaacacaagg tgtaggaact ggaatcatag ttgattcaaa tggatatata cttactaatt
    39841 ctcatgttat atcagatggt caagctacaa gtgtaaatgt attattcaat gatggttcaa
    39901 caacttcagg taaagtagta tggtttgacc aacaacttga tttagctata gttaaggttg
    39961 ataaaactgg actaacacca gcagaatttg ctgatagtga caaagtaaaa gttggagata
    40021 tatcaatagc aataggtaat cctcttggat tagattttca aaaaacagtt actcaaggga
    40081 taataagtgg attagataga actatacaaa cagaaaaaac taatatgact ggtttattac
    40141 aaactgatgc aagtataaat gcaggaaata gtggaggacc attgttaaat caaaaaggtc
    40201 aagtaattgg tataaacaca gcaaaagctt ctcaagcaga aggattagga tttgcaatac
    40261 caatcaatac agctaagagt atagttgaag aagtaatcaa aaatggaaaa tatgaaaaag
    40321 taactttagg cataaaaggt acagatgtat caaattatga agcagcaaca ggtacaaaat
    40381 taagtactga taaaggtgtt tatgttgcag aagtaatatc tggttcatca gcagaaaaag
    40441 ctggagtaaa ggtaggggat attataacta aagtaggaga tacagatata acaggtatga
    40501 atgatttaaa taaaaagttg tatactttct caaaaggtgc tagtacaaaa ataacagtaa
    40561 atagaggcgg aaaagcagta acaataaatg tgaatttcta aatatgacca gaaagataga
    40621 gactacatgg taaagtgtag tctctatttt tatttaattt ttatagtata tacatctaaa
    40681 ttaattaaaa tgatgtattg tttatagttt attgctatat tttcttataa aaataaattt
    40741 aataaatatt taaatttaat ttacaaaaac aatataaata tcataataaa ataaaatttt
    40801 ttaatattac taaaaatata tagacaaaat aataacgaat atagtattat gtaattgtag
    40861 aaatgctttt cataggtata ttctatgaag atgaattgca taaaaatgaa aaaatggaat
    40921 ggatattatt taggggtgaa aaatcatgaa tccattagtt ataaacttac aaaaatgtag
    40981 catacatgat ggaccaggga taagaagtac agtatttttt aaagggtgtc cacttgaatg
    41041 tgtttggtgt cataatccag agagtcaaac atatacaaaa caagttttgt ataatgaaga
    41101 aagatgttca aaatgtgaag catgtataaa tatatgccct cataaggcaa tttacaaagg
    41161 tgaaacaaaa atatgcttag accaagataa gtgtgagttt tgtgaaactt gcttagacta
    41221 ttgtgtaaac aatgcaagag agattgttgg tcaagagtat tctgtaagag atttggttaa
    41281 agagatagag aaggatagaa ttttctatga agaatcaggt ggtggagtaa ctctatcagg
    41341 tggagaagta atggctcaag atatggactt catttgtgga gtaataaaca tgtgcaaatc
    41401 aaagggaatc catgtagcaa ttgatacatg tggctatgca aagtcagaaa attatgaaag
    41461 agttgctaaa tgtgcagatt tgtttttata tgatattaag cttattgatg aagataagca
    41521 cataaaattt acaggaaaat caaatgactt aatactgaaa aatgttaaga ttttaagtga
    41581 attaggggct aacataaata taagaatacc attaattgta ggagtaaatg tagatgatga
    41641 aaatttagag gttaaaaaaa tgatagagtt tttaaagcct ttgaatatac aagcagtaag
    41701 tcttcttcca tatcataata ttggtaaaca caagtatgat aaaatttata aaaagtatga
    41761 aggagaagaa ctacaaagac catcagaaga aaaattagag gaaataaaaa gactgtttga
    41821 agcaagtaat ttcaatacta agattggagg gtaataaaat ggcaagagga acttttgaga
    41881 gaactaaaaa attaagagaa gaaagtatta atgcagaacc acacatatct attgaaagag
    41941 cagtacttat gacagaagct tataaaaaat atgagggaag tgtagagatt cctgtacttc
    42001 gtgctttatc atttaagcat tatattgaga atagaactct tagcataaat gatggagaat
    42061 taatagtagg tgaaaaaggt gattctccta atggagcacc aacatatcca gaaatttgtt
    42121 gtcatacaat ggaagattta gaagtaatgc acaatagaga tataataaat tttagcgtat
    42181 cagaagaagc tagaaaaata cataaagaag aaataattcc tttttggaaa aagagacaaa
    42241 caagagataa gataataaat gcaatgacac ctgagtggtt agcagcatat gaagcaggta
    42301 tgtttacaga gtttatggaa caaagagctc caggacatac agtttgtgga gatacaatat
    42361 ataaaaaagg atttttagat ttaaagaaag acatagaagc aagattaaaa gagctagatt
    42421 ttcttaatga tttagatgca tataataaaa aagcagattt agaagctatg gcaatagctt
    42481 gtgatgcaat ggttatatta ggtaaaagat atgctgagaa agcaagacaa atggcagaag
    42541 aagaaacaga tgaagctaag aaaaaagatt tattactaat tgctgaaact tgtgatgttg
    42601 taccagctca caaaccagaa acttatcatc aagctataca aatgtattgg tttgttcata
    42661 taggggttac tactgaactt aatatatggg atgcttttac tccaggaaga cttgaccaac
    42721 atctaaatcc tttctatgaa agagatgtag aaaatggaat actagataga gatagagcac
    42781 aagaattact tgaatgttta tgggttaaat ttaacaatca gccagcacca ccaaaagttg
    42841 gtataacatt aaaagaaagt agcacatata cagattttgc aaatataaac actggtggaa
    42901 taaatccaga tggtcaagat ggtgttaatg aagttagtta tataatactt gatgttatgg
    42961 atgaaatgaa attgatacaa cctagttcaa atgttcaaat aagtaaaaag actcctcaaa
    43021 aattcttaaa gagagcttgt gaaatatcaa gaaaaggatg gggtcaacca gcattttata
    43081 atactgaagc aatagttcaa gagcttatgg aagctggaaa aactatagaa gatgcaagac
    43141 ttggtggaac cagtggttgt gttgaaactg gatgttttgg taaagaagca tatgttctaa
    43201 ctggatatat gaacattcca aaaatacttg aattaacatt aaataatgga tatgacccaa
    43261 ttagtaaaaa acaaataggt attgaaactg gagaccctag aaatttccaa tcttatgagg
    43321 aactatttga agcatttaaa aagcaattac attatatgat tgacataaaa atagaaggaa
    43381 atgctgtgat agaaaatata tgtgcaaaac acatgccttg tccacttatg tctacaatag
    43441 ttgatgattg tatagaaaaa ggcaaggatt accaaagagg tggagcaaga tacaacacta
    43501 gatatataca aggtgttggt ataggaacta taacagatag tttaacagca ataaaatata
    43561 atgtatttga taagaaaaaa ttcgatatgg atacattgct taaagcactt gatgctaatt
    43621 ttgaaggata tgaagctata cttaatttag tttcaaataa gactcctaaa tatggaaatg
    43681 atgatgatta tgctgatgaa ataatgcaag agatattcaa tgcatattac aatgaggtaa
    43741 ctggtagacc aactgtttgt ggtggagaat acagagtaga tatgttacca acaacttgtc
    43801 atatatactt tggagaaatt atgggagcaa gtccaaatgg tagactttgt gcaaaaccag
    43861 tttcagaggg aatttcacct gaaaaaggtg gagatactaa tggacctaca gcagttataa
    43921 aatcttgtgc taagatggac cacataaaaa ctggtggaac attattaaat caaagatttg
    43981 caccatcagt agttcaagga gaaaaagggt tagataatat ggctaatcta gtaagagcat
    44041 actttaatat ggatggtcat catatacaat ttaatgtatt tgataaaaat gtactgttag
    44101 aggctcaaaa gaatcctcag gattataaag acttaatagt tagagttgca ggatatagtg
    44161 accatttcaa taatttaagt agaacactac aagatgagat aataggaaga actgagcaaa
    44221 cattctaggt ttgcttgtaa ataaaacttc ttactatata aaatcgtaac tgatataata
    44281 aagtataaat ggtttgattc catttttaaa attttagatt aattatgagc taactttcta
    44341 aataggaagt tagctcgtta agtttaatga aaaaatcgtt aaatttatat ttagtaaatc
    44401 atacacatat tagtaagtaa tacatacaaa agaataattt attatagtta taataaggtt
    44461 atattttata aaaataagat aattttaagc agttaaaata aagtcaattt agctacttta
    44521 aaataaaact aatagaaaaa aggagatttg atatgaaaac tttaggattt attggttcag
    44581 ggaatatggg aagtgcaatg ataggtggca tagtacagtc aggattagta aaggcagaag
    44641 atataactgt atcagattta agccaagttg ccttagataa agtaaaagaa aaatttggag
    44701 taaatactac aactgattca aaggtagctg ttacaaattc tgatgtggta atagtagcac
    44761 ttaaacctaa tatatgcgaa aaggctttaa ctccacttaa agaattaata gatgcaaata
    44821 aaataatagt atctatagca gcaggtaaaa ctatagaaag tctggaaagt tttataggtt
    44881 cagataaaaa aatagtaaga gtaatgccta atactccagc ccttgttggc gaaggaatgt
    44941 cagctctttg taaaaatagt aatgtaacag atgaagagtt aaatatgatt aaggctctgt
    45001 ttgaatcttt tggtgaggca gaaatagtat cagaatattt aatggataca gttacaggta
    45061 ttagtggttc ttctcctgca tatgtattta tgtttataga agctatggca gatgcagcag
    45121 tattagctgg aatgccaaga caacaagctt ataaatttgc atctcaagca gtaatggggt
    45181 cagctaagat ggttcttgaa actggaaaac atccaggtga attaaaagat atggtttgtt
    45241 ctccagcagg aactactata gaagctgtta aggtattaga agaagaaggc tttagagcag
    45301 cagttataaa atctattgtt gcttgtatag ataagtctaa agatatgagc aaataacaga
    45361 tgtaattaag tttactatat ggtttcttga attgtttttt tagtgcttat ttatatctat
    45421 acataaatgt atttggtata agtaaaataa gagtaattta ctataaaaag gaatttaatg
    45481 attgagtgat a
//
LOCUS       DAMJYR010000019        45439 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.019, whole genome shotgun sequence.
ACCESSION   DAMJYR010000019 DAMJYR010000000
VERSION     DAMJYR010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 45439)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45439)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45439
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.019"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            49..888
                     /locus_tag="PC471_001009"
     CDS             49..888
                     /locus_tag="PC471_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010293327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDF2788148.1"
                     /translation="MKDYYKIGEISKIYGIGRDSLMYYEEIGILRPVRDINGYRMYNI
                     SDIWKLNLIKEFRSLNFPMKKIKEYLDDRSIESTKNILNEELDLIDKKIAEFISHKEN
                     IIKRLSSIESVIQNTKIDEIEVVYIEKRKALELNADIKRDEDFDFLIQKLQKEYEDRF
                     NILGNNNIGSAFSTEAINKGIFNEFKSVFCFLESNEKVYNIVFDEGYYVTLNYTGSNS
                     NNKMYMEKVFKFIEENNYKIIADPIEIYKIDIHETGIVEEFVTEIQVPITKYLYFNGL
                     DGK"
     gene            1003..1242
                     /locus_tag="PC471_001010"
     CDS             1003..1242
                     /locus_tag="PC471_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2788149.1"
                     /translation="MKCSNCGSVNIEKGILTTGGIYAETIGLKYRGDGKLDKVINKTF
                     PVQSTIYSDLCLDCGEIVRTYIKDNTDRNWCKDKK"
     gene            complement(1413..1589)
                     /locus_tag="PC471_001011"
     CDS             complement(1413..1589)
                     /locus_tag="PC471_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2788150.1"
                     /translation="MFRDEMDKCTHMLTAYISSLYDYCDFIDTQLDDFILEYGENVVE
                     SCLHQVMVLVSKYN"
     regulatory      complement(1643..1739)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            complement(2094..2768)
                     /locus_tag="PC471_001012"
     CDS             complement(2094..2768)
                     /locus_tag="PC471_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HDF2788151.1"
                     /translation="MNHHILIQFEYIARIITAGFCGALIGYERKNRLKEAGIRTHFIV
                     ALASALIIIVSKYGFNDIVGTPGIGLDPARVAAQVVSGIGFLGAGLIFIRNQSVSGLT
                     TAAGIWATAGTGLAIGSGLYLVGIVSSIIIVIVQITLHRDRKWMKLPTAEQVSLKISN
                     STDSIEYIEGKFKKSNIEIINMNIKRLDGDWLEVVIYTKLPKGYKKTNLLKLFSDNPL
                     IETLEI"
     gene            complement(2988..3542)
                     /locus_tag="PC471_001013"
     CDS             complement(2988..3542)
                     /locus_tag="PC471_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HDF2788152.1"
                     /translation="MSIALILIDIQNDYFKNGKCELFQSEETAENAKKILLFFRKNNL
                     PVIHIQHISTDKTASFFLPNTHGSEINKIVFPLDNEEIIIKHTPDSFFETDLQSILEK
                     KNITNLVICGMMSHMCIDTSVRTAKKLRYDITLISDACTTKNLSWNNIEINANTVHQV
                     FMASLQNSFADVKNTDKFLSNYYK"
     gene            complement(3620..3985)
                     /locus_tag="PC471_001014"
     CDS             complement(3620..3985)
                     /locus_tag="PC471_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2788153.1"
                     /translation="MKIRAEYTCPLEIVHDIIKGKWKTILIFELRQSNKTFSELQHNI
                     YGINQKMLIQQLKELRDFGIIDKISSAGYPLHVEYFLTNRGKKMLKAVEIMQEIGIEY
                     MLEHGQQEILDNKGICYNK"
     gene            4450..5235
                     /locus_tag="PC471_001015"
     CDS             4450..5235
                     /locus_tag="PC471_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDF2788154.1"
                     /translation="MIVKIDNTLEKEFWQYVSHEESLNLFIIGYVENYGFSSQYQDIW
                     SQVEGGNITSIILKNKSTLIIYSFKNNFNIGEMKNHIKDLDVESISGKKCVIDRLISK
                     YKDFYEKLDNKFCVLKEIKEIDFSNMKEYKIENAQEKDIDEIGKLLNRSDYKVSKNYI
                     EERKVHLKEGNVRAYFIRNDDTMISTVSTGMETSFLAMVVSVSTDKRYRGKGLASYMV
                     YNLSKELLLEGKVPCLFYNNDVAGKIYHNIGYKEINEWTILFK"
     gene            complement(5332..6156)
                     /gene="pdaA"
                     /locus_tag="PC471_001016"
     CDS             complement(5332..6156)
                     /gene="pdaA"
                     /locus_tag="PC471_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="delta-lactam-biosynthetic de-N-acetylase"
                     /protein_id="HDF2788155.1"
                     /translation="MKKDSLKKYIMIGAFALTLFGIASINFKSLDKTKTQISSPTLDT
                     HEYDWYFNPREDGKQPSPIKEADFFKKYGAYYVGNPNEKVIYLSFDAGYESGNTPKLL
                     DTLKKHNAKAQFFVVESYIKSNPELIKRMEKEGHLVCNHSKSHPSMAGITDFEKFKEE
                     ITSVEKAYKDVTGKEMPKYFRPPMGKFSEQSLKYTQDLGYKSIFWSFAYVDWYEKKQP
                     THEFAKNKIYSRTHPGAIVLLHPNSSTNTEILDEVLTHWEKEGYKLKTLDYLNNKK"
     gene            6377..9496
                     /locus_tag="PC471_001017"
     CDS             6377..9496
                     /locus_tag="PC471_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HDF2788156.1"
                     /translation="MDNIISVLNDVVFLDIEVSGLDCLNSEILEVGAVKVKDWKIYTY
                     ESLIKNKFEVPIEVFSVCKNLDKNDLEIANKIELVEDRLVNFVEDSFIICHDLSLKKK
                     FFEYHMPKLKNKFIDLIELAVILEPYHKDYSLEYLKNTLTNCNSKVENRALSDAIDII
                     NIVNCLLVKFNNYEKTTLEPLSFKINSYLKKFNLPTWEWSKFLEEANYDLSNNINIKK
                     EYNIFDSKEEKKKERETLKILNEEEKNYEELLKYKTIWENKEGFTYEYRPGQYELTKT
                     IRELFRNSEDEEKIACIEAPTGIGKSVGYLLPAILEARINKKRLLISTDTKELQIQLI
                     NKDIPNVLDSLGLNGKVSYGYIKGKNNYICIDRLEAYIDDYESQNPTKGEILSLIFLK
                     RLVEGGKYGDIEEINYLVFDNFKEISTHLRNVSCDPNMCRPKKCKKDCLYKNRIEELK
                     EEHITVVNHSLLAKWPYKDEKPLENIIVDEAHNLTEKGYDFFSSIINSKSLRYLLQEI
                     YPYEFIQNSSFIYKKYSRNMRKIKAFDKFYNVLKIGREDKQKIARSINFIIEEIDSIL
                     KFGNCNEYNNVSNYNLRWELNLQIDEIVGKLKKDGIDTEISYRAYSEKIKLSCEKIIK
                     NLVSIIIIIYRNIDDDSIDKEADIYKFGKAKTRDLEDIKIIFEIFLEYDEKDDYARIV
                     EIDKNYNDFEFRVVPLKIADLFEENILSQLEKGIFLSATLSLSESMSYFKNTLGIDRV
                     KNVEKIIEPIFDYKNRVSVVGFSDICEYRNSEFPNEMSKIISNISKITEGHTLALFNS
                     KDRQEKTYEILKKYLHSFNLEIYADKKGIRHLNDLNRKCVVLGSKGCFEGVDIPGDGL
                     VCVTLDKLPNLNPKDPLYFTIMKKYGIDYYTINYPQMTIKVKQAMGRILRSKYDYGCF
                     VIFDVGTNISVLKRLEKDLHDCKISKVNSNEFYTYIRRHLNKSRSLILKSVIFDTIKA
                     LNVDAKMDNNDVDKDIIKKDINENIRQRAVKGEVYHIDIIKKDMKVKYFDRNYLINLD
                     IFMREEDKN"
     gene            9514..10113
                     /locus_tag="PC471_001018"
     CDS             9514..10113
                     /locus_tag="PC471_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H family protein"
                     /protein_id="HDF2788157.1"
                     /translation="MAKQKFYAVKKGKNIGVYNTWDECKKQVNGFSGAEYKSFSTFQE
                     AKEYIDGSEKLSFQEDKEFIEAYVDGSYEHSVKMYGSGVVILKNNEVIKTYSEKGKEK
                     TLVSMRNVAGEIEASKIAMQYCIDNNVQNLILYFDYEGIEKWCTGVWKTNKEGTIAYK
                     NFYDSIKNKLNVKFTKVKAHSGNKYNEEADKLAKKAIGV"
     gene            complement(10340..12469)
                     /locus_tag="PC471_001019"
     CDS             complement(10340..12469)
                     /locus_tag="PC471_001019"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HDF2788158.1"
                     /translation="MPNLPSLGSKAPDFKANTTNGPIRLSDYKGNWIVLFSHPGDFTP
                     VCTTEFLCFAKYYDEFKKRNTELIGLSVDSNSSHLAWMYNISLLTGVEIPFPIIEDRD
                     MRIAKLYGMISKPMSDTSTVRSVFIIDNNQILRTILYYPLTTGRNIPEILRIVDALQT
                     SDRDNIVTPANWFPGMPVILPYPKNYKELKNRVNSCNKKYSCMDWYLCFVPDNYNDEE
                     VSKKIDNTCSWKKEHTKNIENECNCEHEHHDYLNKALDCKQEHKTDIKDDCNHEKKHT
                     KNTNKVHNSKQDKFKDKSCDEMNFNYDKDESCDKINSSYNKEDSSYEDFYKHNYKNYD
                     YTSEKNTKKIAMKTLKDSKKLVRPQITDPYNPIVENANCPDINPIVAEYVLGNPTNVD
                     AQLLDAVIFAFAEIDQSGNLFIPYPRFLNQLLALKGEKPSLKVIVAIGGWGAEGFSDA
                     ALTPTSRYNFARQVNQMINEYALDGIDIDWEYPGSSASGITSRPQDRENFTLLLTAIR
                     DVIGDDKWLSVAGTGDRGYINSSAEIDKIAPIIDYFNLMSYDFTAGETGPNGRKHQAN
                     LFDSDLSLPGYSVDAMVRNLENAGMPSEKILLGIPFYGRLGATITRTYDELRRDYINK
                     NGYEYRFDNTAQVPYLVKDGDFAMSYDDALSIFLKTQYVLRNCLGGVFSWTSTYDQAN
                     ILARTMSIGINDPEVLKEELEGIYGQF"
     gene            12735..12983
                     /locus_tag="PC471_001020"
     CDS             12735..12983
                     /locus_tag="PC471_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2788159.1"
                     /translation="MMICFAMTFALVLINSVMGVHIGLDYYFLMGSVLTVCLTIGIVL
                     CNARKIFDSKAIHKTRTKTVSNNKKQKSHSRDIKRKIS"
     gene            13149..14540
                     /locus_tag="PC471_001021"
     CDS             13149..14540
                     /locus_tag="PC471_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HDF2788160.1"
                     /translation="MNFRDLFEKAVDFIDEKRKSIVAISLSTLGVIALIIVFFLSSNE
                     FSVGNEANELLKIIEKRQYSIAVDYYTSIEKKFSDSKMERFNKSVSKKINKLLLNSGD
                     KYLDGDISQESFIGLINTVKELNKISIDVDDLLAQADRVREMYKEENTTYDIAINYIS
                     TVSILNGMGSNLDVYKQNIETIKESRDVYDSAVKDQKAYKYYEAIEKYNKVLKEDEKY
                     YSMAQNGKEQCIEEMYDYYISRAEEANTSGDYERALQYIEYLKDDYSDDEKVQSLEKK
                     YKKNLSLYTLTSDDIINVIAKKSGKDKANLSINSLPQMIKNNKYYYAEVYEYDKLINE
                     VLVSAKTKDLYSYKDGKKDYKVDYGDGYFRILEDGSYQFGITKDKAKFVLTNTLDEKE
                     NKYKKIEILDIEKADRYVKSKKSLEELFGKYKNIYYYAVVNKGLFRGKEVYAINIYNE
                     KIYAISENGLNEY"
     gene            14655..16124
                     /locus_tag="PC471_001022"
     CDS             14655..16124
                     /locus_tag="PC471_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HDF2788161.1"
                     /translation="MSNVNKKLVIAIGAVALVVIIFIGIMCMQFKGEKIAKNTYVNGV
                     NIGKLTKSQAKQELAKKYKLENVDFNYNDKSWKVKSKDLNLSTDLDKTVENAYNLNRK
                     SAFFGNLSKTISANFGKKSNLVVAINYDKNKLKAEMEKIAKEIDVDVKDATLDISGEK
                     VKVIPDSDGLKMDISKSMENFDNQTKKGNYKNELVVKATPAKVKKEQLANIDTNLGTY
                     STTFKTSQINRSINIKLATDNISNVLLMPGETFSFNKHTGKRSKENGYKSAPVIMEGE
                     MEEDYGGGVCQVSSTLYNSVLYAGLEIVNVKNHTIPSSYVPKGRDATVADSGIDFLFK
                     NNLKHPVYIKNYVSGNQIVCNIYGSAEDKQNITISTKLDGVSQTTMKRVNDPTMPKGK
                     EKVDKSGRNAYSVSTYRTFNDANGKKIKTEKIANSYYPKKEGIILVGTMEPKPEEKPN
                     TDENKNNQNTNNQNPNNQQKPETPPTDNKPNETQPQPQA"
     gene            16189..16782
                     /locus_tag="PC471_001023"
     CDS             16189..16782
                     /locus_tag="PC471_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fis family transcriptional regulator"
                     /protein_id="HDF2788162.1"
                     /translation="MNLNNITNYVQSICENISNVLDVDVTLVTKDLTRIAGTGIFRGK
                     IGETISEKSVYSLVLKDGKSYVINKEIEENCNKCANRENCKELADICTPVKMGEHILG
                     ILGIAAFNEEQKEKILSKDKELTAFVRSMSDLISFKLDELYKQEVKTIDDLEREAIEN
                     AIKKYGSNTEGMKKVAEALNIGIATLYRKVKKFNIKS"
     gene            16964..18310
                     /locus_tag="PC471_001024"
     CDS             16964..18310
                     /locus_tag="PC471_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HDF2788163.1"
                     /translation="MPRKILDISLIEDNKSDLTFFNIEEEDGDETLKALKQSYVTTNY
                     KEETLLNLQGALNSKSDTTSKELKLESYKRYNNALDLAYKNYITSAINMVNKALEINP
                     KDIDILNLRGLLKLLKCDFAKSFESFYTALCYENNHISRKYVNLISSEDFKTFLGRYN
                     HSIRFINEELNYESIHILENIIEEEPELIEPYVILSLLYDKLGNVKKREGYLDKLKEL
                     DKDNPVFEKNEEEKEDTSKKEEIKKVKRKKSILPYIIVGCVLIGMGIYLIQSKKRIEN
                     LNNQISSKEEKLSETDKKLGETSKKLEKTNKELNEVKNKEPEREITVADEEDLYYQAL
                     NLKKNKEYEKAIDNFKSVVSSGKTKKYISESIYQLAITNKLLGNKDEAIKYYKKYINT
                     YTKNDQYYDDSYYELGMLYYDNGDLKNAQQTFYSLRSEVPDSMYNNSKIKEILSEK"
     gene            18492..18863
                     /locus_tag="PC471_001025"
     CDS             18492..18863
                     /locus_tag="PC471_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HDF2788164.1"
                     /translation="MKVFNQLVIILSIWVIGEYISSFIQGIILIPGSIVGMLLLFLLL
                     QFKVLDLSSIENVSGFFLDNMAIFFIPAGVSLIKSLDLIRENVLVLLLVICLSTLIVM
                     YTTGIIVEKMIKKKEEGQKNV"
     gene            18856..19545
                     /locus_tag="PC471_001026"
     CDS             18856..19545
                     /locus_tag="PC471_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HDF2788165.1"
                     /translation="MYNVLQTPVFGIIVTIVFFNLGRYIQRKTSNPICNPLLIAIVGI
                     ILFLSISKIPYENYKIGADSLNFFLGPVTVVLAVPLYKQFELFKKHMFEIIVGIGCGI
                     VISFVSVLIIGKIANSDVSIINSLIPKSITTPMGISLANSLNGVESITVVAIIFTGIF
                     GGMVASTVFKLGNINHPVAKGIALGTSAHALGTTKAFELGEIEGAMSGVSIGVSGTIT
                     VILIPIIMNFI"
     gene            20065..20931
                     /locus_tag="PC471_001027"
     CDS             20065..20931
                     /locus_tag="PC471_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HDF2788166.1"
                     /translation="MSEVKNTLQKHPVLRYSTMLFGMLMTSIGINGFLRPAHLLSGGA
                     TGIATSINYLTNINVGLLTFLINIPIFILGFIYLEKEFCISSLVNMIVFSLLLGATQE
                     ISNIIPIHDILLQSVYGGILSGLGVGVVFRTRSSQGGTDIIAAILKIKKNIEMKDTAL
                     AINGLIVLTGSFLFGLDLALYTLIGLFLNAYSMSFIKDAMNYQKSVMVMSNEVDLIAE
                     DIMKSLVRGVTFLDAEGAYTHQKKKIIYTIVSSNEIPKIKDIALKYDKKAFISVNDVT
                     EVKGRGFKAKDL"
     gene            21173..21982
                     /locus_tag="PC471_001028"
     CDS             21173..21982
                     /locus_tag="PC471_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2788167.1"
                     /translation="MNLSKEYVNLYDNYLKYINEVNKEIRSIKNMKKRVLKRGDFAPE
                     NEVLELEGKTIRDIDDVDTKKPKNKIYKFSNGDVYVGKFLEGKMEGKGSYTFFVDEGT
                     VMEYIGEFKEDKKNGKGNFIFSNGNEYIGYFEEDMMSGIGNMLYSSKDEYIGTWKNGK
                     KDGKGIYKWSDGCIYAGEFKGGKMEGYGICYNSKGEVLYEGEWKNNLIHGKGTYIWEK
                     GKKYIGEFMHGKKHGQGTFYLNNELVYEGTWKFDKPSIFDRTLDEIFSLRL"
     gene            22126..23190
                     /locus_tag="PC471_001029"
     CDS             22126..23190
                     /locus_tag="PC471_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HDF2788168.1"
                     /translation="MKRLIALVLVVLIVLFYSPELLKEGYDLKEKFFQSEIWNEFLDK
                     ININDNSKKDKKEKSQNLQSNNTEGENLGESDIKNFEKISLGDNLSYVLSSIGKPGRI
                     DISEYGFDWYVYNQYGKEFAMVGLENDEVVALYSNSINSCENQDIKLNQDRQTVRTKI
                     TPLKYKRKGNTRYIINSENQYDIISKEGKYITIFYDIHEENRVCSYLIIDKSTEDEFK
                     NLYPDDSEELKKCFELEVIDLVNSVRNQRGLNSLRYSEQATLSSRKHSEDMRDNNFFD
                     HVNKKNETPFDRMKREGIVYTSAGENIAAGQINAIYAHEAWMNSEGHRKNILGNYNNI
                     GVGVIFGGSYKTYYTQNFYK"
     gene            23218..23937
                     /locus_tag="PC471_001030"
     CDS             23218..23937
                     /locus_tag="PC471_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HDF2788169.1"
                     /translation="MTSWKKKTVYKCLIAVVLFCGIVLISNFSKVSALMMDTNGNVLI
                     KHGSREKKLIAITFDDGPHPKETSQVLDVLKKYNVKATFFIAGKHAKWYKEPLVRASK
                     EGHEIGNHTFNHPDISNLSSSQIEEEIVKCEDILKEVTGKKPTLFRPPFGSYREKELI
                     EIAKKHDYKVVLWTGVDVKDWKNPGANSIADKIINKVQNGDIILLHDYATNDTVEALD
                     MFIPKMIEKGFKFVTVSELIK"
     gene            23961..24545
                     /locus_tag="PC471_001031"
     CDS             23961..24545
                     /locus_tag="PC471_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HDF2788170.1"
                     /translation="MILMIDNYDSFVYNLVQYIEELGETVVVKRNNEIKISDIEELNP
                     EVIVLSPGPCSPKEAGICIDIVEHFKGKKPILGICLGHQTIGHVFGGDIIKAQQPVHG
                     KVYSINHTNKGVFRGLKNPLNVTRYHSLIVDSNTVPKELEITAITDKGEIMGIRHKKY
                     LIEGVQFHPEAILSEYGHEMLKNFITEARERVHM"
     gene            24547..25896
                     /gene="pabB"
                     /locus_tag="PC471_001032"
     CDS             24547..25896
                     /gene="pabB"
                     /locus_tag="PC471_001032"
                     /EC_number="2.6.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component I"
                     /protein_id="HDF2788171.1"
                     /translation="MIREINTKLNSFEIFTIFRNEHDSFILDSAMDKEKLGRYSFISS
                     QPFKVLKYKDTDENPLEVLKEELHKYRVVNDTNLPFVGGAVGYLSYDLGNYIENLPRT
                     AVDDIEMPDMYFGFYNHVIVIDHLVQKTYIATPNIDIELEEKIIDDIEQRILKEEKKG
                     IDSICYEEKEVTPIRLKSNFTKEEFKNAVQSVREYIRQGDIYQANLTQRFSGETELTS
                     FELYRDLRRFSPAPFGAFLNFEDAHILSNSPERFIRCVNKRIETRPIKGTRPRGKDKE
                     EDLRLQQELRNSEKDRAELLMIVDLERNDIGRISKTGSVKVPELFVIEPYANVNHLVS
                     TVVGELKDDKDATDVIKATFPGGSITGAPKIRAMEIIDELEPTQRNVYTGSIGYIGFN
                     GDMDFNIAIRTIIKNDKKVYFQVGGGMTWDSDPDEEYQETLDKAKSIMKALRGYYEE"
     gene            25886..26629
                     /locus_tag="PC471_001033"
     CDS             25886..26629
                     /locus_tag="PC471_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HDF2788172.1"
                     /translation="MKNNVGFDSSLSKFGIGLFETIRVKNGIAVDLDIHIERMLSSIN
                     CLDLDIKYKKDFLINEIVTYIKKENVINKALRITVFDEGYNISIRDIPYNQETYEKGF
                     KLAISPIVRGNSLIHRHKTTNYFENIYTKNIANKTGYNDGIFLNSEGVILECSMSNIF
                     FVKGERVYTPSDRLPILNGIIKKRIIEICDELHIELIENEINISEISSFDFVFVTNSL
                     MGAIKVTEIDKIKFNKENVVFDKIVKLLE"
     gene            26663..27364
                     /locus_tag="PC471_001034"
     CDS             26663..27364
                     /locus_tag="PC471_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2788173.1"
                     /translation="MIGCIYEVYNEEITKIIENQNTLAVYLVGSSKDVDFTLYDVEIN
                     DIDVFVFVKEGEKQERILFKKKGIEFDVNYFSKDGVKKLLSNREYFFVKEMKDAKVLF
                     DRENISHIIKDISRNIYLEGPSKMSLEEKSFIKQDIGAKISNLKNKEKFDVFEYHFLT
                     NLYLKDIIIGYFNINDKWVPKDKKLLKVLKKENNELFELVSKVSENYDYQRLLDVYNY
                     IFKEIETKEVIKLIF"
     gene            27390..27962
                     /gene="folE"
                     /locus_tag="PC471_001035"
     CDS             27390..27962
                     /gene="folE"
                     /locus_tag="PC471_001035"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HDF2788174.1"
                     /translation="MNKVDKEKIQHAVREILEAIGEDPDREGLIETPNRVARMYEEIF
                     SGLSEEPRDHLKVLFADEKHEELVLVKDIPFYSCCEHHLVPFFGKAHIAYLPKGGRLT
                     GLSKLARVIDTLAKRPQLQERITKNAADIIMEELQPYGVLVVVEAEHMCMTMRGVKKP
                     GSKTVTSAVRGIFEKDIASRAEAMSLITMK"
     gene            28026..28817
                     /gene="folP"
                     /locus_tag="PC471_001036"
     CDS             28026..28817
                     /gene="folP"
                     /locus_tag="PC471_001036"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HDF2788175.1"
                     /translation="MFDYGKRTYIMGILNVTPDSFSDGGDFNNLDIAIQHAKDMVDQG
                     ADIIDLGGESTRPGHSYVDSDEELRRVIPVIKKLKQELDIPISIDTYKADVAEEALKL
                     GVTMVNDVWGLRKDKNMASVIGKYDAEVCIMHNQDGTNYDKDIMESIKDFFKVSIEMA
                     MSCGVKKEKIVLDPGVGFGKDFEQNIEVLRRLNELKDLGYPILLGTSRKSVIGKVLPV
                     EPKKRLEGTIATTVLGIRDGVDIVRVHDVYENLMAARMTDAIYRK"
     gene            28828..29193
                     /gene="folB"
                     /locus_tag="PC471_001037"
     CDS             28828..29193
                     /gene="folB"
                     /locus_tag="PC471_001037"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HDF2788176.1"
                     /translation="MDKILLSNLGFYGYHGVLKEENFLGQKFFVDMELYIDSREAGLS
                     DDINKSVSYAEVYNVVKDITENKQFNLLEALAENIAEEVLNKFILINGVMVRVRKPEA
                     PVNGIYDYFGVEIRRTRDE"
     gene            29186..29692
                     /gene="folK"
                     /locus_tag="PC471_001038"
     CDS             29186..29692
                     /gene="folK"
                     /locus_tag="PC471_001038"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HDF2788177.1"
                     /translation="MNKAYLGIGTNMGDRFDNLSRACELLKNSDSIYKVKESSLYETK
                     PWGYTEQADFLNMCVEIETEFEPYELLEYCQEIERELHRERIVHWGPRTIDVDVLFFN
                     DVVSTDERLTIPHPRIQDRAFVLIPLMDLNEELIINEKTIKEHLNLLSAEEREEVKEL
                     VGYERKPI"
     gene            29673..30458
                     /gene="aroF"
                     /locus_tag="PC471_001039"
     CDS             29673..30458
                     /gene="aroF"
                     /locus_tag="PC471_001039"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HDF2788178.1"
                     /translation="MKENLFNNNSKIEINTNNKKITVKKDKLIIAGPCAIESYEQLLE
                     TAKFVKSQGANILRGGAYKPRTSPNSFQGLKKEGLEILKAVKDEVGMAVITELMDVRD
                     MDELYSISDIIQIGSRNMQNFTLLSEVGKQNKPVMLKRGIASTITEWIGASEYIAIEG
                     NSNIIMCERGIRTYNDYTRNTLDLAAVPIIQKETGLPVVVDPSHATGVRYLVKPMSLA
                     SFACGADGIMVEVHPDPENALSDGAQSLCFNEFEDLMKSINNY"
     gene            30713..32503
                     /locus_tag="PC471_001040"
     CDS             30713..32503
                     /locus_tag="PC471_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HDF2788179.1"
                     /translation="MNDIKDIIEEIKARCDIASIISEYMNIKQSGANYKGLCPFHGEK
                     TPSFYINTSKQIYKCFGCGEGGDVINFVMKMENLDFMDAVKILANKCGIEINTNMNEE
                     TRIKIEKSKKFQDIHTEAARFYLSNLLGSKNLGYEYLRIRGLDDKIIKKFGLGFSLDS
                     WNSLMNTLISKGYKKQDLLECGLIAKNRDGTNCYDKFRNRVMFPIFDYRGNIIGFGGR
                     VLDDSLPKYLNSPDTLIFNKKQNLYGLNFARKNLESKTIVLVEGYMDLISLYQYGIKN
                     VVATLGTALTEQQGILIKRYADTAIISYDSDEAGIKATLRAIDILTKLGINVKVLDLK
                     DAKDPDEFVRKYGLSDYKKAMDVSTHYIKYKIDHLKKEFNIQKDEERVKFAKEASKII
                     KQLTSPVEIDFYTKYLSNQIDINVESIKREVYGKNYNKPYNNKNQKKIEEKVIEKVEV
                     RQDGKQLVEETLIKIMLEDKKIREIALLKVEESDFLLKESKEILNYMIKNQELDKITI
                     DKLKSLNISEEYLKELNSISLNSINLENTKEIEGIITNIRKNSLEEQINSLLREQQEL
                     ENNNDMKEVDGRVMEIALKIVEINKILKSL"
     gene            32521..33687
                     /gene="rpoD"
                     /locus_tag="PC471_001041"
     CDS             32521..33687
                     /gene="rpoD"
                     /locus_tag="PC471_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HDF2788180.1"
                     /translation="MENKSNKKELKKVTAKTLIEKGKKQGSLTLAEIMEAFSETELDK
                     DQVENLYETLGNLGIEITETKNYKADIDFSVADDDLSIGHLDEDAEAISHDDSSAIEI
                     ETVDLSLPKGISIDDPVRMYLKEIGKIPLLKPHEEVEFARRMHEGDEIAKQRLVEANL
                     RLVVSIAKRYVGRGMLFLDLIQEGNLGLIKAVEKFDYTKGYKFSTYATWWIRQAITRA
                     IADQARTIRIPVHMVETINKLIRVSRQLLQELGRDPKPEEIAKEMEMTEDKVREIMKI
                     AQDPVSLETPIGEEEDSHLGDFIPDDDAPAPAEAAAYSLLKEQIEDVLGSLNDREQKV
                     LKLRFGLEDGRARTLEEVGKEFDVTRERIRQIEAKALRKLRHPSRSKKLRDYLD"
     gene            33852..34541
                     /locus_tag="PC471_001042"
     CDS             33852..34541
                     /locus_tag="PC471_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HDF2788181.1"
                     /translation="MKLTDRLLKIASLVSDGKKIADIGTDHGYIPVYLLKEGKVPFAV
                     LADVNKGPLDNAHKEVIQNNLLDKVDLRLGSGIEILEIGEVEEVIIAGMGGILISELL
                     EAKKEVAHNVEKLILQPMQAQEELRYYLLNNGYEILEEVLVREDFRIYEIIVAKYTGK
                     NTIIEDEIYYEVGIKLLENKDSLFNDFIEKKIKTYSSIVNKLEGKNGEAIDKKRKESE
                     VAIKKLENLIK"
     gene            34555..35652
                     /locus_tag="PC471_001043"
     CDS             34555..35652
                     /locus_tag="PC471_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HDF2788182.1"
                     /translation="MLLKSLTRKIEKKYPLNLAEDWDNVGLIVGDFDMDVKKVLVSLE
                     ANEDVIDEAISKNIDLIVTHHPFIFGKINKINSGDLKGRLIQKLIKNDISLYSMHTNF
                     DIAFDGLNDYFMEIMEFGNSKVLDITKSENLYKLAVYVPHNYSDELRKVLSNSGAGHI
                     GNYSDCTFSTEGEGQFKPLEGSNPFLGSVNDIETVNEVKIETVVPQKLLGGVISSMLD
                     AHPYEEVAYDLYKLENKGEIFGLGRISKLDKSMTLESLSRKIKEKLNMKHIRVVGNLS
                     TDITKVAVVTGAGSEFVKKAKRQGAEVLITGDVKYHEAQDALDIGMCIVDCGHFDTED
                     IFKNVMKRFLDEFSEIEVIKSNVYLNPFSTI"
     gene            35715..36287
                     /locus_tag="PC471_001044"
     CDS             35715..36287
                     /locus_tag="PC471_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HDF2788183.1"
                     /translation="MKVAVIFHSVCGSTYLLAREYKEALEEMNIEVDIFRVSDEVAKT
                     LPQYYLINSKEYKDEFESINVIKSGKEILDYDAIFMGSPTYYGNVSGPMKMFMDSFSD
                     IWVGAPLSGKIFGCFATAGSQHGGGELALQAMNIFAQHMGMTLLSVPCSVRGGYPAYG
                     ILHIAGDNSDIRPNDDAKIGIRDYLKRLNI"
     gene            complement(36347..36814)
                     /locus_tag="PC471_001045"
     CDS             complement(36347..36814)
                     /locus_tag="PC471_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HDF2788184.1"
                     /translation="MFKEMRLKKREMTKEDTVEVLKNGEFGTFSTISENGYPYGVAVN
                     YVYFNDSIYFHCARNGHKLDNISKNNKVSFLVVANESVIPDKFSTTYSSAIVFGKACT
                     VENEEKKNALVEIIKKYSKGFFEEGMKYIEKDMNLTTVVKIEIDHISGKASRL"
     gene            complement(37200..37676)
                     /locus_tag="PC471_001046"
     CDS             complement(37200..37676)
                     /locus_tag="PC471_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HDF2788185.1"
                     /translation="MLSKAEIKYFNECASEILSSEKVQLMRTFPHHGNVSCLEHSLSV
                     AYYSYLLCKKLHLSVDIQSVIRGALLHDFFLYDWHYKGDRKGLHGFTHPREALKNATL
                     FFQINEKETDIILKHMWPLTVKPPRYKEAFIVCLLDKFCCLVETLKIHSLLSPYHV"
     gene            complement(37796..38887)
                     /locus_tag="PC471_001047"
     CDS             complement(37796..38887)
                     /locus_tag="PC471_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1648 domain-containing protein"
                     /protein_id="HDF2788186.1"
                     /translation="MSKIECLFTIFPVLILMYATFYFIPYFVGKKHIYGVSIDQEHKN
                     YNYFIKLDKEFKKLLSLGFIIDFILVFILVFTFNKLELSYFIGIIGFLLYESILYIHT
                     HKKAKKLKSEIYAKLGHIDIDSKLIVDMDFINKKNKIIKKFKIIYLIPILFTLGMSIF
                     IAFNYNQLPDSIATHWNINGSPDVFIDKSFLNVFKLIGTDFCLMVLLYITSIGSIKSR
                     IKIDTNRIEESRVENIKYLNKIGYLFLILMIIMTTQFFTTLLSIKTKLLTAMNITTLL
                     VIIYLIATYINSPNLKFNSSYSPEEDEKYWIAGIMYNNPNDPSLMVNKRFGIGWTINF
                     GNPLGKILYIAIALLLIFSLFSLIKSLLL"
     gene            complement(38880..39269)
                     /locus_tag="PC471_001048"
     CDS             complement(38880..39269)
                     /locus_tag="PC471_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDF2788187.1"
                     /translation="MVLNLDFNSDKSIYVQIKEEITKAIASGELKINESLPSVRSMAE
                     NIGVNLHTVNKSYNELKEEGFLNIDRRKGAIVAQLPMKIDNTTRQKNKLDLELLVAKS
                     YLSGISKEEFLSLSSSLFDKYEVKYYE"
     gene            39461..39907
                     /locus_tag="PC471_001049"
     CDS             39461..39907
                     /locus_tag="PC471_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2788188.1"
                     /translation="MLTYLMKRQMHKKDDGMSKPMMITGAVITGVGAYAVYKMVKSHK
                     SNSQMVNTNYYGNSDYDDYKYDEFDYDCDCRDTKQDYEYEELKKKVKEFNSRRINCEN
                     CPHVSQEEMMQYVNSVKDVKEELDLHEDAKKDNIYKEDEYKKYEEE"
     gene            40171..40896
                     /locus_tag="PC471_001050"
     CDS             40171..40896
                     /locus_tag="PC471_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2788189.1"
                     /translation="MKRKEIVMLKLLIVEDDSTISFGIKYALEQEGFSIDISKDLSSG
                     KEMISSNEYSMILLDVTLPDGTGYELCQYIRGFSQVPIIFLTACDEEVNIVMGLDIGG
                     DDYITKPFRIRELISRIKAVLRRKGNTSEENKKILKFGDLSIYTLEARVYKNDKEIFL
                     TSVEYKLLLILIQNKNTVLSRTQILEKLWDVTYDFVNDNTLTVYIKRLREKIGDDLCE
                     PIYIETVRGIGYKWIGSENSVSI"
     gene            40883..42127
                     /locus_tag="PC471_001051"
     CDS             40883..42127
                     /locus_tag="PC471_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF2788190.1"
                     /translation="MFLYNPEVKKFLSKYVTLMFIVIIISIGFSIINVSLTKEMVVRN
                     NQAIIGTLSSKYPNLESQIVDIITQGKSMENIDYGQKILIKYNYDKSIRINSEPIISK
                     LVLETIKINIILVCIIFILIFVLIVRYFTSIYNDLSDMTKYVYYSSEGKPFDMKNKNQ
                     EGQIGLLKTELLKMTTILNEKVELLKREKIFLNNTISDISHQLKTPMTSLIMLNDLLY
                     NDVPYEVKIDFLNKIKNQLNRMDWLIKSMLKLSKVEAKVINFKKDKVKFSELIHRAML
                     SMKIPMEIKNQKLTIEGNDNVSYIGDIDWSVEALVNIIKNCVEHTPEFGNIIITYKEN
                     PLFSELIIKDEGEGIDKKDIPHVFKRFYRGRSSSKEDSVGIGLAMSKSIIESQNGDIY
                     VNSEKGKGTEFHIIFHKMYDSD"
     gene            42207..42884
                     /locus_tag="PC471_001052"
     CDS             42207..42884
                     /locus_tag="PC471_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2788191.1"
                     /translation="MEILRVENLTKSYGKNETKVDAIKNVSLSVEKGTFIAITGPSGS
                     GKSTLLHLLGGVDRPTSGKVYINDVDIYNLKTKDLAIFRRRNIGLIYQFYNLIPVLNV
                     KENILLPAELDNRDIDREYFDDLMKTLGLIDREKHLPNQVSGGQQQRTSIGRALINRP
                     SIILADEPTGNLDSKNSKEILELLKLSVKKYNQTLIMITHDKNMALQADRIISIEDGI
                     IKSDEVM"
     gene            42885..>45439
                     /locus_tag="PC471_001053"
     CDS             42885..>45439
                     /locus_tag="PC471_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2788192.1"
                     /translation="MNLYTSLTIRYLKQNKRRTIVTIIGIILSTALICGIGNIFESFM
                     DYQMRETIKNDGSFHVTFYDVNRKNVEYVTKSAEIEKHAFTKQLGYSKLENSENAILS
                     IKQYDKNAINGYKVSIKEGRFPAKEGEIVLSESIINLIDDKLKIGDKITLKVGDMFDS
                     SKKKIDSMTFHEGDYIANEKDRTFKVVGIIEKPGFERYNGIATAIAYFNPMNNYNDTI
                     NVSVAVKNPKDVYKISNKISKNIYSNKDKEAVSDMIKYNEHLLRLQGASKYSNINSSI
                     KSIIIAVTILVIVCTIATVYNSFSISINERKKQFGILNSIGATSSQIKRLVFIEGIII
                     SLIGIPIGLISGTVAIDLLFKIINKYFTESVVTKMSLQIVYNPIIIIVSIIIVLLTIF
                     ISILLPAISASNISPLNVIKNTGEYKVGKIKSSRLIKMIFKTEGVLAYKNIRRNRKKF
                     IITLFSLMVSIIIFISFSGFTLLLLRGEEIRNSQRKYDLYLTAKNTARSVEDTISELE
                     DIDGIKNFELATGQYVSIRVSENKINKSKEDLIRKYYQKYKIGDSYEYDFSNNELIFP
                     GDFAVKNKINNLVQGSFNKERAIEENGVILVRKSAIEENGKKGVVELTNYKVGDTVNC
                     EYLDENALSKKVKVKILAITDEERLGLGYQNMGLQFITYDEVAKNLNLKLSKSLIFID
                     SGGSIKTKKEIEALANKNNFNFYDESASNESEKQDLKVIKIFVYGFIVVISLVSVTNI
                     LNTVSTSINLRKRELAVIQSIGVTPKGFRKMIYLESFIYGILSLLFGIPISIGIILIM
                     NKLISGVIEFSPIIPWTAIVICIVSVFIITFIAGYIPMSKLNKENIIDNIRRE"
BASE COUNT        18450 a         4636 c         7645 g        14708 t
ORIGIN      
        1 atgttgattt aaaataaaaa taagatttcg gaggagttta ttatataaat gaaagattac
       61 tataaaatag gagagatatc taaaatatat ggaataggta gagattcatt aatgtattat
      121 gaagaaatag gcattctaag accagttaga gatataaatg gatatagaat gtataatata
      181 tcagatatat ggaaattaaa tttaataaaa gaatttagaa gcttgaactt tccaatgaag
      241 aaaataaaag aatacctaga tgacagaagt atagagtcta cgaaaaatat tttaaatgaa
      301 gaactcgatt taattgataa aaaaatagct gaatttataa gccataagga aaatattata
      361 aaaagattga gttcaattga aagtgtaatt caaaatacaa aaatagatga gatagaggtt
      421 gtatatatag aaaaaagaaa agctttagaa ttaaatgctg atataaagag ggatgaagac
      481 ttcgattttt taattcaaaa acttcagaaa gaatatgaag atagatttaa tatacttgga
      541 aacaacaata ttggttctgc cttttctact gaagcaatta ataaaggcat atttaatgag
      601 tttaaatctg tattttgttt tctggaaagt aatgagaaag tatataatat agtttttgat
      661 gaaggatatt atgtaacttt aaattatact gggagtaatt ctaataataa gatgtatatg
      721 gagaaggtgt ttaaatttat agaagaaaat aattataaga taattgctga ccctatagaa
      781 atctataaga tagatataca tgagactgga attgtagagg agtttgtaac agaaattcaa
      841 gttcctataa ctaaatatct atatttcaat ggattagatg gtaaatagaa tgaaaaaatg
      901 ttgatttggc accgatttgc cactgtgtta taaaatcggt agcaaattat attttaaatt
      961 ataagatagc atgaaaaaat aattgtttag ggggttataa atgtgaaatg ttctaattgt
     1021 ggaagtgtaa atattgaaaa gggaatttta acaactggtg gcatatatgc tgaaactatt
     1081 ggattaaaat atagaggaga tggaaaatta gataaagtta taaataaaac ttttccagta
     1141 caatctacta tatattcaga tttatgttta gattgtggcg aaatagttag aacctatata
     1201 aaggataata ctgataggaa ttggtgtaaa gataagaaat agaataattt ttgaagttga
     1261 attttattaa agatacaagt gaaaaagtat aaatgggaag aaattgttat atggaagaaa
     1321 actttgcata tatggaagat tagtttttta atataataac atttttaaat gtagataaaa
     1381 ctaaaaaggg ttctctagaa aacaatttct atttaattat acttacttac caataccatc
     1441 acttgatgta aacaagattc tactacattt tctccgtact ctagtataaa atcatctagc
     1501 tgtgtatcta taaaatcaca ataatcatat aaactactaa tataagcagt taacatgtgt
     1561 gtacatttat ccatttcatc tctaaacata tatattacta tccccctaaa aaactttcat
     1621 aaaacaaaaa gaacaatatc tttttggcaa ctggctgaca taactcatag tttattttat
     1681 aagcgttagt ccctatagct ttgcgtcact aaatttctct agttttgccg atttagtttt
     1741 attctacaac taaaataata cactaggata agttattgtt caacatactt gtcagaaaat
     1801 tgaaatattt aacaatatct taacaatatg aaatctattt ttcactcaag aaacaaacta
     1861 tatgtataga atgtttaata aaaattatat ttagttttgt attgaagttt ttgtattaaa
     1921 aagtttagtt cacaagggtt acttggctag ataatagata aacaaaaata tcttgaaaaa
     1981 atatatatga atggcaaaaa acaagggatg aattttatat agactcatcc cttaattatt
     2041 tatatattga ccttgattaa atatattatt atttttaagc ttcaattttt atattatatt
     2101 tctaaagttt ctataagagg attatcgcta aataatttta ataaatttgt ttttttatat
     2161 cctttaggta acttagtata tataactact tctaaccaat ccccatcaag gcgtttaata
     2221 ttcatgttta taatttcaat attagatttt ttaaatttac cttcaatata ttctatggaa
     2281 tcagtgctgt tagaaatttt aagagaaact tgctctgctg tagggagttt catccatttc
     2341 ctatctctat gaagtgttat ctgtacaata acaataatga tacttgaaac aataccaact
     2401 aagtataatc cagagccaat ggcaagacct gtaccggctg ttgcccatat accagcagct
     2461 gtagtaagac cacttactga ttgatttcta ataaatatta atccagcacc taaaaaacct
     2521 ataccactga caacttgtgc tgcaacgcgg gctgggtcta gaccaatacc tggagtaccc
     2581 actatatcat taaatccata cttggaaaca atgattataa gtgctgaagc taatgccact
     2641 ataaaatgag tgcgtattcc agcttctttt agtctattct ttctctcata tccaattaaa
     2701 gccccacaga aaccagcagt aatgattctt gctatatatt caaattgaat caatatatga
     2761 tggttcattt atctagcacc cccttctata taatattata tatactataa tactacattt
     2821 tttatgtgaa tatagtaaag tttaaaattt atataatagt tatatatgaa tataaaatgt
     2881 ataaacgtat attatgtata taaaaatgat agaaagcatc taattttaaa tgattaaaga
     2941 gcaagcttaa ataaatcaaa cttgctcttt taacatagtg tatgttttta tttgtaatag
     3001 ttagataaaa atttatctgt gttttttaca tctgcaaaac tgttctgtag tgatgccatg
     3061 aatacttgat gtacagtatt tgcattaatt tctatattat tccaagataa attcttcgtt
     3121 gtacatgcat cgcttattag agttatgtcg tatcgaagtt tctttgctgt tcttacactt
     3181 gtatcaatac acatatggct catcattcca cagattacta gattagtaat attttttttc
     3241 tcaaggatac tttgcaaatc tgtttcaaag aaactatctg gtgtgtgttt aattataatt
     3301 tcttcattat ctagtggaaa aacaatttta tttatttctg aaccatgagt attaggaaga
     3361 aaaaaagaag ctgttttatc tgttgatata tgctgtatat gaataactgg taaattgttt
     3421 tttctaaaaa acagtagtat ttttttagca ttttcagcag tttcttctga ttgaaataat
     3481 tcacattttc catttttaaa ataatcattt tgaatatcaa tcaatattaa agctatagac
     3541 atgattatac ctcctattta tttttattat aatactattg taaaaaatgt aaattactta
     3601 ctttttggta tgtataggat tacttattgt agcaaattcc tttattatct agaatttctt
     3661 gttgaccatg ttcaagcata tattcgattc ctatttcttg cattatttcc actgctttta
     3721 acattttttt accacgatta gtaagaaaat attcaacatg taatggatat ccagcagaac
     3781 ttattttatc aataatccca aaatcacgta actcttttaa ttgttgaatc aacatttttt
     3841 gatttattcc ataaatatta tgttgcaatt cagaaaatgt tttattagat tgtcttaatt
     3901 caaatataag gatagttttc cattttcctt tgattatgtc atgtacaatt tcaagaggac
     3961 atgtatattc agctcttatt ttcatagcat atcaccccca gtaaaatgat aataaattat
     4021 atcacttgtc ttgtgatatg acaaatatat atgaactgta tataaatttt atgttttatt
     4081 aagttctaaa tttcttctat tgtagttcat atttagtaca taaataatta gtaagatata
     4141 agtaaataaa tatataacat gactatagaa atttaacttt aaaataactt atgataattt
     4201 agaggaggaa tatatatgaa tatatcaaga aaagctatga aaataataga attagcacaa
     4261 aaaattgcaa ataagagagg tatttcagta gaagaagctt ggagtgaagc agtaacagaa
     4321 tataaaaata aatacgaaca tattgcatag atatttaaaa atttagaata tttggtagaa
     4381 tataatgatt ttaaatgata ctataaatta taaggtatat cattattaac atagaaggag
     4441 gatatctgct tgattgtaaa aatagataat actttagaaa aagaattttg gcaatatgta
     4501 tcacatgaag aaagtttaaa tttattcata attggatacg ttgagaatta tggatttagc
     4561 tctcaatatc aagatatatg gtctcaggta gaaggtggaa atataacctc tataatatta
     4621 aaaaataagt caacacttat aatttatagt tttaaaaata attttaatat aggagaaatg
     4681 aagaatcata taaaagattt agatgtagaa agcataagtg gaaaaaaatg tgtaatagat
     4741 agactaataa gtaaatacaa agatttttat gaaaaactag ataataaatt ttgtgtatta
     4801 aaagaaataa aagaaataga tttttccaac atgaaggagt ataaaataga aaatgctcag
     4861 gaaaaagata ttgatgaaat aggaaaactt ttaaataggt cagactataa agtaagtaaa
     4921 aattacatag aagaaagaaa agttcattta aaagagggaa atgtaagagc atattttatt
     4981 agaaatgatg atactatgat tagcactgtg tcaactggta tggaaactag ttttttggca
     5041 atggtggtct ctgtaagtac agataaaaga tataggggaa aaggacttgc aagttatatg
     5101 gtttacaatt tgagtaaaga attgttgtta gaaggaaaag ttccatgtct tttttataat
     5161 aatgatgtag caggtaaaat atatcataat ataggataca aagagataaa tgaatggaca
     5221 attcttttta aatagctata aaaaataaaa aactctatcg agaattaaag tatttataaa
     5281 taactatatt tttactataa ttcatgatag agttttttta aatttatgat actatttttt
     5341 attatttaaa taatcaagtg ttttaagttt ataaccttct ttttcccaat gtgtaagaac
     5401 ctcatctaat atctcagtat ttgttgatga atttggatgc agtaaaacta ttgcaccagg
     5461 atgagttctt gaataaattt tgtttttggc aaattcatga gtaggttgtt tcttttcata
     5521 ccaatctaca taagcaaaac tccaaaatat tgatttatat cctaaatctt gtgtatattt
     5581 taaagattgt tcgctaaatt tacccattgg aggtctaaaa tacttaggca tttcttttcc
     5641 agtaacatct ttgtatgctt tttctacact tgtaatttct tctttgaatt tttcaaaatc
     5701 tgtaatacct gccattgatg gatggctttt tgaatgatta caaactaaat gtccttcttt
     5761 ttccatacgc tttattaact ctggattgct ttttatataa ctttcaacta cgaaaaattg
     5821 agctttagca ttgtgttttt taagcgtatc taataatttt ggagtgtttc cactttcata
     5881 tccagcatca aatgatagat atataacttt ttcattggga tttcctacat aataagctcc
     5941 gtatttttta aaaaaatcag cttcttttat aggtgatggc tgtttaccat cttccctagg
     6001 gttaaaatac caatcatatt catgtgtgtc gagtgttgga ctagaaattt gagtttttgt
     6061 tttatctaat gatttaaaat ttatactagc aattccaaaa agtgttagag caaatgctcc
     6121 tatcataatg tattttttta ggctatcttt tttcacaatt tcacctctta tttaaaattt
     6181 aaagtatatt tcttataata tagtttgaat atttatagtt aaaaatattc acatatacaa
     6241 ataataaatg aaagaagttt actgtaagat ataaagttga tattaattat cttaaataaa
     6301 gtgtatataa atgataaaat ataaagagaa atgattgtaa ataagttagg ctggtttaac
     6361 ttgaagggat ataaaaatgg ataatataat aagtgtattg aatgatgttg tttttttaga
     6421 tatagaagtt agtggattag attgtttaaa ttcagaaatt ttagaagttg gagctgtcaa
     6481 ggttaaagac tggaaaatat atacctatga atcattaata aaaaacaaat ttgaggtacc
     6541 tatagaagta ttctcagtat gtaaaaattt agataaaaat gatttagaaa tagctaataa
     6601 aatagaactg gtggaagata gacttgtaaa ttttgtagaa gatagtttca ttatatgtca
     6661 tgatttaagt ctgaaaaaaa agttttttga gtatcatatg ccaaagttaa aaaataaatt
     6721 tattgattta attgaattag cagttatttt agaaccatat cataaagatt atagcttaga
     6781 atacttaaaa aatactttaa caaattgtaa ttctaaagtg gaaaatagag ctttatcaga
     6841 tgctattgat ataataaata ttgtaaattg ccttttagta aaatttaata actatgaaaa
     6901 aactactttg gagccattga gttttaaaat aaattcatat cttaaaaaat ttaatcttcc
     6961 aacatgggaa tggagcaaat ttttagaaga ggcaaattat gatttatcaa ataatataaa
     7021 tataaaaaaa gaatataata tatttgactc aaaagaagaa aagaagaagg aaagagaaac
     7081 cttaaaaatt ttaaatgaag aagaaaaaaa ttatgaagaa ttattaaaat acaaaactat
     7141 ttgggagaat aaagaaggat ttacatatga atatagacct ggtcaatatg agcttactaa
     7201 aacaataaga gagttgttta gaaatagcga agatgaagaa aaaattgcat gtatagaggc
     7261 accaactggt ataggtaaaa gtgtaggata tttattgcct gcaatattag aggctagaat
     7321 taataaaaaa agattattaa tttctactga cacaaaggaa ttgcagatac aattaataaa
     7381 taaagatatt ccaaatgtgt tagattcttt gggtctaaat ggaaaagtaa gttacggata
     7441 tataaaggga aaaaataact atatttgtat agatagactg gaagcatata tagatgatta
     7501 tgagtctcaa aaccctacta aaggagaaat tttatcttta atatttttga agagattagt
     7561 tgaaggtggc aagtatggag atatagaaga aataaattat ttagtgtttg acaacttcaa
     7621 ggaaataagt actcacttaa gaaatgtaag ttgtgaccca aatatgtgca gacctaaaaa
     7681 atgcaaaaaa gattgtttat ataaaaacag aatagaagaa ttaaaagaag aacatataac
     7741 agtagtaaat cattcactat tagctaaatg gccgtataag gatgaaaaac ctttagaaaa
     7801 tataatagta gatgaagcac ataatttgac tgaaaaagga tatgatttct tttcaagtat
     7861 tataaattca aagtctttaa ggtacttatt acaagaaatt tatccttacg agtttattca
     7921 aaatagtagc tttatatata aaaaatatag tagaaatatg agaaaaataa aggcttttga
     7981 taaattttat aatgtattaa aaattggaag agaagataaa caaaaaattg ctagaagtat
     8041 aaatttcatt attgaagaga tagattcaat attaaagttt ggtaattgta atgaatataa
     8101 taatgtaagt aattataatt taagatggga actgaatttg caaatagatg aaattgtggg
     8161 aaaactaaaa aaagatggta tagatactga aataagttat agagcctata gtgaaaagat
     8221 aaagttgagt tgtgagaaaa taataaaaaa tttagtttca ataataataa taatttatag
     8281 gaatattgat gatgatagta tagataaaga agcagatata tataaatttg gaaaggcaaa
     8341 gacaagagat ttagaggata taaagattat atttgaaatc tttttagagt atgatgaaaa
     8401 ggatgattat gcaaggatag ttgaaattga taagaattat aatgactttg aatttcgagt
     8461 agtaccatta aaaatagcag atttatttga agaaaatatt ttatctcaat tagaaaaagg
     8521 aatattttta tcagcaactt tgagtttatc tgaaagtatg tcttatttta aaaatacatt
     8581 aggaatagac agagttaaga atgtggaaaa gattatagaa ccaatttttg attataaaaa
     8641 tagagtctct gtagttggat tttctgatat ttgtgagtac agaaattctg aatttccaaa
     8701 tgagatgagt aaaattatat ctaatatatc aaagattaca gaaggtcata cattagctct
     8761 ttttaatagt aaagatagac aagaaaaaac ttatgaaata ttgaaaaaat acttacatag
     8821 ttttaactta gaaatttatg ctgataaaaa gggcattaga caccttaacg atttaaatag
     8881 aaaatgtgta gtacttggct caaaaggatg ttttgaaggc gtagacattc caggggatgg
     8941 tttggtttgt gtaactttag acaagcttcc gaatctaaat cctaaagacc ctttgtattt
     9001 tactataatg aagaagtatg gtatagacta ctatactata aattatcctc aaatgaccat
     9061 aaaggtaaag caagctatgg ggagaatttt aagaagcaaa tatgactatg gatgttttgt
     9121 gatttttgat gtaggaacta atataagtgt gttaaagaga ttagagaaag atttacacga
     9181 ttgtaaaatt tcaaaggtta atagcaatga gttttatact tatataaggc gacatttaaa
     9241 taaatctaga agtttaatac taaaatctgt tatatttgat actataaaag cgttgaatgt
     9301 tgatgctaaa atggataaca atgatgttga taaggatatt ataaaaaaag atataaatga
     9361 aaatataaga caaagagctg taaaaggaga agtgtaccat attgatatta taaaaaaaga
     9421 tatgaaagta aaatattttg atagaaacta tcttataaat ttagatatat ttatgagaga
     9481 agaagataaa aattaagagt gggagtgata tatttggcaa aacaaaagtt ttatgctgtg
     9541 aaaaaaggta aaaatatagg tgtatataat acatgggatg agtgcaaaaa acaagtaaat
     9601 ggattctcag gagctgaata taagagtttt tctacttttc aagaagctaa agaatatatt
     9661 gatgggtcag aaaaattgag ttttcaagaa gataaggagt ttatagaagc atatgtagat
     9721 ggaagttatg aacattcagt aaaaatgtat ggttcgggtg ttgtgatttt aaaaaataat
     9781 gaagttataa aaacttatag tgaaaaagga aaagagaaaa ctttagtaag tatgagaaat
     9841 gtagctggag agatagaggc ttctaaaatt gcaatgcagt actgtattga caataatgtt
     9901 caaaacttga ttttatactt tgactatgaa gggattgaaa agtggtgtac tggagtttgg
     9961 aaaacaaata aagaaggaac tatagcatat aaaaatttct atgacagtat taagaataag
    10021 cttaatgtaa aatttacaaa ggttaaagct cattctggaa ataagtataa tgaggaagct
    10081 gataaattag ctaaaaaagc tattggtgta taataaaaaa atatttatac ataattacat
    10141 ggtataagag tatatctaaa ttcctacttt ataattaaac aacttgtaat taaataaatt
    10201 ttgattaaaa aagcagattg tattatgaaa aaataagtat ctaatttata aacaattaat
    10261 aatagtatta attagataca acttatttaa tataataaaa tctgcttttt atataactat
    10321 aaaattttac tacattctat tagaattgcc cataaatacc ttcaagttct tcttttaata
    10381 cttcagggtc atttatacca atagacatgg ttctagccag tatatttgct tggtcataag
    10441 ttgatgtcca tgagaataca ccacctagac aatttctaag aacatattga gtttttaaga
    10501 atattgataa agcatcatca tatgacattg caaaatctcc atccttaact aaatacggaa
    10561 cttgagcagt attatcaaat ctatattcat atccattttt atttatataa tcccttctaa
    10621 gctcatcata agttcttgtt atagtagcac ctaatcttcc ataaaatggt ataccaagaa
    10681 ggattttttc agaaggcatt ccagcattct caagatttct taccattgca tcaacactat
    10741 atcctggcaa agataagtct gaatcaaaaa gatttgcttg atgttttcta ccatttgggc
    10801 ctgtttcacc tgctgtaaaa tcataactca taagattaaa ataatctatt ataggagcta
    10861 ttttatctat ttcagcactt gaatttatat atcctctatc tcctgttcca gctacactaa
    10921 gccatttatc atctcctata acatctctta tggcagttag taagagtgta aagttttctc
    10981 tatcttgagg tcttgatgtt attccagatg cactacttcc aggatattcc cagtctatat
    11041 ctattccatc taaagcatat tcatttatca tctgattgac ctgtcttgca aaattatatc
    11101 tagatgtagg tgttaatgct gcatcagaga atccttcagc tccccaacct ccaatagcta
    11161 caattacttt taagctaggt ttttcacctt taagagcaag taattgattt aaaaatctag
    11221 gataaggaat aaacaaattt ccagactggt ctatctcagc aaaagcaaat ataactgcat
    11281 ctaatagttg agcatctaca ttagttggat ttccaagaac atattctgct acaattggat
    11341 ttatatctgg acagtttgca ttttcaacta ttggattgta tgggtctgtt atttgtggtc
    11401 taactaattt tttagaatct tttaaagttt tcatagctat ttttttagta tttttttcgc
    11461 tagtataatc gtagttttta taattatgtt tatagaaatc ttcataacta ctatcttctt
    11521 tgttatagct agaatttatt ttgtcacaag attcatcttt atcatagtta aaattcattt
    11581 catcacaaga cttatcttta aacttatctt gtttggagtt gtgaactttg ttagtatttt
    11641 tagtatgttt tttctcatga ttgcaatcat ctttaatatc agtcttgtgt tcttgtttac
    11701 agtcaagagc tttgttcagg tagtcatgat gttcatgttc acagttacat tcattttcaa
    11761 tgtttttagt atgttctttt ttccagctac aagtattatc aattttcttg ctcacttctt
    11821 catcattata attatctggt acaaaacata agtaccagtc catacatgaa tatttcttat
    11881 tacaactgtt aactctattt tttaattcct tatagttttt aggataaggt aaaatcactg
    11941 gcattccagg aaaccagttt gcaggagtaa ctatattatc tctatcacta gtctgaagtg
    12001 catctactat tctaagtatt tctggaatat ttcttcctgt agttagtgga taataaagaa
    12061 tcgttcttag aatttgatta ttatctataa taaatacaga gcgaacagtt gatgtatcac
    12121 tcattggttt tgatatcatg ccgtataact tggcaattct catatctcta tcttctataa
    12181 taggaaatgg aatttctaca cctgtaagta aagaaatatt atacatccaa gctaaatgtg
    12241 aactgttact atcaacactt agaccaatta gttctgtatt tcttttttta aattcgtcat
    12301 aatatttagc aaaacataaa aattctgtag tacaaactgg tgtaaaatca ccaggatgtg
    12361 aaaataaaac aatccaatta cccttatagt cagagagtct aataggacca tttgttgtat
    12421 tggctttaaa atcaggagcc tttgacccta aacttggcaa atttggcatg taaatcaccc
    12481 ctcaataaat tatcatttat tttattctat taattaatac atattagtgt tactatttta
    12541 ttaaaagtta agtaatcctt atgtcaaaaa aagatattta ttaaaaagat ttaatatttt
    12601 cctaatataa atgacaatag taattgctat ttttttacta atttggtata gtaatggata
    12661 aggggtagtg tcaattttag tgattatacg aggtgagtgg attgaaaaaa attctaagaa
    12721 ttttagttat ctccatgatg atatgttttg caatgacttt tgcattagtg ttaataaatt
    12781 cagttatggg agtacatata ggattggatt attacttttt gatgggaagt gtgcttacag
    12841 tttgtttaac aattggaatt gttttatgca atgctagaaa aatatttgat agcaaagcta
    12901 tacacaagac aagaacgaaa actgtcagta ataataaaaa acaaaagagt cattctaggg
    12961 atataaaaag aaagatatca taatttatgt aaaggaaata tttatgtata aaataatgat
    13021 tgataggtaa aataaaaaca cgatttaact tgtgttttta ttttttttca agaattatga
    13081 tataataacc aataagaaat ttagtttacc ataaaatata aagattatta aatggagagg
    13141 gaactataat gaattttaga gatttatttg agaaggctgt agattttatt gatgaaaaaa
    13201 ggaaatctat agttgcgatt tcattatcaa cattaggtgt aattgctttg ataatagtat
    13261 ttttcttaag tagtaacgag tttagtgtag gaaatgaagc taatgagttg ttaaagatta
    13321 tagagaaaag acagtattct atagctgttg attactacac aagtatagaa aaaaagtttt
    13381 ctgattcaaa aatggaaagg tttaataagt ctgtttctaa aaaaataaat aaattgctat
    13441 taaatagtgg tgataaatat ttagatggag acataagtca agaaagtttt attggattaa
    13501 taaataccgt gaaagaatta aacaaaataa gtatagatgt ggatgactta ttagctcaag
    13561 ctgatagagt tagagaaatg tataaagaag aaaatactac ttatgatatt gccattaact
    13621 acataagtac tgtttcaata ttaaatggta tgggtagtaa cctagatgta tacaaacaga
    13681 atatagaaac aatcaaagag tctagagatg tttatgactc agcagtaaaa gaccaaaaag
    13741 catataagta ttatgaagct attgaaaagt ataataaagt attaaaagaa gatgaaaagt
    13801 attacagtat ggctcaaaat gggaaagaac aatgtattga agaaatgtat gattactata
    13861 taagtagagc tgaagaggca aatacttctg gagattatga aagagcatta caatatattg
    13921 agtatttaaa agacgattat tcagatgatg aaaaggtaca gagtttagaa aaaaaatata
    13981 aaaagaattt atcattgtat actttaacat ctgatgacat tataaatgta atagctaaaa
    14041 aaagtggcaa agacaaggca aatctatcta taaattcact tccacaaatg ataaaaaata
    14101 ataaatacta ttatgctgaa gtatatgagt atgataaatt aataaatgaa gttttggtaa
    14161 gtgcaaaaac taaagattta tattcatata aagatggtaa aaaagattac aaagtagatt
    14221 atggtgatgg atactttaga atattagaag atggaagtta tcaatttggt attacaaaag
    14281 ataaagctaa gtttgtgtta actaatactc ttgatgaaaa agaaaataag tataaaaaga
    14341 ttgagatatt agatatagag aaagcagata gatatgtaaa aagtaaaaag agtttagaag
    14401 aattgtttgg aaaatataaa aatatttatt attatgctgt agtaaataaa ggcttattta
    14461 gaggcaaaga agtttatgct attaatattt acaatgaaaa aatatatgcc atatcagaaa
    14521 atggactaaa tgaatattaa ttaataatat taaattaata tataattaaa aagtttttgg
    14581 ggttttaagg cgcattatgt agtaatataa taatagggat atataaataa ttttattgta
    14641 aggagaagta tgggatgagt aatgtgaaca aaaagctagt tatagctatt ggagctgtag
    14701 cattagtagt aataattttt ataggcataa tgtgcatgca atttaaaggg gagaaaatag
    14761 caaaaaacac atatgttaat ggagtaaata ttggaaaatt aactaaatca caagcaaaac
    14821 aggaattagc taaaaaatac aaattagaga atgttgattt taattacaat gataaaagct
    14881 ggaaagtaaa gagcaaagat ttaaatttat ctactgacct ggataaaaca gtagaaaacg
    14941 catataattt aaatagaaag agtgctttct ttggcaatct atcaaaaact atttcagcaa
    15001 attttggcaa aaaaagcaac ttagtagttg cgataaatta tgataaaaat aaattaaaag
    15061 cagaaatgga aaagatagct aaagaaatag atgtagatgt aaaagatgct acacttgata
    15121 taagtggtga aaaagtcaag gttataccag attctgatgg tcttaaaatg gatatttcta
    15181 agagtatgga gaattttgat aatcaaacta aaaaaggtaa ctataaaaat gagttagtag
    15241 taaaagctac tccagcaaaa gtaaaaaaag agcaacttgc taatatagat acaaatttag
    15301 gtacgtattc aacaacattt aaaacttctc aaataaatag aagtataaac ataaaattag
    15361 caacagataa tataagtaat gtattactta tgccaggaga aactttctca tttaataaac
    15421 atacagggaa aagaagtaag gaaaatggat ataaaagtgc acctgtaata atggaaggtg
    15481 agatggaaga agattatggt ggaggagttt gtcaagtttc atctacgtta tacaattctg
    15541 tgctatacgc aggtttagaa attgttaatg taaaaaatca tactatacca tcaagttatg
    15601 ttccaaaagg aagagatgca actgttgcag atagtggaat agatttctta tttaaaaata
    15661 atctaaagca tccagtttac ataaaaaatt acgtttcagg aaatcaaatt gtttgcaata
    15721 tatatggaag tgcagaagat aaacaaaata taacaatatc aacaaaatta gatggcgttt
    15781 cacaaactac aatgaaaaga gttaatgacc ctacaatgcc taaaggtaaa gaaaaagtag
    15841 acaaaagtgg aagaaatgca tattctgtat caacatatag aacatttaat gatgcaaatg
    15901 gtaaaaaaat taaaactgaa aaaatagcta attcatatta tcctaagaag gaaggtatta
    15961 tattagttgg gactatggaa ccaaaaccag aggaaaaacc aaatacagac gaaaataaga
    16021 ataatcaaaa tacaaacaat caaaacccaa ataatcaaca aaaaccagaa acaccaccaa
    16081 ctgataataa gcctaatgag acacaaccac aaccgcaagc ttaaagttct caagaatagg
    16141 atttatgtag agtaataaaa aatataaatc taaataggag ggattacttt gaatttaaat
    16201 aatataacga attacgttca aagtatttgc gagaatattt ctaatgtctt ggatgtagat
    16261 gttactttag ttacaaaaga tttaactagg atagcaggta caggaatatt cagaggtaaa
    16321 ataggagaga caatatcaga gaagtcagtg tactctcttg ttttaaaaga tgggaaatcc
    16381 tatgttataa ataaagaaat agaagagaac tgtaataaat gtgcaaatag agaaaattgt
    16441 aaagagttag ctgatatatg tactcccgtt aaaatgggag agcatatact tggaatcttg
    16501 ggtatagctg cgtttaatga agaacaaaaa gaaaagatac tttcaaaaga taaggagtta
    16561 actgcttttg taaggagtat gtctgattta atatctttta aactagatga actttataaa
    16621 caagaagtta agactataga tgaccttgag agggaagcta tagaaaatgc cataaagaaa
    16681 tatggaagta atacagaagg tatgaaaaaa gtagctgagg cattaaatat aggtatagct
    16741 acactctata gaaaagtaaa aaaattcaac attaaaagtt aatagtattg aaaaagaggt
    16801 aaccttttta tgaaaagaag aggattacct tttttattat gagaatggtg gaaaaatatg
    16861 tatagttttg acttgacata ttaaatatgt tatcttaaat atatatttta gagtggaggt
    16921 gagtattatt ttagttaaag atataaaaca agacatagaa agcatgccta gaaaaatttt
    16981 agatatatct ttgatagaag ataataaaag tgatttgact ttttttaata tagaagaaga
    17041 agacggtgat gaaacattaa aagcattaaa acagagttat gtgactacaa attataaaga
    17101 agaaactcta ttaaacttgc aaggagcttt aaattcaaag tcagatacaa cgtctaagga
    17161 gttaaaatta gaatcatata aaagatataa caatgcattg gatttagcat ataaaaatta
    17221 tataactagt gccattaata tggttaataa agctctggag ataaatccta aagacattga
    17281 tatactaaat cttagaggtc ttttaaaatt attaaaatgt gattttgcta aatcatttga
    17341 gagtttttat actgctttat gttatgaaaa taatcatata tctagaaaat atgtaaatct
    17401 aatttcatct gaagatttta aaactttttt aggaagatac aatcattcta taagatttat
    17461 aaatgaagaa ttaaattatg agtcaataca tattttagag aacataattg aagaagaacc
    17521 tgaactaata gagccatatg taattttatc tttgttatat gataaactag gtaatgtaaa
    17581 aaagagagaa ggatatttag acaaacttaa agagctagat aaagataatc ctgtatttga
    17641 aaaaaatgaa gaagaaaaag aagatacaag taaaaaggaa gaaataaaaa aagtaaaaag
    17701 aaagaaaagt attttgccat atattatagt tggctgtgtt ttaataggta tggggatata
    17761 tttgattcaa agcaaaaaga gaatagaaaa cttgaataac caaataagta gtaaagagga
    17821 aaagttaagt gaaactgata aaaagttagg agaaacgagt aaaaaattag aaaaaacgaa
    17881 taaggaatta aatgaggtta aaaataaaga accagaaaga gagataactg tagctgatga
    17941 ggaagattta tattatcaag ctctaaattt aaagaaaaac aaagaatatg aaaaagccat
    18001 agataatttt aaaagtgtag tttcttctgg taaaactaaa aaatatattt ctgagtcaat
    18061 atatcaatta gcaataacaa ataaattatt aggaaataag gatgaagcta taaaatatta
    18121 caaaaaatat ataaatactt atactaaaaa tgaccagtat tatgatgatt catattacga
    18181 gttgggtatg ctttattatg ataatggcga tttaaaaaat gcacaacaga ctttttatag
    18241 tttgagaagt gaagttccag acagtatgta taacaattca aaaataaaag aaattttaag
    18301 tgaaaaatag gtttatatag ttaagttttt tgaaacaacc aagtatgtat tttattaaat
    18361 taaaataaaa ataaatttaa aaagagattg tgtcaaatta taactttcta ttttaaagaa
    18421 agatatttgc ttgttgataa aactacaaat atatgatttg aagcaatctc tttaattttt
    18481 ggaggtggaa aatgaaagtt ttcaatcaat tggtaataat tttatctatc tgggtaatag
    18541 gagagtatat aagttccttt atacaaggaa ttatacttat tcctgggagt atagttggta
    18601 tgctattatt atttctacta ctacaattta aagttttaga cttgagtagt atagaaaatg
    18661 taagtggatt tttcctcgat aatatggcta tattttttat tccagcaggt gtatcactta
    18721 taaagtcatt agacctaata agggaaaatg tattagtgct tttattagtc atatgtttaa
    18781 gtacactaat agtaatgtat actactggaa ttattgtgga gaaaatgata aaaaagaagg
    18841 aagaaggaca aaaaaatgta taatgtatta cagacaccag tatttggtat aatagtaaca
    18901 atagtttttt tcaatttagg aagatacatt caacgaaaaa caagtaatcc tatatgtaac
    18961 cctttattaa ttgcaatcgt aggaataata ttgtttttaa gcatatctaa aatcccttat
    19021 gagaattaca agataggagc tgatagtcta aatttctttt taggtcctgt cacagtagta
    19081 cttgcagttc cactttataa acaatttgaa ttatttaaaa agcatatgtt tgagattatt
    19141 gtaggaatag gttgtggtat agtgatttct tttgtatctg tattgataat tggcaaaata
    19201 gctaattcag atgtaagtat aataaattca ctaattccaa aatctataac aacaccaatg
    19261 ggaatatctt tagcaaattc tttaaatggt gtagaatcaa taacagttgt agccataatt
    19321 tttacaggta tttttggtgg tatggttgct tctacagtat tcaaactggg gaatataaat
    19381 catccagttg ctaaaggaat tgctcttgga acatctgcac atgctttagg aactacaaag
    19441 gcttttgaac ttggagaaat agaaggagct atgagtggtg tttctattgg agtctctggt
    19501 acaattacag taatactaat tccaattata atgaatttta tataaaaaat aaaattaaga
    19561 cgagtaactt gaatacttaa aacaaaataa aataaaaaaa atttaaaata aaaactgaac
    19621 gaaaaaaatg caaataatga aataaaaaaa tcatgtactg aaattataaa aagaaaaatt
    19681 tcatttagga attaattata aagagacaat tactaaactt gaatttgcta aaataaaata
    19741 aattactgcg aaaatagata tttagaaaaa aatactataa atttactata ttgcaagtta
    19801 aataataagc agtatcatat tatgctagaa aaagtttcat ttaaggcata aatgagatga
    19861 tttgaacgtt tttaaagatt agaatataaa agattgcact agacatatat gaaaaagata
    19921 atataataga tgatgtaata gatgacagtt caagaaatta taaaaaaaat ttatttgaaa
    19981 tattatctat tacatcagga ataatatatg taagagtaag cttttaaaat tctaaagatt
    20041 tagaaagatt gaagggagaa agaaatgagt gaagttaaga ataccctaca aaaacatcca
    20101 gttttaagat attcaacaat gctatttggg atgttgatga catctatagg aataaatggg
    20161 ttcttaagac cagcacatct acttagtgga ggtgctacag gtatagcaac atctataaac
    20221 tatttgacaa atataaatgt aggtttatta acatttttaa tcaatattcc aatatttatc
    20281 ttagggttca tataccttga aaaagagttt tgtatttcaa gtttggtaaa tatgattgta
    20341 ttttcattat tattaggagc aactcaggaa ataagtaata ttatacctat acatgatata
    20401 ttgcttcaaa gtgtatatgg tggtatatta tctggtttag gagtcggagt tgtatttaga
    20461 actaggtctt ctcaaggtgg tacagatata atagctgcaa tactaaaaat taagaagaat
    20521 atagaaatga aagacactgc tttagcaata aatggcttga tagtacttac aggaagtttt
    20581 ttatttgggt tggatttagc attatacact cttataggtc tatttctaaa tgcgtattca
    20641 atgagtttta ttaaagatgc aatgaattac caaaaatctg ttatggtaat gtcaaatgaa
    20701 gtcgatttaa tagcagaaga tataatgaag agtttagtta gaggtgtaac ttttttagat
    20761 gcagaaggtg catacactca tcaaaaaaag aagattatat atacaatagt ttcatcaaat
    20821 gagataccaa agataaaaga tatagctttg aagtatgata aaaaggcctt tatatctgta
    20881 aatgatgtta cagaagtaaa aggaagaggg tttaaagcaa aagatttata gaaaatttta
    20941 caagctggga gtttataatg taaatttgta tattatatct ctcagcttat ttaaatttag
    21001 tatacgaaaa taaaggtttt attaatgatt tgtgtatcac tttaaaagat atcattttaa
    21061 aattaaaatt gaaaaaatta cttaatatgt agaaaacatg accactatta ggatataata
    21121 aatttaatag atttagtaaa atatctagga ataacgttgg aggaatggca ttatgaattt
    21181 atctaaagaa tatgttaatt tgtatgataa ttatttgaaa tatataaatg aagtaaacaa
    21241 agagattaga agtataaaga acatgaagaa acgtgtttta aaaaggggcg attttgctcc
    21301 agaaaatgaa gtattagaat tggaaggaaa aacaatacgc gatatagatg atgtcgatac
    21361 aaaaaaacca aaaaataaaa tctataaatt ttcaaatggg gatgtatatg tagggaaatt
    21421 cttagaggga aagatggagg ggaaaggctc atatactttt tttgtagatg aaggaacagt
    21481 tatggagtac ataggagaat ttaaagaaga taaaaaaaat ggaaaaggaa actttatatt
    21541 ttcaaatgga aatgaatata taggttattt tgaggaagat atgatgagcg gtattggaaa
    21601 tatgttatat agtagtaaag atgaatatat tggaacttgg aaaaacggaa aaaaagatgg
    21661 aaaaggtatt tataagtgga gtgatggatg tatatatgca ggtgaattta aaggtggaaa
    21721 gatggaaggg tacggtattt gctataatag taaaggggaa gtactttatg aaggagaatg
    21781 gaagaataat ttgattcatg gtaagggaac ttatatatgg gaaaaaggta aaaagtatat
    21841 aggtgaattt atgcatggta aaaaacatgg gcaaggaact ttttatctaa ataatgaatt
    21901 ggtttatgaa gggacttgga aatttgataa gccatctata tttgatagaa cattagatga
    21961 aatattttcc ttgagactgt agctttatct aattagtaga taaagctttt ttctaatact
    22021 tactgtaaat aggtatgaat gatattgatt agtatacact atcaatatta acttaataaa
    22081 tgcaatatta aaaaattttg attgatttta agaaggagag atatattgaa gagattaatt
    22141 gctttagtgt tagtagtact tattgtttta ttttatagtc ctgaattatt aaaagaagga
    22201 tatgatttaa aagaaaagtt ttttcaaagt gaaatatgga atgaattttt agacaaaata
    22261 aatataaatg ataattctaa gaaagataaa aaagaaaaaa gtcaaaattt acaatcaaat
    22321 aatactgaag gggagaactt aggagagtca gatataaaga attttgaaaa aataagctta
    22381 ggagataatt taagctatgt tttatcaagt atcggcaaac ctggtagaat tgatattagt
    22441 gaatatggtt ttgattggta tgtatataat caatatggaa aagaatttgc aatggttgga
    22501 cttgaaaatg atgaagtagt agcactttat agtaattcta ttaattcatg tgaaaatcaa
    22561 gatataaaac taaatcaaga tagacaaaca gtgagaacta agattacacc attaaaatat
    22621 aagagaaaag gaaataccag atatataata aactcagaaa atcaatatga tataataagt
    22681 aaggaaggta agtatattac aattttctac gatattcatg aagaaaatag agtttgttca
    22741 tatttaataa ttgataaaag cactgaagat gagtttaaaa atttatatcc agatgatagc
    22801 gaagaactta aaaaatgttt tgaacttgaa gttatagacc ttgtaaatag tgttagaaat
    22861 caaagagggt taaactcatt gagatacagt gaacaggcaa ctttgagttc aagaaaacat
    22921 agtgaagata tgagagataa taattttttt gaccatgtaa ataagaagaa tgaaactcca
    22981 tttgacagaa tgaaaagaga aggtatagta tatacttcag ctggagaaaa catagcagca
    23041 ggacagatta atgcaatata tgcgcatgaa gcttggatga actctgaagg acatagaaaa
    23101 aatattttgg ggaactataa taatattgga gttggagtaa tatttggagg aagctataaa
    23161 acatattaca ctcaaaattt ttataaataa ctaaaatatt tgaaaggaag ttaaaaaatg
    23221 acatcatgga aaaaaaagac agtatataag tgcttaatag cagtagtctt gttttgtgga
    23281 atagtgttaa tatctaattt tagtaaagtt tctgctttga tgatggatac taatggaaat
    23341 gttttgatta aacatggttc aagggaaaaa aagctaatag caattacctt tgatgatggt
    23401 cctcatccta aagaaactag tcaagtttta gatgtattaa agaaatacaa tgtaaaagct
    23461 acttttttta tagctggaaa acatgcaaaa tggtataaag aaccattagt aagagcaagt
    23521 aaagaaggtc acgagatagg aaatcatact ttcaatcatc ctgatataag taatttaagt
    23581 tctagtcaga tagaagagga gattgttaag tgtgaagaca ttttgaaaga agtaactgga
    23641 aaaaaaccga cattatttag accaccattt ggaagttata gagaaaaaga attaatagaa
    23701 attgcaaaaa agcatgacta taaagttgtt ttatggacag gtgtagatgt gaaagattgg
    23761 aaaaatccag gagctaatag cattgcagat aaaataatta ataaagtaca aaatggagat
    23821 ataatacttt tgcatgatta tgcaactaat gatacagttg aagctttaga tatgtttatt
    23881 ccaaaaatga ttgaaaaagg ttttaaattt gtaacagttt ctgagttaat aaaataattt
    23941 ttagggggga gatttacagt atgattctta tgatagacaa ttatgattct tttgtataca
    24001 acctagtcca atatatagaa gaattagggg aaacagttgt agtaaaaaga aataatgaaa
    24061 tcaaaataag tgacattgaa gaactaaatc cagaggtaat tgtgttatct ccaggaccgt
    24121 gttctccaaa agaagctggt atttgcatag atattgttga gcattttaag gggaaaaaac
    24181 ctatactagg aatttgttta ggtcaccaaa ctattggaca tgtttttgga ggagatatta
    24241 taaaagcaca acagcctgtg catggaaaag tatatagtat caaccatact aataaaggag
    24301 tttttagggg acttaaaaat cctttaaatg tcactaggta tcattcacta atagttgact
    24361 ccaatacagt tccaaaagaa ttagaaatta cagcaattac tgataaaggg gaaataatgg
    24421 ggattaggca taagaaatat ttaatagaag gagtacagtt tcatccagaa gctattttgt
    24481 cagagtatgg tcatgaaatg cttaaaaatt ttataacaga agctagagaa agggtacata
    24541 tgtaatatga taagagagat aaatactaaa ttaaattctt ttgagatatt tactattttt
    24601 agaaatgaac atgacagttt tatattagac agtgctatgg acaaagaaaa attgggaaga
    24661 tattctttta taagtagcca accatttaag gtattaaagt ataaagatac tgatgaaaat
    24721 cctttagaag ttttaaaaga agaactacat aagtataggg ttgtaaatga tactaatctt
    24781 ccatttgtag gaggtgctgt gggatattta tcttatgatt taggtaatta catagaaaat
    24841 ttacctagaa ctgctgtaga tgatattgag atgcctgata tgtattttgg attttataat
    24901 catgtaatag ttatagacca tcttgtgcaa aaaacttata tagctactcc aaacatagat
    24961 atcgaattag aagaaaagat aatagatgat atagaacaaa gaatattaaa agaagagaaa
    25021 aaaggtatag atagcatatg ttatgaagaa aaagaagtaa cacctataag acttaaatct
    25081 aattttacaa aggaagaatt taaaaatgca gttcaaagtg ttagagagta cataagacaa
    25141 ggagatatat atcaagctaa tttaacacaa aggttcagtg gagaaacaga acttacaagc
    25201 tttgaattgt atagggactt aagaagattt agcccagcac catttggggc attcttaaac
    25261 tttgaagatg cacatatatt atcaaattct ccagaaagat ttataagatg tgttaataag
    25321 agaatagaaa ctagaccaat aaaaggtact cgtccaagag ggaaagataa agaagaagat
    25381 ttaagacttc aacaagaact tagaaatagt gaaaaagata gagctgaact acttatgata
    25441 gtagatttag aaagaaatga tattgggaga atatctaaaa ctggaagtgt taaagttcca
    25501 gagctatttg taattgaacc atatgcaaat gtaaatcact tagtttccac agttgtaggt
    25561 gagttaaaag atgataaaga tgctactgat gtaataaaag caacttttcc aggggggtct
    25621 ataacaggag cacctaagat tagagcaatg gaaattatag acgaattaga gccaacgcaa
    25681 agaaatgtat atactggttc aataggttat ataggtttta atggtgatat ggattttaat
    25741 atagcaatta gaacaataat caaaaatgac aaaaaagtct attttcaagt tggaggagga
    25801 atgacttggg attctgaccc agatgaagaa tatcaagaaa cacttgataa agctaaatcc
    25861 ataatgaagg ctttgagggg atattatgaa gaataatgta ggttttgata gtagtttaag
    25921 taaatttgga attggtttgt ttgaaactat tagagttaaa aatggaatag cagtagattt
    25981 agatatccat atagaaagaa tgctaagttc aataaattgt ttggatttag atataaaata
    26041 taaaaaagat ttcttgataa atgaaattgt tacatatata aaaaaagaaa atgtcattaa
    26101 taaagcactt agaattactg tttttgatga aggttataat atatctatta gggatattcc
    26161 atataatcaa gagacgtatg agaaggggtt taaattagct atatctccaa tagttagggg
    26221 gaatagttta attcatagac ataagactac taattatttt gaaaacatat ataccaaaaa
    26281 tatcgcaaat aagactggtt ataatgatgg tatattttta aattcagaag gtgtaatatt
    26341 agaatgttct atgagcaata ttttctttgt aaaaggagaa agagtgtaca ctcctagtga
    26401 tagattaccg atattaaatg gaataataaa aaaaaggatt atagagattt gtgacgaatt
    26461 acatatagag ttgatagaaa atgaaattaa tatatcagaa attagtagtt ttgattttgt
    26521 ttttgtcaca aatagtctta tgggcgctat aaaggttaca gaaattgaca aaataaaatt
    26581 taataaagaa aatgttgttt ttgataaaat agtgaagtta ttagaataaa ttaaaatatt
    26641 aacaatttgt tggagacaaa agatgatagg ttgtatttat gaagtataca atgaagagat
    26701 aactaaaata atagaaaacc aaaacacttt ggctgtatat ttagtgggtt ctagtaaaga
    26761 cgtagatttt actctttatg atgtagagat aaacgatatt gatgtgttcg tttttgtaaa
    26821 agaaggggaa aaacaagaga gaatactgtt taaaaaaaag ggaatagaat ttgatgtaaa
    26881 ttacttttca aaagatggtg taaaaaaatt attatcaaat agggaatatt tctttgtgaa
    26941 agaaatgaaa gatgctaaag tactgtttga tagggagaat atatcacata ttataaaaga
    27001 tataagtaga aatatttact tagaaggacc cagtaaaatg tcattagagg aaaagtcttt
    27061 tataaagcaa gatattggag caaaaatatc gaatttaaaa aataaagaaa aatttgatgt
    27121 ttttgaatat cattttttga cgaatttata tttaaaagat ataataattg ggtactttaa
    27181 tataaatgat aaatgggtac caaaagacaa aaaattgcta aaagttttaa aaaaagaaaa
    27241 taatgagctt tttgaactag tgtcaaaagt aagtgaaaat tatgactatc aaagactttt
    27301 agatgtttat aattatattt ttaaagaaat tgagacaaaa gaggttataa aactaatctt
    27361 ctagtcacaa atcaatgggg ggagtttaaa tgaataaagt tgataaagaa aagatacaac
    27421 atgcagtaag agaaatacta gaggcaattg gggaagaccc agatagagaa ggtctaatcg
    27481 aaactccaaa tagagttgct aggatgtatg aagagatatt ctctggtctt agtgaagaac
    27541 caagagacca tttaaaagtt ctttttgcag atgaaaaaca tgaagagtta gtattggtaa
    27601 aagatattcc attttattca tgttgtgagc atcatctagt tcctttcttt ggaaaagctc
    27661 atattgctta tttaccaaaa ggaggaagac ttacagggct ttctaaactt gctagagtaa
    27721 tagatacatt ggcaaaaaga ccacaattac aagaaagaat cactaaaaat gctgcagata
    27781 taataatgga agaattacaa ccttatggtg tcttagtagt agtagaagct gaacatatgt
    27841 gcatgacaat gagaggcgta aaaaaaccag gttcaaaaac tgttacatca gcagtgagag
    27901 gaatatttga aaaggacatt gcgtctagag ccgaggctat gagtttgatt actatgaagt
    27961 agcaatttat tatagatata atcatttatt gagataaaat ttaatttaag ggagtgtcat
    28021 taattgtgtt tgattatggt aaaagaactt atataatggg aatattaaat gtaactccag
    28081 atagtttttc tgatggagga gattttaata acttggacat agcaatacaa catgcaaaag
    28141 atatggttga ccaaggtgca gatataatag acctaggagg agagtctaca cgcccaggtc
    28201 atagctatgt agactcagac gaagagctaa gaagagtaat ccctgtaata aaaaaactta
    28261 aacaggaatt ggatatacca atatcaatag acacttataa ggcagatgtt gcagaagaag
    28321 cattaaaatt aggtgtcact atggtaaatg atgtttgggg tcttagaaaa gataaaaata
    28381 tggcttctgt aataggaaaa tatgatgcag aagtgtgtat aatgcataat caagatggga
    28441 caaactatga caaagatata atggagtcaa taaaagactt ttttaaggta agtatagaaa
    28501 tggcaatgag ttgtggtgta aaaaaagaaa aaatagtact tgacccaggt gtaggatttg
    28561 gcaaggattt tgaacaaaat attgaagtat tgagaagatt aaatgaatta aaagatttag
    28621 ggtatcctat tttgcttggg acatccagaa aatcagtaat aggaaaagta cttccagttg
    28681 agcctaaaaa aagattagaa ggtacaatag ctactactgt tcttggtata agagatggcg
    28741 tagatatagt tagagtacat gatgtttatg aaaatttaat ggcagctaga atgactgatg
    28801 caatatatag aaagtaggta taatataatg gataaaatat tacttagtaa tctaggattc
    28861 tatggatatc atggtgttct taaagaagaa aattttctag gtcagaaatt ctttgtcgac
    28921 atggaacttt atattgattc aagagaagca ggtcttagtg acgatataaa taaatctgta
    28981 agctatgccg aggtttacaa tgttgttaaa gatattactg aaaataaaca atttaatctt
    29041 ttggaggctt tagcggaaaa tattgctgaa gaagtcttga ataaatttat acttataaat
    29101 ggagttatgg tcagagttag aaagcctgag gctcctgtaa atggtattta tgactatttt
    29161 ggtgttgaga taaggagaac aagagatgaa taaggcttat ttgggaatag gtactaacat
    29221 gggtgataga tttgataatt tatcaagagc atgtgaactt ttgaaaaata gtgattctat
    29281 atataaagta aaagaatcaa gcttatacga aacaaaacca tggggatata cagaacaagc
    29341 agattttcta aatatgtgtg tagaaataga aacagagttt gaaccttatg agttgttaga
    29401 atattgtcaa gaaatagaga gagaactaca cagagagaga atagttcatt ggggaccaag
    29461 aactatagat gtagacgtac tattttttaa tgatgtagtt tccactgatg aaagactaac
    29521 aatacctcat ccaaggattc aagatagagc tttcgttttg ataccactta tggatttaaa
    29581 tgaagaactt ataataaatg aaaaaacaat aaaagaacat ttaaacctcc tatctgctga
    29641 agaaagagaa gaagtaaagg agcttgtagg ttatgaaaga aaacctattt aataataatt
    29701 caaagataga gataaataca aataacaaaa agataactgt aaaaaaagat aaacttataa
    29761 tagctggacc atgcgcaata gagagttatg agcaactttt ggagactgct aaatttgtaa
    29821 aaagtcaagg agcaaacatt ttaagaggtg gagcatacaa gccaagaaca tcgccaaatt
    29881 catttcaagg attaaaaaaa gaaggtcttg aaatattaaa agcagttaaa gacgaagttg
    29941 gtatggctgt tattacagaa cttatggatg taagagatat ggacgaactg tatagtataa
    30001 gtgatataat tcaaattggg tcaagaaata tgcaaaactt tactctttta agtgaagtgg
    30061 gaaaacaaaa taaacctgtt atgttaaaaa gaggaatagc atccacaatt actgaatgga
    30121 taggtgcttc tgaatatata gcaatagaag gaaatagtaa tattataatg tgtgagagag
    30181 gtataagaac gtataatgat tatactagaa atacattaga tttagctgcg gtacccatta
    30241 ttcaaaagga aacaggtctt cctgttgtgg tagacccaag ccatgccact ggagttagat
    30301 atttggtaaa acctatgtca ctggcatctt ttgcatgtgg agcagatgga ataatggtag
    30361 aagtacatcc tgacccagaa aatgctttgt cagatggtgc tcaatctctt tgctttaatg
    30421 aatttgaaga tttaatgaaa tcaattaata attattaaaa gaggcaagag cctctttttt
    30481 attcgtaatc gttgctaagg taaatttata agttataatt tgatagttag attatttaat
    30541 ataaataaag atttaatatt gatagaatta atataaaaaa taaaaatttt ttgattaata
    30601 agaaggatat caataaaaaa agtcgaattg tgtatattga gtgattgaca aatttagaat
    30661 atagtgtaga atagaaatgt taacacaaaa aattacacca gaaggtgata atatgaatga
    30721 tataaaagac attattgaag aaataaaagc tagatgcgat atagcaagta taatatctga
    30781 atatatgaat ataaaacaat ctggagctaa ttacaaagga ttatgtccat ttcatggcga
    30841 aaagacacct tctttttata taaatacatc aaaacagatt tataagtgtt ttggttgtgg
    30901 tgaaggtgga gacgtaataa attttgtcat gaaaatggaa aatttggatt ttatggatgc
    30961 tgtaaaaatt ttagcaaata aatgcggtat agaaattaac actaacatga atgaagaaac
    31021 taggattaaa atagaaaaat ctaagaaatt tcaagatatt catacggaag cagctagatt
    31081 ttatctttca aacttattgg gaagcaaaaa tcttgggtat gaatatttaa gaataagagg
    31141 actagatgat aaaattatta agaaatttgg tttaggattt tctttggatt catggaattc
    31201 acttatgaat actctaatca gtaaaggata taaaaaacaa gacttattag agtgcggtct
    31261 aattgcaaaa aatagagatg gaactaattg ctatgataaa tttagaaata gagttatgtt
    31321 tcctatattc gattatagag gaaatataat aggatttggg ggaagagtat tagacgattc
    31381 tttgccaaaa tacttaaatt ctccagacac attaattttt aataaaaaac agaacttata
    31441 cggattaaat tttgctagaa aaaatttaga aagtaaaact atagtcttag ttgaaggata
    31501 tatggattta atttcacttt atcagtatgg tataaaaaat gtagtagcaa cccttggaac
    31561 agccttgact gaacaacaag gcattttaat aaagagatat gcggacactg ctattatatc
    31621 ttatgattct gatgaagcag gaataaaagc gactttaaga gctatagata tacttactaa
    31681 attaggtatc aatgtaaaag tgttagactt aaaagatgct aaagacccag atgaatttgt
    31741 aagaaaatac ggacttagtg attataaaaa agctatggat gtttctactc actacataaa
    31801 atataaaata gatcatctta aaaaagaatt taacattcaa aaagatgaag aacgagtgaa
    31861 gttcgcaaaa gaagcttcta aaattataaa acaattgaca agtcctgttg aaattgattt
    31921 ttatacaaag tatttaagca atcaaataga tattaatgtc gagtctataa agagagaagt
    31981 ttatggtaaa aattacaata agccatataa taacaaaaat caaaagaaaa tagaagaaaa
    32041 agtaattgaa aaagtcgaag tcagacaaga cggaaaacaa ttagttgaag aaactctaat
    32101 aaaaataatg ttagaagaca aaaaaattag agaaatagcg ttacttaaag tagaagaaag
    32161 tgatttttta ttgaaagaaa gtaaagaaat tttaaattac atgattaaaa atcaagaatt
    32221 ggacaaaata actattgaca aattgaaaag tttaaacata tccgaagaat acttaaaaga
    32281 attaaattca atttctttga atagtataaa cttagagaac acaaaagaaa tagaaggaat
    32341 aataacaaat atcagaaaaa actcattaga agagcaaatt aatagcttgt tgagagaaca
    32401 acaagaatta gaaaataata atgatatgaa agaggtagat ggtagagtta tggaaattgc
    32461 tttaaaaata gttgagataa ataaaatttt aaagagcttg tagagaaagg aggtttgagt
    32521 gtggaaaata aatcgaataa aaaagagtta aaaaaggtta ctgctaagac attaatagaa
    32581 aaaggaaaaa agcaaggttc tctgacactt gcagagataa tggaagcttt ttcggagact
    32641 gaacttgata aggatcaagt agaaaatctt tatgagactt taggaaattt gggaatagaa
    32701 ataacagaaa caaaaaacta taaagctgat atagattttt cggttgctga tgacgattta
    32761 agtataggcc acttagatga agatgcagag gcaatttcac atgatgactc ttctgcaata
    32821 gaaatagaaa ctgtggattt atctttacca aaagggataa gtatagatga ccctgtaaga
    32881 atgtacttaa aagaaatagg aaaaattcct ctacttaaac cacatgaaga agtagaattt
    32941 gctagaagga tgcacgaagg tgatgagata gcaaaacaaa ggttagttga agcaaactta
    33001 agactagttg taagtatagc aaaaagatat gtaggaagag gtatgctttt cttggattta
    33061 atacaagagg gaaatttagg tcttataaaa gcagtcgaaa aatttgacta tacaaaagga
    33121 tataagttta gtacttatgc aacatggtgg ataagacaag ctataacacg tgctattgca
    33181 gaccaagcta gaactataag aataccagtt catatggtag agactataaa taagctaata
    33241 agagtatcaa gacaactact tcaagaactt ggaagagatc caaaaccaga agaaattgca
    33301 aaagaaatgg aaatgacaga agataaagta agagaaataa tgaaaatagc tcaagaccct
    33361 gtgtctttag aaacaccaat aggagaagaa gaagatagcc atttaggtga ctttattcca
    33421 gatgatgatg ctccagcccc agcagaggca gcagcatatt ctttattaaa agaacaaata
    33481 gaagatgtac ttggttcatt aaatgataga gaacaaaaag tattaaagct tagatttggt
    33541 cttgaggatg gtagagccag aactcttgag gaagttggaa aagagtttga tgtaactaga
    33601 gagagaataa gacagataga agcaaaagca ctaagaaaac taagacatcc aagtagatca
    33661 aaaaaactta gagattattt agactaaaaa gatacatcat tgatgttttt gtgataaatg
    33721 ccatgagata agtgatatct catggctatt ttgtagtaaa gatttaattt tatttttgta
    33781 gttatttcag tctaatatct ataacttgga ttaaatccta taaataaagg gaattgaaag
    33841 gagaattata tttgaaatta acagatagat tattgaaaat agcatcatta gtgtcagatg
    33901 gtaaaaaaat agctgatata ggaacggacc acggatacat accagtatat ttgttaaaag
    33961 agggaaaagt accatttgca gtgctagcgg atgttaataa aggacctctt gacaatgcac
    34021 ataaagaagt catacagaac aatcttttag ataaagttga tttacgattg ggttctggta
    34081 tagagatact tgaaataggg gaagtagaag aagtgataat tgctggtatg ggtggaatct
    34141 tgataagtga gcttctcgaa gctaaaaaag aagtagcaca taatgtagaa aaattaatat
    34201 tacagccaat gcaagcacaa gaagaactta gatattacct tttaaacaac ggatatgaaa
    34261 ttttagaaga agttttagtg agagaagatt ttagaatata tgagataatt gttgccaaat
    34321 atacaggtaa aaatactata atagaagatg agatatatta tgaggttgga ataaagcttt
    34381 tagaaaacaa agattctctt tttaatgatt ttatagaaaa aaagataaag acatatagtt
    34441 ctatagttaa taaattagaa gggaaaaatg gggaagctat agataagaag agaaaggaaa
    34501 gcgaagttgc aataaaaaaa cttgaaaatt taataaaata aataggaggt atatatgcta
    34561 ttaaagagtc taactagaaa aattgaaaaa aagtatcctc taaacttagc agaggattgg
    34621 gataatgtag gtcttatagt tggagatttt gatatggatg taaaaaaggt attggtatct
    34681 ttagaagcta atgaagatgt tattgatgaa gctatatcca aaaatataga tttaattgtg
    34741 acacatcatc cttttatatt tgggaaaatt aataaaatta atagtggaga tttaaaaggc
    34801 agacttatcc aaaaacttat taaaaatgat atatcacttt attctatgca tacgaatttt
    34861 gatatagcat ttgatggtct aaatgactat tttatggaaa ttatggaatt tggtaattca
    34921 aaggttttag atataactaa aagcgaaaat ttgtataaat tagcagtata cgttccacat
    34981 aactattcag atgaacttag aaaggtttta agtaattcag gagcaggtca tatagggaat
    35041 tatagtgatt gtacgttttc aacagaaggt gaaggacaat ttaagccatt agaaggctct
    35101 aatccgtttc ttggaagtgt aaatgatata gaaactgtaa atgaagttaa aattgaaact
    35161 gtagtaccac agaagctttt aggtggggta ataagttcta tgttagatgc acatccttat
    35221 gaagaagttg catatgattt gtacaagtta gaaaataaag gtgagatatt tggactaggt
    35281 agaataagta aacttgataa aagtatgaca ttagaaagtc tttcaaggaa aataaaagag
    35341 aaattaaata tgaaacacat aagagtagtt ggaaacctta gtacagacat aacgaaagta
    35401 gccgttgtaa caggagctgg ttcagaattt gtaaaaaaag ctaagagaca aggtgcagaa
    35461 gtactaatta cgggtgatgt aaagtatcat gaggcacaag atgcacttga tattggaatg
    35521 tgtatagtgg attgtggtca ttttgataca gaagatatat tcaaaaatgt tatgaagaga
    35581 tttttagatg aatttagtga aattgaagta ataaagagta atgtatattt gaatccattt
    35641 agtactattt agcagtgttt gataataaaa atactatata aaaaataaat ttgagtttaa
    35701 ttagggggat aatgatgaaa gtagcagtaa tttttcatag tgtttgtggg agtacatatt
    35761 tattagctag agaatataaa gaagcgcttg aagaaatgaa tatcgaggta gatatattta
    35821 gagtaagtga cgaagttgca aaaacacttc ctcaatatta tttgataaat tccaaagaat
    35881 acaaagacga atttgaaagt ataaatgtaa ttaaatctgg aaaagaaata ttagattatg
    35941 atgctatatt tatgggttca ccaacatatt atggaaatgt atctggacca atgaagatgt
    36001 ttatggatag tttttctgat atttgggtag gtgctccttt gagtggtaaa atatttggtt
    36061 gttttgctac agcaggttca caacatggag gaggagaatt agctttgcaa gctatgaata
    36121 tatttgccca acatatggga atgactcttt tgtcagttcc atgttcagta agaggaggtt
    36181 atccagcgta tggaatactt catatagcag gagataattc tgatataagg ccaaatgatg
    36241 atgccaagat aggtataaga gattacctaa aaagacttaa catataagaa tatcatataa
    36301 aaatacctaa aagcgactgt atatactttt aggtattttt acttaattat aaacgtgaag
    36361 ctttaccaga aatatggtca atttctattt taacaacagt tgttaagttc atatccttct
    36421 ctatgtactt catgccttct tcaaagaatc ctttagaata tttttttatt atttctacaa
    36481 gagcattttt cttttcttca ttttctacag tgcaagcttt tccaaaaact atagcactag
    36541 aataagtagt actaaattta tctggaataa cactttcatt agcaactact aaaaaagaaa
    36601 ctttgttgtt tttcgatata ttatctaact tatgcccatt tcttgcacaa tgaaagtaaa
    36661 tagaatcatt aaagtataca taatttacag caactccata aggataacca ttttcagata
    36721 tagtggaaaa agtaccaaat tcaccatttt ttaatacttc aacagtatct tcttttgtca
    36781 tttctctttt tttcaatctc atttctttaa acataatatc atcctttcta attttgatgc
    36841 ttaaattaaa ttttagaaca tttataaaga ttatgctatg ttttttacaa attttaagaa
    36901 aaaaatatag tttaagataa aaggtagtga aagagaagct atttggaggt ttatttatga
    36961 tattattgtt agagtagtac aaataatact aataaattta atagctggtt tcttgttatt
    37021 taaggtagta agcatgtcta taagaagggg aagttatcga gggtagaagt tgttgtttat
    37081 aacgatatta taaagaaaat attattatat aaaataataa atagtaaaga ggtatcaagt
    37141 tgaatataat acttaatacc tcttcaaaat taagttataa aagtgttgtg ggaattgtcc
    37201 tatacatggt aaggagataa taaagaatgt atctttaaag tctcaactaa acagcaaaat
    37261 ttatccaaca aacatacaat aaatgcttct ttgtatctag gtggcttaac agtaagtggc
    37321 cacatgtgtt ttaaaattat gtccgtttct ttttcattta tttgaaaaaa taaagttgca
    37381 ttttttaatg cttctctagg gtgagtaaat ccatgtaatc cttttctatc acctttgtaa
    37441 tgccaatcat aaagaaaaaa gtcgtgtagt aaagctcctc taataacact ttgaatatca
    37501 acacttaagt gcaatttttt acataataga taactataat atgctactga caaactatgt
    37561 tcaagacatg aaacattgcc atgatgagga aatgttctca taagttgaac tttttcagaa
    37621 gaaagtatct cagaagcaca ttcattaaaa tatttgattt cagcttttga caacattatg
    37681 aaaatctcca ttcttagtaa tatataaata ctatacttca acgataatat gaaatatttc
    37741 ttatcctaca aaatataata cccaataata caagtaagat accttttatg gtattttaaa
    37801 gtaagagaga ttttattagg ctaaacaatg aaaaaattaa aagtaaagct atagcaatat
    37861 atagtatttt tccaagagga ttgccaaaat ttatagtcca tccaatacca aaccttttat
    37921 taaccattaa tgatgggtca tttggattat tatacatgat gcctgctatc caatacttct
    37981 catcttcttc tggagaataa gatgagttga attttaaatt tggggaatta atatatgttg
    38041 ctattaaata aattataact aaaagcgttg taatattcat agcagttaat agttttgttt
    38101 ttattgataa aagagtagta aaaaactgtg ttgtcattat aatcatcaat attaaaaaca
    38161 aataaccaat tttatttaaa tatttaatgt tttcaactct actttcttca attctatttg
    38221 tatctatctt aatccttgac tttatggatc caatggaagt tatatataat aaaaccatta
    38281 aacaaaagtc agtaccgatt aatttaaata cattaagaaa tgatttatct ataaaaacat
    38341 caggagaccc atttatgttc caatgagtgg caattgagtc tggcaattga ttataattaa
    38401 atgctataaa aatactcatt cccaaagtaa atagtatcgg tattaaatag attattttaa
    38461 atttttttat tattttattt ttcttattta taaaatccat atctactatt aattttgaat
    38521 ctatatctat atgacctaat ttagcataaa tttctgattt aagcttctta gcttttttat
    38581 gtgtatgaat atatagtata ctttcatata ataaaaaacc tataatacca ataaaatagg
    38641 aaagttccaa tttattaaat gtaaagacca gaataaaaac taatataaag tctataataa
    38701 aacctaaaga taatagtttt ttaaattctt tatctaactt tataaagtaa ttataatttt
    38761 tatgctcttg gtctatagaa actccataga tgtgtttttt acctacaaaa tagggtataa
    38821 aataaaatgt agcatacatc aaaattagaa ctggaaatat tgtaaataga cattcaatct
    38881 tactcataat atttcacctc atacttgtca aataaacttg aacttagtga caaaaattct
    38941 tctttactaa tacctgataa ataagatttt gccaccaata attccaagtc aagtttattt
    39001 ttttgacgag ttgtattatc tattttcatt ggtagttgag ctacaatagc accttttctt
    39061 ctatcaatat ttaagaatcc ctcttctttt aattcattgt aggatttgtt cactgtatgt
    39121 aaatttacac caatattttc agccatactt cttacagatg gaagtgattc atttattttc
    39181 aattctcctg aagctatagc cttagttatt tcttctttaa tttgtacata aatagattta
    39241 tcactattaa aatctaaatt taaaaccata aaaaacctcc attatgcttt ataaatgaaa
    39301 acgattgtta tatacatagt ataacataaa aaataataac aaacacaaaa tataagttta
    39361 tattttgtaa aaattgtaat ttaaaaaata acataatttt tattaaaata atacagatac
    39421 tatttataga tagaaataaa aattagaaag gagaaaaaat atgttaacat atttaatgaa
    39481 aagacaaatg cataaaaaag atgatggaat gagtaaacca atgatgatta caggagctgt
    39541 aattacaggt gtaggagcat atgctgtata taaaatggta aaaagccata aatctaattc
    39601 tcaaatggtc aatacaaatt attatggaaa tagcgattat gatgattata aatatgacga
    39661 atttgattat gactgtgatt gtagagacac aaaacaagat tatgaatatg aagaattaaa
    39721 aaaaaaagta aaagaattta attcaagaag aataaattgt gaaaattgtc cacatgtttc
    39781 tcaagaagaa atgatgcaat atgtaaatag tgttaaagat gtaaaggaag agcttgattt
    39841 acatgaagat gctaaaaaag ataatattta caaagaagat gaatacaaaa aatatgaaga
    39901 agaataagta atattttgta taactgaaca aatagtaatg tcaaattatt taatatctag
    39961 ttttaatatg taagttgtaa cttaacttga aagaatcagt atcattcttt agatagttaa
    40021 gtaacaactt acattttatt tttataaatt ttaatagttc tttgtgacta ttttgtaatt
    40081 ttagaaatat tttagtatcc agggtaattg tgtataataa aacttgtata gatagatagt
    40141 atgaaattaa gcagatagtt taaaattaaa atgaaaagaa aggaaatagt tatgttaaag
    40201 ttgttgatag ttgaagatga tagtactata tcttttggaa taaagtatgc tttagaacaa
    40261 gaaggattta gtatagatat atcaaaggat ttatctagtg gaaaagaaat gatttcttct
    40321 aatgaatata gcatgatact gttggatgtt acattgccag atggaactgg ttatgagctt
    40381 tgtcaatata taagaggatt ctctcaggtt cctataatat tcttgactgc ttgtgatgaa
    40441 gaagtaaata ttgtaatggg tctcgatata ggtggagatg attatataac aaaacctttt
    40501 agaattagag aattaatatc cagaataaaa gctgttttaa gaagaaaagg taatacttca
    40561 gaagaaaata agaaaatact taaatttgga gatttaagta tatatacatt agaagctaga
    40621 gtatataaaa atgataaaga aatatttctg acatcagttg aatataaact tcttttaatt
    40681 cttatacaga ataaaaatac tgttttaagt agaactcaaa tattggaaaa actttgggat
    40741 gtaacttatg attttgtaaa cgacaatact ctaactgttt atataaaaag gcttagagaa
    40801 aaaataggtg atgatttatg tgaaccaatc tacatagaaa ctgtaagggg gattggttat
    40861 aaatggatag gaagtgaaaa tagtgtttct atataatcca gaagtgaaga agtttttaag
    40921 taagtatgtt actttgatgt ttattgttat cataatatct attggattta gcataatcaa
    40981 cgtgtcctta actaaagaga tggttgtaag aaataatcag gctataatag gaactttatc
    41041 aagcaagtat ccaaacttag aaagtcagat agtagacata ataactcaag ggaagtctat
    41101 ggagaacata gattatggtc agaaaatatt aattaagtat aattatgata aatctattag
    41161 gataaactct gagcctatta tttctaaatt agttttagag actatcaaaa taaatataat
    41221 attagtatgt ataatattta ttttgatatt tgtgttgatt gttagatatt ttacgtcaat
    41281 ttacaatgat ttatcagata tgactaagta tgtttactat agttcagagg gtaagccatt
    41341 tgatatgaaa aacaagaatc aagaagggca gataggtctt ctaaaaactg aactcttaaa
    41401 aatgacgaca attcttaatg aaaaggttga acttttaaaa agagaaaaga tatttttaaa
    41461 caatacaatt tccgatatat cacatcaatt aaaaactcct atgacttctt taataatgtt
    41521 gaatgattta ttatataatg atgtaccata cgaagtaaaa atcgattttt taaataagat
    41581 aaaaaatcaa ttaaatagaa tggattggtt gattaaaagc atgcttaaat tatcaaaggt
    41641 agaagcaaaa gtgataaatt ttaaaaaaga taaggtaaaa tttagtgaac tcatacatag
    41701 agcaatgcta tctatgaaaa tacctatgga gattaaaaat caaaaactga ctatagaggg
    41761 aaatgataat gtatcttata ttggtgatat tgattggtcc gtggaagcat tagtgaatat
    41821 aatcaaaaat tgtgtagaac atactcctga atttggaaat ataattataa cctataaaga
    41881 aaatccatta ttttctgaac taataataaa agatgagggt gaaggtatag ataaaaaaga
    41941 tataccacat gtatttaaac gattctatag aggaagaagt agttcaaaag aagatagtgt
    42001 aggaataggt cttgcaatgt caaaatctat aatagaaagc caaaatggcg atatatatgt
    42061 aaacagcgaa aaaggtaagg gtacagaatt tcatataata tttcataaaa tgtatgatag
    42121 tgactaatct gtaatattta attcacttta tagtaatagc caaaacctaa tatatgaagt
    42181 atagaatata aatattggag gtagttatgg aaattttaag agtagaaaat ctaactaaaa
    42241 gttatggtaa aaatgaaaca aaagttgatg ctataaaaaa tgtaagttta tcagttgaaa
    42301 aaggaacgtt tatagcaata acaggtccaa gtggaagtgg taaaagtacc ttattacatt
    42361 tattaggagg agttgacaga ccaactagtg gtaaggttta tataaatgat gttgatatat
    42421 ataatttaaa aactaaggat ttggcgatat ttagaagaag aaatataggt cttatatatc
    42481 aattttacaa cttaattcca gtcttaaatg tgaaggaaaa catattgctt ccagcagagc
    42541 ttgacaatag agatattgat agagaatatt ttgatgattt aatgaaaaca ttaggtctaa
    42601 ttgatagaga aaagcacctt ccaaaccaag tatcaggagg tcaacaacag agaacctcta
    42661 taggacgtgc attaattaat agaccttcta taatattagc agatgagcca acaggtaatt
    42721 tagatagtaa aaattcaaag gaaatattag aattgttaaa attgtcagta aaaaaataca
    42781 accagacact aataatgata actcatgaca aaaatatggc acttcaagct gatagaatta
    42841 taagcataga agatggaatc ataaaaagtg atgaggtgat gtagatgaat ttatatacat
    42901 cactaactat tagatactta aaacaaaata aaagaagaac cattgtaacc ataatcggta
    42961 taatactttc aacagcttta atttgtggta taggaaatat atttgaaagt tttatggatt
    43021 atcaaatgag agaaactata aagaatgatg gaagtttcca tgttacattt tatgatgtaa
    43081 atagaaaaaa tgtggaatat gtaacgaaat cagctgaaat agaaaaacat gcatttacca
    43141 aacaacttgg atattctaaa ttagaaaata gtgaaaatgc tatattaagt ataaagcaat
    43201 atgataaaaa tgcaataaat ggatataaag tttctataaa agaaggtaga tttccagcta
    43261 aggaaggaga aattgtttta agtgaaagta ttattaatct tatagatgat aagttaaaaa
    43321 taggagataa gataacttta aaagtagggg atatgtttga ctcaagcaag aaaaagattg
    43381 atagtatgac atttcatgaa ggtgattata tagcaaatga aaaagataga acttttaaag
    43441 ttgttggtat aattgaaaaa cctgggtttg aacgttataa tggaattgca acagctatag
    43501 cctactttaa tcctatgaat aattacaatg atacaattaa tgtatcagta gcagtaaaaa
    43561 atcctaaaga tgtgtataag ataagtaata aaataagtaa aaatatatat tcaaataaag
    43621 ataaagaagc agtttctgat atgataaaat acaatgaaca tttattgagg ttacaaggtg
    43681 ctagtaagta ttcaaatata aatagctcta taaagtctat aattatagca gtaactattt
    43741 tagtaatcgt atgtacaatt gctacagtgt acaattcctt tagtatttct ataaatgaac
    43801 gaaaaaaaca gtttgggata ttaaattcta taggtgctac atctagtcaa attaaaagat
    43861 tagtttttat agaaggtatt ataattagtt taattggtat accaatagga ctaatatctg
    43921 gaacagtagc tatagactta ttgtttaaaa ttataaataa gtattttact gaatcggttg
    43981 ttactaagat gagtttgcag atagtgtata atccaataat aattattgtt agtataataa
    44041 tagttttgct tacaatcttt atatcaattc tactacctgc aatatcggct tctaatattt
    44101 ctccccttaa tgtgattaaa aatactggag agtataaagt gggcaaaatc aaaagctcaa
    44161 gacttattaa aatgattttt aaaactgaag gagtacttgc atataaaaat attaggagaa
    44221 atagaaaaaa atttataata actctttttt ctttaatggt aagtataata atatttatat
    44281 cttttagtgg ctttacatta cttttactaa gaggtgaaga aattagaaat tctcaaagaa
    44341 aatatgattt gtatttaact gcaaagaaca cagctaggtc tgtagaggat actataagtg
    44401 aactagaaga tattgatggg ataaaaaact ttgaattggc aacaggacaa tatgtgtcta
    44461 taagagtaag tgaaaataaa ataaataaat ccaaggaaga tttaatcagg aaatattatc
    44521 aaaaatataa gattggagat agttatgaat atgatttcag taataatgaa ttaatttttc
    44581 caggagattt tgcagtaaaa aacaaaataa ataacttagt acagggctct tttaataaag
    44641 agagagcaat tgaagaaaat ggtgttatat tagttagaaa aagtgcaatt gaagaaaatg
    44701 ggaagaaagg tgtagtagaa cttaccaact ataaagtagg agatacagta aattgtgaat
    44761 atcttgatga aaatgcctta agtaaaaaag taaaggtcaa gattcttgct ataactgacg
    44821 aagaacgtct aggtcttggt tatcaaaata tggggttaca atttataact tatgatgaag
    44881 ttgctaaaaa tttaaattta aaattaagta aaagccttat atttattgat tcaggaggaa
    44941 gcattaagac taagaaggaa atagaagctt tggcaaataa aaataatttt aacttttatg
    45001 atgaaagtgc aagcaatgaa agtgaaaaac aagatttaaa ggttattaaa atatttgtat
    45061 atggttttat agtagtgata tctttagtga gtgttacaaa tattctaaat acagtaagta
    45121 caagtataaa tctaagaaaa agagaacttg cagtaataca atctataggg gttactccta
    45181 aaggatttag gaaaatgata tatttagaaa gttttatata tggtatactg tcattgttgt
    45241 ttggaatacc aattagtatt ggtataattc taattatgaa taaacttatt tcaggtgtaa
    45301 ttgaattttc tccgattata ccgtggacag ccatagttat ctgtatagta agtgttttta
    45361 taataacttt tatagcaggg tatataccaa tgagtaaatt aaataaagaa aatataatag
    45421 ataatataag aagagaaag
//
LOCUS       DAMJYR010000020        44792 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.020, whole genome shotgun sequence.
ACCESSION   DAMJYR010000020 DAMJYR010000000
VERSION     DAMJYR010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 44792)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44792)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44792
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.020"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            116..292
                     /locus_tag="PC471_001054"
     CDS             116..292
                     /locus_tag="PC471_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2788193.1"
                     /translation="MEINVIEIFPKDKAKLNKIEMDKASWFVNIISKKYPKEALNEAF
                     STLEKELNISKANT"
     gene            466..1596
                     /locus_tag="PC471_001055"
     CDS             466..1596
                     /locus_tag="PC471_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003462216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HDF2788194.1"
                     /translation="MNNFEIKTRIKFGEGSLKALQELKNKNVLIITDPFMVKSKTIDK
                     ITSNLINSTYQVFEEIVPDPPIEVVVAGIEAFKSINPDVIIALGGGSAIDAAKSIMDF
                     SKKILKINEVEFIAIPTTSGTGSEVTSFAVITDSQKKCKYPLVSDDLLPDMAILDPEL
                     VKTVPNFITADTGMDVLTHAIEAYVSTNSNDFSDALAEKAIKLVFKYLIKAYKDGNDI
                     EAREKMHNASCLAGMAFNQASLGINHSIAHVLGGKFHIPHGRTNSILLPHVIEYNSSI
                     VGYTNTTYSATAKKYAQIAELVGLGSANIRISVRNLVNEIKKLQKEMNMPTKLTQCKL
                     SLEDITNEEREIAKLAIKDTCTLTNPKVPTEDDIINILKNIK"
     gene            1837..2187
                     /locus_tag="PC471_001056"
     CDS             1837..2187
                     /locus_tag="PC471_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003453843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HDF2788195.1"
                     /translation="MTEESKQRVIQEYVPGKQVTLAHIIANPNEDIYKKLGLVLDKKD
                     AIGILTITPSEASIIAADVATKASNVSLGFIDRFSGSVVISGDVSSVESALNDVLEVL
                     GNMLNFSSTKITRT"
     gene            2195..2644
                     /locus_tag="PC471_001057"
     CDS             2195..2644
                     /locus_tag="PC471_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutP/PduV family microcompartment system
                     protein"
                     /protein_id="HDF2788196.1"
                     /translation="MKNIIFMGKTGSGKTTLCQKLDQLELKYKKTQSVELYNNAIDTP
                     GEYMENRNLYNALITTSADAKIIAIVYDATQEENYIAPGFASMFCKDVIGIITKINKA
                     TENEVNIAYERLSLAGASQIFKIDTVDNIGLEKLFKYLEKYKNIDEV"
     gene            2746..3321
                     /locus_tag="PC471_001058"
     CDS             2746..3321
                     /locus_tag="PC471_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HDF2788197.1"
                     /translation="MGKKILLVDDEPLLRMDTKDILESHGYEIIGEASDGFEAVEMCK
                     KHKPDLVIMDIEMPILDGIKAGKIICKENIAGGVLLLTSFEDKEYVEKAKSIGAYGYL
                     VKTASEKSLIPTIEMCLNKVNEFEKMKKDLEKVNSKLNERKLVERAKGILIREFKISE
                     DEAYTRIRKLSMDKRTTMMEIAKMIIIGYDD"
     gene            3314..4723
                     /locus_tag="PC471_001059"
     CDS             3314..4723
                     /locus_tag="PC471_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase N-terminal domain-containing
                     protein"
                     /protein_id="HDF2788198.1"
                     /translation="MTNTIKKISTAQTYLNNNEIEKIIEVSKSLDLMASFYEADVFID
                     VLSKFDDEAIVVAHGRPKEKSMYCENVIGKKALIENEPGVIKCLKTGERVRDIKALTQ
                     EYKLVKQNIQPITLDDKVLAVLIVEKDISSEVKNEFNASEDEENKNLHQFIKLIGNND
                     FLVDNLNSAILIFDKNGKLKIKNRIATEIYKNLGFGYDIQDMHYNDLDIETNTFENIL
                     MDENYDETNEVSIGNMFFKIKTIYIKDDEFKVCKIVQDISDLKRKEEELVLKTVAIRE
                     AHHRVKNNLHTVISIIKKQSRLSQNDEVKQCLDNIINRVFAILSTHYLLSKEVDNNIS
                     IREGINLLVSNIQGGYLFSKDIDIRITGEDFKISGEKATAILLVINEILQNCYDHAFS
                     DREHGTIQILINKDNDYRNIAIIDDGIGFDSNNINKNSLGTYIIDSYITQMLKGNIER
                     KSSENGTEVLITIPTQINI"
     gene            4907..6340
                     /gene="eutA"
                     /locus_tag="PC471_001060"
     CDS             4907..6340
                     /gene="eutA"
                     /locus_tag="PC471_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004458698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase reactivating factor
                     EutA"
                     /protein_id="HDF2788199.1"
                     /translation="MREELLSVGIDIGTSTTQLIFSKLIVENTESNFSVPRISIVDKE
                     IIYKSKVYFTPLISNNEINGQQIKDIVENEYKKANISKEDIHTGAVIITGETARKENA
                     NDVLHTLSGFAGDFVVATAGPDLESIISGKGAGAHIYSKEHSTTIVNIDIGGGTSNIA
                     SFKRGEVTDTGCLDIGGRLVKIDKISKKITYISPKIDKIIKDNNLNLQVGTMATVENT
                     KPIVDIMVDLLMESIGLKKQTPLFDFIITNKSISIDENIKNVSFSGGVADYIYYDGEI
                     KDYFEYGDLGILLGQAIKNCEYLKKVNVVKSMETIRATVVGAGSHTTEVSGSTITYTS
                     EKFPLKNLPVLKLSIDEETGTSKQISEAIKNKLNWFKLENDLQKVAIAMIGKKNPTFL
                     DIQEYAKGFVDGMQQIIENDLPFIVIVENDMAKVLGQAIYALLNYEKEIVCIDSVKVE
                     NGDYIDIGKPIVEGKVLPVVIKTLVFN"
     gene            6358..7722
                     /locus_tag="PC471_001061"
     CDS             6358..7722
                     /locus_tag="PC471_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003454054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit EutB"
                     /protein_id="HDF2788200.1"
                     /translation="MILNTKLFGRTYQFKGLYDVMAKANEEKSGDKLAGLAAESAEER
                     IAAKVVLSHIKLEDIRNNPAVAYEEDEVTRIIQDAVNEKVYEEIKNWTVSELREWLLD
                     SNTTSLDIRRISRGLTSEMIAAVAKLMSNMDLVYAAKKIKVTAHCNTTIGEQGTLSAR
                     LQPNHPTDDPDGILASLLEGLTFGIGDAVLGLNPVDDSVESVTKVLKRFEEVKQKYQI
                     PTQTCVLAHVTTQMEAIRNGAPADLIFQSIAGSEKGNEAFGFNGDTIEEARQLALKHG
                     TATGPNVMYFETGQGSELSSEAHHGIDQVTMEARCYGFAKKYEPFLVNTVVGFIGPEY
                     LYDAREVVRAGLEDHFMGKLHGLPMGVDVCYTNHMKADQNDMENLAILLTTAGCTYFM
                     GIPHGDDVMLNYQTTGYHETASLREMFGLTAIKEFDAWMEKMGFSKDGKLTELAGDAS
                     VLLG"
     gene            7735..8616
                     /gene="eutC"
                     /locus_tag="PC471_001062"
     CDS             7735..8616
                     /gene="eutC"
                     /locus_tag="PC471_001062"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014952556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit EutC"
                     /protein_id="HDF2788201.1"
                     /translation="MNEKDLKALVEQLVGQMVGELDTNVVSETVKKATEVVVDNNACI
                     DDITEVDIRKQLLVKNPKDAEAYLDMKAKTPARLGIGRAGTRYKTETVLRFRADHAAA
                     QDAVFSYVDEEFIKENNMFAVETLCKDKDEYLTRPDLGRKFSPETINNIKSKFGTNQK
                     VLILVGDGLSSAAIEANLKDCVPAIKQGLKMYGIDSSEILFVKHCRVGAMDHLGEELG
                     CEVICMLVGERPGLVTAESMSAYIAYKPYIGMAEAKRTVISNIHKGGTTAVEAGAHIA
                     ELIKTMLDKKASGIDLK"
     gene            8636..9289
                     /gene="eutL"
                     /locus_tag="PC471_001063"
     CDS             8636..9289
                     /gene="eutL"
                     /locus_tag="PC471_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003761789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutL"
                     /protein_id="HDF2788202.1"
                     /translation="MKNDVIRPNILGIKIISNISPEMAQKLELKSHHKSLGLITADCD
                     DVTYTALDEATKASEVDVVYAKSMYAGAANASTKLAGEVLGIIAGPSPAEVRSGLNAV
                     VDFLEYGATFISANDDDSIAYYAHCVSRTGTYLSEVAGIREGEALAYLVAPPLEAMYA
                     LDAAMKAADVKMCELFAPPTETNFGGALLTGSQSACKAACDAFAEAVKSVADNPTGF"
     gene            9300..10001
                     /locus_tag="PC471_001064"
     CDS             9300..10001
                     /locus_tag="PC471_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HDF2788203.1"
                     /translation="MDAIGLIEVIGYVAAIEASDACLKSANVNIVRVDKVGAGIVTLT
                     ICGDVGAVKAALEAGEIAASRVGTLRTSHIIPRIHNEVTNALFKTKEPKVCEVSEDKE
                     KNFEITLEIADNVSKNLSEKENVILDVNENSGDTENLYSDIDKTNKKIETVDDSLTEA
                     NEEKIENTNDKEVLEQVKIENSDFDRDDLSKNSVKKLKAMAKKLDSSITYKDLNVLKK
                     EELINLVKKLSRRDR"
     gene            10004..11473
                     /locus_tag="PC471_001065"
     CDS             10004..11473
                     /locus_tag="PC471_001065"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetaldehyde dehydrogenase (acetylating)"
                     /protein_id="HDF2788204.1"
                     /translation="MTLLDKDLVSIQEVRTLVRRSKVAQEKLSKMSQEQIDKIVKAVV
                     DAAFNNATKLAKMANEETGFGKWEDKVIKNTFASMGVYETLKNTKTIGIIGEDKEANT
                     IDIATPVGVVAGLIPSTNPTSTVIYKTIISLKAGNTIVFSPHPNAKNCILETVNIISD
                     AAIKAGAPEDSILCITNPSIVGTNELMKHKDTNLILATGGEAMVRAAYSSGTPAIGVG
                     PGNGPAFIDKSANVKLAIKRIIDSKTFDNGVICASEQSIVIEKSMEETVLKELKNQGA
                     YVLNEEESERLAKFILRANGTMNPQIVGKSVEVIANLADLTNVPKNTKVLVARENKVG
                     HNVAYSREKLTPILAFYVEENVEAIMNRCREILLNEGAGHTFSMHVNDEELVKRFALN
                     MPVSRILVNTPSALGGIGASTNLVPALTLGCGAIGGSSTSNNIGPLDLINVKRVAYGI
                     KEIEELGRDKSQVEKSMFDSMDKEELINLLVQKLVKELA"
     gene            11569..11856
                     /gene="eutM"
                     /locus_tag="PC471_001066"
     CDS             11569..11856
                     /gene="eutM"
                     /locus_tag="PC471_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutM"
                     /protein_id="HDF2788205.1"
                     /translation="MASANALGMIETKGLVGAIEAADAMVKAANVQLVGKEQVGGGLV
                     TVMVRGDVGAVKAATDAGAAAAERVGELISVHVIPRPHFEVDAILPKVSAE"
     gene            11983..12744
                     /locus_tag="PC471_001067"
     CDS             11983..12744
                     /locus_tag="PC471_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin adenosyltransferase"
                     /protein_id="HDF2788206.1"
                     /translation="MGVLTENEVKRKIGKNKNNITEFYIEKGQIITPSAKSYLAERSI
                     ELKYVDKLSDVNNEPKEVVKEVVKEKKYRYTTVFGAKLDEKPEHMTQLNGNLLVFKDH
                     KRIILRGKIDSLESEILKVQILCQKLDMPKLVKELQEVLDFVRNIMRCEVLDEKLGDF
                     KLLGLTPQELREQSHYPKKYFGIGHEFPSYEMGEVVVAINSIRSNTREVELAAYEAFK
                     GEYGNVHREDLIKALNRLSSVFWIMIYKVRTNKYK"
     gene            12757..13386
                     /locus_tag="PC471_001068"
     CDS             12757..13386
                     /locus_tag="PC471_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate propanoyltransferase"
                     /protein_id="HDF2788207.1"
                     /translation="MENLLNEIVEEVITRVKKEAFIEVEASGRHVHLSREDVDKLFGE
                     GYTLTKLKDLSQPGQYACKERVTITGPKGSIKNVVVLGPCRNETQVEISLTDGSTLGL
                     KAPIKQSGDLEGTLSIKISTQYGEVELDKGLMVAKRHIHMTPKDAEKFNVCDKEIVEA
                     KVMGQRPLTFDDVVIRVSDSFKTYMHIDYDEANACGYSKGTVAKIIKKA"
     gene            13428..14153
                     /locus_tag="PC471_001069"
     CDS             13428..14153
                     /locus_tag="PC471_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02536 family ethanolamine utilization
                     protein"
                     /protein_id="HDF2788208.1"
                     /translation="MNYDNLVDLITEEIYKKINSNEIKISNKPKAVIVFEQDNNKFNL
                     LKDEFEIVEFDKSIRECEIVIVSRLCMRGLSNIALGNSTSDEERFILKMIMKGKEVYV
                     LDEGIEYKKYKDTAPKALYKKFMSFEDEICKFGVEIIKDLNVITKNKLNIKKELESTR
                     GSKKEDTKVLDKDVLNLTSVESVKIDNEFSLDLRNKKLISEADLRKPTINGVKNILVN
                     KKSIITPLAVDFMRIHHLKLKKV"
     gene            14166..14438
                     /locus_tag="PC471_001070"
     CDS             14166..14438
                     /locus_tag="PC471_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutN/CcmL family microcompartment protein"
                     /protein_id="HDF2788209.1"
                     /translation="MDIGKVVGNVWATRKDEKLCGQKLLVVKILKTKDTYKEGFMVAA
                     DNAGAGNGDLVLISKGSSARESIEKSHVPVDATIVGVVDSFEVFDE"
     gene            14431..14982
                     /locus_tag="PC471_001071"
     CDS             14431..14982
                     /locus_tag="PC471_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HDF2788210.1"
                     /translation="MNKSIGAIEFKSIAKGIEVSNEMIKKSSVDVLYLKSICPGKFLI
                     IVGGETSYINECVDYGIKLGEGYIVDNFVINAISQEILDGFKNKYQKLDSIVSIGVVE
                     SSKVCAGIKMLDKTLKSGDLVLVKLQLSFAIGGKLVYIVAGDLSSLEYALKESENVVR
                     EKEVIYKTVIPSVDSQIIKSLIK"
     gene            14994..16085
                     /gene="eutH"
                     /locus_tag="PC471_001072"
     CDS             14994..16085
                     /gene="eutH"
                     /locus_tag="PC471_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutH"
                     /protein_id="HDF2788211.1"
                     /translation="MSINEIIIYVMVVFMVLGAIDKCIGNKFGLGEQFEEGIMAMGSL
                     AVAMVGVICLAPVLADVLRPIVVPVFNILGADPAMFAGSLLANDMGGAPLALELAKDP
                     NAGLFGGLIVGAMMGPTIVFIIPVALGIIEKEDQKFLATGILAGIITIPIGAFVGGLV
                     AGFEVMMVLRNLVPIIIFAVVIALGLLKFENAMIKGFTYFGKGVVIVITLGLAAAIVE
                     ALTGIVVIPGMKPISEGIEIVGDIAIVLAGAFPLVFVITKVFNKPLMALGKVLGMNDV
                     SAAGLVASLANCIPMFGMMKDMDNRGKIINVAFSVSAAFVFGDHLGFTAGFNSNMITP
                     MIVAKLVGGITAVLLAMVIANKTLKKENV"
     gene            16087..16560
                     /locus_tag="PC471_001073"
     CDS             16087..16560
                     /locus_tag="PC471_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF861 domain-containing protein"
                     /protein_id="HDF2788212.1"
                     /translation="MDISNIDKNLIETLVRQIIEEKISGTKDTVDFVRNKDISGITSI
                     KLPTVKVSESDRLDTGNPSDVVYTKDLFTLEESPRLGCGMMEMKETTFDWTLNYDEID
                     YVIDGTLDIIIDGRKVSASSGELIFIPKGSKIQFSVPDYARFIYVTYPADWASQN"
     gene            complement(16680..16781)
                     /locus_tag="PC471_001074"
                     /pseudo
     CDS             complement(16680..16781)
                     /locus_tag="PC471_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433047.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
     gene            17076..17597
                     /locus_tag="PC471_001075"
     CDS             17076..17597
                     /locus_tag="PC471_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDF2788213.1"
                     /translation="MEEIVKKEQASMNDIFLYLKAKSNSLYKFVILYNEFINKPKDYG
                     TGELINMVEVHMLTAIEENPGISPTQLAKIWNRTKGAISQTISKLEKKGYITKEKKEG
                     NAKTILLYPTTSGVELSKAHKLYDTIDVTRTFEYLIKECTLEEIDSFYKVIDLFINLL
                     EESPQENNDSEKV"
     gene            17976..19151
                     /locus_tag="PC471_001076"
     CDS             17976..19151
                     /locus_tag="PC471_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HDF2788214.1"
                     /translation="MNLKQLSEKYEEYVIQMRREFHKYPELSFKEIRTTQRIREELQK
                     IGVPFVSIEPNIVIATIQGKNEGKTIAIRGDIDALPMQEDSDVEYKSEYDGIMHSCGH
                     DAHGAMLLGLAHMLNDIRKDLKGRVLLVFQAAEEVGGGHKEIIQYFKENGGPDRLIAT
                     HVWSDIEAGKISVEPGPRMAGGSGWRIDITGRGGHGSRPDQSIDPIKPACDIVLKVSS
                     IPVNHISVLEQCVVHACAIHGGTTIGNVIPNNAEVIGGYRYFTEESGKKVFDIIKQMS
                     NGVGQVYGVDVKVTHTGGIGPVINDEEAVKMAKEIVSNIDGLELDSYEPICASDCYGE
                     ILKEYSGFYCYLGVGNKEKGIIYAQHHPKYNIDEDTLKLGCEFMAKYAVEFLNKNNN"
     gene            19186..20724
                     /locus_tag="PC471_001077"
     CDS             19186..20724
                     /locus_tag="PC471_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HDF2788215.1"
                     /translation="MQGALKENKKKSGGGFLGWIEKVGNKMPHPLALFTYITLIVVAL
                     SAIASFLGFSATSPATGKLIKVMNLISAKGLVLFMQEFVKNFQNFPVLGVVLVMAVAT
                     GVCEKTGFFSTAIKMSLSKVKGNAVVFIIAFIGVFANQAGDAAFVLVPTLAGAIFYGL
                     NRNPLAGIFCGFASVGGGFATAVIPGGWDVTLTPITVSAAQTIQPAFDMTLLASYYAL
                     FVSAFIVATVSTIVTVKFIEPKLGKYTGKPEGIDDNQGFEVTKEQAKAAKLAGFTVLA
                     YVALLIALCIPANSFLRSPEGSLIFGAPLMSCLQFFIVILFFLPGIVYGMRVGKIKTV
                     KDLNDILCQSMAAMAPFIVLAIVIGQFLTLFSVSNLAQVLAIKGGALLNSLPLPPQAI
                     ILLFLVLVAFINIFVISGSTKWMIFGPVFVPMLMQLNIHPAFTQFVYRLGDSVTNHLS
                     PLNAFFIILLATVQKYDKNAGMGTIFSAMIPYTIGYFVVLAVLVVVWMTIGIPVGIGG
                     QVWL"
     gene            20756..21958
                     /locus_tag="PC471_001078"
     CDS             20756..21958
                     /locus_tag="PC471_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HDF2788216.1"
                     /translation="METTEIRYNFDEVIDRSGTNCLKYDLKSQFMPESPEDALPLWVA
                     DMDFACAPEIINAMHERIDRKIFGYSSQQTDDYYEAVCGWFKRRFDWKIEKENIFFSP
                     GVVPALGFFTQILTQKGDGIIIEKPVYYPFEDKITNNERKVVNSPLKFEDGHYSMDYD
                     GFEELAKDENNKVFILCSPHNPVGRVWKKEELRKIVDICKKYDLWIISDEIHCDIIRK
                     GEKHIPLEVACPDYKDRIITCTAPSKSFNLAGMQLSNIVINNKELQKKWMLETDSKLA
                     IFLPNPFAIVATKVAYNDCEDWMNQVNEYIDGNIKYVEDFVNEYMPKVKIIKSQGTYL
                     VWLDFRGYGLDAKQLEYVMLNKAKVLLDEGYIFGEEGAGFERINVASPRTIIEECMNR
                     IKCAFEGL"
     gene            complement(22125..22724)
                     /locus_tag="PC471_001079"
     CDS             complement(22125..22724)
                     /locus_tag="PC471_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDF2788217.1"
                     /translation="MNDNIVEVKNINMNFYTKEGELEVLKDVNFNLKEGEILTLLGPS
                     GSGKSTILNILTNLLKPTSGDIKITGNIGYMFQKDNLLEWRNIMDNITIGLEIQGKKD
                     KKSLDRVESLLKTYGLWDFRSMYPKELSGGMRQRVALIRTLSVNPDILLLDEPFSALD
                     YQTRLLVCDDVYSIIKNENKSTILVTHDIGEARCIKVQL"
     gene            22894..24252
                     /locus_tag="PC471_001080"
     CDS             22894..24252
                     /locus_tag="PC471_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2788218.1"
                     /translation="MSNIITNLIESIFTNPITILFLLAITVYSVFKIIENNRVYSFVS
                     SKLDEINRDYKTSEFYKKVRDDYYTYSKENPYADVNITSFIEEVVSDLKHNNLPLLEK
                     IRSIKNSSSISILLGVLGTFVGLSTMLLCVDTKDIINSLPSTISSMQTAFTTSIFGVV
                     FSLIIGYFTKIKDCEHVLIQIMLKSENLLTSEITHFKSERMDLKVEEVKNTIKQISKS
                     IEAIEKFDKISKDLNDFNDEFISGIEALKSLLEGSQSSIKTFDQSVRKLDKQFNILNI
                     KFVKLFDKYDNQDNINKEILFDIKESSKNIYNATESQFKIRDYIKNINAGFALYERSA
                     QDLLTKLMTHENKISQNQKILLDEKFTLDDSIKNLSSIIENFSNDLQVKLDMMFENSL
                     DIQDKLDVMFNNSFMNDEVPLDSEELFKDDVNNVFNPFSEEIYEIEDKEIKVIGEDEL
                     NE"
     gene            24245..24877
                     /locus_tag="PC471_001081"
     CDS             24245..24877
                     /locus_tag="PC471_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HDF2788219.1"
                     /translation="MNNKYRRTVNREFEKSSFWPTFTDLLSTVLMVVILILFSSESIS
                     GSVEQDLAKNVNASVEETFKKSGIPVKVDKSNGQVTFGEKTMFDVDSDVLKPEAKEML
                     KMFVPKYIETIYKDYGDYISKIVIEGHTDDVGSYIYNLDLSQRRAYSVAKFIVGDEIG
                     DYKYKDKVTKHIIAIGRSKAELIKNGDNSVNRDASRRVELKYEININQNK"
     gene            25206..26363
                     /locus_tag="PC471_001082"
     CDS             25206..26363
                     /locus_tag="PC471_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDF2788220.1"
                     /translation="MIEISIKNKKNSKIKTLILLLIVSISIYLTGCTTSDNGNNQSNN
                     NDSLSSSISSDKNVKIHFIDTGNSDAILIQDGKTFTLIDGGDNDDEGLMVDYLNNQGV
                     KDIKYLIATHSHADHLGGLDSVVKNFNIENVFVSNGSAETKSYRDFINALANKGLSPS
                     VPLENNKFYLEDSYFEVLNTNGGDTTNEQSLVLVYTNGNDKVLFTGDAEEGTEKEILP
                     KLEKVDLLKVAHHGSRSSSSQEFLDKVNPEYAVLLVGKGNSYGHPHQETMNKLKKMGV
                     KVHRSDECSDIIFESTGDGVFTSCKDGSYNKGVREDGNSGKSNSNTSKNDSFKNKQNT
                     SNSEEIVYFTPKGKSYHSTKNCSGLSRSKKILSGTIAESKKNDPCDICYGK"
     gene            complement(26510..27145)
                     /gene="lexA"
                     /locus_tag="PC471_001083"
     CDS             complement(26510..27145)
                     /gene="lexA"
                     /locus_tag="PC471_001083"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HDF2788221.1"
                     /translation="MYLDLTEKQVLILEFIKSQIILKGYPPAVREICTAVGLRSTSTV
                     HSHLNKLEKLGYIRKDPTKPRAIEVLERSKVNDVSGANQEIIELPLVGQITAGEPILA
                     QQNIEEYIPFPASLVKGSNNFVLRVKGESMINAGILDEDYVVVDKKNTALNSQIVVAL
                     INGESATVKRFFKEGNLIRLQPENDFMEPIMLNDSEVEIVGIVTGVFRVIK"
     gene            complement(27249..28376)
                     /locus_tag="PC471_001084"
     CDS             complement(27249..28376)
                     /locus_tag="PC471_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDF2788222.1"
                     /translation="MNNDKKIIKVHNKSDKPDNIVIKENELIEKCLLLENKLPVFMKD
                     YFIYLKGSVAVSTRLAYLEDINFFCSYLIETKELTNADCVKDITEDDFNTIKSRDINL
                     FLGDYCSRYYKNTEKNTLIFENNNRALARKKSSISTLFKFLYRNSQIDNNITDGFNPI
                     KLPKPQPDAIKRLEIDEVAKMLESVETGEGLTEKEKVYWRKTKLRDKAILALFVTYGL
                     RLNELRELNISSFNFSRGEFKIYRKRGKEVLMPINHTCEHVIKDYLQNERTRDELLND
                     EEKDALFLSLQNKRITAKAIRTLVKKYTSIPLDTTRDNGYSPHKLRATAATSLIQTGF
                     SIYDVQNLLDHDNVTTTQLYAAHKKNVKRDIVKNFEWIEDD"
     gene            complement(28687..29532)
                     /locus_tag="PC471_001085"
     CDS             complement(28687..29532)
                     /locus_tag="PC471_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2788223.1"
                     /translation="MIYIIEILKISVINIFCFIGIFIVFGLIFSITENLNNKFIFSSF
                     GKTGIIVTGAIGTFVHELSHLIMCLIFMHKINSVKFFRPIESKNDGILGYVSHSYKKD
                     NLYQSIGNFFIGIAPIIGGTIMIILIFKILLPDSYMKVTQNIDLKLYVSMINNLNING
                     FIHIILGDISNFIRIMLLTPSVYSLKYFIFMFLMYSISTHMSLSSADLKNSLNGLSFI
                     VIVIFVISSITYLFGFDNLNISSLIVKYNIFISFFMAIGFIFSIVTLIISYILSLVSP
                     FKNFS"
     gene            complement(29562..29933)
                     /locus_tag="PC471_001086"
     CDS             complement(29562..29933)
                     /locus_tag="PC471_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HDF2788224.1"
                     /translation="MKLKLLVEEYAVCRLNNDSKIPTWIDTEKFYSITRTDDELSVVC
                     LNNNIPSDVKSEKEWRILKILGPLDFSLVGILSKLSSLLADNKISIFAISTYDTDYIL
                     VKEKDIENACKILSCNGYEVE"
     gene            complement(30086..30346)
                     /locus_tag="PC471_001087"
     CDS             complement(30086..30346)
                     /locus_tag="PC471_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003866776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein S"
                     /protein_id="HDF2788225.1"
                     /translation="MEVLKVSSKSNPNSVAGALAGVLRERGVAEIQAIGAGALNQAIK
                     SIAIARGFVAPSGMDLVCIPAFTDIEIEGDKKTAIKLIIEPR"
     gene            complement(30535..31479)
                     /locus_tag="PC471_001088"
     CDS             complement(30535..31479)
                     /locus_tag="PC471_001088"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HDF2788226.1"
                     /translation="MIENNSEKIKVLENDIKQLITISKANNIDLSDKINSLNEKLEKL
                     KEDAFSHLSAYEKVTLSRDIKRPTTLEYIEHICSNFLELHGDRLFKDDPSMVGGIGQI
                     GKFNVTIVGHQKGRDTKENIKRNFGMPHPEGYRKALRLMKQAEKFNRPIITFIDTSGA
                     FCGLEAEERGQGEAIARNLLEMSKLSVPVITFVIGEGGSGGALGIGVGNDVCMLEHSV
                     YSVISPEGLSSILFKDSSKAKEACDVMKLTSNDLYDLKIIDKIIKEPLGGAQKDVEAV
                     SKEIKAYILERLNHYKDMDKEEIIAQRYKKFRNIGKCL"
     gene            complement(31500..32354)
                     /locus_tag="PC471_001089"
     CDS             complement(31500..32354)
                     /locus_tag="PC471_001089"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HDF2788227.1"
                     /translation="MIKKFLSSKESKYVTISLDDNFKKNSVDDKFWTYCKGCDSHVFR
                     KDIEENSFVCPKCSRHYGLRARKRINLLIDKGTFMEFNSDVEFQNPLNFPKYKEKVDS
                     YKEKTKESEAVVTGYGRINGIKTVICVMNPDFMMGSMGSIVGEKITYSIEYAAENNLP
                     IIICSASGGARMQEGMVSLMQMAKTSQALSKLEEKSLPYISVLTDPTTGGVTASFAML
                     GDIIISEPNTLIGFAGPRVIEQTINQKLPEGFQTSEFLLEKGFIDMIVDRRKMKEVLY
                     QILAMHKK"
     gene            complement(32369..33730)
                     /gene="accC"
                     /locus_tag="PC471_001090"
     CDS             complement(32369..33730)
                     /gene="accC"
                     /locus_tag="PC471_001090"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HDF2788228.1"
                     /translation="MIKKILVANRGDIAVRIIRTCKELGIKTVAIYSEIDKDCFHRYI
                     ADESICIGPNNISKSYNNIENIIYLALKLKCDAIHPGFGFLSENPEFAKQCEDNNIIF
                     IGPTREQMILMGDKSRARETMMELNIPVVPGSESVLKTKEEALEVAREIGYPVMIKAS
                     SGGGGKGMRIVRKEEELFSNFDMASSEALAAFSNSDLYMEKFIENPRHIEVQVFGDKH
                     SNAIHLGDRDCSMQRRNQKVIEESLSPYLSDEERQKLHKIAVDIVKGVGYIGAGTIEF
                     IVDKDKNFYFIEMNTRIQVEHPVTEMVTNLDLIKLQISIANGDKIPFKQEDITFRGHA
                     IECRINAEDSSKNFAPSPGKIESLNLPGGFGVRFDTFVYAGYTIPPLYDSMIGKLICW
                     AETREECISRIYRALDEIIVEGINTNVEFQKALVTSEEFREDTHHTKFIEDVFMKKEF
                     ATL"
     gene            complement(33746..34198)
                     /gene="accB"
                     /locus_tag="PC471_001091"
     CDS             complement(33746..34198)
                     /gene="accB"
                     /locus_tag="PC471_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HDF2788229.1"
                     /translation="MNINEIKELLKAIDSTNLEYVKLESSDLRLEVSKKAQSTSSPVL
                     SVQQESVVDLSLEKPVVNDTPVTSNENLSVVVAPLMGTFYDSPSPDADSFVKVGDVVE
                     EGDTLCILEAMKLMNEITSEIKGEIIEVLVSNEELVEYNQPLFKIKPL"
     gene            complement(34468..34914)
                     /locus_tag="PC471_001092"
     CDS             complement(34468..34914)
                     /locus_tag="PC471_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2788230.1"
                     /translation="MESKFFNWYTQSLGGTFGIISCICAYLNGYMFVYTNSASKYFES
                     IGFLGIISSYTLLPLCMLTLIFGVIYSYTPETSDKSIFNIPFFTLNKRLILITVVFGF
                     IGARVYFIIPAILITFNLYSNVLIAKLKYILCKNKENDTNLKESEV"
     gene            35189..35467
                     /locus_tag="PC471_001093"
     CDS             35189..35467
                     /locus_tag="PC471_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2788231.1"
                     /translation="MVDTLEFGLKILFFILSIIWMGKIMILRTDKQIVINPLLIGISA
                     ILVMLHTSQSNIEFFGLDVQYIRIVLYIIYSLIILIGIWATNRRNGIF"
     gene            complement(35596..36342)
                     /locus_tag="PC471_001094"
     CDS             complement(35596..36342)
                     /locus_tag="PC471_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2788232.1"
                     /translation="MWEGTMNLANLIPFIISAAALSPINSNDKVLSENQLKDIFPDAN
                     LRAVVKRYINPDEMTISNIKALDGEFYATGESISNLKGISYLENVDNFIFWNNNIKEV
                     PKEALSLKDMDSINLANNYLIDDDVVNSLSHNGVDVNCDLNFIDTKDNQYKLDSKYHN
                     VDIAKGESIDLRKIITKKIDSYYKYWEVTDNLPKDLNFIVSVSDKSVLSCEDMIIKGN
                     KTGKAVVKVILSDKNDLNTSQEVLITVNVK"
     gene            complement(36471..37115)
                     /gene="trmB"
                     /locus_tag="PC471_001095"
     CDS             complement(36471..37115)
                     /gene="trmB"
                     /locus_tag="PC471_001095"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HDF2788233.1"
                     /translation="MRRRRKKGADEKLLSYTKYVLRDDIDKLKGKWNLKFRNDNPIHV
                     EFGTGKGKFITTLAKQNPDINYIAMELKEEVLLKAVEKADASNLNNILFLWGDVSNIL
                     DYFEAKELSRIYINFCDPWPKNRWSKRRLTHSGFLEMYNRVLEDDGEIHFKTDNEKLF
                     EFSLNEIAANNWLLKNISLDLGNSEYENNVTTEYEDKFMSQGMRIFRCEAKKRN"
     gene            complement(37253..37573)
                     /locus_tag="PC471_001096"
     CDS             complement(37253..37573)
                     /locus_tag="PC471_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein HesB"
                     /protein_id="HDF2788234.1"
                     /translation="MKITLPETAIDTLKSILKDNQDKPNNIRVYFAGVGCGGPSFGLA
                     LDEKKEDDLTYEVGELQFVMSSDEYSQYGDIIIEDTGFGFRVIPENMKDQGGGGCSGC
                     SGCH"
     gene            complement(38026..38274)
                     /locus_tag="PC471_001097"
     CDS             complement(38026..38274)
                     /locus_tag="PC471_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDF2788235.1"
                     /translation="MQKTKAWGIIEPEKPESQQQMAHMDFAVKDLEEAVQYAIHCGAT
                     IAEEQFTDDWRVMIDPAGHPFCLCQMKSIMESSHFSLL"
     gene            complement(38305..38766)
                     /locus_tag="PC471_001098"
     CDS             complement(38305..38766)
                     /locus_tag="PC471_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase/bleomycin resistance/dioxygenase
                     family protein"
                     /protein_id="HDF2788236.1"
                     /translation="MKFGGVLLIVKDINKSKKFFEDVMEQKITLDLGEHVSFENGFGL
                     QSNYKAIIGYDLPIYQQSNNFQLYFEVNDIKQWESKINRIGNIEFLHYIKEYPWGQRT
                     FRFYDFDKNIIEISESMESVIKRLLKQGLALEEISKRTMYPVEFITQFQQV"
     gene            complement(38804..39637)
                     /locus_tag="PC471_001099"
     CDS             complement(38804..39637)
                     /locus_tag="PC471_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2788237.1"
                     /translation="MLKKESIHFINKNRSLIVCEDNFVYLLPCIQLRNWISNFTISFP
                     NQTIISDNYTIIPHGSVTLVFFYDATGLHSLLFGPTTKPKTVGNIANRCDVILIIEFQ
                     PAGFFPLIGIQQSELIDKVVPFSIINTSLDLEIKKIFNESLSIDKLILKLEELLISNI
                     KIEYSYEFILAIQLIIQNSGNISSQEISKKVFYSSRHLNRLFNQYLGLSMKSFSRLVR
                     INKSIKLLNNNKTSWMSICNELGFYDIPHFIKDFKIVCGITPQKYRANMSDFYSEIAK
                     F"
     gene            complement(39911..40597)
                     /locus_tag="PC471_001100"
     CDS             complement(39911..40597)
                     /locus_tag="PC471_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2788238.1"
                     /translation="MKITHNSLAVEAKNIIKEYKIGNTTTRVLKEVSLQVMKGEFVSI
                     MGQSGSGKSTLLYILGGLDTPTSGKVYMNGADISHFNDEKMSIIRRRNIGFVFQFYNL
                     IPNLNVEENIMLPLLLDGKNLKDYKNQLDEILDIVGLTDRRKHTPRELSGGQQQRVAI
                     ARALIGKPEILFADEPTGNLDSKTGIEIIDLLNKINRDNGQTIIMVTHSPEAAKSSSR
                     TITVSDGLIV"
     gene            complement(40612..43089)
                     /locus_tag="PC471_001101"
     CDS             complement(40612..43089)
                     /locus_tag="PC471_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HDF2788239.1"
                     /translation="MKIILKYIFTNIKERKIRTAVMLLSIVLSTVLLFVSFSIGLSYE
                     SAQRKMAKGMYGTATISVQSKNPDILTNLEDIPDLNTIKSKVGVLESSAIYNKGGYYE
                     EFSLISADLSQLNKINKPRLENGDSITDFSGDKIILPNRFTSKYKIKKGDSITLQIYG
                     KSYTFQVSDIASYDTVFLRNTRGVNALLPKETLSKIINKGSGYTRVLIESEEEITENL
                     VNKLSEELSTEKYMVSNTINETKIISDARQKTMPFFLISFFALTLSIFIIYSSYKVIT
                     LERLPFIGTFRSIGANEKTVTRILMLESILYGSIGGLIAIPIGVVVLNLMLHGLGNSL
                     EQGISIPVVISPIGVIISVIVAIIVSSFSAYIPVKKASHLPIKNIVLGTVEEKNVSNR
                     SILFIGSIMFILSILLPRISPENTLYLAGGFSLLGLIVATIVLIPLITDIMSIVFEFV
                     YKNILGNEGKLAARNMKNNKNIIQNITLLFISISAVIAISVVGNFVKTYITDVFRDAE
                     LQGFADGNMNEEFIEDVRHMEGIKKILPLYVMNNEISGNGVTLSRLEGTDNIKLYNSM
                     FGINYTNFEIKKQVIEAFNDKRSVILNEDTLKKVGLSIGDTITLSNDKYDFSYKIVGS
                     FKSRANDVEAVIPSHYAVNDFDKTNYGFLVYTAVNPDAVMIQIRYLFGDTYNWSRTVE
                     EFNNDSLNTISSFLSPMNKMTYFIFLLATVGIINNLLINYIQKRRSIAMYKSIGLSNK
                     QNIKVTLIEGVTSGLLGAVIGIVISILEIQTIFIVAGPKISMKPDLDFKTFIIVGLLG
                     IIVTLIGSIVPIIKGKKMKLIEEIKFE"
     gene            complement(43308..44711)
                     /locus_tag="PC471_001102"
     CDS             complement(43308..44711)
                     /locus_tag="PC471_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF2788240.1"
                     /translation="MHKPSYKTVFHSYIIFFMIFICLILTVIGIFLSLITIQKPNGEF
                     IRSDWPKKFTENFNEQIVFIDNKPQVKQSGLEKLRENQLWLQILDSSGNRVYGFLEPK
                     EHKDYYSNSELLALANQNNSSNGTVYFNTIKNNKNDYIYIIYFPLNISKVNMFFDGGK
                     FIGGKSIVFSIIGIIFLIILVSGIAYGYWVTKCISNITSAVSDISKRSYLPVNIKGVF
                     ADIYNSLNILNEEIHKSDEIQNKTDIMREEWISNITHDLKTPLSPIKGYAELLTDSST
                     NYSPEKVYKYASIMLKNIEYTNTLINDLKLTYQLKNGIIPLNKNNYDLVRFLKELSID
                     ILNYPEYESNIVEFKSNIDNINFDFDCTLLKRAFSNLIVNAFTYGDKNTKVCVHVTLN
                     ERIEIIISDNGKGMSREEASNLFNRYYRGSNTKQKIEGTGLGLAITKQIIEVHGGNIS
                     VESIPGLGSSFYITFPI"
BASE COUNT        16937 a         5959 c         6744 g        15152 t
ORIGIN      
        1 catagattat taatataaaa tcataggtta gtgtgaaaaa gttgaaattt aaatatttta
       61 aaagataatt tttttgaact aataggtata acaaaaattt atagggggat tgcatatgga
      121 aatcaatgtt atagaaattt tccctaaaga taaagctaaa cttaataaaa tagaaatgga
      181 taaagctagt tggtttgtaa atataataag taaaaaatat cctaaagaag ctttaaatga
      241 agcatttagt actttagaaa aagaattaaa tataagtaaa gctaatacat aagctttgat
      301 tattgatttt atatactatt ggttttatac gctatgactt gacattatat taatcaaata
      361 gtatattttt aaatatagca caatgaagtg ttaaaagagc aatggagctc catagataaa
      421 gtctatggag cttttttgcg tatttaactt aaggaggata ggactttgaa taactttgag
      481 attaaaacta gaataaaatt tggagaaggc tcactaaaag ctttgcaaga attaaaaaac
      541 aaaaatgtac taataataac agaccctttt atggtgaaat caaaaacaat agataaaata
      601 acatctaatt taataaactc aacatatcaa gtttttgaag aaatagtacc agacccacca
      661 atagaagtag tagtagcagg aatagaagca tttaaatcta taaatccaga cgtaataata
      721 gcattaggtg gtggttctgc aatagatgca gcaaaatcta taatggactt ttctaaaaaa
      781 atattaaaaa ttaacgaagt agagtttata gcaataccaa caacaagtgg aacaggttca
      841 gaagtaacat catttgctgt aataacagat agtcaaaaaa aatgtaagta tccattagtg
      901 agtgatgatt tacttccaga tatggcaata ctagacccag aacttgtaaa aactgtacca
      961 aattttataa cagcagatac agggatggat gtattgacac atgctataga agcttacgta
     1021 tctacaaatt ctaatgactt ttcagatgca ctagctgaaa aagctataaa gttagtattt
     1081 aaatacttaa taaaagcata taaagatgga aatgacatag aagctagaga aaaaatgcac
     1141 aatgcatctt gtttagcagg tatggcattt aaccaagcat cacttggcat aaatcattct
     1201 attgcacatg tattaggtgg aaaattccat ataccacatg gaagaacaaa ctctatatta
     1261 cttcctcatg taatagaata taattcatca atagtaggat atactaacac aacttactct
     1321 gctacagcta aaaaatatgc acaaatagca gaattagtag gacttgggag tgctaatata
     1381 agaatatctg ttagaaattt agtaaatgaa ataaagaaac tacaaaaaga aatgaatatg
     1441 ccaactaaat tgacacaatg caaattaagt ttagaagata taactaatga agaaagagaa
     1501 atagcaaaac ttgctataaa agatacttgt acattaacta atcctaaggt accaactgaa
     1561 gatgatataa taaacatatt aaagaacatt aaataactac attttattac atgtaaatat
     1621 aatattatac aaatagggaa aatgttttta aaaatataaa ttatatattg acaaatataa
     1681 aacataaaac tataatgaaa gtatcacaaa ggcgtgatac tttagtagaa agcaatggag
     1741 cttcttagga atattaatat tattcttatg gagctccttt tattttaatc aaatagcttt
     1801 ttaataaact atattcaaag aaagcaggga ataaatttga ctgaagaatc taagcaaaga
     1861 gttattcaag aatatgttcc tggaaaacag gttacattag cacatatcat agctaaccct
     1921 aatgaagata tatataagaa gttaggatta gtacttgata aaaaagatgc aataggaata
     1981 cttacaataa caccaagtga agcgtcaata atagcagcag atgttgctac aaaggcttca
     2041 aatgtatctt taggttttat agatagattt agtggttcag tagtaatatc aggtgatgta
     2101 agttctgtag aatcagcgtt aaatgatgtt ttagaagtct taggaaatat gttaaacttc
     2161 tcatctacaa aaatcacaag gacataataa tacaatgaag aatataatat ttatgggaaa
     2221 gacaggtagt ggtaaaacta ccttatgtca aaaactagac cagttagaat taaagtataa
     2281 aaaaactcaa tcagtggaac tttataataa tgcaatagac actcctggtg agtatatgga
     2341 aaatagaaac ctatataatg ccttaataac aacatcagca gatgctaaaa ttatagcaat
     2401 agtgtatgat gccacacaag aagaaaatta tatagcacca ggttttgcca gtatgttttg
     2461 caaagacgta ataggaatta taactaaaat aaataaagct actgaaaacg aagtcaatat
     2521 agcttatgaa agactatcac tagcaggagc aagtcaaatc tttaaaatag acacagttga
     2581 taatattgga cttgaaaaat tatttaaata tctagaaaaa tataaaaata tagatgaagt
     2641 ttaaggggaa atattttttt attcaaaaat attattaaag tatgataaaa taaaaaaatg
     2701 tataaactat tttaagaaga tttaatcttt aagcgaggta ggaacatggg gaaaaaaata
     2761 ctattggttg atgatgaacc actgttaagg atggatacaa aagatatatt agaaagtcat
     2821 ggctatgaaa taataggcga agcaagtgat ggatttgaag ctgttgaaat gtgtaaaaaa
     2881 cacaaacctg atttagttat aatggatata gagatgccaa tacttgatgg tataaaggca
     2941 ggcaaaataa tatgtaaaga aaatatagct ggtggagtac ttttattaac ttcatttgaa
     3001 gataaagaat acgtagaaaa ggctaaaagt ataggtgctt atggatattt agtaaaaaca
     3061 gcaagtgaaa aatcacttat cccaaccata gaaatgtgtt taaataaggt aaatgaattt
     3121 gaaaaaatga aaaaagattt agaaaaagta aattctaaat tgaatgaaag aaaattagta
     3181 gaaagagcaa aaggtatact tataagagag tttaagattt ctgaggatga agcatacact
     3241 agaataagaa aattaagtat ggataaaaga actacaatga tggaaattgc caaaatgatt
     3301 ataataggat acgatgacta atacgataaa aaaaatatca actgcacaaa catacttaaa
     3361 taataacgaa attgaaaaaa ttatagaagt atcaaaatcc ttagatttaa tggcttcttt
     3421 ttatgaagct gatgttttta tagatgtact aagtaaattt gatgatgaag caattgttgt
     3481 ggcacatggt agacctaaag aaaagtcaat gtactgtgaa aatgttatag gtaaaaaggc
     3541 tttaattgaa aatgaaccag gagttataaa gtgcttaaaa acaggagaaa gagtaagaga
     3601 tattaaagct ttaacacagg aatataaact ggtcaaacaa aatattcagc caataacact
     3661 agatgacaaa gttttagcag tactaatagt tgaaaaagat ataagtagtg aagttaagaa
     3721 tgagtttaat gctagtgaag acgaagaaaa taaaaattta catcagttta taaaacttat
     3781 tggaaataat gactttttag ttgataattt aaattcagcc atattaatat ttgataaaaa
     3841 tggaaaatta aaaattaaaa atcgtattgc aactgagata tacaaaaatc ttgggtttgg
     3901 atatgatata caagatatgc attacaatga tttagatata gaaaccaaca cctttgaaaa
     3961 tatattaatg gatgaaaatt atgatgagac aaatgaagtt tcaataggta atatgttttt
     4021 taaaataaag accatataca tcaaagatga tgaatttaag gtttgcaaaa tagtacaaga
     4081 tataagtgat ttaaagagaa aagaagaaga attagttttg aaaacagtgg ctataagaga
     4141 agctcatcat agagtaaaaa ataatcttca tacagtaata tctataataa aaaaacaaag
     4201 tagattatct caaaatgatg aagtaaagca gtgtttagat aacataataa atagggtatt
     4261 tgcaatactt tcgactcatt atttactatc taaagaagtt gataataata tatctatacg
     4321 agaaggtatc aatttattag tgtcaaatat tcaaggtggc tatttattta gtaaggatat
     4381 agatatacgt ataactggtg aagattttaa aattagtgga gaaaaagcaa cagcaatact
     4441 tttggttata aatgaaatac tacaaaattg ttatgaccat gcattttcgg atagagaaca
     4501 tggtactata caaatattaa taaataaaga taacgactat agaaatattg ctattataga
     4561 tgatggaatt ggatttgata gtaataatat aaataaaaat agcttaggga catatataat
     4621 agatagttat ataacacaga tgttaaaagg aaatatagag agaaaaagta gtgagaatgg
     4681 tacagaagta ttaattacta tacctacaca aattaatatt taaataataa ctcatctaca
     4741 aagtagtagt tttaattgag caaagaggct taaggttatt aatagattaa gttaattgaa
     4801 tgtatttata aaaattataa gtatatattc aatttaacag gtatttatta taactttaag
     4861 cctctttgct tgtttaagac atacttaaat tttaggaggt gagaaaatga gagaagagtt
     4921 attaagtgtt ggtattgaca ttggaacaag tactactcag ttaatttttt ctaaattaat
     4981 agtagaaaat actgaatcaa atttttcggt acctagaata agtatagtag acaaagaaat
     5041 tatttataaa agcaaagttt atttcacccc tcttatttca aataatgaaa taaatggtca
     5101 acaaataaaa gatatagttg aaaatgaata taaaaaagca aatatatcta aagaagatat
     5161 acatacagga gcagtaataa taactggtga aacagctaga aaagaaaatg caaatgatgt
     5221 acttcatact ttgagtggat ttgctgggga cttcgttgtt gcaacagcag gacctgattt
     5281 agaaagtata atatcaggaa aaggagctgg agctcatatt tattcaaaag aacattcaac
     5341 tacaattgta aatatagaca taggtggggg aactagtaac atagcctcat ttaaaagggg
     5401 agaagtaacc gatactgggt gtttggatat aggtggaagg cttgtaaaga tagataaaat
     5461 aagtaaaaaa ataacttata tatcacctaa aatagataaa atcataaaag ataataattt
     5521 aaatcttcaa gtaggcacaa tggctactgt agaaaataca aagccaatag tggatataat
     5581 ggttgactta ttaatggaaa gcataggatt aaagaaacag acacctttgt ttgattttat
     5641 aataaccaat aaatcaataa gtatagatga aaatattaaa aatgtttcat tctctggtgg
     5701 agtagctgat tatatttact atgatggaga aataaaagat tattttgaat atggtgactt
     5761 gggaatttta cttgggcaag caataaaaaa ttgtgaatac cttaaaaaag taaatgtagt
     5821 taaatctatg gaaacaataa gagccacagt ggttggagca ggttctcata ctactgaagt
     5881 aagtggaagt acaataactt acactagcga aaagtttcca cttaagaact tgccagtttt
     5941 aaaattatca attgatgaag aaacaggcac ttcaaaacaa atcagtgaag ctataaaaaa
     6001 taaacttaat tggtttaaac tagaaaatga tttacagaaa gttgctatag ccatgatagg
     6061 aaagaaaaat ccaacctttt tagatataca agagtatgct aaagggtttg tagatggaat
     6121 gcaacaaatc atagaaaatg atttaccttt tatagtaatt gttgaaaatg atatggcaaa
     6181 agtgttaggt caagcaatat atgcattatt aaattatgaa aaagaaattg tttgtataga
     6241 cagtgtcaag gttgaaaatg gagactatat cgacattgga aaacctatag ttgaaggtaa
     6301 agttttacct gtggtaataa aaacattagt ttttaattaa tagaaggagg taagtaaatg
     6361 attttaaata caaaattatt tggacgtact tatcagttta aaggcttata tgatgtaatg
     6421 gctaaagcta atgaagaaaa atcaggagat aaattagcag gtcttgcagc agaatcagct
     6481 gaagaaagaa tagcagcaaa agttgtttta tcacacataa aattggagga tataagaaat
     6541 aatcctgcag tggcatatga agaagatgaa gtaacaagaa taatacaaga tgctgtaaat
     6601 gaaaaagttt atgaagaaat aaaaaattgg acagtctctg aacttagaga atggctattg
     6661 gattcaaata ctacaagtct tgatataaga agaatatcaa gaggattaac ttctgaaatg
     6721 atagcagcag ttgcaaaatt aatgtcaaat atggacttag tttatgcagc taaaaagata
     6781 aaggttactg ctcactgtaa tacaactata ggagaacaag gaacattatc agcaagactt
     6841 caacctaatc atccaacaga cgacccagat ggaatacttg catcacttct tgaaggttta
     6901 acttttggta taggagatgc agtacttgga ttaaatccag ttgatgactc tgttgagagt
     6961 gttactaaag tattaaaaag atttgaagaa gtaaaacaaa aatatcaaat accaactcaa
     7021 acatgtgtat tggcacacgt tactacacaa atggaagcaa taagaaatgg tgctcctgca
     7081 gatttaatat tccaatctat agcaggttct gaaaaaggaa atgaagcatt tggatttaat
     7141 ggcgacacta tagaagaagc tagacagtta gccttaaaac acggtacagc tacaggacct
     7201 aacgttatgt attttgaaac tggacaaggt tcagaattat cttcagaagc acatcatgga
     7261 atagaccaag ttactatgga agctagatgc tatggatttg ctaaaaagta tgaaccattc
     7321 ttagtaaata cagttgttgg gtttatagga cctgagtact tatatgatgc tagagaagtt
     7381 gtaagagcag gacttgaaga ccactttatg ggtaaactac atggcttacc aatgggtgtt
     7441 gatgtttgtt acactaacca tatgaaggct gaccaaaatg atatggaaaa cttagccata
     7501 ctattaacaa cagcaggatg tacgtatttt atgggaatac ctcatggaga tgatgttatg
     7561 cttaactatc aaacaacagg ataccatgaa acagcatcac ttagagaaat gtttggacta
     7621 acagcaataa aagaatttga tgcttggatg gaaaagatgg gattctctaa agatggtaag
     7681 ttaacagagt tggcgggaga cgcatctgta ttattaggat aggaggtatt taatatgaat
     7741 gagaaggatt taaaagcatt ggtagaacaa ttagttggac aaatggttgg agaattagat
     7801 actaatgttg tgtctgagac tgttaaaaag gcaactgaag ttgtagtaga caacaatgct
     7861 tgtatagatg atataacaga agttgatata agaaagcaat tactagtaaa aaatcctaag
     7921 gatgcagaag cttatttaga tatgaaagca aaaacacctg caagattagg tataggaaga
     7981 gctggtacta gatataaaac agaaacagtt ttaagattta gagcagacca tgctgctgca
     8041 caagatgctg tattctctta tgtagatgaa gaatttataa aagaaaataa tatgtttgca
     8101 gtggaaactt tatgtaaaga taaagatgaa tacttaacaa gaccagattt gggaagaaaa
     8161 ttttctccag aaactataaa taatataaaa tctaaatttg gtacaaatca aaaggtttta
     8221 atattagttg gagatgggct tagctcagct gcaatagagg caaatttaaa agactgtgtg
     8281 ccagctataa aacaggggct aaaaatgtat ggtatagatt caagtgaaat tttatttgtt
     8341 aaacattgta gagttggtgc tatggaccac ttaggtgaag aattaggttg tgaagttata
     8401 tgtatgttgg ttggagaaag accaggatta gttactgctg aatcaatgtc agcatatata
     8461 gcttataagc catatatagg aatggcagaa gcaaaaagaa cagttatatc aaatattcac
     8521 aaaggtggaa caactgcagt tgaagcaggt gctcatatag ctgaacttat aaaaactatg
     8581 ttagataaaa aagcttcagg aatagacctt aaataaagtc gagggagtgt agaatatgaa
     8641 aaatgatgta attcgtccta atatactagg aattaaaata atttcaaata taagtccaga
     8701 aatggcacaa aaactagaat taaaatcaca tcacaaaagc ttaggtttaa taactgcaga
     8761 ctgtgatgat gttacgtata cagctcttga cgaagcaact aaagcttcag aagttgatgt
     8821 tgtatatgca aaatcaatgt atgcaggtgc tgcaaatgct tcaacaaaac ttgctggtga
     8881 agtattagga ataatagcag gtccaagccc agcagaagta agaagtggtc taaatgcagt
     8941 agttgatttt ctagagtatg gagcaacttt tataagtgca aatgatgatg attcaatagc
     9001 atactatgct cactgcgtat caagaacagg tacatacctt tcagaggtag caggaataag
     9061 agaaggtgaa gcattagctt atttagtagc accaccactt gaagcaatgt atgcactaga
     9121 tgcagcaatg aaagctgcag atgttaagat gtgtgaatta tttgcacctc caactgaaac
     9181 aaactttggt ggagcattac ttacaggttc tcaatcggct tgtaaagcag catgtgatgc
     9241 atttgcagaa gcagtaaaat cagttgcaga caatccaaca ggattttaag gatagttaga
     9301 tggatgctat aggcttaata gaagtaatag gatatgttgc tgcaatagaa gcttctgatg
     9361 cttgtttaaa atctgcaaat gttaatattg taagagttga taaggttgga gcaggtattg
     9421 ttactttaac aatatgtgga gatgtcggtg ctgtaaaagc tgctttagaa gcaggtgaaa
     9481 tagcagcttc cagagttggt acattaagaa catcacatat tataccgaga atccataatg
     9541 aagtaacaaa tgctttattc aaaactaaag aacctaaagt ctgtgaagtt tcagaagata
     9601 aagaaaaaaa ctttgagatt acattggaaa tagctgataa tgtatcgaaa aatttatcag
     9661 aaaaagaaaa tgtaatttta gatgtcaatg aaaacagtgg agatactgaa aatttatata
     9721 gtgatataga taaaacaaac aaaaaaattg aaacagttga tgatagttta acagaagcta
     9781 atgaggaaaa aatagaaaac actaatgata aagaagtatt ggaacaagtt aaaatagaaa
     9841 attccgattt tgatagagat gacctatcaa agaacagtgt aaaaaaactt aaagctatgg
     9901 ctaagaaatt agattcttca ataacttata aagacttaaa tgttcttaaa aaagaagaat
     9961 taataaactt agttaaaaag cttagtagaa gggatagata gttatgacac ttttagataa
    10021 agatttagta tctatacaag aagtaagaac tcttgtaaga agatctaaag tggctcaaga
    10081 gaaactttct aaaatgagtc aagagcaaat agataaaata gtaaaagctg ttgtagatgc
    10141 agcatttaat aatgctacta aattagctaa aatggcaaat gaagaaactg gatttggaaa
    10201 atgggaagat aaagtaatta aaaatacttt tgcatctatg ggagtttatg aaactttaaa
    10261 gaacacaaaa actattggaa taattggtga agataaagaa gccaatacga tagatatagc
    10321 tacaccagtt ggagttgttg caggtcttat accttcaaca aacccaacat caacagtaat
    10381 atacaaaact ataatatctc taaaagcagg aaatactata gttttctctc cacatccaaa
    10441 tgcaaaaaac tgcatacttg aaacagttaa tataataagt gatgctgcta taaaggctgg
    10501 agctccagag gattcaatat tatgtataac taatccaagt atagttggaa ccaatgaatt
    10561 gatgaaacat aaagatacaa acttaatact agcaactgga ggagaagcaa tggttagagc
    10621 tgcttactct tcaggaacac cagctattgg agttggacca ggaaatggac cagcatttat
    10681 agataaatca gcaaatgtta aattagcaat caaaagaata atagattcta aaacttttga
    10741 taatggtgtt atatgtgcat cagaacaatc tatagtaata gaaaaatcta tggaagaaac
    10801 tgttttaaaa gagcttaaaa atcaaggtgc atatgtttta aatgaggagg aaagtgaaag
    10861 attagcaaaa ttcatattaa gagctaatgg tactatgaat ccacaaatag ttggtaaaag
    10921 tgttgaggta atagcaaatt tagcagactt aacaaatgta cctaaaaata caaaagttct
    10981 agttgcaaga gaaaataaag ttggtcacaa tgtagcctat tcaagagaaa aacttacacc
    11041 aatactagcg ttctatgttg aagaaaatgt agaagctatt atgaacagat gcagagaaat
    11101 attgttaaat gaaggtgcag gtcatacatt ctcaatgcat gtaaatgatg aagaattggt
    11161 aaaaagattt gcccttaata tgccagtatc aagaatactt gtgaatactc cttcagcact
    11221 tggaggaata ggtgcatcaa caaaccttgt accagcacta acacttggtt gtggtgcaat
    11281 aggtggaagt tcaacttcaa acaatatagg accacttgat ttaataaatg ttaaaagagt
    11341 agcttatgga atcaaagaaa tagaagaact tggaagagat aaatctcaag ttgaaaaatc
    11401 aatgtttgac tcaatggata aagaagaatt aataaatctt cttgttcaaa aactagtcaa
    11461 agaactagca taactaaaat aaacaatgaa acttatggct atcgctataa atttctaaaa
    11521 atataaacta taaaaaacta taaaattaaa tttttggagg ataagattat ggcaagtgca
    11581 aacgcattag gaatgataga aactaaagga ttagtaggag caatagaagc agcagatgca
    11641 atggttaaag ctgcaaatgt acaattagta ggaaaagagc aagtaggtgg aggattagtt
    11701 actgtaatgg taagaggaga tgttggagct gttaaagctg ctacagatgc aggagctgcc
    11761 gctgccgaga gagttggaga actaatatca gttcatgtta taccaagacc acactttgaa
    11821 gtagatgcta tattaccaaa ggtatcagct gagtaatata agaataatta taaaatttaa
    11881 attttgattg aaaaagaggc tatctcaaaa atgagtctta ctaataaact caatgtattt
    11941 tgaggtagtc ttttttatac atctaatagg agatgtgata aaatgggtgt tttaacagaa
    12001 aacgaagtaa aaagaaaaat tggaaaaaac aaaaataata taactgaatt ttatatagaa
    12061 aaaggtcaaa taataactcc ttctgcaaaa tcttatttag ctgagagaag tattgagctt
    12121 aaatatgttg ataaactttc agatgtaaac aatgaaccta aagaagttgt aaaagaagta
    12181 gttaaagaga aaaaatatag atatactact gtatttggcg ctaaattgga tgaaaaacca
    12241 gaacatatga ctcaattaaa tggaaatctt ttagttttta aagaccataa aagaataata
    12301 ttgagaggaa aaatagattc tcttgaatca gaaatactaa aggtacagat actttgtcag
    12361 aaactagata tgccaaagct tgttaaggaa ttgcaagaag tattagactt tgtaagaaat
    12421 ataatgagat gtgaagtttt agatgaaaag ctaggggact ttaaactttt aggattaact
    12481 cctcaagaac ttagagaaca atctcactat cctaaaaaat actttggtat aggacatgag
    12541 ttcccaagtt atgaaatggg agaagtagta gtagctataa atagtataag aagtaataca
    12601 cgagaggttg aacttgcagc ctatgaagca tttaagggtg agtatggaaa tgttcacaga
    12661 gaagatttaa taaaggcact taatagatta tcgtcagtat tttggataat gatatataag
    12721 gttagaacta ataagtataa gtagttggag gacataatgg agaatctatt aaatgaaata
    12781 gtggaagaag taataactag agttaaaaaa gaagcattta tagaagttga agcatcagga
    12841 agacacgttc acttaagtag agaagatgtc gataaattat ttggagaagg atatactctt
    12901 acaaaactaa aagacctatc tcaaccaggt caatatgcat gtaaggaaag agttacaata
    12961 actggtccta aaggaagtat aaaaaatgta gttgtacttg gaccatgtag aaatgaaact
    13021 caggtagaaa tatctttaac agatggttca actcttggac taaaagctcc tataaaacaa
    13081 agtggagact tagaaggtac tttaagtatt aaaatatcaa cacaatatgg tgaagttgaa
    13141 cttgataagg ggttaatggt tgctaaaaga catatacaca tgactcctaa agatgctgaa
    13201 aaattcaatg tatgtgataa agagatagtt gaagctaagg tgatgggtca aagaccttta
    13261 acatttgatg atgtagtaat aagagtgagt gacagtttta aaacttatat gcatatagac
    13321 tatgatgaag ccaatgcatg tggttattca aaaggaactg ttgctaaaat aataaagaaa
    13381 gcgtaaattt aacctactat ataaattacc tctagggagg atttaatatg aattatgata
    13441 accttgtaga tttaataacg gaagaaatct ataaaaaaat aaatagtaat gaaataaaaa
    13501 tatctaataa acctaaagct gtgattgtat ttgaacaaga taacaataaa tttaacttat
    13561 taaaagatga atttgaaata gtagaatttg ataaaagtat aagggaatgt gaaatagtga
    13621 tagtttcaag actttgtatg agggggctat cgaatatagc acttggaaat tcaacaagtg
    13681 atgaagaaag atttatacta aaaatgatta tgaagggcaa agaagtatat gtcctagatg
    13741 aaggaattga atacaaaaaa tataaggata ctgcaccaaa agcattatat aagaaattta
    13801 tgtcatttga agatgaaata tgtaaatttg gtgtagaaat tataaaagac ttaaatgtta
    13861 taactaaaaa taagttaaat ataaagaaag aactagaaag tactagaggt tctaaaaaag
    13921 aagatacaaa agttttagat aaagatgttt taaatttaac cagtgtagaa agtgtaaaaa
    13981 tagataatga atttagttta gatttaagga ataaaaaact tatatcggaa gctgacttaa
    14041 gaaaaccaac tataaatgga gttaaaaaca tattagttaa taaaaaaagt ataataacac
    14101 cattagctgt agattttatg agaatacacc atctcaaact aaagaaagtg taaggaggta
    14161 attttatgga tataggaaaa gttgtaggta atgtttgggc tacaagaaaa gatgaaaaat
    14221 tatgtggcca aaaactttta gttgtaaaaa ttttaaaaac taaagacaca tacaaggaag
    14281 gcttcatggt tgcagccgat aatgcaggag caggaaatgg agacttagtt cttatatcca
    14341 aaggttcctc agctagagag tcgattgaaa aatctcatgt tccggtagat gcaactatag
    14401 ttggagtagt cgattcgttt gaggtgtttg atgaataaaa gtataggagc tatagagttt
    14461 aaaagcattg ctaaaggtat agaagtatcc aatgaaatga taaagaaatc ttcagttgat
    14521 gtattgtact taaaaagtat ttgcccaggt aaatttctaa taatagtagg tggagaaact
    14581 tcttatataa atgaatgtgt agattatgga ataaaacttg gagaaggata tattgtagat
    14641 aacttcgtaa taaatgcaat ttcacaagaa atattagatg gatttaaaaa taagtatcaa
    14701 aagctagata gtatagtctc tataggcgtt gttgaaagta gtaaagtatg cgctggaata
    14761 aaaatgcttg ataagacttt aaaatctggc gacttagtat tagtaaaact tcaattatct
    14821 tttgctatag gtggcaaatt agtatatata gtagcaggag atttaagtag tttagaatat
    14881 gcattaaaag aaagtgaaaa tgtagtaaga gaaaaagaag taatatataa gactgtaata
    14941 ccatcagtag acagtcaaat aataaaaagt ctaataaaat aatgggggga attatgagta
    15001 taaacgaaat tataatctat gtaatggtag tcttcatggt acttggagct atagataaat
    15061 gtataggtaa caaatttggt cttggagaac aatttgaaga aggtataatg gcaatggggt
    15121 cacttgcagt tgcaatggtt ggtgttatat gtttagcacc agttcttgct gatgtattaa
    15181 gaccaattgt agtaccagta tttaatatac ttggagctga cccagctatg tttgctggaa
    15241 gtttattagc taatgatatg ggtggtgcac cacttgcact tgaacttgcg aaagacccta
    15301 atgctgggtt atttggtgga ttaatagtag gggctatgat gggacctaca atagtattta
    15361 taataccagt tgcgcttgga ataattgaaa aagaagacca aaaattctta gcaacaggaa
    15421 tacttgcagg tataataaca atacctattg gagcattcgt tggtggatta gttgcaggat
    15481 tcgaggtaat gatggttctt agaaacttag tgccaataat aatatttgca gtagttatag
    15541 cacttggatt attaaaattc gaaaatgcta tgataaaagg attcacatac tttggaaaag
    15601 gtgtagtaat agttataact ttaggacttg ctgctgccat agttgaagct ttaacaggaa
    15661 tagttgtaat acctggaatg aaaccaatca gtgaaggtat agaaatagtt ggagatatag
    15721 caatagtttt agcaggagca ttcccattag tgtttgttat aacaaaggta tttaataaac
    15781 cattaatggc tttaggaaaa gttcttggaa tgaatgatgt atcagcagca ggccttgttg
    15841 caagtttagc taactgtata ccaatgtttg gtatgatgaa agatatggac aatagaggaa
    15901 aaataataaa tgtagcattc tctgtatcag cagcattcgt atttggagac cacttaggat
    15961 ttacggcagg atttaattct aatatgataa caccaatgat agttgctaaa ttagttggtg
    16021 gtataacagc agtattgtta gctatggtta tcgcaaataa gacactaaag aaggagaatg
    16081 tataagatgg atatatcaaa tatagataaa aatttaattg aaacattagt aagacaaata
    16141 atagaagaaa aaataagtgg aactaaagac acagtagatt ttgttagaaa taaagacatt
    16201 agtggaataa cttctataaa gttaccaact gtaaaagtta gtgaatctga tagattagat
    16261 acagggaatc caagtgatgt agtttacaca aaagatttat ttacattaga ggaaagccca
    16321 agattaggtt gtggtatgat ggaaatgaaa gaaacaactt ttgattggac tctaaactat
    16381 gatgaaatag attatgtaat agatggaact ttagatataa taatagatgg aagaaaggtt
    16441 tctgcttctt caggtgaatt aatatttata ccaaaaggaa gtaagataca attttctgta
    16501 cctgattatg ctagatttat atatgtaaca tatccggcag attgggcatc tcaaaattaa
    16561 taaaaatcaa gttaagttaa taataatcaa ataaataatt taaaatacaa actcttaaat
    16621 aagcacacat atttaaaaaa gctatgaatt aaaattcata gcttttttta taaagaatgt
    16681 tatattttag acgtattttt tctatattta ctgggagaca tattatattt tttattaaat
    16741 aatctaataa aatgctctac atttggataa ccaatactat tttactgata atcttttcct
    16801 tatcactaat cttacatttt aaaatattat ttttactcgt aatattatca taagaatttg
    16861 tttcttattt aaaactaatt agaattataa agtttattgc catgttatta ataaaataaa
    16921 tccagttaaa tcgagtgcta gttaaagtaa taagaaactt ttacaatggt ttcctttatc
    16981 gattgaaata aaagttaata tggtataata tctaaagaga gaatcttatc aattagaata
    17041 tgcatagaaa caagtggctt aaaaggagac gttatatgga agagatagta aagaaggaac
    17101 aagcaagtat gaatgatatt tttttgtatc ttaaagctaa atcaaactca ttatataaat
    17161 ttgtaatatt atataatgag tttatcaata aacctaaaga ttatggaact ggggaactta
    17221 taaacatggt tgaagtacat atgctaacag cgattgaaga aaatccagga ataagtccaa
    17281 ctcagttagc taaaatatgg aacagaacta aaggtgcaat atctcaaact atatctaaat
    17341 tggagaaaaa gggttatata actaaagaaa agaaagaagg aaatgcaaaa actatattac
    17401 tatatccaac cacatcagga gtagagttaa gtaaggcaca taaactatat gacaccatag
    17461 atgttacaag aacatttgaa tacttaatca aggagtgtac attagaggag atagatagtt
    17521 tttataaagt aattgatttg tttattaatt tattagaaga atctccgcag gaaaataatg
    17581 attctgaaaa agtgtagctg agattgaagt tgaataaaga gaaatagata ttatagaagt
    17641 agtacttaag tacacattat ataaaaatat aataggtata ggattaaaaa gtttagtggt
    17701 ttaaagttta accactaaac ttttttaatg tctataatct actattattt aatttactaa
    17761 aaataaaaaa actcattata ttaagataaa aatagaaaaa taactagaaa aatactataa
    17821 tatatcagct taatacagaa aaaagttaaa aaatttgaga aaaataaaaa tgaaaacgtt
    17881 ttatattgac atagagaaaa agtagtgata ccatttaaat atagtttagt agctaaacta
    17941 tatttaaatt ataataacta ttttaggggg atgttatgaa tttaaaacaa ttaagtgaaa
    18001 aatacgaaga atatgtaatt caaatgagaa gagaatttca taagtatcca gaattatctt
    18061 ttaaagaaat aagaacaacc caaagaataa gggaagaatt acaaaaaata ggggtccctt
    18121 ttgtatctat tgaaccaaat atcgtaatag ctactataca aggcaaaaat gaaggtaaga
    18181 caattgcaat tagaggagat atagatgcgc ttccaatgca agaagattca gatgttgaat
    18241 acaaatcaga atatgatgga ataatgcatt cttgtggaca tgatgctcat ggagctatgt
    18301 tactaggact tgcacatatg ctaaatgata taagaaaaga tttaaaaggt agagtattat
    18361 tagtttttca agcagctgaa gaagttggtg gtggacacaa ggagataata cagtacttta
    18421 aggaaaatgg aggaccagat agattaatag ctactcatgt ttggtccgat attgaagctg
    18481 gtaaaatttc tgttgaacca ggacctcgta tggctggtgg gtctggttgg agaatagaca
    18541 taacaggtag aggaggtcat ggttctagac cagatcaaag catagatcct ataaagccag
    18601 cttgtgatat agttttaaaa gtatcttcta taccagttaa tcatataagt gtactagaac
    18661 aatgtgttgt acatgcatgt gcaatacatg gaggaactac aataggaaat gttattccaa
    18721 acaatgcaga ggtaataggt ggatatagat attttactga agaatcaggc aaaaaagtgt
    18781 ttgacattat aaagcaaatg agtaatggtg ttggacaagt ttatggagtg gatgtgaagg
    18841 ttactcatac tggaggcata ggacctgtta tcaatgatga agaagcagtt aagatggcta
    18901 aagaaatagt ttctaatata gatggattgg agttagattc ttatgagcca atatgtgcat
    18961 cagattgtta tggtgaaata ttaaaagagt attctggctt ttattgttat ctaggggtag
    19021 gtaacaaaga aaaaggaata atatatgcac aacaccatcc taagtacaat atagatgaag
    19081 atacattaaa gttaggttgc gaatttatgg ctaaatatgc agttgaattt ttaaataaaa
    19141 ataataacta aaataaatcg aattttataa atggggggga tgaaaatgca aggtgcatta
    19201 aaggaaaata agaaaaaaag tggtggagga tttttaggat ggattgaaaa agttgggaat
    19261 aaaatgccac atcctctagc attatttaca tatattacat tgatagtagt agcattatct
    19321 gcaatagcaa gctttttagg tttttcagca acaagtcctg ctacaggaaa gttaataaaa
    19381 gttatgaact taataagtgc aaaaggttta gtactattta tgcaagagtt tgttaagaac
    19441 ttccaaaact tccctgtgct tggagtagtt ttagttatgg cagttgccac aggagtttgt
    19501 gaaaagacag gatttttttc aactgccata aaaatgagtt tatcaaaagt taaggggaat
    19561 gcggttgtat ttataatagc ttttatagga gtatttgcta atcaagctgg tgatgctgct
    19621 tttgtattag taccaactct tgcaggagca atattttatg gattaaatag aaatccatta
    19681 gctggtatat tctgtggatt tgcatcagtt ggtggtggat ttgcaacagc tgtaattcct
    19741 gggggatggg acgttacgct aacgccaata actgtatcag ctgctcaaac tattcaacct
    19801 gcatttgata tgacattatt agctagttat tatgcactat ttgtatctgc gtttatagta
    19861 gctacagtat caacaatagt aacggttaaa tttatagaac ctaaattagg taagtatact
    19921 ggtaaaccag aaggtataga tgataatcaa ggttttgagg tcacaaaaga acaagcaaaa
    19981 gcagcaaagt tagcaggttt tacagtttta gcttatgtag cgttattaat tgctttgtgt
    20041 ataccagcta atagtttttt aagaagtcca gaaggttctt taatatttgg agcaccactt
    20101 atgtcttgct tacagttttt tatagtaata ctattcttct tgccagggat tgtgtatgga
    20161 atgagggttg gtaaaatcaa gactgtaaaa gacttaaatg atatattatg tcaatcaatg
    20221 gcagcaatgg caccgtttat cgtacttgca atagttatag gtcaattctt aacgctattc
    20281 tcagtgtcta atttagcaca ggtactagct ataaaaggtg gagcattact caactcattg
    20341 ccactaccac cacaagctat aatactgcta ttcttagtac tagtagcgtt tataaatata
    20401 tttgttataa gcggaagtac aaaatggatg atatttggac cagtatttgt tccaatgtta
    20461 atgcaattaa acatacatcc agcatttact cagtttgtat atagattagg agattctgta
    20521 accaatcatt tatcaccatt aaatgcattc tttataatac ttttagcaac agtccaaaaa
    20581 tacgataaaa atgcaggtat gggaactata ttctcagcta tgatacctta cactataggt
    20641 tactttgtag tacttgcagt tttagtagta gtttggatga caataggtat accagtagga
    20701 ataggtggac aagtttggtt ataaaacata tttaataatt ttgggaggaa agaaaatgga
    20761 aacaactgaa ataagataca attttgatga agtaatagat agaagtggaa caaactgtct
    20821 taaatatgat cttaagagtc aatttatgcc agaatctcca gaagatgcac taccactatg
    20881 ggtagcagat atggattttg catgtgcccc tgagataatt aatgcaatgc atgaaagaat
    20941 agacagaaaa atatttggat atagctctca acaaactgat gattattatg aagctgtatg
    21001 tggatggttt aagagaagat ttgattggaa aatagaaaaa gagaatatat tctttagccc
    21061 aggggttgtg cctgcgttag gatttttcac acaaatatta acacaaaagg gtgatggtat
    21121 cataatagaa aagcctgtat actatccttt tgaagataaa ataactaata atgaaagaaa
    21181 agtagttaat agtcctttaa agtttgaaga tggtcattat tctatggatt atgatggatt
    21241 tgaagaatta gctaaagatg aaaataataa ggtgtttata ttatgtagtc ctcataatcc
    21301 agttggtaga gtatggaaaa aagaagagct tagaaaaata gtagacatat gtaagaaata
    21361 tgatttgtgg ataatatctg atgaaataca ttgtgatatt attcgtaaag gtgaaaagca
    21421 tataccatta gaagtagcat gtcctgatta taaagataga ataataactt gtacagctcc
    21481 aagtaagagt tttaacttag ctggtatgca attatcaaat atagtaatca ataataagga
    21541 attacaaaaa aaatggatgc ttgaaacaga ttctaaatta gctatattcc tacctaatcc
    21601 attcgcaata gttgctacta aagttgctta taatgattgt gaagattgga tgaatcaagt
    21661 aaatgagtat atagatggta atattaaata tgtagaagat tttgttaatg agtatatgcc
    21721 taaagtaaag ataataaaat ctcaaggaac ttacttagtt tggttagact ttagagggta
    21781 tggattagat gcaaaacagc ttgaatatgt aatgcttaac aaggcgaagg tattgctaga
    21841 tgaaggatac atattcggag aagaaggagc aggatttgaa agaataaatg ttgcttcacc
    21901 tagaacaatt atagaagagt gtatgaatag aataaaatgt gcatttgaag gattataata
    21961 gtaatatcta aataatatat ttgtgtattt taaaaaaaga ctatgctaaa aaaatatttc
    22021 aaagcatagt cttttaaagt tttatgatga gtttaaaaaa tttcaaaacg ttactattca
    22081 cttatatgtt tatattagaa ataattccac aatatactca ttttctaaag ttgcactttt
    22141 atacaccttg cttcaccaat atcatgtgta accaaaatag tagacttatt ctcattctta
    22201 ataatactat aaacatcatc acaaactaat aatctagtct gataatcaag agctgaaaaa
    22261 ggttcatcta aaagtagaat atctggattt acagaaagtg ttctaataag cgcaactctt
    22321 tgtctcatac ctccagataa ttcttttgga tacatacttc taaaatccca taatccataa
    22381 gtctttaaaa ggctttcaac tctatctaaa gattttttat cttttttgcc ttgaatttcc
    22441 aatccaatag ttatattatc cattatattt ctccattcaa gcaaattatc tttttgaaac
    22501 atatatccaa tatttccagt aatctttata tcaccactag tcggttttaa aagattagta
    22561 agaatattta atattgtaga tttaccactt ccagaaggac caagcaaagt taatatttct
    22621 ccttctttca agttaaaatt gacatccttt aatacttcaa gctcgccttc cttagtgtag
    22681 aagttcatat ttatattttt aacttctact atgttgtcat tcattatgcc accccctata
    22741 acttttagct cgtatatatt atgttattaa caatatgacc tatttgtacc atttttcata
    22801 aatataatat aaatattttg tttatttacg tattttctta gtaacttgta cgtataaatt
    22861 atagataaat aaattataag gtaggggata aatttgagta acataataac gaatttaata
    22921 gagtcaatat ttactaatcc aataactata ttgtttttac ttgcaataac tgtgtattct
    22981 gtttttaaaa taatagaaaa taacagagta tattcatttg taagttcaaa attagacgaa
    23041 atcaatagag actataagac aagtgagttt tataaaaagg taagggatga ctactataca
    23101 tatagtaaag aaaatcctta tgcagacgta aatataactt catttataga ggaagttgta
    23161 tctgatttaa agcataataa tttgccattg ttagaaaaaa ttagaagtat aaaaaattca
    23221 tcttcaataa gtatattact tggggtttta ggtacgtttg ttggtctttc tacaatgctt
    23281 ttatgtgttg atactaaaga cattataaat tcacttcctt caactattag tagtatgcaa
    23341 actgcattta caactagtat atttggagtt gtattttctc taattatagg ttactttact
    23401 aagataaaag attgtgaaca tgttttgata caaataatgt taaaatctga aaatttacta
    23461 acatctgaaa taactcattt taagtcagaa agaatggatt taaaagtaga agaggtaaaa
    23521 aatacaataa aacaaataag taagtctata gaagctattg aaaaatttga taaaatatct
    23581 aaagatttaa atgactttaa tgatgaattt atcagtggta tagaagcttt aaaaagcctt
    23641 ttagaaggtt ctcaaagttc tattaagaca tttgaccaga gcgtaagaaa attagataaa
    23701 cagtttaata ttttaaatat aaaatttgta aagctttttg ataaatatga taatcaagat
    23761 aatataaata aagagatttt atttgatata aaagaatctt caaaaaatat atacaatgcc
    23821 actgaaagcc aattcaaaat tagagattat ataaaaaata taaatgcagg atttgctcta
    23881 tatgaaagaa gtgctcaaga tttattaact aaattaatga cccatgaaaa taaaatatct
    23941 caaaatcaaa aaatcctttt agatgaaaag tttaccttag atgactcaat aaaaaatctc
    24001 tcaagtatta ttgaaaactt ttctaatgac ctacaagtta aactggatat gatgtttgaa
    24061 aattctttag atattcaaga taagttagat gttatgttta ataactcttt tatgaatgat
    24121 gaagttcctc ttgatagtga agaattattt aaggatgatg ttaataatgt atttaatcct
    24181 tttagtgaag aaatttatga gatagaggat aaagaaataa aggtcatagg tgaggatgag
    24241 ttaaatgaat aataaatata gaagaactgt taacagagaa tttgaaaaga gtagcttttg
    24301 gccaacattt acagatttat tatctactgt attgatggtt gtgattctta tcttatttag
    24361 ctcagaaagt atttcaggtt ctgttgaaca agatttagct aagaatgtca atgcttctgt
    24421 tgaagaaact tttaaaaaaa gtggaatacc tgtgaaggta gataaatcta atgggcaagt
    24481 aacttttggt gaaaaaacca tgtttgatgt agatagtgat gttttaaaac cagaggctaa
    24541 agaaatgcta aaaatgtttg ttccaaagta catagagact atttataaag attatggtga
    24601 ctatatatcc aaaatagtta tagaaggtca tacagatgat gtgggaagtt atatatacaa
    24661 cttagattta tcgcaaagaa gagcatatag cgttgctaaa tttatagtgg gagatgaaat
    24721 aggtgactat aaatacaaag ataaagtgac taagcatatt attgcaatag gaaggtcaaa
    24781 agctgaactc atcaaaaatg gtgacaatag cgtgaataga gatgcatcta gaagagtaga
    24841 actaaaatac gaaataaata ttaatcaaaa taaataatta aatttttata aaaaaaaaga
    24901 aggcagttgg tgtgagcttt aagccttctt taaggaaaaa gtttatttat ttttgttgat
    24961 acttaacata ttatagtttt tagaagcagt ttgcaatata ttaaacgtaa atggtcgttt
    25021 aaaatgcata actggatact actgatgtaa aatatttcat tttatataat ataaaaatta
    25081 tacagtgtca attttctata ttattgtact ataatctatt atttaaaata ttatgtaaaa
    25141 aaatatcata aaaacgcata tcatgttata attactagag tattatgaat atggaggcgt
    25201 agtttgtgat agaaataagt ataaaaaata aaaaaaattc taaaattaaa actttgatac
    25261 ttttattgat agtatctata tcgatttatt taactggttg tactacatca gataatggaa
    25321 ataatcaatc aaataataat gattctttgt ctagtagtat atcaagtgat aagaatgtaa
    25381 aaattcattt tattgacact ggaaatagtg atgcaatatt gattcaagat ggtaaaactt
    25441 ttacattgat agatggggga gataatgatg atgagggatt aatggtagat tacctaaaca
    25501 atcaaggtgt aaaagatatt aaatatctta tagctaccca ttctcatgca gaccatttag
    25561 gtggacttga ttctgttgtc aaaaacttta atatagaaaa tgtttttgta tcgaatggtt
    25621 cagcagaaac aaagagttat agggacttta taaatgcttt agcaaataag ggtttatccc
    25681 caagtgtacc gcttgaaaat aataagttct atttagaaga ttcttatttt gaagttctaa
    25741 atacaaatgg gggagatact actaatgaac aatctctagt ccttgtatat actaatggga
    25801 atgataaagt tttatttaca ggtgatgctg aagaaggtac cgaaaaagaa atactaccta
    25861 agcttgagaa agtagacctt ttaaaagtag ctcatcatgg ttctcgtagt agttcatctc
    25921 aagagttttt agataaagta aatcctgaat atgcggttct tttagtaggc aagggtaatt
    25981 catatggtca tccacatcag gagacaatga ataaattaaa aaaaatggga gtaaaagtgc
    26041 atagaagtga tgagtgttca gatataatat ttgaatcaac aggtgatgga gtatttacaa
    26101 gttgtaaaga tggaagttat aataaaggcg ttagagaaga tggaaactct ggaaaatcaa
    26161 acagtaatac aagtaaaaat gattctttta aaaataaaca aaatacatcc aattcagaag
    26221 aaatagtgta ttttactcca aaaggtaaga gctatcactc tacaaaaaat tgtagtgggc
    26281 tttcaagaag taaaaaaata ttatcaggca caattgcaga aagtaagaaa aatgacccat
    26341 gtgatatatg ttatggaaaa taacaaaaaa acaaccaatt tatctataaa gcttctgaac
    26401 tttaaatcat ttaaaaaata aaataaatgc ataaaaaaat aagctataat caatagataa
    26461 cacattttat ctatacgaat agcttatttt tttaagtatt ttttaatagc tatttaataa
    26521 ctctaaatac accagttaca atacctacaa tttcaacttc tgagtcattg agcataattg
    26581 gttccataaa atcattttct ggctgaagtc ttattaaatt tccttcttta aaaaacctct
    26641 taaccgtagc agattcacca tttataagtg caactactat ctgagaattt aaggctgtat
    26701 tttttttgtc tactacaacg taatcttcat ctaaaattcc tgcattaatc atactttcgc
    26761 cttttactct taatacaaaa ttgttactgc cctttactaa actggcaggg aatggaatgt
    26821 attcctctat gttttgttga gctaaaatgg gttctccagc tgttatttga cctactaaag
    26881 gaagctctat tatttcttga ttagctccag aaacatcatt tactttacta cgttctaaaa
    26941 cttcaatagc tcttggttta gtagggtcct ttcttatgta tcccagtttt tcaagtttat
    27001 ttagatgaga gtgcacagtt gaagtagatc ttaaccctac agcagtacat atttctctta
    27061 cagcaggtgg ataacctttt aatatgattt gagactttat aaattccaat atcaagactt
    27121 gcttttcagt tagatctaaa tacataacat cactcatttc caaacatatt ttgtttttat
    27181 ttccatacac tttatatttt taattataac ataatccatt tatatctgca aacatttgtt
    27241 cctgattttt aatcgtcctc aatccattca aaattcttga ctatatcacg ttttacattt
    27301 ttcttatgag ctgcataaag ctgagtagtt gtgacattat catggtcaag taagttttga
    27361 acatcatata tagaaaatcc tgtttggatt agagaagttg cagccgttgc tcttaattta
    27421 tggggactat atccattgtc tctagtagta tcaagtggaa tagatgtata tttttttacc
    27481 agtgttctta tagcctttgc tgtaattctc ttattttgaa gagacaaaaa taaagcatct
    27541 ttttcttcat catttaatag ttcatctctt gttctttcgt tttgcaaata atcttttatt
    27601 acatgttcac aagtatgatt tataggcatt aaaacttctt tcccacgttt tctatatatt
    27661 ttaaattcac ctcttgaaaa attaaaagag gagatattga gttctctaag ctcatttaat
    27721 ctaagcccgt atgtaacaaa taatgctagt atagctttgt cacgtaattt agtttttctc
    27781 caatatacct tttctttctc tgtaagccct tcacctgttt ctactgattc aagcatctta
    27841 gcaacttcat ctatctcaag gcgcttaata gcatctggct gaggtttagg aagttttatt
    27901 ggattgaatc catcagttat attattatct atctgagagt ttctgtataa gaatttaaat
    27961 agagtagaga tggatgattt ttttcttgct aaagctctat tgttattttc aaaaatcaat
    28021 gtgttttttt ctgtattttt gtaatatcta ctgcaataat cacctaagaa taaatttata
    28081 tctcttgatt ttattgtatt aaaatcatct tctgtaatgt cttttacaca atctgcattt
    28141 gttaattctt tggtttcaat caaatatgaa caaaaaaaat taatatcttc taaataagca
    28201 agtcgtgtag aaactgcaac agaacctttt aaatatataa aataatcctt cataaataca
    28261 ggaagtttat tttccaaaag tagacatttt tctatgagtt cattttcttt gataacaata
    28321 ttatcaggtt tatctgattt attatgtact tttataattt tcttatcatt attcaaaatt
    28381 aaacctccta aaactaaaat ataaatcttt tatattattt tttgatattt atactaagtg
    28441 agtataaata tttaatttaa tcatttttta catttttaag agattagcag tgctttagat
    28501 gaaattatca ctaactattc cctaaatcca catttaaaga ctagttgaat ttaagtactc
    28561 ttttttattt ctcaatatat ttatataaat atattgagaa attttttatg ctctactaga
    28621 atctttttta tataatattt ttatatgttt ctaacgaaac caaatattag taaaatatgt
    28681 atttatttaa ctaaagtttt taaatgggct gactaagctt aaaatatagg atattatgag
    28741 agttactatt gaaaaaataa accctattgc cataaagaaa cttataaata tgttatactt
    28801 aactattaaa ctggatatat tcaagttatc aaatccaaat aagtatgtaa ttgaacttat
    28861 tacaaaaata actataacta taaagctcag gccatttaag ctatttttta aatcagcact
    28921 actaagtgac atatgtgtag atatagaata cattaaaaac ataaatataa aatacttcaa
    28981 tgaatataca ctaggtgtta acaacattat tcttataaaa ttggaaatat cacctaaaat
    29041 aatgtgtata aaaccattta tatttaaatt attaatcatg cttacatata actttaaatc
    29101 tatattttga gtcactttca tataactatc tggcagtaat atcttaaaaa ttaaaataat
    29161 cattattgtt ccacctataa taggtgcaat tcctataaag aaatttccta tactttgata
    29221 aagattatct tttttatagc tatgacttac atatcctagt ataccatcat ttttactttc
    29281 tattggtctg aaaaacttaa ctgaatttat tttatgcatg aaaattaaac acattatcaa
    29341 atgactcaat tcatgaacaa atgttcctat agctccagtt acaataattc cagtttttcc
    29401 aaaagatgaa aatataaatt tattatttag attttctgta atgctaaaaa tcaatccaaa
    29461 tacaataaat attcctatga aacaaaaaat atttattaca cttattttta aaatttcgat
    29521 tatataaatc ataagttaaa tcctcatgta aaagtattta actactctac ttcatatcca
    29581 ttacagctta aaattttaca agcattttct atatcctttt cttttactaa gatatagtct
    29641 gtatcataag tagaaattgc aaatatgctt attttgttgt ctgctaacaa actacttaac
    29701 ttagataaaa ttcctacaag tgaaaagtct aaaggaccta gtatcttaag tattctccac
    29761 tccttttctg acttaacatc agaaggaata ttattattta aacatacaac acttaattca
    29821 tcatcagttc tagttataga gtaaaacttc tctgtatcta tccatgttgg tattttactg
    29881 tcattattta atctacatac tgcatactct tctaccaata attttaattt cataatgtat
    29941 atttcctcca atcttatttg tttacttaag attatacatt aaaagttaca taaaataaaa
    30001 catataaagt aaaaaaggtt cactatagtg tcctatttct atagccaacc tttaaaactt
    30061 aattaaattc taataaagag atatattatc taggttcaat tataagttta atggctgttt
    30121 tcttatcacc ttcgatttct atatcagtaa atgcgggaat gcaaactaaa tccattccac
    30181 taggtgcaac aaaacctctt gctattgcaa ttgattttat cgcttgattt agagctccag
    30241 ctcctatagc ttgtatttct gcaacgcctc gttctcttag tactccagcc aaggctccag
    30301 ctacagaatt aggattagat tttgatgaaa cttttagtac ctccatttca acatctcctt
    30361 gttaatttgt taaagtatcc attacctaaa gtactaaatc ataattgcca tttactaatt
    30421 acaatcatca tttaaagtat acttttataa aaatatactt ctataatcgt tttaagaaaa
    30481 cctttatttt ttttaaccat tttattatga aaaaactaca aatttcttta tattctataa
    30541 gcattttcca atatttctaa attttttata tctttgagct ataatttcct ctttatccat
    30601 atctttgtaa tgatttaatc tttctaaaat gtaagctttt atttcttttg aaacagcttc
    30661 tacatctttt tgtgctcctc caagaggttc ttttataatc ttatctatta tttttaaatc
    30721 atataaatca ttacttgtaa gcttcataac atcacaagct tctttagctt tagatgaatc
    30781 tttaaataga atactagaaa gcccttctgg agaaatcaca gaataaactg aatgttctaa
    30841 catacataca tcatttccta ctccgatacc taaagctcct ccactaccac cttcaccaat
    30901 tacaaaagtg attactggaa cagaaagttt actcatttca agtaagtttc ttgcaatagc
    30961 ttctccttgt cctctttctt ctgcttctag accacaaaaa gctcctgatg tatcaataaa
    31021 cgttattata ggtctattaa atttttcagc ttgtttcata agtcttaatg cttttctgta
    31081 tccttctgga tgaggcattc caaaatttct ttttatattt tcttttgtat ctcttccttt
    31141 ttggtgtcca actatagtaa cattaaactt tcctatctga ccaattcctc caaccataga
    31201 agggtcatct ttaaaaagcc tatctccatg aagttctaaa aaattagaac aaatatgttc
    31261 tatgtattcc aatgtcgtag gtctctttat atctctactt aatgttacct tttcatatgc
    31321 tgatagatgt gaaaaagcat cttccttaag cttttccaat ttctcattaa gtgaatttat
    31381 tttatcactt aaatctatat tatttgcctt agaaatagtt attaattgtt taatatcatt
    31441 ttctaagact tttatttttt cactattatt ttcaatcaaa acagttcctc ctttatgccc
    31501 tatttcttgt gcatagcaag aatttgatat aatacttcct tcatttttct tctatctact
    31561 atcatgtcaa taaaaccttt ttcaagtaaa aactcagaag tttgaaatcc ttcaggaagt
    31621 ttttgattta ttgtttgctc tattactctt ggacctgcaa aacctatcaa cgtatttggc
    31681 tcagaaatta taatatcacc aagcatagca aaactagcag ttactcctcc agtagtaggg
    31741 tcagttagaa ctgatatata tggcaatgat ttttcttcaa gcttagaaag tgcttgagaa
    31801 gtctttgcca tctgcataag agataccata ccttcttgca ttctagctcc tcctgaagca
    31861 gaacaaataa ttataggtaa attattttca gcagcatact ctattgaata agttattttt
    31921 tccccaacaa tagaacccat acttcccatc ataaaatcag gattcattac acaaataact
    31981 gttttaattc catttattct tccataacct gtgacaacag cttctgactc ttttgttttt
    32041 tccttataag agtctacttt ttctttgtat ttaggaaaat ttagtggatt ttgaaattct
    32101 acatcagaat taaactccat aaaagttcct ttatcaatca ataaatttat tctttttctt
    32161 gctcttaagc cataatgtct tgaacatttt ggacatacaa atgaattttc ttctatgtcc
    32221 tttctaaata catgactatc acaacctttg caataggtcc aaaatttatc atctacacta
    32281 ttctttttaa agttgtcatc taaagaaatc gtaacatact tagattcttt tgatgataaa
    32341 aattttttta tcataatcta tttactcctt ataatgtagc aaattctttc ttcataaaga
    32401 catcttctat aaatttggta tgatgagtat cttctctaaa ctcctcactt gttacaagtg
    32461 ccttttgaaa ttctacattt gtattaatac cttctacaat aatttcgtct agagctctat
    32521 atattctact tatacattct tctctagttt cagcccaaca tattaactta cctatcatag
    32581 aatcatataa aggtggtata gtatatcctg catatacaaa agtatcaaat ctaacaccaa
    32641 aacctccagg taaatttaat gattctatct tacctggtga tggagcaaaa ttctttgatg
    32701 aatcttctgc atttattcta cattcaatag catgacctct aaaagtaata tcttcttgtt
    32761 taaaaggaat tttatcccca ttagctatag atatttgaag ttttataagg tcaagatttg
    32821 ttaccatctc tgtaactgga tgttcaactt gtattcttgt attcatctct ataaaataaa
    32881 agtttttatc tttgtcaact ataaactcaa tagtacctgc accgatataa cctactcctt
    32941 ttactatatc tactgcaatt ttatgaagtt tttgtctttc ttcatctgaa aggtatggac
    33001 ttaaagattc ttctattact ttttgatttc gcctttgcat agaacaatct ctatcaccta
    33061 aatgtattgc atttgaatgt ttatctccaa atacttgtac ttctatatgt ctaggatttt
    33121 ctataaactt ttccatatat aaatcactat tagaaaatgc tgctagagct tctgaacttg
    33181 ccatatcaaa atttgaaaaa agttcttctt cttttctaac gattctcata ccttttccac
    33241 cgcctccact tgaggctttt atcataacag gatatccaat ttctcttgca acttctaaag
    33301 cttcttcctt agttttcaaa acagattctg aaccaggaac aacagggata ttcaattcca
    33361 tcatagtttc tcttgctcta gatttatctc ccatcaaaat catttgttct cttgttggac
    33421 ctataaaaat aatattatta tcttcacatt gctttgcaaa ttcaggattc tctgataaaa
    33481 atccaaagcc tggatgaata gcatcacact ttaatttaag agcaagatag attatatttt
    33541 ctatattatt ataactctta cttatattat ttggtcctat acaaatagat tcatcagcta
    33601 tatatctatg aaaacaatct ttatctattt cagaatagat agcaacagtt tttataccta
    33661 gttctttaca tgttctgatg attctaactg ctatatcgcc tctatttgca actagtattt
    33721 tttttatcaa cttaaatacc tcctactaca atggttttat cttaaataaa ggttgattat
    33781 attctactag ttcttcatta ctaactaata cttcaattat ttcaccttta atttcacttg
    33841 ttatctcatt cataagcttc attgcttcta atatacataa agtgtcgcct tcttcaacta
    33901 catcgccaac tttaacaaag ctatctgcat ctggacttgg agaatcatag aatgttccca
    33961 tcaaaggagc tactactact gatagattct cattagatgt gacaggtgta tcgtttacaa
    34021 ctggcttttc taaagaaaga tccactactg attcttgttg tacacttaaa acaggtgaac
    34081 ttgtagattg agctttttta gatacttcta atcttaaatc gctactttca agttttacat
    34141 attctaagtt agttgagtct atggccttta ataattcctt aatttcattg atgttcattt
    34201 tgttaccccc ttgtattatt acctggtgct aatattatat cacaaaaaac aaatttataa
    34261 aagaaaaagt gctgactaga gaatcaacac atcaatatat cttttaattt taaattaaca
    34321 accagtaact tatatttgtt ttaattttcc attattaata taaattataa gtataaaatg
    34381 aataaaattc aatatttttt gcaaaataca agagaataca aattaatacc tcgggaattt
    34441 ttaagacaaa taccaatatt atgcaattta gacttcgctt tctttcaaat tagtatcatt
    34501 ttctttattt ttacatagga tgtactttaa tttagctatc aaaacattgc tatataggtt
    34561 aaaagttatt agaattgcag gaattataaa gtaaactcta gcacctataa aaccaaaaac
    34621 tactgtaata agtattaatc ttttatttaa agtaaaaaaa ggtatattaa aaatactttt
    34681 gtcggatgtt tctggagtat aagaatatat aactccaaaa atcaaagtaa gcatacaaag
    34741 tggaagtagt gtataacttg aaattattcc caaaaatcct atactttcaa aatatttgct
    34801 agcactattt gtataaacaa acatgtatcc atttaaatat gcgcatatac atgatataat
    34861 gccaaaagtt ccccctaagg attgagtgta ccaattaaaa aatttagatt ccatatacct
    34921 cacctcctaa aactgtatat ataatgatat gcatttaaat agtaaaatat atctttatat
    34981 gtttctgaat aaaattttgt taatttcatt aatttatgtt taagaaattt gaagtatagt
    35041 gtagtatata ttaatgtaga tgaagtttta tagtacttat ttcatagtgc ttaatcaagt
    35101 tatcttatta tggattcaaa agttgtttgt agggttttat attttgttga gctgaaaaat
    35161 ttattataaa tgaagggatg ttttcgcttt ggtagataca ttagaatttg gtcttaaaat
    35221 tttatttttt atattatcaa taatttggat gggtaaaata atgattttaa gaactgataa
    35281 gcaaatagta atcaatcctt tgcttattgg tatatctgca atccttgtga tgttgcatac
    35341 ctcacagagt aacattgaat tttttggatt agatgtccaa tatataagaa tagttttgta
    35401 tataatatat tcactgataa ttttaatagg aatatgggct actaacagaa gaaatggaat
    35461 tttttagata atctatttct ataaaattta aaggttgcta gtaacaaatc actaatatta
    35521 gcgttctgtt actaacaacc tttattttga ttatacactt aaattgaaaa tagaatgatt
    35581 gaataagttg acagattatt ttacattgac agtaattaga acttcttgag atgtatttaa
    35641 atcatttttg tcgcttagta taacttttac cactgcttta cctgttttat tacctttaat
    35701 tatcatatct tcacatgaaa gtacactctt atcacttaca gatacaataa aatttaaatc
    35761 ttttggaagg ttatcagtaa cttcccagta tttataataa ctatcaattt tcttcgtaat
    35821 tatttttctc aaatcaatac tttcaccttt tgctatatca acattatgat atttagagtc
    35881 cagtttatac tgattatctt ttgtatcaat aaaatttaaa tcgcaattta catccacacc
    35941 attatgagaa agagaattta ctacatcgtc atcaatcaaa taattattgg caagatttat
    36001 tgaatccata tcttttagac ttaaagcttc cttaggaacc tcttttatat tattattcca
    36061 aaaaataaaa ttgtctacat tttcaagata tgatatacct tttaaatttg aaatactctc
    36121 accagttgca taaaattctc catctaaagc ctttatattg cttatagtca tctcatctgg
    36181 atttatatat ctttttacaa ctgctcttaa atttgcatca ggaaaaatat ctttaagttg
    36241 attttcgctc aaaaccttat catttgaatt tattggactt aaagctgctg cagaaataat
    36301 aaatggtatt aaattagcta aattcatagt accctcccac ataaattttt tattataata
    36361 atttaatttc tatgagaggg ttctgtttcc ttgttatata tacctacttt cgtacacttt
    36421 attaatataa attaaaataa atttctacta ttaaacttat aaacatatta ctagttacgt
    36481 tttttagctt cacatctaaa tattcgcata ccttgtgaca taaatttgtc ttcatactct
    36541 gttgtaacat tattttcata ctcactattt cctaaatcaa gagaaatatt ctttaatagc
    36601 caattatttg cagcaatttc atttaaacta aactcgaata atttttcatt atctgtttta
    36661 aagtgaattt cgccatcatc ttccaaaacc ctattgtaca tttctaaaaa tccagaatga
    36721 gtgagtcttc ttttactcca tctattttta ggccatgggt cacagaaatt tatataaatt
    36781 ctcgataatt ctttagcttc aaagtaatcc aaaatattgc ttacatctcc ccataaaaat
    36841 agtatattat tcaaattaga agcatctgct ttttcaacag cttttaaaag aacttcttcc
    36901 ttaagttcca tggcaatata atttatgtca gggttctgct tagctaaagt tgttataaac
    36961 ttaccttttc cagtcccaaa ttcaacatgt attggattat catttctaaa ctttaaattc
    37021 cacttgcctt ttaatttgtc tatatcgtct cttaaaacat attttgtata acttaaaagc
    37081 ttttcatctg cacctttttt tcgtcttctt ctcacaataa ttctccttac atataaaaaa
    37141 ttagtaaaat acatttttat tatacattat ctcaccaaaa aataaaaaga actctcttaa
    37201 aatagaaatt tatttctata aagagagttc taaacttact aaaaacttaa tcttagtggc
    37261 accctgaaca accagagcat cctcctccac cttgatcttt catattttca ggaataactc
    37321 taaatccaaa tcctgtatct tctattatta tatctccata ctgactatat tcatcagagc
    37381 tcattacaaa ttgtaattct ccaacttcat aagttaagtc atcttctttt ttctcatcta
    37441 aagctaatcc aaaagaagga cctccacaac caacacctgc aaagtatact cttatattat
    37501 taggtttatc ttgattgtct tttaagatac tttttaaagt atcaatagct gtttctggta
    37561 aagtaatttt catcaaatca ttccctttca aatattttat taaaacaata tctattatat
    37621 acccatacaa ggtacataat caattagttt ttgcatattt tactaaaact tctacctaat
    37681 attacctaag gctttaaaat attttatata atattttatt accttaaata aataccctat
    37741 cataaactta gtaaacaaat aaaattatta ttctaaaaaa tataataatt ttatttgttg
    37801 ttttaatatt ataacacatt tgataatgtc caattcatat atgcaaaatt tatatacttt
    37861 aaaatatcta agaaattatt caataaaaaa atactatttt ttaaaaatta tcattattga
    37921 taaagctcta ctatttatat tttttagtgt attcatgtat aataagtaaa agcacaacgg
    37981 tatatactgt tgtgcttttt tgtaattaat aaacaatatt ttggtctata acaatgaaaa
    38041 atggctactt tccataattg atttcatctg acataagcaa aaagggtgtc cagctgggtc
    38101 aatcataact ctccagtcat cagtaaattg ctcctctgcg attgtagctc cacaatgaat
    38161 tgcatattga actgcttctt ctaaatcctt aacagcgaaa tccatatgag ccatttgctg
    38221 ttgagattca ggtttttctg gttctattat gccccaagcc tttgtttttt gcaatataaa
    38281 gaaaaaatta tatattttaa tattttatac ttgttgaaat tgtgtaataa attcaactgg
    38341 atacatagtg cgttttgaaa tttcttctaa agctaaacct tgttttaaaa gcctctttat
    38401 gacgctttcc atactttctg aaatttctat aatattttta tcaaaatcat aaaacctaaa
    38461 agttctttga ccccatgggt attcttttat ataatgcaaa aattcaatat ttcctattct
    38521 atttatctta ctttcccatt gtttgatgtc attaacctca aaatacaact gaaagttatt
    38581 tgattgttga tatattggta aatcatatcc aattatagct ttataattac tttgtaatcc
    38641 aaatccattt tcaaatgata catgttctcc taaatctaat gtgatttttt gctccataac
    38701 atcttcaaaa aacttttttg atttatttat gtctttgaca attagcaata ctcctccaaa
    38761 tttcatctta tcactatcct ttctttgtta taagtattat aacttaaaat tttgcaattt
    38821 cactgtaaaa atcagacata ttagctcggt atttttgagg agttattcca cagacaattt
    38881 taaaatcttt aataaaatgt ggtatatcat agaatcctaa ttcattacat atagacatcc
    38941 atgaagtttt attattgttt agaagtttta tagatttatt aatacgtacc aaacgagaaa
    39001 atgacttcat actcaaccct agatactgat taaagagtct gtttaaatgc ctagaactat
    39061 aaaatacttt tttagaaatt tcttgtgaag atatatttcc agagttttga ataatcaact
    39121 gaattgccaa aataaactca tatgagtatt caattttaat attagaaatc aataattctt
    39181 ccaactttaa aatcaattta tctatagata aagattcatt aaatattttt ttaatttcta
    39241 aatctaatga agtattaatt atagaaaatg gaacaacttt atcaataagt tcactttgct
    39301 gtatacctat aagcgggaaa aaaccagcag gttgaaattc tataattagt ataacatcac
    39361 atctattagc aatatttcct actgtttttg gtttagtagt tggaccaaat aatagactat
    39421 gtaaccctgt ggcatcataa aaaaacacca aagttacact tccatgagga ataatcgtgt
    39481 aattatcaga aattattgtt tgatttggaa acgatatagt aaaattcgat atccaatttc
    39541 ttaattgtat acaaggcaag agataaacaa aattgtcttc acaaactata agtgacctat
    39601 ttttatttat aaaatgaata ctttcttttt ttaacatata ttatatcctg tcctatcgtg
    39661 aatatttatt aaagtaagaa gaatccatat tatctctata tatcattaac tgcttttaat
    39721 aatagagata gtatgaatac tcattagata tttaataaac aaataaaatt aaaataatca
    39781 ataaaaaata tagtactttt atctgttatt ttaatattat agcacattta tagtatttaa
    39841 cttatatcca ctttacatat gtctaattta tataattaaa aatgcttatc tcaaatacaa
    39901 taattagtcc ttatacaatt aaaccatctg aaactgtaat agtacgacta ctacttttgg
    39961 ctgcttctgg tgagtgagtt accataatta tagtttgtcc attgtctctg tttattttat
    40021 tcagtaaatc tattatttct atccctgttt tactatctaa atttccagta ggctcatcag
    40081 caaatagaat ctctggtttt ccaattaaag ctcgagctat agcaacacgt tgttgctgac
    40141 caccagataa ctctcgtggt gtatgtttac gtctatcagt taaaccaact atatctaaga
    40201 tttcatctaa ttgattttta taatctttta aatttttacc atctaataac aaaggaagca
    40261 ttatattttc ctccacattt aaatttggta ttaagttata aaattgaaat acgaatccta
    40321 tatttctacg acgtattatg ctcattttct catcattaaa atgtgatata tcagctccat
    40381 tcatatacac ttttccgctc gttggagtat ctaaaccacc taaaatatat aaaagagtac
    40441 ttttaccaga accagattga cccatgatag aaacaaattc acctttcatt acttgtaaag
    40501 atacctcttt taacactcga gttgttgtat ttccaatttt atattctttg ataatatttt
    40561 ttgcttctac agctaaacta ttatgtgtaa ttttcatatt aacctccata attattcaaa
    40621 cttaatttct tctatcagtt tcattttctt cccttttata ataggcacta tcgaacctat
    40681 taaagttact ataattccca ataacccaac aataataaat gttttaaaat ctaaatctgg
    40741 tttcattgaa atttttggtc ctgcaactat aaagatagtt tgaatctcaa gtatagatat
    40801 cacaatacca ataacagctc caagtaaacc tgaagtaacc ccttcaatca atgttacttt
    40861 tatattttgt ttattactta gtcctataga tttatacata gctatagaac gtcttttttg
    40921 aatataatta atcagtagat tattaataat acctactgta gcaagaagaa aaatgaaata
    40981 ggtcatctta ttcataggag acaaaaaact acttatagta tttaaagaat cattattaaa
    41041 ttcttcaact gtacgactcc aattatatgt atctccaaat aaatatctta tctgaatcat
    41101 aacagcatct ggattgactg ctgtatatac taaaaaacca taattagttt tatcaaaatc
    41161 attaactgca taatgtgatg gtataacagc ttctacatca tttgcacggg atttaaaact
    41221 acctactatt ttataagaaa aatcatactt atcattagat agagttattg tatctcctat
    41281 agataagcct acttttttta gagtatcttc attcaaaata acagaacgtt tatcattaaa
    41341 agcttctatt acttgttttt ttatctcaaa atttgtataa ttgattccaa acatagagtt
    41401 atatagtttt atattatctg taccctctag tcttgataat gttacaccat taccagaaat
    41461 ctcattattc ataacataaa gaggtaatat ttttttaatt ccttccatat gtctaacatc
    41521 ttcaataaac tcttcattca tatttccatc tgcaaaacct tgtaattctg cgtcacgaaa
    41581 cacatctgtt atataagttt tgacaaagtt accaacaaca cttattgcaa taactgcaga
    41641 aatacttata aatagtaaag tgatattctg tattatattt ttgttatttt tcatatttct
    41701 agcagctaat ttaccctcat tccctaaaat atttttataa acaaactcaa aaactataga
    41761 catgatatca gtaataagag gaatcaacac aatagtagct acaattaatc ctagtaatga
    41821 aaatccacca gctagataca atgtattttc aggggatatt cttggaagta aaatagataa
    41881 aataaacatt atacttccta taaaaagtat agaacgattt gaaacattct tttcttctac
    41941 tgttcctagt acaatatttt ttatcggcaa atggctagct tttttaacag gtatatatgc
    42001 actaaatgaa gaaacgatta ttgctactat aacagatata atcactccaa tgggtgatat
    42061 tactactgga attgatattc cttgttcgag agaatttcct aacccatgaa gcattaaatt
    42121 taaaacaact acaccaatag gaatagcaat taatcctcct atacttccgt ataatatact
    42181 ttcaagcatc aatatacgag ttacagtttt ttcattggca ccaatacttc taaatgttcc
    42241 aataaaagga agacgttcta atgtaataac cttataactg ctgtatatta taaatatact
    42301 aagggttaat gcaaaaaaac ttattaaaaa gaaaggcatt gtcttttgtc tagcatctga
    42361 aattatctta gtttcattta tagtatttga taccatatat ttttcagtag aaagttcttc
    42421 ggataattta tttactagat tttctgtaat ttcttcttct gactctataa gaactcttgt
    42481 atatccactg cctttattta taatttttga tagagtctct ttgggtagca gtgcatttac
    42541 acctcttgtg ttcctcaaaa atactgtatc ataagatgca atatcactaa cttgaaaagt
    42601 ataagacttt ccataaatct gcaaagtaat agaatcacct ttttttattt tatattttga
    42661 tgtaaagcga tttggaagaa taattttatc accagaaaaa tcagtaatac tatcgccatt
    42721 ttctaatcgt ggcttgttga ttttatttag ttgacttaag tctgctgata taagactaaa
    42781 ttcttcatag tatccccctt tattataaat agcagagctt tccaatacac caacctttga
    42841 ttttatagta tttaaatcag gtatgtcttc taaattggtt aatatatctg gatttttact
    42901 ttggacagat atagtcgctg taccatacat accttttgcc atttttcttt gtgcactttc
    42961 ataagataat ccaattgaga atgaaacaaa aagtaaaact gttgataaaa ctatggacaa
    43021 taacataact gctgttctaa tttttcgttc tttaatattt gtgaaaatat actttaatat
    43081 aattttcaaa cttacacctt cttcccttat ctaagatatt tcaatctaat atttttatac
    43141 tttaatctaa attaaatctc taatagactc ctccatactt ttatcattct atttataata
    43201 tttttaatca aatcacccct taaataaaaa agattgataa actactatat aaatataata
    43261 atctatcaac cttaatatat tttttattta accttaatta accttaatta aataggaaag
    43321 gtgatataaa atgagctacc taatccaggt atactttcaa cagatatatt ccctccatga
    43381 acttcaataa tttgcttcgt tattgctagt cctaaaccag ttccttctat tttttgtttt
    43441 gtattgcttc ctctataata acgattaaac aaatttgatg cttcttctct tgacatccct
    43501 tttccattat cagatataat gatttcaatt ctttcattta atgttacatg tacacaaact
    43561 ttagtatttt tatctccata tgtaaaagca ttgacaataa gattactaaa agctcgtttt
    43621 aatagtgtac aatcaaaatc aaagttaata ttatctatat tactcttaaa ttcaactata
    43681 tttgattcat attctggata atttaaaata tcaatggata attctttcaa gaatcttact
    43741 aaatcataat tatttttatt taaagggatt attccatttt ttaattgata ggttaatttt
    43801 aaatcgttta taagtgtatt tgtatattct atgttcttta acatgattga agcatattta
    43861 tatacttttt caggtgaata attagtacta ctatctgtaa gaagctctgc ataccctttt
    43921 attggtgaaa gtggagtttt taaatcatga gttatatttg aaatccactc ttctctcata
    43981 atatcagttt tattttgtat ctcatcactt ttatgaattt cttcatttaa aatatttaaa
    44041 ctattatata tatcagcaaa aacaccttta atattaactg gaagatagct tcgttttgaa
    44101 atatcactaa cagcagatgt aatatttgat atgcactttg ttacccagta accataagca
    44161 attccagaaa ccaaaattat taaaaatata atccctatga ttgaaaatac aattgactta
    44221 ccacctataa atttacctcc atcaaaaaac atattcactt tagatatatt taatggaaaa
    44281 tatataatat aaatatagtc atttttatta tttttaattg tattaaagta tacagttcca
    44341 ttacttgaat tattttggtt agctaatgct aataattcag aattagaata ataatcttta
    44401 tgctcttttg gttctaagaa cccataaact ctatttccac tagaatctaa aatttgcaac
    44461 cacaattgat tttctcgaag tttttctaat ccagactgtt ttacttgtgg cttattatct
    44521 ataaatacga tttgttcatt aaagttttct gtaaattttt tcggccagtc actacgaata
    44581 aattccccat ttggtttctg aatagtaata agtgataaga aaatacctat tactgtcagg
    44641 attaagcaaa taaaaatcat gaaaaaaata atgtatgaat gaaatactgt tttataacta
    44701 ggtttatgca tttaatcaac tcttttctta aacttttatt ttagtttgat tgttgcttat
    44761 ataaaataga caataattat tgatatgtaa aa
//
LOCUS       DAMJYR010000021        44374 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.021, whole genome shotgun sequence.
ACCESSION   DAMJYR010000021 DAMJYR010000000
VERSION     DAMJYR010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 44374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44374
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.021"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(199..804)
                     /locus_tag="PC471_001103"
     CDS             complement(199..804)
                     /locus_tag="PC471_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HDF2788241.1"
                     /translation="MQNTAKRGNMISTRTLVVMSILSAISYLLMFIQAPIPGIFPDFL
                     KIDLSDIPAIFGGMSLGPLVGFGIVVVKNVLQAITATTTGGIGEFANILIGGSYVMIL
                     CYSYKRSGDLKGVLIGFVLGTISMTIIGSLVNYFVMLPLYGQLMGLDAIIGLGSAINP
                     QVHDLFTFVIWMIAPFNVLKAVIISLVTLPLYKKMGNIIKK"
     regulatory      complement(929..1046)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(1106..2272)
                     /locus_tag="PC471_001104"
     CDS             complement(1106..2272)
                     /locus_tag="PC471_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HDF2788242.1"
                     /translation="MIFIKNGKINTITNGIIHGDILIDEGKIIEIGEDLIAPLDVEVI
                     DASNKLVFPGFIDAHTHLGLWEDGIGFEGADGNEETDPITPQLNPIDGINPMDRTFKE
                     AFEGGITSVCTTPGSANVMGGQCIAIKTCGKRIDKMVIKNPVASKIAFGENPKSCYGQ
                     DDKSPQTRMAIAALLRENLKKAEEYLEDIDMYESHDDEDCEKPEYDIKMESLIPVLRR
                     EIPFKAHAHRADDMFTAIRIAKEFNLKLTLDHCTEGHLIVDELVEEGFPVIVGPSLSE
                     RSKFELRNLTFNTAGILSNAGLDVCIMTDHPVIPVQYLPICAGIAVKHGMKEEKAIES
                     ITINPAKALGIEDRVGSIEVGKDADLVIWDNSPLEIQSNVLYTIINGKVVYEKK"
     gene            complement(2420..4561)
                     /locus_tag="PC471_001105"
     CDS             complement(2420..4561)
                     /locus_tag="PC471_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011010799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HDF2788243.1"
                     /translation="MDINQILKKEFNLRDEQINNTLKLIDEGNTIPFIARYRKEMTGE
                     MSDVTLREFYEKLMYLRNLQSRKDDVVRLIDEQGKLTDEITQNIEKAKTLQEVEDIYA
                     PYKQKKRTRATIAKEKGLENLALSILENNLDNIEIEAKNYLDEEKEVLSIEDALKGAR
                     DIIAELVSDDAKIRKYIRELALREGMIVSKSATDEKSVYDMYYDYSEAVKSMAPHRVL
                     AINRGEKESFLKVKLEINNDKVLNYIINEYVNDKNFKNKEEIVSSIEDSYKRLIFPSI
                     EREIRNHLTEIAQERAISVFGKNVKSLLLQPPVKDKVVMGFDPAFRTGCKIAVVDKNG
                     KLLDYTTVYPTDPQNDVEGAKKVLKGLIEKYDIDIISIGNGTASRESETFVSEMIKEI
                     DSEVQYVIVSEAGASVYSASELANEEHPDINVSIRGAISIARRLQDPLAELVKIDPKS
                     IGVGQYQHDLNKKRLEEVLDGVVEDSVNSVGVDLNTASYSLLEHVAGISKAIAKNIIA
                     YREENGDFTSRAQLKKVKRLGPQAFTQCAGFMRILEGKNPLDNTGVHPESYDICKKMI
                     EIIGYSLDDVKNKNIGEIDEKIKEIGLRELSEKLEVGQVTLKDIIAEIKKPGRDPREE
                     GIKPILRTDVLKIEDIQEGMTLKGTIRNVVDFGAFVDIGIKNDGLVHKSEMSNSFVKD
                     PMSIVTVGDIVDVKVIGIDLNKKRVALSMKK"
     gene            complement(4705..5812)
                     /gene="prfB"
                     /locus_tag="PC471_001106"
     CDS             complement(join(4705..5736,5738..5812))
                     /gene="prfB"
                     /locus_tag="PC471_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_153309863.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HDF2788244.1"
                     /translation="MLKLQEYRHSMEQMASNLEELRVSLDIASLMKNIQINEEKMNQQ
                     DFWNDNEVAQRVLQENKSLKETLEEYESLKSLLEDIEVLIEIGLEEDDDSVERDIEKS
                     IESMEEKLSEMKIKTLLNGEYDKNNAILSINAGTGGLDAQDWAQMLLRMYIRWSESKG
                     YKVKLLDIISDPEAGIKTATILVEGTNAYGYLKSEKGVHRLVRISPFDPSGKRHTSFA
                     SIDVTPELDENIEVEINPSDLKIDTYRASGAGGQHVNTTDSAVRITHIPTGVVVQCQN
                     ERSQHLNKDRAMRLLMAKLIELKELEQKEKIEDIQGKYSQITWGSQIRSYVFQPYKLV
                     KDHRTNAEFGNVDSVMNGNIDLFINEYLKMNKIV"
     gene            complement(5827..8502)
                     /gene="secA"
                     /locus_tag="PC471_001107"
     CDS             complement(5827..8502)
                     /gene="secA"
                     /locus_tag="PC471_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HDF2788245.1"
                     /translation="MSFMDNLFNMADKKELKKFNKTVDIIDSLEPKFESMADSELKNM
                     TNIFKERLANGESIDDILPEAFAVVREVSKRVLGLRHYRVQMIGGIVLHQGRIAEMKT
                     GEGKTLVATAPVYLNALTGKGVHVVTVNDYLAKRDRDQMAKIYEFLGMSVGVIIHGQN
                     PKVRKEQYDCDITYGTNNEYGFDYLKDNMVIHKEQRVQRGLNYAIVDEVDSILIDEAR
                     TPLIISGPGDKSTHLYSDANTFVLTLKPDDYELEEKDKAVSLTASGIQKAEVYFNVDN
                     ITDISHTELYHHINQALRAHVIMKKDVDYVAKDGEIVIVDEFTGRLMFGRRYSEGLHQ
                     AIEAKEGLKIQRESKTLATVTFQNYFRMYKKLSGMTGTAKTEEEEFKAIYKMDVFQVP
                     TNKLMIREDLPDCVYKSEIGKFNAVAQEIIERHKVNQPILVGTVSIEKSELLSQILKK
                     KGIKHEVLNAKHHDKEAEIIAQAGRLGAVTIATNMAGRGTDIVLGGNPDFLTKREMRR
                     NGFKEEIVNRVDTPIEGIPVKGNEILFEAREEYEKLFEKFKQQTQEEQKQVVEAGGLA
                     IIGTERHESRRIDNQLRGRAGRQGDPGSSRFYIGLDDDLMRLFGSDRISGIVDKIGLE
                     EDMPIEHRILSKSIEGAQKKVEGKNFGIRKHVLQYDDVMNKQREIIYAERKRVLEGED
                     LQEQIQSMTHSIIEEAVTLYTQDKGFDEEGFKEHMYNLFLPKGSIEIPEIEKLNPVEI
                     TEKVYEIAMKIYTSKEEQVGYERMREVERVILLQAVDNHWIDHIDAMDQLRQGIGLRA
                     VGQQDPVIAYKMEGFDMFDEMNKHIKEDTVRYLFNITIETPVERKAVVDVENLSSPSD
                     GTLPTSKTVKKDEKVGRNDLCPCGSGKKYKNCCGR"
     gene            complement(8634..9185)
                     /gene="raiA"
                     /locus_tag="PC471_001108"
     CDS             complement(8634..9185)
                     /gene="raiA"
                     /locus_tag="PC471_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HDF2788246.1"
                     /translation="MNIIISGKQIELTDAIKNKIETKLSKLDNYIHPDTDVKVTVSAR
                     KARQKIEVTIATVNGPIIRAEDMQENLYTAIDVVYDKLSKQLKKYKKRLQDKHKDNKS
                     IRFQDAEINLADEQENYGDENQELVIQRRKKFSVKPMSEEEAVLQMELIGHDFYMFKN
                     IDSDEIAVVYKRNNGGYGIIEHE"
     gene            complement(9738..10412)
                     /locus_tag="PC471_001109"
     CDS             complement(9738..10412)
                     /locus_tag="PC471_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HDF2788247.1"
                     /translation="MLEIIGMSVEDAKIIEDCGADRIELVSALTEGGLTPSFGLIESV
                     VNSVKIPVNVMIRHHAKSFVYSKEDISIMQKDISVVKEIGANGVVFGVLDKNNSIDEK
                     NLNVLLKCCDNLDVTFHRAIDESNTIDSVKILKDYDKITNILTSGGKGSIVDNIQMIK
                     NMMLSSNHIKILLGGGLNFNNIEKIKELTKASNFHFGTAIRINNSPFEDIDRQKLKQL
                     VNIISR"
     gene            complement(10415..11467)
                     /locus_tag="PC471_001110"
     CDS             complement(10415..11467)
                     /locus_tag="PC471_001110"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sapep family Mn(2+)-dependent dipeptidase"
                     /protein_id="HDF2788248.1"
                     /translation="MNIISNKIDELKEDLLSDIIDIVKIPSVKGESENGFPFGEKVGE
                     ALNKALEISEKLGFKVRNLDNYIGYAEHGDSDDYVCVIGHVDVVHEGDGWKHQPYKGE
                     ETNGRIYGRGVLDNKGPIMSALYGLYAIKELNLKLDKRVRIIFGTNEESGFEDIPYYL
                     EKEKAPIMGFTPDCKYPVVYGEKGMAKIRIKSKINYEEDVYLGFIENMSENVLVTYKE
                     LNIENSDTILDIKVKYDFSYKLKDVLDEIKASFPNSIDIEVISNFNPVYFDKESNLVK
                     KLQLAYERVTSLDGTPVTTNGGTYAKVMPNIVPFGPSFPGQKGIAHNPDEYMDIEDII
                     LNAKIFANAIYELAKE"
     gene            complement(11516..12475)
                     /locus_tag="PC471_001111"
     CDS             complement(11516..12475)
                     /locus_tag="PC471_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(4)-(beta-N-acetylglucosaminyl)-L-asparaginase"
                     /protein_id="HDF2788249.1"
                     /translation="MWGMIATWRMALEGITEASEHLEKGMEAGDAVELAVRRVEDYPF
                     YKSVGYGGLPNENCEVELDAAFMDGNTLDIGAVAGIRDYSNPVSIARKLSYEKVNNFL
                     VGIGAEDYAHKNGFERKNMLTDRAKLHYKKRKKETLDKGLSPYAGHDTVGMISLDKTG
                     KMCAATSTSGLFMKKRGRIGDSALSGSGFYVDSSIGGATATGLGEDLMKGCISYEIVR
                     LMKEGKHPQEACDIAVNELDKQLIKRRGKAGDLSLVAMNNKGEWGVATNIADFTFSVV
                     TKEESPTVYISNRVDGKTIHKVISNEWMEAYEARIKAPIEVSE"
     gene            complement(12568..13089)
                     /locus_tag="PC471_001112"
     CDS             complement(12568..13089)
                     /locus_tag="PC471_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/betaine/sarcosine/D-proline family
                     reductase selenoprotein B"
                     /protein_id="HDF2788250.1"
                     /translation="MKKIVMILDQIQAGAGGKEKSNIPPAGKSSPLGPGVMMEQFLNE
                     SKVIATLFCGDEFFVNNQEEVTSKMIAMVKKLNPDVVICGPSFNYENFSKMSAILSKN
                     INDKTDIPAFAAMSEENIDVINEYKNDICIVKTPKKGGIGLNDSLNNICKLAKAIANK
                     EDITLMKEEFCYQ"
     gene            complement(13100..14434)
                     /gene="celB"
                     /locus_tag="PC471_001113"
     CDS             complement(13100..14434)
                     /gene="celB"
                     /locus_tag="PC471_001113"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HDF2788251.1"
                     /translation="MNRLERILMPLAEKIGKNKLLIAIRDGFLVSSPLLIIGSLFLLV
                     ANFPIKGWNEFVGQIFGPDWAVKLQQPTVATFEVMTLLAVLGIGYSYAKQIDVDPIAS
                     AAVAIVAFFIITPFVIPYTPEGTESVYMVTGIPLGWMGSKGMFVGMITAITSVKLFAA
                     VVKKGWTLKMPDGVPPTVSKSFAALIPSAIVMVVFFLVKIAFEATPYGSIHEFIFKFL
                     QMPLLKLGNSLGAMAIAYIFLHLFWFFGINGSSVVGAVYNPILKILSAENLAAFQAGS
                     KIPNIITGQFQDMFATFGGAGSTLSLVIAMLLICKSKRIKSMGKLSILPGIFGINEPL
                     IFGLPIMLNPILLIPFAIVPTINIIIAYFCMSAGLVPLTNGVQLPWTTPIIFSGFLTT
                     GWQGAVLQLVLLILGIFMYISFIKMLDKQYLREENETVEEEDDDISFDDLVL"
     gene            complement(14473..14799)
                     /locus_tag="PC471_001114"
     CDS             complement(14473..14799)
                     /locus_tag="PC471_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDF2788252.1"
                     /translation="MKRKVYLFCSFGMSTSLLADKMQKVADEHNLPIEVEAFPIAEID
                     KIVEEKNPDCILLGPQVKYMLKELKPKFEAQGKLIDIINEVDYGTMNGEKVLKLAIKL
                     IKSKQA"
     gene            complement(14823..15131)
                     /locus_tag="PC471_001115"
     CDS             complement(14823..15131)
                     /locus_tag="PC471_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HDF2788253.1"
                     /translation="MEEIVLKIIIHAGNAKSMLYEALDYAKENDFKKADELIENANEE
                     ILKAHKVQTELIQKEAGGDKSDISILLIHSQDHLMTCMSERNLIKEMIMLRKEIQKLK
                     "
     gene            complement(15272..17185)
                     /locus_tag="PC471_001116"
     CDS             complement(15272..17185)
                     /locus_tag="PC471_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HDF2788254.1"
                     /translation="MICLNKRQENLLTTLLLKEEFINVNKIANDFECSERTIRNDCKY
                     IDEWLSTFCNAYIKRKPNIGIKFSGDGTDKILVNQKLRGEVKRVNSELHKQVEILNLL
                     LCNNKKVTLNDLSNKLYINKNIVREEISKLSVALNNYNLNISTKKGSGIYIEGDEKDI
                     RIMLVSFLFKYIDKYRLNIDEIEYFHIVDIIIVKNIMLTIEEDMDIRLTDISFKQLML
                     FLLINIIRIRLGNSLKVSNTKKELKLKMLILDIEDELKSNLSIVLNYEEKLFIESLIL
                     GMNKQINSNNIKKSLYLNEELASYTKKIINLVSEESGINFNNDILLYEQLICHLNVTM
                     HQMKSNVYLENPLLDNIKTKFCFLFTVISSSIEIELKGKQITEDEIGYLTLHFQTSLE
                     RKYSKRESNKKASIVCPFSFGVSMLLKVKIEKRFDNIKIIETLREEDLKRDNFNKTID
                     FIIAFQEYENIQKPIFITTPLFTDEDENKLKEFTNKIKEKDTSYKLMNRLMMSDYLIR
                     ELEHDDIYEAITYLTNLLIEKHYAEKEYLQSIITREKSYPTNVGKGILFPHGDMKYIK
                     QSVLCFARLKTPIKIRNGKDIKIILLLAYKKDDDRKVFRELFKEISNLTEDENMLDIL
                     SKCDIEKVKDILI"
     gene            complement(17517..18299)
                     /locus_tag="PC471_001117"
     CDS             complement(17517..18299)
                     /locus_tag="PC471_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HDF2788255.1"
                     /translation="MKICNDNFIKNFKNMINKCLDFIYPENISCIICDKSIKKTNTYS
                     ICKSCFKEMNFIQDGCMKCGKPIIRHSIEKEFIEECSYCFNKDFYFDKSISCIEYNDV
                     TKKMVLGLKYNQKTFMAKYIAQIMKEKLYLENIKFDYILFVPLHKKRLNKRGFNQAQK
                     IAFNLSKIVNIPLLDCISRKKYTRMLYKLNKKERKEELKNVFVVKENVKLINNKNILL
                     IDDIFTTGLTTNEISKLLKLSGANKVFVLTLLTKANDNYIME"
     gene            complement(18302..20521)
                     /locus_tag="PC471_001118"
     CDS             complement(18302..20521)
                     /locus_tag="PC471_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HDF2788256.1"
                     /translation="MEKLEGMISEIVFKNEDNGYTIAHLVNENDEIVVVGCMPTLAIG
                     ESIEVEGKWVNHKIYGTQFEVNSFMPVTPSSLEGIYVYLSSGMIHGIGEKMAKRIIDK
                     FGVDTLEVIQNSPEKLQEVEGIGSKKVKQIVKSYEEDRELRNIIIQLSPFGITPNYCL
                     KIYKKYKSSAIEVINKNPYQLAEDIRGIGFKVADSIASKIGIDKNSKDRICQGILYTL
                     NKSLSNGHTYLPEHVLIQDSEKLLELNGEIIKECVMMLVYNQKIHIEKVNNENLIYLM
                     PYYLAENGVCSQIVKLSQYEFEDLKIDIDNEINVLEEDKKIKLAEKQILAVKESVNSG
                     VLIITGGPGTGKTTTINAIIDIFENNGKSVTLAAPTGRAAKRMSETSNKEAKTIHRLL
                     EMGFSTDDDLTFFKDEEDPINSDVIIVDEVSMVDIILMYNLLRAIKLGTRVILVGDSD
                     QLPSVGAGNVLKDMINSNIINVVKLNEIFRQAQESMIIVNAHKINNGEPLYLNTKGKD
                     FFFIRKSTNEEILNEIIGLVNERLPKFYKVDKLKDIQVLSSMRKGELGVTNLNIELQK
                     YLNKKEKFKVEENFSKRLFRVGDKVMQVKNNYTKKWETEDQKESGEGIYNGDIGYVYH
                     IDKDKKTIYILFDQTKIVSYLYDELDEIDHSFCTTIHKSQGSEFPVVVLPIAWAPPML
                     LSRNLLYTAVTRAKKLVVLVGDVKYLEYMIKNNRVNQRYSNLGYKLNKFKQEGLLIE"
     gene            complement(20785..21882)
                     /locus_tag="PC471_001119"
     CDS             complement(20785..21882)
                     /locus_tag="PC471_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2788257.1"
                     /translation="MSGKVNIKTVISFAGAYVATVIGSGFATGQEILQFFTFYGYAGI
                     IGGIISMVLFSWFGAEVIDKGRELKLKEPIKIYQVYCGKYLGTFFEWFGPLFLFGTLV
                     IMIAGAGATLSEYYGLNPYVGRIGMAIVSLITVSLGLTRLSKILGNIGPIIIIFTLLV
                     GAISLFSNIDALSNAGNMVDSLNIKTATSNGYFSGVLYTCYNVIIVITFLTGMGASAV
                     NKKDAVWGGIVGGVALMAAAIMMNLALLSDIGNIYTKEIPALYLADKISPIIGILFSV
                     VLLLGIYTTAVPLLWSVTNRFVEDDHPKFKIITIVVSILACIGGLLPFDKLVGTLYPY
                     TGYMGILILLCMLYRRITKTEGYKENKSEIS"
     gene            complement(22197..23390)
                     /locus_tag="PC471_001120"
     CDS             complement(22197..23390)
                     /locus_tag="PC471_001120"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HDF2788258.1"
                     /translation="MARHLFTSESVTEGHPDKICDQISDSILDALLEKDPQSRVACET
                     TVTTGLVLVAGEISTSAYVDIPKLVRETVREIGYTRAKYGFDCDTCAVITSIDEQSGD
                     IAMGVDEGLESKTGEEIEEEIEKVGAGDQGIMFGFACNETPELMPLPISLAHKLSRRL
                     TEVRKTGLVDYLRPDGKTQVTVEYEGSKAVRVHTVLISAQHCETVSNDKIREDLINHV
                     IKEVIPAELLDEETKIYINPTGRFVIGGPQGDTGLTGRKIIIDTYGGYSRHGGGAFSG
                     KDPTKVDRSAAYAARYVAKNIVAAGLADKCEIELAYAIGIARPLSIFIDTFGTGKVSE
                     EKLVELVNKHFDLRPGAIIRDLDLRKPLYKKVAAYGHFGRTDIDLPWERTDKVEQLRK
                     DALGE"
     regulatory      complement(23527..23630)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            23819..24328
                     /gene="yyaC"
                     /locus_tag="PC471_001121"
     CDS             23819..24328
                     /gene="yyaC"
                     /locus_tag="PC471_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore protease YyaC"
                     /protein_id="HDF2788259.1"
                     /translation="MYLAKVNYSDDDVIQMLSSVLKTIINENTIVVCIGTDRAIGDTL
                     GPLVGTILKNSNFKYPVYGTLDNPIHALNIYESLDTIKNTHIQGNFLAIDACLGSQSN
                     IGNIQIREGPILPGKGVGKKLPQIGNYSIVGIVDKIDENNKLSFNNIRLSFILDLAET
                     IALALLVST"
     gene            complement(24439..24867)
                     /gene="fabZ"
                     /locus_tag="PC471_001122"
     CDS             complement(24439..24867)
                     /gene="fabZ"
                     /locus_tag="PC471_001122"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HDF2788260.1"
                     /translation="MLNIDEIKKLIPHRYPFLLVDKITELEVGKRAVGIKNITVNEPF
                     FQGHFPEYPLMPGVLIVEALAQVCGVAMMSVEENKGKLGVFAGIDKVRIKREVRPGDT
                     LTMEVEMTTLRKNIAKADAKAYVGEELVCKGELMFALVEK"
     gene            complement(24883..25902)
                     /locus_tag="PC471_001123"
     CDS             complement(24883..25902)
                     /locus_tag="PC471_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HDF2788261.1"
                     /translation="MAGADIGIDLGTANVLVYVSGKGIVLEEPSVVAIDKRTDSVLAV
                     GEEAKKMIGRTPGNIVAIRPLRDGVISDYDVTEKMLKSFIDKIVDKKGFGRFFMPRIM
                     VCVPTGVTEVEKRAVEDATRQAGAREVYIIEEPIAAAIGAGVDISQPNGNMIIDIGGG
                     TVDIAVISLGGAVVSESIKVGGDRFDDAIVKYMKKQHNLLIGERTAEKIKFEIGSAFK
                     REEEKYMKITGRNLITGLPSSITINSTEMLEALRECVEQIVVATHAVLEKTPPELAAD
                     IGDVGIIMTGGGSLLYGLDKIIEQRTGITVTIADEPLSCVAKGTGIALGSIDLLETGG
                     SFKRK"
     gene            complement(26099..26359)
                     /gene="spoIIID"
                     /locus_tag="PC471_001124"
     CDS             complement(26099..26359)
                     /gene="spoIIID"
                     /locus_tag="PC471_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation transcriptional regulator SpoIIID"
                     /protein_id="HDF2788262.1"
                     /translation="MRSHIEERAIVVAKYILEKNTTVRQTAKTFGVSKSTIHKDVTER
                     LKEINPSLAKEVKNVLDKNKSERHIRGGLATKLKYEKEHKKM"
     gene            complement(26457..27125)
                     /locus_tag="PC471_001125"
     CDS             complement(26457..27125)
                     /locus_tag="PC471_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HDF2788263.1"
                     /translation="MKKKLLEKDGFYLSLFVCVCLLAVGGVWFTNNNVDKLASNKGIM
                     ENANKDSEEEIHLIEKDKKDAIPTATDSKQNLEKAKSKEENKSSTTKLNYIGDKVIRG
                     YSEKEPSYSKTLDVWETHKGVDISCTKGKEVKSLLNGIVVDVFDDEEYGQSVKIKSDN
                     NIVVVYSNLDKNVSVKKEQKVTEGQSLGTVGSTSQIESEEGIHVHLEAYSGEKSIDPM
                     SLIK"
     gene            complement(27191..28255)
                     /gene="spoIID"
                     /locus_tag="PC471_001126"
     CDS             complement(27191..28255)
                     /gene="spoIID"
                     /locus_tag="PC471_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein D"
                     /protein_id="HDF2788264.1"
                     /translation="MKNPLVVLLGFVTCSVLVPSLITLVSYKNVELTEKPESPVSINK
                     TIKKSDIEDKGNKEEKNTVNYETVNKKAPIINVYNHITGKTEKMDMENYLCGVLAGEM
                     SSEFDIEALKAQSVAARTYVVYKQEHGKSSKHKNAVVCTDYKHCQEYKSYDTLKKLNG
                     EEWIKNKYSKIQEAVRGTKGQIITYNDKAILPLYFSTSSGKTENSEEVFSAKYPYLKS
                     VESPYDKYSPKFASTLKISNTDFVKSLRRAYSTIVIDVNNLSKQVSITKRSDAGTVEK
                     IKLGNKELTGKDIRTVFKLNSANFDIKFGEGYIDFVVKGYGHGVGMSQWGAEGMAEEG
                     YKYYDILSHYYTDTKIKDIY"
     gene            complement(28342..29595)
                     /gene="murA"
                     /locus_tag="PC471_001127"
     CDS             complement(28342..29595)
                     /gene="murA"
                     /locus_tag="PC471_001127"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HDF2788265.1"
                     /translation="MAKIIVKKSNPLKGSVKIDGAKNAVLPIIAATLLANGKSTLNGV
                     PNLRDVHVISDLLRHVGAEVEYKENTLTVDASNIKTCEAPYELVRKMRASFLVMGPLL
                     ARFNSTKISMPGGCAIGTRPIDLHLKGFKALGAKIEMDHGFVEAATEKLVGNKLYLDF
                     PSVGATENIMMAASLAEGTTIIENAAEEPEIVDLANFLNEMGADVKGAGTNTIKIKGV
                     KELKGAEHNVIPDRIEAATYMVAAAMTKGDITVENVLMEHLKPVVAKLREAGCEITEM
                     DNSVRVVGPKVLKPIDIKTLPHPGFPTDVQAQFMAMLTVANGTGVVIETVFENRFMHV
                     AEFNRMGANIKIDGRSAVVNGVDELHGAAVNATDLRAGAALILCGLIAEGETQIGEIY
                     HIQRGYVDIDKKITALGGQIEIVED"
     gene            complement(29616..30353)
                     /locus_tag="PC471_001128"
     CDS             complement(29616..30353)
                     /locus_tag="PC471_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YwmB family TATA-box binding protein"
                     /protein_id="HDF2788266.1"
                     /translation="MKKVKLAISFFILMIIGMTISYADTSINTRYNRLSKALEISEAN
                     FKFYNVKINSVINHNLSNKEFHNIFIEIINNLKLDKKGINWNQKGSGDKIQLCATIKG
                     SDKEISIKGINKNNKESYIVVDILDNKVYKHKEVIYSNVENVLNKYSSQVDVSACIVG
                     EYTKRLQNNKYDDILQKILYNMSAKQIDEVKDVNFLSITAYSKVLNDYELEYLGEKIN
                     LNIGIKYNEDDGKTLIYIATPIIKLDY"
     gene            complement(30816..32111)
                     /locus_tag="PC471_001129"
     CDS             complement(30816..32111)
                     /locus_tag="PC471_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase"
                     /protein_id="HDF2788267.1"
                     /translation="MTNDFKKEKKEDYFVNLKAISTEVLQEKVQDNAWVIVDTRLNDA
                     YNGWKLDGVKRGGHIKGAVDFSANWLSVYSDRKDEVLEQALKTKRIDLDKNIVLYDAN
                     GKDALVVADYLSKKGYKYLYKYDIKQWADDENLPMERYKNYQMIVPAFIIKDILDGKI
                     PETFEDSKNIKMIEASWREESYTKGHIPTSVHVNTDIIEPPPTWMLDNDDNLTKFALD
                     YGLTKDDTVIVSSSTPMASYRLAVILRYIGVKDVRVLNGGTNSWLSAGYELEFISNPK
                     HSCTNFGADIPVNSQLIVTTSELRQKLKEKNKFILVDNRTWDEHIGKVSGYTYYDKKG
                     RIPGALYGHSGSDSVSLEEYRNIDNTMRNKYEILEMWDKENIDVNKQLIFMCGSGWRA
                     AEVLTYANVIGVENTSLYSDGWMGWSLDNSNLIEVGEHK"
     gene            complement(32562..34394)
                     /gene="glmS"
                     /locus_tag="PC471_001130"
     CDS             complement(32562..34394)
                     /gene="glmS"
                     /locus_tag="PC471_001130"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003401499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HDF2788268.1"
                     /translation="MCGIVGYLGSRKAAEVIVEGLSKLEYRGYDSAGVAVNSSNEKEL
                     NIRKFKGRLSVLAEDLEKNPIDGNLGIGHTRWATHGEPSDVNSHPHFNQAKTIAVVHN
                     GIIENYMEIKEELISEGVKFESQTDTEVIAHLVDKYYEGNLLDAVYKTISKLRGAYAL
                     GVICKEHGNELVAVRKDSPLVVGVGEGENFIASDIPALLKYTRDVYFLENGEVVHLKD
                     ENVTVYDSNRNLVEKEVFHVTWDVEAASKGGYDYFMSKEIHEQPTGVRETLERRLDDN
                     GNIILDSINISKEDLEKINKVYIVACGTAYNAGLLGKYAIEKFVNIPVITDIASEFRY
                     SDPFVDENSLVILVSQSGETADTLAVLRDSKAKGARILSITNVVGSSIARESDDVFYT
                     WAGPEVAVASTKAYTTQITSLYMIALDFAIKKGTITREFYDSMISKMKEIPSKIQEIL
                     DNEEYIKEVAKTVVSSEHAFYLGRGIDYSLAMEGSLKLKEISYIHAEAFAAGELKHGT
                     IALIEKGTPVIAIATQEKLFEKMVSNMEEVRARGAYVVAIAQSHNKDVEKAADKIIYI
                     PNSDDILSPILAVVPMQLLAYHVSVLRGCDVDKPRNLAKSVTVE"
     gene            complement(34661..36007)
                     /locus_tag="PC471_001131"
     CDS             complement(34661..36007)
                     /locus_tag="PC471_001131"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HDF2788269.1"
                     /translation="MRKYFGTDGVRGVANTELTCDLAYKLGRAGGFVLAQGDHRVKVV
                     VGKDTRISGDMLEASLIAGLMSVGCDVITVGIIPTPAVAYLTRKYGADCGVVISASHN
                     PVEYNGIKFFNKNGYKLDDEIELKIEEYIDDIDKIDCLPIGENVGRKLHEHCAQRDYV
                     DYLKSIISTDFKGLKVVLDCANGASYKVAPIVFDELGASVISINSSPDGNNINYKCGS
                     THPEQLQRAVLEHNADLGLAYDGDADRLIAVNEKGQIVDGDHIMILSALNLKKNNKLA
                     QDTLVVTVMSNIGLTIAAKENGINLSTTAVGDRYVLEDMVKNGYNLGGEQSGHMIFLD
                     YNTTGDGVLSSLILANIILQEKKPLSEIASIMSQYPQVLVNATIKNENKNKYMEYPEI
                     KTEIERIESILDGNGRVLIRPSGTEPLVRVMLEGKEEGQIKELATNLANLIQEKLS"
     gene            complement(36177..36734)
                     /locus_tag="PC471_001132"
     CDS             complement(36177..36734)
                     /locus_tag="PC471_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HDF2788270.1"
                     /translation="MSTARVICAGFGGQGVMSMGQLLTYAGMLEGKEVSWLPSYGPEM
                     RGGTANCAVTVSNEPVGSPLITDDATAAILLNIPAFEKFKDDVIPGGKIIVNSSLIKE
                     KVDRTDVDVYYIPANELAAELGNDKVANMIMLGAYLKVSDTVDIESVLEAFKKVFGPR
                     KEKFVPLNREALQKGMDATCSKATN"
     gene            complement(36735..37487)
                     /locus_tag="PC471_001133"
     CDS             complement(36735..37487)
                     /locus_tag="PC471_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate oxidoreductase"
                     /protein_id="HDF2788271.1"
                     /translation="MAVVFKKTEGLQDTQTHYCPGCTHGIIHRLVGEVLEELGVLGDA
                     VGVVPVGCSVLGYKYFNCDTQEAAHGRAPAAATGIKRVHPENTVFTYQGDGDLASIGT
                     AEIVHAAARGEKITTIFVNNTTYGMTGGQMAPTTLVGQRATTAQSGRNAETQGYPIRV
                     SEMLATLTGAVFVERVAVDTPAHVRQAKKAIKKAFQVQQAGLGFGIVEVLSTCPTNWG
                     LAPNDALQWLRDNMIPYYPLGNFKNVEVEEVK"
     gene            complement(37487..38566)
                     /locus_tag="PC471_001134"
     CDS             complement(37487..38566)
                     /locus_tag="PC471_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HDF2788272.1"
                     /translation="MAKILMKGNEAFGKAAIEAGCKYFFGYPITPQSELPEYLSRELP
                     KIGGAFVQAESEVSAINMVYGGAGAGARVMTSSSSPGVALKQEGITYAVGAEVPCVVL
                     NVMRGGPGLGSIQPSQADYFMSTRGGGNGDYRTPVFAPATVQEAVDMIMEAFDVADYY
                     RSPVMVVADGMIGQMMEPVEFRAPEKKRELPPKDWATVGTKGKRKPNVINSLYLEPEV
                     LEDHCWHLQEKFDAMEKNEVQYEMYKTEDAEFVFAAYGTTSRVVKSAIDILREEGIKA
                     GLIRPKVLWPFPFEAFNQIPNARNILTVEMSMGQMVEDVKMAVEGKLPVYFHGRPGGM
                     TPTPAEIVEKAKKIIAGELVAGGAR"
     gene            complement(38595..38810)
                     /locus_tag="PC471_001135"
     CDS             complement(38595..38810)
                     /locus_tag="PC471_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HDF2788273.1"
                     /translation="MAKGKVSFNQERCKGCGLCVEACPVKIIQLDSNVINKKGYNPAT
                     VFEMEKCIGCASCATMCPDVVITVERD"
     gene            complement(38853..39530)
                     /locus_tag="PC471_001136"
     CDS             complement(38853..39530)
                     /locus_tag="PC471_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDF2788274.1"
                     /translation="MITNDKRIRIITGHYGSGKSEFAMNYVVKLRDMVSGKVAIADLD
                     VVNVYFRTREKKELMKSLGIQPIDSSINAPTLDLPAVSAEVMSPMVDHSYNSVIDLGG
                     DNVGARVIGRFSHLLKEGDYDMLFVINANREKTQTSEEVIQYIKEIEKSSKLKVTGLI
                     NNTHLIRFTTIDDVLRGQKVAKEVSEKCNIPIRYVACLENLVEQLPKDLEGEIFPIKL
                     YMREDWM"
     gene            complement(39520..40599)
                     /gene="buk"
                     /locus_tag="PC471_001137"
     CDS             complement(39520..40599)
                     /gene="buk"
                     /locus_tag="PC471_001137"
                     /EC_number="2.7.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="butyrate kinase"
                     /protein_id="HDF2788275.1"
                     /translation="MSKIFKILTINPGSTSTKIAVFDNEDLVFEKTLRHSSEEIGKYE
                     KVSDQFEFRKQVIEEALKEGGVKTSELDAVVGRGGLLKPIKGGTYSVSAAMIEDLKVG
                     VLGEHASNLGGIIAKQIGEEVNVPSYIVDPVVVDELEDVARISGMPEISRASVVHALN
                     QKAIARRYAREINKKYEDINLIVAHMGGGVSVGAHKNGKIVDVANALDGEGPFSPERS
                     GGLPVGALVKMCFSGKYTQDEIKKKIKGNGGLVAYLNTNDAREVEERIEAGDEKAKLA
                     YEAMAYQISKEIGASAAVLKGDVKAILLTGGIAYSKMFTEMIADRVKFIADVKVYPGE
                     DEMIALAQGGLRVLTGEEEAQVYDN"
     gene            complement(40621..41523)
                     /gene="ptb"
                     /locus_tag="PC471_001138"
     CDS             complement(40621..41523)
                     /gene="ptb"
                     /locus_tag="PC471_001138"
                     /EC_number="2.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate butyryltransferase"
                     /protein_id="HDF2788276.1"
                     /translation="MRSFEEVIKFAKERGPKTISVACCQDKEVLMAVEMARKEKIANA
                     ILVGDIEKTKEIAKSIDMDIENYELIDIKDLAEASLKSVELVSQGKADMVMKGLVDTS
                     IILKAVLNKEVGLRTGNVLSHVAVFDVEGYDRLFFVTDAAMNLAPDTNTKKQIIENAC
                     TVAHSLDISEPKVAAICAKEKVNPKMKDTVEAKELEEMYERGEIKGCMVGGPFAIDNA
                     VSLEAAKHKGINHPVAGRADILLAPDIEGGNILYKALVFFSKSKNAGVIVGAKAPIIL
                     TSRADSEETKLNSIALGVLMAAKA"
     gene            complement(41827..43008)
                     /locus_tag="PC471_001139"
     CDS             complement(41827..43008)
                     /locus_tag="PC471_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2788277.1"
                     /translation="MINRLKNNTKIKIISFLSAIALWMYVMAVVDPEETKGFEDIPVS
                     ISNMSDLKDKNLVIYPDEKLTADVYIKGSLSKVQSVKKEDIHVYGTIEDPIQGQKEVY
                     LKANIPQGVTLEFKHDVLVVNLEKNIKEKRDIKVEVSGNSKRNINKIELSKEFVDVSG
                     PRTLVKEVKSVRANLDVGNETDDFSKKLQLIPLNSDGTKVEGVTLSSSFVTAKIELLK
                     EKIVPIRVSLNDNKDNDVLKNYKLSKEQVTIKGKKEDIDKISYIKTKPIDLADLANGE
                     EKEVSLEIPEGITLEDSSITIKLNDTKQLSAEFLYNAGDIELRNIPTNMDNPNVISDV
                     DIKVVVESMEDLSKLNKKDISLYIDLSQGPEEDSKYKIKYETTYQFKKINIEPNIVEV
                     E"
     gene            complement(43005..43844)
                     /locus_tag="PC471_001140"
     CDS             complement(43005..43844)
                     /locus_tag="PC471_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HDF2788278.1"
                     /translation="MLDINSFLNGFFNIILRMSIYDLIDISIVAYIFYKIFMFIKDTR
                     AEQVFKGIIFLLLATQLSNTFKLHTVYWISLKALDYGVIAALIIFQPEFRAGLEHIGR
                     AKFNLFGKNVNTSEETLNRNIEEIVEALYSLSRQKIGALIIMERETRISDIINTGTII
                     DAEISRQLLINIFIPNTPLHDGAVVIRDSKVKAAACFLPLTESKDLSKDLGTRHRAGI
                     GVSEVSDCITLIVSEETGGVSIAKAGKLYRDISRERMMNILRSNLKTNTETRSFFKGG
                     IFK"
BASE COUNT        13211 a         8451 c         4725 g        17987 t
ORIGIN      
        1 catattttaa tagttaatcg ttttaattct tcctttattt taaaatttaa atttgtctaa
       61 atatttctta ttttatatac attttttgat aaattaataa agccataata tatttaatat
      121 atcaatcaca atataggact gaagttataa ataaattatg gctattcaat acatttaatt
      181 ttatattttc ttgtctaatt atttctttat tatatttccc atttttttat aaagtggcaa
      241 tgttactaaa gatattataa cagccttcag cacattaaat ggagctatca tccaaattac
      301 aaaagtgaat aaatcatgaa cttgaggatt tatagctgaa cctaaaccta ttatagcatc
      361 tagtcccata agttgaccat aaagtggcaa cattacaaaa taatttacta atgaaccaat
      421 tatagtcata gatattgttc ctaatacaaa accaatcagt acacctttta aatctccact
      481 tctcttatat gaataacaaa gtatcattac ataagaacca ccaattaata tatttgcaaa
      541 ttctcctatt cctccagttg ttgttgctgt tattgcttgt aacacatttt ttactacaac
      601 tattccaaat ccaactaatg gtcctagaga cattcctccg aatattgctg gaatatctga
      661 taagtcaatt tttaagaaat ctggaaatat acctggtatt ggtgcttgaa taaacattaa
      721 taaataagat attgcagata aaattgacat tactactaaa gttcttgttg aaatcatatt
      781 gcctcttttt gctgtgtttt gcataattat tttcccccta atcaatatgt ttatattttt
      841 taggaaaaac aaaaaactcg aagaccctat cttcgagttt ttatggatat agtttaaaca
      901 aactaaaata aacttattta tcttccataa atatcttctc tcatccagac tttactgtcg
      961 gctttggaat ctcaccaaat caaccaccta agtagttcgc gggctatacc gccggtaggg
     1021 aatttcaccc tgccccgaag atcttctttg attttttaat tttccttaca ctcttatatt
     1081 agttaatttt ttaataattg tcaagttatt ttttctcata cactactttg ccatttatta
     1141 tagtgtaaag tacattactt tgaatctcta gtggagaatt atcccaaatt actaggtctg
     1201 catctttacc aacttctata gagcctactc tatcttctat tcctaaagcc ttagctggat
     1261 ttatcgtaat tgattctatt gctttttctt ctttcatacc atgttttact gcaatacctg
     1321 cacatattgg aagatattga actggtatta ctggatggtc tgtcattata catacatcaa
     1381 gacctgcatt tgaaagtatt cctgctgtat taaatgtcaa atttctaagc tcaaatttag
     1441 acctttctga caaagatggc cctactatta caggaaaccc ttcttccact aattcatcta
     1501 caataagatg accttctgta caatggtcta atgtaagctt taaattaaat tcttttgcaa
     1561 ttcttattgc tgtaaacata tcatctgctc tatgagcatg tgccttaaaa ggaatttctc
     1621 ttcttaaaac tggtattaaa ctttccattt ttatatcata ttcaggtttt tcacagtctt
     1681 catcatcatg actctcatac atatcaatat cctctaaata ttcttctgct ttttttaggt
     1741 tttctctaag taaagcagct atagccattc tagtttgagg agatttatca tcctgaccat
     1801 aacagctctt tggattttct ccaaaagcaa ttttagatgc aactggattt tttattacca
     1861 ttttatcaat tctttttcca catgtcttta tagctataca ttgaccaccc ataacatttg
     1921 cacttccagg ggttgtacaa acagaagtta ttccaccttc aaaagcttct ttaaaagttc
     1981 tatccattgg attaattcca tctataggat ttaattgtgg tgttattggg tcagtctctt
     2041 catttccatc tgctccttca aaccctatac catcttccca taaacctaag tgagtatgag
     2101 catctataaa tccaggaaac acaagtttat tactagcatc tattacctct acatctaaag
     2161 gagctatcaa atcttctcca atttctatta tttttccttc atctataagt atatctccat
     2221 gaattatccc atttgttatg gtatttattt taccattttt tataaaaatc attcattttc
     2281 ctcctgattt tcaagactat cgttcttatt ttaatatatt ttcttactat atacttttac
     2341 aaatttttaa catttataca ctcgagtaat cttatttaca aatatagaat ctaagtacta
     2401 taaccataca taaacttatc tattttttca ttgataaggc aactcttttt ttgtttaagt
     2461 caattccaat gacttttaca tctactatat cacctactgt aacaatactc atagggtctt
     2521 tcacaaaact attactcatt tctgatttat gaactaatcc gtcatttttt attccaatat
     2581 ctacaaatgc accaaagtct acaacatttc ttattgtacc ttttaaagtc ataccctctt
     2641 gtatatcttc aatttttaag acatcagttc taagtatagg ttttatacct tcttctcttg
     2701 ggtctcttcc tggttttttg atttcagcta tgatatcttt taacgtaact tgtcctactt
     2761 ccagtttttc acttaattcc ctcaagccaa tttcttttat tttttcatct atttcaccta
     2821 tattcttatt ctttacatca tctaaagaat atccaattat ctcaatcatt tttttacata
     2881 tatcataact ttcaggatgc acacctgtat tatctagtgg atttttaccc tctaagattc
     2941 tcataaatcc agcacattga gtaaatgctt gaggtcctag tctttttact tttttcaatt
     3001 gtgctcttga cgtaaaatct ccattttctt ctctatatgc tattatattt tttgctatag
     3061 ctttacttat tcctgcaaca tgttctagta gggagtatga tgcagtattt aaatctactc
     3121 caacactatt tacagaatct tcaacaactc catctagtac ttcttctaat ctctttttgt
     3181 ttaaatcatg ctgatattga ccaacaccaa tacttttagg gtctattttt acaagctctg
     3241 ctaatgggtc ttgtaatctt ctagctatag atatagctcc tcttattgat acatttatat
     3301 ctggatgttc ctcatttgca agttcagatg ctgaataaac agatgctcct gcttcactaa
     3361 ctatcacata ctgcacttcg ctatctattt cttttatcat ttctgataca aaagtttctg
     3421 attctctaga tgcagtacca tttccaattg aaattatatc tatatcgtat ttttcaatca
     3481 atccttttaa aactttcttt gctccctcta catcattttg agggtctgtt ggatatactg
     3541 ttgtataatc tagtagttta ccatttttat ctactacagc tattttacat ccagttctaa
     3601 atgcagggtc aaatcccata actactttgt ctttaactgg tggttgaagc aataaacttt
     3661 taacattctt tccaaataca cttatagctc tttcttgagc tatttctgtt aaatgatttc
     3721 ttatttctct ttctattgat gggaaaatta atcttttata agaatcttct attgaactta
     3781 cgatttcttc tttatttttg aaatttttat catttacata ttcgtttatt atgtaattta
     3841 aaactttatc attatttatt tccaacttaa cctttaggaa gctttctttt tctcctctgt
     3901 ttattgccaa aactctatgt ggagccatac tttttactgc ttcgctataa tcataataca
     3961 tatcataaac cgatttctca tcagtagcac ttttgctaac tatcatacct tctcttaaag
     4021 ctaattctct tatatacttt ctaatcttag catcatctga gactagttct gcaattatat
     4081 ctctagcacc ttttagagca tcttcaatag aaagaacttc tttctcttca tcaagataat
     4141 tctttgcctc tatttctata ttatctaaat tattctctag tattgacaat gctaaatttt
     4201 ctaatccttt ttcttttgct attgttgctc tagttctttt cttttgttta tatggagcat
     4261 atatatcttc tacttcttgt aatgtttttg ctttttctat attttgtgtt atttcatctg
     4321 ttaatttccc ttgttcatct attagtctaa ctacatcatc ttttctactt tgaagatttc
     4381 tcaaatacat taatttttca taaaattctc ttagtgtaac atcactcatt tcgccagtca
     4441 tttcttttct atatctagct ataaatggta ttgtatttcc ttcatcaata agttttaatg
     4501 tattatttat ttgttcatct ctaagattaa actctttttt taagatttga tttatatcca
     4561 atttattcac tcctaatgtt agtttttcat aagtataaca aaacagaacg aaggctgagg
     4621 gttatcgtcc tcagcctaag tgctgtaagt gcatattaag cactaacctt cttatacata
     4681 tatcttaatt acagtgcata tttattaaac tattttattc atttttaagt attcattaat
     4741 aaacaagtct atatttccat tcattacact atctacattt ccaaactcag catttgttct
     4801 atggtcttta accaatttat atggttgaaa aacatatgac ctaatttgac taccccaagt
     4861 tatttgtgag tatttgcctt gtatatcttc tattttttcc ttttgctcta actcttttaa
     4921 ttctatcaat ttcgccatta aaagtctcat agctctgtcc ttatttagat gctgagacct
     4981 ttcattttgg cactgaacta ctacaccagt agggatatgt gttattctaa ctgctgaatc
     5041 agtcgtattt acatgttgcc ctccagctcc agaagctcta tatgtgtcta tctttaaatc
     5101 tgatggatta atttctactt ctatattttc atctaactct ggtgttacat ctatagatgc
     5161 aaatgaagta tgtctttttc ctgatgggtc aaatggagat attctcacta atctatgaac
     5221 tcctttttca ctctttaaat acccataagc atttgttcct tctacaagaa ttgtagctgt
     5281 ctttatccct gcttcagggt ctgatattat atctaaaagc ttaactttat aacccttact
     5341 ttctgaccat cttatataca ttctcaaaag catttgagcc caatcttgag catctaagcc
     5401 accagtacca gcatttatag ataatatagc attattttta tcatactctc catttagaag
     5461 agtctttatt ttcatttcag ataatttttc ttccattgat tctatggact tttctatatc
     5521 tctttctaca gaatcatcat cttcttctaa acctatttca attaatactt ctatatcttc
     5581 taataagctt tttaagcttt catattcttc taatgtctct tttaaagatt tattttcttg
     5641 aagtactctt tgagcgactt cattatcatt ccaaaaatct tgttgattca tcttttcttc
     5701 attaatttgt atatttttca tcaatgaagc tatgtcaaag agaaaccctc aattcttcta
     5761 aatttgaagc catctgctcc atactatgtc tatactcttg taactttaac atttaatcct
     5821 ccgagattat cttccacaac agtttttata ttttttacca cttccacaag ggcataagtc
     5881 atttcttcca accttttcat cttttttaac agttttactt gttggaagtg ttccatctga
     5941 tggagaagat agattttcaa catctacaac tgcttttctt tccactggag tttctattgt
     6001 tatattaaat aaatatctta cagtatcttc tttgatatgt ttattcattt catcaaacat
     6061 atcaaaacct tccattttat aagcaataac tgggtcttgc tgacctacag cacgaagacc
     6121 aataccttgg cgtaattggt ccatagcatc tatatggtct atccaatggt tatcaacagc
     6181 ttgaagtaag attactctct caacttctct cattctctca tagccaactt gttcttcttt
     6241 tgaagtatat atcttcatag ctatttcata aactttttct gttatttcaa ctggattaag
     6301 tttttctatt tctggtattt ctatgctacc ttttggcaag aacagattat acatatgttc
     6361 tttgaatcct tcttcatcaa atcctttatc ttgtgtataa agagtaactg cttcttctat
     6421 tatagaatga gtcattgatt gtatttgttc ttgtaaatct tcaccttcta atacacgttt
     6481 tctctctgca tatattattt ctctttgttt attcataaca tcatcatatt gaagtacatg
     6541 ctttcttatt ccaaagtttt taccttcgac tttcttttgt gctccttcaa tagacttaga
     6601 taatattcta tgttctatcg gcatgtcttc ttcaagacct attttatcta caataccaga
     6661 tattctatca ctaccaaaca atctcataag gtcatcatca agacctatat agaatcttga
     6721 actccctggg tcaccttgac gtccagcacg acctctcaac tggttatcta ttcttcgaga
     6781 ttcgtgtctt tctgtaccta ttatagcaag tccacctgct tctacaactt gtttttgttc
     6841 ttcttgtgtt tgttgcttaa acttttcaaa tagtttttca tactcttctc tagcttcaaa
     6901 tagaatttca ttacctttta caggtatacc ttctattgga gtatctactc tatttactat
     6961 ttcttcttta aatccatttc ttctcatttc tctcttagtt aaaaagtctg gatttccacc
     7021 tagaactata tctgtaccac gaccagccat attagtagct attgtaacag ctccaagtct
     7081 accagcttgt gctattattt ctgcctcttt atcatgatgc ttagcattta atacctcgtg
     7141 ctttatgccc tttttcttta gtatttgtga taataattct gatttttcta ttgatactgt
     7201 acctacaagt attggttgat tgactttgtg cctttcaatt atttcttgtg ctacagcatt
     7261 aaatttacct atttcacttt tgtatacaca gtctggtaag tcttccctta tcataagctt
     7321 attagtaggt acttggaaaa catccatttt gtagattgct ttaaactctt cttcttctgt
     7381 ttttgctgta ccagtcatcc cagaaagttt tttgtacatt ctaaagtaat tttggaatgt
     7441 tacagtagct agtgttttag attctctttg tattttaaga ccttctttag cctctattgc
     7501 ttgatgtaaa ccttctgaat atcttctacc aaacataagt ctacctgtga actcatctac
     7561 tattactatt tctccatctt tagcaacata atcaacatct ttcttcatta ttacatgtgc
     7621 tcttaaagct tgatttatat gatggtataa ttctgtatga gatatatctg ttatattatc
     7681 aacattaaaa tacacttctg ctttttgtat acccgatgct gttagagata cagccttatc
     7741 tttttcttct aattcataat catctggttt taaggttaaa acaaatgtgt ttgcatctga
     7801 atataagtga gttgatttgt ctccaggtcc agatatgata agtggtgttc tagcctcatc
     7861 tattagtata gagtcaacct catccactat agcataattt aatcctcttt gaactctttg
     7921 ctctttgtgt attaccatat tgtcctttag atagtcaaat ccatattcat tatttgtacc
     7981 ataagttata tcacaatcat attgttcttt tctaactttt ggattttgac cgtgtataat
     8041 aacgcctaca ctcattccta aaaactcata gatttttgcc atttggtctc tatcacgttt
     8101 tgcaaggtaa tcatttactg ttacaacatg tacacctttt ccagtaagcg cattcaaata
     8161 aacaggagca gttgcaacca aagttttacc ttcacctgtt ttcatttctg ctattcttcc
     8221 ttgatgtagt actatacctc caatcatttg aactctgtag tgtctaagtc ctaacactct
     8281 cttagaaact tctctaacta ctgcaaaagc ttctggaagt atatcatcta tactttcacc
     8341 atttgctaat ctttctttaa atatatttgt catattttta agttccgagt ctgccataga
     8401 ttcaaattta ggttctaatg aatctattat atcaacagtt ttattgaact tttttaattc
     8461 ttttttatct gccatgttga ataaattatc cataaagctc atattttaca tctctcctcg
     8521 ttctttattg tactattatt cattttacca taaaaactat acttttaata ttaatttaaa
     8581 aagctatcga taatttattt atcgatagct aatatgatta aatctaaatt gctttattca
     8641 tgttctatta taccatatcc accattatta cgcttataaa ctactgctat ttcatctgaa
     8701 tctatgttct tgaacatata aaaatcatgt cctattaatt ccatctgtaa tacagcttct
     8761 tcttcactca ttggcttaac actaaatttc ttacgtcttt gaattactaa ttcttgattt
     8821 tcatccccat aattctcttg ttcatcagct agatttatct ctgcatcttg gaatcttata
     8881 ctcttgttgt ctttatgttt atcttgtaat ctctttttat atttttttaa ttgtttactt
     8941 aatttatcat atacaacatc tatagctgtg tataaatttt cctgcatatc ttctgctcta
     9001 attataggac catttacagt agctattgtt acttctattt tttgtcttgc ttttctcgca
     9061 cttactgtaa cttttacatc agtgtctgga tgaatgtaat tatctaactt acttagttta
     9121 gtttcgattt tattttttat tgcatcagtt aattcgattt gttttccaga tataattatg
     9181 ttcataaata catctccctt caattatgcc agtataagaa aatttatact ttaataatat
     9241 attctacacg aactctcata atcctctttt tattatttac ttttatgatt ttttctattc
     9301 aattgataga attatttatc atttttagtt acattgatac ttatgtggat taatattatt
     9361 acaaacacct gtggataatg tggataaaac tgttaataac tcttgttttt gtttatttaa
     9421 agctattttt actcctattt ctatccacaa aaatctccat taattgttta ttaatatgtg
     9481 aattcatgtc ataagatggt aattttagga aattatgtgt attatatgcc aatatacctt
     9541 tataattttt taataaatat aaaccctcta aaacctccat aatctatctt ttctttcatt
     9601 atttatattt aaaactaatt aaaagttata aagcatactc aacaataatt tcatatttaa
     9661 tacctattaa cattcatata cccaaattat aattaactta ctctctttat ttaaactaag
     9721 tattttatat tcatatacta tcttgaaatt atatttacga gttgtttaag cttttgtcta
     9781 tctatatctt caaaaggact gttatttatt ctaatcgctg taccaaaatg aaaattactt
     9841 gccttagtta attcctttat cttttctata ttattaaaat taagacctcc acctaaaaga
     9901 atttttatgt gattagaact aagcatcata ttctttatca tttgtatgtt atcaactata
     9961 cttcctttcc ctcctgatgt taatatattc gtaattttat cataatcctt caaaatttta
    10021 acactatcta ttgtatttga ttcatctatt gctctatgaa atgtaacatc aagattatca
    10081 caacatttca ataaaacatt tagatttttc tcatctatac tattgttttt atccaataca
    10141 ccaaatacaa ctccattagc accaatctct ttaaccacac ttatatcttt ttgcattata
    10201 cttatatctt ctttactgta tacaaaactt ttcgcatgat gtcttatcat tacgttcaca
    10261 ggtatcttta cactgttaac aacactttct ataagaccaa aacttggtgt caatccacct
    10321 tcagttaaag cactaactaa ctctattcta tctgcaccac aatcttctat aattttagcg
    10381 tcttctactg acattcctat tatttctaac ataactactc ctttgctaat tcatatattg
    10441 catttgcaaa tatctttgca tttaatatta tatcttctat atccatgtat tcatcaggat
    10501 tatgagctat tcctttttgt cctggaaatg aaggtccaaa cggaactata tttggcatta
    10561 cttttgcata cgtacctcca ttagttgtta cgggagttcc atctaatgat gtaacccttt
    10621 cataagctag ctgaagcttt ttaacaagat tactctcttt atcaaaatat acaggattaa
    10681 aattagaaat tacctctata tcgatactat ttggaaaact agcttttatt tcatccaata
    10741 catctttcaa tttataacta aaatcgtatt ttacctttat atctaatata gtatcactat
    10801 tttctatatt cagctcttta tatgtaacaa gcacattttc agacatattt tctataaaac
    10861 ctaggtatac atcttcttca taattgattt tactttttat tctaatcttt gccatgcctt
    10921 tttcaccata tacaacagga tatttacaat ctggtgtaaa tcccataata ggtgcttttt
    10981 ctttttctaa ataatatggt atatcttcaa atccactttc ttcatttgta ccaaatatta
    11041 ttctaactct cttatctaat tttaagttta attctttaat agcatataat ccatataagg
    11101 cagacattat aggtccttta ttgtctagta ctcctcgtcc ataaattctt ccattagttt
    11161 cttctccctt ataaggctga tgcttccaac catctccttc atgtacaaca tctacatgtc
    11221 caattacaca aacataatca tcactatctc catgctctgc ataaccaata taattatcca
    11281 agtttctaac cttaaatccc aatttttctg atatttctaa tgctttatta agtgcctccc
    11341 ctactttttc gccaaaaggg aagccatttt ctgactcccc tttgacacta ggtattttta
    11401 caatatctat aatatcactt aataaatctt cttttaactc atctatttta ttacttatga
    11461 tattcataaa aatctcctta ttataaaact ctattttaat tatgtaacca ctactttact
    11521 cacttacttc tattggtgct tttattctag cctcatatgc ttccatccac tcatttgata
    11581 taaccttatg tatagtcttt ccatctactc tatttgaaat atatacagta ggagattctt
    11641 ctttagttac tactgaaaaa gtaaaatcag ctatatttgt agctactccc cattcacctt
    11701 tattattcat ggcaactaat gataaatcac cagcttttcc tcttcttttg ataagctgtt
    11761 tatcaagttc atttactgca atatcacatg cttcttgtgg atgcttccct tctttcataa
    11821 gtctaactat ctcataagat atacatccct tcattaaatc ttctcccaaa ccagtagctg
    11881 tagctcctcc tatactacta tcaacataga atcctgaacc agatagtgca gaatctccta
    11941 ttctacctct tttcttcata aacaatccac ttgttgatgt agctgcacac atttttccag
    12001 ttttgtctaa agatatcata ccaacagtat catgaccagc atatgggctt aatcctttgt
    12061 ctaaagtctc tttttttctc tttttataat gtaacttcgc tctatcagtc aacatatttt
    12121 ttctttcaaa tccattttta tgagcataat cttcagcacc tattccaact aaaaaattat
    12181 ttaccttctc ataacttaac ttcctagcta tgctgacagg attagaataa tctcttatac
    12241 cagcaactgc gccaatatct aatgtgttac catccataaa tgcagcgtca agttctacct
    12301 cacaattttc atttggaagt ccaccatatc caaccgattt atagaatgga tagtcttcaa
    12361 ctcttcttac agcaagctct acagcatcac ctgcttccat gcctttttct aaatgttctg
    12421 atgcctcagt tattccttct agggccattc tccatgtcgc tatcattccc cacatgttaa
    12481 aattcccctc cttaaattat atagatttta ctaatgtatg cttattataa atagtataat
    12541 atttaaactt gctaataatt aatactctta ttgataacaa aactcttctt tcatcaaagt
    12601 tatatcttct ttatttgcta ttgcttttgc caatttacat atgttattta atgaatcatt
    12661 aagaccaatt ccaccttttt ttggagtctt aactatacat atgtcatttt tatattcgtt
    12721 tatcacatct atattttctt ctgacatagc tgcaaaagct ggtatatctg ttttatcatt
    12781 tatattttta gacagtattg ctgacatctt agaaaagttt tcgtaattaa agcttggtcc
    12841 acagattact acatcagggt ttaatttttt taccatagct atcatcttac tcgttacttc
    12901 ttcttgatta tttacaaaaa attcatcccc acaaaataag gtagctatta ccttagattc
    12961 atttaaaaat tgctccatca ttacacctgg tcctaatggg cttgattttc cagcaggagg
    13021 tatattactt ttctcttttc ctccagctcc agcttgtatt tggtccaata tcataactat
    13081 tttcttcata atcttcctcc tataatacta aatcatcaaa agatatatca tcatcttcct
    13141 cttcaacagt ttcattttct tctctcaagt attgcttatc tagcattttt ataaatgata
    13201 tatacataaa tattcctagt ataagaagta ctaattgtaa gactgcacct tgccatcctg
    13261 ttgttaaaaa accagagaat ataattggag ttgtccacgg taactgaact ccatttgtaa
    13321 gaggcactaa tcctgctgac atacagaagt aagctattat tatatttatt gttggaacta
    13381 ttgcaaatgg tatcaatagt attgggttaa gcattattgg tagaccaaat atcaatggct
    13441 catttatacc aaatattcct ggtaaaattg atagttttcc cattgacttt atacgtttac
    13501 ttttacatat taataacata gctatgacta atgatagtgt actacctgct ccaccaaatg
    13561 tcgcaaacat atcttggaat tgacctgtta ttatgtttgg tatttttgaa ccagcttgaa
    13621 aagcagctaa gttttcagct gatagaattt ttaatattgg attatacaca gccccaacaa
    13681 cactacttcc atttattcca aagaaccaga ataaatgtaa gaatatataa gcgattgcca
    13741 tagcacctaa actatttcct aattttaata atggcatttg taaaaactta aatataaatt
    13801 catgtatact tccatatggt gtagcttcaa aagctatctt aactaaaaag aaaactacca
    13861 taactattgc acttggtatt aatgctgcaa atgattttga tacagttgga ggaactccat
    13921 ctggcatttt caatgtccat ccttttttta caactgcagc aaataatttt acagaggtta
    13981 ttgctgttat catacctaca aacattccct tagaacccat ccaacctagt ggtatacctg
    14041 ttaccatgta aactgattct gttccttctg gtgtatatgg tattacaaat ggtgttatta
    14101 taaaaaaggc aactattgca actgctgctg atgctattgg gtctacatct atttgctttg
    14161 cataagaata tccaatccct aaaactgcaa gtagagtcat tacttcaaat gttgctacag
    14221 ttggttgttg caattttaca gcccaatctg gaccaaaaat ttgacctaca aattcattcc
    14281 atccttttat aggaaaattt gctactaata aaaataatga acctattatt aataatggtg
    14341 atgatactaa aaaaccatct ctaattgcaa ttaataattt atttttacct attttttcag
    14401 ccaatggcat aagtattctt tccaatctat tcatctatcc accctcattt cctattattt
    14461 tttactataa ttctacgctt gtttagattt tattagcttt atagcaagtt ttaatacttt
    14521 ttccccattc atagttccat aatcaacttc attgattata tctataagtt ttccttgagc
    14581 ttcaaacttt ggttttaact cctttagcat gtatttaact tgtggtccta ataatataca
    14641 atctggattt ttttcttcaa ctattttgtc aatttctgct atcggaaatg cctctacttc
    14701 tattggtaaa ttgtgttcat ctgctacctt ctgcatttta tctgcaagta aacttgttga
    14761 cataccaaag ctacaaaata aatatacttt tcttttcatt ttttaaatcc cccttaattt
    14821 ttttatttta atttttgaat ttcttttctt aacataatca tttcttttat aaggtttctc
    14881 tcactcatac aagtcattaa atggtcttga gagtgaatca ataaaataga tatatctgat
    14941 ttatctcccc cagcttcctt ttgtataagt tcagtttgta ctttatgtgc ttttaaaatt
    15001 tcttcatttg cattttcaat taattcatct gcttttttaa aatcgttttc ctttgcatag
    15061 tcaagtgctt cataaagcat agattttgca tttccagcat gaattataat ctttaaaact
    15121 atttcttcca ttttttcctc ctaagttgct tctctacttt tagattattt ataccttaca
    15181 aacatataat atcttataaa aacaaaaaaa catgcacaac tttttccatc acatggaaaa
    15241 ttttgtgctt atccatagtt cactatgaat tttatataag aatatctttt accttttcaa
    15301 tgtcacattt acttagaata tctagcatat tttcatcctc tgttaaatta gatatttctt
    15361 taaataattc tctaaatact tttctatcat catctttttt atacgctaac aaaagtataa
    15421 tctttatatc ctttccattt ctaattttta ttggtgtttt taaccttgca aaacataaaa
    15481 cagattgttt tatatatttc atgtctccat gagggaataa tattcctttt ccaacattgg
    15541 ttggatatga cttttctctt gttataatag attgtaaata ctctttttct gcataatgtt
    15601 tttcaattaa taaattagtt aaatatgtta tagcttcata aatatcatca tgttctaact
    15661 ctcttattaa ataatcagac atcattaatc tgttcataag tttatatgaa gtgtcttttt
    15721 cttttatttt gtttgtgaac tctttaagtt tattctcatc ctcatcagta aataatggtg
    15781 ttgttataaa aattggtttt tggatatttt catactcttg aaatgcaatt ataaaatcta
    15841 tagtcttatt aaagttatct ctttttaaat cttcctctct taatgtttca ataattttta
    15901 tgttatcaaa tctcttttct atctttactt ttaatagcat acttactcca aaactaaatg
    15961 gacatacaat acttgccttt ttatttgatt cccttttgct gtattttctt tctagagagg
    16021 tttgaaagtg aagtgtaaga taaccaattt catcttccgt tatttgtttt ccttttaatt
    16081 caatttctat tgaacttgat attactgtaa ataaaaaaca aaatttagtt tttatgttat
    16141 ctaaaagtgg attttctaag taaacattgc ttttcatttg atgcattgta acattcaaat
    16201 gacatattaa ttgttcataa agtaatatat cattattaaa atttattcct gattcttctg
    16261 atactaaatt tataattttt tttgtataac ttgctaattc ttcatttaga tataaagatt
    16321 tcttaatatt attactattt atttgtttgt tcatacccaa tattaaagat tctataaaca
    16381 atttttcttc ataatttaat actatggata aattagattt caattcatct tctatatcca
    16441 atataagcat ttttagctta agctctttct tagtatttga tacttttaaa gaatttccca
    16501 gccttattcg tattatattt ataagcaaaa atagcatcaa ttgcttgaat gatatatctg
    16561 taagtcttat atccatatcc tcttctatag taagcattat gttttttaca attattatat
    16621 ctactatatg gaagtactct atttcatcta tgtttaatct gtatttatct atatatttaa
    16681 ataagaatga tactaacatt attcttatgt ctttctcatc cccttcaata tatataccac
    16741 tgcctttttt agtcgagata tttaaattat aattatttaa tgctactgat aatttactta
    16801 tttcttctct gacaatattt ttatttatgt acaacttatt agataaatca tttaaagtaa
    16861 cttttttatt attacaaagt agtaaattta gtatctcaac ttgcttatga agctcactat
    16921 ttactctttt aacttcacct cgcaacttct gatttaccag tattttgtct gttccatctc
    16981 cactaaattt aatcccaata ttaggttttc ttttgatata agcattacaa aaagtagata
    17041 accattcatc aatatatttg caatcatttc ttattgtcct ttctgaacac tcaaaatcat
    17101 tggcaatctt attaacattt ataaactctt cttttaatag caatgttgtt aataaatttt
    17161 cttgtctttt attcaaacat atcacctctt tatgactcaa cctaaattta ttatatataa
    17221 aaaagagcta tacataaagg tatgctagct ctcttttaat atgatattta gctgacctgg
    17281 tttttgaccc cacactcgcc tactggaggg ttcgcttggg ttcgccgcac tactactctc
    17341 tctcgatttt attatcggta ggtcttactt ctaagccgca ccatgatcat taacccaatt
    17401 tttagtgtta acttacgtgg atccctttgc ctgcgactgg cttggacttt caaccacaga
    17461 cctaaatatc acttattcat tttaatattt taaactaaaa ttgtcaatat ataagattat
    17521 tccataatat aattatcatt tgcttttgta agtagagtca atacaaaaac cttattagca
    17581 cctgataact ttaaaagttt agaaatttcg tttgttgtta gaccagtagt aaatatatca
    17641 tctattaaaa gtatattttt attatttatt aatttaacat tttctttaac aacaaataca
    17701 ttttttaatt cttcttttcg ttctttctta ttcaatttat atagcattct tgtatatttt
    17761 tttctactta tgcaatctaa taatggaata tttactattt tacttaaatt aaatgctatt
    17821 ttttgtgcct gattaaaacc tcttttattt agcctttttt tatgaagtgg aacaaataat
    17881 atataatcaa attttatgtt ttctaaatat aatttttctt tcattatttg agctatatac
    17941 ttagccataa aggttttttg gttatacttc agccctaaca ccatcttttt tgtcacatca
    18001 ttgtattcaa tacaagatat tgatttatca aaataaaagt ctttattaaa acaataacta
    18061 cactcttcaa taaattcttt ttcaattgaa tgacgtatta ttggttttcc acacttcatg
    18121 caaccatctt gtataaaatt catttcttta aaacaacttt tacatattga ataagtattt
    18181 gttttcttta tggatttatc acaaattatg caacttatat tctcaggata tataaaatcc
    18241 aaacatttat taatcatatt tttaaaattc ttaataaaat tatcattgca tattttcata
    18301 tttattctat caacaaccct tcttgtttaa atttatttag cttatatcct aaatttgaat
    18361 acctttgatt aactctatta ttttttatca tatattcaag atatttcaca tctccaacta
    18421 atacaactaa tttttttgct cttgttactg ctgtatataa taaattacgg ctaagtaaca
    18481 taggaggagc ccacgctatt ggcaatacta caactggaaa ttcacttccc tggcttttat
    18541 gtattgttgt acaaaaacta tgatcaattt catctaattc atcatatagg tatgatacaa
    18601 ttttagtctg gtcaaataat atataaatag ttttcttatc tttatctatg tgataaacat
    18661 atcctatatc tccattatag ataccttctc cactttcttt ttggtcttct gtttcccatt
    18721 tttttgtata attatttttt acttgcatta ccttatcgcc aactctaaat aatcttttag
    18781 aaaaattttc ttcaacttta aatttttctt ttttgttaag atacttctgt aactctatat
    18841 ttagattagt aactcccaat tcaccttttc tcattgaaga taaaacttgt atatctttta
    18901 atttatcaac tttataaaat ttaggtaatc tttcattaac caatcctatt atttcattta
    18961 aaatttcttc attagttgat tttcttataa aaaagaaatc tttacctttt gtatttagat
    19021 aaagtggttc tccattgttt atcttatggg catttacaat tatcatactt tcttgcgctt
    19081 gtctaaatat ttcatttaat ttaactacat ttatgatatt agaattaatc atatccttaa
    19141 gtacatttcc agcacctaca gaaggtaatt ggtcactatc tccaactaaa attactcttg
    19201 ttcctaactt tatagctcta agtaaattgt acattaagat aatgtctacc attgatacct
    19261 catcaacaat tattacatct gaatttattg ggtcttcttc atccttaaaa aaagttaaat
    19321 catcatctgt tgaaaatccc atttctagca acctatgtat agtttttgct tctttattag
    19381 aagtttcact cattctcttt gctgctcttc ccgttggagc tgccagagtc acacttttac
    19441 cattattttc aaatatatct atgatagcat taatggtagt tgttttacca gttcctggtc
    19501 caccagttat aattaatact ccactattaa cagattcttt gactgctaaa atttgttttt
    19561 ctgctaactt aattttctta tcttcttcta aaacattaat ctcgttatct atatctattt
    19621 ttaagtcttc aaattcatac tgtgataatt ttactatttg actacatact ccattttcag
    19681 ctaaatagta tggcataagg tatataagat tttcattgtt tactttctct atatgtattt
    19741 tttgattata aactagcatc attacacact cttttattat ttctccattt agctccaata
    19801 atttttccga atcttgtatt agaacatgct ctggtaaata tgtatgccca ttacttaaag
    19861 atttatttaa agtgtataag attccctgac aaattctatc tttagagttt ttatctatac
    19921 caattttact tgctatacta tcagctactt taaatccaat tcctcttata tcttcagcaa
    19981 gttggtatgg gtttttattt ataacttcta ttgcactgct tttatatttt ttatatattt
    20041 ttaggcagta atttggtgtt attccaaaag gagatagctg tattattata tttctcaatt
    20101 ctctgtcttc ttcataactt tttactattt gttttacttt tttactccct ataccttcta
    20161 cttcttgtag tttttctggt gaattttgta tcacctctaa cgtatctaca ccaaacttat
    20221 caattattct ttttgccatt ttttcaccaa taccatgtat cattccagaa gataagtaaa
    20281 cataaattcc ttctaaggat gatggtgtta ctggcataaa actattcact tcaaattgag
    20341 tcccataaat cttatgattt acccactttc cttcaacttc tatgctttct ccaattgcta
    20401 aagtaggcat acatcctaca accacaattt catcattttc atttaccagg tgagctattg
    20461 tataaccatt atcttcgttt ttaaagacta tttcacttat cattccttct aatttttcca
    20521 tttttatctc ctaaaaatac taataatcgc tccatttaaa tgttagtata attttatata
    20581 ttaaaactat gataatcgta gaaaagtttt atatttttat acttaacgta atttccaact
    20641 attatacatt ttatcagaaa caaaaaaaat ctgcatccat aaaatatggt accatgaaat
    20701 acaggtacca tatttaaata gctattttat tttaattact ctatatttag agtataaatt
    20761 aaatttcata agtatattac agtactaact tatttcagat ttattctcct tgtacccctc
    20821 agttttagta attcttctat aaagcataca tagtaaaatt aaaattccca tgtaacctgt
    20881 atatggatat agtgtaccta ctaacttatc aaatggcagt aaacctccta tacaagctaa
    20941 tatacttacc actattgtta taattttaaa cttagggtgg tcatcttcta caaatcggtt
    21001 tgttactgac cataacaatg gtacagctgt agtgtatatt cctagtaaaa gtactactga
    21061 gaataaaata cctattattg gagatatctt atcagctaag taaagtgctg gtatctcttt
    21121 tgtgtatata tttcctatgt ctgaaagtaa tgccaaattc atcattatag ctgctgccat
    21181 aagtgctact ccacctacta taccacccca tacagcatct ttcttattta ctgcagaagc
    21241 tcccattcct gtcaagaatg ttattactat tattacattg taacaagtat aaagtactcc
    21301 cgagaaatat ccatttgatg tagcagtttt aatatttaaa ctatcaacca tatttccagc
    21361 atttgataaa gcatcaatat tgctaaataa actaattgct cctactaaaa gtgtaaatat
    21421 tattattata ggtcctatat tacctagtat tttagaaagc cttgtcaaac ctaatgaaac
    21481 agttattagg gaaacgattg ccataccaat cctacctaca tatggattta atccatagta
    21541 ctctgataat gtagctcctg ctcctgcaat cattattact aatgttccaa ataaaaacaa
    21601 tggaccaaac cattcaaaaa atgttccaag atattttcca caatatactt gataaatttt
    21661 tattggctcc ttaagtttca attctcttcc cttatctata acttctgctc caaaccatga
    21721 aaatagtacc atagagatta ttccacctat aatacccgca tatccataaa acgtaaaaaa
    21781 ttgcagtatt tcttgaccag ttgcaaatcc agaacctatt acagtagcaa cataagctcc
    21841 agcaaaactt ataaccgttt taatatttac tttgcctgac atacactact ccccccaatt
    21901 aaaattattt ttaaagtgta agtttattaa gattatatta gactcattat tgtgatattt
    21961 caatatattt ttaattattt aacattagtt aacacattct taaccatcct ttaacatata
    22021 tcccataata tgtataaatt tagtagagta ttatataaaa tgtattgacc agattaaata
    22081 ctgtttattc attatatgat gataaattaa catttattaa aaatatttaa ctcataataa
    22141 aaaagctgtt agaaagtaat cgtaaaatta cattctaaca gctttttagt taaaaattat
    22201 tcacctaaag catcttttct taactgctca accttgtcag ttctttccca aggtaaatct
    22261 atatcagttc taccaaagtg accataagca gcaacctttt tataaagagg ctttcttaaa
    22321 tctaagtctc ttattatcgc tcccggtctt aaatcaaagt gtttatttac taattcaaca
    22381 agtttttctt ctgaaacctt gcctgtccca aaagtatcta taaatataga taatggtcta
    22441 gctataccta tagcatatgc aagttctatc tcacatttgt ctgcaagacc agccgcaaca
    22501 atattttttg caacatatct agctgcgtaa gcagcagatc tatcaacttt tgtagggtct
    22561 tttcctgaga aagctcctcc accatgtcta gaatatccac cataagtatc tattattatt
    22621 tttcttcctg taagacctgt atctccttgt ggtcctccta taacgaatct tcctgttgga
    22681 tttatataaa ttttagtctc ttcatcaagt aattctgctg gtataacttc cttaataaca
    22741 tgattaatta aatcttctct tattttatca tttgatactg tttcacaatg ttgagctgat
    22801 ataagaactg tatgtactct tacagcttta cttccttcat attcaacagt aacttgagtt
    22861 tttccatctg gtcttaaata atcaactaaa cctgtttttc taacttctgt aagtcttctt
    22921 gataatttat gtgccaaaga tatcggaagt ggcattaatt ctggagtctc attacatgca
    22981 aatccaaaca ttataccttg gtctccagct ccaacttttt ctatttcttc ttctatctct
    23041 tctccagtct tactttctag accctcatca actcccatag ctatatctcc agattgttca
    23101 tcaatagaag ttataactgc gcaagtatca caatcaaatc catattttgc tcttgtatat
    23161 ccaatttctc ttactgtttc tcttactaat ttaggaatgt ctacataagc agatgtactt
    23221 atttctcctg ctactaaaac taaaccagta gtaactgtag tttcacaagc tactctagat
    23281 tgtgggtctt tttctaataa tgcatctaat attgaatctg atatttggtc acatatctta
    23341 tcaggatgtc cttctgtaac tgattctgac gtaaataaat gtcttgccat ttttattccc
    23401 ccttatttat tcttaatttt tattataggt tttaccttgt ttttaatttt agaaatttac
    23461 tcaaaataaa aaaacctctc ctttataaga agaggttaat aatcgtaatt acatactaat
    23521 taactgacct catctttcaa gacttttgtc ttgtaggaat tagcaccttt tcccatttgg
    23581 gtggttgctg ggcttcacag ggcctatccc tcagcctctc ttgataaggt aatcaagatt
    23641 taagttttgt tttactttac tagcttacta tttttttaaa attttgtcaa tatttttagt
    23701 catatttttt ctattccttt ttttgtaaga caaataaaat tttctatcta tctgaaattt
    23761 aaagttaggt ctttttaatg ctttttattc atatattttt ttgaaggggg tgttcctatt
    23821 gtatttagct aaagtaaact acagcgatga tgatgttata cagatgctaa gttcagttct
    23881 aaaaactata attaatgaaa atacgattgt tgtttgcata ggaacagaca gggctatagg
    23941 tgatacttta ggccctttag ttggaacaat acttaagaac agtaacttta aataccccgt
    24001 ttacggtaca ctagataacc ctattcacgc cttaaatata tatgagtctt tagatactat
    24061 aaaaaataca catatacaag gtaacttctt ggctatagat gcttgcttgg ggtcgcagag
    24121 taacataggt aatatacaga ttagagaagg acctatcctg cccggaaaag gtgttggtaa
    24181 aaaactacct caaataggaa attattctat agtaggaatt gtagataaaa ttgatgaaaa
    24241 taataaactt tcatttaata atatacgcct tagctttata ttagatttag ctgaaacaat
    24301 agccttggct ttattagtat ccacctaatc taaatataaa ctaaatgata aaattcttta
    24361 tataaaaagg tagatatttt agagtatctg attaaatact ccaaattatc taccttttta
    24421 cttttatatt tcaagttttt atttttctac taaagcaaac attaattcac ctttacagac
    24481 aagttcttca cctacatatg ccttagcatc tgccttagct atattttttc taagagttgt
    24541 catttcaact tccatagtta atgtatctcc tggtcttact tctctcttga ttctaacttt
    24601 atctatacct gcaaatacac ctagttttcc tttgttttcc tctacagaca tcattgccac
    24661 tccacatact tgtgctagag cttctactat tagtactcct ggcatcaaag gatattctgg
    24721 aaagtgacct tggaaaaatg gttcattaac tgttatattc tttataccaa cagctctttt
    24781 cccaacttct agctctgtaa tcttgtctac taataaaaat ggatatctat gtggtattaa
    24841 tttttttatt tcatctatat ttaacattct aaactctcct aattattttc ttttgaaaga
    24901 cccaccagtt tcaagtaagt ctattgaacc taaagctatt cctgtacctt ttgcaacaca
    24961 agataatggt tcatctgcta ttgtaacagt tattcctgtc ctctgttcta ttattttatc
    25021 taatccatat aataatgaac ctcctccagt cattattata ccgacatctc ctatatctgc
    25081 tgctaactca ggaggcgttt tttccaaaac agcatgagta gcaactacta tttgctcaac
    25141 acattctcta agtgcttcca acatttctgt tgaatttata gttatagaac taggaagtcc
    25201 tgtaattaaa tttcttcctg ttatcttcat atatttttct tcttctctct taaatgcaga
    25261 acctatttca aacttgattt tctctgctgt tctttctcct ataagaagat tgtgttgttt
    25321 tttcatatat ttaactatag catcatcaaa tctatctcca ccaaccttta tagattcact
    25381 tacaacagct cctcctagag atattacagc tatatctaca gtacctccac caatatctat
    25441 aatcatatta ccattaggct gtgatatgtc tacaccagct cctatggcag ctgctattgg
    25501 ttcttctatg atatatactt ctctagctcc agcttgtctc gtagcatctt ctactgctct
    25561 tttttccact tctgtgacac ctgttggtac gcataccata attcttggca taaaaaatct
    25621 tccaaatccc tttttatcaa ctatcttatc tataaaagat tttaacatct tctcagttac
    25681 atcataatct gaaattacac catctcttaa aggtcttata gcgactatat tacctggtgt
    25741 tctccctatc atctttttag cttcttctcc cacagctaaa acagaatctg ttcttttgtc
    25801 tatcgccaca acagatggtt cttctaaaac gattccctta ccactaacat aaacaagcac
    25861 atttgcagta cctaaatcta tgcctatatc agctccagcc attttttcaa ctcctttatt
    25921 tataatatta gattatatta taagcctatt ttaatactcc tataataaag catatacaaa
    25981 aatctcgctt taatcaagag aaaataaatc ttttgagagt attattattt tgctatttat
    26041 aattacttaa tatatttctt tttataattt aattcaataa aaaaagtagg tttttaacct
    26101 acattttttt atgttccttc tcatatttta atttcgttgc taatccccct ctaatatgtc
    26161 tctctgattt atttttgtcc agcacatttt taacttcttt ggcaagggat ggatttattt
    26221 ccttcaatct ttctgtaaca tccttatgaa ttgtactttt acttactcca aatgtcttgg
    26281 cggtttgtct tactgtagtg tttttttcaa gtatatattt tgctaccact atggcccttt
    26341 cctctatatg agatctcaaa gattaccccc ctcacataat agctttaatg atatatatgt
    26401 aaaaacacca aaaaatataa caagcttaac caaatatgat taaacttgtt atactattac
    26461 ttaattagac tcattgggtc tatagatttt tctccactat acgcctctaa atgcacatgt
    26521 ataccttctt cactttctat ttgagaagta ctacctacag tacctagaga ttgtccttct
    26581 gttaccttct gttctttctt aacactaaca tttttatcta gatttgaata tactactaca
    26641 atgttattgt cactttttat tttgactgat tgtccatatt cttcatcatc aaatacatct
    26701 actactattc cgtttaatag agattttact tctttgcctt tagtacaact aatatctaca
    26761 cctttatgag tttcccaaac atcaagtgtt ttagaataac taggctcttt ttctgaatat
    26821 cctcttatta ctttatctcc aatgtaattt aattttgttg tactcgattt attttcctct
    26881 tttgacttag ctttttctaa gttttgtttt gaatccgttg cagtagggat agcatctttc
    26941 ttgtcttttt caattaaatg tatttcctct tcactatctt tattagcatt ttccataatt
    27001 cctttattag aagccaattt atctacatta ttatttgtaa accaaactcc acctactgct
    27061 aataaacaaa cacatacaaa caaagataaa taaaaaccat ctttttctaa cagctttttc
    27121 ttcattttgc acctccaaat atatttctac atttggatta ttgacgtgtt tttggcattt
    27181 tatacaatta ttagtatata tcttttattt ttgtatctgt gtagtaatgt gataatatat
    27241 cataatattt ataaccctct tctgccattc cttctgctcc ccactggctc ataccaacac
    27301 catgaccata tccttttacc acgaaatcta tgtatccttc tccaaatttt atgtcaaagt
    27361 ttgcagagtt caacttaaaa acagttctaa tgtcttttcc agtcaattct ttatttccta
    27421 attttatttt ttctacagtt ccggcatcac ttcttttagt tattgaaact tgtttactta
    27481 aattattcac atctatcaca atagtgctat atgcccttct aagactttta acaaaatctg
    27541 tgttacttat tttaagtgtt gacgcaaatt taggggaata cttatcataa ggactttcca
    27601 cagactttaa ataaggatat tttgcagaaa atacttcttc actattttct gtttttcctg
    27661 aagatgtaga aaaataaaga ggaagtatcg ccttatcatt ataagttatt atttgacctt
    27721 tagttcctct aactgcttct tggattttgg aatatttatt cttaatccat tcttcaccat
    27781 ttaatttttt aagagtatca taactcttat attcttgaca atgtttataa tcagtacata
    27841 ctactgcatt tttatgttta ctagatttac catgttcctg tttatatact acatatgttc
    27901 tagctgctac agattgagct tttaaagctt ctatatcaaa ctctgaagac atctcaccag
    27961 ctagtactcc acataaatag ttctccatgt ccatcttttc ggttttccct gttatatgat
    28021 tatatacatt gataattggt gcttttttat ttacagtttc atagttcact gtattttttt
    28081 cttctttatt acctttgtct tcaatatcac tttttttaat agttttgttt atagatactg
    28141 ggctctctgg tttttcagtt aattctacat ttttataaga aactaaagtt ataagtgatg
    28201 gaactaacac agagcaagtc acaaatccta acaaaacaac caatgggttc ttcatatact
    28261 ctcctcctta gcttttaaaa ccttgtattt aagtatatga acaaccctat taatttataa
    28321 ctttcttcta taaaccaatt attaatcttc tactatttcg atttgaccac ctagtgctgt
    28381 tatcttctta tctatatcaa cataacctct ttggatatgg tatatttctc ctatttgagt
    28441 ttctccttct gctataagac cgcataaaat aagggctgcc cctgctctta aatcagtagc
    28501 gtttactgct gcaccgtgta gctcatctac accattaaca accgcacttc ttccatctat
    28561 ttttatatta gcacccattc tattaaattc agcaacatgc ataaatctgt tttcaaaaac
    28621 agtttctata actacaccag ttccattggc tacagtaagc atagccataa attgtgcttg
    28681 aacatctgta ggaaatcctg gatgtggtaa agtttttata tctattggtt ttaatacttt
    28741 tggacctaca actcttacag aattgtccat ttctgttatt tcacaaccag cttctcttaa
    28801 tttagctaca actggtttta aatgctccat taaaacattt tcaacagtta tatcaccttt
    28861 tgtcatagct gctgcaacca tataagttgc tgcttctatt ctgtctggaa ttacattatg
    28921 ttctgctcct tttaactctt taacaccttt tatttttata gtattagtac ctgcaccttt
    28981 aacatctgct cccatttcgt tcaagaagtt tgctaaatct actatttctg gctcttctgc
    29041 tgcattctct attattgtag tcccttctgc taaagaagct gccatcatta tgttttcagt
    29101 tgctccaaca gatgggaaat ctaaatataa tttatttcct actaactttt ctgttgctgc
    29161 ttcaacaaaa ccatggtcca tttctatctt tgcacctaaa gctttaaatc cttttaaatg
    29221 taaatctata ggtcttgttc ctatagcaca tcctcctggc atagaaattt tcgtactatt
    29281 aaatctagca agcaatggtc ccataaccaa aaaagaagct ctcatctttc ttacaagctc
    29341 atatggagct tcacaagttt ttatattgct tgcatcaaca gtaagtgtat tttctttata
    29401 ttcaacttct gctcctacat gtcttaataa atctgatata acatgaacat ctcttaaatt
    29461 tggtactcca tttaatgttg attttccgtt tgctaataaa gtcgctgcta ttattggtaa
    29521 aactgcattt tttgcaccat ctattttaac actaccttta agtggattac tcttttttac
    29581 tattatctta gccatatttt ttgatctcct cataattaat aatctaattt tataattggt
    29641 gtggcaatat atatgagtgt ttttccgtca tcctcattat atttaattcc tatatttaaa
    29701 ttaatttttt cccctaaata ttctaattca taatcattta atactttact atatgctgtt
    29761 atagataaaa agtttacgtc tttaacttca tctatttgct tagcactcat attatataat
    29821 attttttgta aaatatcatc atatttgttg ttttgtaacc tttttgtgta ttctccaaca
    29881 atacaagcag acacatctac ttgagatgaa tatttattta acacattttc tacgttacta
    29941 taaataacct ctttatgttt atatactttg ttatccaata tgtcaactac tatgtaactt
    30001 tctttgttat ttttatttat tccttttata gaaatttctt tatcgctacc ttttatagta
    30061 gcacataatt gtattttatc gccagaccct ttttgattcc aatttattcc ttttttatcc
    30121 aactttagat tgttaattat ttctataaaa atattatgaa attctttatt tgataaatta
    30181 tgatttataa cagaattaat tttaacatta taaaatttaa aatttgcttc tgatatttca
    30241 agagctttac ttaatctatt gtatctagta tttatacttg tatctgcata agatattgtc
    30301 attcctataa tcattaatat aaaaaagctt atagctaatt ttactttttt catagtaaaa
    30361 aatccccctt aaatatgttt ttctgtataa tagaattatt aacaaattta agagagatat
    30421 actttctcaa tatgtatttt tatacatttt tgtatttttc atctaataat ctctttcaaa
    30481 ctcttcaatc tcaattttct ctatccttct cctggacttg ccaatttctt atatgtatca
    30541 aatacatctt gcattaattc ttatatattc tcttctgtaa tataaattac ttagtctata
    30601 cttaaatcta taatctataa tctataatct ataatctata atctataatc tattcatatt
    30661 ttaccaacaa aataaacaga taatactttt ttatcaattt ccatagctcc aaatgaacat
    30721 acctaagtac acttcccaat acacaattta cacactcttc ctaataaatc aaaaacacct
    30781 ttgttttaaa caactctatt gcactttcaa aaactttatt tgtgttctcc cacttcaatt
    30841 aaatttgaat tatcaagact ccagcccatc catccatcac tataaaggga ggtattttcc
    30901 actcctatga catttgcata tgttaatact tcagcagcac gccatccact tccacacata
    30961 aatataagct gtttattcac atctatattt tccttgtccc acatttctaa aatctcatat
    31021 ttatttctca ttgtattatc aatattacga tattcttcta gtgaaacact atcactacct
    31081 gaatgtccat ataaagctcc tggtatacgc ccttttttat cataatatgt atatccagat
    31141 acttttccta tatgttcatc ccaagtacgg ttatctacta gtataaactt atttttttct
    31201 tttagttttt gtcttaattc agatgtagta acaattaatt gtgagttaac tggtatatct
    31261 gctccaaaat tagtacatga atgttttgga ttactaataa attctaattc atatcctgct
    31321 gataaccatg agtttgttcc accattcaaa actcttacat cttttactcc tatatatctc
    31381 aatataacag ctagtctata agatgccata ggtgtactac ttgaaactat tacagtatca
    31441 tcctttgtta atccatagtc taaagcaaac ttagttaagt tatcatcatt atcaagcatc
    31501 catgttggag gaggttctat aatatcagta tttacatgaa cacttgtagg tatatgccct
    31561 tttgtatacg attcttctct ccaactcgct tcaatcattt ttatattctt tgaatcttca
    31621 aatgtctcag gaatttttcc atctaatata tcttttatta taaaagcagg aactatcatt
    31681 tgataatttt tatatctttc cattggaaga ttttcgtcgt ctgcccattg tttaatatca
    31741 tatttatata gatatttata tcctttttta gataaataat ctgctacaac caaagcatct
    31801 tttccatttg catcatacaa tactatattt ttatctaaat caattctttt agttttcaat
    31861 gcttgttcta acacttcatc ttttctatct gagtatacac ttaaccaatt agcagaaaaa
    31921 tctactgctc cttttatatg tccacctctt tttactccat ctaacttcca gccattataa
    31981 gcatcattta agcgagtatc aactataacc caagcgttat cttgcacttt ttcttgtaaa
    32041 acttcagtag atattgcctt aagattaaca aaataatctt ctttcttttc ttttttaaaa
    32101 tcatttgtca taatttatac tcccaattta aaaaaatttt tatttgaaat tattttacta
    32161 aaataaagta attttcttat actctattag cttcattata ttcttttata aatagttcct
    32221 tatattctta ttttaaacta ctcactttat cttatcacaa ggttactaat accttgtatt
    32281 ttaatttttc tcataagcta ttattttttt atctgatatt gactatatgt ttattaagta
    32341 ctcactatat tttatatgaa ctatatttat cgttccctat acctggtatg gtctatatat
    32401 ttatcatttt acagatgatt taatatcaat ctttttataa aatttattat agcaaaaaca
    32461 aaacaaattt aatatttatc taaaaaactc atcctataaa atacaaataa caggatgagt
    32521 tcttttcaat aaatattatc tatttaattt ttaatctgtt tttattcaac tgttactgat
    32581 ttagctagat ttcttggctt atctacatcg catcctctta gtacagagac atgataagca
    32641 agtaattgca ttggtacaac tgctaatatt ggtgataaaa tatcatctga atttggtata
    32701 tagattattt tatcagcagc tttctcaaca tccttattat ggctttgagc tattgctact
    32761 acataagctc ctcttgctct tacttcttcc atattagaaa ccattttttc aaataacttt
    32821 tcttgagtag ctatggcaat tacaggagtt cctttttcta ttaaagctat tgttccatgc
    32881 tttagctctc ctgctgcaaa agcttctgca tgtatatatg aaatctcttt taatttcaat
    32941 gaaccttcca ttgctaagct atagtctata cctcttccta aatagaatgc atgttcagaa
    33001 ctaacaactg ttttagcaac ttcttttata tattcttcat tgtctagtat ttcttgtatt
    33061 tttgatggta tttctttcat cttagaaatc atactatcat aaaattctct agttattgtt
    33121 ccttttttga tagcaaagtc taaagctatc atgtataaag aagttatttg agtagtatat
    33181 gccttagttg atgcaactgc tacttcaggt cctgcccaag tatagaatac atcatctgat
    33241 tctcttgcta tagatgaacc tacaacatta gttattgaaa gtattcttgc tcctttagcc
    33301 tttgaatctc ttaatactgc tagagtatct gctgtctcac cagattgact aactaatatt
    33361 actaatgaat tttcatctac aaatgggtcg ctatatctaa attctgatgc tatatcagtt
    33421 attactggta tatttacaaa tttttctatg gcatacttac caagtaatcc agcattatat
    33481 gctgttccac aagccactat ataaacttta tttatttttt ctaagtcttc tttagaaata
    33541 tttatactgt ctagaattat atttccatta tcatcaagtc ttctttctaa agtttctctt
    33601 actcctgttg gttgctcatg aatttctttt gacataaagt agtcatatcc acctttagac
    33661 gcagcttcta catcccaagt tacatgaaat acttcttttt caacaagatt tctattagag
    33721 tcataaactg ttacattttc gtcttttaga tgaactactt ctccattttc taagaaataa
    33781 acatctctag tatattttaa taatgcaggt atatctgatg ctataaagtt ttcaccttca
    33841 ccaacaccaa ctacaagagg actatctttt cttactgcaa ctaattcatt tccatgttct
    33901 ttacatataa cacctaatgc ataagctcct cttaattttg atatagtttt gtatacagca
    33961 tctaataaat taccctcata gtatttatct actaaatgtg ctattacctc tgtatctgtt
    34021 tgagattcaa acttaacacc ttcagaaatt aattcttctt ttatttccat atagttttct
    34081 attatcccat tatgaacaac tgctattgtt ttggcttgat taaaatgagg atgtgagttt
    34141 acatcagatg gttctccatg agttgcccat ctcgtatgcc ctatacctaa atttccatca
    34201 ataggattct tttctaagtc ttctgcaagt acagaaagtc tacctttaaa ctttctaata
    34261 ttaagttctt tttcattaga actattaact gctactcctg ctgagtcata acctctgtac
    34321 tctagcttag aaagcccttc tactataact tctgctgcct ttctacttcc taaatatcca
    34381 actattccac acattatttg tgtcctccta aaaatattat ttatatattt taagagttag
    34441 gtcatataag tttactttgg cttccctttg tccataggat tgctcctatt atttaaagaa
    34501 gtctttcgat ggtaaacaca ccgcagagca tccgccgaca attcgattaa ctctgacctc
    34561 gtcaacttag ttcctatatt actcaataat tatgtctata actaagttct ggcgcttaga
    34621 aaggtgtcct cagtatttat atctgagaat accctttaaa ttacgataat ttttcttgta
    34681 taagattagc taagtttgtt gctaattctt ttatttgacc ttcctcttta ccctctaaca
    34741 taactctaac taatggctca gttcctgatg gtcttatcaa aactctacca tttccgtcta
    34801 aaatagattc tattctttct atctcagttt ttatttctgg atattccata tatttgttct
    34861 tattttcatt ttttattgtt gcatttacta atacttgtgg gtattggctc attattgatg
    34921 caatttctga taaaggtttc ttttcttgca agattatatt tgcaagtatt agagaactaa
    34981 gcactccatc tccagtagtg ttgtaatcta aaaatatcat atgtccagat tgctctccac
    35041 caagattata tccattttta accatatctt ctaaaacata tctgtctcca actgctgtag
    35101 tagataaatt tattccattt tccttagcag ctattgtcaa ccctatatta ctcataactg
    35161 taactaccaa agtatcttgt gccaatttat tatttttctt taaatttaag gcactcaata
    35221 tcattatatg gtctccatca acaatttgac ccttttcatt tactgcaatt aatctatcag
    35281 catctccatc atatgcaaga cctaagtcag cattatgttc caaaacagcc ctttgaagtt
    35341 gttctggatg tgtagagcca catttatagt ttatattatt tccatctggt gaactattta
    35401 ttgaaattac acttgctcct agttcatcaa acactattgg tgccacttta tatgacgcac
    35461 cattagcaca gtccaaaact acttttaatc ccttaaagtc agtgcttatt attgatttta
    35521 ggtagtctac atagtctctt tgtgcacaat gttcatgtaa ttttcttcct acattttcac
    35581 caattggaag acaatcaatt ttgtctatat cgtctatata ttcttctatt ttcaattcaa
    35641 tttcatcatc taatttatat ccattcttat taaagaattt gattccatta tattcaacag
    35701 gattgtgaga tgcagatata accacaccac aatcagcacc atattttcta gttaaatatg
    35761 ccactgcagg tgttggtatt attccaacag taataacatc acatccaaca gacataagtc
    35821 ctgctattaa agatgcttcc aacatatcac ctgatattct agtatcctta cctactacta
    35881 cttttactct gtggtctcct tgagctaaga caaatccacc agctctacca agtttatatg
    35941 ctaaatcaca tgttagttct gtatttgcta ctcctctaac tccatctgtt ccaaaatact
    36001 ttctcactaa agattctcct ttcataattg aaaagcttgt aatttgtatt atatttttat
    36061 agttcataaa atatcattat aggcttaatt tcagtcataa aaatatttta atacttatca
    36121 gcaaaaaaga caatagaaat aattctattg tctttttttt gacatctttt tctttattag
    36181 ttagttgctt tagaacatgt agcatccata cctttttgta aagcttctct gtttaatgga
    36241 acgaattttt cttttctagg tccgaaaact tttttgaatg cttctagtac agattctata
    36301 tctacagtat cagatacttt taagtaagcg cctaacatta tcatgttagc aactttatca
    36361 tttcctaatt cagctgctaa ttcatttgct ggtatataat aaacatctac atctgttcta
    36421 tcaacttttt cttttattaa agaactattt actattattt ttccaccagg aataacatca
    36481 tctttgaatt tttcaaatgc tggtatattt aataatatag ctgcagttgc atcatctgtt
    36541 ataagtggag aaccaactgg ctcattagat acagttactg cacagtttgc tgtacctcca
    36601 cgcatttctg gtccataaga tggtaaccaa gaaacttctt ttccttctag cattcctgca
    36661 taagtaagta attgtcccat agacataaca ccttggcctc cgaagcctgc acatattact
    36721 ctagctgtag acatttactt cacctcctca acttcaacat ttttgaaatt tcctaatgga
    36781 tagtatggta tcatgttgtc tcttaaccat tgtaaagcat cgtttggagc tagtccccag
    36841 tttgtaggac aagtagataa tacttctact attccaaatc ctaatcctgc ttgttgaact
    36901 tggaatgctt tctttatagc ttttttagct tgtctaacat gagctggagt atcaactgct
    36961 actctttcta caaatacagc tcctgttaat gtagctaaca tttcacttac tcttattgga
    37021 taaccttgag tttcagcatt tcttcctgat tgagcagtag tagctctttg tccaactaat
    37081 gtagttggag ccatttgtcc accagtcata ccataagttg tattgtttac aaatatagta
    37141 gttatttttt ctcctctagc agctgcatga actatttcag cagtaccaat tgaagctaag
    37201 tctccatcac cttgataagt gaatactgta ttttcaggat gaactctttt tattcctgta
    37261 gcagcagctg gcgctctacc atgagcagct tcttgagtgt cacagttaaa atatttatat
    37321 cctaaaactg aacatccaac tggaacaaca cctacagcat cacctaatac tcctagttct
    37381 tctaatactt caccaactaa tctatggatt ataccatgtg tacatcctgg acagtagtga
    37441 gtttgagtat cttgtaatcc ttcagttttc ttaaatacaa cagccattat ctagcacctc
    37501 ctgcaaccaa ttctccggca ataatttttt tagctttttc aactatttct gctggtgttg
    37561 gagtcattcc tcctggtctt ccatggaagt aaactggtaa tttaccttct actgccattt
    37621 taacatcttc caccatttgt cccatactca tttcaacagt caatatgttt ctagcattag
    37681 gaatttggtt gaatgcttcg aatgggaatg gccataatac tttaggtctt ataagacctg
    37741 cttttattcc ttcttctctt aatatatcaa ttgcgctttt tactactctt gaagtagttc
    37801 cataagctgc aaatacaaat tcagcatctt ctgttttgta catttcgtat tgtacttcat
    37861 tcttttccat tgcatcaaat ttttcttgta aatgccaaca atggtcttct agtacttctg
    37921 gctctaagta tagagagttt attacattag gttttctttt tcctttagtt ccaacagttg
    37981 cccaatcttt aggaggcaat tctcttttct tctcaggagc tctaaattca actggctcca
    38041 tcatttgacc tatcatacca tctgcaacta ccataactgg tgatctgtaa taatctgcaa
    38101 cgtcaaaagc ttccattatc atatcaacag cttcttgaac tgtagctggt gcgaatactg
    38161 gagttctgta gtctccattt cctccacctc ttgtagacat aaagtagtca gcttgtgaag
    38221 gttgtatact tccaagtcct ggaccacctc tcattacatt aagtacaaca caaggaactt
    38281 ctgctcctac tgcatatgtt ataccttctt gctttaaagc aactcctggt gaagatgaag
    38341 aagtcataac tcttgctcct gcacctgctc caccataaac catgtttatc gcagaaactt
    38401 ctgattcagc ttgaacgaac gctccaccta ttttaggtaa ctctcttgat aaatattctg
    38461 gtaattcact ttgtggagtt attgggtaac cgaagaaata tttgcaacca gcttctatag
    38521 ctgcttttcc aaatgcttcg ttacctttca taagtatctt agccattaaa attccccctt
    38581 aaatattaat taaattaatc tctttctact gttataacta catctggaca catagttgca
    38641 caacttgcac atcctatgca tttttccatt tcaaaaacag ttgctgggtt ataccctttt
    38701 ttgtttatta catttgagtc taattgtatg atttttactg gacatgcttc aacacacaat
    38761 ccacaacctt tacaacgttc ttgattaaaa gatacttttc ctttagccat ttacctaagc
    38821 ctccttaaac tttctatata tttgagctca aactacatcc agtcttctct catatataat
    38881 tttataggaa aaatttctcc ttctaaatct tttggtaact gttcaacaag attttctaaa
    38941 catgcaacat atcttatcgg tatattacat ttttcagaaa cttcttttgc aactttctga
    39001 cctcttaaaa catcatcaat agtagtaaac ctaataagat gagtattatt aattagacct
    39061 gttaccttta atttagaaga tttttctatt tcttttatat attggataac ttcttcagaa
    39121 gtttgagttt tctccctatt tgcatttatt acaaaaagca tatcataatc gccttctttt
    39181 aacaaatgac taaatcttcc tataacccta gcacctacat tatctccacc taaatctata
    39241 acagaattgt atgaatggtc taccatagga gacattacct ctgctgatac tgctggtaaa
    39301 tctaatgttg gagcattaat tgagctatct attggttgta tacctaagga tttcattaac
    39361 tctttctttt ctctagttct aaagtataca tttaccacat ctaaatcggc tatagcaact
    39421 ttgccagaaa ccatatctct taacttaaca acatagttca tcgcaaattc acttttacca
    39481 cttccatagt gaccagttat gattcttatt cttttatcat tagttatcat aaacttgagc
    39541 ctcttcttca ccagttaaaa ctctaagtcc accttgagct aatgcaatca tttcatcttc
    39601 acctggataa acttttacat ctgctataaa tttaactcta tctgcaatca tttctgtaaa
    39661 catttttgaa tatgcgattc caccagttaa taatattgct tttacatctc ccttaagaac
    39721 tgcagcacta gctcctattt ctttagagat ttgatatgcc atagcttcat atgctaattt
    39781 agctttttca tcaccagctt caattctttc ttcaacttct ctagcatcat tagtgtttaa
    39841 gtatgcaact aatccgccat taccttttat tttcttttta atttcatctt gagtatattt
    39901 tccactaaag cacattttta ctaatgcacc tactggtagt ccaccacttc tttctggaga
    39961 gaaaggtcct tctccatcta atgcgtttgc aacatctact attttaccat ttttatgagc
    40021 tccaacagaa actcctccac ccatatgtgc aactataaga tttatatctt catatttctt
    40081 gtttatttct ctagcatatc ttcttgctat tgccttttga tttaaagcat gtactacact
    40141 tgctctactt atttcaggca taccagaaat tctagcaaca tcttctaatt catctacaac
    40201 aacagggtct actatgtatg aaggaacatt tacttcttca cctatttgtt ttgctattat
    40261 tccacctagg tttgaagcgt gttctcctaa aactcccact tttaaatctt caatcatagc
    40321 agcacttact gaataagtac caccttttat aggtttaaga agtcctcctc tacctactac
    40381 agcatctaat tcagatgttt ttactccacc ttcttttaga gcttcttcta ttacttgttt
    40441 acgaaattca aattggtcag acaccttctc atattttcct atttcttctg aagaatgtct
    40501 taaagttttt tcaaatacta aatcctcatt atcaaataca gctattttag ttgatgtcga
    40561 accaggattt attgttaaga ttttaaatat tttgctcatt tgcctatttc ccccttaaaa
    40621 ttatgccttt gctgccatta aaacacctaa agctattgag tttagtttag tttcttcact
    40681 gtctgctcta gaagttaata ttattggtgc tttagcccca actataactc ctgcattttt
    40741 tgattttgag aagaatacca aagctttata taatatgtta ccaccttcaa tatctggggc
    40801 taataatata tcagctcttc ctgctacagg atgatttata cctttatgtt tagctgcttc
    40861 taaagatact gcattatcaa ttgcaaaagg tccaccaacc atacaacctt tgatttctcc
    40921 tctttcatac atttcttcta gttctttagc ttcaactgta tctttcattt ttggatttac
    40981 tttttctttt gcgcatattg cagcaacttt tggttcactt atatctaatg aatgtgctac
    41041 tgtgcaagca ttttctatga tttgcttttt agtatttgta tcaggagcta agttcatagc
    41101 tgcgtcagtt acgaaaaata atctatcata tccctctaca tcaaatactg ctacgtgact
    41161 taatacattt ccagttctaa gacctacttc tttatttaaa actgctttta gtattattga
    41221 tgtgtctact aagcctttca ttaccatgtc ggcttttcct tgtgaaacta attcaacaga
    41281 ttttagagat gcttctgcta aatcttttat atctatcagt tcataatttt cgatatccat
    41341 gtctatgctt tttgcaattt ctttagtctt ttctatatct cctactaaaa tggcatttgc
    41401 tattttttct tttctagcca tttcaactgc cattaaaact tctttatctt ggcaacatgc
    41461 tactgatata gttttaggtc ctctttcttt tgcaaactta attacttctt caaaacttct
    41521 catgcatttc cacctcatct tgaatttttt gaaatagaac tttttatatc tcaattaaat
    41581 tataactcgt tcagcaacgt tttcctagag ataattgaaa tatttaattc aaaatattta
    41641 tttaaatcta tttatcagtc ttttttcata tatctagcgc tttttagatt taacaaatat
    41701 ctcattgtcc ttattttata atataactaa aataaacaag atgcaacaaa aacataaaat
    41761 taacaaactt aattttatag gtttttttgt cgcatcttgt aacaactaaa tattcgaaat
    41821 atttgttcat tccacttcaa caatattagg ttctatattt atttttttaa attgatatgt
    41881 tgtttcatat tttattttat atttactatc ttcctcagga ccttgtgata aatcaatata
    41941 taatgaaata tcttttttat ttaacttact caaatcctcc attgactcta caacaacttt
    42001 tatatctaca tcagaaatta catttggatt atccatattt gttgggatat ttcttaattc
    42061 aatatctccc gcattatata aaaattctgc actcaattgt tttgtatcat ttaatttaat
    42121 tgttatagag ctatcctcaa gagttatacc ctctggaatt tctaagctaa cttctttttc
    42181 ttcaccatta gccaaatcag ctaagtcaat aggttttgtc ttaatataac ttattttgtc
    42241 tatatcttct ttctttcctt ttattgtaac ttgttcctta cttaatttat agtttttcag
    42301 tacatcatta tctttattat catttagact tactcttatt gggacaattt tttccttcaa
    42361 tagttctatc tttgcagtca caaaggatga acttaatgtt actccttcaa ccttagttcc
    42421 atcactattt aatggtatta attgcaattt tttagaaaaa tcatctgtct cattaccaac
    42481 atctagatta gctcttacac ttttgacttc tttaactaga gttcttggac ctgatacatc
    42541 gacaaattcc ttactaagtt caattttatt tatatttctt tttgaatttc cacttacttc
    42601 tacctttata tctctttttt cttttatgtt tttttcaaga tttactacta aaacatcatg
    42661 cttaaattct agagtaacac cttgaggaat atttgctttt aaatatactt ccttctgtcc
    42721 ttgtattggg tcttcaatgg ttccatatac atgtatatct tcttttttaa cactttgtac
    42781 ctttgataaa ctacctttta tatatacatc tgctgttaat ttttcgtctg gatatataac
    42841 aagattttta tcctttaaat cactcatatt acttatagat acaggtatat cttcaaaccc
    42901 tttagtttct tcagggtcaa ctacagccat aacatacatc cataatgcaa ttgcacttaa
    42961 aaatgatatt atttttattt tggtattatt ttttagtcta tttatcattt aaatatacca
    43021 cctttgaaaa aacttcttgt ttctgtattt gtttttaaat tactacgtaa tatattcatc
    43081 attctttctc tagaaatatc tctatataac ttacctgctt tagcaattga tacgccacct
    43141 gtttcctctg atacaatcaa agttatacag tcagacacct cacttacccc tattcctgct
    43201 ctatgtctag tacccaaatc tttacttaaa tccttacttt ctgtcaatgg caaaaaacat
    43261 gcagctgctt ttaccttaga atctctaatt acaacagctc catcgtgtaa tggtgtattt
    43321 ggtataaata tatttattaa tagttgccta gaaatttcag catcaataat agttcctgta
    43381 tttattatat cacttattct agtttctctc tccattatga taagagctcc tattttttgt
    43441 ctagaaagtg aatataaagc ttctacaatt tcttctatat ttctattgag agtttcttca
    43501 gaagtattta cattctttcc aaataaatta aactttgctc ttcctatatg ttcaagtcct
    43561 gctctaaatt ctggttgaaa aattataaga gcagctataa ctccataatc taaagcttta
    43621 agagatatcc aataaacggt atgcaattta aaggtgttac taagttgagt tgctaataaa
    43681 agaaatatta tacctttaaa cacttgttct gctcttgtat cttttataaa cataaatatt
    43741 ttgtaaaaaa tatacgctac tatagatatg tcaattaaat catatatact cattcttaat
    43801 attatattaa aaaatccatt taaaaaagaa tttatatcta acacagtttt atcctcctgt
    43861 ttgataactt cttaattatt tacactaagt attatactat ataaatctaa ctctatacaa
    43921 actatttaac ttttatgtaa ataattctag ttttatattt ggagattgtt aaataatctc
    43981 taaacacatt aaaaatatca acttaataaa tacatatttc ttattaaagt tagtttttac
    44041 tcaaataact caaaccatca aggcgcgaat acaaaatagt actcgcgcct taattcagtt
    44101 ttatgttcta aaatatctta atatatcttt gtttaatata tattaattat tataaattct
    44161 ataatccact tattaactat aaacaaatac aaaatctaaa tattacttat catcaatatt
    44221 attccattag attcattttt atattaaagt aataacttat ttcatatcta gattagataa
    44281 atttatttta atatcttact ataaactatc attaaaactt attatgtata actagatata
    44341 atctcctaca gtgaaaataa tacacaatac ctaa
//
LOCUS       DAMJYR010000022        44045 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.022, whole genome shotgun sequence.
ACCESSION   DAMJYR010000022 DAMJYR010000000
VERSION     DAMJYR010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 44045)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44045)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44045
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.022"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            110..460
                     /locus_tag="PC471_001141"
     CDS             110..460
                     /locus_tag="PC471_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HDF2788279.1"
                     /translation="MSAKVTNQYGNIEIDNQVIAQVVYRAAMESYGLVGLAYKSKGIV
                     ELLKGENATKGVRVEEVEEDAIAIELYVIIQYGTNISTVANNIIDRVKYVVEKMTGVR
                     VTRIDINVQGIRVK"
     gene            479..2122
                     /locus_tag="PC471_001142"
     CDS             479..2122
                     /locus_tag="PC471_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HDF2788280.1"
                     /translation="MIQYIDGKRLREMFISGANNLQNNKELVDKLNVFPVPDGDTGTN
                     MSLTISYALKELAKVENDNISDIGKALSKGSLMGARGNSGVILSQIIRGIAKSIEGKS
                     KLSTEDLAKAFKNGSDTAYKAVIKPIEGTILTVVRESGEFAIKTAKKEKDVVKFLSML
                     VKESNSSLERTPDLLKNLKEAGVVDSGGKGLVLIYEGMLASIKGDNIEIKNVDLDTNI
                     STSMDFAKSTTSTDDIKYCYCTEFILESSKVEDTKIRDIMMAYGDSLAVVGDDGVIKV
                     HVHTNDPGNVLQEALKYGQLLTIKIENMKLQHENTLLDVEEKKENDSEPLEEEKEFGF
                     IATSMGEGLANIFKDFGVDHIIEGGQTMNPSTEDFMNAIKHINAKNIFIFPNNSNIIM
                     AANQAKELSDKNIIVIPTKNTPQGFAALVTFNGELSEDENKEAMMNALNSVKSGQVTF
                     AVRDTVMNEIDVKEGNIIGIAEGNLLSAGDYVDEVTSNLIEKLVDEDTAIITLFFGED
                     VTESQANELRTSLEEKFEDVDVELYYGGQPLYYYLISVE"
     gene            2203..4260
                     /gene="recG"
                     /locus_tag="PC471_001143"
     CDS             2203..4260
                     /gene="recG"
                     /locus_tag="PC471_001143"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HDF2788281.1"
                     /translation="MDLYKDVQYVKGIGPKKADKLNKLGIFTLKDLLYYFPRQFEDRN
                     NLKKIAQLEDGEKVTIKAVISSINTFSPKEGMTLTKIDVKDETGSAKLVFFNKSYIKN
                     TFRPGDSILVFGKVKKKFNNLELTSCELEYLTNSPKNTCRFMPVYQLTYGVTNKEIMS
                     IIRTVLEDKELIIQEYMPQRIIEKYRLCSIDFAVRNIHSPSSKESLKIALYRIVFEEL
                     LILQLGLFVFKSGRNKEDGIKFETSKDLKKIISALPFKLTKAQNRALDEIIQDMNLEK
                     IMNRLVQGDVGSGKTVVALLALANCVLNGYQGALMAPTEILAGQHYISLTESLKDFGI
                     NVGLLIGSLTKKQKDTVLEQIKNNEIDILIGTHALIEDKVEFNNIGLVITDEQHRFGV
                     MQRSKLSLKGANPDILVMTATPIPRTLALILYGDLDISIIDELPPGRQPIETIAIEKS
                     KRDRAYNNLVRREVESGRQVYIVCPLVEESEAIEAKSAVELVEELRAEYFHDLRLGLL
                     HGKMKSSEKDEVMRLFKDKEIDILVSTTVIEVGVNVPNATLMIIENAERFGLAQLHQL
                     RGRVGRGSHKSYCVLIYDSKTDVCRQRMAIMEETNDGFKISEKDLEIRGPGEFFGTRQ
                     HGLPELKVANLFKHIKILKLAQQEARYILGEDNNLQLKENMALKKEIIDKFKDTLKEI
                     SLN"
     gene            4319..4873
                     /gene="rsmD"
                     /locus_tag="PC471_001144"
     CDS             4319..4873
                     /gene="rsmD"
                     /locus_tag="PC471_001144"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HDF2788282.1"
                     /translation="MRVISGKARGLKLNTPKNEDVRPTTDRVKESLFNMINSYIMESE
                     VLDLFAGTGSLGIECLSRGAKSCTFVDISKESIDIVKSNIKKARVESESIILNLDFKT
                     AIDKLKLQNSKFDIIFMDPPYYKNMFIEAIEKIDNSNLLNEDGIIVVEHDTNDLFPDK
                     ICKLEKTRDKKYGNTTLTFYKMEA"
     gene            4875..5372
                     /gene="coaD"
                     /locus_tag="PC471_001145"
     CDS             4875..5372
                     /gene="coaD"
                     /locus_tag="PC471_001145"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HDF2788283.1"
                     /translation="MENKPRKAIFAGSFDPITNGHLDIICRASKLFDELQIGVLNNPN
                     KKGLFSFDERVKLIEKSTSHLNNIKVVTFDGLLINYCQENGIGALVRGVRSGADVDYE
                     LQMAHMNRELNPDIETIILPSCTKYSFISSSLIKEVLLFDADIKNLVPKIVLEELKKK
                     TSGGN"
     gene            5376..5900
                     /locus_tag="PC471_001146"
     CDS             5376..5900
                     /locus_tag="PC471_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2788284.1"
                     /translation="MKIDLEMIELFEQLEEIFRSASTIPFSHKCTVDKDEVLAIINDI
                     RTLIPEEVTQAVWINKERNKIISQAKQDATNIVEQAKKEAERIQQEYQDNIEELKKNS
                     EDVVKAYVESSEPVVQADQRAKDIVDRAERIANEIRIGSIEYAEDVLSSVEYNLREIL
                     EEVKRDKAELRPKK"
     gene            6244..8163
                     /locus_tag="PC471_001147"
     CDS             6244..8163
                     /locus_tag="PC471_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HDF2788285.1"
                     /translation="MKERAKKILDFIIKNNNVTSQELQEKFNVSKRTIYYDILAINEQ
                     LGKSGNIKNVKHKFIFEGNLCDARKIISTEEDKFLDSDYRKTFILNKILLGEKISIEK
                     LTNEMLLSKNTVVQTITDVKKYLQTMGLRLEYKGKYKIIGDEYVIRELFLIIVQENIL
                     EINSISEEVSSFDTKGHIKLTDYSLLNLTKFVEFLNKRIRDGKTLYSYKYLNEAKKIS
                     YFSNCKELLCEEANENEQAYICTYISSLPSLNSEVKEDVVEEYVDKLIDKFEVNTAIK
                     LESKHEFKKNILRHLHSSYNRIRFKFPIRNPMLDETKYKHESLYKIIKSIIENEEEFP
                     VFEGIREEEIGFIAAYFGGYLRGSRDNGLRRNKVLLVCPNGLMVSKSLEIQLYKYIPT
                     IEIVGIVSIKQLKEVNVYYDYIITTIDIQNVNNVIVVNPLLTSSDVQLLMNKLISVKE
                     NEKYFNLELIIQAIRKNGVINNEEALKADLLNIIHKIDEGEMYQPMLKELINAERVNI
                     IKSVRDWKEAIKIASKPLLEDNSIEELYIENMIKSVEKYGPYIVLADRFALPHASSKE
                     GVNKLAMSLLIVEDEVDLLGKPVNIFMVLAAVDNTTHIRALASLSEMMYEEENVKLII
                     NGDKSSILELINKQN"
     gene            8176..8463
                     /locus_tag="PC471_001148"
     CDS             8176..8463
                     /locus_tag="PC471_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDF2788286.1"
                     /translation="MKILAVCGFGVGSSMVLKMTIQKALKQLGIECEVENTDINSARG
                     ADCDCIFTSQELLNELKGTCNVDVYPVKKYMDLAEVTEVVQTFLSDKNDKK"
     gene            8596..9855
                     /locus_tag="PC471_001149"
     CDS             8596..9855
                     /locus_tag="PC471_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HDF2788287.1"
                     /translation="MLDFISNNILRNPSMLIGLIAAFGLVLQKKKASDVIKGSLLAAF
                     GIIIMETGTGMLVSSIAPINGAFQSISGGAVTKGLSDVTFTASYGGIIGLAMFAGLAI
                     HLMIARFTPIKTIFLTGHMLYWFPFIFVASGVEAGLNGGVLIAFAAVLSALYWSIMPW
                     LLRKYVFDVIGDDSFTLGHPSGCLALISGFVAKLVGNKEKSTEDINIPQSLSFFREIS
                     ISGGMAVFLMFIVVGIVIPGAFKDSNEFIGTYAIKQGLSFGAGLIIMLHGVRMLINQI
                     MPAFQGISEKVVPNALPAFDCPILFNYKPNAVLMGFLVAMLTSTAMILICNYFNVFNL
                     LLIPLVITSFFECGAAAVIGESQGGIRGAIIGTAIAAIFMVVLVGISAVIYSGTIQNW
                     ILIFGGNDLSLFGIISRFIGSLFGGIL"
     gene            9858..10598
                     /locus_tag="PC471_001150"
     CDS             9858..10598
                     /locus_tag="PC471_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HDF2788288.1"
                     /translation="MKFKYIDEIRRLIDIIENEEKVNMEKAVQIFVEAIESKNAIFSF
                     GASHAGILTEELFYRAGGCVLINPVFAKSLMLDTAPITHTSKMERLEGYGTALADKTP
                     IKKGDVVIVHSVSGRNPVSIEFAMKAKENKAIVICITNLSYSKSVSSRHSSGKKLYEV
                     CDLVIDNHGQKGDACVEIDGLNQKVSPTSTVIATTVMNSIIAQATQELVNKGLKNPPI
                     FYSANIDGGDELNKKIFDEYKSVIHYEY"
     gene            10921..12231
                     /locus_tag="PC471_001151"
     CDS             10921..12231
                     /locus_tag="PC471_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HDF2788289.1"
                     /translation="MKKENKMEGIKVGMLKKLCSLAMVAIMTTTLITGCSSGNGKDSK
                     KDGEKEKLVLWMPPVENNMKEVWDPILDKFEEKNNCEVDLQIIPWDNYSEKFATAISA
                     GEGPDVGYMYAEMYPQFIESGAVEDLTNYATKEDKEQSIYIKTSEMMGGMYGMPFQAA
                     NPGVLYYNKDILEKLGEKPPKTWEDFKRICKKATQDTDGDGKIDQWGLAQGWGAKTFG
                     NLNWNWYPYLWQAGGDIFNDDLKSVKFNDKSGLEAAKFLKELQAYVPEDSLSKDSNEM
                     IESVFGPGKAAFTIMLSSAATSVFDESFPDLNWGFVTGLEDKKAATFGAVDHLSLISS
                     AKNKELAYKLIQHMLSVESMTEFHKAIPRAPITKGEPYQGDERFKEMVENDKNVYRPL
                     VVGPHGVEIYEYLWKELQTMISGDKTPKQALDDAAKYSNDLLAQ"
     gene            12329..13246
                     /locus_tag="PC471_001152"
     CDS             12329..13246
                     /locus_tag="PC471_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDF2788290.1"
                     /translation="MENLVREFEENSKRKKTKLNIKPYMYILPLGIILVAFYVIPIIM
                     SIYFSFTKYNIISPATFIGLENYKKLFTDETLKVSIINTIKFTVVVVPCQTILSLILA
                     VWITGKGNSKIASFAKGAIFIPVLSSMVLIGMVWRALLNGEGSIIYQVLGAFGIESSK
                     LLGDSKTALPTLMFISMWKNIGYFMVIYISAIMNLPKHCYEVAKVDGATKLQEFIKIT
                     VPLLKPTTIMVVFLGSIWSLQVFDLIYTVTGGGPGISTMSIVMHAFNLNFKNFNSGYA
                     MTVANVLFLLIAVVSILQNKLVKRDNSDF"
     gene            13262..14071
                     /locus_tag="PC471_001153"
     CDS             13262..14071
                     /locus_tag="PC471_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDF2788291.1"
                     /translation="MKTARRLLSDGLLLFIACASLVPFIYMLIISLKITYNSYSLDIS
                     FSTVTLQNYIDIFTKKGFAQYFFNTAIVSFSGVLLNLVFSTLAGYSFAKMDFKGSDKL
                     FLFMIMTLIIPSQVTMIPLYIIMKHLGWINSYLALIMPIPTAFGVFIMRQAILGVPKE
                     LLESAKIDGCSDFRILIQIVLPLIKPALITLAIFTFMGAWNEFMWPLIATTKDAMRTL
                     TVGLSTLKTFQITNYGQMMAGATITFLPPFIFYLILQSKFVEGVSLSGIKG"
     gene            14129..15877
                     /locus_tag="PC471_001154"
     CDS             14129..15877
                     /locus_tag="PC471_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4091 domain-containing protein"
                     /protein_id="HDF2788292.1"
                     /translation="MEISYVLKDCTFKHNKYSVVEAETWKDKDLDIVVAKGEKFAFQI
                     MLKATEEFNCTIDKSSAISWKGLGNRVRLALNMPNSLENNFSINILGYVQDDTKAFVN
                     DAILRDKDVLVEQYLPQTFWIEGQVPEDFEENNLDIGIDIFKSFGYEDEEKVCTIDVP
                     VKVRNVVLKPLDESKFFLDLWQHPSCLARMYKVELWSDIHFEIVDNYLKELASLGEKV
                     ATVIVSDYPWAGQSCYKVYKNPSNLYEYNMVSVSKGLDGKIKCNFESMDRYIGIADKY
                     KMAKEIDLFGLLGNWCAGEFGNPVEGYKDPIRVRYFDEKDKVFKFINNTNDLKEYIGL
                     VLNHLIECGLWDRVRIIADEPNNPEVVKECIEFINSTVGTHQVKYKSATHDQNFLDRA
                     KDEIDDMSINLKLTIQNYKDIESLKKKINDKGGILTWFVCCFPEKPNSFLSSPFVENR
                     IIGWYTYYFGLDGFLRWDYNLWTEDPWKDSSYKFPIWKAGDMFFVYPGKDLKPVRSVR
                     MENLRFGIQDFELFTMLEKEKVREYIEVELMQELLNKKENAEIKGFGDIELGYSLDNW
                     RYDKVKKYVLELLDRV"
     gene            16139..17161
                     /locus_tag="PC471_001155"
     CDS             16139..17161
                     /locus_tag="PC471_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 5 protein"
                     /protein_id="HDF2788293.1"
                     /translation="MFGGINIKRIGIIIMFFLSISFIGFYLVIRDNSDTVKMQRGINI
                     GNALESPKDFPWDVKMSNKFFDDIKDAGFDTVRIPVRFSDYTSDSDNFKIDEEFFKKI
                     DKYVDYALDKDLIVVLDLHHFEEIMKEPRVHKEKFLKIWQQIANRYQKYDKKLVFELL
                     NEPKENLSSQLLNEYIEEAIKIIRKTNPKRTIIVGPYNFYQIDYLNELNIPKDSNIVV
                     SFHYYEPNDFAFQGNIYHKGFEHLSNITWEGTNEQMDYLKKRFDTVENWANKNKVKIF
                     LGEFGITKEAPEVSRRAWIKAVREEAEKRNFSWAYWELASGFGIYNQIEGTWDRDILS
                     ALIEKR"
     gene            17166..19472
                     /locus_tag="PC471_001156"
     CDS             17166..19472
                     /locus_tag="PC471_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDF2788294.1"
                     /translation="MKKNSKKILITLTIITNVIYILWRIFYTVPKEEGKFALICAIIL
                     LFVEIMGMMEMFVHYYGMSNIEYPEKPIISEELYPHVDVFIATYNESVDLVRKTVNGC
                     IHMQYPDKKKVHIYICDDGNREEMRILAEKMGVNYITRTEREGAKAGNLNNAMQHTTS
                     PLIATFDADMIPMHDFLIATVPYFLKNEQAKKDGEKEEYEKVGFVQTPQSFYNPDLFQ
                     FNLHSEGRIPNEQDYFYRDIQLARNRTNSVIYGGSNTVISREALEEVDGFYTYSITED
                     FATGILIQSKGYRCYAIPEVHASGLSPTDLKSLIKQRERWARGCIQTGRRLNILFRRG
                     LGFWQKISYISSITYWYASIKRFVYIMAPILFSVFNVIVVKCTLLQVLVFWLPMYILS
                     SLSLKIFSQNIRNTRWTNIYETIMFQSLMPAVILETFAISKNKFSVTNKSKLEENRMY
                     KFLQGIPYFIYMVLSIIGILKMFVAIFKMSSMTYSVVLFWLIGNLFNLVMATLFISGR
                     QQLRKSERYIAEIDFKLKQNSYVLSSKTIDISENGFAFLLENPEYISPEEEFEVEFRE
                     KSGNEMYIANMKAKIVNVVEVNSKWKYAAYITHIEDSEIDNWMCIVHDRIPTLPMTIS
                     NQLGFFDDLQINVKKRIEKTRTLSRRSPRINMNFQMDIKNTGKLRIVNFNYQYVLLNF
                     ENKSIYPKEIALEINENIVLECDLCEGKIDERGILYKVNNIDSIMQNFFLRDEMMDWI
                     LQNKTILVSKPSEKKEKSIDEFEPMEYI"
     gene            19444..20559
                     /locus_tag="PC471_001157"
     CDS             19444..20559
                     /locus_tag="PC471_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2788295.1"
                     /translation="MSLNPWNIFRRDEMKTLKVFFCSTKYLILVFIFFINTTFISSAT
                     SDKILDLSFKKIETDLNSKITYEDTGVKIETDSSKSDKERYLYIYQNIKENWSMYNNF
                     YIEIQNKNKSSQRINLSIQSKNMLEFRLKEGSEVFLEGKNIIYSDKIKEGCIEVPGEF
                     EGKIYVNFNSLINEESNVVLDSNMLSNIVSWGITFIPSDEEHNIVIIKKISLLSEEKL
                     RFLNNIKIIGDEEVQIPVLGQSISQYEVLGLKSDSKIKYSLMGKQDNVSISQKGKLTL
                     SNKAKPGQIILQVNVDDKFKIGKKITLTESWSVNKKDKDGVPYTLVYPEQSPTVQDMK
                     KINFMNNIITFVRILFVSLVIICFGIYLYWKKCSKTK"
     gene            20577..21995
                     /locus_tag="PC471_001158"
     CDS             20577..21995
                     /locus_tag="PC471_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family protein"
                     /protein_id="HDF2788296.1"
                     /translation="MLFSSLIFLFYFLPITLVLYYVFRFNRTIQNMILLAVSLFFYAW
                     GEPKFVVIMIVSIIMNYIFGLLVDRYRESKIKVKVFLVLMCAYNIGVLFIFKYLAFAL
                     RNISELISTELTIPSIVLPIGISFFTFQGMSYVIDVYRRHGEVQKNPFYVGLYIAFFP
                     QLIAGPIVRYESVAEQILNRKETWNKFSIGTCRFITGLGKKVLISNNMAIVADYIYTM
                     NSQGEIAASLAWLGSIAYTLQIFFDFSAYSDMAIGLGLMFGFKFEENFNYPYISKSIS
                     EFWRRWHISLGMWFKSYIYFPLGGSRVANKDIMIRNMFIVWLFTGIWHGAEWTFVIWG
                     ILNFVFLIIERFIMFEKIENHNFIKHIYALLVVNFGWVLFRSPNLKEAYNYFKAMFGG
                     NGAIWSDYTYMFLKEYWVFFIFAFIFSVPIAKKINKFVVEQAKYSMIFNVFYPISAIL
                     LFFISVTYLVTGSYNPFIYFNF"
     gene            22010..23218
                     /locus_tag="PC471_001159"
     CDS             22010..23218
                     /locus_tag="PC471_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2788297.1"
                     /translation="MNRFFKVRLITAIMFLIILFAFSIFNVCYSGKDTIQEIKNSIAE
                     KSSLKDTISSIDNTVNDKVIYKNTFIETYGYLQKLMGKNEFSKFSVVKDTSGKMHYTY
                     FAKAPNPVGVIADRVRKFSDEMEKQNTKLVYLMTPDKYIKGVTKFPKGIPYSYSNETA
                     DNFLNQLKKYNVDYIDFRENILKSGIPKDDLFFKTDHHWKVETSFWAFGELVEQLNKK
                     YNMNLDENHYYRDKDNYNSIVYPKSFLGSMGRKTGILYGGIDDFTLIYPKFKTNYTYY
                     TDSKSQKFELKGRFEESLILSYPFNADLDLMDGQSDKYFTYLLGNRPLVKIKNIENPD
                     GLKVLFVKDSLIVPTAAFFSSVCSSIDMIDPRYYDGDILEYAKSHDYDFVFISVYPQN
                     LTKEFFPFCE"
     gene            complement(23292..24680)
                     /locus_tag="PC471_001160"
     CDS             complement(23292..24680)
                     /locus_tag="PC471_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HDF2788298.1"
                     /translation="MESSFLSQFLMITDFKTIFFIILLLGTFFIVRQFEKKKIKFSLR
                     TIYATVLGLILGIIIQAFAGFPNDTTQVIWLQEVSKWYSLFGSGFMDLLKMLVVPLVF
                     LSIIKVIINMKDNNLGTLTFKSLGMLLATTAIAAIVGIIVANIMKLGVGANMPTLVEN
                     QELKEINSLVDTLRGLLPSNPVMAMANGNIVATIIFATFIATSIKRLSKKHFETIKPF
                     IGFIEASYKIIVSVSMTVIKFMPYAVVALLANTITSQGISSIVSVLQFILALYISVAI
                     LFIIHLIFITLNGLNPITYIKNSTEALLLAFTSRSSLGTLPVTIESLVQNHGVDEGVS
                     SFTASLGANMGMNGCAGIYPALMAVTLANMAGVNMDMSFYVMLLIVITISSFGIAGLP
                     GTATMAVSVVISGVGLGSYFPLVGGILAIDPILDMGRTMINVNGAMVSSITVGKSLGK
                     IDKEVFSKKNIS"
     gene            25021..26355
                     /locus_tag="PC471_001161"
     CDS             25021..26355
                     /locus_tag="PC471_001161"
                     /EC_number="1.8.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-disulfide reductase"
                     /protein_id="HDF2788299.1"
                     /translation="MRVIIIGGVAAGMSAAAKLKRIKPEYEVVVYEKTEIVSFGACGL
                     PYFVGGFFNDADELLARTPEKLREAGIDLNIFREVVEVDSESKKIKVKNIKTDEIYED
                     YYDKLMIATGARSIMPPIKNIKLKNVSTLKSLYDGEYLKKLLSNEDNKRVTIIGAGFI
                     GLEAVEACKKLGKDVDVIQLEDRILPQVFDKEMTDVLEEEINRHNVNLHLDEMVVELC
                     GEDKVEKVITNKGEIDTDVVIIATGVRPNTEFLSNTNIKMLKNGAIVVDEYGRTSVED
                     IYSAGDCATIKNIVSNENVYVPLATGANKLGRIVGENLAGREISYQGSLSSSCIKIMD
                     MEAASTGITERQAKDLGINVKSKFISDYNQTHYYPGRNKIYVKLIYDADTKVILGGQV
                     AGFKDAVQRANVLAAAIFGKMTTNQLGMLDLCYAPPFARTWDVLNVAGNVSK"
     gene            complement(26497..27414)
                     /locus_tag="PC471_001162"
     CDS             complement(26497..27414)
                     /locus_tag="PC471_001162"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011969064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HDF2788300.1"
                     /translation="MIDLTLLGCGGNVPMPNRFLSSVFINYKGRKILIDCGEGTQVSM
                     KLKKCGFKDIDLICITHLHGDHIFGLLGLLSTIGNSGRTSDLTIVGPVGIVDCIRSMR
                     NLVEYVPYTLKIIENPQGNFSLDNKVLRNLEISTISLEHSIECLGYSFNFRRNPKFDI
                     DKATKNEVPKILWNKLQEGQNIVLDSKQYTPDMVLGELRKGVKISLTTDTRPIESIPD
                     FIKDSDLFICEAMYGDDLDISKAVRNKHMTFREAANLAKLGNVKQLLLTHFSPSLDIP
                     SMYLENATNVFENTILGEDRLSLRLNFDE"
     gene            27622..28506
                     /locus_tag="PC471_001163"
     CDS             27622..28506
                     /locus_tag="PC471_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4846 domain-containing protein"
                     /protein_id="HDF2788301.1"
                     /translation="MKVLKKITILSLITLTLVGCSNGEEKKVVEGSKKTQNSKKDEQP
                     IIVHKDANILKDRYEPPKGFKRVDVEKGSFESFLRNTKLKNYGEKVKYYDGRTKESKG
                     IYDSVFDVDIGNRDLHQCADAIMLMRAEYLYQNKLYDEISFDFVDGFKAKYSKWAQGY
                     RISVKDSGSSWYKATEYSTSYESFRKFMDIVFAYSGTLSLEKELEPVKVEDMKIGDVF
                     IVGGSPGHASIIMDMAENEKTGEKVFMIAQSYMPAQQTQLLINRKNPNLSPWYSLDFE
                     GDLTIPQWTFKRDQLKRF"
     gene            28719..30560
                     /locus_tag="PC471_001164"
     CDS             28719..30560
                     /locus_tag="PC471_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HDF2788302.1"
                     /translation="MKIRRYLILVIIILITTSQSFISYGLERFEEITIFHTNDIHGRY
                     AEGDDHIQIGNLATLKKETPNSILVDAGDCLHGLPIVNMDRGKSAIELIKCAGYDYIT
                     PGNHDFNYGKDRLFELSKSANLGENTLKFLSSNIFENGKKVFDSNDIKEIDGIKIGFF
                     GLSTQETKSKTGFKNVEGLEFKNPIESATEQVNELKGKGANVIVAICHIGTNSLSKPN
                     SIDIANEVQGIDLIIDGHSHTKFENGKQVGDTLIVSAGQYLENIGEIKLTLDTNDVED
                     IKIENKSARLIGKEEALKYKLDSVVSKKVDKIKEQQEKILDKVIGVTTNTLDGSYENV
                     RTKETNLGNLISDILLDKTKADISLFNGGNIRDTIEKGNITRRDIVDVFPFSNTIVTK
                     ELTGAQIKDVLEHGVKLYPEKSSAFLQVGGISYYFNPKQKEGEKITNIQKEGKPLDLN
                     KKYVVATNDYIASGGDEFPCFSKEPVLKEFGNLESAVIEYIEYKKEISKNVDGRIGIK
                     SKDELISKNHESDLILSENEKNINDNEENLSNNIKSSDSDKNKSLDKTSSRESSNLGI
                     SKREAKEKSPKTGDLGFSNSIIIFIVSSTLICLLNFNQKELKDKKSK"
     gene            30865..31572
                     /locus_tag="PC471_001165"
     CDS             30865..31572
                     /locus_tag="PC471_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2788303.1"
                     /translation="MNNILLLEDDKSLNRGISFKLKKEGYNVFSAFGIEEAKNIFAKE
                     EICLIISDIGLPDGSGFDFCEEIRKKSDVYIIMLTALDEEVDIVTGYDLGADDYITKP
                     FSLMVLISKVNALMKRVNTVKNYTLLVCDDLFFYYIENKLIIRTDDKEEEIILSKTET
                     KLLKYLMENSMQTLTKEQLLESLWDSSGNFVDDNTIAVNIRRLRQKVEKNPSEPKYIK
                     TVRGVGYMWGERSIKKC"
     gene            31566..32876
                     /locus_tag="PC471_001166"
     CDS             31566..32876
                     /locus_tag="PC471_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF2788304.1"
                     /translation="MLKNMNLKDGLSVKDDYIMRRVIVYSVLSVLIVTLVFILFEYSR
                     LHKLYEHQIKNTQNVAGILIEKYPNDEIDIVNSIYNSDYSKVSTGEKAFKKFGYGLEN
                     KMSNDKNFSLYLKSFFKESFFIFLMMFIVVIGIIYYFIRYINKRLSKIYFIVDKMSQE
                     NYLKDTTESEETGYLVEYNEYFKEGIFSKINNCLYELNRSLKIKFIKLEKEKESVKSL
                     VTDISHQLKTPLASLKLYNTLLIEEELEEDEANEFLLTNKASINKLENLINSLVNISR
                     LEISMINIKKEDNDIKNTILNAVKSVTPKAKSKSITLNLNEFNSRIIPHDKKWTEESI
                     FNVLDNAVKYTQVNGEINISVEETANFFKIIIEDNGIGIEKSEFNNIFKRFYRGSADE
                     IESVEGSGVGLYLSRKILEEQGGNIIVSSKVGIGSKFSLFLTTM"
     gene            32993..33676
                     /locus_tag="PC471_001167"
     CDS             32993..33676
                     /locus_tag="PC471_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2788305.1"
                     /translation="MKILYTENLSKHYGKGESLVRALDNVDLEINEGEFVAIIGKSGS
                     GKSTLLHMIGGLDIPTSGKVYIDNKNIFTLKEEELAVFRRRKIGFIFQSYNLIPSLNV
                     WENVVLPIGLDGREVDEPFIKELLKSLGLENKHDVLPNTLSGGQQQRVAIARALATRP
                     AIILADEPTGNLDSKTSDEVMSILKSMSKKYSQTLVMITHDDSIAQMADRVIFIEDGR
                     VSKVGDKND"
     gene            33669..36056
                     /locus_tag="PC471_001168"
     CDS             33669..36056
                     /locus_tag="PC471_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HDF2788306.1"
                     /translation="MINTTKIAKSNLKQNKSKSILIIITIILSTLMLSSIGIYIVDAG
                     AYQKENTIKYSGNYQGILANVDEKQADILSNHADVELTGKMNGVGVEKLEDDSNISLA
                     YMNEDALKLNSFEFIKGKLPSKENEIVLDSGALKALGYKNKDLGEKIKISYNDYKSDK
                     KIEKEFIISGILKTSEISEAGKYYYAIISESYMRNTRDMSQEDFNIYVKLNDKSNLSI
                     EQAKSKLNKIANDVGLDTINVAVNENYINALKPDMETIMGGVFVGLVIVLSSILVIYN
                     IFYISIVTKVQEFGKLRAIGATKKQVKNIVFKEGFILAGIAIPIGIILGYVLANIIIK
                     SFMDIDVKSSRLPVILSVVVISFISVVLSLLKPMKVASKVSIVDAVRYTGNKISNKSK
                     RKGYKNINLKRLSHANLERNKKRTYMTLASLILSGTIFITVSTALESFDAEKMAREHF
                     PYDIEVRLSGYEMNSDKNPKDNLNILQMDNPLSKDFFNQIKNMEGIKRIESARSVKIG
                     MEDYDVEFKYDLLQSINENDVKSLNKNIIDGKINLERLQTGDEIVITHVDTAKEMGVK
                     AGDKIRLTLYDGDKKIKKEFKVQAIAMGVPSFGIGKDFIDRTLKYDSTSSLGIYTEEG
                     KYQEIKDSIKKIAKNNGFLETDFIDSRIESNKATISFIKIMGYTLTGIIGVIGFMNLV
                     NTMITSIVTRKKELGMLQAIGLTNKQLVKMLNSEAMYYTSGMMIGSILFGGILGYIAV
                     IFLKKTGLSYATYSLPIVPILLMIVCILIAQFITTYLIGRSFNKESLIDRVRYSE"
     gene            36336..37478
                     /locus_tag="PC471_001169"
     CDS             36336..37478
                     /locus_tag="PC471_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HDF2788307.1"
                     /translation="MNKKINYAPGPTETRENVRLARAEKTTNPDIDIDFVEFYKKTCE
                     KFGNIIGTKNDVYILSGEGILGLEAACASLTEKGDRVLVIDNGIYGEGFKDFVTMYGG
                     EYVLFSSEYTKSIDIDELKAFLDEDSNFKYATVVHCDTPTGVLNDVSKICPLLKEYGI
                     LTVVDSVAGMVGERLSVDESKIDIILGGSQKAISAPAGLTIVGISQDAKNCIKNRKTD
                     VIGFYCNLNIWEGYYEKKYFPYTMPISDIMGLDKALDNILEEGIENVLNRHEKIAYSV
                     RKSIEEYGLELFLEEGYSNTVTAIKIPESIGALELTDYMLKNYNTLVATSLNQYMDTI
                     LRIGHMGENANLNKIEHVLNVLDKSLSALGFKGNGNLLNLFNKYYF"
     gene            37497..38021
                     /locus_tag="PC471_001170"
     CDS             37497..38021
                     /locus_tag="PC471_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HDF2788308.1"
                     /translation="MEVTAKQKFTTKDMVETSLLIALVFIATKFINIRLPISINGGLV
                     HLGTAMLFISAIVFGSKKGALSGAIGMSLFDLISGWTLWAPFTFIVRGVMGYLLGKIA
                     WANGNNGNNFLINVIGICVSSIWMLFGYYVTEVILYGNFIVPLTSIPGNLMQVLIGLI
                     IALPISKVLKKCIK"
     gene            38121..38939
                     /locus_tag="PC471_001171"
     CDS             38121..38939
                     /locus_tag="PC471_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HDF2788309.1"
                     /translation="MLSVTRYSIKNVRLKPWVKFIWYFETKSNILLNNKLLPTDSIDI
                     ILNLSDVMEYKIENQDYIASNIHFNGIRDKHGFIIQHGNIRVIGISFYPFGLYPFLKI
                     PISEFNRQIVDLEAVSKLFAKKLEESLNPTKAIEKVVLCLEEVLLSILEEDLISNKYV
                     KLLNSFIYVNRYSNIKAFCDDTNINIKTLERICLKYTGYTPKILKRIYRFKMASNQLI
                     YNYKDDELFDFIYENEYYDQAHFIKEFKKFSGTSPIKFSGENKTIKENIKYSYL"
     gene            39017..39328
                     /locus_tag="PC471_001172"
     CDS             39017..39328
                     /locus_tag="PC471_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HDF2788310.1"
                     /translation="MKIITEIVEFNVEENLSKEEFIEIVNDLELKFHSIQSGFIDTEL
                     LYDEKSQKWIMIQHWESIEDLKSVSSKMFKDSSTEKFRKALKNQTVKMNIIPQIKSWK
                     L"
     gene            complement(39505..40188)
                     /locus_tag="PC471_001173"
     CDS             complement(39505..40188)
                     /locus_tag="PC471_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2788311.1"
                     /translation="MQKEVAMNKLIKDCVNFFNDCGFSYSICGGYALELFANMHIRPH
                     SDIDIWISDTDKEKSIQYMLNQGWDIYEPLGNYLLRPIIDLNKQHIEQICVFAIKPDC
                     SFIELKKVEDDNYKMNIQCDEQLQFDFIELIYNPQKDGKFLFSQNQRIIRDMDKARLI
                     TSDNIPYMAPEVVLFCKSIYIDRLGYQKDFDVTVPLLSQEQRDWLVNALITAYPDGHA
                     WIDQLANQY"
     gene            complement(40281..40922)
                     /locus_tag="PC471_001174"
     CDS             complement(40281..40922)
                     /locus_tag="PC471_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDF2788312.1"
                     /translation="MGRKKEPDNTIQKILDISHKLFLEKGYEQTTIQDIVDNLDGLSR
                     GAIYHHFKSKDEIIKAITSRLYNDGFYGYEVKNDEGKTALEKLQYRLLHTYEQHNKQD
                     IFLMSSSLLRNPKYLATQFDEIINDIVPWIKALIEEGNQDNSMEVKNPEIAAQMFGFL
                     LNTWCIPTVYPGNPQQMIERFTLFGEQAAFMGISFMNDKIMCEYQKFVNKFSK"
     gene            complement(41096..43099)
                     /locus_tag="PC471_001175"
     CDS             complement(41096..43099)
                     /locus_tag="PC471_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha amylase C-terminal domain-containing
                     protein"
                     /protein_id="HDF2788313.1"
                     /translation="MKSQKRFKILDIDVYLQPFESDIKKRMKHYEDMKSKTLKNYKDF
                     SSFANAHLYYGFHQTNDGFFYREWAPNADSLSLIGDFNNWNRKSHPLRKIGSGNWEIF
                     IPGQNSLPHKSEVKVQVTANGKTFDRIPLYIKRVIQKDFGGFNGQIWQPKTPFIWTDN
                     DFDLKNITSPLIYECHIGMSTESESIGTYNEFTEKILPKIKKAGYNTIQLMAIMEHPY
                     YASFGYQVSNFYAISSRFGTPEDLKNLINTAHSMGIAVLLDLVHSHAVKNTLEGINEF
                     DGSEHQFFHSGSKGNHPAWGTKLFNYGKPEVIHFLLSNIKFWLNEYHFDGFRFDGVTS
                     MLYHNHGLGVSFDSYEKYFSMNTDIEAITYLQFANELMKEIKPNSISIAEDMSGMPGM
                     CIPIKDGGIGFDYRLAMGVPDFWIKTISNLSDEDWDLGKMWYELTTRRPGEKNIGYCE
                     SHDQALVGDKTIIFWLADKEMYWNMEINSNNHVINRAISLIKLIKLITFSLAGEGYLN
                     FMGNEFGHPEWIDFPREGNNWSYKYARRQWSLSEDNNLKYKQLLNFDKSMLELTKYSD
                     IFIQNSHELIHMDNDKKILVYSKGKYLFIFNFHPNLIQSVDISKFQNTNYKTILNTDM
                     VEFGGNTKKENLRDYDVYFSSTPPSSKMPIASRTAIVLLDNGI"
     gene            complement(43252..43890)
                     /gene="recX"
                     /locus_tag="PC471_001176"
     CDS             complement(43252..43890)
                     /gene="recX"
                     /locus_tag="PC471_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HDF2788314.1"
                     /translation="MGIITKIEQQKRNDDRVNIYVDDKFFIAIFKELVYTFNLKKGNE
                     INEEELKPILDDEMYIKAKNKALNILSHADQSEKKLKEKLSSEFDENIIERVIDFLKS
                     YNLVNDSVLAQKIVNTNVNLNKCGKNRIKQNLYNKGINRSTINEAVSELDKDTEFENA
                     MYLAKKRYERVKKEDKKKIYQKISQHLSYKGFDYDIIKRVLNKLLNFDEYDI"
BASE COUNT        17155 a         4811 c         7559 g        14520 t
ORIGIN      
        1 ccatatttat agtatgatat gattataatt ttatataata aataagtaaa aatagtaaaa
       61 aataaattaa gaaatgtttt taataaaaat aagcttaagg gggtcaacca tgagtgcaaa
      121 ggtaacgaat caatatggaa atatagaaat agataatcaa gtaatagctc aagtcgtata
      181 tagagctgca atggaaagtt atggcttagt aggtttagca tataagtcta aaggaatagt
      241 agagctatta aaaggcgaga acgcaactaa aggtgtaaga gttgaggaag tagaagaaga
      301 tgccatagca atcgaacttt atgttataat acaatatggt acaaatatat ctactgttgc
      361 caataatata atagatagag taaaatatgt agttgaaaaa atgactggcg taagagttac
      421 tagaattgat attaatgtac aaggaataag agttaagtaa tttcatagga ggaaacaaat
      481 gattcaatat atagatggta aaaggttaag ggagatgttt atttcaggtg caaacaatct
      541 ccaaaacaat aaagagttag ttgacaagtt aaatgtattc cctgtaccag atggagatac
      601 aggaactaat atgtcattaa caatatctta tgccttaaaa gaattagcaa aagttgaaaa
      661 tgataatata tcagatatcg gaaaagcatt atctaaaggt tctttaatgg gagctagagg
      721 aaactcagga gttatacttt cacaaataat cagaggtata gctaaatcta tagaaggaaa
      781 aagtaaatta tcaacagaag atttagcaaa agcttttaaa aatggttcag acacagcata
      841 taaagctgta ataaaaccaa ttgaaggaac tatacttact gtagttagag aaagtggaga
      901 gtttgcaata aaaactgcga aaaaagagaa agatgtagta aagtttttaa gtatgcttgt
      961 gaaggaatca aattcttcat tagaaagaac tccagacctt ctaaagaatc taaaagaagc
     1021 tggtgtggtt gactctggtg gaaagggtct tgtacttata tatgaaggaa tgttagcttc
     1081 tataaaaggc gataacatag aaataaaaaa tgttgaccta gatactaata tttcaacaag
     1141 tatggatttt gcaaagagta ctactagcac agatgatata aaatattgct attgtacaga
     1201 atttatttta gaaagttcta aggtagaaga tactaaaata agagacataa tgatggctta
     1261 tggtgatagc ctagcagtag ttggagatga tggagtcata aaggtacatg tacacactaa
     1321 tgacccagga aatgtacttc aagaagctct aaagtacggt caattattaa ctataaaaat
     1381 agaaaatatg aagttacaac atgaaaatac attactggat gtagaagaaa agaaagaaaa
     1441 tgatagtgaa cctttagaag aagaaaaaga gtttgggttt atagctactt ctatgggtga
     1501 aggtttggca aatatattta aagatttcgg agttgaccat ataatagaag gtggtcaaac
     1561 tatgaatcca agtactgaag attttatgaa tgctataaag catataaatg ctaaaaatat
     1621 atttattttc ccaaataaca gcaatataat aatggctgct aatcaggcaa aagaattgag
     1681 tgataaaaat ataatagtta tacctacaaa aaatacgcct caaggatttg ctgcattagt
     1741 aacttttaat ggagaattaa gcgaagatga aaataaagaa gctatgatga atgctttaaa
     1801 ctcagttaaa tcaggtcaag taacttttgc tgttagggat acagtgatga atgaaattga
     1861 tgttaaagaa ggtaatataa ttggaattgc agaaggaaat ttattatcag caggagatta
     1921 tgtggatgaa gtaacatcaa accttattga aaagttggtt gatgaagata ctgctataat
     1981 aacattattc tttggtgaag atgttacaga atctcaagca aatgaactac gtacttcttt
     2041 agaagaaaag tttgaagatg tagatgtaga gttatattat ggaggacaac ctctttatta
     2101 ttacttaatt tcagttgaat aatgattata agaacctgta tgaccaagta gcttttataa
     2161 aattggttgt acaggatttt tgtgtagtag gtgatatatt tgttggattt atataaagat
     2221 gttcaatatg ttaagggaat aggacctaaa aaagcagata aattaaataa actgggtata
     2281 tttacactaa aagacctatt gtattatttt ccaagacaat ttgaagatag aaataatcta
     2341 aagaaaatag cacaattaga agatggtgag aaagttacga taaaagcagt aatatcaagt
     2401 ataaatacat ttagcccaaa agaaggtatg actttaacaa agattgatgt aaaagatgag
     2461 acaggctcgg ctaaattagt gttttttaat aagagttata taaaaaatac ttttcgtcca
     2521 ggagattcaa tattagtttt tgggaaggtt aagaaaaaat ttaataactt agaacttaca
     2581 tcatgtgaat tagaatatct tactaattca cctaagaata catgtagatt tatgcctgtg
     2641 taccagctta catacggtgt tacaaataaa gaaattatga gtataattag aacagtactt
     2701 gaagataaag aattaattat acaagaatat atgccacaaa gaattataga aaaatatagg
     2761 ctatgtagta tagattttgc agttagaaac atacattcac caagtagtaa ggaatcatta
     2821 aaaatagctt tatatagaat tgtatttgaa gaattactta tactacaact agggttgttt
     2881 gtatttaaaa gtggcagaaa taaagaagat ggaataaagt ttgaaacaag taaagactta
     2941 aaaaaaataa taagtgcttt accatttaaa ctgactaaag ctcaaaacag ggcattagat
     3001 gaaattatac aagatatgaa tttagagaaa ataatgaata gattggtaca aggtgatgta
     3061 gggagtggta aaacagtagt agctctctta gcacttgcaa attgtgtttt aaatggttat
     3121 cagggcgctt tgatggcacc tacagagata ttagcagggc aacattatat ttcacttact
     3181 gagtctctaa aagattttgg aataaatgta ggtctattaa taggtagtct tacaaaaaaa
     3241 caaaaggata cagtattgga acaaataaag aataatgaga ttgatatatt aataggtaca
     3301 catgcactaa ttgaagataa ggttgaattt aataatattg gtttagttat aacagatgaa
     3361 caacatagat ttggtgttat gcaaaggagc aaactttcat tgaaaggagc aaatcctgat
     3421 atattagtaa tgacagcaac accaatacca agaactttag cacttatact ctatggagat
     3481 ttagatattt ctattataga tgaacttcct ccaggaagac agccaataga aacaattgct
     3541 attgaaaaaa gtaagcgaga tagagcatat aataatcttg taagaagaga ggttgaatca
     3601 ggaagacaag tgtacattgt atgtcccttg gtagaagaaa gtgaagccat agaagccaaa
     3661 tcagcagttg aacttgttga ggagttaaga gcggaatatt ttcacgactt aaggcttggg
     3721 ttgcttcatg gtaaaatgaa gtcaagtgaa aaagatgaag taatgagact ttttaaagat
     3781 aaagaaatag atattttagt ttcaacaaca gtaatagaag ttggtgtcaa tgtgccaaat
     3841 gcaacattaa tgataataga aaatgcagaa agatttggac ttgcacaatt acatcagctt
     3901 agagggcgag ttggaagagg tagtcataaa tcgtattgtg tactaattta tgattctaaa
     3961 acagatgtat gtagacaaag aatggctata atggaagaga ccaatgatgg ttttaagatt
     4021 tcagagaagg atttagaaat aagaggacct ggggaatttt ttgggacaag gcagcatggt
     4081 cttccagaac ttaaggtagc aaacttattt aagcatataa aaatactgaa gttagcacag
     4141 caagaagctc gatatatatt gggtgaagat aataatctac aattaaaaga aaacatggca
     4201 ctcaaaaaag aaattataga taaatttaaa gatacattga aagaaatttc attaaattaa
     4261 ttttaaattg tgttaaaata ttactaatgt aataatgtgc tgaagggaga ttgtcatatt
     4321 gagagtaatt tcaggaaaag cgagaggatt aaaactaaat actccaaaaa atgaagatgt
     4381 aaggccaact acagatagag taaaagagtc tctatttaat atgataaatt catatataat
     4441 ggaaagtgaa gttctggatt tatttgctgg gactggttct ttaggaatag agtgcttatc
     4501 taggggtgcc aagtcatgta cttttgtaga tattagtaag gaaagcatag atattgttaa
     4561 gtccaatata aaaaaggcaa gagttgaaag tgaaagtatt attttaaacc ttgattttaa
     4621 gactgcaata gataaactta aattacaaaa tagtaaattt gacataattt ttatggaccc
     4681 tccgtattat aaaaatatgt ttatagaagc tattgaaaaa attgataatt ctaatctatt
     4741 gaacgaagat ggaataatag ttgtagaaca tgatacgaat gatttatttc cagataagat
     4801 atgtaaattg gagaaaacta gagataagaa atatggaaat actacattaa ctttttataa
     4861 gatggaggca taatatggaa aataaaccta gaaaagctat atttgcagga agttttgacc
     4921 caattactaa tggacattta gatattattt gcagggcttc gaagttattt gacgaattac
     4981 aaataggagt attaaataat cctaataaaa agggtctatt tagctttgat gaaagagtaa
     5041 aacttataga aaaaagtaca agccatttaa ataatataaa agttgtgact tttgatggtt
     5101 tattaataaa ttactgtcag gaaaatggga ttggagcatt agttagaggt gtgagaagtg
     5161 gtgctgatgt tgattatgaa cttcagatgg cacatatgaa tagagagctt aatccagata
     5221 tagagactat aattttacca agttgtacaa aatattcatt tataagttca tctttaataa
     5281 aagaggtttt gctctttgat gctgatataa aaaatttggt tccaaagatt gttttggaag
     5341 aattaaagaa aaaaactagt gggggtaatt gatatatgaa gatagattta gaaatgattg
     5401 aactatttga gcagttagag gagatattta gaagtgcttc tacaatccct ttctcacata
     5461 aatgcacagt ggataaagat gaggttttag caataattaa tgatataaga actttaatac
     5521 cagaagaagt aacacaggca gtatggatta ataaagaaag aaataaaata ataagtcaag
     5581 ctaagcaaga tgctacaaat atagttgaac aagcgaaaaa agaagctgag agaatacaac
     5641 aagaatatca agataatata gaggaattaa agaaaaattc tgaagatgta gtaaaggcat
     5701 atgtagaatc cagtgaacct gttgttcaag cagaccaaag agcaaaagat atagttgata
     5761 gagctgagag aatagccaat gagataagaa ttgggtcaat tgaatatgca gaggatgtgc
     5821 tttctagtgt tgagtataat ttaagagaaa tattagaaga agtaaaaaga gataaagctg
     5881 aacttagacc taaaaaataa attttaaata aataaaaatg gagctatcac acaaaaaatt
     5941 ccaatttatg tgtgatagct ctcttttaat atagcaattt tttgttatat aattgtgctt
     6001 tttagattac ttatatctta ttttgatatt ataatgtagt ttaatctttt atttgttgaa
     6061 atcaacttat ttatttgtat aaataatata ggttattttc aaattttttt aataatttta
     6121 ccactgtctt cttttagatt agagttagac tgtgcaaaat tataaaataa tactaaatca
     6181 tgtgcaatta tttggtatgg cactacatgt agaaaatata gataataaaa ttaaggaggt
     6241 agtatgaagg aaagggctaa aaaaatatta gattttatta ttaaaaataa caatgtaaca
     6301 tcacaagaac tacaagaaaa gtttaatgtt tcaaagagaa ctatttatta cgatattcta
     6361 gctataaatg aacaactagg aaaaagtggt aatataaaaa atgtaaaaca caaatttata
     6421 tttgaaggta atttatgtga tgcaaggaaa ataatttcaa ctgaagaaga taaattttta
     6481 gatagtgatt acagaaaaac ttttatatta aacaagatat tacttggcga aaaaatatca
     6541 atagaaaaac taacaaatga aatgttactt tcaaaaaaca cagtagtaca aacaataact
     6601 gatgtaaaaa aataccttca aacaatgggg ttaagattag agtataaggg aaagtacaaa
     6661 attataggtg atgaatacgt tattagagag ctgtttctga taattgttca agaaaatata
     6721 ttggaaatta attctataag tgaggaagta agtagtttcg atacaaaagg acatataaaa
     6781 cttactgatt attctttatt aaatctaact aagtttgtgg aatttctaaa taaaaggata
     6841 agagatggga aaacattata ctcatataaa tatctaaatg aagctaaaaa aattagttac
     6901 tttagtaatt gtaaagaact tttatgtgaa gaagctaatg aaaatgaaca agcctatata
     6961 tgcacttaca taagttcact tcccagctta aatagtgaag ttaaggaaga tgttgttgaa
     7021 gaatatgtag ataaacttat agataagttt gaagtaaata cagcaataaa attagaaagt
     7081 aagcatgagt ttaagaaaaa tattttaagg cacctccatt cttcttacaa cagaatacga
     7141 tttaagtttc ctattagaaa tccaatgtta gatgagacta agtataaaca tgagtcactt
     7201 tataaaatta taaaatcaat tatagaaaat gaagaagagt ttcctgtatt tgaggggata
     7261 agagaagaag aaataggatt tatagcagcg tattttggag gatatttaag agggtctagg
     7321 gataatggtc taagaagaaa taaagtctta ctagtttgtc ctaatggtct tatggtatca
     7381 aagagtttgg aaatacaact atataaatac ataccaacca tagaaatagt aggtatagta
     7441 tccattaagc aactaaaaga agtaaatgta tactacgatt acataattac aactatagat
     7501 atacaaaatg taaacaacgt aattgttgta aatcctcttt taacatcatc agatgttcaa
     7561 ttacttatga acaaactaat aagtgtaaaa gaaaacgaga agtatttcaa tcttgaatta
     7621 ataatacaag ctataagaaa aaatggagtt atcaataatg aagaagcact aaaagctgat
     7681 ttattaaaca taatacacaa gatagatgaa ggagagatgt atcaaccaat gttaaaggaa
     7741 ttaattaatg cagaaagggt caatataata aaaagtgtta gagattggaa agaagcaata
     7801 aaaatagcct caaagccatt gttagaagat aactctatag aagagttata tatagaaaat
     7861 atgataaagt cagttgagaa atatggacca tatattgttt tagctgatag atttgctcta
     7921 ccacatgcaa gttctaaaga aggagtaaac aagcttgcta tgtctttact aatagtagaa
     7981 gatgaagttg atttattagg aaaaccagta aatatattta tggtattagc tgctgtagat
     8041 aacactactc acattagagc attagcatca ctatcagaga tgatgtacga ggaagaaaat
     8101 gtaaaactaa taattaatgg tgataaaagt tcaatactag aacttataaa taagcaaaat
     8161 taggaggaat gaaaaatgaa gatattagca gtatgtggtt ttggtgtagg ttcatcaatg
     8221 gtattaaaaa tgacaataca aaaggctcta aagcagttag gtatagaatg tgaagtagaa
     8281 aatacagata taaattcagc tagaggtgct gattgtgact gtatattcac aagtcaagaa
     8341 ttgttaaatg aattaaaagg aacttgtaat gtagatgtat atccagttaa aaagtacatg
     8401 gatttagcag aagtcacaga agttgttcaa acttttttaa gtgataaaaa tgataagaaa
     8461 tagtataagt tttaaaaagt aaatttaagt taatcagaat aaaataaaaa cttactaaat
     8521 tcaaaaatag taagaaagag aggagggatt tagtggtaga aaacttaaac agttgaccta
     8581 tcactaaaaa atataatgct tgattttatt tcaaataata ttttacgtaa tccatctatg
     8641 ttaattggat taatagcagc atttggtctt gtactacaga agaagaaagc atctgatgtt
     8701 ataaaggggt cactacttgc agctttcgga attataatta tggagactgg aacaggtatg
     8761 ttagttagtt caatagcacc aattaatgga gctttccaaa gtataagtgg tggtgcagta
     8821 acaaaaggtt taagtgatgt tacttttact gcaagttatg gtggaattat aggtttagca
     8881 atgtttgcgg gtttagctat tcacctaatg atagcaagat ttactcctat taagacaatc
     8941 tttttaacag gtcacatgct ttattggttc ccattcatat ttgtagcttc aggagttgaa
     9001 gctggattaa atggtggagt attaatagct tttgcagcag tattatcagc actctattgg
     9061 tcaataatgc cttggttatt aagaaaatat gtgtttgatg ttataggaga tgattctttt
     9121 acattaggtc atccatctgg ttgtttagca cttatatcag gatttgttgc taaattagta
     9181 ggaaataaag aaaaatctac agaagatata aacatacctc aaagtttatc attttttaga
     9241 gaaatatcta taagtggtgg aatggcagtg tttttaatgt ttatagttgt tgggatagta
     9301 ataccaggtg cgtttaaaga tagtaatgag tttattggaa cgtatgcaat aaagcaaggt
     9361 ctttcttttg gagcaggtct tataataatg cttcatgggg taagaatgct aataaatcaa
     9421 ataatgccag catttcaagg tatatctgaa aaagttgtgc caaatgcact acctgctttt
     9481 gactgtccaa tattatttaa ttataaacca aatgctgtat taatgggatt cttagttgct
     9541 atgctaacat ctacagctat gattttaata tgtaactact ttaatgtatt taatttgcta
     9601 cttataccac ttgttataac ttcattcttt gagtgtggag cagcagcagt aataggagaa
     9661 tcacaaggtg gtatacgtgg agctataata ggtacagcta tagctgcaat atttatggta
     9721 gttttagtag gaatatctgc tgtaatttac tcaggaacta ttcaaaattg gatactaata
     9781 tttggaggta atgacctttc attatttggt ataatcagta ggtttatagg ttcactattt
     9841 ggaggaatat tatagatatg aaatttaaat acatagatga gataagaaga ttaattgata
     9901 taatagaaaa cgaagaaaaa gtcaatatgg aaaaagcagt acaaatcttt gttgaagcaa
     9961 tagaaagtaa aaatgctata ttttcatttg gagcttcaca tgcaggaata ttaacagaag
    10021 aattattcta tagagcaggt ggctgtgttc ttataaatcc agtatttgca aaatctctaa
    10081 tgttagatac agcgcctata actcacacaa gtaagatgga aagattagaa ggatatggta
    10141 cagctctagc tgataaaact ccaattaaaa aaggagatgt agttatagtt cattcagtat
    10201 ctggtagaaa tccagtaagt atagagtttg ctatgaaagc taaggaaaat aaggcaatag
    10261 taatatgtat aactaattta agttactcaa aaagtgtatc ttcaagacat tcttcaggaa
    10321 aaaaacttta tgaagtatgc gatttagtga tagataatca tggtcaaaag ggagatgcct
    10381 gtgtagagat agatggatta aaccaaaaag ttagtccaac ttctactgta atagcaacta
    10441 ctgtgatgaa ctctattata gctcaagcta ctcaggaatt agttaataaa gggctaaaga
    10501 atcctccgat tttctatagt gcaaatattg atggtggaga tgagttaaat aagaagattt
    10561 ttgatgaata taaaagtgta attcattatg aatattaagg ttaaaaaaat atgttaatga
    10621 actttgaagt ttatcaacaa agaatattaa taatatataa taaatgctat catttaaaag
    10681 ctgtaggtaa aaatctacag ctttttatta ttgtagatta aaaagatgcg taattaaatt
    10741 ttaaattgga gaaaataaat tggaaggtat caattataaa tattagaaac gttgtcatag
    10801 aaaaaaacta tattaaaaaa aagtacatag aaaaaactga ttagacaata aatacatgct
    10861 ttgataatat tttaaatgga gtgaaaatgt gtagaattat aatgaaaaaa gaaaataaaa
    10921 atgaaaaaag aaaataaaat ggagggaata aaagtgggta tgttaaaaaa attatgttca
    10981 ctagctatgg tagcaattat gactacaaca cttataacag ggtgttcaag tggaaatgga
    11041 aaagattcaa aaaaggatgg cgaaaaagaa aaactagttc tttggatgcc accagtagaa
    11101 aataatatga aggaagtttg ggaccctatt ttagataaat ttgaggaaaa aaataactgt
    11161 gaagtagatt tacagataat tccttgggat aattattctg aaaaatttgc aacagctata
    11221 agtgctggcg aaggtccaga tgtaggatat atgtatgcag aaatgtatcc acagtttata
    11281 gaaagtggag cagtagaaga tttgactaac tatgcaacta aagaggataa agagcaatct
    11341 atttatataa aaacatctga aatgatgggt ggaatgtatg gtatgccatt ccaagcagca
    11401 aatccagggg tattatacta taacaaggat attttagaga aactaggaga aaaacctcct
    11461 aaaacatggg aagattttaa aagaatttgt aaaaaagcaa cgcaagatac tgatggtgat
    11521 ggaaagatag accaatgggg attagctcag ggatggggag ctaaaacttt tggtaactta
    11581 aactggaact ggtatcctta tttatggcaa gctggtggag atatatttaa tgatgattta
    11641 aaatcggtta aatttaatga taagagtggt ttagaagcag ctaagttctt aaaagaatta
    11701 caagcatatg tgcctgaaga ttctttatct aaagatagta atgaaatgat agaaagtgta
    11761 tttggaccag ggaaagcagc atttacaata atgttatctt ctgctgcaac aagtgtattt
    11821 gatgaatcat tcccggattt aaattgggga tttgtaactg gactagaaga taagaaagca
    11881 gcaacttttg gagcagtaga ccatttatct ttaatctcat ctgcgaaaaa taaagaactt
    11941 gcatacaagt taatacaaca tatgttaagt gttgaatcaa tgacagaatt tcataaagca
    12001 ataccaagag caccaataac aaaaggtgaa ccatatcaag gtgatgaaag attcaaagaa
    12061 atggtggaaa atgacaaaaa tgtatataga ccattagtag taggacctca tggagttgaa
    12121 atatatgaat atttatggaa agaacttcaa actatgattt caggagataa aactccaaaa
    12181 caagcgttag atgatgctgc aaaatattca aatgatttat tagcacaata aaagtttaaa
    12241 gctgtaatat gttgtaaagt actttacaga ttaaaattag cattgtattt ataaatacaa
    12301 tgctaatatt tatattggag aggataaaat ggaaaactta gttagagagt ttgaagaaaa
    12361 ttcaaaaaga aaaaaaacaa aattaaatat taaaccatat atgtatatat tgccattggg
    12421 aataatttta gtggcgtttt atgtgatacc aataataatg tcaatatact ttagttttac
    12481 aaagtataat attattagtc cagctacttt tataggttta gaaaattata aaaaattatt
    12541 tacagatgaa acattaaaag tatctattat aaatactatt aaatttactg ttgttgtagt
    12601 accttgccaa actatattat cattgatttt ggctgtttgg attacaggaa aaggaaatag
    12661 taaaatagct tcatttgcta aaggtgctat atttatacca gtattatcat ctatggtttt
    12721 gataggtatg gtatggaggg ctttattgaa tggagaaggt tctattatat atcaggtatt
    12781 aggtgccttt ggaatagaat catctaaact tttaggagat agtaagacag cattacctac
    12841 acttatgttt atatctatgt ggaaaaatat agggtatttc atggtaattt atatttcagc
    12901 tataatgaat ttaccaaaac actgctatga agtagcgaag gttgatgggg ctacaaaact
    12961 tcaagaattt ataaaaataa ctgtaccttt attgaaacca actacaataa tggttgtatt
    13021 tttagggtcg atatggtcat tacaagtatt tgacttaatt tatactgtga caggtggagg
    13081 tccaggtata tctactatga gtatagttat gcatgcattt aacttaaact ttaaaaactt
    13141 taattctgga tatgcaatga ctgtagcaaa cgtattattt ttgttaatag cagtagtttc
    13201 gattttacaa aataagcttg taaaacgaga caattcagat ttttagaaag gtaggtataa
    13261 tatgaaaaca gcaagaagat tattaagtga tggtttactt ttatttatag cgtgtgcaag
    13321 tttggtacct tttatataca tgttgataat atcattgaag ataacatata actcatatag
    13381 tttggatata tctttttcaa cagtaaccct tcaaaactat atagatattt ttactaaaaa
    13441 aggatttgca caatattttt ttaatacagc aatagtatct ttttcaggtg tattattaaa
    13501 tttagtattt agtacattag caggatattc ttttgcgaag atggatttta aaggtagtga
    13561 taagttattt ttatttatga taatgacttt aattatacct tcccaagtta caatgatacc
    13621 actatatata ataatgaaac acttaggatg gataaatagt tatcttgcac ttattatgcc
    13681 aataccaact gcatttggcg tatttataat gagacaagct attttaggag taccaaaaga
    13741 gttattagaa tcagcaaaga tagatggttg ttcggatttt agaattttaa tacaaatagt
    13801 gttgccttta attaaaccag cattaattac actagcaata tttactttta tgggagcatg
    13861 gaacgagttt atgtggcctc ttatagctac aactaaagat gcaatgagaa cattaacagt
    13921 tggattatct actcttaaga cattccagat aactaattat ggacagatga tggcaggggc
    13981 tacaataaca tttttacctc catttatttt ctatttgatt ttacagagta aatttgttga
    14041 aggtgtgtct ttgtctggta taaaaggtta aatagtctaa tttaataata tataaagaaa
    14101 cttatataaa tatggaggaa tttaaactat ggaaatttca tatgtactga aagattgcac
    14161 attcaaacac aataaatact cagttgtaga agctgaaact tggaaagata aggatttgga
    14221 tattgtagta gctaaaggtg aaaaatttgc atttcaaatt atgttaaaag caacagaaga
    14281 atttaattgt acaattgata aaagtagtgc tatatcatgg aaaggcctgg gaaatagagt
    14341 aaggttagca ttaaatatgc ctaatagttt agaaaataat tttagtataa atatattagg
    14401 atatgttcaa gatgatacaa aggcatttgt gaatgatgct attttaaggg ataaagatgt
    14461 gttagtagag caatatttgc ctcaaacatt ttggattgaa ggtcaagtac cagaagattt
    14521 tgaagaaaat aaccttgata taggtattga tatatttaaa agctttggat atgaagatga
    14581 agaaaaagtt tgtacaattg atgttcctgt aaaagtaaga aatgttgtgc taaagccttt
    14641 agatgaaagc aagttctttt tagatttatg gcaacatcca agctgtttag ctagaatgta
    14701 taaagttgag ttatggtctg atatacattt tgaaatagtc gataactatt taaaagaatt
    14761 agcatcttta ggagaaaaag ttgcaacagt tatagtttca gattatccat gggcagggca
    14821 aagttgttat aaagtttata aaaatccatc aaacttatat gaatataaca tggtaagtgt
    14881 atcaaaagga ttggatggaa agattaaatg taactttgag agtatggata ggtatatagg
    14941 catagctgat aagtataaaa tggctaagga gatagattta tttggtctat taggtaattg
    15001 gtgtgctggt gagtttggaa atcctgtaga aggttataag gaccctataa gagtaagata
    15061 ttttgatgaa aaggataaag tatttaaatt tataaacaat acaaatgatt taaaagaata
    15121 tataggatta gtattaaatc atttaataga gtgtggatta tgggacagag taaggattat
    15181 agctgatgaa ccaaataatc cagaagtagt caaagagtgt atagaattta taaattctac
    15241 tgtaggtact catcaagtta aatataaatc agcaactcac gaccaaaact ttttagacag
    15301 agctaaagat gaaattgatg atatgtcaat aaatttaaaa cttactatac aaaattataa
    15361 agatatagaa agtcttaaga aaaaaatcaa tgacaagggt gggatattga cttggtttgt
    15421 atgctgtttt ccagaaaaac caaatagttt tttaagttct ccttttgtag aaaatagaat
    15481 aataggatgg tatacttatt actttggtct tgatggattt ttaagatggg actataattt
    15541 atggacagaa gacccttgga aagattcaag ttacaaattc cctatttgga aagctggaga
    15601 tatgttcttt gtttatcctg gaaaagattt aaagccagtt agaagtgtca gaatggagaa
    15661 tttaagattt ggaatacagg attttgagtt atttacaatg ttagaaaaag aaaaggtaag
    15721 agagtatata gaagttgagc ttatgcaaga attattaaat aaaaaagaaa atgctgaaat
    15781 taaaggtttt ggtgacatag aactaggata ttcacttgat aattggagat atgataaagt
    15841 taaaaaatat gtattagagt tgttagacag agtataaaaa atataagagt gtctcaaaat
    15901 gaacttttta gttcatgagg cactcttttt gtatgaaatc aatatagttt cattgtttca
    15961 acaatgaaaa ataggattat ttctattcta agacagccct ttagtttttt agttgtattc
    16021 aatgttttaa aactaactat aaacgctata aagtttataa atgacaataa ttatatataa
    16081 catgatgtac tttaatttgc tataataggg attgaataat attatatgga agataacatt
    16141 gtttgggggg attaatataa aaagaatagg tattattata atgttttttt taagtatatc
    16201 ttttattgga ttttatcttg taataagaga taacagtgat acagttaaga tgcaacgagg
    16261 gataaatata ggtaatgcac tagaatcacc taaggatttt ccgtgggatg taaaaatgtc
    16321 aaataagttt tttgatgata taaaagatgc aggttttgac acagtacgta taccagtaag
    16381 gttttctgat tatacgtcag attctgataa ttttaaaatt gatgaagaat tttttaaaaa
    16441 gattgataaa tatgtagatt atgcattgga taaagactta atagttgtgt tggatttaca
    16501 ccattttgaa gaaattatga aagaaccaag agttcataaa gaaaagtttt taaaaatatg
    16561 gcaacagata gctaataggt atcaaaaata tgataaaaaa ttggtatttg aattattaaa
    16621 tgaacctaaa gaaaatttat cttcacaatt actaaacgaa tacattgaag aagcaataaa
    16681 gataattaga aaaactaacc ccaaaagaac tattatagta ggaccatata atttctatca
    16741 aattgattat ttgaatgaat taaatattcc taaagattct aatatagtag tttcttttca
    16801 ctattacgag ccaaatgatt ttgcatttca aggaaatatc tatcataagg gatttgagca
    16861 cttaagcaat attacttggg aaggaacaaa tgaacaaatg gattatttaa aaaaaaggtt
    16921 tgatactgtg gaaaattggg caaataaaaa taaggtaaaa atcttcttgg gtgaatttgg
    16981 aataacaaaa gaggcaccag aagtttcaag aagagcatgg ataaaagctg taagagaaga
    17041 agctgaaaaa cgaaactttt catgggctta ttgggagctt gcatctggat ttggtatata
    17101 caatcaaatt gaagggactt gggatagaga tatattaagt gcattaatag aaaagaggtg
    17161 attttatgaa gaaaaattca aaaaagatac ttataacttt aactattatt actaatgtca
    17221 tatatatatt gtggagaatt ttttacacag tacctaaaga agaagggaag tttgccttaa
    17281 tatgtgccat aatacttctt tttgtagaga taatgggtat gatggaaatg tttgtgcatt
    17341 attatggtat gtcaaatatt gagtatcctg aaaaaccaat cataagtgaa gaactttatc
    17401 cacatgtaga tgtatttatt gcaacatata atgaatctgt tgatttggtt agaaaaactg
    17461 taaatggatg tattcatatg cagtatccag ataagaaaaa ggtacatata tacatatgtg
    17521 atgatggaaa tcgtgaagaa atgcgtattc tagcagaaaa aatgggtgta aattacataa
    17581 ctagaactga aagagaagga gcaaaagctg gaaatttaaa taatgcaatg caacatacaa
    17641 cttctccttt gattgctaca tttgatgcag atatgatacc aatgcatgac tttttaattg
    17701 ctacagtacc atacttttta aaaaatgagc aagctaaaaa agatggagaa aaagaggagt
    17761 atgaaaaggt tggttttgtg caaactccac agagttttta taatccagat ttgttccaat
    17821 ttaatctgca ctctgaagga aggattccaa atgagcaaga ttatttttac agagatattc
    17881 aattggctag aaataggaca aattctgtaa tttatggtgg tagtaataca gttatttcaa
    17941 gggaagcact tgaagaagtt gatggatttt atacttattc tattacagaa gattttgcaa
    18001 caggtatctt aattcaaagt aaggggtata gatgttatgc tattccagaa gtacatgcat
    18061 caggtttatc tcctacagat ttaaaaagtc ttattaagca acgtgaaaga tgggcacgtg
    18121 gatgtataca aactggtaga cgtctaaata ttttgtttag gagaggttta ggtttttggc
    18181 agaaaataag ttatatttct tcaattactt attggtatgc aagtataaaa cgctttgtat
    18241 atataatggc acctatttta ttttctgtgt ttaatgtgat tgtagtaaaa tgtacgttac
    18301 ttcaagtatt ggtattttgg ttgccaatgt atattttaag tagtttatct cttaaaatat
    18361 tttctcaaaa tataagaaat actagatgga ccaatatata tgaaactata atgtttcagt
    18421 ctttaatgcc agcagtaata cttgaaacgt ttgcaatatc taaaaataaa ttttcagtaa
    18481 caaataagag taaattagaa gaaaatagaa tgtataagtt tttacaggga ataccttatt
    18541 ttatttacat ggtcttatct atcataggaa ttttaaaaat gtttgtagca atatttaaaa
    18601 tgagttcaat gacatattca gttgtattat tttggttgat tggaaacctt tttaaccttg
    18661 ttatggctac attatttata tcagggagac agcagttaag aaagtctgag cgttatattg
    18721 ctgaaattga ttttaagtta aagcaaaatt cttatgttct ttcttcaaaa acaattgata
    18781 tatcagaaaa tgggtttgca tttttgctag aaaatccaga atatatttca ccagaagaag
    18841 agtttgaggt agagtttaga gaaaaatctg gaaatgaaat gtatatagcc aatatgaaag
    18901 caaagattgt taatgttgtg gaagtaaatt caaagtggaa atatgcagct tatattacac
    18961 atattgaaga tagtgaaata gataattgga tgtgtattgt tcacgataga atacctactt
    19021 taccaatgac aatatctaat caactaggtt tctttgatga tttgcaaatt aatgtgaaaa
    19081 aacgtattga aaaaactaga acattatcac gaagaagtcc aagaatcaat atgaattttc
    19141 agatggatat aaaaaataca ggtaaattaa gaatagtgaa ttttaattat caatatgtat
    19201 tattaaattt tgagaataaa agtatttatc ctaaagaaat agcattggaa attaatgaaa
    19261 atattgtgtt agaatgtgat ttgtgtgagg gtaaaattga tgaaagaggt atcttatata
    19321 aagtaaataa cattgacagc ataatgcaaa atttcttttt aagagatgaa atgatggatt
    19381 ggatattaca aaataagaca attcttgttt caaagcctag tgaaaagaag gaaaaaagta
    19441 tagatgagtt tgaacccatg gaatatattt aggagagatg aaatgaagac tttaaaagtg
    19501 tttttttgtt ctacaaaata tcttattttg gtgtttattt tttttataaa tactactttt
    19561 ataagttcag caacaagtga taaaatttta gatttgtcct ttaaaaagat agagactgac
    19621 ttaaattcta agattacata tgaagataca ggtgtaaaaa tagaaacgga tagttctaaa
    19681 agcgataagg aacgttattt atatatttac caaaatataa aagaaaattg gagcatgtat
    19741 aataattttt atattgaaat tcaaaataaa aataaaagtt ctcagagaat aaatttaagc
    19801 atacaaagta aaaatatgct tgaatttaga ttaaaagaag gtagtgaagt atttttagaa
    19861 ggtaaaaata taatatattc agataaaata aaagaaggat gcatagaagt accaggtgaa
    19921 tttgaaggca aaatttatgt gaactttaat agccttataa atgaggaaag taatgttgtg
    19981 ctggactcaa atatgctttc aaacattgtt agttggggaa ttacgtttat tccatctgat
    20041 gaagaacata atattgttat tataaaaaag atatctttat tgtctgaaga gaaattaaga
    20101 tttttaaata atattaaaat aattggcgat gaagaagtac aaataccagt attgggtcaa
    20161 agtataagtc agtatgaagt attaggtctt aaaagtgatt ctaaaattaa gtactctctt
    20221 atgggaaaac aagataatgt aagcatatct caaaaaggta aattaacatt aagtaataaa
    20281 gccaaacctg gacaaattat tttacaagtt aatgttgatg ataagtttaa aattggaaag
    20341 aaaataacat taactgagtc ttggagtgtt aataaaaaag ataaggatgg tgttccatat
    20401 acattagtat atcctgagca atcaccaaca gtacaagata tgaaaaaaat aaactttatg
    20461 aataacatca taacgtttgt tcgaatttta tttgtatctc tagtaataat atgctttgga
    20521 atatatttat attggaaaaa atgttcaaaa actaagtaaa gagaggaggt tagattgtgt
    20581 tattttcaag tttaattttt ttattttatt ttttacctat aactttggtt ttatattatg
    20641 tatttaggtt taaccgaacg atacagaaca tgatattgct tgctgttagt ttattttttt
    20701 atgcttgggg agagccaaaa tttgttgtta tcatgatagt ttccataata atgaactata
    20761 tatttggttt actggtagat agatataggg aaagtaaaat aaaagtaaaa gtttttttag
    20821 ttttgatgtg tgcatacaat ataggtgttt tatttatatt taaatatcta gcttttgcgc
    20881 taagaaatat tagtgaactt attagtactg aattgactat acctagtatt gttttaccca
    20941 taggaatttc attttttaca tttcagggaa tgtcttatgt tatagatgta tatcgtagac
    21001 atggagaagt ccaaaagaac cctttttatg tgggattgta tatagcattt ttcccacaat
    21061 taatagcagg tcctatagtt agatatgaga gtgtggcaga acaaattctc aatagaaaag
    21121 aaacttggaa taaattttct attgggactt gtaggtttat aacaggtcta ggtaaaaaag
    21181 tattaatttc aaataacatg gctatagtag cagactatat ttacactatg aatagtcaag
    21241 gagaaatagc tgcatcactt gcatggcttg gttcaatagc ctatactctg caaatattct
    21301 ttgatttttc tgcttattca gatatggcta taggcttagg tcttatgttt ggttttaaat
    21361 ttgaagagaa ctttaattat ccatatatat caaaatcaat tagtgagttt tggagaagat
    21421 ggcacatttc tttgggaatg tggtttaaga gctatatata ctttccgctt ggaggttcta
    21481 gagtagcaaa taaagatatt atgattagaa atatgtttat agtgtggtta tttactggaa
    21541 tatggcatgg agcagaatgg acatttgtaa tttgggggat tttaaacttt gttttcctaa
    21601 ttatagagcg atttataatg tttgaaaaga ttgaaaatca caattttata aaacatattt
    21661 atgctttatt agtagttaat tttgggtggg tattatttag gtcacctaat ttaaaagaag
    21721 catataatta ttttaaagct atgtttggag gcaatggagc tatatggagt gattatacat
    21781 atatgttttt aaaagaatac tgggttttct ttatatttgc atttattttt agtgtaccta
    21841 ttgcaaagaa aatcaataag tttgttgttg aacaagctaa atatagtatg atatttaacg
    21901 tattttatcc aatctcagct attttattgt tttttatttc tgtgacatat ttagtaactg
    21961 gaagctataa tccatttatt tattttaatt tttagttggg aggaaaagta tgaatagatt
    22021 ttttaaagta agattaataa ctgcaattat gttcttaatt attttattcg ctttctctat
    22081 ctttaatgtt tgttattctg gaaaagatac gatacaggaa ataaaaaatt caatagctga
    22141 aaaaagtagt ttaaaagata caatatcatc tattgataat acagtgaatg acaaagtaat
    22201 atacaaaaat acgttcattg aaacttatgg ctatttacaa aaattaatgg gtaaaaatga
    22261 gttttcaaaa tttagtgttg taaaagatac ttctggaaag atgcattata catattttgc
    22321 taaagctcca aatccagttg gtgtgatagc agatagggta agaaaatttt ctgatgaaat
    22381 ggaaaagcaa aacactaaat tggtttacct aatgactcca gataagtata taaaaggagt
    22441 gacaaaattt ccaaaaggaa taccttattc ttatagtaat gagacagcag ataatttttt
    22501 aaatcaacta aaaaagtata atgtggacta tattgatttt agagaaaata ttttaaagag
    22561 tggtattcca aaagatgatt tattttttaa aacagaccat cactggaaag tagaaacttc
    22621 tttttgggca tttggagaat tagttgagca acttaataaa aaatacaata tgaatttaga
    22681 tgaaaatcat tattatagag ataaggacaa ttataattca attgtatatc ctaagtcatt
    22741 tcttggttct atgggaagaa agactggtat cttatatggt ggtatagatg attttacatt
    22801 aatttatcct aaatttaaga ctaattatac ttattataca gattctaaat ctcaaaaatt
    22861 tgaattaaaa ggaaggtttg aagagagttt aatattatca tatcctttta atgcagacct
    22921 tgatttaatg gatggtcaat cagataagta ttttacatat ttattgggga acagaccatt
    22981 agttaaaata aaaaatatag aaaatccaga tggattaaaa gtgctgtttg ttaaagattc
    23041 tctaattgta ccaacagcag catttttttc ctctgtttgt tcatctatag atatgataga
    23101 ccctagatat tatgatggag atatactaga atatgcaaaa tcccatgact atgactttgt
    23161 ttttatatct gtatatcccc aaaatcttac aaaagaattt tttccgtttt gtgagtaaat
    23221 ttctaaaaaa gttttctgat aaaataaatg gctaactggt aaaaagttag ccatttattt
    23281 tgagtgttat tctaagaaat atttttttta ctaaatactt ctttatctat ttttccaagt
    23341 gattttccta cagtaattga tgataccata gcaccattaa catttatcat agtacgtccc
    23401 atatctagta tagggtctat tgcaagtata ccaccaacta atggaaaata agaaccaaga
    23461 ccaactccag agataacaac tgaaacggcc attgtagcag ttcctggaag accagctatt
    23521 ccaaacgaac taattgttat aacaattaac aacataacat aaaagctcat atccatgttt
    23581 actccagcca tattggcaag tgttacagcc attaaagcag gatatatacc tgcacaacca
    23641 ttcataccca tatttgcacc taaacttgct gtaaagcttg atacaccctc atcaactccg
    23701 tgattttgta ctagactttc tatcgttaca ggcaatgttc ctaaactaga acgtgaagta
    23761 aatgccagaa gtaaagcttc agttgaattt tttatataag taatgggatt tagaccattt
    23821 aaagttataa aaattaaatg tataataaat aatattgcaa ctgatatata aagagctaat
    23881 ataaactgta aaacagatac tattgaacta ataccttgtg aagttattgt atttgcaagc
    23941 agagcaacca cagcataagg cataaattta ataacagtca tagacacact tacaattatt
    24001 ttgtaagatg cttctataaa acctataaat ggttttattg tttcaaaatg ttttttactt
    24061 aatctcttta ttgaggtagc tataaatgta gcaaatatta ttgtagctac aatatttcca
    24121 tttgccatag ccattacagg attagatggt agtaatcctc ttaaagtatc cacaagagag
    24181 tttatttctt taagttcttg attttcaact aaagtaggca tattagctcc tacacctaac
    24241 ttcattatat ttgcaacgat aattccaaca attgcagcta tagcagtagt agcaagaagc
    24301 atacctaaag atttaaatgt cagagttcca agattgttat ctttcatatt tataattact
    24361 tttataatgg ataagaaaac taaaggtact actaacattt ttaataagtc cataaatcca
    24421 gaaccaaata ggctatacca cttacttact tcttgtagcc aaatgacttg agttgtatca
    24481 ttaggaaaac cagcaaaagc ttgtattatg attcccaata tgagtcctag tactgtagca
    24541 tatattgttc tcaatgaaaa ttttattttt ttcttttcaa attgtctaac aataaagaac
    24601 gtacctagaa gtaatattat gaaaaaaatt gttttaaaat ctgtaatcat aagaaattgt
    24661 gataaaaatg aactttccaa tatttttctc cttttattta aaaataaatt tttctaagtt
    24721 taaatgatat caatatatta aaaaaatgtc aatattatat cacaatttac ttaaatagag
    24781 taaaacaagt tgattttgaa aaatgtgatt aagtacagaa aaatagggta taataaatgt
    24841 ttaaagaatt tgaattgtgg taagaaaata aaaaatagct tttataagat ttgatttcaa
    24901 attaaaatgt gttaaataaa taactaaaaa ttattataaa aaatgttttc atagaatata
    24961 tatcaaatga aaagcttaaa ataagtgaag aaattaacta gacatcaaag gagagataaa
    25021 atgagagtaa taataattgg tggtgttgca gctggtatga gtgctgcagc aaaattaaaa
    25081 agaataaaac ctgagtatga agttgttgtt tatgaaaaaa ctgaaatagt atcttttggt
    25141 gcttgtggat taccatactt tgttggtgga ttttttaatg atgctgatga attattagca
    25201 agaacaccag agaaacttag agaagctgga attgatttaa atatatttag agaagtcgtt
    25261 gaggttgata gtgaatctaa gaaaataaaa gtaaaaaaca taaaaacaga tgaaatatat
    25321 gaagattact atgataaatt gatgatagca actggagcaa gaagtattat gcctcctata
    25381 aaaaacataa aattaaagaa tgtttctaca ttaaaaagtt tatatgatgg agaatattta
    25441 aaaaaacttt taagtaatga agataataaa agagtgacaa taataggagc tggttttata
    25501 gggttagaag cagttgaggc atgtaagaaa ttaggtaaag atgtagatgt aattcagtta
    25561 gaagatagaa tattgccaca agtatttgat aaggaaatga cggatgtatt agaagaagaa
    25621 ataaatagac ataatgttaa tttgcactta gatgaaatgg ttgtagaact ttgtggagaa
    25681 gataaagtag aaaaagttat aacaaacaaa ggggaaatag atacagatgt agtaataatt
    25741 gctactggcg tgagacctaa taccgagttt ttgtcaaata ctaatataaa aatgctaaaa
    25801 aatggagcta tagtagttga tgagtatgga agaacttctg tagaagatat atactctgct
    25861 ggagattgtg caactataaa gaatatagta agtaatgaaa atgtatatgt tcctttagct
    25921 actggagcta ataaattggg taggatagta ggagaaaacc tagcaggaag agaaatatct
    25981 tatcaaggtt cactatcttc aagctgtata aaaattatgg atatggaggc agcatcaact
    26041 ggaattacag aaaggcaagc taaagattta ggcataaatg tcaaatctaa atttatttct
    26101 gattataatc aaactcatta ttatccagga agaaataaaa tatatgtaaa gcttatttat
    26161 gatgcagata caaaagttat tttaggcgga caagtggctg ggtttaagga tgctgtccaa
    26221 agagcaaatg tattggcagc tgctatcttt ggaaaaatga ccacaaatca acttggaatg
    26281 ttagatttat gttatgctcc accatttgca agaacatggg atgttttaaa tgtagctgga
    26341 aatgtatcta aataatattt agattatagc taatatagtt gacaagcaat taagggaatg
    26401 aatttaccta aactattttt atcatggtaa aattcattcc taaattttta ttccaaattt
    26461 ttattccaaa tttttagaaa taaggattcc attaaattat tcatcaaaat ttaaacgcaa
    26521 gcttaatcta tcttctccaa gaatagtatt ttcaaaaaca ttagtagcat tttctaaata
    26581 catactaggt atgtctaaag aaggactaaa atgagtcagt aaaagttgtt tgacatttcc
    26641 aagttttgca agattagcag cttctctaaa agtcatatgt ttgtttctaa cagcttttga
    26701 tatatctaaa tcatcaccat acatggcttc acatataaat aaatcactat cttttatgaa
    26761 atctggtatt gattctatag gtcttgtatc ggttgtaaga cttatcttaa ctccctttct
    26821 caattctcct aagaccatat ctggagtgta ttgttttgaa tctaaaacta tattttgacc
    26881 ttcttggagt ttgttccata atattttagg aacttcgttt ttagttgcct tatctatatc
    26941 aaatttaggg tttcttctaa aattaaaact gtatcctaaa cactctatag agtgttctaa
    27001 actgattgtt gaaatttcta aatttcttaa aactttatta tctaaagaaa aattgccttg
    27061 agggttttct ataattttta aagtgtatgg tacatattct actaaatttc tcatagaacg
    27121 tatacaatcg actattccaa ctggaccaac tatagttaaa tcacttgtac gaccactatt
    27181 cccaattgtt gataaaagtc ctaaaagtcc aaatatatgg tcaccatgta aatgggttat
    27241 acaaatcaag tctatatctt taaagccaca tttttttagc ttcatagata cctgagtacc
    27301 ttcaccacag tcaattagta tttttctacc tttatagttt ataaatacag aggatagaaa
    27361 tctgtttggc ataggtacat ttccaccaca tccaagtaaa gttaaatcta tcattttttc
    27421 ccctttctaa aattaacaca aatctgttgt ttgtttttat tatgatatcc tattataatt
    27481 attttaacat taaatataac aataaatgtg tatttgagtc aagaaatatt gtcaaataaa
    27541 gtttttatat agcaatgtat aaaaatttgg aaaacgaaaa gtgaaaatta tatttaggag
    27601 tatttaaagg aggaaaattt aatgaaagta ttaaagaaaa ttactatttt gtcattaatt
    27661 acattaacct tagttggttg tagtaacgga gaagagaaaa aagtggttga aggttctaag
    27721 aaaactcaaa attctaaaaa agatgaacag cctataatag tacataaaga tgcaaatata
    27781 ttgaaagata ggtatgagcc accaaaagga tttaaaagag ttgatgtaga aaaaggtagt
    27841 tttgaatcat ttcttaggaa tactaagcta aaaaattatg gagagaaagt aaagtattat
    27901 gatggaagaa ctaaggaatc aaaaggtatt tatgatagtg tatttgatgt tgatattgga
    27961 aatagagatt tacatcaatg tgcagatgca attatgctta tgagagcaga atacttgtat
    28021 cagaataaac tatatgatga aataagtttt gattttgttg atggttttaa ggcgaagtat
    28081 tcaaaatggg cacaaggata tagaatttca gtaaaagata gtggttcaag ctggtataaa
    28141 gcaactgaat attcaacatc ttatgagagt ttcagaaagt ttatggatat agtgtttgca
    28201 tattcaggaa cgttatctct tgaaaaagaa ttggagcctg taaaagttga ggatatgaaa
    28261 ataggagatg tgtttatagt tggtggaagt ccaggacatg caagtataat tatggatatg
    28321 gcagaaaatg aaaagactgg tgaaaaggta tttatgatag cccaaagcta tatgcctgca
    28381 caacaaacgc aacttcttat taatcgtaaa aatccaaatt tgagtccttg gtatagttta
    28441 gattttgaag gtgatttgac aatacctcaa tggactttta aaagagacca gttgaaaaga
    28501 ttttaaaata ataattttat ataataaaag aaaaatatag atgtgaaaaa atgtaattag
    28561 attaaaacta attggatatg gacaaaatta aattgataga taaatattaa aaattacact
    28621 aattaatctt actatagttt aatatttatc taaaaagttt tatgtaaaat ataaatttta
    28681 tattaaagct aaaactaatt tgaggaggct aatttatatt gaagattaga aggtatttaa
    28741 ttttggtaat tataattcta attacaacaa gtcagagttt tatttcttat gggctagagc
    28801 gatttgaaga aataaccatt tttcacacta atgatataca tggaagatat gctgaaggtg
    28861 atgaccatat acaaatagga aatctagcca cattgaaaaa agaaactcca aactcaattt
    28921 tagtagatgc aggagattgt ttgcatggac ttccaatagt aaatatggat agagggaaaa
    28981 gtgcaattga attaattaaa tgtgctggat atgactatat tactcctgga aatcatgact
    29041 ttaattatgg aaaagataga ctgtttgagt tatctaaaag tgctaattta ggagagaata
    29101 ctttaaaatt tttatcatca aatatttttg aaaatgggaa aaaagttttt gatagtaatg
    29161 atataaaaga aatagatgga ataaaaatag gattttttgg actctcaacc caggaaacaa
    29221 agagcaaaac tggatttaaa aatgtggaag gattagagtt taaaaatcct atagaaagtg
    29281 caacagaaca agtaaatgaa ttaaaaggaa agggtgccaa tgtaatagtt gcaatatgtc
    29341 atattggtac aaatagttta agtaagccaa atagtataga tatagcaaat gaggttcaag
    29401 gaattgattt gattattgat ggacacagcc acactaagtt tgaaaatggt aaacaagtgg
    29461 gtgatacatt gattgtaagt gcaggacaat atttagaaaa tattggcgag ataaaactaa
    29521 ctttggatac taatgatgta gaagatataa aaatagaaaa taaatcagct agattaattg
    29581 gaaaagaaga agcattaaag tataagcttg actccgttgt aagtaaaaag gttgataaaa
    29641 taaaagaaca acaagaaaaa atattagata aagttatagg ggtaactaca aatactctag
    29701 atggttcata tgaaaatgta aggacaaaag aaactaattt gggtaattta ataagtgaca
    29761 ttttactaga taaaactaag gctgatattt ctttatttaa tggagggaat attcgagata
    29821 cgatagaaaa aggcaatata acaagaagag atattgtaga tgtatttcct tttagcaata
    29881 ctatagttac caaagaactt acaggagcac agattaaaga tgtattggag catggagtaa
    29941 aactttatcc agaaaaaagt tcagcctttt tgcaagtggg aggaatttca tattacttta
    30001 atcctaaaca aaaagaaggc gaaaaaataa caaatattca aaaagaagga aagccattag
    30061 atttaaataa aaagtatgta gttgcaacga atgattatat agcatcaggt ggagatgaat
    30121 ttccttgttt ttcaaaagag ccagtactaa aggaatttgg aaatttagag agtgcagtca
    30181 ttgaatatat agagtacaaa aaagaaatat caaaaaatgt agatggaaga attggtatta
    30241 aatctaaaga tgagttaata agcaagaatc atgagagtga tttgatatta agtgaaaatg
    30301 aaaaaaatat caatgataat gaagagaatc tatctaataa tataaaaagc tctgatagtg
    30361 ataaaaataa aagcttagat aaaacaagtt ctagagaaag ttctaatcta ggaatatcta
    30421 aaagagaagc taaagaaaaa tctcctaaaa caggagattt aggtttttca aatagtataa
    30481 ttatatttat agtttcaagt acattgatat gtttattgaa ttttaatcaa aaagagttaa
    30541 aggataaaaa gtcaaaataa aaattaatga gcaaaaatta gtagatagta atttaatttt
    30601 agataatagt aaacagcaaa ggaaattagt tatataaata ctaatttcct ttgttttatg
    30661 ttttaacatg attactttta aataaaattg tattttatga aaaataatta tactaaatgg
    30721 catagtatta tggggatttt tagtttttat tcaagtaaat gaaatcatat taacaacttc
    30781 tgatatattt acaaattgat aaaaaaatga ttttattagt atgatataat ttagctagta
    30841 tgtatgataa tggaggatat agggatgaat aatatattac ttttggaaga tgacaaatct
    30901 ttaaatagag gaattagttt taaattaaaa aaagaaggat acaatgtttt tagtgcattt
    30961 ggtatagaag aagcaaaaaa tattttcgca aaagaagaga tatgcttaat tatttccgat
    31021 ataggtctac ctgatggtag tggttttgac ttttgtgagg agattagaaa gaaaagtgat
    31081 gtttacataa taatgttgac agctcttgat gaagaggtgg atattgtcac aggatatgat
    31141 ttaggtgcag atgattatat cacaaagcct tttagcttga tggtattaat atcaaaagtg
    31201 aatgcgctta tgaaaagagt aaatacagta aaaaattata ctctattagt ttgtgatgat
    31261 ttattctttt attatataga aaataaatta attataagaa cagatgataa ggaagaagag
    31321 ataattttaa gtaagacaga gacaaaacta ttaaaatatt tgatggaaaa ttctatgcag
    31381 acattgacta aagagcaact gttagaatct ttatgggata gtagtggtaa ttttgtagat
    31441 gataatacga ttgcagtaaa tataagaaga cttagacaaa aggttgaaaa aaatccatct
    31501 gaacctaaat atataaaaac agtaagaggg gttggatata tgtggggaga aaggagtata
    31561 aaaaaatgct aaaaaatatg aacctcaaag atggtttaag tgtgaaagat gattatataa
    31621 tgagaagagt tattgtatat tctgttttat ctgtactaat agtaaccctt gtatttatac
    31681 tttttgaata tagcagatta cataaattgt atgaacatca aataaaaaat actcaaaatg
    31741 ttgctggaat attgattgaa aaatatccta atgatgaaat agatatagta aattctattt
    31801 ataattctga ttatagtaag gtgtctactg gagaaaaggc atttaagaaa tttggctatg
    31861 gattggaaaa taaaatgtca aatgataaaa attttagctt atacttaaaa tcttttttta
    31921 aggaatcatt ttttattttt ttaatgatgt ttatagtagt tataggtata atatattatt
    31981 ttataagata cataaataaa agattgagta aaatttattt tatagtagat aagatgtcac
    32041 aagaaaacta cttgaaggac actactgagt cagaagaaac tggttatcta gtagaatata
    32101 atgaatactt taaagaaggc atatttagta agataaataa ctgtctatat gagcttaata
    32161 gaagtttaaa gataaagttt attaaattag aaaaagaaaa agaaagtgta aaatcattag
    32221 taacagatat ttcccatcaa ttaaaaactc ctttagcatc cttaaaatta tacaatacat
    32281 tacttataga agaagaatta gaagaagatg aagccaacga gttcttatta accaataagg
    32341 cttctataaa taaattagaa aatttaataa attctttagt gaacatatca aggttagaaa
    32401 ttagcatgat aaacataaaa aaagaagata atgatataaa aaatactatt ctaaatgcag
    32461 taaaaagtgt tacccctaag gctaagagca aaagtataac attaaattta aatgaattta
    32521 acagtagaat tataccacat gataaaaagt ggactgagga gagtattttt aatgtattag
    32581 acaatgctgt aaagtataca caggtaaatg gagaaataaa tatatctgta gaagaaactg
    32641 ctaatttctt taaaattata atagaggata atggcatagg gattgaaaaa agtgagttta
    32701 ataatatatt taaacgtttt tatagaggtt ctgctgatga aattgaatct gtagaaggta
    32761 gtggggtagg attgtattta agtagaaaga ttttagaaga acaaggtgga aatattatag
    32821 tttcttcaaa agtaggaata gggagcaagt tttctttgtt tcttacaact atgtaatatt
    32881 aaatgaaagg aaaatgtaag cctctacatt tattatataa gtataaacag ctccagaaat
    32941 aaaaaattaa taaaatatgg agtaatataa cttagaatga gaggataatt ttatgaaaat
    33001 actatatact gaaaatttat caaagcatta tggaaaagga gaaagcttag taagagcttt
    33061 ggacaatgtt gacttagaaa taaatgaagg tgaatttgtt gcaataatag gaaaatcagg
    33121 ttctggaaaa agtacacttt tgcatatgat tggaggtctt gatataccta catctgggaa
    33181 ggtgtatata gataataaaa atatatttac attaaaagaa gaagaacttg ctgtatttag
    33241 aagaagaaaa atagggttta tatttcaaag ctataattta attccatctc ttaatgtatg
    33301 ggaaaatgta gtgttgccaa tcggtttaga tggtagagag gtagatgaac cttttataaa
    33361 agagctttta aaatcattag gacttgaaaa taaacacgac gttttaccaa atacattatc
    33421 aggtggtcaa caacaaaggg tggcaatagc aagagcatta gctacaagac cagctataat
    33481 acttgctgat gaaccaactg gaaacctaga ttctaaaaca tctgatgagg ttatgagtat
    33541 attaaaatct atgtctaaga agtacagtca aactctagtt atgataactc atgatgacag
    33601 tatagcccaa atggctgata gagttatatt catagaagat ggcagggtgt caaaggttgg
    33661 tgataaaaat gattaatact actaaaatag caaaaagtaa tttaaagcaa aataaatcaa
    33721 aaagtattct aataataatt actataatac tttcaacctt aatgctttct tctattggaa
    33781 tttatattgt tgatgcagga gcatatcaaa aagaaaatac tataaagtat tcaggtaatt
    33841 atcaaggtat attagcaaat gtagacgaaa aacaagctga tatattgagt aatcatgcag
    33901 atgttgaatt aactggtaaa atgaatggag taggtgttga aaaactggaa gatgattcaa
    33961 atatttctct agcatacatg aatgaagatg ctttgaaatt aaatagtttt gaatttatca
    34021 aaggaaaatt gccatcaaaa gagaatgaaa ttgtgttaga tagtggagct ttaaaggcac
    34081 ttggttataa aaataaagat ttaggtgaaa agataaaaat atcttataat gactataaaa
    34141 gtgataaaaa aattgaaaaa gagttcatta taagtggaat cttaaaaact agcgaaatat
    34201 ctgaagcagg taaatattat tatgctatta tatcagaatc atatatgaga aatactagag
    34261 atatgtcaca agaagatttt aatatatatg tgaaattaaa tgataaaagt aatttatcga
    34321 tagaacaagc aaaatcaaaa ttaaataaga ttgctaatga tgtaggattg gacactatta
    34381 atgtagctgt aaatgaaaac tatataaatg ctttaaaacc agatatggaa acaattatgg
    34441 gtggagtatt tgtagggtta gtaattgtat tgtctagtat tttagtaata tacaatatat
    34501 tttatatttc aatagttaca aaggttcaag aatttggaaa gcttagagca attggtgcaa
    34561 ctaaaaaaca agtaaaaaat atagtattta aggaagggtt catattagcc ggaatagcaa
    34621 taccaatagg aataatttta ggctatgtac ttgcaaatat tataataaaa tcatttatgg
    34681 atatagatgt aaaatcatca cgattaccag ttatattatc ggttgtggta ataagcttta
    34741 tatctgttgt tttatcactt ttaaaaccaa tgaaagtagc atcaaaagta tccattgttg
    34801 acgcagtaag atatacagga aataaaataa gtaataagag taagagaaaa ggatataaaa
    34861 atataaatct aaagagatta tcacatgcca atttagaaag aaataaaaaa cgtacttaca
    34921 tgacattggc atcacttata cttagtggaa ctatatttat aacagtatca actgccttag
    34981 aaagttttga tgctgagaaa atggcaagag aacactttcc ttatgatata gaagttagac
    35041 tatctggtta tgaaatgaac agtgataaaa atcctaaaga taatttgaat attttacaaa
    35101 tggataatcc tctaagtaaa gattttttta atcaaataaa gaacatggaa ggaattaaaa
    35161 gaatagaatc tgcaagaagt gtaaaaattg gtatggagga ctatgatgtt gaatttaagt
    35221 atgacctttt acaaagtata aatgaaaatg atgtaaaatc attaaacaag aatataatag
    35281 atggaaaaat aaatcttgaa agacttcaaa ctggagatga aatagtaatt actcatgttg
    35341 atacagcaaa agaaatggga gtaaaagcag gagataaaat aagattaact ttatatgatg
    35401 gagataaaaa gataaaaaaa gaatttaaag ttcaagcaat agctatgggg gttccaagtt
    35461 ttggtatagg gaaggatttt attgatagaa ctttgaaata cgattcaact agtagcttgg
    35521 gaatatatac agaagaagga aagtatcaag aaatcaaaga tagtatcaag aagattgcta
    35581 aaaataatgg atttttagag actgatttta tagatagtag aattgagtca aataaggcta
    35641 ctatttcatt tataaagata atgggataca cgctaacagg cataataggt gtgattggat
    35701 ttatgaatct tgtcaacaca atgataacta gcatagttac tagaaaaaaa gaacttggta
    35761 tgcttcaagc tattggtctt actaataaac aattagtgaa aatgcttaat tcagaggcaa
    35821 tgtattacac ttctggaatg atgataggaa gtattctatt tggaggtata ttagggtata
    35881 ttgctgttat atttctaaaa aagactggat tatcttatgc aacatattct ttgccaatag
    35941 taccaatctt gttgatgatt gtgtgtattc ttatagctca atttataaca acttatttga
    36001 taggacgaag ttttaataaa gaatctctta ttgatagagt aagatacagt gaataaaata
    36061 caaatattta tggttagttt aactagcaaa aaacatataa aaaaatatag ttaaagagat
    36121 tcataattaa ataggttaaa gcatagtaca gagcaaacaa tttaagcaga tgtttgctct
    36181 gtattttatt atgtaatcaa taatatatct aatatactga tattattcat gttagaaaat
    36241 gtaccggtac aattctaaaa aagtataaaa ttgtaccata taaaatgaaa attttgtata
    36301 ataatcgtat agaaaataaa taggagctga tatatatgaa taagaagatt aattatgcac
    36361 caggaccaac tgaaactagg gaaaatgtaa gattagcaag agcagaaaaa acaacaaatc
    36421 cagatataga tattgatttt gttgaatttt ataaaaagac atgcgagaag tttggtaata
    36481 taattggaac aaaaaacgat gtttacatat tgagtggtga aggaatatta ggtcttgaag
    36541 cagcatgtgc atcacttaca gaaaaaggag atagagtact tgtaattgac aatggtatat
    36601 atggagaagg ttttaaagat tttgtaacaa tgtatggtgg ggagtatgtg cttttttcaa
    36661 gtgaatatac taaaagtata gatattgatg aattgaaagc atttttagat gaggatagta
    36721 attttaaata tgcaacagta gtgcattgtg atactccaac tggcgtatta aatgatgtaa
    36781 gcaaaatatg tccattactt aaagaatatg gaatattaac tgtagttgat tctgtagcag
    36841 gtatggtagg agaaagatta agtgtagatg aatctaaaat agatataata ttaggtggtt
    36901 cacaaaaagc tatttctgca ccagcaggac ttactatagt cggaataagt caagatgcaa
    36961 aaaattgtat aaaaaataga aaaactgatg taataggttt ttattgtaat ttaaatatat
    37021 gggaaggcta ttatgaaaaa aaatacttcc cttatacaat gccaataagt gacataatgg
    37081 ggcttgataa agcacttgat aatatattag aagaaggaat agaaaatgta ttaaatagac
    37141 atgaaaagat agcttatagt gttagaaaat ctattgagga atatggtcta gaactatttt
    37201 tagaagaagg atattctaat acagtcacag ctataaaaat tcctgaaagt ataggagctt
    37261 tagaacttac tgattatatg cttaaaaatt ataatacact tgttgcaaca tcactaaatc
    37321 aatatatgga tactatttta agaataggtc atatgggaga gaatgcaaat ctaaataaaa
    37381 tagaacatgt actaaatgtt ttggataaaa gtttaagtgc attaggattc aaaggaaatg
    37441 gaaatttgtt aaatttattt aataagtatt atttttaata gataggggga tatagtatgg
    37501 aagttacagc taagcaaaaa tttacaacga aagacatggt ggaaacctca ctattaatag
    37561 cattagtatt tattgcaact aaatttataa acataagatt accaatatct ataaatggtg
    37621 ggcttgtgca tttaggaact gcaatgttgt tcatatcagc aatagtattt ggaagtaaaa
    37681 aaggtgcttt atcaggagct attggtatgt cactttttga tttgatatct gggtggactt
    37741 tatgggcacc gtttaccttt atagttagag gagttatggg ttatttactt ggaaagattg
    37801 catgggcaaa tggtaataat gggaataatt ttttaatcaa tgtaatagga atatgtgttt
    37861 catcaatttg gatgctattt ggatattatg taacagaagt tatactttat ggtaatttta
    37921 tagtacctct tacttcaata ccaggtaatt taatgcaagt attgataggt cttataatag
    37981 cgcttcctat ttctaaggta ttgaaaaaat gtataaaata aatataaagt gaactaaaat
    38041 ttatgatagc ataaattata ctgtataata ttaggatgga gtacataatt aaaaaatttt
    38101 agtatagagg ttagttcaca atgttatcag ttacaagata ttcaatcaaa aatgttcgtt
    38161 taaagccgtg ggtaaagttt atttggtatt ttgagacaaa atcaaatata ctgttaaata
    38221 ataagttact tccgacagac agtatagata ttattctcaa tctttctgat gttatggaat
    38281 ataaaattga aaatcaagac tatattgcta gtaatattca ttttaatgga ataagggata
    38341 aacatggatt tattattcaa catggaaata tacgtgttat agggatttct ttttatccat
    38401 ttggtctcta tccattttta aagataccaa tttcagagtt taacagacaa attgttgacc
    38461 tagaagcagt atcaaaacta tttgcaaaaa aattagaaga gtcattaaat ccaacaaaag
    38521 caatagaaaa agttgtttta tgtttagaag aagtattatt atccatatta gaagaagatt
    38581 taatttcaaa taaatatgta aagcttctaa attcatttat atatgttaat agatatagca
    38641 atataaaagc attttgtgat gatacaaata taaatattaa aacattggaa agaatctgtt
    38701 taaaatatac agggtataca cctaagattt taaagagaat ttacagattt aaaatggcaa
    38761 gcaatcaact tatttataat tataaagatg atgaactttt tgattttatt tatgaaaatg
    38821 agtactatga ccaagcacac tttataaaag aatttaaaaa attttcaggg acgtcaccaa
    38881 ttaaatttag tggtgagaat aagacgatta aagaaaatat aaagtacagc tatttataat
    38941 tatactgtcg attttttaca atacctccaa tttatttttt gatatgatat aggaaaataa
    39001 attggaggtt ttacatatga aaattattac agaaatagtt gagtttaatg tggaagaaaa
    39061 tttatcaaaa gaagagttta tagaaattgt aaatgactta gaattaaaat ttcattctat
    39121 tcaatctggt tttattgata ctgagctttt atatgatgaa aagtctcaaa agtggattat
    39181 gatacaacat tgggaatcta tagaagacct caagagtgtt tctagtaaaa tgtttaaaga
    39241 ttcttcaact gagaaattta gaaaagcttt aaagaatcaa actgttaaga tgaatataat
    39301 accacaaatt aaaagttgga aattatagaa ctgaatgtaa aagatagtta aatagtatca
    39361 aactaaatta gccaaataac attttgtatt taaattgtta tttggctttt agtgtttatt
    39421 ttaatatgag tgttatatac tagaaaaacc aacaaactgt tagagtgtgg attatcataa
    39481 tgtcatattc gggagctaaa aggcttaata ctgatttgcc aattggtcaa tccatgcatg
    39541 tccatctgga tatgctgtta ttaaagcatt tacaagccaa tccctttgct cttgtgataa
    39601 tagtgggact gttacatcga agtctttttg ataaccaagc ctatctatgt atattgattt
    39661 gcaaaacaaa accacttcgg gagccatata tgggatatta tctgatgtta tcagtctggc
    39721 cttatccata tctcttataa ttctttgatt ttgggaaaaa agaaattttc catctttctg
    39781 tggattatat atcaattcaa taaaatcgaa ctgtaactgt tcgtcacact gtatattcat
    39841 tttatagtta tcatcctcga ctttttttaa ttcaataaat gagcaatcag gcttaatcgc
    39901 aaaaacacaa atttgctcta tatgttgctt gttaaggtcg ataataggtc gtaataaata
    39961 attacccaag ggctcatata tatcccatcc ttgattaagc atatactgaa tgctcttttc
    40021 tttatctgtg tcagaaatcc atatgtcaat atcactgtgt gggcgaatat gcatattcgc
    40081 aaataattct agggcatatc caccacatat tgaatatgaa aagccacaat cattaaaaaa
    40141 gtttacacaa tctttaatta gtttgttcat agcaacctcc ttttgcatgt actgaaataa
    40201 attaaatttt ttaaaatacc aactaaatgt ttgttttgtg taaatataaa actgccgata
    40261 ttaaggcagt tttatattta ctatttagaa aatttattaa caaatttttg atattcacac
    40321 attatcttat cattcataaa agaaataccc ataaaagcag cttgctcacc aaacaaagta
    40381 aaacgttcta tcatctgttg tggattacct ggataaactg ttggaataca ccatgtgttt
    40441 agaagaaaac caaacatttg agcagcaatc tctggatttt taacttccat agaattatct
    40501 tgatttcctt cttcaatcaa tgcttttatc caaggcacaa tatcatttat aatctcatca
    40561 aactgcgtag ctaaatactt aggattacgc aatagtgatg atgacattaa aaaaatgtct
    40621 tgtttattgt gctgttcata agtatgaagc aacctatatt gtaatttttc taatgcggtc
    40681 tttccttcat cattttttac ctcataccca taaaatccat cattatacaa ccgacttgtg
    40741 attgccttaa ttatttcgtc ttttgattta aagtgatgat aaatagcacc tcttgaaagg
    40801 ccatctaaat tatctacaat gtcttgaatc gttgtctgct catacccttt ttctaagaat
    40861 aacttatgtg aaatatcaag tattttttgt atagtattat caggttcttt ttttcttccc
    40921 aaaaaactca ctcctcgaaa caatcatttg gtatgtttat cttagcaaat acataatgat
    40981 ttgtcaatgt gtctttttag catgttgaac aggtatattg ccatgctttc attattttat
    41041 ttttttgcct tatactggac tatatttgca gtttcctaat atatttggct catatttaaa
    41101 taccattatc taaaagcact attgctgtac gagaggcaat aggcatttta gaagaaggag
    41161 gagttgagct aaagtataca tcataatctc ttaagttttc tttttttgtg tttcctccaa
    41221 actctaccat atcagtattt aatatcgttt tgtagtttgt attttgaaac tttgaaatat
    41281 caactgattg tattagatta gggtggaaat taaatataaa taaatatttg cccttactgt
    41341 acaccaaaat ctttttatca ttatccatat gtataagttc atgactattt tggataaaaa
    41401 tatcactgta ttttgtaagt tcgagcatag atttatcaaa atttaataat tgtttatact
    41461 ttaagttgtt atcttctgaa aggctccatt gtcttctggc atacttatag ctccaattgt
    41521 tgccttctcg tggaaaatca atccactcag gatgtccaaa ttcatttccc ataaaattta
    41581 gatatccttc acctgctaga gaaaaagtta ttaatttaat taatttaata agagatattg
    41641 ctctattgat aacatggtta tttgagttta tttccatatt ccagtacatt tccttgtcag
    41701 ctagccaaaa aataattgtt ttatcgccaa ctaatgcttg gtcatgggat tcacaatatc
    41761 ctatattttt ttctccaggt cgtctagtag taagttcata ccacattttg cccaaatccc
    41821 agtcttcatc agataagtta gagatagttt ttatccaaaa atcaggtact cccattgcga
    41881 gtctatagtc aaatccaatt ccgccgtctt ttatagggat acacatacca ggcattccac
    41941 tcatatcttc tgcaatactt atcgaattag gtttaatttc tttcattaat tcatttgcaa
    42001 attggagata tgttatagct tcaatatctg tattcatact aaaatatttt tcataactat
    42061 caaatgaaac tccaagacca tggttatgat ataacattga agttacacca tcaaacctaa
    42121 agccatcaaa atggtactca tttaaccaaa attttatatt tgaaagcaaa aaatgtatta
    42181 cttcaggttt accatagtta aaaagttttg taccccaagc aggatgattt ccctttgaac
    42241 ctgagtggaa aaattgatgt tcacttccat caaactcatt tattccttct aaagtattct
    42301 ttacagcatg agaatgaact aaatctaata aaacagcaat ccccattgaa tgagcagtgt
    42361 ttatgaggtt ttttaagtct tcaggagtac caaacctaga tgagattgca taaaagtttg
    42421 aaacttggta tccaaaagat gcataatatg gatgttccat aatagccata agctgaatgg
    42481 tattgtatcc agcttttttt atcttaggaa gtattttttc agtaaattca ttataagttc
    42541 caatagattc gctttcagta gacataccta tatgacattc atagattagt ggagaagtta
    42601 tgttttttaa gtcaaaatca ttgtcagtcc agataaatgg agtttttggt tgccatattt
    42661 gtccattaaa tccaccaaaa tctttttgaa ttactctttt tatatataat ggtatacggt
    42721 caaatgtttt tccatttgca gtaacttgta cctttacctc tgatttgtga ggtaagctat
    42781 tttgaccagg aataaatatt tcccaattac cagacccaat ttttcttaga ggatgagatt
    42841 ttctattcca attattaaag tcaccaatta gtgacaaact atctgcattt ggagcccatt
    42901 ccctatagaa gaatccatca tttgtctgat gaaatccata gtaaagatga gcatttgcaa
    42961 aagaactaaa atctttatag tttttcaatg tttttgattt catatcctca taatgtttca
    43021 ttcgcttttt tatatcagat tcaaatggtt gtaggtatac atcaatatct aaaattttaa
    43081 atcttttttg acttttcatt gtatttctcc tccataataa atattacatc taaaaaataa
    43141 tatttatctc atactatctt ctctgtataa ttgatatata ttatataatc ctaataaaaa
    43201 gcttaagcca ccaatgatta atttcataat ttacctccct agtatttata cttatatatc
    43261 gtattcatca aaatttaaaa gtttgtttaa aactctttta ataatatcat aatcaaagcc
    43321 tttataggaa agatgttgag aaattttttg atatattttc tttttatctt ctttctttac
    43381 tctttcatac cttttttttg ctagatacat agcattttca aattcagtgt ccttgtctaa
    43441 ttcagatact gcttcattga ttgtacttct attgatacct ttattatata aattctgctt
    43501 aattctgttt ttgccacatt tatttaggtt aacatttgtg ttaacaattt tttgagctaa
    43561 tacactgtca tttacgagat tataactttt aagaaaatct atgaccctct caattatatt
    43621 ttcatcaaat tctgatgata gtttttcttt taattttttt tcagattggt cagcatgaga
    43681 taaaatattt aatgctttat ttttagcttt tatatacatt tcatcatcta aaataggttt
    43741 taattcttct tcatttattt cattcccttt ttttaaatta aatgtataaa ccagttcttt
    43801 aaatatagca ataaaaaatt tatcatctac ataaatatta actctatcat catttctttt
    43861 ttgttgttct atttttgtaa ttatacccat aagtcctcct tttttatatg taattatatt
    43921 aacaaaagca actaataaaa tttaacaaaa aacttatatt tgtatgttat attttatcaa
    43981 aattaagaca aaatagtaaa aagttatttt tattaaaaag taaagcagaa ttatgttaaa
    44041 taatt
//
LOCUS       DAMJYR010000023        44016 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.023, whole genome shotgun sequence.
ACCESSION   DAMJYR010000023 DAMJYR010000000
VERSION     DAMJYR010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 44016)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44016)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44016
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.023"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            192..437
                     /locus_tag="PC471_001177"
     CDS             192..437
                     /locus_tag="PC471_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03905 family TSCPD domain-containing
                     protein"
                     /protein_id="HDF2788315.1"
                     /translation="MKYKTTGTCATEIEFEVKENKVTNVNFIGGCDGNLKGLKVLVEG
                     MNIEDVIQKLKGIECKTKPTSCPDQLSLALENYMNIK"
     gene            472..1197
                     /locus_tag="PC471_001178"
     CDS             472..1197
                     /locus_tag="PC471_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HDF2788316.1"
                     /translation="MKPIIGILGNLIIMENGMFPGLERSYVNNDYINAVLKGGGSPVI
                     IPVNTDKEVIKKQIEMVDGVLISGGWDINPQLYGEETREETTFIYPEVDEFDLIAISI
                     ALELKKPILGVCRGLQILNVSLGGTLYQDNNLIEGSYIKHTQSSKRHVATHKVDVKEG
                     SILEGILGKQLLTNSYHHQSVNQLGKGLKAIAYSKDGIIEAIEKEDENFVVGVQWHPE
                     MMVDYCDKMEKLFKYFINICSNL"
     gene            1219..2532
                     /locus_tag="PC471_001179"
     CDS             1219..2532
                     /locus_tag="PC471_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDF2788317.1"
                     /translation="MNEKNKMGLISIILLGINAVVGAGVFLLPGDAMKSFGVASIFVY
                     IFDMLLVLSMAFCFAEVAGKFNKNGAAYVYTKEAFGDFCGFEVGLMKWVIGCISWGAL
                     IVGFPTSLSAVWAPAGEPHIQKIIIVAMIVGLTIINLLGVSLSKIVQNVITVGKLIPL
                     ILFIGIGIFFIKGVNFTSSTMVPPGAGATEFGAAALLMFYSFTGFESIAVAAEDMENP
                     QKNIPIAIISVIVIASIIYILNQVVCVGILGDSLSSTSTPVADAARICFGNMGAGLVT
                     FGTLVSVGGICMCGAFVNPRSCVALADDKMLPRIFARKDKKGTPYVAIIATMLITIPI
                     ALSGSFAELAAISAVARFIQYIPTSLSVLVFRKKRPELVGTFKTPFGAVIPLIAVCVG
                     TWLLFQASMHQLIMGLGALAIGVPLYFIMKSYNRKIYGENLKKIV"
     gene            2738..3277
                     /locus_tag="PC471_001180"
     CDS             2738..3277
                     /locus_tag="PC471_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDF2788318.1"
                     /translation="MPTITLKNGVDVLIREGVREDAQSIIDFYNEVGGETHFLSFGKD
                     EYKISLEEQENAIESAKASDNSVKLIAFIDGEIVGIATIDSNQKAKGKHVGVLGIVVK
                     EKYWGIGLGKRLMLDLIEWCKSNGITKKITLVTNEENYNAIGLYKKVGFEVESILKKE
                     CYYNGVYTDLIGMSLLLGI"
     gene            3366..5351
                     /locus_tag="PC471_001181"
     CDS             3366..5351
                     /locus_tag="PC471_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1,6-bisphosphatase"
                     /protein_id="HDF2788319.1"
                     /translation="MKNLCKISDDYLNSKLKYLKLLSKQYPSISKASTEIINLEAILN
                     LPKGTEHFITDVHGEYEPFVHVLKNGSGVIKRKIEELFSNTIRDSEKKMLATLVYYPE
                     QKLDLIIKQEENIDDFYRINIYRLIELCKYASSKYTRSKVRKLLPENFKYIIEELLHE
                     HVKSEHKEEYYKSIVETIVDIGIAKEFIIAISTVIQKLVVDRLHVIGDIYDRGPRPDI
                     IVDKLIEHHCVDIQWGNHDILWMGAASGEKTCIANALRISARYANLDIVEDIYGINLL
                     PLATFAIEMYKDDPCKEFIPKVNDQSVTTTEKSLMAKMHKAISIIQFKLEGEVIRRRP
                     EFEMEHRLLLNMINYDEGTINLKGKTYKLKDTYLPTIDKKDPYKLTMEERNVIDKLVS
                     SFRGSEKLQKHVSFLFSKGSIYLKANSNLLIHGCVPLNEDGSFMSMNIMGKEYKGKAL
                     MDKMESLAREGFFFKDKAEEKLYGMDIMWYLWTGKCSSLFGKDDMTTFERYFIAEKET
                     HKENKNPYFKLRENEMACKRLFEEFDLELDESHIINGHVPVESKNGESPIKANGKILV
                     IDGGFSRAYQKTTGIAGYTLIYNSRTLQLVSHEPFNSAEEAIANESDILSTTVVVEHK
                     AKRKMVRDTDEGIKIQEEIEDLKLLLMAYKKGLIKEM"
     gene            5575..6543
                     /gene="spoIIIAA"
                     /locus_tag="PC471_001182"
     CDS             5575..6543
                     /gene="spoIIIAA"
                     /locus_tag="PC471_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AA"
                     /protein_id="HDF2788320.1"
                     /translation="MNKLSDEIINSLSTTIREKVEKVSNNNLNIEEIRLRSQKPLILN
                     ANSKDYFYNQKTMTLDLNQQNSYVVTREDVEQTFQIICKYSIHSFMDDIKKGFITLRG
                     GHRVGLVGKAIVEDGQVKNIKHISSLNIRVSREIIGCSDKILSHIIKGKNQINNTLII
                     SPPQCGKTTLIRDIVRNLSNGNEDYGFKGLKVALVDERNEIAGAYLGVPQMDVGIRTD
                     IIETCPKDLGITMLLRSMSPNVIVTDEIGSEKEIKALYTALNGGIGLITTVHGDSIED
                     IQNRKELNRLLDKELFKKVIILSAKRGAGTIEKIYDLEEKRWYFAN"
     gene            6533..7054
                     /locus_tag="PC471_001183"
     CDS             6533..7054
                     /locus_tag="PC471_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AB"
                     /protein_id="HDF2788321.1"
                     /translation="MQIKIIIIAFLIGSSYLIGEQIYKTYTRRHKQLNDLIRVLEILR
                     MDLSFGLYTLEEIFNRIGGNKEFCFWKFFYQISEGLHNEQSKTLEIIISENIDVLSKE
                     TYLGNKEIEELKNLILTLGKSDIESQQRMIDLSIENLKKQTYETKEDINKKGVLYKKL
                     VTFIGIGICIILI"
     gene            7071..7268
                     /gene="spoIIIAC"
                     /locus_tag="PC471_001184"
     CDS             7071..7268
                     /gene="spoIIIAC"
                     /locus_tag="PC471_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018589775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AC"
                     /protein_id="HDF2788322.1"
                     /translation="MEISLILKVAGVGILISVLNMILEKTDRKDWAGLTTLAGVIIVL
                     GMVITEISDLFNTVRTMFQLY"
     gene            7284..7670
                     /gene="spoIIIAD"
                     /locus_tag="PC471_001185"
     CDS             7284..7670
                     /gene="spoIIIAD"
                     /locus_tag="PC471_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AD"
                     /protein_id="HDF2788323.1"
                     /translation="MEIMQLIGIAIISTTLCLVIKKDRPEIANFIAIITGVIILLSVM
                     FKLNFIVDSIQDLANKANIPTMYISLIIKLIGIAYLMEFAIQLCKDCGEGNIASKLEF
                     GGKIIVMSMSFPILLSIVEMVVNIIP"
     gene            7755..8960
                     /gene="spoIIIAE"
                     /locus_tag="PC471_001186"
     CDS             7755..8960
                     /gene="spoIIIAE"
                     /locus_tag="PC471_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AE"
                     /protein_id="HDF2788324.1"
                     /translation="MKKRFLPMIIGFLFTFFFVNIFAIMIFANEVPSSEDKEYGETKN
                     SIDKYIDGQLDKLDINEIQDYINKEIVINDVNLKSFVKDLISGEKNILDLFNKDGLKI
                     LMFDEFKASLKVVAVILVLALLSSILKSLENSFSSGAVSQIATYIIFITMVSLTLIGF
                     KDVLQICYDAIDHTVGLMQVIMPILITFLLLIGFPITSTTLNPIFIGGVTFINVFFKN
                     FLFVSITVAFGILIINNLSKNIRLKRFFSFVKQINYVSIGAMFTVYLGLVSIQGLYVT
                     SFDKFSVKTAKFAIGNFIPVVGGFVSDSVDILLSSSQLIKNIFGGIGLILLVGICLLP
                     VIKILSVIVVYKLAAIIVEPVGEDGISNFLNEVANLMIIMLASVIAITVMFFVTVAIL
                     TSISVVSQG"
     gene            9020..9637
                     /locus_tag="PC471_001187"
     CDS             9020..9637
                     /locus_tag="PC471_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AF"
                     /protein_id="HDF2788325.1"
                     /translation="MLEGIKAWIVSVLIGAFIVNIVDMILPSSKIKPYVNLVLNFMFV
                     FIVITPVVGFFSKDMSLEDRILKSMGNYNKQYVDSTNALAKETGNNSLSKGYEDGLKE
                     VLKLKLDEYGYDLEDIELNGANINNIKIKEKNNSAKSNNSSNEENKNNSTKREEENIN
                     SNDKENSKQVFKKGTEYGLNLNEEKLKNDLIKVLDVSIEDIQIDK"
     gene            9649..10203
                     /locus_tag="PC471_001188"
     CDS             9649..10203
                     /locus_tag="PC471_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AG"
                     /protein_id="HDF2788326.1"
                     /translation="MLKNLNDKDKRKVYSLITIAGICVVSLVLLSCFPSSKSDKQVGK
                     TETNKNAEKQVTKEQEKDDLESKLTAILSKIDGAGDVDVMVTFESSEEIQPAFNSNNT
                     TETTEEKDAQGGERTVTTSSENKTMITSNSSDPVVIKTTEPKIKGVIVVASGASDPSV
                     KETLYSAVQTSLQVAGHQVEIYSK"
     gene            10230..10919
                     /locus_tag="PC471_001189"
     CDS             10230..10919
                     /locus_tag="PC471_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIIAH-like family protein"
                     /protein_id="HDF2788327.1"
                     /translation="MKFNYKGRGFVIITLTAMLVVVGTVNYQLSKKSLLETSKEFKAY
                     EEAQLQKNTDESDSSKTEDSSNVDKQDGKESADIDIVDSKASKVKEKATETSKEIKAQ
                     LSSEKNMKKASYILDMKMNREKQRNELVQDLNEMINNPSTTEESRKEASNMKLNIVKI
                     QEKELQIENLLSTKGYEEALVYISDNKVNVVVNEAKLEKKDAAKIFDLVAEQANVKYE
                     NIKLTNNNSNK"
     gene            11029..11391
                     /locus_tag="PC471_001190"
     CDS             11029..11391
                     /locus_tag="PC471_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HDF2788328.1"
                     /translation="MEDNKFGQVKISNDVIATIAGLAALEVEGIETTATLTDKLLKNN
                     GVKIQIEEEDVNLDVMVTIKYGMSIPDTAFKVQENVKNTVETMTGLKVSQVNIHIQGI
                     SFKKDKVDKEEAKATKKN"
     gene            11476..11982
                     /gene="nusB"
                     /locus_tag="PC471_001191"
     CDS             11476..11982
                     /gene="nusB"
                     /locus_tag="PC471_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HDF2788329.1"
                     /translation="MKKDRAQKSTTREYIMKLIYQININKEDFETLEDKVDNFLKDNS
                     EHIINRYKELALQYSKNTNLKLEDTEIEDVIDKKYINTVCKALKENHDKIDELINKHA
                     KNWTVDRMPKVDVSILRLSVCEILYLDTPNKVSINEAVELAKIYCDDKSPKFINGILG
                     SVVDEIGK"
     gene            11966..13036
                     /locus_tag="PC471_001192"
     CDS             11966..13036
                     /locus_tag="PC471_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-sialoglycoprotein endopeptidase"
                     /protein_id="HDF2788330.1"
                     /translation="MKLGNNIIIGIDTSCYTTSIAAISLDKKVIFNEKIMLEVRDNSK
                     GLRQSEAVFQHINNLGILSDRIKSFKDKFNVEGVCSSKKPRPVENSYMPVFNVGHNFG
                     KLLSSIYGCRFYETTHQENHIEASLLNSKLKNNNKFISVHMSGGTTEILLTSKQDSHH
                     NVCDTNLGKIAKISIKKDDKSKLYNNFGYNIDIIGGSKDISFGQLIDRVGIKLGYKFP
                     SGKYLDENALNCNLKIESGLKTSVRDGYMNLSGLENQVNKIINDNGDNTNQKEYISKL
                     VLDSVVRNMFKSLVYLCETYNVNEVVFAGGVSASKYILRELSMKLRKKHIEAYFTEPQ
                     YSTDNAVGCAIIGLNNFLGERV"
     gene            13033..14238
                     /locus_tag="PC471_001193"
     CDS             13033..14238
                     /locus_tag="PC471_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HDF2788331.1"
                     /translation="MKLRALDISEANSYIKRILINDPILSNLKVKGEISNFKVHSSGN
                     VYLSLKDETSKLNCVIFKSNFNRNLKLDNGVKIIANGYISVYERDGAYQLYINEIEIE
                     GIGNLHIEFNRLKEKLNKEGLFDPKYKIPIPKMPNSIGVITSPTGAVIRDIINVIKRR
                     YPKVNIKLYPVTVQGDKSAEEICEAIRFFNHMKNVDTLIVGRGGGSIEELWSFNDEMV
                     AREVFNSQIPIISAVGHETDFTICDFVSDMRAPTPSAAAEIATPSLDDINYKLGNIKS
                     RMSKSLTNQIELDQYRLETVFNKINNYLDSYTIKDKVIQLDKIYDKIIFGIENNLKLE
                     DEKLVKIGALLHNLSPLATMDRGYSITQKNGKVINSIKGLKIKDSIDIVLKDGNLECM
                     IDKIENKEG"
     gene            14240..14455
                     /gene="xseB"
                     /locus_tag="PC471_001194"
     CDS             14240..14455
                     /gene="xseB"
                     /locus_tag="PC471_001194"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HDF2788332.1"
                     /translation="MNLTYEEAYKRLESILSELESKNASLDESLSLYEEGISLYKHCN
                     KLLDDAKLKISKFNQLGIEEDFKIEEE"
     gene            14458..15345
                     /locus_tag="PC471_001195"
     CDS             14458..15345
                     /locus_tag="PC471_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HDF2788333.1"
                     /translation="MEFKQCLKEKASFVEKVLKEYMPKEEGYQKTVIEAMNYSLSAGG
                     KRLRPILTLEACKIVGGNEDEAIPFAIAIEMIHTYSLIHDDLPALDNDDLRRGRPTNH
                     KVYGEAMGILAGDALLNYAFEVMLAGSINKENPEKYLKAINEIAKGAGIYGMIGGQVV
                     DVESENKQIGKEKLDYIHMNKTAAMMVGCMRAGATIGGANSEQMEEITKYAKNIGLSF
                     QIVDDILDIVGDEAKLGKKVGSDIENHKSTYPSLLGLDKSKEIAHNLIDEAKKSIEKL
                     SDDVDFLKGLAEYIIDREY"
     gene            15375..15812
                     /locus_tag="PC471_001196"
     CDS             15375..15812
                     /locus_tag="PC471_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent PAP2 family protein"
                     /protein_id="HDF2788334.1"
                     /translation="MDFFSEIFNNGALGISLIACFLAQFIKIFTGKEKRIELSRILIS
                     GGMPSSHSSFVTSLATVVGIEKGFNSTDFAIITVLALIIMYDAAGVRRAVGKQATILN
                     QMVADIQHGKHIEQKKLKELIGHTPLEVWFGALLGIVTALILM"
     gene            15846..17711
                     /locus_tag="PC471_001197"
     CDS             15846..17711
                     /locus_tag="PC471_001197"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HDF2788335.1"
                     /translation="MYKYLDKVNSPKDIKNMSIEEMDLLAKDIRKFLVKSVSKTGGHL
                     ASNLGVVELTLALHKVFDSPKDKIVWDVGHQSYVHKIVTGRKDCFVSLRQFNGLSGFP
                     KENESPHDIFDTGHSSTSISIATGIACARDIKKENYSVISVIGDGSITGGMALEALNQ
                     LGYIDTNMIVILNDNEMSIDKNVGGMSKYLSSIIRNSTVEKMTDEVDKILNVTQTGEI
                     LSKTAHRFKDKLMYSFSPQDCSFFDSLGIRYYGPIDGHNTKELIDILRKAKHKKGPVL
                     LHVITKKGKGYRFAEEQPDKYHGVSKFDIKTGVTSAKVKSMSISVGEKLVDMANSNEN
                     IVAITAAMPSGTGLNLFESAYPKRYYDVGIAEQHATGFAAGLAKNGMKPYFAVYSSFL
                     QRAYDQVIHDVCITKKPVTFLIDRAGLVGNDGETHHGMFDLSYLNSIPNIVVMAPKDT
                     REMELMMDLSLKLDCPLAIRYPRGSSYYLDKGEYGEIVLGKYEVLDDGQDTVILCIGS
                     MVKHALEAKEILSREGINPTIVNARFLKPIDEGMLKALLKNHKNVVTIEDNIVTGGFG
                     SRINKFIIDNEYNVNILNIAIPEEFVKHGNIDELYDFVGLSPKSIADKIRKLVIE"
     gene            17844..18656
                     /locus_tag="PC471_001198"
     CDS             17844..18656
                     /locus_tag="PC471_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family rRNA
                     (cytidine-2'-O)-methyltransferase"
                     /protein_id="HDF2788336.1"
                     /translation="MKKRIDLLLVEQGYFESRERAKKAIMAGLVFVDNQRCDKAGTEV
                     KEDCSIEVKGNPIPYVSRGGLKLEKAMKNFDLTIDGKVCMDIGASTGGFTDCMLKNGA
                     IKVFSIDVGYGQLAWKLRQDDRVVCMERTNIRNVTIEDTKQFADFASIDVSFISLKLV
                     LPKAKELVRHDGEVVALIKPQFEAGREKVGKKGVVREKSTHIEVIKMISDFSVENGFE
                     ILGLDFSPIKGPEGNIEYLIHLRNGNEGYEFDGETYNNKIVEVVEASHNLDK"
     gene            18676..20355
                     /gene="recN"
                     /locus_tag="PC471_001199"
     CDS             18676..20355
                     /gene="recN"
                     /locus_tag="PC471_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HDF2788337.1"
                     /translation="MILELYMKNCALVEELRLNIDKNLNILTGETGSGKSIIIDALGL
                     CLGDKYDRSFLRKGTDKGLVEAVFFSDNRYLKKILEENDISMEDDNLLVITRLIYSDG
                     KSTARVNGRTVKVSLLKEIASTLIDIHGQHQNQALFNKDTHLKFLDLFGENELEEFKI
                     AYKNVYHKYSEVKKALNCLTENKDEMQIQREIDLLRFQINEIEAANLNKNEYEDLLKQ
                     REVYRNSEKIYNNLNSSYSKLHNGEYNVIDLIGLASKELNDISKYDSVLSEYSDTVER
                     IMYELQDISGEIRNYKDNIDFEPYELEQIELRIDEINNLRRKYGDSIDDIFEYYGKIK
                     DRLDEILNRDERVEQLRSKLMNIEEDLKIKASKLTKARIEVATTLEKILLDELKSLNM
                     KNVMFKVNFEKGPFTLDGVDDIEFMISFNLGEDIKPIYKVASGGEMSRFMLAFKTILA
                     DIDDIDTLVFDEIDTGISGIAAQIVGEKLSDIAKKKQIICITHLPQIAANADTHYCIE
                     KDTSNNRTFTNVSKLNESQRKNEIARLIAGNNITEKTIEHASEIIELAKKC"
     gene            20569..21435
                     /locus_tag="PC471_001200"
     CDS             20569..21435
                     /locus_tag="PC471_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2788338.1"
                     /translation="MNKKDTMHLIFTIVIFILLWYIVVAVSEINKNIESCSNKLDYLS
                     QEIMSTKSDISNTINEEMSKSYITKSIDLKVKKIEKKECVIDVDVQLSKLGKNGKVFF
                     MYKEDSDKWNETEMTKHGELSYACEIKINTGSEYDYKVVTSGAISESSDVQKLTKSDY
                     MPEQPIFNSGIRDNREYFIEIVENSNLFNHESEKSKNKVYDNIRLEKVDIIVNEGKKD
                     TIYKAKLAEGLDDGKTNNSNRNQVNYEVSFPKRDIDDIIYIKAKLTYNNGVEYTKDIT
                     EDITMGLQDLEK"
     gene            complement(21528..22019)
                     /locus_tag="PC471_001201"
     CDS             complement(21528..22019)
                     /locus_tag="PC471_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDF2788339.1"
                     /translation="MADKMAQNITLQFVEEKDLENLENVKLLFTEYSNSLNIDLCFQD
                     FNNELKTLPGKYKKPSGSLILAFVDENLAGCVALKKLEGKICELKRLYVRNQFRGLKI
                     GKILLEEIIEEAKKFGYTHMRLDTLPSMKSAQGLYEKFGFYDIEPYTYNPIEGARYME
                     LKL"
     gene            complement(22080..22520)
                     /locus_tag="PC471_001202"
     CDS             complement(22080..22520)
                     /locus_tag="PC471_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDF2788340.1"
                     /translation="MKLNVRIADINNWHDLVSLSVDKSQLDYIESNALSIAESKFITA
                     WVPVGIYDENSLIGFAMYGRLEDDRVWLDRFMIDSKYQGKGYGKASLDFLVNHLKNEY
                     NCDELYLSIFEDNKMAIKLYKDFGFEFNGELDYGGEKVMVLKSN"
     gene            22681..23739
                     /locus_tag="PC471_001203"
     CDS             22681..23739
                     /locus_tag="PC471_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase S55"
                     /protein_id="HDF2788341.1"
                     /translation="MNKINKNFKNIWIILGAVSIFTMVIIFNKNCNVNKETQIEAFTI
                     KNHDKKFVYPMGNIIGVKANTDGVLVLGYEEEDVDYIGGIQIGDNIVKIDNKRIKNSQ
                     DVSEILNKIKKSKVEVTFERKNKYKTEVIETKKENGKYKLGLWVRDKISGIGTMTFYD
                     PSMEKFKAIGHAIKDSDTNELLKIKQGYIYKPEQLKIVKASNEKVGKIKGDFNDSNLM
                     GNFSNNSELGISGNITENHNKEFNVANKEKQLIEVGRPQDVKIGDAVILFEDKNKNIT
                     SYDIKIESIVYDKGNYRDMVIKVVDDKLLEYTGGIVQGMSGAPIIQNNKIIGAITHVF
                     RDNPKKGYGIFIDEMIKL"
     gene            23881..24705
                     /gene="spo0A"
                     /locus_tag="PC471_001204"
     CDS             23881..24705
                     /gene="spo0A"
                     /locus_tag="PC471_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation transcription factor Spo0A"
                     /protein_id="HDF2788342.1"
                     /translation="MGGFLVEKIKIVLADDNKDFCQVLKEYLSNEDDIDILGIAKDGI
                     EALDLVKKTQPDLLILDVIMPHLDGLGVIEKLNTMDIPKMPKIIVLSAVGQDKITQSA
                     INLGADYYIVKPFDFVVFINRIRELVSNRVTQVEPKPRPVQETQMTRSDFVKNVGNIE
                     TEITNIIHEIGVPAHIKGYLYLREAIKMVIDNVELLGAVTKELYPSIAKKFNTTPSRV
                     ERAIRHAIEVAWSRGKVDTINQLFGYTVHNTKGKPTNSEFIAMIADKLRLEHSMVK"
     gene            25118..26239
                     /locus_tag="PC471_001205"
     CDS             25118..26239
                     /locus_tag="PC471_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2788343.1"
                     /translation="MRVEAPIKVDRKTKKLAKRVESGEIAVINHIDIDEVAANSLVEA
                     KIKLVINAAPSISGRYPNKGPGILTENNILIIDNVGEELFEELKEGETIEVVDGKIYR
                     KGKFLGAGEVLDKYEVSHQIKAAYENLAVELDRFIDNTIDYAKKEKGFILGEVEIPKV
                     KTNYANKHVLIVVRGQDYKEDLSTMLSYIEEVKPVLVGVDGGADALIEFGYTPDVIVG
                     DMDSVSDEALKKASEIVVHAYTDGRAPGLKRVEELGLDAVVFPAPGTSEDIAMLIAYE
                     YKAELIVAVGTHSNMIDFLEKGRKGMASTFLVRLKIGSKLIDAKGVNLLYRSKLKIKY
                     IWALIATALFPVLVVASLSPGVQQFIQLMQLKFRVLLQM"
     gene            26257..27132
                     /locus_tag="PC471_001206"
     CDS             26257..27132
                     /locus_tag="PC471_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper transporter"
                     /protein_id="HDF2788344.1"
                     /translation="MHINMKYYIVTIGAIFIALGIGMLVGFNLNNNQQLSEQQANIIN
                     DLDDKFNILKEKNDKLDSDLASVNRDYEEAVNFINKNVDKILAGSLNGKSIGIISANE
                     NDDYTKNIEDIINKSNGSIAFNIILKENITNPEKLKEMSTKLGTDVKNANDAVNYIID
                     TLKKEDASDILTYLQELDVIKFNFIGDTYLKYDSVVIAGGNDAKDSTKQFEKIEKFVV
                     SKLKSENKYLVEVQNTGVKTSYVELYSKNKVATIDNIDEGIGSISLAILLQQGNIVGN
                     FGRLDTATSLLPSIK"
     gene            27156..27848
                     /locus_tag="PC471_001207"
     CDS             27156..27848
                     /locus_tag="PC471_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HDF2788345.1"
                     /translation="MDSYISIIIPAYNEESRIKYTLESILDIKQINEIIVVDDGSIDN
                     TAKVVSELENEKIKFFKLDKNRGKGYALNYGIKLAMKNADIIGFLDGDLGSSAKEVEK
                     LIVPILNGEADVTIAKFPPAKRKGGLGFVKGLAKNSVFEMTGVELDATLSGQRIFKRE
                     VLEKFDEIPFGYGVEVGMTIDILKYGFKIKEVLVNMTHNETGRNLKGFIHRGKQYYHI
                     KKVLGQKKKEWR"
     gene            27856..28680
                     /locus_tag="PC471_001208"
     CDS             27856..28680
                     /locus_tag="PC471_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HDF2788346.1"
                     /translation="MKDYILYTILLLTGLFGTYAVIPLFKNLLINGNVLRPNYKKDMI
                     PVSMGIVFLPMIVINGIILAFFTTEFKNLAYIFMFMFGMISMFFAGILDDVIGNRDVS
                     GLKGHFKSLFKGSLTTGGFKALFGGFVGLIVSVAISKDIIDIIVNTLIIALSTNLMNL
                     LDLRPGRAIKAYLFIMVIVFLTLTGFVQVLPLLIVPNVLAYFNYDLKARAMMGDTGSN
                     VLGISIGMLISFGYPFNIRLGWLIFVVIMHIFTEKYSLTKIIEKNKLLNFIDKLGR"
     gene            28685..29752
                     /locus_tag="PC471_001209"
     CDS             28685..29752
                     /locus_tag="PC471_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3866 family protein"
                     /protein_id="HDF2788347.1"
                     /translation="MISKRVGIVESIVSQTETLDDIRVNINGEIQKAYNYPKISGTIN
                     IGDEVVLNTTAVELSLGTGGYHFVITNLNNIESTLTEGGHIMKLRYTPLQIKVDSVEE
                     QESIYHDKFANFKGLEGLSVVVGTLHSMLTPFAASFKRNNPNKKLVYIMTDGASLPIY
                     LSKNVDTLKEKKLIDSTITIGNAFGGDYECINIYTALITAKEILKADAVFVSMGPGIA
                     GTGTKYGFTGIEQGSILDAVKKLEGRAIAIPRISFADKRERHQGISHHSMTVFKEIVN
                     VNVDIPITIYDDEKLNFIKEQINLNGLDKKHNIIYIDNNKTKEDLDYFNLKVKSMGRN
                     YEQDEAFFKAASTAAYYLMEV"
     gene            29754..30290
                     /locus_tag="PC471_001210"
     CDS             29754..30290
                     /locus_tag="PC471_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HDF2788348.1"
                     /translation="MVLEEKTISSDRVYTGKVITLKVDTVEIPGQGYQKRELVEVGGA
                     VGIVAITDDNKVVLVKQFRKPIEKPIFEIPAGKLEKNESPKECAERELKEETGYSAKN
                     IKLIHKFFTSAGFSNEIMFVYLATGLTPGENNLDADEFLDVYEIELEEAYNMVLKNDV
                     EDAKTSIGLLLVKDMFKN"
     gene            30358..30963
                     /locus_tag="PC471_001211"
     CDS             30358..30963
                     /locus_tag="PC471_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein M"
                     /protein_id="HDF2788349.1"
                     /translation="MRRTYRKNDFRELNRQIIIIGLLFMMSIVVGSYINKILPGSSNN
                     ILNNINPAVEYYNLNISIKDTVIQNLKSDAIFMGSIALLSLFVVTIPAVLVAFVLKGM
                     SIGYTINSCILALKFKSIKMILIILFKNLIIIPCAIILALISLSYFKEMVYEFKKKSR
                     KNMQFLIKRYILNIIIIIALSLGLQLILNTASIGIIKFLAK"
     gene            30975..31889
                     /locus_tag="PC471_001212"
     CDS             30975..31889
                     /locus_tag="PC471_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDF2788350.1"
                     /translation="MDIIEGYIDYIKDKKKLSENTVASYFMDIKKYMEYINQKSIKLV
                     DIVENDIISYLIKLEKDNVSIATIARMISSIKSFHDYLFLNHICTNNPAKDIKKPKIK
                     KENINILTEEEIERLLNFPKLTTPKLIRDKAIFEVLYGTGIKVSELVEMNIEDIDLDI
                     DYIYCNSCCKNQRVIPLCDITKLYLEKYLKEARPKMAAEGEKSLFVSSLGQRFTRQGL
                     WKVIKKYSNLANIDKNINPTMLRHSFAIHLLNEGANIAVVSKILGNVNLSSLQVYLNH
                     IDKNVRREIKEKHPRNDVDVELQKAEAK"
     gene            31931..33103
                     /locus_tag="PC471_001213"
     CDS             31931..33103
                     /locus_tag="PC471_001213"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HDF2788351.1"
                     /translation="MSRVIWIVIDSVGIGALPDAEKFGDSKDVSTLGNIFKEYPDIQI
                     PNMRNLGIGNIEGIDFFESIKEPIGCFGKCKEMSQGKDTTTGHWEMTGIIVDKPFKTF
                     EHGFSKEIIEEFEKKTGRKVVGNKPASGTVIIDEYGEHQIKTGDVIVYTSADSVFQIA
                     ANEEVIPLEELYNMCKIAREIMMGDNAVARVIARPFIGKKKGEFVRTSNRRDYSLDPF
                     EPTVLDNIKESGLDVLAVGKIEDIFNGKGITDAIHTKSNMDGVDETLNYMKQDNKGLI
                     YSNLVDFDSKYGHRRDPEGYKKALEEFDSRLPEIMANMREDDILIINADHGNDPTYKG
                     TDHTREYIPVMIYGNKIKKGFNLGVKDTFADIGATVADILNVKLPKHGSSFKEDLF"
     gene            33150..33962
                     /locus_tag="PC471_001214"
     CDS             33150..33962
                     /locus_tag="PC471_001214"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HDF2788352.1"
                     /translation="MFEKIAQSSKFINSKSNIKPKIGLILGSGLGDLANDIEDPVTIK
                     YSEIPNFPVSTVAGHAGQLVIGKLEGKEVIAMQGRFHYYEGYSQKEATFPVRVMKELG
                     VEILIVTNAAGGVNKEFKAGDLMLIRDHINFSGSNPLVGKNDDRFGARFPDMSDAYSS
                     KYVDVVKNCAKECNIDVKEGVYMFFSGPNYETPAEVRMAQILGADAVGMSTVPEVIVA
                     SHSNIGVIGISCITNMAAGILNQPLSHEEVIETTQKVKSEFMNLVKTTVKNL"
     gene            34166..35491
                     /locus_tag="PC471_001215"
     CDS             34166..35491
                     /locus_tag="PC471_001215"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HDF2788353.1"
                     /translation="MRMYDIIKKKRDNLELSKKEINYFIENYSKGDIPDYQASALLMS
                     IYLNKMNKQETVHLTEAMMNSGDMINLSEIDGIKVDKHSTGGVGDKTTIALIPLVASC
                     GAPVAKMSGRGLGHTGGTIDKLESIPGFSTEMEISKFIDSVNKSKIAVCGQSAKVAIA
                     DKKIYALRDVTATVDNISLISSSVMSKKLASGADAIVLDVKTGDGAFMKTLEESFELA
                     KSMVDIGTGMNRDTIGIVTDMDEPLGFAVGNSLEVIEAIETLKGNGPKDLVELCEVLG
                     AYMLVLSKVAHDFEDGVEKIRESIKSGTAIQKLKEFIENQGGNKDVVDDYSLFKKSKY
                     KYPVKSTKSGYISKIKAEDVGVSAMILGAGRETKDDILDLSAGIILEKKVGDYVNEGE
                     VLAYMYYNDEQKLSLAKGRFIDSYSIVDSKIEKNKLIYGIVTKEEIKKF"
     gene            35704..36783
                     /gene="mltG"
                     /locus_tag="PC471_001216"
     CDS             35704..36783
                     /gene="mltG"
                     /locus_tag="PC471_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HDF2788354.1"
                     /translation="MNFKENRLKIAVLIIVILIILAGIFVFIQIGPYDKNNKKDVIID
                     VPSGASVGKISDILYENKLIKNELLFKLLVKVSNKAPSIKSGTYLLNQSYSNNDIISL
                     LVSGKIYQDGIKVTIPEGATSKEIIAMLVSKNLGDKATFENLIKKPQEFYDKFSYLKE
                     DGITSLEGFLYPETYYFNSKKQSEEDILSEMLKVFDSKYTDKFKKKQKELNMTLQEVM
                     EMASIIEKEAVLDKDRPIIASVFYNRLKVGMPLQSDATIQYIFEERKKIVTYDDLKID
                     SPYNSYKNKGLPPTPISNPGIKSIEAALYPEKTDYLYFVAKIDGGNNYSTNYQDHLKY
                     VKEYKEARDKQSKDTKATNKENTKK"
     gene            36955..37629
                     /locus_tag="PC471_001217"
     CDS             36955..37629
                     /locus_tag="PC471_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HDF2788355.1"
                     /translation="MSNIVNDLVEDYIRVTLKEKEGFLKDLEIYAEENSVPIIHKEVS
                     DLLKVLLKVQKPKRILEVGCAIGYSSIFFATIIGKDADIITVERNEKMIEKAKENIKL
                     AGFDNNITILEGDAEEKLSQVQGEFDLIFIDAAKGQYKLFFDLVIDKLKDGGLLVSDN
                     ILYKGMVAHDDFVVRRKKTIVKRMRKYLDYICNCDYLSTSLVPIGDGVALSYKESKRG
                     DVDGIK"
     gene            37616..38863
                     /locus_tag="PC471_001218"
     CDS             37616..38863
                     /locus_tag="PC471_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HDF2788356.1"
                     /translation="MELNKVELLAPAGDLERLKIAITYGADAVYIGGEIFGMRSAAKN
                     FSKEDMAEGVAFAHERGKKVFVTVNIIPHNEDFLQLEDYLLELEEIGIDAVIIADPGV
                     LSVIKKVIPNMEIHLSTQANTTNYLSANFWYEHGIKRVVVARELSFDEIYEIRAKTPL
                     DMDIEAFMHGAMCISYSGRCLISNYMTGRDANKGSCAQSCRWQYHLVEEKRPGEYFPI
                     YEDERGTFFFNSKDLCMIEYIPELIKSGITSLKIEGRMKTAYYVATVVRAYRMAIDEF
                     YRDPENWKFNPMWMEELKKGSHRHFTSGFYLNKPTTEDQNYQSASYVRNYDFIGIVRE
                     TEDEDGLIVVEQRNKMCVGDEIEVMGPYKETMFTKIEEMYNEEGEAIESAPHPRQIVK
                     LKLSVKVGKDYMLRKVIEEKVEE"
     gene            38935..40605
                     /locus_tag="PC471_001219"
     CDS             38935..40605
                     /locus_tag="PC471_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HDF2788357.1"
                     /translation="MSKKKTPFLKKVGKRSWCIFTIILIIYSVLIYRLVDIQVLKGDK
                     YKQSVESQSVEKVELNSGRGIIYDRNNKKLTDTSKSQVLIVEKEKLNNNYKILELIKK
                     ATKMNDLDIYKAVQEQLTRPIIQIQTKNIDIDKSMKKELEKNGIMVEEKTMRYAKDGL
                     LSHTIGYIKEDDKSGQSGIEKSMDSVLRNSNEKYISAFKAGDAGNEKSLNILKGSVKT
                     VDNKDKDRHLKTTIDYNIQKKLEQILNKEENPTAAIISEASTGEILAMCSRPNFDQND
                     ISKSLKGKNGEFENRVIKATYPPGSVFKMVVLFSALENGVIDENYTYNCTGKTKVGNT
                     NEILRCNKRDGHGFQNLRQAFSNSCNPAFLDIAMKLGKEKILKSAEKLHLFEKVDIGL
                     DEEKIREAPKNISIRNLAIGQENIEFTPLQINQMTQIIANNGTFKPLYLYKSLVDNNM
                     NTIKTYKSSKKEELISPYVCTQVKEYMKSVSRTGTAKDLKDIEGGCGVKTGTAQSSLN
                     KKAIDHGWITGFYPEERPKYVITVLVEGTQKGNKSATPIFKEICESIK"
     gene            complement(40702..41343)
                     /gene="sigK"
                     /locus_tag="PC471_001220"
     CDS             complement(40702..41343)
                     /gene="sigK"
                     /locus_tag="PC471_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018589741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigK"
                     /protein_id="HDF2788358.1"
                     /translation="MAALKSFEKPLTPEEEIEYLTKFKIENDKSAKDTLIERNMRLVA
                     YIAKKYNNSTEDQDDLISIGTIGLIKAIETYNIDKGTRLATYASRCIENEILMNIRSN
                     KKNKTQVSLQDPIGTDKEGNEISLLDILGTEANYVLDEVELKVQVGKLYEQLNKILTP
                     REREIVQLRYGLTPYGYKTQREIAQKLDISRSYVSRIEKKALKKLEKELVQES"
     gene            41910..42272
                     /locus_tag="PC471_001221"
     CDS             41910..42272
                     /locus_tag="PC471_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2788359.1"
                     /translation="MKIRKSGFISIECIISIAILYVAVYLVSTSLYNCYSFISRNISD
                     REMLSTAKKYIEDEKYRIQNSKYELIEDKIEKNYINGYEINSKIEQILDYYQCYEINI
                     EIKNEFKKLRFNSYVTRK"
     gene            42256..42981
                     /locus_tag="PC471_001222"
     CDS             42256..42981
                     /locus_tag="PC471_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2788360.1"
                     /translation="MLLENKGSILIITLIIFSIISTVCIMCIGLIYTHNNMFELGYKD
                     IQLKEISLSGIEISVSNVLSCVEEAIDVSNNEDEFREYFLGNNMIECIKKIKDTSYSS
                     LEELSLKVENNTIYDRGDFFEFDIESNVREKEFLKAIKVRVKIKNPWIGIDIEDYMDK
                     SNNNKIDDKTFNNYKASENNEILNQFKNESENIRNQEVGGFTEEDEEDEEYEEELKQN
                     IIENIKKNFDEKNLVQVYKYRGV"
     gene            43001..43420
                     /locus_tag="PC471_001223"
     CDS             43001..43420
                     /locus_tag="PC471_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2788361.1"
                     /translation="MTVIELIVSISIILLIVTLSFPKDNLENHKINLFARQLCSDIRY
                     VRRINMFSNYNTYIYYINQGKHEGYVLRKDGKNIKSIMLPKNAKIARSIEIIKFKSDG
                     SPQKASTIEIYNNRLRKTITITPVSGRVLLKEGKYET"
     gene            43410..43934
                     /locus_tag="PC471_001224"
     CDS             43410..43934
                     /locus_tag="PC471_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2788362.1"
                     /translation="MKHKYGYLLLESVVSLSSMVIIILVLYSIFLSTINLKLKVEDKI
                     ELQQQSLEIIKSMEGIISNSMGIMNVSNYEETFKKTTSIKCRYLDENNNEEGISNKEI
                     ILNERRNKLFVNSLNGESSQAGGYEIGDYVDEMYVSITNNGQYVNIKLKLSKRSQKYE
                     TDFKIKVWNFSESI"
BASE COUNT        17901 a         4373 c         8000 g        13742 t
ORIGIN      
        1 aggttaagtt agaataaccc attacctatt tttatttgta agaaaagtgt acatagaatt
       61 agaagtaaaa aatacaacta ataaaactat tgaaaatact agataaataa taaataaaaa
      121 taattataga ttttggcata catattgcta attatagaga taagacgaca aaataattgg
      181 gaggtttaat gatgaaatac aaaacaacag ggacttgtgc tacagaaatt gaatttgaag
      241 taaaagaaaa taaagttaca aatgtaaact ttataggtgg atgtgatgga aatttaaaag
      301 ggctaaaagt attagttgaa ggcatgaata tagaagatgt aatacaaaag ttaaaaggaa
      361 tagagtgtaa aacaaagcca acatcttgcc cagaccaact gtctctagca ttggaaaatt
      421 atatgaatat aaagtaatac ataaaacaat cgattagtta ggagtttaat tatgaaacca
      481 ataataggga ttttaggaaa tttaataata atggaaaatg gtatgtttcc aggtcttgaa
      541 aggtcatatg taaataatga ttatataaat gcagtgctaa aaggtggcgg tagtccagta
      601 ataattcctg taaatacaga taaagaagtt ataaaaaaac agattgaaat ggttgatgga
      661 gtcttaatat ctgggggatg ggatatcaat cctcaactgt atggagaaga aacaagagaa
      721 gaaacgacat ttatatatcc agaagtggat gaatttgatt taattgcaat aagtatagca
      781 ttagaactta aaaagcctat attaggtgta tgtaggggac tacaaatttt aaatgtaagt
      841 ttaggaggaa ctttatatca agataataat cttatagaag gaagttatat aaaacataca
      901 caatcttcta aaagacatgt agctactcat aaagttgatg ttaaagaagg aagtatttta
      961 gagggaattt taggaaaaca attacttaca aatagttatc atcatcaaag tgtcaatcag
     1021 cttggaaaag gattaaaagc catagcatac tcaaaagatg gtataataga agctattgag
     1081 aaagaggatg aaaattttgt agtaggagta cagtggcatc cagaaatgat ggtagattac
     1141 tgtgataaaa tggaaaaact atttaaatat tttataaata tttgttcgaa tttataatat
     1201 accttggggg gattgaacat gaacgaaaag aacaaaatgg gattaataag tattatactt
     1261 ttaggtatta atgcagtagt aggagctgga gtttttctat taccaggaga tgcaatgaaa
     1321 tcatttggtg tagcaagtat atttgtatat atatttgata tgttgttagt tttatctatg
     1381 gcattctgtt ttgctgaggt tgcaggtaaa ttcaataaaa atggtgctgc ctatgtatat
     1441 acaaaagaag cttttggaga tttttgtggt tttgaagtag gtcttatgaa atgggtaata
     1501 ggatgtattt cttggggagc tttaatagta ggttttccta catcattatc agcagtatgg
     1561 gctccagcag gtgaaccaca tatacaaaaa attataattg tagctatgat agttgggctt
     1621 acaattataa acttattagg tgtatctcta tcaaaaattg tacaaaatgt tataactgta
     1681 ggtaaactaa ttccattaat actatttata ggaataggta tattttttat aaaaggagtt
     1741 aattttactt cttcaacaat ggtacctcca ggagctggag caacagagtt tggagcagca
     1801 gcacttttaa tgttttattc atttacagga tttgaatcta tagcagttgc agcagaagat
     1861 atggaaaatc cacaaaaaaa tataccaata gcaattataa gtgtaatagt aattgcatca
     1921 ataatatata tattaaatca agttgtatgt gttggtatac taggagattc gttatctagt
     1981 acatctacac cagtggcaga tgctgcaaga atatgctttg gaaatatggg agctggattg
     2041 gttacctttg gaacattggt ttctgttggt ggaatttgta tgtgtggtgc ttttgtaaat
     2101 ccaagaagtt gtgtagcttt agctgatgat aaaatgttac cgagaatatt tgcaagaaaa
     2161 gataaaaaag gtacaccata tgtagcgata atagctacta tgttaatcac tataccaata
     2221 gctctatctg gaagttttgc tgaattagca gcaataagtg cagttgctag attcatacaa
     2281 tacataccaa catctttatc cgtattagtg tttagaaaga aaagaccaga attagtaggt
     2341 acttttaaaa ctccttttgg agcagtgatt cctctaattg ctgtatgtgt tggaacatgg
     2401 ttgttattcc aagcatctat gcatcaatta attatgggat taggagcttt ggcaattgga
     2461 gtaccattat actttataat gaagtcttat aatagaaaaa tctatggtga aaatttaaag
     2521 aaaatagttt aattatgtta taacaattta ttgagttaaa attagacatt ggcagaaaat
     2581 taaatacaaa taagtatcta caaggctata ttaaaatatt attaattttt tattttaata
     2641 tagccttata ttgttatcaa taaatgaaaa atattgtaac aacagaagaa caatagtatt
     2701 atgtttatag acaaaaaact tattaggagg taaatttatg ccaacaataa cattaaaaaa
     2761 tggtgtagac gtacttatta gagaaggtgt aagagaagat gctcaaagca ttatagattt
     2821 ttataatgaa gtgggtggag aaactcattt tctgtcattt ggaaaggatg aatataaaat
     2881 atctttggag gaacaagaaa atgctataga atcagcaaaa gcatctgata attctgttaa
     2941 actgattgca tttatagatg gtgaaatagt tgggattgca acgattgatt ctaatcaaaa
     3001 agcaaaagga aagcatgttg gagtattagg aattgttgta aaagaaaaat attggggaat
     3061 tggattgggt aaaaggctta tgttagacct tatagaatgg tgtaaatcaa atggaataac
     3121 caagaagata actcttgtca ctaatgaaga aaattataat gctataggat tatacaaaaa
     3181 agttggattt gaagtagaaa gcatactaaa gaaagaatgt tattataatg gtgtttatac
     3241 tgatttaata ggaatgtcac ttttattggg aatataatta gtgtacattt tatctcgaaa
     3301 aaagtatatt aagtatggta taattaaaaa agaatataac ttaatataat tttggaggac
     3361 gaattatgaa aaatctgtgt aaaatatcag atgattattt aaacagcaag cttaagtatt
     3421 taaagttatt atcaaaacaa tatccaagta tatcaaaagc aagtacagaa ataataaatt
     3481 tagaggctat attaaatctt ccaaagggaa cagagcattt tataacagat gtacatggtg
     3541 aatatgaacc atttgtacat gtattaaaaa atggttctgg tgtaataaag agaaaaatag
     3601 aagaattgtt ttcaaataca ataagagaca gcgaaaaaaa aatgttagca actcttgtat
     3661 attatcctga acaaaagtta gacctaataa ttaagcaaga ggaaaatata gatgattttt
     3721 atagaataaa tatttataga cttatagaac tttgtaaata tgcttctagc aaatatacta
     3781 ggtctaaagt tagaaaactg ttaccagaaa attttaaata cataatagaa gaacttttgc
     3841 atgagcatgt taaaagtgaa cataaagaag agtattataa aagtatagtt gaaacaatag
     3901 ttgacatagg aatagctaag gagtttataa ttgctatttc tacagttata caaaaattgg
     3961 ttgtagatag acttcatgta ataggagata tttatgatag aggtccaaga ccagatataa
     4021 ttgtcgataa gcttattgaa catcattgtg tagatattca atggggaaat catgatatat
     4081 tgtggatggg tgcagcctca ggtgaaaaaa cttgtatagc aaatgcactg aggatatcag
     4141 ctagatatgc gaatttagac attgtagaag acatatatgg aataaatcta ttgcctttag
     4201 ccacttttgc tatagaaatg tataaagatg acccttgtaa agaatttatt ccaaaggtta
     4261 atgaccaaag tgtaacaaca acagaaaagt ctttaatggc aaaaatgcac aaagcaataa
     4321 gtataattca atttaagctt gaaggtgagg ttatcagacg aagacctgaa tttgaaatgg
     4381 aacatagact cttgcttaat atgataaact atgatgaagg tacgattaat ttaaaaggta
     4441 aaacatataa attaaaagat acttatttac ctacaataga caaaaaagac ccatataaat
     4501 taactatgga agaaagaaat gttattgata aattagtatc ttcatttaga ggtagtgaaa
     4561 aactacaaaa acatgtttca tttttgtttt ctaaaggaag tatatattta aaggctaact
     4621 caaatttact gattcatgga tgtgtaccgt taaatgaaga tggaagcttt atgtctatga
     4681 atataatggg aaaagagtat aaaggtaagg ctcttatgga taagatggag tctctagcaa
     4741 gagaaggatt tttctttaaa gataaggcag aagaaaaact gtatggcatg gatattatgt
     4801 ggtatttatg gactgggaaa tgttcatcat tatttggtaa agatgatatg actacatttg
     4861 aaagatactt tatagctgaa aaagagactc ataaagaaaa taagaaccca tattttaaac
     4921 ttagagaaaa tgagatggct tgtaaaagat tatttgaaga atttgattta gaacttgatg
     4981 aatctcatat aataaatggt catgttcctg tggagagtaa aaatggagag agcccaataa
     5041 aagctaatgg taaaattctc gttatagatg gtggtttttc tagggcttac caaaaaacta
     5101 ctggtatagc tggatacact ttgatataca attctagaac tttgcaatta gtatcgcatg
     5161 aaccatttaa ttctgctgaa gaagccattg caaatgaaag tgatatctta tcgacaactg
     5221 ttgtagtaga gcataaggca aagagaaaaa tggtgagaga tactgatgaa ggaataaaga
     5281 tacaagagga aatagaagat ttaaaactac ttttaatggc ttataagaaa gggctaataa
     5341 aagaaatgta gatgttatag aggcacttca tgattgaagt gtctttttgt taatacttat
     5401 taaatatatt ataaagatta ataatagtag tttatattat acttactaag tgaatccaag
     5461 ataaatgggt tcactttttt tatttataaa cattattaaa aaaatataga tgttttggtt
     5521 atattccttg tcctttataa atagaattat ataaacaaag gaggaggaca agagatgaat
     5581 aaactttctg atgaaataat aaactctctt tctacaacta taagagaaaa ggtagaaaaa
     5641 gtctcaaaca ataatctgaa catagaagaa attaggttac gctcacaaaa accattgata
     5701 ctaaatgcaa actctaaaga ttatttttac aatcaaaaaa ctatgacact tgatttaaat
     5761 caacaaaact catatgtagt aacaagagaa gatgtagagc aaacttttca aataatatgt
     5821 aaatattcaa tacattcatt tatggatgac ataaaaaaag gattcataac tttgagagga
     5881 ggtcatagag tagggttagt tggaaaagca atagtagaag atggacaagt caaaaatata
     5941 aagcatatat catctttaaa tataagggta tcaagggaaa ttattggatg ctcggataag
     6001 attttaagtc atatcataaa gggaaaaaat cagattaata atactttgat aatatcacca
     6061 cctcaatgtg gaaaaacaac tttaataaga gatatagtaa gaaatttaag taatggtaat
     6121 gaagattatg gatttaaagg tttaaaagtg gctttagtag atgagcgtaa tgagatagca
     6181 ggagcttatc taggtgttcc acaaatggat gtgggaataa ggactgatat aatagaaact
     6241 tgcccaaaag acttgggtat tacaatgctt ttaaggtcta tgtcaccaaa tgttattgtg
     6301 acagatgaaa ttggaagtga aaaagaaata aaggcattat acacagcatt aaatggaggt
     6361 ataggattaa taactactgt gcatggagat tcaatagagg atatacaaaa tagaaaagaa
     6421 ttaaatagac ttctggataa agagttattt aaaaaggtta taattctttc tgcaaaaaga
     6481 ggagcaggaa caattgaaaa aatttatgat ttagaagaaa agagatggta ttttgcaaat
     6541 taaaataatt attatagctt tcttgatagg aagtagttat ttgataggtg aacaaatata
     6601 caaaacttat actcgtagac ataaacaatt aaacgattta ataagagttt tagaaatttt
     6661 gaggatggat ttatcttttg gactttatac attagaagaa atctttaata ggataggtgg
     6721 aaataaagag ttttgttttt ggaaattttt ctatcaaata tcagaaggac ttcacaatga
     6781 acaaagtaaa actttggaaa taataatatc agaaaatatt gatgtcttaa gtaaagaaac
     6841 ttatctaggc aataaagaaa ttgaggaact gaaaaatcta atacttactt tagggaaaag
     6901 cgatatagag tcacaacaaa gaatgataga tttatctata gaaaatttga aaaaacaaac
     6961 ctatgagaca aaggaagata taaacaaaaa aggcgtattg tataaaaaat tagtgacatt
     7021 cataggtata ggtatatgta ttatattgat ttaatatggg gaggtttatt atggaaatat
     7081 cattgatatt aaaggttgca ggtgtcggta ttctaatatc agtattgaat atgatattgg
     7141 aaaaaacaga tagaaaagat tgggcaggtc ttactacatt agcaggtgta ataatcgtat
     7201 tgggtatggt aataacagaa ataagtgact tgtttaatac tgtaagaaca atgtttcaac
     7261 tttactaaag aggagggagt aatttggaaa taatgcaatt aataggaatt gcaatcattt
     7321 caactacact ctgtttagta attaaaaaag atagaccaga gatagcaaat ttcatagcta
     7381 taataactgg agtaataata ctattgtcag ttatgtttaa gctaaatttt atagtagata
     7441 gtatacaaga tttggctaac aaggctaaca tcccaactat gtatatatca ttaataataa
     7501 aattaattgg gatagcatac ttaatggaat ttgcgataca actttgtaaa gattgtgggg
     7561 aaggaaacat tgcttcaaaa ctagaatttg gaggtaaaat aatagttatg tcaatgtctt
     7621 tccctatact tttgtccatt gtagaaatgg tggtgaatat aattccatag atgtgtatct
     7681 tggaatgaat ataagtatat tagagagctt tcgttgtatt gaggtttata gagtgaagag
     7741 aggcatggtt gcttatgaaa aaaagatttt taccaatgat tataggtttt ttatttactt
     7801 tcttttttgt aaatatcttt gctattatga tatttgctaa tgaagtacca agtagtgaag
     7861 ataaagaata tggtgaaaca aaaaatagca tagataagta tatagatggt caattagaca
     7921 agctagatat aaatgagatt caagactata taaataaaga aatagttatt aatgatgtta
     7981 atttaaaatc atttgtaaaa gatttaatta gtggagaaaa aaatatctta gatttattca
     8041 ataaagatgg attgaagata ttgatgtttg atgaatttaa agcaagttta aaggtagtag
     8101 cagtaatttt ggtactggct ctgttatcat ctattttaaa gagtctggaa aactcttttt
     8161 catcaggtgc agtaagtcaa atagcaacat atataatatt tatcacaatg gtatcactta
     8221 cacttattgg ttttaaagat gtattacaga tttgctatga tgcaatagac catactgtag
     8281 gtcttatgca agttataatg ccaatattaa taacattttt acttttaatt ggattcccaa
     8341 taacgtcgac tactttgaat cctatcttca taggaggagt aacatttata aatgtatttt
     8401 ttaaaaattt tctatttgta tctattactg ttgcatttgg aattttaata ataaacaatt
     8461 tatcaaaaaa tataaggcta aagagatttt tctcctttgt aaaacaaata aattatgttt
     8521 ctattggagc tatgtttact gtttacttag gtcttgtatc catacaagga ttgtatgtaa
     8581 ccagttttga taaattcagt gtaaagactg ctaaatttgc tataggtaat tttattcctg
     8641 ttgtaggtgg atttgtatct gactctgtgg atattttatt atcttcatct cagcttataa
     8701 aaaatatatt tggaggaata ggacttatct tacttgttgg aatttgctta cttccagtaa
     8761 taaaaatttt atcagtaata gtagtgtaca aattagcagc aataattgta gagcctgttg
     8821 gagaggatgg aatatctaat tttttaaatg aagtggcaaa tttaatgata ataatgctag
     8881 catcagtaat tgctataaca gtaatgttct ttgtaactgt agctatcttg acctcgataa
     8941 gtgtcgtaag tcaagggtaa tttatatatc agtgaaaaat aattacattt aatctaatgt
     9001 ttttagagag gaggatgcta tgttagaggg cataaaggca tggatagtta gtgttttgat
     9061 aggagcattt atagtaaata tagtagatat gattttacca agttctaaga taaaacctta
     9121 tgtcaaccta gttttaaact ttatgtttgt atttattgta ataactccag tagtaggatt
     9181 tttttcaaaa gatatgagtt tagaagatag aatattaaaa tctatgggaa actacaacaa
     9241 gcagtatgta gatagtacaa atgctttggc aaaagagact ggaaataata gtctatccaa
     9301 gggatatgag gatgggttaa aagaagtact taaattaaaa cttgatgaat atggttatga
     9361 tttagaagat atagagctta atggggcaaa tataaataat ataaaaataa aagaaaagaa
     9421 taatagtgca aaaagtaata acagttccaa tgaagaaaat aaaaataata gtactaaaag
     9481 agaggaagaa aacataaact ctaatgacaa agaaaattct aaacaagtct tcaaaaaagg
     9541 tacagagtat gggttgaacc taaatgaaga aaaattaaaa aacgacttaa taaaggtgct
     9601 ggatgtttct atagaagata tacaaattga taaataggag ttgggagaat gttgaaaaac
     9661 ttaaatgaca aagataagag aaaggtatat tcactaataa ctatagcagg aatatgtgtg
     9721 gtttcattag tattattatc ttgttttcct agtagcaaaa gcgataaaca agttggaaaa
     9781 acagagacta ataaaaatgc tgaaaagcaa gtcactaaag aacaagaaaa agatgattta
     9841 gagtcaaaat taacagcaat actatctaaa attgatggtg caggagacgt tgatgtaatg
     9901 gttacatttg aatcaagtga agaaatacaa cctgctttta attcaaataa tacaacagaa
     9961 acaacagagg aaaaagatgc acaaggtgga gaaagaacag ttacgacttc tagtgaaaat
    10021 aaaacaatga taacttcaaa ttcaagtgac cctgtagtta taaaaactac agagccaaag
    10081 ataaagggag tgatagtggt agcaagtgga gcaagtgacc caagtgttaa agagacactt
    10141 tatagtgcag tacaaacatc attacaagta gctggtcatc aagtagaaat atattctaaa
    10201 taaatacata taaccggggg gataaatgaa tgaagtttaa ttataaggga agaggatttg
    10261 taataataac tttaactgca atgttagtgg tagtaggaac agtgaattat caactaagta
    10321 agaaatcttt attagaaaca tcaaaagagt ttaaggcata tgaagaagca cagttgcaaa
    10381 aaaatactga tgagagtgat agtagtaaga cagaggattc tagtaatgta gataaacaag
    10441 atggtaagga aagtgctgat attgatatag ttgatagcaa agctagtaaa gtaaaagaaa
    10501 aggcaactga gacaagtaag gaaataaaag ctcaattatc atcagagaaa aatatgaaaa
    10561 aggcatctta tattttagat atgaaaatga atagagaaaa acaaagaaat gagttagttc
    10621 aagatttaaa tgaaatgata aataatccat ctacaacaga agaatctaga aaagaagctt
    10681 ctaatatgaa attaaatata gttaaaattc aagaaaaaga acttcaaata gaaaaccttt
    10741 tgagtacaaa agggtatgaa gaagcgttag tttatataag cgataacaaa gttaatgtag
    10801 ttgtaaatga agctaaatta gaaaagaaag atgcagcaaa aatatttgat ttagttgcag
    10861 aacaagccaa tgtgaagtat gaaaatatta aacttacaaa taataatagt aataagtaaa
    10921 gtataaacct cagaattaac tgaggttatt ttcttgcaaa gtagacttgt atttggtatt
    10981 attatactta taaaaatagt tccttaaaat caggaggtaa gttttaatat ggaagataat
    11041 aaatttggac aagtaaaaat atctaatgat gtaatagcta caatagctgg tttagcagca
    11101 ttagaagttg aaggcataga aactacagca acattaacag ataagttgtt aaaaaataat
    11161 ggagtaaaaa tacaaatcga agaagaggat gttaatttag atgtaatggt tacgataaag
    11221 tatggaatgt caataccaga tacagctttt aaagttcaag aaaatgtaaa aaatactgtt
    11281 gagacaatga caggactgaa ggtttctcaa gtaaatatac acattcaagg aatcagcttt
    11341 aagaaggata aagttgataa agaagaagca aaagcaacta agaaaaatta atattagccc
    11401 tctgaaaagt cagagggttt tttgctggta attgatttta tatggataat taaacttaaa
    11461 ggagaatttt gattgatgaa aaaagatagg gcacaaaaga gtacaacaag agaatatata
    11521 atgaaactta tataccaaat aaatattaat aaagaagatt ttgaaacttt agaagataaa
    11581 gtagataatt ttttaaaaga taattcagaa catattataa atagatataa agaacttgca
    11641 cttcaatact caaaaaatac aaatttaaag ttagaagata ctgaaattga agatgttata
    11701 gacaaaaaat atataaatac agtatgcaaa gcattgaaag aaaatcatga caaaatagat
    11761 gagttaataa ataagcatgc taaaaactgg acagtagata gaatgccaaa ggtagatgtt
    11821 tctatactta gattgtctgt gtgtgaaata ctttatttag acactcctaa taaagtatca
    11881 ataaatgaag cagtagaact tgcaaaaata tattgtgatg ataagtctcc taaatttata
    11941 aatggtatat tgggaagtgt tgttgatgaa attgggaaat aatataataa ttggaattga
    12001 tactagctgc tatactactt ctatagcagc tatctcttta gataagaaag ttatattcaa
    12061 tgaaaaaata atgttagaag taagggataa ttcaaaaggt ttaaggcaaa gtgaagctgt
    12121 atttcaacat atcaataatt tagggattct tagtgatagg ataaaatctt ttaaagataa
    12181 gtttaatgta gaaggagttt gctcatcaaa aaaaccgaga cctgttgaaa actcatatat
    12241 gcctgtattc aatgtaggtc ataattttgg aaaattattg tctagtatat atggttgtag
    12301 gttttatgag actactcatc aagagaatca tattgaagca agtttattaa atagtaaatt
    12361 aaaaaataat aataaattta tttctgttca tatgtctggt ggtactacag agatactttt
    12421 aacaagcaaa caagatagtc atcacaatgt atgtgatact aatttaggta aaattgccaa
    12481 aataagtatt aaaaaagatg ataaaagtaa gttatacaat aattttggat ataatataga
    12541 tataattgga ggaagtaaag atattagctt tggtcaactt atagatagag taggaattaa
    12601 gcttggatat aaatttcctt caggaaagta tttggatgaa aatgctttaa attgtaatct
    12661 taaaatagaa agtggtctga aaacttctgt aagagatggg tatatgaatt tatcaggatt
    12721 agaaaatcag gtaaataaga ttataaatga taatggagat aatactaatc aaaaagaata
    12781 tatatctaaa ttggtattag attcagtagt tagaaatatg tttaaatctt tagtgtattt
    12841 atgtgaaact tataatgtaa atgaagtagt atttgctggt ggagtttctg caagtaagta
    12901 tattcttaga gaattaagca tgaagttaag aaaaaagcat atagaagctt attttacaga
    12961 accacaatat tcaactgata atgctgtcgg gtgtgctata ataggattga ataacttttt
    13021 aggagaaaga gtatgaaact aagagcttta gatataagtg aagcaaactc atatattaaa
    13081 agaattttaa taaacgaccc aatactttct aatcttaaag ttaaaggaga aatttctaat
    13141 tttaaggttc atagtagtgg gaatgtatat ttatcactta aagatgaaac atcaaagtta
    13201 aactgtgtaa tttttaaaag taattttaat cgcaatttaa aattagataa tggtgtaaaa
    13261 attatagcta atggatatat atcagtctat gaaagagatg gtgcgtatca attatacatt
    13321 aatgaaattg aaatagaggg aataggaaat ttacacatag agtttaatag attaaaggaa
    13381 aaacttaata aagaaggatt atttgaccct aaatacaaga tacctatacc taaaatgcca
    13441 aactcaattg gcgttataac atctccaaca ggtgctgtta taagagatat aataaatgta
    13501 ataaaaagaa gatatccgaa agttaatata aagctttatc cagtaacggt tcaaggagat
    13561 aaatcagctg aagaaatctg tgaagcaatt agatttttca atcatatgaa gaatgttgat
    13621 acacttatag ttggtagagg tgggggttcg atagaagaac tttggtcttt taatgatgaa
    13681 atggtagcaa gagaggtttt taattctcaa attccgataa tatcagcagt tgggcatgaa
    13741 acggatttta ctatttgtga ctttgtgtct gatatgagag ccccaacgcc atcagcagca
    13801 gctgagattg ctactccatc attggatgat ataaactata aacttggaaa cataaaaagt
    13861 aggatgagta agtctcttac aaatcaaata gagctagacc aatataggtt agaaacagta
    13921 tttaataaga taaataacta tttagattcg tacacaataa aagataaagt tatacaatta
    13981 gacaaaatat atgataaaat aatttttgga atagaaaata atttaaaatt agaagatgaa
    14041 aaattagtaa aaattggagc attactccat aatttaagtc cattagctac tatggataga
    14101 ggttatagta taactcaaaa aaatggaaaa gtaataaata gtataaaagg attaaaaata
    14161 aaggatagta tagatatagt attaaaagat ggaaatttag aatgtatgat agataaaatt
    14221 gaaaataaag agggatagta tgaatttaac ctacgaagaa gcttataaaa gattagaaag
    14281 tattttaagt gaattagaat ctaaaaatgc tagcttggat gaatctttaa gcctatatga
    14341 agaaggtata agcctttata aacattgtaa taagctttta gatgatgcta aattgaaaat
    14401 aagtaaattt aatcaattgg gaatagaaga agactttaaa atagaggagg aataaaattg
    14461 gaatttaaac aatgtctaaa agaaaaagca agttttgtag aaaaagtact taaagaatat
    14521 atgcctaaag aagaagggta tcaaaaaact gttattgaag ctatgaacta tagcttaagt
    14581 gctggaggta aaagattaag accaatactt acattggagg cttgtaaaat agttggaggt
    14641 aatgaagatg aagctattcc ttttgctatt gcaattgaaa tgatacatac atattcacta
    14701 atacatgatg atttgccagc tcttgacaat gatgatttaa gaaggggaag accaacaaat
    14761 cataaagtat atggagaagc tatggggatt ttagcaggag atgctttgtt aaactatgct
    14821 tttgaagtaa tgcttgcagg ctcaatcaat aaagaaaatc cagaaaagta tctaaaagct
    14881 ataaatgaaa ttgccaaagg tgctggtata tatggaatga taggtggtca agtagtagat
    14941 gtagaaagcg aaaataaaca aattggaaaa gaaaaattag attatataca tatgaataaa
    15001 actgctgcaa tgatggtagg ctgtatgaga gcaggagcta ctataggtgg agcaaattct
    15061 gaacaaatgg aagaaataac taaatatgca aaaaatatcg gattatcatt ccagatagta
    15121 gatgatatac tagacatagt aggagatgaa gctaagttag gtaaaaaggt tggaagtgat
    15181 atagaaaatc ataagtcaac atacccatct cttttaggtt tagataaatc taaagaaatt
    15241 gcacataatt tgattgatga agcaaagaaa agtatagaaa aattatctga tgatgtagat
    15301 tttttaaagg gattggctga atatataata gatagagaat actaaaatat acagctaaaa
    15361 tcgaggaggg aaaaatggat tttttttcgg agatttttaa caatggggct ttgggaataa
    15421 gtctgattgc ttgtttctta gcacaattta taaaaatatt tactggtaag gaaaaaagaa
    15481 ttgagttatc aagaatactt atttcaggag gtatgcctag ttcccatagt tcttttgtta
    15541 caagtttagc tacagtggta ggtattgaaa aagggtttaa ttctactgat tttgctataa
    15601 ttaccgtgct tgccttaatt ataatgtatg atgcagcagg tgttagacgg gctgttggta
    15661 aacaagctac tatattaaat caaatggtgg cagatataca gcatggaaag catatagagc
    15721 agaagaaatt aaaagaatta ataggtcata caccactaga agtttggttt ggagctttac
    15781 ttggaatagt aacagcttta atattgatgt aaattgaaaa tttacagaat aaaggtgagg
    15841 ataatatgta taaatattta gataaagtaa attctccaaa ggatataaaa aatatgagta
    15901 tagaagaaat ggatttactt gcaaaggata taagaaagtt cttagtaaaa tctgtttcaa
    15961 agacaggagg acacttagct tctaatctag gagtggttga gttaacatta gcacttcata
    16021 aagtatttga tagccctaaa gataagattg tatgggatgt agggcatcaa tcttatgttc
    16081 ataaaatagt tactggaaga aaagattgtt ttgtatcttt gagacagttt aatggactaa
    16141 gtggttttcc aaaggagaat gaaagtcctc atgatatttt tgatacagga catagcagta
    16201 catctatttc tatagctaca ggtattgcct gtgctaggga tataaaaaaa gaaaactata
    16261 gtgttatatc tgtgatagga gatggttcta taacaggagg tatggcactt gaagctttaa
    16321 atcaattagg atatatagat accaatatga tagtaatcct taatgataat gagatgtcca
    16381 tagataaaaa tgttggagga atgtctaaat atctgtcaag tattataaga aactcaacag
    16441 ttgagaaaat gacagatgaa gttgataaaa ttttaaatgt tacacaaaca ggtgaaattt
    16501 tatctaagac tgcacataga tttaaagata aactaatgta tagcttttct cctcaggact
    16561 gttcattttt tgattcatta ggtataagat attatggacc tatagatggc cataatacta
    16621 aagaactaat agatatattg agaaaagcta aacacaaaaa agggcctgtt cttttacatg
    16681 taattacgaa aaaaggaaaa gggtacagat ttgcagaaga acaacctgat aaatatcatg
    16741 gagtatcaaa gtttgatata aagacaggtg ttacatctgc aaaagttaag tctatgtcaa
    16801 ttagtgttgg agaaaaactt gttgatatgg ctaatagtaa tgaaaatata gtagcaataa
    16861 cagctgcaat gccatctggt acagggttaa acctgtttga aagtgcatat ccaaaaagat
    16921 attatgatgt tggtatagct gaacaacatg caacaggatt tgcagcaggt cttgcaaaaa
    16981 atggtatgaa accttacttt gctgtgtact catcattttt gcaaagggca tatgaccaag
    17041 ttattcatga tgtatgcata acaaaaaagc cagttacttt tcttatagat agagctggat
    17101 tagttggaaa tgatggagaa actcatcatg gtatgtttga tttaagttac ttaaattcta
    17161 ttccaaatat tgtagtaatg gctccaaaag acacaagaga gatggagctt atgatggatt
    17221 tgtcattaaa attagattgt ccactagcaa ttagataccc aagaggaagt agttattacc
    17281 tagataaggg agagtatgga gagattgtac tgggtaaata tgaggtatta gatgacggtc
    17341 aagatacagt catactttgt attggaagta tggtaaaaca tgctttagag gctaaagaaa
    17401 tattatcaag agaaggaata aatccgacaa tagttaatgc tagattctta aagccaattg
    17461 atgaaggtat gttaaaagca ttattaaaaa atcataaaaa tgtagttact atagaggata
    17521 atattgtaac tggaggattt ggaagtagaa taaataagtt tattatagat aatgaatata
    17581 acgtaaatat acttaatata gcaataccag aagaatttgt aaaacatggc aatatagatg
    17641 aattatatga ttttgtagga ttatcgccaa aaagtattgc tgataaaata aggaaattag
    17701 ttattgagta aaaatctaat gtagatattg taaattaaaa tttatataaa atatagaatt
    17761 tatcaattag aataaatatc aagtgtgtta cttcaagcta ctttagttat cattaaagta
    17821 gcttttaaca gaaaggtaat tgtatgaaaa aaagaataga tttattatta gtagaacaag
    17881 ggtactttga aagtagagaa agagccaaaa aagcaataat ggctggattg gtttttgttg
    17941 ataatcaaag gtgtgataag gctggtacag aagtaaaaga agattgtagt atagaagtaa
    18001 aaggaaatcc aataccatat gtcagtagag gtggtttaaa gcttgaaaaa gcaatgaaaa
    18061 actttgattt gactattgat ggaaaggtat gtatggatat aggggcatcc acaggtggtt
    18121 ttacagattg tatgctcaaa aatggagcaa taaaagtatt ttctatagat gttggttatg
    18181 gtcaacttgc atggaaattg agacaagatg atagggttgt gtgtatggaa agaaccaata
    18241 taagaaatgt gacaatagaa gatacaaagc agtttgctga ttttgcgtca attgatgtgt
    18301 cttttatatc gttaaagtta gtacttccaa aggcaaagga gttagtacgt catgatgggg
    18361 aagttgtcgc tttaattaaa cctcaatttg aagcaggaag agaaaaagta ggtaaaaaag
    18421 gagtagttag agaaaaatct acacatatag aagttataaa aatgatttct gatttctcag
    18481 tcgaaaatgg atttgagatt ttagggttag atttttctcc tattaaaggt cctgaaggta
    18541 atatagaata tctaattcat ttgagaaatg gaaatgaagg atatgaattt gatggtgaga
    18601 catacaataa caaaatagta gaagttgttg aagcttctca taatttagac aaataggtta
    18661 aaagggggct tagatttgat cctcgaatta tatatgaaaa attgtgcctt agttgaagaa
    18721 ctaaggttga atattgataa aaacttaaat atacttacag gtgaaactgg ttcaggaaaa
    18781 tctataataa tagatgcact tggactttgt ttaggtgata aatatgatag gtctttttta
    18841 agaaaaggaa cagataaggg acttgttgag gctgtatttt tttcagacaa tagatatctt
    18901 aaaaaaatat tagaagaaaa tgatataagt atggaagatg ataatttatt ggtaataact
    18961 aggttgatat actcagatgg taagagtact gctagagtaa atggaaggac tgttaaagtg
    19021 tctcttctta aagaaattgc atctacactt atagatatac atggtcaaca tcaaaatcaa
    19081 gctttgttta ataaagatac tcatttaaaa tttctagacc tttttggaga aaatgaattg
    19141 gaagagttta aaattgctta taaaaatgtg taccataagt atagtgaagt aaaaaaagct
    19201 ttaaattgct tgacagaaaa taaagatgag atgcaaattc aacgagaaat agatttatta
    19261 agatttcaaa taaatgaaat tgaagctgca aatctaaata agaatgaata tgaagattta
    19321 ttaaaacaaa gagaagttta tagaaatagt gaaaagatat ataataatct aaattcgagt
    19381 tatagcaagt tacataatgg tgagtataat gttattgacc taataggtct tgcatctaag
    19441 gaacttaatg acatatctaa atatgacagt gttttaagtg aatatagtga tactgtagaa
    19501 agaatcatgt atgagttaca agacatatct ggtgagataa gaaattacaa agataatata
    19561 gactttgaac cttatgagct agagcagatt gagcttagaa tcgacgagat taataactta
    19621 agaagaaaat atggagacag tatagatgat atatttgagt attatggaaa aattaaagat
    19681 agattagatg aaatattaaa cagagatgaa agagttgaac agttaagaag caaactgatg
    19741 aatatagaag aagatttaaa aataaaagcc agtaaattga ctaaagcaag aatagaagta
    19801 gcaactactt tagaaaaaat acttcttgat gaattaaaaa gcttaaatat gaaaaatgta
    19861 atgttcaagg ttaattttga aaaaggtcca tttactttag atggagtaga tgatattgaa
    19921 tttatgatat cttttaactt aggagaagat attaagccta tatataaagt tgcatcaggt
    19981 ggtgagatgt ccagatttat gttagcgttt aaaaccatat tggctgatat agatgatatt
    20041 gatacattag tgtttgacga gatagatact ggaataagtg ggatagcagc acagatagtt
    20101 ggtgagaaat tgagtgatat agcaaagaaa aaacagataa tatgtataac tcatttacct
    20161 caaattgctg caaatgcaga cactcattat tgtatagaga aagatacttc aaataatagg
    20221 acgtttacca atgttagtaa gttaaatgaa tctcaacgaa aaaatgaaat tgccagactt
    20281 atagcaggta acaatataac agaaaaaact attgaacatg caagtgaaat tattgagttg
    20341 gctaaaaaat gttagtgaaa aacaagcatt ttaataggtg taaagaattt ttatagaaat
    20401 gctttacatc tattttttat gatgaaatta ttttaaaata tctagtgcct atattgaaga
    20461 aaagtagatt taaaaataga agaaaatttg tataaatttg taactttgaa atttctaaat
    20521 gcttaagtat aatattattt attaaggtag tagtgagggg gacataaaat gaataaaaaa
    20581 gatactatgc acttaatttt tacaattgta atttttattt tattatggta tattgtggta
    20641 gcagtaagtg agattaataa aaatattgaa agttgtagta ataaattaga ttatttgtca
    20701 caagagatta tgagtactaa atcggatatt agtaatacta taaatgaaga aatgtcaaaa
    20761 agttatataa caaagagtat tgacttaaaa gtaaaaaaaa tcgagaaaaa agagtgtgtt
    20821 attgatgtag atgttcaatt aagtaaactt ggaaaaaatg gaaaagtatt ttttatgtat
    20881 aaagaagata gtgataaatg gaatgaaact gagatgacaa aacatggaga attatcatat
    20941 gcatgtgaaa ttaagataaa tacaggaagt gaatatgact ataaagtagt tacaagtgga
    21001 gctatatctg aaagttctga tgtgcaaaaa ttgactaaat ctgattatat gcctgaacaa
    21061 cccatattta attctgggat aagagataat agagaatact ttattgaaat agtagaaaat
    21121 agtaatcttt tcaatcatga gagtgaaaag tcaaaaaata aagtttatga taatattaga
    21181 ttagaaaaag tagatattat tgtaaatgaa ggaaaaaaag acactatata caaagccaaa
    21241 ttagctgaag gcttagatga tggcaaaact aataattcaa atagaaatca agtgaattat
    21301 gaagtaagtt ttcctaaaag ggatattgat gacataattt atattaaagc taaacttaca
    21361 tacaataatg gagtagaata tacaaaggat ataactgaag atataactat gggattacaa
    21421 gatttagaaa aataaataca tatttaaata aatattcaaa tagatttgtt caataaaata
    21481 gagtataaat aaaactgtag tagttttact tatactctat taaaaaatta taattttaat
    21541 tccatatatc tagccccttc aattggatta taagtataag gttctatatc ataaaaacca
    21601 aacttttcat ataatccttg tgcacttttc atgcttggaa gagtatctaa tctcatgtgt
    21661 gtatatccaa attttttagc ttcttcaatg atttcttcca atagtatttt tcctattttt
    21721 aatcctctaa attgatttct tacatataat ctttttaatt cacatatttt accttcaagt
    21781 ttttttaaag caacacaacc tgccaaattt tcatcaacaa atgccaatat caatgagcct
    21841 gaaggttttt tatattttcc tggaagtgtt tttaattcat tattaaagtc ttgaaaacat
    21901 aagtctatat ttagagaatt tgaatattca gtaaatagaa gttttacgtt ttcaaggttt
    21961 tctaaatctt tttcttcaac aaattgtaaa gttatatttt gagccatttt atccgccatt
    22021 atataaacat gtttatataa tgacttcacc tccttgatat atgtatattt ttcttaatat
    22081 caattagatt ttaataccat tactttttca ccaccatagt caagctctcc attaaactca
    22141 aatccaaaat ctttataaag cttaatagcc attttattgt cttcgaatat actaagatat
    22201 agttcatcac agttatattc atttttaaga tgatttacaa gaaaatctaa tgatgctttt
    22261 ccatatcctt taccttgata ttttgaatca atcataaatc tatcaagcca aactctatca
    22321 tcttctaaac gaccatacat agcaaatcct attaaagaat tttcatcata tattccaaca
    22381 ggtacccaag ctgttataaa tttagattca gcaatagaaa gtgcattgct ttctatataa
    22441 tctaactgac ttttatctac agacaaacta actaaatcat gccaattatt tatgtctgca
    22501 attcttacat ttaatttcaa gatattaccg cccttcaaac tttttgttac aatctaatac
    22561 tatcatatta tgtattaaaa atcacttatt aagattttaa ataaactttt taaatgaata
    22621 aattctcagg aaaaaggaat tataaataca taataataca gcataggagt agatggatat
    22681 atgaacaaaa taaataaaaa tttcaaaaat atttggataa tattaggagc tgtatcgatt
    22741 tttaccatgg ttataatttt taataaaaat tgtaatgtta ataaagagac tcaaatagaa
    22801 gcttttacaa ttaagaatca tgataaaaaa tttgtatatc ctatgggaaa tataattgga
    22861 gtaaaagcaa atacagatgg tgtattggtc ttaggatatg aggaagagga tgtagactat
    22921 attggaggca tacaaatcgg tgataatata gtaaaaatag ataataaaag gataaaaaat
    22981 agtcaagatg tatcagagat tttgaataag attaaaaaaa gtaaagttga agtgactttt
    23041 gagagaaaaa ataagtataa aacagaagta atagagacta aaaaagaaaa tggcaagtat
    23101 aaacttggat tatgggtaag agataaaata tctggaattg gaactatgac tttttacgac
    23161 cctagtatgg aaaagttcaa agcaatcggt catgccataa aagattcgga tactaatgaa
    23221 ctattaaaga taaagcaagg atatatatat aagccagaac aattaaaaat agtaaaagct
    23281 agtaatgaaa aagtaggtaa aataaaagga gattttaatg acagcaattt aatgggtaat
    23341 ttctcaaata attcagagtt aggtataagt ggtaatatta cagaaaacca taataaagag
    23401 tttaatgtag caaataaaga aaagcaatta atagaagttg gaagaccgca agatgtaaaa
    23461 ataggagatg cagtaattct ttttgaggat aaaaacaaaa atataacaag ctatgatata
    23521 aaaatagaaa gtatagtata tgataaagga aattatagag atatggtaat aaaagtagta
    23581 gatgacaagt tattggaata cacaggaggt atcgtacagg ggatgagtgg agctccaata
    23641 atacaaaata ataaaattat tggtgcaata actcatgttt ttagagataa tccgaaaaaa
    23701 ggatatggta tttttataga tgaaatgata aaattgtagg tgaggcatta aaaaatttta
    23761 ttattttatc aattatctag gaggaatata attttggagt gtcgaatatg ctttagagta
    23821 gataattagg aagcaattgt gtaaaaagtt tagttttctg taataagaag atgtttttta
    23881 atggggggat ttttagtgga aaaaatcaaa atagttttag cagatgacaa taaggatttt
    23941 tgtcaggtat taaaagagta tttgtctaat gaagatgata tcgatatatt aggcatagct
    24001 aaggatggaa ttgaagcatt agacttagta aaaaagacac aaccggactt attaatacta
    24061 gatgtaataa tgccacatct agatggatta ggtgtaattg aaaaattgaa tactatggat
    24121 atacctaaaa tgccaaaaat aatagtacta tcagcagtag gtcaagataa gataactcaa
    24181 agcgcaataa atctaggagc agattactac atagtaaagc catttgattt tgttgtgttt
    24241 ataaacagaa ttagagagct agtttctaat agagttacac aagttgagcc aaaacctaga
    24301 ccagttcaag agactcaaat gactagaagt gattttgtta agaatgtagg aaatatagag
    24361 acagaaatca caaatataat acatgaaata ggagtaccag ctcatataaa aggatattta
    24421 tatttaagag aagcaataaa aatggttatt gataatgttg agcttttagg tgcagtaaca
    24481 aaagagttat atccaagtat agctaaaaaa ttcaatacaa caccaagtag agttgaaaga
    24541 gcaataagac atgcaataga ggttgcatgg agtagaggaa aagttgacac aataaatcaa
    24601 ttatttggat atacggtaca caatactaaa ggaaaaccaa ctaattcaga atttatagca
    24661 atgattgctg ataaattaag attagaacat agtatggtta aataaacaag acataaaaag
    24721 taaggctttt taattaaggc attggctata aatgcgtatt acaagcagcg aaacggtata
    24781 accactaggg ttataccgtt tcgctatttt aataaatata aaaattttct ttattatttg
    24841 cttactatat caatataata attttattat actatggata tagtatgtgt ctttacaagt
    24901 tgtaaactga cagtggttta ttttttaata taaatattga ctttgatgca ggtaaacttt
    24961 gtatttttaa gcgtattatg gaatatgtta aataaaaaaa tgatgaaata tagtattgta
    25021 aatgccaaag atgcaaaaca aacttaaaac atttatttta ttgttaagta atgctataat
    25081 ataatgtgat tttaataatg atagtggagg tttaaatatg agagtcgagg cccctataaa
    25141 agtagatcga aaaaccaaaa aacttgctaa aagagttgaa agtggggaaa tagcagttat
    25201 aaatcatata gacatagatg aagttgctgc aaactcttta gtagaagcta aaataaaact
    25261 tgtcataaat gcggctcctt ctataagtgg taggtatccc aataaaggtc cagggatatt
    25321 aactgaaaat aatatactaa taattgataa tgttggagaa gagttatttg aagaattaaa
    25381 agaaggagaa actatagaag ttgtagatgg aaagatatat agaaaaggaa aatttttagg
    25441 tgcaggagaa gttttagaca agtatgaggt ttcacaccaa ataaaggctg cctatgagaa
    25501 tttagctgta gaattggata gatttataga taatactata gattatgcta aaaaagaaaa
    25561 aggatttata cttggagaag tagaaattcc aaaagtgaaa actaattatg ctaataaaca
    25621 tgtattgata gttgtaagag gtcaggacta caaagaagat ttaagtacta tgctttctta
    25681 tatagaagag gtaaagccag tattggttgg tgttgatgga ggagcagacg ctttaattga
    25741 gtttggatat actccagatg tgattgtagg tgatatggat agtgtaagtg atgaagcttt
    25801 aaaaaaggct agtgaaatag tagtacatgc ttatacagat ggaagagcac caggtcttaa
    25861 gagggttgag gaattaggct tagatgcagt tgtatttcct gcgccaggaa ctagtgaaga
    25921 tatagctatg cttatagcct atgagtataa ggctgagtta atagtagcag ttggtacaca
    25981 ttcaaatatg attgattttc ttgaaaaagg aagaaaaggt atggcaagta cttttttagt
    26041 tagactaaaa ataggttcaa agcttataga tgctaaaggc gttaatttgt tatacagaag
    26101 taaattaaaa ataaagtaca tatgggcatt aatagcaaca gcattatttc ctgtgttggt
    26161 agttgcatca ttgtcaccag gagttcaaca gttcatacaa ctaatgcaat taaagtttag
    26221 agtattatta cagatgtaaa ttttaggagg ataaatatgc atattaatat gaaatattat
    26281 atagtcacta taggggctat atttatagct ttgggtatag gcatgttagt aggatttaat
    26341 ttaaataata atcaacaatt aagtgaacaa caagcaaata taataaatga tttggatgat
    26401 aagtttaata tcttaaaaga aaaaaatgat aaacttgaca gtgacttagc aagtgtaaat
    26461 agagattatg aagaggcagt taattttata aataaaaatg tagataagat tttagctggt
    26521 tcgctgaatg gtaagagtat aggaataata tctgctaatg aaaatgacga ttatacaaaa
    26581 aatatagaag acataataaa taaatctaat ggaagcattg catttaatat aatattaaaa
    26641 gaaaacataa caaatccaga aaagttaaag gaaatgtcaa ctaaactagg tacagatgta
    26701 aaaaatgcaa atgatgcagt taattatata attgacactc ttaaaaagga agatgcaagt
    26761 gacattttaa catatcttca agaactagat gtaataaagt ttaattttat tggagataca
    26821 tatctaaaat atgattccgt tgtaatagct ggtggaaatg atgcaaaaga cagtacaaaa
    26881 cagtttgaaa aaatagaaaa atttgtagtt tcaaaactta aatcagagaa taaatattta
    26941 gtagaagttc aaaatacagg agttaaaact tcttatgtag aactatattc taaaaacaaa
    27001 gttgcaacta tagacaatat tgatgaaggt ataggaagta tatctctagc gattcttcta
    27061 caacaaggta atatagttgg taattttggt agattggata ctgcaacttc attgttgcca
    27121 tctattaaat agaaataatt ttaaggagag aaaaaatgga ttcttatata agtataataa
    27181 taccagctta taatgaagaa agtagaataa aatatacatt agaaagcata ttggacatta
    27241 agcaaataaa tgaaataata gttgtggatg atggttcaat tgacaatact gctaaggttg
    27301 tatctgagct agaaaatgaa aagattaaat tttttaagtt agataaaaat agaggtaaag
    27361 gatatgcttt aaattatggt atcaagttag caatgaaaaa tgcagatatt ataggttttc
    27421 tggatggaga tttaggaagt tctgctaaag aagttgaaaa attaatagta cctatattaa
    27481 atggtgaggc ggatgttaca atagccaagt ttcctcctgc aaagagaaaa ggaggtttgg
    27541 gatttgtaaa aggtttagct aaaaattctg tgtttgagat gactggtgtt gagttagatg
    27601 caactttatc aggtcaaaga atatttaaaa gagaagtttt agaaaagttt gatgaaatac
    27661 cttttggata tggagtagaa gttggaatga ccattgatat attaaagtat ggatttaaaa
    27721 ttaaagaagt attagtaaat atgactcata atgaaacagg aagaaattta aaaggtttta
    27781 ttcatagagg gaaacagtat tatcacataa aaaaggtttt aggacaaaaa aagaaagaat
    27841 ggaggtaata tcttagtgaa agattatata ctttatacca ttttattatt aactggatta
    27901 tttggaacct atgctgtaat tccactattt aaaaatttat tgataaatgg aaatgtactt
    27961 agaccaaact ataaaaaaga tatgatacca gtaagtatgg gaattgtatt tttgcctatg
    28021 attgtaatca atggaataat acttgctttc tttactacag aatttaagaa tttagcgtat
    28081 atatttatgt ttatgtttgg tatgatatcc atgttttttg cagggatact agatgatgtc
    28141 attggaaata gagatgtaag tggtttaaag gggcatttta aaagtttatt taaaggaagt
    28201 cttacaacag gaggttttaa agctttattt ggtggatttg tagggcttat agtttctgtt
    28261 gctatatcaa aagacataat tgatataata gtaaatacac taataatagc tctatcaact
    28321 aatttaatga atcttttgga tttaagacca ggaagggcaa taaaagcata tttattcata
    28381 atggtaattg tatttttaac attgacagga tttgttcaag ttcttccatt acttatagta
    28441 ccaaatgtac ttgcatactt taattatgat ttaaaagcaa gggctatgat gggagatact
    28501 ggttcaaacg tccttggaat atctattgga atgctcattt cttttgggta tccatttaac
    28561 attagattag gatggttaat atttgtagtt attatgcata tattcactga gaaatactca
    28621 ttaactaaga taatagaaaa aaataaacta ttaaatttta tagataaatt gggaaggtag
    28681 tattatgata agcaaaagag tggggattgt tgaatcaata gtaagtcaaa ctgaaactct
    28741 agatgatata agagtaaata taaatggaga aatacaaaag gcatataatt atccaaaaat
    28801 atctggaact ataaatatag gtgacgaagt ggttttaaat acaacagcag tagaactgag
    28861 cttaggtaca ggtggatacc attttgtaat aactaattta aataatatag aatctacttt
    28921 aactgaaggt ggacatataa tgaaacttag atatacgcct cttcaaataa aagtggattc
    28981 tgttgaggaa caagaaagta tttatcatga taaatttgcg aattttaaag gattagaagg
    29041 tctttctgtt gtagtaggga ctcttcatag tatgttaact ccatttgcag cttcatttaa
    29101 aagaaataat ccaaataaaa aattagtgta tataatgact gatggagcat ctcttccaat
    29161 ttatttaagt aaaaatgtgg atactttaaa agaaaaaaaa ttaatagata gtacaataac
    29221 tataggaaat gcgtttggtg gagattatga atgtataaat atatatactg cattaattac
    29281 agcaaaagaa atactaaaag cggatgcagt atttgtcagt atgggacctg gaattgctgg
    29341 gacaggaaca aaatatggat ttacagggat tgaacaaggc tctattcttg atgctgtaaa
    29401 aaaacttgaa ggaagagcaa ttgcaattcc aagaattagt tttgctgaca aacgagaaag
    29461 acatcaaggt atttcacatc atagtatgac tgtatttaaa gaaattgtaa atgtaaatgt
    29521 agacatacca ataactatat atgatgatga aaaattgaac tttataaaag agcaaatcaa
    29581 tttaaatggt ttagacaaaa agcataatat aatttatata gataataata aaacgaaaga
    29641 ggatttagac tattttaatc taaaagtaaa gagtatggga agaaattatg aacaagatga
    29701 ggcatttttc aaagctgcaa gcacagcagc atattactta atggaggttt aagatggtat
    29761 tagaagaaaa aactattagt agtgataggg tttatacagg taaagtaatt actttaaagg
    29821 ttgatactgt tgaaattcca gggcaaggat atcaaaaaag ggagttagta gaagttggtg
    29881 gagcagtagg aatagttgca atcacagatg ataataaagt tgtgttagtt aagcaattta
    29941 gaaaacctat agaaaagcca atctttgaaa ttccagctgg taaactagaa aaaaatgaaa
    30001 gtccaaaaga atgtgctgaa agagaattaa aagaagaaac tgggtatagt gcaaaaaata
    30061 ttaagctaat acataaattt ttcacttcag caggtttttc taatgagata atgtttgtat
    30121 atttagcaac aggtcttact ccaggagaga ataatttaga tgcagatgag tttttagatg
    30181 tttatgaaat tgagcttgaa gaagcatata acatggtatt aaaaaatgac gttgaagatg
    30241 caaaaacatc tattggatta ttattagtaa aagatatgtt taaaaattaa tatttataaa
    30301 atgaataacc ctttgtccta aagcatatta ttttataaag gacaaggggg aattaaattg
    30361 agaagaacat atagaaaaaa tgattttaga gaattaaata gacaaataat tattatagga
    30421 ttgttgttta tgatgtctat tgttgttggg tcttacataa ataaaatatt gccaggttct
    30481 agtaacaata tattaaacaa tataaatcct gctgtagagt attataatct aaatataagc
    30541 ataaaagata cagtgataca aaatttaaaa tcagatgcaa tctttatggg ctcaatagct
    30601 ctattaagtc tatttgtagt aacaattcca gcagtgttgg tagcttttgt attaaaaggg
    30661 atgtcaattg gttatacaat aaatagttgt atattggcat taaaatttaa aagtataaaa
    30721 atgattttaa taatattatt taaaaatttg attattattc catgtgcaat catattagca
    30781 ttaattagtt taagttattt taaagaaatg gtttatgaat ttaaaaagaa aagtagaaaa
    30841 aatatgcaat ttttaattaa gaggtatata ctaaatatta taattataat agcactatca
    30901 ttggggttgc aattaatatt aaatactgca agtattggta ttattaagtt cctagctaaa
    30961 taaaggagtt tataatggat attatagagg gatatataga ttacataaaa gataaaaaaa
    31021 aattatcaga gaatacagtg gcatcatact ttatggatat aaaaaaatat atggaatata
    31081 taaatcaaaa aagcattaaa ctagtagata tagtagaaaa tgatattata agttacttaa
    31141 taaagttgga aaaagataat gtatcaatag ctacaatagc gaggatgata tcctctataa
    31201 aatcttttca tgattattta tttttaaatc atatatgtac aaataatcct gcaaaggata
    31261 taaaaaaacc taaaataaaa aaagaaaata taaatatact tacagaagaa gaaattgaaa
    31321 gactattaaa ttttccaaag ttaacaacac ctaagttgat tagggacaag gctatttttg
    31381 aagtattata tggtactggt ataaaagtat ctgaattagt agagatgaat atagaggata
    31441 ttgacctaga tattgattat atatattgta attcatgttg taaaaatcaa agagtgatac
    31501 cactttgtga tattacaaaa ctttacttag aaaagtatct aaaagaagca agacctaaaa
    31561 tggctgcaga aggtgaaaaa tcattattcg taagttcatt aggtcaaagg tttacacgac
    31621 aaggtctttg gaaagtcata aaaaaatatt ctaatctagc caatatagat aaaaatataa
    31681 atcctacaat gcttagacat tcatttgcta ttcatttgtt aaatgaaggt gctaacattg
    31741 ctgttgtaag taaaatttta ggaaatgtta atttatctag tttgcaagta tatttaaatc
    31801 atatagataa aaacgtgaga agagaaataa aggagaaaca tcctagaaat gatgttgatg
    31861 tagaattaca aaaagcagaa gctaaataga ctatatagca aattttataa tttagaagga
    31921 gcatatttat atgagcagag taatttggat tgtaattgat agtgttggga taggagcatt
    31981 acctgatgct gaaaaatttg gtgatagtaa ggatgtaagt acccttggaa atatatttaa
    32041 ggaatatcca gatattcaga ttcctaatat gagaaattta ggtataggaa acatagaagg
    32101 tatagatttt tttgaaagta taaaagaacc aataggttgt tttggaaagt gtaaggaaat
    32161 gtctcaggga aaagatacta ctacaggtca ttgggaaatg actggtataa tagttgacaa
    32221 accatttaag acatttgaac atggattctc taaggagata atagaagagt ttgagaaaaa
    32281 aactggaaga aaagtagtag gaaataaacc agcttcagga actgtaataa tagatgagta
    32341 tggagagcat caaataaaaa ctggagatgt aatagtgtat acatcagctg atagtgtatt
    32401 tcagattgct gctaatgaag aagtaattcc cctagaagaa ctctataata tgtgcaaaat
    32461 cgctagggaa ataatgatgg gggataatgc agtagctaga gtaattgcta gaccattcat
    32521 aggtaagaaa aaaggtgagt ttgtgaggac ttcaaataga agagattatt cactagaccc
    32581 atttgaacca acagttcttg ataatataaa agaatcagga cttgatgttt tagcagttgg
    32641 aaaaatagaa gatattttta atggaaaagg aattacagat gctatccaca ctaaaagcaa
    32701 tatggatgga gtggatgaaa ctttaaatta tatgaaacaa gataataaag gtctaatata
    32761 ttcaaatctc gttgattttg attctaaata tggtcatagg agagacccag aaggatataa
    32821 gaaagctctt gaagagtttg atagtagact tcctgaaata atggctaata tgagagaaga
    32881 tgatatttta ataatcaatg cagaccatgg aaatgaccca acatataaag gtacagacca
    32941 tacaagagaa tatatacctg ttatgattta tggcaataag attaaaaaag gttttaattt
    33001 aggagtaaaa gatacttttg ctgatattgg agcaactgtt gcagatatac taaatgtaaa
    33061 gttgcctaaa catgggtcaa gctttaaaga agatttattt taaactataa aatgataaaa
    33121 acttagctta ataggatgga gagattggaa tgtttgaaaa gatagcacaa agtagtaaat
    33181 ttataaattc taaaagtaat ataaaaccta agattggttt gatattaggt tctggactag
    33241 gtgatttagc aaatgatata gaagaccctg tcacaattaa atatagtgaa attccaaact
    33301 tccctgtatc aacagtagca ggacatgctg gacaacttgt aattggaaaa cttgaaggaa
    33361 aagaagttat tgctatgcaa ggtagatttc attactatga aggatattct cagaaagaag
    33421 ctacttttcc agtaagggtt atgaaagaat taggagtcga gattcttata gtaactaacg
    33481 ctgctggagg cgtaaataaa gaatttaaag caggagattt gatgcttata agagaccata
    33541 taaactttag tggaagtaat ccattagtag gaaaaaatga tgacagattt ggtgctagat
    33601 ttccagatat gtcagatgca tattcttcta aatatgtaga tgtagttaaa aactgcgcta
    33661 aagaatgcaa tatagatgtc aaagaaggcg tttatatgtt ttttagtggt ccaaactatg
    33721 aaacaccagc agaagtgaga atggcacaaa ttttgggagc tgatgctgta ggtatgtcta
    33781 ctgttccaga agtcatagta gcttctcact ctaatatagg agttattggt atatcttgta
    33841 taacaaatat ggctgctggc atcttaaatc aaccactaag tcatgaggaa gtaatagaaa
    33901 ctactcaaaa agttaaatca gaatttatga atttagttaa aacaacggtt aaaaatttat
    33961 aagttaaagc ttattataga ttttttgagg aagtttataa taaaatagtt aatgtttaat
    34021 ttgaaaagaa ttaataaaaa gctgtcttga tatagaaagt tatttctaat aaaggacagt
    34081 ttttttataa tactacaaca tttgaaagtg ttttaaagtt taatatttat ttaaattatt
    34141 ataggttaga atggagattt caattatgag aatgtatgat attataaaaa aaaagagaga
    34201 taacctagag cttagtaaaa aagagataaa ctactttata gaaaactatt caaagggaga
    34261 tatacctgac tatcaagctt ctgcactatt aatgtcgatt tatttaaata agatgaataa
    34321 acaggaaact gtacatctta cagaagcaat gatgaactct ggagacatga taaacctatc
    34381 tgagatagat ggaataaagg tagataagca tagtactggt ggtgttggag ataagactac
    34441 catagctctt atacctttgg ttgcttcatg tggagcacca gttgcaaaga tgtcaggtag
    34501 aggtcttggt catactggtg gtactattga taaattggag tctataccag gtttttcgac
    34561 agaaatggag ataagtaaat ttattgattc tgtaaataag tctaaaatag cagtttgtgg
    34621 tcaaagtgca aaggtagcaa ttgcagataa gaaaatatat gctcttagag atgttacagc
    34681 aactgttgat aatatatcat tgatatcatc aagtgtaatg tctaaaaagt tagcttcagg
    34741 agctgatgca attgtgctag atgtaaaaac tggtgatgga gcatttatga agacattaga
    34801 agagtctttt gagttagcta aatctatggt tgatataggt actggtatga atagagatac
    34861 aatagggatt gtaacagata tggatgaacc tttaggtttt gcagtgggaa actctttaga
    34921 agttatagag gcaattgaga cattaaaagg aaatggacct aaagaccttg ttgagctatg
    34981 cgaagtatta ggagcatata tgttggtttt atcaaaggta gcacatgatt ttgaagatgg
    35041 tgttgaaaaa ataagagaat caataaaatc tggtactgct atacaaaaac tcaaagagtt
    35101 tatagagaat caaggtggta ataaagatgt agtagatgat tattctttat tcaaaaaatc
    35161 aaagtataaa tatccagtta aatcaactaa atcaggttat ataagcaaaa ttaaagctga
    35221 agatgttggt gtatcggcta tgattcttgg agcaggtaga gaaactaaag atgatatttt
    35281 agatttatca gcagggataa ttttagaaaa gaaagtagga gattatgtaa atgaaggtga
    35341 agttttagct tacatgtatt ataatgatga acaaaagctt tcattggcaa agggaagatt
    35401 tattgattcc tactctattg tagattcaaa aatagaaaaa aataaattaa tatatggtat
    35461 tgtaactaaa gaagagatta aaaagtttta gaaaatttta gaaaagtgat tataaaaggc
    35521 ctaacatgtc aaaaataatg ttaggttttt tattttttga taaactataa attataccaa
    35581 aaatatatat tttaaaacat tttttacaat ataaataagt ttattctaca agatgattga
    35641 aaattaaagt atctagtttt ataatagaaa atggataaat atacataagg agtagattat
    35701 attatgaact ttaaagaaaa tagattgaaa atagcagtat taataattgt tattttgatt
    35761 atcttagcag gtatatttgt gtttatacaa ataggtcctt atgataaaaa taacaaaaaa
    35821 gatgtaatta tcgacgttcc aagtggagca tctgttggaa aaatatctga catattatat
    35881 gaaaataaat taataaagaa tgaactatta tttaaacttt tggtaaaggt tagcaataaa
    35941 gctccgtcta taaaatcagg aacatactta cttaatcaat cgtattcaaa caatgatata
    36001 ataagtcttt tagtttcagg taaaatatat caggatggta taaaagtgac tattccagag
    36061 ggagctacat ctaaagaaat tatagcaatg ttagtaagta aaaatttagg agataaagct
    36121 acttttgaaa atttaataaa aaaacctcaa gaattttatg ataaattttc atatttgaag
    36181 gaagatggaa taacctcttt agaagggttc ttgtatccag aaacttatta ttttaattca
    36241 aagaaacaat cagaagaaga tattttaagc gaaatgttaa aagtatttga cagtaaatac
    36301 acggacaagt ttaaaaagaa acaaaaagaa ttaaatatga ctcttcaaga agtaatggaa
    36361 atggcatcaa taattgagaa agaagctgtt cttgacaaag acagacctat aatagcaagt
    36421 gtgttttata atagacttaa ggtaggtatg cctcttcaat cagatgctac aatacaatat
    36481 atatttgaag agagaaagaa aattgtaaca tatgatgatt taaaaattga ttcaccatat
    36541 aatagttata aaaataaggg attacctcct acaccaatat caaatcctgg tataaaatct
    36601 attgaagcag cattatatcc agagaaaaca gactatttat attttgtagc taagattgat
    36661 ggtggaaata attatagtac aaactatcaa gaccatttaa aatacgttaa agaatataaa
    36721 gaagcaaggg ataaacaatc aaaagataca aaagcaacaa ataaagaaaa tacaaaaaaa
    36781 taaaccatga ttataaaaag tctaacaggg taagaataat gttgggcttt tttattgaaa
    36841 attgttattc ttagatttat aataaagaaa gtgtaaatat atataacaat atgaatttaa
    36901 aaaataataa attaatataa aacttggata tttattattt tagggagtgt ttttatgagt
    36961 aatatagtta atgatttggt tgaagattat ataagagtga cattaaaaga aaaagaagga
    37021 tttttaaaag atttagaaat ttatgcagag gaaaatagtg ttcctataat acataaagaa
    37081 gtttcagatt tattaaaagt cttgttaaag gttcaaaaac caaagagaat tttagaagta
    37141 ggatgtgcaa ttggttattc ttctattttt tttgcaacta ttataggaaa agatgcagat
    37201 ataataactg ttgagagaaa tgaaaaaatg atagaaaaag caaaagaaaa cataaaatta
    37261 gctggatttg acaataacat aactatacta gaaggagatg cagaagaaaa acttagtcaa
    37321 gtccaaggtg aatttgacct tatatttata gatgcagcca aaggtcaata taagctattt
    37381 tttgatttag ttatagataa attaaaagat gggggacttt tagtatctga caatattttg
    37441 tataaaggta tggttgctca tgatgatttt gttgtaagaa gaaaaaagac tatagttaaa
    37501 agaatgagaa agtatttaga ttatatatgt aattgtgact atttaagcac atcattggta
    37561 ccaataggtg atggtgtagc actaagttat aaagaaagta aaagaggtga tgtagatgga
    37621 attaaataaa gtagaacttt tagctccagc aggtgatttg gagagattga aaatagctat
    37681 aacatatgga gcagatgccg tttatatagg tggagaaatt tttggaatga ggtctgcagc
    37741 gaagaatttt agtaaggaag atatggcaga gggagtagct tttgcacatg aaagaggcaa
    37801 gaaagtattc gtaacagtaa atataatacc tcataatgaa gattttctac aattagaaga
    37861 ctatttgttg gaactagaag agataggaat agatgcagtt ataatagctg acccaggagt
    37921 gcttagtgtc attaaaaaag taattccgaa tatggaaata catttaagta cacaagcaaa
    37981 tacaaccaat tatctaagtg caaacttctg gtacgagcat ggaataaaaa gagttgtagt
    38041 ggcaagagaa ttatcttttg atgaaatata tgagataaga gcaaaaacac ctttagatat
    38101 ggatatagaa gcgtttatgc atggtgctat gtgtatttct tattctggca gatgtttaat
    38161 aagcaactat atgactggaa gagatgcaaa caaaggttct tgtgctcaat cttgtagatg
    38221 gcaatatcat ttggtggaag aaaaaagacc tggtgagtat tttccaatat acgaagatga
    38281 aaggggtaca ttcttcttta actcaaaaga tttatgtatg atagagtata taccagagtt
    38341 aatcaaatct ggtataacaa gtttaaaaat agaaggtaga atgaagactg catactatgt
    38401 agctactgtt gtaagagcat acagaatggc aatagatgaa ttttacagag acccagaaaa
    38461 ctggaagttt aatccaatgt ggatggaaga actaaaaaaa ggtagtcata gacattttac
    38521 atcaggtttt tacttgaata aaccaacaac agaagaccaa aattatcaat cagcatcata
    38581 tgttagaaac tatgatttta taggtatagt tagagaaaca gaagatgaag atggtcttat
    38641 tgttgttgag caaagaaata aaatgtgtgt tggtgatgaa atagaagtta tgggaccata
    38701 taaagaaact atgtttacta aaattgaaga aatgtataat gaagaaggag aagctataga
    38761 atcagctcct catcctagac aaatagtgaa gttaaagcta tcagtaaaag ttggtaaaga
    38821 ttatatgctt agaaaagtta tagaagaaaa ggttgaagaa taataaaaaa ttgataaaaa
    38881 tagtattagc cccttagaaa ttgtcaataa tgaaattatc taaggggtga tttcatgtca
    38941 aaaaagaaga ctccattttt gaaaaaagtg ggaaagagaa gctggtgtat atttactata
    39001 attttaataa tttatagtgt tttaatttat aggctagttg atatacaagt attaaaaggt
    39061 gataaataca aacaaagtgt tgaatcacaa agcgttgaga aagtcgagtt aaatagtgga
    39121 agaggaataa tctatgatag aaacaacaag aagcttacag atacaagtaa atctcaggta
    39181 ttaattgttg aaaaagaaaa attaaataat aactataaaa ttttggagct tattaagaaa
    39241 gctactaaga tgaatgattt agatatatat aaagctgtac aagagcaatt gacacgccct
    39301 ataatacaga ttcagactaa aaatatcgat atcgataaga gtatgaaaaa agaacttgag
    39361 aaaaatggaa taatggtaga agaaaaaact atgaggtacg caaaagatgg tttattatca
    39421 catacaatag gatatataaa agaagatgat aaatcaggtc aatcaggaat tgaaaagagt
    39481 atggatagtg tacttagaaa ttcaaatgaa aagtatataa gtgcttttaa agctggagat
    39541 gcaggaaatg aaaaatctct aaacatacta aagggaagtg taaaaactgt tgataataag
    39601 gataaagata gacatctaaa aactactatt gattataaca ttcaaaaaaa attagaacaa
    39661 atactaaata aagaagaaaa tcctacagca gcaataatct cggaagcatc tacaggtgaa
    39721 attcttgcaa tgtgctctag acctaatttt gaccaaaatg acatatcaaa aagtttaaaa
    39781 ggaaaaaatg gagaatttga aaatagagtt ataaaagcga catatccacc agggtctgta
    39841 tttaagatgg tagttttatt ttctgcactt gaaaatggtg ttatagatga gaactatact
    39901 tacaactgta caggtaagac taaagttggg aatactaatg aaatactgcg atgtaataaa
    39961 agagatggtc atggtttcca aaacttaaga caagcttttt ctaattcatg caatcctgca
    40021 tttttagata tagctatgaa acttggaaaa gaaaagatac tcaaatctgc tgaaaaatta
    40081 catttatttg aaaaggttga tattggtctt gatgaagaaa aaattagaga agctccaaaa
    40141 aacatatcaa taagaaattt agctataggt caagaaaata tagaatttac accacttcaa
    40201 ataaatcaaa tgactcaaat aatagctaat aatggaacat tcaaaccact atacttatat
    40261 aaaagtcttg tggacaataa tatgaataca ataaaaactt ataagtcttc aaaaaaagaa
    40321 gaacttattt ctccttatgt gtgtacacaa gttaaagaat atatgaagtc tgtatctagg
    40381 actggtacag ctaaagacct aaaagatatt gaaggtggtt gtggagttaa aactggtaca
    40441 gctcaaagta gtttaaataa aaaagctata gaccacggat ggataacagg attttatcct
    40501 gaagaaagac ctaagtatgt aataactgtg ttagtagaag gaactcaaaa aggaaataaa
    40561 tctgcaacac ctatatttaa ggaaatatgt gaaagtataa aataaattat gtactataaa
    40621 atttaaggat atatcgtaac tttgatttta gatagaaaat tttgtctaat gatgtacgat
    40681 atatcctttt aaaatatagt attaactttc ttgaacaagc tctttttcta gttttttaag
    40741 agcttttttt tcgattctag atacatatga ccttgatata tcgagttttt gtgcaatttc
    40801 tctttgtgtc ttatagccat aaggagttaa cccatatctt aactggacaa tttctctttc
    40861 tctaggagta agaattttgt ttagttgttc gtaaagtttt ccaacttgaa cttttagttc
    40921 aacttcatca agaacatagt tagcctctgt accaagtata tctaaaagac ttatttcatt
    40981 accttcttta tctgttccga tagggtcttg tagagatact tgagttttat tttttttgtt
    41041 agatctaata ttcattaaaa tctcattttc aatacatcta gaagcatatg ttgctaatcg
    41101 agttccttta tctatgttat aagtttctat agctttaatt aatcctattg tacctatgga
    41161 tattaaatca tcctgatctt ccgttgaatt attatatttc tttgctatat aagctactaa
    41221 tctcatgttt ctttcaatta atgtatcttt tgcactttta tcgttttcta ttttaaattt
    41281 agttaaatac tcaatctctt cttcaggggt taagggtttt tcaaaagatt taagagctgc
    41341 caataggtca cccccttttc aacggtcact ttaaattata tgaaggagac ttatccaagt
    41401 tgactgttat ctaataaaat aatatgcata tttttagaat ttaaaacata tatttgcaaa
    41461 cttttgaatg gaatatgaca taaagagtgg gaatttaaat ttaagttttt gttgtatata
    41521 tatgaaataa ggcgaagaaa ttattgattt ataaagtata aattggaatt gaggtgtcaa
    41581 taaatataaa aggaaatagt atttatatat gttttggtgt tgaagtaaaa gattaatgca
    41641 aagcattaga gtctataaaa atatgaatat aggaggtgta attttacaat acttaaaaaa
    41701 gtaaatgtaa ttaattgttt gtataaagtt gattacaagc aaaagtatca gtcatagtta
    41761 atactgttaa gggtgaaggt ggtgtgttaa tttatatatg tatgggaaga aattttgatt
    41821 acaatgaaaa aacttacaaa agatatttat gaaaaaactt atagttaaat gtttatatat
    41881 aaaatattat ttaggggttg tttttattat tgaaaataag aaagagtggt tttatatcaa
    41941 ttgaatgtat aataagtatt gctatattat atgtggctgt ttatttagtt tctacatcat
    42001 tgtataattg ttatagtttt atcagtagaa atatatctga cagagaaatg ttaagtacag
    42061 caaaaaaata tatagaagat gagaagtata gaatacaaaa tagtaagtat gagttaattg
    42121 aagataagat agaaaaaaat tacataaatg gatatgaaat taacagtaaa atagagcaaa
    42181 ttttagatta ttatcaatgc tatgaaataa atatagagat aaaaaatgaa tttaaaaaac
    42241 tgaggtttaa tagctatgtt actagaaaat aaaggctcta tattaataat aacattgata
    42301 atattttcta ttataagtac tgtgtgcatt atgtgtatag gtttaattta tacacataat
    42361 aatatgtttg agttaggata caaggacatt caactaaaag aaatttcttt aagtggaata
    42421 gaaatatcag taagtaatgt actttcttgt gttgaggaag ctattgatgt atctaataat
    42481 gaagacgaat ttagagaata tttcttagga aataatatga ttgaatgtat aaaaaaaata
    42541 aaagatacat cttatagtag tttagaagaa ctttctttaa aagtagaaaa taatactatt
    42601 tatgacagag gagatttttt tgaatttgat atagaaagta atgttagaga aaaagaattt
    42661 ttaaaagcaa taaaagtaag agtgaaaatc aaaaatccat ggataggaat tgacattgaa
    42721 gattatatgg ataaatctaa taacaataaa attgatgaca aaacattcaa taattataaa
    42781 gcatctgaaa ataatgaaat tctaaatcaa tttaaaaatg aaagtgaaaa tataaggaat
    42841 caagaggttg gtggatttac agaagaagat gaagaagatg aagaatatga agaagaactt
    42901 aaacaaaaca taattgaaaa tataaagaag aattttgatg aaaaaaacct agtacaggta
    42961 tataagtata ggggtgtata actttgaaaa aagaaggaat gtgactgtaa tagaattaat
    43021 agtatctatc agtataatac tcttgatagt tacattatct tttccaaaag ataatcttga
    43081 aaatcataaa ataaatttat ttgcacgtca actttgttca gatataaggt atgttaggcg
    43141 aattaatatg tttagcaact acaatacata tatatattat ataaatcaag gtaaacacga
    43201 aggttatgta ttgagaaaag atggtaaaaa cattaaatca ataatgctac ctaaaaatgc
    43261 aaaaatagct aggagtattg aaataataaa gtttaaatct gatgggtcgc ctcaaaaggc
    43321 gtctacaata gagatatata ataatagatt aagaaaaact ataacaataa cacctgtaag
    43381 tggaagagta cttctgaaag agggaaaata tgaaacataa atatggttac ttgcttttgg
    43441 aaagtgtcgt atcattgtct tcaatggtga taattatact agttttatat tctatatttt
    43501 tgtctacaat aaacttaaag ttaaaagtag aagataagat agaactacaa caacaaagtc
    43561 ttgaaatcat aaaatctatg gaaggaatta ttagcaattc tatgggaata atgaatgtat
    43621 ctaattatga agaaacattt aaaaagacaa catctataaa atgtagatat ttagatgaaa
    43681 ataataatga agaaggcata agtaataaag aaattatatt gaatgaaaga cgaaataagt
    43741 tatttgtaaa ttctttaaat ggcgaaagta gtcaagcagg tggttatgaa attggagatt
    43801 atgtagatga gatgtatgta tcaattacta ataatggtca atatgttaat attaaattga
    43861 aattatcaaa acgtagtcaa aaatatgaga ctgatttcaa aataaaagta tggaatttta
    43921 gtgaaagtat atagaaatac ataaatttaa gagaaagaag tgacatcaat aagtcatata
    43981 aaatataatt attgagaact atataactaa ataata
//
LOCUS       DAMJYR010000024        43639 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.024, whole genome shotgun sequence.
ACCESSION   DAMJYR010000024 DAMJYR010000000
VERSION     DAMJYR010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 43639)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43639)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43639
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.024"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            267..731
                     /locus_tag="PC471_001225"
     CDS             267..731
                     /locus_tag="PC471_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HDF2788363.1"
                     /translation="MATMTDIIEKFIKDLMEEDNSIQIQRNELANLFSCAPSQINYVL
                     TTRFTIDRGYYIESKKGGGGYVQIEKIRKSKDGHIRELLNEKIGSQISYKKAKELLEG
                     LKESDLINERELKLILYAIDDKSLCMPIYELKEKVRSNILKNIIIGLFSIEE"
     gene            738..1232
                     /locus_tag="PC471_001226"
     CDS             738..1232
                     /locus_tag="PC471_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UvrB/UvrC motif-containing protein"
                     /protein_id="HDF2788364.1"
                     /translation="MLCQKCNKNKASVYYNKIVNGEKTEMYLCSECAKENTEMNFNLD
                     MPFSMMDIFSNLGFQPKKELEEKLVCPKCNTTYSEFKNNGRFGCSECYNAFSSQVNPM
                     LQNIHGHIEHTGKAPKKSFYKISVENEIKELKEDLDRAIKNEEYELAAQFRDKIKYLK
                     GSID"
     gene            1248..2273
                     /locus_tag="PC471_001227"
     CDS             1248..2273
                     /locus_tag="PC471_001227"
                     /EC_number="2.7.14.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein arginine kinase"
                     /protein_id="HDF2788365.1"
                     /translation="MKENIVMKSRVRLARNLNNYPFPNKLDKECAMEIIEKVKNAFIN
                     SNLEQKEEFDFYKIEDLDQSKKMLMVEEHIISPDLAENDKSAVIVKKDKTISIMINEE
                     DHIRIQTICDDLNLEYAYSVANEIDDLLESSLEYAFNTKLGYLTSCPTNTGTGMRASV
                     MMHLPALSQLGYMDELYKISSQIGIAIRGIYGERTEALGNIYQISNQLTLGRTESNII
                     ENVSGLTKDAISKEIKAREILQKKLGIKLEDKIFRSIGTLENSRVMSSAEAMSHLSNI
                     KMGIEMNYIDKLDLRAIEQLMIGIQPAHQSIMYKSDDVENRDINRATYIRETLEKLRG
                     GMNCELQ"
     gene            2260..4707
                     /locus_tag="PC471_001228"
     CDS             2260..4707
                     /locus_tag="PC471_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HDF2788366.1"
                     /translation="MNFNRFTQRAKKAIDLAFESAKSLGHNIVGSEHILLGLLREEEG
                     IAAKVLSKVGFTEAYLEGKIVDMEGKGEEISEDIVLSPRSKQILELSGMFANKLKTNY
                     IGTEHILLAIIQEGEGIANKILNYAGVNDRTLAQLTIDMMGISDKNQYKAENSYTGNQ
                     NQAESKVLDKYGRNLTLYAKQNKIDPVIGREKEIQRVIQILSRRTKNNPVLIGDPGVG
                     KTAIAEGLATNIALGNVPETLKNKTLYSLEMGSLLAGAKYRGEFEERIKEVVDEVVKN
                     GNIILFIDEMHTIIGAGSTGEGSIDASNILKPALARGEIQVIGATTIDEYRKHVEKDS
                     ALERRFQPVMVDEPTKEDSIKILEGLRDKYEAHHKVKITDDAIKAAVELSTRYISDRY
                     LPDKAIDLIDEAASKVRLKENTPPSEIKKLELEIENIDKEKEEAVRCQDFEKAAKIRD
                     EQGLLKKQLEDVRERWNKSSKHSDLVDGEVIAEVVGLWTGIPVNKILEEEADRLLRLE
                     EILHNKVIGQEQAVKSISKAIRRSRAGLKDPNRPIGSFLFLGPTGVGKTELSKALAEV
                     QFGDENQIIRIDMSEYMEKHAVSRMIGSPPGYVGHDEGGQLTEKVRRNPYSVILFDEI
                     EKAHPDVFNILLQILDDGRLTDSKGRTVDFKNTIVIMTSNVGASTIGRQKTLGFSIAK
                     GDEEEKSQYEKMKENIMGELKQRFRPEFLNRIDDIIVFHSLNENHISKIVLLMAAKLQ
                     ERLKEMDIKLEMSDEAVKLISKSGFDLEYGARPLKRALQKELEDELSEAILKGNVKKG
                     SNVVAKVKDEKIVFETK"
     gene            4887..6260
                     /gene="radA"
                     /locus_tag="PC471_001229"
     CDS             4887..6260
                     /gene="radA"
                     /locus_tag="PC471_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HDF2788367.1"
                     /translation="MAKIKTKYVCQSCGYETAKWLGKCPECTKWNTFVEEIDQKSTKK
                     EVFIIDKSSSKPVSINSIESKEEERFTTDINELDRVLGGGIVKGSLVLVGGDPGIGKS
                     TLLIQVSSNVANLGKTVLYITGEESESQIKMRAKRLGINSEKLYIFAENNLSIIESYL
                     ESVNPELIILDSIQTVFSPEISSAPGTVSQIKEGTSKFMKISKKMGISTFIVGHVTKE
                     GSLAGPKLLEHMVDTVLYFEGERYNTYRLVRAVKNRFGSTNELGVFEMRDLGLVELDN
                     PSKILISEKPKDVAGSVIISTVEGTRPMLLELQALVSPTSFGIPKRTSTGVDYNRVGM
                     LLAVLEKRVGLQIQNQDVYINIVGGIKINEPSIDLGIAISVASSFRNIPIDEDIAVTG
                     EVGLTGEVRAVSFIEKRIAECKKLGFKKIVVPRSNYDVVKETKGIEIWPVDNLRQAIN
                     IVLGRNQ"
     gene            6263..7333
                     /gene="disA"
                     /locus_tag="PC471_001230"
     CDS             6263..7333
                     /gene="disA"
                     /locus_tag="PC471_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA integrity scanning protein DisA"
                     /protein_id="HDF2788368.1"
                     /translation="MENFLDNKNMLYALKMISPGTPLRLGLNNVLRAKTGGLIVIATN
                     EDVMKIVDGGFAINAEYSPSYLYELAKMDGAIVLSGDVKKILFANAQLIPDYFIETSE
                     TGTRHRTAERVAKQTGAIVIGISQRRNVITVYRGNEKYVVEDISKIFTKANQAIQTLE
                     KYKTVLDQAVTNLNALEFNDLVTIYDVALVMQKMEMVMRVTSIIEKYVIELGDEGTLV
                     SMQLEELMGTTRIDQKLIFKDYNKENTEIKELMKKVKNLNSEELIELVNMAKLLGYSG
                     FSESMDMPIKTRGYRILSKIHRLPTAIIENLVNYFENFQQILDASIEELDEVEGIGEI
                     RATYIKNGLIKMKQLVLLDRHI"
     gene            7422..8516
                     /locus_tag="PC471_001231"
     CDS             7422..8516
                     /locus_tag="PC471_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HDF2788369.1"
                     /translation="MIRKVTRILFILLGFTIGITTYLTLMKDFEILTFGKETYGYIAA
                     VVAGIIIAILGYLIEPWVVNKVKEIAKIVDKELSKYPQTDILLGSMGLIVGFVIAYLL
                     SGLVNRIPIVGGILSLLLYLFLGYLGMKVALKSKNDLFNIGKLGRLANPIKDKDKDKE
                     NKKEVKAIPPKVLDTSVIIDGRIADICKTGFIEGKLIIPAFVLEELRHIADSSDDLKR
                     VRGRRGLDILNIIQKELNIEVEISERDFDDIAEVDSKLLKLAQVLNGKVVTNDYNLNK
                     VAQFQGVEVLNINELANAIKPVAIPGEDMVVQVVKEGKEAQQGVAYLDDGTMIVVDGG
                     RKYMNETIKVLVTSVLQTPAGRMIFAKPKN"
     gene            8645..10822
                     /locus_tag="PC471_001232"
     CDS             8645..10822
                     /locus_tag="PC471_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2788370.1"
                     /translation="MRKKIISFLATFIIILTSASQYSFADDISTRGKVIFIDMNRTSM
                     SNMLRIKSLREELDNRGYIGLMNIRGDKGSDDRRSYASMGAGGRANVANEEDINFESS
                     SKDRNIVFESATGKSAKGINNLTINKSINENLNFGEYGSVLGSLGQSLSDNGLKASVL
                     GNSDIIENGQLIKNRNLCLTAMDEYGRIPNGNVDTINKKDLSMPYGISTDYDKLIVET
                     KEAYKNNDVIFVELGDTYRLDLYKPNLNEKTYESMKDNIEKNIDIYLKNIFSMVEEND
                     TVYIASAFPSDLDYKNKRRLSPIIKLNGEGKGILSSSTTRREGIVTNLDVGVEILDNF
                     NIKNQNMVGRKYELINRDDNKEFLMDEYQKIVSISSIRSTILNGFVSIVFLSWIVAMI
                     AILFRKHISKNYKETTFFILKELLKIGIVMPLSFMITPIMNFKTPLAISLGIIIITLT
                     IYLISKVLIKNDLKNMLFFTGLTIVIMVIDAGFGSYLMKSNVMSYDCIIGARYYGVGN
                     EYQGVAIGSAIFTFAILLTYKNIPKWSIIVFSLVILITSASPIMGANVGSAISECVAF
                     LLFILLIYNVKIDFKKIVLLGIAVVFVLGVFVAIDMILGSNSHLGMFVKEIYFNGPGE
                     IIQTFSRKIEMNLKLAQTSAWVNILLTGIGVILVLMINQIRYFKQLMDEYPIVFKGFI
                     ASIAGCLVTLLVNDSGIVSSATAFIYVIVPMITLSINLTALKE"
     gene            complement(11002..11853)
                     /locus_tag="PC471_001233"
     CDS             complement(11002..11853)
                     /locus_tag="PC471_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF2788371.1"
                     /translation="MNRNNFSHEIIEPIDNLDVNFKLFDDSGSYVANHWHNSLEIIYI
                     TSGDLQINMEGYTYNLKANECMFINSGIIHSTRCIYQNTSILLQVPLSFLNKYIPDFK
                     NCYFDFKVNANDNNYKKNSSKVKLILENMREIKLSSPPAANLLFTSLLFELLFELYTN
                     FKISVGNKNMKKTVLDLSKFEPVLEYTKINYKMPISINKIAKVAHLQPEYFCRKFKQY
                     MGQTYLEYLNDVRISHIYKDLINTNDTLCSILEAHGFTNTKLFYRIFKEKFKCTPKHI
                     RKNLPKI"
     gene            12070..13236
                     /locus_tag="PC471_001234"
     CDS             12070..13236
                     /locus_tag="PC471_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HDF2788372.1"
                     /translation="MYIKGVNLGGWLVLEKWMTSSLFEGTEAEDEYYLPRQLSREAYE
                     SRIKTHRSEYITERDFAMIKSMGFNSVRIPVPYFIFGDCEPFIGCVKELDKAFAWADK
                     YGLSILIDLHTVPGSQNGFDNGGISGICSWSQNPEYVAFTLNVLERLAKRYGMRHELY
                     GIQILNEPITERMWNIMNVPNRFKAVDKEMARGSKPNSLEFLRDFYIKAYKVMRPYMR
                     EENVIVFHDAFELKAWKDFMREEEFKNVVLDTHQYLMLAEADGCEQSIDSYLKYIREN
                     YAKDILQMQKYFPVICGEWSLFNSYACGIDTNGGQSPLNGIESNIDKLSKDDKRELYR
                     KIAKAQLDAWRNGSGHYYWNYKLLLDTVNEEGWIGWDSWDLVKCVAQEWYPIEY"
     gene            13258..14904
                     /locus_tag="PC471_001235"
     CDS             13258..14904
                     /locus_tag="PC471_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HDF2788373.1"
                     /translation="MIKNPILPGFNPDPCICRKGDDYYLVVSSFEWFPGIPVYHSKDL
                     KNWELYTHILTDETKIDLKKLPSSKGIWAPCLTYCEEEDLFYIVYGIMNSMNARYFDV
                     DNYLITSKDIKGEWSEPVYLHSSGFDASIFHDDDGKKWIASLDWETREGYEKPGVICL
                     VEYCTKKKEIVGYPKRIWSGGTDRGCIEAPHITKRGDYYYIMCAEGGTGYGHSVTMGR
                     AKNIWGPYEKDSMNPIVTSIPGDFYERHDPDHLKPKYYNPESKLQKSGHGSYIETTSG
                     EVYLVHLTSRPFVPELRCTLGRETAIQKMKWTKDNWLRMEDESNLAKEYVSESKLEEH
                     LVSSIPSFDDFDSNELGLQYYAPRISPLSFADVKSRPGYVRIRGQESRTSLNKVSILA
                     RKLTSVYARITTKMEFYPEVHQHSAGLIMYYDNMNYINLRKYYSETLGQSALSIIHLE
                     NGEKTEFLNTRIPIKDIPIYLRLYVQGRKSYFEWSYDEKNYQRIGKVFDTTKFSDEYC
                     KYGEFTGTFIGLTCADRVKHKHYADFDFFEYIVDESKDVD"
     gene            15001..16374
                     /locus_tag="PC471_001236"
     CDS             15001..16374
                     /locus_tag="PC471_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDF2788374.1"
                     /translation="MNTEVNVKATTIRYEKISFFERISYGCGDLGCNIIYSAMSAFLL
                     FYYTNYADVSAAAVGSIMLVSRILDGFSDLTMGIIVDRTKSKYGKARPWILRMAIPFA
                     IAAVLLFSVPSNLGITSKLIYIFITYNLVSTVIYTAINVPYATLNSLITQNQYERGVL
                     SIFRMILATCGTLLINGLTLPLVEYFGNNLSAWTKTFFVFGIASIMVFFITFTGTKER
                     VKAVKQDRNEVIPFKIGIKSLFRNKYWIQITLCLVCIFIVFAINGGSSVYYAKFILGD
                     EKLFGPINMVSNISQIIAMFMVAPFIKKFGKRNVLIVGSIILISSNVMFIIVGQNHIG
                     VIFASAIKGIGSAGIASTMFAIVSDTIEYGEWKTGYRTEGLINSASSFGFKVGNGLGS
                     AILGAVLSIGGYVGTSATQSDLAIVSIKVCFIYLPIFITILQIIIMSFYKLDKEYSTI
                     LNELNTK"
     gene            16819..17772
                     /locus_tag="PC471_001237"
     CDS             16819..17772
                     /locus_tag="PC471_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HDF2788375.1"
                     /translation="MKKIGDLRLMMKCCSLYYEDNLNQQEIANQLGISRPTISRILKE
                     AFEQGIVKIQIVDVLKNDYQKIERSLERKYKLKEVIVVDDKQDALTQKQELARAVSEY
                     LTRVVKENDIIGVSIGTTLKEIPRYVEKSNCKNVTFIPLLGGIGDNEIDIHANQIAVS
                     LARAFGGDFKLLHAPAVMSDLSTKEKLCKDEKIKEVLDLIDKTTIAIVGIGNPMSLNS
                     TIMASGYMNEADIEDLKKYNSIGAICLQAFDKEGKTSILEFNQRVLGVKLEDLKKIKR
                     TIGVASGDEKIEAIKASLKAKFINSLAINHSLALKLLEDCD"
     gene            17810..18772
                     /locus_tag="PC471_001238"
     CDS             17810..18772
                     /locus_tag="PC471_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HDF2788376.1"
                     /translation="MSISKETLLEMYKRMNQARKFEEKVSWFFARGMVHGTTHLSVGQ
                     EASSVAAVMALEKGDLVSLTHRGHSQFIGMGIDLNKMMAELMGKETGFCKGKGGSMHI
                     ADIESGNLGANGVVGGGLTIAPGAALTQQYKKTGKIVLCSFGDGASNEGTFHEGINLS
                     SIWKLPIIFYCENNLYGMSTSIKRHMNIESIATRAASYGIEGISIDGYNPIEVYETVQ
                     KAAEKCRRGEGPVLIESRTYRWLGHSKSDANVYRTKEEIESWKAKDPIEFLKNYLIEN
                     NLSNEDELDKIQEFAKQSIEDAVEFAQNSPNPKIESLLEDVYAD"
     gene            18797..19783
                     /locus_tag="PC471_001239"
     CDS             18797..19783
                     /locus_tag="PC471_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HDF2788377.1"
                     /translation="MSTRELTYAQAIKEAMSEEMRRDENVIFMGEDIGIYGGAFGVSV
                     GMIDEFGPERVRDTPISEAAIAGAAAGAAATGLRPIMEVMFMDFVTISMDAIVNQAAK
                     MRYMFGGKAQVPMVVRCPGGSGTGSAEQHSQSLEAWFCHVPGVKVVAPSTPADAKGLL
                     KAAIRDNNPVIFVENKLLYRKKGFVPEDDYVIEIGKADIKREGTDVTVITYGRMLQSV
                     EEAAETLSKENINVEIIDLRTLYPLDKETIVKSVCKTGRVLICHEAAKTGGLGGEISA
                     LITESESFDYLDAPVKRICGKDVPIPYNPELEKAVVPRVDEIEEAIKSLIVR"
     gene            19796..20842
                     /locus_tag="PC471_001240"
     CDS             19796..20842
                     /locus_tag="PC471_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HDF2788378.1"
                     /translation="MVANKIKATPAARSQARKDNIKLDRLIGSGENGRIHLVDVLNYL
                     KDNKANTTPLARRIAEDLNIDLETIVGTGYNGKIRKCDVEKLTAKETIVSTNTSKSSE
                     KKELKIENENSSMFNTVEGIFEKPNPMRATVAKRMSESYFSAPVFTFNIEVDATELKV
                     LRAKLIDTVKESTGVKLTMTDLIVMAVSKILPNHQALNSAWTDEGIFRYKDVNIAIAV
                     GLDEGLYVPVVKNANKKSLKEIAKESKELAEKVKTGKLMPADQEGNTFTISNVGMYGI
                     TTFTPIINMPSSAILGVGATQDKFVPVNGEAKIKPIMNLSLTSDHRVIDGTVAAKFLK
                     DLKELLENPLSMLV"
     gene            21009..22739
                     /gene="lpdA"
                     /locus_tag="PC471_001241"
     CDS             21009..22739
                     /gene="lpdA"
                     /locus_tag="PC471_001241"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HDF2788379.1"
                     /translation="MSVEVIMPKAGVAMEEGTIVSWLKQEGEEVKIGEPILEITTDKV
                     NMEIESEGEGTLAVIIHKEEGEVLPVFTVIGVIAEKGENQEEVKAKYLSGNVSKEDIV
                     EENQNIEFKEEKINKKECNHDYDVVVIGGGPGGYLSALKAALLGGRVALVEENILGGT
                     CLNRGCIPTKTYIKTAEILEEIDQLSKRGVKVTVDKEQDIKKAIKYKNRVVKKLTAGV
                     GGLLKSRDVDVFNLKASVKEEHKVILSDGKVLDTENIIIATGSKVRILPIKGIESNLI
                     ITSTEALDLETVPEELVIIGGGVIGCEFAEIFNSRGSKVTIVEMEDRVIPRMDKELSE
                     SLKYSLSKKGINVLTKKKVSEFKEEGNNILVCIEGEEPIKADLCLYAIGREANLSGIE
                     DLDIKIDKGSIVVNSKMETSIPSIYAVGDVTGGVMLAHAAFKMGEVAASNALGVNKEV
                     DLGALPSCVYTIPEVASVGITEEDARKKYNVKVGKFNFAGNGRALASGQEQGYVKVVA
                     DAKYGEILGIHMFGCGVAELINHAASFKALEIPTDEASELIFGHPCTSEALMEALADV
                     NGECLHLPKK"
     gene            23157..26105
                     /locus_tag="PC471_001242"
     CDS             23157..26105
                     /locus_tag="PC471_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDF2788380.1"
                     /translation="MRQRIKNLIENEDKKNPLTDEAISSRLNIRREDVTFLRNELKIE
                     DSRQRRKVVLIKAIKDILKEEKTINKNDITRRLNSIGFTVSRFTVIQYLKEIQDSGDL
                     KLDKESDDKKEEIINNRENKDIAFDKLIGSSGSLSTIIKLAKAAILYPPHGLHTIIMG
                     PTGVGKSELAECMYKFAVESSRFPKNAPFIVFNAADYSENPQLLLSQLFGHVKGAFTG
                     ADENKEGLVSKADGGILFIDEIHRLPHEGQEILFQLIDKGMVRKLGETRLTHKIDVMI
                     ISATSEPIDSHLLNTFKRRIPVTIEIPELVARPLNERFDIINNFFLIEAQRMGANIHI
                     KSDVLKALMLYDCIGNVGQLRSDIQVACARGLLNQLTNKLKEVNISISDVPGYVKQGL
                     IKIRNCRGKIENYVDGDLIIDSNLQGEIEKKNEDIYTFPDEIYKLTERRHIDLLEQGL
                     DFDVINRIIGGELESCIQKYIKQVKKVSISNNLPDISPIVGKYIVELTYEVIKIASQY
                     LSSLEPNLHLCLAVHLKAAFERLKEGKVISNAHLKEIIGNYPLEYSVAEKVAQYLKER
                     YNINLPKEEIGIIAIYFKMSSRRDMSEQKKIGVLLMAHGRVASSVCEVTNKLLGINHA
                     RYLDMPFEKKPEEMIEEATTIIKDIDEGRGVIILVDMGSLSYFGDIISGKTGINIRTI
                     SRFDTLLSIEVIRRAVLPDLTIDEIVDEVKGIEPQVKEKVSKCNNTKVDFRKPIILTL
                     CITGLGGACKIKKILEENIKSITEKVDIEPIGMIEYPSLDLEIKQISNDKNVIAIVGT
                     IDPQIANIPFFSLVDINDKAKLNQINNLIDTNQIGETNKKYELSDLIHKEDIFVDLQV
                     KSKEEVLKYMSKKLREKGYVTKNFYKKVVERDNLFPTELSDEIAIPHTDGIDVIRPAI
                     SIAILKKPIVWSKSKVKIILLLAVDQKCFNPLSTLLSFIETDEFKKIKNIKDRDFIRE
                     MILDGVNENN"
     gene            26083..26547
                     /locus_tag="PC471_001243"
     CDS             26083..26547
                     /locus_tag="PC471_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDF2788381.1"
                     /translation="MESMKIISSNLIFKNIEVSNNEDALKFLGQRLFDEQYVKESYIQ
                     AVVAREKKYATGLPTEIYGVAIPHTDIVHVNEPGIAIGILNKPVKFIMMGTDDTEIDV
                     KVVFMLAVKEPQEQLQLLEKLMTIFQDKNILNNIVDLSEESVSDLLNSKLKN"
     gene            26660..26962
                     /locus_tag="PC471_001244"
     CDS             26660..26962
                     /locus_tag="PC471_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDF2788382.1"
                     /translation="MGVTNVAKKILVACGTGVCTSTIAINKLKKALQDIGKLDMVNIT
                     QCKVVEVASKAPDYDLIICTTQVSSSIKTPVINGLPFLTGVGMDKLINDVLEELEL"
     gene            27024..28388
                     /locus_tag="PC471_001245"
     CDS             27024..28388
                     /locus_tag="PC471_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425524.1"
                     /note="with GatAB forms a phosphoenolpyruvate-dependent
                     sugar phosphotransferase transporter for galactitol;
                     subunit IIC forms the translocation channel and contains
                     the substrate binding site; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HDF2788383.1"
                     /translation="MEGLYMEILNFIVGLGAQVMMPIIICIFGLILGTKLGKSLRAGL
                     TVGVGFIGLNTIIALLTDNLGPATQQMVKNLGLSLSIIDVGWPAASAIAFASTVGALI
                     IPIGLVVNIVMLITNTTQTVDVDIWDYWHFAFTGALVAGATQSVMWGVFAAVANMVIV
                     LVMADLTAPGIEEYLGMPGISLPHGFTQAFVPIAIVVNKLLDLIPGINKIEINADTLQ
                     KKFGLFGEPLIMGSVIGVIIGIAAKYDIKGILQLGVTMGAVLILIPKMAALLMEGLLP
                     VSEAAQEFIEKRFKNRGKIYIGLDSAVGIGHPVTLSVALVLVPLTILIAAILPGNKVL
                     PFADLAVIPFALVLIVPITKGNVFRTLIIGIIIITSGLLIATNLAPLFTQMALNASFK
                     MPEGATMISSICDGANPLSWVFVKVMNYKVIGGVVFGVIALGMAIYNRNRIINENKKL
                     SIEE"
     gene            28423..28896
                     /locus_tag="PC471_001246"
     CDS             28423..28896
                     /locus_tag="PC471_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2788384.1"
                     /translation="MKKYKYDSKRYLLSVTIPGFFMILILIYALFNNYTNFNLNIYSL
                     LIIVCTYGIFNTFISSSNPKKIIIDSKCIHFTSFMTTHKYEIKDIEKIRIREFYNKKI
                     YLKINDGKLFKGRYWIRTNMFNNSIALYSYFIELEECLYPDSLKFRNKKFENKNI"
     gene            29068..29718
                     /locus_tag="PC471_001247"
     CDS             29068..29718
                     /locus_tag="PC471_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HDF2788385.1"
                     /translation="MLLEKLRKEVLQASLDLLNYNLVTLTGGNVSGRDEQTGYIAITP
                     SGMDYRNLTPSDIVIVDVDGNIIDGKWKASVDLSDHLYIYKHREDINSIIHTHSTYSS
                     CFAILNEPIECASTTLANEVGGSVPVAKFSPPTSKKMGKCVIEAIGDKRACLLANHGV
                     IAVGPSVGHALTAAVMLEDSAKVYYLAKSIGTPVLLPDEEIQRARDVFFNVYGQDK"
     gene            29892..30824
                     /locus_tag="PC471_001248"
     CDS             29892..30824
                     /locus_tag="PC471_001248"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HDF2788386.1"
                     /translation="MYYLLNKSTNPYFNLALEEYLFLNDKYNDDIIIIWRNEESIFIG
                     KNQNPYQEVYHDVIEKGEIPILRRISGGGTVYHDLGNINMSFIQKDRQLHEIDFLEHT
                     KFMQKMLSTLGLDVSITERKDLFLNGKKISGSAQSIKRKNSLYHGTLLYDSDLNKLTK
                     YLNSNKATESNATKSVSSKVTNIKFLLEKDINYFLDYCVEYLKSNIKNIKELELDEED
                     IKSVYNIGEDKYKKIEWTYGKTPKFQVVLPFNNESKINIHVNRWKVSKFFISYDDNVI
                     NMDKLLDLNFFKEEIKESILENYPHYIDLINLIF"
     gene            31098..31811
                     /gene="ispD"
                     /locus_tag="PC471_001249"
     CDS             31098..31811
                     /gene="ispD"
                     /locus_tag="PC471_001249"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HDF2788387.1"
                     /translation="MYSVIIVAAGSGRRMNLDINKQFIKLREKEIIAHTIQVFYENIN
                     IDEIVVCIKKEEEDFFKENIINKYNFKNIKIAYGGKERQDSIYNGLKKLDKNCDIVLI
                     HDGARPFVDHRIINESIKVAKEKKAVVVGVPVSDTIKIVSDGTVQETPERNLLWAAQT
                     PQTFEYNLIIDAYEQAYKNNYYGTDDSMLVENIGQSVTMVMGSYENIKITSPEDLNIA
                     EQILNMEKRDEVNSRRRII"
     gene            31808..32293
                     /locus_tag="PC471_001250"
     CDS             31808..32293
                     /locus_tag="PC471_001250"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HDF2788388.1"
                     /translation="MRIGLGYDVHKLTEDRKLIIGGVEIPHDKGLLGHSDADVLIHAI
                     MDSILGALALGDIGKHFPDTDEEYKGADSMKLLEHVYNLITSKGYKIGNIDSTIIAQS
                     PKMAPYIESMRSNISKVLNTDIDNINIKATTEEGLGFTGAKQGIASQSICLLLLTSQN
                     N"
     misc_binding    32388..32596
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            32660..34375
                     /locus_tag="PC471_001251"
     CDS             32660..34375
                     /locus_tag="PC471_001251"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HDF2788389.1"
                     /translation="MKMSKMFMPTLKEIPADAEITSHQLMVRSGMIKKMTSGVYNQLP
                     MGLRVFKKIEQIIREELNKKDCQEILCAALLPSELWKESGRWTAMGEEMFRLKDRTER
                     EYCLGPTHEEAFTDIIRQEITSYKQLPLNLYQIQVKYRDERRPRFGVMRTKTFTMKDA
                     YSFDVDDKGLDKSYQDMFDAYVSIFNRCGLENSPVQADSGAIGGSTSAEFMVKSEVGE
                     DEVVFCSGCDYAANVERAESCNLASQKEEMKELEEVHTPGAATIKELEEFLKTSPDKF
                     AKTLVYEADGKTVVVVVRGDREVNEIKVSNAIGSVIEFALATDDVVRKVTNAEVGFAG
                     PIGINADYVFIDKEIVEQRNIVVGANKTEYHIKNANYGRDFEGIVGDFRNVQEGDKCI
                     VCGKPLEIARGVEVGHIFKLGTKYSESMNANFIDKDGKSKPIVMGCYGIGVERTAAAI
                     IEQHNDEKGIIWPLSVAPYHVVIVPANMKNEEQISIAENIYNDLQAMGVEVLLDDRDE
                     RIGVKFNDSELIGIPMRITVGKNINEGKVEFKLRHKEDKEIIDIEEINEKVKAEFIRN
                     NVRLG"
     gene            34497..35942
                     /locus_tag="PC471_001252"
     CDS             34497..35942
                     /locus_tag="PC471_001252"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HDF2788390.1"
                     /translation="MAKNEKQFVEEITKMEDDFPQWYTDVITKTDLVDYAPVKGFMVV
                     KPYGYALWEKMQEFMDKKFKETGHKNCYFPLLIPESLLNKEAEHVEGFAPEVAWVTHG
                     GNKKLEERLCVRPTSETIICTMYAKWLKSYRELPYLYNQWCSVVRWEKSTRPFLRTSE
                     FLWQEGHTLHETAEEAQEETIQQLEVYKALCEELLAMPVVAGQKSESEKFAGGERTYT
                     IEAMMHDGKALQSGTSHFLGQHFTKAFDITFADREGNLANPYHTSWGASTRLIGGLIM
                     THSDNRGLVLPPRVAPIQVVIVPIAAKKGNVMETVDKIYADLKAKGVAVEVDDRDNYT
                     TGWKFNEWEMKGVPVRVEIGPKDIENNQAMVFRRDTLEKDSMPLEGLADAICDLFDVI
                     HNDMFEKARKHREDNTSIVENMDEFRKALEEKPGFIKTMWCGDAECEAKIKEETGATI
                     RCLPFEQENLGHKCVYCGKEADSMVVMAKAY"
     gene            36380..37861
                     /locus_tag="PC471_001253"
     CDS             36380..37861
                     /locus_tag="PC471_001253"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HDF2788391.1"
                     /translation="MNVRVRFAPSPTGFVHIGSLRTALYNYLFAKKMGGEYILRVEDT
                     DQSRLVEGAIENMLNAMKWAGVNHSEGVILDDSGKVVQKGEYGPYIQSQRLDIYQEYI
                     KQLLDSGKAYYCFCTKERLDEVRDAQRAAGETAKYDGHCKNLSKEEVEANIKAGIPYV
                     IRLRLPENHTIKFTDLVRGDMEFNTNDLDDQVLMKTDGFPTYHFAVVVDDYLMKITHV
                     IRGEEWVSSTPKHVYLYEAFGWEAPVFVHLPNILNKEKKKLSKRQGDVAVEDFKKKGY
                     LPEGLVNYVALVGWSPEDNQELFTMEELEKAFSVERVSKSGGVFDTEKLNWVNQHYIK
                     DGDDAYLTDLAIPFLIEDGFITEEEATNKYDFLKSMISVLKEKLQYVKEVTEHASIFF
                     GDKIEVETEEGREFLRLEHIPTLIDALREKIEKTEVLNAEFVQAMLKEIQKEYKIKGK
                     NLFMGSRIILTGQMHGPDLPKVMEVLGKETCLNRIAYVKNNIL"
     gene            38082..38237
                     /locus_tag="PC471_001254"
     CDS             38082..38237
                     /locus_tag="PC471_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2788392.1"
                     /translation="MYSNIDDVKKELKELCLEYVTILEKLKDEKMITEETFEKCSSQK
                     KIFLEEQ"
     gene            38382..39779
                     /locus_tag="PC471_001255"
     CDS             38382..39779
                     /locus_tag="PC471_001255"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HDF2788393.1"
                     /translation="MKVYNTLTRTKEEFVPLEEGKVKMYVCGPTVYNYIHIGNARPFI
                     IFDTLRRYLEYRGYDVTYVQNFTDVDDKIINRSHEEGISPEEVAAKYIKEYFVDCDGL
                     GIKRATVHPQVTDNIQQIIEFIKELEDKGYAYAVNGDVYFDTNKFEGYGKLSGQKQED
                     LEAGARIEVNDQKRHPMDFVLWKAKKEGEPGWDSPWGEGRPGWHIECSVMSKRYLGET
                     IDIHAGGQDLTFPHHENEIAQSEARSGKTFSKYWMHNGYININDEKMSKSKGNFFTVR
                     DISKLYDLEIVRFFMLSAHYRNPVNFSDEMLNQAKAGLERLYNTKEKLEFTLSNLVES
                     PLTEKEVELVKELDDFRQKFIDAMDDDVNTADAVSVIFELAKLINSNVDENSSLEFAK
                     KCLDEFNELTGVLNIVNKKKDTVLDKDIEELIQKRTDAKKNKEFQLADDIRQQLLDMG
                     IVLEDTRQGVKWKRI"
     gene            39764..40177
                     /locus_tag="PC471_001256"
     CDS             39764..40177
                     /locus_tag="PC471_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HDF2788394.1"
                     /translation="MEKDIKMEKTELVTMSPLVLAYLGDTVYETYIREYLIRQNTQRK
                     VNDLHKLAIKYVKAKAQATIIHEVEIELTEEESKIYKRGRNQKSNTSPKNADIIDYKH
                     ATGFEALVGYLYLNNEIERLQYIINKGIKIIERDM"
     gene            40283..41041
                     /locus_tag="PC471_001257"
     CDS             40283..41041
                     /locus_tag="PC471_001257"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HDF2788395.1"
                     /translation="MKVKLISHTPEPEKVIAMAAKLCYSPVGTDEIEKDLTDESIEKF
                     LNMLLSIGHGSILEHASFTFSIEGISRACSHQIVRHRIASFSQQSQRYVKLEQFEYII
                     PPEIEKIEKAKELFIDSMKKDQENYDKLVEILFENHYNDLIKNGKNEKTAKRQAEKKA
                     IEDARYVFPNACETKMVFTINARSLFNFFEHRCCERAQWEIRNLAVEMLREVKKVAPI
                     LFKKTGPSCVNGSCPEGTMTCGDIVQVREKFKAL"
     gene            41054..41791
                     /gene="rlmB"
                     /locus_tag="PC471_001258"
     CDS             41054..41791
                     /gene="rlmB"
                     /locus_tag="PC471_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HDF2788396.1"
                     /translation="MASIEGRNPVIEAIKSDREIDKILIANSAKEGSIKKIIGMAKDK
                     NIIIQYVDKHKLDEVSTSHSHQGVIAYASEYKYYELDELIDLAKNKDEDPFFIILDEI
                     TDPHNLGSIIRTADAVGAHGVIIPKRRSVHITPVVAKASAGAVEYMPVCKVTNIVNTI
                     KRLKEEGLWIAAADMDGETFYKQNLTGPLGVVIGSEGFGISRLVKQNCDFIVKMPMIG
                     NVTSLNASVAGGILLYEIFRQRLDKSK"
     gene            41794..42321
                     /locus_tag="PC471_001259"
     CDS             41794..42321
                     /locus_tag="PC471_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HDF2788397.1"
                     /translation="MKKNINHYLIIDGYNIINAWDNLKELAKEDLEDSREKLIDDIIE
                     FSEFMGYKTIIVFDAYNVKNSREKVEKRKHITIVYTREHQTADSYIEKFITSLSKYDD
                     VKVATNDYAEQQMILGKGATRMSARELKLELDRSKNKMKEKNIGLRKKIQRNWLEERL
                     DKETLSKLENIRRKR"
     gene            42386..43033
                     /gene="sigH"
                     /locus_tag="PC471_001260"
     CDS             42386..43033
                     /gene="sigH"
                     /locus_tag="PC471_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigH"
                     /protein_id="HDF2788398.1"
                     /translation="MLVAKEKSYELVDNCQQDEYNIVLRASEGDKIALEYIITKYRNF
                     VKAKAKSYFLIGADKEDIIQEGMIGLYKAVRDFDGSKTNSFKCFAEICITRQIITAIK
                     TATRQKHIPLNSYVSLNKPIYDEESDRTLLDIIATSIVTDPEELIISKEELKNIESKM
                     NELLSDLELEVLELYLNGKSYQFIADKLKRDVKSIDNALQRVKRKLEKHLENRND"
     gene            43107..>43639
                     /locus_tag="PC471_001261"
     CDS             43107..>43639
                     /locus_tag="PC471_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HDF2788399.1"
                     /translation="MAKAKYERTKPHVNIGTIGHVDHGKTTLTAAITKTLYDRYQLGE
                     AVDFANIDKAPEERERGITISTAHVEYETPNRHYAHVDCPGHADYVKNMITGAAQMDG
                     AILVCSATDGPMPQTREHILLSRQVGVPYIVVFLNKCDMVDDEELLELVEMEVRDLLT
                     EYDFPGDDTPIVRGSAL"
BASE COUNT        17875 a         4542 c         8198 g        13024 t
ORIGIN      
        1 acaaatgttt agtaataaag atgagaaggt atatttaaaa tataaagtat ttaaagttct
       61 gaaatctaat catgaagcaa aaaattagca tttggttatc aataaatata ataaataaaa
      121 actcataatt aatgaaagaa taaattcata tctaaatttc tataaaaaat ttaaatttat
      181 acaatttagg gctttaaaaa tttaaataat aggtgtaaaa taatagtcaa agaaagtcaa
      241 aaataaaaag gtgtgtggtg atattaatgg caactatgac agatataatt gaaaaattta
      301 ttaaagattt gatggaagaa gataatagca tacaaattca aagaaatgaa ctagcaaact
      361 tgtttagttg tgcaccatca caaataaatt atgtcttaac aacaagattt acaatagata
      421 gaggatatta cattgaaagt aaaaaaggtg gcggaggata tgttcaaata gaaaaaatac
      481 gaaaaagtaa agatgggcat attagagaac tactaaatga aaaaatagga agtcagatat
      541 cctataaaaa agcaaaagaa ttattagaag gtttaaaaga gtcggatttg attaacgaaa
      601 gagaattaaa attaatatta tatgcaatag atgataaatc attgtgtatg ccaatatatg
      661 aattaaaaga gaaggtaagg tctaatattt taaagaatat aataatagga ttatttagta
      721 tagaggagtg ataatttatg ctatgtcaaa aatgcaataa gaataaagca tctgtatatt
      781 ataataagat agtcaatgga gaaaaaacag agatgtattt atgtagtgag tgtgcaaaag
      841 agaatacaga aatgaatttt aacttagata tgcctttttc aatgatggat atattttcta
      901 atttaggttt tcaacctaaa aaagaacttg aggaaaagct agtttgtcct aaatgcaata
      961 caacatatag tgagtttaag aataatggga gatttggatg tagtgaatgt tataatgcat
     1021 ttagtagtca agtaaatcca atgcttcaaa atatacatgg gcatatagaa catacaggaa
     1081 aagcacctaa aaaatctttt tataagataa gtgtagaaaa tgagattaaa gaactaaaag
     1141 aagatttaga tagagctata aaaaatgaag agtatgagtt agcagctcaa tttagagata
     1201 aaattaaata tttaaaaggt tctattgact aagattggag tgatattatg aaagagaaca
     1261 tagtcatgaa atcaagagtt aggctagcta gaaatttaaa taactatcca tttccaaata
     1321 aattagataa agaatgtgcc atggaaataa tagaaaaagt aaaaaatgcg tttattaatt
     1381 ctaacttaga acaaaaagaa gaatttgatt tctataaaat tgaagattta gaccaatcta
     1441 aaaaaatgct tatggttgaa gaacatataa taagcccaga tttagcagaa aatgataaaa
     1501 gtgcagttat agtaaaaaaa gataaaacta taagtataat gataaatgaa gaagaccata
     1561 taaggataca aactatatgt gatgatttaa acttagaata tgcatatagt gtagcaaatg
     1621 aaatagatga tttgttagaa tcaagtttag aatatgcctt taacacaaag ctaggttatc
     1681 taacatcttg tccaactaat acaggaacag gaatgagagc ttctgtaatg atgcatttac
     1741 cagcattaag tcaactaggc tatatggatg aactttataa aatttcatca caaataggta
     1801 tagcaattag agggatatat ggagaacgaa cagaagcgtt gggaaatata tatcaaatat
     1861 ctaatcaact tacacttgga agaacagagt ctaatattat agaaaatgta agtggactta
     1921 caaaagatgc aatttcaaaa gaaataaaag ctagagaaat attgcaaaaa aaattaggta
     1981 taaaactaga agataaaata tttagatcga tagggacact tgaaaattca agagtaatga
     2041 gtagcgcaga ggcaatgagc catctatcaa atataaaaat gggaatagaa atgaactata
     2101 tagataaact agatttaagg gctatagaac aacttatgat aggtatacaa ccagcgcatc
     2161 agtcaataat gtataagagt gatgatgttg agaatagaga tataaataga gcaacatata
     2221 ttagagaaac tttggaaaaa ttaagaggag ggatgaattg tgaacttcaa tagatttaca
     2281 caaagagcta aaaaggcaat tgatttagcg tttgaatctg ctaaaagtct aggacacaat
     2341 attgtcggaa gtgaacatat acttttaggt cttttaagag aagaagaagg tatagctgct
     2401 aaagttctaa gtaaagttgg atttacagaa gcttatttag aaggcaaaat agttgatatg
     2461 gaaggtaagg gagaagaaat ctcagaagat atagtattaa gtccaaggag taaacaaata
     2521 cttgaattat caggaatgtt tgcaaataaa ttaaaaacaa attatattgg gactgagcat
     2581 atattattag caattattca agaaggtgaa ggaatagcta ataagatttt aaattatgct
     2641 ggagtaaatg ataggacttt agcccaatta acaattgata tgatgggtat tagtgataaa
     2701 aatcaataca aagcagagaa tagttacacg ggcaaccaga atcaagcaga atctaaagta
     2761 cttgataagt atggaagaaa tcttacacta tatgcaaaac agaataaaat agaccctgta
     2821 ataggtagag aaaaagaaat acaaagagta atacaaatat taagtagaag aactaaaaac
     2881 aatccagtgt tgataggaga cccaggtgta gggaaaacag ccatagctga aggtctggct
     2941 acaaatatag ccttaggaaa tgttccagaa acactaaaaa acaaaacttt atactcttta
     3001 gagatgggtt cattattggc tggagctaag tatagaggag aatttgaaga aagaattaaa
     3061 gaagttgtag atgaagttgt taaaaatgga aacataatct tatttataga tgagatgcac
     3121 acaataatag gggctgggtc tactggagaa ggctctatag atgcttcaaa tatattaaag
     3181 ccagccttag caagaggaga aatacaagtt ataggtgcaa ctacaattga tgaatataga
     3241 aagcatgttg aaaaagattc tgctctagaa agaagattcc aaccagttat ggtagatgaa
     3301 ccaactaaag aggactcaat aaaaatatta gaaggattaa gagataaata tgaagctcat
     3361 cataaagtta aaataactga tgatgctata aaggcagctg tagaattatc aactagatat
     3421 atatcagaca gatatctgcc agataaagct atagacttga ttgatgaagc tgcatcaaaa
     3481 gtaagattaa aagaaaatac acctccttca gaaataaaaa aattagaact agaaatagaa
     3541 aatatagata aagaaaaaga agaagcagta agatgtcaag actttgagaa agcagccaaa
     3601 ataagagatg aacaaggtct acttaaaaaa caattagaag atgttagaga aagatggaac
     3661 aagtcatcta aacattcaga tttagttgat ggagaagtta ttgctgaagt agtgggctta
     3721 tggacaggaa taccagttaa taaaattctt gaagaagagg ctgatagact tttgagactt
     3781 gaggaaatac tgcacaataa agttataggt caagaacaag cagtaaaatc tatttcaaag
     3841 gcaatcagaa ggtcaagagc aggtcttaaa gaccctaata gaccaattgg ttcattttta
     3901 tttttaggtc caacaggagt aggtaaaaca gaattatcta aggcattagc agaagtgcaa
     3961 tttggagatg aaaatcaaat aattagaata gatatgtctg aatatatgga aaaacatgct
     4021 gtgtcaagaa tgataggttc acctccaggt tatgtaggtc atgatgaagg tggtcaatta
     4081 actgaaaaag taagaagaaa tccatattca gttatcttat ttgatgagat agaaaaagct
     4141 catccagatg tatttaatat tctgttacaa attctagatg atggtagact aactgattca
     4201 aaaggaagaa ctgtagattt taagaatact attgtaataa tgacatcaaa tgttggagca
     4261 tctacaattg gtagacaaaa aactttagga tttagtatag ctaaaggaga cgaagaagaa
     4321 aaatctcaat atgaaaaaat gaaagaaaat ataatgggtg aattaaaaca aagattcaga
     4381 ccagagtttt taaacagaat agatgatata attgttttcc actcactaaa tgaaaatcat
     4441 atatcaaaaa tagtactttt aatggcagcg aaactacaag agagattaaa agaaatggat
     4501 ataaaattag aaatgagtga tgaggctgtt aaattaattt ctaaatctgg atttgactta
     4561 gaatatggag caagacctct taaaagagct ttacaaaaag agttggaaga tgagctatct
     4621 gaagcaatct taaaaggtaa tgtgaaaaag ggaagtaatg tagtagcaaa agtcaaagat
     4681 gaaaaaatag tttttgaaac taaataattt aatagagttg cattttaaaa tattgcaaat
     4741 ataagtttta taatctaaac aaaattttaa gggcaatgaa tctataataa ttcattgtcc
     4801 ttaattattt tatttaatct ttacatattg tctttcagat atgctaaaat tatagtatag
     4861 aataaaaaat taaggatgat aaaagtatgg caaaaataaa aacaaaatat gtatgtcaat
     4921 cttgcggata tgaaacagct aaatggcttg gtaaatgtcc agaatgtaca aaatggaata
     4981 catttgtaga agaaatagac caaaaaagca ccaaaaaaga ggtctttata atagataaat
     5041 cttcatctaa accagtcagt ataaactcaa tagagagtaa agaagaagag cggtttacaa
     5101 cagatataaa tgaattagat agagtgcttg gaggaggtat agttaaaggc tctttagtgc
     5161 ttgttggtgg tgaccctggt ataggtaaat caacactttt aatccaagta tctagtaatg
     5221 ttgccaattt aggaaaaact gttttatata taactggaga agaatcagaa tctcaaataa
     5281 aaatgagagc taaaagatta ggtattaact cagaaaaatt atatatattt gctgaaaata
     5341 atttaagtat aattgaatct tatttagaaa gtgtaaatcc agaattaata attctagact
     5401 caattcaaac tgtcttcagt ccagaaatat cctcggcacc aggaactgtc agccaaatta
     5461 aagaaggtac ttcaaaattc atgaaaattt ctaaaaaaat gggaatttca acgttcatag
     5521 ttgggcatgt tacaaaagaa ggttcactag caggaccaaa attattagag catatggttg
     5581 atactgttct atattttgaa ggagaaagat ataatactta tagattagta agagctgtta
     5641 aaaatcgatt tggttcaact aatgaacttg gagtatttga aatgagggat ttaggattag
     5701 tagaacttga taatccatct aaaattttaa tctctgaaaa accaaaagat gtagcaggtt
     5761 cagtaataat atctactgta gaaggtacga ggccaatgtt acttgaattg caagcattag
     5821 tttctcctac aagttttggt ataccaaaaa gaacatctac aggagtcgat tacaatagag
     5881 taggtatgtt attagcagta ttagaaaaac gtgttggttt gcaaatacaa aatcaagatg
     5941 tctatataaa catagttgga ggaataaaga taaatgaacc ctcaattgat ttagggatag
     6001 ccatttctgt agcatctagt tttagaaata taccgataga tgaagatata gctgttacag
     6061 gagaagtggg acttacagga gaagttagag cagtaagctt tatagaaaag agaatagctg
     6121 agtgtaaaaa acttggattt aaaaagatag tagttcctag aagtaattat gatgttgtaa
     6181 aagagaccaa aggcatagaa atatggccag tagataactt aagacaagct ataaatatag
     6241 tacttgggag gaatcaataa atatggagaa ttttctagat aataaaaata tgctatatgc
     6301 attaaaaatg atatctcctg gaactccact tagattaggt ctaaacaatg tactaagagc
     6361 taagactggt ggattaattg taattgcaac aaacgaagat gtaatgaaaa tagtagatgg
     6421 aggatttgct ataaatgcag aatattcacc atcatatcta tatgaattag ctaaaatgga
     6481 tggagctata gttttaagtg gtgatgtaaa gaaaatatta tttgctaatg cacaacttat
     6541 acctgactat tttatagaaa catcagagac aggaacaaga catagaacag cagaaagagt
     6601 agcaaaacaa actggtgcta tagtcatagg aatttcacaa agaagaaatg ttataacagt
     6661 ttatagagga aatgagaagt atgtagtcga agatatatct aagatattta ctaaggcaaa
     6721 tcaggctata caaactctgg aaaaatataa gacagtattg gaccaagctg taacaaattt
     6781 aaatgcctta gagtttaatg atttggtaac tatttatgat gttgcattag tcatgcaaaa
     6841 gatggaaatg gtaatgagag ttacaagtat aattgaaaaa tatgtgatag aattgggtga
     6901 tgaaggaact ttagtaagta tgcaattaga agaattaatg ggtacaacca gaatagacca
     6961 gaaattaata ttcaaagatt ataataaaga aaacacagaa ataaaagaac ttatgaaaaa
     7021 ggtcaaaaat ttaaattcag aagaactaat agaattggtc aatatggcaa aactattagg
     7081 gtatagtggt ttttcagaaa gtatggatat gcctataaaa acaagaggtt ataggattct
     7141 tagcaaaata catagactac caacagcaat aatagaaaac ttagtaaatt attttgaaaa
     7201 ctttcaacaa attttagatg catctattga agaattagat gaggttgaag gaataggtga
     7261 aataagagca acatatataa aaaatggact cataaaaatg aaacaattag tcttattaga
     7321 tagacacata tgatgaattt taaatgaatt ttactacttt tttcttgaat ataatataca
     7381 ctttggtcta taatagataa gtataactag ggggtgaagc attgataagg aaagtaacaa
     7441 gaatattgtt tattttactt ggatttacaa taggtataac aacctattta acattaatga
     7501 aagattttga aatattaaca tttggaaaag aaacatatgg ttatatcgca gctgtagtag
     7561 caggtataat aatagccatt ttaggatatc ttatagagcc atgggttgta aataaagtaa
     7621 aagaaattgc taaaatagtg gataaagaat tgtcaaaata tcctcagact gatatattgt
     7681 tagggtctat gggtcttata gtaggatttg ttattgcgta tttattaagt ggattagtaa
     7741 atagaatacc aatagtaggt ggaatactat ccttactatt atatttattc ctaggatatc
     7801 ttggaatgaa agttgcgctt aaaagtaaaa atgatttatt taatattgga aaactaggaa
     7861 gattagcaaa tcctattaag gataaagata aggataaaga aaataaaaaa gaagtaaaag
     7921 ccattcctcc aaaagtatta gatacaagtg taataattga tggaagaata gctgatattt
     7981 gtaagactgg gtttatagaa ggaaaactta ttattcctgc ttttgttttg gaggaactaa
     8041 gacatattgc agattcttca gatgatttaa aaagagtaag aggtagaaga ggtctggata
     8101 tattaaatat aattcagaaa gaattaaata tagaagttga aataagtgag agagactttg
     8161 atgatatagc agaagttgat agtaaattat taaagcttgc tcaggtttta aatggaaaag
     8221 ttgttacaaa tgattataac ttaaataaag ttgctcaatt ccaaggtgtt gaagtattaa
     8281 atataaatga gttagctaat gctataaaac cagttgctat accaggtgaa gatatggttg
     8341 ttcaagtggt taaagaagga aaagaggctc aacaaggtgt tgcatatcta gatgatggaa
     8401 ctatgatagt tgttgatgga ggaagaaaat atatgaatga gactataaaa gtactcgtta
     8461 cttctgttct tcaaactcct gcaggtagaa tgatatttgc aaaacctaaa aattaatatg
     8521 ttcaaagaaa cctataattt gggctgttta ttttaaatag atggtccaaa tttatttatt
     8581 tataacgata atgtttaatg tattcttaaa gatggggatt taaatcagaa agcagggaga
     8641 atttatgaga aaaaagatta tatctttctt agcaacattt atcataatat taacatcagc
     8701 aagtcagtat agttttgcag atgatatatc aacacgtgga aaagtaatat ttatagatat
     8761 gaatagaact agtatgagta atatgttaag aataaaatca ttaagagaag aactagacaa
     8821 tagagggtat attggcttga tgaatataag aggcgataaa ggaagtgatg atagaaggtc
     8881 atatgctagt atgggtgctg gtggtagagc taatgtggct aatgaagaag atattaattt
     8941 tgaaagctcg agtaaggata gaaatatagt ttttgaatca gctacaggaa aaagtgcaaa
     9001 aggtataaat aatttaacta taaataagtc tataaatgaa aatttaaact ttggagagta
     9061 tggttctgta ttagggtcat taggacaaag tttatctgat aatggattaa aagcatctgt
     9121 attgggtaat tcagatataa ttgaaaatgg tcaacttata aaaaatagaa acttatgtct
     9181 aactgcaatg gatgaatatg gaagaatacc taatggaaat gtagatacta taaataaaaa
     9241 agacttatct atgccttatg gaatatcaac tgattatgat aaattgattg tagagactaa
     9301 agaagcttac aaaaataatg atgtgatttt tgtggagctt ggagacacat atagattaga
     9361 tttatataaa ccaaatttaa atgaaaaaac atatgagagt atgaaagata acatagaaaa
     9421 aaatatagat atatatttaa aaaatatatt ttctatggtt gaagaaaatg acactgtcta
     9481 catagcaagt gcatttccaa gtgatttaga ttataaaaat aaaagaagac tttcgccaat
     9541 aataaaatta aatggagaag ggaaaggtat attatcatct tcaactacta gaagagaagg
     9601 tattgtaaca aatttagatg ttggggttga aatattagac aactttaata taaaaaatca
     9661 aaatatggtt ggaagaaaat atgaacttat taacagagat gataataaag aatttttgat
     9721 ggatgaatat caaaagatag tatctatttc aagcataagg tctacaatac tgaatggttt
     9781 tgtaagtata gttttcttgt catggatagt tgctatgatt gcaattttat ttagaaagca
     9841 tatatcaaaa aattataaag aaacaacatt ttttatatta aaggaacttt taaaaatagg
     9901 aatagtaatg ccactgtcat ttatgataac accaataatg aatttcaaaa caccattagc
     9961 aatatcttta ggaataatta taataacact tactatatat cttataagta aagttttaat
    10021 aaagaacgat ttaaaaaaca tgctgttttt tactggatta accatagtaa ttatggttat
    10081 tgatgctggg tttggctcat atttaatgaa aagcaatgtt atgagttatg attgtataat
    10141 tggagctaga tattatggag taggaaatga gtatcaaggt gtggcaatcg gtagtgcaat
    10201 atttacattt gcaattttac ttacatataa aaatatacca aaatggtcta taatagtttt
    10261 ttctttggtt atcttaatta caagtgcatc tccaattatg ggagcaaatg ttggaagtgc
    10321 tatttctgaa tgtgttgcat ttttactatt tatactattg atatataatg taaagataga
    10381 tttcaaaaaa atcgtattat taggtatagc agtagtattt gtattaggag tttttgtagc
    10441 aatagatatg attttgggaa gtaattcaca tttaggcatg tttgtaaaag aaatttactt
    10501 taatggacca ggagaaataa tacaaacatt tagtagaaag atagagatga atttaaagct
    10561 agctcaaacc agtgcatggg taaatatact gttaactgga ataggtgtaa ttttagttct
    10621 tatgattaat cagattagat actttaagca actgatggat gagtatccaa ttgtatttaa
    10681 aggatttata gcatcaatag caggatgttt agtaactctt ttagtgaatg actctgggat
    10741 tgtgtcatca gctacagcct ttatttatgt aattgtacct atgataacat taagtataaa
    10801 tcttacagca ttaaaagaat aagtactgta tgaataatat gtaaatatta atacattaat
    10861 ttaacttctt tatttacaaa ataaagaaga aagtttataa aaaataaatt ataataaaaa
    10921 agagtttatc tcaaaattga ttattctaat caaattttaa gataaactct tttttattat
    10981 aaagataaat aacatataac ttcatatttt aggtagattt tttcttatat gtttaggagt
    11041 gcatttaaat ttttccttaa aaattctgta aaataatttt gtattagtaa atccatgagc
    11101 ttctaatata ctgcataagg tatcatttgt attaattaag tctttataaa tatgagaaat
    11161 acgcacatca tttaaatact ctaagtaagt ttgtcccata tactgtttaa attttctaca
    11221 aaaatattct ggttgtaagt gtgctacctt agcgattttg ttaatagaaa taggcatttt
    11281 gtaattaatc tttgtatatt ctaaaactgg ttcaaatttt gacaaatcaa gtactgtttt
    11341 tttcatattt ttattaccta cagatatttt aaagttagta tataattcaa ataaaagttc
    11401 aaataataag cttgtaaaga gcagattagc tgcaggagga gaactaagtt taatttctct
    11461 catgttttct aatataagtt taactttact tgaatttttt ttatagttat tgtcattggc
    11521 attaacctta aagtcaaaat agcaattttt aaaatcaggg atatatttgt ttagaaaaga
    11581 caatggtact tgcaataata tagaggtatt ttgataaata catctagttg agtgaataat
    11641 tccagaatta atgaacatac actcattagc ttttaaatta taagtgtaac cctccatgtt
    11701 aatttgtaaa tcaccagaag ttatataaat tatttctaat gagttatgcc aatgatttgc
    11761 tacataacta ccactatcat caaatagttt gaaattaaca tctaaattat caataggttc
    11821 aattatttca tgtgaaaaat tatttctgtt cataataata tcctccttat aattttaaat
    11881 tataaatagt aaatttataa tgttcaatat taaaaagata atatagatta ataaattaga
    11941 taataaacat acttaagaaa acggataaaa tcaaatataa taactgtata ttaaaataac
    12001 ttaaaaaaat tattttatta taagtgcaaa atatacctat ttaaataaga taagagggga
    12061 ggattttatg tgtatataaa aggtgtaaat ttaggaggtt ggttagtttt agaaaaatgg
    12121 atgacctcaa gtttatttga aggcacagaa gctgaagatg aatactattt accaagacag
    12181 ctttcaagag aggcatatga atctagaatt aagactcatc gtagtgaata catcacagag
    12241 agagattttg caatgattaa atctatggga tttaattcag ttcgtatacc agtaccatac
    12301 tttatttttg gagattgtga accattcata ggatgtgtta aagaacttga taaagcattt
    12361 gcttgggcag ataagtatgg gcttagtata ttgatagact tacatacagt tccaggaagc
    12421 caaaatggat ttgataatgg aggaatatca ggtatatgta gctggtcaca aaatccagaa
    12481 tatgtagcgt ttacattaaa tgtattagaa agacttgcaa aacgttatgg gatgaggcat
    12541 gaattgtatg gaatccaaat tttaaatgag cctataacag aaagaatgtg gaacataatg
    12601 aatgttccaa atcggtttaa ggctgtagat aaggagatgg caagggggag taaacctaat
    12661 tcattagagt ttttacgtga tttttatata aaagcatata aggtaatgcg tccttatatg
    12721 agggaggaaa acgttatagt atttcatgat gcatttgaat taaaagcatg gaaagacttt
    12781 atgagagagg aagaatttaa aaatgttgtt ctagatacac accaatattt gatgttagct
    12841 gaagcagatg gatgtgaaca aagtattgat agctatctca aatatattag ggaaaattat
    12901 gctaaagata ttttacaaat gcaaaaatat tttcctgtta tatgtgggga gtggagctta
    12961 tttaattcat atgcttgtgg tatagataca aatggaggtc aatcacctct aaatgggatt
    13021 gaatcaaata tagataaatt atcaaaagac gataaaagag aattatatag gaagatagca
    13081 aaagcacagc ttgatgcatg gagaaatgga agtggtcact actattggaa ttataagtta
    13141 ttactagata cagttaatga agaaggttgg ataggatggg atagttggga cctagtcaaa
    13201 tgtgtagctc aagaatggta tcctatagaa tattaaacta aaattggagg aaaagatatg
    13261 attaaaaacc ctatacttcc aggttttaat ccagatcctt gtatttgtag aaagggagat
    13321 gattattatc ttgtagtttc atcttttgaa tggtttccag gtatacctgt ttatcactct
    13381 aaagacttaa aaaattggga gttatatact catattttga cagatgaaac aaaaatagat
    13441 ttgaagaagc ttccttcatc taaaggaatt tgggcaccct gtttgactta ttgtgaagaa
    13501 gaagatttat tttatatagt ttatggaatt atgaactcta tgaatgcaag atattttgat
    13561 gttgataatt atttaattac atcaaaagat ataaagggag agtggagtga accagtatat
    13621 ctacactcat caggttttga tgcgtctatt tttcatgatg atgatggtaa aaagtggatt
    13681 gcatcattag actgggagac aagagaagga tatgaaaaac ctggtgttat atgtttagtg
    13741 gaatactgta caaaaaagaa agaaatagta ggatatccaa agagaatatg gtcaggggga
    13801 actgatagag gttgtattga agcaccacat ataacaaagc gtggagatta ctattatata
    13861 atgtgtgcag aaggtggaac tggatatggt catagtgtaa ctatgggaag ggctaaaaat
    13921 atatggggac cttatgaaaa agattcaatg aatccaattg taacttcaat tccaggcgat
    13981 ttttatgaaa gacatgaccc tgaccatcta aagcctaaat actataatcc agaatcaaaa
    14041 cttcaaaaat ctggtcatgg aagctatata gaaacaacat caggtgaagt atatttagtt
    14101 catctaacat ctagaccatt tgtaccagag ttaaggtgta ctttaggtag agaaacagct
    14161 attcaaaaaa tgaaatggac aaaggataat tggcttcgaa tggaagatga aagtaatttg
    14221 gcaaaagaat acgtatcaga aagtaaacta gaagaacatt tagtttcatc tataccaagt
    14281 tttgatgact ttgattcaaa tgaattaggt ttacagtact atgctcctag gatttcacca
    14341 ttatcatttg cagatgttaa gtctagacca ggatatgtaa ggattagagg acaagaatca
    14401 agaacatcat taaataaagt gagcatattg gcaagaaaat taacttcagt atatgcaaga
    14461 ataaccacta agatggagtt ttacccagag gtacatcagc atagtgcggg tttgataatg
    14521 tattatgata atatgaatta tataaattta agaaagtatt atagtgaaac attgggtcaa
    14581 agtgcactat caataataca tcttgaaaat ggagagaaaa cggaattttt aaatactagg
    14641 ataccaataa aagatatacc aatatatctt cgtctgtatg tacaaggtcg taagtcatat
    14701 tttgaatgga gttatgatga aaaaaactac cagagaatag gaaaggtatt tgatacaact
    14761 aagttttcag atgagtactg taaatatgga gaatttacag gtacgtttat aggattaact
    14821 tgtgcagatc gtgtaaagca taaacactat gctgattttg atttttttga atatatagta
    14881 gatgaatcaa aagatgtcga ttaataaaat cattactaaa attttcagag caaaggtaaa
    14941 tcttatgctc tgaaataaga aacattagaa ggcaaatctt acaaaaagga gtatggtatt
    15001 atgaatacag aagttaatgt taaagcaact acgattagat atgaaaagat ttcatttttt
    15061 gaaagaattt cttatggttg tggggatttg gggtgcaata tcatatactc agctatgtca
    15121 gcatttttgc tgttttatta tactaattat gcagatgtta gtgcagcagc tgtaggtagt
    15181 attatgcttg tatctaggat actggatggg tttagtgact taacaatggg aattatagtg
    15241 gatagaacta aatccaaata cggtaaggct aggccttgga ttttgagaat ggcgattcct
    15301 tttgctatag cagcagtatt attattttct gttccatcaa atcttggtat cacttctaaa
    15361 ttaatttata tttttattac atataactta gtttctactg ttatttatac agcaatcaat
    15421 gtaccctatg caactctaaa ttcacttata actcaaaacc agtatgaaag aggagtatta
    15481 agtatattta gaatgatatt agccacttgt ggtaccttac taataaatgg acttacacta
    15541 cctctagttg aatactttgg aaataattta tcagcatgga ctaaaacatt ctttgtattt
    15601 ggaatagcat ctataatggt attttttata acatttactg gaaccaagga aagagtaaaa
    15661 gcagttaagc aagataggaa tgaagtaata ccatttaaaa taggtattaa atctttattt
    15721 agaaataagt actggataca aataacatta tgtttagttt gcatatttat tgtatttgcg
    15781 ataaatggtg gttcatccgt atattatgca aagtttattt tgggagatga aaaattattt
    15841 ggtcctataa atatggtttc aaatatatcc caaattatag ctatgtttat ggttgctcct
    15901 tttataaaaa aatttggaaa aagaaatgta ttaatagtag gttcaataat attaatatcg
    15961 tcaaatgtta tgtttataat agttggacaa aatcatatag gagtaatatt tgcaagtgct
    16021 ataaagggaa taggaagtgc tggaatagca tcaacaatgt ttgctattgt ttcagatact
    16081 attgaatatg gagaatggaa gactggatat agaactgagg ggcttataaa tagtgcatct
    16141 agttttgggt ttaaagtggg aaatggatta ggttcagcaa tattaggtgc agtattatca
    16201 attggtggtt atgtaggaac atcagctact caaagtgatt tagctatagt atcaataaaa
    16261 gtatgtttta tatacctacc tatatttata acaatattac aaattatcat aatgtctttt
    16321 tataagcttg ataaagaata tagtactata cttaatgaat taaatactaa ataatgaaaa
    16381 agtttcttat aaagtcttaa gtttaattaa ggtctattgt aaatgttccc tataaaaact
    16441 attatatatt tatgattatt gaagaatttt tagagaagcg aataataaaa atataagaag
    16501 actttaaatg atgaaatggt tggtttttta gaagtgattt aataagttat tgttatttaa
    16561 agcattataa gtggacaatt aatttttaat ttatggagat gatatttaac aattagaagt
    16621 aatatataat atgcactaag aagcaatttt tcaactattt atgaaggtgg tggtacaaga
    16681 aaagtatcac cttttttttg ttatacaaaa aaacagttga ctttttatat gaaccccttc
    16741 tataatgtta ctgaaattaa gtaaaatgat tgaacaaatg taaagataat tgaaaagctt
    16801 gattttggag gaaagcaaat gaaaaaaatt ggagacttaa gattaatgat gaagtgttgt
    16861 tcactttatt acgaagataa tttgaatcaa caggaaatag caaatcaatt gggaatatca
    16921 agaccaacaa tatcaagaat attaaaagaa gcttttgaac aaggaatagt aaaaatacag
    16981 atagttgatg ttttgaaaaa tgattaccaa aaaatagaga gaagtttgga gagaaaatat
    17041 aaattaaaag aagtaatagt agtagatgat aaacaggatg cactaacaca aaaacaagaa
    17101 ctagcaagag ctgtatctga atatttaact agagtagtaa aggaaaatga tataataggt
    17161 gtttcaatag gaacaacact aaaagaaata ccaagatatg tggaaaaaag taactgtaaa
    17221 aatgttacat ttatacctct attaggaggg attggagaca atgaaataga tattcatgca
    17281 aatcaaatag ccgtaagttt agcgagggct tttggaggtg attttaaatt attacatgct
    17341 ccagctgtta tgtctgactt gtctacaaaa gagaaacttt gcaaagacga aaaaattaaa
    17401 gaagtactag atttaataga taaaacaact atagcaattg tgggtatagg aaatcctatg
    17461 agtttaaact caacaataat ggcttctgga tacatgaatg aagcagatat agaagattta
    17521 aaaaaataca actcaatagg tgcaatttgt ttacaagcct ttgataaaga aggaaaaact
    17581 tctatacttg aatttaatca aagagtatta ggagtgaaac tggaagattt aaaaaagata
    17641 aaaagaacaa taggtgtcgc ttcaggagat gaaaaaatag aggcaataaa ggcatcatta
    17701 aaagcgaaat ttataaatag tttagcaatt aatcacagtt tagcacttaa attattagaa
    17761 gattgtgact aatagaaaga tagataaaag ttaacaggag ggaatgtata tgtcaatatc
    17821 aaaagaaaca ttgcttgaaa tgtataagag aatgaatcag gctagaaaat ttgaagagaa
    17881 agttagttgg ttttttgcaa gaggtatggt tcatggaaca actcacttat ctgtaggtca
    17941 agaagcgtca agtgtggcgg ctgtaatggc tttagaaaaa ggagatttag tatcattaac
    18001 tcatagagga catagtcaat ttataggaat gggaatagac ctaaataaaa tgatggctga
    18061 acttatgggt aaagaaactg gattttgtaa gggaaaagga ggctcaatgc atatagcaga
    18121 tattgagtca ggtaacttag gagcaaatgg agttgtaggt ggaggtctta caatagctcc
    18181 aggagctgca cttacacaac agtataaaaa aacaggcaaa atagttttat gtagttttgg
    18241 tgatggagca tcaaatgaag gtacttttca tgaaggaata aatttatctt caatatggaa
    18301 attgccaata atattttact gtgagaataa tttatacggt atgtcaacaa gcataaaacg
    18361 tcatatgaat atagaaagta ttgccactag agcagcttct tatggaatag aaggaatatc
    18421 aatagatgga tataacccaa tagaagtata cgaaacagta caaaaagctg ctgagaagtg
    18481 tagaagagga gaaggacctg tattaattga aagtagaact tatagatggt taggtcattc
    18541 aaaatcagat gcaaatgtat atagaacaaa agaagaaata gaatcatgga aagcaaaaga
    18601 ccctattgaa tttttaaaaa attatcttat agaaaataat ctatctaatg aagatgaatt
    18661 agataagatt caagaatttg caaagcaatc aatagaagat gcagtagagt ttgctcaaaa
    18721 tagcccaaat ccaaaaatag agtctttatt agaggatgta tatgcagact aagaatggta
    18781 aaggaggaaa ataaaaatga gtacaaggga attaacatat gcacaagcaa taaaggaagc
    18841 aatgtcagaa gaaatgagaa gagatgaaaa tgtaatattt atgggagaag atataggaat
    18901 atatggagga gcatttggtg tatcagttgg tatgatagat gaatttggac cagaaagagt
    18961 aagggatact cctatatcag aagcagctat agcaggagcg gcagcaggtg cagcagcaac
    19021 tggcttaaga cctattatgg aagtaatgtt tatggacttt gtaacaattt ctatggatgc
    19081 aattgtaaat caagcggcaa aaatgagata tatgtttgga ggaaaagcac aagttccaat
    19141 ggttgttaga tgcccaggag gttctggaac tggttcagca gagcaacatt cacaaagttt
    19201 agaagcttgg ttttgtcatg taccaggagt aaaagtagtt gcaccttcta caccagcaga
    19261 tgctaaggga ttgttgaaag cagctataag agataataat ccagtcatat ttgttgagaa
    19321 taaattgtta tacagaaaaa aaggttttgt gccagaagat gactatgtaa ttgaaattgg
    19381 aaaggcagat attaaaagag agggtactga tgttacagta attacttatg gaagaatgtt
    19441 acaaagtgtt gaagaagcag cagagactct atcaaaagaa aatataaatg ttgaaattat
    19501 agatttaaga actttatatc cattagataa agagactata gtaaagagtg tatgtaaaac
    19561 tgggagagtt ttaatttgcc atgaagcagc taagactggt gggctaggtg gagaaatatc
    19621 agcactgata acagaaagtg aatcatttga ttatttagat gctccagtaa aaagaatttg
    19681 tggaaaagat gtgccaatac catataatcc agagttagaa aaagcagttg tcccaagagt
    19741 tgatgaaata gaagaagcaa taaaatcttt aattgttaga taaggaggga tagatatggt
    19801 ggcaaataaa ataaaagcca caccagcagc tagaagtcag gcaagaaaag acaatataaa
    19861 attagatagg ctgattggaa gtggagaaaa tggaaggatt catttagtag atgtgttaaa
    19921 ttatttaaaa gataataaag caaatactac tcctttagct agaagaatag cagaagattt
    19981 aaatatagat ttagaaacta ttgtaggtac tggatataat ggaaagataa gaaagtgtga
    20041 tgtggaaaaa ttaactgcaa aagaaactat tgtatcgact aatacatcta aatctagtga
    20101 gaaaaaagag ctgaaaattg aaaatgaaaa ttcaagcatg tttaacacag tagaaggaat
    20161 atttgaaaag ccaaatccaa tgagagctac tgtagcaaaa cgaatgtcag aaagttattt
    20221 tagtgctcct gtatttacat tcaatataga agtagatgct acagaattga aggtacttag
    20281 agcaaagtta atcgacacag taaaagaatc aactggagta aaattaacta tgacagattt
    20341 aatagtaatg gcagtatcta aaatacttcc aaatcatcaa gccttaaatt cagcatggac
    20401 agatgagggt atatttagat ataaagatgt aaatatagct attgcagttg gtttagatga
    20461 aggcttatat gttccggtag taaaaaatgc aaacaaaaag tctttaaaag aaatagcaaa
    20521 agaaagcaaa gaacttgcgg aaaaagtaaa gacagggaaa cttatgccag cagaccaaga
    20581 aggtaataca tttacaataa gtaatgttgg aatgtatggt ataactacat ttacacctat
    20641 aataaatatg ccttcaagtg caatcttagg ggtaggtgct acacaagata agtttgttcc
    20701 agtaaatgga gaggctaaaa taaaacctat aatgaactta tcattaacat cagaccatag
    20761 agtaatagat ggaacagtag ctgcaaaatt cttaaaagat ttaaaagaat tgttagaaaa
    20821 tcctttatca atgcttgtat aggttttgat attttgttac tgatataaat agtttgaata
    20881 gattatatta ttataattgt ttcagtaaat ttaaagcttt tatatattaa gaattatttt
    20941 taacagtaac tatatgtgaa tatgatttgc ttaattaaaa tataagaaaa taaataggag
    21001 gagagcctat gtcagtagaa gtaataatgc caaaagcagg agtagccatg gaagaaggta
    21061 ctatagtatc ttggctaaag caagaaggcg aagaagtaaa gattggagaa cctatactag
    21121 aaataacaac agataaagtt aatatggaga tagagtcaga aggagaaggt acacttgctg
    21181 taatcataca taaagaggaa ggtgaagtat taccagtatt tacagtaata ggtgtaatag
    21241 cagaaaaagg ggaaaatcaa gaggaagtaa aagctaaata tttatctgga aatgtttcaa
    21301 aagaagatat agttgaagag aatcaaaata tagaatttaa agaagaaaaa ataaacaaaa
    21361 aagagtgtaa tcatgattat gatgtagtgg taataggagg aggacctggg ggatatttat
    21421 cagcattaaa agcagctctt ttaggtggaa gagttgcttt agttgaagaa aatatactag
    21481 gaggtacttg cttaaataga ggttgtatcc ctacaaagac ttatataaaa acagcagaaa
    21541 tattagaaga aattgaccaa ttatcaaaga gaggtgtaaa agtaactgta gataaagaac
    21601 aggacattaa gaaggccata aaatataaaa atagagtagt taaaaaactt acagctggtg
    21661 ttggcggatt attaaaaagt agagatgtag atgtgtttaa tcttaaagct agtgttaaag
    21721 aagagcataa agtaatttta tcagatggaa aagttttaga tacagaaaat ataataattg
    21781 ctacaggttc caaagtaagg atattaccta taaaaggaat agaatcaaat ttaataataa
    21841 ctagtacaga agcattagat ttggaaacag tgccagaaga attagtaata attggtggtg
    21901 gagtgattgg ttgtgaattt gcagagatat tcaattcaag aggttctaaa gtaactatag
    21961 tagaaatgga agatagagta atcccaagaa tggataaaga gttaagcgaa tctttaaaat
    22021 attctcttag taaaaaaggt ataaatgtat taactaaaaa gaaagtttct gaatttaaag
    22081 aagaaggaaa taacatatta gtgtgcattg aaggtgaaga gccaataaaa gcagatttat
    22141 gtctatatgc tataggaaga gaagctaatc tttctggcat agaggactta gatattaaaa
    22201 tagataaagg ttcaatagtt gtaaatagta aaatggaaac aagtatacca agcatatatg
    22261 cagtaggtga tgttactgga ggtgtaatgt tagctcatgc tgcttttaaa atgggagagg
    22321 tagcagcttc aaatgctcta ggtgtgaata aagaagttga cttaggtgca ttacctagtt
    22381 gtgtttatac aattccagaa gtagcatcag ttggtattac agaagaggat gctagaaaaa
    22441 aatataatgt aaaagttggt aagtttaatt ttgctggaaa tggaagagct ttagcttctg
    22501 gtcaagaaca aggatatgtg aaggtagtag cagatgctaa atatggagaa attttaggta
    22561 tacatatgtt tggatgtggt gtagcagaac ttataaatca tgcagcatca tttaaagcat
    22621 tagagatacc tactgatgaa gcaagtgagt tgatatttgg tcatccatgt acttcagaag
    22681 ctttaatgga agctttggca gatgtaaatg gagaatgttt acacttacct aaaaaataaa
    22741 tctaaacata aattattaat ttaagtctat tattaatatt aatttatatc ataaaatttt
    22801 aaaacactta cttagccata ccccttatta tgcaaataat ttatttgcat aataaggggt
    22861 attttttatg gaaaaatata gaatatagaa tccttgtagt ttttgttgta caaaaaagat
    22921 agtaatataa aataacatta tcattttaga atgatttcaa ttgtaattag gaaaagtgat
    22981 ttttttgttt ttaaaaaaag tattatttgt aaaatataaa aaaaaatttc ttaatattta
    23041 aataaaagaa tagcttaatt taaatgttat tactttataa aaaaataata agtacaaaaa
    23101 aaacaatata tggcatataa gttgctatat aaataataag gagggaacaa ttataaatga
    23161 ggcaaagaat aaaaaactta attgaaaatg aagataagaa aaatccttta acagatgaag
    23221 cgatttcaag taggttaaat attcgaagag aagatgttac ctttttgaga aatgagttaa
    23281 aaatagaaga ttcaagacaa agaagaaaag ttgttttaat taaagctata aaagatattt
    23341 taaaagaaga aaaaactata aataaaaatg atataacaag aagattaaac agcattgggt
    23401 ttacagtatc aagatttaca gtcatacaat acttaaaaga gattcaagat tctggtgacc
    23461 taaaacttga taaagagagt gatgataaaa aagaagaaat aatcaataat agagaaaata
    23521 aagatatagc atttgataaa ctcataggta gtagtggtag cttaagtaca attataaaac
    23581 tagccaaagc agcaattctt tatccacctc atggtcttca tactattatt atggggccaa
    23641 ctggtgttgg aaagagtgag ttagcagagt gtatgtataa atttgcagta gaatcatcca
    23701 gattccctaa gaatgcacca tttatagttt ttaatgcagc agattattca gagaatccac
    23761 agcttttgct atcacagtta tttggacacg ttaaaggtgc ttttacagga gctgatgaga
    23821 acaaagaagg attagtatca aaggcagatg gaggaatatt gtttatagat gaaatacata
    23881 gattacctca tgaaggtcaa gaaatactat ttcaactgat tgataaaggt atggtcagaa
    23941 agttaggaga aacaagacta acacataaaa tagatgtaat gattatttca gcaacttctg
    24001 agccaataga ttctcattta cttaatacat ttaaaagacg tatacctgta acaattgaaa
    24061 taccagaatt agttgcaaga ccattaaatg agaggtttga cattataaat aatttcttct
    24121 taatagaagc tcaaagaatg ggagcaaaca tacatataaa gtcagatgta ttaaaagctc
    24181 ttatgcttta cgattgtata ggtaatgtag gtcaacttag aagtgatatt caagtagctt
    24241 gtgctagggg actattaaac caacttacaa acaaactaaa agaagtaaat ataagcatat
    24301 cagatgtacc tggatatgta aaacaaggtc ttataaaaat cagaaattgt agaggaaaaa
    24361 tagaaaatta tgtagatggg gatttaatta tagactcaaa tcttcaaggt gagatagaaa
    24421 agaaaaatga agatatatat acttttccag atgaaatata caaacttact gaaagaagac
    24481 atattgattt attggaacaa ggtttagatt ttgatgttat aaatagaatc attggaggag
    24541 aattagaaag ttgcattcaa aaatacataa aacaagttaa aaaagtttct attagtaaca
    24601 atcttccaga tataagtcca attgttggaa aatatatcgt agagctaact tatgaagtaa
    24661 tcaaaatagc atcacaatac ctaagttcac tagaaccgaa tctacattta tgtttagcag
    24721 ttcacctaaa agcagctttt gagagattaa aagaaggaaa agtaatatca aatgctcatt
    24781 tgaaagaaat aataggaaat tatccactag aatattctgt agcagaaaag gtagctcaat
    24841 atttaaagga aagatataac ataaacttac caaaagaaga aataggtata atagcaatat
    24901 attttaaaat gagtagtaga agagatatgt ctgaacaaaa aaagataggt gtacttctta
    24961 tggctcacgg aagagtagca tcttctgtat gtgaggttac aaataaacta ttaggaatta
    25021 atcacgcaag gtatttagat atgccatttg agaagaaacc ggaagaaatg attgaagaag
    25081 ctactacaat aataaaagat attgatgaag gtagaggtgt gattatatta gtagatatgg
    25141 gttcactttc atattttgga gatattattt ctggaaagac tggaattaat ataaggacaa
    25201 tatctagatt tgatacattg ctttctatag aggttatacg aagagcagtt ttaccagatt
    25261 taacaataga cgagatagtt gatgaagtta aaggtataga gccacaagta aaagaaaagg
    25321 ttagtaaatg taataataca aaagtagatt ttagaaaacc tataatttta acattatgta
    25381 ttactggatt agggggagct tgtaagataa aaaaaatcct agaggaaaat attaaaagta
    25441 taactgaaaa ggttgatata gaacctatag gtatgattga atatccaagt cttgatttag
    25501 agataaaaca aataagcaat gataaaaatg taatcgccat agttggaaca attgaccctc
    25561 agatagcaaa tatccctttt tttagtctag tagacataaa tgataaagca aagttaaatc
    25621 aaataaataa cctaattgat acaaatcaaa ttggtgaaac aaataaaaaa tatgaattat
    25681 cagatttaat acataaagaa gatatatttg tggatttaca ggttaaatca aaagaagaag
    25741 ttctaaaata tatgagtaaa aaacttagag agaaaggtta tgtgacaaaa aatttctata
    25801 aaaaagtagt ggaaagagat aacttatttc caactgaatt atcagatgaa atagcaatac
    25861 ctcacacaga tggaatagat gtaattaggc cagctataag tatagccatc ttaaagaagc
    25921 ctattgtgtg gagtaaaagt aaagtaaaaa taatactttt attagctgta gaccaaaagt
    25981 gttttaatcc attaagcact ctactctcat ttatagaaac agatgaattt aagaagataa
    26041 aaaacattaa agatagagat tttattaggg agatgatatt agatggagtc aatgaaaata
    26101 attagtagca atttaatttt taaaaatatt gaagtgtcta acaatgaaga tgcattaaag
    26161 tttttgggac aacgtttgtt tgacgaacag tatgtaaaag aaagctacat acaagctgtt
    26221 gtagcaagag aaaaaaagta tgctacaggg ttaccaacag aaatatacgg agtggcaata
    26281 ccacatacag atatagtaca tgtaaatgag ccaggaatag caattggaat actgaataaa
    26341 cctgtaaaat ttataatgat gggtacagac gatactgaaa ttgatgtaaa agttgtattt
    26401 atgttagcag tgaaagagcc acaagaacaa ttgcaactat tagaaaaact tatgactata
    26461 tttcaagata agaatatact aaataatatt gttgacttaa gtgaagaaag tgtgagtgat
    26521 ttattaaact ctaaattgaa aaattaaaca tgtaaagtat attaaaaatg taaaattaaa
    26581 catgcaaagt atgttaaaaa atataaaaag attattaaag atttctaact atagatatct
    26641 ttaaaataaa ataatttcaa tgggggtgac taatgtggct aaaaagatat tagttgcatg
    26701 tggaacagga gtatgtactt caacaattgc tataaacaaa ttaaagaaag cacttcaaga
    26761 cattggtaaa ttggatatgg ttaatattac acaatgtaaa gtagtagaag ttgcatcaaa
    26821 agcacctgat tatgatttaa taatttgtac tacacaagta tctagtagta taaaaacacc
    26881 agtgataaat ggattacctt ttttaactgg tgtcggaatg gataaattaa tcaatgatgt
    26941 attagaagag ttagaattat aattaaaaaa attaaggaac atggtctcta aaatcataag
    27001 ttttagagac ttaaaattaa ataatggagg gtctttatat ggagatatta aatttcattg
    27061 ttggtctagg tgcacaagta atgatgccaa taataatatg tatttttggt ttgatattag
    27121 gtacaaaact aggaaaatct ttaagagctg gtttaactgt gggagttggg tttataggtc
    27181 ttaacactat tatagctttg ctaacagata atttaggtcc agctacacaa caaatggtta
    27241 agaatttagg gctaagttta tcaataatag atgtaggatg gccagcagca tcagctatag
    27301 cttttgcatc aacagtaggg gctttaatta ttccaatagg tcttgtggta aatatagtta
    27361 tgctaattac caatactact caaactgttg atgtagacat atgggattac tggcattttg
    27421 cttttacagg agctttagtg gcaggggcta ctcaaagtgt aatgtgggga gtatttgcag
    27481 cagtagcaaa tatggtaatt gtattggtaa tggcagattt aactgctcca ggtatagagg
    27541 aatatttagg tatgccaggt atatctcttc ctcatggatt tacacaggca tttgttccaa
    27601 ttgctatagt agtaaataaa ttattagact taataccagg cataaataag attgagataa
    27661 atgctgatac attgcagaaa aaatttggat tatttggaga acctttaata atggggtctg
    27721 taataggtgt aataattggt attgctgcga aatatgatat aaaaggaata ttacaattag
    27781 gtgtaactat gggagctgta ttaatattaa ttccaaagat ggcagcatta ttaatggaag
    27841 gattattacc agtatcagaa gctgcacagg aatttattga gaagagattt aaaaacagag
    27901 gcaagattta tataggctta gattcagcag taggaatagg tcatcctgtt acattatcag
    27961 ttgcactagt gttggttcct ttgactatac ttatagcagc tatattgcca ggaaataaag
    28021 ttttaccatt tgcagattta gcagtaattc catttgcact tgtattaatc gtaccaatta
    28081 caaagggaaa tgtgtttaga acattaataa taggaataat tataattact tctggattat
    28141 taatcgcaac taacttagct ccattattta cccaaatggc tttaaatgct agttttaaaa
    28201 tgcctgaagg agcaactatg atttcaagta tttgtgatgg tgcgaatcca ttaagttggg
    28261 tatttgtaaa agttatgaac tataaagtta ttggaggagt agtttttgga gttatagctt
    28321 taggaatggc tatttacaat aggaatagaa taataaatga aaataaaaag ctatctatag
    28381 aagaataagg ttaaaacaat tttattaagg agtgtatagg ttatgaaaaa atataaatat
    28441 gattctaaaa gatacttact cagtgttaca ataccaggat tttttatgat tttaatattg
    28501 atttatgcac tgtttaataa ctatacaaat tttaatttga atatatatag tttgttaata
    28561 attgtctgta cttatggtat attcaatact tttatatcat caagtaatcc taaaaaaatt
    28621 attattgata gcaaatgtat acattttacc tcatttatga ctacacataa gtatgaaatt
    28681 aaagatatag aaaagataag aataagagaa ttttataata aaaaaattta tctaaaaatt
    28741 aatgatggaa aacttttcaa aggtaggtat tggataagaa caaatatgtt taataatagt
    28801 atagcattat atagctattt cattgagttg gaagagtgct tatatccaga ttcactaaaa
    28861 tttagaaata aaaaatttga gaataagaac atataaaaat aaaaaattga attgaagcct
    28921 agcttaatct tttttgataa gatataagtt tgaactttat actaaatttt tagaattttc
    28981 aatcaagaca ataacataat agctaagcaa ttagaacaca actaagatgt ataaggcatg
    29041 tatatgatga atgggaggat ttaattaatg ttattagaga aattaagaaa agaagtatta
    29101 caagcatctt tagacctttt aaactataat ttagtgacat taacaggagg aaatgtaagt
    29161 gggagagatg aacaaactgg ctatatagca atcaccccaa gtggaatgga ttatagaaat
    29221 cttacaccaa gtgatattgt tatagtagat gtagatggaa atattataga tggaaaatgg
    29281 aaagcatcag tggatttatc agaccacttg tatatatata agcatagaga agacataaat
    29341 agtataattc atacacattc tacatactca agttgttttg ctattttaaa tgaacctata
    29401 gaatgtgcaa gtacaactct tgcaaatgaa gtagggggtt cagtaccagt tgcaaaattt
    29461 tcgccaccaa catctaaaaa gatgggaaaa tgtgttattg aagcaattgg tgacaaaaga
    29521 gcatgtcttt tagcaaatca tggcgtaatt gcagttggtc catctgtagg acatgctctt
    29581 acagccgctg taatgttaga agattcagca aaagtatatt acttagcaaa atcaattggc
    29641 acaccagtgc tactgcctga tgaagaaatt caaagagcga gagatgtatt ttttaatgtc
    29701 tatggtcagg ataaataaat tagataaaat tttaaaagta atatgaaaat atagatattc
    29761 aatgatatta aggagattta taatgatata ataagtttaa tagttaagaa gtaaaatatg
    29821 aaatgtaata gtatataaat tcatttaaga ctaaaaagaa taagataaaa taaaaaggag
    29881 tctgtatttc tatgtattac ttattaaaca aaagcacaaa tccttacttt aatttggcat
    29941 tagaagaata tctattttta aatgataaat ataatgatga tattataatc atatggagaa
    30001 atgaagaatc catttttata ggtaaaaatc aaaatccata tcaggaagta tatcatgatg
    30061 ttattgaaaa gggagaaatt cctattttaa gaagaatatc tggaggagga actgtttatc
    30121 atgacttagg taatatcaat atgtcattca tacaaaaaga taggcaactt catgagatag
    30181 attttttgga gcatactaag tttatgcaaa agatgctttc aaccttagga ctagatgtat
    30241 ctataactga aagaaaagat ttatttttaa atgggaaaaa aatttcaggc tctgcacagt
    30301 ctataaaaag aaaaaattct ctttatcatg gaactcttct ttacgattca gatttaaata
    30361 aattaactaa atatttaaat tcaaataaag caactgaatc aaatgcaact aaatctgttt
    30421 caagtaaagt tacaaatatt aagtttttac tcgaaaaaga tataaattac tttttagatt
    30481 attgtgttga atatttaaaa tctaatataa agaatataaa agaacttgaa ttggatgaag
    30541 aagatattaa aagtgtatac aatataggag aagataaata taaaaagata gagtggacat
    30601 atggtaaaac gccgaaattt caagtagtgc taccatttaa taatgaaagt aaaataaata
    30661 tacatgttaa cagatggaaa gtatcaaaat tttttatttc ttatgatgat aatgtaatta
    30721 atatggacaa gttgttagat ttgaatttct ttaaagaaga aataaaagag agtattttag
    30781 aaaattatcc acattacata gatttaatca atttaatatt ttaatataaa acctctaatt
    30841 tgataaaatt gtcttatatc aaactagagg tttttaatat tcttgcatat taacaaccat
    30901 tacaaaattg taaaattaaa aacaacacta ttgaaaaatc aatatatatt gtaataaata
    30961 tatgtgtttt tcattagcaa atagacatta actatttata caactgaaaa tttaatatat
    31021 aattaatcag atggttaaaa taaaaatatt aaaagtttta ataaatgatg gttaaaaata
    31081 aaatacgagg tgataccatg tatagtgtta taattgttgc agcgggaagc ggaaggagaa
    31141 tgaacttaga tataaacaaa cagttcataa aattaagaga aaaagaaatt attgctcata
    31201 caattcaagt tttttatgaa aatataaata tagatgaaat agtggtatgt ataaaaaaag
    31261 aagaagaaga tttttttaaa gaaaatataa taaataagta taactttaaa aatataaaaa
    31321 tagcatatgg cggaaaagaa agacaggact caatatataa tggtttaaaa aaattagata
    31381 aaaattgtga tatagtactt atccatgatg gagcaagacc atttgtcgac catagaataa
    31441 tcaatgaatc tataaaggta gctaaagaaa aaaaggctgt ggttgtaggt gttccagtaa
    31501 gtgatactat aaagatagtg tcagatggaa cagtacaaga aactccagaa agaaatttgc
    31561 tgtgggcagc acaaacccct caaacctttg aatataatct cataatagat gcttatgaac
    31621 aagcatataa gaacaattat tatggtactg atgattcaat gttggtagaa aatataggtc
    31681 aaagtgtcac tatggtaatg ggttcttatg aaaacataaa aataactagt ccagaagatt
    31741 taaatatagc agaacaaata ttaaatatgg aaaaaagaga tgaagttaac agtagaagga
    31801 gaattatatg agaataggat taggatatga tgtacacaaa ttaacagagg atagaaaact
    31861 tataatcggt ggtgtagaga taccacacga taaaggtttg ttgggtcatt ctgatgctga
    31921 tgtattgata catgcaataa tggattctat tttaggtgct ctagcattag gagatatagg
    31981 gaagcatttc ccagatacag atgaagaata taagggagca gatagtatga aattattaga
    32041 acatgtgtat aatctaataa caagtaaagg atataaaatt ggtaatatag acagtactat
    32101 aattgctcaa agccctaaga tggctccata tattgagagt atgagaagta atatatcaaa
    32161 agttttaaac acagacattg ataatataaa cataaaggca acaacagaag aaggtcttgg
    32221 ttttacagga gcaaaacagg gaatagcttc acaaagtatt tgtttattat tgttgacaag
    32281 tcagaataat taatatatta ttagtaataa gtttaatgga tatcaagtaa taggcctata
    32341 tagaatatct ataatgtatg aatttaagtg aaattaatat aaatataaaa ttagctatga
    32401 taaagaaata gtaaaatgat gaaatcttca cagagagaaa acggtggtga gagttttcaa
    32461 gaggaagtct ttgaacctgt cttttagctt aaaggtataa aaatctttac ggtaggaacc
    32521 gttatattcc caaaagagag ctattcatat agctaactag agtggtaccg cggaaattaa
    32581 acctttcgtc tctatacttg tatagagatg agaggttttt tatattttca ggaagttaat
    32641 attcaggagg aatctattaa tgaaaatgtc aaaaatgttt atgccgacat taaaagaaat
    32701 cccagcagat gctgagatta caagccatca attaatggtt aggtcaggca tgataaaaaa
    32761 aatgacttca ggtgtttata accaattacc aatggggtta agagtgttca aaaaaattga
    32821 acaaataatt agagaagaat taaataaaaa agattgtcaa gaaatacttt gtgcagcctt
    32881 acttccttct gaattatgga aagagtctgg aaggtggact gctatgggtg aagaaatgtt
    32941 tagactaaaa gacagaacag aaagagagta ctgtcttggg ccaacacatg aagaagcatt
    33001 cactgatata ataagacaag aaattacttc atataaacaa ttgccactga acttgtacca
    33061 aatacaagta aaatatagag acgagagaag accaagattt ggtgttatga gaactaagac
    33121 ttttacaatg aaggatgcct atagttttga tgtagatgat aaaggtttag ataagtcata
    33181 tcaagatatg tttgatgcat atgtaagtat atttaataga tgtggattgg agaatagccc
    33241 tgtacaggca gattcaggag ctataggagg ttctacatca gccgaattta tggttaagtc
    33301 agaagttggt gaagatgaag ttgtattttg tagtggatgt gattatgcag ccaatgtaga
    33361 aagagcagaa tcttgtaatc tagcatcaca aaaagaagaa atgaaagaat tagaagaggt
    33421 tcatacacca ggagcagcta caataaaaga gttagaagaa tttttaaaaa cttctccaga
    33481 taagtttgct aaaactttag tatatgaggc agatggaaaa acagttgtag tcgttgtaag
    33541 aggagataga gaagttaatg aaatcaaagt atctaatgcc ataggttctg ttatagaatt
    33601 tgctcttgct acagatgatg tagtgagaaa agtaactaat gcagaagtag gatttgcagg
    33661 tccaataggt ataaatgctg actatgtatt tatagataaa gaaattgttg aacaaagaaa
    33721 catagttgtt ggggctaata aaactgagta ccatataaaa aatgctaact atggaagaga
    33781 ttttgaagga atagttggag actttagaaa tgttcaagaa ggcgacaagt gtatagtatg
    33841 tggtaagcca cttgaaatag ctagaggtgt tgaagtagga catatattta aactaggaac
    33901 taagtactca gaatctatga atgcaaactt catagacaaa gatggaaaat caaagcctat
    33961 agtaatggga tgttatggaa taggtgttga aagaacagca gcagctataa ttgaacaaca
    34021 taatgatgag aaaggtataa tatggccttt gtcagtagct ccataccatg ttgtgatagt
    34081 tccagcaaat atgaaaaatg aagaacaaat atcaatagct gaaaatatat ataatgattt
    34141 gcaagctatg ggtgtagaag ttttattaga tgacagagat gaaagaatag gtgtcaaatt
    34201 caatgattca gaattaatag gaatacctat gaggattaca gtaggtaaaa atattaatga
    34261 aggtaaagta gaatttaaac ttagacataa agaagataaa gagataattg acatagaaga
    34321 aataaatgaa aaagtaaagg cagagtttat caggaataat gttaggttag gataataact
    34381 taaataagat attgtataga taatcaaagt tgtactttat agacaccatt ttatctatat
    34441 ataaggatat aaaatggtgt ttttttataa aacaaataaa tgaggaggta attaaaatgg
    34501 caaaaaatga aaaacaattt gttgaagaaa taacaaagat ggaagatgat tttccacaat
    34561 ggtacacaga tgtaataact aagactgact tagttgatta tgccccagta aaaggtttta
    34621 tggttgtaaa gccatatgga tatgcattat gggaaaagat gcaagaattt atggataaaa
    34681 aatttaaaga aactggacat aaaaactgtt attttccatt attaatacca gagagtttat
    34741 taaacaaaga agcagaacac gttgaaggtt ttgctccaga agttgcatgg gtaacacatg
    34801 gaggaaataa aaaattagaa gaaagattat gtgtaagacc tacttcagag actataatat
    34861 gtacaatgta tgcgaaatgg ttaaaatcat atagagaatt accatatctt tataatcaat
    34921 ggtgctcagt tgttagatgg gaaaaatcaa ctagaccttt cttaagaaca tctgagtttt
    34981 tatggcaaga aggtcatacg ttacatgaaa cagcagaaga agcacaagaa gaaacaatac
    35041 aacaattaga agtatataaa gcgctatgtg aagaattact tgcaatgcca gttgtagctg
    35101 gtcaaaaaag tgaaagtgag aaatttgcag gtggtgaaag aacatatact atagaagcta
    35161 tgatgcatga tggtaaagca ttacaatcag gtacaagtca cttcctaggt caacacttta
    35221 ctaaggcttt tgatattact tttgcagata gggaaggaaa tttagctaat ccataccata
    35281 catcttgggg agcatctaca agacttatag gtggacttat aatgactcat agtgataaca
    35341 gaggtttagt attaccacca agagtagctc caattcaagt tgttatagtt cctatagctg
    35401 ctaaaaaagg aaatgtaatg gagactgttg ataaaatata tgctgattta aaagctaagg
    35461 gagtagctgt agaagttgat gatagagata actatacaac tggatggaaa tttaatgagt
    35521 gggaaatgaa aggtgttcca gtaagagttg aaattggacc aaaagatata gaaaataatc
    35581 aagctatggt atttagaaga gatactttag aaaaggattc tatgccttta gaaggattag
    35641 ctgatgctat atgtgattta tttgatgtta tacacaatga tatgtttgaa aaagcaagaa
    35701 aacatagaga agacaataca tctatagttg aaaatatgga tgagtttaga aaagctttag
    35761 aagaaaagcc aggattcata aagactatgt ggtgtggaga tgcagaatgt gaagctaaga
    35821 ttaaggaaga aacaggagca actataagat gcttaccttt tgaacaagaa aacttaggtc
    35881 acaagtgtgt gtactgtgga aaagaagcag atagtatggt agtaatggca aaagcttact
    35941 aaaatacatt taaataaaaa ctggggtaga aagtatttaa tactttatcc cagttttttg
    36001 ttttttatga gtaataaaat gtatttgtaa ctatgtcata agataagtca tagatactta
    36061 tattaaaaag aatataatag agaactattt ttaataatta catattaatg aaagcaaaat
    36121 attttgttat atagattttc aataaagcaa acaataaata taaatgaata ttaataagga
    36181 gtgcttttaa tttttagaaa ataaatgact taaaagaaag atttatgaaa ttaaaactag
    36241 gattttctga tataataaac atgtgtattt ttaaataaaa aggatattat tattagattt
    36301 gattaaaaat aaacttgcaa aacaatgtac tagtttaata ttatagttat attgaataaa
    36361 atatcaggag gaagtaaaaa tgaatgtaag agtaaggttt gcaccaagtc caacaggatt
    36421 tgtacatata ggtagtttga gaactgcttt atataactat ttatttgcaa aaaaaatggg
    36481 cggagaatat atattaagag tggaagatac agaccaaagt agattagtag aaggtgctat
    36541 agagaatatg cttaatgcta tgaaatgggc tggggtcaac catagtgaag gtgttatatt
    36601 agatgatagt ggaaaagtag tacaaaaagg agaatatggt ccatatattc aatctcaaag
    36661 attagatata taccaagaat atataaagca acttttagat agtggaaaag cttattactg
    36721 tttctgtaca aaagaaagac tggatgaagt aagagatgct caaagagcag caggagaaac
    36781 agctaaatat gatggtcatt gtaagaatct ttctaaagaa gaagtagaag cgaatataaa
    36841 agcaggaata ccatatgtta taagattaag attgccagag aatcatacaa ttaagtttac
    36901 agacttagtt agaggagata tggaatttaa tactaatgac ttagatgacc aagtattaat
    36961 gaaaacagat ggatttccaa cttatcattt tgcagtagtt gttgatgact atttgatgaa
    37021 aattacacat gttataagag gagaagaatg ggtatcatct actccaaaac atgtttattt
    37081 atatgaagct tttggatggg aagcacctgt atttgtgcat ttaccaaata tacttaataa
    37141 agaaaagaaa aaattaagta aaagacaagg tgatgtagct gttgaagact ttaagaaaaa
    37201 aggatattta ccagagggat tagtaaacta tgtagcatta gttggttggt ctcctgaaga
    37261 taatcaagaa ttgtttacaa tggaggaatt agaaaaagcc ttctctgtag aaagagtatc
    37321 taaaagtggt ggagtatttg atactgaaaa attaaattgg gttaatcagc actatataaa
    37381 agatggtgat gatgcttatc taacagactt agcaatacca tttttaatag aagatggatt
    37441 tataacagaa gaagaagcta ctaataaata tgatttctta aagagtatga tatcagtact
    37501 taaagagaaa ttacaatatg taaaagaagt tactgaacat gcaagtatat tttttggaga
    37561 taaaatagag gtagaaactg aagagggaag agagttctta agattagaac atatacctac
    37621 tttaatagat gctttgagag aaaaaataga aaaaacagag gtattaaatg ctgagtttgt
    37681 tcaagctatg cttaaagaaa tacaaaaaga gtacaaaata aaagggaaaa atttatttat
    37741 gggctcaaga ataattttga cagggcaaat gcatggtcca gatttaccaa aagtaatgga
    37801 agttttagga aaagaaactt gcttaaatag aatagcttat gtaaaaaata atatattata
    37861 gtcataaaag tccgctaatt tagaagcgga ctttttttgt gttagtattt ggtgaaaata
    37921 tctgcttaag aaaatgaagg tatttaaggt aataaattta atatataaaa agcaaagcaa
    37981 aaatataaat aaaatatgaa taataaacta ataaaatatt aagcatagaa gaagggaatc
    38041 tattatttta gtttgattta attattaagg gggtaattac tatgtatagt aatattgatg
    38101 atgttaaaaa ggaattaaag gagctatgct tagaatatgt tacaatttta gaaaaattaa
    38161 aagatgaaaa aatgattaca gaagaaactt ttgaaaagtg ctcttctcaa aaaaaaatat
    38221 ttttagaaga acaatagagg atggtaatag tattattatt atgtagtttg aaagatagta
    38281 cgaaaaatga tagtaatact attatttatt gcaattttca actataatat aatatataat
    38341 gttaagaaga taatttataa aaatacagtg aggtgatgag tgtgaaagtt tataatacat
    38401 tgactagaac aaaagaagaa tttgttccat tagaagaagg aaaggtaaaa atgtatgtgt
    38461 gtggtccaac agtttataac tatatacaca taggtaatgc aagaccattt ataatatttg
    38521 atactctaag aaggtattta gagtatagag gatatgatgt aacttatgta caaaacttta
    38581 cagatgttga tgataagatt ataaatagaa gtcatgagga gggaatatct cctgaagaag
    38641 ttgcggctaa gtatataaaa gaatattttg tagactgtga tgggctagga ataaaaagag
    38701 ctactgttca tcctcaggtt acagataata tacaacagat tatagagttt ataaaagagt
    38761 tggaagataa gggatatgca tatgcagtaa atggtgatgt atattttgat acaaataagt
    38821 ttgaaggtta tggaaaactt tcaggtcaaa aacaagaaga tttagaggca ggggctagaa
    38881 ttgaggttaa tgaccaaaaa agacatccta tggactttgt tctttggaag gcaaaaaaag
    38941 aaggtgagcc aggatgggat agcccttggg gagaaggtag accaggatgg catatagaat
    39001 gttctgttat gtctaagaga tatttaggtg aaacaattga tatacatgca ggtgggcaag
    39061 atttaacatt cccacatcat gaaaatgaaa tagctcaaag tgaagctaga agtggaaaaa
    39121 cattctcaaa atactggatg cataatggat atataaatat aaatgatgaa aagatgagta
    39181 aatcaaaagg taatttcttt acagtaagag atatatcaaa attatatgat ttagagatag
    39241 taagattttt catgttatca gcacattata gaaatccagt aaactttagt gatgagatgt
    39301 taaatcaagc taaagcaggt cttgaaagac tatataatac taaagaaaaa ttagaattta
    39361 cgctaagtaa tttagtagaa tctcctttaa cagaaaaaga agtagagctt gttaaagaat
    39421 tagatgactt tagacaaaaa ttcatagatg ctatggatga tgatgttaat acagcagacg
    39481 cagtaagtgt aatatttgaa ttagctaagt taataaattc taatgtagat gaaaattctt
    39541 ctttggagtt tgctaaaaaa tgcttagatg aatttaatga acttacagga gtattaaata
    39601 ttgtaaataa gaaaaaggat actgtattag ataaagacat tgaagaattg atacaaaaaa
    39661 gaactgatgc aaagaaaaat aaagagtttc aattagctga tgatattaga caacaattac
    39721 tagatatggg aattgtatta gaagacacaa gacaaggtgt taagtggaaa aggatataaa
    39781 aatggaaaaa actgaattag ttactatgtc accattagtt ttagcatact taggtgatac
    39841 agtgtatgag acatatataa gagaatattt aattagacag aatacacaaa gaaaagttaa
    39901 tgatttgcat aaactagcta taaagtatgt aaaagctaaa gctcaggcaa ctataattca
    39961 tgaagttgaa attgagttaa ctgaagaaga gagtaaaatt tataaaaggg gaagaaatca
    40021 aaagtcaaat acttctccta agaatgcaga tattatagac tacaaacatg caactgggtt
    40081 tgaggcatta gttggatatt tgtatttaaa taatgaaata gagagacttc aatatattat
    40141 caataaagga atcaaaatta ttgaaagaga tatgtaaata ttaaagagtg ttgtcttaat
    40201 aataagtaat aaggcaacac tttttttata actaatgtat aataaatata tagaaattag
    40261 taatgagaaa ggataaaaag ttatgaaagt aaaattaata tcacatacac ctgaaccaga
    40321 aaaagtaata gctatggcag ctaagctatg ttattcacca gttgggacag acgaaataga
    40381 aaaagactta acagatgaaa gtatagagaa atttttaaat atgttattaa gtataggtca
    40441 tgggtcaata ttagaacatg cttcatttac attttcaata gaaggtattt caagggcatg
    40501 ttcacatcaa attgttagac atcgtatagc tagtttttca caacaaagtc aaagatatgt
    40561 taaattagaa caatttgagt acataattcc tccagaaata gaaaaaatag aaaaggcaaa
    40621 agagttattt atagactcca tgaaaaaaga ccaagagaat tatgataagt tagttgaaat
    40681 attgtttgaa aatcattata atgacctaat aaaaaacggt aaaaatgaga aaacagctaa
    40741 aagacaagct gagaagaaag caatagaaga tgctagatac gtatttccaa atgcatgtga
    40801 aacaaagatg gtatttacta taaatgctag aagtttgttt aatttctttg aacatagatg
    40861 ctgtgaaaga gcacagtggg aaataagaaa tttagctgtg gagatgttaa gagaggttaa
    40921 aaaggttgct cctatattat ttaaaaagac tggtccaagt tgtgtaaatg gaagttgtcc
    40981 agaaggaact atgacttgtg gagatatagt tcaagttaga gaaaaattta aggccttgta
    41041 ggaggtgaat aatttggcga gtatagaagg aagaaatccg gtaatagaag caataaaaag
    41101 cgacagagaa atagataaaa tattaattgc aaattcagct aaagaaggtt caattaagaa
    41161 aataatagga atggctaaag ataagaatat aattattcag tatgttgata aacataagtt
    41221 agatgaagta agtacaagtc attcacatca aggtgtaata gcttatgcaa gtgagtataa
    41281 gtattatgaa ttagatgagc taatagattt agcaaagaat aaagatgaag acccattttt
    41341 cattatatta gatgagataa cagacccaca taatttgggt tcaataataa ggacagcaga
    41401 tgcagtagga gcacatggag ttatcattcc aaaaagaagg tctgtacaca taactcctgt
    41461 agttgccaaa gcatctgctg gagcagtaga atatatgcca gtttgcaagg taaccaatat
    41521 agttaataca ataaaaagac tcaaagaaga agggctgtgg attgctgcag ctgatatgga
    41581 tggagaaact ttttataaac aaaatcttac tggtcctctt ggtgtagtaa ttggaagtga
    41641 aggttttgga atatctagat tggttaaaca aaactgtgac tttatagtaa aaatgccaat
    41701 gataggtaat gttacatctt taaatgcatc tgtagcaggt ggaatacttt tatatgagat
    41761 attcagacaa agactggata aaagtaaata ggcatgaaaa aaaatataaa tcattactta
    41821 ataatagatg gctacaatat tataaatgcg tgggacaatt tgaaagaact ggctaaagag
    41881 gacttagaag attctcgaga aaagcttatt gatgacatta tagaattttc tgagtttatg
    41941 ggttataaga ctataatagt ttttgatgct tataatgtta aaaattctcg agaaaaagtt
    42001 gaaaaaagaa aacacataac aatagtttat acaagagaac accaaacagc agatagttat
    42061 atagagaaat ttataacttc tttatcaaag tatgatgatg taaaagtagc cactaatgac
    42121 tatgcagaac agcaaatgat tttaggaaaa ggtgctacta gaatgtctgc aagagaatta
    42181 aagttagagt tagatcgttc caagaataaa atgaaggaaa aaaatatcgg tttgaggaaa
    42241 aaaattcagc gaaattggtt ggaggaaagg ttagataaag aaacattgtc gaaacttgag
    42301 aacattcgta gaaagcgttg aaattactag gttctttaga ctataatata cctataaatt
    42361 tgttttatat atgggggaga tttacatgtt ggtagcaaaa gaaaaaagtt atgagttagt
    42421 ggacaattgt cagcaggatg agtataacat tgtattaaga gcaagtgaag gggataaaat
    42481 agcactagag tacattatta ctaaatacag aaattttgta aaggcaaaag caaagtcata
    42541 ttttttaatt ggagcagata aagaagatat aatacaagaa ggaatgatag gcctttataa
    42601 ggcagttaga gattttgatg gaagtaagac caattcattt aaatgttttg cggagatatg
    42661 tataacaaga caaataataa ctgctataaa aactgcaaca agacaaaaac atataccttt
    42721 aaattcatat gtttctttga ataaaccaat atatgatgaa gagtcagaca gaacactttt
    42781 agatattata gctacaagta tagtaacaga tccagaagag cttataatta gtaaagaaga
    42841 gttaaaaaat atagaatcaa aaatgaacga attgttaagt gacttggaat tagaagtact
    42901 agaattatat ttaaatggaa agtcatacca gtttatagcg gacaagctta aaagagatgt
    42961 aaagtcaata gataacgctt tacaaagggt taagagaaaa ttagaaaaac atcttgaaaa
    43021 cagaaatgat taatagtaaa atacttacca taagcttatt gtgagcaaaa ttaatttata
    43081 tcgaaagata agggaggaaa ttcaaaatgg ctaaagctaa atacgaaaga acaaaacctc
    43141 atgttaatat agggacaata ggacacgtag accacggtaa aactacatta acagcagcaa
    43201 taacaaaaac attatatgac agatatcaat taggagaagc agtagatttc gcaaacatag
    43261 ataaagctcc agaagaaaga gaaagaggaa tcacaatatc aacagcacac gttgagtatg
    43321 aaacaccaaa tagacactat gcacacgttg actgcccagg acatgctgac tacgttaaga
    43381 acatgataac aggagcagca caaatggacg gagcaatatt agtttgttca gcaacagatg
    43441 gaccaatgcc acaaacaaga gagcatatac tattatcaag acaagttgga gtaccatata
    43501 tagtagtatt cttaaacaaa tgtgacatgg tagatgatga agagttatta gagttagtag
    43561 agatggaagt aagagattta ttaacagaat atgatttccc aggagatgac actccaatag
    43621 taagaggttc agcattaat
//
LOCUS       DAMJYR010000025        40856 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.025, whole genome shotgun sequence.
ACCESSION   DAMJYR010000025 DAMJYR010000000
VERSION     DAMJYR010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 40856)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40856)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40856
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.025"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(130..837)
                     /locus_tag="PC471_001262"
     CDS             complement(130..837)
                     /locus_tag="PC471_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HDF2788400.1"
                     /translation="MMNIKNKKHILKKFIAMVLIAGVVTVEAGAITASAAEPTNSPMS
                     ATVDQCDFLNVRSGASANDAVVGKINTGDKVEVLELHSNGWIKIKSVDNVTGWVNGDY
                     LTIQGGNVDAKVQNVLNLAFKQQGKPYKWGATGPNSFDCSGFTSYVYKNGAGVNLPRV
                     SRSQATVGKKVSRAELKSGDLVFFGSGGSINHVGLYVGDSKFIHSPQTGDVVKVTSMA
                     PGTNYARRLITATRVLQ"
     gene            complement(1036..2949)
                     /locus_tag="PC471_001263"
     CDS             complement(1036..2949)
                     /locus_tag="PC471_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HDF2788401.1"
                     /translation="MRESIDLEKESFFNRLFKKYKFRHLTLLILDICSVLVAFRMSLS
                     LTGNLNAFTTNDIIISACIYIVLHIVSFRLFKCYNTLWRYAGEEEIISIFVATLAYLI
                     PIYIINKLLGFDYPIMFYVLNTIFIIMFTSGARIAYRAIRIVMNKTYSRGKVSNILII
                     GAGDAGEMVIQELKRNPELKKVAVAIIDDDKNKIGRIIHNVKIVGTTSDIKDVVEKYN
                     VDEIIFSIANIEKRRKKEIIDICKNTNCKIKTIPGIYEIIDGKVDIKQIREVEIEDLL
                     GREPIKTNLREISNYIEGKVILITGGGGSIGSELCRQIAGFNPKELIIVDNYENNAYS
                     IQQELLRKYKNKLDLKTVIASIREEKRMDEIFNKYKPEVVFHAAAHKHVPLMESSPGE
                     AIKNNIFGTLNIAGLSSKYRAKKFVLISTDKAVNPTNIMGATKRAAEMIIQTMNAESQ
                     TEFVAVRFGNVLGSNGSVIPLFKKQIEDGGPVTVTHPDIIRYFMTIPEAVGLVIQAGA
                     MAKGGEIFVLDMGEPVRILDLAKNLIKFSGFEPDVDIKIEFSGLRPGEKLYEELLMSE
                     EGLLDTEHKKIFIGRPIDVDREKITKYLKLLREITNNEEVEKIDGIMRELVPTYIKPE
                     DANIKEIATTREK"
     gene            complement(3033..4124)
                     /locus_tag="PC471_001264"
     CDS             complement(3033..4124)
                     /locus_tag="PC471_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDF2788402.1"
                     /translation="MKTVCYLADGSNPHTLKWCSFFKNKGYDIHVISLNGGHMEGIKI
                     YNFGSNVEELKNENISKKTGYLGSIKQIKKLVNEIKPDILHAQYASSYGFIGSLLGYH
                     PYIISVWGTDIYDFPNNGFIQKQIIKHNFRKADYIFSNSRDMAKEANKYTAKHVDVTF
                     FGVDMDRFKPMEVEKEDAFVIGIIKSLEKKYGIEYLIQAFKMLKDEYKDKKIILKIGG
                     SGSQMDNLINLAKELGIENDVQFLGRISPENVSKTFNSFDVTVFPSLREGFGVAAIES
                     EACEVPVIVTNVGGHPESVWENETGLIVEPKQPEEIKNAIIKLMENDELRLNMGKKGR
                     QFVRENYEVNLNFNDIEKIYDSIFDKYKK"
     gene            complement(4130..5497)
                     /locus_tag="PC471_001265"
     CDS             complement(4130..5497)
                     /locus_tag="PC471_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HDF2788403.1"
                     /translation="MYFVSMFLMPLKKEYKFYILGGKKMKIAIAGTGYVGLVTGVCLA
                     EVGNENVVCVDVDEQKVKSMKLGIAPIYEEGLEELMVKNYNRGRIDYTTDYKKAYEDV
                     DVILIAVGTPERSDGSANLDYIKAVAKQIAETVTKDTLVVIKSTVPIGTNEEIEDYIN
                     RNLKNDVKVELASNPEFLSQGTAVKDTLHASRIVIGVESEWAREILEEIYKPFNQPII
                     VTNRNSAEMIKYASNNFLALKISFMNDIANLCEIAGANIEDVALGMSYDDRIGSKFLK
                     AGIGYGGSCFPKDTKALHWLSEHNGYELKTVKAAIEVNESQKLKLVRKASKLVESFQD
                     MKVAVLGLTFKPDTDDIREAPSIPNIKYLLERGARVTVYDPIGVNNTKKIFGDSILYA
                     DSVDDAIEDAEICFIMTEWKDVLNYDIHKYKEKMKNPLVLDGRNCYKIKDMEELGIEY
                     YSIGR"
     gene            complement(5451..6203)
                     /locus_tag="PC471_001266"
     CDS             complement(5451..6203)
                     /locus_tag="PC471_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HDF2788404.1"
                     /translation="MNEPLVSIITPVYNSEEFLSETIKSIQNQTYKNWQLLLVDDCSK
                     DNSSEIIKSFRKEDARIKYIKLEKNSGAAVSRNVGIKNAEGRFIAFVDSDDLWDSRKL
                     EIQIEYMLKENVGFSFTSYRYMRQDGSKTNKVARAPKKIDYEGLLRNTIIGCSTVVID
                     KEIVGEFSMPLVRRGQDTATWLQLLKKEKYAYGIQEDLVNYRLVGNSISSNKIKALKR
                     TWNTYRNVENLSLPKSLYVFCFYVFNAIKKRV"
     gene            complement(6251..7159)
                     /locus_tag="PC471_001267"
     CDS             complement(6251..7159)
                     /locus_tag="PC471_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HDF2788405.1"
                     /translation="MYKYKFTVFTPTYNRAHLLENLYNDLKAQTYDFNDFEWLIVDDG
                     SSDSTKELVEKFISENKLNIRYIYKENGGKHTAINLGVKNADGELFFIVDSDDGLIKD
                     SLEIANDEWDSLENKEGFSGVVGLCVYPSGDLIGTEMPEDKKICHFADLYFKYGVKGD
                     KSIVFVTDELIKFPFPERKEVRFLPESVVWNEMSKYYKVICVNKPMIIRDYLDDGLTK
                     NILSKNALRGRALEFLYLINQNTYPLSRYPYMWIKNYINLARYSLLSDSHYFGELRKV
                     SDKLLYLALFPLGYYKYIGQRKLVSK"
     gene            complement(7181..7930)
                     /locus_tag="PC471_001268"
     CDS             complement(7181..7930)
                     /locus_tag="PC471_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HDF2788406.1"
                     /translation="MKKNLVSIITPMYNSEKFIEATIKSVLNQTYQEWEMLIIDDCST
                     DNSPNIVKSYMQQDSRIKCIKTETNKGVSNARNLALSKATGQFIAFLDSDDQWNSSKL
                     EKQVNFMLENDYVISFTSYELMDENDKKLNKVIKVPPNVDYRRLLKGNILGCLTVVID
                     KSKLDFEIRMSGVRHEDYVLWLSILKKGHIAHGINEVLALYRKSSNSLSGNKIKAAMW
                     TWNIYRNIEKIPLYKAIYYFINYGINGIKKS"
     gene            complement(7959..9134)
                     /locus_tag="PC471_001269"
     CDS             complement(7959..9134)
                     /locus_tag="PC471_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glycerol glycerophosphotransferase family
                     protein"
                     /protein_id="HDF2788407.1"
                     /translation="MDKVKFLINMLLAFFIYPFNKHKFNGRDIWLIGGHSGDIYNDNS
                     KFFYEYMLKEHNDVETYWVVNKDSKVFDKIPGKKLIRGSVENYLYYYNSKAIVFSHAP
                     SADIAPYNFAVPVLNYFHKKTIKVFLNHGTISFKKRKPMNKKFKNIIDNLYKSYNIVT
                     ASSEFERNVMVNDWGMLDDSVYIIGNARYDNLPTNEVAQTRDILYTPTWRDWIKFSSG
                     KFTDTDYFKNIMNFLNDDKLNKILDEKDINVKIYMHHLMHEFIDDIKENITGKRIVFL
                     DKGVTLANEIRKSAANITDYSSVAIDFLYMNRPILFYQFDLDEYMEKVDSYIDLKSEM
                     FGSLAYNNDEAVNKLIDIIENNFEVMDNQKNERNKFFRYNDNKNCKRIYDCVLSKIK"
     gene            complement(9149..9988)
                     /locus_tag="PC471_001270"
     CDS             complement(9149..9988)
                     /locus_tag="PC471_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HDF2788408.1"
                     /translation="MLISLIMPTLNRYDDIYLLMDSLENQTYKNFELIVVDQNDNSKV
                     KEIVDKYIDKLDIKYIKSSKKGLSYNRNVGIDNAVGQIIGFPDDDCVYENDTLEKVIN
                     FFNKNKDYKIYSCKTMDSNKVDAFKKMYDGTCDITSSNVLDTITSITFFIDFEGKDYT
                     RFDEKLGVGGEFGAGEEIDYVLNLLSLGFKGKYFGNDIIYHPAKKHSKSKEKYQKDYN
                     YGRGFGALCKKEIVYRKNYKFAKVMVSKLVRNIGGLILSSNRDYHSATIKGRINGFRQ
                     YKL"
     gene            complement(9991..11148)
                     /locus_tag="PC471_001271"
     CDS             complement(9991..11148)
                     /locus_tag="PC471_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide polymerase"
                     /protein_id="HDF2788409.1"
                     /translation="MKTLNDDRNIMIRKYLYIFVLFIILLRKYMISFIDPNMDIGMIK
                     SALFYSSIGILMLLFLFDKRKSITEMVLVGVCVLLYLLNREGAILLIVLLAVSAKQID
                     DKFIVKNYLIISACFLMVAILLFNLFPSLIFNQEVHYRYIEKIDMLVTRMDFGLGNPN
                     SVFYHMVTIYAAYIFLRYKDYNKWDRIILFGSAFFVYQTTYSRTGFFTILAGLIFVEI
                     IRWIDIKKIKGLPMLLKTLPIILTLFSVIIGTVFDKSTLLNRLLASRPKFWHVYLAEK
                     GNFLKPFGNSYSPAIKATNPLDSSYVYIISVLGVVACILFMYLMYKGIESFIEKDKKA
                     YLVALFIFLLYSFAENLLLEASFSFAVVLLIKEVILNDKREIDLWKMKSRR"
     gene            complement(11164..11886)
                     /locus_tag="PC471_001272"
     CDS             complement(11164..11886)
                     /locus_tag="PC471_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDF2788410.1"
                     /translation="MIPKKIHYVWFGGPKGNIENICINSWKEKLPEYEIVEWNEKNFD
                     IEKEIKGNKFLEECYKRKLWAFISDYTRIKVLYEQGGVYMDTDMQILKDITPLLENNR
                     LICGYEDDREYINGAIIGVEKGHPFLKDLLEYYEKEVLTSSLFTIPKIMTHLMEKNYK
                     KIDPNNYEEGIRVYDKEYFYPFGFKEDFTPECITENTFGIHWWGKSWAKKRNYFLESK
                     HLTGVNKIWKCCKIFASNTLRS"
     gene            complement(11898..12971)
                     /locus_tag="PC471_001273"
     CDS             complement(11898..12971)
                     /locus_tag="PC471_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanylyltransferase"
                     /protein_id="HDF2788411.1"
                     /translation="MKVYNVIMAGGGGTRFWPLSRQEVPKQLINLSGEDALINETINR
                     IDSLAKKDDLFIVTNEKQLEALKDIVKDKCLDSNILPEPCARNTAAAIGFAAFNIMKK
                     YGDGVMCVYPADHYIKDEKEFKSILEKAIYIAENNDKLVTIGITPTFPSTGYGYINFN
                     RENTIEDVAYEVVEFVEKPNYEIAKEYVNSKKYVWNSGMFVWKVSKILEDFKRYLPKV
                     YEKLEDISKYLGTKEEMEKIKEIYPTIQSISIDYGIMERSNDVIVVPGDFGWNDVGSW
                     DSLGAIYPTDDEGNIKRGENITIDTKNSIIYSDDKLISTIGISDLIVVSTNDAVMVCR
                     KDKAQDVKKIVEQLKEEDRQEYM"
     gene            complement(12993..14699)
                     /locus_tag="PC471_001274"
     CDS             complement(12993..14699)
                     /locus_tag="PC471_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HDF2788412.1"
                     /translation="MDYKNNYEMWLNSPYFDEQTKNELLSIKDDEKEIQDRFYKNLEF
                     GTGGLRGIIGAGTNRINIYTVRRATLGVLNYIMKTQGEEGKQKGIVIAHDSRYMSREF
                     CIEVAKTLSAYGVKAYIFEELKPTPELSFAVRYLKCAMGIVITASHNPKEYNGYKVYD
                     SDGGQICIDMANDIIAEVNKIDDYSTIKSIDFEEALSKNLITILDNEVDDEFIKAVKK
                     QVLRQNIIDEYGKKLKIIYTPIHGTGNKPVRKVLNECGFENVMVVKEQELPDSNFSTV
                     KYPNPEEKSVFNIAIEMAKNNGTDLIIGTDPDCDRVGIVVKDSSGEYVVLNGNQVGSL
                     LVRYILESLVEENKLPKNNPTIIKTIVTSELGAKIAKAYNVDCLNTLTGFKFIGEKIK
                     AFEESNDRSFIMGYEESYGYLIGTHARDKDGVVSSLMICEMAAYYSSKGMNLYEALID
                     TYNKFGYYKEDLKSVTLKGIDGIKKIKEMMLYFRSVKIDNVADVKVDKILDYKDGVDD
                     LPKSDVLKFLLEDGSWIAIRPSGTEPKIKFYFGANSDNQEDVEFKLNNLISYILNVVD
                     SI"
     gene            complement(14745..16301)
                     /gene="murJ"
                     /locus_tag="PC471_001275"
     CDS             complement(14745..16301)
                     /gene="murJ"
                     /locus_tag="PC471_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HDF2788413.1"
                     /translation="MSKTAKAALWIMAATMFSKVLGFLRELVLANFYGTGMYADVFVL
                     TLNIPGLIIAVIGSAVATTYIPMYFETKKRLGDEGALKFTNNVLNICYIMAIVIAIIG
                     LLFTEQFVTVFAAGFRNDPAKFQAAILFTKIMISGVLFLSGSKIFSSFLQVNDSFVIP
                     GLIGIPYNIIIIAAIALSAGKNVWILPAGALLAMASQLLFQLPFAFKKSYKYKPYINL
                     KDESIKELVNLVLPMLVGVAVGQLNIFVDRLLATTLGDGKLSALNYANRLNEFVMALF
                     VTSIITVIYPKLAKMSGKDNKEGFISTIVKSSNCIILVVLPISIGAIILAEPLVRILF
                     QRGKFDALSTDLTSIALRLYSLGLLACGVRDVLYRAFYSLSDTKTPMINGSIALIINI
                     VLNLILIRPLGHAGIAISTSTSNIITVILLFISLKKKNGYFGGDKIIKTGLKSLVASG
                     VMAVATLLIYNNLYAFMGSGTIKEIISVGAGVLGGASVYTVLIVLFKVEEMDLAFEFL
                     RKGKQKLLRR"
     gene            complement(16416..17471)
                     /locus_tag="PC471_001276"
     CDS             complement(16416..17471)
                     /locus_tag="PC471_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp7"
                     /protein_id="HDF2788414.1"
                     /translation="MIKKISTILSLVLLISISSTIGVFADANPKRELIEGSIPEISTE
                     LNKRAFKDSKEVILVNEESIVDSISATPLAYAKNAPIVVTKSKNLGRVTRNYLKELGP
                     EKVTIVGGLKAVSKDAERNIEKMGMKVERIRGKDRYDTSLKIAREMYRTVGFDEAFLL
                     SSTTGLENAISVYSYAAKSGMPIIWAKDEGFEEQIDFLKGKNLKKIYALGDSKEFIAE
                     IDSNLKNIEGIKQINKSSTNVDLIKKFYDEKDIKKIYTARLDFGSRSDVNEYISLGVV
                     SAKENMPILICSDNLSRAQDKFLKDSNINDVVEVGYTVGDYSLFKSIFNLTFLSCIVL
                     ILLLLLITFRALRYESK"
     gene            complement(17566..18252)
                     /locus_tag="PC471_001277"
     CDS             complement(17566..18252)
                     /locus_tag="PC471_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HDF2788415.1"
                     /translation="MSGYTNDECEIPKIKSYPGADKEIASEIDYSIVKGTVLFDLYQR
                     IMDLVLSIIGLVIGLPLIAIFGILIKIEDKGPITYKQERLGKCGRRFYIYKLRSMRTD
                     AEKFGAQWAEKDDPRITKVGKFIRKTRIDEIPQLFNILKGDMGLIGPRPERPNFTVQF
                     NEEIPGFINRLAIKPGLTGWAQVNGGYEITPEEKLKEDIYYIKNRSILLDFKILFKTV
                     KVVLTGDGAR"
     gene            complement(18472..20496)
                     /locus_tag="PC471_001278"
     CDS             complement(18472..20496)
                     /locus_tag="PC471_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HDF2788416.1"
                     /translation="MKKTTKLLATGMLSVAMVAPNVALAAENTTANTESNSDININLQ
                     RKSVVLGSKSNASVKFKEKLNADSITLNFMCYDMPLEATLNYNEKTDSYEGVINYNKD
                     PEYLNVWELQSIKINGKDEQKVLNKEDLESMGLNLKDYDVTQEFIISDANSTKAVNEY
                     MRKTSAPVKKLAGATRFETAVEISKQGWKDGSSKVVIVNGELAADGITATPLASTYDA
                     PILLANKDDIPESTKAELKRLNPSDVIIIGDDGSVSQKAVSQIKSAVNVNVTRIGGVD
                     RHETSLLIAKEIDKYHDVNKIYIANGYAGEYDALNISSKAGEDQQPIILANKDSVPQG
                     TYNWLSSQGLEEAYYIGGSQSLSSKIIDQISKIAKNGTSKNRVSGADRHETNANVIKT
                     FYPDKELSAMLVAKSDIIVDSITAGPLAAKLKAPILITPKTYVSAYHSTNLSEKTAET
                     VYQIGDGMKDSVINSIASSLSKHNAPTEPDNSGSAAGKTVVIDPGHGGSDSGATSGLN
                     GGAQEKKYTLNTALATTEYLRSKGINVVMTRDTDKTMALGERTALSNTIKPDLFTSIH
                     YNASNGSGNGVEIYYKVKDKNGGTTKTAASNILKRILEKFNMKNRGIKTRTLDNGKDY
                     LYVLRNNNYPAILVECAFIDNKSDMDKLNTAEKVKTMGTQIGIGIEDTVK"
     gene            complement(20750..22033)
                     /locus_tag="PC471_001279"
     CDS             complement(20750..22033)
                     /locus_tag="PC471_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5009 domain-containing protein"
                     /protein_id="HDF2788417.1"
                     /translation="MGIGIECNKKINKSIDKTRIKKNNLNSNSNSNSNSNSNSNSNSN
                     SNRISNNAVDSKLMNSRIKSIDIIRGLSIALMIVCNNPGTWMRMYPQLRHAVWHGVTL
                     ADFAFPFFVISLGVTIPISINSKLKNNKSTLSIILSIFKRSILLILFGFFLNYLGNPD
                     LDTVRILGVLQRMGLVYFVTSLVYLLLKKLNVGSTATIITFLCISTFIIVGYYILAKP
                     YGFELEGSLAQLVDLHFFKGHLYKPEFEPDGFLTSIVAISSGMLGCTMGCVLLKENIG
                     EYKKFFKILVMSIILLIGAFIFNQYFPFNKRLWSSSFVLLMAGSYGILLSIFYFICDI
                     KNKSKIFTPIIALGSSPIFTYMCLEILSHVFWNVPKLTNKVDYPTTLVEWTTYELITP
                     WAGTTWDSLIFSLLYVLFWVIVMSIMYKKKIFIKI"
     gene            complement(22167..23744)
                     /locus_tag="PC471_001280"
     CDS             complement(22167..23744)
                     /locus_tag="PC471_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp5"
                     /protein_id="HDF2788418.1"
                     /translation="MKAPKTILTILTIALTLSSISIIPSYALTEEKLIGNGRYETAVK
                     ISQKAYSSSPNVVLVNDNSLADALSATPFAKAKGAPILLTESDKLDDRTEKEIKRLGA
                     KDIYLIGGTAVLNKDIENKLKSNGLNVERINGKDRYETSLILANKLKDIKDIKEVAVV
                     NGEKGLSDAVSVGAPAAQNKMPIILSSPKDGVEAFDKFIRDEKVIKAYVIGGTNSVSR
                     AVEKSLPNAERVSGKDRNETNAKVIEKFYTDTNLSNLYVTKDGSKNENQLIDSLAVGV
                     LAAKNESPVVLVGNKLNTKQRDILSTKKLNTITQVGGNGNEEAFDEIKSLQEKTVFEA
                     KTVEELTDMINIASPNDIINFKPKENTVNEAFRMVTNKPITVNIKGDCSKTLTVDMPN
                     GEVNNYATLVNVIVRNIGEGGFNNHDTITILSVRDKNGRVIENTRNSDIDTLMILASA
                     NDTKLINDGYIGKLIDNSSNSDITNNGTIDKKVNQVEDLEAKVDSIEKAIDSISQKVN
                     KIQDILDKLGFLKKFLS"
     gene            complement(24041..26452)
                     /locus_tag="PC471_001281"
     CDS             complement(24041..26452)
                     /locus_tag="PC471_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HDF2788419.1"
                     /translation="MRKYKLKKLSKLLVLLTVCFLIVSTIPVSAEKNKILTGVETAEY
                     SESYLQYLEDVKNGDIAKYNGVIPTPYEMEGTTLKTNVRSSLPASYKSSVSYDPRKLD
                     LTTPAKDQGKLNTCWAFSGMSTLEAYLKLKGYGTYDLSEEHFRWWSTGGIHGWNLTDM
                     TGSSNVTAIGYLTAWAGPKLEKDIPYNFKSEDEGATRPQNMDTAPTQFNVTDVVRISK
                     DKTSVKNAIMQYGAVTSGYAHYSAYLSDDENSYNCNDKSEPLNHSVSIVGWDDNYSKD
                     KFKPSVRPESNGAWLVKSSWGEFNSEKGFFWISYEDKTLLKDTDNYAMKSVSKPDSDK
                     KMYQLEYAGLSKIMSNKVTAANVFDFSRDSEKLDSVMFETDSVGAKYEVYYAPVVNGV
                     PQNNSMTKLASGTVSYSGYINVPTNSYSLPKGKGAIVVVIDNTANPNREKSTLAYETD
                     IDGYYLYEAKANLGESYILQNNKFEDINTYSEFSPCNFVIKAITKTSSGQATSGESLT
                     GADRYETAVKVSQKGWTSSQNAVLVNGDAIVDALTATPFTAAIDSPILLTGKDNLDSK
                     TKAELQRLGTKKVYLIGGENSLSKNVQTQLSNMGISVERISGSDRYKTSISLAQKLNS
                     IKSVSQVAVANGVNGLADAISVGAAAADNNMPIILTNEKSELQGADEFLNSSKITKSY
                     IIGGTATLSSNLESKLSNPTRLAGSNRNETNAKIIDKFYPSSDLKYAFVVKDGSKSQG
                     DLIDGLAVGALGAKTDSPVVLVGNKLDESQKNVLKSKKIETPIRVGGNGNENAFNELN
                     TLLGK"
     gene            26642..27043
                     /locus_tag="PC471_001282"
     CDS             26642..27043
                     /locus_tag="PC471_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HDF2788420.1"
                     /translation="MILKKHLETILYLFFGALTVLINIITYLFFTRIINFTFLLSNSL
                     SWSIAVLFSYITNKFFVFKSEKTEFLFLLKEFVSFLSCRLFSGFIEILLMYLMIDLFS
                     LNDILVKIGTNIIVIILNFVFSKVVVFKHRN"
     gene            complement(27108..29225)
                     /locus_tag="PC471_001283"
     CDS             complement(27108..29225)
                     /locus_tag="PC471_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HDF2788421.1"
                     /translation="MQISKKIISLLTAAFLSIVLPSNVVNASNKDSLIGSGRWETAIK
                     ISQNGWLRSDNAVLVNDNSIADALSATPFAKLKDAPILLSGSKELDFRTKSELKRLGV
                     KNVFLVGGSNALGVNVENELKSEKITVERIFGNDRYETSLKLAEKLNNEKDISDIIVV
                     NGEKGLADAVSVGSIAAQNYMPIILSDSNLGVEVANKFIKAQNIKNSYIIGGLNSISN
                     EIEKNLPNSKRISGNNRNETNANVIKEFYKETDLRNAYITKDGMKKQSDLIDSLSVGV
                     LAAKNNSPVVLVGNKIDSIQKDVLNTKIFSTVTQVGGIGNENVYKDILDMQEETKYTV
                     ETIEELDSALKKVDANDIIKFRPDKKISESFKLETEKAIKVDLYGEYSQSITINMPNG
                     ELNNTGDISKTLILKDIKDGTLTNSGYINQMDISDDNGCKIENENNGQIWIVTIFSSA
                     KDVNIRNDGTITKIDNDSSSTNIRNSGKIDTVSGNKEPGISGNKPNTNNTDKDDDSDT
                     AAGLSPKISACSPLENNQIMLTVPQKPKNSNYKIYYRVLSYKPSAMRVGDRININDWD
                     LVEDTEPLKVNAKNGSYIEVVEIDYTRKKVSRWGRAGATDDGFKIVANGIKSTVSVDE
                     NSKVTISVTGYNGNKVYYRIKTTKPEAMNVGDEIDSSWKSTSNSDIILEGNKVNGLYV
                     ELASGENNKVEKWGATDKIEIVS"
     gene            complement(29256..29963)
                     /locus_tag="PC471_001284"
     CDS             complement(29256..29963)
                     /locus_tag="PC471_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HDF2788422.1"
                     /translation="MVDATLFFTPHQDDETLSMGSAIIEHVEKSDTHVILCTDGSKSI
                     IRKVLDDGGSCSYHIKDVHKYSLSESEFSKDRDEEFKDSCEAMGVKESNIHIEDNRAH
                     DGELSKEKAKEIILKYLEEYPEAKVKTVTPFKASGIHEDHRALGEAALELYREGKIKD
                     LRFYVEPYDYKDFKKVNPNVEVWKVLPSQEEKLLSAMNAYKKWNPESGHYAIGYHSVK
                     SHFDDLELNKTQYVHAP"
     gene            complement(30070..31941)
                     /locus_tag="PC471_001285"
     CDS             complement(30070..31941)
                     /locus_tag="PC471_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp2"
                     /protein_id="HDF2788423.1"
                     /translation="MNKKNLSVIMAAAMISTSVAPVFAAETTQVKKETITKKEATELV
                     SKVRGLMSQKYTGGSQVGQPIYEIKVGETLSKLKIITNIDELEKLVNALGENKELIVT
                     ITDKGHITNSANEVVAEATEKYENSADLSAEANSITEKAKTETNGIYKVADVKASYDS
                     AKDKLVITLRDKTETVTSKTIYVGIGDEKVDLTANPVDSTGTNLDPSAEGFRVNKIDK
                     LGVAGAKNIDDVQLAEITIKNSDLNTVSPQDLYDGYRLTVKGNMVANGTSKSISDISA
                     KDSETGKYKFTIKYTDASGKATELTVESTNEKDLKDAKAALEGNSKVKLIAGDDRYAT
                     AVAIAKQTKYTDNIVIVNSNKLVDGLAATPLAQSKKAPILLASDNEIPKVTLDYIKDI
                     IKKSPSAKIYIVGGESAVSNTAKKQLESVTKNVERLAGDDRHMTSVAVAKAMGSFKDA
                     FVVGAKGEADAMSIAAKAAELKAPIIVNGWNDLSADAIKLMDGKEIGIVGGSNNVSSQ
                     IENQLADIDKDRKVQRVEGETRHDTNAKVIETYYGKLDKLYIAKDGYGNNGMLVDALA
                     AGPLAAGKGPILLAKTDITDSQKNALSKKLNLGAEVTQIGNGVELTVIQKIAKILGW"
     gene            complement(32285..34630)
                     /gene="secA"
                     /locus_tag="PC471_001286"
     CDS             complement(32285..34630)
                     /gene="secA"
                     /locus_tag="PC471_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HDF2788424.1"
                     /translation="MSVLDTILDKADEQEIRRLNVIVDKIESLEKDIENMSDDELKKM
                     TDLFRDRLKKGETLDSLLPEAFAVAREASKRVLGMRQYRVQLIGGIVIHQGKIAEMKT
                     GEGKTLVEVAPVYLNALTGKGVHVVTVNDYLAQRDKELMQPVYEFLGMSVGVIIADLI
                     PEVRKVQYNCDITYGTNNEFGFDYLKDNMVTKKFDKVQRELNFAIVDEVDSILIDEAR
                     TPLIIAGEGDEDSKLYELANSFIKTIKEEDYELDRKDKTVALKESGIDKAESFFGIKN
                     LTDIENIELYHHINQALRGHKLMEKDVDYVVVNQEVMIVDEFTGRVMDGRRYTDGLHQ
                     AIEAKEGVEIKDESKTMATITFQNFFRLYNKLSGMTGTAKTEEGEFESIYKLNVVQIP
                     TNKPIIRADLHDKVFRTEKEKYQAVVEEVIRIHKTKQPILIGTVSVEKSEMLSGMLKK
                     EGIKHQVLNAKENDKEALIVSKAGKLGSVTIATNMAGRGTDIALGAGDKEEESRVKEL
                     GGLYVIGTERHESRRIDNQLRGRSGRQGDPGTSRFFVSLEDDVIKLYGGKSIEKLMKR
                     PNSNKNNSIESKSLTKAIERAQKGVEGKNFELRKNVLKYDDTINEQRKVIYNERNKVL
                     NDEDIQVDIQNMVKDIIKEAAAEYLTKRKRDYYGYFKHLYNTFMPADTLLIPDLDNKS
                     IEDIIDSTYEISKRVYDLKKMVVGLDKITEMEKIVLLKVVDQYWVEHIDAMEQLKQYV
                     GFKSYAQKDPFKEYAMEGYEMFESLNKNIRETTIQYLYKFN"
     gene            complement(34866..37046)
                     /gene="slpA"
                     /locus_tag="PC471_001287"
     CDS             complement(34866..37046)
                     /gene="slpA"
                     /locus_tag="PC471_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer protein SlpA"
                     /protein_id="HDF2788425.1"
                     /translation="MNKKNLAVVMSAVTVIGSAAPVFAAAEDSFPNGNTTVSSSKYSD
                     IERILKKYVADGVTGITVNFFDKKGNPKGVSISSLEASQISYVKDEIKDLKAGEYAKI
                     SVKITDGTEKKYDDTLLKELDSNAVVTKVGTATTVKIVDQDGNSIENDAKLKLQKYDT
                     TTSAYVDGATKDEVLAATKAAVKTSKFTDKTGNNATGTVAITATYADAVAGAIKLKYD
                     TQAAVAPTLDTAVASLDGTKEIYDFSKPVITVNTTDGTSKLSFEKTGEKVGAVESNVT
                     VIAASDETVTISGDAKEKAEALAKKYVFKDTELEDAYKTVTASDFEKTDNDYYEVVLY
                     PTGKRLNTASTYASSNYKPELPTSDRVDTPAIITLRSTNKNNLKSALDELRTYNNSYS
                     NNSVLAGDDRIETAIEISKDSYNADGGIKGDYVEANEVVLVGSQSIVDGLVASPLAAV
                     KDAPLLLTSKDKLDSSVKSEIKRVMGLDDKTGITSKKTVYIAGGENSVSKEVANELKD
                     MGLKVERLSGDDRYATSLEIADEIGLNHNKVFVVGGTGLADAMSIASVASNKEMPIVV
                     VDGKGKDLSTDAKDFIGSAYVDIIGGKSSVSEDMEDAIDDATGKSPERVSGDDRQDTN
                     AEVIKTYFEKDNSDSVISTGVKNFYVAKDGSTKEDQLVDALAIAAVAGHNEAPIVLAT
                     DSLSSDQSVAISKVTNSDDSKKLTQVGKGIADSVIKRIKDLLEL"
     gene            complement(37339..38937)
                     /locus_tag="PC471_001288"
     CDS             complement(37339..38937)
                     /locus_tag="PC471_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HDF2788426.1"
                     /translation="MKVNKRVLSIGLTISLIMAGAPNINALSSIEKIQGKDRYETSAL
                     IADKQIYDTVILVNTDNSIVDGLSASGLSGVVKAPIMLVQRDKIPTDVEKRLKDVKNA
                     YVIGTEDTIGKSVENQLKNKGIEVKRIGGEDRIKTSYLIAKEISAIKPVNDGDKVFLV
                     NGYTGEADAMSVSSVAARDGVPVILTDGKSIPFKVDGVQCYSLGSEEIMSNELVSKTN
                     SVRIAGKDRFETNKKVIQRFYKGTKKFYVSQGYKLVDAVAGSPLAKDKPIVLVNDGSD
                     KSVLRGADEVTSLGGMDKKVADQCISSASDKNTMPTITANDVEISVGDKFDNSMLNIV
                     ATDYYGNNLKPNIEGTVDTSKAGTYVLNVSAFDNFGQKCEINVNVKVIINTSTKDPNS
                     YEFKAMVSNEMYNLVNSYRKEKGKKSLRELDSLAGMANAWSKYMDEKKIFAHEIDGKN
                     AAEVFSGFGMRTDENIAYLPMNVKSVYTSKDAKEIAKSVFDLWKKSSKYNDNMLKEEF
                     YSFGFGMYVSNQREVNATMEFLNS"
     gene            complement(39156..40757)
                     /locus_tag="PC471_001289"
     CDS             complement(39156..40757)
                     /locus_tag="PC471_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp11"
                     /protein_id="HDF2788427.1"
                     /translation="MKMNKKILSLGLAVSLILVNFKSVNASSVVEKIYGKDRYETAAK
                     IADKQTYETVILVNTEKSLADGLSASGLSGATKAPILFTQQNKIPADTNRCLKNIKKA
                     YIIGTEDTISKSVEKELDSKNIEVKRIGGEDRLKTSYLIAKEIATIKKVDKVLLTNAY
                     SGEADAMSVSSVATRDGAPIILTDGKSVPFDVKNIQSYCIGSEEIMSNPLVKNTNSVR
                     IEGTDRFETNKNVIDYFFNSADGFYVSDGYQLVDAIAAAPLTKNSPMVLVNDGSDKTV
                     LEGAKNITSVGEIDEKVIQQCISASKSNGQPPAITVGSTEVYKGEKFDTGKLNIVAKD
                     NTGKVLPIEVDGFIDTNRVGTYILTLKATDEWGKSTGKRVEIKVLDDKSHDYNSPEFK
                     KMVSTEMYNLINSYRKEKGKEPLVVSSKLEGMANAWSKYMMDNKVFEHYIDGKNAPQV
                     FSEFGMRSEENIAYIYIDSKSVQTTQDAKDLAKAIFEVWKKSPEYNANMLSDEFYSTG
                     FGLYILSDGQVHATQEFLNGNEGSL"
BASE COUNT        12395 a         7614 c         4312 g        16535 t
ORIGIN      
        1 ttctttgctg tttttataac gttcatcact attttaatat tactaaactc ttaaatctct
       61 atgtaaaaca ttaaatgtaa ttatatattt aatgttaata ttcaatccta tgtaaatcaa
      121 tatctattat tattgtaaaa cacgtgtagc agttataagt cttcttgcat aattagttcc
      181 tggagccatg ctagtaactt ttactacatc tcctgtttgt ggtgaatgta tgaatttaga
      241 atctcctaca tacaatccaa cgtgatttat tgaaccacct gaaccaaaga aaactaaatc
      301 tcctgatttt aattctgctc ttgatacttt ctttcctact gttgcttgtg atcttgatac
      361 tcttggtaaa ttaactcctg ctccattttt atagacataa gaagtgaacc cagaacaatc
      421 aaaagaattt ggtccagttg ctccccattt atatggctta ccttgttgct taaatgctaa
      481 gtttaaaaca ttttgtacct ttgcatctac atttccacct tgaatagtta aataatctcc
      541 atttacccat cctgtaacat tgtcaacact ttttatttta atccatccat ttgaatgtaa
      601 ttctaaaact tcaactttgt ctcctgtgtt tatctttcct actactgcat catttgcact
      661 agctccactt cttacattta aaaaatcaca ctggtctact gttgcactca taggtgaatt
      721 agttggctct gctgcactag ccgttattgc tcctgcttct actgtgacaa ctcctgctat
      781 taacaccata gctataaatt ttttcaatat atgtttttta tttttaatat tcatcataat
      841 ctcaaatacc taatcctttc gaaaatttaa cattttgttc cttcgtttcc aattcctaat
      901 taacattcta tcttacatca aaataaattg caaccttttt tgtaatcttt ttgtaatctt
      961 tttgtaatct tttgtttctt tttagtatat atttgtttct ttttgctccc cctggttaca
     1021 aattagaaat atattctact tttctctagt tgttgcaatt tctttgatat tagcatcttc
     1081 tggtttaata taagttggca ctaattccct cataatacca tctatttttt caacctcttc
     1141 attgttagta atttctctta aaagttttaa gtattttgtt attttttctc tatcaacatc
     1201 tattggtcta ccaataaaaa tctttttatg ctctgtatcc aacagacctt cttctgacat
     1261 aagtagttct tcatacaact tttctcctgg tctaagacca gaaaattcaa tttttatatc
     1321 aacatctggc tcaaatccac tgaatttaat taagtttttt gctaaatcca atattcttac
     1381 aggttctccc atatcaagta caaatatttc tccaccttta gccatagccc cagcttgtat
     1441 aactaagcct actgcttctg gtattgtcat aaaatatcta ataatatctg gatgagtaac
     1501 tgtcactggt ccgccatctt caatttgctt cttaaaaagt ggaattacac taccattact
     1561 accaagcaca tttccaaatc taacagcaac aaattcagtc tggctttcag cattcatagt
     1621 ctgtataatc atttcagcag ctcttttagt agctcccatt atgttagtag ggtttacagc
     1681 cttatctgta gatataagga caaatttctt agctctatat tttgaagaca agccagctat
     1741 gtttaaagtt ccaaatatat tattctttat agcttcgcca gggctcgatt ccataagagg
     1801 aacatgttta tgtgctgctg catgaaatac cacttctggt ttatatttat taaatatttc
     1861 atccattctt ttttcttctc ttattgatgc tattactgtt tttaaatcta atttattttt
     1921 atattttcta agcaattctt gttgtattga gtaagcatta ttttcataat tatcaactat
     1981 aataagctct ttaggattaa atcctgcaat ctgtctgcaa agctctgaac caattgagcc
     2041 tcctcctcca gttattagga ttacttttcc ctcaatgtaa ttacttatct ctcttaaatt
     2101 tgtttttata ggctctctac ccaacaagtc ttctatctct acttctctaa tctgttttat
     2161 atcaactttt ccatctataa tttcgtaaat tcctggtatt gtctttattt tacagttagt
     2221 gtttttacat atatcaataa tctctttttt tctacgtttc tctatgtttg ctattgaaaa
     2281 gattatctca tctacattgt acttttctac cacatctttt atatctgaag ttgttcctac
     2341 aattttcaca ttgtggatta ttcttccaat tttattttta tcatcatcaa ttatagccac
     2401 tgctactttt ttaagttctg gatttctttt tagttcctgt ataaccattt ctcctgcatc
     2461 tcctgcacca attataagga tatttgaaac ctttcctcta gaatatgtct tgttcatgac
     2521 tattcttata gctctatagg ctattcttgc gccacttgta aacattataa taaatattgt
     2581 atttaataca taaaacatta taggataatc aaaccctaat aatttattta ttatataaat
     2641 cggaattaaa taagctaaag ttgccacaaa aatagatata atttcttctt ctcctgcata
     2701 tctccataat gtgttgtagc acttaaacaa tctaaatgat actatatgaa gtactatata
     2761 tatacacgct gaaattatta tatcattagt ggtaaatgca tttaaattac ctgtcaagct
     2821 taaagacatt cgaaacgcta ctagtacgct acaaatatct agaattaata gtgttaaatg
     2881 tctaaattta tattttttaa aaagcctatt aaaaaagctt tctttctcta aatctatact
     2941 ttctcgcata actagaagcc ctcctttaaa cagcaaaagc tacgcaccta agcatagcca
     3001 acaacatcta tcttttgctg tattttttca ttttattttt tatatttatc aaatatagaa
     3061 tcataaattt tttctatatc attaaagttt aaattaacct cataattttc tctaacaaat
     3121 tgacgccctt tcttacccat attaagtcta agttcatcat tttccattag ttttattata
     3181 gcatttttta tttcttctgg ttgttttggt tctacaatta agccagtttc attttcccat
     3241 acagattctg gatgtcctcc tacatttgta actataacag gtacttcaca tgcttctgat
     3301 tctatagctg ccactccaaa tccctctctt aaagaaggaa atacagttac atcaaaagaa
     3361 ttaaaagtct tgcttacatt ttctggagat attcttccta aaaactgaac atcattttct
     3421 atacccaatt ctttagctaa atttattagg ttgtccatct gacttccaga gccacctatt
     3481 ttaagaatta ttttcttatc tttatattca tctttaagca tcttaaatgc ttgtattaaa
     3541 tattctattc catatttttt ttctaaactc tttataattc ctattacaaa agcgtcttct
     3601 ttttctactt ccataggctt aaacctatcc atatctactc caaaaaaggt tacatctaca
     3661 tgtttagctg tatatttatt agcctcttta gccatatctc tactatttga aaagatatag
     3721 tctgcttttc taaagttatg ttttataatt tgtttttgta taaaaccatt attagggaaa
     3781 tcataaatat cagtacccca aacagaaata atatatggat gatacccaag tagacttcct
     3841 ataaatccat aactacttgc atactgtgca tgcaatatat ctggctttat ttcatttact
     3901 agcttcttta tctgctttat tgaacctaag tatcccgttt ttttagaaat attttcattt
     3961 tttagttctt ctacattaga accaaaatta taaattttta ttccttccat gtgacctcca
     4021 tttaaagaga ttacatgaat gtcatatcct ttatttttaa aaaaactaca ccattttaaa
     4081 gtatgaggat ttgacccatc agctaaataa caaacagtct tcataatatt taccttccta
     4141 tcgaataata ctcaattcct aactcttcca tatctttaat tttataacaa tttcttccat
     4201 caaggactaa aggatttttc atcttttcct tatacttatg tatatcataa tttaatacat
     4261 ctttccattc tgtcattata aaacatatct ctgcatcttc tatcgcatca tctacagaat
     4321 cagcataaag tatagagtca ccaaatattt tcttagtatt atttacacca attgggtcgt
     4381 atacagtaac tcttgcccct ctttcgagca aatattttat atttggtata gatggtgctt
     4441 ctcttatatc atcagtgtct ggcttaaaag tcaaacctaa aacagcaacc ttcatatctt
     4501 gaaagctctc tactaactta gatgcctttc taactaattt aagtttttga ctttcattaa
     4561 cttctattgc agcttttact gtttttaatt cataaccatt atgttcagat aaccagtgaa
     4621 gagcttttgt gtcttttgga aagcaagaac caccatatcc tattcccgcc tttaaaaact
     4681 tacttcctat tctatcatca taactcattc ctaaagctac atcttctatg ttagctcctg
     4741 ctatttcaca aagatttgca atatcattca taaatgatat tttaagagct aagaagttat
     4801 ttgatgcgta ttttatcatc tcagcactat ttctatttgt aactattata ggttgattaa
     4861 aaggcttata gatttcctct aatatttctc tagcccattc actttctaca cctataacga
     4921 ttcttgaagc atgtagagta tcttttactg ctgttccttg tgataaaaac tctggattag
     4981 atgcaagttc tacttttaca tcatttttta ggtttctatt tatataatct tctatctctt
     5041 catttgtacc aattggaact gtagatttta ttactactag agtatctttt gtcacagttt
     5101 cagctatttg ctttgcaact gcctttatat aatctaggtt ggctgaacca tcacttctct
     5161 caggtgtacc tacagctatt aatattacat ctacatcttc ataagctttt ttatagtctg
     5221 tagtgtaatc tattcttcct ctgttgtaat ttttaaccat aagttcttct aggccttctt
     5281 catatatagg tgctattcca agtttcattg atttaacttt ttgttcatct acatcaacac
     5341 agactacatt ttcattgcct acctctgcta gacaaacacc tgtaaccaaa cctacatatc
     5401 ccgtcccagc tatagctatt ttcatttttt tacctccaag tatataaaac ttatactctt
     5461 tttttaatgg cattaaaaac atagaaacaa aatacatata gactttttgg taaacttaaa
     5521 ttttcaacat ttctataagt attccaagtt cgttttaatg cttttatctt gttacttgat
     5581 atagaatttc caactaatct gtaattcact aaatcttctt gaatcccata tgcatatttt
     5641 tcctttttaa gtaactgtaa ccaggttgca gtatcttgtc ctcttcttac caaaggcatg
     5701 ctgaactctc ctacaatctc cttatcaatg accactgtag aacatcctat tatagtattt
     5761 ctaagtaatc cttcatagtc aattttctta ggagctcttg caaccttatt cgtttttgac
     5821 ccatcttgtc tcatatatct ataagatgta aaagaaaaac ctacattttc ttttaacata
     5881 tattctatct gaatctctaa ttttctacta tcccataaat catcactatc tacaaatgca
     5941 ataaatcgac cttccgcatt cttaatacca acatttctag atactgcagc tccagaattt
     6001 ttttcaagct ttatatattt tattctagca tcttcttttc gaaaactttt tataatttca
     6061 cttgaattgt ccttagaaca atcatccact aataaaagct gccaattttt ataagtttga
     6121 ttttggattg actttatcgt ctctgataaa aactcttctg aattataaac aggagtaatt
     6181 atcgatacta aaggctcatt catattttta ctctccttat atagcaacta gcagtactat
     6241 accaacaagt ttacttagat actaattttc tttgtccaat atatttgtaa tatcctaatg
     6301 ggaataatgc taaatacaac aatttatcag atacttttct taattcacca aaataatgac
     6361 tatcagacaa caatgagtat ctggctaaat ttatgtaatt ttttatccac atatatggat
     6421 atctacttaa tggatatgta ttttgattta ttaaatataa aaactctaat gctctacctc
     6481 ttaatgcatt tttacttaat atattcttag tcaaaccatc atccaaataa tctcttatta
     6541 tcataggttt atttacacat ataactttat agtatttaga catttcattc catactacac
     6601 tttctggtaa aaatctcact tcttttcttt ctggaaaagg aaattttata agctcatcag
     6661 ttacaaatac tatgctttta tctcccttaa cgccatactt aaaatatagg tctgcaaagt
     6721 gacatatttt cttatcttca ggcatttctg tccctatcaa atctccactt ggatatacac
     6781 aaagtccaac aactccacta aatccttctt tattctctaa agaatcccac tcatcattag
     6841 caatttcaag agaatcttta attaaaccat catcactatc tacaatgaaa aatagctctc
     6901 catctgcatt ttttacacct agatttattg cagtatgttt tcctccattt tctttgtata
     6961 tatatcttat atttaattta ttttctgata taaatttttc aactaactct ttagtactgt
     7021 cgctacttcc atcatcaaca atcaaccatt caaaatcatt gaaatcataa gtttgagctt
     7081 ttaaatcatt gtacaaattt tcaagtaaat gagctctgtt atacgtcgga gtaaatacag
     7141 taaatttata tttatacatt aattaaatct tcctctctgt ctaacttttt ttgattccgt
     7201 ttattccata attaataaag tagtatatag ctttgtataa aggtattttt tctatatttc
     7261 tatatatatt ccaagtccac atagcagctt ttattttatt accacttagg gaattactag
     7321 attttctata taatgctaga acttcattta ttccatgagc aatatgccct tttttcaata
     7381 tcgataacca taaaacataa tcttcatgtc ttacaccact cattcttatt tcaaaatcca
     7441 atttagactt atcaatcaca acagtaagac atcctaagat atttcctttt aataatcttc
     7501 tataatctac attaggtgga acttttatta ctttgtttaa ttttttatca ttttcatcca
     7561 tcagttcata tgaagtaaat gaaattacat agtcattttc taacataaaa tttacttgtt
     7621 tttctaactt actactattc cattggtcat cactatctaa aaaagctata aattgtcctg
     7681 ttgccttact tagtgctaaa tttctagcat tagagacacc cttattagtc tcagtcttta
     7741 tacattttat tctactatcc tgttgcatat aagatttgac tatattagga ctattatctg
     7801 ttgagcaatc atcaataatt aacatttccc attcttgata agtttggttt aatactgatt
     7861 ttatggttgc ttcaataaat ttttcagaat tatacatggg agtaattata gatactaaat
     7921 tcttcttcat attaccttcc tcatcattta taaaatattt atttaatttt acttaataca
     7981 caatcatata tcctcttgca gttcttatta tcattatatc tgaaaaactt atttctttca
     8041 tttttttggt tatccataac ctcaaaatta ttttcaataa tatcaataag tttattcacg
     8101 gcttcatcat tattataagc caaagaccca aacatttcac tttttaaatc tatatatgaa
     8161 tcaacttttt ccatatattc atccaaatcg aattggtaaa acaaaatagg tctattcata
     8221 tacaaaaagt ctatggctac acttgaataa tcagttatat ttgcagctga ttttctgatt
     8281 tcatttgcta gagttacccc tttatctaaa aacacaattc gttttcctgt tatattttct
     8341 tttatatcat ctataaactc atgcataaga tgatgcatat aaatttttac atttatatct
     8401 ttctcatcta atattttatt taatttgtca tcatttaaaa aattcattat atttttaaaa
     8461 taatctgtat ccgtaaattt tcctgaacta aattttatcc aatctctcca tgtaggagta
     8521 tataatatat ctcgagtttg tgcaacttca tttgttggta aattgtcata tcttgcattt
     8581 ccaattatat aaactgagtc atctaacatt ccccaatcat ttaccataac atttctttca
     8641 aattcagaac ttgctgtaac tatattatag cttttatata ggttatctat aatatttttg
     8701 aactttttgt tcataggttt tcttttttta aaacttatag tcccatgatt taaaaacact
     8761 tttatcgttt ttttatgaaa ataattaaga acaggtacag caaaattgta tggtgctata
     8821 tctgctgatg gtgcatgtga aaatactata gcttttgaat tgtaataata cagatagttt
     8881 tctacacttc ctcttattaa ttttttgcct ggaattttat caaatacttt actgtcttta
     8941 tttactaccc aataagtttc tacatcatta tgttctttta gcatatactc ataaaaaaac
     9001 tttgaattat cattgtatat atcccctgaa tgtccaccta taagccagat atctctacca
     9061 ttaaacttat gcttattaaa tggatatata aaaaaagcca aaagcatatt tattaaaaat
     9121 ttaaccttgt ccataaacac catcctcatt ataacttgta ctgtctaaat ccattaattc
     9181 ttccttttat agtggcacta tgataatctc tattggaact caatataaga cctcctatat
     9241 ttcttacaag ttttgatacc ataacttttg caaacttata attttttcta tagactattt
     9301 cctttttgca aagcgctcca aagcctcttc catagttata atctttttga tacttttctt
     9361 tagatttaga atgttttttt gcaggatggt aaattatatc atttccaaag tattttcctt
     9421 taaaacctaa actcaataag tttaaaacat aatctatctc ttcaccagca ccaaactctc
     9481 caccaacacc caatttctca tcaaatcttg tatagtcttt accttcaaaa tcaataaaaa
     9541 aagttatgga tgttatagta tccaaaacgt tggaacttgt gatatcacaa gttccatcat
     9601 acattttctt aaatgcatca actttatttg aatccatagt tttgcaactg tatattttat
     9661 aatctttatt tttattaaag aaatttataa ctttttctaa tgtatcattt tcgtagacac
     9721 aatcatcatc agggaaacct ataatttgcc ctacagcatt gtctatgcct acatttctgt
     9781 tataactcaa tcctttttta gagcttttta tatactttat atctaactta tcaatgtatt
     9841 tatcaactat ttctttaact ttgctattat cattttggtc tactactata agttcaaagt
     9901 ttttgtaagt ttgattttct aagctgtcca taagtaagta tatatcatca tatctattta
     9961 atgttggcat tattaatgat attaacatta ctacctccta cttttcatct tccacaaatc
    10021 tatttccctt ttatcattta atatcacttc ttttattagt aatactactg caaaactaaa
    10081 tgaagcttct aataataaat tttctgcaaa agagtatagt aaaaatataa ataaagctac
    10141 taaatatgct tttttatctt tttctataaa gctttctatt cctttataca tcaaatacat
    10201 aaataatata caagcaacta ctcctagtac actaattata tatacataag aactatctaa
    10261 tggattagtt gcttttatag caggactata tgaatttcca aaaggtttta gaaaattccc
    10321 cttttcagcc aagtatacat gccagaactt aggtctactt gctaataatc tatttaataa
    10381 agtgctttta tcaaatactg taccaataat tacactaaat aaagtaagta ttatcggtaa
    10441 tgtctttagg agcattggta gtccttttat tttttttata tctatccatc ttattatttc
    10501 tacaaatatt aatcctgcta atatagtgaa aaatccagtt ctactatatg tggtttgata
    10561 aacaaaaaat gcagaaccaa ataaaattat tctatcccat ttattataat ctttatatct
    10621 taagaatata tatgctgcat atatagtaac catatgataa aatacactat ttggattacc
    10681 aagtccaaaa tccattcttg taacaagcat atctattttt tctatatatc tatagtggac
    10741 ttcctgatta aatatcaatg aaggaaatag attaaataat aatatagcta ccattaaaaa
    10801 acatgctgat attattaaat aatttttaac tataaattta tcatctattt gcttagctga
    10861 tacagctaat aaaacgatta ataatatagc tccttcccta tttaataaat aaagtaagac
    10921 acaaactcca actagtacca tttctgttat actttttctc ttgtcaaata aaaaaagtag
    10981 cattagtatt cctattgaag aataaaataa cgctgattta atcatcccta tatccatatt
    11041 tgggtcaata aatgatatca tatactttct aagtaatatg ataaacaaaa caaatatata
    11101 aagatatttt ctaatcataa tatttctatc atcatttaaa gtcttcattt tactttctcc
    11161 cttttagctt cgtaaagtat tacttgcaaa tattttacaa cacttccata tcttatttac
    11221 acctgttaag tgtttgcttt ctaagaaata atttctcttt ttagcccaac tttttcccca
    11281 ccaatgtatc ccaaaagtgt tttctgttat acattctggt gtaaagtctt ctttaaatcc
    11341 aaaaggataa aaatattctt tgtcgtaaac acgtattcct tcttcataat tatttgggtc
    11401 aatcttctta taattctttt ccataagatg agtcattatc tttggtattg taaacaatga
    11461 agaagtaagt acttcttttt cataatactc caataagtct tttaaaaaag gatgtccttt
    11521 ttctacacct attatagcac catttatata ctctctatca tcttcatacc cacagattag
    11581 cctattattt tctaaaagag gagttatatc ctttaatatt tgcatatctg tatccatata
    11641 aactccacct tgttcataga gtacttttat tcttgtataa tcagatatga atgcccaaag
    11701 ttttctttta taacattctt ccaaaaactt atttcctttt atttctttct ctatatcaaa
    11761 gtttttttca ttccactcta ctatctcata ttctggaagc ttttctttcc aagaatttat
    11821 acatatattt tctatatttc cttttggacc accaaaccat acataatgta tttttttagg
    11881 aatcaaatga acacctccta catatattct tgtctatctt cttcttttaa ctgctctact
    11941 atttttttga catcttgagc tttatctttt ctacaaacca taactgcatc atttgtagat
    12001 actactatca aatcactaat acctattgtt gatattaatt tgtcatctga atatataatt
    12061 gaatttttag tatctatagt aatattttca cctcttttga tattaccttc atcatcagtt
    12121 ggatatattg cacctagaga atcccaagaa ccaacatcat tccatccaaa atcaccagga
    12181 actactataa catcattact tctttccata ataccataat ctatagagat tgattgtata
    12241 gttgggtata tttcttttat tttttccatt tcttcttttg ttcctaagta tttacttata
    12301 tcttctaatt tttcatatac ttttggcaag tatctcttaa aatcttctag tattttagat
    12361 accttccata cgaacattcc actattccat acatactttt tagagtttac atattcttta
    12421 gcaatttcat agtttggttt ttctacaaac tccactacct catatgcaac atcttctata
    12481 gtattttctc tattaaaatt tatgtatcca tatcctgttg atggaaatgt aggagttatc
    12541 ccaatagtta ctagtttatc attattttct gctatataga tagctttttc caaaatagat
    12601 ttaaattctt tttcatcttt tatataatgg tcagctggat atacacacat aacaccatca
    12661 ccatattttt tcattatgtt aaatgctgca aaacctatag ctgctgctgt atttctggca
    12721 catggctctg gcaatatatt actatccaaa catttatctt ttactatatc ctttaaagct
    12781 tccaattgct tctcattggt tactataaat aaatcatctt tttttgctag agaatctatt
    12841 ctgtttatag tttcgtttat taaagcatct tctccactta aatttataag ctgtttaggt
    12901 acttcttgtc tactaagtgg ccagaaacga gttcctcctc ctccagccat tatgacattg
    12961 tatactttca ttatttatct ccccttagct ttttatatag aatccactac atttaatata
    13021 taagatatta aattgttcaa cttaaattct acatcttctt gattgtctga atttgctcca
    13081 aagtaaaatt taattttagg ttctgtacct gaaggtctta tagctatcca agagccatct
    13141 tcaagtaaaa atttaagtac atctgactta ggtaaatcat caacaccatc tttatagtct
    13201 aaaattttat ctacttttac atcagccaca ttatctattt taacacttct aaagtaaagc
    13261 atcatctctt ttattttttt tattccatct atgcctttta aagttactga ctttaagtcc
    13321 tctttgtagt atccaaattt attatatgta tctatcaaag cttcatacaa gttcatacct
    13381 tttgaagaat agtatgctgc catttcacat atcataagtg aagatacaac accatcttta
    13441 tctctagcat gagttccaat taagtaacca tagctttcct catatcccat tataaaactt
    13501 ctatcattgc tttcttcaaa cgcttttatt ttttctccta taaatttaaa tcctgttaaa
    13561 gtatttaaac aatctacatt ataagccttt gctatttttg cacctagttc tgatgtaact
    13621 atagttttta ttattgtagg attgttttta ggaagtttat tttcttcaac taaactctct
    13681 aatatatatc taactaaaag tgaaccgact tgatttccat ttaagactac atattcacca
    13741 cttgaatctt ttacaacgat acctactcta tcacaatcag ggtctgttcc tattatcaaa
    13801 tcagtgccat tgttctttgc catttcaata gctatattaa acactgactt ttcttcagga
    13861 ttaggatact ttactgttga aaaattagaa tctggcaatt cttgctcctt aactaccatt
    13921 acattttcaa agccacattc atttaagact tttcttactg gcttatttcc tgtaccatga
    13981 ataggtgtat atattatttt taattttttc ccgtattcat ctattatatt ttgtcttaaa
    14041 acttgcttct taacagcttt tataaactca tcatctactt cattatctaa tatagttatt
    14101 aaatttttag ataaagcttc ttcaaaatct atacttttaa tagtactgta atcatcaatc
    14161 ttattaactt ctgctattat atcatttgcc atatctatac agatttgacc accatctgag
    14221 tcatatactt tatatccatt gtattcttta ggattatgac ttgctgttat tactattccc
    14281 atagcacact ttaaatatct aacagcaaaa gaaagttctg gtgtaggctt taattcttca
    14341 aaaatataag cctttacacc ataagcactt aaagtctttg ctacttctat acaaaactcc
    14401 ctagacatgt atctactatc atgtgctata actattccct tttgcttacc ttcttctcct
    14461 tgagttttca tgatataatt taaaacgccc agtgtcgcac gtctaacagt ataaatattg
    14521 attctattag ttcccgctcc tattatacct cttaaaccac ctgttccaaa ctctaaattt
    14581 ttataaaatc tatcttgtat ttctttttca tcatctttta tacttaatag ctcatttttc
    14641 gtttgttcat caaaataagg agaatttaac cacatctcat aattattctt ataatccata
    14701 ttttcatcct ttctgactct aatttaaaat ttcaaggtaa ttatctatct tctaagtagt
    14761 ttttgttttc cttttcttaa aaattcaaaa gctaaatcca tctcttctac cttaaataaa
    14821 actatcaata cagtatatac agaagctcca cctaatactc cagctccaac tgatataatt
    14881 tctttaattg ttcctgaacc cataaatgca tatagattat tatatatcaa taaagtagct
    14941 actgccatca caccagatgc taccaaactt tttagaccag tctttataat tttatcccct
    15001 ccaaaatatc catttttctt tttaagagat ataaataata atataactgt aattatgttt
    15061 gatgtactag ttgatatagc aatacctgca tgacccaatg gtcttatcaa tattaaatta
    15121 agaactatat ttattattaa agctatactt ccatttatca ttggcgtttt tgtatctgaa
    15181 agagagtaaa atgccctata taagacatct cttactccac aagccaataa acctagtgaa
    15241 tataatctaa gcgctataga tgtaaggtct gttgataacg catcaaattt acccctttgg
    15301 aataatattc taacaagagg ctctgctaat ataatagctc ctatagatat aggaagcacc
    15361 accaatataa tacaatttga agattttaca atagtactta taaatccttc tttattatct
    15421 ttaccagaca tttttgctag tttaggatat ataactgtta ttatagatgt aacaaataat
    15481 gccataacaa actcatttag cctatttgca tagttaagag cagacaactt tccatcaccc
    15541 aaagtggttg ccaataatcg gtcaacaaat atatttaact gtccaacagc tactcctacc
    15601 aacataggca atactaggtt gactaactct tttatggact catcctttaa atttatatat
    15661 ggcttatact tgtaggactt cttaaacgca aatggcagtt gaaacaatag ctgacttgcc
    15721 atagccaata gtgctcctgc tggcagtatc catacatttt tacctgcact tagtgctatt
    15781 gctgctatta ttataatatt atatgggatt cctataagcc caggaattac aaaactgtca
    15841 ttcacctgta aaaacgaact aaatatctta cttccactca ggaataaaac tcctgaaatc
    15901 attattttag taaataatat tgctgcttgg aacttagcag ggtcgtttct aaatcctgct
    15961 gcaaatactg taacaaattg ctctgtaaat aaaagaccaa taatagctat tactatagcc
    16021 attatatagc atatatttaa aacattatta gtaaatttta aggcaccttc atctccgagt
    16081 cttttctttg tctcaaagta cataggtata taagttgtag caactgcaga gcctatgact
    16141 gctatgatta aaccaggtat atttaatgtt aatacaaata cgtctgcata catacctgtc
    16201 ccatagaaat ttgcaagtac aagttctcta aggaaaccta atacttttga aaacatagtg
    16261 gctgccataa tccacaatgc tgctttagct gtttttgaca tcttctttct ccttatctaa
    16321 tttaaactct aaaagtagaa cttttaaaac tgcttataaa cattattcta attaagtaaa
    16381 tttaacctat gaaaagcttc cttataaatc aaaaactatt tcgactcata tctaagtgcc
    16441 ctaaatgtaa tcaatagtaa taatagtatt aatactatac aagataagaa tgttagatta
    16501 aatatgctct taaatagact ataatcacct actgtatacc caacttcaac tacatcattt
    16561 atattgctat ctttcaagaa tttatcttgt gcacgactta agttatcact acaaatcagt
    16621 attggcatat tttcttttgc tgatactact cccaaagaaa tatattcgtt tacatcagat
    16681 ctactaccaa agtctaatct agcagtatag attttcttta tatctttttc atcataaaac
    16741 ttctttatta aatcaacatt tgtacttgac ttatttattt gctttattcc ttctatattt
    16801 tttaagttag aatcaatctc tgcaataaac tcttttgaat cacctaatgc atatattttt
    16861 ttaagatttt tgccttttaa gaaatcaatt tgttcctcaa acccttcatc tttagcccat
    16921 attattggca ttccactttt agcggcataa gaatatacag atatggcgtt ctctaatcct
    16981 gttgttgagc taagtaaaaa tgcttcatca aatcctactg ttctatacat ttctctagct
    17041 atctttaaag atgtatcata tctgtctttc cctcttattc tttctacttt cattcccatt
    17101 ttttctatat tacgttcagc atctttagat actgccttta aaccacctac tatcgtaacc
    17161 ttctctggac ctagttcttt tagataattt cttgttactc taccaagatt tttactctta
    17221 gttacaacta ttggagcatt ttttgcatat gctaaaggtg ttgcacttat actatctaca
    17281 attgactctt catttacaag aattacttct ttagaatcct taaatgctct tttatttaat
    17341 tctgtcgaaa tctctggtat actcccttct atcaattctc ttttgggatt tgcatctgca
    17401 aatactccta tcgtactaga aatagagatt aacaaaacta acgataatat agtagaaatt
    17461 tttttaatca taagttctca ttccttttct tttttgtatt ttttgacaat aaaaaagctc
    17521 tttttaaaga gctttttcat tgattagtaa tctaatattt taaaattatc tagctccgtc
    17581 tcctgtcaat actactttaa cagttttaaa gagtatctta aagtcaagta atatactcct
    17641 attttttata tagtaaatat cttctttaag tttttcttca ggagtgattt cgtatccacc
    17701 atttacttgt gcccaacctg taagcccagg ttttatagcg agcctgttga taaatccagg
    17761 tatttcctca ttaaattgaa cagtaaagtt cggtctctct ggtctaggac ctattaagcc
    17821 catgtccccc tttaatatat taaacagctg tggaatctca tcaattctag ttttcctaat
    17881 aaactttcca accttagtta ttctaggatc atctttttca gcccattgtg cgccaaattt
    17941 ttctgcgtct gttctcatag atctcaattt atatatatag aatcttctcc cgcattttcc
    18001 tagtctttct tgcttataag ttattggtcc tttgtcctct attttaataa gaattccaaa
    18061 tattgctatt aaaggtaaac ctatcacaag tcctattata gataaaacta aatccataat
    18121 tctttggtac aagtcgaata aaactgttcc ttttactatg ctatagtcaa tctcacttgc
    18181 aatttcttta tctgcccctg gatagctttt aattttaggt atttcacact catcattagt
    18241 atatccagac atagttacct ccatattgga ataaaaattt ttttaattct taatttcaac
    18301 ttatttctat cttatttttg acttatttct atattatact caataatgag cagattatca
    18361 atacaagaaa catcattgta aacaatttga ttctttacaa aaatatatca tctcttgtta
    18421 tattttacat tataacagga gatgatatgt tttgctttaa tattttctat tttattttac
    18481 tgtatcttct attcctattc caatttgagt tcccattgtt tttacttttt ctgcagtatt
    18541 taacttatcc atatcgctct tattgtcaat aaatgcacat tcaacaagta ttgctggata
    18601 attattattt cttaacacat ataggtaatc ttttccatta tcaagtgttc ttgtcttgat
    18661 tcctctattt ttcatattaa atttttctag tattcttttt aagatatttg atgctgcagt
    18721 cttagttgtt ccaccatttt tatcttttac tttgtaataa atttccacac cattaccaga
    18781 tccattagac gcattgtaat gtatacttgt aaataaatct ggctttatag tatttgataa
    18841 tgctgttctt tctcctaaag ccattgtttt atctgtatct ctagtcataa caacatttat
    18901 accttttgaa cgtaaatatt cagtagttgc taatgctgta ttaagagtat atttcttttc
    18961 ttgtgctcca ccatttaatc cacttgttgc ccctgaatca gaacccccat gacctgggtc
    19021 tatcactaca gtttttcctg ctgctgaacc tgaattatct ggttctgttg gtgcattgtg
    19081 ttttgataaa ctagatgcta tagaatttat aacactatct ttcattccat caccaatttg
    19141 atatactgtt tctgctgttt tttctgataa gtttgtacta tggtatgcag aaacataagt
    19201 ttttggagtt ataagtattg gagcttttaa tttagctgct aaaggtcctg ctgttattga
    19261 atcaactatt atgtcagatt ttgcaactaa cattgcactc aactctttat ctggatagaa
    19321 agtcttaatt acatttgcat ttgtttcatg tctatctgct ccagaaactc tattttttga
    19381 agtaccattt tttgctattt tagatatttg gtctataatt tttgatgata aactttgact
    19441 accacctatg taataagctt cttctaaacc ttgtgaggat aaccaattat atgtaccttg
    19501 tggtacagaa tctttattag ctaatattat aggttgttgg tcttcaccag cttttgatga
    19561 aatattaaga gcatcatatt caccagcata tccatttgct atgtatattt tattaacatc
    19621 atgatactta tctatttctt ttgctataag taaagatgtt tcatgtctat caactccacc
    19681 aatacgagtc acatttacat ttacagcaga tttaatttga gacactgcct tttgtgaaac
    19741 tgaaccatca tcacctataa ttattacatc acttggattt agacgtttta actctgcttt
    19801 tgtactttct ggtatatcat ctttatttgc aagtaatatt ggtgcgtcat atgtagatgc
    19861 aagtggagta gctgtaattc catctgctgc taattctcca ttaactatta caaccttact
    19921 agacccatct ttccaacctt gtttgcttat ctcaacagca gtttcaaatc tagtagctcc
    19981 agcaagtttt tttactggtg cagaagtttt tctcatatat tcatttactg cttttgttga
    20041 atttgcatca cttattataa actcttgtgt aacatcataa tccttaagat ttaatcccat
    20101 agactctaag tcttctttgt ttaaaacttt ctgttcatct ttaccattta tttttatgct
    20161 ttgaagttcc catacattta agtattctgg gtctttgtta tagtttatta caccttcata
    20221 tgaatctgtc ttttcattgt aatttaaagt agcttcaagt ggcatatcat aacacataaa
    20281 atttaatgtt attgaatcag catttaattt ttctttaaat tttacacttg cattagattt
    20341 acttcctaac actacacttt ttctttgtaa atttatatta atatctgaat tagattctgt
    20401 atttgcagta gtattttcag ccgctaaagc tacatttggt gccaccattg ctacagacaa
    20461 cattcctgtt gccaataatt ttgttgtttt tttcatcata tcccccctaa ttttttggtt
    20521 ctttaaatct ttaaatcttt tttaatacat cttaagcttg ttttatatta cataaattca
    20581 atattatgta aatctttttt cattatagca caaaaaatta caaattgtaa actttttgta
    20641 atcttttatg gcatggtttt taaattttat cgaggagaaa ttttgattat atttgatagt
    20701 ttccgtatat ttgaacaaat caaagacttt atcatctaaa aatattgcac taaattttaa
    20761 taaatatttt tttcttgtac attatagaca ttactataac ccagaatagg acatacaata
    20821 aagaaaatat aagagaatcc caagttgtac ctgcccaagg tgttataagt tcatatgtag
    20881 tccactctac caaggtagtt ggataatcga ctttgttcgt aagttttgga acattccaaa
    20941 aaacatgact caatatttca aggcacatat atgtaaaaat agggctactt ccaagtgcta
    21001 ttattggggt aaatatttta cttttatttt tgatgtcgca tataaaataa aatattgaca
    21061 gtaatatacc ataactgcca gccattaaaa gtacaaatga gctagaccat aatcttttat
    21121 taaatggaaa atactgatta aagataaaag cacctattag caggattatg ctcataacca
    21181 gtattttaaa gaatttttta tattctccta tgttttcttt taaaagtaca catcccatag
    21241 tgcatcccaa catacctgaa gaaattgcta ctatactagt taaaaaacca tctggttcaa
    21301 attccggctt gtacaaatgt cccttgaaga aatgtaaatc cactaactgt gcaagagaac
    21361 cttctagctc aaatccataa ggttttgcta atatgtagta cccaactatt ataaatgtag
    21421 atatacatag aaaagtaatt attgtagcag tacttccaac atttaatttt tttagtagta
    21481 aatatactaa actagtcaca aaatagacta atcccatacg ttgaagtact ccaagtattc
    21541 ttacagtatc taaatcagga tttcctaggt aatttaagaa aaatccaaat aatatcaata
    21601 agatacttct cttgaatata ctaagtatta tactcagtgt ggatttatta ttttttagtt
    21661 tactatttat tgatattgga attgtaacac ctaaagatat aacaaaaaaa ggaaatgcaa
    21721 aatctgcaag tgttacacca tgccatacag catgtcttaa ttgaggatac attctcatcc
    21781 aagtcccagg attattgcag actatcatga gggctattga tagccctctt attatatcta
    21841 ttgatttaat cctactattc ataagtttag aatctacagc attatttgaa attctattgc
    21901 tattgctatt gctattgcta ttgctattgc tattgctatt gctattgcta ttgctattta
    21961 gattattttt ctttattcta gttttgtcaa tacttttatt tatttttttg ttacattcaa
    22021 ttccaatccc catgttacct ccccaaagct tattcaaata tatactaaac tacatattta
    22081 tctataaatc tatttgtata taaacctgtt taactacata actttagaac aattacaaca
    22141 aacacttcac ttaaatatat atattcttag ctaagaaatt tctttagaaa tccaagctta
    22201 tctagtatat cttgtatttt attgactttt tgacttatag aatctatagc tttttcaatt
    22261 gaatctactt tagcctctaa atcttcaacc tgattaactt ttttatctat agtaccatta
    22321 ttagttatat cactattaga tgaattatct ataagttttc ctatatatcc atcattaatc
    22381 aattttgtat cattagcaga agctaatatc atcaacgtat ctatatctga atttctagta
    22441 ttttctataa ctctaccatt tttgtcacga actgacaata tagtgattgt atcatggtta
    22501 ttaaatccac cttctccaat atttctaact ataacattta ccaaagtagc atagttattc
    22561 acttcaccat taggcatatc tactgtaaga gtctttgaac aatcaccctt tatattcaca
    22621 gttattggtt tatttgttac cattctaaaa gcttcattta cagtattctc ttttggttta
    22681 aagtttataa tatcattagg acttgctata tttatcatat cagttaattc ctcaacagtt
    22741 tttgcttcaa acacagtttt ttcttgtaga cttttaattt catcaaaagc ttcttcattt
    22801 ccatttccac ctacctgtgt tatagtattt aattttttag tactaagtat gtccctttgt
    22861 tttgtgttta atttattacc aaccaaaaca acaggagact cattttttgc agccaatact
    22921 ccaacagcta acgagtctat tagttgattc tcatttttac taccatcctt agttacatat
    22981 aaattactta aatttgtatc agtgtaaaac ttttctatga ccttagcatt agtctcattt
    23041 ctatctttcc cactcactct ttctgcatta ggaagactct tttctacagc tcttgaaaca
    23101 gaatttgtac ctccaattac atatgctttt ataactttct catcccttat aaatttatca
    23161 aatgcttcta ctccgtcctt aggactagac aaaattatag gcatcttatt ttgagctgct
    23221 ggtgccccaa cgctaacagc atcactcaat cctttttcac catttactac agcaacttcc
    23281 ttaatatctt ttatatcctt aagcttatta gccaaaatca gtgaagtttc atatctatcc
    23341 ttaccattaa ttctttctac attaagtcca ttacttttta acttgttctc tatatcttta
    23401 ttaagtacag cagtacctcc tatcaaataa atatctttag ctcctagacg ttttatctct
    23461 ttttcagtcc tatcatctag cttatcactt tctgtcaaaa gtattggtgc tcctttagcc
    23521 tttgcaaatg gtgtagctga aagtgcatca gctaatgaat tgtcattaac tagcactaca
    23581 ttaggactag aactgtatgc cttttgactt atctttacag cagtttcata tctaccatta
    23641 cctattaatt tttcctctgt aagtgcatat gatggtatta tagaaatgct acttaaagta
    23701 agcgctattg tcagtattgt taaaatagtt tttggtgctt tcattattat tcccccttat
    23761 tttgtttaaa atcttactat atttatatta taaaataact ttacactatt ttacagttta
    23821 aaattattac atttttcatc aataataaga aaactatcta aaaatataga caatcctatt
    23881 tttcattatt taaatgataa aaatataaaa caacaaaaaa gagtgcctca tgaactaaaa
    23941 agttcatttt gagacacccc gttttgttat tttatattta taaacttcta ttgaatattt
    24001 tttaataata aaatgtatat ccttactatc tttccattca ctattttcct aaaagagtat
    24061 ttagttcatt aaaagcattt tcatttccat ttccaccaac tctaatagga gtttctattt
    24121 tcttagactt aagtacattt ttttgacttt catctaactt atttccaact aaaactactg
    24181 gtgaatcagt tttagcacct aatgctccta ctgctagtcc atctatcaag tctccttgac
    24241 tctttgaacc atctttaaca acaaaagcat atttcaaatc tgagctagga tagaatttat
    24301 ctattatctt agcattagtt tcatttctat tacttcctgc aagtcttgtt ggatttgaaa
    24361 gcttactttc taaatttgat gataaagtag ctgttccacc aattatataa gacttagtta
    24421 ttttagatga atttaaaaac tcatcagcac cttgcaactc actcttttca ttagtaagta
    24481 ttattggcat attgttatca gcagctgctg caccaacact tattgcatct gcaagtccat
    24541 ttacaccatt tgccactgca acttgtgaaa cagattttat actatttaac ttttgagcta
    24601 gagatatact ggtcttgtat ctatcactac ctgaaattct ttctactgat atacccatat
    24661 tactaagttg agtttgtaca ttcttgctta aagaattttc tccacctatt agataaactt
    24721 ttttagttcc taatctttgt aactctgcct tagtttttga atctaagtta tcttttcctg
    24781 tcaaaagaat tggagagtca attgctgctg taaatggtgt agctgttaaa gcatccacta
    24841 ttgcatctcc atttactaat actgcatttt gtgaagaagt ccatcctttt tgactaactt
    24901 taactgctgt ttcatatcta tctgctccag ttaaagattc tcctgaagta gcttgtccag
    24961 aagatgtttt tgttatagct tttataacaa agttacaagg agaaaattca ctatatgtat
    25021 ttatatcttc aaatttattg ttttgaagta tgtagctttc acctaagttt gctttagcct
    25081 catatagata gtatccatct atatcagttt catatgctaa agttgatttt tctctattag
    25141 gatttgctgt gttgtctata actactacta ttgctccttt accttttggt aagctgtaag
    25201 aattagtagg tacatttatg tatccagaat atgatactgt tccacttgca agttttgtca
    25261 ttgaattgtt ttgaggaact ccatttacta ctggtgcata atatacttca tattttgctc
    25321 ctacagaatc tgtttcaaac ataacagagt caagtttttc agaatctctg ctaaaatcaa
    25381 atacatttgc tgctgttact ttatttgaca ttatcttgct aagaccagca tattcaagtt
    25441 ggtacatttt tttatcgcta tctggttttg atactgattt cattgcgtag ttatctgtat
    25501 cttttaaaag agttttgtct tcataagaaa tccagaaaaa gcctttttct gagttaaatt
    25561 ctccccaact actttttact aaccatgctc catttgattc tggtctaaca cttggtttga
    25621 acttatcttt tgaataatta tcatcccatc ctacaattga aacagaatga tttaaaggtt
    25681 cacttttatc attacaatta tacgaatttt catcatcact taagtaggct gaatagtgtg
    25741 catatcctga tgtcactgca ccatattgca ttattgcatt tttaacagaa gttttatctt
    25801 tactaattct tacaacatct gttacattaa attgtgtagg tgcagtatcc atattttgag
    25861 gcctagttgc tccttcatct tctgatttaa aattatatgg aatgtctttt tccagcttag
    25921 gtcctgccca ggctgtaaga tatccaattg cagttacatt cgaactacct gtcatatcag
    25981 ttaagttcca tccatgtatt cctccagttg accaccatct aaaatgctct tctgaaagat
    26041 cataagttcc ataacctttt aatttcaaat atgcttctag tgttgacatt ccagaaaaag
    26101 cccaacatgt attaagtttt ccttggtctt ttgctggtgt agttaaatct aattttcttg
    26161 gatcatatga tacacttgat ttatatgatg ctggaagact acttcttaca ttagttttaa
    26221 gtgttgttcc ttccatttca taaggagttg gtattactcc attatatttt gctatatctc
    26281 cattttttac atcttccaag tattgaagat atgattcaga atactctgca gtttctactc
    26341 cagtcagaat tttatttttt tctgctgaga caggtattgt tgacacaatt aaaaaacaaa
    26401 ctgtcaacaa caccagtagc tttgacaatt tttttaattt atactttctc atatttaccc
    26461 ccttgtatgt atttaatttt tattatatca gatttctaaa tctaggtcat attttatttt
    26521 tataaaaata ggaattataa ttatacatat ataaaatact attttatact gcatatttat
    26581 tgattaatat gtggtatact aattacataa taatatatac caaataatgg aggaaaattt
    26641 tttgatatta aaaaaacatc tagaaacaat attatatctt ttttttggtg ctttaacagt
    26701 tttaataaac attattactt acctattttt tacaagaatt attaatttta cttttttatt
    26761 atcaaactct ctatcttggt ctatagcagt tttattttca tatataacaa acaaattttt
    26821 tgtattcaaa agtgaaaaaa ctgagttttt atttctatta aaagaatttg tatctttttt
    26881 atcttgtcgt ttattttctg gttttataga aatattactt atgtatttaa tgattgattt
    26941 attttcttta aatgatatct tagtaaaaat aggcactaat ataattgtga taattttaaa
    27001 cttcgtcttc agcaaggtag tagtttttaa gcatagaaat taacattatt atatttatct
    27061 ttatgcagaa taaggagttg cctaaaaaaa acaactcctt attagcttta agaaacaatt
    27121 tctattttat ctgttgctcc ccatttttca actttattat tttctcctga tgctaattct
    27181 acataaagtc catttacctt atttccttct aaaattatat cactattaga agtacttttc
    27241 caactggagt ctatttcatc tcctacattc atagcttctg gttttgtagt ctttattcta
    27301 tagtaaactt tattaccatt gtatccagtt acactaatag ttactttaga attttcatca
    27361 acagaaacag ttgattttat tccatttgct actattttaa aaccatcatc tgtcgcacca
    27421 gctcttcccc atcttgaaac tttctttctt gtataatcaa tttcaacaac ttctatatat
    27481 gatccattct tagcatttac ttttaatggt tccgtatctt caaccaagtc ccaatcattt
    27541 atgttaattc tatcaccaac cctcatagct gatggtttat atgacaaaac tctataataa
    27601 attttataat ttgaattttt aggtttctga ggtacagtta acataatttg attattctca
    27661 agaggagaac atgcacttat ctttggagat aatcctgctg ctgtatcact atcatcatct
    27721 ttatctgtat tattagtgtt aggtttattg ccacttattc ctggttcttt atttccacta
    27781 acagtatcaa tctttcccga atttcttata ttagtacttg aagaatcatt atctatcttt
    27841 gttatagttc catcatttct tatatttaca tcttttgcag aactaaatat tgttacaatc
    27901 catatttgtc cattattttc attttcaatc ttgcaaccat tatcatcaga aatgtccatt
    27961 tgatttatat atcctgaatt agttaaagtt ccatctttta tatctttaag tatcaatgtt
    28021 ttacttatat cacctgtatt attcaactct ccatttggca tatttatagt tattgactga
    28081 ctatattctc catataaatc aacctttata gctttttcag tctctaattt aaaagattca
    28141 cttatttttt tatcaggtct aaactttata atatcattag catccacttt ctttaaagca
    28201 gaatccaatt cttcaattgt ttcaactgta tatttagttt cttcttgcat atccaatata
    28261 tctttataga cattttcatt tcctattcca ccaacctgag taactgtact aaatatctta
    28321 gtatttagta catctttctg aatagaatca attttatttc ctactaatac cactggagaa
    28381 ttgttttttg cagccaacac tccaactgat agtgaatcta ttaaatcact ttgttttttc
    28441 attccatcct tggttatata tgcatttctt aaatctgttt ctttataaaa ttcttttata
    28501 acattagcat ttgtttcatt tctattattt cctgatatcc tcttagaatt tggtaaattt
    28561 ttttctattt cattagaaat ggaatttaat cctccaataa tataagaatt ttttatattt
    28621 tgggctttta taaatttatt agctacttca actccaaggt ttgaatctga aagtataatt
    28681 ggcatgtaat tttgagctgc tattgaacca acacttacag catctgctaa tcccttttct
    28741 ccatttacta caattatatc agaaatatct ttttcattat tcaatttttc agccaatttt
    28801 aaggatgttt catatctatc attcccaaaa attctttcaa ctgttatttt ctctgatttt
    28861 aattcatttt ctacatttac acctagtgca tttgaacctc ctactaaaaa tacatttttt
    28921 actccaagcc tttttagttc tgattttgtc ctaaaatcca attctttact tccagatagt
    28981 aaaataggtg catctttcaa tttagcaaat ggagttgcag ataatgcatc tgctattgag
    29041 ttatcattca ctaatacagc attatcagat cttaaccaac cgttttggct tatttttatt
    29101 gctgtttccc atctacctga acctattaaa ctgtctttat tagatgcatt taccacatta
    29161 ctaggtaaaa caattgataa aaatgctgct gttaataaag atattatttt ttttgatatt
    29221 tgcattaatt atctcctcct aatttaaata taattttatg gagcatgtac atactgtgtt
    29281 ttatttaatt ctaaatcatc aaaatgagat tttactgaat ggtatcctat agcataatgt
    29341 ccactttcag gattccattt cttatatgca ttcatcgcac ttaataactt ctcctcttga
    29401 ctaggtaata ccttccagac ttctacattt ggattaactt ttttaaaatc cttatagtca
    29461 tatggttcta cataaaatct taaatctttt atttttcctt ctctatataa ctctagtgca
    29521 gcttctccta atgctctatg atcttcatgt attccactag ctttaaacgg agtaacagtt
    29581 tttacttttg cttctggata ttcttctaag tatttaagta ttatttcctt tgccttttct
    29641 ttgcttaact caccatcatg agctctatta tcttctatat gtatattact ttcttttaca
    29701 cccattgctt cacagctatc cttaaattct tcatctctat cttttgaaaa ttcactttct
    29761 gataaagagt atttatgaac atctttaata tgataagaac atgaccctcc atcatccaaa
    29821 acttttctta ttatagattt actaccatct gtgcacaata tgacatgtgt atcacttttt
    29881 tcaacatgtt caattatagc acttcccata cttaaggttt catcatcttg atgtggtgta
    29941 aaaaacaaag ttgcatctac cactactaca tcctcctact ttataaaatg ttataattta
    30001 caataaatag cataattata gaaaaagatt cctgtattaa ttaaggaatc ttctccacag
    30061 tataagttat taccaaccta gtattttagc tatcttttgt attactgtca attcaactcc
    30121 attacctatt tgagttactt ctgcaccaag atttaatttt ttactaagtg cattcttttg
    30181 tgagtctgtt atatcagttt tagctagaag tattggacct ttacctgctg ctagaggtcc
    30241 tgctgccaat gcatctacta gcataccatt atttccatat ccatcttttg ctatatatag
    30301 tttatctaat ttgccataat atgtttctat aactttagca ttagtatcgt gtcttgtttc
    30361 tccttcaact ctttgaactt ttctatcttt atcaatatct gcaagttgat tttcaatttg
    30421 gctagataca ttattagagc caccaactat gccaatctct tttccatcca tcaatttgat
    30481 agcatctgct gatagatcat tccaaccatt tactattata ggagccttaa gttcagcagc
    30541 tttagcagct atactcatag catcagcctc cccttttgcg cctactacaa atgcatcttt
    30601 aaaagacccc atagcttttg ctactgctac agaagtcata tgtctatcat ctccagctag
    30661 tctttcaaca ttcttagtta ctgattctaa ttgcttttta gctgtatttg atactgctga
    30721 ttctccacct actatatata ttttagctga tgggcttttc ttaattatat cttttatata
    30781 atctaaagtt acttttggta tttcattatc agatgctaat aatataggtg cttttttaga
    30841 ttgagcaagt ggtgtagctg ctaatccgtc aactagttta tttgaattaa ctataactat
    30901 attgtcagta tattttgttt gttttgctat agccactgca gttgcatatc tatcatctcc
    30961 agctatcaat ttaacctttg aattaccttc taatgcagct ttggcatctt ttaaatcttt
    31021 ttcattggta ctttctacag taagctctgt tgcttttcca gatgcatcag tatacttaat
    31081 agtaaattta tattttcctg tttctgaatc ttttgctgaa atatcactaa ttgactttga
    31141 tgtaccattt gctaccatat tacctttaac agtcaatcta tatccatcat ataaatcttg
    31201 tggtgaaact gtatttaggt cactattttt tatagttatt tcggccaatt ggacatcatc
    31261 aatattttta gctcctgcta cacctagttt atcgatttta tttactctaa atccttctgc
    31321 agaagggtct aaatttgttc cagttgaatc aactggattt gctgttaaat caactttttc
    31381 atcaccaata cctacatata tagttttaga agttactgtt tctgttttat ctcttaaagt
    31441 tataactaac ttatctttag cactatcata tgaagctttt acatctgcaa ctttataaat
    31501 tccattggtt tcagttttag ctttttctgt tatagaatta gcctcagcgg aaaggtctgc
    31561 tgaattttca tatttttcag ttgcttctgc aactacttca tttgcactat ttgttatatg
    31621 tcctttatct gttatagtta caataagttc tttattttct cccaaagcat ttactaattt
    31681 ctctaattca tctatattag ttattatttt taattttgat aaagtctcac caacttttat
    31741 ttcatatatt ggttgtccaa cttgagaacc tcctgtatac ttttgagaca ttaaacctct
    31801 aactttcgaa actaattctg tagcctcttt cttagttatt gtttcttttt ttacctgtgt
    31861 agtttctgca gcaaaaactg gagctactga tgtacttatc attgcagcag ccataattac
    31921 agaaagattt tttttattca tggtattttc cccctaaaaa attcaaattt ttattatttt
    31981 ttccttattt accaattata atatatattt gatattattt caataaatga gacttattgt
    32041 aataattaac caaaagatat aaactttttc ttatatttta tttttttaat atttacgtat
    32101 acaaaaaagt aacaaaattg taattttttt taataatcca gtttataact aatagacttt
    32161 tcatcttctt tatagtataa tattaaatgg aagaaagaaa tttcaagcat ctcttttttc
    32221 cgtatttctc acatagaaaa agaggctgat tatcagcctc tttttcctat acagtttaaa
    32281 tttcttaatt aaatttatat aaatactgta tagtcgtctc tcttatattt ttatttaatg
    32341 attcaaacat ctcataacct tccatagcat attctttgaa aggatctttt tgagcatatg
    32401 atttaaatcc tacatattgt tttaattgct ccatagcatc tatatgttct acccaatatt
    32461 gatcaactac ttttaaaaga acaatttttt ccatttcggt tattttgtca aggcctacta
    32521 ccatcttttt taaatcataa actctttttg atatttcata agtactatca attatatcct
    32581 caatactttt attatctaag tcaggtatta gtaaggtatc agctggcata aatgtattat
    32641 aaagatgctt aaaatatcca tagtaatccc tctttctttt tgttaaatat tcagcagcag
    32701 cttcttttat tatatcttta accatgtttt gaatatctac ttgtatatct tcatcattta
    32761 gaactttatt tctttcgtta tagattactt ttctttgttc atttatagta tcatcatatt
    32821 taagaacatt tttccttaat tcaaaatttt taccttccac ccctttttga gctctctcta
    32881 ttgcctttgt aagagattta ctttcaatag aattattttt atttgaattt ggtcgtttca
    32941 ttagtttctc tatacttttt ccaccataca atttgattac atcatcttca agactaacaa
    33001 aaaaccttga tgttcctgga tcaccttgac gtccagaacg ccctctaagc tgattatcga
    33061 ttcttctaga ttcatgtctc tcagttccta ttacataaag tcctcctaat tcctttactc
    33121 tactttcttc ttctttatca cctgctccta aagcaatatc tgttcctcta cctgccatat
    33181 tagttgcaat agttactgaa cccaacttac cagcctttga cactatcaat gcttctttat
    33241 cattttcctt agcatttaga acttgatgtt ttattccttc tttcttaagc ataccagata
    33301 acatttctga tttctcaaca gaaacagtac caataagaat tggctgtttt gttttatgta
    33361 tccttattac ttcttctaca acagcttgat acttttcttt ctctgttcta aacactttat
    33421 catgaagatc tgctcttata attggtttgt tagtaggaat ttgtacaaca tttaatttat
    33481 aaattgattc aaattctcct tcttcagttt tagcagtccc tgtcatacca gaaagtttat
    33541 tatataatct aaagaaattc tggaatgtaa ttgtagccat agttttagat tcatctttta
    33601 tttctacccc ttcttttgct tctatagctt ggtgtaagcc atctgtatat cttcttccat
    33661 ccattactct tcctgtaaat tcatcaacta tcattacttc ttgattaaca acaacataat
    33721 caacatcttt ttccataagt ttgtgacccc taagagcctg atttatatga tgatataatt
    33781 ctatattttc tatatctgta agatttttaa tcccaaaaaa tgattctgcc ttatcaatac
    33841 cactttcctt tagagctacg gtcttatctt ttctatctaa ttcataatct tcttctttta
    33901 ttgtttttat aaatgaattt gctaattcat aaagttttga atcttcatca ccctctccag
    33961 caattataag tggagttcta gcttcatcta taagaattga gtcaacctca tcaactatgg
    34021 caaaattcaa ctctctttgt actttatcaa actttttagt taccatatta tcttttaaat
    34081 aatcaaatcc aaattcatta tttgtaccat aagtaatatc acaattgtat tgaacttttc
    34141 taacttcagg tataagatct gcaataatta ctcctacaga catacctaaa aactcataca
    34201 ctggttgcat tagctcttta tcacgttgtg ctagatagtc atttactgta acaacatgca
    34261 cacctttacc tgttaaagca tttagataaa caggtgcaac ttctaccaaa gttttacctt
    34321 caccagtttt catttctgct atttttcctt gatgaattac aatgcctcca attaattgaa
    34381 ccctatattg acgcattcct aaaactcttt ttgaagcttc tcttgccact gcaaatgctt
    34441 ctggtaataa actatctaaa gtttctcctt tttttaatct atctctaaat aggtctgtca
    34501 ttttttttaa ttcgtcatca gacatatttt ctatatcttt ttctaaagat tctattttat
    34561 caactatcac attaagcctt cttatttcct gctcatctgc tttatccaat attgtatcca
    34621 aaactgacat atttaattta cctccttttt tatcatacaa ttactattat accttaaaat
    34681 tatagaatag tagacaatta tttttattta tagtataata atcatactta aataaattac
    34741 atctttaatt atttaggtat ttctcacaaa aaaaggaact atttttagtt ccttttttaa
    34801 tttcttttta taaaaaagag aacttcttca aagaagttcc ctcttattta tagtttaatt
    34861 ttatcttata attctaataa atcttttatt ctcttaataa ctgaatctgc tattccttta
    34921 ccaacttgag tcaatttctt agaatcatca gaatttgtaa ctttacttat tgctactgat
    34981 tggtcagaag ataaactatc agttgctaaa actattggag cttcattgtg acctgctact
    35041 gctgctattg ctaacgcatc tactaattga tcttcttttg tagaaccatc tttagcaaca
    35101 tagaagtttt taacacctgt gctaattaca ctgtcactat tatctttttc aaaataagtt
    35161 tttataactt ctgcattagt atcttgtcta tcatctccac taactctttc tggactctta
    35221 cctgtagcat catctatagc atcttccatg tcttcagaaa cactagattt tccacctatt
    35281 atatctacat aagcacttcc tataaagtct tttgcatcag ttgataaatc ttttcctttt
    35341 ccatcaacta ctactattgg catttcttta ttagaagcaa ctgatgctat actcattgca
    35401 tctgctaatc cagtaccacc aactacaaat actttattat ggtttaaacc tatttcatca
    35461 gcaatttcta aagatgtagc atatctatca tcaccagata atctttcaac ttttaatccc
    35521 atatctttta attcatttgc tacttcttta gatacagaat tttccccacc agctatataa
    35581 acagtctttt tagatgttat accagtttta tcatctaatc ccattactct ctttatttca
    35641 gattttactg atgaatctaa cttatctttt gaagttaata ataatggagc atctttaaca
    35701 gcagctaaag gtgaagctac tagaccatct actattgatt gtgatcctac taatactacc
    35761 tcattagctt ctacataatc tcctttaatt cctccatctg cattataaga gtctttactt
    35821 atttctattg cagtctctat tctatcatca ccagctaata cagaattatt tgaataagaa
    35881 ttattataag ttcttaattc atctaaagct gattttaagt tattcttatt agtagatctt
    35941 aatgttatta tagcaggagt atctactcta tcacttgtag gtaattctgg cttatagtta
    36001 gatgatgcat atgttgaagc tgtatttaat cttttacctg ttggataaag aactacctca
    36061 taataatcat tgtcagtttt ttcaaaatca cttgctgtta ctgtcttata tgcatcttct
    36121 aattcagtat ctttaaatac atatttttta gctaaagctt ctgctttttc ttttgcatct
    36181 ccagatattg taactgtttc atctgatgct gctataactg ttacatttga ttcaactgct
    36241 ccaacttttt ctccagtttt ttcaaaactt aattttgatg taccatcagt agtattaacc
    36301 gttataactg gtttactaaa atcatatatc tctttagtac catcaagaga tgctactgct
    36361 gtatctaaag taggtgctac tgctgcttgt gtatcatatt ttaatttaat tgcaccagct
    36421 actgcatcag catatgttgc tgtaatagct actgttcctg tagcattatt accagtctta
    36481 tcagtaaatt ttgaagtttt gactgctgcc tttgtagctg ctaaaacttc atctttagta
    36541 gctccatcta cataagctga cgtagttgta tcatattttt gtaactttaa tttagcatca
    36601 ttttctattg aatttccatc ttgatctaca atcttaactg ttgttgcagt accaacttta
    36661 gtaactacag cattactatc aagttccttt aataatgtat catcatattt tttttctgtt
    36721 ccatctgtaa tttttacaga tatttttgca tattctccag cttttaaatc tttaatttca
    36781 tctttaacat aacttatttg acttgcttct aaacttgata tagatacccc ttttggattt
    36841 ccttttttgt caaaaaagtt aactgttatg cctgttacac catctgcaac atatttttta
    36901 agtattcttt ctatatcact atacttacta ctagatacag tggtatttcc atttggaaaa
    36961 ctatcctctg ctgcagcgaa aactggtgct gctgatccga ttactgtaac ggcagacatt
    37021 acaactgcca aatttttctt attcattcct taaattcctc ccaacattat attaattaca
    37081 tctctttaat tatgtacatt aaaattccat gttcatataa accaaatatt tctcacactc
    37141 ttatgataga ctatataaaa tagaatttca ataatttatg caaaattaat agaattttct
    37201 ttcaacattt ttcccctttt ttttgctatt tcatattttt taaattaaat tttcaatttt
    37261 aattttatta atacatttat ttatctgatt taatcaatat atattaatat tttctaccat
    37321 aaaatattaa tatatactct aactatttaa aaattccatt gtagcattaa cttctctttg
    37381 attagaaaca tacattccaa agccaaaact ataaaactct tcctttaaca tattatcatt
    37441 atacttagaa gacttcttcc ataaatcaaa tacagactta gcaatctctt ttgcatcttt
    37501 agatgtatat acagacttaa cattcatagg taaatatgca atattttcat cagttctcat
    37561 tccaaagcca gaaaatactt cagctgcatt tttcccatct atttcatgag caaatatctt
    37621 cttctcatcc atatacttag accatgcatt tgccattcca gctaaagaat ctaattctct
    37681 taaacttttc ttaccttttt cttttctata tgaattaact agattataca tttcattaga
    37741 taccatagcc ttaaactcat aactattagg gtcttttgta cttgtgttta taattacttt
    37801 gacatttaca ttaatctcac acttttgccc aaaattatca aaagcactta catttaaaac
    37861 atacgttcct gccttactgg tatccacagt tccctcaata ttaggcttca aattatttcc
    37921 ataataatca gtggcaacta tattcaacat actattatca aacttatccc ctacagaaat
    37981 ctctacatca ttagcagtga ttgtaggcat tgtattttta tcagatgcag aactaataca
    38041 ttggtcagca accttcttat ccattccacc taaagaagta acttcatcag cacctctaag
    38101 cacactctta tcacttccat cattaacaag tactataggt ttgtccttag ccaacggaga
    38161 accagccaca gcatcaacta acttataccc ttgagaaaca taaaacttct ttgttccttt
    38221 ataaaagcgt tgtataacct tcttattagt ttcaaatcta tctttaccag caatcctaac
    38281 agaattagtc ttactcacaa gttcattact cattatctcc tctgacccaa gagaataaca
    38341 ttgaacgcca tcaaccttaa atggtatact ctttccatca gtaagtataa caggaactcc
    38401 atctctagca gctacagaag atacactcat tgcatcagcc tcacctgtgt atccatttac
    38461 aagaaaaacc ttatcaccat catttactgg tttaatagct gaaatctcct tagctataag
    38521 atagctagtc tttattctat cttccccacc aattctttta acttcaatcc ctttattctt
    38581 aagttgattt tctacagatt tacctattgt atcctctgta cctataacat aagcattctt
    38641 aacatccttt agtctttttt caacatctgt tggtatctta tctctttgca ctaacattat
    38701 aggagctttt acaactccag ataatccact tgcacttaat ccatctacta tagaattatc
    38761 agtatttact aatatgactg tatcatatat ttgtttatct gctatcaatg cagaagtctc
    38821 atatctatct ttcccttgta tcttttctat actcgaaagt gcattgatat ttggtgctcc
    38881 tgccattatt aatgatattg tcaatcctat tgataatact ctcttattta ccttcatcga
    38941 actcctccta ctaatttttt tatataattc agctaatcat aaattacacc ctcagtagtg
    39001 attccctcaa aatttataaa ctactaaaac aaaaaacacc taaaatacca taacctgtaa
    39061 agcaataata aaacagatta caaaaagtac tttagatgtg attttcattt tataatatca
    39121 gtaaattaac aaaaaaacaa gatttatgga attttttaca agctaccttc attgccattt
    39181 aaaaactctt gagtagcatg cacttgacca tcagataata tataaagccc aaatccagta
    39241 ctataaaact catcactaag catgtttgca ttgtattctg gagatttctt ccacacttca
    39301 aatatagcct ttgccaagtc ttttgcatct tgagttgttt gaacactctt agaatctata
    39361 taaatatatg ctatattttc ctcacttctc attccaaact cagaaaatac ttgtggagca
    39421 tttttgccat ctatataatg ttcaaaaact ttgttatcca tcatatactt agaccatgca
    39481 tttgccatac cttctagctt actagatact accaaaggct ctttaccttt ttcttttcta
    39541 taggaattga ttaagttata catttcagta gataccattt ttttaaattc tgggctgttg
    39601 taatcatgag atttatcatc caacactttt atttctactc tttttcctgt actttttccc
    39661 cattcgtctg tagcttttaa tgtcaatatg tatgtaccta ctctatttgt atctataaac
    39721 ccatcaactt ctattggtaa taccttccca gtgttatctt tagctactat atttaacttg
    39781 ccagtgtcaa acttttcacc cttatatacc tctgtacttc caactgtaat tgctgggggt
    39841 tgtccatttg actttgaagc acttatacat tgttgtatta ccttctcatc tatttcacct
    39901 acagaggtta tattcttagc tccttctaat acagttttat cgcttccatc atttactagt
    39961 accattggag agtttttagt aagtggtgca gctgctattg catctactaa ttggtatcca
    40021 tctgatacat agaatccatc tgcactatta aaaaagtagt ctattacgtt tttgttagtt
    40081 tcaaatcggt cagttccttc tattcttact gagtttgtat ttttaactag aggattactc
    40141 attatctcct ctgaacctat acaatatgat tgaatatttt ttacatcaaa aggcacactc
    40201 tttccatctg taagtatgat tggagctcca tctctagtag ctactgatga tacactcatt
    40261 gcatctgctt ctccactata tgcattagtt agtagtactt tatcaacttt ttttatagtt
    40321 gctatttctt tagctattaa atagcttgtt tttagtctat cttctccacc aattcttttg
    40381 acttctatat ttttagaatc tagttctttt tctactgatt tacttatagt atcttctgta
    40441 cctattatat atgctttttt gatatttttt agacatctat ttgtgtctgc tggtatctta
    40501 ttttgttgtg taaatagtat tggagctttt gtagctcccg ataatccact tgcacttaat
    40561 ccatctgcaa gtgatttttc tgtatttact aaaattactg tttcataagt ctgtttatca
    40621 gctatctttg ctgctgtttc atatctatct ttaccgtata ttttttctac tactgatgat
    40681 gcatttacac ttttgaagtt tactaagatt aatgatactg ctagacctaa tgataatatt
    40741 tttttgttca ttttcatatc cccctgatga tttttatatt ttatatttat attgtgatta
    40801 ttttccattt attttctctt tattttccat atattataga gtatatatga tatgaa
//
LOCUS       DAMJYR010000026        40368 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.026, whole genome shotgun sequence.
ACCESSION   DAMJYR010000026 DAMJYR010000000
VERSION     DAMJYR010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 40368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40368
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.026"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(65..469)
                     /locus_tag="PC471_001290"
     CDS             complement(65..469)
                     /locus_tag="PC471_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3139 domain-containing protein"
                     /protein_id="HDF2788428.1"
                     /translation="MKKYKVIFIVLIIGIISVTGFVVYKNCIKPWKDAETYIDKYMVK
                     QDISKDNIKSITKEKAKKASYEGILYKVYYKDDPRYEYQYFYSDNYYALYVNKVMLQI
                     YDMENNNKLLRSNEELKSVKYPPIYLKYKPDK"
     gene            complement(661..1848)
                     /locus_tag="PC471_001291"
     CDS             complement(661..1848)
                     /locus_tag="PC471_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HDF2788429.1"
                     /translation="MSKIVFFSIPAYGHTNPTIEVVRELVDRGNEVLYYSFNEFKDKI
                     EGAGAKFICCDKYLPELLPGDEKKIGKDFPGLIEMIVDTTISLDEKVCRELKDFNPDC
                     IVSDSLSFWGKLFAQKLNIPYVCSTTTFAFNKHTAKLMKQNFIEIIRMIFGVRRINKK
                     IKLLQRKGYEVKNFISIVSNDNDTDTIVYTSKEFQPMVETFSSKYSFVGPSVSKLIIE
                     PKERKRKLIYISLGTINNKNVSFYKNCINAFKDSDVDVIMSVGRSTDIKSLGNIPNNF
                     EVKNSVEQIAILQKTDVFVTHSGMNSVNESLYYGVPMVLFPQHSEQRMVAKRVVHLGA
                     GIMLKEDKSESIKKAVFQVINDNEYKENATKLSKSFYNAGGSKKAADVILQIICNSRQ
                     VDI"
     gene            complement(1880..2305)
                     /locus_tag="PC471_001292"
     CDS             complement(1880..2305)
                     /locus_tag="PC471_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2788430.1"
                     /translation="MINENLKSLRKINQYTQEELAEKLNVSRQSIAKWESGESIPDIG
                     SCIKLAKLYNVKLDDLVNHSEEKTGIIVPPKGKFFFGAVVVGERGQIVIPKEAREVFN
                     INAGDKLLVLGDEERGLGIVHQRDLINFIGHVGVVHKEE"
     gene            2783..3532
                     /locus_tag="PC471_001293"
     CDS             2783..3532
                     /locus_tag="PC471_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440038.1"
                     /note="N-terminal region resembles flavodoxins. C-terminal
                     ferrodoxin region binds two 4Fe-4S clusters; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EFR1 family ferrodoxin"
                     /protein_id="HDF2788431.1"
                     /translation="MILYFSGTGNSRYVARKIAQELNDELISLNQLIKEEKTDELIST
                     DKPFIFVCPTYAWRLPIVVTDFIKETKFLGSNRVYFVMTCGGDTAKSINYIQKLCKYK
                     EWQLKGMAEIKMPENYIALFSTTDKETSKQMIKEADKQIYRIISDIRNENEFEIITPS
                     GLGGTIKSGIINTVFYKTIISAKGFHYTDKCIGCGKCVELCPLNNINLKNKKPVWKNN
                     CTHCMACICGCPTEAIEYKNKTQNRERYYLE"
     gene            complement(3708..4073)
                     /locus_tag="PC471_001294"
     CDS             complement(3708..4073)
                     /locus_tag="PC471_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HDF2788432.1"
                     /translation="MEKECQQRMQEITSKFKECRKAISAMGDETRQLILIALLESDFN
                     GIRVGEITKKTHLSRPAVSHHLKILKEAEIVNLRREGTKNYYYLDSKKSQWRSLTELI
                     NLIFVGIQHISENDIRKGD"
     gene            complement(4089..4865)
                     /locus_tag="PC471_001295"
     CDS             complement(4089..4865)
                     /locus_tag="PC471_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HDF2788433.1"
                     /translation="MKYQHIIEVDKELCIGCGLCKNDCPVNNIIIENKKSVIKKQDCL
                     MCGHCAAICPTKAITLTGFDEPPIELTNKPKLDSDELLMAIKSRRSIRKFKDKEVSSE
                     IIKQIIEAGRYTPSAKNSQDVSYIVLDNKKSIYENEAVKFFRKIKPIANIAIKYSKEV
                     EIDDNFFFKHAPIAIMIITKDKISGSLAASNMALMAESYGLGVLFSGYFSDVANNSPK
                     LKKLLGLKRSNHVLTTLVIGYPDVKYRRTAQKEVATVRYL"
     gene            complement(5276..6703)
                     /gene="ascB"
                     /locus_tag="PC471_001296"
     CDS             complement(5276..6703)
                     /gene="ascB"
                     /locus_tag="PC471_001296"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HDF2788434.1"
                     /translation="MSFKNDFLWGGATAANQCEGGYNEDERGLANVDVCPTGKDRTAV
                     ITGKLKMFDFDDEHYYPAKTGIDMYHNYKEDIKLFAEMGFKVYRMSIAWSRIFPKGDE
                     ETPNEKGLQFYENIFKECKKYKIEPLVTITHFDCPMHLVKKYGAWRNRKMIGFYEKLC
                     NVIFRRYKGLVKYWLTFNEINMILHAPFMGAGICFEEGEDVEKIKYQAAHHELVASAI
                     ATKIAHEVDSNNLIGCMFAAGSVYPYSCNPNDVWEATKLDRENYFFVDVQSKGKYPNY
                     ALKYMEQKGITPEMEPGDIELLNKYTVDFISFSYYNSRCVRTDENADDMAEGNIFTSA
                     KNPYLSYSQWGWPIDPLGLRITLNHVYDRYEKPLFVVENGLGAKDIADENGYVEDDYR
                     IDYIREHIKAMHDAVSIDGVDLLGYTTWAPIDLVSAGTGEMEKRYGFIYVDRDNSGNG
                     SLRRMKKKSFEWYKKVIASNGEDLY"
     gene            complement(6764..8692)
                     /locus_tag="PC471_001297"
     CDS             complement(6764..8692)
                     /locus_tag="PC471_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDF2788435.1"
                     /translation="MGKYKQLAQEIVNNVGGKENISGLVHCITRLRFTLKDESIANDN
                     VLKNMEGIVTVMKSGGQYQVVIGNHVEAVYKDVVEIIGLDDSSTSSETKKSGNILDKG
                     IDIISGIFQPVLGIMAACGMLKGFNALFVAMGLYSDVSGGYMVINAAGDALFTFLPLF
                     LGYTSAKKFGLKPMLGLALGAAVCYPAIQGSSISAGADVMYTLFKGTMFASPVYIDFF
                     GLPIVSIDYTGTVVPIIFIVYFASRCEKLFNKCVPNLVKFFFVPMLTLLITVPVALIV
                     IGPIATFGSTLISQIVISIRDFSPLLAGAIVGFTWQILVIFGMHWGFIPVYINNVMTL
                     GYDNVMMPFFACTFASSAVVLAIFFKTKDKKLKEMAIPNFISGIFGVTEPAIYGILLP
                     LKKPFIISCIASGIGGAFYGFFNLRKFITGGMGIFELPAMIEPDGGMGNLIVALSGIA
                     ISMVVAFVLTMILYKDEKVEESKKVRNKGKEEIKEVKSTKLEREIVTSPIKGEVLKLS
                     DIEDAAFASGVLGQGVAIIPSDGKVVAPVDGVVTTLFPSLHAIGILSDTGVEVLIHIG
                     LNTIQLEGRGFEACIKQGDRITKGQTILNFDVDAIKELGYSTVTPIVITNSSQFLDVV
                     ETESKNIELEDNLITVLF"
     gene            complement(8837..9679)
                     /locus_tag="PC471_001298"
     CDS             complement(8837..9679)
                     /locus_tag="PC471_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDF2788436.1"
                     /translation="MIINKILNNNVVITLDDNDEEVIVMGKGIGYQKSKGNLIDKTKV
                     NKVFRISNKEISNKLQELLNNIPIEHMKLSSEIIEYAQIKLNKKLNESIYISLSDHTY
                     SAIQRMKEGINVKNAILWEIKRFYKEEFEIGMKALDIIENKTGIKLPEDEAGFIAFHI
                     VNAQLSEGHTLASDITKLIQEVLSIVRYHFRIEFHEESVFYYRFIMHLKFFAQRLLLD
                     SAHEGETDKELLSIIKSKYNKEFECVVKIKNFIKKQYNYILTDDELIYLTIHLAKVVK
                     DSNM"
     gene            complement(10026..11396)
                     /locus_tag="PC471_001299"
     CDS             complement(10026..11396)
                     /locus_tag="PC471_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDF2788437.1"
                     /translation="MKNKYEDVAQLIIDGLGGKDNIIDLTHCMTRLRFILKDEKKVNA
                     DKLKSIDKVVGVNSTSTQYQVIIGNEVGVVFNAIINKGINTTGDIKSDIKNKNKKNSI
                     LNNIIDTITGCMTPMIPALTASGMIKVILSLCTTFNWLSSNSSTYRVLDIIGDVAFYF
                     MPIILAINAAKKFNVNTSIAVIVVGVFLHPNFSAWVSSGDPISFIGVPIQGVIYAASV
                     IPALLTVWMMSYIENFIDKLTPSMLKTILNPTLVLLISAPIALIVIGPIGNFLGEGLA
                     SIINLLQGRLGFIMVCLLAAAMPFIVMTGMHHALTPIFISAFATTGQESLILISQVCA
                     NLAQGGATLAVALRSKDSKMKQLASAAWISAIMGITEPALYGVTLKLKKPAMLASIGA
                     GIAGLFAGITHVTLYVPQNSIMAVLGLSGEKGISNIVNGLIVIFIALVAPFIMTYVFG
                     VEKNVK"
     gene            complement(11435..12106)
                     /gene="pgmB"
                     /locus_tag="PC471_001300"
     CDS             complement(11435..12106)
                     /gene="pgmB"
                     /locus_tag="PC471_001300"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HDF2788438.1"
                     /translation="MKAVLFDLDGVITDTAKYHFEAWHEIAKSLDIEIDETFNEKLKG
                     INREESLDKILLSANKVDSYTKEEKLKVCEEKNNIYIKLINSLTYKDILPGINELIKE
                     LKKNNVKLGIASASKNAPRILESLGVKDEFDCIVNPSLLKRGKPYPDIFIEGSKMLNL
                     KQYDCVGIEDSYSGIKAINAANMTSIGVGKKEVLGKCDHIVEDTSLLNYNTLLQAWSK
                     KINLD"
     gene            complement(12108..14498)
                     /locus_tag="PC471_001301"
     CDS             complement(12108..14498)
                     /locus_tag="PC471_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HDF2788439.1"
                     /translation="MSLIFTTKDQLVELGFSKDTIKSNETLFTLANGHLGVRGNIEES
                     DFNNEYVDNMGTYVNGFYEESPITYGESAYGYAKQNQTICKLPNAHIVNFSIEGDCFD
                     LSKGITSEHTRILDLNEGILKRSFIWENSRGRKVKVSIERLVSQDIEELMMISYTITP
                     LNFNGKIKFKNKMENSMEKFNCEKVDDPRVASINKKQFKINVKKYNNKYFMNLVTERS
                     RLGLWCGFDSYITCGNITNETYNVSDECIEREIEVYAKKDERLNLEIVVGYSRVYSDL
                     NLNEERLKSLECLESLKCLESEAHLKSLELVLREACNIGFDKVKNIQRKKMNEFWTTS
                     DVEIEGDKTLQLGIKTNLFHIYQSAGRDGKTNISAKGLTGDGYEGHYFWDTEMYILPF
                     FIYTQPSIARALLDYRYSILDASRERARELGINKGCLYSWRTINGNECSAYYPAGTAQ
                     FHINADIAYGVRTYFEATNDEEFMMQNGLEILIETSRFWVEFGDYIESKNNKFCINGV
                     TGPDEYTAIVNNNYYTNLMAKYNLIYAVQMVDKFKHNLFSNEVFRKVKLQEYEVKNWI
                     NAAQNMYLPYDEEKKISKQDDSFLEKSVWDFKNTPKEKYPLLLHYHPLTIYRYQVNKQ
                     ADTVLSALLFPDEFSLEQKKRDFEYYEKITTHDSSLSRSIFGMMASEIGNHEKAYNYF
                     MDTALMDINNLQKNTSDGIHAANMGGTWMSLVYGFAGMKIKNDKLSFEPRIPKHWKNI
                     KFNILFRDNLVFVNIEEYKSYYKLIKGRNITILHDNKEIKLSKNIIEVNNCFKGSN"
     gene            complement(14524..15972)
                     /locus_tag="PC471_001302"
     CDS             complement(14524..15972)
                     /locus_tag="PC471_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HDF2788440.1"
                     /translation="MKLGKDFLWGGAISANQSEGFYLDGGKGLSQFDKLPLNDKRLKN
                     VCLDEDNILNENNQYYPSHIGIKFCENYKEDIAMLAEIGFKCFRFSISWSRIFPTGEE
                     SEPNEKGLQLYDNIIKELKKYNIEPLITISHFDIPYSLVEKYSGWADRRVVNLYVKYA
                     EILMKRYKDDVKYWIPFNEMNMIMHIPYIGAGLTFIADENQLQKKYQAAHHQLLANAK
                     TIEIGRYINKEFKFGCMLAAGKTYPYTCKPEDVFATLEHERHNLFFSDVQVRGVYPSF
                     MSYYLKSNQIKLIIKEEDLSTLKDNTVDFVSFSYYSSACSSARAEGLEKSKANGPETI
                     KNPYLPKSNSVWQNDPVGLRITMNQLYERYQLPLFIVENGLGTQDIVNEDGKIIDDYR
                     IDYMKEHIKNMILGVNEDGIELIGYLMWGCIDVTSVSEGKMTKRYGIIHVDSDDYGRG
                     SFKRTKKQSAFWYKKVIETDGEILFNDMETHA"
     gene            complement(16377..17264)
                     /locus_tag="PC471_001303"
     CDS             complement(16377..17264)
                     /locus_tag="PC471_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HDF2788441.1"
                     /translation="MAIYLEMPKLIHEFPFRTLSNEGEVLTTPHWHKEIEILLITEGV
                     VNLFINDKPMQLKKGEIVIIKGGDIHYILPSPGSERLVFQFDISFFNDLILLNEEKES
                     LINLFSSIKKCSRDWSAKIRKSVFDILIDIYNEDLYKIEGYSYAIKGKMYNLIPILYR
                     QIPREIEENTVEYEINSKEILERLNNIFKYVEKNYKQPIKLEDVSYEVGFSVYYFTKF
                     FKKNTGKTFITFLNEYRLEKAKWILLNNNISIVDLVEEVGFGSTKTFYRVFKEKIGVS
                     PLEFKKRYNSLEKKGSVSK"
     gene            complement(17364..17813)
                     /locus_tag="PC471_001304"
     CDS             complement(17364..17813)
                     /locus_tag="PC471_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDF2788442.1"
                     /translation="MEIREILTEESIILDLKSTTKEGIIQELVDKLYDTGSITLKSKF
                     IKALKHREKIGSTSIGYQMAMPHGKTKYVKKASIVIGKSKKGVDWPSIDGLPVKVIIM
                     FAMPQSGGNEDMILISKIAYILGGKNIGERLLNAHTKKEILDIFYEV"
     gene            complement(17803..19710)
                     /locus_tag="PC471_001305"
     CDS             complement(17803..19710)
                     /locus_tag="PC471_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HDF2788443.1"
                     /translation="MLVSRQIEFLKLILNENKYRPIKYFKDKLNVSDKTLQKDLKMIE
                     KYLETFNIKIDIKRGYGILIEDLAKKNIDLINSLNIQSKENKSLSVEQRRMEILKYLL
                     LNSNNITSINQLADKYYVSKTSIVNDFKYIEAWIKEYNLKLNKTLEGTKIIGKEVDIR
                     KSIAKMMDDLLDENYEQHDISELTRLDSATFSALINLFDIDSIIFVETIITELEYNLG
                     YTINQPYYINLITHILICLKRIEEGNQIESQEEREISVDNLDELVYKNVTTLIDKIEN
                     RYRVKITKDEIMYIYIFLVSSGFSSENNSSYNDESDRLSESEKVSSIMIENMSRFLNI
                     NLKNDDLLQKSLASHVRPMLNRLRYDIQIKNPLLGEIQERFSDVLGLCMMTINIMTEY
                     DNLKNISIDEVSYLATYFQAAIERNMSSKRVIVVCHTGYGTSQLLAARLKREFPEWTI
                     VDIVPMHQLSKRNLDDVDFVISTVNLDLKDKLHIVVSVLLMESDINNIKNALLSKPKK
                     SMNLDTNLLGMYLEDNIYFNFDVAQMESLIGVNLGSKYESISLGKDLNIYIHKQSKLN
                     KGIMNINNVTRTIDIYLEISNQSFLKNILMEISNLYRQKNYINYIIDCKSKEDIKILF
                     KNNLLEKTNGN"
     gene            complement(19978..21873)
                     /locus_tag="PC471_001306"
     CDS             complement(19978..21873)
                     /locus_tag="PC471_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDF2788444.1"
                     /translation="MIGKILRSDCIVLDKQLQTKKDVINYMANHLYHGNYINNKEKFI
                     ADINKREKESSTNMGNLIAIPHAKSNVVNQPVMMYLRTDREITDNDGQKLRSFFMIAV
                     PESGSDEHLKIISKLATYLMEENFLKHLLSAETPYEILSIFNNKAAESGDFSNNQNKG
                     LIVGITGCPTGIAHTFMAKKALEEAGQELGYEVKIETHGAIGVENKLTKEDIKRAEYV
                     IIACDKEVQLSRFIGKKVLQKKVVDAKNVSLAKNLIINAENGMAKEIKPGGKTLEDSK
                     EMLQGSEKKGVYKHLMSGVSEMIPFVVIGGIGIAIAFMFGIFASDPTHETYNPIAGFF
                     GQLGGDSAFKLFIPILAGFIAKSIAGRQGFAPAMIAGFMAVIGGSGFIGGMVAGFATG
                     YIALGVMKITENLPDVLKGVNAVLICPLLIAFTSGAIMFFVVNTPASWLNEALQSWLQ
                     SMSGTNKVLLGAILAGMMASDMGGPINKTASAFGLAMFSSNIFEPSAALMVGGMVPPL
                     AISLAMLIFKNKFTKEERKSSLPTAIMGASFITEGAIPFAAADPLRMIPANIIGSCIG
                     GAICMSLDITLMAPHGGIFVIPFACSAPVAYIACILIGTVITALIIGVTKPTIRSENS
                     EVELLGA"
     gene            complement(21909..22787)
                     /locus_tag="PC471_001307"
     CDS             complement(21909..22787)
                     /locus_tag="PC471_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HDF2788445.1"
                     /translation="MLINMKEMLKVAQENQFAVPAFNIGSGQILKAVVQSANEKNAPV
                     ILAIHPNELSFLGDSFVASCIEEANKSKVPMVIHLDHGENKEQILRAIRCGFTSVMID
                     GSHLPYEENVAISREIVEIAKGLNVSVEGELGTIGTTGTSSEGGTDEIIYTDSKLAKD
                     FVEKTGVDTLAIAIGTAHGIYPKGFKPELKLDLLKEIREVVDIPLVLHGGSSNPDEEI
                     AQAVKLGVCKINISSDVKSAYYKKCRELLEQNPSLYEPDTIYPPCIKAAREVIEFKMN
                     LFNAIDKLKCFYKNKN"
     gene            complement(23330..23500)
                     /locus_tag="PC471_001308"
     CDS             complement(23330..23500)
                     /locus_tag="PC471_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2788446.1"
                     /translation="MSALLYGVCNKSISVSIVINMQEFKIGVSMAYGVLVIGICYIAL
                     GIIFKLDRKRFT"
     gene            complement(23897..24109)
                     /locus_tag="PC471_001309"
     CDS             complement(23897..24109)
                     /locus_tag="PC471_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2788447.1"
                     /translation="MAILMKTKIKEYREKLLMTQNELAKLVGVRRETIVHLENGKYNP
                     SLKLAMDIAKVFDTTVENLFEFIEEE"
     gene            complement(24110..24526)
                     /locus_tag="PC471_001310"
     CDS             complement(24110..24526)
                     /locus_tag="PC471_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2788448.1"
                     /translation="MSKSETGVRSKLILELLVITYFFIAVLATKYIFKGPSTASLPGL
                     SWLIVFGCYSIYNKKYNDLVDESSEFILSKVNKIAIKIMIYSIVIISIFFATPYTSNI
                     NISNLDTGIILIAVLFIQSLSKLVLYIYFDKRGICN"
     gene            complement(24594..25007)
                     /locus_tag="PC471_001311"
     CDS             complement(24594..25007)
                     /locus_tag="PC471_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2788449.1"
                     /translation="MSKSELGVKTNLVSELLMISYFFIDSLLKKFIFKEGNLCITFII
                     LFAFWACDFMYNKKYNDLVDEASKLMLLKVDHIASKIMLYSILLIAVFFSANYTRGLD
                     ISNLDIGVILLAILFIQSLSKLILYIHFDRKGICN"
     gene            complement(25840..26469)
                     /locus_tag="PC471_001312"
     CDS             complement(25840..26469)
                     /locus_tag="PC471_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HDF2788450.1"
                     /translation="MKNRKVYMYVFDTMSDWEVAYLTAELNTGRYFKKGLKPLKVITV
                     GIDKNPIITMGGLKVLPEITVDEFNMESKDLLILPGGNTWLSMNHEIILEKVKEALRQ
                     DSIVAAICGATLGLAKKGLLDFRNHTSNDLEFLKMVIPNYSGEKYYKTESVVSDENLV
                     TASGIAPLEFTFQVLKLLDVFESEALNSWLNLYKTHDSKYFFELMNCNK"
     gene            complement(26548..27510)
                     /locus_tag="PC471_001313"
     CDS             complement(26548..27510)
                     /locus_tag="PC471_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017561613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HDF2788451.1"
                     /translation="MAKIDNMMSILWMLNSDKKITAKQIAEKLEINIRTVYRYIDVLS
                     ASGVPIISDTGHNGGYSLLNNFTKAPLFFDIEEQKSLLHAALFAKKEGYFLDEALDKS
                     ILKLKMYSNQKQEDILTNHLRALEVIKPIGKVSIESVLKKLENSILKELSVEINYHAG
                     RKVKSKYRIINPYGIVHWNNNWYVVAFCNMRNEVRSFRVDRISEIIETSNTFNRPTEF
                     SASEFFIKGIIPEIKDEQNIIQLVIEGSIDSLDSLSQHWFLGHYLKERTPNKIIFMLE
                     EEVICKYIPNILLPYRKTIQVIEPLSLRKKIIEDLLELIDYYQI"
     gene            complement(28272..31787)
                     /gene="cbpA"
                     /locus_tag="PC471_001314"
     CDS             complement(28272..31787)
                     /gene="cbpA"
                     /locus_tag="PC471_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding adhesin CbpA"
                     /protein_id="HDF2788452.1"
                     /translation="MQQKCKKKALSMILSISMGLGPVASFNASNTFADTIEESTNAGN
                     AEGGTNAGNAEGDTNAGDAEGDTNAGDAEGDTNAGDAEGDTNAGNAEGDTNAGDAEGD
                     TNAGNAEGDTNAGDAEGDTNAGDAEGDANAVVVEEEATAEEPTSVGDFKISGGEINVD
                     FKFASGVLTILSGKQMTIQNNNPNTPTTHRIVIPKDVKAELIFDGVNIKTASNSPFTL
                     TPDKDGGAYAHVILQDGSENKLESGDVYYPGMRAGKGTTLIIDDSVVNRDIEGEFIVP
                     EGGKIPRDVTLENGDKLKKGDRLTLLDSNNRGSLKVVGNYYSAGIGGGNTESGGNIII
                     NGGAITAIGQESSRNTGYGAGIGGGGNGSGGDITINGGIITAKGAYHGAGIGGGYYTT
                     TKNALSPSVVDPSTGSGYSGNITINGGLSYSYGGAHGSAFGDGCVNPGTNRGYRILVT
                     GGTILPYMTGVGDWIFDLSATTGDVIVTGGSLKATTFKSLGGAVAYGDLEKKTKVFMN
                     KISLTYLGQDKVSTTLVDDFKMLIDGKIYPYGAPSYTDAQGILYLWFSDQFKGSEVSV
                     NLVVIDKITGEVLKPDDFYIKDIGSGSQFLKQYEEFTIDDTPPYLIIKRYDGLPFEED
                     VEKSFLDWIFKDGGIPIITPKGEKLTDKKFMTISSQRLNEETMEPDINLGSDDLTKQV
                     DAGKYQLSITSTQFSSIYPFKDAYWGHRAYYKAEIIPADTETSLVVEESKAKTDVFKP
                     TDTFTLNVTISPDKKEGPDCASPRGYVQFYINGKEYKDPVELKTHNKGSESHNYSTAS
                     IEWRPLDSNHHIFTTEQTITVKYIGDDINYIEGSEDEKKLKLDMTNVDVDGDGIPDIN
                     IDTDGDGRPDINIDTSGDWKPDINIDTDNTGEWKPSTEGGNGDGIWKPDKNIDTNGDG
                     NPDTDYNRPAIDTDNDGVDDYWKPDKNVDTGSGGYDTGNPNLNKPDPDNGGNSKPDSG
                     NGGNSKPDSGNGGNSKPDSGNGGNSKPDPDNGGNSKPDPDNGGNGKPDPDNGGNSKPD
                     PDNGGNSKPDPDNGGNSKPDIDTDGDGKPNINIDTDGDGKPDINIDTDGDGKPDINID
                     TDGDGKPDINIDIDGDGKPDINIDTDGNGKPDINIDTDESLDSNVGQNVQTGDQSNIM
                     LDLALMFISLFFLIKNLTNKYLRRK"
     gene            32056..32628
                     /locus_tag="PC471_001315"
     CDS             32056..32628
                     /locus_tag="PC471_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class B sortase"
                     /protein_id="HDF2788453.1"
                     /translation="MKKLVKILINIICIITLIFSSLSIYIKLSEYKKADEVYTELREN
                     TINNSKHQELYDKNNDYRFWLKINNVNIDYPVVQGYNNDFYLTHDFYKNYLPLGSIFM
                     DYRNNFENDKSLIVYGHYMKNKTMFGQLENYTDEAFFKENNLVEINYKVQTYTYEIFS
                     VYTADLINRDYLSIHFNSNYEFKDYLNYIT"
     gene            complement(33641..34303)
                     /locus_tag="PC471_001316"
     CDS             complement(33641..34303)
                     /locus_tag="PC471_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2788454.1"
                     /translation="MKMLKVIDIKKIYGKGENEIKAVDGITLEIEPHKFSAIIGQSGS
                     GKSTLLHCMAGLDKPTLGNVFMDNLDLYTLSDEKLSNLRSKEFGFIFQNYNLIPVINV
                     YDNIVLPILIAKGKIDNNYINDLITKLGLESQINKFPNELSGGQQQRVAIARSLANKP
                     SIIFADEPTGNLDSKTTNEVMNLLKYCVNEYKQTLVMITHNDEIAKMADCIITISDGK
                     VI"
     gene            complement(34320..36875)
                     /locus_tag="PC471_001317"
     CDS             complement(34320..36875)
                     /locus_tag="PC471_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HDF2788455.1"
                     /translation="MGIYLKMSLAYLKKNKLRTLLLIIGVMLGVMLIFGPSVIKDSQN
                     ENNVVAIHELYSGYHVEFSDLTLDDAKKLETDEKVSKGNSVQNLGRISDKKGNSFILT
                     SSNEEYLKSRYNKIIEGRLPKNNNEIVLEKKALEAMNLSEGVGSTLNLTIKKNYKDTN
                     GSNQLFSINKEFKIVGVMEKPKGYYDVVWQYEAVTYGNDETNNIIPHDAISYNSILSF
                     KSGWRNIEGECDYLMKKYKLGQKSFLPNVPLVRELRYVEDDKNDPNVFKRNVLITTVS
                     IIFIFNIFNITLNQSVKEMGLLRLMGAKKRNIRCMIIYQALIITIIGILLGLVAGVTY
                     SYIGISNYNEVLYSEAGLNPKLYINNEIVIKAILVGMISVFISCIIPTFKIGKISPIE
                     SIINTDKIKNHRSRLGIDRLVSKIFGFYGFMGLKNIGRNKVRAAISMLSIALGGYIFI
                     TTFSSMQEEVNSKIEDMQNRYNITMQFGVNADPQISKYTDSDVENIEKIKGVKSIHKI
                     QITNGMFTFNNKDINKEFTTYNGVEKKEIMEDKLVLKLYDDDYIDEKLNDFVEEGSLD
                     DIKKDTDGYPNVAVYNYFYDIVDDHTIKPIFKELNIGDIITVKIPVEENNTIVYKEMK
                     VRVAVILKDEWIAMGDGDFMPDLEIITSNIHSKNITGEQKYTQLGINLENPYDEIVNN
                     EIQKISRSIPGSLFNSRLSYHEMSESIYKSYIKSQIAIIALVLIIASINIFCTIRTNL
                     LIRKKEISTLRALGVSLKNMKKMLIYEALAYAVFSFIIAMIPSFINLVKFTNWNNDAY
                     INYGIENFMSFTFPIKESLLFFAISVFVCLISVVVSNRDFESMNIIEGIKENE"
     gene            complement(37115..37303)
                     /locus_tag="PC471_001318"
     CDS             complement(37115..37303)
                     /locus_tag="PC471_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3139 domain-containing protein"
                     /protein_id="HDF2788456.1"
                     /translation="MRKYKAILLTLIISIIAIIGFIIYNNYFRKWNEAEKYIDKYMTY
                     QGVSGDDIERITKEKYTK"
     gene            complement(37315..37494)
                     /locus_tag="PC471_001319"
     CDS             complement(37315..37494)
                     /locus_tag="PC471_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2788457.1"
                     /translation="MIHIEISEKEFFRKIEEVFDKGEVNVEVDNLGDIKIQVKKFDDK
                     TKSLELKLIKEFKLK"
     gene            complement(37499..37756)
                     /locus_tag="PC471_001320"
     CDS             complement(37499..37756)
                     /locus_tag="PC471_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2788458.1"
                     /translation="MSKKISESIENSGLRVAENKSDGSFVLSLGNLEYTKEQPSQVLH
                     YSITKEKKDTKEILSIQCVKDFSYNEKLSEDDKFIMAIYNI"
     gene            complement(38016..39409)
                     /locus_tag="PC471_001321"
                     /pseudo
     CDS             complement(38016..39409)
                     /locus_tag="PC471_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428529.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
     gene            complement(39403..40078)
                     /locus_tag="PC471_001322"
                     /pseudo
     CDS             complement(39403..40078)
                     /locus_tag="PC471_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438019.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
BASE COUNT        13087 a         6880 c         4194 g        16207 t
ORIGIN      
        1 atttaaacgc tctagtgata tttttaaata aattttaata aaaattattc tattttgtca
       61 taaatcattt atctggctta tatttaagat atattggtgg atactttaca ctttttaatt
      121 cctcattact tcttaaaagt ttattattat tttccatatc ataaatttgt aacattactt
      181 tatttacata tagagcataa taattatcac tataaaaata ttgatactca tatcgagggt
      241 catctttgta atacactttg tatagtattc cttcgtaact tgccttcttg gctttttctt
      301 ttgtaatact ttttatatta tcttttgaaa tatcttgttt taccatatac ttatcaatat
      361 atgtttcagc atctttccat ggtttaatgc agtttttata tacaacaaac cctgttacac
      421 ttataatacc tataattaat actataaaaa taactttata ttttttcata ttaatcctcc
      481 ttcatttatt atttaaaact cgatataatg cactaaaaaa aggatgttat cttttttatt
      541 aaaatataac atccctcata tttagcatca atatatatgc cccaactggt aggttaaaag
      601 accccactgt cagtactttg attaaattac atgtatgtat atatattttt aacaaatcta
      661 ttatatgtca acttgtctac tattacatat aatctgtaat attacatctg ctgctttttt
      721 tgagcctcct gcattataaa agctttttga tagtttagtt gcattttctt tatattcatt
      781 gtcatttata acttgaaata ctgctttttt aatactttct gatttatcct cttttaacat
      841 aattcctgct cccaaatgaa caactctctt agctaccatt ctctgctccg aatgttgagg
      901 aaataatacc ataggtactc cataatataa actttcatta acactattca tcccagaatg
      961 agtaacaaac acatcagttt tttgaagtat agcaatctgt tcaacactat ttttaacctc
     1021 aaaattattt ggtatatttc ctaaactttt aatatcagta cttctaccaa cagacataat
     1081 cacatcaaca tcagaatctt taaatgcatt tatacagttt ttataaaagc taacattttt
     1141 attattaatt gtaccaagcg aaatatatat tagctttctt tttctctctt taggttcaat
     1201 aatcaacttt gatactgatg gacctacaaa tgaatactta ctagagaagg tttctaccat
     1261 tggttgaaat tcttttgatg tatatacaat ggtatccgta tcattatcat tagaaactat
     1321 agaaataaaa ttttttactt catacccttt tctttgtaaa agctttattt ttttattaat
     1381 gcgtcttaca ccaaaaatca tacgtataat ttctataaag ttttgtttca tcaactttgc
     1441 tgtatgttta ttaaatgcaa aagttgttgt agagcataca tatggaatat taagtttttg
     1501 tgcaaataac tttccccaaa aacttaatga atctgataca atgcaatctg gattaaaatc
     1561 tttaagttct ctacaaactt tttcatctaa agaaatagta gtatctacta tcatctcaat
     1621 taatccagga aaatccttac caattttttt ctcatctcca gggagtaatt ctggaaggta
     1681 tttatcacaa caaataaatt ttgctccagc accttcaatt ttatctttaa attcattaaa
     1741 agaataatac aatacctcat ttcctctatc cactaattca cgaactactt caatagtagg
     1801 attagtatga ccatatgcag gaatgctaaa aaatactatt ttactcatat ttcatacctc
     1861 cttttataat ttttttaaat cattcttctt tatgtactac tcccacatga cctataaaat
     1921 ttatcaaatc acgttgatga acaattccta atcctctttc ctcatctcca agtaccaata
     1981 atttatctcc tgcatttata ttaaatactt ctcttgcttc ttttggaatg acaatctgac
     2041 cacgttcacc aactacaact gctccaaaga aaaactttcc tttaggaggt actataatac
     2101 ctgttttttc ttccgaatga ttaactaagt catctagttt aacattgtac aattttgcaa
     2161 gtttaatgca actacctata tcaggaatag attctccact ttcccatttt gctattgatt
     2221 gtctagaaac attaagtttt tcagctaatt cttcttgtgt atactgattg atttttctta
     2281 gactttttaa attctcatta atcatatttt tcatacctcc tatgtttata atattggatt
     2341 tattatttaa attctatcaa ctaatcttaa cattcatata tatatttata tacttttatt
     2401 atataaattt attgttcagg taacatattt ccttaagtta tcgtaaacaa aaaaacaaac
     2461 acaaatctac taactgttgt taaagtattt aagttaaggt catttacatt atcaaatatt
     2521 tctacacctt caaaataacc tttatattta taatctttca taagcaaatt attatccctt
     2581 ctcatataca agtccagttg atgtcttgtt gctaaattta atggtaatca aattctttac
     2641 caataagtat aaaaattact aatgtgttat aattttctta actactaaat tttacataca
     2701 tttgaataga atatatcaat aaattactat aataaataaa aaattaagag ttaaaataaa
     2761 aaatagttag ggagatatta ttatgatttt atattttagc ggaactggta atagccgata
     2821 tgtggctcgt aaaattgcac aagaattaaa tgatgaactg atatcactaa atcaacttat
     2881 aaaagaggaa aaaacagatg aattaatatc aacagataaa ccattcatat ttgtatgtcc
     2941 tacttatgca tggagattgc caatagtggt tacggatttt ataaaagaaa caaaatttct
     3001 aggaagcaac agagtttact ttgtaatgac ttgtggtgga gatacagcaa aatccataaa
     3061 ctatattcaa aaactatgta aatacaagga atggcaatta aaaggtatgg cagaaattaa
     3121 aatgccagag aattatatag ctttattttc tacaacagac aaagaaacat caaaacaaat
     3181 gattaaagaa gcagataaac aaatatatag gattatttct gatatacgaa atgaaaatga
     3241 atttgaaatt attactccta gtggcttagg aggtacaatt aaaagtggta taataaatac
     3301 agttttttat aagacaatta ttagtgccaa aggatttcat tatactgata aatgcattgg
     3361 ctgtggaaaa tgtgtagagc tatgtccttt aaataatatt aatttaaaaa acaaaaaacc
     3421 agtttggaaa aataactgta ctcattgtat ggcatgtatt tgtggttgcc ccactgaagc
     3481 tattgagtat aaaaataaaa ctcaaaacag agaaagatac tatttagaat aatatattct
     3541 taatatttaa accattataa aatgaactat acccattgtt ttatacagtt ttacatattt
     3601 taactgtctc ctaagatggg tatagttcaa aagatagtgt ctcaaagtga actttttagt
     3661 tctttttagt tcctaaatca atctttttgt attaaatcaa gtataattta atcacctttt
     3721 cttatatcat tttcactaat atgttgtatt ccaacaaata taagatttat tagctctgtt
     3781 agtgacctcc attgtgattt ttttgaatca agataataat aattttttgt tccttctcta
     3841 cgcaaattaa caatctctgc ttcttttaat atcttaagat gatgagatac agcaggacga
     3901 gaaagatgtg ttttttttgt gatttctccc actcgtatac cattaaaatc actctctaag
     3961 agagcaatta aaatcaattg tctagtctca tcacccatag cagagatagc tttgcgacat
     4021 tctttaaatt tagacgttat ttcttgcatt ctttgttgac attctttttc cataaaatcc
     4081 accctctctt ataagtatct aacagttgct acttctttct gtgcagtacg tctatattta
     4141 acatctgggt atccaattac taatgttgta agaacatgat tactgcgttt tagacctaaa
     4201 agctttttta attttggcga attatttgct acatctgaaa aatatccact aaataaaaca
     4261 cctaggccat aagactctgc cattaatgcc atatttgatg ctgcaagtga accactaatc
     4321 ttatcttttg taattatcat tattgcaata ggagcatgct taaagaaaaa gttatcgtca
     4381 atctcaactt cttttgaata tttaatagct atatttgcaa taggttttat ttttctaaaa
     4441 aatttcactg cttcgttttc ataaattgac tttttattgt ctaaaacaat atatgataca
     4501 tcttgtgaat ttttagcact aggggtatac ctacctgcct caataatttg ctttataatt
     4561 tctgaagaaa cttctttgtc tttaaattta cgtatacttc ttctagattt aattgccatt
     4621 agtaattcat ctgaatctag ctttggctta tttgtaagtt ctataggagg ttcatcaaat
     4681 cctgtcaagg taatagcttt tgtgggacat attgctgcac aatgtccaca cattagacaa
     4741 tcttgttttt taattactga ctttttattt tctataatta tattgtttac tggacaatca
     4801 tttttacaaa gcccacatcc aatgcaaagt tctttgtcaa cttctattat atgttggtat
     4861 ttcatattca ttcatctacc tttcgtttat tagtttaaac tattataaca cgtagacatt
     4921 ttttatattc attggtaaag aatttgatta ccattaaatt tttaatttct ctattaaata
     4981 aatttttcat agaattttaa agtatcatag tttatacata ataaaaaaga caagcttaaa
     5041 acctaagctt gcctttttca tcttttatta caaagtaaat tgcacttact tcccatactt
     5101 cttaacaatt ttattttcta aaattttatt tgtgaatcat actattgcat aaatacaatg
     5161 taaaataatt ctgtattgat tttctatagt taaaaaagtt aaaaattata cttattatta
     5221 atttttatac tcttcttatt taatacaaat ttcttattta acatagattt cttgtctaat
     5281 ataaatcttc accatttgaa gcaattactt tcttatacca ttcaaaactt ttctttttca
     5341 ttcttcttag acttccatta ccactattgt ctctatctac atatataaat ccataacgct
     5401 tttccatttc acctgtacca gcacttacta aatcaatagg tgcccatgta gtataaccta
     5461 ataaatcgac cccatctata gaaactgcat catgcatagc ttttatatgt tctcttatat
     5521 aatcaattct gtaatcatct tctacataac cattttcatc agctatatct ttagcaccta
     5581 atccattttc tacaacaaat agtggctttt catatctatc atatacatga tttagtgtaa
     5641 ttctcaaacc taatgggtca attggccatc cccattgact gtatgaaaga tatggatttt
     5701 tagcagatgt aaatatgttt ccttcagcca tatcatctgc attttcatcg gttctaacac
     5761 aacggctatt gtaatatgaa aatgaaataa aatctactgt atatttattc aataattcaa
     5821 tatcaccagg ttccatttct ggtgtaatac ctttttgctc catatacttt aacgcatagt
     5881 taggatattt acctttagat tgtacatcca caaagaagta attttctcta tctaactttg
     5941 tagcttccca tacatcatta ggattacaac tataaggata tacacttcca gctgcaaaca
     6001 tacatccaat taagttattt gaatcaactt catgtgctat tttagttgca attgcactag
     6061 ctacaagctc atgatgggca gcttgatatt ttatcttttc aacatcttca ccttcttcaa
     6121 agcaaatacc agcacccata aatggagcat gtaaaatcat atttatttca ttgaaagtta
     6181 gccagtactt cactaatcct ttatatcttc taaagataac attgcataat ttctcataga
     6241 aacctatcat ttttcgatta cgccatgcac catacttttt aactaaatgc attggacaat
     6301 caaagtgtgt aatagtgact aaaggctcta ttttatattt tttacactcc ttaaatatat
     6361 tttcataaaa ttgaagtccc ttttcatttg gtgtttcttc atcacctttt ggaaatattc
     6421 gactccatgc aatgctcatt ctatatactt taaatcccat ttcagcaaat aactttatat
     6481 cttctttata attatgatac atatcaatac cagttttagc tggataataa tgctcatcat
     6541 caaaatcaaa catttttagt ttccctgtaa taactgctgt tctatctttt cctgttggac
     6601 atacatctac atttgccaat cctctttcgt cttcattgta tccaccttca cattgattgg
     6661 ccgcagtagc tcctccccat aaaaaatcat tcttaaaact catagtcaaa gcctcctaaa
     6721 attataacca aagcactaga ttatgctaat acctcaatct ttactaaaat aagactgtaa
     6781 ttagattgtc ttctaattca atatttttac tttctgtttc tactacatct aaaaattgtg
     6841 atgaatttgt aataacaatt ggagttacag tactataccc caactctttt attgcatcaa
     6901 catcaaagtt taatatagtt tgaccttttg taattctatc accttgtttt atacatgcct
     6961 caaaacctct tccctctaat tgaatagtat ttaatccaat atgaataagc acttcaacac
     7021 ctgtatcaga aagaatccct atagcatgta gactaggaaa taaggttgta actaccccat
     7081 ctactggagc aactactttt ccatcactag gtattattgc caccccttga cctagtacac
     7141 cagatgcaaa tgctgcatcc tctatatcac ttaattttaa tacttcacct tttataggac
     7201 ttgttactat ttctctttct aatttagtag acttgacttc ttttatttct tctttaccct
     7261 tatttcttac ttttttagac tcttctactt tctcatcttt atataaaatc atagttaata
     7321 caaaagctac gaccattgat atagctatac cagaaagagc tactattaag ttacccattc
     7381 ccccatctgg ttcaatcatt gctggaagtt caaatattcc cattcctcca gttataaatt
     7441 ttctaagatt aaagaatcca tagaaagcgc ctccaatacc actagcaata caacttatta
     7501 taaatggttt ctttaaaggc aataatattc cataaattgc tggctctgta actccaaata
     7561 taccagatat aaagtttggt attgccatct cttttagttt tttatcttta gtcttgaaaa
     7621 atattgccaa cacaactgcc gaacttgcaa atgtacaggc aaagaatggc atcataacat
     7681 tatcatatcc taaagtcata acattgttta tatatacagg aataaatccc cagtgcatac
     7741 caaaaataac aagaatttgc caagtaaatc ctactatagc acctgccaat agtggactaa
     7801 aatctcttat tgaaattaca atttgtgaaa taagtgtaga accaaatgtt gctatagggc
     7861 ctataactat aagtgcaaca ggcactgtta ttaataatgt aagcattggc acaaagaaaa
     7921 acttaactaa atttggaaca cacttattaa ataatttttc acacctagaa gcaaaataca
     7981 ctataaatat aattggtacg actgttccag tataatcaat tgatacaatt ggtagtccaa
     8041 agaaatcaat atatactggt gatgcaaaca ttgttccttt aaataaagtg tacatcacat
     8101 ctgctcctgc tgatatactg ctaccttgta tagcaggata acatactgca gctcctagtg
     8161 ctaatcccaa cattggtttt aatccaaact tcttagctga agtatatcct aagaacaaag
     8221 gaaggaatgt aaataatgca tctcccgctg catttattac catataacca ccagatacat
     8281 cactatataa tcccattgct acaaataatg cattaaatcc tttcaacata ccacaagctg
     8341 ccattattcc taacactggc tgaaaaattc ctgatattat atctatacct ttatctagta
     8401 tattacctga ttttttagtt tctgaacttg tacttgaatc atctaaacca attatttcaa
     8461 ctacatcttt atagacagct tcaacatgat ttcctataac tacttgatat tgtcctccac
     8521 ttttcattac agtaacaata ccttccatgt tttttaatac attgtcattt gctatacttt
     8581 catcttttaa agtaaaacgt aatcttgtaa tacagtgtac taatccacta atattttctt
     8641 ttccaccaac attgtttact atctcttgtg ctaattgttt gtattttccc atttttctcc
     8701 tccatatttc gtttaaatta attttttgaa atttgaaggt ttggccaact caatgttacc
     8761 atccaaccat aagtataatt ttgttagtat cactcctttc aatcattctt aatatactgc
     8821 tgaaaaatta tttcacttac atatttgagt ctttaactac tttagctaaa tgtattgtta
     8881 aatatataag ctcatcatct gttaatatgt agttatattg ttttttaatg aaatttttaa
     8941 ttttaactac acattcaaac tctttattat atttagattt aataatgctt agtaattctt
     9001 tgtctgtctc accttcatgt gcactatcta aaagcaatct ttgcgcaaaa aactttaaat
     9061 gcattataaa tctataataa aacactgatt cttcatgaaa ttctatccta aaatgatacc
     9121 taactatgct aagtacttct tgtattaact tcgtaatatc agatgccaaa gtatgacctt
     9181 cacttaactg agcattcact atatgaaagg ctataaatcc agcctcatct tctggtaatt
     9241 ttatacctgt tttgttttca ataatatcta gtgctttcat tccaatttca aactcttctt
     9301 tataaaatct tttaatttcc caaaggattg cattttttac atttattcct tctttcattc
     9361 tttgaattgc agaatatgta tggtcactta aggatatgta aatactttca tttagcttct
     9421 tgtttaactt tatttgtgcg tattctataa tttcacttga taatttcatg tgttctatag
     9481 gaatattatt gagtaattct tgtagtttgt ttgatatttc tttatttgat attctaaata
     9541 ctttatttac tttagtttta tctattagat tgccttttga tttttgataa ccaattcctt
     9601 tacccataac gataacttct tcatcattat catctaatgt gattacaaca ttattgttta
     9661 atatcttatt aattatcatg attatcctcc ttttaagcat aaaaaaagct tatccataat
     9721 aatgggcata ctccttccta ggatactgct attattaatg aataagctta gcttgtactt
     9781 aaacaattac tacctaattc caattaatat gataaaacat cagacatatt ttgtcaactt
     9841 tttatttcca tatacttcat caatctttta tgttgctttt tgtcagttat aataattatt
     9901 cttattaata ttaacttatt tctttgcatc aatataaata caaaacaagt tatagtcaat
     9961 aataataaaa attctattaa tgtaaatagt taatattata cttcctctac tacattatct
    10021 gtactttatt taacattttt ttcaactcca aaaacatatg tcataataaa tggagcaacc
    10081 aatgctataa atataactat aagtccattt actatatttg aaatcccctt ttcccctgat
    10141 aatccaagta ctgccattat actattttgt ggaacatata aagtcacatg agtaattcct
    10201 gcaaataacc ctgcgattcc tgctcctata gatgctaaca tagcaggttt tttaagtttc
    10261 aatgtaactc cataaagagc tggttcagtt atacccataa tagctgatat ccatgcagct
    10321 gatgctaact gtttcatctt actatctttg cttcttaatg ctacagctaa tgttgcgcca
    10381 ccttgcgcta aattagcaca aacttgagat attaatatta gcgattcttg tcctgtagtt
    10441 gcaaatgctg atatgaatat aggagtcaat gcatgatgca tccctgtcat aacgataaat
    10501 ggcattgcag ctgctaataa acaaaccatt ataaatccta accttccttg tagtaaattt
    10561 attatactag ctaatccctc tcctaagaaa tttcctattg gtccaattac tattaatgct
    10621 attggcgcac ttattaataa tactaaagtt ggatttaaaa ttgtttttaa catgctaggt
    10681 gttaatttat caatgaaatt ttctatatat gacatcatcc atactgttaa aagtgcaggg
    10741 ataacagatg ctgcatatat aacaccttga attggtactc ctataaatga tatagggtcc
    10801 ccacttgata cccatgcaga gaaatttgga tgtaaaaaca cacctactac tataactgct
    10861 attgatgtat taacattaaa ttttttagct gcatttattg caagtattat aggcataaag
    10921 taaaatgcta catctcctat tatatctaat actctatatg tacttgaatt tgatgatagc
    10981 caattaaaag tggtgcaaag agataatata acttttatca tcccagatgc tgtaagggct
    11041 ggtatcatag gtgtcataca accagttatt gtatctatta tattatttaa aatactattt
    11101 tttttatttt tattttttat atcgctttta atatctcctg tggtatttat ccccttattt
    11161 attattgcat taaatacaac acctacctca ttacctatta tgacctgata ttgagttgaa
    11221 gttgagttta ctccaactac cttatctata gattttaatt tatctgcatt gactttcttt
    11281 tcatctttta agatgaacct taatcgtgtc atacaatgag ttaagtcaat tatattatct
    11341 tttcctccaa gtccatcaat tatcaattga gctacatctt catatttatt cttcatacct
    11401 tatcctctct tttaaaataa tttttaatat aattttaatc caaattaatc tttttactcc
    11461 aagcttgtaa tagtgtattg taatttaata agctagtatc ttctacaata tggtcacatt
    11521 ttcctagtac ttcttttttc cccacaccaa ttgatgtcat attggcagca tttatagctt
    11581 taatacccga ataagaatct tctattccta cacaatcata ttgttttaaa tttagcatct
    11641 tgctcccttc aataaaaata tcaggatatg gttttcccct ttttaataaa gatggattta
    11701 ctatacaatc aaattcatct tttactccca aagattctaa tatcctaggt gcattcttac
    11761 tagcagatgc tattcccaat ttcacattat tttttttaag ttcttttata agctcattta
    11821 tccctggtaa tatatcttta tatgttaatg agtttattaa ctttatataa atgttatttt
    11881 tttcttcaca cacctttaac ttttcctctt tagtataact atctacttta ttagcagata
    11941 acaatatttt atctaatgat tcttccctgt ttatgccttt taatttttca ttaaaagttt
    12001 catctatttc tatatctagc gattttgcta tctcatgcca agcttcaaaa tgatacttag
    12061 cagtatctgt tataactcca tcaagatcaa ataagactgc tttcatatta attactccct
    12121 ttaaaacaat tattaacttc tattatgttc tttgataatt ttatttcttt attatcatgt
    12181 aatatggtta tatttctacc tttgattaac ttataataag atttatattc ttctatattt
    12241 acaaaaacta aattatctct aaataatata ttaaatttaa tatttttcca gtgtttaggt
    12301 atccttggtt caaaacttaa tttatcattt tttattttca ttcctgcaaa tccatatact
    12361 aaactcatcc atgttccacc catattagca gcatgtattc catcacttgt atttttttgt
    12421 aggttattaa tatccattaa tgctgtgtcc ataaaatagt tataagcttt ttcatgattt
    12481 ccaatttcac ttgccatcat tccaaatatt gaccttgaca aagatgaatc atgagtagtt
    12541 attttttcat agtattcaaa atctctcttc ttttgttcta agctaaactc atcagggaaa
    12601 agcaacgctg aaagtacagt atctgcttgt ttatttactt gatatctata tattgttaat
    12661 ggatgataat ggagaagtaa tggatatttt tcttttggtg tatttttaaa atcccatact
    12721 gatttttcta aaaatgaatc gtcttgtttg cttattttct tctcttcatc atatggtaaa
    12781 tacatatttt gagcggcatt tatccagttt ttcacttcat attcttgtaa tttaactttt
    12841 ctaaatactt catttgaaaa taaattatgc ttaaatttat ctaccatctg tactgcatat
    12901 attaaattat acttagccat taaattagtg tagtaattat tgtttactat tgctgtatat
    12961 tcatctggtc ctgttactcc atttatacag aatttattat tttttgattc tatataatct
    13021 ccaaattcaa cccaaaatct tgatgtttct ataagaattt ctaagccatt ttgcatcata
    13081 aattcttcat cattagttgc ttcaaaatac gttctaacac cataagctat atctgcattt
    13141 atatgaaact gtgctgttcc tgctggataa tatgcactgc attcattacc atttatagtt
    13201 ctccatgaat ataagcaacc tttatttatt ccaagttctc tagctctttc tcttgatgca
    13261 tctaagattg agtatctata atctaataaa gctctagcaa tggatggttg agtatatata
    13321 aaaaatggta atatatacat ttctgtatcc cagaaataat gcccctcata gccatcacct
    13381 gtaagacctt ttgctgatat attagtcttt ccatctcttc ctgctgattg ataaatatga
    13441 aataaattgg tttttatccc cagttgaaga gttttatcgc cttcaatctc tacatcagag
    13501 gttgtccaaa actcattcat tttctttctc tgaatatttt tgactttatc aaatccaata
    13561 ttacatgctt ctcttaaaac caattctaat gattttaaat gtgcttctga ttctaaacat
    13621 tttaacgact ccagacattc taatgatttt aaacgttctt catttagatt taaatcagaa
    13681 tatactctac tatagcctac aacaatttct aaatttaacc tttcatcttt tttggcatag
    13741 acttctattt ctcgctcaat acattcatct gaaacattat aggtctcatt tgtaatattt
    13801 ccacaagtaa tatatgaatc aaacccacac cataacccta atctacttct ttctgtaact
    13861 aaattcataa aatacttatt gttatatttt tttacattaa ttttaaattg cttcttattt
    13921 atagatgcca ctcttgggtc atctactttt tcacaattaa acttctccat actattttcc
    13981 attttatttt taaattttat ttttccatta aaattcaatg gtgttattgt atatgaaatc
    14041 atcattaatt cttctatatc ttgtgatact aatctttcta tagatacttt aacttttcta
    14101 ccacgagagt tttcccatat aaatgaacgt ttaagaatcc cttcatttaa atctagtatc
    14161 ctagtatgtt cacttgtaat accttttgat aaatcaaagc aatctccttc aatagagaaa
    14221 tttactatat gagcattagg taacttacag atagtctgat tttgttttgc atagccataa
    14281 gcgctttctc catatgtaat tggagattct tcataaaatc catttacata tgtacccata
    14341 ttatctacat actcattatt aaaatcagac tcttcaatat tccctctaac tcctaaatgt
    14401 ccattagcta gtgtaaacaa cgtttcattg gattttattg tatccttact aaaccctagc
    14461 tctacaagtt ggtccttagt agtaaatatt aaactcataa taacactcct ttcttaaaat
    14521 gaattatgca tgagtttcca tatcattaaa taaaatttct ccatctgttt ctattacttt
    14581 tttataccaa aaagcacttt gttttttagt tcttttaaat gagcctcttc catagtcatc
    14641 agaatcaaca tgaattatgc catatcgttt tgtcatcttt ccttcactta cactggttac
    14701 atctatacat ccccacataa gatatcctat aagctctatt ccatcttcat ttactccaag
    14761 aatcatattt ttaatatgtt ctttcatgta atctatacga taatcatcaa ttatttttcc
    14821 atcttcattt acaatatctt gagtacccag tccattttct actataaata aaggtaactg
    14881 atacctttca tataactgat tcatagttat tcttaaccct acagggtcgt tttgccatac
    14941 actatttgat tttggaagat atggattttt tatggtttct ggtccgtttg cctttgattt
    15001 ttctaaacct tcagctctag ctgaactaca agcactagaa taatagctaa aagatacaaa
    15061 atctacagta ttatccttaa gtgttgataa atcttcctct tttattatta attttatttg
    15121 atttgatttt aaataataac tcataaagct tggatacaca cccctaactt gaacatcaga
    15181 aaaaaacaaa ttatgcctct catgctctaa agttgcaaat acatcttctg gtttacatgt
    15241 gtatggatat gtttttccag cagctaacat acacccaaac ttaaattctt tatttatata
    15301 ccttcctatt tctatagttt ttgcattagc taataattga tggtgtgctg cttgatattt
    15361 tttctgaagt tgattttcat cagctataaa agttaaccca gcacctatat atggaatatg
    15421 cattatcata ttcatttcgt taaaaggaat ccagtatttg acatcatctt tatatctttt
    15481 catcaaaatt tcagcatatt ttacatataa attaaccact cttctatcag cccacccaga
    15541 atatttttca actagagaat aaggtatatc aaagtggctt atcgttatta atggctctat
    15601 attatatttt tttaattctt ttattatatt atcataaagt tgtaatccct tttcatttgg
    15661 ttcactttct tctcctgttg ggaatattct tgaccaagat atagaaaatc taaaacattt
    15721 aaaacctatt tctgctaaca ttgctatatc ttctttgtaa ttttcacaaa atttaatccc
    15781 tatatgactt ggatagtatt gattattttc atttaaaata ttatcctcat ctaaacatac
    15841 attttttaat ctcttatcat ttaatgggag tttatcaaac tgagataaac cttttcctcc
    15901 atctaaataa aatccttcac tttgattggc tgaaattgcc cctccccata aaaaatcttt
    15961 ccctaatttc atgcatctac ctctttcaca gtataaaatt ttataatttc tatctcatat
    16021 ttagttataa gatttttttc atactatacc ttttaaataa ttcaatttaa aaatccaatt
    16081 atatttttta ctcactataa ttaatattgt ctattttcaa tactttcata ttaaatatca
    16141 atattagata tctatgtata taattatata tatagcaaat acatttttat atcctcatta
    16201 ttgttatctt attctcatat attgctgtat atttatatat ataaaaggat ttatttcgaa
    16261 atagaggttg tattgagaac taaaatttag actttataaa tgaatggaat tttcattgtt
    16321 gaaatactga aaatatattt gatttcatat aaaagaaagt gcctcatgaa ctaagttcat
    16381 tttgagacac tccctttttt ctccaagcta ttatatcttt ttttaaattc taatggagag
    16441 actccaattt tttctttaaa tactctgtaa aaagtttttg tacttccaaa accaacttct
    16501 tcaaccaaat ctacaatgga tatattgtta ttaagtaata tccactttgc tttttctaat
    16561 ctatattcat ttaaaaaagt tataaatgtt tttcctgtat tttttttgaa aaatttagta
    16621 aaataatata cactaaatcc tacttcataa gacacatctt ctaattttat aggttgttta
    16681 taattttttt ctacatattt gaatatattg ttcaatctct ctaatatctc tttagagttt
    16741 atttcatatt ctactgtatt ttcttcaatt tctctaggaa tctgtctata taatatagga
    16801 attaaattat acattttacc ttttattgca taactatatc cttctatttt gtataggtct
    16861 tcattgtaga tatctatcaa tatatcgaaa actgactttc ttatttttgc tgaccaatct
    16921 ctactacatt tttttatact actgaataaa ttaattaagc tttctttctc ctcatttaat
    16981 aaaattaaat cattaaaaaa agatatatca aattgaaata ctaatctttc acttcccggt
    17041 gatggcagta tataatgaat atctcctcct tttattatca ctatctcgcc tttttttaat
    17101 tgcataggct tgtcatttat aaatagattt actacacctt ctgttataag taatatttct
    17161 atttccttat gccagtgtgg tgttgttaat acttctcctt catttgatag agtacgaaaa
    17221 ggaaattcat gtattaattt aggcatttct aaatatattg ccatatatat cctctcctct
    17281 ctagtgtcta aaaattaatc tatactattt aaaagaatac acattttatt ttaaaaacct
    17341 caaatttata atttatacaa tacctacact tcataaaata tatctaatat ttcttttttt
    17401 gtatgtgcat ttaaaagtct ttcacctata tttttaccac ctaaaatgta agctatttta
    17461 gatattaata tcatatcttc attacctcca gattgtggca tagcaaacat aattattacc
    17521 tttacaggta agccatctat acttggccaa tctacacctt ttttagattt acctattact
    17581 atggatgcct tctttacata ttttgttttt ccatgtggca tggccatttg ataacctata
    17641 ctagtagagc ctattttttc tctatgtttt aatgccttta taaacttact ttttaaagtt
    17701 atacttcctg tatcgtataa tttatctact aattcctgta ttatgccttc ttttgtagtt
    17761 gatttcaaat ctaaaataat gctttcttct gttaatattt ctctaatttc catttgtttt
    17821 ctccaataga ttattcttaa ataatatttt aatatcctct ttgctcttgc aatctattat
    17881 atagtttata tagttctttt gcctatataa atttgatatt tccattaaaa tattctttaa
    17941 aaaactttga ttactaattt ctaaataaat atcaatcgtt ctagtaacat tattgatatt
    18001 cattatccct ttatttaatt tactttgctt atgtatatat atatttaaat cttttcctaa
    18061 tgaaatagat tcatatttac tccctaaatt cacacctatt aaactttcca tctgagctac
    18121 atcaaaatta aaatatatat tatcttctaa atacattcct aataagtttg tatctaagtt
    18181 catacttttt ttaggtttag aaagtaaagc attttttata ttattaatat cactttccat
    18241 taatagtact gatacaacta tatgtaattt atctttcaaa tctaagttta cagtagatat
    18301 tacaaagtct acatcatcta aatttcgctt actcaactga tgcataggaa ctatatcaac
    18361 tatagtccat tcaggaaatt ctctttttaa tctggctgca aggagctgag atgttccata
    18421 tcctgtatga caaactacta ttactctttt actactcata tttctttcta ttgccgcttg
    18481 aaaatatgtg gcaagatacg aaacttcatc tatactaata ttcttcagat tatcatactc
    18541 tgtcataata tttatagtca tcatacataa tcccagcaca tctgaaaatc gctcttgtat
    18601 ctctcctaac aacgggtttt ttatttggat atcatatctt aatctgttta acataggcct
    18661 tacatgagat gctagacttt tctgtagtaa gtcatcattt ttgagattta tatttaaaaa
    18721 tcgtgacata ttctctatca ttatagatga aactttttca ctttcactta atctatctga
    18781 ttcatcattg taactactat tattttcgct actaaatcca gatgatacca aaaaaatata
    18841 tatgtacata atttcatctt tcgttatttt tactctgtat ctattttcta tcttatctat
    18901 aagtgtagtg acatttttat atactaactc gtccaaatta tctacagaaa tttctctttc
    18961 ttcctgagat tctatttgat taccttcttc gattctcttt aaacatataa gtatatgggt
    19021 aattaaattt atatagtagg gctgatttat agtatatcct aaattgtatt ctaattctgt
    19081 aattatagtt tctacaaata taatagaatc aatatcaaat aaatttatca atgcactaaa
    19141 tgtagcagaa tctaatcttg ttaactcaga tatatcatgt tgttcataat tttcatctaa
    19201 taaatcatcc atcattttgg caatcgattt ccttatatcc acttctttac ctataatctt
    19261 agttccttct aaagttttat taagttttaa attgtattct tttatccatg cctctatata
    19321 tttaaaatca tttactatag aagttttact tacataatat ttatctgcta actgatttat
    19381 tgaagttata ttatttgaat ttagcaataa atactttaaa atctccattc ttctctgctc
    19441 tactgataaa gatttatttt ccttcgattg tatatttaaa ctattaatta agtctatgtt
    19501 cttctttgct aaatcttcta ttaatattcc atagcctctt tttatatcaa tttttatatt
    19561 aaaagtttct aaatatttct caatcatctt caaatctttt tgtaaagttt tatcagatac
    19621 atttaattta tctttaaagt attttatagg tctatattta ttttcgttta gtatcaattt
    19681 aagaaattca atttgcctcg acacaagcat acttagccct ccctacctat gtttatatat
    19741 ttctatgttt atattaagta ctattttaac atatttacta actatgtaaa attactaaga
    19801 tacttctgta tcataaaaga atttatgata caccttacta aattgatata atagataata
    19861 taatttcaaa ataaaaatta aggatattta gaaatagtta actgattcta aatatcctta
    19921 ttattattct atatacttgt tgttattcca ttaattattt ttactattat ttataattta
    19981 agcaccaagt aattcaactt cactattttc actccttata gtaggctttg ttacccctat
    20041 aatcaatgct gttattactg taccaatcaa tatacaagct atatatgcaa ctggtgcact
    20101 acatgcaaat ggtattacaa atattccccc atgtggtgcc attaaagtta tatctaaact
    20161 catacatatt gccccaccta tacaagaacc aattatattt gctggaatca ttcttaatgg
    20221 gtctgcagct gcaaacggta ttgcgccttc tgttatgaaa gatgccccca ttatagcagt
    20281 tggtagagat gactttcttt cttctttagt aaacttattt ttgaatatta acatagctaa
    20341 tgatattgct aatggaggta ccattcctcc taccattaat gctgctgatg gttcaaatat
    20401 attacttgaa aacatagcca atccaaaagc tgaagctgtt ttattaatag gtcctcccat
    20461 atctgatgcc atcatccctg ctaatattgc acctaataat actttattag ttccagacat
    20521 actttgtaac caagattgta aagcttcatt taaccatgat gctggcgtat taactacaaa
    20581 gaacattata gctccactcg taaatgcaat caaaagtgga catatcaata cagcatttac
    20641 tccttttaat acatctggta aattttctgt tatcttcatt actcctaaag ctatatatcc
    20701 tgttgcaaaa ccagctacca taccacctat aaaacctgaa cctccaataa ctgccataaa
    20761 accagctatc atagcaggag caaatccttg tcttcctgct attgatttag ctataaaacc
    20821 agcaagtatc ggtataaata acttaaatgc tgaatcgcct ccaagttgtc caaagaatcc
    20881 agctataggg ttgtatgttt catgagttgg gtctgatgca aatataccaa acataaaagc
    20941 tatggctata ccaattccac ctatcactac aaatggaatc atttcagata caccactcat
    21001 taagtgctta taaacaccct ttttctctga cccttgaagc atttcttttg aatcttctaa
    21061 agtctttcca cctggtttaa tttctttagc cattccattt tcagcgttta taattaaatt
    21121 tttagctaat gaaacattct tagcatctac tactttcttt tgtagcactt tctttcctat
    21181 aaatctactc agttgaactt ctttatcaca agctattatt acgtattcag ctctttttat
    21241 atcttcttta gtcaacttat tttctacacc tatagctcca tgagtttcta ttttaacttc
    21301 atatccaagt tcttgccctg cttcctctaa tgcttttttt gccataaaag tatgagctat
    21361 acctgttgga catcctgtta tcccaactat aagaccttta ttttgattat tactaaaatc
    21421 tcctgattct gcagctttat tattaaatat acttaatatt tcgtatggag tttctgcact
    21481 tagtagatgc ttaagaaaat tttcttccat tagatatgta gctagtttag atataatctt
    21541 aagatgttca tctgaacctg attctggaac tgctatcata aaaaatgacc taagtttttg
    21601 accatcatta tctgtaatct ctctatctgt tcttaaatac atcattacag gttgatttac
    21661 tacatttgac ttagcatgag gtatagctat taaattaccc atattagttg atgattcttt
    21721 ttcacgttta ttaatatcag ctataaactt ttctttatta tttatataat ttccatggta
    21781 caaatgattt gccatataat taatcacatc tttttttgtt tgtaattgtt tatctagtac
    21841 aatacaatcg cttcgtagta ttttacctat cataaattct cccctccttt taatttgtta
    21901 cctcataatt aatttttatt tttataaaag cactttaact tatctatagc attgaataga
    21961 ttcattttaa actctataac ttctcttgca gcttttatac atggtggata tatagtatct
    22021 ggctcatata agcttgggtt ttgttctaat agttctctgc acttcttata atatgccgac
    22081 ttaacatcac tcgaaatatt tattttacaa actcctagtt taactgcttg tgctatttct
    22141 tcatctggat ttgatgaacc accatgaaga actaaaggta tatctactac ttctcttatt
    22201 tcttttaata aatctagttt aagctctggt ttaaaacctt ttggatatat accatgtgct
    22261 gtaccaattg caatagctag tgtgtctact cctgtttttt caacaaaatc cttagctaat
    22321 tttgaatctg tatatattat ttcatcagta ccaccttcac ttgatgttcc tgttgttcct
    22381 attgttccaa gttctccttc tacggacaca tttaatcctt ttgctatttc tactatttcc
    22441 ctagatatag ctacattctc ttcatatggt aaatgtgagc catcaatcat tactgatgta
    22501 aatccacatc ttattgccct taatatttgt tctttatttt ccccatgatc taagtgtatt
    22561 accataggta ctttactctt atttgcttct tctatgcaac tagctacaaa gctatctcct
    22621 agaaaactta gctcatttgg gtgtattgca agtattactg gtgcattttt ttcgttagca
    22681 ctctgtacta ctgctttaag tatttgccca cttcctatat taaatgctgg tacagcaaat
    22741 tgattttctt gtgccacttt tagcatttcc ttcatgttta ttaacatatt gtacctcccg
    22801 atattttaaa ttttaggttg aatttgcttc ttgttaattt tgttgttgtt tatatttaaa
    22861 taatatcagg gtaatgaatg tcagaaaata acaatcgttt tccttagtga aaaggaactt
    22921 ttattataca ctctaagttc cttttcacta aataattttt agaagtctgc atcatagtta
    22981 attatatttc tcatgtacat cttctctttt taagtgtttt atgctttagc cttcctagat
    23041 tttataataa acaattaaga tacattaact ataaatttaa agatactcat gcaatagtta
    23101 atgctatatt agagtcaaaa aaattaaaaa gatattagct atttggccaa taccttttct
    23161 tggattccaa tagtatatac gatttataga aatcttactc acgattatct aatgttttac
    23221 ttccataaac tttacataaa acaccaaagt gacaccctcc aaattcatct ctagctaata
    23281 aagtgatttg gttttctaag caccaattta taaattagcc tttgttatct tatgtaaatc
    23341 tttttctatc taatttaaaa attattccta atgctatata gcatatacca attactaata
    23401 caccataagc catagatact cctatcttaa attcttgcat attaattaca atagaaactg
    23461 aaatagattt attacaaaca ccataaagca aggctgacat tgtatattat ccaattgtat
    23521 gaatgaatac taatatagct ccttttaatt aaatgatgct atataaatca tatttatccc
    23581 aatttttaat ctaacctatt tatatgtata ttccttttta ataaaaaaag atattgacta
    23641 aatagtcaat atcttttagt tgatttaaat acccacaatt ttaatctaat ttttaagtgt
    23701 ttccttaaca taatcttata aatattaaaa tttatctacc tataaaattt ctccattaaa
    23761 ttttattaca ttttatctta aaacaattat aatctgacct taatcatata ttatattata
    23821 taaatatttt taacaaactt aataattaaa taactatatt ttattatcca tcttataaat
    23881 gtttataaac tttccactat tcttcttcaa taaattcaaa caaattttct actgtagtat
    23941 cgaatacttt agcgatatcc atagctaact tcaaagaagg attatatttc ccattttcta
    24001 agtgtacaat agtctcacgt cttaccccaa ccaatttagc aagttcattt tgcgtcataa
    24061 ggagtttttc tctgtattct ttaatttttg ttttcatcaa tattgccact tagttacata
    24121 tccccctttt atcaaagtat atatacaaca ctaatttaga taaagactgt ataaataaaa
    24181 ctgcaatgag gattatacct gtatctaggt ttgatatatt tatattactt gtataaggtg
    24241 ttgcaaagaa aatagatatt attactatag aatatatcat tatttttatt gcaatcttat
    24301 taacctttga tagtataaat tcagatgatt catcaactaa atcattatat tttttattat
    24361 aaattgaata acatccaaaa actattaacc aactcaaccc tggaagagaa gcagtgcttg
    24421 gacctttaaa aatatactta gtggctaata cagctataaa aaaataagtt attacaagca
    24481 actccaggat aagctttgat cttacaccag tttctgattt agacatataa aatcactcct
    24541 taaaaattat attctctata ctctttaatt ttcgttttta tcaatattat cacttagttg
    24601 catattccct ttctatcaaa atgtatatac aaaattaatt tagataaaga ttgtataaat
    24661 aaaatagcta agagaattac acctatatcc aaatttgata tatccaaacc tcttgtataa
    24721 tttgctgaaa agaaaacagc tattagcaaa atagaatata acattatttt tgatgcaata
    24781 tgatcaacct ttaataacat aagttttgac gcctcgtcaa ctaaatcatt atatttttta
    24841 ttatacatga agtcacatgc ccaaaaagcg aataaaatta taaaagtaat acaaagattt
    24901 ccctctttaa aaataaactt ttttaataat gagtctataa aaaaatagga tatcataagt
    24961 aactctgaaa caagatttgt tttaacacca agctctgatt tagacatata aaatcacccc
    25021 ttaaaagtta tatatttata acaccttatg ttataaatat ataacttttc caaataaatt
    25081 gcaatagaat attgaaaata ttcaataata tcttcataaa tatattttat aaaagatttt
    25141 ataataaaca ttacaataca gtactaaatt agtttatttc atctatcatc tcaatcatat
    25201 catttactaa ctattattct aatgagcata taacattgaa acttataatt atacaaccta
    25261 aatgcctccc aatgtaccct caaaacatca tttcctgtaa ctgaagtata attggtatat
    25321 tttatcactg aaataaaaaa acactagtca ataaagacta gtattttcaa gtattttatc
    25381 attctttttt taattattgg agacgaatca tcagaataat gcccataatt tatctatatg
    25441 caaattctaa atttaaaata aagtttcaga taaatttact attctatgct atgatacaac
    25501 agaaaaaact gaaatctatg agtggtagct tcatataatc ctagttccta cagtaatcaa
    25561 aactgaggtt tattctatat gtaactaatg ataaatttta ttataggagt tattagatct
    25621 atttgtgtta ctataagtag taattatata tatgataatt ttttaacact tttctgttgg
    25681 ctatatagaa agtggtttag aatttaaatt taaataaaat ttaaattcac caaaaagaaa
    25741 tctatattag aaaccactac tctattccac cagattgtaa ttttttgttc tttatatcac
    25801 aatgctatac catattaaat tgaaaaataa gttttaaatt tatttgttac aattcattaa
    25861 ttcaaaaaaa tattttgagt catgagtttt gtaaagattt agccatgaat ttaatgcttc
    25921 tgattcaaaa acatctaaaa gtttcaatac ttgaaacgta aattccaacg gagcaattcc
    25981 tgaagccgta actaaatttt catcactaac tacagattcc gttttataat atttttctcc
    26041 agagtaattt ggtattacca ttttaagaaa ctctaagtca ttacttgtat gatttctaaa
    26101 atctaatagc ccttttttag ctaggcctaa tgtagctcca catatggctg caactataga
    26161 atcttgtctt aaagcttctt taactttttc taatattatc tcatgattca tagataacca
    26221 agtatttccc cctggcaaaa ttaataaatc cttactttcc atattaaact catcaactgt
    26281 aatttcaggt aaaactttta acccacccat tgttataata ggatttttat caattcctac
    26341 agtaattact tttagtggct ttaatccttt tttaaaatat cttcctgtat ttaattcagc
    26401 agttaaatat gccacttccc aatctgacat agtatcaaat acatacatat atacttttct
    26461 atttttcatc ttttttactc cttatgtaat taatttatat atctaattat aaaataactt
    26521 aactgacata ttcagtcagt taagctatta aatttgataa taatcaatta attctagtaa
    26581 atcttcaatt attttcttcc taaggcttag tggttcaatt acttgtattg tctttctata
    26641 aggtaaaagt atatttggta tgtacttgca tattacttcc tcttcaagca taaatattat
    26701 tttattaggt gttctttctt tcaagtagtg ccctaaaaac caatgttggc ttaaactatc
    26761 taaagaatct atactccctt ctataactaa ttgaattata ttttgttcat cttttatttc
    26821 aggtataatt ccttttataa aaaattcact tgctgaaaat tctgtcggtc tgttaaatgt
    26881 atttgatgtt tctatgattt cactaatcct atccactctg aaactacgaa cctcatttct
    26941 catattacaa aatgcaacta cgtaccaatt attattccag tgaacaattc catatggatt
    27001 tataattcta tattttgatt ttactttacg acctgcatga tagttgattt ctacagatag
    27061 ctcctttaat atactattct ccaatttttt aagtactgat tctatagaca ctttcccaat
    27121 tggttttatt acttcaaggg ctcttaagtg gtttgttaaa atatcttctt gtttttgatt
    27181 tgaatacatt tttaacttta atatcgattt atctaatgct tcgtctaaga agtatccttc
    27241 ttttttagca aatagtgctg catgaagtag agatttttgc tcttctatat caaaaaaaag
    27301 aggtgcttta gtaaaattat ttaaaaggct atacccacca ttatgacctg tatctgaaat
    27361 tataggtact ccactcgcag aaagtacatc aatatatcgg tacactgttc ttatatttat
    27421 ttctaatttt tcagctatct gttttgctgt aatttttttg tcagagttta acatccacag
    27481 tatagacatc atattgtcaa ttttagccat gtagtttcac ctctttctat ttgttatata
    27541 aagttattat ataacttttt gattgatttt ttacaaaaaa acattagtaa actgctaata
    27601 atttatacta agatttattt caaatattta tagatagtct ttttctatag ttaagttatc
    27661 cattaaatta catagtagta tatctatttt agttgatata ttttcagcta cctcccttga
    27721 atattattac aataagtcta aatcttcttt cttccataaa tatattatga ggaaagacta
    27781 tcatctttaa tggtttaata gtataaaaga tattaattta aaaaaatcta tttttatatc
    27841 agatgggtat tgttttttat aaaactgaaa aaatcaccta atagaatcta tgatttttta
    27901 acataacctc cataaattag ttccaattat cacttcaaat tcaaattttt atctgaagaa
    27961 tatacaatca tatttccata ctcaacataa tattgagtgt ttggcttgta tttacaaatt
    28021 attattctaa caaaaacaac tatacataga ttaaaatcat cttctaaaca taactactaa
    28081 aattgaatta atgatatcaa ttccattaat ctcttttaat aaaaaattct cttttctata
    28141 tgaaaattat ttcaaaactt ttaagttaaa tacaaaaatg actataaata gatttttatt
    28201 tacacaaatc tatttatagt cactatagtt tatccaataa aataataaaa taaatatgta
    28261 cacaataaat gttatttacg tcttaagtat ttatttgtta gatttttaat taaaaaaaac
    28321 aagctaatga acattaatgc taaatctagc attatattgc tttgatctcc tgtttgtaca
    28381 ttttgaccta cattactatc taaactttca tctgtatcta tatttatatc tggttttcca
    28441 ttcccatctg tatctatatt tatatccggt tttccatctc catctatatc tatatttata
    28501 tctggttttc catctccatc tgtatctata tttatatctg gttttccatc tccatctgtg
    28561 tctatattta tatctggttt tccatctcca tctgtgtcta tatttatatt cggttttcca
    28621 tccccatctg tatctatatc tggtttgcta tttcctccat tatctgggtc tggtttacta
    28681 tttccaccgt tatctggatc cggtttgcta tttcctccat tatctggatc tggtttacca
    28741 ttgccaccgt tatctggatc tggtttgcta tttcctccgt tatctggatc cggtttgcta
    28801 tttcctccat tacctgaatc cggtttgcta tttcctccat tacctgaatc tggtttgcta
    28861 tttcctccat tacctgaatc tggtttgcta tttcctccat tatctggatc tggcttattt
    28921 agatttggat tacctgtatc ataacctcct gagccagtat ctacattttt atcaggcttc
    28981 caataatcat ctactccatc attatctgta tctattgctg gacgattata atctgtatca
    29041 gggtttccat ctccattggt atctatattt ttatcaggtt tccaaatacc atctccattt
    29101 ccaccttctg tactaggctt ccattcacct gtattatcag tatctatgtt aatatctggc
    29161 ttccaatcac cactcgtatc tatattgata tctggccttc catcaccatc agtgtctata
    29221 tttatatccg gtattccatc cccatctaca tctacatttg tcatatctaa ctttaacttc
    29281 ttttcatcct cactaccttc aatgtaattt atatcatctc caatatattt tacagtaata
    29341 gtttgttcag tagtaaaaat atgatgattg ctatctaaag gtctccattc aatagatgca
    29401 gtactataat tatgactctc actaccctta ttatgagttt ttaattctac tggatcttta
    29461 tattcctttc cattaatata gaattgtacg taacctcttg gagaggcaca atctggtcct
    29521 tctttcttat ctggtgaaat agtaacattt aatgtaaacg tatctgttgg tttaaaaaca
    29581 tcagttttag ctttagactc ttctacaact aatgaagttt ctgtatcagc aggaataatt
    29641 tctgccttat aatatgctct atgcccccaa taagcatcct taaaaggata tatactcgaa
    29701 aactgtgttg atgtaatgct taattgatat ttcccagcat caacttgttt tgtaagatca
    29761 tcacttccta aatttatatc tggttccatc gtttcctcat ttaaacgttg agaactaata
    29821 gtcataaatt ttttatctgt gagtttttca cctttaggag taattattgg aataccacca
    29881 tctttaaata tccaatctaa aaatgatttt tctacgtctt cttcaaatgg taatccatca
    29941 tatcttttaa taatcaaata tggtggagta tcatcaatag taaattcttc atattgcttt
    30001 aaaaattgag aacctgaacc tatatcttta atataaaaat catctggttt tagaacttct
    30061 ccagtaattt tatctataac aactaaatta acagatactt cagatccctt aaattgatca
    30121 gagaaccata agtaaagaat cccttgagca tctgtataac taggtgctcc ataaggataa
    30181 atttttccat caattagcat tttaaaatca tcaactagtg tagtactaac tttatcttgc
    30241 cctaaatacg ttagagatat tttattcata aaaacttttg ttttcttttc taaatcaccg
    30301 tatgcaactg ctccacctaa acttttgaat gttgttgctt ttaatgaacc acctgttact
    30361 ataacatctc ccgttgtagc acttaggtca aaaatccaat ctcccactcc tgtcatataa
    30421 ggtaaaatcg ttcctccagt tacaagtata cgatatcctc tatttgttcc tggattaaca
    30481 catccatccc caaatgcact accatgtgca cctccatatg aataggaaag tccaccatta
    30541 attgtaatat tcccactata accagaacct gtgctaggat ctactactga gggacttaat
    30601 gcattctttg ttgttgtata gtatcctcca cctattccag ctccatggta tgcaccttta
    30661 gcagttataa tacctccatt gattgtgata tctccaccac taccattacc gcctccacct
    30721 attccagctc catatcctgt attacgactc gattcttgac ctatagcagt tatagcacca
    30781 ccattaataa taatatttcc tccactttca gtatttccac caccaatacc tgcagaataa
    30841 taatttccaa caacttttaa acttcctcta ttattactat ctagtagtgt aaggcgatct
    30901 ccttttttta acttgtctcc attttctaga gttacatctc taggaatttt tccaccctca
    30961 ggaactataa actctccttc aatatctcta tttacaacag aatcatcaat aattagagta
    31021 gtaccttttc ctgctctcat acctggataa tatacatcac ctgactctag cttgttttca
    31081 ctaccatctt gtaatataac atgtgcatat gcaccaccat ctttatctgg tgttaaagta
    31141 aatgggctat tagatgcagt tttaatattt actccatcaa atattaattc agcttttaca
    31201 tctttaggta taacaatcct atgtgttgtt ggagtatttg gattgttatt ttgaattgtc
    31261 atttgttttc cactaagaat agttaaaacg ccacttgcaa atttaaaatc gacatttatt
    31321 tctccaccac taattttaaa gtctcctaca gacgtaggct cttctgcagt ggcttcttct
    31381 tctacaacta ctgcattagc atctccttct gcatctcctg cattagtatc tccttctgca
    31441 tctcctgcat tagtatctcc ttctgcattt cctgcattag tatctccttc tgcatctcct
    31501 gcattagtat ctccttctgc atttcctgca ttagtatctc cttctgcatc tcctgcatta
    31561 gtatctcctt ctgcatctcc tgcattagta tctccttctg catctcctgc attagtatct
    31621 ccttctgcat ttcctgcatt agtacctcct tctgcatttc ctgcattagt actttcttct
    31681 atagtatctg cgaaagtatt tgacgcatta aatgatgcta ctggtccaag tcccattgag
    31741 atacttaaaa tcattgatag tgctttcttt ttacattttt gttgcataaa ctccccctcc
    31801 ccttaataat ttttaatata cactgtatac atccttatat acactctaat aattcttaat
    31861 atacgctgta tacattctaa tgcacactct aataattttt aatatatact taaaacttat
    31921 atttgtcaat gaatatactg ttttttcaac cttatatatt ataagtattc attatttgct
    31981 aaataactac aataaaaatt ttaattatat aaaatttatt taatttttta tataataata
    32041 ctaaggggta attaattgaa aaaactagta aaaatattaa taaatattat ttgcattatt
    32101 acgctaattt tctctagcct ttcgatttac ataaaattat ctgaatataa aaaagctgat
    32161 gaagtatata cggaactcag agaaaacact atcaataata gcaaacatca agaactttat
    32221 gataaaaata atgattatag attctggctt aaaataaaca acgtaaatat agactatcct
    32281 gtagtacaag gatataacaa tgatttctac ctaactcatg atttttataa aaattatctt
    32341 cctttaggta gtattttcat ggattataga aataattttg aaaatgataa gagtttaata
    32401 gtctatggtc attatatgaa aaataaaact atgtttggtc agctagaaaa ctatactgat
    32461 gaagcatttt tcaaagaaaa taacttagta gaaataaatt ataaagtaca aacttatact
    32521 tatgaaattt tttcagtata tactgctgac ttaattaaca gagattattt aagtatacac
    32581 tttaatagta attatgagtt taaagattat ttgaattata taacctaaaa atcagtacac
    32641 aaatctgata taaaggtaga tatttcagat aaaattataa ctttgtatac ttgtaactat
    32701 gaatttaaag atgctcgtac tatagttcat gctaaattaa tttcaaaaaa agagactgat
    32761 aagtaggttt gtgtaaatgc agacttacct acagtctttt ttgaacgtaa aattagtaag
    32821 tatggaataa tattcatatt aaacttttgt aggcaaagaa atgaaaaatc aaagagaaat
    32881 aattattaca gatttagatt accaagctga attaagaaag tgtaaaacta tggaggatgt
    32941 agttggtgaa gatagtatat aatagtttaa cttataaatt atcttggtga tattaatgaa
    33001 gtttcaaagt atatattatt tattgatata aataatatta aatctgttgc taacagatgt
    33061 acctttgttt ggagacaaac tatttaagaa atataactaa aatgttgaag tagctacctg
    33121 actttatttt gaaatgcgaa caagattttg gcattaagat aatttagaaa gaatcactgg
    33181 tacacaagga tgtgaactta gaatgaatag aagtattcag gcataaggag ctttcaccca
    33241 aataaaacat agtatgaact ttaaaagaat tctaatcaga ggcaatcaaa atgttctttc
    33301 atagtttata cctgtagtgt tagacatagt atttataagt ttcacaaata agataatcat
    33361 taataaatgt agtatgtata ttcatcctct caaaaatatt ttttaatctt acactttgta
    33421 aattattcta ttttaaaatg gtatttttat acctttattt cttatgaccg ttttcaagct
    33481 caaattttgc ataaatataa attaatgaaa agcaagtaaa aaaagaactg tcgcacagca
    33541 atttaaaatt actcattcga cagtccttat ttcgtggatt taaatagtgg agaagaatca
    33601 caagaataat acccacgatt aaacctttat acaatacatt ttatatgact ttaccatcac
    33661 taatagttat tatacaatca gccatcttag ctatttcatc attatgagtt atcataacca
    33721 acgtctgctt gtattcattt acacaatact ttagtaaatt catcacctca ttagttgttt
    33781 tactgtctaa atttccagta ggttcatctg caaaaattat tgatggctta ttggcaagtg
    33841 atcttgctat tgcaactctt tgttgttgac cacctgataa ttcattagga aatttattaa
    33901 tctgactttc taatcctaac ttagttatta aatcattaat ataattatta tctatctttc
    33961 ctttagcaat taaaatagga agaactatat tatcataaac attgataaca ggtattagat
    34021 tatagttttg gaatataaac ccaaattctt tacttctaag attagatagc ttttcatcac
    34081 ttaaagtata taaatctaaa ttatccataa atacattacc taaagttggt ttatcaagcc
    34141 ctgccataca gtgaagtaat gtgctttttc cagaaccact ttgtccaatt attgcactaa
    34201 atttatgggg ttctatctct aaagtaattc catcaacagc ctttatttca ttttctcctt
    34261 ttccatatat ttttttaata tctataactt ttaacatctt catacattta cctcccaaat
    34321 tattcattct cttttatacc ttcaataata ttcatacttt caaaatctct attacttaca
    34381 actactgata ttaaacatac aaatacagat atagcaaaga atagtaaaga ttccttaatt
    34441 gggaatgtaa aagacataaa attctctatt ccatagttta tatacgcatc attattccaa
    34501 tttgtaaatt taactagatt tataaatgat ggaatcatag ctattataaa gctaaaaact
    34561 gcatatgcta atgcttcata tataagcatt tttttcatat ttttaagact aacacctaat
    34621 gctctaagcg ttgaaatttc tttttttcta attagtagat ttgttcttat agtacagaaa
    34681 atattaattg atgctattat taaaactagt gcaattattg ctatttgtga tttaatgtaa
    34741 cttttatata tactttctga catttcatga taacttaatc tactattaaa tagagaccct
    34801 ggtatacttc ttgaaatttt ctgtatctca ttattaacaa tctcatcata tggattttct
    34861 aaatttatgc ctaattgagt gtatttttgt tctcctgtta tgtttttaga gtgaatatta
    34921 cttgttataa tttctagatc tggcataaaa tcgccatctc ccatagcaat ccattcatcc
    34981 tttaaaatta cagccactct taccttcatc tctttataaa caattgtatt attttcttca
    35041 acaggaattt ttacagttat tatgtctcct atatttaatt ctttgaatat aggctttata
    35101 gtatgatcat caactatatc ataaaaataa ttatatactg ctacatttgg atatccatca
    35161 gtgtcctttt taatatcatc taagctacct tcttcaacaa agtcatttaa cttttcatct
    35221 atataatcat catcataaag ctttaaaact aatttatctt ccattatctc ttttttttct
    35281 actccattat aggttgtaaa ttctttgttt atatccttat tattaaaagt aaacattcca
    35341 tttgttattt gaattttatg tatactttta acacccttaa ttttttctat attctctaca
    35401 tcactatctg tgtattttga tatttgcggg tctgcattta ctccaaattg cattgttata
    35461 ttatatctat tttgcatatc ttcaatttta ctgttgacct cttcttgcat acttgagaat
    35521 gtagtaatga atatatatcc tcctaaagca atagacaaca tagatatagc agctctaacc
    35581 ttatttcttc cgatattttt aagtcccata aatccataaa atccaaatat tttgcttact
    35641 agtctgtcta ttccaagtct acttctgtga tttttaattt tgtctgtatt tattattgat
    35701 tcaataggtg atatcttgcc gattttaaaa gttggtataa tacaagatat aaaaacagaa
    35761 atcattccaa ccaatatagc ttttataact atttcattat ttatatataa tttagggtta
    35821 agaccagctt cactgtataa aacttcgtta taattactaa ttcctatata tgaatatgta
    35881 accccagcta ctaacccaag tagtatacct attattgtta taattaatgc ttgatagatt
    35941 atcatacacc ttatgtttct tttctttgcg cccatcagtc taagcagtcc catttctttt
    36001 actgattgat ttagtgtaat attgaaaata ttaaatatga aaattataga tactgtagtt
    36061 attaaaacat ttcttttaaa aacattcgga tcattcttat catcttctac ataacgaagt
    36121 tcccttacaa gaggtacatt tggtagaaaa cttttttgtc ccaacttata ttttttcata
    36181 agataatcac attcaccttc aatatttctc catccacttt taaaacttaa tatactatta
    36241 taactaatcg catcatgagg tataatgtta tttgtttcat cattgccata agttactgct
    36301 tcgtattgcc aaaccacatc atagtaccct tttggttttt ccataactcc tactatttta
    36361 aattctttat ttatagaaaa taattgatta cttccgttag tatctttata gttctttttt
    36421 attgttaaat ttaatgtaga tcctactcct tcacttaaat tcattgcttc tagtgctttt
    36481 ttctctaata ctatttcatt gttgttttta ggtagtctac cttctattat tttgttgtat
    36541 ctacttttta gatactcttc gtttgatgaa gttaatataa atgagtttcc ttttttatct
    36601 gaaatacgtc ctagattttg taccgaatta ccctttgata ctttttcatc agtttctaat
    36661 tttttagcat catcaagtgt caaatcacta aactctacat ggtatccact gtaaagttca
    36721 tgtattgcta ctacattgtt ttcattttgt gaatctttta taacactagg tccaaatatc
    36781 aacataacac ctaacataac tcctattatt aaaagtaatg ttctaagttt atttttcttt
    36841 aaatatgcta gtgacatttt taagtaaatc cccatataat cctcctattt tactataaat
    36901 tatttatcca actcatctgt taagtagatt ggtggatatt ttaaatcttt taattcttca
    36961 ttattttttg aagcttacta ccatcttcct catgataaac ttgcagtatc accttattta
    37021 catgtagtgc ataataatca ccattataaa aatattagta tttataacct ggataatttt
    37081 tgtaaaatga tttataaagt atcccatcct aattctattt tgtatacttt tcttttgtaa
    37141 tacgctctat atcatcacct gaaactcctt gatatgtcat atatttatct atatattttt
    37201 cagcttcatt ccattttcta aaatagttgt tgtatataat aaatcctatt atggctataa
    37261 tgcttataat taaagttaat aaaatagctt tgtattttct catagtgaat cttcctactt
    37321 caatttaaat tctttaataa gttttaattc taaagactta gttttatcat caaatttctt
    37381 aacttgaatt tttatatctc ccaaattatc aacctctaca tttacttcac ctttatcaaa
    37441 tacttcttca atttttctaa aaaattcttt ctcacttatt tctatatgaa tcaatgatct
    37501 atatgttata aatggccatt atgaatttat catcttctga cagcttttca ttataagaaa
    37561 aatctttaac acattgaatt gataatattt ccttcgtatc cttcttttcc ttagttattg
    37621 aataatgtaa aacttggcta ggttgttctt ttgtatactc caagtttccc aaagacaaaa
    37681 caaaacttcc atctgattta ttttcagcta ccctaagacc actattttct attgattctg
    37741 atattttctt tgacaagtct tcttgatact agaaatctgt tcaatagtat atattttttc
    37801 atttgtacct gaaaccttat ttgtgcatcc tgttattaaa cctagtgtta agcttatagc
    37861 tataaccata gttattgtaa tttttttatc ttcatataat cctccttata tattgaaaac
    37921 tcgttatttc tcataaaaaa aagacgctat ctttttctat taaaagataa catccctata
    37981 tgtttagtat caatatatgt tccccaactg gtaggttaat cgacccaact atcaaaattt
    38041 tgatttaatg gtatataaat attcaatata aatttgtctt ttttatcttc aaatgataaa
    38101 tctcctctat atttttttat agaagatttg atgctttaaa tccctattcc atgtacaagt
    38161 ttatcttttt tatctgttaa taataaactt ttattctttc taattgaatt tattttacta
    38221 ttctcacatt taattacgaa ataggacttt gagatagttc cttttatttg tatatattta
    38281 tcaatacttt tatcatgtat cttatcgcaa gcttctattg cattatctaa gtatttgcaa
    38341 atatattaga tacatctatt ggttttataa aattcacttt ggaaaaattg atatcacaaa
    38401 tgaatttaat ttttcttttt tcacttacat cacttttttc atttattatt atatccaata
    38461 taacattacc tgtattgtaa attgatttaa attctttaat ttaatctttt aaactattta
    38521 catacccatc aattttcctt ttttatattt gagattttct atacaatata tatgattatt
    38581 tatatcatga taaagcttct taactttagc gtgtgattct tgaatactta gataatgaga
    38641 atactgcata tctaacttat tttttattac ttcattttgc aattttaact tattttcctt
    38701 aattattttt ataaacacta taataatgac taagctacta gctagtatta acctaggtat
    38761 cacaatatac ccaactatat atatttccta atacccacca aatcgataca tttttctatt
    38821 tatataaatt attaatatag taaataaatt tgttattaaa gcaacagata agtatatata
    38881 gtttatttta ttatctttat ctagtcccaa tcttttatta ataaatttaa atatagcaca
    38941 agctactata taactattat tataagataa tagtatatat ctactttagc attatcataa
    39001 ctaaacacta atagagcttc ctctgatgat attacattac ttgtaactac aaaaaataaa
    39061 tttatagata taacatcttt tattaaaggg tgatatacgt caaataagaa aaatgaaact
    39121 atatatttaa caattcctat cttataaaaa taattatagt aagttcccaa gtaataaata
    39181 tcaaataata gattatatca tatagatttt tcttcatatt ataatcatgg gtcttatcaa
    39241 cattaaataa tataatgatc ttagttataa gataagctaa taatacagta aaaaaagata
    39301 ttttcttagt cttatctgta tctttatcgt aagttaatat actttttagt atcattattg
    39361 gtgtaaacaa tgataatatt aataatattt tatttatatg gtttaacata ttctatcacc
    39421 aataaatttt agaaacccct cttttatatc tttatatcta tatctactaa taggtacttc
    39481 ttcttgactg tctaatattg cagtatttga ctttatattt tctacatagt ttaagttagt
    39541 tatataactt ttatgacatc ttacaagttc ctctatattt aaatcttttt ctattttttc
    39601 taggatacat atagcatcaa gagttttatt ttttgtatgt attagcatgt tctttttttg
    39661 aacttctata tatttttttg ctagattaga ttttgtatgt gttagattta tttttatttt
    39721 tttcaatttt tttggtacat gttaatatgt gtttttctag ttcttgaaaa tttattggct
    39781 ttaataagta tcaataagct cttacttcat atctttcctg tacataattt attagagatg
    39841 ttataaatat tattatctac ttttcttatg tttcttgcga tatctatact atttagttct
    39901 gccattttta tatctaagag tagtgtatcc tctcaattaa ctttgtaatg attcttcttt
    39961 tgagctatat ttttttaatt cgatattctt tgtttttatt ttttaatata ttatcaactt
    40021 gattttttat tacgatttgt atttcttttt catcatcaca agctgttatt gtaatgataa
    40081 cctctcctaa tattgtattt ttatttagat tcttcacttt aggttaaaac tttttaaatg
    40141 tatttatcaa taatatttaa attaaaaatg tataaaaata cctatatcca attggatata
    40201 ggtattcgtg gatttcaatg gtggagacga ggggagtcta taagtggtta tttgattgtt
    40261 ttgttgtttt ttgcaagtgt aggtatatag ctatttagtt atgatgttaa actgattttt
    40321 tcggatattt tttattgttt atttttgtag ttggggtatt attggggg
//
LOCUS       DAMJYR010000027        37834 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.027, whole genome shotgun sequence.
ACCESSION   DAMJYR010000027 DAMJYR010000000
VERSION     DAMJYR010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 37834)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37834)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37834
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.027"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            77..1771
                     /locus_tag="PC471_001323"
     CDS             77..1771
                     /locus_tag="PC471_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HDF2788459.1"
                     /translation="MKFSEFKYERPNYNSMKKEFLSCVEDINNSRNYKEQQKNIHKIN
                     LLRNKIETLSNIASIRYSTDTFNKFYKEEKNYWDEYMPLYEELNSYFYNAIVNSKFKY
                     DLIKEFGEQFFTIVEYSLKSFSKEIISELQEENKLCSEYTRLLASAEIMFDGKIRNLS
                     GMGKFMYSKNRKTRELANKAYYNFFEENETKFDDIFDKLVKLRDKMSKKLGFEDFVEL
                     GYIRMMRSDYREYMIKNVRKQVLKYVVPMANELYEKQAKRIGLEYLSYIDEGVEFLTG
                     NASLKGDSRYIIKNGKRMYSELSKETNEFFDFMLENELMDLETKKGKGAGGYCTYIPD
                     YKSPFIFSNFNQTADDIDVLTHEAGHAFQLYMSRWIDMPEINFPTLDSCEIHSMSMEF
                     ITWPWMDLFFKEDTDKYKFTHLSSSIKFIPYGVIVDEFQHYIYKNPNVDKSKRKEIWR
                     FLEKKYLPHRKYGDNSFLERGCWWFKQGHIFKNPFYYIDYVLAQICALQFWKKMIQDR
                     DAGWKDYINICKVGGTKSFLDIVSMGNLYSPFDDGCIESIIGDVKSWFDEINDSKL"
     regulatory      2103..2213
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            2317..2688
                     /locus_tag="PC471_001324"
     CDS             2317..2688
                     /locus_tag="PC471_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrdX family protein"
                     /protein_id="HDF2788460.1"
                     /translation="MIIITNNPKVKEEVQGREVLFKDTTYIGILEASRDLIHEGYELL
                     SHPLYGSVKPNETPYRTVILKKGNRLDINSLTLIEEAIITASKFQNNKKTPKWTESVQ
                     DDFRVIDYDIFYNTIQRMQYE"
     gene            2783..3730
                     /gene="trxB"
                     /locus_tag="PC471_001325"
     CDS             2783..3730
                     /gene="trxB"
                     /locus_tag="PC471_001325"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HDF2788461.1"
                     /translation="MENVYDLVIIGSGPAGLAAGLYGARAKLKTLILEKDKTGGQIVI
                     THEIANYPGSVPNATGPSLIARMVEQCKEFGAEMLRDNIVDTELDGDIKVLKGEKAEY
                     RAKAVIIGTGATPRKIGCPGEKELTGKGVSYCATCDADFFEDFEVFVVGGGDSALEEA
                     MYLTKFARKVTIVHRRQGFRCAKSVEEKAKANPKIEFLLDTVIEEIKGDGILESVVFK
                     NKVTGETHEYFADEEDGTMGVFVFVGLDAQTDLFKGKVDMDEKGYIITDEDMRTNIPG
                     VFAAGDCRSKTLRQVVTATNDGAIASIVAEKYIDEKFGN"
     gene            3834..4151
                     /locus_tag="PC471_001326"
     CDS             3834..4151
                     /locus_tag="PC471_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol reductase thioredoxin"
                     /protein_id="HDF2788462.1"
                     /translation="MLDLDKATFEEEVLNAEGFVFVDFWSEGCEPCKALMPDVHKLAE
                     TYGDKIKFCKMDTTKARRLAIKQKVLGLPTMAIYKDGEKVDEVTKDDATVPNIENMIK
                     KYL"
     gene            4420..5706
                     /locus_tag="PC471_001327"
     CDS             4420..5706
                     /locus_tag="PC471_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006439082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase component B
                     subunit"
                     /protein_id="HDF2788463.1"
                     /translation="MRLELGKIFIKDIQFGDVTEVKDGVLFINKQEMLQEIGGDEHIK
                     SIDIELARPGESVRITPVKDVIEPRVKVEGNGGIFPGIMSKVDTVGEGKTHALKGVAV
                     VTTGKIVGFQEGIIDMTGEGAKYTPFSKLNNVVVIAEPIDGLKQYAHEKAVRMIGFKA
                     AMYLGEVARNLTPDEVSVYETKPLLESIKEYPELPKVGYVYMLQTQGLLHDTYVYGVD
                     AKQIVPTLLYPTELMDGAIVSGNCVSACDKNPSYVHINNGVVEDLYARHGKDINFVGV
                     IITNENVYLADKERSSNWTSKLCKYLGLDAVIVSQEGFGNPDTDLIMNCKKIEMQGVK
                     TVIVTDEYAGRDGGSQSLADADVRADAVVTGGNANQVVVLPKLDKVIGHLEVVDVIAG
                     GSDGSLRADGTIEVEIQAITGATNETGFGHLTAKGY"
     gene            5818..6291
                     /locus_tag="PC471_001328"
     CDS             5818..6291
                     /locus_tag="PC471_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011986236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:5950..5952,aa:Sec)
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase complex
                     selenoprotein A"
                     /protein_id="HDF2788464.1"
                     /translation="MSLLSNKKVLIIGDRDGIPGPAIEECVKTVEGAEVVFSSTECFV
                     UTAAGAMDLENQNRVKDAADKFGAENVVILLGAAEAEAAGLAAETVTAGDPTFAGPLA
                     GVALGLSVYHVVEEPIKSLFDESVYEDQISMMEMVLEVEEIEEEMSGIREEFCKF"
     gene            6323..7633
                     /gene="grdB"
                     /locus_tag="PC471_001329"
     CDS             6323..7633
                     /gene="grdB"
                     /locus_tag="PC471_001329"
                     /EC_number="1.21.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:7370..7372,aa:Sec)
                     /transl_table=11
                     /product="glycine reductase complex selenoprotein B"
                     /protein_id="HDF2788465.1"
                     /translation="MGKLKAVHYINQFFAGIGGEEKADTKPHVAETLPPISLQLDKLL
                     GEDIEIVGTVVCGDSYFNENIDSASEEVLSMVKGFEPQLFIAGPAFNAGRYGVAAGTI
                     TKVVKDALNIPALTGMYVENPGADMFKKDVYVVETSDSAAGMRKALPKIAKLAVKLAN
                     GEEIGTPKDEGYIARGIRVNYFHEDRGSKRAVDMLVKKIKGEPFETEYPMPNFDRVDP
                     SKAVKDLSKCKIALVTSGGIVPKGNPDRIESSSASKYGTYSIAGVMDLTEETYETAHG
                     GYDPVYANLDADRVLPVDVLRDLEKEGVIGKLHETFYTTVGNGTSVANSKKYASEIGA
                     ALVADGVDAVILTSTUGTCTRCGATMVKEIEKTGLPVVHMCTVVPISLTVGANRIVPT
                     IAIPHPLGNPALDPTEEKALRRGLVEKALNALTTEVDGQTVFEK"
     gene            7889..9421
                     /locus_tag="PC471_001330"
     CDS             7889..9421
                     /locus_tag="PC471_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014520415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III family protein"
                     /protein_id="HDF2788466.1"
                     /translation="MTYPVLKGAGYVLIHTPDMIVQNGSTCTVERATNPDSEFLKEVS
                     NHIRSYEDVVNYMPNQVYIGNRRPEELRDLPMPWCEQKIEGTRNGKFGEIMPQDEFIA
                     LMQISDAFDLVKLSQEFIDEVKPKIENNYPEIAPFVGKLKGDDIEEGKELVATHIAEG
                     LYHDGKFVGYVKRAHDVDVNLNAHTMFENLVVKASGVLSAIQMLRHSKIDPAEIDYVI
                     ECSEEACGDINQRGGGNFAKSIAEIAGLQNATGSDTRGFCAAPTHALIQAAALVKAGI
                     HKNVMVVAGGASAKLGMNAKDHVKKGLPVLEDVVGGFAVLVSENDGVNPVIRTDLTGK
                     HTVGTGSSPQAVMTALITSGLDRANLKITDVDVYSVEMQNPDITKPAGAGDVPEANYK
                     MIGALAVKRGDLEKKELKDFVSNKGLPGWAPTQGHIPSGAPYIGFLIDDLTTGNRNRA
                     MIVGKGSLFLGRMTNLFDGVSFIAERNTGVTEETSGISKDEIKKIIAESMKKLALDML
                     EE"
     gene            9432..10577
                     /locus_tag="PC471_001331"
     CDS             9432..10577
                     /locus_tag="PC471_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine reductase"
                     /protein_id="HDF2788467.1"
                     /translation="MSKKVIADVFLEVANAIESGEFGKKVKIGVTTLGSEHGVENMVN
                     GAQLAKSNLFDIVLIGPKVETDLEVVEVNDEKEMHAKMEELLDSGYIDACVTMHYNFP
                     IGVSTVGRVITPAKGKEMILATTTGTSATNRIEAMVRNAIYGIATAKSMGNKCPKVGI
                     LNVDGARQVEKCLKELKDNGYDMEFADSIRADGGCVMRGNDLLVGAPDVMVTDTLSGN
                     IFMKVFSSYTTGGDYEAQGFGYGPGVGEDYDRKVLIVSRASGSPVVANALKYAYDVVK
                     GDISNVARNEFAKVKKAKFDDIISSLTKKEVKAEKVEVKMPDKEIVTRQIAGVDIMDL
                     EDAVSELWKNGIYAESGMGCTGPIVLVNEAKGDLAVETLVKAGYTAK"
     gene            10887..11966
                     /locus_tag="PC471_001332"
     CDS             10887..11966
                     /locus_tag="PC471_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HDF2788468.1"
                     /translation="MSRVKNVVELLETKGVDALYLTKKTNVNYISGFPDEEAYAVICK
                     DGNFLVTDSRYMELAEKVCKDFEIINWHNFDRSVAKAVKSVCDKVGIKKLGFERTNIV
                     FDKYEELKNLIEKDNGELIPTENIVETLRYVKDKDEIKNTRKACEIADKALEELIPHI
                     KAGVSEIELATKLEYFMKMNGAQNIGFETILISGAKTSLLHGKPSDKIIEKGDFVLID
                     YGAMYNGYISDTTRTFIVGGASEKQLEIYNLVKEAQNVGVENMKAGVHATIPDAEIRK
                     VVKKYEDYYYQGIGHGVGRDVHEEPFIGNYGDKIIEEGCIITMEPGIYFPGWGGVRIE
                     DTVLITKNGPERLTKFPKDLMILDK"
     gene            12079..12678
                     /locus_tag="PC471_001333"
     CDS             12079..12678
                     /locus_tag="PC471_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2788469.1"
                     /translation="MYKKHKFIKNIKKAWYKIKLIDRCLIIVMAILMFQSIYNLFVNE
                     VNSQDTTTIDVVVRTTSAAIFGYFLSANFIKRPLRKYTNTDVSSNLFIDNNLSPKGQE
                     SNQNNIMNVKNTIGFTSEADNYEKKIPINNNEGFEEGETSELQIIIATVICIVALTVL
                     FVVRNFTTTTSASLGTISQMRDFVSGCVGFLLGCPSKPD"
     gene            complement(12864..13736)
                     /locus_tag="PC471_001334"
     CDS             complement(12864..13736)
                     /locus_tag="PC471_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDF2788470.1"
                     /translation="MNINYLYYFQTVCKYKNMTKAAESIHISQPSITLAIKELEKELG
                     FELFYRIGNKIELTPEGKIFLDKSKHFIKQFEDFQCDALDLGKKRKASLKIGIPTVLG
                     TFLLSKILPRFNVIYPDIELKIFEVPTFVGAKMIEESTLDFCIGIIDSDIYDDIDSKT
                     IYKTELYLVTNPKNELAKHPIISNYMLKNVPFVILSEGSYHYKIITKRLEKAKPNIIL
                     HSNQLSTIRYLLENDLASTILYKEIFQNTENLCSIPLERAITANIGVLWRRNQYISHS
                     MKLFIEYMASIHIN"
     gene            14153..15556
                     /locus_tag="PC471_001335"
     CDS             14153..15556
                     /locus_tag="PC471_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2788471.1"
                     /translation="MKGSDLVKFELMDFLMNPFVLMFAAVITGILFGKIKFGKFNFGV
                     SGALFTGLFIGWLAYSLGNLIIAKGETAAGYKAATVMMGNGIISSDFFDFFLIIFVAA
                     VGLLAAKDMKAVLKKYGARFVILGVLITFIGGSMTYAMTLLSSDKGSSAYEVSGVYTG
                     ALTSSPGLAAALETAGKHAEDVSKEFEKASIKDKKEILKVVDPEGKLDVNTTTSLTQE
                     QIDKYIAYAEAGVGIGHAVAYPFGVLIVILGVNFLPKLFRMDLKEERRKYEKEMKEAR
                     DSVSGKTIPEVPFNIITFFLTCLAGYLVGGIHVFMGPLGYFTLGATGGSLIVSLVLGY
                     IGKIGVVNFRMEEKVLNILKQIGLVFFLAIVGLRYGGKVVDSIMTSGMHLALVAIAVG
                     VTAMMIGFLVGKYVFKLNWILLSGAVCGGMTSTPGLGAAVDALDSDDPAAGYGATYPF
                     ALLTKVILVIVLHKLPM"
     gene            15583..17127
                     /locus_tag="PC471_001336"
     CDS             15583..17127
                     /locus_tag="PC471_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase family protein"
                     /protein_id="HDF2788472.1"
                     /translation="MNNEGAFEELSGRLRNKELCDLIMNADEAAKIIEDGMVIGVSGF
                     TPSGYPKAVPLAVSERAKSGEKIKLTVYSGASLGPEVDGAWSEAGIIERRLPYQTNSI
                     LRNNINKGVVDYIDMHLSHSTQFLNYGTIPKVDVAIVEALAITEEGNIIPTSGIGNSP
                     SFIKSADKVIVEINLAKPMEMEGMADIYITENPPNRKPIEINHPKDRIGTTYIPCGLD
                     KIAGVVITNMQDKTRPLGVVDEASKKISNNIIAFLREEVKAGRLSKNLLPLQSGVGSV
                     ANAVLYGLCESEFENLTCYTEVVQDSMLDLIRMGKVTMASTTSVSPSPEGLIKFEKDI
                     DFFKDKIILRPQEISNNPEIARRIGVIAMNTAIEVDIYGNVNSTHIMGSKMMNGIGGS
                     GDFARNGAITIFSTESIAKNGDISSIVPMVSHVDHTEHDVMVIVTEQGYADLRGLAPR
                     ERAIKIIENCAHPDYKEQLRDYLNRACKSGAKQTPHILDEALSWHSKFMSTGTMKKAE
                     TFKSAL"
     gene            17158..18549
                     /locus_tag="PC471_001337"
     CDS             17158..18549
                     /locus_tag="PC471_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HDF2788473.1"
                     /translation="MEKAVENFEDLSKEYINGYIERARKAQREFECYTQEQVDKIVKI
                     VGKVVYYNAEYLAKLAVEETGMGVYEDKVAKNKSKAKVIYNNLKDKKSVGIIDIDRET
                     GITKVAKPVGVVAAITPCTNPIVTPMSNAMFALKGRNAIIITPHHKAIGCSTKTVEMI
                     NEELEKIGAPENLIQILDQQSRENTRNLISSADVVIATGGMGMVKAAYSSGKPALGVG
                     AGNVQCIIDRDVDIKEAVPKIIAGRIFDNGIICSGEQSVIVAEEMFDKIMDEFKNNKG
                     FIVRDKVQKEAFRNAMFVNKSMNKDAVGQSVHTIAKIAGVEIPEDTKIIVIEADGPGE
                     EDIIAKEKMCPVISAYKYKSFEEGVAIAKANLNVEGKGHSVSIHSNTVKNIEYAGENI
                     EVSRFVINQCCATSAGGSFFNGLAPTNTLGCGSWGNNSISENLDYKHLINISRIAYYM
                     PENEVPTDEELWG"
     gene            18742..20238
                     /gene="abfD"
                     /locus_tag="PC471_001338"
     CDS             18742..20238
                     /gene="abfD"
                     /locus_tag="PC471_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybutyryl-CoA
                     dehydratase/vinylacetyl-CoA-Delta-isomerase"
                     /protein_id="HDF2788474.1"
                     /translation="MALMTGAQYIESLRKLNTKVYMFGEEVKNWVDHPMIRPSINCVA
                     ATYDLAHDPEYADLMTVTSNITGEKINRFGHLHQSVDDLIKKVKMQRLCGQKTASCFQ
                     RCVGMDAFNAVYSTTFECDKAHGTNYHDNFVKYLTYIQENDLVVDGAMTDPKGDRSLS
                     PSAQPDPDMFLHIVERREDGIIVRGAKAHQTGSINSHEHLIMPTISMTEADKDYAVSF
                     AVPSDAEGVFMIYGRQSCDTRKLEEGADVDLGNKEFGGQEALVVFDNVFVPNDRIFLC
                     GEWDFSGMLVERFAGYHRQSYGGCKVGVGDVIIGAAALAADYNGANKASHIKDKLIEM
                     THLNESLYCCGIACSSEGHKTEAGNYQIDLLLANVCKQNVTRFPYEIVRLAEDIAGGL
                     MVTMPSEKDFKSDLKVGTSGMTIGEVCNKYFKASSVASTEERMRILRFLENICLGSSA
                     VGYRTESMHGAGSPQAQRIMISRQGNINQKKELAKKIAGIKKEEALNL"
     gene            20373..20648
                     /locus_tag="PC471_001339"
     CDS             20373..20648
                     /locus_tag="PC471_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2788475.1"
                     /translation="MTCGVRFCGGCNPRYERGKALTIIKEHFNGRVDFSIAQENVEYD
                     LLLIIGGCATLCASYQQYKVKNECVKMWDFSHIERVINVIESAICET"
     gene            20672..21979
                     /locus_tag="PC471_001340"
     CDS             20672..21979
                     /locus_tag="PC471_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase family protein"
                     /protein_id="HDF2788476.1"
                     /translation="MSWQELYQSKLCSATEAVKQIKNGDTVVFAHCVGEPPALVEAMI
                     ENAEQYKDVEIKHMVSLGSGGYTAKGMEAHFRVNPMFVSGNVRKAIENGDGDFTPAFF
                     HEVPKLLREKRLKCDVVLAQVTPPDEHGYCSLGTSVDYTYEAIKTARTVIVQVNDQFP
                     RTYGEVVHVSEFDYIVEKSQPLFELQPAKIGEVEEAIGKNCASLIEDGSTLQLGIGGI
                     PDAVMLFLTDKKDLGIHSEMISDGTLALYEKGVINGKYKNFDKEKMTVTFLMGTKKLY
                     DFANNNPAVEVKPVDYVNHPAIIMKQHKMVSINSAIQVDLMGQVVAEAMGLRQFSGVG
                     GQVDFIRGVSMGEDGKAIIAMPSITTKKDGTVISKIVSIVDEGAPITTSRNDVDYIVT
                     EYGIAELKGKSLRERARNLINIAHPSVREHLAVEFEKRFKEKY"
     gene            21999..23117
                     /locus_tag="PC471_001341"
     CDS             21999..23117
                     /locus_tag="PC471_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybutyrate dehydrogenase"
                     /protein_id="HDF2788477.1"
                     /translation="MEALRVVPKIFYFDTFKEFNEEFKIGKNDLVITNEFIYEPYMKP
                     LGIDTNLIFQEKFGTGEPSDEMIDSMTKEMKKYNFDRIIAFGGGTIVDICKILALDVP
                     EKSIDLFEGEVSPKKVKELVVVPTTCGTGSEVTNVAIAELKSKHTKKGLAVEETYADY
                     AVLIPETVKGLPYKFFVTSSVDALIHAIESYLSPKASPFTEMYSLQAIKMIMDGYKKI
                     VDKGEEERFNHLRDFVLASNYAGIAFGNAGCAAVHALSYSIGGAFHVAHGEANYQFFT
                     EVFKMYSRKKPNGKIIKCTKILADALECDPNCDVYGELEKFLNKLIAKKALREYGMVE
                     SQIDEFTDSTIANQQRLLANNYVELSREEIREIFANLY"
     gene            23404..24324
                     /locus_tag="PC471_001342"
     CDS             23404..24324
                     /locus_tag="PC471_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     family protein"
                     /protein_id="HDF2788478.1"
                     /translation="MYKSENFSKKSVLINSFLSKFLKIIGWGLVSLFTLSALVLSTIS
                     IIIGWAVILPIQVCAVFLLIGGVCIFKGKRLGDQISRYKKYCTIINNRNIIPVELIAE
                     NTSKSLNFIIKDVQKMIDKNYFINTYIDKRNNQIVLTNEDFVPPRYEDVAYEVKEEEK
                     TDESNEVDTIINKGMNYLTQIKEANKNIKSESMGDKIVQVEDVTSKIFDVVKHDPSKL
                     TQIQKFMDYYLPTTLKLLNSYHTLEEQGIDRENITTTMESIENTMNTIVVAFENQLDY
                     LFEDEAIDISTDITVLENMLVQEGLTSGKF"
     gene            24439..25596
                     /locus_tag="PC471_001343"
     CDS             24439..25596
                     /locus_tag="PC471_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HDF2788479.1"
                     /translation="MNNLDDIPVMPTLTLDPFGEESNTNDIDNSDLLMKKDEKDPEEE
                     KLSESERKMVKEFAEKIDITNTNMILQYGAGAQKKVASFSETALKSVKTRDLGEVGDM
                     LTNLVTDLKAFSADEKEQSGFLGIFKKANNKISNLKTKYDSAEVNVDKVSKELQKHQV
                     KILKDIAMLDKMYELNLAYSKELTMYIIAGKQKLKDMKEYEMPKLREKARLSGSTEDA
                     QSVNDMVSLCDRFEKKIHDLELTRMVSLQMAPQIRLVQNSNNLMAEKIQSTIVNTIPL
                     WKNQIVLALGISHLNQAMKAQREVSNMTNELLLKNAETLKMGTIETAKESERGIVDID
                     TIKKTNQSLISTIDEVVKIQHDGRIKRQEAEVELSKIENELKSKLLEFTVK"
     gene            complement(25681..27180)
                     /locus_tag="PC471_001344"
     CDS             complement(25681..27180)
                     /locus_tag="PC471_001344"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HDF2788480.1"
                     /translation="MAFYFDTPSHTFSEYLLVPGFSSTDCIPANVSLKTPVTKFKKGE
                     EADIYMNIPLTSAIMQSVSDDKMAIALAKEGGISFIYGSQTIENEAAMVARVKSHKAG
                     FVVSDSNIKPDNTLKDILDLKEKTGHSTVAVTEDGTSTGKLLGIVASRDYRISRMDLD
                     TKVSEFMTPMSSIVYANKDVTLKEANNIIWDHKLNSLPVLDDNGNLMYMVFRKDYSSH
                     KENPLELLDSSKRYVVGAGINTRDFAERVPALVEAGADVLCIDSSEGFSEWQKITLDF
                     IREKYGDTVKVGAGNVVDREGFLYLAEAGADFVKVGIGGGSICITREQKGIGRGQATS
                     IIEVAQARDEYFEKTGIYIPICSDGGIVYDHHITLALAMGADFIMLGRYFSRFDESPT
                     NKVNINGSYMKEYWGEGSARARNWQRYDLGGDKKLSFEEGVDSYVPYAGSLKDNVTLS
                     LSKVRSTMCNCGALSIPELQKNAKLTLVSSTSIVEGGAHDVMLKDNRNL"
     gene            27776..29200
                     /locus_tag="PC471_001345"
     CDS             27776..29200
                     /locus_tag="PC471_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICB"
                     /protein_id="HDF2788481.1"
                     /translation="MSSSNQSTIKTSIQRVGKFLSGMVMPNIGAFIAWGLITALFIPT
                     GWMPNEKLSTIGDPMIKYLLPLLIAYTGGKAIAGQRGGVIGAAAAMGVIVGADIPMFI
                     GAMIMGPFAGWVIKKFDKFVDGKIPTGFEMLVNNFSIGIIGMLLAILGFYAIGPAIVA
                     GTALIESGVQFIVSKSLLPLVSVFIEPGKVLFLNNAINHGILGPIGIAEAKEAGKSIM
                     FLLESNPGPGLGVLLAYWMFSRGSVKQSAPGAVIIHFFGGIHEIYFPYILMNPVLILA
                     TMAGGAAGILTFSILGAGLVAAPSPGSIFALMALAPKGGLLPVLAGVAVATVVSFLVA
                     APFVKRASANQSEEDSTSLEEAKAKMSDMKSASKNSEKNIEEKQLEVNEIKKIVFACD
                     AGMGSSAMGASRFKNRIKNLDLNIEITNSSVDNLPDDTQIVVTHNTLVERVAKNNSSV
                     EIVSINNFLNDPNLDTLFKRLESK"
     gene            29235..31364
                     /locus_tag="PC471_001346"
     CDS             29235..31364
                     /locus_tag="PC471_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HDF2788482.1"
                     /translation="MKKKKITSRQKKIILMIVANSKKNIPITISEIAGTLELSSRTVL
                     RDMSGIEKWFDENDFNFVKKPGVGLILEENIENQNFIIELLEEEKIEKEYSKEERNLI
                     ILSKLLVSNEPVKSYYFAKILKVSEWVLNNDFALASKWLERFDIELVRKPGLGVYLKG
                     QEKNFREAYVNLIYDSFNEKEILDMVRNISENIQTDKAIEILSENRLLNLMDRCIIRK
                     VEATLTKKLSDLDVNLADSAYIGLVVHISLALQRIKNGENITMDKEFLKELSITEEFK
                     LAKEIVKGMETDFNMDIPVDEVGYITMHIRGAKQRSSSNHKALNLDDIEIMEITNKMI
                     DLAEDEFKISLKNDERLFKDLANHLGPSINRLNMGLEIRNPLLDEIKSKYSYAYDVVE
                     KISRIIKDKLNINSIPESEIGYIAMHFASAIEKNLMMNTNINIVVACPTGIGTSRFLS
                     TKIENKFPNLNILETISAINIDEEYLKEKDVDLIVSTVELNTSLNYICVGPFMSLDDE
                     QIIKEKIKSIAQNKLINLNAKNDTKSKNKVYEQITESMNIGKDILQFLEEIRFEKFES
                     KDLSELIEDSSKIFAKSSEDIISIKESLKERLKISIPYIEESKILLLHCMSERIDIMK
                     LSIIKLENRIVLDSNEEIDNVVFMLLPKNSPSYQRQIMSEISGSLIDNIIFTNKINKF
                     SIEEMALEVKDIVFNFYTNRLKAFIDK"
     gene            31394..31816
                     /locus_tag="PC471_001347"
     CDS             31394..31816
                     /locus_tag="PC471_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDF2788483.1"
                     /translation="MSKVLNENNIFLGLDSVSKEEAITLAGRKLVENGYVKEEYIPAM
                     LEREKVMTTYMGMGVAIPHGVNEAKKEILSSGIVILQFPNGIDFDGEKAYLLIGIAGV
                     GDEHLEILSNIAVVLDDDLTERLKNSNDKQAFMEAFAN"
     gene            31835..32986
                     /locus_tag="PC471_001348"
     CDS             31835..32986
                     /locus_tag="PC471_001348"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HDF2788484.1"
                     /translation="MKKAIQFGAGNIGRGFIGGLLVKSGYHVVFADVNEEILNSINKD
                     KKYTIHIRDVECIDEVIDNISAVSSIKEEIIDEIVQAEIITTAVGPLVLTKIASTIAK
                     GIKARKEKGLTSNLNIIACENAIYASSSLKEEVLKYLNKEEVEYLEMYVGFPNCSVDR
                     IVPPGKNENPLDVTVENFYEWNVEKQGFKGEIPTIVGMNLADNLMAYIERKLFTLNTG
                     HAITAYIGYLKGYKTIEESIKDKFICDIVKSAMVESGEGLIKKYNFDSEVHYKYIDKI
                     LNRFKNPYLNDDVLRVGREPLRKLSDKDRLIKPLMTAKSYGLSVDNLILGIGAALHYN
                     NSEDTQSVELQELIKSIGVKKAVAKIANISNDEELLNNIEKSYIFMKNL"
     regulatory      33092..33193
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            33376..33948
                     /locus_tag="PC471_001349"
     CDS             33376..33948
                     /locus_tag="PC471_001349"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HDF2788485.1"
                     /translation="MKALQEKILREGSVSGNDILKVDSFLNHQIDVAFLNEIGREFKE
                     RFKGEKVDKIFTIEASGIAIASIVSQYFDNAPVVFAKKSESKNLDKDVYETNVYSFTK
                     AREYSVKVSKKYINKGENILIVDDFLANGRAALGLKDLIEQAEANLVGVGIVIEKGFQ
                     AGGALLKANDVRLESLAVVESIDNGTVKFR"
     gene            34012..34662
                     /gene="fsa"
                     /locus_tag="PC471_001350"
     CDS             34012..34662
                     /gene="fsa"
                     /locus_tag="PC471_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HDF2788486.1"
                     /translation="MRFFIDTANIEEIKEANDLGVICGVTTNPSLIAKEGRDFIEVVK
                     EISEIVDGPISAEVISLEHKGMIEEAEKLSKIHKNIVIKIPMTAEGLKAVKVLSSKGI
                     KTNVTLIFSAGQALLAARAGATYVSPFVGRLDDISQNGLDLIEEIVDIFSVNSIEAQI
                     IVASVRNPIHVLQAARMGADIATVPLKVINQMIKHPLTDKGIDSFMKDWEGASLKL"
     gene            34814..37738
                     /locus_tag="PC471_001351"
     CDS             34814..37738
                     /locus_tag="PC471_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDF2788487.1"
                     /translation="MDKILREIQREDKKNPYTDQELAEMLNVARSEVISVRKKNNILD
                     SRERRKKILIKDISKIVSENPQMSERKITEILIENGYNISRSAVSKLLKEENLNQSKI
                     GIKDKDPASSGKYVKVSKVVEEDGFEELIGIKGSLKEKVNLAKSAIMYPPNGLHTIIY
                     GETGVGKSELATCMYKYAIKNNIKEENSPFIVFNCADYAENPNLLIAQLFGVVKGAYT
                     GADANREGLVEQANNGILFLDEIHRLPAAGQEILFSLIDRGEFRRLGESSSNRKANVL
                     IISATTENPDSNLLQTFRRRIPMVINLPNMSERPKSERYEIIIKFFEREAKRVNKNFI
                     ITKEVMCALMNYKCTGNIGQLKSDIQVTCARAFSKSIFSSDKVIIDLDSLRDYIKSGY
                     YDCNCKNEKYSEFSVEDIYIDISELVSGKNRILSDNEISEIYNYAEKELKVLESKCFS
                     EEELKNAFIEKLDKKFNAIKNNKNLSDRRSRIDWGGQLKESTLEIMDKVIVFIKEQFK
                     HVNQGLYLALAIHIEHAIGRIKDGKTIINPSLDKIKASMPMEYELSRYIMGIVEDLTQ
                     VTFPEDELGYLAYYINKFCYNEESIKDKVKVVIVTHGKVGIEMSKVVNHILGIECTLG
                     IEIALTDSPSEGIEHVLEELQKIEARKGILVLIDMGSLVILGDEVEKRLGIRCKTVNR
                     VDTLLAMEAGKLATIEGKSLDGIIADLKKNKNYAMINTNKFSYRKNEYGKKNVIITLC
                     LSGVGTALNLKEHIEKQIKEYDTLIEVKPVAFLNNNLEEELNDIESEYNIIAICGTID
                     IDYKNVPFISYNEVLGKNGINKVLEHLNSKQPALNIENQLNNLIHEDLILYNFEGISK
                     EYIIDTLVSKLEEGGYVDSKYILSVYKREAMGSVVMASKVAVPHGLPENVMKPAIAIA
                     RLNKPIVWDNKFMVDLVVLLALKENNKKEIRSLFSKINDQHTLEVLLNTEDKNEIKEL
                     LS"
BASE COUNT        14975 a         4145 c         7107 g        11607 t
ORIGIN      
        1 taaaagagta ttacatagta catataaatc tatatttata aatacaataa agtaaagttg
       61 taaaggagtg ctttaaatga agttttcaga gtttaagtat gaaagaccca attataatag
      121 tatgaaaaaa gagtttttga gttgtgtaga agatataaat aattctcgta attataaaga
      181 gcaacaaaaa aatatacata agattaattt attaagaaat aaaatagaaa cgttgtcaaa
      241 tatagcttcg ataagatata gtacagacac atttaataaa ttctacaaag aagaaaaaaa
      301 ttattgggat gaatatatgc ctttgtatga agaattaaat tcatattttt ataatgccat
      361 tgtaaattca aaatttaaat atgatttgat aaaagagttt ggagagcaat ttttcacaat
      421 agtagagtat tctttaaaaa gtttttccaa agaaataata agtgaattgc aggaagaaaa
      481 taaattgtgt tcagagtata ctagactttt agcttcagct gagataatgt ttgatgggaa
      541 aataagaaat ttatctggta tggggaaatt catgtattct aagaatagaa aaacaagaga
      601 attagcaaac aaagcatatt ataatttctt tgaagaaaat gaaactaaat ttgatgatat
      661 atttgataaa ttggttaaac ttagagataa gatgtctaaa aaattaggtt ttgaagattt
      721 tgtagagctt gggtacatta gaatgatgag aagtgactac agagaatata tgataaaaaa
      781 tgttagaaaa caagtactaa aatatgttgt tccaatggca aatgagcttt atgaaaaaca
      841 agctaaaaga attggtctag aatatttaag ttacatagat gaaggcgtgg aatttttaac
      901 aggaaatgca agtttaaagg gagattcaag atacataatt aaaaatggta aaaggatgta
      961 ttctgaactg tcaaaagaaa caaatgaatt ttttgatttt atgttagaaa atgaacttat
     1021 ggatttagaa acgaagaaag gaaaaggagc tggagggtat tgtacttata taccagatta
     1081 taagtcacca ttcatttttt ctaattttaa tcaaactgct gatgatatag atgtattaac
     1141 acatgaggca ggacatgcat ttcagttgta tatgtctaga tggattgata tgccagaaat
     1201 taattttcca acgcttgata gttgtgaaat ccattcaatg agtatggaat ttataacatg
     1261 gccgtggatg gatttatttt ttaaagaaga tacagataag tacaaattca ctcacttatc
     1321 atcttcaatt aaatttatac cttatggagt aattgttgat gaatttcaac actatattta
     1381 caaaaatcct aatgtagata agtcaaaacg aaaagaaatc tggagatttt tagagaaaaa
     1441 atacttacct catagaaaat atggtgataa ttctttttta gagagaggtt gttggtggtt
     1501 taaacaagga catatattta aaaatccgtt ttattatata gattatgtat tagctcaaat
     1561 ttgtgcgtta caattttgga aaaaaatgat tcaagataga gatgcaggat ggaaagatta
     1621 tataaatatc tgtaaagttg gaggaacaaa atcattttta gatattgtga gtatgggaaa
     1681 tttgtattct ccatttgatg atggctgtat tgagtctatt ataggtgatg ttaaatcttg
     1741 gtttgatgaa ataaatgaca gcaaattata aaaaattatg agaattttat tgtataaaag
     1801 taaaaaatta aaaaatatat aaaaagtgat tatttgttgt gaaaatagtc ttgtttttaa
     1861 ctaaattgat aaaataaact aaaaaagatt tgtacttaaa aaaaaaagaa aatacaagaa
     1921 atatgcaata tcaacatttg gaaaaaaaag taaaaatgta tacaaaatac atataaaaaa
     1981 actattgcaa attttataga atgtttatat acttaagtca acagagatag aaaaaagcta
     2041 tttatagaaa acaaaggata aattgcttaa ttttaaattt tctgaaaaaa ataataatcc
     2101 ggatgaagag tatgggagac tgctttataa aaagcgaccg aagggacaag tttaatctta
     2161 cttacccaaa agattagatg aaattctcag gtaaaggtga ctgtacttgg acggggctct
     2221 gaaaatgatt ttaacaggga agacaaattg ggagatttgt ttttcctgtt ttttatttat
     2281 caagtaataa ataaaagttt gaaagagagt aaaaaaatga taataattac gaataatcca
     2341 aaagtaaaag aagaagttca aggcagagaa gttttgttta aagacacaac ttatattgga
     2401 atcttagaag caagtagaga tttgatacat gaaggttatg aacttttatc tcatccactc
     2461 tatgggagtg taaagccaaa tgaaacacct tacaggactg ttatactaaa gaaaggaaat
     2521 cgtttggata taaactcttt aaccttaatt gaagaagcaa tcatcactgc aagtaaattt
     2581 cagaataata aaaagactcc aaaatggaca gaaagtgttc aagatgattt tagagtaatt
     2641 gattatgata tattttataa tacaatacaa agaatgcagt atgaataaaa aaattgttaa
     2701 aaagttatta aaacagccta aatattaaat ctaatttaat attaaataaa atgagaaatt
     2761 taattgggga ggataagtca aaatggaaaa tgtgtatgac ttagtaataa ttggttcagg
     2821 accagcaggg cttgcagcag gtctttatgg agcaagagca aagttaaaaa ctctaatcct
     2881 agaaaaagac aagactggtg gacaaattgt aataacacat gaaattgcaa attatccagg
     2941 gtcagttcca aatgcaacag gaccaagttt aatagctaga atggttgaac agtgtaaaga
     3001 gtttggagca gaaatgctaa gagacaacat tgttgatact gagctagatg gagatataaa
     3061 agttttaaaa ggggaaaagg cagaatatag agcaaaagca gtaataatag gaacaggagc
     3121 aacaccaaga aagattggtt gtcctggaga aaaagaactt actggtaaag gggtttcata
     3181 ttgtgcaact tgtgatgcag atttctttga agattttgaa gtatttgttg taggtggagg
     3241 agacagtgca ttagaagaag caatgtattt aactaaattt gctagaaaag taacaattgt
     3301 acatagaaga caaggattta gatgtgctaa gagtgttgaa gaaaaagcaa aagctaatcc
     3361 aaagatagag tttttattag atacagtaat tgaagaaatc aaaggtgatg gaatattaga
     3421 atcagttgta tttaaaaata aagttactgg agaaactcat gaatactttg cagatgaaga
     3481 agatggaaca atgggagtat ttgtgttcgt tggattagat gctcaaactg atttattcaa
     3541 aggtaaagtt gatatggatg aaaaaggtta cataataact gatgaagata tgagaactaa
     3601 cataccaggc gtatttgcag caggagattg tagatctaag actctaagac aggttgtaac
     3661 tgcaactaat gatggagcaa ttgcatctat agtagctgag aaatacattg atgaaaaatt
     3721 tgggaactag aatctgtaaa ttaagaaata gtgtctgtaa atttaatgaa taatatttaa
     3781 aattaaaatt aatatttaaa ataaaaataa aaatttcaag ggggaataag aaaatgttag
     3841 atttagataa ggctacattt gaagaagaag ttttaaatgc agaaggtttt gtatttgttg
     3901 atttttggag tgaaggttgt gagccttgta aagctttaat gccagatgtt cataaattag
     3961 ctgagactta tggggataaa ataaaattct gtaagatgga tacaacaaaa gctagaagat
     4021 tagctataaa acaaaaagta ttaggtcttc caactatggc tatatacaaa gatggagaaa
     4081 aagtagacga agttactaag gatgatgcta cagttcctaa tatagaaaac atgattaaaa
     4141 aatatcttta atatgttgat aaattattaa cattaaaaga aaaaatctat ttttatctga
     4201 agataagaag aaaaaggttt ttatgtccaa ctttgttaaa aaaataattt taaataaaat
     4261 aattttttta caataaatat tttttagtaa taaaataaag atttaaggca taaaaacaca
     4321 ataatatatc tttaaaaaaa taagataaat tgtaaaatga tataattttg agaaaaactc
     4381 aaatgcatat acacaaatta aataacagga ggtgcaagta tgcgtcttga attagggaaa
     4441 atctttataa aagatattca atttggtgat gtaacagaag taaaagatgg agtattattc
     4501 atcaataagc aagagatgct acaagaaatt ggtggagatg agcatattaa atctatagat
     4561 atagaattag ctcgtccagg tgaaagcgta agaataacac ctgtaaaaga tgttattgag
     4621 ccaagagtaa aagttgaagg taatggggga atcttccctg gtatcatgtc taaagttgac
     4681 actgtaggag aaggaaaaac tcatgcttta aaaggtgtag ctgttgtaac tactggaaaa
     4741 atagtaggtt tccaagaagg tataatagac atgactggag aaggagctaa gtacactcca
     4801 ttttctaaat taaataatgt agtagtaata gctgaaccta ttgatggttt aaaacaatac
     4861 gctcatgaaa aagctgttag aatgatagga ttcaaagcag caatgtactt aggagaagta
     4921 gcaagaaatc ttactccaga tgaagtttca gtatatgaaa ctaaacctct tcttgaatca
     4981 ataaaagaat atccagagct tccaaaagta ggatatgttt acatgcttca gactcaaggt
     5041 ttattacatg atacttatgt atatggagta gatgcaaaac aaatagtacc aactttatta
     5101 tatccaacag aattaatgga cggagcaatt gtaagtggta actgtgtttc tgcatgtgac
     5161 aaaaacccaa gttatgttca tataaacaat ggggttgttg aagacttata tgcaagacat
     5221 ggtaaagata taaactttgt aggtgtaatt ataactaatg aaaatgttta tctagcagat
     5281 aaagaaagat cttcaaactg gacttctaaa ttatgtaaat atttaggatt agatgcagtt
     5341 atagtttcac aagaaggttt tggaaatcct gatactgacc ttataatgaa ctgtaagaag
     5401 atagaaatgc aaggtgttaa aacagttata gttacagatg aatatgctgg acgtgatgga
     5461 ggttctcaat cacttgcaga tgcagatgta agagctgatg ctgttgtaac aggtggaaat
     5521 gccaaccaag ttgtagttct tcctaagtta gataaagtta ttggacattt agaagtagta
     5581 gatgtaatag ctggtggttc tgatggaagt ttaagagctg atggaactat tgaagttgaa
     5641 atccaagcta taacaggtgc aactaatgaa acaggatttg gtcatttaac tgctaaagga
     5701 tattaagaag tattttatta aacgattgag taatgttaat aaaacatatt atagataatt
     5761 ataataagtg ttatagatat ataaaaatat attagaaata taataaggag gattattatg
     5821 agtttactta gtaataaaaa ggttcttata ataggtgacc gtgatggtat accaggacct
     5881 gcgatagaag aatgtgtaaa aacagtagaa ggagcagagg ttgttttctc atctacagaa
     5941 tgctttgtct gaacagctgc tggggctatg gacttagaaa atcaaaacag agttaaagat
     6001 gctgctgata aattcggagc tgaaaatgtt gtgattttac taggtgctgc tgaagccgaa
     6061 gctgcaggtc ttgcagccga aacagtaact gctggagatc caactttcgc tggaccactt
     6121 gctggagttg ccttaggatt aagtgtttac cacgttgttg aggaaccaat aaaatcatta
     6181 tttgatgaaa gtgtatatga ggaccaaata agtatgatgg aaatggtttt agaagttgaa
     6241 gaaatagaag aagaaatgtc tggtataaga gaagaatttt gtaaatttta atgcctaaaa
     6301 gcaaaagggg tgaaactaag agatgggaaa actaaaagca gtacattata ttaatcagtt
     6361 ctttgctggt ataggtggag aagaaaaagc tgatactaag ccacatgtgg cagaaacttt
     6421 acctccaata agtttacaac ttgataaatt attaggtgaa gatattgaaa tagttggaac
     6481 agtagtttgt ggagatagct atttcaatga aaatatagat agtgcaagtg aagaagtttt
     6541 atcaatggta aaaggttttg aaccacaact tttcatagct ggtcctgcat ttaatgctgg
     6601 tagatatggg gttgctgctg gaacaataac taaagttgta aaagatgctt tgaatatacc
     6661 agctttaaca ggtatgtatg ttgaaaatcc aggagcagat atgttcaaaa aggatgttta
     6721 tgtagtagaa acttctgatt ctgcagcagg tatgagaaag gcacttccta agatagctaa
     6781 acttgcagta aaacttgcta atggagaaga aattggaact cctaaagatg aaggatacat
     6841 agctagagga ataagagtta actacttcca tgaagacaga ggttctaaga gagctgttga
     6901 catgttagtt aagaaaataa aaggtgaacc atttgaaact gaatacccaa tgccaaactt
     6961 tgatagagtt gacccaagta aagctgtaaa agatttatca aaatgtaaaa tagctttagt
     7021 tacttctggt ggtatagttc caaaaggaaa tccagataga atagagtctt cttcagcttc
     7081 taaatatgga acttattcta tagcaggagt tatggattta acagaagaga cttatgaaac
     7141 tgcacatggt ggatatgacc cagtgtatgc aaatttagat gcagatagag tattacctgt
     7201 tgatgttctt agagatttag aaaaagaagg tgtaattgga aaattacatg agactttcta
     7261 tacaactgta ggtaatggta catctgttgc aaactctaaa aaatatgctt ctgaaattgg
     7321 agcagcatta gtggctgatg gtgtagatgc agtaatatta acttctacat gaggtacttg
     7381 tactcgttgc ggtgcaacga tggtaaaaga aatagaaaaa actggacttc cagtagttca
     7441 tatgtgtaca gtagtaccta tttcattaac agtaggggct aatagaatag ttccaactat
     7501 agcaatacct catccacttg gaaatccagc gcttgaccct acagaagaaa aagccctaag
     7561 aagaggcctt gtagaaaaag cattaaatgc tttaactaca gaagtagatg gtcaaacagt
     7621 atttgaaaaa taaaaataac acacacatat attatataga taaaaatata aacacacact
     7681 aaaaataaaa aataactata cacaaaaaaa taatatagat ttcatgaatg gataaattgg
     7741 agttattcat tcatgaaatc ttaaaaaatg ccaaaataca taaacaataa tagaatcaaa
     7801 tatacaataa aatacaatga ttagagcttt taataagttt aataagatta aaaaataagt
     7861 gttcataaaa tttatggagg tgcacaaaat gacttatcca gttttaaaag gggcaggcta
     7921 tgtacttatt catactccag atatgatagt gcaaaatgga agtacttgta cagttgaaag
     7981 agcaacaaat ccagattcag aatttttaaa agaagtaagt aatcatataa gaagttatga
     8041 agatgtagtt aactacatgc caaatcaagt ttatatagga aatagaagac cagaagaatt
     8101 aagagattta ccaatgccat ggtgtgaaca aaagattgag ggaactagaa atggtaagtt
     8161 tggagaaata atgcctcaag atgagttcat agctttaatg caaataagtg atgcatttga
     8221 tttagtaaaa ttatcacaag aatttattga tgaagtaaag ccaaaaatcg aaaataacta
     8281 ccctgaaatt gcaccatttg taggtaaatt aaagggtgat gatatagaag aaggtaaaga
     8341 attagtagca acacacatag ctgaaggatt ataccatgat ggaaaatttg ttggttatgt
     8401 aaaaagagca catgatgtag atgttaactt aaatgcccat acaatgtttg aaaatctagt
     8461 agtaaaagca tctggagttt tatctgctat tcaaatgcta agacatagca aaatagaccc
     8521 tgctgaaata gattatgtta ttgagtgttc agaagaagct tgtggagata taaaccaaag
     8581 aggtggagga aactttgcta aatcaatagc tgagatagca ggacttcaaa atgcaactgg
     8641 ttctgacaca agaggatttt gtgcagcacc aactcatgca ttaattcaag cagcagcttt
     8701 agtaaaagct ggtatacata aaaatgtaat ggtagtagca ggtggagcaa gtgctaaact
     8761 tggaatgaat gctaaagacc atgttaaaaa aggtcttcct gtacttgaag atgttgttgg
     8821 aggatttgca gtacttgtat ctgaaaatga tggtgtaaat ccagttataa gaactgattt
     8881 aacaggtaag catactgttg gaactggttc ttcaccacaa gcagttatga cagcacttat
     8941 aacttcaggt cttgatagag ctaatttaaa aataacagat gttgatgtgt attcagttga
     9001 aatgcaaaac ccagatataa ctaagccagc aggagcagga gatgttccag aagcaaacta
     9061 caaaatgata ggggcacttg ctgttaaacg tggagactta gaaaagaaag aattgaaaga
     9121 ttttgtttct aacaaaggat taccaggatg ggcaccaact caaggacata taccatcagg
     9181 agctccttat ataggatttt taatagatga tttaactaca ggaaatagaa atagagctat
     9241 gatagttgga aaaggtagtt tattcttagg aagaatgact aacttatttg atggagtatc
     9301 ttttatagct gaaagaaata caggtgttac tgaagagact tcaggaatat ctaaggatga
     9361 aattaagaag ataatagctg aatctatgaa aaaactagca ttagatatgt tagaagaata
     9421 ggggtgagga tatgtcaaaa aaagtaatcg cagatgtatt cttagaagta gctaatgcaa
     9481 tagaaagcgg cgaatttggc aaaaaagtaa aaattggtgt tactacactt ggaagtgaac
     9541 atggtgtaga aaacatggtt aatggtgcac aattagcaaa atctaattta tttgatattg
     9601 tgcttattgg accaaaagtg gaaactgacc ttgaagtagt tgaagttaat gatgaaaaag
     9661 aaatgcatgc aaaaatggaa gaattattag attcaggata tattgatgct tgtgttacta
     9721 tgcattataa ttttccaata ggtgtatcta cagtaggtag agtaataact ccagcaaaag
     9781 gaaaagaaat gatacttgca acaacaacag gaacaagtgc tactaataga atagaggcaa
     9841 tggtaagaaa tgccatttat ggtattgcta cagctaaatc aatgggtaac aaatgcccaa
     9901 aagttggaat acttaatgtt gatggagcaa gacaagttga gaagtgctta aaagaattaa
     9961 aagataatgg atatgacatg gagtttgcgg attcaattag agcagatggt ggatgtgtaa
    10021 tgagaggtaa tgatttactt gtaggtgcac ctgatgttat ggttactgat acattatctg
    10081 gaaatatatt tatgaaagta ttttcttcat atactacagg tggagattat gaagctcaag
    10141 gctttggata cggacctgga gttggagagg actatgatag aaaggtactt atagtatcaa
    10201 gagcatcagg ttctccggta gtagcaaatg ctttaaaata cgcttatgat gttgtaaagg
    10261 gagatataag taatgttgca agaaatgaat ttgcaaaggt taaaaaagca aaatttgatg
    10321 acattatatc ctctcttact aaaaaagaag ttaaagctga aaaagtagaa gttaaaatgc
    10381 cagataaaga aattgttact cgtcaaatag ctggtgttga tataatggat ttagaagatg
    10441 cagtatctga gttatggaaa aacggaatct atgctgaaag tggtatgggt tgtacaggtc
    10501 caatagtttt agtaaatgaa gctaaaggag acttagcagt tgaaacttta gttaaagctg
    10561 gatatactgc aaaataagta cattttttat atgtaaataa gaacatatag ttataatttt
    10621 attgattata attatatgtt ctttttattt tatatcttgt caatatttat atattaacac
    10681 aatccattta tttgattctc tagctaattt attatattta ttcagttaaa attaagcttt
    10741 ctaactaaat aaaaactaat gaagtattaa aatgaaattg ataaaatatg aaaaaatttt
    10801 aggaataaag tatctgttag ataaaaataa gattaattag tgatataata taattaagat
    10861 aaaatttatt atttggaggc aaaatcatga gcagagtaaa aaatgtagta gaattgttgg
    10921 aaactaaagg tgttgatgca ctatatctaa caaagaaaac taatgtaaac tatataagtg
    10981 gttttcctga tgaagaagca tatgcagtga tttgcaaaga tggaaacttt ttagttactg
    11041 atagtagata tatggagctt gcagaaaagg tttgcaaaga ttttgaaatt ataaattggc
    11101 ataattttga tagaagtgtg gcaaaggcag ttaaaagtgt atgtgataaa gtgggtataa
    11161 agaaacttgg gtttgaaaga actaatatag tgtttgataa atatgaagaa cttaaaaatc
    11221 tgattgagaa ggataatgga gaattaatac ctacagaaaa tattgtagag actttgagat
    11281 atgtaaaaga caaagatgaa atcaaaaata caagaaaagc ttgtgaaata gcggataagg
    11341 cattagaaga attgattcca catataaaag ctggagttag cgaaattgaa ctggctacaa
    11401 agttagagta ctttatgaaa atgaacggag cacaaaatat agggtttgaa actatactga
    11461 tatcaggagc aaagacttca ttgcttcatg gtaagcctag tgataagatt atagaaaaag
    11521 gtgattttgt acttatagac tatggcgcta tgtataatgg ctatatatct gatacaacta
    11581 gaacttttat agttggagga gcttctgaaa agcaattaga aatatataat ttagttaagg
    11641 aagctcaaaa tgtaggggtt gagaacatga aagctggtgt acatgcgaca attccagatg
    11701 ctgagataag aaaagttgtc aaaaaatatg aagactatta ctatcaaggt ataggacatg
    11761 gagtcggtag agatgttcat gaagaacctt tcattggtaa ttatggtgat aaaatcatag
    11821 aagagggatg tataataaca atggaaccag gtatttattt ccctggatgg ggtggagtta
    11881 gaatagaaga tactgttttg attactaaaa atggtccaga aagacttact aaattcccta
    11941 aagatttgat gattttagac aaataattta atttatatat atttttttac ataaaatata
    12001 acataatctc actattaagc tctatttaca tattatagta aagagggctt aattttattg
    12061 tgagaggtga tagtatatat gtataaaaaa cataaattta taaaaaatat aaaaaaagcc
    12121 tggtataaaa tcaaattaat tgatagatgc cttataattg taatggcaat attaatgttt
    12181 caatctatat ataatttatt tgttaatgaa gtaaattcac aagatactac aactattgat
    12241 gtagtagtaa gaaccacttc agcagctata tttgggtatt ttttaagtgc taattttata
    12301 aagagacctt taaggaagta tacaaatact gatgtatctt caaatctttt tatagataat
    12361 aatttatccc caaaaggtca agagtctaat caaaataata taatgaatgt caaaaatacc
    12421 attggattta cttcagaagc tgataattat gaaaagaaaa tacctattaa taataatgaa
    12481 ggttttgaag aaggagaaac tagtgaattg caaattatta tagctactgt aatttgtata
    12541 gtagcattaa cagtactttt tgttgtaaga aattttacaa ctacaacatc agcttcttta
    12601 ggtactattt cacagatgag agattttgtc tcaggatgcg tgggtttttt gctaggttgt
    12661 ccaagtaaac ctgattaaaa aagtattatc aataaaaggt attttaaaaa gtatcagtat
    12721 aataatattt agatttaaat gtatacaatt ataattctaa acattctata ttgtattttt
    12781 atactgatac tctaaactat actatttatt aaaatatttt aacattgtat ttaaacaaaa
    12841 tattatatct gtataatata aatttagttt atatgaatag atgccatata ctcaataaaa
    12901 agtttcatac tatgagatat gtattgattt cttctccaaa gaaccccaat attcgcagta
    12961 attgcacgtt ctaatggtat tgaacagaga ttttctgtat tttgaaatat ttctttatac
    13021 aatattgttg aagccaagtc attttctagt aaataacgta tggtagaaag ttgatttgag
    13081 tgtaaaatta tatttggctt agctttttca agtctttttg taataatctt atagtgataa
    13141 gaaccttcag ataagattac aaaaggtaca ttttttaaca tataattcga tataatggga
    13201 tgttttgcca attcattttt gggatttgta actaagtata attctgtttt atagattgtc
    13261 tttgaatcta tatcatcata aatatcactg tcaattatac caatacaaaa atcaagtgta
    13321 gattcttcaa tcattttagc tccaacaaat gttggaactt caaaaatttt aagctctata
    13381 tctggataaa ttacattaaa tctaggaagt atttttgaaa gcaaaaacgt acctaaaact
    13441 gtaggaatac ctattttgag agaagctttt ctcttttttc ctaaatcaag tgcgtcacat
    13501 tgaaaatcct caaattgttt tataaaatgt tttgacttgt ctaaaaaaat ttttccttca
    13561 ggcgttaact ctattttatt acctattctg taaaaaagtt caaagccaag ttctttttct
    13621 aattctttaa ttgctaatgt tatggatggt tgagaaatat gaatactttc cgcagcctta
    13681 gtcatgtttt tatatttaca gacagtttga aaatagtata aatagtttat attcatttgg
    13741 tattagttcc tttcatttac atattagtga caagtatata attaacaata gcataagttt
    13801 tgtatattgt tgtaaaaaaa tagaatagtt ttaaactatc atagcataag tttttgatat
    13861 tttcaatcta ttaagaatag tgctattatt aaattaacaa aagagaatag ctggtatggt
    13921 ttagagtaaa tcaatctata ttacagttat atcaacgaat atcaaaaaat tatctattag
    13981 aaaataaaaa ttatttttac aattaaattg ttaattaaat cataaagaaa aaagtgatat
    14041 taacaagata aaattttttt aatcagaaat gttaatttaa taacaaaata gagtattggg
    14101 gagaaaggag aaatatattt tttaataata tttgttaatt taataacgaa aaatgaaagg
    14161 gagtgattta gtgaagtttg aattgatgga ttttttaatg aatccttttg tccttatgtt
    14221 tgctgcagta attacaggaa ttttatttgg aaaaattaaa tttgggaagt ttaattttgg
    14281 ggtatcagga gcattattta caggattatt tattggatgg cttgcatata gcttggggaa
    14341 tctaataata gcaaaagggg aaacagcagc aggatataag gcagcaactg taatgatggg
    14401 taatggaatt atttcttctg acttctttga tttcttttta ataatatttg ttgcagcagt
    14461 tggtctattg gcagcaaaag atatgaaagc tgtattaaag aagtatggag caagatttgt
    14521 aattttagga gttcttataa cttttatagg tggctctatg acttatgcaa tgactttact
    14581 atcaagtgat aaaggaagta gtgcttatga agtttctggc gtttacacag gagcattaac
    14641 aagttcgcct ggattagctg ctgctttaga aacagctggt aaacatgcag aagatgtatc
    14701 aaaagaattt gaaaaagctt caataaaaga taaaaaagaa attttaaaag tagtcgaccc
    14761 agaaggcaaa ttggatgtga atacaactac atcactgact caagagcaaa tagataagta
    14821 tattgcatat gctgaagctg gtgttggtat aggtcatgca gtcgcatatc cttttggagt
    14881 tttaatagta atcttaggtg taaatttcct tccaaaatta tttaggatgg acctaaaaga
    14941 agaaagaaga aaatatgaga aggaaatgaa ggaagcaaga gatagtgttt ctggtaaaac
    15001 gataccagaa gttccattta atataataac atttttcttg acatgtttag ctggatactt
    15061 agttggagga atacatgtat ttatgggacc actagggtac ttcacactag gcgcaacagg
    15121 aggttcacta attgtatctc tagttcttgg atatataggt aagatagggg tagtaaattt
    15181 cagaatggaa gaaaaggttt taaatatttt aaaacaaata gggctagtat ttttcttagc
    15241 aatagttgga ttaagatatg gtggaaaagt agttgattca attatgacat ctggtatgca
    15301 cctagcctta gtagcaatag cagtaggtgt tacagctatg atgataggat tcttagttgg
    15361 caaatacgta tttaaattaa attggatttt attatctgga gctgtctgtg gtggtatgac
    15421 atcaacacca ggtcttggag ctgctgttga tgctcttgat agtgatgacc cagcagcagg
    15481 ttatggtgct acgtatccat ttgcattatt aactaaagtt atattggtta tagttttaca
    15541 caaacttcca atgtaaataa gattttatat ggggggatat ttatgaataa tgaaggagca
    15601 tttgaagaat taagtggaag attaagaaat aaagaattgt gtgatttaat aatgaatgca
    15661 gatgaagctg ctaaaattat agaggatggc atggttattg gagttagtgg atttacacct
    15721 tcaggctatc caaaagcagt accattagca gtttcagaaa gagctaaaag tggagaaaag
    15781 ataaaattaa ccgtatattc aggagcatct ttaggtccag aagttgatgg agcatggtca
    15841 gaagcaggta taattgaaag aagacttcct tatcaaacta attcaatact tagaaataat
    15901 attaataaag gtgttgttga ttatatagat atgcacctaa gtcattctac tcaattttta
    15961 aattatggaa ctatacctaa agtagatgta gctatagtag aagcacttgc aataactgaa
    16021 gaaggaaata ttataccaac tagtgggata ggtaattctc catcttttat aaaaagtgca
    16081 gataaagtaa tagtagagat aaatcttgca aaaccaatgg aaatggaagg tatggcagat
    16141 atatatatta cggaaaatcc accaaataga aaaccaatag aaataaatca tcctaaggat
    16201 agaattggaa caacatatat accttgtggt ttggataaaa ttgcaggagt agtaattacc
    16261 aatatgcaag ataagacccg tccattggga gttgtagatg aagcttcgaa aaaaatatca
    16321 aataatatta ttgctttttt aagagaagaa gtaaaggcag gcagattatc aaagaattta
    16381 cttcctcttc aatctggtgt aggaagtgta gcaaatgcag tattatatgg attatgtgaa
    16441 tctgaatttg aaaatttaac ttgttataca gaggttgtac aggactctat gttggattta
    16501 ataagaatgg gtaaagttac aatggcatct actacatctg taagtccatc tccagaagga
    16561 ttaataaaat ttgaaaaaga tattgatttt tttaaggata agattatatt aagacctcaa
    16621 gaaattagta acaatccaga aatagccaga agaattggtg taattgcaat gaacactgca
    16681 attgaagttg atatttatgg aaatgtaaat tctactcaca taatggggtc aaagatgatg
    16741 aatggaattg gtggttctgg tgattttgct agaaatggag caataactat atttagtact
    16801 gagtcaattg caaaaaatgg tgatatatca tcaatagtac ctatggtatc tcacgtagac
    16861 cacactgaac atgatgtaat ggtaattgta acagaacaag gatatgctga tttaagaggt
    16921 cttgcaccta gagaaagagc tataaaaata atagaaaatt gtgcccatcc agattataaa
    16981 gaacaactta gggattattt aaatagagct tgtaaaagtg gagcaaaaca aacacctcac
    17041 atattggacg aagctttatc atggcatagt aaatttatga gtacaggtac aatgaaaaag
    17101 gctgaaactt ttaaaagtgc attataaaat atttaataaa ttggaggtag attttaaatg
    17161 gaaaaagcag tggaaaactt tgaagattta agtaaggaat acattaatgg ttatattgag
    17221 agagcaagaa aagctcaaag agagtttgaa tgttatactc aagaacaagt agataaaata
    17281 gtaaaaatag ttggaaaagt agtgtattat aatgctgaat atttagctaa acttgctgta
    17341 gaagagactg gaatgggagt atatgaagat aaggtagcta aaaataaaag caaagcaaaa
    17401 gttatatata acaatttaaa agataaaaaa tcagttggta taattgatat agatagagaa
    17461 actggaataa caaaggtagc taaaccagtt ggagtagtag cggcaataac tccttgtaca
    17521 aatccaatcg ttacacctat gagcaatgca atgtttgcac ttaagggaag aaatgcaatt
    17581 attatcacac ctcatcataa agcaattgga tgtagtacaa aaactgtaga gatgattaat
    17641 gaagaattag aaaaaatagg agctccagaa aatttaatac aaatacttga ccaacaatca
    17701 agagaaaata caagaaattt aatttcatca gcagatgtag taattgccac tggaggaatg
    17761 ggaatggtaa aagctgcgta ctcaagtgga aaaccagcac ttggtgtagg ggcaggaaat
    17821 gttcaatgta tcatagatag agatgtagac attaaagaag cagtacctaa aatcatagca
    17881 ggaagaattt ttgataatgg tatcatttgt tcaggtgaac agtcagtaat agtagcagaa
    17941 gaaatgtttg ataaaataat ggatgaattt aaaaataata aaggttttat agttagagat
    18001 aaagtacaaa aagaagcttt cagaaatgct atgtttgtaa ataaatctat gaataaagat
    18061 gcagtaggtc aatcagttca tacaattgca aaaattgcag gtgttgagat accagaagat
    18121 acaaaaataa tagtaataga agctgatgga ccaggagaag aggatattat tgcaaaagaa
    18181 aaaatgtgtc cagttatatc tgcatacaag tataaaagtt ttgaagaagg tgtagctatt
    18241 gcaaaagcaa atttaaatgt agaaggaaaa ggacatagtg tatctataca ttcaaataca
    18301 gttaaaaata tagaatatgc aggagaaaat atagaagtat caagatttgt tattaatcaa
    18361 tgttgtgcta ctagtgctgg aggaagtttc tttaatggtc ttgcacctac taatactcta
    18421 ggttgtggtt cttggggaaa taacagcatc tctgaaaatc ttgattataa gcatctaatt
    18481 aatatttcta gaattgctta ttatatgcca gaaaatgaag tccctacaga tgaagaactt
    18541 tggggataaa atttagacaa aaaatattca ttctaaatta aagtaggaat atattttaat
    18601 ttagaatgaa tttaagacat aaattaaatt taaattagtt tattggacta aatttaaaat
    18661 attaagagac atatagaaga aggaagtatt ataatttaaa ataaataaaa aaatctataa
    18721 aagagaagga gtggttttat tatggcatta atgacaggag ctcaatatat tgaaagtttg
    18781 agaaaattaa atactaaggt ttatatgttt ggtgaagagg ttaagaattg ggttgaccat
    18841 cctatgatta gaccatctat aaattgtgta gcagcaactt atgacttagc tcatgaccca
    18901 gaatatgcag atttaatgac agttacttca aatatcacag gtgaaaaaat aaatagattt
    18961 ggtcatcttc atcaaagtgt tgatgatttg ataaaaaaag taaaaatgca aagactatgt
    19021 ggtcaaaaaa cagcttcttg tttccaaaga tgtgttggta tggatgcgtt taatgcagtg
    19081 tattcaacaa catttgagtg tgataaagcc catggaacta attatcatga taattttgta
    19141 aaatacttaa cttatataca agaaaacgat ttagtagttg atggagctat gactgaccct
    19201 aagggagata gaagtctatc acctagtgct caacctgacc cagatatgtt ccttcatata
    19261 gtagaaagaa gagaagatgg gataattgta agaggagcaa aggcacatca aactggttca
    19321 ataaattcac atgagcacct tataatgcct actataagta tgactgaagc agataaagac
    19381 tacgctgtat catttgcagt tccttcagat gctgaaggag tgtttatgat ttatggaaga
    19441 caatcttgtg acactagaaa attagaagaa ggtgcagatg ttgatttagg aaataaggag
    19501 tttggaggtc aagaagcttt agtagtattt gataatgtat ttgttccaaa tgatagaatt
    19561 ttcttatgtg gagaatggga tttctctggt atgttagtag agagatttgc aggataccat
    19621 agacaaagtt atggtggatg taaagtagga gtaggtgatg taataatagg agcagcagct
    19681 ttagcagctg attataatgg agcaaacaaa gcttcacata taaaagataa actaatagaa
    19741 atgactcacc ttaatgaaag cttatattgc tgtggtatag catgttcatc agaaggtcac
    19801 aaaacagaag ctggaaacta tcaaatagat ttacttcttg caaatgtgtg taaacaaaat
    19861 gtaactagat ttccttatga aatagtaaga cttgcagaag atatagctgg agggcttatg
    19921 gttactatgc cttcagaaaa agactttaaa tcagacctta aagttggaac aagtggaatg
    19981 actattggcg aagtatgtaa caagtatttt aaagcttctt cagtggcttc aacagaagaa
    20041 agaatgagaa tattaagatt cttagaaaat atatgccttg gttcatctgc tgttggttat
    20101 agaactgagt caatgcatgg ggcaggttca ccacaagctc aaagaataat gatttcaaga
    20161 caaggaaaca taaatcaaaa gaaagaatta gctaagaaaa ttgcgggaat aaaaaaagag
    20221 gaagctttaa atttataaaa agttgatata taaagggaaa ttatatatta atgtgtgtta
    20281 attaatgaaa aaatatgtag tataagagat agttataaaa ttatggtggt ttttagctaa
    20341 gtaaagaaat ttttaataag gagaatttac acatgacctg tggagttaga ttttgtgggg
    20401 gctgtaaccc tagatacgaa agagggaagg ctcttacaat aatcaaagag cacttcaatg
    20461 gtagggtaga ttttagtata gcacaagaga acgtcgagta tgatttgtta cttataattg
    20521 gaggatgcgc aactctatgc gcatcctatc aacaatataa agtgaaaaat gaatgtgtta
    20581 agatgtggga ttttagccat atagaaagag ttataaatgt gattgaatca gcaatttgtg
    20641 aaacttaaaa aagctaaatg gggggatttc attgagttgg caagaattgt atcaaagtaa
    20701 attatgtagt gctacagaag ctgtaaaaca gattaaaaac ggtgatactg tagtatttgc
    20761 acattgtgta ggtgaacctc ctgcactagt agaagctatg attgaaaatg ctgaacaata
    20821 taaagatgtt gagattaaac atatggtaag cttgggtagt ggtggatata ctgctaaggg
    20881 aatggaagct cattttagag taaatccaat gtttgtaagt ggaaatgtaa gaaaggcaat
    20941 tgaaaatgga gatggtgatt ttacacctgc attcttccat gaagtaccaa agttgcttag
    21001 agaaaaaaga ttaaaatgtg atgttgtttt agcacaggta actccaccag atgaacatgg
    21061 gtattgtagc ttaggaacat cagttgatta tacttatgaa gccattaaaa ctgctagaac
    21121 agtaattgtt caagtgaatg accagtttcc tagaacctat ggtgaggtag tccatgtatc
    21181 agagtttgac tatatagtgg aaaaatcaca acctttattt gaactacaac ctgcaaagat
    21241 tggagaagta gaagaagcaa ttggtaaaaa ttgtgcttca ttaattgaag atggctctac
    21301 tttacagcta gggattggtg ggataccaga tgcagttatg ttatttttaa ctgataaaaa
    21361 ggatttaggg attcattcag aaatgatttc agatgggact ttggcccttt atgaaaaagg
    21421 tgttataaat ggtaaatata aaaattttga taaagaaaaa atgacagtta ccttcttaat
    21481 gggtactaaa aaattatatg actttgcaaa taataaccct gcagtagagg taaaaccagt
    21541 agactatgtg aatcatccag caattataat gaaacaacat aagatggttt ctataaattc
    21601 tgctatacaa gttgatttaa tgggacaggt agttgcagag gctatgggac ttagacaatt
    21661 tagtggtgtt ggaggtcaag ttgactttat acgtggagtg tctatgggtg aagatggaaa
    21721 ggcaattata gctatgcctt caataactac aaaaaaagat ggtacagtaa tttcaaaaat
    21781 cgtatctatt gtggatgaag gtgcacctat aacgacatca agaaatgatg ttgactatat
    21841 tgtaacagaa tacggtatag cagaattaaa aggaaaatct cttagagaaa gagcaagaaa
    21901 tttaataaat atagctcatc caagtgtaag agaacatcta gcagtagaat ttgaaaagag
    21961 atttaaggag aaatattaat aatagagggg tgttaataat ggaagcactt agagtagttc
    22021 caaagatatt ttactttgat acatttaaag aatttaatga agaatttaaa ataggaaaaa
    22081 atgatttagt aattactaat gagtttatat atgagccata tatgaaacct cttggaatag
    22141 atacaaattt aatttttcaa gaaaagtttg gaacaggtga accatctgat gaaatgatag
    22201 actccatgac aaaggaaatg aagaaataca attttgatag aattattgca tttggaggag
    22261 gaacaatagt tgatatatgt aagatattag cacttgatgt tccagaaaag tctattgatt
    22321 tatttgaagg agaagtatca cctaaaaaag taaaagagtt agtagtagtt cctacaacat
    22381 gtggtacagg tagtgaagta acaaatgtag ctattgcaga gttaaaatct aaacatacta
    22441 aaaaggggct tgcagtagaa gaaacatatg cagactatgc agtgttaatt ccagagacag
    22501 taaaagggtt accttacaaa ttctttgtta caagttcggt agatgctctt atacatgcta
    22561 ttgagtctta tctttctcca aaagcaagtc catttacaga gatgtattct ctacaagcaa
    22621 taaaaatgat tatggatggg tataagaaga tagttgataa aggagaagaa gagagattta
    22681 atcatcttag agactttgta cttgcatcaa attatgcagg aattgctttt ggaaatgctg
    22741 gttgtgcagc agttcatgca ctatcttatt caataggtgg agctttccat gttgcacatg
    22801 gtgaagcaaa ttatcagttc tttactgaag tatttaagat gtattctaga aaaaaaccta
    22861 atggaaaaat tataaaatgt acaaaaattt tagcagatgc ccttgaatgt gaccctaact
    22921 gtgatgttta tggagaatta gaaaagtttt taaataaatt aatagctaaa aaagctttga
    22981 gagaatatgg aatggtggaa agtcagatag atgaatttac agatagtaca atagcaaatc
    23041 aacaacgact acttgctaat aattatgtag agcttagtag agaggaaata agagagatat
    23101 ttgcaaatct ttattaatac aattttttat ttatatgtat agtcaacata aacaatgcta
    23161 attatattta tgtacaaata aaaataaggg aacttaaatt ttaattaaag ttaagttctc
    23221 ttatttttat atggagttaa atataagtat atttttaatt taaaatatgc ttatatttaa
    23281 gtctattact ttatttgaaa caatttgtag aatttcaaat tccttgacag taataagtaa
    23341 gtaattgtat aatcaaactg caatatatta ataatattag tgaaaatgaa aggattataa
    23401 aatatgtata aatcagaaaa tttttcaaaa aaatctgtat taataaattc atttttaagt
    23461 aaatttttaa aaataattgg atggggtttg gtaagcttat ttacattatc agccttagta
    23521 ctttcaacta tttccattat aattggatgg gctgtaatac ttcctattca agtatgtgca
    23581 gtatttttac ttataggagg agtttgtatt tttaaaggta agagattagg agaccaaatt
    23641 tctcgttata aaaaatattg tacaattata aacaatagaa atattattcc agtagaactt
    23701 attgcagaaa acacatctaa atcattaaat tttataataa aagatgtaca aaagatgata
    23761 gacaaaaact actttataaa tacttatatt gataaaagaa ataatcagat tgtgttaaca
    23821 aatgaagatt ttgttccacc aagatatgaa gatgtagcat atgaagtcaa agaagaagaa
    23881 aagactgatg aatccaatga agttgataca attataaata aaggaatgaa ttatctaaca
    23941 caaataaaag aagctaataa aaatattaaa tcagaatcaa tgggcgataa aattgtacaa
    24001 gtagaagatg ttacaagtaa aatatttgat gttgtaaaac atgacccatc aaaacttact
    24061 cagatacaaa aatttatgga ttattatctt ccaaccactt taaaactatt aaattcatat
    24121 catacattgg aagaacaagg aattgataga gaaaatataa caacaactat ggaaagtatt
    24181 gaaaatacaa tgaatacgat tgtagtagct tttgaaaatc aattggacta tctgtttgaa
    24241 gatgaagcca ttgatatttc tacagatatt actgttttag agaatatgtt agtccaagaa
    24301 ggattaacga gtggtaaatt ttaacttcaa tagaaaaaat attctgttat ttttaaacaa
    24361 ttaattagtc ataaagtata aaatgtgtat tatataagtt aatcagtact ttatgaagga
    24421 agaggagaga aagatacaat gaataattta gatgatatac cagttatgcc aactttgaca
    24481 cttgacccat ttggagaaga atcaaacact aatgatatag ataattctga cttattaatg
    24541 aaaaaggatg aaaaagaccc agaagaagaa aaactgtctg aatctgaacg caagatggta
    24601 aaggaatttg cggaaaaaat agatattaca aatacaaata tgattttgca atatggagct
    24661 ggagctcaaa aaaaggtagc aagtttttct gaaacagctt taaagagtgt aaaaacaaga
    24721 gatttaggag aagttggaga tatgcttaca aatctagtta ctgatttaaa agcattttca
    24781 gcagatgaaa aagaacaaag tggtttttta ggaattttta aaaaggcaaa taataaaatt
    24841 tcaaatttaa aaactaagta tgatagtgca gaggtaaatg tggataaagt aagcaaagag
    24901 cttcaaaagc atcaggttaa aatattgaaa gatatagcaa tgcttgataa gatgtatgaa
    24961 ttgaacttag cttactcaaa agagcttact atgtatatta tagcaggaaa acaaaaatta
    25021 aaggatatga aagaatatga aatgccaaag ttgagagaaa aagctcgttt atctggttca
    25081 acagaagatg ctcaatctgt aaatgatatg gtttcacttt gtgacagatt tgaaaagaag
    25141 attcatgact tagaattaac tagaatggta tcgcttcaaa tggctcctca gataagattg
    25201 gttcaaaata gtaataatct gatggcagaa aagattcaat caactatagt aaataccatt
    25261 ccattatgga aaaatcaaat agtgcttgct cttggaattt ctcacttaaa tcaagctatg
    25321 aaagcacagc gtgaagttag taatatgaca aatgagcttt tattaaagaa tgcagaaact
    25381 ttgaagatgg gaacaataga aacagcaaaa gaatcagaaa gaggaattgt agatatagac
    25441 acaattaaga aaactaatca atctctaata tctactattg atgaggtagt taaaatacaa
    25501 catgatggta gaataaagag acaagaagca gaagttgaat tatcaaaaat agaaaatgaa
    25561 cttaaaagta agcttcttga atttacagtt aaataaaaat tatactaata taaaaagaga
    25621 ctgtctcaaa atagagataa gtctcttttt cattgtttaa atgattaaag tatatttata
    25681 ttataaattt ctattatctt ttaacataac atcatgagct ccaccttcaa ctatactagt
    25741 agaagataca agtgtaagtt tagcattttt ttgaagttca ggaatgctaa gtgctccaca
    25801 gttacacata gtagaacgaa ctttacttaa agaaagagtt acattgtctt tcaaagaacc
    25861 agcataaggc acataagagt caacaccttc ttcaaaagaa agtttcttat ctccaccaag
    25921 gtcatatctt tgccagtttc tagcacgagc agaaccttct ccccaatact ctttcatata
    25981 actaccattt atattaactt tgttagttgg actctcatca aaacgagaga agtatctacc
    26041 aagcattata aaatctgcac ccatagctaa agctaaagtt atatgatggt cataaacgat
    26101 accaccatca gagcaaattg gaatataaat acctgttttt tcaaagtatt cgtctctagc
    26161 ttgagcaact tcaattatag aagtagcttg tccacgtcct ataccttttt gttcacgagt
    26221 tatacatata gaaccaccac caataccaac tttaacgaag tcagcaccag cttcagctag
    26281 gtaaaggaat ccttcgcggt caactacatt tccagctcct accttaactg tatcaccata
    26341 tttttcacga atgaaatcaa gtgttatttt ttgccactca gaaaaacctt ctgaagaatc
    26401 tatacataaa acatcagcac cagcttcaac taaagcagga acacgttcag caaagtctct
    26461 agtattaata ccagctccaa ctacatatct cttagaagaa tctaaaagtt caagtggatt
    26521 ttctttatga gaagagtaat ctttacggaa taccatatac ataagattac cattatcatc
    26581 aagtacagga agagaattaa gtttgtggtc ccatataata ttgtttgctt cctttaaagt
    26641 aacatctttg ttagcataaa ctatagatga cataggagtc ataaattcac taactttagt
    26701 atctaaatcc atacgactta tacgataatc acgacttgca acaataccca ataatttacc
    26761 agttgaagtt ccatcttcag taacagctac tgtagaatgt ccagtttttt cttttaagtc
    26821 aagtatgtct tttaatgtgt tatcaggttt aatatttgaa tcacttacaa caaaaccagc
    26881 tttgtgggat ttaacacgtg ctaccatagc agcttcattt tcaatagttt gagaaccgta
    26941 tataaatgaa ataccacctt ctttagcaag tgcaatagcc attttgtcat cagaaacaga
    27001 ttgcataata gcagaagtca aaggtatatt catataaata tctgcttcct cgcccttttt
    27061 aaatttagta acaggtgttt ttaaacttac attagcaggt atacaatcag tagaagagaa
    27121 acctggtaca agaaggtatt cactgaaagt atgtgatggt gtatcaaaat aaaacgccat
    27181 tttttaatca ctccttatat ttttatactt tttataattg atagtattaa ttataactta
    27241 ttctttaatt ttattaaagt attttgtcaa atattgagat ttttgaaaaa tttaaactat
    27301 tttaattaaa tttgtttaat atatattgcc ttatagggta aagttgtata ttaaataatg
    27361 tttagtgttt aatagtagct aatttaaaat taatctttag tgattcatat aattacattt
    27421 gtatagttta cattgataat attactagag agtgcataaa cctcaaaaat acagataaat
    27481 taaatctata ttagtatatc caaaagtaca tttaataaat ttatttaact gaattattat
    27541 tttcatttct attaaattta aatattttat aaaaatagta ttatttacaa aatcatatac
    27601 tccaaaatac tacataaata aaattaaaca aaaactaaaa tgacaaatgt cattatctaa
    27661 agtgacatag aaacggtttc ttaaagttgt tactataaaa tataaactct ataataaaag
    27721 tataacaaaa gggaatcata tcgatataga atataattca agaggaggta aaattatgag
    27781 tagttctaat caatcgacaa taaagacatc tatacagcga gtagggaaat ttttaagtgg
    27841 tatggtaatg ccaaatatag gagcttttat agcatggggt cttataacag cattatttat
    27901 accaacagga tggatgccta atgaaaaatt gagtactata ggtgacccaa tgataaaata
    27961 ccttttacca ctattgatag cttatactgg tggtaaagca atagcaggac aaagaggtgg
    28021 cgtaataggt gcagcagcag ctatgggagt aattgttggt gcagatatac caatgtttat
    28081 tggagcaatg ataatgggac catttgcagg atgggtaatt aagaagtttg ataaatttgt
    28141 agatggaaag ataccaacag gatttgaaat gttagtaaat aacttttcaa taggtataat
    28201 aggtatgctt cttgcaatac taggatttta tgcaattgga ccagcaatag tagcagggac
    28261 agctcttata gaatctggag ttcaatttat agttagcaaa agtttattgc ctctagtttc
    28321 agtatttata gaacctggaa aagtattatt cttaaacaat gcaataaatc atggtatatt
    28381 agggcctata ggtatagctg aggcaaaaga agcaggtaaa tctataatgt tcttactaga
    28441 aagtaaccct ggaccaggtt taggagttct attagcatat tggatgttta gtagaggaag
    28501 tgtaaaacaa tcagcaccag gagctgtaat aatacatttc tttggtggta tccatgaaat
    28561 ttacttccca tatattttaa tgaatccagt tttaatattg gctacaatgg caggtggagc
    28621 agcaggaata ttgacttttt caatattagg agctgggctt gtagcagcac catcaccagg
    28681 aagtatattt gcacttatgg cactagctcc aaagggagga ttacttccag tattagcagg
    28741 agttgcagtg gcaacagttg tatcattctt agtggcagca ccatttgtta agagagcttc
    28801 agcaaatcaa agtgaagaag acagtacttc tttagaagaa gctaaagcaa aaatgagtga
    28861 tatgaaatct gcaagtaaga actctgagaa aaatattgag gaaaaacagc ttgaagtaaa
    28921 tgagattaag aagattgtat ttgcatgtga tgcaggaatg ggttcgagtg caatgggagc
    28981 ttccagattt aaaaatagaa taaaaaactt agacttaaat atagaaatta caaattcatc
    29041 tgttgataat cttccagatg atacacaaat agtagtaact cacaatacat tagttgaaag
    29101 agttgctaag aataattcaa gtgttgagat agtttcaata aacaattttt taaatgaccc
    29161 aaatttagat acattgttta aaagattaga atctaaataa aaataaaaag taccactaat
    29221 taggagtgga taatttgaaa aaaaagaaga ttacatctag gcaaaaaaag ataatactaa
    29281 tgatagtagc aaattcaaag aaaaacattc caataactat ttctgagatt gcagggactt
    29341 tagagttaag ttcaagaact gtacttagag atatgtctgg aattgaaaaa tggtttgatg
    29401 aaaatgattt taactttgta aaaaaaccag gagtgggatt gattttagag gaaaatatag
    29461 aaaatcaaaa ctttataatt gagttgttag aagaagaaaa aatagaaaaa gaatacagca
    29521 aggaagaaag aaatttaatt atattatcaa agctactagt ttctaacgag ccagtaaaat
    29581 cctattattt tgcaaaaata ttaaaggtat ctgaatgggt actgaataat gattttgcct
    29641 tagcaagtaa atggttggaa agatttgata ttgagcttgt tagaaaacca gggctcggtg
    29701 tctacttaaa aggtcaagaa aaaaacttta gagaggcata tgtaaatctt atatacgatt
    29761 cattcaatga aaaagaaata ctagatatgg tcagaaatat aagtgaaaat atacaaacag
    29821 ataaagcaat agaaatttta agtgaaaata gacttttaaa tttaatggac aggtgcataa
    29881 taagaaaagt tgaagctaca ttaacaaaga agctttcaga tttagatgta aatttagctg
    29941 atagtgcata tatagggctt gtagttcata tttcattagc cttacagagg ataaaaaatg
    30001 gtgaaaatat aactatggat aaagagtttc taaaagaatt atctataaca gaagagttta
    30061 aattagctaa agaaatcgta aaaggtatgg aaacagattt taacatggat ataccagtag
    30121 atgaggttgg atatataact atgcatataa gaggtgcaaa gcaaaggtca tcttcaaacc
    30181 ataaggcttt aaacttagat gatatagaaa taatggaaat tacaaataag atgatagatt
    30241 tggcggaaga tgaatttaaa ataagtttaa aaaatgacga gaggttattt aaagacctag
    30301 caaatcactt agggccttct ataaataggt tgaatatggg attagaaata agaaatcctc
    30361 ttttagatga aataaaaagc aagtattcat atgcctatga tgtagttgaa aagatttcta
    30421 gaattataaa agataaacta aatataaatt caatacctga atcagaaata ggatatattg
    30481 caatgcactt tgcatcagcc atagaaaaaa atcttatgat gaatactaat ataaatattg
    30541 ttgtggcatg tcccactgga ataggtactt caagattctt atcaactaag attgaaaata
    30601 aatttcctaa tttaaatata cttgaaacca tatcagctat aaacatagat gaagagtatt
    30661 taaaggaaaa agatgttgat ttaatagtgt ctacagttga gttaaataca agtttaaatt
    30721 atatatgtgt aggtccattt atgagtttag atgatgaaca gataatcaag gaaaaaataa
    30781 aatctatagc tcaaaataaa ttaatcaatt taaatgcgaa aaatgatact aagagtaaga
    30841 ataaagtata tgaacagata acagaaagta tgaatatagg aaaagatatt cttcaatttt
    30901 tagaggaaat tagatttgaa aaatttgaaa gtaaagattt aagtgagctt attgaagact
    30961 catcaaaaat atttgcaaaa tctagtgaag atataatctc tataaaagaa tcgttaaaag
    31021 aaagattaaa aatttcaatt ccatatatag aagaatcaaa aattctattg ttacattgta
    31081 tgagtgaaag aatagatatt atgaagttat caataataaa actagagaat agaatcgtgc
    31141 tagattcaaa tgaagaaatc gacaatgtag tttttatgtt acttcctaaa aattcaccaa
    31201 gttatcaaag acaaataatg agtgaaataa gtggttcatt aatagacaat ataattttta
    31261 ctaataagat aaataaattc tctatagaag agatggcatt agaggtaaaa gatatagttt
    31321 ttaattttta tacaaacaga ttaaaagcat ttatagataa ataaaattaa tattaaaaag
    31381 gaaggttgat actatgagta aagtattgaa tgagaataat atatttttag ggttagatag
    31441 tgtttcaaag gaagaagcta taactttagc aggaagaaaa ttagttgaaa atggatatgt
    31501 aaaagaagaa tatataccag caatgttaga aagagaaaaa gtaatgacta catatatggg
    31561 aatgggagtt gctattccac atggtgtaaa tgaagcaaaa aaagaaatat tatcttctgg
    31621 aatagttata ttacaattcc ctaatggtat agattttgat ggagaaaaag catatttatt
    31681 aataggaata gctggtgttg gagatgaaca tttagaaata ctatctaata tagcagtagt
    31741 tttagatgat gacttaacag aaagactaaa aaattcaaat gacaaacaag cttttatgga
    31801 agcttttgcc aactaatctt aggagggatt taaaatgaaa aaggcaattc agtttggagc
    31861 aggaaatatt ggaagaggtt ttattggagg cttgttagtt aagtcgggtt atcatgtagt
    31921 atttgctgat gtaaatgagg agatattaaa ctcaataaac aaagataaaa agtatactat
    31981 acacataaga gatgtagaat gtatagatga agtaattgat aatataagtg cggtttcatc
    32041 tataaaagaa gaaataatag atgagattgt acaggctgaa ataataacta cagcagttgg
    32101 acctttagtt ttgactaaaa tagcctctac aattgcaaaa ggtataaagg ctagaaaaga
    32161 aaagggatta acaagtaatc taaatataat agcatgtgaa aatgcaatat acgcaagttc
    32221 ttctttaaaa gaagaggtac ttaagtattt aaataaggaa gaagtagaat atttagagat
    32281 gtatgttggt tttccaaact gttcagttga tagaatagtt cctcctggta aaaacgaaaa
    32341 tcctcttgat gtaacagttg aaaattttta tgagtggaat gttgaaaaac aaggatttaa
    32401 aggggaaata ccaactatag ttggtatgaa tttagctgat aatcttatgg cttatataga
    32461 acgtaaattg tttacactga atacaggtca tgctataaca gcttatatag gatatttaaa
    32521 aggatataag actatagaag agagtataaa agataaattt atctgtgata tagtcaaaag
    32581 tgcaatggta gaaagtggag aaggattgat taaaaaatac aattttgact cagaagtgca
    32641 ctataaatat attgataaaa ttttaaatag atttaaaaat ccatatctta atgatgatgt
    32701 attaagagtc ggaagggaac cattaagaaa actaagtgat aaagatagat tgattaaacc
    32761 tttaatgaca gcaaaatcat atggattatc agttgataat ttgattcttg gaataggtgc
    32821 agcgcttcat tataataata gtgaagatac tcaaagtgta gaattacaag agttgataaa
    32881 gtctattgga gtaaaaaaag ccgttgctaa aatagctaat ataagcaatg acgaagaact
    32941 tttaaacaat atcgaaaaaa gttatatatt tatgaaaaat ttataaattt acttgaaaaa
    33001 aagtagttct gataaagtaa ctataatata ttattctaca cagtttgaat tatttgttga
    33061 ataatagaga attataatat ataattaaaa ggaataaatt aatatattta cctgtatatg
    33121 tttgataata tggttcaaat gtttctacta agctaccatt aatagcttga ctacaggagt
    33181 gaagaagata tagtttatac ttattttaaa ttatatatta agttaattac tttgaatttt
    33241 aagctcctgt ctatgatata gacaggagct tttttagtat tttaatataa aatacttaca
    33301 agaatagata gcaaaattag aacaaatata atttttttat taatataatt ttagaaaggg
    33361 ctaggtggaa gttcaatgaa ggctttacaa gaaaagatat tgagagaagg cagtgtttca
    33421 ggaaatgata tactaaaagt tgatagtttt ttaaaccatc aaattgatgt agctttttta
    33481 aatgaaatag gaagagaatt taaagagaga tttaaaggag aaaaggtgga taagatattt
    33541 acaatagagg cttcaggaat agctatagca tcaattgttt cacagtattt tgataatgca
    33601 cctgtagtat ttgcaaaaaa atcagaatct aaaaatttgg ataaagatgt atatgaaact
    33661 aatgtatatt catttactaa ggcaagagag tattcagtta aagtttctaa aaagtacata
    33721 aacaaaggcg aaaatatatt gatagtggat gattttttgg caaatggaag agctgctctt
    33781 ggacttaaag atttaattga gcaagcagaa gctaacttag ttggagtagg aattgtcatt
    33841 gaaaaaggat ttcaagctgg aggagcactt ttaaaggcta atgatgtaag attagagtct
    33901 ttggcagtag ttgaatctat agataatgga actgtaaaat ttaggtaatt tatgatatta
    33961 tatacttgtg tttagcacat attttaacta tgtaattatg gaggattttt tatgagattt
    34021 tttatagaca cagccaatat tgaagaaata aaagaagcaa atgatttagg tgtaatctgt
    34081 ggtgtaacta ctaatccgtc tctaatagct aaagaaggta gagattttat agaggtagtt
    34141 aaagaaataa gtgaaatagt agatggacca ataagtgcag aagtaataag tttagaacat
    34201 aaaggtatga tagaggaagc agaaaaatta tctaagatac ataaaaatat agttataaag
    34261 atacctatga cagcagaagg tctaaaagcg gtgaaagtac tatcaagtaa aggtataaag
    34321 actaatgtaa cgcttatatt ctctgctgga caagctcttt tagctgctag agctggagct
    34381 acatatgtaa gtccttttgt tggtagattg gatgatatat cacaaaatgg tcttgattta
    34441 attgaagaaa tcgttgatat atttagtgta aatagtattg aggctcaaat aatagttgct
    34501 agtgttagaa atccaataca tgttttacaa gctgctagaa tgggagcaga tatagcaaca
    34561 gttcctctta aagttataaa ccaaatgatt aagcatcctt tgacagacaa aggaatagat
    34621 agctttatga aggattggga aggtgcttct ttaaaattat aattataaac tacgctttat
    34681 aagtatatta aagaggttat tatggaaatt ctaagtaata aggctaggct attttgccta
    34741 gtcttatttt ggtgtataat atattgaaac atattattga aatagattaa ttttttaagt
    34801 gaggaggcga aaaatggata aaatattaag agagatacaa agagaagata aaaaaaatcc
    34861 atatactgac caagaacttg ctgaaatgtt aaatgtagct aggtctgaag tgatatcagt
    34921 taggaaaaaa aataatattt tagattcgag agaaagaaga aaaaaaatat taataaaaga
    34981 tatatcaaaa attgtaagtg aaaatcctca aatgtctgaa agaaaaataa ctgaaatatt
    35041 aatagaaaat ggatataata tatcgagaag tgcagtttca aaattattaa aagaggaaaa
    35101 tttaaatcag tcaaagattg ggattaagga taaagaccca gcatcatcag gaaagtatgt
    35161 gaaggtgagt aaagttgtag aagaagatgg atttgaagaa ttgataggta taaagggaag
    35221 tttgaaagaa aaggtaaatc tagctaagtc agcaataatg tatcctccta atggccttca
    35281 tactataata tatggagaaa caggagtagg taaaagtgaa ttagctacat gtatgtataa
    35341 atatgctata aaaaacaaca ttaaagaaga gaacagccca tttatagtgt ttaactgtgc
    35401 agactatgca gaaaatccta atctattgat agctcaactc tttggggtag taaaaggtgc
    35461 atatacagga gcagatgcaa atagagaagg tcttgtagaa caagctaata atggaatatt
    35521 gtttttagat gaaattcata gattgcctgc tgctgggcaa gaaatcttat tttcattaat
    35581 agatagagga gaatttagaa ggttaggaga aagtagttct aatagaaagg caaatgttct
    35641 cataataagt gctacaacag aaaatccaga ttctaattta cttcaaacat ttagaagacg
    35701 tataccaatg gtaataaatt tacctaatat gagtgaaaga ccaaaatctg aaagatatga
    35761 aataataata aagttttttg aaagagaagc aaagcgagta aacaagaatt ttataataac
    35821 taaagaagtt atgtgtgcac ttatgaatta taagtgtact ggaaatatag gtcagttaaa
    35881 gagtgatata caagtaactt gtgctagagc ttttagtaaa tcgatatttt ctagcgataa
    35941 agtaattata gatttagata gtttaaggga ttatataaaa agtggatatt atgattgtaa
    36001 ttgtaaaaat gaaaagtatt ctgagttttc agttgaggat atatatatag atattagtga
    36061 attggtgagt ggaaaaaata gaattttatc tgataatgag ataagcgaga tatataatta
    36121 tgcagaaaaa gagcttaagg ttttggaaag taagtgcttt tcagaagaag agttaaaaaa
    36181 tgcatttata gaaaagttag ataagaagtt taatgctatt aaaaacaata aaaatttatc
    36241 tgatagaaga agtcgaatag actggggagg acaattaaaa gagtctacac tggagataat
    36301 ggataaggta attgtattta ttaaggagca gtttaagcat gtaaatcaag gtctgtactt
    36361 ggctctagca attcatattg agcatgctat aggtaggata aaagatggaa aaactataat
    36421 aaatccgagt ttagataaaa taaaagcatc aatgccaatg gaatatgagt tgtcaagata
    36481 tataatgggt atagtagaag atttgacaca agtaacattt ccagaagatg agcttggata
    36541 tttggcttat tatataaata aattttgtta taatgaggaa agtataaaag ataaagttaa
    36601 agtagtaata gttacgcatg gtaaagttgg aattgaaatg tctaaagttg taaatcatat
    36661 tttaggtata gaatgtacac taggtataga aatagcactt acagattcac catcagaggg
    36721 gatagagcat gtattagaag aacttcaaaa gatagaagct agaaaaggta ttctagttct
    36781 aattgatatg ggttctttgg taattcttgg tgatgaggtt gaaaaaaggc ttggaattag
    36841 atgtaaaact gtaaatagag tagatacatt attggcaatg gaagcaggaa aacttgcaac
    36901 aattgaagga aaaagtttag atggtataat agctgattta aagaaaaata agaattatgc
    36961 tatgataaat acaaataaat ttagttacag aaaaaacgag tatggaaaga aaaatgtaat
    37021 cattacttta tgtttgagtg gtgtaggtac agctttaaat ttaaaagagc atatagaaaa
    37081 acaaattaag gaatatgaca ctttaattga agtaaagcca gttgcttttt taaataataa
    37141 tttagaagaa gagttaaatg atattgaatc tgaatataat attattgcta tctgtggaac
    37201 tattgatata gattataaaa acgtgccttt tatttcttat aatgaggtac ttggtaaaaa
    37261 tggtattaat aaagtattag agcatttaaa tagtaaacaa ccagctttaa atattgaaaa
    37321 ccagttgaat aatttaatac atgaagattt gattttatac aattttgaag gaatttcaaa
    37381 ggagtatata attgatactc ttgtttcaaa acttgaagaa ggtggatatg ttgactcaaa
    37441 gtatatatta agtgtatata aaagagaagc aatgggaagt gttgtaatgg cctctaaggt
    37501 agccgtgcca catggacttc cagaaaacgt tatgaagcct gcaatagcaa tcgcgaggtt
    37561 gaataaacct atagtatggg acaataaatt tatggtggat ttggtggtac tactggcact
    37621 taaagaaaat aataagaaag aaatacgaag cttattttca aagattaatg accagcatac
    37681 tttagaagtt ttattaaata ctgaagataa aaatgaaata aaagaacttt taagctaatt
    37741 attagtaccg agaagaaaga caaaaaaatt tgctttagta taaaatttaa aactactcaa
    37801 ttgctaagta gttaattact ctatataagg ctag
//
LOCUS       DAMJYR010000028        37649 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.028, whole genome shotgun sequence.
ACCESSION   DAMJYR010000028 DAMJYR010000000
VERSION     DAMJYR010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 37649)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37649)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37649
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.028"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            130..528
                     /locus_tag="PC471_001352"
     CDS             130..528
                     /locus_tag="PC471_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF2788488.1"
                     /translation="MDTLGKRIAYLRNSKKLTQRKLMDILKFENLGKYETGDRKPNCD
                     ILMSIADYFNVTTDWLLYGKEKVNVNSSVKEDKEDYLHVTNDEMTILNLYRQLNERDK
                     IKIEGILELKISEYKDLKKHSSNNNEDKMV"
     gene            complement(745..954)
                     /locus_tag="PC471_001353"
     CDS             complement(745..954)
                     /locus_tag="PC471_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2788489.1"
                     /translation="MCKLDSDGVIECCRAIDDFITALSNIKSLNMERLNTLTKYSSTC
                     SILLKEGNYEGCTIVYRKMLEELKT"
     gene            complement(986..1195)
                     /locus_tag="PC471_001354"
     CDS             complement(986..1195)
                     /locus_tag="PC471_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2788490.1"
                     /translation="MDARKKWIPFLGVQVKQRLIELNMTQRELAKKIGVNENYLSAIL
                     NGRRTGKKYKSSIYQLLNIEYSEDD"
     gene            1494..1913
                     /locus_tag="PC471_001355"
     CDS             1494..1913
                     /locus_tag="PC471_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2788491.1"
                     /translation="MDTLGERIVYLRKAKNLKQYELEEMLGCDNLSKFERNIRKPNYE
                     ILKSIAEIFNVSVDWLLNGDNLSHKSDLICDSSSNYPLNSINSNEIKLLNNFRKLNDY
                     DKAKIEGMIELKLHEYEKEKDLGKIEYNKNKDEKIDK"
     gene            2141..2596
                     /locus_tag="PC471_001356"
     CDS             2141..2596
                     /locus_tag="PC471_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2788492.1"
                     /translation="MGLRDKFAQSFARSKTMSGPEKKANEIMGKLLLKKAILPIVLMF
                     VIIIAGAMLKINSWVTLGINLVIAVGAFFYIRNSSKKYQNFKPYVGNLISLEKKGKKE
                     YVAIIKQGKLPVKLQIAYGGEDLEHVKKNQMVQISYNPDAKIAILVNRQ"
     gene            complement(2817..3077)
                     /locus_tag="PC471_001357"
     CDS             complement(2817..3077)
                     /locus_tag="PC471_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2788493.1"
                     /translation="MDKLITELSSLGAIGILCALLFKNTMQEKKEDRDMYKKTVENFI
                     ELSTQQQEINKNILVQMGIMKTDVEEIKEDVTGIKGMLQNGV"
     gene            complement(3340..3693)
                     /locus_tag="PC471_001358"
     CDS             complement(3340..3693)
                     /locus_tag="PC471_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2788494.1"
                     /translation="MFKFDKNKIEQIKQGRKVEMQYKDISDISIGQVKQDDDITNNFI
                     ANAEIYEMLLNQSSVNEASNISSFSVRKSGGESGMVEVYVALILRGKKTIEEVPAVIR
                     EQVRIRCKELEIPVE"
     gene            complement(3710..9031)
                     /locus_tag="PC471_001359"
     CDS             complement(3710..9031)
                     /locus_tag="PC471_001359"
                     /inference="COORDINATES: protein
                     motif:HMM:NF012632.1,HMM:NF024930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2788495.1"
                     /translation="MKRTKLLQRGNFFGDKNMVVDEFDEGYDNYDFINFFTGCCNYTF
                     GLKNNNILYGCGDNSNFQLGLGEDNTTRKLFTKIPNISTNIKKVACGESHAVILTSDG
                     ELLVAGINTDGQMGLGLEKVGKTVSTFEKVPEIKGVKDIACGLQSTYLLYNDGTLYVA
                     GNNLYGQLGLGTNGASANVNTFIKVDVDNVKAVFSYNKSAFIIKNDNKCYSTGFNNQG
                     QLGLGDKNNRDLFSLVSINDVKTIACGSEHTVLMTYNNDIYGCGKEKCFGNALQSSLF
                     TKIEEVNIKTIACGNGNTMLIDNKGILKVAGNNDIYQLGIANYSENIDNSFIDLKNIV
                     AKNIFIGLSHSILIDSNNDSYCTGDNTYGQLGSFFDDMHIVEFKKMDSEKYSYSNYIN
                     LIKSEDKLTLLKEEMEIKDIELPLDIHSVRDVVFSPYCTLVILGNGDVYGLGNNRYKG
                     MGSDLPSQLNELTKLSISNVKSIVASKNISGGIFYIKNDDTCYYSGPNSNSIAGVLPS
                     NSDIFKKISIDNVKKVVINTDLSNWFSLIVTNNKQIYTSGKSSSYVNGLSNKSASQYT
                     EIGVSNVTDAYSSYNATFIVADEKKVYATGINTNYLLGVNPNDGVNINLTLINNWFYV
                     NPSGASYSTNSCTNDITKINNIIIYEYMAILCTNYGSFLTGYHGTLWTKPSDSTYRVQ
                     QQGITHAGYISNYIYNYYPTRCTQSSYSTNFAYLYYGESTSNLKNVNPDNLLISGGSS
                     YIHQYGRNYLNNQSSNNIAASNINSGPITSDKAIFLYKALLYLSSNTLYGFGNISESA
                     KELDVSDTQDGYNATNYKKVMKNIKNIFIPPYDLSRDKTRFAILTDKSLFICGYNSKG
                     THGISVNSSLNLNNKINYNKKNSSSEISSNIQEIYSHSKSTYLLTNNNMLYSVGLNDV
                     GQLGVGDEINRKVFTKINIDNIKSINVNRFTDNSKHVFAIKNDNTCYAVGLNNSGQLG
                     IGDNVNRNVFTKINVENVKYVAVYGNTSLLLTNDGLLYGAGNNGKGQLGLGDTTSRNI
                     FTRIPINGVRDVYICNDVSIIVKNDNTCYVCGFVNGYFGFTEGSISTFTKINIENVKS
                     VVTAGSEATFFITNDNVIYTTGKKERVFFSTETNDIKGIRVINNIINAKKIVVNGYTS
                     AILTNDNKLFVGGLSGYGSIANNNNTNSVEDVKDVFVTANNTLYIDNNNNLISSGRDT
                     YGISDESYRDMSVPYYKVSIKKDVDTVFSSYNTIFIKDIYGKFYSSTGDNRYNHLGIH
                     HRYDNDKNEALEGSLHSYFKTDNTSDKIVFNKKNEKLVMFNDKYIKTNNKYINYKNIF
                     KDNFKYTSIILPFEVSDIDISKTHSLAVAKDGKLYGIGSNSYKEINQTLEDIELLTLT
                     EVNISDVKKVACGDNYSYIIKTDNTLWSYGKNTEYQLGVGHNNDVRELQKVTGLPSVK
                     DISIYNSMTLVLTNEGELYAQGYNTNGLFGLGESEKDKIIRTFTKVLTNVKEIKSHND
                     DHILVIKNDNSLWITGKNKSMYKISISITDLYEFTKIPIPEHLNDILDIELSDDTIYM
                     ITKVDTSKASIEIVEKSISQVRVVVQDPNNVIEKLEMFINDELISTKTNLEINSIIFE
                     IPQNKIVLGENKILIKASSPTGDLYSSMFIFKSETGLKVKKDSILMINNKVYSIINIT
                     ENNTDLIVTLNEGLKDDMMENNPIYQLINKTKVQVKINKSDLFKDMKLVEIKKSDSSY
                     QEIYELEEANIKSAQPKIIVEKGDKWTAIKRPSMIFRYDAESNEPQA"
     gene            complement(9048..10031)
                     /locus_tag="PC471_001360"
     CDS             complement(9048..10031)
                     /locus_tag="PC471_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDF2788496.1"
                     /translation="MEEKFYIILTKIGREKIANATALGELVGLTKFQVGDSNGEYYEP
                     TEEQTALKNVVWEGNINSLRIDEKNPNWIVIETILPGTVGGFMIREAAVLDNENNIIA
                     IGKYPETYKPRAEDGSIKDLVVKMILQLSNTSNVTLEVDPTLVFVTQKDIQDLDDKVD
                     KNIKEIKVKIGEELLSTEAKNLSGAINEVVEKIKNISIDDVIGGQIQTELSVLKNSYN
                     KLSEKVLDILIYLELESEIDVDEAGYWYDTLTNAKNIIAIEGLKLDLNRKCITGELGS
                     VTFKNVVLPFNANRVRYIHEMDNNFVETKSNRAYSIGQTDITLNKYSYEIR"
     gene            complement(10032..10730)
                     /locus_tag="PC471_001361"
     CDS             complement(10032..10730)
                     /locus_tag="PC471_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2313 domain-containing protein"
                     /protein_id="HDF2788497.1"
                     /translation="MKLIDKLPSFDRNYIVEEIQGAYDTELNILKEDIDDTFNQLFVD
                     TATWGLDMWEDILCIEKKELDFDTRRSNIKAKMRSRGTSTIEVIKSICEAYTKSETDI
                     KVYSDEFTFVLSFIANNCDYKTLLDCSDMIERVKPAHLLHYLEPIILDKSMVYCGGGM
                     VCSEEVKVHPYFEPIIKCSAVVNCGAGMLSREEIKVYPLSIKCIENNCKINIAIANDT
                     GVENVVVYPKSEVV"
     gene            complement(10727..11779)
                     /locus_tag="PC471_001362"
     CDS             complement(10727..11779)
                     /locus_tag="PC471_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HDF2788498.1"
                     /translation="MYSDQTYEVIKNRTLENINLDIYKGEGSFLNNMVSGNNLELSKI
                     YLELSKIHKMAFIQDTYNQFLDKRVNEFGVYRKLGTESNGEVEFIGEKGTVINNGTII
                     SYRDLLFVVIKDVTIGSEEGDNSPVQALEVGKKYNLPTNCEFKLVDNISGVTKITNTR
                     SFEGGTDIETDEELKERFYKIQRNQATSGNKAHYEEWALEVDGVYNVKVYPRWDGPGT
                     VKVLIFGKNNQAVDTETIERCQQHIDEEKPIGPTITVVTPLPIEINISAVMKLEDGYT
                     LDNVKESFLESINTYFRDIRGEIIYTKVMGILINTTGVHDLSNLLINGSTDNITINED
                     KIPSVTTVNFSEVENQ"
     gene            complement(11772..12200)
                     /locus_tag="PC471_001363"
     CDS             complement(11772..12200)
                     /locus_tag="PC471_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2634 domain-containing protein"
                     /protein_id="HDF2788499.1"
                     /translation="MSTIFPFIGVPEDYILPKTEELPIFREVAWDFEKDEPILEKGDF
                     KIIEKKEALKVWIYKCIKTNRYEHEIYSLEYGTELSELIGQKYTKGLTESEASRFIKE
                     ALLINPYILEVNVKSANFNRDVLSANVKVSTIYGEVEINV"
     gene            complement(12200..12526)
                     /locus_tag="PC471_001364"
     CDS             complement(12200..12526)
                     /locus_tag="PC471_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2577 family protein"
                     /protein_id="HDF2788500.1"
                     /translation="MANPINEFIGIIREEGKYHNQPSFFIGKIKSKLPDLKIETNNII
                     LEKEDILIDSWMIDRQLETFDTETNQEHQHEVKNPFIDNFESGDMVIMFRIGEKFAVV
                     SKLVSL"
     gene            complement(12542..14071)
                     /locus_tag="PC471_001365"
     CDS             complement(12542..14071)
                     /locus_tag="PC471_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HDF2788501.1"
                     /translation="MIINRSKDSSSNEISFVSKDMGFLLTQSEVSYNFKDKLVEDIAK
                     QVFAENRLSVGTIAKTNVKYTKMFIGVNGYDTIMSAYTEASKKTKKKYMIEANLDKFN
                     VIEKGTVTLNVMFEEGFNIINTTFSESMENVKNKVIVVDQYGSKISEKIDNEIFKEVN
                     VIMQKVIQQQENQDVDIDSEFNGIEKSCSLKGYGDVSCITGRGVKVKDSYTKLVGLFY
                     IDTDKHTWQNGEYQIELELNFQNLMDEKSAGQDEPKEESNLGGEDYAGGKEFTAEFTA
                     YCPRKEEGGDTDCRKKKLDPSKKTCAAPMVGKYEQTYYTKEFLNKHPLLNYGDEIQVI
                     TGVSGRDGVYKVNDVGPAITIEKNGTYHIDILFGNVEEASKFGRRKGKIIIGGYSGNV
                     SDKAKIVISEAKKHLGKPYKWGGNGPSSFDCSGLMVYCFKKVNVSLPRTSNQQSKKGK
                     KVEQKNLQAGDLVFFHNPVSHVGLYIGNGEFLHAPQKGDVVKISKLSSRRDFNTARRV
                     L"
     gene            complement(14353..14775)
                     /locus_tag="PC471_001366"
     CDS             complement(14353..14775)
                     /locus_tag="PC471_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2788502.1"
                     /translation="MEMWLRQAEDRFRFPVFPSSFSINGKAAVNSSSILKIGEIATFG
                     GVALKSISISSFFPNKDYTFCDYTGFPSPYDCVNKIEKWMKEGFILRFTITETNINME
                     VIIEGFSYEERDGTRDVYFTLDLKEYKRIKIPKVTPKQ"
     gene            complement(14775..17228)
                     /locus_tag="PC471_001367"
                     /pseudo
     CDS             complement(14775..17228)
                     /locus_tag="PC471_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889141.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(17228..17395)
                     /locus_tag="PC471_001368"
     CDS             complement(17228..17395)
                     /locus_tag="PC471_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2788503.1"
                     /translation="MYYYWKKKGIRPSLFYAMDKGELKLIEAFFALEIEEEVEKMKHG
                     YGVCPLTGGGM"
     gene            complement(17422..17868)
                     /locus_tag="PC471_001369"
     CDS             complement(17422..17868)
                     /locus_tag="PC471_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HDF2788504.1"
                     /translation="MSENGLSKNINIVDLLLNSDTENLERPSTIVELKRLSTIFGQEF
                     KVMCRALTISKDEEIQNTCLKIDENMKTDIDLPEMQMLTIIEGVCDLDGKLLFKNKEL
                     MDKFKAPTPKELARKLLLPGEITNLYRILQDVMGYGKNAVIEEVKN"
     gene            complement(17994..18422)
                     /locus_tag="PC471_001370"
     CDS             complement(17994..18422)
                     /locus_tag="PC471_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HDF2788505.1"
                     /translation="MANMEARNVMSGTWGELWLDGNKVAEVKKFQAKMEFTKEDIIIA
                     GQMGTDTKYIGYKGKGSITLYHVSSRMHKLIGEKIKRGSEPRFVAISKLNDPDSYGAE
                     RIAVKNIAFDDLTLADWEVGVKGEIEAPFTFTEYDFLDII"
     gene            complement(18436..19500)
                     /locus_tag="PC471_001371"
     CDS             complement(18436..19500)
                     /locus_tag="PC471_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HDF2788506.1"
                     /translation="MAIGLPSINISFKELATTVKERSARGIIAMVLKDAKALGLNEIH
                     EKEDIPVDLSAENKEYINLALMGNVNTPNKLLVYVIEGEADIQTALDFLETKEFNYLC
                     MPKAVEADKTAIKNWIIKLRDIDKVKVKAVLGKVVGNHEGIINFTTEDVLVGEKKYSV
                     DEFTSRVAGLIAGTPLSQSVTYTKLSDVVDIPKMTKVDAESKVNKGELILIKEAGAIR
                     IARGVNSLTELTAEKGEMFQKIKIVDTLDIIHSDIRKVIIDDYIGKVTNSYDNKCLLI
                     VAIKSYLEELEKSALIESDSTVEIDFEAQKSYLKSKGVDLSYMTLQEIKEANTGSKVF
                     LKAKIKVLDAMEDIDLSIEI"
     gene            complement(19505..19948)
                     /locus_tag="PC471_001372"
     CDS             complement(19505..19948)
                     /locus_tag="PC471_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2788507.1"
                     /translation="MLKYKEILETIIEILKKNFTESIFIDDESVQGSEGSCFFVSILS
                     VICTPVMLNTNNKDIVISIKYLPKPQSKSIRMYEVSDELNKLFNRNIKVTDRKLNITK
                     LEQSIKKEESIYVLNFTFTLNYLDSVYEEDVVYENMEEINLNLGE"
     gene            complement(20197..20637)
                     /locus_tag="PC471_001373"
     CDS             complement(20197..20637)
                     /locus_tag="PC471_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2788508.1"
                     /translation="MENKKDILFKETDKRLHNYKYLDIKIKNINLDIKRCENEYSGCG
                     AMVYTEKTSNTYNISSSVENEVLKREERLRKLKMEKEDIEIEKEKIENALTCLNDIEM
                     EFFNLFYNSKTKNNMTYISMKLHLDRTSCYNLKKKMIFKLSEIL"
     gene            complement(20742..20939)
                     /locus_tag="PC471_001374"
     CDS             complement(20742..20939)
                     /locus_tag="PC471_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2788509.1"
                     /translation="MFKNNLKYYRKCKGMTQIQLARKAGITNDYISQIERGIKNPGLL
                     MAKKISSILEQNIEEVFFIQL"
     gene            21108..21428
                     /locus_tag="PC471_001375"
     CDS             21108..21428
                     /locus_tag="PC471_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HDF2788510.1"
                     /translation="MFAKRLRELRKEFGLTQRELGEKVGVSQRVLGYYETENRFPDEH
                     ILNKLADVFNVSVDYLLGRTLVKENIDTVAAHRKNPHEELPEEAQEQLNDYIEFLLNK
                     YKKK"
     gene            21510..22016
                     /locus_tag="PC471_001376"
     CDS             21510..22016
                     /locus_tag="PC471_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2788511.1"
                     /translation="MNNLDKLFELASQEEIIIHYTTYIAGDLEGLYINKHGIKIISLL
                     SNLKQNSKKLTSILAEELGHHFTSLGYYVSSYNDYYTKIIIDKCENKALKWACEFLIT
                     EEDIINIINSGITCVYEMADILNVDITFFQKRLEFLSLKKQSLQLGNNKYLILTNLPY
                     FYIFDPIS"
     gene            22717..23472
                     /locus_tag="PC471_001377"
     CDS             22717..23472
                     /locus_tag="PC471_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HDF2788512.1"
                     /translation="MEKKVLATVGEKEITNIDIENALKSLDPYQAMQFKTEEGKKHLL
                     NDLVNQELFFLDAKEEKLDEEEIFKLEMKKIEENVLKQFAINKVLSSVNVTEDEKVKF
                     FEANKSSFSKPESATAKHILVDSDEKAKEILAQIKSEEISFEDAALKHSSCPSKDMGG
                     DLGTFGRGQMVPEFEEAVFSMAKGEVSEPVKTQFGYHIIKLEDLQESTESTFDEVKAE
                     VEKSLLYQKQNEVYGNKINALNAKYGNLVKYND"
     gene            complement(23615..24043)
                     /locus_tag="PC471_001378"
     CDS             complement(23615..24043)
                     /locus_tag="PC471_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HDF2788513.1"
                     /translation="MNILFFLTPKSEVAYIYEDYTIRQALEKMEYHKYSAIPIISKDG
                     KYVGTITEGDFLWTLKNDLNLDLKGLEDVPVTDINRKMDNSPVSINADIEDLVIKSLN
                     QNFIPVIDDQDTFIGIIKRRDVIGYCYEIIRGYKNLANDN"
     gene            complement(24464..24664)
                     /locus_tag="PC471_001379"
     CDS             complement(24464..24664)
                     /locus_tag="PC471_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HDF2788514.1"
                     /translation="MKTGVVKWFNNEKGFGFISVEGEGDVFVHFSAITGDGYKTLEEG
                     QSVEFEVVDGAKGPQAANVVRL"
     gene            complement(24847..26016)
                     /locus_tag="PC471_001380"
     CDS             complement(24847..26016)
                     /locus_tag="PC471_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase domain-containing protein"
                     /protein_id="HDF2788515.1"
                     /translation="MGGVALKILKNKNKYIKGIMAFVFLSLATTAGVFLINDLSMKNM
                     EEKVMESANVEGDDVKDQYKLLLENLFDYRNKAILEKNENILKGLYETDKKFGLWAYE
                     NEVKKMKYLENWSSKQGVKFKDIKTRVKIKKIKEKEKDLYGIICSVSTEYKYSYENQK
                     DKENAFRIGTYHYLNVKIIDNQYVITKEWYTDPFADSLNLENIKSDDIRNYIGQQDGV
                     ELQLTDEQKKAIDYAHKYCGAAADEEHGMKFNPNYRDYNPEGGDCANFASQIMFESGR
                     FKKNSIWNYSERAGTKAWVNAQAFKNYALYSGRGSLIAKGSYEEVYKEAYNLRPGDFV
                     GYEKKGRITHVSTVTGLDSRGYPLVTCHNTDRMLVPWDLGWSDKSIRFHIIKINY"
     gene            26252..27073
                     /locus_tag="PC471_001381"
     CDS             26252..27073
                     /locus_tag="PC471_001381"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine kinase"
                     /protein_id="HDF2788516.1"
                     /translation="MQKKVAAINDLSGIGKCSLSVAIPILSALKVQCCPFPTAILSSQ
                     TGYPEFTFLDFTDEMVKYSNVWKNLKVNFDSIYSGFLGSKHQIEIVANFINDYPNAFI
                     VVDPVMGDNGVMYPIFTEEMRQEIKELVKHSDLTTPNLTEACFLTGNDYTKSDYNRDE
                     LIYIAKSVSDLGPSKVVITGILEDDNILNLAYNRDNDHVFFTSVKYNNCSYSGTGDIF
                     TSILCGMLVNKHDLGVAVNTATDFIYKTINYTSQFDTDRNDGVMFENFLSDLTNI"
     gene            complement(27149..28537)
                     /locus_tag="PC471_001382"
     CDS             complement(27149..28537)
                     /locus_tag="PC471_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase"
                     /protein_id="HDF2788517.1"
                     /translation="MLGGKKSNNKKVEKLRTIFIKYLSLFFIMTISIVLLLMLSFSVL
                     LSSGVILPANYAEKQFNKYKEQIISSEKVTEDIIPSIYEYGVYTLDGNLISGTFNKKE
                     SKEVWNLMRDIEERHAYSESYIKFFKKDEVFIIKYKIVSEYSSPILRAYLPKPETLGM
                     IIFSIIFFIEIVILSKVFGKKFNIEMELLKNTTEKIEQQDLDFVVESSKIYEINNVLF
                     SMDKMKLALKDSLEKQWMLEENRKEQISALAHDIKTPLTIIHGNTDLLIEINENPELL
                     EYMEYISKGVTQIEKYINTLIDISKTENGYILNKEAISMSEFIKDVLVQIEALASTKN
                     LDVEFYKQDNLPKNITIDRELLFRAIMNIISNAIDYSPSQSKLYISVSVHNKYLKFVV
                     TDCGSGFSKADLSKATEQFYTGDLSRNSKSHYGMGLYIVNNIVQKHNGTLCIENSVKT
                     GGAMVTIEIPIV"
     gene            complement(28672..29430)
                     /locus_tag="PC471_001383"
     CDS             complement(28672..29430)
                     /locus_tag="PC471_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic immunity ABC transporter MutG family
                     permease subunit"
                     /protein_id="HDF2788518.1"
                     /translation="MRQLIRIFCSDLIKLKRNFIILMHICIALMGMGLFLWYYKVSGA
                     DNILKIVAYLQVIAIAFPLLSSVVCSMFVEQEYYAGNYKHMLTTSNPKYLTLISKYII
                     LVCLGFVATLVSVLGFKFGISKAYFTSSFYMISILILIGCNLFEYILHLFLSLRFGKG
                     TSIGVGIVEMLLSALLLTGLGSGIWQYFPCAWGVRFITIWSSFSSSKTVEYIKVESIK
                     AYQSTGLICGFVTILAFVILCIWFSKWEGKKSEE"
     gene            complement(29432..30148)
                     /locus_tag="PC471_001384"
     CDS             complement(29432..30148)
                     /locus_tag="PC471_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic immunity ABC transporter MutE/EpiE
                     family permease subunit"
                     /protein_id="HDF2788519.1"
                     /translation="MERFIRVEFQKMKGTFGMKLIWIVPVFCILLCGLGGGQNAAFNW
                     WYTTFLPGALTLISSLIVLKDEKSKYRGILPLPIEMKDVWTGKMIYCIICCFIASIIS
                     AIGVVFVGIIYKETISIGQNIIASILIILLFMWQIPLCMFLASKFGMFVTVLTNMFFT
                     IVGVAVFSTTEMWYMFPYSIPSRLMAVVLHILPNGLPVPEGSEFLNNAVILPGVLISI
                     VLFVLFALATRVWFNNREVK"
     gene            complement(30150..30857)
                     /locus_tag="PC471_001385"
     CDS             complement(30150..30857)
                     /locus_tag="PC471_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic protection ABC transporter
                     ATP-binding protein"
                     /protein_id="HDF2788520.1"
                     /translation="MSNYILQVKGVSKKFKEQMAVDNISLAIKSNSIYGLLGPNGAGK
                     STLLKMITGILNPTSGKIMFEGHRWTRKDLSNIGSLIESPAIYENLTARENLKVHTTL
                     LGLPDSRIEEVLETVDLRDTFKKRSGQFSLGMKQRLGIAIALLNHPKLLILDEPTNGL
                     DPIGIEELRELIRSFPKQGITVILSSHILSEVEQVVDEIGIISNGVMGYQGEVSKEQN
                     LEELFMKVVAENRKRGQ"
     gene            complement(30973..31509)
                     /locus_tag="PC471_001386"
     CDS             complement(30973..31509)
                     /locus_tag="PC471_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HDF2788521.1"
                     /translation="MNKIAVSFLIIATTLLSTACMDYSISAVELVDSKESAVVKKDED
                     AKEETTSKMINSKKTTKIPIEIISKDEKIVKYLQIDEESSLKDKLRLILDTLSNEYFN
                     GLDMEVEVKEKDNLVKINLIEPDKKSRVSWKDDYLNEQNIIYTINNIIKNVIQEEDNS
                     IWIEEVEIYYNGKLIELR"
     gene            complement(31611..32681)
                     /locus_tag="PC471_001387"
     CDS             complement(31611..32681)
                     /locus_tag="PC471_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat protein"
                     /protein_id="HDF2788522.1"
                     /translation="MNKKLAIFAAVLLVIGIIGTTWSGILVMPSLINFGLEKEAELNK
                     ENKLYQEKVNIDKLDIAVDNINVMIRKSSNEDVRVTTRGNNEFYKYSVTLQDKTLVVK
                     GERKSENKIKKIRSFDHLLNTSINSMFSRDYREIRIYVPNNVDINASSISSYLFISDD
                     VDSNNITYKTSSGGFSRAITENKVNRLENLNLISNSNLHLSTKSILGVKNVNIESESL
                     DISSENGDVFINNIEEYIPENVNIKEKIGRNSNYESEFYLYSDMPIAKFLDIEVPNSK
                     VRLDIPVNKYKFNCDIKSKEAIEEFNNDEEYDNDSYDYEHNEEHSNKYRNTREIKGLL
                     NKNLSNLEKEYTIKINSNSLEL"
     gene            complement(32674..33732)
                     /locus_tag="PC471_001388"
     CDS             complement(32674..33732)
                     /locus_tag="PC471_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HDF2788523.1"
                     /translation="MNLNKTEFLEILKDYLKKDFSESEVNDILRDYEEYFVDGLIEGK
                     SDMEIIASLGSPKSIAKELILQTKEKNIEEEKIINTDVLNDKFSKFKSDMKKGYSILK
                     DEAKEKYSKSKENIDGKIMNTDILNGKFSRFKSNVKKDYSILKDEAKEKYNKSKENIN
                     DKLTPSIEEGVGGLSRQVIKGLLVVLSLFLIVPAFAFVCAIVSATIALVCSLIAFVIL
                     IPFVIKFIAMVPDVMMFCIFATIAFVGLEILAWQILLFAIKLGKQLLNNYIHWIKRKN
                     IYIKANEKMEKRKKATLNDEDLNKDINENSYNNDYYNREEESIKETDEYNDDSINFEA
                     IYKDLEDIENKKGEDKHE"
     gene            complement(33719..34051)
                     /locus_tag="PC471_001389"
     CDS             complement(33719..34051)
                     /locus_tag="PC471_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HDF2788524.1"
                     /translation="MNTQFRKGVLEICVLALISKKDMYGYEIVHNISKVIDVNEGTIY
                     PILRRLTKDLYFETYILESNEGPARKYYRITSLGKENLSNLMEEWREFVKAVEILISE
                     DEGGNELE"
     gene            34250..35371
                     /locus_tag="PC471_001390"
     CDS             34250..35371
                     /locus_tag="PC471_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactosyldiacylglycerol synthase"
                     /protein_id="HDF2788525.1"
                     /translation="MKDDKTILILTAQFGAGHISAAKAVKECIIEKYSNYNVVIQNFI
                     NASIPMMNKPMVKLYENNTKYTPGLYNYYYYFKKSFDSRHDLSHKLYTPKLSEYIADI
                     NPDLIISTFPLAAACVNNFKIKNPDINIPTLTVITDVVDSMEWVFENTDLYFVPSPEI
                     KNRFFQKGINPDSIKVTGVPVDKRFQIESKELCCDKYRLLLLGGGRGLFDIDEDFMHW
                     IDEFIEEHSNSIEITIVTGKNKKLYDNLTHKKPLKNIKVLGFVNDMYNLIRECDLMLT
                     KPGGATIFEAIQSQTPVLVKMPKVGQEIENAKFIIDKGLGMIYSDDLDLKNIFYKLVS
                     NEFDSIINFMKKNLKEFKTVIHPEKIADYISELIDNHYS"
     gene            complement(35499..37397)
                     /locus_tag="PC471_001391"
     CDS             complement(35499..37397)
                     /locus_tag="PC471_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase"
                     /protein_id="HDF2788526.1"
                     /translation="MDSLLYVIEKDKHTNEELREILKSNKNIRFVSLMGVDLGGNATD
                     EKIPVELFLEDIDKFLESAIQTDGSSVELYNIATLNNAKVDLMPDKSCHWYVDYNMEY
                     IDEEVGLPVGTLKIPAFLIHDNKKVCSRGVLQKADKYFKKSMYEIFREYPHVINNIGI
                     DSVDDIEEIMLTAATELEFWVNTPEDKADLEKLYVSQSLKEQYWKRTHGIIRTCLEKS
                     LIILQKLGVNPEMAHKEVGGIQSSISIDGRTNHAMEQLEVSWKFSTPLQAADNELLVR
                     DVIEDVFTSHGLEVTFKAKPIHGVAGSGGHTHVGVSAKLKDGSIKNLFAPKDLKEDYL
                     SELGYGALMGLLYNYEVLNPIVTASNDGFNRLVPGFEAPVCIVTSLGHSYEIPSRNRS
                     VLVGLIRDMKNPKTVRFELRSPSPLSNTYLVIAGCYQTMLDGIKAAAKSGLSTKELEK
                     ELSKNVGEESFYLEKDRAYRDENDVFEHYSLEERNARFGIPPATVYENMKNLEIYASK
                     LKSLKQGDVFTDSIIESFKIGAIDKWQKKLKTRIIEAGIQKIRSIVKIHTKENMDALD
                     EVVWNSISDLKFNIMKDTLTRESLFTRVREAIENKDYQTASDLQIELKRSMEEIQQLY
                     MQYKKNIY"
BASE COUNT        12229 a         6309 c         4049 g        15062 t
ORIGIN      
        1 ttatgagcat attaatttaa gcaaaatgtc atttagactt taatttagta gttttatatt
       61 ataaatttat atatttttaa ttttaaatgt catttagaca ttactcataa aaagaaagga
      121 gtgtattttg tggatacttt aggaaaaaga attgcttatt tgagaaactc caaaaaactt
      181 actcagcgca aacttatgga tattttaaaa tttgaaaact taggcaagta tgaaacagga
      241 gatagaaaac ctaattgtga tatactaatg tcaatagctg attattttaa tgttacgact
      301 gactggcttc tctatggtaa agagaaggta aatgtcaata gtagtgtaaa agaagataaa
      361 gaagattact tacatgttac taatgatgaa atgacgatat taaatctata cagacaacta
      421 aacgaacgtg ataaaataaa aatagaagga atacttgaac ttaagatatc tgaatataaa
      481 gacttaaaaa agcattcctc taataataat gaagataaaa tggtttaaac atacataatt
      541 tgatacgatt ttacatcatc tattaacctt ctttttttgt agtatattac taaaatatgg
      601 atattggatt atttaaaaac aagtatagtc taatttcaag cacatttata atatttagat
      661 tatcttatcc atattttagt ttaagtactt tgaaaaatat aatacatctc tatgtataac
      721 ttatattcct aagaaatgca ttactcatgt ttttaattct tccaacatct ttctatacac
      781 aattgtacat ccttcataat tcccctcttt aagaaggatt gaacatgtac tagaatattt
      841 agttaaagta tttaatcttt ccatatttaa gctttttata ttactgagtg ctgtaataaa
      901 atcatcaatt gctctacaac actctataac cccatctgaa tccaatttac acacaagaat
      961 attcacctac tttatatact atttattaat catcttctga atattctata ttaagtaatt
     1021 gataaattga tgatttatat tttttacctg ttcttcttcc atttaaaata gctgacaaat
     1081 agttttcatt aacacctatt ttcttcgcta attccctttg agtcatattt aattcaataa
     1141 gtctttgctt gacttgcact cccaaaaaag gtatccattt ttttcgagca tccatttatc
     1201 aacttccttt ctaagtgatt tatatttact tttatattat tttagcttac acaccccctc
     1261 tatttagtat atatttttaa tatgtacaca tattaagtat ataaatttat ttaatactaa
     1321 ataagatttt ttgattactt tgtgctaaaa ttaggatgta aattcgtatc attaagttaa
     1381 ttataaagtc attttgacct taagtcaatg ctttttatgt cattttgacc ttaaatttga
     1441 aactatttac atcttaaaaa gtctttttga cattaattga atggagagtg tttatggata
     1501 ctttaggaga aagaattgtt tatttgagaa aagcaaaaaa ccttaaacaa tatgaactag
     1561 aagaaatgtt gggctgtgac aacttaagta aatttgagag aaacattaga aaaccaaatt
     1621 atgaaatatt aaaatctata gctgagatat ttaatgtttc agtagactgg ctattaaatg
     1681 gagataatct atcacataaa tcagatttaa tttgcgattc ctcctcaaac tatccactta
     1741 atagcataaa ctctaatgaa ataaagcttt taaacaattt tagaaagtta aatgattatg
     1801 ataaagccaa aatagaaggt atgattgaat taaaacttca tgaatatgaa aaagagaagg
     1861 atttaggaaa aattgagtat aacaaaaata aagatgagaa aatagataaa taatttttat
     1921 gttgcttttg atacgatttt acatcatttc tttgttctaa tatttttatt tcatttattt
     1981 tcttttttat aattttatga taaaataatt agtatatttt gctttacttg aatgtaatta
     2041 tttatacaaa agtcaaacat aatataaatg tagtaaattt ttagtttcaa gtaatatctc
     2101 gttattataa ctaaatattt attaaaatta ggaggataaa atgggcttaa gagacaaatt
     2161 cgcgcaatca tttgctagat caaaaactat gagtggccct gagaaaaagg ctaatgaaat
     2221 aatgggtaaa ttgcttctta agaaagctat attacctatc gttttaatgt tcgtgataat
     2281 aatcgctgga gctatgctta aaatcaattc ttgggttaca cttggcataa atttagttat
     2341 tgctgttggt gctttcttct acataagaaa ttctagtaaa aagtatcaga actttaaacc
     2401 ttatgttggc aatttgatta gtttagagaa aaaaggtaaa aaagagtacg ttgctattat
     2461 aaaacaaggt aagttaccag ttaagttaca aatagcttat ggtggtgaag accttgaaca
     2521 tgttaagaaa aatcaaatgg ttcaaataag ttataatcca gatgctaaaa tagctatttt
     2581 ggttaataga caataattta ataaaaaaaa ggagttgtct taaaattgat tttttctaat
     2641 caaatttaag ctcaactcct tttttaattt ttatatatta tttttatata ttatttttat
     2701 tacttttttt aattaatatt tttaaatatc aaatccttct caataaaata gggtactatc
     2761 tacaatacta ctttatcttt tcctaacaat atataatctg gtgctactct ttcatgttat
     2821 acaccgtttt gtaacatacc ttttatacca gtaacatctt ccttaatttc ctctacatct
     2881 gttttcatta tacccatttg aacaagtata tttttgttta tttcttgttg ttgtgtagat
     2941 aattctataa aattttctac agttttttta tacatgtctc tatcttcttt tttctcctgc
     3001 atagtatttt taaataatag agcacatagt atacctattg cccctagact actcaattcg
     3061 gttattaatt tatccattat agcctcctat tgtctaaatt ttttatactt atagctttaa
     3121 tttttgttat attcttacat catgcaatat tatattttca ggattatcaa tttaatcttc
     3181 tgtactttaa attgtaggtg caatttatag attgaataaa taataaatat atatacaatt
     3241 tctaatataa caaaaaataa gtacaaggct tacttattaa agttaagacc ttgtacttta
     3301 cataaaaaaa ataactaata catagttatt ctaaatttac tattcaactg gtatttctaa
     3361 ttctttgcat ctaattctaa cttgctctct aattactgct ggtacttctt ctattgtttt
     3421 tttgcctctt aaaattaaag ctacatatac ttctaccatt ccactctcac ctccagattt
     3481 tcttacacta aaagagctta tattacttgc ttcattgaca gaactttgat ttaacaacat
     3541 ctcatatatt tctgcatttg ctataaaatt atttgttata tcatcatctt gctttacttg
     3601 acctatactt atgtctgaaa tgtctttata ctgcatttct acttttctac cttgtttgat
     3661 ttgttctatt ttatttttat cgaatttaaa caattttcac acctccattt taagcttgtg
     3721 gctcgttgct ttcagcatca tatctaaaaa tcatagatgg acgttttata gctgtccatt
     3781 tatctccttt ttctactatg attttaggct gagcactttt tatgttggct tcttctaatt
     3841 catagatttc ttggtaactt gagtctgatt ttttgatttc aactagtttc atgtctttga
     3901 ataagtcaga tttatttatt tttacttgaa ctttagtttt atttattaat tgatatatag
     3961 gattgttttc catcatgtca tcctttaatc cctcatttaa tgttactatt aaatcagtgt
     4021 tattttcagt aatgtttatg attgaatata ctttattgtt tatcattaaa atagaatcct
     4081 tttttacttt aagccctgtt tctgatttaa atataaacat acttgaatat aaatcgcctg
     4141 taggactact ggctttaatc agtatcttat tttctcctaa tactatttta ttttgtggta
     4201 tctcaaatat aatgctattt atttccaaat tagtcttagt agatattaat tcatcattta
     4261 taaacatttc aagtttttct ataacattat tagggtcttg tactacaact ctcacttgag
     4321 atattgattt ttcaactatt tctatagatg ctttacttgt atctactttt gttatcatgt
     4381 atattgtatc atctgaaagc tctatatcta aaatatcatt tagatgttca ggaattggta
     4441 ttttagtaaa ttcatataaa tcagtaattg atatagatat tttatacata gatttatttt
     4501 taccagttat ccatagacta ttatcatttt taattactag tatgtggtca tcattatgtg
     4561 acttaatttc tttaacatta gttaatactt tagtaaaagt tcttattatc ttatcttttt
     4621 cactttctcc tagtccaaat aatccatttg tattgtaccc ttgagcgtac aactctccct
     4681 cattagttaa aacaagtgtc attgagttat atatacttat atctttaaca gaaggtaatc
     4741 cagtaacctt ttgtaactct cttacatcat tattgtggcc aactcccaat tggtactcag
     4801 tattctttcc atatgaccat agagtattat ctgtcttaat aatataggag tagttatctc
     4861 cacaagcaac ttttttgaca tctgatatat ttacttcagt aagagttaat aattctatat
     4921 cttcaagggt ttgattaatt tctttatatg aattacttcc tattccatat aacttgccat
     4981 ccttagcaac agccaatgaa tgtgtttttg atatatcaat atcagatacc tcaaatggca
     5041 atattattga agtatactta aaattatctt taaatatgtt tttataattt atatacttat
     5101 tatttgtttt tatatactta tcattaaaca ttactagctt ttcatttttc ttattaaaaa
     5161 ctattttatc tgatgtgtta tctgttttaa aatatgaatg tagggaacct tcaagagctt
     5221 catttttatc attatcatat ctatggtgaa tacctaaatg attatatcta ttatctcctg
     5281 ttgaagaata aaattttcca tatatatctt taataaatat agtattgtaa ctagaaaata
     5341 cagtatcaac atctttcttt atagatactt tataatatgg aactgacata tccctataag
     5401 attcatcaga tataccataa gtatctctac ctgatgatat caaattgtta ttattatcta
     5461 tataaagtgt attattagct gttacaaaaa catctttaac atcttccaca ctatttgtat
     5521 tattattatt tgctatactt ccatatccac taagacctcc aacaaatagt ttattgtcat
     5581 ttgttaaaat ggctgaagta tatccattaa ctactatttt ttttgcattt ataatattat
     5641 taattactcg tatacccttt atatcattag tttctgttga aaagaatacc ctctcttttt
     5701 tccctgtagt ataaataaca ttgtcatttg ttataaaaaa tgtagcttca cttcctgctg
     5761 ttacaacaga ttttacattc tcaatattta tttttgtaaa tgtacttata cttccttcag
     5821 taaacccaaa atatccattt acaaatccac atacataaca tgtattatca tttttaacaa
     5881 ttattgatac atcattacat atatacacat ctctaactcc atttataggt atacgtgtaa
     5941 atatattcct acttgtagta tcacccaatc ctaactgtcc ttttccatta ttacctgctc
     6001 catataaaag accatcatta gttaataata gagatgtgtt tccatataca gctacatatt
     6061 ttacattttc aacatttatt ttagtaaata catttctatt tacattatct cctattccta
     6121 actgaccaga attatttaaa ccaacagcat aacaggtatt atcatttttt atcgcaaata
     6181 catgtttact attgtcagta aatctattta catttataga ttttatatta tcaatattta
     6241 ttttagtaaa tacctttcta tttatctcat ctcccactcc taattgacct acatcattta
     6301 aaccaacact gtaaagcata ttattattag ttaataaata tgtagacttc gaatggctat
     6361 atatttcttg tatattagaa gatatttcac tactgctatt ctttttattg taatttatct
     6421 tattatttaa atttaaacta ctattaactg atataccatg cgtaccctta gagttatatc
     6481 cacatataaa taagctctta tcagttaata ttgcaaatct agttttatct ctacttaaat
     6541 cataaggagg tataaatata ttttttatat ttttcattac ctttttataa ttagtggcat
     6601 tatatccatc ttgtgtatct gaaacatcta gttcttttgc actttcagat atattcccaa
     6661 aaccatataa cgtgttagaa gataaataca atagagcttt atataaaaat atggctttat
     6721 cagaggtaat aggacctgaa tttatattag atgctgcaat attattagat gattgattgt
     6781 taagataatt ccttccatat tgatgtatat aagatgaacc tccagaaata agtaaattat
     6841 ctggatttac attttttaaa ttacttgttg attctccata atacaaatag gcaaaatttg
     6901 tagaatatga tgattgtgta caccttgtgg gataatagtt atatatgtaa ttactaatat
     6961 atccagcatg tgtaattcct tgttgttgaa ctctataagt agaatcactt ggctttgtcc
     7021 ataaagtacc atggtatcca gttaaaaaag aaccataatt tgtacataat atagccatat
     7081 attcatatat aataatatta ttaattttag taatatcatt tgtacaagaa tttgtactat
     7141 aacttgctcc tgaaggattt acataaaacc agttatttat taatgttaaa tttatattga
     7201 ctccatcatt aggattaaca cccaataaat agtttgtatt tataccagtt gcatatacct
     7261 ttttttcgtc agccacaata aatgttgcat tatatgaact atatgcatct gttacattgc
     7321 taacaccaat ctcagtatat tgacttgctg atttattact aagtccatta acataacttg
     7381 aactctttcc agaagtatat atttgcttat tattagttac aattaatgaa aaccagtttg
     7441 ataaatcagt atttataaca acttttttta cattatctat agatattttc ttaaatatat
     7501 ctgaattaga aggaagaaca cctgctattg agttactatt tggcccagaa taataacaag
     7561 tgtcatcatt tttaatgtag aatattcctc cagaaatatt ttttgatgct actatagact
     7621 ttacattaga gatacttaat tttgtcaact catttaattg acttggtaag tcagaaccca
     7681 ttcctttgta tctattattt cctagaccat atacatctcc attccctaaa ataaccagag
     7741 tacaataagg actaaaaacg acatctctta cagaatgtat atctagtgga agttcaatat
     7801 cctttatttc catttcttct tttaataaag ttaatttatc ctcagattta attaaattta
     7861 tataattact ataactatat ttttcactat ccattttctt aaattctaca atgtgcatat
     7921 catcaaaaaa cgaacctaat tgtccataag tattatctcc tgtacaatat gaatcattat
     7981 ttgaatcaat tagtatgcta tgtgataaac caatgaaaat attcttagct acaatatttt
     8041 ttaaatctat aaatgaatta tctatattct cagagtaatt tgctatacct aactgatata
     8101 tatcattatt tccagcaacc ttcaaaatac ctttattatc tataagcata gtattaccat
     8161 taccacatgc aatagttttt atatttactt cttctatctt agtaaatagt gatgattgaa
     8221 gtgcatttcc aaaacatttt tcctttccac aaccatatat atcattatta tacgtcatta
     8281 acacagtgtg ttcagaacca caagctatag tcttaacatc attaatagaa actaagctaa
     8341 ataaatctct attattctta tctcctaaac ctagttgacc ttgattatta aaaccagtag
     8401 aataacattt attgtcattc tttattataa aagctgattt attatatgag aatacagcct
     8461 ttacattatc aacatctact tttataaatg tatttacatt tgcagatgct ccattagttc
     8521 ctagacctaa ttgaccatac aaattatttc cagcaacata taaagttcca tcattgtata
     8581 aaagatatgt tgattgaagt ccacatgcaa tatcctttac gccttttatt tctggaacct
     8641 tctcaaatgt ggaaactgtt tttcctactt tttctaatcc caatcccatt tgaccatctg
     8701 tatttatacc tgcgacaagt aattctccat ctgaagtaag tataactgca tgagattctc
     8761 cacatgcaac ttttttaata ttagtagata tatttggtat tttcgtaaat aattttcttg
     8821 ttgtattgtc ttctcccaat ccaagttgaa agttactatt atctccacat ccatacaaga
     8881 tattattatt ttttagacca aatgtatagt tacaacatcc agtaaaaaaa ttaataaagt
     8941 cataattatc atacccttca tcaaattcat caactaccat atttttatcg ccaaagaaat
     9001 tacctctttg aagtagttta gttcttttca ttataaaaac ctcctaatta tcttatttca
     9061 tacgaatatt tatttaaagt tatatctgtc tggccaattg aatatgccct attagatttt
     9121 gtttcaacaa agttattatc catttcatgt atatatctaa ctctatttgc attaaatggt
     9181 agaaccacat tcttaaatgt aacactacca agttctccag ttatacactt tctatttaaa
     9241 tctaacttaa ggccttctat agctattatg tttttagcat tagttaaggt atcataccaa
     9301 tatccagctt catctacatc tatttctgac tctaattcta agtatattaa aatatctaat
     9361 actttttcag ataatttatt gtaactattt tttaatacag atagttcagt ttgtatttga
     9421 cctcctatta catcatcaat agatatattt ttaatttttt ctactacctc atttatagct
     9481 cctgataagt ttttagcttc tgtagataag agttcttctc caatttttac ttttatttct
     9541 tttatatttt tatcaacctt atcatctaaa tcttgaatat ccttttgagt tacaaaaacc
     9601 aacgtcgggt ctacttctaa tgtaacattt gaagtattgg acaattgtaa aatcattttt
     9661 acaaccaaat ctttaatact gccatcttca gcacgtggct tatacgtctc tggatactta
     9721 cctatagcta ttatattatt ctcattatcc agaacagcag cttctcttat cataaatcca
     9781 ccaactgttc ctggtaaaat agtctctata actatccaat taggattttt ttcatcaatt
     9841 cttagagaat ttatatttcc ttcccaaact acattcttta aagcagtttg ttcctctgtt
     9901 ggctcataat attctccatt actatctcca acttgaaact tggttaatcc aacaagctct
     9961 cctagtgcag ttgcatttgc tattttttct ctaccaattt tggttaatat tatataaaat
    10021 ttttcttcca attataccac ctccgattta ggataaacta ctacattttc tacgcctgta
    10081 tcatttgcaa tagctatatt aatcttacaa ttattttcaa tgcatttaat gcttaaagga
    10141 taaaccttta tttcttctct acttaacatt ccagctccac agtttacaac agcgctacat
    10201 tttataattg gttcaaagta tggatgaact tttacttctt cactacatac catacctcca
    10261 ccacaataga ccatactttt atctagtatt attggttcta aatagtgtaa taagtgagca
    10321 ggttttactc tttcaatcat atcgctacaa tctaaaagag ttttatagtc acaattattt
    10381 gctataaaac tcaatacgaa tgtaaattca tcactataaa cttttatatc tgtttctgat
    10441 tttgtatatg cctcacatat actttttata acttcaatag tactagtacc tctgcttctc
    10501 attttagctt ttatattgct acgtcttgtg tcaaaatcaa gttctttttt ttcaatgcag
    10561 agtatgtctt cccacatatc taatccccat gttgcagtgt caacaaataa ttggttaaag
    10621 gtatcatcaa tatcttcttt aagaatattt aattctgtat cgtatgcacc ttgtatctcc
    10681 tctacaatgt aatttctatc aaatgatggt agtttatcaa ttagcttcat tgattttcca
    10741 cctcactaaa attaacagtt gttacactag gtattttatc ttcattaata gttatattat
    10801 ctgtacttcc atttataagt aggttactta aatcgtgtac accagtagta tttataagta
    10861 ttcccattac ttttgtatag attatctctc ctctaatatc tctaaagtat gtatttatac
    10921 tttctaggaa agattctttt acattgtcta atgtatatcc atcttctaat ttcattactg
    10981 cacttatatt tatttctatt ggtaatggtg tcacaactgt tatagttggt ccaataggct
    11041 tctcttcatc tatatgttgc tgacaccttt caattgtttc tgtatcaaca gcttgattat
    11101 ttttcccaaa tatcaagacc ttaactgttc ccggaccatc ccatcttgga taaaccttaa
    11161 cattatagac tccatctact tccaaagccc attcttcata gtgagcttta tttccacttg
    11221 tagcttgatt tctttggatt ttataaaatc tttcttttag ttcttcatct gtctctatat
    11281 ctgtaccacc ttcaaaactt cttgtgttag taatctttgt tactccagat atattatcaa
    11341 ctagtttaaa ttcacaattt gtaggtaaat tatatttctt accaacttcc agagcttgaa
    11401 ctgggctatt gtcaccttct tcactaccaa tagttacatc ttttattact acaaatagta
    11461 aatctctata tgatattatt gtgccattat ttattacagt tcctttctct ccaataaatt
    11521 caacttctcc atttgactct gtacctaact ttctatatac accaaattca ttgactcttt
    11581 tatcgagaaa ctggttatat gtgtcttgta taaaagccat tttatgtatc tttgaaagtt
    11641 ctagatatat ctttgaaagt tctagattat ttccagatac catgttgttt agaaaagaac
    11701 cttctccttt ataaatatca agattaatat tttcaagagt tctatttttt attacttcat
    11761 atgtctggtc actatacatt tatttccacc tccccataga tagtggatac ttttacattt
    11821 gcactcaata cgtctctgtt aaagttagca ctttttacgt ttacttctaa tatatatgga
    11881 tttattagaa gggcctcttt tatgaatcta ctagcttcac tttctgtaag accttttgta
    11941 tatttttgtc ctattagttc tgaaagctct gtcccatatt ctaaagagta tatctcatgt
    12001 tcatatctat ttgtctttat acacttgtat atccaaactt ttaaggcttc ttttttttca
    12061 attattttaa agtcaccttt ttctaaaata ggttcatctt tttcaaaatc ccatgccact
    12121 tcacgaaaga ttggcaattc ttctgtttta ggtaagatat aatcctctgg gacacctata
    12181 aaaggaaata ttgtactcat tataagctca ccaacttact tacaacagca aatttttcgc
    12241 ctattctaaa cattattacc atatccccag attcaaaatt atctataaaa ggatttttta
    12301 cttcatgctg gtgttcttga tttgtttctg tgtcaaatgt ttctagctgt ctatcaatca
    12361 tccaactatc tatcaaaata tcttcttttt ctaatatgat gttatttgtc tctattttta
    12421 aatctggtaa tttactttta atttttccaa taaaaaaaga aggttgatta tgatactttc
    12481 cttcttctct tattattcct ataaattcat ttattggatt agccattata tcaccatcct
    12541 tttataatac tctcctagct gtattaaaat ctcttctact acttaactta cttattttaa
    12601 ctacatcacc tttttgtgga gcatgtaaaa attctccatt acctatatat aatccaacat
    12661 ggctgactgg attatgaaaa aatactaaat ctcctgcttg aagatttttt tgttctactt
    12721 tcttgccttt tttagattgt tgatttgacg ttcttggcaa actaacatta acttttttaa
    12781 aacaatagac cattaaacca gaacagtcaa aactacttgg tccatttcca ccccatttat
    12841 aaggtttacc tagatgtttt tttgcttctg atattactat tttagcttta tcagatacat
    12901 taccagaata accaccaata ataatttttc cttttcttct tccaaattta ctagcttctt
    12961 caacatttcc aaataaaata tctatatggt atgttccatt cttttctata gttattgcag
    13021 gtcctacgtc atttacttta tagactccat cacgaccaga aactcctgta attacctgta
    13081 tttcatctcc atagtttaat aaaggatgtt tatttaaaaa ctcttttgta taataagttt
    13141 gttcatattt accaaccata ggagcagcgc aagttttttt agatgggtca agttttttct
    13201 ttctacaatc tgtatctcca ccttcttctt ttctaggaca gtaagctgta aattctgctg
    13261 taaactcttt tcctcctgca taatcttctc cccctaagtt actttcttcc ttaggttcat
    13321 cctgtcctgc tgacttttca tccataagat tttgaaaatt aagttcaagc tcaatttgat
    13381 attctccatt ttgccaagta tgtttgtctg tatctatata aaatagtcct acaagctttg
    13441 tataagaatc tttaactttt actcctctac cagttataca acttacatct ccataacctt
    13501 taagagaaca gcttttttct atcccattaa actcgctatc aatatctaca tcttgatttt
    13561 cttgttgctg aattactttt tgcattatta catttacttc cttaaaaatt tcattatcta
    13621 ttttttcgct aatcttgctt ccatactggt ctaccactat tactttattt tttacatttt
    13681 ccatgctctc cgaaaaggtg gtatttataa tattaaatcc ctcttcaaac ataacattta
    13741 atgtaacagt tcctttttca ataacattaa acttatctaa attggcctct atcatatact
    13801 ttttctttgt ctttttactt gcttctgtat atgcactcat tattgtgtca taaccattta
    13861 ctcctataaa catctttgta tacttgacat tggtctttgc tattgttcca actgaaagcc
    13921 tattttcagc aaatacttgc tttgctatgt cttcaactaa cttatcttta aaattgtatg
    13981 acacttcact ttgtgtaagt aaaaatccca tatctttaga tacaaaacta atttcattac
    14041 tgcttgaatc tttagaccta ttaattatca ttcctctaaa gagttctttc tcatctacat
    14101 agaaacagac tgtactagct attggtatat ctatttgttg gaaatttaca tcaaatgatg
    14161 attgtattat tgaaaactct agtgtccttg atggagattt ataatcacct gaccatgata
    14221 ccttgtctac tatgtcagtt atgtcatata tacttccgtt ttttatgtgt acccaaatct
    14281 taataataaa aaatcacctg ccttttcatt taattctata agcagttatt tataacttat
    14341 tatctataat agttattgtt ttggagttac ttttggtatc tttattcttt tatactcttt
    14401 taaatctaat gtaaaatata catctcgagt cccatctctt tcttcataac taaacccttc
    14461 aattatgact tccatattta tatttgtttc cgtaattgta aatcttaata taaaaccttc
    14521 cttcatccat ttttctatct tatttacaca atcatatggt gatggaaaac ctgtatagtc
    14581 acagaaagta tagtctttat ttggaaaaaa acttgatatt gaaatgcttt taagagctac
    14641 accaccaaaa gttgctattt cacctatttt gagtatacta gaagagttta cagcagcttt
    14701 tccattaata ctaaaggaag atggaaatac tggaaatcta aatctatctt cagcttgtct
    14761 aagccacatt tccattataa gacaccccct aactgtgcca attggatact tgcaactaat
    14821 tttgatgtta tcttttctat atcagcttct tctctaatca ctattgtatc agctaactta
    14881 gctatgttta tatttccacc attttttctg cttctatatt gatttgcttc ttgtttagtt
    14941 aaaaccattt caccttcatg gagtctggtt tgatagtcat tataaggaac atagttcaat
    15001 ccaaatgcat tattaccttt cttacctgaa taagctttac cagaatcacc agcctttgtt
    15061 ccagcatatg gattatcttt gtaatttttt ttactctttt ccactagatt taaaactgtt
    15121 cctgtgatgc ttccattttt tatcttatta atcatagttt gccaccactt gcatatatct
    15181 ttaactattt tacatacttt atctaatatg ttaaaaaaag ctcccaatat tggctctata
    15241 attttccatc cagtttccaa cagaggtcct aaggtctccc atacagtttt ccaaataaca
    15301 cccagtgtct ctataaaatt gtttatttga ggtgcatatt cacctaaaaa agtaaaaatt
    15361 cttgtgataa taggactaac agcttcaact acaggtccga ttagttggaa tgctgtagtt
    15421 actattgtac caaatatact tattaatcct tgtgcaattg gtgatttatt gtttatacta
    15481 tctagtaaat tatttactaa gtttactact ggaagtaatg aatctattaa tgctgtaaaa
    15541 tcgatatttt gtaatagtgt acttcctgcg ttaacaattg cttttggtgc attccctatc
    15601 attgttgctg catgattttc aaaacgttct tgcttatttt caaccatcat tttacgcttt
    15661 tcagcctcat tagaaattcg atattgagta gaatcagaaa actcttttcc attttttaca
    15721 cttctagatt gcatctggtc atatttgttg ccaataatta tcttaccatt cattacattg
    15781 cctattttac cagccatttt ttcagataag ccagctaaat tgcctaatcc actttctata
    15841 aaagttttta ttttctttag tccttgacct aaaatatcat caaaaggttt aacaatactc
    15901 ttcaagccat tgttaatgct tgtttgagct cctgatatta tacctggtat tgtattactc
    15961 atttctttag ctaaccccat ctgctttaca cttatgcctt acaatgcttc atcaaaagtt
    16021 ttataatttt ctcctaccag agtattaaga gattccatgt ttccttttgc tgcacttaag
    16081 aaagctgctg atacatcttg ttcactactt gtattcatat ttaaagctct aacatctacc
    16141 atagcttgag ttatatcttt ttcttttttg ccaccagtaa tattactaat cattttagct
    16201 ttagttccca ttgcaacaac atcttcaggg tcaaaaggcg tatcatttgc cattcttact
    16261 aaactgccat aataatcatc aacatctatt tgattaactt ttccagtatt tcttttcatt
    16321 acactttcta gaacaacttt ctgattttgt tcctgaccaa gtaaatcaac accctttttc
    16381 acagcgacac ctatagtagc accatttgtc aaatcagata tcttcccttt tatcgaacca
    16441 agtattccac ttgccatatc ctttacagct attgcaccac tccatacctt acctgcaaat
    16501 aatctcaatt tgggaaatac tttacttata atgccacttg ccatatcttt tatagctatt
    16561 acaccactcc aaaaagtatt tttaaacaac attaacttag gcaaaattct acccatagca
    16621 ctactcacca tatctttaag attaatactt ccttgataag gacgtttctc tacttgtttt
    16681 aattttccca agatactact tataactctc ttagtcatat cttttgcttt tattacgata
    16741 ttccaaacct tgccaccaat cttttttaaa tttccaagta atttattttt agttttttct
    16801 acttcatcac ttttagcttt aaatattaat ataatcataa tatttgaatt aaactcattt
    16861 ttcttttgaa tcaacttatt aaaataatca ataatcatag ctatagttat tgctagtatc
    16921 ataatacttc catttaaact tccaataact cctgaaacag catttatgct agaacttaat
    16981 tttgtcatag ttgaacttac actactagca gtagtttgaa cttttcttaa cgaggaattt
    17041 aaactgttta ctctattact tgcacttcca atagaatttt ttatgctact agccattaca
    17101 ttagtggaaa gtgaaacagc cacagttgct ctcctaaaat tatcagttac tgatataatt
    17161 tgattcattt ttggtgtgta attatctgta aggtacatat ttataccttc ttctctcaca
    17221 tttcccatta cataccacct cctgtcaaag gacacactcc atatccatgt ttcatttttt
    17281 caacttcttc ctcaatttct aaggcgaaaa aagcttcaat aagctttaat tcgcctttat
    17341 ccattgcata aaaaagggac ggtcttatac cttttttctt ccaataatag tacattattg
    17401 tagtcctggt atccgtccct attagttttt tacctcttct atcactgcat ttttaccata
    17461 acccataaca tcttgaagta ttctatataa gttggtaatt tcacctggta ataataattt
    17521 tcttgctaat tcttttggtg ttggagcctt aaatttatcc attagctcct tatttttaaa
    17581 taaaagcttt ccatccaaat cacaaacacc ttctataatt gtaagcatct gcatctccgg
    17641 taagtctata tccgttttca tattttcatc aattttaaga caagtatttt gtatttcttc
    17701 atctttactt attgttaaag ctctacacat tactttaaat tcctgcccaa atatagttga
    17761 taatctctta agttcaacta tagtactcgg tctttctaag ttttctgtat ctgaatttaa
    17821 aagtaaatct actatgttta tattttttga taatccattt tcactcatta ttatttgctc
    17881 ctttatcaat caaattcaat caactaactt ttttacttat taactatctg aatttattaa
    17941 gtatatattt actaaatatc agtataaaac caaatataaa actaaatata aaactaaatt
    18001 atatcaagaa aatcatactc agtaaatgtg aaaggtgctt ctatctctcc ttttacgcca
    18061 acctcccaat cagctaaagt taaatcatca aatgctatat tctttactgc tattctttct
    18121 gctccataag aatctgggtc atttaattta gatatagcaa caaacctagg ttcagaacct
    18181 ctctttatct tttctccaat taacttgtgc attcttgaac taacgtggta tagagttatt
    18241 gagccttttc ctttatatcc tatatacttt gtatcagtac ccatttgacc tgctattata
    18301 atatcttctt ttgtaaattc catctttgct tgaaatttct ttacttctgc tactttgttt
    18361 ccatcaagcc aaagttctcc ccaagtacca ctcattacat ttctagcttc catatttgcc
    18421 atattaataa tcctcctata tttctattga taaatctata tcttccatag catcaagtac
    18481 ttttattttt gcttttaaaa atactttaga acctgtgtta gcttctttta tttcttgtaa
    18541 tgtcatataa gataaatcta ctccttttga ttttaaatac gatttttgtg cttcaaaatc
    18601 tatttcaaca gtagaatcag attctataag tgctgatttt tctaattctt ctaaataact
    18661 ttttatagct actatcaata aacatttgtt gtcataactg ttagtaacct ttcctatata
    18721 gtcatctatt atcacctttc ttatgtcact atgtataata tctaaagtgt caactatttt
    18781 tattttctgg aacatttctc ctttttctgc tgttaattca gttaaagaat ttactcctct
    18841 agcaattctt atagctcctg cttctttaat aagtataagc tctcctttat taacctttga
    18901 ttctgcatca actttcgtca tcttaggtat atcgactaca tcactaagtt tagtataagt
    18961 tactgattga cttaaaggtg tacctgctat aagtccagcc accctacttg taaactcatc
    19021 aacactgtat ttcttttctc caactaacac atcttctgta gtaaaattaa ttatcccttc
    19081 atgatttcct acaacttttc ctaatacagc tttaacctta accttatcta tatctctaag
    19141 tttaattatc caatttttta tagcagtctt atcagcttct actgcttttg gcatacatag
    19201 ataattaaat tccttagtct ctaaaaaatc taatgcagtt tgaatatctg cttctccttc
    19261 tattacataa actaataact tatttggagt gttaacattt cccatcaaag ctaaatttat
    19321 atattcttta ttttcagcag ataaatcaac tggtatatcc tctttttcat gtatttcatt
    19381 aagacctagt gccttagcat ctttaagtac cattgcaatt attcctctag ctgaacgttc
    19441 tttaacagtt gtagctagct ccttaaatga tatgttgata cttggtaatc ctatagccat
    19501 actatcactc tcctaaattt aaattgattt cttccatatt ttcatatact acatcttctt
    19561 catatacact atccagatag tttagtgtaa atgtaaagtt caatacataa attgactctt
    19621 cttttttaat actttgttct agctttgtta tatttaattt tctgtctgtt acctttatat
    19681 ttctgttgaa taacttattt aattcatctg aaacttcata cattctaata ctctttgact
    19741 gtggttttgg taagtatttt atagagataa caatatcttt gttattcgta tttaacatta
    19801 caggtgtaca aataactgat agtatactta caaaaaaaca agacccttca gagccttgca
    19861 cactttcatc atcaataaaa atactttcag taaagttttt tttgagaatc tcaataattg
    19921 tttctaatat ttccttgtat ttaagcaaat aatcaccaca ctatcactaa atattcttgc
    19981 ttaatctgat aatcttacta taaatgatat aagtcatttt taaatatata tctcatttat
    20041 tcctgtcaag tatcttctat atttttaggt aatattattc ttttcaatct atcacctcct
    20101 tcaatatgga tttctttcac aataacatta tctcacatat tttattgcct attgttgtaa
    20161 tggtgtatat aaagtgttgt aaaattgtcc tattttttat aatatctcac tcaatttaaa
    20221 tatcatcttt ttctttaaat tgtagcaaga tgttctatct aagtgtagtt tcatagaaat
    20281 atatgtcata ttgttttttg tcttactatt ataaaaaaga ttaaaaaatt ccatttctat
    20341 atcatttaga cacgttagag cattttctat cttctctttt tctatttcta tatcttcttt
    20401 ttccattttt aattttctta atctttcctc tctttttaac acctcatttt ccacagaaga
    20461 gcttatgtta tatgtgttac tagtcttttc tgtatatacc attgctccac atccagagta
    20521 ttcattctca catcttttta tgtccaagtt aatattcttt atctttatat ccaaatactt
    20581 ataattatgt aatcttttat ctgtttcttt aaataatata tcttttttgt tttccatagt
    20641 acttcccctt ttttatatac ttattattag cttcaaattt acaataatct ctttagtgca
    20701 cagtttttta ctaatctcac aaccttcaag aacatattgt tctataactg tataaaaaaa
    20761 acttcttcta tattttgttc taaaatacta gaaatcttct tagccataag aagtccagga
    20821 ttttttatgc ctctttctat ttgagatata taatcatttg taattccagc ctttctggca
    20881 agttgaattt gtgtcatacc tttgcatttt ctataatatt tcaagttatt tttaaacatt
    20941 gttttatacc tccattacta ttaagaatat tttgttcttc ctcacttaga ttatatagaa
    21001 ttatttgttc tttatcaata gattttagaa aaaaatattc ttttatattt tattagtttt
    21061 attttttaga acaatttgtt ataatctata cataaggtgg tgttataatg tttgccaaaa
    21121 gattaagaga acttagaaag gaatttggat tgactcaaag agaacttgga gaaaaagtag
    21181 gcgtttccca aagagtctta gggtactatg aaacagaaaa cagatttcct gatgaacaca
    21241 ttttaaataa attggctgat gtatttaatg tatcagttga ttaccttctt ggaagaacct
    21301 tagttaagga aaatattgat acagtagctg cacatagaaa aaatccacat gaagagttac
    21361 ctgaagaagc acaagaacaa cttaatgatt atatagaatt tttactaaat aaatataaaa
    21421 aaaaataaac catatgatga gcagtctatt tttctgctct tttaatataa ttcaaaggca
    21481 aacatacatt ctattttagg gggattttta tgaataattt ggataagctt tttgaattag
    21541 cttctcaaga agagataata attcattata ccacttatat tgcaggtgat ttagaaggac
    21601 tatatataaa caaacatggt attaaaatta tatcattact cagtaattta aaacaaaact
    21661 caaaaaaatt gacatccatc ttagctgaag aacttggaca tcatttcact agccttggat
    21721 actatgtatc ctcttataac gattattata caaaaattat tatagataag tgcgaaaata
    21781 aagccttaaa atgggcttgt gagttcttaa ttactgaaga agatataata aacataatta
    21841 actctggcat cacttgcgtt tatgaaatgg cagacatact taatgtggat attactttct
    21901 ttcaaaaaag attagaattt ctatcattaa aaaaacagtc tttacaactt ggaaataata
    21961 aatatttaat attaactaat ttgccgtatt tctacatatt tgatccaatt tcataataaa
    22021 aatagtagta attttaactg gcttaacagt aaaatctata tatttatttt aacaaatttt
    22081 ggtttctata aattcaatat taatatataa taatgatatc atattattta aaattctaaa
    22141 aacaaatata tgcttttgtt aagaaaggtt gtttgatgaa tgtataaaga aaagttgttt
    22201 gataattatt ttaagttctt agcactatta ttttggccaa ttatgtggta taagtggata
    22261 gtaatttcaa atggtactct tgaaaatatg ttatttacca tatatgcaat cgtcgcaatt
    22321 gttttcatca ttttatattc ggtgtttatg ataaaatata aagacattac acaaatagat
    22381 ttcttttaca ggataagtac tctacttgct tttatattta cactttttag ctttttaata
    22441 tacccaaaga gtttattttt cttgtattta aagattattt ttactggaat atacctttat
    22501 tattcaatag taaaaactct taaatttaaa gatgacgaag gtgtagtggg tattatgagc
    22561 tcacttttat taattgtaat aactttgttc tactaaattt aattctattt gacttaatat
    22621 ggctttaaat attagacatc tacattattt tctgatatta tttaagatgg tagtcttata
    22681 ttaataatga aatacactat gaaggagtta actattatgg aaaaaaaagt tttagcaaca
    22741 gttggagaaa aagagataac taatattgat attgaaaatg cattaaaatc attagaccct
    22801 tatcaagcta tgcaatttaa aactgaagaa ggaaaaaaac acttattaaa tgatttagtg
    22861 aatcaagaat tattcttctt agatgctaaa gaagaaaaat tagatgaaga agaaatattt
    22921 aaattagaaa tgaaaaagat agaagaaaat gtattaaagc aatttgctat aaataaagtt
    22981 ttatctagtg ttaatgtaac agaagatgaa aaagtaaaat tctttgaagc taataagagc
    23041 tctttctcta aaccagaaag tgcaactgct aaacatatat tagtagatag tgatgaaaaa
    23101 gctaaagaaa tacttgctca aataaaatca gaagaaatat cttttgaaga tgcagctctt
    23161 aaacattctt cttgcccatc taaagatatg ggtggtgatt taggaacatt cggtagagga
    23221 caaatggttc ctgaatttga agaagctgtg ttctctatgg ctaaaggtga agtaagtgaa
    23281 ccagttaaaa ctcaatttgg ataccatata ataaaattag aagaccttca agaaagtaca
    23341 gagtctacat ttgatgaagt taaggctgaa gttgaaaaaa gtttattata tcaaaaacaa
    23401 aatgaagttt atggaaataa gataaatgct cttaatgcta agtacggaaa ccttgtaaaa
    23461 tacaacgatt aatcataagg tcatatataa aaaagctgtc ttttcacaat atttattaga
    23521 aaagacagct ttttatatta aagtttaaat attcagtttc atattatcat ttttttattg
    23581 taaagaactc agcttaaaag tttaaataag atttttaatt atcattagct aaatttttat
    23641 atccacgtat tatctcataa cagtaaccta ttacatctct tctttttatt atcccaataa
    23701 atgtgtcttg gtcatctata acaggaatga aattttgatt taaagatttt ataactaaat
    23761 cttcaatatc tgcgttgatg gaaacaggag aattatccat ttttctattt atatctgtca
    23821 ctggaacatc ttccaatccc tttaagtcca gatttaaatc attttttaat gtccataaaa
    23881 aatcaccttc tgttatagtt ccaacatatt taccatcctt acttattatt ggaattgcag
    23941 agtatttgtg atattccatt ttttctaggg cttgccttat agtgtagtct tcatatatat
    24001 atgctacttc actttttggt gttagaaaga ataatatgtt cattacacta cccaccgtta
    24061 ctgaaaactt ataaatatta ctcaatattt ataatgttac ttttttgcag taactttctc
    24121 ctttcttatt ttctaagtat tcttaatact atacgctgta acaagcgttt ttcctttgct
    24181 cattttaatc ttattatgct ttattaatat ttttatatgg tctataagta tcacttattc
    24241 tctttttaga aaatttacta aaaattaatt gacttaaact tagccataat aattttactc
    24301 tacactatta attttaacat atttatctca ttattaatga ttttatttta ataattttaa
    24361 tttattttgc tattaatttt atttacaaaa actgaaaaaa ccgctaaatg attagcggct
    24421 tatctctcat attactagaa cactaatata tatgaatttt taattataat ctaactacgt
    24481 tagcagcttg aggaccttta gctccatcta ctacttcgaa ctcaacgctt tgtccttctt
    24541 ctaatgtttt gtatccatct ccagttatag ctgagaaatg tacgaaaaca tctccttcac
    24601 cttctacaga tatgaatcca aatccttttt cgttgttaaa ccatttaact acaccagttt
    24661 tcattaataa atcctcctaa aaaaacacaa atatttagct actcttttct gtaaccttat
    24721 tactactcta tcatatatag ctatgtttgt caattattat tatttttata cattaataat
    24781 caattttttt actttataat tttaaatgat tattatcttt tacactttat ttttctttat
    24841 aattttttaa taatttattt ttattatatg gaatcttatg gacttatcac tccagcctaa
    24901 atcccatggc accaacattc tatcagtatt atggcaagtt accaaaggat atcctcttga
    24961 gtctaatcct gtcactgtcg aaacatgtgt tattctgcct ttcttctcat aaccaacaaa
    25021 atcccctgga cgtaaattat aagcttcttt gtatacttct tcataggaac ctttagcaat
    25081 taatgaacct cttccactat acagtgcata atttttaaaa gcttgtgcat ttacccatgc
    25141 ttttgtacct gctctctcgc tataattcca aattgagttc tttttgaacc ttccactttc
    25201 aaacattatc tgtgatgcaa agtttgcaca atctccacct tctggattat aatccctata
    25261 gtttggatta aatttcatac catgttcttc atcagctgcg gctccacagt atttatgtgc
    25321 ataatctata gctttttttt gttcatctgt aagctgtaac tctacaccat cttgttgacc
    25381 aatataattt cttatatcat cagattttat attttctaaa tttaaagagt ctgcaaaagg
    25441 gtctgtatac cactctttag ttatgacata ttgattatct ataattttta catttagata
    25501 atgatatgta cctattctaa aggcattttc tttgtccttt tgattttcat atgaatactt
    25561 atattcagtg gatactgaac agattatacc atagaggtct ttttcttttt cctttatttt
    25621 tttaatctta acccttgtct ttatgtcttt aaattttaca ccttgtttac tagaccagtt
    25681 ttctaaatat ttcatctttt ttacttcatt ttcataagcc cacagaccaa attttttatc
    25741 cgtttcataa agacctttta aaatattttc atttttctct aatattgcct tatttctgta
    25801 gtcaaataag ttttcaagca atagtttata ttggtctttt acatcatcac cttcaacatt
    25861 tgcagattcc atgacttttt cttccatatt tttcatactt aaatcattga ttaaaaatac
    25921 acctgctgta gtagctaaag acaaaaatac aaatgccatt atacctttga tgtacttatt
    25981 cttatttttc agtattttca aagctacacc tcccaaatag tacaccttac aaatcaaatt
    26041 acaaagaata ttctgtcaca tatagtaatt ttcatattta ataattctct attttattat
    26101 gtccaatatt aaaaattaat tgtatcaata cactgttttt tttacaattt tttattttat
    26161 aatctacttt tttctttttt ttatagtaat atatatgtat ataaatattt tggtatcatt
    26221 ataattaata catactaatg gagggttttt tatgcaaaaa aaagtcgcag caattaatga
    26281 cttatctgga ataggaaaat gctcattatc ggttgctata cctatacttt cagcattaaa
    26341 ggttcaatgt tgtccatttc caacggccat actttcaagt caaactggtt atcctgaatt
    26401 tactttctta gactttactg atgaaatggt aaaatattca aatgtttgga aaaatttaaa
    26461 agtcaatttt gatagtatat atagtggttt tttgggttca aagcatcaaa ttgagatagt
    26521 ggctaatttt ataaacgatt atcccaatgc ttttatcgta gttgaccctg tgatgggtga
    26581 taatggagtt atgtatccaa tattcacaga agaaatgaga caggaaatta aagaattagt
    26641 taaacattca gatttaacta ctcctaattt aacagaagct tgtttcttaa ctggaaatga
    26701 ttataccaaa agcgattata atagagatga gcttatatat attgcaaaat ctgtatctga
    26761 tttgggacct agcaaagtcg ttattactgg tatattagaa gatgataata tattaaattt
    26821 ggcttacaat agagataatg accatgtatt ctttacatca gtcaaatata ataattgttc
    26881 ttacagtgga actggagata tattcacatc tatactttgt ggtatgttag taaataaaca
    26941 tgatttggga gtagcggtta atactgcaac agattttata tataaaacta taaattacac
    27001 atctcaattt gatactgata gaaatgatgg agttatgttt gaaaactttt tatcagattt
    27061 aaccaatata taaaatcaaa aaaactatcc attttataaa tacctataaa tcataaaatt
    27121 taggtattta caaaatggat attatttact aaactattgg tatttcaatt gttaccatag
    27181 caccaccagt tttcacagaa ttttcaatac ataaagtccc attatgtttt tgtacaatat
    27241 tatttacaat atagagtccc attccataat gagatttaga atttctactc aaatctcctg
    27301 tataaaactg ttctgtagct tttgacaaat ctgctttaga aaaaccactt ccacaatctg
    27361 taactacaaa ttttaagtat ttattatgta ctgatacact tatgtatagt ttactctggc
    27421 ttggtgaata atctatagca tttgaaatta tattcataat agctctgaat aacagttctc
    27481 tatctatagt tatattttta ggcaaattat cttgtttata aaactccaca tctaagtttt
    27541 tagtactagc taatgcttct atttgcacta aaacatcttt tataaactca ctcatactga
    27601 ttgcttcttt atttaatata tagccattct ctgtttttga tatatctatt aaagtattaa
    27661 tatatttttc tatttgagtg acaccctttg agatatattc catgtattct aataactcag
    27721 gattttcatt tatttcaatt aataagtcag tatttccatg tataattgtt agtggtgttt
    27781 ttatatcatg tgccagtgca gatatctgtt cctttctatt ttcttctagc atccattgtt
    27841 tttctaaaga atcttttaac gccaacttca ttttatccat agaaaaaagg acattattaa
    27901 tttcataaat tttactagat tctacaacaa aatctaaatc ctgttgttct attttttctg
    27961 tagtattttt taaaagctcc atttcaatat taaatttctt gccaaatact tttgataata
    28021 taacaatttc aataaaaaat attatactaa aaataatcat acccaatgtc tctggttttg
    28081 gtaaatatgc tcttaaaatt ggagaactat actctgatac tattttgtat tttattataa
    28141 aaacttcatc tttcttaaaa aactttatat aactttctga atatgcatgc ctttcttcaa
    28201 tatctctcat taaattccat acttcttttg attctttttt attaaaggtc cctgaaatta
    28261 aattcccatc caaagtataa accccatact catatatact tggaattata tcttcagtta
    28321 ctttttcact tgaaataatc tgttctttat atttattaaa ttgtttttct gcataattag
    28381 ctggtaaaat tacacctgat gataataata cagaaaatga taacattaaa aggagtacaa
    28441 ttgaaattgt cataataaag aataaagata aatattttat aaaaatagtt cttaactttt
    28501 caactttttt gttgttagat tttttacctc caagcatttg taccctgtcc tccaatagtt
    28561 taattaattc ataattaata cccaaccagt tttttatttg attattttat atctttttat
    28621 tgctaagtta tattttaaca taataatata aataaacagt caatttttac tctattcttc
    28681 tgatttttta ccttcccatt tagaaaacca aatacataaa attacaaatg ctaaaattgt
    28741 tacaaaacca caaattaaac ctgttgattg atatgctttt attgattcaa cctttatata
    28801 ttcaaccgtt ttagaacttg aaaaagacga ccaaatagtt ataaatctaa caccccatgc
    28861 acaaggaaaa tactgccaaa taccagaacc caatccagtt aataataatg ctgaaagtaa
    28921 catttcaact atacctactc caattgatgt tccttttccg aaccttaaac ttaagaataa
    28981 atgtaatatg tattcaaata aattacaccc aattaaaatt agaattgaaa tcatataaaa
    29041 gcttgatgta aagtaagcct tagatatacc aaatttaaaa cctaatacag acactaatgt
    29101 tgctacaaaa ccaagacata ctaagattat atatttactt ataagagtaa gatattttgg
    29161 attagatgtt gttaacatat gtttataatt ccctgcataa tattcttgtt caacaaacat
    29221 tgaacacaca acacttgaca ataaaggaaa agctattgct ataacttgta aatatgctac
    29281 tatctttagt atattatcag ctcctgaaac cttataatac cataaaaaca atcccatccc
    29341 cattaaagca atgcaaatgt gcattagtat tataaaattt ctctttaatt ttattaaatc
    29401 agaacaaaat attcttatta actgtctcat attacttcac ctctctatta ttgaaccaaa
    29461 cccttgtagc taaagcaaat aatacaaata aaacaataga tattaatact cctggcaaaa
    29521 taacagcatt atttaaaaat tcacttccct ctggtacagg aagaccattt ggcagaatat
    29581 gcaatacaac agccattaat cttgaaggaa tcgaatatgg aaacatatac cacatttctg
    29641 tagttgagaa aacagcaact ccaacaatag taaagaacat atttgttaga actgtaacaa
    29701 acataccaaa ttttgaagct aaaaacatac aaagaggaat ctgccacata aatagtaaaa
    29761 taattaatat acttgcaatg atattttgac ctattgaaat agtttcttta tagattatcc
    29821 caacaaaaac tacacctatt gcagaaataa ttgatgctat aaaacaacaa ataatacagt
    29881 aaatcatttt tccagtccaa acgtctttca tttcaattgg caatggtaaa ataccacgat
    29941 atttactttt ttcatctttt aatactatta aagaagatat taatgttaat gctcctggaa
    30001 gaaatgtcgt ataccaccag ttgaaagcag cattttgccc tccacctagt ccacacaaaa
    30061 gtatacaaaa cactggaact atccatatca acttcattcc aaaagttcct ttcatctttt
    30121 gaaattccac tctaataaat ctttccatat tactgccctc tctttctatt ttcagctaca
    30181 actttcataa aaagttcttc taagttttgt tcttttgata cttctccttg ataacccatt
    30241 actccattag aaattattcc tatttcatct acaacttgtt caacttcaga caagatatgg
    30301 cttgataaaa taactgtaat tccttgtttt ggaaaagaac gaatcaattc tctaagttct
    30361 tcaataccaa tagggtcaag accatttgta ggctcatcta agattaaaag ttttgggtga
    30421 tttaataatg caatagcaat accaaggcgt tgcttcatgc ctagagagaa ttgacctgaa
    30481 cgctttttaa aggtatctct taaatctaca gtctctaaaa cttcttcaat gcgagagtca
    30541 ggaagaccaa gtaaagtagt atggactttt aaattttctc gtgctgttaa attctcatat
    30601 atagcaggag actcaataag agaaccaata tttgataaat cctttctagt ccatctatgt
    30661 ccctcaaaca ttatttttcc tgaagttgga tttaaaatcc cagttatcat ttttaataaa
    30721 gtagatttcc cagctccatt tggtcctaat aatccatata tagaattaga tttaattgct
    30781 agtgaaatgt tatcaactgc catctgctcc ttaaattttt tagaaactcc ttttacctgc
    30841 aaaatataat tactcataat tttcatcctt tcttttgtca aacttattta caagaatcat
    30901 tctaaatcat aaatataagg attttataag aattaaaaaa caaaaaaaga tgtgttaaaa
    30961 acacatcttt ttttatctta attctattaa ttttccattg tagtatatct ctacttcttc
    31021 aatccaaatg ctattatctt cttcttggat gacattttta ataatattat ttatagtata
    31081 tattatattt tgttcattca aataatcatc tttccaagaa actctacttt ttttatctgg
    31141 ttctatcaag tttattttaa caagattatc tttttcctta acttctactt ccatgtctaa
    31201 accattaaaa tactcatttg ataatgtatc taagattaat cgcagtttat ccttaagtga
    31261 actttcttca tcaatctgta agtactttac tattttctca tcctttgaaa ttatctctat
    31321 aggtatttta gtagtttttt tagaattaat cattttacta gttgtttctt cttttgcatc
    31381 ttcatccttc tttacaactg ctgattcttt agaatcaact agctcaactg cactgatgct
    31441 ataatccata catgccgtac tcaataaagt agtagcaatt attaaaaaac ttactgctat
    31501 tttattcata agtatccctc tcctaattct ctttcgatac ttattatacc atagtataga
    31561 ttatattttt atacttttaa atcaatttat aaattattat agtaaattat ttacaattct
    31621 aagctatttg aatttatttt tatagtatat tctttctcta aattcgacag atttttattc
    31681 aaaagccctt ttatttccct cgtatttcta tacttgttgc tatgttcttc attatgttca
    31741 taatcatatg aatcattgtc atattcttca tcattattaa attcttcaat tgcttctttt
    31801 gactttatat cacaattaaa tttatactta tttactggta tatcaagtct taccttagaa
    31861 tttggaactt ctatgtccag gaacttagca attggcatat cagagtacaa gtaaaattca
    31921 ctttcataat ttgaatttct acctattttc tcttttatat ttacattttc aggtatatac
    31981 tcttctatat tattgataaa tacatctcca ttctctgaag atatatccaa tgactctgat
    32041 tctatgttta catttttaac gcctaaaatt gactttgttg atagatgaag attgctatta
    32101 gatattaagt ttaagttttc taatctattt actttatttt ctgttatagc tctgctaaaa
    32161 cctccagaag aagttttata tgttatatta tttgaatcta catcatcaga tataaataag
    32221 tacgaactta tacttgatgc atttatatct acattatttg gtacatatat tctaatttct
    32281 ctataatcac gtgaaaacat actatttata gatgtattta ataagtggtc gaaacttcta
    32341 atctttttaa ttttattttc agattttctt tcgcctttta caactaaagt cttatcttgt
    32401 agggttacac tgtatttgta aaattcatta ttccctcttg ttgtcacacg cacatcttca
    32461 tttgaacttt ttcttatcat aacatttata ttgtcaacag ctatatctaa cttgtcaata
    32521 tttacttttt cttgataaag tttgttttct ttgtttaatt cagcttcctt ttctaaacca
    32581 aagtttatta ggcttggcat aactaaaatc cctgaccatg ttgtccctat tatacctatt
    32641 accaataaaa ctgcagcaaa tatagcaagt tttttattca tgtttatctt ctcctttctt
    32701 attttctata tcttcaaggt ccttatatat cgcctcaaaa tttatactat catcattata
    32761 ttcatcagtt tcttttatgc tttcttcttc tctattgtag taatcattat tatatgaatt
    32821 ttcattaatg tctttattca aatcttcatc atttaaagta gctttttttc ttttttccat
    32881 cttttcattt gcctttatat aaatattttt tctctttatc caatgtatat aattatttag
    32941 taattgttta cctagtttta tggcaaataa taaaatttgc catgcaagta tctcaagccc
    33001 tacaaatgct atggttgcga aaatacaaaa catcataaca tctggaacca ttgctataaa
    33061 tttaatcaca aatggaatta atataacaaa agcaattaat gagcatacta atgcaatagt
    33121 agcagataca atagcacata caaacgcaaa tgctggaaca attagaaata aagataacac
    33181 tactaataac ccctttataa cttgtctgga taatcctcct acaccttctt ctatacttgg
    33241 tgtaagttta tcatttatat tttcttttga tttattgtac ttttccttag cttcatcttt
    33301 caagatagaa taatcctttt ttacatttga tttaaatcta ctaaatttac catttaatat
    33361 gtctgtattc attatttttc catctatatt ttcttttgat ttactatact tttcttttgc
    33421 ttcatctttt aagatagagt aacctttttt catatctgat ttaaatttac taaacttatc
    33481 attcaaaaca tctgtattta ttattttttc ctcttcaata tttttttctt tagtttgtaa
    33541 gataagttct tttgctatag attttggtga gccaagtgat gctattatct ccatatcact
    33601 ctttccctct ataagaccat ctacgaaata ctcttcataa tctctcaata tatcattaac
    33661 ttcgctctca gaaaaatctt tttttagata atcctttaaa atttcaagaa actcagtttt
    33721 attcaagttc attacctccc tcatcttcac ttattaaaat ctcaacagcc tttacaaatt
    33781 ctctccactc ttccatcaga ttggataaat tctcttttcc tagtgaagtt attctatagt
    33841 attttctagc aggaccttca ttagattcta gtatatatgt ttcgaaatac aaatcttttg
    33901 taagtcttct tagaatagga tatattgttc cttcatttac atctattact ttagagatgt
    33961 tatgtactat ctcatatcca tacatatctt tcttagatat gagagcaagt acacaaattt
    34021 ctaaaacacc ctttctaaat tgtgtgttca tatactccat cctttcttta aatacacagt
    34081 attgtgtttt acaaggtact atgttaaaac ttcttatatc ctcataataa cacagtacta
    34141 tgttctgcac aatagttatt tttaaatatt cttgttgatt aaatccttct ataaatgtat
    34201 aatactctaa tcagatatat tttatgaaag tggtgataaa ttggctaaaa tgaaagatga
    34261 taaaacaata ttaatcctaa ctgctcaatt tggtgctgga catataagtg ctgcaaaagc
    34321 agtcaaagaa tgtattattg aaaaatatag taactataat gttgttatac aaaactttat
    34381 aaatgcaagt attcccatga tgaataagcc tatggtaaaa ctttatgaaa acaatacaaa
    34441 atatactcct ggattatata attactatta ttactttaag aaatcgtttg attcaagaca
    34501 tgatttatct cataaattat atacacctaa actttctgag tatattgctg atataaatcc
    34561 agatttgatt atctctacat ttccactagc tgctgcttgt gtaaataact ttaagataaa
    34621 aaatcctgat ataaatatac ctacacttac agttataaca gatgttgtag atagtatgga
    34681 atgggttttt gaaaatacag atttgtattt tgttccttct cctgaaataa aaaatagatt
    34741 ttttcagaaa ggaataaatc cagactccat aaaggttact ggagttcctg ttgataagag
    34801 atttcaaatt gaaagtaaag aactatgttg tgataaatat agactattac ttctaggtgg
    34861 aggaagaggt ctatttgata tagatgagga ttttatgcat tggatagatg aatttattga
    34921 agaacatagt aattctatag aaattacaat agttactggt aaaaacaaaa aattatatga
    34981 caatctaact cataaaaaac ctctaaaaaa tattaaagta cttggatttg taaacgacat
    35041 gtataatcta ataagagaat gtgacctaat gctaactaag cctggaggag caactatatt
    35101 tgaagctatt caatcacaaa ctccagtact cgtaaaaatg ccaaaagttg gacaagaaat
    35161 tgaaaacgct aaatttataa tagataaggg tcttggcatg atttatagtg atgacctaga
    35221 ccttaaaaac atattttata aattagtatc aaatgaattt gattctataa taaactttat
    35281 gaaaaaaaat ttaaaagaat ttaaaacagt cattcatcca gagaaaattg ctgattatat
    35341 atcagaatta atagataatc attacagtta aattgtacta ttaaaaaatt tcaaacataa
    35401 aattaaaggc aatcaagtat gcttttacac ttgagaattt actcatagtg tttaataata
    35461 cttgattgcc tttatcatat caaaatcaaa ttgttatatt agtatatatt tttcttgtac
    35521 tgcatataca gttgttgtat ttcttccata cttctcttta attctatttg taaatctgaa
    35581 gcagtctggt aatccttatt ttctatagct tctctcactc tagtgaatag tgattctcta
    35641 gttaaagtat ccttcataat attgaacttt aaatccgata ttgagttcca aactacctca
    35701 tcaagcgcat ccatattttc ttttgtgtgt atcttgacaa tacttcttat tttttgaatc
    35761 ccagcctcta ttattctagt ttttaatttc ttttgccact tatctatagc tccaatctta
    35821 aatgattcta ttatactatc agtaaataca tcaccttgtt ttaaagattt taattttgaa
    35881 gcatatattt ctaagttttt catattttca taaactgtag ccggtggtat tccaaatctt
    35941 gcatttcttt cttccaaact ataatgttca aatacatcat tttcatctct atatgctcta
    36001 tctttttcta aatagaaact ttcttcacct acatttttag ataactcttt ttcaagttct
    36061 tttgtactaa gaccactctt agctgctgct tttataccat ccaacattgt ttgataacat
    36121 cctgctatta ctaaatacgt gttagataga gggcttggtg accttaactc aaatcttaca
    36181 gtctttggat ttttcatatc tcttattaat ccaacaagca ctgacctatt tcttgatgga
    36241 atctcataac tatgacctaa agatgtaact atgcatacag gagcttcaaa tccaggaact
    36301 aatctattaa atccatcatt agaagctgtt acaattgggt ttaatacttc ataattgtaa
    36361 agaagtccca taagagcccc atatcctagt tcacttagat aatcttcttt taaatctttt
    36421 ggcgcaaata gattttttat acttccatcc tttaacttcg cacttactcc tacatgtgta
    36481 tgtccaccac ttcctgctac tccatgaatt ggttttgctt taaatgtaac ttctagtcca
    36541 tgtgatgtaa atacatcctc tataacatct cttactaata attcattgtc tgctgcttgt
    36601 aacggtgtag aaaacttcca agaaacctct aattgctcca tagcatggtt tgttcttcca
    36661 tctatactaa tagaactttg tattcctccg acctctttgt gagccatttc agggtttaca
    36721 cctagctttt gaagtataat taaacttttc tctaaacaag ttcttataat accatgagtt
    36781 cttttccagt attgctcttt taggctttgt gatacatata atttctctaa gtcagcttta
    36841 tcctcaggag tattaaccca gaactcaagt tctgtagctg ctgttaacat tatttcctct
    36901 atatcatcta ctgaatctat tcctatgtta tttattacat gtggatattc tctaaatatt
    36961 tcatacattg actttttaaa gtacttatct gctttttgaa gtactcctct agaacatact
    37021 tttttattat catgtattaa aaatgcaggt atctttaatg ttccaacagg tagacctact
    37081 tcttcgtcta tatattccat attgtaatct acataccaat gacaactttt atctggcatt
    37141 aaatctactt tcgcattatt taatgtagct atattataaa gctcaacact tgaaccatca
    37201 gtctgtatag ctgattccaa aaatttatct atatcctcca aaaataattc tacaggaatc
    37261 ttttcatctg ttgcatttcc tcctaaatca acccccatta aggaaacaaa tcttatattt
    37321 ttattgcttt ttaggatttc tcttaattct tcattagtgt gtttatcttt ctctattacg
    37381 tataatagac tatccatttt tccactctcc cataattttt atgtatattt tattttgtta
    37441 aatacaagat aaatttcatt ttccttacat ttatgaattt ttcttgcaaa ttattcgtgt
    37501 tttattcgtg tttttattta tattattata tatcaagtat aaaaagttcg caatattttt
    37561 tattggaaaa aatattatac atacaatagt ttaacatttt tacaaatatg gggtatacta
    37621 aaataaaagt atttttattt gaaaattta
//
LOCUS       DAMJYR010000029        37568 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.029, whole genome shotgun sequence.
ACCESSION   DAMJYR010000029 DAMJYR010000000
VERSION     DAMJYR010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 37568)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37568)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37568
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.029"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            12..410
                     /gene="tnpA"
                     /locus_tag="PC471_001392"
     CDS             12..410
                     /gene="tnpA"
                     /locus_tag="PC471_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011291581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HDF2788527.1"
                     /translation="MYKSNKNIVYDCKYHIVFCPKYRKPVLVGEVEKMLKEILPYKAD
                     ELGAEIIEMETDKEHVHLLISCDPQYGIHKVVKGLKGFSSRVLRENFPHLKSSMPSMW
                     TNSYFVGTVGSVSLEVVKQYIENQQVRTKK"
     gene            407..1570
                     /locus_tag="PC471_001393"
     CDS             407..1570
                     /locus_tag="PC471_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDF2788528.1"
                     /translation="MIKTHRVKLNLTRTQFELVREKQMESANCWNYIVNLSKEYYFEH
                     KQWIKKNDIQKLIKGKYNLHSQTIQAISDKFDANRKTISELRKKGNTKAKYPYKTKKF
                     YIIPFKASAINRNAKGNLKLSMSKGRYLELDFNVENIKTAEIVWRNGYYLYYTFDNEL
                     NGVVAKGTNTVGVDLGEIHSIASVTNKGVGLILSNREGRSIKQFRNKMYAYISKRLSK
                     CKKGSRQSKKLWRLKNKIRSKTDNQLMNLYHQTTRKFIDFCVEQKVCEIVLGDIKGVE
                     KDTKKKKRLNRVNRQKISQMEYGRIKDYIKYKAKEQGIEVKLVKENYTSQTCPKCSKK
                     HKPTGRTYSCTCGYETHRDIVGAWNILNKKHKYGLVDFRINHKQPINLKVSTV"
     gene            complement(1954..2466)
                     /locus_tag="PC471_001394"
     CDS             complement(1954..2466)
                     /locus_tag="PC471_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HDF2788529.1"
                     /translation="MLEVIKNRRSIRTYIDKNIEEDKITEILKSAMQAPSSKNAQPWE
                     FIIVDDKELLKQLSKSQHRAKHIEFAPLCIVVLGNRDKFLKPGKWIQDLGACTQNLLL
                     EVTNQGLAACWAGVFPKNKVVNKVRQTLDLPLKLVPYALISIGYSEEKNEFIDRFDEN
                     KIHRNVYKNR"
     gene            2916..3392
                     /gene="purE"
                     /locus_tag="PC471_001395"
     CDS             2916..3392
                     /gene="purE"
                     /locus_tag="PC471_001395"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HDF2788530.1"
                     /translation="MKVAVVMGSKSDYPKLEEGIKLLEKYGIEVVARALSAHRTPEQL
                     SIFLKEIEDDTDVIIAAAGKAAHLPGVIASQTLIPVIGLPIKSSTMDGLDSLLSIVQM
                     PKGIPVATVTIDLGLNAALLALQIMTLKYPKLKEDLKSYREEMAQKVLEDDNNLRG"
     gene            3398..4096
                     /locus_tag="PC471_001396"
     CDS             3398..4096
                     /locus_tag="PC471_001396"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007285750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HDF2788531.1"
                     /translation="MLLYEGKAKQVYSTDNENEYVVYYKDDATAFNGEKKAEISSKGI
                     LNNKISTIIFEMLKENNINTHFIKSLSDREMLVKKVEILPLEVIVRNIAAGSICKRVG
                     LEEGVVFDEPIFEISYKNDAYGDPMLNDDYAVAMKLATREELKFLREETLKINELLKA
                     FFLKLNLKLVDFKIEFGKDSEGNIILADEVSPDTCRLWDVNTNEKLDKDRFRKDLGDL
                     VEGYTEVLSRMNNK"
     gene            4108..5475
                     /gene="purF"
                     /locus_tag="PC471_001397"
     CDS             4108..5475
                     /gene="purF"
                     /locus_tag="PC471_001397"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HDF2788532.1"
                     /translation="MCGVLGIYSNKDVTKELYYSLYSMQHRGQESCGLALLDDGEIKY
                     KKDMGLVGDVFKENELSKLKGNIGIGHVRYSTAGGSHVSNCQPLVGSCRKRQLAIAHN
                     GNLVNANYLKDMLEEDGYMFQTNSDTEVILYILARYYKGDIVESLKVTMDYIKGAYAL
                     VIMSQEELVAVRDPHGFRPLVLGKKGDEYIFASENCAIDILGGEVIRDVEPGEIIVVK
                     DGELKSYFYSENYKPVKKSCIFEHIYFARNDATIDNVNAYEFRIKCGERLAQNETVKA
                     DMVVPVPDSGWPGAIGYANASGLKISEGLVKNRYVGRTFIKPTQEEREIAVKIKLNPL
                     STIIKGKSIILVDDSIVRGTTSKQLVKSLREAGAKEIHLRITSPPVAYSCYYGIDTPN
                     RSKLIASSNNVEEMREYIGCDSLKFLDIEGMLDATEHKSTFCKACFDGEYPVKKIDKE
                     ELLSC"
     gene            5469..6545
                     /locus_tag="PC471_001398"
     CDS             5469..6545
                     /locus_tag="PC471_001398"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HDF2788533.1"
                     /translation="MLTYKESGVDIDEGNRAVDLIKGKIKGTYDGNVVGDLGNFSGLY
                     SLKDFVGMKEPVLLASTDGVGTKLKIAQMMDKHDTVGIDLVAMCVNDLICQGAKPLFF
                     LDYIALGKLVPEHIEKIVGGIADGCKMSGCALIGGETAEMPGMYGEDDYDLAGFSVGI
                     ADKEKIVSGNNVKSGDVLVGISSSGVHSNGFSFIRKIFLETYNYKMERYVEELGMTVG
                     EALLTPTKIYVKLALDVLAKHDIKAVAHITGGGLIENITRVIPKGLGLDINKKSWEKP
                     PIFKMIEGFNAVDERELHKSFNMGIGLVLIVDKENADDVVNFINNRENDNADYVDKKY
                     SELLEDKAYIIGEVVDSHEGVELC"
     gene            6539..7132
                     /locus_tag="PC471_001399"
     CDS             6539..7132
                     /locus_tag="PC471_001399"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HDF2788534.1"
                     /translation="MLNIGVLISGGGTNLQAVIDGTESGEIKGQVKVVISSKQGAYGL
                     ERAKNHNIKAICETDEDKIIEILKENKIDLVVLAGYLKIISPKLVNEFRNKMINIHPS
                     LIPSFCGAGFYGEKVHQGVIDYGAKVTGATVHFVDEGADTGPIIMQDVVKVNQDDDAK
                     TLAKRVLEVEHRILKESISLFCENKLKLQGRRVFINE"
     gene            7125..8657
                     /gene="purH"
                     /locus_tag="PC471_001400"
     CDS             7125..8657
                     /gene="purH"
                     /locus_tag="PC471_001400"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HDF2788535.1"
                     /translation="MSKRALISVTDKTGVVEFAKELNKLDYEIISTGNTFKTLKENGV
                     NVMQVEDVTNFPEILDGRVKTLNPYIHGGILYKRDKESHVETVNEHKIHSIDLVAVNL
                     YDFEGTLKAGKSHDEIIENIDIGGPSMIRSAAKNYKDVIVVVDIKDYDSIIEKLKTDT
                     MTLEDRKKLSYKAFSTTGRYDALISSYFAGEVGDTYPDILNLTFQKEQTLRYGENPHQ
                     NGFLYSQSNAKNPILNYEQLGGKELSFNNLNDLHGCLEVMREFKDSEEVVSVAIKHAN
                     SCGVGLGKDAFEAYTKCYEADKVSIFGGIVGITSTIDKATAEKLNEIFLEIVVAYDFE
                     PEALEILKQKKNLRILKLAKIENSLQPYEMKYLDGKLLIQDRNNILAEKSENVTKEKP
                     TDAQLKDMEFGMRVVKNMKSNAIAIVKNGQTLALGCGQTSRIWALKNALENNKDKDFT
                     GAVLASDAFFPFDDCVTLAHEYGISAVVQPGGSIKDKDSIEACDKYDMVMVFTGIRHF
                     KH"
     gene            8668..9918
                     /gene="purD"
                     /locus_tag="PC471_001401"
     CDS             8668..9918
                     /gene="purD"
                     /locus_tag="PC471_001401"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HDF2788536.1"
                     /translation="MKILVVGGGGREHAICWKLSKEKNVEKIYCAPGNAGIANVAECV
                     NIGDTNIEELLKFAKENEIGLTIVGPEVPLVMGIVDEFEKEGLRVFGPNKKCAQLEGS
                     KAFSKEFMIKHNIPTAKYKEYTNLEEAISEIDSFGYPVVIKADGLAAGKGVVIPENRE
                     DAIATLKEMMSDKKFGAAGDKIVIEEFLKGIETSILAFVDNDTIVPMASAKDHKKVNN
                     YEQGPNTGGMGTFSPSEIYTEELANKVKETVLEKTLEGFKKDGLNFKGILFVGLMITE
                     DGEKVLEYNVRFGDPETQSVLFRLETDLHEIMEAILDNKLKDIEINYSDDEAVCVMLT
                     SGGYPDSYEKGKIITGLENLDDDIVVFHSGTKMFDGNLVTNGGRVIGITAKSTTVKDA
                     AEKVYENIKKINFEGMHYRTDIGR"
     gene            9943..13749
                     /locus_tag="PC471_001402"
     CDS             9943..13749
                     /locus_tag="PC471_001402"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HDF2788537.1"
                     /translation="MLNTENKDSMVRRVLVEKREGFDLEAKALKKDLVESLHIDNIEN
                     LRILNRYDVEGISEEVYENAAKTIFSEPNLDVVYYEEIPKLNDERVFAIEFLPGQYDQ
                     RGDWAAQCVQIVNQGIRPAINTAKVYILSGKITDEEFSKIKDYCINPVDSREASLEKP
                     ETLKMETEIPTTVEVLDGFIDLDENGLKTFVSEKGLAMTLGDLQHVQKYFKDTEKRNP
                     TITEIKVLDTYWSDHCRHTTFMTEIENVKIEDGKFNDIVKEAYQMYLNSRDNVYVNRH
                     KDICLMDIATVAVKELKKNGKLNDLDESEEINACSINVDVEVDGKMEKYLVMFKNETH
                     NHPTEIEPFGGAATCLGGAIRDPLSGRSYVYQAMRVTGSADPRTTLEDTLPGKLMQRK
                     ITTEAAHGYSSYGNQIGLTTGQVAEVYDENFVAKRMEIGAVIAAAPKENVVRERPEAG
                     DVIVLLGGKTGRDGCGGATGSSKEHSEESILTCSAEVQKGDAPNERKIQRFFRNKEVA
                     QMIKRCNDFGAGGVCVAIGEIADSLDINLDLVPKKYDGLDGTELAISESQERMAVAIK
                     KENKDKFIQLAVEENLEATHVATVTDTGYLRMFWNGKAIVDINREFLDTNGVKQTTDV
                     HVTKVDEENTFFSSNEIVKDVKCASMKDKFTKVLSDLNVCSQKGLVEMFDNTIGGNTV
                     LMPFGGKYQATPTQGMVAKIPVLGGETNTSTIMTYGYNPKVGKWSPFHGALYAVVESV
                     CKLVAIGGNYSTTRLTFQEYFEKLGNNPEKWGKPFSALLGAFYAQSKFEIPAIGGKDS
                     MSGTFKDIEVPPTLVSFAVDTVDAKKVVSPEFKKADSKVVMLCVNKAENDVVDFEELK
                     RNLDKVRELIHGNKVLSTYALGFAGVGEAISKMAFGNKIGFKFSEEAEKAFTDDKLFE
                     ASYGNIVLELANDDLSMLEGYNYVVLGSTVKETSIFIKGEELALDELYKAHCSTLEPI
                     FPTKTEEVKSKIETISYISQGEAKKSSLSIATPRVFIPAFPGTNCEYDSARAFERAGA
                     NASIRVFKNLTYKDIEDSIDTIVNEIKSSQIIMLPGGFSAGDEPDGSGKFIATVFRNP
                     RVQEAINEFLTQKDGLMLGICNGFQVLIKLGLVPYGEIRVPSESAPTLTYNNIGRHQA
                     KIARTRISSNKSPWLAQTNVGDIHNIAISHGEGKFVASEDVMRELIANGQVATQYVDF
                     NNEATYDIEFNPNGSFYAVEGITSADGRVFGKMGHSERIGEEVYKNIIGEKDQKIFES
                     GVKYFR"
     gene            13955..14827
                     /gene="rfbD"
                     /locus_tag="PC471_001403"
     CDS             13955..14827
                     /gene="rfbD"
                     /locus_tag="PC471_001403"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HDF2788538.1"
                     /translation="MKILITGSNGQLGKELVNQLEAINQSINQPKYVILATTRSDLDI
                     SNQTNVDNFILHNKPDVVVNCAAYTKVDACEDNIEIAYKINALGVRNLAIASEKVNAK
                     LIHISTDYVFNGFSKYPYREDNKTEPNSVYGKSKLMGEKFVEQFSHKYFILRTAWLYG
                     NGNNFVKTMIKLSLENKEVNVVNDQFGTPTSTVDLAKIIIKIMETEHYGVYHATCEGE
                     CSWYDFAKKIFKLKNIDIKVNPIKSNEFKCKAPRPLYSVLDNFMLKLIGLNSFRKWEE
                     SIEEYLERYDINEK"
     gene            14936..15805
                     /gene="rfbA"
                     /locus_tag="PC471_001404"
     CDS             14936..15805
                     /gene="rfbA"
                     /locus_tag="PC471_001404"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HDF2788539.1"
                     /translation="MKGIILAGGSGTRLYPITKCISKQTLPIYDKPMIYYPMSVLMLA
                     GIQDILIISTPRDIKAFEELFGDGSSLGLNITYAIQEKANGIAEAFIIGEEFIGNDRV
                     SLVLGDNIFYGYGFSERLENAVKRKGATIFGYHVSNPNSFGVVEFDKDFNVLSIEEKP
                     TVPKSNYAIPGLYFYDNDVVEIAKLVEPSDRGELEITSINSEYLKRGKLKVELLGRGM
                     AWLDTGTHKGLLDASNYVEVVQTRQGLYIACLEEIAYTKGYISREQLMKLAKPLIKTE
                     YGQYLLKLASEDM"
     gene            15854..16411
                     /gene="rfbC"
                     /locus_tag="PC471_001405"
     CDS             15854..16411
                     /gene="rfbC"
                     /locus_tag="PC471_001405"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HDF2788540.1"
                     /translation="MGKINVIKTKIEGLYIVEPNVFYDERGYFMESYNKKDFIEQGLD
                     MCFIQDNESSSRKGVLRGMHFQVKYPQGKLVRVTEGEVFDVAVDLRKDSQTFGKWEGV
                     ILSDKNKKQFYIPEGFAHGFLVLSEKAVFSYKCTNYYYPEFENGFIWNDKQISIEWPL
                     KGIEEIFLSEKDKKQKSFKDICTRR"
     gene            16449..17432
                     /gene="rfbB"
                     /locus_tag="PC471_001406"
     CDS             16449..17432
                     /gene="rfbB"
                     /locus_tag="PC471_001406"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HDF2788541.1"
                     /translation="MKKILITGGAGFIGSNFIHYMLNKYVDYFIVNLDLLTYAGNLET
                     LIEIQDNSNYKFVKGDISNREQVYKLFEEERFDIVVNFAAESHVDRSVENPDLFIKTN
                     IIGTQVLLDASIKYGVKRYHQISTDEVYGDLPIDRKDLFFTEQSSINPSSPYSASKAS
                     ADLLVSSYYRTYGLLTTISRCSNNYGPYHFPEKLIPLMILNALENKQLPVYGNGENVR
                     DWLHVYDHCTAIDLIIHKGNIGEIYNIGGHNERSNLEVVKMILNLLGKSEELISYVND
                     RPGHDLRYAIDATKIENELGWKAKYDFDLGIKETVKWYIENESWWKAVLSV"
     gene            17462..18130
                     /locus_tag="PC471_001407"
     CDS             17462..18130
                     /locus_tag="PC471_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HDF2788542.1"
                     /translation="MNEISSLINILALTQLSGSNTTNQCNCTYNNSSGFDMIMMTLLK
                     ALSQNNQHTSNSYNLLSNSENLFNELDNVVNNTTSRFIDVDTKDKNVKLRIENAVNQA
                     SKKYNVDSNLIKAIIKVESDFNPNTVSSAGAKGLMQLMPENCRDLGVTDPFNIEQNID
                     AGTRHIKEYIDMFGGSIEMGLMAYNGGPGRMKSRGVESISDLYKMPKETQNYIPKVMK
                     YYRG"
     gene            18139..18567
                     /locus_tag="PC471_001408"
     CDS             18139..18567
                     /locus_tag="PC471_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaaR family protein"
                     /protein_id="HDF2788543.1"
                     /translation="MGINIDKLNISPSINMEVKKLKESNKFSDKLNSLKQSSSLEDIK
                     RNLESVKKAGERLVLTQNYGDISIYKNAVKEYLKSVVDNMYSLDKNSSFWEHQYYKNV
                     KIVDSKLEDMTSKLISEEKENIDIVSTVDMIQGLLIDMYR"
     gene            18591..18905
                     /locus_tag="PC471_001409"
     CDS             18591..18905
                     /locus_tag="PC471_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor switch protein"
                     /protein_id="HDF2788544.1"
                     /translation="MNSKKEDIYELNFGQLIEEKSGVIEENQNVFNAKVKVAVSIGNT
                     TETIKNILDLKEGSIIKLSKNVDEKLDIYANDRLFAYGESIITNEKLSVRLSKIEDSD
                     VN"
     gene            19056..19331
                     /gene="flgM"
                     /locus_tag="PC471_001410"
     CDS             19056..19331
                     /gene="flgM"
                     /locus_tag="PC471_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HDF2788545.1"
                     /translation="MDIKNVSSNIVVNNYLKNTSGIKKVEEVKKINKTDKIELSKFAK
                     EMASVSNMSDENIRNKKVDEIKNALENGTYKIKPKEIAKKMIEDMRG"
     gene            19336..19752
                     /locus_tag="PC471_001411"
     CDS             19336..19752
                     /locus_tag="PC471_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlgN"
                     /protein_id="HDF2788546.1"
                     /translation="MITDLKVIIYEEKNIISKMMELLKEQFDYIVEKDLENLNMLNPK
                     LEDISRELASIEIKRRQLFGNDISMSEVVENSNDGYLKEIYTDLKRILALAKNQQESN
                     DSLIKKELIFTKKMINFIKPADKQTTTYNSYGNIRK"
     gene            19770..21080
                     /gene="flgK"
                     /locus_tag="PC471_001412"
     CDS             19770..21080
                     /gene="flgK"
                     /locus_tag="PC471_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HDF2788547.1"
                     /translation="MAGLFSILNTANSGMNAHQKAIQTISHNISNLDTDGYSRQRTEF
                     ATNSPMYMPSLSNSIARGQLGTGVHVTDVTRARNSFYDYQFRAEAHKYGKTVSKYDYY
                     NTIETILNEPSDYGISAGIDDFFKGWESLSKDINSDSKKSLVVENASNLATLISESYK
                     KLDKLKEDIDSNIDTEIKQINDMLKSLEDLNKSIDIISGSGSTPNDLLDERDRILDNL
                     SFKLDFENSDVKNMLADGKLELNELKNPDGTWKTGISGTLQGLFEMHGKIDTYKSDLK
                     DVSDGLAKQINDVYNTSAGITVTDFFITSNVDGEDIIKINPAIKSNPNELKLTADEAS
                     KIAKLKDEKIDIGVAGGKVSTISDHYKAFAESIGLDSQKVNQDEANQRKIINNIDNSR
                     MSVSGVSLDEEMTELIKIQRSYQASAKVMSTAVQLLDVVINGII"
     gene            21099..22031
                     /gene="flgL"
                     /locus_tag="PC471_001413"
     CDS             21099..22031
                     /gene="flgL"
                     /locus_tag="PC471_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HDF2788548.1"
                     /translation="MRVSTGMMSSSYLNSLQDNLQRLDKVNRQVNTTKEINKLSDNPY
                     KAIKILNSKSEIKTMETYVENCKDTGNWLETTDTALDQLGKLLSDIKKTLVASGDGAY
                     SDDELKTLSNETNEKMKEIANALNATHEGKYIFSGSNTGVPPVECIDNADGSVSLKFN
                     SSLNLNKLNDSLSMDIAQGISVDYNVKLSSLGFDPTKTDPFEELNNISKKLANPDADA
                     IKELTTTCLGDMEQLIENTVNVRSIYGTKANTVEAMKEKNDDSLIQLKDILSQNEEID
                     YGEKLVQLKAAELTYQASLQTGGKLFNVSILDYI"
     gene            22047..22439
                     /locus_tag="PC471_001414"
     CDS             22047..22439
                     /locus_tag="PC471_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliW"
                     /protein_id="HDF2788549.1"
                     /translation="MMKVTLKKGILGFENLKEYELLDIEDNDYLKEFNSTEEEGIGFV
                     VVSPFEIINEYEIVLNQETIEKLEVKSPKDVVLFNIITLGQTFEESTVNMKAPIVINI
                     KTNFGMQIILQDDKYSIRHPLLRGDNGC"
     gene            22433..22645
                     /gene="csrA"
                     /locus_tag="PC471_001415"
     CDS             22433..22645
                     /gene="csrA"
                     /locus_tag="PC471_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011987028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HDF2788550.1"
                     /translation="MLVISRKKDEAVLIGESIEVKIVGVDGNNVKLAISAPNNISILR
                     KEIYEKVKSENIKATNKNIKILKSLK"
     gene            22667..23062
                     /gene="fliS"
                     /locus_tag="PC471_001416"
     CDS             22667..23062
                     /gene="fliS"
                     /locus_tag="PC471_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HDF2788551.1"
                     /translation="MYGENPYNSYKQNAVFMASKEQLLLMLVDGAVKYTKIARAAILD
                     KNTQKAHRELVRVQDIFTELMVTLDQNAGQWAKDMYKVYDFVRYELSRANIRKDIQVI
                     DNVLPVIEQIKDTWHEADKKSKEERSRYK"
     gene            23062..23418
                     /gene="fliS"
                     /locus_tag="PC471_001417"
     CDS             23062..23418
                     /gene="fliS"
                     /locus_tag="PC471_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HDF2788552.1"
                     /translation="MELNLAELDKLSKEELLLMLVDGTVKYTNISKEALLNNDYLKAH
                     NELVRVQNIFTELMTTLDQNAGQWAKDMYKVYEFIKSELAIADENKDIKIIDDILPIV
                     KQIRDTWHEVYNQLKI"
     gene            23439..24962
                     /gene="fliD"
                     /locus_tag="PC471_001418"
     CDS             23439..24962
                     /gene="fliD"
                     /locus_tag="PC471_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HDF2788553.1"
                     /translation="MSSISPIRVTGLSGNFDMEGIIEASMIRDKEKVDKAKQEQQIVK
                     WKQEIYRNVIQESKDLYDKYLSINSPNSIVSEKAYSSTRITSSDESIIVAKGSAGAEK
                     INYQFAVSQMAEPAKFTIKLNSSEPIVRQFPPNASGASSLTIGDVNIPISEQDTTSTI
                     VSKINSLCADNDIKASYSEMTGELIISRKQTGSSSDINLRVIGNDNLAQQIANDNGIT
                     FINDAGGNKVANVYGKNLEADVTDEHGRVTHISKEQNSFNIDNIDYNVNSKGTAKLTS
                     VTDTEEAVKNMQAFVDDYNKLMDKVYGLVTTKKPKDYPPLTDAQKEDMTTEEIEKWEK
                     KAKEGILRNDDELRGFVEDIQSAFFGDGKNIIALRKLGINESENYNKKGQISFNADTF
                     SKALIDDSDKVYKTLAGYSSNYDDKGMFEKLKDIVYEYSGSSTSKLPKKAGIEKTASA
                     SENVYSKQIAEQERNISRLVEKMNDKEKRLYAKYSALESLLNQYSSQMNYFSQAQGN"
     gene            24965..25300
                     /gene="fliT"
                     /locus_tag="PC471_001419"
     CDS             24965..25300
                     /gene="fliT"
                     /locus_tag="PC471_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FliT"
                     /protein_id="HDF2788554.1"
                     /translation="MDKLKEKLNLYKDISLQIINLIEKEEYIKISIKLGERQEIINSV
                     SEIDRNDFIQLYNRMELIEIDSRIRDILQGQLLEVKKELHEYKLTKQVNTMYYNLNRE
                     KVNIFNKKV"
     gene            25402..26274
                     /locus_tag="PC471_001420"
     CDS             25402..26274
                     /locus_tag="PC471_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HDF2788555.1"
                     /translation="MRVNTNVSALIANNQMGRNVNAQSKSMEKLSSGVRIKRAADDAA
                     GLAISEKMRAQIKGLDQAGRNVQDGISVVQTAEGALEETGNILQRMRTLSVQSSNETN
                     TAEERQKIADELLQLKDEVERISSSIEFNGKKLLDGSSTEIRLQVGANFGTNVAGTSN
                     NNNEIKVALVNTSSIMSKAGITSSTIASLNADGTSGTNAAKQMVSSLDVALKELNTSR
                     AKLGAQQNRLESTQNNLNNTIENVTAAESRIRDTDVASEMVNLSKMNILVQASQSMLA
                     QANQQPQGVLQLLG"
     gene            26367..28508
                     /locus_tag="PC471_001421"
     CDS             26367..28508
                     /locus_tag="PC471_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDF2788556.1"
                     /translation="MLLSIVMIVKNEEKILEKTLKSLTTLRNSIESELIIVDTGSTDD
                     TIKISKKYTEKVYFHNWNNDFSSMRNISISYAKGEWLLILDADEILIDSSTIVKFFKD
                     GLDKKFNSASIQLKNLYSYDKKLYGYCSVLRLFKNVDFRYHGKVHEQPIYKNPIFNNV
                     ADFEHYGYLFEDEEIRLNKVKRNEKLLFQELKENDNSPYTNYQLGKNFIILGKYQEAL
                     YYLEKSYELYSKLELAPGYLITSLVKTYLYLGKHKKCERLCLKYIKKDMNNIDIYYYI
                     AQAQVDLGKYENSIDSYKRYIYLLDNYEISTQANSLFSDTDTVGLRDEAIITLIKIYY
                     KLEKYEFVISEYNNIEDVEKKKNVYFSLFMSLYKLNRFEDIKNYYKEVSNSKIEKNSF
                     YRNIEEVINNIKEDEKEYVYKILCDIEGNYGILNQIRINKCVSIDKCKEILNEEKEII
                     YAYIIKIAFEEKIDLLDIFYEMEYVWIEKYLKYLLALERSFNLVLYKYIISRPNTSDM
                     EKIRVYKAISKVVLESISLSDEKYKEIFYLYIMYSYKYIRYIYSNLSDFELIKFVCND
                     LDKFTIEFKHLLDIKNKVNNANEKLEYIHSMRKLLNEYPFYNKIIKILIGELEENIKE
                     SEEFKDLKDSFIISIENMIETGKINDAKLLVDDYSKTFVDDIKILNIKGILYMFENKL
                     DKADFMLKKALSLDVENEDTIYNIEYLKSLR"
     gene            28532..30409
                     /locus_tag="PC471_001422"
     CDS             28532..30409
                     /locus_tag="PC471_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDF2788557.1"
                     /translation="MNIPIVVNEKSKDIIKDWQYTCTNEDIKFTSIIILTYNQIEYTK
                     LCIESIRKFTPKNKYEIIIIDNNSSDGTVEWLKGQSDIKSVYNNKNLGFPKGCNQGIE
                     LATGENILLLNNDVIVTPNWLYNLNKALWSQDNIGAVGPITNACSYYQQIDVDYKSIE
                     DMFIFAHKINKSNINLWKYRLKLVGFCMLIKKTVLDNVGVLDELFTPGNFEDDDLSFR
                     MIESGYKLLLCQDTFIHHFGSVSFKENRNKYTDIMYINRRKFETKWGFNPEYSGMIRT
                     DLISKIDDSLEANINVLEIGCGIGATLLEIKNIYKNASIYGIEISESAGKLAKYSADV
                     LIANIEKVKLDYKKDFFDYIILGDVLEHLIDPWKIVNELKQYLKKDGYLVSSIPNIMH
                     VSVLRNLVLGNFTYEDSGILDKTHLRFFTLNEITKMFNDSNYIINSITATSMPLSEID
                     NSFIDTICKESCKEMRQQYESYQYIVKASKILDTKRYSSISMINLKYALMRIDNELDV
                     DSNLSYIFDNYNITEPCFVDDIEYLIDTFVINKATLLNRLGVEAFNRGLGDFSINMFM
                     TVLEMDRDDIDSIYNITSVLCELEEFDTAYNLIKNSSEKVQSESDIQEILCFIEGKKY
                     E"
     gene            30402..32516
                     /locus_tag="PC471_001423"
     CDS             30402..32516
                     /locus_tag="PC471_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HDF2788558.1"
                     /translation="MNNDKICFITCVNDERLYEECLVYINSLDIPKNFSIETVALRGA
                     DSIASAYNKAMRETDSKYKVYLHQDTFIINKNFIHDILEIFKKNTDVGMIGVAGVKDM
                     PVNGIWWDSDKKAGKVYDSHTGKMDIYEFSNFEECFNEVMAIDGLIMITQYDVFWRED
                     KFDGWHFYDLSQSMEFRQNKYKVIVPKQNKPWCMHDSGVVNINNGYDEYRIKFVKEYI
                     NLDLYRENFYEFDKKKASTILKSNKHVMDCMKEELTEQLRLKNYKDVLGIVSKIAHFS
                     VFNHTGLYSCEECERALLECANNLPEIENTKLFTKKDLKRRKVLHVLSEGYSTGGHTR
                     LVKNWIEIDKDSVHSVVTTWQSDTLPDWLLKAINDSGGEVISLDLISDDYLKRAVALR
                     KIAYDWADIVILYVHMWDTIPVVAFGVDGKIPILHVNHYDSTFWLGTSIADIVIDFSE
                     ASQLITLDKRGIDKSEILPMPLKEKEVFSKSEVRKRYNLEDDVTIILTIASERKYNSV
                     NDINYFKIAKTLIENTDNTIIIVVGVDTENLYWKTLSNVTDNRVICFGTTANIDDFYK
                     ISDIYMESFMVGSHTSKLDAILYDLIPVKFKNEVSPIFTNMWNFIDKFDYKNIKEVID
                     MIKRYNTSEFDRDRIKEKQSEIKHFVIDKHCIEIKNYIDKIYNEANHHKVKYDLYKND
                     EIEDYQLFVALYGYMVKNKNMS"
     gene            32525..33520
                     /locus_tag="PC471_001424"
     CDS             32525..33520
                     /locus_tag="PC471_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation glycyltransferase
                     FemA/FemB family protein"
                     /protein_id="HDF2788559.1"
                     /translation="MNNIHINKFEKSCERNWDYFVENKSINGTFLQTRNFLNYHEEGK
                     FTDHSLIICKGEEILAVVPACETYEDGERIFFSHLGSTFGGIIIRSGFNDIRHVNAIT
                     NKLEIYLREQNFDKVYLKNTSRIFCKEDTSLLEYFLFKNNYMFYDELSMFLELSKYKE
                     DILANFKYSKRRDFKYSLKNNLTFKRLYSDEDIVCFFNLLSNCLDSKGAKVIHSVEEL
                     LEFKNNRLKDIVEFYAVYIDDIMICGSMVFNFQERVYHTQYLASNSTYNKYYPMIFLY
                     YNLIKDALERNFKYLSFGISTLNRGKYLDEGLATFKEGFGSSAICNRTYYKNINK"
     gene            33612..34844
                     /locus_tag="PC471_001425"
     CDS             33612..34844
                     /locus_tag="PC471_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HDF2788560.1"
                     /translation="MKRLMILGSIKYFENIVISAKKEGIYTIVCDNRIDTPAKKICDE
                     AIDVDVFDFDKIKSIAIDKKIDGILTGFTDSLMKPYVYIANELNLPCVISCNQLESVT
                     NKAIMKKYFKNNAIPTTDYCIIESLKDLEKIQGLNFPLVIKPVDGYGSRGVYFVSNIE
                     ELIEKFEYSKEASQDGQVIIEEFYESEEIQGLAWVHNGESHVFYIGDRELVNIHTGRA
                     GKPDRLLYPSKYCFQYEEEIKSIYQNITQAFDIKNGPLYVQMLVGAYGIKVSEVMPRL
                     PGGCDYMAISQITDLDIGSLFANFSVNNEVNFDEIKKYNMHLSKCVYALPIYIRPGTI
                     KKINNIDKIENLDYVTQFLLLVKEGDTIELYEDERQDCGRVYGIANNIFDANIKKKCI
                     HEMIEILDENNENMIENF"
     gene            34864..35808
                     /locus_tag="PC471_001426"
     CDS             34864..35808
                     /locus_tag="PC471_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase"
                     /protein_id="HDF2788561.1"
                     /translation="MKLITHEQIMSLNISQKQGYEWIKESFLTKKDCQLPPKISMVEE
                     GHVFYNVMPSLNHNHMIGGIKMVTRLPGRNPSLNSEIFLYNMEDAKLKAILDGNYITA
                     FRTAAVAALAIDTFAKKDFNTIAIMGLGVTASAIMNMLSYTLKDKNINIKLLKYKDQA
                     ELFVKKYEKNEQFSFEICDTHEELIVDSDVVISTVTYTDEYLSEFKWFKKGALLLPVH
                     VMGFGNVDYLFDKVYLDDYNHLKHLENIDRYKYSCEFYDVLSKQAKGRENDDERIISY
                     NIGLSIHDILFANKIYELCETLSCEEIELKEPKEKYWI"
     gene            35902..36309
                     /locus_tag="PC471_001427"
     CDS             35902..36309
                     /locus_tag="PC471_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxcM-like domain-containing protein"
                     /protein_id="HDF2788562.1"
                     /translation="MDKYNLIRFVSIGNEYIGNLVALESNKVIPFDIKRVYYIYDVPN
                     NVKRGFHAHKNLEQVLVCLNGSVKIKCYDGVKETIFNLHKNDVGLYIGTGVWHEMFDF
                     DEKTVLISIASEHYNEEDYIRDYKEFLKYLNNV"
     gene            36396..37487
                     /locus_tag="PC471_001428"
     CDS             36396..37487
                     /locus_tag="PC471_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HDF2788563.1"
                     /translation="MLVKFNNLLKQFQTHKEEYEQKIKDVMESGYYILGNEVKHFEEE
                     FANYVGSKYCVGLASGLDALIIAFRALGISKGDEVIVQSNTYIASVMGISINGATPVF
                     VEPNEYYNIDSSKIEEKINSKTKAILVVHLYGQASNMKDIQELCDKYNLYLIEDCAQS
                     HGAKYDGKMTGTFGDIGCFSFYPSKNLGAFGDAGAIVTSNEEIADKVKILRNYGSEKR
                     YYNKFVGYNSRLDEIQAGLLRVKLKYLNEIEIEKSKIVDRYLNEINNPKVILPKIENN
                     CTHVWHLFVLQVEKRDTFMEYLKKSGIESIIHYPIPPHLSEAYAYLGYKKGSFNIAEN
                     YSDKVVSIPLFIGMEDEEVSYIIKIINEY"
BASE COUNT        15415 a         3788 c         6833 g        11532 t
ORIGIN      
        1 tgttagtttt tatgtataaa tctaacaaaa atatagttta tgattgcaaa tatcatattg
       61 tattttgccc taagtatcgt aaacctgttt tagttggcga agttgaaaag atgttaaaag
      121 aaatactacc atataaagca gatgaacttg gagcagaaat aatagaaatg gaaactgata
      181 aagaacatgt gcacttatta atatcttgtg accctcaata tggaattcat aaagtagtaa
      241 aaggtttaaa aggctttagt tcgagagtat taagagagaa ctttccacat ttaaagtcta
      301 gtatgcctag catgtggact aatagctatt ttgtaggtac tgttggttct gtgagtttag
      361 aagtagttaa acaatatata gaaaatcaac aagttaggac aaagaaatga taaaaactca
      421 tagagtgaaa ttaaacctaa ctagaacaca atttgaatta gtaagggaaa agcaaatgga
      481 aagtgctaac tgttggaact acatagttaa cttatctaaa gaatactatt ttgaacataa
      541 acaatggatt aaaaagaatg atatacaaaa gttaatcaaa ggtaagtaca atcttcatag
      601 tcaaactatt caagctatat cagataaatt tgatgcaaat agaaagacta tatcagaatt
      661 aagaaaaaaa ggtaatacta aagcaaaata tccatacaag actaagaaat tttatataat
      721 accttttaaa gctagtgcta ttaatagaaa tgctaaaggc aacttaaaat tatcaatgtc
      781 aaaaggcagg tatttagaat tagattttaa tgttgagaat ataaaaactg ctgaaatagt
      841 atggcgaaat ggatattatt tatactacac ttttgacaat gaattaaatg gagttgtagc
      901 caaaggcact aataccgtag gtgtagactt aggggaaata cattcgatag ctagtgtaac
      961 aaataaaggt gtagggctta ttctatctaa tagagaaggc agaagtatta aacaatttag
     1021 aaataagatg tatgcttata tatctaaaag attaagtaaa tgtaaaaaag gtagtagaca
     1081 atcaaagaag ttatggagat taaaaaataa gataagaagt aaaacagata atcagttaat
     1141 gaatttatat catcaaacaa ctagaaagtt tatagacttt tgtgtagagc agaaggtatg
     1201 tgaaatagtt cttggggata taaaaggggt tgaaaaagat actaagaaaa agaaaagatt
     1261 aaacagagtt aatagacaga aaatatctca aatggagtat ggtcgaataa aggactatat
     1321 aaaatataaa gcaaaagagc aaggtattga agttaagtta gttaaagaaa actacacttc
     1381 tcaaacttgc ccaaagtgtt ctaaaaaaca caaaccaact ggtagaactt atagttgtac
     1441 ttgtggttat gaaactcata gagatatagt tggtgcttgg aatattttaa ataaaaagca
     1501 taaatatggt ttagtagatt ttagaataaa tcataagcaa ccaataaatt taaaagtatc
     1561 taccgtttga gccatgtgtg ggcgaataac tttaaggtag tagtggcgaa gacaccgtcc
     1621 gaagtagctt ggctttgtaa ccacctctgt tgtactgttt aagtataaat aagtgtgtta
     1681 gccaccaaga atccccttgc tttagctatg gggagtgtgt caataaaata ctttaatgaa
     1741 aaatagctaa ctttaattgt aggatattct taatatattt gaaataaata aaaatattga
     1801 aggatataat attcaactgg tatataaata ataaatgata gttaaatttt accttagagg
     1861 ctaattaggt gtttagtcaa agctaatgcc tttagaatac aaatgtattc taaaggcatt
     1921 tttactatgt attttcattt atattactaa acattatcta tttttatata catttctatg
     1981 tattttattt tcatcaaatc ggtctataaa ctcatttttt tcttcactat atccaataga
     2041 tataagtgca taaggaacta attttaatgg taagtctaaa gtttgtctaa ctttatttac
     2101 cactttattt ttaggaaata ctcctgccca acaagcagct agaccttgat tagtaacttc
     2161 taataataaa ttttgtgtgc aagcacctaa atcttgtatc catttaccag gttttaagaa
     2221 tttatctcta tttccaagca ctactataca taatggtgca aattctatat gctttgctct
     2281 atgctgtgac tttgagagct gtttcagcag ttccttatca tctactataa taaattccca
     2341 aggttgagca ttttttgatg aaggtgcttg catagctgat tttagtattt cagttatttt
     2401 atcttcttct atatttttat ctatatatgt tcttattgaa cgtctatttt ttataacttc
     2461 taacatacag attctccttt aaataaaatt atttctattt atataatatc attactttaa
     2521 attttagcta ttacttatat aaagaataaa ctttgtgttg gttttatcta agtaaaagta
     2581 acattcagaa ctaaaaaaca agactataaa tccaaagtat taattaaaaa agaagtaata
     2641 aaaaataata tacaagattt aaaaaagagt attgggctta aaatttaatt agaaaaccaa
     2701 ttttgagata atctgttttt tattttaaca aaaaaacata aataaagttc gatacgtagc
     2761 gaatgaaaaa attacaagaa atattttttt taaaaaattt caaaaaagag ttgataatat
     2821 atgtatatta atataaaatt taaggtgtat aattcgtata ataatacgaa tgatttgtag
     2881 tgtaaaataa cttataaacg gagaggggaa gtctaatgaa agtagcagta gttatgggtt
     2941 caaaatcaga ttatccaaaa ttagaagaag ggataaaact gcttgaaaaa tatggaatag
     3001 aagttgtagc aagagcatta tcagctcata gaacaccaga acaactttca atattcctaa
     3061 aagaaataga agatgatact gatgtaataa tagcagcagc aggaaaagca gcacacttac
     3121 caggtgtaat agcttcacaa acattaattc ctgtaatagg attacctatt aaatcatcaa
     3181 ctatggatgg actagactca cttttatcaa tagttcaaat gccaaaagga atcccagttg
     3241 caacagttac aatagactta ggtttaaacg cagctttatt ggcattacaa ataatgactt
     3301 taaaataccc aaaattaaaa gaggatttga aatcatatag agaagagatg gcacaaaagg
     3361 tgttagaaga cgacaataat ttaagggggt agcagaaatg ttattatatg aaggaaaagc
     3421 aaaacaagta tattctacag ataatgaaaa tgaatatgtt gtttattata aagatgatgc
     3481 aacagcattt aatggagaaa aaaaagcaga aatatcttca aaaggtatac taaataataa
     3541 gatatcaaca ataatctttg aaatgttaaa agaaaataat ataaatactc actttataaa
     3601 aagtttatca gatagagaaa tgttagttaa aaaagtagag attttaccat tagaagtaat
     3661 agtaagaaat attgctgctg gttctatctg taaaagagtt ggtcttgaag aaggtgtagt
     3721 tttcgatgag ccaatttttg aaataagcta caaaaatgat gcttatggtg acccaatgct
     3781 aaatgatgat tatgcagtgg caatgaagtt agcaactaga gaagagttaa aattcttaag
     3841 agaagaaact ttaaaaataa atgagttact aaaagcattt ttcttaaaat taaacctaaa
     3901 gttagttgac tttaaaatag aatttggtaa ggattctgaa ggaaatataa tattggcaga
     3961 tgaagtatca ccagacactt gtagactttg ggatgtaaat acaaatgaaa agttagataa
     4021 ggacagattt agaaaagacc ttggagacct tgtagaaggt tatacggaag tactttcaag
     4081 aatgaataat aaataggagg caaatgtatg tgtggagttt tagggattta ctctaataaa
     4141 gatgtaacta aagaactata ttattcttta tattctatgc aacacagggg gcaagaaagc
     4201 tgtggtttag ctcttttaga tgatggagaa attaagtaca aaaaagatat gggattagtt
     4261 ggagatgttt ttaaggaaaa tgaattatct aagttaaaag gaaatatagg gattggacat
     4321 gttagatatt caacagctgg aggaagtcat gtttctaatt gtcaaccttt agttggaagt
     4381 tgtagaaaaa gacaattagc catagctcat aatggaaatc tagttaatgc aaattattta
     4441 aaagatatgc tagaagaaga tggatatatg ttccaaacta attcagatac agaagtgatt
     4501 ttatatatac ttgctagata ttacaaaggg gatatagtag aaagtttaaa agtgacaatg
     4561 gattatataa aaggtgcata tgctcttgta ataatgagtc aagaagaatt ggttgcagtt
     4621 agagacccac atggattcag acctcttgta cttggtaaaa agggtgatga atatatattt
     4681 gcatcagaaa attgtgcaat agatatactt ggcggagaag ttataagaga tgtagagcca
     4741 ggagagataa tagtagtcaa agatggagaa ctaaaatcat atttttattc agaaaattat
     4801 aaaccagtta agaaaagttg tatatttgaa cacatatact ttgcaagaaa tgatgcaact
     4861 atagacaatg taaatgcata tgaatttaga attaaatgtg gtgagagatt agctcagaat
     4921 gaaactgtaa aggcagatat ggtagttcca gttccagatt caggttggcc aggagctatt
     4981 ggttatgcaa atgctagtgg attaaaaata agtgaaggct tagttaaaaa tagatatgta
     5041 ggaagaacat ttataaaacc tacacaagaa gaaagagaaa tagcagtaaa aataaaatta
     5101 aatcctcttt caacaataat aaaaggaaaa tctataattt tagtagacga ctcaattgta
     5161 agaggaacta catctaaaca attagtaaaa tctttaagag aagcaggtgc aaaagaaatt
     5221 catcttagaa taacttctcc tccagtagct tactcttgtt actatggaat agatactcca
     5281 aatcgctcta aattaattgc atcaagtaac aacgtagaag aaatgagaga atatattgga
     5341 tgtgatagtt taaaattcct agatatagaa ggaatgttag atgcaacaga acataagtct
     5401 actttctgta aagcttgttt tgatggagaa tacccagtta agaaaattga taaggaggaa
     5461 ctactttcat gttaacatat aaagaatcag gtgtagatat agatgaagga aatagagcag
     5521 tagaccttat aaaaggtaaa ataaaaggaa cttatgatgg aaatgtagtt ggagatttag
     5581 gtaacttcag tggattatac agtttaaaag attttgtagg tatgaaagaa ccagttttgc
     5641 ttgcatctac agatggtgtt gggactaagt taaaaatagc tcagatgatg gacaagcatg
     5701 atactgttgg aatagattta gttgcaatgt gcgtgaatga tttaatttgt caaggagcaa
     5761 aaccattatt cttccttgac tatatagctc ttgggaaatt agtgcctgag catatagaaa
     5821 aaatagttgg tggaattgca gatggttgca aaatgtctgg ttgtgcacta attggtggag
     5881 aaactgctga aatgcctgga atgtatggag aagatgacta tgatttagca ggattttctg
     5941 tgggaatagc agacaaagaa aagattgttt ctggaaacaa tgttaaatca ggagatgtat
     6001 tggttggtat atcttcaagt ggagtacaca gcaatggatt ttcttttata agaaagatat
     6061 ttttagaaac ttacaattat aaaatggaac gatatgttga agaactagga atgacagttg
     6121 gagaagcact tttaacacca actaaaatat atgttaaatt agctttagat gtattagcta
     6181 aacatgacat aaaagctgta gctcacataa ctggtggagg gcttatagaa aatataacaa
     6241 gagttatacc taaaggatta ggactagata tcaataaaaa atcttgggag aagcctccta
     6301 tatttaaaat gatagaaggt tttaatgctg ttgatgagag agaacttcat aagagtttta
     6361 acatgggtat tgggctagtt ttaatagtag ataaagaaaa tgctgatgat gttgtaaact
     6421 ttataaataa tagagaaaat gacaatgctg attatgtaga taaaaaatat agtgaattat
     6481 tagaagataa agcttatata attggtgaag tagtagatag tcatgaaggt gtagaattat
     6541 gctaaatata ggagttttaa tatctggtgg gggaacaaat ttacaagcag ttatagatgg
     6601 tactgagtct ggagaaataa aaggtcaggt taaagtagtt atctcaagta agcaaggtgc
     6661 ttatgggcta gagagagcta aaaatcataa cataaaagca atttgtgaaa ctgatgaaga
     6721 caagataata gaaattctta aggaaaataa aatagattta gtagttttgg ctggttattt
     6781 aaaaataatc agcccaaagc tggttaatga gtttagaaac aaaatgataa acatacatcc
     6841 atcattaatt ccttcatttt gtggagctgg attctatgga gaaaaagttc atcaaggtgt
     6901 tatagattac ggagctaaag taactggtgc tacagttcat tttgttgatg aaggtgctga
     6961 tactggacca atcataatgc aagatgtagt taaggtaaat caagatgatg atgcaaagac
     7021 acttgcaaaa agagtgttag aagttgaaca taggatttta aaagaaagta taagtctttt
     7081 ttgtgaaaat aaattaaaac ttcaaggtag gagagtgttt ataaatgagt aagagagctt
     7141 taataagtgt aactgacaag actggtgtag tggaatttgc taaagagtta aataaattag
     7201 attatgaaat tatatctaca ggaaatacat ttaaaacatt aaaagaaaat ggtgttaatg
     7261 taatgcaagt tgaagatgtt acaaattttc cagaaatatt agatggaaga gtaaaaacat
     7321 taaatccata tatacatggt ggtatacttt ataaaagaga taaagaatct catgtagaaa
     7381 ctgtaaatga acataaaata cattcaatag atttagtagc tgtaaatcta tatgattttg
     7441 aaggaacttt aaaagctgga aaaagtcatg atgaaataat tgaaaatata gatattggtg
     7501 gaccatctat gatacgttct gctgccaaaa actataagga tgtaatagta gtagtagata
     7561 taaaagatta tgattctata atagaaaaat taaaaacaga tacaatgaca ttagaagaca
     7621 gaaaaaaatt atcatataaa gctttctcaa ctacaggaag atatgatgcg ttaatatcta
     7681 gttattttgc tggagaagtt ggagatacgt atcctgacat attaaattta actttccaaa
     7741 aagaacaaac tttaagatat ggagaaaatc cacatcaaaa tggtttctta tattcacagt
     7801 caaatgcaaa aaatccaatc ttaaattatg agcaattagg cggtaaagaa ctttctttta
     7861 acaacttaaa tgatttacat ggatgtttag aagtaatgag agaatttaaa gatagtgaag
     7921 aagtagtttc agtagctata aaacatgcta attcatgtgg ggtaggttta ggaaaagatg
     7981 cttttgaagc atatacaaaa tgttatgaag cggataaggt ttcaatattt ggaggaatag
     8041 taggaataac ttctactata gataaagcta cagcagagaa attaaatgaa atattcttag
     8101 aaatagtagt agcatatgat tttgaaccag aagctttaga gatacttaaa caaaagaaaa
     8161 atctaagaat attaaaatta gctaaaatag aaaatagttt acaaccatat gaaatgaaat
     8221 atttagatgg aaaattattg atacaagata gaaacaatat attagcagaa aaaagtgaaa
     8281 atgtaacaaa ggaaaaacca acagatgcac aacttaagga catggaattt ggtatgagag
     8341 tagtaaaaaa tatgaaatca aatgcaattg caatagtaaa aaatggacaa actctagctt
     8401 taggttgtgg acaaacatca agaatttggg cattaaaaaa tgctcttgaa aataacaaag
     8461 ataaagattt tacaggagct gttttagcgt cagacgcatt tttcccattt gatgattgtg
     8521 taacattagc tcatgaatat ggaatatcag cagtagttca accaggtggc tctataaaag
     8581 ataaagattc aatagaagct tgtgacaagt atgatatggt tatggtattt acaggaataa
     8641 gacacttcaa acactaagga ggatattatg aagatattgg ttgtaggcgg tggcggaaga
     8701 gagcatgcta tatgctggaa actaagcaaa gaaaaaaatg tagaaaaaat ttattgtgca
     8761 ccaggtaatg caggaattgc aaacgtagca gaatgtgtaa atataggtga tactaatata
     8821 gaagaacttt taaaatttgc caaagaaaat gaaataggtc tgacaatagt aggaccagaa
     8881 gtaccactag ttatgggaat agtagatgag tttgaaaaag aaggattaag agtatttgga
     8941 cctaataaaa aatgtgctca acttgaagga agtaaagctt tttcaaaaga atttatgata
     9001 aaacataaca tacctactgc aaaatataaa gaatatacaa acttagaaga agctatatca
     9061 gaaatagata gttttggata tccagtagta ataaaagcgg acggattagc tgctggaaaa
     9121 ggtgttgtaa tacctgaaaa tagagaagat gctatagcaa ctttaaaaga aatgatgtca
     9181 gataagaagt ttggagctgc tggagataaa atagtaattg aagagttttt aaaaggaata
     9241 gaaacttcaa ttctagcatt tgtagataat gatacaatag ttcctatggc aagtgcaaaa
     9301 gaccataaaa aagtaaacaa ctatgagcaa ggtccaaaca ctgggggaat gggaactttt
     9361 tcacctagtg aaatatatac agaagaatta gcaaataaag taaaagagac agtattagaa
     9421 aaaactttag aaggcttcaa aaaagatgga cttaatttta aaggtatttt atttgttggt
     9481 cttatgataa ctgaagatgg agaaaaagta cttgaatata atgtaagatt tggagaccct
     9541 gagacacaat ctgttttatt tagattagaa acagatttac atgagataat ggaagctata
     9601 ctagataata agttaaaaga tatagaaata aactatagtg atgatgaagc tgtatgtgtt
     9661 atgcttacat caggagggta tccagacagt tatgaaaaag ggaaaattat aactggtctt
     9721 gaaaacttgg atgatgatat agtagtattc catagtggca ctaagatgtt tgatggtaat
     9781 ttggttacca atggaggaag agtaattggt ataactgcaa agtcaactac agttaaggat
     9841 gcagcagaaa aagtttatga aaacataaag aagataaact ttgaaggaat gcactataga
     9901 acagacatag gaagataatt tttctaaaat ggaggataag gtatgttgaa tactgaaaat
     9961 aaagactcaa tggttagaag agtattagtt gaaaaaagag aaggttttga ccttgaggct
    10021 aaagctttaa aaaaagactt agtagaaagt ttacacatag ataatataga aaatttaaga
    10081 atattaaata gatatgatgt agaaggaata tcagaagaag tttatgaaaa tgcagcaaag
    10141 acaatattct ctgaaccaaa tttagatgta gtatattatg aagaaatacc aaaattaaat
    10201 gatgaaagag tatttgcaat agaatttctt cctggtcaat acgaccaaag aggtgattgg
    10261 gcagctcaat gtgtacaaat tgtaaatcaa gggataagac ctgctattaa tacagcaaaa
    10321 gtttatatat tatcaggcaa aataactgat gaagagtttt ctaaaataaa agattactgt
    10381 ataaatccag tagatagtag agaagcatct ttagaaaaac cagaaacgtt aaaaatggaa
    10441 actgaaatac caacaacagt tgaagtatta gatggattta tagatttaga tgaaaatgga
    10501 ttaaaaacat ttgtaagtga aaaaggatta gcaatgactt taggagactt acaacacgtt
    10561 caaaaatact ttaaagatac agaaaagaga aatccaacta taacagaaat aaaagtatta
    10621 gatacttatt ggtctgatca ttgtagacat actactttta tgactgaaat agaaaatgta
    10681 aagatagaag atggtaagtt taatgatata gtaaaagaag cgtatcaaat gtatcttaac
    10741 tcaagagaca atgtatatgt gaacagacat aaagatatat gtttaatgga tatagccact
    10801 gttgcagtaa aagaattaaa gaaaaatggg aaattaaatg acctagatga aagtgaagaa
    10861 ataaatgcat gtagtataaa cgtagatgtt gaagttgatg gtaagatgga aaaatatcta
    10921 gtaatgttta aaaatgaaac tcacaaccat ccaactgaaa tagaaccatt tggtggagca
    10981 gctacttgtt taggaggagc tataagagac ccattatcag gaagaagtta tgtttatcaa
    11041 gcaatgcgtg ttacaggaag tgctgaccca agaacgactt tagaagatac tttacctgga
    11101 aaacttatgc aaagaaaaat aactacagaa gctgctcatg gatatagttc atatggtaac
    11161 caaataggtc ttacaactgg acaagttgca gaagtatatg atgaaaactt tgttgcaaag
    11221 agaatggaaa taggtgcagt tatagcagca gcaccaaaag agaatgtagt aagagaaaga
    11281 cctgaagcag gagatgtaat cgttcttctt ggaggaaaaa caggaagaga tggctgtgga
    11341 ggagcaactg gttcatctaa agaacatagt gaagagtcaa tcttaacttg tagtgcagaa
    11401 gttcaaaaag gagatgctcc taacgaaaga aaaatacaaa gattctttag aaataaagaa
    11461 gttgctcaaa tgataaaaag atgtaatgat tttggagcag gtggagtttg tgttgctata
    11521 ggagagattg ctgatagctt agatataaac ttagacttag ttcctaaaaa atatgatgga
    11581 cttgatggaa ctgagcttgc aatttcagag tcacaagagc gtatggcagt agccataaaa
    11641 aaagaaaata aagataaatt tatacaatta gcagtagaag aaaacttaga agctacgcat
    11701 gtagctacag taacagatac aggatattta agaatgttct ggaatggaaa agcaattgta
    11761 gatataaata gagagttttt agatacaaac ggagtaaaac aaactactga tgttcatgtt
    11821 actaaagtag atgaagaaaa tacattcttt agctctaatg aaatagtaaa agatgttaag
    11881 tgtgcaagta tgaaagacaa gtttacaaaa gtactatctg accttaatgt ttgttctcaa
    11941 aaaggattag tagagatgtt tgataataca attggtggta atacagtact tatgccattt
    12001 ggaggaaagt atcaagcgac tccaacacaa ggtatggttg ctaagatacc agtacttgga
    12061 ggagaaacta atacttctac aataatgact tatggataca atccaaaggt aggaaaatgg
    12121 agcccattcc atggtgcctt gtatgcagta gtagaatctg tgtgtaaatt ggttgctatt
    12181 ggaggaaatt atagtacaac aagacttaca ttccaagagt attttgaaaa acttggtaac
    12241 aatccagaaa aatggggtaa accattctca gcacttttag gagcatttta tgcacaaagt
    12301 aaatttgaga tacctgctat aggtggaaaa gacagtatgt ctggtacatt taaagatatt
    12361 gaagttccac caacattagt ttcatttgct gttgacacag tagatgcgaa gaaagtagta
    12421 tctccagaat ttaaaaaagc agactcaaaa gtagtaatgt tatgtgtaaa caaggcagaa
    12481 aatgatgttg tagattttga ggaattaaag agaaacttag ataaagtaag agaacttata
    12541 catggaaata aagttttatc aacatatgca ttaggatttg caggtgtagg tgaagcaata
    12601 agtaaaatgg cttttggtaa taaaatagga tttaagttta gtgaagaggc tgaaaaagca
    12661 tttacagatg ataaattatt tgaggctagt tatggaaata tagtacttga attagcaaat
    12721 gatgatttaa gtatgttaga aggatacaat tatgttgtgc ttggaagtac agttaaagaa
    12781 actagtatat ttatcaaagg tgaagaactt gcattagatg aattatataa agcacattgt
    12841 agtactttag agcctatatt cccaactaaa acagaagaag taaaatcaaa gatagaaact
    12901 ataagttata tatcacaagg agaagctaaa aaatcatcat taagtatagc tactccaaga
    12961 gtatttatac cagcattccc tggaacaaac tgtgaatatg actctgcaag agcatttgaa
    13021 agagcaggag ctaatgcaag tataagagta tttaagaatt taacttataa agacatagaa
    13081 gattctatag atacaatagt aaacgaaata aaatcttctc aaataataat gttaccaggt
    13141 ggatttagtg ctggtgatga gccagatggt tctggtaaat ttatagctac tgtatttaga
    13201 aatcctagag tgcaagaagc tataaatgaa tttttaactc aaaaagatgg actaatgtta
    13261 ggtatctgta atggattcca agtattaatt aaattagggt tagttccata tggagaaata
    13321 agagttccaa gtgaaagtgc tccaacactt acttacaata atattggtcg ccatcaagct
    13381 aagattgcta gaactagaat ttcatctaat aaatctcctt ggttagctca aactaatgtt
    13441 ggagatattc ataatatagc aatttctcat ggagaaggta agtttgttgc cagtgaagat
    13501 gttatgagag aattaattgc taatggacaa gtagcaactc aatatgttga ctttaataat
    13561 gaagctactt atgatattga gtttaatcca aatggttcat tctatgctgt tgaaggtata
    13621 actagtgctg atggtagagt gtttggtaaa atgggacatt ctgaaagaat tggtgaggaa
    13681 gtatataaga atattatagg tgaaaaagac caaaagatat ttgaatctgg agttaaatat
    13741 tttagataaa gctttgatgt ttaattgata gctagaatat ataatttaga tatttagatt
    13801 taaagagatg taataatatg atgataatca tttaatatta catctctttt ttatttatct
    13861 aagaaataat aagttcaacc taagttaaaa tacaagctgt gttataatta attattaagt
    13921 aaaattgaat atttattgga ggacaacata attaatgaaa atattaataa cagggtctaa
    13981 tggtcaatta ggaaaagaat tggttaacca attagaagca atcaatcaaa gtataaacca
    14041 accaaaatat gttatattag ctacaacaag aagtgatttg gacatatcca atcagactaa
    14101 tgtagataat tttattttgc ataataaacc tgatgtagtt gtaaactgtg cagcatatac
    14161 aaaggttgat gcatgtgaag ataatatcga aattgcatac aaaataaatg ctttaggagt
    14221 gagaaatcta gcaatagcat cagaaaaagt taatgctaag cttattcaca tatcaactga
    14281 ctatgtattc aatggttttt ctaaatatcc atatagggaa gacaataaaa ctgaacccaa
    14341 ttctgtatat ggaaaaagta aacttatggg agaaaaattt gttgaacaat tctcacataa
    14401 gtactttata ttgagaacag catggttata tggtaatggg aataattttg taaagaccat
    14461 gataaaactt tctttagaaa ataaagaggt taatgtggtt aatgaccagt ttggaactcc
    14521 aacaagtaca gtagacttag caaaaattat aataaaaatc atggaaacag aacattatgg
    14581 agtttatcat gctacatgtg aaggtgagtg tagttggtat gattttgcca aaaagatatt
    14641 taaactaaaa aatatagata taaaagtaaa tccaattaag agtaacgaat ttaaatgtaa
    14701 ggctccaaga ccactatatt ctgtattgga taattttatg ttgaagttaa ttggtttaaa
    14761 ttcttttaga aagtgggaag aatccattga agaatattta gaaagatatg atataaatga
    14821 aaagtaaatg tattattttg agaaaagaaa ataagatatt ataaaaaata taaatatttc
    14881 ttttatttat ccgataatca taatatgaca aaaacaaatt taaggatgga aaaagatgaa
    14941 aggaattatt ttagcaggag ggtctggtac aagactatat cctataacaa aatgtatatc
    15001 aaaacaaaca cttccaatat acgataaacc aatgatatat taccctatgt cggttcttat
    15061 gttggcaggg atacaagata tattaataat atctacacca agagatataa aagcatttga
    15121 agaattattt ggagatggaa gttcactagg gcttaacata acatatgcaa ttcaggagaa
    15181 agcaaatgga attgcagaag cgtttataat aggagaagaa tttataggaa atgacagagt
    15241 gtctcttgta ttaggggata atatatttta tggatatgga tttagtgaaa ggttggagaa
    15301 tgcagttaaa agaaaaggag caactatatt tgggtatcat gtaagtaatc caaattcgtt
    15361 tggagttgta gaatttgata aggattttaa tgtattgtca atagaagaaa aacctacagt
    15421 tcctaaatct aattatgcta taccaggatt atatttttat gataatgatg ttgtagagat
    15481 agcaaagcta gttgaaccgt cagatagagg tgaattagaa ataacctcta taaacagtga
    15541 atatctaaaa cgaggaaaac taaaagtgga acttctagga agaggtatgg cttggcttga
    15601 tactggtaca cataaaggtc tattggatgc ttctaattat gttgaagtgg ttcaaacaag
    15661 acagggactg tatatagcat gtttagaaga gattgcatat acaaaaggat atataagtag
    15721 agaacaatta atgaaactag ccaagccact gataaaaact gaatatggtc aatatctatt
    15781 aaaattggct agtgaagata tgtaatatgg tagataaaat ttattataaa ttttattaag
    15841 ggagtttatt ataatgggaa aaatcaatgt tataaagact aaaatagaag gattatatat
    15901 agtcgaacca aatgtatttt atgatgaaag aggatatttt atggaaagct ataataaaaa
    15961 ggattttata gagcagggtc ttgatatgtg ttttattcaa gataatgagt ctagctcaag
    16021 aaaaggcgtt ttaagaggaa tgcattttca ggttaaatat cctcaaggaa aattagtcag
    16081 agttacagag ggagaagttt tcgatgttgc agttgattta aggaaagatt cacaaacatt
    16141 tgggaaatgg gaaggtgtaa tattgtctga taagaataaa aaacaatttt atattccaga
    16201 agggtttgca catggatttt tagttttatc agaaaaagca gtatttagtt acaaatgtac
    16261 aaattattat tacccagaat ttgagaatgg ctttatatgg aatgacaagc aaatatctat
    16321 tgagtggcct ttaaaaggaa ttgaagaaat atttttatca gaaaaggaca aaaaacaaaa
    16381 aagttttaaa gatatttgta caagacgtta ataaaaattt attagaatta acatttagga
    16441 gaaaaaatat gaaaaaaata ctgataactg gaggagcagg atttattggg agtaatttta
    16501 tacattatat gcttaacaag tatgtagatt attttattgt taacctagac ttgttgactt
    16561 atgctggtaa tttagagaca ttaatagaaa tacaagacaa ttccaattat aaatttgtta
    16621 aaggagatat ttcaaatagg gagcaggtgt ataagctatt tgaagaagaa aggtttgata
    16681 tagttgttaa ctttgctgct gaatctcatg tagataggtc agttgagaat cctgatttat
    16741 ttattaaaac aaacattata ggaacacaag tacttttaga tgcatctata aaatatggag
    16801 ttaaaaggta tcatcaaata tctacagacg aagtgtatgg tgatttacca attgatagaa
    16861 aggatttatt ctttacagaa caatcttcta tcaatccaag ttctccatat tcagcttcta
    16921 aagcatcagc agatttattg gtaagttctt attatagaac atatggattg ttaacaacta
    16981 tatctaggtg ttccaacaat tatgggccat atcattttcc tgaaaaattg attcctctta
    17041 tgattttaaa tgctttggaa aacaaacaat tgccagtata tgggaatggg gaaaatgtaa
    17101 gagattggtt acatgtatat gaccattgta ctgctataga cttaataata cataaaggaa
    17161 atatagggga gatttacaat ataggaggtc ataatgaaag aagtaatcta gaagtagtaa
    17221 aaatgatatt aaatctttta ggtaaatctg aagagttaat atcctatgta aatgatagac
    17281 ctggtcatga tttaagatat gctattgatg caactaaaat agagaatgag ttaggttgga
    17341 aggcaaaata tgactttgat ttgggtataa aagaaactgt taagtggtat atagaaaatg
    17401 aatcttggtg gaaagctgtt ttatcagtat aactttaaaa tttagaaagg ggcgttttat
    17461 attgaatgag ataagttcac taattaatat attagcattg actcaattat ctggtagtaa
    17521 tactacaaat caatgtaatt gtacatataa taattcatct ggatttgata tgattatgat
    17581 gaccttgtta aaagcattat cacaaaataa tcaacatact tctaatagct acaatttatt
    17641 gtctaattct gagaatttat ttaatgaact tgataatgta gtaaataata ccactagtag
    17701 atttatagat gttgatacaa aagataaaaa tgtaaagttg agaattgaaa atgctgtaaa
    17761 tcaagcatca aaaaaatata acgtagattc aaatcttata aaagcaataa ttaaagttga
    17821 atcagatttt aatccaaata ccgtttcttc tgctggtgct aagggtctta tgcaacttat
    17881 gccagaaaac tgcagagact taggggttac agaccctttt aatatagaac agaatataga
    17941 tgctggaact agacatatta aggaatacat tgatatgttt ggagggagca tagaaatggg
    18001 attgatggca tataatggtg gtcctggaag aatgaaaagt agaggagtag aatcaatctc
    18061 tgacctgtat aaaatgccaa aagaaacaca aaattatatt cctaaggtta tgaaatatta
    18121 tagagggtag gctttattat gggaataaat atagataagc taaacataag tccaagtata
    18181 aatatggagg ttaaaaaact aaaagagagt aataaatttt ctgacaaatt aaactcttta
    18241 aaacaatctt cttcattgga agatataaaa cgaaatttgg aaagtgtaaa aaaggcagga
    18301 gaaaggcttg tacttactca aaactatggc gatattagta tatataaaaa tgcagtaaaa
    18361 gaatacctaa aatctgttgt agataatatg tattcacttg ataaaaatag tagcttttgg
    18421 gagcatcagt attataagaa tgtaaagatt gttgatagta aacttgagga tatgactagt
    18481 aagttaatta gtgaagaaaa agaaaacata gatattgttt ctactgttga catgatacaa
    18541 ggcttattga tagatatgta tagataaggt tatacaagga gaaaaatttt atgaatagca
    18601 aaaaagagga tatatatgaa ttgaattttg ggcaattaat agaagaaaaa tctggtgtta
    18661 tagaagaaaa tcaaaatgtt tttaatgcaa aagtaaaggt agctgtttct ataggaaata
    18721 caacagaaac aataaaaaat atattagatt taaaagaagg ctctataata aaattaagta
    18781 agaatgtgga tgaaaaatta gatatatatg caaacgatag attgtttgct tatggagaaa
    18841 gcattattac aaatgagaag ttatctgtaa gattatctaa aatagaagat tcagatgtaa
    18901 attagcattt taaatatatc cctttagtct attctgtatg agtttagcta aggggatatt
    18961 tttataaaaa atttaaaata tgtgtgaatt atataaatat ttttatttta tgagcgataa
    19021 ctaaattata agtatgtaat tttggaggtg aaactatgga tattaaaaat gtaagttcaa
    19081 atattgtagt gaataattat ttaaagaata catctggtat aaaaaaagtg gaagaggtta
    19141 aaaaaattaa taaaacagac aagatagagt tatctaaatt tgcaaaggaa atggcaagtg
    19201 tgtcaaatat gagtgatgag aatattagaa ataaaaaagt cgatgaaata aaaaatgcat
    19261 tagaaaatgg aacttacaaa ataaaaccaa aagaaattgc gaaaaaaatg atagaggata
    19321 tgcgaggata aagtcatgat tacagactta aaagttataa tatatgaaga aaaaaatata
    19381 attagtaaga tgatggaatt attaaaagaa caatttgatt atatagttga aaaagactta
    19441 gaaaatttaa atatgttaaa tcctaaacta gaagatatta gcagggagct tgcatccata
    19501 gaaataaaga gaagacaact ttttggtaat gatatttcaa tgagtgaagt tgttgaaaat
    19561 agtaatgatg gatatttaaa agaaatatat acagacttga aacgcattct tgctttagct
    19621 aaaaatcaac aagaatcaaa tgattctcta ataaagaaag aacttatttt tacgaaaaaa
    19681 atgattaatt tcataaaacc agctgataag caaactacaa cttataattc ttatggaaat
    19741 attagaaaat aaatattagg aggagaaata tggcaggatt attttccatt ttaaatacag
    19801 ctaattcagg aatgaatgcg caccaaaaag caatacagac aatttctcat aatataagta
    19861 atctagatac agacggttat tctaggcaaa ggaccgagtt tgctacaaat agtccaatgt
    19921 atatgcctag tttgagtaat tcaattgcaa gaggacaatt agggactgga gtacatgtaa
    19981 cggatgtaac aagagctaga aatagttttt atgattacca atttagggca gaagctcata
    20041 agtatggtaa gacagtttca aaatatgatt attacaatac tatagaaact atactaaacg
    20101 aaccatcaga ctatggaata tctgcaggta tagatgattt ttttaaagga tgggagtcat
    20161 tatcaaagga tataaatagt gacagtaaaa aaagtctagt ggtagaaaac gcatctaatt
    20221 tagcaacttt aataagtgaa agttataaga agcttgataa gttaaaagaa gatatagatt
    20281 ctaatataga cactgagata aaacaaataa atgacatgtt aaaaagtctt gaagatttaa
    20341 ataaatctat tgatattata tctggttctg gctctacacc aaatgatttg cttgatgaaa
    20401 gagatagaat tttagataac ttgagtttta aactagactt tgaaaatagt gatgttaaaa
    20461 atatgcttgc agatggtaaa ttagaactta atgaattaaa aaatccagat ggaacttgga
    20521 aaacaggtat atcagggacc ttacaaggct tgtttgaaat gcatggtaag attgatacat
    20581 ataaaagcga tttaaaagat gtatctgatg gattagctaa acagataaat gatgtttata
    20641 atacgtctgc aggaataaca gtaacagatt tttttataac ttcaaatgta gatggtgaag
    20701 atattataaa gataaatcca gctataaaga gtaacccaaa tgaattaaag cttactgctg
    20761 atgaagcttc aaaaattgct aagttaaagg atgaaaaaat agatattggg gtggctggtg
    20821 gaaaagtaag tacaataagt gaccactata aagcctttgc agaatcaatc ggtttagatt
    20881 ctcaaaaagt aaatcaagat gaagcaaatc aacgtaaaat tataaataat attgataact
    20941 caagaatgag tgtttcaggt gtatctttgg atgaagaaat gacagagctt ataaaaatac
    21001 aacgttcata tcaagctagt gcaaaagtta tgtcaacagc tgtgcaacta ctggatgttg
    21061 taataaatgg aataatttaa tagaaggggg aatttataat gagagtcagt acaggtatga
    21121 tgtctagcag ctatctaaac tcactacaag ataatctaca aagattggat aaggttaata
    21181 gacaagttaa tacaacaaaa gaaataaaca agctatcaga taatccatat aaagcaataa
    21241 agatattaaa ctcaaaaagt gaaataaaga ctatggagac ttatgttgag aattgtaaag
    21301 atacagggaa ttggttagaa actactgata ctgcattaga tcagcttgga aaattattat
    21361 cagacataaa gaaaactttg gtggcaagtg gtgatggtgc atactcagat gatgaattaa
    21421 agactcttag taatgaaact aatgaaaaaa tgaaagaaat tgcaaatgct ttaaatgcaa
    21481 cacatgaagg gaagtacata ttttcaggtt caaatacggg ggtaccacct gttgaatgta
    21541 tagataatgc tgatggttca gtgagtttaa agtttaattc tagtctaaat cttaataaat
    21601 taaatgactc tttgagtatg gatatagctc aaggaataag tgtagattat aacgttaaat
    21661 tgagtagcct tggatttgac ccaacaaaaa ctgacccatt tgaagagtta aataacatat
    21721 caaaaaaatt agcaaatcca gatgctgatg ctataaaaga gcttacaaca acttgtttgg
    21781 gcgatatgga acaactaata gaaaatacag taaatgttag gtctatatat gggacaaaag
    21841 caaatacagt agaggctatg aaggaaaaaa acgatgatag tttgatacag ttaaaggata
    21901 ttttgtcaca aaatgaagaa atagattatg gtgaaaaact tgttcaatta aaggcagcag
    21961 agttaactta tcaagcttca ctacaaacag gaggaaagtt atttaatgta tctatattag
    22021 attacatata ataaaataac gcagatatga tgaaggttac attaaaaaaa ggtatattag
    22081 gttttgaaaa tttaaaagaa tatgagcttt tagatataga agataatgat tatttaaaag
    22141 aatttaattc tactgaagaa gaaggtatag gatttgttgt tgtatctcca tttgagatta
    22201 tcaatgaata tgaaatagtt ttaaatcagg aaacaataga aaaactagaa gtgaaatctc
    22261 caaaggatgt tgtattattt aatataatta ctcttggtca gacatttgaa gaatcaactg
    22321 tgaacatgaa agctcctata gtgataaata taaaaactaa ttttggaatg caaataattt
    22381 tacaggatga taaatattca ataaggcatc cattattgag aggtgataat ggatgctagt
    22441 aatttcaaga aaaaaagatg aagcagtact tataggagaa agcatagaag taaaaattgt
    22501 aggagtagat ggaaacaatg taaaactagc tatatcagcg cctaacaata taagtatatt
    22561 aagaaaagaa atatacgaaa aagtaaagag tgagaatata aaagctacaa ataaaaatat
    22621 aaaaatttta aagtcattaa aataataagc ttggaggaag taatatatgt atggagaaaa
    22681 tccttataat tcttataaac aaaatgctgt ttttatggca tcaaaggaac aattgttact
    22741 tatgcttgtt gatggagcag taaaatatac caaaatagcc agagcagcta tcttagataa
    22801 aaatacacag aaagcgcata gagaacttgt aagagttcag gatatattta cagaactaat
    22861 ggttacatta gaccaaaatg caggacaatg ggcaaaggat atgtacaaag tttatgattt
    22921 tgtaagatat gaactttcaa gagccaatat tagaaaagac atccaggtaa tagataatgt
    22981 gttacctgta atagaacaaa ttaaagatac atggcatgaa gctgataaaa aaagtaaaga
    23041 agaaagaagt aggtacaaat aatggaatta aatttagctg aattagataa gttatcaaaa
    23101 gaggaattac tattgatgct tgtagatggc acagtaaaat ataccaatat atccaaagaa
    23161 gctcttttaa ataacgacta tttgaaagca cataatgaac ttgtaagagt tcaaaatata
    23221 tttacagaac taatgactac attagaccaa aatgcaggac aatgggcaaa ggatatgtat
    23281 aaagtttatg aatttataaa atctgaatta gcaatagctg atgaaaataa agatattaaa
    23341 ataattgatg atatattgcc aatagtaaaa caaattagag atacatggca tgaagtttac
    23401 aatcaactaa aaatataatt agatggaggg ataaaggtat gtcaagtata agtcctataa
    23461 gagttacagg tctttcagga aattttgata tggaaggcat aatcgaagct agtatgatta
    23521 gagacaagga aaaagttgat aaagcaaaac aagaacaaca aatcgttaaa tggaagcaag
    23581 aaatatatag aaatgttata caagaatcaa aagatcttta tgataaatat ctaagcataa
    23641 attctcctaa tagtatagta agtgaaaaag catactcttc tacaagaata accagttctg
    23701 atgaaagtat tatagtagca aaaggttcag ctggtgcaga aaaaataaat tatcaatttg
    23761 cagtttctca aatggctgaa ccagcaaaat ttactattaa attaaattca agtgaaccta
    23821 ttgttcgaca gttccctcca aatgccagtg gagcgagttc tttaactata ggagatgtaa
    23881 atataccaat atctgaacaa gatactacaa gtactattgt aagtaagata aactcccttt
    23941 gcgcagataa tgatataaag gcttcttata gtgagatgac aggtgaattg attatttcaa
    24001 gaaaacagac tggttcgtca tcagacatta atttaagagt aattggaaat gacaatttag
    24061 ctcagcaaat tgctaatgat aatggtatca catttataaa tgatgctggt ggaaacaaag
    24121 tggcaaatgt atatggaaag aatctagaag ctgatgtaac tgatgaacat ggaagagtaa
    24181 ctcatataag taaagaacaa aattcattta atatagataa tattgactat aatgtaaatt
    24241 caaaaggaac tgcaaagttg acttctgtca ctgatactga agaagctgtt aaaaatatgc
    24301 aagcatttgt ggatgattat aataaactga tggacaaggt ctatggttta gttactacta
    24361 aaaaaccaaa agattatccg cctcttacag atgcccaaaa agaagatatg acaactgaag
    24421 aaatagaaaa atgggaaaag aaagctaaag aaggtatact tagaaatgat gatgagttaa
    24481 gaggttttgt tgaagatatt caatctgcat tttttggaga tggaaaaaat attattgcat
    24541 taagaaaact aggtatcaat gaaagtgaaa attacaataa aaaaggtcaa atatcattta
    24601 atgcagatac tttttcaaag gctcttatag atgatagtga taaggtatac aaaacactag
    24661 caggttattc ttcgaattat gatgataagg gaatgtttga aaagctaaaa gatattgtat
    24721 atgaatattc tggaagttca acttctaaac ttcctaaaaa agcaggtata gaaaaaactg
    24781 cttctgctag tgaaaatgta tattcaaaac aaattgcaga gcaagaaaga aatataagca
    24841 ggttagttga aaaaatgaat gataaagaga aaagacttta tgctaaatat tcagccttag
    24901 aatctttgtt gaatcagtat tcttcccaaa tgaattattt ctcacaagca cagggtaatt
    24961 aattatggat aaattaaaag aaaaattaaa tttatataaa gatatatctt tacaaattat
    25021 aaatttgatt gaaaaggaag aatatataaa gataagtatt aagttaggtg aaagacaaga
    25081 gattataaat agtgtaagtg aaattgatag aaatgatttt atacagcttt ataatagaat
    25141 ggaattaatt gaaattgata gcaggataag agatatttta caaggacaat tattagaagt
    25201 aaaaaaagaa ttacatgagt ataagcttac taaacaagtg aacacaatgt attataactt
    25261 aaatagagaa aaagtaaata tttttaataa aaaagtataa agttatgtac catcttgtcc
    25321 gattatataa atgaagaatt aataaaaaag attaaaagta gaaatgacaa ggatgtcaac
    25381 tatactaagg agggtaaaat aatgagagtt aatacaaatg taagtgcttt gatagcaaat
    25441 aaccaaatgg gaagaaacgt aaatgcacaa agtaagtcta tggagaagct atcttctggt
    25501 gttagaatta agagagctgc tgacgatgct gctgggcttg ctatatctga gaaaatgaga
    25561 gctcaaataa aaggtttaga ccaagcagga agaaacgttc aagatggtat atctgttgta
    25621 caaacagcag aaggtgcatt agaagaaact ggtaacatac tacaaagaat gagaacttta
    25681 tcagtacaat cttcaaatga aactaatact gctgaagaaa gacaaaagat agctgatgag
    25741 ttactacaat taaaagatga agttgaaaga atatcaagct caattgagtt taatggtaag
    25801 aaattacttg atggaagttc tacagaaata agattacaag ttggagcaaa ctttggaaca
    25861 aatgttgcag gaacatctaa caacaacaat gaaataaaag ttgctctagt aaatacttca
    25921 agtataatga gtaaagcagg tataacaagt tctacaatag caagcttaaa tgcagatgga
    25981 acttctggaa caaatgcagc taaacaaatg gtatctagct tagatgtagc tcttaaagaa
    26041 ttaaatacat caagagcaaa attaggggca caacaaaata gactagaatc aactcaaaac
    26101 aacttaaata atactataga aaatgttaca gcagctgaat caagaataag agatacagat
    26161 gttgcttcag aaatggttaa cttatctaaa atgaatatat tagttcaagc atcacagtca
    26221 atgcttgctc aagctaatca acaaccacaa ggagttttac aattattagg ataaaataat
    26281 tttgtatatt ggatatagaa gaaagggtaa ggctttgctt tactcctttc tttttttaca
    26341 aatatacttt tgaaaggaaa atttatatgt tacttagtat agttatgatt gttaaaaatg
    26401 aagaaaaaat acttgaaaaa actttaaaat cattaaccac attaagaaat tctatagaaa
    26461 gtgaacttat aatagtcgat acaggttcta cagatgatac tataaaaata tctaagaagt
    26521 acacggaaaa agtgtatttt cataattgga ataatgattt ttctagtatg agaaatatat
    26581 caatatccta tgctaaaggt gaatggttac ttatattaga tgcagatgaa atactaattg
    26641 attcttcaac tattgtaaaa ttttttaaag atggtcttga taaaaaattt aattctgctt
    26701 caatacaatt aaaaaatctt tactcatatg ataaaaaatt atatggatat tgttcagtgc
    26761 ttagactttt taaaaacgtt gattttagat accatggtaa agtacatgaa caaccaatat
    26821 acaaaaaccc tatatttaat aatgttgctg attttgaaca ttatggatat ttgtttgaag
    26881 atgaagaaat aagattaaat aaggtgaaaa gaaatgaaaa actattattt caagagttaa
    26941 aagagaatga taattctcca tatacaaact atcaattagg gaaaaatttt attatattag
    27001 gaaaatatca ggaggcttta tactatcttg agaaatctta tgaattatat tctaaactag
    27061 aattggcacc tggttacttg ataacaagcc ttgtaaaaac atatttatat ttaggaaaac
    27121 ataagaagtg tgaaagattg tgcttaaagt atattaagaa agacatgaat aatattgata
    27181 tatattatta tattgcacaa gcacaagtag atttaggaaa gtatgaaaat agtatagatt
    27241 cgtataagag atatatttac ttgttagata attatgaaat aagtacacaa gcaaatagtt
    27301 tattttcaga cactgatact gtagggctta gagatgaggc tattattaca ctgataaaga
    27361 tttattataa gcttgaaaaa tatgagtttg taatttcaga gtataataac attgaagatg
    27421 tggaaaagaa aaagaatgtg tactttagct tatttatgtc actatataaa ttgaatagat
    27481 ttgaagatat aaaaaattat tataaagaag tttcaaattc taaaattgaa aaaaatagtt
    27541 tttatagaaa tatagaagaa gttataaata atattaaaga ggatgaaaaa gagtatgtat
    27601 ataaaatcct ttgtgacatt gaaggtaatt atggcatttt aaatcaaata aggataaata
    27661 aatgtgtatc tatagataaa tgtaaagaaa tactcaatga agaaaaggaa ataatttatg
    27721 catatattat aaaaatagct tttgaagaga agattgattt actagatatt ttttatgaaa
    27781 tggaatatgt atggatagag aaatatctaa aatatctttt agcattagaa agaagtttta
    27841 atttagtact atataagtac ataattagtc gtccaaatac atcagatatg gaaaagataa
    27901 gagtatataa agctatatct aaagtagttt tagaaagtat aagcttaagt gatgaaaaat
    27961 ataaggagat tttttatctt tatataatgt attcttataa gtacataaga tacatatata
    28021 gcaatttaag tgattttgaa ttaataaaat ttgtttgtaa tgatttagat aagttcacta
    28081 tagaatttaa gcatctactt gatattaaaa ataaagtaaa taatgctaat gaaaaattag
    28141 agtatataca tagtatgagg aaattactaa atgagtatcc tttttataat aaaataataa
    28201 aaatacttat aggtgaatta gaagaaaata taaaagaaag tgaagaattt aaggatttaa
    28261 aagatagttt tattattagt atagaaaata tgattgaaac tggcaaaata aatgatgcga
    28321 aattattggt agatgactat tcaaaaacat ttgtggatga tataaagatt ttaaatataa
    28381 agggaatttt atacatgttt gaaaataagc ttgataaagc tgattttatg ttaaaaaagg
    28441 cattatcatt agatgtagaa aatgaagata ctatatataa tatagaatac ttaaaaagtt
    28501 taagataagt caaaaatagg agaattgaag aatgaatata ccaatagtgg ttaatgaaaa
    28561 atctaaagac ataataaaag attggcaata tacatgtaca aatgaagata taaaatttac
    28621 aagcataata atattgacat ataatcaaat agaatatact aaattatgta ttgaaagtat
    28681 taggaaattt accccaaaga ataaatatga aataataata attgataata actcttctga
    28741 tggaacagtt gagtggttaa agggacaatc tgatataaaa tctgtgtata ataacaaaaa
    28801 cttaggattt ccaaaaggat gcaatcaagg tattgagtta gctactggtg aaaacatatt
    28861 gttgttaaat aatgatgtaa tagttactcc aaattggctc tataatttga ataaagcttt
    28921 atggtctcaa gataatatag gtgctgtagg acctattacg aatgcatgtt catattatca
    28981 gcaaattgat gttgattata agtcaattga ggacatgttt atatttgcac acaaaataaa
    29041 taaatctaat attaacctat ggaaatatag attgaagtta gttggatttt gcatgttaat
    29101 aaaaaaaact gttctggata atgtaggggt tttagatgaa ttattcacac ctggaaattt
    29161 tgaagatgat gatttatctt ttaggatgat agagtcagga tataagttat tattatgcca
    29221 ggatactttt atacaccatt ttggaagtgt gtcttttaaa gaaaatcgta ataaatatac
    29281 agatataatg tatattaata gaagaaaatt tgaaactaag tggggattta atccagaata
    29341 tagtggaatg ataagaacag atttgatttc taaaatagat gatagtttgg aggcaaatat
    29401 taatgtttta gaaataggat gcggtatagg ggctactctt ttagaaataa aaaatatata
    29461 taaaaatgca tcaatatatg gaatagaaat tagtgagtca gcaggtaaac ttgcaaagta
    29521 tagtgcagat gtacttatag caaatataga aaaagtaaaa ttggattata aaaaagattt
    29581 ttttgattat ataatattgg gagatgtact tgaacatttg attgacccat ggaagatagt
    29641 caatgaattg aaacaatatc taaagaagga tggttatctg gtatctagta taccaaatat
    29701 tatgcatgtt tctgttttaa gaaatcttgt gttaggtaat tttacatatg aagattcagg
    29761 aattttggac aagactcatt taaggttttt tacattaaat gagataacaa aaatgtttaa
    29821 tgatagtaat tacataatta atagtattac agctactagt atgccacttt cggaaattga
    29881 taatagcttc attgatacaa tatgtaagga aagctgtaaa gaaatgagac aacagtacga
    29941 atcttatcaa tatatagtaa aagcctctaa aatcctagat actaaaagat attctagtat
    30001 tagtatgatt aatttaaaat acgctttaat gagaattgat aatgaattag atgtagatag
    30061 taatttaagt tatatatttg ataattataa tataactgaa ccatgttttg ttgatgatat
    30121 tgaatatcta attgatacat ttgtgataaa taaagcaact ctattaaata gattaggagt
    30181 tgaggcattt aacagaggtc taggagattt tagtataaac atgtttatga cagtattaga
    30241 gatggataga gatgatattg actcaatata taatataaca agtgtattat gtgaattaga
    30301 agaatttgat actgcttata atttaattaa aaatagtagc gaaaaagtac agagtgaaag
    30361 tgatatacaa gagatacttt gttttattga ggggaagaaa tatgaataat gataagatat
    30421 gttttataac ctgtgtaaat gatgaaaggc tttatgaaga atgtcttgta tatataaata
    30481 gcctagacat tcctaaaaac tttagtatag aaacagtagc tttaagagga gccgattcta
    30541 ttgcctctgc ttataataaa gctatgagag aaacagattc taagtataag gtatacttac
    30601 atcaagatac ttttataatt aataaaaatt tcatacatga tattttagag atatttaaaa
    30661 aaaatacaga tgtcggaatg attggtgtag ctggagtaaa agatatgcct gtgaatggaa
    30721 tttggtggga ttctgataaa aaagcaggga aagtatatga tagtcataca ggtaaaatgg
    30781 atatttatga atttagtaac tttgaagaat gttttaatga agttatggct atagatggat
    30841 taattatgat tactcaatat gatgtatttt ggagagagga taagtttgat gggtggcatt
    30901 tttatgattt gtcacagagt atggagttta gacaaaataa atacaaagtt attgtaccaa
    30961 aacaaaataa gccttggtgt atgcatgatt ctggagtagt aaatataaat aatggatatg
    31021 atgaatatag gataaaattt gtaaaagagt acataaatct tgatttatat agagaaaatt
    31081 tttatgaatt tgataagaag aaagcaagta ccatacttaa gagtaataag catgtaatgg
    31141 attgtatgaa agaggagctt acagaacaat taagattaaa gaattacaag gatgttttag
    31201 gtattgtttc taaaattgct catttttctg tttttaatca tactggtttg tattcttgtg
    31261 aagaatgtga gagagcattg ctagaatgtg caaataattt accagaaata gaaaatacga
    31321 aattatttac aaaaaaagat ttaaaaagaa gaaaagtatt acatgtttta agtgaaggat
    31381 attcaacagg aggtcataca agactagtta aaaattggat tgaaattgac aaagactctg
    31441 ttcattcagt tgtaactaca tggcaatcag atacactgcc agactggtta ttaaaagcaa
    31501 taaatgatag tggtggcgaa gttatatctt tggatttaat ttcagatgat tatttaaaaa
    31561 gagctgtagc tttgaggaaa atagcttatg actgggctga tatagttatt ttatatgttc
    31621 atatgtggga tacaatacct gttgttgctt ttggagttga tgggaaaata cctattttac
    31681 atgttaatca ctatgatagt actttttggt tagggacaag tattgcagat attgtcattg
    31741 attttagcga agctagtcaa ttaattacat tagataaaag aggcatagat aaatctgaaa
    31801 tattaccaat gccattaaaa gaaaaagaag tattttcaaa atctgaagtt agaaaacgat
    31861 ataatttaga ggatgatgtg acaataatat tgactattgc tagtgaaaga aaatacaata
    31921 gtgtaaatga tattaattac tttaaaattg ctaaaacatt gattgaaaat acagataata
    31981 caattattat tgttgtagga gttgatacag agaatctcta ttggaaaact ttaagtaacg
    32041 ttacagataa tagagttata tgttttggaa caacagcaaa tatagacgat ttttacaaga
    32101 tttcggatat atatatggag tctttcatgg taggaagtca tacttcaaaa ctagatgcaa
    32161 ttttatatga tttaattcct gttaagttta aaaatgaagt ttcaccaatt tttacaaata
    32221 tgtggaattt tatagataag tttgattata agaatataaa agaggttata gatatgataa
    32281 aaaggtacaa tactagtgaa tttgacaggg ataggataaa agaaaaacaa tcggaaataa
    32341 aacattttgt tatagataaa cactgtattg aaataaaaaa ctatatagac aaaatatata
    32401 atgaagctaa tcatcataaa gttaagtatg atttatataa aaatgatgaa atcgaggact
    32461 atcagctttt tgtagcatta tatggctata tggtaaaaaa taaaaacatg tcataggtgg
    32521 taaaatgaac aatattcata tcaataaatt tgaaaaaagc tgtgaaagaa actgggatta
    32581 ttttgtagaa aataaatcta taaatggtac atttctgcaa actagaaatt tccttaatta
    32641 tcatgaggag ggaaaattca ctgaccactc attaataata tgtaaaggtg aagaaatact
    32701 agcagtcgta cctgcatgtg aaacttatga agatggtgaa agaatatttt tttctcattt
    32761 agggagtact tttggtggta ttataataag aagtggtttt aatgatatta ggcatgtaaa
    32821 tgccattaca aataagttag aaatatattt acgggaacaa aatttcgata aagtatattt
    32881 aaaaaataca tcaagaatat tttgtaaaga ggatacttca ttactagaat attttttatt
    32941 taagaataat tacatgtttt atgatgaatt aagtatgttt ttagaattaa gtaagtataa
    33001 agaagatata ttagccaatt ttaaatacag taaaagaaga gattttaaat attctttgaa
    33061 gaataatctt acctttaaaa gactgtatag tgatgaagat atagtttgtt tttttaattt
    33121 actttctaat tgtttagata gtaaaggagc taaagtaata cattcagtag aagaactttt
    33181 ggagtttaaa aataatagat taaaagatat tgtagaattt tatgcagtat atattgatga
    33241 cattatgatt tgtgggagca tggtatttaa ctttcaagaa agagtttatc atactcaata
    33301 tctagcatca aattcgacat ataataaata ttatccaatg atatttttat actataatct
    33361 aataaaagat gctttagaga gaaattttaa gtatctttct tttggaatta gcactctaaa
    33421 tagaggcaaa tacttagacg agggtttagc tacatttaaa gaaggttttg gttcaagtgc
    33481 aatttgtaac agaacatact ataaaaatat aaataagtga gaatcatgtt ttatagagtt
    33541 tttagaatat agtaaatatc tagtactcta acattacata aaaataatta gtttataaag
    33601 aaggtttgta tatgaaaaga ttaatgattt taggctcaat taagtatttt gagaatattg
    33661 ttatatcagc taaaaaagag ggaatatata cgattgtttg tgataataga attgatactc
    33721 ctgcgaaaaa gatttgtgat gaagcaatag atgttgatgt atttgatttt gataaaataa
    33781 aaagtatagc tatagataaa aagatagatg gaattttaac tggatttaca gattctttaa
    33841 tgaaaccata tgtttatatt gcaaatgaac taaatttacc atgtgtgatt tcttgcaatc
    33901 aacttgaatc tgtaactaac aaggctatta tgaaaaaata ttttaaaaat aatgcaatac
    33961 ctacaactga ttactgtatt attgaaagtc taaaagattt agaaaaaata caaggattaa
    34021 attttccact tgtaataaaa cctgttgatg gttatggttc aagaggtgta tattttgtat
    34081 caaatataga agaattaata gaaaaatttg aatattctaa ggaagcctca caagatggac
    34141 aagtaataat tgaggaattt tatgaaagtg aggaaataca aggactagct tgggttcata
    34201 atggagagtc acatgtattt tatattggag atagagagct tgtaaacata catacaggaa
    34261 gagctggaaa accagataga ttactttatc catcaaagta ttgttttcaa tatgaggagg
    34321 aaataaaatc tatatatcaa aatataacac aggcttttga tataaaaaat ggacctcttt
    34381 atgtacaaat gttagtggga gcatatggaa taaaggtatc tgaagtaatg ccaagattac
    34441 ctggaggttg tgactatatg gctatttctc aaataacaga tttagacata ggaagtttat
    34501 ttgcaaactt tagtgtgaat aatgaagtaa attttgatga aataaagaaa tataatatgc
    34561 atctatcaaa atgtgtatat gcattaccaa tatacataag acccgggaca ataaaaaaga
    34621 taaataatat agataaaata gaaaacttag attatgtcac tcaatttcta ttactagtaa
    34681 aagaaggaga tacaatagaa ttatatgaag atgagagaca ggattgtgga agagtttatg
    34741 gaatagcaaa taatatattt gatgcaaata taaaaaagaa atgtattcat gaaatgatag
    34801 aaatacttga tgaaaataat gaaaatatga tagaaaattt ttaaataagg gagtatgata
    34861 gatatgaagc taataactca tgaacaaatt atgtcattaa acatttctca gaaacaagga
    34921 tatgagtgga taaaagaatc tttcttaaca aaaaaagatt gtcaattacc accaaaaata
    34981 agcatggtag aagaaggaca tgtattttac aatgtaatgc catccctaaa tcataatcat
    35041 atgattggtg gaataaaaat ggttacaaga ttgccaggga gaaatccaag tttaaactca
    35101 gagatatttc tatacaatat ggaagatgct aaattaaagg ctattttgga tggtaattat
    35161 attacagctt ttaggactgc tgctgttgct gccttggcta tcgatacatt tgctaaaaaa
    35221 gattttaata ctattgctat tatgggttta ggtgtaactg cttctgctat aatgaatatg
    35281 ctttcatata ctcttaaaga taaaaatata aatataaagt tacttaaata taaagaccaa
    35341 gcagagttgt ttgtaaaaaa gtatgaaaaa aatgaacaat ttagttttga aatatgtgat
    35401 acacatgaag aacttattgt agattcagat gtagttattt ctactgtgac atatacagat
    35461 gaatatttat cagaatttaa atggtttaaa aaaggtgctt tattattacc tgtacatgtt
    35521 atgggatttg gaaatgttga ttatctattt gataaagtat atttagatga ttataatcat
    35581 ttgaaacatc tagaaaatat agatagatat aaatattctt gtgaatttta tgatgtttta
    35641 agtaaacaag ctaaaggaag agaaaatgat gatgagagaa taatatctta caatataggt
    35701 ttgtcaatac atgatatatt atttgcaaat aaaatttacg aattgtgtga aacactaagc
    35761 tgtgaagaaa tagaattaaa agaaccaaaa gaaaaatatt ggatatagtt tatttcattc
    35821 atatttacaa tagtttattg taaatatgaa tatgaagtaa tatattgtta gaaaattagc
    35881 atgaatagga tagataaatt tatggataaa tataatttaa ttaggttcgt atctattgga
    35941 aacgaatata taggaaattt agttgcatta gaatcaaata aagtaatacc ttttgatata
    36001 aagagagttt attacatata tgatgtacca aacaatgtaa agagaggatt tcatgcacac
    36061 aaaaacttgg aacaagtact agtttgccta aatggaagtg tgaagataaa atgttatgat
    36121 ggtgtaaaag agactatatt taatttacat aaaaatgatg ttggattata tataggaacg
    36181 ggagtgtggc atgagatgtt tgattttgat gaaaaaactg ttttaatttc tatagcatct
    36241 gaacattata atgaagaaga ttatattaga gactataaag aatttttaaa gtatttaaat
    36301 aatgtgtagt tattatacaa ttagtgatgt attttcttaa ttaaataaat ttaagatagc
    36361 cacatagaga atagatagat taggatgtga atttattgtt ggttaaattt aataatttat
    36421 taaaacagtt ccaaactcat aaagaagaat atgaacagaa gattaaggat gtaatggaaa
    36481 gtggatatta tatccttgga aatgaagtaa agcattttga agaagaattt gctaactatg
    36541 taggtagcaa atattgtgtt ggtttagcca gtggtttaga tgcactgata attgctttta
    36601 gagcattagg aataagcaaa ggtgatgaag taatcgtgca atcaaataca tatattgcta
    36661 gtgttatggg aatcagtata aatggagcaa caccagtatt tgtagaacca aatgaatatt
    36721 ataatataga ttcatctaag atagaagaaa agataaatag caaaacaaaa gctatattag
    36781 tagtgcattt atatggtcaa gcaagtaata tgaaagatat acaagaatta tgtgataagt
    36841 ataacttgta tttaattgaa gattgtgctc aatctcatgg agctaaatat gatggaaaaa
    36901 tgacaggaac atttggtgat ataggttgtt ttagttttta tccaagtaaa aatttgggtg
    36961 catttggaga tgcaggagct atagtaacta gtaatgagga aatagctgat aaagtgaaaa
    37021 ttcttagaaa ctatggctca gaaaagcgat attataataa atttgtcgga tataattcaa
    37081 gattggacga aattcaggca ggtttattaa gagtaaaact taaatatcta aatgaaattg
    37141 aaattgaaaa atcaaaaatc gtagatagat atcttaatga aattaataat cccaaagtca
    37201 tacttccaaa gatagaaaat aattgtactc atgtatggca tttatttgtt ttacaagttg
    37261 aaaaacgaga tacatttatg gaatacttaa agaaaagtgg tatagagtct ataatacatt
    37321 atccgatacc accacatcta tctgaagcat atgcttattt gggatataaa aaggggagtt
    37381 ttaatatagc tgagaactat tcagataaag ttgttagtat acctttattt attggtatgg
    37441 aagatgaaga agttagttat ataattaaaa ttataaatga atattaaact actcataata
    37501 atatcaaaaa taaatttttt atatatcatt tatttaaatt tcgttaattg acatttttaa
    37561 atattgat
//
LOCUS       DAMJYR010000030        35619 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.030, whole genome shotgun sequence.
ACCESSION   DAMJYR010000030 DAMJYR010000000
VERSION     DAMJYR010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 35619)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35619)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35619
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.030"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            131..271
                     /locus_tag="PC471_001429"
     CDS             131..271
                     /locus_tag="PC471_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2788564.1"
                     /translation="MGIIESASKLAEMVHLLAVEKGITDIEAWDEAVKEYSKIYEERR
                     NE"
     gene            complement(446..766)
                     /locus_tag="PC471_001430"
     CDS             complement(446..766)
                     /locus_tag="PC471_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HDF2788565.1"
                     /translation="MKLKTISLPELNNLDPTLESTFIKMGEEQGELAECIGKFRNLSG
                     ENNDLDEVDIIKKTAKELMDVAQTCVTMMFKLEEQYGINLDEIRKEHIKKLEKRGYIK
                     NMDK"
     gene            complement(886..1623)
                     /locus_tag="PC471_001431"
     CDS             complement(886..1623)
                     /locus_tag="PC471_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HDF2788566.1"
                     /translation="MDANSLKDLIANHNNIVFFGGAGVSTESNIPDFRSSTGLFSQKL
                     NKQFTAEQLVSHTFFVRYPEEFFEFYKDKLIYPNAKPNNAHIALAKLEEMGKLKAVIT
                     QNIDGLHQMAGSKNVLELHGSVHRNYCTKCGKFFDLESMLNLGGNIPYCDNCGSIVKP
                     DVVLYEEALDSDVITKTISAISNADLLIIGGTSLAVYPAASFIDYYKGDYIALINKAN
                     TVYDKSASLVINKPIGEVLYEAVLRQI"
     gene            1838..3661
                     /gene="acd"
                     /locus_tag="PC471_001432"
     CDS             1838..3661
                     /gene="acd"
                     /locus_tag="PC471_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosaminidase"
                     /protein_id="HDF2788567.1"
                     /translation="MKKKAALATLAMLPLGVVNAHADGDIGIVTINYLNVRNEPTAES
                     SIAFVAKKDDKVLIKDSSNGWYKIKAESGQEGWASSKYIAKSNSDSLRTSTNKEKQVI
                     SNSLNMRNGAGTSYRVITVLKKGQKVEVISESNGWSKIKYDGRLGYVSSSYLGDVSNS
                     TNKSKTKQVNTTSLNVRSGPNTSYGLLGKLPKGSKVEVISEGNGWSKIKYNGKDAYVS
                     SMYLSDVSQSNSDNSSQSNDKKNTDKVVNTASLNVRSGPGSTYSKLGKVYKGSKVTVL
                     SESSGWAKINFNNKEAFVVGNYLSTSADTSNNNSNSNSDNSSNSNGNNSSSSGQVNGM
                     SGISGAKIDYKSLSYTLESHISKQVEKAASGGNVIAPSNRKSTPSPEFSTFSAQRTSS
                     FVNASSSDIEYYLNPKNFTNTTKGMMQFLKINSYRDGISESSLNSYLNGLSSSVFKNQ
                     GAAFINAAKKYNIDVVYLVSHAMWETAYGKSTLAQGQTLTSYKGQPLSKPVKVYNFFG
                     IGAIDKSANVSGAEAAYSNGWTSVEATIDGSAKWISQNYVNSSKYNQNTIYKMKWNYD
                     YTWHQYATDVNWANGISGIMENLIGLYGGGSSLVFEVPQYK"
     gene            3876..8174
                     /locus_tag="PC471_001433"
     CDS             3876..8174
                     /locus_tag="PC471_001433"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HDF2788568.1"
                     /translation="MESIKEYLDKLEINNSGLGKQLKEVYINRVVYFKEDKIVYFYLT
                     SKDIVSHELLDKFKEELMYKLDYFKDMRMKIRFTGLERKSNKDVIKKYWNNILYILKY
                     LCPSIAGWYKQVEFLCLEEELKIKLPKGIFYERLMKKNVTYVLKTVLSEELGLDLNIT
                     IEKAVDEKVNKERLIRINDREMEEKIRALEIGKVNNCEENEEESYVIKSDVDENLIYG
                     DNANAMIENIVELNASSGTVAVVGDIFDVDTKELKNGKILMIASITDYTSSISCKLFL
                     TDTNKDGVLENVKKGAYLKIKGDIVYDTYQREISMMISGIRKETRIEREDKSEEKRVE
                     LHAHTQMSSMDAICSVKKLVERAAKWGHSAIAITDHGVVQGFPDAMNAGKSNNIKILY
                     GVEGYLVEDDSLIIEDANDKELSQTFVVFDIETTGFSNTNDKITEIGAVKIENFKIVD
                     RFSELINPEKDISYKIQELTGITNELIKDKPTIEEVLPKFMEFVGDSVLVAHNAEFDT
                     GFISQKCREQGLLYNNKKVDTLMLARVMLPNLKRHRLNVVAKELGIPLLNHHRAVDDA
                     EATALIFNKFLQMLTEKGAKTLSDVNNILGKIDYTKLSTNHITLIAKNSIGIKNLYKI
                     ISDAHVNHFFRAPRILRSVLNEYKEGLIIGSACEAGVVFQAVKKNVSDEEMKKIIDLY
                     DYIEVMPIDNNRFMIDKGEVKDEEELRELNRKLIDTAKKFDKIPVATGDVHFLDKHEA
                     VLRKVLKYSQGFKVDEEETYLHFRTTDEMLEEFSYLGEELAYEVVVENSNLIADMVED
                     IKPIPDDTYPPIIEGSDVELREMCYKKAKRIYGDPIPEIVQKRLDRELNSIISNGYAV
                     MYIIAQKLVAKSLSDGYLVGSRGSVGSSFAATMSDITEVNPLPAHYICENEDCKYSFF
                     YEIGEWGSGVDLPDKDCPKCGRKLAKNGHDIPFEVFLGFEGDKEPDIDLNFSGNYQPT
                     IHKYTEELFGEGYVYRAGTIGTVAEKTAFGYARKYVEENNISVPNAEVLRLSNGCTGV
                     KRTSGQHPGGVMVIPDYKDVYDFTPIQYPANDTSCGVITTHFDYHSISGRILKLDILG
                     HDGPTIIRMLEDMTGINITEIPLDDKETMSLFTSTEALGVTPEEINCPIGCLAVPEFG
                     TKFVRQMLLDTKPTTFAELVRISGLSHGTDVWVNNAQDLVREDIVGLKDVISTRDDIM
                     NYLIFKGLPPKMSFTIMESVRKGKGLKPEHIEEMKKNNVPEWYIGSCKKIKYMFPKAH
                     AVAYVMTSFRIAYCKVHYPEAFYATYFTTKVEDFDADLIVKGLDAIKSKINEIESLGN
                     DATTKEKGMLTVLEVALEMYARGIKLLPVDIYKSDATEFIVVGEKTLLPPMAAIQGLG
                     GNAAINIQNERKNGEFISKEDLRKRTKISKTVIETLTNHGSLENMSERNQLSLF"
     gene            8378..8839
                     /locus_tag="PC471_001434"
     CDS             8378..8839
                     /locus_tag="PC471_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HDF2788569.1"
                     /translation="MKKNLEATIEEIVTKITDEHGFEMVDVEYVKEAGEYYLRVYIDK
                     EEGISLNECELVSRELSPILDEKDPIKENYFLEVSSPGLDRALKKDRDFVRYQGRDVD
                     LKLYKPLNGCKQFEGELVGLTEDNNIKIIANGKEMEFNRKDVAIVRLAIKF"
     gene            8869..10023
                     /gene="nusA"
                     /locus_tag="PC471_001435"
     CDS             8869..10023
                     /gene="nusA"
                     /locus_tag="PC471_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HDF2788570.1"
                     /translation="MNHEFMEALDELVVDRGIDKEILIDTIEQALLTAYKKNFGSAQN
                     VRVEFDRDKGDIKVFSQRVVVDESDLYDTFLEIELADAREINPNYELGDIIEHEVTPK
                     NFGRIAAQTAKQVVVQRIREAEREIVYNEFIEKENEIVTGEVARANKNVVYVNLGRIE
                     GIMTQSEQIPFENYKAGQKIKFYILEVKKTNKGPQIVVSRSHPGLVKRLFEEEVPEIY
                     EGIVQIKSVAREAGSRTKMAVRSIDEKIDPIGACVGPKGLRVKNIVDELGDEKIDIIK
                     YSEDPAEFISAALSPSKVVRVDIDEDEKSALVIVPDYQLSLAIGKEGQNARLAAKLTN
                     WKIDIKSESQAEQEMLNLENSKEIEAEDVEAEFIDQEELDTSEDNFSEEE"
     gene            10030..10311
                     /locus_tag="PC471_001436"
     CDS             10030..10311
                     /locus_tag="PC471_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HDF2788571.1"
                     /translation="MILKKVKKVPQRKCIACQDRDSKKELIRIVKNKEGKIFLDPTGR
                     ANGRGAYVCKSSECLKKAIKSKALNKAFKIDVPDEVYDNLLMELEEYEK"
     gene            10301..10612
                     /locus_tag="PC471_001437"
     CDS             10301..10612
                     /locus_tag="PC471_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal L7Ae/L30e/S12e/Gadd45 family protein"
                     /protein_id="HDF2788572.1"
                     /translation="MKNNKEKVLSFLGLATRAGKIVSGDDSTLLDLKKGKVNLILIAE
                     DASNNTKKLFKDKSTFRNIPYLFFSTKEEIGFAIGKSPRAVVGIKDENFSKKIIELIE
                     I"
     gene            10634..12574
                     /gene="infB"
                     /locus_tag="PC471_001438"
     CDS             10634..12574
                     /gene="infB"
                     /locus_tag="PC471_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HDF2788573.1"
                     /translation="MSKTRVYQIAEELNISNEELINKLAELDINVTDKDSVLEGEELE
                     LALEMLGEDLSQENGNVIEIDGKLTVQVLATKLDKSPSEIIMKLMKMGTMATINQEIS
                     FEIAALAAKDYGFELTVAESDDTEALEIEALMEIEEDKEEDLKPRPPVVTVMGHVDHG
                     KTSLLDAIRKTDVISGEAGGITQHIGASEVKINGHKIVFLDTPGHEAFTSMRARGAQV
                     TDIAILVVAADDGIMPQTVEAINHAKAAGVPLIVAINKIDKPGANPDKVKQELADQGL
                     LVEDWGGEVIAVPVSAKKKEGIDTLLEMVLLVAEMEELRANPNKRAVGTVIEAELDKG
                     RGPVATVLVQGGTLTVGDPIVAGVACGKVRAMINAKGKRVKTAGPSTAVEILGLSEVP
                     QGGDQFVEVPTDKIARSVAARRQQIVRDEMLKSTQRLSLDALFSQMSEGSIKDLNIVI
                     KADVQGSVQAVKQSLEKLSNEEVQVKVIHGGVGAVTESDILLAAASNAIIIGFNVRPV
                     PGAESLGEKENVDIRTYTIIYKAIEDIQAAMTGMLDPEYVDEETGKAEIREIYKISGV
                     GTVAGCYVTNGKIFRNCKVRLVRDSIIIHEGELAALKRFKDDVKEVNSGYECGMSFVN
                     YNDIKEGDIVEAYITKEVERKL"
     gene            12617..12997
                     /gene="rbfA"
                     /locus_tag="PC471_001439"
     CDS             12617..12997
                     /gene="rbfA"
                     /locus_tag="PC471_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HDF2788574.1"
                     /translation="MASYNRTRRIAEEIRKVVSTMLINGVKDPRITSMVSVTDVEVTN
                     DLRYAYVYVSILGGDEESTLTGLKSAGGYIRREVGKNIKLRYIPEIVFKLDDSIEKGM
                     YMDSLIKRVNEKNAQQNEDENYDE"
     gene            12978..13961
                     /locus_tag="PC471_001440"
     CDS             12978..13961
                     /locus_tag="PC471_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HDF2788575.1"
                     /translation="MKIMMNNMDSIINYIYEGDYFIVTSHISPDGDNIGSTLSMYYTL
                     KNLGKNVYYVLDDEAPLNLRFLVEDVKIYKSNEFNMKNYSLIALDCGDKMRICVSDEI
                     KNNASKLICIDHHASNDSYGDLNYVDIDASSTCELVYNLLVRFQQIKDINIINEDIAT
                     CLYTGLVTDTGNFSYSNVHASSFEMAKNLLVLGAQKNTIIQNIYQSNSSGYYKLLGEA
                     LKGLEIFDSKVSSIVLTQDMMNRNNISFNDVDGITTYTRDIKGIEVGILFKEKKQNEI
                     KVSFRSKNYVDVSEIAKLFGGGGHVRAAGCTIRDSIDNAKKMVLEAVLKSI"
     gene            13974..14879
                     /gene="truB"
                     /locus_tag="PC471_001441"
     CDS             13974..14879
                     /gene="truB"
                     /locus_tag="PC471_001441"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HDF2788576.1"
                     /translation="MNKIISILKPTGMTSHDVVSRVRKILNIKKVGHTGTLDPDASGV
                     LPICIGKATKVCEVILNKDKSYICELTLGISTDTYDASGEILKKVDDFKFSNEDIERA
                     FDTQRGEINQLPPIYSALKVNGKRMCDLVRSGRQSEITLKTRRVNIKDIKILSIKGNK
                     VMFYVECSKGTYVRSICHDIGEYLGCGAHMSFLNRTSSGKFDLDNSITLEELELFYEN
                     KTLDKYLYDIDYVLDSFNYVVLNPNAIKYYSNGGSIDDKRFLKNNFDKDDEFVRVYST
                     DNFLGLGKLSKHNNTISVKSDKMFI"
     gene            15010..15939
                     /locus_tag="PC471_001442"
     CDS             15010..15939
                     /locus_tag="PC471_001442"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HDF2788577.1"
                     /translation="MVIIKSIKEIANIKESVITIGNFDGVHKGHQVLIGKTVEHAKKE
                     NIQSIVFTFANHPVNYFRPNSTKNIISNDDKIKLLEDLGIDIVVIIPFDEYMTKIPAK
                     DFVDEILVKKLKAKKIVIGHDFTFARAKEGNVNLLKSLEHEFGFEVEVIKPIKINDVR
                     VSSTYIRSLVSQGDMANVKEYLGRNYKLEGCVIHSKHLGRTIGFPTANIDLKNNMLVP
                     KRGIYASIVHIGDETYFGATNVGYNPTVNGKTLSIETNILEFDRDIYGENIIVEFLER
                     IRDERKFNSIDDLKNQLYNDTNYVYENYVCKNK"
     gene            16043..16300
                     /gene="rpsO"
                     /locus_tag="PC471_001443"
     CDS             16043..16300
                     /gene="rpsO"
                     /locus_tag="PC471_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002843739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HDF2788578.1"
                     /translation="MINKSEIIKEYGRAEGDTGSPEVQIALLTARINELNGHLKTHKK
                     DHHSRRGLLKMVGRRRNLLAYLKEKDLEGYRALIAKLGLRK"
     gene            16540..17385
                     /locus_tag="PC471_001444"
     CDS             16540..17385
                     /locus_tag="PC471_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HDF2788579.1"
                     /translation="MKIKLVCDSLCDIPDEISEKDYIEIVPLTVIFNDREYIEGVDIK
                     KEEFYKKVKEIKQIPKTSQATYMEFKEVFDKFITEGYHIICMTGAANASGTFQSAMIA
                     KNDVNENEKIHIFDTRNLSLGSGQYVIKACELLEEGLGFEEIIDELENTRSSVRLLFA
                     PYTLDFLKQSGRVPVATALIGNMLNIKPIFFFDNGEAKLVNKVRGIKNIASKLVDIIL
                     EMNEGSLEGKIVTIGCGDNLHDCEILKDEVNKRIKARRVLFTRGGVSICSHTGPDILA
                     ISCSN"
     gene            17510..19621
                     /gene="pnp"
                     /locus_tag="PC471_001445"
     CDS             17510..19621
                     /gene="pnp"
                     /locus_tag="PC471_001445"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HDF2788580.1"
                     /translation="MFEHKIFKMDFAGRELSVEIGKICEMASGSCIVRYSDSMVMVNT
                     TKSAKPRDGIDFFPLSVDYEEKLYSVGKIPGGFLKREGKPSEKAILTSRLIDRPIRPL
                     FPKGFRNDVQVVATVLSVDQDCTPDIVAMIGSSIALSISDIPFNGPTGSVCVGLVDGA
                     FVVNPNAEQREKSSMHLVVSGTKEAIMMVEAGADEVPDEVMLDAILFAHQEIKKIVEF
                     IEGIVAEVGKEKMPVELYHAGEEITQLVREFATDKMKKAVQTFEKLERMENMDRVKEE
                     TLAHFEETLEDFEDFVGDIEEVLQDIIKEEVRKLIVHENVRPDNRKLEEIRPIWCETG
                     MIPRAHGSAIFTRGQTQVLNVATLGALGDVQKLDGLDEEENKRYMHHYNFPAYSVGEA
                     RPSRGPGRREIGHGALAERALLPVIPSQEEFPYAIRLVSEVLSSNGSTSQASVCGSTL
                     SLLDAGVPIKDMVAGIAMGLIKHDGKVAVLSDIQGMEDHLGDMDFKVAGTEYGITAIQ
                     MDIKIDGIDKEILQRALKQAKEGRIHILGEMRKTISQPKPELSPYAPKIVKMQINPDK
                     IKDVIGPGGKIITKIIDETGVKIDIEQTGEVFISGIEIDMIKKAQELINNIVVEPEVG
                     KTYKGKVSRIMNFGAFVEILPGKEGLLHISHIAHERVAKVEDVLNIGDEVEVKVTEID
                     EKGRVNLSRKVLLPKPEHKNK"
     gene            19887..20630
                     /locus_tag="PC471_001446"
     CDS             19887..20630
                     /locus_tag="PC471_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HDF2788581.1"
                     /translation="MIMMVLNKSKLKKIIILILVVLLVLSGGVMVLKLFNNKPVFNLF
                     NGVDLPYERGTKDKSGYVALTCNIDLGWETEYVESILETLKKENVKITFNVTGKWAEK
                     NKDELLKIKKQGHEIGNHGYKHLDYSTLSYEDNYEQIETSKKIIEEIIGEKTKFFQAP
                     AGSFGPETVKAAKALGYTSIKWDADTIDWKYKDQPEVIIDRMKKKDIKDSSIILMHPT
                     NATTKCIDDIIAIVREKGLKAGKLSDVFK"
     gene            20788..22035
                     /locus_tag="PC471_001447"
     CDS             20788..22035
                     /locus_tag="PC471_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HDF2788582.1"
                     /translation="MYKTKILENGLTIIGEEIPYLKSITLGIWINAGSRIEEAQVSGT
                     SHFIEHMMFKGTKNRTSKEIASSIDNLGGQINAFTSKECTCYYVKLIDEHIDTGIDVL
                     SDMILNSKFDKNDIDKERLIILEELKMYEDSPDDLSYDLLVENIYANDGLGMNIIGTK
                     ESLYNITRESMLEYLNKYYIPNNAVISIAGNFNFDDMVEKIKSKFGHWEKKNLSIDIS
                     EAKFNPCFISKNKDTEQVNLAMCLKGIPFENDEEVYSMAVVNNIFGGSISSRLFQKIR
                     EEKGLVYSIYSSQTLYRKCGELGIFASMSTENLQDVYNLIKKEIENIRENYLTEKEIS
                     ESKEQLKGNYILDLESTSSRMMSTGKSMLLSKKVKTTDEILECINSVNINSIKKVVDK
                     VFNIENIGTCIVGRDVEKILHLD"
     gene            22070..22315
                     /locus_tag="PC471_001448"
     CDS             22070..22315
                     /locus_tag="PC471_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlmC/YmxH family sporulation protein"
                     /protein_id="HDF2788583.1"
                     /translation="MNLSEIAGKEIVNLVTGERLGVIGECDLILDETTGGILALLIPK
                     EKGFFARRKDKSFLEVPWRNVKKIGNDMIIIEYEGYI"
     gene            22325..23548
                     /gene="dapG"
                     /locus_tag="PC471_001449"
     CDS             22325..23548
                     /gene="dapG"
                     /locus_tag="PC471_001449"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HDF2788584.1"
                     /translation="MSILVQKFGGTSVESYEKMNEVCKIVKAYKKNDEELQLVLVVSA
                     MGRKGAPYATDTLINLCSAVNDEPSKRELDLIMSCGEIISGTILANLLNAQGIDSVFF
                     TGQQAGIITSDEYSNAKIKYINPKKVKRALDDGKVVVIAGFQGVTDDGEITTLGRGGS
                     DTSAVAIGKALECETVEIYTDVDGIMTADPRVEPNAKVLSFIDYEEVFQMADKGAKVI
                     HPRAVQLAKSGNITLAIKNTMNPTFEGTKIGSLCRHLEDNIEYEQERDFKVAVANKDS
                     VAQVKIKSAEEVFTEVLNEIEKKLITIDMINFFISEKAFVVEDADIKSLKEILDKFEL
                     DYEVNRDCAKVTLICSRIADEMSGIMSKVVRGLSKAGVSLLQTSDSNMTISCLVSEED
                     MHTAVHAIHQQFYLK"
     gene            23661..24395
                     /locus_tag="PC471_001450"
     CDS             23661..24395
                     /locus_tag="PC471_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HDF2788585.1"
                     /translation="MKDYREDNERFDKDNCSEESSCEERECIKQFGTNEMPPQPPKDI
                     QCITIIGEIEGHFIGNPQKKATKYEHIIPMLYSIEESNDVKGVLVVLNTVGGDIEAGL
                     AIAELLNSTSKKVVTLVLGGSHSIGVPLATAGDYSFIAPTATMIIHPVRTTGLVIGIN
                     ETFEYFKKMQDRIIQFIIRTSNIKKDVLEKLMHEKDELVSDVGSVLIGKEAVDYGLID
                     EVGGLKEALKKLRELIKESEEEKNND"
     gene            24518..26929
                     /locus_tag="PC471_001451"
     CDS             24518..26929
                     /locus_tag="PC471_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HDF2788586.1"
                     /translation="MATKKKKKKKVNTLSFNAEYHNLITIFIGVFLLYSLNSNSMGWI
                     PVLMQNLFKGLFGGLSIAIPFIVIITGLLGFFDGNEYIYRLRKTKLYYIIILFIFVFY
                     GLLNAGTLPVDSPLKGNMFDDVMKLGVSGQGSGLIATTIAYYMSKIFGIAGGWLISIF
                     ALILSVMFIFNISIKDLVSNAKSKSKASKDSNLTFKDKIANMKKSAIDMMTDEVDDTT
                     INKKPGFFKGLMSKGLNNDDEEDEYFEEENSDGVDDKTIKIVGFNKAEDEYLEILEGT
                     QSMPELDVLKELQKATNENPVVDTKPEKKVDIAKPNLNIEKTQPMSIVAEPVNEDYSN
                     YKKPSIELLNKVNKKSDENGKKKVLKNASLLEKTLSDFGVEAKINQVTVGPTITRYEI
                     QPSPGVKVSKIVNLTDDIALSLAAKSIRIEAPIPGKSAIGIEVPNEEAQMVGVREVLE
                     SEEFNNFNSPLAMGLGKDVAGKIIIGDIGKMPHLLIAGSTGSGKSVCVNTLISSILYK
                     ANPDEVKLLLIDPKVVELANYNGIPHLLIPVVTDPKKAANALNWAVTEMNRRYKLFAD
                     AQVKDVTSYNEKAEEKLPKIVIIIDELADLMMASANDVEDYICRLAQMARAAGMHLIV
                     ATQRPSVDVITGVIKANIPSRIAFAVSSQTDSRTILDMGGAEKLLGKGDMLFYPLGAA
                     KPVRLQGAFISESESEKVIDFVKSQVKDGIKYEEDIIETISKVNTSKGSDEDEFLSEA
                     IEFVVESGQASASMLQRRFKIGFNRAARLIDSMEERGIIGASEGSKPRKVLISKQDLQ
                     NLEGE"
     gene            26932..27990
                     /gene="mnmH"
                     /locus_tag="PC471_001452"
     CDS             26932..27990
                     /gene="mnmH"
                     /locus_tag="PC471_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HDF2788587.1"
                     /translation="MSVIKIEKALKLDKVIFVDVRTEGEYEEDHILNAINMPLFKNNE
                     HNDVGTIYKMQGKHEAIQKGFDYVSYKLKDIYLQAAELALNYDNIVIYCARGGMRSGS
                     IVNLLSSLGVNVYQLEGGYKAYRNFVLEYLRHVMDTKNFVSVHGLTGTGKTDLLHLLD
                     EKGIDVLDLEGIAKNSGSTFGFITFDKKPPSQKNFETKLFESIFFSKENYIFVESESK
                     RVGSVTVPNEIFEAMTREGHHILLECDIENRVDRLCRDYIYDKDEGNILILKECINKF
                     RKRLGNKVVDDYIDLLESGEYKELVKRYLLEYYDPLYMHSVEKYKYSKIINFNDTQKA
                     LEDVIDLYESILEGESEC"
     gene            27984..29318
                     /gene="rimO"
                     /locus_tag="PC471_001453"
     CDS             27984..29318
                     /gene="rimO"
                     /locus_tag="PC471_001453"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HDF2788588.1"
                     /translation="MLKIALESLGCSKNLVDAEIMMGILNNKGYKLIGDFEEADVIIV
                     NTCGFIESAKQESIDTIINFAELKKTGNLKLLIVTGCLAQRYSEELKTEIPEIDAIVG
                     TGSYQNIDKILKELSEMHKIVSLNDIEFVFNEDLPRYISTPSYMAYLKIGEGCSNNCT
                     YCIIPKLRGKYRSRKFEDIIKEAKKLAESGVKELVVIAQDTTKYGFDLYGKERLSELL
                     EELAKIDGFKWIRVMYSYPESITEELIQVIKKYDNICSYFDMPIQHASNNILKLMNRK
                     TTKEDILNKINLIRSNIPDAILRTTIIVGFPGETEDDFKQLVDFVEEVKFDRLGAFAY
                     SREEDTPADRLPNHIDEEVKIQRRDTLMMIQQKISEELNDKKIGKTYEVLIEEQIEDN
                     VYTGRTQGDAEEIDSIVYVKSVDNLEVGEFVSVQINDAMEYDLMGDVLYELA"
     gene            29305..29847
                     /gene="pgsA"
                     /locus_tag="PC471_001454"
     CDS             29305..29847
                     /gene="pgsA"
                     /locus_tag="PC471_001454"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HDF2788589.1"
                     /translation="MNLPNKLTLFRIFLIPVFVLIMLLNIPNKFLIACIIFIIASITD
                     ALDGKIARKYNLVTDFGKFMDPLADKLLVISALTCMIEDHLVSSWMVIIIVARELTVS
                     ILRAIAAADGKVIAAGNSGKLKTITQMVSIVFLLLGAQFENVLILNIGEILILIATLL
                     TLYSGWEYLYKNKELFMSSK"
     gene            30135..31181
                     /gene="recA"
                     /locus_tag="PC471_001455"
     CDS             30135..31181
                     /gene="recA"
                     /locus_tag="PC471_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HDF2788590.1"
                     /translation="MSVDQEKLKALNEALGKIEKDFGKGSVMKLGEATSMSIDVISTG
                     AIGLDIAIGIGGLPRGRIVEVYGPESSGKTTVALSCVASAQKDGGIAAFIDAEHALDP
                     VYAKALGVDVDNLIISQPDTGEQALEIAEALIRSGAIDIIVIDSVAALVPKAEIDGDM
                     GDSHVGLQARLMSQALRKLTGSIKKSNCVAIFINQLREKVGIMFGNPETTTGGRALKF
                     YSSVRLDVRKIDTIKQGDKVIGSRTRVKVVKNKVAPPFKQAEFDIMYGEGISKIGDLL
                     DIAADVDIVKKSGSWYSYNDTKLGQGRENVKKFLEDNLDLTTEIDEKVRAFYNLNEEH
                     EESGTSVSKEIVEE"
     gene            31357..32898
                     /gene="rny"
                     /locus_tag="PC471_001456"
     CDS             31357..32898
                     /gene="rny"
                     /locus_tag="PC471_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HDF2788591.1"
                     /translation="MDVIDSVVMIVIGAAVGIIAGYFVRKNISEAKIGQAENLAKEII
                     DKAHHDSETVQKEKLLEAKEEIHKWRTEAERENRERRTEVQKYEKRVVQKEEVLDRKL
                     QNLESKEVNLSEKLKVVEKKEEEVEVIKTQQLEKLESISGITSDKAKEIILTNAERDV
                     RREMSIMIKEIESQAKEEADKKSREIIGYAIQKCAADHVAETTVTVVSLPNDEMKGRI
                     IGREGRNIRTLETLTGIDLIIDDTPEAVILSGFDPIRREIARIALEKLIADGRIHPAR
                     IEEMVEKARKEVDNIIKEYGEQAAFETSVHGIHPELIRLLGRLNYRTSYGQNVLKHSI
                     EVAHIAGIMAAEIGADIRLAKRAGLLHDIGKAVDHEMEGTHVEIGMDLLRRYKESKEV
                     IHAMSTHHGDYEPQTVEAVLVTAADAISAARPGARRETLEAYIKRLEKLEEIANSYEG
                     VEKSFAIQAGREIRIMVKPEAINDEEIHLLARDMTKKIEDELEYPGQIKVSIIRETRA
                     IEYAK"
     gene            33212..35548
                     /locus_tag="PC471_001457"
     CDS             33212..35548
                     /locus_tag="PC471_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HDF2788592.1"
                     /translation="MKKNTLLHTNIMVCIIITLGFVATSIIGYQSNTNIFKNDIEHVS
                     TLAAEGIYYQIDKLLSEPINVSLTMANDSLLKNFLDGEKEHLNDEEFIYKLQDYLNVY
                     RNKYSYDSVFLVSTKTNNYYHFNGLDRNLSANNSENQWYYTFLKNDDEYSLNVDNDEA
                     SNNSITVFVNCKIKDDNGATMGIIGVGLKVNSLQMLLKGYNDKFDVVARLIDDKGFVQ
                     LAVDKTGHENINFFENSSSDLSNSKQLILNNKKEQKSFWYSSEKSKSYIVCQYIPSLK
                     WHLVLENDFTLMIKQLHLQFFKGIAVIVLIIIFVLFTITHVLKKYKNQIIKLSVSQKL
                     EYQKLLSKSTEGLYENIFEIDITNNKAGCEDENRYFHEFGLGLNTPYDEALHTIAKNQ
                     IKEEYAQGYLDTFLPENVIKRYNSGINNISYDFLIKKDEESYHWIRINARIFFWTSDE
                     SVRMISYRKNIDEEKNRELLLLERSQRDSLTGLYNKATTEEFIRSFLKNESNQDLEHV
                     FLIFDIDNFKNVNDKYGHVFGDFVISEFALELKSQFREDDIVGRIGGDEFVVLIKDFN
                     NNNYVLIDKLERFCSKINQKKFVEQAEFSIACSIGVAMYPEHGYVYSELYEKADQALY
                     YAKSHGKNSYHIFSEDSIDSISFHIDSRDIKVLLEMATDGVVKFACMENLKLLYFNQK
                     MLEFTGYSAQTVTSIGFDIFSPVLQDDLQMVLDTLREAISKRETFEIVFRFCHTDGHI
                     FPVRMQGKFVSELYQNRYPVFYAIYTDISSINTLLENK"
BASE COUNT        14365 a         3797 c         6733 g        10724 t
ORIGIN      
        1 tagatatatc ctaattatgt caaaaaataa aaaaattttt atttattaga gtttttggaa
       61 ataataataa cagatattat aattaattaa cagtttttgt aagtatttga caatagagag
      121 ggtgtaacaa atgggaataa tagagtcagc atcaaaattg gctgagatgg ttcatttatt
      181 ggctgttgaa aaggggataa ctgatataga agcatgggat gaagctgtga aagaatacag
      241 caaaatttat gaagaaagaa gaaatgaata gaatgataaa aaatgaaaac ttgatgataa
      301 aaaataactt tatttaaaga aattcaattt ttatagaagt attaaaaata atataaataa
      361 aaagatggta tttcaaatgt aagtaaaaaa tcattttggg ataccatcct tattatttat
      421 tttaataata tataagtaat aaatcttact tatccatatt ttttatgtaa cctctttttt
      481 ctaacttttt tatatgctcc ttacgtattt catcaagatt aataccatat tgttcttcta
      541 gcttaaacat catagtaaca catgtctgag ctacatccat tagttcctta gcagttttct
      601 ttataatatc aacttcatct aaatcattat tttctccact tagatttctg aatttaccta
      661 tacattcagc taattcacct tgttcttcac ccattttgat aaatgtagat tctagtgttg
      721 ggtctaaatt attaagctct ggtaaagata tagtttttag tttcataaaa gctcctttca
      781 agttattcgt atttatacta aaacatcata acaaaaataa tactaaaaaa aaactttaaa
      841 ttcaacataa aaacttacat atataataaa aatcgtgaaa taaatctata tttgtcttaa
      901 tacagcctca tataaaactt ctccaatagg tttatttatt acaagagaag ctgacttatc
      961 atagactgta tttgctttat taataagagc aatataatca cctttgtaat aatctataaa
     1021 acttgctgct gggtacacag ctaatgatgt acctcctata atcaataaat cagcattact
     1081 tatagcagaa attgttttag taatgacatc actgtcaaga gcttcctcat aaagtactac
     1141 atctggtttt actatagaac cacagttgtc acagtaagga atattacctc ccagatttaa
     1201 catagattct aagtcaaaaa acttaccaca tttagtacaa taatttctat gtacagaacc
     1261 atgaagttct agcacatttt tacttccagc catttgatga agtccatcaa tattttgagt
     1321 tatgacagct ttaagcttac ccatttcttc gagttttgct aaagctatat gagcattatt
     1381 gggttttgca ttaggatata ttaatttatc cttataaaat tcaaaaaatt cttctggata
     1441 tctaacaaaa aaagtatgtg aaactaattg ttcagcagta aattgtttat tgagtttttg
     1501 gctaaataat ccagtagaac ttctaaagtc tggaatatta gattcagttg aaacacctgc
     1561 tccaccaaaa aatacaatat tattgtgatt agctattaag tcttttaagc tatttgcatc
     1621 catagttaca cctccaaatt attatatata aattgtacta catattaggt cgtttatgtt
     1681 ttattaattt aaataaatat atagtttaat aagttaaata aagggaatat aaataaaatg
     1741 tatacagagt ataaatagac aatatttact aaaataacaa gttgtcgtat aattatatta
     1801 aaattgtttt agtgtataag aagggcgggg gaagtacgtg aagaaaaaag cagctttagc
     1861 aacattggct atgttaccct taggtgtggt aaatgcacat gctgatggag atataggtat
     1921 agtgactata aattatttaa atgtaagaaa tgaaccaact gccgaaagta gcatagcctt
     1981 tgttgctaaa aaagatgata aagttttgat taaagactct tctaatggat ggtataaaat
     2041 aaaagctgaa tctggacaag aaggttgggc ttcatcaaaa tatatagcaa aatcaaatag
     2101 tgactcttta agaacgtcta caaataaaga aaaacaagtt atttcaaata gtttaaatat
     2161 gagaaatgga gcaggtacaa gttacagagt aataactgta ctaaaaaaag gtcaaaaagt
     2221 agaagtaata tcagagagta atggatggtc taaaatcaaa tatgatggaa gactaggata
     2281 tgtatctagc tcttatttag gagacgtttc aaactcaact aataaatcaa aaactaaaca
     2341 agtgaataca acttcactaa atgtaaggag tggaccaaat acaagttatg gtttgttagg
     2401 caagttgcca aaaggaagta aagtagaagt aatatcagaa ggtaatggat ggtccaaaat
     2461 aaagtataat ggaaaagatg cctatgtatc tagtatgtac ttatcggatg taagtcaaag
     2521 taattcagac aattctagtc aaagcaatga taaaaagaat actgataagg tcgtaaatac
     2581 agcttcttta aatgtaagaa gtggaccagg ttctacatat agtaagttgg gaaaagttta
     2641 taaaggaagt aaagtaactg tactatcaga aagtagtgga tgggctaaga ttaattttaa
     2701 caataaagaa gcatttgtag taggcaatta tttatctact tcagcagata cttcaaataa
     2761 taactcaaat agtaactctg ataacagttc taatagtaat ggcaataatt cttcatcatc
     2821 aggtcaagta aatggtatgt caggtataag tggggctaaa attgattata aatctttgag
     2881 ttatacttta gaatctcata taagtaaaca agttgagaaa gcagcatcag gaggaaatgt
     2941 gatagctcca agtaatagga aaagcactcc aagtcctgaa tttagtactt tttcagctca
     3001 aagaacaagc tcatttgtaa atgcaagttc aagtgatata gaatattatt taaatcctaa
     3061 aaattttaca aatactacta aaggtatgat gcagttttta aagattaaca gttatagaga
     3121 tggtatttca gaatcaagtc ttaactcgta tcttaatggt ttatcttcta gtgtatttaa
     3181 aaaccaggga gcagccttta taaatgctgc caagaagtat aatatagatg ttgtatatct
     3241 agtatctcat gctatgtggg aaactgctta tggtaagtct acacttgccc aaggtcaaac
     3301 attaacatct tataagggac aacctcttag taagccagtt aaagtataca atttttttgg
     3361 tataggagca atagataaaa gtgctaatgt ttcaggtgca gaagcagcgt attccaatgg
     3421 ctggactagt gtagaagcta caatagatgg ctctgcaaag tggatatcac aaaattacgt
     3481 aaatagttct aagtacaatc agaatactat ctacaagatg aagtggaatt atgattatac
     3541 atggcatcag tatgcaactg atgtgaactg ggctaatgga atttctggaa ttatggaaaa
     3601 tttaattggt ctttatggag gaggaagtag tttggttttt gaagttcctc aatacaagta
     3661 atacataaat aaaaaatata gactatgggt agttttcatt ttatttaaat tatctatagt
     3721 ctttttgtat ataatttata atctgtaaat ttaatgctaa caaaactttt atgttataat
     3781 attaattcta agtaaatata agtgagtaat aggtagaaat agataaaaag tggtaataat
     3841 atatataaag ataattttga gaaaggagga tatatatgga aagtattaag gaatatttgg
     3901 ataagttaga aattaataat agtggtcttg gaaagcaact taaagaagtt tatataaata
     3961 gagtagttta ttttaaggaa gataagatag tttattttta tctaacttct aaagatattg
     4021 tttctcatga acttttagat aaatttaaag aagaattgat gtataaactc gattatttta
     4081 aagatatgag gatgaagata agatttactg gtcttgaaag aaaatccaat aaagatgtta
     4141 taaaaaaata ttggaataat atattgtata tattaaagta tctttgtcca tctattgcag
     4201 ggtggtataa acaagtagaa tttctttgtt tagaagaaga attaaaaata aaacttccaa
     4261 aaggtatatt ttatgagcga ttaatgaaaa aaaatgtaac ttatgtactt aaaaccgtgc
     4321 ttagcgaaga gttgggatta gatttaaata taacaataga aaaagctgtt gatgaaaagg
     4381 taaataaaga aagactcata agaatcaatg atagagaaat ggaagaaaaa attagggctt
     4441 tagaaattgg aaaagttaat aactgtgaag aaaatgaaga ggaaagttat gtcattaagt
     4501 ctgatgtaga tgaaaattta atctatggag ataatgcaaa tgctatgatt gaaaatatag
     4561 ttgaattaaa tgcatcttct ggaacagttg cagttgttgg agatatattt gatgttgaca
     4621 ctaaagaact taaaaacgga aaaatactta tgatagcttc aattacagat tatactagct
     4681 caataagctg taaacttttt ttaacagata caaataagga tggagtactt gaaaatgtta
     4741 aaaaaggagc atacttaaaa atcaagggtg acatagttta tgatacatat caaagagaaa
     4801 ttagtatgat gattagtggg ataagaaaag aaactagaat agagagagaa gataaatcag
     4861 aggaaaaaag agtagaactc catgcacata cacaaatgtc ttctatggat gctatttgtt
     4921 ctgttaaaaa gcttgtagag cgtgcagcca aatggggtca ttctgctatt gctataacag
     4981 accatggtgt tgtacaagga tttccagatg ctatgaatgc aggtaagtca aataatataa
     5041 aaatacttta tggagtagaa gggtacttgg ttgaagatga ttctctcatt atagaggatg
     5101 caaatgataa agaactttcg cagacatttg ttgtatttga catagagaca actggttttt
     5161 ctaatacaaa tgataaaata actgagattg gtgctgtaaa aatagaaaac tttaagattg
     5221 tagatagatt tagtgagtta ataaatcctg aaaaagatat ttcttataag atacaggaat
     5281 tgacaggaat aactaatgaa ttaattaaag acaagccaac cattgaagaa gttcttccta
     5341 agtttatgga atttgttgga gatagtgtat tagtagcaca taatgcagag tttgatactg
     5401 gatttatatc gcaaaaatgt agggaacaag gcttgttata taataacaaa aaagtagata
     5461 cacttatgtt agcaagagta atgttgccaa acttaaaaag acatagatta aatgtcgttg
     5521 caaaagaact tggtattcct cttttaaatc accatagagc agtggatgat gctgaagcta
     5581 cagcacttat atttaataag tttttgcaga tgcttacaga aaaaggtgca aaaactctaa
     5641 gtgatgtaaa caatatactt ggaaagattg attacacaaa gcttagcaca aatcatataa
     5701 ccttgatagc aaaaaattct ataggtataa aaaatttata taaaataatt tcagatgcac
     5761 atgtaaatca tttttttaga gcaccaagaa tccttagaag tgtattaaat gaatataaag
     5821 aaggattaat aattggttcg gcttgtgaag ctggggtggt ttttcaagct gtaaagaaaa
     5881 acgtgtctga tgaggaaatg aaaaaaataa tagatttata cgattatatt gaagtaatgc
     5941 ctatagataa taatagattt atgattgata aaggagaagt aaaggacgaa gaagagttaa
     6001 gggagttgaa taggaaatta attgatacag ccaaaaaatt tgacaaaatt ccagtagcta
     6061 ctggggatgt tcatttttta gacaaacatg aagctgtctt aagaaaagtt cttaaatatt
     6121 ctcaaggttt taaagtagat gaagaagaaa cttatttaca ttttagaaca acagatgaaa
     6181 tgttggaaga gtttagttat cttggagaag aattggcata tgaagttgta gttgaaaata
     6241 gtaatctgat tgccgatatg gttgaagata taaaacctat accagatgat acttatcctc
     6301 caattataga gggctcagat gtggagttaa gagaaatgtg ttacaaaaaa gccaaacgaa
     6361 tctatggaga cccaatacca gaaatagttc aaaaaagact ggatagagaa ttaaattcaa
     6421 taataagcaa tgggtatgca gttatgtata ttatagctca aaaactggtt gcaaaatctc
     6481 tttcagatgg atatttagtt ggttcaagag gctcagttgg ctcatctttt gcagctacta
     6541 tgagtgatat aacagaagtt aatcctcttc cagcacatta tatttgtgaa aacgaagact
     6601 gtaaatattc attcttttat gaaataggtg aatggggctc aggtgttgat ttaccagaca
     6661 aggattgtcc aaagtgtgga agaaaacttg ctaaaaacgg tcatgatatt ccatttgagg
     6721 ttttcttagg ttttgaaggt gataaagaac cagatataga cttaaatttc tcaggaaatt
     6781 atcagccaac aattcataaa tacacagaag aactatttgg agaggggtat gtatataggg
     6841 ctgggactat aggtactgta gcagaaaaaa cagcttttgg atacgcaaga aaatatgtgg
     6901 aagaaaataa tataagtgta ccaaatgcag aagtacttag gctttcaaat ggatgtactg
     6961 gagtaaaaag aacctcagga caacatcctg gtggagttat ggttatacca gactataaag
     7021 atgtatacga ttttacacct atacagtatc cagcaaatga tacttcttgt ggtgttataa
     7081 ctactcactt tgattatcat tcaataagtg gtagaatact taaacttgat atacttggac
     7141 acgacggtcc taccattata aggatgcttg aagatatgac tggaataaat ataacagaaa
     7201 ttcctttaga tgataaagag actatgtctc tatttacatc tacagaagca ttaggtgtta
     7261 caccagaaga aataaactgt cctataggtt gccttgctgt accagagttt ggaactaagt
     7321 ttgtacgaca aatgttactt gacacaaaac ctactacatt tgcagaactt gtacgtatat
     7381 ctggtctctc tcatgggaca gatgtttggg taaataatgc acaagactta gttagagaag
     7441 atatagttgg attaaaagat gttatatcta cacgagatga cataatgaac tatttaatat
     7501 ttaaaggatt acctcccaaa atgagcttta caataatgga gagtgtaaga aaaggtaaag
     7561 gtttaaaacc agaacacatt gaagaaatga aaaaaaataa tgttccagaa tggtatatag
     7621 gctcatgtaa aaagattaag tacatgttcc caaaagcaca tgctgttgct tatgtaatga
     7681 catcatttag aattgcatat tgtaaagttc actatccaga agcattttat gcgacttatt
     7741 ttacgacaaa agttgaggat tttgatgcag atttaattgt taaaggatta gatgctataa
     7801 aatcaaaaat aaatgaaatt gagtcactgg gaaatgatgc aaccacaaaa gaaaaaggta
     7861 tgttgaccgt tttagaagtt gctcttgaaa tgtatgctag aggaataaaa ttattaccag
     7921 ttgatatata taaatcagat gcaactgagt ttatagttgt aggagaaaaa acattacttc
     7981 caccaatggc agcaatacaa ggtcttggag gaaatgcggc tataaacatc caaaatgaaa
     8041 gaaaaaatgg tgaatttata tcaaaagaag atttgagaaa gagaacaaaa atatcaaaaa
     8101 cggtaataga aactcttact aatcatggtt cacttgagaa tatgagtgag agaaatcaat
     8161 tgtcattgtt ttaagttagc ccaaaaagtt gcaaataaaa gaattttatg atatcatagt
     8221 atagaaaata gtatactttg cagaaactaa gagtgagaca gctgttctca ctcttttatt
     8281 ttaaatatat tattaaaaag ctggtttatt tttattaaat atattttatt taagattata
     8341 taaactggtc atttacaatt gaataggagg aactttaatg aaaaaaaatt tagaagctac
     8401 aattgaggag attgtaacta aaataacgga tgaacatgga tttgaaatgg ttgatgttga
     8461 atatgtaaaa gaagcaggag aatactactt aagagtatac atagataaag aagaaggaat
     8521 atctttaaat gaatgtgaat tagttagtag agaattaagt ccaatactag atgaaaaaga
     8581 ccctataaaa gaaaattact ttttagaggt atcttctcca gggcttgata gagcattaaa
     8641 aaaagacaga gattttgtta gatatcaagg tagagatgta gatttaaaat tatataaacc
     8701 attaaatgga tgtaagcaat ttgaaggtga acttgtagga ttaacagaag ataataatat
     8761 aaaaatcatt gcaaatggta aggaaatgga atttaataga aaagatgttg ctattgtcag
     8821 attagcaata aagttttagt ggtatattaa atagggagga tataaaaaat gaatcatgaa
     8881 tttatggaag cattagatga attagtagtt gacagaggta tagataaaga aatacttata
     8941 gatactatag aacaagctct tttaactgca tacaagaaaa actttggttc agcacaaaat
     9001 gttagagttg aatttgatag agacaaggga gatataaaag tattttctca aagagttgtt
     9061 gttgatgaat cagatttata tgatactttc ttagaaatag agttagcaga tgctagagag
     9121 ataaacccta attatgaatt aggtgatatt atagaacatg aagttacacc taaaaacttt
     9181 ggaagaattg cagctcaaac agcaaagcaa gtagttgttc aaagaataag ggaagctgag
     9241 agagaaatag tgtataatga atttatagaa aaagaaaatg aaatagttac aggagaggtt
     9301 gctagagcaa ataaaaatgt tgtttatgta aatcttggaa gaatagaagg tataatgact
     9361 caaagtgaac aaataccatt tgagaattat aaagcaggtc aaaaaataaa attctatata
     9421 cttgaagtta aaaagactaa caaaggacct caaatagttg tatcaagaag tcatccgggt
     9481 ctagtaaaga gattgtttga agaagaagtt cctgaaatat atgaaggaat agttcaaata
     9541 aaatctgtag caagagaagc agggtcaaga actaaaatgg cagtaagatc tatagatgaa
     9601 aaaatagacc caattggagc ttgtgttgga ccaaaaggac ttagagtaaa aaatatagtg
     9661 gatgaacttg gcgatgagaa aatagatata ataaaatata gtgaagaccc agcagagttt
     9721 atatcagcag cacttagccc atcaaaggtt gttagagtag atatagatga agatgaaaag
     9781 tctgccttag taatcgttcc agattatcag ttatcgcttg ctataggtaa agaaggtcaa
     9841 aatgcaagac tagcagcaaa attgactaac tggaaaatag atattaagag cgaatctcaa
     9901 gcagaacaag aaatgttaaa tcttgaaaat tccaaagaaa tagaagctga ggatgttgaa
     9961 gctgaattta tcgaccaaga agagttagat acttcagagg ataattttag tgaagaagag
    10021 taaggaggga tgattttgaa aaaagtaaaa aaagtgcctc aaagaaaatg catagcatgt
    10081 caagatagag actctaaaaa agagcttata agaatagtta aaaataaaga aggaaaaata
    10141 tttttagacc caacaggaag agccaatgga agaggagcat atgtatgtaa aagctctgaa
    10201 tgtctaaaaa aagctataaa gagtaaagca ttaaataaag cttttaaaat agatgttcct
    10261 gatgaagtct atgataatct acttatggag ttagaagaat atgaaaaata ataaagaaaa
    10321 agtactttcg ttcttagggc tcgcaactcg tgcagggaaa atagtgtctg gtgatgattc
    10381 aactttgctt gaccttaaaa aaggaaaagt aaatttgatt ttaatcgcag aagatgcatc
    10441 aaataatact aaaaaacttt ttaaagataa atcaactttt agaaatattc catatctatt
    10501 tttttcaaca aaagaggaaa taggatttgc aataggaaaa tctccaagag cagttgtagg
    10561 aataaaagat gaaaattttt ctaaaaagat aattgaacta atagaaattt aaaataaaat
    10621 agggggtgat cttgtgtcaa aaacaagagt ataccaaata gcagaagaat taaatatatc
    10681 aaatgaagag cttataaata agttagctga attagatata aatgtaactg acaaagattc
    10741 agtattagaa ggtgaagagt tagaattagc acttgaaatg ttaggtgaag atttatctca
    10801 agaaaatgga aatgttatag aaatagatgg aaagttgaca gtacaagtat tagcaacgaa
    10861 attagataaa tctccatcag aaataattat gaagcttatg aaaatgggaa ctatggcaac
    10921 aataaatcaa gagataagtt ttgagattgc tgctttggct gccaaagact atggatttga
    10981 attaacagta gctgaaagtg atgatacaga agctttagaa atagaagctt taatggaaat
    11041 tgaagaagat aaagaagagg atttaaaacc tagaccacca gttgttacag ttatgggaca
    11101 tgttgaccat ggtaagacat ctttactaga tgcaataaga aaaactgatg tgatttcagg
    11161 tgaagctgga ggaataactc agcatatagg tgcttcagaa gttaaaatca atggacacaa
    11221 aatagtattt ttagatactc caggacatga ggctttcaca tcaatgagag caagaggagc
    11281 acaagttaca gatatagcaa tattagtagt tgcagcagat gatggtataa tgcctcaaac
    11341 agttgaagct ataaaccatg ctaaagcagc aggtgtacct ttaatagttg ctattaataa
    11401 aatagataaa ccaggtgcaa acccagataa ggttaaacag gaattagcag accaaggatt
    11461 attagttgaa gactggggtg gagaagtaat agcagttcca gtatcagcta agaaaaaaga
    11521 aggaatagat acacttttag aaatggtatt attggttgct gaaatggaag agttaagagc
    11581 taatccaaat aaaagagcag taggaactgt tatagaagct gagcttgata aaggaagagg
    11641 gccagttgca acagtacttg ttcaaggtgg tactttaaca gtaggtgacc caatcgttgc
    11701 aggtgttgca tgtggtaaag ttagagcaat gataaatgca aaaggtaaga gagtaaaaac
    11761 agcaggacca tctacagcag tcgagatatt aggtctatca gaagttccac aaggaggaga
    11821 ccaatttgtc gaagttccaa ctgataagat tgctagaagt gtagcagcaa gaagacaaca
    11881 aatagttaga gatgaaatgt taaaatcaac tcaaagatta tcactagatg ctctatttag
    11941 tcaaatgagt gaaggttcta taaaagacct taatatagtt ataaaagcag atgtacaagg
    12001 ttcagtacag gcagttaaac aatctttaga aaaattatca aatgaagaag ttcaagttaa
    12061 agtaatacat ggtggtgttg gagctgtaac agaatcagac atattacttg cagcagcttc
    12121 taacgcaata ataataggat tcaatgtaag accagttcca ggtgcagaat ctctaggaga
    12181 aaaagaaaat gttgatataa gaacatatac tataatatac aaggcaatag aagatataca
    12241 agcggctatg actggtatgt tagaccctga atatgttgat gaagaaacag gaaaagctga
    12301 aataagagaa atatataaaa tatctggtgt tggaacagta gctggatgtt atgtgactaa
    12361 tggtaagata tttagaaatt gtaaagtaag attagttaga gatagcataa taatacatga
    12421 aggtgagctt gcagcactta agagatttaa agatgatgta aaagaagtta attctggata
    12481 cgaatgtggt atgagttttg taaattataa cgacataaaa gaaggcgata tagtagaagc
    12541 ttatataact aaagaagtag aaagaaaatt ataaattcgt atcaaagaaa atcagttagt
    12601 aaagaaggtc gatggtatgg catcatataa tagaacaaga aggatagcag aagaaataag
    12661 aaaagtcgta agcactatgc ttataaatgg agtaaaagac cctagaataa catctatggt
    12721 gagtgtaact gatgtagaag ttacaaatga cctaagatat gcttatgtat atgtaagcat
    12781 tctaggtgga gatgaagaat ccacactaac aggtcttaaa agtgctggag gatatataag
    12841 aagagaagta ggaaaaaata taaaacttag atatattcct gaaatagtat ttaaacttga
    12901 tgattcaata gaaaaaggaa tgtatatgga tagccttata aaaagagtta atgaaaaaaa
    12961 tgctcaacaa aatgaggatg aaaattatga tgaataatat ggatagtatt ataaactata
    13021 tttatgaggg tgactatttt atagttacct ctcatatcag tccagatgga gacaatattg
    13081 gttctacact ttcaatgtat tatacattga agaatttagg aaaaaatgta tattatgtac
    13141 ttgatgatga agctccatta aatttaaggt ttttggttga agatgtaaaa atctataagt
    13201 ctaatgaatt taatatgaag aactattctc taattgcact tgattgtggg gataagatga
    13261 gaatatgtgt gagtgatgaa atcaaaaata atgcatcaaa attaatctgt attgaccatc
    13321 atgctagtaa tgattcatat ggagatttaa attatgtaga tattgatgca tcttctacat
    13381 gtgaattagt ttataattta ttagttagat ttcagcaaat aaaagatatt aatattataa
    13441 atgaagatat agcaacatgt ttatatacag gtcttgtaac tgatactgga aacttttcgt
    13501 attcaaatgt acatgcaagt agctttgaaa tggcaaaaaa tttacttgtt ttaggtgcac
    13561 aaaaaaatac tataatacaa aatatatatc aaagcaattc ttctggttat tacaaattat
    13621 taggagaagc tctaaagggt ttggagatat ttgattcaaa ggtatctagt atagttttaa
    13681 ctcaagacat gatgaataga aataatatta gctttaatga tgttgatgga ataacaacat
    13741 atacaagaga cataaaaggt atagaagttg gaattttatt taaagagaaa aaacaaaatg
    13801 aaattaaagt gagttttagg tcaaaaaatt atgttgatgt aagtgaaata gccaagttat
    13861 ttggtggtgg tggtcatgta agggctgctg gatgtactat tagagatagt attgataatg
    13921 caaagaaaat ggtactagag gcagtgttaa aatcaattta aggtgacagt aaaatgaata
    13981 agattataag tatattgaaa cccacaggta tgacatctca tgatgttgta agtagagtga
    14041 gaaagatttt aaatataaaa aaagttggtc atacaggtac tcttgaccca gatgcttcag
    14101 gtgttttacc aatctgtata gggaaagcta caaaagtatg tgaagttatt ttaaacaaag
    14161 ataaatcgta tatatgtgag cttactttag gtataagtac agatacttat gatgcttcag
    14221 gtgaaatttt aaaaaaagtt gatgatttta aatttagtaa tgaagacata gaaagagctt
    14281 ttgacacaca aagaggagag ataaatcaac ttcctccaat ttattcagct ttaaaggtaa
    14341 atggaaagag aatgtgtgat ttagtaagaa gtggtagaca atctgaaatt actttaaaaa
    14401 caagaagagt aaatataaaa gacataaaaa tactaagtat aaaaggtaat aaagtaatgt
    14461 tttatgttga gtgttctaaa gggacttatg tgagaagtat ttgccatgat ataggagaat
    14521 atctaggttg tggagctcat atgtcgtttt tgaatagaac atcttctggt aaatttgact
    14581 tagataattc aataacatta gaagaattag aattatttta tgaaaataaa actttagaca
    14641 aatacttata tgatatagat tatgtccttg attcgtttaa ttatgttgta ttaaacccta
    14701 atgctataaa atattatagt aatggtggct caatagatga taaaaggttt ttaaagaata
    14761 atttcgataa agatgatgaa tttgtaagag tatacagtac agataatttc ttaggattag
    14821 gaaagttatc aaaacacaat aatacaataa gtgttaaaag tgataagatg ttcatataac
    14881 caatgtattt aaaaaaacaa caaaagagat agtactataa taacttctat agtctatctc
    14941 ttttacttat gctataatag tttgtgaata agttaagagt aaattatatt ttagcgggga
    15001 acatttatta tggtgattat aaaatctata aaagaaatag caaacataaa agaaagtgtc
    15061 attaccatag gaaattttga tggtgtacac aaaggacatc aagttcttat aggaaaaaca
    15121 gttgaacatg caaaaaaaga aaatatacag agtattgtat ttacatttgc aaatcatcct
    15181 gtaaattact ttagaccaaa ctcaacaaaa aatattataa gcaatgatga taaaataaaa
    15241 ttattagaag atttgggaat agatatagtt gtaatcatac catttgatga gtatatgact
    15301 aaaatacctg caaaggattt cgtagatgag atactagtaa aaaaattaaa agctaaaaag
    15361 atagtaattg gacatgactt tacatttgca agggcaaaag aaggcaatgt taatctatta
    15421 aaaagcttag aacatgaatt tggttttgag gtagaagtaa taaaacccat caaaatcaat
    15481 gatgtaagag tgagtagtac ttatattaga agtttagtat cccagggtga tatggctaat
    15541 gtcaaggaat atttgggtag aaattacaag ttagaaggtt gtgttataca ttctaaacac
    15601 cttggaagaa ctataggatt cccaacggca aatattgacc ttaagaataa tatgctcgtt
    15661 ccaaagagag gtatttatgc ttctattgta catattggag atgagactta ttttggtgct
    15721 acaaatgtag gctataaccc aacagttaat ggaaaaactt tgtctattga aacaaatatt
    15781 ttagagtttg atagagatat ttatggggaa aatataattg ttgaattttt ggaaagaata
    15841 agagatgaaa gaaagtttaa ttcaatagat gacttaaaaa atcaattgta taatgataca
    15901 aattatgtgt atgaaaacta tgtttgcaaa aacaagtaat gtatgataaa atgattaagg
    15961 aaaataacca ttactcggca tttgaatact ccaatcaatg cttagtaata ggcgtttcta
    16021 aaaaaatacg gaggtaatct aaatgataaa caaatcagaa ataataaaag aatatggaag
    16081 agctgaaggg gatactggtt ctccagaagt acaaatagct ttattaacag ctagaataaa
    16141 cgagttaaat ggacatttaa aaactcataa gaaagaccac cattcaagaa gaggactatt
    16201 aaaaatggtt ggtagaagaa gaaacttatt agcttactta aaggaaaaag atcttgaagg
    16261 atatagagca ttaatagcta aattaggatt aagaaagtaa tttcattctt tataaaaaag
    16321 caggtatagt gtatacctgc ttttttatag ttaattcatt ataaagtaaa atagttaata
    16381 ataaaagtag tttatatgtt ataaaaaatt ataaaatgaa taatattggg taagaatatt
    16441 aaaatagtat acaaatacat aatcaattta aaaggaaaga caggataaat atagaaattt
    16501 tataacagga taattagttg gaataaggag gatattattt tgaaaataaa gttagtatgc
    16561 gatagtttgt gcgatattcc agatgaaata tcggaaaaag attatataga gatagttcca
    16621 cttacagtta tttttaatga tagggaatat atagaaggag tagatataaa gaaagaagag
    16681 ttttataaaa aagtaaaaga aatcaagcaa atacctaaaa cttcacaagc tacatatatg
    16741 gagtttaaag aagtgtttga taagttcata acagaaggtt atcatataat ctgtatgaca
    16801 ggggctgcaa acgcatcagg aacttttcaa agtgctatga tagctaaaaa tgatgtaaat
    16861 gaaaatgaaa aaatacatat atttgatact agaaatttat ctctaggtag tggacaatat
    16921 gttataaagg catgtgaact tcttgaagaa ggactgggat ttgaagaaat tatagatgaa
    16981 ttagaaaata ctcgcagtag tgtaagattg ctttttgcac catatacact tgatttttta
    17041 aagcagagtg gaagagttcc agttgcaact gctttgatag gaaatatgtt aaatataaag
    17101 cctatatttt tctttgataa tggggaagct aagttggtaa ataaggttag aggaataaag
    17161 aatatagcaa gtaaattggt cgatataatt ttagaaatga atgaagggtc tttagaagga
    17221 aaaatcgtca caataggttg tggagataat ttacacgatt gtgaaatatt aaaagatgaa
    17281 gtaaataaaa gaataaaagc aagaagagta ttatttactc gaggaggagt ttctatttgc
    17341 tctcatacag gaccagatat attggctata agttgttcta attagtagta atataaggtt
    17401 tttgcatata tatgtgaaga taattttata ttgttaattt atagtatttt tgataatata
    17461 gtaaatagag tgatatacgt gttttttaat aagaggagga caagcattaa tgtttgaaca
    17521 taaaatattt aaaatggatt ttgcaggaag agaactttct gtagaaatag gaaagatttg
    17581 tgaaatggct agtggtagtt gtatagttcg atacagtgat agtatggtaa tggtaaatac
    17641 aactaaatca gccaaaccta gagacggaat agactttttt cctttaagtg tggattatga
    17701 agaaaaatta tactctgtag gtaagatacc tggaggtttt ttaaagagag aaggcaagcc
    17761 ttcagagaaa gctatactta catcaagatt gatagataga ccaataagac ctttattccc
    17821 aaaaggattt agaaatgatg ttcaggtagt tgcaacagtt ctatcagtag accaagattg
    17881 tacaccagat atagtagcaa tgataggttc ttcaatagcc ctttctatat cagacatacc
    17941 atttaatgga ccaacaggtt cagtatgtgt tggccttgta gatggtgcat ttgttgtaaa
    18001 tccaaacgca gagcaaagag aaaaaagttc aatgcatcta gttgtttcag gtacaaagga
    18061 agcaataatg atggttgaag ccggagctga tgaagttcca gatgaggtaa tgcttgatgc
    18121 tattttattt gctcaccaag aaataaaaaa aatagttgag tttatagaag gtatagttgc
    18181 agaagttgga aaagaaaaaa tgcctgttga attataccat gcaggagaag aaataactca
    18241 attggttcgc gaatttgcaa ctgataagat gaaaaaagca gttcagactt ttgaaaagtt
    18301 agagagaatg gaaaatatgg atagagttaa agaagaaact ttagctcact ttgaagaaac
    18361 tttagaagat tttgaagatt ttgttggaga tatagaagaa gtattacaag atataataaa
    18421 agaagaagta agaaaactta tagtacatga aaatgtaaga ccagataata gaaaacttga
    18481 agaaataaga cctatatggt gtgaaactgg catgatacct agagctcatg gttcagccat
    18541 atttactaga ggacaaactc aagtacttaa tgttgctact ttaggtgctt taggagatgt
    18601 tcaaaaacta gatgggttag atgaagaaga aaacaagaga tatatgcatc attataattt
    18661 ccctgcatat agtgttggag aagctagacc atcaagaggt ccaggaagaa gagaaatagg
    18721 acatggtgct ttagcagaaa gagctttatt accagtaata ccatctcaag aagaatttcc
    18781 ttatgctatt agattagttt ctgaagtatt aagttcaaat ggttctacat ctcaagcaag
    18841 tgtatgtggt tcaacattat ctttacttga tgcaggtgta cctataaaag atatggttgc
    18901 aggtatagca atgggactta taaagcatga tggtaaggtt gcagtgcttt cagatataca
    18961 aggtatggaa gaccatttag gcgatatgga ctttaaagta gctggaacag agtatggtat
    19021 aacagcaata caaatggata taaaaattga tggtatagat aaagaaattc tacaacgtgc
    19081 tttaaaacaa gctaaagagg gtagaatcca tatactagga gagatgagaa aaactatatc
    19141 tcagccaaaa ccagaacttt caccatatgc acctaagatt gttaaaatgc aaataaatcc
    19201 tgacaagata aaagatgtaa taggacctgg tggaaagata ataactaaga taatagacga
    19261 aactggagtt aagatagata ttgaacaaac tggggaagta tttatatcag gtattgagat
    19321 tgatatgatt aagaaagctc aagaacttat aaataatata gtagttgaac cagaagtagg
    19381 aaaaacttat aaaggtaagg taagtagaat aatgaacttt ggagcatttg ttgagatact
    19441 tccaggaaaa gaaggattac ttcatatatc tcatatagct catgaaagag ttgccaaagt
    19501 tgaagatgta ttgaatatag gggatgaagt tgaagttaaa gttacagaga ttgatgaaaa
    19561 aggaagagtt aatttatcaa gaaaagttct tttaccaaaa ccagaacata aaaataaata
    19621 acttattata taattaaaag caaagtctta ggattttgct tttttaaata caaaaaagta
    19681 gataatttta aaatgatagt tatggatggc ttatagttct ttttctgtca gtctttactc
    19741 ttataaagaa gctatttaaa agctgattaa tagaaagctt tattgatata tatagaatca
    19801 cttaagttat atttagtgca taaacagagc ttatttttaa tagtattaaa taagaaacaa
    19861 tttgtacata attaggggga gttttaatga taatgatggt acttaataag agtaagctca
    19921 aaaaaatcat aatactaatt ttagtagtgt tattagtatt atcaggaggg gtaatggtat
    19981 taaagttatt taacaacaag ccagtattta atttatttaa tggtgtagat ttaccatatg
    20041 aaagaggtac aaaggataaa agtggatatg ttgcattaac ctgtaatata gaccttggat
    20101 gggaaacaga gtacgtagaa tccattttag aaacacttaa aaaagaaaat gtaaagatta
    20161 catttaatgt aactggaaaa tgggcagaaa agaataagga tgaattatta aagataaaaa
    20221 aacaaggaca tgaaataggt aatcatggat ataaacattt agattattct acattatcat
    20281 atgaagacaa ttatgagcaa atagagacct ctaaaaagat aattgaagaa attataggtg
    20341 aaaaaactaa attttttcaa gcaccagcag gttcctttgg tccagaaaca gtaaaggctg
    20401 ctaaagcatt agggtatact tctatcaaat gggatgccga tactatagat tggaaataca
    20461 aagaccaacc agaggtaatc attgatagaa tgaagaaaaa agacattaaa gatagtagta
    20521 taatcttaat gcatccaacc aacgcaacga caaaatgtat agatgatatt atagctattg
    20581 taagagaaaa aggactaaaa gcaggaaaac ttagtgatgt atttaagtag acaaagtata
    20641 aatatttggt taagattgta taagttgtag ttgaattaat taaaagactg acttataatg
    20701 taatacatat aaaaaataaa tataattaaa acatagtgtt ttcaataatc caatatgaaa
    20761 caaagaataa attgttagga gaatgtaatg tataagacaa aaatactgga aaatgggctt
    20821 acaataattg gtgaagaaat accatatcta aagtcaataa cattaggtat ttggataaat
    20881 gcaggctcta ggatagaaga agcacaggtg agtggaactt ctcattttat agagcatatg
    20941 atgtttaaag gaaccaaaaa cagaacatca aaagagattg ctagttcaat agataatttg
    21001 ggtggtcaaa taaatgcatt tacaagtaaa gaatgtacat gttattatgt aaaactaata
    21061 gatgagcata tagatacagg aattgatgta ttgagtgata tgatactaaa ttctaaattt
    21121 gataaaaatg atatagataa ggaaagactt ataatattgg aagaacttaa gatgtatgaa
    21181 gactctccag atgacttatc ttatgattta ttagtggaaa atatatatgc taatgatgga
    21241 ttaggaatga atataattgg gacaaaagaa tctctataca atataactag agaatccatg
    21301 ttagaatact taaataagta ttatattcca aataacgctg ttatttctat agctggaaac
    21361 tttaactttg atgatatggt ggagaaaatc aaatcaaaat ttggacactg ggaaaagaaa
    21421 aatttgagta tagacataag tgaggctaag tttaatcctt gctttatatc aaaaaataaa
    21481 gatactgaac aggtgaactt agctatgtgc ctaaaaggta ttccttttga aaatgatgaa
    21541 gaagtctatt caatggcagt ggtaaataat attttcggtg gaagtataag ctcaaggctt
    21601 tttcagaaaa taagggaaga aaaaggttta gtatattcaa tatactcatc acagacatta
    21661 tataggaaat gtggtgaatt aggtatattt gcaagcatga gtacagaaaa tttacaagat
    21721 gtatataatt taataaagaa agaaattgaa aatataagag aaaactatct tacagaaaaa
    21781 gaaatatctg aaagcaagga acagttaaaa ggaaattata tattggattt agaaagtact
    21841 agcagtagaa tgatgtctac tggtaaatct atgttactta gtaaaaaagt aaaaactaca
    21901 gatgaaatac ttgaatgtat aaatagtgtt aatattaatt caataaagaa ggttgttgat
    21961 aaggtattca atatcgaaaa tattggtaca tgtatagttg gaagagatgt agaaaagatt
    22021 ctacacttgg attaatattg aaaaaagtaa atctctggag gtataaaata tgaatttatc
    22081 tgaaatagca ggtaaggaaa tagttaactt ggtcacagga gaaagattgg gggtcattgg
    22141 agaatgtgac cttatactag atgaaactac aggcggtata ttagcgcttc taattccaaa
    22201 agaaaaagga ttttttgcca gaagaaaaga taagtcattt ttagaagtac catggagaaa
    22261 tgtaaaaaaa ataggtaatg acatgataat tatagaatat gagggatata tttaggagga
    22321 aaaaatgagt attttagtgc aaaagtttgg tgggacatca gtagaatcat atgaaaaaat
    22381 gaatgaagta tgtaagatag taaaagcata caaaaaaaat gatgaagaat tacaattagt
    22441 attagtagtt tcagcaatgg gaagaaaagg agctccatat gctacagata cacttataaa
    22501 tttgtgtagt gcagtaaatg atgagccaag taaaagagaa cttgatttaa taatgtcttg
    22561 tggagaaata atatcaggaa caattcttgc taatttattg aatgcacagg ggatagattc
    22621 tgttttcttt actggtcagc aagcaggtat aattacaagt gatgaatatt ctaatgcaaa
    22681 gataaagtat ataaatccta aaaaggtaaa aagagcttta gatgatggta aggttgtagt
    22741 tatagctggg ttccagggtg taacagatga tggagaaata actactcttg gaagaggcgg
    22801 aagcgataca tcagcagttg ctattggtaa agctttagaa tgtgaaacag ttgaaattta
    22861 cactgatgtg gatggtataa tgactgcaga ccctagagta gaaccaaacg ctaaagtatt
    22921 gagttttata gactatgagg aagtattcca aatggctgac aaaggtgcaa aagtaataca
    22981 tccaagagca gtacagttgg ctaaaagtgg aaatataact ttagccataa agaatacaat
    23041 gaatccaact tttgaaggta caaaaatagg ttctttatgt agacacttag aagacaatat
    23101 agaatatgaa caagaaagag attttaaggt tgctgttgca aataaagata gcgtagcaca
    23161 agttaagatt aagtcagcag aagaagtgtt tacagaagta ttaaatgaga tagaaaagaa
    23221 acttataact atagatatga taaatttctt tatatcagaa aaagcatttg ttgtagaaga
    23281 tgctgatata aaatcattaa aggaaatact agataaattt gaattagatt atgaagtaaa
    23341 tagagactgt gctaaggtaa cattgatatg ttcaagaatt gctgatgaaa tgtctggtat
    23401 catgtcaaag gtagttagag gattatcaaa agcaggggta tcccttttac aaacatcaga
    23461 ctcaaatatg actatatctt gtttagttag tgaagaagat atgcatacag cagtacatgc
    23521 tatacatcaa caattttatt taaaataaac tgtataatac aattattttt taatcaaatt
    23581 taagaatttc atatatatct taatatattt gatttatttg gtaaaactaa attaaatata
    23641 attttgatag gagttttttt atgaaagatt atagagaaga taatgaaaga tttgataaag
    23701 ataattgtag tgaagaatct agttgcgaag agagagaatg tataaagcaa tttggtacca
    23761 atgaaatgcc acctcaacct cctaaagata ttcaatgtat aactataatt ggagaaattg
    23821 agggacattt tataggaaat cctcaaaaaa aagctacaaa gtacgagcat ataattccaa
    23881 tgctatactc aattgaggaa agtaatgatg ttaaaggagt gttagttgtt ttaaacactg
    23941 ttggaggaga tattgaagca ggtttagcta ttgcagaact tttaaacagt acatctaaaa
    24001 aagtagttac tcttgttcta ggaggaagtc atagtatagg tgttcctctt gctacagcag
    24061 gagattactc atttatagca ccaacagcaa ccatgattat acatccagtg cgaactactg
    24121 gtcttgtgat tgggataaat gagacttttg agtattttaa aaagatgcaa gatagaataa
    24181 ttcaatttat aataagaaca tcaaatatca aaaaagatgt acttgaaaaa cttatgcatg
    24241 aaaaagatga acttgtaagt gatgttggta gtgtattaat aggaaaagaa gctgtggatt
    24301 atggtcttat agatgaagtt ggaggactaa aagaagcact taaaaaatta agagagctta
    24361 taaaagaatc tgaggaagaa aaaaataatg attaaaattt gtttaaataa taaaatttat
    24421 gatataatga tgtaataaag taggagtgtt agtgttaagg cattagcact ttttgctgtg
    24481 aaggggtaaa acttgctaaa aataaggagt gatattaatg gccacgaaaa agaagaaaaa
    24541 aaagaaggta aataccttaa gttttaatgc agaatatcat aatttaataa caatatttat
    24601 aggtgtattt ttattatata gtttaaattc taattctatg ggatggatac ctgtgttaat
    24661 gcaaaatctt tttaagggac tatttggagg gctttcaata gcaataccat ttatagtaat
    24721 tataacagga cttttaggat tttttgatgg aaatgaatat atatataggc taagaaagac
    24781 taaactatac tacataatta ttttatttat atttgtattt tatggattat taaatgcagg
    24841 aactcttcct gtagatagcc cactaaaagg taacatgttt gatgatgtta tgaaattggg
    24901 tgtaagtgga caaggtagtg gtttgatagc aacaactatt gcgtattata tgagtaaaat
    24961 atttggtata gcaggtggat ggttaataag tatcttcgca cttatacttt ctgtaatgtt
    25021 tatatttaat atttctataa aagatttggt gtcaaatgca aaatcaaaat ctaaagcatc
    25081 taaagacagc aatttaacgt ttaaagataa aattgcaaat atgaaaaaat ctgcaataga
    25141 tatgatgact gatgaagtag atgatactac tataaataaa aaaccaggtt tctttaaagg
    25201 gttaatgtca aaaggtctta ataatgatga tgaagaagat gaatattttg aagaagaaaa
    25261 tagtgatggt gtagatgata aaactataaa aatagttgga tttaataagg cagaagatga
    25321 gtatttagaa atattagaag gaactcaaag tatgccagag ttagatgttt taaaagaatt
    25381 acaaaaagct accaatgaaa atccagttgt agatactaaa ccagaaaaaa aagttgatat
    25441 agctaaacct aatttaaata tagaaaaaac tcaacctatg agtattgtag cagagccagt
    25501 taatgaggat tattcaaact ataaaaagcc tagcattgag cttttaaata aagtaaataa
    25561 gaagtctgat gaaaatggta aaaagaaagt gttgaaaaat gcttctcttt tagaaaagac
    25621 actatcagat tttggagtgg aagcaaaaat taatcaagtt acagttggac ctactataac
    25681 aagatatgaa atacaaccaa gtccaggagt caaggtaagt aaaattgtaa atttaactga
    25741 tgatatagct ttaagtttag cagctaaaag tataagaatt gaagccccaa ttcctggtaa
    25801 aagtgcaatt ggaatagaag ttccaaatga agaagctcaa atggttggtg tgagagaagt
    25861 tctagaaagt gaagaattta ataattttaa ctctcctctt gcaatgggac ttggaaaaga
    25921 cgtagcagga aaaattataa ttggagatat aggtaagatg ccacacttac taatagctgg
    25981 ttctacaggt tcaggaaaga gtgtgtgtgt aaatacatta ataagtagta ttttatataa
    26041 ggcaaatcct gatgaagtaa aattactatt aatagaccca aaagtagttg aacttgcaaa
    26101 ttataatgga ataccacatc tattgatacc agtagttact gaccctaaaa aagctgcaaa
    26161 tgctttaaat tgggcagtta cagagatgaa tagaaggtac aaattatttg cagatgctca
    26221 agtaaaagat gtaacaagtt ataatgagaa agcggaagaa aaacttccta aaatagtaat
    26281 aataatagat gagttagcag atttaatgat ggcaagtgca aatgatgtag aagattatat
    26341 ttgtagatta gctcaaatgg caagagctgc tggaatgcac ttgattgtag caactcaaag
    26401 accatctgtt gacgttataa caggggtaat aaaggctaat ataccatcta gaatagcatt
    26461 tgcagtatca tctcaaactg attcaagaac aatccttgat atgggtggtg cagagaagct
    26521 tctaggtaaa ggtgatatgt tattctatcc acttggtgca gcaaaaccag ttagacttca
    26581 aggggcattc atatcagaga gtgagtcgga aaaagtaatc gattttgtaa aaagtcaagt
    26641 taaagatgga ataaaatatg aagaagatat aattgaaact atatctaagg ttaatacttc
    26701 aaagggtagt gatgaggatg agtttttgag tgaagcgata gaatttgtcg tggaaagtgg
    26761 tcaagcatct gcctctatgc tacagagaag atttaaaata ggattcaata gagcagctag
    26821 acttatagat tctatggaag aaagaggaat aattggagca agtgagggaa gtaaaccgag
    26881 aaaggtatta ataagcaaac aggatttgca gaatttagaa ggtgaataga attgtcagtt
    26941 ataaaaatag aaaaagcact taagctagat aaagtaatat ttgtagatgt gagaactgaa
    27001 ggagaatatg aagaagacca tatattgaat gcaatcaata tgcctctttt taaaaataat
    27061 gaacataatg acgttggaac aatttataag atgcagggaa aacatgaagc aatacaaaaa
    27121 gggtttgact atgtatcgta taaattaaag gatatatatt tacaagcagc agaacttgca
    27181 ttaaattatg ataatattgt catatattgt gcaagaggcg gaatgagaag tggcagtata
    27241 gtaaatttat tatcatcatt aggtgtaaat gtatatcaac ttgaaggtgg atataaggct
    27301 tatagaaatt ttgtattaga gtatctaagg catgtaatgg atacaaaaaa ctttgtatct
    27361 gttcatggat tgactggtac aggaaagact gatttgttac atttattaga tgaaaaagga
    27421 atagatgtac tggatttaga aggcatagct aaaaacagtg gttctacctt tggttttata
    27481 acttttgata aaaagccacc ttctcaaaag aactttgaaa caaaactatt tgaaagtata
    27541 tttttttcga aagagaatta tatatttgtc gagagtgaaa gcaaacgagt tggaagtgtt
    27601 acagttccaa atgaaatttt cgaggcaatg acacgagaag gacatcatat attattagaa
    27661 tgtgatattg aaaatagagt tgatagactt tgtagagatt atatttatga taaagatgaa
    27721 ggtaatatat tgattttaaa agaatgtata aacaagttta gaaaaagatt gggtaataaa
    27781 gtagtagatg attatattga tttattagaa tcaggtgaat ataaagaact tgtaaaaaga
    27841 tatcttttag aatactacga cccactttat atgcattcag tagaaaaata taagtatagt
    27901 aagattataa attttaatga tacacaaaaa gctttagaag atgttataga cttatatgag
    27961 agtatattgg aaggagaaag tgaatgttaa aaatagcatt agaatcatta ggatgctcaa
    28021 aaaatttagt agatgcagaa ataatgatgg gaattttaaa taacaaaggc tataaattaa
    28081 ttggagattt tgaagaagct gatgtaatta tagtaaatac atgtggattt atagagtcag
    28141 ctaaacaaga atctatagat acgataataa actttgcaga gttaaaaaaa actggcaatt
    28201 taaagttgtt gatagtaaca gggtgtctag cccaaagata ttctgaagaa ttaaaaactg
    28261 aaattcctga aatagatgct atagtaggaa caggaagcta tcaaaatata gataaaatac
    28321 ttaaagaatt aagtgaaatg cataaaattg taagccttaa tgatatagag tttgtattta
    28381 atgaagattt accaagatat atatctacac ctagctatat ggcctattta aaaattggag
    28441 aaggatgttc caataattgt acttattgta taataccaaa actaagaggt aaatatagaa
    28501 gtagaaaatt tgaagatata attaaagaag ctaagaaatt agctgaatct ggagtaaaag
    28561 aacttgtggt tatagcacag gatacgacaa agtacggttt tgatttatat ggaaaagaaa
    28621 gattatcaga acttttagaa gaacttgcaa aaatagatgg atttaagtgg ataagggtta
    28681 tgtattcata tccagaatct ataacagaag aattgataca agttataaag aaatatgata
    28741 atatatgtag ttactttgat atgccaatac agcatgcaag taacaatatt ttaaaattaa
    28801 tgaatagaaa aactactaaa gaagatatat taaataaaat caatctgata agaagtaata
    28861 taccagatgc tatacttaga actacaataa tagtagggtt tcctggtgaa acagaagatg
    28921 acttcaaaca acttgtggac tttgttgaag aggttaaatt tgatagactt ggtgcatttg
    28981 catattcaag agaagaagat acaccagcag atagattgcc aaaccatata gatgaagaag
    29041 taaaaataca aagaagagat actttaatga tgattcaaca aaaaatatct gaagaactta
    29101 atgacaaaaa gataggaaaa acttatgagg tgttaataga agaacagata gaagataatg
    29161 tatatacagg aagaacacaa ggtgatgcag aagaaattga cagtatagtg tatgtaaaaa
    29221 gtgtagacaa tcttgaagta ggagaatttg taagtgttca aataaatgat gcaatggaat
    29281 acgatttaat gggagatgtt ttatatgaac ttgcctaaca agttaacttt gtttagaata
    29341 tttttaatac cagtatttgt tttaattatg ttgctcaata taccaaataa atttttaata
    29401 gcttgtatta tttttataat agcttcaatt actgatgctt tagatggaaa aatagctaga
    29461 aaatacaatc tagtcactga ctttggaaaa tttatggacc cattagcaga taagttattg
    29521 gttatttcag cacttacatg tatgatagaa gaccatcttg taagtagttg gatggtaatt
    29581 ataatagtgg ctagagagct tacagttagc atattaagag ctatagctgc tgctgatgga
    29641 aaagtaattg ctgctggaaa cagtggaaaa cttaaaacga taactcaaat ggtatctata
    29701 gtgtttttat tgttaggtgc tcagtttgaa aatgtattga tattaaatat tggtgaaatt
    29761 ctcattctta tagctacact acttacatta tattctggat gggaatatct atataaaaat
    29821 aaagagcttt ttatgtcttc taagtaaaaa attatgcttt aataaataaa aactaatgat
    29881 taataaataa aaaataacaa cttaaaattt taaaaatgta aaaaagggta tactattata
    29941 gtatacctct tttcatttta agaatatttt cttttgtgta tataattatt aataatggtt
    30001 tataaataaa atgtaatgaa taaaaatata atcattagta gcgtttttaa attgactagg
    30061 tttgatttat gttatataat taatatataa caagagaaca aatgtttgta gataaatgaa
    30121 gggatggtat agagatgagt gtagatcaag aaaaattaaa agcgttgaat gaagctttag
    30181 gtaaaattga aaaagatttt ggtaaaggtt cagtaatgaa attgggagaa gcaacatcta
    30241 tgtctataga tgttatatca acaggagcga ttggtttaga catagctatt ggtataggag
    30301 gtctacctag agggagaata gttgaagtat atggtccaga atcttctggt aagactactg
    30361 ttgcgcttag ttgtgtagca tcagctcaaa aagatggagg aatagctgca tttatagatg
    30421 cagaacatgc acttgaccca gtatatgcaa aagctttggg tgtggatgtt gataacctaa
    30481 taatatctca accagataca ggtgaacagg ctttagagat agcagaggca ttgataagaa
    30541 gtggagcgat agatataata gtaatagact cagtagcagc attagttcca aaggctgaaa
    30601 tagatggaga tatgggtgat tctcatgtag gtttacaagc tagacttatg tcacaagcac
    30661 ttagaaagtt aactggttca attaaaaaat caaattgtgt tgctatattt ataaaccagt
    30721 taagagagaa agtaggaata atgtttggaa acccagaaac tactactgga ggacgtgcac
    30781 taaaattcta ttcatcagtt agattggatg ttagaaaaat agatacaata aaacaaggtg
    30841 ataaagttat aggtagtaga actagagtta aagttgttaa aaacaaagta gcaccaccat
    30901 ttaagcaagc tgaatttgat ataatgtatg gagaagggat ttcaaaaatt ggagaccttt
    30961 tagatatagc tgctgatgta gatatagtta aaaaatcagg ttcatggtat agttacaatg
    31021 atactaaact tggacaagga agagaaaatg ttaaaaaatt cttggaagat aatttagatt
    31081 taactactga aatagatgag aaagttagag cattttacaa tttaaatgaa gaacatgaag
    31141 aatcaggtac ttcagtatca aaagaaattg tagaagaata agtaaaaata caaaaaaata
    31201 actagtaatt aaagacatgc cacgctccct attagcttga cacaatgttg aaaaaattat
    31261 aaaattaagt gaggataaaa tatctaaatt taattttaat tattaggaat attttttaat
    31321 catagtgatg acattttggg attattaacg aaggaggtgg atgtcataga tagtgtagta
    31381 atgattgtaa taggtgcagc tgttggtatt atagctggat attttgttag aaaaaatata
    31441 tctgaagcaa aaattggaca agctgaaaac ttggccaaag aaataataga taaagcacac
    31501 cacgactcag aaacagtaca aaaagaaaaa ttactagaag ctaaagaaga gatccataag
    31561 tggagaacag aagctgaaag agaaaacaga gaaagaagaa cagaagtaca aaagtatgaa
    31621 aaaagagtag tacaaaaaga agaagtctta gatagaaaat tacaaaattt agaatctaaa
    31681 gaagtaaacc taagtgaaaa attaaaggta gtggaaaaga aagaagaaga agttgaagta
    31741 ataaaaactc aacagttaga aaaattagaa agtatatcag gaattacttc tgataaggcc
    31801 aaagaaatca tcttaaccaa tgcggagaga gatgtaagac gtgaaatgtc tataatgata
    31861 aaagagatag aaagtcaagc taaagaagaa gctgataaga aatcaagaga aattatagga
    31921 tatgcaattc aaaaatgtgc agcagatcac gtagccgaaa ctactgtaac agtagtaagc
    31981 ttgcctaatg atgaaatgaa aggtagaata ataggtaggg aaggtagaaa tataaggact
    32041 ttagaaacct taacaggtat agaccttata atagatgaca ctccagaagc cgtaatttta
    32101 tcaggatttg atccaataag aagagaaatt gcaagaattg ctctagaaaa attaatagca
    32161 gatggaagaa tacatccagc tagaattgaa gaaatggttg aaaaggcgag aaaagaagtt
    32221 gacaatatta taaaagaata tggtgaacaa gctgcatttg aaactagtgt acatgggatt
    32281 catccggaat taataagatt attaggtaga cttaactata gaactagtta tggacaaaat
    32341 gtacttaaac attctataga agttgctcat atagctggta taatggctgc tgaaatagga
    32401 gcagatatta gattggctaa gagggcaggt ttattacatg atataggtaa ggctgtagac
    32461 catgaaatgg aaggtacaca tgttgaaata ggtatggatt tacttagacg ttataaagag
    32521 tctaaagaag ttatacatgc catgagtact catcatggag attacgagcc tcaaacagta
    32581 gaggctgtat tagttacagc agcagatgca atatcagctg ctagacctgg tgcgagaaga
    32641 gaaactttag aagcatatat aaagagactt gaaaaattag aagaaatagc taattcatat
    32701 gaaggtgtag aaaaatcatt tgctatacaa gctggtagag aaataagaat aatggtaaaa
    32761 ccagaagcta taaatgatga agaaatacat ctattggcta gagatatgac taagaaaata
    32821 gaggatgaac ttgaatatcc tggacaaata aaagtaagca taataagaga aacaagagct
    32881 atagagtatg cgaagtaaaa gaaataatat ctaaataata agaaaatatt tttagaaatt
    32941 taaatttgcc acaagatatg ataatatatc ttgtggcttt taaattgttt aagtttaatt
    33001 ttatataatg caaaaataat ctaagtgcta aaagtgctac ttacaaaaga ttaataaaat
    33061 aaagctttat atatgtatta tttatataat tatccaagtt tgtttgatat aatgaagttg
    33121 taaaataaat atattactac ttggaatgct tgtacttaaa taagatgaaa atacaatgac
    33181 gctaaagtga ggattagaga gagggtacac tatgaaaaaa aatacacttt tacatacaaa
    33241 tattatggtt tgcattatta ttactcttgg ttttgtggct acctctataa ttggatatca
    33301 atctaatacc aatattttta aaaatgatat tgaacatgta tctacattgg ctgctgaagg
    33361 tatttattat cagatagata aactactttc agaacctata aatgtatctc ttacaatggc
    33421 aaatgacagt ctattaaaaa attttttaga tggtgagaaa gaacatttaa atgatgaaga
    33481 atttatttat aaattacaag attatctgaa tgtgtataga aataaatatt catatgattc
    33541 tgttttttta gtatcaacta aaaccaataa ttattatcat tttaatggac ttgatagaaa
    33601 tcttagcgct aataactcag agaatcaatg gtattatact ttcttaaaga atgatgatga
    33661 atactcatta aatgttgata atgatgaagc aagtaacaat agtattacag tctttgtaaa
    33721 ttgtaagata aaagatgata atggagcaac tatgggaatt ataggtgttg ggttaaaggt
    33781 taattcatta caaatgttgc ttaaaggata taatgacaaa tttgatgttg ttgcacgctt
    33841 aattgatgat aaagggtttg tacaattagc agtagataaa accggtcatg agaatataaa
    33901 tttttttgaa aattctagta gcgacttgtc taattcgaaa caattaatat taaacaacaa
    33961 aaaggaacaa aagagttttt ggtattcatc tgaaaaatca aaaagttata ttgtatgtca
    34021 gtacatacct agtttaaaat ggcatcttgt tctagaaaat gattttactt taatgattaa
    34081 acagcttcat ctgcaatttt ttaaaggcat tgcagttata gttttaatta taatatttgt
    34141 actttttact attacacatg tgcttaaaaa atacaaaaac caaataataa agcttagtgt
    34201 ttcacagaaa ttggaatatc aaaaattgtt gagcaaatct actgaaggtc tttatgaaaa
    34261 tatttttgaa attgatatta ctaacaataa agctggttgt gaagatgaga ataggtattt
    34321 tcatgaattt ggattagggc ttaatacccc atatgatgaa gccttacata caatagctaa
    34381 aaaccaaatc aaagaagaat atgcacaagg ttatttagat acatttttac ctgaaaatgt
    34441 aattaaacgt tataatagcg gaattaataa tatcagctat gattttttaa ttaaaaaaga
    34501 tgaagaaagt tatcattgga ttagaattaa tgcacgaatt ttcttttgga catcagatga
    34561 atctgttcgt atgatatctt atcgtaaaaa tatagatgaa gaaaaaaata gagaattact
    34621 acttctcgaa cgttcacaac gtgattctct tacagggctt tacaataaag ctacaacaga
    34681 ggaatttatt cgttcatttt taaaaaatga aagtaatcaa gatttagaac acgtttttct
    34741 aattttcgat attgataatt ttaaaaatgt aaatgataaa tatggtcatg tatttggaga
    34801 ctttgttata agtgaatttg ccttagaatt aaaatctcaa tttcgtgagg acgatattgt
    34861 gggacgtatt ggtggagatg agtttgtagt attaataaaa gactttaata ataataacta
    34921 tgtgcttata gacaaattag agcgtttttg ttctaagatt aatcagaaaa aatttgttga
    34981 gcaagcagaa ttttctattg catgtagtat tggtgtagca atgtatcctg aacatggata
    35041 tgtatactct gaactatatg agaaagcaga ccaagcactt tactatgcaa aaagccatgg
    35101 gaaaaattct tatcatatat tttcagagga ttctattgat agtatttcat ttcatataga
    35161 ctctagagat ataaaagtat tattagaaat ggctactgat ggtgttgtca aatttgcttg
    35221 tatggaaaac ttaaaattac tttatttcaa ccaaaaaatg cttgaattta ctggatattc
    35281 agctcaaact gttacttcta taggttttga tattttttcg cctgtattac aagacgactt
    35341 acaaatggta ctagatacat taagagaagc aatttctaaa agagagactt ttgaaatagt
    35401 ttttcgtttt tgtcatacag atggtcatat ttttcctgta aggatgcaag gtaagtttgt
    35461 aagtgaatta tatcaaaatc gttatcctgt tttttatgcg atttatacag acatatcaag
    35521 tatcaatact ttactagaaa ataaatagta aatactacag taaaatctaa aaataggata
    35581 taatagtatt aatataagaa gattataaat aaaacctgc
//
LOCUS       DAMJYR010000031        34829 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.031, whole genome shotgun sequence.
ACCESSION   DAMJYR010000031 DAMJYR010000000
VERSION     DAMJYR010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 34829)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34829)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34829
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.031"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(441..2828)
                     /gene="cadA"
                     /locus_tag="PC471_001458"
     CDS             complement(441..2828)
                     /gene="cadA"
                     /locus_tag="PC471_001458"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HDF2788593.1"
                     /translation="MEANNSIKKEFILGGLNCAHCAEEINNKVSKLQEVKSSNLNFIN
                     KKLTVNIKESFNEDTTIEKIIDIIDSTEPGLDIQISSKENAASKTSIKKELILGGLNC
                     AHCAEEINNKVSKLKEVESSNLNFVNKKLTVNISNNFEEDDVINKIKEIINSTEPGLD
                     IQVGSTDKVKGRTTEKSGAVNDTNKKELIPLIIGALVYIFGIYQTATGYESQFSNIVF
                     IVAYVIVGGDVLLRAIRNISKGRVFDENFLMALATVGALAIGELSEAVGVMLFYKVGE
                     YLQGVAVGKSRKSITSLMQIRPDYANLKVNSEVKVVSPEEVNVGDIIVVKPGEKVPLD
                     GVVVDGVSMLDTSALTGESVLREVEKGDEILSGVINKNALLSIEVTKSFGESTVSKIL
                     DLVENSSIKKSKTENFISKFSRYYTPIVVIAALLIAFVPPLVISGEVFSDWLYRGLIF
                     LVVSCPCALVLSIPLSFFSGIGFASKNGILIKGSNYLEALRSVDTVVFDKTGTLTKGV
                     FNVTKLNPEGISEEELLEYAAIAEVNSNHPIAKSILSYYNKKIDLDTIDSYEEIAAYG
                     IRVKHNGNFILAGNEKLMKKENISYSSAKEVGTVVYIAVDKAYRGYIVISDEVKEDSK
                     NAIRSLKEIGVKEVVMLTGDNEKVAKNIAQELELDTVYSNLLPNEKVDRLEDLYEGRT
                     EKEKIAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVLMTDEPSKISKAIEIA
                     NKTNKIVWQNIIFALGVKIIVMILGAGGVATMWEAIFADVGVALIAVVNAMRAMR"
     gene            complement(2851..3216)
                     /locus_tag="PC471_001459"
     CDS             complement(2851..3216)
                     /locus_tag="PC471_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF2788594.1"
                     /translation="MREEINDCNCNIVHEEIVTEAKSTMPDEEMLYDLAELFKVFGDT
                     TRVKILYALFANEMCVCDIASLLNMTHSAISHQLRVLKQARLVKFRREGKTVYYSLDD
                     SHISQIFDCGLNHIRETYK"
     gene            3415..4263
                     /locus_tag="PC471_001460"
     CDS             3415..4263
                     /locus_tag="PC471_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2788595.1"
                     /translation="MSNKKKKDLDTSYMPYLDPANYADYITNEEIPQDELRNDPQISN
                     GYTKIPNNQNINNLYPNNVNHYPKSSNSPNNLPNTNNTSGNMNYNTSATNNMPNNMNN
                     NTGMPNNMSNNMNSNMGMPNNMPNNMNSNMGMPNNMPNNMNSNMGIPNNVPNNMNSNM
                     GMPNNMSNNMNSNMGMPNNMSGNMNNNMYGNTGMPNNMNSNMGYNMNNTMGIPNFSCN
                     QNMPPNVLMMIPGVICHNTMQGMPVVMPSTMPPNIYPTPYGSSNMSIQGIPQATNIEE
                     FDEEEM"
     gene            complement(4700..5788)
                     /locus_tag="PC471_001461"
     CDS             complement(4700..5788)
                     /locus_tag="PC471_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2788596.1"
                     /translation="MNFNFAKATNSRLMGSLGLIINWIDDENNHFCQYFLLDAEGLGL
                     ADYVSLNNPTQEEAYMEEERLMGGFGSDRVELTKDESLFLVSYFGNKNLYYDKLLPGD
                     KCEYIDIIKNYKTDLTIEKLYNKICKRVDEEVEFINYMTMRFIAWDRESLKYFSGSDE
                     IANMHITNINGTLLKNVVSDKGQGRYISEALYEDNDGYYICKIAFCISKCYETGFRIN
                     SLLVTDKEPMYDFEVFDEISKSEFVSVYSVNSPEEFAGVFYRENPFLLKSNMNEGIFF
                     TRFNFNNDHVKENVYIINNDMKAIYYLMGNKFFIGTYNENDCNYINEISQSNYSDYIK
                     FEEKFFFEQNALYDFAESGSLDFDDFLE"
     gene            5913..6641
                     /locus_tag="PC471_001462"
     CDS             5913..6641
                     /locus_tag="PC471_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family hydrolase"
                     /protein_id="HDF2788597.1"
                     /translation="MKNIAAFFDIDGTLYRDSLMVEHFKKLIKYDIIDQKNWYKHARD
                     TFMDWDKRQGNYDDYLLEICDLYVDSLKGLDKTCINFTSDQVIKLKADRVYKYTRSRI
                     EWHLNQGHIVIFISGSPGFLVEKMAKKYNVTDFLGSDYVFENNIFTGTVIPMWDSISK
                     NNAINDFVVKYDLDLSNSYAYGDTNGDINMLKRVGNPIAINPTKELLSQISLDEEISK
                     NVEIIVERKDIIYSLSTDVNIVDI"
     gene            complement(6843..7334)
                     /locus_tag="PC471_001463"
     CDS             complement(6843..7334)
                     /locus_tag="PC471_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2788598.1"
                     /translation="MSIKVAYGGVLLALNVILLTLTNIIPVNTLFIMGLASLLVSIVI
                     MEWGFKSGIAFYIGSIVLGFIVMASKSQWIVYSLTFGIYGIVKYLIEKDRSIYIEYFM
                     KLVFANIMILILYFLLRTIVYIPINIFIIGSFEIAFIVYDYVYSSFIGYYNNRLRKML
                     FKK"
     gene            complement(7345..7788)
                     /locus_tag="PC471_001464"
     CDS             complement(7345..7788)
                     /locus_tag="PC471_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2788599.1"
                     /translation="MYSNTYNNENFMRARAFIENSEFKKAYDFLKTLTDKCAEWYYLT
                     GFSAMNIGYYEEGEDFLKRAKFMEPENSEYSDALRSYTQYRNDYSNRADNYNRRRRND
                     LDGCCCCCCDDCCCCLGDDCCENCAKLWCLDSCCECFGGDLITCC"
     gene            complement(7778..8617)
                     /locus_tag="PC471_001465"
     CDS             complement(7778..8617)
                     /locus_tag="PC471_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2788600.1"
                     /translation="MFGYVKINKMDLTFREYDYYKAYYCGLCKYLKRNHGEISRFSLN
                     YDITFLIVLLTAVYNPESISTEEVCIVNPFKKKKVITNDITEYAASMNILLTYYKLED
                     NLMDDKRIKDKLAYYIYKNKLKLAYEKYPEKAEYIKQQLNELNKLEKDKNINIDEVSS
                     IFGNIMGEVFVYKKDENERNLRMIGFNIGKYIYLLDAYEDLDEDFKKGRYNPFIEYID
                     KNDELKEKVKKIIVTSLGFLARGIDNLNLNKNVGIIENIIYSGVYLRYINILESRGGK
                     NVQ"
     gene            complement(8836..10221)
                     /locus_tag="PC471_001466"
     CDS             complement(8836..10221)
                     /locus_tag="PC471_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HDF2788601.1"
                     /translation="MENLNKKFLMEYIDEIQGELRDISDKIWKNPELQYKEYYASNLQ
                     KEYLKKYGFSIDELEGMDTAFAASYGEGRPVIAVLGEYDALDSLSQKVSTIKEPIVDG
                     GAGHGCGHNLLGTGSIGAVIAVKELIKLKKLKGTIKYYGCPAEEDLSGKVLMIKKGFF
                     DGIDCAFSWHPFDINTPIRIPTLANYSVKFRYNGISAHAAQAPYNGRSALDAVELMNI
                     GCNYLREHIFDNCRVHYVTTNGGKMPNIVPDFAEVWYYIRGVKMEHVRDVFGRIVDIA
                     KGAALMTGTIMEYDIISGVYDYIPNTILTDILSQNMKLVGVQNYNEDDYYFADKLAET
                     VSIDKRSSVSSVLSGNKDITKMNLHDEVTDDTFTHNNCISLSLDIGDVSYIIPTAQCS
                     CSVWPIGISAHTWQSCASAGSDMGFKAMLLASKSISCSINDVMLDELVINKAKKELKD
                     TVGSFKYIPII"
     gene            complement(10394..11137)
                     /locus_tag="PC471_001467"
     CDS             complement(10394..11137)
                     /locus_tag="PC471_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytoplasmic protein"
                     /protein_id="HDF2788602.1"
                     /translation="MKKVLIVGESWVKNITHIKGFDTFVTTHYEEAVKWLKEAIESGG
                     YETVHMPAHVAADSFPYKLEELNEYDCIILSDIGSNTFLLSNSTFIDCNSNPDRLELI
                     KEYVNNGGALIMVGGYMSFTGIDAKARFGETAIKDVLPITMIDKDDRVEKPAGIIPEV
                     IDSKHPVLKGIPTEWPKFLGYNKTVARDNCPVLATIGGDPFVAVGEFGKGKSAIFSSD
                     CAPHWGPKEFTDWKYYNKLWVNMLDWLTC"
     gene            complement(11157..12419)
                     /locus_tag="PC471_001468"
     CDS             complement(11157..12419)
                     /locus_tag="PC471_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HDF2788603.1"
                     /translation="MKKILLDTLNSKKQEYIDYLKELVSIKTEDVGHGILGGFEKEGQ
                     EYIEKLANYIGFSVDRQEMSEELIKKAKNIYKEGNLGHNYQDRYNLICKYSDDLPGKT
                     IVFNGHVDTMPPGDISKWKYNPYRATEDNGKLYGLGTADMKSGLIASILAVKLIKDSG
                     LNVPGNVKIMSVVDEEGGGNGTINAVMNGIDGDCCIICEPSEQNLIVAHMGFVFFEVE
                     VKGVSLHCGSKWEGVNAIEKAMLLLQDIKELEHNWLMIYKHPLLPSPTINLGVINGGT
                     AGSTVPDKCVFNLCVHFLPNIMSYEQVVNDVTNVIMTRANGDLWLKDNKPNINIYQSG
                     LGFEMDKDSDFVVNAHKILEETLGKKLEIKGSTAGNDARLMKNLAEIPTLILGPGSIE
                     QCHSIDEYVEIKEYLDSILMYASLILNL"
     gene            complement(12603..13601)
                     /locus_tag="PC471_001469"
     CDS             complement(12603..13601)
                     /locus_tag="PC471_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HDF2788604.1"
                     /translation="MSSITKHKEEKSSPLLTKLTSVFRDQGSAAVGLVIIFIIMSVAS
                     SNFLTLDNLINVGRQISINAILAVGMTFVIITGGIDLSVGAVIALVGTFWATTVVNYN
                     APIWVGMILALAIGTILGVIKGAIISTQKLPPFIVTLAMLTIISGASFVFTGGRPISV
                     NTDAFKMLGRGYIGPIPIPVIIMIIVVIAGHFLLKRTNFGRHVHAVGGNEEAARLCGV
                     KVNKVIVKVYALAGLLTALAGIILSSRLASGSPTVGDGAELDAIAAVVLGGTNMMGGS
                     GSIVGTCIGVGIIGILGNGLNLLSVSSYNQMIIKGLVMLFAIWINNIKLKKSAKSK"
     gene            complement(13585..15096)
                     /locus_tag="PC471_001470"
     CDS             complement(13585..15096)
                     /locus_tag="PC471_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HDF2788605.1"
                     /translation="MSNIILKLSNIAKEFPGVRALDNVNFELFHGEVHALLGENGAGK
                     STMIKILTGAHSKTSGKFIFEGKEIENISPDISKKIGINAIYQELTVFDELTVAQNIF
                     MGKEINGKVLTNDKKMNEEAKKIFDNMGIDINPNSLVKELSIAQKQMVEIARVLSSET
                     KVLIMDEPTSSISKKETEILFRLINDLKESGVSIIYISHRMEELFEICDRITIMRDGK
                     TISTLNTKDVSSEEELVNLMIDRKLDQFFPKRKVEIKEEIMRVENLTKNNVFNDISFN
                     IRKGEILGIGGLVGSKRSEIVEAIFGLRTYDSGKIYLNNEEVKFKTPSDAIENGLGLI
                     TEDRKGTGLFLQMSVKENTTMAGLKKISKFKSIIDRKKEKEILEKYIEALKIKTPHMN
                     QVIQSLSGGNQQKAIIARWLLLQPDILIMDEPTRGIDVNAKAEIYNLMGDLVESGVSI
                     IMISSEIPELISMSDRIMVMREGHISGFLEGEEMVENNVLKLAFGGKINEFNN"
     gene            complement(15150..16112)
                     /locus_tag="PC471_001471"
     CDS             complement(15150..16112)
                     /locus_tag="PC471_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HDF2788606.1"
                     /translation="MRVRKILSLGIAGILAIGMLTGCSMEGPSKSDNKGGSDKKDLTI
                     GVSTITLQHQFFIDIDEGIKEKAKELGVKVIVNDPDQDVAKQTSAIEDFIQQNVDGMI
                     VLGTDNSAIVPAVEGAFEKMPVVTVDAVLNTENITSYVGTVSYDAGKKLGEYTKKYID
                     EKLGGKSEIAIVTDLKSQIQMQRIDGFKDALKGSANVKILNSQPGYDREESLNTVENL
                     IQSNPDVDIIYATAENSVLGAKAALESAKNKDVKIVGFDLTEEASSGITDGTILAMIQ
                     QQPKEMGRLAVEAVVKAIKGEKVEKNIPVPALLYDKENIKDFKN"
     gene            complement(16160..17050)
                     /gene="rbsK"
                     /locus_tag="PC471_001472"
     CDS             complement(16160..17050)
                     /gene="rbsK"
                     /locus_tag="PC471_001472"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HDF2788607.1"
                     /translation="MGNIVVIGSVNMDMVCSVDKRPEKGETVLGNSFFTSPGGKGANQ
                     AISASKLGANVKMISCIGEDGLGEELIRNFRTNKVDYSLVSRNNHKSSGVAVITLCEN
                     DNSIVVVPGTNELVDIELIKKNEEEIKNADIVLLQLEIPLKTINYVVNFCFENRIRVL
                     LNPAPAVKLNEDIIEKVTYLTPNEHEYKIVFDTNEGIEEVLKKYPNKLVITEGKNGAR
                     FYDGEEIKHVSCISVDVQDTTGAGDTFNGALAVAITEGKNLYTAVEYAVVVSGLSVTK
                     LGAQSGMPYKEDVEKYLNNK"
     gene            complement(17092..18105)
                     /locus_tag="PC471_001473"
     CDS             complement(17092..18105)
                     /locus_tag="PC471_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDF2788608.1"
                     /translation="MSITIKEIGELAGVSKTTVSKVINNKDENISQATREKILKIMKE
                     KNYVPNKLAQSLVTKKTNTIGLLIPDIRNPFFTDVSRGVEDKANEEGYNIILCNTDED
                     AKKEYEGIRTLSERMIDGIIFAASSNTNWKEANYKDIKIPTVLIDKKISMNKETLKGI
                     VKINNFEGAYIATKHLLDIGNKKIIYLSGPLQNEIAVDRLEGYKKALIECNLSYNPAY
                     VFEGKYKIEWGQEFIKNLEKIDFDAIFCANDLIAIGVIRGLKERGLSIPNDISVVGFD
                     DIQTSSLISPSLTTVKQPSYDIGYKASEILINCLRGDKKESFDELIFKPELVIRDSTK
                     ESE"
     gene            complement(18334..19311)
                     /gene="bioB"
                     /locus_tag="PC471_001474"
     CDS             complement(18334..19311)
                     /gene="bioB"
                     /locus_tag="PC471_001474"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HDF2788609.1"
                     /translation="MEKYIIKLKNKVLREKEISYEEALNLISLDTNNKNDFDILLKSA
                     NEIREYFMGRKADLCTIMNAKSGKCSEDCKFCAQSSHYKTGVEEYSLLDYNEILNRAK
                     EMESKGVHRFSLVTSGKGMSGKEFNNILNIYEGLRENTNLKLCASLGIIDYEQAKMLK
                     SAGVTTYHHNVETCRDNFHNICTTHTYKDRIKTIKDAKKAGLDVCVGGIIGMNESEEQ
                     RLKMAFEIRELNVKSFPINILNPIKNTPMENYDVLEPMEILKTTAVFRFIIPNVYIRY
                     AGGRLSLKGYDKVGFNGGVNSAIVGDYLTTVGSGIENDKKMIIEQGFEL"
     gene            complement(19517..19780)
                     /locus_tag="PC471_001475"
     CDS             complement(19517..19780)
                     /locus_tag="PC471_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2788610.1"
                     /translation="MKIYYEKENSKKANVNNIPSVFAFNFLRNFLTSKEYEETRKEYF
                     INNLTKSQVNQAMKDLKWLFREYEGLEVITMESLDGVKTRIVL"
     gene            complement(19850..20923)
                     /locus_tag="PC471_001476"
     CDS             complement(19850..20923)
                     /locus_tag="PC471_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HDF2788611.1"
                     /translation="MEKSKVYFCDLYSNSQNKNVPNNVRRLFDEAGFKDLIEKNDQVA
                     IKLHFGEKGNTTYMSPVAVRQVVDKVKDCEGKPFLTDTNTLYTGSRTNSVDHLTTAIE
                     NGFAYAVVNAPVIIADGLYSRNYENVKIDKKHFESVKIGGEIYNSSAMIVMSHFKGHE
                     AAGFGGALKNLAMGCASAAGKQMQHSDVTPVVKEKKCVGCGKCVNSCPTKAISIVDKK
                     AVIDSDVCYGCGECPTVCPTRAVTIQWESDSDVFVEKMAEYAYGAVSNKKDKVGYITF
                     VMNVTPLCDCVPWSGRPIAHDIGILASTDPVAIEQACYDLICKEMGHDVFKHEHPHVN
                     GTRIIDYACEMGMGSKEYELIKL"
     gene            complement(21171..21929)
                     /locus_tag="PC471_001477"
     CDS             complement(21171..21929)
                     /locus_tag="PC471_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2788612.1"
                     /translation="MREYIAFTKKEFKENLRNYKLFSLIILFLIFGISSPISAKFMPD
                     LIAHFAPTLKVTAAPTALDSWTQFFSNISGLGMSLTLIIFCNILSNEYSKGTLVIMLT
                     KGLSRSSIVLSKFSVTVIIMTIGFWLSFLFAYGYTIYFWPTANLNHIIFSAFNLWLIG
                     IMYISVLILGCVLFRPAFASVLLVLVATLLLSLISIPKQIAPYTPNFLMSKNIDLISG
                     KVAMPEFIIPIIITVLISVVCLLAAVILFNKKPV"
     gene            complement(21926..22843)
                     /locus_tag="PC471_001478"
     CDS             complement(21926..22843)
                     /locus_tag="PC471_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2788613.1"
                     /translation="MNIVSINGLSKGFGNRKIIDNLNFTVPEGSVFGFVGKNGAGKTT
                     TMKMVLGLLKPDSGTIDVCGEKVTYGKTSSNRHVGYLPDVPEFYNYMRPLEYLSLCGE
                     ITGLSKKEIQIRSEELLSLVGLGNEKRRIGGFSRGMKQRLGIAQALLSRPKLLICDEP
                     TSALDPVGRKEILDIMLKIKDSTTVIFSTHILSDVERICDHVAILNKGSIALSGTLSE
                     IKSMHGKDRLLLEFASNDEIQKFKSSDGIKSLLKDSEETNMEIVLHGKDIKAIQKTVI
                     STLAEMNLCPVKMELIELSLENLFLEVVK"
     gene            complement(22843..23043)
                     /locus_tag="PC471_001479"
     CDS             complement(22843..23043)
                     /locus_tag="PC471_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLDc_N domain-containing protein"
                     /protein_id="HDF2788614.1"
                     /translation="MENLMEYLPLLVPVIILDLILIITALVHVLRHPNYKIGNKAIWI
                     IVVLFISLIGPILYFTIGRGEE"
     gene            complement(23048..24142)
                     /locus_tag="PC471_001480"
     CDS             complement(23048..24142)
                     /locus_tag="PC471_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2788615.1"
                     /translation="MNELNIAKTLILKRKEKGITQDELANYIGVSKASVSKWETGQSY
                     PDITFLPQLATYFNITVDELICYEPQMMKEDINKLYNKLCKDFTVKPFDEVMDEIREI
                     IKKYYSCFPLIFRMGLLIVNHYDIVDEKKRELLISEALEIFIRIQETCNDIDICRQAK
                     SMEATCYILLNQPIQVIDLLQNSNFPMINESILLAQGQMMNGQMDEARETFQLGAYQN
                     LISLVQNLVGILQNADKLQMKEIERRILAISDIFELDTLSPAIMLSSYLTQAQINLIH
                     GDNEGAIKSLRKYVDLATRDIYPIIIHGDDFFNKLDRWISEIGTGITRDDTIVKAGIV
                     AAIKNNPMFSVLSENKEYKFLIEKLSLLEE"
     gene            complement(24652..24792)
                     /locus_tag="PC471_001481"
     CDS             complement(24652..24792)
                     /locus_tag="PC471_001481"
                     /inference="COORDINATES: protein motif:HMM:NF021468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2185 domain-containing protein"
                     /protein_id="HDF2788616.1"
                     /translation="MNGEGCILSNKITKECYKVGYMIREKSIDTYPDSGWGFMTWDEN
                     GE"
     gene            complement(25102..26409)
                     /locus_tag="PC471_001482"
     CDS             complement(25102..26409)
                     /locus_tag="PC471_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HDF2788617.1"
                     /translation="MDISRSVDNSLNRTIEFLKELIKIDSQQGEPISQCPFGIGPKKS
                     LDKTLDYCASLGFSVKNIDNYIGYAEIGEGEELIGIPMHLDIVPPGEGWSVDPFSGAV
                     IDNIIYGRGVIDNKGAVSMLIHVLKNIEDMYPTINKRIRLIFGTNEETGMKCIKYYLD
                     KGEEIPSMGFTPDAMYPVVNGEKGRVHIRIEKEIKIDKSKPYIIVRGGTKENVVPSHC
                     TAKIINGIISELTTKGVAVHASNPEKGENAISKMVIKIVEDNMDFQHREDIELVSKYL
                     CSDYYGDALGINQYDEVFKNTTLNLGILKVNEEKIVCELDIRYGKNIVLNNIIDRFKK
                     VFCNGWKIEVIAHKDLHYVDESNLVLKKLLEAYEEVTDENGYTIAMGGGTYASWFKDM
                     VAFGPKFLAYKTGGHGVDERVPINHIRKNMEIYTLALIKLLEL"
     gene            complement(26474..26953)
                     /locus_tag="PC471_001483"
     CDS             complement(26474..26953)
                     /locus_tag="PC471_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-amino-thiazoline-4-carboxylic acid
                     hydrolase"
                     /protein_id="HDF2788618.1"
                     /translation="MSEKMYTEKEYLEGIRGATGERAVWFYLLMKEAEKLGANPDDIC
                     KEAIYGFGKMRGQKYNVADTPGKMAEMLYNSKGQKVFEMELVENTDENGVLKFHHCPL
                     DAAWKEYGLTKEERKEICRLACYGDYGRVDCAQGVKLDFAQKCAHDDEVCELVFTKK"
     gene            complement(27000..27863)
                     /locus_tag="PC471_001484"
     CDS             complement(27000..27863)
                     /locus_tag="PC471_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HDF2788619.1"
                     /translation="MQEIKNKEVKNGQGPTEVNKTRVLTKKDVTKTYLRWWWTAELSN
                     SFERMQALAVCASFTPALEKLYKRKEDLVDALKRHLQFFNTQAIWGGLIHGTVLAMEE
                     EKATEGKIPGEVISGVKNGLMGPLAGIGDTLDFGTFQTIFLALGASFGAEGSVIGAFF
                     PIMFSILLFCEGYYLFHLGYSLGRDSIKKILSGGIVNKIIDGASILGMFMMGALSATT
                     VKLSTPLSFDIGGKAIVVQDTLNMIAPGLLPLGVVFFVYWGMKYKKWTITKLLVILVV
                     LALVGSFIGIF"
     gene            complement(27863..28618)
                     /locus_tag="PC471_001485"
     CDS             complement(27863..28618)
                     /locus_tag="PC471_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDF2788620.1"
                     /translation="MESVLMLAIVTGLWYWFAAGLAGYTLFSTLKSPLFIGFSLGLLW
                     GDVTTGMIVGASIEMVYLGMVAAGGNIPSDKCLAALIAIPVALQTGVNAEVAVSIAVP
                     LGVIGVLVNNLRRTGNAVLVHKADKYAEEGNTKGIWRCATLYSLIFGFVLRFPIVFVC
                     NFFGADLVQSLLDVIPQWLMNGLTVMGGILPALGFATTIFTIGKNKFLPMFIIGFFMV
                     QYFEISITAAAIFGVCIALLITFMKEDKRVGEV"
     gene            complement(28645..29133)
                     /locus_tag="PC471_001486"
     CDS             complement(28645..29133)
                     /locus_tag="PC471_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDF2788621.1"
                     /translation="MAKITLVRVDHRLIHGQVITKWVKIAQAQKIIIVDDFLGQDEFM
                     ADIYKMAAPSGVEVVILTAEDAGQAFQNNTLGDKNIFILFKNVDMANKAYKAGLKYEK
                     IQLGGIPNEAGKKMVFTAVSLGNEDVEQLNELNENGVEIVLQVIPEESSMTYENALKK
                     FK"
     gene            complement(29151..29552)
                     /locus_tag="PC471_001487"
     CDS             complement(29151..29552)
                     /locus_tag="PC471_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIC"
                     /protein_id="HDF2788622.1"
                     /translation="MNKYKVIILCHGTWGKVLVEETNKNFGLSNQYEVLSLSSEKDVS
                     VFMKEVEEVVDKEDVKIIISDLYGGSTSSVAIAVAIRRGIDAINGLSLQTILIVDEEL
                     SKEDKLHTLPERIVKKNNNLCVDLIKEFKKI"
     gene            complement(29555..30052)
                     /locus_tag="PC471_001488"
     CDS             complement(29555..30052)
                     /locus_tag="PC471_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDF2788623.1"
                     /translation="MSLVKYCRCDDRLIHGQVIYKWVKHLGVKKIVVVDDETTNDVIA
                     KGLIKMAAPKNIDLSILTVSESRRYFYNNQADDNVFVLIKNLDTANRMIEEGINIKKL
                     IVGRIPTGIGKKKISQNVYINKKEFLLIDEFIKKNINVSIQMVPDEEEVELNQNINRY
                     KGEFI"
     gene            complement(30076..30480)
                     /locus_tag="PC471_001489"
     CDS             complement(30076..30480)
                     /locus_tag="PC471_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDF2788624.1"
                     /translation="MIGIIVMTHGSFSEEIIKSCELIAGPAERTAAIKLNRNDNIEDL
                     NKNFVEKLNELDEGDGVLVLADLLGGSPSNVASLNLKKGGKFHALTGVNLPMLLEALI
                     NREGKSLEELAEACIEAGQTGINNINKVLSSM"
     gene            complement(30710..33277)
                     /locus_tag="PC471_001490"
     CDS             complement(30710..33277)
                     /locus_tag="PC471_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDF2788625.1"
                     /translation="MTQKKLSRKEKVLICLKRLTEEHLNFNNGIRFGFDAEYIATEVG
                     INRSNASKELNQLIKESIIIKIKGKPVQYLYKESIESILGKKLNKGIFDNINSINEYS
                     ENDSIKDEYILESIKKENNIFNLVGCDGSLRSQIEQAKAAVIYPPKGLSTLIIGPTGV
                     GKSIFAEYMYKYSLTIKEDSDNAPFIIFNCADYSDNQQLLLAQLFGYVKGAFTGADKD
                     KYGIVHEANNGVLFLDEVHRLSAEGQEMLFLLMDKGIYRRLGEANKVHECRVMIIAAT
                     TEDPETAMLETFLRRIPVTIKIPSLEERGFQERMKLICYFFKEESVRIGVKLKVSKEV
                     IKAFILYKCKGNIGQLKSDIQLICARAFLDYMTYKRECVYVRLSLLPNNIRESLYGNN
                     RKEEFVQNFSFIGKDDIIFLPEEKDFESENLLIENKKYDLDFYGMIKETWNKLQQEGI
                     KESQIRSVIDGDIQKYSYNLMNTFIYNSSNQTAYNNIVNDSIATTVKYILQKHDKWSK
                     REGLDKLIKAIALHIQNLIERIKIGNIVKHPNKDEIMKERAYEYNIAKEILSNMSLMY
                     GVEFPEDEAIFLATFLYLSYVGLNEESIAILVIMHGESTASSMVNVANTLLDCNHAVA
                     INMGLEDRVQDILVQAVEIANKIDKGKGILILTDMGSILTFAKVIRDATGKEVKAIDM
                     VSTPIVIEATRKALTPEMTLEKLYFNIIESIKKHYGDMEVTYAEKDNGGRYFDRLLID
                     NISKTLTFLNGEKAYFILKEVINRISEHYSLVIQDELLVKFIFHCSCMIERVIIKESL
                     VYKSYEERISRNKELYLVIKESFKLVEETFDIIISDMEYANILDIFESQYDTDKFKS"
     gene            33443..34186
                     /locus_tag="PC471_001491"
     CDS             33443..34186
                     /locus_tag="PC471_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase"
                     /protein_id="HDF2788626.1"
                     /translation="MNFLSDLHTHSIVSGHGYSTLLENINYCKENGIKILGTSEHGPK
                     MPGSPHRWYFHNIQNIPRIINNIIILRGCEANILDIKGNIDLEPFVIPRLDYLILSFH
                     EAVFSPNTLENNTKALINAINKHDNIEILGHLGNPNYPIDYELIIKLAIEKNILIEIN
                     NCSIKGVSRNGSSDNCKYIATLCKKYGAKIILTSDAHICFDIGNYEYSENILKEINFP
                     DELIMNYPKKLINHFHQKGKLLDVDYTNI"
     gene            complement(34498..34704)
                     /locus_tag="PC471_001492"
     CDS             complement(34498..34704)
                     /locus_tag="PC471_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2788627.1"
                     /translation="MSKYSSLWEYVKKNNSQSFKLTFEEIKDIAGIEIDHSFLKYKKE
                     LNEYGYQVGKISLKEKTVIFDKIG"
BASE COUNT        11276 a         6376 c         3557 g        13620 t
ORIGIN      
        1 cttaaaaata attcgtcaat acttttttaa attatttttt aaaaatattt actttcttat
       61 attacataat ttattgaatg gcagtcatac tgttggttta gagtatttta acacacacat
      121 tttgttgtaa aaattacaac tattaatata atatatatac aatcataagt attataattt
      181 ttatgaatct aatttaaact taatataaac ttattaataa accttctatg tctaaatttt
      241 aaaattacaa tataaaagct ctacactaaa taaatacatt aaataaacat aatggataat
      301 gaatataaat ttatatatgc aaagcgctcc aataaaaagg taccaataaa gttaaaagaa
      361 acttattgat acctttcatt attacatact tcaatatata attataagtt ttttaaatag
      421 tatggattta catttatccc ttatctcata gctctcatag catttactac tgctataagt
      481 gccacaccta catcagcaaa tatagcttcc cacatagtag ctactcctcc agcacctagt
      541 atcataacta taatttttac tcctagagca aatattatgt tttgccacac tatcttatta
      601 gtcttgtttg ctatttcaat tgctttagat atcttgctag gctcatctgt cataagcact
      661 acatctgcag cttcaatagc agcatctgaa cctaagcctc ccatagctat accaacatca
      721 gcacgagcta atactggagc atcatttatt ccatcaccta caaatgctat tttctctttt
      781 tcagttcttc cttcatataa atcttctaat ctatctacct tttcattagg aagtaaattt
      841 gagtaaactg tatccaattc taattcttga gcaatatttt ttgctacttt ctcattatct
      901 ccagttaaca ttacaacttc ttttacccct atttctttta agcttcttat ggcattttta
      961 ctatcttctt ttacttcatc agagataact atataacctc tatatgcttt atcaacagca
     1021 atatagacta ctgtgcctac ttctttagct gatgaataag atatattttc ttttttcata
     1081 agtttctcat tacctgctaa tataaaattg ccattatgct ttactctaat tccataagct
     1141 gctatttcct cataactgtc tattgtatct aaatcaattt ttttattata gtaacttaat
     1201 atggactttg ctattggatg atttgaattt acctccgcaa tagctgcata ttctaataat
     1261 tcttcttctg atataccttc aggatttaac ttagttacat tgaacacacc tttagtaagt
     1321 gttcctgttt tatcaaatac tactgtatct acacttctta aagcttctaa atagttactt
     1381 cctttaataa ggataccatt tttagatgca aacccaattc cactaaagaa actaagtggt
     1441 attgataaaa ctaatgcaca agggcaagaa acgactaaga atatcaatcc tctatataac
     1501 caatcactaa atacctcacc tgagattaca agtggtggca caaatgctat taataaagca
     1561 gctattacta ctattggagt atagtatctt gaaaacttag atataaagtt ttctgtcttt
     1621 gattttttaa tgctcgaatt ttcaacaagg tcaagaatct tagaaactgt tgattctcca
     1681 aaactctttg taacttcaat actcaataaa gcatttttat ttataacacc tgataagatt
     1741 tcatctcctt tttcaacttc tctcagcact gactctcctg tgagtgcaga agtatctaac
     1801 atagaaactc catctactac tacaccatct aatggtactt tttcaccagg ttttactact
     1861 attatatcac ctacatttac ctcttctggt gacaccacct taacttcaga attaactttt
     1921 agatttgcat agtctggtct tatttgcata agtgaagtta tagactttct ggatttacca
     1981 actgcaacac cttgtaaata ctctcctact ttatagaaaa gcataacccc tactgcttct
     2041 gataactccc ctattgctaa tgctcctact gttgctagag ccatcaaaaa gttctcatca
     2101 aatactcttc cttttgatat atttcttatt gctcttaaaa gcacatcacc acctactatt
     2161 acataggcaa ctataaatac tatattgcta aactgacttt cataacctgt agctgtttga
     2221 tatataccaa atatatatac aagtgctcca attataagtg gaattaattc tttcttattt
     2281 gtatcattta cagctcctga tttttcagta gttctccctt ttactttgtc agtagaccct
     2341 acttgtatat caagccctgg ctctgttgaa tttattattt cttttatttt atttataaca
     2401 tcatcctctt caaagttatt acttatattt actgtaagtt ttttatttac aaagtttaaa
     2461 ttagaagatt caacttcttt taatttagaa actttgttat taatctcttc tgcacaatga
     2521 gcacagttta atcctcctaa tataagctct tttttaattg atgttttact agctgcattt
     2581 tctttagaac ttatttgtat atcaagacct ggctctgttg aatctattat atctattatc
     2641 ttttctatag tagtatcctc attaaaactt tcctttatat ttactgtaag ttttttattt
     2701 ataaaattta aattggaaga tttaacttct tgtagtttag aaactttatt attaatctcc
     2761 tctgcacagt gagcacaatt taatcctcct agtataaatt cttttttaat tgaattattt
     2821 gcttccatat atattcactc ctctcattta tcatttataa gtctctctaa tatgatttaa
     2881 tccacaatca aatatctgac ttatatgact atcatctaat gagtaataca ctgtttttcc
     2941 ttctcttcta aactttacta atctagcctg ctttaatact ctaagttgat gagatattgc
     3001 agaatgagtc atatttaata aactcgctat atcacataca cacatttcat ttgcaaataa
     3061 tgcatataat atctttactc tagtagtatc cccaaatact ttaaataatt ctgctaaatc
     3121 atataacatt tcttcatctg gcatagttga tttagcctct gttactattt cttcatgtac
     3181 tatattacaa ttacaatcat ttatctcttc tctcattttt aatcctccat tattttaata
     3241 tatgaataac tgttcatatg ttcattatat gttatcattt ctaaatttgc aacaataatt
     3301 tttattaatt taatataaat agaatgcaca tattataaac ttttttaata tatatattat
     3361 tagaataagt ttttgtaaac ttttttatag ttttctagga ggtgcaagtt taatatgagt
     3421 aacaagaaaa aaaaggattt agatactagt tacatgcctt acttagaccc agcgaactat
     3481 gcagattata ttactaatga agaaattccc caagatgaat tgagaaatga ccctcaaatt
     3541 tctaatggtt atacaaaaat acctaataac cagaacataa ataacctata tcctaataat
     3601 gtaaatcatt atcctaaaag tagtaattct ccaaacaact tgcctaacac aaataataca
     3661 tccggaaata tgaattacaa tacatctgct acaaataata tgcctaataa catgaataat
     3721 aatacgggta tgcctaataa tatgtctaac aatatgaata gtaatatggg tatgcctaac
     3781 aatatgccta ataacatgaa tagtaatatg ggtatgccta acaatatgcc taataatatg
     3841 aatagtaaca tgggcattcc taacaatgtt cctaataaca tgaatagtaa tatgggtatg
     3901 cctaacaata tgtctaataa tatgaatagt aatatgggta tgcctaacaa tatgtctggc
     3961 aatatgaata acaatatgta tggtaataca ggtatgccta ataacatgaa cagtaatatg
     4021 gggtataata tgaacaatac tatgggtatt cctaacttct catgtaatca aaatatgcct
     4081 cctaatgtac ttatgatgat accaggagtt atatgtcata atacaatgca aggtatgcct
     4141 gtagttatgc ctagtactat gcctcctaat atatatccta ctccttatgg ttctagtaat
     4201 atgtctatac aaggtatacc acaggctact aatattgagg aatttgatga ggaagaaatg
     4261 taatatatat ttttttaagt aagttgtata aaacattaat aattgaccat aataataaat
     4321 gtattcctca taatattccc ccaatattat tataggtata gaaagttgac tgaatatgcg
     4381 cttattcggt caacagatat taacataatc taataagata aacatagaaa aagtcagata
     4441 aaatttaatt ttatctgact ttttcattta aaaatgctct cttgtattgg tgggtcagaa
     4501 tagtatgata ctcgtatttg agactaatat tggcaaacaa cataaagaaa aatataaatt
     4561 ttttataata aactgtataa aattttaata attgaccaca ataataaatg tattcctcat
     4621 aatattcccc caatattata gaaataaatt taagcgagat taaattcagt taatctcgct
     4681 taaataatgt ctaaatttat tattccaaaa aatcatcaaa atctaatgaa cctgattctg
     4741 caaaatcata taatgcattt tgctcaaaga aaaatttctc ttcaaattta atatagtcgc
     4801 tataattaga ttgacttatt tcattgatat aattgcaatc attttcgtta taagtaccaa
     4861 taaaaaactt atttcccatt aagtaatata tagctttcat atcattattt attatatata
     4921 cattttcttt tacatggtca ttattaaaat taaatctagt aaagaatata ccttcattca
     4981 tattactttt taacagaaat ggattttctc tataaaatac tcctgcaaat tcttcaggtg
     5041 aatttacact gtaaacactt acaaattcac ttttagaaat ctcatcaaat acctcaaaat
     5101 catacatagg ttctttatct gtcactaata gagaattaat tctaaaacca gtttcataac
     5161 acttacttat gcaaaaagca attttacata tataatatcc atcattatct tcatataaag
     5221 cttcacttat atatctgcct tgacctttat cacttaccac gtttttaagt aatgttccat
     5281 ttatattagt aatgtgcata tttgcaatct cgtcacttcc agaaaagtac tttaaacttt
     5341 ctctatccca tgctataaat ctcatggtca tatagtttat aaattcaact tcctcgtcaa
     5401 ctcttttaca tattttattg tataattttt ctatagttaa atctgtttta tagtttttta
     5461 taatatcaat atactcacat ttatctcctg gcaatagctt atcataataa agattcttat
     5521 taccaaaata agatactaaa aataaagatt catccttcgt taattcaact ctatctgaac
     5581 caaatcctcc catcaatctt tcttcttcca tataagcttc ttcttgtgtt gggttattta
     5641 aactcacata atccgctaat cctaatccct ctgcatctaa taaaaaatat tgacaaaagt
     5701 gattgttttc atcatctatc caatttatta taagacctaa actgcccata agtctagaat
     5761 ttgtagcctt tgcaaaatta aaattcatat agtcacatcc tttaaactct aaaatacttg
     5821 ttttttaata tataaattat tatacatata ttttaacaac ttagatacaa tttataatat
     5881 aatataagga aatgattaat tgaggtggtc ttatgaaaaa tatagcagct ttttttgata
     5941 tagatggtac tctttataga gactctctta tggttgaaca ttttaaaaaa ttaattaaat
     6001 acgatataat tgaccaaaaa aattggtata aacatgctag agacactttt atggattggg
     6061 ataaaagaca aggtaattat gatgattatc tattagaaat atgtgacctt tatgttgatt
     6121 cactaaaagg attagataaa acctgtatca actttacaag tgaccaagta attaaattaa
     6181 aagcagatag agtttataaa tacacacgtt caaggattga gtggcatcta aatcaaggac
     6241 acatagttat atttatatct ggtagccctg gatttcttgt tgaaaaaatg gccaaaaaat
     6301 ataatgtaac cgatttttta ggaagtgatt atgtctttga aaacaatata tttacaggca
     6361 cagttattcc aatgtgggat tctataagta agaataatgc tataaacgat tttgttgtaa
     6421 agtatgattt agatttatca aactcgtatg cctatggaga tactaatggt gatataaata
     6481 tgctaaaaag agttggaaat ccaatagcaa taaatccaac aaaggaactt ttatctcaaa
     6541 tatcacttga tgaggaaatt tctaaaaatg ttgagataat agtagaaaga aaagatatta
     6601 tttattctct atctacagat gtaaatatag tggatattta atttatacta tctattttaa
     6661 aaacttatat tatatctaat atttatatct aacatataaa agctttgact aaaaatcaaa
     6721 gcttttatat gttgcactaa gaatatatta aatttagctt tatgctttaa tgtatattca
     6781 tatatatcat ttacacttat ctaaactaca atttcattaa aattaaatat aaaaatcaac
     6841 tattattttt taaatagcat ctttcttaat ctattattgt aataacctat aaaagaactg
     6901 taaacatagt catatactat aaaggctatc tcaaatgaac ctataataaa tatgtttatt
     6961 ggaatataaa ctattgttct taataaaaaa tacaatataa gtatcattat atttgcaaat
     7021 actaatttca taaaatactc aatatatata gacctatctt tttctataag atattttaca
     7081 ataccatata ttccaaaggt aagtgaatat acaatccatt gagacttact tgccatcact
     7141 ataaatccta aaactataga acctatataa aaagcaattc cactcttaaa gccccattcc
     7201 attattacaa ttgatactaa aagtgatgct aaccccataa taaacaatgt atttacagga
     7261 ataatatttg taagagtcaa caatatcaca tttaatgcta ataaaactcc tccataagca
     7321 acttttatac tcatatttat catcctaaca acaagttatt aaatctccac caaaacattc
     7381 acaacatgaa tctaaacacc aaagcttagc acaattttca caacaatcgt cacctagaca
     7441 acaacagcaa tcatcacagc aacagcaaca acatccatct aaatcattac gtcttcttct
     7501 attataattg tcagccctat tgctataatc atttctatat tgtgtataac ttctaagtgc
     7561 atcactatat tcactatttt caggctccat aaattttgct ctttttaaga agtcttcacc
     7621 ttcttcataa tatcctatgt tcattgcaga aaagccagtt aaataatacc actctgcaca
     7681 tttgtcagtc aaagttttta aaaaatcata tgctttttta aattcactat tttctatgaa
     7741 tgctcttgct ctcataaaat tttcattatt ataagtatta ctgtacattc tttccacctc
     7801 tactttctaa tatattaata tatcttaaat aaacaccaga ataaattata ttttctatta
     7861 ttccaacatt tttatttaga tttagattat caattcccct agctaaaaac cctaatgatg
     7921 ttactattat tttttttact ttttctttta actcatcatt tttatcaata tactctataa
     7981 atggattata tcttcctttc ttaaaatctt catctaaatc ttcataggca tctagtagat
     8041 atatatactt acctatatta aaacctatca ttcttaagtt tctctcattt tcatcttttt
     8101 tatagacaaa tacttcaccc attatatttc caaatatact agatacttca tctatattta
     8161 tatttttatc tttttctaac ttatttaatt catttagttg ttgttttata tactctgctt
     8221 tttcaggata cttttcatat gctaatttta atttgttttt atatatataa tatgccaatt
     8281 tatcttttat gcgtttatca tccattaaat tatcttccaa tttataatac gttaaaagaa
     8341 tattcatact tgctgcatat tctgtaatat catttgtaat tacctttttc tttttaaatg
     8401 gatttactat acacacttcc tctgtactta tagactctgg gttatatact gctgttaata
     8461 aaacaattaa aaatgttata tcataattca aagaaaacct agagatttct ccatgatttc
     8521 tttttaaata tttacatagt ccacaatagt aagctttgta ataatcgtat tctctaaatg
     8581 ttaaatccat tttgtttatt ttaacatatc caaacataaa tactcctttt tgtactattt
     8641 tttatttaga ttatatatag tataacactt tttatcaaga ttatataatt tacagtattc
     8701 taagaaactt aaaaaattcc ttctaatcat tgtatcttaa atttaaaata attagaaggt
     8761 tgggttaaaa ataatattaa attatatact tatattaact catacaagtt agttgtgctt
     8821 atatcaattt aagttttaaa taattggtat atatttaaaa ctacctacag tatccttcaa
     8881 ttcttttttt gctttattta taaccaattc atcaagcatt acatcattta ttgaacatga
     8941 tatagattta gatgccaata acattgcttt aaatcccata tcagaacctg cactggcaca
     9001 agattgccat gtatgggcag aaataccaat aggccaaact gaacaagaac actgtgctgt
     9061 aggaattatg taactaacat ctccaatatc caatgataat gatatacagt tattatgagt
     9121 aaatgtatca tccgtaactt catcatgcag attcatctta gtaatatcct tatttccact
     9181 taatacacta gaaacactac ttcttttatc tatagacact gtctcagcta atttatctgc
     9241 aaaatagtag tcatcttcat tatagttttg tacacctact aatttcatat tttgtgatag
     9301 aatatctgtt aatattgtat ttggaatata gtcatatact ccacttataa tgtcatattc
     9361 cataatagta ccagtcatta atgctgctcc ttttgctata tcaacaatcc taccaaatac
     9421 atctcttaca tgctccattt taactcctct tatgtaatac cacacttctg caaaatcagg
     9481 aactatattt ggcatttttc caccattagt agttacatag tgtactctac aattatcaaa
     9541 tatatgttct ctcaaataat tacaacctat attcataagt tctactgcat caagtgcact
     9601 tcttccgtta taaggagctt gtgctgcatg ggcactaatc ccattatatc taaatttcac
     9661 agagtaatta gcaagtgttg ggattcttat aggagtattt atatcaaatg gatgccaact
     9721 aaaagcacaa tcaattccat caaaaaaacc tttttttatc atcaatactt ttccagataa
     9781 atcttcctct gctggacatc catagtattt tatagtccct ttcagctttt ttagtttgat
     9841 taattccttt actgcaatta cagctcctat tgaacctgta cccagtaagt tatgtccaca
     9901 tccatgacct gcaccaccat ctacaatagg ttctttaata gtggatactt tttgagacaa
     9961 actatctaaa gcatcatatt ctcccaaaac tgctataact ggtctacctt ctccatagct
    10021 agccgcaaat gctgtatcca taccttctaa ttcatctata ctaaaaccat atttttttaa
    10081 gtattctttt tgtaaatttg aagcataata ttctttatac tgaagttctg gatttttcca
    10141 tatcttatct gatatatctc ttaattctcc ctgtatctca tctatatatt ccatcaaaaa
    10201 ctttttattt aaattctcca tattaattat ataaacctcc ctgtttctaa taacttatcg
    10261 attatcagcc aaaatgacta attttaactg atttaaaaat attttctatc atacaaccat
    10321 tttatacaat aaaacctctt gacttgtaag tttcatttta tcaagtaaga ggttttcaag
    10381 ttttataaaa tttttagcat gttaaccaat caagcatatt aacccataat ttattatagt
    10441 atttccaatc agtaaattct tttggacccc agtgaggtgc acagtcagaa ctaaagatag
    10501 cagattttcc ttttccaaac tcgccaacag ctacaaatgg gtctcctcca atagttgcta
    10561 aaacaggaca attatctcta gctactgttt tgttatatcc taaaaacttt ggccattctg
    10621 ttggtatacc ttttaatact ggatgtttag agtcaataac ttctggaatt attcctgctg
    10681 gtttttcaac tctatcatct ttatctatca ttgttattgg taatacatct ttgatagcag
    10741 tttctccgaa tctagctttt gcatctattc ctgtaaatga catatatcca cctaccatga
    10801 ttaacgcccc accattattt acatactctt tgattagttc aagtctatct ggattactat
    10861 tgcaatctat aaaagtacta ttagataata agaacgtatt agaacctata tcagataata
    10921 ttatacagtc atactcattt aactcttcta atttatacgg aaaactatct gctgctacat
    10981 gtgcaggcat atgaactgtc tcatatcctc cagattctat tgcttctttc aaccacttta
    11041 cagcttcttc ataatgagtt gtaacaaacg tatcaaatcc ttttatatga gttatatttt
    11101 ttacccaaga ttcccctact attaatactt ttttcattta tatattcctc ctacttttat
    11161 aagtttaata ttagacttgc atacataagt atactatcta aatactcctt aatttcaaca
    11221 tattcatcaa ttgaatgaca ttgttctatt gaacctggcc ctaatataag tgttggtatt
    11281 tcagctaaat ttttcattaa tctcgcatca ttgccagctg ttgaaccttt tatctctaat
    11341 tttttcccta gagtttcttc tagtatttta tgtgcattca ctacaaaatc agaatccttg
    11401 tccatctcaa aacctaaccc tgattgatat atgtttatat ttggtttatt atctttaagc
    11461 cataaatctc cattagccct tgtcattatg acatttgtta catcatttac aacttgttca
    11521 taactcatta tatttggcaa gaagtgtaca cataaattaa atacacactt atctggcaca
    11581 gtagaacctg ctgttccacc atttattaca cctaaattta ttgtaggtga aggtaatagt
    11641 ggatgtttat atatcataag ccagttatgt tccaactctt ttatatcttg aagcaacaac
    11701 atagccttct caattgcatt tacaccctcc cacttactac cacagtgaag agacacacct
    11761 ttaacctcaa cttcaaagaa tacaaatccc atatgtgcaa ctattaagtt ttgttctgat
    11821 ggttcacaaa ttatgcaaca atctccatca ataccattca taactgcatt tatggtacca
    11881 tttccgccac cttcttcatc tactactgac attattttta cattaccagg tacatttaga
    11941 ccactatctt ttataagctt tacagctaat attgatgcta taagtccaga tttcatatca
    12001 gcagttccaa gtccataaag ttttccatta tcttctgtag cacgatatgg attatatttc
    12061 cacttactta tatcacctgg tggcatagtg tctacatgac cattaaatac aattgtctta
    12121 ccaggtaaat catcactata cttacaaatt aaattatatc tatcttgata attgtgacct
    12181 agatttcctt ctttatatat attttttgct tttttaatta attcttcgct catttcttgt
    12241 ctatctacac taaaacctat gtaatttgca agtttttcaa tatattcctg tccttctttt
    12301 tcaaatccac ctagtattcc atgaccaaca tcttcagtct ttatacttac aagttctttt
    12361 aaataatcta tgtattcttg ttttttacta tttaatgtat ctaagagaat ttttttcata
    12421 tttcctccta agatttattt ttacatttaa aatttcaagt ctaatctcat ctatttaagc
    12481 catagtaaac tttttcactt agcatattcc cattcaatgt ataaagatgt atgcaaatta
    12541 aagtataaaa atatatacaa aaacttaata atttttatag tttataagct aaaatttaat
    12601 tactatttag atttagctga tttttttaat tttatattat ttatccatat agcaaataac
    12661 attaccaaac cttttataat catttgatta tatgaagata cacttaataa gtttaatcca
    12721 tttcctaata ttcctattat tccgacacct atacatgtac ctactataga accagaacca
    12781 cccatcatat tagtaccacc aagcacaacg gctgctatag catccaattc tgctccatct
    12841 ccaacagttg gtgaacctga tgccaaacgt gatgatagta ttatcccagc taatgctgtt
    12901 agtagtccag ctagtgcgta tacttttact ataactttat ttacttttac tccacataat
    12961 ctagcggcct cttcatttcc acctactgca tgtacatgac gtccaaaatt agttcttttt
    13021 aatagaaaat gtcctgctat tacaactata atcataatta taactggtat tggtattggt
    13081 ccaatatatc ctctaccgag catcttaaat gcatctgtat ttactgatat aggtcttcct
    13141 cctgtaaata caaagcttgc cccacttatt atcgttaaca tggctaaagt tacaatgaat
    13201 ggaggtagtt tttgtgttga aataattgca ccttttatga ctcctaaaat tgtccctatt
    13261 gctaatgcaa gtatcatacc tacccatata ggtgcattat aattcactac tgttgtagcc
    13321 caaaatgtac ctactaaagc aattactgct cctacactta aatctattcc tcctgtaatt
    13381 attacaaatg tcatacctac tgcaagtatt gcatttattg atatttgtct tccaacatta
    13441 attaaattat ctaaagttaa aaagttagat gaagctacac tcattattat aaaaattatt
    13501 acaagtccta cagctgcact cccttggtct ctaaatacac ttgttaattt tgtcaataat
    13561 ggagaagatt tttcttcttt atgcttagtt attgaactca ttaattttac ctccgaaagc
    13621 caattttaat acattatttt caaccatttc ttcaccttct aaaaatccac ttatatgacc
    13681 ttctctcata accattattc tgtcactcat tgatattaat tctggtattt cagaagatat
    13741 cattattata cttacaccac tctccactaa atctcccatt agattgtata tctctgcctt
    13801 agcatttaca tctatacctc tggttggttc atccataatc aatatatctg gttgtaataa
    13861 aagccatctt gctattatag ctttttgttg gtttccacca cttaaacttt gtataacttg
    13921 attcatatgt ggtgttttta tctttaaagc ttctatgtat ttttctaaaa tctctttttc
    13981 cttttttcta tctataattg atttaaactt agatatcttt tttaacccag ccatagttgt
    14041 attttctttg acactcattt gtaaaaacaa tccagtacct tttctatctt ctgttattaa
    14101 tcctagtcca ttttctattg catctgatgg agttttaaac tttacttctt cattgtttaa
    14161 atatattttt ccagaatcat atgttctaag tccaaatata gcttctacta tttcacttct
    14221 cttacttcca actagtccac ctattcccaa tatttcacct tttcttatat taaagctaat
    14281 atcattaaaa acattgttct tagttagatt ttccactcgc ataatttctt cttttatttc
    14341 tacttttctt tttgggaaaa attggtctag ctttctatct atcattaaat taactaattc
    14401 ttcctcagac gatacatctt tggtatttaa agtcgaaatc gttttcccat ctcgcattat
    14461 agttattctg tcacaaatct caaagagttc ttccatccta tgggatatat atattatact
    14521 aactccactc tcttttaaat catttataag tctaaaaagt atttcagtct cttttttact
    14581 tattgaagaa gtaggttcat ccattattaa tactttagtt tctgatgata aaactcttgc
    14641 tatttctacc atttgttttt gagctatact aagctctttg actaaactat ttggatttat
    14701 atctattccc atattatcga aaatcttttt agcttcttca ttcatttttt tgtcattggt
    14761 taagactttg ccatttattt cttttcccat aaaaatattt tgtgctactg ttaattcgtc
    14821 aaaaactgtt agttcttgat atatggcatt tatacctatc tttttagaga tatctgggga
    14881 tatattctct atttctttgc cttcaaaaat aaattttcca gaagttttcg aatgtgcccc
    14941 agttaaaatc tttatcattg ttgatttacc agcaccattt tcacccagta gtgcatgaac
    15001 ttcgccatga aataactcaa aattaacatt atctaatgct ctgacaccag gaaattcttt
    15061 agcaatatta cttaacttca gaattatatt actcataatg acactccttt ttaaatagga
    15121 tttctttcaa ataatctcat tttattttac taattcttaa agtcttttat attttcctta
    15181 tcataaagta aagctggtac tggtatattc ttttctacct tttccccttt tatagctttt
    15241 actactgctt ctacagctaa acgtcccatc tctttaggtt gttgttgaat catagcaaga
    15301 atagttccat ctgttattcc acttgatgct tcttcagtta agtcaaatcc tacaatctta
    15361 acatccttat tttttgcaga ttcaagtgca gctttagctc ctagtacact attttcagct
    15421 gttgcatata ttatatctac atctggatta gattgaatta agttttctac tgtattaaga
    15481 gattcttctc tgtcatatcc tggttgtgaa tttaatatct taacatttgc tgaacctttt
    15541 aatgcatctt taaatccatc tattctctgc atttgtattt gagattttaa atctgtcact
    15601 attgctattt ctgactttcc acctaacttt tcatctatgt attttttagt gtattcccct
    15661 aattttttac cagcatcata actaactgtt cctacataac ttgtaatatt ttcagtattt
    15721 aatacagcat ctactgtaac tactggcatt ttttcaaatg ctccttctac tgctggtact
    15781 atagccgaat tatcagttcc aagtactatc attccatcta cattctgttg tataaaatct
    15841 tctattgctg atgtttgctt agctacatct tggtctgggt catttactat aactttcaca
    15901 cctaattctt tagctttttc ttttattccc tcatcaatat ctataaaaaa ttgatgttga
    15961 agagttattg tagatactcc aatagttaaa tctttcttgt ctgaaccacc tttattatct
    16021 gatttacttg gtccttccat tgaacaccct gttaacattc ctattgctag tattccagca
    16081 attcctaaag ataaaatttt tctcactctc ataattaacc tccattttta atagttttaa
    16141 atttgaacaa attagatttt tatttattat ttaaatactt ttcaacatct tctttatagg
    16201 gcataccaga ttgtgcacct agtttagtta cagaaagacc tgatactact actgcatatt
    16261 ctactgctgt atataaattt ttcccttctg ttatagccac tgccaatgct ccattaaatg
    16321 tatctcctgc tcctgtggta tcttgaacat caacacttat acaagataca tgcttgattt
    16381 cctcaccatc ataaaatcta gctccatttt ttccttctgt tattacaagt ttatttggat
    16441 atttttttaa tacttcttct atcccttcat ttgtgtcaaa aactatctta tattcatgtt
    16501 catttggtgt taagtaagtt actttttcta ttatatcttc atttagtttt actgctggtg
    16561 ctggatttaa taaaacccta atcctatttt caaaacagaa attcactaca taatttattg
    16621 tttttaatgg aatctctaat tgtagcaata ctatatctgc attctttatc tcttcttcat
    16681 tctttttaat taattctata tctactagct cattagtccc tggtacaaca acaatactat
    16741 tatcattttc acataatgtt ataacagcaa caccagaact tttgtgatta tttctggata
    16801 ctaagctata atcaacttta ttcgttctaa aatttcttat taactcctct cctaagccat
    16861 cttcccctat gcatgatatc atttttacat ttgctcctag tttagaagct gagatagctt
    16921 gattagcccc ttttcctcca ggtgatgtaa aaaaactatt acctaatact gtttctcctt
    16981 tttctggtct tttatctaca gaacatacca tgtccatgtt tacacttcct atgacaacta
    17041 tatttcccat tacattactc ctttacatat ctatttatgt ctctcgtgct gttattcact
    17101 ttcttttgtt gaatctctta tgaccaattc tggtttgaaa atcaattcat caaacgattc
    17161 ttttttatct cctcttaaac aatttatcaa tatttcactg gctttatatc ctatatcata
    17221 agacggctgt tttactgtgg taagagaagg gcttattaga cttgatgttt gaatatcatc
    17281 aaatcccacg acacttatat catttggtat acttaatcct ctttctttaa gacctcttat
    17341 gacacctatt gcaatcaaat catttgcaca aaatattgca tcaaaatcta tcttttctaa
    17401 attttttata aattcttgtc cccattcaat cttatatttt ccttcaaata cataagcagg
    17461 attgtagctt aaattacact cgattagagc ctttttatat ccttctaatc tatctactgc
    17521 tatttcattt tgaagtggac cacttaggta tattattttc ttatttccaa tatctaataa
    17581 atgttttgta gctatatagg ccccttcaaa gttatttatt ttaacaattc cttttaaggt
    17641 ttctttgttc atacttatct tcttatcaat caagacagtt ggtattttta tatctttata
    17701 atttgcttct ttccagtttg tatttgaaga tgctgcaaat ataattccat ctatcatcct
    17761 ctctgatagt gttcttattc cttcatattc ttttttagca tcttcatctg tattacataa
    17821 aattatattg taaccttctt cattagcttt gtcttctaca cctcttgata catctgtaaa
    17881 gaatggattt cgtatatctg gtataagaag ccctatagta ttagtttttt tagttactaa
    17941 actttgtgct aatttatttg gtacataatt tttttccttc attattttca aaattttttc
    18001 tcttgttgcc tgacttatat tttcatcttt attattaatg acttttgaca cagttgtttt
    18061 agaaacacca gccaattcgc caatttcttt tatagttata ctcaagaagt tcacccccaa
    18121 aatttaggaa accgttttac ttaaccggtt acctagatac tatcacatct tttttccatt
    18181 tgcaaccatt ttatataaat tttgatattt ttcccttttt tcaaaatatt gcaacttata
    18241 attcaatttt aatatttaaa ttaatctgca aaaaaaaatt aatatacttt tataattatc
    18301 taaaagtata ttaattaatt taagcagtat ttattaaagt tcaaatcctt gctctataat
    18361 cattttttta tcattttcaa tcccacttcc aacagttgtc aaataatcac caacaattgc
    18421 agaatttact cccccattaa atccaacttt gtcatatccc tttaatgata gccttccacc
    18481 agcatacctt atgtacacat taggaattat aaacctaaat actgctgttg tttttagtat
    18541 ttccattggt tctaatacat cataattttc cattggtgta ttcttaatag gatttaaaat
    18601 gtttattgga aatgatttta catttaattc tcgtatttca aaagccattt ttagtctctg
    18661 ttcttcactc tcattcattc caattatacc acctacacat acatctagcc cagctttttt
    18721 agcatctttt atagttttta tcctatcttt atatgtgtgt gttgtacata tattatgaaa
    18781 attatctcta cacgtctcaa cattgtggtg atatgtagtc acacctgctg acttcaacat
    18841 tttagcttgc tcataatcta taatacctaa agatgcacat aattttaaat tagtattttc
    18901 cctcaatcct tcatagatat ttaaaatatt attaaactcc tttcctgaca tacctttacc
    18961 acttgtaacc aatgagaatc tatgcactcc tttactctcc atctctttag ctctatttaa
    19021 gatttcattg taatctaata atgaatattc ttctacacca gttttataat gagatgattg
    19081 agcacaaaat ttacagtctt cactacattt gccagattta gcattcatta tcgtacataa
    19141 atctgctttt cttcccataa aatattctct tatttcattt gcagatttta atagaatatc
    19201 aaaatcgttt ttattatttg tatcaaggct tattaaatta agagcttctt cataacttat
    19261 ttctttttct ctcaatactt tattttttaa ttttattatg tatttttcca tatatcctcc
    19321 tatttgtcaa cttaatattt ttatgtagtt tacttattat atatagtatt gcatatataa
    19381 catttattgg tcaactttat tttagacaca agtttactat acacaaaaaa atactgcttc
    19441 aaaataatat tagtaaatgg attatacata ttattattaa aacagtattt tttattagaa
    19501 tataattctt actggtttat aaaactatcc ttgtcttaac tccatccaag ctttccattg
    19561 taataacctc taagccttca tattctctaa atagccattt taaatccttc atggcttgat
    19621 taacctgact ttttgttaga ttatttataa aatattcttt tctagtttcc tcatactctt
    19681 tgctagtcaa aaagtttctt agaaaattaa acgcaaatac acttggaata ttgtttacat
    19741 tagccttctt gctgttttcc ttctcatagt aaattttcat aaatgcatac tcctttgata
    19801 cttttagatt tctcttacaa taattatatg catttaagtg ctactattct tataatttta
    19861 ttaattcata ttctttactt cccataccca tttcacatgc atagtctatt attctagttc
    19921 catttacatg aggatgttca tgtttgaaaa catcatgacc catttcctta caaatcaaat
    19981 catagcatgc ttgttcaata gctacagggt ctgttgatgc taatattcct atatcatggg
    20041 caattggtct acctgaccaa ggtacacaat cacaaagtgg agttacattc ataacaaaag
    20101 ttatataccc taccttgtct tttttatttg atactgctcc ataagcgtat tctgccatct
    20161 tttcgacaaa cacatctgag tcactttccc attgtatagt tactgctcta gttggacaga
    20221 cagttggaca ttctccacat ccataacata catcagaatc tataactgct tttttatcca
    20281 ctatacttat agcttttgta ggacaagaat taacacactt tccacaacct acacacttct
    20341 tttcctttac tactggagta acatcagaat gttgcatttg ttttcctgct gcacttgcac
    20401 atcccatagc taagtttttt aatgctcctc caaaacctgc tgcttcatga cctttaaaat
    20461 gactcattac tatcattgca gaagaattat aaatttctcc acctattttt acactttcaa
    20521 aatgtttttt gtctattttt acattctcat aatttctact atataatcca tctgcaatta
    20581 ttactggtgc atttacaact gcataagcaa atccattttc tatagcagtt gtaagatggt
    20641 ctacagaatt tgttctactc cctgtataca gtgtatttgt atctgttaaa aatggctttc
    20701 cttcgcagtc tttaacctta tcaaccactt gtctaactgc cacaggactc atatatgtag
    20761 tatttccctt ttctccaaag tgaagtttaa ttgcaacttg gtcattcttt tctattaaat
    20821 ctttgaatcc tgcttcatca aataatcttc ttacattgtt tggtacgttt ttattttgcg
    20881 aatttgagta taaatcacaa aaatatactt ttgacttttc catatctaca cccctttaaa
    20941 atatttactt atactaattt tatcactttt aaagtaattt ttagtatatt ttaacataat
    21001 taaatattct tatatttatt tgatacaaat aatattattt gtattatttt ctacacttaa
    21061 ttctatatat taaaaatcaa aaagacttga aacttaagtc caagtctttt tgatttttag
    21121 gtgtagtttt taacaacata agattacttt accttgtgat aaaggtatta ttaaactggt
    21181 tttttattaa ataaaataac agctgctagt aaacatacta cagaaataag aactgttata
    21241 ataattggaa taataaattc tggcattgcc actttccctg aaatcaagtc tatatttttt
    21301 gacatcaaga aattaggagt ataaggtgca atctgtttag gaatagaaat taaactcaat
    21361 aaaagagttg ccaccaatac aagtaaaaca ctggcaaatg ctggtctaaa tagtacacac
    21421 ccaagaatta atacactaat atacataatt cctataagcc ataaattaaa tgctgaaaat
    21481 ataatatgat taagatttgc agttggccaa aaatatattg tatagccata agcaaataaa
    21541 aaactcaacc aaaaaccaat tgtcataata atcaccgtaa cagagaattt agacaatact
    21601 atagatgaac gtgataaacc tttagtcagc ataataacaa gtgtcccttt agaatattca
    21661 tttgataaaa tattacaaaa aataattaga gttaaactca taccaagacc agagatattg
    21721 ctaaaaaatt gtgtccaaga atctaaagca gttggtgcag cagtaacttt taaagtcggt
    21781 gcaaaatgag caatcaagtc tggcataaat tttgcagaaa taggactact tataccaaat
    21841 attagaaata aaataattaa actaaataat ttataatttc ttaaattctc tttaaattct
    21901 ttttttgtaa atgctatgta ttctctcact ttactacctc caaaaataaa ttttcaagtg
    21961 ataattctat tagttccatc tttactggac aaagattcat ttctgcgagt gtagatataa
    22021 ctgttttttg tattgctttt atatctttac catgcaaaac aatttccata ttagtttctt
    22081 ctgaatcttt tagtaaggat ttaataccat cactagactt aaacttttga atttcatcat
    22141 tacttgcaaa ctccagtaaa agtctatcct ttccgtgcat actttttatt tctgaaagtg
    22201 taccactaag ggcaatgctt cctttgttta atatagcaac atggtcacag attctttcaa
    22261 catctgatag tatatgtgtt gaaaatatta cagttgttga gtctttgatt ttaagcataa
    22321 tatctaatat ttcttttcga ccaacagggt caagtgcact tgttggttca tcacaaataa
    22381 gtaatttggg gcgtgaaagc aaagcttgag ctataccaag tcgttgcttc attccacgag
    22441 aaaacccacc tattcgtctc ttttcatttc caagacccac aagtgataac aactcttcac
    22501 tccttatctg tatttctttt tttgaaagtc ccgtaatttc accacaaaga gaaagatatt
    22561 caagtggtct catatagttg taaaactctg gaacatcagg cagataacct acatgacgat
    22621 tagaacttgt tttcccatag gtcactttct caccacaaac atctatagtt ccactatctg
    22681 gtttaagtaa cccaagtacc attttcattg ttgtagtctt acctgcacca ttcttaccta
    22741 caaacccaaa gactgaccct tctggtacag taaaatttaa attatctatt atctttcgat
    22801 taccaaaacc ttttgataat ccatttattg agacaatatt catcattctt cacctcttcc
    22861 tattgtaaaa tacaaaattg gtcctattag actaataaac agtactacaa taatccatat
    22921 tgccttattt ccaattttat aatttggatg tctcaataca tgaacaagtg cagtaattat
    22981 aagtattaaa tctaaaataa taacaggaac caaaagtggt aagtattcca tcaaattttc
    23041 cataatctta ttcctccaat aaagataatt tttctataag aaatttgtat tctttgtttt
    23101 cacttaatac agaaaacatt ggattatttt taatagcagc aacaatacct gcttttacga
    23161 ttgtatcatc acgtgtaata cctgttccaa tttcagaaat ccatctgtca agtttattaa
    23221 aaaagtcatc cccatgtatt ataattgggt atatatcacg tgttgctaaa tctacatatt
    23281 ttcgtaatga tttaatagct ccttcattat caccatgtat taaattaatt tgtgcctgtg
    23341 ttaaatatga agaaagcata atagcaggag ataatgtatc aagctcaaat atatctgaaa
    23401 ttgctagaat acgtctttct atttccttca tttgtaattt atctgcattt tgcaaaatac
    23461 ccactaggtt ttggactaat gatataaggt tctgatatgc accaagttga aatgtttctc
    23521 tcgcttcatc catttgacca ttcatcattt gcccctgtgc aagaagtata gattcattta
    23581 tcatgggaaa attgctgttt tgcaataaat caatcacttg tatgggttga tttaacaata
    23641 tataacaagt tgcctccata ctttttgctt gacggcaaat atctatgtca ttacaagttt
    23701 cctgtatacg aataaatatt tctaatgctt cactaattaa tagttcacgc tttttttcat
    23761 ctacaatatc atagtgatta actattaaaa gacccatccg aaaaatcaaa ggaaaacatg
    23821 aataatattt ttttatgatt tctcttattt catccatgac ttcatcaaat ggtttaaccg
    23881 taaagtcttt gcacagttta ttatacagtt tattaatatc ttccttcatc atttgaggtt
    23941 cataacatat taactcatca acggtaatgt tgaaatatgt cgcgagttgc ggtaaaaaag
    24001 ttatgtcagg atagctttgt ccagtttccc atttagatac agaagcttta gaaacgccta
    24061 tataatttgc aagttcatct tgtgtaattc ctttttcttt acgctttaaa atcagcgttt
    24121 ttgcgatgtt taattcattc atgtcaatcc ctccttatgt ataattatat ccttaaacat
    24181 gtaaagctaa caagcgattt gaagttgact tttgacgaaa aaatcaacca cagagaaaca
    24241 aataaacaag tatataaaat atatatttaa atttatcttt aatctctttt gtaaaatatg
    24301 taaactttgt aaataaaatc catattatat aaaactaata gttaatctat ctaaatacag
    24361 tttttattta tcacgttttt aaatattttg tgtaaaacct cttttaatat aaacacctta
    24421 ttttcatttc tcatttcata ttctttttaa aatactgaat ataactcttc gattttccat
    24481 ttaattcttt tataaagtta ttattcgtat ttatttctct aattataaca ggcaactatc
    24541 atgtattggt atttgtctat tatataaaaa tctataaaag aaaagcagtc aatcatttag
    24601 attaactgct tgtacatata tcttattaaa ctgtataaac tataattttt attattctcc
    24661 attttcatcc caagtcataa atccccatcc actatctgga taagtatcaa tagacttttc
    24721 tctaatcata taaccaactt tataacactc ttttgttatt ttattagata gtatacatcc
    24781 ttctccattt atctaattcc attcaattaa cttttctttt caaccttaac ttttataaaa
    24841 tcatcttttt taataatttc atacttattt tctcttcaac atcatcaaat gagggtgtct
    24901 caaaattaac tttttacttc ataaggcact cttttttgta tgaagtaaaa tatattttca
    24961 tcatttcaac aataagtaag aggcttatct ctattttgag acagccccat ttgatggtgt
    25021 tgaacattta aataaggttg aactcgtttt atctctcttt ttaagatatc tgtaaatttg
    25081 cacataaagt ctcttcttct tttacaattc taatagtttt attaaagcca aagtatatat
    25141 ctccatattc tttcgtatat gatttattgg tactctttca tctactccat gaccaccagt
    25201 tttatacgct aagaacttag gtccaaacgc caccatatct ttaaaccaac ttgcatatgt
    25261 gccaccaccc atagcaattg tatatccatt ttcatctgtt acttcttcat aggcttctaa
    25321 taatttttta agtactaaat tactctcatc cacataatgt aagtctttat gagcaataac
    25381 ctctattttc caaccattac aaaaaacctt tttgaatcta tctattatat tgtttaaaac
    25441 tatattttta ccatacctta tatctaactc acatacgatt ttttcctcat ttacttttaa
    25501 tattcctaaa tttaaggtag tgtttttgaa tacctcatca tattgattaa tccctaatgc
    25561 atctccataa taatcactac aaaggtattt tgaaactaat tcgatatctt ctctatgctg
    25621 aaaatccata ttatcctcaa ctatctttat taccatttta gatatagcat tttctccctt
    25681 ttcaggatta cttgcatgta cagctactcc tttagtagtt aactctgaaa taattccatt
    25741 aataattttt gcagtacaat gagatggaac aacattttct ttagttcctc cccttactat
    25801 tatgtaaggt ttacttttgt caattttaat ttctttttct atccttatat gaacccttcc
    25861 tttttctcca ttaaccactg gatacatagc atctggagta aatcccatac ttggaatttc
    25921 ttcaccttta tctaagtaat atttgataca tttcatacct gtctcctcat ttgtaccaaa
    25981 aattaatctt attcttttat taattgtagg atacatatcc tctatgtttt tcaatacatg
    26041 tataagcata gatacagcac ccttattatc tattacacct cttccataaa taatattgtc
    26101 aattactgct cccgaaaatg ggtctacact ccacccctct cctggtggaa ctatatctaa
    26161 atgcattgga atccctatta attcctctcc ctctccaatt tctgcatatc caatataatt
    26221 atcaatattt ttaacactaa aacctaaact agcacagtaa tctaaagttt tatctaaaga
    26281 ttttttaggt cctataccaa aaggacattg tgaaataggt tcaccttgtt gtgaatcaat
    26341 ttttatcaat tcttttagaa attctatggt tctatttaaa ctattatcta cacttctgct
    26401 tatatccata aatcaaccta ctttctatct ttttaaagcc tgtccttaaa gaacaggctt
    26461 ttagcattaa aaattatttt tttgtaaata ctaattcaca aacttcatca tcatgagcac
    26521 atttttgagc aaaatctaat ttaacacctt gtgcgcagtc aactcttcca taatccccat
    26581 aacaagctaa tctacaaatt tcttttcttt cttctttagt taatccatat tctttccaag
    26641 cagcatctaa tggacagtga tggaatttta aaacgccatt ctcatctgta ttttctacta
    26701 attccatttc aaacactttt tgtcctttac tgttatataa catttctgcc atttttcctg
    26761 gagtatctgc tacgttatat ttttgacctc tcattttacc aaatccatat attgcttctt
    26821 tacatatatc atctggatta gctcctaatt tttcagcttc tttcataaga agatagaacc
    26881 atactgctct ttctcctgtt gctcctctaa ttccttctaa atattccttt tctgtataca
    26941 ttttttctga catttctttc tcctcctaaa attaaattct atattttaat cactgtgaat
    27001 tagaatattc caatgaatga ccctaccaat gctaatacaa ctaaaattac taataactta
    27061 gttattgtcc acttcttgta tttcattccc caatatacga agaatacaac accaagtggt
    27121 aataaacctg gagcaatcat atttaatgta tcttgaacaa caattgcttt accaccaata
    27181 tcaaatgata gcggagttga aagcttaact gtagttgcag ataatgctcc catcataaac
    27241 attcctagaa ttgaagcacc atcaattatc ttattgacaa taccacctga tagaattttc
    27301 ttaattgaat ctctacctaa agaatatcct aaatgaaata agtaataacc ttcgcaaaat
    27361 agtaatatac taaacattat tgggaaaaat gcacctataa cacttccttc tgctccaaat
    27421 gaagcaccta aagctaagaa tattgtttga aatgtaccaa aatctaaagt atctccaatt
    27481 ccagccaaag gtcccataag accattctta acacctgaaa taacttctcc tgggattttt
    27541 ccttcagtag ctttctcttc ttccatagct aaaactgtac cgtgaattaa tcctccccat
    27601 atagcttgag tattaaaaaa ttgtaagtgt ctctttaacg catctactaa atcttctttt
    27661 ctcttatata acttttctaa agccggagtg aaacttgcac aaactgctaa tgcttgcatt
    27721 ctttcaaaag agttggataa ttctgctgtc caccaccatc ttagatatgt ttttgttaca
    27781 tctttctttg taagtactct tgtcttatta acttcagttg gaccttgtcc atttttaact
    27841 tctttatttt ttatttcttg cattatactt cacctaccct tttgtcttct ttcataaagg
    27901 taattaaaag agctatacaa acaccaaata ttgcagctgc agtaattgat atttcaaaat
    27961 attgaaccat aaaaaatcct ataataaaca ttggtaaaaa tttattttta cctatagtaa
    28021 atattgttgt tgcaaaacct aatgcaggta atataccgcc cattactgtt aatccattca
    28081 ttaaccattg tggtataaca tctaataatg attgaactaa atctgctcca aaaaagttac
    28141 atacaaaaac tattggaaac cttaaaacaa acccaaatat taaagaataa agtgttgcac
    28201 atctccagat gccttttgta ttaccttctt cagcatactt atctgcctta tgaaccaata
    28261 cagcattacc agttcttctt aagttattaa ctaatactcc tataacacct aaaggtaccg
    28321 caatagatac tgcaacttct gcattaacac cagtttgaag tgctactggt attgcaataa
    28381 gagcagctag acatttatct gatggaatat ttccccctgc tgcgaccatt cctagataaa
    28441 ccatttctat acttgcacca acaatcatac cagttgttac atcaccccaa agtaatccaa
    28501 ggctaaaacc tataaataaa ggagacttta gtgttgagaa aagggtatac cctgcaagac
    28561 ctgctgcaaa ccaataccat aatcctgtta caattgccaa catcaaaaca gattccattt
    28621 ataattcctc ctaatttata ttttttattt aaatttcttt aacgcatttt catatgtcat
    28681 agatgattcc tctggtataa cttgtaggac tatttcaacc ccattttcat taagttcatt
    28741 aagttgttct acatcctcat ttcctaatga aacagctgta aacaccattt tctttcctgc
    28801 ttcatttgga atacctccta attgaatctt ttcatattta agtccagctt tataagcctt
    28861 atttgccata tcaacatttt taaatagaat aaatatattt ttatctccta aagtgttgtt
    28921 ttggaatgct tgtcccgcat cttctgctgt taaaataact acttctactc ctgatggtgc
    28981 tgccatttta tatatgtcag ccataaattc atcttgacct aagaagtcat caactattat
    29041 tattttttga gcttgagcga tttttaccca ttttgttata acttgcccat gaattaatct
    29101 gtggtctact cttactaatg ttatttttgc cattatattc acctcataat ttatattttt
    29161 ttaaattctt taattaaatc cacacaaagg ttattatttt ttttgacgat tctttctggt
    29221 aatgtatgta atttatcttc tttgcttaat tcttcatcta cgattaaaat tgtctgtaag
    29281 ctaagtccat ttattgcatc tattcctctt cttatagcaa cagctattgc aacacttgat
    29341 gtagatcctc catataaatc tgatattata attttcacat cttctttatc tactacttct
    29401 tcaacctctt tcataaatac agaaacatct ttttcagaac ttagtgataa aacttcatat
    29461 tgatttgaca atccaaagtt tttatttgtt tcttctacta agacttttcc ccaagttcca
    29521 tggcacagta tgattacctt atatttattc attgctaaat aaactctcct ttatagcgat
    29581 taatattttg gtttaattct acttcttctt catcaggtac catttgaatt gatacattaa
    29641 tattcttttt aataaattca tctattaaaa gaaactcttt tttattaatg tatacatttt
    29701 gagatatttt cttttttcct attcctgtag gtattcttcc aacaataagt tttttgatat
    29761 taatcccttc ttctatcatt ctatttgcag tatctaaatt tttaattaat acaaaaacgt
    29821 tatcatcagc ttgattatta taaaaatatc tccttgattc acttacagtt aatattgata
    29881 aatcaatatt ctttggtgct gccattttta tcaaaccttt agcaataaca tcattggttg
    29941 tttcatcatc tactactact atctttttta cacctagatg cttaacccat ttatatataa
    30001 cttgaccatg tattaatcta tcatcacatc tgcaatattt tactaaagac atttttaatc
    30061 aaactccttt ttctattaca ttgagctaag aactttgtta atattgttta taccagtttg
    30121 tcctgcttca atacatgctt ctgctaactc ttctaaactt tttccctctc tattaattaa
    30181 agcttcaagt agcattggaa gatttactcc agtaagtgca tgaaacttgc cacctttttt
    30241 aaggtttaaa cttgcaacat tacttggcga acctcctaat aggtcagcta atactaaaac
    30301 tccgtcacct tcatctaatt catttaactt ttctacaaaa tttttattta agtcttcaat
    30361 attatcattc ctatttagtt taattgctgc tgttctttct gctggtcctg caataagttc
    30421 acagcttttt ataatttctt cactaaaact cccatgagtc ataactatta ttcctatcac
    30481 caatttcacc tcacttttct tgtcactttt tatataagca attgccgtgc caacttatgt
    30541 gtatcaataa caaatggcta ttttacagga tttaatcaaa actttttatt caattttttt
    30601 taaaaactta ttatttcgat atgtatcaaa agattttttc aacctgtatc aaatctgtat
    30661 caaaaaaagt aaaaaaaaga tagccttttg gctattttta aattttgtat caacttttga
    30721 atttatctgt atcatattgt gattcaaata tatctaatat atttgcatat tccatatctg
    30781 aaataattat atcgaatgtc tcttctacta gcttaaaact ttcttttata actaaatata
    30841 attctttatt tctactaatt ctttcttcat atgatttata aactaaagac tcttttatta
    30901 ttactctttc aatcatacat gaacaatgaa atataaactt tactaaaagt tcatcttgaa
    30961 ttactaaaga atagtgttca ctaattctat taattacttc ttttaatata aaataagcct
    31021 tttccccatt taaaaaagtc aaagttttac taatattatc aattagtaat ctatcaaaat
    31081 atctaccacc attatctttt tcagcatatg taacttccat atctccataa tgtttcttta
    31141 tagattctat aatattaaaa tataatttct ctaatgtcat ttctggagtt agagcctttc
    31201 tagttgcttc tattacaata ggtgtactta ccatatctat tgcttttact tcttttcctg
    31261 tagcatcacg aataacttta gcaaatgtta aaatagaccc catatcagtt aaaattaaaa
    31321 ttccctttcc tttatcaatt ttattggcta tttctacagc ttgtactaaa atatcctgaa
    31381 ctctatcttc taatcccata ttaattgcca ctgcatggtt acaatccagt aaagtattgg
    31441 ctacattcac catgctagat gctgtagatt caccatgcat tatgactaat attgctatac
    31501 tctcctcatt taatcccaca tacgataaat aaagaaatgt tgctaaaaaa attgcttcat
    31561 cttctggaaa ctctactcca tacattaatg acatgttaga taatatttct tttgctatgt
    31621 tatattcata tgctctttcc ttcataattt catctttatt aggatgcttt actatatttc
    31681 caatctttat tctttcaatt aaattctgaa tatgtaaagc tatagctttt attaatttat
    31741 ctaatccttc tcttttgctc catttatcat gcttttgtaa aatatatttt acagtagttg
    31801 caattgaatc attaacaatg ttgttataag ctgtttgatt actactatta tatataaaag
    31861 tattcattaa attataggaa tacttctgta tgtctccatc tataacactt ctaatttgtg
    31921 actctttaat accttcttgt tgaagtttat tccaagtttc ttttatcatt ccataaaaat
    31981 ctaaatcata ttttttattt tctattaata aattttcact ttcaaaatcc ttttcttctg
    32041 gtaaaaaaat aatatcatct tttccaataa aactgaaatt ttgtacaaac tcctcttttc
    32101 tattatttcc atataatgat tctctaatat tatttggtag aagagagagc cttacatata
    32161 cacattccct tttataggtc atataatcta aaaaagctct tgcacaaatt aattgtatat
    32221 cgctttttaa ttggcctatg tttcccttac acttatataa aataaatgcc ttaataactt
    32281 cttttgaaac ctttaactta acaccaatcc taacactctc ttctttaaaa aaataacata
    32341 taagcttcat tctctcttga aagcctcttt cctctaaaga cggaatcttt atagttacag
    32401 gtattcttct taaaaacgtt tccaacattg ctgtttctgg gtcttctgta gtggctgcaa
    32461 ttatcataac tctacactcg tgtaccttat tagcctctcc taaccttcta tatatccctt
    32521 tatccataag cagaaatagc atttcttgtc cctctgcaga taatctatga acttcatcta
    32581 aaaacaatac tccattatta gcttcatgta caataccata cttatcttta tcagctccag
    32641 taaatgctcc ttttacatat ccaaaaagct gcgctaataa taattgctga ttatcagaat
    32701 aatcagcaca gttaaatatg ataaatggtg cattatcact atcttctttt atagttaaac
    32761 tatacttata catatattct gcaaatatag atttaccaac tcctgtaggt cctattatta
    32821 atgtactcaa ccccttaggt ggatatatta ctgctgcttt agcctgttct atttgggatc
    32881 ttaaacttcc atcacatcct actaaattaa aaatattgtt ctcttttttt atactctcta
    32941 aaatatactc atctttgata gaatcatttt cactatattc gtttatagag ttaatattat
    33001 caaatatccc tttatttagt ttctttccta aaatactctc aatactttct ttatataagt
    33061 actgaacagg cttacccttt atctttataa tgatactttc ttttattaat tgatttaatt
    33121 ccttactagc attacttcta tttataccta cttcagtagc tatatattct gcatcaaatc
    33181 caaatctaat gccattatta aagttcaagt gttcttctgt taaccttttc aagcaaatta
    33241 aaactttttc tttccttgat agcttctttt gtgtcatagt taacctcccc tataagcagt
    33301 aatactgtta aacatttatt attaagctta attctacaat attttaatat ttttgtctaa
    33361 ttatgtaact attgattttc tttcctattc gttttataat ataaattacg ttaccctcat
    33421 cttttataga ggaggaattt atatgaattt tttatcagat ttacacaccc actctatagt
    33481 cagtggtcat ggctatagca ctttactaga aaatattaat tattgtaaag aaaatggaat
    33541 taagatacta ggtacatctg aacatggtcc taagatgcct ggttctcccc acagatggta
    33601 ctttcacaac attcaaaata tcccaagaat cataaataac attataattt taagaggctg
    33661 tgaagcaaat atactagata ttaaaggaaa catagaccta gagccttttg taattcctcg
    33721 gttagattat cttatcttgt catttcacga agctgtgttt tctccaaata cattagagaa
    33781 taatactaaa gctttaataa atgcaataaa taagcatgat aatattgaaa tattaggaca
    33841 ccttggaaac ccaaactacc caatcgacta tgaattaatc attaaattag ctatagaaaa
    33901 gaatatctta atagaaataa ataattgttc tattaaaggc gtgtcaagaa atggtagctc
    33961 ggataactgt aagtatattg ctacactttg caaaaaatat ggtgctaaaa tcatactaac
    34021 ttcagatgct cacatttgtt ttgacatagg taactatgag tattctgaaa atatcttaaa
    34081 ggaaattaat tttccagatg aattaataat gaattatcct aaaaaactta ttaatcattt
    34141 tcatcaaaaa ggtaaattac tagatgttga ttatacaaat atctaagtaa taatacatgc
    34201 ttactttaac tcccataaaa attttaatac tatttatatt atatgaaata aagaaattat
    34261 aataaagaag ctatttaata aagcttaatt acttttatga acttgatgga ttgatatggt
    34321 ctatgatagc aatagaaatt ataacatttt taataagctt agttttatat accttatagt
    34381 ataaagctaa aaaaaagaat catctaaaat aaagtaaatc ttatataagg tttactttat
    34441 ttttatttgt aataatcatc aatacaaatt attttattat atgtaattat ttatgtctta
    34501 accaatttta tcgaaaataa cagtcttctc ttttaaagag atttttccta cttgatatcc
    34561 atattcattt aattcctttt tatatttcaa aaacgaatgg tctatctcta tcccagcaat
    34621 atcttttatt tcttcaaaag ttaatttaaa cgattgacta ttgtttttct taacatattc
    34681 ccataaagaa ctatatttac tcatcttata tcactccttt taaaatcata tcttatctat
    34741 taatttatat tctatcatta ttaatgtata aagtcatgct acttattatt tatatataaa
    34801 aatatccaaa taataagtag caatttttt
//
LOCUS       DAMJYR010000032        33069 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.032, whole genome shotgun sequence.
ACCESSION   DAMJYR010000032 DAMJYR010000000
VERSION     DAMJYR010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 33069)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33069)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33069
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.032"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            <1..723
                     /locus_tag="PC471_001493"
     CDS             <1..723
                     /locus_tag="PC471_001493"
                     /inference="COORDINATES: protein
                     motif:HMM:NF012301.1,HMM:NF016296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2788628.1"
                     /translation="SDLMIITICENDDMQRDIIYDFVKIFLTKIKVKHYDILMFKNGE
                     ELLEEYPNKVDLILMDINLGGRNGFDISKDIRKFDKDARIIFVTVSEKYAIKCYEINA
                     FRYMIKPIYYKEFEKYMLDFAIDWRKREAIITIKRKKQIIKIEEHNILFVEIVNRKLR
                     VVTKNEEHIINDKMDMFEEKLKEDIFFRCHKSYLINLLEVTNFNYNFVYLTSEKIKIK
                     VAPKKTKKLKEKLANFFREIIW"
     gene            complement(1187..1540)
                     /locus_tag="PC471_001494"
     CDS             complement(1187..1540)
                     /locus_tag="PC471_001494"
                     /inference="COORDINATES: protein motif:HMM:NF024252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2788629.1"
                     /translation="MNENLKELRIKLGLSQKEFAEKLLVSDATISRLEKGERKLTDRF
                     IFQICEVFNVNEDWLRNGNGEMFKNIDDIELAAMMGKTFSSNDEFLKKVFLTFAELTD
                     DEREVIQKVIDKLSK"
     gene            1720..1890
                     /locus_tag="PC471_001495"
     CDS             1720..1890
                     /locus_tag="PC471_001495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2788630.1"
                     /translation="MYNNNDKKLINLFESFKKIDYTEQSLVYFLSLSFIVNRKCHKDA
                     NLYSRRGECNYE"
     gene            1883..2689
                     /locus_tag="PC471_001496"
     CDS             1883..2689
                     /locus_tag="PC471_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor KilAC domain-containing
                     protein"
                     /protein_id="HDF2788631.1"
                     /translation="MNNLNIKGKQKFMDLEIPIIEGGFGEGKKCITDKTISEIHNMEI
                     KHVRELINRNIRRFINAIDFIDLKVVVDNDYNLEVLELLSYRKMQISKANNIYLLSER
                     GYSKLIKIMDTDLAWEIHDKIMDEYFNMREQLIDSYMIQDSIERAKRWIEEEKERLLL
                     KETIQVQQPKVEYHDKVLNPDKLVTTTDIAKDLGMTAQALNKLLHLNRIIYPERINGR
                     IKCWKPYKNYLWLIPEYADFKITEHDTQLKWTEIGRKWLIEFVENIKKIS"
     gene            2753..2977
                     /locus_tag="PC471_001497"
     CDS             2753..2977
                     /locus_tag="PC471_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2788632.1"
                     /translation="MIKQILNKRGSLLNLASEYLTEQDYLKFQGWYTHRYSKLDAYDA
                     SINEYLNYKNDKKLITYVFNLKFQEALIEI"
     gene            3001..3540
                     /locus_tag="PC471_001498"
     CDS             3001..3540
                     /locus_tag="PC471_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2788633.1"
                     /translation="MNYNYVTKIDLKYGNTQFGFEKYSDALDKYRELKEEFKYVPCII
                     KLHSYVAHQDLYVKEIKADGFEKKYLDIKNLIKLFKEHKQNISDELSLCDKKKEEVLH
                     VIEEMNLDTASLNDKIDTIKELKNTLVKRRLVKYELNKYFAFCNTLYEIEEELKKYES
                     KYKQKENQGKSRFKKIKNR"
     gene            3545..3685
                     /locus_tag="PC471_001499"
     CDS             3545..3685
                     /locus_tag="PC471_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2788634.1"
                     /translation="MEEYHFNLIKKLCAERKIHKGWFRNIEKCGCGGKCNKKNCKMLE
                     KL"
     gene            3696..3929
                     /locus_tag="PC471_001500"
     CDS             3696..3929
                     /locus_tag="PC471_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009900684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2788635.1"
                     /translation="MENQIELTEKDIENTLSALNIIKYICIKSKHCKNCPLGYMKNLD
                     EHNCMLYELIDKGLLPCDWSVKKVTRLLDTTEE"
     gene            3948..4361
                     /locus_tag="PC471_001501"
     CDS             3948..4361
                     /locus_tag="PC471_001501"
                     /inference="COORDINATES: protein motif:HMM:NF021182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2788636.1"
                     /translation="MELDFRIWNGFRMIHGEELRLLTEEMFIKSGYSEQYYNDICIPR
                     VEDLKIMQYIGLKDCFEKKIFQYDIVKFIFDGVWKVCLVNLSLPKGATVTFESIGGKY
                     TFNLSETFSKDRKLEIIGNFYENKDVIEKYNLSIE"
     gene            4371..4565
                     /locus_tag="PC471_001502"
     CDS             4371..4565
                     /locus_tag="PC471_001502"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2788637.1"
                     /translation="MDEMIIRVKMGEPLPTLGKIIKCTTCLTNEEKCFKVNKICKLSW
                     INDRNSEGLIVECKGKYIKY"
     gene            4772..5326
                     /locus_tag="PC471_001503"
     CDS             4772..5326
                     /locus_tag="PC471_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003479931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2'-phosphotransferase"
                     /protein_id="HDF2788638.1"
                     /translation="MSKKDKLSIFISLILRHKPETIGIKLDDYGYADVNELIEKINNT
                     GRNINIEILEQIVKEDNKQRYSFNDDRSKIRANQGHSINVEVELKELEPPKCLYHGTA
                     TRFLENIKKEGIIRQSRLYVHLSNDIDTAVKVGKRHGVPVVLKINTGKMYENGYKFYL
                     SENNIWLCKYIPFEYVEKISRVVK"
     gene            5353..5679
                     /locus_tag="PC471_001504"
     CDS             5353..5679
                     /locus_tag="PC471_001504"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2788639.1"
                     /translation="MDFNFRKICEKDELTDDFIIKICQLSHEEFGGTIYVPCNIHPCG
                     WDELEVPNPKDLDSDKLMEEFLLEYKFECENISSNIYDSDHPFIGKTLREILMIVDCR
                     FIIGVI"
     gene            5694..6470
                     /locus_tag="PC471_001505"
     CDS             5694..6470
                     /locus_tag="PC471_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2788640.1"
                     /translation="MENIELTPRDYFEDIKNKKQKNTNENLDIVYENSLILLNKYIVT
                     GQKKGAKKLMFLLECIEKERNVIELGIDTFVYKEDIEKYIDDISKNTVKIIELENYER
                     EIPDEIVEIIEKTKDIFDKMYVLFTDYTGKITKEISKERRDKDPILFGVFSDEKSRAI
                     VDRFYYLGDWEDEYCDLTLEKMINDYKKKGRQDILKHIITPKSLDELKKELDSLEKNN
                     DTYKQVANIKPLPTTKNSSVEPPKLNIFKKIKNRVFKNEK"
     gene            6460..6861
                     /locus_tag="PC471_001506"
     CDS             6460..6861
                     /locus_tag="PC471_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2788641.1"
                     /translation="MKSNVDLTENRDFRDVRGRGINILGIEDAPNRMLSFSDFGIGRL
                     YGKNLEFEFNKNEVFLTGNANDREEKKFLILCEENKVCVRCGDELREGWDKVSIELCN
                     ECYEMLDVEYKNKKIIRELFFRDRDVNIELL"
     gene            7050..7370
                     /locus_tag="PC471_001507"
     CDS             7050..7370
                     /locus_tag="PC471_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2788642.1"
                     /translation="MFIKSFRCIFRHKFSRIKDCQLITTRSQQYTCKIWMCKYCGQIV
                     AGSTPVYHPNMKVNEYCVKCEFSACANNYEGYCNSKDKEIEFKSICLKGNKEILQCKN
                     FDLE"
     gene            7401..7586
                     /locus_tag="PC471_001508"
     CDS             7401..7586
                     /locus_tag="PC471_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2788643.1"
                     /translation="MKINVHVKTNKIGSDCELEFEIDDDDLKDMSDEEREDYIDKIAW
                     DYVFENFIDWNWYEVED"
     gene            7629..8576
                     /locus_tag="PC471_001509"
     CDS             7629..8576
                     /locus_tag="PC471_001509"
                     /inference="COORDINATES: protein motif:HMM:NF013623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2788644.1"
                     /translation="MNKDNLETIIEGYKVFNPDWTCRNFKYEVGKTYEHDGDIEVCEA
                     GFHFCQKAIDCFNYYSFDNKNKVAKIEAIGLIKTDGDKSVTNKITIVKEIEWEELLNI
                     VNIGNNNTGYGNTGNHNTGNHNTGNHNTGYRNTGDYNTGNGNTGYRNTGDCNTGYRNE
                     GNYNAGNYNAGYCNTGYCNEGNYNTGNYNSGNCNSGDWNIGDYNSGVFCTDVPKIRMF
                     NKETDLTYEDWMNSDARNILKRNSYLTKWIHIDDMTEEEKENNPGYEINNGYLKVFIF
                     EEMCKNMWDSLTEEEKKIIIEIPNFDADIFKEITGIEVI"
     gene            8892..9332
                     /locus_tag="PC471_001510"
     CDS             8892..9332
                     /locus_tag="PC471_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1653 domain-containing protein"
                     /protein_id="HDF2788645.1"
                     /translation="MVKRIIEKNKEKIDLERKIKYPAIYKHFKGGFYTVMGISKAVTG
                     DELDNIFKNMGCINRLDVLEYRFGSRHTETRHTETRENVIIYRDNKGNLYHYKDVDNN
                     TNLVIYKTLYDGTGAYARPIEMFFSKIDKEKYPNYTQKYRFEEV"
     gene            9335..10447
                     /locus_tag="PC471_001511"
     CDS             9335..10447
                     /locus_tag="PC471_001511"
                     /inference="COORDINATES: protein motif:HMM:NF023645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidoligase family protein"
                     /protein_id="HDF2788646.1"
                     /translation="MPTFNRYRIEDLSGLQNERWRSEDSRNICLADWIPGNISHNSNP
                     TIVDQFNDELGGLANEFTQWNMGDFFAQFTPIKKVKIGDVARKYLNPHYYIPDEFNFN
                     NITNDESLYLGIELEVDNGGKSEENAKFIQEFLGEKNCYIVGDGSLLDGLEIVTHPCT
                     LQYHKKLEYKDLFEELKEKGYKSHDTSTCGYHIHINRDFFSSDLTIQDLCITKILYLI
                     EKYWKYVTVIARRRSNQYSKRFGIKDNESMFELLIKAKGSYVSKYNVVNLQHSKTIEF
                     RMFRGTLKYETFIATMEFVHNLAYICKDTSLEDIQNIEFQDILNVYQTEYLLLYFNNR
                     IENMNNTVDLEDEYKENLRRRIFIRDEWSLFHESLM"
     gene            10514..11524
                     /locus_tag="PC471_001512"
     CDS             10514..11524
                     /locus_tag="PC471_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HDF2788647.1"
                     /translation="MDIKSRLESKEYNFLREEERLKNRMILPPIVRSFAYGTNIDTPE
                     HISDLDIRGIYLNSVQEILTMNCINKPYENKQTDTVIYPLKQIITLLLNVNPNVIEIL
                     GTRKEDLFVCTEEGKLLKDNVDLFLTKRAANSFGGYAISQLRRLQNALARDNYEQKEK
                     EEHIMNSVKRQMKTFEDRYKVVSEEEIKLYIEESKRTDLEEEIFIDVNLRKYPLRDLK
                     GMWSEMNNILKDYSKLNHRNNKKDELHLNKHAMHLIRLFLMGIEILEGKGINTYREND
                     KEFLLDIRKGKYTYNEIFEMVNELDKNFKYAEKNSCLPNSPDMKKVNELVMYMTNRVL
                     NR"
     gene            11674..12024
                     /locus_tag="PC471_001513"
     CDS             11674..12024
                     /locus_tag="PC471_001513"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2788648.1"
                     /translation="MNINKLKKIKERKTKRWNIEKITNRINDHMNIQEDIEKWDKFET
                     PIISKEVDFYLLKIGFQYGRNNGRNVLVGIKDMRHISISKVNSNLLSIDIYKFIKQED
                     GKFSEFKAHEYIEK"
     gene            12078..12479
                     /locus_tag="PC471_001514"
     CDS             12078..12479
                     /locus_tag="PC471_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA ligase"
                     /protein_id="HDF2788649.1"
                     /translation="MKELLEVKKIFDSLASTSSRKEKERILEKYKNNRMFVECLQFLL
                     DSNILTGISKNKICKNLNNTSHNELENIYDMLDYLIKNNTGRNIDVKTIQVFASKDEK
                     LKDFIFNLATKSIRLGITYKTVDKIMPGLII"
     gene            12581..13084
                     /locus_tag="PC471_001515"
     CDS             12581..13084
                     /locus_tag="PC471_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2788650.1"
                     /translation="MTDKIIIASVDGEEMIFRTEMKKLEKGDSLIVSVNGVKRLALFI
                     RYISTCEEYIEDIAFQKVDEILYENTIAKEKERVKKVFKDKCMKNYKPIVNTEIDSYL
                     RECGFNCDGRQFFKSIDNTQILISRDCDKDVLTVSKGLTITKTDITNKNLITVIKIIN
                     NSLLIVR"
     gene            13123..15303
                     /locus_tag="PC471_001516"
     CDS             13123..15303
                     /locus_tag="PC471_001516"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006356698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin-dependent
                     ribonucleoside-diphosphate reductase"
                     /protein_id="HDF2788651.1"
                     /translation="MIGWEMNEMQNAIWNNKYRNNNETFDEWLNRVSNGDKEVKRLIQ
                     EKKFLFGGRILANRGLQNDGRKITYSNCYVLATGDSIEDIYQTCSDTARTFSYGGGVG
                     IDISKLRPRGAKVNNSAKSTTGAVSFMDTYSLVAETIGQSGRRAALMISLDINHPDIE
                     EFIDIKTDLNKITKANISVRITDEFMQKATGIDSNPMYKCSFTREETGEIIVKEINAK
                     ELFNKLCENNWNYAEPGILFWDKINNYNLLSEDDEFEYAGVNPCAEEPLPAGGSCLLG
                     SFNLSEYVKEDKTFNYNDFRKDIKIVVKAMNDVLDEGLPLHPLKIQKDTVRNYRQIGI
                     GVMGIADMLIKMNVRYGSEMAIELCNVIGKCLADETLKQSALLSKKYGTYPKYKECIL
                     KSEFIQENASHETLELIKRYGLRNSQLLTIAPTGSISTMLGISGGIEPIFAFSYTRKT
                     ESLHDKEKYYKVYTPIVKKYMRENNIEDENTLPDYFVTATMLTPKERILMQSAFQKHI
                     DASISSTVNLPYEATIEQVEELYSLAWVNGLKGLTIYRAGCKREGILTTNTINNTQEL
                     KRGDWKPVSNDAVLYKKELYVGCGKLILFISYSETEEDIQELYVTKAGSGGCEKLLVT
                     ATIAMSGMLRLGGTLDNIEKALRGVNTCPSFATSRAKGNILDRGNHCGITILNAVKDF
                     LKEKQGEKIEESKEFNPKCPECGLEIQMMEGCMTCPNCGWSKCN"
     gene            15341..15703
                     /locus_tag="PC471_001517"
     CDS             15341..15703
                     /locus_tag="PC471_001517"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2788652.1"
                     /translation="MEFSEQIIKILDNLSDKVGMTIDWSNTNVIPYLQTLTTKYINYE
                     IATSVFWIIICVIGVILGILLIYMDWKYDNGFIVPGLCITILSFSCILIQSKDVITAK
                     IFPEKIVIDYLLEIKNNI"
     gene            15882..16289
                     /locus_tag="PC471_001518"
     CDS             15882..16289
                     /locus_tag="PC471_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2788653.1"
                     /translation="MYFETDIIKNKNTVNKIIKYCGGNTEKLKDWLKEIENKSISSLE
                     ELKEYPLKRNYKFVFSKEEAPNLDKLIKMKLRDNYLEKCEICSLENLLSMLNDEILDL
                     NKEDISYEDNIEWIENCKEELIKLKVGSICIDW"
     gene            16310..16621
                     /locus_tag="PC471_001519"
                     /pseudo
     CDS             16310..16621
                     /locus_tag="PC471_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009900673.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            16712..17062
                     /gene="tnpA"
                     /locus_tag="PC471_001520"
                     /pseudo
     CDS             16712..17062
                     /gene="tnpA"
                     /locus_tag="PC471_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003378761.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            17080..18519
                     /locus_tag="PC471_001521"
     CDS             17080..18519
                     /locus_tag="PC471_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015978935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDF2788654.1"
                     /translation="MSNYILTLQLKTEKFQEDIICNAFDKYRKVYNSCLSELFKRYNH
                     MIESKEHQANCKYKGKDRNKIFSEINKKYNLTEYSLHKFVKFIGKYYKLHSAITQKIA
                     TRCFNAFNKYIYHQAKRVNYIRYNELTSIEGKQNSTGISYKDGIIKFNKMTIPVIIKN
                     NDNYAQRAIQDKIKYCRILKKEIKGKIKWYVQLVLEGIPPKKTTNQGKIKGQIGLGNV
                     GIDIGTQTIAISSKCDVKLLELAPNVNNIDRQIKLIQRKMDRSKRATNLNKFNDNGTI
                     KQGNRDKWIFSNHYLKLKRLRKELYRKQAEIRKQDHYILINKLLNLGNKFYVETMSYK
                     RLQTRVKDTTINEKTGRINKKKRFGKSLANKAPSMFLTMLDNKLKYNNERLYKIDTAK
                     CRASQYNHFSDEYNKKELKDRWNNGMDIQRDCYSAFLIMNVNEDLKSINRELCVKTYD
                     NFKILHDKEINRLKKLKLNGYKLISSMGI"
     gene            18768..18944
                     /locus_tag="PC471_001522"
     CDS             18768..18944
                     /locus_tag="PC471_001522"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2788655.1"
                     /translation="MYDYGMSLSSIMKVILNHQEDIEKLKEEEVAEFIKNKLENEHDC
                     DELKIISEVFNEIY"
     gene            18931..19041
                     /locus_tag="PC471_001523"
     CDS             18931..19041
                     /locus_tag="PC471_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009900666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HDF2788656.1"
                     /translation="MKYIKHVDNGVMRAIGIDNYKPLLNIIKEKVNLESN"
     gene            19070..19537
                     /locus_tag="PC471_001524"
     CDS             19070..19537
                     /locus_tag="PC471_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009900665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2788657.1"
                     /translation="MGLDLGFYSVKTKVTDLDIYLNKTRGEKYKELNNDEEEFYKFEE
                     LLDIDISLEDWSVIRMIYKAKWEKGYESYEDEAILITKEDLEDYIIPFLKQKDINSKI
                     YNKDKEYYYKNDKKVTVDDRYTQEFWDLVLKEFQKMLNIIDFEKHSLIIMYWY"
     gene            19573..19953
                     /locus_tag="PC471_001525"
     CDS             19573..19953
                     /locus_tag="PC471_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009900664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-tRNA hydrolase"
                     /protein_id="HDF2788658.1"
                     /translation="MKKMYIAINSDLNMSPGKVGAQVAHAVYDYLYNKILDTVSCSYE
                     TEIIAHELIDLKCDLTSFKNNGDTICILKAKEAQLLKFKEKGYLTIVDRGLTEIPKNS
                     ITCVNLGIFNEDEIPSTIKRLRLY"
     gene            19969..20124
                     /locus_tag="PC471_001526"
     CDS             19969..20124
                     /locus_tag="PC471_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009900663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2788659.1"
                     /translation="MYSKGDVVKVWCYQCERIEFQEFVGYFNSYFDNYKLKKRWKCSK
                     CDVINNF"
     gene            20151..20597
                     /locus_tag="PC471_001527"
     CDS             20151..20597
                     /locus_tag="PC471_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2788660.1"
                     /translation="MKIKKIILGLDNLENVELGIGDVGGFYLDNIHEIIGGFTLSYIG
                     KCKQADYLHIQIHKRVDEIQRLHDYKDITNVKIEYEDGSVEDIYVPYKQEGDFLGLDN
                     LYQTSFISKQGYLHIVISKDKTVEEVFNEEFEDDEGVEFSFNMYGI"
     gene            20875..21039
                     /locus_tag="PC471_001528"
     CDS             20875..21039
                     /locus_tag="PC471_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2788661.1"
                     /translation="MTDKEMEEYIINLIKDKIKEEYGENILPEILMEKFIKLERKGSV
                     TVDVRDLIEG"
     gene            21170..21520
                     /locus_tag="PC471_001529"
     CDS             21170..21520
                     /locus_tag="PC471_001529"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2788662.1"
                     /translation="MCKFCDSIKDMKKHETRRVRISSVGIKGKDTTYESTELLDQEEY
                     FDISFYKFKEGNINLSIEHNLIVKDSNQNIKLNVDRFAGGIPFKYCFLCGEKLSSESI
                     DFENYIDNLMILEE"
     gene            21712..22179
                     /locus_tag="PC471_001530"
     CDS             21712..22179
                     /locus_tag="PC471_001530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2788663.1"
                     /translation="MFDMIINKEIFKYNNENIYDTLINSFSNETHEDDIKQGETIIIF
                     TTNKQLVYKELIRAAIKNKYTLIKHRNRIKHNIEIELKVLNTDRLIKFLIFDRDINYQ
                     ELRGYRGECVIVDVNTSNKTIQNFITEQIKPVLLFMNVYKKEYKPFHGVLLFK"
     gene            22199..22558
                     /locus_tag="PC471_001531"
     CDS             22199..22558
                     /locus_tag="PC471_001531"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2788664.1"
                     /translation="MNINTNYVSTNMIVMDISKGHNIEITKAMVEKSIHTFKNAPVIY
                     NPNQELSDYRDADRVNDFYKDEVVGIIMDVAIVDNQVIGKILWYDNCHIKEKYDNWYI
                     SLSDDKSEFILKEVEIS"
     gene            22610..23863
                     /locus_tag="PC471_001532"
     CDS             22610..23863
                     /locus_tag="PC471_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HDF2788665.1"
                     /translation="MEIKGKYNKAKIFTDNVDSTTISQIINLCNQKEFKESSIRIMPD
                     CHAGKGCTIGTTMTISDKVVPNLVGVDIGCGMATIPLEKYINNINYTKLDKIIRDYIP
                     HGFKIHNKSREKLLKNLFNIDINNLRCEVNKNRAYMSLGTLGGGNHFIEVDKSEKGQM
                     YLTVHTGSRNLGKQIADYYQDKAIKYCVEKYKKSYDDAKEFLISTLKKDNKEYLINKY
                     LKDLSDRKLNKPHDDLCYLENDLMEDYLHDMSIAQKYAAANRMVIIADILFKYNSLDV
                     NYDHFIRDIKCDVIECIHNYIDMGSKILRKGAISANKDETVIIPINMRDGIILGKGKG
                     NSEWNYSAPHGAGRILSRGKAKEKISLNEFEESMKEIFTTCVSQSTLDEAPQAYKPIE
                     DILNNIGDTIEIMEVLKPIYNFKSN"
     gene            24230..24409
                     /locus_tag="PC471_001533"
     CDS             24230..24409
                     /locus_tag="PC471_001533"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2788666.1"
                     /translation="MRLDDSQIGLIMYMIGFVHYDKEQFQFYMDAMYGKEYSEKEIVN
                     MLEHLRQDIKIQRFK"
     gene            24431..25750
                     /locus_tag="PC471_001534"
                     /pseudo
     CDS             24431..25750
                     /locus_tag="PC471_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897653.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
     gene            26096..26245
                     /locus_tag="PC471_001535"
     CDS             26096..26245
                     /locus_tag="PC471_001535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2788667.1"
                     /translation="MYIKINLLNDSIDKIQYDIAHGLLQSKEEKCIAKGKLSIINEIK
                     VQIYL"
     gene            26291..26641
                     /locus_tag="PC471_001536"
     CDS             26291..26641
                     /locus_tag="PC471_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2788668.1"
                     /translation="MKLINGNRSSGKTTALIKYAYENNALILCHSIANKNYIINLAKT
                     MNLDIINPRTFKEYMFNGSKIYDTKAINGISLKIDPHIKIVVDDIDCCLTSILGQHID
                     CATGTIQIENLNNI"
     gene            26653..27105
                     /locus_tag="PC471_001537"
     CDS             26653..27105
                     /locus_tag="PC471_001537"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2788669.1"
                     /translation="MIKYCKEKWIKNKTRLHEALLESDFINRDSYCSYEDLVKLSVKH
                     ILNDNINEDDEWDVDNISEIDNGHYQGTLLYLIPKKTYQPNEYEYLMTYVGYGSCSGC
                     DTLINIQDSDSEDSIEDYMCLCKDIICNIIKPYNFGWREDDKYNVVND"
     gene            27205..27489
                     /locus_tag="PC471_001538"
     CDS             27205..27489
                     /locus_tag="PC471_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5052 family protein"
                     /protein_id="HDF2788670.1"
                     /translation="MKRFLLIGVLGTMLIGMSGCTGIQSSMKDAESDVSGLNRTVNVY
                     SDNGEVLKTYTSKSMRVKDGDGGTITLDFDGKRVIICNAHVVIEENSSNK"
     gene            27501..27668
                     /locus_tag="PC471_001539"
     CDS             27501..27668
                     /locus_tag="PC471_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2788671.1"
                     /translation="MFKVLKTILIIIWVVDILNFPQFQFLDTTYPINTLAWLLIWILI
                     PSSSIYIDKKE"
     gene            27847..28173
                     /locus_tag="PC471_001540"
     CDS             27847..28173
                     /locus_tag="PC471_001540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2788672.1"
                     /translation="MRKVNVLENEKFEIKNYNIICAISMNHSKDVNQFDQYRLYDAQY
                     NLVEIDEKLKEYGYILIHTYQNGWDSWGDEYVCTVEDIKNIIKNAKNKEHLFIKALEE
                     YFGKQD"
     gene            28263..28865
                     /locus_tag="PC471_001541"
     CDS             28263..28865
                     /locus_tag="PC471_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009900658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2788673.1"
                     /translation="MIFISSTNRSNARDFHVSDYYRTPITKIKEFLTEFSRYENILNS
                     NIKILDPCAGGDNTNLMSYPTAIQEFSNQEISIDTVDIRDDSRANIKQDYLKFQPKEN
                     YDVIITNPPFNLSLDIINKALSDVREGGFVIMLLRLNYLGGKIRQELWRNNMPKYVFV
                     HSKRMSFTDDKKTDSIEYAHFVWQKGYNPKFSQLKVLISQ"
     gene            28880..29089
                     /locus_tag="PC471_001542"
     CDS             28880..29089
                     /locus_tag="PC471_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2788674.1"
                     /translation="MKVKYVVFEWEITSKNDGQKHFINFRDLIKLYGVSPRECIRAKD
                     YYERDGLDLKDIKFLYPRDDGKYKL"
     gene            29144..29389
                     /locus_tag="PC471_001543"
     CDS             29144..29389
                     /locus_tag="PC471_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2788675.1"
                     /translation="MDIKIDMCLLEMEINRRRCEYAARTGRYPNSLILWEGYYSLLNI
                     QYIEDKTRGYITKFNGMDVIKCNDFLKIELFEKNINN"
     gene            29418..32489
                     /locus_tag="PC471_001544"
     CDS             29418..32489
                     /locus_tag="PC471_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019595671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HDF2788676.1"
                     /translation="MRYNNYHSHKIYSNIRSLDVITKPQQYIDRAIKLGQNTYFTTEH
                     GYQGNVYEAKTLCDEYKLKMIIGAEFYYVNDINEKDRGNYHLIVIAKNNDGYKQINKA
                     LSLANKNGYYYKPRIDEKILFEIFKPGDVVITTACVAGILKLENREELILKLKNYFKN
                     NFFLEVQSHPHKTQALHNKDVLELSNKYNIDIIHANDSHYIYPEESKYRTKFLKAKGI
                     NYPEEDGFILDYPNSEDIFKRYEKQNVLTKQEVERALKNTLIFDECEEITLINDDIKL
                     PSISKNPNKELKEILNKEWLEKRKNIPKNRWNEYLDAIRYEFDIIEKTHMEDYFIIDY
                     KIVQRAKQEYNGLLTKTGRGSAPSFIITNLLGLTEIDRLNAPVPLFPTRFMSVERILS
                     AKSLPDIDLNAEDAEPFIQATRDLLGEENCAWMISYKPLQDASAFRLWCKANDMKLSE
                     YDEVAKNLDKYTNDVFWKDVIKESKVFVGVVESVSFSPCSMLIYDRPIDEEVGLLKTK
                     DGVCCNIDGYYCDKYKYLKNDYLTVKVWSLIRKICELANISIPTIEELNILLDAKTYD
                     IYKDKLTCTINQVDSDYATNLASKYKISSVAETSAFVASIRPGFASLLDNFINRESYT
                     TNVKELDILLEDSYHYLMYQESIMKYLIWLGIEESESYDIINKIKKKRFKEKELKELH
                     IKLKNNWIKKVGTENNFEDTWQVVNDAASYSFNASHSLSYAYDSLYCAYLKSHYPLEY
                     YTVAFNLYNEDTERTRKLTDEIEYFSIKLENPKFRFSKSEYFFNRDTNSIYKGIESIK
                     FLNSDIGEYLYSLKDNKYDSFLELLIDLQGHINSKQLSILIKLDFFEEFGKSNKLLET
                     YDIYNSIYSKKQFKRDNLPCNINTMRKYSNKETEKIFKEVDTKELCSYLESQIENTDI
                     PTNEKIQAHFEFVGSCNIKDSNSNPRYCLVIDIDTKYSPKVTLYNISSGNIKIFKVSK
                     PIFEDNKIDVYDLVYLKSIKEKAKSKKVDGKWIRSNTETEWWIQEYWKIEN"
     gene            32592..32843
                     /locus_tag="PC471_001545"
     CDS             32592..32843
                     /locus_tag="PC471_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2788677.1"
                     /translation="MISILIDEQRYYSLLEEKKDLSMRIDELKEENAFLKQENEEYEE
                     ILNEWECVRKEIVATCKKKEILGEKPSELEMVLQKIEDI"
     gene            32843..>33069
                     /locus_tag="PC471_001546"
     CDS             32843..>33069
                     /locus_tag="PC471_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2788678.1"
                     /translation="MSVNKIYEKKEYIIIKSKSGYIVYNTNKEFKEGHTHLKSYNAAK
                     TAIDLVIKRRLPKSYSIYFLTSLMRISNDVQ"
BASE COUNT        14269 a         2834 c         5668 g        10298 t
ORIGIN      
        1 agtgatttaa tgattataac tatttgcgaa aatgatgata tgcaaagaga tatcatctat
       61 gattttgtta agatattttt aactaaaatc aaggtaaaac attatgacat tttaatgttt
      121 aagaatgggg aagaactgtt agaagaatat ccaaacaaag tggatttaat tttaatggat
      181 ataaatttag gaggtagaaa tggatttgat atttcaaaag atattagaaa atttgataaa
      241 gatgcaagaa taatttttgt aactgtatca gagaaatatg ctatcaaatg ttatgagata
      301 aatgcattta gatatatgat aaaaccgata tattataaag aatttgaaaa atatatgctt
      361 gactttgcaa ttgattggag aaaaagagaa gctattatta ctataaaaag aaaaaaacaa
      421 ataataaaaa tagaggaaca taatatttta tttgttgaaa tagtgaatag aaaattaaga
      481 gttgttacta aaaatgaaga acatataata aatgataaaa tggatatgtt tgaagaaaag
      541 ttaaaagagg atatattttt tagatgccat aagagctatc ttataaattt acttgaggta
      601 actaatttca attataattt cgtttattta acaagtgaga aaataaaaat taaagtagct
      661 cctaaaaaga ccaaaaaact taaagagaaa ttagcaaatt tttttaggga aataatatgg
      721 taaattaaag aaaaatttct ataatcgaat atatgttctt gataaaagta tgtgtacaag
      781 atataatatt attagtagga aaataggaaa aatattaata tttttgaaca ttttgacatt
      841 aattgtgatt ttagtgttga atttctgtta aaaaaatctt attatttaat catagaaatc
      901 tactgttata gagtagaaga ttgttaatta taaatgattt taaattatct gagataaaat
      961 tttttgtttc tgaaaagaac gaatgttcga atgaggtgta gagtgagagg atgtttatta
     1021 aaagatatta gattatctta cttaattcat aatgcatata tgggggttct ataataatga
     1081 aaaaacaagt cacaaaagaa atacttaaaa tgctagaaaa aatggattta tcttatttac
     1141 tcataattaa aagttttgta tctaaattaa aatagccctt attgggctat ttactaagtt
     1201 tatcaattac tttctgtatg acttctcttt catcatcagt aagttcagca aatgttaaga
     1261 atactttttt taaaaattca tcattacttg agaatgtttt acccatcatt gctgctaatt
     1321 caatatcatc tatattttta aacatttctc catttccatt tcgtaaccag tcctcattta
     1381 cattaaaaac ttcacatatc tgaaatataa atctatctgt tagctttctt tctccttttt
     1441 caagtctaga aatagttgca tcacttacta ataatttttc tgcaaattct ttttgtgaga
     1501 gacctaattt aattcttaat tcctttaaat tctcattcat atttacacct cctattatat
     1561 agtatattaa taaaaattga caatgtcaat aaaataggaa tgataaattg caaaaagcaa
     1621 aaaaaataat tgacaaatac agaatgtggt actacaatgt aaatatacaa aaagcaatta
     1681 ataatttgac aaacgcaaat aaataaggag gtagattatt tgtataataa taatgataaa
     1741 aaattaataa atttatttga atcatttaaa aaaatagact atacagaaca aagtttagtg
     1801 tattttttat ctttaagctt tatagtaaac agaaaatgtc ataaagatgc aaatttgtat
     1861 agtaggagag gtgaatgtaa ttatgaataa tttaaatatc aaaggaaaac aaaaatttat
     1921 ggatttagaa atacctatta tagaaggagg ttttggagag ggtaaaaaat gtataacaga
     1981 taaaactata tcagaaatac acaatatgga gataaaacat gttagagaat taataaatag
     2041 gaacataaga agatttataa atgctattga ttttatagat ttaaaagtgg tcgttgataa
     2101 cgactacaat ttagaagtat tggagttgct tagttataga aaaatgcaaa tatcaaaagc
     2161 aaataatatt tatcttttat ctgaaagagg atattcaaaa ttaattaaga taatggatac
     2221 tgatttagct tgggagatac acgataaaat aatggatgaa tacttcaata tgagagaaca
     2281 actcatagat agttatatga tacaagattc tattgaaaga gctaaaagat ggatagaaga
     2341 agaaaaagaa agattattac ttaaagaaac aattcaagtt caacaaccta aagtagaata
     2401 tcatgataaa gtattaaatc ctgacaaatt agttacgaca acagatattg cgaaagattt
     2461 aggaatgaca gcacaagcat taaataaatt actacattta aacagaatta tatatccaga
     2521 aagaataaat ggtcgaataa aatgttggaa accatataaa aattatttat ggttgatacc
     2581 tgaatatgca gattttaaaa taacagaaca tgatacgcag cttaaatgga ctgagatagg
     2641 tagaaaatgg ttaatagaat ttgtagagaa cattaaaaaa ataagttaaa tcaagaagtt
     2701 aaaatgtacc ttgaaaagta gatatttaaa aatataaaag ataggtgatt aaatgataaa
     2761 acaaatacta aataaaagag gtagtctatt aaatctagca agtgagtatt tgacagaaca
     2821 agactacctc aagtttcaag gatggtatac acatagatat agtaagctgg atgcttatga
     2881 tgcttctata aatgaatatt taaattataa aaacgacaaa aaattaataa cttatgtatt
     2941 taatttaaaa tttcaagaag ctctcataga aatataatat tactaaacat ggtgattgaa
     3001 atgaattata attatgtaac taaaattgac ttgaaatatg gaaatacaca atttgggttt
     3061 gagaaatata gcgatgcttt agataagtac agagaactta aagaagaatt taagtatgtt
     3121 ccatgtatta taaaacttca ttcatatgta gcgcaccaag atttatatgt gaaagaaatt
     3181 aaagctgatg gttttgaaaa gaaatattta gatattaaaa atttaataaa attgtttaaa
     3241 gaacataaac aaaatatatc agatgaacta agtctttgtg ataagaaaaa ggaagaagta
     3301 ttacatgtaa tagaagaaat gaatttagac acagcttctt taaatgataa gattgatacc
     3361 attaaagaat taaaaaacac tttagttaag agaaggttgg ttaaatatga actaaataaa
     3421 tactttgcct tttgtaatac attatatgaa attgaagaag agttaaaaaa atatgaatct
     3481 aaatataaac aaaaagaaaa tcaaggaaaa agcagattca aaaaaataaa aaataggtga
     3541 ttagatggaa gaatatcatt ttaacttaat aaaaaagctt tgtgctgaaa gaaaaataca
     3601 caaaggttgg tttagaaata ttgaaaaatg tggttgtgga ggtaaatgta ataagaaaaa
     3661 ttgtaagatg ttagaaaaat tataaggaga tacatatgga aaatcaaatt gaattaacag
     3721 aaaaagatat agaaaacact ttaagtgctt taaatattat taaatatatt tgtataaaat
     3781 ctaaacattg caaaaattgt cctcttggat atatgaaaaa tcttgatgaa cataattgta
     3841 tgctatatga attaatagac aagggtttac ttccatgtga ttggtctgta aaaaaagtta
     3901 caagattgtt agatacgaca gaagaataaa acaaaggagc tggttaagtg gagttagatt
     3961 tcagaatttg gaatggattt agaatgatac atggagaaga attaagatta ctaacagaag
     4021 agatgtttat aaaaagtgga tattctgaac aatactataa cgatatttgt ataccaagag
     4081 tagaagattt gaaaataatg caatacatag ggctaaaaga ttgttttgaa aaaaagatat
     4141 ttcaatatga tattgttaaa tttatctttg atggtgtttg gaaagtatgt ttagtaaatt
     4201 tatctttgcc aaaaggagca acagttacat ttgaaagcat aggtggaaaa tatactttta
     4261 atttaagtga aacattttca aaagatagga aattagaaat cataggtaat ttttatgaaa
     4321 ataaagatgt aatcgaaaaa tataatctta gtattgaata ggagaagaaa atggatgaaa
     4381 tgataattag ggtgaaaatg ggagaaccat taccaacact aggaaagata atcaaatgta
     4441 ctacatgttt aactaatgaa gagaaatgtt ttaaggttaa taagatatgt aaattaagtt
     4501 ggattaacga taggaatagt gaagggttaa ttgtagaatg caaaggtaaa tatattaaat
     4561 attaataaga ataatgaagt taaaatcaag attttaatag aaaaggagtg attaaaaatg
     4621 aagaaaaatc caataaaaat taaaaaatgt gactgtggtg gagatatata tgaacttaat
     4681 acacaattag gcactatgta tcaatgtaaa agttgtggcg agatgtatag atagtgctga
     4741 aatattagct aaagaagatg aggtgaagtc aatgagtaaa aaagataagt tatctatttt
     4801 tataagttta atattaagac ataaaccaga aactattgga attaaattag atgactatgg
     4861 atatgcagat gttaatgaat tgattgaaaa aataaataat actggtagaa atattaatat
     4921 agaaatcctt gagcaaatag ttaaagaaga taataagcag aggtacagtt ttaatgatga
     4981 tagaagcaag attagagcaa atcaaggaca ttcaattaat gttgaggttg aattgaaaga
     5041 attagaaccg ccaaaatgtc tttatcatgg aacggctact agatttttag agaatattaa
     5101 aaaggaaggt attattagac aaagtagact gtatgtacac ttatcaaatg atatagatac
     5161 ggctgttaag gttgggaaaa ggcatggtgt acctgtagtt ttaaaaatta atacaggaaa
     5221 aatgtatgaa aatggttata aattttattt atctgaaaat aatatttggt tatgtaagta
     5281 tataccattt gaatatgtag agaaaataag tagagtagtt aaataaaatt atagaaaata
     5341 ggaggttatt gaatggattt caattttaga aagatttgtg aaaaagatga acttactgat
     5401 gattttatta taaaaatttg ccagttgtca catgaggagt ttggaggcac tatttatgta
     5461 ccatgtaata ttcatccatg tgggtgggat gagttggaag ttccgaatcc aaaggattta
     5521 gattcagata aattaatgga agaattttta ttagaatata aatttgaatg tgaaaatatt
     5581 tcgagcaata tttatgatag tgaccatcca tttataggca aaacattgag agagattctt
     5641 atgatagtgg attgtagatt cataattggt gttatataag gagggtatta aatatggaaa
     5701 atatagaatt aacacctaga gattattttg aggatataaa aaataaaaag caaaaaaata
     5761 caaatgaaaa tcttgatata gtatatgaaa actctttgat tcttttaaac aaatatattg
     5821 taactggtca aaaaaagggt gctaagaaat taatgttttt acttgaatgt attgaaaaag
     5881 aaagaaatgt aatagagctg ggaatagata catttgtgta taaagaagat atagaaaaat
     5941 atattgatga tatatctaaa aatacagtaa aaataataga acttgaaaat tatgaaagag
     6001 aaataccaga tgaaatagta gaaataatag aaaaaacaaa agatatattt gataaaatgt
     6061 atgttttgtt tactgattat acaggtaaaa ttacaaagga gataagcaaa gagagaagag
     6121 ataaagaccc aatattattt ggtgtttttt cagatgagaa aagcagagct attgtagata
     6181 gattttatta tcttggagat tgggaggatg agtattgtga tttaacatta gagaaaatga
     6241 taaatgatta taagaaaaaa ggaagacaag acatattaaa acatataatt acacctaaat
     6301 cattagatga gttaaaaaaa gaattagatt ctttagaaaa aaataatgat acatataagc
     6361 aagttgccaa tataaagcca ttacctacta caaaaaactc tagtgtagaa ccacctaaat
     6421 taaatatatt taaaaaaata aaaaatagag tgtttaaaaa tgaaaagtaa tgttgattta
     6481 acagagaata gggattttag agatgtaaga ggaagaggca tcaatatttt aggtatcgaa
     6541 gatgcgccaa atagaatgtt gagttttagt gattttggaa ttggacgtct ttatggtaaa
     6601 aatttagaat ttgaatttaa taaaaatgaa gtatttttaa ctggaaatgc taatgacaga
     6661 gaagaaaaaa agtttcttat cctttgtgaa gaaaataagg tttgtgttag atgtggtgat
     6721 gaattaagag aggggtggga taaagtaagc atagaactat gtaatgaatg ttatgaaatg
     6781 ttagatgttg agtataaaaa taaaaagata attagagaat tattttttag agatagagat
     6841 gtaaatatag agttacttta aaaaatatat aagaaaaata agtagaggag gaatttatat
     6901 gaaagatgtt tgattatata gctgggatga tgagtgcttt ggaagtgatg aatacgaaag
     6961 tagagaggaa gctattcaag gagctaataa aactagtatt tttagtgatg acaacagtat
     7021 gacaagaata atgagaatat aaaggagtga tgtttattaa aagttttaga tgtatattca
     7081 gacataagtt ttcaaggata aaagattgtc aattaattac aacaaggagt cagcaatata
     7141 cctgtaaaat ttggatgtgt aaatattgtg gacaaattgt agctggttca actcctgttt
     7201 atcatcctaa tatgaaagtc aatgagtatt gtgtaaagtg tgagttttcg gcatgtgcaa
     7261 ataattatga gggatattgt aactctaaag ataaagaaat tgaatttaag tctatttgct
     7321 taaaaggtaa taaagagatt ttgcagtgta aaaattttga tttggaataa aatttagctt
     7381 aagtaggaga attttagaat atgaaaataa atgtacatgt taaaactaat aaaattggtt
     7441 cagactgtga gctggagttt gaaatagatg atgatgattt aaaagatatg tctgatgaag
     7501 agagagagga ttatatagat aaaatcgcat gggattatgt ttttgaaaat tttatagatt
     7561 ggaattggta tgaggtcgaa gattaagtta aaacaatagt tttaattatt tataaaggag
     7621 gatttaatgt gaataaagat aatttagaaa caattataga aggttataag gtatttaatc
     7681 cagattggac ttgcagaaat tttaaatatg aagtaggtaa gacttatgaa catgatggag
     7741 atatagaagt atgtgaagct ggatttcatt tctgtcaaaa agcaattgac tgttttaact
     7801 attattcttt tgataataaa aataaagttg ctaaaattga agcaataggg ttaattaaga
     7861 ctgatggtga taaatctgtc acaaataaaa ttacaatagt gaaagaaatt gagtgggaag
     7921 aattattaaa tattgtaaat atagggaata acaatacagg atatggtaat acaggaaatc
     7981 ataatacggg aaatcataat acaggaaatc ataatacagg atatcgtaat acaggagatt
     8041 ataatacagg aaatggtaat acaggatatc gtaatacagg agattgtaat acaggatatc
     8101 gtaatgaagg aaattataac gcaggaaatt ataacgcagg atattgtaat acaggatatt
     8161 gtaatgaagg aaattacaat acaggaaatt ataactcagg gaattgtaac tcaggagatt
     8221 ggaacatagg agattataat tcaggggtat tttgtacaga tgttccaaaa atcaggatgt
     8281 ttaataaaga aactgattta acatatgagg attggatgaa tagtgatgca cgaaatattt
     8341 taaaaagaaa tagttattta acaaaatgga ttcacattga tgacatgaca gaagaagaaa
     8401 aagaaaataa tccaggatat gaaattaata atggttattt aaaggtgttt atatttgagg
     8461 agatgtgcaa gaacatgtgg gatagcttaa ctgaggaaga aaagaaaatt attatagaaa
     8521 taccaaattt tgatgctgat atttttaaag agataaccgg aatagaagta atttaaatta
     8581 aaaataaagg aatgcaacat agtggataaa caagaatata caaattgttg tggtaattgt
     8641 aaagaatgtg atgaatatca taagatgtta tgtaattcat tatatgagga ggaataagaa
     8701 tggctaaata tgatttattg ctaaaattaa ataatatttt attcaagatg gaaaaacatt
     8761 gcgatgaaat gattaaaaat aataacgata aggaattgag aacctgtact aattgcgaat
     8821 ttgcttatta tggtagttat gttgaaagtg gatgtgtttg tacagatatt gaaggatttg
     8881 gcatacatga tatggtaaaa agaattattg aaaaaaataa agaaaagatt gatttagaaa
     8941 gaaaaataaa gtatccagct atatataaac attttaaagg cggattttat acagttatgg
     9001 gaattagtaa ggctgttact ggtgatgaac ttgataatat atttaaaaat atgggatgca
     9061 taaacagact ggatgtactt gaatatagat ttggttctag gcatacagaa actaggcata
     9121 cagaaactag ggaaaatgta attatataca gagacaataa aggcaattta tatcattata
     9181 aagatgttga caataataca aatttagtaa tatataaaac tttatatgat ggaactggag
     9241 cttatgcaag acctattgaa atgttttttt ctaaaattga taaagaaaaa tatcctaatt
     9301 atactcaaaa atacagattt gaagaggtgt aaatgtgcca acatttaata gatatagaat
     9361 agaagattta agtggattgc aaaatgaaag gtggagaagt gaagactcca gaaatatatg
     9421 cttagctgat tggattcctg gaaacatctc acacaattcc aatcctacaa ttgttgacca
     9481 atttaatgat gaacttggag gtctagcaaa tgaatttaca caatggaata tgggagattt
     9541 ttttgctcaa tttactccaa taaaaaaagt taaaatagga gatgtggcaa gaaaatattt
     9601 gaatccgcat tattatattc ctgatgaatt taattttaat aatattacaa atgatgagag
     9661 tctatatttg ggaatagaat tagaagtaga taatggtggt aaaagtgaag aaaatgcaaa
     9721 gtttattcag gaatttttag gagaaaagaa ttgctatatt gttggtgatg gaagtttact
     9781 tgatggctta gaaatagtaa ctcatccttg taccctacaa tatcataaaa aattagaata
     9841 taaagattta tttgaagaat taaaagaaaa gggatataaa tctcatgata catcaacttg
     9901 tggttatcat atacacatta atagagattt ttttagcagt gatttaacta tacaagactt
     9961 atgtattaca aagattttat atttaataga aaaatattgg aaatatgtaa cagtaatagc
    10021 tagaagaagg tcaaaccaat attctaaaag atttggaatt aaagataatg aaagtatgtt
    10081 tgaactttta attaaagcaa aggggagtta cgtatctaaa tacaatgtgg ttaatttaca
    10141 acatagtaaa acaatagagt ttaggatgtt tagaggaaca ttaaaatatg aaacttttat
    10201 agcaactatg gaatttgtac ataatttagc ttatatatgt aaagatacat ctttagaaga
    10261 tatacaaaat atagaatttc aagatatatt aaatgtatat caaacagaat atctattgtt
    10321 atattttaat aatagaattg aaaatatgaa taatactgtt gatttagaag atgaatataa
    10381 agaaaatctt agaaggagaa tttttattag agatgaatgg tcactttttc atgagagctt
    10441 aatgtaataa atataaatta taagatacta aatagctcat aatacgtgac ttactatata
    10501 aggaggtata attttggaca ttaaatcaag gttagaatca aaagaatata attttcttag
    10561 agaagaagaa agattaaaaa atagaatgat acttcctcca attgtaagaa gttttgcata
    10621 tggaactaat atagatactc ctgagcatat atctgattta gacataagag ggatatattt
    10681 aaattcagta caagaaatat taactatgaa ttgtataaat aagccatatg aaaataaaca
    10741 aacagataca gttatatatc cattaaagca aataataaca ttattattaa atgtgaatcc
    10801 aaatgtaata gaaatattag gtacgagaaa agaagactta tttgtttgca ctgaagaagg
    10861 gaagctttta aaagataatg tagatttatt tttaactaaa agagctgcta atagttttgg
    10921 tggatatgca atatctcaac taagaagatt acaaaatgct ttagcaagag ataattatga
    10981 acaaaaagaa aaagaagaac acataatgaa cagtgtaaaa aggcaaatga aaacatttga
    11041 agatagatat aaagtcgtat ctgaggaaga aataaaattg tatattgaag aatctaaaag
    11101 aacagattta gaagaagaaa tttttataga tgttaatctg agaaagtatc cacttagaga
    11161 tttaaaaggt atgtggagtg aaatgaataa tatactaaaa gattattcta aattaaatca
    11221 tagaaataat aaaaaagatg aattacacct taataaacat gctatgcatt taattagatt
    11281 atttttaatg ggtatagaaa tactagaagg aaaagggatt aatacatata gagagaatga
    11341 taaggagttt ttattagata taagaaaggg aaaatatacc tataatgaaa tatttgagat
    11401 ggtaaatgaa ttagataaaa actttaagta tgcagaaaaa aattcttgtc tacctaattc
    11461 tccagatatg aaaaaagtaa atgaactggt catgtatatg actaatagag tattaaacag
    11521 atagataatc aaagaggtat aaacatgagc gtaatcaata aaaaaaataa agacattcat
    11581 ttttatgaag agaaaatatc tattcctaat gagctataga tttattttaa taagtggttg
    11641 gaaactagca aaatcaaata aggaggtatt agtttgaata taaataaatt aaaaaaaata
    11701 aaagaaagga aaacaaaaag atggaatata gaaaaaatta ctaataggat aaatgaccat
    11761 atgaatattc aggaggatat agaaaaatgg gataaatttg aaacaccaat tattagtaag
    11821 gaggtagatt tttatttatt gaaaatagga tttcaatatg gaaggaataa tgggaggaat
    11881 gtcttggtag gaattaagga tatgcgtcat atatcaataa gtaaagttaa ttctaactta
    11941 ttatcaatag atatatataa atttattaaa caagaagatg gcaaatttag tgaatttaaa
    12001 gcacatgaat atatagaaaa gtaattaaaa catttgtttt aatgtgattt tacctagtag
    12061 gtacaaggag gtttttaatg aaagagcttt tagaagtaaa aaaaatattt gatagtttag
    12121 catctacatc tagtagaaaa gaaaaagaga gaatattaga aaaatataaa aataatagaa
    12181 tgtttgttga atgtttacaa tttctgctag attcaaatat tctcactgga atatctaaga
    12241 ataaaatatg taagaattta aataatacta gtcataatga attagagaac atatatgata
    12301 tgcttgacta tttaattaag aataatactg gcagaaatat agatgtaaaa acaatacaag
    12361 tatttgccag taaagatgaa aaattaaaag attttatttt taatttagct acaaaatcaa
    12421 ttagattagg aataacatat aagactgtag acaaaataat gccaggttta attatctaat
    12481 aaattttgta ttttttacaa aaaactattg agaacataga ggttatatga tataataaaa
    12541 atatattgag aacagagaag ttaaaaaagg gggcaatgag atgactgaca aaataataat
    12601 agcaagtgta gatggagagg aaatgatatt tagaactgaa atgaaaaagt tagaaaaagg
    12661 agatagtctt atagtttctg taaatggtgt aaaaagatta gctctattca ttaggtatat
    12721 aagtacttgt gaagaatata tagaagatat agcatttcaa aaagtagatg aaatattata
    12781 tgaaaacaca attgccaaag aaaaagaaag agttaaaaag gtatttaaag ataagtgtat
    12841 gaaaaattat aaacctattg ttaatacaga aatagactct tatttgaggg aatgtggttt
    12901 caattgtgat ggtagacaat ttttcaaaag tatagacaat acacaaattt taattagcag
    12961 ggattgtgat aaagatgttt tgactgtatc gaaaggtttg acaataacta aaacagatat
    13021 aacaaataaa aatttaatta cagttattaa aataataaat aatagtctat taatagttag
    13081 gtaatagaaa taaatatttt tataataaaa gggagtgatt taatgatagg ttgggaaatg
    13141 aatgaaatgc aaaatgcaat atggaataat aaatatagaa ataataatga aacttttgat
    13201 gaatggctca atagagtgtc taatggagat aaagaagtaa aacggttgat acaagaaaaa
    13261 aaattcttat ttggaggaag gatattagct aatagaggat tacagaatga tggaagaaaa
    13321 ataacctatt caaactgtta tgttttagca actggtgatt ctattgaaga tatataccaa
    13381 acatgtagtg atactgcaag aacatttagt tatggaggag gggtaggaat tgatatatct
    13441 aagttaagac ctagaggggc aaaagtcaat aattcagcta aatctacaac aggtgcagtt
    13501 tcatttatgg atacatattc cttagttgct gaaacaatag gtcaaagtgg tcgtagagct
    13561 gctttaatga tatctctcga tattaatcac ccagacattg aagaatttat agatattaaa
    13621 acagatttaa acaaaattac taaagctaat atatctgtaa gaataacaga cgaatttatg
    13681 caaaaggcta caggaataga ttcaaaccca atgtataaat gttcttttac gagagaagaa
    13741 actggtgaaa ttatagttaa agaaataaac gctaaagaac tatttaataa attatgtgaa
    13801 aataattgga attatgcaga accaggaatt ttattttggg acaaaataaa taattataat
    13861 ttattgagtg aggatgatga atttgaatat gcaggcgtaa atccctgcgc cgaagaaccg
    13921 cttccagcag gtggtagttg tcttttaggc tcatttaact taagcgaata tgttaaagaa
    13981 gataaaactt ttaattataa tgattttaga aaagatataa aaatagtagt aaaagcaatg
    14041 aatgatgttt tagatgaagg tctacctttg catccattga aaatccaaaa agatacagtt
    14101 agaaattaca gacaaatagg tattggagtt atgggaatag ctgatatgct cataaaaatg
    14161 aatgtaagat atggttcaga aatggccatt gaattatgta acgtgatagg taaatgttta
    14221 gcagatgaaa cactaaaaca atcagcctta ttatcaaaaa aatatggtac atatccaaaa
    14281 tataaagaat gtattttaaa atcagaattt attcaagaaa atgcatctca tgaaacacta
    14341 gaattaataa aaagatatgg tcttagaaat tcacaacttt taacaatagc tccaactgga
    14401 agtatatcaa ctatgctagg aatatctgga ggaatagaac caatcttcgc cttttcttac
    14461 actagaaaaa ctgaaagttt acatgataaa gagaaatatt ataaagttta cacacctata
    14521 gttaaaaaat atatgagaga aaacaacatt gaagatgaaa atacacttcc tgattacttt
    14581 gtaacagcaa caatgctaac tccaaaagaa cgtattttaa tgcagtcagc atttcagaaa
    14641 catatagatg caagcatctc ctccactgta aatttaccat atgaagcaac aattgaacag
    14701 gttgaagaat tgtattcatt agcatgggtt aatggattaa aaggattgac aatatataga
    14761 gctggttgta aacgtgaagg gattcttaca actaatacta taaataacac acaagagctt
    14821 aagagaggag actggaaacc tgtatcaaat gatgctgttt tgtataagaa agagctatat
    14881 gttggatgtg gcaaattaat tttatttata tcttattccg agacagaaga agatatacaa
    14941 gagttatatg tgacaaaggc tggttcaggt gggtgtgaaa aacttcttgt gactgcaaca
    15001 atcgcaatgt ctggaatgct tagacttgga ggaactttag ataatataga aaaagcttta
    15061 aggggtgtta atacttgtcc tagttttgct acaagtagag caaaaggaaa tatattagac
    15121 agaggaaatc attgtggtat tactatttta aatgctgtaa aagattttct taaagaaaaa
    15181 caaggagaaa aaatagagga atctaaagaa tttaatccaa aatgtcctga gtgtgggcta
    15241 gagattcaaa tgatggaagg atgtatgact tgtcctaatt gtggatggag taaatgtaat
    15301 taaattaaga gggattactc cctctttgga ggtgaattaa atggagttta gtgaacaaat
    15361 tataaaaatt ttagataatt taagtgataa agttggaatg actatagact ggtctaatac
    15421 taatgtaatt ccatatttac aaacattaac tactaaatat attaattatg agatagctac
    15481 aagtgtgttt tggataatta tatgtgtaat tggagtaatt cttggtattt tattgattta
    15541 tatggattgg aaatatgata acgggtttat agttcctggt ttgtgtatta caatactttc
    15601 tttctcttgt atattaattc aatcaaaaga tgtaataact gcaaagatat ttccagaaaa
    15661 aattgtaatt gattacttac ttgaaataaa aaataatatt tagaggatga ttgttatgac
    15721 caaaaaagaa tttactacta aatttcttaa agagcagcag agaagaataa aggttttata
    15781 ttatgaagtt gaaaatttca agatggatag aaggtttaat gagaggaaaa agtctaggag
    15841 acattaaaat gtttctttta atataaggag gaagtagcta tatgtatttt gaaacggaca
    15901 ttataaaaaa taagaatact gtaaataaaa taataaaata ttgtggtgga aatactgaaa
    15961 aattgaaaga ttggctaaaa gaaatagaaa ataaaagtat cagtagttta gaagaattaa
    16021 aagaatatcc attaaaaaga aattataaat ttgtattttc caaagaagaa gctcctaatt
    16081 tagataagct tataaaaatg aaactaagag ataattattt agaaaaatgt gagatttgta
    16141 gtttagaaaa tctcttaagt atgctaaatg atgagatttt agatttaaat aaagaagata
    16201 tctcttatga agataatatt gaatggattg aaaattgtaa agaagaacta attaaattaa
    16261 aggtaggaag tatttgcata gattggtaga aaaataaagg agtgatactg tgagattttg
    16321 gtgtgagaat tgtaataaat acttcaatgc ggaagaaaca ttacaggaat ataattattt
    16381 tttaaatgaa gagatactta tatgccctac ttgtaaaaaa gatttaatac ctatagctag
    16441 taaaacggaa ttatcattag gctttgatag tgatacaaac caattagctt atgttgaata
    16501 tgattctagt gattatagtt tacttagaaa ggtaaatgca gatgttgaag atgtagtaaa
    16561 acctataata cattacataa gaaatttaaa taaaaatagt ttagatttaa atggaattac
    16621 agaatagtgc ggaaagccca ctcctttagg tgtgggatgg atagcacaaa cagtgtataa
    16681 aaagtattga cacatacacg tataatgtta atactttgta ttctgcccta gatataaaag
    16741 aaaaatattt aatatagaag gtttagaaga tagatttaaa acattagtta aaaataagtg
    16801 taaggaaatg gatatcgaaa ttgttgcaat agaatgcgat aaagaccata cacatatgtt
    16861 tttaaatagt cttccgactc taaatccttc tgatataatg caaaatataa aaggctatac
    16921 aagtaaaata ttaagagaag aatttattga attatcaaaa atgcctagtt tatggactag
    16981 aagctatttt gtatctacag caggaaacgt atgtagtgaa actattaaaa aatatgtgaa
    17041 aaaccagaaa acaagatact agaaagtgag gtgaacacta tgtcaaatta tatattaaca
    17101 ttgcaattaa aaacagaaaa gtttcaagaa gacattatat gtaatgcttt tgataagtat
    17161 agaaaagtat ataattcatg tttatcagag ctatttaaaa gatataacca catgatagaa
    17221 tctaaagaac accaagcaaa ttgtaaatat aagggtaaag atagaaataa aatatttagt
    17281 gaaattaata agaagtataa tttaacagag tattcacttc ataagtttgt taaatttatt
    17341 ggtaaatatt ataaactaca ttctgctata actcagaaga tagctacaag atgttttaat
    17401 gcttttaata agtatatcta tcatcaagct aaacgagtta attatattag atataatgaa
    17461 ttaacatcaa tagaagggaa acaaaattca actggtatta gttataaaga tggtattatt
    17521 aaatttaata agatgactat tccagtaata ataaagaata atgacaatta tgctcaaaga
    17581 gcgatacaag ataaaataaa atattgtaga atacttaaaa aagaaattaa gggtaaaatt
    17641 aaatggtatg tacaattagt cttagaagga attccaccta aaaaaacaac caatcaagga
    17701 aaaataaaag gacaaattgg tttaggtaat gtaggtatag atattggaac tcaaactata
    17761 gctatatcta gtaaatgtga tgttaagtta ttggagttag ctcctaatgt taataatata
    17821 gataggcaaa taaagttaat tcaacgtaaa atggatagaa gtaaacgtgc aactaatctt
    17881 aataaattca atgataatgg tactataaaa caaggaaata gagataagtg gatatttagt
    17941 aatcactatc ttaaattaaa aagactaaga aaagagttat atagaaaaca agctgaaata
    18001 agaaaacaag accactatat attaattaac aaattactaa atttaggcaa taagttttat
    18061 gttgaaacga tgagttataa aagacttcaa actagagtca aggatactac tattaatgaa
    18121 aaaactggta gaattaataa aaagaaacgt tttggaaaat cattagcaaa taaagcacca
    18181 tcaatgtttt tgactatgtt agacaataag ttaaaatata ataatgaaag attatataag
    18241 atagatactg ctaaatgtag agccagtcaa tataatcact tttcagatga gtacaataag
    18301 aaggaattaa aagatagatg gaataatggt atggacattc aaagagattg ctattctgca
    18361 tttttaataa tgaatgttaa tgaagattta aaatcaatca acagagaatt gtgtgttaaa
    18421 acatatgata actttaaaat attacatgat aaagagataa atagattaaa aaaattaaaa
    18481 ttaaatggtt ataaattaat atctagtatg gggatataaa aactatatag gttgagaaac
    18541 gagcctaata ttaatgttaa tggtgtcaac agatactttg ttaataaaag tcttagggaa
    18601 tatgattagt gataatatgt tgtaggttat gatttattat aactgagagt attgtagaaa
    18661 ttcatgtagt acctaagaac cccacgcctt taggcgtagg agtttcagaa taacaatgaa
    18721 tggtaatata taagaataaa aagaaggata ttataagaga tgagtcgatg tatgattatg
    18781 ggatgtcttt gtcatctatt atgaaggtta tcttaaatca tcaagaagat atagaaaagt
    18841 taaaagaaga ggaggtagca gaatttataa aaaataaatt agaaaatgag catgattgtg
    18901 atgaattaaa aataataagt gaggtattta atgaaatata ttaaacatgt agataatgga
    18961 gtaatgagag caataggtat tgataattat aaacctttat taaacatcat aaaagaaaaa
    19021 gtgaatttag aatctaatta aaacagtttt aattagcgag gtgatacata tgggattaga
    19081 tttaggtttt tattcagtaa aaacaaaagt tacagattta gatatttatt taaataaaac
    19141 aagaggtgag aaatataagg aattgaataa tgatgaagaa gagttttata agtttgaaga
    19201 attattagat attgatatat ctttagaaga ttggtcagtt attcgtatga tatataaagc
    19261 caaatgggaa aaaggttatg aaagttatga agatgaagcc atacttatta caaaggaaga
    19321 tttggaagat tatataattc cttttcttaa acaaaaagat ataaattcta agatatacaa
    19381 taaggataaa gagtattatt ataaaaatga taaaaaagta acagttgatg ataggtatac
    19441 acaggagttt tgggatttag tattaaaaga atttcaaaaa atgttaaaca taattgattt
    19501 tgaaaaacat agtttaatta ttatgtattg gtattaagat aataatttta atttatttgg
    19561 aggtttataa taatgaaaaa aatgtacata gcaattaatt cagacttaaa tatgagtcca
    19621 ggcaaagttg gagcacaagt tgcacatgca gtttatgatt atttgtataa caaaatttta
    19681 gacactgttt cttgctctta tgaaacagaa ataatagcac atgaattaat agatttaaaa
    19741 tgtgatttaa cgtcatttaa gaataatggg gatacaatat gtatattaaa agctaaagag
    19801 gcacaattat taaaatttaa agaaaaaggt tatttaacta tagtggatag aggattaaca
    19861 gaaattccta aaaatagcat aacatgtgtt aacttaggaa tatttaatga ggatgaaata
    19921 ccatctacaa ttaaaagatt gagattatat taaggagaaa gacaataaat gtattctaaa
    19981 ggtgatgtag taaaagtatg gtgttatcaa tgtgaaagga tagaatttca agaatttgta
    20041 ggatatttta attcttattt tgataattat aaattaaaga aacgatggaa atgctctaag
    20101 tgtgatgtca tcaataattt ttaagagttt aattaatgga ggaatgagtt atgaaaataa
    20161 agaaaataat tttaggatta gataacttag agaatgtaga gttgggtatt ggagatgtag
    20221 gaggttttta tttagataat atacatgaaa ttattggagg atttactttg agttatatag
    20281 gtaaatgcaa acaggcagat tatttacata tacaaataca taaacgtgtt gatgaaattc
    20341 aaagattgca tgattataag gatattacaa atgtaaagat agaatacgaa gacggaagtg
    20401 tggaggatat atatgtacct tataaacaag aaggggattt cttgggttta gataatcttt
    20461 atcaaacttc ttttattagt aaacaaggtt atttacatat agttatctct aaggataaaa
    20521 cagtagaaga agtcttcaat gaagaatttg aggatgatga aggggtagaa tttagtttta
    20581 atatgtatgg aatataaaac ttaaaatgga aggagatgag caaatataat taactctttt
    20641 aagaaattaa taaagtcaaa atatataaaa aataacgaat caataaaacc attagaaaga
    20701 atttttgaat atgaaataaa aatcaaagaa aaaagaatat attttaattt agaattattg
    20761 atttatgaaa aaagaattaa gataattaaa aaaatatttt taaaagatat actatgccat
    20821 tatcgaagag atatagaaca actcgtcatt tatgacctgg ataaatatct taatatgaca
    20881 gataaagaaa tggaggaata tattataaat ttaataaaag ataaaataaa agaagagtat
    20941 ggggagaata tacttcctga aatactaatg gaaaaattta taaaactcga aagaaaaggt
    21001 tcggtgactg tagacgtacg ggatttaata gagggataat aaataacaag ttaaaataca
    21061 ggatttaata tcaatagaaa atttagtgct agttttataa taaaagtttt ggaatatgct
    21121 aaaatgaata taagtaaaag ggtgatataa ttattagttg gaggtaaata tgtgtaaatt
    21181 ttgtgacagt ataaaagata tgaaaaaaca tgaaactaga agagtaagaa tttcttctgt
    21241 aggaatcaaa ggaaaagata ctacttatga gagtacagaa cttttagacc aggaagagta
    21301 ttttgatatt agtttttata aatttaaaga agggaatata aatttatcaa tagaacacaa
    21361 tctaattgtc aaagattcaa atcaaaatat aaagttaaat gtggatagat ttgcaggagg
    21421 aataccattt aaatattgtt ttttatgtgg ggaaaaatta agtagtgaaa gtattgattt
    21481 tgagaattac atagataatt tgatgatttt agaagaatag gatttaatta aaaatgagca
    21541 cattaataat ataagtgtaa attgacttat attattaata tgaaattata atactaggag
    21601 ttatttttat agtattataa tttgaaatga aaaagtgaga aatagctatt gatggtaaat
    21661 ttgacaagaa taaatagttt agattttaca agaaaggagc ttagataaaa catgtttgat
    21721 atgataataa ataaagaaat attcaaatac aacaatgaaa atatttatga tacattgata
    21781 aatagttttt ctaacgaaac tcatgaagat gatattaaac aaggagaaac aataataatt
    21841 tttactacaa acaaacaact tgtctacaaa gaattaataa gagcagctat aaaaaataaa
    21901 tatactctaa taaagcatag gaatagaatc aaacacaata tagaaataga gttaaaagtt
    21961 cttaacacag atagattaat aaaattttta atatttgata gggatataaa ttatcaggag
    22021 ttgagagggt acaggggtga atgtgtaata gtagatgtaa acacttcaaa taaaactatc
    22081 caaaatttca ttacagagca aataaaacca gtcttacttt ttatgaatgt atataaaaaa
    22141 gaatataagc cttttcatgg agttttacta tttaaataaa attaaaaagg agaatatgat
    22201 gaatattaat actaattatg taagcacaaa tatgattgtt atggatatat ccaaaggcca
    22261 taatatagaa ataactaaag ctatggttga aaaatctata catactttta aaaatgcacc
    22321 agttatatat aatccaaatc aagaattatc agattataga gatgctgata gagtaaatga
    22381 tttttataaa gatgaagttg taggaattat aatggatgta gcaatagtag ataaccaagt
    22441 tataggaaaa atactatggt atgacaattg tcatattaaa gagaaatatg ataattggta
    22501 cattagttta agcgatgata aatccgaatt tattttaaag gaggtcgaga tatcttaaaa
    22561 gattattaaa ggagtgtaga tatgttaaaa agggttggaa ggaggataaa tggagattaa
    22621 aggaaaatac aataaagcaa aaatatttac agataatgta gatagtacaa caataagtca
    22681 aataataaat ttatgtaatc aaaaggaatt taaagaatct agtataagaa ttatgcctga
    22741 ttgtcatgct ggtaaaggat gtacaatagg aacaacaatg actataagtg ataaagtagt
    22801 gccaaatctg gtaggggtgg atataggttg tggaatggct actattccat tagaaaaata
    22861 tattaataat ataaattata caaaattaga taagattata agagattata ttcctcatgg
    22921 atttaaaata cataataagt caagagaaaa actattaaag aatttattta atatagacat
    22981 aaataatctt agatgcgagg tgaataaaaa tagagcatat atgtcgttag gaactttagg
    23041 tggaggcaat cactttatag aagttgataa aagtgaaaaa ggtcaaatgt atcttactgt
    23101 tcatacaggt tcaagaaatt taggaaaaca aatagctgat tattaccaag acaaagctat
    23161 aaaatattgt gttgaaaaat ataaaaagtc atatgatgat gcaaaagagt ttttaatatc
    23221 tactctaaaa aaagacaata aagaatattt gataaataaa tatttgaaag accttagtga
    23281 tagaaaattg aataagccac atgatgattt atgttattta gaaaatgatt taatggaaga
    23341 ttatttacat gatatgagta tagctcaaaa atacgctgct gcaaatagaa tggttataat
    23401 tgcggatata ttgtttaaat ataacagcct agatgtaaat tatgaccatt ttataagaga
    23461 tattaaatgt gatgttatag aatgtattca taactatata gatatgggta gtaaaatact
    23521 tagaaaagga gcaatttctg caaataaaga tgaaacagta ataattccaa taaacatgag
    23581 agatgggatt atattgggaa aaggtaaagg taattctgag tggaattatt cagcaccaca
    23641 tggagcagga agaattttat caagaggaaa agctaaagaa aaaatatctt taaatgaatt
    23701 tgaagaatct atgaaagaaa tatttacaac ttgtgtaagt caatcaacac tagatgaagc
    23761 accacaagca tacaaaccta tagaggacat cttaaacaat ataggagata ctattgagat
    23821 aatggaggtg ttaaagccaa tttataattt taaaagcaat tagaatcttg ttatttaaat
    23881 aaaatcaaaa ggataaaaaa agatatgtct aattaattta tgttgaattt atagataatg
    23941 aagaaaatga taattgcgat atagttagag tctaatatga agacattgag aatatagtag
    24001 aacctatagt ttcttatatg aagagtttaa atacatgtaa aatcgattta aatgatatta
    24061 caataataaa gaaatgagga caatatgaaa gagtatatat attgagtgaa aataagtgaa
    24121 ataaaagaaa tatatggtgg aggtgggaat tgtatatgac aaaaaatatt aaaatgtaat
    24181 ttaattatat aaatgattag aattaagatt ttgttatata ggaggaatta tgagattaga
    24241 tgatagtcag ataggtttaa taatgtatat gataggattt gttcattatg acaaagagca
    24301 atttcaattt tatatggatg caatgtatgg aaaggaatat tcagaaaaag aaatagtcaa
    24361 tatgttagaa catctaagac aagatattaa gattcaaaga tttaaataaa attaagaagg
    24421 agtgatatgt ataactaata tagaaattcc taaaaaagtg gattatataa ttaaagaatt
    24481 agaaaaaaat ggttatgagg catatgtggt tgggggatgt gtgagggatt gtttattaga
    24541 aagaattcca aatgactggg atataacaac tagtgctaga cctgaaaaag tagtagagat
    24601 atttaaaaag acaattccaa ctggtataca acatggtact gtaacagtca tgattgaaca
    24661 tgagccattt gaagtgacaa catatagaat agatggaagt tatagtgatg gtagacatcc
    24721 agactcaata gaatttacaa ataacatagt taatgactta tcaagaagag attttactat
    24781 taatgcaatt gcatataatg caaagacagg gttagttgac ccatttaatg gatatgagga
    24841 tatacaaaat aaatgtatac gttgtgttgg gaatccaata gacagatttg aagaagatgc
    24901 acttagaatg attagagcaa ttagattttc agctcaactc aattttagaa tgactgcata
    24961 tactatgcga ggtatatatg ataagtcaaa ccttataaaa aatatttcta tggagagaat
    25021 tcaagtagaa tttaataaaa tattactagc tgattcatca aaattaagtt cattaaaagt
    25081 attaggattg ttaagattta taattcctga aatatgtgat ttagaagata ttactcaaga
    25141 taatccatat catatttaca atgtgcaaaa acatacttta atagctacag aagtaattga
    25201 aaatgaattg catctaaagc tcacaatgtt atttcatgat ttgggcaaga aaattactaa
    25261 aacaactgat gaaaatggaa ttgaccattt ttataatcat agtagagaat ctgtaaaaat
    25321 agcaagagat attatgaaaa gactcaagta tgataatcat acaattaata aagttttgat
    25381 attaatacaa taccatgact ataatataga acccaaaagg aagataatta aaaaattatt
    25441 aaaaaaactt gaagatgttg aattatttaa agatttatta aaaattaaat gggctgatat
    25501 acttgctcaa aatccaaaat atatgaaaca gagaatatta aaccttattg attgtgaaaa
    25561 agaatttaaa tatattataa aacaaaaaga atgttttaat agaaaaaatt tagctattaa
    25621 tggtaaagat ttaatgtcta tggggattga acaaggtaaa aatataggat atgtattaaa
    25681 taaactactt gaattagtta ttgataatcc agagttaaac gaaaaagaaa ttttaaaaga
    25741 aaaagcatta gaaatattag aatcagtttg gaaagtttat gttggaggcg ttcttcattc
    25801 tgtacatcgc tttaaaataa gtgctgaaaa tagagagaaa gaattgacta aaatgggtgt
    25861 aaagggagta gtagaaattg cttatggtga tgaaatacct gatttcatga aactgtgtag
    25921 ctccattaaa aaattagaac tttatgagta ctaattataa atcttggtta taaagtgtta
    25981 gaaatttaat attaaactgg aggataaata tgagttttga agaagaggca aaaaagggtt
    26041 agagccatac aaattgaaag aaatagaata aatcaagata tagtcaaggg aaaatatgta
    26101 cataaagata aacttgctaa atgacagtat agataaaata caatatgata tagctcatgg
    26161 tttattacaa agtaaagaag aaaaatgtat tgctaaagga aagttaagta ttataaatga
    26221 aataaaagta caaatatatc tgtaaaaaaa attaatttaa aataaatatt ttaataggag
    26281 aaaaataatt atgaaattaa taaatggaaa cagaagtagt gggaaaacta ctgcattaat
    26341 aaagtatgct tatgaaaata atgcacttat attatgtcat tctattgcta ataaaaatta
    26401 cataattaat ttagcaaaaa caatgaattt agatataata aacccaagaa cctttaaaga
    26461 atacatgttt aatggaagta aaatatatga tacaaaagcc ataaatggaa tatctttaaa
    26521 aatagaccct catataaaaa tagtagtaga tgatatagat tgttgcttaa catccattct
    26581 tgggcaacat atagattgtg ctactggaac aattcaaata gaaaatctaa ataatattta
    26641 aaggagagct aaatgataaa atattgtaaa gaaaagtgga taaaaaataa aacaagatta
    26701 catgaagcat tgttagagtc tgatttcatt aatcgtgata gttactgtag ctatgaagat
    26761 ttggttaaat tatctgtaaa acatattttg aatgacaata taaatgaaga tgatgagtgg
    26821 gatgtcgaca atataagtga aatagataat ggacattatc aaggtacttt attatattta
    26881 atacctaaaa aaacatacca acctaatgaa tatgaatatc ttatgacata tgttggttat
    26941 ggaagttgta gtggctgtga tacattaata aatatacaag atagtgactc tgaagatagt
    27001 attgaagact atatgtgttt atgcaaagat ataatatgta atattataaa accatataat
    27061 tttggatgga gagaagatga taaatataat gttgtaaatg attaatcaaa atttcaattc
    27121 taagaaattc atttaaccaa ttcaaaaatt ctaaatatat attcttattt acaattcaat
    27181 taaaaataaa ggagtgaaat tatattgaaa agatttttat taataggggt attaggaaca
    27241 atgttaatag gaatgtctgg atgtacaggg atacaaagct caatgaaaga tgctgaaagt
    27301 gatgtatcag ggcttaatag aactgtaaat gtatattcag ataatggcga ggtattaaaa
    27361 acatatacaa gtaaaagcat gagagttaaa gatggagatg gtggtactat aacactagat
    27421 tttgatggta aaagggtgat tatatgtaat gctcatgtag taatagaaga aaattcatct
    27481 aataaataaa ggagagttaa atgtttaaag tcttaaaaac aattcttata ataatttggg
    27541 tagtagatat tttaaacttc ccacaatttc aattcttaga tacaacatat ccaatcaata
    27601 ccttagcatg gttgctaatc tggattctta taccatcaag tagtatatat attgacaaaa
    27661 aagaataaaa gaaaggagta ttaatttgat tttaaatatt gctgcaattt ttaatttagg
    27721 ttgtatggta gctaattact tgcaagataa tactaatggg atactactta atggattttg
    27781 tcttatagca ttattattat tgaataataa cttagattaa aagtattatt ttaaggagat
    27841 acatatatga gaaaagtaaa tgtattagaa aacgaaaaat ttgagataaa aaattataac
    27901 attatatgtg ctatatcaat gaatcacagt aaagatgtaa atcaattcga ccaatataga
    27961 ctatatgatg ctcaatataa tttagtagag atagatgaga agttaaaaga atatggatat
    28021 attttaatcc atacatatca aaatggatgg gattcgtggg gcgacgaata tgtttgtact
    28081 gtagaagata ttaaaaacat aataaaaaat gctaaaaata aagagcattt atttataaag
    28141 gcactagaag aatattttgg aaaacaagat taaaatattg aggatatact tatatttgtt
    28201 gttgctacta taaatgaaat attagtaaac aaaacaatag ataaagtggt aaataagtag
    28261 aggtgatatt tataagtagt acaaatagaa gtaatgctag agattttcat gtaagtgatt
    28321 attatagaac tccaattact aaaatcaaag agtttttaac tgagttcagt agatatgaaa
    28381 atatactcaa ttcaaatata aaaatattag acccatgtgc aggtggagat aatacaaatc
    28441 taatgagcta tccaactgca attcaagaat tttcaaatca agaaatttca atagatacag
    28501 tagatataag agatgattca agagcaaata taaagcaaga ttatttaaag tttcagccaa
    28561 aagaaaatta cgatgtaatt attactaatc ctccctttaa tttaagcttg gatataatta
    28621 ataaggcttt aagtgatgtt agagaaggtg gttttgttat tatgttgcta agattgaatt
    28681 atctaggagg aaaaataagg caagagttgt ggcgaaataa tatgcctaag tatgtttttg
    28741 ttcacagcaa aagaatgagt tttacagatg ataaaaagac agatagcata gaatatgctc
    28801 attttgtatg gcagaaggga tataatccaa aattttctca gttaaaagta ttaataagtc
    28861 aatgaaatag aggtgtaaag tgaaagttaa atacgttgta tttgaatggg agattacatc
    28921 aaaaaatgat ggccaaaaac attttataaa ttttagagat ttaattaaat tatatggagt
    28981 gtcgccaaga gaatgtataa gagcaaagga ttattacgag agagatggtt tagaccttaa
    29041 agatataaaa tttctatatc caagggatga tggaaaatat aaactataaa atcatggttt
    29101 taaatgatta taaaatgtca gttttaaagg gggctaatca tatatggata ttaagataga
    29161 tatgtgttta ttagagatgg aaataaatag aagaagatgt gagtatgctg ccagaacagg
    29221 aagatatcct aattctctta ttttgtggga aggttactat agccttctta atatccaata
    29281 tatagaggat aaaacaagag gatatattac taagtttaat ggcatggatg ttataaaatg
    29341 taatgacttt ttaaaaatag aattatttga aaagaatatt aataattaaa atttgttttt
    29401 taagttgagg tgatgagttg agatataata attatcatag tcataaaatt tattcaaata
    29461 taaggtcgtt agatgtaatt actaaaccac aacaatatat agatagagca ataaaactag
    29521 gtcaaaatac atattttacg acagaacatg gatatcaagg aaatgtatat gaggcaaaaa
    29581 ctttatgtga tgaatataaa ttaaaaatga ttattggagc agaattttac tatgtaaatg
    29641 atataaatga gaaagataga ggaaattatc atttaatagt aatcgctaaa aataatgatg
    29701 gttacaagca aataaataaa gctttaagtt tagctaataa aaatggatat tactataaac
    29761 caagaataga tgaaaaaata ttatttgaga tatttaaacc aggggatgta gttattacta
    29821 ccgcatgtgt agctggaata ttaaaacttg agaatagaga agaattaata ctaaaactaa
    29881 agaattattt taaaaataat ttctttcttg aagtacaatc tcatcctcat aaaactcaag
    29941 ctttgcacaa taaggatgtt ttagaattaa gcaacaaata taatatagat ataatacacg
    30001 ccaatgacag tcattatatt tatccagaag aatcaaaata tagaactaaa tttcttaaag
    30061 ctaaaggtat aaattatcct gaagaagacg gttttatttt agattatcca aattcagagg
    30121 atatatttaa aagatatgaa aaacaaaatg tattaacaaa gcaagaagtt gaaagagcat
    30181 taaaaaatac tctaatattt gatgagtgtg aagaaatcac cttaataaat gatgatataa
    30241 aattaccatc aatatctaaa aatccaaata aagaactgaa agaaatatta aataaagaat
    30301 ggttagagaa aagaaaaaat atacctaaaa atagatggaa tgaatactta gatgctataa
    30361 ggtatgaatt tgatattata gagaaaactc atatggagga ttactttatt atagattata
    30421 aaatagttca aagagcaaaa caagaatata atggtcttct tactaaaaca gggagaggtt
    30481 cagcaccttc atttataata actaatcttt tagggcttac tgaaatagat agattaaatg
    30541 ctccggtacc attattccct actagattta tgagtgttga aagaatacta tcagcaaaat
    30601 ctttgccaga tatagattta aatgctgaag atgctgaacc atttatacaa gcaactagag
    30661 atttattagg agaagaaaat tgtgcatgga tgataagtta taagccatta caagatgctt
    30721 cagcttttag gctatggtgt aaagcaaatg atatgaaact atctgagtat gatgaagtag
    30781 ctaaaaactt agataaatac acaaatgatg ttttttggaa agatgtaata aaggaatcaa
    30841 aggtatttgt aggagtagta gaaagtgttt ctttctcacc atgctctatg cttatatatg
    30901 atagaccaat tgatgaagaa gtaggattat taaaaactaa ggatggggta tgttgtaata
    30961 tagatggata ttattgtgat aagtataaat atttaaaaaa tgattattta acagtgaaag
    31021 tatggtcatt aataagaaaa atttgtgaat tagcaaatat tagtattcct acaattgaag
    31081 aactaaacat cttattagat gctaaaacct atgacatata taaggacaaa ttaacttgca
    31141 ctataaatca ggtagattca gactatgcta caaacctggc atctaaatac aaaatatcta
    31201 gtgtggcgga aacaagtgca tttgtagcta gtatcagacc aggatttgca tctttattag
    31261 acaattttat taacagagag tcatatacaa ctaacgttaa agaactagat atattattag
    31321 aagatagtta tcattatctt atgtatcaag aatcaataat gaaatattta atatggttag
    31381 gaatagagga atctgagagt tatgatatta taaataagat taaaaaaaag agatttaagg
    31441 aaaaagagtt gaaagaattg catatcaaac taaaaaataa ctggattaaa aaagtcggaa
    31501 ctgaaaataa ttttgaagat acatggcaag ttgtaaatga tgctgcctca tattccttta
    31561 atgcttcaca tagtctttca tatgcatatg acagcttata ctgtgcatac cttaagtcac
    31621 attatccatt ggaatattat actgtagcat tcaatttata taatgaagat acagagagaa
    31681 caagaaaatt aacagatgaa atagagtatt ttagtattaa attagaaaat cctaaattta
    31741 gattttcaaa atctgaatac ttctttaata gagacacaaa tagtatttac aagggcattg
    31801 aatctattaa atttttaaat tctgatatag gagagtatct atattctctt aaagataata
    31861 aatatgattc atttttagag ctattaatag atttgcaggg gcatataaac tctaaacaat
    31921 tatctatact gattaagcta gatttctttg aggaatttgg aaagtccaac aaacttttag
    31981 agacttatga tatctataac tcaatctata gtaaaaaaca atttaaaaga gataatttac
    32041 catgcaatat aaataccatg agaaaatact ctaataaaga aacagaaaaa atatttaaag
    32101 aagttgatac aaaagaatta tgttcttatc ttgaaagtca aattgaaaat acagatatac
    32161 caaccaatga aaaaatacaa gctcattttg agtttgtggg ttcatgtaac ataaaagata
    32221 gcaattcaaa tccaagatat tgtttagtaa tagatataga tactaaatat tctcctaaag
    32281 tcacattgta taatataagt tcagggaata taaagatttt caaagtaagt aaacctattt
    32341 ttgaggataa taaaattgat gtttatgatt tagtttattt aaaatcaatt aaagaaaaag
    32401 ctaaaagtaa aaaagtagat ggaaaatgga ttaggagcaa tacagaaaca gaatggtgga
    32461 tacaagaata ttggaaaata gaaaattaag aatatgacct cgtatatgcg tttctaagac
    32521 tttacataag agtctgcttg tgtttataca ttgatttctt aaaatggcat atacgaagtt
    32581 cgtgaaaggt agtgatttca atacttatag atgaacaaag atattatagc ttattggaag
    32641 aaaaaaaaga cctaagtatg agaatcgatg aattaaaaga ggaaaatgct tttttaaaac
    32701 aagaaaatga agagtatgaa gaaattctta atgagtggga atgtgtaaga aaagaaattg
    32761 tagcaacttg taaaaaaaaa gaaatattag gtgaaaaacc aagtgaatta gaaatggttc
    32821 ttcaaaaaat agaggatata taatgagtgt aaataaaatt tatgagaaaa aagaatacat
    32881 tattatcaaa tcaaaatctg gatatatagt ttataataca aataaagagt ttaaagaagg
    32941 ccacactcac ttaaaaagtt ataatgctgc taaaacagct atagatttag ttattaagag
    33001 aaggcttcct aaaagttatt ctatttattt tcttacaagt ttgatgagaa tatctaatga
    33061 tgtacaata
//
LOCUS       DAMJYR010000033        32660 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.033, whole genome shotgun sequence.
ACCESSION   DAMJYR010000033 DAMJYR010000000
VERSION     DAMJYR010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 32660)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32660)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32660
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.033"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            321..1154
                     /locus_tag="PC471_001547"
     CDS             321..1154
                     /locus_tag="PC471_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase"
                     /protein_id="HDF2788679.1"
                     /translation="MRNKKKKINRKKLYLLLSAIVVCLAFIVVGVRVSSLNMKENEIS
                     RNAKLSSSTITDIVSNTSIEVSKGPSKSSGKIAYITIDDGPSKFTDQMIKTLNKYNVK
                     ATFFMIDGNMKEYPQQVKNIIKNGNTAGFHSVSHDIHKLYVTSTSAKEEFDTNDQTFY
                     KITGKHSKVIRIPYGSKPYTPQASYQALVDAGYKIWDWDLDTEDWRSNSSQIVQNVKN
                     HIKNRKGEDKDQLVVLMHEKKQSAEALDSVLKFLSDEGYEFAPVDQNQIPKNYWLRNL
                     E"
     gene            1247..2179
                     /locus_tag="PC471_001548"
     CDS             1247..2179
                     /locus_tag="PC471_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HDF2788680.1"
                     /translation="MVGILSLMMVGCNKSLAKVNDVEITKEQYKKTKAVLSATNNYIN
                     GQSLDELEKTLDKKGRNKLENVIISFMVDNELLYQEAKDKGLTPSKSEVDSKYQELED
                     KMNLNTSYKEKMDKAGVDKDYLKQEISRDLAIDKNKKAFEDRINISDNDMEAYYTSHK
                     KDFNVEEVSASQILISTLDKNKKEVSKDKKEALKKKADNILTKIKNGESFESLAKKYS
                     DDKATGKNGGQLGYFTKDDKNAEFTKEVFKLKKNEVSNVFETSYGYHIVKVTDKRERQ
                     KSFNECQSLIRESILNEKYIEHIKKLNEDAKIDR"
     gene            2231..2713
                     /locus_tag="PC471_001549"
     CDS             2231..2713
                     /locus_tag="PC471_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HDF2788681.1"
                     /translation="MFKSLKRTKVEKYIIDNKDSFYRIAYSYTKNEEDALDVVQEAMY
                     KALYSVENIKEVNYIKTWFYKILVRTSIDFIRKNRKYNNMTDIDLIDETGEYDKYTDL
                     DLRRALEELPIEYKSIIILRFFEDLKIEEVAIILDENVNTVKTRLYTALKKLKLKIEE
                     "
     gene            2725..3594
                     /locus_tag="PC471_001550"
     CDS             2725..3594
                     /locus_tag="PC471_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 domain-containing protein"
                     /protein_id="HDF2788682.1"
                     /translation="MTSKERLNKLKDEYHKIPIPKKLDTIINHEKIENIYREKNKKVN
                     RLRFKVAIAFACIFTVLVNISPVFADNFSKIPVIGAIVEVITIKNYSLKSENYEAEID
                     IPKIRGLKDKNLEQRLNSSFMEDGKRLYHQFQERMEKIQSSKNKGYKSLSLSYSVKNN
                     SKKFLSIEMTKNEIEASSYVSKVHYTIDKKRQIVLTLPMLFKDDKYIKVISDNIKEQM
                     REQMKKDSTKSYFIDQKKDLPVEDFKTINKYQDFYFNKNEDLVICFDEYEVAPGYMGA
                     VEFVIPYKVIKDL"
     gene            3637..4392
                     /locus_tag="PC471_001551"
     CDS             3637..4392
                     /locus_tag="PC471_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 domain-containing protein"
                     /protein_id="HDF2788683.1"
                     /translation="MTTYKKLKKSIFKIAIFSICLLASLVDTNPIFAGSSFNETSNSD
                     TIKVLNAKSINFKQDNYEVNVSIPKIKGLKDKTLENKLNNEFLQDGKKIYSEFQNKMK
                     KEDNKGHRYLNVIHNVKNNSKDFLSIEMTKEEIEASSSITKKHYTVDKNRGILLNLPI
                     FFKDSRYIRAISDNIKEQMRNQIKKDDTKSYFIDQDKDSPVKDFESINKYQDFYFNKN
                     GNLVISFDEYEVAPGYMGAVEFVIPNKVIKRLR"
     gene            4434..5255
                     /locus_tag="PC471_001552"
     CDS             4434..5255
                     /locus_tag="PC471_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase accessory factor"
                     /protein_id="HDF2788684.1"
                     /translation="MNIYEQAIRLIENNEDFAFATITSHSGSTPRETGAMMIVKNDST
                     IFGSVGGGSVEAECIKHAINVIKNRESMLYKFTLNKSDVAKLGMICGGTGEIQIDFID
                     SKLKSNIEKFNKRLKENTSKAYIFGAGHISRDVAVILSLLEFRTVVIDDREEFANHER
                     FPESEVVVLDSFENIPDFPTDENSYIIILTRGHLYDSSALEWALKREAGYIGMIGSRT
                     KIGLTYEKLMKKGFKKEELSKVHAPIGIKLNAQTPAEIAVCIAAELINCRANKEK"
     gene            5260..6021
                     /locus_tag="PC471_001553"
     CDS             5260..6021
                     /locus_tag="PC471_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HDF2788685.1"
                     /translation="MNTGSKDIDTLKVNFRGIEIKAVLVYKNRKNISIKIDPFGKITV
                     MSPPKISKKIIKDIIIEKGDWILKKSEKCKGREEVYKQRMFITGEKFLYLGEEYHLVI
                     KELLRDSVKKSNCRITINEYQIVVQTNDTSTDFIKKSLKSWYKMESERIVLERIDFLK
                     LNCEIMKQLVPASVKVKEQNKRWGSCTAQKNIYINSKISMARLDVIDYVIIHEFSHLV
                     HMNHSKKFYDLVKSIMPDYKDKENWLKINGYKIII"
     misc_binding    6182..6439
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            6552..6791
                     /locus_tag="PC471_001554"
     CDS             6552..6791
                     /locus_tag="PC471_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HDF2788686.1"
                     /translation="MENTVYAKLNQGIDSFLRVAMTLRRREVDIQSISMTVDNMNNSG
                     IKLIVNEEKTSLDSVLNHMKKLHDIREITVERMRH"
     gene            7095..8090
                     /gene="ilvC"
                     /locus_tag="PC471_001555"
     CDS             7095..8090
                     /gene="ilvC"
                     /locus_tag="PC471_001555"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HDF2788687.1"
                     /translation="MKMARMYYEKDVDLEVLKNKKVAVLGYGSQGHAHAQNLRDNGVH
                     VVIGLYDGSKSAQKAKEDGFEVKSVAEATKESDLTMMLMPDEKQKKVYEESVKDNLKE
                     GQTLAFAHGFNIHYNQVQPPEFVDVVMVAPKGPGHLVRNVFTKGSGVPALFAVYQDYT
                     KKATETVLAYAKGIGATRAGVLETTFKEETETDLFGEQSVLCGGISELIKLGYKTLVD
                     AGYQKEVAYFECLHEMKLIVDLIYEGGFERMRYSISDTAEYGDYVSGKRVITDVAKQG
                     MQNVLEDIQNGKFAKAWIKENEEGRENFLKTREEEYNTEIAEVGRNLRSMMSFLK"
     gene            8143..9831
                     /gene="ilvB"
                     /locus_tag="PC471_001556"
     CDS             8143..9831
                     /gene="ilvB"
                     /locus_tag="PC471_001556"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HDF2788688.1"
                     /translation="MNGAKVILECLKKEGIDTIFGYPGGAVIPLYDALYDYSDDFKHI
                     RTSHEQGLVHAADGYARSTNTVGVCFTTSGPGATNAITGIATAFMDSSPMVVISGQVP
                     TSLLGKDSFQEIDITGATLSMTKHNYLVRNTKELVPTIKEAFRVANSGRKGPVLVDVP
                     KDLFLAEMDFSGEDYDLCQIDDYMDYKSDFDLDDETNIKLLNEAIDIIKESKKPIIYA
                     GGGVKSSDSEEILEKFATKIDTPVLNTLMGLGNIDRKNELSLGMVGMHGSRESNLALS
                     NSDLVIAIGARFSDRVISKSSEFAKNAKIIHIDIDPSEISKNIESNVSLVGDVKLVLS
                     LLIERVESKNNSNWKEEIKRFRKSEGVQTYEFHPQNILKKINEKYETLKKPTVVVTDV
                     GQHQMWAAKYWNFKGNKSFITSAGLGTMGFGLGAAIGTKVGNVDKNVVLVTGDGSFRM
                     NCNELATVANYNVPMLILLLNNRTLGMVRQWQKLFSNQRYSQTDINENVDYVKLVNAY
                     NIDGYKVSSMEELGKALDMIDFNKPVFLQCDIDKDYDVYPIVAPNDALENLICN"
     gene            complement(10192..11322)
                     /locus_tag="PC471_001557"
     CDS             complement(10192..11322)
                     /locus_tag="PC471_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDF2788689.1"
                     /translation="MKGGVRKRSNKWYYYFDLGIVEGKRKKVERVGGNTKKEAEKALR
                     EALNEYENSGIVFEESNISLSDYLDFWYKEYVLLNCKYNTQESYRINIEKHIKPKLGA
                     YKVKALTPAILQNFINKKYKEDYSQNTLQVLKAILHRSLKSAVHPYKHIRENPMQYVS
                     IPKTKSKTETNKVKTITVEEFNQILNIFPQDSFQRIVLLIGFHTGMRRGEIIALKWDN
                     IDLNNKTITVRHTLIKKPNGMFELGQPKTESSCRTIFTGDTLIKALKEHKLYQKKMKL
                     KYGEFYFDSDWVCTKENGQQVNTHTLDTIVRQIRVALNNDFHFHSLRHAHATLLLENG
                     ANIKDIQNRLGHSQLSTTMDTYSHVTDKMKNETVDIFEKITN"
     gene            complement(11562..12068)
                     /locus_tag="PC471_001558"
     CDS             complement(11562..12068)
                     /locus_tag="PC471_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HDF2788690.1"
                     /translation="MNKLDALLDLANNEEIEIYYTDKIADDIKGLYINRQGLKIISLL
                     NSLKQNNAKLIEILAEELGHHFTSVGNYVSSKNSYKNKILIDKTENKALKWACEFLIT
                     EEEIIHVINSHATSVYEIAEELQVSINFLLKRLEFLSKKKSMLDLGNNRFLVLTNLPN
                     FYIYEDIF"
     gene            complement(12331..13920)
                     /locus_tag="PC471_001559"
     CDS             complement(12331..13920)
                     /locus_tag="PC471_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIR2 family protein"
                     /protein_id="HDF2788691.1"
                     /translation="MKSIISDVINRFNATPFLFVGSGLTRRYYNLPNWEDLLKVFAEK
                     ISNDDFIYTSYKNKAKSENPKMGINPRIAELIENDFNKKWFSDSSIRSLDSKYLDIVK
                     SGVSPFKAEIAMYIKNNSKIVDKYQDEVEKLSNISKKSLSGFITTNYDCFLETIVDNY
                     TTYIGQENLVFSSIQGIAEIYKIHGCVSSPNSIVINEADYIDFDSKSAYLAAKLMTIF
                     VEFPIIFIGYSVTDVNIKKILNAIVNCLSNENAKKLEERFIFIEYEKNFNDIEISSHT
                     IAFDNKMITMTKIKLEDFNLLYEALSEKKSKLPVRILRMFKQEFYDFTITNKPTAKIR
                     VGSVDDTRIKDEDLVLAVGKASDFGLKGLKGLSFDEWYRDIVMNDLEFSSDELLEYAY
                     PSLIKQYNKLPLNKHLFNSTLEFPDYRTIALESDFENIISNSIKKNRNNTYIKNRSVY
                     GIWNDEHQSFEKATRLIAFLEEKEIDTEQLENLLKKIFEENPNILESAKTSEKTNLRR
                     LIRIYDYMKYSNMQKSLGYSE"
     gene            complement(13944..14507)
                     /locus_tag="PC471_001560"
     CDS             complement(13944..14507)
                     /locus_tag="PC471_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HDF2788692.1"
                     /translation="MSILSDRLKFLRKEKGVMQKEIANYLNITTSAYGFYEQGKRTPT
                     PEMLSSLAEYFGTTVDYLIGRYDNKASNISSKTSCNNTLFQKRLKELRAEKNMTQEDV
                     ANKLNLTKSAYGYYEQGKTVPDAYMLSSLAEIFNVTTDYLLGRSIVKNDIDTVAAHRV
                     NPHKDLPEEAQEQLNDYIEFLINKYKK"
     gene            14660..14881
                     /locus_tag="PC471_001561"
     CDS             14660..14881
                     /locus_tag="PC471_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2788693.1"
                     /translation="MNNLKELRTERNLKQKDVALKVGITTSYYGMIESGTRVPSLAIA
                     IKLSKCFGVSIEKIFLNTNTTRCCLKERS"
     gene            14884..15699
                     /locus_tag="PC471_001562"
     CDS             14884..15699
                     /locus_tag="PC471_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein/antirepressor Ant"
                     /protein_id="HDF2788694.1"
                     /translation="MKDLKIVKVNNKLTTDSRDIALMVEKEHKILLRDIRNYINQMEE
                     ANKNMSTDLYPSDYFIENTYLDDYKREKPCYAITKIGCDFIANKMTGIKGTAFTGIYT
                     KKFEEMEQVLKNEQPKLPTTYKEALQHLIEQVEVNEKLQLEGKMKDQVIKELKPKADY
                     TDMILKNKGLVTITQIAKDYGMSGKEMNKILHERGIQYKQSGQWLLYKQHQGKGYTHS
                     ETIDITRSNGMPDVKMTTKWTQKGRLFLYDLLKTNNILPDIEKEYSYQTSILG"
     gene            15746..15955
                     /locus_tag="PC471_001563"
     CDS             15746..15955
                     /locus_tag="PC471_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2788695.1"
                     /translation="MENKKIEEIEKRITMLENQLQAQLFSQITAQSNSDFIDYMYQCI
                     VLYLHEMSKSEKRKISEITKERIRK"
     gene            complement(15965..16339)
                     /locus_tag="PC471_001564"
     CDS             complement(15965..16339)
                     /locus_tag="PC471_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2788696.1"
                     /translation="MQFSNNISKELQESIRKTVSDTSNTEYFYYAEFQYKIILKSIEE
                     FEKELDDEHEIALKLTNFGKDVLMIVEEVGYHNPCLIHYYGIVNGVYSEILQHTSQIN
                     FMITSVKKTDPSKPARRIGFIL"
     gene            16415..16612
                     /locus_tag="PC471_001565"
     CDS             16415..16612
                     /locus_tag="PC471_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2788697.1"
                     /translation="MLIGDNIKQILRKRDIKPYKLAKELDIDVSGLYKLLRNKSSNPT
                     IDTLIKLADYLDITLDELVGR"
     gene            16643..17035
                     /locus_tag="PC471_001566"
     CDS             16643..17035
                     /locus_tag="PC471_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF2788698.1"
                     /translation="MDISESITKQFSDSLKSLIEIEISKQETDRVKSQTIEQKVKVLE
                     PKDIVVLIKRGYPNYLITVEEARGILKLDTAFMRRLVSTGLIKSLVRGDGRKISRYEV
                     DDFIERNQGKNLDELLKAVERGDEIAES"
     gene            17016..17165
                     /locus_tag="PC471_001567"
     CDS             17016..17165
                     /locus_tag="PC471_001567"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2788699.1"
                     /translation="MKLLSLDTNNKNVVTLKKDGKVIADIVFKDIKTGKKISVGILNK
                     KVLVK"
     gene            17236..17673
                     /locus_tag="PC471_001568"
     CDS             17236..17673
                     /locus_tag="PC471_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2788700.1"
                     /translation="MVEVNVNVKVKVEAPEFTNALLVVANALGGLNLGKAMQIEPINI
                     TDMKEEKKVEVKKAEKIKKVEVKEDVKEEIAEAKEEIEKNNENTTSEVKYTKEEVRTK
                     AAQVSKAGKKDKLKELFGEFGASKLSEVKEEDYSAFMNKLESL"
     gene            17685..18851
                     /locus_tag="PC471_001569"
     CDS             17685..18851
                     /locus_tag="PC471_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2800 domain-containing protein"
                     /protein_id="HDF2788701.1"
                     /translation="MPLQHARLSASGAHRWLHCTPSIKLEENYPPSTSIYAEEGTVAH
                     ELAEVKLMLEYEKISKKAYNARIKKIQKSEYYNSEMEDYIQSYVENVVELVNDSKAIC
                     DDVIVMLEERLDFSEWVPEGFGTGDVVVISDGILQVIDLKYGKGLEVSAIENPQLRLY
                     GLGAYNQFEMLYDIDLIKTTIIQPRLDNISSEEIEVTKLLTWADNVKKKAQMAFNGEG
                     EFVSGSHCGFCRAKNDCRKRAEDNLKLARKYDFADTFALNKYEIADILGFAKNIQDWL
                     KDVQSYALEQAEKHGVKYPGYKLVEGRSNRKYVDEQEVAKVLLNSDYDEEKIYKPRTL
                     KGISDMEKAIGKKSFAKLLSDLIIKPVGKATLVVESDKRSEINSIDSAKKDFEI"
     gene            18866..19201
                     /locus_tag="PC471_001570"
     CDS             18866..19201
                     /locus_tag="PC471_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2788702.1"
                     /translation="MPLDAGIKDKLDESISIILDLKDRKKSIEKDLDYEKDNLLDMLK
                     ILNITEYNSTEENAKVIVMDFKRESLIKDKVMSTFLEVNDNHLDRVYIPEHIKVSPVC
                     FVSVRAKDN"
     gene            19254..19826
                     /locus_tag="PC471_001571"
     CDS             19254..19826
                     /locus_tag="PC471_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase subunit sigma-70"
                     /protein_id="HDF2788703.1"
                     /translation="MSNQEITKLVENNLNLVHFSINRFFKSYVEQHPYLYEEFYQEGC
                     IGLYKAALNYDSNRGKFSTVAVSYIRIEMFKALRYGEKHYRNSIEGLDVSVFEDSKRP
                     LKDIHGFYEIQFNYDVDYIRKIARKSYLKDIDKIVDWILQDKKISDIGELLNVSSTVI
                     CGRIKLFSKQVRDLDKFGEITSNIKSIYQI"
     gene            19849..20421
                     /locus_tag="PC471_001572"
     CDS             19849..20421
                     /locus_tag="PC471_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2815 family protein"
                     /protein_id="HDF2788704.1"
                     /translation="MSNSVQSTKVVTGKVRLSYCNIFKSRAMVEGAEPKYSVCILIPK
                     SDKVTLGRIKKAIDAAKEQGKTSKWGGKLPGNLKTPLRDGDAERADEAEEYVGMYFLN
                     ANSTQKPGIVDKDLNEILDNTEVYSGCYGRVSINFFPYNSAGNKGIGCGLQNVQKLAD
                     GEVLGGARASAEADFSDDFEYEDEEEDFLS"
     gene            20421..22379
                     /locus_tag="PC471_001573"
     CDS             20421..22379
                     /locus_tag="PC471_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HDF2788705.1"
                     /translation="MRTLSIDIETYSDLDIKKVGVYRYVDSANFEILLFAYAFDNEEV
                     KVIDLVNDEELPKEVIEALNDNKVIKSAFNANFERTSISKFLNINLKPNEWSCTMIKA
                     LTLGLPGSLDSVSKALKFNEDKQKMKEGKALIQYFCKPCKATKVNKGRTRNLPIHDME
                     KWNKFKEYCKQDVVVEREIRNKLSKYKTTEREIKLWYLDQRINDTGIKVDTELIENAI
                     ECDKRYTEKLTKEAIKITGLNNPNSPAQLKKWLSDKVGFEITSLTKESIPEILKQVDD
                     ENVVRILELRKLMSKTSIKKYEAMKLAKGNDNRVRGLLQFYGANRTGRWAGRLVQVQN
                     LPQNHIEDLDLARNLLKEGDFDLIELLYDSVPDVLSQLIRTAFIPSEGHRFIVSDFSA
                     IEARVIAWLAGEKWRLDVFNSHGKIYEASASQMFKIPIENIKKGSELRQKGKLAELSN
                     GYGGSVGALISMGAIKMGLKEEELQPIVTAWRNANPNITKFWWDVDKAAKKAIKDRTI
                     VEIQHGIKFIYNPGVLFIELPSSRRLSYLRPKIEPHTTFSGDKITYEGMEQTSKQWKR
                     IDTYGPKLVENIVQATARDCLREAMFNVTDAGYSIVMHVHDELVIDVDKKGGSLEEVN
                     SIMGKEISWAKGLPLKADGYECDYYKKD"
     gene            22391..24808
                     /locus_tag="PC471_001574"
     CDS             22391..24808
                     /locus_tag="PC471_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2788706.1"
                     /translation="MDIKASEIEHINVRHDGQLMLAIGKNKLETHWKNKSILWSELVN
                     RLSKTLRTQETYTEYRKMSKTEKDRVKDVGGFVGGSLKNGRRKAENIANRSIITLDID
                     YANKDIWEDITLLNDYACLMYSTHSHTENNPRYRLVIPLTRPVLPEEYQAISRMIADT
                     IGIDMFDDTTYQPHRLMYFPSTSIDGDYIFKFQDGEFLNPNEILDLYLDWTDVSYWPE
                     SSRERQKFNTQLKKQQDPIEKAGIIGAFCRSYSIKETIETFLNEVYIPGIDETRYTYS
                     EGSTSGGVVIYDDKFSYSHHGTDPASGILCNAFDLVRIHKFGELDEDAKPETPVNRLP
                     SFTRMSEFASSDTKVRKTIGRENLDKAKDDFGDIDFEDDEWLTRLDYDNKGSYKKTTN
                     NILMFIENDPYLKGKIAYNEFSNRAVVLGKLPWRKDDKLNDWNDSDDSGLRHHIETIY
                     NISSPSKVNDALIIAFENNTFHPIKDYLNSLKWDGIKRVDTLLIDYLGAEDNHYTRTI
                     IRKVLVAAVARVFNPGIKFDNMMVLSGPQGMGKSTFIKKLGGDWYSDSLTTVQGKEAY
                     EQLQGVWLLEMGEMMATKKADIEAVKHFLSKSEDIYRVAYGKRTSRFLRQCVVIGTTN
                     DKEFLRDKTGNRRFWPIDAGVKKTNKSIFNGQLDNERNQIWAEAVELYKANEQLYLSD
                     EEKKEAERQQRTHSEENAKSGIIEEYLNKPITKNWYDLSISEKREYIHGSDFGDLKEG
                     TILREKTCVMEIWVELFNGEPKQLTPILSREINDILKGLDDWISYNGNLRFGKIYGRQ
                     RAYVRKS"
     gene            25090..25416
                     /locus_tag="PC471_001575"
     CDS             25090..25416
                     /locus_tag="PC471_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VRR-NUC domain-containing protein"
                     /protein_id="HDF2788707.1"
                     /translation="MLESKIEKRLKKEIELLGGKAMKFISPGEAGVPDRIVLLPEGHV
                     IFVELKAPGKKLRKLQQYKMRELRELGFKVKCVSTLKEIDDFIKEVKGWNLNHIHTKN
                     TQLEKL"
     gene            25365..26735
                     /locus_tag="PC471_001576"
     CDS             25365..26735
                     /locus_tag="PC471_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HDF2788708.1"
                     /translation="MEFKPHPYQEYTIRKTIDNNNIGLLLDMGLGKTVCALTAISELM
                     YDYFDISKVLVIAPLRVARDTWSSEVKKWEHLKHLKVSKVLGSKLDRVRALSTDSDIY
                     IINREMVPWIVDFYKRKWPFDMVVIDELSSFKSNKAQRFKSLKKVLPLTKRVVGLTGT
                     PTPNSLIDLWAQMYLLDRGERLGKTITGYKERYFEPGQKNYQTGAIYNWQPKDGAENA
                     IHNKIKDICISLKAEDYLNMPKKIDNKIEIHLDSKILKYYKELEKEKILELEKDIITA
                     SSAAVAANKLLQLANGAIYDNDKNVKELHREKLEALKEIIDVSNGKPIIVFYNYKHDY
                     NRLMKEFKSLKPRTIENSKDIYDWNNGRIQLLLCHPASTGHGLNLQSGGSIIVWFGLT
                     WSLELYQQANARLYRQGQRETVIIHHLICKGTIDEQVMEALENKDKGQSALLEAVKAK
                     LKEYRE"
     gene            26759..26896
                     /locus_tag="PC471_001577"
     CDS             26759..26896
                     /locus_tag="PC471_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2788709.1"
                     /translation="MELISPGKIVRCAWCGRRFYKLDKSKVKYCSRSCSKRARRSDLS
                     G"
     gene            26889..27368
                     /locus_tag="PC471_001578"
     CDS             26889..27368
                     /locus_tag="PC471_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 region 4 domain-containing protein"
                     /protein_id="HDF2788710.1"
                     /translation="MDKESIKEIVRELRKEEREDRKKGVLYNTRLLMKHYNDFKRHID
                     SAVSEAKDVDYLEDDLGKLDNEELYILSIKKSKARTIIMIAHIDSAMKTLKLRQERLH
                     SYEKYRALELYYCDEKTYEEIAEILNCGVVTSRRWINEMIKELGVYLFGIDGLKLTV"
     gene            27853..28089
                     /locus_tag="PC471_001579"
     CDS             27853..28089
                     /locus_tag="PC471_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2788711.1"
                     /translation="MPNFGTTFVTEEAVYNMSFRSNKAEAKLFTKWVTKALKQIRIHG
                     YYIATEKDQEWLDIRTEGKKVRKDFTDEIQEFVY"
     gene            28392..29366
                     /locus_tag="PC471_001580"
     CDS             28392..29366
                     /locus_tag="PC471_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2788712.1"
                     /translation="MSNFKKRYIDVYRIDIINKINGSVYSDEEILKEVIKTFFKDKEY
                     CKENKNGINITKDKKFWITEVKCSEDDLIIKVKLEYTKYNQNTNIINAHTKEVEGNKK
                     MDQGDSNKQHLFIKFMKENNIAVVLFERVFVGVPMIELSKNLHLYYEDMIENNKFDNN
                     MSKTSINITQIASKDFIEQILDLDGVSKITLNVDREKFGTDEDNLFSNLNNSRRHNDL
                     IYKPTFRTRYLPSEVKRCCEKYIKGEEFRNKKINRIIIEGEKNKRKLKLDTEGIKLCR
                     EIEVSLDIDNHIDSEDIFDKMNNLIDDLIENNSVFFNLMYQEVAMSEA"
     gene            29387..29896
                     /locus_tag="PC471_001581"
     CDS             29387..29896
                     /locus_tag="PC471_001581"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2788713.1"
                     /translation="MYKKVLNILLNYYKAINSKEVVELVLTPVLICIISYMFFNSSIN
                     KEVVISLNKDVLTLSGLLVAFGVCIITLLFTTYNKSISEAKDIKTERKVNGFNISYFQ
                     FIQLKAYYTVIMEILVVVICFINTIMLTRYYSNIIFYVLIFFTVHIILSLTTLIISMF
                     HLSWKDRGD"
     gene            29966..30658
                     /locus_tag="PC471_001582"
     CDS             29966..30658
                     /locus_tag="PC471_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2788714.1"
                     /translation="MQDVKEKVKQDYLKGMKQKEISSKYDISLNTLKSWIKRYNWASE
                     KKKGAPINKRGAPFSNKNSVGHGAPKENKNAEKFGFFSKYLPKETRELIQEISIKDKF
                     DILWEQITIQYAAIIRAQKIMYVKGKEEMVKELKKYESTENGEKIEYEFQFAWDRQAS
                     FLNAQSRAMSELRSLIKQYDEMIHKDWNLATEEQKTRVEKLKCEVDNLSKDDIGDDEL
                     KISVDYGDRNDS"
     gene            30636..31889
                     /locus_tag="PC471_001583"
     CDS             30636..31889
                     /locus_tag="PC471_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HDF2788715.1"
                     /translation="MVIEMIVRVNFNPDFKEANFTKKRYRAMKGSAGSGKSVNVAQDY
                     ILKLGDKKYQGANLLVVRKSEATHKYSTYAELTGAINRIYSKQADKYWKTTLNPLEIK
                     SKVTGNSIIFRGVNDAKQREKLKSINFSKGKLTWVWCEEATELMESDIDILDDRLRGI
                     LTNPNLYYQMTFTFNPVSATHWIKRKYFDYKNDDIFTHHSTYLQNRFIDEAYYRRMQM
                     RKEQDPEGYKVYGLGEWGETGGAILKNYVIHEFPTEFEYFDNMRLSQDFGFNHANVVL
                     RIGFKDGELYICNEIYVHEMDTSEIIKIANNKGLEKNLFMYCDSAEPDRIKMWKSAGY
                     EAKGVKKGPGSVKAQIDYLKQLRIHVHPSCTNTIKEIQQWKWKQDERTGLYLDEPVEF
                     MDDAMAALRYSIDNKLKNNGISFLK"
     gene            31904..>32660
                     /locus_tag="PC471_001584"
     CDS             31904..>32660
                     /locus_tag="PC471_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HDF2788716.1"
                     /translation="MYISETDLIKVQLKKESTFNLVKVIEHYILKHRPEKYKQGEEYY
                     YGNADINNKRRYYLLDGAKVDDFTKVNNKAINNYHKLLVDQKVGYSVGNPIVFNADDD
                     NLTKLLNDLLGEEFDDTITELYLNASNKGIEWLHPYINRKGEFKYVIIPAEEAIPIWD
                     SKRQRELVAFIRFYYIEDIDGNKIKRVEYYTENDVTYFIERGNSFIQEFLYDEYGKMT
                     DIQEGHFRINNKEQGWGKVPFIPFKNNEKCVSDL"
BASE COUNT        13244 a         3393 c         5828 g        10195 t
ORIGIN      
        1 atgtggcaaa tagttgtttt gctatttatt atttgtttaa tatttctaat ttataaaaaa
       61 aaaattataa attttaaaaa ttataaatga cattttaact agaattaatt gtaaaaaaca
      121 ctatataata ctcaattttc aacattctat acaattatac agaaattata cagcataata
      181 atcaccgaaa taacaaattt taaaaattaa gcaaaacttt ttgtcgaagt tcaacgtcta
      241 tatatatgta gtgtaaattt ttgggataaa atactacaaa aatataaatg taaaaatata
      301 gtaatattgg aggtcgaacg atgagaaata aaaagaaaaa aataaataga aagaagttgt
      361 atttgctttt aagtgctatt gtagtgtgtc tggcttttat agtagttggt gttcgcgtca
      421 gctctttaaa tatgaaggaa aatgaaatta gtagaaatgc aaaactgtca agttctacta
      481 ttacagatat agtatctaat acatcaatag aagttagtaa agggccaagt aaaagttctg
      541 gaaaaatagc atacataact atagatgatg gtccatctaa atttactgac cagatgataa
      601 aaactttaaa taaatacaat gttaaagcaa ctttttttat gatagatggg aatatgaagg
      661 aatatccaca gcaagttaaa aatataatta aaaatgggaa cacagcaggt tttcatagtg
      721 tatcacatga tattcacaag ctttatgtta ctagtacatc agcaaaagag gaatttgata
      781 caaatgatca aactttttat aagataactg gtaaacattc aaaagtaata agaatacctt
      841 atggaagcaa accatataca ccacaagcct cttatcaggc tttagttgat gctggatata
      901 aaatttggga ctgggactta gatacagaag actggagatc taattcttct caaattgtac
      961 aaaatgtaaa aaatcatatt aagaatagaa aaggtgaaga taaagaccaa cttgtagttt
     1021 taatgcatga gaaaaaacag agtgcagaag ctttagattc agttttaaaa ttcttatcag
     1081 atgaaggtta tgaatttgcg ccagtagatc aaaaccaaat accaaaaaat tattggttac
     1141 gtaatttaga gtagttaaaa tataagaata ttgattgata tttttagaaa ttgagaataa
     1201 aaatgagtga ggtgtaggta tattgaataa aaaactttat ataggaatgg tcggaatatt
     1261 gtctttgatg atggttggtt gtaataaaag tttagcaaaa gtaaatgatg tggaaatcac
     1321 aaaagaacag tacaaaaaaa caaaagcagt tttatctgct actaataatt atataaatgg
     1381 gcagtcttta gatgaattag aaaaaacttt ggataaaaaa ggtagaaata aattagaaaa
     1441 tgttataata tcgtttatgg tggataatga acttttatat caagaggcta aggataaagg
     1501 actaactcca agtaaaagtg aagtagactc aaaatatcaa gaactagaag ataagatgaa
     1561 tttgaataca agctataagg aaaaaatgga caaagcaggt gttgataaag attatttaaa
     1621 acaagaaata tctagagatt tagcaataga taaaaataaa aaggcctttg aagatagaat
     1681 aaacataagt gataatgata tggaagccta ctatacaagt cataaaaaag attttaatgt
     1741 agaagaagta agtgcttctc agatacttat atcgacttta gataaaaata aaaaagaagt
     1801 aagcaaagat aaaaaagaag ctttgaagaa gaaggcagat aatatattga ctaaaatcaa
     1861 aaatggagaa agttttgaaa gtttggctaa aaaatattcg gatgataaag caactggaaa
     1921 aaatggagga cagctaggat attttacaaa ggatgataaa aatgctgagt ttacaaagga
     1981 agtgttcaaa ttaaagaaaa atgaagtttc aaatgtgttt gaaactagtt atggatatca
     2041 tatagtcaaa gttactgata aaagagaaag acaaaaaagt tttaatgagt gtcagagttt
     2101 aataagagaa tctattttaa atgaaaagta tattgagcat ataaaaaaac tgaatgaaga
     2161 tgctaaaata gacagataga atttctaaca gctcaatcct agcctgtaat cggagggaga
     2221 ttttacagga atgtttaaga gtttaaagag aacgaaagtt gagaaataca ttatcgataa
     2281 taaagactct ttttatagaa tagcatatag ttatacgaag aatgaagaag atgccttaga
     2341 tgtggttcaa gaagccatgt ataaggcact atactcagtg gaaaatatta aagaagtaaa
     2401 ttatataaag acatggtttt ataaaatttt agttagaacg agtatagatt ttattagaaa
     2461 aaaccgtaaa tataataata tgacagatat tgaccttata gatgagactg gagagtatga
     2521 taaatatact gatttggatt taagaagagc tttggaagaa ttacctattg aatacaaatc
     2581 tattataata ttaagatttt ttgaagattt aaagattgaa gaagtagcaa taatattgga
     2641 tgaaaatgtg aatactgtta aaactaggtt atatactgct ttaaaaaaac taaaattaaa
     2701 aattgaagag tagggataaa aagaatgaca agcaaagaaa ggttaaataa gttaaaagat
     2761 gagtatcata aaattccaat acccaagaaa ttagatacta taataaatca tgaaaaaata
     2821 gaaaacatat atcgagagaa aaataaaaaa gtaaatagat taagatttaa agttgcaata
     2881 gcctttgcat gtatatttac agtattagta aatataagtc ctgtatttgc agacaatttt
     2941 tctaagatac ctgtaattgg agcaatagta gaagtaataa ctataaaaaa ttatagttta
     3001 aaaagtgaaa attatgaagc tgagattgat attcctaaaa tacgaggttt aaaagataaa
     3061 aacttagaac aaagattaaa cagtagtttt atggaagatg gaaaaaggct ttatcatcaa
     3121 ttccaagaga gaatggagaa gatacaatca tcaaagaata aaggatataa atctttaagt
     3181 ttgagttata gtgtaaaaaa taattctaaa aaatttctat caatagagat gacaaaaaat
     3241 gagatagagg cttcatcata tgtgagtaag gtacattata ctattgataa aaaaagacaa
     3301 atagttttga ctttgccaat gctttttaaa gatgataagt atataaaagt tataagtgat
     3361 aatataaaag aacaaatgag agaacaaatg aaaaaggata gtacaaaatc ctattttata
     3421 gatcaaaaaa aagacttgcc tgttgaagat tttaaaacaa ttaataagta tcaggatttt
     3481 tattttaata aaaatgaaga ccttgtaatt tgttttgatg aatatgaagt agcacctggt
     3541 tatatgggcg cagttgagtt tgtaatacca tataaagtaa taaaagattt gtaaattgaa
     3601 tagtagttta tatataaatc ttttaaggag gacaatatga ctacatataa aaaacttaaa
     3661 aaatctatat ttaaaattgc tatattttca atatgcttat tagcttcatt agtagataca
     3721 aaccctatat ttgcaggtag ttcctttaat gaaacttcaa atagtgatac gataaaggtg
     3781 ttaaatgcta agagtatcaa ctttaaacaa gacaattatg aagtgaatgt gagtattcct
     3841 aaaataaaag gtttaaaaga taaaacttta gagaataaat taaacaatga gtttttgcaa
     3901 gatgggaaaa aaatttacag tgaatttcaa aataaaatga aaaaggaaga taataagggt
     3961 catagatacc taaatgtaat ccataatgtc aaaaataatt ctaaggactt tttatcaata
     4021 gaaatgacaa aagaagagat agaagcttcc tcatctataa ctaagaagca ttacactgtt
     4081 gataaaaata gaggaatact attaaattta ccaatatttt ttaaagatag tagatatatt
     4141 agagctataa gtgataatat aaaagagcag atgagaaatc aaattaagaa agatgataca
     4201 aaatcttatt ttatagacca agataaagac tcgccagtga aagattttga atctataaat
     4261 aagtatcaag atttttattt taataaaaat ggaaatcttg tgattagttt tgatgaatat
     4321 gaagtagctc ctggttatat gggagcagta gaatttgtga taccaaataa agtaataaaa
     4381 agactaagat agatatatag aagattttta gatagactgg taaggagaat tatatgaata
     4441 tatatgaaca agcaataaga ttaatagaaa ataatgagga tttcgcattt gctacaataa
     4501 cgtctcattc aggttcaaca ccacgtgaaa ctggagcaat gatgattgtg aaaaatgatt
     4561 ctactatatt tggctcagta ggaggaggta gtgtagaagc agaatgtata aaacatgcaa
     4621 ttaatgttat aaaaaacaga gaatcaatgt tgtataaatt tactctaaac aaatctgatg
     4681 ttgcaaaact aggaatgata tgtggaggta ctggagaaat acaaatagat tttatagata
     4741 gtaaattaaa aagtaatata gaaaaattta ataagagact aaaggaaaat acaagtaaag
     4801 catatatatt tggtgcaggt catatttcaa gagatgttgc tgtgatacta tctcttttag
     4861 aatttagaac agtagtaata gatgatagag aagaatttgc taatcatgaa aggtttccag
     4921 aatcagaagt agtagtttta gattcttttg aaaacatacc agattttcca actgacgaaa
     4981 atagttatat aattatttta acaagaggtc atctatatga ttcaagtgct ttagaatggg
     5041 ctcttaaaag agaggctggt tatataggta tgattggaag tagaacaaaa attggattga
     5101 cttacgaaaa actcatgaaa aaaggtttta agaaggaaga attatcaaaa gtacatgcac
     5161 caataggcat aaaattgaat gctcaaacac cagctgaaat agctgtatgt atagctgctg
     5221 aacttataaa ttgtagagct aataaggaaa aataaactaa tgaacacagg gagtaaagat
     5281 attgatactt taaaggttaa ttttagaggg attgaaataa aagcagtttt agtatataaa
     5341 aatagaaaaa atatttctat aaaaatagat ccatttggca aaataactgt aatgagtcct
     5401 cctaaaatat ccaaaaaaat aataaaagat ataattattg aaaaaggaga ctggatactt
     5461 aaaaagtcag aaaaatgtaa aggaagagag gaagtatata aacaaagaat gtttataact
     5521 ggcgaaaaat ttctttattt aggtgaagag taccatttag ttataaaaga actgttgaga
     5581 gattctgtca aaaaaagtaa ctgtagaata actattaacg aatatcaaat agttgttcag
     5641 acaaatgata caagtacaga ttttattaaa aaatctttaa aatcttggta taagatggaa
     5701 agtgaaagaa tagtattaga gagaatagat tttcttaaat taaattgtga aataatgaag
     5761 caattagttc cagcgagtgt taaggtaaaa gaacaaaata agagatgggg aagttgtaca
     5821 gctcaaaaaa atatttatat aaactcaaaa atatctatgg caagattaga tgtaattgat
     5881 tatgtaataa tacatgagtt tagccattta gtacatatga atcactctaa aaaattctat
     5941 gatttagtaa aatccattat gcccgattat aaagataaag aaaattggct aaaaataaat
     6001 ggatataaaa taataatctg aaaacgatta ttccaaaata taggcgtaaa gtataaaaga
     6061 agtattattg tgaaaaaaat aaataatctg aaaattttaa aaaaagtcat tgacattgta
     6121 aatgataggg aatataatct atattaacaa caaaacagaa ttaaaatata aaattaaaaa
     6181 attaaaagca atgacagaga caacaatact aatagtgtta tccacagaga accaatgttg
     6241 ctgagaattg gtgttaacag tagtataatg atcactctgg agcttttgta ctgaatattt
     6301 ttagtaagta caaacgtatg tcagcgttac atgactaagg ttaaggtaat taattacccg
     6361 acctgaaaaa agaggggtag atgtattttt tatatctaca aataaggtgg taccgcggaa
     6421 tataactttc gtccttataa gtaatattta tattgcttat aaagatgaaa gtttttttat
     6481 tttaagcgat ataaataaat ttaatccata taaaattaat aaaaaaataa taaaaataag
     6541 gagggatttt tatggaaaat actgtatatg ctaaattaaa ccaaggaata gattcttttt
     6601 taagagtggc tatgactcta agaagaagag aggttgatat acaatcaata agtatgactg
     6661 tagataatat gaataattca ggtataaaat taatagtaaa tgaagaaaag acatccttag
     6721 attctgtctt aaaccatatg aaaaaactac atgatataag agagataact gttgaaagaa
     6781 tgagacatta attaaatata ttttataaag ctaaagataa atatgcttat tttaaaataa
     6841 aaaactaaag ataaatatta aataagaaaa atcttagatt aaaatataag tgtcattgaa
     6901 tttattagtt tatcacagtt aataaattta ttagtttgtt gtagctaata aaaactgaaa
     6961 attctaaaat tataattgta agattatata aatttagaat ttaaatctgt tgaataatat
     7021 taaaacaatg ataatgagta tgaatttcta ataaaaataa atatcataaa aataagaaaa
     7081 agtattggga ggaaatgaaa atggcaagaa tgtattatga aaaagatgtg gatttagaag
     7141 ttttaaaaaa taaaaaggtt gcagttttag gttatggaag tcaaggacat gcacatgcac
     7201 aaaatcttag agataacggt gtacatgtag tgataggtct ttatgacgga agtaaatcag
     7261 cacaaaaagc aaaagaagat ggatttgaag ttaagagtgt agcagaagct acaaaagaaa
     7321 gtgatttgac aatgatgtta atgcctgatg aaaaacaaaa gaaagtttat gaagagagtg
     7381 taaaggataa cttaaaagaa ggccagacat tagcttttgc tcatggattc aatatacatt
     7441 ataatcaagt acaaccacca gaatttgttg atgttgtaat ggtagctcct aaaggacctg
     7501 gtcacttagt tagaaatgta tttacaaagg gaagtggagt tcctgcacta tttgcagtat
     7561 atcaagacta cactaaaaaa gctacagaga cagttttagc atatgcaaaa gggataggag
     7621 caacaagagc aggtgtttta gaaactactt ttaaagaaga aacagaaaca gatttatttg
     7681 gagaacaatc agtactttgt ggtggaataa gtgaacttat aaaattggga tataaaacgc
     7741 ttgtagatgc aggttatcaa aaagaagtag catattttga atgtttacat gaaatgaaac
     7801 taatagtcga ccttatatat gaaggtggat ttgaaagaat gagatatagt ataagtgaca
     7861 cagcagaata tggagattat gtatctggta aaagggtaat aactgatgtt gcaaaacaag
     7921 gtatgcaaaa tgttttagag gatatacaaa atggaaaatt tgcaaaagcg tggattaaag
     7981 aaaatgaaga aggacgtgaa aacttcctaa aaactagaga agaagaatat aatacagaaa
     8041 ttgccgaagt tggtagaaat ctaagaagta tgatgtcatt cttaaaataa atatatatta
     8101 aatggattag tttgagggaa agagtggtgt atagtagtgc gtatgaatgg agcaaaggta
     8161 attttagaat gtttaaagaa agagggaatt gacacaatat ttggatatcc tggtggagcc
     8221 gtgatacctc tttatgatgc tctatacgat tattcagatg attttaaaca tataaggaca
     8281 tcccatgaac aaggtttagt tcatgctgca gatgggtacg caagaagtac taatacagta
     8341 ggagtttgtt ttacaacatc tggtccagga gcaacaaatg ccataactgg aatagcaaca
     8401 gcttttatgg attcttcacc tatggttgta atttcagggc aggtgccaac tagtttatta
     8461 ggaaaagatt cttttcaaga aattgacata actggagcaa cattatctat gacaaaacat
     8521 aattatttag ttagaaacac taaagaatta gttcctacaa taaaagaagc ttttagagtt
     8581 gcaaattcag gtagaaaagg acctgtactt gtagatgttc caaaagattt atttttagca
     8641 gaaatggatt ttagtggtga ggactatgat ttatgtcaaa tagatgatta tatggattat
     8701 aagagtgatt ttgatctaga tgatgaaact aatattaagc ttttaaatga agcaatagat
     8761 atcataaaag aatctaaaaa gcctataata tatgcaggtg gaggtgttaa atcttcagac
     8821 agtgaggaaa ttcttgaaaa atttgcaact aagatagata cacccgtact aaatacactt
     8881 atggggcttg gaaatataga tagaaagaat gaattatctc ttggaatggt tggaatgcat
     8941 ggaagtagag aaagtaattt agcactttct aattcagatt tagtaatagc gataggtgcg
     9001 agatttagtg atagagtgat aagtaaaagt tctgaatttg ctaaaaatgc aaaaataata
     9061 cacatagaca tagacccatc tgaaataagt aaaaatatag aatctaatgt atctttggtt
     9121 ggagatgtaa agctagtcct tagtttatta attgaaaggg ttgaaagtaa gaataatagc
     9181 aattggaaag aagagataaa gagatttaga aagagtgagg gtgtacaaac atatgaattt
     9241 catccacaaa atatacttaa aaaaataaat gaaaaatatg aaacattaaa gaaacccact
     9301 gtggtagtaa ctgatgtcgg tcaacatcaa atgtgggctg ctaagtactg gaattttaaa
     9361 ggtaataaaa gttttataac atcagcaggg ttaggaacta tgggatttgg tttaggtgca
     9421 gctattggaa ctaaagttgg taatgtagat aaaaatgtgg ttttagtcac tggtgatgga
     9481 agttttagaa tgaactgtaa tgaactagct acagttgcaa attataatgt acctatgctt
     9541 attttactac tcaataatag aacattagga atggtaaggc aatggcaaaa gttattttcc
     9601 aatcaaagat actctcaaac tgacattaat gaaaatgtgg attatgttaa actcgtaaat
     9661 gcatataaca ttgatggata taaggtatcc agtatggaag aattaggaaa ggcgctagat
     9721 atgatagatt ttaataaacc tgtatttttg caatgtgata tagataagga ttatgatgtt
     9781 tatcctattg tagcacctaa tgatgcattg gaaaatttga tatgtaacta aaacttatac
     9841 tgaatattga taaaattttt tattaaattt ttattagtat tcagtttttg ttgtttagag
     9901 atagattggc attttttatt tttgcaaata aatcaatgac tatataatta agaacttatt
     9961 aaattttaat tatacaattt agaatagtat tacaacttaa gtaaatatta agttttattc
    10021 attagattta ttattaatac cttatattag ttatggttgt tatatactct aaagccttga
    10081 aattacatgc gtatagctga ctatataaat ttgataattt tagcttttaa aatagataaa
    10141 ttagattttg ccacccattt gccaccgtat tatatttttg ggtggcaaac tctaatttgt
    10201 aattttttca aatatatcta cagtttcatt tttcatttta tcagttacat gtgaataggt
    10261 atccattgta gttgatagtt ggctatgacc caaacggttt tgtatgtctt taatgttagc
    10321 accattttct aataatagag tagcatgtgc atgtcttaaa gaatgaaaat ggaagtcatt
    10381 gtttaaagct actcgaattt gtcttactat agtgtctaaa gtgtgagtat tcacttgttg
    10441 accattttct ttggtacata cccagtcact atcaaagtaa aattctccat attttaattt
    10501 cattttcttt tgatataatt tatgttcttt taatgccttt attaaagtgt cacctgtaaa
    10561 tatagttctg caagagcttt ctgtttttgg ttgccctaat tcaaacattc catttggctt
    10621 tttaatcaga gtatgtctta ctgtgatagt tttattatta aggtctatat tatcccattt
    10681 tagtgcaata atttcacctc ttcgcatacc agtatgaaat ccaattagta aaactatacg
    10741 ttgaaatgaa tcttgaggaa atatatttag tatttgatta aattcttcta ctgtaatagt
    10801 tttaacttta ttagtttctg ttttagattt agtttttggt atgcttacat attgcatagg
    10861 gttttctcgt atgtgtttgt aaggatggac tgctgatttt aatgacctat gtaatatggc
    10921 ttttaatact tgtaatgtat tttgagagta atcctctttg tactttttat ttatgaagtt
    10981 ttgtagtatt gcaggagtta aagcttttac tttgtaagct cctagctttg gctttatatg
    11041 tttttctatg tttattcggt agctttcttg agtgttgtat ttacagttaa gtaagacata
    11101 ttctttgtac caaaagtcta agtagtctga taaactgata ttgctttctt caaatactat
    11161 gccagaattt tcatattcat ttagtgcttc tcttaaggct ttttcggctt cttttttagt
    11221 attgcctcca actctttcta ctttttttct ttttccttct actatgccta ggtcaaagta
    11281 gtaataccat ttgttacttc tttttcttac tccacctttc ataatagtat ccctcccttt
    11341 tagaatgtat gtttgtttgg tgtttatata aaagagcaga ttaactgctc tttatatact
    11401 tttgttatgt acaatattat cttaattttg tttatctggt ttttacttaa ttattttctt
    11461 aatattctta caatttttta gcttttatgt gtagtttatt ataataatat tgaaatatat
    11521 atttattaag taaaaaatct ataaaagtag aataaatgag tttaaaaaat atcctcatat
    11581 atgtagaaat ttggcaaatt agttaatact aaaaatctat tatttcctaa gtccaacatg
    11641 cttttctttt ttgatagaaa ttctaatctt tttagtaaga agttgatgct aacttgtaat
    11701 tcttcagcta tttcgtatac acttgtagcg tgtgaattaa taacatgtat tatttcttct
    11761 tctgttataa gaaattcaca tgcccatttt aatgctttgt tttcagtttt gtctatcaag
    11821 attttatttt tgtaactgtt ttttgaagat acatagttcc caacactggt aaaatgatgt
    11881 cctaattctt ctgctaagat ttctattagt ttagcattgt tttgttttaa tgaattaagt
    11941 aatgatataa tctttagtcc ttgtctgttt atatacaatc cttttatgtc atctgctatt
    12001 ttgtcagtgt agtaaatttc tatctcttca ttatttgcta agtctaaaag tgcgtctagt
    12061 ttgttcattg aaatccccct ataaaaagaa tgtatttttg atttttgtta tatataaaga
    12121 gcaggtgtga actgctctaa atattcattt tttatccaaa aaagattgga taattttagc
    12181 tgtagacaaa atatggatta tagtatatga ttataatata attacatata atatatgtaa
    12241 gcacttacaa atgtgtgtta gtatagttag cactaaattt gtatttgtgc ttaaagcgat
    12301 taaaggagtt ttggagcggt gggtagagtt ttactcacta taaccaagac tcttttgcat
    12361 attagagtat ttcatgtagt catatattct aataagcctt cttaaatttg ttttttcgct
    12421 tgtttttgca gactctaaaa tattaggatt ttcctcaaat attttcttaa gtagattttc
    12481 taactgttca gtgtcaattt ctttttcttc taaaaaagca ataagtcttg tagctttttc
    12541 aaatgattga tgttcatcat tccaaatacc ataaacactt ctatttttta tgtaagtatt
    12601 atttctattt tttttaatac tattgctaat aatattttca aagtcacttt ctagtgcaat
    12661 tgtcctataa tctggaaatt caagtgtgct attaaaaaga tgtttattta atggtaattt
    12721 attatattgc ttaattaaag atggataagc atattcaagt aattcatctg atgaaaattc
    12781 taagtcattc atgactatat cacgatacca ttcatcaaaa cttaatcctt ttaatccttt
    12841 aagaccaaaa tcacttgcct tgccaactgc taaaactaaa tcttcatctt ttattcttgt
    12901 atcatcaaca ctaccaaccc gaattttagc agtaggtttg ttagtaattg tgaaatcgta
    12961 aaattcttgt ttgaacattc ttaatattct aactggtaat tttgattttt tttcagataa
    13021 tgcctcatag agtaaattaa aatcctccag tttaatttta gtcatagtaa tcattttatt
    13081 atcaaaagca attgtatgac ttgatatttc tatatcatta aagttttttt catactcaat
    13141 gaatatgaaa cgttcctcga gttttttagc attttcatta gatagacaat taactatagc
    13201 attaagtatt ttttttatat ttacatctgt aacagagtaa ccaataaata tgataggaaa
    13261 ctcaacaaat atagtcatta gcttagctgc caagtaggca cttttgctgt caaagtctat
    13321 ataatcagct tcatttatta caatactatt tggagaagaa acacatccat gaattttata
    13381 aatttctgca ataccttgta tagatgaaaa aactaaattt tcttgaccta tatatgttgt
    13441 ataattatca acgatagttt ccaaaaaaca atcataattt gttgtaataa atcctgataa
    13501 acttttttta gagatattac taagtttttc aacttcatct tgatatttat ctactatttt
    13561 agaattattt ttaatataca tagcaatttc tgccttaaat ggagaaactc ctgatttaac
    13621 aatatctaaa tacttagagt ctaagcttcg tatagaagaa tctgaaaacc attttttatt
    13681 aaaatcattt tcaataagtt ctgcaattct aggatttata cccatttttg ggttctctga
    13741 ttttgcttta tttttataac ttgtatatat aaagtcatca ttactaattt tttctgcaaa
    13801 gacttttagt aaatcctccc aatttggaag attatagtat cttctagtta aaccagaacc
    13861 aacaaataaa aatggtgtag cattaaatcg gtttatgaca tctgatatta tagatttcat
    13921 ataatttatc acctactttt aatttatttt ttatacttat ttattaaaaa ttcaatataa
    13981 tcattgagtt gttcttgagc ttcctctggt aaatctttgt gaggattaac tctatgtgca
    14041 gctacagtgt ctatatcatt ttttacaata gacctaccta ataaataatc agtagtcaca
    14101 ttaaatattt cagcaaggct agataacata taagcatcag gaactgtctt tccttgttca
    14161 taataaccat aagcgctttt ggttaagttt aatttatttg caacatcttc ttgagtcata
    14221 tttttttcag ctctaagttc ttttagtctt ttttgaaata atgtattatt acaagaagtt
    14281 ttgctagaaa tattgcttgc tttattatca tacctaccta ttaaataatc aacagtagtg
    14341 ccaaaatatt cagctaaact agataacatt tctggggtag gtgttctttt accttgttca
    14401 taaaatccat atgcactagt agttatgtta agatagttgg caatttcttt ttgcattaca
    14461 cctttttctt tcctaagaaa tttcaatcta tctgataaaa tgctcatata atcactcctc
    14521 ttaataaagt tgcctttatt aaataataca acaaatagtt gtaaaatgaa ataaaaaaat
    14581 aaaaaaacta tttacaaaca acatatagtt gtgtattata taagtataaa caacaaaatg
    14641 ttggtaagga ggatatagct tgaacaatct aaaggaactt aggacagaaa ggaatttaaa
    14701 gcaaaaagat gttgcattaa aagtaggtat aactactagt tattatggaa tgattgaaag
    14761 tggaacaaga gtaccaagtt tagcaatagc aataaaatta tctaaatgtt ttggagtatc
    14821 aatagaaaaa atttttttaa atacaaatac aacaagatgt tgtttaaaag agaggagtta
    14881 aatatgaaag atttaaaaat agtaaaagtt aataataaac taacaactga cagtagagat
    14941 atagctctaa tggttgaaaa agaacacaag attttactaa gggatataag aaattatata
    15001 aaccaaatgg aagaagccaa taaaaacatg agtacagatt tgtacccatc tgattatttt
    15061 attgaaaata cttatttaga tgattataaa agagagaagc catgctatgc aataacaaag
    15121 ataggttgtg acttcatagc aaataaaatg acaggcataa aaggtacggc atttacagga
    15181 atatatacaa aaaaatttga agaaatggag caagtattaa aaaatgaaca acctaaacta
    15241 ccaactacat ataaagaagc attacaacat cttatagagc aagtagaagt aaatgagaaa
    15301 ttacaactag aaggaaaaat gaaagaccaa gtaataaaag aactaaaacc aaaggcagat
    15361 tatacagata tgatacttaa aaacaaaggt ctagtcacta taactcaaat agcaaaggat
    15421 tatggaatga gtggaaaaga aatgaacaaa atacttcatg aaagagggat tcaatataaa
    15481 caaagtggac aatggctttt atataaacaa catcaaggga agggatacac tcattcagaa
    15541 acaatagaca taactagaag taatggaatg cctgatgtaa aaatgacaac taagtggact
    15601 caaaaaggta gattgttttt atatgattta ttgaaaacaa ataacatatt accagatata
    15661 gaaaaagaat atagttatca aacttcaata ttaggttagt actttgaaaa ctaaatacag
    15721 aatattttgg aaaggagaga ttgaattgga aaataagaaa atagaagaaa tagagaaaag
    15781 aataaccatg cttgaaaatc aattgcaagc acagttattt agtcagataa cagctcaatc
    15841 aaatagtgat tttatagatt atatgtacca atgtatagtt ttgtatttac atgagatgag
    15901 taaaagtgaa aaaagaaaaa ttagtgaaat tactaaagaa agaatcagaa aataagaatg
    15961 ataattacaa tataaaacct attcttctag caggttttga agggtcagtt ttctttactg
    16021 aagttatcat aaagtttatc tgtgatgtgt gttgaagaat ttcagaataa actccattta
    16081 ctatgccgta gtagtgaatt agacaagggt tatgataacc tacttcttca acaatcatta
    16141 atacatcttt tccaaagtta gtcaatttta gtgcaatttc atgttcatca tcaagttctt
    16201 tttcaaattc ctctatagat ttaagaataa ttttatattg aaattcagca tagtaaaaat
    16261 actctgtgtt agaagtatca gagactgtct ttcttatact ttcttgtaat tctttagata
    16321 tattgtttga aaactgcata atacaccatc cttttgaaaa ttttggaatt tattccatgt
    16381 ttatattata ccatgtagaa ctgaggtgaa tacaatgtta ataggcgaca atataaagca
    16441 aatactaaga aaaagagata taaaacctta taaattggca aaagaattgg atatagatgt
    16501 aagtggttta tataaattac tgagaaataa aagttctaat ccaactatag atactctaat
    16561 aaaattagct gattatttag atattacatt agacgaattg gttggaagat aaataattga
    16621 aaagagggga gagcattttc acatggatat ttctgaaagc ataacaaaac aatttagtga
    16681 tagcttaaaa agcttaatag aaatagagat aagtaaacaa gagacagata gagttaagag
    16741 tcaaactatt gaacaaaagg taaaggtact ggagcctaaa gatatagttg ttttaataaa
    16801 aagaggttat ccaaattatc taataacagt agaagaagca aggggaattt taaagttaga
    16861 tacagctttt atgcgtaggt tagtaagcac aggtttgata aaatcactag ttagaggtga
    16921 tggtagaaaa atttcaagat atgaagttga tgattttatt gaaagaaatc aaggtaaaaa
    16981 tttggatgaa cttttaaaag cagtagagag gggggatgaa attgctgagt cttgatacta
    17041 ataataagaa tgtagtaact cttaaaaaag atggaaaagt tatagcagat atagtattta
    17101 aagatattaa aactggtaag aaaatatcgg ttggaatatt aaataaaaaa gtgttggtaa
    17161 aataaccaac acacaaaaaa ataaaaaata aaatataaca caagtaaagt ataacacaaa
    17221 aaggagagat ttaaaatggt tgaagtgaat gtaaatgtaa aagttaaagt agaagcacca
    17281 gaatttacaa atgcactatt agtagtggcg aatgctttag gaggtcttaa tctaggaaaa
    17341 gcaatgcaga tagaacctat aaatataaca gatatgaaag aagagaagaa agttgaagtt
    17401 aaaaaagctg aaaagattaa aaaggtagaa gtaaaagaag acgttaaaga agaaatagca
    17461 gaagcaaagg aagaaataga aaagaataat gaaaatacaa ctagtgaagt taaatacaca
    17521 aaagaagagg taagaactaa ggcagcacaa gtaagtaagg caggtaagaa ggataagctt
    17581 aaggagttat ttggtgaatt tggagctagt aagttaagtg aagtaaaaga agaagattat
    17641 tcagctttta tgaacaaatt agaaagttta tagggggtac ataaatgcca ttacaacatg
    17701 caagacttag tgcgagtgga gcccatagat ggcttcactg cactcctagt ataaaattag
    17761 aagaaaacta tccaccatca actagtatat atgcagaaga aggaacagtt gcacatgaat
    17821 tagcagaagt taaattaatg ctagagtatg aaaaaataag taaaaaggca tataatgcaa
    17881 gaattaaaaa gatacagaaa agtgagtatt ataactctga aatggaggac tatatacaat
    17941 cttatgttga gaatgtagtt gaattagtaa atgatagtaa agctatatgt gatgatgtaa
    18001 tagtgatgtt agaagaaaga ctcgatttta gtgagtgggt tccagaagga tttggaacag
    18061 gagacgttgt tgtaatatct gatggtatac ttcaagttat agacctaaag tatggaaaag
    18121 gtttagaagt ttcagctata gagaatcctc aacttagact atatggttta ggggcatata
    18181 atcagtttga aatgctatac gatattgatt taattaaaac aacaataatt caacctagac
    18241 ttgataacat atcaagtgaa gaaatagaag ttactaaatt acttacatgg gcagataatg
    18301 ttaagaagaa ggctcaaatg gcttttaatg gtgaaggaga gtttgtaagt ggcagtcatt
    18361 gtggattttg tagagctaaa aatgattgca gaaaaagagc tgaggataac cttaaactag
    18421 ctagaaaata tgattttgct gacacatttg ctcttaataa atatgagata gcagatattt
    18481 taggttttgc taagaatata caagattggt taaaagatgt tcaaagttat gcactagaac
    18541 aagctgaaaa gcatggtgtt aaatatccag gatataagct tgtagaaggc agaagtaata
    18601 gaaagtatgt agatgagcaa gaagttgcta aggttttatt aaattcagat tatgatgaag
    18661 aaaaaattta taagccaagg accttaaaag gaattagtga tatggagaaa gctataggta
    18721 aaaagagttt tgctaaacta ctaagtgatt taattataaa accagttgga aaagctactt
    18781 tagtagtaga aagtgataaa aggtcagaaa ttaatagtat agattcagcg aaaaaagatt
    18841 ttgaaatata aatgggggat attacatgcc attagatgca ggtataaaag ataaattaga
    18901 tgaaagcata agtataattt tagatttaaa ggatagaaaa aagagtatcg aaaaagattt
    18961 agattatgaa aaagataatt tactagatat gttaaagata ctaaatatta cagaatataa
    19021 ctcaactgaa gaaaatgcaa aagttatagt gatggatttt aaaagagaaa gtttaataaa
    19081 ggataaagtg atgtcaactt tcctcgaagt aaatgacaat catcttgata gagtgtatat
    19141 accagaacat ataaaagtta gtccagtatg ctttgtatca gtaagagcaa aggataatta
    19201 aaaatattta attcctagta gctataaaaa gatttaaaag gggatataat tttatgagta
    19261 atcaagaaat aactaaacta gttgaaaata atttgaatct tgtacatttt agtataaata
    19321 gattttttaa gtcttatgta gagcaacatc catacttata tgaagagttt tatcaagaag
    19381 gatgtattgg gttatataaa gcagcattaa attatgatag taacagaggc aagttttcta
    19441 cagtggctgt ttcttacata agaatagaaa tgtttaaagc tttgcgttat ggcgaaaaac
    19501 attatagaaa ctctatagaa ggtttagatg taagtgtatt tgaagatagt aaaagaccat
    19561 taaaagatat acatggattt tatgaaattc aatttaacta tgatgttgat tatataagaa
    19621 aaattgcaag aaagtcatat ttaaaagata ttgataaaat agtagattgg attttacaag
    19681 ataaaaagat atctgatata ggagagttat taaatgtttc ttcaacagtt atttgtggaa
    19741 gaattaaatt gtttagtaag caagttagag atttagataa atttggagaa ataacttcaa
    19801 atataaaatc aatataccaa atataaaaat attaggagga agaaaattat gagtaattca
    19861 gtacaatcaa caaaggtagt aacaggaaag gtaagattaa gttattgcaa tatctttaaa
    19921 agcagagcaa tggtagaagg tgcagagccc aagtattcag tttgtatttt aataccaaaa
    19981 tcagataagg tgactttagg aagaataaag aaggctattg atgcagctaa agaacaaggt
    20041 aaaacttcta aatggggtgg aaaattacca ggtaatttaa aaacacctct tcgtgatgga
    20101 gatgcagaaa gagctgatga agcagaggaa tatgtaggaa tgtatttttt aaatgcaaat
    20161 agtactcaaa agccaggaat agttgataaa gacttaaatg aaatattaga taatacagag
    20221 gtatatagtg gttgttatgg aagagttagt ataaactttt tcccatataa tagtgcagga
    20281 aataaaggaa taggttgtgg cttacaaaat gttcaaaagt tagcagatgg agaagtactt
    20341 ggaggagcta gagctagtgc agaagcagat ttctcagatg actttgaata tgaagatgaa
    20401 gaagaggact tcttaagtta atgaggacct tatcaattga tatagaaaca tatagtgatt
    20461 tagatataaa aaaagttgga gtctacagat atgtagactc tgctaatttt gagatactgt
    20521 tatttgccta tgcttttgat aatgaagagg taaaagttat tgatttagta aatgatgaag
    20581 agttaccaaa agaagtaata gaagctttaa atgataataa agttataaaa tcagcattta
    20641 atgctaattt tgaaagaaca tcaataagta aatttttaaa tattaattta aaaccaaatg
    20701 agtggtcatg tacaatgata aaggcgttaa cactaggact tccaggaagt ttagatagtg
    20761 tgtctaaggc tttaaagttt aatgaagata aacagaaaat gaaagaaggt aaagcattaa
    20821 tacaatattt ctgtaaacct tgtaaggcta caaaagttaa caagggaaga actagaaatt
    20881 taccaataca tgatatggaa aaatggaata aatttaaaga atattgtaaa caagatgttg
    20941 tagttgaaag agaaataaga aacaaactta gtaagtataa gactaccgaa agagaaatta
    21001 aattatggta tttagaccag agaattaatg atactggtat taaagtggat acagagttaa
    21061 tagagaatgc aatagaatgt gataaaagat atactgaaaa acttacaaaa gaagcaatta
    21121 aaataactgg tctaaataat ccaaatagtc cagctcaatt aaaaaaatgg ttaagtgata
    21181 aagttggctt tgagattaca agcctaacaa aagaaagtat tccagaaata ttaaaacaag
    21241 ttgatgatga aaatgtagtt agaattttag aacttagaaa attaatgtcc aaaacttcta
    21301 taaagaaata tgaggctatg aaattagcta aaggtaatga caatagagta agaggtctac
    21361 tacagtttta tggggctaat aggactggta gatgggcagg aagattagta caagtacaga
    21421 atttaccaca aaatcatata gaggatttag acctagctag aaacctatta aaagaaggag
    21481 attttgattt aatagagctt ttatatgata gtgtaccaga tgtcttaagt caattgataa
    21541 ggacagcttt tataccaagt gaaggtcata gatttatagt gtcagatttt agtgcaatag
    21601 aagctagagt tatagcttgg cttgcaggtg agaagtggag actggatgtg tttaattctc
    21661 atggaaaaat atatgaagct agtgccagtc agatgtttaa aattccaatc gaaaatatta
    21721 aaaaaggttc tgaacttaga caaaaaggaa agctagcaga attatcgaat ggatatggcg
    21781 gaagtgtagg ggccttaatt tctatggggg ctattaaaat ggggcttaaa gaagaagagc
    21841 ttcaacctat tgttactgca tggagaaatg ctaatccaaa tataactaag ttttggtggg
    21901 atgtagataa ggcagctaaa aaagctataa aagataggac tatagtagaa attcaacatg
    21961 ggattaaatt tatttataat ccaggcgttt tatttataga actaccaagt agtagaagat
    22021 tatcgtatct aaggcctaag atagagccac atactacatt tagtggggat aagataacat
    22081 atgaaggtat ggaacagaca agtaaacaat ggaaaagaat agatacttat ggacctaagt
    22141 tagttgagaa tattgttcaa gctacagcta gagattgctt aagagaggct atgtttaatg
    22201 tgacagatgc aggttatagc attgtaatgc atgtacatga tgagcttgta attgatgtag
    22261 ataaaaaagg aggttcttta gaagaagtta acagtattat gggaaaagaa atatcttggg
    22321 ctaaaggtct tcctcttaaa gcagatggat atgaatgtga ttattataag aaagactagg
    22381 tgattgattt atggatataa aggccagtga aattgaacac ataaatgtaa gacatgatgg
    22441 ccaactcatg ctggccatag gaaaaaataa attagaaaca cattggaaaa ataaaagtat
    22501 tttatggtcc gaacttgtaa atagattaag taaaacatta agaactcaag aaacatatac
    22561 agaatatagg aagatgtcaa agactgaaaa agatagagtt aaagatgttg gtggatttgt
    22621 aggtggaagc ttaaaaaatg gaagacgaaa agcagagaat attgcaaata gaagtattat
    22681 aactttagat atagattatg cgaataaaga tatatgggag gatataacat tattaaatga
    22741 ttatgcttgt cttatgtatt ctacgcactc acatactgag aataatccca gatatagact
    22801 tgtaatacct ttaactagac cagtactacc agaagaatat caagcaattt ccaggatgat
    22861 agcagatact ataggaatag atatgtttga tgatactaca tatcaacctc atagacttat
    22921 gtattttcca agtacttcaa ttgatggaga ctatatattt aaatttcaag atggagagtt
    22981 tttaaaccca aatgaaatac tagatttata tttagactgg acagatgtaa gctattggcc
    23041 agaaagttcg agggagaggc aaaagtttaa tacacaatta aaaaaacaac aagaccctat
    23101 tgaaaaggct ggaattatag gtgcattttg caggtcctat agtataaaag aaactataga
    23161 aactttctta aatgaagtat atattcctgg tattgatgaa accaggtata catattcaga
    23221 aggtagtaca agtggtggag tagttattta tgatgacaag ttttcatata gtcatcatgg
    23281 tacagaccca gcaagcggaa ttttatgcaa tgcttttgat ttagttagga tacataaatt
    23341 tggtgaactt gatgaagatg ctaaacccga aacgcctgta aatagattac cttcatttac
    23401 tcgaatgagc gaatttgcaa gcagtgacac taaagtacga aagactatag gaagagaaaa
    23461 ccttgataaa gctaaggatg attttggtga tatagatttt gaagatgatg aatggttaac
    23521 taggttagat tatgacaata agggaagtta taagaaaaca acaaacaata tcttaatgtt
    23581 tatagaaaat gacccatatt tgaaaggaaa aatagcttat aatgaatttt caaatagagc
    23641 tgtcgtttta ggtaagttac cttggagaaa agatgataaa ttaaatgatt ggaatgatag
    23701 tgatgattct gggcttagac atcatataga aacaatttac aatatctcat caccatcaaa
    23761 agtaaatgat gctctaataa ttgcttttga aaataatact tttcatccta taaaagatta
    23821 tttaaattct ttaaagtggg atggcattaa gagagtggat acacttttaa ttgactattt
    23881 aggtgcagaa gataaccact atacaaggac tataataaga aaagttttag tagcagcagt
    23941 agcaagagta tttaatccag gaataaagtt tgataatatg atggttttat ctggtcctca
    24001 aggaatggga aaaagtactt ttattaaaaa acttggtgga gactggtatt ctgatagttt
    24061 aactactgta caaggtaaag aagcgtatga acaattacaa ggagtatggt tgctggaaat
    24121 gggtgaaatg atggctacta aaaaagcaga tattgaggca gttaagcatt ttctaagcaa
    24181 atcagaggat atatataggg tcgcatatgg gaagagaacc tcaagatttt tacgtcaatg
    24241 tgtagttata ggaacaacta atgataaaga atttttaaga gataagactg ggaatagaag
    24301 gttttggcca atagatgcag gagtaaagaa gactaacaag agtatattta atggccaact
    24361 tgataatgaa agaaatcaaa tttgggcaga agcagtagaa ttatataaag ccaatgaaca
    24421 gctatatcta tcagatgagg agaaaaaaga agctgaaaga caacaaagaa ctcactcaga
    24481 agaaaatgct aaatctggaa ttatcgaaga gtatctaaat aaacctatta ctaagaattg
    24541 gtatgattta agtatttcag aaaagagaga atatattcat ggttcagatt ttggtgattt
    24601 aaaagaaggg acaatattaa gagaaaaaac gtgtgttatg gagatatggg ttgagctatt
    24661 taatggagaa cctaaacaac tcacgcctat cttatcaaga gaaattaatg atatattaaa
    24721 aggattagat gattggatat catataatgg aaatttaagg tttgggaaaa tatatggtag
    24781 gcagagagct tatgtacgta aaagttagtg tcaacagaat taaaaataga gtaaatttga
    24841 aaattttaaa agtgtcaaca atgtcaacaa aaaaaatggc tttgttgacg cttatgttga
    24901 cacctaagat gcagtaatta caatgtttga ggtattaatg tcaacaatgt caacaaaaat
    24961 taagtataaa gtagtataag taatattagg catatacgta tatacatatg tatgcctaat
    25021 atgtatatat acatatatat agaaaatctg ttgacattgt tgacacttaa attttaagga
    25081 agtgatattt tgttagaatc aaaaatagaa aaaagactta aaaaagagat tgagttgtta
    25141 ggtggaaagg ctatgaagtt tatttcacca ggtgaggcag gtgtgccaga taggattgtc
    25201 ttattaccag aaggacatgt tatatttgta gaacttaaag caccaggtaa aaaactaaga
    25261 aagcttcaac aatataaaat gcgagaatta agagaattag gatttaaagt taagtgtgta
    25321 agcacattaa aagagataga tgattttatc aaggaggtta agggatggaa tttaaaccac
    25381 atccatacca agaatacaca attagaaaaa ctatagataa taataatata ggtttactat
    25441 tagatatggg attaggtaaa acagtctgtg ctctaacagc tataagtgaa ttaatgtatg
    25501 attactttga tatatcaaaa gttttagtta tagcaccttt aagagttgca agagatacct
    25561 ggagtagtga agtaaaaaaa tgggagcatc taaagcatct aaaagtatct aaagttcttg
    25621 gtagtaaatt agatagagtg agagctttaa gtacagactc agatatttat ataataaata
    25681 gagaaatggt accctggata gtggattttt ataaaagaaa atggccattt gatatggttg
    25741 tgatagatga acttagttct tttaaatcaa ataaagcaca acgatttaaa agtttaaaga
    25801 aagtattgcc tttaactaaa agagtagttg ggcttactgg tacaccaaca ccaaatagtt
    25861 taatagactt atgggcacag atgtacttac ttgatagagg tgaaagacta ggtaaaacta
    25921 ttacaggata taaagaacga tactttgagc caggacaaaa aaattatcaa acaggagcta
    25981 tatataattg gcagcctaaa gatggagcag aaaatgcaat acataataaa ataaaagata
    26041 tttgtataag cttaaaagca gaagattatt tgaatatgcc taaaaaaata gataataaaa
    26101 ttgaaataca ccttgatagt aaaatactta aatattacaa agaattagaa aaagagaaga
    26161 tattagaatt agaaaaagat ataataacag cttcatcagc agctgtagca gcaaataaac
    26221 ttttacaatt agctaatgga gcaatttatg ataatgataa aaatgtaaag gaactccata
    26281 gagaaaaatt agaagcttta aaagaaatta tagatgtttc aaatggtaag cctattatag
    26341 ttttttataa ctataaacat gattataatc gtttaatgaa agagtttaag agtttgaaac
    26401 caagaacaat agaaaactca aaagatatat atgattggaa taatggaaga atacaattat
    26461 tactttgtca tccagcaagt acaggacatg gacttaattt acaatcaggt ggaagtataa
    26521 ttgtttggtt tggattaact tggagcttag aactgtatca acaagccaat gcaaggcttt
    26581 atagacaggg gcaaagggaa actgttatta tccatcatct aatctgtaaa ggtacaatag
    26641 atgaacaagt aatggaggct ctagagaata aagataaagg acaaagtgca ttacttgaag
    26701 cagtcaaagc aaaattaaaa gaatataggg agtgaaggta tgaaagtaaa aagaaaagat
    26761 ggaacttata agcccaggta aaatagtaag atgtgcttgg tgtggtagaa gattttataa
    26821 gttggataaa tctaaagtta agtattgcag tagaagttgt tcgaagagag caagaaggag
    26881 tgatttaagt ggataaagaa agtataaaag aaattgtaag agagttaaga aaagaagaaa
    26941 gagaagatag gaaaaaaggc gttttatata atactagatt attgatgaag cattataatg
    27001 attttaagag acatatagac tcagcggtaa gtgaagctaa agatgtagat tacttagagg
    27061 atgatttagg taagcttgat aatgaagagt tgtatatttt aagtataaag aaaagtaaag
    27121 caagaactat tattatgata gcacatatag attcagcaat gaaaactttg aaattaagac
    27181 aagagcgttt acattcttat gaaaagtata gagctttaga gctttactat tgtgatgaaa
    27241 aaacatatga agaaatagca gagatattaa attgtggagt agttacatca agacgttgga
    27301 ttaatgaaat gataaaggaa ctgggagtgt atttattcgg tatagatggc ttgaaattaa
    27361 ctgtttgata aatatatgat aaaaagatga tattttaatg atattttaac cctgttataa
    27421 tggtagtatg aaacaagtgt ataatatatt cccccttgaa aaaggctaag ttaccccaac
    27481 ttagtctttt ttattttgta aataaggaga atgataaatg agtaattcat gacttagagt
    27541 gatttctagg tcttttttat acaattttaa tccctcatat tgaagggtcg aaaataaggg
    27601 tgttcaaatt gaatattctt tattaataac taatttagga ggtagtagta tgttgaaaat
    27661 tttacaagag aaaaatgtaa ggatgatatg gtcaaaaaat ggagaagaag tttgatttaa
    27721 tgcaaatgac gtaggagagg aactaggcat agtaaatatt cgtgatacat taagaaatat
    27781 agatagagaa tataaaaaga aattaatgag tctactgtcg gagattccta cactagaaac
    27841 tttaaagata aattgcctaa ctttggtact acttttgtta cagaagaagc tgtgtacaat
    27901 atgtcattta gaagtaataa agcagaagca aaactattta cgaagtgggt tacaaaagca
    27961 cttaaacaaa ttagaataca tggttactat attgccacag aaaaagatca agaatggctg
    28021 gatataagaa cagaaggtaa aaaggtcaga aaagatttta cagatgaaat acaagagttt
    28081 gtttattaat acaagcactt gaaagagtta tatctatgaa atcacctagg ttgatagata
    28141 aaagtatgaa ttataaagag gtatataaaa gattaaagaa ttaatagaaa tgatttaaat
    28201 gactgtatta atggagggtc ttttttttat gtaatgaatt aaaaatgaaa tatatttgta
    28261 cattatagac aggaataagg taaaagtatt atttagactt attttgtggt tgtcgaatga
    28321 ttattgaagg atattgactg attatataga attatatcat tataaatatt tgtgaaagga
    28381 ataatggtat aatgagtaat tttaaaaaaa gatatattga tgtttataga atagatatta
    28441 taaataaaat taatgggagt gtctattcag atgaagaaat tttaaaagag gtaataaaga
    28501 ccttctttaa ggataaagaa tattgtaaag aaaacaaaaa tggaattaat attactaaag
    28561 ataaaaagtt ttggattaca gaagtaaaat gtagcgagga tgatttaata atcaaggtga
    28621 aacttgaata tactaaatac aatcagaata caaatattat aaatgctcat actaaagaag
    28681 tagagggtaa taaaaaaatg gatcaaggag atagcaataa gcagcaccta tttattaaat
    28741 ttatgaagga aaataatata gcagtagtat tatttgaaag agtttttgtg ggggttccca
    28801 tgattgaact aagtaaaaac ttacatctat attatgagga tatgattgaa aataataaat
    28861 ttgataataa tatgtcaaaa acatcaatta atattacaca aatagcaagt aaagatttta
    28921 tagaacaaat attagattta gatggtgttt caaaaataac tttaaatgtt gatagagaga
    28981 agtttggaac tgatgaagat aatttgtttt ctaatttaaa taattcaaga cgacataacg
    29041 atttgatata taagcccaca tttagaacta gatatttacc tagtgaagtt aaaagatgtt
    29101 gcgaaaaata tattaaagga gaggaattta gaaataaaaa aataaatagg ataataattg
    29161 agggagagaa aaacaagaga aaattaaagt tggatactga gggtataaag ttgtgtagag
    29221 aaatagaggt ttctcttgat atagataatc atatagatag tgaagatatt tttgataaga
    29281 tgaataattt gatagatgat ttaattgaga ataattcagt tttttttaat ttaatgtatc
    29341 aagaagttgc tatgagtgaa gcatagattg cgaaggaggt gttaaaatgt ataaaaaggt
    29401 attaaatatc ttactcaatt attacaaggc tataaattct aaagaagtag tagagttagt
    29461 tttaacacca gtattaatat gtattatttc atacatgttt tttaattcgt caattaataa
    29521 agaagttgta atttctttaa acaaagatgt actaactttg tcaggactat tagtagcttt
    29581 tggagtttgc attattacat tattgtttac tacatataac aaatcaatat cagaagcaaa
    29641 agatataaaa acagaaagaa aggtaaatgg atttaatata tcatactttc aattcataca
    29701 gttaaaagca tattatactg taataatgga gattttggtt gttgtaatat gttttatcaa
    29761 tacaataatg cttactcgat attattctaa tataattttc tatgttttaa tattttttac
    29821 agttcatata attttatctc taactacatt aataataagt atgtttcatt tatcctggaa
    29881 ggatagagga gactagattt gaaacaatcc tattaaatag gattgttttt ttattcccaa
    29941 aacaaacaaa taaagaggtg gtgatgtgca agatgtcaaa gaaaaggtaa aacaagatta
    30001 cttaaaagga atgaaacaaa aggaaatatc atcaaagtat gacattagtt taaacacttt
    30061 aaagtcatgg ataaaaagat acaactgggc tagtgaaaaa aagaagggtg cacctataaa
    30121 taaaagaggt gcaccctttt ctaataaaaa ttcagttggt catggtgctc caaaagagaa
    30181 taagaatgct gaaaagtttg gtttcttctc aaagtatcta cccaaagaaa ctagagaatt
    30241 gatacaagaa atatctataa aagataaatt tgatattctt tgggaacaga taacaattca
    30301 atatgcagca ataataagag cacaaaagat aatgtatgtt aaaggcaagg aagaaatggt
    30361 taaagaatta aagaaatatg aaagcacaga aaatggtgaa aagatagagt atgaatttca
    30421 atttgcatgg gataggcaag catcttttct taatgcacag agtagagcta tgagtgaact
    30481 tagaagttta attaaacagt atgatgaaat gattcataag gattggaatt tggctacaga
    30541 ggagcagaaa acaagagttg agaagttgaa atgtgaagtt gataacctaa gtaaagatga
    30601 tattggagat gatgagttga aaataagtgt agattatggt gatagaaatg atagttagag
    30661 taaattttaa tccagatttc aaggaagcta attttactaa aaaaagatac agagcaatga
    30721 aaggttcagc agggagtgga aaatctgtta atgtagcaca agactatata ctaaagttag
    30781 gagataagaa gtatcaagga gctaatctat tagtagttag aaagtcagaa gctacacata
    30841 agtattcaac gtatgcagag cttacaggag ctataaatcg tatttatagt aaacaagctg
    30901 ataagtattg gaaaactact ttaaatcctt tagaaattaa gagtaaagtt actggtaact
    30961 ctataatttt cagaggagtt aatgatgcaa aacaaagaga aaaattaaaa tcaattaact
    31021 tctcgaaagg aaaattaaca tgggtttggt gtgaagaagc tacagaactt atggaaagtg
    31081 acatagacat actagatgac cgtttaagag gtattttaac taatcctaac ctatactatc
    31141 aaatgacatt tacatttaat ccagtctcag ctactcattg gataaaaaga aagtattttg
    31201 actataaaaa tgatgatata tttactcatc atagtactta tctacaaaat agattcatag
    31261 atgaggctta ctacagaaga atgcaaatga gaaaagagca agacccagaa gggtacaaag
    31321 tctatggtct tggagaatgg ggagaaactg gtggagcaat acttaaaaat tatgttatac
    31381 atgaatttcc tacagaattt gagtattttg acaatatgag gttatcacaa gactttggat
    31441 ttaaccatgc aaatgtagta cttagaattg gctttaagga tggagaatta tatatatgta
    31501 acgaaatata tgtacatgaa atggatacct cagaaatcat aaagattgca aataataaag
    31561 gtttagaaaa gaatctattt atgtactgtg atagtgctga accagataga attaagatgt
    31621 ggaagagtgc aggatatgaa gctaaaggag ttaaaaaagg accaggaagt gttaaagctc
    31681 aaatagatta tttgaaacaa ttaagaatac atgtacatcc tagttgcact aataccataa
    31741 aagaaataca acaatggaaa tggaaacaag atgaaagaac tggattatat cttgatgaac
    31801 cagttgagtt tatggatgat gcaatggctg cgcttagata ttctatagat aataagctta
    31861 aaaataatgg aataagcttc ttaaagtaaa ggaggcgtta aatatttata taagtgaaac
    31921 agatttaata aaagttcagt taaaaaaaga gagcaccttt aacctagtaa aagttataga
    31981 gcattatatt ttaaaacata gaccagagaa atataaacaa ggtgaagaat actattatgg
    32041 caatgcagat ataaacaata agagaagata ttatctctta gatggagcta aggttgatga
    32101 ttttactaaa gttaataata aagcaattaa caactaccat aagcttttag ttgaccaaaa
    32161 ggtaggctat agtgtcggaa atcccatagt atttaatgca gatgatgata atctcactaa
    32221 gcttttaaat gacttactag gagaagaatt tgacgataca ataacagaac tctacctcaa
    32281 tgctagtaac aaaggtattg aatggttaca tccatatatt aatagaaaag gtgagtttaa
    32341 atatgtaata atacctgctg aagaagcaat tcctatttgg gatagtaaaa gacagaggga
    32401 attagttgca tttattaggt tttattatat tgaagatata gatggaaata aaataaaaag
    32461 agttgagtac tacacagaaa atgacgtaac ttactttatt gaaagaggta atagttttat
    32521 tcaagaattt ttatatgatg aatatggaaa aatgactgat atacaagaag gtcattttag
    32581 aataaataac aaagaacagg gatggggtaa agttccattt atacctttta aaaataatga
    32641 aaagtgcgta tcagatttaa
//
LOCUS       DAMJYR010000034        32169 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.034, whole genome shotgun sequence.
ACCESSION   DAMJYR010000034 DAMJYR010000000
VERSION     DAMJYR010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 32169)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32169)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32169
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.034"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     regulatory      107..197
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            606..923
                     /gene="flgB"
                     /locus_tag="PC471_001585"
     CDS             606..923
                     /gene="flgB"
                     /locus_tag="PC471_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HDF2788717.1"
                     /translation="MNTYDIIKMGLDATNLRSQTIANNIANINTEGYKRHYITFEETL
                     NDAMPKIEEKTDNSKGLRVDGNNVDIEEEKVNQAMATLQYNALISFANGKIAMTKSII
                     SGR"
     gene            931..1335
                     /gene="flgC"
                     /locus_tag="PC471_001586"
     CDS             931..1335
                     /gene="flgC"
                     /locus_tag="PC471_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HDF2788718.1"
                     /translation="MSVFSGMRISASGLTAESLKMDIISSNVANINATRGPNGEAYRR
                     KVVSFEENYDNKLGMLGVKVTSVEDDKSPLKEVYDPSHPDAKPNGYVEYPNVNILVEM
                     TDLITASRAYDSNVDTLNAQKSMISKALEIGK"
     gene            1345..1662
                     /gene="fliE"
                     /locus_tag="PC471_001587"
     CDS             1345..1662
                     /gene="fliE"
                     /locus_tag="PC471_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HDF2788719.1"
                     /translation="MPITQINNLMQGAKLDNLIGEAKPLTLNNKEEKKNFEDVIIDTI
                     DKVNFQQVTADDMKEKFIKGEDVSMHEVMLIGQEAQLSMQYLIEVRNKIHDAYQEMNK
                     VQI"
     gene            1687..3237
                     /gene="fliF"
                     /locus_tag="PC471_001588"
     CDS             1687..3237
                     /gene="fliF"
                     /locus_tag="PC471_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar M-ring protein FliF"
                     /protein_id="HDF2788720.1"
                     /translation="MITSFKNKIKEQVAKSRTKISSLKKGTKIALGIGVIAICLALVF
                     TAISASKNKYAVLFSELDAQDAADISAELESKKIDTKVKGSTIYVPRTEVDKLRLELA
                     PSLTGGSKGFALMDDSSSIGLTDEEFQIKKQRMIQGEIEKTIKSFPQVEDTRVHITFG
                     ESSVFEKEAVPGSAAVYLMLKPGTTLEKNQIKSVMALISGSVTNIPEKNVQVIDNKMN
                     LLSEGILEDENVDENNQPINSGISVDKNKASEQALNKQLEKSILEMLEPIFGKGKVKA
                     TVNADLSFDTAEKTEIKIDPDKVAIKETRSKNSSKSAANNVQGVDANMNNQGNNNGTN
                     TEDSLDESFEYETGKIETHTIVAPGELNRLTASVAIDGKIAKGVQADVEDIVNNAIGM
                     DGARGDTLSVVSMVFDPEGKKEAKQELEEIKKEGFKKNIIKAVIGVVAVIAVGAIGYL
                     IYKKRSEREDMDYEDEDYISKSDSVDRKDNPLLGDDPELTLEEAVKMYAEEKPDQVTE
                     IIKTWLNE"
     gene            3250..4320
                     /gene="fliG"
                     /locus_tag="PC471_001589"
     CDS             3250..4320
                     /gene="fliG"
                     /locus_tag="PC471_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HDF2788721.1"
                     /translation="MSTEITNVNAFDLSDINESDKNEKVEGEGLKKAAVLLMTLGPNV
                     SSDIIKSLPDKQVQKIGLEIANISSIASKERREILKEFVEINRAKDYVIEGGLEYARE
                     LLSNALGNVKANKLLEGISINTSTKPFKHIIKLDVKKILEILRNESAQTIAVLLAHME
                     PENGAEVLSYLDFDLQNEVAMRISSMGSISPEVIKVIESSLEDKLHSISGDDFNDYGG
                     VSTLVQILINSDRKTEKNILGTIEEQNPDLAAQIKSHIFVFEDIVKLDDISIQKVLKE
                     VTMKDLVYALKGVDDEISDAIYRNQSSRAADALKEEMEMVGVIKVTQVEASQQKIANI
                     VRKLEREGAITIVRNFGGDFVE"
     gene            4313..5044
                     /locus_tag="PC471_001590"
     CDS             4313..5044
                     /locus_tag="PC471_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HDF2788722.1"
                     /translation="MNNKIIKSKKALYKGVLDLTIIDKVRVGESNVNKENDCRFRDEV
                     IKEAIESAEIRKNEILEEAKMNAQNIEKQAYEKGYSQGQKNGYEDGYKESYDEYTQKA
                     KDEADFIKNQADLILLQANEKMTEYIKEKNKEIVRLAINMASSVLKKHFEDEESMSDL
                     LKQIIQDYEGSSSFIVRCNSFYKDNIEKEFELFKEETSLKSSVFVIADDSIDKGNAII
                     QKENGRIIVGIDCAMEKLKEELLGE"
     gene            5048..6367
                     /gene="fliI"
                     /locus_tag="PC471_001591"
     CDS             5048..6367
                     /gene="fliI"
                     /locus_tag="PC471_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein export ATPase FliI"
                     /protein_id="HDF2788723.1"
                     /translation="MYKTDISIDFDKINKKIENLELTISEGRVKKIIGLTVEVEGIKA
                     FVGELCVIYNQVNKPVNCEVVGFKDKEVILMALGELTLIAPGCKVISKGIPLSVMCSD
                     NLLGKVLDGLGNPIDNSDAVLGDRYNLNNEPPDPMKRKKIRNIMETGVRAIDAFTTCG
                     EGQRIGIFAGSGVGKSTTLGMIARNAKADVNVIALIGERGREVLDFIDKDLGEEGMKK
                     SVVVCATSDKAPLVRLKGALTATAIAEYFRDQGKKVILMMDSVTRFAMAQREVGVAIG
                     EPPAQKGYTPSVFAMLPKLMERTGTSDKGSITAFYTVLVDGDDFNEPIADTTRGILDG
                     HIVLSRDLANKNHYPSIDVLNSLSRLMNEIASKEDIKIASFARDMLAEYREAEDLINI
                     GAYASGTNKKIDEAIYYHEHIINFLKQGINEKSSFNETISSLRRIFE"
     gene            6399..6845
                     /gene="fliJ"
                     /locus_tag="PC471_001592"
     CDS             6399..6845
                     /gene="fliJ"
                     /locus_tag="PC471_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012815984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export protein FliJ"
                     /protein_id="HDF2788724.1"
                     /translation="MELDKNFKFRLQKVLDLKIKDEEEIKMEFAKIQQKKIDIETNLE
                     NLESNYSKYSISKNNDNVQNQKITINYLLALNNSIMDLSEELDKSTNELEKARKQLIS
                     KQIERKSLEKLKEKKYGQYYKEEQLKEQSTNDEFASMSYLRNRQVL"
     gene            6873..8078
                     /locus_tag="PC471_001593"
     CDS             6873..8078
                     /locus_tag="PC471_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HDF2788725.1"
                     /translation="MKIQADSYNIIKDIGNRKNSSKNKNIKNNKFEDNLYKTTDHKNV
                     KDRRETSKQNVSSDRPGNSDNECHLDEDKSNHLEDENTYEKDCSKNNEALQQLLNLLK
                     ENYKKDKDINTILNNIKKLSISDELKQELKLNKNILNKIANQNLELNGANTNNPNDIE
                     NLDIMKLRELLENNASEDIQVISKTTENDTKSIDLYSFDSNRMDTLNSNKQKDDSSDI
                     LEKLAGLNGNKSGNFGQVINKSVDMNKIKNDNVNLKQIESNVMDDSIKAIKHMKTNDI
                     QELTIKLRPKELGDMNIQLLKDGESMRAVVTVFNKDVFDSINKNIADLKQHLELTNVN
                     IKDVSVQMHSDNTNTSDTFDKAFEQQNRQNNQENMSNQNTNDKRNNVVIEDEIKENII
                     DDDRVDLLA"
     gene            8096..8704
                     /locus_tag="PC471_001594"
     CDS             8096..8704
                     /locus_tag="PC471_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlgD"
                     /protein_id="HDF2788726.1"
                     /translation="MYGIATALAGTQVNYRSNNLSQLERNINDIANKQVRSKGNQTVT
                     DNGTPIIGQGDDADKDLFMKILVAQMGNQDPMNPQDPTEYVSQLAQFATLEKMSAMND
                     KLDILLALSNSTLINSALSMATSLIGKEVEVSGEEDEDENIVGVVKSTYIKDGEVHLE
                     LEVDGSDKTVDVKYEKLLKIGEVEANSGEENDSSEQESPEEV"
     gene            8731..9714
                     /locus_tag="PC471_001595"
     CDS             8731..9714
                     /locus_tag="PC471_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein"
                     /protein_id="HDF2788727.1"
                     /translation="MIKAMYSGVSGMKANQTKLDVIGNNVANAGTTSFKKSSARITDS
                     FYQTVLYASAPTAALGGTNLGQVGVGSKISSINKDMTQGNVQPTNRPSDLMIDGDGYL
                     PVVRQGTVMYTRDGSFNLDTGFKAADGALKNIKGGRLVNGDGYMLQGMIYDGEYKADT
                     GEFVKKGDAKGKEPIVIPLQSISPKDGTVQNVLEYNISKDGTVEFLLSDGQRTQFVYD
                     IPDGAKVGDTPAGIDKLKGTIQKVQTYAFQNPAGLDAEGGNLFKPSANSGDPQIAGAT
                     GTIIQGAIEASNVDIAEEFTEMIIASRSFQANSKIISTSDEILQEIINLKR"
     gene            9727..9930
                     /locus_tag="PC471_001596"
     CDS             9727..9930
                     /locus_tag="PC471_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar FlbD family protein"
                     /protein_id="HDF2788728.1"
                     /translation="MIRVTDLNGRKYILNSDLIVRIDSVPESRILLTTGEKNLVKESV
                     DEIVDKVIKYKRKINVGYIRSEK"
     gene            9930..10748
                     /locus_tag="PC471_001597"
     CDS             9930..10748
                     /locus_tag="PC471_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HDF2788729.1"
                     /translation="MKKTDSLTFIGLILSTVLVLFGAVKGSSSGLKSFFDFASILITV
                     FGSFGALMITFTIDDIKLIKDALQYSFKTMNISKLDLLNQFKTLSKKARREGLLSIES
                     DVMKIQDPFMKKGLELCIDGLEINEIRSILELEIDSVEETNNRYSKLFKTWGTFAPAF
                     GMLGTLIGLIQMLSDLTSPDLIASGMGKALITTFYGSLLANIALNPIAYNIDEKTEKE
                     IYVKEMMLEGIISIQSGESSIVVEERLATYLSNNEKLEIMKSNKNTERAMSNGA"
     gene            10738..11433
                     /locus_tag="PC471_001598"
     CDS             10738..11433
                     /locus_tag="PC471_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HDF2788730.1"
                     /translation="MARKKNKKKDEINPDAWLATYADTITLILTFFVLLYATSVVDME
                     KFKNIADALKGQFSGTSMFSTGSSTSEKSPIESVLVDKNDKSNLEQDLKDKVNNSNLQ
                     NSVTVKDDSRGILLELDDSILFDSGISELKEDSKATLNKVYDMIKMMKNKIVIEGHTD
                     NVPVKSSTHESNWDLSSSRAVSVVRYFVEEKGMDPKLFSATGCGEYQPLVSNETAKGR
                     AENRRVNILITID"
     gene            11445..11888
                     /locus_tag="PC471_001599"
     CDS             11445..11888
                     /locus_tag="PC471_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated FliL family
                     protein"
                     /protein_id="HDF2788731.1"
                     /translation="MFKNKKIIIIILILIIFSAGAGFFILGEGGNKQIDKIAGLFIPT
                     GKGKDEVKTENLDLEEMVLKLADEGVRYLKVSITVSYDSSYKDIEKNTPLIRDNIINC
                     FMAKRADDFTADNLKNIKEEIKNTLNIQLGENVIQNIYFTNIVVQ"
     gene            11903..12277
                     /locus_tag="PC471_001600"
     CDS             11903..12277
                     /locus_tag="PC471_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliZ"
                     /protein_id="HDF2788732.1"
                     /translation="MILYLLKILICIPIILILIVLSLKISQGTLLKKYNDKYIKILDI
                     VNISKNNSIIILKIGEEGCIMASSSSSMSKIKDLTKEEILRIENDIKERNFKIQDFKM
                     ERIKKKYDFSRYIGKFTKKEDK"
     gene            12278..12943
                     /gene="fliP"
                     /locus_tag="PC471_001601"
     CDS             12278..12943
                     /gene="fliP"
                     /locus_tag="PC471_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425215.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HDF2788733.1"
                     /translation="MELLSKLPLADVPYTSSMQLFLFLTALMFLPFVMFMMTSFVRIV
                     ISLSFLKSALGAQQAIPSQILVGLAIVLTIFIMRPVLNEINEKALQPYMKEEVTMEEA
                     MKEAEGPIKEFLLKQTRQTDLDLFVEQAGLKEKKLTRENIPLSVVVPAFAISELKTAF
                     QIGFLIYIPFLIIDLVVASVLMSMGMFMLPPVMISLPFKLLLFVMVDGWNLIVKTLIL
                     GFG"
     gene            12956..13225
                     /gene="fliQ"
                     /locus_tag="PC471_001602"
     CDS             12956..13225
                     /gene="fliQ"
                     /locus_tag="PC471_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HDF2788734.1"
                     /translation="MSEFMIMSVLKNGMFTGAMIAAPVLIVSLVVGLLISVFQATTQI
                     QEQTLTFVPKLIAILLVVAIGGSWMLHTLISFTSEIFNMMSLITK"
     gene            13237..15051
                     /locus_tag="PC471_001603"
     CDS             13237..15051
                     /locus_tag="PC471_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused FliR family export protein/FlhB family
                     type III secretion system protein"
                     /protein_id="HDF2788735.1"
                     /translation="MIFVFIRIVAFFGTLRILFPAGTPALFKSLFAIIISILISSTMQ
                     IEYATNIDNIVLFTLYGINETITGIMLGYITNLCFYSIRMAGSMMDQQMGLSMINMFD
                     PNSMTQTTLINNLMNWTALMIFFSMDGHHVLIRGIRYSFELIPIGKSFIDNNIDYIIN
                     IFVQCFLTGFKIAIPIVLCLLMADFILGLISRSVPQLNVMIIGMPLKILVGIALFIIS
                     IPLIVNQISHLLSQIPKMYEGTFALAPMFFMGSTDKTEEATPKKKGEQRKKGNIAKSK
                     ELPIAMTLLAFTLLVPTLFSYVVNTLKSSLNYFLSLDFYMNINYSSIEKLVIAGLMDF
                     FKIFLPIAIPFLVLGIVANLFQVGILFTGETLKPNLSKLNPISGFKNMFSMRSLSTLI
                     KDTAIISILAYIGYTFFQDNYLDILKLGNIYLPTLMYTVKDLVYSILSKICVAMIVIA
                     VADYVYQRYSHKKQLRMTKQEVKDEYKNSEGDPEIKAKIKQKQRQISSQRTMQAVPSA
                     TVIVTNPTHLSIAVRYEKGKDQAPVVVAKGADYLAFKIREIAKGNDIPIIENKPIARL
                     LYKQVEIDQEIPEDMYQAFAEILVAVYKIKNRYKVPKR"
     gene            15060..17135
                     /gene="flhA"
                     /locus_tag="PC471_001604"
     CDS             15060..17135
                     /gene="flhA"
                     /locus_tag="PC471_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HDF2788736.1"
                     /translation="MNKFNLKENMDVIVAFGVVGIVLMMIVPLPTFILDILLAINISL
                     SVLILLMTLFTTNVLDLSIFPTVLLVTTLFRLGLNLSSTRLILTQGYAGEVIEAFGSF
                     VVGGNYVVGIIIFLIILIVQFVVITNGSGRVSEVTARFTLDAMPGKQMSIDADLNAGV
                     IDDKTARKRRKELQQEADFYGSMDGANKFVKGDAIAGLIVTAINIIGGIVIGAVMLNM
                     TLMGAAQTYVRLTIGDGLVSQIPALLISTSAGIIVTRASTDENFGGLVGKQLTAIPKA
                     VMMTGCVLIVLGMMPGLPKPAFFSLGIGAIAAGYFLGREKKDTLEDLEFEKPDMVNPD
                     KNEVEDVTNLINVESIEVEIGYGLISLADESAGGDLLQRIASIRRQCAIDMGIVVQPI
                     RIRDNLQLNPNQYNIKIKGNVVGGYEIMPNMVLCMDPMNQGFSIFGIKTIEPTFGIEA
                     LWIESDKVEDAELKGYTVVDSSTILITHLLDIIKSNAHELLGRQEVKTIIDAARENYS
                     AVVDELIPDLMTLGEIQKVLKNLLREKVNIKDRVTILETLADQSRNTKDIELLTEYVR
                     IALARSICNNLVDEDKAIVVATLSPETEELVSNNLQRSVNGTYPAVDPENTNKIFESI
                     QEVMNNVYFNHNIPVIVVSPKIRAPFRKLVEIVYPNLTVLSLNEIPNDIKIKAEGVVS
                     IYDDEKVYS"
     gene            17110..18126
                     /gene="flhF"
                     /locus_tag="PC471_001605"
     CDS             17110..18126
                     /gene="flhF"
                     /locus_tag="PC471_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhF"
                     /protein_id="HDF2788737.1"
                     /translation="MMTKKYTANTIQDAMNLAKMELGDNITLIDKKEVRKSGIRGIFS
                     KKDIELTIGWEKKDNLEQKDLKREIEQLKSIINNMGFDNKNDNDIDKICKNLLNLDLN
                     QEIVEFIKADLQEMKFNGIDTSKNLVESLKKKIKIENQAINGKIALVGPPGVGKTTTI
                     AKLAAKLVFEENKKVGVITIDTYRIGAVEQLKIYTDIMNIPFKGVISPDEMELALDEM
                     KDCDVVLIDTTGRGYKNSMQILEIKNLIDKAETDNIHLVLNCTTRESDTKAIIDSYRN
                     VNFKSLIITKLDETITYGSIFNIMNYAQKPISYITTGQNVPDDIIKPNEDKIIRLLLG
                     VESI"
     gene            18123..19001
                     /locus_tag="PC471_001606"
     CDS             18123..19001
                     /locus_tag="PC471_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MinD/ParA family protein"
                     /protein_id="HDF2788738.1"
                     /translation="MIDQAQILRKMAMEKRGSDEIIVENENMPKIITIASGKGGVGKS
                     NLATNLSICLTKLDKKVLILDADIGMSNIDIIMGVNVKGTIIDVINGEKNIEDIISQT
                     KYGVNIISGGSALNHIEDFTEAQRNKFIHSIEQIHNVDFIIIDTGAGMSKSLLSFIYC
                     STEFFLITTPEPTSLTDAYSLLKAISNFGIKKSANIIINRVIDLEEAKSTYKRIKMVV
                     DKFLNLNLELYGYIIDDKKVGVCVKKQIPFILEYPTSIASKCIIAIAKRMVSQSREDI
                     VDNKSFLRKMFSIFKS"
     gene            19015..19716
                     /locus_tag="PC471_001607"
     CDS             19015..19716
                     /locus_tag="PC471_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliA/WhiG family RNA polymerase sigma factor"
                     /protein_id="HDF2788739.1"
                     /translation="MNREELIKENMPLVKSIARKFFIPGKGFEYQDLVNSGVIGLIDA
                     INKFDTEKGAKFSSYSYIKIKSAILDEIRNQSPISKHNLTKVNKYNRVVEKLQSKLLR
                     EPTSYEIAKELKVSEKELHDIESNIDMLNIVSLNYVIFEDTNETVQDVISDREEEAPE
                     NIIEEEEKLEILSKAISNLNEREKLILSLYYYEDLNLKEIGKVLGVSESRVSQLHRKS
                     IRNLRNKIKELKYSI"
     gene            19732..20154
                     /locus_tag="PC471_001608"
     CDS             19732..20154
                     /locus_tag="PC471_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein"
                     /protein_id="HDF2788740.1"
                     /translation="MIQIILIVVSILIIVTIVINLQKESKNKDNRYFDKIFEQEYEAN
                     DRLIIAEKRRFFLDKVLAEKRINSHIKVYENYNLIKANEERKENIFIESKKEVRQENE
                     LSLKVKHLISSGYDDVEICKMLDIGKGELLLIRSCYKI"
     gene            20139..20324
                     /locus_tag="PC471_001609"
     CDS             20139..20324
                     /locus_tag="PC471_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein"
                     /protein_id="HDF2788741.1"
                     /translation="MLQNIRILLCDKKVFLGIGIGILISTLCIMLFSNNDMSKAEIEK
                     KARALGMKYTSEMKVLE"
     gene            20365..21138
                     /gene="flgG"
                     /locus_tag="PC471_001610"
     CDS             20365..21138
                     /gene="flgG"
                     /locus_tag="PC471_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgG"
                     /protein_id="HDF2788742.1"
                     /translation="MIRGLYTAVSAMITNQQKQNVVVNNLANMDTNGYKSKQLLTKSF
                     DELKLEGYNNYANGTKQKQIIGDISPGISMDETITNFNQGPIKTTDNKMDVAIQGKGF
                     FLVSDAAGNQFYTRNGNFRTDNQGDLITSEGYYVLGTNTATGAVGHINVGNQKFEVAK
                     DNTISLDGNAMYKFNIVDFNNYNNLKKEGDNLYSGGGAMKVNNALTIQSAIESSNVDM
                     VSEINNMMTISREYEANQKIIQAMDSKLAKIASEIGSVR"
     gene            21159..21920
                     /gene="flgG"
                     /locus_tag="PC471_001611"
     CDS             21159..21920
                     /gene="flgG"
                     /locus_tag="PC471_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgG"
                     /protein_id="HDF2788743.1"
                     /translation="MYTMFYTSKTALSANQSKLDIISNNIANSDSTGYKKINMEYSDL
                     VDEVLNRPSYPTNGKDISTGTGAKATAPMRNYEQGALAPTDSKSNLAIDGEGFFRVIR
                     KDGTYAYTRNGGFNVDALGKIVDDNGNILDVQFDAGYNYNNTNIDSTNLTVSRNGELF
                     VDNKKIGKINLYQPIGTQNFISEGDSLFVATDAAQIKQVEKVDIVQGYRERSNVSLQE
                     EFVDLIATQRAFQMNSKGIKTADEMWQIANNLRAK"
     gene            21943..22824
                     /locus_tag="PC471_001612"
     CDS             21943..22824
                     /locus_tag="PC471_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor switch protein"
                     /protein_id="HDF2788744.1"
                     /translation="MNDMKEYDFGKPQSYSRENLTCLNFLLAEFCKKYKNYIIYELKC
                     NSSMVVDSIDQVNYQTFLDRVTSSCVVVQNAMEPVMKNFSFRIDRSVADMWIDITSGG
                     TGVVKDNDRELTELDKKVLLHLMDDLVRNMHLFEGFENIQVLDTHTHINLPQLCSPTA
                     PVCVVDLKVLNDNECIGSVSLCFPYNGLEALLESISVIEFFDNDVGDDSEEFTQKIYN
                     SVSNIELSVIAEIGKVSINVEDLLKLEVGDVIVTNKKINDYIDIFVENSKSYTATPGF
                     ISSKKGVKINDAVGKEV"
     gene            22827..23201
                     /locus_tag="PC471_001613"
     CDS             22827..23201
                     /locus_tag="PC471_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor switch protein"
                     /protein_id="HDF2788745.1"
                     /translation="MDNSNLISDEEAKMLLNDKVESEVDGDDELALDVSEVNRNNIQR
                     ILDLKLELSVVIGRTKMTLRDILNLHKGSLIELDTLADQDVEILIDNKVLAYGKVVVV
                     DLNFGVKITSIVSEEDMVKSLV"
     gene            23233..24636
                     /locus_tag="PC471_001614"
     CDS             23233..24636
                     /locus_tag="PC471_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HDF2788746.1"
                     /translation="MVLILNLNDIKKDILKLNKKDSCSLYNESINNINNNCISTGLKF
                     LKQARDLNPDDVDILNLTGLVNLLKCNFDDAVESFYKSSLCEKTALCEKYINMLTSEE
                     FLIFFEKYNQLIRLINKGMYKEAIQGFKLITDQYCDLIEPYELLALLYDKEEEYIKFD
                     ECLEVLKIIDKENYLLNQENNTLDMYNSANIQTKSNIKVDDYKNEFLHKSSSNHKIND
                     YNDVTKEKSSSKNQKSSDSNKKKSIKKNKYLYIAIILGILLVGQSIYNSYKMNNLTDK
                     ISKNNETKNEESIDSNLTKKENNIEENEDMSKASNINKSNKNINKEDDVKSKKNTVEN
                     EDDKDSDLFTEDELMSKANTLKSEKKTKSSIQLYKKVADIGENKGNTSEATYQVAILS
                     EKLKDYKTAEEYYKMYVENYSEKDAYFDESYYNLGMMYYNNGDLKNSKLTLKKLVNKV
                     PNSMYNNSKVKEILKEE"
     gene            24752..26689
                     /gene="htpG"
                     /locus_tag="PC471_001615"
     CDS             24752..26689
                     /gene="htpG"
                     /locus_tag="PC471_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HDF2788747.1"
                     /translation="MEFEKGSISIHTENIFPIIKKWLYSDKDIFIRELISNGCDAVSK
                     HKRLVSLGEISENKSSDYKITVSVNKGEGTLKFIDNGIGMTEEEIKKYINQVAFSGAE
                     DFFNKYKDKMEESNDIIGHFGLGFYSAFMVSKKVQIDTLSYTEGATPVRWISEGGTEY
                     EISESDARNDRGTTITLFIDDDSKEFLDEFTVRGIINKYCSFLPVEIYLEDVERLERE
                     AKEAEEKAKKQKEDGKEEIVDAKVIEPLNDTNPLWLKSPKDCTDEEYKEFYRKVFNVF
                     DEPLFWIHLNVDYPFNLKGILYFPKLKNEFELTEGKVKLYNNQVFVADNIKEVIPEFL
                     LLLKGVIDCPDLPLNVSRSFLQNDRDVSKISKHIIKKVADKLKSLCKNEREEYNKFWD
                     DIQIFIKYGCLKDESFYEKVKECILFKTIDDEYITLQDYLEKCKDKHENKVFYVSDKE
                     QQSQYIKLFKEYDLSAVVLNSSIDTHFISFMEYKENGVKFNRIDADLSDVLKDKNENK
                     DSEENKEEIAKIEGLFKEAVGERVKNYSVEGLKNEDTPAMVLVSEQSIRMAEMQSRFA
                     GMDLGMNFEEEKTLVINENSPIIKKLVSLKDDEEKKDKITLICNQIADLALLSNKELK
                     PDELDSFVQRSNKLMSLLIEL"
     gene            27077..28207
                     /locus_tag="PC471_001616"
     CDS             27077..28207
                     /locus_tag="PC471_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HDF2788748.1"
                     /translation="MNFNYNLPVNILFGSGRINDVGKEVSKYGKKVLIVTGKNSTKKT
                     GLLDKTIDLLKDSKIEYEVFDRVEQNPLITTIYSGVEIIKSTGCDCVLGLGGGSIMDA
                     AKSIAFSYKNPGDLNDYIFGIRQGEEALPIILVPTTSGTGSEGNCFAVLTNSETKDKK
                     SIKKNSMYAKASIIDPELMVTMPKHIIASVGFDALAHNMEAYLSNGRNPLADVQAIYG
                     IELISENLIKVYNDVNDLEAWEKITLASTIGGMTIGTAGTALPHGMEHPVSGLRDVMH
                     GKGLAALTPTIIESSWDSDIERFGNISKVLGGTCAEDCSDAIRNFLKKIDLDIKLSDL
                     GIEKSDIDWLTDNCMKISVANIKRHPKYFNKEQIKEMYHKSL"
     gene            28434..29456
                     /gene="splB"
                     /locus_tag="PC471_001617"
     CDS             28434..29456
                     /gene="splB"
                     /locus_tag="PC471_001617"
                     /EC_number="4.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore photoproduct lyase"
                     /protein_id="HDF2788749.1"
                     /translation="MFNPKRIIFEKGTLDTDVGKKIYNKFRDNKDVEIINISSNRIKE
                     HIPGENLYEQYRSGKQTMVVGIRKSLKFQTCKPSANYQLPLVSGCMGRCEYCYLNTQL
                     GDKPFIRVFVNVDEILEQTKKYIEDRKPQVTIFEGAATSDPVPVEPYTNSLKKTIEFF
                     GREEKGRFRFVTKYSDVDSLLDAEHNNHTEIRFSINTPRIINEHEHYTASANKRIESA
                     VKLARAGYQIGFIIAPVFLYEGWKEEYKNLIISIKEALPKDFDKKIIFEVISHRYTTK
                     AKNRILEIYPDTLLPMENEDRKFKYGQFGYGKYVYDKEQLDDMKEFFLKEINGVFKNS
                     LIKYII"
     gene            29638..30549
                     /locus_tag="PC471_001618"
     CDS             29638..30549
                     /locus_tag="PC471_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HDF2788750.1"
                     /translation="MHAWESIQITLDLIEANLSEEISINELANKANLSPFYYQRLFKR
                     LVNKTVMEYIKLRRLARASEYLAEHKNRILDVALNFGFASHETFTRSFKKAYGLTPEK
                     YRANPVKLNHFIKPELILNYAMVDEGVPLIADDIVIEVNRKTLSNPRTFVGIEIEVPI
                     CQLVGGETTGIAIVEELWMKLGSQRHNIPNQIPGGNEFAALYMGNAKEGNCMYMAGVE
                     VESGTSVEGYSTFELPAKEYLVCGFEAENFNELVNSAVFKADKFMERWMKKHNLTTTD
                     FAIEMYYPTTPEAAYLEHWIVPVPIEQ"
     gene            30564..30980
                     /locus_tag="PC471_001619"
     CDS             30564..30980
                     /locus_tag="PC471_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3788 domain-containing protein"
                     /protein_id="HDF2788751.1"
                     /translation="MNWQDAFFQDTKPTFEQINEFINNPLWDKLNKTLVSTYNVEPKL
                     EYSKCSMQRGWNVKYKKRGKSLCTLYPQEGYFKALVIVSESNRVEVDLFINTCCDYIK
                     KIYNEVNFFNGSKWLMIQVDSLLVLNDMLELIKFRA"
     gene            complement(31080..31472)
                     /locus_tag="PC471_001620"
     CDS             complement(31080..31472)
                     /locus_tag="PC471_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2788752.1"
                     /translation="MSINTEKNITKNVEELTCPIRYALDIVGGKWKLPIICMLAVENP
                     IRYSSIKRKLDGITNTMLAQSLKDLESTGIVHRKQYNEIPPKVEYTLTSKGKSIVPIL
                     QQFANWGATNMQEKNTCGLSCKECRKIK"
     gene            31613..32008
                     /locus_tag="PC471_001621"
     CDS             31613..32008
                     /locus_tag="PC471_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HDF2788753.1"
                     /translation="MKALEFLNKAGVYYLATVDENNQAHVRPLGFVMEYNGKLTFCTS
                     NQKDMFKQLIFNPKVELCCIDQNLNTLRILGEAVFVTSKETQAKALEIMPSLGQMYSV
                     GDGKFEIFSIDKAKVSCCSMSGKKIDVEL"
BASE COUNT        13204 a         3416 c         5884 g         9665 t
ORIGIN      
        1 tttataatct ttgtaattat tggtaattaa atttaaaaaa taaaatattt aattatacac
       61 aaaaaagata tttaaaaaat ttgttattta ttgtatactt aagttaaaac taaataggca
      121 aatctagaga aatctagtga cgcaaagcta tagggactaa ggtttatata cataaactat
      181 gtcagccagt tgccaaaaag agtcctaggt gtattgtata cctaagaaaa gtctataatg
      241 actgcctttt tggcagtcat tttgttatgg aaaaatcact ttatttcagt agaaaataca
      301 cataataaca tattttacca attataacat aaaataaaga aaaattatct caaaaagtta
      361 ctattttaca aaaaagagca acttttcgaa gaaatattta aatacatatt aaaatttttt
      421 tatattttca tgggacttca ttaaaaaaat attagttttc ttactaaatt gatacattat
      481 tgtattaatg taatggaaat gcgattaatt taaataaata aagaagtcat tttttgtaat
      541 atagcaactt catttttttt attaataaac aaaatagata aaatatattt tgaggagggg
      601 tgagtgtgaa tacatatgac attataaaaa tgggattaga tgcaactaat ctaagaagtc
      661 agacaatagc aaataatata gcaaatataa atactgaagg atataaaaga cattatataa
      721 cttttgaaga aacactaaat gatgctatgc ctaaaataga agaaaaaaca gacaattcta
      781 aaggtttaag agtagatggt aataatgtag acatagaaga agaaaaagta aatcaagcaa
      841 tggcaacatt acaatataat gctttgataa gttttgcaaa tggaaaaata gcaatgacca
      901 aatcaataat aagtgggagg taattagaag atgagtgtgt tcagtggaat gagaatatct
      961 gctagtggat taacagcaga aagtttgaaa atggatatca tatcatccaa tgtagctaat
     1021 ataaatgcta caagaggtcc aaatggagaa gcatatagga gaaaagttgt ttcatttgaa
     1081 gaaaactatg ataataaatt aggtatgcta ggagtaaaag ttacatctgt tgaagatgat
     1141 aaatcacctc ttaaagaagt atatgaccca tctcatccag atgcaaagcc aaatggatat
     1201 gtagaatatc caaatgtaaa tatattggta gaaatgacag atttaataac agcttcacga
     1261 gcatatgatt ctaacgtaga tacattgaat gctcagaagt ctatgatatc aaaagctttg
     1321 gaaataggaa agtagggata aattatgcca attacacaaa ttaacaattt aatgcaaggt
     1381 gcaaagttag ataatttgat tggagaagca aagccattaa ctcttaataa taaagaagaa
     1441 aaaaagaatt ttgaagatgt aataatagat actatagata aagttaattt tcaacaagta
     1501 acagcagatg atatgaaaga aaaatttata aaaggcgaag atgtttctat gcatgaagtt
     1561 atgttaatag gtcaagaagc acaactatct atgcaatatt taatagaagt tagaaataag
     1621 attcatgatg catatcaaga aatgaataag gtgcaaattt agccttttga acaggagtaa
     1681 taatcaatga taaccagttt taaaaataag attaaggagc aagttgctaa atctaggaca
     1741 aagataagtt ctttaaaaaa gggaactaaa atagcattag gtataggggt tattgctata
     1801 tgtttagcgc tagtatttac agcaatatct gcgagcaaaa ataagtatgc agtcttattt
     1861 tcagaactag atgctcaaga tgctgcagat atatcagcag agctagagtc taaaaagata
     1921 gatactaagg taaaaggaag tacgatttat gtacctagaa cagaagttga taagttaagg
     1981 ctggagttag cgccaagtct tacaggtggt tctaaaggat ttgcactaat ggatgatagt
     2041 tcatcaatag ggcttactga tgaagaattt cagattaaga aacaaagaat gatacaaggt
     2101 gaaatagaaa aaacaataaa gagttttcca caagtggaag acactagagt acatataaca
     2161 tttggagagt catcagtatt tgagaaggag gctgttccag ggagtgcagc agtttatttg
     2221 atgctaaaac cagggacgac tttagaaaaa aaccaaataa agtctgttat ggcacttata
     2281 tcaggaagcg ttacaaatat accagagaaa aatgttcagg ttattgataa caaaatgaat
     2341 ttattatctg aaggtatttt ggaggatgaa aatgttgatg aaaataatca accaataaat
     2401 agtggcatta gtgttgataa aaataaggca tctgaacaag cattgaataa acaactagaa
     2461 aagtctattt tagaaatgct agaaccgata tttggtaagg gaaaggtgaa agctacagta
     2521 aatgctgatt taagttttga tacagctgaa aaaactgaga taaaaattga ccctgataaa
     2581 gttgcaataa aggagacaag gtcgaaaaat tcatctaaat ctgctgcaaa taatgttcaa
     2641 ggtgtagatg ctaacatgaa taaccaagga aataacaatg gtactaatac agaagatagt
     2701 ttagatgaga gttttgagta tgaaacaggt aagattgaaa ctcacactat agttgcgcct
     2761 ggagaactta atagattgac agcttctgtt gcaatagatg gaaaaatagc taaaggtgtt
     2821 caagctgatg tagaggatat tgttaataat gctataggta tggatggagc gaggggagat
     2881 actcttagcg ttgtttcaat ggtatttgac ccagaaggta agaaagaggc taaacaagag
     2941 ctggaagaga ttaaaaaaga aggatttaaa aagaacataa taaaagcagt tattggagta
     3001 gtagcagtaa tagcagttgg agctattgga tacttaatat ataagaaaag aagtgaacga
     3061 gaagatatgg attatgaaga tgaagattat atttctaaat ctgattctgt tgataggaag
     3121 gataatcctc tattaggaga tgacccagaa ctgacattag aggaagctgt taaaatgtat
     3181 gcagaagaaa agccagacca agtaacagag ataataaaga cttggttaaa tgagtaaggg
     3241 ggatagaaag tgtccacgga aattacaaat gttaatgcgt ttgacttaag tgatataaat
     3301 gaaagtgata agaatgagaa ggtagaaggt gagggtttaa aaaaagctgc tgtattatta
     3361 atgacattag gcccaaatgt gtctagtgat ataataaaaa gcttgcctga taaacaagtt
     3421 caaaaaatag gtttggaaat tgcaaatatt tcttcaatag cttcaaaaga aagaagagaa
     3481 atcttaaaag agtttgtaga aataaatagg gcaaaagatt atgttataga aggtgggctt
     3541 gaatatgcaa gagagttatt aagtaatgct ttaggaaatg ttaaagcaaa caaattactt
     3601 gaaggtatat caataaatac aagtacaaag ccttttaaac atattataaa actagatgta
     3661 aagaagatat tagaaatact taggaatgaa agtgcacaaa ctatagccgt tttacttgca
     3721 catatggaac cagaaaatgg agcagaagtt ttatcatact tagattttga tttacaaaat
     3781 gaagtagcta tgagaataag ctcaatgggt tcaatatctc cagaagtaat aaaggtaatt
     3841 gaaagttcat tagaagataa attacattca ataagtggtg atgattttaa tgattatgga
     3901 ggggtgtcta ccttagttca aatattgatt aattctgata gaaaaacaga aaagaatatt
     3961 ttgggaacta ttgaagaaca aaacccggat ttagcagctc aaattaaatc tcatatattt
     4021 gtctttgaag atattgtcaa gttagatgat ataagcatac aaaaagtgtt aaaagaagtg
     4081 actatgaaag atttagtata tgccttaaaa ggtgtagatg atgagatttc agatgctata
     4141 tatagaaatc aatcttctag agcagcagat gcacttaaag aagaaatgga aatggttggt
     4201 gttataaagg ttacacaagt tgaggcttct caacaaaaga tagctaatat cgtaagaaaa
     4261 cttgaaagag aaggtgccat aactattgta aggaattttg gtggtgactt tgttgaataa
     4321 taagattatt aaaagtaaaa aagcattata taaaggagtg ctagatttaa ccataataga
     4381 taaagttaga gtgggagaat caaatgtaaa taaagaaaat gattgtagat ttagggatga
     4441 ggtaataaaa gaagctatag aatctgctga gataagaaag aatgagatat tagaagaagc
     4501 aaaaatgaat gcacaaaata tagaaaagca agcatatgaa aaagggtata gtcaaggtca
     4561 aaaaaatgga tatgaagatg gatataaaga gtcatatgat gaatataccc aaaaagctaa
     4621 agatgaagca gattttataa aaaatcaggc agacttaata ctattacaag ctaatgagaa
     4681 gatgactgag tacataaaag aaaaaaataa agagattgta agacttgcta ttaatatggc
     4741 tagttctgtt ctaaaaaagc atttcgaaga tgaagaatct atgagtgatt tgttaaagca
     4801 aattattcaa gattatgaag gaagtagttc atttatagta agatgtaatt ctttctataa
     4861 agataatata gagaaagagt ttgaattatt caaagaggaa acatcactaa aatcaagtgt
     4921 atttgttatt gcagatgata gtatagataa aggcaatgct attatccaaa aggaaaatgg
     4981 aagaataata gttggtattg attgtgctat ggaaaaacta aaagaagaat tgcttggtga
     5041 ataaagtatg tataaaactg atatatctat agattttgac aaaataaata agaaaataga
     5101 aaatttagaa ctcactatat cagaaggtag agtaaagaag ataataggac ttactgtaga
     5161 agttgaagga ataaaagctt ttgttgggga gttatgcgtt atatacaacc aagttaataa
     5221 accagtgaat tgtgaagtag taggttttaa agataaagaa gtcatattaa tggcgttggg
     5281 agaattaaca ttgatagcac ctggttgtaa agttatttct aaggggatac ctttaagtgt
     5341 tatgtgtagt gataatttac taggtaaagt acttgatggt cttggaaatc ctatagacaa
     5401 ctcagatgct gttttaggag atagatacaa cctgaacaat gaacctcctg acccaatgaa
     5461 gagaaaaaag atacgaaata taatggaaac aggtgtaaga gcaatagatg catttactac
     5521 ttgtggtgaa ggacagagaa tcggcatatt tgcaggaagt ggagtaggta agagtacgac
     5581 tctaggtatg attgctagga atgcaaaagc agatgtaaat gtaattgctc ttattggaga
     5641 aagaggaaga gaagtactag attttattga taaagattta ggtgaagagg gaatgaaaaa
     5701 atctgttgtt gtgtgtgcaa catcagataa agcccctcta gttaggttaa aaggtgctct
     5761 tactgctact gcaatagcag aatattttag agaccaaggc aaaaaagtaa tacttatgat
     5821 ggattcagta actagatttg ctatggcaca aagagaagtt ggtgtagcaa taggagagcc
     5881 accagctcaa aaaggatata caccatctgt atttgcaatg ttgccaaaac ttatggagag
     5941 aacagggaca tcagataaag gttctataac tgcattttat acagtattag ttgatggaga
     6001 tgactttaat gagccaatag cagatacaac aagagggata cttgatggtc atatagtttt
     6061 atctagagac ttggcaaata agaatcacta tccttctata gatgtattaa acagtttaag
     6121 taggcttatg aatgaaattg cttcaaaaga agacataaaa atagcgtctt ttgcaagaga
     6181 catgcttgct gagtatagag aagcagaaga tttgataaat ataggtgcat atgcttcagg
     6241 tacaaacaaa aaaatagatg aagcaatcta ttatcatgag catattatta attttttaaa
     6301 acaaggaata aatgaaaaga gttcttttaa tgaaactata agtagtttaa gaagaatctt
     6361 tgaataaaca atttaaaaaa atatttgaat aggttgtgat ggaattggat aaaaatttta
     6421 agtttagatt acagaaagtt ttagacttaa aaataaaaga tgaagaagag ataaaaatgg
     6481 aatttgctaa gattcaacaa aaaaaaatag atatagaaac taatttagaa aacttagaaa
     6541 gtaattacag caaatattct ataagtaaaa ataatgataa tgttcaaaat caaaaaataa
     6601 ctattaacta tttgctagct ttaaacaact ctataatgga tttatcagaa gaactggata
     6661 agtcgacaaa tgaacttgaa aaagctagaa aacagcttat aagcaaacag atagagagaa
     6721 agtcattaga aaaattaaaa gaaaaaaaat atggacagta ttataaagaa gaacagttaa
     6781 aagaacaaag cactaatgat gagtttgcaa gcatgagtta tttgagaaat agacaagttc
     6841 tataaaataa taggaagtga ggatattatt gtatgaagat acaagcagat tcttataata
     6901 ttattaaaga tattgggaat agaaaaaact catctaagaa taaaaatata aaaaataata
     6961 aatttgaaga taatctatat aagacaacag accataaaaa tgttaaagat agaagagaaa
     7021 caagtaagca aaatgttagt tcagatagac cgggaaatag tgataatgag tgtcatttag
     7081 atgaagataa aagtaatcac ttagaagatg aaaatacata tgaaaaagat tgtagtaaga
     7141 ataatgaagc tttacagcaa ttactgaatc ttttaaaaga gaattataaa aaagataaag
     7201 atataaacac tatattgaat aatataaaaa aactaagtat atcagatgaa ttaaaacaag
     7261 aacttaagct taataaaaat atattgaata aaattgcaaa tcaaaattta gagttaaatg
     7321 gagctaatac aaataaccct aatgatatag aaaatttaga cataatgaag ttaagagaac
     7381 ttttagaaaa taatgctagt gaagatattc aagtaattag taaaactaca gaaaatgata
     7441 ctaaaagcat agacttatac agttttgata gtaatagaat ggatactctt aactcaaata
     7501 aacagaaaga tgatagttct gatattttgg aaaaactagc agggttaaat ggaaataaga
     7561 gtggaaattt tggacaagta ataaataaaa gcgttgatat gaataaaatt aaaaatgata
     7621 atgttaattt aaaacagata gaatctaatg taatggatga ttctataaaa gctattaagc
     7681 atatgaaaac aaatgatata caagaattga caattaaatt aagaccaaaa gaattaggag
     7741 atatgaacat acaattatta aaagatggtg aaagtatgag agctgttgtg actgttttca
     7801 ataaagatgt ctttgactct ataaataaaa acatagcaga tttaaagcaa catcttgaac
     7861 ttacaaatgt aaatataaaa gatgtatcag tgcaaatgca tagcgataat acaaatacat
     7921 ctgatacctt tgataaagca tttgaacaac agaatagaca aaataaccaa gaaaacatga
     7981 gtaatcaaaa tacaaatgat aagcgtaaca atgtagtcat agaagatgag attaaagaaa
     8041 atattattga tgatgataga gtagatttac tagcttagta agggggaagt attgcatgta
     8101 cggaatagca acggctttgg caggaactca ggtaaactat aggtcaaata acttaagcca
     8161 acttgaaaga aatattaatg atattgccaa caaacaagtt agaagtaaag gaaatcagac
     8221 agtaacagac aatgggactc caattatagg acaaggtgat gatgctgata aagacttgtt
     8281 tatgaagatt cttgtagcac agatgggtaa tcaagaccca atgaatccac aagacccaac
     8341 ggagtatgta tcacaattag cacagtttgc aactttagaa aaaatgtcag ctatgaatga
     8401 taagctagat atacttttag ccttaagtaa ctctactcta ataaactctg ctttgagtat
     8461 ggcaacatca ttaattggta aagaagtaga agtatctggg gaagaagatg aagatgaaaa
     8521 cattgtagga gtagtaaagt ccacatacat aaaagatggg gaagtacact tagaattaga
     8581 agtagatgga agtgacaaaa ctgtagatgt taaatatgaa aaattactta aaataggaga
     8641 ggtagaggca aattcaggag aagaaaatga ctcttcagaa caagaatctc cagaagaagt
     8701 ataatattaa atgggaaaag aggtaaattt atgataaaag caatgtattc aggtgtaagt
     8761 ggaatgaaag caaatcaaac aaaactagat gtaatcggta acaatgtagc caacgctgga
     8821 actacttctt ttaagaaaag ttcagctaga ataacagatt cattttatca aactgtacta
     8881 tatgcaagtg ctccaacagc ggcattaggg ggaacaaact taggacaagt aggtgtaggt
     8941 agtaagatat caagtataaa caaagatatg acacaaggaa atgtacaacc aactaataga
     9001 ccatctgatt taatgataga tggagatgga tatcttccag tagtaagaca aggaacagtt
     9061 atgtatacga gagatggttc ttttaattta gatacaggat ttaaagcagc tgatggtgct
     9121 ttgaagaata ttaaaggtgg aagacttgta aatggtgatg gatatatgtt acaaggtatg
     9181 atttatgatg gggaatataa agctgatact ggagaatttg ttaaaaaggg tgatgctaaa
     9241 ggaaaagagc ctattgtaat accacttcaa agtataagtc ctaaggatgg aacagttcaa
     9301 aatgtactag agtataatat atcaaaggat ggaacagtag aatttttatt gagtgatggt
     9361 caaagaactc aatttgtcta tgatatacct gatggtgcta aggtaggaga tacacctgct
     9421 ggaatagata aattaaaagg tacaatacaa aaagtgcaaa catatgcatt ccaaaatcca
     9481 gcaggactag atgcagaagg aggaaacttg ttcaagccat cagcaaactc aggagaccca
     9541 caaatagcag gagctacagg cacaataata caaggagcta tagaagcttc caatgtagat
     9601 atagcagaag aatttacaga aatgataata gcaagtaggt ctttccaagc aaacagtaaa
     9661 ataatatcga catcagacga aatacttcaa gaaataataa atcttaagag atagtaggta
     9721 catattatga taagagtaac ggatttaaac ggaagaaaat acatattaaa ttcagatctt
     9781 atagtgagaa ttgactccgt gccagaaagc aggattctcc ttacaacagg agaaaaaaat
     9841 cttgtaaaag agtcagtaga tgagatagta gataaagtaa taaagtataa aagaaaaata
     9901 aatgtaggtt atattaggag tgaaaaataa tgaaaaaaac agattcacta acattcattg
     9961 gactaatatt atctacagta ttagttttat ttggagcagt aaaaggaagt agttcaggat
    10021 tgaaaagttt tttcgatttt gcatctatat taatcacagt atttgggtca tttggagctt
    10081 tgatgattac atttactatt gacgatataa aacttataaa agatgctcta caatactcat
    10141 tcaagacaat gaatatatcg aaattagact tattaaatca atttaaaaca ctttctaaaa
    10201 aagcaagaag agaaggactt ttatctatag aaagtgatgt aatgaaaata caagacccat
    10261 ttatgaaaaa aggattagag ttatgtattg atggtcttga aataaatgaa ataaggtcta
    10321 tactagagct cgaaatagat tctgttgaag aaaccaataa cagatattct aaactattta
    10381 aaacttgggg gactttcgca cctgcatttg gtatgttggg aactctaata ggtttaatac
    10441 aaatgttatc cgaccttaca tctccagatt taatagcatc tggtatggga aaagcattaa
    10501 ttacgacatt ttatgggtct ttacttgcca atatcgcttt gaacccaatt gcatataata
    10561 tagatgagaa gactgaaaaa gaaatttatg taaaggaaat gatgctggag gggataataa
    10621 gcatacaatc tggagagagc agcatagtag ttgaggaacg tttagctaca tacctttcaa
    10681 ataatgagaa gttagaaatt atgaaatcca ataaaaatac agaaagagca atgagtaatg
    10741 gcgcgtaaaa aaaataagaa aaaggatgag ataaatccag atgcatggtt ggctacttat
    10801 gcagatacga taacactgat tcttacattt tttgtgttat tgtatgctac atcagttgta
    10861 gatatggaaa aatttaaaaa tatagcagat gcgttaaaag gtcagttttc tgggacaagt
    10921 atgtttagta caggttcaag tacaagtgag aaatctccaa tagagagtgt tttagtggat
    10981 aaaaatgata agtcaaattt agaacaggat ttaaaagata aagttaataa ttctaactta
    11041 caaaatagtg taacagtcaa agatgatagt agagggattt tactagagct agatgattca
    11101 attttatttg actctgggat ttctgaatta aaagaagata gtaaagccac tcttaataaa
    11161 gtatacgata tgataaagat gatgaaaaat aagatagtta tagaaggcca tactgataat
    11221 gtacctgtaa aatcaagtac gcatgaatcc aattgggatt tatcttctag tagagcagta
    11281 agtgttgtaa gatactttgt agaagaaaag ggcatggacc ctaaactatt ttcggcaaca
    11341 ggatgtggag agtaccaacc tttggtatca aatgaaacag caaagggaag agctgagaat
    11401 agaagagtta atatacttat aactattgac taggaggaag aggtttgttc aaaaataaaa
    11461 aaattattat aattattttg atactgatta ttttctcagc aggagcaggt ttttttatat
    11521 taggagaagg tggaaataaa caaattgaca agattgcagg tttgtttatt ccaacaggga
    11581 aaggcaaaga tgaagtaaag accgaaaatc ttgacttaga agagatggta ttgaagctag
    11641 ctgatgaagg tgtaagatat ttaaaagtat caataactgt atcttatgat tcttcatata
    11701 aagatataga aaaaaatacg cctttaatta gggataatat tataaattgt tttatggcaa
    11761 aaagggcaga tgattttact gctgataatt taaagaacat aaaagaagaa attaaaaata
    11821 cattaaatat acaactaggt gaaaatgtaa ttcaaaatat atatttcaca aatatagtag
    11881 tgcaataaaa ggatggtaag acatgatact ttatttacta aaaatattaa tatgtatccc
    11941 aataatttta atattgatag tcttatcttt aaaaattagc caaggaactt tattaaaaaa
    12001 atataatgat aaatatataa aaatactaga tattgtaaat ataagtaaaa acaatagcat
    12061 aataatttta aaaataggtg aagaaggatg cattatggca tcttcttctt ctagcatgag
    12121 taaaataaaa gatttgacca aagaagaaat attaagaata gaaaacgaca ttaaagaaag
    12181 aaattttaaa attcaagatt ttaaaatgga aagaattaaa aagaaatatg atttcagtag
    12241 atatattgga aaatttacaa aaaaggaaga taagtaaatg gaactattat caaaattacc
    12301 tttggctgat gtaccatata caagttcaat gcaattgttt ttatttctaa ctgcattaat
    12361 gtttttacca tttgtaatgt tcatgatgac aagctttgtc agaatagtta taagtttatc
    12421 atttttgaaa tcagcattag gagcacagca agcaattcca agtcaaattt tggtagggct
    12481 tgcaatagta ttgacaatat ttataatgag accagtatta aatgagataa acgaaaaggc
    12541 attacaacca tatatgaaag aagaagttac tatggaagaa gctatgaagg aagcagaagg
    12601 acctattaaa gaatttttat taaagcaaac cagacagaca gatttagatt tatttgtaga
    12661 gcaagctggt ttaaaagaaa agaaattaac tcgtgaaaat ataccattat ctgttgtagt
    12721 tccagcattt gctataagtg aactaaaaac tgcttttcaa ataggttttt taatatatat
    12781 accatttttg ataatagacc ttgttgtggc aagtgttttg atgtctatgg gtatgtttat
    12841 gttacctcca gttatgatat cattaccatt taaattatta ttatttgtaa tggtggatgg
    12901 atggaacttg atagtaaaaa cattgatatt gggatttgga taggagaaaa atagtatgag
    12961 tgaatttatg ataatgagtg ttcttaaaaa tggcatgttt acaggagcta tgattgcagc
    13021 tccagtgcta attgtttctc ttgtagtggg gcttttaata agtgtatttc aagccacaac
    13081 tcaaatccaa gagcaaactt tgacttttgt accaaaacta atagcaattc ttttagtagt
    13141 agctattggt ggaagctgga tgttacatac tcttattagc tttacttcgg agatattcaa
    13201 tatgatgtct ctcataacta aataagtagg atatatatga tttttgtatt tattagaata
    13261 gtagcattct ttggaacatt aaggatacta tttccagctg gtacacctgc tttatttaag
    13321 tcgctctttg caatcattat atcaatcttg atttcaagta ctatgcaaat tgaatatgca
    13381 acaaatatag acaacattgt attgtttacc ttatatggta taaatgagac cataactggg
    13441 attatgcttg gatatataac caatctatgt ttttatagta tcagaatggc tggttctatg
    13501 atggaccaac agatgggatt atcaatgatt aatatgtttg acccaaattc aatgactcag
    13561 acaactttaa tcaataatct tatgaactgg acagcactta tgatattttt tagcatggat
    13621 ggtcatcatg ttttaattag aggcataagg tatagttttg agttaattcc aatagggaaa
    13681 tcattcatag ataataatat agattatata ataaacatat ttgtgcagtg ctttttaact
    13741 ggattcaaaa tagcaatacc tattgtactt tgtctgctta tggcagattt tatattaggg
    13801 cttatatcaa gaagtgtacc tcaactaaat gtaatgataa ttggtatgcc attaaaaata
    13861 ctggttggta ttgcattatt tatcatatca atacctttaa ttgtcaatca aataagtcat
    13921 ttattaagtc aaataccaaa gatgtatgag ggaacttttg cactagctcc tatgtttttt
    13981 atgggttcaa cagataaaac agaagaagct actccaaaga aaaagggaga acaaagaaaa
    14041 aaaggtaata tagcaaagag taaagaatta cccattgcaa tgactctact tgcattcact
    14101 ttattagtac ctacattatt ttcatatgta gtgaatacac ttaaatcttc tcttaattat
    14161 tttttgagtc tagattttta tatgaacata aattattcaa gtatagagaa attagttata
    14221 gcaggattaa tggatttttt taagatattt ttaccaatag ctattccatt cttagtttta
    14281 ggtatagtcg caaacctatt tcaagtagga atactgttta ctggtgaaac tttaaaaccc
    14341 aatttatcaa aattaaatcc aattagtgga ttcaaaaaca tgttttctat gagaagtttg
    14401 tcaaccttaa taaaagatac agcgattata tctattttgg cttacatagg atatacattc
    14461 ttccaagata attatttaga tatattaaag ttaggtaaca tatacctgcc aactttaatg
    14521 tacacagtta aagatttagt atattcaata ttaagtaaga tttgtgtagc tatgatagtt
    14581 atagcagttg cagattatgt ttatcaaaga tacagtcata aaaagcaact tagaatgaca
    14641 aaacaagaag ttaaagatga atataaaaac tcagaaggag acccagaaat aaaagctaag
    14701 ataaaacaaa aacaaagaca aatttcgtct caaagaacca tgcaagcagt accaagtgct
    14761 actgtaatag taaccaatcc cacccactta tctatagcag tgagatatga aaaagggaaa
    14821 gaccaagctc cagtagtggt ggcaaaaggt gcagattatt tagcttttaa aataagggaa
    14881 attgcaaagg gaaatgacat acctataata gaaaataaac ctattgctag gttgttatat
    14941 aagcaagtag aaatagacca agaaattcca gaggacatgt atcaagcttt tgcagagata
    15001 ttagtagcag tatataaaat caaaaataga tacaaagtcc ccaaaaggta ggataagtaa
    15061 tgaataagtt taatttaaag gaaaatatgg acgtaatagt ggcatttggt gttgtaggga
    15121 ttgttcttat gatgatagtg ccgttaccta catttatatt agatatttta ctggcaatta
    15181 acataagttt atctgtgcta attttactaa tgacactatt tacaacaaat gtacttgatt
    15241 tatctatatt tccaacagtg ttacttgtga caacactatt tagattggga ttaaatcttt
    15301 catcaactag attgatttta acacagggat atgcaggtga agttatagag gcatttggaa
    15361 gttttgttgt aggtggaaat tacgtagtag gaataattat attcttaatt atattaattg
    15421 ttcaatttgt tgttataacc aatggttctg gaagagtatc tgaagtaaca gctagattta
    15481 cgttagatgc catgccaggt aagcagatga gtatagatgc agatttaaat gcaggagtta
    15541 tagatgacaa aactgctaga aaaagaagaa aagagcttca gcaagaagca gatttttatg
    15601 gctctatgga tggagcaaat aagtttgtaa agggagatgc catagcaggt cttatagtaa
    15661 cagcaataaa cataataggt ggaattgtaa taggagctgt aatgctgaat atgactctta
    15721 tgggtgcagc acagacatat gtaagactta ctatagggga tggtcttgta agccagatac
    15781 ctgctctatt aatatctaca tcagcaggta taatcgtaac aagagcttct actgatgaaa
    15841 actttggtgg attagtaggt aaacagttaa cagcaatacc aaaagcagta atgatgactg
    15901 gctgtgttct aatcgtactt ggtatgatgc ctggattacc taaaccagca tttttttcac
    15961 taggtatagg tgctatagca gcaggttact ttttaggaag agaaaagaaa gatactttag
    16021 aagacttgga gtttgaaaaa cctgatatgg taaatccaga taaaaatgaa gtagaagatg
    16081 taacaaactt aataaatgta gaatcaatag aagtagaaat tggatatggt ttaatatctt
    16141 tagctgatga atcagcagga ggagatttac ttcagagaat agcatcaata agaagacaat
    16201 gtgcaataga catgggtata gtcgtacaac ctataagaat aagagataat ttacagttaa
    16261 atccaaacca atacaatata aaaataaaag ggaatgtagt tggtgggtat gagataatgc
    16321 ctaatatggt tttatgtatg gatcctatga atcaaggatt tagtatattt ggaataaaga
    16381 caattgagcc tacttttgga atagaggcac tttggataga aagtgacaag gtagaagatg
    16441 cagagctaaa gggttataca gttgtagatt catccacaat acttataact cacttactag
    16501 atataataaa atcaaatgca cacgaattac tgggaagaca agaagttaag actattatag
    16561 atgcagcaag agaaaattat agtgctgtag tagatgaatt gataccagat ttaatgacac
    16621 ttggtgaaat tcaaaaagta cttaaaaatc tgttaagaga aaaagtaaat ataaaggata
    16681 gagttacaat acttgagaca cttgcagacc agtctagaaa tacaaaagat atagagcttc
    16741 ttactgaata tgtaagaata gctttggcta gaagtatttg taataattta gttgatgaag
    16801 ataaggcaat tgtagtggct acattgtctc cagaaactga agaattggta tctaataatt
    16861 tacaaaggtc tgtaaatgga acttatccag cagttgaccc agaaaataca aataaaatat
    16921 ttgagagtat acaagaagta atgaacaatg tatatttcaa tcataatata ccagtaatag
    16981 tagtatcacc aaagataaga gctccattta gaaaattagt tgaaattgta tatcctaatt
    17041 tgactgtatt atcattaaat gaaataccta acgatattaa aatcaaagca gaaggtgtgg
    17101 tgagcatata tgatgacgaa aaagtataca gctaacacaa ttcaagatgc tatgaatcta
    17161 gccaaaatgg aattgggtga caatattaca ttaatagata aaaaagaggt aagaaaatct
    17221 ggaataagag gcattttttc aaagaaagat attgaattga ctattggatg ggaaaagaaa
    17281 gataatttag aacaaaaaga tttaaaacga gagatagaac aactaaagtc aattataaat
    17341 aatatggggt ttgataataa aaatgataat gatatagata aaatatgcaa gaatttacta
    17401 aacttagatt taaatcaaga aattgtagaa tttataaaag ctgatttaca agaaatgaag
    17461 ttcaatggaa ttgatacaag taaaaattta gtagaaagtc taaagaaaaa gattaaaata
    17521 gaaaatcaag caataaatgg taaaattgct ttagttggac caccaggagt aggtaaaacg
    17581 actacaatag caaagttggc tgccaaatta gtttttgagg aaaataaaaa agtaggagta
    17641 ataactatag atacatacag gattggtgct gtagaacagt taaagatata tactgatata
    17701 atgaatattc cttttaaagg ggtaataagc cctgatgaga tggaattggc acttgatgag
    17761 atgaaagatt gtgatgttgt attaatagat acaacaggta gaggctataa aaactctatg
    17821 caaatattag aaatcaaaaa tcttatagat aaagctgaaa ctgataatat acatttagtt
    17881 ttgaactgta caacaagaga aagtgataca aaagcaatta tagattcata tagaaatgtg
    17941 aattttaaaa gcttaataat tacaaagtta gatgaaacaa taacatatgg ctcaatattt
    18001 aacataatga attatgcaca aaaaccaata tcatatatta caacaggaca aaatgttcct
    18061 gatgatatta taaaacctaa tgaagataag attataagat tactattagg agttgaaagt
    18121 atatgataga ccaagctcaa attttgagaa aaatggcaat ggaaaaaaga ggttctgatg
    18181 agattatagt agagaatgag aatatgccta agataattac aatagcttct ggtaaaggcg
    18241 gcgttggaaa gagtaatctc gctacaaatt tgtctatatg cttaaccaaa cttgataaaa
    18301 aagttcttat attagatgca gatataggaa tgtctaatat agatataata atgggggtaa
    18361 atgtaaaagg gactataatt gatgtaatta atggcgaaaa aaatatagaa gatataattt
    18421 cacagactaa gtatggtgta aatataatat ctggaggttc tgcccttaat catatagaag
    18481 actttacaga agctcaaaga aataagttta tacatagcat agaacagatt cacaatgtgg
    18541 attttataat aattgataca ggagctggta tgagcaaaag cctattgtca tttatatact
    18601 gtagtactga atttttctta ataacaactc cagaaccgac atcattgact gacgcatata
    18661 gtttattaaa agctataagt aattttggca taaagaaaag tgcaaatata ataatcaata
    18721 gggtaattga tttagaagaa gcgaaatcta catacaaaag aataaaaatg gttgtggaca
    18781 aatttctaaa ccttaatctg gaattgtatg gatatataat agatgacaaa aaagtaggtg
    18841 tatgtgtaaa aaaacagata ccattcattt tagaatatcc tacaagtatt gcatcaaaat
    18901 gtattatagc tatagcaaag agaatggtaa gtcagtcaag agaagatatt gttgataata
    18961 aaagttttct aagaaagatg tttagtatat ttaaaagcta aggaggcgta gttaatgaat
    19021 agagaagaat taataaaaga gaatatgcct cttgtaaaga gtatagcaag aaaatttttt
    19081 ataccaggga aaggttttga atatcaagac ttagtaaata gtggagtcat tggattgatt
    19141 gatgcaataa ataaatttga tactgaaaaa ggagcaaagt tttctagtta ctcttatata
    19201 aagataaaat ctgctatatt agatgaaatt agaaatcaaa gtccaatttc taaacataat
    19261 ctaactaaag taaataagta taatagagtg gtagaaaagt tgcaatctaa attattaaga
    19321 gagccaactt catatgaaat tgcaaaagaa ttaaaggttt cagaaaaaga acttcatgat
    19381 atcgaaagta atatagatat gttaaatatt gtatcattaa actatgtaat ctttgaagat
    19441 acaaatgaaa ctgtacaaga tgtaataagt gatagagaag aggaagctcc agaaaatata
    19501 atagaagaag aagaaaagtt agagattcta tccaaagcaa ttagtaattt aaatgaaaga
    19561 gaaaaattaa tactttcact gtactattat gaagatttaa atctaaagga gatagggaaa
    19621 gtgctaggtg tttcagaatc aagagtttca caactccaca gaaaatcaat aagaaactta
    19681 agaaacaaga taaaagaact taaatattct atatagatgg tgattgataa gatgatacaa
    19741 attatattaa tagtagtgtc tattttaata atagtaacta tagttataaa tttacagaag
    19801 gaatcaaaaa ataaagataa cagatacttt gataaaatat ttgagcaaga atatgaagca
    19861 aatgatagat taataattgc agaaaaaaga agattctttt tagacaaagt acttgcagaa
    19921 aaaagaatta atagtcatat aaaagtttat gaaaattata acctaataaa ggctaatgaa
    19981 gaaagaaagg aaaacatatt tatagaatca aaaaaggaag taagacaaga aaatgagtta
    20041 tctcttaaag tgaaacatct tataagttca ggttatgatg atgtggaaat atgtaagatg
    20101 ctagatattg gaaaggggga actgttatta ataaggagtt gttacaaaat ataaggatat
    20161 tattatgtga taaaaaagta ttcttaggaa taggtattgg tatattaata tcaactttat
    20221 gtataatgct ttttagtaat aatgatatga gtaaggcaga gatagaaaaa aaggccagag
    20281 cgttaggcat gaaatataca agtgagatga aagttttaga atagatgata ctgtttagac
    20341 atttacaaaa aaggaggtca taatgtgata agaggattat atacagcagt ttctgctatg
    20401 ataactaatc aacaaaagca aaatgttgtt gtcaataacc tagctaatat ggatacaaat
    20461 ggatataaaa gtaaacaact tttgactaaa agttttgatg aattaaagtt ggagggttac
    20521 aataactacg caaatggaac taaacaaaag cagattatag gcgatataag tccaggtatt
    20581 tctatggatg aaactatcac aaattttaat caaggaccta taaaaactac tgataataaa
    20641 atggatgtag ctattcaagg taagggattt tttctagtta gtgatgcagc tggaaatcaa
    20701 ttttacacta gaaatgggaa cttcagaaca gacaatcaag gtgaccttat aacaagtgaa
    20761 gggtattatg tacttgggac gaatactgct actggtgctg taggtcatat aaatgtagga
    20821 aatcaaaaat ttgaagtggc aaaagataat acaatatcac tagacggaaa tgctatgtat
    20881 aaatttaata ttgtagattt taataactac aataacttaa aaaaagaagg agataactta
    20941 tattctggag gaggagctat gaaagtaaat aatgcactta caatacaaag tgcgatagag
    21001 tcctctaatg tagatatggt atctgaaatt aataatatga tgacaatatc aagagaatat
    21061 gaggcaaatc aaaagataat tcaggctatg gattctaaat tagcaaaaat agcaagtgag
    21121 attggttcag taagataggt aataggaggt gaatttttat gtacacaatg ttttatacta
    21181 gtaaaacagc tttatcagca aatcaaagta aactagatat aatatctaac aatatagcaa
    21241 attctgattc aacaggatat aaaaaaataa atatggaata ttctgattta gtggatgaag
    21301 tactaaatag accatcatac cctacaaatg gtaaagatat atcgactgga acaggagcta
    21361 aggcaacagc tcccatgaga aattatgaac aaggtgcatt ggctcctaca gatagtaaga
    21421 gtaatttggc gatagatgga gaaggattct ttagagtgat aagaaaagat ggtacatatg
    21481 catatacaag aaatggtgga tttaatgtag atgctttagg taagattgta gatgataatg
    21541 gaaatatatt agatgtccaa tttgatgcag gatataacta taataataca aatatagata
    21601 gtacaaacct gacagtttca agaaatggag agctattcgt agataataaa aaaataggaa
    21661 aaattaactt atatcaacct ataggaactc aaaattttat atctgaagga gatagtttat
    21721 ttgtcgcaac tgatgcagca cagattaaac aagttgagaa agtcgatata gttcaaggct
    21781 atagagagag gtcaaatgtt tctttacaag aagaatttgt tgacttaata gccactcaaa
    21841 gagcatttca aatgaactca aagggaataa agacagcaga tgaaatgtgg caaatagcga
    21901 ataatttaag agctaaataa ttatgtaagg agaggaatat tcatgaatga tatgaaagag
    21961 tacgattttg gcaaacctca gagttattca agagaaaatt taacttgttt aaatttttta
    22021 ttagctgaat tttgtaaaaa atataaaaac tatataattt atgaacttaa gtgtaattct
    22081 agtatggttg ttgatagtat agaccaagta aattatcaga cttttttaga tagagtaact
    22141 tcttcatgcg tagtagtaca aaatgcaatg gaacctgtta tgaaaaactt ttcttttaga
    22201 atagatagaa gtgtagctga tatgtggatt gatataactt ctggaggaac tggagttgta
    22261 aaggataatg atagagagct tacagaactt gataaaaaag tgcttttaca ccttatggat
    22321 gatttagtaa gaaatatgca cttatttgag ggttttgaaa atatacaagt attggataca
    22381 catacacata ttaatttgcc acaattatgt tctccaactg caccagtatg cgttgtagat
    22441 ttaaaagtgc taaatgataa tgagtgtatt ggaagtgttt ctctttgttt tccatacaat
    22501 ggtttagagg ctctgttaga aagtatatct gttatagaat tttttgataa tgatgtagga
    22561 gatgattcag aggaatttac ccaaaaaatc tataatagtg tatctaatat agaattatca
    22621 gttattgcag agataggaaa agtatcaatt aatgttgagg atttacttaa actagaagtt
    22681 ggagatgtaa tagtaacaaa taaaaaaata aatgattata tagatatatt tgtagaaaat
    22741 tcaaaatcat acacagcaac accaggattt atatcaagta aaaaaggtgt aaaaataaat
    22801 gatgctgttg gaaaagaggt gtagttatgg acaatagtaa tcttatttca gatgaagaag
    22861 caaagatgtt attaaatgat aaagttgaaa gtgaagttga tggagatgat gagttagcct
    22921 tagatgtctc tgaagttaat cgaaataata tacaaaggat acttgacctt aagttagaat
    22981 tatctgtagt tataggaaga actaagatga ctctaagaga tattttaaat ttacataaag
    23041 gttctcttat tgaactggat actttagcag accaagatgt ggaaatacta atagataata
    23101 aggttttggc ttatggaaaa gtagtggtgg tagatttaaa ttttggtgta aagataacaa
    23161 gtatagtaag tgaagaagac atggtaaaat ctcttgttta gagataacat caattaatac
    23221 ttaaggcatg aggtggtact tattttaaac ttaaatgata taaaaaaaga tatactaaaa
    23281 ctcaataaaa aagactcatg ttctctatat aatgaatcta ttaataatat aaataataat
    23341 tgtatatcaa ctggattaaa atttttaaaa caagcaagag atttaaaccc agatgatgtt
    23401 gatatattaa atctcacagg tcttgtaaac ttactaaaat gtaattttga tgatgctgta
    23461 gaaagtttct ataagagttc actttgtgaa aaaactgctc tgtgtgaaaa gtatataaac
    23521 atgcttacat ctgaggagtt tttaattttc tttgaaaagt acaatcaact tataaggtta
    23581 ataaataaag gaatgtataa agaagctata caagggttta aactcataac tgaccaatac
    23641 tgtgatttaa tagaacctta tgagttgctt gccttactgt atgacaagga agaagaatat
    23701 ataaaatttg atgaatgttt agaagtattg aagattattg ataaggaaaa ttatttatta
    23761 aatcaagaaa ataacacatt agatatgtat aatagtgcta atatacaaac taaaagtaat
    23821 ataaaagtag atgattataa gaatgagttc ttacataaaa gtagtagtaa ccataaaatt
    23881 aatgattata atgatgtaac taaagaaaag agttcaagca aaaaccagaa aagttcagat
    23941 tctaataaga aaaaaagtat taaaaaaaat aagtacctat atatagccat aatattagga
    24001 atattattag taggacaaag tatatataat tcttataaga tgaataattt aactgataaa
    24061 atatctaaaa ataatgaaac taaaaatgaa gagtcaatag attctaactt gactaagaaa
    24121 gaaaataata tagaagaaaa tgaagatatg agtaaggctt ctaatataaa taagagtaat
    24181 aaaaacatta ataaagagga tgatgtaaaa tctaaaaaaa atactgtaga aaatgaagat
    24241 gataaagatt ctgacttatt tacagaggat gaattgatgt ctaaggcgaa tactttaaaa
    24301 tcagaaaaga aaactaaatc ttctatacaa ctatataaga aagttgcaga tataggtgaa
    24361 aataaaggga atacatctga agcaacttat caagttgcta ttctcagtga aaagttaaaa
    24421 gactataaaa ctgctgaaga atattataaa atgtatgtag aaaattactc agaaaaagat
    24481 gcttattttg atgaatctta ttataattta ggtatgatgt actataacaa tggtgattta
    24541 aaaaattcta aattgacatt aaagaagttg gttaataagg taccaaatag tatgtataat
    24601 aattcaaaag taaaagaaat tttaaaagaa gaatagttaa attagataag aaaaataaat
    24661 tattttattt taaaataata tgattataat atattgacat accattttaa aaaatttaaa
    24721 atattaatta taaaatgagg agggataaaa aatggaattt gaaaaaggga gtatttcaat
    24781 tcatacagaa aatatttttc ctataattaa aaaatggtta tactctgata aagacatatt
    24841 tataagagag cttataagta atggatgtga tgcagtaagt aaacataaaa gacttgtatc
    24901 actaggagaa atatcagaaa ataagtcatc agattataaa attacagtat ctgtaaataa
    24961 aggagaaggt acacttaaat ttattgacaa tggaattggt atgactgaag aagagataaa
    25021 aaaatacata aatcaagttg ctttttcagg tgctgaagat ttctttaata aatataagga
    25081 taaaatggaa gagtcaaatg atataatagg tcattttgga ttaggatttt attctgcatt
    25141 tatggtgtct aaaaaggtac aaatagatac actttcatat acagaaggag cgactcctgt
    25201 aagatggata tctgaaggtg gtacagaata cgaaatatct gaatcagatg ctagaaatga
    25261 tagaggaact actataacac tatttattga tgatgatagt aaagaatttt tagatgaatt
    25321 tactgtaaga ggaataataa ataaatactg ttctttctta ccagtagaaa tatatttaga
    25381 agatgttgaa agattagaaa gagaagctaa agaagcagaa gaaaaagcta aaaaacaaaa
    25441 ggaagatgga aaagaagaga ttgtagatgc aaaagtaatt gagcctttaa acgacactaa
    25501 tcctctatgg ttaaaatctc caaaagactg tactgatgag gagtataaag agttttatag
    25561 aaaggtattc aatgtatttg atgaaccttt attttggata catttaaatg tagattatcc
    25621 tttcaattta aagggtatac tttacttccc taaattaaaa aatgagtttg aactgactga
    25681 aggaaaagta aaattataca acaatcaagt gtttgtggct gacaatataa aagaagttat
    25741 accagaattt ttacttttat taaaaggagt tatagactgc ccagacttac cattaaatgt
    25801 atcaagaagt ttcttacaaa atgacaggga tgtaagcaaa atttctaaac atataattaa
    25861 aaaagtagct gataagttaa aatctctttg taaaaatgaa agagaagaat acaataagtt
    25921 ttgggatgat atacaaatat ttattaaata tggttgctta aaagatgaaa gtttctatga
    25981 gaaggtaaaa gaatgtatat tattcaaaac tattgatgat gagtatataa cattacaaga
    26041 ttatttagaa aagtgtaagg acaaacatga aaataaggta ttttatgtaa gtgataaaga
    26101 acagcaatct caatacatta aactatttaa agaatacgac ttaagtgctg ttgttttaaa
    26161 ctcatcaata gatactcatt ttatttcatt tatggaatat aaagagaatg gagttaagtt
    26221 taatagaata gatgcagacc tttctgatgt attaaaagat aaaaatgaaa ataaagatag
    26281 tgaagaaaac aaagaagaga tagctaagat agaaggtctg tttaaagaag ctgttggaga
    26341 aagagtgaag aattattctg tagaaggatt gaaaaatgaa gacactccag ctatggtgtt
    26401 agtatcagaa caatctatta gaatggcaga aatgcagtca agatttgctg gaatggattt
    26461 aggaatgaat tttgaagaag aaaaaactct tgtaataaat gagaacagtc caataatcaa
    26521 aaaattggtt tctcttaaag atgatgagga aaagaaagat aagattacgc ttatttgtaa
    26581 ccagatagca gatttagctt tactttctaa taaagaatta aagccagatg agttagattc
    26641 atttgtacaa agaagtaata agcttatgag tcttttgata gaattataag attgaatttg
    26701 aatacattga agcaaattta taaaaagtat tcaatatcag tgataagaaa gaagtgtctc
    26761 aaaatgaact tttcagttca tgaggcgctt tttttatgtg aaattaaatt tattttaatc
    26821 atttcaacaa tgaaaaatat atttatctct attttgagac agaccgttct ttattttttt
    26881 atctttggtt aatttttaaa tatttaagta ggttctaata aaaaactcta tttagttaaa
    26941 tatagttaac ttgatatata ataagataat acatataatt aagtaaaaga ttgtttttta
    27001 gtttatgttt aaatacataa ttacactttg ttaaaaacat aaaattatta ctaaagtaga
    27061 aataggaggt tttagcatga attttaatta taatttgcca gtaaatatct tatttgggag
    27121 cggcagaatt aatgatgtgg gaaaagaagt atctaaatat ggcaaaaaag tattgatagt
    27181 aacaggtaaa aatagtacaa agaaaactgg attacttgat aaaacaatag atttactaaa
    27241 agactctaaa atagaatatg aagtttttga tagagtagaa caaaatcctc ttataactac
    27301 tatatattca ggagtagaaa ttataaaatc aactggatgt gattgtgttt taggtcttgg
    27361 tggtggaagc attatggatg ctgccaagag tatagctttt tcttataaaa acccaggaga
    27421 tttaaatgat tatatatttg gaattagaca aggtgaagaa gctttaccta taatcttagt
    27481 tccaacaaca tcaggtacag gtagtgaagg aaattgtttt gcagtactta caaattcaga
    27541 aactaaagat aaaaaatcta taaagaagaa tagtatgtat gcaaaagcat caataattga
    27601 cccagaactt atggttacaa tgccaaaaca tataattgca tcagttggat ttgatgcact
    27661 tgcccataat atggaagcat atctttctaa tggaagaaac cctcttgcag atgtacaagc
    27721 tatttatggg attgaactta tatcagaaaa cctaataaag gtatataatg atgttaatga
    27781 tttagaagca tgggaaaaaa taacacttgc aagtactatt ggaggaatga cgattggtac
    27841 agcgggtaca gccttgccac atggaatgga gcatcctgta agtggtctta gagatgtaat
    27901 gcatggaaaa ggacttgcag ctttgactcc tactattata gaaagttcat gggatagtga
    27961 tattgaaaga tttggtaata tttcaaaggt attaggagga acttgtgcag aagattgttc
    28021 agatgcaatt agaaatttcc ttaaaaaaat agatttagat ataaagctaa gtgatttagg
    28081 tattgaaaaa tcagatatag attggttaac tgataattgt atgaaaatat cagttgcaaa
    28141 tattaagaga catccaaagt attttaataa ggaacaaata aaggaaatgt accataagtc
    28201 tttataattt gactttacat atttagtttt atataaaaat tataaattaa taataaaaaa
    28261 ttataagtaa ttaatagtaa aaaaattgtg tatagaagtt atatatataa taaatttgtc
    28321 ttaagctttt attttacttt ctaataaata aaggagttta aaaaagggaa gattaattaa
    28381 gtaatatttt ataaaattct tcgatttaaa aactttaagt agaggtgaaa taaatgttta
    28441 atcccaaaag aataatattc gaaaaaggaa ctttagacac tgatgtgggt aaaaaaatat
    28501 acaataaatt tagagataat aaagatgtag agataattaa tatatcatca aataggatta
    28561 aagaacatat tccaggtgaa aatctatatg aacaatatag aagtgggaag cagactatgg
    28621 tagttgggat tagaaaaagt ctgaagtttc aaacatgtaa accttctgct aattatcaac
    28681 taccacttgt cagtggatgt atgggaagat gtgagtattg ttatttgaac acacaattag
    28741 gtgataaacc atttataagg gtgtttgtaa atgtagatga aatacttgaa cagacaaaaa
    28801 aatatattga agatagaaag ccacaggtaa ccatatttga aggggcagct acatcagacc
    28861 cagttccagt agagccatat actaattctt tgaagaaaac aatagagttt tttggaagag
    28921 aggaaaaagg aagatttaga tttgttacta aatacagtga tgtggatagc ttattggatg
    28981 ctgaacataa taatcataca gaaatacgat ttagtatcaa tactcctaga attataaatg
    29041 aacatgagca ttatacagcc tctgcaaata aaagaataga gtcagctgta aaacttgcta
    29101 gggctggtta tcagattggt ttcataatag cacctgtgtt tttatatgaa ggttggaaag
    29161 aagagtataa aaatctgatt ataagtataa aagaagcttt accaaaggat tttgataaaa
    29221 aaattatatt tgaagttata tctcatagat atacaactaa agcaaaaaat agaatactag
    29281 agatttatcc agatacacta ttgccaatgg aaaatgaaga tagaaagttt aaatacggtc
    29341 aatttggata tggtaagtac gtgtatgata aagaacaatt agatgatatg aaagagttct
    29401 ttcttaagga aataaatgga gtatttaaaa atagtttaat aaaatatata atatagcaaa
    29461 tcttacctaa agatttgcta tattttattt ttatatattt tatgtttata ataaagcaat
    29521 gagatttagc attatcatat aagaaaatta aaaagggcac tatctatcaa gaacattttt
    29581 tctagttttg atactataaa ggataagagt aacaaaaata tcaggaggag ttatagtatg
    29641 catgcatggg agtctattca aattacattg gatttaatag aagcaaattt atcagaagaa
    29701 atatcaatta atgaattagc aaataaagca aatttatcac cattttacta tcaaagacta
    29761 tttaaaagat tggtaaataa gactgtaatg gagtatataa agcttcgtcg tcttgcaaga
    29821 gcttctgaat acttagcaga acataagaat cgtatacttg atgtagcttt aaattttgga
    29881 tttgctagtc atgagacatt tacacgttcc tttaaaaaag catatggatt aactccagaa
    29941 aaatatagag caaatccagt aaaactcaat cattttatta agccagaatt aattttaaat
    30001 tatgctatgg ttgatgaagg tgttccactg attgcagatg atatagtgat tgaagtcaat
    30061 cgtaagacat tgagtaatcc aaggacattt gttggaatag aaatagaagt acctatttgc
    30121 cagttggtgg gcggtgaaac aactggaatt gcaatagtag aggaattatg gatgaaactt
    30181 ggaagtcaaa gacataatat accaaatcaa attccaggtg gaaatgaatt tgctgctctt
    30241 tatatgggaa atgcaaaaga aggaaattgt atgtatatgg caggtgtaga ggtagagtca
    30301 ggtacatcag tagaaggata tagtactttt gagttgccag ctaaagaata tttagtatgt
    30361 ggatttgagg cagaaaactt taatgagtta gttaattcag ctgtttttaa agcagataaa
    30421 tttatggaaa gatggatgaa gaaacataat ttaacaacta ctgattttgc aattgagatg
    30481 tattacccca ctacacctga agcagcatat ctggaacatt ggattgttcc ggttcctatt
    30541 gaacaataga aaggatgatg aatatgaatt ggcaagatgc gttttttcaa gatactaaac
    30601 ctacatttga gcaaattaat gaatttatta ataatccatt atgggacaag ttaaataaaa
    30661 ctttggtttc tacttataat gtagaaccta agcttgaata cagtaaatgt tcaatgcaaa
    30721 gaggttggaa tgtaaaatat aaaaaaagag gtaagtcact atgtacttta tatccacaag
    30781 aaggttattt taaagctttg gttattgtaa gtgaaagcaa tagagtagag gttgatttat
    30841 ttattaatac ttgttgtgat tatatcaaaa aaatatataa tgaggtaaac ttttttaatg
    30901 gaagtaaatg gctaatgatt caagtagata gtttattggt attaaacgat atgcttgaac
    30961 taattaaatt tagagcttaa catatagaac aggatgaata tataagtcat cctgttttta
    31021 tattcaatca gataaattaa tatatagtct ataatatgaa taatttagta aatttaaatt
    31081 tactttatct ttctacattc tttgcaacta agtccacatg tgtttttttc ttgcatatta
    31141 gtagcacccc aattagcaaa ttgttgtaaa ataggcacaa tacttttacc tttacttgtt
    31201 aatgtatatt caacttttgg aggaatttca ttatattgtt tacgatgaac aataccagta
    31261 gactctaaat cttttaatga ttgagctagc atagtatttg taatgccatc caattttctt
    31321 tttatgctac tatatctaat tggattttca actgctaaca tacatataat aggtaatttc
    31381 cacttaccac cgacaatgtc taatgcataa cggattggac aggttaattc ttcaacgttt
    31441 ttagttatat ttttttcagt atttatactc attttaatta ctccttagtt atagtagtat
    31501 cttttttata ctttatcaga aaaaactgca tacttgtttt ttctgtattt tatattaaaa
    31561 ttatatcaaa ggataatcct taatacaagt atttaaaagg ggtgtaagaa ttatgaaagc
    31621 attagaattt ttgaacaagg caggagttta ttatttagca acagttgatg agaacaatca
    31681 agcacatgtg cgtcctttag gatttgttat ggaatacaat ggtaaactta cattttgcac
    31741 atctaatcaa aaggatatgt ttaagcaact tatttttaat ccaaaggttg aactttgttg
    31801 tatagaccaa aaccttaata ctctacgtat tttaggtgaa gcagtatttg ttacatcaaa
    31861 agaaacacaa gctaaggcac ttgaaataat gccaagttta ggtcagatgt actctgtagg
    31921 agatggtaag tttgaaatat tctctattga taaggcaaaa gttagttgtt gttcaatgag
    31981 tggtaaaaag attgatgtag agctttaatt ataattgaat cgacatccat agcgaaaatt
    32041 aagaaaacgt tattactgaa atattgatag atttaattta aatgcaataa aaagattttt
    32101 aactaatgaa tatgtaaaaa tttttagtta ttagatgtgt atttcaataa tgatatacta
    32161 ttttgaata
//
LOCUS       DAMJYR010000035        31731 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.035, whole genome shotgun sequence.
ACCESSION   DAMJYR010000035 DAMJYR010000000
VERSION     DAMJYR010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 31731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31731
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.035"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(149..751)
                     /locus_tag="PC471_001622"
     CDS             complement(149..751)
                     /locus_tag="PC471_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 family protein"
                     /protein_id="HDF2788754.1"
                     /translation="MDNLIRILILAVIGGFIGYVTNVVAIRLIFRPIEPIKIPILNIE
                     IVGLIPKRRAEIAANVGEIIQEEFLSMDEILANIITDEDKEEVVRYIKARVKIIIHEK
                     VSFIPSGIKNMIQDYLGEIIESEVKQSIDELSKNIINKANERIDIQKMVEDKINELDL
                     YELEEIIIRIAKKELKHIEFLGLVLGFLIGIAQGLITMFI"
     gene            complement(931..1749)
                     /locus_tag="PC471_001623"
     CDS             complement(931..1749)
                     /locus_tag="PC471_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sporulation protein YtxC"
                     /protein_id="HDF2788755.1"
                     /translation="MVEKDNISSMKFLRNNNVNDRLIYTNDIVEVVIDSAKQEVLNKK
                     ILNGTKAEQRALNIDEISTEITSMIISIMKEKLLKEYILKCYGRTYPEDKNNIYIYSL
                     NIFAKKEIFMRETVFTRVRNYIEENDFINVEGFIRFRMREFMKYISAIGDIAVEEYLI
                     KKDQDEFIRVLKYFIDTQEEKIDLLKVHIMEDNTFVLYDKNGNKIDSIDDEEIINMVI
                     RENLNYEDFLISTLLTLCPKKIEILDLLNNNCSKEIIDTVEAIFENKVSIIMEN"
     gene            complement(1752..2513)
                     /locus_tag="PC471_001624"
     CDS             complement(1752..2513)
                     /locus_tag="PC471_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2788756.1"
                     /translation="MEFTREPFILDDDLCLALVDKRISYLMENELKLRNIEIIKTIEC
                     KELYESIKYHPDICICNLGKGDIIVAPNVYNQYKELLSRYKFNIIKGEANLLNRYPYD
                     IPYNIAIVGDYAIHNFKYTDKAILDYIENNKLKKINIEQGYSKCSICIVDRTSIITSD
                     KGIWKSMKNTEIECLLIEKGHINLFEMNYGFIGGCTGLISKDKLAFCGDVKKHPDYER
                     IKSFVESKNKEIVTLSCENLLDIGSIVPLMTRKER"
     gene            complement(3003..4115)
                     /locus_tag="PC471_001625"
     CDS             complement(3003..4115)
                     /locus_tag="PC471_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HDF2788757.1"
                     /translation="MKIKRILMACFVVVFTIFILAICNFDFGFDKVNKNNSNIDSAKS
                     KQSESKSLLTSAEKDKSNSKSKNKEDDTEDDSYVRKTIGVLLDTENLNNESYNNNVLS
                     ALKRADEDFDVRIKLEKPKTSKSFKESIEKLHKYEPDFIIAVGARFAEPIKEAATKYP
                     KQQFAIVDYTYKVQPANVISVSFQDNVTGYLAGLIAGKMTDTGKVGFIGGVEGSSRDK
                     YESGFRKGLKTSNKDAELLVKYSNIFENPESARTIAKEMRDSDRDVVFSATEDDSKRA
                     IQVARENGGKVIAINKDQYDMAPDNVISSIIKDVEQPVYELIKNVAKDGFKGSKVMEF
                     KIKDGELGLTSKSSRNIPPDVLEYIKKEYNKVKDTY"
     gene            complement(4241..5245)
                     /locus_tag="PC471_001626"
     CDS             complement(4241..5245)
                     /locus_tag="PC471_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HDF2788758.1"
                     /translation="MRFKRILILMLTVAMIAGMLVGCANNPSNNPSSSKDNKSDISDK
                     SNSKKSVSMILDIEGTNNEAMNNSALLALNNAQKKLNIDTNKVESDDSSTFSNSIDIL
                     CNDNYDLIIAVGARFAKPLEMVAKKYPKQQFAIIDYEYDKQPSNITSISYEDNKSGYL
                     AGLIAGKMTESDKVGFIGGTKSSSRDKFESGFREGVKFSNSSIKDISVEYADVFKDSK
                     SVESIAKKMMDNGVDIIFSTTEDDSKAVIDAVRAKNKKVIATNKDQHELAPENVISSI
                     VKDFENPTYNLIQSFVKGNYKGGKVIENTVKSGSTGLAQNSSQNIPPDVLEYVNKNNK
                     "
     gene            complement(5533..6381)
                     /locus_tag="PC471_001627"
     CDS             complement(5533..6381)
                     /locus_tag="PC471_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HDF2788759.1"
                     /translation="MKNLKIICDSLSDVPKDLLDKYDIDIVSLTVILEEREYKDRIDI
                     SGDEFYKRLREENVYPKTSQATYAQFKEIFEKYTNEDREILYIAGSATATGTYQSAVM
                     AKNDVDGKIYTYDSNLLCMGTGLLVIKAGELASEGKSVAEILPVLDELKEKAFVIFSV
                     DTLEYLQKGGRISSTKAAIGSILNIKPILEIRDGLVSQISQVRGKKNVISKMMELIKS
                     NCGDDLSDQIVYIGYSDDFKEKEQLSKVMQDELRPKEIRFFQIGVCIGAHAGPGVTGI
                     ICLKNK"
     gene            complement(6417..6887)
                     /gene="trmL"
                     /locus_tag="PC471_001628"
     CDS             complement(6417..6887)
                     /gene="trmL"
                     /locus_tag="PC471_001628"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HDF2788760.1"
                     /translation="MSLNIVLVEPEIPQNTGNIIRTCAATGSTLHLVKPLGFSLDDKH
                     LKRCGLDYWDIADIQYYDSFEELQEKYPNSKYFFSTTKAKQSHSDVKYEENCFIVFGK
                     ETKGLPEPLLKANMDTCIRVPMLDVEKARSLNLSNSVAIVVYEALRQIGYPNMR"
     gene            complement(7081..8250)
                     /gene="nth"
                     /locus_tag="PC471_001629"
     CDS             complement(7081..8250)
                     /gene="nth"
                     /locus_tag="PC471_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HDF2788761.1"
                     /translation="MKNVNLKEINEKDVILEEEINKKGMDKKNIIEKEEINKKGIDKK
                     DIIEKEEINKKGIDKKDIIEKEEINKKGMNKKDIIEKEEINKKGMNKKDIIEKEEINE
                     KGIDKMIGKKDEIIDEKDIENFMDIEEDKKSIKVSKKSTKKGETEKKTKKSSSNKATQ
                     KTSKETKSKSKIKNESANEKDVNKILDELEKLYPDAKCELNYGTAFELLIATILSAQC
                     TDVRVNKVTSELFKKYNTARDFANLSIEEISKEIKSCGLYKSKSQKIKDTSEQLCELY
                     DGEVPDSLEKLIKLPGVGRKTAGVVLSNAFNHPAIAVDTHVFRVSNRIGIVDEPNPQK
                     TEFALMEAIPKERWSHSHHVLIFHGRRMCKARNPECASCPIKEDCNYYKELNETK"
     gene            complement(8448..10685)
                     /locus_tag="PC471_001630"
     CDS             complement(8448..10685)
                     /locus_tag="PC471_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDF2788762.1"
                     /translation="MKYNNTSEINPLDYILGQERAVEAMELGLKINNPAYNIYIAGEP
                     GTGKSTYALKVLNEYASKKNTHKDWCYIYNFENPREPIIVELEKGFGRELKKDMEKLI
                     ESLLEDFKNAFESENFEIEKNKLLDEYEIEKDMLLKQIKKYGEEKGFKLKQSKMGIVF
                     IPLKEEEDESDESDEEFYKAKRELENMAIQVVYKIRNLEEIAEKAVLELEEEIAKLVV
                     EPHIKRLCEKYENYDKIQTYLENIKKDIIEYMYLFYLDEEELKDKYDKEHFIKYKINL
                     FVDNGSSKNKESAPVVVEINPSPANLFGKAEYDYANGNIKTDFTKLLSGAFHRANGGY
                     LVLYADQLLKYTMSWEILKKTLQTKKVILETQTAIKPENMPLDVKLVLIGSHYIYDIL
                     YRYDEDFEKYFKVFVDFDSEMDKNEDNEEGIARFIAYQCDKNNLRHFTKSAVEEVIKF
                     STRLSGDLEKLSTKFNKIMEVVIEGSAYAEFRNSEYTEQCDVKKAISEKRKRINRVET
                     HMDESIENGFTLIETEGRKVGVINGLSVLSTGEYSFGRASRITATTSPGSKGIVNIER
                     EVNMSGSIHNKGVLILGGYLSENFAQDLLLSLNAYVCFEQNYGGIDGDSASSAELYVL
                     LSSLSGVSIKQNIAVTGSINQKGDIQVVGGISEKIEGFYSICKTKGLNGEQGVIIPRK
                     NQRNLVLSDEVNDAVRDGKFKIYTVERVEEAIEILTDVKFEEIKKLVKEKLISFSKIQ
                     TVSKE"
     gene            complement(10791..11705)
                     /gene="queG"
                     /locus_tag="PC471_001631"
     CDS             complement(10791..11705)
                     /gene="queG"
                     /locus_tag="PC471_001631"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HDF2788763.1"
                     /translation="MDNKELKAFCNLIGLNCVGVAGVDKYDNLEKILKDRQQKGYLTG
                     MEEPIIENRINPRHIMKDANSIIVCAFPYHINRQEKNKNSNLSKYCHGKDYHIVVKDF
                     LQQICDYISKTVTDFKYKLFADNGPLVDRYLAYLSGIGYFGINNNIITDEYGSYVFIG
                     YIVNNYKFEEDKPLEKSCFKCGKCVKYCPGNAILGNYDMNPKKCLSYITQKKGDLSEK
                     EKVVLKNGKKVFGCDICQEVCPHNVEIPTTHILEFKENIIDYLNYDEIQNISNKEFKR
                     KYGDRAFSWRGRNIIKRNMEIILDKYDD"
     gene            12727..13131
                     /locus_tag="PC471_001632"
     CDS             12727..13131
                     /locus_tag="PC471_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HDF2788764.1"
                     /translation="MIKITIPGRPISKSNFKLHNINGQAWMPSKGKYSKYLAYENMIA
                     GFINQQYQGETVEENLITVLKLFFPNKRMGDLHNYPKSICDGIEKSGIIKNDKQLKPV
                     LLFDFIDKDNPRVEIELYPVSKYDISYNIFEK"
     gene            complement(13388..14383)
                     /locus_tag="PC471_001633"
     CDS             complement(13388..14383)
                     /locus_tag="PC471_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HDF2788765.1"
                     /translation="MRNLGNTNMKIKRVGFGGIPIQRITQDDTNLVINELEKQGINFI
                     DSARGYTISEEAIGIAIEGKRDKFFLATKSMSRDYDSMKRDVEISLNNFKTDFIDLYQ
                     FHNVKEEEYDNLFKDKMAYSALLEAKEQGKIKHIGITSHNLNTIEKAIEDGKFDTIQF
                     PYNIVEGQADEVFKKAHEKGIGIIVMKPLAGGALDNATLAIKYILSKEYIDVVIPGME
                     SVEQVRQNVAVLENLVLDEKDNKEIEEIRNSLGKKFCRRCEYCMPCAVGINIPLSFLC
                     EGYYTRYGLKEWAKEKYEVMDVKPTECIDCGLCESRCPYELPIREMLKTVVEKLG"
     gene            complement(14667..15503)
                     /locus_tag="PC471_001634"
     CDS             complement(14667..15503)
                     /locus_tag="PC471_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HDF2788766.1"
                     /translation="MIKLGTHLSIAKGFANAAETAVYIGANTFQFFSRNPRGGNAKEF
                     DEKDIIKFQEIRKENNFAPLLAHAPYTMNLGGTKDDVYEFATRVIDEDIRRMDSLGIE
                     YMCFHPGSHVGGGVDFGIDRIVKGLNNAIKGDENITILLETMSGKGTEIGFKFEQLKS
                     IIDKIEHKEKIGVCLDTCHIFSAGYDIVNDLDGVLEEFDKVIGIERLKTVHLNDSMMP
                     FGDKKDRHAPIGEGKIGLKALIDFMEHPSLKHLPFFLETPFDDEGHKRELKMIKEILS
                     SK"
     gene            complement(15991..16554)
                     /locus_tag="PC471_001635"
     CDS             complement(15991..16554)
                     /locus_tag="PC471_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2788767.1"
                     /translation="MKEVLVLRDLECIKAIAHPRRIDILKAFDKSPLSAKQLSQLLEE
                     PHAKINYHIKMLYKVGILELVEEKIKSGIVEKYYYPSAKNVVIGNRILNFSLDNGEEK
                     EELYISKFENMSEVFYKAAEEDVLENENIVDYHDISLTHDELVELSDTMKSKIDEILN
                     KRQHNVEGSKYDIAMVIVPTLEEECPS"
     gene            complement(16652..17581)
                     /gene="glsA"
                     /locus_tag="PC471_001636"
     CDS             complement(16652..17581)
                     /gene="glsA"
                     /locus_tag="PC471_001636"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HDF2788768.1"
                     /translation="MNTLDETLLKEIISSNKKYTNYGQVASYIPELKNARRNDLGICI
                     IDSENNLYSAGNCSTKFTIQSISKPIVLAMALMDNDWEDVFSNVGMEPSGDPFNSIMK
                     LEINDTKKPCNPMINAGAIVTTSLINGSCLEEKEERMLSFFRKLAKNDNIGINYDVYK
                     SEKMTGDRNRAMAYLLKSDGFIRGNVEDVLDLYFKQCSIEIDSVDLARIGINLANYGV
                     DIENGEHLMSEMVSRIVKTFMMTCGMYDASGEFAIKVGIPAKSGVGGGIMASVPGRMG
                     IGVYGPALDKKGNSVAGVKVLEELSNKLKLNIF"
     gene            complement(18500..20167)
                     /locus_tag="PC471_001637"
     CDS             complement(18500..20167)
                     /locus_tag="PC471_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside kinase"
                     /protein_id="HDF2788769.1"
                     /translation="MKKINLKIEGKDKEYSLEENSPGIRLGDIAKEFCDEHKGYITLA
                     VVDNKLKELNCRVKKDCEINFLDTTNEDGERVYFRVMSFIFVMACREIFWDSRVTIEH
                     SLSDGLYCEVHIDRKLKEADVENIKNKMKEIVNNDYVIEKIEVTRNEAISIFENNEMY
                     EKAELLKYKEYDSAKVYKCRNYIDHFYGYMLPSTGYIKSFDIKLYNGGVIILGPSEED
                     KTLPMKFVPQPKLSSVYYEAEEWSRLMGIDKVVSLNKIIENNEYGDIIRTFEALHEKK
                     LSQIADMIKDGNKRVVLIAAPSSSGKTSFAHRLSIHLRVNNLNPISISLDDYFIDRKH
                     TPLDEYGNYDFESIYAIDLEKFNLDLKKLLAGEEIDDIRFNFKEGKREYTGKKIKIDS
                     NQPIILEGIHGLNPILTSSIPDEDKFKIYISALTQINLDDHNRIPTADLRMIRRIVRD
                     YNFRGYSADNTILQWASVRRGEKKNIFPYQEEADAIFNSACVYELAVLKRFAKPLLEE
                     IKEDNPAHIEATRLLKFLQYFVELDDTSDIPGISILREFIGGSKIVD"
     gene            complement(20241..20996)
                     /locus_tag="PC471_001638"
     CDS             complement(20241..20996)
                     /locus_tag="PC471_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HDF2788770.1"
                     /translation="MAVGIASLLFNIEEALNICESIKQITHLEIGIDNISECSELCKY
                     KERISKLGLSIGIHLPMELNTCENIEYIRNSWINFIEKIEFELKGFDLRYFNLHLGYV
                     MTNRVRKNRDKYLGNSVDFLDKLNTNSYVCIENTYSKGGDFSNIGNISYDFEYIFKRI
                     KNSKICFCYDTGHCLIDEDAYVKNLKDKIRLIHLSDNDGINDTHVGIGRGILSEEGIK
                     EILTLDAEYLVLEINYEDIEDTISKLNNIVGEG"
     gene            complement(21006..21542)
                     /gene="lepB"
                     /locus_tag="PC471_001639"
     CDS             complement(21006..21542)
                     /gene="lepB"
                     /locus_tag="PC471_001639"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HDF2788771.1"
                     /translation="MNETIKEEIVEWIKIIITALFFAFIITRFIKPTLVNGESMYPTL
                     KSHDYLVANRMTYKLSEPKCGDIMIFKTDLLQENGRKKELVKRVIGVPGDHLKIKDSK
                     VYINGKLLNEVSYIHDNYTEGDIDMVIPKGKVFAMGDNREVSLDSRYKEVGLVDEENI
                     KGKVILRVFPFTDIGIFE"
     gene            complement(21708..22244)
                     /gene="lepB"
                     /locus_tag="PC471_001640"
     CDS             complement(21708..22244)
                     /gene="lepB"
                     /locus_tag="PC471_001640"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HDF2788772.1"
                     /translation="MGEAVKKEVVEWIKVIVIALVLAFAITRFIVPTIVKGESMYPTL
                     VERDYLIVNRIAYKVGEPKYKDIIVFKTDLTEENGKKKDLVKRVIGVPGDHVKIQDSK
                     VYVNDKLLDETSYIHNNRTDGDIDIVVPEGKLFAMGDNREKSLDSRYDEVGLVDEHTI
                     LGKVLVRLYPFSKIGTID"
     gene            complement(22344..23654)
                     /locus_tag="PC471_001641"
     CDS             complement(22344..23654)
                     /locus_tag="PC471_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HDF2788773.1"
                     /translation="MTELPKEFLEDIEEILKDEYDDFIKSYEESKTTGLRINTLKIDK
                     KKLFDLELFDLNQIPWAEEGFYYDDKIDRPGRNPLHEAGAYYLQEPSAMSVIPKADIK
                     EGEKVLDMCAAPGGKSTYILSKLNDTGLLVSNEINASRIKALGENLERFGARNCIITN
                     TDSNSLRKTFNGYFDKVIIDAPCSGQGMFRKDEVAISDWSYAKVVECQSIQKEIIRDG
                     YKMLKKDGILIYSTCTFSREENEHVINEFISEYPHAELIEMERLWPHKIKGEGHFVAK
                     IKKLEEEECNVKELKIKNLDKEIKDYREFEKKFLKVDFYSNKKNKFYLRGENLYLLPE
                     IYPDTKKLKVLRYGLHLGVLKKNRFEPSHALSHYLKVEDVKNVENFSVQSESILKYLK
                     GDVVNSNDSRGWVLVSVEGIPLGWGKESSGVIKNHYPKGLRKVF"
     gene            complement(23733..24449)
                     /locus_tag="PC471_001642"
     CDS             complement(23733..24449)
                     /locus_tag="PC471_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family intramembrane metalloprotease"
                     /protein_id="HDF2788774.1"
                     /translation="MKLDLFLLAIIPILIGMFWIRSKDRYCREPLIHLIKFFLIGAFL
                     SVIIILLENLLMKFNVFEGYSELIYVSFVVAGLVEEGVKALILIPALIKEKHFTEKLD
                     GIIYSVFLALGFATIENMVYIFSESRNLALQVGINRAVISIPAHVMFAITMGYYISKY
                     KFEGNKNKRREYLFMAVLIPILLHGVFDFILMIEYRWAIILLIVYVIILWKINLDKLE
                     KYMNHSKKVFFGNLRKKKKK"
     gene            24694..25965
                     /gene="sleC"
                     /locus_tag="PC471_001643"
     CDS             24694..25965
                     /gene="sleC"
                     /locus_tag="PC471_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore cortex-lytic protein SleC"
                     /protein_id="HDF2788775.1"
                     /translation="MQDGFLTVSIIDATNNRPIQNAVVNIYSMSNGSQSSSTLYQNLR
                     SNESGQVTGLVLPAPDVDYSLQPSDVRPYSQYIVEAIADGYETVVIEGTQLLATIEAR
                     QGVPMSPRTRSKRSFSRQSELIFDIGEHTLYGTYPPKIPESNLKPLPPPTGFVVLDNP
                     VVPEFIVVHDGLPEDSSAPNYWIPFKEYIKNIASSEIYSTWPEQTIYANVIAIISFTL
                     NRVFTEWYRNKGYNFTITSTTAYDHKFINNRNLFEPINVVVDAIFNTFIKRPPTSRQP
                     LLAQYCDGQKSQCPDQMTQWGSKDLGDQGYDYESILRYFYGDEIVFERAPIVSGVPVS
                     FPGTTLQVGSSGQYVRTIQNQLNAISNSYPAVPKVIEDGIYGTDTENAVKIFQGIFGL
                     PQSGVVDFKTWYEISRVYVATTRIASLNPLI"
     gene            complement(26391..27047)
                     /locus_tag="PC471_001644"
     CDS             complement(26391..27047)
                     /locus_tag="PC471_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2788776.1"
                     /translation="MKKGDFIWSGVLICIIAVLAIPSSREVFMEATGAHPYIGGFLKF
                     AVLATMGDLLGVRILYKDWKIPVGVFYRAIVWGIIGVVTTYGMSIYSSGVGQAQVDGM
                     LPFEGIAFANALFKSATMNILMSPTVFLFHKVMDCFIDKKYEVGKGIKVKLKDVVNIV
                     DWNAYLGFSVLKTIPFFWIPCHTIVFLFPAEYRVIASAFASIALGLILALANKSKVAV
                     "
     gene            complement(28159..28569)
                     /locus_tag="PC471_001645"
     CDS             complement(28159..28569)
                     /locus_tag="PC471_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2788777.1"
                     /translation="MKIKKSLISAMMVSAMVLGGTGAVFANGNDSSNIALPKFESLIK
                     SGITDPTELEKYGVEHYTYEEYAKHIQELKDYAKDPNAVKDVSQKDLEETIKKMEQEL
                     EKIKTEGLKIMKPITIENEDGSQISIAYNYAAID"
     gene            complement(28785..30167)
                     /locus_tag="PC471_001646"
     CDS             complement(28785..30167)
                     /locus_tag="PC471_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HDF2788778.1"
                     /translation="MFIKLLEMSFTSGVLLLIVIFIRACLFKKLPKKVFVLLWLIVLF
                     KLLIPVSLNSGFSIYSIIESTFDVELGNTSTENYTLKKASDSTGKNNSDEFEIMSNIE
                     NIMMLEMLGILSFIVTIMFSILLCFRYFKIINLFKTSIHIKNHEFIKKWMCKNSRFRR
                     FIVCISEFTRTPLTSGILKPRIILPKQMNYANNQTLDFVLTHEMVHINSFDNLLKLFM
                     LVALVVHWFNPLVWVMFFLLNKDIEFSCDEKVMSILGEEYRADYATTLISLAEENNPF
                     SMTICSGFGKTKIEKRIVNIMKFKKSTYVTFLVSTVLVCGTGLVFATENKEINEVESF
                     GLTKNEQSDKIGFSISSKSTDLYSPKVESDILVSKAKSDGETHFSTAKVSVIEFREHE
                     ESSMSKVTNSENSEETYVNKSSNSKDVLYTDSGEVSIERSGYNDNRFVILRKGNLLAV
                     NKEQYRTWIE"
     gene            complement(30178..30537)
                     /locus_tag="PC471_001647"
     CDS             complement(30178..30537)
                     /locus_tag="PC471_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HDF2788779.1"
                     /translation="MEIKLFDSELKVMEVLWERGESKAGELVKILKEETGWNRNTTYT
                     VIKKLVSKGAIERIEPNFVCKALISKEEVQKQETETLINKMFGGSSEKFLSAFINKEN
                     LSSDEITKLKQIVEKLK"
     gene            complement(30742..31521)
                     /locus_tag="PC471_001648"
     CDS             complement(30742..31521)
                     /locus_tag="PC471_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HDF2788780.1"
                     /translation="MVSRQELKRVSKAQLSGNWGICAISMAVYIALIVLISIVGLPFE
                     PLTTIVVAVIAFCVSAGFTCMFLKITKDEKVKIGDIFVSGRIYLRSFGFYLFESIIVY
                     IYSFIITIIGAFLSFGVLLTSGAIEYLSSDNVGIGTIISTGITLVLIILVLMLPVYIL
                     LLYVSQATFLICEDKDNMGVFKSMGASLDMMKGNVLKLFVLNFSFIGWYILSIATLGI
                     GLLWLIPYVGVTNCNFYRQLVKENPDVEDILLDKEKSYTMY"
BASE COUNT        10347 a         5791 c         2957 g        12636 t
ORIGIN      
        1 taggtaaata caattaatat ctaaatgcta ttaataagta atttactcaa ataggtaaat
       61 acaattaata tctaaatgtt attaataagt aattcactca aataggtaaa tataattagt
      121 acttaaataa atgctacttc tcatatattt aaataaacat agtaattaac ccttgagcta
      181 tacctattaa aaaccctaag acaagcccca aaaactctat atgttttaat tctttcttag
      241 ctattcttat tattatctct tccaattcat ataaatctag ttcattaatc ttatcttcga
      301 ccattttttg tatatctata cgttcatttg ctttatttat tatattttta ctcaattcat
      361 ctatactttg cttaacttca gactcaatta tctctcctag ataatcttgt atcatgtttt
      421 ttataccact cgggataaat gaaactttct catgtattat aatttttacc cttgctttta
      481 tgtatcttac aacttcttcc ttatcttcgt cagtaatgat atttgcaagt atttcgtcca
      541 tagaaagaaa ttcttcttga attatttctc ctacatttgc tgctatttct gctcttctct
      601 ttggtataag acctactatt tctatattta aaataggtat tttaataggc tctattggtc
      661 taaatatcag tcttatagca actacattcg taacataacc tataaatcca ccaataacag
      721 ctaatatcag tatcctaatt aaattatcca ttataaaatt actccttatc aattgtatat
      781 ttgccagcta tattgcatta atcaagtaga tattctttat acttaataac tctagttata
      841 ctatagcttt atatacaatt ataagtcttt tgtgtcgatt taaaaagaat ttttttaatt
      901 ttatatggat agcttttata tgcttagatt ctagttttcc ataattatac taactttatt
      961 ttcaaaaatt gcttctacag tatctataat ttcctttgag caattattat ttaacaaatc
     1021 caatatttct atttttttgg gacatagagt taataaggta cttattaaaa agtcctcata
     1081 atttaaattt tctcttatta ccatatttat aatttcttca tcatctatac tgtctatctt
     1141 atttccattt ttatcgtata aaacaaatgt attatcttcc attatatgaa ctttaagcaa
     1201 atcaatcttt tcttcttgag tatctataaa gtattttaaa actcttataa attcatcttg
     1261 gtctttcttt attaaatact cctcaactgc aatatcacct atagcagaaa tatatttcat
     1321 aaattctctc attctaaatc taataaaacc ttctacattt ataaaatcat tttcttctat
     1381 ataatttctc accctagtaa atactgtttc tctcataaaa atctcttttt tagcaaatat
     1441 gttcagtgaa tatatgtata tattattttt atcttcagga tatgtacgcc catagcattt
     1501 tagaatgtat tctttcaaaa gtttctcttt cattatactt attatcatac tagttatttc
     1561 cgttgatatc tcatcaatat ttaaagctct ttgttcagct ttagtaccat taagtatttt
     1621 tttatttaac acttcttgct ttgcagaatc tatcactact tctacaatgt cattcgtata
     1681 tattaatcta tcgtttacat tattattcct caaaaatttc atggaagaaa tattatcctt
     1741 ctcaaccaaa gttatcgctc ctttcttgtc atgagaggaa ctatagaacc tatatctagt
     1801 aaattttcac agctaagtgt aactatctcc ttgtttttac tttctacaaa actttttata
     1861 cgttcatagt caggatgttt tttaacatct ccacaaaaag ctagtttatc cttagaaatc
     1921 aaccctgtac atccacctat aaatccataa ttcatctcaa ataaatttat gtgacctttt
     1981 tctatcaata aacactctat ttctgtattc ttcatacttt tccaaatacc tttatctgat
     2041 gttattatag acgttctatc tactatacag attgagcatt tagaatagcc ctgctctata
     2101 tttatctttt taagtttatt attttcaata taatctaata ttgctttgtc agtatactta
     2161 aaattgtgta tagcatagtc accaactatc gcaatattgt aaggtatatc ataagggtat
     2221 ctatttaata aattagcttc tcccttaatt atattaaatt tatatctact taataactct
     2281 ttgtattgat tatatacatt cggcgcaaca attatatctc cttttcctaa attacaaata
     2341 catatatccg ggtgatattt tatgctttca tataattctt tacattctat tgtttttatt
     2401 atctctatat ttcttaattt taattcattt tccattagat aactaattct tttgtccacc
     2461 aaagctagac acaaatcgtc atctagtata aatggttctc ttgtaaattc cattttttac
     2521 tcctttttct tttttttgat aaatttttta tgtgaatata aataaagtgt gtagcagaat
     2581 ttttctgccc acacacttat tgttataatt ttatatagtt attctaagct taattagtat
     2641 agctttgtac agttggttca aatttaataa atatatcttt ttatcactaa tctaaactta
     2701 agcagtatat tccgtacagt tagcacaaat ttaataaata tatcttttta ttgctaatct
     2761 aaactcaatc aatatatttt gtacagttag actaaattta ataaatatat ccttttatca
     2821 ccaatctaaa cttaagcagt atattccata ctgttaacac aaatttaata aatatatctt
     2881 tttatcacta atctaaactt aatcaatata ttttgtacag ttagcacaaa tttaataaat
     2941 atatcttttt attgctaatc taaacttaag caatatattt ttataatctt ctacatcaat
     3001 aattaatatg tatctttcac tttgttatac tcttttttta tgtattccaa aacatctggt
     3061 ggtatatttc tactgctctt tgaagtcaag ccaagttccc catcttttat tttaaattcc
     3121 attactttac ttcccttaaa accatctttt gcaacgttct ttatcagttc atatacaggt
     3181 tgttctacat cttttataat tgaacttatt acattatctg gagccatatc gtattggtct
     3241 ttattaatag ctataacttt accaccattt tctcttgcta cctgaatagc tcttttacta
     3301 tcatcttctg tagctgaaaa tacaacatct ctatcagaat ctctcatttc ctttgcaata
     3361 gttctagctg attcagggtt ttcaaatata tttgaatact taactagtaa ctcagcatct
     3421 ttatttgaag ttttaagccc ctttctaaat ccagattcat acttatctct actagaaccc
     3481 tcaactcctc ctataaaacc gactttccca gtatcagtca ttttacctgc aataagtcct
     3541 gctaaatatc cagtaacatt atcttgaaat gatacagaaa ttacattagc tggctgaact
     3601 ttataagtat agtcaactat tgcaaattgc tgtttgggat atttagtagc agcttcttta
     3661 attggctctg caaatctggc tccaactgct attataaaat ctggttcata cttatgcagt
     3721 ttttctatac tctctttaaa acttttagaa gtctttggtt tttcaagttt tatcctaaca
     3781 tcaaaatctt cgtcagccct ttttaaggct gatagtacat tgttattata tgattcgtta
     3841 tttaagtttt cggtatctaa caatactcca atagtttttc ttacatatga atcatcttct
     3901 gtgtcatctt ctttattctt acttttacta tttgatttat ccttttctgc tgaagttagc
     3961 aaacttttag actcactctg tttcgattta gcactatcta tattagaatt attcttattt
     4021 actttatcaa acccaaaatc aaaattacat atagctaata taaaaattgt aaaaactact
     4081 acaaagcaag ccatcaatat cctttttatt ttcacaaaat tcactccctg atttagatag
     4141 gtctgaccta cctctgcata tgtctttcta tatatgtcta ttataacatc caattcacct
     4201 cataactaaa ctcataagta ttcttggata ttatagtaga ttatttattg ttcttattaa
     4261 catattctaa cacatctgga ggaatatttt gagaactatt ttgggcaagt ccagtacttc
     4321 cagacttaac tgtgttttct ataacctttc cgcctttata atttcctttt acaaaacttt
     4381 gtatcagatt ataagtagga ttctcaaaat cttttacaat agaacttata acattttctg
     4441 gagctaactc atgttggtct ttattcgttg ctataacttt tttatttttt gcccttactg
     4501 catctattac agcttttgaa tcatcttcag tagtagaaaa aataatatcc actccattat
     4561 ccatcatttt tttagctata gattctactg atttgctatc tttaaaaaca tctgcatact
     4621 caactgaaat atcctttata ctactattag aaaatttaac accctctcta aaacctgatt
     4681 caaatttatc tctactagaa ctctttgttc ctcctataaa accaacctta tcgctttcag
     4741 tcatttttcc agcaattaat ccagctaggt atccagattt attatcttca taagaaattg
     4801 aagttatatt agaaggttgc ttgtcatact catagtctat aattgcaaat tgttgctttg
     4861 gatatttctt ggctaccatc tccaatggtt ttgcaaatct agctcctaca gctataatta
     4921 aatcgtaatt gtcattacat aaaatatcta tactattaga aaaagttgag gaatcatctg
     4981 attccacttt gtttgtatct atattaagtt tcttttgagc attgtttaat gctaataaag
     5041 ctgaattatt catagcttca ttatttgttc cttcaatatc tagtatcatt gatacagatt
     5101 tttttgaatt acttttatcg cttatatcac ttttattatc tttgctagag ctaggattat
     5161 tacttgggtt attagcacac ccaaccaaca ttccagctat cattgctaca gttaacatca
     5221 atatcaatat tcttttgaaa cgcattaaat ttcactcctc atacatttac gatattttta
     5281 tatcctattt acttttacat cctatttata atttttttat atacaattta caagtacatt
     5341 ttgaaatttt tttaataaat cttactataa aaaagtgtgc aatagattct tatctattta
     5401 cacacttccc tacaaactaa atctaatata tcaacactta atttcatcca aaataaatcc
     5461 aatttaacat ctcaacaatt aatattctat cctaatttaa taaaaaaata aataatgtca
     5521 tccaaaaata tattatttat tttttaagca tatgatacca gtaacaccag gaccagcatg
     5581 tgcacctata cacacaccaa tttggaaaaa tctaatttct ttaggtctta attcatcttg
     5641 cataacctta gataattgtt ctttttcctt aaaatcatca ctatatccta tatacacaat
     5701 ttggtctgat aaatcatcac cacaatttga ttttatcaat tccatcatct tagaaattac
     5761 attcttcttt cctcttactt gagatatttg cgaaactaat ccatctctta tttcaagaat
     5821 cggttttata tttaatatac ttcctattgc tgccttagta gatgatattc tacctccctt
     5881 ttgtaaatat tccaatgtat ctactgaaaa tataacaaaa gccttctctt ttaattcatc
     5941 caaaacaggc aatatttcag caacagactt tccttcactt gcaagttctc ctgcttttat
     6001 tacaagtaaa ccagttccca tacaaagcaa gttagagtca tatgtatata ttttaccatc
     6061 tacatcattc tttgccatta cagcactttg atacgtacct gtagctgtgg cagaccctgc
     6121 tatataaagt atctctctat cctcatttgt atatttctca aaaatttcct taaattgggc
     6181 ataagtagct tgagaagttt ttggatatac attttcttct ctaagccttt tataaaattc
     6241 atctccagat atatcaattc tatccttgta ttctctttcc tccagtataa ctgttaatga
     6301 aactatatct atatcatatt tatctaacaa atcttttggc acgtccgata aactgtcaca
     6361 gattatcttt agatttttca ttaagtatgc tcctttctca tttaggcttg ttgaaactac
     6421 ctcatatttg ggtatcctat ttgtcttaaa gcctcgtaca ctacaatagc aactgaattt
     6481 gataaattta atgacctagc tttttcaaca tccaacatag gcactctaat acaagtatcc
     6541 atatttgctt tcaacaaagg ttctggtaat ccctttgttt ctttcccaaa tactataaaa
     6601 caattttctt catatttcac atctgaatgt gactgtttag cctttgttgt agaaaaaaag
     6661 tatttactat ttggatactt ttcttgtaat tcttcaaagc tatcgtaata ttgtatatca
     6721 gctatatccc aatagtcaag cccacatctt tttaggtgtt tatcatccaa tgaaaaaccc
     6781 agtggtttta ctaaatgtaa agttgaacct gttgctgcac aggttcttat tatatttcct
     6841 gtattttgag gtatttctgg ttctactaat actatattta atgacatttt aatcctccct
     6901 aagtatcgct tttatttatc aaattttatt ttataaacag aataacaata atattatact
     6961 attatataaa ataatgttaa taataatttt tatttcatgt atataatagt tttttatcta
     7021 acattttcac aagtattatt atatcagaaa atccctcttg tacaacttac tttctaaaag
     7081 ttatttcgtt tcattaagtt ctttataata attacaatct tcttttattg ggcaacttgc
     7141 acattctgga tttctagctt tacacattct tcttccatga aaaattaata catgatgtga
     7201 gtgtgaccat ctttcttttg gtatagcttc cataagtgca aattctgttt tttgaggatt
     7261 tggctcgtcc actattccta ttctattgct aactctaaat acatgagtat ctacagctat
     7321 tgctggatga ttaaaagcgt tactaagtac aacccctgcc gtttttcttc caacaccagg
     7381 taattttata agcttttcta agctatcagg tacttcacca tcatataatt cacatagctg
     7441 ttcagatgta tcctttattt tttgagactt gcttttgtaa agaccacaac tctttatctc
     7501 tttactaatt tcttcaatgc tcaaattagc aaagtctcta gctgtgttgt actttttaaa
     7561 caactctgaa gtaactttat tcactcttac atcagtacat tgtgctgata aaatagttgc
     7621 tattaaaagt tcaaaggctg tcccataatt taactcacat ttagcatctg gatacaattt
     7681 ttctaactcg tctaaaatct tatttacgtc tttttcattt gctgactcat ttttaatttt
     7741 agacttactt ttagtttctt tacttgtttt ttgtgtagcc ttattagaac tactcttctt
     7801 tgtttttttc tccgtttcac cttttttagt acttttttta cttactttga tacttttttt
     7861 atcttcttca atatccataa aattctctat atctttctca tcaattatct catctttttt
     7921 accaatcatt ttatctattc ctttttcatt tatctcttcc ttctcaatta tatctttctt
     7981 attcattcct tttttattta tctcttcctt ctcaattata tctttcttat tcattccttt
     8041 tttatttatc tcttccttct caattatatc tttcttatct attccttttt tatttatctc
     8101 ttccttctca attatatctt tcttatctat tcctttttta tttatctctt ccttctcaat
     8161 tatatttttc ttatccattc cttttttatt tatctcttct tccaaaatta catctttttc
     8221 atttatctct tttagattta catttttcat aattatcccc cttcaaaata ccaatttata
     8281 aaacgctatt tataaatagt ttaatcatat caacaaaaaa ataaacatca atatataaaa
     8341 catacaaaat aagaatttaa tttatatata tttacaaaaa aagcaaaaac ctattaaagt
     8401 tcttgctttt tattatacat aaatacccat taaattctat aataaactta ttcttttgac
     8461 acagtttgta tcttgctgaa agatattaat ttttctttta ccaatttctt tatttcttca
     8521 aatttaacat ctgtcaatat ctctatagcc tcttctactc tttcaacagt atatatctta
     8581 aattttccat ctcgcactgc atcattaact tcatctgata aaacgagatt tctttgattt
     8641 tttctaggaa ttatgacgcc ttgctcacca tttaaccctt tagttttaca tatagaataa
     8701 aatccttcta tcttttcact tataccacca actacttgta tatcaccctt ttgatttata
     8761 gaacctgtaa cagcaatatt ttgtttaatt gatactccac taagagatga taatagtaca
     8821 tataattctg cagaagatgc actatctcca tctattccac cataattttg ttcaaaacaa
     8881 acatatgcat ttagtgacaa tagtaaatcc tgtgcaaaat tctcagaaag atagccacct
     8941 aatataagta ctcctttgtt atgtatagag ccactcatat ttacttctct ttctatattt
     9001 actatgcctt tacttcctgg gctagtggtc gctgtaatcc ttgaagctct accaaaagag
     9061 tattctcctg tacttaagac tgacaaacca tttatcactc ctacttttct tccctctgtt
     9121 tctattagag taaatccatt ttcaattgat tcatccatat gtgtttccac tctattgatt
     9181 ctttttctct tttcacttat tgctttttta acatcacact gctctgtata ctccgaattt
     9241 ctaaactctg cataagcact tccttctatt acaacttcca ttattttatt aaacttagtt
     9301 gataattttt ctaaatcacc agataatcta gtgctaaatt taataacctc ttcaactgct
     9361 gatttagtaa aatgccttaa attattctta tcacattggt atgctataaa tcttgctatt
     9421 ccttcttcat tatcttcatt tttatccatt tcactatcaa agtctacaaa tactttaaaa
     9481 tatttttcaa aatcttcatc atatctatat aaaatatcat atatatagtg gcttccaatc
     9541 agtactaatt taacatccaa tggcatattt tctggtttaa ttgctgtttg tgtttccaat
     9601 attacttttt tagtctgcaa agtcttttta agaatttccc aagacattgt atattttagt
     9661 aattggtctg cataaagtac taaataacca ccattagccc tatgaaatgc tcctgataat
     9721 agttttgtga aatcagtctt tatatttcca tttgcataat catactctgc ctttccaaat
     9781 agatttgctg gacttgggtt tatctctact acaacaggtg ctgattcttt attctttgag
     9841 cttccattgt ctacaaatag gttaatttta tatttaataa aatgctcttt atcatactta
     9901 tcttttaact cctcttcatc taaatagaat aaatacatat attctattat atctttcttt
     9961 atattttcca gataagtttg aatcttatca taattttcat atttttcaca caatctcttg
    10021 atatgaggtt ctactacaag tttagctatc tcttcttcaa gctctaacac tgctttttca
    10081 gctatttctt ctaagtttct aattttatat acgacctgta tcgccatatt ttctagttct
    10141 cttttagctt tgtaaaactc ttcatcagac tcatcacttt catcttcctc ttctttcaaa
    10201 gggataaaaa ctattcccat tttactctgt tttaatttaa atcctttttc ttctccatat
    10261 tttttaattt gcttcaaaag catatctttt tctatttcat attcatccag aagtttattt
    10321 ttttctattt caaaattttc actttcaaac gcatttttaa aatcttctaa aagactttca
    10381 attaatttct ccatatcttt cttaagttct cttccaaacc ccttttctaa ttcaactata
    10441 ataggctctc tagggttttc aaaattatat atataacacc aatctttatg cgtatttttt
    10501 ttggatgcat actcatttaa aacctttaag gcataagtac ttttaccagt tccaggttca
    10561 ccagctatat atatgttgta cgctggatta tttattttca accctaattc catagcttct
    10621 acagctcttt cttgacccaa tatataatct agtggattta tttcagatgt attattatat
    10681 ttcaatattc ccccacctcc ccatatttaa ctttaatgat ataatatgac ttttacacaa
    10741 caagtgttaa agtaaataca cattaaagta tatgaaacag actcttatta ttagtcatca
    10801 tacttatcta aaattatttc catatttctc tttattatat ttcttcctct ccagctaaaa
    10861 gctctatctc catatttcct tttaaattct ttatttgaaa tattttgtat ctcatcataa
    10921 ttcaaataat ctattatatt ttctttaaat tccaaaatat gagttgttgg tatttcaaca
    10981 ttatgtggac atacttcttg acaaatatcg catccaaata cctttttgcc attctttaaa
    11041 acaacttttt ctttctctga caaatctcct tttttttgag ttatatatga taaacatttt
    11101 ttggggttca tatcataatt tcccaatata gcatttcccg gacaatattt tacacacttt
    11161 ccacatttaa aacaactttt ttctagtggt ttatcttctt caaatttata attatttact
    11221 atatacccta taaatacgta agaaccatat tcatcagtta tgatattatt atttattcca
    11281 aaatatccta ttcctgataa ataagctaaa tacctgtcaa ctaatggtcc attatcagca
    11341 aataacttgt atttaaaatc tgttactgtt ttagaaatat aatcacaaat ttgttgtaaa
    11401 aagtccttta ctacaatgtg ataatccttt ccatgacaat attttgataa atttgaattt
    11461 ttattctttt cttgtctatt tatatgatat ggaaatgcac agactattat agagttagca
    11521 tccttcatta tatgtcttgg gttaattctg ttctcaatta ttggttcttc cataccagtt
    11581 aaataacctt tttgttgtct gtccttaagt atcttttcta aattatcata cttatctacc
    11641 cctgctactc ctacacagtt taatcctatt aaattacaaa atgcttttaa ttccttatta
    11701 tccataaaat tattcctttt taatctaatt gactttttaa atttatttaa tttttttagt
    11761 ctttttaacc ttttctagct actaactctt ttaaatactt aatcttttta gctattcaat
    11821 tttcttacct acttaaatct tttagatact tcgtcttttt acctatttaa ttttttacct
    11881 actaacgctt ttagatactt aatcttttta tctatttagt tttttaccta ctaactcttt
    11941 tagatacttc atctttttta tttactaact cttttagata cttcgtcttt ttcatttact
    12001 tatttaactt ttttatctac ttaactcctt taaataatta gtctttttat ctatttaatt
    12061 attttaccta ctaactcttt tgaatactta gtctttttta tttatttaat ttttttatct
    12121 actaacgctt ttagatactt aatcttttta tctatttaat ttttttatct actaactcct
    12181 ttatatactt agcctttttc atttacttat ttaactacta actcttttag atacttagcc
    12241 tttttcattt acttatttaa cttttttacc tacttaactc ttttagatac ttagcctttt
    12301 taatctattc aacctttttt acccacttaa ccttcttacc ttttaatata aactctaaaa
    12361 cttcaaatat cattagttat attttgacat tctttctata gttaagcaaa aagattgatt
    12421 ttttcttata attaatatcc catttacatt gccatttatt aaatctttac ttgacttata
    12481 tggatttctt tttactctct ttactaaact atacttatta atattatgaa ggtcttataa
    12541 tagactaata tgaatatgtc catcatatat caaattttaa aatttgtaag caaaatactt
    12601 atcttatatt actacatcta aagtggaaaa ttttacaaaa aagctctgta aaaacacttt
    12661 aaataacaat ttgcttttaa atcatataca gattataata aatactgtat aaggatggtg
    12721 aaaaatttga ttaaaataac aattccaggt agacctatta gtaaatctaa ttttaagctt
    12781 cataacataa atggtcaagc atggatgcct tcaaagggaa aatattctaa gtatcttgca
    12841 tacgaaaaca tgattgcagg ttttataaac caacagtatc aaggggaaac tgtcgaagaa
    12901 aatctaataa cagtcttgaa attattcttc ccaaataaga gaatggggga tttacataat
    12961 tatccaaaaa gtatttgtga tggtattgaa aaaagtggaa taataaaaaa tgataaacaa
    13021 cttaagccag tacttttatt tgattttata gataaagata accctagggt tgaaattgaa
    13081 ctttatccag tatctaaata cgatatatcc tataatattt ttgaaaaata aagattttat
    13141 gtgttattcg acattatatt gatactccag tatttgaaac tatactaata caacaataat
    13201 aattgtgtta tgtctatact caaacaaatg tttttaaaaa gcattaagta aatatttaat
    13261 aagtattaaa caaatattaa atttatataa tagctataac tatgtaaatt taatatttgt
    13321 atataagacc acatctaata aaaagaaatc ggagtaaatt taatacaccg atttcaataa
    13381 ataaaatcta tcctagcttt tcaacaacag tcttaagcat ttctcttata ggaagttcat
    13441 atggacatct actctcgcaa agcccacagt caatacactc agtaggttta acatccataa
    13501 cctcatattt ttctttagcc cactctttta atccatatct agtataataa ccttcacata
    13561 aaaaactcaa tggtatattt attcctacag cacaaggcat acaatattcg catcttctac
    13621 agaatttttt acctaaagaa tttcttattt cttcaatttc cttattatct ttttcatcta
    13681 gcacaagatt ttcaagtaca gcgacatttt gtctaacctg ttcaacactc tccatacctg
    13741 gtataactac atctatgtac tctttagata gtatatattt aatagccagt gttgcattgt
    13801 ctaaagcacc tccagctaaa ggtttcatta ctattatccc aattcctttt tcatgagctt
    13861 ttttaaacac ttcatctgct tgtccttcaa ctatattata agggaattgt atagtgtcaa
    13921 attttccatc ttctatagct ttttctatag tatttaaatt gtgactagtt ataccaatat
    13981 gttttatctt accttgctcc tttgcctcta aaagggcact ataagccatt ttatctttaa
    14041 aaagattatc atactcctct tctttaacat tatggaattg atataaatct ataaaatcag
    14101 ttttaaaatt atttaaactt atttcaacat ctctcttcat tgaatcataa tctctggaca
    14161 tacttttagt agctaagaaa aatttatctc tttttccttc aatggcaatt ccgattgctt
    14221 cctcacttat tgtataccct cttgcactgt caataaagtt tattccttgt ttttctaact
    14281 catttataac taagttagta tcatcttgag taatcctttg tattggaatg cccccaaagc
    14341 caactctctt tattttcatg ttagtattgc ctaaatttct catatctttc cccacctaac
    14401 ttttaataat tttttatata atataaatat gtcaccttta attatacaac aaattatgag
    14461 aatctttgca atgtatttac atactattca taatataatt atattttata tcagtctatt
    14521 cataatataa tcatacttta tacccagtta atttgtatac aattttcgtt attcctatcc
    14581 attaatttaa attatcacat ttcatgattg tatctccacc ctaattatgt tgagggtgga
    14641 gtatttcaca tatttttaat aaaggtttat ttactagata gtatttcttt tatcatttta
    14701 agctctctct tgtgaccttc atcatcaaaa ggagtttcta agaaaaatgg taaatgtttt
    14761 agactaggat gttccataaa atcaattaat gcttttaatc ctatctttcc ttcccctata
    14821 ggagcatgtc tgtctttttt gtctccaaat ggcatcatac tatcatttag atggacagtt
    14881 ttcaatcttt ctatgcctat aaccttatca aattcttcca aaaccccatc taaatcattc
    14941 actatatcat aaccagctga aaaaatatga catgtatcaa gacacacacc tattttttct
    15001 ttatgctcaa ttttgtctat tatacttttt aattgctcaa atttaaaacc tatttcagtt
    15061 ccctttcccg acatagtttc aagaagtata gttatatttt catcaccctt tatggcattg
    15121 ttcaatcctt ttactattct atctatgcca aaatctacac caccacctac atgacttcca
    15181 ggatgaaaac acatgtattc aattcctaat gagtccatcc ttcttatatc ctcatctatc
    15241 acccttgttg caaattcgta tacatcatcc tttgtaccac ccagattcat agtatatggt
    15301 gcatgtgcaa gcagtggtgc aaaattattt tcttttctaa tttcttgaaa tttaattata
    15361 tctttttcat caaattcttt agcatttcct cctcttggat ttctactaaa aaattgaaat
    15421 gtatttgcac caatatatac agctgtctca gccgcattag caaatccttt tgctattgaa
    15481 agatgtgttc ctaatttaat catattaatc tccttttatt tgtactacta cttgtactca
    15541 attttaattt tgaactactc caccttaact acatttagcc aacatatgaa gatggagatt
    15601 cctatttgat agtacttttt cactaaaaat tcgtaagctc taagggatgt tctatcccaa
    15661 ctaatctcat tcctctttta acatatttct agcattgatt taatttatat taatttttaa
    15721 ttttaatatt tacactttaa ttatacccat tttaatcaat ttcaaatatt ataatcttac
    15781 atactttgta tattatctaa gcacttctta gatagtgtat acatcttaca ctatacatat
    15841 tatttacaaa tacaataggt ataaaaaaac aaaaaaatag agattaaaaa tcattgtttt
    15901 acccatacca aaaactattt tttgattatt aagtaaattt tgatgttcaa tctctaatat
    15961 tatatcttta attaatccat aattaattct ctaactagga cattcctcct caagagtagg
    16021 aactataacc atagctatat catacttaga accttcaaca ttgtgttgtc ttttatttaa
    16081 aatttcatct atctttgact tcatagtgtc actaagttca actaattcat catgagtaag
    16141 agaaatatca tgataatcaa ctatattttc attttctaaa acatcttcct ctgctgcctt
    16201 ataaaaaact tcactcatat tttcaaattt tgatatgtac aattcttctt tttcttcccc
    16261 attgtctaaa gaaaaattaa gtatcctatt ccctataacc acattttttg cacttggata
    16321 atagtacttt tccacaatcc ccgactttat tttttcctca actaattcta aaataccaac
    16381 tttgtataac atttttatat ggtagtttat ttttgcatga ggttcttcca ataattgaga
    16441 taattgtttt gctgacaatg gcgatttgtc aaatgctttt agaatatcta ttctacgtgg
    16501 atgcgcaata gcctttatgc actccaaatc tcgtaatacc aatacctctt tcattgaccc
    16561 ctcctaaaaa atttatttaa ttcatatttc tttacaattt aattatatat taaattatat
    16621 gttgttgtca aaaaaatcct ttcttaatat tttaaaaaat atttaatttg agtttatttg
    16681 acaactcctc taaaacttta actcctgcga cgctgttacc ttttttgtca agcgcaggtc
    16741 cataaacacc aatccccatg cgtcccggca cagaagccat tataccaccc ccaactccag
    16801 attttgcagg aatgccaact ttaatagcaa attccccaga agcatcatac attccacaag
    16861 tcatcataaa tgtcttgaca atcctagaaa ccatttcact cattaaatgc tcaccatttt
    16921 ctatatctac tccataattt gcaagattta ttccaattct cgctaaatct acagaatcta
    16981 tctcaattga gcattgttta aaataaaggt ctagaacatc ttctacattc cctctgataa
    17041 atccatcact tttcaataag taagccatag ctctgtttct atctccagtc attttttcag
    17101 acttatagac atcatagttt attcctatat tatcattttt tgctaacttt ctaaagaaac
    17161 taagcattct ttcttctttt tcttctaaac aactcccatt tataagggaa gtggttacta
    17221 tagcccctgc atttatcatt ggattacagg gtttttttgt gtcattaatt tcaagtttca
    17281 ttattgaatt aaatgggtcc ccacttggct ccattccaac gtttgaaaaa acgtcttccc
    17341 aatcattatc cataagtgcc atagcaagca caattggttt tgaaatactt tgtatagtaa
    17401 actttgtgct acagtttcca gcactataaa gattattttc gctatctatg atacaaattc
    17461 ctaaatcatt tcttctagcg ttttttaatt ctggtatgta actagcgact tgtccataat
    17521 tagtatactt cttattagaa cttataattt cttttaataa tgtctcatct aacgtattca
    17581 tatgcacctc ttatattatg ttatatattc tattttagca caagtttata ctataataaa
    17641 acaatgttaa aaaaaattat actttaaaat tattttctac aagaatcaat tcatttttca
    17701 aacaatttaa atagtttaaa tagcttaata atttaataat ttaacaacct agcaatttag
    17761 taatctaata atccagtaat ttaacaacct aataatttag caatctaata atctaactat
    17821 ctaataatcc agtaacttaa caacctagta atttagtaat tcaacaatct aacaattcaa
    17881 taatttaaca atttaacaac ctagtaatct aactatctaa taatccagta acttaacaac
    17941 ctagtaattt agtaattcaa caatctaata attcaataat ttaacaacct agtaatctaa
    18001 ctatctaaat aatccagtaa cttaacaacc tagtaattta gtaattcaac aacctaataa
    18061 ttcaataatt taacaaccta gtaatctaac tatctaaata atccagtaac ttaacaacct
    18121 agtaatttag taattcaaca acctaataat tcaataattt aacaacctag taatttagca
    18181 atccagcaat ctaataatcc agtaatttaa atttttataa caccaaaaat ttctaaccca
    18241 acacctttta ctcttttact cttttactct tttaaaaaat aatcacataa attaacaaaa
    18301 tattaaacaa aaaagtaaaa tttaaaataa aaagcttgct tcatacaaaa gtataaaaca
    18361 agccttttta tatatcaact caaactttaa aatctgtatc aatttaattc tccactttac
    18421 acttaatata cttaacataa accaataaca ctacttgcta tgccatttaa tcttcataaa
    18481 acaatctata atacacacat taatcaacta ttttacttcc tccaataaat tccctaagaa
    18541 ttgaaatacc tggtatgtca gaagtatcgt ccaattcaac aaaatactgc aaaaacttta
    18601 gtaatctagt agcttcaata tgtgcaggat tatcttcctt tatctcttcc aacaaaggtt
    18661 tcgcaaatcg ttttaaaact gccaattcat atacacaagc agagttaaat atagcatctg
    18721 cctcctcttg gtacgggaat atattcttct tttctcctct tctaactgat gcccattgta
    18781 aaatagtatt atcagcacta tatcctctaa aattatagtc tctaacaatt cttcttatca
    18841 ttctcaaatc agcagtaggg attcgattat ggtcatccaa atttatttga gtaagggcac
    18901 ttatgtatat cttaaattta tcttcatctg gtatagatga tgttaaaata ggatttagcc
    18961 catgtatacc ttctaatata attggctgat ttgaatctat ctttatcttt ttaccagtgt
    19021 attctctctt accttcttta aaattgaatc ttatatcatc aatttcttca ccagctaaaa
    19081 gtttctttaa gtctaagtta aacttctcta aatctattgc ataaattgat tcaaaatcat
    19141 aatttccata ttcatctaaa ggagtatgtt ttctatctat aaaataatca tctaaagaaa
    19201 ttgaaatagg atttaaatta tttactctaa gatgtattga taatctgtga gcaaaacttg
    19261 tttttccaga tgaagatgga gctgctatta aaactactct cttattgcca tcctttatca
    19321 tatccgctat ttgggataac ttcttttcat gcaatgcttc aaatgtcctt attatatctc
    19381 catattcatt gttttctatt attttattaa gagatactac cttgtcaatt cccataagtc
    19441 ttgaccattc ttctgcttca taatatacac ttgatagctt aggttgaggt acaaatttca
    19501 taggaagagt cttatcttct tcactaggtc ctaaaattat aacgcctcca ttataaagct
    19561 taatatcaaa tgattttata taaccagttg atggcaacat ataaccataa aaatggtcta
    19621 tatagtttct acacttatat actttagcac tatcatactc tttgtatttc aaaagctctg
    19681 ccttttcata catttcatta ttttcaaaga tagaaatagc ttcatttctt gtaacttcaa
    19741 ttttttctat aacataatcg ttgtttacta tctctttcat cttgtttttt atattttcaa
    19801 catctgcttc tttaagcttt ctatctatat gaacttcaca atatagccca tctgatagtg
    19861 aatgttctat tgtaactctg ctatcccaaa agatttctct acatgccatt acaaatataa
    19921 atgacatgac tctaaagtaa actctctctc catcttcatt agtagtatct aaaaaattta
    19981 tctcacaatc cttcttaact ctacaattta gctcttttag tttattgtct acaactgcaa
    20041 gtgttatata acccttatgc tcatcacaaa attcttttgc tatatctccc aatctgattc
    20101 caggggaatt ttcttctaat gaatattcct tatcttttcc ttctattttt aagtttattt
    20161 ttttcacttc aacatctcct tccttttgat gaatataaat tttaattaat gaggcatatc
    20221 aacaagataa agagaggtat ttaaccctct cctacaatat tattcaattt acttatagta
    20281 tcttcaatgt cttcgtaatt tatttctaat actaaatatt ctgcatctaa tgttaagatc
    20341 tctttaatcc cctcttcaga taaaattcct cttcctatac caacatgtgt atcattgata
    20401 ccatcattat cacttaagtg tatcagtctt attttatcct ttaagttctt tacataagca
    20461 tcctcatcaa ttaaacagtg tccagtatcg taacaaaaac aaatcttaga atttttaatc
    20521 ctcttaaata tatattcaaa atcataagat atatttccaa tatttgaaaa atccccacct
    20581 tttgaataag tattctctat acatacatat gaatttgtat ttaatttatc taaaaaatcc
    20641 acactatttc ctaaatattt gtctctattc tttctaactc tatttgtcat aacatatccc
    20701 aaatgaaggt taaaatacct taaatcaaat ccttttagtt caaattctat cttctcaata
    20761 aaatttatcc atgaatttcg tatatactct atgttttcgc aagtatttaa ttccataggc
    20821 aaatgtatcc ctatagataa tcctaactta gaaattctct ctttatattt acataactca
    20881 ctacattcag atatattgtc aattccaatc tctaaatgag ttatttgctt tatactctca
    20941 caaatgttta aagcctcctc aatattaaac aataaggacg ctataccaac tgccatacaa
    21001 atcacctact caaaaatacc tatatctgta aaaggaaata ctcttaaaat aacttttcct
    21061 ttaatatttt cttcatctac taatcccact tctttatatc tactgtctaa actaacttct
    21121 ctattgtctc ccatcgcaaa tacttttccc ttaggaatca ccatatcaat atcgccttca
    21181 gtataattat catgtatata tgaaacttca tttaataact taccatttat ataaacctta
    21241 gaatccttaa tttttagatg gtcaccagga acacctataa ccctttttac aagctctttt
    21301 ttccttccat tctcttgtaa taaatcagtc ttaaatatca ttatatctcc acattttggt
    21361 tctgataact tatatgtcat cctgtttgct accaaataat catgtgattt aagtgttggg
    21421 tacattgatt ctccatttac taatgttggt tttataaaac gagttataat aaatgcaaaa
    21481 aaaagtgcag taataattat ttttatccac tctacaatct cttctttaat agtttcattc
    21541 attcacaaat tctcctttat agttatatta tctttattga taatgtctct taaacactat
    21601 cttttagata agtctactaa ataaggtgtt gtctttttta tatagacaac tattgtgtat
    21661 ggtctggtga tttcagctaa tcagcgattg ctccaaattt tatatgttta gtcaatagtt
    21721 cctatcttag aaaatggata caatctgact agaacctttc ctaaaatggt atgctcgtcg
    21781 accaatccaa cctcatcgta tctactatct aaactttttt ctctattatc tcccattgca
    21841 aataattttc cttctggaac tacgatatca atatctccat cagtacgatt attatgtata
    21901 taggaagtct catctaacaa cttatcattt acatatacct tggagtcttg tatttttaca
    21961 tggtcaccag gaaccccgat aactcttttt actaaatctt ttttctttcc attttcctct
    22021 gttaagtcgg ttttgaatac tattatatct ttgtattttg gctctcctac cttgtacgca
    22081 attctgttaa ctatcaaata atcacgttca actaatgtag gatacattga ttctcctttg
    22141 actattgttg gcactataaa acgagttatt gcaaatgcca aaacaagagc tatgacaatc
    22201 acttttatcc attctacaac ttctttttta actgcttcac ccactaaaat cgtctcctct
    22261 gtgttaaata ttaagtcaat ctacagcaaa ataatctacc ataaacctac aatataaata
    22321 ttatacttct tttttctaat agtttaaaat acttttctaa gacctttagg ataatgattc
    22381 ttaattacac cagaagattc ttttccccaa cctaaaggta ttccttcaac cgaaaccaaa
    22441 acccatcctc tactatcatt agaatttaca acatcccctt ttaaatattt aagaattgat
    22501 tcactctgta cactgaagtt ttcaacattt tttacatctt caacttttaa gtaatgagat
    22561 agtgcatgag aaggctcaaa tctattcttc tttaaaaccc ctaaatgcaa accatatctc
    22621 aatactttta gttttttcgt atctggatat atttcaggaa gtaggtacaa attttctcct
    22681 cttaaataaa atttattttt tttattacta taaaaatcaa cctttaaaaa tttcttttca
    22741 aactccctat aatcctttat ttctttatct aaatttttta tttttaattc tttgacatta
    22801 cattcttctt cttctaattt ttttattttg gcaacaaaat gaccttctcc ctttatctta
    22861 tgaggccata atctttccat ttcaataagt tctgcatgtg gatattcact gataaactca
    22921 tttataacat gctcattttc ctcccttgaa aaagtacaag ttgagtaaat taaaatgcca
    22981 tccttcttta acattttata tccatctctt ataatctctt tttggatgga ttgacattct
    23041 actacctttg cataactcca atctgatatg gcaacctcat ccttcctaaa cataccttgc
    23101 ccagaacaag gtgcatctat aataacctta tcaaaatacc cattaaacgt ctttcttaag
    23161 ctattagaat cggtgtttgt tataatacaa tttctcgctc caaatcgctc taaattttcc
    23221 cctagagcct taattctact agcattaatt tcattagaaa ccaataaacc tgtatcgttt
    23281 agcttagata gtatatatgt agattttcca ccaggagcag cacacatatc gagaactttt
    23341 tcaccctctt taatatctgc ctttggtatt acactcattg cagaaggctc ttgcaaatag
    23401 tatgctccag cctcatgcag tggatttcta ccaggtctgt caattttatc atcataataa
    23461 aaaccttctt ctgcccatgg tatttgattc aaatcaaaca actccaaatc aaataatttt
    23521 ttcttgtcaa tttttaaagt atttattcta agcccagtag tcttactttc ctcataactt
    23581 tttataaaat catcatattc atcttttagt atttcttcaa tatcttctaa aaattccttt
    23641 ggtaattctg tcaaatctat aatccccctt caatctgctt tacttgtgtg tcatttatta
    23701 tactttaatt atttatgtct ttcctttaaa ttttatttct tcttcttttt cctaagatta
    23761 ccgaaaaaca cttttttaga gtgattcata tatttctcaa gtttatcaag atttatcttc
    23821 cacaaaatta ttacataaac tataagtaat ataatagccc atctatattc tatcatcaat
    23881 atgaaatcaa aaactccatg taataaaatt ggaattaaca ccgccataaa caaatactca
    23941 cgtcttttat ttttatttcc ctcaaattta tacttagaaa tatagtagcc catagtaatt
    24001 gcaaacatta catgagctgg tatagagata actgccctat taataccaac ttgcagtgct
    24061 agatttctac tttcagaaaa aatataaacc atattttcaa ttgttgcaaa tcctaaagct
    24121 aaaaatacag agtaaataat accatctaac ttttcagtaa agtgtttttc tttaattagg
    24181 gcaggaatca gtatcagagc ttttacacct tcttctacaa gtcctgcaac tacaaatgat
    24241 acatatatta gttctgaata cccttcaaat acattaaact tcatcagtaa attttctaaa
    24301 agtataatta taacacttag aaaagcacct attaaaaaaa atttaattag atggattagg
    24361 ggttctctac aatatctatc tttagacctt atccaaaaca tccctatcaa tataggaatt
    24421 attgctaata aaaataaatc tagcttcata gattattttc ctttcaaaat ttttgattta
    24481 tttatgattt atatcatcta cttattttta acttattcgt gttttaaata catactaaaa
    24541 tatacattaa attattttaa attatttaaa tacaaatata ctcaaatact caaaaaaatt
    24601 taaatttata tctatattat ttaacaaatt tcaagctata ttcatatctt gaaatataag
    24661 ggaactaaat tcatttaaag aaagggtgat ttgatgcaag atggtttctt aacagtaagc
    24721 ataattgatg ctactaacaa tagacctatt caaaatgcag tagtaaacat atattcaatg
    24781 tctaatggta gccaaagctc atcaacttta taccaaaacc taagaagtaa tgaatctggt
    24841 caagtgactg gtttagtatt accagcacca gatgtagatt actctctaca accatctgat
    24901 gttagacctt atagccagta tatagtggaa gccatagcag atggatatga gacagttgtc
    24961 atagaaggaa cacagttgtt agctacaatt gaagcaagac aaggagttcc catgtctcct
    25021 agaacaagaa gtaagcgttc tttctcaaga caaagtgagt tgatttttga tataggagaa
    25081 catactctat atggtactta tcctcctaag attccagaaa gtaacttaaa accattacca
    25141 cctcctactg gtttcgttgt attagataac cccgtagtgc ctgaatttat agtagttcat
    25201 gatggtttgc cagaagattc atcagctcca aactattgga ttccatttaa agagtacatt
    25261 aaaaacatag cgtcatctga aatatattca acttggcctg aacagacaat ctatgcaaat
    25321 gtaatagcta taatatcatt tactttaaat agagtcttta ctgaatggta tagaaataaa
    25381 ggatataact ttacaataac ttcaacaaca gcatacgacc ataagtttat aaataataga
    25441 aacttatttg aaccaattaa cgtagttgta gatgctattt ttaatacttt tataaaaaga
    25501 cctcctacat caagacagcc actattagca caatattgtg atggacaaaa atcccaatgt
    25561 cctgaccaaa tgactcaatg gggcagtaaa gacttaggtg accaaggata tgactatgag
    25621 agtatactaa gatatttcta tggagatgag atagtatttg aaagagcacc tatagtaagt
    25681 ggtgtaccag tgtcattccc tggtactaca ctacaagttg gttcttctgg gcagtatgtt
    25741 agaaccatac aaaatcagtt aaatgctata tctaattctt atccagctgt accaaaagtt
    25801 atagaagatg gtatctatgg aacagataca gaaaacgctg ttaagatatt ccaaggaatc
    25861 tttggtttac ctcaaagcgg agtagtagat tttaaaacat ggtatgaaat atccagagtt
    25921 tatgtagcta caactagaat agcttcttta aatcctttaa tttaaagctt gatttagaac
    25981 ataaactcaa attattgaat aaaattatta atttaaaata aaagtggctg ttatgaacta
    26041 atatatacca taagtattac ttataagtat tgtagtctta acagccattt tttagttata
    26101 acttgttttt ttagaatctt aaaactttaa atgggggatt ttaagcttct taaaaacagt
    26161 gcttagcaac ttagatttct ttaaaatatt atatttattt ttaagtttaa taaaatgcta
    26221 ttctaacgaa aaacctactt tttatttaaa atctcactta ttatttaaaa cactatccat
    26281 cacttagaat attacttctt atataaatta ttatttatta taaatacaaa taatattaat
    26341 ttaaaaagtt attcctaatt taaataatac tactatcttc atcagtgaac ctaaactgca
    26401 actttagact tattagctaa agccaatatt agacctaaag ctatagatgc aaatgcagaa
    26461 gcaattactc tatactcagc agggaataaa aatacaattg tatgacatgg tatccagaaa
    26521 aatggtatag tttttaaaac actaaaacca aggtaagcat tccagtccac aatatttacc
    26581 acatctttaa gttttacctt aatccccttt ccaacctcat attttttatc tataaaacag
    26641 tccataacct tatggaataa aaatacagtt ggtgacatta gtatattcat agttgcactc
    26701 ttaaatagag catttgcaaa agctattccc tcaaatggaa gcataccatc cacttgtgct
    26761 tgacctacac ctgatgagta gattgacatt ccatatgtgg taactacacc tattattccc
    26821 caaactattg ctctataaaa tactcctact ggtattttcc aatccttata caaaattcta
    26881 acaccaagta aatctcccat tgtagctaat acagcaaact ttaaaaaacc tccaatatat
    26941 ggatgtgcac ctgttgcttc cataaatact tctctactac ttggtattgc taagacagca
    27001 ataatacaaa taagaactcc actccatata aaatcgcctt ttttcatatt tataaatact
    27061 atttaatata taaatagttc tcctttctat taaagattta tttaatttac aatctatcta
    27121 caattattta ttaatcactt attattagat attcattttc catatttaaa tttattctat
    27181 ccctttatct atttattttc ttactactct atccaccatt atctatatat ttattttacg
    27241 attactcttt caccctaacc ctttaactat ctattttttc ttactattct tatccacatc
    27301 atccatatat ttattttacg attactcttt cactctaacc ctttaactat ctattttttc
    27361 tgatattctt atccaccatc atccatatat ttattttacg attactcttt caccctaacc
    27421 ctttaactat ctattttttt ctggtattct tatctactat tatctgtata tttattttat
    27481 gattactctt ttaacctacc ccctatactt tatagtaata tgtttttaac ctttttcatt
    27541 attcccaata tgctttataa ctacaaatac tctcccccct taaaaataat aataaaatta
    27601 atcaataaat ttataatcat attattactc tatgtattaa gcaattacta tgccaataaa
    27661 ctttgatttt tttaaacaaa tcttaaaaat cctaaaatgc taattttctt taacttttaa
    27721 tgaatctaat agataacatt taattttcgt tatatttttc agaatatttc tattattatt
    27781 tttaagaaaa aaaaataatg ttaacatttc ttaacataca ttaaaaaatg ttaacattat
    27841 aaattgggtt taatactttt actttgactc gatttacttt tattctactt cttactttat
    27901 ttttaactta gactttattt ttttattact tatttgagtc ttatttattt tattctcact
    27961 ccatatgttc aattacattt gtctaaattc tttgatactt ttattacata attttaacaa
    28021 gcttaagtgt tatttgggta gtgcagttga taattattta aataatcatc aactgcatgt
    28081 ttatatcatt acagtagctt aatgtattta atcatactta cttaggattt tgaaatatct
    28141 tagagattat atttaacatt agtcaattgc tgcataattg taagcaattg aaatttgaga
    28201 cccatcttca ttttctatag tgataggctt cataattttt aatccttcag tttttatctt
    28261 ttctaattct tgttccattt tcttaattgt ttcctcaagg tctttttgac ttacatcttt
    28321 gactgcatta gggtctttag catagtcttt taattcttga atatgtttag catactcttc
    28381 ataagtataa tgctcaacac catacttttc caattcagta gggtcagtta ttcctgattt
    28441 tattagagat tcaaatttag gtaaagcaat attagatgaa tcatttccat ttgcaaatac
    28501 tgctccagtt cctcctagta ccattgcaga taccatcatt gcacttataa gggatttttt
    28561 tattttcata accaatcttc ctttcttaga gatttatttg ggtagtgtag tttaaaatga
    28621 ctattgcaat agttatcaac tacactctta gactatcaca gtagtcatgt ttatgtcaag
    28681 cctttttaaa aaacttttca ataaaaaata ctatctcaaa gcaaaattaa ttattttgag
    28741 atagtatttt aattctaaat cttttatatt actaagattt atggttattc tatccatgtt
    28801 ctatattgtt ctttattaac tgctaacaaa ttcccttttc ttaatatgac aaagcgatta
    28861 tcattatagc cacttcgctc aattgaaact tctcctgaat cagtataaag tacatcctta
    28921 ctattactag atttatttac atacgtttct tcactattct cagaattagt tactttgctc
    28981 atagatgatt cttcatgctc tctaaattca attacagata cttttgccgt actaaaatga
    29041 gtctctccat cacttttagc tttactaaca agaatatcag attctacttt gggagaatat
    29101 aaatcagttg atttactact tattgaaaat ccaattttat cactttgttc attttttgtt
    29161 aaaccaaaag attcaacctc attaatctct ttattttctg ttgcaaaaac aagacctgtc
    29221 ccacaaacta atacagttga aactaaaaaa gtaacataag ttgatttttt aaatttcata
    29281 atattaacta ttctcttttc tatttttgtc tttccaaatc cactacatat tgtcattgaa
    29341 aatggattat tctcctctgc tagacttatt agtgtggttg cataatcagc cctatattct
    29401 tcaccaagta tactcataac cttttcatca caactaaatt ctatgtcttt atttagtaaa
    29461 aaaaacatca cccaaactaa gggattaaac caatggacaa caagagctac aagcataaat
    29521 aactttaaca gattatcaaa gctattaata tgaaccattt catgagttaa aacaaagtct
    29581 aaagtttgat tattagcata attcatttgc tttggcaaaa taattcgtgg ttttaaaata
    29641 ccagatgtaa gtggtgtcct agtaaattca gatatgcaaa caataaatct tctaaaacgg
    29701 ctattcttac acatccattt tttaatgaac tcatgatttt ttatatgaat tgaagtttta
    29761 aaaagattaa ttattttaaa atatctaaag cataaaagta tgctaaacat aatagtaact
    29821 ataaaagaca aaatgcccaa catttccaac atcataatat tttcaatatt agacattatt
    29881 tcaaattcgt cagaattgtt tttacctgta gagtcactag ctttcttaag agtataattt
    29941 tctgttgatg tattacccaa ttccacatca aaagtcgatt caattatact ataaatacta
    30001 aaaccagaat ttaaacttac tggtattaga agtttaaata aaacaatcaa ccacaataat
    30061 acaaatacct ttttaggtaa ttttttaaat aaacatgctc ttataaaaat aactattaaa
    30121 agtaatactc cagaagtaaa actcatttct aacaatttaa tgaacataaa tcttacctca
    30181 ctttaatttt tcaacaattt gtttaagttt tgtaatctca tcacttgaaa gattttcttt
    30241 attaataaaa gctgaaagaa atttttctga agaccctcca aacattttat taattaaagt
    30301 ttctgtttcc tgtttttgta cttcctcttt gcttattaaa gctttacaaa caaaattcgg
    30361 ctcaattctc tctattgcac cttttgacac taattttttt ataacggtat acgtagtgtt
    30421 tctattccat cctgtttctt cttttaaaat ttttactaac tctccagcct tgctttctcc
    30481 tctctcccat agaacttcca ttactttaag ttctgaatca aataatttta tctccatata
    30541 aaatcgctcc tcaaatatag ttatcacaat agtcattact ataataaaaa actatcatag
    30601 tagtctatac tatgtcaatc atttattttt taataataaa aactgtcttt ttaaaaaatt
    30661 cttataaaag acagttttat caatgttata ctttaatgtt taattaaagc catattttat
    30721 ttaaatatat aactatctat cttagtacat agtataagat ttttctttat ctaatagtat
    30781 atcctctaca tctgggtttt cttttactaa ttgtctataa aaattacaat tagtaacacc
    30841 cacatatgga attagccata gtagacctat tccaagagta gctattgaaa gtatatacca
    30901 tcctataaag ctaaagttta gtacaaataa tttaagtaca tttcctttca tcatatctaa
    30961 actagcaccc attgatttaa atacacccat attatccttg tcttcacaaa ttaagaaagt
    31021 agcttgagaa acatataata gcaatatata aactggtaac ataagaacaa gtattataag
    31081 aactagagtt attccagtag aaataatagt ccctattcca acattatcac ttgataaata
    31141 ttcaattgct ccactagtaa gtaaaactcc aaatgataaa aacgctccta taattgtgat
    31201 tataaaagaa taaatataca caataattga ttcaaataaa taaaaaccaa aacttcttaa
    31261 ataaattctc ccacttacaa agatatctcc tatcttgact ttttcatctt tagtaatttt
    31321 caagaacata catgtaaatc ctgctgaaac gcaaaaagct attacagcca ctacaatagt
    31381 agtcaatggt tcaaaaggta atcctacaat tgatattaat actatcaatg caatatatac
    31441 tgccatagaa attgcacata tcccccagtt tccagataac tgagctttag atactcgttt
    31501 taattcttgc cttgatacca taaaaaacac ccctttcatt ttataaaaaa acttttaaaa
    31561 atataaataa tcttttaacg tataatatta atatatcata ttttcgacta tataagatac
    31621 aattattcac catgttttat caagatatgc cataattgta ttactagttt tatatagtca
    31681 acattaccaa atacatcaaa taaatttgat taattattca acaagtaata a
//
LOCUS       DAMJYR010000036        31025 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.036, whole genome shotgun sequence.
ACCESSION   DAMJYR010000036 DAMJYR010000000
VERSION     DAMJYR010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 31025)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31025)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31025
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.036"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            358..2592
                     /locus_tag="PC471_001649"
     CDS             358..2592
                     /locus_tag="PC471_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HDF2788781.1"
                     /translation="MQNHNYIKMLNKKVEVLSKELDSRNFKLKTLNEIDFFSKIPNRE
                     FMYQHIDDCISKNIHIGIMLLNIDNFKLFNDIYGYLVGENLLITLTQNLLHFIPESKG
                     IVAYLKGEGFLIVLPNVSNKELNNMGATIIESARELKIRLPNEKEIYSNVTFSIGSTI
                     WNGEPNMTTLMLFNQVYSALKKAKSEGKNRYILFDFKDNSFYNKLENDKEFLSEISHV
                     LQTKQIEVFYQPLYNIKNNTIVGCEALARWRHPIKGLLTPDKFIPLFEQFGLITELDL
                     HLFEENCKNIRRWIDNNDVFVPICCNFSRLHFFNPNFPSMLKGITEKYSVSTSNFSIE
                     ITENILIENTNTIIQQLAELRSYGFSVSMDDFGSGYSSFGMLQNLPFDIIKIDRIFLS
                     HNLNDFKNTTILYSIINIARALGMLTVCEGVETEQHVEFIKSINCDIAQGYYYAKPME
                     CKEFEKLLSKENNSEKMFIKYSDIGKSLIEKIFDGFFIMQDMKKLRANVTEDIQWFDF
                     YNKTEITSFPKVIEHFEKHIKGKKFSILYKSITPHNTESDSLLISGEAVLSDEASEHQ
                     KYSNFYFSANCIISENKLLLAKLHLDLIKTAGYINEFESYSIPKMTNTHLDESNILSQ
                     FYSMLPVGIIRYDLFGDMIITYMNEEMFDIIGYTKEQFFGEMGGNLRAIVHPDDLDFV
                     YKKSLDMIDTNKIEPFLYRFIKRDGQIVTVMYTQCNLSAIDGRSIAQGMYVDVNKIKN
                     LTKI"
     gene            2623..3468
                     /locus_tag="PC471_001650"
     CDS             2623..3468
                     /locus_tag="PC471_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HDF2788782.1"
                     /translation="MFKEIFLRTFPGFLIIRDSNYRIIFINDNLKNLIKSYMQDNPLG
                     MTNIEIAKKLPDNIAKFFTDSHNIQLDWEKNYPYDKISNWILEFKKDTTSYWNVLEYK
                     VDVDEKTYIITMANDITKLYEENKRNLHYSITDPLTGAYNRKYLNDRFDIFIGDYIVL
                     IDLDNFKMINDYEGHNVGDKILCDFVSLLNKELINSTSIIRLGGDEFIVIFSSDVDKN
                     YVYSQLEALRENFLKVFSKYKYLSFSYGVDTVKRNLKLTIVELDKKMYKNKEKNKKKF
                     DKNDY"
     gene            3708..5441
                     /locus_tag="PC471_001651"
     CDS             3708..5441
                     /locus_tag="PC471_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HDF2788783.1"
                     /translation="MSILLKKAPKLAKHIITSFYINRDINEVLKYLCENVTWIGPGEQ
                     EFLTSFNEIKNYFYAGQDEIPSCDINNDIFETVSEDENRCMVLGRYTVRTKENAQMIL
                     EVNQRCTFEIIEDRGKLLIKHMHISNPYGEMQLDEYFPTKIGTQSYDYLQRLLKEKTE
                     VIEMITNNINGGLKGSNDDSTYSFFYVNEGLPKILGYTYDEFMEMSGGSAVGAVYPPD
                     LPKALEDCERCFAKGPTYSSEYRIRKKDGTLMWVLDSGMKSLNSDGIVKINSIITDIT
                     QLKNIESELKLERERYRIALQNITDIMFEYDIENDTLIKYQRVEIDKKIELENFETKN
                     YSKLLESGKIIHLDDIGKLLEVLHGNLRETIEIRQVNSLTKNEWRWIRVQCSVIYDSD
                     HNPIKTIGVLKDITEDKSKLESLINQAQRDPLTQLYNQRVSQNLIEEYLCSSDSKNND
                     ALLVIDIDDFKTVNDTFGHLEGNEVLVAVSKILLHNTHDKDIVARIGGDEFTIFIKSL
                     TKDLIIKITNDILNDASKIKVKDNHKITLSIGIAFTDDSTKLYKDLFSKADKALYLSK
                     ADGKNCYSTYE"
     repeat_region   5773..6253
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=5839..5861
                     /rpt_unit_seq="ataccacttagttaatataaaac"
     gene            complement(6783..6887)
                     /locus_tag="PC471_001652"
     CDS             complement(6783..6887)
                     /locus_tag="PC471_001652"
                     /inference="COORDINATES: protein motif:HMM:NF024729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HDF2788784.1"
                     /translation="MDDYIYYYNNFRYQNRLNSMSPFEFRKYLSTERA"
     gene            7512..7757
                     /locus_tag="PC471_001653"
     CDS             7512..7757
                     /locus_tag="PC471_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2788785.1"
                     /translation="MNQKKIKISIKMVIIGIFSVIIAFVLSRLFVEYIDELLRSNWQY
                     QLFESESSYIIFANCIMFSNILLEIFLIYICRKFRKI"
     gene            complement(8510..8656)
                     /locus_tag="PC471_001654"
     CDS             complement(8510..8656)
                     /locus_tag="PC471_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2788786.1"
                     /translation="MVFETFDKTIELYPNAKPIFHSDIGFRLDDESFEEYLGRVFRKR
                     CKGI"
     gene            8689..9075
                     /locus_tag="PC471_001655"
     CDS             8689..9075
                     /locus_tag="PC471_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2788787.1"
                     /translation="MNNNKFVAPNNVTLVERCYRYIKDRECSFSPNTIRNRKNMIKNQ
                     IEVFFKDMKLIDITPSILQSYINNIYNEHMLNSIKNQVDFIKSVLKESYRLKEISENI
                     CDFVTTPIKKVVQLLSFIQNKKLNYY"
     gene            9942..11297
                     /locus_tag="PC471_001656"
     CDS             9942..11297
                     /locus_tag="PC471_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HDF2788788.1"
                     /translation="MDTKISINENMSLGKRFFKYLAPSVVAMWVFSLYTMVDGIFVSK
                     GVGELALAAVNISMPFINFIFAVSLLFSTGASTIIAIYLGKKDIKSANEVFSFNLVSI
                     IILSIIILAITFFNLDRLALFLGATESTIGMVKDYLGIIIFFNGFFIVSYSLEVIIKT
                     DGFPILATVGVIISALTNIILDYLFVIEFGWGVKGAGIATGLSQVFSTIFFLIHFLRK
                     NSTLNFSKFRIDFKTLRKIVFIGFPDSTTELSCGIVVLLFNLSLTKYIGENALIYYSV
                     INYINTLVLMTMMGITQGMQPLTSFYYGAGNIDNVKKLLKMGIKATIIASVAVFAICM
                     AFSGPIVSLFIHPEETMLFNEGVRVFKIFSISFLLVGINVIISGFFVSVEKPSISTVI
                     SLGRGLVIVVLSLISMILIFGGQGIWMTTIVSEFICLILSLVFLKKNFSTLDSNLNKV
                     A"
     gene            complement(11415..12083)
                     /locus_tag="PC471_001657"
     CDS             complement(11415..12083)
                     /locus_tag="PC471_001657"
                     /EC_number="6.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphthine--ammonia ligase"
                     /protein_id="HDF2788789.1"
                     /translation="MKEVLKLADEKFVMSFSGGKDSILALNRMLKKGYKPVALLTTIS
                     EEHGKSWTHNLEYNMLKQVSSNIGLPLLVAECGVEGYEESFERALIKAKNMGATICAY
                     GDIDIESHRKWDTDRCEAVGMKVDLPLWQESREELVYEFIDSGFCSVVTKVNLKHLGE
                     EFLGKKLTRELVEKIKNAGADPCGEHGEYHTFVVDGPIFKKPVEYEVKGTLIKDGYGY
                     LEIK"
     gene            complement(12150..12578)
                     /locus_tag="PC471_001658"
     CDS             complement(12150..12578)
                     /locus_tag="PC471_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HDF2788790.1"
                     /translation="MTKLWAIIFALLAGGSTALEAFINGELGKSTTALVATFISLTVG
                     AIFFLASIIVTGDLKCLVSINGFNYKLLLGGVFGGLIIYFTVKAIPNLGVSNTLILTL
                     VSQVLVGFFIDSVILGQETMHLYKYIGVILLILGTFFIVN"
     gene            12685..13278
                     /locus_tag="PC471_001659"
     CDS             12685..13278
                     /locus_tag="PC471_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HDF2788791.1"
                     /translation="MNFCYNCSIDFGGDFMYTNFNIDFNNFNKKENAIKFMLMGVLLI
                     ILGLLCLTFKTLGIKLISWTFGIALLFFAYLNLKNINELKRYATKEEIKPSINIQWVL
                     IIACILLFVFPQKIQSIFSLFLGFYLIFNQLVALVNSKNNPYSKFTTWNIVKILFGIC
                     LILSPLFLSRFIVSIMSFFIILFGLVLFFSGNTARKY"
     gene            13397..14332
                     /locus_tag="PC471_001660"
     CDS             13397..14332
                     /locus_tag="PC471_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893027.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HDF2788792.1"
                     /translation="MDKFKYAFDNKRYHTWNYYLRNTFGEKVFKVSINAGFTCPNIDG
                     SLGYGGCTYCSKEGSGDFAGNPKDNLISQFYNIKEMMLKKWPHAKYIGYFQAYTNTYA
                     PLEVLKEKYETILELEDVIGLSISTRPDCLPDDVVEYLSELNKRTNLWVELGLQTIHD
                     STSKIINRGHDYKTFVEGVEKLKSKNIKVVVHIINGLPGEDYNMMMETAKAVGKMGVD
                     GIKIHLLHVIKDTPMEKMLQNGMLTLMEQDEYINLVCDQLEILPETMIIHRLTGDGKR
                     DELVGPIWSLKKWEILNKIDDTLKARNSYQGCKFV"
     gene            complement(14603..15547)
                     /locus_tag="PC471_001661"
     CDS             complement(14603..15547)
                     /locus_tag="PC471_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HDF2788793.1"
                     /translation="MGEKSKGYVFIAIAGLLWATLGLFGKFLMGNGLTSEQVAFTRLF
                     FGFIVLGVYSSIRTPQILKINKKGIIYSVIIGIICQAMFNLCYFKAIDIAGVSIAAVL
                     LYTSPLFLAIFSKLCYKENITRSKLFSLILCFIGAIMAVTGGRLDFQGLNAFGLLLGV
                     LSAIAYALMPTISKNALKEFSSSTILVYSFLFGAIFMIPSSRPWEILNYAKDLDVLSC
                     MLMLGIVPAALAYIFYAAGISKGVELSVAGVVASVELVGSVIIGCTILGESFSLGKLF
                     GVMLMLISAVVALNLSYDEIRIFYKSNKLKQIEKTESI"
     gene            15865..17295
                     /locus_tag="PC471_001662"
     CDS             15865..17295
                     /locus_tag="PC471_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDF2788794.1"
                     /translation="MNLDLEFYQKILEASHDEICVSDDKGIIIYCNKAFEENYGLKKE
                     DILGKNVSFLEDSGYSTKSPIPIVLKTKTKFSLEQDTQTGKKLIITATPIFDENGHLE
                     FTVENCRDITELNNIKNKLEDTKKQVKKYKSEVETLYRTALRIEDTVIMDGIVMRPII
                     NTVNHVSKTDVSVLLLGESGTGKSSLARYIHHNSNRSNGPFITINCATISPQLLESEL
                     FGYTSGAFTGASTKGKVGLVELANGGTLFLDEIGDIPQNLQAKFLQLIQDRTFTPVGS
                     LKNKKVDIRIISATNVDLVSKVKEKKFREDLYYRLNVIEIKLPPLRERRDNLVEIIKY
                     YFNRYSSDFNLNKTISKEAMDAIANYRFPGNIRELQNIIQKILLTCTDNHITIDDLPN
                     ILTKNIHITNNGNKTHISQINKVIISDSKSTNYKNKNFDTLIKEYEKNIILDAYEKFG
                     SSYKVAKHLEISQSKANRLIRKYTNT"
     gene            17495..18079
                     /locus_tag="PC471_001663"
     CDS             17495..18079
                     /locus_tag="PC471_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc dependent phospholipase C family protein"
                     /protein_id="HDF2788795.1"
                     /translation="MLMNTHFIMAKSILDNIDENKTFFISEKNFIYGNIKPDAFSKYK
                     LKKHYLDESFNMIVSKINYLCSLNIDSLSKYFSVSRLSQELGVICHFLCDFFCVAHSE
                     RWEFKHSLNKHVTYERELGAFAKDVDLSKIKCGTINSSFEKFFTKLYSDYKKKTDYMN
                     DLKFSAYTCNSVINYILDSILSNTIKSYHIANCG"
     gene            complement(18183..18962)
                     /locus_tag="PC471_001664"
     CDS             complement(18183..18962)
                     /locus_tag="PC471_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDF2788796.1"
                     /translation="MIKHIFCDLDGTLYENGTITKEDIVAIEEIEKKGVQFNVATGRI
                     FKQAHNIIKDSLDMNGYYVCENGSFIYDKDYNVIFKRTIDDNLVKKVIDRFESSDAQL
                     YFKYKGDVIVGSDTTAFRHYSSDFIVDPDFEKRSSFDNLIGNIGVCSENLEELSRIEL
                     YLKSEFSEVLEIYFSGTYTLNIVPKSVSKRGSIEHVIKTLNVSPDEVATIGDSPNDIC
                     MLEGFKYSFAMSKAREDVKQSANYVVDSVKSAIDVIMEINS"
     gene            complement(19020..20276)
                     /locus_tag="PC471_001665"
     CDS             complement(19020..20276)
                     /locus_tag="PC471_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011591286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HDF2788797.1"
                     /translation="MKAEIISVGTEILLGDIVNTNSQFLAKELASLGIEVYHQSTVGD
                     NKQRLLECFDESLKRSDFVITTGGLGPTGDDMTKETAAEYFGQKLELHKPSLEVLESF
                     FVKTGKKMAENNMKQVYFPKDAIVLKNNNGTAPGAILKKDGKFIIVLPGPPREMKAMF
                     NESVKPYLQQFTNEMLVSKTLRLYGIGESNLELEILDIINEQTNPTVALYAKELEVTI
                     RITAKAENEREAFKLIKPVEEKIKSRVGKYVYTEGDISISEGETALEDAVSKLLVEKN
                     LTIAVAESCTGGLVSSSLINYPGISSVFLEGCVTYSNDSKMKRLGVKRETLEEFGAVS
                     EQTAIEMAEGIAKGLKANIGISTTGVAGPGGGTKEKPVGLVYTAIYINGKTIVKKNIF
                     NGDRRKIRLRATRDLLNELRIQLEKL"
     gene            20534..21433
                     /locus_tag="PC471_001666"
     CDS             20534..21433
                     /locus_tag="PC471_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HDF2788798.1"
                     /translation="MKIAVIMGGISSEREVSLNSGKEIYNNLDKNKYEVVKVIIDDKK
                     DIFTKIPEDIDFAILALHGKFGEDGCIQSILETMDIPYSGCGPLCSGMCMDKNITKKM
                     LRDSNLPTAPWVLVKSVDEIDYDEIDNIGYPVFIKPNSGGSSVATFFIHSKDEVEEAV
                     RKGLEVDEFVMIEKYIPGGEYTSFILNGEVFPTISIKSDSGFFDYEAKYSVEKGAKEE
                     VVYLDEELQKRVNEISETCWKIFNCKAYVRVDMIISEGIPYVLELNTLPGMTQTSLIP
                     RSAAARGIKYSELLDKLIEYSLN"
     gene            21457..22905
                     /gene="ddlR"
                     /locus_tag="PC471_001667"
     CDS             21457..22905
                     /gene="ddlR"
                     /locus_tag="PC471_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator DdlR"
                     /protein_id="HDF2788799.1"
                     /translation="MIFSNLKLNDNEPIYIQLKNYISDMISKGLIPDNSKLPSTRELS
                     QLLQVSRNSVVLTYEELKSEGLIYSISGKGTFVKSKNKSSNTTWSLNWDCLENTYSKK
                     ANELDIIKSEIPWSSDLISFKSISPDGDLFDMEELKKSFLNRISLEGHKLLNYGYAQG
                     YKPLIDYLLEYMTNKGADISNKDILITNGFTEGFDIIMSSFTQEKDYIICENPTHNTA
                     IKIMKSHNLNILGVDINEDGLDFNMLEDNLVKYKNKIKFAYITPSYHNPTGIVMTPEN
                     RYKFYNLMKKYNIAIIEDGFNEELLYNSSHIFPICSLDNMNNGVVYIGSFSKILFPGM
                     RIGWIFADKKVINKLESVKRCRNIHVSFLDQGILFDYLSNGGFEKYIKKIRKFYGDKF
                     NFAYKCIKKYIKTEYILGDGGLHIFIKLKNIDTRVLLEKCYEKNVIFMPGDLFFTDNS
                     GFDTLRLGFSRLSFEDIEIGIKIIGETINEMF"
     gene            22964..23770
                     /locus_tag="PC471_001668"
     CDS             22964..23770
                     /locus_tag="PC471_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HDF2788800.1"
                     /translation="MNLILGDNMSKKSKRRKINKLKLTIVITVLFLALLSIYEILFSN
                     PNKKGNIKSQETMQNKNNTTDDSKNNQSSTKKQEDNNIKPTFSYSSIPDNIKNKMIGK
                     SMPTDEPISFDSLSYLKLTYYGFDEKTHQGEMIVNSELAPEVVDIFKELYEKKYPIEK
                     IKLIDDYDAVDEKSMSDNNTSSFCYRTIAGTNVVSNHGKGRAIDINPLQNPQVSGNDV
                     TPKVSTVYADRSSTKFGMIKKGDDCYNAFVSRGWSWGGYWKNPDYQHFEK"
     gene            23796..25715
                     /locus_tag="PC471_001669"
     CDS             23796..25715
                     /locus_tag="PC471_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_071223118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HDF2788801.1"
                     /translation="MNLMTLENISKSYSEKKLLENISLGINEGEKIGFIGVNGTGKST
                     LLKIIAGVEESETGNITKTNGIRIEYLPQNPTYNENSTVLEQVFNGTSEELKLLGEYQ
                     EILEKLNSNFTEELNKKLLSVHEKIDALNLWDLESEAKSVLTKLGINDFNQKIAELSG
                     GQRKRVSLASALITPCEFLILDEPTNHLDNDTIDWLEEYLNSRKGSLLMITHDRYFLD
                     RVTNRIIELDKGRLFSYDGNYSIFLEKKMERLSLEASIEDKRQNLIRTELAWVRRGAQ
                     ARSTKQKARLQRFDELINRDSFKPDENLDISVASSRLGNKIIEIHNISKSFEENTVID
                     NLEYTLTRTDRIGIIGKNGMGKSTLINILNGEIEPDSGYISIGETVKIGCFSQDDSHM
                     NIDMRAIDYVKEASDYIETSDGTRITASQMCERFLFNGTMQYTLIGKLSGGERRRLHL
                     LKILMTAPNVLLLDEPTNDLDIETLKILEEYLDEFKGVVVTVSHDRYFLDRICNKIFA
                     YEGNGKIHIYTGNYSDYLIYREIQGIEFEESKRESVKKDDSTTPKKEKPKNDKKLKFS
                     YNEQREFENIDSDIEKLESKIATLDESTAKFSTDFTKLQEILDEKSKLEKELEYKYER
                     WEYLNNLADEIANNK"
     gene            complement(25957..26304)
                     /locus_tag="PC471_001670"
     CDS             complement(25957..26304)
                     /locus_tag="PC471_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqqD family protein"
                     /protein_id="HDF2788802.1"
                     /translation="MSKSNDVLDIVFKITDGLEYEVAENRVVTILEKQDHKIQKFFRK
                     LKARIPKYKKIELDEYSSFVFLQIDGQRTVREIGKNLEAKYGEESHPLYERLLVFLNH
                     IEVNCHYIERLDI"
     gene            complement(26291..28219)
                     /locus_tag="PC471_001671"
     CDS             complement(26291..28219)
                     /locus_tag="PC471_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide transporter, OPT family"
                     /protein_id="HDF2788803.1"
                     /translation="MNKKLPKGAYGEVSGKDYVPYITDKSRTGGNVAVLIIGIILAAI
                     FAASTTYSGMKAGLTVAAGIPGAIIGSAFVGAFARSKGILGKNLIQGMSSGGESVASG
                     FIFVLPAVILIGSQITFLEGLAVGVGGVLFGIGIAAIVHNYLIVEEHGKLMYPESMAI
                     SETLVASEAGGDSIKYMGIGFVISGFITVLTGSFLNVANNVMSLVGSKFYKWKFDIEV
                     NPLLLGIGFIVGLEVSLTMFAGSILSNFGIAPLIGYFTDMAKDGAMVWNNPAMPLNQM
                     DVGAISSSYVKYIGAGMMLCGGIIGAIKLIPTIIASIKETLKAKSSAGEGEEGSSIQM
                     ILLLGGVVIGFLAAFLISGNIVMAIIGAIISLLLSLLFVIVAGRLTGTIGTSNLPVSG
                     MTIASLVIVTLVFVIMGWTDLEANKSLLLFGSFIVVAIAIAGGYTQSQKVTYIIGGSK
                     NEMQRYFTIASIVGVIVVVGVILLLSDQLRATGDNVQFALPQANLMSTLTSGIMSGSL
                     PWVMIIVGVFMAIVLYALNLPIMTIAIGFYLPIATTSIILVGALIRLFVELVSKTEKE
                     KEVKVSNGISLSSGLVAGGSIIGLIGIILQVTGVVTPKVPSGFAATNSMAIALLVVLV
                     VLTALPIVLSKVKNNEQE"
     gene            complement(28631..30820)
                     /locus_tag="PC471_001672"
     CDS             complement(28631..30820)
                     /locus_tag="PC471_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HDF2788804.1"
                     /translation="MYEVNLERAMNYWMDLLDDYTNIAKIPNDFFYKDCSDEVVDEVF
                     KMNIEKHERLIQYSKVNNISIDSIIETIYGLILQKYIYEDDVVFGKTMNIIPIRVRVR
                     QSEKFIDVVRKLKRQWSRSLEYGYYLLSDIENKNHLNTKLINTIFIAKSFDLSYDYDI
                     EKKGYDLSVIVYTKRALKIKIKYKPSIYKRETIRRILNQFDYIIEQIIEDNDILIEDL
                     EFVCKEEEKVLLEDFNNNQYEVPYDKSYVDLFREQVKVTPSNIAARDENRKLTYKELD
                     EVTDKLAGYLNHIGVESEDIVAVMLPRDINIIITAIGIMKSGGAFFPIDTSNPEERLN
                     YLLEDSNAKVVITTDELKSKVVNENTIVLDINDEEMFKFGYELTEKITSSNCAYTIST
                     SGSTGRPKTIAIEHKSLVNMCYYSVKSISATENDICGIYLSFSFDAVMKQLFPYLLVG
                     ASIDIMPEEAKFDEYTVNEYCEENDITILALPTAFARLFIQNCNNNSLRVVQTGGERL
                     KGYRKRNYELYNEYGPTEFTVVSTSFHVDREYGKIPIGKPIFNTYAYVLDKKNKLCPI
                     GAPGELCLSGIQISRGYLNKKGLTEQVFVENPYKTCEHNKLMYRTGDLVRWLDDGNLD
                     YIGRMDNQVKIDEFRIELYEIENIINNITEIKSVVCISRTNDDGDIYICAYYVIDEED
                     SGKINERTIREYLNEHLPPYMIPTIIMRIDKIPVTPIGKVNKRALPE"
BASE COUNT        11655 a         4363 c         3944 g        11063 t
ORIGIN      
        1 aataaaataa ttatgccttt gaaaaatcat taaacttaaa attaattata aaaaaactct
       61 accttactaa ctatacagct cacaatctat ctatttatga aaaaatatga tataattgca
      121 ttcaaaaagg aaatctttca atatactaga atttaagcat taactaattt tatctaatat
      181 tattactaaa ctaaaaaata tttgtatttg tagcattgga gcaaataact taatgtatat
      241 tcattttaaa tacttattta aaatttagca tttaaattat ttacaaatta acatttaaat
      301 acttctagca taaatacagt tgtcatcaaa atttaaagta tcttatggag gaataccatg
      361 caaaatcata attatataaa aatgttaaat aaaaaggtag aagtattatc taaggaatta
      421 gatagtagaa attttaaatt aaaaacctta aatgaaatag attttttttc taaaatacca
      481 aacagagaat ttatgtacca acatattgat gactgtattt cgaaaaatat acatatagga
      541 atcatgttat taaatatcga taactttaaa ttatttaatg atatatatgg atacctagtc
      601 ggagaaaatt tattaattac tctaactcaa aacctattgc actttattcc agaatcaaag
      661 ggaatagtcg cttatttaaa aggagaaggt tttttaattg tacttccaaa tgtatcaaat
      721 aaagagttaa ataatatggg tgcaactatt atagagagcg cccgagaatt aaaaataaga
      781 cttcctaatg aaaaagaaat atactccaat gttacattta gtatcggcag cactatctgg
      841 aatggtgaac caaacatgac tactttgatg cttttcaacc aagtgtatag tgctttaaaa
      901 aaggctaaaa gtgaaggtaa aaatagatac attctatttg attttaaaga taatagtttt
      961 tacaataaat tagaaaatga taaggagttc ttatcagaaa tatcacatgt tctacaaact
     1021 aaacaaattg aagtatttta ccaaccactt tataatataa aaaataatac tatagttgga
     1081 tgtgaggcac ttgcaagatg gagacaccct attaaaggac ttcttactcc agataaattt
     1141 attcctttat ttgaacagtt tggtctgata acggaattag atttgcattt atttgaagag
     1201 aactgtaaaa atataagaag atggattgat aacaatgatg tttttgttcc aatttgttgt
     1261 aacttttctc gtcttcattt ttttaaccct aattttccaa gtatgctaaa aggtattact
     1321 gaaaagtaca gcgtctctac atcaaatttt agcattgaaa taacagaaaa tatattaata
     1381 gaaaatacaa atacaattat tcaacaacta gctgaacttc gttcttatgg tttttctgta
     1441 tcaatggacg attttggttc tggatattca tcttttggta tgttgcaaaa tctacctttt
     1501 gatataatca aaattgatag gatattttta tctcataatt taaatgattt taaaaataca
     1561 accattttgt actctataat aaacattgca agagctcttg ggatgcttac agtatgtgaa
     1621 ggtgttgaaa cagaacaaca tgtagaattt attaaaagta taaattgtga tatcgcccaa
     1681 ggttattatt atgcaaaacc catggagtgc aaagaatttg aaaaattact atctaaagag
     1741 aataattcag agaaaatgtt tataaaatat tctgatatag gaaaatcttt aatagagaag
     1801 atatttgatg gattttttat tatgcaagat atgaaaaaac taagagctaa tgtcactgaa
     1861 gacattcaat ggtttgattt ttacaacaaa actgaaatta catcttttcc taaagtaata
     1921 gagcattttg aaaaacatat taaaggtaaa aaattttcta ttctttacaa aagtataacc
     1981 ccacataata ctgaatcaga ttcattatta attagtggag aagccgtttt atcagatgaa
     2041 gcttctgaac atcaaaaata tagcaatttc tactttagtg caaattgtat aatttcagaa
     2101 aataaattac tcttagcaaa attacatttg gatttaatta aaactgctgg ctatataaat
     2161 gaatttgaat catattcaat acctaaaatg acaaatacac atcttgatga aagtaatatt
     2221 ttatcacaat tttattcaat gttgcctgta ggtataattc gctacgattt atttggtgat
     2281 atgattatta cttatatgaa tgaagaaatg tttgatatta taggttatac aaaagaacag
     2341 ttttttggtg agatgggcgg aaatttaaga gcaattgttc atccagatga tttagacttt
     2401 gtttataaaa aaagtttgga catgattgac acaaataaaa ttgaaccatt tttatataga
     2461 ttcataaaac gagatggtca aattgtcaca gttatgtata cacaatgtaa cctttctgca
     2521 attgatggaa ggtcgattgc ccagggtatg tatgtagatg taaataaaat aaaaaattta
     2581 acaaaaatct agtacaaagt attttatttt ggagaaatta atatgtttaa agaaattttt
     2641 ttaagaactt ttccaggatt tctaattata agagattcta actacagaat tatatttata
     2701 aatgataatt taaaaaattt aattaaaagt tatatgcaag ataatccact tggaatgact
     2761 aatatagaaa tagctaagaa actccctgat aatattgcta aattttttac ggattctcac
     2821 aatattcaat tagattggga aaaaaattat ccttatgata aaatttcaaa ttggatttta
     2881 gagtttaaaa aagatactac aagctattgg aatgttttag aatacaaagt agatgttgat
     2941 gaaaaaacct acataattac tatggccaat gatataacaa aactctatga agaaaataag
     3001 agaaatcttc actactcaat tactgaccca ttaactggag catataatag aaaatattta
     3061 aatgatagat ttgacatatt tataggagat tatattgtac taattgactt agataatttc
     3121 aaaatgataa atgactatga aggtcataat gtaggagaca agattttatg tgattttgtt
     3181 tcacttttaa ataaagaatt aataaattct acttctatta tacgattagg aggagatgaa
     3241 tttattgtta tattctctag tgatgttgac aaaaattatg tttattcaca gctagaagcc
     3301 ttaagagaaa attttttaaa agtattctct aagtataagt atctttcatt tagttatggt
     3361 gttgatacag taaaaagaaa tttaaaattg acaatagttg aattggataa aaaaatgtat
     3421 aaaaacaagg agaaaaacaa gaaaaaattt gataaaaatg attattaaca ttgttatttt
     3481 aaaataaatt aaaattatct ttcttttttt aaacttgtac attagtttat aaatatgtat
     3541 ctatacaaga tattgacatt aagtactgaa gcatatattg tttcagttct tttttgttaa
     3601 aattaactta tttaacaaag atatcatagt ttaacaaagc ttttaattta aatatattga
     3661 tgtataatat aattaaaata taatagcaaa aagaaaggag gaatatcatg agcatactcc
     3721 taaaaaaagc acccaaatta gcaaaacata ttataacaag tttttatata aatcgagata
     3781 tcaatgaagt attaaaatac ctttgtgaaa atgttacatg gattggacca ggagaacagg
     3841 aatttctcac ttcttttaat gaaataaaaa attattttta tgcaggtcaa gatgaaattc
     3901 cttcttgtga tataaataat gatatatttg aaactgtaag tgaagatgag aatagatgta
     3961 tggtattagg aagatatact gttcgaacta aagaaaatgc tcaaatgatt ttagaggtaa
     4021 atcaacgttg tacatttgag ataattgagg atagaggaaa attattaata aagcacatgc
     4081 acatatcaaa cccttatggt gaaatgcaac ttgatgaata ctttcccact aaaattggaa
     4141 ctcaaagtta tgattatttg caaagattac taaaagaaaa aacagaagtt attgaaatga
     4201 ttacaaataa tatcaatggt ggcttaaaag gtagcaatga tgatagtaca tactcatttt
     4261 tctatgtcaa tgagggattg cccaaaatat taggatacac atatgatgag ttcatggaaa
     4321 tgagtggtgg ttcagcagtg ggtgctgtat atccaccaga cttacctaaa gcactagaag
     4381 attgtgaacg atgttttgca aaaggaccta catattcatc tgaatataga atccgaaaaa
     4441 aagatggaac attaatgtgg gtattagatt ctggaatgaa atcgttaaat agtgatggca
     4501 tagtaaaaat taatagtatt attactgaca taacccaact aaaaaacata gaatctgaac
     4561 taaaattaga aagagagcga tatcgtattg ctttgcaaaa tattacagat atcatgtttg
     4621 agtatgatat tgaaaacgat acccttataa aatatcaacg tgtagaaatt gacaaaaaaa
     4681 ttgaattaga aaattttgaa acaaaaaatt actccaaact attagaatct ggtaaaatta
     4741 ttcaccttga tgatattgga aaactactag aagtacttca tggaaatctt cgtgaaacaa
     4801 ttgagataag acaagtaaat tcattaacta aaaatgaatg gagatggatt cgtgttcaat
     4861 gttcagttat atatgatagt gaccataacc ctatcaaaac aataggcgtt ttaaaagaca
     4921 taactgaaga taaatctaaa ttagaaagtt taattaatca ggctcaacgt gacccactaa
     4981 cacaattata taatcaaaga gtatcacaaa atcttattga agagtatcta tgtagttctg
     5041 attccaaaaa taatgatgct ctattagtta ttgatattga tgattttaaa actgtaaatg
     5101 atacttttgg gcatctagag ggaaatgaag ttttagttgc tgtatcaaaa atactattac
     5161 ataacacaca tgataaagac atagttgctc gaattggtgg agatgaattt acaatattta
     5221 taaaatcatt aacaaaggat ttaataataa aaattactaa tgatatttta aatgatgcga
     5281 gtaaaataaa agtaaaggac aatcataaaa taacattaag tattggtatt gccttcactg
     5341 atgattcaac taaattatat aaagatttat tttctaaagc tgataaagct ctttatctat
     5401 caaaagcaga tggcaaaaat tgttatagta catacgaata ataaagctat ttatacgacc
     5461 aagaatgaac ctttaaattt aatttcatag tctaacaatc aaagagttga agctaatctt
     5521 atagctagtc aactagttcc tagaaaaaat ttaaacaata tatttattga tactgctcat
     5581 ttttctaaag agcaaattta tataaaaaat taaactttta tatacataca gatactaatg
     5641 ataaacagtt atcattagta tcttctatct taaaacaaaa tgataattta tctatataaa
     5701 aaagtgcttt gttgacagag ttcacttttt tatataggta cagaatttaa gtataaatac
     5761 atgttttatt ctatacattt tagttaatgt aaaatttctt gattgtactc tctaactttt
     5821 caacattata ctatttatat accacttagt taatataaaa ctataaaagt gttgcaatta
     5881 ctgggtttta taaagttaat ttacatacca cttagttaat ataaaacttt agcaccttat
     5941 ttatctatag agctacagaa agtatttaca taccacttag ttaatataaa acatgtattt
     6001 atttgttctt gtactattgt tttactctat ttacatacca cttagttaat ataaaacaat
     6061 atcaaaacaa ataaaaattt ttaataaatc attatttaca taccacttag ctaatataaa
     6121 acattctggt actgttgcta tttatgttcc gtattcagaa tttacatacc acttagttaa
     6181 tataaaactt tggagttgtt acagattatt tgccttatct ctctatttac ataccactta
     6241 gttaatataa aacccccaaa taaactgggc attttcaata cttacacata tgcaactctc
     6301 ttaaatttgc agtgaaccat gagtagtgca aatgataaag cttatcatat accctcaaca
     6361 ccttatattt caagcgttaa gctctacttt gtaacaaata tcgctcactg caaaactatt
     6421 tacattttta ttatatcata aatatattat ttttgaatat ctataccaat ttgtggtata
     6481 ataaaagcaa gaagaactac aatctattta gacgtagagt gaagttcaaa tactaatgtt
     6541 tattcttttt gaacttgatt tttagttcaa aactaaagtc actcctgcct gtgtgaacta
     6601 tacccgctgt tttatacagt taaaacatgt aaaaatagtt ataaagtact agtgtaactc
     6661 attagtactc tttttattat attcatataa ttaatatcta tattttaaag cttactatct
     6721 aaaaaagagc acgtcaaata aaaccctaga tatttaattt ttttagagat acttaaaata
     6781 aattatgctc tctctgtact taaatatttc ctaaattcaa atggagacat agaatttaat
     6841 ctattttgat atctaaaatt attatagtag tatatataat catctattgc tttaactaat
     6901 tattcatatg tatcaaattt ccttaaatag tacatttcta cctttaaaat tccccaaaat
     6961 ccttccatag gagcattgtc aaggcaacga ccaactcttg tataatatat gtatatctaa
     7021 caccattaag aaatatatat tttataatat atatttcttc atttaatgca cttagaaaag
     7081 caaagaaaat acagattagt acacttttta gagaaaaata aaattaatac atctaaaata
     7141 ttaaaaaata ctttagataa tctaaagaca catctcaaag tttttaacta aattataaaa
     7201 ctaattacaa atgctatgta acgataataa ataaaaaatt tacttcgctt aagcgacgtg
     7261 tcgttgaaat atttcatgtt gccaactgat tcataagtcg ataagaaaag aacactaatt
     7321 atttagagtt cttttcttag ctacaagtgg attataatac ttttaaatct gtgagaaaat
     7381 catatataat aatgatatga acaagtaaag aaaatacaaa aatgacaaaa ttataaataa
     7441 aatattataa aatcataagg tcataattgt tatattgtag aaacaaagta aagagagagg
     7501 gtggagaata aatgaatcaa aaaaaaataa agattagtat taaaatggta attataggta
     7561 tattttctgt aattattgct tttgtattat ccagattatt cgttgagtat attgatgaat
     7621 tattaaggtc taattggcaa tatcagctat ttgaaagtga gagttcatat ataatatttg
     7681 ccaattgtat tatgttctcg aatatattat tagaaatatt tttaatatat atttgtagaa
     7741 aatttagaaa aatctaatat ggtcaacgta tttcgaatcg aatacttcat gattatttag
     7801 tctattgtgc tagttaaagt ttaattccca tataaagtaa attgatttta gtaaaacaaa
     7861 tcacccctaa gggttagttt atggataagt catccaaata ttaactcttt aatttttgct
     7921 atagattcat ctttgctcat aactttactt ataaaaaatg aaatattata agctagaact
     7981 tttattctaa ttctagtcat aaatcctagc atagagttac tttttttata ttataaataa
     8041 aactatacaa caaagataat atttagtaaa aataactttc attttaacta aacttaaaat
     8101 tcaattactc tctaaattga cgtttaggaa ttagttcaaa ataaaaaaga gtgtatatat
     8161 tgaaattact acatttcaat atatatactc tttttaaatt tctcagtttt ccatggaact
     8221 ttactttatt tatttctgta ttagttgtat tttttatgca tttttcttta aaatcatatt
     8281 ttctgattca actcttatat tataaaattt ctttactaaa ttattctctt tttctatatc
     8341 ttcatatata ttttttaatc gccttttacc taatctttta tctaacatta ctaatgattt
     8401 aactaatata ttatatgatt ttatcgcatc ttctattgag atatttaaac atatatttac
     8461 tgcttcataa aaatcaaatt cattaaaaaa tccattttta tttacaatct tatatccctt
     8521 tgcatctctt tctaaatact cttcctaaat actcttcaaa actttcatca tctaatctaa
     8581 atcctatatc actatgaaaa ataggttttg catttggata tagttctatt gttttatcaa
     8641 aagtttcgaa taccaagtta ttattattcg attttccaat agtccactat aaacaataat
     8701 aagtttgttg ctccaaataa tgtaacactt gtagaaagat gttacagata tataaaagat
     8761 agagaatgta gtttttctcc taatactatt agaaatagaa aaaatatgat taaaaatcaa
     8821 attgaagttt tctttaaaga tatgaaatta atagatataa caccttctat tttacagtct
     8881 tatataaata acatatacaa tgagcacatg ttaaattcaa taaaaaacca agtcgatttt
     8941 ataaaatcag tattgaaaga atcttataga ttaaaagaaa tatcagaaaa tatttgtgat
     9001 tttgttacaa ccccaatcaa aaaagtagtt caacttctaa gctttataca aaacaaaaag
     9061 ctcaattatt attagaaaaa tcaaaaaata ttcctatagg tataccaatt tttttgatgc
     9121 taactcttgg attacgtttt ggtgaagctg tatcacttat atggtcagat gtagaccttg
     9181 ataaaaaatc tcacatgtaa atcaaacatt agtttatgta tacaataaaa taatcttcaa
     9241 agaccctaaa acccctatat caaaaagaaa attatttgct cctgatgaac ttataagttt
     9301 gcttaaaggg gaaaaattaa aacaaaataa gttaaaatta cagggtatat tgaaaaatga
     9361 atttgatttg atatgcttaa ataaaagatt caacccgtga gttcaaccaa cttttgttgt
     9421 tcattttaaa aaactattaa gagataataa tcctagagat ataaaagtac atgaattaat
     9481 acatgcaaat gcaactttga tattactttc aggtactaac ataaaagcta tttataaaat
     9541 attagtacat gcagatataa aaatagctat gaatatatat tccatattct cacttattag
     9601 atgaaatgaa taaagaaaca tctgaaaata taagcaaaat actattcaaa taatgttcag
     9661 ttttatgtca attaaatgtc actcaaagtg cttttttttc atttgtcagt taaatgtcac
     9721 ttaaagtacc tttttcctat ttgtcagtta aatgtcagta atatataaat aagatatatt
     9781 tataagaccc ttaaaatcac tattatttaa taaaatttat aattatatac tatttgctgt
     9841 tgacattggt attataccat agtttatcat ttatattaga aataaaatta ttaattaata
     9901 gtttcgtaat tttataatag atttacaggg agtgatagta aatggataca aaaatatcta
     9961 taaacgaaaa catgagtcta ggtaaaagat tttttaaata cctagcacca tctgtagttg
    10021 caatgtgggt tttctcacta tatacaatgg ttgatggaat atttgtaagt aaaggagttg
    10081 gagaattagc tttagctgct gtcaatatat caatgccttt tattaatttt atatttgcag
    10141 tttctctatt attttcaact ggtgcatcta ccataattgc aatctatctg gggaaaaaag
    10201 atattaaatc tgctaacgaa gtcttttcat ttaacttagt atcaataatt atattatcaa
    10261 taattatatt agctataact ttttttaatt tagatagatt agcactattt ttaggagcta
    10321 cagaatctac aattggtatg gttaaagatt atttaggaat aataatattt tttaacggat
    10381 tctttatagt gtcttattct ttagaagtta taataaaaac tgatggcttt ccaatattag
    10441 ctactgttgg tgtaataata tctgctctta caaacatcat acttgattac ttgtttgtaa
    10501 ttgaatttgg atggggtgta aaaggtgctg gtattgcaac tggtttgtct caagtctttt
    10561 ctactatttt cttcttaata cattttttaa gaaaaaactc tactttgaat ttttcaaaat
    10621 ttagaattga ttttaagaca cttagaaaaa ttgtatttat aggttttcct gattcaacta
    10681 ctgaattaag ttgtggtata gttgttttac tttttaactt aagtcttact aaatatattg
    10741 gagaaaatgc tcttatttat tatagtgtca taaattatat aaatacatta gtacttatga
    10801 ctatgatggg tataactcaa ggtatgcaac cacttactag cttttattat ggtgcaggaa
    10861 atatagataa tgtaaaaaaa ttattaaaaa tggggataaa agccacaata atagcatcag
    10921 ttgctgtatt tgcaatttgt atggcttttt caggaccaat agtctcttta tttatacatc
    10981 cagaagaaac aatgctattc aatgaaggag ttcgtgtatt taaaatattc tctatatctt
    11041 tcttattggt aggaattaat gtaataattt ctggattctt tgtatcagta gaaaaaccat
    11101 caatttctac tgttatatct cttggtagag ggcttgtaat agttgtttta agtctaattt
    11161 caatgatact aatatttggt ggacaaggaa tatggatgac tactattgtt tctgagttta
    11221 tatgtcttat tttatcatta gtttttctaa aaaagaattt ttctacatta gactctaatc
    11281 taaataaggt tgcttagatg ataagagtga caatatatga aaatatctaa ataaaaaata
    11341 cctattatac aatcttgcaa atattaaacc tttgcaaaaa ttaggattat ataataggta
    11401 ttttatttat gtaattattt tatttctaaa tatccatagc catcttttat taaagttcct
    11461 tttacttcat attctacagg ttttttaaat attggaccat ctacgacaaa tgtatgatac
    11521 tctccatgtt ctccacatgg gtctgcacca gcatttttta tcttctccac aagttctcta
    11581 gttaattttt ttcctaaaaa ttcctctcct aagtgcttta agtttacttt tgttacaaca
    11641 ctacaaaatc cactgtctat aaattcatat actaactcct ctctactctc ttgccataat
    11701 ggtaaatcta ctttcatacc aactgcttca catctatcag tatcccattt tctatgactt
    11761 tctatatcta tatctccata tgcacatata gttgctccca tgtttttagc ttttattaaa
    11821 gccctctcaa agctctcttc atatccttct acaccacact cagcaactaa taaaggtaat
    11881 cctatattgc tacttacttg cttaagcata ttatattcta aattatgtgt ccatgacttt
    11941 ccatgttctt cacttatagt tgtcaagagt gccacaggct tatatccttt tttcaacatt
    12001 cgatttaatg ctaatatact atccttacca ccactaaatg acattacaaa tttttcatca
    12061 gccaatttta acacctcttt catctaactt tgttttatta acataattat accaaatttt
    12121 tgagttgcat agcaaattat attaaattgt taattcacta taaagaatgt ccctaatatt
    12181 aaaagtataa cccctatgta tttgtataaa tgcattgttt cttgtcccaa aattacgcta
    12241 tcaataaaaa atccaactaa aacttgggat acaagagtta atataagtgt atttgatact
    12301 cctaaatttg gaattgcctt tactgtaaaa tatattatta gacctccaaa tactcctcct
    12361 aataacaact tataattaaa accatttata gatactagac actttaagtc acctgtaact
    12421 attatacttg ccaaaaaaaa tattgctcct acagttaagc ttataaaggt ggccactaat
    12481 gctgttgtac tttttcctag ctctccattt ataaaagctt caagagctgt agaaccacct
    12541 gctaataatg caaatattat tgcccataat tttgtcatgg acaaacctcc ttctatataa
    12601 ttctattaat agaagtttat gatacaacct attaattaat tactaagtat agtttaattt
    12661 ttatacacta ggctttattt ggatatgaat ttctgttata attgttctat cgattttgga
    12721 ggtgatttta tgtacacaaa ttttaatatc gattttaata actttaataa aaaagaaaat
    12781 gccattaaat ttatgttaat gggagtatta ctcattatat taggattatt atgccttaca
    12841 tttaagacat taggtataaa attaatttct tggacttttg gtattgcact tttattcttt
    12901 gcatatttaa atttaaaaaa tattaatgaa ctaaaaagat atgctactaa agaagaaatt
    12961 aaaccctcta ttaatatcca atgggttcta ataattgcat gtatattact ttttgtattt
    13021 cctcaaaaaa tacaaagcat attttcatta tttttaggat tttatttaat ctttaatcaa
    13081 ctagtagcac ttgtaaatag taagaataat ccttattcta aatttacaac ttggaatata
    13141 gttaagattc tatttggtat atgccttata ttatctccac tttttttatc tagatttata
    13201 gtatcgataa tgtctttttt cataatatta tttggactag ttctattttt ctctggaaat
    13261 acagcgagaa aatattaatt attttctcgc tgtattttta tattacttaa aatagaattt
    13321 gtttaagtta tctatttgtg gtaatatgtt ttttgtacaa gctcagcgta caattttatt
    13381 agaaataggt gattttttgg ataaatttaa atatgctttt gataataaaa gatatcatac
    13441 ttggaattac tatcttagaa atacttttgg agaaaaagtt tttaaggtat ctataaatgc
    13501 aggatttact tgcccaaaca ttgatggaag tcttggttat ggcggatgta cctattgtag
    13561 caaagaaggc tcaggtgatt ttgctggaaa ccctaaagat aatcttatca gtcaatttta
    13621 taatataaaa gaaatgatgt taaaaaaatg gcctcatgca aagtatattg gctactttca
    13681 agcctataca aatacgtatg ctcctcttga agttttaaaa gaaaaatatg agactatatt
    13741 agaacttgaa gatgtcatag gtctttcaat atccacaaga cctgactgtt tgccagatga
    13801 tgttgtagaa tacttgtctg aactaaataa aagaaccaat ttatgggttg aacttggact
    13861 tcaaacaatt catgattcta catctaaaat tataaataga ggtcatgatt ataaaacatt
    13921 tgttgaaggt gttgaaaaat taaagtctaa aaatataaag gttgtcgtac acattataaa
    13981 tggtcttcct ggtgaagatt ataatatgat gatggaaaca gcaaaggcag ttggtaaaat
    14041 gggtgtagac ggtataaaaa tccatctatt gcatgttata aaagatactc ctatggaaaa
    14101 gatgttacaa aatggtatgt taactcttat ggaacaagat gaatatatca atcttgtctg
    14161 tgaccaatta gaaatacttc ctgaaactat gataatccat agactcactg gtgatggtaa
    14221 gagggatgaa ctagtaggtc ctatatggag cctaaaaaaa tgggaaattt taaataaaat
    14281 tgatgatact ttaaaggcta gaaattctta tcagggctgt aagtttgttt aatacaaaat
    14341 aataactata gaaatggtta tacaaatata gtcctttcta tagttattta gtatttacaa
    14401 aacaaaatat taatttgtac atttatacaa tatataataa aaaatagata gtctaaactg
    14461 tatctactta tattttattt gtactgtcca ctttattcta taaaatttat aaatagttat
    14521 tttttaatac caaatataat ttggtataat ttttgttgga atctacaatg tataccaata
    14581 aatccttttt aagtatataa ttctaaatac tttcagtttt ttcaatctgc tttaacttgt
    14641 tggacttata aaatattctt atttcatcat aagaaagatt aagtgctact actgcagata
    14701 ttaacatcaa catcactcca aataacttcc ctaaggaaaa actttctcct aatatagtgc
    14761 aaccaattat tactgaacca actaattcta ctgatgccac aacacctgca actgacaact
    14821 ctacaccttt agatatacct gctgcataaa aaatatatgc aagtgcagct ggaacaatgc
    14881 ctaacattag catacaagat aaaacatcta aatccttagc ataatttaag atttcccaag
    14941 gtcttgaaga aggaatcata aatatagctc caaataagaa actgtatact aatattgttg
    15001 aacttgaaaa ttcctttaaa gcatttttac taatagttgg cataagtgca tatgctattg
    15061 ctgataatac tccaagtaaa agcccaaaag cgtttaaacc ttgaaaatcc aaacgtccac
    15121 cagtcactgc cataattgca ccaataaaac aaagaataag tgaaaataat ttgcttctag
    15181 taatattttc tttataacat aattttgaaa atattgctaa aaacagtggt gatgtatata
    15241 gtaaaactgc tgcaattgat acacccgcaa tgtcaattgc tttaaaataa cataaattaa
    15301 acattgcctg acatataatt cctattatta ctgaatatat aattcctttt ttgtttatct
    15361 ttaagatttg tggtgtcctt atagatgaat atacgcctaa aactataaat ccaaaaaaca
    15421 atcttgtaaa tgctacttgt tctgatgtca gtccatttcc cattaaaaac tttccaaata
    15481 atccaagtgt agcccataat aaccctgcta ttgctataaa tacataccct ttactttttt
    15541 ctcccatttt aaattccctt ctatttttat ttagtcaatt tacactcttc tttttaagca
    15601 aaaactatgc catcttaaat caatatatat cgactctaaa accctagtct ttccaataat
    15661 actaataaat tttcaatttt tttatattta tttttcgctt actttaagtt gatttagact
    15721 tattttaatt gtaaaatgag ttatatataa cttatttaaa gttaaaatag actgaaactt
    15781 atatataaat atcaaatttt aagtatatta atcaattaaa cctattttaa tatatcatta
    15841 aaaatttagg aggaaaatat aaaaatgaat ttagatttgg aattttatca aaaaatatta
    15901 gaagcttccc atgatgaaat atgtgtttct gatgataaag gaataataat atattgtaat
    15961 aaagcatttg aagaaaatta tggtctaaaa aaagaagata ttttgggaaa aaatgtttct
    16021 tttttagaag actctggata ttcaacaaaa agccccatac caattgtatt aaaaacaaag
    16081 actaaatttt cactggaaca agatactcaa acagggaaaa agcttatcat aactgctacc
    16141 cctatatttg atgaaaatgg acacttagaa tttactgttg aaaattgtag agatataact
    16201 gaacttaata atataaaaaa taaacttgaa gacacaaaaa agcaagtaaa aaaatataaa
    16261 tctgaagtag aaacactata cagaactgca cttagaattg aagatactgt aattatggac
    16321 ggaatagtta tgagaccaat cataaacaca gtaaatcatg tttctaaaac ggatgtaagt
    16381 gtacttttat tgggtgaatc tggaacagga aaaagctcac ttgctagata tatacaccac
    16441 aacagcaata gatctaatgg cccatttata acaataaatt gtgcaactat atctccacaa
    16501 cttcttgaat ctgaactttt tggatatact tcaggtgctt ttacaggtgc aagtactaaa
    16561 ggtaaagttg ggctcgtaga attagctaat ggaggaactc tttttttaga tgaaatagga
    16621 gatataccac aaaatctaca ggcaaaattt ttgcaattaa tacaggatag aacttttact
    16681 ccagttggga gcttaaaaaa taaaaaggtc gatataagaa ttatatctgc tacaaatgtt
    16741 gatttagttt caaaagtaaa agaaaaaaag tttagagaag atttatacta tagattaaat
    16801 gttatagaaa taaaattgcc tcctcttaga gaaagaaggg ataatctagt ggaaattata
    16861 aaatattatt ttaataggta ctcaagtgac ttcaacctaa ataaaactat ttctaaggaa
    16921 gctatggatg ctattgcaaa ttatagattc ccaggcaata taagagaatt acaaaatata
    16981 attcaaaaaa tcttgcttac ttgtacagat aatcatataa caatagatga cttaccaaac
    17041 attcttacaa aaaatataca tattaccaat aatggaaata aaacacatat atcccaaatt
    17101 aataaggtta ttatatctga ctcaaaatct actaattata aaaataaaaa ttttgacact
    17161 ttaattaaag agtatgaaaa aaatataatt ttggatgctt atgaaaaatt tggtagttct
    17221 tataaagtag ccaaacacct tgaaatctca cagtctaaag ccaataggtt aattagaaag
    17281 tatactaata cttaatttta aatttgataa ataaaaactt aacactattt acatattgta
    17341 aatttggcca tttactgtta ttatgaatat ggttgaaatt atttaaataa atactgttga
    17401 cttagtttca tatatttacc attaaacttt tatcataaat atcatatcat aataatatat
    17461 ttaataagtc taataaacta tacgaggtgt aaatatgtta atgaataccc attttattat
    17521 ggcaaaatca atacttgata atatagatga aaataaaaca ttctttataa gcgaaaaaaa
    17581 ttttatatat ggcaatataa agcctgatgc tttttctaaa tataaattaa aaaaacatta
    17641 tttagatgag tcatttaata tgattgtttc taaaataaac tatctatgca gtcttaatat
    17701 agatagtctt tctaaatact tttcagtaag tagacttagt caggaactag gagtcatatg
    17761 tcatttttta tgtgattttt tctgtgtagc tcacagtgaa cgttgggaat ttaagcatag
    17821 cttaaataaa catgttactt atgaaagaga acttggagca tttgcaaaag atgttgattt
    17881 atctaaaatt aaatgtggta ctataaatag tagttttgag aagtttttta ctaaacttta
    17941 tagtgattac aaaaagaaaa ctgattatat gaatgattta aaattttcag cttatacttg
    18001 taatagtgtc ataaattata tacttgactc tattttatct aatacaataa aatcatatca
    18061 tatagcaaat tgcggttaac ttaagaattt ttaaacttat ataaacttat aattgaatat
    18121 aattaaaatg actgctctat agaatataat tttatacttc tctaggacag tcatttttta
    18181 tactaactat ttatctccat tataacatct atggcacttt ttacactatc tactacataa
    18241 tttgcacttt gctttacatc ttcccttgcc ttagacattg caaaactata tttaaatcct
    18301 tctaacatgc aaatatcatt tggagaatcc ccaatagtag caacttcatc tggacttaca
    18361 tttaacgttt ttattacatg ctctatactt cctctttttg acacactttt aggaactata
    18421 tttaaagtat atgttccaga aaaatatatt tctaagactt cactaaactc actttttaga
    18481 taaagttcta ttctggataa ctcttccaaa ttttcagaac atacgcctat atttcctata
    18541 agattatcaa aactacttct tttttcaaaa tctgggtcaa ctataaagtc actagaataa
    18601 tgtctaaaag cagtggtatc actacccaca attacatcac ccttatattt aaaataaagt
    18661 tgtgcatcac ttgactcaaa tctatcaatg acttttttta ctaaattatc atctatcgtt
    18721 cttttaaaaa ttacattata atctttatca tatataaatg aaccattttc acatacatag
    18781 tatccattca tatctaaact atctttgata atattatgtg cttgtttaaa aattctacct
    18841 gtagcaacat taaactgtac tccctttttt tctatttctt caattgctac tatatcttct
    18901 tttgttatgg ttccattttc atatagagtt ccatctaaat cacaaaatat atgttttatc
    18961 ataaattaaa agcctccaaa aacaatttta tattttacta atactttaat caaaataact
    19021 taaagctttt ctaattgtat tctcaattca ttaagcaaat ctctagtagc tcttaatctt
    19081 atctttcttc tatccccatt aaatatattt ttctttacta ttgtttttcc atttatatat
    19141 attgcagtat aaaccagtcc cacaggcttc tctttggttc ctccaccagg tccagctact
    19201 ccagttgtag atatacctat attagcttta agtcctttag caatgccctc agccatttct
    19261 atagctgttt gctcacttac tgctccaaac tcttccaaag tttctctttt tactcctaat
    19321 cttttcattt tagaatcatt agaatatgtt acacaacctt caagaaatac agaggatata
    19381 cctggatagt ttataagaga tgaagaaacc aaacctccag tacaagattc agccactgct
    19441 attgttagat ttttttctac aagtagctta gaaacagcat cttctaaagc agtctcccct
    19501 tcagatatgg aaatatctcc ttcagtatat acatatttac caactctact ttttattttt
    19561 tcttctactg gttttattaa tttaaaagct tctctttcat tttcagcctt tgcagttatt
    19621 cttattgtaa cttctaattc ttttgcatat aaagcaactg ttggattagt ctgctcatta
    19681 attatatcga gaatttctaa ttctagattt gactcaccta ttccatataa tcttaaagtc
    19741 ttagatacta gcatttcatt tgtgaattgt tgtaagtaag gtttgacact ctcattaaac
    19801 atagctttca tttctcttgg aggtccaggc aatactatta taaatttacc atcttttttt
    19861 aaaattgccc ctggagctgt accattatta ttttttagta ctatagcatc ttttggaaag
    19921 tacacttgtt tcatattatt ttcggccatt tttttccctg ttttaacaaa gaaagattcc
    19981 aaaacctcta gtgaaggttt atgtagttct aatttctgac caaagtattc tgcagcagtt
    20041 tcttttgtca tatcatcacc agttggccca agaccacctg tagtaattac aaaatcactt
    20101 ctctttaagc tttcatcaaa acattctaaa agtctttgtt tgttatctcc aacagtactc
    20161 tgatggtata cttctattcc caatgatgca agttctttag ctaaaaattg agaatttgta
    20221 tttactatat ctcccaataa tatttctgtt cctacactta ttatttcagc tttcaaaatt
    20281 tatcactcct tgatttatag ccattaagac tagtcttaag ttcataacaa taattttagc
    20341 aaaaataata acataaataa agttaaaaaa tcatttaaac atatctttaa actagattat
    20401 tattctaaaa ccacacataa tataaacttc gtatatagtg gtactttcat cctcaactgt
    20461 taattcatat aattaagtat gtaaataaaa agtttacttt aaaataataa aaattaaaat
    20521 aggagtgtat aatatgaaaa tagcagttat aatgggtgga atatcatcag agagagaagt
    20581 ttctttaaat tcaggaaaag agatatataa taaccttgat aaaaataaat atgaagttgt
    20641 aaaggtaata atagatgata aaaaagatat atttacaaag ataccagaag atatagattt
    20701 tgcaatactt gctttacatg gaaaatttgg tgaagatgga tgtatacaat caatacttga
    20761 gactatggat ataccatact ctggatgtgg acctctttgc agtgggatgt gtatggataa
    20821 aaatataact aaaaaaatgc ttagagattc aaacctacct actgctccat gggttttagt
    20881 aaaatcagtt gatgaaatag actatgatga aatagataat ataggttatc cagtatttat
    20941 aaaaccaaac tcaggaggtt caagtgtagc cacattcttt attcattcta aggatgaagt
    21001 tgaagaagca gttagaaaag gtctggaagt tgatgaattt gtaatgattg aaaaatatat
    21061 accaggtgga gaatatactt cattcatatt aaatggagaa gtgttcccta caatatctat
    21121 aaaatctgat tcaggattct ttgattatga agctaaatac tcagttgaaa aaggtgcaaa
    21181 ggaagaagtt gtttacttag atgaagaact tcaaaaaaga gtaaacgaaa tttctgagac
    21241 ttgttggaaa atttttaatt gcaaagcata tgtaagagtt gatatgatta taagtgaagg
    21301 tattccatat gttcttgaac taaatactct tcctggaatg actcaaacta gtttaattcc
    21361 tagaagtgct gctgctagag gtattaagta cagtgaacta ttagataaac ttatagaata
    21421 ctctttaaat tagatgaaat aatttgaggt ggtaatttga tattttcaaa tctaaaatta
    21481 aatgataatg aaccaattta tatacaacta aaaaattata taagtgatat gatatcaaaa
    21541 ggactaattc cagacaatag taagctccct tctacaaggg agcttagtca gcttttacaa
    21601 gttagccgaa attctgtagt tttaacttat gaagaattaa aatcagaagg attaatctac
    21661 tctatttcag gaaaaggtac atttgttaaa tcaaagaata agtcttcaaa tactacatgg
    21721 tcattaaact gggattgttt agagaataca tactctaaaa aagccaatga attggacatt
    21781 ataaaaagtg aaattccttg gagctctgac ctgatttcct ttaaaagtat ttctccagat
    21841 ggagatttat tcgacatgga agaattaaaa aaatcctttt taaatagaat ttcactagaa
    21901 ggacataaat tactcaacta tggatatgca caaggttata agccacttat tgactattta
    21961 ctagaatata tgacaaacaa aggtgctgat ataagtaata aagatatact aataacaaat
    22021 ggattcacag aaggctttga tattattatg tcatctttta cccaagaaaa agactacata
    22081 atatgtgaaa atcctactca taatactgct ataaaaatta tgaaatctca taacctcaat
    22141 atcttaggtg tagatataaa tgaagatggt ttggacttca atatgctaga agacaattta
    22201 gttaagtata aaaataaaat aaagtttgca tacattacac cctcttatca caatcctaca
    22261 ggaattgtaa tgactcctga aaataggtac aaattctata atcttatgaa aaaatacaat
    22321 atagcaatta tagaagatgg atttaatgaa gaattactct acaatagttc tcatatattt
    22381 cctatctgtt ccctagataa tatgaataat ggggtcgtat atattggtag tttttcaaaa
    22441 attttatttc ctggaatgag aattggatgg atatttgcag ataaaaaagt aatcaataag
    22501 ctagaaagtg taaaaaggtg cagaaacata catgtatcct ttttagacca aggaatttta
    22561 tttgattatt taagtaatgg aggttttgaa aaatacatta aaaaaatacg taaattctat
    22621 ggagacaaat ttaactttgc atataaatgt ataaaaaaat atataaaaac agaatatatc
    22681 ttgggtgatg gtggtttgca tatatttata aaactaaaaa atatagatac tagagtttta
    22741 ttagaaaaat gttatgaaaa aaatgttata tttatgcctg gagacttatt ttttacagat
    22801 aactctggtt ttgatacttt aaggctcgga ttttctaggt tgtcttttga agacattgaa
    22861 attggaataa aaataattgg agaaactata aatgaaatgt tctaggttaa ctaaatattg
    22921 ttagcctagt ttatttttat gaggtataat atatatagtt tatatgaatc taatactggg
    22981 ggataatatg agtaaaaagt caaaacgaag aaaaattaat aaattaaaat taacgattgt
    23041 aataactgta ttatttcttg ctttattatc tatatatgag atactgttct ctaatcccaa
    23101 caaaaaaggt aatataaaga gtcaagaaac tatgcaaaat aaaaataata caacagatga
    23161 ttctaaaaat aatcaaagct ctaccaaaaa gcaggaagat aataatatca aacctacttt
    23221 ttcatattct tcaataccag ataatataaa aaataaaatg attggtaaat ctatgcctac
    23281 tgatgaacct ataagctttg atagtctatc ttatttaaaa cttacatatt atggatttga
    23341 tgaaaaaact catcaaggag aaatgatagt aaacagtgaa ctagctccag aagtagtgga
    23401 tatttttaag gaattatacg aaaaaaaata tccaattgaa aaaattaagt taatagatga
    23461 ctatgatgct gtagatgaaa aatctatgtc tgataataat acctcatcat tttgttatag
    23521 aactatagct ggtacaaatg tagtttcaaa tcatggtaaa ggacgtgcta tagatataaa
    23581 cccattacag aatcctcaag tttctggaaa tgatgttaca cctaaagtaa gtacagttta
    23641 tgctgatagg tctagcacaa aatttggaat gataaaaaaa ggtgatgatt gttataatgc
    23701 ttttgtaagc agaggttgga gttggggtgg ctattggaaa aacccagatt atcaacattt
    23761 tgaaaaataa tagattagaa ataaaggtga attatatgaa tttaatgaca ttggaaaata
    23821 taagtaaaag ctattctgaa aaaaaattac tagaaaatat atctcttgga ataaatgaag
    23881 gggaaaaaat aggatttatt ggtgtaaatg gtactggtaa atctacctta ctcaaaatta
    23941 ttgcaggagt tgaagaaagt gagactggta atataacaaa aactaatggt attaggatag
    24001 aatatctacc tcaaaatcca acttacaatg aaaattcaac tgtacttgaa caagtattta
    24061 atggaacttc tgaagaatta aagctacttg gtgaatatca agaaatccta gaaaaactta
    24121 attcaaactt tacagaggaa ttaaataaaa aattattatc agtacacgaa aaaatagatg
    24181 ccttaaatct ttgggattta gaaagtgaag ctaagtcagt acttacaaag cttggtataa
    24241 atgactttaa tcaaaaaata gcagaacttt ctggtgggca aagaaagaga gtctctctgg
    24301 catcagccct tattacacct tgtgagtttt tgatactaga tgagcctaca aaccatctag
    24361 acaatgatac tatagattgg cttgaggaat atcttaattc cagaaaaggt tcattgctta
    24421 tgataactca tgatagatac tttttagacc gtgttacaaa tagaattatt gaactggaca
    24481 aaggtagatt attcagttat gatgggaatt attcaatatt tttggaaaag aaaatggaaa
    24541 gactttcgct agaagcaagt attgaagata aaagacaaaa tttaataaga acagagcttg
    24601 cgtgggtaag acgaggagct caggctcgta gcactaaaca aaaagctcgt ttacaaagat
    24661 ttgatgaact tataaataga gattctttta aacctgatga aaatctcgat atatctgttg
    24721 catcaagtag acttggaaat aaaataatag aaatacacaa tatatctaaa tcatttgaag
    24781 aaaatactgt tatagataat ttagaataca ctttaactcg tacagataga attggtataa
    24841 taggcaaaaa tggtatgggt aaatctaccc ttataaatat acttaatgga gaaatagagc
    24901 ctgatagtgg atatatttca attggagaaa cagttaaaat aggttgtttt tctcaagacg
    24961 actctcatat gaatattgat atgagggcta tagactatgt aaaagaagct agtgactata
    25021 tagaaacttc agatggtact aggattacag cttctcaaat gtgtgaaaga ttccttttta
    25081 atggtactat gcaatatact ttgataggta aattatctgg tggtgaaaga agaagactac
    25141 atcttcttaa aatccttatg acagctccta atgtgcttct tttagatgaa cctacaaatg
    25201 atttagatat tgaaacgctt aaaatattag aagaatacct agacgaattt aaaggtgtag
    25261 tagttacagt atcacatgat agatatttct tagatagaat ttgtaataaa atatttgcat
    25321 atgaaggtaa tggaaaaatt catatctata caggtaatta cagtgattat cttatttata
    25381 gagagattca aggaattgaa tttgaagaat ctaaaaggga atcagttaaa aaagatgact
    25441 ctactacccc aaaaaaagaa aaacctaaaa atgataaaaa gcttaagttc tcatataatg
    25501 aacaaaggga gtttgaaaat attgactctg atatagaaaa actcgaatct aaaattgcaa
    25561 cacttgatga aagtactgca aaattttcaa ctgattttac taaactccaa gaaattttag
    25621 atgagaaatc taaactagaa aaagaacttg aatacaaata tgaaagatgg gaatatttaa
    25681 ataatctagc tgatgaaata gctaataata agtaatatta aaataatctt aagtttaata
    25741 agttttgctt taaatctgta atttataaat attttaaagg aagtgtctca aaatgaactt
    25801 tttagtttta tgagacactc ttttttatga gaaatcaaat atattttcat catttcaaca
    25861 atacaaaaga ggcttatctc tattttgaga cagcctcttt cataattacc ttctaattta
    25921 tatgctctaa tatatctata ataattctta ttatatctat atatccagtc tttcaatata
    25981 gtgacaatta acttcaatat gatttaaaaa tactaacaac ctttcataaa gtggatgaga
    26041 ttcttctcca tatttagctt ctaggttctt gcctatttct ctaacagtcc tttgaccatc
    26101 aatttgtaaa aaaacaaaac tggaatattc atctagttct atttttttgt atttaggtat
    26161 tctagctttt aactttctaa aaaacttttg aattttatgg tcctgttttt caagtatagt
    26221 aacaacacga ttttcagcta cttcatattc aagtccatca gtaattttaa atacaatatc
    26281 taaaacatca ttactcttgc tcattatttt ttactttaga taaaacaata ggtaatgctg
    26341 taagcacaac taaaactact aataaagcaa ttgccattga atttgttgca gcaaatccac
    26401 ttggtacctt tggagttaca actccagtta cttgtaatat tatacctatt aaaccaatga
    26461 tagaaccacc agcaacaaga ccagaagata aacttatacc attagaaact ttaacttctt
    26521 tttctttttc agttttagat actaattcaa caaataaacg aattaatgct ccaactaata
    26581 ttatagaagt agttgcaatt ggtaaataga atcctatagc tatagtcatt attggtaaat
    26641 ttaatgcata tagaactatt gccataaata ctccaacaat aatcataacc caaggtaaac
    26701 tacctgacat aataccagaa gttaatgttg acattaagtt tgcttgtggt aatgcaaatt
    26761 gtacattatc accagttgct ctaagttggt cagaaagtaa taaaattact cctacaacaa
    26821 ctataacacc aactatactt gcaattgtaa agtaacgttg catttcgttt ttgctaccac
    26881 caataatata agttactttt tgagattgag tataacctcc agctatagca atagcaacaa
    26941 ctataaatga accaaataaa agtaatgatt tattagcttc taaatcagtc catcccatta
    27001 taacaaacac taatgttaca ataactagag aagctatagt cataccagaa actggaagat
    27061 ttgatgttcc gattgttcct gttaaacgac cagctactat gacaaataat aaagataata
    27121 aaagagatat gatagctcca ataattgcca taacaatgtt accagaaatt aagaatgctg
    27181 ctaaaaatcc aataactact ccacctaata aaagtatcat ttgtatagat gaaccttctt
    27241 ctccttctcc agcacttgat ttagctttaa gtgtttcttt tatagaagcg attatagttg
    27301 gtataagttt tattgcacca ataatacctc cacaaagcat cataccagca cctatatatt
    27361 ttacataact actagatatt gctccaacat ccatttggtt aagaggcatt gcaggattat
    27421 tccataccat agctccatct ttagccatat ctgtaaagta tcctattaaa ggtgctatac
    27481 caaagtttga taaaatactt cctgcaaaca ttgttaaaga aacttcaagc cctactataa
    27541 atccaattcc taaaagtaga ggatttactt caatatcaaa tttccatttg tagaatttgc
    27601 ttcctacaag actcattaca ttgtttgcta catttaagaa tgaaccagtt aatacagtaa
    27661 taaatccact tattacaaat ccaattccca tgtacttaat tgaatctcca ccagcctctg
    27721 aagcaacaag tgtctcagat atagccattg attctgggta cataagttta ccatgttctt
    27781 caactattag atagttgtga actatagcag caattcctat accaaataaa actccaccaa
    27841 ctccaacagc taagccttct aagaatgtaa tttggcttcc aattaatata actgctggta
    27901 atacgaaaat aaatccactt gcaacagatt ctccaccact tgacatacct tggattaagt
    27961 ttttaccaag aatacccttt gaacgagcaa atgctccaac aaaagcagaa cctattatag
    28021 ctcctggaat acctgcggca actgtaagac cggctttcat tccagagtaa gttgtagatg
    28081 cggcaaaaat ggcagctaaa ataataccga taatcagtac agctacattt ccaccggttc
    28141 tggacttatc tgtgatatat ggaacgtaat cttttccact aacctcacca taagcaccct
    28201 taggtaattt tttgttcata aaattttcac cccttttcaa tttgtatata ttataacata
    28261 tatacatata aaacttaact ttttattatt ttacaaatag cgagtcaaaa ctactaaaat
    28321 ttaaccctat attattaatt tctactttta gatattttta tgctaatttt ctttttattt
    28381 ttcaattcta ctaaaaacta ttaagatata aaataaaaaa attttttaat gtaaaattaa
    28441 ttttgtaaaa aaaatgcttt aaaacacgga aaagtataaa aaatacaata atcattctta
    28501 taaataaatt ttcaccatag taaatatata cagaaaattc ttgtttttat gcaaattgat
    28561 tattttataa aaataagtgt cataaagaaa tgtctgaaat cactttatga cacctatcat
    28621 aataaaaaaa ttattctggt aatgctcttt tatttacttt tccgattggt gtaactggta
    28681 tcttatctat cctcattatt attgttggaa tcatatatgg aggtagatgc tcatttaaat
    28741 attctctaat agttctttca tttatcttac cactatcttc ctcatcaatt acataatatg
    28801 cacatatata aatatctcca tcatcatttg ttctcgatat acaaacaaca cttttaattt
    28861 ctgtaatatt atttattata ttttcaatct catatagctc tatcctaaac tcatcaattt
    28921 ttacttgatt gtccatcctt ccaatataat ctaaatttcc atcatctaac catcttacta
    28981 aatcaccagt tctatacatt aacttattat gttcacaagt tttgtaagga ttctctacaa
    29041 atacttgctc tgttagccct tttttattta aatatcctct tgatatttga atgcctgata
    29101 gacatagctc tcctggtgct ccaattggac atagtttgtt ctttttatct aatacatatg
    29161 cataagtatt aaatataggt tttcctattg gtatttttcc atattctctg tccacatgaa
    29221 aagaagtcga tacaacagta aattcggttg gtccatactc attatatagt tcataattac
    29281 gctttctata tccttttaat ctttctcctc cagtctgtac aactctaagt gaattattat
    29341 tgcaattttg tataaataac cttgcaaatg ctgttggcaa tgctagtata gttatgtcat
    29401 tttcctcaca gtattcattt actgtatatt catcaaattt agcttcttca ggcataatat
    29461 ctattgatgc acctacaagc aaataaggga atagttgttt cattacagca tcaaaactga
    29521 aacttaagta tataccacat atatcatttt ctgtagcgct tatagatttt acgctataat
    29581 aacacatatt tactaaactc ttatgttcta tagctatggt ctttggccta ccagttgacc
    29641 ctgaagtaga tatcgtatat gcacaatttg atgatgttat tttttcagtt aattcatatc
    29701 caaacttaaa catttcttca tcatttatat cgagtactat ggtattctca tttacaacct
    29761 tacttttgag ttcatctgta gttatcacta ctttcgcatt actatcttca agaaggtaat
    29821 tcaatctttc ttctggatta cttgtgtcta ttggaaaaaa tgctccccct gatttcataa
    29881 tccctattgc tgtaataatt atgttaatat ctcttggtaa cattacagct actatgtctt
    29941 cactttccac tcctatatga tttaaataac ctgcaagttt atcagttact tcatccaatt
    30001 ctttatatgt taactttcta ttttcatccc tagcagctat attactaggt gttaccttaa
    30061 cttgctccct aaataaatca acataacttt tatcataggg tacttcatat tgattattat
    30121 taaagtcttc caataatact ttttcttctt ccttacatac aaattctaaa tcttctatta
    30181 aaatatcatt atcttctatt atttgctcta ttatataatc aaattgattt aaaattcttc
    30241 taatagtttc tcttttatat atagatggtt tatactttat ttttatttta agtgccctct
    30301 tagtatatac tattacagat aaatcatatc cttttttttc tatgtcataa tcatatgata
    30361 aatcaaaact tttagctata aatattgtgt ttataagctt agtatttaga tgatttttat
    30421 tctctatatc tgataacaaa tagtatccat actctaaaga cctgctccac tgccttttta
    30481 gcttccttac aacatctata aatttttcac tttgtctaac tcttactctt attggaatta
    30541 tattcattgt tttaccaaaa actacatcat cttcatatat atacttttga agtataaggc
    30601 catatatagt ttctatgata gagtctatgc taatattgtt cactttagaa tattgaatta
    30661 acctttcatg tttctcaata ttcatcttaa aaacctcatc tactacttca tcagaacaat
    30721 ctttataaaa aaaatcattt ggaatttttg ctatattcgt ataatcatct aacaaatcca
    30781 tccaataatt catagctcgt tctaaattaa cttcatacat atcaaaattc ccccttacta
    30841 ctaagtctat agaaaaaatt cttttggaat tttattttca caatatataa atatattaat
    30901 aatacatatg caagctctaa aaacatgagc ttgcataaaa ttacacttat tatgactact
    30961 aaattcgtaa tatttacaat gtttaaaata tattataaca catatatgat tttatttttc
    31021 tacat
//
LOCUS       DAMJYR010000037        30678 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.037, whole genome shotgun sequence.
ACCESSION   DAMJYR010000037 DAMJYR010000000
VERSION     DAMJYR010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 30678)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30678)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30678
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.037"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            153..2054
                     /locus_tag="PC471_001673"
     CDS             153..2054
                     /locus_tag="PC471_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HDF2788805.1"
                     /translation="MRERIILETLKNSSGYLNIDYFADKLGVSTRTIRNEIKKIETIK
                     ECNGFKLEYKSKLGYILNIKDQDKFEHYLRNLPSDLVENPEQRLESIIVELLVNEGYK
                     TIEQLSKKFLVSSSQIKNDLKKIDEKIKDTELKLERKAHYGIKIEGSIKEIQSILVDS
                     YFRGNRNITEYRNKFIDNLKLANIRSTIKNVLNEHDLEANLTELEEILAQIIILYIRV
                     SMRVVGNASDLKLYTEDLIIDKLLDKIFRDKKYNLNYDEKYYLKELIKLKTKDKKATI
                     KNIDKNKLQDIMFEFFREIDKKYNSNFLEDKEFFNLFYLHIACLIERIKKNHKIINPF
                     SVKISQQYPTVFNLSIQFSKIIENEYHIKISQDEIGFIATHIAVPFEKREEANFNKKY
                     KIAIICSSGGGSAFLINLRLKEIFPNAEIRNFSLLEEKAVIEFAPDLIFSITNLLFEI
                     NAPVILINEILDELDYLDIKESVRFADHIGNISSPKQYILGLFDKNHFRCIKDKAKYK
                     DILDSMSKCIVDEGACSPTYPKDVWERESYLSTIYTNGVAIPHPIEMTGNKNIISVAL
                     IQTDIIYENRVPKIIFMISLIKGNLELHKQISKYLSKIMANKDMVDMLNKSQSYEEFM
                     YKLKIYLGG"
     gene            2057..3100
                     /gene="ypdE"
                     /locus_tag="PC471_001674"
     CDS             2057..3100
                     /gene="ypdE"
                     /locus_tag="PC471_001674"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HDF2788806.1"
                     /translation="MNIEFFKRLSEADGIASNEEEVRHVLLEELKAYSDKIICDGLGS
                     IIFSKIKDESAPNVMICAHMDEVGFMVRSIDKLGMIHLITIGGVKPLAQFVQKVRITT
                     KEGKKIPAVINATYNNGKAENIYADIGAYTEEDVYNLGINVGDMVTYTTSFEEFTLPD
                     RLVGKAFDDRIGCFVMGEVLKELRKENLNCNIHFAATSSEEVGIRGAKTSTQLINPDI
                     VFVIDVACAKNEFVRDYTNKKQIDKGVMLMHRDRTLVPSRKMIDYVMEIADKNNIPLQ
                     HDMFESGGTDGGEAHLVNEGKPCVVTCVPVRYGHCAFSIVSNKDLENIIRLYTQLILN
                     FDEEQYKHIRNFI"
     gene            3115..3420
                     /locus_tag="PC471_001675"
     CDS             3115..3420
                     /locus_tag="PC471_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HDF2788807.1"
                     /translation="MSTEVQIMELISTAGESKSKAFEALKKVKSKDFKGAKLLIEEAR
                     KIDIEAHRIQTELITQDLNEDVESPAMSLLMVHAQDHYMTSQLAKDLIEALIYCLDK"
     gene            3517..3852
                     /locus_tag="PC471_001676"
     CDS             3517..3852
                     /locus_tag="PC471_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDF2788808.1"
                     /translation="MKILLCCAGGFSTNMLMQNMKKVVKESAKLNEKDFEFTAIPADS
                     IESEVDDWDIVLIGPQIAHKIDFLKGILEPKNKPFAVIDKDVYGKMDGATVMKLALIT
                     YKKHQLAQG"
     gene            3884..5206
                     /locus_tag="PC471_001677"
     CDS             3884..5206
                     /locus_tag="PC471_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDF2788809.1"
                     /translation="MFSKFEAFMNRVFMPLAHKVDNQRHLGSIKAGMVAMTPFTILGS
                     IFAILPALPNMLGENNPISKFILSNSAILDLPVSLSIGLIGLYACMCIAFNLGNYYKL
                     YIPGCVALSTFSFLFLVASFTPDGALEIGNLGAKGLFTGMIAGLGSVELYNFCNKKNI
                     TIKMPENVPDFVSRSFELIPTTIIVCGTFIVARFISLKVFGELPPQILTQFLSPLVGS
                     MDNPWSVLALNFAICTIFFFGIHSSVFSPITRPIMITFIAENIAAMQAGETIPHFFTA
                     GASSAFFGFTGCGISIGCVIACMLSKNASYKKIGRVSLFPALFGINEPILFGAPIILN
                     PIMFIPHVFGGAIIGTLPMFFMHWGWLAKPVFDPPYVGVFLEGFLTNGDWHSILANAL
                     QLVLAVLLYWPFFKIMERQQNNKKQKEETKNIFSKEEEGLLDDLGLDF"
     gene            5216..6286
                     /locus_tag="PC471_001678"
     CDS             5216..6286
                     /locus_tag="PC471_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HDF2788810.1"
                     /translation="MNNTMERLVSLIYKRGVDALLIKAKNTKRYIGALSGSGVYVLVT
                     KDKNYQILDGRYIDEADKKTTGFIKRIVSQGSYIPTIIELLKELNIKKLAIENQAMSI
                     QEYLSLKNEGFEINLITNELWKLRAIKSKEEIKLIKKACEITDEVFDEVISEIKKDMT
                     ELEVSALIQYHALKKGASGMSFETIVVSGERGAMPHGRPTNKKLKVNEAITIDFGVVY
                     QGYQSDMTRTISIGKPPKIIKEIYDVVLEAQLSAIESIKEGTRASDVDKVARKIIDKH
                     GFGEYFNHGLGHGIGLGDGEVPTLNPNSEDILVEGMVMSCEPGIYIPNVGGVRIEDDI
                     VIIDGKGVPLNKTSKEFIILGE"
     gene            6291..7478
                     /locus_tag="PC471_001679"
     CDS             6291..7478
                     /locus_tag="PC471_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HDF2788811.1"
                     /translation="MKYNFNKVLNRKEGNCRKWSNHVIKEKFGLNEDAIPMDLADIDF
                     ECAPAIKESILKRASVGDYSYTFIGDDFYDAIINWNKRRFDVYIEKDWIKLTFGTVST
                     LHYIVQAYTKEGEGVLINTPAYDPFAEAVVNNNRKLCCSSLKLDNNRYYLDLEDMENQ
                     MKYENIRLLIFCSPQNPSGRIWTKEELYQVSELCLKYNVILVSDEIHRDIIFKDYRFV
                     SLWNAHPEIYKNSIICVSPNKGFNLGGLKTSYVLIRDVKIRETLLERLKSNSITSPNV
                     FAIPAIVAAYNESEEWLDAMTSYVEENFEIVYDFFETNIPKAKVMKSDSSFLAWIDVR
                     EVFRNEEESKEFFRHANLTMVVGSYFVKDGDGFIRINVGCPRETLNEALNRIKKTYIS
                     MYC"
     gene            complement(7675..8547)
                     /locus_tag="PC471_001680"
     CDS             complement(7675..8547)
                     /locus_tag="PC471_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HDF2788812.1"
                     /translation="MNILQNENLIIESSSFGAELTKIFSKKYDKNILWDGNKKYWGRQ
                     SPILFPIVGKLFDNETIIEENLYSMTQHGFARDMDFEIVDKGDTFVTYKLTDNESTLK
                     KYPYSFELIINYTLNDNSIEVEWIVKNTDSKDIFFSIGGHPAFNLPFYEQNDFSKHYL
                     EFKSKNDVEKINLNGSFTDDIIPIGKLKNLQLNPEVFKNDALIYTNIDEVSICNNDGS
                     KYVTISMEDFPLVGIWTPYYSETNSTAPFLCIEPWYGLADSINSNKIYKDKKFINKLS
                     KGKVFTASYNINIH"
     gene            complement(8941..9132)
                     /locus_tag="PC471_001681"
     CDS             complement(8941..9132)
                     /locus_tag="PC471_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2788813.1"
                     /translation="MDDFSKLKSLYEDGYRCIYHDCVDNNYTIYLKNFYTEGSETIEL
                     SSESDFSQFKDYIDGLRMS"
     gene            9352..10398
                     /gene="cobT"
                     /locus_tag="PC471_001682"
     CDS             9352..10398
                     /gene="cobT"
                     /locus_tag="PC471_001682"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HDF2788814.1"
                     /translation="MSLLESISKNIYSLDNSSIEKTKQRLDRLIHPTGSLGKIEDICM
                     QLAGIFGNENFDTSKKVIIAFAGDHGVYEEGVAPDPQNITKLQFPNFSKGLCGVGVIS
                     KFVGADVVAVDVGINCDEKLDGVLDYKIRKGTSNMAKGPAMSKQEAIRCLEIGIEIAE
                     QCIEKDYKVIGIGEMGIANTTPSTAIISVISGCDPLEVTGIGAGLKKERLKHKAEVIR
                     KAIEINNPNPTDGVDILSKVGGFEIGSMAGVILGCSANRIPVVIDGFISYAAALIAYK
                     INPKTREYMIASHLSAESGTKRALDILKLDPLLNMDMRLGEGSGAALAFDIIEASNYT
                     YKNMATFDEIDMGR"
     gene            10430..10990
                     /gene="cobU"
                     /locus_tag="PC471_001683"
     CDS             10430..10990
                     /gene="cobU"
                     /locus_tag="PC471_001683"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HDF2788815.1"
                     /translation="MSKIILVTGGARSGKSNFAEKLCFDRNNNTAYIATSIPFDDEMK
                     DRVKKHQENRPRNWNTYEIYEDIYSIIKEIYKEHETVILDCVTLLVNNLMFEHNLNIE
                     ESSQEEINKLEQYIKEQVVKLIEEIEKTNLYFVFVTNELGMGIVPGNKLSRIYADIVG
                     RINQYIASKSNEVYFVVSGIAMKIKE"
     gene            11000..11773
                     /gene="cobS"
                     /locus_tag="PC471_001684"
     CDS             11000..11773
                     /gene="cobS"
                     /locus_tag="PC471_001684"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HDF2788816.1"
                     /translation="MKRFILILQFLTRIPIKLNVGFDDEFYKSIVYFPLVGFVIGILS
                     YLIGWISMLLFEPFIASIIITLAGVLITGGLHIDGLGDTFDAIYSYRDKEKMLEIMKD
                     SRLGTNSLLAIMFVLLLKVGFVYDIISNNSLWVIIFMPMIARLGVMLLTYKTVTPREK
                     GMGNLFIGKLTTSMLITAIIYTLLIVALITKFIFLLPNIVLIKVLGSIIVVFVFIILF
                     KKHIYKKIDGVTGDILGCGIELSELVYLIYIYLLIFMFF"
     gene            11793..12407
                     /locus_tag="PC471_001685"
     CDS             11793..12407
                     /locus_tag="PC471_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HDF2788817.1"
                     /translation="MIRLILIRHALTNDNKKGRLSGHINSCISEEGKLQINKITRYLS
                     NENIDKVYTTPSTRTKDTVEKISKLKLLEIEEKEALREISFGDFEGRTFEEIKIKYPN
                     EFEKMIKEGNNYRYPNGESLIDSYKRVAKEIDNIILENNSNLDTKTILICSHAGTIRN
                     IITHLISGSYKYHWNFKIDNASITVLEIDGGFAVIDKMNFTDFI"
     regulatory      12474..12653
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            12783..14282
                     /locus_tag="PC471_001686"
     CDS             12783..14282
                     /locus_tag="PC471_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobyric acid synthase"
                     /protein_id="HDF2788818.1"
                     /translation="MLQGTASNVGKSVLVAGLCRIFKQDGYTVAPFKSQNMALNSFIT
                     KEGLEMGRAQVFQAEAAKIEPIADMNPVLLKPSGNNKSQVIVRGKVVGEMPSSKYHDY
                     KLELVDVLKETFNSLNSRYDIVVMEGAGSNAEINLKDRDISNMGMAEIADAPVIIVGD
                     IDRGGVFASLAGTMLLLNEDEKRRVKGVIINKFRGKKELLQDGVKMLEDIIKVPVIGV
                     VPYTDIKIEDEDSVTTRFKKKMNKSDIHIEIIRTPHMSNFTDFNIFETQEDVSIRYVD
                     YGESLGNPDILIIPGTKSTIDDLTYIRKSGLESQIKNLHSQGKLIFGICGGYQMLGKK
                     LKDPYHVESEIEEVDGIGLLDTETTFEKEKTTTQVEATIVECISEYMNNLKGKKVKGY
                     EIHMGITKRGNDVRSLDLITKKLDKEVNYTEGSVNKEGNVIGTYLHGIFDEISFTRAI
                     LNNIRKKKGLGELESKVNSFNEFKDKEYDKLADFLREHLDIEKIYEIMK"
     gene            14321..15697
                     /locus_tag="PC471_001687"
     CDS             14321..15697
                     /locus_tag="PC471_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobyrinate a,c-diamide synthase"
                     /protein_id="HDF2788819.1"
                     /translation="MKKILIAGTNSGVGKTTISLGIMQALVKRNMKVQPYKVGPDYID
                     PSYHTFITGRHSRNLDSYMLDDEKIKYIVNKSSKDADISVIEGVMGLYDGFGIDLDNC
                     TSSHTSKVLKSPVILVINGKSMAASSAAMVLGYKELDKDVNIKGVIVNNVKTNSHYQI
                     IKSSIEKYCNVEVLGYFPPNNKFSLESRHLGLVPSVEMKSLREKFYTLADEIEHYIDI
                     DRIIEISESDEFESDFDFQTLIENNKIKEHVNNKSIAIAYDKAFNFYYRENIELLEEL
                     GLEINYFSPLEDVSVPNADYIYIGGGFPEIFGKELEANESMRTSILEAHNNNIPIYAE
                     CGGLMYLGEKLLNQEEQEFNMVGIFSGISKMTSSLKRFGYCIGVAKENTLLSKKGESI
                     KGHEFHHSIFESDEKCVYSMRKERDGNIVEEWDGGYSKGNTLATYLHTHFYNNLNCIE
                     NFLKKGNE"
     gene            15698..16669
                     /locus_tag="PC471_001688"
     CDS             15698..16669
                     /locus_tag="PC471_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein"
                     /protein_id="HDF2788820.1"
                     /translation="MSILSIYIGYVTDLIVGDPYSFPHPVRFIGKLINFTQGIIRKVS
                     KNDRQLKFGGFILWFITVGITYLTTYAIVKLFSFNILLSIVVNSFIIYTTLATKCLKD
                     EALKIYNVLKTEDIEKSRIQLSYIVGRDTTNLSEPEIIRATVETVAENTVDGIIAPMF
                     YAFIGGAPLAMAYKAINTLDSTVGYKNKKYKDIGFASAKIDDIANYIPARISVILMTI
                     GSFFLNYNYRNCFKISIRDRKNHKSPNCAFSEGAVSGALGIQLGGTNVYFGEKVYKPT
                     IGDKLREIDKEDIIKTNKIMYASSFVSMLVFTLIYVLCRMILINLII"
     gene            16701..17771
                     /locus_tag="PC471_001689"
     CDS             16701..17771
                     /locus_tag="PC471_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HDF2788821.1"
                     /translation="MKDLGHGANVDEMARLYGKNPKEIIDFSSNINPNVLPNLERYIL
                     KGLEECRNYPDINYTNLRENISKYIDINPDFIIPGNGATEVIYLLMKSIKKKLAIINP
                     TFSEYRRSAELNNLDIIDLELDLENNFKLNIDIIKENIKRFDSLFICNPNNPSGNVQD
                     LKELVHLLDKHNKVLIIDETFMEFVEDESKYSLVKYIESNKNIFIIKAVTKFFGMPGL
                     RLGYGLTSNTEIMNKIYEHKEPWTINSFADILSNFIFEDKEYIKNSKEYYIEERKYML
                     QELRNIRNIKVYDTDANFILIRIYKKTTKELKKDLFKQGNILVRDASNFIGLDDSFIR
                     VAIKSHEDNKILIENMKNLLGD"
     gene            17773..18660
                     /locus_tag="PC471_001690"
     CDS             17773..18660
                     /locus_tag="PC471_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein"
                     /protein_id="HDF2788822.1"
                     /translation="MKSYGICPASCGEFVQGIIDDEEYLCSYAIDMYSKVYIEEKLVD
                     INLGRYKSRLAIEKVFEKFNLPKKYTKNISLNINSKIPVGKGMASSTADIGATIKATL
                     SLIDKDLSSEEISKLAAEIEPTDSIFIDKNSIFNPLNGTVIKYLGNLTNAKVVILEPN
                     KVLDTMKIRLRQDYNKLKVENKEVIKKSFALLEEGLKKNNLSLVGEACTLSSLANENI
                     EKKEYLNEIIKISKKYGAYGVNIAHSGTVVGILIDKFMNDKKMIDALCESNINSVYNK
                     IYTQNIINGGIKGEIEWNT"
     gene            18648..19280
                     /locus_tag="PC471_001691"
     CDS             18648..19280
                     /locus_tag="PC471_001691"
                     /EC_number="5.4.99.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003724727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin-8 methylmutase"
                     /protein_id="HDF2788823.1"
                     /translation="MEYIKNPMKIEEKSFELIQEIIDEIRPDYKFKNNIEEKIIKRAI
                     HTTADFDYLDILKISEEAVDSIIDALKNNASIYTDTNMALSGINKRKLESLGCKYKCL
                     VADDETVKLAKEKGITRSMAAVEIAAKEEGRKIFVLGNAPTALYKVMEMKSEGRLDLD
                     AVIGVPVGFVGAQESKDEVEKTDIPYIISKGRKGGSNLAAAIVNAILYTM"
     gene            19289..20515
                     /locus_tag="PC471_001692"
     CDS             19289..20515
                     /locus_tag="PC471_001692"
                     /EC_number="2.1.1.195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin-5B (C(1))-methyltransferase"
                     /protein_id="HDF2788824.1"
                     /translation="MEEYVYIDGKKYRRGYTTGSCATGASKAAVYMLITKNRINTINI
                     DTPKGIPLLLKVDNINISDTFVECSIKKDGGDDIDATHTMDIYARAEIVAKNDKNKGY
                     LTLKDIDNLSTNSECKSELYKFIRVYGGTGIGVVTKKGLSVDVGKPAINPTPLKMINH
                     EIRKLIGDNFESILGNDKVLKITIFAPQGETVAKKTFNPRLGIVGGISIIGTTGIVEP
                     MSDEGWKKSLSIELQMKKEQGLDKIILVPGNHGEQFIREKLNLDIKYVVRVSNFIGYM
                     IKEAQRIGYKKILMAGHIGKFIKVSAGIFNTHSKVADARSEILVANLALMGARYEFLN
                     KINQCVTTEEAVELINNSEYREVYNILSNKCRERVKQYLNEDSDDIDVEVIIFSMDKS
                     LLGKSDNTDDLVEVFI"
     gene            20512..21120
                     /locus_tag="PC471_001693"
     CDS             20512..21120
                     /locus_tag="PC471_001693"
                     /EC_number="2.1.1.289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin-7 (C(5))-methyltransferase"
                     /protein_id="HDF2788825.1"
                     /translation="MINIIGLGPGNLDYITKKGENLISTSDVLIGGKRNLESIKNFEG
                     EKIVLDSNLREIIEYINNNKEKQISIIASGDPLIYGIGRYLSKNIDNKMLNMVSGISS
                     LQYIFSKIYVDMNDVYITSSHGKVPDFDYILSHKKVCMVTDSKIGPKQISREIIDRNL
                     NKIIVVGENLSYDNEKITIAKPEEIIRMDNFDMNVVVILDEE"
     gene            21110..21685
                     /locus_tag="PC471_001694"
     CDS             21110..21685
                     /locus_tag="PC471_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating cobalt-precorrin-6B
                     (C(15))-methyltransferase"
                     /protein_id="HDF2788826.1"
                     /translation="MRNSEFITGKVPITKEEVRAISISKLNLVNAKNLIDIGAGTGSV
                     SVEAAYNYPNLKVISIEKNDDAIELIEKNKKKFNLKNIEVIKGYAPINLGLNVKVESI
                     FLGGTGNNLEEIIEWSKKNLVTGGRLVANFIIIDTFNQTLKLLKKHGFKEIDVCVLNV
                     SKLEKLGKGEYFKPLNPIYIISCEKGENDDE"
     gene            21678..22430
                     /locus_tag="PC471_001695"
     CDS             21678..22430
                     /locus_tag="PC471_001695"
                     /EC_number="2.1.1.271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin-4 methyltransferase"
                     /protein_id="HDF2788827.1"
                     /translation="MNKVHFVGAGPGDKELITLKGYKLLSNADVVIYAGSLVNPELLE
                     YCKEDCQIHNSAHMDLQEIIDVMREGIENNKSVVRLQTGDFSIYGSIREQVEDLNKLN
                     IDYDCTPGVSSFLGAASSLGVEYTVPEISQSVIITRMEGRTPVPEKESIQSYAKHQTS
                     MVIFLSVQEIEKVVSKLLEGGYPKDTPIAVIYKATWADEKIVKGTLSDIAVKVKENNI
                     NKTALIMVGRFLGEEYNNSKLYDKDFKHEYRG"
     gene            22414..23535
                     /gene="cbiG"
                     /locus_tag="PC471_001696"
     CDS             22414..23535
                     /gene="cbiG"
                     /locus_tag="PC471_001696"
                     /EC_number="3.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin 5A hydrolase"
                     /protein_id="HDF2788828.1"
                     /translation="MNIEGNIAIICITENGKKLALKINSLVNNCVIYQVKNKKSNLVV
                     EETCVHTVQKKLSDFVGEIFSKFDYIVFIMATGIVVRSIAPYIISKFSDPAILVMDEK
                     GKNVISLLSGHIGGANSLTQYISSLIDANPVITTATDINQKASLDMIAKKLNAHIDNF
                     RESVKEVNSMLVNNKCVGLYVDEGYQVDTRGFKLIESKDELRDSFSNTYIEKLVVVSN
                     KINCIEDIENLNLDNKFEHCYSTQKIIKVIPRDIVIGIGCRRNTDSELMYNSLEEFLN
                     KQNIDIRAINTVGSIELKKDEKAIIELSKILKVPFKTFSVDEISKVDNLFDKSDFVKK
                     NVGVYSVAEPVAYLLSKGNLLVEKHKYKGITFSVGRIKI"
     gene            23532..24257
                     /gene="cobJ"
                     /locus_tag="PC471_001697"
     CDS             23532..24257
                     /gene="cobJ"
                     /locus_tag="PC471_001697"
                     /EC_number="2.1.1.131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011701973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-3B C(17)-methyltransferase"
                     /protein_id="HDF2788829.1"
                     /translation="MIYVVGIGPGSKDTMTLEAIKAIEDSEAIVGYKTYIKLIEEFIQ
                     GKEIIQNGMRQEVDRCKQAVEEAKKGKKVAVVSSGDAGIYGMAGLILELISKEDEDIK
                     VKVIPGVTASIGAAAILGAPIMHDFCHISLSDLMTPWEVIEKRLRLAAEADFVICLYN
                     PRSKGRSEHLSKAFKIMGEFKGGDTPVGVVKDVGREKEEKFVCTFENMDFEKVDMTTM
                     VIIGNKSTYINGELMITPRGYTV"
     gene            24254..25006
                     /gene="cobK"
                     /locus_tag="PC471_001698"
     CDS             24254..25006
                     /gene="cobK"
                     /locus_tag="PC471_001698"
                     /EC_number="1.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-6A reductase"
                     /protein_id="HDF2788830.1"
                     /translation="MILILGGTSDSLSICDKINELKNQPYILSVTTDYGRELAQQHAE
                     NVILGKLGKEDMLKFIKDNNIVKIIDATHPYAVEVSKTAITCAKLLNIDYIRFERKSL
                     IEEIDYDNKFIVDTIEEACEIANKIGTNIFIGTGSKNLNRFIEEISDKNLVVRVLPTS
                     EVILSCEKLGLSADNIVAMKGPFSQYINEETYKHYNIDLVITKESGIAGGFLEKVNAC
                     KTFNIPVIIIKRESINYENVFNDINKIKDILV"
     gene            25025..26503
                     /locus_tag="PC471_001699"
     CDS             25025..26503
                     /locus_tag="PC471_001699"
                     /EC_number="2.1.1.151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-factor II C(20)-methyltransferase"
                     /protein_id="HDF2788831.1"
                     /translation="MKKALLVVSFGTSYHETREKTIDICEDKIKNSLKSHDFFRAYTS
                     NMIINKIKKRDGIEIDNPIQALDKIYEQGYDEVIIQTLHIICGEEFNKLKEQVDGYSS
                     KFKKIVLGRPLLTHIEDYQEAVEAIKHQIPHMESNEAVVFMGHGTLHESHSAYPALEY
                     MLRDNGINAYVGTVEGYPEIEHVIRRLKEGNIKTVNLMPFMLVAGDHAINDMAGDEED
                     SWKTILEENGFNVRVHLKGLGENSYIQDKFVRHAVKCAENAKFYGIGAGPGDGSLLTI
                     KAVNTLKKLDILYTPESKKGGDSLALSIVSEYLPESVEIKSRHFPMSFDGNEKSLAWD
                     NVAQEIISDVNDGKNVGFVTLGDPMIYSTYVYIMKRILGEVEVETIPGISSFSNIASN
                     QNFPLVMDKEALVVIPCTMEDDKIEYALQNYNSIVLMKVYKNFKEIIKKLEKYDLIEH
                     AILVSNSSQESENVFTDLKEAHLEDKISYFSTILVNKDNKIK"
     gene            26533..27438
                     /gene="hemC"
                     /locus_tag="PC471_001700"
     CDS             26533..27438
                     /gene="hemC"
                     /locus_tag="PC471_001700"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HDF2788832.1"
                     /translation="MNIVVGTRGSNLALIQTEWVINELKKKYPEISFEIKIIKTKGDL
                     IQNVSLDKIGDKGLFVKEIEQQLLDGKIDIAVHSMKDMPSYLADGLKFAHTPKREDPR
                     DVLILREGYKNLDDLPHGAVIGTGSKRRKFQLLKQRPDLNIVQVRGNVETRIRKIKDE
                     NMHGIVLAASGIIRANLQDKISSYLPVDVVIPAPAQGALAIEIRSNDSTIEGIVNSLK
                     DENTEIQILAERGFLDGVNGSCHIPMAAYCEIIQDKIHLTGLYGDSEGKKVVIKSIDG
                     DISSPRELGLKLAKLVLKEYENYEG"
     gene            27428..28927
                     /gene="cobA"
                     /locus_tag="PC471_001701"
     CDS             27428..28927
                     /gene="cobA"
                     /locus_tag="PC471_001701"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HDF2788833.1"
                     /translation="MKGKVYLVGAGPGDYKLMTLKGLECIRKSDVIVYDRLANSSYLR
                     EAKPDCELIYVGKASSNHILPQEDINRIIAEKAKEGKIVTRLKGGDPYVFGRGGEEAE
                     TLVDEGIEFEVVPGITSAIGGLCYAGIPITHRDHASSFHVITGHLKGDDSGELNWNAL
                     ANNKGTLVFLMGISNLEKISENLMKEGKDKDTPVALISWATRYNQRVVTSTLENVYET
                     AIKEEVKPPTLIVVGSVVGLREKLNFFESKPLFGKSIAVTRSRNQNSVLVEKIMDLGG
                     NPIEIPTIKVEKIQNNINLENEIKNINKYNYLILTSKNAVEIFFEKIYEMNFDLRILS
                     NLKVCAIGSATSNELKKRGIIADIVPKKFVAESLYEELAPILNDDDNILIPRAENARD
                     YLVDKLKAICEVTEVHIYRTVIDEEKKGEILDILNSNDIDYITFTSSSTVNNLVEIIG
                     KENIKILEKIKTLSIGPITTETINSLGIKLYKESATSTIDSLVDLIIKN"
     gene            28944..29909
                     /gene="hemB"
                     /locus_tag="PC471_001702"
     CDS             28944..29909
                     /gene="hemB"
                     /locus_tag="PC471_001702"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HDF2788834.1"
                     /translation="MLRRPRRLRASKAIRNLVRETKVNVEDLIYPLFVVEGENIKEEI
                     KSLPDVYHLSIDMLEDEIKEISELGIEHIMLFGVPDEKDECASQAFNDNGIVQKAIRK
                     VKEINSDINVITDVCMCQYTSHGHCGILTDTGYVDNDKSLEYLAKISVSHAKAGADMV
                     APSDMMDGRIQAIREALDENGLENVGIMAYSVKYASNYYGPFREAAHSAPSFGDRKTY
                     QMDPANSNEAIIETELDILEGADILMVKPALSYLDIIRRVKDNFDLPLAAYNVSGEYS
                     MLKFAVKEGLLNEDVILESITSIKRAGADIIITYFAKDIAKLLKK"
     gene            30062..30640
                     /locus_tag="PC471_001703"
     CDS             30062..30640
                     /locus_tag="PC471_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional precorrin-2
                     dehydrogenase/sirohydrochlorin ferrochelatase"
                     /protein_id="HDF2788835.1"
                     /translation="MLYPINLKLDELDVVIIGGGEVAYRKCKNFLEFNKNVTIVSKQI
                     LNKFYDLKGNIKIIKDDYKEIYIKDASVIIAATNNRELNMEIGLYCKAKNKLVNVVDN
                     IEISNFTVPSYIKRGDLLISVSTGGKSPSLSSKIKKEIEERYTEDYEEYLNVLGDIRK
                     KVIKKYEDKSKRKDVLNMLVTLDLEELKKFDI"
BASE COUNT        12423 a         3062 c         5670 g         9523 t
ORIGIN      
        1 attttattat tataaaatat ttatattaaa ttgtggaatt aaaatatact aggatataaa
       61 attcttaaat aaaaagaaaa ggttgttttt ataaaaaata ttttttatac cactttaaaa
      121 aacacaataa taacactgag ggaggtgggg atatgagaga acgcattatt cttgaaacat
      181 taaaaaatag tagtggttat ctaaacatag attattttgc agataagttg ggggtatcta
      241 ctcgaactat cagaaatgaa ataaaaaaga tagaaactat aaaagaatgt aatggtttta
      301 aattagaata taagtctaaa ttaggatata ttctaaatat taaagaccaa gataaattcg
      361 agcattatct aagaaactta ccctcagatt tagttgaaaa tccagaacaa cggttggagt
      421 ctataatagt agagctttta gtaaatgaag gttataaaac tatagaacag ttaagtaaaa
      481 aatttctagt aagttcatcc caaataaaaa atgatttaaa aaagatagat gaaaagataa
      541 aggatacaga attaaaacta gagcgaaagg ctcattatgg aattaaaata gaaggttcta
      601 taaaagaaat tcaaagtata ttggtggata gttattttag agggaataga aatataacag
      661 agtacaggaa taagtttata gataatttaa aattagctaa tattagaagt acaataaaaa
      721 atgtacttaa tgaacatgat ttagaagcaa atttaacaga acttgaggaa atattagcac
      781 aaattattat attgtatata agagttagta tgagagtagt gggaaatgct agcgatttaa
      841 aattatatac agaagattta attatagata aattattaga taaaattttt agagataaaa
      901 aatataacct taattatgat gaaaagtatt atttaaagga gcttataaaa ctaaagacta
      961 aagataagaa agccactata aagaatattg ataagaataa acttcaagat attatgttcg
     1021 aattttttag agagatagat aaaaaatata actctaattt tttagaagat aaagagtttt
     1081 ttaacttatt ttacttacat attgcatgtt taatagaacg tataaagaag aaccacaaga
     1141 taataaatcc tttttcagtt aaaataagtc aacagtatcc aactgttttt aatttatcta
     1201 ttcaattttc aaaaatcata gaaaatgagt atcatataaa aataagtcaa gatgaaatag
     1261 ggtttatagc tactcacatt gctgttcctt ttgaaaagag agaagaagct aattttaata
     1321 aaaaatataa aatagcaata atatgctctt ctggtggagg tagtgcattt ttgataaatt
     1381 taagacttaa agaaattttt ccaaatgctg agataagaaa tttttcatta ctagaagaaa
     1441 aagctgtaat agaatttgca ccagatttga ttttttcaat aacaaatctt ttatttgaaa
     1501 taaatgctcc agttatactt ataaatgaaa ttttagacga attggattat ttagatatta
     1561 aagaaagtgt tagatttgca gaccatatag gaaatatatc aagcccaaaa caatatatat
     1621 taggtctatt tgataagaat cactttaggt gtataaaaga taaagctaag tataaggata
     1681 tccttgatag tatgtcaaaa tgtatagttg acgagggagc ttgttctcca acctatccca
     1741 aagatgtatg ggaaagagaa agttatttga gtactattta tacaaatggg gtggctatac
     1801 ctcatccaat cgagatgaca ggaaacaaaa atataatatc agtagctttg atacaaactg
     1861 atattattta tgaaaatagg gtgcctaaaa ttattttcat gatttcatta ataaagggaa
     1921 atttagaact tcataagcaa atatctaaat atttaagtaa gattatggca aataaggata
     1981 tggtagacat gctaaataag tcacaatctt atgaagagtt tatgtataag ttaaaaatct
     2041 atctgggagg ttagacatga atatagagtt ttttaagagg ttgtcagaag cagatggtat
     2101 agcatctaat gaagaagagg ttagacatgt tttattggaa gaactaaaag cttatagtga
     2161 taaaattatc tgtgatgggc tagggagtat catcttttct aaaataaaag atgagagtgc
     2221 accaaacgta atgatttgtg ctcatatgga tgaagttgga tttatggtga gaagtataga
     2281 caaacttggt atgattcact taataacaat aggaggagta aaacctcttg cacagtttgt
     2341 acaaaaagtt agaattacaa caaaagaagg gaagaaaatc cccgctgtca taaatgctac
     2401 atataataat ggtaaggctg aaaatatata tgcagatatt ggagcatata cagaagaaga
     2461 tgtatataac ctagggatta atgttgggga tatggtaaca tatacaacaa gctttgagga
     2521 atttacttta ccagaccgat tagtgggaaa ggcttttgat gataggatag gatgttttgt
     2581 gatgggagaa gttttaaaag aacttaggaa agaaaatcta aattgtaata ttcactttgc
     2641 ggctacaagt agtgaagagg ttggaataag gggagcaaag acatcaacac agttaataaa
     2701 tccagatata gtatttgtaa tagatgtagc atgtgctaaa aatgagtttg tgagagacta
     2761 tacaaataag aaacaaattg ataaaggtgt aatgctaatg catagagata ggactctagt
     2821 ccctagtaga aaaatgatag attatgtaat ggaaattgct gataaaaata atataccact
     2881 tcaacatgat atgtttgaat caggtgggac tgatggagga gaagcacatc tcgtaaatga
     2941 agggaaacct tgtgtagtaa cttgtgtacc agttagatat ggtcattgtg cattttcaat
     3001 agtaagtaat aaagatttgg agaacattat aagattgtat acacagttaa ttttaaattt
     3061 tgatgaagaa caatataaac atataagaaa ttttatctaa aggagaataa gaaaatgtca
     3121 acagaagtac aaataatgga acttatatca actgcaggag agagtaaatc gaaagctttt
     3181 gaggctttaa aaaaagtgaa gtcaaaagat tttaaaggag caaaattgtt aatagaggag
     3241 gctcgtaaaa tagatattga agctcacagg atacaaacag aattaataac tcaagattta
     3301 aatgaagatg ttgaaagtcc agcaatgagt cttttaatgg tacatgctca agaccactat
     3361 atgacttctc agctagcaaa agacttgata gaggctttga tatattgtct tgataagtaa
     3421 ttgtaatata gaaatagatt atttatgtaa aaattctttt aaaagtttag atgcttttaa
     3481 gagataataa aaatacaaac tttaggagga attattatga aaatattatt atgttgtgca
     3541 ggtggatttt ctactaatat gttaatgcaa aatatgaaaa aggtggtaaa agagagtgct
     3601 aaactcaatg aaaaagattt tgagtttaca gctataccag cagattcaat agaaagtgaa
     3661 gttgatgatt gggatatagt acttataggt cctcaaatag ctcacaaaat agacttctta
     3721 aaaggaatat tagagccaaa aaataagcct tttgcagtaa tagataaaga tgtatatgga
     3781 aaaatggatg gagctacagt tatgaagttg gcattaataa cttataagaa acatcaatta
     3841 gctcaaggat aataaaaata atgagggaaa agggagatta gatatgtttt caaaatttga
     3901 agcatttatg aatagagtat ttatgccttt agctcataag gttgacaatc aacgtcacct
     3961 tggttctata aaggctggta tggttgctat gacaccattc acaatattag gaagtatatt
     4021 tgcaatactt ccagcacttc caaatatgct tggagaaaat aatccaataa gcaaattcat
     4081 acttagcaat tcagcaatat tggatttacc agtaagtctt tccataggtt taataggatt
     4141 atatgcttgt atgtgtattg cttttaatct ggggaactac tataaattat atataccagg
     4201 ttgtgttgca ctatcaactt tttctttttt attcctagta gcctcattta caccagatgg
     4261 agctttagaa ataggaaatt taggagctaa agggctattt acaggtatga tagcagggct
     4321 tggttctgtg gagttatata atttttgtaa taagaagaat atcacaataa aaatgccaga
     4381 aaatgtacct gattttgtat ctaggtcttt tgaattaata cctactacta taatagtttg
     4441 tggaactttt atagttgctc gttttatttc attaaaagta tttggagaat tacctccaca
     4501 gatacttact caatttttat ctccattagt aggaagtatg gataacccat ggtcagttct
     4561 tgcacttaac tttgctatat gtactatatt cttttttgga atacattcat cagtatttag
     4621 tcctataact agacctataa tgattacatt tatagctgaa aacatagcag ctatgcaggc
     4681 tggagaaaca atacctcatt tctttacagc aggagcttca agtgcattct ttggatttac
     4741 aggatgtgga attagtatag gttgtgtaat tgcatgtatg ttatcaaaaa atgcttccta
     4801 taagaagatt ggacgtgtat cattattccc tgcattattt gggataaatg aaccaatatt
     4861 atttggagca ccgataatat taaatccaat aatgtttata ccacatgtat ttggtggagc
     4921 tataataggt acactaccaa tgttttttat gcactgggga tggttagcaa agccagtatt
     4981 tgaccctcca tatgtagggg tattcttaga aggattttta actaatggag attggcattc
     5041 tatattagca aatgcattgc aattagtatt agcagtttta ttatattggc cattctttaa
     5101 aataatggaa cgtcaacaaa ataataaaaa acaaaaagaa gaaactaaga atatattctc
     5161 taaagaagaa gaaggcttat tggatgattt aggattggat ttctaatatt ttgttatgaa
     5221 taatacaatg gagagattag tatccttaat ctataaaaga ggtgttgacg ctcttttgat
     5281 taaagcaaaa aatactaaaa gatatatagg agctttatca ggtagtggtg tatatgtgct
     5341 tgttactaag gataaaaatt atcaaattct agatggcaga tatattgatg aagcagataa
     5401 aaaaacgact ggtttcataa aaaggattgt atctcaaggt tcttatatac caactataat
     5461 tgagttatta aaagaattaa atataaaaaa gttagcaata gaaaaccaag ctatgagtat
     5521 acaagagtat ctttcactaa aaaatgaagg ttttgaaatc aatttaataa caaatgaatt
     5581 atggaagcta agggctataa aatctaaaga agaaattaaa cttattaaaa aggcttgtga
     5641 gattacagat gaagtatttg atgaggttat ttctgaaatt aaaaaagata tgactgagct
     5701 agaagtatca gctctaattc agtatcatgc attaaaaaaa ggtgcaagtg gaatgtcttt
     5761 tgaaacaata gtggtatcag gagaaagagg tgcaatgcca catggaagac ctacaaacaa
     5821 aaaattgaaa gtgaatgaag ctataacaat agattttggt gttgtttatc aagggtatca
     5881 atcagacatg actcgtacaa tatctatagg caaacctcct aaaattatta aagagatata
     5941 tgatgtagta ttggaagcac aattatctgc aatagagtca ataaaggaag gaactagggc
     6001 aagtgatgtt gacaaagtgg ctagaaaaat tatagataaa catggatttg gcgaatattt
     6061 taatcatgga ctgggtcatg gtataggtct tggagatggt gaagttccca ctttaaatcc
     6121 aaatagtgaa gatatattag tggaaggaat ggtaatgtct tgtgagccag gtatttatat
     6181 tccaaatgta gggggagtta gaatagaaga tgatattgtt ataatagatg gaaaaggtgt
     6241 tccactcaat aagacatcca aagagtttat aatattagga gagtaaaatt atgaaatata
     6301 attttaataa agtattaaat cgtaaagaag gaaattgtag aaaatggagc aaccatgtga
     6361 ttaaagaaaa atttggctta aatgaagatg caattcctat ggatttagct gatatcgatt
     6421 ttgagtgtgc tcctgctata aaagaatcta tcttaaaaag agcatcagta ggagattata
     6481 gttatacttt tataggagat gacttctatg atgctattat aaattggaat aaaagaagat
     6541 ttgatgtata tatagaaaag gattggataa aattaacttt tggtactgtt tcaacccttc
     6601 attatatagt ccaagcatat actaaagaag gagagggtgt attgataaat acacctgctt
     6661 atgacccttt tgcagaggct gtagtaaata ataatagaaa gttgtgttgt agctcattaa
     6721 aattagataa caatagatat tatttagatt tagaggatat ggaaaatcaa atgaaatacg
     6781 agaatataag attgcttata ttctgtagtc cacaaaatcc atcaggtcgt atatggacaa
     6841 aagaagaact atatcaagtg tcagagttat gtttaaagta taatgtaatt ttggtaagtg
     6901 atgaaattca tagagatata atatttaaag attatagatt tgtatctttg tggaatgctc
     6961 atccagaaat ttataaaaat agtataatct gtgtatctcc taataaaggc ttcaacttag
     7021 gtggattaaa aacatcatat gtattaataa gagatgtaaa aattagggag actttattag
     7081 aaaggctaaa aagtaattca ataacttctc caaatgtatt tgcaatacca gcaatagttg
     7141 cagcatataa tgaaagcgaa gaatggctag atgctatgac tagttatgta gaggagaatt
     7201 ttgaaatagt ttatgatttc tttgaaacta atataccaaa agctaaagtg atgaagtcag
     7261 actcttcttt tttagcctgg atagatgtaa gagaagtatt tagaaatgaa gaagaatcta
     7321 aagaattttt tagacatgca aacttaacta tggtagttgg aagttatttt gtgaaagatg
     7381 gagatggatt tattcgtatt aatgtggggt gtcctagaga aacattaaat gaggctctaa
     7441 atagaatcaa aaaaacgtat ataagtatgt attgttaaat cataaaaaaa gtatctatgg
     7501 tttatataaa aacacattat ttagttatca aatattactg gaattttgta ttccagtaat
     7561 attttttgat taaggaatat tattgaggaa tattaaataa tattatttca tacccttata
     7621 ttaacttata taaaaatatt ttattatttt gggaacgggt tagctaacct ttatctagtg
     7681 tatatttata ttgtaagaag cagtaaaaac ttttccttta ctaagtttat taataaactt
     7741 cttatcttta taaattttgt tagaatttat actatcagca agaccatacc aaggttctat
     7801 acataaaaaa ggagcagtag aattagtttc actataataa ggagtccaaa taccaactag
     7861 agggaaatct tccatagata tagttacata tttactacca tcattattac atatagacac
     7921 ttcatctatg ttggtgtata tcaaagcatc attcttgaat acttctggat ttaactgtaa
     7981 gttttttaat tttccaatag gtattatatc atctgtaaaa gaaccattta aattgatttt
     8041 ttctacatca tttttacttt taaactcaag gtagtgttta gaaaaatcat tttgttcgta
     8101 aaaaggaagg ttgaatgcag gatgtccacc tatagaaaag aatatatctt tagaatcagt
     8161 attttttaca atccattcta cctcaatact attatcattt aatgtatagt ttatgattag
     8221 ctcaaaagaa tatggatact tctttaaagt agattcatta tctgttaatt tataagttac
     8281 aaatgtatct cctttatcta caatctcaaa gtccatatct ctagcaaacc catgttgagt
     8341 catgctatat aaattttctt ctattattgt ttcattatca aaaagtttac ctactatagg
     8401 gaacaatata ggtgattgtc taccccaata ttttttatta ccatcccaaa gaatgttttt
     8461 gtcgtatttt ttagaaaata ttttagttag ttcagcacca aaactgcttg attcgataat
     8521 taaattttca ttttgtaata tattcattta aaatctcctt tattatattt tatgagtata
     8581 ttatactata aagtataaag attattaaat tttatttgat attatatttg aaaggtagat
     8641 tttagtatat tttgaaaatt ttgttttgaa aatttgggat tatttaagaa attttgtatt
     8701 ataaatagaa ggaaatagat aaattgtata gaataaaata tattaagata agttaataat
     8761 gttaaatggg aagtgagttt atggaaaaac caaatacaaa agttgttaac tttcaagagt
     8821 ataaagacag aaaaaaccaa agagaagtaa atagagacga acttttaaag cttataaaaa
     8881 aaattgtgac ttcaaaatag ggagttgtta aactccctat tttaatgggt ttcataataa
     8941 ttaagacatt ctaagtccat ctatataatc tttgaattgt gagaaatctg attcactact
     9001 taattctatg gtttctgaac cttccgtata aaaattctta agatagatag tataattatt
     9061 atcaacacaa tcatgataaa tacatctata tccatcttca tacaaacttt ttaacttaga
     9121 aaaatcatcc acaatatcat ctccttaaag ttcttttaaa tatattttta caaaaagcat
     9181 ctaattgtat gcaaattgac aatgtaaaat aaaggaaatg aacataaaat gtagaataat
     9241 ttttatatgt atgtaaaaat aaaaagaaaa agttgaaatt tttagaaaat aagagtattt
     9301 tttatctaaa aagagagttt aaaaaataaa aaaatgttgg gaggataaaa aatgagttta
     9361 ttagaaagta tatcaaaaaa catatattcc ctagataact cttctattga aaaaacaaag
     9421 cagagattgg ataggcttat acatccaact ggaagcttag gaaagataga agatatttgc
     9481 atgcaattag caggaatatt tggcaatgaa aattttgaca caagtaaaaa ggttataatt
     9541 gcttttgcgg gagaccatgg tgtatatgaa gaaggcgtag cacctgaccc tcaaaatata
     9601 acaaaattac agtttccaaa tttttcgaaa ggtctatgcg gagttggagt aataagtaaa
     9661 tttgtagggg cagatgttgt agctgtagat gtaggaataa actgtgatga aaaactggat
     9721 ggggtattag actacaagat aagaaaaggg acatcaaata tggcaaaagg tccagctatg
     9781 agtaaacaag aagcaattag atgtttagag ataggtattg aaatagcaga gcagtgtata
     9841 gaaaaagatt ataaggttat tggtattggg gagatgggta tagcaaatac tactccaagt
     9901 acagcaataa tatctgtaat ttcagggtgt gacccgttag aagttactgg tataggtgca
     9961 ggtcttaaga aagaaagact aaagcataag gctgaagtaa ttagaaaggc tattgaaatt
    10021 aataatccaa atccaactga tggagtagat attttatcta aagtaggagg gtttgaaata
    10081 ggctcaatgg caggtgttat attaggttgt agtgctaata ggattccagt agtaattgat
    10141 gggtttattt cttatgcagc agcattaatt gcatataaga taaatccaaa aactagagaa
    10201 tatatgatag catctcattt atcagcagaa tcaggaacta aaagagcatt agacatatta
    10261 aaattagacc ctctattaaa tatggatatg agattaggtg agggaagtgg agctgcttta
    10321 gcatttgata taatagaagc ttcaaactat acttataaaa atatggcaac ttttgatgaa
    10381 attgacatgg gaagataaat ataaaaattt aatatgtagg agtgttttga tgagtaaaat
    10441 tattctagta acaggaggag ctaggtcagg gaagagtaac tttgcagaaa aactatgttt
    10501 tgacagaaat aataatacag cttatatagc aacctctatt ccatttgatg atgaaatgaa
    10561 agatagagta aaaaaacatc aagagaatag acctagaaac tggaatactt atgaaatata
    10621 tgaagatata tattccataa ttaaggagat atataaagaa catgaaactg ttatactaga
    10681 ttgtgtaaca ttattagtaa ataatctaat gtttgaacac aatctaaata tagaagaatc
    10741 tagtcaagaa gaaataaaca aattagaaca atacataaaa gaacaagttg tcaaacttat
    10801 tgaagaaata gaaaaaacaa atctatactt tgtatttgta acgaatgagt taggtatggg
    10861 aattgttcct gggaataaac ttagtagaat ttatgctgat attgtaggtc gtataaatca
    10921 atatatagct tcgaaaagta atgaagttta ttttgtagtt agtggcatag ctatgaagat
    10981 aaaggagtag tatgcaatta tgaaaagatt tattttaata ctacagtttt tgacaaggat
    11041 acctataaaa ttaaatgtag gatttgatga tgaattttat aagtcaatag tatattttcc
    11101 acttgtaggt tttgtcatag gtatattatc ctatttaata ggatggattt ctatgctttt
    11161 atttgaacca tttatagctt ctataataat aactctagct ggtgtcttaa ttactggagg
    11221 acttcatata gatggattag gagatacatt tgatgcaata tatagttata gggataaaga
    11281 aaagatgctt gaaataatga aagattcaag attaggtaca aattcacttc tagcaattat
    11341 gtttgtactt ttattaaagg ttggattcgt ttacgatata ataagtaata attcattgtg
    11401 ggttataatt tttatgccaa tgattgcaag gttaggagta atgttattga catataaaac
    11461 tgtgacacca agagaaaaag gaatgggaaa tttatttata ggaaaattaa ctacaagtat
    11521 gttaataact gctataatat acacattatt gatagttgct cttattacta aatttatatt
    11581 tttattacct aatatagtgt tgattaaagt attaggttcg attatagtgg tttttgtatt
    11641 tataatatta ttcaaaaaac atatttataa aaaaattgac ggagtaacag gagatatatt
    11701 aggctgtgga atagaacttt cagagcttgt atatttaatt tacatatatt tgttaatttt
    11761 tatgtttttt taaagtaaaa agtggtgata atatgataag attaatatta ataagacatg
    11821 cactgacaaa tgataataaa aaaggaagat tatcaggcca tataaatagt tgtattagtg
    11881 aagagggcaa actacaaata aataaaatta ccagatatct gagtaatgaa aacatagata
    11941 aggtttatac aacaccatct acacgtacta aagatacagt agaaaaaata tcaaaattga
    12001 aattattaga aatagaagaa aaagaagctt taagagaaat tagttttgga gactttgaag
    12061 gaagaacttt tgaagaaatt aaaatcaaat atccaaatga gtttgaaaaa atgataaaag
    12121 aaggtaacaa ctatagatat ccaaatggag aaagtttgat agactcttat aaaagagtgg
    12181 caaaagaaat tgataatata attttagaaa ataatagtaa tttagataca aagacaatac
    12241 ttatatgttc tcatgcagga acaataagaa atataataac acatttgatt tctggtagtt
    12301 ataagtatca ctggaacttt aaaattgata atgcatctat tacagtttta gaaattgatg
    12361 gtggatttgc tgtaatagat aaaatgaatt ttacagactt catataattt aatcaaaacc
    12421 aaacattaca atctgtgttt gtaatatatc tgtaaaatta gcaatgaaaa tagaatatta
    12481 gattaaaatt aggttcttaa ttagatgtta aaagggaaaa aggttagatg cctttgcagc
    12541 ccccgctact gtgaaaccaa cgaaactata aataccactg tcagtttgat gggaaggtta
    12601 tagaagtagg atgaagttaa gccaggatac ctgcctaatt taatttaaaa caagaggtct
    12661 tcggggtgaa agatttttaa ttaatatata taaaagtcct atctttagaa gtattaggac
    12721 tttttttaat ttgtttttta gtaaatttta acaaggaggg atggaaagtg gggaaaaaaa
    12781 taatgctaca aggaacagct tcaaatgtag gtaaaagtgt cttagtagca ggtttgtgta
    12841 gaatatttaa gcaagatggt tatactgttg ctccttttaa gtcacaaaat atggctttaa
    12901 attcttttat aactaaagaa ggattagaaa tgggaagagc acaagtattt caagcagagg
    12961 ctgcgaaaat agagccaata gctgatatga atccagtttt attaaaacct tctggaaata
    13021 ataaaagtca agttattgta agaggtaaag ttgtaggaga aatgccttct tctaagtatc
    13081 atgattataa attggagtta gtagatgtgt tgaaggaaac atttaatagc ttaaattcaa
    13141 ggtatgatat tgtagttatg gaaggggcag gaagtaatgc agagataaac ttaaaagata
    13201 gagatatctc taatatgggt atggcagaaa tagctgatgc acctgtaata atagtcggag
    13261 atattgatag aggaggcgta tttgcctcat tagcaggaac aatgctttta ttaaatgaag
    13321 atgagaaaag aagagtaaaa ggtgtaataa tcaataaatt cagaggaaaa aaagagcttt
    13381 tacaagatgg tgtaaagatg ctagaagaca tcataaaagt acctgttata ggagtagtac
    13441 cttatacaga tataaaaata gaagatgaag acagtgtaac tacgagattt aagaaaaaaa
    13501 tgaataaaag tgatatacac atagaaataa ttagaacccc acatatgtca aattttacag
    13561 attttaatat atttgagaca caagaagatg taagtataag atatgtagac tatggagaat
    13621 ctttaggaaa tccagatata ttaataatac ctggaactaa aagtactata gacgatttaa
    13681 catatataag aaaaagtggt cttgaatcgc aaatcaaaaa tttacatagt caaggtaaat
    13741 tgatatttgg gatatgtggt ggataccaaa tgttaggtaa aaaattaaaa gacccttatc
    13801 atgttgaaag tgaaattgaa gaagttgatg gaataggtct tttagataca gaaacaacgt
    13861 ttgagaaaga aaagactaca acacaagtag aagctactat tgttgaatgt atcagtgaat
    13921 acatgaataa ccttaaggga aaaaaagtaa aaggttatga aattcatatg ggaattacaa
    13981 agcgaggtaa tgatgtaaga agtttagatt taataactaa aaaacttgat aaagaagtga
    14041 attatactga gggaagtgta aataaagagg ggaatgtaat tgggacttat cttcatggaa
    14101 tatttgatga aataagcttt acaagagcaa ttttaaacaa tataagaaag aaaaaaggtt
    14161 taggagaatt agaaagcaaa gtaaactctt ttaatgaatt taaggataaa gagtatgata
    14221 agttggctga ttttttaaga gaacatttag atatagagaa aatatatgag attatgaaat
    14281 aatgatgacc tttatttata atttaggtag gaggtgtatt atgaaaaaaa ttttaattgc
    14341 gggtactaat agtggagtag gtaaaacaac aatatcatta ggaataatgc aagctttggt
    14401 taagagaaat atgaaagtac agccatataa agtagggcca gactatatag acccttcata
    14461 tcacactttt ataactggaa gacattctag aaatttagat tcttatatgt tagatgatga
    14521 aaaaataaag tatatagtaa ataagtcctc aaaagatgca gatatttcag taatagaagg
    14581 tgttatgggc ttatatgatg gctttggaat agatttagat aattgtacaa gttcacatac
    14641 ttcaaaggta ttaaagtcac ctgttatatt agttataaat ggtaaatcta tggcagcatc
    14701 tagtgctgct atggtacttg gatataagga actagataaa gatgtaaata taaagggtgt
    14761 tatagtaaat aatgtaaaga ctaatagcca ttatcaaata attaagagtt ctatagaaaa
    14821 atattgtaat gttgaagtat tggggtattt tcctccaaat aacaaattct ctttggaatc
    14881 tcgacatcta ggattagttc ctagtgtaga aatgaaatca ctaagggaaa aattttatac
    14941 cttagcagat gaaattgaac actatataga tattgacaga ataattgaaa tatcagaaag
    15001 tgatgaattt gagagtgatt ttgattttca aactttgata gaaaataata aaataaaaga
    15061 acatgttaat aataaatcaa tagctatagc atatgataaa gcattcaatt tttattatag
    15121 agaaaatata gagcttttag aggaacttgg tttagaaata aactatttta gccctcttga
    15181 agatgtatca gtgccaaatg cagattatat ttatattgga ggaggttttc cagaaatatt
    15241 tggaaaagaa ttagaagcaa atgagtcaat gagaacttcc atattagaag cacataacaa
    15301 taatatacct atttatgcag aatgtggagg actgatgtac ctaggtgaga aactattgaa
    15361 tcaagaagaa caagaattta atatggttgg aatatttagt ggaattagta agatgacatc
    15421 ttctttaaag agatttgggt actgtattgg tgtagccaag gaaaatactc ttttatctaa
    15481 aaaaggggaa tccattaaag ggcatgagtt tcatcattca atatttgaaa gtgatgaaaa
    15541 atgtgtatat tcaatgagaa aagaaagaga tggaaatata gtagaagagt gggatggtgg
    15601 ttatagtaaa ggaaacactt tggctacata tcttcatact catttttata ataacttaaa
    15661 ttgtatagaa aattttttga aaaaaggaaa tgaataaatg agtattttat ctatttatat
    15721 aggatatgtg acagacttaa ttgtaggaga cccatattct tttcctcatc cagtgagatt
    15781 tattggtaaa cttataaatt ttactcaagg tataataaga aaggtctcta aaaatgatag
    15841 gcagttaaaa tttgggggat ttatattatg gtttataacc gttggaataa catatttaac
    15901 aacttatgct atagtgaagt tatttagttt taatatatta ttgagtattg tagtgaatag
    15961 ttttataatt tatacaacac tagccacaaa atgtttaaaa gacgaagcat taaaaattta
    16021 taatgtatta aaaactgaag atatagaaaa atctaggatt cagctatctt atatagtagg
    16081 tagggatact actaatttaa gtgaacctga aataataaga gcaactgtag aaactgttgc
    16141 agaaaatact gtagatggta taattgcgcc tatgttttat gcatttattg gaggagcacc
    16201 acttgctatg gcctataaag ctatcaatac attggactca acagttggat ataaaaataa
    16261 aaagtataaa gatattggtt ttgcatctgc taagattgat gatatagcca attatatacc
    16321 agctagaata tctgtcattc ttatgacaat tggaagtttc tttttaaatt ataactatag
    16381 gaactgtttt aaaatctcta ttagagatag aaagaatcat aaaagtccaa attgtgcttt
    16441 ttcagaaggt gcagtatctg gtgctctagg aatacaacta ggaggtacca acgtttattt
    16501 tggggaaaaa gtatataagc caacaatagg ggataagctt agggagatag ataaagaaga
    16561 tataattaaa actaataaaa ttatgtatgc atcttcattt gtatctatgt tggtatttac
    16621 actaatatat gtgttatgta ggatgatttt aattaactta attatataaa ttattgtttg
    16681 ttaacttaag ggggacatag atgaaggatt tagggcatgg tgccaatgta gatgaaatgg
    16741 caagattata tgggaaaaac ccaaaagaaa taattgattt tagttcaaac ataaatccaa
    16801 atgtattacc aaatttagag agatacattt taaaaggatt agaagagtgt aggaattatc
    16861 cagatataaa ttatacaaac ttgagagaaa atatatcaaa atatatagat ataaatccag
    16921 actttataat acctggcaat ggtgcaacag aagtgattta tttgcttatg aagagtatta
    16981 aaaaaaaatt ggctataata aatccgactt tttcagagta tagaagaagt gctgagttaa
    17041 ataatttaga tattatagat ttagagttag atttagaaaa taactttaaa cttaatatag
    17101 atataataaa agaaaatata aaaagatttg atagtttatt tatatgtaat cctaataatc
    17161 cgagtggtaa tgtccaagat ttaaaggaac ttgttcattt actagataaa cataataagg
    17221 tactaataat tgatgaaact ttcatggaat ttgttgaaga tgaaagtaaa tatagtttgg
    17281 ttaaatatat agaatcaaat aaaaatatat ttataattaa agctgtgact aagttttttg
    17341 gtatgcctgg tttaaggcta ggatatggtc ttacaagcaa cactgaaatt atgaacaaaa
    17401 tttatgaaca caaagagcca tggactatca attcttttgc agatatacta tccaatttta
    17461 tttttgaaga taaagagtat attaaaaata gtaaagaata ttacatagaa gaaagaaaat
    17521 atatgttaca agagttgaga aacataagaa atataaaggt ctatgataca gatgctaatt
    17581 ttatcttgat aagaatatat aaaaaaacaa ctaaagaatt gaaaaaagat ttgtttaagc
    17641 aaggaaacat attagttaga gatgcatcta attttatagg attagatgat agctttataa
    17701 gagtggctat aaaatctcat gaagataaca aaattcttat agaaaatatg aaaaatttat
    17761 tgggtgatta aaatgaaatc ttatggaata tgcccagcat cttgtggaga atttgtacaa
    17821 ggtataattg atgatgaaga atatttatgt tcatatgcaa tagatatgta ttctaaagtg
    17881 tatattgaag aaaaattagt agatataaat ttagggagat ataagtcaag gttagcaatt
    17941 gaaaaggtat ttgaaaaatt taatctacct aaaaaatata ctaaaaatat ttctttaaat
    18001 ataaatagta aaatacctgt tggaaaagga atggcaagtt caactgctga tataggagct
    18061 actataaagg caacattatc attgatagat aaagatttaa gtagcgagga gatttctaaa
    18121 ttagcagcag aaatagaacc aacagattct atatttatag ataaaaatag tatatttaat
    18181 cctctaaatg gaactgttat aaaataccta ggaaatctga ccaatgcgaa agttgtaata
    18241 cttgaaccaa acaaagtact agatactatg aaaattagat tgaggcaaga ttataataaa
    18301 ttaaaagtag agaataaaga agtaataaaa aaatcatttg ctcttttaga agaaggattg
    18361 aaaaaaaata atttatctct agttggagag gcttgtactt tgagtagctt agcaaatgag
    18421 aatatagaaa aaaaagagta tttaaatgaa ataataaaaa tatcaaaaaa atatggagca
    18481 tatggagtaa atattgctca cagtggaact gtagtaggta ttttaattga taagtttatg
    18541 aatgataaaa agatgataga tgccttatgt gaatctaaca taaattctgt ctataataaa
    18601 atttatacac aaaacataat taatggagga attaaggggg aaatagaatg gaatacataa
    18661 agaatcctat gaaaatagaa gaaaaaagtt ttgaattgat acaagaaata atagacgaaa
    18721 ttagaccaga ttataaattt aagaataata tcgaagaaaa aataataaaa agagctatac
    18781 atactactgc tgattttgat tacttagata tattaaaaat atcagaagaa gcagttgata
    18841 gtataataga tgcattaaaa aataatgcaa gtatttatac tgatacaaat atggctctta
    18901 gtggtataaa taaaagaaag ttagaatcat taggatgtaa gtataaatgt ttagtggcag
    18961 atgatgaaac tgtaaaatta gctaaagaaa aaggaattac tcgttctatg gcagcagtag
    19021 aaattgcagc taaagaagaa ggaagaaaaa tattcgtttt aggtaatgct ccaacagctt
    19081 tatataaagt tatggagatg aaatcagaag gtagattaga tttagatgca gttataggag
    19141 ttcctgtagg ttttgtagga gcacaagaat caaaagatga agttgaaaaa acagatatac
    19201 catatataat ttctaaaggt agaaaaggtg gcagtaattt agcagcagca atagtaaatg
    19261 ctatattata tactatgtag gtacctatat ggaagaatat gtatatatag atggaaaaaa
    19321 atatagaaga ggatatacta caggttcatg tgcaacaggt gcatctaagg ctgctgtata
    19381 tatgttgatt acaaaaaata gaatcaatac tataaatata gatactccta aggggatacc
    19441 attactacta aaagttgata atataaatat ttctgataca tttgtggaat gttccataaa
    19501 aaaagatggt ggagatgata tagatgcaac tcataccatg gatatttatg caagagctga
    19561 aattgtagct aaaaatgata agaacaaagg gtatcttact ctaaaggata tagataattt
    19621 aagcaccaat agtgagtgta aaagtgagct ctataagttt attagggttt atggcggaac
    19681 tggtattggt gtggttacaa aaaaaggtct tagcgtggat gttggtaaac ctgctataaa
    19741 tccgactcca cttaaaatga taaatcatga aataagaaag cttataggag ataattttga
    19801 atctatactt ggaaatgata aagttttaaa aataacaatc tttgctccac aaggagaaac
    19861 tgtagcaaaa aaaacattta atccaagatt aggtatagtt ggaggaatat ctataatagg
    19921 tacaactggg attgttgaac ctatgagtga tgagggttgg aagaagtctc tatctataga
    19981 acttcagatg aaaaaagaac aaggcttaga taaaataata ttggttccag gaaatcatgg
    20041 agaacagttt ataagagaaa aattaaactt agatattaag tatgtagtta gggtaagtaa
    20101 ttttataggc tatatgatta aagaagctca aagaatagga tataagaaaa tcttaatggc
    20161 aggtcatata ggtaaattta taaaagtatc tgctggtatt tttaatactc atagtaaggt
    20221 agcagatgct agaagtgaga tattagtggc aaacttagct ttaatgggag ctagatacga
    20281 atttttaaat aaaataaatc aatgcgtaac tactgaagaa gctgtagagc taataaataa
    20341 tagtgaatat agagaagtat ataatatatt aagtaataaa tgtagggaga gagtaaagca
    20401 atacttgaat gaagattcag atgatattga tgtggaagta atcatatttt caatggataa
    20461 gtctttatta ggtaaatcgg ataatacaga tgatttagtg gaggtcttta tatgataaac
    20521 ataataggtc taggacctgg taacttagac tatataacaa aaaaaggtga aaatttaatt
    20581 tccacttctg atgtattaat tggtggtaaa agaaatttag agtctataaa aaattttgaa
    20641 ggagaaaaaa tagttctgga ttctaattta agagaaatta tagagtacat aaataacaat
    20701 aaagaaaagc aaatatcaat aatagcatct ggggacccac taatatatgg gataggtaga
    20761 tatctatcta aaaatattga taataagatg ttaaacatgg tttctgggat tagttcatta
    20821 cagtacatat tttcaaaaat atatgtagat atgaatgatg tatatattac aagtagtcat
    20881 ggtaaagtac cagattttga ttacatatta tcacataaaa aagtatgtat ggttacagat
    20941 tcaaagatag gaccaaaaca aataagtaga gaaataatag atagaaatct aaataaaatt
    21001 attgttgtag gagaaaatct gtcatatgac aatgaaaaaa taacaatagc aaaaccagaa
    21061 gagataataa gaatggacaa ttttgatatg aatgttgtgg tgattttaga tgaggaatag
    21121 tgaatttata acaggtaaag ttccaattac taaggaagag gttagagcaa tatcaataag
    21181 taagttaaat ttagtaaatg ctaaaaacct catagacata ggggctggta caggaagtgt
    21241 aagtgttgaa gctgcatata attatcctaa tttaaaggtt atatctatag agaaaaatga
    21301 tgatgctata gagcttatag agaaaaataa aaaaaaattc aacttgaaaa atatagaagt
    21361 gataaaagga tatgctccaa ttaatctagg gttaaatgtt aaagttgagt ctatttttct
    21421 tgggggaaca ggaaataatc tggaagaaat aatagaatgg tctaaaaaaa atctcgttac
    21481 aggaggaaga cttgtagcta attttataat aatagataca tttaatcaaa ctttaaaact
    21541 gcttaaaaag catggattta aagaaataga tgtatgtgtt ttgaatgttt ctaaactaga
    21601 gaaactagga aaaggtgaat attttaaacc actaaatcct atatatataa tatcttgtga
    21661 aaaaggggaa aatgatgatg aataaagtac attttgtagg agcaggacct ggagataaag
    21721 agctgataac attaaaagga tacaaattat taagtaatgc agatgtagtg atatatgcag
    21781 gttcacttgt taatcctgaa cttttggaat actgtaaaga agattgccaa atacataata
    21841 gtgcacatat ggatttacaa gagataatag atgttatgag agaaggtata gaaaataata
    21901 aatctgttgt aagactgcaa acaggtgatt tttctatata tggttcaatt agagaacaag
    21961 tggaagattt aaataaattg aatatagatt atgactgtac tcctggagta agttcttttt
    22021 taggggcagc atcatcttta ggggttgaat atacagttcc agaaatttct caaagtgtaa
    22081 ttataacaag aatggaaggc agaactccgg ttccagagaa agaatcaata caatcatatg
    22141 ctaagcatca gacatctatg gtaatatttt tatctgttca agaaattgag aaagttgtgt
    22201 ctaaattact ggaaggtgga tatccaaaag atactcctat agcagttata tacaaagcaa
    22261 cttgggcaga tgaaaaaata gttaaaggaa ctttaagtga tatagctgta aaagttaaag
    22321 aaaacaacat aaataagact gctttaataa tggttgggag atttttagga gaagagtata
    22381 ataattcaaa gttatatgat aaggatttca aacatgaata tagagggtaa tattgctata
    22441 atctgtatta cagaaaatgg aaaaaaatta gctctaaaga taaattctct agtaaataac
    22501 tgtgttatat atcaagtaaa aaataaaaaa agtaatttag ttgttgaaga aacatgtgtg
    22561 catacagttc aaaagaagtt aagtgatttt gtaggagaaa tatttagcaa atttgattac
    22621 atagtattta taatggctac aggtatagtt gtacgaagta tagcaccata cataataagt
    22681 aagtttagtg accctgcaat tttggtaatg gatgaaaaag gcaaaaacgt aataagttta
    22741 ttgagtggtc atattggagg ggcaaacagc ttgacacagt acataagtag tcttattgat
    22801 gctaatccag taataacaac agctactgat ataaatcaaa aagcttcctt agatatgata
    22861 gcaaaaaaac taaatgcaca tattgataat tttagagaaa gtgtaaaaga agtaaattct
    22921 atgctagtga ataataaatg tgttggatta tatgttgatg aaggatatca agttgatact
    22981 aggggattta aacttataga aagtaaggat gagttaagag atagtttttc aaacacatat
    23041 atagaaaaat tagttgtagt aagcaataaa ataaattgta ttgaagatat tgagaattta
    23101 aacctagata ataaatttga acattgctat agtacccaaa aaataataaa ggtaattcca
    23161 agagatatag tgataggaat tggctgtaga agaaacacag atagtgaact gatgtataat
    23221 tcacttgaag agtttcttaa taaacaaaat atagatatta gagcaataaa cacagttgga
    23281 agtatagaac tgaaaaaaga cgaaaaagca ataattgaat tatcaaaaat attgaaggtg
    23341 ccatttaaaa cattttcagt agatgaaata tctaaggttg ataatctttt tgataaatct
    23401 gattttgtta aaaaaaatgt gggtgtgtat tcagtagcag aacctgtagc atatcttttg
    23461 agcaaaggta atttgttagt tgaaaaacat aaatataaag gaataacttt ttcagtgggg
    23521 agaataaaaa tatgatatac gttgtaggaa ttggaccagg aagtaaagat acaatgactt
    23581 tagaagcaat aaaagcaata gaagattctg aggctattgt tggatataag acttatataa
    23641 aactaataga agaatttata caaggtaaag aaataatcca aaatggtatg aggcaagaag
    23701 ttgatagatg taaacaagca gtggaggaag caaaaaaagg aaaaaaagta gcagttgtaa
    23761 gcagtggtga tgctggaata tatgggatgg caggtcttat tttagaatta atatctaaag
    23821 aagatgaaga tataaaagta aaggtaattc caggcgttac tgcaagtatt ggtgcagcag
    23881 ctatacttgg agctcctatt atgcatgatt tctgtcatat aagtttgagt gatttaatga
    23941 ctccgtggga agtaattgaa aaaagattga gattggctgc tgaagccgat tttgtaattt
    24001 gtttgtataa tccaagaagt aagggaagaa gtgaacatct aagcaaagca ttcaaaataa
    24061 tgggtgagtt taaaggtgga gatacacctg ttggagtggt aaaagatgta ggtagagaaa
    24121 aagaagaaaa gtttgtatgt acttttgaaa atatggactt tgaaaaagtc gatatgacta
    24181 caatggttat aataggaaat aagtctacct atattaatgg tgaattgatg ataactccaa
    24241 gaggttatac agtatgattt taattttagg aggaacttca gatagtttat ctatatgtga
    24301 taagataaat gaactgaaaa atcaaccata tatcctttca gttactacag attatggaag
    24361 ggaacttgca caacaacatg cagagaatgt aattttaggt aagcttggaa aagaagatat
    24421 gctaaaattt ataaaagata ataatatagt aaaaataata gatgccactc atccttatgc
    24481 agttgaagta tccaaaactg ctattacttg tgcaaaacta ttgaatatag attatataag
    24541 gtttgagaga aaatctttaa ttgaagaaat agattatgat aataaattta tagtagatac
    24601 aattgaagaa gcctgtgaaa tagcaaataa aatagggaca aatatattca taggaacagg
    24661 aagtaaaaat ttgaacagat ttatagagga aataagtgat aaaaatttag ttgttagagt
    24721 tcttccaacg agtgaagtca tattaagttg tgaaaaatta ggtttaagtg cagataatat
    24781 agtagctatg aaaggtccat ttagccagta tataaatgaa gaaacatata aacattacaa
    24841 tatagattta gtcataacaa aagaaagtgg aatagctggt gggttcttag agaaggtaaa
    24901 tgcatgtaaa actttcaata taccagtaat aattataaaa agagaatcga ttaactacga
    24961 aaatgtattt aatgacataa ataaaattaa agacatactt gtgtaatttt taggaggaga
    25021 atatatgaag aaagccttat tagtagtgag ttttggtaca agctatcatg aaacgagaga
    25081 aaagacaata gatatatgtg aagataaaat aaaaaattcg ttaaaaagcc atgatttttt
    25141 tagagcttat acatcaaata tgataataaa taaaataaaa aaaagggatg gaatagaaat
    25201 agataatcca atacaggctt tagataaaat atatgaacaa ggatatgatg aagtaataat
    25261 ccaaacactt catattatat gtggcgaaga atttaacaaa ttaaaagaac aagtagatgg
    25321 atattcttct aaattcaaaa agattgtatt aggaagacct ttgcttacac atattgaaga
    25381 ttatcaggaa gctgtagagg caattaagca tcaaattcct catatggaaa gtaatgaagc
    25441 agtagtattt atgggacatg gaacacttca tgaatcacat tcagcgtatc cagcattgga
    25501 atacatgctt agggataatg gaataaatgc ttatgttggt acagttgaag ggtatccaga
    25561 aatagaacat gtaataagaa gattaaaaga aggaaatata aaaactgtaa atttgatgcc
    25621 atttatgtta gttgcaggag accatgctat aaatgatatg gcaggagatg aagaagattc
    25681 ttggaaaaca atattagaag aaaatggatt taacgtaaga gttcatttaa aaggactagg
    25741 agaaaattct tatatacaag ataaatttgt tcgtcatgct gtaaaatgtg cagagaatgc
    25801 gaagttctat ggtataggag cagggccagg agatggttct ttacttacga taaaagcggt
    25861 taatacttta aagaaactgg atattctata cactccagaa tcaaaaaagg gtggagatag
    25921 tctagcttta tcaatagtta gtgaatattt acctgaatca gtggaaataa agtctaggca
    25981 tttcccaatg agttttgatg ggaatgaaaa aagtttagct tgggacaatg tggcacaaga
    26041 aattatatca gatgttaatg atggtaaaaa tgtaggattt gttacattag gagaccctat
    26101 gatttatagt acatatgtat atataatgaa aagaatatta ggagaggtag aagtcgaaac
    26161 tataccagga atttcatcat tttccaatat agcatctaat cagaattttc cattagtcat
    26221 ggataaagaa gctttagtgg taattccttg tactatggaa gatgataaga tagagtatgc
    26281 tttgcaaaat tataattcaa tagtattgat gaaagtatat aaaaatttta aagaaataat
    26341 aaaaaaatta gaaaagtatg atttaataga acatgctatt ttagtaagta attcttccca
    26401 agaaagcgaa aatgtgttta cagatttaaa agaagcgcac cttgaagata agatatctta
    26461 tttttctact atactagtaa ataaagataa taaaataaaa taggtgaaag caatatttaa
    26521 aggagactta atatgaatat agtggttgga actaggggta gtaatctagc gcttatacaa
    26581 acagaatggg taataaatga attaaaaaag aagtatccag aaattagttt tgagattaaa
    26641 atcataaaga caaaaggaga tttaattcaa aatgtttcac tggataaaat aggtgataaa
    26701 ggattgtttg tcaaagaaat agagcaacaa ttattagatg gaaaaataga catagcagta
    26761 catagtatga aagatatgcc ttcttattta gcagatggac taaaatttgc tcatacgcct
    26821 aagagagaag acccaagaga tgttctaata cttagagaag gatataaaaa tttagatgat
    26881 ttgcctcatg gagcagtaat aggaacagga agtaaaagaa ggaagtttca attattgaaa
    26941 caaagacctg atttaaatat agtacaagtt agaggtaatg tggaaactag gatacgaaaa
    27001 ataaaagatg aaaacatgca tggtattgtg ttggcagcat caggaataat tagagctaat
    27061 ttacaagata aaataagttc ttatttacct gttgatgttg ttattccagc acctgcccaa
    27121 ggagctttag ctatagaaat tagaagcaat gatagtacga tagagggaat agtaaattca
    27181 ctgaaggatg agaatacaga aatccaaata ttggctgaaa gaggtttttt agatggtgta
    27241 aatggaagtt gtcatattcc aatggcagct tattgtgaaa taatccaaga taaaatacat
    27301 cttactggct tatatggaga ttcagaagga aaaaaagtag taatcaagtc tatagatgga
    27361 gatatttctt ctcctagaga gttaggttta aagttagcta aacttgtatt gaaggagtat
    27421 gagaattatg aagggtaagg tttacttagt aggggcagga ccaggtgatt ataaactcat
    27481 gactttaaaa ggtttagagt gtataagaaa atcggatgta atagtatatg atagattagc
    27541 aaatagtagt tatttaagag aagctaagcc tgattgtgaa cttatatatg taggcaaggc
    27601 atcaagtaat catattttgc cacaagaaga tataaataga attatagcag aaaaagctaa
    27661 agaaggaaaa attgtaactc gtcttaaagg aggagaccct tatgtatttg gcagaggggg
    27721 agaagaagca gagactttag tagatgaagg tatagaattt gaagtagttc caggtataac
    27781 ttctgctata ggaggtttat gttatgcggg tattcctata acacatagag accatgcttc
    27841 gtcatttcat gtaataacag gtcatttgaa gggtgatgat agtggagaat taaactggaa
    27901 tgctcttgca aataacaaag gtactcttgt gtttttgatg ggaatttcaa acctagagaa
    27961 aataagtgaa aacttaatga aagaaggaaa agataaggat acacctgttg ctcttataag
    28021 ttgggcaact agatataatc aaagagttgt tacatctaca cttgaaaatg tttatgaaac
    28081 tgctattaaa gaagaagtaa aaccgccaac tcttatagta gttgggagtg ttgtaggact
    28141 tagagaaaag ttgaacttct ttgaaagtaa gcctttattt ggaaaaagta ttgctgtaac
    28201 taggtctaga aatcaaaata gtgtattggt agaaaaaata atggatttag gtggaaatcc
    28261 tatagaaata cctactatta aggtagaaaa aatacaaaat aatattaact tagaaaatga
    28321 aataaaaaat ataaataagt ataattatct aatactaact agtaaaaatg ccgtagaaat
    28381 cttctttgaa aagatatatg aaatgaattt tgatttaagg atattatcta atttaaaggt
    28441 atgtgctata ggttctgcta catcaaatga gttgaaaaaa agaggaatta tagcggatat
    28501 agtaccaaaa aaatttgtag cagagtcact gtatgaagag ttagcgccta ttttaaatga
    28561 tgacgataat atacttatac ctagagcaga aaatgctaga gattatctgg ttgacaaact
    28621 caaggctatt tgtgaagtaa cagaagtaca tatatataga acagtaatag atgaggagaa
    28681 aaaaggtgag atattagata ttttaaattc taatgacata gattatataa catttactag
    28741 ttcctcaaca gtaaataatc ttgttgaaat tataggaaaa gaaaatatta aaatattaga
    28801 aaaaataaaa acactatcta taggtcctat aactacagag acaataaata gtttgggaat
    28861 aaaattgtac aaagaatctg ctacatctac tatagattcg ttagtagatt taattataaa
    28921 aaattaattt tggaggcttg aatatgttaa gaagaccaag aagactaaga gcaagtaaag
    28981 caatcagaaa tttggtaaga gaaactaagg tgaatgtaga agacttaata tatccactgt
    29041 ttgtagtaga gggggaaaat ataaaagaag aaattaaatc tttaccagat gtctatcatt
    29101 tatcaataga tatgctagaa gacgaaataa aagaaataag tgagttggga attgaacata
    29161 taatgttatt tggtgttcca gatgaaaaag atgagtgtgc ttcacaagct tttaatgata
    29221 atggaatagt tcaaaaggca attagaaagg taaaagaaat aaattcagat ataaatgtaa
    29281 taacagatgt ctgtatgtgc caatatacaa gtcatggaca ttgtgggatt ttaactgata
    29341 ctggttatgt tgataatgat aaatcattgg agtatttggc taaaatatct gtaagccatg
    29401 ctaaagcagg agctgatatg gttgctccat ctgatatgat ggatggaaga atacaagcaa
    29461 taagagaagc tttagatgaa aatggattag aaaatgttgg aatcatggct tatagtgtta
    29521 agtatgcttc aaattattat ggacctttta gagaggcagc tcattcagca ccatcttttg
    29581 gagatagaaa aacatatcaa atggacccag caaatagcaa tgaagctata atagagactg
    29641 aacttgatat attggaaggt gcagatatac ttatggttaa acctgcatta tcatatttag
    29701 atattataag aagagttaaa gataattttg acttacctct agcagcttat aatgtaagtg
    29761 gagaatactc tatgcttaag tttgctgtga aggaaggttt attaaatgaa gatgtaattc
    29821 ttgaatcaat tacatctata aaaagagcag gagcagatat aataataact tattttgcta
    29881 aagacattgc taaactgttg aaaaaataaa ttctagcagt ataaagaata tcaaaaagta
    29941 taggaattgt aattaactat tatacagtag ttttgtttag acatttattg aagaataata
    30001 gaaactaatt tgcaatttct atactttatt tatgtaacta aataaaattt ggaggtagaa
    30061 gatgctatat cctattaatt taaaattaga tgagttagat gtagttataa ttggtggtgg
    30121 agaagttgca tataggaaat gtaaaaattt tttagagttt aacaaaaatg ttactatagt
    30181 ttctaaacaa attttaaata aattttatga tttaaaagga aatataaaaa taataaaaga
    30241 tgattataaa gaaatatata taaaagatgc ttctgtaata attgctgcca caaataatag
    30301 agagttaaat atggaaatag gattatattg taaagcaaaa aataagctag tgaatgtagt
    30361 tgataatata gaaatatcga actttactgt tccatcatat ataaaaagag gagatttact
    30421 tataagtgtg tctactgggg gcaagagtcc ttcattatca tcaaagataa aaaaagaaat
    30481 tgaagagaga tatactgaag attatgagga gtatttaaat gtattaggag atataagaaa
    30541 aaaggttata aaaaaatacg aagataaaag taaaagaaaa gatgtgctta atatgttggt
    30601 aacacttgac ctagaagaac ttaagaagtt tgatatataa aaaataaaat aaaaaatgtt
    30661 aaaaaggtgt tgacggat
//
LOCUS       DAMJYR010000038        28119 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.038, whole genome shotgun sequence.
ACCESSION   DAMJYR010000038 DAMJYR010000000
VERSION     DAMJYR010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 28119)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28119)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28119
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.038"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(89..277)
                     /locus_tag="PC471_001704"
     CDS             complement(89..277)
                     /locus_tag="PC471_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HDF2788836.1"
                     /translation="MNKNNNLVIICMFIGMILGMAIGCAIGISKGNVGITMCYGLIFG
                     MIIGICIGTIIKNSNKKE"
     gene            1299..1694
                     /locus_tag="PC471_001705"
     CDS             1299..1694
                     /locus_tag="PC471_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2788837.1"
                     /translation="MNILKMKKTGIFLGGVLFGTVGVKILSSDCVKKTCTRATAGALK
                     VKDSVLETTTKIQENVEDILAEAKEINEQEDLSTSKVNVVQDTLKCEQEVLKTETIKT
                     VEDTNSNSIKCEEIKDNVIEEKNSTCTTE"
     gene            1737..3836
                     /locus_tag="PC471_001706"
     CDS             1737..3836
                     /locus_tag="PC471_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HDF2788838.1"
                     /translation="MNFYIKHEIRGRIRFDLQLGKLNNKQSDTLLYYLTSINGITKAK
                     VYDRTGDAVIYYTGEREYIIREITKFSFNNSKIENLVPEHTSRELNNYYRDKLANKLL
                     VKSISKIFFPKSLRFFLIGLHSIKYFKKGLSSLLNKKIEVSVLDATAIGISIFRKDIN
                     TAGSVMFLLGIGELLEEWTRKKSIDDLAQSMSLNIEKVWLRKGDTEILIPISEIKEGD
                     LVSVTMGNMIPLDGVIVSGETMVNQASLTGESLAVNKKEGSYIYAGTVIEQGNIVMCV
                     KEKAGTTRFQKIVTMIEESEKLKSSVESKFEHLADTLVPYSFLGSILTYAITRNPIKS
                     LSILMVDFSCALKLSIPISVLSAMRECNNNNITVKGGKFLEGVACADTIVFDKTGTLT
                     KAQPTVSDIITFQNYNKEDMLRLAACLEEHFPHSIANAVVYEAEKQGLSHKEMHTEVE
                     YIIAHGISTKVDDKKVVIGSKHFVFEDEKCTIPDGEEDKYNNLSDEYSHLFMAISGKL
                     SAVICINDPLRKEAKYVISNLRECGIKKIVMMTGDSEKTAKSIASKVGVDEYYSEVLP
                     EDKANFVKSEKLKGRKVIMIGDGINDSPAISESDVGIAMSEGAEIAREISDITISADN
                     LNNLIVLKQISNKLMKRVDLSSKFIIGFNLGLILLGVGGFVRPSTSAFLHNTSTVGIS
                     LNSMTNLLENTNTYNYH"
     gene            complement(4061..9271)
                     /locus_tag="PC471_001707"
     CDS             complement(4061..9271)
                     /locus_tag="PC471_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
                     /protein_id="HDF2788839.1"
                     /translation="MANKLYSEIVNLLEEGRDELRKYDLKEKSILLFLGASGVGKSTC
                     INYLKGCVMEEKTDEETGQIYITAKDSAVEIGNGVYSKTLCPEVVDIANRDFSLCDCP
                     GFFDNRGAEYMIAGAMLVRETISTSSKVKGMVVILDYQSLLDSRHTLLIETGKNLKDM
                     LGDYAKYKENFFFLINKIPKTLIGNVTEEKIRNILEKAKEDLDSQEKDYDGCKELVYL
                     LNVALSDGVSIKLCNPLDKQPEKLLDEMKNLPETFEKTDLGLMLTASCKIEIQKAVDV
                     TYKDLEKLLVPYIESVKKGMEDSISILSSINSLKDKKIQLDKMYTDLCSRTNNMKELM
                     EFVQELSYGDGVQKVNILEETRLLLELNSYCSETHIVGLSELLKSLLKNTLEDTLKDI
                     KYWIAEEEYREEEAKKLEDAEYRIEKIQSCTYIKSYEKENVLSIYIPIPKVNLSDLTK
                     IISGYQLNILSSIYMYGDCELKIDCNMEKEVFSGKNIVLLTEKLHIPEGTYSINVSGK
                     DAEKLVGESSIYDGMNGRNGNNAGTSGDIILSFKEIEKRGELKLYVDGGRGGNGQDGK
                     KGDTGITGEAGANAKIGSKKESKEFANVKGTKYYVVKAEKGKQGGTGGTGGNGGNAGL
                     GSKSGQVYLLGKAPLLKSAISKKDGNHGLAGKGGSGGAGGAGGLGGIRGIYEETIRYI
                     GTSPPMDNSDRNYYYFDRYVFNHALNQKQAADDGEKLNTLYEEHKDWTISKREDSGSA
                     GAEGKAGHSGDALKEKELLKQEYSLSIDTFFYEFFERFGFSGIKGYRNLRLLCAVGIL
                     EADILEFSHIYEIFYAVEKIEMQLNREILQKYYSFEKKNKAVLSMDDMNIKKEFYKEA
                     KEIYEYLINKLMQSDRIESFVGKNQNFSEWSMILFALEQKTAFYVQMIEESEIGGNRV
                     TDIRSLTDYLDKTLRELEQGKKDELKEGYFNTYKESILTQIESGNQQIEVLKNELENK
                     MLSVNAKMNLLIQEIIKMKEDEQEDIEELKKKQAKLEEQMKTQLILSGIQSALSLLTA
                     FSGVGQACLGAFDSAKSLKENLSAKEEEYQWTNRMVDFSDFKLDSDGMRFVLGEQLSE
                     DRKNALDKMTYNLAGVDEERRTKFDQIKEIIVKSIDDDNYADKADDIQRAKEGLSESE
                     YKEIYTKIQKKEIGMLEANYNNKQQKDKLAALEKSKQAIERQQKTSKIKNALSIGSSA
                     CSLINTAISGVNNCLNVKSSFESKIEVVKEAIKDKENNIKKLDELNTQMVEFKDKTLK
                     ESVIPFVEKFDSTVKNQDIFENEISKLKMKEVLTELSTKFGSLKMENSEEISIIFKKV
                     QDMMETQIIITDKIDKKKDNLAMGELIYQMSDSGNQMNEYQKKLIHQIELNQVRYLCQ
                     VEAAAFQLWTFPFGSNIVNILNRCHVGITEDENEVINQAKEQNRQIREFLDQDAYQWQ
                     KTDNHILKVALTGSLDGKSRVGFENTYSLYDCNEEEVKKLLTGDEISFEVPLNKNFDA
                     IKYVTMYANIPNLRRNILEDSLKVKVHIHLYDSGYFSLYDDKKDVYYQYVFPQNYINI
                     THGLDIKYNDSDSINSIWSSVEDKGIEKFKNAGLNSGRSPYSMAKISLKAHFNKKLSY
                     DSLTNEQKKKLESYFDKPSKYAWSDSIDILRSLGYIDLKGILSKSWYEIKKDIDSDGE
                     IMKHFSILQVDLLERIIYEQTSILKENIPNLTIELVGCGIYVESEFNYLKQNDLKCYE
                     ERLVENTIIG"
     gene            complement(9286..11781)
                     /locus_tag="PC471_001708"
     CDS             complement(9286..11781)
                     /locus_tag="PC471_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDF2788840.1"
                     /translation="MPENNNQLLEEITLLLNEGKVAINKAENQNLILILGGTGCGKST
                     AINYLAGCVMMEVEDEDTGISYIDCLDPVAEIGSGAVSQTLYPNIVNMRKPIEGMDAK
                     LCDTAGFSDNRGVAHDISAAVSLGEVFKNSASICAVIVLIPEADILDSRLTKVLDLFQ
                     QLNMCLHKENFKGSITFFISKSFLGRTEKQIYNVIKRKYEQLCENLDSNIEEKAWLFE
                     ELLKDNGKNIHLCKPLASDEREGLLKQVLELDEVKDKTSAFQYPISADARLVLDTLIK
                     EIQNNIVSSLEDYVKAYTEDFINIIGSITMIQEIAKYDEVVKVFKNWLESTTSLTIES
                     FINVLAKQSGLFLQDSCMKLQNKIKGNLDIIDALSKYTEHGTGEILLPSVHMEFIIEK
                     ASKKIMEQNFIIIRKNLEKVLVSYEVQKQVFTLPNREKLMEVANKKDYVYSVSDFNSI
                     LTNVTVTQNEELEDSITRLSAYGEEGNEIIREFIGKYILNPFIVKTININDKKGIEIR
                     ALIPNLVISSVLEYFKNRKVDDNVSTFMFFAKDTIYMDDNLGMNFASEKNIILASNNL
                     DVGSNVKINVSGKSGELVDNSMYGKNGEDGRDAGNIYLYIKEEIRNYRLTLIANGGNG
                     SKGIDGENGETGASGSNGADAGYTSYFHGQMFKGYFEKGTSGKKGEKGGAGGNAGRGG
                     NAGKKGNIYFISDSEEQKNRIINHCDITVKDGEAGPNGRPGLGGYGGSGGYHGYNTLS
                     FSPSGIDKTVYYTGYFTSWDYKLDSIIKYHIEFSGGSWNEGKTGPAREYAEYGDRGDN
                     GGFNAPNTKLSTAVSKVEENLIKEKYQGYCSEI"
     gene            complement(12442..12729)
                     /locus_tag="PC471_001709"
     CDS             complement(12442..12729)
                     /locus_tag="PC471_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2788841.1"
                     /translation="MRLNITKQNTIEVENSRKEDVYFVIVDNVFFERQGDVFNSITNN
                     NLNNYDVKILPTDNIFLELKITSTKDLEPVFIVVEDRQYNHGSIMFKPVLV"
     gene            complement(12749..13024)
                     /locus_tag="PC471_001710"
     CDS             complement(12749..13024)
                     /locus_tag="PC471_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2788842.1"
                     /translation="MSLFVGGWSKFRAVEEEDLKVFNEAVGMLEGVGYEPLIVSTQVV
                     AGTNFKFICNATSVTNPPQNYLAEIVVFKPLPCDEHNATITSINIIK"
     gene            complement(13679..13858)
                     /locus_tag="PC471_001711"
     CDS             complement(13679..13858)
                     /locus_tag="PC471_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2788843.1"
                     /translation="MGKDRFEKVYSQGKINATEIWVDKETGVNYVFHYYGNAAGFTPL
                     LDREGKPVISPIVSK"
     gene            complement(14143..15618)
                     /locus_tag="PC471_001712"
     CDS             complement(14143..15618)
                     /locus_tag="PC471_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDF2788844.1"
                     /translation="MKRKPQYLIIFEHIIQQIYTGKITIGDRLDSIKSMSEEYMVSKN
                     TIKTVIKMLSEKGFIETKAGSRPVLIQSTSKQSIESDLLYEKILQISEIYKIFSLIFP
                     SIAMYNIKRFTSKDFEELNEIIDCMEKNSSNYLVFKEQRLKYINKLISKTNNFLIHHF
                     CEIIQHDVIISQLAYNFDNLDNLDNCCIDFFRNKYIEQVRKTYLYGLDGNLIEFKKLL
                     TYMYKACRESILKMVENRAPQSIDTSSFNSVTLEKCYLYDLIVSDVICQIFEGNIKIG
                     DCLPSITSIINRYNVSLPTVRSAYNELNELGIAKTINGKGTYVTLFSEYDDDYINTEE
                     GAKRLSLLLDAMEFVTITLEDVVLLLGNKMDLIAVDKIETHLRYLQNNCEECKVYPDF
                     VLLCKIVDYTQIYVLQETFLHLKQYMIFGIYLERFFHEEYNEILKTRFDVCFEILRYL
                     KKGDVERFAESFSNIFKESLQYLKKVISSLNVDMRLKLGIL"
     gene            16649..17827
                     /locus_tag="PC471_001713"
     CDS             16649..17827
                     /locus_tag="PC471_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HDF2788845.1"
                     /translation="MKYHIFLIITTILIIELCNIYICKIASFGNKNITIHKNYKYIFL
                     IKPLTFLIIIFTKLPLPLYYILFYIVSHKLLLSRKSSTNNHFIPNMAVISFCILHLIT
                     VSTISLVKNTSPDIIFANMTYKLISILIASILDFILWYSMNKIKEHIYIITQNQIEFS
                     LFLKFIWFTLGYILFDSIQITFAPPSSLTSYFILGSNILLILQLIIFINHIYSIEKNI
                     YLEKEHNRLKMIKQEQLLKVNLLKKKANIDELTSAYNRKFAFEKIQHYIDNNLLFSLI
                     YIDLDKLKTINDTYGHLAGDNYIINFSSIVIENLGIDDIFARIGGDEFIVIMPKSNEN
                     TTKEFIEKISKIMDNTISFSYGIVEVSSENKMTVEEIINIADIRMYTNKQNNHKRNNN
                     "
     gene            18177..19058
                     /locus_tag="PC471_001714"
     CDS             18177..19058
                     /locus_tag="PC471_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HDF2788846.1"
                     /translation="MPAYLFDSNSIANIYRVFPISFSFIVTGLYFSYKNNKNNKINFE
                     AVLEDKKNLIFLLSLMFGMYLYLCLSLSIYYTVGNVLILKLYGIKSAIIVLVGNYIAE
                     RYSFRITQIKGYDIKNQIEYVESVKTEYEINKLNDIVYTDLLTGFYNRPFVNQKLSEW
                     TSNHYKFTLCFTDLNSLKPVNDKFGHQFGDKYISTTAQIIKKLINVENSLLFRYGGDE
                     FLFLLQNTSCVEAEKIMININYELSKLSNTDEYPYALSISYGLVEWDGISDIETLISK
                     ADSLMYENKLKNKSTLN"
     gene            19043..19416
                     /locus_tag="PC471_001715"
                     /pseudo
     CDS             19043..19416
                     /locus_tag="PC471_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418025.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
     gene            complement(19676..22243)
                     /locus_tag="PC471_001716"
     CDS             complement(19676..22243)
                     /locus_tag="PC471_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002602373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HDF2788847.1"
                     /translation="MIYYVLPLEHKQATIEIVGGKGMSLSKLLTAGIPVPDGFHVTTA
                     SYQIFVETNHIQSRINKLLDGIDSNNTSQLEDVSKKIGELFHNGEMPQEVSDAIKMAY
                     AGLGNISVAVRSSATAEDLPDASFAGQQETYLNIQGEDKVIDSVKRCWASLWTARAIA
                     YRVKNNIKHEIVALAVVVQKLAFSDSSGIMFTLNPINGRRSEMIINAAWGLGEAVVSS
                     LVTPDTIVVDKDSERIISYEVANKEIMTVRTSEGTEETMVPERLRKKYALTRNQVMQL
                     IQLGKKIEKYYQMPMDVEWALEKDKLYIVQARPITVLPPEWVLPEQDVVYTKGSLAEH
                     LPNPVTPLFATLGLEIVNRASELLWIDMFGKSAKKLLPKNGAYTVINGYVYLSAKSKP
                     LLIAIKSLSPRSLRRTLMNSVARWETARKGYEDAIKQWEEKPLHTMNAHQIMEGIQTI
                     FYAACIYFTRIQLTLPAASISETLFTKLFQGAVRRAGITDTSVFLLGFDTIVLQSEKE
                     LWGISEWVKQNNSINLYLQNSPAIKIAEDFMSSVVPVGVSQEVWIEWKNRINKYFKEF
                     GRTAYEFDFAHSTPQETLTPTFESVKTFVEGKGESPFLRQTTFEKRRKHAEEEILQHI
                     GGLRKKLFFKLLHWAQETSPMRENAIYLMGMGHPLIRCMFQEISERFIRGGAISHLDD
                     IYWLTKSELEVLIEQLDKNKSLSDMNGIIPVRKAELKKYMGYMSPSKLPEKNKKTISQ
                     AQQKQKDGKIVLTGIGTSTGVVTAPACVLNSPADFESFQPGSVLVAVTTTPAWTPLFS
                     SASAIVTDIGGPLSHSSIVAREYGIPAVMATHIATRTIKSGQMITVDGLAGTVTFDE"
     gene            complement(22246..22851)
                     /locus_tag="PC471_001717"
     CDS             complement(22246..22851)
                     /locus_tag="PC471_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002591427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDF2788848.1"
                     /translation="MPKGIVLTPEQQAERREKIVAVALSLMAENGFQKTSMREIAVLA
                     NMGKSSLYDFFKTKDEIVVYAVEKEIEKTIQQVHRIIDGEPSPQQCLRKIMLNHLRFP
                     EQYRTVLMWLNTESDYLEEEYRKRLKDARYAYQDIIKSVIDNGVTTGIFRKTDTALVT
                     RLLINSMLSISYTSRPLDSSEKMLDETMNIFLNGIMNDGGE"
     gene            complement(23225..24685)
                     /locus_tag="PC471_001718"
     CDS             complement(23225..24685)
                     /locus_tag="PC471_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent glyceraldehyde-3-phosphate
                     dehydrogenase"
                     /protein_id="HDF2788849.1"
                     /translation="MFNELKTKENVYKNLINGKWVESNSRKPIEIYSPIDNSLVGKVQ
                     SMTKHEVDEVIKNTKESIKVWAEMPVYKRGNIFHKAADLLLENIDEIANILVLEIAKD
                     IKSARAEVERTADFLRYTADVGKNMEGEAISGDNFPGGTRNKMSYVSRVPLGTVLAIS
                     PFNYPVNLSMSKIAPALIGGNAVVLKPATQGAISALHVVEIMRKAGIPDGVLNTITGR
                     GSEIGDYVVTHKGINFINFTGSTEVGQHISKISGMVPLLLELGGKDAAIVLEDADLDF
                     AAKNIVSGAYSYSGQRCTAVKRILVQESVADKLVGKIKPLVEKLTIGNPMDEVVITPL
                     IDNKATDFVQGLVDDALHKGAKLITGNVRKNNLFYPTLLDNVNVDMKIAWEEPFGPVL
                     PIIRVKDINQAIEIANQSEYGLQSSVFTSDIDKAFYIADKLEVGTVQINNKTERGPDH
                     FPFLGVKASGMGTQGVKYSIEAMTRPKAVVVNVREL"
     gene            25031..25609
                     /locus_tag="PC471_001719"
     CDS             25031..25609
                     /locus_tag="PC471_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDF2788850.1"
                     /translation="MAKSFTEIEKDNIRKRLISECEINWSKYGYKKTNIDELCSKSGI
                     AKGSFYTFFDSKEKLFFEVLNGIQNRLISSVDEILSNMPPKEGFAKSLKMLYRLYDKN
                     PFLYSPNNPDFISLINKLPQDMFLQLESNNIQSFYDLIERHNLKLKIEKEKVFGVCNA
                     LLSSLLVKENIEYNYIEVFDFMVDNLVEHILE"
     gene            25900..27150
                     /locus_tag="PC471_001720"
     CDS             25900..27150
                     /locus_tag="PC471_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDF2788851.1"
                     /translation="MVKSSNTFNKNFNLMILGQIISLFGASILKFALSLYILDITGKA
                     EIFATILAVSSIPIIVFSPIGGAIADIFNRRNLMVIFDFSSSLTVLILAVFLFNNNGS
                     ILLVGIIMTILSVISTMYQPTVQSSTPLLVDNEHLMNANGIVAGVASLTNIAGPVLGG
                     VLYGVIGINAIIVISCVSFFLSAIMEIFIQIPFIKQEQNSNIIKTIFTDIKDGIVHIC
                     KENRFILKILFLAIAINLFLSSMLIVGIPYIVKITMGASNFLYGLAEGAISFSSLVAA
                     VSIGIFSKHLKITNLHISFIICAVMFIPMAVSVYPFILNTGFPVPFLIFIVSSMLIFF
                     TTTLISIYVITVTQLKTPNELLGKVMSILFAGTAIAMPLGQVMYGKLFDLLSDRVYII
                     ILGVGFITLLIAFVSKITLRNENL"
     gene            27311..27880
                     /locus_tag="PC471_001721"
     CDS             27311..27880
                     /locus_tag="PC471_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HDF2788852.1"
                     /translation="MSYMPTLEQAWEILRKYNKEEFHIRHAQIVSGVMRYYAKEYDPE
                     RVEFWEIVGLLHDLDFEMYPDEHCVKQRELMTELDLDKSIIDSTISHGYGLTGSDVKP
                     EQFMEKVLFAVDELTGLIGAAAIMRPSKSVSDLELKSVKKKFKDKSFAAGCSRDVIRS
                     GAEMLGWELDKLIQTTIEAMRSLIPEMAI"
BASE COUNT         9515 a         4785 c         3429 g        10390 t
ORIGIN      
        1 agtatgtctt attctacttt ttacagataa ttaaaaagtt ttatcataaa taaatcataa
       61 ctagacatcc tagtaaatca aaattaattt attctttttt attcgaattt ttgataatag
      121 tccctataca tatgccaatt atcattccaa aaattagacc ataacacatt gtaataccta
      181 catttccttt agaaatacct atagcacaac ctatagccat tccaagtatc atgcctataa
      241 acatacatat aataactaaa ttgttgtttt tattcatagt ttttctccat tcaatatatt
      301 ttaaattgcc tagctcgcct tcttagctta aaacaaagaa atataaatta actttctttc
      361 attctaatat ctgcctgtat aaatgtcaaa caacacattt cttgtcataa cctcctcttt
      421 ttgaagtaag tacaatctcc cagacgcaat aatatcaatt tcctaaaacc atttaagtaa
      481 cttttatatt tacataatat ttacaatcat aaactatatt gttattcatt tatacatcat
      541 aaacactccc tcctaatcat tattatactt aaaataatgt tcatttataa taatatatct
      601 aacgcacttt aattgaaaaa actaaaactt tgcctttcat tcccatatat aaataagtat
      661 actttttgat gctctattac aaatcaacta aattaattga tataaattat cattttctgt
      721 tgaaaatacc attcgcacat gctatactta attgcataaa tggtaataaa tgtcatatga
      781 ttttacaaat ctattaataa caattattac ttttactgta ttttaaagtt tttggactaa
      841 atatcaagaa ctttctaaaa tacaaagtac aaaaaattta tataagttat aaatttatat
      901 gaactagata agctcaaaca atatgttatg tataaactgt gtagtattcg gttataaatt
      961 tatataaatt atataagcta gataaagtat tacctcaact atataagcta gatatataaa
     1021 ttgcttatat aagctagata agaattattt caactatata aactacatat ataaattaca
     1081 tacataagct agatatataa actgcatata taagctatat aagaattatt tcaaccatat
     1141 aaactacaca tgtaaaattc atataaattt actaatataa atcaaataaa attactaaac
     1201 tctctaaact aattttgtaa aacatactct atttttaaca tttgttacct tatacataag
     1261 tacaaacaaa atctaactaa atgaaaggat aatgtaaaat gaatatttta aaaatgaaaa
     1321 aaacagggat atttttagga ggagtacttt ttggtactgt aggtgttaag atactttcaa
     1381 gtgattgtgt taaaaaaact tgcactcgtg caacagcagg agcattgaaa gttaaagatt
     1441 ctgttcttga aacaactacg aaaattcaag aaaatgtaga agatatttta gctgaagcaa
     1501 aagaaatcaa cgagcaagaa gacttatcta catctaaggt aaacgttgta caagatactc
     1561 ttaaatgcga acaagaagtt cttaaaactg aaactataaa aactgtagaa gatactaatt
     1621 ctaatagcat taaatgtgaa gaaatcaaag ataacgtaat tgaagaaaaa aatagtactt
     1681 gtacaacaga ataaatttaa tgattattat gattaataat atcaggagga agtaaattga
     1741 atttttatat aaaacacgaa ataagaggtc gtatacgctt tgacttacaa cttggcaaat
     1801 taaacaacaa gcagtccgat actctcttat attatttaac ttctataaat ggtattacca
     1861 aagcaaaagt ttacgataga actggtgatg cagtcattta ttacactggt gaaagagaat
     1921 acataataag agaaatcact aaattcagtt ttaacaatag caaaatagaa aacctagttc
     1981 ctgaacatac cagcagggaa ttaaacaatt attatagaga caaattagca aataaactgc
     2041 tagtcaaaag tatctccaaa atattttttc caaagtcctt aagatttttt ttaattggac
     2101 ttcattctat aaaatatttt aaaaaaggtc tctcttccct gctaaataaa aaaatagaag
     2161 tttctgtttt agatgccact gctataggta tttctatttt tagaaaagat atcaatacgg
     2221 ctggttcagt tatgtttctt cttggtatcg gtgaactatt agaagaatgg actagaaaaa
     2281 aatctataga cgacttggct caaagtatgt cactaaacat tgaaaaagta tggttaagaa
     2341 aaggtgacac tgaaatctta attccaattt cagagataaa ggaaggagat ttagtaagtg
     2401 taactatggg aaatatgatt ccattggatg gagttatagt atcaggagaa acaatggtca
     2461 atcaggcatc acttacaggt gaatcccttg ccgttaacaa aaaagaaggc tcttacatat
     2521 atgctggaac tgtaatagag caaggcaata ttgttatgtg cgtcaaggaa aaagctggta
     2581 ctactagatt ccagaaaata gtaactatga ttgaggaatc tgaaaaacta aaatcttctg
     2641 tagagagtaa atttgaacat ctagcagata cattagttcc atacagcttt ttaggaagta
     2701 tattaaccta tgcaattact agaaacccta taaaatcact ctctatatta atggtagatt
     2761 tctcttgtgc attaaaactc tctatcccta tttcagtact atcagccatg agagaatgta
     2821 acaataacaa cattactgta aaaggtggaa aattccttga aggtgttgca tgtgcagata
     2881 ctattgtatt tgataagaca ggcacattaa caaaagctca accaactgtt tcagatataa
     2941 ttacattcca aaattataat aaggaggata tgttaagact tgcagcttgc ttagaagaac
     3001 actttccaca ctccatagca aatgcagtag tttacgaagc tgaaaaacaa gggcttagtc
     3061 acaaagaaat gcatacagag gtagaatata ttattgctca tggcatttca acaaaagttg
     3121 atgataaaaa ggttgtaata ggtagcaaac attttgtatt tgaagatgag aagtgtacta
     3181 tcccagacgg tgaggaggat aagtacaaca atctatcaga tgagtattca cacctattta
     3241 tggcaatatc aggtaaatta tcagcagtta tttgcataaa tgacccttta cgaaaagaag
     3301 caaaatatgt aatttcaaat ttaagagaat gcggaataaa aaagattgtg atgatgactg
     3361 gagatagcga aaaaacagct aaatctatag caagtaaagt tggtgttgat gaatattatt
     3421 cagaagtctt accagaagac aaagccaatt ttgttaaaag tgaaaaactc aaaggtcgca
     3481 aagtaattat gattggtgat ggaatcaatg attctcctgc tatttcagaa tcagatgtcg
     3541 gaattgcaat gagtgaaggt gctgaaatag caagagagat ttcagatatt accatatcag
     3601 cagataattt gaataattta atcgttttaa agcagattag taataagctt atgaaaagag
     3661 ttgatttaag ttctaagttt attattggct ttaatttagg tctgatttta cttggtgtag
     3721 gtggatttgt tagaccaagt acatctgcct ttttacacaa cacatctact gtcggaataa
     3781 gtttaaacag tatgacaaat ttattggaaa acaccaatac ttataactac cattaatctc
     3841 cattacataa aacatatgat ataaaaaatt tataattcat ctttttaaaa gaatatgtct
     3901 taaaattagc ttttttctaa tcaaaattat ataaagcata ctttacaaat tttaaactca
     3961 acttatttct attttttaca aaaaaggaga tgattcaaat tttcattttg aaacatcccc
     4021 ttttgtaaat ttctttaaaa tttatatatt aattatattt tcaaccaata attgtatttt
     4081 caaccaatct ttcttcatag cattttaaat cattttgctt tagataatta aattcagact
     4141 ctacataaat accacagcct accaattcaa ttgtcaaatt aggtatattt tcctttagta
     4201 tactagtctg ctcataaata attctttcca ataaatcaac ttgtaatata gaaaaatgct
     4261 tcataatctc cccatcacta tctatatctt tcttgatttc ataccaactc tttgacaaaa
     4321 ttcccttcaa atcaatatac cctagactcc tcaatatatc tatactatcg ctccatgcat
     4381 acttactagg tttatcaaag tatgattcta gtttcttctt ctgctcattt gtaagtgaat
     4441 cataagaaag cttcttatta aaatgtgcct tcaaagaaat cttagccata gaatatggac
     4501 ttctaccaga gtttaatcct gcatttttga acttttcaat tcctttatct tctactgaag
     4561 accatattga gtttattgaa tctgaatcat tgtatttaat atcaagacca tgtgtaatat
     4621 ttatatagtt ttgtggaaat acatattgat aatagacatc ttttttatca tcatatagag
     4681 agaagtatcc actatcatat aaatgaatat gcacctttac ttttaaacta tcctctaaaa
     4741 tatttcgtct aaggttcggg atatttgcat acatggttac atattttatg gcatcaaagt
     4801 ttttatttaa tggaacttca aaactaattt catctcctgt caaaagcttt ttcacttctt
     4861 cttcattaca atcatataga gaatatgtat tttcaaatcc cacacgtgat ttcccatcta
     4921 aactgcctgt taatgctacc ttcaaaatat ggttatctgt tttttgccat tggtaagcat
     4981 cttggtcaag aaactctcta atctgtctat tttgctcttt cgcctgattt ataacctcgt
     5041 tttcatcttc tgtaatccct acatggcatc tatttaagat atttactata ttgcttccaa
     5101 atgggaatgt ccatagttga aaagctgctg cctcaacctg acacaaatat ctaacctgat
     5161 ttaattcaat ttgatggata agttttttct gatattcatt catctgatta ccactatctg
     5221 acatttgata aatcaattct cccattgcaa gattatcttt tttcttatca atcttatcag
     5281 taatgataat ttgagtttcc atcatatcct gtactttttt aaatattata gaaatctcct
     5341 ctgagttttc catttttaag cttccaaact tcgtgcttaa ttcagttaaa acttccttca
     5401 tctttaattt acttatctca ttttcaaaaa tatcttgatt tttaactgta ctatcgaatt
     5461 tttctacaaa tggtataaca gattctttca aagttttatc tttaaattcc accatttgag
     5521 tattcaattc atctaatttc ttaatattat tttctttatc tttaattgcc tctttaacta
     5581 cctctatttt actctcaaaa ctagatttta cattaagaca attattaaca ccactaatcg
     5641 cagtatttat aagagaacaa gcagaacttc caatactcaa tgcattttta attttagatg
     5701 tcttttgctg tctttcaata gcttgtttac ttttctctag tgcagctaac ttatcttttt
     5761 gctgtttatt attgtaattt gcctccaaca tacctatttc ttttttctgg atttttgtgt
     5821 aaatttcctt atactcgctt tcacttaacc cctcttttgc tctttgaata tcgtcagctt
     5881 tgtctgcata gttatcatca tctatagact taactataat ctccttaatt tggtcaaatt
     5941 tagttcttct ttcttcatca acacctgcca aattataagt cattttatct aaagcatttt
     6001 ttctatcttc acttagctgt tctccaagta caaatctcat accatcagaa tccaatttaa
     6061 aatctgaaaa atcaaccatc ctgttagtcc attggtattc ctcttcttta gctgaaagat
     6121 tttccttcaa agattttgca ctgtcaaatg cccctagaca agcttgacca acaccactaa
     6181 atgcagtcag taaactcaat gcgctttgta ttccagaaag aattaactgt gttttcattt
     6241 gttcctcaag ttttgcctgt ttctttttta gttcctctat gtcttcttgt tcatcttctt
     6301 tcatctttat aatttcttga atcaacaggt tcatttttgc attaacagaa agcattttat
     6361 tctcaagttc attttttaaa acctctattt gttgatttcc agattcaatt tgagttagga
     6421 tactttcctt atatgtattg aaatatcctt cttttaactc atctttcttt ccttgttcca
     6481 attctcttaa ggtcttatcc agatagtctg tcaaagaacg aatatctgta acacgatttc
     6541 ctccaatttc actttcttca atcatctgca cataaaatgc tgtcttctgc tcaagtgcaa
     6601 ataaaatcat actccattcg ctaaaattct gattttttcc tacaaaagat tctattctat
     6661 cagactgcat caatttattt ataaggtatt catagatttc ctttgcctct ttataaaatt
     6721 ctttctttat attcatgtca tccatagaaa gtactgcttt atttttcttc tcaaaactat
     6781 agtacttctg aagtatttct ctatttaatt gcatctctat tttctcaact gcataaaaaa
     6841 tttcataaat atgtgaaaat tctaaaatat cagcttctaa aattcctact gcacacaaca
     6901 gtctaagatt tctataacct ttaattccag aaaaaccaaa tctctcgaaa aattcataga
     6961 agaatgtatc tattgacaaa gaatactcct gctttaacaa ttccttttct tttagtgcat
     7021 ctccagagtg accggctttt ccctctgctc cggctgaacc tgaatcttca cgtttgctaa
     7081 ttgtccagtc cttatgttct tcataaagag tatttaattt ctctccatca tctgctgctt
     7141 gcttctgatt taaagcatga ttaaacacat atctatcaaa ataatagtag tttctatcac
     7201 tattatccat tggaggagag gtacctatat atcttattgt ttcttcatat atacctctaa
     7261 ttccaccaag acctcctgca ccaccagctc ctccagagcc tccttttcct gctaaaccat
     7321 gatttccatc ttttttactt atagcacttt ttaataaagg agcttttcca aggagataaa
     7381 cctgtccaga cttgcttcca agacctgcat ttccaccatt acctcctgtg cctccagttc
     7441 caccttgttt tcctttttct gcttttacaa catagtattt tgtaccttta acattagcaa
     7501 attcttttga ctctttttta gaacctattt tagcatttgc cccagcttct ccagttatcc
     7561 ctgtatctcc tttttttcca tcttgtccat ttccaccacg tccaccatct acgtaaagtt
     7621 taagttctcc tcttttttct atttctttaa aagataatat gatatcacct gaagtacctg
     7681 cattattgcc atttcttcca ttcattccgt cgtaaataga actttctcca accaattttt
     7741 ctgcatcctt accagatacg ttgattgaat aagtcccttc tggtatatgt aatttttctg
     7801 taagtagtac aatattctta cctgagaaga cttctttctc catattacag tcaattttca
     7861 actcacaatc tccatacata tatatagatg acaaaatatt aagttgataa ccagatatta
     7921 ttttagtcaa atccgataaa ttcactttag gtataggaat atatatagaa agtacatttt
     7981 ctttctcata tgattttata tatgtacaac tttgaatctt ctctatccta tactctgcat
     8041 cttcaagctt tttggcttct tcttccctat attcttcttc tgctatccaa tatttgatat
     8101 cttttaatgt atcttctaaa gtatttttta gcaatgattt taacaactca cttagaccaa
     8161 caatatgagt ctcactacag taactattta actctaaaag aagacgtgtt tcttctaata
     8221 tattcacttt ctgtacgcca tcaccatatg aaagttcttg tacaaactcc ataagctctt
     8281 tcatattatt agtcctacta cataaatctg tatacatctt atctaattga atctttttat
     8341 cttttagact atttatagaa gatagaatgg aaatagaatc ttccatacct tttttaacac
     8401 tttctatata aggaactaaa agtttttcta ggtctttata ggttacatca acagcttttt
     8461 gtatttctat tttacaagaa gctgtaagca tcaatcccaa atctgttttt tcaaatgttt
     8521 caggtagatt tttcatttca tccaacaact tttctggctg tttatctagt ggattacata
     8581 atttaataga tacaccatct gataatgcta catttaataa gtacactaat tccttacaac
     8641 cgtcgtaatc tttctcttga ctatctaagt cttcttttgc tttttccaat atatttctta
     8701 ttttttcttc tgttacatta ccgattagtg ttttaggtat tttattaata agaaagaaga
     8761 aattttcttt atactttgca taatcaccaa gcatatcttt caaatttttg cctgtttcta
     8821 tcaataatgt atgtctagag tccagtaggg attgatagtc taagataact accatccctt
     8881 tcacctttga agatgtggaa atagtttccc ttacaagcat agcaccagca atcatatact
     8941 ctgctccacg gttgtcaaaa aaacctgggc aatcacataa tgaaaaatct ctatttgcaa
     9001 tatctacaac ttctggacat aaagtcttag aatatacacc atttccaatt tcgacagctg
     9061 aatcttttgc agttatatat atttgtccag tctcttcatc cgttttttct tccatcacac
     9121 agccttttaa ataatttata caagtactct ttccaacgcc tgaagcacct aggaataaca
     9181 aaattgactt ttcttttaag tcatattttc taagctcgtc tcttccttct tccaaaaggt
     9241 ttacgatttc actataaagt ttatttgcca tacatccatc cctttctata tttcggaaca
     9301 atagccttga tatttttctt taataagatt ctcttctacc tttgaaacag ctgttgataa
     9361 cttagtattt ggagcattaa atcctccatt gtctcctcta tctccatatt cagcgtattc
     9421 tcttgctggt cctgttttac cttcattcca gcttccacca ctaaattcta tatgatattt
     9481 aattatacta tctaacttat aatcccaact tgtaaaatat cctgtatagt atacagtctt
     9541 atctatacca cttggagaaa atgaaagtgt attatatccg tgatagccac cacttcctcc
     9601 atatccacct aaacctggtc taccatttgg acctgcttct ccatctttca ctgttatatc
     9661 acaatgatta attattctat ttttttgttc ctctgaatct gaaatgaagt aaatattacc
     9721 tttcttacca gcatttccac ctcttccagc atttccacca gctccacctt tttcaccttt
     9781 tttgccactt gtaccttttt caaaataccc tttaaacatc tgtccgtgga aataacttgt
     9841 atatcctgca tcagctccat tacttccaga agctcctgtt tcaccatttt ctccatctat
     9901 tcctttagaa ccatttcctc catttgcaat cagtgttaga cgataatttc taatttcttc
     9961 tttaatatat aagtaaatat tacctgcatc tctaccatct tcaccatttt tcccatacat
    10021 agaattatct acaagttctc cgctctttcc acttacatta atttttacat tactacctac
    10081 atcaagattg ttagaagcaa gaataatatt tttctctgat gcaaagttca ttccaaggtt
    10141 atcatccata taaatagtat ctttagcaaa gaacataaaa gtagaaacat tgtcatctac
    10201 ttttctattt ttgaaatatt ccaatacact gctaattact aagtttggaa ttaatgctct
    10261 tatttcaata cctttcttat cattaatatt aattgttttt actataaatg gatttaaaat
    10321 gtatttacca ataaattctc taattatctc attaccttct tctccatatg cacttaatct
    10381 tgttatactg tcttctaact cttcattttg tgtaactgta acatttgtca aaattgaatt
    10441 aaaatcactt acactatata cataatcctt tttatttgca acttccatta atttttctct
    10501 atttggtaat gtaaatacct gtttttgcac ttcataagaa actaaaactt tttctaaatt
    10561 ctttcttatt ataatgaagt tttgttccat tatcttctta cttgcctttt ctataataaa
    10621 ttccatatgt acagaaggta ataaaatttc cccagttcca tgttctgtat atttacttaa
    10681 agcatcaatt atatctaagt ttccttttat cttattttgt aatttcatgc aactatcttg
    10741 taagaataat ccactttgtt tggctaatac gttgataaaa ctctctatag taagacttgt
    10801 tgtactttcc aaccagtttt taaatacttt tacaacctca tcatattttg ctatttcttg
    10861 aatcattgtt atacttccaa taatattgat aaaatcttct gtatacgcct taacataatc
    10921 ttctaggcta gatacgatat tgttttgaat ttcttttatc aacgtatcta aaactaatct
    10981 tgcatctgca ctaattggat attggaatgc tgatgtctta tctttaactt catctaattc
    11041 tagtacttgc tttaataagc cttccctctc atctgatgca agtggcttac aaagatgaat
    11101 attttttcca ttatctttta gcaattcttc aaataaccaa gctttttcct ctatattact
    11161 gtctaaattt tcgcataatt gttcatattt tctttttata acattatata tttgtttttc
    11221 tgttcttcct aagaaacttt ttgaaatgaa aaaagttata ctacccttaa aattttcttt
    11281 atgaagacac atatttaact gttggaacag gtctagtact tttgtaagtc tagaatctaa
    11341 tatatctgct tctggtatca atacaattac tgcacatata gatgcagaat ttttaaatac
    11401 ttcacctaat gaaactgctg cactaatatc atgggctact cccctattgt cgctaaaacc
    11461 tgcagtatca cataattttg catccattcc ttctattggt tttctcatat ttacaatgtt
    11521 cgggtataaa gtttgagaaa cagctcctga accaatctct gccacagggt ctaagcaatc
    11581 aatatatgat ataccagtat cttcatcttc tacttccatc attacacatc ctgcaagata
    11641 gttaattgca gtacttttgc cacatcctgt cccaccaaga attagaatta aattttgatt
    11701 ttcagcctta tttattgcta cttttccttc atttaataaa agtgttattt cttctaaaag
    11761 ttgattgtta ttttctggca tttattttcc cccttggtta tatattttat ttaaataaag
    11821 tttaatccct taagtagatt gcaaagtttt tagactatat ttaaatttgt tttttatttt
    11881 ttatttttaa taaatttgca ttttcactaa ataattattc ttctttgttt attaaaataa
    11941 ctgtgttgta taatataatc tttatcaaca cagttatttt atttaatgcg cttattttat
    12001 tgtattttat gtaatacagt tgttttttat attatatgtc cattattttt ctatggttat
    12061 tacatataga tttttaatta tatttgtacc attaatttaa acattcctat actactgctt
    12121 aagttttaca cctataccag cttgaatatt ttatttttat ttgcttaaat agttcaacta
    12181 tactggttta aataggttat ctttatttgc ttaaatagtt caactatact ggttaaatag
    12241 gttatcttta tttgcttaaa tagttcaact atactgattt aaataggtta tctttatttg
    12301 cttaaataag ttcaactata ctgatttaaa taggtttcct ttagtttaaa taattcacct
    12361 atactggttt aaataggttt cctttagttt aaataattca cctataccta cttgaatagt
    12421 tcacttttat ttgcttaaat tctataccaa tactggctta aacattatac taccatgatt
    12481 gtattgtcta tcttcaacta caataaaaac aggttctaaa tctttagtag atgttatttt
    12541 taattctaaa aaaatattat cagttggtaa tatttttaca tcataattat ttaaattatt
    12601 attcgtgata ctattaaata catcaccttg acgttcaaaa aaaacattat caactattac
    12661 aaaataaaca tcttcttttc tactattttc aacctctata gtattttgtt tagttatatt
    12721 tagtctcatc ttccacctcc tattaatatt acttaattat attaatactt gtaattgttg
    12781 cattatgttc atcacaagga agaggtttaa aaacaactat ctctgctaaa taattctgtg
    12841 gaggatttgt aactgaagta gcattgcaaa taaatttaaa atttgttcct gctacaactt
    12901 gagtagaaac aataagtggt tcataaccaa ccccttctaa cattcctaca gcttcattaa
    12961 atacttttaa atcttcttct tcgacagctc tgaattttga ccatccacca acaaaaagac
    13021 tcatataaaa acctccttta tatatatatt tatttatata tagtagtacc aattttctca
    13081 atattcaata ttttctgtgt aactggtcaa tgaaaatgca taactggata aaaatactta
    13141 taaaataact taactttaca tataaatagc ttactttaca tgtaataaga ttattctaca
    13201 tgtaataaga ttattctaca atatgaaatg catcttgttt tgaatatatt ctaaatacaa
    13261 taatggatat tttcaattat tataattaga aaatatccat tacttaactt gttttattta
    13321 aaatcacata gagactttat tatttttttt attgcatgaa tagctatttc aggtatcata
    13381 aggtgatata ccccctcctt gaaaccacaa aaaacatgac aaactttacg aatcttgtct
    13441 taaaaaacta caggtacatt ccttatttgt ggattggagt agccataaca agcaaatttt
    13501 acaattttta tgatgacaaa aatcaataaa atcgcttagt atataacacg aaatactact
    13561 tgttactatt aagaatagtt agataggaag tatgcattat acaaataaag gaatcaataa
    13621 agaaagttaa aacatcccta caaattaaaa atctgttaca tttttctata tttaaacgtt
    13681 atttacttac aattggagaa ataacaggtt ttccttctcg gtcaagtaaa ggcgtaaatc
    13741 cggctgcatt gccataatag tggaatacat agttcacacc tgtttcttta tccacccaaa
    13801 tctccgttgc atttattttc ccttgtgaat atactttttc aaatctatct tttcccattt
    13861 tataatcctc catatttttg attggtctat gccttcttca cttaagtaga aaaaatacat
    13921 aacttatttt tttaattcta atatgagcac gtaaaaatgt caaacagctt atttaatttc
    13981 ataacttcat gttactgttc gtaaccttgt ctgtttactt acactgcatt acagcaccat
    14041 tttatccttt acattattgt attgcacaca gtaaaaatgc ttactttatg ggatttttca
    14101 actaaaaaaa ggattttatc tccacaaaac atttactatt tgttacaata tacctaattt
    14161 taatctcata tcaacattta atgaagatat aacctttttc aaatattgta aagactcctt
    14221 aaaaatatta gaaaaagatt ctgcaaatct ctcaacatct ccttttttta aatacctaag
    14281 tatctcaaaa catacatcaa aacgtgtttt caaaatttca ttatattctt catgaaaaaa
    14341 tctctctaaa tagattccaa atatcatata ttgttttaaa tgcaaaaatg tttcctgtaa
    14401 tacataaatt tgtgtataat ctactatctt acaaagtaaa acaaaatctg gataaacctt
    14461 acactcttca caattatttt gtaagtacct taaatgagtt tctattttat ctacagctat
    14521 caaatccatt ttatttccta gtaaaagtac tacatcctca agagtaatcg taacaaactc
    14581 catagcatcc aaaagcaaac tcaacctttt agctccttct tctgtattta tataatcatc
    14641 atcatattca gaaaaaagcg ttacatatgt tcccttgcca ttaattgttt tagcaattcc
    14701 caattcattt agctcattat aggcacttct aactgttgga agtgatacat tatatctatt
    14761 aattatcgaa gtaattgatg gtagacaatc tcctatttta atattgcctt caaaaatttg
    14821 acaaattaca tctgaaacaa ttaagtcata taaataacat ttttccaacg ttacagaatt
    14881 aaaactactt gtatctatac tttgtggagc acgattttct accattttaa gtatggattc
    14941 tctacaagct ttatacatat atgttaatag ttttttaaat tcaattagat tcccatccaa
    15001 tccatataag taagtttttc ttacctgctc tatgtattta ttacgaaaaa agtctataca
    15061 acaattatcc aagttatcca agttatcgaa attataggcc aattgtgaaa taatgacatc
    15121 atgctgaata atctcacaaa aatggtgaat caggaaatta tttgttttac ttattagttt
    15181 atttatatat tttagccttt gttctttaaa gactaaataa ttagaactat ttttttccat
    15241 acaatcgata atctcgttta attcttcaaa gtctttagat gtaaatcttt taatattgta
    15301 catagcaata gaaggaaata ttaaactaaa aatcttatat atttctgata tttgtaaaat
    15361 cttctcatat aataagtcag attctattga ttgtttactc gtactctgga ttaaaactgg
    15421 tctagaacca gcttttgtct ctataaagcc cttttctgag agcattttta ttacagtttt
    15481 tatagtattt tttgatacca tatattcttc tgacatgctt tttatagaat caagcctatc
    15541 tccaatagta attttaccag tataaatttg ttgaattata tgctcaaaaa taattaaata
    15601 ttgtggtttt ctcttcatat ttttcctctt cgataaatta gatttattgt tttttgtaag
    15661 gtatatttac ttttatttaa tagggcaaaa tagttgctta ttttttcctc ataatctata
    15721 attaatataa accatttata tatatggtct aagttttata atactgtaat gcagtaaaat
    15781 agcatgttgt agctatacta cagcaaatta acataccatt gtagtatatt aattggaata
    15841 attttaagta cttcaaaatt ccagcaaaac atatatgtgt ttttgatatt atgtatattc
    15901 gttaatagaa tagctatact tgttttacat aataaattat atgatacttg ctatatgtaa
    15961 tcactatatt taatacacat aactattata cttgctatat gtaattacta tactttatac
    16021 acatagttat catatatgct atatgtaatt actatacttt atacacatag ttatcatata
    16081 tactatatat aattactata cttggacaca tagttatcat acatgctata tgtaattact
    16141 acacttggac acataactac tatatttact atatgtaatt atactatact tggacatata
    16201 gttatcatac atactatatg taattactat acttgaacac ataactatta tatttgctat
    16261 atgtaattat actatacttg gacatatagt tatcatacat actatatgta attactatac
    16321 ttatacacat aattatcata catgctatat gtaattacta tacttataca cataactatc
    16381 atacatgcta tatgtaatta ctatacttat acacataact attatacttt ccatacataa
    16441 ttactatatt tggacacata gttatcatac atactgtata ttaagatata atacttttta
    16501 aattttatat aacacagaca catgcaataa aaaagaaggt atccataata agctctttgc
    16561 atgtacctaa ataaaaactg taaaatagaa tatataaaat ctaatctatt ttaagtaaat
    16621 acaatttcac tttaaaagag gatgtactat gaaataccat atatttttaa tcataactac
    16681 tatattaata atcgaactat gtaacattta catatgcaaa atagcatctt ttggaaacaa
    16741 aaatataact atacataaga attacaaata tattttttta ataaaaccat taaccttctt
    16801 gataataata ttcacaaaat taccattacc actatattat atattgtttt acatagtaag
    16861 tcataaattg ctcttaagca gaaaaagttc tactaacaac cattttattc ccaatatggc
    16921 agtaataagt ttttgtattt tacaccttat tactgtaagt acaatatcac ttgttaaaaa
    16981 tacaagtcca gatataatat ttgcaaatat gacctataag ttaattagta ttttaatagc
    17041 ttctatttta gattttatct tatggtactc tatgaataaa ataaaagagc atatttatat
    17101 aataactcaa aatcaaatag agttcagtct atttttaaaa tttatctggt tcactttagg
    17161 atatatatta tttgatagta tacaaattac atttgctcca ccaagttctc taacttccta
    17221 ctttatattg ggaagtaaca tattattaat cttacaacta attatattca tcaatcatat
    17281 atattccatt gaaaaaaata tttatttaga aaaagaacat aatagactaa aaatgataaa
    17341 acaagaacaa ttactaaaag taaatttatt aaagaaaaaa gctaacattg atgaattaac
    17401 atcagcatat aatcgaaaat ttgcttttga aaaaatacag cattacattg ataacaattt
    17461 attattttca ttgatatata tcgatttgga taaattaaag acgataaatg atacatatgg
    17521 acatttagct ggggataact atattattaa cttctcaagt atagttattg aaaacttagg
    17581 aatagatgac atatttgcta gaataggagg agatgagttt atagttatta tgcctaaatc
    17641 taatgaaaac accacaaaag aatttataga gaaaataagt aaaattatgg ataatacgat
    17701 atcttttagc tatggtattg tagaagtttc tagcgaaaac aaaatgactg tagaagaaat
    17761 tattaatatt gctgatatta gaatgtacac taataaacaa aataatcata aaaggaacaa
    17821 taactaaaat gaatatttac atgagcttac ttgtagtctc actatgtttt ttatcttatt
    17881 atctctatca ttataatttt tttaaatatc tggacataat gattaataac tctaaaaata
    17941 aatacacagt atatattata tattctatat ttaattattt aatttttatt atttgtactt
    18001 atttaaagtt tcatctaatt ataaattgga caatttttat gttgatttta ttcatacaaa
    18061 ttaaagtaac ttataaaatc tccacaataa aaaacatttt ttattcacta aactgctcca
    18121 tcattggttt agcaataaat atatttttta ggtctttaat gtcaatagtg ttaaatatgc
    18181 cagcatattt atttgatagt aattctattg caaatattta cagagtattt cctatatcat
    18241 ttagctttat agttacagga ctatatttca gttataaaaa taataaaaat aataagatta
    18301 actttgaggc agttttagag gacaaaaaaa acttaatttt cttattgagc cttatgtttg
    18361 gaatgtactt gtatctatgt ttgagtttgt caatatacta tactgttgga aatgtattga
    18421 ttttaaaatt atatggaatc aagtctgcca taatagtttt ggtaggtaac tatattgcag
    18481 aaagatatag ctttagaatt actcaaataa aaggttatga tattaaaaat caaatagaat
    18541 atgtagaaag tgtaaagaca gaatatgaga ttaataaatt gaatgatatt gtatatacag
    18601 atttactgac aggtttctac aatagacctt ttgtgaatca aaaactatca gaatggacat
    18661 caaatcacta caaatttacc ttatgtttta ctgatttaaa tagcttaaaa cctgtaaatg
    18721 ataaatttgg acaccaattt ggtgataaat acatatccac tactgctcaa ataataaaaa
    18781 aacttataaa tgtagaaaat tccttactat ttagatatgg tggagatgaa tttttattcc
    18841 ttttacagaa tacaagttgt gtagaagcag aaaagataat gattaatata aattatgaat
    18901 tatcaaaatt aagtaatact gacgagtatc cttatgctct ttccataagc tatggattag
    18961 ttgagtggga tggaatatcc gatattgaaa ctttaatatc aaaggcagat tctttgatgt
    19021 atgaaaataa gcttaaaaat aaatctactt taaactagct agttaagata ttctacaaaa
    19081 tatatgttat aagagagtac taagaatatc aaaatatata gattttgcaa atattgaata
    19141 cagtttacta atgaatgcac tggatgtaag tgttagtaaa cacctattag atgaacactt
    19201 tactgttgtt tgggctaatg accgttatta tgaaatgttc agctatacct agctaaaaag
    19261 ttaaaaatga ccactgtagc cgagggaata gagaccagtt ttcaaaaaga tttcttgcaa
    19321 gaaataaact gtgatatgtt acaaggatac gtgttctcta gacctttacc aataaaagac
    19381 tttgaaaaac ttatgtttca aaactcctcc aattaacaat ataaaattgc ttattataag
    19441 tgatattatt gtatcaacaa ataaaggaat tgtctcaaaa ttgatttttt ctaatcaaat
    19501 ttttattttg agacattctc atttttttgc ataagaaaat acccaccaag ctacggtttt
    19561 tcttaccgct gttcggtggg tattagtagt attatgaaat ctgaaaactt taagtattac
    19621 tacttccgct aacgcaatca caaaggtcga aagcttcaaa gcgaaaatag gctttttact
    19681 catcaaacgt caccgtacct gccaacccgt ccaccgttat catttgtccg ctcttaatag
    19741 ttcgtgtggc aatgtgtgta gccataacgg ctgggatgcc atattcacgt gcgacaatgc
    19801 ttgaatggct aagaggacca ccaatgtccg ttacaatcgc acttgcagat gaaaataatg
    19861 gtgtccatgc tggagttgtg gtaactgcaa ccaaaacact tccaggttga aagctttcaa
    19921 aatctgctgg actattcagt acacaagctg gagccgtcac aacaccagta ctggtaccaa
    19981 tgcccgtcaa cacaattttc ccatctttct gcttctgttg tgcttgtgat atagtttttt
    20041 tgtttttctc tggcaacttg gatggtgaca tataacccat gtatttttta agttcagctt
    20101 ttcgaacagg tattatcccg ttcatatcag atagagactt gtttttatcc aactgctcta
    20161 taagtacctc caattccgat tttgtgagcc aataaatatc atctaaatgt gaaatagctc
    20221 ctcctcgtat gaatcgttct gagatttcct gaaacataca acgaatcaga ggatgtccca
    20281 ttcccatcaa ataaatagca ttttcacgca taggagaggt ttcctgtgcc caatggagca
    20341 acttaaaaaa tagtttttta cgcaatccac ctatgtgttg taaaatctct tcttctgcat
    20401 gttttctacg cttttcaaac gtggtctggc gtaaaaaagg actttcccct tttccttcca
    20461 caaatgtttt tacggattca aaagttggtg taagtgtttc ctgcggtgtg gaatgcgcaa
    20521 aatcaaactc ataagcggta cgaccaaact ctttaaagta cttattgatt cgatttttcc
    20581 attctatcca tacttcctgt gaaactccaa caggcacaac tgaagacatg aaatcttctg
    20641 ctatctttat tgcaggactg ttttgcagat aaaggttgat gctgttattt tgctttaccc
    20701 attctgaaat accccacaac tccttttccg attgcagaac aatcgtatca aacccaagca
    20761 ggaaaacgga agtatccgtt ataccagcac gtcgaactgc tccttgaaat aattttgtaa
    20821 ataatgtttc actgatagaa gcagcaggca atgtaagctg aatccttgtg aaataaatac
    20881 atgcggcata aaatattgtc tgaataccct ccataatttg atgggcgttc atagtatgca
    20941 ggggcttttc ttcccattgt ttaatagcat cctcatatcc ttttcgtgcc gtttcccagc
    21001 gtgcaacgct gttcataagt gtcctacgta aagagcgtgg ggaaagggat ttgatagcaa
    21061 tcaaaagtgg ctttgattta gcagaaagat aaacatatcc attaataacc gtatatgccc
    21121 cgttttttgg taataatttt ttcgcacttt taccaaacat atctatccat aaaagctctg
    21181 atgcacggtt tacgatttca agaccaagtg tggcgaacaa aggtgtaaca ggatttggca
    21241 aatgttctgc caaacttccc tttgtgtata caacatcctg ttctggcaat acccattctg
    21301 gtggcagaac agtaattgga cgagcctgaa caatgtacaa tttatctttt tccagtgccc
    21361 actctacatc cataggcatt tgataatact tctcaatttt ctttccaagc tgtatcagtt
    21421 gcattacctg attacgagta agagcatatt tttttctcaa cctttcagga accatggttt
    21481 cttctgttcc ttctgaagta cgaaccgtca taatctcttt atttgccact tcatacgata
    21541 taattcgttc ggagtcctta tccactacga ttgtatcggg tgtaactaag ctagaaacca
    21601 ccgcttcacc aagcccccat gcagcgttaa taatcatttc gctacgtcta ccattgattg
    21661 ggtttaacgt aaacataata ccagatgaat cggaaaatgc aagcttttgt actacaacag
    21721 caagtgcgac aatctcgtgc tttatattat tcttcacacg ataagcaata gcacgagctg
    21781 tccataagga agcccagcat ctctttacag agtcaataac tttatcttca ccttgtatgt
    21841 taagataagt ttcctgctga cctgcaaaag aagcgtcagg taaatcttca gcagtcgcag
    21901 acgaacggac agccaccgat atgttcccta atccagcata agccatttta attgcgtccg
    21961 acacttcctg tggcatttct ccattatgga aaagctcccc tattttctta gatacatctt
    22021 caagttggct ggtattattg gaatcaatac catctagcag tttattaatt cgagactgaa
    22081 tatggttcgt ctcaacaaaa atctggtatg aagcagtcgt aacatgaaaa ccgtctggca
    22141 cagggatgcc tgctgtcaat agttttgata aagacatccc ttttccacca acaatttcaa
    22201 tggttgcttg tttatgttcc agtggcaaga cataataaat cataatcatt cacctccatc
    22261 attcataatt ccgttcagaa atatattcat tgtttcatcc aacatttttt ccgaactatc
    22321 caatggtcgt gacgtgtagc tgattgataa catggagtta attaacaaac gtgtcacaag
    22381 agcggtatcc gttttgcgaa agatgcctgt ggtcacacca ttatcaatta ccgattttat
    22441 aatatcttga tatgcataac gagcatcttt aagtctcttt cgatactctt cttctaaata
    22501 atcagattct gtatttaacc acatcagtac agttctatac tgctctggaa atctaagatg
    22561 attaagcatg atttttctaa ggcactgttg tggtgaaggt tcaccatcaa taatcctatg
    22621 aacttgttgg atagtttttt ctatttcttt ctcaactgca tatacaacaa tctcgtcttt
    22681 cgttttaaaa aaatcgtata gacttgactt tcccatgttg gctaaaactg caatttctct
    22741 cattgatgtt ttttggaatc cattttcagc catgagactc aaagcaacag caactatttt
    22801 ttctcggcgt tctgcttgtt gttcgggtgt aagcacaatt ccttttggca tagtgcgata
    22861 cctcctttta gcgaccactg gtcgtttctt ttttatttat catacagcaa ccaacggtca
    22921 cttgtcaagg ttaatttttt tcattgtata tggtactcga gataaaaatg ttcattttag
    22981 aaattactct atttaagtag aaataggctt catcattaga aagcaagact ataaatttga
    23041 gatatttatc atgttcaaat ttcatcaata tcaaagttta aaatattaat aaaaaaagaa
    23101 ttactaagaa ataacatata aagattcaaa aaaggagttg aacttaaaat tcaattacaa
    23161 aaaatcaaat ttaagacaac tcctttattc tttatctcta ctattcttta tctctattta
    23221 tttattacaa ctccctaaca tttaccacta ctgccttagg tctagtcata gcctcaattg
    23281 aatacttaac accttgagtt cccataccag atgcctttac acctaaaaat gggaagtggt
    23341 ctggacctct ttcagtctta ttatttattt gaacagtacc aacttcaagt ttatcagcta
    23401 tataaaatgc cttatcaata tcacttgtaa atactgatga ttgaagccca tactctgatt
    23461 gattagcaat ttcaattgct tgatttatat ctttaactct tataattgga agtactggac
    23521 caaatggttc ttcccaagca attttcatat caacatttac attatctaac aatgttggat
    23581 agaataaatt attttttcta acattgcctg ttattaactt tgctccttta tgtaatgcat
    23641 catcaaccaa cccttgaaca aaatcagttg ctttattatc tataagagga gtgataacta
    23701 cttcatccat tggatttcca attgtcaact tctctaccaa tggctttatt tttccgacta
    23761 atttatccgc cacactctct tgtactaaaa ttctctttac tgccgtacat ctttgtcctg
    23821 aatatgaata cgctccagat actatattct tagctgcaaa atctaagtct gcatcttcta
    23881 atactatagc cgcatctttt ccaccaagct ccaataataa tggtaccatt ccagatattt
    23941 tagatatatg ctgaccaact tcagtactac cagtaaagtt aataaaatta attcctttat
    24001 gcgttacaac ataatcccct atctcactac ccctacctgt aatagtatta agtactccat
    24061 ctggtatccc agcttttctc ataatttcta ccacatgaag tgcgctaata gccccttgtg
    24121 tagcaggttt taatacgact gcatttccac caataagtgc tggagcaatc tttgacattg
    24181 ataaatttac aggataatta aaaggagata tagctaaaac agtacctaat ggaacccttg
    24241 aaacataaga catcttattt cttgttccac cagggaaatt atctccacta atggcttctc
    24301 cctccatatt ttttcccaca tcagctgtgt atcttaaaaa atctgctgtt ctctcaactt
    24361 ctgcccttgc agattttatg tctttggcta tttcaagtac cagtatatta gctatctcat
    24421 ctatattttc taacaataaa tcagcagcct tatgaaatat attgcctctc ttgtatacag
    24481 gcatttcagc ccatactttt atactctctt ttgtgttttt tataacctca tctacctcat
    24541 gttttgtcat agattgtact ttcccaacaa gagaattatc tattggtgag taaatttcta
    24601 ttggctttct cgaattagat tctacccatt ttccatttat taaattttta tatacatttt
    24661 cttttgtttt aagttcatta aacatatttt tattcctctc tctgatataa ctttgtgtat
    24721 ttaaattata gccatttatt cgttatgtaa acatttcttt ttttcataaa cgtttaccta
    24781 taaaaaaatt ttaagttaac gtttttctac attcttctat tttaataaca tactcgctcc
    24841 aaatctatat aggtcacact ttaatactaa tatatcaata taccaatata ccaatatacc
    24901 aatataccaa tataaatatt ataaacataa atcacttgac aaatttataa gtagacaata
    24961 ttatatatct atgactcaaa atatttttaa agtcatatat atcatatata gacagaaagg
    25021 attgataaaa atggctaaaa gttttacaga aatagaaaaa gacaatataa gaaaaagact
    25081 aatttcagaa tgtgaaatca actggtctaa atatggatac aaaaaaacta atattgatga
    25141 actttgttca aaatcaggaa ttgcaaaggg ttctttctat acattttttg attcaaaaga
    25201 aaagttattt tttgaagtat taaatggaat ccaaaacaga cttatatcat cagtagatga
    25261 aattttaagt aacatgcctc caaaagaagg ctttgcaaaa tccttaaaaa tgttatatcg
    25321 attatatgat aaaaatccat ttttatattc accaaataat cctgatttca tcagcctaat
    25381 aaataaacta cctcaagata tgtttttaca acttgagtcc aataacatac aaagttttta
    25441 cgacttaatt gagagacaca atctcaagtt gaagatagaa aaagaaaaag tttttggtgt
    25501 ttgtaatgct ctactatcaa gtctgttggt aaaagaaaat attgaatata actatattga
    25561 agtgtttgac tttatggtgg ataatcttgt agaacacata cttgaataac aatttaaaat
    25621 aatttttcta agtatttttt tatactaaat aggattaata tgattgaatt aatgcaaaaa
    25681 ataaacaata taatttataa aataaatgat aaactaaata tcacacaaaa cttatagacc
    25741 ctagagcaaa cccattccac tctactctaa gccatctata atagtttgta tatttctaat
    25801 aatattagat attaaagtaa gaaaaaaatt ccttataata attgctatta tatcaattat
    25861 tgtaaatgtt ttaataatct tagataggta ggtacatata tggtaaaatc ctcgaacact
    25921 tttaataaaa acttcaactt gatgatatta ggtcaaataa tctcattgtt tggtgcatca
    25981 atacttaaat ttgctttatc tttatacata cttgatataa ctggtaaagc agaaatattt
    26041 gctactatac tagcagtatc ttcaatccca attatagtat tctcccctat tggtggagct
    26101 attgcagata tattcaacag aagaaactta atggttattt ttgatttttc aagtagtctt
    26161 acagtactaa tcttagcagt attcttattc aacaataatg gttcaatatt acttgtagga
    26221 attattatga ccatattatc agtcataagc acaatgtatc aaccaactgt tcagtcaagt
    26281 acaccccttt tagttgataa tgagcattta atgaatgcaa atggtattgt agctggagtt
    26341 gcatctctta caaatattgc aggacctgta ttaggaggtg tcttgtatgg tgtgattgga
    26401 attaatgcaa ttatagttat aagttgtgta agtttctttc tatctgcaat tatggagata
    26461 tttatacaga ttccttttat caaacaagag caaaacagca acattataaa aactatattt
    26521 actgatataa aggatggtat tgttcatata tgtaaagaaa atagatttat actaaaaata
    26581 ctttttttag ctatagcaat aaacttattt ttaagctcta tgttaatagt tggtatcccc
    26641 tatatagtaa aaatcactat gggagctagt aacttcttat atggtctagc agaaggagca
    26701 atttctttta gttcactggt agctgctgtt agtattggta ttttctcaaa acatctaaaa
    26761 atcactaatt tgcatatttc ctttattata tgtgcagtta tgttcatacc aatggcggtt
    26821 tcagtatatc catttatatt aaacacagga tttccagttc catttttaat ctttatagta
    26881 tcatctatgc taatattttt tacaacgact cttatctcaa tatatgttat aacagtcaca
    26941 caattaaaaa ctccaaatga actcttagga aaagtaatgt caattctttt tgcaggaaca
    27001 gcaattgcaa tgccccttgg tcaggttatg tatggtaaac tttttgattt attatcagat
    27061 agagtgtaca tcattatact aggagttgga tttattacat tacttatagc atttgtatca
    27121 aagataacat tgaggaacga aaatctttag atacaaagaa tttagatata aagatttaat
    27181 tttcactaat ttctgaattt aaatgcaaaa ttgtgtatca gtatgttgac gttttagaaa
    27241 ttgattgata taatcaatgg aaaataaact ttataaaaat tcaaaatatt cagctagagg
    27301 agtgataaaa atgagttaca tgccaacttt agaacaagca tgggaaatat taagaaaata
    27361 caataaggaa gaatttcata taagacatgc tcaaattgta tctggagtta tgagatacta
    27421 tgcaaaggag tacgaccctg aaagagtaga attttgggaa atagttggac ttttacatga
    27481 tttagatttt gaaatgtatc ctgatgaaca ttgtgttaaa caaagagaat taatgacaga
    27541 gcttgattta gataaatcaa taatcgattc tacaataagt catggatatg gattaactgg
    27601 ttcagatgtt aaaccagagc aatttatgga gaaagtatta tttgctgtgg atgaacttac
    27661 tggtttaatt ggtgctgctg caataatgcg tccttcaaag agcgtttctg atttagaatt
    27721 aaaatctgtt aagaagaaat ttaaagataa atcatttgct gctggctgtt ctcgagacgt
    27781 tataagaagt ggtgcagaaa tgcttggatg ggaacttgat aagcttatcc aaacaactat
    27841 agaagctatg agaagcttga ttccagagat ggcaatatag ctcctccata catataccat
    27901 agaagttaca tctttaaaga gcatttctct ttttgatgca acttcttttt atattttgta
    27961 gaataaaaag tttaatattt taaactcact atataataaa taatataatg tataatagtc
    28021 ataaactttc taaccttaaa agtttctaat aatcaataaa caattctaat atacaaatgt
    28081 cattatatga tttaattata tatcttcaat ttatatatt
//
LOCUS       DAMJYR010000039        27681 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.039, whole genome shotgun sequence.
ACCESSION   DAMJYR010000039 DAMJYR010000000
VERSION     DAMJYR010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 27681)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27681)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27681
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.039"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            279..623
                     /locus_tag="PC471_001722"
     CDS             279..623
                     /locus_tag="PC471_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HDF2788853.1"
                     /translation="MNNKEKGDFGEKVAVNYLLSKGAKILEKNYRLKIGEIDIIAKME
                     DEIIFVEVKSRSNIKFGYPCESVSFKKRKKIIGVANYYIIKNNLNNTPIRFDVIEVYL
                     LEKRINHIMNAF"
     gene            654..2255
                     /locus_tag="PC471_001723"
     CDS             654..2255
                     /locus_tag="PC471_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HDF2788854.1"
                     /translation="MLSIINSSNLVGIDSFLVKVEVDVSNGIPSFNIVGLPGKEIKEA
                     RERVKSAILNSGYKFPSTRIVVNLSPADIKKEGAFLDLSISIGLLRELIKKDENYIRE
                     SMFIGELSLDGKIRKVRGILPIIMGAKTQNIKRIFIPIENIKESLFVDEIDIIPIKSL
                     KECVDFLNEEIKVDKVRIMSFLDDKSRKENEELEKDNSYIDCKYTKINNEESKYDEDF
                     KDVKGNYFVKRSAEIAAAGNHNMFMIGPPGSGKTMIAKRVRTILPDISVEEMIEVSKV
                     YSILGMINETKGIIDKRPFRAPHHTTTKQSLIGGGMDARPGEIALAHRGILFLDEIAE
                     FDRKILETLRQPIEDGYVNISRVKYSAKYPCRVLLVAAMNPCPCGYYMSETECRCRSN
                     EIDRYINKISGPLLDRFDIFVEVNSIKYSDFNSLKQEESSQKIKRRVENARKIQINRF
                     KKDNIKNNSEIKAYNLFKYCKLEKEASKTAEMIFSKYNLSSRSYTKLLKMARTIADLE
                     ERDLINSQCIIEAFSFRKAYYSYFK"
     gene            2274..3374
                     /gene="dprA"
                     /locus_tag="PC471_001724"
     CDS             2274..3374
                     /gene="dprA"
                     /locus_tag="PC471_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HDF2788855.1"
                     /translation="MEKRDTYLWLKSIGGITTKTIEIIENEIVNIEDIFDFSEKEIYN
                     LKNISLNIRKNIVKYRGHAYLENIKELLYKKAIKYICKYDKEYPENLKNIYNAPKLLF
                     YKGDIGLVNNNFNIAIVGSRKPTAYGINCAKTISCQLSQYGVNIVSGLAIGIDAYSHI
                     GCMSGKSKTIAVLGSGVDNPLPKQNLHLSNKILENGGLLLSEYNINSTVAPYHFSNRN
                     RIISGLSDGVVVVEAAIKSGALITVDFALEHGKNVFAIPGNINSQMSRGCHKIIKEGA
                     KLIENIDDILNEYNIFNIIDKKINQKYDNISLNAKSKQIIEAIKREGNLHIDSICDYT
                     GIEIKYVNSIINELVLNELVVEMNNKTYSLNV"
     gene            3407..5494
                     /gene="topA"
                     /locus_tag="PC471_001725"
     CDS             3407..5494
                     /gene="topA"
                     /locus_tag="PC471_001725"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HDF2788856.1"
                     /translation="MAKTLVIVESPAKAKTIEKFLGKSHYTVKASVGHVRDLPKSKLG
                     VDIENNFEPQYINIRGKGDVIKELKKEAKKSKQVYLATDPDREGEAISWHLAHILNLD
                     ESEDCRIEFNEITKDAIKKAIKHPRNIDINLVDAQQARRVLDRLLGYQISPILWQKVR
                     KGLSAGRVQSVTTKLICDREKEIKAFIPKEYWTIDVEAKTVKDKDITLKFYGKNDEKI
                     ELENEEAVNEILKEMEGKDLKVEKIESKSRRKSAPKPFTTSMLQQEGANKLFFTTKKT
                     MMIAQELYEGIDIEGEGTVGLISYIRTDSKRISDEAKEKAKGYIVNNLGENYYKNIGD
                     KKEKKDTKKVQDAHEAIRPTSVDRTPESIKASLSKDQYKLYNLIWRRFVASQMEDSVF
                     DILNVECKIGDIVFKATGSKMKFDGYTKVYNFSEREDKILPSIEKGDILNIENILPDQ
                     HFTQPPARYTEASLVKTLEELGIGRPSTYAPTIATILNREYVEKQGTSLCPTELGIIV
                     TDILENNFHKFIDVDFTADMESKLDVIEEGNTDWKKIVSESYAPLKEAIEEALENIEK
                     VNMDEETDEICENCGSNMVIKYGRFGKFMACKNYPDCKTTRPIMNKIGVKCPKCEEGD
                     IILRKSKRGKAFYGCSNYPNCDFVAWNKPTGEFCEKCGSYMVEKVTKSETKTVCSNKE
                     CNKETKEHNDGKE"
     gene            5659..6444
                     /gene="codY"
                     /locus_tag="PC471_001726"
     CDS             5659..6444
                     /gene="codY"
                     /locus_tag="PC471_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HDF2788857.1"
                     /translation="MASEVLQKTRKINKTLQTSGGSSVSFDLLAGALGDVLSSNVYVV
                     SAKGKVLGLHLNDVQDSSVIEDEYTKQKKFSDEYTQNVLKIDETLENLNGEKILEIFP
                     EEHGRLQKYTTVVPILGSGQRLGTLVLSRYSNSFNDDDLVIAEYSATVVGLEILRAIG
                     EELEEEMRKKAVVQMAIGTLSYSELEAVEHIFAELDGKEGLLVASKIADRVGITRSVI
                     VNALRKFESAGVIESRSLGMKGTHIRILNDKLTDELKKLKNNQ"
     gene            6551..7816
                     /locus_tag="PC471_001727"
     CDS             6551..7816
                     /locus_tag="PC471_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HDF2788858.1"
                     /translation="MPLADKIRPKTMNDVIGQSHIIGNGKILSKILQSNFLPNMIFFG
                     PPGVGKTTVAEIIAERSNKSFYKINATNSSLEDIKKVISELGSINNVNGVLLYIDEIQ
                     SFNKKQQQSILEFMENGSITLIASTTENPYHYVYKALLSRSTVFEFKPLEKLDIEKGL
                     KRAVEVLNEDSYMDIECNNDAIEDIAILSDGDMRRALNILEVVVYSTKPNKDNITYID
                     SDVIRASTFNKIINYDKNGDSHYDILSAFQKSIRGSDPQASIHYLARLIKGGDLISIC
                     RRLLVIASEDIGLAYPNAIVIVKACVDSAMQLGFPEAKIPLAEATILLATSPKSNSAC
                     IAIMKAMDELDKEFVKDIPNSIKDAHYSGSKEIGRGCGYKYPHNFKNHYVKQQYLPDS
                     IKDSIYYIPQENKTEKNIKKYLEYLNSDF"
     gene            7847..8617
                     /locus_tag="PC471_001728"
     CDS             7847..8617
                     /locus_tag="PC471_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDF2788859.1"
                     /translation="MIIKLNSTYHSRVMKYLKKEPEYNLFIIGDIERYGYGNNFLNIW
                     ADVGEHGEIKAILLKYFEFMMFYSDGEYDVEGFYNLLRNTNYEEISGKICAVDALAKR
                     LGLNNLKVVDFCKLQTKKFLIDNNCNAKVKRIRLGNLKKTVKLYDLIDEFHSTTLENL
                     KNGLRTGRGYCIEINKQVVSMAKSTSENRTHAMIIGVGTHPKYRAKGLATKCLIKLCS
                     ELLRENKIPCLFYDNEEAGKIYKKLGFENIGKWGIYSK"
     gene            8726..9172
                     /locus_tag="PC471_001729"
     CDS             8726..9172
                     /locus_tag="PC471_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HDF2788860.1"
                     /translation="MKLSTKGRYGLKAMFELALNQDNGPVSLKFIAKKQKISDQYLEQ
                     IFSSLKKSGLVKSVRGAQGGYLLSKNAEDITVGDILVVLEGPVALSDCVLDEDVCENS
                     NMCVTKIVWEKMKKGIEDVIDSITLKDMINDYNKNKLENDITNIKK"
     gene            9190..10383
                     /gene="nifS"
                     /locus_tag="PC471_001730"
     CDS             9190..10383
                     /gene="nifS"
                     /locus_tag="PC471_001730"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase NifS"
                     /protein_id="HDF2788861.1"
                     /translation="MEKRRLYMDYSATTPIKKEVLDAMMPYLTDYFGNASSFHTFGRE
                     AKDALDKAREQVAALINAEPSEIYFTAGGSESDNWTLEGVAYANKNKGNHIITSKIEH
                     HAILHTCEYLAKHHGFEITYLDVDSEGKVDLKQLEDSIKDTTILISIMFANNEIGTIQ
                     PIKEISEIAKKHKILFHTDAVQATGNIPVDVKELGIDLMSMSSHKIYGPKGVGALYIR
                     KGVRLHNFVHGGAQEKSKRAGTENIPAIVGYGKAAELAKENMQNHVETLTRLRNKLID
                     GVLERIPYTRVNGSLENRLPGNANFAFQFIEGEGILLLLDMLGIAGSSGSACTSGSLD
                     PSHVLLAIGLPHEIAHGSLRLTVGDFTTDDDIDYILENLPKVIERLRSMSPLYDDAKK
                     QGLVK"
     gene            10437..10877
                     /gene="nifU"
                     /locus_tag="PC471_001731"
     CDS             10437..10877
                     /gene="nifU"
                     /locus_tag="PC471_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold protein NifU"
                     /protein_id="HDF2788862.1"
                     /translation="MQYSDKVMEHFMNPRNMGEIDNASGVGEVGNPTCGDIMKIFLDI
                     DGDVIKDVKFKTFGCGSAIASSSMATEMIKGKTIKDALELTNKAVAEALDGLPPVKMH
                     CSVLAEQAVKAALIDYAQKNNIHIPELDGYVIDDAHDHDVEEEE"
     gene            11166..12245
                     /gene="mnmA"
                     /locus_tag="PC471_001732"
     CDS             11166..12245
                     /gene="mnmA"
                     /locus_tag="PC471_001732"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HDF2788863.1"
                     /translation="MNKKVMIGMSGGVDSSVAAYLLKQQGYDVIGVTMKLWQDDDVVE
                     IEGGCCSLSAVEDARRVANKIGIPFYVLNFREVFKEKVIDYFIDEYLEGKTPNPCIAC
                     NKHIKFDDFYKKARQIGCDYVATGHYAKIEKDESTGRYLLKKSVTDKKDQTYALYNLT
                     QEQLEHTLLPIGDYEKDRVREIAKEMGMAVHNKPDSQEICFVKDNDYANYVKKHSKKR
                     IEEGFFVDTKGNILGKHKGILYYTIGQRKGLGITFGKPMFVIDINPINNTIVLGDNED
                     LFKKELIAKDVNFISIDTLEEPLRVQAKIRYSAKTSPATIHRVGEDTIKIVFDEAQRA
                     ITKGQSVVMYDGDIVVGGGIIEKSL"
     misc_binding    12388..12626
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            12694..15333
                     /gene="alaS"
                     /locus_tag="PC471_001733"
     CDS             12694..15333
                     /gene="alaS"
                     /locus_tag="PC471_001733"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HDF2788864.1"
                     /translation="MEKMGLNEIRSKFLEFFESKGHYVANSYSLVPNNDKSLLLINSG
                     MAPLKNYFSGVEVPPSVRMCTSQKCIRTGDIENVGITARHATFFEMMGNFSFGDYFKR
                     ESIKWGWEFVTEWLNIPEDKIWVTVYEEDDDSYDIWAKEMNFPEERMVRLGKDDNFWE
                     IGTGPCGPCSEIYFDRGEEYGCDNPDCKPGCDCDRYLEFWNHVFTQFDRDEEGNYSLL
                     ENKNIDTGMGLERMGCIMQGVDTIFEVDTIKSILEAVEKLTGVKYGENPKNDISIRII
                     TDHIRAVTFLVSDGVLPSNEGRGYVLRRLLRRAARHGKLLGVKELFLQKLIDEVIKVN
                     DKAYPVLVEKESYIKKVVGIEEEKFNETIDQGTEILNSYIEVLKNEGKTVLSGQEAFK
                     LYDTYGFPIDLTKEILEEEHLSVDEEAFNEEMEKQKERARNARGNMDGESWKEDPLSK
                     LESTVDSTFNGYSEIYGEGTIEAIVKDDELVQSAEEGDKVSIVLDNTTFYPEGGGQVG
                     DCGLITNENLVLEVLNTKKGANNSIKHIGIIKSGRISNGDKVKTLVDRETRMSAARNH
                     SATHLLHKALREVLGEHVNQAGSLVTPERLRFDITHFEAISNEELKVIEEKVNNVILS
                     SLDIKCDIMNIKEAKEKGATALFGEKYGDEVRVVSMGDYSTELCGGTHLTNTSQVGMF
                     KILSEGGVAAGVRRIEAITGKAVYEYLKERDGIISEVCVNLKSKEDNLIQRISSLLEE
                     NKNLSKELHDMKAKMSLQSVDSIFDSKVEVNGVNLITNKFEGMDMDTLRETADNLRDK
                     LGSGVVVLANVVDDKVNFVVTATKDVLDKGIHSGNIVREVAKIAGGKGGGRPNMAQAG
                     ASDVSKVDQALSYASEVIKTQVK"
     gene            15358..15615
                     /locus_tag="PC471_001734"
     CDS             15358..15615
                     /locus_tag="PC471_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HDF2788865.1"
                     /translation="MEKDLDYTMKFEGIPEDRMSVGDTIDFVYKALVEKGYNPINQII
                     GYLLSGDSSYITSHKNARAIIKKFERDEILEEVITHYLNRK"
     gene            15625..17796
                     /locus_tag="PC471_001735"
     CDS             15625..17796
                     /locus_tag="PC471_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2788866.1"
                     /translation="MRKKIMSLFAIVIMIFVSTASYGFAEEKGKVIFIDMNRTNLGSM
                     MNIPILKNEIEKRGYVALMNIRGDQGTDDRRSYASMGAGTRASVTTEDYINFQKVDQD
                     TAKAFEVATGQKAKEINDLGINRSINENLEYGEYGANLGSLGKTLSENGLKTSVIGNA
                     DIIENNQLVKNRNLCLVAMDQYGRIDSGNIDNINKKDSKMPFGISTDYDKLLDETKKS
                     YKNSDAIFVELGDTYRLDMYRLNLNDNTYDIMKKNIVKNINKYLEQVFSMVGENDTVY
                     IASAFPSDLDYKNKRRLSPIIKFSGEGKGLLESATTRKAGIVANLDVGVDILGTFGLE
                     NKEMVGRSYTLVNKEDNISYLKSLYERIVSISSIRSTIINGFVGVISASWVIAMFAIF
                     FRNYMPHKERVFKVLKEFIKLGIIMPLSFMLAPLMNFKTPISISLGIFATTLILYLLG
                     RKLFKDNDIKHMGFFATLTILVIVIDSVFGTYLMQNNIMSYDAVIGARYYGVGNEYEG
                     VTIASAVFGLAVLLHYKKVSKLLVVIFSLIILITSAYPSMGANVGGAISECVAYLLFI
                     MLIYDIKIDFKKAVLLAVSAVVVVFAFAALDILSGSESHLGMFVQRIFLNGPGEILQT
                     FGRKIQMNMQLAQTSVWVNILLVGIGVIAVLIFKPSRHFRKIMNNFPILFKGFIASMV
                     GCIITLLVNDSGIVAASTASIYILIPLIIISINMIIFEKNN"
     gene            18002..18427
                     /gene="ruvX"
                     /locus_tag="PC471_001736"
     CDS             18002..18427
                     /gene="ruvX"
                     /locus_tag="PC471_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HDF2788867.1"
                     /translation="MLDGRIMGLDVGDKTIGVAVSDLMGLTAQGVKTIKRVGKKKDIE
                     ELKAIIKEKQVNKIVSGLPKNMNGTLGPQGEKVIKFCELVKAETGIDVEFWDERLSTV
                     AAERSLLEADVSRQKRKKVIDMLAAVIILQGYLDFKINS"
     gene            18505..18792
                     /locus_tag="PC471_001737"
     CDS             18505..18792
                     /locus_tag="PC471_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HDF2788868.1"
                     /translation="MEENIINLIDENGVESQFEIILTLEAEGKEYAILMPLDDEEAEE
                     ALIFRIDEDEEGEILIPLESDEEYETVVAVYTAIMEEEGLNYDEDESNGLN"
     gene            19074..19532
                     /locus_tag="PC471_001738"
     CDS             19074..19532
                     /locus_tag="PC471_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HDF2788869.1"
                     /translation="MANTMDLLKDKLKETGFKITPQRRAIVEILLKHDHSHLSSEEIY
                     DLVRVDCPEIGLATVYRTMQLLDEIGLISKLNLDDGCIRYEISLHKEDCHNHHHLICK
                     NCGKIMEAKEDLLDNIEKEIQSLYKFKILDHDVKFYGLCDECNGVSDSEE"
     gene            complement(19649..20386)
                     /locus_tag="PC471_001739"
     CDS             complement(19649..20386)
                     /locus_tag="PC471_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HDF2788870.1"
                     /translation="MRGKTHCAIGILTAIQTSLIFKIPISLVDILVSATFAVLPDLDK
                     SNSMVSNFILKNNVSKYIYRIFIYAVNIIIFFISININDNFYLSAIITFVAIIIIEAK
                     LTHTFLRKVFLSLIFILLAICLYIIEVEIYFTIFCLMLSIFPWLKHRSFSHSIFATII
                     VYFLLKQIELITNINNLSFYGTIGYASHMFLGDLFTKQGIPIFYPLSEKKISLGFLTV
                     GGPFSNFIEKSFIFVLIGLIIFSILKL"
     gene            20603..22279
                     /locus_tag="PC471_001740"
     CDS             20603..22279
                     /locus_tag="PC471_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HDF2788871.1"
                     /translation="MQLFKKNTNKIKVMALGGLNEVGKNMTVVEYKDEIIVIDAGLSF
                     PEDEMLGVDIVIPDITYLVKNRDKIKGIFITHGHEDHIGALPYILKKINVPVYGARLS
                     IGLIQVKLKEHKMNNVKLNVIGPRQVIKLDNMEVEFLKNNHSIPDAYSIAIHTDQGII
                     YHTGDFKIDLTPIDGDVMDMHRICELSKKGVLLMLADSTNAEKPGFTMSEKTVGVGLD
                     ELFAKGNGRRIIVATFASNIHRLQQIINTAEKFNRKVAISGRSMVNVVGVAKELGYLD
                     ISDDMLIDLNDICKYEDSELVIITTGSQGEPMSALARMAFSEHKKVEIKSGDLVIISA
                     HPIPGNEKLISRVINFLFEKGAEVVYSDIADIHVSGHACQEELKLIHALVRPKFFMPA
                     HGEYRMLKRHAEIAEQLGMDKENIFVMQTGDVLELDKNSAKVANRIQTGNILVDGLGV
                     GDVGNIVLRDRKHLSEDGLMIVVVTISKDEGKVLAGPDIISRGFVYVRESEDLMDGAK
                     DIIKNVLNECEEKNIKEWAYLKNNIKENLKEYLYQKTKRNPMILPIIMEV"
     gene            22485..22685
                     /locus_tag="PC471_001741"
     CDS             22485..22685
                     /locus_tag="PC471_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore protein"
                     /protein_id="HDF2788872.1"
                     /translation="MDDVSRQNAIKALKQTKMEIAGEYGMNYEDAFEIIENASNKGVL
                     EGYFKKLEKKKNLGQGISRHLE"
     gene            22739..23902
                     /locus_tag="PC471_001742"
     CDS             22739..23902
                     /locus_tag="PC471_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HDF2788873.1"
                     /translation="MKKLASTALAILIALTPLSFSFANNKENADANQLNISSKSAILM
                     DVGSGQILYEKDAHKKLPPASVTKVMTMLLIVEALDSGKIKLDDEVQVSETASSMGGS
                     QIFLEPGETQKVDTLLKGIAVASANDACVAMAEHLAGSVEGFVDRMNAKAKELNMNDT
                     HFANTNGLPVANHYTSAHDIALMSRELLKHEMISKYLTTWMDKVVVGKKQVTVGLANT
                     NKLIKHYQGATGVKTGFTQEAKYCLSASAKRGNTHLVAATLGAETSPERFNDASSLLN
                     YGFANYESVKLCSKGDNIATLTLDKADENKVKLVAKEDLNALIKKGSSKEFEKKIEIV
                     KNPKMPIKKGTVLGKIKICKDKKVIGEVELINTKDINKASYLQMLQRIIDNMI"
     gene            complement(24113..24535)
                     /locus_tag="PC471_001743"
     CDS             complement(24113..24535)
                     /locus_tag="PC471_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2000 domain-containing protein"
                     /protein_id="HDF2788874.1"
                     /translation="METKNSKCVMVIDENLPMGIISNTAAIMGITLGKHAPETVGPDV
                     IDKTGNSHLGIIDIPVPILKGNKEIIKDLRKKLYTLEFNDLTVVDFSDVAQSCNLYEE
                     FTQKIASVPEDELQYFGIAIYGNKKKVNKLTGSMPLLR"
     gene            complement(24556..25392)
                     /locus_tag="PC471_001744"
     CDS             complement(24556..25392)
                     /locus_tag="PC471_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HDF2788875.1"
                     /translation="MRQEERTVCFDRELKIEAYRFKGIMQKFPNHFHEHYVIGFIEKG
                     QRYLSCKNKEYTTSTGDLLLFNPFDSHTCEQIDDKVLDYRCINIKPEIMKKTVFEITG
                     KNYLPKFNQPVIFRSELVPLLQELHYIIMEEELDFKKEELFFFLIEQLIEEHTEPNLQ
                     SNLENTNIEIQAVCDYLENNYAEHIVLDELSTIAGMNKYSLLRNFTKLKGITPYRYLE
                     NIRVNKAKKLLEKGVEPIDAAIQTGFVDQSHFTNFFKNFIGLTPKQYQNIFINDDSNN
                     FK"
     gene            25690..26286
                     /locus_tag="PC471_001745"
     CDS             25690..26286
                     /locus_tag="PC471_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HDF2788876.1"
                     /translation="MLSMNIGEIVASLVGIAMAISIHEFGHAYSAHLLGDDTAKAYGR
                     MTLNPVRHVDPIGLLAMFILKIGWAKPVPVNPNNFKNYKIGNLIVSLAGVFCNVLTAI
                     LCVIINKYVHMYAINTIAEYVFLYSIGFAAFNLLPIPPLDGWGVISTFVPYKWNEYLY
                     KFESMSYIILLIALFTGMYRIILTPIYAVLLKIVYLFV"
     gene            26316..27035
                     /locus_tag="PC471_001746"
     CDS             26316..27035
                     /locus_tag="PC471_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HDF2788877.1"
                     /translation="MKYNIQLQVYEGPLDLLYDLITKHKIDIKDISIIDITKQYLNYL
                     KMLDKMDLEITSEFITMASKLLEIKSKYLLYKQKDEEEDPRIELMEKLEEYRKFKVAS
                     QDIKENITYVNERFYRNKEEIIIDDNVDLEDISIEAIKNILPYIFKVKTSQIENANDE
                     KLDKIVRKKIISVEEKILYIRDIIKEKIEVTFTNIIKSYENDEIIATFLSILELIKEK
                     EIVVVQDIFFDDILIRKSSEC"
     gene            27037..27576
                     /gene="scpB"
                     /locus_tag="PC471_001747"
     CDS             27037..27576
                     /gene="scpB"
                     /locus_tag="PC471_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HDF2788878.1"
                     /translation="MKREDIKYIIESVMFAYGEPISIKELNYIINKELSSKEIEIMLN
                     LLIEEYREQNRGIQIIKLENKYQMCTNKDYAEYIKKIIEPKKKKSLSQATLETLTIIA
                     YKQPITKVEIEDIRGVKCDKVLQTLFENELIREAGRLNKIGKPIIYKTTDEFLKLLNI
                     ESLEELPPIENYQEVATNE"
BASE COUNT        11216 a         2891 c         4993 g         8581 t
ORIGIN      
        1 ggatacaatg tattttcaac atttttcaaa tagcttacaa ctaatataaa taaagataaa
       61 tatcgtataa aagcaactaa tttttctctg ttcattaaag gcactcctcc aaaaaaataa
      121 acttttccta tgaaaactag aataatatca taaaagaata aagtcaatag aagtatttat
      181 taatattgat ggaaataaaa tcaatccaaa aacttgaata gtaaaattgt atactgacca
      241 taatttaaaa gtgaaaattt aatgtattgg agtgaataat gaataataaa gaaaaaggtg
      301 attttggaga aaaagtagca gttaattatt tgctatcaaa aggagcaaag atactagaaa
      361 aaaattacag attaaagata ggggaaatag atataatagc aaaaatggaa gatgaaatta
      421 tatttgtgga ggtgaaatca aggtctaaca taaagtttgg ctacccttgt gaatctgtaa
      481 gcttcaaaaa aagaaaaaag ataattggag ttgcaaatta ttatattata aaaaataact
      541 taaataacac tcccataaga tttgatgtta tagaggtata tttattagaa aaaagaataa
      601 atcacataat gaatgctttt taaaaaagat aatgtttttg gaaaggatta aatatgttaa
      661 gtattattaa ttcaagcaac ttagtaggaa tcgacagctt tttagttaag gttgaagtag
      721 atgtaagcaa tggaatacct agttttaata ttgttggtct tccaggcaaa gaaattaaag
      781 aagcaagaga aagagtaaag tcagcaattc ttaatagtgg atataaattt ccaagcacga
      841 gaatagttgt aaatttatca ccagcagata taaaaaaaga aggagctttt ttagatttat
      901 ctataagtat aggcctttta agagagttaa taaaaaaaga tgaaaattac ataagagaaa
      961 gtatgtttat aggagaatta tcattagatg ggaaaataag aaaagtaaga ggtatattgc
     1021 ccataattat gggtgcaaaa actcagaaca taaaacgtat ttttatacca attgagaaca
     1081 taaaagaaag tctatttgta gatgaaattg atataattcc tattaaaagt ttaaaagaat
     1141 gtgtcgattt tttaaacgaa gagataaaag tcgataaagt aaggataatg tcttttttgg
     1201 atgataagtc aagaaaagag aacgaagaat tagaaaaaga taatagttat atagattgca
     1261 aatatactaa gattaataat gaagaaagta aatatgatga agactttaaa gatgtaaaag
     1321 gaaattattt tgtaaagagg agtgcagaaa ttgctgctgc tggaaatcat aatatgttta
     1381 tgattggacc tccaggctca ggaaaaacta tgattgcaaa aagagtaagg acaattttac
     1441 cagatataag tgtagaagaa atgatagaag taagtaaggt ctatagtata ttgggtatga
     1501 taaatgaaac taaaggaata attgataaaa gaccttttag agcacctcat catacaacta
     1561 caaagcaatc tttaattgga ggaggaatgg atgcaagacc tggagaaatt gctttagcac
     1621 atagaggtat cttattttta gatgagatag ccgaatttga tagaaaaata cttgagactt
     1681 taagacaacc tatagaagat ggatatgtaa atatatccag agtaaagtac agtgcaaaat
     1741 atccttgtag agtactgtta gttgcagcta tgaatccatg tccatgtggt tattatatgt
     1801 cagagacaga gtgtagatgt agaagtaacg aaattgatag atacataaat aaaatatcag
     1861 gtccattgtt agataggttt gatatttttg tagaagttaa ttctataaaa tattcagatt
     1921 ttaattcttt aaaacaagaa gaaagctctc aaaaaataaa gagaagagta gaaaatgcta
     1981 gaaaaataca gataaataga tttaagaaag ataatataaa aaataatagt gaaattaaag
     2041 cttacaattt atttaaatat tgtaaactgg aaaaagaagc ttcaaaaacg gctgagatga
     2101 tatttagtaa atacaaccta agcagtagaa gttatacaaa acttttaaaa atggcaagaa
     2161 caatagcaga tttagaagaa agagatttga taaattcaca gtgtattata gaagcttttt
     2221 catttagaaa ggcttattat agttatttta aatagttttt tgaggtggat tttatggaaa
     2281 aaagagatac ttatttatgg ttaaaatcaa ttggtggaat aacaacaaaa acaattgaaa
     2341 taatagaaaa tgaaatagta aatattgaag atatatttga tttttctgaa aaagaaatat
     2401 ataatttaaa aaatataagc ttgaatatta gaaaaaatat agtaaaatat agaggtcacg
     2461 cttatttaga aaatataaaa gaactacttt acaaaaaagc gattaaatat atttgtaaat
     2521 atgataaaga atatcctgaa aatttaaaga atatatacaa cgctccaaag ttattattct
     2581 acaaaggtga cataggctta gttaataata attttaatat agctatagta ggttcaagaa
     2641 aacctacagc ctatggaatt aattgtgcaa aaacaataag ttgtcaacta tcccaatatg
     2701 gagttaatat agtaagtgga ttagctatag gaatagatgc atactcgcac ataggttgta
     2761 tgagtggaaa atctaaaact atagcagtac ttggttcagg agtagacaat cctcttccaa
     2821 aacaaaattt acatttatca aataaaatat tagaaaatgg gggattatta ttatcagagt
     2881 ataatattaa ctcgacagta gccccgtacc atttttctaa tagaaataga attatcagtg
     2941 gtttaagtga tggagtagtt gttgtagaag cagcaataaa aagtggtgca ctaatcacag
     3001 tagattttgc tcttgaacat ggaaaaaatg tttttgcaat acctggtaat ataaattctc
     3061 aaatgagtag aggttgtcat aaaattataa aagaaggtgc aaaattaatt gaaaatatag
     3121 atgatatttt aaatgaatat aacatattta atattattga taaaaaaata aatcaaaaat
     3181 atgataatat aagtctaaat gccaagtcta agcaaataat tgaagctata aaaagagaag
     3241 gaaacttgca tatagatagt atttgtgatt atactggtat agaaataaag tatgtaaatt
     3301 caataatcaa tgaattagta ctaaatgagt tagtagtaga aatgaataat aaaacgtaca
     3361 gtttaaatgt ataaattaag attaggtagt gggggtgtat ggttttatgg ccaaaacatt
     3421 agtcatcgta gaatcgcctg caaaagctaa aactatagaa aaatttttag ggaaaagtca
     3481 ttacacagtt aaagcatcag ttggtcatgt cagagattta cctaaaagta aattaggtgt
     3541 agatatagaa aataattttg aacctcaata tataaatata agaggaaaag gcgatgtaat
     3601 aaaagaatta aaaaaagaag ctaaaaaatc taaacaagta tatctagcaa ctgaccctga
     3661 tagagaaggt gaagctatat catggcattt agctcatata ctaaatttag atgaatctga
     3721 ggattgtaga atagagttta atgagataac taaggatgca attaaaaaag caattaaaca
     3781 tcctagaaat attgatataa atttggttga tgcacaacag gcaagaagag tgctagacag
     3841 acttttaggt tatcaaataa gtcctatatt atggcaaaag gttagaaaag gtcttagtgc
     3901 aggtagagtg caatctgtta caacaaagtt aatttgtgat agagaaaaag aaataaaggc
     3961 atttattcca aaagaatact ggacaataga tgttgaggct aagacagtaa aagacaaaga
     4021 tataactttg aaattttatg gtaaaaatga tgaaaagatt gaattagaaa atgaagaagc
     4081 tgtaaatgaa atactaaaag aaatggaagg taaagattta aaggttgaga agatagaatc
     4141 taaatcaaga cgtaaatcag ctccaaaacc atttacaaca agtatgcttc aacaagaagg
     4201 agcaaataag ttatttttta ctactaaaaa aacaatgatg atagcacaag aattatatga
     4261 aggtatcgac atagaaggtg aaggtactgt tggtcttata tcatatataa gaactgactc
     4321 taaaagaata tctgatgaag caaaagaaaa agctaagggt tatatcgtaa acaatttagg
     4381 agaaaactac tataaaaaca ttggtgataa aaaagaaaaa aaggatacta aaaaggttca
     4441 agatgcccat gaggcaataa gaccaacctc agttgataga actcctgaga gtataaaagc
     4501 ctcattgagc aaagaccagt acaagttata caatttaatt tggagaagat ttgtagctag
     4561 tcaaatggaa gattcagtgt ttgatatact aaatgtagaa tgtaaaatag gcgatattgt
     4621 atttaaggca actggttcaa aaatgaaatt tgatggttat accaaggtat ataatttttc
     4681 agaaagagaa gacaaaattt taccaagtat agaaaaagga gatatattaa atatagaaaa
     4741 tatattgcca gaccaacatt ttacacaacc accagcaaga tatacagaag ctagtttagt
     4801 aaaaacacta gaagaactag gtataggtag accaagtacg tatgctccaa caatagctac
     4861 tatactgaat agagagtatg ttgaaaagca aggaacaagt ctatgtccaa cagaacttgg
     4921 aataatagtt actgatattt tggaaaataa tttccataaa tttatagatg tagattttac
     4981 tgctgatatg gaaagtaaac ttgatgttat agaagaaggc aatacagatt ggaaaaagat
     5041 tgtatcagaa tcatatgcac cacttaaaga ggctattgaa gaagctcttg aaaatataga
     5101 aaaagtaaat atggatgaag aaactgatga gatttgcgag aactgtggtt ctaatatggt
     5161 tatcaaatat ggtagatttg gaaagtttat ggcttgtaaa aactatcccg attgtaaaac
     5221 aactagacca attatgaata aaattggtgt taaatgtcca aaatgtgaag aaggagatat
     5281 tatacttaga aaatctaaaa gaggtaaagc tttttatggg tgttctaatt atccaaactg
     5341 tgactttgta gcttggaata agccaacagg agagttttgt gagaagtgtg gttcatatat
     5401 ggttgaaaaa gtgactaaat ctgaaacaaa aacagtgtgt tctaataaag aatgtaataa
     5461 agagacaaaa gagcataatg acggaaaaga gtgaaaattg aaaaaaatca cataattctc
     5521 ttgaaaaagg ttccaatcta tgataatctt tcaagtgtaa acaactaata gaaaaataaa
     5581 ttttcattaa atgtgaaaat aatattagaa aatataaaag gttattaaaa ggcctaaatt
     5641 tcatgaggag atgattaaat ggcaagtgaa gtgttacaaa aaacaaggaa aataaataaa
     5701 acattacaaa caagtggtgg aagcagtgtc tcttttgatt tactggccgg agcattgggc
     5761 gacgttttaa gttctaatgt ttatgtagta agtgcaaaag gtaaagtact aggtcttcat
     5821 ttaaatgatg ttcaagacag ttcagttata gaagatgagt atactaagca aaagaaattt
     5881 tcagatgaat atactcaaaa tgtgttaaaa attgatgaaa cattagaaaa tttaaatggt
     5941 gagaagatat tagaaatctt tcctgaagaa catggaagat tacaaaaata tactacagta
     6001 gttccaatat taggaagcgg tcaaagatta ggaacattgg tactttcaag atattcaaat
     6061 tcattcaatg atgatgattt agtaatagct gaatacagtg caactgttgt tggtcttgaa
     6121 atattaagag caataggtga ggaattggaa gaagaaatga gaaagaaagc tgtagttcaa
     6181 atggcaatag gcactctgtc ctactccgag cttgaagcag ttgaacatat ttttgctgaa
     6241 ttggatggaa aagaaggttt acttgtagca agtaagatag ctgatagagt tggtataact
     6301 aggtctgtaa tagtaaatgc acttagaaaa tttgagagtg caggtgtgat agaatcaaga
     6361 tcattaggta tgaaaggtac tcatataaga atacttaatg acaaacttac agatgaatta
     6421 aaaaaattaa aaaacaatca ataatctata ttttataggt ttagattaga aaaggtatct
     6481 atttcaaata gatacctttt ttagacgaat tattaaggta atgataagta tattaaaaag
     6541 gagtgagaaa atgcctttag cagataaaat aagaccaaaa acaatgaatg atgtaatagg
     6601 tcaaagtcat ataataggta atggaaaaat attgtctaag atattacaat ctaatttttt
     6661 accaaatatg atattttttg gtcctcctgg tgttggtaaa actacagtag cggaaataat
     6721 agcagaaaga agcaataaaa gcttctataa aataaatgct actaattcat ctttagaaga
     6781 tataaaaaag gtgatatcag agcttggaag tataaataat gtaaatggag tcctattata
     6841 tatagatgaa atacaaagtt ttaataaaaa acagcaacaa tcaatattag aatttatgga
     6901 aaatgggagt ataactttga ttgctagcac tacagaaaat ccatatcatt atgtatataa
     6961 agctctttta agtaggtcta ctgtatttga atttaaacct ttagaaaaac ttgatataga
     7021 aaaaggactg aaaagagctg ttgaagtatt aaatgaagat agctatatgg atattgagtg
     7081 taataatgat gctattgaag atatagcaat tctttctgat ggagatatga gaagagcctt
     7141 aaatattttg gaagtggtag tctattcaac taaaccaaat aaggataata taacttatat
     7201 agattcagat gtaataaggg catctacatt taataaaatt ataaactatg acaaaaatgg
     7261 tgatagtcat tatgatattt taagtgcatt tcaaaaatct ataagaggaa gtgacccaca
     7321 ggcatctata cactatctag caagacttat aaaaggtggg gatttgataa gtatatgtag
     7381 gagactttta gtaatagcta gtgaagatat aggtttagct tatccaaatg ctattgtaat
     7441 tgtaaaggca tgtgtagact cagctatgca acttggattt ccagaagcga aaataccatt
     7501 agcagaagct actattcttt tggctacatc tccaaagtct aattctgcat gtatagcaat
     7561 aatgaaagct atggatgaac tggataaaga gtttgttaag gatataccaa atagtataaa
     7621 ggatgctcac tattctggtt ctaaagaaat aggtaggggt tgtggataca aatatcctca
     7681 taattttaaa aatcattatg taaagcaaca gtatttacca gatagcataa aagatagtat
     7741 ttattacata ccacaagaaa ataaaactga gaaaaatata aaaaaatatt tagaatatct
     7801 aaatagtgat ttttagactg aactttatgt atcgggggta gatattatga ttataaaact
     7861 taactcaact tatcacagta gagttatgaa atacttaaaa aaagaacctg agtacaattt
     7921 gtttataata ggggatattg aaagatatgg atatggaaat aattttttaa atatatgggc
     7981 agatgtgggt gaacatggtg aaataaaggc aatattactt aaatattttg aatttatgat
     8041 gttttattct gatggagagt atgatgttga aggtttttat aatctacttc gtaatactaa
     8101 ttacgaagaa atatctggga aaatatgtgc tgtagatgct ttagcaaaga gattaggact
     8161 taataattta aaggtagttg atttttgcaa acttcaaact aaaaagtttt tgatagataa
     8221 taattgtaat gcaaaggtta agagaattag gcttggaaat ctaaaaaaga cagtaaaact
     8281 ttatgactta atagatgaat ttcacagtac aactttagag aatttaaaaa atggactaag
     8341 gacaggtaga ggatattgta tagagattaa caaacaggtg gtgtcaatgg caaagagtac
     8401 ttcagaaaat aggactcatg caatgataat tggagtagga acacatccta aatatagagc
     8461 taaaggactt gctacaaaat gcctaattaa actatgttca gagttactga gagaaaacaa
     8521 aattccttgt ttgttttatg ataatgaaga ggctggaaag atatataaaa aactaggatt
     8581 tgaaaacatt ggaaaatggg gaatttattc aaaataaaaa attaaaatta atcttgacaa
     8641 aattagtagg gtataataaa attatcttga aagtaattcc tactaaaaca gtatgaatta
     8701 gatttctata tagaggtgat aatatatgaa gttgtctact aaaggtagat atggactaaa
     8761 agcaatgttt gaattagcat taaatcaaga caatggacca gtatcattaa aatttattgc
     8821 taagaagcaa aaaatatcag atcagtactt agaacaaata ttttcatctt taaaaaaatc
     8881 gggtctagta aaaagtgtga gaggtgctca aggaggttat ttgctttcaa agaatgctga
     8941 agacataaca gtaggagata tacttgtagt attagaagga ccagttgcat tatctgactg
     9001 tgtactagat gaagatgtat gtgaaaactc aaacatgtgt gtaacaaaaa tagtttggga
     9061 aaagatgaaa aagggtatag aagatgttat agattcaatt actcttaaag atatgataaa
     9121 tgactataac aaaaataaat tagaaaatga tataacaaat attaaaaaat agatttggag
     9181 atgaatgtaa tggaaaaaag aagattgtat atggattatt ctgcaacaac acctattaaa
     9241 aaagaagtat tggatgcaat gatgccatac ctaacagatt attttggtaa tgcatctagt
     9301 tttcacacat tcggaagaga agcaaaagat gctttggata aagcaagaga acaagtagca
     9361 gcccttataa atgctgaacc tagtgaaata tattttacag caggtggttc agaaagtgat
     9421 aactggacac tagagggtgt agcttatgct aataaaaaca aaggtaatca tataataact
     9481 tcaaaaatag agcaccatgc aatacttcat acatgtgaat acttagcaaa acatcatgga
     9541 tttgaaataa cttatttaga tgttgatagc gaaggaaaag tagacttaaa gcaactagaa
     9601 gattcaataa aagatactac tatacttata agtataatgt ttgcaaataa tgaaatagga
     9661 actatacagc ctattaaaga aatatccgaa atagctaaaa aacataagat attattccac
     9721 actgatgcag tacaagctac aggtaatatt ccagtagatg ttaaagaatt aggaatagat
     9781 ttgatgagta tgtcttcaca taaaatatat ggaccaaaag gtgtaggagc tttatacata
     9841 agaaaaggtg taagacttca taactttgtc catggtggag cacaagaaaa aagcaaaaga
     9901 gctggtacag aaaatatacc tgcaatagtt ggatatggaa aagcagcaga attagctaag
     9961 gaaaatatgc aaaatcatgt agaaacttta actcgtctta gaaataagtt aatagatggt
    10021 gtgttagaaa gaatacctta cacaagagta aatggtagct tagaaaatag attgccagga
    10081 aatgcaaact ttgcattcca atttatagaa ggtgaaggaa tacttttatt attagatatg
    10141 ttaggaattg ctggttcaag tggttctgca tgtacttctg gttcattaga cccttcacat
    10201 gtacttcttg caataggttt accacatgaa atagcacatg gttcattaag acttacagtt
    10261 ggagatttta caacagatga tgatatagat tatatactag agaacttacc aaaagtaata
    10321 gaaagactta gaagcatgtc accactttat gatgatgcga agaaacaagg tttagtaaaa
    10381 tagacataaa gataaagcat aaaaaataga cataaattga gtaggaggaa aagataatgc
    10441 aatatagtga taaagttatg gaacatttta tgaatccaag aaatatggga gaaattgata
    10501 atgcaagtgg tgtaggtgaa gttggaaacc ctacatgtgg agatataatg aagatatttt
    10561 tagacataga tggagatgta ataaaggatg ttaagtttaa aacatttggc tgtggttcag
    10621 ctatagcaag ttcttctatg gcaactgaaa tgataaaagg aaaaactata aaagatgcat
    10681 tagaacttac aaataaagct gttgcagaag ctttggatgg tttaccacca gtaaagatgc
    10741 attgttcagt tttagctgaa caagctgtaa aagcagctct tatagattat gctcaaaaga
    10801 acaatatcca tataccagaa ttggatggat atgttataga tgatgctcat gaccatgatg
    10861 ttgaagaaga agaataaaaa taagtgattg tgttatgaga agtttacaat gattaaaaag
    10921 aagtactaat gatataatta ggacataaaa aatagctgtt tttaaaatag ctatttttta
    10981 ttgcctaaat taagtatttt aacttgattt agaggttaat tagttaacaa aataatccat
    11041 aagattcaaa taataaagat gattattttt ttagaaaata gaacaagcta tattttaaat
    11101 aagtacaata cttgctctat atggtataat aataagtaat gcaaaagatg aggtgaaatt
    11161 tgtttatgaa taaaaaagta atgataggta tgagtggagg agttgatagc tctgtagctg
    11221 catatctttt aaaacaacaa ggatatgatg tcattggtgt tactatgaag ttgtggcaag
    11281 atgatgatgt tgttgaaatt gaaggtggtt gttgctcttt atctgctgtt gaagatgcta
    11341 gaagggtagc aaataaaata ggtataccat tttatgtttt aaattttaga gaagtgttta
    11401 aagaaaaagt aatagattat tttatagatg aatatttgga aggaaaaact cctaatccct
    11461 gtatagcttg taataaacat ataaaatttg atgattttta taagaaggct agacaaatag
    11521 gttgtgatta tgtagctaca ggtcactatg caaagataga aaaggatgaa agtacaggaa
    11581 gatatttgct aaaaaaatct gttacagata aaaaagacca aacttatgca ctttataatc
    11641 ttacacaaga gcaactagag catactttat tgccaatagg tgactatgaa aaagatagag
    11701 taagagaaat tgctaaggaa atgggtatgg cagtacacaa taagcctgat agtcaagaaa
    11761 tttgttttgt taaagataat gactatgcaa attatgtaaa aaaacattct aaaaagcgta
    11821 ttgaagaagg tttttttgta gatactaaag gcaatatact tggaaaacat aagggtatat
    11881 tatattatac tataggccaa agaaaagggc ttggaattac ttttggaaaa ccaatgtttg
    11941 taatagacat aaatccaata aataatacca tagttcttgg agacaatgaa gatttattta
    12001 aaaaggaact tattgctaaa gatgttaact ttatatcaat agatacgtta gaagaaccct
    12061 taagagttca agccaaaata agatattcag caaaaacatc accagctact attcatagag
    12121 taggagaaga tactattaaa atagtttttg atgaagctca aagagctatt actaaaggtc
    12181 aatctgtagt aatgtatgat ggagatattg tagtaggtgg aggtataatc gagaaaagtt
    12241 tataaaaaca tctaaaaaat taaaaaaata agcaataaag tatattaaga gtaaataaag
    12301 tttataaaat aaatttattg tatatgaaaa tatagtattg attaattttg taaaatttag
    12361 taatataatt agtatattaa ttaaaaaaca aatacagtga taaggaatag taaaatattt
    12421 gatgtcttac agagagaggg aaatgctgga agcccttaga atatgatatt tgaagcagcc
    12481 ttttagtagc aatcttgaac aatttatctt ttataaagaa gatttaagta gagattgacg
    12541 gtagttacgt tatagctaag gttttccgaa gatatcatac tttgtatgat aattagggtg
    12601 gtaccgcgat tgtcctcgcc cctataaatt tatttatagg gacgaggttt tttatttaaa
    12661 cgaaaaaata aaatttatta ggaggaacat gatatggaaa agatgggatt aaatgaaata
    12721 agaagtaagt ttttagaatt ttttgagtca aaaggtcatt atgtagcaaa tagttattct
    12781 ttagtaccaa acaatgataa gagtttgtta cttataaact caggtatggc acctttgaaa
    12841 aactactttt caggtgtaga agtacctcca agtgtgagaa tgtgtacatc tcaaaaatgt
    12901 ataagaacag gagatataga aaatgtaggt ataactgcaa gacatgctac attctttgaa
    12961 atgatgggta atttctcatt tggagattac tttaaaagag agtcaataaa atggggatgg
    13021 gaatttgtta cagaatggct aaatatacca gaagataaga tatgggtgac tgtatatgaa
    13081 gaagatgatg attcttatga tatatgggca aaagaaatga atttcccaga agaaagaatg
    13141 gttagacttg gaaaagatga taatttctgg gaaataggta caggtccttg tggtccttgt
    13201 tcagaaatat attttgatag aggagaagaa tatggatgtg acaatccaga ctgtaaacca
    13261 ggctgtgatt gtgataggta tttagaattt tggaaccatg tatttactca atttgataga
    13321 gatgaagaag gaaattatag tctattagaa aacaagaata tagatacagg tatgggtctt
    13381 gaaagaatgg gatgtattat gcaaggcgta gacactatct ttgaagttga tacaattaaa
    13441 tctatactag aagcagttga gaagttaaca ggagtaaaat atggagagaa tccaaaaaat
    13501 gatatatcaa taagaataat aactgaccat ataagagctg taacattctt agttagtgat
    13561 ggagtgcttc catcaaatga aggaagagga tatgttctta gaagactcct tagacgtgct
    13621 gctcgtcatg gtaagttatt aggagtaaaa gaattatttt tacagaaact aatagatgaa
    13681 gttataaaag taaatgataa agcatatcca gttttagttg aaaaagaaag ttatattaaa
    13741 aaagtagttg gaatagaaga agaaaaattc aatgaaacta tagaccaagg aacagaaata
    13801 ttgaactctt atatagaagt attaaaaaat gaaggaaaga ctgtcctaag tggtcaagaa
    13861 gcttttaaac tatatgatac ttatggcttc cctatagact taactaaaga aatattagaa
    13921 gaagaacatc tatctgtaga tgaagaagct tttaatgaag aaatggaaaa gcaaaaagaa
    13981 agagctagaa atgctagagg aaatatggat ggagaaagtt ggaaggaaga ccctctatca
    14041 aaattagaat ctacagttga cagtacattt aatgggtata gtgaaattta tggtgaagga
    14101 actattgaag ctatagttaa agatgatgag ctagttcaaa gtgcagaaga aggcgataag
    14161 gtatctatag tactagataa cactactttc tatcctgaag gtggaggaca agttggagat
    14221 tgtggattga taactaatga aaacttagtt ttagaagtat taaatactaa aaaaggtgct
    14281 aataatagta taaaacatat aggtataata aaatcaggaa gaataagtaa tggggacaaa
    14341 gtgaagacac tcgtagatag agaaactaga atgtcagctg caagaaatca cagtgctaca
    14401 catttacttc ataaggcatt aagagaagta ttaggagaac atgttaatca agctggttct
    14461 ttagtaactc cagaaagact tagatttgat attactcact ttgaagcgat atcaaatgaa
    14521 gaattaaaag taatagaaga aaaagtaaat aatgttattt tatcatcttt agacataaaa
    14581 tgtgatatta tgaatataaa ggaagctaaa gaaaaaggag ctacagcact atttggtgaa
    14641 aaatatggtg atgaggtaag agtagtttct atgggagatt attctacaga actttgtgga
    14701 ggaactcact tgacaaatac ttctcaagta ggaatgttca aaatattatc tgaaggtgga
    14761 gtagcagcag gtgtaagaag aatagaagca ataacaggaa aagctgttta tgaataccta
    14821 aaagaaagag atggaattat ctctgaagtt tgtgtaaact taaaatctaa agaagataat
    14881 ttaattcaaa gaataagttc tttattagaa gaaaataaaa acttatctaa agaattgcat
    14941 gatatgaaag ctaagatgag tttacaatca gttgattcta tttttgattc taaagttgaa
    15001 gtaaatggag ttaatttgat aactaataaa tttgaaggta tggatatgga tacactaaga
    15061 gaaacagcag ataatctaag agataagctt ggttctggtg tagtagtact tgcaaatgta
    15121 gtagatgata aagttaactt tgtagttact gccactaaag atgtattaga taagggaata
    15181 cattcaggta atattgttag agaagttgca aaaatagctg gaggaaaagg tggaggaaga
    15241 cctaatatgg cacaagcagg agctagtgat gtatctaagg ttgaccaagc tttaagctat
    15301 gcaagtgagg ttattaagac acaagttaaa taacattcac tcaaggaggt ttttagtttg
    15361 gaaaaagatt tagactacac tatgaaattt gaaggaatac cagaagatag gatgagtgta
    15421 ggagatacta ttgattttgt atataaagca ttagttgaaa aaggatataa ccctataaat
    15481 caaattatag ggtatctcct ttctggtgat tctagctata taactagtca taaaaatgca
    15541 agagctataa ttaaaaaatt tgaaagagat gagatacttg aagaagtaat cacgcactat
    15601 ctgaatagaa agtaggtcaa ataaatgaga aaaaagatta tgtcgttgtt tgcaatagtc
    15661 attatgattt ttgtatcaac tgctagctat ggttttgctg aagaaaaagg caaggtcata
    15721 tttattgata tgaataggac caatcttgga agtatgatga atattccaat cttgaaaaat
    15781 gagatagaaa agagaggcta tgtagctctc atgaatataa gaggggatca aggtactgat
    15841 gatagaaggt cttatgcaag tatgggtgca ggaacaagag ctagtgtaac tactgaagac
    15901 tatatcaatt ttcaaaaagt agaccaagat actgctaaag cttttgaagt cgcaacaggt
    15961 caaaaagcta aagaaataaa tgatttaggc attaatagat ctataaatga aaatcttgag
    16021 tatggagaat atggagcaaa tttagggtca ttaggaaaaa ctttatcaga aaatggttta
    16081 aagacatctg taattggaaa tgctgatata attgaaaata atcaattagt taaaaacaga
    16141 aacttatgtt tagtagctat ggaccaatat ggaaggattg attcaggtaa tattgataat
    16201 attaataaga aagattctaa aatgccattt ggaatatcta cagattatga taaattacta
    16261 gatgaaacta aaaaatccta taaaaatagt gatgctatat ttgtagaact tggagataca
    16321 tatagattag atatgtatag attaaatcta aatgataata cttatgatat aatgaagaaa
    16381 aatattgtaa aaaatataaa taaatatctt gaacaagtat tttctatggt tggagaaaat
    16441 gacactgttt atatagcaag tgcatttcca agtgacctag attataagaa taagagaaga
    16501 ctttctccaa taataaaatt tagtggagaa gggaaaggtc tactggaatc tgccacaaca
    16561 agaaaggctg gaattgttgc caatttagat gttggagtag atatactagg tacatttggt
    16621 cttgaaaata aagagatggt aggtagaagt tatactttag taaataaaga ggacaatatt
    16681 agctatctaa aatcacttta tgaaaggata gtgtctatat cttcaataag gtcaactatt
    16741 ataaatggct ttgtaggcgt aatatctgct tcttgggtaa ttgccatgtt tgcgattttc
    16801 tttagaaatt atatgccaca taaagagcgt gtattcaaag tacttaagga gtttataaaa
    16861 ttaggcataa taatgccact atcatttatg ttagctcctt tgatgaattt taaaactcca
    16921 atttcaatat ctttgggaat atttgcaact acgctaatat tatacttgtt aggtagaaaa
    16981 ttatttaagg acaatgatat aaagcacatg ggattttttg caacacttac tattttagtc
    17041 atagttatag attctgtatt tggaacatac ttaatgcaaa ataacataat gagttatgat
    17101 gctgtcatag gagctaggta ctatggtgta ggaaatgaat atgaaggtgt aactatagca
    17161 agtgcagtat ttggcttagc agtattatta cattataaga aggtatctaa attacttgtt
    17221 gtaatttttt cattaataat acttataact agtgcatatc cttcaatggg tgcaaatgtt
    17281 ggtggagcta tatcagaatg tgtagcatat ttgttgttta taatgcttat atatgacatt
    17341 aaaatagatt ttaagaaagc tgtattatta gctgtttcag ctgtagttgt cgtttttgca
    17401 tttgcagctt tagatatact ttcaggtagt gaatctcatt taggtatgtt tgtacagaga
    17461 attttcctaa atggaccagg agagatatta caaacatttg gaagaaaaat tcaaatgaat
    17521 atgcaactag cacagacaag tgtatgggta aatattttac ttgtaggaat tggagtaatt
    17581 gcagtgctta tatttaaacc atctagacac tttagaaaga taatgaataa ttttccaatc
    17641 ttgtttaaag ggtttattgc atctatggtt ggttgtataa ttacactttt agttaatgat
    17701 tctggaatag ttgctgcatc tacagcatct atatatatac ttataccact aataataata
    17761 agtataaata tgataatatt tgaaaaaaat aactaaaaaa atttttactg ttcatatagg
    17821 atttaaataa aaaatgcaat atgattatta atgtaaactg gatgcttaca taaaaaatca
    17881 tattgcaatt tttatgattg attatgtaaa attatatgaa gcagtaagcc tgtgacctta
    17941 gttatgagag gttcagaata tgtatttaat ctatagaata taaatagata aaggaggaac
    18001 tatgttagat ggaagaataa tgggtcttga tgttggtgat aaaactattg gagttgctgt
    18061 gagtgatttg atgggattaa ctgcacaagg tgtaaaaaca ataaaaagag ttggcaagaa
    18121 aaaagatatt gaagagctaa aagcaataat aaaagaaaaa caagtaaata aaattgtatc
    18181 aggattacca aaaaatatga atggaacttt aggtccacaa ggagaaaaag taatcaagtt
    18241 ttgtgaattg gtaaaagcag aaacaggtat tgatgtagag ttttgggatg agaggctttc
    18301 tacagtagcg gcagagaggt ctttattaga agcagatgtt agtagacaaa aaagaaaaaa
    18361 ggttatagat atgttggcag cagtaataat tttgcaagga tatttagatt ttaaaataaa
    18421 ttcataaaag aactataaaa ttgaatactt atgatatata atataaataa taaaaataaa
    18481 aataaatttg aggtgaatga cattatggaa gaaaatataa taaatttaat agacgaaaat
    18541 ggcgttgaaa gccaatttga aataatatta acattagaag cagaaggaaa agagtatgca
    18601 atattaatgc ctttagatga tgaagaagct gaagaagctt taatctttag aattgatgag
    18661 gatgaagaag gtgaaatctt aatacctttg gaaagtgatg aagagtatga aactgttgta
    18721 gctgtgtata ctgctataat ggaagaagaa ggtttaaatt acgacgaaga tgaatcaaat
    18781 ggacttaact agataagtaa ttaagtttgt atataaaaat cagatattta ttatatttgc
    18841 tttaattaaa cattattaat tgtaaaagca tttatagtat atatctgttt ttttgttttt
    18901 tattaaaaat accaaaaaat ttatgaattt tggtgaataa tatatctttt atgggtatat
    18961 atttatatta tgagaataaa ttaaatttat ttaaatgtct tgacaaaaaa tgaattatca
    19021 attacaatat tatttaataa agattatcaa ttgaaaatag taagggtgat gttatggcaa
    19081 atacaatgga tttattaaaa gacaagttaa aagaaactgg ttttaagatt acgccacaaa
    19141 gaagagcaat agttgaaata ttgcttaaac atgaccattc acatctaagt agtgaggaaa
    19201 tctatgattt agttagggtt gactgtcctg aaataggctt agctactgta tatagaacaa
    19261 tgcagctatt ggatgaaata ggattaatat caaaactaaa cttagacgat ggatgtataa
    19321 gatatgagat tagtctacat aaagaagact gtcataatca tcatcatctt atttgtaaaa
    19381 attgtggaaa gataatggaa gctaaagaag accttttaga caatatagaa aaagagatac
    19441 aatcattgta caaatttaaa atacttgatc atgatgttaa attttatgga ctatgtgacg
    19501 agtgtaatgg agtaagcgat tcagaagaat aaacttctat tgcttaatgt cgatggaaga
    19561 atagtataga acttttatat tctatgctat tcttccattt tttattataa accatctata
    19621 aaatgcaaat atacagtttt caagatgctt atagttttaa tattgaaaat ataattaatc
    19681 ctataagtac aaaaatgaat gatttttcta tgaaattact aaatggtcct ccaacagtta
    19741 aaaaacctaa agaaattttc ttttcgctta gcggataaaa aattggtatt ccttgctttg
    19801 taaataaatc tcctaaaaac atatgacttg cataaccaat cgtaccataa aaagataaat
    19861 tattaatatt agtgattaat tctatttgtt ttagtaaaaa atatactatt atagttgcaa
    19921 atatgctatg agaaaaactt ctgtgcttta accagggaaa aattgacaac attagacaaa
    19981 aaattgtaaa atatatttca acttctatga tgtataagca tatagctaat agtataaata
    20041 ttaatgatag aaaaactttt cttagaaatg tgtgggttag ttttgcctca ataattataa
    20101 ttgcaacaaa tgtaattatt gcgcttaaat aaaaattatc atttatattt attgaaataa
    20161 agaaaataat aatattaaca gcatatataa aaattctata tatgtatttg gatacattgt
    20221 tttttagaat gaaatttgag accatagaat tagatttatc caaatcgggt agtacagcaa
    20281 aagtagctga aactaaaata tcaactaaag atataggaat tttaaatatt aaagatgttt
    20341 ggatagctgt taagattcct attgcacaat gagttttacc acgcataaat cctcctaaat
    20401 tatccgatat taaatcatat aatatttaga ggatatcaaa ataaaatagg tttgtcaaaa
    20461 taatacgtta aatgtatttt aaatatagct taaaatatga tacgaattat ggtacaataa
    20521 gaataactgg tagcgagtgc gtgatttgat attatttatg attgtagaac gtgttaaaaa
    20581 attaaataga cagaaggaga tgatgcaatt gttcaaaaag aacaccaaca agataaaggt
    20641 aatggccttg ggtggactta acgaagttgg aaaaaatatg actgttgttg agtataaaga
    20701 tgaaataatt gttatagatg ctggacttag ttttccagag gatgaaatgt taggagtaga
    20761 tatagttata ccagatataa cttatttagt aaaaaatagg gataaaataa agggtatatt
    20821 tattacacat ggacatgaag accatatagg agctcttcca tatatattaa agaaaataaa
    20881 tgtacctgta tatggggcaa gacttagtat aggtcttata caagtaaaac ttaaagaaca
    20941 taaaatgaat aatgttaagt taaatgttat aggtcctaga caagttataa agcttgataa
    21001 tatggaagta gagtttttga aaaataacca tagtatacca gatgcttact ctattgcaat
    21061 acacacagac caaggtataa tataccacac aggagatttt aagattgact tgactcctat
    21121 tgatggagat gttatggata tgcatagaat ctgtgagttg agcaaaaaag gagttctttt
    21181 aatgctggca gatagtacta atgcagaaaa accaggattt actatgtctg aaaagactgt
    21241 tggggtaggt cttgacgaat tatttgcaaa aggaaatggc agaagaatta tagttgctac
    21301 ctttgcatcc aacatacaca gattacaaca aataataaat actgctgaaa agtttaatag
    21361 aaaagttgct atatcaggtc gctcaatggt caatgtagtt ggagttgcaa aagaattagg
    21421 atacctagat atttctgatg acatgcttat agatttaaat gatatatgta agtatgaaga
    21481 tagtgaatta gtgataataa caactggttc acaaggtgaa cctatgtcag ctcttgctag
    21541 aatggctttt tcagagcaca aaaaagtaga aataaagagt ggcgatttag ttataatatc
    21601 tgctcatcca ataccaggaa atgaaaaatt aatatcaaga gtaatcaatt tcttatttga
    21661 aaaaggagca gaagtagtat acagtgatat agctgatata cacgtttctg gacatgcctg
    21721 tcaagaagaa ttaaaactta tacatgcatt agttagacca aagtttttta tgccagctca
    21781 tggtgaatat agaatgttaa aaagacatgc tgaaatagct gaacagcttg gtatggataa
    21841 agaaaatatt tttgtcatgc aaacaggaga tgttttagaa ttagataaaa attctgctaa
    21901 ggtagcaaac cgtatacaaa caggaaacat attagttgat ggtctaggag taggcgatgt
    21961 aggtaatata gttttaaggg atagaaagca tttatcagaa gatggtctta tgatagttgt
    22021 agttactatt tccaaggatg agggtaaggt cttagcaggt ccagatataa tttccagagg
    22081 attcgtatat gtaagagagt cagaagattt aatggatgga gcaaaggata taattaagaa
    22141 tgttctaaat gaatgtgaag agaaaaatat taaagaatgg gcttatttaa agaataatat
    22201 taaagaaaac ctaaaagaat atttatatca aaaaactaaa cgaaatccaa tgatacttcc
    22261 tatcataatg gaagtataat ataaaaaaac tataaatttg aaaattagac ctcttgtaat
    22321 ttttaaagta atatcaaaaa tccaattgat attatcttaa gaattacaag aggtcttttt
    22381 aatactcatt ttaaaaaaat atatattaca taaaatacca attataacaa tcttttatta
    22441 gtaaaaacta cttatatata aattttactg ataggaggat tcttatggat gatgtttcaa
    22501 gacaaaatgc tataaaagct ttaaaacaaa caaaaatgga aatagcagga gagtatggaa
    22561 tgaattatga agatgctttt gaaataatag aaaatgcgtc taataaaggt gttttagaag
    22621 gttactttaa aaagcttgaa aagaaaaaaa atttaggtca aggtatatct aggcatttag
    22681 aataaaaaaa gttaaatgtg agaaaataag tttaaataaa ctttaagagg tgacatcgat
    22741 gaaaaaatta gctagtactg ctttggctat attaattgcg cttactcctt taagcttttc
    22801 ttttgcaaat aataaggaaa atgctgatgc caaccaacta aatatatctt caaaatcagc
    22861 aattttgatg gatgttggta gtggacaaat tttatatgaa aaggatgctc ataaaaagct
    22921 tcctcctgct agtgtaacta aggtaatgac tatgctactt atagtagaag ctttggattc
    22981 aggcaagatt aagcttgatg atgaagttca agtaagtgaa actgcatcta gtatgggtgg
    23041 tagtcaaata ttcctagagc caggagaaac tcaaaaggtt gatacattat taaaaggtat
    23101 agctgttgct tctgcaaatg atgcttgtgt tgctatggct gaacacttag ctggaagtgt
    23161 tgaaggattt gttgatagaa tgaatgctaa agcaaaagag ttaaatatga atgatactca
    23221 ttttgcaaat acaaatggac ttccagttgc aaaccactat acatctgctc atgatatagc
    23281 tttaatgtct agagaactat taaaacacga aatgataagt aaatacttaa ctacatggat
    23341 ggataaggtt gtagtaggga agaaacaagt tacagtaggt cttgcaaata ctaataaact
    23401 tataaagcat tatcaaggag caactggtgt taaaactggt tttactcaag aagcaaaata
    23461 ctgtttatca gcatctgcaa agagaggtaa tactcatcta gttgctgcaa ctttaggagc
    23521 agagacttct ccagaaagat ttaatgatgc ctctagcctt ttaaattatg gatttgctaa
    23581 ttatgaaagt gttaagttat gtagtaaagg tgataatata gcaacactta cattggataa
    23641 ggctgatgaa aacaaagtta aattggttgc taaagaagat ttaaatgctc ttattaaaaa
    23701 aggaagttct aaagaatttg agaagaaaat tgaaatagta aaaaatccta agatgccaat
    23761 taaaaaggga actgttttag gaaaaataaa gatatgtaaa gataaaaaag taataggcga
    23821 agtggaattg ataaatacta aggatataaa taaggcaagc tatctacaaa tgcttcaaag
    23881 aataattgat aatatgatat aaataattat cagatgaaat ataattataa tacacaaaga
    23941 tatgattata atacacataa aaaataccct tatgtaatta tttgccatag gggtattttt
    24001 atttaccata tagaagtatt ttataatctt atgattttta tagaaatata gaaaagttta
    24061 tgtatcttaa aatatcattt gatttaatat catatatagg gttgtttaac aattatctaa
    24121 gtaaaggcat actaccagta agtttattca ctttcttttt attcccataa attgctatcc
    24181 caaaatactg taactcatct tctggaacac tagcaatctt ttgtgtaaat tcttcataaa
    24241 gattgcaact ttgtgctaca tctgaaaaat ctacaacagt taaatcgtta aattccaaag
    24301 tatataattt tttacgtaaa tcttttatta tttctttgtt tccttttaaa ataggtacag
    24361 gaatgtcaat aattcctaag tggctattac ctgttttgtc aattacatct gggccaacag
    24421 tttctggggc atgttttcct agtgtaattc ccattatagc tgctgtattg gatataattc
    24481 ccattggtaa attttcgtct attaccatga cacattttga atttttagtt tccattttta
    24541 tttctccttt ttaccttatt taaagttgtt tgaatcatca tttataaata tattttgata
    24601 ctgctttggt gtaagaccaa taaaattctt aaaaaagttt gtaaagtgac tttggtctac
    24661 aaatcctgtt tgtatcgcag catctatagg ttctacgcct ttttctaaaa gctttttagc
    24721 tttgtttacc cgtatatttt ctaggtatct atatggtgta atacctttta atttcgtaaa
    24781 attacgaagt agagagtact tattcatacc tgctatagtg cttaattcgt caagtactat
    24841 atgttcagca taattatttt ctagataatc acatactgct tgaatttcta tatttgtatt
    24901 ttccaagtta gattgcaaat ttggctctgt atgttcttca attaattgtt caattaggaa
    24961 gaaaaaaagc tcttcctttt taaaatccaa ttcttcttcc atgattatat agtgtaattc
    25021 ttgtagtaga ggtactagct cactgcgaaa tataacaggt tgattaaatt ttggtaaata
    25081 atttttacct gtaatttcaa agacagtttt tttcattatt tctggcttaa tattaataca
    25141 tctgtaatca agtactttat catctatttg ttcacaagta tgactatcaa aagggttaaa
    25201 aagtagcaaa tctccagtac ttgtggtata ctctttattt ttacaagata aatatctttg
    25261 acctttttct ataaaaccaa ttacataatg ttcatgaaaa tggtttggga atttttgcat
    25321 tatgccttta aatctatatg cttcaatttt taattcccta tcaaagcata ctgtgcgttc
    25381 ttcttgtctc acttaggtaa cctcctttca tgttatacaa agtgtaacac atattttttt
    25441 atacttcttg tataatattg actaagagac aataatgtca attcatgtca atataatatt
    25501 acacttacag taggtgtaat tattatattt tcacaatgaa tatgatataa taataaaagt
    25561 tataaaataa aaagcaaatt ttaattttat gcctagaagt taaaattatt ataatgtaaa
    25621 ataattttga ttataagcta tttaattgtt ataataatat taaaaattta atttaggaga
    25681 tgtacattaa tgttaagtat gaatatagga gaaatagtag caagtttagt tggtatagct
    25741 atggcaatat caatacatga gtttggccat gcttattcag ctcacttatt gggagatgat
    25801 acagctaaag cttatggtag aatgacatta aatcctgtaa gacatgttga cccaataggt
    25861 cttttggcta tgtttatcct taagataggg tgggcaaagc cagtaccagt aaatccaaat
    25921 aactttaaaa actataagat aggaaacctg atagtttcac ttgcaggcgt tttttgtaat
    25981 gttttgactg ctatactgtg tgtaattatc aataagtatg tgcatatgta tgctataaat
    26041 acaatagctg aatatgtgtt tttatatagt attggatttg cagcttttaa cttacttccg
    26101 atacctccac ttgatggatg gggagttata tctacatttg taccatataa atggaatgaa
    26161 tatttgtata aatttgaaag catgagttat ataattttat taattgcttt atttactggt
    26221 atgtatagaa taatattaac accaatatat gcagtgttgc taaaaatagt atatttattt
    26281 gtttaatata ataaacagat taaagaggga ttgatatgaa gtataatata caattacaag
    26341 tctatgaggg accattagat ttactttatg atttaataac aaagcataaa atagatataa
    26401 aggatatatc cataatagat ataactaagc agtatctaaa ttatttgaaa atgctagata
    26461 aaatggattt agagataact agtgaattta taactatggc atcaaaactt ttagagataa
    26521 agtctaaata tctgctttat aagcaaaaag atgaagaaga agacccaaga atagaactta
    26581 tggaaaagtt agaagaatat agaaagttta aggtagcatc acaagatata aaagaaaata
    26641 taacttatgt taatgagaga ttttatagaa ataaagaaga gataattatt gatgacaatg
    26701 tggatttaga agatatatca atagaagcaa taaaaaatat acttccatac atatttaaag
    26761 ttaaaacatc acagatagaa aatgctaatg atgagaaact agataaaata gtaaggaaga
    26821 aaataatatc tgtagaagaa aaaatattat atataagaga tattataaaa gagaagattg
    26881 aagttacttt tacaaatatt ataaaatcat atgaaaatga tgaaataata gctacttttt
    26941 tatctatatt agagcttata aaagaaaaag aaatagtcgt agtacaagat atattttttg
    27001 atgatattct aataagaaaa agttcggagt gttaaaatga aaagagaaga tataaagtat
    27061 attatagaat ctgttatgtt tgcatatggt gaaccaataa gtataaaaga attaaactat
    27121 ataataaata aagaactttc atcaaaagaa atagagatta tgttaaattt acttattgag
    27181 gagtacagag aacaaaatag aggtattcaa ataatcaagt tagagaataa gtaccaaatg
    27241 tgtacaaata aagactatgc agaatatata aagaagatta ttgaaccaaa gaaaaagaaa
    27301 agtttgagcc aagctacatt agaaactctt actataattg cctacaaaca accaataaca
    27361 aaggtagaaa tagaggatat aagaggagta aaatgtgata aggtattaca aactttgttt
    27421 gaaaatgaac ttataagaga agctggtaga ttgaataaga tagggaaacc aataatttat
    27481 aaaacaacag atgaattttt aaagttgttg aatatagaaa gtcttgaaga attgccacct
    27541 atagaaaatt atcaagaagt ggcaacaaat gaataaaaat ataaaatcac aataatttag
    27601 tatgtaattt taaattttaa aagatttaaa atgctctaag ttgttgtgtt tttttgtata
    27661 aatataaaat tataattctc t
//
LOCUS       DAMJYR010000040        27437 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.040, whole genome shotgun sequence.
ACCESSION   DAMJYR010000040 DAMJYR010000000
VERSION     DAMJYR010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 27437)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27437)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27437
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.040"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            262..2280
                     /locus_tag="PC471_001748"
     CDS             262..2280
                     /locus_tag="PC471_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HDF2788879.1"
                     /translation="MRRNTKLLTTGILSMAIVAPTMAFATESNAMENNADLNINLEKK
                     SIVLGSTSKVSVKFKEKPDADSITLKYKCYDMPLDTTLNYNQSTESYEGTINYNKDPE
                     YLNVWELQGITINSKNNPKTLNKQELEKMGLNLKDYNVTQECIIEDITSRKDVNKYLR
                     KTSAPITELTGSDRYETAVKISKEGWKNGSDKVVIINGDVSIDGIISTPLATTYNAPI
                     LLVEKNNVPNSVKSELKRLNPRDVIIIGDENAISKTTANQIKSTVNASQTRLKGSNRY
                     ETSLLIAKEIDKNHDVEKVYITNANGGEVDALTIAAKAGQDKQPIILTDKNSITDNTY
                     KWLKSEDLQNAYFIGGPQMISTNVINKVNDITKDNVTNNRVYGADRHETNANVIKKFY
                     TDDELEAVLVAKSDVLVDALAAGPLAANLKSPILITPKTYVSAYHKENLEAKSANKVY
                     KIGGGLTSKVMSSIASSLSKHNTTPTEPGNSGGKTVMIDPGHGGSDTGTTGKPLGGIR
                     EKDYTLNTSLATTEYLRSKGFNVIMTRDTDKTLSLGNRTALSNSLRPDLFTSIHYNAS
                     DTTGNGVEVFYKLKDKDGGTTKTVATNILNRILEKFNLKNRGAKTRTLSTDPTKDYLY
                     VLRNNDMPAVLVECAFLDNEKDMSLLNTSNKVKEMGTQIGKGIEDSLK"
     gene            2785..4797
                     /locus_tag="PC471_001749"
     CDS             2785..4797
                     /locus_tag="PC471_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HDF2788880.1"
                     /translation="MRRNTKLLTTGILSMAIVAPTMAFATESNAMENNADLNINLEKK
                     SIVLGSKSKVSVKFKEKPDADSITLKYKCYDMPLNTTLNYNQSTGAYEGTINYNQDPE
                     YLNVWELQGITINSKNNPKTLNRQDLEKLGLNLKDYNVTQECIIEDITSRKDVNKYLR
                     KTSSPITELTGSDRYETAVKISKEGWKNGSDKVVIINGDVSIDGIISTPLATTYNAPI
                     LLVEKNNVPNSVKSELKRLNPKDIIIIGDENAISKTTANQIKSTVNASQTRLNGSNRY
                     ETSLLIAKEIDKNHDVEKVYITNANGGEVDALTIAAKAGQDKQPIILTDKDSITDNTY
                     KWLKSEDLQNAYFIGGPQMISTNVINKVNGITKDSVTNNRVYGADRHETNANVIKKFY
                     TDDELEAVLVAKSDVLVDALAAGPLAANLKSPILITPKTYVSAYHKDNLEAKSANKVY
                     KIGGGLTSKVMSSIASSLSKHNTTPTEPGNSGGKTVMIDPGHGGSAPGNSSGGMIEKD
                     YNLNTSLATTEYLRSKGFNVIMTRDTDKTLSLGNRTALSNSLKPDLFTSIHYNGSTNK
                     QGHGVEVFYKLKDKNGGTTKTVATNILNRILEKFKLTNRGIKTRVLPSDSTKDYLYVL
                     RSNDMPAVLVECAFLDNENDMSLINSSAKVKEMGTQIGKGIEDSLK"
     gene            5039..6952
                     /locus_tag="PC471_001750"
     CDS             5039..6952
                     /locus_tag="PC471_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2788881.1"
                     /translation="MEYLFEKSSLKEYGNEALDKYRELLDKTGNNSRCILLLVPNNNT
                     KIRYERSLRLNYSEELKITTYISFVKKELVKYWPLIIEKCDEISTKVVSPTFISNSLS
                     EYLIVQKVKEKRIQEGYFEDITGTNKSIANSIMTNINKSAFNLIDFDFIGEKIYSSKK
                     NKDSIYRFSYTQMDEIISYYINSLLSNSILDNALSIYLYNQYLLNDDFYLKNLFTEVR
                     YIIVDSLESSSNAEVDFITEALDNTLQSYIYFDYSRDYSVFNNVDIDYINEKIISKIK
                     PKEENIKKKEIRIEDLYLLPANIELNQSSQLYNEMLDLISEKVISLIEEDVSPRDIAI
                     ISPINNSILEHQIRDSLIEKNIDVFSTKKDKKAVDYPYGNALVVATCIFYGYLDFIKD
                     DEYLSFLETLLEINRIKAFKIFNETRHLSIDNIDEHSQYREILQYIEEKKKSDIKIHE
                     FLTQFYIDKMLNLKEGKKNVGLCKKIIAESESFSESLSLLGMKKEKIFIEALKSTIND
                     YYSVVDIEELKSKDKIVITTPYSYISSNIDRSIQIWVDIGSNAWNMKIEKDISNLIVL
                     RKSFEEKKIYTNEMEEYYKKYYLYNTVYNLLLNAKKIYAFKSEYAINGYIQESILYSI
                     LLKISHKGDKNYD"
     gene            6945..9164
                     /locus_tag="PC471_001751"
     CDS             6945..9164
                     /locus_tag="PC471_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HDF2788882.1"
                     /translation="MININYREDQIPIINYDNGTMAVPAVPGAGKTFIITNLVAKLLL
                     EQKHKGGKILILTYMNSAVNNFKGRIRKILEENKIDDTNSYEVMTIHSLAVKIIKEKP
                     EVVMLSEDFNIADDLQKSIILNECISRFRFEGGERAFRWFLKEQKDERWKEITLEAWE
                     RGFFEFAGNAISELKYKGISPDNLEEILSRGHKGMLKIILPIYKLYDRKLKQNGLLDY
                     DDILILAEKTLSLDEGLRKKFQTRYKYIFEDECQDSNEIQGNIIKIISSENKNLVRVG
                     DINQSITGTFSSSDPKFFKEFINSADFCYRMDMSNRSSKDILDLANTLVKYVTKEFRQ
                     KECRHALEDMQIKTVPNGMGYKENPNPEHYNINVKWYESWKIEIEQTARYVKGIKNKY
                     PDKSIGILVPFNEQVTQVAKELRENNLTFEELGPNSLNKRKVINNIAYIIDFMLHCDD
                     VEKLIVVLNKVFIDTDNEEEIKYFLEELKNYSTEEIIYNFEFSKLDNDLIEFKEESDI
                     YLSFKRGIDIIKQILEYPIIRLDLLVLFIGKKLNLEKEDKAVLDYISFYIKYLVSENI
                     NMDLEDVYNLLFDVKNRVFNHIIDVVYEINGYEPEPGSITLCNYHKSKGMEWDCVFLL
                     GLVEYNFPDNINQKFQSDKWYLKEKYKNPMAIIKSEVEAILKGSISTDYAHKTKIDSI
                     NEKIRLLYVGITRAKEMLVLSGSAYRDESDIGNKRKEQKPCIYLSRLNQHIIEKRSN"
     gene            9273..10103
                     /locus_tag="PC471_001752"
     CDS             9273..10103
                     /locus_tag="PC471_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HDF2788883.1"
                     /translation="MNDKLKYFSYSQNSINTYKSCPLKFKYKYIDRINWKNDDVGSRE
                     YYETLKTGRDFHLICERYFSNIPLGIYFNEDDKNSKKFLKWMENIKKVVPIEKGKTYL
                     PEYEVRMTLNGDIIQAKYDLVVIGNNNIEIWDWKTESKKIDYKHVENRIQTIVYMFLA
                     KEVIPKVLKMDINVKDINMRYYQPEFDDLPITILYDEEKHEANRNKIQNYISMIKNTN
                     YEEHTYEDNLYNDIEKVYNKEERMCYRNKKHCKYCEFNKLCNGKEIDYSILEAEIYGT
                     "
     gene            10093..13560
                     /gene="addB"
                     /locus_tag="PC471_001753"
     CDS             10093..13560
                     /gene="addB"
                     /locus_tag="PC471_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddB"
                     /protein_id="HDF2788884.1"
                     /translation="MGLRFVLGRSGSGKSTYILDEIKKEAQKNETTSIILLVPEQYTF
                     EAENRVSKLFLGKEKDKYLRVRVLSFKTLSNIVFSQVGGLTDVNINSSGKAMMVYRAI
                     EDVSEELNVFSKSKSQSGFVSSITDMISEMKQYNISPEMLENISGELDNETLSLKLKD
                     ISKIYNSFEGKLHENYVDAQDMLTSLASKIELSSYLDGACVYIDEFTGFTPNQYNVIK
                     SILNKSKSVNISLTVDDINYIGYSKSDMFSRTKFTYSKLTQLCNEEGIKILPQVNLNT
                     GVIKRFEKVKELQHLERFYNAYPYKIYSNPTENIKIKEFNNLYSEVEEIAREIVHLVR
                     DKNVRYRDITIATRDLNRYDFLVHSIFNEYNIPNFIDKKREAKSNPIVILIISALEMK
                     NRRYGYETMFRYLKSGLIGIDNDDINLLENYVLANGIKGKKWFDEKWDYRITQSLSGQ
                     ESEFELELKEKINEIKNRVLEPIVILQEKLRGKNRVKEICRYIYEFLLDINMPETIES
                     LIVNFKDKGELDVANQYSQVWDIVVDILDQMVELMGDEIISLEKFIKLITLGFDEYEL
                     GLVPPSIDQVLVSSVDRMKNPDTKYLYLVGTTDGVFPLITKDSGILSDNDRESLGNKG
                     IEVDIDSKTRSFEEQFLVYKALTSTSKNLTITYPISDHEGKTLRPSIIISRLKKIFPN
                     IENKSYLVEENKSTDKDILKKITVKSPTFNELINVIKNYDSDGYNTEEINSIWLDIYR
                     YYLKDEIYSSITKKVIKGLSYTNQVHKIEEKKIRSLYKSNSLSISRLEKYAECPFAYF
                     IQYGLKAKKRKEYSFTPPDLGTFIHNILDRFSKELLQDNLTWRDIDEKYIELKIGIIV
                     DEIILKIPGYILNSSERYKYLAYRLKNMLTTAITIISQQIKQGSFEPIDYEVKFGNNG
                     KYPPIKMVLENGQEVSLIGQIDRVDEFEEGENKYIRIIDYKSGNKSISLTEIYYGLQL
                     QLLVYLDAILESAKDEDININPAAILYCRINNPIAKFNEDKDDEEIQEAILKELRMKG
                     LVVKDSHIVKEMDKSLIDGERKNSLVIPVGLTKDGNVGKSTSAISYEDFKLLRKYVRH
                     AIKDLCEEMLSGEIRIAPYKHKDGTSCDFCDYSAICQFDSTIKDNKYKNLNNKSNEEI
                     IKMMKGDVN"
     gene            13560..17387
                     /gene="addA"
                     /locus_tag="PC471_001754"
     CDS             13560..17387
                     /gene="addA"
                     /locus_tag="PC471_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HDF2788885.1"
                     /translation="MSSPKWTKEQLEVIESRECNLLVAAAAGSGKTAVLVERIIQMIT
                     SRENPIDIDKLLVVTFTNAAASEMRERIGDAIGKALDENPENKHLQNQLVLLNKSSIT
                     TIHSFCLDVIKSNFHRINLDPNFRIGDQTECAILKQEAIEEVFEDLYEERDEGFLNLV
                     ESYAERGGDKEVQDIILGIYSFAMASPEPKKWLIDSAERFNIDENFDFSQSIWARAIL
                     DTVKIEINGLCLNMERALKEVESIEELETFAEKLSVEYKKIADISQACNKSWDEAYKK
                     MASMSFENYVKGVKRISKDAPSYIKESKEKAKTIRDKTKKSLESIVSATFNKDNDSIR
                     EEIKYLYNIVKPISSVVLRFEEEYSNKKREKGIIDFNDIEHFALNILTDVDEKGNIVP
                     SDIAVGCRNKFYEIFIDEYQDSNLVQEVLLKAVANTETPNRFMVGDVKQSIYRFRQAK
                     PELFLQKYNNYNDKKGSSHRKIMLYKNFRSREEVVDAVNYIFENIMNENIGEIEYTEK
                     ERLNLGANFNVDTDEKSIIGGATEIHLIQKDNKLDDDIINDKDDRINNKENEIEEEEK
                     LDNIQLEARMVGNIIKDLMKVNEDGKIQKVYDKGIDGYRPVEFRDIVILLRATSAWAP
                     VFADELMNMDIPTYADVGVGYFDTIEIKTILSLLQIIDNPMQDIPLISVLKSPIFGFT
                     PEDLIDIRVQSKDKIFYEVLKSTAEYDGFTDSQNENESEFIPSEECINKSKDFLIKLK
                     EFKEKSMYMSTDEFIWYLYTRTGYYAYVGALPGGSQRQANLKVLFERAKQFEETSLKG
                     IFNFVNFIEKLKKSSSDMGSAKTLGENANVVRIMSIHKSKGLEFPVVICSAMGKNFNT
                     QDFKKSILYHHNLGYGPQFVDYERRISFPSIAKEALKSKINIENLSEEMRVLYVAFTR
                     AKEKLIITGSTRNIQDSIKRWSNGIESLDTISQYEILKGKNFLDWIMPCVLRHRDLSN
                     LLEEVGLDAVFNVEHNSKWYGKLWNKNDILVEKKSDEEKESIEEILEKIDVNNPDSDY
                     YGEIEEKLNYIYPYEFSTRKPATISVTEIKKIQNNYEEELINTIFEQKVILKKPLFIQ
                     NEEEREKISGTERGTIVHLVMEVLDLKNVSSVNDIKSQIRGFVSKGIITEKQASIVNP
                     YKIYKFFASNIGKRMLNAEIINREKSIYAQVNMKDIYIYEKLINNDDKKLYDNESVML
                     RGIVDAYFEEDNQIVLVDYKTDFVNEENINQIIEKYKKQLDLYADIIETLTGKSVKEK
                     CIYLFGVDEAVCY"
     gene            17433..18644
                     /locus_tag="PC471_001755"
     CDS             17433..18644
                     /locus_tag="PC471_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HDF2788886.1"
                     /translation="MRFIHTSDWHLGKSLEGHSRIEEQAKFCEEFIKIVESNEIDMVI
                     IAGDVYDTSNPPAQAEKLFYQTVSRLANNGQRCVLIISGNHDNPERLSAITPLAHEQG
                     ILIYGYPLSATIEAKYKGFEITYATQGCTKLNINGENIVVATLPYPSEKRLNEVFSSE
                     DEFEKQKNYSEKVGDIFRSLEENFRSDTINIAVSHIFVIGGESTESERPIQLGGSFLV
                     ERKDLPEKAQYTALGHLHKQQKASERLNAYYSGSPLQYSKDERAYTKGAYIVDIKAGE
                     KPIIEDVYFNNYKPIEVFKCNGIEEALDICEQNQDREIWSYFEINTDEIISQNEIKKM
                     KELLKDIIEIKPIITSCYEQESVDIKEKSMAELFREFYSFSKGVEPKGELMDLFLDII
                     SEEGESADETN"
     gene            18631..22158
                     /locus_tag="PC471_001756"
     CDS             18631..22158
                     /locus_tag="PC471_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDF2788887.1"
                     /translation="MRPIRLELTGLNSYIDKQVIDFEKLIERGLFGIFGTTGSGKSTI
                     LDAITIAMYGNISRNTKEYINSVCDKAIISYEFEIGSKNTRRRYIVDRTIARSKTGTK
                     TSNARLIEVLNDNTQNVLADKVVEVNEKVAQVVGLTANDFTRSVVLPQDKFNEFLRLS
                     RADRRDMLERIFNLEKYGRSLGEKVKKRKNIQLQNLKDLKSKLSQYDGITEEVYNNIN
                     QELIELKNLEKDKDNALDLAQKSYEESKTVYEEQLKLEKNELRKNELDLKNSEIKEKI
                     ALVENDSNAKKVDPYISSVQNLEKKIDEDSFAVSNLEKKLAILNQELEVTKNRYEKIN
                     KIKNEEVPKLSEEKIRLQQAIKLEEELVLLDRELKELKESGINLNKTKVELEKVKQVS
                     ESRKDAVTKSIKEVEGKIDKVNISAELKQKIFLAYEYEKDYNNVLEEKNQKLNKLEEI
                     LKETENINLKVRYIDKDKNDVNRNLENLSLHLDVLLKKCPGKSADLLLKSEYVTELRN
                     KANNTKENEIKKSSIQDELKIILESKFNTEREVNLLNEKLENNRKNRDDLEKELEELK
                     YLNLASELRRELKENMPCPVCGSKHHENHDITKYDENISFVKEKLEKLEKEKISIRNN
                     IEELNAKVSGYLSIEKMKTKELEDVKGKLGGIPSSQLLKKLDEEQRKLALLKSNIQEW
                     EKEKESTENKVTLAKEEKNKIEKEELKIRESLNNYKKLTRDLNIEIEGLENKCKKLKQ
                     EYLGLKTITKVSNLLSKVKEIRENEKELELLSTGHSNLLKNRDSLDIKIRENESKLHE
                     IEIELIKARELYVEKKLSRDNKYKEVISITKGDLAKNLLHNVEENICKILEQEESSKK
                     KLEEQRLEYEKNIAEKHNIDGRLKTAKEQYKDQKSTLNKLLAENKFESIYAVKRALLD
                     TDAVKKLIEEILEHEEEQKLLSFKIKSSKEKLNGRSIKKEYFEQLKDEIYNLKVEIGN
                     ISKDIGANQNQLITLKDSLDKINDFNKQLKVVEHNVDLLEELDKCVQGNKFVEYVSTN
                     QLKYIALEASKRLDGITKGRYALEIDSTLNFVMRDNFNGGERRSIDTLSGGETFLTSL
                     SLALALSSQIQLKGSAPLEFFFLDEGFGSLDSELLEIVIESLERLHSNNLSVGIISHV
                     EELKNRVPVKLLVSSSEAGIGSKVKIEYS"
     gene            22289..23542
                     /locus_tag="PC471_001757"
     CDS             22289..23542
                     /locus_tag="PC471_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HDF2788888.1"
                     /translation="MLESPNNLIQIIFLIVLLIGSAFFSASETALMSLSKIRIRYMQD
                     EGVKGAKLVSSLIENPNKLLSSILVGNNVVNIAATSISTSLFIGLMGEKGVALATAVM
                     TVLVLIFGEITPKTIAANNSEKVSLLVSKPIKAIIFILRPIVWIFNIITNIIFKLFGI
                     TNKGAKSFITEEELKTMVNVSHEEGVLEMEEREIINNVFEFGDMQAKNAMVQRIDMVA
                     IDMEDSYDEIIQVFKTEKLSRMPVYEETIDDIVGILNIKDIIFLSDEEIESFDIKNYM
                     REPFFTYEFKKITQLLEEMKLEKSQMAIVVDEYGGTSGLLTIEDLVEVIVGDIEDEYD
                     EEEDEIQVIKEDEYIVDGSTKIGDVNELIGVNLESEEFDSIGGFIIGHLSRLPEENEV
                     IEVDNIRFCIESIEKNRIKKIRIYT"
     gene            23691..24743
                     /gene="ytvI"
                     /locus_tag="PC471_001758"
     CDS             23691..24743
                     /gene="ytvI"
                     /locus_tag="PC471_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation integral membrane protein YtvI"
                     /protein_id="HDF2788889.1"
                     /translation="MSILENNFLYKLKNNTIFFCIYTLLFIFIYKAFPYIAPFFLGAI
                     IALMINPISQKLENKFRINKGISTLVLSFLAVAIVSTVTTIIVINSMKELMGFLNNIS
                     ANPEDISNTIMYFLNKINDFMKSFQEIANFDVEQLVNKFSGEVMQITKNLLTSILGLA
                     TSIPYIIIFIITLFIATYFIAKDLDKIENSFYNMFTVDVRKKVKNVKKEAGLSLVGYI
                     RAYTILMAITFFAIWGSFALFGLKYGLIVGFVGALMDLIPFLGIITIYLPVIVYYFLI
                     KNYFIAISMTVIFFVLSLIREILEPKLVSVNVGLNPLATLAAIFIGIQVKGIIGVIFC
                     LGLVCMHDILKKVDIF"
     gene            24833..26059
                     /gene="pepT"
                     /locus_tag="PC471_001759"
     CDS             24833..26059
                     /gene="pepT"
                     /locus_tag="PC471_001759"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HDF2788890.1"
                     /translation="MKQKVVERFLKYVSFDTTSNSQCENCPSSEGQRVLAKYIVEELK
                     TMGVDDVSLDENSYIMATLKGNTDGVDTIGFISHLDTIEDVSGKDIKPRIIENYDGKD
                     IVLNEALNVITYVKDSPELEEFKGDDLIVTDGTTLLGSDDKAGIAEIVTAIEYLINHP
                     EIKHGDIKIGFTPDEEIGRGADLFDVEKFGAKYAYTLDGGIVGELECENFNAANATIT
                     IHGRNVHPGSAKNKMVNAIHIAAEISEMFPADERPETTEGYEGFWHLNSIGGNVENVS
                     MAYIIRDHCKEKFENRKSIMIENIEKINKKYDNRVELDLKDSYYNMKEKIEPVMFIVD
                     IAKEAMEELGIKPRLVPVRGGTDGARLSFNGLPCPNIFTGGLNFHGKNECIPVSSMEK
                     ATKLIVRIAEKYAERV"
     gene            26266..27318
                     /locus_tag="PC471_001760"
     CDS             26266..27318
                     /locus_tag="PC471_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp18"
                     /protein_id="HDF2788891.1"
                     /translation="MKIKSKKIIAFALSLGIMLPSCVYADTISKNQNAQVDMNNYLQG
                     RKSPKEEKLIGEDRFDTAIKISQSGWNNGSERVFLVNSNSLPDALASTPLASKLDAPI
                     LLTNKNSIPYNVTEEIKRLNPAEVILIGSEGAISSSVKESLEDMEVLVSRIGGADREE
                     TSLLLTRQLDDTGDLGVSKVAVVNGYNGLADATSISSPAASDNTAIIYTGKDSIRSEA
                     KNFITQNSTKTYIVGGEYSISKKLEGQLVNSERLAGTDRKDTNAKVLEKFYGKSSKVN
                     NMYFAKDGSGREADLVDGLAAGVLASKTKSPVVLASGSLSSAQKSFIKKVKADKFVQV
                     GGGKNSKPYAEALALQ"
BASE COUNT        11666 a         2682 c         4851 g         8238 t
ORIGIN      
        1 ttgtattaaa tttaataaac ctacaattgt tatatgtttt tatttgtctt ttatagattt
       61 ttttggcaat aaatgatata gtgaagataa ttaaatatat tcaatatatt taattttttt
      121 attttttgtt tttataattt ttttagaagt ttatttttta tgtacttatt tataattttg
      181 ggtaaaaaca ctttgatata atttcaaaaa ttaaaaggtt aaaaaacaaa gtagaaattt
      241 atgttaaata aggagattaa tatgagaaga aatacaaaat tattaacaac agggattctt
      301 tcaatggcaa tcgtcgcacc tacaatggca tttgctactg aatctaatgc tatggaaaat
      361 aacgctgatt taaatataaa cttagagaaa aaaagtatcg ttttaggtag tacctcaaaa
      421 gtcagtgtta agtttaaaga aaaaccagat gcagatagca ttacattaaa gtataaatgc
      481 tatgatatgc cattggatac aactttaaac tataatcaat caactgagtc atatgaagga
      541 actatcaatt ataataaaga cccagaatat ctaaatgttt gggaactaca agggataaca
      601 ataaacagca aaaataatcc taaaacttta aacaaacaag aactagaaaa gatgggatta
      661 aatttaaaag actataatgt aacgcaggaa tgtataattg aagatataac ttctagaaaa
      721 gatgtaaata aatatttaag aaaaacttct gcacctatta cagaacttac aggaagtgat
      781 agatacgaaa cagcagttaa aataagtaaa gaaggctgga aaaatggttc agataaggta
      841 gttataataa atggggatgt aagtatagat ggcattatat caactccact agcaactaca
      901 tataatgcgc caatactttt ggttgaaaaa aacaatgtac ctaatagtgt aaaatcagaa
      961 ttaaagcgtt taaatcctag agatgtaatt ataattggag atgagaatgc tatttctaaa
     1021 actactgcta atcaaattaa atcgactgta aatgctagtc aaacacgtct aaagggctct
     1081 aatagatatg agacatcttt attgatagca aaggaaatag ataaaaatca tgatgtggaa
     1141 aaagtataca taacaaatgc taatggcgga gaagtggatg cacttactat agcagcaaaa
     1201 gcaggtcaag acaagcaacc aattatatta actgataaaa atagtattac agacaacaca
     1261 tataaatggt taaagagtga ggatttacaa aatgcttatt ttataggtgg ccctcaaatg
     1321 atatcaacaa atgttataaa taaggtaaat gatataacta aagataatgt tactaataat
     1381 agagtatatg gagcagatag acacgaaaca aatgcaaacg taataaaaaa attctataca
     1441 gatgatgagt tagaggctgt tttagtagct aaatcagatg tacttgttga tgctttagca
     1501 gcaggtccat tggctgcgaa cttaaaatct ccaatactta taacaccaaa gacatatgta
     1561 tctgcatacc ataaagaaaa cttagaagct aaatcggcaa ataaggtata taaaataggt
     1621 ggaggattga cttctaaggt aatgagctct atagcatcat cattatctaa acacaataca
     1681 actccaacag aaccaggaaa tagtgggggc aagacagtta tgattgaccc aggacatggt
     1741 ggttcggaca ctggaacaac aggtaaacca ttagggggta ttagagaaaa agactataca
     1801 ttaaatactt cacttgcgac aactgaatat ttacgttcaa agggattcaa tgtaataatg
     1861 acaagagaca cagataagac tttatctctt ggaaatagaa ctgctttatc taactctttg
     1921 agaccagatt tatttacgag tatacactat aatgcatctg atacaactgg gaatggtgta
     1981 gaagtatttt ataagcttaa agataaagat ggagggacta ctaaaactgt agctacaaat
     2041 atattaaata gaatcttaga gaaatttaat ctaaaaaata gaggtgcaaa aacaagaaca
     2101 ctatcaactg accctacaaa agattattta tatgttttaa gaaataatga tatgccagct
     2161 gtacttgtag aatgtgcatt tttggataat gagaaagata tgagtttact taatacatca
     2221 aataaagtaa aagaaatggg tacacaaata ggtaaaggca tagaagattc attaaaatag
     2281 tctacaatga gagcatagaa aaatttaaaa taaattaatc tgcaatttta aatttaaact
     2341 aaagctataa gataagaatg taattataat agtaataaag actgtatata cattgtgcat
     2401 ataagtgcga aatgaggtat atacagtctt tttatgatta attgtaaaag gatttatatt
     2461 atatgaagaa tttacatatc ttaaggctac agttaattgt caagagttgg agtattcatt
     2521 tgtaatacac aataaaagct taattactat attttattgt gtgtttgtgt gttttttgtg
     2581 gatttttatg tcaataaatg atatagtata gataattaaa tgtattaaat atatttaatt
     2641 tttttatttt ttgagtttta taagtttatt tttatatatt acttatatat tatttatatt
     2701 ttgggttaga tacactttga catattttca aaagttaaaa gattaaaaat aaagtagaaa
     2761 tttatgttaa gtaaggagat taatatgaga agaaatacaa aattattaac aacagggatt
     2821 ctttcaatgg caatcgtcgc acctacaatg gcatttgcta ctgaatctaa tgctatggaa
     2881 aataacgctg atttaaatat aaacttagag aaaaaaagta tcgttttagg tagcaaatca
     2941 aaagttagtg tcaaatttaa agaaaaacca gatgcagata gcattacatt aaagtataaa
     3001 tgctatgaca tgccattgaa tacaactcta aattacaatc aatcaactgg ggcatatgaa
     3061 ggaactatca attataacca agacccagaa tatctaaatg tttgggaact acaagggata
     3121 acaataaaca gcaaaaataa tcctaaaact ttaaacagac aagacctaga aaagctggga
     3181 ttaaatttaa aagactataa tgtaacacag gaatgtataa ttgaagatat aacttctaga
     3241 aaagatgtaa ataaatattt gagaaaaact tcttcaccta ttacagaact tacaggaagt
     3301 gatagatatg aaacagcagt taaaataagt aaagagggct ggaaaaatgg ttcagataag
     3361 gtagttataa taaatgggga tgtaagtata gatggcatta tatcaactcc actggcaacc
     3421 acatataatg caccaatact tttggttgaa aaaaacaatg tacctaatag tgtaaaatca
     3481 gaattaaagc gcctaaaccc taaagatata attataattg gagatgagaa tgctatttct
     3541 aaaactactg ctaatcaaat taaatcaact gtaaatgcta gtcaaacacg tttaaatggt
     3601 tctaatagat atgagacatc tttattgata gcaaaggaaa tagataaaaa tcatgatgtg
     3661 gaaaaagtat acataacaaa tgctaatggc ggagaagtgg atgcacttac tatagcagca
     3721 aaagcaggtc aagacaagca accaattata ttaactgata aagatagtat tacagacaat
     3781 acatataaat ggttaaagag tgaggattta caaaatgctt attttatagg tggtcctcaa
     3841 atgatatcaa caaatgttat aaataaggta aatggaataa ctaaagatag tgttactaat
     3901 aatagagtat acggagcaga tagacacgaa acaaatgcaa acgtaataaa aaaattctat
     3961 acagatgatg agttagaggc tgttttagta gctaaatcag atgtacttgt tgatgcttta
     4021 gcagcaggtc cattggctgc gaacctaaag tctccaatac ttataacacc aaagacgtat
     4081 gtatctgcat accataaaga taatttagaa gctaaatcag ctaataaggt atacaaaata
     4141 ggaggaggat tgacttctaa ggtaatgagc tctatagcat catcattatc taaacacaat
     4201 acgactccaa cagaaccagg aaatagtggg ggcaagacag ttatgattga cccagggcat
     4261 ggtggttcag cacctggaaa ttcatctgga ggaatgattg aaaaagatta caatttaaat
     4321 acttcacttg caacaactga atatttacgt tcaaagggat tcaatgtaat aatgacaaga
     4381 gacacagata agactttatc tcttggaaat agaactgctc tatctaattc attgaaacca
     4441 gatttattta caagtataca ttataatggc tcaactaata aacaaggtca tggtgtagaa
     4501 gtattttata agcttaaaga taaaaatgga gggactacta aaactgtagc taccaatata
     4561 ttaaatagaa ttttagagaa atttaaactt acaaatagag gtataaaaac aagagtactt
     4621 cctagtgatt ctacaaaaga ttatttatac gttttaagaa gtaatgatat gccagctgta
     4681 cttgtagaat gtgcattttt ggataatgaa aatgatatga gtttaataaa ctcatctgca
     4741 aaagtaaaag aaatgggtac acaaataggt aaaggaatag aagattcatt aaaataaatg
     4801 gaaataaaaa tatttaaaaa cttaatgtaa atttatagtt gtaaatttat actaaagtca
     4861 aaaaatgaaa tataagtgta tcaatagtaa gactgtatgt acatttgcac gtgtgaatgc
     4921 aagtatatat acagtctttt tttgtagtta aatgcaataa tttgaatgtg ttttgtaaaa
     4981 tacacaatat gatataatta gataatatac acttaattta attgaggaag gtgattatat
     5041 ggaatattta tttgaaaaaa gtagtttaaa agaatatgga aatgaagctt tagataaata
     5101 tagagaatta ttagataaaa ctggtaataa ttcaaggtgt attcttttgt tagtaccaaa
     5161 caataacaca aagatacgat atgaaaggtc tctaagatta aattatagtg aagaactgaa
     5221 aataacaaca tatataagtt ttgtgaagaa agaattagtt aaatactggc cactaataat
     5281 agaaaaatgt gatgaaataa gtacaaaggt tgtatctcca acatttatat caaatagttt
     5341 aagtgaatat ttaatagtgc aaaaagttaa ggaaaaaagg atacaagaag gatattttga
     5401 agatattaca ggaaccaata aatccatagc aaatagtata atgactaaca taaataagtc
     5461 tgcatttaat ttaattgatt ttgattttat tggagaaaaa atatattcat caaagaaaaa
     5521 taaagatagt atatatagat tttcatacac tcaaatggat gaaattatat catattatat
     5581 aaattcctta ctatcaaatt caattttaga taatgcatta agcatatatt tatacaatca
     5641 atacctactt aatgatgatt tttatttaaa aaacttattt acagaagtga gatatataat
     5701 tgtggatagc cttgaaagct caagtaatgc agaagttgat tttataacag aagcattgga
     5761 taatacatta caaagttata tatattttga ttatagtagg gattattctg tatttaataa
     5821 tgtggatata gactatataa atgagaaaat aatatcaaaa attaagccta aagaagaaaa
     5881 tattaaaaag aaggagatta gaatagaaga tttatactta ttacctgcaa atatagaatt
     5941 aaatcaatca agtcaattat ataatgaaat gttagattta ataagtgaaa aagttatatc
     6001 tcttatagaa gaagatgtat ctccaagaga tatagcaatt atatctccta taaacaatag
     6061 tatattggag catcaaatta gagattcttt gatagaaaaa aatatagatg tttttagtac
     6121 taagaaggat aaaaaagctg tggattatcc atatgggaat gcattggtag tggctacatg
     6181 tatattttat ggatatttag attttataaa agatgatgaa tacttaagct ttttagaaac
     6241 tcttttagaa ataaatagga taaaagcatt taagattttt aatgaaacaa gacatttaag
     6301 tattgataat atagatgaac atagtcagta tcgtgagatt ttacagtata ttgaagaaaa
     6361 aaagaagagt gatataaaaa tacatgaatt tttaactcaa ttttacatag ataaaatgtt
     6421 aaatttaaaa gaaggtaaaa agaatgttgg gttatgtaaa aagataatag ctgaaagtga
     6481 gtcttttagt gaaagtttaa gtctacttgg gatgaaaaaa gaaaagatat ttatagaagc
     6541 tttaaaaagt acaataaacg attattactc tgttgtcgat atagaagagt tgaaatccaa
     6601 agacaaaata gtaataacta ctccttattc atatatatca tcaaatatag atagaagtat
     6661 acaaatatgg gtagatatag gaagtaatgc ttggaatatg aaaatagaaa aagatataag
     6721 taatttaatt gtacttagaa aatcttttga agaaaaaaag atttatacaa atgaaatgga
     6781 agaatattat aaaaaatact atctatacaa tacagtatat aatttgttat taaatgcaaa
     6841 gaaaatctat gcatttaaaa gtgaatatgc gattaatggt tatatccaag agagtatatt
     6901 atatagtata cttttaaaaa tctcacataa aggagataaa aattatgatt aatataaatt
     6961 atagagaaga ccagatacca ataataaatt acgataatgg tactatggca gtaccagcag
     7021 taccaggtgc tggaaaaaca tttataataa caaatttagt agctaaacta ttacttgagc
     7081 agaaacataa gggtggtaaa atattaattc ttacatatat gaatagtgct gtaaataatt
     7141 ttaaaggtag aataagaaaa attcttgaag agaataaaat agatgatact aattcgtatg
     7201 aagtaatgac tatacacagt ttggctgtaa aaataataaa ggaaaagcca gaagttgtaa
     7261 tgttaagtga ggattttaac atagctgacg atttgcaaaa aagtattata ttaaatgaat
     7321 gtataagtag atttagattt gaaggtggag aaagagcttt tagatggttt ttaaaggagc
     7381 aaaaagatga acgctggaaa gaaataactc ttgaagcctg ggaaagaggt ttttttgaat
     7441 ttgcaggaaa tgcaataagt gaacttaagt ataagggaat ttctccagat aaccttgaag
     7501 aaatattgtc aagaggacac aaaggaatgc ttaaaattat tttaccaata tataaattgt
     7561 atgatagaaa attaaagcaa aatggattgc tagattatga tgatatatta attttagcag
     7621 aaaaaacttt aagcttagac gaagggctta gaaagaagtt tcaaactaga tacaaatata
     7681 tatttgaaga tgagtgtcag gactcaaatg aaattcaagg taatataata aaaattatat
     7741 ctagcgaaaa taaaaaccta gttagagttg gagatataaa tcagagtata acaggaacat
     7801 tttcatcatc agaccctaaa ttttttaaag aatttataaa tagtgcagat ttctgttata
     7861 ggatggatat gtcaaatcga agttcaaaag atatacttga tttagcaaac actcttgtca
     7921 agtatgttac aaaggaattt agacagaaag aatgtagaca tgctcttgaa gatatgcaaa
     7981 taaaaactgt gccaaatgga atgggatata aagaaaatcc taatccagaa cattataata
     8041 taaatgtaaa gtggtatgag agttggaaaa ttgaaataga gcaaactgct agatatgtta
     8101 aaggtataaa aaataaatat ccagataaaa gtataggtat attggttcca tttaatgaac
     8161 aagtgacaca agttgctaaa gaacttagag agaataatct aacttttgaa gaattaggac
     8221 caaattcttt gaataaaagg aaggttataa acaacatagc atatattatt gattttatgt
     8281 tacattgtga tgatgtagaa aagttaatag ttgtacttaa taaagttttt atagatactg
     8341 ataatgaaga agaaataaag tattttttag aagaattaaa aaactattct actgaagaaa
     8401 ttatttataa ttttgaattt agtaagttag ataatgattt aatagaattt aaagaagaaa
     8461 gtgacatata tttaagtttt aaaagaggaa tagatattat aaaacagata ttagaatatc
     8521 caataataag gttagacctc ttagttttgt ttattgggaa aaaacttaat ttagaaaaag
     8581 aagataaagc tgtactggat tatatatctt tttatataaa gtatctagta tcagaaaata
     8641 taaatatgga tttagaagat gtatataatt tattgtttga tgtaaagaac agggtgttta
     8701 atcatataat agatgtggta tatgagataa atggatatga accagagccc ggtagtataa
     8761 cactatgtaa ctatcataaa tcaaagggga tggaatggga ttgtgtgttt ttactaggtc
     8821 ttgttgaata taatttccct gacaatatta atcaaaagtt tcagagtgat aaatggtatc
     8881 taaaagaaaa atataaaaac cctatggcaa ttattaaatc agaagttgaa gctatattaa
     8941 aaggaagtat ttcaacagat tatgctcata aaacgaagat tgattctata aatgaaaaga
     9001 ttagactttt atatgtaggg attactagag caaaggaaat gttagtactt tcaggaagtg
     9061 cttatagaga tgaaagtgat atagggaata agagaaaaga gcaaaaacca tgtatatatt
     9121 taagcagact taatcaacat attattgaaa aaaggtcaaa ttaatatata ataagttatt
     9181 aaaagattgc ttgaaatata tctaaaaggg gataaaacaa tatgtacagt aagtaactac
     9241 taaaaaataa agttaagaga gaagtgatat gagtgaacga taaattaaaa tatttttctt
     9301 atagtcaaaa ttcaattaac acatataaat catgtccatt gaaatttaaa tataaatata
     9361 ttgatagaat aaattggaaa aatgatgatg ttggtagtag agagtattat gaaactttaa
     9421 aaacaggaag agattttcat ttgatttgtg aaagatattt tagcaatata cctcttggaa
     9481 tctattttaa tgaggatgat aaaaatagta aaaagttttt aaagtggatg gaaaatataa
     9541 aaaaagttgt tcctattgaa aaaggaaaaa cgtatttgcc agaatatgaa gtaaggatga
     9601 cattaaatgg ggatataata caggcaaagt atgatttagt tgtaattgga aataacaata
     9661 tagaaatatg ggattggaaa accgagtcta aaaagattga ctataaacac gtagaaaata
     9721 gaatacaaac tattgtttat atgtttttag caaaagaagt aatacctaaa gttttaaaaa
     9781 tggacataaa tgttaaagat ataaatatga gatattatca acctgaattt gatgacttac
     9841 caattaccat tttatatgat gaagaaaaac atgaagcaaa tagaaataaa attcaaaatt
     9901 atataagtat gataaaaaac actaattatg aagaacatac atacgaagac aatctataca
     9961 atgatataga aaaggtatat aataaagaag aacgtatgtg ctatagaaat aaaaagcatt
    10021 gcaagtattg tgaatttaat aagttatgta atggcaaaga aatagattat agtattttgg
    10081 aggcggaaat ttatgggact taggtttgta ttaggtagaa gtggaagtgg aaaatccacc
    10141 tatatactag atgaaattaa aaaagaagct caaaaaaatg aaacgacatc aattatactt
    10201 ttagttccag aacagtacac atttgaggct gaaaatagag taagtaaact atttttagga
    10261 aaagaaaaag ataagtatct aagagttagg gtacttagct ttaagacact aagtaatata
    10321 gttttttctc aagtaggagg attgacagat gtaaatataa attctagtgg aaaagcaatg
    10381 atggtatata gagctattga agatgtaagt gaagaattaa atgtgttttc aaaatcaaaa
    10441 tcgcagtctg gttttgtaag ttcaattaca gatatgatat cagaaatgaa acaatacaat
    10501 ataagccctg aaatgttaga aaatatatct ggagaattgg ataatgaaac tttaagttta
    10561 aaattaaaag atataagtaa aatatataat tcttttgaag ggaaattgca tgaaaattat
    10621 gtagatgctc aagatatgtt aacttcattg gcaagtaaaa ttgaattaag cagttattta
    10681 gatggagctt gtgtttatat agatgaattt acaggtttta caccaaatca gtacaatgtc
    10741 ataaagagta tattaaataa gtctaaaagt gttaatatct ctttgactgt agatgatatc
    10801 aactatattg ggtacagtaa atctgatatg ttttctagaa cgaaatttac ttattcaaaa
    10861 ttgactcaat tgtgcaacga agaaggcata aaaatattgc cacaagtaaa tttaaacaca
    10921 ggtgttataa aaagatttga aaaagttaag gaattacagc acttagaaag attttataat
    10981 gcttatccat ataaaatata ttcaaatcca actgaaaata taaagataaa agagtttaac
    11041 aatttatatt cagaggtgga agagatagcc agagaaattg tacatttagt tagagataag
    11101 aatgtcagat atagagatat aaccattgct acaagagatt taaatagata tgatttttta
    11161 gtacactcta tatttaatga atataatata ccaaacttta tagataagaa gagagaggca
    11221 aaaagtaatc caatagttat tttaattatt tcagctttag aaatgaagaa tagacgatat
    11281 gggtatgaaa ctatgtttag gtatttaaaa agtggactta taggaattga taatgatgat
    11341 ataaatttac tagaaaatta tgtgttggct aatgggatta aaggtaaaaa atggtttgat
    11401 gaaaaatggg attatagaat aacccaaagt ctttcaggtc aagaaagtga atttgaactg
    11461 gaattaaaag agaaaataaa tgaaataaaa aatagagtat tagagcctat agtaatttta
    11521 caagaaaaat taagaggaaa aaatagagta aaagaaatct gtagatatat atatgaattt
    11581 ttattagata taaatatgcc agaaactata gaaagtttaa tagtaaattt taaggataaa
    11641 ggggaacttg atgttgcaaa tcaatactca caagtttggg acatagtagt ggacatatta
    11701 gaccaaatgg tggaacttat gggtgatgaa ataatttctt tagaaaaatt tataaaatta
    11761 ataaccttag gttttgatga atatgaatta ggactggttc ctcctagtat agaccaggta
    11821 cttgtaagta gtgtggatag aatgaaaaat ccagacacga aatatttata cctagttgga
    11881 actactgatg gtgtgtttcc gctgattaca aaggatagtg gaatattgag tgataatgat
    11941 agggaaagtc ttggaaacaa aggaatcgaa gttgatattg atagtaagac taggagtttt
    12001 gaagaacagt ttcttgtata taaagctctt acttcgacat ctaagaattt aaccataaca
    12061 tatccaatat cagaccatga aggtaaaacg cttagaccat caataattat atcaagactt
    12121 aagaagatat ttccaaatat agaaaataaa agttatttag tggaagaaaa taaaagcact
    12181 gataaagata tacttaaaaa aataacggtt aaatccccta catttaatga acttataaac
    12241 gtaataaaaa attatgacag tgatggttac aatacagaag agattaatag tatttggtta
    12301 gatatatata ggtactattt aaaagatgaa atctatagtt ctataacaaa aaaagttata
    12361 aaaggtctta gttatactaa tcaagttcat aagattgaag agaaaaaaat aaggtcattg
    12421 tataaaagta attctcttag catatctagg cttgaaaaat atgctgaatg tccatttgct
    12481 tactttatac aatatggtct aaaagcaaaa aagcgtaaag agtattcatt tacaccacca
    12541 gatttaggaa cttttattca taatatttta gataggtttt caaaagaact tttacaagat
    12601 aatttaactt ggagagatat tgatgaaaaa tatatagaat taaagatagg tataatagtt
    12661 gatgaaataa ttttaaagat accaggatat atattaaata gttctgagag gtataaatac
    12721 cttgcatata gattaaagaa tatgttgaca actgctataa ctataattag tcagcaaata
    12781 aaacaaggtt catttgaacc aatagattat gaagttaaat ttggcaacaa tggaaaatat
    12841 ccacctataa aaatggtgtt agaaaatgga caagaagtaa gtttaattgg acaaatagat
    12901 agagttgatg aatttgaaga aggagaaaat aaatatataa gaataataga ttataagtca
    12961 ggcaataagt ccataagtct aacagaaata tattatggtc tacaattaca attacttgtg
    13021 tatttagatg caatccttga aagtgcaaag gatgaagata taaatataaa tccagctgca
    13081 atactatatt gtagaataaa taatccaata gcaaaattta atgaagataa agatgatgaa
    13141 gaaatacaag aagcaatatt aaaagaactt agaatgaagg ggcttgtagt aaaagactct
    13201 cacatagtta aagagatgga taaatcatta atagatggag aaaggaaaaa ctctttagta
    13261 ataccagtag gtcttactaa ggatggaaat gtaggtaaaa gtacttcagc aataagctat
    13321 gaggatttca aactgcttag aaaatatgta aggcatgcta taaaggattt atgtgaagaa
    13381 atgttaagtg gagaaatacg tatagctcca tataaacata aagatggaac atcatgtgac
    13441 ttttgtgatt actctgctat atgtcagttt gattcaacta taaaagataa caagtataaa
    13501 aatttaaata ataagagtaa tgaagagata ataaaaatga tgaaaggaga tgttaattag
    13561 tgagttctcc taaatggaca aaagagcaat tagaagttat agaaagtaga gaatgcaatt
    13621 tattggttgc tgctgctgct ggttcaggaa aaacagccgt tctagttgag cgtataattc
    13681 aaatgataac aagtagagaa aatccaattg atatagataa gttattagtt gttacattta
    13741 ctaatgctgc tgcgtcagaa atgagagaga ggatagggga tgctatagga aaagccttag
    13801 atgaaaaccc agaaaataaa catttacaaa accaacttgt tttattaaat aaatctagta
    13861 ttacaacaat acactcattc tgtttggatg ttataaaatc aaactttcat agaataaact
    13921 tagaccctaa tttcagaata ggagaccaaa ctgaatgtgc tattttgaaa caagaagcaa
    13981 tagaggaagt atttgaagat ttatatgaag aaagagatga ggggttttta aatttagttg
    14041 aaagttatgc agaaagagga ggagataaag aagtacaaga tattatcttg ggaatatatt
    14101 catttgcaat ggcttctcct gaacctaaaa aatggttgat tgactcagca gaacgattta
    14161 atattgatga aaattttgat ttttcacagt ctatttgggc tagagctata ttagacactg
    14221 taaaaataga gattaatggt ctttgtctaa atatggaaag ggcattaaaa gaagtggaat
    14281 caatagaaga attagaaact tttgcagaaa agttatccgt tgaatataaa aaaattgctg
    14341 acatttcaca agcatgtaat aaatcttggg atgaggcata taaaaagatg gcaagtatgt
    14401 cctttgaaaa ttatgtaaaa ggagtaaaaa gaatttcaaa agatgctcct tcgtatataa
    14461 aagaatcaaa agaaaaggca aaaactataa gagataagac taagaagtct ttagaaagta
    14521 tagtaagtgc tacattcaat aaagataatg attctattag agaagaaata aaatatcttt
    14581 ataacatagt taaaccaata tctagtgttg tactgagatt tgaagaggaa tatagcaata
    14641 agaaaagaga aaaaggaata atagatttta acgatataga gcattttgca ttaaatatac
    14701 ttacagatgt agatgaaaaa ggtaatattg taccttctga tatagcagtt ggatgtagaa
    14761 acaagtttta tgaaatattt atagatgaat atcaggatag taatctagtg caggaagtac
    14821 tgcttaaagc tgttgcaaat acagaaactc caaatagatt tatggttggt gatgtaaaac
    14881 agagtatata tagatttaga caagcaaagc cagaattatt tttgcaaaaa tataataact
    14941 ataatgataa aaagggaagc tcccatagaa aaataatgct ttacaaaaac tttagaagta
    15001 gagaagaagt tgtagatgct gtaaattata tatttgaaaa tataatgaat gagaatatag
    15061 gagaaattga atatacagaa aaagaaagac taaatttagg tgcaaatttt aatgtggata
    15121 cagacgagaa atctattatt ggtggagcaa cggagataca cctaatacaa aaagataata
    15181 aacttgatga tgatattata aatgataaag acgatagaat taataacaaa gaaaatgaaa
    15241 tagaggaaga agaaaaatta gataatatac aacttgaggc tagaatggtt ggaaacatta
    15301 tcaaagattt aatgaaagtc aatgaagatg gaaaaattca aaaggtctat gataagggaa
    15361 tagatggata tagacctgtg gaatttagag atatagttat tcttttaaga gctacatctg
    15421 catgggctcc agtgtttgca gatgaattga tgaatatgga tataccaacg tatgctgatg
    15481 ttggagtggg gtattttgat acgattgaaa taaaaacaat attgtcatta cttcaaataa
    15541 tagataatcc aatgcaagat atacctctta tatctgtatt aaaatcacct atatttggat
    15601 ttacaccaga ggatttgatt gatattagag ttcagagtaa agataaaatt ttttatgagg
    15661 ttttaaaaag tacagcagag tatgatggat ttacagattc tcaaaatgaa aatgagtctg
    15721 agtttatacc aagtgaagag tgcataaaca aaagtaaaga ttttttaatt aagctaaagg
    15781 aatttaaaga aaagtcaatg tacatgagta cagatgaatt tatatggtac ttgtacacaa
    15841 ggactggata ctatgcctat gttggagcat tgccaggtgg aagtcaaaga caagctaatt
    15901 taaaagtatt gtttgagaga gcaaaacaat ttgaagaaac cagtcttaaa ggaatattca
    15961 actttgtaaa ttttatagag aaattaaaaa aatctagttc tgatatggga agtgcaaaaa
    16021 cacttggtga gaatgcaaat gttgttagaa taatgagtat tcataaaagt aaaggtctag
    16081 aatttccagt agtcatatgc tctgcaatgg gtaaaaattt caatactcaa gattttaaaa
    16141 agagtatttt atatcatcat aatttagggt atggacctca atttgtagac tatgagagaa
    16201 ggatttcttt tccaagtata gccaaagaag cattaaaaag caaaataaat atagaaaatt
    16261 tatcagaaga gatgagagtt ttatatgttg catttacaag agctaaagaa aaattgatta
    16321 taactggttc tactagaaat atacaagata gtataaagag atggtcaaat ggaattgaaa
    16381 gtttagatac aatatcacaa tacgagatat taaaaggtaa aaatttttta gattggataa
    16441 tgccttgtgt attaagacat agggatttat ctaatctact agaagaagta ggattagacg
    16501 cagtttttaa tgttgaacat aattcaaaat ggtatggaaa gttgtggaat aagaatgata
    16561 ttttagtgga aaagaagagt gatgaagaaa aagaaagtat tgaagaaatt cttgaaaaaa
    16621 tagatgtaaa taatcctgac agtgattatt acggtgaaat agaagaaaaa ttaaactata
    16681 tatatccata tgaatttagt acaagaaaac ctgctactat atctgttaca gaaataaaaa
    16741 agatacaaaa taattatgaa gaagagttaa taaatactat atttgaacaa aaagtaatat
    16801 taaaaaagcc tctatttatt caaaatgaag aagaaagaga aaaaataagt ggaacagaaa
    16861 gaggtacaat agtccattta gttatggagg ttttagactt aaaaaatgta agtagtgtaa
    16921 atgatataaa atcacaaata agaggttttg tttcaaaagg tataataaca gaaaaacaag
    16981 ctagtatagt aaatccatat aaaatatata aattttttgc ctctaacata ggaaaaagaa
    17041 tgttgaatgc tgaaattata aatagagaaa aatcaattta tgcacaagta aatatgaaag
    17101 acatatatat ttatgaaaag cttataaaca atgacgataa aaaactttat gataatgaaa
    17161 gtgtcatgtt aagaggtata gttgatgctt attttgaaga agataatcaa atagttttgg
    17221 tggattataa aacagatttt gtaaatgagg aaaatataaa tcaaataata gagaaatata
    17281 aaaaacagtt ggatttatat gcagatatta ttgaaacttt aactggtaag tcagtaaaag
    17341 agaaatgtat atatttattt ggagttgatg aagctgtttg ttattagttt ttatatataa
    17401 attaaaccta aaagatagag gaaggggaaa aaatgagatt tattcataca tcagattggc
    17461 atctagggaa gtccctagaa ggccattcta gaattgagga acaagcaaag ttttgtgagg
    17521 aatttataaa aatagtagaa tcaaatgaaa tagatatggt tataattgct ggagatgtat
    17581 atgatacttc taatccccca gcacaagcag aaaaactatt ttatcagact gtatcaagac
    17641 ttgccaataa tggtcagagg tgtgtattaa taatttcagg aaatcatgat aatccagaga
    17701 gactctctgc aataacacct ctagcacatg aacaggggat acttatatat ggttatccat
    17761 taagtgctac gatagaagct aagtataaag ggtttgaaat aacatatgca actcagggat
    17821 gtacaaaact gaatataaat ggagaaaata ttgtggttgc tacattacct tatccaagtg
    17881 aaaaaagatt aaatgaagtt ttttcaagtg aagatgagtt tgagaaacaa aaaaattatt
    17941 ctgaaaaggt aggagatata tttagaagtt tagaagaaaa ttttagaagt gatactataa
    18001 atatagccgt atcacatata tttgtaatag gtggagagag tactgaatca gaaagaccta
    18061 tacagttagg tggaagtttt ttagttgaaa gaaaagattt gccagaaaag gctcaatata
    18121 ctgcactagg tcatttacat aaacaacaaa aagcttccga acgtttaaat gcatactatt
    18181 ctggttctcc acttcaatat agcaaggatg agagagcgta taccaaaggg gcatacatag
    18241 tggatataaa agctggtgaa aaaccaataa ttgaagatgt atattttaat aattacaaac
    18301 ctatagaagt atttaaatgt aatggaatag aagaagcact agatatatgt gagcaaaatc
    18361 aagatagaga gatttggtca tattttgaaa taaatactga tgagataata tcacagaatg
    18421 aaattaaaaa aatgaaggaa ctattaaaag acataataga aataaaacca ataataacat
    18481 cttgttatga acaagaaagt gtagatataa aagaaaagtc tatggcagag ttgtttagag
    18541 agttctattc ttttagtaaa ggtgtagagc caaaaggaga acttatggat ttatttctag
    18601 atataataag tgaagaaggt gaatcagcag atgagaccaa ttagattaga acttacaggg
    18661 cttaatagct atattgataa acaagttata gactttgaaa aattaattga aagaggtttg
    18721 tttgggatat ttggaactac tggaagtgga aaatctacaa tcttagatgc aataactatt
    18781 gctatgtatg gaaatatatc cagaaataca aaagaatata taaacagtgt gtgcgataag
    18841 gcaattatat catacgagtt tgagatagga agcaaaaaca ctagaagaag atacattgta
    18901 gatagaacca ttgctagaag caaaacagga acaaagacct ctaatgctag attaatagaa
    18961 gtattgaatg acaataccca aaatgtttta gctgataaag ttgtggaagt aaatgaaaaa
    19021 gtagcacagg ttgtaggatt aacagcaaat gattttacta ggtcagtagt tttaccacaa
    19081 gacaaattta atgagtttct aagattatca cgtgcagata gaagagacat gttagagaga
    19141 atttttaatc ttgagaaata tggaagaagt cttggagaaa aagtgaaaaa gagaaaaaat
    19201 atacagttac aaaatttaaa agatttgaaa tcaaaattaa gtcaatatga tggaattaca
    19261 gaggaagtat acaacaatat aaaccaagaa ttaatagagt taaaaaattt ggaaaaagat
    19321 aaggataatg ctttagacct agctcaaaaa tcttatgaag aaagtaaaac agtatatgaa
    19381 gaacaattaa agttagaaaa aaatgaactt agaaaaaatg agcttgattt aaaaaatagt
    19441 gagataaaag aaaagattgc tcttgtagaa aatgatagta acgctaaaaa ggtagaccca
    19501 tacataagtt cagttcaaaa tctagaaaaa aagatagatg aagatagctt cgcagtaagt
    19561 aatttagaaa agaaattagc aatattaaat caggagcttg aagttacaaa aaatagatat
    19621 gaaaaaataa acaaaattaa gaatgaagaa gtccctaaat tgagtgaaga aaaaataaga
    19681 ttgcaacaag caataaaatt agaagaagag ttagttttat tagatagaga gttaaaagag
    19741 ctaaaagaaa gtggaattaa tttaaataag actaaagttg aattagaaaa agtaaagcag
    19801 gtgtcagagt ccagaaaaga tgcagttact aaatctataa aagaagttga aggaaagatt
    19861 gataaagtaa atataagtgc agaattaaaa caaaaaatat ttctagcata tgaatacgag
    19921 aaagattaca acaacgtatt ggaagaaaaa aatcaaaagc taaataaact agaagaaata
    19981 ctcaaagaga cagaaaatat aaatttaaaa gttagatata ttgataaaga taagaatgat
    20041 gtaaatcgta atttagaaaa tttatcactt cacttggatg ttcttcttaa aaaatgtcct
    20101 ggtaagagtg ctgatttact tttaaaatct gaatatgtta ctgaattaag aaataaagca
    20161 aataatacta aagaaaatga aattaagaaa agcagtatac aagatgaatt aaagataata
    20221 cttgaaagta agtttaatac tgaaagggaa gtaaatttac taaatgaaaa acttgaaaat
    20281 aatagaaaga acagagatga cttagaaaaa gaattagaag aactaaagta tttaaattta
    20341 gcctctgaac ttagacgtga attaaaagaa aatatgcctt gtccagtttg tgggtcaaaa
    20401 catcatgaaa atcatgatat aactaagtat gatgaaaata tatcttttgt caaagaaaaa
    20461 ctggagaaat tagaaaaaga aaaaataagt ataagaaaca atatagagga attaaatgct
    20521 aaagtaagtg ggtatttatc tatagaaaaa atgaagacta aagaacttga agatgtaaaa
    20581 ggaaaactag gggggattcc atctagtcaa ttattaaaaa aacttgatga agaacaaaga
    20641 aaacttgcat tgttaaaatc taatattcag gagtgggaga aagaaaaaga gagtactgaa
    20701 aataaagtaa cattagctaa agaagaaaaa aataaaattg aaaaagaaga attaaaaata
    20761 agagagagtc tgaataatta taagaaatta acaagagatt taaacataga aatagaaggt
    20821 ttagaaaata aatgtaaaaa acttaaacag gaatacctag gattaaagac gattactaaa
    20881 gtctctaatc tattgtcaaa agtaaaagaa ataagagaga atgaaaaaga attagaatta
    20941 ctaagtactg ggcattcaaa tttattaaaa aatagagact cactagatat taaaataaga
    21001 gaaaatgaga gtaaactaca tgaaatagag atagaattaa taaaggctag agaattatat
    21061 gtagaaaaga aattgagtag agataataag tataaagaag ttattagcat aacgaaaggt
    21121 gatttagcta aaaatctttt acataatgtt gaagaaaata tatgtaagat attagaacaa
    21181 gaagaatcaa gtaagaaaaa attggaagag caaagattag aatatgaaaa aaacattgct
    21241 gaaaagcaca atatagatgg tagacttaaa actgctaagg aacaatataa agaccaaaaa
    21301 tcaacactaa ataaactatt agcagaaaat aaatttgaaa gtatatatgc agtaaaaaga
    21361 gctttattag atactgatgc agtaaaaaaa ttgattgaag aaatactaga gcatgaagaa
    21421 gaacaaaagt tattatcatt taaaataaaa tcatcaaaag aaaaattaaa tggaagaagc
    21481 ataaagaagg agtactttga acagttaaaa gatgaaattt ataatttgaa agtagaaata
    21541 ggaaatatat ccaaagatat tggagctaat caaaatcagt taataacatt aaaagactct
    21601 ttagataaga taaatgattt taataaacaa cttaaggtag ttgaacacaa tgtggactta
    21661 ttggaagaat tagataaatg tgtacaagga aataaatttg tagaatatgt ttctacaaac
    21721 caattaaaat atatagcgtt ggaagcatca aaacgattgg atggaataac aaaaggaaga
    21781 tatgcacttg aaattgactc aacactaaac tttgtaatga gagataactt taatggagga
    21841 gaaagaagaa gcattgatac tctatctggt ggagagacat tcttgacatc tctatcatta
    21901 gcacttgctc tttcatctca aatacaatta aagggaagtg caccattaga atttttcttt
    21961 ttagatgaag gttttggttc attagatagt gaacttttag aaattgttat agaatcattg
    22021 gaaagattgc atagtaataa tctaagtgtg ggtataataa gtcatgtcga ggaacttaaa
    22081 aatagagttc ctgttaagct attagtttca tctagtgagg ctggaatagg ttcaaaagta
    22141 aaaattgaat atagttagaa aatatatgtt aattgaatat attatctaat atttccaaat
    22201 aatatgtaat tgtgatataa taaaaactaa tattaaagtt atttataatt aatattaata
    22261 taaaaacatg taagtaggag tgtggtaagt gttggaatcg cccaataatt tgattcagat
    22321 tattttttta atagtattac ttatagggtc tgcatttttc tcagcatctg aaacggcttt
    22381 aatgtcgttg agtaaaatca gaatcagata tatgcaagat gaaggagtta aaggagctaa
    22441 gttagtaagt tcattaatag aaaatccaaa taagctttta agttctatat tggttggaaa
    22501 taacgtcgta aatatagcag caacatccat atcaacatct ttgtttatag gattaatggg
    22561 agaaaaagga gtggcacttg ctacagctgt tatgacagta ttagtattaa tatttggtga
    22621 gattactcca aaaacaatag cagcaaataa ttcagaaaag gtttctctat tagtatctaa
    22681 acctataaaa gcaattatat ttatattaag accaatagta tggatattta atataattac
    22741 aaatattata tttaaattat ttggtataac aaataaagga gctaaatcat tcataactga
    22801 agaagagcta aaaactatgg taaatgtaag tcatgaagaa ggcgtccttg aaatggaaga
    22861 gagagaaatc ataaataatg tttttgaatt tggagatatg caagctaaaa atgcaatggt
    22921 acaaagaata gatatggtag ctatagatat ggaagatagt tatgatgaga taatacaggt
    22981 atttaaaact gaaaaattaa gcagaatgcc tgtgtatgaa gaaactatag atgatattgt
    23041 aggaatactt aatataaaag atattatatt tttatctgat gaagaaatag agtcatttga
    23101 tattaaaaat tatatgagag aacctttttt cacgtatgag ttcaaaaaga taacacaact
    23161 tcttgaagag atgaaacttg aaaaaagtca gatggctata gttgtagatg agtatggtgg
    23221 aacatctgga cttttaacta tagaagattt agtagaagtc attgttggag atatagagga
    23281 tgaatatgat gaagaagagg atgaaataca agttataaaa gaagatgagt atattgtaga
    23341 tggaagtact aaaattggtg atgttaatga acttattggt gtaaacttgg aatctgagga
    23401 gtttgattcc ataggaggtt ttataattgg acacctaagc agattgccag aagaaaatga
    23461 agtaattgag gttgataata taagattttg tatagaaagt atagaaaaaa atagaattaa
    23521 aaaaataaga atatatacat aatgtaataa tgttcttaaa tttattagaa gtgtataaat
    23581 ataaaagata tcttttatat ttatacactt ctttttattt tgtattcaaa taaaactagt
    23641 atgaaatgga caaaataaaa atataaatta gaagaaagga gatttttaag atgagcatac
    23701 tagaaaataa ttttttatac aaattaaaaa acaacactat ctttttttgt atatatacat
    23761 tgttatttat ttttatttac aaagcgtttc catacatagc tccatttttt ctaggtgcta
    23821 taatagcact tatgattaat cctatatcac aaaagttaga aaataagttt cgtataaata
    23881 aaggtatctc tactcttgtt ctaagttttc ttgcagtggc tatagtcagt acagttacta
    23941 caataatagt aataaattca atgaaagaat taatgggatt tttaaataat atatctgcta
    24001 atcctgagga tataagtaat actattatgt attttttaaa taaaataaat gactttatga
    24061 aatcatttca agaaatagcc aattttgatg tagaacaatt agtaaacaaa ttcagtggtg
    24121 aagtaatgca aattacaaaa aatcttctta ctagtatact tggattggct acttctattc
    24181 catatattat aatatttata attaccttgt ttattgctac gtactttata gcaaaagacc
    24241 ttgataagat tgaaaatagt ttttataata tgtttactgt tgatgttaga aagaaggtaa
    24301 aaaacgtaaa gaaagaggca gggctttcac ttgttggata tataagggcc tatacaatac
    24361 ttatggctat aacatttttt gcaatctggg gaagttttgc tttatttgga ttgaaatacg
    24421 gtctaatagt agggtttgta ggagctttaa tggatttaat tccattccta ggtataataa
    24481 caatatatct tccagtaatt gtttattatt tccttataaa aaattatttt atagctataa
    24541 gtatgactgt gatatttttt gttttgtctt tgattagaga aatacttgaa cctaaactag
    24601 tatcagtaaa tgttggacta aatccattag ctaccctagc agcgattttt attggaatcc
    24661 aagtaaaagg aataattgga gtaatttttt gtttgggttt ggtatgtatg catgatatac
    24721 ttaagaaggt ggatattttt taattataat aattgataga ttttttaaaa ataaaatttt
    24781 attcacaggt aaataaaaaa atattaatct tattaagaaa gagggtatat acgtgaaaca
    24841 aaaagtagtg gaaagatttt taaaatatgt atcatttgat acaacatcta attctcagtg
    24901 tgagaactgt ccatcttcag aaggtcaaag agttttagct aagtatatag ttgaagagct
    24961 aaaaacaatg ggtgttgatg atgtgagttt agatgaaaat agttatataa tggctacttt
    25021 aaaaggaaat actgatggtg tggatactat aggattcata tcacatttag atacaattga
    25081 agatgttagt ggaaaagata ttaaaccaag aataatagaa aattatgatg gaaaagacat
    25141 agttttaaat gaagcgttaa atgttattac gtatgtaaag gattctccag agttggaaga
    25201 atttaaaggt gacgatttaa tagtcactga tggaacaact ctacttgggt ctgatgataa
    25261 agctggtata gctgagatag tgactgcaat agagtatcta ataaatcatc cagaaattaa
    25321 gcatggagat ataaaaatag gatttacacc agatgaagaa attggtagag gtgctgattt
    25381 atttgatgtt gaaaaatttg gagcaaaata tgcatatact ttagatggag gaatagttgg
    25441 agaacttgaa tgtgaaaatt ttaatgctgc aaatgcaacc attactatac atggtagaaa
    25501 tgttcatcca ggttcagcta aaaataaaat ggtaaatgct atacatatag ctgctgaaat
    25561 atcagaaatg ttcccagcag atgaaagacc agaaacaaca gaaggatatg aaggattttg
    25621 gcatttaaat tctataggtg gaaatgtcga gaatgtaagt atggcttata ttattagaga
    25681 ccattgtaaa gaaaaatttg aaaatagaaa atctattatg atagagaata tagaaaaaat
    25741 aaataagaaa tacgataata gagtggaatt ggatttaaaa gactcttatt acaatatgaa
    25801 agaaaagatt gagccagtta tgtttatagt tgatatagct aaagaagcta tggaggaatt
    25861 aggtataaaa ccacgtttag ttcctgtaag aggtggtact gatggagcaa gattatcttt
    25921 taatggatta ccttgtccaa atatatttac tggtggatta aacttccatg ggaaaaatga
    25981 gtgtatacca gttagttcta tggagaaagc tactaaacta attgtaagaa tagctgaaaa
    26041 atatgctgaa agagtttagt taaaaagtat ttaaaaatta agcatttaaa gttataatag
    26101 ctatatcatt ctttataagt caagctgatt ataagcttgt gttgtactta aaatggtata
    26161 gctatttttg taattatttt tataaaagat aattattttt agtttagggt atcaataaaa
    26221 agtatgaata tcaataggta ttaacattta aaagggggaa ttgtattgaa aattaaatct
    26281 aaaaaaataa tagcatttgc actttcactt gggattatgt tgccaagttg tgtatatgca
    26341 gacactatta gtaaaaatca aaatgcacag gtagatatga acaattatct tcaaggtaga
    26401 aaatctccaa aggaggaaaa acttattggt gaagatagat ttgatacagc gataaaaata
    26461 tctcaaagtg gttggaataa tggttcagaa agggtgtttc tagttaactc aaattcattg
    26521 ccagatgcat tggcttcaac tcctttggca agtaaattgg atgcaccaat actgcttaca
    26581 aataaaaata gtataccata taatgtaaca gaggaaataa aaaggcttaa tccagcagaa
    26641 gttattttaa ttggaagtga aggagctatt tcatcaagtg taaaagaatc tcttgaagat
    26701 atggaagtat tagtcagtag aatcggtgga gcagatagag aagaaacatc tttacttctt
    26761 actagacagc tagatgatac tggtgattta ggtgtctcaa aagtggcagt ggttaatggg
    26821 tataatggac tggcagatgc aacttcaata tcaagcccag ctgcaagtga taatacagct
    26881 ataatatata caggtaagga tagcataaga agtgaggcaa agaattttat tacacaaaac
    26941 tcaactaaga catacatagt aggtggggag tacagtataa gtaaaaaact tgaaggacag
    27001 ttagtaaact cagaaagact agcagggaca gatagaaaag atactaatgc gaaagtttta
    27061 gaaaagtttt atggaaaatc aagtaaggtt aataatatgt attttgcaaa agatggaagt
    27121 ggtagagaag ctgatttagt tgatgggctt gctgctggag tattagcatc aaaaacaaag
    27181 tcacctgtag tacttgcaag tggaagttta agtagtgcac agaaaagttt tataaaaaaa
    27241 gtaaaggctg ataaatttgt acaagttggt ggaggtaaaa atagtaaacc atatgcagaa
    27301 gcattagcct tacagtaata ttgtgtaaat caaaattaat atataataat agcaagtaaa
    27361 ccaagttgca cttcaatata tataataaaa taagtaaact aagctatgtt tcaatataat
    27421 atataataaa ataagta
//
LOCUS       DAMJYR010000041        27285 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.041, whole genome shotgun sequence.
ACCESSION   DAMJYR010000041 DAMJYR010000000
VERSION     DAMJYR010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 27285)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27285)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27285
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.041"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            245..778
                     /gene="pyrR"
                     /locus_tag="PC471_001761"
     CDS             245..778
                     /gene="pyrR"
                     /locus_tag="PC471_001761"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001156491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HDF2788892.1"
                     /translation="MVEKAQLMDEKAIARAITRISHEIIERNKGVENLVLVGIKTRGV
                     PIANRISKKIEQIEGTKVDTGDIDITLYRDDLEKIHVEPVVKGTYLDFNVNDKTVVLV
                     DDVLYTGRTVRASLDAIIDIGRPKSIQLAVLVDRGHRELPIRADYVGKNVPTSRHEII
                     SVSLLEIDGEDSVTIKE"
     gene            1062..2273
                     /locus_tag="PC471_001762"
     CDS             1062..2273
                     /locus_tag="PC471_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HDF2788893.1"
                     /translation="MKKTIMSLQHLLAMFGATVLVPILTGFNPSVAIFCAGVGTLIFH
                     FCTEGKVPAFLGSSFAFIPVILAAKEAYGGDLAYAQGGIIVAGLIYIIMSIIVKVVGV
                     NKIKLYFPAQVTGAMIVVIGLNLLPTAFSMASANFVIAFITLAIAILTNKFGRGFIKQ
                     LGILIAVFSGYIICLILRLVDITTITEASLFAIPNFTVPKFSLGAIVIISPVVLAVFM
                     EHIGDMTTNGAVVGKNFIENPGLNRTLLGDGFATIVAGCLGGPANTTYGENTAVLAIT
                     KNYDPSILRRTAIFAILLACVGKFGGFLQSIPGSVMGGISIMLFSMITYVGLKTIRDS
                     SCVESKINILIIAVILLIGLGTTYLSNKGISVGIPITSTVKITGLSLAAIVGIVLNRI
                     LNNQDFKVEEE"
     gene            2572..4224
                     /gene="abc-f"
                     /locus_tag="PC471_001763"
     CDS             2572..4224
                     /gene="abc-f"
                     /locus_tag="PC471_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein"
                     /protein_id="HDF2788894.1"
                     /translation="MLLVKVENLKKYYADKLILDIDKLEILENDKIGLVGSNGQGKTT
                     LLKAILGEIEIDEGYTYLTESYSYISQSENNIETCSHSKEKSLLNAPDKFEEHLSGGE
                     KVKLKIADALSNKKNIIIADEPTSNLDKKSIGVLEDMFKRHEGALLLISHDRRFLDEL
                     CTTILELEDGKLKAYKGNYTDYLMQKDEEVKRADFEYQEYVKEKKRLEKALLYKKALS
                     DGIRKTPKRMGNSEARLHKMGGQTNKKKLDSNVKAIKSRIDKLEVKNKPKVSKEMNIK
                     IQDGMEIISKNLVEVKDMTLKLENKLLLDNVSFKIKRGKKIALLGDNGCGKSTLIKEI
                     LADKNDNIKINNKVKVGYFDQNQSLLDEEKSVLYNTKVNSSFDESFIRINLSLFGFKG
                     DDVYKKVKVLSGGEKVKIALCKIILEDNNFLVFDEPTNYLDIKSMEALEKALINTDKT
                     MLIVSHDRVFVSHICNYIIEIKDAKIREFDCNYDEYTISRNKKTPSRENQIKKENLLV
                     LENRLTTVISMLSIEKDNLKKELYESEYNELLKQINKLKNSF"
     gene            complement(4439..6214)
                     /locus_tag="PC471_001764"
     CDS             complement(4439..6214)
                     /locus_tag="PC471_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NFACT family protein"
                     /protein_id="HDF2788895.1"
                     /translation="MALDGLVIHSIVDELSSKLTGGKIDKIHQPEDDEVIFNIRNNKE
                     NFRLVLSASASNPRVYLTSNYQKENPLKAPMFCMLLRKYIQGGNIVEISQIGFERIIK
                     ISVESLDELKEKTVKNIMIEIMGRHSNIIITHGEENKIIDSIKRVPFSISRVRQVLPG
                     HDYSLPPEQNKLNPLDDISKDLFIKNLEELEGPIFKSIYSKFLGISPIIAKEICYRAG
                     VNQNAIIKDISDEQFDSLHKVFCNLFNDINSNKYSPCIIIDKKVDKVVDFSCINLTLF
                     SDLSYINKDSMSRILEDFYRTKDIKDRINQRSSDLKKSISVKLDRLYNKLKKQEEELS
                     ESENADIYKIKGELITSYIYMVEKGMESIEVANFYDENCNDVTIELNKNLTPSENAQK
                     YFKKYNKMKHAKVEISHQISLNKEEIDYLENIILSIENCENLAELQDIKEELAKVGYI
                     KTQKKNSKKDTIPSTKPHEFVSSDGFKILVGKNNKQNDYLTLRLADNDDLWMHTKNIP
                     GSHVIIKCAGKEVPDNTVFEGAMLAAFFSKSKLSSQVPVDYTKRKNVKKPSGSKPGMV
                     IYETNSTIYVTPEEETVAKLKVKSE"
     gene            6510..7544
                     /gene="ltaE"
                     /locus_tag="PC471_001765"
     CDS             6510..7544
                     /gene="ltaE"
                     /locus_tag="PC471_001765"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HDF2788896.1"
                     /translation="MRFIDLRSDTVTMPTDEMREAMAKAPVGDSIFRDDPTVNKLEEL
                     AAAKVGKEDAIFLPSGTFGNQLALFTHCLRGQEIIIGKGYHIVTHEVGAPAVIAGVQL
                     RTIDEDESGALNPELVEKAIRRDDIHEPQTGLICVENAYSGGTVVSLDNLREIKKIAE
                     KHNLPVHLDGARLFNAALTLGVEAKEIAKCCDSVMFCVSKGLAAPMGSILAGNKEFIA
                     KARRKQKVMGGGMRQVGIVAAAGIIAIEKMTLRLNEDHENAKYLACELNKIDGIEVNN
                     VNPDISMVFFKMSEDIIKEDVLIKEFFERNIKINKMENGEYRFVTHVDITKEDLDYVL
                     KTLKELIGVC"
     gene            7831..8661
                     /locus_tag="PC471_001766"
     CDS             7831..8661
                     /locus_tag="PC471_001766"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HDF2788897.1"
                     /translation="MKFWKLHGIGNDFIAIDGRFDQINPSDYTALAKKVCHRRFSVGA
                     DGLLVVKDSKVADVEMVYYNSDGSRAAMCGNGLRCFCKFVYDNCIVKKESFTVDTLDG
                     IKDIKVNLTNREINSIRVNMGKGSFIAKDVPVLTNKERFVQESIKVLDRELKVTSMLM
                     GVPHTVVIVDELDVDDVCKYGKEIENNKIFPEKTNVNFVKVEDKYNIHVYTWERGCGY
                     TLGCGTGMTASAIVCNLLNMVESSVNVTSQGGTVKIDVGDFSYMTGPAVKICEGILEV
                     "
     gene            8665..9546
                     /locus_tag="PC471_001767"
     CDS             8665..9546
                     /locus_tag="PC471_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HDF2788898.1"
                     /translation="MAISMTGFGRGEYKDDNYYFLVECKTINHKYSDINIRLPRKISF
                     LEDKVRNLVKNYVKRGRVDLYIKFDLLGKEDVNLNFDEGLASQYIDILKEIKNKFDII
                     DDISVMNVAKFPDIVKIEEKEEDEDLLWSMLNQAVEDALIKLREMRSEEGKKLAEDIA
                     MRCDLLKNHIEEIEKYSSSVVEDYREKLNLRISELLDDPSIIDENRLAQEVAIYADKS
                     SITEEIVRFKSHIGQLKNTIFKDDSIGRKIDFLIQEMNRETNTIGSKSSDINITNLVV
                     EVKSELEKIREQIQNIE"
     gene            9576..10193
                     /gene="gmk"
                     /locus_tag="PC471_001768"
     CDS             9576..10193
                     /gene="gmk"
                     /locus_tag="PC471_001768"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HDF2788899.1"
                     /translation="MFVKKGLLLVVSGPSGAGKGTICKELLKENDTIKLSVSATTRKP
                     RTGEVDGVNYFFISKEKFEEMIEKGEFLEYAQIYDNFYGTPKAAIMECLEKGQDVLLE
                     IEMQGAKQIKEVCPEGVFIFVLPPSLKELKNRIVGRGTETEAEIEKRFSCAYEEIKMI
                     KDYDYFIFNEDVKTSAKEIEGIISSEKNKVSRYKNIIIEKFKEEL"
     gene            10196..10462
                     /locus_tag="PC471_001769"
     CDS             10196..10462
                     /locus_tag="PC471_001769"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HDF2788900.1"
                     /translation="MLKPSINEVLEKIDNRYYLVGTVSKRARKLIDGEEPYVSNKTKE
                     KPVCVATKEVASGKITYRLLTEEEIEIEEARHHAEQHQQISEEE"
     gene            10467..11666
                     /gene="coaBC"
                     /locus_tag="PC471_001770"
     CDS             10467..11666
                     /gene="coaBC"
                     /locus_tag="PC471_001770"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HDF2788901.1"
                     /translation="MLKDKTVVIGISGGIAAYKACDVVSKLKKLNANIHVIMTKSATE
                     FVKPLTFQSLSQNYVVEDMFEEPKTWEVEHISLAKKADLFLIVPASANVIGKIANGIA
                     DDMLTTTVMATKAQVLIAPAMNTNMYENPIVQKNIQTLKSFGYKFIEPESGRLACGDI
                     GTGKLANPDIIVEEVLTNLTKEQDLKGQSMIVTAGPTVESIDPVRYLTNRSSGKMGYS
                     IAREAIARGADVTLISGPTNLSIPQNLKKFVQIESAQDLYEAVVSNLSENKIVIQSAA
                     VADYKPKTYSDKKIKKKNDDLSIELSRNYDIAYEIGKIKEDKILIGFAAETNDLIEHA
                     KGKIKKKNLDFIVANDLTKEGAGFRADTNIVKIIDVEGNITEYPKMKKEEVANVILDK
                     VKALLEK"
     gene            11905..14430
                     /gene="priA"
                     /locus_tag="PC471_001771"
     CDS             11905..14430
                     /gene="priA"
                     /locus_tag="PC471_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HDF2788902.1"
                     /translation="MNNILNLSRGFIMKKYAKVIVRSNTIYTDNLFTYQIPVFLSDVI
                     KIGHRILVPFGKGNKPTEAFVFQFTDSLDEKIKIKEIIDILDENPIFRKEDLELVYWM
                     KNRYLCTYIECINLIYPKGYKLNNYKVVLLGESLSGLNDIELKEKISTLSDKNREIVE
                     KVIDSKGKIKVDKLKYIPNLNSSLYTMNKNGIIKLCWEYKNHKNEKKVCYISLSLESD
                     KIDDYIEQNKINVGSKQKEILSFLKNNENVEINDLLDLLNASKQSINSLSKKKLITLE
                     FKDYYREPKSIYKSVLKSIKLNNEQQEAVDEIKSNMFVDDKKPYLIHGVTGSGKTEVY
                     MEIIEFALNQGLDSIFLVPEIALTPQTIDRLKSRFGDLVGVFHSKLSEGEKHDVYKAV
                     KAGKVRVLIGARSALFAPFNSLGLIIIDECHESSYKSEKNPKFNAIEVARFMALKNNI
                     TLILGSATPSIEEYYRAKSGEYKLINIKSRANDKPLPNIEVVDMKDELDKGNRSIFSL
                     KLQKEIRYAIEENNQVILFLNRRGYANFVSCRKCGYVFQCENCDISLTYHKKSNTGRC
                     HYCGYEKEIPKECPECKSTYVKPFGVGTQKIEEELKYIFPDIKTLRMDKDTTSKKGAL
                     DEILNKFKDKEADVLIGTQMLSKGLDFENVTLVGILSADMILNFPDFKSFETTFQLIT
                     QVSGRAGRADKEGKVVLQTYDTEHYAIKHAIEYDYEGFYEDEIKIRKAFGYSPFNNML
                     SVVVSGEDERLVIKNIKNMHASLIYLLEKRGINDLGFILGPNPCSISKINQNYRWQIL
                     FKDENIEINLLKGIIKYICITKRDLIFDKNINVSIDINPNSVL"
     gene            14448..14888
                     /gene="def"
                     /locus_tag="PC471_001772"
     CDS             14448..14888
                     /gene="def"
                     /locus_tag="PC471_001772"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HDF2788903.1"
                     /translation="MALRQIVQIGEPVLRKKSKKVEKIDEKIIQLLDDMAETMYDADG
                     VGLAAPQVGILKRVVVIDIGEELIELINPEIIETSGEQIDEEGCLSVVGEAGNVRRPN
                     YVKVRALNRNGETIELEGEELLARAFCHEIDHLDGILFVDKIEK"
     gene            14903..15832
                     /locus_tag="PC471_001773"
     CDS             14903..15832
                     /locus_tag="PC471_001773"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HDF2788904.1"
                     /translation="MKIVFMGTPDIAVPCLQKIIDEKYEILGVVTQPDKPKGRGKKLG
                     MSPVKELAIENNIPVYQPVKARDKEFIDKIKSLNPDVIVVVAFGQILPKEILEIPKLG
                     CINVHVSLLPKYRGAAPINWVIINGEEKTGVTTMYMDEGLDTGDMILKTEVNLDENIT
                     AGELHDKMMNIGAETLKETLRLIEEGNAPREVQNHEEFSYAPIMNKSLGNIDFSKSAR
                     EIHNLVRGVNPWPSAYTTYNDVIMKVWKTKVLDEKSTKDVGTIIDVSKDGIKVSTIDN
                     VLLIEEIQMPNKKRMLVGEYIKGNTIETGLVLG"
     gene            15842..16594
                     /locus_tag="PC471_001774"
     CDS             15842..16594
                     /locus_tag="PC471_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF116 domain-containing protein"
                     /protein_id="HDF2788905.1"
                     /translation="MTDIKKYLTSVGILILILGILIGVVNFFIISLTQIFSLMINIII
                     ISLILIILCSTAVTYRVIKGKYVNSNIMKINFNIVSGLFPIISFIASSFGMSKSDIRR
                     IYIKLNNEYIYSNKYNFNPEDIIILIPHCIQENSCKLKVTNDIDNCKECGRCNIGELI
                     KLKEKTNVKIFVATGGTLARKIIMDNKPKAVVAVACERDLTSGIQDIKKIPVLGVFNK
                     RPNGPCVNTNVDIMDIEKAIGFLTGKNILVCN"
     gene            16663..17364
                     /locus_tag="PC471_001775"
     CDS             16663..17364
                     /locus_tag="PC471_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HDF2788906.1"
                     /translation="MYPVYGGFWGFDPTMVVLIPAILLTIYAQFKVSSTTNKYLRVNT
                     RRGYTGEQTARRVLDSNGLYDVKIEMVRGHLSDHYDPRRKAVRLSEDVYYGTSITSVA
                     VAAHECGHAIQHAKGYAPLQIRSSLVPVVNFASSISWFLIFLGFIMAGPFLKIGILLF
                     SASVLFQIITLPVEFNASSRAIVQLGNLGIIDESESRQSRRVLSAAALTYVAAALVSI
                     LQLLRLLLIAQRRND"
     gene            17426..18751
                     /gene="rsmB"
                     /locus_tag="PC471_001776"
     CDS             17426..18751
                     /gene="rsmB"
                     /locus_tag="PC471_001776"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HDF2788907.1"
                     /translation="MDAREVGFKVLCDIEKNDNYSNIAINKHFKNLEISDMDRGLATE
                     LIYGVIENKYYLDYIINKLSKIKVKKMSTYVKIFLRMGTYQILFLDSISDYAAVNETV
                     KLSKKYDKKSSGFINAILRNEIRAKETIMDITEEDSVKYLSIKYSYNSWIIKNWIDKF
                     GQEFAEDLLEANNEKPSIYIRTNTLKISREELIEKLSEMGIVCLKVPMVEEAIKVEKM
                     KNIEKNELFKAGLFTIQDISSMIVGKVINPKEGSFVLDVCSAPGGKSTHLATLMNNTG
                     QVVARDIFEHKLKLINSTVNRLGLKNVFVERFDASEIDENSISKFDYVLADVPCSGFG
                     IIRRKPEIKYKKEEELKDITSIQKKILENASKYVKVGGTLVYSTCTIQDVENINIVTS
                     FVEENHNFEFVPIDTVNVDLDNQDKGYLKIYPNIHGIDGFFIAKLKRIR"
     gene            18759..19790
                     /gene="rlmN"
                     /locus_tag="PC471_001777"
     CDS             18759..19790
                     /gene="rlmN"
                     /locus_tag="PC471_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HDF2788908.1"
                     /translation="MEKKKIVLKNFTEDELKEFMKTIDEKPFRGSQIFSWIYKGAKTF
                     DDMNNIPKSLRNKLEEVSCIGHIDIELKLESKVDNTKKYLFLLDDGNIIETVMMDYDS
                     RVTVCVSNQVGCRMGCNFCASTMDGLIRNLEPWEILDQVIKIQEDTGKRVSNLVLMGS
                     GEPLDNFENTKQFLKIINEKNGLNIGYRHITLSTCGIVPKMYELADLEIAINLALSLH
                     SPYDEERRKIMPVANAYSIEEILNACRYYIKKTNRRVTFEYSLIKGVNDSEKEAKALA
                     KLLKGMLCHVNLIPINKVEEREYEKPDKAFIYKFRDSLEKNNIPATVRMSMGSDISGA
                     CGQLRRKYK"
     gene            19810..20562
                     /locus_tag="PC471_001778"
     CDS             19810..20562
                     /locus_tag="PC471_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HDF2788909.1"
                     /translation="MVYSCASHIGKIRKNNEDYCEGEVIDTEHGPIGIFAIADGMGGH
                     KKGEVASKLAVENIIDFLKENLLQHDNVKIDYIDDILKQAYNNVNSIVHKKSMEDIEF
                     EGMGTTLVTAIVYNNVLYVANVGDSRCYLLTDEKFDKITIDHSVVEELMMAHVITEEE
                     ARRHPQRNRITRAIGTDDMVVVDIFKKEIKKSDIILLATDGLTGFIDDEDIRDLILDY
                     EYERTSNISEELISMANDVSGKDNVSVIVIKV"
     gene            20579..22594
                     /gene="pknB"
                     /locus_tag="PC471_001779"
     CDS             20579..22594
                     /gene="pknB"
                     /locus_tag="PC471_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HDF2788910.1"
                     /translation="MGDTILGNRYEIIRKIGDGGMAFVYEAKDRLLNRTVALKVLRPE
                     FVDDDEFLTKFKREAEAVASLSHPNIVNVYDVGEDGKVHYIVMEFVDGKNLKEIIQDE
                     GILDEYTALDITKQIAMALSAAHKKGIIHRDIKPHNILISNEGRVVKVADFGIAKAVS
                     NSTMTNIGSIIGSVHYFSPEQAKGKFVTNNADLYSLGIVLYEMLIGKVPFRGDSPISI
                     ALQHINDDIDFTSEEKVRIPQSVRTTIKKLTEKSSADRYQTAEELIEDIEYIEKNIDL
                     DFIKEYDDFATKKIDEKEINKVVNPTLAKPAPEKVVKPVEVADLDDDEDYYDDFYEED
                     DDEDEEEEEIMRAKKNQRPKSTPSKRTKKKKKKQESPKSRRRLKVIAAVLILILCAQV
                     FLAYKFLFAGGFGNKSLTVPNLVNMTLEEAQSAVEKEGLYLSVKSEEYNSEVDENCII
                     SQTPEGGSTNVKKGDTINVVVSKGSSQASVPNVVGLTLSNAKQLIEENNLKVGTVKYE
                     YSSIYKEGTVLSQSPGAGSSRAQEGDEVNLYVSKGSEKSNTQTPTVPDKKPTTPENNT
                     PTEPGSNSGNSGGNSGGSNSGNSGGNSGNNGSNSGGSNSGNSGGNSGGSNSGDNGGSS
                     GGSNSGDNGGSSGGSNSGDNGDSSGGSNSGDNGGTSSGTGANIGKTE"
     gene            22702..23439
                     /locus_tag="PC471_001780"
     CDS             22702..23439
                     /locus_tag="PC471_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDF2788911.1"
                     /translation="MKKKTVLITGGARGIGKAMSKAFAKEGYNVLVNFNKSENEAKEL
                     YTILNEKNFSVKLFKADISNREDVEDMVDYCIKEFGGLDVLVNNAGVSQDKLFTDITD
                     EDWDNMMNINLKGSFYCSQVALKYMISEKKGNIINISSIWGISGASCEVHYSITKAGI
                     IGMTKALAKEVGPSNIRVNSIAPGVINTDMLSGYNEEDIDALVEETPLMRLGTPEDIA
                     NCAIFLASDKSNFITGQVISPNGGFVI"
     gene            23537..24421
                     /gene="rsgA"
                     /locus_tag="PC471_001781"
     CDS             23537..24421
                     /gene="rsgA"
                     /locus_tag="PC471_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HDF2788912.1"
                     /translation="MLEGKIIKGISGFYYVDTYNGIYECKARGIFRKQKITPLVGDRV
                     KISIVDENEKKGILEEIDSRDTELIRPPIANVDKALIVFAIKNPKPNLSLLDRFIVLA
                     EKENLETVIILTKADLDDNDTLETVKNIYELSGYKVIPVSNITKLNIDKVKEELKENV
                     VVFAGPSGVGKSSLLNEIDENFKLQTGVVSDKIKRGKHTTRHAELLKLEFGGMVADTP
                     GFSSLALEDIEEVELKDYFIEFDKFNDCKFGSKCIHENEPNCAIKEAVTNGEISKERY
                     DSYIQLLHEIRQNNSRRY"
     gene            24426..25097
                     /locus_tag="PC471_001782"
     CDS             24426..25097
                     /locus_tag="PC471_001782"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HDF2788913.1"
                     /translation="MIKLAPSILSADFAKLLEDVRKVESAGCEYLHIDVMDGHFVPNI
                     TLGPLVVKSLKKENINMVFDAHLMIENPDQYIEEFVKAGCDIITVHQEACVHLHRTIQ
                     NIKSHGIKAGVVLNPATPVDTIKHVLPDLDMVLLMSVNPGFGGQSFIPCVLDKIKELK
                     AIIDSQGLNIDIEVDGGISPKNVAEVVQAGANVIVAGSAIFGSDDIQETVNLFRKNAS
                     LEELV"
     gene            25106..25747
                     /locus_tag="PC471_001783"
     CDS             25106..25747
                     /locus_tag="PC471_001783"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HDF2788914.1"
                     /translation="MKICIVLNGEIEDYEVTRDIIIKECYDCIICADGGANHTYKMEI
                     MPDYILGDLDSVEEEKINFYKNKGVKFEKFPSKKDETDTELCLFLAKTLKANHIDFFG
                     ALGGRIDHTLANIKLLYYLKEDGIYSRILSDKEEMYIVENEEISLYGNPGDTISVIAI
                     NGDAKGVTLTGLEYPLDNYYMKYSVPIGISNVMLSNSCKIKVEQGCVLVVRNL"
     gene            25878..26507
                     /locus_tag="PC471_001784"
     CDS             25878..26507
                     /locus_tag="PC471_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HDF2788915.1"
                     /translation="MVDSIIFDLDGTLWDSTEEVCKVWQDVLSEHKEIELSVTKDLFR
                     SLMGLSFEEIANRLFPNLSEEERMNLLNECSLKECDYLSENGAKLFDDIEDTLKLLSK
                     KYKLFIVSNCQAGYIESFLKAHKLEQYFIDFECPGNTGLHKGENNKLIIERNKLINPI
                     YVGDTQGDANSAKFVGIPFIYAKYGFGNVDVYDYFIDSFKDLLEHDILK"
     gene            26562..27218
                     /locus_tag="PC471_001785"
     CDS             26562..27218
                     /locus_tag="PC471_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HDF2788916.1"
                     /translation="MELQDTIFKRQSVRKFKNQDVSDEDILKMIKAAGAAPSGKNIQN
                     WHFVVIKRRDLMEKIADVITKKQQEILVEMDKVSVDKANRFRKFVKNFTLFYLKAPVL
                     VLVFTKVYNPSGYYELELIDAPKETIDKLFIRNPGMQSLGAAIENFTLSAIELGYGSC
                     WLTSQNYAADEIEAVLEAETGFEKGEYFLGAMLALGVPEDNLKSPSKKPVEEICTFIK
                     "
BASE COUNT        10864 a         2768 c         5091 g         8562 t
ORIGIN      
        1 ttgaaatata aatatataat ttgagttgat agattaaaaa attatcttta aacgatacag
       61 agatatcgta agacaattgc taaattacac tgtatatttt ttatataaga gcttcttgtc
      121 ttgtgatgag aagttttttt gttgtttaaa ttgaaatttt aaataaaaat aaatttataa
      181 aaaattaaaa gaaaggagta gatttgtcaa atagatatat tatatctttg acaataatta
      241 tgatatggta gaaaaagctc aattaatgga tgaaaaggca atagcaagag caattactag
      301 aataagtcat gaaataattg aaagaaataa aggtgtagaa aatctagttt tagttggaat
      361 aaagactaga ggtgtgccaa ttgcaaatag aatttctaag aaaatagaac aaatagaagg
      421 aacaaaagta gatacaggag atattgatat aactttgtat agagatgatt tggaaaaaat
      481 tcatgttgaa cctgtagtta aaggaactta tttagatttt aatgtaaatg ataagacagt
      541 agttttagtt gatgatgttt tatacactgg aagaactgtg agagcttcac tagatgctat
      601 tatagatata ggaagaccta aatcaataca attagcagtg ctagtggaca gagggcatag
      661 agagttgcct ataagagcag attatgtagg aaaaaatgta cctacttcta gacatgaaat
      721 tatatctgtc agtttattag aaattgatgg agaagattca gttacaatca aagaataatt
      781 atttttaagt attatataaa agcttagcat tgaatagtca gggaggagga taaagcttac
      841 atagattgca caaaataaat attataattt tatggatgtg agtcttaatg tagtttttat
      901 tctcctttat ttgagttttt gatgtcgtaa aatgtaaaat aatgataaat tatataataa
      961 gtatatagag gttaaaaaat agaatctagg gaatgataat taattaaaag gtattaatta
     1021 aacagaggat ttatttatat ataataattt tggaggtttt tatgaaaaaa acgataatgt
     1081 ctttacaaca tctattagcc atgtttggag cgacagtatt agttcccatt ttgacaggtt
     1141 ttaatccgtc agttgcaatt ttctgtgctg gtgttggtac attgatattt catttttgta
     1201 cagaaggaaa agtacctgcc tttttaggtt caagttttgc gtttatacca gttatactag
     1261 cagctaaaga ggcgtatggt ggagatttag catatgctca aggtggtata atagtagcag
     1321 gattaattta tattataatg tcgattatag taaaagtagt tggtgtaaat aaaattaaat
     1381 tatattttcc tgcacaagtt acaggagcta tgatagtagt tataggatta aatttattgc
     1441 caacagcttt tagtatggct tcagccaatt ttgtgatagc atttataact ttagcaatag
     1501 caattttaac caataagttt ggtagaggat ttataaaaca attaggaata ttaatagcag
     1561 ttttttctgg gtatataatc tgtttgatat taagattagt agatataact actattacag
     1621 aagcaagttt atttgcaata cctaacttta cagttcctaa gttcagttta ggagcaatag
     1681 tgattatatc tccagttgta ttagctgtat ttatggaaca tataggagat atgactacta
     1741 atggtgctgt agtaggtaaa aactttatag aaaatcctgg attaaataga acattacttg
     1801 gggatggatt tgcgactata gttgcagggt gtttaggagg accagcaaat acaacttatg
     1861 gagaaaatac agcagtactt gctataacta aaaactatga cccttcaata cttagacgta
     1921 cagcaatatt tgctatattg cttgcatgtg taggcaagtt tggtggattt cttcaaagta
     1981 taccaggttc agttatgggt ggaattagta taatgctatt ttcgatgata acttatgtag
     2041 gattaaaaac tataagggac agttcttgtg tagaaagtaa aataaatata ttaatcatag
     2101 cagttatatt attaataggt cttggaacaa catatttatc taataaaggt atttctgttg
     2161 gaatccctat aacaagtaca gtaaagataa ctggtcttag tttagctgca attgttggaa
     2221 ttgtattaaa cagaatttta aataatcagg actttaaagt tgaagaagaa taaatttaaa
     2281 gaataagttg tatattgaat aactaaaatt agttaatttt ctatagacat ttgtaattaa
     2341 tgttgatata atgattaaaa ataaatgaaa gaaagggcaa ggtaactagg acaatgagaa
     2401 tataatctat tttttataat ttaaagtatt gtaatttttg aaaagcctat tttggtggct
     2461 ttatttgcaa tgcttaaatt aaggagagta gattatagtc tatacgttta tttgttatgc
     2521 ctatttatag aatctctcct tatatttaag tgtgttaagg agggattttt tatgttgtta
     2581 gtaaaagtag agaatttaaa gaaatattat gcagataaat taattttgga tatagataaa
     2641 cttgaaatat tagaaaatga caaaataggt ctagttggtt caaatggtca aggaaaaaca
     2701 acattattaa aagcaatatt gggagaaata gaaattgatg aaggatatac ttaccttaca
     2761 gaaagctatt cttatataag tcagagtgaa aataatattg aaacatgtag tcatagtaag
     2821 gagaagagtc ttttaaatgc tccagataaa tttgaagaac acttatcagg aggggaaaag
     2881 gttaagttaa aaatagcaga tgcgctgagt aataagaaaa atatcataat agctgatgag
     2941 ccaacttcaa acttagataa aaaaagcatt ggggttttgg aagatatgtt taaaaggcat
     3001 gagggagcat tattattaat atctcatgat agacgttttc tggatgagtt atgtacaact
     3061 atattagaac tagaagatgg aaaactaaaa gcttacaaag gcaattacac tgattattta
     3121 atgcaaaaag atgaagaagt aaaaagagct gattttgagt atcaagaata tgttaaagag
     3181 aaaaaaaggc ttgaaaaagc tcttttatat aaaaaagctt taagtgatgg cataagaaaa
     3241 acaccaaaaa gaatgggtaa ttcagaagct aggttgcaca aaatgggtgg tcagactaat
     3301 aagaaaaagt tggactcaaa tgtgaaggct ataaaaagca gaattgataa acttgaggta
     3361 aagaataaac ctaaagtttc taaagaaatg aacattaaga ttcaagatgg tatggagata
     3421 attagtaaaa atctagtaga ggtaaaagat atgactctaa agttagaaaa taagctcctg
     3481 ttagataatg tttcctttaa gataaaaaga ggtaaaaaga tagcattatt aggtgataat
     3541 gggtgtggaa aaagtactct gataaaagaa atattggctg ataaaaatga caatataaaa
     3601 ataaataaca aggtaaaagt aggttatttt gaccaaaatc aaagtttatt agatgaggaa
     3661 aagagtgttc tatacaatac taaagttaat agttcatttg atgaatcttt tataaggata
     3721 aacttaagcc tatttgggtt taaaggtgat gatgtctaca aaaaagtaaa agtacttagt
     3781 ggtggagaaa aggtaaaaat agcactatgt aaaataatat tggaagataa taatttttta
     3841 gtatttgatg aaccaacaaa ttacttagat ataaaatcta tggaagcact ggaaaaggca
     3901 ctgataaata ctgataaaac tatgcttata gtgtctcatg atagagtatt tgtatcacat
     3961 atttgtaact atattataga gataaaagat gctaaaataa gggagtttga ttgtaattat
     4021 gacgaatata caattagcag aaataaaaaa actcctagta gagaaaacca aatcaaaaaa
     4081 gaaaatcttc tagtattaga aaatagactt acaactgtta tatctatgtt atctatagaa
     4141 aaagataatc taaaaaaaga attgtatgaa tcagaatata atgaattact aaaacaaatc
     4201 aataaattaa aaaatagctt ttagtgttaa ctttttaatt acatctatat aagaagactg
     4261 aaaatatttg ttgggaatat aagtatagtt attaaaacag aaaagtattt tgttagaaaa
     4321 ttaaaagtaa gctaattaga aagtggcctt attattgtac tttataagaa ggccactatt
     4381 tttatttaga atgataatgt gtagttgtct aacatttatt ttgtatttag tgcagaattt
     4441 attcagattt aaccttaagc ttggctactg tttcttcttc aggagtaaca taaatagtac
     4501 tgttagtttc ataaataacc atacctggtt tagaaccact aggttttttc acattttttc
     4561 tcttagtata gtctacaggc acctgagaag aaagttttga cttactaaag aatgctgcta
     4621 gcatagctcc ttcaaataca gtattatcag ggacttcctt accagcacac tttattatta
     4681 catgagaacc aggaatattc tttgtatgca tccataagtc atcattatca gcaagtctta
     4741 gtgtaagata gtcattttgc ttattgtttt taccaaccaa tattttaaat ccatcagaag
     4801 aaacaaattc atgaggtttt gtagatggga tagtatcctt tttactgttt ttcttttgag
     4861 tcttgatgta tccaacctta gcaagttctt cttttatatc ttgtaattca gctaaatttt
     4921 cacagttttc aatacttaaa attatgtttt ctaagtaatc tatttcttcc ttatttaagg
     4981 aaatttgatg agatatctcg actttagcat gtttcatttt gttatacttt ttaaagtatt
     5041 tttgagcatt ttcagatggt gtcaagtttt tatttaattc tattgttaca tcattacaat
     5101 tttcatcata gaagttagca acttcaatac tttccatacc tttttctacc atataaatat
     5161 atgaagtaat caattctcct tttatcttat aaatgtctgc attttcggat tctgataatt
     5221 cttcttcttg ttttttaagt ttattataaa gtctatcaag cttaacagat atactcttct
     5281 ttaaatcaga tgaacgttga ttaattctat ctttaatatc cttagttcta tagaaatctt
     5341 ccagaatacg agacatacta tccttattta tatatgataa atcgctaaat aatgtaaggt
     5401 ttatacaact gaaatcaact accttatcca cctttttatc tataataatg caaggagagt
     5461 atttgttaga atttatatca ttgaataaat tacaaaatac tttgtgtaaa gaatcaaatt
     5521 gttcatcaga tatatccttt atgatagcat tttgatttac acctgctctg tagcaaattt
     5581 ctttagctat aatagggctt atacctagaa atttagaata tatagactta aagataggtc
     5641 cttccaattc ctctaggttt ttaataaaca aatccttaga tatatcatca agagggttga
     5701 gtttgttttg ttctggtggc aatgaataat catgaccagg taaaacttga cgaactctac
     5761 ttatgctaaa agggactctt tttatagaat ctataatttt attttcttca ccatgagtta
     5821 ttattatgtt gctatgtctt cccataattt caatcataat atttttaact gtcttttctt
     5881 ttaattcatc taaagattca acacttattt ttattattct ttcaaaacct atttgagaaa
     5941 tttctactat atttccacct tgtatgtatt ttctaagtaa catacaaaac ataggcgctt
     6001 ttagagggtt ttctttttga taattacttg taagataaac tctaggattt gaggcacttg
     6061 cacttaaaac caatctaaaa ttttctttat tatttcttat attaaaaatt acctcatcat
     6121 cttcaggttg atgtatctta tctatttttc cacctgtaag ttttgatgat aattcatcga
     6181 ctattgaatg tataactaat ccatctaatg ccatttatag cacccctttc tttgaccaaa
     6241 atgagtcagt aaattaatta tacacataat atacgctttg tgactagggg gaatttaaac
     6301 aatagagatt aatatttaaa ttgtataatt atttatattt attagagagg aaactttatt
     6361 tttaaaatta ataatttaaa gtagattaaa tataaagtaa atatagtata attattgtgt
     6421 agagtataag ctatgaaagt atatgaactt tacagaaaaa atcaatttta aggttctata
     6481 gtatataaat atgaaagaag gttgaaaaaa tgagatttat cgatttaaga agtgatactg
     6541 ttactatgcc tactgacgaa atgagagaag ctatggcaaa ggctcctgtt ggagattcaa
     6601 tctttagaga tgaccctact gtaaataaac ttgaagaatt agcagcagct aaagttggaa
     6661 aagaagatgc tattttcctt ccaagtggaa cttttgggaa tcaattagct ctatttactc
     6721 attgcctaag agggcaagaa ataattattg gtaaaggtta tcacattgtg actcatgagg
     6781 ttggggcacc tgctgtaata gctggagttc agcttagaac tatagatgaa gacgaaagtg
     6841 gagctctaaa tcctgaactt gttgaaaagg caattagaag agatgacata catgaaccac
     6901 agacagggct tatatgtgta gaaaatgcat attcaggtgg aacagttgtg agtcttgata
     6961 atttaagaga aattaaaaaa atagctgaga aacataactt accagtacat ttagatggag
     7021 caagattatt taacgcagca ttaactttgg gtgtagaagc taaagaaata gcaaagtgtt
     7081 gtgatagtgt tatgttctgt gtgtcaaaag gtttagctgc accaatgggt tctatacttg
     7141 ctggaaataa agaatttata gcaaaagcta gaagaaagca aaaagttatg ggtggaggaa
     7201 tgagacaagt aggtatagtt gcagcagctg gtataattgc cattgaaaaa atgactttaa
     7261 gattgaatga agaccatgaa aatgcaaaat atttagcttg tgaattaaat aaaattgatg
     7321 gcattgaagt aaacaatgtt aatccagata taagtatggt attttttaag atgagtgaag
     7381 atattataaa agaggatgtt ttaataaaag aattttttga aagaaatata aaaattaata
     7441 aaatggaaaa tggtgaatat agatttgtaa ctcatgtaga tattactaag gaagatttag
     7501 actatgtttt aaaaacactt aaagaattaa taggtgtatg ttaatagata tctactagaa
     7561 ggattatagc tagttaccag atgtttaatt aaaaaattca tagacagttt aaataaaaca
     7621 tacataaaat tcatatagaa atattaatat ataaacatag aaagaactag cttaaaaaca
     7681 taatataatt accctaattg taatggcaac tctccctcac atcctcaaat tatagttgtc
     7741 attacaaaac ttttcccata aattataagt ttatgggaat ttttttatgt tataatagtt
     7801 taagtgttaa tttacgaagg agcgatactt atgaaatttt ggaaactaca tggaatagga
     7861 aatgatttta tagcaataga tggaagattc gaccagataa atccatctga ttatactgct
     7921 ttagcaaaaa aagtatgcca tagaagattt tctgtaggag cggatggatt gttagttgtg
     7981 aaagattcaa aagttgcaga tgtagaaatg gtatattata attctgatgg ttcaagagca
     8041 gctatgtgtg gaaatggcct tagatgtttt tgtaaatttg tttatgataa ttgtatagtt
     8101 aaaaaagaaa gttttacagt agatacatta gatggtataa aagatataaa agtaaatctt
     8161 actaatagag aaataaactc tataagagtc aatatgggaa aaggaagttt tatagctaag
     8221 gacgttcctg tacttacaaa taaagaacgt tttgtgcaag aaagtataaa ggttttagat
     8281 agggagctta aagttacttc tatgttgatg ggggttcctc atacagtagt tatagtagat
     8341 gaattagatg tagatgatgt ttgtaaatat ggaaaagaga ttgaaaataa taaaatattt
     8401 cctgaaaaaa cgaatgtaaa ttttgtaaag gtagaagata agtataatat acatgtttat
     8461 acttgggaaa gaggttgtgg atatacttta ggttgtggta ctggtatgac tgcatcagca
     8521 atagtgtgca atttacttaa tatggtagaa agtagtgtaa atgtaacgtc tcaaggtgga
     8581 acagttaaaa tagatgtagg agatttctct tatatgacag gtccagcagt aaaaatatgt
     8641 gaaggtattt tggaggttta attaatggct ataagtatga ctggttttgg aagaggagaa
     8701 tacaaggatg ataattatta ctttttagta gagtgtaaaa cgataaatca taagtattca
     8761 gatataaaca tacgtcttcc aagaaaaatt tcatttttag aggataaagt tagaaattta
     8821 gtaaaaaatt atgtaaaaag aggaagagta gatttataca taaagtttga cttattaggc
     8881 aaagaagatg taaacttaaa ttttgatgag ggattagcat ctcagtatat agacatacta
     8941 aaagaaataa aaaataaatt tgatattata gatgatatta gcgttatgaa tgttgcaaag
     9001 tttcctgata ttgtgaaaat agaagaaaag gaagaagatg aagatttatt gtggtcaatg
     9061 ttgaatcaag cagttgaaga tgcattaatc aaattaagag aaatgagaag tgaagaaggt
     9121 aaaaaattag ctgaagatat tgccatgaga tgtgatttat taaaaaatca tatagaagag
     9181 attgaaaaat attcaagtag tgttgtagaa gattatagag aaaaattgaa tttgagaata
     9241 agtgaattac tggatgaccc aagtataata gatgagaata gattagcaca agaagttgca
     9301 atatatgcag ataaaagcag tataacagaa gaaatagtta gatttaaaag tcatataggg
     9361 caacttaaaa atactatatt taaagacgac tctataggta gaaaaataga tttcttaata
     9421 caagagatga atagagaaac taacactata ggttcgaagt catctgatat aaatataact
     9481 aatttagtag tagaagtaaa aagcgaattg gaaaaaataa gagaacaaat acaaaatata
     9541 gagtaagaat acaatattag aaggggaatg cttaaatgtt tgtgaaaaaa ggattattgc
     9601 tagttgtatc aggaccatca ggtgcaggaa aaggtactat ttgcaaagaa ctacttaaag
     9661 aaaatgatac aataaaactt tctgtatcag ctactacaag aaaacccaga actggagaag
     9721 ttgatggagt aaattatttc ttcataagta aggaaaagtt tgaagagatg atagaaaaag
     9781 gtgagttttt ggagtatgct caaatttatg ataattttta tggaactcca aaagcggcaa
     9841 taatggaatg tttagaaaaa ggacaagatg tattactgga gattgaaatg caaggagcaa
     9901 aacaaataaa agaagtttgc ccagagggag tatttatatt tgtactacca ccatcactta
     9961 aagaattaaa aaatagaatt gttggaagag gtacagaaac agaagctgaa atagagaaga
    10021 gatttagctg tgcatatgaa gaaattaaaa tgattaagga ttatgattat tttatattta
    10081 atgaagatgt aaaaacatct gctaaagaaa tagaaggtat aatatcatct gaaaagaata
    10141 aagttagcag atataaaaat attataatag agaaatttaa ggaggaatta taattatgtt
    10201 aaaaccatct atcaatgagg tactagaaaa aatagacaac agatactatt tagtagggac
    10261 tgtttcaaag agagcaagaa aattaattga tggagaagaa ccatatgttt caaataaaac
    10321 taaggaaaag cctgtatgtg tagcaactaa agaagttgca agtggaaaaa taacatatag
    10381 attattgaca gaagaggaaa tagagataga agaagctaga catcatgctg agcaacacca
    10441 acaaatttca gaagaggagt aaggtcatgc taaaagataa aactgttgta attggcataa
    10501 gtggcggtat agctgcatat aaggcttgtg atgttgtaag taagttaaaa aaactaaatg
    10561 caaatatcca tgttataatg acaaaatcag caacagaatt tgttaaacca cttacatttc
    10621 aatcgctgag tcaaaattat gtagtagaag atatgtttga agaaccaaaa acttgggaag
    10681 ttgaacatat atcattagca aaaaaagcag atttattctt aatagtgcca gcaagtgcaa
    10741 atgttatagg caagattgca aatggtatag ctgacgatat gttgacaact actgttatgg
    10801 caacaaaagc acaggttctt atagcaccag ctatgaatac taatatgtat gaaaatccaa
    10861 ttgttcaaaa aaatatacaa actttaaaga gctttggata taaatttatt gaaccggaaa
    10921 gtggaaggct tgcatgtgga gatattggaa caggtaaact agctaatcca gatataatag
    10981 tagaggaagt tttgactaat cttactaaag agcaagattt aaaaggtcaa agtatgatag
    11041 ttacagctgg tccaactgta gagagtatag acccagtaag atatttaact aataggtctt
    11101 ctggtaaaat gggatattct atagcaagag aagctatagc tagaggtgct gatgtaacat
    11161 taatatcagg tcctacaaat ctttctattc cacaaaatct taaaaaattt gttcaaatag
    11221 agtcagcaca agatttgtat gaagcagtag tatcaaattt aagtgaaaat aaaatagtaa
    11281 ttcaaagtgc agcagttgca gattataaac caaaaactta ttctgacaaa aaaattaaaa
    11341 agaagaatga tgatttatca atagaactaa gtagaaatta tgatattgct tatgaaatag
    11401 gaaaaataaa agaagataag atactaattg gatttgcagc tgaaactaat gaccttatag
    11461 aacatgccaa aggaaaaata aaaaagaaaa atttagattt tattgtggca aatgatttaa
    11521 ctaaagaagg tgctggtttt agagctgata ctaatattgt aaaaataata gatgtagagg
    11581 gaaatattac tgagtatcct aagatgaaaa aagaagaagt agccaatgtt attttagata
    11641 aagttaaagc gttgcttgag aaataagacc ttttttaaat atgatatttg tgtattttat
    11701 cacagctaaa gtggctgaaa gctgcaattt aattttagcg agtaaaaact cgcaaatgtc
    11761 agttaaattg tagttttcag catttatttt gtcataaagt ataaaatata attaatagtt
    11821 aattattaaa ataaagttaa tattaataac ttgaaattgc ttataaattc tgagatttta
    11881 atatcttgta aagtatatta aaatatgaac aatatattaa atttaagtag aggttttata
    11941 atgaaaaaat atgcaaaggt aatagttaga agtaatacta tatatacaga taatttattt
    12001 acatatcaaa ttcctgtatt tttatctgat gtaataaaaa ttgggcacag aatattagta
    12061 ccatttggaa aaggtaataa accaacagaa gcctttgtat ttcaatttac agatagctta
    12121 gatgaaaaaa taaaaataaa agaaattatt gatatattag atgaaaatcc gatatttaga
    12181 aaagaagatt tagagttggt ttattggatg aaaaatagat atttgtgtac ttatatagaa
    12241 tgtataaatt tgatatatcc aaagggatac aaattaaata attataaagt agtcctatta
    12301 ggtgaaagtt taagtggatt aaatgatatt gaattgaagg aaaaaatatc aactttaagt
    12361 gataaaaata gagaaatagt agagaaagtg attgatagta aaggtaaaat taaggttgat
    12421 aagttaaaat atataccaaa cttaaatagt tctttgtaca caatgaataa gaatggaatt
    12481 ataaaattat gttgggaata taaaaatcac aaaaatgaaa agaaggtatg ttatatatct
    12541 ttgagtttag aaagtgataa gattgacgat tatatagagc aaaataaaat aaatgtggga
    12601 agtaaacaaa aagagatatt aagttttctt aaaaataatg aaaatgtaga gataaatgat
    12661 ttattggatt tattaaatgc ttcaaaacaa agtataaatt ctctaagtaa gaaaaagcta
    12721 ataaccctag aatttaagga ttattataga gagccaaaaa gtatttacaa atccgtacta
    12781 aaaagtataa aattaaacaa tgagcaacaa gaagcagttg atgagataaa gtcgaatatg
    12841 tttgtagatg ataaaaaacc atacttaatt catggagtaa ctggaagtgg aaaaactgaa
    12901 gtttacatgg aaataataga atttgctcta aatcaaggct tagacagtat attcttagta
    12961 cctgagatag cattaactcc tcaaacaata gataggctaa agagtaggtt tggagatttg
    13021 gttggagtgt tccacagtaa actatctgaa ggagaaaaac atgatgttta caaagctgtc
    13081 aaagcaggta aagtaagagt gttaattgga gctaggtcag ctttatttgc accttttaat
    13141 tccttaggct taataataat tgatgaatgt catgagtcat catataagtc tgagaagaat
    13201 ccaaaattta atgctataga agttgctaga tttatggcat taaaaaataa tataactctt
    13261 attttaggtt cagcaacgcc ctcaattgaa gagtattata gggcaaaatc tggagaatac
    13321 aaacttataa atataaaaag tagagcaaat gataagcctc ttccaaacat tgaagtggta
    13381 gacatgaaag atgagttaga taaaggaaat agaagtatat ttagtttgaa attacaaaaa
    13441 gaaatacgtt atgcgattga agaaaataat caagtaatac tatttttaaa cagaagaggt
    13501 tatgcaaatt ttgtatcttg tagaaagtgt ggatatgtgt ttcaatgtga aaattgtgat
    13561 atatctttaa cttatcataa gaaaagcaat actggaagat gccactattg tggatatgag
    13621 aaagaaattc caaaagagtg tcctgaatgt aaaagtactt atgttaagcc atttggagtt
    13681 ggaactcaaa aaattgaaga ggaattaaaa tatatttttc cagatataaa aactttgaga
    13741 atggataaag atacaacttc aaaaaaaggt gctttagatg agatactaaa taaatttaaa
    13801 gataaagaag cagatgtact tattggaaca cagatgttaa gtaaagggct ggattttgag
    13861 aatgtaactt tagtaggtat tttgtctgca gacatgatat taaattttcc agactttaaa
    13921 agttttgaaa ctacatttca attgataact caagtctctg gaagagcagg gagagctgat
    13981 aaagagggaa aagtagtact tcagacttat gacactgaac actatgctat taagcatgct
    14041 attgaatatg attatgaggg tttttatgaa gatgagataa aaataagaaa agcatttgga
    14101 tattctcctt ttaataatat gttaagtgta gttgttagtg gtgaagatga aagactagtg
    14161 ataaaaaata taaaaaatat gcatgcatca ttgatttatt tattagaaaa aaggggtata
    14221 aatgatttag gatttatatt aggtccaaat ccatgttcaa tatcaaaaat aaatcaaaat
    14281 tatagatggc aaatactctt taaagatgaa aatattgaaa ttaatctctt aaagggtata
    14341 ataaaatata tatgtataac aaaacgagat ttgatatttg ataagaatat caatgtatct
    14401 atagatatta atccaaacag tgtattataa atttaggagg aattataatg gctttaagac
    14461 aaatagttca aataggagaa ccagtattaa gaaaaaaatc aaaaaaggtt gaaaaaattg
    14521 atgaaaagat aatacaatta ttagatgata tggcagaaac tatgtatgat gcagatggtg
    14581 ttggactggc tgcacctcaa gttggaatat taaaaagagt agtagtaata gatattggag
    14641 aagagcttat agaacttata aatcctgaaa taatagaaac atctggggag caaatagatg
    14701 aggaaggatg tttaagtgtt gttggagaag cgggaaatgt tagaagacca aattatgtaa
    14761 aggtcagagc tttaaataga aatggtgaaa caatagagtt agagggagaa gaacttttag
    14821 ctagagcatt ttgtcatgag atagaccact tagatggaat tttgtttgtt gacaaaatag
    14881 aaaagtagta ggagttgatt tgatgaaaat agtatttatg ggaactcctg atatagcagt
    14941 tccatgttta caaaaaataa tagatgagaa gtatgaaata ttgggtgttg taactcaacc
    15001 agataaacca aaaggaagag gtaaaaaact aggtatgagt ccagtaaaag aactggcaat
    15061 tgaaaataat atacctgtat atcaaccagt aaaggctaga gataaagaat ttatagataa
    15121 aattaaatct ttaaatccag atgtaatagt agttgtagct tttggacaga tacttccaaa
    15181 agaaatatta gagattccta aacttggatg tataaatgtt catgtttctt tacttccaaa
    15241 atatagaggt gcggcaccta taaattgggt aataataaat ggtgaagaaa agactggtgt
    15301 tacaactatg tatatggatg aagggctaga tactggagat atgatactaa agacagaagt
    15361 aaatcttgat gaaaatataa cagcaggaga acttcatgat aaaatgatga atataggcgc
    15421 agaaacttta aaagaaacat taagattaat tgaagaagga aatgctccaa gagaagtaca
    15481 aaatcatgaa gaattttcct atgcgccaat aatgaataaa tcattaggta acatagattt
    15541 ttcaaaaagt gccagagaaa tacacaattt agttagagga gtaaatccat ggccaagtgc
    15601 ctatactacc tataatgatg tcataatgaa agtttggaaa actaaagtgt tagatgaaaa
    15661 aagcacaaaa gatgtaggta caataattga tgtaagcaaa gatgggataa aagtaagtac
    15721 tatagataat gtacttttaa ttgaagaaat tcagatgccg aataaaaaga gaatgttggt
    15781 tggtgaatat ataaaaggga atactataga aactggttta gtattgggtt aggtgaaaag
    15841 tatgacagat ataaagaagt atttaacaag tgttgggatt ttgattttaa tattaggaat
    15901 tttgatagga gttgttaatt tttttataat aagtttaact caaatattct ccttgatgat
    15961 aaatattata ataatatcac taatacttat aatactatgt tcaacagcag ttacttatag
    16021 agtaattaaa ggaaagtatg ttaactcaaa tataatgaaa ataaatttta atattgtcag
    16081 tggattattt cctattatat ctttcattgc atcatcattt ggtatgtcaa aaagtgatat
    16141 tagaagaata tatataaagt taaataatga atatatatac agtaataaat ataattttaa
    16201 tccagaggat ataataattt taatacctca ttgtattcaa gagaatagtt gtaaattaaa
    16261 agttactaat gatatagata attgtaaaga atgtggaaga tgcaacatag gagaattaat
    16321 taagctaaag gaaaaaacta atgtaaaaat atttgtagct actggaggaa ccctagctag
    16381 aaaaattata atggataata aacctaaagc tgttgtagct gttgcatgtg aaagagattt
    16441 gacatctgga atacaagata ttaagaaaat tcctgtttta ggtgtattta acaaaagacc
    16501 taatggacct tgtgtaaata ctaatgtaga cattatggat attgaaaaag ctataggctt
    16561 tttaacagga aaaaatatat tagtttgtaa ttaaactgtt tttaagtgaa gtgaatatta
    16621 agaggatgta taataaaaat atattttagg agagtgttat ttatgtaccc tgtatatggc
    16681 ggtttttggg gatttgaccc aacaatggtt gtattaattc cagctatatt acttactata
    16741 tatgcacaat ttaaagtgag ttcaacaaca aataagtatt taagagtaaa tacacgtcga
    16801 ggatatactg gagagcaaac agcaagaaga gtacttgatt caaatggttt gtatgatgta
    16861 aagatagaaa tggttagagg gcatctaagt gaccattatg acccaagaag aaaggctgta
    16921 aggttgtcag aagatgtata ttatggaaca tctataactt ctgttgcagt agctgcacat
    16981 gagtgtggtc atgccataca acatgctaaa ggttatgcac ctcttcaaat aagaagtagt
    17041 ttagtaccag tagtgaattt tgcttcaagt atatcttggt ttttaatatt tttaggattt
    17101 attatggctg gtccattttt aaaaattggt atactattgt tttcggcttc agtgttattc
    17161 caaataataa cattgcctgt agaatttaat gcttcaagta gagcaatcgt tcaacttggg
    17221 aatttaggga ttatagatga aagtgagtca agacaaagta gaagagtact ttcagcagca
    17281 gctttaacat atgttgcagc agcattggtt tcaatacttc aattacttag attgttacta
    17341 atagctcaaa gaagaaatga ctaaaagtaa agtgggttgt ttagaaacaa ccctttttta
    17401 tgtttaataa aagggagaaa tttatatgga tgcaagagaa gttggattta aagtattatg
    17461 tgatatagaa aaaaatgaca attattcaaa tattgcaata aataagcatt ttaaaaattt
    17521 agaaataagt gatatggata gaggacttgc aacagagtta atttatggag taatagaaaa
    17581 taaatattat ttagattata taatcaataa actttcaaaa ataaaggtaa aaaaaatgtc
    17641 aacttatgtt aaaatctttt taagaatggg aacatatcag attttatttt tagatagtat
    17701 atcagattat gcagcagtta atgaaactgt aaaattatct aagaaatatg ataaaaaatc
    17761 ttctggtttc ataaatgcta ttcttagaaa tgaaataaga gctaaagaga ctataatgga
    17821 cataactgag gaagatagtg taaaatactt atctataaag tattcctata actcttggat
    17881 tataaaaaac tggatagata aatttggaca agaatttgct gaagatttgt tggaagcaaa
    17941 taatgaaaag cctagtatct atataagaac gaatacactt aaaataagta gagaagaact
    18001 tattgaaaaa ttaagtgaaa tgggtattgt atgtttaaaa gttcctatgg tagaagaagc
    18061 tattaaagtc gaaaaaatga aaaatataga aaaaaatgaa ttatttaagg ctggtttatt
    18121 tacaattcaa gatataagtt ctatgatagt agggaaagta attaatccaa aagaaggttc
    18181 atttgttttg gatgtatgta gtgctcctgg agggaaatct actcacttag ctactttaat
    18241 gaacaatact ggtcaagtag tagctagaga tatttttgag cataaactaa agcttataaa
    18301 ctctactgta aatagattgg ggcttaaaaa tgtgtttgta gaaagatttg atgcttctga
    18361 gattgatgaa aatagtataa gtaaatttga ttatgtttta gcagatgttc cttgctctgg
    18421 ttttggtata ataagacgta aaccagaaat taagtataag aaagaagaag agttaaaaga
    18481 catcacttct atacaaaaga aaattttaga aaatgcatct aaatatgtca aagttggtgg
    18541 aactttagta tatagtacat gtacaattca agatgtggag aatataaata tagttacttc
    18601 ttttgttgaa gaaaatcaca actttgaatt tgttccaatt gatacagtaa atgtagactt
    18661 agacaatcaa gataaaggat atttaaagat atatcctaat attcatggta tagatggttt
    18721 tttcatagca aaattaaaga gaataaggta gtgagagaat ggaaaagaaa aagatagtat
    18781 taaaaaattt tactgaagat gaacttaaag agtttatgaa aactatagat gaaaagccct
    18841 ttagaggaag tcaaatattc tcttggatat ataagggtgc aaaaactttt gatgatatga
    18901 acaatatacc aaaaagtttg agaaataaat tagaagaggt ttcatgtata gggcatatag
    18961 atatagaatt aaagttagaa tctaaggtag ataatacaaa aaaatatttg tttttattag
    19021 atgatggaaa tataatagaa actgtgatga tggattatga tagtagagtt actgtctgtg
    19081 tttctaacca agtaggttgt aggatgggat gtaatttttg tgcatctaca atggatggat
    19141 taattagaaa cttagagcca tgggaaattt tagaccaagt tattaaaatt caagaagata
    19201 ctggtaagag ggtatcaaat cttgtattga tggggagtgg tgagccactt gataactttg
    19261 aaaatacaaa acaattttta aaaataatta acgaaaaaaa tggtcttaat atagggtata
    19321 gacatataac tctttcaact tgtggaatag tacctaaaat gtatgagttg gcagacttag
    19381 aaatagctat aaatttagct ttatcacttc attcaccata tgatgaagaa cgaagaaaaa
    19441 ttatgcctgt agcaaatgct tattctattg aagaaatatt gaatgcatgt agatattata
    19501 taaaaaagac aaatagaagg gttacatttg aatattctct tataaaagga gtaaatgact
    19561 cagaaaaaga agcaaaggca ttagctaagc tcctaaaagg tatgctatgt catgtaaatt
    19621 taattccaat aaataaagtt gaagaaagag aatacgaaaa accagataaa gcatttattt
    19681 ataaatttag ggatagtttg gagaagaaca atatacctgc cacagttagg atgtctatgg
    19741 gttctgatat tagtggagct tgtgggcaat tgagaagaaa atacaagtaa tttgtaagga
    19801 ggaagtcgta tggtttatag ttgtgcctcc catataggaa aaataagaaa aaacaatgaa
    19861 gactattgtg agggagaagt tatagataca gaacatggtc caataggaat atttgccata
    19921 gctgatggaa tgggtggaca taaaaaaggt gaagtcgcca gtaaattagc agtagaaaat
    19981 ataattgatt ttctaaaaga aaacttatta caacatgata atgtaaaaat agactatata
    20041 gatgatatat taaaacaggc ttataataat gtaaactcaa ttgtgcacaa aaaatctatg
    20101 gaagatatag aatttgaagg aatgggaact acattagtta cggctatagt gtataataat
    20161 gtcttatatg tggcaaatgt tggagatagc agatgttatt tgttaacaga tgaaaaattt
    20221 gataaaataa ctatagacca ttccgtggtt gaagaactta tgatggccca tgtcattaca
    20281 gaagaagagg caagaagaca tccacagagg aatagaataa ctagagcaat tggaacagat
    20341 gatatggttg ttgtagatat ttttaagaaa gaaatcaaaa aaagtgatat aatacttctt
    20401 gcgacagatg gattaactgg gtttattgat gatgaagata taagagattt aatattagat
    20461 tatgaatatg agagaactag taatataagt gaagaattga taagtatggc caatgatgtt
    20521 tctggtaaag ataatgtttc ggttatagtg ataaaagtat aattaggacg gtgatattgt
    20581 gggagataca attttaggaa atcgatatga aatcatcagg aagattggtg atggaggaat
    20641 ggcctttgta tatgaggcta aagacagatt attaaacagg actgttgcac ttaaagtact
    20701 tagacctgaa tttgtagatg atgatgaatt tctaacaaag tttaaaagag aggcagaagc
    20761 agtagcaagt ctttcacatc caaatatagt aaatgtttat gatgttggtg aagatggaaa
    20821 agttcactac attgtaatgg agtttgtaga tgggaagaat ctaaaagaga ttatacaaga
    20881 tgaaggtata ttagatgaat atactgcatt agatataaca aaacaaatag ctatggcact
    20941 tagtgcagcg cacaaaaagg ggattataca tagagatata aaacctcata atattctcat
    21001 atcaaatgaa ggaagagtag taaaagtggc tgattttggt atagccaaag ctgtttcaaa
    21061 ttcaacaatg actaatattg gtagtataat aggttcagtg cattattttt caccagagca
    21121 agctaaaggt aaatttgtaa ctaataatgc tgacctgtat tctctaggta tagttttata
    21181 tgaaatgttg ataggaaaag ttccatttag gggagatagt ccaatttcta tagcacttca
    21241 acacattaat gatgatatag actttacatc agaagaaaaa gttaggattc ctcaaagtgt
    21301 aagaactaca ataaaaaagc ttacagaaaa atctagtgca gatagatatc aaactgctga
    21361 agaattgata gaagatatag agtatataga aaaaaatatt gatttagatt ttataaaaga
    21421 atatgatgat tttgcaacta agaaaattga tgaaaaagaa attaataaag ttgtaaatcc
    21481 aacacttgca aaaccagctc ctgaaaaagt ggttaagcct gttgaagtag ctgatttgga
    21541 tgatgatgaa gattactatg atgattttta tgaagaagat gatgatgaag atgaagaaga
    21601 ggaagagata atgagagcta aaaagaatca gagacctaag agtactccaa gtaagagaac
    21661 taagaaaaag aagaaaaaac aggaaagccc aaaatctaga agaagattaa aagtaattgc
    21721 ggctgttttg atactgatat tgtgtgctca agtattctta gcatataagt ttttatttgc
    21781 tggagggttt ggcaataaat ctttgactgt tcctaatctt gtaaatatga cattagaaga
    21841 agctcaaagt gctgttgaaa aagaaggatt atacctgagt gtaaaaagtg aagagtataa
    21901 tagtgaagtt gatgaaaatt gtataatatc acagacacct gaaggtggaa gtacaaatgt
    21961 caaaaagggt gatactataa atgttgtcgt aagtaaaggt tcaagtcagg catctgttcc
    22021 taatgtagtg ggacttactt tgagtaatgc aaaacaactt attgaagaaa ataacttaaa
    22081 agtgggaact gttaagtatg aatacagctc aatatataaa gaaggtacag ttttaagtca
    22141 gagtccgggt gctggctcat caagagctca ggaaggtgat gaggtaaacc tatatgtaag
    22201 caaaggttca gaaaaatcaa atacacagac tccaacagta ccagataaaa aaccaacaac
    22261 accagagaat aatacaccaa cagaacctgg ttcaaactca ggaaacagtg gtggaaattc
    22321 aggaggctca aattcaggaa atagtggtgg aaactcaggc aataatggaa gtaactcagg
    22381 aggttcaaac tcaggaaata gtggtggaaa ctcaggaggc tcaaactcag gagataatgg
    22441 aggaagttca ggaggctcaa actcaggaga caatggagga agttcaggag gttcaaactc
    22501 aggagacaat ggagatagtt caggaggctc aaactcagga gataatggag gaacttcatc
    22561 aggaactggc gcaaacattg gtaaaacaga ataaatatta ataaaataag agctgtcctt
    22621 ttatagaaaa tattttcttt aaagggcagt ttgttttgta taaaatactg aattggttat
    22681 aatataagga gaaaagtatt tatgaagaag aaaacagttt taattacagg aggagctaga
    22741 gggattggta aagctatgtc taaagctttt gcaaaagaag gttacaatgt attagtaaat
    22801 tttaataagt cagaaaatga agctaaagag ttgtacacta tattaaatga aaaaaatttt
    22861 tctgtaaaac tttttaaggc tgatatttca aatagagaag atgtagagga tatggtagat
    22921 tattgtataa aagaatttgg aggattggat gtacttgtaa acaatgctgg tgtaagtcaa
    22981 gataagttgt ttacagatat aactgatgaa gattgggata atatgatgaa tattaattta
    23041 aaaggaagct tttattgttc acaagttgct ttaaaataca tgatatcaga aaaaaaagga
    23101 aatataatta atatatcttc aatctggggt atatcaggtg cgtcttgtga agtgcattat
    23161 tctataacaa aagcaggcat aataggtatg acaaaggcat tagctaaaga agtaggacca
    23221 tcaaatatta gagtaaatag catagctcca ggtgtcatta atactgacat gttatctgga
    23281 tataatgagg aagatataga cgctcttgtg gaagaaaccc ctctaatgag gctaggaact
    23341 ccagaagata tagcaaattg tgctattttc ttggcatcag acaaatcaaa ttttatcact
    23401 ggtcaggtaa ttagtccaaa cggaggattt gttatttaaa aaaaatgtgg tattatataa
    23461 aaataggaaa gaataacacg atatactact cataaacagt atgtgtttat ataaatttta
    23521 taaagggagg catttgatgc tagaaggaaa aattataaag gggattagtg gattttatta
    23581 tgtagatact tataatggta tttatgaatg taaggctaga gggatattta gaaagcagaa
    23641 aataacacct ttggttggtg atagagtcaa aataagcatt gttgatgaaa atgaaaaaaa
    23701 gggtatctta gaagaaatcg acagtagaga tacagaactt ataagacctc ctattgcaaa
    23761 tgtagataaa gcattaatag tattcgcaat aaaaaatcct aaaccaaact tatctctttt
    23821 agatagattt atagttttgg cagaaaaaga aaatttagaa actgtaataa tccttacaaa
    23881 agcagattta gatgataatg atacattaga aactgttaaa aatatatatg aacttagtgg
    23941 ctataaagtc attcctgtaa gcaatattac taagttaaat atagataaag taaaagaaga
    24001 attaaaagaa aatgtagtag tgtttgcagg accatctgga gttggaaaat caagcttatt
    24061 aaatgaaata gatgaaaatt ttaaacttca aacaggagtt gtaagtgata aaataaaaag
    24121 gggaaaacat actactcgtc acgctgaact tttaaaatta gaatttggtg gaatggttgc
    24181 agatacacca ggttttagtt cccttgccct tgaagatata gaggaagttg aacttaaaga
    24241 ttattttata gaatttgata aatttaatga ttgtaagttt ggttcaaagt gtattcatga
    24301 aaatgaacct aattgtgcta taaaagaagc tgttacaaat ggagaaatat ctaaagaaag
    24361 atatgatagt tatatacagc tgctacatga aataagacaa aataatagta ggaggtatta
    24421 gaaacatgat taaattagca ccatcaatat tatcagcaga ttttgcaaaa ttattagaag
    24481 atgtaagaaa agtagaaagt gcaggatgtg aatatcttca tatcgatgta atggatggac
    24541 actttgtacc aaacattaca ttaggaccac ttgtagtcaa aagtttaaag aaagaaaata
    24601 taaatatggt atttgatgct catcttatga tagaaaatcc agaccagtat attgaagaat
    24661 ttgtaaaagc aggatgtgat ataataactg ttcaccagga ggcttgtgtt catctacata
    24721 gaactataca aaatataaaa tcacatggaa taaaagctgg tgtagtactt aatccagcta
    24781 caccagtaga tacgattaaa catgtactac ctgatttaga tatggtatta ttaatgtctg
    24841 ttaaccctgg atttggagga caatcattta ttccttgtgt tttagataag attaaagagt
    24901 taaaagctat tatagatagt caaggattaa acattgatat agaagttgat ggtggtataa
    24961 gccctaaaaa cgtagccgaa gttgtacaag caggagctaa tgtaatagta gcaggttctg
    25021 caatatttgg aagtgatgac attcaagaaa cagttaattt atttagaaag aatgcttctc
    25081 ttgaagaatt agtttaaatt taataatgaa aatctgtatt gttttaaatg gcgaaattga
    25141 agattacgaa gttacaagag atattataat taaggaatgt tatgactgca taatatgtgc
    25201 agatggaggg gcaaatcaca cttataaaat ggaaataatg ccagattata tattaggaga
    25261 tttagattca gtagaagaag aaaagataaa cttctataaa aataaaggtg ttaaatttga
    25321 aaaatttcca tctaaaaaag atgagactga tacagagctt tgtctttttt tagctaagac
    25381 acttaaagca aatcatattg atttttttgg tgctttaggg ggaaggattg accacactct
    25441 tgcaaatata aagctattat attatttaaa agaagatggt atatattcta ggatactttc
    25501 tgataaagaa gagatgtata tagttgaaaa tgaggaaatt tcattgtatg gaaacccagg
    25561 ggacactatt tctgtaatag ctataaatgg tgatgctaag ggtgtaacac ttactggctt
    25621 agaataccct ctcgataatt attatatgaa atactctgta cctattggaa tatcaaatgt
    25681 tatgttgagt aattcttgta agattaaagt agagcaaggc tgtgtacttg tagttagaaa
    25741 tttataaaat ttgattttga caattggttt tatattgtag agcagataca atcaagtaaa
    25801 atatatttat gattgtatct gctttttagt atataattat aaatatataa taaactttga
    25861 cataaggggg attttttgtg gtagatagta ttatttttga tttagatgga acattgtggg
    25921 attcaactga agaagtatgc aaagtctggc aggatgtttt aagtgagcat aaggaaatag
    25981 aattaagtgt cacaaaagat ttatttagaa gtcttatggg gctttctttt gaggaaatag
    26041 caaatcgttt atttccaaat ttaagtgaag aagaaagaat gaatttattg aatgaatgtt
    26101 ctttgaaaga atgtgactat ttatctgaaa atggagctaa attatttgat gatatagaag
    26161 atactcttaa attactatct aaaaaatata aattatttat agtaagtaat tgtcaagctg
    26221 gatatataga atctttttta aaagcacata aactagaaca gtattttata gattttgaat
    26281 gtcctggtaa tactggtttg cataaaggag aaaataataa acttataatt gaaagaaata
    26341 aattaataaa tccaatctat gttggtgata cgcaaggtga tgcaaatagt gctaagtttg
    26401 taggtatacc ttttatatat gccaaatatg ggtttgggaa tgtagatgta tatgattatt
    26461 ttatagattc atttaaagat ttgttagaac atgatatatt aaaataagat gagtttaaaa
    26521 attatattaa aataataaaa aagttatggg gggatttaaa aatggaatta caagatacta
    26581 tttttaaaag acaaagtgta agaaagttta aaaatcaaga tgtatcagat gaagatattt
    26641 taaaaatgat aaaagcagca ggtgcagcac catcaggcaa gaatattcaa aactggcatt
    26701 ttgttgttat aaaacgccgt gatttaatgg aaaagatagc agatgtgata actaaaaagc
    26761 aacaagaaat acttgtagaa atggataaag tatcagtgga taaggctaat agatttagaa
    26821 aatttgttaa aaattttaca ctattttact taaaagctcc agttttagta ttagttttta
    26881 caaaggtata taatccatca ggatattatg aattagagct tatagatgca cctaaggaga
    26941 ctatagataa attatttata agaaatccag gtatgcagag tctaggtgct gcaattgaaa
    27001 actttacact atcagcaatt gaacttggat atggttcttg ttggttgaca agtcaaaact
    27061 atgcagcaga tgagatagaa gctgttttag aagcagaaac tggttttgaa aagggagagt
    27121 attttttagg tgcaatgtta gctcttggag tgccagagga caatttgaag agtccatcta
    27181 aaaaaccagt agaagaaata tgtacattta taaaataaaa gtcaaggaca ttttaaaatc
    27241 gaagtaaatt aaaaacaaaa taaattaaaa ataaatattt aaaaa
//
LOCUS       DAMJYR010000042        26895 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.042, whole genome shotgun sequence.
ACCESSION   DAMJYR010000042 DAMJYR010000000
VERSION     DAMJYR010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 26895)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26895)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26895
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.042"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            244..3147
                     /locus_tag="PC471_001786"
     CDS             244..3147
                     /locus_tag="PC471_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDF2788917.1"
                     /translation="MELRDKLVNIINTENKKDPLTDVQIAKFLSTTRENITNLRKELN
                     ISNSRQRRYPYLKSAISAILQKNKNISISEITRELMTEGFNISRRVVEELLPKESLEM
                     DVVEEDEEESNDPFETLIGNKGSLRNAVEQAKSAILYPPKGLPTLIIGESGVGKSLFS
                     RHMYEFAKQKKIVKESSNFVVFNCADYSDNPQLLLSLLFGYKKGAFTGAEYDTPGLVE
                     EADEGVLFLDEIHRLPPKGQEILFSILDRGKFRRLGETNAERKVSIMLIGATTENVET
                     NLLLTFRRRIPMLITLPPLHDRLLKEKIDLIYNIFQQECNRINAKIFVDKNVIEILAL
                     KKFSGNIGQLQNMIQVLCARAFMKFINSKDEDSESIVVVDINEVLKLKDSFKDAAFQE
                     IEYTEIRKYLKNAIFIPFNLEESSVKGSLLSDEYILPEDIYKTIEKKYYDLKSLDIED
                     IDIENILWTFILNRFADLKFNLDSNEEIFSMSDLNSFVSDSLVKLVKELMNDIVEKNM
                     NLEVNKNMFKYLAIHLEEAIKRIKLNQKIINVNLEKIKIDFSKEYEISKSFAIKIEES
                     IGIKISDDEIGFITLYIKSALKNEVKKDKVGLIIISHGRIATETVNVVKELLGVKFPV
                     AIDMPLDEKPINIYNKAVELSKIIDQGKGILFLVDIGSLTNIGQIVNKRTNISTKTID
                     RVDLLMALEATRKVSMGEEELDEIFFSLLKDRMGYNYELEKHIDKSNAIITLCLTGEG
                     TAKYISKTLEEKYDNTKCYQMSALDESLFCKIENLKETNNILAIVGTINPKIPGINFI
                     PYNKELFKNLDIYLSKSKQQDDRPIKSYERMLDEDLVIFEPDIYFKKDLLEYVCSILI
                     NKEYVEKDYLDSVLHREEMLPTYSKGAIGVPHGDSSTVNSTRFVFVKLKNPIDWGVGN
                     VNFIFMPVFQANDKEIVKNVLEILKDAEFMNNANKCFDKDAFRRIIFDKFKHL"
     gene            3180..3626
                     /locus_tag="PC471_001787"
     CDS             3180..3626
                     /locus_tag="PC471_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDF2788918.1"
                     /translation="MNVDLISLGLESNDYESVIEELGSIMCKKEYVKETYINAVLERE
                     RTLPTGLDIGDMCVAIPHTDSKHVNESNVAVGILKNPVKFNSMIDPKDRLDVELVFLL
                     AVKNPDSQVKLLKDLMSVFQNIKLLKNIKNASTKEEVAKLLDFIEI"
     gene            3749..4051
                     /locus_tag="PC471_001788"
     CDS             3749..4051
                     /locus_tag="PC471_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDF2788919.1"
                     /translation="MKQFNVLSVCGSGTVTSSMVAGKLKEVLGNKGISITTTEARPTE
                     ALNLAQSGRFDFITFTSPLQTGDYGIPTINAFACLTGIGEEAFFEEVLTVIEGIDK"
     gene            4232..5638
                     /locus_tag="PC471_001789"
     CDS             4232..5638
                     /locus_tag="PC471_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HDF2788920.1"
                     /translation="MLEFMKSIIDTFGSAIIVPIIIFIIAKIFKVTTKKSFLSAVYAG
                     VALQGFTLILNSFTPVITPVINRMVESTGVNLPVFDVGWQATSLVAFSTSAGMIYLGL
                     GILLQTILFLIKWTDVFQPSDLWNNYSYMVWGAMVIGVTGNFPLGIACMVLLNLYSLL
                     ISELVAKRWSSYYRYPNCTIIAMHNVEASVFAVFADPIYNKLGLNKIKLNPKELEKKL
                     GFLGEPITLGLFLGMFIGILGNMTRINTMEAWGEIMKVGISTSAVMAIFPKVASMFAQ
                     AFAPITEAARKIMQKAGNREWYIAVNDAVGYGEPATLISGLILIPIMLVIAMVLPGNK
                     VLPVVDLLAIPYMVQGLVAIHNGNIPKVLVSGIIWFGLGLYVCTSTAPLFTDMATNIG
                     VAIPAGAMLITSFNILGKPLMGLVFFAFLSANPIYIGLSVVIYFVLWALFRKNKTSIL
                     DYLEKQALKNVEEEPVAV"
     gene            5982..7040
                     /locus_tag="PC471_001790"
     CDS             5982..7040
                     /locus_tag="PC471_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012101107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HDF2788921.1"
                     /translation="MGNKMRASVLYNVGDVRYEMVNIPEITDTQVLVNVKYVGICGSD
                     LPRSMVSGLSGNTKYPLILGHEFSGEVVKIGEKVKHINVGDRVAVAPLVPCGKCDYCN
                     EGNFGLCDDYNIIGTRVNGAFAEYVRVPEEHILKLPDTLDYETAAGIEPATIAYHGIS
                     KSNIRVGDSVVVLGCGPIGQFVIQWAKVFGASKIIAVDIFDEKLELSKLLGANYILNS
                     KEVNVIKEIKKITNGGADVVIETAGSRFTQEQSLFVAKKRGNIVFVGISHTELPLSAD
                     ATECILRGELTLKGSWNSYTSPYPGRAWTATLDFMEKGDIIFKPMISDKIGLNEVGDF
                     LSKMSKREINFNKILVEI"
     gene            7139..7825
                     /gene="araD"
                     /locus_tag="PC471_001791"
     CDS             7139..7825
                     /gene="araD"
                     /locus_tag="PC471_001791"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HDF2788922.1"
                     /translation="MLENLKEEVLKANLELPKKKLITYTWGNVSGIDREKGLVVIKPS
                     GVEYSNMTVEDMVVVDLEGNIVDGKLRPSSDTPTHLVLYKEFEELGGIVHTHSSWATI
                     WAQIGKSIPSCGTTHADYFYGSIPCTRKMTREEICNEYEKETGNVIIEEFMGRNPIHC
                     PGIIVNDHGPFTWGKDANEAVHNAVVLEEVAKMAYYTELMSPDNIMDKVLMNKHFSRK
                     HGKNAYYGQK"
     gene            7843..8490
                     /locus_tag="PC471_001792"
     CDS             7843..8490
                     /locus_tag="PC471_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HDF2788923.1"
                     /translation="MLDSLKGKVVEIAIKAEHYGLCKEKTGNFSIRDKDTGYILITPS
                     GVGREELKVEHICVLDLDGNVIEVEKGIKPSSEVLMHIEIYKTRENINAISHTHSLYA
                     TAFSVAKKPIIPIVYESVNYGGYVDIASYERPGTLKLAKSVSKLLIDTDACLLERHGL
                     TTVGNDLDEALLKSRYVEEVAEIYYRSLVLNQFKELEVVDVDEFKSWKYPEEIKF"
     gene            complement(8663..10030)
                     /locus_tag="PC471_001793"
     CDS             complement(8663..10030)
                     /locus_tag="PC471_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HDF2788924.1"
                     /translation="MNLIDILNKVDAFIWGPPLLVLLVGTGILLTVKLGVVQITKLPR
                     ALKLIFSAENKGSGDVSSFAALCTALAATVGTGNIVGVATAIKAGGPGALFWMWIAAF
                     FGMATKYSEGVLAIKYRTKDKNGQVSGGPMYYIVNGMGEKWRPLAIFFAISGILVALL
                     GIGTFTQVNSITDAINNSFGIDPRITGVVLAVFVALVVFGGLKSISNVATKIVPFMAV
                     IYVVICGIILISFWNKIPETFMLIIKSAFTPTAATGGFLGATMSLAIRNGIARGVFSN
                     ESGLGSAPIAAAAAKTEWPAEQGLISMTGTFIDTIIICTLTGFSLVISGVWCSDLNGA
                     VMTQAAFNGAIPTFGPILLTVSLTLFAFTTILGWSYYGERCFEFLFGVKGMNGYRTVF
                     VAMVLLGAFLKLEVVWIIADIVNGLMAIPNLIALLALSPIIVSETKKYFEHINSPENQ
                     IKKNA"
     regulatory      complement(10220..10308)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            10736..12856
                     /locus_tag="PC471_001794"
     CDS             10736..12856
                     /locus_tag="PC471_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HDF2788925.1"
                     /translation="MGKILVLAEKPSVGRDIAKVLGSKNEKNGYIEGPKYVITWALGH
                     LVTLADPESYGERYKSWSLEDLPILPKHLKTVVIKKSGKQFNTVKSQMNRNDIDEIVI
                     ATDAGREGELVARWIIEKSQVKKPIKRLWISSSTDKAIKDGFAKLRSGKEYENLYYSA
                     IARAEADWIIGINATRALTTKYNAQLSCGRVQTPTLAMLLKREEEIRNFKPKEYYGLE
                     LIATKGNSDIKFIWNDKNNNSSTFSKEKIESTLKKVKGVDIKISEVNKSYKKKYSPAL
                     YDLTELQRDANKMFGFSAKETLSIMQKLYEHHKVLTYPRTDSRYLTSDIVGTLKDRIK
                     AVNISDYSKICNRLLKSNIIANKSFVDNSKVTDHHAIIPTEQKVFMSDFSDKERKIYN
                     LVVKRFLAVLSPPFEYEQTTISATVEGEIFKAKGNKVINLGWKESYKDIEDEDEYESI
                     PNVLEKDVLKVKGLKITSGKTNPPPYLNEGTLLTAMEKNGLGTVATRADIIEKLFNSF
                     LMEKKNKDIYITSKGRQLLDLVPVDLKTPELTASWEKKLLDISKGKLSKNTFINDMKD
                     YSKDIVLEVKNSDNKFKHDNLTKNRCPECGKFMLEVNGKKGKMLICEDRECNTRKTIS
                     QVTNSRCPNCHKKLELRGEGEGRLFTCSCGYREKLSSFNKRKQEEKQKASKKDVNQYL
                     KNQNKNQETFNNPFAEALAKLKNK"
     gene            12960..14213
                     /locus_tag="PC471_001795"
     CDS             12960..14213
                     /locus_tag="PC471_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FliS"
                     /protein_id="HDF2788926.1"
                     /translation="MDLSGIFKYYCKECENTWNNSSVELFENIETYSKDSQKKREKEL
                     DKLLNTISVHLERYPSDAVLRKMWVKKGEVFLQKTLEKENIFKLEKMDVEDRKKFLDI
                     TKQFIRDARKFDDDLPIGDIMQAMRNVWISNALQLLFGKEVYYSKANFAYSMLYPYTD
                     NYLDNTNIDKNDKILFNNWLEKRLLGEHIKSKDYHESKVSQMIDYIESVYPREKFTEV
                     YESLLLIFKSQVNSLKQHGKENHLCKEDLLSISIEKGGSSVLVDGYLISGLMTKEEIE
                     FCIGYGFLLQISDDLQDIKEDLKYNHKTIITEMSKEGTLDKVVNKLINFTIELIDSFK
                     INNKNKSVIAMIKNDCLMLILFSVVYNAEFFSVGYIKEVEKFIPYTIDYSLEIEEKIK
                     EKFKNIDVLNNENEYKEMIDIICAE"
     gene            complement(14367..15428)
                     /gene="ytvI"
                     /locus_tag="PC471_001796"
     CDS             complement(14367..15428)
                     /gene="ytvI"
                     /locus_tag="PC471_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation integral membrane protein YtvI"
                     /protein_id="HDF2788927.1"
                     /translation="MDIQKQKNFIIKFTYVVLITSIVYIVLKFLLPLLMPFVISFIIA
                     SILRPIIRLITNNTNLNRTLISIVILLIFYGLCIFLLVSFGVKIFASVSDVFFRLPEI
                     YKSSIQPTLNTLFSKIDASTPNVNLALILGWDNISQSMMSLVASISTNALNAIASIAS
                     KTPAFMLKLIITLIASFFFTFDYQKIVNFILKQFPEKSQFMIINIKNSSINALLKLLK
                     AYAILLSVTFIELLIGLTILKVENAFTISVIIALVDILPVLGTGSILTPWMIISLING
                     NINLAIGLLILYIIITVVRQILEPKVVGHQIGLYPLITLMCMFVGAQLFGIAGLFGFP
                     IAATIIKNLHDNGIITAFK"
     gene            15702..16442
                     /locus_tag="PC471_001797"
     CDS             15702..16442
                     /locus_tag="PC471_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein phosphatase"
                     /protein_id="HDF2788928.1"
                     /translation="MKNFRDLGGNKTEDGRTVKKGLFYRSAKLSNLSENDIKILKELN
                     IKYIFDYRSDEEARKHPSTIISNIKNIRIPAMRELEESGGSFGSIEDMIDGLFEKDGA
                     FNMLNNSYYNLPINNPSYKKLVELIRDYSNLPILNHCTAGKDRTGVGSAIILMILGVS
                     RENIMKDYLKSNDFADKEIERFIEYKPKFKDIPKENLKYIFGVNEEYMKTAFRRIDEE
                     YISVEAYLYGEFNLNKEEIRKLRNQYLE"
     gene            16454..18232
                     /locus_tag="PC471_001798"
     CDS             16454..18232
                     /locus_tag="PC471_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HDF2788929.1"
                     /translation="MSLNITARQINIIKILLNSKDTISGIALSQEIGCSSKTIQNEIK
                     DINKQLRNGKILSIRGVGYKLEGSFDEVNLNSNLYDDVDRVEYIIRKILTLSNEEKNT
                     IKLEDLADSMYVSVSTVKNDLKEVKRMLEKYSISVKSKHKQGICVLESEDKILNCIVD
                     LSNKRDNQLSLNDFLNDDIKERKLLIKKLLLDILNENGLVLTDIEFKSVLNNILVMLS
                     RHKYNEKEFIENYIENYRSKRSSILENEQNKEIIINSIKTFCKNLKLATSIDISNDKV
                     FEEFLYKHISSLCKKIKLGINQSPIMAQDIKIKYPFAFELANIAKKTIEKDLKIEINE
                     DEVANIALHIGGAVERASYNNKDKVFKTIIVCTSGIGTSMLIKAKLENIFKEKLEILK
                     IIPSYLVEYIKVIDVDFVISTVPIEVGDVPVINISPILSEKEIRLIEKYIETGNIYLD
                     LDIKSLFDSELFFTDLVFKRKEEVIDFMASKLVQKGYIDEDMKKSYFEREKIATTEIG
                     NMVTIPHGAIGKIFDNKIAIGILKKPISWEISDVRLIIMLALDKDKILDYEVIFSKIY
                     KRVDSIAKVISICENSSFEKFVNLFN"
     gene            18399..19316
                     /gene="pfkB"
                     /locus_tag="PC471_001799"
     CDS             18399..19316
                     /gene="pfkB"
                     /locus_tag="PC471_001799"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HDF2788930.1"
                     /translation="MIYTVTLNPSIDYIVKLDELKTGSTNRVKEEYVYPGGKGINVSR
                     IFKELGNDNTSLGFISGFTGEYIIRTLEEKELKTDFIKIKSGFSRINVKIKALKETEI
                     NGQGPNIDDEDIDTLYKKLDKLNQDDILILAGSIPSTLDEKLYENIMARLEKKNIKVV
                     VDATKNLLLNVLKYKPFLIKPNNDELEELFGVKLNSIEDMVKYARRLKEMGAINVLVS
                     MGKDGALLITEEDEVLISDVPKGKVKNSVGAGDSMVAGFISGYLNTGKYDYALKLGAA
                     SGSATAFSYDLAKREYIDKLVNEISVKQF"
     gene            19353..21272
                     /locus_tag="PC471_001800"
     CDS             19353..21272
                     /locus_tag="PC471_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDF2788931.1"
                     /translation="MKIIDLLDEKSIKLNLNSKTKSEAIEELVDLVANSGNLNDKENY
                     KKAILAREEMSTTGIGEGVAIPHAKNSSVTKACIAAAVSKEGIDYESFDGSLSNLFFM
                     IAAPDGANNTHLEVLSRLSTILMDEDFRNKLINSSSEKEFKEIIDKKEREKFSEEYQD
                     EKVTEKNIIDTKENDANKYPKVLAVTACPTGIAHTFMAAESLNKMAENKGVSIKVETN
                     GSAGVKNKLTKDEIENATCIIVAADKNVEMARFNGKKVIKTKVADGIHKAEELIDKAV
                     NGDAPIYHGGDGSHNESNEESESGFRKVYKHLMNGVSNMLPFVIGGGILIAIAFLLDD
                     YTINPSNFGSNTPIAAFFKGIGDKAFGFMLPVLAGYIAYSISDRPAFVVGFVGGALAG
                     DGGSGFLGALLAGFIAGYLVEGLKKIFSVLPASLEGIKPVLLYPLLGTLLMGIIMTFL
                     VIPPVTAINNAMVGFLNGLGGTSKIVLGLVLGGMMAVDMGGPVNKAAYVFGVASLESG
                     QFEIMAAVMAGGMVPPLAIALATTFFKNRFTKEERDSGKVNYVMGLSFVTEGAIPFAA
                     GDPLHVIPACVGGSAVAGALSMLFNAALRAPHGGVFVIPVVTHPFAYILAIAVGALVG
                     MMLLALLKKPLNQEV"
     gene            21868..23394
                     /locus_tag="PC471_001801"
     CDS             21868..23394
                     /locus_tag="PC471_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HDF2788932.1"
                     /translation="MNESYWFLNSSPKEYNKLGENIKTDCLIVGGGITGLTTAYLLAK
                     EGKKVVLVEADKIGYGTSGRNTGKVTCQHDIFYSKIEKKYGLDKAKSYYNANNEALNL
                     VEQIIEENNIKCDFKRETSFVFTEKEDTIKDIKDEYRTCKKIGINCEYHETIENIPLD
                     IKGAISFTNQGQFNPKKYIDGLAKAAVNLGLKIYENTPVVDLEKGKICRVKTREDNII
                     EAENVIISSHSPWYDGLNFYFAKEYAERAYLMVAVLENKLADGMFISIDDPSITFRQY
                     NDGSENLLIFGGGDHKVGQGGTEKEIFDDLEHYGKEVFKVKDFKGKWSAQDNMSFDNV
                     PYIGYINKREDNIYVATGFSKWGITNGTAAGIIIKDLIINNNSDYKDTFNPSRLGSYF
                     SKDFIKENANVAINYVSGKLKIGSGDMPKNNGEGKIVNIDGKRYGVYKDDNGDFYIVD
                     TTCTHLGCELNFNSEEKTWDCPCHGSRFDYKGNILEGPALKPLKLYGHGDNDVNPKLL
                     "
     gene            23422..23979
                     /locus_tag="PC471_001802"
     CDS             23422..23979
                     /locus_tag="PC471_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2788933.1"
                     /translation="MNKLLKPITILLYIVYILSAVFFVANKEYGSLGLVAISLVGTFI
                     LSFINKQTERLLSKELYIMLALFIMFSSLLGTCFNFYDINYYDDFLHLWSGIISCIVA
                     FSIIKYFYTQREISKMSKVFLVIFLFMFSMGVASLWEIGEFLMDTFVGTTTQAGGLED
                     TMIDMVDALIGTIITIPFIVKKSNI"
     gene            24501..25538
                     /locus_tag="PC471_001803"
     CDS             24501..25538
                     /locus_tag="PC471_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2788934.1"
                     /translation="MLKKWFGIVKKRQKSESVKEEGEVILKNEKILSEEKLIDEEGVV
                     VNIDNQILTKVVNDDNEIEEKIIEEDLLISENTIKLDDKEAIINDKNIELCKKEVQVE
                     SEKIGLNKFEGLDQNENYNLEKNVIEEKKVSECLTEEDLEYIKEIKIKRGKSIKAINL
                     YTKEEWVFDTHIQCSKKLKVPLGYIRENLKYGYMDYFGDAINYLSEVLNIDEYCKSEW
                     SYLDNSKSPSEIFNILNNKIFSIRLSNEKRNEILTNDKIEALKMNYRFECIDEEYDEY
                     FKKYKSIIKRGGKKKVELVNKKGDILEIFKSLEECAIYLQKEKNEVIQMLKYGDTKVG
                     RNFIRYSLRSI"
     gene            25649..26791
                     /locus_tag="PC471_001804"
     CDS             25649..26791
                     /locus_tag="PC471_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HDF2788935.1"
                     /translation="MSEEKNPKENTLDESKNSDNVNSKKDDVSDEKNTDNKVKKREIK
                     LNNKTLKAIVAIVAVGAICLGGGYVFGKEAGRKLPATSKHYNSSEVVATIGDKKITGE
                     MLRKRMEPLFYLNAKTTMTDDEIRSYESNMIETMITSDILSSEAAKEKISITNDQLDA
                     QYNNLMSSIESAMGMTKEEFLKQFDLTEKYIKDDMKKELVASKYLNEKSKVTDKEAEN
                     YYNKNKSNYLQVRASHILIKTVDDKGKQVSSSKKAELKKEAEEILKKAQAGEDFATLA
                     KKYSEDSSAESGGDLGFFGKGQMVESFEKAAFALKKGEVSNKLVESDYGYHIIKKTDE
                     KYQPFEEVKSDLVSSLTSEKQNLLIQNLKEKYNVKITDKYQMKAKE"
BASE COUNT        10912 a         2747 c         4712 g         8524 t
ORIGIN      
        1 gtttttcaat cataaattta taaagtattt tgcattaaaa tttaaacaaa gaattaaatt
       61 atttaagatt atgaataact aatacattta gtaaattact aaatatacaa aatgtaaata
      121 cttatcaaaa tgtataaata cttatgacat ctagttactt acagggttta agtagaaaaa
      181 aatatcaaag taagtatggc atgatagttg cttatatatt tatcaagaaa ggagttatta
      241 agcatggagt tgagagataa acttgtaaac atcataaata cagaaaataa aaaagatcca
      301 ttaacagatg ttcaaatagc aaagttttta agtacaacta gagagaacat aactaatttg
      361 agaaaagaac ttaatataag taattcaagg caaagaagat atccttattt aaaatcagca
      421 atttcggcaa tattacaaaa gaataagaat atttctatat cagaaattac aagggaattg
      481 atgacagaag gctttaatat atcgagaaga gttgtggagg aacttttacc taaagaaagt
      541 ttagaaatgg atgtagtaga ggaagatgaa gaagaatcga atgacccatt tgaaactctg
      601 ataggtaata aagggagtct tagaaatgct gtagagcagg ctaaatctgc tatattgtat
      661 ccaccaaaag ggttaccaac gttgattatt ggggaaagtg gagttggtaa gagtttattt
      721 tctagacata tgtatgagtt tgcaaagcaa aaaaagatag taaaagagag ctccaatttt
      781 gttgtattta actgtgctga ttatagtgac aatccacaat tattattatc tttgttattt
      841 ggatataaaa aaggtgcatt tacaggtgct gaatatgata caccaggtct tgtagaagaa
      901 gctgatgaag gagttttatt tttggatgaa atacatagat tgcctccaaa aggtcaagaa
      961 atactatttt ctatcttaga taggggaaag tttaggagat taggtgaaac taatgctgaa
     1021 agaaaagtaa gtattatgtt gattggggca actactgaaa atgtagagac taatttatta
     1081 ttaacattca gaagaaggat accaatgcta ataactttgc caccattaca tgatagatta
     1141 ctaaaagaga aaatagacct tatatacaat atctttcaac aagagtgtaa tagaataaat
     1201 gcaaaaatat ttgtagataa aaatgtgatt gaaatacttg cactaaaaaa gtttagtgga
     1261 aatataggac aattacaaaa tatgattcag gttctttgtg caagagcttt tatgaagttt
     1321 ataaattcca aagatgaaga tagtgaatct atagtagtag tagatataaa tgaagtttta
     1381 aagcttaaag attcgtttaa agatgctgcc tttcaagaga ttgaatatac agagataaga
     1441 aaatatttga aaaatgctat atttatacca tttaatttag aagaatcatc agtaaaagga
     1501 agtcttttaa gtgatgaata tatattacca gaggacatat acaaaacaat tgaaaagaaa
     1561 tattatgacc taaaatccct agacattgaa gatatagata tagagaatat tttatggact
     1621 tttattttaa atagatttgc tgacttaaag tttaatcttg atagtaatga agaaattttt
     1681 tcaatgagtg atttaaatag ttttgtaagt gatagtttag ttaaactggt aaaagaactt
     1741 atgaatgata tagttgaaaa aaatatgaat ttagaagtaa ataaaaatat gttcaaatat
     1801 ctagcaatac acttagaaga agctataaaa agaattaagc taaatcaaaa gataataaat
     1861 gtaaatttag aaaaaataaa gattgacttt tcaaaagaat atgaaattag taaaagtttt
     1921 gcaataaaaa ttgaggaatc aattggtatt aagatttcag atgatgaaat tggatttata
     1981 acgctctaca taaagtctgc tctaaaaaat gaagtgaaaa aagataaagt agggttaata
     2041 ataatttctc atggacgtat agctacagag actgtaaatg tagttaaaga acttttaggt
     2101 gtaaagtttc ctgtggctat agatatgcct ttagatgaaa aaccaataaa tatatacaat
     2161 aaggcagtag aattgtcaaa aataatagac caagggaaag gcatattgtt tttggtggat
     2221 attggttcac ttacaaacat aggtcaaata gtaaataaga gaaccaatat aagtactaaa
     2281 actattgata gagtagacct cctaatggca ctagaagcaa ctagaaaagt ttctatggga
     2341 gaggaagaac ttgatgaaat atttttttct ttgctaaaag ataggatggg atataactat
     2401 gaattagaaa aacacataga taaatcaaat gcaattataa cattatgtct tactggagaa
     2461 ggaactgcta agtatataag taaaacgttg gaagaaaagt atgataacac taaatgttat
     2521 caaatgagtg cattggatga aagtttattt tgtaaaattg aaaatctaaa agagacaaac
     2581 aatatattag ctattgttgg gacaataaac cctaagatac caggtataaa ttttataccc
     2641 tataataaag aacttttcaa aaatttggat atctatctat caaaatcaaa acaacaagat
     2701 gacagaccaa ttaaatcgta tgaaagaatg cttgatgagg atttagtgat ttttgaacca
     2761 gatatatatt ttaaaaagga tttattagaa tatgtatgtt caattcttat aaataaagaa
     2821 tatgtagaaa aagattactt agattctgtt ttacatagag aagaaatgct tccaacatat
     2881 tctaaaggag ctataggtgt gcctcatgga gattcttcta ctgtaaatag tacaaggttt
     2941 gtttttgtta aactaaagaa tcctattgat tggggtgtag gtaatgtaaa tttcatattt
     3001 atgccagttt ttcaggctaa tgataaagag attgttaaaa atgttcttga gatactaaaa
     3061 gatgcagaat ttatgaacaa tgccaataag tgttttgata aagatgcttt tagaaggatt
     3121 atatttgata aatttaaaca tctgtagcca tagcaaaaaa attgagagga gttagagtaa
     3181 tgaatgtaga tttaatatca ttgggtttag aatctaatga ttatgaaagt gttattgaag
     3241 aattaggaag tataatgtgt aaaaaagaat atgtaaaaga aacatacata aatgctgtct
     3301 tggaaagaga aaggacatta ccaacaggtc tagatattgg agacatgtgt gttgctattc
     3361 cacatacaga ttcaaagcat gtaaatgagt ctaatgtagc tgtagggata ttgaaaaatc
     3421 ctgtaaagtt taattcaatg atagacccaa aagatagact tgatgtagaa cttgtatttt
     3481 tattagcagt aaagaaccca gactcacaag taaaactgtt aaaagattta atgtcagttt
     3541 ttcaaaatat aaagttatta aaaaatataa aaaacgcatc aacaaaagaa gaagtagcta
     3601 aattattgga ttttatagaa atttaatgta ggagaatttt ataaataaaa aattaaaatt
     3661 taaacaatca aagtaagtat tattttttta ataggtttaa tcttatataa aataaaaatt
     3721 tagagtgaaa aattaggagg aaattattat gaaacaattt aatgtattat cagtttgtgg
     3781 ttcagggaca gttacatcat ctatggtagc aggtaaatta aaagaagtac taggtaataa
     3841 ggggatttct attactacaa cagaagctag accaacagaa gcactaaatt tagcacaatc
     3901 aggaagattt gactttataa catttacgag tccacttcaa acaggcgatt atggaatacc
     3961 aactataaat gcatttgctt gcctaactgg tataggggaa gaggcattct ttgaagaagt
     4021 tttgactgtt attgaaggga tagataagta agttttggag acgtgaattt atagtattta
     4081 aatcaatgat ttagatacta taataagcta atattaacta aatatttgtg gaaaaaacat
     4141 tttatatgtt gtcataaatt aaaaaattat aaaaatagtc acttaagtat taaattaagc
     4201 ttataaatat ttataagtag gagggaaaaa gatgttagaa tttatgaaat ctataatcga
     4261 tacatttggt tctgcaatta tagttccaat tataatattc ataatcgcaa agatatttaa
     4321 agtaactact aagaaatcgt ttctttctgc tgtgtatgca ggagttgctt tacaaggttt
     4381 tacactgatt ttaaattcat ttacaccagt aataacacca gttatcaata ggatggttga
     4441 gagtacagga gtaaacctac cagtttttga tgttggttgg caagcaacat cattggttgc
     4501 attttcaaca agtgcaggta tgatatattt aggtcttggt atattattac aaacaatttt
     4561 atttttgata aaatggacag atgtgtttca accatcagat ttatggaata actattctta
     4621 tatggtatgg ggagcaatgg ttataggagt aacaggaaat tttcctttag gtatagcatg
     4681 tatggtttta ttaaacctat atagtttatt aatatcagag ttagttgcaa agagatggtc
     4741 tagttactat agatatccta attgtacaat aatagctatg cataatgttg aggcttctgt
     4801 gtttgcagta tttgcagacc ctatatacaa taaattggga ttaaataaaa taaaattaaa
     4861 tccaaaagaa ttagagaaga aattagggtt tttaggtgaa ccaattactt taggtctttt
     4921 tttaggtatg tttataggta tattaggtaa tatgacgaga ataaatacta tggaagcttg
     4981 gggagaaata atgaaagtag gtatatctac aagtgcagtg atggcaattt tccctaaagt
     5041 agcaagtatg tttgctcaag catttgcacc tataacagaa gcagctagaa agattatgca
     5101 aaaagctgga aaccgtgagt ggtacatagc agtaaatgat gctgttggat atggtgaacc
     5161 tgcaacttta atatcaggtc ttatattaat tccaataatg cttgtaatag ctatggtact
     5221 tcctggtaat aaggtattac cagttgtgga tttattggca attccatata tggttcaagg
     5281 tttagttgcc atacataatg gtaatatacc aaaagtatta gtttcaggta taatttggtt
     5341 tggattagga ttatatgttt gtacatctac agcaccatta tttacagata tggctacaaa
     5401 tataggtgta gctatacctg caggagcaat gttaataaca agttttaata tactaggaaa
     5461 acctttgatg ggattagttt tctttgcatt tctaagtgca aatccaattt atataggtct
     5521 ttcagttgta atttactttg ttctatgggc attgtttaga aagaacaaaa caagtattct
     5581 tgattattta gaaaaacaag cacttaaaaa tgtagaagaa gagcctgtag cagtttaagt
     5641 ttataaaaat gaaataaaaa taattaaagg agtttataat tgattttata taagtatcag
     5701 ataatacctg agaaatattt aaatgaaata tagacttctt tttatttgat aatatttgta
     5761 attataatgc attgtatttt tatagttgag ttttctgtat aacctgctat ttaaaacata
     5821 ctcttttata tttatacagt aatgtggttt aattttataa aatgtaatct ctactggtaa
     5881 aagactttgt atataaggca tttgcctgaa taaaccattt atcttttgta tggcatagat
     5941 attgctatat agaagtatat agcatgaaag gagatattta tatgggaaat aaaatgagag
     6001 catcagtatt gtataatgta ggtgatgtca gatatgaaat ggtaaatata ccagaaataa
     6061 cagatacgca agtgcttgta aatgtaaaat atgttgggat atgtggttca gatttaccta
     6121 ggtctatggt aagtggtttg agtggaaata caaaatatcc attgatttta gggcatgaat
     6181 tttctggtga agttgttaaa attggagaaa aagtaaagca tattaatgta ggagatagag
     6241 tagctgttgc accattagta ccttgtggaa aatgtgatta ttgtaatgaa ggtaattttg
     6301 gcttgtgtga tgattacaat ataattggaa caagagtaaa tggagcgttt gcagaatatg
     6361 taagagttcc agaagaacat attttaaaac taccagatac attggattat gaaactgctg
     6421 ccggaattga accagcaact atagcttatc atgggatttc aaagtctaat attagagtag
     6481 gagatagtgt agttgtgtta ggatgtggtc caataggtca gtttgttata caatgggcaa
     6541 aggtgtttgg tgcatctaag attatagcag ttgatatttt tgatgaaaaa ttagaacttt
     6601 ctaaacttct aggagcaaac tatatactta actcaaaaga agtaaatgta atcaaagaaa
     6661 taaaaaaaat aacaaatggt ggtgctgatg ttgtaataga aactgctggt agccgattta
     6721 cacaagaaca atctctattt gtagcaaaaa agagaggaaa tatagtgttt gtaggtatat
     6781 cacacacaga attaccactt agtgcagacg ctactgagtg tatattacgt ggagaactta
     6841 ccttgaaagg ttcttggaat tcatatacaa gcccttatcc tggaagagca tggactgcaa
     6901 cattggattt tatggaaaaa ggtgacatta tttttaaacc catgatttca gataaaatag
     6961 gtcttaatga agtgggtgac tttttaagta aaatgagcaa gagagaaatt aatttcaata
     7021 aaatcctagt agaaatataa aaatttaata aaaaattcta aaagcaatta aattttataa
     7081 ctatgacatt aaataaaata aaaataattt tcttaaataa aatataggag gaagaaaagt
     7141 gttagaaaat ttaaaagaag aagtattaaa agcaaactta gaattaccta agaaaaagct
     7201 aattacttat acttggggaa atgtaagtgg gatagataga gaaaaaggat tagtggtgat
     7261 aaagccaagt ggtgtagagt atagtaatat gactgtagaa gatatggttg tagttgattt
     7321 agagggaaat atagttgatg gcaaattaag accttcttca gataccccga ctcatctagt
     7381 tttatataaa gagtttgaag aattgggggg aatagtacat acacattcaa gttgggcaac
     7441 aatttgggct caaataggaa aatctatacc atcttgtggt actactcatg cagattattt
     7501 ttatggaagt ataccatgta ccagaaaaat gactagagaa gaaatttgca atgaatacga
     7561 aaaggaaact ggaaatgtaa tcattgaaga atttatggga agaaatccaa ttcattgtcc
     7621 aggaataatt gtaaatgacc acggaccttt tacatgggga aaagatgcaa atgaggctgt
     7681 acacaatgca gtagttttag aagaagtcgc aaagatggct tattatacag agttaatgag
     7741 tcctgataat attatggata aggttcttat gaataagcat ttttcaagaa aacatggaaa
     7801 aaatgcatac tatggtcaaa aatgattatc tggaggtgaa ttatgctaga ctctttaaaa
     7861 ggtaaagttg tggaaatagc aattaaagca gagcattatg gtttatgtaa agaaaagact
     7921 ggtaatttta gtattagaga taaggatact ggatatatac ttataacccc atcaggtgta
     7981 ggaagagaag aattaaaagt tgaacatatt tgtgtattgg atttggatgg aaatgtaata
     8041 gaagttgaga aaggtattaa gccaagtagt gaggtattga tgcatattga aatatacaaa
     8101 acaagagaaa atataaatgc catatcacat actcattctt tatatgcaac agcattttca
     8161 gtagcaaaga aaccaattat accaattgta tatgaatcag ttaattatgg cggttatgta
     8221 gatattgctt catacgaaag accaggaact ttaaaattag ctaagagtgt ttcaaaatta
     8281 ctgatagata cagatgcatg cttattagaa agacatgggt tgacaactgt aggtaatgat
     8341 ttagacgaag cattattaaa atcaagatat gtggaagagg tagcagaaat atactatagg
     8401 tctttagtat taaatcaatt taaagaactt gaagtagtag atgttgatga gtttaaatct
     8461 tggaaatacc cagaagaaat aaagttttaa tctaaataat tttcattgaa gtggttaatt
     8521 aaaatatatg tattatttat atcaattact atgttttatt atgttaatta ccgtattttg
     8581 caaatatatt tattacattt tttcaaaaaa atatagaatc cgaaagccat ataaaattga
     8641 cttgggattc tatacttttt atttatgcat ttttctttat ctgattttct ggagaattta
     8701 tatgttcaaa atatttttta gtttcagata ctattatagg tgataatgca agtaaagcta
     8761 taaggttagg tattgccata agcccattta caatatcagc aattatccaa acaacttcta
     8821 actttaagaa agcacctaat aacaccatag ccacaaatac agttctatac ccattcatgc
     8881 cttttacacc aaacagaaat tcaaaacatc tttctccata ataactccat cccaaaattg
     8941 tagtaaatgc aaataatgtt aaactaactg taagtaatat tggtccaaat gtaggaattg
     9001 ctccgttaaa agctgcttgt gtcataacag ctccatttag gtctgaacac cacactcctg
     9061 aaataacaag agagaatcca gtaagagtac agattataat agtatctata aaagtacctg
     9121 tcatagatat aagaccttgt tccgctggcc attcagtttt agcagcagct gcagctattg
     9181 gagcactacc tagaccagac tcatttgaaa atacaccacg agcgatacca tttctaattg
     9241 ccagtgacat agttgctcct aaaaaaccac cagtagcagc agtaggtgta aatgcacttt
     9301 taattattaa cataaaagtt tcagggattt tattccaaaa agatattaaa atgattccac
     9361 agattacaac atatataact gccataaaag gtactatttt agtagctaca ttagatatac
     9421 ttttaagtcc tccaaataca actaaagcta caaatactgc taatacaacc cctgtaattc
     9481 tagggtctat cccgaaacta ttatttattg catctgttat tgaatttact tgagtaaaag
     9541 taccgattcc taaaagtgca actagtatac cacttatagc gaaaaatatt gcaagaggtc
     9601 tccatttttc tcccatacca tttactatgt aatacattgg cccaccagac acttgaccat
     9661 ttttatcttt agttctgtat tttattgcaa gaacaccttc tgaatactta gtagccattc
     9721 caaagaatgc tgctatccac atccaaaaaa gtgcaccagg tccaccagct ttgatagctg
     9781 tagcaacacc aacaatatta ccagtaccaa ctgtagcagc tagagcagta cataaggctg
     9841 caaaacttga aacatcacca ctacctttgt tttcagcaga gaatattaac ttaagagctc
     9901 ttggcagttt agttatttga acaactccaa gcttaacagt aagtagtata ccagtaccaa
     9961 ctaatagtac cagtaggggt ggtccccaaa taaaagcatc aactttgttt aaaatatcaa
    10021 ttagattcat aaaaacactc cctcattttt tttgctttac ataatcaata gaatttttaa
    10081 tgaagtaatt gacaggaaat aagtataatt tattaataat ttctagtgta ataaaaaaag
    10141 ctaaatctaa aaaagattca gctctggatg tacactaatt agcaaccaaa ataaaagtat
    10201 tagtaaatac tgtttttggt agataatccc tgtccttttg cctgagagat tgagcataaa
    10261 agccttgccc cttcggcgcc cataatgggt ctctccagag ttccgtaaat tttacagtcc
    10321 tacttttaaa agtacctgag agtgttactc cttcggtgtg ctatatagca actcttctat
    10381 aaaatcttaa ccggatattg atttgtcata gcttattgta ttaataatat taatataagt
    10441 ttttatgttt tgtcaactaa aaaattaaaa aacaaagaaa aaatataaaa atgtaagcct
    10501 atttacgaat tataaactta gaaagatgag taatatacgt gaaaatgaat tttgtaatac
    10561 acatacaaat gaagtgtaaa ataagtttta aataaaaaaa taataaaatc ttaaataatt
    10621 aattagatat gattaaatta tggtaatatt atagttacga tattaagaaa agttctattt
    10681 agttagattt taagcgtaat atgagattat gtatagagaa aggatgtatt tttagatggg
    10741 aaaaatatta gtgctagcag aaaaaccttc tgttggaaga gatatagcaa aagttttagg
    10801 gtcaaaaaat gaaaaaaatg gttatataga ggggccaaag tatgtgataa cttgggcact
    10861 aggtcatttg gttacacttg ctgaccccga aagttatgga gaaagatata aatcatggag
    10921 cttagaagac ctgccaatac ttcccaagca ccttaagact gttgtaataa aaaagagtgg
    10981 aaagcagttt aatacagtta aatcgcaaat gaatagaaat gatatagatg aaatagttat
    11041 agcaacagat gcaggtcgtg aaggagaact tgtagcaagg tggataatag aaaaatcaca
    11101 ggtaaaaaaa cctataaaac gtctttggat ttcttcaagt acagacaaag caataaaaga
    11161 cggatttgca aaattaagaa gtggaaaaga atatgaaaat ttgtattact cagcaatagc
    11221 acgagcagaa gctgattgga ttattggaat aaatgcaacc agagctttga caacaaagta
    11281 caatgcacag ctttcttgtg gaagagtaca aactcctact cttgcaatgt tgcttaaaag
    11341 agaagaagaa ataagaaatt tcaaacctaa agagtattat ggattagaat taattgctac
    11401 aaagggaaat tcagatataa aatttatttg gaatgataaa aacaataatt cttcaacttt
    11461 tagtaaagaa aagatagaat ccacattaaa aaaagtaaaa ggtgtagaca taaaaatatc
    11521 tgaagtaaac aagtcatata agaaaaaata ttcacctgca ctctatgatt taacagaatt
    11581 acaaagagat gcaaataaaa tgtttggatt ttcagcaaaa gaaactttat ctataatgca
    11641 aaaactgtat gaacatcata aagtacttac ttatcctaga actgattcta gatatttaac
    11701 atcagatatt gttggaacat taaaagatag aataaaagct gtaaatatta gtgattattc
    11761 aaagatttgt aatagattat taaaaagcaa tattatagct aataaatcgt ttgttgacaa
    11821 ttcaaaggta acagaccacc atgcaataat accaacagaa cagaaggtat tcatgagtga
    11881 ttttagtgat aaagagcgaa aaatatataa tcttgtagta aaaagatttt tagcagtact
    11941 atctcctcct tttgaatatg agcagacaac tataagtgca actgtagaag gagaaatatt
    12001 taaggctaag ggaaacaaag taataaattt aggttggaaa gaaagttata aagatattga
    12061 agatgaagat gagtatgaaa gtataccaaa tgttttagaa aaagatgtac ttaaggtcaa
    12121 gggattaaag ataacttcag gaaaaacaaa tccaccacca tacttaaatg agggaacatt
    12181 attgactgct atggaaaaga atggtctagg gacagttgct acaagagcag atataataga
    12241 aaaattattc aattcatttt taatggaaaa gaaaaataaa gatatatata taacatcaaa
    12301 aggaagacaa ttattagatt tagttccagt agacttaaag acaccagaac ttacagcttc
    12361 atgggaaaag aaattattgg atatttcaaa gggaaaactt agtaaaaata cattcataaa
    12421 tgacatgaaa gattattcaa aagatattgt actagaagta aaaaatagtg ataataaatt
    12481 taaacatgat aacttaacta aaaatagatg tccagaatgt ggtaaattta tgttagaggt
    12541 aaatggtaaa aaaggtaaga tgttgatttg tgaagataga gagtgtaata ctagaaaaac
    12601 tatatcacaa gtaaccaatt caagatgtcc taactgccat aaaaaattag aacttcgtgg
    12661 agaaggtgag gggagactct tcacttgtag ttgcggatac agagaaaagt tgtcttcttt
    12721 caacaagaga aagcaagaag aaaaacagaa ggcatcaaaa aaagatgtta atcaatattt
    12781 aaaaaatcaa aataaaaatc aagagacatt taataatcca tttgcagaag cattagctaa
    12841 gctaaaaaac aaataatttt aaagttatta ttatacacaa ttataacttt aactgtatat
    12901 tagttatatg tactgatata atatagtgta tagtttaggc atgaaagtag ggctgttaaa
    12961 tggatttaag tggcatattt aaatactatt gtaaagagtg tgaaaataca tggaataatt
    13021 cgagtgttga attatttgag aatatagaaa cgtatagtaa agattcacaa aaaaagaggg
    13081 aaaaagaatt agataaattg ctaaatacaa tatcagttca tttagagagg tatccaagtg
    13141 atgctgtatt gagaaaaatg tgggtaaaaa agggcgaggt tttcttacaa aagacattgg
    13201 aaaaagaaaa tatttttaag ttagaaaaaa tggatgtaga ggatagaaaa aaatttttag
    13261 atataacaaa acagtttatt agagatgcta gaaaatttga tgatgattta cctataggtg
    13321 atattatgca agctatgagg aatgtatgga tttcaaatgc attacaatta ttatttggta
    13381 aagaagtata ttattcaaaa gctaactttg catatagtat gttatatcca tatactgata
    13441 attatttaga caatacaaat atagataaaa atgataagat tttatttaat aactggttag
    13501 aaaaaaggct cctgggagaa cacattaaat ctaaggatta tcatgaaagt aaagtatctc
    13561 aaatgataga ttatattgaa agtgtatacc ctagagaaaa gtttacagaa gtttatgaat
    13621 cgttattatt aatatttaaa agtcaagtaa atagtttaaa acaacatggt aaggaaaatc
    13681 atttgtgtaa agaagattta ttatccattt ctattgaaaa aggaggttca tccgttttag
    13741 tagatggata tttaataagt ggattgatga caaaggaaga aatagagttt tgtataggat
    13801 atggattttt attacaaata tctgatgatt tacaagatat aaaagaagat ttaaaataca
    13861 accataaaac tattattaca gagatgtcaa aagagggtac tttagataaa gttgtaaata
    13921 aactaataaa ttttactatt gagttaatag atagttttaa aattaataat aaaaataaat
    13981 ctgtaatagc tatgataaag aatgattgct taatgttaat tttattttct gtagtttata
    14041 atgctgaatt tttttctgta ggatatataa aagaagtaga gaaatttatt ccatatacaa
    14101 tagattattc attagagatt gaagaaaaaa taaaagaaaa atttaaaaat atagatgttt
    14161 taaataatga aaatgaatat aaagaaatga ttgatattat ttgtgcagag tagaaaaaaa
    14221 gggagtgtct tagaatagac tttttagttt atgaggcact ctttttgtat aaaatcaaat
    14281 gtattttcat cattttaaca attaaaaaga gcttatccct atttgagaaa gcccttttta
    14341 atttaaataa aaatgtcaag gttaaactat ttaaatgcag taataatccc attatcatgt
    14401 aagtttttta tgattgtagc tgcgataggg aatccaaata atccagctat tccaaatagt
    14461 tgagctccaa caaacataca cattaatgtt attaaaggat ataggccaat ttggtgtcct
    14521 actacctttg gttctaatat ttgtctaaca acggttataa ttatgtatag tattaaaagt
    14581 cctattgcta aattaatatt tccattaatg agtgagataa tcatccaagg tgtcaaaata
    14641 cttcctgtac ccaagactgg caaaatatca acaagtgcta tgatgacaga tatagtaaat
    14701 gcattttcaa cctttaggat agtaagtcca ataagtagtt ctatgaatgt cactgatagt
    14761 agtattgcat aggcttttag tagttttaaa agagcattaa tactgctatt ttttatattt
    14821 attatcataa attgactttt ttctggaaat tgttttaaga taaagtttac tattttttga
    14881 tagtcaaaag taaagaaaaa agaagcaatt aaagtaataa taagttttaa cataaatgca
    14941 ggtgttttac ttgcaatact agcaatagca ttgagtgcat ttgttgaaat agaagctact
    15001 aaagacatca tagattgact tatattatcc caaccaagta ttaatgctag gttaacatta
    15061 ggtgtactag catctatctt tgaaaataaa gtatttagcg ttggttgtat acttgattta
    15121 tatatttcag gaagtctaaa aaatacatca cttacacttg caaatatctt cacaccaaag
    15181 ctaactaata aaaatataca caacccataa aaaattaaaa gtattactat agaaataagt
    15241 gtacgattta aatttgtatt atttgtaata agtcttataa taggtcttaa gatggatgca
    15301 atgataaaac tgattacaaa aggcattaat aatggcaata agaattttaa tacaatatat
    15361 acaatacttg ttattaaaac tacatatgta aatttaatta taaaattctt ttgcttttga
    15421 atatccataa tataaaacct ccaatgtatt tgatattatt ctaaatatgg tcttttttac
    15481 agaaaatata ctactgtata ttgattataa actataaagc ttatgtaaag taaataaatt
    15541 atgtaaaatt taggttaatt ttgtataaag agatactttt atattttaca attaataacc
    15601 aaatactaaa aaatatttta aataaagaac ttaaaattgt gataaaatta atttatagat
    15661 attaaaaaaa gtataatata tggtttttaa gaggtgctaa gatgaaaaat tttagagatt
    15721 taggtgggaa caaaactgag gatggcagaa ctgtaaagaa gggattattt tataggtctg
    15781 caaaattatc aaatctaagt gaaaatgata taaagatttt aaaagagtta aatattaaat
    15841 atatatttga ttatagaagt gatgaagaag ctcgtaaaca tccatcaaca ataatatcaa
    15901 atataaaaaa tataagaata ccagcaatga gagagctaga agaatcagga ggaagttttg
    15961 gttctattga agatatgatt gatggattgt ttgaaaaaga tggtgcattt aatatgttaa
    16021 ataatagtta ctataatttg cctataaata atccgtcata taaaaaatta gtagaactta
    16081 taagagacta ttctaattta ccaatattaa atcattgtac agctggtaag gatagaactg
    16141 gtgtggggag tgctataatt cttatgattt taggggtatc gagagagaat ataatgaaag
    16201 attatctaaa aagtaatgat tttgcagata aagagataga aagatttata gaatataagc
    16261 ctaaatttaa ggacattcca aaagaaaatt tgaaatatat atttggtgta aatgaagagt
    16321 atatgaaaac tgcatttaga agaattgatg aagaatacat tagtgtagaa gcatatttat
    16381 atggagaatt taatttaaat aaagaagaaa taagaaaatt aagaaatcaa tatctagagt
    16441 aaaaggtgag tttatgagtt taaatataac agcaagacag ataaatataa taaaaatatt
    16501 gttaaattca aaagatacaa ttagtggtat tgccttaagt caagagatag gatgttcttc
    16561 aaaaactata caaaatgaaa taaaggatat taataaacaa cttagaaatg gtaaaatttt
    16621 gtcaataaga ggagttggtt ataaactaga aggaagtttt gatgaggtaa atctaaatag
    16681 caatctctat gatgatgtag atagagtaga atatataatc agaaaaatat taaccttaag
    16741 taatgaagaa aaaaatacta taaaacttga agatttagcc gattctatgt atgtaagtgt
    16801 ttcaacagta aaaaatgatt taaaagaagt aaaaagaatg ttggaaaagt attcaatatc
    16861 agttaaatct aagcataaac aaggtatatg tgttttggaa agtgaagata agatattaaa
    16921 ttgtatagtt gacttaagca ataagagaga taatcaattg agtttaaatg attttttaaa
    16981 tgatgatata aaggaaagaa aactattaat aaaaaaatta ttattagaca tattaaatga
    17041 aaatggatta gtattaactg atatagaatt taagagtgtg ttaaataata tattagttat
    17101 gttaagcaga cataagtata atgaaaaaga atttatagag aattatatag aaaattatag
    17161 gagcaaaaga agctcaattt tagaaaatga acaaaacaaa gaaattataa taaattcaat
    17221 aaaaacattt tgtaaaaatt taaaattagc tacatcaata gatataagta atgataaggt
    17281 atttgaagag tttttgtata agcatattag tagtttatgt aaaaaaataa agttaggtat
    17341 aaatcaatca cctataatgg ctcaagatat aaaaataaaa tatccatttg cttttgaact
    17401 agctaatata gctaaaaaga caatagaaaa agatttgaag atagaaatta atgaagatga
    17461 agttgcaaac atagctctac atataggagg agcagttgaa agagcatctt ataataataa
    17521 agataaggtt ttcaaaacta taatagtttg tacttcaggc ataggcacat caatgttaat
    17581 aaaagctaaa ttagagaata tatttaagga aaaattagaa atactaaaga ttattccttc
    17641 atacttagtt gaatatataa aagttataga tgttgatttt gtaatatcta ctgttcctat
    17701 tgaagtagga gatgtgccag taataaatat atctccaatt ttaagtgaga aagaaattag
    17761 attaattgaa aaatatatag aaacaggaaa tatataccta gatttagata taaaaagcct
    17821 gtttgatagt gaattgtttt ttactgattt ggtgtttaaa aggaaagaag aagttataga
    17881 ttttatggca agtaaacttg tacaaaaggg ctatatagat gaggatatga aaaaatctta
    17941 ctttgagaga gaaaaaatag caacaacaga aatagggaat atggtaacta taccacatgg
    18001 agctattgga aaaatttttg ataataaaat agcaattggt atattaaaga agcctatttc
    18061 ttgggaaata tcagatgttc gtttaataat aatgttagcc cttgataaag ataaaatatt
    18121 agattatgaa gtaatatttt caaaaatata taaaagagta gattctatag caaaagttat
    18181 aagtatatgt gaaaatagta gttttgaaaa atttgtaaat ttattcaatt agatgggtag
    18241 tattgtatat acacctgtaa atttggataa tataaaacta aataagataa aaacgataaa
    18301 tttctctttt aattaagaga aattttttga ctttcaagga tattaatata aagttaagat
    18361 ataaacataa agcaaataaa taaaaaagag gtggcaatat gatttataca gtgacattaa
    18421 atccatcaat agattatata gttaagctag atgagctaaa gacaggaagt acaaatcgtg
    18481 taaaagaaga atacgtttat ccaggaggaa aaggtataaa tgtatctcgc atatttaagg
    18541 aacttggaaa tgataataca tctttaggtt ttataagtgg ttttactgga gaatatataa
    18601 taagaacatt ggaagaaaaa gaattgaaga cagattttat aaagataaaa agtggttttt
    18661 caagaataaa tgtaaaaata aaggcgttaa aggaaactga gattaatgga caaggtccaa
    18721 atatagatga tgaagacatt gatactttat ataaaaaact tgataagcta aatcaagatg
    18781 atattttaat attggcagga agtataccat ctactttaga tgaaaaacta tacgaaaata
    18841 taatggctag attagaaaaa aagaatataa aagttgtagt agatgcaact aaaaatcttc
    18901 ttcttaatgt tttaaaatat aaaccttttc taattaagcc taataacgac gaattagaag
    18961 aactatttgg tgttaaatta aattcaattg aagacatggt aaaatatgct agaaggttaa
    19021 aggaaatggg agctataaat gtcttggtat caatgggaaa agatggagca ttgttgatta
    19081 cagaggaaga tgaagtgctt ataagtgatg ttccaaaagg aaaagtaaaa aattctgtag
    19141 gagcaggtga ttcaatggta gcaggattta tatctgggta tttgaatact gggaaatatg
    19201 attatgctct taaattggga gcagcttcag gtagtgcaac tgctttttca tatgacttag
    19261 ccaaaagaga atacattgat aaattggtaa atgagatatc tgtaaagcag ttttaaaata
    19321 ttaaaaaata tatagaaagg taattaatat ttatgaaaat tatagattta ttagatgaaa
    19381 agtcaattaa gttaaattta aattcaaaaa ccaaatcaga agcaatagaa gaattagtag
    19441 atttggtcgc aaatagtgga aatttaaatg ataaagaaaa ctacaaaaaa gcaattttgg
    19501 ctcgtgaaga aatgagtaca acaggaattg gagaaggtgt agctatacct catgctaaaa
    19561 attcttcagt tactaaagct tgtatagcag cagcagtgtc aaaagaaggt atagattatg
    19621 aatcgtttga cggaagttta tcaaatctat tttttatgat agctgcacca gacggagcta
    19681 acaatactca tcttgaagta ttatcaagat tatctacaat acttatggat gaagacttta
    19741 gaaataaact tataaattca agctctgaaa aagaatttaa ggaaataata gataaaaagg
    19801 aaagagaaaa attcagtgaa gaatatcaag atgagaaagt tactgaaaaa aatataatag
    19861 atactaaaga gaatgatgct aataagtatc ctaaggtttt agcagtaaca gcatgtccaa
    19921 ctggaatagc acatacattt atggcagctg aaagtttaaa taaaatggca gaaaacaaag
    19981 gtgtatcaat aaaagtagaa actaatggtt cagcaggtgt taaaaataag cttacaaaag
    20041 atgaaataga aaatgcaaca tgtataatag tggcggcaga taaaaatgtt gagatggcta
    20101 gatttaatgg taaaaaagtc ataaaaacta aggtggctga tggtatacac aaagcggaag
    20161 aacttataga caaggctgta aatggagatg ctccaattta ccatggagga gatggaagtc
    20221 acaacgaatc aaatgaagaa agtgaaagtg gatttagaaa agtatacaaa cacttaatga
    20281 atggtgtatc taatatgtta ccatttgtta ttggtggagg gatacttata gcgatagcct
    20341 ttttacttga tgattatact attaaccctt caaactttgg ttcaaataca cctatagcag
    20401 cattttttaa aggaatagga gataaagctt ttggatttat gttacctgta cttgctggat
    20461 atatagcata ctctatatct gatagaccag cgtttgttgt aggatttgta ggaggtgctc
    20521 tagcaggtga tggtggttca ggttttttag gtgcactact tgcaggtttt atagcaggtt
    20581 atttagtaga agggcttaag aaaatattta gtgtactacc agcttcattg gaaggaataa
    20641 aaccagtatt gttataccct ttacttggaa cacttttgat gggaattata atgacatttt
    20701 tagtaatacc tccagttaca gcaattaata atgcaatggt tggattttta aatggtttag
    20761 gtggaacttc aaaaatagtt ttaggattag tattaggtgg aatgatggct gtggatatgg
    20821 gtggacctgt aaataaagca gcatatgtat ttggagttgc atcacttgaa tcagggcagt
    20881 ttgaaataat ggcagcagta atggctggtg gtatggttcc gccacttgct atagctcttg
    20941 caactacatt ttttaagaat agatttacta aagaagaaag agattctggt aaagttaatt
    21001 atgtaatggg attatcattt gttactgaag gagctatacc ttttgcagct ggagacccac
    21061 ttcatgttat accagcttgt gttggaggtt cagcagtggc aggagcactt agtatgttgt
    21121 tcaacgcagc tcttcgtgca cctcatggag gagtatttgt tataccagtg gttacccatc
    21181 catttgcata tatattagct atagctgtag gtgctttggt tggaatgatg cttttagcat
    21241 tacttaaaaa accattaaat caagaagtat agcaattaaa ggatattcaa gaacttaaaa
    21301 tatgatgttt tataaaaatt aatatattgc tgaattattt taaaataagg attttatgag
    21361 gctgttatat aacgatttgt aattgttatc ttgacagtct tttattttta tataagtaaa
    21421 ataagtgtgt tttgctttat ttgtatttaa attgttttta agttagaata taaagaaaat
    21481 tatatattat tttaatattc actcgaacta gaaaataata tttgttgtat ttttttgtat
    21541 ataaaatata ttgtaatatt aaaaaaataa tagtaaactg attgcataaa aatgattttt
    21601 atataaaatt taagcagaag aataaatttc taacgaaatt ggatatttct tctgtaaata
    21661 catattcaaa aaaagaatta taagagttta gtcagcagtc gtgtaagtaa taaggacttt
    21721 ataaatatat agaaatataa tgatattaat ataaccaagc gatttttaat ttagcttttt
    21781 aataagctta ttttttatac tatttataat aaataaactc catgatggga aaactattac
    21841 tatttaatat gtaaaggagt attttaaatg aatgaatcat attggttttt aaactcaagt
    21901 ccaaaagaat ataacaaatt aggtgaaaat ataaaaacag attgtcttat agtaggtggt
    21961 ggaataacgg gattgacaac tgcatattta ttagcaaaag aaggtaaaaa agtagtgctt
    22021 gttgaggctg ataaaatagg atatggaact tctggaagga atactggtaa ggtaacttgt
    22081 cagcatgata tattttactc taagatagag aaaaaatatg gattagataa agcaaaatca
    22141 tattataatg caaataatga agctttaaat ttagttgaac aaataataga agaaaacaat
    22201 ataaaatgtg attttaaaag ggaaacttct tttgtattta cagaaaaaga ggatactata
    22261 aaggatatta aagatgaata tcgtacatgt aaaaaaatag gaataaattg tgagtatcat
    22321 gaaactatag aaaatattcc attagatata aagggggcta ttagttttac taatcaaggt
    22381 caatttaacc caaagaagta tattgatgga ttggcaaaag cagcagtaaa tttagggcta
    22441 aaaatatatg aaaatacacc agtagtagac cttgaaaaag gaaaaatatg tagggttaaa
    22501 acaagagagg ataatataat agaagctgag aatgttataa tttcatctca tagtccatgg
    22561 tatgatggat tgaattttta ttttgcaaaa gaatatgcag agcgtgctta tttaatggtt
    22621 gcagttttgg aaaataaatt agcagatggt atgtttataa gtatagatga cccatcaata
    22681 acttttagac aatataatga tggaagtgaa aatttactga tttttggagg aggagaccat
    22741 aaagttggtc agggaggaac tgagaaagaa atatttgatg atttagagca ttatggtaaa
    22801 gaagtcttta aggtaaaaga ttttaagggg aaatggtctg ctcaagataa tatgagtttt
    22861 gacaatgttc cttatattgg ttatatcaac aaaagagaag acaatattta tgtagctaca
    22921 gggttctcta aatggggaat tactaatgga actgctgctg gaataataat aaaagattta
    22981 ataataaata ataattctga ttataaagat acttttaatc catccagact aggtagctac
    23041 ttttcaaaag attttataaa ggaaaatgca aacgtagcaa taaattatgt aagtggaaaa
    23101 ttaaaaatag gtagtggaga tatgcctaaa aataatggag aaggaaagat tgttaatatt
    23161 gatggaaaaa gatatggtgt atataaggat gataatggag atttttatat tgtagacact
    23221 acatgtactc atttaggatg tgaacttaat tttaacagtg aagaaaaaac atgggactgt
    23281 ccatgtcatg gttctagatt tgactataag ggaaatatac ttgaaggacc agctcttaag
    23341 ccattaaaac tttatggaca tggtgataat gatgttaatc ctaagttatt ataaaattat
    23401 ttttaggaag gtaaagtgta tatgaataaa cttttaaagc caataactat actattgtat
    23461 atagtttata ttttatctgc cgtatttttt gtagcaaaca aagaatatgg aagtctaggt
    23521 ctagtagcaa ttagtttagt tggtactttc attttgagtt ttataaataa gcaaactgaa
    23581 agattattga gtaaagaatt atatataatg cttgctttat ttataatgtt ttcatcattg
    23641 ttaggtactt gttttaattt ttatgatata aattattatg atgatttttt gcatttatgg
    23701 tcaggtataa tatcttgtat tgtggctttt tctataatta aatactttta tacacaaaga
    23761 gagataagta aaatgagtaa agtgttttta gttatatttt tatttatgtt tagtatggga
    23821 gtagcgagtt tatgggaaat aggtgaattt ttaatggata cttttgttgg gactactaca
    23881 caggccggag ggttagaaga tactatgatt gatatggtag atgctttaat aggcacaatt
    23941 ataactattc cttttatagt aaaaaaatct aatatatagt tttaaatttt ttatattaat
    24001 tataatggat ttaattaatc atgttgagtt tcaaggtcta tatgattaat taaatccatt
    24061 tttgttattt ataattatta ttgaatatat ttttacatat aagatataag gtgatagata
    24121 gaaattgttg gattatgtaa ataattaata tataatacca ttatgggcaa taaatacaaa
    24181 tcaaatgata atatagtcta tttatgtaaa tgttatattg tatcctaagt ataatagtaa
    24241 gttttttgat agtggtgtag aaagtaaaaa tctactagcc taaattgatg tatggattag
    24301 tttaggaaca gtaattgcat atcttaagtt tgatgtcatg ttgggctact agaaataggt
    24361 catctttttc acttgaatat cttttcttta aacatgggga gtatcatatc aaaaacatat
    24421 gaaaattaag tgatttatga gaacagtaat attttatatg atagtataga atagtaataa
    24481 agtattagga ggaacattga gtgttaaaaa agtggtttgg tattgtgaaa aaaagacaaa
    24541 aaagtgagtc agtaaaagaa gaaggtgaag taatattaaa aaacgaaaaa atattatctg
    24601 aagaaaagtt gatagatgaa gaaggagttg tagttaatat tgataatcaa atattaacta
    24661 aagtagtaaa tgatgacaat gaaatagaag aaaaaataat agaagaagat ttgttaatta
    24721 gtgaaaatac cataaaatta gatgataaag aagcaattat taatgataaa aacatagaat
    24781 tatgtaaaaa agaagttcaa gttgaaagtg aaaaaatagg tttaaataag tttgaaggac
    24841 tggaccaaaa tgaaaattat aatctagaaa aaaatgttat tgaagaaaaa aaagtaagtg
    24901 aatgtttgac agaagaagat ttagagtaca taaaagaaat taaaataaaa agaggaaaaa
    24961 gtataaaagc tataaacttg tatactaaag aagagtgggt ttttgacact catatacagt
    25021 gtagtaaaaa actcaaagtt ccattagggt acataagaga aaatttaaaa tatggatata
    25081 tggattactt tggagatgca ataaattatt taagtgaagt attaaatata gatgaatact
    25141 gcaaaagtga atggagctat ctcgataata gcaaatctcc atctgagata tttaatattc
    25201 taaacaataa aatatttagt ataaggcttt ctaatgaaaa aagaaatgaa attttgacaa
    25261 atgataaaat tgaagcatta aaaatgaatt atagatttga atgtattgat gaagaatatg
    25321 atgaatattt taaaaaatat aagtctataa tcaaaagagg tggaaagaaa aaagttgaat
    25381 tagtaaataa aaaaggtgac attttagaaa tatttaagtc tttagaagaa tgtgccattt
    25441 atttgcaaaa ggaaaagaat gaagttatac aaatgttaaa atatggagat acaaaagtag
    25501 gaagaaactt tataaggtat agtttgagaa gtatttaatt tcattttaaa ttcataagag
    25561 tttcatttct gattcacaca tttaatatta ttttaatgta aaataataaa ggtgaagtat
    25621 tgcacaatta aaattaagga gggtattaat gtctgaagaa aaaaatccaa aagaaaatac
    25681 attagatgaa tctaaaaatt cagataatgt aaattctaaa aaagatgatg tgtcagatga
    25741 aaaaaatact gataacaaag ttaaaaaaag ggaaataaag ctaaacaaca aaacacttaa
    25801 agctattgta gcaattgttg cagtaggagc tatatgttta ggtggaggct atgtttttgg
    25861 caaagaagct ggaagaaaat tgcctgctac ttcaaaacac tataatagca gtgaggtagt
    25921 agctactatt ggggataaaa aaataacagg tgaaatgtta agaaagagaa tggagccatt
    25981 attttattta aatgcaaaaa ctactatgac tgatgacgaa atacgttcat atgaatcaaa
    26041 tatgattgaa actatgatta catctgatat attatcctct gaagcagcaa aagagaaaat
    26101 aagtataact aatgaccaac tagatgctca gtataataat ttaatgagta gtatagaatc
    26161 agcaatggga atgactaaag aagagttttt aaaacagttt gatttgactg aaaaatatat
    26221 aaaagatgat atgaaaaaag agctagtagc atcaaaatat ttaaatgaaa aatcaaaagt
    26281 tactgataaa gaagcagaga actattacaa taaaaacaag agcaattatt tacaagttag
    26341 agcttcacat attttaataa aaactgttga tgataaaggt aagcaagtta gtagtagcaa
    26401 aaaagctgaa cttaagaaag aagctgaaga aatacttaaa aaagcccaag ctggggaaga
    26461 ttttgctact ctagctaaaa aatactctga agatagtagt gcagaatcag gtggagattt
    26521 aggtttcttt ggtaaaggtc agatggtgga atcttttgaa aaagcagcct ttgcattgaa
    26581 gaagggagag gtatctaaca aacttgtaga aagtgattat ggatatcata taattaagaa
    26641 aactgatgaa aaatatcagc catttgaaga ggtaaaatca gatttagtaa gcagtttaac
    26701 atcagaaaaa caaaacctac ttatacaaaa tctaaaagaa aaatataatg ttaaaattac
    26761 agataaatat caaatgaaag ctaaagaata gataaagtca atttattaaa aaaaagaaaa
    26821 attaactgta tggataaaag ctatttttac tctcaatata gagagtataa aatagctttt
    26881 attattttgc aataa
//
LOCUS       DAMJYR010000043        26172 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.043, whole genome shotgun sequence.
ACCESSION   DAMJYR010000043 DAMJYR010000000
VERSION     DAMJYR010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 26172)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26172)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26172
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.043"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(156..1442)
                     /locus_tag="PC471_001805"
     CDS             complement(156..1442)
                     /locus_tag="PC471_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDF2788936.1"
                     /translation="MIVGIIGPSDSGFKIKDDLKQIDSDLKTKIYVREKVVDTIEVIS
                     KCEDECDAIIFTGCAVYEFIKSKYEISKPHSFVPRSGTSIMKAFWAIKSANIKLDKFS
                     IDVVDRYVVEDALKEFDIKATSVFCNPFSLEVGESELVEWHIKLFEENKTDIMLTGFG
                     AVYNELKERGYPVFRLQATIQLIKESYDKVKSEYALSKARFSQIAVEILSLIDYKEKI
                     DNYYSDMIKKSDMDKLVVNYVRSIQGSLFLLGRNDYVVFVHKGVADNEYNYDKLFNLK
                     REIKDMGFSLSIGIGTGVTAYQAENNAHKALRHSIDSKEIGIYLVDEDENIRGPLASE
                     NELNYSLMLSDEKVIEISKKTGMSCESVAKIIAINENRKSKIYDSKELAEYLNVSERS
                     ARRILNKIVNAGLGRICAKETSIGGGRPKNITEILF"
     gene            complement(1565..4663)
                     /locus_tag="PC471_001806"
     CDS             complement(1565..4663)
                     /locus_tag="PC471_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HDF2788937.1"
                     /translation="MDVINLIDELDEIVYVSSLDTYELLYINEIGKKAMGLNDISHLK
                     CYKALQGKDSPCEFCTNSILKEDEYYIWENTNTRTNRHFILKDKLIKWEGKHARLEIA
                     LDITEKEDISKSIAEKLEIKELLVGCIKHLITESNFSDAVNLVLTNIGKFHKADRAYV
                     FEVSEDRKKVTNTYEWCNEGVDEQKQALTNLDINELKNWFLLFEQKGFVIIDDIENIK
                     EEQPIEYEILKPQKIESLMTSSLKQDDKVVGFIGVDNPHKAVGDTSLLTSLSYFLLTE
                     MKKRKVENKLNYMSYYDFLTGAYNRNKYIKYIDNFQKGKVKSIGVVFVDINGLKDIND
                     NFGHANGDSAIVSACVIIKKYFHSYNVYRIGGDEFVVLCEDISKQLFEELVSWLSEEF
                     KDEMIYSVAVGSIWSDDNIDIHKLIKVADERMYQDKKRYYNKNKLRNNEMKVISDLAD
                     STNNILFKHKELVDKVYFDDLMIKGYSKAYEKLAYNTYDIILSICIDTNLITPIYQSE
                     SNIFSFPIKENLEKTFSYIKKHLIHPDDIILFNDNHLLKSLDSLNNSKQDDLELNFEY
                     RRLGIDKKYYWALIYMLKISSKNKLGEKQTHILVAIKNIDYLMQLHKNQKDTLTGLYN
                     YESFCEQISLMLKRYPDKRYAIIMMDIDKFKVINDIYGVRGGDKALIYVAELIQKNIG
                     SNGICCRMYADVFCIFYESYSDRDIHKVIYKIKNSLNRKKFEYMLVPCFGVYRILDNH
                     IPITNACEMAGYAHKKVKKQSINNYIFYDDTIRNDALEEKQMEREMEYALENGQFHVY
                     LQPKYNLNANKIVGAEALVRWVHPEKGIIPPIKFISLFEKNGFIIRLDMYVWEQVCSL
                     IRKWIDLGEEPVPISVNVSRLHLHNPHFKEIVLSLINKYSIPKSFLELELTESLFIKN
                     IKLFTKIIADLRMDGIVLNMDDFGSAYSSLNMLKNINIDTLKIDCGFFNEEGITDREK
                     IVLKHTIAMAKDLDMDVVAEGVETKEQAEFLVTLDCVVIQGYYYCKPIPSADFEKLLY
                     QK"
     gene            5389..5547
                     /locus_tag="PC471_001807"
     CDS             5389..5547
                     /locus_tag="PC471_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2788938.1"
                     /translation="MKIFSFLLFVIGICLCIAGFIGHYPVFVGTTLSFLAIAMIAVRQ
                     VKTKKKMK"
     gene            complement(5834..6538)
                     /locus_tag="PC471_001808"
     CDS             complement(5834..6538)
                     /locus_tag="PC471_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2788939.1"
                     /translation="MEIKPLVLIVEDDKPICKFIKVSLETQNYRCVETDNGGTAISLI
                     HSLDPDLIILDLGLPDIDGIEVIGRVRACAKTNKIIVVSAREHERDKVEALDGGADDY
                     LTKPFSVTELLARVRVALRNKAQQDNINNDAPKSFEVKNLKIDYENHIVSINGEEIHL
                     TPIEYKIIELMSKYSGRVLTHKFIIDKVWGNYYESENQSLRVFMASIRRKIEKNPAQP
                     EYILTEVGVGYRMADE"
     gene            complement(6529..8136)
                     /locus_tag="PC471_001809"
     CDS             complement(6529..8136)
                     /locus_tag="PC471_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4118 domain-containing protein"
                     /protein_id="HDF2788940.1"
                     /translation="MKKERLQHEDLKEYDYDEDGYLSKKIIIKDFIKTILVMTIATGI
                     SVVFEKVGFNESNIILVYILGVLFATSITYGYFFGILSSVIGVLSFNFFFTHPLYTFL
                     AYRKDYPMTFCIMLMAAIITSTLTSRIKREVRLSHKREKMIRLLYENNKTLLKAKNKN
                     QIIEFCGSNLVNILNRTVIVTIADENNNLGKPSIYICNNDGSENIFHSVLEKEAIKEA
                     FRTGNPVGIGTKYCKNSSAYYFPIKGQESNVLGVIGVACFKIDIITENEKILLEAVST
                     QVALAIDRENLFEKNKKANLEAERERLRGNLLRSISHDLRTPLTSILGSSSTILENDD
                     VIDKETRVELLKNIYEDTSWLTHSVENILSMTRIDEGKLDIEKRPEVVDEIIAESILR
                     VKKFANSRDIKTNIPDEIIIVHVDVLLIEQVLVNLIDNAIRHTPKNSKIELTVKKESN
                     QVLFEVADNGKGIPNEDIGNIFNRFYTKNKSRNLERRGIGLGLEICKSIVEAHGGEIV
                     AFNNPSGGATFRFSIPMYDEEGKDKWK"
     gene            complement(8390..9568)
                     /locus_tag="PC471_001810"
     CDS             complement(8390..9568)
                     /locus_tag="PC471_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2788941.1"
                     /translation="MEKLSDKRRVKAVSMETFVFIVLLAVGFGYVGSIMGAGMMFKVI
                     MSTAHALLLDTVFLIMAMAVLAGALSALLSEFGVISLVNKIFKGLMRPIWGLPGASIA
                     GVVATYLSDNPAIIPFAKDKTFTQYFKKYQVPALCNLGTAFGMGLIVTTFMIAQGKEY
                     VLPAIIGNVAAIIGSIISVRIMLTFTKKYYNYDPKNDTEKQINDKGAKLEEFREIRDG
                     NVFQRTLDAILEGGKLGVEMGMAIIPGVLVVCTLVMLLTFGPSTDPATGQAVYTGAAY
                     EGIKLLPAIGDKISFIIEPLFGFTSPEAIAFPVTALGAVGAAISLVPEFIKSGAITPN
                     DIAVFTAMGMCWSGYLSTHIGMMDALDARPLAGKAILSHTIGGLCAGICAHFIFMLVG
                     "
     gene            complement(9794..10288)
                     /locus_tag="PC471_001811"
     CDS             complement(9794..10288)
                     /locus_tag="PC471_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HDF2788942.1"
                     /translation="MHLFIEVIAAFFTALSFGVLFNMKGKNLILAGIGGSIAWFSYKF
                     LLNIGVTENLCFFIATVCFAIYCELCARIYMTPATTLSVCCLIILVPGYGIYNTMYSV
                     LTNNYIKAVEYGVSTLSCASSIALGLVFITTVFRKVNLYGVLTKIKENEKYKKSINKI
                     KQQK"
     gene            complement(10289..11050)
                     /locus_tag="PC471_001812"
     CDS             complement(10289..11050)
                     /locus_tag="PC471_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HDF2788943.1"
                     /translation="MDIDRILNFSSNAGKAMLQSGGETYRVEETIARICQSFGIDHVD
                     VFATPTAVMASVFVDGKLHSAIKRISSRRVDLNLVHEVNSLSRAISINNLDIELCEKI
                     LDEISEDNYYSDLITIFFAGIAAATFSILFGGNVEEFIAAFTVGILTKFVIMYLSKSS
                     LNDFFVNAIGAVIIASCSILILKLGFIKTLDHLIAGAIMLLVPGLALTNALRDLLDGQ
                     LISGLAKLAEVFFVGVSIAVGMFLVLGLYFKLGGI"
     gene            11752..14586
                     /locus_tag="PC471_001813"
     CDS             11752..14586
                     /locus_tag="PC471_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDF2788944.1"
                     /translation="MLRKDKVLLSLHKLCENMTLKDLQSDKTGFTTIEIANHSSLDRS
                     NTSKELNNLYNEKKVLKISGKPILFLHASIIENLIGRTLNSDEFSINNCNELLKSSKN
                     DSEDIFSTLLGHDSSLKLPIEKAKSAILYPPKGLNTLLIGPTGVGKSTFAETMYKYAV
                     ESKIFSSESKFVVFNCAEYAENANLLLSNLFGYVKGSFTGANKDKFGIVAQADGGILF
                     LDEIHRLPPEGQEMLFLLMDKGIYRRLGESDIMHKANVFIVCATTEDIHSSLLGTFLR
                     RIPVTINLPALQDRSLKERLSLIKYFFIIESKYTNATIKVHKDVIKSLLLYDCFGNIG
                     QLKSDIQLMSARAFLNYKTGLKDNIEIDLSLVPDYIYIGLLKLNENRSNLNKLSELDY
                     MLFYEFNGNNLEVEYTKELSKYNIDTSNNGFKYSTEIPYLENDIRSMFINYMEKYSTK
                     LLSISSVTHSDYTSNEIFKVINPKIYYAVEHSLKEAEKISRNKYTKQTYIALSMHISA
                     LIENINQDTYNPKYNLYNDKIFLDKDLNIAKIVLSIIENDLQTSLPKDEVKFISMFLN
                     SMYLEPNNQSESIGVIILAHGNSTASSMCDVANSLLGTNHCNAIDMPLDVKVSTILDK
                     TIELVKECDEGKGVLLLTDMGSLIAFGELISEKTKIKTRSIEMVSTPIVLEAVRKSTL
                     PEMTLDELANSLSSLNPYIGRAAIDSTEINYPEKSREYVIITTCITGYGSAMKVADFI
                     NSSLKNISKYNINLVPHNIESFKMYKNKFSNTKILAVVGSVDFNVANTPFIHIEELLN
                     GYGLDMLNKIIEGTFNANSITNIKETNLSYNSLIINFMTQNLELLDSVKLFNYVNSSL
                     FTIIELIDENLKSKFKLGYILHCSFMIERCFKNTTFPYNNITELISKNHELYSLIREA
                     FKPTEAYFNILIPDEEIAYIIDMINEYLNK"
     gene            14629..15873
                     /locus_tag="PC471_001814"
     CDS             14629..15873
                     /locus_tag="PC471_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HDF2788945.1"
                     /translation="MEDEILKGKIKQLTILALIFIFITPVFAFADTPPVPNSSRAALL
                     IDQETKRILFEKNIDEKMPLASLSKMMTFLLAIEAVDKNQVKETDMVKIDKSTASVGG
                     STCKLKDGDEISLGELMQGLMLVSGNDAAIAIAKHIGKTEKNFVNMMNKKAEEIGMID
                     TYYFNPNGLPIYTDPEHKEPPIENMSTAHDIVTLGKYMYDHYENQVTRITTMQVYNDT
                     KKDFTHYNTNPLLVSVPGVDGIKTGYTDNAGYCLAFSMMVPKDAKNERNHRLIGVVLG
                     DGNKKNRISSSATLLKYGKDNFHSKKIAHKGDIIETPCVDGIDDFKITVKVDKDLYGV
                     VSDNENINPKVVFKNMNYPIHKGDIVGVAKYYNDSGKFVGSVDVKSESNIGCIPLKDK
                     IKIKVAKINKKLEIKNSVCFKA"
     gene            complement(16288..18030)
                     /locus_tag="PC471_001815"
     CDS             complement(16288..18030)
                     /locus_tag="PC471_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2788946.1"
                     /translation="MKKTINNNLFRILNIIVYKNKTKLIVSFIMVFIIAGVNIVIPQL
                     IRCIIDDGIGNKNIFLLINLILIYIFIRILSATFDVILSYLYTSIKNKVTINLKVKLL
                     NHISKLSGRYYSSKKTGNILSIMESDIFMIENFGAEIIFDIIVNVFTAIISLIFLINL
                     QFDLFLVVLFLQLILTFSQNKMTKLISKKISEIRSDSGSLSNLIQEYVSNIMDIVILN
                     SKKNFFNKYLKRERDLIKKDLNLNIIMSSNIAISQILTGLITILIYGIGGYKIINNKM
                     TMGELIAFQQYTGMLIGPCIAIVRANTRIAQSKVSINRVFSVIDEKIDIKVDNNGYRI
                     DNSEIKKIDFKNVSFRYGEDLNLVLNDINLQFEKGEVTGIVGGSGCGKSTITNLIYRL
                     WDINEGDILIDGVNIKDINLKSLRNNIDIVTQDQFVFDDTIKNNIVLNNKISDKELDE
                     ILEVTCLDDFIKNLDKGLDEVVGEKGVKISGGQKQRISIARALVSNKKVLILDEATSA
                     LDNISQKEILNNLKEYIQDKIVIIIAHRLSTIKSADNIYVLDKGRVIESGNDAELLHK
                     DGLYNKLVETADFA"
     gene            complement(18014..18688)
                     /locus_tag="PC471_001816"
     CDS             complement(18014..18688)
                     /locus_tag="PC471_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018590550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2788947.1"
                     /translation="MKILKVKDLKKIYGKYENEVRALDGIDLDIEPHKFTAIVGASGS
                     GKSTLLHCVAGLDKPTLGNVYLDDVDLYTLNDDKLSEIRRQEFGFIFQSYNLIPVINV
                     YDNIVLPVLLDGKKEDKEYVMRLINKLGIEKQIKKYPNELSGGQQQRVAIARALANRP
                     SVIFADEPTGNLDSKTTNEVMNMLEWCVKEYKQTLVMITHNDNIAKTADRIITIKDGK
                     IDEKNY"
     gene            complement(18725..19714)
                     /locus_tag="PC471_001817"
     CDS             complement(18725..19714)
                     /locus_tag="PC471_001817"
                     /inference="COORDINATES: protein motif:HMM:NF012311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HDF2788948.1"
                     /translation="MTKVRIAVLDTGLDVNDDDINGNYVYDSNFQLKDLGGKEINDIN
                     GHGTFVSKTILNISKNTQIYPINIFNDSGKTSSLNLLSTLSKLTMSDIQIINISAASI
                     NSMYEKELEKVCNILNSQGRFIICSKHNKYEDKESIPTIFESVIGVVGNENIYKNNQY
                     IYEPENKIQMYANSKDNFIKFKDKVTHFGKNSKACAVATGIVANILKNNPYIKFNELE
                     EILIQKSKSKEDLKIENLNTPSYKSNLIRLDIARKIINVINRNFSKIKIDFEFVSEYS
                     LFNNFTCIGNYNAYNFLTILNKEFNISIDYKEVYLYQLENLDMAVDLIYENLI"
     gene            complement(19762..21006)
                     /locus_tag="PC471_001818"
     CDS             complement(19762..21006)
                     /locus_tag="PC471_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HDF2788949.1"
                     /translation="MNSSNKYFLDCICEKIINNNQVILSNKVNGRWVKIPIECYEVLK
                     YSVDNALQLGNLYDVFQENEDKKYYERVIDKLEHIGLLSDSILSEFRLERINKISFSP
                     TNRCNLSCDYCCVDSKIDNNDYLDTSQVRKVIDNIVKLNPRILTITGGEPLMRNDFLE
                     LLDYIKEKFKGKIILSTNATLIKEKQIDDIINGLYAIEISLDGYDELSCSEVRGKGVF
                     CKVLNVIRLIKLKKFENVGLSMVVGKNNEKDIEKFNKLNEDLGTTPIIRNFMNIGRGN
                     ENYSKYLNDKLDIDYICKDDYVDESSLCSNMCKAGVNQISIDHKGDVYPCPNLEYKDL
                     KIFNILEFNENILERILNREMDIFNIFDKLKPVNIESCKECKVNLFCTTCPSKIYTLK
                     NNKKMFDANCKKMKNILEPILW"
     gene            complement(21048..23594)
                     /locus_tag="PC471_001819"
     CDS             complement(21048..23594)
                     /locus_tag="PC471_001819"
                     /inference="COORDINATES: protein motif:HMM:NF014717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2788950.1"
                     /translation="MINIKLAVSYLKRQKGKTLSLILSIGIAVMLVFSLSVINESQGQ
                     SDINHAYKNSGDYHAFYEDINSDIVKELNGEEDIKEFNDVLKFGEIISKDNGASLNLC
                     SYNKDFLDSTRYKLVKGREPRTSNELVIEQKVLDKMELDVKLNQTIDFQIVNEHINKN
                     NENSIYSKDKQFKIVGILSKSDEYYENLNKYKVRAFTYSNNGKDIVPYELITHMGTLK
                     LNTKDLDSQKVVNLGRKYKLTSNQFYPNTEVSMSMYMKNISKKTTYSIKQSLLPLLCA
                     TLVIYNMFNIIILDMTKQIGLLRAVGASKRNIRQVFFIQSIVVLSVGIVIGLLGGIVF
                     SYLGLMIVYDKSAELTINTNSIIESIIMSIIAVILASVVPIYKAGNLSIIDAIKKTDK
                     LNYIPRLLLLKSHKKVVGIINEIAFKNIFRNKVTSIIIIITISLCGVLFIGRLTSTKF
                     IYGKESDSANITSKSYGNFDIYLGYSPINANYTFSKYDNSLLNEIKAIEDVTDVEPNI
                     YLKGYLRNADLEEDYLDELKRTEINNNNESTVLIRGYNKELLNNIDKYIDKGENVYSY
                     TDRDYKNVLLVNNFYSRIKLSNNTQIIKSPKIGDLIDIKIPVYKDGEYKYINTKVRIA
                     GIMKNSYISEQDGESQSGGIQIIFNEKDLKELTGISDYNKVFVKIKKETDKKVIKEIN
                     NIIEKSGFSDIEGRYVRNHFGDHYNKSSYKLAMICVFGVLLISSINIIFIVRSNIIVR
                     LSEICTFRAIGMSKKNVKRMLIKENMIYGVLSMIVAGIISSFNYYKIVSETNKLNQQV
                     YGINNSIKFEIPIYEILIFGSISVFVCVIAVYFSNKMINKLSIVSGIKEN"
     gene            complement(23802..23963)
                     /locus_tag="PC471_001820"
     CDS             complement(23802..23963)
                     /locus_tag="PC471_001820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2788951.1"
                     /translation="MIHIKKIEEKIELQDVEVQADPCKWYYDKHGIISQIDCMVDCAG
                     STPKGTVAY"
     gene            complement(24019..25035)
                     /locus_tag="PC471_001821"
     CDS             complement(24019..25035)
                     /locus_tag="PC471_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HDF2788952.1"
                     /translation="MKFTIFVTEDCNLRCTYCYEGNDKKKENMSYETADKTIDFICNK
                     ELESNSDYTTIVLHGGEPFINFDLIKYIHRDLVDRLSDKKILFDITTNGTIINDEIIE
                     FIAKNVKNISISIDGKKNIHDINRIDANGVGTYEKVMENGKRLIDKGIYVRARMTYNS
                     NTVKYLYESVYDIAKQGFKNIAPISDFMDKNWDNSHIKILNEQISKIMNIKKDYPDID
                     ISILDKQFINKKKGDCFGGINSYAIDTRGDVYPCTVCVGDKDILLGNIYDGLDDKKIK
                     NLIKIYISKNEECDGCTRLDYCMSTRCKLINKKVTGKFQSAVPIYCGLERVYVETTKK
                     LCSL"
     gene            complement(25032..>26172)
                     /locus_tag="PC471_001822"
     CDS             complement(25032..>26172)
                     /locus_tag="PC471_001822"
                     /inference="COORDINATES: protein
                     motif:HMM:NF015983.1,HMM:TIGR04085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HDF2788953.1"
                     /translation="IYDFVNYFDDESDKKYIINLIDIMNKNQIFKSNNLNFDMLSNGV
                     AIYFCLTNRCNLFCKHCCVESSSNKKDLLSTYDLKNIVDIISVLNTPHIIITGGEPLI
                     REDFFEIIKYIKQKLPDTKLILSTNGTLIDEHNVDFIITNFEKIDISIDGVDENTCSE
                     TRGKGVFNKVISSVNLLKDNGFSNIKLSMVFGQKNSHLNKQFKDLNKKLGTEFVERYF
                     IPTGRGLENKESYLIEDTVLPITIPYILDNELKMCKDKKNKKMGCCACNAGNSQIFIN
                     YDGDVYPCPSLIKDDYKMGNIFDNEFVKVIKENKTRDLKSLIKLESIYPYNFEKCKDC
                     DVNIFCWHCPAILDSVKDNELELDRWCSMMKHNLNTAVWGDEKVN"
BASE COUNT         8976 a         4292 c         2679 g        10225 t
ORIGIN      
        1 ataataggct ctagtatttt atattaataa tttattctag tattttacat aattatttgt
       61 tctaattttt ttcataataa gctttaatac tttatatagt aatttgttgt agaattttat
      121 acaatataat gatttataca gttttataaa atatcttaaa acagtatttc agtaatattt
      181 tttggtcttc ctccaccaat actagtctct tttgcacata ttcgacctaa acctgcattt
      241 actattttat ttaatatacg tcttgcactt ctttcactta catttagata ctctgctaac
      301 tcttttgaat cataaatctt actttttcta ttctcattta tagcaataat ttttgcaact
      361 gactcacaac tcattccagt ctttttagat atttcgatta ccttttcatc agatagcatc
      421 aaagaataat ttaattcatt ttcggaagct aaaggtcctc ttatgttttc atcttcatct
      481 accaaatata taccaatttc ttttgaatct atggagtgtc ttaatgcttt atgtgcatta
      541 ttttcagcct gatacgcagt tactccagta ccaattccta tactaagaga aaagcccatg
      601 tcttttatct ctctctttag attgaatagc ttatcataat tatactcatt atcagctacc
      661 cctttatgaa caaatacaac ataatcattt cttccaagta aaaataagga accttgaatg
      721 cttcttacat agtttactac aagcttatcc atatctgatt tttttatcat gtcagaataa
      781 tagttatcta tcttctcttt ataatctatt aaactcaata tttcaacagc aatttgggaa
      841 aatcttgcct tacttagagc atattcactc tttaccttgt cataactttc tttgattaat
      901 tgtatagtag cttgtaaacg aaatacagga tagcctcttt cttttaattc attatatact
      961 gcaccaaaac cagtcaacat tatatccgtc ttattttctt caaataattt tatatgccat
     1021 tctacaagtt cagactctcc tacttccaaa gaaaatggat tacaaaatac actcgttgct
     1081 ttaatatcaa attctttaag tgcatcttcc acaacatatc tatcaactac atctatactg
     1141 aatttatcta attttatatt tgcacttttt attgcccaaa atgccttcat tatactcgtc
     1201 ccacttctcg gtacaaatga atgtggctta cttatctcat acttagattt tataaattca
     1261 taaactgcac atcctgtaaa tataatagca tcacattcgt cttcacattt agaaataact
     1321 tctattgtat ctactacttt ttctcttaca tatattttag tctttaaatc actgtctatc
     1381 tgttttaaat catcctttat cttaaaccca gagtctgaag gacctataat acccactatc
     1441 ataaacttcg ctcctaatcc ataaatatta tactttttaa aaattatatc ctcactattt
     1501 tctacttcta tttaattatt ttgaactact ccatcctacc tacatttaga atggaataat
     1561 tagcttattt ttgataaagt aacttctcaa aatcagcact tggaataggc ttacaataat
     1621 aataaccttg aattaccaca caatctaaag tcactaaaaa ttcagcttgt tcttttgtct
     1681 ctacaccttc tgccactaca tccatatcta aatcttttgc catggctata gtgtgcttta
     1741 aaactatttt ttctctgtct gtgattcctt cctcattaaa gaaaccacaa tctatcttta
     1801 atgtatcaat attaatattt tttaacatat ttaatgaaga atatgctgaa ccaaagtcat
     1861 ccatatttaa aacaatacca tccattctaa ggtcggctat tatcttagta aataatttta
     1921 tatttttaat aaataaactt tctgtaagct ctaattctaa aaaagatttt ggtatactat
     1981 atttatttat taaacttaaa acaatttctt taaaatgtgg attatgcaaa tgtaatcttg
     2041 atacatttac agagatagga actggctctt cacctaaatc tatccatttt ctaatcaaag
     2101 aacatacttg ttcccataca tacatatcta atctgataat aaacccattc ttctcaaata
     2161 gtgaaataaa tttaattgga ggtattatac ctttttctgg atgaacccat ctaaccaatg
     2221 cttctgcacc aacaatttta tttgcattaa gattatactt aggttgtaaa tatacatgga
     2281 attgcccatt ttcaagtgcg tattccattt ccctttccat ctgtttttct tcaagtgcat
     2341 catttcttat agtatcatca taaaatatat agttattgat actttgtttc tttacttttt
     2401 tgtgagcata tcctgccatt tcacacgcat ttgtaattgg aatatgatta tccaaaattc
     2461 tataaacccc aaaacatggt actaacatat attcaaattt ttttctattt aagctatttt
     2521 taatcttata aattacctta tgaatatctc tatcagagta actctcataa aaaatgcaaa
     2581 atacatctgc atacatacga caacatatac cattagaacc aatgtttttt tgaatcaact
     2641 ctgcaacata tattaaagct ttatctcctc ctctaactcc ataaatgtca ttaattacct
     2701 tgaacttatc tatatccatc attataattg cataacgctt gtcaggatat cttttaagca
     2761 ttaaacttat ttgttcacaa aaactttcat aattgtatag tcctgttaat gtatcttttt
     2821 ggtttttatg tagttgcata agataatcta tattttttat agcaactaaa atatgtgttt
     2881 gtttctcacc taacttattt ttactactaa tctttaacat ataaattaat gcccaatagt
     2941 actttttatc tattcctaaa cgtctatact caaaatttag ttctaaatcg tcttgcttgc
     3001 tattattaag actatcaaga gattttagaa ggtggttatc attaaataat attatatcat
     3061 caggatgaat taaatgtttt tttatgtatg aaaaagtttt ttcaagattt tcctttatag
     3121 gaaaactaaa aatattgctc tctgattgat aaattggagt aattaaattc gtatcaatgc
     3181 aaatagataa aattatatca tatgtattat aagctaattt ttcataagcc ttagaatacc
     3241 cttttatcat taaatcatca aaataaacct tatcaactaa ctctttatgt ttaaataaaa
     3301 tattattcgt cgaatcagct aagtcactaa ttactttcat ttcattattt ctaagtttgt
     3361 tcttattata atacctcttc ttatcttggt acattctctc atcagcaact ttaattagct
     3421 tatgtatatc aatattatcg tcactccaaa tactgcccac tgctacacta tatatcatct
     3481 catccttaaa ttcttcactt aaccaactca ctaattcttc aaatagttgt tttgatatat
     3541 cttcacataa tacaacaaac tcatctcctc caatacggta aacattataa gaatgaaagt
     3601 atttttttat tattacacat gctgacacaa tagcagaatc gccattagca tgtccaaagt
     3661 tatcattaat atcttttaac ccatttatat ctacaaatac aactcctatg gatttaactt
     3721 ttcctttttg aaaattatct atatacttaa tatatttatt tctattatat gcaccagtca
     3781 agaagtcata gtagctcata taatttaatt tattttctac ttttcttttt ttcatctcag
     3841 ttaataaaaa ataagatagg gatgtcaaaa gagaggtatc tccaacagct ttatgtggat
     3901 tatcaactcc aataaaccct actactttat catcttgttt tagtgaagat gtcattaaac
     3961 tttcaatttt ttgaggtttt aaaatttcat attctattgg ctgttcttct tttatatttt
     4021 ctatatcatc aattattaca aaaccttttt gttcgaataa taaaaaccaa ttctttaatt
     4081 cattaatatc aagatttgtt agtgcctgtt tttgttcatc aacaccttca ttgcaccatt
     4141 catatgtatt agttactttt tttctatctt ctgatacttc aaaaacatat gctctatcag
     4201 ctttatgaaa tttaccaata ttggttaata caagatttac agcatcagaa aaattagatt
     4261 ctgttataag atgtttaata catccaacta ataactcctt tatctctaat ttttctgcta
     4321 ttgacttact tatatcttcc ttttctgtaa tatcaagagc tatttctaat cgtgcatgtt
     4381 tgccctccca tttaattaac ttgtccttta atataaaatg tctatttgtt cgagtattcg
     4441 tattttccca tatgtaatat tcatcttctt ttaaaataga gtttgtacaa aattcgcagg
     4501 gagaatcttt cccctgtaat gctttataac attttaaatg ggatatatca ttaagtccca
     4561 ttgccttttt cccaatttca ttaatatata aaagttcata tgtatctaaa gaagaaacat
     4621 atacaatttc atccaactca tctattaaat ttattacatc catttatata tatctccttt
     4681 tattcatttt ccagattgaa tttttaaagc ttttaaaatt ttgctatata tttggcaatg
     4741 atattatatt tctttatgtt ttaaaatagt accttaatga ggttctattt tttagtaaat
     4801 agaattatta ttgtgtcaat cgtacaatag agttttgtaa tacctaattt aatatattta
     4861 taattaatta tctcattatg tgtttataat taattctcat ataaatgaca aatctccacc
     4921 tcaattaagt attccaatca tatatgaaga taatacttca ttcatttaaa aatataaaac
     4981 tattatatat taaaatttct tcaataataa attatctaga ttgttacttt gatatattat
     5041 ataatatcac aaaggctgat ataaaaagac aaaaagggca atttttttat atcaaaaaaa
     5101 aatgttaaat tgatattatc catatataaa ttgatattat ccatatagat gctatattta
     5161 acaattctac ttacttccct tacacttatt agcatatagt tattaagagt ttgttgcaaa
     5221 acaacactta gcacacttta aaaagtgtgg tctaataagt aatcaacgaa gaagacataa
     5281 taaagcataa aatatgatat acttttccac gccatcaaaa ctgaggattt tattattaat
     5341 atatgctaaa atatgcttaa taaattaaat tttaatggga gaatagttat gaaaatattt
     5401 agtttcttgt tgtttgtgat aggaatatgt ttatgtattg ctggatttat aggacattac
     5461 ccagtatttg ttggtacaac attgagtttt cttgcaattg caatgatagc tgtaagacaa
     5521 gtcaaaacta agaaaaaaat gaaataggca aatcctaact ttacaagtta atattacaca
     5581 agtaattagt ctaaaattgg atactttttc tattacagaa atacatcatc cactttgtat
     5641 tttcttaaaa aaataaaata gctatgtaaa ttcaagatta gatagattaa ctctctatcc
     5701 atttcattgt aaaatataca tagctatttg tattcgattt aaaatactta gattttctta
     5761 cataacaaat aatttctatc tgattattac tcatattcat ttagtaacca ctcagcaact
     5821 aatcatttag tatctattca tccgccatac ggtaacctac cccaacctca gtcaaaatat
     5881 attctggctg agcaggattc ttttcaattt tccttcttat acttgccata aaaactctaa
     5941 gcgactgatt ttcactttca tagtaatttc cccaaacttt atctattata aacttatggg
     6001 ttaagactct tcctgaatac ttagacatca actcaataat cttatattcg attggagtta
     6061 aatgtatttc ttcaccatta attgacacaa tatgattttc ataatctatc tttaggtttt
     6121 taacttcaaa agactttgga gcatcattat ttatattatc ctgttgagct ttatttctaa
     6181 gagccacacg tactctagcc aaaagctctg ttacactaaa aggtttagtt aaataatcgt
     6241 ctgctcctcc atctaaagct tccaccttat ccctctcatg ctctctcgct gaaacaacaa
     6301 ttattttatt agtctttgca catgccctaa ctcgtcctat tacctcaatc ccatcaatat
     6361 ctggtagtcc taaatccaaa attatcaaat ctgggtcaag agaatgtata agtgatatag
     6421 ctgttcctcc attatctgtc tctacacacc tataattttg agtttcaaga gatactttta
     6481 taaatttaca aattggctta tcatcttcta caattagaac taatggcttt atttccactt
     6541 gtctttcccc tcctcatcat acataggtat gctaaatcta aatgtagctc ctccagaagg
     6601 attattaaag gcgacaattt ctccaccatg agcttctact atagatttac atatctccaa
     6661 tccaagacca atacctcttc tttcaagatt tctagactta ttcttagtat aaaacctatt
     6721 aaaaatattc ccgatatctt catttggtat tccttttcca ttatctgcaa cttcaaatag
     6781 tacttgattt gactcttttt ttacagttag ttctatctta gaatttttag gtgtatgcct
     6841 tatagcatta tcaattagat ttactaacac ttgttctatt aataatacat ccacatgaac
     6901 gattattatc tcatctggaa tatttgtttt tatgtctcta ctattggcaa actttttgac
     6961 tcttaatata gattcagcta ttatttcatc aacaacttct ggtcgttttt caatatccaa
     7021 ttttccttca tctattctgg tcatactaag tatattctca acagaatgag ttagccaact
     7081 cgtatcttca tatatatttt tcaataattc tactctagtt tctttatcta tcacatcatc
     7141 attttcaagt attgttgaag atgaacctaa tatacttgta agtggagttc ttaaatcatg
     7201 agaaattgac ctaagcaagt ttcctcttaa tctctctctc tctgcctcaa gatttgcttt
     7261 tttatttttc tcaaataaat tttctctatc aattgccaac gcaacctgtg ttgaaactgc
     7321 ttctaatagt atcttttcat tttctgtaat aatatctatc ttaaaacacg caacacctat
     7381 aacaccaaga acatttgatt cttgaccttt tatagggaaa taatatgcac tgctgttttt
     7441 acaatatttt gtcccaattc caactgggtt acctgtccta aaagcttctt ttatagcttc
     7501 tttttctaat actgaatgga atatattctc acttccatca ttgttacaaa tataaatact
     7561 tggcttacct agattattat tctcatctgc aatagtcact attacagttc tatttaatat
     7621 atttactaaa ttagacccac aaaattcaat aatttgattt ttattttttg ctttaagcaa
     7681 cgttttgtta ttctcataga gcaatcttat cattttttct ctcttatgag aaagtctcac
     7741 ttctcttttt atcctagaag ttaaagtact tgttataatt gctgccatta gcataataca
     7801 aaaggtcata ggataatctt ttctataagc taaaaaagta tataatggat gcgtaaagaa
     7861 aaaattaaat gatagcactc ctattactga agaaagtatt ccaaaaaaat atccataggt
     7921 tatacttgta gcaaatagta cacctaatat gtatactagt ataatattag attcattaaa
     7981 acctaccttt tcaaaaacta ctgatattcc agtagcaatt gtcataacca atatagtttt
     8041 tatgaagtcc ttaataatta tctttttact gagatagcca tcttcatcat aatcatactc
     8101 ttttaagtct tcatgttgta gtcgttcctt tttcatttac tacaattcct ccttattgtt
     8161 actgtttaaa ttggctctat tttatataat acaattttat cataataaat tcaatcaata
     8221 catttaatta atgtacttaa attactaaaa ggtgctgtct taaaatgaaa ataaatctct
     8281 atttcaaaac agcacctata tatttaaatt actaatttag tttttacata cttaaatcaa
     8341 tagttttatt caatttcata aattataata cttcaattat aaaacatttc tatccaacaa
     8401 gcataaatat aaagtgggca catatacctg cacataaacc acctatagta tgcgaaagta
     8461 tcgctttacc agccaatggt cttgcatcta aagcatccat catacctatg tgtgtactta
     8521 aatatccact ccaacacata cccattgctg tgaatactgc tatatcatta ggagttatag
     8581 cgcctgattt tataaactct ggtactaaag atattgctgc tccaacagct cctaatgcag
     8641 ttacagggaa tgctattgcc tctggtgatg tgaatccaaa taatggttct attatgaagc
     8701 taatcttatc accaatagca ggtaataatt ttataccttc atatgcagct cctgtgtaaa
     8761 cagcttgacc tgtagcaggg tctgtagatg gtccaaaagt taataacata actaatgtac
     8821 aaactactaa aactcctggt attatagcca tacccatttc aacacctaac ttaccacctt
     8881 caagtatagc atccaatgtt ctttggaata cattaccatc tcttatttct ctaaactctt
     8941 caagttttgc acctttatca tttatttgct tttcagtatc atttttaggg tcataattat
     9001 aatacttttt agtaaaagtc aacataattc taacactaat aatactacct attattgccg
     9061 caacattacc tattatagct ggtagtacat attctttacc ttgagctatc ataaaagttg
     9121 ttacaataag tcccatacca aaggctgttc ctaaattaca taaagcaggt acttgatact
     9181 tcttaaaata ttgtgtaaat gttttgtctt tagcaaatgg tataatagct ggattatcag
     9241 ataaatatgt tgcaactact cctgctatac tagcaccagg taagccccat ataggtctca
     9301 taagaccctt aaatatctta ttaactaaag atattactcc aaactctgaa agcaatgcac
     9361 taagtgcacc tgcaagaact gccatagcca ttattaaaaa tactgtgtct aataaaagcg
     9421 catgtgctgt actcattatt actttaaaca tcattcctgc acccatgatg cttcctacat
     9481 atccaaatcc taccgccaat agtacaataa atacgaaggt ttccatactt actgccttga
     9541 ctcttctctt atccgacagc ttttccatct tgaaatcttc ccccttttaa ttttaaatta
     9601 aggttcctaa cattggtaca aattttttgg tacaaactga gttctaaaac ttacactcgt
     9661 aatgcaagtt gtcagttttt tgaaataaaa aaaaattacg tttatagaat aagaatatcc
     9721 tctttaattt ggatactctt attctatttt atgttattat attatcatcc ttttgtttaa
     9781 aagtcaattt tttctatttt tgttgtttaa ttttatttat actcttttta tatttttcat
     9841 tttcttttat ctttgtaagt actccatata agttgacttt tctaaataca gtagttataa
     9901 ataccaatcc taatgcaatt gaacttgcac acgataatgt actcacccca tattctacag
     9961 cttttatata attattggtc aaaacagaat acatagtatt atatattcca taacctggta
    10021 ctaatattat taaacagcaa acacttaatg tagttgctgg agtcatataa attcttgcac
    10081 ataactcaca atatatcgca aaacaaactg tagctataaa aaaacataag ttttctgtta
    10141 ctcctatatt taaaagaaat ttatatgaaa accaagctat acttccacct attcctgcta
    10201 gtattaagtt ttttcctttc atattaaata gcactccaaa gcttaaggca gtaaaaaaag
    10261 ctgctattac ttctataaac aaatgcactt atatcccccc taacttgaag tacaatccaa
    10321 gtaccaagaa cattcctact gctatagata cacctacaaa aaatacttct gctaattttg
    10381 caagaccaga aatcaactgt ccatctaaca aatctcttaa tgcattagtc aaagcaagcc
    10441 caggaactaa gagcattatt gctcctgcta ttaaatggtc taacgtcttt atgaatccga
    10501 gctttaaaat taaaattgaa caagaagcta taataactgc tccaattgca tttacaaaaa
    10561 agtcattaag ggaagattta cttaaataca taataacaaa cttagtcaga atcccaacag
    10621 taaatgcagc tataaattct tccacatttc ctccaaataa tatcgaaaaa gttgctgctg
    10681 caatacctgc aaaaaatatt gttattaaat ctgagtaata gttatcttca cttatttcat
    10741 ctaaaatttt ctcacataat tcaatatcta aattatttat acttattgct cttgataatg
    10801 agttaacttc atgaaccaag tttaggtcta ctcttcttga gcttattctt ttaattgctg
    10861 aatgtaattt cccatctaca aatacagatg ccattacagc tgttggagtt gcaaatacat
    10921 ctacgtggtc tattccaaaa gattgacata ttctagctat cgtttcttca actctatagg
    10981 tttctcctcc actttgcaac atagctttac ctgcatttga ggaaaagttt aaaattctat
    11041 ctatatccac aaaacatatc tcccattctt ttatagtatt attcatattt taatacaaga
    11101 tattatttat ttaaactatt ttaaataatt catatccaat atttacattt tatgaaaaaa
    11161 cttgtttttt atgcttgtca aaatcaaatt ttaagcttgt ttacaccata aaatggctga
    11221 attaatattt gacaaacact ttcaaccatt tatataacct tctaaattgt ataacttttt
    11281 actacaaata aaattatatc tcttttaatt ctttctcttg agtatttagc ttatagtaac
    11341 ttcatattta tatatgtaca atataaaaca ttatttttat gatacaaaat ataatttgta
    11401 aatgtatata tgtattaact gtgtatatat aaattattct tgaccttatc agttttttca
    11461 tatatcttca tctataccaa ccatatatta ataaatgata ttacatagac ttaagtatat
    11521 taaaaaactt aaatttagtt atttaacaac ataatactct aatttaatct ataaaaatcc
    11581 atacttaata taatgcaact cttattttaa caccatctat tacacaaaac aacatattaa
    11641 aaagtaaaat acaaaatatc cactataaac cttattttaa tactgaatat atattataat
    11701 atatttaatg tagcttaaat atagtttaaa cataaagaaa ggaaacggta tatgctaaga
    11761 aaagataaag ttctattatc tctacataaa ttatgtgaaa atatgacttt aaaagacctt
    11821 caatcagata aaacaggatt cactacaata gaaattgcta accattcatc tcttgataga
    11881 agtaatacca gtaaagaatt aaataaccta tacaatgaaa aaaaagttct gaaaatatct
    11941 ggtaagccaa ttttatttct acatgctagt attatagaaa atttaattgg ccgtactttg
    12001 aacagtgatg aattttctat aaataattgt aatgagctac ttaaatcaag taaaaatgac
    12061 tcagaagata ttttttcaac attacttgga catgattcta gtcttaaact acctatagaa
    12121 aaagctaaat ctgctatact ctatcctcca aaaggtctta atacgttact aataggacct
    12181 actggtgttg gaaaaagtac atttgctgaa acaatgtaca aatatgctgt agaaagtaaa
    12241 atcttttctt ctgaaagcaa attcgtagta tttaactgtg ctgaatatgc agaaaatgct
    12301 aacttattat taagtaattt atttggttat gtcaaaggtt cttttactgg tgctaataag
    12361 gataaattcg gcatagttgc acaggctgat ggaggcatat tattcttaga tgaaatacac
    12421 agattgccac cagaaggtca agagatgctt tttcttttga tggacaaagg catatacaga
    12481 cgattaggag aatctgacat tatgcacaaa gctaatgtct ttattgtgtg tgccacaaca
    12541 gaagatatac attcttctct gcttgggact tttttaagaa gaatccctgt tacaatcaac
    12601 ctacctgctt tacaggatag gtctttaaaa gaaagattat ctcttattaa atacttcttt
    12661 attatagaat caaaatacac caatgcaact ataaaagttc acaaagatgt aattaaatca
    12721 ttactccttt atgattgttt tggtaatata ggtcagctta aatctgacat acaacttatg
    12781 agtgcaagag ccttcttaaa ttacaaaact ggacttaagg ataatataga aatagattta
    12841 tcccttgttc ctgattacat ctatattggt cttttaaaat tgaatgaaaa tcgtagtaat
    12901 ctaaataaat tatcagaact tgattatatg cttttttatg aatttaatgg taataactta
    12961 gaagttgaat atacaaaaga actatctaaa tacaatatag atacatcaaa caatggattt
    13021 aaatactcta cagagattcc ttatctggaa aatgatataa ggagtatgtt tataaattac
    13081 atggaaaagt actctacaaa attactttcg atatcttcag taactcattc tgattataca
    13141 agtaatgaga tttttaaagt aatcaatcca aagatttact atgctgttga acattcttta
    13201 aaagaggctg aaaaaatatc gagaaacaaa tacacaaaac aaacttatat tgctctttca
    13261 atgcatataa gtgcacttat agaaaatata aatcaagaca catataatcc taagtacaat
    13321 ttatataatg ataaaatttt cttagataaa gatttaaata tagctaaaat agttttaagt
    13381 ataattgaaa atgacttaca aacatcttta cctaaagatg aagtaaaatt tatatccatg
    13441 tttctaaata gcatgtatct tgaaccaaac aatcaatctg aaagtatagg agtcataata
    13501 cttgctcatg gaaattcaac agcaagcagt atgtgtgatg tagcaaattc attgcttggt
    13561 acaaaccatt gtaatgcaat tgacatgcct cttgatgtaa aagtaagtac catacttgat
    13621 aaaactatag agttggtaaa agaatgtgac gaaggtaagg gtgtacttct tttaacagat
    13681 atgggctctc ttatagcttt tggagaactt atttcagaaa aaacaaaaat caaaactcgc
    13741 tcaattgaaa tggtatctac tccaatagtt ttagaagcag taagaaaaag tacactacca
    13801 gaaatgactt tggatgaatt agctaattcc ttaagctctt taaatccata tattggacga
    13861 gcagcaatcg attcaacaga aataaactat cctgaaaagt caagagagta tgtaataata
    13921 actacttgta taacaggata tggttcagct atgaaagtag cagactttat aaattcttca
    13981 cttaaaaata ttagtaaata caatattaat ttagttcctc ataatataga aagttttaaa
    14041 atgtataaga ataaatttag taatactaaa attctggcag ttgttggaag tgtagatttt
    14101 aatgtagcca acaccccttt tatacacata gaggaactac taaatggata tggcctagat
    14161 atgctaaaca aaataataga aggtacattc aatgccaata gtattacaaa tataaaagaa
    14221 acaaatctat catacaacag tttaataata aactttatga cccaaaactt agagcttttg
    14281 gactctgtta aattattcaa ctatgtcaat tcttccttat ttacaataat agaattaata
    14341 gatgaaaact taaagagcaa atttaagcta ggatatatac tacattgctc ttttatgata
    14401 gaaagatgtt ttaaaaatac aacttttcca tataataaca ttactgaatt aattagtaaa
    14461 aatcatgaac tatatagttt aatacgtgaa gcctttaagc caacagaagc ttattttaat
    14521 attttgatac cagacgaaga aattgcttat ataatagaca tgattaatga gtatctaaat
    14581 aaataaaatt tatagcactt attttgtgca tattaattaa tatatgaaat ggaggacgag
    14641 attttgaaag gaaaaataaa acagttaaca atacttgcat taatttttat atttataact
    14701 cctgtatttg catttgcaga tactccacca gtaccaaatt cttcaagagc agcactactt
    14761 atagaccagg aaactaaaag aatattattt gaaaaaaaca ttgatgaaaa aatgccatta
    14821 gcgagtttaa gtaaaatgat gaccttttta ttggcaatag aagcagttga taaaaatcaa
    14881 gttaaagaga ctgatatggt gaaaatagac aagtctacag cttcagtagg aggttctact
    14941 tgtaaactta aagatggtga tgaaatatct ctaggagagc ttatgcaggg tctaatgcta
    15001 gtatctggaa atgatgctgc tatagccata gctaaacata ttggtaaaac tgaaaagaat
    15061 tttgtaaata tgatgaacaa aaaagcagaa gaaataggaa tgattgatac gtattatttt
    15121 aatccaaatg ggcttcctat atatactgac ccagaacata aagaacctcc tattgaaaat
    15181 atgtcaactg ctcacgatat agttacactt ggaaagtata tgtacgacca ttatgaaaat
    15241 caagttacaa gaataaccac tatgcaagta tataatgata ctaaaaagga ttttacacat
    15301 tataacacaa atcctctgct tgtttcagta cctggtgttg atggaataaa gacaggatat
    15361 acagataatg ctggatattg ccttgcattt tctatgatgg tacctaaaga tgctaaaaac
    15421 gaaagaaatc atagattgat aggtgttgta cttggtgatg gtaacaagaa aaatagaatt
    15481 tcttcttccg ctactttatt gaaatatggt aaagataatt tccattctaa aaagatagca
    15541 cataagggtg atattataga gactccttgt gttgatggta ttgatgattt taagataaca
    15601 gttaaggttg ataaagattt gtatggtgtt gtttcagata acgaaaatat caatcctaag
    15661 gtagtgttta aaaatatgaa ttatcctatc cataagggag atattgttgg tgttgcaaag
    15721 tattataatg actctggtaa atttgttgga agtgttgatg taaaaagtga aagtaatata
    15781 ggatgtattc ctttgaagga taagattaag attaaggttg ctaagataaa taaaaaactt
    15841 gagattaaaa attctgtttg ttttaaagca tagtaaagca tattattaaa agggtatgtt
    15901 aaatttataa agactccact ttgtggagtc tttttgtttt ttacaaaaaa ttaaaatagt
    15961 ggcaacaatt gaggatgtga gggagagttg ccactatttt gtatagggta ttatattatg
    16021 tttttaagct agttttacta tcttctatgt ttatataata actcttgtgt gtgaatttaa
    16081 ttgataatta atatgaaaat tatgtgaagt tttgatttat ttgtaatata ttctactact
    16141 attataattt ttctactgga tatactaatc atttgtttaa cattatctga gattgatttg
    16201 caaactttta tataactaaa ttagttatat aacttataga ttccattaaa taccctaata
    16261 atcatatatg tacatacaaa gtcatgctta tgcaaaatct gcagtttcaa ctaacttatt
    16321 atataaacca tctttatgta gaagttcagc atcatttcca gattctatta ctctaccttt
    16381 atctaataca tatatattat cagcactctt aattgttgaa agtctatgag ctattataat
    16441 aactatctta tcttgtatgt actctttcaa gttgttcaat atctcttttt gtgaaatatt
    16501 atctagagct gaagttgctt cgtccagaat aagtaccttt ttattactta ctaaagctct
    16561 agctattgat atacgttgtt tttgacctcc tgatattttg acaccttttt ctcctactac
    16621 ttcgtccaat cccttatcta gattttttat aaaatcatct aaacaagtaa cttctaatat
    16681 ctcatctaat tctttatcac ttattttatt atttaatact atattatttt ttattgtgtc
    16741 atcaaataca aattgatctt gagttactat atcaatatta tttcttagtg attttaaatt
    16801 aatatccttt atattaacac catctattaa tatatcacct tcatttatat cccaaagtcg
    16861 gtatattaga tttgttattg ttgatttgcc acatccactt ccaccaacaa ttcctgttac
    16921 ttcccctttc tcaaattgta aatttatatc atttaaaaca aggtttaaat cctcaccata
    16981 tctaaaagat acatttttaa aatctatttt cttaatttca ctgttatcta ttctatatcc
    17041 attattatca actttaatat ctatcttttc atctattact gaaaatactc tattgataga
    17101 cacttttgat tgtgctattc tagtattcgc ccttactata gctatacatg gtccaattaa
    17161 cattcctgta tattgttgaa atgctattaa ctcccccatc gtcatcttat tatttattat
    17221 tttatatccc ccaattccat atattaatat tgttatcaat cctgttagta tttgagatat
    17281 tgctatatta ctactcataa tgatgttcaa attcaaatct ttttttatca aatctctctc
    17341 tctctttaaa tacttattaa agaaattctt ttttgaattt aatataacaa tatccataat
    17401 attagatacg tactcttgaa tcaaatttga taaactgcct gaatcacttc ttatttcaga
    17461 aattttctta gatataagtt ttgtcatttt attttgagaa aatgttaata tcaactgtaa
    17521 aaataaaact actaaaaata aatcaaactg caaattaatt aagaaaatta acgatattat
    17581 agctgtaaac acatttacaa taatatcaaa tataatctct gctccaaagt tttctatcat
    17641 aaatatatca ctttccataa tggataaaat attacctgtt ttcttacttg aataatatct
    17701 accagaaagt tttgatatat ggttaagcaa ctttactttt agatttatag taactttatt
    17761 ttttattgaa gtatataaat atgataatat aacatcaaat gtagctgata atatccttat
    17821 aaaaatatat atcaatatta aatttataag cagaaaaata tttttatttc ctattccatc
    17881 atctattata catctaatta gttgaggtat tacaatattt acgccagcaa ttataaaaac
    17941 cattataaat gatactatca acttagtctt attcttatat actattatat ttagtattct
    18001 aaataaatta ttattaatag tttttttcat ctatttttcc atcttttata gttataattc
    18061 tatcagctgt ctttgcaata ttatcattat gagttatcat aactagtgtt tgtttgtact
    18121 ctttaacaca ccattcaagc atattcataa cttcatttgt agtcttacta tctaagttgc
    18181 cagtaggttc atctgcaaat attactgacg gtctattggc taaagctcta gctattgcaa
    18241 ctctttgttg ttgacctcct gatagttcat tcggatattt ctttatttgt ttttcaatcc
    18301 ccaacttatt aattaatctc ataacatatt ctttatcttc ttttttccca tcaagcaaca
    18361 caggaagtac tatattatca tatacattta taactggtat taaattgtaa ctttgaaata
    18421 tgaagccaaa ttcctgtctt cttatttcag acagcttatc atcatttaat gtatataaat
    18481 ctacatcatc taagtacaca ttccctaaag ttggcttgtc taagcctgca acacagtgaa
    18541 gtagtgtact tttaccagaa ccacttgcac ctacaatagc tgtaaattta tgtggctcta
    18601 tatctaaatc aataccatca agagctctaa cttcattctc atattttcca tatatttttt
    18661 ttaaatcttt taccttaagt attttcataa cgtatctcca ttcccattta aaaaacatat
    18721 atttttaaat taaattttcg taaataagat ctactgccat atctaaattc tctaattgat
    18781 ataagtatac ttctttgtag tcaatactaa tattaaactc tttatttaaa atagttaaaa
    18841 aattatatgc attataattt ccaatacatg taaaattgtt aaataaagaa tattcactta
    18901 caaattcaaa gtctattttt attttagaaa agtttctatt tatcacattt attattttcc
    18961 ttgctatatc caatcttatc aaattagatt tataacttgg agtgttcaag ttttctattt
    19021 ttaaatcttc tttacttttg cttttttgaa taagtatttc ttccaattca ttaaacttaa
    19081 tatatggatt gttttttaat atatttgcaa caatgccagt tgccactgca caagccttac
    19141 tatttttacc aaaatgagtt actttatctt taaatttaat aaaattatct tttgaatttg
    19201 catacatttg aatcttattt tctggttcgt atatatattg attattttta tatatatttt
    19261 cattacctac tacaccaata acactttcaa atatagtagg aatactttct ttatcttcat
    19321 atttattatg ttttgaacaa ataataaatc taccttgact atttaaaata ttacaaacct
    19381 tttctaattc tttttcatac atactattaa tagatgcagc tgaaatgttt attatctgta
    19441 tatccgacat agtcaacttg ctcagtgtac taagaagatt taaagaactt gtttttccag
    19501 aatcattaaa tatattaata ggataaattt gggtattttt acttatattt aatatggttt
    19561 tcgatacaaa agttccatgt ccatttatgt catttatttc cttccccccc aaatctttca
    19621 attgaaaatt tgaatcataa acataatttc cattaatatc atcatcattt acatctaatc
    19681 ctgtgtctaa gactgctatt cttactttag tcaattaata atcacctcta aaataaatta
    19741 ccttgaatat aaaactaatc cttaccataa tataggttcc aagatgtttt tcattttttt
    19801 acaatttgca tcaaacattt ttttgttatt ttttaaagta tatattttcg atggacaagt
    19861 tgtacaaaat aaatttactt tacattcttt acaactctca atatttactg gttttaattt
    19921 atcaaatata ttaaatatat ccatttctct attaagaatc ctctctaata tgttttcatt
    19981 aaattctaat atattgaata tctttaaatc cttatactct aagtttggac acggatatac
    20041 atctccttta tgatctatac ttatctgatt aacacctgct ttacacatat ttgaacataa
    20101 actactttca tccacataat catctttgca aatgtaatca atatcaagtt tatcattaag
    20161 atatttgcta tagttctcat tacctctacc aatattcata aaatttctta ttataggcgt
    20221 tgtacctaaa tcttcattta atttattgaa tttctctatg tctttctcat tattctttcc
    20281 taccaccata gacaaaccta cgttctcgaa tttttttagt tttataagtc taatcacatt
    20341 taatacttta cagaatactc ctttacctct tacttcacta caacttaatt catcataacc
    20401 atctaaacta atttctattg catataaacc attaattata tcatcaatct gtttttcttt
    20461 aattaaagtt gcatttgtag acagaattat tttaccttta aatttttctt ttatataatc
    20521 caacagctct aagaaatcat ttctcatcaa tggctctcca cctgtaatgg ttaatatcct
    20581 tggatttaat ttaactatat tatctattac ttttcttact tgagatgtat ctaaataatc
    20641 gttattatct attttagaat ctacacagca ataatcacaa cttagattac atctattagt
    20701 tggagaaaat gaaattttgt ttattctctc tagtctaaat tcacttagaa tagaatcact
    20761 tagaagtcct atatgctcta acttatctat aactcgttca taatattttt tatcttcatt
    20821 ttcttggaat acatcataca aattaccaag ttgtaatgca ttatctactg aatactttag
    20881 aacttcataa cattctattg gaattttaac ccatcttcca tttactttat tactaagaat
    20941 aacttgatta ttatttatta ttttttcaca tatacaatct aaaaaatatt tgtttgagct
    21001 attcatttat tttatttcct tcctatatat ttaaattaga aatttcatta attttctttt
    21061 ataccactta ctatactaag tttatttatc attttattag aaaaataaac tgcaatgaca
    21121 caaacaaaaa cagatataga tccaaatatt agaatttcat atattggaat ttcaaattta
    21181 atagagttat ttataccata tacctgttga ttaagtttgt tagtttcaga aactatttta
    21241 taataattga aactagatat aattccagct acaatcatac ttaatacccc atatatcata
    21301 ttttctttta ttaacattct ttttacattt tttttggaca tccctattgc tctaaatgta
    21361 catatctcac tcaacctcac aattatattt gatctaacta taaaaataat gtttatagat
    21421 gatattaata aaactccaaa tacacaaatc attgctaatt tatatgagga tttattataa
    21481 tggtctccaa aatgattacg tacatatctt ccctctatat ctgaaaatcc acttttttct
    21541 attatattat ttatttcttt tattactttt ttatctgttt ctttttttat cttcacaaaa
    21601 actttattat aatcacttat ccctgttaat tcttttaaat ccttttcatt aaatataatt
    21661 tgaattccac ctgattgaga ttctccatct tgctctgata tatagctatt tttcattatt
    21721 ccagcgattc ttacttttgt atttatatat ttatactctc catctttata tacaggaatt
    21781 tttatatcta ttaagtctcc tattttaggt gattttataa tctgagtatt atttgacaat
    21841 ttaattctag aataaaaatt atttaccaat aaaacatttt tataatccct gtctgtataa
    21901 ctataaacat tttcgccttt atctatatat ttatctatat tatttaatag ctctttattg
    21961 taacctcgaa ttaaaactgt agactcatta ttattattta tttcagttct ttttagctca
    22021 tctaaataat cttcttctaa gtcagcattt cttagatatc cttttaaata aatattgggt
    22081 tcaacatctg ttacatcttc aatagctttt atttcattta ataaagaatt atcatattta
    22141 gaaaatgtat aatttgcatt tattggtgaa tatcccaaat aaatatcaaa attgccatat
    22201 gatttacttg ttatattagc tgaatctgac tcttttccat atatgaattt tgtagaagtt
    22261 agtctgccta taaataaaac cccacatagt gatatcgtaa ttattattat tattgatgta
    22321 accttattcc taaatatatt tttaaaagct atctcattta taattccaac aactttttta
    22381 tgtgatttta acaacaaaag cctaggaata taatttaact tatctgtctt ttttatagca
    22441 tcaattatag ataaatttcc agctttatat attggtacta cagaggctaa tataactgct
    22501 ataattgaca tgatgataga ttcaattata gagtttgtat ttatagttaa ttctgcactt
    22561 ttatcatata ctatcatgag acctaagtaa gaaaaaacta tacctccaag taaaccaatt
    22621 actataccta cagataaaac tacaatactc tgaataaaaa atacctgtct aatatttctc
    22681 ttgctagctc caacagctct aagtaaacca atttgcttgg tcatatctag tattattatg
    22741 ttaaacatat tgtatattac taatgttgca cataataaag ggagtaagct ttgctttatt
    22801 gaataagttg ttttttttga aatatttttc atatacatag acatacttac ttctgtattt
    22861 ggataaaatt gattacttgt taacttatat tttctcccta gattcactac tttttgagaa
    22921 tctaaatctt ttgtattcaa ttttaatgta cccatatgag ttattaattc atatggaaca
    22981 atatctttgc cattgtttga atatgtaaaa gctcttacct tatatttatt taaattttca
    23041 tagtattcat cagatttact taaaatacca actattttaa attgcttatc tttactataa
    23101 atagaatttt cattattttt atttatatgc tcattaacaa tctgaaaatc aatagtttga
    23161 tttaatttga catctaattc cattttgtct aaaacttttt gttctattac taattcatta
    23221 gaagttctag gctctctacc tttaactaac ttatatctag tagaatctaa aaaatcttta
    23281 ttgtaagagc ataaatttag agatgctcca ttatctttac ttataatttc tccaaattta
    23341 agtacatcat tgaattcttt tatatcttct tcaccattaa gttcttttac tatatcacta
    23401 tttatatctt cataaaaagc atgataatct cccgagtttt tgtatgcatg atttatatct
    23461 gattgtcctt gagattcatt aataacactt aatgaaaaaa ctagcataac agcaatccct
    23521 atactaagta ttaatgataa agtttttcct ttttgtctct ttaaatatga caccgcaagt
    23581 ttaatattta tcataatcag aatcctcctt attcatattt acaagaaaaa ctctataacc
    23641 taaaatagta ggttatagag cactattcat taaatactaa ctaactcgtt gtgctagtta
    23701 aatttaatta ttaaaaatac tagagttgag aaaatcacat ttcatttgta aaaaactaca
    23761 tctagtactt tcaacatatt taatagactg attagtattt tttaataagc tactgttcct
    23821 tttggagttg atcctgcaca atccaccata cagtctattt gtgaaattat tccatgctta
    23881 tcataatacc atttacatgg atctgcttgc acctcaacat cttgaagttc tattttttct
    23941 tcgatttttt ttatatgtat cataatacat atcccccttt acttttgttt tatatttatt
    24001 ataactagtt aaactagatt ataaactaca tagttttttt gttgtttcca cataaactct
    24061 ttctaatcca caataaattg gtactgcact ttggaattta cctgttacct ttttattgat
    24121 aagtttacat ctggttgaca tacaataatc aagtctagta catccatcac actcttcatt
    24181 cttagatata taaattttta taaggttctt aattttttta tcatctaatc catcatatat
    24241 atttcctagt aaaatatctt tatctcctac acatactgta catggatata catctcctct
    24301 agtatctatt gcataactat ttatccctcc aaaacaatct ccttttttct tatttataaa
    24361 ttgtttatct aaaatagata tatctatatc aggataatcc tttttaatat tcataatttt
    24421 actaatttgt tcatttaata ttttaatatg agaattatcc caatttttat ccataaaatc
    24481 tgaaattggt gcgatatttt taaacccttg ttttgctata tcatatacac tctcataaag
    24541 atacttaaca gtatttgagt tatatgtcat tcttgcccta acatatatac ctttatctat
    24601 aagtcttttt ccattttcca ttactttttc ataagtacct actccgttag catctattct
    24661 atttatatca tgtatattct tttttccatc aatacttatc gatatgtttt ttacattctt
    24721 tgctataaac tctattattt catcatttat aatagttcca tttgtagtta tatcaaataa
    24781 tatctttttg tcactcaatc tatctactaa atccctatgt atatatttaa tcaaatcaaa
    24841 atttataaat ggttctcctc catgtaaaac aattgttgta taatcactat tactttctaa
    24901 ttctttatta caaataaaat ctattgtttt atcagcagtt tcataactca tattttcttt
    24961 ttttttatca ttaccttcat aacaatatgt acatctcaaa ttgcagtctt cagttacaaa
    25021 tatagtaaat ttcaattaac tttttcatct ccccatacag ctgtatttaa attgtgtttc
    25081 atcatactac accatctatc caattccaat tcattatcct taactgaatc taatatcgct
    25141 gggcaatgcc aacaaaaaat atttacatca caatctttac atttttcaaa gttatatgga
    25201 taaatacttt ctaatttaat caaagacttt agatctcttg ttttattttc ttttataact
    25261 tttacaaatt cattgtcaaa tatatttccc attttatagt catcctttat cagtgaagga
    25321 caaggatata catctccatc ataatttata aatatttgag agtttcctgc attacatgca
    25381 caacatccca tttttttatt ttttttatct ttacacattt ttaattcatt atctaaaata
    25441 tacggaattg taattggaag aactgtatct tcaattaaat agctttcttt attttctagg
    25501 cctcttcctg ttggaataaa atatctttca acaaattctg ttccaagctt tttatttaaa
    25561 tctttaaatt gtttatttaa atgagaattt ttttgtccaa ataccataga cagctttata
    25621 ttactgaatc cattgtcttt tagcagatta acagagctta ttactttatt aaatacacct
    25681 tttcctcgtg tttcactaca agtattttca tctactccat caatactaat atctattttt
    25741 tcaaaattag ttataataaa atcaacatta tgttcatcta ttaaagttcc atttgtggat
    25801 aatattaatt tggtatcagg taatttttgt tttatatatt taattatttc aaaaaaatct
    25861 tctcttatca atggttctcc accagttata attatatgag gagtatttaa aactgatatt
    25921 atatctacga tattttttaa atcatatgta ctaagtaaat ctttcttatt agaagaagat
    25981 tcaacacaac aatgtttaca aaataaattg catctatttg tcaaacaaaa ataaattgca
    26041 acaccattgc ttaacatatc aaagtttaaa ttgttacttt taaaaatttg atttttgttc
    26101 ataatatcta taagattaat aatatatttt ttgtcacttt catcatcaaa ataatttaca
    26161 aaatcataaa tt
//
LOCUS       DAMJYR010000044        26095 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.044, whole genome shotgun sequence.
ACCESSION   DAMJYR010000044 DAMJYR010000000
VERSION     DAMJYR010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 26095)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26095)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26095
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.044"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(91..234)
                     /gene="scfA"
                     /locus_tag="PC471_001823"
     CDS             complement(91..234)
                     /gene="scfA"
                     /locus_tag="PC471_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="six-cysteine ranthipeptide SCIFF"
                     /protein_id="HDF2788954.1"
                     /translation="MENKHVKTLSQATLKQSAAKGGCGECQTSCQSACKTSCTVANQE
                     CER"
     gene            complement(300..662)
                     /locus_tag="PC471_001824"
     CDS             complement(300..662)
                     /locus_tag="PC471_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04086 family membrane protein"
                     /protein_id="HDF2788955.1"
                     /translation="MGKSNYIFKGLGYAYIITLAVLLVYNLFLTFTDIGGDNITMVSS
                     FITTISAAIGGFYTSKHMKEKGLMYGLLVGLLYIVCIFLTVFLAQEKFVFEVGMIYKL
                     LLISAAGGIGGVLGVNFK"
     gene            complement(720..1013)
                     /gene="yajC"
                     /locus_tag="PC471_001825"
     CDS             complement(720..1013)
                     /gene="yajC"
                     /locus_tag="PC471_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HDF2788956.1"
                     /translation="MPQQQIIMSIGLWVVVIAIFYFLMIRPQKKKDKQLKEMRSSLSV
                     GDKVITIGGIVANVAKVEDDVVILELGPNRTKVPFEKWAIGTVKSKKEEIEED"
     gene            complement(1147..2268)
                     /gene="tgt"
                     /locus_tag="PC471_001826"
     CDS             complement(1147..2268)
                     /gene="tgt"
                     /locus_tag="PC471_001826"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HDF2788957.1"
                     /translation="MYAVRYELIKTCKQSGARLGRLHTPHGIIETPIFMPVGTQATVK
                     SMTPEELKEIGSQIILSNTYHLYMRPGHELIKRAGGLHKFMNWDKPILTDSGGFQVFS
                     LGPLRKIKEEGVEFRSHLDGSKHFLTPEKAMEIQNALGSDIMMAFDECAPYPSDREYV
                     KNSLERTTRWLKRCKDAHNNTDKQALFGIIQGGMYKDLREQSAKEITNIDLPGYAIGG
                     LSVGEPKPLMYDVLEHTTPLMPKDKPRYLMGVGSPDDLVEGVIRGVDMFDCVLPTRIA
                     RNGTAMTSQGKVVVRNATYAEDFTPLDPECDCYACKNYSRAYIRHLIKANEILGARLI
                     TTHNLHFLLNLMKQIRQAIMEDRLLDFRNEFFAKYGYEI"
     gene            complement(2347..2832)
                     /locus_tag="PC471_001827"
     CDS             complement(2347..2832)
                     /locus_tag="PC471_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2788958.1"
                     /translation="MHSLQKQKKNNPILKIAFIFERVLAVVVLIAVFLGTIDVLRLMW
                     GAYIVDFQNPVQYSQLNDFLAQILLLVIGVELVVMLSLHIPGAFIEALLYAIARKMLL
                     LPKNEGMIDVLIGVIAIAGLFAIKKFLVTKDKSALNIMSIHDEEAIKEQQNKEDEDKS
                     I"
     gene            complement(2844..3869)
                     /gene="queA"
                     /locus_tag="PC471_001828"
     CDS             complement(2844..3869)
                     /gene="queA"
                     /locus_tag="PC471_001828"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HDF2788959.1"
                     /translation="MKTSDFKFDLPQELIAQVPIEDRASSRLMVLDKETGNIEHKVFR
                     DIIEYLNPGDCLVLNNTRVIPARLIGEKLETGGKIEFLLLKRTEEDTWQALVKPGKRA
                     KVGTKFSFGNGKLIGEVVDLSDEGSRIIKFHYDGIFEEILDELGNMPLPPYITARLDE
                     KERYQTVYSKHNGSAAAPTAGLHFTEELLNKIKEKGVDIAFVTLHVGLGTFRPVKVED
                     VLNHKMHSEYYMVSQEAADKINRAKENGKNVICVGTTSCRTIESACNEDGKMKETSGW
                     TEIFIYPGYKFKVLDKLITNFHLPESTLIMLVSAICGKDNVLNAYNEAVKERYRFFSF
                     GDAMIIK"
     gene            complement(4187..5206)
                     /gene="ruvB"
                     /locus_tag="PC471_001829"
     CDS             complement(4187..5206)
                     /gene="ruvB"
                     /locus_tag="PC471_001829"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HDF2788960.1"
                     /translation="MQGFEDENRIITSTMKMEDIDIENSLRPKTLEDYLGQEKSKEQL
                     SIFIEAAKSRNEQLDHVLLYGPPGLGKTTLASIIANEMGVNLRITSGPAIERAGDLAA
                     ILTNLNENDVLFIDEIHRINRSVEEVLYPAMEDFCLDIIIGKGPSARSIRLDLPKFTL
                     IGATTRAGMLTNPLRDRFGVICKLDYYTVDELSKIVLRSSSILDAEIQSNGALELAKR
                     SRGTPRIANRLLKRVRDFAQVRADGKITDKVAKDALELLGVDSLGLDFVDEKLLMTII
                     EKFRGGPVGLDTLAASIGEDRNTIEDVYEPYLLQLGFINRGPRGRVAMPLAYEHLKIP
                     YPNEK"
     gene            complement(5313..5915)
                     /gene="ruvA"
                     /locus_tag="PC471_001830"
     CDS             complement(5313..5915)
                     /gene="ruvA"
                     /locus_tag="PC471_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HDF2788961.1"
                     /translation="MYSYIKGTVEEIYIDSIVVENNGIGYKINVSSNTIMNLQVGEAT
                     KIYTKLIVREDDMSLCGFVSREELKMFELLTSVSKIGPKVALSILSFASPAQLGAYIL
                     SEDIGKLSKAPGVGKKTAERIVLELKDKVDKNNIEFEPTLLSQKPTLISQDESVDALV
                     ALGYTLSESKEAVQKCKKDGMNTEAIIKKALTYIMSKSLK"
     gene            complement(5908..6417)
                     /gene="ruvC"
                     /locus_tag="PC471_001831"
     CDS             complement(5908..6417)
                     /gene="ruvC"
                     /locus_tag="PC471_001831"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HDF2788962.1"
                     /translation="MIILGIDPGIAIVGYGIIEYKNSKFKAIDYGAVTTPAHMNISRR
                     LELVYKGIDTIVKNYNIDEVGMEELFFNKNVKTAITVAQARGVTMLACAHNGKPVYEY
                     TPLQVKQGVVGYGRADKAQVQQMVTSFLSLKKVPKPDDVADALAVAICHAHSNKLEKT
                     LKNIGGKYV"
     gene            complement(6729..7358)
                     /locus_tag="PC471_001832"
     CDS             complement(6729..7358)
                     /locus_tag="PC471_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2788963.1"
                     /translation="MFEKKKKELFPWKISALILVGVFVLSSGVYIGTRLKHNGVQTGE
                     VVSTSANMNEKSKEAFGLNKNCEVWLQSEGENETNYKRTLTMAGIVPESLLDKTKSEI
                     VSYFKKEYPTKKIKSMDKNEIILVESKYSDNKDVQAMDVTIKGKYTIENDNGNIALYK
                     YDDSGSKTLVEKTKIRVDSLPKTVQDEIKKGVVMDTEEEAYSRLEDFAS"
     gene            7561..7884
                     /locus_tag="PC471_001833"
     CDS             7561..7884
                     /locus_tag="PC471_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1540 domain-containing protein"
                     /protein_id="HDF2788964.1"
                     /translation="MERINCNVINCSHNNSHICYADKIFINGEKAHNDRHTCCSSFLD
                     ESIYSNLSSNVNNKNCEELVCTVKNCKYNKDGSLCTLDNILVAPETDRVNLYIETCCS
                     SFECK"
     gene            8152..9006
                     /locus_tag="PC471_001834"
     CDS             8152..9006
                     /locus_tag="PC471_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2788965.1"
                     /translation="MSTFYIQFLYFVIYSFIGWCCETTYCSVLQKQFVNRGFLNGPFC
                     PIYGFGALAIVATLTPFVHNIPLLFLFSIIITSIMEYCTSFILEKIFNMTWWDYSKHK
                     FNIHGRVCLENSLMFGVLSLIVMLIVHPIVVDFINSISKNVLFIFAISIEIYFVLDLV
                     ITVHTILQLNGKLKQINLIIKELKDKKEYYKLITQETIENKLESLVENRLDTLEDRFE
                     ILKEKENYRYAKNRIDELKNKLNTLLSNHKLLHRRIIKAFPNISSNKHTDILNKIKEN
                     IKSIKKDK"
     gene            complement(9192..10628)
                     /locus_tag="PC471_001835"
     CDS             complement(9192..10628)
                     /locus_tag="PC471_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HDF2788966.1"
                     /translation="MREVKPISIDILNTFKQVDEDRLNKLLADELKHLDRKIVVLDDD
                     PTGVQTVHDISVYTDWDKDSMEQGFNEKNSMFFILTNSRGFTVAQTTKAHKEISKNIV
                     DVSKKVNKDFIIISRSDSTMRGHYPLETNLLKSEVERLSGKLFQGEIIMPFFKEGGRF
                     TIDNVHYVKEGEILVPAGMTEFAKDKSFGYKSSDIGEWCEEKTNGKYKSSDMIYISSK
                     ELRELNIEAITEKLKKAEGFNKIIVNATDYVDVKVFTIAFIRAVNGGKEFMFRSAAAI
                     TKVLGGVSDKELLTKDELVSKGNTNGGIILVGSHVNKTTQQLEELKNCKYPIEFIEFN
                     QHLVLQENGLKNEVKRVIKVVEEKISSGKTVAVYTRRERFDLDTNDKDKQLMISVEIS
                     DAVTSIIGMLNVRPNFIIAKGGITSSDVGTKALRVKKATVMGQIKPGIPVWMTGKESK
                     FPNMPYIIFPGNVGEISTLRESVEILMG"
     gene            complement(10699..12081)
                     /locus_tag="PC471_001836"
     CDS             complement(10699..12081)
                     /locus_tag="PC471_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HDF2788967.1"
                     /translation="MITGYGLLIAFVISIGILLVSIIKFKVNPFLALLITSIITGFMV
                     RMPINEISTTISTGFGNTLGSIGIVIGLGIIFGNILSESRATESIAKGLLAKTGEKNS
                     ALAVTTAGFLISIPVFMDAAFVIMMPIIKYVSRVTKKSLMVFVCALGVGTIVGHALVI
                     PTPGPLAVAANVNANVGSFILYSIIVAFPAALIGGWIYGKRFEKYPAYAIDENDREKN
                     LEQGKDSIKIEDDDSSKVPGFGISMFSLLFPILLILISNVFSMFLEKGSTMSGVLAFI
                     GDKNIAILLGILVAIGFLKKYINKPMGDVVIEAADSAGLILLITGSGGAFGSVINASG
                     IGNFLVDTMSGLSISVVVLGFLLSALLRISQGSATVALVTTSSILGPTILATGMSPVL
                     VGLAICAGGVGFSLPNDSGFWVLSRFSGLSVKDTLNSWTIGGTIAGVVAFIMVLLLSV
                     INGIIPLPGL"
     gene            complement(12217..13122)
                     /gene="garR"
                     /locus_tag="PC471_001837"
     CDS             complement(12217..13122)
                     /gene="garR"
                     /locus_tag="PC471_001837"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HDF2788968.1"
                     /translation="MEGYKMKLGFIGLGIMGKPMAKNLLKDGCNLLVYDINKSAVDEL
                     ISCGAKYASVLEMGQECDIVFTILPNGTIVQDILFGMDGLAKTLKEGSIVVDMSSVTP
                     TESILCANKLKDMGLEFIDSPVSGGEPKAIDGTLAFMAGGKEEIYKKVEPFFNIMGSS
                     SILIGDNGSGSVTKLTNQVIVNLTIAAVSEAFVLAAKAGADPEKVYKAIRGGLAGSTI
                     LDVKIPMIMNRDFKPGGKISINLKDIKNVMQTAHNLDVPLPMTSQLLEIMQTLKVHGH
                     LEDDHSGIAQYFEKLAGVEIKKHDN"
     gene            13518..14189
                     /locus_tag="PC471_001838"
     CDS             13518..14189
                     /locus_tag="PC471_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDF2788969.1"
                     /translation="MATFKRLQEQAYDYLKELILSGEMIENEIYSETKLASEIGISRT
                     PIRDALQRLSQDGFIDIIPSKGFRIHQITANEIVEIFQIRSAIEGFCTFLITSQYKEA
                     RAVETISKLKHLLDKQKDILLGDKNLNSFAEYDTLFHTTIVSYAQNTEFDKMFNNYMY
                     RIKKLALDSLSHEGRLETTLKEHTDIFNNIANGCIEDIYRTTLIHMETPKYINLEDFC
                     NNKLF"
     gene            14292..14840
                     /locus_tag="PC471_001839"
     CDS             14292..14840
                     /locus_tag="PC471_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2788970.1"
                     /translation="MIFLNEDVVTNETIDKDYAIEEVRMACKHFGDLYFYFSKVLFEE
                     FGEDKTSEILRKVLFERSEERAIAMRERAHENGDELIADNIISTTDVPFLGWVPEFEE
                     LHCPYGASWLLRFEENPWFKKFASLYCDVTDTTVAEVFTGDTSHKITKNILWGDKSCE
                     RIYFHDDKVSEGKYTYGNKNCK"
     gene            15102..15410
                     /locus_tag="PC471_001840"
     CDS             15102..15410
                     /locus_tag="PC471_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2788971.1"
                     /translation="MFNKIYEKKEFIVFQVKKGYVVYNTRKSFEEGHTHLKHFEAAKT
                     AIDLAINKKIPRSKDGYYLTSLIRISDDGYYIDKLSELLYVREQKGKKEKYCNSGYQM
                     "
     gene            complement(15572..17362)
                     /locus_tag="PC471_001841"
     CDS             complement(15572..17362)
                     /locus_tag="PC471_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2788972.1"
                     /translation="MKKSKGFKKTIGRLLPFVKKYKFSFIIAIICIISAATMNALAPK
                     TEGLIITQLTKDVISIAKGVPGASVNFDYVTKILVILACIYFANAIFTYTSSFLLTNA
                     IQNTMRDLRNEVENKIRRLPISYFDSNSFGDVLSRISNDVDTISNALQQSFMQIVNSI
                     LVIILALSMMFTINIYMALIALFIIPISYFVSKFVVKKSQSRFSLQQNALGKLNGKVQ
                     EMYTGFNEIKLYGKEEDSIKEFKKVNQELCENGFKAQFISSMMNPMVSLVTYFGIAAV
                     AVVGSIYAVSGGITVGNLQAFVRYIWQINQPLSQMTQLSTVIQSSFAAIERVFEILDE
                     EEEIPDVENPVKIENVKGNVTFEHVNFGYGENSTLIEDLNAEVKSGQMVAIVGPTGAG
                     KTTLINLLMRFYDVKKGAIKIDGVDIRDMKRKDLRSMFGMVLQDTWLFNGTIFENIEY
                     GRFGATKEEIIQAAKVANVHHFITTLPDGYNMFLNEEASNISLGEKQLLTIARAFISD
                     PSILILDEATSSVDTRLELMLQKAMRNLMNGRTSFVIAHRLSTIRNADLILVMNNGSI
                     IEQGNHDELMEKGGFYEKLYNSQFADKESE"
     gene            complement(17362..19089)
                     /locus_tag="PC471_001842"
     CDS             complement(17362..19089)
                     /locus_tag="PC471_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003496244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2788973.1"
                     /translation="MKLILSYLKNYKLLIVLNILAIFSFALVELGIPTIIAKIIDNGI
                     ANQNIAYIKQMGIVIVIISIIGVVGSILLGYCSAKVSTSVTRDIRNDIFEKSQEFSHT
                     EYNKFGISSMITRTTNDAFQIQQFVNILLRTALLTPVMFIISIIMTIRTSVELSIVLA
                     ISVPFIIIGVAIIAKVSQAISSKQQKGLDKLNLISRENLTGIRVIRAFGNDDYETERF
                     EKTNTYYANVSKKLFKLMSITQPAFFLLLNIAVLAVFWISSEKINIGELQVGQLVAFL
                     EYLFHAMFSIMLFSMVFIMYPRAEVSANRIKELLNEEPLIKNPENGIKDTENKGIIEF
                     DNVTFTYPDGEASVLKDISFTAKTGETVAFIGSTGSGKSTLINLIPRFYDVTEGSIKI
                     NGVDIREYDLKALRKKIGFIPQKSLLFTGSIANNIRFGKHKAGESELEYSAKVAQAYE
                     FISKKPRKFDELISEGGANVSGGQKQRLSIARAIIRRPEIYIFDDSFSALDFKTDAIL
                     RAKLKKETKDAIVLIVAQRISSIIDADKIIVLNEGQVVGMGTHKELLKNCEIYYEIAT
                     SQLKKEELE"
     gene            complement(19334..19600)
                     /locus_tag="PC471_001843"
     CDS             complement(19334..19600)
                     /locus_tag="PC471_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HDF2788974.1"
                     /translation="MNLEPKKLIEQIGKEKDKKDIWIVGGGDIVKLFMKDNLIDDYYI
                     YILTIILGRGISLFKSGIDKTNLNFKKASNIGELVKLEYSKKQK"
     gene            19898..21010
                     /locus_tag="PC471_001844"
     CDS             19898..21010
                     /locus_tag="PC471_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HDF2788975.1"
                     /translation="MKISEVATPNFLLDLDQLEKNIDKIQQICTNNNKQLWPMLKTHK
                     STYIARLQKNAGASGFLVGTLDEGEALIKAGFEDIMLAYPYMGEVNINRIVTMASKIN
                     LICATDNIECAKAYSEAFSKSKIECKLLIIVDCGLHRFGVEPEKVVELATEISKLENI
                     KIVGISSHPGQVYGCSSPEGVPDVCVQEDSSMEKAYKALTDNGFNIEIVASGSTPTVE
                     SEAASKTITAVRPGNYVYYDAIQIGLGCATEDMCALTVVCTVISKNSSGYYLIDCGSK
                     CLGLDKGAHGNSSIVGYGRVCGHPELTIDSLSEEVGKIKVNGETDIKIGDRIRIIPNH
                     SCSTANLTSNLLGFRGDTIEKVIEVDIRENSKQIIL"
     gene            complement(21374..21883)
                     /locus_tag="PC471_001845"
     CDS             complement(21374..21883)
                     /locus_tag="PC471_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiG family protein"
                     /protein_id="HDF2788976.1"
                     /translation="MSQQNTGNVIVNSKPKKTAVEIFMGGAKKGFYIGVEQILPAMIM
                     GYVIVQFLQLTGLVDILGNIFGPVMGIFGLPGESVVVLIAAFFAKAAGAATAANLFAQ
                     GLITAPQATILIMPCMLMGTLVGHYARIVLVANASTKYRMLLLAVPIFDAIVGMLIVR
                     VLLTVMGLM"
     gene            complement(21885..22532)
                     /locus_tag="PC471_001846"
     CDS             complement(21885..22532)
                     /locus_tag="PC471_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2788977.1"
                     /translation="MDVTAGDNKVTWKGYLSLFVLIVLFSGVFKDSQGFLKALDFANL
                     TGSFGTIADGVDFMGKGGTGAKDGFLFALTLIPTVCFAVGLIDVVESMGGMKAASKIF
                     NPLLRPLLGIPGIAGIAFVSSFTSSDVASIMTKELFESGEITDDERTIFVAYQYAGSA
                     VILNTINTQAPLLPIALLALGPIILIEIFCKILGANLVRLIIGVRNKKNKAKEAV"
     gene            complement(22546..23718)
                     /locus_tag="PC471_001847"
     CDS             complement(22546..23718)
                     /locus_tag="PC471_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HDF2788978.1"
                     /translation="MNTYLNEISKFVDENREEIVSLWKEIVNMESYTHCKESVNKLAK
                     RLKFEFEKEGLDCELVDVGDNGSTLIGTLGSNIDKKPIIFSGHMDTVFETGTFGENPF
                     KIIEGKAYGPGVLDMKGGIIISLYAIKALNKIGYKERPIKIVFSGDEEIGHKDSTGAD
                     VILREAKGGLCAFNMETGLVDNSLCVGRKGRIGCNIHVKGVETHAGNDFEGGRNAIEE
                     MANKILRIQKLTNLDVGTTVSVSVIKGGRVENSIPEDCSIKVDLRFEKVEEMENVKEQ
                     VREICKETYIEGTSTEVNFISEMMPFETTEDVMRFHTFVNEVSKENGFGELNAKRLGG
                     SSDASYLTIANVPTICSFGVRGEWNHTSREYAVVDSMFERVKLISTVILNLDKFEV"
     gene            23986..25362
                     /locus_tag="PC471_001848"
     CDS             23986..25362
                     /locus_tag="PC471_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2788979.1"
                     /translation="MKYKIGFLTTSYFMSDKFKQALKELNDTCEITFIELKGENPFNN
                     LPNIYYEQVDNFDGFVCSGIVPYSELIINIKDIKVPLNFLKLDEKDFYKYLFKLLNSQ
                     KDIDFTKSFMDFLREDNNYYDIYSLIDSNKCPYTIKDFNIPQPVYDFKKLNDKLLEIH
                     LDLLRDNKINLSFTRNYIISCELNKLGYDSIYMVPSTESILNTFKSLVNEITLQNLDK
                     NKSATCIVTVNSSEYINEDLIFKNDEIQNQIYNTILNSLSRHGFYGVQVKKNDMKIEI
                     HTTKEMLNNMTNNYTDFFISEDLKEIKYSLNIGWGIGNTNIHAEQNARQANGKSASLN
                     GNCTFIVNDTNDTIGPLYSNKNSNNIEDSSIANKVASFIPLSNTNVSKIMCMINDRNS
                     NNVSAEILADYMNITLRSANRILSILYKAGIATIVNTKLDNQRGRPKKIYKVDFLSFL
                     NKMQKLNS"
BASE COUNT         8814 a         4441 c         3059 g         9781 t
ORIGIN      
        1 tataataatt gaaaataaaa gaggatacct cataatgata aaagaatcac tttgagatac
       61 cctcttacaa acaactaaat caataatttt ctatctttca cactcttgat ttgcaactgt
      121 acaagaagtt ttacaagctg attgacaaga agtttgacac tctccacatc caccttttgc
      181 agcactttgc tttaaagtag cttgagataa agtttttaca tgtttatttt ccattttcaa
      241 cgcctccatt tctaattgtt tcctattgat tatatcataa aactacttta atataaactc
      301 tatttaaagt tgacacccaa tacgcctcca atgcctcctg ctgctgatat taacaataac
      361 ttataaatca tccctacttc aaatacaaac ttttcttgtg ctaaaaacac agtcaaaaaa
      421 atacatacta tgtacaaaag tcctactaaa agcccataca taagtccttt ttctttcatg
      481 tgttttgaag tgtaaaaccc tcctatagct gcagaaatag tagttataaa tgatgagacc
      541 atagttatat tatctccacc aatatctgta aatgtcaaaa ataagttata cactaataaa
      601 acagccaaag ttattatata tgcatatcct agacctttaa aaatataatt agattttccc
      661 ataaaaaaac ctcctctcaa attaatatat attcgagaaa aggcttttaa attactaatt
      721 tagtcttctt ctatttcttc ttttttagat ttaacagttc ctatagccca tttttcaaat
      781 ggaactttag ttctatttgg tcctaattct aatattacaa catcatcttc aactttagct
      841 acattagcta ctattccacc tattgtaatg accttgtctc ctacacttag gctacttctc
      901 atttctttta attgtttgtc ttttttcttt tgaggtctaa tcattaggaa ataaaagatt
      961 gcgattacta ctacccatag acctatactc ataattattt gttgctgtgg cattaatttt
     1021 tcactccttt gtaaattata ctcttttgta aatcataata aatgtttata taacaattta
     1081 ctttctctat ctttgagtac tattgaatat tatatattaa aatgattata attaatagtg
     1141 caaaacctat atttcatatc catattttgc aaaaaattca tttctaaaat caagtaatct
     1201 atcttccatt atcgcttgtc ttatttgttt cattaaattt aataaaaagt gaagattatg
     1261 agtagttatt aatcttgcac ccagtatttc atttgctttt ataaggtgtc ttatataagc
     1321 tcttgaatag tttttacaag cataacaatc acattcaggg tcaagtggag taaagtcttc
     1381 agcataagtt gcatttctaa ctactacttt accttggcta gtcattgcag ttccatttct
     1441 agcgatacga gtaggtagga cacaatcaaa catgtctact cctcttataa caccttctac
     1501 taagtcatct ggactaccaa ctcccattag gtatcttggt ttatcctttg gcataagtgg
     1561 agttgtatgc tctaacacgt catacataag tggttttggt tctccaacac taagtccacc
     1621 aatagcatat cctggtaaat caatattagt tatttctttc gctgattgtt cccttaagtc
     1681 tttatacatc ccaccttgta ttataccaaa taaagcttgc ttgtcggtat tattatgggc
     1741 atctttacat ctttttaacc atcttgttgt tctttctaaa gaatttttta catattctct
     1801 atctgatggg tatggtgcac actcatcaaa tgccatcatt atgtctgaac ccaatgcatt
     1861 ttgtatttcc atagcttttt ctggagttag aaaatgtttt gaaccatcta agtgtgacct
     1921 aaattctaca ccttcttctt ttatctttct taaaggccct agactaaata cctggaaccc
     1981 tccactatct gtaagtattg gtttatccca attcataaac ttatgaagac caccagctct
     2041 ttttataagt tcatgacctg gtctcatata taaatggtat gtgttactaa gtattatttg
     2101 tgaaccaatc tcttttaatt cttctggtgt catagatttt acagttgctt gagttccaac
     2161 tggcatgaat attggtgttt caataatccc atgtggagtg tgtaatcttc ctaaccttgc
     2221 accactctgc ttacaagtct ttatcaattc gtatcttact gcgtacataa agttctcctt
     2281 aatcatattt atttctcatt atttcttaag atatatttgt aataagctat tcatataatt
     2341 tattgtctaa atagatttat cttcgtcttc tttgttctgt tgttccttaa ttgcttcttc
     2401 atcatgaata ctcattatat taagagcact cttgtctttt gttactaaaa acttttttat
     2461 agcaaatagt cctgctatag ctataactcc tattaaaaca tctatcatac cttcattttt
     2521 aggcagtagc aacatttttc tagcaattgc gtaaagtaat gcctcaataa atgctcctgg
     2581 tatatgaaga gatagcatta ctacaagttc aacacctatt actaaaagta atatttgtgc
     2641 taaaaaatca ttaagctgac tgtattgtac tggattttga aaatcaacta tatatgctcc
     2701 ccacataagt cttagaacat ctatagttcc taaaaatact gctattagaa ctactactgc
     2761 taaaacacgt tcaaatataa aagcaatctt taatataggg ttattctttt tttgtttttg
     2821 taaactatgc atctattacc tccctacttt ataatcatag catctccaaa actgaagaat
     2881 ctatatcttt cttttactgc ctcattatat gcatttaaaa cattatcctt tccacaaata
     2941 gcacttacta acattattag tgtagactct ggtaagtgaa aattagtaat aagtttatct
     3001 aaaactttaa atttataacc tggatatata aatatttctg tccatccact agtttctttc
     3061 atctttccat cctcattaca agctgattct atagttctac aactagtagt accaacacat
     3121 attacatttt ttccattttc ctttgctcta tttattttgt cagctgcttc ttggctaacc
     3181 atataatatt ctgaatgcat cttgtgattt aaaacatcct ctacttttac tggtctaaaa
     3241 gttcctaagc caacatgtag agttacaaag gctatatcta cacctttttc ttttatttta
     3301 tttaacaatt cttctgtaaa atgtaatcct gctgtaggtg ctgctgctga accattatgc
     3361 tttgaataaa cagtctggta tctttctttt tcatctagtc tagcagtgat atatggaggt
     3421 agtggcatat ttccaagttc atccaatatt tcttcaaaaa ttccatcata atggaatttt
     3481 ataattcttg aaccttcatc tgataaatct acaacttctc caattagctt tccatttcca
     3541 aaagaaaatt tagtccctac ttttgctctt tttcctggtt ttactaaagc ctgccaagta
     3601 tcttcctctg ttctttttaa taataaaaac tctattttac ctccagtttc aagcttctct
     3661 cctattaatc tagctggtat aactctagta ttatttaata ctaagcaatc tcctggattt
     3721 aaatattcta ttatatctct aaatacttta tgttcaatat tacctgtctc tttatctaat
     3781 accatcaatc ttgagctagc tctatcttct ataggaactt gagctataag ttcctgtggt
     3841 aagtcaaatt taaaatcact tgttttcaat gttaaacttc ctctcttaaa atttctccag
     3901 ctacaataat atcaaaaaaa caacttattt tccacaatat taccataatc tatcaagaaa
     3961 ttgctatttt taacagacac catatccaca atatatagat atttttccac gatatacatg
     4021 ccttatccac aatatataga tacaatacac acaattttta tcgtgtttat ccacagtatc
     4081 tacagatata attaccattt tgtggatatg tttgaatttt tagccatttt agaaatattt
     4141 taatttaatg actcttttat tttataaata ctcgtataat ctatttttat ttttcattgg
     4201 gatatggaat ttttaagtgt tcatatgcta atggcatagc tactcttcct ctaggacctc
     4261 tatttataaa tcctaattga agcaaatatg gctcatatac atcttctatt gtatttctat
     4321 cttctccgat agaagctgcc aatgtatcta aacccactgg tcctcctcta aatttttcta
     4381 ttatagtcat aagaagcttc tcatctacaa aatcgagacc caaactatcg actcctaaaa
     4441 gttccagagc atcttttgcc actttatcag ttatttttcc atcagctctg acttgtgcaa
     4501 aatctctaac tctttttagt aatctatttg ctatccttgg agtccctctt gaacgttttg
     4561 caagttctaa tgctccatta gactgtatct ctgcatctaa gatgcttgaa gacctaagta
     4621 ctatttttga aagttcatca actgtataat agtccaattt acaaataaca ccaaatctat
     4681 ctctaagtgg atttgtaagc ataccagctc ttgtagtagc tccaataagt gtaaatttag
     4741 gaaggtcaag tcttatgctt ctagcagatg gccctttccc tattattata tctaggcaaa
     4801 aatcctccat tgctggatat aaaacttctt caacacttct attgattcta tgaatctcat
     4861 ctataaataa aacatcattt tcatttaaat tcgtaagtat ggctgccaaa tctccagctc
     4921 tttctattgc aggtcctgac gtaattctta aatttacccc catctcattg gctattatac
     4981 ttgctagtgt agtttttcca agacctggtg gtccatataa aagcacatgg tctaactgct
     5041 catttctcga tttagctgct tctataaata tactaagttg ttctttggat ttttcttgac
     5101 ccaaataatc ttctaaagtt tttggtctta aactgttttc tatatctata tcttccatct
     5161 tcattgtaga agttattatc ctattttcat cttcaaaacc ttgcatttat acccctcctc
     5221 tcgtactttt gaaaatctac tttatatatt ttcaaattta cataaactcc ttaaatattg
     5281 ttttaaaatc caaatattat aaaatccaaa tactatttca aggatttact cattatatat
     5341 gtaagtgctt tctttataat agcttctgta ttcataccat cttttttaca tttttgaact
     5401 gcctcttttg attccgacaa agtatagcct aaagctacaa gtgcatctac agactcatct
     5461 tgtgatatca aggtaggttt ttgtgataat aacgttggtt caaactctat gttatttttg
     5521 tctaccttat ccttaagctc taagacgatt ctttctgctg tctttttacc aacaccagga
     5581 gctttggata attttccaat gtcttcactt aaaatatatg ctcctaactg tgctggtgat
     5641 gcaaatgata gtattgaaag cgccacctta ggtcctattt tagatacaga tgttagtaat
     5701 tcaaacatct taagttcttc cctacttaca aaaccacaaa gactcatatc atcttctctt
     5761 acaattaatt tagtatatat ttttgtggct tctcccactt gtaaattcat tattgtattg
     5821 gatgatacat ttattttata tcctatccca ttattttcaa caactatact gtctatataa
     5881 atttcttcta ctgtaccttt tatataacta tacatactta ccacctatat tctttagagt
     5941 tttttcaagt ttgtttgaat gagcatggca aatagccaca gctaaagcat ctgcaacatc
     6001 atctggtttt ggaacttttt ttagacttaa aaatgaagtt accatctgtt gaacttgtgc
     6061 cttatctgct ctcccatatc caactacacc ttgttttact tgaagtggag tgtattcata
     6121 tacaggcttc ccattatgag cacatgcaag catagtaaca cctctagctt gtgcaactgt
     6181 tatagctgtt tttacattct tgttaaagaa taattcttcc attccaactt catctatatt
     6241 gtaattcttt actattgtat caattccttt atacacaagt tccaatcttc tcgatatatt
     6301 catatgggca ggtgttgtaa ctgctccata atcgattgct ttaaacttgc tatttttgta
     6361 ttcaattata ccatatccaa ctatggctat acctgggtca atccccaata ttatcatttt
     6421 ttaaaattcc ccctaaaaat aaaagaaata ccaaaatgta tttctcataa aaacttatgt
     6481 tctaaatcat tatatcataa ataatattaa caaatcataa aatgctctat gtaaaatata
     6541 aaatcaaata aaaaagttat atcaaagcaa gattttacct caatataact tttatattta
     6601 ttacaaataa attattgtat ttttatagga attaaatgtg actacaattt taatactaat
     6661 ctatttaata ttaaattatt tattattaat ttacttagta tttaacttaa aataatatta
     6721 atttactatt aacttgcaaa gtcttctaat cttgaatagg cctcttcttc agtatccata
     6781 actacacctt ttttgatttc atcttgaact gttttaggaa gtgaatctac tcttattttt
     6841 gttttttcta ctaatgtttt acttccgcta tcatcatatt tatatagtgc tatatttcca
     6901 ttatcatttt caatagtata tttacctttt atagtaacat ccattgcttg tacatcttta
     6961 ttatcactat atttagattc taccaatatg atttcatttt tatccatact ctttattttt
     7021 ttagttggat attctttttt aaaatatgat actatttctg acttcgtctt atctaataat
     7081 gactctggaa ctattcctgc cattgtaagt gttctcttat agttagtttc attttcgcct
     7141 tcactttgaa gccaaacttc acaattttta tttaatccaa aagcttcttt tgacttttca
     7201 ttcatattcg cactagtact tacaacttca ccagtttgaa ctccattgtg ttttaatctt
     7261 gtccctatat aaactccgct acttaataca aatactccaa ccagtattaa ggcagatatt
     7321 ttccaaggaa ataattcttt tttcttcttt tcaaacattg attacgcctc ctttttctat
     7381 tattttctcc aagctgtaag tttttataca atttaggtaa attttagaag aaaatatatt
     7441 tatatttcaa attactctaa gaatagatat tttctaaaag gtagttttct cttaatggtt
     7501 atattcttgt aattatatgg acataattta taataaataa tcgtttagga ggtaataact
     7561 ttggaaagaa taaactgtaa tgtaattaac tgttcacata acaacagcca tatatgttat
     7621 gcagataaaa tttttataaa tggtgaaaaa gctcacaatg acagacatac ttgttgtagt
     7681 tcatttttag atgaatctat ttatagtaat ttatctagca acgttaataa taaaaattgt
     7741 gaggaattag tttgtactgt aaaaaattgt aaatacaaca aagatggtag cttatgtaca
     7801 cttgataata tattggttgc tcctgaaact gatagagtaa acctttatat tgaaacatgt
     7861 tgctctagtt tcgaatgtaa atagtcatag tataaatata agggtgtcaa atgtaattaa
     7921 actaacatag tgtagagtat ctacaactat tagtttgatt atacttgaca cccttattta
     7981 ttttaaataa aatttcatta ttataaccat gtttataatg ttctaactgt aattaaattt
     8041 gataaatttc tataaattgg tataatcaat atgtaaattg aatttagtaa attaatcata
     8101 aaaataaaat atcgagtata tagtgttata ctaactagaa atgaggtgct tatgagtaca
     8161 ttttatattc aatttcttta ttttgtaatt tatagcttta taggatggtg ttgtgaaact
     8221 acatattgtt cagttttaca aaaacaattt gtaaatagag gttttttaaa tggtccattt
     8281 tgccctatat atggatttgg agcattagca atagttgcta ccttgactcc ttttgtacac
     8341 aatattcctt tgttattttt gtttagtata attataacta gcataatgga gtactgtacg
     8401 agctttattt tagaaaaaat atttaatatg acttggtggg attattctaa acataaattt
     8461 aatattcatg gaagagtttg tctggaaaac tcacttatgt ttggtgtatt atcattgatt
     8521 gtaatgctta tagtacatcc tattgtggtt gactttatta actctatctc taaaaatgtg
     8581 ctatttatat ttgctatatc tatagaaatt tattttgttt tagatttagt tatcacagtc
     8641 catacaattt tacaattaaa tggtaagtta aaacaaatca atcttataat taaggaatta
     8701 aaagataaga aagaatacta caaacttatt acacaagaaa ctatagaaaa caaacttgaa
     8761 tctttagttg aaaatagact ggatactttg gaagataggt ttgaaatact taaagaaaaa
     8821 gaaaattatc gctatgctaa aaatcgtatt gatgagctaa aaaataaact taatactttg
     8881 ctgtctaacc acaaattact tcatagacgt ataatcaaag cattccccaa cataagctct
     8941 aataagcata cagatatttt aaataaaatc aaagaaaata ttaaatcaat taaaaaagat
     9001 aaataataaa ataaacacat aagttaaata ataaaaatac taatacataa gattaaggtt
     9061 tagaatattt tttgtaaaat actctaaacc ctaattaata tactattatt tttaatctta
     9121 atttataatt ttagtttatt ttaattttaa ctcagtaaat ttaagcgttt aaacttcaat
     9181 acactaatat actaccccat caatatctca actgattctc taagtgtgct aatctctccc
     9241 acatttccag ggaaaattat atatggcata ttaggaaatt tgctttcttt gcctgtcatc
     9301 catactggta taccaggttt tatctgtccc attactgtag ctttttttac tcttagtgct
     9361 ttagtaccaa catcactaga agtaattcca cccttagcta taataaaatt tggtcttaca
     9421 ttaagcatac caattatact ggtaactgca tctgatattt ctacagaaat cataagctgt
     9481 ttatctttat cattcgtatc caagtcaaaa cgctctcgtc tagtataaac agcaacagtt
     9541 ttaccactgc ttattttttc ctctacaact tttataactc tttttacctc attttttaga
     9601 ccattttctt gaagaactaa atgttgatta aattctataa attctatcgg atatttacaa
     9661 tttttaagtt cttctaactg ttgggtagtc ttattcacat gtgaaccaac aagtataatt
     9721 cctccattgg tattaccttt tgatactaat tcatccttag taagtagttc cttgtcactc
     9781 actccgccaa gtacttttgt tatggcagca gcgcttctaa acataaattc cttaccacca
     9841 tttactgctc gtataaacgc aattgtaaat acctttacat ctacataatc ggttgcattt
     9901 acaataattt tattgaatcc ttcagctttt ttaagttttt ctgttatagc ttcaatattt
     9961 agctctctaa gttcttttga cgaaatatat atcatatcac tagatttgta ctttccattt
    10021 gtcttttctt cacaccactc acctatatca gatgatttat atccaaaaga tttatctttt
    10081 gcaaattcag tcattcctgc tggtacaagt atttctcctt ctttaacata atgtacatta
    10141 tctatagtaa acctaccacc ttctttaaaa aatggcatta ttatttcacc ctgaaataac
    10201 ttccccgata atctctctac ttcgcttttt aataaattag tttcaagagg ataatgtcct
    10261 ctcatagtag aatcactacg acttatgatt ataaaatcct tattaacttt ctttgataca
    10321 tccacaatat tctttgaaat ttctttatgt gcttttgtag tttgagcaac agtaaatccc
    10381 cttgaattgg taagtataaa aaacatacta tttttctcat taaagccttg ttccatacta
    10441 tccttatccc aatcagtata aacagaaata tcatgaactg tttgtacacc tgttgggtca
    10501 tcatctaata caacaatttt tctatctaaa tgcttaagct catctgcaag aagtttattt
    10561 aatctatcct catctacttg tttaaaagta ttaagtatat ctatgctaat tggttttact
    10621 tctctcattt aaaacgcccc tttatgtaat ttgtatagtc taaatataat ttatatgata
    10681 taaacaatag tttgttattt atagacctgg aagtggtata attccattta taacactaag
    10741 caataaaacc attataaatg ctacaactcc agcaatggtt cctccaattg tccaagagtt
    10801 taaagtatct ttaactgata atccactaaa tcttgaaagt acccagaatc cagaatcatt
    10861 tggaagggag aaaccaactc ctccagcaca tatagcaagc cctactaaaa ctggtgacat
    10921 tccagttgcc aaaattgtag gtcctaaaat tgatgatgtt gttactaagg ctacagtagc
    10981 agaaccttga gaaattctaa gcaaggcact taataagaat ccaagtacta caactgatat
    11041 actaagacca gacatagtat caactaagaa gttacctata ccacttgcat ttataacact
    11101 gccaaacgca ccacctgacc ctgttatcaa taatattagc cctgctgaat cagcagcttc
    11161 aataactaca tctcccatag gtttatttat gtatttcttt aaaaatccta ttgccaccaa
    11221 tattccaaga agtattgcta tgtttttatc tcctataaat gcaagtactc cactcattgt
    11281 agaacctttt tctaagaaca tactaaatac atttgatata agtataagaa gtattgggaa
    11341 caataaagaa aacatagaaa ttccaaaacc tggtacttta gaagaatcat catcttcaat
    11401 ttttatgcta tcttttccct gctctaaatt tttttctcta tcattctcat caatagcata
    11461 agctggatat ttttcaaaac gttttccata aatccatcca cctataagag ctgctggaaa
    11521 agcaacaatt atagaataaa gtataaaact acctacattt gcatttacat ttgctgcaac
    11581 tgccaaaggt cctggtgtag gtattacaag tgcatgacct actatagtcc caactcctaa
    11641 ggcacataca aaaaccatta gagatttctt agttactctc gaaacatact taattatagg
    11701 catcattata acaaaagctg catccataaa aactggaatt gaaattaaaa aacctgctgt
    11761 agttactgct aaagcactgt ttttttctcc agtcttagct aacaacccct ttgcaataga
    11821 ttctgttgct cttgattctg aaagaatatt tccaaatatt attccaagtc caattacaat
    11881 acctatacta cctaatgtat ttccaaatcc agtcgatatt gtggttgata tttcatttat
    11941 aggcattcta accataaagc ctgtaattat agatgttatc agtaatgcca aaaacggatt
    12001 tactttaaat ttaataatcg atacaagtaa aattcctata gatataacaa atgctatcaa
    12061 aagtccatat cctgtaatca taatctttac ccctttatca ttctacttta ttatttatca
    12121 attttatttt tcttttacta tgataactta acctcgctgt atttatataa tctaatccta
    12181 ataatatgta tttaattata ttgatatatt taaatattaa ttatcgtgtt tttttatttc
    12241 aacacctgcc aacttttcaa aatactgtgc tattccacta tggtcatctt ccaaatgtcc
    12301 atgtactttt aatgtctgca taatttctaa taattgactt gtcataggta gtggtacatc
    12361 taaattgtgt gctgtttgca ttacattctt aatatctttc aaattaatag atatttttcc
    12421 acctggttta aaatcacgat tcataatcat tggtatcttc acatccaata ttgtactacc
    12481 agctaggcct cctcttattg ccttatatac tttttctggg tctgcacctg cttttgctgc
    12541 aagtacaaat gcctcagaaa ctgctgctat cgtcaaatta actattactt ggtttgtaag
    12601 ttttgtaaca cttccactac cattatctcc aattaaaata gatgagcttc ccattatatt
    12661 aaaaaatggc tctactttct tataaatttc ttccttacca ccagccataa aagcaagtgt
    12721 accatcaatc gcttttggct cccctccact tactggtgag tctataaatt ctaagcccat
    12781 atcttttaat ttatttgcac acaatattga ttctgtagga gtaactgaac tcatatcgac
    12841 tactatactt ccttctttta aagtttttgc caatccatcc atcccaaaca atatatcctg
    12901 cacaatagtt ccatttggaa ggattgtaaa tacaatatca cattcttgac ccatttctaa
    12961 aacacttgcg tattttgctc cacatgatat aagttcatcc acagcagatt tattaatgtc
    13021 atacaccaaa agattacatc catcttttaa taaatttttt gccattggct ttcccattat
    13081 ccctaatcca ataaatccca atttcatttt ataaccctcc atatcttcac atttttgaaa
    13141 acgtttttta tgttttagag tatactacat accagtatac cggtcaatat aaaatataaa
    13201 agtatttatt ttaacaataa atagtaattt ttaaagtcat ttattatatt tagtcatcct
    13261 atataagttt taatacatac tattgttttc aaatataata tactactttt acagttttgt
    13321 attttaacta atttcaatta gttttaatta atctgccttt aatgttaatc atgcttcaca
    13381 cttcaataat tttttatatt ttttaatctt atctgcaatt taaattgcaa aagattgacc
    13441 ttattttcac tatgttgtat gcttaaaatt atataagttt ttgtattttt atattgttta
    13501 ggggggtatt attaagtatg gctacattta aaagattaca agaacaagca tatgactact
    13561 taaaagaatt gatactatct ggagaaatga tagaaaatga aatatattct gaaacaaaac
    13621 ttgctagtga aataggtatc tctagaacac ctataagaga tgctcttcaa agacttagtc
    13681 aagatggatt tatagatata atcccaagca aaggatttag aatccatcaa attacagcaa
    13741 atgaaattgt agagatattc caaatacgct ctgcaataga aggattttgt acttttttaa
    13801 ttacaagtca atacaaagaa gctagagcag tagaaactat aagtaaatta aaacatcttt
    13861 tagataaaca aaaagatatt ttattaggtg ataaaaattt aaactcattt gcagaatacg
    13921 atacattatt tcacacaact atagtttcat atgctcaaaa tactgaattt gacaaaatgt
    13981 ttaacaatta tatgtacaga ataaaaaaat tagctctcga ttcattgtca catgaaggaa
    14041 ggcttgaaac aacattaaaa gagcacacag atatttttaa taatattgca aatggatgta
    14101 ttgaagatat ttacagaact actctcattc atatggaaac tcctaaatac attaatttag
    14161 aagatttttg taacaataag ttattttagt attatacttt catttattaa aaatatatct
    14221 atctcaaatt aatttcctaa tttataaaaa atattctata attatattgt aactaatact
    14281 tattctaagg agtgattttt ttgaacgaag atgttgtaac taatgaaact attgacaaag
    14341 attatgctat tgaagaagtt cgtatggcat gcaaacattt tggtgattta tatttttatt
    14401 tttccaaagt gttatttgaa gagtttggtg aagataaaac tagcgaaata ttaagaaaag
    14461 ttttatttga aaggtctgaa gaaagagcta ttgctatgag agagagagct catgaaaatg
    14521 gagatgaatt aatcgcagac aatattatta gcacaactga tgttccattt ctaggatggg
    14581 ttcctgaatt tgaagagtta cattgcccct atggagcttc ttggctttta agatttgagg
    14641 aaaatccttg gtttaaaaaa tttgcttctc tttactgtga tgttacagat actactgtag
    14701 ctgaagtatt tacaggagat acgtcccata aaataacaaa aaacattcta tggggagata
    14761 aaagttgtga aagaatatat ttccatgatg acaaggtatc tgaaggtaaa tatacttatg
    14821 gaaataaaaa ctgtaaataa atgatttaaa ttttctatat tcatatacta tattttgtac
    14881 tttttcatat aaatattata actagtcttt tattttactt ttgtttaacc taaagataat
    14941 ttacttttaa tctatatgaa ttatcatata aataatcaga aagttatctt tataaattat
    15001 agttttagat ttataattta taaaatattt atgttctttg taaaattttt tagaagcttc
    15061 taatgttatg ctataaaact atcttgagaa gggctgattg aatgtttaat aaaatatatg
    15121 aaaagaaaga atttatagtt tttcaagtta aaaaaggtta tgtagtttat aatactagaa
    15181 agagttttga agaaggtcat acacatttaa agcattttga agcagcaaag acagctattg
    15241 atttagcaat aaataaaaag attcctagaa gcaaagatgg atattatctg actagtttaa
    15301 ttcgcataag tgatgatggt tattacattg ataagttaag cgaattgtta tatgttagag
    15361 aacaaaaagg taaaaaagaa aagtactgta attctggtta tcagatgtaa caagactagt
    15421 tgtgaattat aagtaccaat cataagtacc tttagtctta aaatcaaaga gtatcagtaa
    15481 atctataaaa aaacaactta ctgatactct ttttaattat atgattaata tttgaatttg
    15541 atacaaaatt ttatacaaaa gtttatgtta tctattctga ttctttgtct gcaaattggc
    15601 tattataaag cttctcataa aatccaccct tttccattaa ttcatcatga ttcccctgct
    15661 ctataatact tccattattc ataactaaaa tcaagtcagc attgcgaatt gtagaaagtc
    15721 tatgtgctat aacaaaacta gttctaccat tcataagatt tctcattgct ttttgaagca
    15781 taagttccag cctagtatca acagaactcg tagcttcatc aagtatcaat attgaagggt
    15841 ctgatataaa agctctagct atggttaaaa gctgtttttc ccctagagat atattagatg
    15901 cttcttcatt taaaaacata ttgtatccat ctggaagagt ggttataaag tgatgaacat
    15961 ttgctacttt tgctgcttgt ataatttctt ctttagtagc cccaaatcta ccatattcta
    16021 tattttcaaa aatggttcca ttaaataacc atgtatcttg aagcaccata ccaaacattg
    16081 aacgcaaatc tttacgtttc atatctctta tatctactcc atcaatttta atagcacctt
    16141 tctttacatc ataaaacctc atcaataagt ttattaatgt tgttttacct gctccagtag
    16201 gtccaacaat agctaccatc tgaccacttt ttacttctgc atttaaatct tctattaaag
    16261 ttgaattttc tccatatcca aaattaacat gctcaaatgt aacatttcct tttacatttt
    16321 ctatcttaac tggattttca acatcaggta tttcctcttc ttcgtctaaa atttcaaata
    16381 cacgttctat agctgcaaaa gaagattgta ttactgtaga taactgagtc atttgtgaaa
    16441 gtggctgatt tatctgccat atataacgca caaatgcttg taaatttcct actgttattc
    16501 ctccacttac tgcatatata gaaccgacaa ctgccacagc tgcaattcca aagtaagtta
    16561 ctaatgaaac cataggattc atcatacttg atataaattg tgccttaaat ccattttcac
    16621 aaagttcttg atttactttt ttaaattctt ttatagaatc ttcttcttta ccatatagtt
    16681 ttatttcatt aaatccagta tacatttctt gtacttttcc atttaatttt ccaagagcat
    16741 tttgttgcaa tgaaaatcta ctttgagatt tttttactac aaatttagat acaaaataac
    16801 taattggaat tataaaaaga gctataagag ccatatatat attaattgta aacatcattg
    16861 aaagtgccaa aattatgact aagattgagt ttacaatttg cataaaactc tgctgcaatg
    16921 catttgatat tgtatctaca tcatttgata tacgacttaa tacatctcca aaactattac
    16981 tatcaaaata actaataggc aatcttctta ttttattttc gacttcattt cttaaatctc
    17041 tcattgtgtt ttgaatagca ttcgtaagta gaaaacttga agtatacgta aaaattgcat
    17101 tggcaaaata tatacatgct aaaatcacca atatttttgt tacataatca aaatttacgg
    17161 aagcccctgg tactcctttt gcgatactaa ttacatcttt agtcaattgt gttattatca
    17221 atccttcagt tttaggtgca agtgcattca tagtagctgc tgaaattata catataatag
    17281 ctataataaa ggaaaattta tatttcttta caaatggaag aagtctacca attgttttct
    17341 taaaaccttt agactttttc attattctaa ttcctccttt tttaactgag atgttgcaat
    17401 ttcatagtat atttcacagt tttttaataa ttctttatga gttcccattc ctacaacttg
    17461 tccttcattg agtactataa tcttatcagc atcaataata gaactaattc tctgggcaac
    17521 aatcaataca attgcatctt ttgtttcctt cttcaattta gcacgaagta ttgcatcagt
    17581 tttaaagtca agtgcagaaa aactatcatc aaaaatgtat atttcaggtc ttcttataat
    17641 agcccttgca atagaaagtc tttgtttttg acctcctgat acatttgctc caccttcact
    17701 aatcaattca tcaaactttc taggtttttt tgatataaac tcatatgctt gtgcaacttt
    17761 tgcggaatat tctaattctg actctcctgc cttatgtttc ccaaatctaa tattatttgc
    17821 tatgctacca gtaaaaagca gtgatttttg aggtatgaat cctatctttt ttcttaaagc
    17881 ttttaaatca tattctctta tatctactcc atttatctta atgctacctt ctgttacatc
    17941 ataaaatcta ggtatcaagt taattagagt acttttccca ctaccagtac ttcctataaa
    18001 agcgactgtt tcacctgttt ttgcagtaaa tgaaatatcc ttaagtacag aagcttctcc
    18061 atctggatag gtaaaagtta cattatcaaa ttctatgata cccttatttt cagtatcctt
    18121 aattccattt tcaggatttt ttatcaatgg ttcttcattt aatagttctt ttattctatt
    18181 tgcagatacc tctgctcttg gatacataat aaataccatt gaaaaaagca taattgaaaa
    18241 catggcatgg aaaagatatt ccaaaaatgc aaccaactgt ccaacttgaa gctctccaat
    18301 atttatcttc tcacttgaaa tccaaaatac agccaaaaca gctatattta atagtaaaaa
    18361 gaatgctggt tgagtaattg acataagctt aaatagtttc ttagaaacat tagcataata
    18421 tgtatttgtt ttttcaaatc gctctgtttc ataatcatca tttccaaaag ctctaattac
    18481 tcttattcca gttaaatttt ctctcgatat aaggtttaat ttatctagtc ctttttgttg
    18541 ttttgaagat attgcctgag atacttttgc tattattgct actccaataa ttataaaagg
    18601 tactgatata gccaatacta tagaaagttc cacacttgtt ctaattgtca ttattatact
    18661 tataatgaac ataactggag ttaataaggc agtccttagt agtatattaa caaattgttg
    18721 aatttgaaat gcatcattcg ttgttcttgt aatcattgaa gaaattccaa atttattgta
    18781 ttcagtatgt gaaaattctt gtgatttctc aaatatgtca tttcttatat ctctggttac
    18841 acttgttgat accttagcag aacaataacc caacaatata ctacctacaa cccctataat
    18901 agaaataata acaatcacta ttcccatttg ttttatgtaa gctatattct gatttgcaat
    18961 accattgtca attattttag ctattatagt tggaatcccc aactcgacta atgcaaaact
    19021 aaatatagcc agtatgttta atactattaa taacttataa ttctttaaat aacttaaaat
    19081 taatttcata tttttctcct ttcttaattt ttgagatagt gtaatcatgt aataatacta
    19141 aatttataaa tgcttaattt tataaaaaaa ggagaaaaga gttaacgcac tctcttctcc
    19201 agcatattct ttttaataca ccctccgaca agtcgcttat atgttaacat atttgacaag
    19261 taatgtcaat tcatatagcc aaaagtcttt taaatgcttc gtttcataac attgtaacaa
    19321 attggcatgt aatttatttt tgttttttac tgtattccaa ttttacaagt tctccaatat
    19381 tactagcttt cttaaaattc aaattagtct tatctatacc agacttaaat agtgaaatcc
    19441 ctctccccaa tattattgtc aatatatata tataataatc atcaattaag ttatctttca
    19501 taaataattt tactatatcg cctccaccaa ctatccaaat atctttttta tccttctctt
    19561 ttccaatttg ctcaattaat tttttaggtt ctaaattaat ttatagtgtg attattctat
    19621 gtaaacatca cttttttact cattcaataa taccctatta ataaatcctc aaactaattt
    19681 aaataaactt tgtataaaat taaaaatata ttatataaat aatttaaaac tacactaact
    19741 tattgaaaat tctaattatt acaaataaat attgctctct gttaacgcta gttctataat
    19801 ataattataa gctattaata ttataaagcc gatattacta atataataat tataatactt
    19861 atagttaatt tacttttgtt gaaaggtggt aaataacatg aaaatttcag aagtcgctac
    19921 tccaaatttt ttgctagatt tagaccagtt agaaaagaat atagataaaa ttcaacaaat
    19981 ttgtacaaat aataataaac aactttggcc tatgcttaag acacataaaa gtacttacat
    20041 agcgagactt caaaaaaatg caggtgctag tggttttcta gttggaacac ttgatgaagg
    20101 tgaagctctg ataaaagcag gttttgaaga tattatgttg gcttatcctt acatgggaga
    20161 agtaaatata aatagaattg tgactatggc atcaaaaata aatttaatct gtgctacaga
    20221 taatatagaa tgtgctaagg cttatagtga agctttttct aaatcaaaaa tagagtgcaa
    20281 gctacttatt attgtagatt gtggcttaca ccgttttggg gttgaaccag aaaaagtggt
    20341 tgaacttgct actgaaattt ctaagctaga aaacatcaag attgttggta taagttcgca
    20401 tccaggtcaa gtgtatggtt gtagttctcc agaaggtgtt ccagatgttt gtgttcaaga
    20461 agattcttct atggagaaag catacaaagc tctaactgat aatggattta atatagaaat
    20521 tgtcgcttct ggtagtactc caacagttga atctgaagct gcttctaaaa ctataactgc
    20581 tgtaagacct ggaaactatg tatactatga tgctatacag ataggtttag gttgtgctac
    20641 tgaagatatg tgtgctctta ctgttgtgtg cactgtaatc tcaaaaaata gcagtggata
    20701 ctatttaatt gattgtggaa gtaagtgcct tggacttgat aaaggtgcac atggaaatag
    20761 tagtattgtt ggttatggta gggtatgtgg acatccagaa cttactattg attcactttc
    20821 agaagaagtt ggaaaaatta aagtaaatgg tgaaactgat ataaaaattg gtgatagaat
    20881 aagaattata cctaatcatt cttgttccac tgcaaacttg acaagtaatt tattaggttt
    20941 tagaggagat acaatagaaa aagtcataga agtagatatt cgtgaaaatt caaaacaaat
    21001 catcctttaa aaacacattt attctgatat taacatgaaa gtatattttt cttaaaatta
    21061 ttgctaataa ctaatgttaa tgttggataa ataaaaaaca taaattctag aaatataaat
    21121 ttaaaagaag aggctgtctc aaaatagaga taagcctctc tttcattgtt gaaattatga
    21181 aaatatactt gatttcatac aaaaagagta tctcatgaac taaaaagttc actttgagat
    21241 actctctttt tttaattaat atacacatta attactatct tagtttatta ctatatatca
    21301 attactactc attaatcctt gtatatatta attcttatat atcatttata gttaggttat
    21361 aattttatgt tatttacatt aatcccataa cagtgagtaa aactcttact atcaacatcc
    21421 ccactattgc atcaaatata ggaactgcca acagtaacat tctatactta gtactggcat
    21481 ttgctacaag cactattctg gcataatgac caactagagt acccattaac atacatggca
    21541 taattaatat ggttgcttgt ggtgcagtga ttagaccttg tgcaaataag tttgcagctg
    21601 tagctgctcc tgctgcctta gcaaagaatg ctgctatcaa aactacaaca gattccccag
    21661 gtagtccaaa aatacccatt actggtccaa aaatatttcc taaaatatca actaaaccag
    21721 ttaattgtaa aaactgcact attacataac ccattatcat ggctggtaat atctgttcaa
    21781 cacctatgta aaaacctttt tttgctcccc ccataaatat ctcaacagca gttttcttag
    21841 gtttactatt tacaattaca tttccagtat tttgttgact catactacac agcctcctta
    21901 gccttattct ttttatttct tacacctatg attaatctaa ctagattagc accaagtatt
    21961 ttacagaata tctctataag tattattgga cctaatgcca ataatgctat tggtaaaagt
    22021 ggagcttgag tatttattgt gttcagtata acagctgaac cagcatattg atatgctaca
    22081 aatatagttc tttcatcatc tgttatttcc ccactttcaa ataattcttt cgtcattatt
    22141 gaggctacat ctgaactagt aaatgaagat acaaatgcta ttcctgctat tccaggtata
    22201 ccaagcagag gtctaagcaa tggattaaat atctttgatg ccgctttcat tcctcccata
    22261 gattcaacta catctataag acctacagca aaacagacag ttggaatcaa agtcagagca
    22321 aataagaaac catcttttgc tcccgttcct cctttaccca taaagtctac accatctgca
    22381 atagtaccaa atgaacctgt cagattagca aaatctaatg ctttaaggaa gccttgacta
    22441 tctttaaaca ctcctgaaaa taatactatc aatacaaata aagataaata ccctttccat
    22501 gttaccttat tatctccagc tgttacatcc atataaaatc cccctttata cctcaaattt
    22561 atctaagttt aaaataactg tagaaattaa tttcaccctc tcaaacatag aatctactac
    22621 tgcatattct cttgatgtgt ggttccattc tcctcttaca ccaaatgaac agattgtagg
    22681 aacgtttgca atagttaaat aacttgcatc tgaactacct ccaagtctct tagcatttaa
    22741 ttctccaaat ccattttctt ttgacacttc atttacaaat gtatgaaatc tcataacatc
    22801 ttctgtagtt tcaaatggca tcatttcact tataaaatta acttctgtag aagttccttc
    22861 tatgtaagtt tctttacata tctctctaac ttgttcttta acattctcca tttcttctac
    22921 tttttcaaat ctaaggtcta cttttatact acaatcttct ggaatagaat tttccactct
    22981 tccaccctta atcacactta cacttactgt agttccaaca tctaaattag tcaatttttg
    23041 tatgcgtagt atcttattag ccatttcttc tatagcattt cttccacctt caaaatcatt
    23101 acctgcgtgt gtttctacac cttttacatg tatattacat cctattcttc cttttctacc
    23161 tacacacaaa ctattatcta ctaatccagt ttccatatta aaagcacaaa gaccaccttt
    23221 tgcttccctc aatataacat ctgcacctgt tgaatcctta tgtcctatct cttcatctcc
    23281 agaaaatact atttttattg gtctttcttt gtatcctatt ttatttaatg ctttaatagc
    23341 atataaggaa attattatac cacccttcat atctaaaaca cctgggccat atgcttttcc
    23401 ttctattatt ttaaatggat tctctccaaa tgtacctgtt tcaaagacag tatccatgtg
    23461 acctgaaaaa attattggct ttttatctat attggaacca agtgtaccta tcaatgtact
    23521 accattatct ccaacatcta ctaactcgca atctaaacct tctttttcaa actcaaactt
    23581 taatctctta gccaatttat ttacgctttc tttacaatgt gtatagcttt ccatattaac
    23641 aatctctttc cataatgaaa ctatttcttc tctattctca tcaacaaact tagaaatttc
    23701 atttaaatat gtattcataa tcttctcctt tattttcaaa taaaatgtat ttctttccta
    23761 aaatctaagt ttattttagg aaatcttaaa agactagtcc ttaattacaa aatagcacaa
    23821 ttattcttga cagtcaacat atttttcagt cttttttata ttttctgatt tttctttcaa
    23881 ttttcctttt gtaaaataaa ttgacgcgtt ttatctacta ttataaaatg aggttataaa
    23941 ttgattgatt agttacaata ctctcgaaat tttaaaggag gttttatgaa atataaaatt
    24001 ggttttttga caacatctta ttttatgtca gataagttta aacaagctct taaagaatta
    24061 aatgatactt gtgaaattac atttattgag cttaaagggg agaatccttt taataattta
    24121 cctaatatat actatgagca agtagataat tttgatggat ttgtatgcag tggtatagtt
    24181 ccatatagtg aacttataat aaatataaaa gacataaaag ttccattaaa ttttttaaag
    24241 cttgatgaaa aagattttta taaatactta ttcaaattat taaatagcca aaaagatatt
    24301 gactttacaa aatcatttat ggatttttta agagaggaca acaattatta tgatatatat
    24361 tcattaatag actctaataa atgcccatat acaattaaag attttaatat ccctcaacct
    24421 gtatatgact ttaaaaaatt aaatgataaa ttattggaaa tacatttaga cttattgcga
    24481 gataataaga ttaacctttc ttttactaga aattacatca ttagttgtga acttaataaa
    24541 cttggatatg attctattta catggttcca tctacagaat caatcctgaa tacatttaaa
    24601 tctctggtga atgaaataac attacaaaac ttagacaaaa ataaatctgc aacttgtata
    24661 gttacagtaa atagttcaga gtacataaat gaagatttga tatttaaaaa tgatgagata
    24721 caaaatcaaa tatataacac tatattaaat tctctatcaa gacatggatt ctatggagtt
    24781 caggtcaaaa aaaatgatat gaaaatagag atacacacta ctaaagaaat gttaaacaat
    24841 atgaccaata actatacaga tttttttatt tcagaagatt taaaagaaat taaatatagc
    24901 ttaaatatag gttggggcat tggaaataca aatattcatg cagagcaaaa tgctcgtcaa
    24961 gctaatggta aatctgcttc tttgaatgga aactgtactt ttatagtaaa tgatactaat
    25021 gatacaatag gccctttata ttcaaataaa aattctaata atattgaaga tagctcaata
    25081 gccaataaag ttgcttcttt tatacctcta tctaatacaa atgtatctaa aatcatgtgt
    25141 atgattaatg atagaaattc aaataatgtg tcagcagaaa tattagcaga ctatatgaac
    25201 ataactctta gaagtgctaa tagaatattg agtatcttat ataaagcagg aattgcaact
    25261 attgtaaata caaaactcga taatcaaaga ggaaggccaa aaaaaatata taaagttgat
    25321 ttcttatctt ttctaaataa gatgcagaaa ttaaattctt aatatttaat tttttaataa
    25381 aagcaatgta ttttctctaa ttttaaagaa tcaaaatgtt taaattttat tagaatatac
    25441 aactttttac tttaaaatac ctaaaccttt tatcagtgaa ataaaccata atatatcgta
    25501 acttattaga aaaacaaaat aatttcatat acaaataata aaatagccac tacaatccat
    25561 aatttgcagt ggctactttt tacactatca cttattactt atttttaaaa attaattgta
    25621 ttttttaaat aatttataca cacctattat cagtaaagca ataaatacaa gtatatctat
    25681 aaaaataatt attgtaacat gtctgacaat attgtaaata aaaaatatag agaatatttc
    25741 tatagacaac gctacaaaac acataaaatt cgttaaataa tctgcaatag tatcactata
    25801 aaaagcatgt atttgtaacc atctccatat aaatggacgt aaaagtattc tgaatacaac
    25861 acaaatgaca gggtaggcat aaataaattt tttatctaca taattagcta caccattatt
    25921 ccattggata ggaatttgag gtggcaactt ttgaaatact gttattgcaa tttaaacact
    25981 acctctctat tctattaatt tataactata atatagtctt aaatattttc actctataaa
    26041 attgtatatt tagtatatct taaaatcaaa ctaattatct tttataagtt ttcat
//
LOCUS       DAMJYR010000045        26001 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.045, whole genome shotgun sequence.
ACCESSION   DAMJYR010000045 DAMJYR010000000
VERSION     DAMJYR010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 26001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26001
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.045"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            118..1032
                     /locus_tag="PC471_001849"
     CDS             118..1032
                     /locus_tag="PC471_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParM/StbA family protein"
                     /protein_id="HDF2788980.1"
                     /translation="MEENYQVIGLDIGRGYVKGYSKYNGMVKECLFKSVFGDGRNIDF
                     EKYENPIYIDFESVSYFVGSLAEKESITPIRNSDDSKVSFTMRILVAAALNEIAVADE
                     VKLMMGVPYKSFRKTTLKEVVDTYKGKTFKVKDKIKGGHKEIKISDISIFREGDAALF
                     HTLEGKVNEDKAVGMVSIGFRSTEMSFFEKGFVFNDKLSDTLEAGNQDALTMVQKQLK
                     DRGIIRELNEIDSSNDYDELKKVAYIMASESTAQRISSKWKNIDEMDVYVSGGTALHM
                     TFDNRFKVSKDAQMATAKGLFEVGMEQF"
     gene            1045..1317
                     /locus_tag="PC471_001850"
     CDS             1045..1317
                     /locus_tag="PC471_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2788981.1"
                     /translation="MKKSSSFYLEEDIFGEIEKYQNDKNISSRNTALERIILEWKNLQ
                     EENKLLKQCLGNGVYRTNKKTNVTEEKKDIKRENPIIKNIFNNMPD"
     gene            1589..2452
                     /locus_tag="PC471_001851"
     CDS             1589..2452
                     /locus_tag="PC471_001851"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2788982.1"
                     /translation="MKRGEVLEIGKRKKSFSPEQRAIINNKKELPYHNFKLGGFVFCT
                     YVKNELLFNEVNISKANISRLIYLATYLNYNTGEENLLVRYSQFKEMIPIDRKNMREI
                     LNLKDRAFRNFLADVKKCELLYESNNKFYINPKYFTKGIPNFENKEYTRIYINTSRFL
                     FEHCTPRQHKQLSYIYQLIPYIHFESNILCLNPHETDISKIKKISLYKICQLLNISTI
                     KQDMNKFKKELLRFYIKVEDDKYYFFKYVIVEGNNKNTDYFIINPCVIWKGNNLNLAK
                     KTIDLCFFNCI"
     gene            2630..2878
                     /locus_tag="PC471_001852"
     CDS             2630..2878
                     /locus_tag="PC471_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2788983.1"
                     /translation="MKEKKIELIYLEDLDESEKTGVHKNRYQILNEEYEQMIDTYTEM
                     SKRSSYDIVNERHKEDFKDELWDYDDTDYDDGLQGKEV"
     gene            2881..4323
                     /locus_tag="PC471_001853"
     CDS             2881..4323
                     /locus_tag="PC471_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2788984.1"
                     /translation="MIYNNNNFYEEIDGKSAQDYIKNLDYSIDNSKDRIKYIEERLGV
                     KHTWFEESNRNYIDTFYNTLTKRNENIITRVKYQEKKQFNDKFWENIFEQTSDSPLDK
                     DGVYYVTIDGEEVVMDYNRFITWCGYNQINPIEYINVKNPFSNSNGTWEYTHHNTSKI
                     KLILNKDDSIYSTSNIAKTLEIMGSYILSVDDKPKETKIRIYNSKELFNRACQEEALL
                     NKIAPANGGNINSNTSNKDGFLEDNSFAFFQLPKNYKKIKDIKVKPKDIKKYPIIKTY
                     SDTYEWYKAKYKELGHKQLSKEELKLKRMVRKNLNALKNDMTDVKNSIERPIIWKAPL
                     ADAGSPEWDYLDMFDKSHVKELLRIQKGNDLQDDLTCIVMDLNNIISKIEFTTVQKDI
                     LNLYRKDKSLEFISNLMNITPQAINNQINKIVNKIIDAYEKNYEENYYYISVCKGKYK
                     KCSRCGKIKLLSRFDKNGKKGYRSNCKNCN"
     gene            4457..4735
                     /locus_tag="PC471_001854"
     CDS             4457..4735
                     /locus_tag="PC471_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HDF2788985.1"
                     /translation="MKKVDFLKEVAEELGVSQIRVREILDVIEKKRNEVLQDGEELTL
                     MGVKYLTKIQKGKEGEITLKDGTKKPWKTEEKRVPKLKIAKSLKDAII"
     gene            5351..5441
                     /locus_tag="PC471_001855"
     tRNA            5351..5441
                     /locus_tag="PC471_001855"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5384..5386,aa:Ser,seq:gct)
     gene            5497..6114
                     /locus_tag="PC471_001856"
     CDS             5497..6114
                     /locus_tag="PC471_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2788986.1"
                     /translation="MVKYNVHKALAEKKLLEDKIDRCISNFKIVGTKKGSDKNVYETK
                     TSVEDFNVEVSSKYQQIEDLIYNYNALDKAINISNAITNVQISNKNYTVLEAIKRKNS
                     IELDKSLLRQMVSNYDCMMSEVNRRNEEVQKNTDRMFEEKEKSKDGAELISFYKKQQE
                     WSLVDPLKVREKIEKLRDEIEEFEKEVDFALSTSNALTIIDVDLR"
     gene            6484..6672
                     /locus_tag="PC471_001857"
     CDS             6484..6672
                     /locus_tag="PC471_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009900721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2788987.1"
                     /translation="MKNNKGTVLEKCEIIKQENNTYKIIEHLKDGDHEVLLSDVLDKY
                     TGDTDLNITIRLSETIVQ"
     gene            6749..7948
                     /locus_tag="PC471_001858"
     CDS             6749..7948
                     /locus_tag="PC471_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2788988.1"
                     /translation="MEDILKKKEDESLVDYRIRIRLAKVNKEIDLDWGEIVELLGLEC
                     SPDHCRKVSYGLKEAFDYLNSKVQDNSTQEEIDKINEKILELKKIKVQLSDERSLVNK
                     KIREYSRIDNIIDLFNNKIDDISLHKPFLSDSSYKCYESSNQEAIMLISDIHYGLETI
                     NAFNRYNSEIFKIRIQYLKDKIIEYSKLHKVKRLHVMLLGDLISGYIHNSIRLENREN
                     IVEQIIEVSEILSEFIYELSKEIDKIIVYSIGGNHDRVLPKKDENLDKDNFTLLIDEY
                     IKLRIKNINNVIFQKNIYDNDIIVAKICGNTCFGVHGDKDKMSTAIPKLTSLIKLIPD
                     YIFMAHLHNCKEDNYGESEICVNGSFSGTDSYAKNLRLSSHAMQKLMIFNESGRICTY
                     NINLSKL"
     gene            8079..8978
                     /locus_tag="PC471_001859"
     CDS             8079..8978
                     /locus_tag="PC471_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2788989.1"
                     /translation="MAISKTKCLNCGEEKYTDRYFWKSHSEIFTLNKRFPVCKECFRA
                     RFLLLNGCYNGELVKALKHICFNFDVYFDEKLAKELADKKNKDELIDEYMKIINRNSK
                     YKGKTSLDNLLKEIYTGNNNDKNIVINDEIKLKWGRGFDDYEYKILERKYKEYEEYYE
                     PESLTERKLFEEICIIELEKDKSREKGDMKAFNDLSKLVSSKMQDAEIKPSQKKKAGD
                     SQDDTFGMKMMIYEKNQPVKDRLKEYEDVDGFEAYVNKHMKKPLAVALGLATGKYSIN
                     DGDKDIKFKDDVRDILEGNKNED"
     gene            8968..10716
                     /locus_tag="PC471_001860"
     CDS             8968..10716
                     /locus_tag="PC471_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2788990.1"
                     /translation="MRTKYEKRELSSNDSSRNLLESIGEYWGAFYLANPHRFCMDYFG
                     FNLHLFQQILIYMMMKSDQFVFIASRGLGKSWLLGVFCCVIAVLKPGTCVLIAAKRKK
                     QAKLLITSKILGDLYLKSDTLKREIKSFQVNAQEVSIDFWNGSRIEAVVSNDDARGYR
                     ANVLIVDEYRMVDEGTVNDVLVPFLTNPRQPGYLQNPKYRYMQEENKEIYLSSGWYSQ
                     HWSYKKFMETVKGMLSGEDMFACSIPFTCSLEHGLLTKKRILKEMKKESMSDASFMME
                     YCGVFYNESDDAFFKSSWVNPCRVLESMFYPPSDIEYLENKKKRDKKYHLNKIKGEIR
                     IIGADIALARGVKNDNSIYTLMRMLPNEGTYKRCVVHIEAYNGMEAEKQAIRLKQLFS
                     DFQADYMILDTQGIGTTVWSYIQKANYDSDRDEWYDAYTCFNEDNTVDKSLAKKSLPV
                     VYSMKAYADENHKMAMSLRDVLTNRTLELPISDIEAKEMILEKEMIKADEIDKKAELE
                     AKYIAPYLQTTALVNELINLEYSADGGKIKIKEKSGARKDRYSSLAYTNFLADYLEEK
                     EKRKNRNNQKTVMIYW"
     gene            10733..12211
                     /locus_tag="PC471_001861"
     CDS             10733..12211
                     /locus_tag="PC471_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2788991.1"
                     /translation="MGKKNNNKSKSKDNNSTQNQINMLNAQIGKYASVKESTTLATEM
                     YKLRSISRDKLRKALSNPYQISNTDILQDASMILKATSGTYRRVLNLISNMNTFDHIL
                     YPKDISRLKTKEKITKAYMNSASQLEKYNIKFTSAWITEKVLELGEVYLYKIEDNSGM
                     VLQQIPAKFCMITMIENNVMRYAINIKKLTDKNIISFPEEIKNIYKKYKSNLLTREEL
                     IDNTYFQLSDKAVAFNYDLDSVKGVPFFCFIFDDLMELEDMKDLKSTNAVIESIKLIH
                     QKIPFGKNDEPLVDLNLIPIYHNSTKANLPKGTAITTNPLELETHTLSDGKSKINDYV
                     KEAKEFIFDNAGINTALLNSDKINNESILNGIIADSLIPMRIQQMIENWVNFELNKDK
                     KANLFNMKFIGTTHFNKMNLSKQYREDMGYGGSKSLFIASTGFTPLQAINTLQAEKLM
                     GFDEFLIPQQTSHTQSSGRPDKSDIGTDNGNSTQAKGNGEND"
     gene            12214..12405
                     /locus_tag="PC471_001862"
     CDS             12214..12405
                     /locus_tag="PC471_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2788992.1"
                     /translation="MSKFIYAFSEDDKKLLMEKGYRFICENKLNNKTLYVFENKSKLI
                     NNFSNEEMKRFIFTSKIRF"
     gene            12412..13845
                     /locus_tag="PC471_001863"
     CDS             12412..13845
                     /locus_tag="PC471_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2788993.1"
                     /translation="MKKIKILKLPCNLKHYSDKKKLEELNSNLLPVYIYVMHEGTNPN
                     GTKFYEEAIDKAEPTLKNVPILGYVKINEDGKYDFDGHNVLTQVVQTDEGFILEEYYE
                     ERIIGVIPETNKYEKVEIDGQKYVKCKGYIYKSYSNHAYDIIMDSDEIEISMEIDIND
                     YQLDDSDGFYNIKDYVYHGITCLGSDVKGAMGSNCCLTKFSRKINYKEEISKICSEIY
                     ALEHGEGEKNLPNKNQKLNKNSEGYALAVSNLSTEIRNKLKEYKVETENWYGEKVEVQ
                     AFYYNDLIPEENIAIVEDEINWGYYYGIPYIVNEDAVILDYENKKSYIQTWREKQEGE
                     IVQVFSRQEKLKKEIIEKFTEKQKEISNLKASLEPLQAFKEEKEFELFKSKVDDVAQK
                     FKLAEDEIKDIKIKAYNKEITLDEYKKELGYIFALKTLSNKQSEKENFSINDDKNNTI
                     KIPISNNNDSFSEPEEISFIKKYSDKE"
     gene            13860..14357
                     /locus_tag="PC471_001864"
     CDS             13860..14357
                     /locus_tag="PC471_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2788994.1"
                     /translation="MKKDKAIIQTDKVKNPDVLTGKYVVPSEDTNPINLENGAVINIG
                     ELEDTEYGRDTHKIYKVTNDTLDWGIVDDPATMYDERLDERDYEVSPGQICRCRRLKK
                     GDEVTISLLHIADKSIAVKDKLQLKSDSFQFEKLPTADAKTPVAEVLELWNYEGQDSV
                     TIKVL"
     gene            14445..15455
                     /locus_tag="PC471_001865"
     CDS             14445..15455
                     /locus_tag="PC471_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2788995.1"
                     /translation="MATAKTLKRIAHELYTDTFREYTDRDGNTITYRDAESAIKNKLK
                     EMMGGEKYNYYKFQENKWGFYNLISELISDDINRLNEEVFSPFCDFENFDLGDKKEFT
                     VENTNLFKVANIADGVNSTRRQRLLNKKVPTTAFKLSVAIYEEAERFITGRINWVVFV
                     NRVSDSYHYDIARRIAKTFEGAYSTINAKFQATGNSDKVLLELIAKVEGATGKKPIIY
                     GTPLALSNLEGIQTDLEKEEKRKYGFIQTFRGGYKVLSLPNAYDENAAEGKEWALDNK
                     AIYVIPDGEKIIKLGTEGDVLVIENTDEKERDDQQIEYFMAQKIHLGVVTAAKFGVYK
                     IQ"
     gene            15508..16140
                     /locus_tag="PC471_001866"
     CDS             15508..16140
                     /locus_tag="PC471_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2788996.1"
                     /translation="MARAKKENISKTEVKANTLEKDIKSTLNKKEARLNRLELQRKLK
                     QKKQEIDIEIKNISTWDVDYIDPREKQQIFSLSKVGSKDSTEFIDLDTLYRIVRRSPG
                     FFEEHRLIISDVDSLDVECTPMDIIDFLGLNSLYEHIRNPNEDYLEYFLSDKVDINSF
                     EKILNKNNVELTRRLAERAIDLHKKKKFDSGLKAKLLAKRIGIEDLYLFS"
     gene            16155..16784
                     /locus_tag="PC471_001867"
     CDS             16155..16784
                     /locus_tag="PC471_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2788997.1"
                     /translation="MGTPLVKIYKKFLDAINDEEMLLLSNEIIEKMMYSYLEDAIVDF
                     NQCKKDLTIKYVDEKGEEIIPAAQLSYTSNYSNKNAEITLMGKETKEEYELDKDYTIS
                     FEDEKFIISFVVETTEEIIFKYKYLGEIVSDLDLEEIKILSYGMQIHWLQPKINREEN
                     LKQMLTDSDYNAKSGANMLAKLQAREEQLRTRFNKYQQRYMLKNFEGWN"
     gene            16786..17775
                     /locus_tag="PC471_001868"
     CDS             16786..17775
                     /locus_tag="PC471_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2788998.1"
                     /translation="MSYLSDIKKRIGLGCSTPKEKRILQLRLSFKKYLKETPTCIEVP
                     ITDIDEVCINEDTKKAVVAINDITNNDKRALDEKNLLVESDLDVDVGCYLFYDNCYWL
                     TIFKEHKEMDTYKHFIIKRCNQFFNYKYKGQMYKIPIAVENLTLYSDGMADNKYTSIS
                     DTKRQLYFGSNPVTKTIDIDTRIMLTGKTVFRVTSINDFEYNGRETGADGLIKAICLQ
                     DALISKDDTINNIAWNDLSENDSVIIPFSKIIGYEVINLGEENEYIIDHFQGVKWLLD
                     KQYGYCDIIYQDEKRCVIQANTLAKYSGLEALLLAKDKDTNEIIDTKKITLRG"
     gene            17790..18284
                     /locus_tag="PC471_001869"
     CDS             17790..18284
                     /locus_tag="PC471_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009900745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2788999.1"
                     /translation="MAFPNKMISNIGTTLMSNQDFAKFMIYNNESQKDILSMPDIKNP
                     VKELRNKKVFFNRRVEKVLKEADVSVYITLFKYNLYKDRSRIIKTTNIEIGVVCHDEC
                     QDTFNGLRDAALVCCIVDTITENEEIAGIGKIKLESCSPMFNLDTSYNGFSIVVSSES
                     FGDM"
     gene            18284..19087
                     /locus_tag="PC471_001870"
     CDS             18284..19087
                     /locus_tag="PC471_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2789000.1"
                     /translation="MLENYYITGLPIKLSEILGTIYQPTIEELIKFDMANLEIVNPFL
                     VLEKSYSQLCNEKTFELKCKYDAIPILDLMMLTSRKDSSEEIELLSDKIKKSLSILYK
                     TDIKNIEYMNKIKIGILIKFDDKKKNAFISREDFGLVSDLILEMFCIDKKNLFKDDED
                     KWIENTGSEREKQLIAHFKEKERKKREKEAYHLCDYINVVQNIDGYVPIDVILKMTYW
                     QLINVYKTKIQFKNYNESLGYAWSFKYQTDMDKMKHWSKEIKIPLSTVK"
     gene            19132..19950
                     /locus_tag="PC471_001871"
     CDS             19132..19950
                     /locus_tag="PC471_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2789001.1"
                     /translation="MKRFAVKDAGNVIVKNKVTGEVLFYSQDLNAFNFKMDSESVYAK
                     AKGANTIAFDGAITASLTMEQEVIQMAQLAMLLSSDIDEKTAKVGKRKVLTSDSTKKV
                     TLENIKPVANSISVYSIESDGISIIKKLQFTSSVTGANTEITISTADFNAGDKVAVFY
                     LEEIPKAKVVKIKEESTAPNYVVEAEVMVKTVDGEYMVLYMSVPNAKAQRSIELNLTA
                     ENPSGFNMTLDVLPDENKEYATFTFIGDENLNPARMASMLGVELEDEKDTKPKK"
     gene            20049..20834
                     /locus_tag="PC471_001872"
     CDS             20049..20834
                     /locus_tag="PC471_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009900751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2789002.1"
                     /translation="MVFLDDKFLFDNVSSESMNIKLVTLGDDEILNEYGLPYEEAIKS
                     DSSFNKNPCYSEDETTIEPITLQFCLCSEHGEAYEWDDYTLENICNWFWQREFKPFIS
                     YDNIEEIYYFKARKIIKRYTKDKKGVLEIEFQPYTNYAYRSFQKVITVKDTREIKLNN
                     VSNVDEEYAPVIDIECLKEGDITIRNSIISDNEEDNLVISGLELNEKITIDNLYYTVL
                     NSNGENRFNIINRKWIRLRRGVNILKFTGNCKVSIKCKYPIIK"
     gene            20848..21297
                     /locus_tag="PC471_001873"
     CDS             20848..21297
                     /locus_tag="PC471_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009900754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2789003.1"
                     /translation="MNKIQVDKLIQDEVRAIIPIIDENGKEEYIEVRNPDKETKEEIL
                     NKIWVGMENPDLALSQEDILKMLIDKLTNIELNIEIEDLVDSNISSELETVMYHICQI
                     ENELTASLLMNTDVKLGQMKNEILQDRVLKETEEIEKMNNIKDKVVN"
     gene            21298..21738
                     /locus_tag="PC471_001874"
     CDS             21298..21738
                     /locus_tag="PC471_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2789004.1"
                     /translation="MVFKSLDELVAYTKNKIAASMPEVGKEMKEIMKEEVNKQVYSGY
                     TPVGEWNPNVWGGRTGQLLDAIDVTELSSNSVTSEIQNNGNWVDAFTRNSAFPMERLE
                     KGKVWGVGGYRPQTNIMEESNSKAQERVPKCFKSKMNELGVPVK"
     gene            23189..>26001
                     /locus_tag="PC471_001875"
     CDS             23189..>26001
                     /locus_tag="PC471_001875"
                     /inference="COORDINATES: protein
                     motif:HMM:NF014442.1,HMM:NF024352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HDF2789005.1"
                     /translation="MEKYLFNQKLLKEKYNMEIDLDLYDYKQRRLILEKWRDVIDNKS
                     IDKLNEIQLKEIFVSDILNKVLRYKSLTDNIMDYNIKPEEKTKIDGTRADIVLGYFSN
                     EESDYRVAIELKKPTTNLDAKQLRKNNHNTPVEQGFSYLPKYGRNCTWLIVSNFKEIR
                     LYNANDATEYEFFSIKDIYEKDDVFKKFLYLLSSKSLMTKHGKSRVEVLWEENIKQEE
                     AIEKGFYDLYKNTRLNLFNDILLNNKDILDVKTCLTKTQKILDRVIFVCFAEHKGLIP
                     ENIFETCIQVGEKSVSRFGVWEQVKGLFESIDLGNQEKGINQFNGGLFAEDYILNNLK
                     IPNKCFENLYKLSLYDFNSDLNENILGHIFEKSISDLEELKLEINQVNIKKKDSKRNK
                     DGIFYTPKYITKHIVNNTIRRYLDEKKKELGEDKLPELKEEDYETVVVGRNIQHKRVQ
                     VVEYKTDKIQKYINFWKNYRDVVKNIKILDPACGSGAFLNEAFSMLKKEAESVNEILR
                     DLTDGEISFFDLDANILKNNLYGVDLNEESVEITKLSLWLKTANKYSLLTSLDNNIKC
                     GNSIIDNKEVDCAKAFDWNVEFEDVMMSGGFDIIIGNPPYVSTKMIPEKHREYYWKKY
                     KDLLISEMDLYELFLYEFCENKLKQDGFLGFITPNTYFTNKSFENLRKYLLKNVCVDT
                     ILDFPYRFFPFEDVNKETAIIIINKKLPNIKNNINLISINKENMKKIKIFNSNTYNSE
                     NKITIKSIINDLDNKIVIQSNPIILKMLKCKHKLGDYLNLHKGWMSVPNKTICDGIIY
                     DKKILSSNDIKDNNQLNGILNKCLEGKDIHRYYIDKVDKFVNIENMDDKTMRWHKSPK
                     IITQRIVGQNQNKIIATVDIEGTIIFPSGNIINLIKEDNLEDIYFYIGVINSSLINYF
                     YNKFYGESNTNLTADAVKNIPIP"
BASE COUNT        11106 a         2413 c         4513 g         7969 t
ORIGIN      
        1 taactaataa ctaaaaaatg tcattttata gttgaatttt tgtgcaaatt taatgtaatt
       61 atcaagtttt tatatggtat aatatattta gcaaatattt acttaggagg aatgaaaatg
      121 gaagaaaatt accaagttat aggcttagat ataggaagag gatacgtaaa aggatatagt
      181 aagtacaatg gaatggttaa agaatgttta ttcaaatcag tatttggtga tggaagaaac
      241 atagattttg agaaatatga aaatccaata tacatagatt ttgaaagtgt aagttacttt
      301 gttggttcat tagcagaaaa agaaagtata acacctataa gaaattctga tgattctaaa
      361 gtatctttta caatgagaat attagttgct gctgctctta atgaaatagc tgtagctgat
      421 gaagtaaaat tgatgatggg cgttccatat aaatcattta ggaaaacaac attaaaagaa
      481 gttgtagaca catataaagg taaaacattt aaagtaaaag ataaaataaa aggtggacat
      541 aaagaaataa aaatatctga tatatcaatt tttagagaag gagatgctgc attatttcat
      601 acattagaag gaaaagtaaa tgaagataaa gcggttggca tggtttcaat aggttttaga
      661 agtactgaga tgtctttctt tgaaaaaggt tttgtattta atgataaact atcagacaca
      721 ttagaagctg gaaatcaaga tgctttaaca atggttcaga agcaactaaa agatagagga
      781 ataataagag aacttaatga gatagatagt tctaatgatt atgatgaact taagaaagtt
      841 gcttacatta tggcttctga aagcacagct caaagaatat cttctaaatg gaagaacata
      901 gatgaaatgg atgtatatgt aagtggaggt acagcattac atatgacatt cgataataga
      961 tttaaagttt ctaaagatgc acaaatggct acagctaaag gattatttga agtaggtatg
     1021 gaacaattct aggagtgata ttatatgaaa aaatcatcta gcttttattt agaagaagat
     1081 atatttggtg aaatagaaaa gtatcaaaat gataaaaata taagcagtag aaatacagct
     1141 ttggaaagaa taatattaga gtggaaaaat cttcaagaag aaaataaatt attaaaacaa
     1201 tgtttaggca atggtgtata taggacaaat aaaaagacaa atgttactga agaaaaaaaa
     1261 gatataaaaa gagaaaatcc tattattaag aatatattta ataatatgcc agattaaaag
     1321 aaaatatcta ctcaagaaat tttgggtaga tattaaaaaa aatattgaga acatagaggg
     1381 tatatgatat aatagaaata gaacatagag ggtaatgaaa tttctgaagc aacatgttgc
     1441 gaagaacaat tgcaagatga tttttatctt gtaattgaaa taaaatttat aagaaaagag
     1501 gtagatattt atagttagag gtaatgaatt tataaaagat gttattgtaa cagacaaaag
     1561 aacaggagaa atattggagc ataaagggat gaaaagaggt gaggtacttg aaattggaaa
     1621 gagaaaaaag agtttttctc cagagcaaag ggctattatc aataataaaa aagaattacc
     1681 ataccataat tttaaattag gaggttttgt attttgtact tatgttaaaa atgaattatt
     1741 atttaatgaa gtaaatataa gtaaagctaa tataagtaga ttaatatatc tcgcaacata
     1801 tctaaactat aatacaggag aagaaaattt attggttaga tattctcagt ttaaagaaat
     1861 gattcctata gatagaaaaa acatgagaga aatattaaat cttaaagata gggcatttag
     1921 aaatttttta gcagacgtaa aaaaatgtga gttattatat gaatctaata ataaatttta
     1981 tataaatcca aagtatttta ctaaaggaat tcctaatttt gaaaataaag aatatacaag
     2041 aatatatata aatactagta gatttctttt tgaacattgc acaccaagac aacataaaca
     2101 attaagttat atatatcaac tcataccata tatacatttc gagagtaata tcttatgttt
     2161 aaaccctcat gaaacagata tttcaaaaat aaaaaaaatt agtttatata aaatttgtca
     2221 attattaaat attagtacaa taaaacaaga tatgaacaaa tttaaaaagg aattacttag
     2281 attttatata aaggtagaag atgataaata ttattttttt aaatatgtaa tcgttgaagg
     2341 gaataacaaa aatacagact atttcattat aaatccttgt gtaatatgga agggaaataa
     2401 tttaaattta gcaaagaaaa caattgattt atgctttttt aattgtattt agaaagtctg
     2461 tctaagaata agaggtaatg aactgtaaaa attaacaaaa atataggaaa aacagttaaa
     2521 aaattaaatt taggggtggc acagaaatgt gaccaaaagg caaaaaaacg gcacagaaat
     2581 gtgaccaaga aaattaattt aaaatcttat aatatagaaa aggagaagta tgaaagagaa
     2641 aaaaatagaa ttaatatatt tagaagattt agatgaatca gagaaaacag gcgtacataa
     2701 aaataggtat caaatattaa atgaggaata tgaacaaatg atagatacat acacagagat
     2761 gagtaaaagg tcttcatatg atattgtaaa tgaaaggcat aaggaggatt ttaaggatga
     2821 actatgggat tatgatgata cagattatga tgatggattg caaggaaagg aggtataata
     2881 ttgatatata ataataacaa tttttatgaa gaaatagatg gcaaatctgc acaagattac
     2941 ataaagaatt tagattatag catagacaat agcaaagata gaattaaata catagaggaa
     3001 cgattagggg taaaacacac gtggtttgaa gagtcaaata gaaattatat agatacattt
     3061 tataatactc ttacaaagag aaatgagaat attattacta gagtgaaata tcaagaaaaa
     3121 aaacaattca atgataaatt ttgggaaaat atatttgagc aaacatcaga tagtccttta
     3181 gataaagatg gtgtatatta tgttactatt gatggagaag aagtagttat ggattataat
     3241 agattcatta cttggtgtgg ctataatcaa attaatccaa tcgaatatat aaatgttaaa
     3301 aatcctttta gtaatagtaa tggtacatgg gagtacacac atcataatac atcgaaaatt
     3361 aagttgatat taaataaaga tgacagtata tattctactt ctaatatagc aaaaacatta
     3421 gaaataatgg ggagctatat actctcagta gatgataaac ctaaggaaac taaaataaga
     3481 atatacaatt caaaagagtt atttaataga gcatgtcagg aagaagcact tctaaataaa
     3541 atagcacctg caaatggtgg aaatataaat tcaaacactt ctaataaaga tggattccta
     3601 gaagacaatt cctttgcatt ttttcaatta cctaaaaact ataaaaaaat aaaagatata
     3661 aaagttaaac ctaaagatat taaaaaatat ccaattataa aaacatactc agatacttat
     3721 gaatggtata aagctaaata taaagaatta ggacataaac aattaagtaa agaagagtta
     3781 aaacttaaga gaatggttag gaaaaattta aatgcattaa aaaatgatat gactgatgtt
     3841 aaaaattcta tagagagacc aattatatgg aaagcaccat tagcagatgc aggaagccca
     3901 gaatgggact atttagatat gtttgataaa tcacatgtta aagagctttt aaggattcaa
     3961 aagggtaatg atttgcaaga tgatttaaca tgtatagtta tggatttaaa taatattata
     4021 tctaaaattg aatttaccac agtacaaaaa gatattttaa atttatatag aaaagataaa
     4081 tcattagagt ttatttcaaa tttaatgaat ataactcctc aagcaataaa taatcaaata
     4141 aataaaatag tgaacaaaat aatagatgca tatgagaaaa actatgaaga aaattattat
     4201 tatatatctg tttgtaaagg aaaatataaa aaatgtagca gatgtggaaa aattaagttg
     4261 ctatctaggt ttgataaaaa tggtaaaaag ggatatcgct caaattgtaa aaactgtaat
     4321 tagctggtgg ggaaaagtgg tttaaaaaag ggtcgcttaa aacatattac ttatgtaagg
     4381 ggttaataaa aataataaaa ttattcctta ctattatcaa atgaaaatta tataagaaaa
     4441 gggagattaa gaagatatga aaaaagtaga ttttttaaaa gaagtagcag aagaattagg
     4501 tgtatcacag ataagagtaa gagagatact tgatgtgata gaaaaaaaaa gaaacgaagt
     4561 actacaagat ggtgaagaat tgacgttgat gggagttaaa tacctaacta aaatacaaaa
     4621 aggtaaagaa ggagaaataa ctcttaaaga tggaactaaa aaaccttgga aaactgagga
     4681 aaaaagagtt ccaaaattaa aaatagctaa atctttaaaa gatgcaatta tatagagagc
     4741 gtatggctta aaagcccttt catatggtga catatggtag caaacctaga atatgctaaa
     4801 aattaataca attagcagag atagctcatt ttgagaagct ctagtggctg gtaataggta
     4861 ttttacaaat ataagatgat gatgctgcta aaatgaatat agcgtggcaa atatacacga
     4921 ggttgacgag caacatataa cttgaaatag atacggtgaa tgccctagta tgccttaaag
     4981 gtgtaagaat aaagaagtag aatttgaggt aacagattct acttctgctt tttaaaatta
     5041 gaggagtttg ttggtgtgcc tggctaataa aaaaccaaaa acaagtcgag gtgtttgtca
     5101 gaagaacctg gctaataaaa aactgaagaa attgtgaata aatcaaatag tcttttgaac
     5161 taagtaattt ttatgtagat ttatttataa aaaagtggtg atgttctacc aaatagtgga
     5221 caattaaaaa agattcttta gtgtgaaaaa acagccacgc tatccgagct gtcggagttt
     5281 taaaatacag cacttgacca tggtcgaggg tagggatact cgacttgctg tttgtaattt
     5341 atagaaatat ggagaggtac tcaagtggta aagaggatag tttgctaaac tattaggtca
     5401 attaatttga tacgagggtt caaatccctt cctctcttcc aataaaaaaa gtttgttatt
     5461 tttaaataat atcttaatta aaagggagat gtttttatgg ttaaatataa tgttcataaa
     5521 gctttagcag aaaagaagtt attagaagat aagatagata gatgcattag taattttaaa
     5581 attgtaggta ctaaaaaagg ctctgataaa aatgtatatg aaacaaaaac aagtgtagaa
     5641 gattttaatg tagaagtttc aagtaaatac caacaaatag aagatttaat atataattat
     5701 aacgcattag ataaagcaat taatatctct aatgctataa caaatgttca aattagtaat
     5761 aaaaattata cagttttaga agcaataaaa agaaaaaata gtatagaatt agataaatcc
     5821 ttgttgagac aaatggttag taactatgat tgtatgatgt ctgaagtaaa tcgtagaaat
     5881 gaagaagtgc aaaaaaatac agacagaatg tttgaggaaa aagaaaaaag taaagatgga
     5941 gcagaactta tttcatttta taaaaaacaa caagaatggt ctttagtaga ccctttaaaa
     6001 gtaagagaga aaatagaaaa attaagggat gaaatagaag aatttgaaaa agaagttgat
     6061 tttgcattga gtacatcaaa tgcacttaca attatagatg tagatttaag atagtcctta
     6121 attggactat tttttaattt gcaggttagg cgaataacat aaattataag ttcccatttg
     6181 gttttgggtt aaaaatcaaa ctgcttatat aaaagtgatg ggtacataca aacgaaacga
     6241 ttgtactgta taaaagagaa caatagatat aaagcttaaa gttaaaaatt caaatataaa
     6301 atctcaagga taaaagatta aattattaaa atagtaaaaa taaaataaaa aagttataga
     6361 aaatccttga taaatgctaa atgcgttaac ttatattagc tattgttagc tacaaggctg
     6421 cctaatttgc aaataaatat attcccagta gagttaataa aattataaaa aaggtgaaag
     6481 tgcatgaaaa ataataaagg aacagtatta gaaaaatgtg aaataataaa acaagaaaat
     6541 aatacatata aaattataga acatttgaaa gatggagacc atgaagtatt acttagtgat
     6601 gttttggata agtatactgg tgatacagat ttaaacataa cgataagatt aagtgaaaca
     6661 atagtccaat aaatcaatat tggactattt attttttact taatacattg ataatgatat
     6721 ttatgtgata aatagggaga tattaaatat ggaagacata ttgaagaaaa aagaagatga
     6781 gtcactagtt gactatagga ttagaatacg actagcaaag gttaataaag agatagattt
     6841 ggattgggga gagatagttg aactattagg gttagaatgt tctccagacc attgtaggaa
     6901 ggtttcttat gggttgaaag aggcttttga ttatcttaat tctaaagtac aagataattc
     6961 tactcaagaa gagattgata aaataaatga gaaaatatta gagctgaaaa agataaaagt
     7021 acagttatct gatgaaaggt cattagtaaa taagaaaata agagaatatt caagaataga
     7081 taatataatt gatttattta ataataaaat agatgatata tcacttcata agccatttct
     7141 aagtgattct agttacaaat gctatgaatc ttcaaatcaa gaggctatta tgctaatttc
     7201 agacatacat tatggattag agactattaa tgcatttaat agatataatt cagaaatatt
     7261 caagataaga atacaatatt taaaagataa aattattgaa tatagtaagt tacataaagt
     7321 taagagactg catgtaatgt tacttggaga tttaatttct ggatacatac ataattctat
     7381 aagattagaa aatagagaaa atatagttga acaaattata gaggtgtctg aaatacttag
     7441 tgaatttata tatgagttat ctaaagaaat tgataaaatt attgtttatt caataggtgg
     7501 aaaccatgat agagttcttc ctaaaaaaga tgaaaattta gataaagata attttacatt
     7561 attaatagat gaatatataa agctaagaat taaaaatata aacaatgtta tatttcaaaa
     7621 aaatatatat gataatgaca taatagtggc taaaatatgt ggaaatactt gttttggagt
     7681 acatggagac aaagataaaa tgtctacagc aattcctaaa ctgacctctc ttataaaatt
     7741 aattcctgat tatatcttta tggctcatct acacaattgt aaggaagata attatggaga
     7801 aagtgaaata tgtgtaaatg gaagtttctc cggaacggat tcttatgcta agaatctaag
     7861 attaagttca cacgcaatgc aaaaattaat gatatttaat gaaagtggga gaatatgtac
     7921 ttacaatata aatttaagta agctataagt tatttattaa gagctgagtt aagtttatat
     7981 tgtaaattta attcagctct taattgagtt gaatctccct attaaccact agagatttaa
     8041 ttctctagtg gtctttttta atggtggtga tgttatgaat ggcaattagt aaaacaaagt
     8101 gtttgaattg tggtgaagaa aaatatacag atagatattt ttggaaaagt catagtgaaa
     8161 tatttacttt aaataaaaga tttccagtat gcaaagaatg ttttagagct aggtttttac
     8221 tattaaatgg gtgttacaat ggagaacttg taaaggcact aaaacatata tgtttcaact
     8281 ttgatgttta ttttgatgaa aaattagcta aagaacttgc agataaaaaa aataaagatg
     8341 aattaataga tgaatatatg aaaattatta atagaaattc aaaatataag ggaaagacat
     8401 ctttagataa tttattgaaa gaaatatata caggaaataa taatgataaa aatatagtca
     8461 taaatgatga aattaaatta aaatggggaa gaggttttga tgattatgag tataaaatct
     8521 tagaaagaaa atataaggaa tatgaagaat attatgaacc agagtcatta actgaaagaa
     8581 aattgtttga agagatttgt attattgaat tagaaaaaga taaatcaaga gaaaagggag
     8641 atatgaaagc ttttaatgat ttatctaaat tagtatcaag taaaatgcaa gatgctgaaa
     8701 taaagcctag tcagaaaaag aaagctggag attcgcaaga tgatacattt ggaatgaaaa
     8761 tgatgatata tgaaaaaaat caacctgtaa aggataggtt aaaggaatat gaagatgtag
     8821 atggatttga agcttatgta aataaacata tgaaaaaacc acttgctgtt gcattaggtt
     8881 tagctacagg taagtattcg ataaatgatg gcgataaaga tattaaattt aaagatgatg
     8941 taagagacat tttagaaggt aataaaaatg aggactaaat atgaaaaaag agagctgtca
     9001 tctaatgatt catcccgaaa tctactagag agtataggag agtattgggg tgctttttat
     9061 ctcgcaaatc cacatagatt ctgtatggat tactttggat ttaatctaca tttatttcaa
     9121 cagatattaa tttatatgat gatgaagtct gaccaatttg tatttattgc ttctcgtgga
     9181 ctgggaaaat catggcttct aggagtgttt tgttgtgtta tagctgtatt aaaaccaggt
     9241 acttgtgttt taatagcagc aaaaagaaag aaacaagcaa aactacttat tacaagtaaa
     9301 attttaggtg atttatattt aaaatcagat actctgaaaa gagaaattaa aagtttccaa
     9361 gtaaatgcac aagaagtatc tatagatttt tggaatggaa gtagaataga agctgttgta
     9421 tctaatgatg atgcaagagg ttacagagct aatgttctta ttgttgatga atatagaatg
     9481 gtggatgaag gaactgtgaa tgatgtttta gttccatttt taacaaaccc aagacaacca
     9541 ggatatttac agaatcctaa atatagatat atgcaagaag agaataaaga aatttatctt
     9601 agttcaggct ggtattctca gcattggagt tataaaaagt ttatggaaac agtaaaaggt
     9661 atgcttagtg gtgaagatat gtttgcatgt agtattccat tcacttgctc tttagaacat
     9721 ggactattaa ctaaaaaaag aatacttaaa gaaatgaaaa aagagagtat gagtgatgct
     9781 tctttcatga tggagtattg tggagtgttt tataatgaat cagatgatgc tttcttcaag
     9841 tcatcttggg tgaatccatg tagggtatta gaaagtatgt tttatcctcc tagtgatata
     9901 gagtatcttg aaaataagaa aaaaagagat aagaaatatc atcttaataa gataaaagga
     9961 gaaatacgga ttattggtgc agatatagct ttggccagag gagttaagaa tgataattct
    10021 atttatactt tgatgaggat gcttccaaat gaaggcacat ataaaagatg tgttgtccat
    10081 atagaagctt ataatggtat ggaagctgaa aaacaagcaa taagattaaa acaattattt
    10141 tcagattttc aggcagatta tatgatatta gatacacaag gtataggaac aactgtatgg
    10201 agctatatac agaaagcaaa ttatgattca gatagagatg aatggtatga tgcttacaca
    10261 tgttttaatg aagataatac tgttgacaag tcactagcaa aaaaatcact tcctgttgta
    10321 tattccatga aagcttacgc tgatgaaaat cataaaatgg ctatgtcttt aagagatgtc
    10381 cttacaaata gaaccttaga acttcctata agtgatattg aagcaaaaga aatgatatta
    10441 gaaaaagaaa tgataaaggc agatgaaata gataaaaagg cagaattaga agcaaaatat
    10501 attgcccctt atcttcaaac aacggcttta gtgaatgagt taataaattt agaatatagt
    10561 gctgatggag gaaaaattaa aataaaggaa aaaagtggag caagaaaaga tagatattct
    10621 agtttggctt atacgaattt cttagcagat tatctggaag aaaaagagaa aagaaaaaat
    10681 agaaataatc aaaaaactgt tatgatttat tggtaagaga tgaggtgaat aagtgggcaa
    10741 aaaaaataat aataaaagca agtctaaaga caacaattct acacaaaatc aaatcaatat
    10801 gctaaatgct caaattggaa aatatgcctc agtaaaagaa tccacaacat tagcaacaga
    10861 aatgtataag ttaagaagta taagcagaga caaattaaga aaagctctct ccaatccata
    10921 tcaaatttca aatacagata tacttcagga tgcaagtatg atattaaaag ctacatctgg
    10981 tacatataga agagttttaa atttaatatc taatatgaat acttttgacc atatcttata
    11041 cccaaaagac ataagtagat taaaaacaaa agaaaaaata acaaaagcat atatgaattc
    11101 ggcgagtcaa ttagaaaaat ataatataaa atttacatca gcttggataa ccgaaaaagt
    11161 attggaatta ggagaggttt atttatataa gatagaagat aattcaggga tggttttaca
    11221 acagatacca gcaaaatttt gtatgataac aatgatagaa aataatgtaa tgagatacgc
    11281 tataaatata aaaaaactta ctgacaaaaa cataatatcc tttccagaag aaattaaaaa
    11341 catatataaa aaatataaat ctaatttact tacacgagaa gaattaattg ataatacata
    11401 ttttcaatta agtgataaag cagtagcatt taattatgat ttagattcag tgaaaggagt
    11461 tccatttttt tgctttatat ttgacgattt aatggagctt gaagatatga aagatttaaa
    11521 aagcacaaat gcagtaatag aaagtattaa attaatacat caaaagatac catttggcaa
    11581 aaatgatgaa ccactagtag accttaattt aattcccata taccacaatt caacaaaagc
    11641 aaatttacca aaaggaacag caataactac aaatccttta gagttggaaa cacatactct
    11701 ttcagatgga aaatctaaaa taaatgatta tgtaaaagaa gcaaaagagt ttatatttga
    11761 taacgctgga ataaatacag ccttacttaa ctctgacaag ataaataatg aatctatctt
    11821 aaatggtatt attgcagata gcttaattcc tatgaggatt caacaaatga tagaaaattg
    11881 ggttaacttt gaactaaata aagacaagaa ggcaaattta tttaatatga agtttattgg
    11941 aactactcac tttaataaaa tgaatttatc caaacagtat cgagaagata tgggatacgg
    12001 agggagtaaa agcttgttta ttgcaagtac tgggtttact ccacttcaag ctataaacac
    12061 tttacaagca gaaaaattaa tgggatttga tgagttttta atacctcaac aaacatctca
    12121 tactcaaagc agtggtagac cagataaatc tgatataggg acagataatg gtaattctac
    12181 tcaagcaaaa ggaaatgggg agaatgatta attatgagta agtttatata tgctttttct
    12241 gaagatgata agaaattatt aatggaaaaa ggatatcgtt ttatatgtga aaacaagttg
    12301 aataataaga ctttatatgt ttttgaaaat aaatcaaaat taataaataa tttcagtaat
    12361 gaagaaatga aaagatttat atttactagc aaaatacgtt tttaaaagga ggtgaaaaaa
    12421 ataaaaatat taaaattacc ttgtaattta aaacattatt cagataaaaa aaaattggaa
    12481 gaactaaata gtaatctttt gccagtgtat atctatgtta tgcatgaagg tacaaatcca
    12541 aatggaacta aattttatga agaagctatt gataaagctg aaccaacatt aaaaaatgtt
    12601 ccaattctag gatatgtaaa aataaatgaa gatggaaaat atgactttga tggtcataat
    12661 gttttaacac aagtagttca aacagatgaa gggtttatat tagaagaata ttatgaagaa
    12721 cgaataattg gcgttatacc agaaacaaat aaatatgaaa aagttgaaat tgatgggcag
    12781 aagtatgtta aatgtaaagg atatatatat aaatcatata gcaatcatgc ttacgatata
    12841 attatggatt ctgatgaaat agaaatttca atggaaatag atataaatga ttatcaatta
    12901 gatgattcag atggatttta taatataaaa gactatgtat atcatggaat tacatgtctt
    12961 ggttctgatg taaagggagc tatgggttca aattgttgtt taactaaatt ttcaagaaaa
    13021 attaattata aagaagaaat atcaaaaata tgctcagaaa tttatgcatt agaacatgga
    13081 gagggggaaa agaatttgcc aaataagaat caaaaactta ataaaaattc tgaaggatat
    13141 gctttagcag tgagtaatct tagtactgaa ataagaaata agttaaaaga atacaaagtc
    13201 gaaactgaga attggtatgg tgaaaaagtt gaagtacaag ctttttatta taatgattta
    13261 ataccagaag agaatattgc tatagttgaa gatgaaatta actgggggta ttactatggt
    13321 attccatata tagttaatga agatgctgtc attttggatt atgaaaataa aaaatcatat
    13381 atacaaactt ggagggaaaa acaagaagga gaaatagtac aggtttttag tagacaagaa
    13441 aaattaaaaa aagaaataat agagaaattt actgaaaagc aaaaagaaat atctaattta
    13501 aaagccagtt tagagccatt acaggcattc aaagaagaaa aagaatttga gttatttaaa
    13561 tctaaagttg atgatgtagc tcaaaaattt aaattagcag aagatgaaat taaagatata
    13621 aaaataaaag cttataataa ggaaattaca ttagatgaat acaagaaaga attgggttac
    13681 atatttgcat taaaaactct tagcaataaa caaagcgaaa aggaaaattt tagtataaat
    13741 gatgataaaa ataatacgat aaaaatacct ataagtaata ataatgatag ttttagtgaa
    13801 ccagaagaaa tatcttttat aaaaaaatat tcagataaag aataatagga ggaatttaca
    13861 tgaagaaaga taaagcaata atacaaacag ataaagtaaa aaatcctgat gttttaacag
    13921 gtaaatatgt tgttccatca gaggatacca atccaataaa tttagaaaat ggtgctgtaa
    13981 taaatattgg agaacttgaa gacacggagt atggtaggga tacccataaa atatataaag
    14041 ttacaaatga cacattagat tggggtatag ttgatgaccc agcaactatg tatgatgaaa
    14101 gattagatga aagagattac gaagtttctc ctggtcagat atgtagatgt agacgactaa
    14161 aaaaaggaga tgaggtaact atatctctat tacatatagc agataaaagt attgcagtaa
    14221 aagacaagtt acaattaaaa tctgattcgt ttcagtttga aaaattgcct acagcagatg
    14281 ctaaaactcc agtagcagaa gtattagaat tatggaacta tgaaggtcaa gattcggtta
    14341 caataaaagt tttataaaat caaataaaat taacaaacag gagataacag tctataactg
    14401 ttattttttt atgcaaaaaa atagaaaagg aagtgtgtaa attaatggca acagcaaaaa
    14461 ctttaaagag aatagctcat gagctatata cagatacatt tagagaatat acagatagag
    14521 atggaaatac aataacatat agagatgctg aaagtgcaat aaagaataaa ttaaaagaaa
    14581 tgatgggagg agaaaaatat aattattata aattccaaga aaataaatgg ggattttata
    14641 atttaatatc agaattaata tctgatgata ttaatagatt aaatgaggaa gtatttagtc
    14701 cattttgtga ttttgagaac tttgatttag gagataaaaa agaatttact gttgaaaata
    14761 ctaatttatt taaagttgca aatatagcag atggtgttaa tagtacaaga agacaaagat
    14821 tacttaacaa aaaagttcct acaacagcat ttaaattatc tgttgctata tatgaagaag
    14881 cagagagatt tataacagga agaataaatt gggttgtatt tgtgaatagg gtatcagatt
    14941 catatcatta tgatattgct agaagaatag caaaaacatt tgaaggtgca tattcaacaa
    15001 taaatgctaa gtttcaagct acaggtaatt cagacaaagt attattggaa ttgatagcta
    15061 aggtagaagg ggctactggt aaaaagccta ttatatatgg tactccactt gcattaagta
    15121 atttagaagg tattcaaact gatttagaaa aagaagaaaa aagaaaatat ggttttatac
    15181 aaacatttag aggtggatat aaagtactta gtttaccaaa tgcttatgat gaaaatgcag
    15241 cagaaggtaa agagtgggct ttagataata aggctatcta tgtaatacct gatggagaga
    15301 agataatcaa acttggtact gaaggagatg tattggtcat agaaaatact gatgaaaaag
    15361 aaagagatga ccaacaaata gaatatttta tggctcaaaa aatacattta ggagttgtaa
    15421 ctgctgctaa atttggagtt tataaaatac aataattgaa ttagaaggtg gataaccacc
    15481 ttcttttttt atgggaggga gattattatg gcaagagcta aaaaagaaaa tatttctaaa
    15541 acagaagtca aagctaatac tttagaaaaa gatataaagt ctactttaaa taaaaaagaa
    15601 gctagattaa accgattaga actacaaaga aagctaaaac aaaaaaaaca ggaaatagat
    15661 atagaaataa aaaatatatc cacatgggat gttgattata ttgacccaag agaaaaacaa
    15721 caaatttttt ctttatctaa agtaggttca aaagattcaa ctgaatttat agatttagat
    15781 actctttata gaatagtgag aagaagtcca ggtttttttg aagaacatag attaataatt
    15841 tcggatgtag attctttaga tgttgaatgt accccaatgg acataataga ttttctagga
    15901 ttgaatagtt tatatgaaca tataagaaat cctaatgaag actatttaga atatttctta
    15961 tctgataaag ttgatataaa ttcttttgaa aaaatattaa ataaaaataa tgttgagtta
    16021 acaagaagat tagctgagag ggcaatagat ttacataaaa agaaaaagtt tgattctggg
    16081 ctaaaggcta aactattagc taaaagaatt ggaatagaag atttgtactt gtttagttaa
    16141 aggtggtgga atatttgggt actccacttg taaaaatata taaaaagttt ttggatgcta
    16201 ttaatgatga agagatgtta ttactatcaa atgaaataat agaaaaaatg atgtacagtt
    16261 atttagaaga tgctatagtt gattttaatc aatgtaaaaa agatttgact ataaaatatg
    16321 ttgatgaaaa aggcgaggaa attattcctg ctgctcaact aagttataca tctaattata
    16381 gtaataaaaa tgcagagata actttgatgg gaaaagagac aaaagaagaa tacgaattag
    16441 ataaagatta tacaataagc tttgaagatg aaaaatttat aataagtttt gttgtagaaa
    16501 ctacagaaga gataattttc aaatacaaat atttaggaga aatagtatct gatttagatt
    16561 tagaggaaat taaaatatta tcatatggta tgcaaataca ttggttacaa cctaagataa
    16621 atagagaaga aaatttaaaa cagatgctaa ctgattctga ttataatgct aaatcaggtg
    16681 ctaatatgtt ggctaaactt caagcaagag aagaacaatt gagaactaga tttaataaat
    16741 atcaacaaag atacatgcta aaaaattttg aaggatggaa ctagcatgag ttacttaagt
    16801 gatataaaaa aaagaattgg acttggttgt tcaacaccta aagaaaaaag aatattacaa
    16861 ctaagattaa gttttaagaa atatttaaaa gaaacaccaa cctgtattga agttcctata
    16921 accgatatag atgaagtttg tataaatgaa gatactaaaa aagctgtagt tgcaattaat
    16981 gatataacta ataatgataa aagagcttta gatgaaaaaa atctattagt tgaatctgat
    17041 ttagatgtag atgtgggttg ttatcttttc tatgataatt gttattggtt aactatattt
    17101 aaggaacata aagaaatgga tacatataaa catttcataa taaaaagatg caatcaattc
    17161 tttaattata aatataaagg tcagatgtat aaaatcccta tagctgttga aaatttaacc
    17221 ttatattctg atggtatggc agataacaaa tatacttcta tctcagatac aaaaagacaa
    17281 ctttattttg gaagtaatcc tgtcactaaa actatagata ttgacactag aataatgtta
    17341 actggtaaaa ctgtatttag agttactagt attaatgact ttgagtataa tggcagagaa
    17401 acgggtgcag atggtcttat taaagctatt tgtttacaag atgcattgat ttcaaaagat
    17461 gatacaataa ataatattgc ttggaatgat ttatctgaaa atgatagtgt gattattcct
    17521 ttcagtaaga ttataggtta tgaagtaata aatctaggag aagaaaatga gtacataata
    17581 gaccattttc aaggagttaa atggctctta gacaagcaat acggatattg tgatataatt
    17641 tatcaagatg agaaaagatg tgtcatacag gctaatacat tggctaagta ttctggctta
    17701 gaagccttgc tattggctaa agataaagat acaaatgaaa taatagatac taaaaaaata
    17761 acactaaggg ggtagatata tgggtctata tggctttccc taacaagatg ataagtaata
    17821 taggaacaac tctaatgtca aatcaagact tcgctaaatt tatgatttat aataatgaat
    17881 cacaaaaaga tattttatcc atgcctgata taaaaaatcc agttaaagag cttagaaaca
    17941 agaaagtgtt ttttaataga agagtagaaa aagtattaaa agaagcagat gtttctgttt
    18001 acataacatt atttaaatat aatctttata aagatagaag tagaataata aaaactacta
    18061 atattgaaat tggtgtagta tgccatgatg aatgccaaga tacttttaat ggactaagag
    18121 atgctgcatt agtatgttgt atagtggaca caatcactga aaatgaggaa atagcaggta
    18181 taggaaaaat aaaattagag agctgttccc ccatgtttaa tttagataca agttataatg
    18241 gatttagcat agttgtatca tctgaaagtt ttggtgatat gtaatgttgg aaaattatta
    18301 tattacagga ttaccaatta agctaagtga aatattagga actatatatc aacctactat
    18361 tgaagaactt attaaatttg atatggctaa tttagagata gttaatccat ttttagtact
    18421 tgaaaagagc tattctcagc tatgtaacga gaaaacattt gaattgaaat gtaaatatga
    18481 tgctatacct atcttggatt tgatgatgtt aacatcaaga aaagattctt ctgaagaaat
    18541 tgagttactt agtgataaga taaaaaaatc tttatctata ttatataaaa ctgatattaa
    18601 aaatattgaa tatatgaata agattaaaat tggtatttta attaaatttg atgataaaaa
    18661 gaaaaatgct tttataagta gagaggattt tggactagta tcagatttga ttttagagat
    18721 gttttgtatt gataaaaaga atttatttaa agatgatgaa gataagtgga ttgaaaatac
    18781 tggctctgaa agagaaaaac agttgattgc acactttaaa gagaaggaaa gaaagaaaag
    18841 agaaaaagaa gcatatcact tatgtgatta tataaatgta gttcaaaaca tagatggata
    18901 tgtaccaata gatgttattt taaaaatgac ttattggcaa ttaattaatg tttataagac
    18961 taagatacaa tttaaaaact ataatgaaag tttaggctat gcatggtcat ttaaatatca
    19021 aacagatatg gataaaatga agcattggtc taaagagata aaaataccac ttagcactgt
    19081 taaatagcag tgctattttt tatgcaaaaa atgagaggat gattttataa tatgaaaaga
    19141 tttgcagtga aagatgcagg taatgtaata gtaaaaaata aagtaacagg agaagtattg
    19201 ttttattcac aggatttaaa tgcttttaat tttaagatgg attcagaatc agtttatgca
    19261 aaagctaaag gtgcaaatac aatagctttt gatggagcaa taacagcaag tttaacaatg
    19321 gaacaagaag taatacaaat ggctcaatta gcgatgttat tatcttctga tatagatgaa
    19381 aaaactgcaa aggttgggaa aagaaaagta ttaacatctg atagtactaa gaaagttact
    19441 ttagaaaata taaagccagt tgcaaatagc atatctgtat atagtataga aagtgatgga
    19501 atatctataa ttaaaaaatt acaattcact tcatcagtta caggagctaa tacagaaata
    19561 acaatatcta cagccgattt taatgcggga gataaagtag cagtattcta tttagaagaa
    19621 ataccaaaag caaaagttgt aaaaataaaa gaagaatcta ctgcaccaaa ttacgttgta
    19681 gaagctgagg taatggttaa aactgtggat ggtgaatata tggttttata catgagtgtt
    19741 ccaaatgcaa aagcacaaag aagtatagag ttaaatctta ctgctgagaa tccatctgga
    19801 tttaatatga cattagatgt tttaccagat gaaaataaag agtatgctac attcacattt
    19861 ataggagatg aaaatcttaa tcctgctaga atggcttcaa tgctaggtgt tgaattagaa
    19921 gatgaaaaag atactaaacc taagaaatag taaaatgccc tactcttaat tgagtaggga
    19981 ttattttttt acgtttaaat gtggttttaa tcggatttga acttaaaaaa atgaaagaag
    20041 gtgataaatt ggtattttta gatgataagt ttttatttga taatgtttca agtgaatcta
    20101 tgaacatcaa attagttaca ttaggtgatg atgaaatact gaatgaatat ggattgcctt
    20161 atgaagaagc aattaaatct gatagcagtt ttaataaaaa tccttgttac agtgaagatg
    20221 aaactactat agaacctata acattacaat tctgtttatg tagcgaacat ggggaggctt
    20281 atgaatggga tgattatacg ctagaaaaca tatgcaattg gttttggcag agagaattta
    20341 aaccttttat cagttatgac aatatagaag aaatatacta tttcaaagcg agaaaaataa
    20401 taaaaagata tacaaaagat aaaaaaggcg tattagaaat agagtttcaa ccatatacaa
    20461 attatgctta tagaagcttt caaaaagtta taactgtcaa ggatacaagg gaaattaaac
    20521 taaataatgt gtctaatgta gatgaagaat atgctccagt aattgatatt gaatgtctaa
    20581 aagaaggaga tataactatt agaaattcta ttatcagcga taatgaagaa gacaatttag
    20641 ttataagtgg attagaatta aatgaaaaaa taacaataga taatttatac tatactgttt
    20701 taaatagtaa tggagagaat aggtttaata ttattaatag aaagtggatt agattaagaa
    20761 gaggtgtaaa tatattgaag tttactggaa actgtaaagt ttctattaag tgtaaatatc
    20821 caataataaa ataagggaga ggtaaacatg aataaaatac aagtagataa attaatacaa
    20881 gatgaagtta gggctataat accaattata gatgagaatg gtaaagagga atatatagaa
    20941 gttagaaatc ctgataagga gactaaagaa gagatattaa ataaaatatg ggttggtatg
    21001 gagaatcctg atttagcatt gtctcaagaa gatattctta aaatgttgat tgataaattg
    21061 actaatatag agttaaatat tgaaatagaa gatttagtag atagcaatat atctagcgaa
    21121 ttagaaactg tgatgtatca catatgtcaa atagaaaatg aattaactgc atctttatta
    21181 atgaacactg atgttaaatt aggtcagatg aagaatgaga tattacaaga cagggtttta
    21241 aaagaaactg aagaaattga aaaaatgaat aatattaaag acaaggtggt gaattagatg
    21301 gtatttaaat cattggatga gctagttgct tatacaaaaa ataagattgc tgcatctatg
    21361 ccagaagttg gaaaagaaat gaaagaaatt atgaaggaag aagtaaataa acaggtttat
    21421 tctggttata ctccagttgg agaatggaat cctaatgttt ggggaggtag aacaggacaa
    21481 ttattagatg ctatagatgt tacagaactc tcttctaatt ctgtcacttc agagatacaa
    21541 aataatggaa attgggtgga tgcatttaca cgcaattcag catttccaat ggaaagatta
    21601 gaaaagggga aagtctgggg tgttggcgga tatagacctc aaacaaatat aatggaagag
    21661 agtaattcta aagcacaaga gagagtacct aagtgtttta agtctaaaat gaatgaatta
    21721 ggcgtaccag taaaatagat aatatcgagt gtctattaag ttatagagtt ttctaagggt
    21781 tgtatgttat ccactagtag atgtatatca taagtggaaa attaaaaagc ggtgatgctc
    21841 taacatatag tgagcaattt agcctagata ggtagtttag ttgatttgct tagataagcg
    21901 gtttagaatt gtttctcaat gttctagtac attggttgat gcaaaaatac atacaaggat
    21961 attgatataa agagagaggt ataattgttt caattaacat ttaaatttga atatatcgag
    22021 gaactattag aagttacaag aaaattagat tatcctaata tacattttag ggaatcttgt
    22081 agtaagataa tagaaaagta agaattaaaa aaagatagtt ttacaaataa taaagtcaat
    22141 attccaaaac atttaatata taattatgat gatgatattt ggactggaga tataataaaa
    22201 atggctaatc aggcaaagaa aaatgattat agcttcttta tgttcaatga tataatttat
    22261 tttgtagatg atgaatgtat aagttataag ataggtttta tagattaaat tgtaaaattt
    22321 ataagacgtt tattattata ttaatataga taaagcgagt gaccatagta cacctttaag
    22381 gtgtggagat agaataaacc ttttattatt ataagataat agaaggttta ttctattgta
    22441 atataaatat caagatgtat taataaatat taaagataat caatttagat tgttttttaa
    22501 attgaatcat ttatgttgta gttatttcaa tgatgacatg tgtgtgacaa gtataataca
    22561 cttggaaata ttgaaagata tataatacct taattgctta gaatcgaaca taagaggttg
    22621 atttttatgg ttgaaagtat tggaatttca acaagtgttc ctgagaggaa cattttgttt
    22681 ggtgtttagc tactataaga gcttatttta atgtttgagt gtgatgggat tgaggagcta
    22741 ttacttgaga gtagatgatt tactggaatg gtttatcatg tgaattaaat tccgtagctt
    22801 ctaactagcc ttaagtttaa gggttaccta tttataaaaa atgacagata atgagattta
    22861 atatgtagtc aaagatgtag tagaaaaaat aggatataaa gatgtatctg acaccttgaa
    22921 aaacatattg atgaaaaagt taaaaatatt aaggcagctt atatagctgt cttttttgta
    22981 tagaataaaa aagtttatta taacagatag ttattatttt attagtgtgt taccaagaat
    23041 aaattccttt tatctaatat ttattacaaa taatatatat atttatctaa tgaatgattt
    23101 tatattttag tgtaaattgg tatattttac catttacact tgatataata aaaaatataa
    23161 actatttaaa tagaattagg ggaattgtat ggaaaagtat ctttttaatc aaaaactatt
    23221 aaaagaaaaa tataatatgg aaatagacct agacttatat gactataaac aaagacgatt
    23281 aattttagaa aaatggagag atgtaattga taataaatct attgataaat taaatgaaat
    23341 tcaacttaag gaaatatttg tatcggatat tttaaacaaa gttcttaggt ataaaagtct
    23401 tacggacaat ataatggatt ataatataaa accagaagaa aaaactaaga ttgatggaac
    23461 aagagctgat attgtactag gctatttttc taatgaagaa agtgactata gagtagcaat
    23521 agaattaaaa aaaccaacaa ctaatttaga tgcaaaacaa ctaagaaaaa acaatcataa
    23581 tacaccagta gaacaagggt tttcttactt gcctaaatat ggaagaaatt gcacatggct
    23641 tatcgtctct aacttcaagg aaattagatt gtataatgct aatgatgcta cagagtatga
    23701 atttttttct attaaagata tatatgaaaa agatgatgta tttaaaaaat ttttatatct
    23761 tttgtcatct aaatctttaa tgaccaaaca tggaaaatct agggttgaag tactttggga
    23821 agagaatata aagcaagagg aagccataga aaaaggtttt tatgacctat ataaaaatac
    23881 tagattaaat ttatttaatg atatactttt aaataataaa gatattttgg atgtaaaaac
    23941 ttgtttaaca aagacacaaa aaatattaga tagagttata tttgtttgtt ttgcagaaca
    24001 taaaggatta ataccagaga atatctttga aacatgtatt caagtagggg aaaaaagtgt
    24061 atctaggttt ggagtctggg aacaagtaaa gggtctgttt gaatctatag atttaggtaa
    24121 tcaggaaaag ggcataaatc aatttaatgg aggtttattt gcagaagatt atattttgaa
    24181 taatctaaag ataccaaata aatgtttcga gaatttatac aagttatctt tatatgattt
    24241 taacagtgat ttaaatgaaa atatattagg gcatatattt gaaaaatcta tatctgactt
    24301 agaagaacta aaactagaaa taaatcaagt taatattaag aaaaaagata gtaagagaaa
    24361 caaagatggg atattttata ctcccaaata cataacgaaa catattgtta ataacaccat
    24421 aagaagatat ttagatgaga agaaaaaaga attaggcgaa gataaacttc cagaactaaa
    24481 agaagaagat tatgaaacag ttgtggttgg taggaatata cagcataagc gtgtacaagt
    24541 tgtagaatat aaaactgata aaattcaaaa atatataaac ttttggaaaa attatagaga
    24601 cgttgtaaaa aatataaaaa ttttagaccc agcttgtgga agtggagctt ttttgaatga
    24661 ggctttttct atgttaaaaa aagaagctga atcagtaaac gaaatacttc gtgatttaac
    24721 cgatggtgag atatcatttt ttgacttaga tgctaatata ttaaaaaata atttatatgg
    24781 agttgatttg aatgaagaat ctgtggaaat aactaaattg tctttatggc ttaaaacagc
    24841 taacaagtat agtttattaa ctagcttaga taataatata aaatgtggaa attctattat
    24901 tgataacaaa gaggttgatt gtgctaaagc ttttgattgg aatgttgaat ttgaagatgt
    24961 aatgatgtca ggagggtttg atattattat tggaaatcct ccatatgttt caactaaaat
    25021 gattccagaa aagcacagag aatattattg gaaaaaatat aaagatttac taattagtga
    25081 aatggattta tatgaattat ttttatatga gttttgcgaa aataaattaa agcaagatgg
    25141 atttttggga tttataacac ctaatacgta tttcacaaat aaaagctttg aaaatttaag
    25201 aaaatattta cttaaaaatg tatgtgttga tactatttta gactttccat atagattttt
    25261 tccttttgag gatgtaaaca aggaaaccgc tataattata attaataaaa aattgccgaa
    25321 tattaaaaat aatataaatc ttataagtat aaataaagaa aatatgaaaa agattaagat
    25381 ttttaatagt aatacataca actctgaaaa taaaattact ataaaaagta ttataaatga
    25441 tttggataat aaaattgtga tacaaagcaa cccaataata ttaaaaatgc tcaaatgtaa
    25501 gcataaatta ggggattatt taaatttaca taaaggttgg atgagtgtcc ctaataaaac
    25561 aatttgtgat ggaattatat atgataagaa aatattaagt agtaatgata taaaagataa
    25621 taaccaatta aatggaattt taaataaatg tttggaaggt aaggatatac atagatacta
    25681 tattgataaa gtagataaat ttgtaaatat tgaaaacatg gatgacaaaa ctatgaggtg
    25741 gcacaaatca cctaagataa taacacaaag aatagttggt caaaatcaaa ataagataat
    25801 tgcaacagta gatattgaag gtactataat attccctagt ggaaatatca taaatttaat
    25861 taaagaagat aatttggaag atatatattt ttatatagga gttataaatt ctagtttaat
    25921 aaattacttt tataataaat tttatggaga atcaaatacc aacttgacag cagatgcagt
    25981 aaaaaatata ccaataccaa a
//
LOCUS       DAMJYR010000046        25620 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.046, whole genome shotgun sequence.
ACCESSION   DAMJYR010000046 DAMJYR010000000
VERSION     DAMJYR010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 25620)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25620)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25620
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.046"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(<1..985)
                     /locus_tag="PC471_001876"
     CDS             complement(<1..985)
                     /locus_tag="PC471_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HDF2789006.1"
                     /translation="MKRVYIDFEMNMPNSKSKRAVFNSDIIAIGAVVYDEKTKNIDKF
                     KSLIRPVSNEELYPHIQELTHISSEELKSAPSYEEVMRKFKKWLGIFSDIKGIYTFGN
                     LDLTCFNNTDMRSAKKNNHPRFVNNIRDLFVDIKEKYINCGMRCMNYISLKNLLEFVN
                     LEFSGDAHDPLNDAYNLFILDEAITNNVDIQNLLIIRDIIRPPFNDINSNLDNCFNKF
                     KESLYKKEGNYNIVDFSVEIIKTVRMYLLTIIDVNIQNLEIMKDISKKMDTIDKLKDI
                     EEGYFYLLEDVYFDMKDVLEDLMLYRMNEDEYKYEIKNIIKMFDEDLDNEKI"
     gene            complement(1033..1893)
                     /locus_tag="PC471_001877"
     CDS             complement(1033..1893)
                     /locus_tag="PC471_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011986089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HDF2789007.1"
                     /translation="MSELSGKGCEIIVPFEERLPVRDIEKSIIKKYRKNLWSKFMKAI
                     RDYKLVEEGDKIAVAISGGKDSILMAKMFQELKKHGQVNFDVEFIAMDPGYHANIRQL
                     LIDNCEYLNIPIHLFDSRIFEIADEIAKDYPCYMCARMRRGALYSKAEELGCNKLALG
                     HHYDDVIETTMLNLLCAGNFKTMLPKLNSTNFEGIKIIRPLYYIREEHIIRFIQNSGI
                     WPLNCACMVAAKKTGNKRYEIKDLIKSLESNFKNVEKSIFKAAENVNLDSVLGWQKDG
                     EKHSFLENFE"
     gene            2162..2371
                     /locus_tag="PC471_001878"
     CDS             2162..2371
                     /locus_tag="PC471_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat associated protein CotJA"
                     /protein_id="HDF2789008.1"
                     /translation="MENKTRDIKKNCGPSLARPYINNQIFTEMYCLSDALKFGTIFPE
                     LNLLESEMYNKELYAKPKKYRGGRR"
     gene            2371..2637
                     /locus_tag="PC471_001879"
     CDS             2371..2637
                     /locus_tag="PC471_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat protein CotJB"
                     /protein_id="HDF2789009.1"
                     /translation="MKDSSRAELMRKVQETSFACVDMNLYLDNHPDDKNAINTYNSLC
                     NQFAQARYAYENKYGPLTNFGYAPSRCPWQWADQPWPWDREFNY"
     gene            2666..3238
                     /locus_tag="PC471_001880"
     CDS             2666..3238
                     /locus_tag="PC471_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000265278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HDF2789010.1"
                     /translation="MWIYQKTIQHPVNIKTCDPRMAKFLITQFGGPNGELAASLRYLS
                     QRYTMPTGNMRALLTDIGTEELAHVELICTMVYQLTSDASPEELKAAGLGSNYAQNGY
                     GIYPTDSNGVPFDVRPIAVMSNPVTDLHEDMAAEQKALATYYQLINLTDDVDVIDVLK
                     FLGQREIIHYQRFGEALMDAYELEESQKMF"
     gene            complement(3473..4663)
                     /locus_tag="PC471_001881"
     CDS             complement(3473..4663)
                     /locus_tag="PC471_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HDF2789011.1"
                     /translation="MNFNQKRIAASIMATAIIMPTMGNLAYANESEVESVSIESRTIT
                     GNAVNFRKGPGTNHESMGKLYKGDKVEYVGKEGSWVKVKYNGNTGYVHGNYVAINSLG
                     SSNESSDTSVKSTKVVTAKGLNFRTGPSTSSSKISTLGYGTEVGYISESNGWSKISSN
                     GRVGYVSSKYLGTSVNDSTNENAENSSNDLVKGTKVVTAKSLNVRTGPGTSHSKIATL
                     SYGTEVGSISESGGWTKVSYGNQTGYVSSQYLAEKGSVDTSIPSYSTNSPSQGADSVI
                     SFAKTLLGKPYVWGAEGPNSFDCSGFTQYVMKKSVGVSIPRVSRDQSKYGTYVNRGDL
                     RSGDLVFFDTQGSNNGSVSHVGIYIGNGDMIHASSGSSKKVTISNINSSYYSSRYVNA
                     RRVL"
     gene            complement(4923..5354)
                     /gene="dut"
                     /locus_tag="PC471_001882"
     CDS             complement(4923..5354)
                     /gene="dut"
                     /locus_tag="PC471_001882"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HDF2789012.1"
                     /translation="MYNLKVKKLNDDAIIPNFAHKGDAGMDLYSIEEVVIPPGETKLI
                     KTGICIELPTMTEAQVRPRSGLALKHSVTVLNTPGTIDEGYRGELKIILINHGKNDFK
                     VEKHMKIAQMIVKPIYDINIEEVKELSDSERGKGGFGSTGF"
     gene            5467..7695
                     /locus_tag="PC471_001883"
     CDS             5467..7695
                     /locus_tag="PC471_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IA DNA topoisomerase"
                     /protein_id="HDF2789013.1"
                     /translation="MKLVLAEKPSVAKTIASFLGAKTRQDGYFEGNDYIITYAVGHLV
                     SLYDMKDYDKDKYSGSWKMNNFPFVPEDKFKFKIDSSKTKQFNTVKKLLNRKDVEYVI
                     NATDNDREGELIAFLIFLLAKNKKPVKRILVNEWTPEDITRGIKNLKDEDEMRNLQAA
                     GYTRLITDWLIGINFTSVATLKYGNGKLLNIGRVILPTVKLVYDRDMEILNFVPKTYY
                     EIEGHFKAEAGEYKGKYVKGKESKFDTLEDANKIIASITSETGKILDKKVTMSKEYAP
                     KLFSLTSLQGYITSKYSNFTSDKVLNVCQSLYEGNGKGGYITYPRTDSIFLEESLASK
                     ASQTLDKLKVGLEYENKIKFSKTKRVFDSSKVDSHSAIIPTYIIPKSLSKDEQLVYDA
                     IKDRFVANFMPPAEYENTEIKTEVDNCTFLTKGKVLKSKGYLEVYNKEEKNDLLPLVN
                     KDDVVDVLEIKPLTKQTTPPKPYTEDTLLKAMKNCGKNVPEEDTTVLSGYSIGTSATR
                     ADVLKKISQVGYVKKKGKSYSITDLGKNLVEIFPVKDLFDVDYTGKLEKSLSDIQKGK
                     YTRKEYLTNIISFIYQNVNIIKQDASKNINTEAYTFDSKTKKFTKTKEKKQKKDVTNT
                     EKEVAISKDSNNKKEVKNGDENQSLGKCPICQGDVLEFDKGFACKNHKECKFVIWKND
                     KYLSLYKKKVNKTMVKNILKKGETKVKSLTAKNGNKFDATLKYDKNPDTGYFNWKIEF
                     DN"
     gene            complement(7806..8291)
                     /locus_tag="PC471_001884"
     CDS             complement(7806..8291)
                     /locus_tag="PC471_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDF2789014.1"
                     /translation="MDNNNILKIADSFLDFLFVLENNIFKESDLLKKFQNNSDIMKAY
                     FEECPMAPSHAKVILYLMTSNSSSISQIASNLGILKSNMTPIIDRLVEHGLVNKFPDP
                     KDRRILRVELTDKAFKLFDAVKAILKESLVKKLSNLSEEELTLLDEHTLKLSEIVKKL
                     G"
     gene            8543..9766
                     /locus_tag="PC471_001885"
     CDS             8543..9766
                     /locus_tag="PC471_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HDF2789015.1"
                     /translation="MKRYLIVFLMCSSLFLVGCGKTEESEPEKPIAVSVQKAIGGEIE
                     NTNSFSGTTKVKDETAVTAQTVGTVQEVYVKLGQNVRKGDELLSISSPELENSVKQSK
                     ASLDLAKASYSSATGGSLEAQVNQAKTALDNAKIQYEESQRNYDNNKILYEQEVISLD
                     QFKKIEFSLEQTKQQLDSAQRAYDTATSKSIPQAKALAKKQLDQAQVSYNLAMSNLDK
                     LTLTSPVDGTITAKNFDSKEMITQSQPAFIISNPSILEVDLNVAESDIGKFKKDGNVD
                     VIIEDQRILGKIDYVPSVVDPQTSLYPVKVLVNNTNNKFKAGMSAQVNLSVEKENGAV
                     TVPKKAIFEENGKKYVYIATKDNIAKKHLVQTGIVTEDKIEIKSGVSDKDTVIIGGIS
                     LISDGTKIFPVEKEK"
     gene            9770..12904
                     /locus_tag="PC471_001886"
     CDS             9770..12904
                     /locus_tag="PC471_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HDF2789016.1"
                     /translation="MNLTQTSVKRPLTIIMVFLVLIVFGGIGYKKMSINLMPDIEIPV
                     VMVMTTWTGAGPQDVDEQVSQKVDESLSAVSNVKSTISSSQESVSMVVAQFEFGTNLD
                     EIMNDVRSKVDALQTSLPDDAAKPTVLKLDMNAQAIGQLVISGGNENSSQALRKYAED
                     VIQPKIESIDGVTSADLKGGKKAQVNVIADPAVLSNYGVSLSTIKGVLSSSNKTFPYG
                     SITQGEDKIVLRAIDKLESLDDIKQIQIPVKGGNTVRLDNLCSVDYGYADKDSIYRYN
                     GKDSLVIDISKQQDANTVKVMQSAYKYVDELNKENPQFKIKIANDTSSYISESINSVM
                     SNLLISAVIAFIVIFAFLKSVRASLVVAVAIPISIIGAIAILYFTGESLNLITASSLV
                     ISVGMVVDNATVVIENIFKYRKSGRLSLDDCAIEGTRTVTNAILASTLTTIAIFLPIM
                     FTEGIAKITFGALGKTLIASLAFSFIGAITLVPSVFAKLSRGKNSQKMQEKDSPIFDK
                     VSEAYKKLLSVCLRHQRLVVISSLLIFVASLFGMGFIGMDFMPAADKGELSISIELPK
                     GLSLESNDYYVSMTEKKVSDIPEIKTLITTLTSDSNNSKASIAIELESQKNRKRTTDE
                     IEKEIIDRVATVPDCKINVSQNSSVMGGAGGADFTLEMKGPDLDVLKILGKEAEKEFS
                     SIKGFRNVETSLTDTTQEAQFTIDKHKAQEYGVNTAEIAGMLRTAINGDSVTTATIDD
                     YKVDVNLKFKADTIDSLEDIKQLKVNSATGKEVPLGAFSDIKMADGLQSISRTDGDFS
                     VNITATLDNLDAGTATKLALEKTNKMVIPKDYSIGVGGETEMMNESMASLGFSMVIAI
                     ALVYMVMVAQFESFSKPFIIMFCVPFAFVGVVLSLLVSGQSLSTVGMLGIILLIGIVV
                     NNGIVLIDYIEQLRKTNRNNDLIGLVSIGCAARLRPVLMTTATTILGMLPTALSLGEG
                     GETMQPLAIVIMGGLTVSTLITLVLIPTIYLIFDKWENKFSDKMPKIFGKITDKIGSI
                     KRKIIKKPKLKAKV"
     gene            12938..14236
                     /locus_tag="PC471_001887"
     CDS             12938..14236
                     /locus_tag="PC471_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HDF2789017.1"
                     /translation="MSKIVAIGCGVGIITANLIPVYADNNTSSINKVDNSILSDSNKR
                     TSEKEILTAREAVSAALKNSEKLKMKSEEIKMLKEKLEVQDEFDSFTGSDNSFPYDQI
                     GLLKNQSEQAKGFMEDQIANDITNKFNDLVSRENELDKIKNNLEIKTKEIKDMKLKKD
                     LGLVTSLETESAELELQTLQNTQKAKLQELKNNQDYFKLLTNIDLNNYQLDKEYRFES
                     FRVGGSVDSYMEGKVNEYLKYDQLILERTEESFNDKDENKADLPDRPNLTRPVAPTKP
                     EQGNLTDDEYKVLTDKYEKDYKRYEKEVEAYNLERQTYAAGLTTYANYLQQKFNTENG
                     LVTLEDSKKALKKGLIDSYAQLLALEDTIQITKKQLDLSEKQLKNTKLRYDLGLITLT
                     DYKKQVVSNEDAKNSYDTLIVNYNSLKNGIEKPWILNTGK"
     gene            complement(14525..14770)
                     /locus_tag="PC471_001888"
     CDS             complement(14525..14770)
                     /locus_tag="PC471_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HDF2789018.1"
                     /translation="MTKCPKCGKEVSSRPGSICPRCGFKVADESLKIRCPEPSCRALV
                     SRKLDYCPKCGCKLRGYNISEFVNMVRCKIDETFDKK"
     gene            complement(14984..17611)
                     /locus_tag="PC471_001889"
     CDS             complement(14984..17611)
                     /locus_tag="PC471_001889"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HDF2789019.1"
                     /translation="METKYVYSFGEGSKDMKSLLGGKGANLAEMTKIGLPVPPGFTIT
                     TEACNDYYVNNESIRAEIIKEIEEHLATLEKDLNKTLGCNKNPLLVSVRSGAVFSMPG
                     MMDTILNLGLNDNSVVGLAEATQNERFAYDSYRRFIQMFSDVAMEVPKYKFENVLDRV
                     KEAKGYTVDTELTTDDLKEIVKEFKAIYKKEIKNDFPQDPKEQLMLAIEAVFRSWNNP
                     RAIVYRKLNDIAHNLGTAVNIQSMVFGNMGETSGTGVAFTRNPATGENKLFGEFLMNA
                     QGEDVVAGIRTPQNISTLADVMPAVFDEFVKITHILEGHYKDMQDIEFTIENERLYIL
                     QTRNGKRTAAAAINVAVDLVEAGIIDEKEAIMRIEPNQLDQLLHPKFEDKALKEAKVI
                     AKGLPASPGAASGKVYFNADDVVKANEKGEKVVLVRLETSPEDIEGMVKAEGILTARG
                     GMTSHAAVVARGMGKCCVAGCGEIKVDEFNKEIRALDDVVIKEGEYISIDGSTGNVYL
                     GDVKKTEVSLTGNFEKLMNWVDKHKCMMVRTNADNPRDARAAIEFGAEGIGLCRTEHM
                     FFDEARLPAVREMILSNTVEQREKALEKILPMQREDFVELFKVMDGKPVNIRLLDPPL
                     HEFLPHDDETIEELSKSMGIKVSDIKKRIVDLDEFNPMLGHRGCRLAITYPEICVMQS
                     KAIIQGAIEAIKAGVKVSPEIMVPLVGEVNELKIIRKMIVETVDAIIKEEGIEVPYTV
                     GTMIEIPRACLTADEIAQEADFFSFGTNDLTQMAFGYSRDDAGKFLGQYVDEEILEKD
                     PFQVLDQNGVGKLVKMGAKLGREVKPELKLGICGEHGGEPSSVEFCYSVGLNYVSCSP
                     FRVPIARLAAAQASIKNPR"
     gene            complement(17672..18514)
                     /locus_tag="PC471_001890"
     CDS             complement(17672..18514)
                     /locus_tag="PC471_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HDF2789020.1"
                     /translation="MLVKNLIIYAVSDSVGETAQQVAKACMSQFYVNETYEIKRFPYM
                     INKGVLLETLENAKAENALIVYTLVDEELCSIVERYCEREGLSCIDLMTDILREISKR
                     TGRKPKREAGIIRKLDESYFKRVEAIEFAVKYDDGKDPRGVLQADIILVGISRTSKTP
                     LSMYLANKNIKVANVPLVPEIPIPKEVFEIDTKKIIGLTNSPEKLNEIRTQRLKALGL
                     SSKANYANLERILQELDYSEEIMKRIGCPVINVSNKAIEETAGIILDIMKENGLKIYK
                     EIEI"
     gene            complement(18521..19159)
                     /locus_tag="PC471_001891"
     CDS             complement(18521..19159)
                     /locus_tag="PC471_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2789021.1"
                     /translation="MIIIQLNERQLKIIDIVKENEPITSESIASSLNVTRATLRSDLA
                     ILTMTGILDARPKVGYFYSGVSEINLIGKSVKEKTVEDIMSMPVLAKKDESIYDVIVT
                     MFLSDVGSIVIIDENEELCGVVSRKDLLKATIGGSDINKMPIGMIMTRTPNVVTLTKG
                     ASVLLASRKIIEHEVDSIPIVEYKGEDKNHMRVVGRISKTNITKLFLEIVDN"
     gene            complement(19595..20887)
                     /locus_tag="PC471_001892"
     CDS             complement(19595..20887)
                     /locus_tag="PC471_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HDF2789022.1"
                     /translation="MLDLNRKLAKLEEEGKKIKVALIGAGHMGNGMVSQMANMKGIEA
                     SIVVDIDLELAHKAFTDAGIDEEIIDNVTNEKDAEIKLQEGKVLTCNDFLVACKTKSI
                     DVVIDATGGIAIGAEIALNSILNKKHIVMLNAETDCVVGPILKKLADDAGVIFTGSAG
                     DEPGAVMELFDFADAMGFEVRVIGKGKNNKLDLDCNPDTVREEAERKGASPHMIASFK
                     EGTKTMVEMALMCNATGFVPDVRGGHGIEATVNEVPKKYALKSEGGVLDNYGVVDFVN
                     GIAPGVFVVVAHKLKAVNDELKYLSMGDGPNYILYRPYHLCSLETPLSAAMAVLDHKA
                     TIVPKAGLVAEVMTIAKKDLKKGEFMDGYGAYTCYGTIEKYDVAKAMNAVPIGLISKK
                     TKVVKDIKKGEVITYDMVEIEKDTTLYHLRQLQEKIFG"
     gene            complement(20924..21280)
                     /locus_tag="PC471_001893"
     CDS             complement(20924..21280)
                     /locus_tag="PC471_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HDF2789023.1"
                     /translation="MKYEVKISGIGSLVKELMDESNCLIIYDETINDDDLKDISVIHS
                     IATLKSDVEIGDTLTIGNRDYCIVSVGDIAQKTLREIGHCTIKFDGKCEVNLPGEIHV
                     EIGNPDITIGDLITIS"
     gene            complement(21315..21674)
                     /locus_tag="PC471_001894"
     CDS             complement(21315..21674)
                     /locus_tag="PC471_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2789024.1"
                     /translation="MKLLVILFVAYILNTIFIMKQNKLYFKTLNRAKKKGDIVSTGKK
                     KSYFSKGSIAVISSDSDGFIKFGEILKGRTVMAKFKEIEEIEGLDIYSAEQKFKDEES
                     IVQAIKFIKEKINVSFN"
     gene            complement(21743..23716)
                     /locus_tag="PC471_001895"
     CDS             complement(21743..23716)
                     /locus_tag="PC471_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HDF2789025.1"
                     /translation="MSVTEKIPSIDRKIFKILIMCSKKEFVSINSIANELNVTTRSVR
                     TYIKQLNKDLGNDIAVIKYIKGQGYKLEIKDEQILNKIIDMNRKNVFSLNSKEDRVEF
                     ILNYLIELDGFITLDSLADEMCVGRTTLVNDFQYVEKVLASYNLNLIKKQNTGMKLNG
                     NELDIRLFILNQLYKNSRKDFSNSKYFKGIKKEEIINLEEKLLTLFKKNNFYVTDEML
                     REVINYIVVLVYRVKEEKKVKDYDVKFDLLKSYDEYFIAREIKHIISGMFECTLNDEE
                     IIYLTIPLVSGNAPASECALNSSRISKNIDELMENIFNQIYVDMGIFINEDELRVGLG
                     YHLSFTLNRLLFNIKLKNVLLEEIKQNYILPFKLAQIAGKVIEKKYNLEVSEDEIGYI
                     AIHFSGYLERNSSRFYSIKKIAIICSTGLGTAKLLKIRVEKLIGNNPKIDTLSSFNLR
                     NINLDEYDIVFTTIDLDTSNINTIVLKINTIFDENKLREQLKTVLCLREGNIDTTNST
                     NLLINNLLDEDKFMILNEKTILASLEKMMDHLMDLGCIDEKFRKNIVNREEKSPTVFD
                     KGLLFPHSINEKSDKFLMAVGILEEPIEYANRSIKIILMTMFPCENKIDSDLLVKIYE
                     EVLKIGQDVKLTNKISKCRTFLEFKKVLLKNLI"
     gene            complement(23938..24951)
                     /locus_tag="PC471_001896"
     CDS             complement(23938..24951)
                     /locus_tag="PC471_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HDF2789026.1"
                     /translation="MEENKILKIEKGTSGWGGPLYIKKEGNRNKILSMTAGGIHEVTL
                     KIKELLGCEIVDGFKTGVSDEEVAVVIIDCGGTARCGVYPKKKIPTINVNPVGKTGPL
                     AKFITEEYYVSDVNPNCISVVDGEDMPQKSQENKSENKSSIRKPDNYDEVKSKAQGEY
                     AKKNIILSIGQGAGQVVSKFYDAGRDTIQMVMNNVIPFMAFVSMLMGIILASGLGDWI
                     ARVISPLAGNIGGLLIISVICTLPFLSPILGPGAVIAQVVGTLVGTQIGLGAIPAYLA
                     LPALFAINGQAGCDFVPVGLSLGEAEPETVEYGVPALFYSRLITGPIAVIIAYGVAVF
                     ALR"
     gene            complement(24964..25509)
                     /locus_tag="PC471_001897"
     CDS             complement(24964..25509)
                     /locus_tag="PC471_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HDF2789027.1"
                     /translation="MENVITGLSKGAEWFIGLFQKGGEQFIGLVSGTLPTLIVLMVAI
                     NSLIKIIGEERVNNWASKLGKNSFTRYILLPLVSVFFLGNPMCYTFGRFLKEEHKAGF
                     YDAAVSFVHPITGLFPHANAGELFVWLGISAGLTTLGKETTTLALWYFIVGLIVIFIR
                     GIVTEKMYAFLTRKNRATNKA"
BASE COUNT         8762 a         4270 c         3266 g         9322 t
ORIGIN      
        1 atattttttc attatctaag tcttcatcaa acatttttat tatatttttt atctcatact
       61 tgtactcatc ttcattcatt ctataaagca ttaaatcttc tagtacatct ttcatgtcga
      121 agtacacatc ttctaataaa taaaaatagc cttcctctat atctttcaat ttatctatag
      181 tatccatttt tttacttata tctttcataa tttctaaatt ttgtatattt acatctatga
      241 ttgttaataa gtacattctc acagttttta ttatttcaac agaaaaatct acaatattat
      301 aatttccttc ttttttatat aatgactcct taaatttgtt aaaacaatta tctaaattag
      361 aatttatatc gttaaatggt ggtcttatta tgtcacgtat tattaaaaga ttttgtatat
      421 ctacattatt tgtgattgcc tcatccaaaa taaataaatt atatgcatca tttaatgggt
      481 catgggcatc tccactaaac tctaagttaa caaattcaag taaatttttc aatgatatat
      541 agttcataca tctcatacca caatttatat atttttcttt tatatctaca aataaatctc
      601 taatgttatt tacaaacctt ggatggttat tttttttagc acttctcata tctgtgttat
      661 tgaaacatgt caaatccaaa ttaccaaaag tatatattcc tttaatatca gaaaatatgc
      721 caagccattt tttaaacttt ctcatgacct cttcataact tggtgcactc ttcaattctt
      781 cagaagatat atgtgttaat tcttgtatat gaggatacaa ctcctcatta cttactggtc
      841 ttattaaaga tttaaattta tcaatatttt ttgttttttc atcatacaca actgctccaa
      901 ttgcaattat atcagaatta aatactgctc tttttgattt agaatttggc atattcattt
      961 caaaatctat atatactctt ttcaatataa acaccctttc caattgttta tagtgattct
     1021 ttttaactat ttttattcaa aattttctaa gaaagaatgt ttctccccat ctttttgcca
     1081 accaagtact gaatcaagat ttacattttc tgccgcctta aatatagatt tttctacatt
     1141 tttaaaattt gattctaaac tctttattaa gtcttttatc tcatatcttt tattacctgt
     1201 ttttttagca gcaaccatac atgcacaatt taatggccat ataccactat tttgaataaa
     1261 tcttataata tgttcttctc ttatataata aagtggtctt ataattttta taccttcaaa
     1321 attagtagaa ttaagtttag gaagcattgt tttaaaattt ccagcacaca ataagttaag
     1381 cattgttgtc tctattacat cgtcataatg atgacctaat gcaagtttgt tgcaccctag
     1441 ttcttctgcc tttgaatata atgctcctct cctcattctt gcacacatat agcatggata
     1501 atcttttgct atttcatctg ctatctcaaa tatccttgaa tcaaataaat gtattggtat
     1561 atttagatac tcacaattat ctataagtaa ttgtcttata tttgcatgat atccagggtc
     1621 catagcaata aattcaacat caaaattaac ttgtccatgt ttttttaact cttgaaacat
     1681 tttagccata agaatactgt cttttccacc agaaatcgct acagctatct tatcaccttc
     1741 ttcaaccaat ttataatctc ttattgcctt cataaactta gaccataaat tttttctata
     1801 tttttttatt atactttttt ctatatctct tactggaagt ctttcctcaa aaggaactat
     1861 aatttcacag ccttttcctg atagttcact catctaatca cctcgtatat ttatgttaat
     1921 aatattttac aaaaaagtct accttcatcc acaaaatact ataattttta ctttaatttt
     1981 actaaataat ttatagttta caataacaca taaacttata ataacataaa taacctcttt
     2041 cttttattat cttttttatt tttctcattt aatattttat tattagtaac gatttttaaa
     2101 tgattgaata ttatacacta gatagagtta cctatctatt tacaacaagg aggttttcat
     2161 cttggaaaat aagactagag atatcaaaaa aaattgtggt ccatctttgg ctagaccata
     2221 cattaataat caaattttca cagaaatgta ctgcctttct gatgcattaa aatttggaac
     2281 tatatttcca gaactaaact tactagagtc tgaaatgtac aacaaagaat tatatgctaa
     2341 gccaaaaaaa tatagaggag gtagaagata atgaaagact catctagagc tgaacttatg
     2401 agaaaagttc aggaaacatc ttttgcatgt gttgatatga acttatattt agacaatcac
     2461 cctgatgata aaaatgctat caacacttat aattcattat gtaatcaatt tgcgcaagct
     2521 agatatgctt acgaaaataa atacggtcct ttaactaact ttgggtatgc tccaagtaga
     2581 tgcccttggc aatgggctga ccaaccttgg ccttgggaca gagaatttaa ttattagaca
     2641 tcaagtattg gaggattgaa aattcatgtg gatatatcag aaaactatac aacacccagt
     2701 taatataaaa acttgtgacc ctagaatggc taaatttctt ataactcaat ttggtgggcc
     2761 aaatggagaa cttgctgcat ctttaagata tttaagccaa agatatacaa tgcctactgg
     2821 taatatgcgt gcacttttaa cagatattgg tacagaagaa ctagctcacg ttgagcttat
     2881 atgtactatg gtttatcagt taacttctga tgcaagccca gaagagttaa aagctgcagg
     2941 tcttggttca aactatgctc aaaatggata tggaatttat ccaacagatt caaatggtgt
     3001 tccatttgat gtaagaccta tagcagttat gtcaaatccc gtaaccgatt tacatgagga
     3061 tatggcagct gaacaaaaag cacttgcaac ttattatcaa cttataaacc taacagatga
     3121 cgttgatgtt atagatgtat taaaattctt gggtcaaaga gaaataattc actatcaaag
     3181 atttggtgaa gctttaatgg atgcttacga gttagaagaa tctcaaaaaa tgttctaaaa
     3241 tttttatttg tataactatt attatttgat aatttataat ttactgttgt gttaaaattt
     3301 ataaaaaata taaatataat ttattttcta taaacagaga aaataaataa aaagcaggta
     3361 tcaatcaaat agtaacatgt attatactat acattactat caatattgta cctgctttca
     3421 tattataagc aataaagaaa aatctattta ttaggtttta taataaaaac aattataaaa
     3481 ctcttcttgc atttacatat ctacttgaat agtagctact atttatatta gatattgtta
     3541 ctttttttga tgaacctgaa gatgcatgta tcatatctcc atttcctatg tatattccaa
     3601 catgacttac agaaccatta tttgaacctt gtgtatcaaa gaatactaaa tctccacttc
     3661 taaggtctcc tctatttaca tatgttccat acttactttg gtctctagat actcttggta
     3721 tactaactcc tacagatttt ttcataacgt actgtgtgaa tccagaacag tcaaaactat
     3781 ttggaccttc tgctccccat acatatggtt ttccaagtag tgtctttgca aaacttatta
     3841 cactatcagc tccttgactt ggagaattag tcgaataaga tggaattgaa gtatcaacag
     3901 aacctttttc agcaaggtat tgacttgaca catatccagt ttgattgcca taacttactt
     3961 ttgtccatcc tccactttca gaaatacttc caacctcagt accataactt aatgtagcta
     4021 ttttagaatg acttgtacct ggtcctgttc taacgttcaa tgactttgct gttacaacct
     4081 ttgtaccctt tacaaggtca tttgaactgt tttcagcatt ttcatttgta gagtcattta
     4141 cactagttcc taaatattta cttgaaacat aacctactct accattagaa cttatttttg
     4201 accatccatt tgactcagat atgtatccga cttcagtccc atatcctagt gttgatattt
     4261 tagaactact tgtacttggt cctgttctaa aatttaatcc ttttgctgtt acaacttttg
     4321 tcgacttaac tgaagtatca ctactttcat tgctacttcc aaggctattt attgctacat
     4381 aatttccatg cacataacct gtgtttccat tatatttaac ttttacccaa gaaccttctt
     4441 ttcctacgta ctcaacttta tcacctttgt aaagtttccc cattgattca tggtttgtac
     4501 ctggtccttt tctaaaattt accgcattcc ctgttattgt tctgctttct atactcacac
     4561 tctctacttc agactcatta gcatatgcta aattccccat agtaggcatt attatcgcag
     4621 ttgccattat actggctgct attctctttt gattaaaatt cataactttc cactcatctc
     4681 ctcttttcta ctatctttat ttcacttcaa aatataaaag aaataattga cactattaaa
     4741 atatcaatta tttcttttat attaatatta ttacaaattt gtaactattt gtctatagta
     4801 atatttgctt accataaatt accatgtata aattttaatc tttaatagcc tgtaatttag
     4861 taatatagcc atcttttacc ttttttaaaa atggctatat tttttaactt ttttgtaact
     4921 ttttaaaatc cagttgagcc aaacccacct tttcctcttt cggagtcact taattctttt
     4981 acttcttcta tgttaatatc atagataggt tttactatca tttgagcaat tttcatatgc
     5041 ttttcaacct taaaatcatt tttaccatga tttattaata taattttaag ctctcctctg
     5101 tatccttcgt caatagttcc tggtgtattt aatacagtaa cagaatgctt taaggcaagc
     5161 ccacttctag gtcttacctg cgcttctgtc atagtaggaa gctctataca tatcccagtt
     5221 ttgataagtt tagtctctcc aggaggtatt acaacctctt ctatagaata caaatccata
     5281 ccagcatcac ctttgtgagc aaaattaggt attatagcat catcatttaa tttttttact
     5341 tttaaattgt acatcttata tctcctttca actttctata aatattataa cttaaatttc
     5401 gacctcttga agttttttat ttttttgtgg taatataatt attagtgttt taaaggagga
     5461 ttttttatga aacttgtact tgctgagaaa ccatctgtgg caaaaactat agcatcattt
     5521 ttaggtgcaa agaccagaca agatggttac tttgaaggaa atgattatat aattacttat
     5581 gcagttggac acttagtatc attatatgat atgaaggatt atgataaaga taaatactct
     5641 ggctcttgga aaatgaacaa tttcccattt gttcctgagg ataagtttaa atttaagatt
     5701 gatagttcta aaactaaaca attcaacact gtaaaaaagc ttttgaatag aaaagatgta
     5761 gaatatgtaa taaatgccac tgacaatgac cgagaaggag aacttatagc atttttaata
     5821 tttttactgg caaaaaataa aaaaccagta aaaagaatct tggtaaatga atggacacca
     5881 gaagatatta caagaggtat aaaaaaccta aaagatgaag atgaaatgag aaatcttcaa
     5941 gctgctggat atactaggct tattactgac tggttgattg gaataaattt cacttctgtt
     6001 gctacattga aatatggaaa tgggaaacta ttaaacatag gtagggtaat tcttccaaca
     6061 gttaaattag tctatgatag agatatggaa atactaaact ttgttcctaa aacctattat
     6121 gaaatagaag ggcattttaa agctgaagct ggtgaatata aaggtaaata tgttaagggt
     6181 aaagaaagta aatttgatac acttgaagat gcaaataaaa ttatagcttc aattacatct
     6241 gaaactggta aaatactaga taaaaaagtt actatgtcaa aggaatatgc accaaaactt
     6301 tttagtttga catcacttca aggatatata acttctaagt attccaattt tacatctgat
     6361 aaggttttaa atgtctgtca atctctctat gagggtaatg gtaagggtgg atacataact
     6421 taccctagaa ctgattccat tttcttagaa gaaagtttgg cttcaaaagc ttctcaaact
     6481 ttagataaat taaaggttgg tcttgaatat gaaaataaaa ttaaattttc taaaactaaa
     6541 agagtttttg attcttctaa agtagactcc catagtgcta taattccaac ttatataatt
     6601 ccaaaaagtt tatctaagga tgaacaatta gtttatgatg ccataaaaga tagatttgta
     6661 gcaaacttta tgcctcctgc tgaatatgag aatacagaaa ttaagactga agtagataat
     6721 tgtactttct taacaaaagg taaggtgctt aaatcaaaag gataccttga ggtatataat
     6781 aaggaagaaa aaaacgattt acttccactt gtaaataaag atgatgttgt agatgtgtta
     6841 gaaataaaac cattaactaa gcaaactact ccacctaaac catatactga ggacacactt
     6901 ttaaaggcta tgaaaaattg tggtaaaaat gtgcctgaag aagatacaac agtgctatct
     6961 ggatattcga ttggaacatc agccacacgt gctgatgtgt taaagaagat aagtcaagtg
     7021 ggctatgtaa agaaaaaagg taaatcttat tctataacag accttgggaa aaatttagtt
     7081 gaaatttttc ctgttaaaga cttatttgat gtagattata ctggaaaact tgaaaaaagt
     7141 ttaagcgata ttcaaaaagg aaagtataca agaaaagaat atcttactaa tatcattagt
     7201 tttatatatc aaaatgtcaa tattataaaa caagatgctt caaaaaatat aaacacagaa
     7261 gcatatactt tcgattcaaa aactaaaaaa tttactaaaa caaaagaaaa aaaacagaaa
     7321 aaagatgtaa ctaatactga aaaagaagtt gctatatcaa aggattctaa taacaaaaaa
     7381 gaagttaaaa atggtgatga aaatcaatct cttggcaaat gccccatttg tcaaggtgat
     7441 gtactagaat ttgacaaagg ctttgcttgt aaaaatcata aagaatgtaa atttgttatt
     7501 tggaaaaatg ataagtattt aagtctttat aaaaagaaag ttaacaaaac aatggtcaaa
     7561 aatattttaa aaaaaggtga aactaaagta aaatctttaa ctgctaaaaa cggtaataaa
     7621 tttgatgcta cattaaaata tgataaaaat ccagatactg gctatttcaa ttggaaaata
     7681 gagtttgaca actaaatatt cataagaaaa gaaatagcag tagtataata atctatatta
     7741 tattactgct atttcttttc ttatgaagtt ttaacttttt taatagtgac ttttacaatc
     7801 acactctaac ctaatttctt tactatttct gataacttta atgtatgttc atctaataaa
     7861 gttagttcct cttcagaaag attagataat ttttttacta gagattcttt gagaatagct
     7921 tttactgcat caaataattt aaatgcttta tctgttaatt caaccctaag aatccttcta
     7981 tcttttgggt caggaaattt attgactaaa ccatgttcca ctaatctgtc aataatcgga
     8041 gtcatatttg attttaaaat tcctaaatta gaagctattt gagaaattga tgatgaattt
     8101 gatgtcataa gataaagaat taccttagca tgtgatggag ccattggaca ttcttcaaaa
     8161 taagctttca ttatatcaga gttattttga aactttttta acaaatcgct ttctttaaat
     8221 atattatttt caagcacaaa taaaaaatct aaaaaagagt ccgctatttt aagtatattg
     8281 ttgttatcca tatttgtaac atcccttcaa tagataatat aataaaacat tgttgtttaa
     8341 tatagtataa attataaact attaataaat ttaaaacaag aaaaagttat tgactcataa
     8401 taattataaa tttataatca aattagagaa ttataaattg ccaataaagg gaagtttttt
     8461 aattagtcta aaactaaact taaattataa gacactttaa attctacaac aagctagtgt
     8521 gcaatatata ataggaggag atatgaaaag atatttaata gttttcttaa tgtgtagttc
     8581 actgttttta gttggatgtg gaaaaacaga agaatctgaa cctgagaaac caatagcagt
     8641 ttctgttcaa aaagcaatag gtggagaaat tgaaaacaca aattctttct caggaactac
     8701 aaaggttaaa gatgaaacag ctgttactgc acagactgtt ggcacagttc aagaagtgta
     8761 tgtaaaactt gggcaaaatg taagaaaagg agatgaatta ttgagtataa gcagtccaga
     8821 gctagaaaat agtgtaaagc aaagtaaagc ctcactagat ttagcaaaag caagttattc
     8881 aagtgccact ggtggttcat tagaggcaca agtaaatcag gcaaaaacag ctcttgacaa
     8941 tgcaaagatt caatatgagg agtctcaaag aaattatgac aataataaga ttctttatga
     9001 acaggaagtc attagtttag accaatttaa aaaaatagaa ttttctttag agcaaacaaa
     9061 acaacagtta gattctgctc aaagagcata cgatacagca acttcaaaaa gtatacctca
     9121 agctaaagca cttgctaaaa aacagcttga ccaagctcaa gtatcatata atttagcaat
     9181 gagtaattta gacaaattaa cattaacatc tccagtagat ggtacaataa cagctaaaaa
     9241 ctttgattca aaggaaatga taacacaatc acaaccagct tttattattt ctaatcctag
     9301 tatactagaa gttgatttaa atgtagcaga atcagatata ggtaaattta agaaagatgg
     9361 aaatgtagat gttataattg aagaccaaag gattttagga aaaatagatt atgtaccttc
     9421 agtagttgac cctcagacat ctttatatcc tgttaaagta cttgtaaata atacaaacaa
     9481 caaatttaag gcaggtatgt ctgctcaagt aaacttaagt gttgaaaaag aaaatggtgc
     9541 tgttacagta cctaaaaaag caatatttga agaaaatggt aaaaaatatg tatacatagc
     9601 aacaaaagac aatatagcaa agaaacactt ggttcaaact ggaattgtaa cagaggataa
     9661 aatagaaatt aaaagtggtg taagtgataa agatacagtg attattggag ggataagtct
     9721 tatttctgac ggaactaaga tatttcctgt agaaaaggag aagtagacta tgaatttaac
     9781 acagacttcc gttaaacgtc ctctcacaat cataatggtc tttttagtat taatagtttt
     9841 tggtggtatt ggatataaaa aaatgtcaat aaatctaatg cctgatatag aaataccagt
     9901 tgtaatggtc atgactactt ggacaggtgc tggtcctcaa gacgttgatg aacaagttag
     9961 tcaaaaagtt gatgaaagtt tatctgccgt ttcaaatgta aaatctacta tatcaagttc
    10021 acaagaaagt gtttctatgg tagttgctca gtttgaattt ggaactaatt tggatgaaat
    10081 aatgaatgat gttaggtcta aagttgatgc attacagacc agcctcccag atgatgcagc
    10141 taaaccaaca gttttaaagc tggatatgaa tgcacaggcc ataggtcaac ttgtcatatc
    10201 tggaggaaat gaaaatagtt cacaagcctt aagaaaatac gctgaagatg taattcagcc
    10261 taaaatagaa tccatagatg gtgttacttc agcagacctt aaaggtggaa aaaaagcaca
    10321 ggttaatgta atagctgacc cagctgttct ttcaaattat ggggttagtt tatccactat
    10381 aaaaggtgtc ttatcctcta gtaataaaac attcccttat ggttcaatta ctcaaggtga
    10441 ggataaaata gttttaagag cgattgataa attagaatcc ctagatgata taaaacagat
    10501 tcaaattcct gtaaaaggag gaaatacagt tagattagat aatctttgta gtgtagatta
    10561 tggatatgca gacaaagata gtatttatag atacaatgga aaagacagtt tggtaataga
    10621 tatttctaaa caacaagatg ccaacactgt aaaagttatg cagagtgcat acaaatacgt
    10681 agatgaattg aataaggaaa atcctcaatt taaaattaaa attgctaatg atacaagttc
    10741 atacataagt gaatcaataa acagtgtaat gagcaacttg cttataagtg ctgttatcgc
    10801 atttattgta atatttgctt tcctaaaaag tgtaagagct tcactagttg ttgcagttgc
    10861 aataccaatt tctataatag gtgctatagc aattctctac tttactggtg aaagtttaaa
    10921 tcttattact gcaagttcat tggtaatttc agttggtatg gttgttgata atgctacagt
    10981 tgtaattgaa aatatattta aatataggaa aagtggcaga ttgagtcttg atgattgtgc
    11041 tattgaggga acaagaactg taacaaatgc aatacttgca tcaacattga ccacaatagc
    11101 aatattctta cctatcatgt ttacagaagg tatagctaaa ataacttttg gggcattagg
    11161 taaaacacta atagcttcac tagcattctc ctttataggt gcaattacac tcgttcctag
    11221 tgtatttgca aaattaagca gaggaaaaaa tagtcaaaag atgcaagaaa aagatagtcc
    11281 tatatttgat aaggtaagtg aagcatataa aaaattatta agtgtatgtt tgagacatca
    11341 acgcttagtt gtaatatcaa gtttacttat ttttgtagct tccttatttg gaatgggatt
    11401 tataggtatg gactttatgc ctgcagcaga caagggtgaa ttatctattt ctatagaact
    11461 tccaaaaggt cttagcctag aatccaatga ttactatgta tctatgactg agaaaaaagt
    11521 ctcagatatt ccagaaatta aaactttaat aacaactttg acatcagatt caaataatag
    11581 taaagcaagt atagcaattg aattagaatc tcaaaaaaat agaaaaagaa ctactgatga
    11641 aatagaaaaa gagataattg atagagtggc tactgtacca gattgtaaaa taaatgtaag
    11701 tcaaaacagt agtgttatgg gtggagctgg aggagcagac ttcacattag aaatgaaagg
    11761 tcctgattta gatgtattaa aaatactggg aaaagaagct gaaaaagaat ttagctctat
    11821 aaaaggattt agaaatgttg aaacttctct tacagataca acacaagaag ctcaatttac
    11881 tatagataaa cataaggctc aagaatatgg tgtaaatacc gctgaaattg caggcatgtt
    11941 acgtacagca ataaatggtg actccgtaac cactgctact attgatgatt acaaagtgga
    12001 tgtaaaccta aaatttaaag cagatactat tgatagctta gaagatataa agcaactaaa
    12061 agtaaactct gcaactggaa aagaagttcc tcttggtgct ttttcagata taaaaatggc
    12121 agatggtcta caatctatat ctaggactga tggagatttt tctgtaaata taactgctac
    12181 acttgacaat ttagatgctg gtactgctac aaaactagca ctagaaaaaa cgaataaaat
    12241 ggtaattcct aaagattact ctataggtgt tggtggtgaa acagaaatga tgaatgaatc
    12301 tatggcaagt cttggatttt caatggtcat agctatagca ttagtttata tggtaatggt
    12361 agctcagttt gaatcattta gtaaaccatt tataatcatg ttctgtgttc catttgcatt
    12421 cgttggagtt gtgctttcgc ttctagttag tggtcaaagt ttaagtactg tcggtatgct
    12481 aggtataatc ttacttatcg gtattgttgt taataatggt atagttctta ttgactacat
    12541 agagcaactt agaaaaacaa atcgtaacaa tgatttaatt ggtttggttt caattggttg
    12601 tgcagcaaga ttaagaccag tcttaatgac tacagcaaca acaattttag gtatgttgcc
    12661 aacagcacta tctcttggtg aaggtggaga aactatgcaa ccactagcca tagttataat
    12721 gggtggtcta actgtatcta cacttataac tttggtgctt atacctacta tatacttaat
    12781 atttgataaa tgggaaaata agttttcaga caaaatgcct aaaatatttg gtaagataac
    12841 agataaaata ggaagtataa aacgtaaaat aataaagaaa ccaaaattga aagctaaagt
    12901 ttaaatagaa tggagggtga ttgtattgaa taaaaaaatg agcaaaatag ttgcaattgg
    12961 ctgtggtgtt ggtataataa ctgccaattt aatcccagtt tacgcagata ataatacttc
    13021 ttctataaac aaagttgata attctatttt aagtgactca aataaaagaa catctgaaaa
    13081 ggaaattttg actgctagag aagctgttag tgctgcactt aaaaatagtg aaaaattaaa
    13141 aatgaaatca gaagaaataa aaatgttaaa agaaaagtta gaagtacaag atgagtttga
    13201 tagttttact ggaagtgaca actcgtttcc atacgaccaa attggacttc ttaaaaatca
    13261 aagtgaacag gctaaaggct ttatggagga ccaaatagca aatgatatta ctaataaatt
    13321 taacgattta gtttcaagag aaaatgagct agacaagata aaaaacaatc ttgaaataaa
    13381 aactaaagaa attaaagata tgaaattaaa aaaagattta gggttagtaa catctttaga
    13441 aactgaatca gctgaacttg aacttcaaac actacaaaat actcaaaaag ctaaacttca
    13501 agaactaaaa aacaatcaag actactttaa gttgttgact aatatagatt taaataatta
    13561 tcaattggat aaagaatata gatttgaatc ttttagagta ggtggctcag ttgattcata
    13621 tatggaaggt aaggttaatg aatatttaaa atacgaccaa ttgattttag aacgtacaga
    13681 agaaagcttt aatgataaag atgagaacaa agcagattta ccagatagac ctaatcttac
    13741 aagaccagtt gctcctacaa aaccagagca aggtaaccta actgatgatg aatataaggt
    13801 tcttacggat aagtatgaaa aagactacaa gagatatgaa aaagaagttg aagcatacaa
    13861 tcttgaaaga cagacttatg ctgctggcct tactacttat gcaaattatc ttcaacaaaa
    13921 atttaatacc gaaaatggtc tagtaacatt agaagacagt aaaaaagctc ttaaaaaagg
    13981 gttaattgat agctatgctc aattacttgc tttagaagat acaattcaga ttactaaaaa
    14041 acaacttgat ttatcagaaa aacaattaaa aaatactaaa ttaagatatg atttaggatt
    14101 aattactcta actgattata aaaaacaagt ggtaagtaat gaagatgcta aaaatagcta
    14161 tgacactctt attgtgaatt ataactcact aaaaaatggt atagaaaaac cttggatact
    14221 taatactgga aaataattct ggtaaataga aagattactt taacatacgt ttgattaaaa
    14281 aatagttatg cacactcgtg gctttagtca tggaagattc agaaggcaaa taatagaaat
    14341 agattagtaa aaatatattt gcttataaaa ctgtataatt ttatagaggc tatctcaaaa
    14401 tagagataag cctctttttt attgtttaaa ttataaaaat atatttaatt ccatacaaaa
    14461 agagtgcctt atgaactaaa aagttcattt tgagacaccc tcaaaagctt aactctaaat
    14521 ttaattattt tttatcaaat gtctcatcta ttttacatct caccatgtta acaaattcag
    14581 aaatattata tcctctaagt ttgcatccac atttaggaca ataatccaat ttcctagata
    14641 ccaaagccct acaactaggt tctggacatc ttatttttag actctcatct gctactttaa
    14701 atccacatcg tgggcatata ctacctggac gtgatgatac ttcctttcca catttaggac
    14761 actttgtcaa ctttatcccc tccagtaaac tcaatttaat tgatagttga aatttctctc
    14821 tacaataata tatgcaatat aattaaatac gtttcactta caactatata agttaaattt
    14881 ttataataaa aatcccctat attcaattta caaatactta atttgataca ttgaacaata
    14941 ggggatttta tttttattta gttacatatt ttgcagtcta ttattatcta ggatttttta
    15001 tagatgcttg agctgctgca agtctagcaa ttggcactct aaatggtgaa caagatacat
    15061 agttaagtcc tacactataa cagaactcta ctgaagatgg ttctcctcca tgctctccac
    15121 atattcctag ttttaattct ggtttaactt ctctacctaa tttagctccc atcttaacta
    15181 acttaccaac accattttgg tctagtactt ggaatgggtc tttttctaat atttcttcat
    15241 ctacatattg acctaagaat ttaccagcat catctcttga atatccaaat gccatttgag
    15301 ttaagtcatt agtaccaaaa ctgaagaagt ctgcttcttg tgctatttca tcagcagtta
    15361 aacaagctct aggtatttct atcatagtac ctacagtata aggaacttct ataccttctt
    15421 cttttattat agcgtcaaca gtttctacta tcatttttct tattattttt aactcattta
    15481 cttctccaac taatggaacc attatctctg gagaaacttt aacacctgct ttgattgctt
    15541 ctatagcacc ttgtattata gctttagatt gcattacaca tatttcagga taagttattg
    15601 caagtctaca acctctgtgt ccaagcattg ggttaaactc atctaaatca actattctct
    15661 tcttaatatc acttactttt attcccatag attttgaaag ttcttctata gtctcatcat
    15721 catgaggcaa aaattcatgt agtggtgggt ctagtaatct tatattaact ggttttccat
    15781 ccataacttt aaataactct acaaaatctt ctctttgcat tggtaatatt ttttcaagag
    15841 ctttttctct ttgttctact gtattagata atatcatttc tctaactgct ggcaatctag
    15901 cttcatcaaa gaacatatgc tcagttctac atagacctat accttctgca ccaaactcta
    15961 tagctgcacg agcatctcta ggattatctg cattagttct aaccatcata catttgtgtt
    16021 tgtctaccca gttcattaat ttttcaaaat ttccagttaa agatacttct gttttcttaa
    16081 catctcctag gtatacattt cctgtagaac catctataga aatgtattct ccttctttta
    16141 taactacatc atcaagtgct cttatttctt tattaaactc atcaactttt atttctccac
    16201 atccagcaac acaacattta cccatacctc tagcaacaac tgctgcatgt gatgtcattc
    16261 cacctctagc tgtaaggatt ccttctgctt taaccattcc ttctatatct tctggagatg
    16321 tctctagtct aactaaaact actttttctc ctttttcatt tgcttttact acatcatcag
    16381 cattgaaata aactttacca cttgctgcac ctggtgatgc tggtaaacct tttgctataa
    16441 ccttagcttc ttttaatgcc ttatcttcaa attttggatg taataattgg tctaattgat
    16501 taggctcaat tctcatgata gcttcttttt catcaattat tccagcttca actaaatcaa
    16561 ccgctacatt tattgctgct gctgctgttc tcttaccatt tctagtttgt agtatatata
    16621 atctttcatt ttctatagta aactctatat cttgcatatc tttataatga ccttcaagta
    16681 tatgagtaat ttttacaaat tcatcgaata cagctggcat tacatctgca agagttgata
    16741 tattttgagg agttcttata cctgcaacaa cgtcttcacc ttgtgcattc attaaaaatt
    16801 caccaaataa tttattttct cctgtagctg gatttctagt aaatgcaaca cctgtaccac
    16861 tagtttctcc catattacca aataccattg attgaatatt tactgctgtt cctaagttat
    16921 gagctatatc attcaattta cggtatacga ttgcacgagg attattccat gatctaaata
    16981 cagcctctat tgcaagcatt aattgctctt ttgggtcttg tgggaaatca ttttttattt
    17041 cttttttata aattgcttta aattctttta ctatttcttt taaatcatca gttgtaagtt
    17101 ctgtatcaac tgtgtatccc tttgcttctt ttactctgtc taatacattt tcaaatttat
    17161 attttggaac ttccatagca acatcagaaa acatttgtat gaatcttctg taactatcat
    17221 atgcaaatct ttcattttgt gtagcctctg ctaagccaac aacactattg tcattaagac
    17281 ctaggttaag aattgtatcc atcattcctg gcatagagaa tactgcacca gaacgaactg
    17341 atactaataa tggattctta ttgcatccta atgttttatt taagtctttt tctaaagtag
    17401 ctaaatgctc ttctatttct tttattatct ctgctcttat actttcattg ttaacataat
    17461 aatcattaca tgcttctgtt gttatagtga atccaggagg gacaggtaag cctatcttag
    17521 tcatttctgc taaattggca cctttacctc ctagtaaaga tttcatgtct ttgcttcctt
    17581 caccaaaact ataaacatac ttagtttcca ttgttaaatc ccccattaat ctttttattt
    17641 taatttatat catttttatt aatagacatt attaaatttc tatttctttg tatatcttta
    17701 gaccattttc tttcattatg tctaaaatta tacctgctgt ttcttctatt gccttatttg
    17761 aaacgtttat tactgggcat ccaattcttt tcattatctc ttctgaataa tctaactcct
    17821 gaagtattct ttctaaatta gcatagtttg ctttactgga caatcctaaa gcttttaatc
    17881 tttgggttct tatctcattc agcttttcag gtgaattagt aagacctatt atctttttag
    17941 tatctatctc aaacacttct ttaggaattg gtatttctgg aactaatgga acattagcta
    18001 cttttatatt tttattagct aaatacatac tcaaaggagt tttagatgtt cttgatattc
    18061 ccactaaaat tatgtctgct tgaagaacac ctctagggtc ttttccatca tcatatttca
    18121 ctgcaaattc tatagcttca actctcttaa agtaagattc atctaatttt ctaattatac
    18181 cagcttctct tttaggtttt cttcctgttc tcttgcttat ttctcttagt atatctgtca
    18241 ttaaatctat acaactaaga ccttctcttt cgcaatatct ttcaacaata ctgcataatt
    18301 cttcatcaac taatgtatat acaattaaag cgttttcagc ttttgcattc tccaaagttt
    18361 caagtagtac tcctttatta atcatgtatg gaaatctttt tatttcataa gtttcattca
    18421 cataaaactg agacatacat gcctttgcaa cttgttgtgc agtctcccct actgaatctg
    18481 atactgcata tataattagg tttttcacca gcatacctcc ttaattgtca actatttcta
    18541 agaatagctt agttatgttg gtttttgata ttctcccaac aactctcata tgatttttgt
    18601 cttctccctt atattcaaca atcggaatcg aatctacttc atgttctatt atttttcttg
    18661 aagctaataa aacacttgct cctttagtta aagtgactac atttggagtt cttgtcatta
    18721 tcatacctat tggcatttta tttatatcag aacctcctat agttgctttt agcaagtctt
    18781 ttctggaaac aactccacat aactcttcat tctcatctat aataactata ctccctacat
    18841 cagataaaaa catagtgact attacatcat atatactttc atccttctta gctagtacag
    18901 gcatgctcat tatatcctct actgtttttt cttttacact tttaccaatt aaatttattt
    18961 cactaactcc tgaataaaaa tatccaactt ttggtcttgc gtctaatatt cctgtcattg
    19021 ttaatatagc taaatcagat ctaagtgtag cacgagtcac attcaagctg gaggcaatac
    19081 tttcactcgt tattggttca ttttccttaa ctatatctat aatttttagt tgtctttcat
    19141 taagctgaat aataatcacc ttctttcatt gtgctatact ttttatcttt tcgcatctta
    19201 tatagtatat tatataacta tatataccat tctgtaaaca gattttttat tgaatacgat
    19261 atatttttta ctaagttctc tcgcttaaag atatttatta tatattaatg ggggatattt
    19321 ctttaattaa tagaactata ttagtattac agttaatatt ataatacata attggtcatt
    19381 ttaattcatt ttatattaaa atttttaata atattatttc aatgtgaata taaaattgca
    19441 gaactaattc aaaataattt ttacgactac aaataaccta taatatactt ttaaagaaag
    19501 tatcctaaca agaatatata aaaatttaaa agggttgatt taaaaataac catcaaccct
    19561 ttttactaat atctatttat attaccatca aatcttaccc aaatattttt tcttgcaatt
    19621 gtcttaaatg atataaagta gtatcttttt ctatctctac catatcgtaa gttataactt
    19681 cacctttttt aatatctttt acaactttag tctttttact tatcaaacca ataggtacag
    19741 cattcatagc ctttgctaca tcgtattttt ctatagtacc atagcatgta tatgctccat
    19801 agccatccat aaactcacct tttttcaaat ctttcttagc tatagtcata acttctgcta
    19861 ctagtccagc tttaggtact attgtagcct tatggtctaa aactgccata gctgctgata
    19921 aaggagtctc taaactacat aaatgatatg gtctatataa tatgtaatta ggtccatcac
    19981 ccatacttaa atattttagt tcatcattaa ctgcttttaa tttatgggca actactacga
    20041 aaactccagg tgctatacca tttacaaaat caactacacc ataattatct aatactccac
    20101 cttcgctttt taatgcatat ttcttaggta cttcattaac tgtagcttca ataccatgac
    20161 cacctcttac atcaggcaca aatccagttg cattacacat taaagccatt tctaccattg
    20221 ttttagttcc ttctttaaaa gaagctatca tatgaggtga agctcctttt ctttctgctt
    20281 cttctctaac tgtatcagga ttacaatcta aatctaattt attattttta cctttaccta
    20341 tcactctaac ttcaaacccc attgcatctg caaaatcaaa taattccatt actgcacctg
    20401 gctcatctcc tgcagaacct gtaaatatta ctccagcatc atctgctaat tttttaagta
    20461 ttggtccaac aacacaatca gtttcagcat ttaacataac tatatgtttt ttatttaaaa
    20521 tagagtttaa tgctatctct gcaccaattg caataccccc agttgcatct ataactacat
    20581 ctattgactt agttttacaa gccacaagaa agtcattaca tgtcaatact tttccttctt
    20641 gtaattttat ttctgcatcc ttttcatttg ttacattatc tataatttct tcatctattc
    20701 ctgcatcagt aaaagcttta tgagccaact ccaaatctat atctacaaca atagaagctt
    20761 caataccttt catatttgcc atttggctta ccattccatt tcccatatgc ccagctccta
    20821 taagagctac ttttattttt ttaccttcct cttccaattt tgcaagtttt ctgtttaaat
    20881 ctaacatacc aattcctcct gttataataa ttttattttc aaattaagaa atagttatta
    20941 aatctccaat agttatatct ggatttccta tttccacatg tatttctcct ggtaaattta
    21001 cttcacattt tccatcaaat ttgattgtac aatgcccaat ttctctaaga gtcttctgag
    21061 caatatcacc aactgaaact atacaataat ctctattccc tatagtaagt gtatctccta
    21121 tttcaacatc actttttaat gttgctattg aatgaattac agatatatcc tttaagtcgt
    21181 catcattaat ggtttcatca tatataataa gacaattgct ttcatccatt aactctttaa
    21241 ctaaactgcc tatcccactt atcttaactt catatttcat gacattcctc ctgataattt
    21301 atatttatat tgagttaatt aaaagaaaca tttatttttt cttttataaa ttttatagct
    21361 tgtacaatac tctcttcatc tttaaatttt tgctctgcac tatatatatc taatccttct
    21421 atttcttcaa tctctttaaa tttagccatt actgtacgcc cttttaaaat ttctccaaac
    21481 tttataaacc catctgaatc tgaagatata acggctatac ttcctttaga aaaatagctt
    21541 ttctttttgc ctgtagacac tatatcaccc ttttttttag ctctatttaa agttttaaaa
    21601 tacaatttat tctgcttcat tataaatata gtatttaaaa tataagcaac aaataaaatt
    21661 actaataatt tcatatttat acaccttcca aaaatcacat tatttttaaa tctgtataac
    21721 aactataaat gctgtacaat agttatataa ggtttttaag taacaccttt ttaaattcta
    21781 aaaaagttct acatttactt atcttattcg ttaattttac atcttggcct atctttaaaa
    21841 cttcttcata aatttttact aataagtctg agtctatctt attctcacat ggaaacattg
    21901 tcataagaat tatttttata cttctatttg catattctat tggttcttct aaaattccaa
    21961 ctgccattaa aaacttatct gatttttcat ttatagaatg aggaaataat aaacctttat
    22021 caaatactgt gggagatttt tcttctctat tgactatatt ttttctaaac ttctcatcaa
    22081 tacatcccaa atccataaga tggtccatca ttttttctaa tgaggcaagt atagtctttt
    22141 catttaatat cataaattta tcttcatcta acaaattatt tattagtaaa tttgtagaat
    22201 tagtagtgtc aatattccct tctcttaggc acaatactgt ttttaattgt tctcttagtt
    22261 tgttttcatc aaatattgta tttattttta atactattgt atttatattt gatgtgtcta
    22321 aatcaatagt tgtaaaaact atatcgtatt catctaagtt aatatttctc aaattaaaac
    22381 ttgaaagagt atcaatttta ggattatttc ctatcagttt ttctactctt attttcaata
    22441 gttttgctgt tcctagacct gtactacata taattgctat tttttttatt gaatagaatc
    22501 tactgctatt tctttctaaa tatccactga aatggattgc tatatatcca atctcatctt
    22561 ctgaaacttc taagttatac tttttctcta ttacttttcc tgctatttgt gctaatttaa
    22621 atggaagtat ataattttgc ttaatctctt ctaacaaaac attttttaac tttatattaa
    22681 ataatagcct gtttaaagta aaactaagat gatatcctag acctactctt aattcatcct
    22741 catttataaa aatacccata tcgacatata tttgattaaa tatattttcc atcaactcat
    22801 caatgttttt actaattcta ctactattta aagcacattc tgatgctggt gcatttccac
    22861 taactaacgg tatagttaaa taaataattt cttcatcatt taaagtacat tcaaacattc
    22921 ctgatattat atgttttatt tccctagcaa taaagtattc atcatatgat ttaagtaaat
    22981 caaactttac atcataatct tttactttct tttcttcttt tactctatag actaaaacta
    23041 ctatgtagtt aataacttct cttaacattt catctgtaac ataaaaatta ttttttttaa
    23101 ataaagtcaa tagtttttct tctaaattta tgatttcttc ttttttgatt cctttaaaat
    23161 actttgaatt actgaaatct ttccttgagt ttttatacaa ctgatttaat ataaacagtc
    23221 taatatcaag ttcatttcca tttaatttca tacctgtatt ctgtttttta atcaagttta
    23281 aattatatga agccaaaact ttttctacat actggaaatc atttactaaa gtagttcttc
    23341 ctacacacat ctcatcagct aaactatcaa gtgtaataaa tccatctagt tctatcaagt
    23401 aatttagtat aaactctact ctatcttctt ttgaatttaa tgaaaataca ttttttctat
    23461 tcatatcaat aatcttattg agtatctgct catcttttat ttctagttta tagccttgac
    23521 cctttatata tttgattact gctatatcat tacctaaatc tttattcaat tgttttatat
    23581 acgtccttac acttctagtt gtgacattca attcatttgc tattgagtta atactcacaa
    23641 actctttttt actacacata atcaatatct taaatatctt gcggtcaata gaaggaattt
    23701 tctcagttac actcattttg tatctcctcc ttgatttcat tctagtacaa atttgaaacc
    23761 aaattaatac ataaaatttc actttaataa aggaaaattt agtattaatc taactcgata
    23821 attacagcta attaatagta taaaagctag tctttcatta acaaaaaatc ttgactagct
    23881 taaatttata tttacaattt tattaattaa ttccaagcct tatattgaca ttaaatatta
    23941 tctcaatgca aatactgcaa ctccatatgc aataataaca gctattggac cagttattaa
    24001 acgagagtaa aataaagctg gaacaccata ctctacagtt tctggttctg cttctcccaa
    24061 acttaatcct actggtacaa aatcacatcc agcttgccca tttattgcaa ataaagctgg
    24121 aagtgctaaa tatgctggta ttgcaccaag accaatttgt gtacctacaa gagtccctac
    24181 tacttgagct ataactgcac caggtcctag tattggtgat aagaatggaa gtgtgcaaat
    24241 tactgatata atcaataatc caccaatatt acctgcaagt ggagatataa ctcttgcaat
    24301 ccagtctccc agtccagatg ctaaaataat tcccataagc atacttacaa atgccataaa
    24361 tggtattacg ttattcatta ccatttggat agtatctcga cctgcatcat aaaattttga
    24421 tactacttgc cctgcccctt gaccaataga aagtattatg ttctttttag catattcacc
    24481 ttgtgctttt gacttaacct catcataatt atcaggcttt ctaatagaac tcttattttc
    24541 gcttttattt tcttgagatt tttgtggcat atcttcccca tctacaacac ttatacagtt
    24601 aggattaaca tctgatacat aatattcttc tgttataaat tttgctaaag gacctgtttt
    24661 tccaactgga tttacattta ttgtaggtat ttttttcttt ggataaactc cacatctagc
    24721 agttccacca caatcaataa taactactgc tacttcttca tcagaaactc cagttttaaa
    24781 tccatctaca atttcacaac ccagtagttc ttttattttt aatgttactt catggatacc
    24841 gccagcagtc attgatagta tcttatttct attcccttct ttttttatat acaaaggacc
    24901 tccccagcct gaagttccct tttctatttt taatatttta ttctcctcca ttttatctac
    24961 ctcctaagcc ttatttgttg ctctattttt tcttgttaga aaagcataca ttttttctgt
    25021 tactatacct cttataaaaa ttacaattag accaactata aaataccaca atgccaatgt
    25081 agtagtttct ttacctaaag tagtcaaacc tgcagatatt cccaaccata caaaaagttc
    25141 tcctgcattt gcatgtggaa aaagacctgt aattggatgt acaaatgaaa ctgctgcatc
    25201 ataaaatcct gctttgtgct cttcttttaa aaatctacca aatgtatagc acataggatt
    25261 tcccaagaaa aatactgata ctaatggaag tagaatatat cttgtaaaag agttctttcc
    25321 tagcttagaa gcccaattat taactctttc ctcaccaatt atcttaatca aagagttaat
    25381 agctaccatt aatacaatta gcgttggaag agtacctgaa acaagaccta taaattgttc
    25441 tccccctttc tgaaacaaac cgataaacca ctctgcacct ttacttagac ctgtaataac
    25501 gttttccata aaattataac ctccctataa tatctttaaa tttttaattt tactgcttac
    25561 cataaatttt acaaattaca tcttttttct gtactatatg ctaatattgt aaatgttttt
//
LOCUS       DAMJYR010000047        25387 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.047, whole genome shotgun sequence.
ACCESSION   DAMJYR010000047 DAMJYR010000000
VERSION     DAMJYR010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 25387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25387
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.047"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(253..969)
                     /gene="dapD"
                     /locus_tag="PC471_001898"
     CDS             complement(253..969)
                     /gene="dapD"
                     /locus_tag="PC471_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HDF2789028.1"
                     /translation="MVNLNDAYEIARYIKEVKKKTPVKVYVNTNSLHFQSTDKFKVFG
                     SNGSYIFVGDYEDIMEVLDKHGNDITDTHVEYDRRNSAIPLKNTLSEHARIEPGAIIR
                     DMVTIEKNAVVMMGAVINIGAVIGEGSMVDMNAVIGARGTLGKNVHLGAGAVVAGVLE
                     PPSATPVIVEDDVLIGANAVILEGVRIGKGAVVAAGAVVTTDVEAGAVVAGSPAKVIK
                     MKDEKTADKTKLMEDLRNLD"
     gene            complement(1193..1942)
                     /locus_tag="PC471_001899"
     CDS             complement(1193..1942)
                     /locus_tag="PC471_001899"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HDF2789029.1"
                     /translation="MLKVIATGYDGKMGKILADTIREDNELELVCVAARGLDSYEGDL
                     KIYEDMSTIVEEADVVIDFSHHSNLDNILSYVLKTKTPLVIATTGYNDDEMNKIREAA
                     KVIPVFQSYNMSLGVNVLVKLVKDAAKLLEGFDIEIIEKHHNRKVDAPSGTAVMIANA
                     IKEVLPNVENNYGRYGRSAKRQPNEVGIHAIRGGNIVGEHDALFAGDNEVLTISHQAQ
                     SRGIFASGSIAAAKYLVKQKPGFYNMDSMLG"
     gene            complement(2009..2896)
                     /locus_tag="PC471_001900"
     CDS             complement(2009..2896)
                     /locus_tag="PC471_001900"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HDF2789030.1"
                     /translation="MIFKGSAVALVTPFTKDNKVDFDKLGELVEYQIANGTDAIVSCG
                     TTGEANTMTDEEQLATIKYVVEKVNKRVPVIAGSGSNDTMHSVNLSQEAEKLGVDALL
                     IITPYYNKANKAGLKRHFETIANSVKLPIILYNVPGRTCVNISPSLIVELAKIDNIVA
                     VKEASGDLGQVAEIASLVPDDFAIYSGNDDTILPLLSLGGQGVISVLANVCPQETHDL
                     VAKFFEGDIEGSRKLQLGMDALIAALFIEVNPIPVKTAMNLLGFNVGDLRLPLAEMDP
                     ANLEVLKKELVNFGLKVNA"
     gene            complement(3013..4014)
                     /locus_tag="PC471_001901"
     CDS             complement(3013..4014)
                     /locus_tag="PC471_001901"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HDF2789031.1"
                     /translation="MKKVNVAIVGATGMVGRTFLKVLEERNFPIENLFLFSSSKSAGS
                     KVMFCGKEYIVEELKETSFTDRGIQIALFSAGGSISEKYAPIAASNGILVVDNSSQWR
                     MNPDVPLVVPEVNPEAIKNHKGIIANPNCSTIQAMVPLKALDDKYKIKRVVYSTYQAV
                     SGSGVKGVEDLEKGMNGNPTGFYPHQISYNCLPHIDSFTENGYTKEEMKMVNETMKIL
                     DNYDLKITATTVRVPVKNGHSESINVEFNNPFELDDLVKTLEEAPGVVVVDNPAKNEY
                     PTAVEFDDRDEVFVGRIRRDFSVDNGVNLWVVADNIRKGAATNAIQIAEMALSYDLV"
     gene            complement(4439..5320)
                     /locus_tag="PC471_001902"
     CDS             complement(4439..5320)
                     /locus_tag="PC471_001902"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HDF2789032.1"
                     /translation="MLFKGSAVALVTPFTEDNNVNFEKLGELIEYHIENGTDALVVCG
                     TTGEATTMSESEIFAVIKYTVEKVNKRIPVIAGTGSNNTMLSVHMSQEAEKLGVDGLL
                     IITPYYNKTNEKGLKLHFETIANSVKLPIILYNVPGRTKVNIKPSVVAELAKIDNIVA
                     VKEASGDLAQVAEIAKLVPKDFAIYSGNDDTILPLLSLGGSGVISVLANICPKETHDL
                     VTKFFEGDIEGSKKLQLDMDALIAALFIEVNPVPVKTAMNILGFNVGDLRLPLAEMEE
                     TNLNVLKQELTNFGFKF"
     gene            5643..6836
                     /locus_tag="PC471_001903"
     CDS             5643..6836
                     /locus_tag="PC471_001903"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HDF2789033.1"
                     /translation="MDTLKELFKIGSGPSSSHTMGPQRAAERFKNENPDAESFRAILY
                     GSLAATGKGHLTDYIIEKTIAPKKVEIVWEEDIIKDFHPNGMKFEALDKDKNVTAEWT
                     VYSVGGGTIAEEGQRNSKSNSIYHLDTMDEIVKWCKENNKTLVDFVLECEPKDIKDYI
                     KTIKDAMRKSIDDGLSTDEIIPGKLLLKRRASTFYNAYKKDKSFSTLVYAYALAASEQ
                     NASGNIIVTAPTCGSAGVIPGIFFAMQDFYNYDDEKIIEALLVAGIIGNIIKTNASIS
                     GAEVGCQGEVGAACSMAAAAVAYLKGGTIDHIEYAAEIALEHHLGMTCDPVYGYVQIP
                     CIERNAMAAQRAYDAANYALLTDGSHSVSLDQVVETMKETGIDMMDKYKETAKGGLAK
                     HFFSC"
     gene            7042..8160
                     /locus_tag="PC471_001904"
     CDS             7042..8160
                     /locus_tag="PC471_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyldiaminopimelate deacetylase"
                     /protein_id="HDF2789034.1"
                     /translation="MREASYLQEQLYKHRKSLNPIAEIGRKEYKTSKYIRDYLDKLGI
                     EYEVYLETGIVGVIEGKRPKKDIAFRADIDGLHSEEGVKHLCGHDGHATILLGLIEYL
                     NDNKEDLNDNIVFIFQPAEEGPGGAKALIDEGVLRKYNIDEIYGLHIYPELPEGYVGT
                     REGYFMAQIGDIDIEIVSKSGHGAMPQNGIDGIVIASNFVSSLQTIVSRNISPIDNAV
                     LTIGRIEGGARRNIIAENIKMEGTMRCFSPEVYEKMKLRVRELARGFELAYNCKVNIN
                     IIDDYIAVKNDKDLYQEFVEAIGSDTVVELEPLMISEDFSYYQKEVPGLFFMLGSRNE
                     EKGFVNGLHNINFNFDEKICVNALNVYSKLLKYKEAID"
     gene            8291..8536
                     /locus_tag="PC471_001905"
     CDS             8291..8536
                     /locus_tag="PC471_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small, acid-soluble spore protein, alpha/beta
                     type"
                     /protein_id="HDF2789035.1"
                     /translation="MKEKVQREKKITNGKNTKILTNDDIMKYEIASELGLMDKVGERG
                     WAGLTAKEAGRIGGMLTSRKKRLKKIVEEQNKKDESI"
     gene            8523..9269
                     /locus_tag="PC471_001906"
     CDS             8523..9269
                     /locus_tag="PC471_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HDF2789036.1"
                     /translation="MSQYSDFAFVYDELMNEVDYNGWVKYIEDIIDSEGVKVKNILEL
                     ACGTGNLTIPLTKKNYDIAGIDISDEMLSVAREKAEKEGVELVLLQQDISELDFEISD
                     LDCVLCACDGFNYITYDDDLENVFSKTYELLKKDGIFIFDISSFYKLANILGNNMYGE
                     NREDIAYMWQNYFDEEENLIEMELTFFIKDECGKFERFEEIHQQRAYTEDEIVDMLEK
                     SGFKDIKYYGDFTFDKPSEESQRIFFVCKK"
     gene            9554..10429
                     /gene="hslO"
                     /locus_tag="PC471_001907"
     CDS             9554..10429
                     /gene="hslO"
                     /locus_tag="PC471_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HDF2789037.1"
                     /translation="MRDYVLRATSGNGQVRAFVATTRNTVEEARRLHETTKVATAALG
                     RTLTATSIMGLMMKNDSDKLTVIIKGGGPIGTIIATSDSKGMVKGYVGNPQVEVEDYP
                     NGKLNVAAAVGTEGVVKVIKDLGLREPYNGTYPLVSGEIAEDFTYYFAVSEQTPSVVA
                     LGVLTKEDEVEFAGGFIVQLMPDAEEETIAKLEENVAKLPSITNMLKEGKSPEDILNI
                     VLDGLEPKILDTCEVGFMCECSKERVKTALVAIGKKSLAQIIEEDKKAEVGCQFCNKK
                     YMYSEEELLEILKEM"
     gene            10820..11620
                     /locus_tag="PC471_001908"
     CDS             10820..11620
                     /locus_tag="PC471_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HDF2789038.1"
                     /translation="MESKTKYIGISILILLMIFLIAKNFNGKLEIPIPESNSRKFKSA
                     AVFYPQHQDDEVLWGGSAIVDAIKQCGVDNVYVVLVSDGSGVNVFKANTKFRNLTRKQ
                     KEELRNNEFKSALRELGVKPQNVIILADIDKKEGTHYELMEKTILDFEHKFKGNVTHI
                     AHHYKYDDHIMHRKNGEVLKKLKREGKIKDDLYFVKPKYVKYIPRNERAIYKAMDFDD
                     YNKVKSACYEYKVVNSKRKMYGIGYISAHSYFEHLLNDPELTSVLSED"
     gene            12148..14388
                     /locus_tag="PC471_001909"
     CDS             12148..14388
                     /locus_tag="PC471_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HDF2789039.1"
                     /translation="MNNEFIILKGCKENNLNNISLKIPKRKITIFTGVSGSGKSSIVF
                     ETIAKESQRQLNERFSTFVRSFLPKYGEVKADCIENLSTPIIIDQSRLGGNSRSTLGT
                     ITDVNSFLRALYSRFGSEYIGNANMFSFNNIDGMCPHCHGLGKKLVPNMNQILDMNKS
                     LNEGAILLSGFGVGSWHWNIFAESGFFDINKRICDYSEVELEKFLHGKAEKIKIENAG
                     QTNMTYEGLMVKFNRLYLSREGEISEATKKKLSKLLIEDRCPICDGRRLNERVYRSLI
                     NGYNIADLTSMQIDELAEVIKSIDEPEAQPLIKGIIEKLNSIIDIGLGYLTLDRETSS
                     LSGGESQRIKMVKYLNSNLVDLMYIFDEPSVGLHPKDVYKLNNLLKKLRDKGNTIIVV
                     EHDPDVIKIADHIVDVGPKAGKYGGEVVYEGSYENLLTSGTLTGNALSKFLPIKESVR
                     EHSGYLEVKNCNKNNLKNVSIKIPKGVLTLVTGVAGAGKSTLIKDEFLKQNPSAVLID
                     QSPVSANSRSSLATYSGIMNNIRSIFSKTNGVNASLFSSNSEGACDNCKGSGIVEMNL
                     AFMESIKSTCNICEGKKFKKEVLEYRFQGKNIIEVLEMSVLEAIEFFNLKQIKTKLQC
                     IEKMGIGYLTLGQTLDTLSGGECQRLKLASELNKESSVYILDEPTTGLHMADIENFIS
                     IIEEIVDNGNTVIIIEHNIDVIKRADWIIELGPEGGTKGGRVIFEGIPKQLCNNKISL
                     TAKYIV"
     gene            14802..16337
                     /locus_tag="PC471_001910"
     CDS             14802..16337
                     /locus_tag="PC471_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HDF2789040.1"
                     /translation="MINDILTLLSERSDFFIKLSIEHLMISIISIIIAIVLGGIIGII
                     VSEFHNSSKPILGTINFLYTIPSISMLGFLIPFSGIGNTTAVIALTIYALLPMVRNTY
                     TGITNVDKNILEAARGMGSTDFQILYKIKLPLALPVIMSGIRNMVIMTIALAGIASFI
                     GAGGLGVAIYRGITTNNTIMTITGSILIALLALIFDFILGLVEKFFQRKRKKKNRKKV
                     FAILGSLILIVIIFTSYLFNPSKTQTIHIATKPMTEQYILGEMLDILIEKNTGLNVEI
                     TQGVGGGTSNIQPAMENGEFDIYPEYTGTGWNMVLKKNELYTESMFSKLQSEYKTKLK
                     MQWTGMYGFSNSYALAVRKEVADKYNLKNITDLKDVSSKLTFGAEYDFYEREDGYTPL
                     CNTYGLNFKDTVDLDIGLKYQAINEGKIDVMPIFTTDGQYSVSDVVVLKDDKNFYPSY
                     QCGNVVRLEVLEENPELSKVFKMVGDILTDKEMAKMNYDVENNNLEPREVAREFLNKK
                     GLL"
     gene            16353..17114
                     /locus_tag="PC471_001911"
     CDS             16353..17114
                     /locus_tag="PC471_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2789041.1"
                     /translation="MTPIIQFKNIKKQYNDKTIIDNLNLDIEKGEFLTVIGSSGSGKT
                     TLLKMINGLILPDGGNILINQTDIKNEDLIKLRRRIGYCVQGSVLFPHMTVEENISYV
                     PNLLSKKNKLEVKSAVNKWMEIVGLPNDMKVRYPSELSGGQQQRVGIARALASSPEIL
                     LMDEPFGAVDEITRKQLQKEIKEIHKKTGVTIIFITHDIYEALILGTKTLVLNHGVVQ
                     QYDTPENILNTPANQFVDQLLNIRKSIIDDDNLKE"
     gene            17452..18321
                     /locus_tag="PC471_001912"
     CDS             17452..18321
                     /locus_tag="PC471_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HDF2789042.1"
                     /translation="MDNNLITIEKTLDYIEKHLLDEMLDLNVISKEIGYSKYHLHRMF
                     TSVVGFSVHNYIQRRRLTEAARMLIFTEKPIIEIALISGYDTQRSFSRGFKKMFKRSP
                     NFFRRHQDFLPLQLKFDIHNRKKLRGDMVLDVKKVNCADINIVGYSGKVRKGFHIIGK
                     CWRDLHKNKEMIENKVDLGFLIGVSDYSNFKEIENTPTFTYIAGAQVATFGKLPNGME
                     KFVLPKSNYIVFTFIGKNEDSMQNVVEYIYQEWFPNSSCQFNENNLYDFVKYGEKTDE
                     NGLSEIQFWVPII"
     gene            18650..18901
                     /locus_tag="PC471_001913"
     CDS             18650..18901
                     /locus_tag="PC471_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3781 domain-containing protein"
                     /protein_id="HDF2789043.1"
                     /translation="MNVENELLKNLDRLHTTELGVVRIKKNLSLETNDVVNWCKTKIE
                     SPNAIINRKGKNWYISVYDCIITVNAHSYTIITAHKEKK"
     gene            19013..19792
                     /locus_tag="PC471_001914"
     CDS             19013..19792
                     /locus_tag="PC471_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HDF2789044.1"
                     /translation="MNDTVKEKLAESLTSDTTDILPYLPYLLQDFWELGSSPEQMLQL
                     FRKHIPFNEDTKVLDLACGKGAVSVKLAKELNIHIKGIDLIPEFINYARRKAIEYKVD
                     ALCDFEMNDANSSVKIEHDYDCVIFGAVGDILGNYKETISKLLPTIKSKGFILIDDAY
                     VVDKSTNEQLNFERVYPTYNEWCILFKELGLKLIECIPCLELENDVEEEMNWIITRAN
                     ELIKKYPSQKDMFEQYVISQKNEYTDLQQDLVGATWILQKE"
     gene            complement(19927..20577)
                     /locus_tag="PC471_001915"
     CDS             complement(19927..20577)
                     /locus_tag="PC471_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2789045.1"
                     /translation="MNIVMINGSPKVNKNNSQYFLSELKQLIQNDNKVFTFKVDSTSK
                     YPDALNAILNCDRLIIASPLYVDGIPSHLLHFLEKCEKFLKTQNKKPIPVYAIVNCGF
                     NEGEHNHLAIQMIEHWCKKINFTWVQGIGIGAGEMFGSLESVPLGKGPKKDLGIALKE
                     LSKNILSDNIHIDDSNKSTIFISPNFPRFLYKLMGNHQWNTQAKSNGLTKSDILKK"
     gene            complement(20574..21077)
                     /locus_tag="PC471_001916"
     CDS             complement(20574..21077)
                     /locus_tag="PC471_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HDF2789046.1"
                     /translation="MRLLIHDLTDKELEKLLPKLDDNIKILPNTKEIHKCIGCFGCWI
                     KTPSECVVKDYYSNMGKLINDSDGIIIISRCCYGGFSPFVKNVLDKSINYILPYFVIK
                     DNMMRHKSRYDKQINLKAIFYGDCITEAEKETATKLVNGNAINFNIKEHSVCFYENIQ
                     DIRGESI"
     gene            complement(21126..21641)
                     /locus_tag="PC471_001917"
     CDS             complement(21126..21641)
                     /locus_tag="PC471_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HDF2789047.1"
                     /translation="MLEYIILGFLMEKELSGYDLKQIMSESTSYFFDASFGSIYPALK
                     RLETKGYIHYHEVIDGSKLKKLYSITNTGKEVFLEWLKKPINFSKTKQDHLVNIFFYK
                     YLPKEIIETNLKMLITEVELLLNKLSKQKNDAEENHCVDQYTYMYATMIYGVDYYNFV
                     INWCKDLLKNL"
     gene            21921..22448
                     /locus_tag="PC471_001918"
     CDS             21921..22448
                     /locus_tag="PC471_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDF2789048.1"
                     /translation="MHEQIDYCERKKIRTIDIKGENMRELLKWVSIINRYNTNYIDRK
                     LADIGINNSQYFYVLHICNNPGITQDTIFEKILVNPSNITRALANLEKEGFITREPGV
                     KDKRTWHLYPTEKSLGCYDEILKITNGYVEELLSSFEKEEQELFMSMLKKVAFLAIDM
                     NEEAKLEAKENGRKI"
     gene            22432..23835
                     /locus_tag="PC471_001919"
     CDS             22432..23835
                     /locus_tag="PC471_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HDF2789049.1"
                     /translation="MEEKYKEPLEYEKVSKLLTKYAIPSIIAMLVSALYNIVDQIFIG
                     QGVGVLGNAATNVTFPLTTICTAIALLLGIGGASKCSLELGAKNSEKATKAAGNSICL
                     MAVFGISLFIVVSIFLTPLLKFFGSTSEILPYAHTYTKITSIGLPFLIMSTGMSKLIL
                     ADGRPKASMFCMLIGAVINTILNPLFIFVFNMGIAGSALATVIGQLISFGISVHYIKN
                     FQHIKLHKKSFKLDSIIYKHIFALGSSSCFNQLAMTVTQIVMNNTLAYYGARSVYGSE
                     IPLACVGIIIKVNMIFISIIIGISQGMQPIIGYNYGAEKYNRVKEAYKFAVGSATIIS
                     VIAFLCFQFFPRQITSIFGNGNETYFIFAEHYFRTFLFLTFINGVQIVSSTFFTSIGK
                     SVLGLFTSLTRQILFLIPLIVILPMFMGIDGVMFAGPIADAAAAMVCIYFAVHEMKSL
                     TIKQNNLMSNHNKIAEV"
     gene            complement(24218..24529)
                     /locus_tag="PC471_001920"
     CDS             complement(24218..24529)
                     /locus_tag="PC471_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2789050.1"
                     /translation="MKKIIKSFTSWFFLIALFEIYMHQIGQDSKSIVLIGLNPILSII
                     SRVDSFFVFMDSGMQIPCRTITGSISIYWYIASILSFLIYGIILDLIRIVISKIGNKT
                     K"
     gene            complement(24585..25178)
                     /locus_tag="PC471_001921"
     CDS             complement(24585..25178)
                     /locus_tag="PC471_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HDF2789051.1"
                     /translation="MISDSISKRRSIRKYKNQSISHETIEKIIEAGINAPSSKNRQPW
                     RFVVITEKEKESMLKAMSKGIQNEINDNGLLPGSRQHIAGANYTVEIMKQAPVTIFIL
                     NILGKSPLEKLSPEERFYEMANMQSIGAAIQNMSLTAVELGLGSLWICDVYFAYRELC
                     EWLNTDSQLVAAISLGYPDEEPSRRPRLQLSDVTEWR"
BASE COUNT         9405 a         3135 c         4188 g         8659 t
ORIGIN      
        1 aaaaagttta atagtttatt acattgatat attgaaaaat tgaaggaaat aaaaatactt
       61 gtcagcaaga aataaaaatg atataagaat gaataagaga attgtaaaaa aataaaatga
      121 ttataaataa aagataaaaa tattgtaaaa aagagggtgc ctctggtgaa ttaaattcac
      181 acagagacac ctttttaatt aattttttaa attagtatat tattgtttaa ttaatagtat
      241 agtactattt aattaatcta aatttctaag gtcttccata agcttagttt tgtcagccgt
      301 tttttcatct ttcattttta taactttagc aggagaacca gcaacaacgg caccagcttc
      361 tacatcagta gttacaacag caccagctgc aacaacagca cctttaccga ttctaacacc
      421 ttcaagaata actgcattag ctcctattaa aacatcatct tcaactataa caggagtagc
      481 agaaggtggc tctaaaacac cagcaacaac agcaccagca cctaagtgaa catttttacc
      541 aagagttcct ctagctccaa taacagcatt catatctacc atagaacctt caccaataac
      601 agctcctata tttataacag ctcccatcat tacaacagca tttttttcta tagtaaccat
      661 gtctcttatt attgcacctg gctcaattct tgcatgttca cttaaagtat tttttagagg
      721 aatagcagaa tttcttctat catattctac atgagtatct gttatatcat ttccatgttt
      781 atctaatact tccattatat cctcatagtc acctacaaat atataagaac cattacttcc
      841 gaatacctta aatttatcag ttgattggaa gtgtaaagag ttagtattta catatacctt
      901 aacaggtgtt ttctttttaa cttcttttat ataccttgct atttcataag catcgtttaa
      961 atttaccatt tgttaattct ccttgtattt cattatattt cttttattat atacttattt
     1021 gtcaatttta gtatatactt acttcttatt atttatattt tattttagtt agtttttatt
     1081 gtatagacat agtattaaat aaatttatta tatatgtttg atttgtattg cgtattatta
     1141 tatctatatt gcatatgttt aatttatatt gtgtattatt atatttatgc tattatccca
     1201 acatgctgtc catattataa aatcctggtt tttgcttaac taagtatttg gcagcagcta
     1261 tagaaccact tgcaaagata ccacgagatt gagcttgatg gcttattgta agaacctcat
     1321 tgtctccagc aaatagagca tcatgttcgc caacaatatt tccacctctt atagcatgaa
     1381 taccaacttc atttggttgt ctttttgcac tacgaccata tctaccataa ttattttcaa
     1441 cgtttggcaa aacctctttt atagcgttag caatcataac agctgtgcca cttggggcat
     1501 ctacttttct gttgtgatgt ttttctatta tttctatatc aaatccttct agtaatttag
     1561 ctgcatcttt aaccaatttt actaatacat ttacaccaag agacatatta tatgattgga
     1621 atacaggaat aactttagcc gcttcacgta ttttgttcat ttcgtcatca ttatatccag
     1681 tagtagcaat tactaaaggt gttttagtct ttaaaacgta agataagata ttatctaaat
     1741 tagagtgatg agaaaaatct ataactacat cagcttcctc aactatagta ctcatatctt
     1801 cataaatttt aaggtcgcct tcatagctat ccagacctct agctgcaaca caaaccaatt
     1861 ctaattcatt atcttctctt atagtatcgg ctaaaatctt acccattttt ccatcataac
     1921 cagttgctat aacttttaac ataatttcct cctatgatta agggctaact tttgaccaaa
     1981 cttacttttt accatgcatt aatctttact atgcattaac ttttagccca aagttaacta
     2041 attctttctt taatacttct aaatttgctg ggtccatttc tgctagaggt aatcttaaat
     2101 ctccaacatt gaatcccaat aaattcattg cagtcttaac tggtatagga tttacttcta
     2161 taaataaagc tgcgattaga gcatccatac caagttgtaa ttttctagaa ccttcaatat
     2221 cgccttcaaa gaatttagct accaaatcgt gagtttcttg agggcaaaca ttagcaagta
     2281 ctgatataac tccttgacct cctaaagata ataaaggaag tatagtatca tcattaccag
     2341 agtagatagc aaagtcatct ggaactaaac tagctatttc tgcaacctgt cctaggtctc
     2401 cactagcttc ttttacagca actatgttat caattttagc aagttcaact ataaggctag
     2461 gacttatatt tacacaagtt cttccaggga cattgtaaag tataattgga agtttaacac
     2521 tgttagctat tgtttcaaaa tgacgcttaa gtccagcttt attagcttta ttgtaataag
     2581 gtgttattat taatagagca tctactccta atttttcagc ttcttgactt aagttaactg
     2641 agtgcatagt atcattagaa ccacttccag ctataacagg aactctttta ttaacttttt
     2701 caacaacata cttaatagta gcaagttgtt cttcatcagt catagtatta gcttcaccag
     2761 tagtaccaca agatactata gcatcagtac cattagctat ttggtattca actaattcac
     2821 ctaatttgtc aaaatcaact ttgttatcct ttgtaaatgg tgtaactaga gcaacagcag
     2881 aacctttaaa tatcatataa aatcaatccc ctctttttta tttttgtgtt ttatattaca
     2941 gttacttcta atgcttgttt ttgagttact attaatattt tacaaaatta tatatttata
     3001 caagtttgta tattatacta aatcgtatga taaagccatt tcagctattt gaattgcgtt
     3061 agttgcagca ccttttctta tattatcagc aacaacccaa agatttacac cattatcaac
     3121 actaaaatct cttcttattc ttccaacaaa cacttcatct ctatcatcaa attctacagc
     3181 agttggatat tcgtttttag caggattatc aactactaca acacctggag cttcttcaag
     3241 tgtttttact aaatcatcta actcaaatgg attgttaaac tcaacattta tagattcact
     3301 atgaccattt tttactggaa ctcttacagt agttgcagtt atttttaaat cgtagttatc
     3361 aagtattttc atagtttcat ttaccatttt catttcttct ttagtgtatc cattttcagt
     3421 gaatgaatct atatgaggaa gacagttgta agagatttga tgagggtaga atccagtagg
     3481 attaccgttc attcctttct ctaaatcttc aacaccttta acaccagaac cagatacagc
     3541 ttggtaagta gagtaaacaa ctctcttaat tttgtattta tcatcaagag ctttaagggg
     3601 aaccatagct tgtatagtag agcagttagg attagctatt atacccttat gattttttat
     3661 ggcttctgga ttaacctcag gaactactaa aggcacatct ggattcattc tccattgact
     3721 agagttatct actactaaga ttccatttga agcagctatt ggagcatact tttcactaat
     3781 acttccacca gcagagaata atgctatttg tatgcctcta tcagtaaatg aagtttcctt
     3841 taattcttct actatatatt ctttaccaca aaacattact ttagaaccag ctgatttaga
     3901 agaagagaat aaaaatagat tttctattgg aaaatttctt tcttctaata cttttaaaaa
     3961 tgttctacct accattccag tagcaccaac tattgctaca tttacttttt tcatataaat
     4021 tcaccccaat tttaaattat taaaacatta ttttatttta gttatacaag taagatatgt
     4081 taatattttt atatatccaa aaactttaat attatattaa caactttaat tatatacaaa
     4141 aaacgtacaa aggtatactt tgtacgtcta atttatcact caaagtaaac cctgtagctc
     4201 atcacctaca aaaataagtg acagtattac acatgttatg tgcaatccca gaaaatatta
     4261 ttgcaagaaa tattttcttc ggctgtaaat cctttttcca atcattaaca cctgccagct
     4321 actattggat actcttagtt acagctacct ctaccctagg ttaataggat attaaaattt
     4381 gattattcta ataataatac atatttaaga aaaaatcaac ttatttatat aaatattgct
     4441 aaaatttaaa tccaaaattt gtcaattctt gttttaacac attaagatta gtttcttcca
     4501 tttcagctag aggaagtctt aaatcaccaa cattaaaacc taaaatgttc atggcagttt
     4561 taacaggaac tggatttact tctataaata aagcagcaat taaagcatcc atatcaagtt
     4621 gtaatttttt tgagccttca atatcacctt caaagaattt agttactaaa tcatgtgttt
     4681 ctttaggaca tatgttagca agtacagaga taacaccact tccacctaat gataatagtg
     4741 gtagtatagt atcatcatta ccggagtata tagcaaagtc tttaggtact aatttagcta
     4801 tttctgcaac ttgagctaag tctccacttg cttccttcac agcaactata ttatctattt
     4861 tagcaagttc agccacaaca gatggcttta tattcacttt agttcttcct ggtacgttgt
     4921 ataaaataat tggcaactta acgctatttg ctattgtttc aaagtgaagt ttaagacctt
     4981 tctcattagt cttattatag taaggagtta ttattaaaag accatccacc cctaattttt
     5041 cagcttcttg actcatatga acagacaaca tagtattgtt tgaaccagta cctgctataa
     5101 caggtattct cttattaact ttttcaactg tatattttat tacagcaaaa atttcagatt
     5161 cactcatagt agtcgcctca cctgttgttc cacaaacaac tagtgcatct gtgccattct
     5221 caatgtgata ttcgattaat tcacctaatt tttcaaagtt gacattatta tcctctgtaa
     5281 atggggtaac taaagcaaca gcagaacctt taaataacat ataatcaaat ctcctttttt
     5341 atctaaattt aattttttaa gtacaataat aattatagta caaattaatt aattttaata
     5401 ggtatagtta atatcttaac tgtttaaata agatatttat attatattaa tattagaaaa
     5461 attttaaata aatgttttat tctcaaaata tgcatattaa tttaataaaa tataccaaaa
     5521 tggaaataaa aaataaattt tatgagaatt taataactaa taattaatat atttaggtta
     5581 taataaatat gtgcgtaaaa tctaacaata agcaataata ccgtaaattt aggaggaata
     5641 atatggatac tttaaaggaa ctttttaaaa taggaagtgg accttcaagt tctcatacta
     5701 tgggaccaca aagagcagca gagagattta agaatgaaaa cccagatgca gaaagtttta
     5761 gagcaatatt atatggtagt ttggcagcta cagggaaagg tcacttaact gactatataa
     5821 ttgaaaaaac tatagcacct aaaaaagtcg aaattgtttg ggaagaagat attataaaag
     5881 attttcaccc aaatgggatg aagtttgaag ctttagataa agataaaaat gtaacagctg
     5941 aatggactgt atattctgta ggtggaggga ctatagcaga agaaggtcaa agaaacagta
     6001 aaagcaatag tatatatcac ttagatacta tggatgagat agtaaaatgg tgcaaagaaa
     6061 ataataagac tttggtagat tttgtgttgg aatgtgaacc aaaagacata aaagattata
     6121 taaaaactat aaaagatgct atgagaaaat ctattgatga tggactaagt acagatgaaa
     6181 taataccagg caagttatta ttaaaaagaa gagcaagtac attttataat gcatataaaa
     6241 aagataagag cttctcaaca cttgtatatg catatgcact agcagcttca gagcaaaacg
     6301 cttctggtaa tataatagtt acagcaccaa cttgtggttc tgctggagta ataccaggaa
     6361 tattctttgc aatgcaggat ttctataatt atgatgacga aaaaataatt gaagcactac
     6421 ttgtagctgg aataataggt aatataataa aaactaacgc ttcaatatct ggagcagagg
     6481 tagggtgtca aggtgaagtt ggagccgctt gttctatggc agcagcagca gtagcatact
     6541 taaaaggtgg aactatagac catattgaat atgcggcaga aatagcactt gaacatcatt
     6601 taggaatgac ttgtgaccct gtatatggat atgttcaaat tccatgtata gaaagaaatg
     6661 caatggcagc tcaaagagct tatgatgcag ctaattatgc attattaact gatggctcac
     6721 attctgtctc cttagaccaa gttgttgaaa caatgaaaga aactggtata gatatgatgg
     6781 ataagtacaa agaaacagct aagggtggac ttgctaaaca tttcttctct tgctaaaaat
     6841 aaaattttcc aataataatt cctctgtttt gatttatgaa acaggggaat tattattaat
     6901 ggtgaattat ttaataatgc aaaatatttt tatattcaat ttagcttttt atttttaatt
     6961 agtataacat gctatataat attttaatta taaagaaata tagaaatatt ttttaataaa
     7021 ttagaaattg gaggagatta catgagagag gcatcatatt tacaagaaca actttataaa
     7081 cataggaagt cattaaatcc tattgcagag attgggagaa aagagtataa gacttcaaaa
     7141 tatataagag attatcttga taaattagga attgagtatg aagtttattt agaaacaggt
     7201 atagtaggtg taatagaagg taaaagacct aaaaaagata ttgcttttag agctgacata
     7261 gatggtcttc atagtgaaga aggggtaaaa catttatgtg gtcatgatgg acatgctact
     7321 atattacttg gattaattga gtatttaaat gataataaag aagacttaaa tgataatata
     7381 gtatttatat tccaaccagc agaagaaggt cctgggggag caaaagcttt aatagatgaa
     7441 ggagtgctta gaaaatacaa tattgatgaa atatatgggc ttcatatata tccagagtta
     7501 ccagaaggtt atgtaggtac aagagagggc tattttatgg ctcaaatagg agatattgac
     7561 atagaaatag tgtctaaaag tggacatggg gctatgccac aaaatggtat tgatggtata
     7621 gtgatagctt caaactttgt aagctcactt caaactatag tgtctagaaa tataagccca
     7681 atagacaatg cagtgttgac tattggtaga attgaagggg gagctagaag aaatataata
     7741 gctgaaaata taaagatgga aggtactatg agatgtttca gcccagaagt gtatgagaaa
     7801 atgaagctta gagttagaga acttgctaga gggtttgaat tggcttataa ctgtaaggtt
     7861 aatataaata ttatagatga ttatattgct gttaaaaatg acaaggattt gtatcaagag
     7921 tttgtagagg ctattggaag tgatactgtt gtagagttag agccacttat gatatcagaa
     7981 gatttttctt attatcaaaa agaagttcca ggattatttt ttatgttggg ttcaagaaac
     8041 gaagaaaaag gatttgtaaa tggtcttcat aatataaact ttaattttga tgaaaagata
     8101 tgtgtaaatg cattgaatgt ttattcaaaa ttattaaaat acaaagaagc aatagattag
     8161 gaagataagt tataaagatt aaaaagcata aatacctaaa tttaaaaaaa gtgtattaat
     8221 aaatacccct tacatatttg tataaattta ttttaattga caaaaataca aataagaagg
     8281 ggtgattttt ttgaaagaaa aagtacaaag agaaaagaag ataactaatg gcaagaatac
     8341 taaaatattg acaaatgatg atataatgaa atatgagata gcctcagaac taggtcttat
     8401 ggataaagtt ggtgaacgtg gatgggcagg acttacagct aaggaagcag gaagaattgg
     8461 aggtatgctt acatcacgca aaaagaggct taaaaagatt gtggaggagc agaataaaaa
     8521 ggatgagtca atatagtgat tttgcgtttg tatatgatga acttatgaat gaagttgatt
     8581 ataatggttg ggttaaatat atagaagata taatagatag tgaaggtgta aaagtaaaaa
     8641 atatattgga attggcatgt ggcacgggta atcttacaat tccattgact aaaaaaaatt
     8701 acgatatagc tggtatagat atatctgatg agatgcttag tgtagcaaga gaaaaggcag
     8761 aaaaagaagg agtagaatta gttttacttc aacaagatat atcagagctt gattttgaaa
     8821 tatctgattt ggactgtgta ctttgtgctt gtgatggatt taattatata acttatgatg
     8881 atgatttgga aaatgtattt tcaaagacat atgaattatt aaagaaagat ggaatattca
     8941 tatttgatat aagttctttt tataaattag ccaacatatt gggaaataac atgtatggag
     9001 aaaatagaga agatatagcg tatatgtggc aaaattactt tgatgaagaa gagaatctaa
     9061 ttgaaatgga acttacattc tttattaaag atgaatgtgg aaagtttgaa agatttgaag
     9121 agatacacca acaaagagca tatactgaag atgagatagt tgatatgctt gaaaaatctg
     9181 gatttaagga tataaaatat tatggggatt ttacatttga caaacctagt gaagaaagtc
     9241 aaagaatctt ctttgtatgt aaaaaataag atattcttga ctatataaga taattattaa
     9301 aaataaccat gatataaaaa tatataaaat gcagttttat agattaaaag taaagtgatg
     9361 atattttatg atttttattt tctaataatt ataaagtttt atagataaaa attaaaatat
     9421 aataactatt gcttagtaaa attaaaataa aatgggtttt gcccaaggtt aagatagata
     9481 agtatttata gattaaaatt aaggtagtaa tataataaaa ataagacaat taacgggaca
     9541 tggaggaata ttaatgagag attatgtact aagagcgaca tcaggaaatg gtcaagttag
     9601 agcatttgtc gcaactacta gaaatacagt agaagaagct agaagattac atgaaactac
     9661 aaaagtagca acagcagcac ttggaagaac attaacagcc acatctataa tgggattaat
     9721 gatgaaaaat gatagtgata aattgactgt aataataaaa ggtggaggtc caattggaac
     9781 tattatcgcc acatcagact ctaagggaat ggtaaaagga tatgtaggaa atccacaagt
     9841 agaggttgaa gactatccaa atggaaagtt aaatgtagca gcagcagtag gtacagaagg
     9901 tgttgtaaaa gttataaaag acttaggtct tagagaacca tataatggga cttatccatt
     9961 ggtaagtgga gaaattgcag aagactttac ttattatttt gctgtatcag agcagactcc
    10021 atcagtagta gctttaggtg ttcttacaaa agaagatgaa gtagagtttg ctggaggatt
    10081 tatagttcaa ttaatgccag atgcagaaga agagactatt gctaagttag aggaaaatgt
    10141 agctaaatta ccttctataa ctaacatgtt aaaagaagga aaatcaccag aggatatatt
    10201 aaatattgtt ttggatggtt tggaacctaa aatacttgat acctgtgaag ttgggtttat
    10261 gtgtgaatgt tcaaaagaaa gagtaaaaac tgctttggtt gcaattggta aaaaatcatt
    10321 ggctcaaata atagaagaag ataaaaaagc agaagttggt tgccagttct gtaataagaa
    10381 gtatatgtat agtgaagaag agttgcttga aattttaaag gaaatgtaga ctataaaaat
    10441 agttgtattg atataagtaa tgttatagta atatagtaaa gtcgtattga tatagtaatt
    10501 aaagtataat ttattaataa tagacatatt tatattaaaa aataagttta ataaattttt
    10561 aagcttaata aattttaaga attaagaagc tttctggctg taatgtttgc aatgttgcag
    10621 ttagaaggct tttttatttt attatagttc gtgtcaaaga ttgtatgaaa caataaaata
    10681 ttgtaaaata atttgtatat ttagacctga ttgacacaaa ggtttataag atatttgagt
    10741 tgattatacg aaattctatg aagtagtaac cctgtgactt taatcatggg aggttcagaa
    10801 gaaggagaaa attttaaata tggaaagtaa aacgaagtac ataggaataa gtatattaat
    10861 attattgatg atatttttaa ttgcaaagaa ttttaacgga aaacttgaaa tacctattcc
    10921 tgagagtaat agtagaaaat ttaaatcagc agcagtgttt tatcctcaac atcaagatga
    10981 tgaggtttta tggggaggaa gtgccattgt agatgctata aaacagtgtg gtgttgataa
    11041 tgtatatgtg gtattagtat cagatggttc tggagttaac gttttcaaag ctaacacaaa
    11101 atttagaaat ttaactagga agcaaaagga agaacttaga aataacgagt ttaaatcagc
    11161 tttgagagag cttggagtga aacctcaaaa tgtgattata cttgcagata tagataaaaa
    11221 ggaaggcaca cattatgaat taatggaaaa gactatattg gattttgaac ataagtttaa
    11281 aggcaatgtg acccatatag cccatcacta taagtatgat gaccatataa tgcatagaaa
    11341 gaatggggaa gttttaaaga aacttaaacg tgaaggtaag ataaaagatg acttgtattt
    11401 tgttaagcca aaatatgtaa agtatatacc aagaaatgaa agagctatct ataaagcaat
    11461 ggattttgat gactacaata aggttaagag tgcttgttat gaatataagg ttgtaaattc
    11521 aaagcgtaaa atgtatggta taggatatat ttcggcacat agttattttg aacatcttct
    11581 aaatgaccct gaacttacgt ctgtattaag tgaagattaa tttcttataa gtagtaagtt
    11641 atgtttgata agttgattgg aattttctat agttttgaag gtattggaaa tttagtttta
    11701 tttaaagatt taaagataga ggtttttagt gtttaaaatt ttaaagttaa gttgaataat
    11761 tgtatagaat tatttgtgaa agaagaaaca gtgtaaaaca tagtatctta tttatggttt
    11821 taaaatcata ggcaagatac tttttttatg taactaatac cattttgaga ttattaactt
    11881 aagaatttta tataatttaa cactcatata taatgatgat atttaggtag aatagatgta
    11941 attacgctta aataaaataa ctgtattttt tataaaaatc caaaccctac actatactac
    12001 cagataaaat ctacatgcta taatttgacc tgtgtttata aaaaaaggag taatctaata
    12061 aaatagtatt tcacttatgt ataactaaca actattatta cgattttctc taaataattt
    12121 aaagaaaaat aatgcaggag gtaaaatatg aacaatgaat ttataatttt aaaaggttgc
    12181 aaagagaaca atttgaacaa tatttcctta aaaataccaa aaagaaaaat aaccatattc
    12241 acaggggtgt cgggttctgg aaagtcatct attgtatttg agacaattgc aaaagaatca
    12301 caacgccaac taaatgaaag gtttagtaca tttgtaagaa gttttttacc taagtatgga
    12361 gaggttaaag ctgattgtat agaaaatttg tcaacaccaa ttatcataga ccaaagtaga
    12421 cttggtggaa attcaaggtc aactctaggt actattacag atgtaaattc ctttttaaga
    12481 gctttatatt caaggtttgg tagtgagtat ataggtaatg ccaatatgtt ttcttttaat
    12541 aatattgatg gtatgtgccc acactgtcat gggcttggaa aaaagctagt tcctaatatg
    12601 aaccaaattc ttgatatgaa taaatcatta aatgaaggtg ctatattgct ttcagggttt
    12661 ggagtgggtt cttggcattg gaatatattc gctgaatcag gattttttga tattaataag
    12721 aggatatgtg attattcaga agtagaattg gaaaagtttt tgcatggtaa agcagaaaag
    12781 attaagatag aaaatgctgg tcaaactaac atgacatatg agggattaat ggttaaattt
    12841 aatcgcctat acttaagtag agagggagaa atatctgaag ctactaagaa aaaattgagt
    12901 aaacttctta tagaagatag atgtcctata tgtgatggaa gaagacttaa tgaaagagta
    12961 tataggtctt taattaatgg ttataatata gcagatttaa ctagtatgca aattgatgaa
    13021 ttggctgaag ttattaaaag tatagatgag ccagaagcac aacctttaat taaaggaata
    13081 atagaaaagt taaacagtat catagatata ggtcttggat atttaacact agatagagag
    13141 acatcttctc tatcaggagg ggaatctcag cgtataaaga tggttaagta tttaaatagt
    13201 aatcttgttg atttaatgta tatttttgat gaaccaagtg taggacttca tccaaaagat
    13261 gtgtataaat taaataatct tttaaaaaaa ttgcgtgata aagggaatac aattatagtt
    13321 gttgaacatg acccagatgt tattaagatt gcagaccata ttgtagatgt aggaccaaag
    13381 gcaggtaagt atggtggaga agttgtgtat gaaggcagtt atgaaaatct acttacaagt
    13441 ggtactttga cagggaatgc tttaagtaaa tttcttccta ttaaagaaag tgtaagagaa
    13501 catagtggat atttagaagt taaaaattgt aataaaaaca atttaaaaaa tgtatctatt
    13561 aagataccta aaggtgtatt gactttagtt actggagtag caggagcagg taaaagtaca
    13621 ttgattaagg atgaattttt aaagcaaaat cctagtgctg tacttataga ccaaagccct
    13681 gtttcagcaa attcaaggtc gagtttggca acatatagtg gaattatgaa caacataaga
    13741 agcatatttt caaaaacaaa tggcgtaaat gcttctttat tcagttcaaa ttcagaagga
    13801 gcttgtgata actgtaaggg ttctggaata gtagaaatga atcttgcttt tatggagagt
    13861 ataaagagta cctgcaatat ttgtgaaggt aaaaaattta aaaaagaagt tttagaatat
    13921 aggtttcaag ggaagaatat aattgaagtt cttgaaatgt ctgttttaga ggcaattgag
    13981 ttttttaatt taaaacagat taaaacgaaa ttacaatgta ttgaaaaaat gggtattgga
    14041 taccttactt taggacaaac tcttgatact ttgtctggag gagagtgtca aagattaaag
    14101 cttgctagtg aattaaataa ggaaagttca gtgtatatat tagatgaacc aacaacaggt
    14161 cttcatatgg ctgatattga gaattttata agtataattg aagagattgt agataatgga
    14221 aatacggtca taataattga acacaatata gatgtaatta aaagagctga ttggattatt
    14281 gagctgggac ctgaaggagg aacaaaaggc gggagagtaa tatttgaagg tattccaaaa
    14341 caattatgta acaataaaat atcattaaca gcaaaatata tagtataata aataaaatat
    14401 aataaaatta taatgatatt ttaataaata taatgggtat ataaaatgat aattaaactt
    14461 aacagtaata aataatgtga gaaatattca aatttaatta aataaagtgt cagaaatttg
    14521 tttaaatttt agtattttaa cggttctgac cttttttctt atttttatgg agaatatcga
    14581 ggatggagtc tctaatagtt acttttttgt gtctacttgc aagtttgatt aaaaaatggc
    14641 tatacacact tgtgacttca gtcatgggag gttcagatta taatcttaat atataatttg
    14701 tgaaataatt tgatgacgat ttgctgatgt ataaacaata aagattaact acattaaaat
    14761 ttaaatcttg ttaagtaaaa cttataaaag gagagagatg gatgataaat gatattctaa
    14821 ccttattatc tgaaagaagt gattttttta taaagctatc catagaacat ttgatgattt
    14881 ctattatttc tataataatt gcgattgttt tgggtggtat aattggtatt attgtaagtg
    14941 aatttcataa ttcttctaaa cctatattgg gaactatcaa ttttctatac accattccat
    15001 ctatttctat gctaggattt ctgataccat tttccggaat tggaaatact actgctgtta
    15061 ttgcacttac catttatgca ttattgccaa tggttagaaa tacatatact ggaattacta
    15121 atgtggataa aaatattctt gaagcagcta ggggaatggg aagtacagat tttcagatat
    15181 tgtataaaat aaaacttccg ttagccctac ctgttattat gtctggtatt agaaatatgg
    15241 tgattatgac aattgcatta gctggtatcg cttcatttat aggtgctgga ggcctaggtg
    15301 tagccatata tagaggaatc acgacaaata acacaataat gacaattact ggaagtatac
    15361 ttattgctct attagcttta atatttgatt ttattttagg gctggtagaa aaattttttc
    15421 aacgaaaaag aaaaaagaaa aataggaaaa aagtttttgc cattctagga agccttattc
    15481 ttatagtaat tatctttacg tcttatttat ttaatccttc aaagactcaa actattcata
    15541 ttgctactaa acctatgact gaacaatata ttcttggtga gatgttggat attttaattg
    15601 aaaaaaacac aggtctaaac gtggaaatta cacaaggtgt aggaggggga acatctaata
    15661 tccagccagc tatggagaat ggagaatttg acatttatcc agagtataca ggtacaggct
    15721 ggaatatggt acttaagaaa aatgaattat atactgaaag tatgtttagt aaactacagt
    15781 cggaatacaa aactaaactt aaaatgcaat ggactggtat gtatggtttt tctaatagct
    15841 atgcattagc agttagaaaa gaagttgcag ataaatacaa tttaaaaaat ataacggact
    15901 taaaagatgt atcttctaaa ttgacatttg gagctgaata tgacttttat gagagagagg
    15961 atggatatac tccattgtgt aatacatatg gactgaactt taaagataca gttgatttag
    16021 atattggatt aaaataccaa gctatcaatg aaggtaagat tgacgtaatg cctatattta
    16081 caactgatgg tcagtatagt gtttcagatg ttgtagttct aaaagatgat aaaaatttct
    16141 acccatctta tcaatgtgga aatgttgttc gtttggaagt attagaggaa aatccagaac
    16201 tatcaaaggt atttaagatg gttggagata ttttgactga taaagaaatg gctaaaatga
    16261 attatgacgt agaaaacaac aatttagaac ctagagaagt tgctcgtgag tttctgaata
    16321 agaaaggtct tttataaatt ggaggaaata taatgacacc tattatacaa tttaaaaata
    16381 ttaaaaaaca atataatgat aaaactataa tagataatct taatttagat atagaaaaag
    16441 gagagttttt gactgtaatt ggttcgtctg gttctggtaa gacaacactc ttaaaaatga
    16501 ttaatggtct gattttgcca gatggaggaa atattttgat aaaccaaaca gatatcaaaa
    16561 acgaggactt aattaaatta agaagaagaa taggatattg tgtacaggga agtgttcttt
    16621 ttcctcatat gactgttgaa gaaaatattt catatgtacc aaatctccta agtaaaaaga
    16681 ataaattaga agtaaaaagt gctgtaaaca agtggatgga aatagtggga ttgccaaatg
    16741 atatgaaagt gcgttaccca tcagaattgt caggtggtca gcaacaacgt gtagggattg
    16801 caagagcttt agcttcttct ccagaaattt tattaatgga tgaacctttt ggtgcagttg
    16861 atgagattac acgaaagcaa ttacaaaaag agataaaaga aattcataaa aaaacaggag
    16921 ttacgattat ctttattacg catgatattt atgaagcttt gattttggga acaaagactt
    16981 tggtattaaa tcatggagtt gttcaacagt atgatactcc tgagaatatt ttaaatactc
    17041 ctgctaacca atttgtagac cagttattga atataagaaa atctataata gatgatgata
    17101 atttaaaaga ataaatttaa atttaaaatc aaattgttgt gataaaaact attgatttag
    17161 agctaacttc aagttgtata cttgaagtgg aaaagttaga ttaaggtata gaagggcatt
    17221 tagcagttgg ctgtagacga ggtaactgct gaaggtgctt tcaataaatg gctagaacct
    17281 gtatctgttg aagattataa taaattgaaa taagaagctc aatatacaga gtagataggc
    17341 acttcaaata gaagtgtctt ttgtgtgcca taaaataaat caaaatatta gcaaagaatg
    17401 tgaaaaacaa aataaaataa tatgctatct ttaaaatagg agggatgcta tatggataac
    17461 aatttgatta ccattgaaaa aactttagat tatatagaaa aacatctttt agatgagatg
    17521 ttagatttaa atgtaatttc taaagagata ggctattcaa aataccattt acatagaatg
    17581 tttacatcag ttgtcggatt tagtgtacat aattatatac aaagacgtag acttactgaa
    17641 gcagcgagaa tgctaatttt tacagaaaaa ccaattattg agattgcttt aatctcgggg
    17701 tatgatacac aacgctcttt ttcaagaggg tttaaaaaaa tgtttaaacg tagtcctaat
    17761 ttttttagaa gacatcaaga ttttttacca ctacaattaa aatttgatat tcataataga
    17821 aaaaaattac gtggagatat ggttttagat gttaaaaaag ttaattgtgc agatataaat
    17881 attgttggct attctggtaa agtaagaaaa ggatttcata ttattggaaa atgctggagg
    17941 gatttgcata aaaataaaga gatgatagaa aataaggtag atttaggttt tttaattgga
    18001 gttagtgatt atagcaattt taaagagatt gagaatactc ctacatttac atacattgca
    18061 ggtgctcaag tagctacttt tggcaaatta cctaatggaa tggaaaaatt cgtattaccg
    18121 aaaagtaatt atattgtttt tacttttatt gggaaaaatg aggatagtat gcaaaatgtt
    18181 gtagaatata tctatcaaga atggttccct aattcaagtt gccagtttaa tgaaaataat
    18241 ttatatgact ttgtaaaata tggagaaaaa actgatgaga atggtttgag tgaaattcag
    18301 ttttgggttc caatcattta aaagaaggta ttatgtataa agtagatgga tttatatagg
    18361 caaagtttta cagcagtatg tatttataaa taatttatag tttgtaggaa agaaataatt
    18421 ttactataag attatgataa aatacacaat actatgacaa aatagtgtta aataatagat
    18481 ttaattttgc ataggaaaag ttctataatg gtgtgtattc ataaataatt tataatctgt
    18541 aggaaagaaa tagttttact ataagattat gataaaatac acaatactat gataaaatag
    18601 tactaaataa tagatttaat tttgcaaagg ataaggtgtt ttaataaaaa tgaatgtaga
    18661 aaacgaatta cttaaaaacc tagatagatt gcatacaaca gagttaggcg ttgtaagaat
    18721 taagaaaaat ctttctttgg agacaaatga tgtagtgaac tggtgtaaaa ctaaaataga
    18781 atctccaaat gccattatta atagaaaggg aaaaaactgg tatataagtg tatatgattg
    18841 tattataaca gtaaacgctc acagttatac aattattaca gctcataaag aaaagaaatg
    18901 aattgcaaaa tacaaaggtc aaaagcattt tccactgttt gtagtaccta tgaagaagta
    18961 tataaaataa tattactttt gaataataat ttaaattttg gaggttgaaa aaatgaatga
    19021 tactgtaaaa gaaaaattgg cagagtcact aacttctgat actactgata ttttacctta
    19081 tttgccttat ttattacaag atttttggga attaggaagt tcaccagaac aaatgttaca
    19141 attatttaga aagcatatac cttttaatga ggatacaaaa gttttggatt tagcttgtgg
    19201 aaaaggtgct gtatctgtga agcttgcaaa agagttgaat atacatatca aaggtattga
    19261 tttgatacct gaatttatta attatgctag aagaaaggct attgaatata aggttgatgc
    19321 tttgtgtgat tttgaaatga atgatgctaa tagttctgta aaaattgagc atgattatga
    19381 ttgtgtgatt tttggagctg taggggatat tttaggtaat tataaagaaa ctataagtaa
    19441 gctattacct acaataaaaa gtaaggggtt cattttaatt gatgatgcat atgtagtaga
    19501 taaatctaca aatgaacagc ttaattttga gagggtgtat cccacttaca atgagtggtg
    19561 tatattgttc aaagaattag gtctgaagct aattgaatgt ataccatgtt tagaacttga
    19621 aaatgatgtt gaagaggaaa tgaattggat tataactcgt gcaaatgagc taattaaaaa
    19681 atatccttct caaaaagata tgtttgaaca atatgttatt agccaaaaaa atgaatatac
    19741 tgatttacag caggaccttg ttggagcaac ttggatttta caaaaagaat aagataaata
    19801 ttagactaga taatcaatgt tatagtgaat tatcccatgt ctagtgaata taaataacat
    19861 aaaaaaccgt caagatgaat agtttattcg tgcttgacgg tttttagtat ttgttgaagt
    19921 aaaattttat ttctttagta tatcactttt agtcaatcca tttgatttag cttgagtatt
    19981 ccattgatga ttccccataa gtttatatag gaatctaggg aagttaggtg aaataaaaat
    20041 agtacttttg ttggaatcat ctatatgtat attatcacta agtatatttt ttgaaagttc
    20101 ttttaatgca attcctaaat cttttttagg tcctttaccc aaaggtacgg attctagtga
    20161 accaaacatc tccccagctc caattccaat accttgaacc catgtaaagt tgattttttt
    20221 acaccaatgc tctatcattt gaattgccaa atgattatgt tcaccttcat tgaatccaca
    20281 gttaactatt gcatacactg gaataggttt tttattttgt gtttttaaaa atttttcaca
    20341 tttctctaaa aaatgaagta aatgagatgg aataccatct acataaagag gagatgcgat
    20401 tattaatcta tcacaattaa gtatggcatt aagagcgtca ggatacttag atgtgctatc
    20461 tactttaaat gtaaaaactt tattatcatt ttgaataagt tgttttaatt cagataaaaa
    20521 atactgtgaa ttatttttat ttacttttgg actaccattt atcattacta tattcatatg
    20581 gattcacctc ttatatcttg tatattttca tagaaacaaa cagagtgttc ttttatattg
    20641 aaatttatag catttccatt tactaatttc gtagcagttt ctttttcagc ttctgtaata
    20701 caatctccat agaatatagc ttttaaattt atctgtttat catatctgct tttatgacgc
    20761 atcatattat cttttattac aaagtatgga agaatataat taatgctttt atcaagtacg
    20821 tttttgacaa aagggctaaa ccctccgtaa cagcatctgc ttattataat aattccatca
    20881 gagtcattta taagtttacc catgttgctg taataatctt ttacaacaca ttcagaagga
    20941 gttttaatcc agcaaccaaa acaaccaata catttgtgga tttcttttgt gtttggaagt
    21001 atttttatat tgtcatcaag tttgggtaat aacttttcta attctttgtc agttaaatca
    21061 tgtattaaaa gcctcataaa gtccatctcc ttttattatg tatgtttact tctaaaaatt
    21121 atatcttata gatttttcag taaatcttta caccaattaa taacaaaatt ataatagtca
    21181 actccataaa tcattgttgc atacatatat gtatattggt caacgcaatg attttcttct
    21241 gcatcatttt tttgtttact taatttattt agaagtaatt ctacttcagt tattaacatt
    21301 tttaagtttg tttcaattat ttctttgggt agatacttgt aaaagaatat attaacaagg
    21361 tggtcttgct ttgttttaga aaaatttata ggtttcttta accattcaag aaatacttct
    21421 ttaccagtat tagttatgga atataatttt tttaatttgc taccatctat aacttcgtga
    21481 tagtgtatat atccttttgt ttctaatctt ttaagtgcag gatagatact tccaaagctg
    21541 gcgtcaaaaa agtacgaggt actttctgac attatttgtt ttaagtcgta tccacttaat
    21601 tctttttcca ttaaaaatcc taaaataatg tattctaaca tattacctcc tggtatcaat
    21661 tagatatata tcgattcgat atatttatta ttacagataa taaatcttac gtcaagatgc
    21721 tttttaagat aagctaaaaa gaattaggag ttattgataa atatgcaaaa aaattaatat
    21781 aaaaggtaat taaatatcaa tgaagattta aaaatagtta aatcaaagta agagtctaat
    21841 atataaaatt tatagtaact ttatggttta aatataaact tgcgtgctat tgacaaagta
    21901 taaaattagc agtaaaataa ttgcatgagc aaatagatta ttgcgaacgc aaaaaaataa
    21961 ggacaataga tattaaagga gagaatatga gagagctatt aaaatgggtt tccattataa
    22021 atagatacaa tacaaattat attgacagga aattggcgga tattggaatc aataatagcc
    22081 aatattttta tgtattacac atatgtaata atccaggtat tacacaggac actatatttg
    22141 agaaaattct tgtgaatcct agtaatataa caagagcttt ggcaaattta gaaaaagaag
    22201 gctttataac aagagaacca ggtgtaaagg ataaaagaac atggcattta tatcctacag
    22261 aaaaatcttt aggatgttat gatgaaatac taaaaataac aaatgggtat gttgaggagt
    22321 tgttgagttc atttgagaaa gaagagcaag agttgtttat gtcaatgtta aagaaagttg
    22381 catttttggc tattgatatg aatgaagaag caaaattgga ggcgaaggaa aatggaagaa
    22441 aaatataagg agccattgga atatgaaaag gtttcaaagt tattaactaa gtacgcaatt
    22501 cctagtatta tagcaatgtt agtaagtgct ttatataaca ttgttgacca aatatttatt
    22561 gggcaaggtg ttggtgtatt gggtaatgct gctacaaatg tcacttttcc actaacaaca
    22621 atatgtactg caattgcatt attgttagga attggaggag cgtctaagtg ttcattggaa
    22681 ttaggtgcta aaaattcaga aaaagctaca aaagcagcag gaaattctat atgcttgatg
    22741 gctgtttttg gaataagttt atttatagta gtatcaatat ttttaacacc actattaaaa
    22801 ttctttggct ctacatcaga aatactacct tatgcacaca catatacaaa aattacatca
    22861 attggattac catttcttat aatgtccact ggaatgagta agttgatact tgcagatggg
    22921 agaccaaaag cttctatgtt ttgtatgtta ataggagcag ttattaatac aattttgaat
    22981 ccattattca tttttgtttt taatatggga attgctgggt ctgctttagc aactgttatt
    23041 gggcaactta tatcttttgg aataagtgtt cattatataa agaattttca acatattaaa
    23101 ttacataaga aaagttttaa attagatagt ataatttata aacatatatt tgcgctaggt
    23161 tcaagctcat gttttaatca attagcaatg actgtaacac agatagttat gaataataca
    23221 ttggcatatt atggggctag gtcagtttat ggaagtgaga ttccacttgc ttgtgtcggt
    23281 atcattatta aagtaaatat gatttttata tctataatta ttggtatatc acaaggtatg
    23341 caaccaatta tagggtataa ttatggggct gaaaagtata atcgtgtaaa agaagcatat
    23401 aagtttgcag tgggaagtgc aactattata tctgttattg cattcttatg tttccaattc
    23461 ttcccaagac aaattacgtc tatatttggt aacggaaatg agacttattt tatatttgca
    23521 gaacattatt ttagaacttt cttgttctta acatttataa atggtgttca aatagtatca
    23581 tcaactttct ttacatcaat tgggaaatca gtgttgggac tttttacttc tttgactcgt
    23641 caaattttat ttttaattcc attgatagta attttaccaa tgtttatggg aattgatgga
    23701 gtaatgtttg caggaccaat agcagatgca gcagcggcaa tggtatgtat atactttgca
    23761 gttcatgaaa tgaaatcatt gactataaag caaaataatt tgatgagtaa ccataataag
    23821 attgcagagg tataagacaa tatttaagaa gtatagaaaa ttaatttaaa aaatataata
    23881 aattaattta ggaagtgtag aaaattaatc taaagaagta taataaacta atttaggaag
    23941 tatagaaaat taatctaaag aagtgtaata aactaattta ggaagtatag aaaattaatt
    24001 taaagaagta taataaacta atttaagaaa tatagaaaat taatttaaag aaatacaata
    24061 cattaatttt taagtaacat taaattaatg taagtaaaat aatgttcata tgaatattta
    24121 aaggtgcatt ttctgaagct aagtatatgt tgtagagtgt tatgtctctc tatcctgtga
    24181 agtttaatag ctcaagaaaa tgcactattt atgatgatta ttttgtttta tttccaattt
    24241 tggaaataac tattctaatt aaatctaaaa ttataccata aataagaaaa gaaagtatag
    24301 atgctatgta ccaataaatc gaaattgacc ctgtaattgt tctacatggg atttgcatac
    24361 ctgaatccat aaaaacaaaa aaactatcaa ctctggaaat aatagacaaa ataggattta
    24421 aacctattag aacaatactt tttgagtctt gaccaatttg atgcatataa atctcaaata
    24481 atgcaattaa aaaaaaccaa gatgtaaaag atttaataat ttttttcatc aaaatacctc
    24541 acataatata aattctgttt gcactatttg cttactacaa ctatttatct ccattcagtc
    24601 acatcactta attgtaatct aggtcttctt gatggttctt catcaggata acctaaagat
    24661 attgctgcca ctaattgact atctgtattt aaccattcac ataattcacg atatgcaaag
    24721 tatacatcac aaatccaaag actacctaga cctaattcca cagctgtaag cgacatattc
    24781 tgaattgcag ctccaattga ttgcatattt gccatttcat agaatcgttc ttcaggggat
    24841 agcttttcta aaggtgattt acctaatata tttaaaataa aaattgttac tggagcttgt
    24901 ttcatgattt caactgtgta atttgcacca gcgatatgtt gacgacttcc tggaagaagc
    24961 ccattatcat taatttcatt ttgaatacct ttgctcatgg ctttaagcat agattctttt
    25021 tccttttcag tgatgacaac aaaacgccat ggttgcctgt ttttagatga aggtgcattt
    25081 attccagctt caattatttt ctctatagtt tcatgtgaaa tgctttgatt tttatatttt
    25141 ctgatacttc gtctttttga tatagaatca cttatcattt taagtaacct ccgtttattt
    25201 tttggaaaaa tctatttaat ttttattttc tattttaatt attttagcat taatcagaaa
    25261 aaaagtcatt gattttagta tacaattatt aatgattata ttaagaattg tatcaaaaag
    25321 tatgtgatta tactaaggga ttatattaaa cattatattt aaaattgtat gaaaggttgt
    25381 atttaat
//
LOCUS       DAMJYR010000048        25214 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.048, whole genome shotgun sequence.
ACCESSION   DAMJYR010000048 DAMJYR010000000
VERSION     DAMJYR010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 25214)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25214)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25214
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.048"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            257..931
                     /locus_tag="PC471_001922"
     CDS             257..931
                     /locus_tag="PC471_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2789052.1"
                     /translation="MNNKILVVDDEEGIITLLKDYFEMNGYEVYTAQSGKDALKKISK
                     NPDIILLDINMPEIDGIEVCNRIRDYVSCPILFLTARIENTDKINGFRAGADDYIVKP
                     FDLDELGARVSAHLRREQRKSNQTKIKFYNDLTIDYEKRLICIYNNQISLSKKEFDIV
                     ELLSMNPGQVFDKEKIYDRVWGYNSNGNSDVIMEHIRKIRIKLSKHTLENYIETVWGV
                     GYKWIK"
     gene            919..2079
                     /locus_tag="PC471_001923"
     CDS             919..2079
                     /locus_tag="PC471_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HDF2789053.1"
                     /translation="MDKINNMSLKKAFFVLTLSSLIIASVLTTFAYILLNNLYNSIQD
                     KYLKTPINTGIITIVQNNVNQFSDYDKTLLNIINILQLVLPLIFFIGLLLLADIIFYK
                     VKLKTPIEILNKGAMEISNNNLDFCLEYNNNDELGNLCNAFEKMRSELNKNNIKMWTM
                     INDRKQLNAAFSHDLRNPLTVLKGYSNYLTKYIPTGKLSDEKILSTTQLMSEHINRIE
                     YYVENMSNAQRLEDLVVSKSMSNINKFIENLDENISIIAKQEGKSFTLKSQINNINLF
                     FDENIILRVVENIISNAFRYARNNVSILIYLEQELLTFVIEDDGIGFSKESLKLALRP
                     FYRDKTLNDSNAHFGMGLYISKILCEKHGGSISIENNSTNSAKITAKFSTRN"
     gene            2295..2822
                     /locus_tag="PC471_001924"
     CDS             2295..2822
                     /locus_tag="PC471_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014118918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HDF2789054.1"
                     /translation="MKSFANNITFSRIVFALLMLSSTFSSLPFWICYFWCGISDILDG
                     LIARKLRQQSGIGAKLDSIADFIFAVTILVTVGKNIALPMYLWWCIVAIALLRLISYS
                     IGFFKYRTFSSLHTLMNKITGISIFAFPLLYICFGLDVAGIMICIVAFLSSFEEIAIM
                     IKSKELNRDIKSIFM"
     gene            3108..3776
                     /locus_tag="PC471_001925"
     CDS             3108..3776
                     /locus_tag="PC471_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2789055.1"
                     /translation="MIIKAKQLSKIYGSNNNKVIALNNVNLEINSGEFVSVIGPSGSG
                     KSTLLHILSGLDNPTSGQVLLDDKDIYKHTEKELSALRRKSFGFVFQQFNLLPVLTAS
                     ENISMPVLLDKKQPDKDYLNEISSLLGIADRLNHLPHELSGGQQQRVAIARALIAKPD
                     IIFADEPTGNLDSKSGSEVMNLLIKTSKQFGKTLVVITHDDRIAKLADRKISIIDGVL
                     MEVK"
     gene            3779..6247
                     /locus_tag="PC471_001926"
     CDS             3779..6247
                     /locus_tag="PC471_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2789056.1"
                     /translation="MKNYLSLSIKELKSQKFMTTFIIIAIVLSTIMTTVVGQSIGILQ
                     NLRIEQARSFNGDRHVSFHQLTKNQVDDIKKDDRVYQAGTSITIGSSKIKDSGISVLV
                     KEYDKTGLSNYPKLMKLKSGHLPKDKNEIALDENTLKLMGIKPRLGVTIPMNLDISLL
                     NDTIPPYNYTANFKLSGILEDDYTGYVSGIVNGIVGKGTSENLLPKRYILSSLDFKIK
                     QQEKFQEIVNQLAKKINLSHNSIQYNWIYLNALKIQFEKDENSSNSDGISMIILVSLF
                     VALLVLLASGLVIYNILKISVTKKIKEYGCLRAIGAEPNQIYKIVILQILILCTVAIP
                     IGAVIGIISSKGITGMVTNILNPDILLANDNKEITELIHKNTTAYIFPLVLSTNVSLI
                     FSFISALPSAIYASHVSPKIAMVGSTTKIKRKIKREKTIKNFERHLAWLNLKRNKGRT
                     IITILSLFMSITVFVALSEFSNVLDVSRSVSNLKEGDFSLTNEISGFDKSTLDKIAKM
                     KNVNRTSFIKYSEYKQGEIDTDINFENSGEMLKIIGIDEQTLKDLMPSITDSILEDFK
                     NGSICFIKNPIAISTPGVKTRYTNLKPKDSITINKKQLDIYSTVDKMFFLQGNGWING
                     VDVIVYDSVYNTLTNKNKFNQINIYAKDKSKLEQIRLSIEQICENNPGSHWISYIESD
                     KQLKESFKQIEFLAWAVILFVGLIGTLNIINTTHTNINTRTNEIGVKRAIGMSNRSLY
                     KMFLWEGVYYGIFAAIFGSIAGYASAIIINMATIEKLDFTNIPITSILQATIISVLAC
                     IIATLIPLRKVKKMNIIDCIDINL"
     gene            complement(6487..7911)
                     /locus_tag="PC471_001927"
     CDS             complement(6487..7911)
                     /locus_tag="PC471_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF2789057.1"
                     /translation="MKNQSLITQFRHTFIFIIIASIVATVITYVFALYLYIHSLNKDI
                     YPPNYYERQVPRIEKYINEKNIALLSQSNEEGLKRTIRGDDMLYQVVDNNGNILYGTN
                     PKKLFKTKEELFNNFINKTVRKGGYIHTVPIKGDNGKIEGAVILFYQVKITFANIRGR
                     FVFAVIIMALFSPFLYIVGFTRWLSKRFVKNINQPLHLLIDASKKIKEKDLDFEIDYY
                     SDNELGKLCSAFSEMKDELKGSLSAQWKMEQERVEMVEALAHDLKSPLSIILGYTDAL
                     IGNNTDDNEKLHRYLTVIRENTEKSAALVQKMQYTSDLEKSNIQLNLVPINLPEFLRQ
                     KVQDYELQAHQKEVELILKMQGNIQSPIQIDVDRLTRIFDNIISNSLQYTPSGGNISI
                     TVKDEKNCISYEICDSGRGFSSKDLKKALDKFYRGDEARQTKGGHSGLGLYIVKQLVE
                     QLGGSVKIENSKSGGACVKFWHSI"
     gene            complement(7930..8622)
                     /locus_tag="PC471_001928"
     CDS             complement(7930..8622)
                     /locus_tag="PC471_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2789058.1"
                     /translation="MSKILIIDDEMDLVMLLEDELKAKGHEVLIAYDGQAGIELSKQE
                     PDLIILDIMMPKMNGFEVCQAIRDDVLCPIIFLSAKQSETDKIKGLTFGGDDYVTKPF
                     GLRELMARIEANLRREKRSQYINEENKRSTLYFGKLCLYMKERAIKIDGKNIDLTKIE
                     YDIVELLALHAGQVFSREQIYEKVWGCDSEGDSSTVVERVKKIRAKFSAVTPEKEYIS
                     TVWGIGYKWNKM"
     gene            complement(8674..9453)
                     /locus_tag="PC471_001929"
     CDS             complement(8674..9453)
                     /locus_tag="PC471_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic immunity ABC transporter MutG family
                     permease subunit"
                     /protein_id="HDF2789059.1"
                     /translation="MIKLLSSEWLRAKRTAVQWLTLCMPIIFSLCVVFYLTTKSGSTQ
                     EFAFEGFFTVWTVFIIPIGVGILAGFIVQEEELAGNFNGFLCVGISRVRLYLGKFLFL
                     LFCLTICTFIATLILCIGMSIAVPSGATIWLFLSAAGLVVIGTLPLLAIHLWVSFAWG
                     MGASIGISIGGILMAAILGLTSVGEKVWAFVPWTWPVKLGMLPGIYFIKEAGTISTEA
                     FYSKVMQTASIELIVVAIGLIIFLIGGVIWFKMWEGRKSYE"
     gene            complement(9450..10220)
                     /locus_tag="PC471_001930"
     CDS             complement(9450..10220)
                     /locus_tag="PC471_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic immunity ABC transporter MutE/EpiE
                     family permease subunit"
                     /protein_id="HDF2789060.1"
                     /translation="MNALQSELLKYKRTFMGKLIVFFPVSFAAYAFIMQSTLMQNPLS
                     QTTSWAWQNLLALIFNWWSFLFLPIGFALFATLVAFQEKKAGNYRALRTHNVSPMTLW
                     INKVIAMAVYSFISTLVLIVVTIITGLILKAGPVPFGQIIGASIVCWVVSLAILPLQL
                     WLATWKGMFLSMGVGALGMIFGVLAATKPFWIAVPWSWAVRMVCPIINIHPNGTILEA
                     GDPLLNTSVIPIGIVVSLVVFIVLTALTAAWFNRRDDK"
     gene            complement(10217..10921)
                     /locus_tag="PC471_001931"
     CDS             complement(10217..10921)
                     /locus_tag="PC471_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014466355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic protection ABC transporter
                     ATP-binding protein"
                     /protein_id="HDF2789061.1"
                     /translation="MKDLILETKNLSKRYGEQMAVNNVSLQIERNSIYGLLGPNGAGK
                     STTLKMLVGLLRPTNGRIIFDRKPWKRESLAKIGSLIESPALYGNLTAEENLLVHTKI
                     LGIPKDKIYEVLEIVNLKNTGKKRASQFSMGMKQRLGIAIALLSDPELLILDEPTNGL
                     DPFGIQELRELIASFPKKGITVILSSHILSEVAQVVDNIGIINGGRLLFQGMPDPNEN
                     LEEFFTDVILKGGQNR"
     gene            complement(11176..11688)
                     /locus_tag="PC471_001932"
     CDS             complement(11176..11688)
                     /locus_tag="PC471_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2789062.1"
                     /translation="MKKISQEKISTHIQEIPQEKIEQWKSLYEKYKSILKVNSKSVLE
                     IIEFLKRKYVIVEETSEEFKHVVINNIKLNKPFSQKISSDKELKPIVFTIPNEGNAKK
                     LYDTQEEIYSDCKIIVGMEFETGCVFVEGSDELNDEITSFKGLDKYDINNYYLVANYI
                     RCLKKYKLID"
     gene            12014..12271
                     /locus_tag="PC471_001933"
     CDS             12014..12271
                     /locus_tag="PC471_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2789063.1"
                     /translation="MILNRAELFRGYSLNRFNEICNILSVNKIKYKYKVKNNAYSKKP
                     FFNEITLGNLGQKEDFSYEYLIYVHKDDYEQAESLINSKLR"
     gene            12414..13040
                     /locus_tag="PC471_001934"
     CDS             12414..13040
                     /locus_tag="PC471_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HDF2789064.1"
                     /translation="MKINIKSKLSEFIKQNNLFDDSRIISYLPNITIETDKIKTIMIN
                     EVVPTNTNDDFYSVDKDADYLKTTIPLFDSAGIKVSNINDILDMGIYITNAVKLPKSE
                     YTITRDTIKLHMPILEEEIKLFKNLEVVMLMGDVAKKSFNMITKKHIKKNVIPSISTY
                     KLRNNELYYDNLRVFPSYIMTGGNILIEKSKFEMASEDIKKMFEIING"
     gene            complement(13287..14066)
                     /locus_tag="PC471_001935"
     CDS             complement(13287..14066)
                     /locus_tag="PC471_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901940.1"
                     /note="N-terminal region resembles flavodoxins. C-terminal
                     ferrodoxin region binds two 4Fe-4S clusters; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EFR1 family ferrodoxin"
                     /protein_id="HDF2789065.1"
                     /translation="MNSKIYYFSGTGNSLVVSNILKETLNGSKEVIPLAIHRKENNID
                     INEDILVIVFPVYFADVPDIVKSFVEKLNFNINTHIYAIATCNGVAGHSLFTIDKLLK
                     KRGKKLSAGFLINMPGNALITPPDVEEERLKNQTNRVAEIAKCINNLEIGNIEGKNNL
                     KSHIDSVVLRLVGKNMQIATTKFFAENKCNGCGTCKRVCPVENISIVDKKPKWGNECE
                     RCLACFHWCPKEAINVKKSFLTNRKKYHHPDITVKDIFLDK"
     gene            complement(14134..14595)
                     /locus_tag="PC471_001936"
     CDS             complement(14134..14595)
                     /locus_tag="PC471_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDF2789066.1"
                     /translation="MGGARDYLNGGKMKDFEKLYEATTKLVSTSLKVKDDLKKMFKQN
                     GYDITTDHYALLRFLWEQDGISQIDLCEKSCKDKSNTTRILDVMKNKGLIVRKVDVKD
                     RRKFQIFLTDLGRELEEPLNEIASIYATETFKSISDEELPLFTDILDRIGK"
     gene            complement(14571..15248)
                     /locus_tag="PC471_001937"
     CDS             complement(14571..15248)
                     /locus_tag="PC471_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HDF2789067.1"
                     /translation="MIITVINGSPRKNGATSKVLTYLYKDIERLIPDVKINYFDLSEV
                     NPSYCIGCLNCYKMGKCINQNDKVEYIHDIITKSDGVIFGSPTYGSSVTGLFKVFTDR
                     AHMMLERLLYRKPCIAVTTYENARGSKAISFIKSMVLDSGGYVCGSLSIKTGFNQNPI
                     TEKVESKIQKVSKKFIYCIEEKKNPPVLSQIYNFIAINAVLKPMAFKDIEQYKGIIDR
                     WEEQGII"
     gene            15481..15867
                     /locus_tag="PC471_001938"
     CDS             15481..15867
                     /locus_tag="PC471_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HDF2789068.1"
                     /translation="MKKLPHISEAEYQVMKIIWKYAPISTTEVIEKLVETSTWSPKTI
                     QTMLLRLVKKGALTYEKNSRVFVYTPLVKEEEYVATESSSFLNRFYNGTLNSMVLNFL
                     ENDKLSEDDIEELREILNKRTTKGDK"
     gene            15870..17669
                     /locus_tag="PC471_001939"
     CDS             15870..17669
                     /locus_tag="PC471_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaR1 family beta-lactam sensor/signal
                     transducer"
                     /protein_id="HDF2789069.1"
                     /translation="MYAPFFIRFLLSTLTLSILIAVILLAKKVFKKHISAKYQYNIGF
                     ILLIMLIIPFVPLKYINLGNVFNYLYTFNQLGSSTNRTSMIKNSPNSIINNSNALNDF
                     SVSLNQVNFDSLNILLLISWIIGILIMIAITIHCNIKIKNMKKSIQILRDEEIVFIFE
                     KCKKDIGINKDFILCKSPIINTPITFGYFKPHIILPDKSISKLSLKDIKYILLHELQH
                     FKNKDILINYIMCFLQILYWFNPLVWYAFKEMRIDREIACDISVLKKLDKDSHIEYGQ
                     TLINFVDNISRPSNLTLTSDIGGSKKQIKKRLLKIMDFHNESNTLKFKSILIFTLIAL
                     IVFGNIPKLSIMADINEKYRPTNEQTVYEDLSQYFKGFDGSFVMYDLKSKQYHIYNKE
                     KSKTRISPNSTYKIYSALLGLENGIITRNNSHIPWDGKHHSNSSWNEDQDLFSAMENS
                     VNWYFQTLDKKTGLKNIQTYLRQIGYGNYDVSGGISNFWLESSLKISPIEQVELLESF
                     YTNKFKFEDKNIETVKDSLLISKKDGVSLFGKTGTANINGKDVNGWFIGYVEKNENTY
                     FFATNIQSKDYSNGSSASKITLSILNDKKIYNP"
     gene            complement(18024..19898)
                     /locus_tag="PC471_001940"
     CDS             complement(18024..19898)
                     /locus_tag="PC471_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDF2789070.1"
                     /translation="MNKYNKIANELIKIIGEDNIISITHCATRLRVMVKDREIINDKK
                     VEKVDEVKGVFFTSGQYQIILGTGIVNKVYAEVEKMGLKTLSKKEQDELVKNNETGFK
                     KVMRTLADIFVPIIPVIAATGLFLGLKGCLFNDNVLGLFGMSSANIPLYIQTLVSVLT
                     ETAFAFLPAIIVWSAFKVFGGTPVIGLVIGLMLVSPILPNAYSVADPSNEIEAIMAFG
                     FIPIVGCQGSVLTAIVTAFIGANLEKWFRKHMPNVLDLIFTPFFVMLITMLVILLGVG
                     PIMHTIELKMVDIISLLIDLPLGIGGFIIGFTYPLAVITGLHHTYVMIETSLLANTGF
                     NALITLCAMYGFANIGTCLAFMKKSKNNQVKQTAVGAMLSQLFGISEPVLFGIQLRYN
                     LKPLIIMCASSGLGAAILSILHIQSNSYGLAVLPSYLMYIYDGYNLITYLLASIFVVA
                     FCFIVTCLFGVPKEAINEDEDEELVFNENNENFVSPAKGKIVALENVPDETFSKKMLG
                     DGFAIDIIDGKIVSPISGKLETVVSSGHAFGIKGTNGEVLIHVGIDTVALNGDGFDIA
                     VKQGDMVKQGDVLVNVDLKRIHELGKSTLTMVLFPDGKKVNILDINKDVKIGQRICVE
                     "
     gene            complement(19903..21567)
                     /locus_tag="PC471_001941"
     CDS             complement(19903..21567)
                     /locus_tag="PC471_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HDF2789071.1"
                     /translation="MEKKWWQKEIVYQIYPRSFQDSNNDGIGDLQGIISRLDYLKDLG
                     ITSLWLCPIFKSPMDDNGYDISDYLDIASEFGSMEDLKELIKKSKDRGIKIILDLVLN
                     HTSDEHPWFQEALKNPESPYHDYYIFKKGNDLPNNWRSVFGGSVWEKVENRDEYYFHS
                     FGKKQPDLNWENPILRSELYQMINTWSQMGVAGFRVDAITFIKKNLSFQSLESDGVDG
                     LVKCTKTVRNQPGIEKMLYELKENTFKKYDCMTVAEAAGISYDQLGDFIGEDGFFSMV
                     FDFKHADLDVASGSEWFKRISWTIPDLRKCILDSQMYVQEHGWAANFIENHDQPRATT
                     KYLLQYERNDDAVKMLGAMYFFLRGTPFIYQGQELGMTNFKRDTINDFNDLSSIDQYY
                     RSIDEGYSEEEALEFVNLRSRDNARTPFPWADKENAGFSDTTPWLKVIENYKDINAQS
                     QINNSESILEFYKKMIKLRQDSEYSDCLIYGSIEPIDIESDDIIAYRRQLDLVVIDCY
                     YNFSDKPVEIRVEEKYDIVFQNLDDINLLNKTVRLKPFHAVLLKVR"
     gene            complement(21581..22402)
                     /locus_tag="PC471_001942"
     CDS             complement(21581..22402)
                     /locus_tag="PC471_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDF2789072.1"
                     /translation="MKLYKLLILDIDGTLRDEVYGIPESAKHAIRLCQKNHCSVVICT
                     GRSMGTIQDDVLSLGVDGYIAGGGNYIQYHGELLYNQSFNQRLIKEVVCLLKNREVAF
                     SIESQEKVFMNQKAKEIFETMNQLKGTNSCINKQHIQEKITYENNIEEYKSQDIHKIC
                     LWSNEKVFDEVKDILQDKMELAQRDISSQYYEIIQKDFHKGKAIKRLQERLGVTQKET
                     ICFGDGQNDIVMFQASDVTIAMKNSHQQLKDIATSICEDIFDNGIYKELKRRNII"
     gene            complement(22501..23265)
                     /locus_tag="PC471_001943"
     CDS             complement(22501..23265)
                     /locus_tag="PC471_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDF2789073.1"
                     /translation="MKIEGLFNKYYNSFTANDKYICECILNHKEDCIKLSIDEFADKY
                     HISTSALSRFAQKLQLPGYSELRVIIRLNETEVEYKNQSKHQLMDCYNKVINDIEKKD
                     CSIMFERMKKANRIIVFGEGYSQERVAKEMKRIFLPTGKIIYDVYGYDMIQALNHFIK
                     TDDMIFFISLNGDASTIIDFAKEMRMKGIYMVSITKMISNPLARLCDENLYIQSISIS
                     VDKQLDYDVTTPYFILIELLYIKYKMYLESEHIPRK"
     gene            complement(23262..24011)
                     /locus_tag="PC471_001944"
     CDS             complement(23262..24011)
                     /locus_tag="PC471_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDF2789074.1"
                     /translation="MRLESLVNQNYDNLNENDKIIIKQILICQREYRNFSCEKIANEC
                     NVSRATLLRLCRKIGLDSFSDLKYLLKNNQLEFVNNDNNFHDVCENYRLLMNELQKIS
                     FQDICKIIYDAETIYIYGTGNEQKSLAQEIKRIFLSVGKCVIDLFDIGEIEFMRESFQ
                     KSDLFIIISLSGETKAGIEIAKFINNHIHTISLTRLDNNTIATLCEYNLYVETQKLDT
                     LQAISYEVVGAFYALLDLLHMNYIEYRRRKE"
     gene            complement(24515..25120)
                     /locus_tag="PC471_001945"
     CDS             complement(24515..25120)
                     /locus_tag="PC471_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDF2789075.1"
                     /translation="MKIKENVLMLDSTRGSNCFIVFDKEIILIDTGLPFMGKNIIKEL
                     KTLGIKLTDIKHILLTHHDVDHISNIKLLKEKTGAKVWAHVEDIPFIIGEKDRPGFKK
                     FIGKAISRRLIKDVEPYGENMQIGNIKIIHTPGHTPGHVCMIFEDVLFAGDLVKNKNG
                     NIVPYPNPWNWDYKKMMKSIRELDNLKYEWICMSHGTPCIK"
BASE COUNT         9030 a         4054 c         3064 g         9066 t
ORIGIN      
        1 cctttctatt tatccatttt caacttaaat tataagattt catcagctat atttttgcat
       61 accaataatt attaaatatc aaatatatat tctattaaaa tttctaataa tttcatcctt
      121 gatttattta atatataaaa atatactaac tttaatgatt aagttttgaa atataactaa
      181 gaatttgata atttattgag atttttatac taaaatatac atactataca aacttaattt
      241 agaagggagc attcttatga acaataaaat actcgtcgta gatgatgagg aaggtataat
      301 aacactctta aaagattact ttgaaatgaa tggttatgag gtttacactg ctcaaagtgg
      361 aaaagacgca ttaaaaaaaa tatctaaaaa tccagatatc attctacttg atataaacat
      421 gcctgaaata gatggaattg aagtttgtaa tagaattaga gattatgtat catgccctat
      481 tctatttctt acagctagaa ttgaaaatac tgacaagata aatggttttc gtgctggtgc
      541 tgatgattat attgtaaaac cttttgattt agacgaactt ggagctagag tatctgccca
      601 tttaagacgt gaacaaagga agtcaaatca aactaaaatt aaattttata atgatttaac
      661 aatagattac gaaaaaaggt taatttgtat atataataat cagatttcac tttcaaaaaa
      721 ggaatttgat atagtagagc ttctctctat gaatcctgga caagtatttg ataaagaaaa
      781 aatatatgat agagtctggg gatacaatag caatggaaat agtgatgtta taatggaaca
      841 cattagaaaa atacgaatca aactatcaaa acatactctt gaaaattata tagaaacagt
      901 ctggggagtt ggttataaat ggataaaata aataatatgt cattaaaaaa agctttcttt
      961 gttcttactt tatcttcact aattatagca tcagtgctta ctactttcgc atacatttta
     1021 cttaataatc tatataattc tatacaagat aagtatttaa aaacacctat aaatacaggt
     1081 attataacca tagttcaaaa caatgtgaac caatttagtg attatgataa aacattactt
     1141 aatataatca acatacttca gcttgtactt ccacttatct tttttatagg acttttatta
     1201 ttagctgata taatttttta caaggtaaaa ttaaaaacac ccatagaaat actcaataaa
     1261 ggtgcaatgg aaatatccaa caacaattta gacttttgct tagaatataa taataatgat
     1321 gaattaggta atctatgtaa tgcttttgaa aaaatgaggt ctgaactaaa taaaaacaac
     1381 ataaagatgt ggactatgat taatgataga aaacaactta atgctgcttt ttctcatgat
     1441 ttaagaaatc ccctaacagt tctaaaaggt tactcaaact atttaacaaa atacatacca
     1501 acaggtaagc ttagtgatga gaagatttta tcaaccacac aacttatgtc tgagcatatt
     1561 aacagaattg aatactatgt agaaaatatg agtaatgctc aacgattaga agatttagtt
     1621 gtttcaaaat ctatgtcgaa tataaacaag tttatagaaa acttagatga aaatatatct
     1681 atcattgcta aacaagaagg aaaatctttt acacttaaaa gtcaaataaa taatataaat
     1741 ctattttttg atgaaaatat cattcttaga gtcgtcgaaa atataatatc gaatgctttt
     1801 agatatgcta gaaataatgt atccatactt atatatcttg aacaagaact ccttaccttt
     1861 gtaattgaag atgatggtat aggatttagt aaggaatctc taaaattagc tttaagacct
     1921 ttctatagag ataagacttt aaatgacagc aatgcacact ttggtatggg tctttatatc
     1981 tcaaaaatat tatgcgaaaa acatggtggc tctatttcta ttgaaaataa ttctactaac
     2041 agtgctaaaa ttactgctaa attctcaaca agaaattaaa atgattttgt aagctttcac
     2101 tgttcttata taaaatttgt tagtttagaa tttataaaat gtatttcaat ttacaaccaa
     2161 tatatatact attttgtaga agttgtaaga gttgcttcaa ttataatatt tggcaaaagg
     2221 catttgaaat aataatagaa atatatattt aaatatgtag aatagtatga aattagaatt
     2281 ttggaggtga ttatattaaa agttttgcta ataatattac attctctcgt attgtgtttg
     2341 cattgctaat gcttagttct acattctctt cactgccttt ctggatatgt tatttttggt
     2401 gtggaattag tgatatactt gatggtctta ttgcccgaaa gttgagacaa caaagtggaa
     2461 taggtgcaaa gttagatagt attgctgatt tcatttttgc ggttacaatt ttggttactg
     2521 tcggaaaaaa tattgctctt cccatgtatc tatggtggtg tattgttgca atagcattgt
     2581 tacggttgat tagttacagt attggttttt ttaagtatcg cacattttca tcgcttcata
     2641 ctttgatgaa taaaatcact ggaatttcta tctttgcttt tccattactc tatatttgct
     2701 ttggtcttga tgttgcagga attatgatat gtatcgtggc ttttctttct tcttttgaag
     2761 aaatagcaat tatgataaaa tcaaaagaat taaatcgtga tataaaaagt atatttatgt
     2821 gacaacttag aatttgtgac aaaaataatt tatataataa tgaggaagta aaacataaaa
     2881 aattatccac aagtttatgt gtaaaatagt tttaaagcaa tagagttgta ttgtaaagcc
     2941 tttagtgcat aaattacatt ttaaattaaa aatgatactg aaactgctta tcagcactgc
     3001 gaattatccg caaatagtga agacttttta gatttaagct aaaagttggg aaaaaattga
     3061 gttttaaaac ttacactctt cttatataga aaaggagagt gttttttatg attataaaag
     3121 caaaacaatt atcaaaaata tatggttcta ataacaataa agttatagca cttaataatg
     3181 taaatttaga aataaactca ggtgaatttg tttctgtaat tggaccttct ggaagtggaa
     3241 aaagcacact tttgcatatt ttaagtggat tagataatcc tacctctgga caagtgttac
     3301 ttgatgataa agatatatat aaacacactg aaaaagaact atctgcactt agaagaaaat
     3361 cctttggatt cgtctttcaa caatttaatc ttttacctgt tcttacagca tctgaaaata
     3421 tttcaatgcc tgtacttcta gataaaaaac aacctgataa agactattta aatgaaattt
     3481 catcattact tggaattgca gatagactca atcatcttcc ccatgaactt tcaggcggtc
     3541 agcaacaaag agttgcaatt gctagagcac ttattgcaaa accagatata atatttgctg
     3601 atgagcctac aggaaacttg gacagtaaaa gtggcagtga agttatgaat cttcttataa
     3661 aaacttcaaa acagtttgga aagactttag tggtaattac tcatgatgat agaattgcaa
     3721 aacttgctga tagaaaaatt tcaattattg atggtgttct tatggaggtg aaatagaaat
     3781 gaagaattac ctttcacttt caataaaaga attaaaaagc cagaaattta tgactacatt
     3841 catcattata gcaatagtac tatcaactat tatgacaact gtggttggac agtcaattgg
     3901 tatcctacaa aatttaagaa ttgaacaagc ccgttctttt aatggtgata gacatgtatc
     3961 ctttcaccaa ctcactaaaa atcaagttga tgacataaag aaagatgata gagtttatca
     4021 agctggtact tcaataacta ttggcagtag taaaataaag gattctggta tctctgtact
     4081 tgtaaaagag tatgataaaa caggactttc taactaccca aaattaatga aattaaaatc
     4141 tggacatttg cctaaagata aaaacgaaat agccttagat gaaaacacac taaagcttat
     4201 gggtataaaa ccaagactag gagtgactat acctatgaat ttagatattt cacttttaaa
     4261 tgataccata ccaccatata attacacagc taactttaaa ttgagtggca ttttagagga
     4321 tgattataca ggatatgtaa gtggtatagt aaatggtatt gtaggtaaag gtacatcaga
     4381 aaatctatta cctaagagat atattttatc ctctttggat tttaaaataa aacagcaaga
     4441 aaagtttcaa gaaatagtaa atcaattagc taaaaaaata aatctttctc acaattcaat
     4501 tcaatataat tggatttact taaatgctct taagatacag tttgaaaaag atgaaaatag
     4561 ttctaatagt gatggtatat ctatgattat attggtatct ttatttgttg ccttattggt
     4621 tttactggct tctggacttg taatttataa tattttaaaa atctctgtta caaagaaaat
     4681 taaagagtat ggatgtttaa gagcaatagg agctgaacca aaccagatat ataagatagt
     4741 tattttacaa atcttaattt tatgtactgt tgccatacct attggagctg taattggaat
     4801 tatatcttct aaaggcataa ctggaatggt cactaatatc ttaaatccag atatacttct
     4861 agcaaatgat aataaagaaa ttactgagct tatacataaa aatacaacag catatatatt
     4921 cccattagta ttaagcacta atgtatcttt gattttttca tttatttcag cattaccatc
     4981 agcaatatat gcttcacatg tctctcccaa aattgctatg gttggtagta ctaccaaaat
     5041 caaaagaaaa attaaaagag aaaaaacaat aaaaaacttt gaaagacatc tagcatggct
     5101 aaacttaaaa agaaataaag gaagaactat aataactata ctatcattat ttatgagtat
     5161 aacagtattc gttgcactga gtgagttctc taatgtatta gatgtatcta ggtcagtaag
     5221 taatttaaaa gaaggagatt tttccttaac aaatgaaata tctggttttg ataaatcaac
     5281 actagataaa atagctaaaa tgaaaaacgt caatcgcact tcctttataa agtactctga
     5341 atacaaacag ggtgaaatag atacagatat aaattttgaa aatagtggtg agatgttaaa
     5401 aatcattgga attgatgagc aaactttaaa agacttaatg ccttctatta cagattctat
     5461 actagaagac tttaaaaatg gttctatctg ttttataaaa aatccgattg ctatttctac
     5521 accaggtgta aaaactagat atacaaattt aaaacctaaa gatagtatca caatcaacaa
     5581 aaaacaacta gatatatata gtacagtaga taaaatgttc tttttacaag gcaatggctg
     5641 gataaatggt gttgatgtta ttgtatatga ttctgtttat aacactttga caaataaaaa
     5701 caagttcaac cagataaata tttatgctaa agataaatca aaattagaac aaataagatt
     5761 gtctattgaa cagatttgtg aaaataatcc tggctctcat tggatatcat atattgaatc
     5821 agataaacaa cttaaagaga gttttaaaca aatagaattt ctagcatggg cagttatatt
     5881 atttgtggga ttgattggta ctttaaacat cattaacaca actcatacaa atataaatac
     5941 taggactaat gagattggag ttaaaagagc tattggtatg agtaatagga gcttatataa
     6001 aatgttttta tgggaaggtg tttattatgg aatttttgct gctatatttg gttcaattgc
     6061 tgggtatgcc agtgctatta tcataaacat ggcaactata gaaaagcttg atttcactaa
     6121 tataccaatt acttcaattt tacaagctac tattatttct gtacttgcat gtattatagc
     6181 tactcttatt ccgttaagaa aagttaaaaa gatgaatata attgactgta tagatatcaa
     6241 tttataacta ttttacagat aataatataa attaaattat caatgactgt ctctattttc
     6301 tttcaaatct aactaataaa tctttttaac tataatataa gctttaaaaa aggcctgtct
     6361 caaaatagag ataagccttt tttcattgtc gaaataatga aaatatattt aacttcacaa
     6421 aaaaagagtg cctcacgaat taaaaatttc gctttgagac actctccttt aatttaaaat
     6481 atatagttaa atgctatgcc aaaatttcac acatgctcca cctgattttg aattttctat
     6541 tttaacagaa cctccaagct gttccactaa ttgtttgaca atgtaaagcc caagaccaga
     6601 atgtccacct tttgtctgcc tagcttcatc tcctcgataa aatttatcaa gagccttttt
     6661 taaatcttta gagctaaacc ctctaccaga atcacaaatc tcataagaaa tacaattttt
     6721 ttcatccttt acagtaatac taatattacc acctgatgga gtgtattgca gactgttgga
     6781 tatgatatta tcaaaaattc gtgtcaacct atcaacgtcg atttgaattg gggattgtat
     6841 attcccctgc attttcaata taagttcaac ttccttttga tgagcctgta attcatagtc
     6901 ttgcaccttt tgcctcaaaa attcaggtaa atttatagga acaagattta attgtatatt
     6961 tgacttttct aagtcagacg tatattgcat cttttggaca agagcagcac ttttttctgt
     7021 attttctctt atcactgtaa gatacctatg aagtttttca ttgtcatctg tattattacc
     7081 aataagtgca tctgtatatc ccaaaataat agaaagagga gattttaaat catgtgctag
     7141 agcttccacc atttcaacac gttcctgctc catcttccac tgtgcagata aagagccttt
     7201 taattcgtct ttcatttcag aaaaggcact acaaagttta ccgagttcat tatctgaata
     7261 atagtcgatt tcaaaatcaa ggtccttttc ttttatcttc ttggacgcat caatcaatag
     7321 atgtaatggt tgattgatat tctttacaaa gcgcttagaa agccatctag taaaacccac
     7381 gatatataga aatggagaaa acaaagccat tataataact gcaaatacaa atctccctct
     7441 aatatttgca aaggtgattt tcacttggta gaatagaata acagcaccct caatctttcc
     7501 attatcccct ttgattggaa cagtatggat ataaccacct ttacgaacag ttttattgat
     7561 aaaattatta aataattctt cttttgtttt aaatagtttt ttggggttag taccatataa
     7621 aatattgcca ttattatcca caacttgata tagcatatca tccccacgaa tcgttctttt
     7681 taaaccttct tcattcgatt gtgataacaa agctatgttt ttttcattga tgtatttttc
     7741 aattctagga acttgtcgct cataatagtt tggtggatag atatccttat ttaaggaatg
     7801 aatatacaaa tagagagcaa atacataagt aatcactgtg gcaactatac tggctattat
     7861 aataaaaata aaagtatgtc taaattgggt aataagggat tgatttttca tttaataacc
     7921 tctctctctt tacattttat tccacttata cccaataccc caaactgtgg aaatatattc
     7981 tttttctggt gtcacagcag aaaacttagc tcgtattttt tttactcgtt caacaacagt
     8041 tgaggaatca ccttcagaat cacaccccca tactttttca tagatttgtt ctcttgagaa
     8101 aacttgtcct gcatggagtg ctagtaattc aacaatatca tactctattt ttgttaaatc
     8161 aatattctta ccatcaattt taattgcacg ttccttcata tataagcata acttcccaaa
     8221 atacaaagta gatctcttgt tctcttcatt gatatactgt gaacgctttt ccctacgcag
     8281 attagcttca atccttgcca taagttcacg cagtccaaat ggctttgtta cataatcatc
     8341 ccctccaaag gtcaaccctt tgattttgtc ggtttctgat tgctttgcac ttaaaaatat
     8401 aattgggcat aaaacatcat ctctaattgc ctgacatacc tcaaatccat tcattttagg
     8461 catcataata tccagtataa taaggtcagg ctcctgtttt gataactcta tacctgcctg
     8521 accatcataa gctatcaaca cttcatgtcc ttttgccttc aattcatcct caagtagcat
     8581 tactaggtcc atttcatcat caataatcaa tattttactc aatataacac cacctttctc
     8641 ttatgggaag gtcagtatta aaacaatatt cgtctactca taacttttcc ttccctccca
     8701 cattttaaac caaataacgc ctcctatcaa gaatataatc aaacctattg ctactactat
     8761 aagctctata ctagctgttt gcatcacttt agagtaaaat gcctcagttg aaatagttcc
     8821 agcttcttta ataaaataaa ttcctggaag catacctagt tttactggcc atgtccatgg
     8881 aacaaatgcc catacttttt caccaacgct agttaatcca agtattgctg ccatcaaaat
     8941 tccaccaata ctaataccaa tagatgcacc cataccccat gcaaaactca cccaaagatg
     9001 aatagctaat agtggaagtg tcccaatcac cacaagtcct gctgctgaca ggaataacca
     9061 tattgttgct ccagatggca ctgcaatact cattcctatg caaagtatca acgtagcaat
     9121 aaatgtgcat atagttaggc aaaacagaag aaaaagaaat tttcccagat ataatctgac
     9181 acgagaaata cctacacata aaaaaccatt aaaatttcca gcaagctctt cctcctgcac
     9241 tataaatcct gctaaaatac ctactccaat aggaatgatg aatacagtcc atactgtaaa
     9301 aaatccttca aaagcaaatt cttgtgtact tccagattta gttgtgagat aaaaaaccac
     9361 acataatgaa aaaataatag gcatacaaag agtaagccac tggacagctg tcctctttgc
     9421 tcgaagccat tctgatgata atagttttat catttgtcat ccctcctatt gaaccaagct
     9481 gcagtaagtg ctgttaatac tataaacaca accaaagaaa caacaatccc aataggaatc
     9541 actgaagtat tcaaaagtgg gtctccagct tctaatatag taccgttagg atgtatgttg
     9601 atgattgggc ataccattct aactgcccaa ctccaaggta ctgcaatcca aaatggtttt
     9661 gtcgcagcaa gaacaccaaa tatcatacct aaagctccaa ctcccatact taggaacata
     9721 cctttccatg tagctagcca tagctgaagt ggaagtatag caagagaaac aacccagcat
     9781 acaatacttg cccctataat ctgtccaaaa ggaactggac cagctttcaa tattaaacca
     9841 gttataattg ttacgactat taaaactagc gttgaaataa aactgtagac tgccatagct
     9901 ataactttat taatccaaag tgtcatagga gacacattgt gtgtacgtaa agcacgataa
     9961 tttccagctt ttttttcctg gaaagctacc aaagtagcga acaatgcaaa tccaataggc
    10021 agaaataaaa atgaccacca gttaaaaatt aatgctaaaa gattttgcca cgcccaagaa
    10081 gttgtttgag ataatggatt ttgcatgagt gttgattgca ttataaatgc atatgctgca
    10141 aaagacactg ggaaaaaaac aataagttta cccataaatg ttcttttata ctttaaaagt
    10201 tctgattgta aagcattcat ctgttttgtc ctcctttaag aataacatct gtaaaaaatt
    10261 cttctagatt ttcattaggg tcaggcattc cttgaaataa tagtcgtcct ccatttataa
    10321 ttcctatatt atctacaact tgagcaacct ctgatagaat atggctagaa agaattacag
    10381 ttataccttt tttgggaaaa gaagcaatca attcacgcaa ttcttgaatg ccaaaggggt
    10441 caagaccatt ggttggttca tctagtatta acaactctgg gtcactcaaa agtgcaatag
    10501 ctattcctaa cctttgtttc atacccatag aaaattgtga agctctcttt ttacctgtat
    10561 ttttcaaatt tactatttcc aacacttcat aaattttatc tttaggtatt cccaatattt
    10621 ttgtatgtac taacaaattt tcctctgctg taaggtttcc atacaatgca ggggattcta
    10681 ttaaagaacc tatttttgca agagattctc gtttccaagg ctttctatca aaaataatac
    10741 gaccatttgt tgggcgaaga agaccaacaa gcattttaag tgttgtggac tttcctgctc
    10801 catttggtcc taaaagtcca tatatactat ttcgttcaat ttgtagagaa acattgttaa
    10861 ctgccatttg ttctccatat ctttttgata agttttttgt ttcaagaatt aaatctttca
    10921 tattaatttc tcccttcttg taatacctta aatctgttac taagagaata ctaacatgaa
    10981 aaaataacaa ttctataaca cctactaatt ttaataaaat ttaaaataaa aaatagagtt
    11041 accaatttta ttttggcaac tctatttaag tttatgtttt acagttatat tagatttaaa
    11101 cttagtttca ttatcacact aacttcaaat ttactctata atctttaaat caaacactaa
    11161 tttattaatc aaacactaat ctattaattt atatttcttt agacatctaa tatagtttgc
    11221 tactaaataa taattattta tatcatattt atctaatcct ttaaaagaag ttatttcatc
    11281 attaagctca tcactacctt ctacaaaaac acaacctgtt tcaaattcca tacctacaat
    11341 aatcttacag tcactataaa tttcctcttg agtatcgtac aatttttttg catttccttc
    11401 atttggaatt gtaaatacaa taggttttaa ttctttatca ctggaaatct tttgtgaaaa
    11461 aggcttgttt aacttaatat tattgataac tacatgttta aattcctctg aagtttcttc
    11521 tactattaca tattttcgct ttaaaaactc tattatttct aaaacagact tagaatttac
    11581 ttttaaaata cttttatatt tttcatataa agatttccac tgttcaattt tttcctgtgg
    11641 tatttcttgg atatgtgtac ttattttttc ttgagatatt tttttcaatt aaactctcct
    11701 ttcaatactt aaatatcctt atgaatctca catgactaaa gttacaaggt ttctacttaa
    11761 tagaattttg catacttaaa tatgtcttat taattctcta caggatatct ccgtagttcc
    11821 tacgattctt acataagtta cttattttaa attgttatta gttatattct aaaaattatt
    11881 tattttatca ctaatataat actcttaaaa actttacaaa tcaatttaaa tttgctacct
    11941 tttatttata acaacttata gtacatttct cctatgcttt tcaatatatt acttttaaaa
    12001 ggaggttttc attatgattt taaatagagc tgagcttttc cgaggatatt ctttaaatag
    12061 atttaacgag atatgcaata tattatcagt aaataaaatt aaatataagt ataaagtgaa
    12121 aaataatgct tactctaaaa aacctttttt taatgaaata acacttggca atttaggcca
    12181 aaaggaagat ttctcttatg agtacttaat ttatgttcat aaagacgatt atgaacaggc
    12241 agagtctcta ataaattcta agttgcgata gattatctat aaaacatgac attctgttgt
    12301 catgttttat atgttattat actttcaaat atgctactat attttcaaaa ttgtgtgctt
    12361 taaaactagc tttttttagt aatttaaagc ttaaaactaa ggagactcgt tatatgaaaa
    12421 taaatataaa atcaaagtta agtgaattta ttaaacagaa caatcttttt gatgacagca
    12481 gaataatatc ctatcttcct aatataacaa tagaaacaga caaaataaaa actataatga
    12541 ttaatgaggt tgtcccaact aacaccaatg acgattttta tagtgtagat aaagatgctg
    12601 attatttaaa aactactatt cctttatttg acagtgcagg aataaaagta tctaacataa
    12661 atgatatcct agatatgggt atctatataa ctaatgcagt aaaacttcct aaatcagaat
    12721 atacaataac tcgtgataca attaaattac atatgcctat tttagaagaa gaaattaaat
    12781 tatttaaaaa cctagaggta gtaatgttaa tgggagatgt tgcaaaaaaa tcttttaata
    12841 tgattactaa aaagcatata aaaaagaatg tcatcccttc catttcaaca tataaacttc
    12901 gtaataatga actttattat gataatttaa gagtatttcc atcatatatt atgactggtg
    12961 gaaacatact gattgaaaaa tcaaaatttg aaatggcatc agaagatata aaaaaaatgt
    13021 tcgagattat aaacggttga tattacaaaa taataaaaac taatataaaa taaaaagtgc
    13081 ctcaaaaact tctatttatg aggcactttt attttatatt agtgtaacta tatactatta
    13141 acataattta ttatttatat tgttcttttt aatctctatt ccatttagtc taggtaacat
    13201 tactctttac aagattactt tatatttctt ttatatctct aacgttttta tttaataaaa
    13261 caattattaa tacaagtcaa atatatctat ttatctaaaa atatatcttt tacagtaata
    13321 tctggatggt gatacttctt cctattcgtt aaaaaagatt tttttacatt aatagcctct
    13381 tttggacacc agtgaaaaca agctaggcat ctttcacact cattacccca ctttggcttt
    13441 ttatcaacta tacttatatt ctctacagga caaacccttt tacatgtccc acatccatta
    13501 catttatttt ctgcaaaaaa tttagtagtt gcaatttgca tattcttacc tactaaacga
    13561 agtacaacac tatctatatg tgactttaaa ttgttttttc cttctatatt tccaatctcc
    13621 aagttattga tacattttgc aatctcagct accctattag tctgattttt caatctttct
    13681 tcttcaacat ctggaggtgt tatcaaagca tttccaggca tattgattaa aaaaccagca
    13741 gataattttt tccccctttt tttaagtaac ttatctatag taaataaact atgtccagct
    13801 accccattac aagtggcaat ggcatatata tgagtattaa tattaaaatt taatttctct
    13861 acaaaagact ttactatatc aggaacatca gcaaaataaa caggaaaaac aattaccaat
    13921 atatcttcat ttatgtctat attattttct tttctatgta tagctaaggg tataacttct
    13981 ttacttccat ttaacgtttc ttttaaaata tttgaaacta ctagcgaatt tcctgttcca
    14041 gaaaaataat aaatcttact attcattctt atccctcttt ctatatttct acatcatcaa
    14101 tagttaaaaa gtctcttaaa ataggattta tatttacttt ccaattctat ccaaaatatc
    14161 agtaaacaaa ggcaattctt catcagagat agatttaaat gtctctgttg catatatact
    14221 tgcaatttca tttaatggtt cttctaattc tctacctaaa tctgttagga atatttgaaa
    14281 cttacgtcta tctttaacat ctactttacg gacaattaat cccttatttt tcataacatc
    14341 taaaattctt gttgtattag atttatcctt acatgatttt tcacataaat ctatctgaga
    14401 aatcccatct tgctcccata gaaaccttaa taacgcataa tggtcagttg ttatatcata
    14461 tccattttgt ttaaacatct tttttaaatc atcttttact tttaacgaag tacttactaa
    14521 cttagttgta gcttcatata gtttctcaaa atctttcatt ttacctccat ttaaataatc
    14581 ccttgctcct cccatctatc aattattccc ttatattgct caatatcttt aaaagccatt
    14641 ggctttaaaa cagcatttat agcaataaaa ttatagattt gagaaagcac aggtggattt
    14701 ttcttttcct ctatgcaata aataaatttt ttagatacct tttgtatctt tgattctact
    14761 ttttcagtta taggattttg attaaatcca gtttttatag ataaactacc acaaacataa
    14821 cctcccgaat ctaagaccat ggattttata aaagaaattg cttttgaacc acgtgcattt
    14881 tcatatgtag taactgctat acatggctta cgatacaata atctttcaag catcatatgt
    14941 gctctatctg taaacacctt gaaaagtcca gttacactac ttccataagt tgggctacca
    15001 aatataacac catcactctt agtaattata tcatgaatat actctacttt atcattttga
    15061 ttgatacact tgcccatttt ataacaattt aaacaaccaa tacaatatga tggatttacc
    15121 tcacttaaat caaaataatt aatttttaca tctggtatta atctttctat atctttatac
    15181 aagtaagtta aaacttttga tgtagctcca ttttttctag ggcttccatt tataactgta
    15241 attatcattt ctatatctcc tttttagttg ttatatcaac aataagtata tcaactataa
    15301 ttttagctgt caatataaac catttattta aagctatact tacacagctt aaataaaagt
    15361 tttatttaac tacatgtgct tatttaatca actataaatg aattattttt aataattgac
    15421 aaactaaatt aatgataata tactttatgt attacaaatg taagtagaag gaggtatagt
    15481 atgaaaaagt taccacatat ttcagaagca gaatatcaag ttatgaaaat tatttggaaa
    15541 tatgctccta tcagcactac tgaagtaatt gaaaagttgg tagaaacaag tacatggagt
    15601 cctaaaacaa ttcaaacaat gttattgaga ttagttaaaa aaggagcttt aacatatgaa
    15661 aaaaatagca gagtattcgt ttatactcca cttgttaaag aagaagaata cgtagctaca
    15721 gaaagtagtt cttttttaaa tcgtttttat aatggtactc ttaattctat ggtactaaac
    15781 tttttagaaa atgataaact atctgaagat gatattgagg aactgagaga aatcttaaat
    15841 aaaaggacga ctaaggggga taaataaata tgtatgcccc attctttata cgatttttac
    15901 tcagtacatt aaccttatca atattaattg ctgtaatttt acttgccaaa aaagtattta
    15961 aaaaacatat ttctgcaaag taccaataca atattggatt tatattgctt ataatgttaa
    16021 taattccatt tgtccctctt aaatatatta atcttggaaa tgtatttaac tatttatata
    16081 cttttaatca acttggaagc agtactaata ggacttctat gataaaaaat agtccaaatt
    16141 ctataattaa taactcaaat gcattaaacg atttttcagt atccctaaac caagttaatt
    16201 ttgatagttt aaatatttta ttactaatat catggataat tggaatttta atcatgatag
    16261 ctattactat tcattgcaac attaaaataa aaaatatgaa aaaatcaata caaattttaa
    16321 gagatgagga gatagtattt atatttgaaa agtgtaaaaa agatattggt ataaataagg
    16381 attttatact atgcaaatca cctattataa atacacctat tacatttgga tactttaaac
    16441 cacacattat attaccagat aagtctatat ctaaattatc tttaaaagat ataaaatata
    16501 tcttacttca tgaattacaa cactttaaaa ataaagatat actgataaat tatataatgt
    16561 gcttccttca aatattatat tggtttaatc ctcttgtatg gtatgcattt aaagaaatgc
    16621 gtatagaccg tgaaattgca tgtgatattt cagttttaaa aaagcttgat aaagatagtc
    16681 atatagaata tggacaaact ctaataaact ttgtggataa tatttcaaga ccatcaaatc
    16741 ttacacttac atcagatatt ggtgggtcaa aaaaacaaat aaaaaagcga ctattgaaga
    16801 taatggattt tcataatgaa tcaaatacgt taaaattcaa aagcatttta atttttacat
    16861 tgatagcttt aattgtattt ggaaatattc ccaaactatc tattatggca gatatcaatg
    16921 aaaaatatag acctacaaat gaacaaactg tgtacgagga cttaagtcag tattttaaag
    16981 gttttgatgg aagttttgtt atgtatgatt taaaatccaa gcaatatcat atttacaata
    17041 aagaaaaaag taaaacaaga atttctccaa attctactta taagatttat agtgccttat
    17101 tgggattgga aaatgggata ataacaagaa ataattcaca tatcccttgg gatggaaagc
    17161 atcattctaa tagttcttgg aatgaagacc aagacttatt ttctgctatg gaaaactccg
    17221 taaactggta ctttcagact ctagataaaa aaacagggct aaaaaacata caaacttatc
    17281 ttaggcaaat tggatatggc aattatgatg tttcaggtgg tatctcaaac ttttggctag
    17341 aatcttcatt aaaaatatca cctattgaac aggtagaatt acttgaaagt ttttatacaa
    17401 ataagtttaa atttgaagat aaaaatatag aaacagtaaa agattcattg ttaatatcaa
    17461 aaaaggatgg ggtatcactt tttggtaaaa caggcactgc caatataaat ggaaaagatg
    17521 taaatggttg gtttataggt tatgtggaaa aaaatgaaaa tacttatttc tttgctacaa
    17581 atattcaaag caaagattat tctaatggta gttcagcatc taagataaca ttatccatat
    17641 taaatgacaa aaaaatctat aatccttaat atatttactg tacattctaa aaaattcaca
    17701 taattttcat attaattatc aattaaattc atatacaagg cttattatat aaacatagaa
    17761 gatagtaaaa ctagcttaaa aacataatat aataccctat acaaatagtg gcaactctcc
    17821 ctcacatcct caattgttgc cactatttaa ttttttgtat gaagttaaat atattttcat
    17881 catttcaaca atttgggtgc aatacaatct ctattttgaa acaacctctt taattgtttt
    17941 agagtttatg aattatttct catactttaa aaagattgaa ttactcttga taatcttctt
    18001 ttgtagtcat tacatatcac ctactactca acacatattc tttgacctat ttttacatcc
    18061 ttgtttatat ccaaaatatt taccttctta ccatctggaa acaataccat tgttaaagta
    18121 gactttccta attcatgtat tctctttaaa tcaacattaa caagaacatc tccctgttta
    18181 accatatctc cttgtttaac agcaatatca aagccatctc catttaatgc aactgtatca
    18241 attccaacat gaattaatac ttctccatta gttcctttaa tcccaaatgc atgtccactt
    18301 gaaaccactg tttctaattt acctgaaata ggagaaacaa tttttccatc tataatatca
    18361 atagcaaaac catcacctaa cattttttta gaaaatgttt catctggtac attttctaat
    18421 gcaacaattt tccccttagc tggagacaca aagttttcat tattttcatt aaacactaat
    18481 tcttcgtctt catcttcatt tatagcttct ttaggcactc caaataaaca tgttacaata
    18541 aagcagaatg caaccacaaa tattgatgct aataagtatg tgattaaatt atatccatca
    18601 tagatataca ttaaataaga aggtaaaact gctaatccat atgaattaga ttgaatatgt
    18661 aaaattgata atattgctgc tcctaaacct gaagatgcac acataataat caaaggcttt
    18721 aaattatatc gaagctgaat cccaaataaa acaggctcac ttataccaaa aagttgagat
    18781 agcattgctc caactgcagt ttgtttaact tgattatttt tagatttttt cataaatgct
    18841 agacatgttc caatattcgc aaatccatac attgcacaaa gagtaataag ggcattaaat
    18901 cctgtatttg ctaatagtga tgtttcaatc attacataag tatgatgtaa gccagtaata
    18961 acagctaatg gatatgtaaa tccaataata aatcctccaa ttcctaaagg caaatcaatc
    19021 aataagctga tgatatcaac catttttaat tctattgtat gcattatagg gcctacacct
    19081 aacaaaataa ctaacattgt tattagcata acaaaaaatg gtgtgaaaat aaggtctaat
    19141 acattaggca tatgtttacg gaaccatttt tcaagattag ctccaataaa tgctgtaaca
    19201 atagctgtta aaacacttcc ttgacaacct acaataggaa taaatccaaa tgccataatt
    19261 gcttcaattt catttgatgg gtcagcaaca gaatatgcat taggaagtat tggggaaact
    19321 aacataagtc caataacaag tccaataaca ggtgttcctc caaatacttt aaatgctgac
    19381 catacaataa ttgctggtaa aaaagcaaat gctgtttctg taagaaccga aacaagtgtc
    19441 tgaatataca atggaatatt tgcagaagac attccaaata atccaagtac attatcatta
    19501 aacaaacaac cctttaatcc caagaataat cctgttgctg caataactgg aataatagga
    19561 acaaatatat ctgctagagt tctcataact ttcttaaatc cagtttcatt atttttaact
    19621 aattcatctt gttctttttt tgataaagtt tttaatccca ttttttcaac ttctgcataa
    19681 actttattaa ctattcctgt tcctaaaata atttgatact gtccagaagt aaagaaaacc
    19741 cctttaacct catcaacttt ttcaactttc ttatcattaa taatttctct atctttaacc
    19801 atcactctta atcttgtggc acaatgtgta atagaaataa tattatcttc accaataatt
    19861 ttaataagct catttgctat tttattatat ttattcattt ttctacctca ctttcaataa
    19921 tactgcatga aatggtttca aacgcactgt tttatttaat agatttatat catctaaatt
    19981 ttggaataca atatcatatt tttcttctac tcttatttct acaggtttat cagaaaaatt
    20041 ataataacaa tcaataacta ccaaatctaa ttgccttcta tacgcaataa tatcatcact
    20101 ttctatatca ataggttcta ttgaaccata aatcaaacaa tcagaatact cactatcttg
    20161 tctcaacttt atcatctttt tataaaattc taaaatactc tctgaattgt taatctgact
    20221 ctgtgcatta atatctttat aattttcaat aacctttaac catggtgttg tatctgaaaa
    20281 acctgcattt tctttatctg cccatggaaa tggcgttctt gcattatctc ggcttcttaa
    20341 attcacaaat tctaacgctt cttcttctga gtaaccttca tctattgaac gatagtattg
    20401 gtcaatgcta gataaatcat taaaatcatt tatggtatct cttttaaaat ttgtcattcc
    20461 taattcctgt ccctgataaa taaatggggt tcctcttaaa aagaaataca ttgctcccaa
    20521 catttttact gcatcatcat tcctttcgta ttgcaaaaga tactttgttg tagctcttgg
    20581 ttggtcatga ttttcaataa aattagctgc ccatccatgt tcctgaacat acatttgact
    20641 atctaaaatg cattttctta aatctggaat tgtccatgaa attcttttaa accactcact
    20701 accagaagca acatctaagt cagcatgttt aaagtcaaag accattgaaa agaaaccatc
    20761 ttctccaata aaatcaccta actggtcata gctaatacct gctgcttcag caacagtcat
    20821 acaatcatat tttttaaacg tattttcttt taattcataa agcatttttt ctattccagg
    20881 ctgatttcta actgtttttg tacatttaac aagaccatca acaccatcac tttctaaact
    20941 ttgaaaactt aaattctttt tgatgaatgt aatagcatca actctaaatc ctgccactcc
    21001 catttgagac caggtattaa tcatttggta aagttcactt cttaatattg gattttccca
    21061 attaaggtca ggttgttttt ttccaaaaga atgaaaataa tattcgtctc tattctctac
    21121 cttttcccaa acacttccac caaaaacaga cctccagtta tttggcaaat cgtttccttt
    21181 tttaaaaata taataatcat gataaggact ttctggattt tttaatgctt cttgaaacca
    21241 aggatgttca tcacttgtat gattaagaac taaatctaat ataattttga ttcctctatc
    21301 tttacttttt ttaattaact cttttaaatc ttccatactt ccaaattcac ttgcaatatc
    21361 caaataatca ctaatatcat acccattatc atccataggt gatttaaaaa ttggacataa
    21421 ccacaatgat gtaataccta aatcttttaa ataatctaaa cgagaaataa ttccctgtaa
    21481 atctccaatt ccatcattat tactatcttg aaagcttctt ggataaattt gataaacgat
    21541 ttctttttgc caccatttct tttccataaa ttactccttt ttatatgata tttcttcttt
    21601 ttaattcttt ataaattcca ttatcgaata tgtcctcaca tatagatgtc gctatatctt
    21661 taagttgttg atgactattt ttcattgcaa ttgtaacgtc tgatgcttga aacatgacaa
    21721 tgtcattttg accatcacca aaacatatgg tttctttttg agttactcct aatctttctt
    21781 gtaatctctt aattgctttg cccttatgaa aatctttttg aataatttca taatactgac
    21841 tagaaatatc tctctgtgct aattccattt tatcttgtag tatatctttc acttcgtcaa
    21901 ataccttttc atttgaccat aaacaaattt tatgtatatc ttgtgactta tactcttcaa
    21961 tgttattttc atatgttatt ttttcttgga tatgttgttt atttatacaa gaattagttc
    22021 ctttcaactg attcattgtt tcaaatattt ctttggcttt ttgattcatg aaaacttttt
    22081 cctgactctc aatcgaaaaa gcaacttctc tattttttaa taaacaaaca acttccttta
    22141 tcaatctttg attaaaagat tgattataaa gtaattcacc atgatattga atgtaattac
    22201 ctcctcctgc aatatatcca tcaactccta atgataaaac atcatcttgt attgtaccca
    22261 tacttcttcc agtacaaata acaacactgc aatgattctt ttgacataaa cgtatagcat
    22321 gctttgcaga ttctgggatt ccatatacct catctctaag cgtaccatct atatcaagta
    22381 ttaataattt gtacaatttc atcttccttt cttactatca aaatcataat agcattcaaa
    22441 aaaaaagata tcaatatttt gatatctttt aaaaaatatg ttcattttta gcacaaattt
    22501 tcattttcgg ggaatatgtt cactttctaa atacatcttg tatttaatat aaagcagttc
    22561 aattaaaata aaataaggtg ttgttacatc gtagtccagt tgtttatcta cagaaataga
    22621 tatagattga atatacaggt tttcatcaca taatcttgct aaaggattac ttatcatttt
    22681 tgtaatagaa accatataaa ttcctttcat tctcatttct ttagcaaaat caataattgt
    22741 agatgcatct ccattcaatg aaataaagaa aatcatatca tctgttttta taaaatgatt
    22801 aagtgcctga atcatatcat aaccataaac atcatatata attttccctg ttggtaagaa
    22861 aatccttttc atctctttag caactctttc ctgagaataa ccttctccaa agacgattat
    22921 acgatttgcc tttttcattc tttcaaacat aatcgaacaa tccttcttct ctatatcatt
    22981 tatcacctta ttatagcaat ccattaattg gtgtttactt tgatttttat attctacttc
    23041 tgtttcattt aagcgtataa taactcttaa ttcactatat ccaggtaatt gtagtttttg
    23101 agcaaaacga gaaagtgcac ttgtagatat atggtatttg tctgcaaatt catctataga
    23161 aagtttaata caatcttctt tatgattaag aatacactca cagatatact tatcatttgc
    23221 tgtaaatgaa ttataatatt tattgaaaag tccttcaatt ttcattcttt tctccttcta
    23281 tactctatgt aattcatatg aagcaaatct aatagagcat aaaatgctcc tacgacttca
    23341 taagatatag cttgtaatgt atcaagtttt tgagtttcaa catacagatt atactcacaa
    23401 agtgttgcaa ttgtattatt atcaagccta gttaaagaaa tcgtatgtat atgattgttg
    23461 ataaattttg caatttcaat tcctgctttt gtttctccag acaaagaaat gataataaat
    23521 aaatcacttt tttgaaatga ttctctcata aattcaattt cacctatatc aaataagtca
    23581 ataacacatt tacctactga aagaaatatt ctctttattt cttgagctaa acttttctgt
    23641 tcattacctg tcccataaat ataaattgtt tctgcatcat atattatttt acaaatatct
    23701 tgaaatgata ttttttgtaa ttcattcatt aatagtcgat aattctcgca aacatcatga
    23761 aaattattat cattattaac aaattctagt tgattatttt taagtaaata ctttaaatca
    23821 gagaatgaat ctaatccaat ttttctacaa agccttaata aagtagctct agaaacatta
    23881 cattcatttg caattttttc acacgaaaaa ttacggtatt ctctttgaca aatcaatatt
    23941 tgtttaataa taattttatc attttcattt aaattatcat aattttggtt aactaacgat
    24001 tctaatctca ttttttccac tccctaaata ttattttaat atacattagt tgctttttct
    24061 agggaagagt tttaaataat aaggataaaa taatcatttg attttatttt tattaaaata
    24121 aaaataacct gctaaaagca ggcaccaaaa agtacttatc ctttcgaaca tgtactttag
    24181 atattgcttt cttcggaagc ttcatctctt catactctaa aatagagttt tttctaactc
    24241 aaaagtattt tccatggtag aaattacttt aattcatctg ccatttattt tttatcttat
    24301 ttaataatta ctactacttg aatatttact tctttaagta aatttcatag ttaacaaagc
    24361 taaaattatt attatagatg ttaaaataca aaattgtaag tattgttata aatatgctta
    24421 cagctacaaa tatacatgat atattaaaaa gaagctgtaa attattatca tacatacaag
    24481 caactacgcc tctattaatc catgttaatt tttattattt aatacaagga gttccatgag
    24541 acatacatat ccattcatat tttaaattat ccaattctct tatagatttc atcatctttt
    24601 tataatccca attccatgga ttaggataag gaactatatt tccattctta tttttgacta
    24661 aatcacctgc aaataataca tcttcaaaaa tcatgcagac atgccctgga gtatgaccag
    24721 gagtatggat aatttttata ttcccaatct gcatattctc tccataaggt tcaacatcct
    24781 taatgagcct tctggaaatt gcctttccaa taaacttctt aaacccaggt ctatccttct
    24841 ctcctataat aaaaggtata tcctctacat gagcccatac ttttgctcct gttttttctt
    24901 tcaataattt aatattagat atatggtcaa catcatgatg agttaataaa atatgcttaa
    24961 tatctgtaag tttaatgcca agtgttttta attctttaat tatatttttc cccataaaag
    25021 gaagacctgt gtctataaga ataatttctt tatcaaacac aataaaacaa tttgagccac
    25081 gagtactatc taacattaat acattttctt ttatcttcat aaataaattc cccttatttg
    25141 gtattttacc tatataatta attaataaag ttataaatag ttataatact tgatattaat
    25201 tttatttttt atta
//
LOCUS       DAMJYR010000049        24990 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.049, whole genome shotgun sequence.
ACCESSION   DAMJYR010000049 DAMJYR010000000
VERSION     DAMJYR010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 24990)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24990)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24990
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.049"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(24..632)
                     /locus_tag="PC471_001946"
     CDS             complement(24..632)
                     /locus_tag="PC471_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HDF2789076.1"
                     /translation="MEKDFFRKNGIDLAKSILGKYLIRKYENKVIVTKIIETEAYMGV
                     NDKGAHVYGNKKTDRTKPLYLDGGHIYVYLIYGMYNCLNLSANIENVPECVLIRGVEP
                     ITSLDEISMNRYNKAYTELSKYQVKNITNGPGKLCKALKIDRSLNSKSIMGEELYISD
                     FYYDDKGKKVFSKDELDIKTSKRINIDYAEEAKDFLWRFYIE"
     gene            complement(741..3479)
                     /locus_tag="PC471_001947"
     CDS             complement(741..3479)
                     /locus_tag="PC471_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HDF2789077.1"
                     /translation="MESKGTLSKNKVYILANGLFLVIALLNINLYTVSINMYRIILLW
                     IFGYSIVYISSFKTDEFINILKVGCLVLVFVSILGVVNLKIQKFQIVLLNLSIINMST
                     FIYLTISKVKKLRISPILIFYSAFVILDYFIRNIFSDINWNMIIILINICISMFNLLY
                     LLNKLDSNIEHYKLIKDLTYFVFFTTIICFVAISKINIVDSDRLIGVIYLLLCNHTYY
                     VYVYSLNNRVVYPYYELDSINKKLSKKSEQLGKINLAIEKDMIIQRTLKNYIDQRKEL
                     LRQALDTIPNVWIVTDYDFNISYTNNKFKDEFSKDMNNYYKILTFIKEDEKVAEKLKK
                     FDNDISIIDKLVELNDKIYLLSLSNNKDESNYLISLNDITNEIKIDEEIRNINKDYEN
                     IILNIPSPILVRSAEGGIKKNKVISINKKYEKSFKCVSSDLVDMTLEQYFETFNIDFF
                     DNREFKRLNLTQEKKAEIVSYNDTANCIVNFVMSDSEGEEHVEEVRVGDYWSDNKLFK
                     LLTFRDITKEINILRTVNEQKIIYEKLLDVIPEAIFLEDLSTSRVLYTNRAFRELFGI
                     SSDVLGVTTQKYRNILVKKYINNLNIGEREKSIHIVNENNHIKEVKMISRTLYFGQKR
                     SRVRIIKDLSVQRESERLKKALIKQRQYDQMKMEFYANISHELKTPLNNIYSSVQLIE
                     NLYKKGKIIDFQDILKEHIKITKQNMFRLLRLIDNIINISQVKSDIYKIKAVNFDIID
                     ITERIVTSISSYAKSKGIDLIFDTDEETVMVGLDPESIERIILNILSNAIKFTLEGGE
                     ILVGIYKKDETVEIIIKDTGVGIDKEKLKDIFNRFKQIENSGISNEFGSGIGLCLTKS
                     LIEIQNGKIYIDSKVGEGTNVKVIFPIKEVVEEVYDNSNYNDNIEKFEIEFFDIYK"
     gene            complement(3627..4418)
                     /locus_tag="PC471_001948"
     CDS             complement(3627..4418)
                     /locus_tag="PC471_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011987989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HDF2789078.1"
                     /translation="MKLKKLLSVALVSAIAISAVGCSNKEDKKILVGASSNPHAKILE
                     VAKPLLKEKGYDLEVKIFDDYVLPNTALDEGSLDANFFQHIPFLEETVKEKGYKLTYT
                     SKVHIEPMGFYSEKVKSLDELKDGAVIAVPNDATNGARALKLLAKNKLIEVKDGELIT
                     KKDITKNPKNIVIKEMNAEQLPTVLKDVDGAVINSNYALTANLNPTKDAIVIESSDSP
                     YVNIIACRENNKDSDKIKALSEAMNSKEVKKFIQDEYKGSIVPAF"
     gene            complement(4454..5110)
                     /locus_tag="PC471_001949"
     CDS             complement(4454..5110)
                     /locus_tag="PC471_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2789079.1"
                     /translation="MNSLIDFLTTLFPNALLQTLYMVIVPTIVATILGFILAIILVVT
                     KPDGLKPNSTINSALGFIVNIFRSFPFMILIVAMIPITRLIVGTSIGETAAIVPITIG
                     AAPFIARIIESSLNEVDKGLIEAAKSFGATKRQIVFKVMIKEAMPSIVSGITLSIISI
                     LGYTAMAGAVGAGGLGNIALIYGYQRFDTAVMVYTVIALIILVQIIQGVGNLAYKKLK
                     "
     gene            complement(5103..6068)
                     /locus_tag="PC471_001950"
     CDS             complement(5103..6068)
                     /locus_tag="PC471_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HDF2789080.1"
                     /translation="MISIKNVNKYYGKIQVLKDVSIEIESGEIFGIIGHSGAGKSTLL
                     RCINGLEEYQEGSVLVSDKEVKSLNEKQMRDLRKELGMIFQHFSLLERKTVFDNVALP
                     LECFGYSKAEIKKRVLELLEVVGISEKKNDKPRNLSGGQKQRVAIARALALNPQVLLC
                     DEATSALDPNTTKSILSLLEDINKKLGITIIVVTHQMEVIKQICGRVAIMENGEVLEV
                     GDTEEIFLRNTKGLRKLIGEESIILPKGTNIKILFPKDISNEAIITTMARELNIDVSI
                     IFGKLEQFKDDILGSLIINISDKSGEQVKQYLTSKGIRWEEMINE"
     regulatory      complement(6174..6280)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            complement(6570..7514)
                     /locus_tag="PC471_001951"
     CDS             complement(6570..7514)
                     /locus_tag="PC471_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YafY family transcriptional regulator"
                     /protein_id="HDF2789081.1"
                     /translation="MSRAERLIELMITINAKRSFTAGELAEEFSVSKRTILRDLQVLE
                     SIGFPLYSKVGAAGGYHVLKERILPPIAFSESEVKSIFFAYQSLEYYNDLPFEQETIS
                     VLKKFLNCIPNDIQYNIENIRRKFVFWTPDRHCSTPLLKELFNIVMNEFTIKIEYSSK
                     QKNSVRTIVPIGLYAMNGLWYCPAYCIKSESIKEFRVDRIVKILSIENLSSKKYKVLS
                     SIHDYLKNMEVGTDYHIKINLTDEGVKRCETEFLLARGLKILSKGGYIDMYIPKSTLN
                     WVAEYFLTFGKNATIIEPIELKHLIKSKVLELYNHHCI"
     gene            7625..8092
                     /locus_tag="PC471_001952"
     CDS             7625..8092
                     /locus_tag="PC471_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2789082.1"
                     /translation="MENDTIKADDILNYCLSNLDDVVLMDSWGERAIYYNPNGVLKRG
                     VYVLTIKEKDSNNDKGSLVSRPNVYRVNIGLKKETFIEMFGYIPKRPGVGQIVDMDFD
                     FTKLDTIMPHPIYSWMGWICVLSPTEKTFENFKTLIGESYNFAKQKFKKRKNK"
     gene            complement(8262..8861)
                     /locus_tag="PC471_001953"
     CDS             complement(8262..8861)
                     /locus_tag="PC471_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2789083.1"
                     /translation="MKLKRTLFVGLALVLAIGITACTKNNEKPSENTGKNPNESVNNN
                     EGYMNYYSTSYNDYIAGLNRYSIYDTPESINNQFKDKEYPGNEKYLSDVKAAYKDSRD
                     KIQSFVTSLKKDGKTEDTELKKMNDDLIAEGERLVKDIDKRIEKLDKVSDEDMKKGQN
                     DFIKLVHGTEDVGNDIGSGFRKMIKDMNDRLGITGNNKK"
     gene            9107..9703
                     /locus_tag="PC471_001954"
     CDS             9107..9703
                     /locus_tag="PC471_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3793 family protein"
                     /protein_id="HDF2789084.1"
                     /translation="MSCCNSQFCCKNKIESSYIKWLLEVLGPVFLGSKPAEIINISFT
                     DVNREEKINDIYKYLYKCKKIDFIVIDKEKKGLKILFVNKKALSEKLKCKKTVNFLKF
                     LGYKQNTNVNAYLEHLVDKLKSDVFPDEIGIFLGYPLKDVIGFMGYSNYEVSMIKYWK
                     VYGDTKQSEDTYSKFLLHRKKMRKLLDYISVDKIVSCF"
     gene            complement(9801..10760)
                     /locus_tag="PC471_001955"
     CDS             complement(9801..10760)
                     /locus_tag="PC471_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrtA/SsuA/CpmA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HDF2789085.1"
                     /translation="MTSLIMVLVGTVGCSTTNDKKDEPAKKEASLPKEINLTYVKSPL
                     NVPSILEKKDDLFGKEFKKDNIKVNFHELTTGPEQTQALAAGELDFLHALGGTSAIIA
                     ASNGVGLKITNIYSRSPKGYMLITNNDNIKSIKDLKGKKIVGPKGTILHQLLIAALAK
                     EGYTIDDVEYVNMDIPSAASALESKSADVAMLAGPVALKTINSGAKMIVNGEGLVSGI
                     IVTAVSDDFAEKYPELVKRFMKVHEETLKYMNENKDEVMDVVSKEVGLSLDETKEMYS
                     WYDFSSKITDKDIKELEDTQEFLMSNGMQQKKINIKDMLYNQN"
     gene            complement(10794..11525)
                     /locus_tag="PC471_001956"
     CDS             complement(10794..11525)
                     /locus_tag="PC471_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2789086.1"
                     /translation="MVRSGFLLENIYKKYLVDNKEHLVLDNISLNISSEEITVILGES
                     GCGKTTLLRILAGLENATSGNVYFFNNDKKCTPKVGMVFQESRLMPWLNVSENILLHT
                     EKDNRNKVDLDKYLKMMKLEKFKNSYPNELSGGMAHRVSIARALSFNPDILLMDEPFA
                     ALDYFTRRKMQKEVVNIHKNTKKGVVFVTHNIEEAMEIAKKIIVFSKNKRIKQFSVED
                     EYNRDLTKNYYINLKKEILRELGEF"
     gene            complement(11518..12288)
                     /locus_tag="PC471_001957"
     CDS             complement(11518..12288)
                     /locus_tag="PC471_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2789087.1"
                     /translation="MFEKIHKRLKGYYIFLIILILWKIVCVLGIWSSYILPPPEIVLD
                     TFIKMIYDGSIFLNVYASVRRILIGFLISSLMAIPLGVLFGANKKMYEYFKPLFEFMR
                     STPPLALVPMLILWFGIGEESKIIIIILASFFPIFLNTLKGIKNCDNKLIEVGKSFNL
                     SKKRIFYKIIIPNSMLDIVVGLSLGLGYSFRAIIGAELIAASSGLGYLISDGKDMSRT
                     DVVLVGIFVIGFLGILSDYLFSKIITKFSKGKQVDLNG"
     gene            complement(12373..13083)
                     /locus_tag="PC471_001958"
     CDS             complement(12373..13083)
                     /locus_tag="PC471_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIM24 family protein"
                     /protein_id="HDF2789088.1"
                     /translation="MFKFDVTNELMCIARGSGKFFAKKGAMVAFKGNFNFEKLLLGPS
                     NGGGLGRALLGHVQRSLTGEQMPIMVVEGEGEIYLAQNAYHVDVISIDPGDSIYVESE
                     NLLAFSEQLNYSVKLIGSGVISQKGLFTTHLVNKTGQNQDVAIITDGNPLILEGPCCV
                     DPDALVAWTGREPYPKVSKLSWKTFIGQTSGESYHMEFVEPGQIVIIQPSERLSGLKT
                     DSTPSDRMSSGGLRLGID"
     gene            complement(13263..13415)
                     /locus_tag="PC471_001959"
     CDS             complement(13263..13415)
                     /locus_tag="PC471_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HDF2789089.1"
                     /translation="MATFIIAAIVVVLMALAVMYMVKNSKKNGSSCGCGCSGCSSSKS
                     CHSAKR"
     gene            complement(13441..15597)
                     /gene="feoB"
                     /locus_tag="PC471_001960"
     CDS             complement(13441..15597)
                     /gene="feoB"
                     /locus_tag="PC471_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HDF2789090.1"
                     /translation="MSIKIGLIGNPNCGKTTMFNGLTGSSQYVGNWPGVTVEKKGGKL
                     KGNKDVEIVDLPGIYSLSPYTLEEVVTRNFMLDDKPDAVINIVDASNIERNLYLTTQV
                     LELGIPTVIALNMMDIVNKNGDKINIKELSEVIGCPVVEVTAVKGQGIMEAAEKAVEL
                     ASSNNKLNFKLPFVDESKDAIEKIEKIIEEKTPYIDVETRWLAIKLFERDENVIQKLD
                     ISKTILNSIEEITRNCEDELDDDSESIITANRYEFISSIISSIIKKNRKGKETVSDKI
                     DKIVTNRILALPIFALIMWGVYYIAVSSLGTIATDWTNDVLFGEIIQGNVSNFLASLN
                     VAEWLQGLVVDGLIGGVGAVLGFVPQIMLLFLLLSILEDCGYMSRVAFIMDRIFRKFG
                     LSGKSFIPMLISSGCGVPGVMSTRTIENDRDRKMTIMLTTFIPCGAKIPIIALFAGAL
                     FGGASWVAPSMYFLGIAMIIICGIILKKTSLFAGEPSPFVMELPQYHIPSAKGVLIHM
                     WDRGKAFIIKAGTIIFVACGVIWFLQSFNWSLQMVDAGDSILASLGNIVAPIFAPLGF
                     GNWQSSVATVTGLVAKENVVGTFGVLFGISDATEQDPTLLASVASMFTVASAFAFMAF
                     NMLCAPCFAAIGAIKREMGSWKWTWITLGFQTLTAYIIALLINQVGSLVLGTGGSIAG
                     AIISIFIAVAVVFVVLTYSNKNMKKEKMGKLSYMKN"
     gene            complement(15697..15918)
                     /locus_tag="PC471_001961"
     CDS             complement(15697..15918)
                     /locus_tag="PC471_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HDF2789091.1"
                     /translation="MNRLKDIKCGETVKVKKLEGEGATRRRIMDMGITRGVNIFIRKV
                     APLGDPIEVTVRDYELSIRKSDAEKIIVE"
     gene            complement(15931..16143)
                     /locus_tag="PC471_001962"
     CDS             complement(15931..16143)
                     /locus_tag="PC471_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HDF2789092.1"
                     /translation="MMPLILSNTGEEVVIKKISGSDKTRTFLNSIGFVVGTNVKIVSK
                     IDGNLIIDVKDTRVALDKKMASRIII"
     gene            complement(16464..19361)
                     /locus_tag="PC471_001963"
     CDS             complement(16464..19361)
                     /locus_tag="PC471_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HDF2789093.1"
                     /translation="MKAFKKHFLILSLIFLLIISCFNNILCFADESENSKKIRVGYCD
                     DYGIISSSKEHSYTGYGYDYLREISKYTRWEYEFVKGSWEECLDRLEKGEIDLLGPLQ
                     KNDERNKLFNFPKLSSGYEYSALYTKDSNNNMFYEDISSFKGMRVAVLRGNFHNTAFE
                     KYREENNFSVEYIYCNSIDELIESVNEKKADAFVCGSIINAKGMKIVSKFSVEPFYFA
                     TAKDKPNLVKELDYALKELKINDMYYELELYKKYFKREVNNSIAFTRQEMNFIKANPK
                     LTMVYDSEWSPVEYYDEESNSFKGISSDLMSIISKKCGIKFEYIKTKNYNESLDYIKS
                     GKATMLCGSINENDKVKRFNMKLTNPYINIPMIMVGKLDTNLNNDLNIALTSSYKSID
                     SYIEKSFENAKTTSYKSVESCLNAINEGKANLMILSSYQFDELLREGKSENLSVISVL
                     DTSYGMRIGVSNKTDPILVSILNKSIDKITEEELSDCIYSNTIDKPYKVPFGVIFKEY
                     SIQIISFVCILFLIAIKYIIYNKKKKEDYLRKIAYTDPLTGADSIDKFKINSNKLFAK
                     NNPEEYALFYIDVDKFKYINDMFGYDMGNDTLIHISNTIASELKEDEIFARVSADHFV
                     LLIKYKTDDDIKTRLNNIYNKVQILSNPKINYYKLILDCGIYKISKSDNDINTIMDRA
                     NTARKTIKGGHKNSFAFYDKEMHKKILKEKEIENSMVDALNNGEFIVYFQPKYSLSDY
                     QIIGAEALVRWDNPQKGLIPPIEFIPVFERNGFIVNIDFYVFEEVCKKIREWMDEGQK
                     VVPISVNLSRMHFVNSNFIEEFKLIVDKYKIPTRLIELELTETAVLDNIEGLLDTMNN
                     LKEKGFVISMDDFGTGYSSLNLLKELPVDILKLDRAFFTEKDESNNEKIVISNVIKMA
                     KELKMKVISEGVETISQVEFLKQIGCDMVQGYLFSKPMPVKEFEKIAFKKE"
     gene            complement(19688..20296)
                     /locus_tag="PC471_001964"
     CDS             complement(19688..20296)
                     /locus_tag="PC471_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HDF2789094.1"
                     /translation="MVDNLIKYIHKSDVAIVVLHEIYGINSFIEDVCQKYYKYGYDVF
                     CPELLGEKRVFPYSKVQEAYDFFINNVGFDIYKKVEKLINDLKSEYKYVFVAGYSVGA
                     TIAWRCSEDSFCDGIICCYGSRIRDYMDVIPKCPTLLVFAKHDSFDVDSISYQLSRKQ
                     NIQIEILDANHGFIDRYSNNYSDIQTKVFTIKEKSFLSKYLA"
     gene            complement(20556..21101)
                     /locus_tag="PC471_001965"
     CDS             complement(20556..21101)
                     /locus_tag="PC471_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH peroxidase"
                     /protein_id="HDF2789095.1"
                     /translation="MKKFVCTVCGYIHEGDAAPAQCPVCKVGADKFEEMKGEKNWADE
                     HRIGVAQGVDEEIIEGLRANFVGECTEVGMYLAMSRQADREGYPEVGEAYKRIALEEA
                     EHAAKFAELLGEVVVADTKENLRVRVDAEYGATDGKLKLAKKAKELGLDAIHDTVHEM
                     CKDEARHGKAFLGLLNRYFGK"
     gene            complement(21219..23042)
                     /locus_tag="PC471_001966"
     CDS             complement(21219..23042)
                     /locus_tag="PC471_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2789096.1"
                     /translation="MNLSTGNQKVSRLKKKEKPKDFKKTFKRIFMYFKKDKKITRFIF
                     LLVIIDSIISTVIPYTIGKVVDIIDIDTYSKSIFLKGIIILLSFYIIEFSVKLSKGIC
                     TARLSQSIVKEMRSNLFLKFKNLPITFFDKSSTGDIMSRVTNDVDNISTGISNSLVQL
                     ITGILNILMTFIMMFVLSPILTFCSVILIPIVMFVSNFIAKRTRVFFKQQQVELGKLN
                     AHIEEMISNINVVKSFNYERKSVNDFKEVNDRILNISLKSQIYTSLLMPIMNVISNIG
                     FATICIIGGILASKDIITIGVIASFLSYVRQFTRPLNELANLFNTLLSAIAGCERVFE
                     IIDEQEEQEVHNMFKSEINDNKDGNLNSHFVKGEIELKDVSFSYDSKKKILDNINLKI
                     KAGTSNAIIGETGSGKTTIINLITNFYHIDEGSILLDGKDIYSINRNYLRTCFGVVPQ
                     EGYIFNDTVMENIRYGNLEATDEEVIYASKIANAHKFIVKMREGYSTVLSDRGEDLSE
                     GQKQLISIARAVLKNAPILILDEATSNIDIATEAKIQEAINNLTYGKTSIIIAHRLST
                     ISNCDNIIVLENGRIVESGNHKELIKLKGHYYRNILLTQGK"
     gene            complement(23055..24830)
                     /locus_tag="PC471_001967"
     CDS             complement(23055..24830)
                     /locus_tag="PC471_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2789097.1"
                     /translation="MRYFKKYIKKYIVLFLTAVFFLTLEAFCDLAQPTIMAKIIDIGV
                     SNKNLDYILSTGAIMIGITFLGAIFAIIRSVISARVSQHFSYDLRQDVFEKINTYSLK
                     QIDKFGRASLITRITNDINQMQQFVNGMMRVFIKSPIMCIGSLIIAIKLNLKMSIIIL
                     IAVLIIFIIILLNMKIGYRLFKNVQESTDKVNSKLRQFLSGIKVVKLFCRYDYENQSF
                     EELNDELFKNSRKATTMMSFFRPTITIIINVSIIIILLIGRHLIYSGEIKIGIIVAYV
                     NYMTRILTSLIMISHVFNVFVRAKASYERISEVLVDENEEVIEREESLDVGEKDLKFV
                     NCDIIFENVDFSYNKNTDEKILKNVSFKIEEGQNIGVIGQTGSGKTSLVNLIQKLYII
                     DKGDIKIGGRSIYSISEESLRARIGVVFQNSSVFGKSILENVVMGNRDICKEDVVKSC
                     EIANANQFITKLENTYDEVLFQRGKNLSGGQRQRITIARAIAKKPQILILDDCFSAMD
                     INTETIIRKNIKEYISNLREKESKKCTVITISQKISSIIDCDKILVLDEGEVVGFATH
                     EDLLVSSPIYKKIFALQNLVSEDVN"
BASE COUNT         8226 a         4333 c         2445 g         9986 t
ORIGIN      
        1 acatatctta cctatataaa aacttattct atataaaaac gccataaaaa atcttttgct
       61 tcttctgcat aatctatatt tatacgttta ctagttttta tatctaactc atctttacta
      121 aatacttttt ttcctttatc atcataataa aaatcactta tatataattc ttcacccatt
      181 atactcttac tatttagact tctatctatt tttaaagctt tgcaaagttt tcctggtccg
      241 ttagtaatat tttttacttg atatttactc aactcagtgt aagctttgtt gtatctattc
      301 atagatatct catctaaaga agttataggc tctacccctc ttattaatac acactctggc
      361 acattttcta tatttgcaga taaattcaag caattataca taccataaat taaataaaca
      421 tatatatgtc caccatctaa atataatggc ttagttctat ctgtcttttt attgccatat
      481 acatgtgctc ctttatcatt tacacccatg tatgcttctg tctctatgat tttggtaaca
      541 attactttat tttcatattt ccttattaga tatttaccta atattgattt ggctaaatca
      601 attccatttt ttctaaaaaa atccttttcc aaaatttcac ctcataaaat ttatttaaaa
      661 cgcatcaaat acaactaaag taataaataa aattaactct tattaatgtg atatatataa
      721 acaataaatt tagacattta ttatttatat atatcaaaaa attctatctc aaacttttca
      781 atattatcat tgtaatttga attatcatat acttcttcaa caacctcttt aattgggaat
      841 ataactttta cattagttcc ttctcctacc ttactatcta tgtaaatctt cccattttga
      901 atttcaatca atgattttgt aaggcataaa cctataccac ttccaaattc atttgatata
      961 ccactgtttt caatttgttt aaatcgatta aatatatcct ttaatttttc tttgtctatg
     1021 cccactccag tatcttttat aattatctct actgtttcgt cttttttata tatacctaca
     1081 aggatttctc ctccttctaa agtaaatttt atagcatttg ataatatatt tagtattatt
     1141 ctttctatcg attctgggtc aagccctacc ataacagttt cttcatctgt atcaaaaatt
     1201 aaatctattc ctttagactt agcataacta ctaatagatg taactattct ttctgttatg
     1261 tcaataatat caaaattaac tgctttaatc ttatatatgt ctgacttaac ttgagaaata
     1321 tttattatat tatctattaa tctcaataat ctaaacatat tttgtttagt tatttttata
     1381 tgttctttta gtatatcctg aaaatcaatt atttttcctt ttttataaag gttttcaata
     1441 agttgaactg aactatatat attatttaaa ggtgtcttaa gttcatgaga tatattagca
     1501 taaaactcca tcttcatttg gtcatactgt ctttgtttta ttaatgcttt tttaagtctt
     1561 tcagactctc tttgcacact taaatcttta ataattctca ctctacttct tttctgtcca
     1621 aagtataatg ttcttgatat catttttact tctttaatgt gattattttc atttactata
     1681 tggatactct tctctctttc tcctatattt aaattgttta tgtatttttt tactaatata
     1741 tttctatatt tttgtgttgt aactcctaat acatcactag aaattccgaa caactctcta
     1801 aaggctctat ttgtatatag tactcttgat gtacttaaat cctcaagaaa aatagcctct
     1861 ggtataacat ccagtaattt ttcatatatt attttttgtt catttactgt tctaagaata
     1921 tttatctctt tagttatatc tctaaatgta agcaatttaa agagtttatt atctgaccaa
     1981 tagtctccaa ctctaacttc ttcaacatgt tcttcacctt ctgagtcaga catcacaaaa
     2041 ttaactatac agtttgctgt gtcattgtaa cttactattt cagctttctt ttcttgtgtt
     2101 agatttaatc ttttaaattc cctattgtca aagaaatcta tgttaaaagt ttcaaaatat
     2161 tgttctaatg tcatatcgac taaatcactt gaaacacatt taaaactctt ttcatatttt
     2221 ttatttatac ttattacctt attcttcttt atgccacctt cagcacttct tacaagtata
     2281 ggagatggaa tatttagtat aatgttttca tagtctttat ttatatttct tatttcttca
     2341 tctattttga tttcatttgt aatatcattt aaacttatta agtaattgct ttcgtcttta
     2401 ttattactta aacttaacaa ataaatttta tcatttaact ctaccagttt atcaattata
     2461 cttatatcgt tatcaaattt cttcaatttt tcagcaactt tttcatcttc ctttataaaa
     2521 gttaatattt tataataatt attcatatcc tttgaaaatt catctttaaa tttattattt
     2581 gtgtaactta tattaaaatc atagtctgtt actatccata catttggtat tgtatccaga
     2641 gcttgtctaa gtaattcttt tctctggtct atgtagtttt ttaatgttct ttgtataatc
     2701 atatcctttt ctattgctaa atttatcttt cctaattgct ctgacttttt acttaatttt
     2761 ttattgattg aatcaagttc ataatatgga tacactactc tattattaag actgtataca
     2821 tatacataat aagtatggtt acataatagt aaatatatta ctcctattaa tctatctgaa
     2881 tctactatat taatcttaga aattgctaca aaacatataa tagttgtaaa aaaaacaaaa
     2941 tatgttaaat cttttattaa cttatagtgc tcaatgttac tatcaagttt attcaacaaa
     3001 tatagtaaat taaacataga tatacaaata ttaattaata taataatcat attccagtta
     3061 atatcactaa aaatatttct tataaagtaa tctaatatca caaaagcact ataaaaaatt
     3121 agtattgggc ttattcttag tttttttact ttagaaatgg ttaagtatat aaaagtagac
     3181 atattaatta tgcttaagtt aagcaataca atttggaatt tttgaatttt taaatttact
     3241 actcctaaaa tacttacaaa aactaataca agacacccta ctttcaatat atttataaat
     3301 tcatctgttt taaagctact tatataaact atagaatatc caaatatcca aagtaaaatt
     3361 attctataca tatttataga aactgtatac aaatttatat ttaaaagtgc aataaccaaa
     3421 aataacccat tagctagtat atagacctta ttcttactta aagttccttt agattccata
     3481 ttccccacca cttcttcatt tttatatagt tttacctata aatatgttac cacaattctt
     3541 ccaaaaaatt tgtcactttc tagttataaa aaaaaatgag taaatatttt taattttact
     3601 cattttgtag attttgtata ttcagtttaa aaagctggaa ctatacttcc tttatattca
     3661 tcttgtataa attttttaac ttctttacta ttcatagcct ctgataaagc cttaatctta
     3721 tcactatctt tattattttc tctacaagct attatattta catatggaga atcacttgat
     3781 tctattacta ttgcatcctt agtaggattt aagttagctg ttaaagcata attagaattt
     3841 attactgctc cgtcaacatc ttttaatact gttggtaatt gctctgcatt catttctttt
     3901 attactatat ttttaggatt tttagttata tcttttttag ttataagctc tccatctttt
     3961 acttctatca atttattttt tgctaataat ttaagtgctc tagctccatt agttgcatca
     4021 tttggcacag ctataacagc tccatctttt aattcatcta atgacttaac tttttctgaa
     4081 tagaatccca ttggctctat atgaacttta gatgtataag taagtttata tcctttctcc
     4141 ttcacagttt cctctaaaaa tggaatatgt tgaaagaagt tagcatccaa agacccttca
     4201 tctaaagctg tatttggaag tacataatca tcaaatattt ttacttctaa atcatatcct
     4261 ttttctttta acaaaggttt tgcaacttcc aatatctttg catgtggatt tgatgaagct
     4321 cctactagta tttttttatc ctctttgttt gaacaaccta ctgctgatat tgctattgct
     4381 gacaccaatg ctactgataa taatttcttt aatttcattt ttactccccc tctttaaaat
     4441 gtttataatt aatttatttt agtttcttat aagccagatt tcctactccc tggattatct
     4501 gaactaatat tataagtgct attacagtat ataccattac tgctgtatca aatctttgat
     4561 atccataaat aagtgctata ttacctaatc ctccagctcc tactgcacct gccatagctg
     4621 tataaccaag tatagatatt attgataatg taattccaga tactattgaa ggcatagctt
     4681 cctttatcat aactttaaat actatttgtc tttttgtagc tccaaaagat tttgctgctt
     4741 ctatcaatcc tttatcaact tcatttaatg agctttctat aattctagcg ataaacggtg
     4801 ctgccccaat agttattggc actattgcag cagtttcccc tatacttgtt ccaacgatta
     4861 atctagttat tggaatcatt gcaactataa gtatcataaa tgggaaactt ctaaatatat
     4921 ttactataaa tcctaatgca ctatttattg tgctatttgg ttttaatcca tctggttttg
     4981 taactactaa tatgattgct aatataaatc ctaatattgt tgccactata gttggtacta
     5041 taaccatata caaagtttgt aacaatgcat ttggaaatag tgttgttaaa aagtcaatta
     5101 aactattcat ttatcatttc ctcccatctt atgcccttac tagttagata ttgttttact
     5161 tgctctccac ttttatctga aatgtttata ataagtgaac ctaaaatatc atctttaaat
     5221 tgttctaatt tcccaaatat tatagacaca tctatattta gttctcttgc catagtcgta
     5281 ataatagctt cattagaaat atctttaggg aatagtatct ttatatttgt tccttttggc
     5341 aatattatac tttcttcacc tattaatttt ctaagtcctt tagtatttct taagaatatt
     5401 tcttctgtat ctcctacctc taatacttct ccattttcca ttattgctac tcttccacaa
     5461 atctgcttta taacttccat ttgatgagtt acaactatta ttgttattcc caatttttta
     5521 tttatatctt caagcaatga aagaattgac tttgtagtat ttgggtctaa tgctgaagtc
     5581 gcttcatcac aaagaagtac ttgtggattt agtgctaaag ctctagcaat tgcaaccctt
     5641 tgtttttgtc cgccacttag atttctgggt ttatcatttt tcttttcaga tattccaact
     5701 acttctaata actctaaaac tcttttttta atttctgcct ttgagtatcc aaaacattct
     5761 aatggcaatg ctacattatc aaatacagtt tttctttcaa gcaatgaaaa gtgttgaaaa
     5821 atcattccta actcttttct taaatctctc atttgctttt cattcaatga ctttacttct
     5881 ttatcagaaa caagtacact gccttcttgg tattcttcta atccgtttat acatctcagt
     5941 agtgttgatt tccctgcacc actatgacct attattccaa aaatttctcc agattctatt
     6001 tcaatactta catctttaag cacttgaatc tttccataat acttattaac attttttatg
     6061 ctaatcataa aatttcctcc catatattct gctcagtttt tatgtttatc ctctcttatg
     6121 tattacaaca aaataaaagc ccgagtataa actcaggctt agaaagctat ttatactcat
     6181 ctatcaacta tattagttgc aggatttagc accaatgtat ataaaatata ctggttgccg
     6241 ggtttcaaag ggccagtccc tccaccactc ttgataagac gttatttaat ttaattctat
     6301 tatcatttgt tgctatgtta tatatgatat attcattaat agtatttgtc aataaattta
     6361 tttaaatttt ttatttttta ttatcgttaa ttaactattt ttaattagtt ttctagctcc
     6421 taacacttta tattttaatg tctatataac tatagaacat cattataaga aataaattaa
     6481 ataacatcaa tctatctaaa tcatctgtaa agtaaattaa gaaaattaac attttatcca
     6541 aactctatcc tactattaaa taaaataatt taaatacaat gatgattata tagctctagt
     6601 actttagact ttatcaaatg tttaagttct ataggttcta ttattgtagc atttttccca
     6661 aaagttaaaa aatattcagc cacccagtta agtgtagatt ttggtatata catatctatg
     6721 tatccacctt tagacaagat tttcaaccct cttgccaaca aaaattctgt ttcacaacgt
     6781 tttactccct catctgtcaa attaatttta atatgataat ctgtacctac ttccatattc
     6841 ttaagataat catgtataga agataaaacc ttatatttct tacttgataa gttttctata
     6901 ctcaaaatct tcacaattct gtctacacga aattccttaa tagattctga ttttatacag
     6961 taagctggac aataccaaag tccattcatg gcatacaaac caataggaac aatggtacgt
     7021 acactatttt tttgctttga tgaatattca attttaattg taaattcatt cattactata
     7081 ttaaacaatt ctttcaataa tggtgttgaa cagtgcctat ctggtgtcca aaaaacaaat
     7141 ttacgcctta tattctcaat attatattga atatcatttg gtatacaatt taaaaatttt
     7201 tttaaaacag aaatagtttc ttgctcaaat ggcaaatcat tataatattc tagtgactga
     7261 tatgcaaaga atatagattt tacctcactt tctgaaaatg caatcggtgg aagtatacgc
     7321 tctttaagta catgatatcc accagccgct ccaacttttg aatataatgg aaatccaata
     7381 ctctccagca cctgtaaatc tcttagtata gtacgttttg atacagaaaa ttcttctgct
     7441 aattctcctg ctgtaaaact tctttttgca tttattgtta tcatcagttc aattaaacgc
     7501 tcagctcttg acatatttat actcctaatt taaatatagt gacaataatt gtcactatta
     7561 tatgataaga tattcttgca tctaaagcaa taataatagt aaagaaagaa gggattgata
     7621 ctttatggaa aatgatacta ttaaggctga tgatattctc aattattgtc tatcaaactt
     7681 agatgatgtt gtactaatgg atagttgggg ggaacgagca atttactaca atcctaatgg
     7741 tgttttaaag cgaggggtat atgttcttac cattaaggaa aaagacagta ataatgataa
     7801 aggttcgtta gttagtcgtc caaatgtata ccgtgtgaat ataggattaa aaaaagaaac
     7861 ttttattgaa atgtttggat atattccaaa gcgtccaggt gtaggtcaaa tagttgatat
     7921 ggattttgat tttacaaaat tggacacaat catgccacat cctatctact catggatggg
     7981 atggatatgt gtcctaagcc ctactgaaaa gacatttgag aactttaaaa ctttaatagg
     8041 agaatcttac aattttgcaa aacaaaaatt taaaaagaga aaaaacaagt aaaaacgtgt
     8101 gaaaataacc tctaaataaa ttataataaa aatattaagg ttacaagata aaaattaata
     8161 tcttaataac atgcaaaaaa gcgaagccaa aaaatgtatt atttataagg cttcgctata
     8221 tctcaaataa tttatttcaa atttcaaaac tatttattct attatttctt attatttcct
     8281 gttattccta atctatcatt catatctttt atcatttttc taaaaccact acctatatca
     8341 tttccaacat cttcagttcc atgaactaat tttataaagt cattttgacc ttttttcata
     8401 tcttcatcag ataccttatc taatttttct attctcttat ctatatcttt tactaatctt
     8461 tccccttcag cgattaggtc atcattcatt ttctttaact ctgtatcttc tgttttacca
     8521 tcttttttaa gagatgttac aaatgattgt attttatctc tactatcttt atatgcagcc
     8581 tttacatcac ttagatattt ttcatttcct ggatattctt tatctttaaa ctgattattt
     8641 atactttctg gagtatcata aatagaatat ctatttagac ctgctatata atcattatag
     8701 cttgttgaat aataattcat atatccttca ttattattta cagattcatt aggatttttt
     8761 cctgtatttt cacttggttt ttcattattt ttagtacatg cagttatacc tatagctaat
     8821 actagtgcta atcctacaaa taaagttctt tttaatttca tttttattac cctcctagat
     8881 gttttatact ataaaaaatt ttaataattt tctttcttaa tttagtattt ctatattttt
     8941 atattttatt tactaaaatt aaacaagtta taatttattt ttttggaaat cataatgaat
     9001 aaataattaa ataaaaaaat tataatttta tcattgatat tgattatcat tcatagtata
     9061 atctaattac ttgatattaa ttttcatttg aaggaggtta tcttttatgt cttgttgtaa
     9121 ttcacaattt tgttgtaaaa ataaaattga atcatcatat ataaaatggc ttttagaagt
     9181 tttaggtcca gtctttttag gctctaaacc tgctgaaata ataaatattt cgtttactga
     9241 tgtaaataga gaggaaaaaa ttaatgatat ttataaatat ctatataagt gtaagaaaat
     9301 agattttata gttattgata aagaaaaaaa gggattaaaa atcttatttg taaataaaaa
     9361 agcattatca gaaaaattaa aatgtaaaaa aacagtaaac tttttaaagt ttttaggata
     9421 caaacaaaat acgaatgtta atgcttattt agagcattta gtagataaac ttaagagtga
     9481 tgtttttcct gatgaaatag gtattttttt aggatatcct cttaaagatg tgattggatt
     9541 tatgggatat agtaattatg aagtttctat gattaaatat tggaaagttt atggtgacac
     9601 aaaacaatcc gaggacacat attcgaagtt tttacttcac cgtaagaaaa tgagaaaatt
     9661 actagactac ataagtgtag ataaaatagt aagctgtttt taattgtgtt tttgtataat
     9721 aataaaaata caccctcttt taccatacag tttccataat aaataagggt gtattttcca
     9781 ctttaatatt ttaaagtatt ctaattttga ttgtacaaca tatctttaat atttattttc
     9841 ttttgttgca ttccattaga catcaaaaat tcctgtgtat cttctaattc ttttatatcc
     9901 ttgtccgtta ttttagaact aaagtcatac caagaataca tttcttttgt ttcatctaaa
     9961 gataaaccaa cttctttaga tacaacatcc atcacttcat ccttgttttc attcatatat
    10021 tttaaagttt cttcatgaac cttcataaat ctttttacta attcaggata tttttcagca
    10081 aagtcatcac ttacagcagt aactattatt ccacttacta atccttctcc atttacaatc
    10141 attttggcac ctgaatttat cgttttcaat gccactggac ctgcaagcat tgctacatca
    10201 gcacttttac tttctaatgc agaagcagca ctaggaatat ccatatttac atattcaaca
    10261 tcatctatag tatagccttc tttagcaagt gcagcaatca acagttgatg aagtatagtc
    10321 ccttttggac ctacaatttt tttacctttt aagtctttta tagatttaat attatcatta
    10381 ttagttataa gcatatatcc ttttggtgaa cggctataaa tattcgtaat tttaagacca
    10441 acaccatttg atgcagctat tattgcagac gttcctccta atgcatgtaa aaagtctagc
    10501 tcacctgctg ctaatgcttg agtttgctct ggtcctgttg tcaattcatg gaaattgact
    10561 tttatattgt cctttttaaa ttcctttcca aataaatcat cctttttttc taatatacta
    10621 ggaacattaa gtggtgattt tacataagtt aaatttattt ccttaggtaa actagcctct
    10681 ttttttgcag gttcatcttt cttatcattt gttgttgaac atcctacagt cccaaccaac
    10741 accattatta aagatgtcat tactgctaaa atctttttgt ttcttttaat caattaaaac
    10801 tcccccaatt cccttaatat ttcttttttc aagtttatat aatagttttt agttaaatct
    10861 ctattatatt catcttcgac actaaattgt ttaatccttt tatttttact aaaaacaatt
    10921 attttctttg ctatttccat agcttcttca atattgtgag ttacaaatac tacacctttt
    10981 ttagtatttt tatggatatt tacaacttct ttttgcattt ttcttcttgt gaagtaatct
    11041 agtgctgcaa atggttcgtc catcagcaaa atatcgggat tgaaggaaag tgctcttgct
    11101 atacttactc tgtgagccat tcctcctgat aattcatttg gatatgagtt tttaaacttt
    11161 tcaagcttca tcatttttaa atacttgtct aaatcaactt tatttctatt atctttttca
    11221 gtatgtaaaa gtatattttc acttacattc agccatggca taagtctact ttcttgaaat
    11281 accattccta cttttggagt acacttttta tcattattaa aaaaatatac gtttccacta
    11341 gtagcatttt caagccctgc taaaattcta agtagcgttg ttttcccaca tccactttca
    11401 cctaatataa cagttatctc ttcactagat atatttaaag atatattatc aagaactaaa
    11461 tgctctttat tatcaactaa atatttctta tatatattct ctagcaaaaa tccacttcta
    11521 accatttaaa tcaacctgct tccctttgct aaattttgtt attatctttg aaaatagata
    11581 atctgaaagt atacctaaaa atccaataac aaaaattcct accaatacaa catcagttct
    11641 agacatatct tttccatcag aaatcaaata tcctagtcct gaagaagctg caataagctc
    11701 tgctccaatt atagccctaa aactataccc aagccctaaa ctaagtccaa caacaatatc
    11761 cagcatagaa ttagggatta ttattttata aaaaattctc tttttagata aattaaaaga
    11821 tttacctact tctattaatt tattgtcaca attttttata cctttcaatg tatttaaaaa
    11881 tattggaaaa aaggatgcca aaataataat tataatttta gattcttcac ctataccaaa
    11941 ccataaaata agcattggca ccaaagctag tggaggtgta cttcgcataa attcaaatag
    12001 tggtttgaaa tattcataca tttttttatt tgctccaaat aaaactccaa gtggtatagc
    12061 catcaatgaa cttattaaaa aacctattaa tatccttctt acactggcat aaacatttaa
    12121 gaaaatagaa ccgtcatata tcatttttat aaaagtatct aagactattt caggaggagg
    12181 cagaatatag ctactccata tacccaaaac acatactatc ttccataaaa ttaaaattat
    12241 taaaaaaata taatatcctt taagcctttt atgaattttc tcaaacatat aaaactcctt
    12301 ttaacatatc tatccctgct acccttttta ttttttaata tcttaaataa taattaagct
    12361 atatcataag atttaatcta tgccaagtct taatccacct gaactcattc tatcagaagg
    12421 tgtagaatct gtttttaagc cagacaatct ttcagatggt tgtataatga ctatttgtcc
    12481 aggttcaaca aattccatat gataagattc tcctgaagtc tgaccaataa aagtcttcca
    12541 tgatagtttt gatactttag gatatggttc tcttccagtc caagctacta atgcatctgg
    12601 gtcaacacaa caaggtcctt caagaattag aggattacca tctgttatta tagcaacatc
    12661 ttgattttga cctgttttat ttactaaatg tgtagtaaaa agaccttttt gagatattac
    12721 ccctgaacca atcaatttta cactgtaatt taattgttca gaaaatgcca ataagttttc
    12781 actctcaaca taaatggaat cacctgggtc aattgatatt acatctacat ggtaagcatt
    12841 ttgagctagg taaatttcac cttcaccttc aaccaccatt attggcattt gttctcctgt
    12901 caatgacctc tgaacatgac ctaataatgc tcttcccaat ccaccgccat tacttggacc
    12961 taacagcaat ttttcaaaat taaaatttcc tttaaaggct accatagcac ctttttttgc
    13021 aaaaaattta ccacttcccc ttgctataca cataagctcg tttgtaacat caaatttaaa
    13081 cataatattc acttatattg cttaatttag caataatccc tcctaatcat atatttatat
    13141 ataattatac agtaaaatta cagcatagtc taacctactt caaatatgaa atttgttatt
    13201 tatacaaaaa ataagaacct gataataaag caatttaact ttttatcagg ctcttatcct
    13261 atttatctct tagcgctatg acaactctta ctacttgagc atccagaaca accacatcca
    13321 caactgctac cattcttttt tgaattttta accatataca ttacagctag tgccattaat
    13381 actactacaa tagctgctat aataaatgta gccatattca tcactcctta tttatatgaa
    13441 ttaatttttc atataactta atttacccat tttttctttt ttcatatttt tatttgaata
    13501 agttaaaact acaaagacta ctgctactgc tataaatata gatattattg ccccagcaat
    13561 acttcctcca gtacctaaaa ctaaacttcc aacttgattt ataagaagag caattatata
    13621 cgctgttaaa gtttggaatc ctaatgtaat ccatgtccat ttccatgaac ccatttctct
    13681 ttttatggct ccaatagctg caaaacaagg tgcacaaagc atattaaatg ccataaatgc
    13741 aaacgcacta gctactgtaa acatacttgc aactgaagca agaagtgttg ggtcttgctc
    13801 tgttgcatct gatataccaa ataaaactcc aaatgttcca actacatttt ccttagctac
    13861 tagacctgta actgttgcta ctgatgattg ccaatttcca aatccaagag gagcaaatat
    13921 tggtgcaact atatttccta agctagctaa tatactatct ccagcatcta ccatttgtaa
    13981 tgaccaatta aatgattgta agaaccagat tactccacaa gcaacaaata taattgttcc
    14041 tgctttgatt ataaatgctt ttcctctatc ccacatatgt attaaaacac cttttgcact
    14101 tggaatatga tattgaggta attccataac aaatggagat ggttctcctg caaataaact
    14161 agttttcttt aatataattc cacaaatgat tatcattgca atacctaaga aatacattga
    14221 tggagctacc catgaagcac ctccaaataa tgcccctgca aataatgcta ttattggaat
    14281 cttagctcca catggaatga atgtagttaa cataatagtc atttttctat ctctatcatt
    14341 ctctatagta cgagttgaca taactcctgg aactccacaa cctgaactta taagcattgg
    14401 tataaatgat tttcctgaaa gaccaaactt acggaaaatt ctatccatta taaaagctac
    14461 acgtgacatg tacccacaat cttctaatat tgataataat aagaataaaa gcatgatttg
    14521 tggtacaaat cctagtactg ctccaactcc accaattaga ccatcaacta caagtccttg
    14581 taaccactct gctacattta aagaagctag aaaattactt acattgcctt gaataatttc
    14641 tccaaacagt acatcatttg tccaatcagt tgctatagta cccaatgaac ttactgctat
    14701 atagtaaact ccccacatta taagtgcaaa aataggtaat gctagtatac gatttgttac
    14761 aatcttatct atcttatctg atacagtttc cttacctttt cgattcttct ttattatact
    14821 tgaaattatt gaactaataa attcataacg attggctgtt attatacttt cactatcatc
    14881 atccaattca tcttcacaat ttctagttat ttcttcaata ctattcaata ttgttttaga
    14941 aatatcaagt ttttgtataa cattttcatc tctctcaaat agctttatag ctaaccagcg
    15001 tgtttctaca tctatatatg gagtcttttc ttctattatt ttttcaattt tttctattgc
    15061 atccttactt tcatcaacaa agggtaattt gaaatttaat ttattgttac tacttgcaag
    15121 ttcaactgcc ttttcagcag cctccataat tccttgacct ttaactgctg ttacttctac
    15181 cactgggcaa cctataactt cagatagttc tttaatattt atcttatcac cattcttatt
    15241 tacaatatcc atcatattaa gagctataac ggtcggtata cctagctcta aaacttgagt
    15301 tgtaagatat aaatttctct ctatatttga tgcatctaca atatttatta ctgcatccgg
    15361 cttatcatct agcataaaat tacgtgtaac tacttcttct aatgtatatg gagataaaga
    15421 ataaatacct ggtaaatcta ctatttccac atctttattt ccttttaatt ttccaccttt
    15481 tttttcgact gttactcctg gccagttacc aacatattgt gatgagcctg taagcccatt
    15541 gaacattgtt gtcttaccac agttaggatt tcctatgaga cctattttta ttgacatttc
    15601 cattccctcc attattattt aaatacatca ctgtttgtga tacagaaaat cattctcatt
    15661 atataattac attaacccta ctctaattaa tctttattat tccacaataa ttttttctgc
    15721 atctgactta cgaattgata gttcataatc tctgactgtt acctcaattg ggtcaccaag
    15781 aggagctact tttcttataa atatattaac acctcttgta atccccatat ccattatacg
    15841 tcttcgtgta gctccttctc cctctaactt tttgactttt acagtctctc cacacttaat
    15901 atccttcagt ctgttcatgt cattacctcc ctatattatt attctacttg ccattttttt
    15961 atctaatgca actctagtat cttttacgtc tattattaag ttaccatcta tcttagaaac
    16021 tattttaaca tttgtaccca ctacaaaacc tatactattt aaaaaagtcc tcgtcttatc
    16081 acttcccgaa attttcttta taactacttc ttcacctgta tttgataata tcaatggcat
    16141 catataatat ccctcctctt aaagtaattt aaatctacaa gcaatcgtta tcttatatat
    16201 aaattacaat accttaaatc ttaatctatt aaaataacat cctatttttt ccttcttttt
    16261 acaattccta attttgattt aagtctatca caatagtata ttttttgcaa gtgattattg
    16321 tattcttttg attttttttc tcatttgatt tattttatta ataaaatcgt aaaaattaat
    16381 acaaaaataa agatgctcta aaatagtata ctcaactaat attaaagcac ctttattata
    16441 tatgtcatta tttacacatt gctttattct tttttaaaag cgattttttc aaactctttt
    16501 acaggcattg gcttagaaaa caaatatcct tgtaccatat cacacccaat ttgctttaga
    16561 aattctactt gcgaaatagt ctctacacct tctgatatta ctttcatttt cagttccttt
    16621 gccattttaa tgacattaga tataacaatt ttttcattat tactctcatc tttttctgta
    16681 aaaaatgctc tatctagttt taatatgtca acaggcaatt cttttaaaag attaagagag
    16741 gaatatcctg taccaaaatc gtccatagag attacaaatc ccttttcttt caaattgttc
    16801 attgtatcaa gtaatccttc tatattatct aatactgctg tttctgttag ctctaattct
    16861 ataagtcttg tcggaatttt gtatttatct acaattaatt taaactcctc tataaaatta
    16921 ctatttacaa aatgcattcg agacaagttt acagatatag gtactacttt ttgtccttca
    16981 tccatccatt ctctaatctt tttacacact tcctcaaata catagaaatc aatatttaca
    17041 ataaaaccat tcctctcaaa tacaggaata aattctattg gtggtattag acctttttgt
    17101 ggattatccc aacgaacaag agcctctgcc cctataattt gataatctga aaggctatat
    17161 ttaggttgaa aataaacaat aaattctcca ttattaagag catctaccat tgaattttca
    17221 atttctttct cttttaatat ttttttgtgc atttccttat cgtaaaaagc aaatgaattt
    17281 ttatgaccac cttttatagt ttttcttgca gtattagctc tatccataat agtatttatg
    17341 tcattatcac ttttacttat tttatatata ccacaatcta aaatcaattt ataatagttt
    17401 attttaggat tactaagtat ttgaacctta ttgtaaatat tgtttaacct tgttttaata
    17461 tcatcatctg tcttatactt aataagaaga acaaaatggt cagcagaaac tcttgcaaaa
    17521 atttcatctt cctttaattc acttgcaata gtatttgata tatgtatcaa agtatcattt
    17581 cccatatcat atccaaacat atcatttata tatttaaatt tgtcaacatc tatataaaac
    17641 aacgcatatt cttctggatt attcttagca aacagtttat tagaatttat tttaaattta
    17701 tctatactat ctgctcctgt caatgggtct gtatatgcta tcttccttag ataatcctct
    17761 ttcttctttt tattataaat tatatattta atagcaatta agaataaaat acatacaaaa
    17821 gaaataattt gtattgaata ttctttaaaa atcacaccaa atggtacttt ataaggtttg
    17881 tcaatagtat tagaatatat acaatcactt aattcttctt cagtaatttt gtctattgat
    17941 ttatttaaaa tagacactaa tatagggtca gttttatttg atacccctat acgcatccca
    18001 tagcttgtat ctaaaacaga tataacagat aaattctcac tctttccttc tcttaaaagt
    18061 tcatcaaact gataagaact aagtatcatt aagtttgctt taccttcatt tatagcgttt
    18121 agacaacttt ctacactctt ataagatgtt gtcttagcat tttcaaaact cttttctata
    18181 taagaatcaa tagacttata actactagtt aaagcaatat ttaaatcatt attgagattg
    18241 gtatccaatt ttcctaccat aatcattgga atatttatgt atggatttgt caatttcata
    18301 ttaaatcgtt ttaccttatc attttcattg attgagccac atagcatagt tgccttacca
    18361 ctttttatgt aatcaagact ctcattataa ttttttgttt taatatattc aaattttata
    18421 ccacattttt ttgatataat tgacattaaa tcggaactta tacctttaaa tgaattgctt
    18481 tcttcatcat aatactctac aggagaccac tctgaatcat ataccatagt aagttttggg
    18541 tttgctttaa taaaattcat ttcttgccga gtaaatgcta tggaattatt gacctctctt
    18601 ttgaaatatt ttttatataa ttccaactca taatacatat catttatttt taattctttt
    18661 aaagcataat ctaattcttt tactaaatta ggtttatctt ttgcagtagc aaaataaaaa
    18721 ggttctactg aaaatttgct aactattttc attcctttag catttataat acttccacaa
    18781 acaaatgcat ctgccttttt ttcattgacg gactcaatta attcatcaat agaattacag
    18841 tatatatatt caactgaaaa attattttct tctctatact tctcaaatgc agtattatga
    18901 aagtttcctc taagtacagc tactctcatt cccttaaagg aacttatatc ttcataaaac
    18961 atattattgt tactatcctt tgtatatagt gcactatatt catatcctga acttagttta
    19021 ggaaaattaa atagtttatt tctttcatca ttcttctgta aaggtccgag taagtctatt
    19081 tcaccctttt ctagtctgtc taaacattct tcccaagacc cttttacaaa ttcatattcc
    19141 catcttgtat attttgagat ttctcttaga taatcataac catatcctgt ataagaatgc
    19201 tcttttgatg aggatattat tccgtagtca tcacaatatc caactcttat tttttttgaa
    19261 ttttcagact catcagcaaa gcatagtata ttgttaaaac atgatataat aagtaaaaat
    19321 atcaaagaaa gtatcaagaa gtgcttttta aatgctttca tatttaaaaa cctccaattt
    19381 gccaacttac tttaacttat ttcgttttct agttgtaact acttttattg taatggtaaa
    19441 tatatcaaat tgcaatattc tcatataata ttcttattca actataatga atttattatt
    19501 atctatttaa atacactatc taatattagt ttcttataag tcataaaagc acaactaaca
    19561 ctataattct agtgattaat tttatttcaa aaaatataat aacactacta atattttaaa
    19621 tatcaatagt gtcataaaaa cttttcagat taattaaatc tttctatata ttttataata
    19681 atctaaatca tgctaaatat ttagacagaa aacttttttc tttaatagta aatacttttg
    19741 tttgaatatc agaataatta tttgaatatc tatctataaa accatgattt gcatctaaaa
    19801 tctcaatttg gatattttgt ttcctagaaa gttggtaaga aatagaatca acatcaaatg
    19861 aatcgtgctt tgcaaatact aataaggtag gacattttgg aattacatcc atgtaatctc
    19921 taatccgaga gccataacaa caaattatgc catcacaaaa agaatcttca ctgcatctcc
    19981 atgcaatagt tgctcccacg ctatacccag caacaaatac atatttatac tctgatttta
    20041 aatcatttat aagcttttca acttttttat aaatatcaaa accaacatta tttataaaaa
    20101 aatcatatgc ctcttggact tttgaatatg gaaaaactct tttttctccc agaagttcag
    20161 gacaaaatac atcatatcca tatttataat acttttgaca aacatcttct ataaagctat
    20221 taatcccata gatttcatgc aaaacaacaa tagctacatc acttttatgt atatacttta
    20281 tcagattatc caccaccttt acaaaacagc aaatttttta tacttagttt aatatattct
    20341 tctttacatg tcaatttact tttctaaaat caaatttatt tttggattaa tcatttattt
    20401 ttaaaagttg tgcattatta ctattaaatt attctctatt attaaaaata taatcgttag
    20461 aaataaaaaa taaagtacaa ataattaaaa aatatctcac tcataacttt aaaaattata
    20521 agtgagatac tcctaaaatc tactttatat taaatttatt ttccaaaata tctatttaat
    20581 aatcctaaaa atgctttacc atgtctagct tcatctttac acatttcatg tactgtatca
    20641 tgtatagcat ctaatcctaa ttcttttgct ttcttagcta attttaattt tccatcagtt
    20701 gcaccatact cagcatcaac tctaactctt aagttttctt ttgtatctgc aactacaact
    20761 tctccaagaa gttctgcaaa tttagcagca tgttcagctt cttcaagagc tattctctta
    20821 taagcttctc ctacttctgg ataaccttct ctatcagctt gtctactcat tgctaaatac
    20881 attcctactt ctgtacactc accaacaaag ttagctctta atccttcgat tatttcttca
    20941 tctacacctt gagctactcc tattctgtgc tcatcagccc aatttttttc gcctttcatt
    21001 tcttcaaatt tatcagctcc aactttacat actggacatt gtgctggtgc agcatctcct
    21061 tcatgtatat atccacatac tgtacaaaca aatttcttca taattacttt cctcctaaat
    21121 tttatatatc aaattaaatt ttttatttat taagttagtc tatatatacc cgcaccatat
    21181 acctttaaac aattttttta aaagtttttt ctaaatactt attttccctg tgtcaataat
    21241 atgtttctat aataatgtcc ctttaatttt atcaattctt tatgatttcc tgattccaca
    21301 attctaccat tttctaaaac tattatatta tcacaatttg atatggtact taatctatga
    21361 gctataatta tacttgtctt tccataagtt aaattattta ttgcttcttg aattttagct
    21421 tctgttgcta tatctatgtt acttgtagcc tcatccaaaa taagtattgg agcatttttt
    21481 aatactgctc tagcaatact tattaattgt ttttgacctt cacttaaatc ttcaccccta
    21541 tccgataata cagtactata tccttcacgc attttaacta taaatttatg tgcatttgct
    21601 attttactag catatataac ttcttcatca gtagcctcca gatttccata ccttatattt
    21661 tccataactg tatcattgaa tatataacct tcttgtggaa ctactccaaa acaagttctc
    21721 aaatagtttc tgttaataga gtatatatcc ttaccatcta aaagtatact tccttcatct
    21781 atatgataaa aatttgtaat tagatttata atagttgttt ttccacttcc cgtctctcct
    21841 atgatggcat ttgatgtgcc agctttgatt tttaggttta tattatctaa aattttcttt
    21901 tttgaatcat aagaaaaact tacatcttta agctcaattt ctccctttac aaaatgagag
    21961 tttaaattac catctttatt atcgttaatc tcacttttaa acatgttatg cacttcttgc
    22021 tcttcttgtt catctataat ttcaaaaact ctttcacatc cagctatggc tgaaagtaat
    22081 gtattaaata gatttgctaa ttcatttaaa ggtcttgtaa attgtcttac ataacttaaa
    22141 aaactagcta taacccctat ggttataata tctttagatg caagaatgcc tcctataata
    22201 catatagttg caaatcctat attactaatc acattcataa taggcattaa aagagaagtg
    22261 taaatttgcg atttaaggga aatatttaga attctatcat tgacttcttt aaaatcattt
    22321 acagattttc tttcataatt aaaagatttt actacattta tgtttgatat catttcttca
    22381 atatgtgcat ttagttttcc caactcaact tgttgttgct taaaaaaaac tctagttctc
    22441 tttgcaataa aattactcac aaacattact atcggtatta atattacact gcaaaaggtt
    22501 aaaattgggc ttaatacaaa catcataata aaagtcatca atatgtttaa gattcctgta
    22561 attaactgta ctaatgaatt tgatattcct gtactgatat tatcaacatc atttgtcact
    22621 ctactcataa tatctccagt agaactttta tcaaaaaagg ttattggtag atttttaaat
    22681 tttaaaaata aattacttct catttcttta actatacttt gagataatct agctgtacat
    22741 atccccttag acagtttaac tgaaaactca attatataaa atgaaagtaa aataattatt
    22801 ccttttaaga aaatggattt tgaataagta tcgatatcta ttatgtcaac tacctttcca
    22861 atagtatatg gtattacagt tgatataatt gaatcaatta ttactagcaa aaaaataaat
    22921 ctagtaatct tcttatcttt tttaaagtac ataaaaattc ttttaaacgt ttttttaaaa
    22981 tctttaggct tttctttttt ctttaatcta ctgaccttct gatttccagt acttaaattc
    23041 aatatacttc ctccttaatt tacatcttca cttactaaat tctgcaacgc aaaaattttt
    23101 ttataaattg gagaactaac cagtaaatct tcatgtgtag caaatccaac cacctcaccc
    23161 tcatccaaga ctaaaatctt atcacagtca attatagaac taatcttttg tgaaatagtt
    23221 attacagtac atttcttact ttccttttct cttaaattac ttatatactc ttttatattc
    23281 tttcttataa ttgtctctgt attaatatcc attgcactaa aacaatcatc aagaattaat
    23341 atttgtggtt ttttggctat agctctagct atagtaattc tttgtctctg tcctcctgat
    23401 aaatttttac ccctttggaa taaaacttca tcataagtat tttcgagttt tgttataaat
    23461 tgatttgcat ttgctatttc acatgatttt acaacatcct ctttgcatat atctctattt
    23521 cccataacta cattctccaa tatacttttt ccaaacacac tactattttg aaatactaca
    23581 cctattcttg cccttaaact ttcttcagaa atactgtata tactacgtcc tccaatctta
    23641 atatcacctt tatcaattat atataatttt tgaattaaat ttactaaaga agtctttcct
    23701 gaaccagttt gaccaatcac accaatattt tgaccttctt ctattttaaa ggatacgttt
    23761 tttaatattt tttcatctgt atttttgtta tacgaaaaat ctacattttc aaaaattata
    23821 tcacagttta caaactttaa atctttttct ccaacatcta aactttcttc tctctcaatt
    23881 acttcttcat tctcatcaac caaaacctca cttattctct cataagatgc ttttgcccta
    23941 acaaaaacat taaaaacatg tgaaatcatt ataagagatg ttaatattct agtcatatag
    24001 tttacataag caactataat cccaatttta atttctcctg aataaattaa atgccttcca
    24061 attagtaata ttattattat ggaaacatta atgattattg taattgtcgg tctaaaaaat
    24121 gacatcatag ttgtagcttt tctactattt ttaaaaagtt catcatttaa ctcttcaaag
    24181 ctttggttct cataatcgta tctacaaaat agtttaacta cttttattcc acttaaaaac
    24241 tgtcttaatt ttgaatttac tttatctgta ctttcttgta catttttaaa taatctatac
    24301 cctatcttca tattaagcaa tattataata aatataatta aaactgcaat tagaataatt
    24361 attgacatct ttaaatttaa ttttatagct ataattaaac ttcctataca cattattggt
    24421 gactttataa aaactctcat cattccatta acgaattgtt gcatttgatt gatatcatta
    24481 gtaattcttg ttattaatga agctctacca aatttatcta tttgttttaa tgagtatgta
    24541 tttatctttt caaatacatc ctgtcttaaa tcataagaaa aatgctgaga aactcttgcc
    24601 gagattacac ttcttattat tgcaaatatt gcacctaaaa aagttatacc tatcattata
    24661 gctcctgttg atagtatgta gtctaagttc ttatttgaaa cccctatatc tattattttt
    24721 gccattatag ttggttgagc taaatcacaa aaagcttcca atgttaaaaa aaatactgct
    24781 gttagaaata atactatata tttttttatg tattttttaa aatacctcat tcaatcatcc
    24841 cttcatagaa ttttctcatt accaaaagta tatcataatg gctaatttaa ttgattatat
    24901 caattataaa tatttgattt attattaatt tttataccat atattttata aattatttgt
    24961 ttttatttcc tatatttata tatatttata
//
LOCUS       DAMJYR010000050        24566 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.050, whole genome shotgun sequence.
ACCESSION   DAMJYR010000050 DAMJYR010000000
VERSION     DAMJYR010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 24566)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24566)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24566
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.050"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            341..1162
                     /gene="yidA"
                     /locus_tag="PC471_001968"
     CDS             341..1162
                     /gene="yidA"
                     /locus_tag="PC471_001968"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HDF2789098.1"
                     /translation="MYKLIALDIDGTILNTQKRITPEVFESIQEAKRAGAKVVITTGR
                     PLPGVKELLNQLNLTDEGDYVICFNGAIIQEVKSEKIIHDVEMSLDDFDFIYNNVCKK
                     YKTKIHINTMTNLITPNETPGKYTLHEAKLNNIEVKYIQKDKIDESIKICKIMIVDEP
                     ERLEEIIQQLPKNLFNKYTIVRSAPFYLEFLGKTTNKGTALKTLCTNLNIPIENAIAV
                     GDEENDQHMIKYAGLGVAMGNARNSIKEIADYVTDTNNENGVAKVINKYILNKAI"
     gene            complement(1298..1447)
                     /locus_tag="PC471_001969"
     CDS             complement(1298..1447)
                     /locus_tag="PC471_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2789099.1"
                     /translation="MNFFWTKSDFDAWTNEAGLSDDEDIYCLDINEAIVESYKIFKLK
                     QKVLS"
     gene            complement(1454..1939)
                     /locus_tag="PC471_001970"
     CDS             complement(1454..1939)
                     /locus_tag="PC471_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2789100.1"
                     /translation="MSQIEKYNSLDNTQQKIRISIMDMIIDKGSSVTLQEVTEYVSKK
                     LNIEKEYIERTLQYFIYKNIMVVDGNSINFIYPVSALPTNHKVTLRDNRSFSAMCAID
                     AIGTSCTFNQDIKINSICSATGKEIEVIIKNEKIEYVNNPNLRVLHINLDKHLNWAAS
                     C"
     gene            complement(1952..2704)
                     /locus_tag="PC471_001971"
     CDS             complement(1952..2704)
                     /locus_tag="PC471_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2789101.1"
                     /translation="MKLSVRDINKTFVNNRVHTKVLEDISMDIDDGQFVCLLGPSGCG
                     KTTLLTIIGGFQKSERGDVFINDKRVKKPGIDRAFIFQNYALFPWKTIRGNVLFPMKQ
                     QKIPKEKREEMLEELLVMSDLKGKEKLFPHQLSGGMKQRVAMIRALACNPEVLLMDEP
                     LGAVDFQMRQNLQEELERIWIKKKITALMVTHDVDEAVYMSDRVIVMSRDKGRIIDDI
                     NIDIPRPRIRGSQKYEEYKNKLTDTLSKCYEV"
     gene            complement(2881..3510)
                     /locus_tag="PC471_001972"
     CDS             complement(2881..3510)
                     /locus_tag="PC471_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2789102.1"
                     /translation="MEIKISKDKSDRLTKKKTDGSMDILYKIILLVIIFLVWHFAAKD
                     IGSSLLLPMPVDVIKGFFFCVTDAETVTNLFITLQRVLKGFMYALLFGLPIGFIMGFS
                     KTFERVLSPVVDSVRQVPIMAWVPLTIVWFGIGDGPTIFLIAFSGVFPIILNTIQGVR
                     AISKDYYNAARSMGASPIVIFTNVIVPASLPDILTGSRIAISTGWMSVI"
     gene            complement(3471..4034)
                     /locus_tag="PC471_001973"
     CDS             complement(3471..4034)
                     /locus_tag="PC471_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2789103.1"
                     /translation="MQWALSSEEIDIAIICKDAAKQYVNVNSGFEIVGTVVQNSDIFL
                     LSDNNPEKIGVIQNRDYQSELVKKYYKNVEVAPLLGTALAYGLESNLVDGVVIDCIKS
                     IGLEGKRKSTTKLGDYDTYVLVVNKEFKKSKAYSDFITLYNKSVDALKNKATFKKVLK
                     EYRNIRNIDMEEISNWKLKFLKIKAID"
     gene            complement(4044..4268)
                     /locus_tag="PC471_001974"
     CDS             complement(4044..4268)
                     /locus_tag="PC471_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2789104.1"
                     /translation="MENMKNIIILFIIILGSIFIIFFNFEDKEEIKGKLRIGVSDDTS
                     GFVINYMVNRDYFENIKVSDIMDIFTINDC"
     gene            complement(4273..5364)
                     /locus_tag="PC471_001975"
     CDS             complement(4273..5364)
                     /locus_tag="PC471_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDF2789105.1"
                     /translation="MKLSKKIQALILFGVMGTSILTGCSSKPKEKEEAKTGKDDYTIT
                     VGYYNCDHMTAGPVAESAGIYKDLGLNVKTVGNGKVPEAMAAGKMDAGYIGTKGLVGA
                     IPKGSPITIAANNHTGGSEYLIVSKDIKEPKDLIGKKIATDMSDFLWTSDYGPETGLP
                     TDPSKYEVVNMDSDKDKYLALKTGKIQAFTSCDPWGSVAENDGAGKIIASTQYKEKAN
                     GKEYNCCSFSLNKNFIKEHPDLAKKLVLAHTKAIEYIYTNPAEAAKIFAKYYNVEEEV
                     ALRTIYKKTVGEGRTLTWKVTGEEYKNNLQMYKDLKALDDIPKYEDTIDTSLLDSCGA
                     DDFDKFIKEKVNPEFPEGMSYEDWKAKVL"
     gene            complement(5399..5857)
                     /locus_tag="PC471_001976"
     CDS             complement(5399..5857)
                     /locus_tag="PC471_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2789106.1"
                     /translation="MLKKIVIISCIVVVLIILSKIVDDNIKEDASIPNVNKETLEYFR
                     KNYKEDIITCAEEDLNNDGKKDLVVIYKKSNNSNEMVVVVSDKNSHYITKPIPAPIEN
                     QTITFKNIDDKAPIEVIVSGSKNGNVGYAIYRVEGKKFVDLFGEDMDKCC"
     gene            complement(5850..6935)
                     /locus_tag="PC471_001977"
     CDS             complement(5850..6935)
                     /locus_tag="PC471_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HDF2789107.1"
                     /translation="MNILEIVLNGVKDTIVASIVTLNGASGWLMISFILAGVLHNVLT
                     PRRFQKQLGNKKFSSLIKSTISGMFLPICSCGVIPLGISMYYSGAYLGPVLAFMTSTP
                     IINPIAVVLCLGLLGPKVTIIYLIAGFLVPFLVGFLGNCFGKEELSLHNDSEEEVIEL
                     EEEKRSFMGNMIDGLKWSFGELALTISKYVVMGMVFAGFITTIFPNSIIQKYLGNPGL
                     LSLGSIAILACVMYVCAVGHIPFIAALVASGASPGIAITFLIAGAATNLPELISMFKL
                     IGKRTVIIYSVTLTLSSIIIGYITNLLLVNNNSVNLDATNSSIQLANKLMLNIPDSFE
                     YVCTILIILFAFKSIIPKMKEVIGNHA"
     gene            complement(6962..7147)
                     /locus_tag="PC471_001978"
     CDS             complement(6962..7147)
                     /locus_tag="PC471_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2789108.1"
                     /translation="MSEKYADKLEIKLYQAGKDFSYIKKYGIITKGTLIINQKKKYDR
                     LNKDTIERAIVEAINNN"
     gene            complement(7309..7641)
                     /locus_tag="PC471_001979"
     CDS             complement(7309..7641)
                     /locus_tag="PC471_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HDF2789109.1"
                     /translation="MKVAYIFSSTNTHKILDKMIIPQLEQGTHGVDVLGMFFFMDNTL
                     FLSKGNPVGERLSKLHEKTDMIIMACDQCAIERGIENNLVDGATIGCFPNLYACLGSA
                     GVDQVITL"
     gene            complement(7897..8733)
                     /gene="nadC"
                     /locus_tag="PC471_001980"
     CDS             complement(7897..8733)
                     /gene="nadC"
                     /locus_tag="PC471_001980"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HDF2789110.1"
                     /translation="MNYLIIDRMIQDALIEDVPSEDITTNSIVDENSKSTVDLICKQD
                     GIIAGLGVFKRVFEILGDVDVKLYKNDGDRVKNREKIAVLTGSTRNLLVGERVALNYL
                     QRMSGIATITSRYVEKLEGTNTKLLDSRKTIPNLRILDKYSVKVGGGCNHRFNLSDGI
                     LLKDNHIGAAGSVKKAVELARKNTSFVRKIEVEVETLDMVKEAIEANADIIMLDNMSL
                     EMAKQAVDIINGRAIVEFSGNVNLDTIEDIGKIGVDVVSVGALTHSVKALDISMKNLR
                     NI"
     gene            complement(8714..10015)
                     /locus_tag="PC471_001981"
     CDS             complement(8714..10015)
                     /locus_tag="PC471_001981"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HDF2789111.1"
                     /translation="MNLEQDVLIVGSGVSGLYCALNLDKSLNVLVVSKSTIENNNTYL
                     AQGGISTARNIDDIESFVEDTMKAGQYKNRVEAVQVLADESIENVGQIVEYGMPLDKE
                     NGEIDYTREGAHSVNRIVHSKDNTGEVVFKTLLKEAKTRENITLIEDAYLLDILKDGN
                     KCIGARIFKSKKEIHVFSKIVVLATGGIGGLFKNSTNQRHLTGDGIAIALRNNIKIEN
                     LEYIQIHPTAFYEENNEGRRMLISESLRGEGAKLLNKNKERFVDELLPRDVVSKAIFE
                     QMEKDKLPYVYLDATHLDSEYLINRFSFIYNECLARGTDITKECIKVSPAQHYFMGGI
                     HVDLDSKTSMENLYAVGEISCTGVHGANRLASNSLLEGLVFSKRAAKNINSVIDNVKV
                     KFIDVPDMDIDIEQVKKENKILVIKEIERTSEDFGDELFDY"
     gene            complement(10028..10942)
                     /gene="nadA"
                     /locus_tag="PC471_001982"
     CDS             complement(10028..10942)
                     /gene="nadA"
                     /locus_tag="PC471_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HDF2789112.1"
                     /translation="MDKDLTYQIKELKKEKNAIILAHFYQPPEIQELADAVGDSYYLS
                     EIARDCKEEVVVFCGVRFMGESAKILSPEKTVLMPVSNAGCAMADMVDEEGVIKLKQQ
                     YPNALVVCYINSTAKVKAHCDVSVTSSSAIKILENIDNKEIIFLPDKNLGGYIAEQFP
                     DKNFIFWDGYCKYHNNIRAEEIIELKDKYKNAEVLVHPECKKEIRDLGDYVGSTSGII
                     KYATNSKNKDFIIATEEGILHELKKNNPNKNFYIPGGKILCTDMKKTTLENLYSTLKN
                     MENEVIVEDEIMEKALNSLLNMHKLAEG"
     gene            11360..12817
                     /locus_tag="PC471_001983"
     CDS             11360..12817
                     /locus_tag="PC471_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein A"
                     /protein_id="HDF2789113.1"
                     /translation="MTTFLIGLAILLIGGALYGAYCEKVFGPDDRKTPALAQSDGVDY
                     VPMKKWKNSLIELLNIAGTGPILGPIQGILFGPIAFILIPIGCVFGGALHDYMSGMIS
                     IREKGAQMPSLISRFLGNKVFQVYNIFLCLLMLLVGAVFIYTPGDLVVTQILNMKSTI
                     NNPVVWIVYGLIFLYYLCATLFPIDKIIGKVYPIFGAILLLSAAGVGIGIFTQGYDLA
                     NLSLANWKGIHPDGIPLIPTFFVTVACGIVSGFHSTQATLIARSVSNEKEGKTTFYNM
                     MILEGLIAMIWAAAAMGIYNKGIPKELVGSPDVIGLVARDLLGSIGGIIAIIGVIVLP
                     ITSGDTALRSLRLMLADYFHYDQKEKKHRVILSICIFIPVIAILIFAKLSASGFNILW
                     RYFSWSNQTIAIFAFAMITVYLIIKEKNYIISLIPGMFYSFVIFSYIFNAQIGFNLNI
                     NISYVLAAIFTVLYAILTVRSGRKLKSKADTKLAD"
     gene            13040..13468
                     /locus_tag="PC471_001984"
     CDS             13040..13468
                     /locus_tag="PC471_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2789114.1"
                     /translation="MNNKLHELEKNLPETSCSFCTHLSFKGPSLDYRYDIRCVISDTK
                     PDFRGCCEYFEPEYTELNTGDLDNLYINFLETCLKVNYNDYINSMYWKLFKERTLLEN
                     NFKCSICGSTENVDVYHVNKNLGRETSKDVVVKCDKCNCK"
     gene            13528..13836
                     /locus_tag="PC471_001985"
     CDS             13528..13836
                     /locus_tag="PC471_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1540 domain-containing protein"
                     /protein_id="HDF2789115.1"
                     /translation="MKNNNLNCAATNCAYNTSGYCYAGSIKVDGMQATTTENTYCASF
                     EDKYTSGITSRSNETNQVDTDNIHCEAVKCKYNKNELCKAEKVHINERNASCETFEMK
                     "
     gene            complement(13983..15005)
                     /locus_tag="PC471_001986"
     CDS             complement(13983..15005)
                     /locus_tag="PC471_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HDF2789116.1"
                     /translation="MEDNKGLARDSSKYKIVSISVVVIILITFWYMLNMVLLTFIMTF
                     IFYNLLVATRKRIKKFSSLNIPDSLIIIVLYALFAILLVLISYAVVPIIIVQLTELSR
                     VFSDFDVNQFAQSLGPKLYPIVSKLDFNKYISQAGLLIASTATKVGSFGVNILLAFLL
                     SLLLLLEKNEIKNFGDKLSDSKISFIYNSLVFFGKSFVKNFGEVMKVQVMIAFINSVV
                     SMIFLGFMGFPQIWALGFMIFVLGLIPVAGVIVSLIPLTVIAFNTGGITKVFGVLLMI
                     CIVHAVETYILNPKLMSNRTKLPVCFVFIILLVGEHYLGVWGLLIGVPIFMFLMDILG
                     VKFTSR"
     gene            complement(15180..15686)
                     /locus_tag="PC471_001987"
     CDS             complement(15180..15686)
                     /locus_tag="PC471_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HDF2789117.1"
                     /translation="MELWDLYNADGIKTGNVIKRGNSIEEGYYHLAVEVWILNSNSQI
                     LIQKRSKSKKTLPNMWGMTTGCIVSGEDSLEGAIREAKEEIGIDITKDEMKVFRSMIH
                     EDTLWDVYLVKKEYDISKAILQEEEVSDIKWVSTDEIRQLLKEGLFFEYPEIYELLYE
                     IDNNKLNI"
     gene            complement(15746..16447)
                     /locus_tag="PC471_001988"
     CDS             complement(15746..16447)
                     /locus_tag="PC471_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2789118.1"
                     /translation="MGKISLKSMIRLFSGFFIFAISSVLMINAHVGLMPWDVLHQGLS
                     IKLGITIGQASIMVGVVIVILDAVFGENIGWGTLLNMTFIGIFIDLVIFSGVIPHASN
                     TYIGVFMVVIGIILAAIASFLYLGVCLGSGPRDGLMIALQKKTNKSVRLVRTILEILA
                     LVVGWLLGGSVGIGTLVSALGLGYVLQIVFRIFKFDTKLLKHRFIIDDIREWKEKKSN
                     EHKCKSSIVIKNEQN"
     gene            complement(16665..17735)
                     /gene="buk"
                     /locus_tag="PC471_001989"
     CDS             complement(16665..17735)
                     /gene="buk"
                     /locus_tag="PC471_001989"
                     /EC_number="2.7.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="butyrate kinase"
                     /protein_id="HDF2789119.1"
                     /translation="MTYRILAINPGSTSTKIAVYDGEEQILVKTIDHPAEEIAKYNTI
                     QDQFEMRKEAVLNILKENSIDLKSLSAIVGRGGVLPPVKSGAYLVNEEMIDVLRHRPV
                     LEHASNLGAVVAHAISEPLGINSYIYDSVAVDELIDVARISGLCGMDRSSAGHALNTR
                     AMALKYAKDKGKDYKSLNLIVAHIGGGVSIYLHEKGRMVDMLSDDEGPFSPERSGRVP
                     ATKLVAACYSGQYSEREMTKKIRGKGGIVSYLNTVDAREVEKMIAEGNEEAKIIYEAM
                     AYQLAKGIGELATVVDGKVDAIIITGGIAYSEMFTSMVKKKVEFIAPVEIMAGENELE
                     SLAFGTLRVLNGEEEARIYSEN"
     gene            complement(17788..18363)
                     /locus_tag="PC471_001990"
     CDS             complement(17788..18363)
                     /locus_tag="PC471_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate oxidoreductase subunit beta"
                     /protein_id="HDF2789120.1"
                     /translation="MTKSILLVGVGGQGTILASKLLTMGLMEAGYDVKMSEIHGMSQR
                     GGSVSSQVRYGDCVHSPVIEIGGADILVSFEKMEALRWFNYLKPEGKALVNNHRIDSM
                     TVLIGGAEYQESDIDAELSRLNAKVINAADKAEELGNSKIMNVILLGCLVKSMQLESI
                     DWEKIISENVKPKFVELNIKAFHEGMEMVSK"
     gene            complement(18365..20152)
                     /gene="iorA"
                     /locus_tag="PC471_001991"
     CDS             complement(18365..20152)
                     /gene="iorA"
                     /locus_tag="PC471_001991"
                     /EC_number="1.2.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase subunit
                     alpha"
                     /protein_id="HDF2789121.1"
                     /translation="MKQLMTGNEAIARGAYEAGVKYASAYPGTPSTEILENIATYKDA
                     IVAEWAPNEKVALEAAIGGSIAGARTMASMKHVGLNVAADPIFTYAYTGVNGGMVLIT
                     ADEPGMHSSQNEQDNRMYAKFAKIPLFEPSTSQEAKDMIKEAFEVSEKYDTPVLYRVT
                     TRLCHSKGLVECYDREEVEIKEYVKNAKKMVTVPANAQIRRGVVEERMEILKKFSNET
                     DLNYYEINDTKIGVIASGMCCNFAKEVFGKNASYMKLGFTNPLPYEKIKEFAEKVDKI
                     YVIEENDPFIEEQIKAYGIDCIGKDVIPPYGEMTPDVLRKAIFGKTNDTIEYKSELVT
                     PRPPSFCAGCPHRGFFYELGKRKNLIVGGDIGCYTLGFAPPYNGIDYVVCMGSAFGTA
                     HGAQKVLNMKDDNEKRLVGVLGDSTFFHTGINGLLDVVYNRGNSISVILDNRITGMTG
                     HQENPGSGYTLQGAKTKEVDIEGLVKACGIEHVRVINPNNLKEVNEALDWALAIEDEP
                     SVIITRWPCVLKKFSKEDIEEFNNPFKTKCKVDHDKCIGCKLCLKTGCPALSFDKENK
                     LSNIDRNQCVGCGVCAQVCPKQAIVKEEK"
     gene            complement(20213..21472)
                     /locus_tag="PC471_001992"
     CDS             complement(20213..21472)
                     /locus_tag="PC471_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HDF2789122.1"
                     /translation="MSQSMVGKHAMWPKENDVIFSISGRAQAAEKAFGMDNVINATIG
                     ALMDDSGKLITMKTVYEEYKALDNCEIGAYAALEGQPDYLEAVKKVFFRDYLPEGHIR
                     VLASPGGSGAIKLAVWNYTNEGDEVLTSDWFWSPYVSIAEEANRKVVNYQLFDENRRF
                     NFESFKEKFVNIAEKQGRVFTIINTPAHNPTGYSVADDEWDKILDLSKEVAKDKDKKI
                     IFFVDSAYIDFAGDDDVCRKFFKKFSNLPENVLVLVGFSMSKGFTAYGMRMGAIICIS
                     SSEDVAEEFHYSCVHSCRANWSNCNRSAMAVLSNIVNDPKKFKEYEDEKEIYKNMLTR
                     RADVFVKEAERVGLEILPYIAGFFVSIPCDNPKEVCEELTKHNLFAVPLKMGLRFAVC
                     AVSEDKCKKAPSIIKEALESLEVKINN"
     gene            complement(21734..23734)
                     /locus_tag="PC471_001993"
     CDS             complement(21734..23734)
                     /locus_tag="PC471_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDF2789123.1"
                     /translation="MILLRKKIGIIASDIELKERIEELYREDVENGTIIIDILNLDLM
                     ENQGRILVEKGAQAIIGRGGGYSLVIDTVNVPVIPMNMKSTDLLRAIEIAKKYSKKVV
                     LILGDNEVSFDYVGWRNVISTEITEEWFESKYEIRSKVVKYIDQKDEVVIVGGGLACS
                     FARQYGIDSVFATASDESIREAVEYCKKLLDTLGEEKFNNEVLRNILDGIKDGVIAID
                     SNGSIILYNESAKNMLKVERKCALNKYILDVFPKMEWMLDCLHEKEDVEDRKIRNINN
                     LIVNTRTTLIKVDNSTYGVLGIIQDITKLQNLERKIRFDLNQKGLYARYTFDDFLFKD
                     KLTKEFIEEAKKIGKSDYTTLLYGESGSGKEIIAHSIHNISKRKDRPFVAINCATIAE
                     NLLESELFGYEEGAFTGARKGGKRGLFELAHGGTLFLDEINSLSFNIQTKLLRVIEER
                     QIMRIGSDYIIPLDIRIIAATNESLTEKIVMGTFRADLFYRLSSLEINIPPLRDRRED
                     IIPLFNNFVNEVLKDDGLNGINSIDENFVLTKDEMDKLYNYSWPGNVRELKTIAQKYV
                     VTGKIKLRQDRDFKTKKLLPNSEVDKFNSETTASVEVQDESINISKINDGKISIDIKE
                     VNKYVEEKIISMLFAQGLSKNEVAQVLGISRTSLWKKYNKNI"
     gene            complement(23742..24566)
                     /locus_tag="PC471_001994"
                     /pseudo
     CDS             complement(23742..24566)
                     /locus_tag="PC471_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897561.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
BASE COUNT         7951 a         4432 c         2650 g         9533 t
ORIGIN      
        1 tattttataa cacaaaacta actaatataa caaattacca tcagtctatt tattcaaact
       61 tctaactata taatatacat ttatttatat taacaatgca aacgatacca ttatttgtaa
      121 ttaagaattt tggaattatt gattacaaag tttttacatt ctcatctcac ataagtaata
      181 tttttatcaa aaaattaata ttattaaact ttctaaatta cttatctctt gatataatag
      241 tatacaaaac aaaccatatt tcccactgaa actttgttca acactagctg tgtcaacgtt
      301 ttcacaggaa atttgagtat ataatttatt ggaggttgat atgtacaaat taatcgcact
      361 agacattgat ggaacaatat taaatacaca aaaaaggatt actcctgaag tctttgagtc
      421 cattcaggag gcaaaaaggg ctggggcaaa agtagttata acaacaggca gacctcttcc
      481 tggtgtaaaa gaacttctaa atcaactaaa tttaacagat gaaggtgatt atgttatatg
      541 ctttaatgga gcaattattc aagaagttaa aagtgagaaa atcatacatg atgtagaaat
      601 gagtttagat gattttgatt ttatatataa taacgtttgt aaaaaatata agactaaaat
      661 acacataaat acaatgacaa atttaattac accaaatgaa acgcctggca aatatacact
      721 tcatgaagcg aaattaaata atatagaagt caaatatata caaaaagata aaattgatga
      781 atctattaaa atctgtaaaa ttatgatagt tgatgaacca gaaagattgg aagaaatcat
      841 acagcaacta cctaaaaatt tattcaataa atatactata gtaaggtctg ctccttttta
      901 tctagaattt ttaggtaaaa ctactaataa aggaactgct cttaaaacat tatgtacgaa
      961 tctaaatata cctatagaaa atgctattgc tgttggcgat gaagaaaatg accaacatat
     1021 gataaagtat gcaggtcttg gtgtggctat ggggaatgca cgtaatagta taaaggaaat
     1081 tgcagattat gttactgata ctaataatga aaatggagtt gcaaaggtta taaataaata
     1141 tatacttaat aaggctatct aatcatctaa aaaaactaaa aatatacctt tagatttaaa
     1201 tacttttcac atccctactc tatactaatt tatatagtat ggatattacc tatcattttt
     1261 atctaaaggt catttttagt ttttcatata tgtatatcta tgatagtacc ttttgtttga
     1321 gtttaaatat tttatatgat tctactattg cctcattaat atctaaacaa tatatatctt
     1381 catcatcaga taatcccgct tcgtttgtcc aagcatcaaa atcactcttc gtccagaaga
     1441 aattcattat atttcagcaa ctagcagccc aatttagatg cttatcaaga tttatatgaa
     1501 gcactcttaa gtttggatta ttcacatact caatcttttc attttttata attacttcta
     1561 tttccttacc tgtagcacta caaatagagt ttatttttat atcttggttg aatgtgcaag
     1621 aggttcctat agcatctata gcacacattg cactgaaact tctattatct ctcaaagtta
     1681 ctttatgatt tgttggcaat gctgatacag gatatataaa atttatacta ttcccatcaa
     1741 caaccattat atttttataa ataaaatatt gtagtgtcct ttctatatat tctttttcta
     1801 tattcaactt tttacttaca tattcagtca cttcttgaag tgtaacacta gaacctttat
     1861 ctattatcat atccataata gagattctta ttttctgttg tgtattatcc aaactattgt
     1921 atttttctat ttgactcatg tctactcctc tttaaacttc ataacacttt gataaagtat
     1981 cagttaattt gtttttgtac tcttcatatt tttggcttcc tctaattcta ggtcttggaa
     2041 tatctatatt tatatcatca ataattcttc ctttatctct cgacattact ataactctat
     2101 cactcatata tacagcttca tcaacatcat gagttaccat taatgcagtt attttcttct
     2161 ttatccatat tcgttctagc tcttcctgta aattttgtct catttgaaaa tctacagctc
     2221 ctaatggttc atccattagt aatacctctg gattacatgc taaagctctt atcatggcca
     2281 ctctttgttt cattcctcca gataattgat gcggaaacag tttttctttt cctttcaagt
     2341 ctgacataac caaaagttct tctagcattt cttctctttt ttcttttggt attttctgct
     2401 gtttcatagg aaatagaaca ttacctctta tagttttcca aggaaataaa gcataatttt
     2461 gaaatataaa agctctatct atgcccggtt ttttaactct tttatcattt ataaaaacat
     2521 ctccacgttc tgatttttga aacccaccta ttatagtaag taaagttgtt tttccacaac
     2581 ctgatggtcc taaaagacag acaaattgtc catcatctat atccatactt atatcttcta
     2641 aaacctttgt atgtactcta ttatttacaa atgtcttatt tatatctctt acacttaact
     2701 tcattaatct gcaaacctcc atctacaaag gattttattt ataaacttta ataccctatc
     2761 tattataaat cctattaaag ctgcaaatat cataagccca attaatacat ctgtatgcat
     2821 taatgtttga gcttgtacca ttgagtaacc gagaccggca ctcgtagcta taaactcggc
     2881 tcagataaca gacatccatc ctgtacttat agcaatcctt gaaccagtta gtatatctgg
     2941 aagagatgcg ggaacaatta catttgtaaa tatcacaatt ggcgatgctc ccatacttct
     3001 agctgcatta taataatcct ttgatattgc tcttactcct tgaatagtat ttaaaattat
     3061 agggaaaacc cctgaaaaag ctattaaaaa tattgttgga ccatctccta taccaaacca
     3121 tactatagta agaggaaccc atgccattat tggaacttgc cttacagaat ctactactgg
     3181 agataaaacc ctttcaaaag ttttagaaaa tcccataata aatcctattg gaagcccaaa
     3241 caataatgca tacataaaac ctttaagtac tctctgcaat gttataaaaa gattagtgac
     3301 tgtctctgca tctgtcacac agaaaaagaa accttttatt acatctactg gcattggaag
     3361 taacaaagaa ctccctatat cttttgctgc aaaatgccag actaaaaata taataactaa
     3421 tagtattatt ttatataaaa tatccatact accatcagtt tttttcttag ttaatctatc
     3481 gcttttatct ttagaaattt taatttccaa ttgcttattt cctccatatc tatatttctt
     3541 atattcctgt actcttttaa tactttctta aatgtagctt tattctttaa agcatcaaca
     3601 cttttattgt aaagtgttat aaaatcacta tatgctttac tttttttaaa ctctttattt
     3661 acaactaata cataggtatc ataatctcct aatttagttg tagattttct tttaccctct
     3721 aacccaattg actttatgca atctattaca acaccatcta ctaaattact ttctaatcca
     3781 tatgctagtg cagtccctaa taagggagct acttccacat tcttgtagta ttttttaacc
     3841 aattctgact ggtaatctct attttgaatg actcctatct tttcaggatt attatcactt
     3901 aatagaaata tatcagaatt ttgaactaca gtaccaacaa tttcaaatcc actatttaca
     3961 tttacatatt gttttgcagc atctttacat atgattgcta tatctatttc ctctgaactc
     4021 agagcccact gcattgtact tgctcagcaa tcattaattg taaaaatatc cattatatca
     4081 cttactttta tattttcaaa gtagtctcta tttaccatat aatttattac aaaaccagag
     4141 gtatcatcag atacccctat tcttaatttt ccttttattt cttctttatc ttcaaaatta
     4201 aaaaatatta taaatatact tcccaaaata atgataaaaa gtattattat atttttcata
     4261 ttctccattt atttataata cctttgcttt ccagtcttca taactcatac cttcaggaaa
     4321 ctctgggtta actttttctt ttataaattt gtcaaaatca tcagctccac aagagtccaa
     4381 taagcttgta tcaatcgtat cttcatattt aggtatatca tctaaagcct ttaaatcttt
     4441 atacatttgt aaattatttt tatattcttc acctgtaact ttccaagtaa gagttctacc
     4501 ttctccaaca gtcttcttat agatagttct aagtgcaact tcttcttcta cattataata
     4561 ttttgcaaat attttagctg cttcagctgg atttgtatat atatattcta ttgcttttgt
     4621 atgtgctaga accaattttt ttgctaaatc tggatgttct tttataaagt ttttgtttaa
     4681 tgagaatgaa caacagttat attctttgcc attagctttt tctttatatt gagtagatgc
     4741 tattattttt cctgcaccgt cattttcagc tacagaaccc caagggtcac aagatgtaaa
     4801 agcttgaatt ttcccagttt taagagctaa atatttatct ttgtctgaat ccatatttac
     4861 aacttcatat tttgatgggt cagttggtag acctgtttct ggaccataat cacttgtcca
     4921 taagaaatca ctcatatctg ttgcaatttt tttacctatt aaatctttag gttcttttat
     4981 atcttttgaa actattaaat attcagaacc accagtatgg ttgttagctg ctattgttat
     5041 tggcgaaccc ttaggtattg ctccaactaa ccccttagtt cctatgtaac ctgcatccat
     5101 ttttcctgct gccatagctt caggaacttt accatttcca acagttttta cattaagacc
     5161 taaatcttta tatattcctg ccgattctgc tactggtccc gcagtcatat ggtcacaatt
     5221 ataataacct actgttattg tataatcatc ttttccagtt tttgcttctt ctttttcttt
     5281 tggtttacta ctacatcctg ttaatataga tgtacccatt acaccaaata agattaatgc
     5341 ttgaatcttc ttagataatt tcatataaat ttcccacctt tatgtattat ttttattttt
     5401 agcaacattt gtccatatct tcgccaaata aatcaacaaa ttttttaccc tctactctgt
     5461 aaatagcata tcctacatta ccatttttag accctgatac aattacttct attggtgctt
     5521 tatcatcaat atttttaaag gttatcgttt gattctctat aggtgctggt attggctttg
     5581 ttatatagtg gctattttta tcagaaacta cgactaccat ttcatttgaa ttattgcttt
     5641 ttttatatat aactactaaa tctttttttc catcattatt taagtcttct tcagcacaag
     5701 ttattatgtc ttctttataa ttttttctaa aatattctaa tgtttcttta ttcacatttg
     5761 gaatagaagc atcttctttg atgttatcat caactatttt tgataaaatg attaacacaa
     5821 ctactataca ggatattata actatctttt taagcatgat ttcctatgac ctctttcatc
     5881 tttggtatta ttgatttaaa tgcaaataaa ataattaaaa tagtacatac atactcaaaa
     5941 ctatcaggta tatttaacat tagtttattt gccaactgta ttgaagaatt tgttgcatct
     6001 aaatttactg agttattatt cactaaaagt aaatttgtta tatatccaat tattattgaa
     6061 gacagtgtca aagttacaga atatataata acagttcttt ttccaataag tttaaacata
     6121 cttataagtt ctggtagatt tgtagctgct ccagctatta gaaatgttat cgctatacca
     6181 ggagatgctc cacttgcaac aagggctgct ataaacggta tatgtcctac agcacataca
     6241 tacataacac atgcaagtat tgctatactt ccaagagata gcaaacctgg atttcctaaa
     6301 tatttttgaa ttatactatt tggaaaaata gttgttataa atcctgcaaa aaccataccc
     6361 attactacat atttactaat agtaagggct aattcaccaa aagaccattt aagaccatca
     6421 atcatatttc ccataaaact tcttttttct tcttctaatt ctattacttc ttcctctgaa
     6481 tcattatgaa gtgataattc ttcttttcca aaacaatttc ctaaaaatcc tactaaaaat
     6541 ggtactaaaa aaccagctat tagataaatg attgtaactt taggacctaa aagacctaga
     6601 caaagaacta cagctattgg atttattatt ggagttgatg tcataaaagc aagtactggt
     6661 cctaaatatg ctcctgaata atacatactt attccaagtg gtattactcc acaactacat
     6721 attggtaaaa acattcctga gatagtactt ttaattaaag aagaaaattt cttatttcca
     6781 agttgttttt gaaaccttct tggagttaaa acattgtgaa gtacacctgc caatataaaa
     6841 cttatcataa gccatcctga tgctccattc agtgtaacta tagaagctac tatagtatct
     6901 ttaactccat ttaaaacaat ttctaatata ttcatttttg atactcctaa tttttaaact
     6961 tttaattatt gtttatagct tctactattg ccctttcaat tgtatcttta tttaatctat
     7021 catatttctt tttctgattt attattaaag ttccttttgt tataatccca tattttttta
     7081 tatacgaaaa atctttccct gcttgatata attttatttc caatttatca gcatattttt
     7141 ctgacatttt ttttataaac actccatgtt catcacaaga tggtcaagtg tttataaatt
     7201 caactaaaat tttagacatt tgttaatccc ctactttaaa caatttatct tttacttaaa
     7261 ataatccaca gaaaataaat ttttctatgg attgtatcct atactaattt ataacgttat
     7321 aacttggtcc acacctgcac tgcctaaaca agcatatagg tttgggaaac atccaattgt
     7381 agctccatca accaaattat tctctatacc tctttctata gcacattggt cacaagccat
     7441 tattatcata tcagtttttt catgtaattt acttagtctc tcgcctacag gattaccttt
     7501 agataaaaat aaggtattat ccataaagaa aaacatccct agaacgtcta ctccatgagt
     7561 tccttgttca agttgtggta ttatcatttt gtctagaatt ttatgtgtat tagtactact
     7621 aaaaatataa gctactttca tttttattcc ccctatactt tttatatttt acaaaatttt
     7681 acagatacaa ttatattata agtttattct tatttatctg taaaatagat attaactata
     7741 ttattaaaag tgtatatagt atttatctat ttttattaaa ttattgattg atttttttct
     7801 atttttatta attttaatga ataaaaaata gactccttaa atttataaaa caaatttaag
     7861 gagtctattt taattaagtt tatttcaata taaaatttat atatttctca aattcttcat
     7921 acttatatcg agagctttaa cagaatgtgt aagcgctcct acagaaacaa catccacacc
     7981 aatttttcct atatcttcta tagtatctaa atttacattt ccggaaaatt ctacaatggc
     8041 tcttccattt ataatatcta ctgcttgttt tgccatttcc aaactcatat tatccaacat
     8101 tattatatct gcattagctt ctattgcttc ttttaccatg tctaaagttt ccacttccac
     8161 ttctatcttt ctaacaaaag aagtattttt tctagctaat tcaactgcct tcttaacact
     8221 accagcagcc ccaatatggt tgtctttaag caatattcca tcagataaat taaatctatg
     8281 attgcatcct ccacctactt taacagaata cttatctaat attctaagat taggtatcgt
     8341 ttttctacta tctaataatt ttgtatttgt accttcaagc ttttcaacat acctacttgt
     8401 aatagtagct ataccactca ttctctgtaa ataattcaat gctacacgct ctcctacaag
     8461 taagtttcta gtactacctg ttaatacagc tatcttttca cgattcttta ctctatcacc
     8521 atcattttta tataatttta catctacatc accaagtatc tcaaaaactc tcttaaatac
     8581 acctaagcca gctattatac catcttgttt acaaattaaa tctacagtag atttagaatt
     8641 ttcatccaca atagaattag ttgttatatc ttcacttgga acatcttcta tcaaagcatc
     8701 ctgtatcatt ctatcaataa tcaaataatt catcgccaaa atcctcactt gttctttcaa
     8761 tttcttttat gactaatatt ttattttctt tttttacctg ttctatatct atatccatat
     8821 ctggtacatc tataaactta acttttacat tatctataac tgagtttata tttttagctg
     8881 ctctttttga aaatacaagc ccttcaagta atgaattact tgccaatcta ttggctccat
     8941 gaacacctgt acaactaatt tcaccaactg catataaatt ttccatagat gtttttgaat
     9001 ccaaatctac atgtatcccg cccataaaat aatgttgtgc aggagatacc tttatacatt
     9061 ctttcgttat gtctgttcct ctagctaaac attcattata aataaaagaa aatctattaa
     9121 ttaaatattc actatctaaa tgtgttgcat ctaaatacac ataaggtaat ttatcttttt
     9181 ccatttgctc aaaaatagct tttgaaacta catctctagg aagaagttca tccacaaatc
     9241 tttctttatt tttatttaaa agtttagctc cctctcctct taatgattca gatataagca
     9301 ttctcctacc ttcattattt tcttcataaa aggctgtagg atgtatttga atatattcta
     9361 aattttctat ttttatatta tttcttaaag ctattgcaat tccatcacct gtaagatgtc
     9421 tttgattagt agaattttta aaaagcccac caattccacc tgtagctaaa actactattt
     9481 ttgaaaaaac atgaatctct tttttagatt taaaaattct agctcctata catttattgc
     9541 catcttttaa tatatctaaa agataggcat cttctataag agttatattt tctctagttt
     9601 tagcttcttt taataaagtt ttaaaaacaa cttctccagt attatcctta ctatgaacta
     9661 ttcgatttac actatgtgct ccttctcttg tgtaatctat ctctccattt tctttgtcta
     9721 aaggcatacc atattctact atctgcccta cattttctat agattcatct gcaagtactt
     9781 gaactgcttc aactctattt ttatactgcc cagccttcat tgtatcttct acaaaggatt
     9841 ctatatcatc aatatttcta gcagttgaaa ttccaccttg tgcaagatat gtattgttgt
     9901 tttcaattgt tgatttcgat actactagca catttaaact tttatctaaa tttaatgcac
     9961 agtataatcc tgaaactcca cttccaacta ttaaaacatc ttgttctaaa ttcataagtc
    10021 tatccctcta tccttctgct aatttgtgca tgttaagcag tgaatttaaa gccttttcca
    10081 tgatttcatc ttctacaata acttcatttt ccatattttt aagtgtacta tataaatttt
    10141 ctaatgttgt ctttttcata tctgtacata gtatttttcc accaggaata taaaagtttt
    10201 tattaggatt atttttcttt aattcatgta atataccttc ttcagttgct attataaagt
    10261 ctttgttttt actattagta gcatatttta ttattcctga tgtactacct acataatctc
    10321 ctaaatctct tatttctttt ttacattctg gatgtactaa tacttctgca tttttatact
    10381 tatcttttaa ctcaattatt tcttcagctc ttatattgtt atggtattta caatatccgt
    10441 cccaaaatat aaagttttta tctggaaatt gttctgcaat atatcctcct aaatttttat
    10501 ctggaaggaa tataatttct ttattatcta tgttttctag tattttaatt gcactagaag
    10561 atgtaacaga aacatcacaa tgtgccttaa ctttagctgt agagtttata taacatacaa
    10621 ccaaagcatt tggatactgt tgttttaatt ttatcacgcc ttcttcatct accatatctg
    10681 ccatagcaca cccagcgtta cttactggca tcaatacagt tttttcaggt gataatatct
    10741 tagcactttc tcccataaac cttactccac aaaatacaac cacttcttct ttacaatctc
    10801 tagcaatctc acttaaataa tatgagtcac caactgcatc tgctaattct tgtatttctg
    10861 gtggttgata aaaatgggca agtattattg catttttttc tttctttagc tctttgattt
    10921 gatatgttaa atctttatcc ataattgcac cgctttctat atgtactagt gtatatacac
    10981 ctgtaaactc attatatcac ttgactattc tttcttcaag aattttaaac ctcttaatat
    11041 tgttaagata tagacattta tattctcaat tgattttcct tttttctcca tctatttaaa
    11101 ctaattttta tattttttaa tttttttgta aaatattatc agaattttgt ctatattctt
    11161 tattgctata acattgaaaa tattaccaaa tacctgtata ctaagtacat ggttatgtat
    11221 atgtatttat ttctgaaatt atattagttg taatacttta tagaatttgt aggttgtcca
    11281 gaattaaatt caatataaat ctacacattt agataatatg tatacaaaga ccaaaaaatt
    11341 ttataaatgg ggtgatatta tgacaacatt cttaatcggc ttggctatat tactaatagg
    11401 aggtgcttta tacggtgctt attgtgaaaa agtatttggc ccagatgaca gaaaaacacc
    11461 tgcacttgct caaagtgatg gagtagacta tgtcccaatg aaaaaatgga aaaatagtct
    11521 aattgagttg cttaatatag ctggtacagg accaattcta ggacctatcc aaggtatatt
    11581 gtttggtcca atagcattca tactaatacc tattggctgt gtatttggag gagctttgca
    11641 tgactatatg agtggaatga ttagtataag agaaaaagga gcacaaatgc cttctcttat
    11701 cagtagattt ttaggtaata aagtatttca agtctataat atattccttt gtcttttaat
    11761 gcttttagtt ggtgcagttt ttatttatac acctggggat ttagttgtta ctcaaatttt
    11821 aaatatgaaa tcgactataa ataatccagt tgtctggata gtttatggac ttatctttct
    11881 atattacttg tgtgcaacac tatttccaat tgataaaatc ataggaaagg tatatccaat
    11941 atttggagct attctacttt tatctgctgc tggtgttgga attggaatct ttacacaagg
    12001 ttatgattta gctaatttaa gtcttgcaaa ttggaaaggt attcatcctg atggtattcc
    12061 tcttattcct acattctttg ttacagttgc ttgtggtatt gtttctggat tccactcaac
    12121 tcaagctact cttatagcaa gatcagtttc aaatgaaaaa gaaggaaaaa ctacttttta
    12181 taatatgatg attttagaag gtttaattgc aatgatttgg gctgcagctg ctatgggtat
    12241 ttacaataaa ggaataccta aggaattagt aggttctcct gatgtaatag gtttggttgc
    12301 aagagattta cttggttcta ttggtggtat catagctata ataggtgtaa ttgttttacc
    12361 aataacttct ggagatacag ctttaagatc tttaagatta atgcttgcag attactttca
    12421 ttatgaccaa aaggaaaaaa aacatcgtgt tatattgtca atctgtatat ttattcctgt
    12481 catagccatc cttatatttg caaagcttag tgcttctgga tttaatatat tgtggagata
    12541 cttctcttgg agtaatcaaa ctattgccat atttgcattt gcaatgatta cagtttacct
    12601 tataataaaa gaaaagaatt atattataag cttaattcct ggtatgtttt attcctttgt
    12661 tatattcagt tatatattta atgctcaaat tggatttaac ttaaacataa atatatcata
    12721 tgttttagct gccatattta ctgtattata tgctatttta actgtacgct ctggtagaaa
    12781 gttaaaaagc aaagcagata ctaagttggc tgactaaatc taaataaaat aatacttaga
    12841 ataaatttta ttaaaaaatg ataaaaaagt aggcaatctc aagttagatt tgcctctttt
    12901 ttatttaata cagtaataaa tacttaagta tttactcctt attggaatat ttatcctaaa
    12961 tctctaatat aaataaataa aagttttaaa ataatctatt cgtttatata cttaaaataa
    13021 attttttgga ggaatactta tgaataataa attacatgaa cttgaaaaaa atctaccaga
    13081 aacatcttgt tctttttgca cacatctttc atttaagggg cctagcctag actatagata
    13141 tgatattaga tgtgttattt cagatacaaa gcccgatttt agaggatgtt gtgaatattt
    13201 tgaaccagaa tatacagaat taaatacagg agatttagac aatctataca taaactttct
    13261 tgagacatgc ctcaaggtaa attataatga ttacatcaat tctatgtatt ggaagttatt
    13321 taaagaaaga accctactag aaaacaattt taaatgttct atatgtggat caactgaaaa
    13381 tgttgatgtg tatcatgtta ataaaaattt aggaagggaa acttccaaag atgtagttgt
    13441 taaatgtgat aaatgtaatt gtaaataagt gtataaaatt tagataactt cacataataa
    13501 gtttaaataa aacttaggag gttatttatg aaaaataata atttaaattg tgctgctact
    13561 aattgtgctt ataatactag tggatattgt tatgctggta gtataaaagt agatggaatg
    13621 caagcaacta ctacagaaaa tacttattgt gcttcttttg aagacaaata tacatctggt
    13681 ataactagtc gttctaatga aactaaccaa gttgatacag ataacattca ttgtgaagct
    13741 gtaaaatgta aatacaataa aaatgaactt tgcaaagctg aaaaagttca tataaatgaa
    13801 agaaatgcaa gttgtgaaac ttttgaaatg aaataaattt ctttttattt agttttatag
    13861 tatttataca aagcaaaatt gaacctatca aatagatgtc tttattaatg atgtctattt
    13921 ataggttctt ttttaaaaca atttataatt tatatataaa gataaattat tattttaata
    13981 tattacctag aagtaaattt aacacccaga atatccataa ggaacataaa tattggaact
    14041 ccaattagta atccccatac acctaaataa tgttctccta caagtaaaat aataaaaaca
    14101 aaacaaactg gaagtttagt tctattcgac atcaacttag ggtttaatat gtatgtttct
    14161 actgcatgca ctatacaaat cataagtaaa actccaaaaa cctttgttat tcctcctgta
    14221 ttaaatgcta ttacagttaa tggaatcaaa gacactataa ctcctgctac tggaataaga
    14281 cccaacacaa aaatcataaa tcctaaagcc catatttgag ggaatcccat gaaacctaag
    14341 aaaatcatag aaacaacaga atttataaag gcaatcataa cttgtacttt cattacttca
    14401 ccaaaatttt taacaaagct ctttccaaaa aatacaagcg aattataaat aaaagaaatc
    14461 ttactatcag aaagtttatc tccaaaattt ttaatttcat ttttttcaag taaaaggagt
    14521 aaactcagta aaaatgccaa taatatattt actccaaaac ttccaacttt tgttgctgtt
    14581 gatgctataa gtagccctgc ttgtgaaata tatttattaa aatccagctt tgagacaata
    14641 ggatacaatt ttggtcctaa agattgagca aattgattta catcaaaatc actaaaaacc
    14701 ctactcaact ctgtaagttg aactataatt attggtacaa ctgcatagct aatcaatact
    14761 aacaatatag caaatagtgc atataacact ataattatta atgaatctgg aatatttaaa
    14821 gaagaaaact tcttaattcg ttttcttgtg gctacaagca agttatagaa aataaaagtc
    14881 ataataaatg ttaaaagcac catatttaac atataccaaa atgtaattag aataattact
    14941 acgacagata tagaaacaat tttatatttt gaactgtcac gtgccaatcc tttgttatct
    15001 tccatagttt aaatcctccc caaattttta tgactttata atgactgccc tttttatttt
    15061 aatctcaaaa aacaagtaga aaaatactaa aaataactta ttaattttca taaaacaata
    15121 ttaaaattta gagaagatac attttaaata atcttactta tactgaaatc tgctctacat
    15181 catatattta atttattatt atctatctca tatagcaatt cgtatatctc tggatattca
    15241 aaaaacaaac cctcttttag taattgtctt atttcatctg tgcttaccca ctttatatca
    15301 cttacttctt cctcctgtaa aatagctttg gatatatcat attctttttt aactaagtat
    15361 acatcccaaa gagtatcttc atgaatcata cttctaaaaa ctttcatctc atctttagtt
    15421 atatctatcc ctatttcttc ctttgcttcg cgaattgctc cttctaaact gtcttcccca
    15481 gaaacaatac atccagttgt cattccccac atatttggca atgtcttttt acttttagac
    15541 cttttctgaa ttaaaatttg agagttacta tttaaaatcc acacttcaac tgccaaatgg
    15601 taataacctt cttcaataga attacctctt tttataacat ttcctgtttt aattccatca
    15661 gcattatata aatcccataa ttccatttca aaatctcctt gactttacta taaattagat
    15721 aaactacaaa tatattttag cataattaat tctgctcatt ttttattaca atacttgatt
    15781 tacatttatg ttcattgctt ttcttttcct tccattctct tatatcatct ataataaatc
    15841 tatgtttcaa caattttgtg tcaaatttaa atattctaaa cactatttgt aatacatatc
    15901 ctaaacctaa agcacttaca agcgtaccaa tacctacaga acctcctaat aaccacccaa
    15961 caactaatgc tagtatttca agaattgttc taacaagacg tacagattta ttagtttttt
    16021 tctgaagagc aatcataagc ccatctctcg gtccacttcc taaacaaaca cctaagtata
    16081 gaaaacttgc tatagctgcc aaaataattc ctataacaac cataaacaca cctatataag
    16141 tgttgcttgc atgaggtatc actccagaaa atattactaa atcaataaaa attcctataa
    16201 atgtcatgtt gagtaaagta ccccatccta tattttcacc aaaaactgca tccaatataa
    16261 caattacaac tcctaccatt atagaagcct gacctatagt tatgcctagc tttatagata
    16321 aaccttggtg aagtacatcc caaggcataa gacctacatg tgcattaatc ataagcacag
    16381 aagaaattgc aaatataaaa aaacctgaaa ataatcttat catacttttt aaacttattt
    16441 ttcccatcaa aaatcactcc tcataataat aataacaata aataccaata aaatatatat
    16501 ataaatacga aattttacag aattatttat taggttaaga taaaatttta attataaaaa
    16561 gtggcaatcc aaattttata cattttcacc tatactctaa gctttcgtgt ataaaaaacg
    16621 gatataccac ttttttagta tataaacatt tttagttttt cttattaatt ttcactataa
    16681 attctagctt cttcttctcc atttagtact cttaaagttc caaaagcaag tgattccaac
    16741 tcattttctc ctgccataat ttctactggt gctataaact caactttctt tttaaccatt
    16801 gaagtaaaca tttcagaata tgcaattcca cctgttataa ttatagcatc tacctttcca
    16861 tctactacag ttgctaactc tccaatacct tttgctaact gataagccat tgcttcataa
    16921 ataatttttg cttcttcatt tccttctgct atcatttttt caacttctct agcatctaca
    16981 gtatttaggt atgaaactat accaccttta cctcttatct ttttagtcat ttctctttct
    17041 gaatattgac ctgaataaca ggcagccact aattttgtag caggtactct tcctgacctt
    17101 tctggagaaa atggtccttc atcatcagat agcatatcaa ccattcttcc tttttcatga
    17161 agataaatac ttactcctcc accaatgtga gctactatta agtttaagct cttataatct
    17221 tttcctttat ccttagcata ttttaaagcc attgctctag tatttaatgc atgccctgca
    17281 cttgatctat ccattccaca aagtccagat attctcgcta catctataag ctcatctact
    17341 gcaacagaat cataaatata tgagttgatt ccaagaggtt ctgatattgc atgtgccaca
    17401 acagcaccta aattggaagc gtgttcaagt actggtctat gtcttagtac atcaatcatt
    17461 tcttcattta ctaaatatgc tcctgatttt actggtggta aaactccacc tcttcctact
    17521 attgcactaa gagattttaa gtctatacta ttttctttaa gaatattcaa aactgcttcc
    17581 ttacgcattt caaactggtc ttgtatagta ttatattttg caatctcttc agctggatgg
    17641 tctatcgtct tcacaagaat ttgttcttct ccatcatata ctgctatttt tgtagaagta
    17701 gaacctggat ttatggctaa tattctgtaa gtcataattt tcttcctttc accttaattt
    17761 ctatagtcta tttatatcta ttataaacta tttgctaacc atttccatac cttcatggaa
    17821 agcttttatg tttaattcaa caaattttgg tttaacattt tcacttataa ttttttccca
    17881 atcaatagac tctaattgca tagatttaac tagacatcct aataaaataa cattcattat
    17941 ttttgaattt cctagttctt cagctttatc agctgcattt ataacttttg catttaatct
    18001 acttaactct gcatctatat cactttcttg atattcagct cctccgatta aaacagtcat
    18061 agaatctatc ctatgattat tcactaaagc ttttccttct ggttttaaat aattgaacca
    18121 tcttaaagct tccatttttt caaaagatac taatatatct gctccaccaa tttctataac
    18181 tggagaatga acacaatctc catatctaac ttgtgaggaa actgaacctc ctctttgact
    18241 cattccatgt atttcactca tctttacatc atatccagct tccatcaatc ccatagttaa
    18301 taatttacta gcaagtatag ttccttgacc tcctacacct accaaaagta tactcttagt
    18361 catattattt ttcctcctta actattgctt gttttggaca aacttgtgca catactccac
    18421 atcctacaca ctgatttcta tctatatttg ataacttatt ctctttatca aatgatagag
    18481 ctggacatcc agttttcaaa cataatttac atcctataca tttatcatgg tcaactttac
    18541 acttagtctt aaatggatta ttaaattctt ctatatcttc tttagagaat tttttaagta
    18601 cacatggcca tcttgttatt attactgatg gttcatcttc tatagctaaa gcccaatcta
    18661 gtgcttcatt tacttctttt aaattattag ggtttataac tcttacatgt tctattccac
    18721 aagctttaac taatccttct atatcaactt ctttagtttt agcaccttgt aaagtatatc
    18781 ctgaacctgg gttttcttgg tgacctgtca ttccagttat tctattatct aatataaccg
    18841 aaatagaatt acctctattg taaacaacat ctaaaagtcc atttattcct gtatggaaga
    18901 atgttgagtc tccaagtaca cctaccaatc ttttttcatt atcatccttc atattaagaa
    18961 ctttttgagc tccatgtgca gttccaaaag cagaacccat acaaacaaca taatctattc
    19021 cattatatgg aggagcaaat ccaagagtat aacaacctat atctccacca actattaaat
    19081 tttttctctt tccaagttca tagaagaatc ctctatgtgg acatcctgca cagaaacttg
    19141 gtggtcttgg tgttactaat tcagatttgt attctatagt atcatttgtt tttccaaata
    19201 tagcttttct taaaacatct ggtgtcattt ctccatatgg tggtattaca tcttttccaa
    19261 tacaatctat tccatatgct tttatttgtt cttctataaa tgggtcattt tcttctataa
    19321 catatatttt atcaactttt tctgcaaatt cttttatttt ttcataaggt aatggatttg
    19381 taaatcctag tttcatatat gatgcatttt ttccaaaaac ttctttggca aaattacaac
    19441 acataccaga tgctataaca cctatttttg tatcatttat ttcatagtag tttaaatcag
    19501 tttcatttga gaatttttta agaatttcca ttctttcttc aacaacgccc cttcttattt
    19561 gagcatttgc tggaacagtt accattttct tagcattttt aacatactct ttaatttcaa
    19621 cttcttctct atcatagcac tccactaatc cttttgaatg acaaagtctt gttgtaactc
    19681 tatataaaac tggtgtatcg tatttttcac taacttcaaa agcttctttt atcatatctt
    19741 tagcttcttg actagttgat ggttcaaata aaggtatctt tgcaaatttt gcatacatcc
    19801 tattgtcttg ctcattttga gatgaatgca ttcccggctc atctgctgta ataagtacca
    19861 taccaccatt tacccctgta tatgcataag taaatattgg atcagctgca acattcaaac
    19921 caacatgttt catagaagcc attgttcttg ctcctgcaat tgaaccccct atagcagcct
    19981 caagtgctac cttttcgttt ggagcccact ctgcaactat tgcatcttta tatgttgcta
    20041 tattttctaa tatttctgta cttggagtcc ctggatatgc agatgcatat ttaacaccag
    20101 cctcataggc tccacgtgca attgcttcat tacctgtcat caattgtttc atataaaatt
    20161 ccctccatct acaattatta aattcatagt ccacccaatt tgttcaatga aattaattat
    20221 ttatttttac ttctaaactt tcaagtgctt cttttataat acttggtgct ttcttacatt
    20281 tatcttcaga aactgcacaa actgcaaatc ttaagcccat ttttaatgga actgcaaata
    20341 aattatgttt agtcaattct tcacatactt cttttggatt atcacatggt atacttacaa
    20401 agaaacctgc tatatatgga agaatttcta atcctactct ttcagcttct tttacaaata
    20461 catctgctct tctagtaagc atgtttttgt atatttcttt ttcatcctca tattctttga
    20521 attttttagg gtcatttaca atgttagata atactgccat agcacttcta ttacaatttg
    20581 accaatttgc tctacatgaa tgaacacatg agtaatggaa ctcttcagct acatcttcac
    20641 ttgaagatat acaaattata gctcccattc tcataccata agctgtaaat ccttttgaca
    20701 tactgaatcc aaccaatacg agaacgtttt caggcaaatt agaaaatttc ttaaaaaact
    20761 ttctacatac atcatcatca cctgcaaagt caatatatgc tgaatctacg aaaaatatta
    20821 tctttttgtc tttatctttt gcgacttctt ttgataagtc taatatttta tcccactcat
    20881 catctgcaac actatatcca gttggattat gcgctggtgt gtttataatt gtaaagactc
    20941 taccttgttt ttctgctata tttacaaatt tttctttaaa cgactcaaaa ttaaaccttc
    21001 tattttcatc aaaaagttgg taattaacta cttttctatt agcctcttca gctatactta
    21061 catatggact ccagaaccaa tcagatgtta atacctcatc tccttcatta gtatagttcc
    21121 atacagctaa ttttattgca ccacttcctc ctggtgaagc taatactctt atatgtcctt
    21181 ctggtaagta atctctgaaa aatacttttt taactgcttc taagtaatct ggttgacctt
    21241 caagagctgc atatgcacct atctcacagt tatctaatgc tttatattct tcatatacag
    21301 ttttcattgt tatcaatttt cctgaatcat ccattagagc gcctattgtt gcatttatta
    21361 cattatccat accaaaagcc ttctctgctg cttgagctct gccagatatg ctaaatataa
    21421 catcattttc ttttggccac attgcatgct taccaaccat actttgactc ataccatcac
    21481 ccctaaaatt gtatttactc ttacattata tattaagcaa ttactatgcc aataaatccc
    21541 actttatgta aaattgattt tgtttagaat tattattttt ttcattatta aattaagtgt
    21601 tttaactatt tttcttataa atatatatgc ctgtctaaaa aaataaaaat ttgcaaaaaa
    21661 gtaatgttaa tttttcttaa cacttgtttt aaaaaattaa cattacttca aaataattta
    21721 tatgtaatta tatttaaata tttttattgt atttcttcca taaagatgtc ctgcttattc
    21781 caagaacttg tgcaacttca tttttagaaa gtccctgagc aaaaagcata cttattattt
    21841 tttcttcaac atacttattt acttctttta tatctatact tatcttacca tcatttatct
    21901 tacttatatt tatagattca tcttggactt ctacacttgc tgtcgtttca gaattaaatt
    21961 tgtctacttc agagttagga agtaattttt tagttttaaa atctctatct tgtctcaatt
    22021 taattttacc tgtcacaaca tatttttgag ctattgtctt taactctctt acatttccag
    22081 gccagctata attatatagc ttatccattt catctttagt caaaacaaaa ttttcatcta
    22141 tactgtttat tccattgaga ccatcatcct ttaatacttc atttacaaaa ttgttaaaca
    22201 aaggtattat atcttctctt ctgtctctta aaggaggtat atttatctct aaactactca
    22261 atctataaaa taagtctgct ctaaatgtac ccataactat tttttcggtt aaagattcat
    22321 tcgttgctgc tataatcctt atatccagag gtattatata atcagaacca attctcatta
    22381 tttgacgttc ttctattact cttaatagtt ttgtttgaat attaaaggat agactattta
    22441 tttcatctaa aaaaagagtc cctccatgag caagttcaaa tagtcctctt tttccaccct
    22501 ttctagcacc agtaaatgca ccttcttcat agccaaaaag ttcactttca agtaaatttt
    22561 ctgctatagt agcacaattt atagcaacaa aaggcctatc ttttctttta cttatgttgt
    22621 gtatactatg agctataatt tctttaccag acccactttc tccataaagt aaagttgtat
    22681 aatcactttt tccaattttt ttagcttctt caataaattc tttagttaat ttatctttaa
    22741 ataaaaaatc atcaaaagta tatctagcat agagaccttt ttgattcaag tcaaatctta
    22801 tttttctttc taagttttgt agcttggtta tatcttgtat tattcctaaa actccataag
    22861 tactattatc aacttttatc aatgttgtcc tagtattaac aattagatta tttatatttc
    22921 ttatctttct atcttctacg tcctcttttt catgtaaaca atctaacatc cactccattt
    22981 taggaaatac atctaagatg tatttattaa gtgcacattt tctctctact ttaagcatgt
    23041 tttttgcact ttcattgtac aaaattatac tgccattact atctatagca attactccat
    23101 cttttatacc atcaagtata tttcgtaaca cttcattatt aaacttttct tcacctaatg
    23161 tatctaataa ttttttacaa tattctacag cttctcttat agattcatca ctagcagttg
    23221 caaatacact atcaattcca tattgccttg caaaagaaca tgccaaacct cctcctacga
    23281 ttacaacttc atctttttgg tctatatatt ttacaacttt acttcttatc tcatacttag
    23341 attcaaacca ttcttcagtt atttcagtac ttatgacatt tctccagcca acatagtcaa
    23401 aagaaacttc attgtctcca agaatcaata ctactttttt agaatatttc tttgctattt
    23461 ctattgctct taaaaggtca gttgacttca tattcattgg tataacaggt acatttactg
    23521 tatctattac taagctataa cctccacctc taccaattat agcttgggca cctttttcaa
    23581 caagtatacg accttgattt tccattaagt ctaggttaag tatgtcgatt attatagttc
    23641 cattttctac atcttctcta tataattctt ctattctttc ttttaattca atatcagagg
    23701 ctataattcc tatttttttt cgcaacaaaa tcacctcaat tttaaattat aatgtattga
    23761 tttctacctg ctttttttga tttatatagg ttcttatctg catcttcaat taatatttca
    23821 acatcttttc tactacgaca cctttttata cttactccag cacttatagt tatatagttt
    23881 tttattttag aatatatatg ttcaataccc tcaaattcaa gaagtttgaa aactctttta
    23941 atatataatt ctatattatc aatctcttca acaccgattg attctataga atctactaat
    24001 attaaaattt cttctccccc atatctgatt atgtgttcgg taatatctat aaatacactt
    24061 cttataatat cagttacctt aactataact ttatcccctg ctagatgacc ataattatca
    24121 ttataacttt taaaacaatc tatatcgacc agtattgcga ccatattttt gtctctggtt
    24181 ccattaaata aatccaactg ctgttccaaa tatcttctat tatatagttt agtaagtcca
    24241 tcttttaaaa tatcattttc taatttctta ttatcatcca ctagtaatct taaactctct
    24301 tgtttttgtc tgagttcata ttgtttgtga attgcttgag atgttttata ggaattattt
    24361 ttttcccacc tatcagaata ccaatccata cttttataat attctattgc cagtttaaaa
    24421 tcttttttta gctcatatcc atttgctatt agcccatata aatatagata tttttctaag
    24481 attcaatgaa ctctttttaa ctaaatttaa accttttata taacaactta taccttcatc
    24541 tatattacca aattttatat ataaat
//
LOCUS       DAMJYR010000051        24071 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.051, whole genome shotgun sequence.
ACCESSION   DAMJYR010000051 DAMJYR010000000
VERSION     DAMJYR010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 24071)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24071)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24071
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.051"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(35..1111)
                     /locus_tag="PC471_001995"
     CDS             complement(35..1111)
                     /locus_tag="PC471_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HDF2789124.1"
                     /translation="MSKKLFIPGPIDVKEEVLQKMATPMIGHRGKDASMLQKSISEKM
                     QKLFYTNNTILLSTSSGTGLMEGSIRSCTSKKAAVFSCGSFGDRWYKMAVANNVPADI
                     FKVELGEATTPEMVDKVLSTGEYDLITVTHNETSTGIRNPIEEIGEVVKKYEDVIYCV
                     DTVSSAGGIKVEVDKIGIDICITSVQKALGLPPGMSICTFSQKAIDRAKQVPFRGVYL
                     DLLAMYEYLIKKNYQYPSTPSLSHMFALDFQLDNILDEGLDNRFNRHEDMANLVRNWA
                     KKHFQIFTNENHLSNTLTVIENTQGISVSNLNSKLQERGFQIANGYGDLKEKTFRISH
                     MGDYTVEDVQELLDNIDDILGFNK"
     gene            complement(1484..1999)
                     /locus_tag="PC471_001996"
     CDS             complement(1484..1999)
                     /locus_tag="PC471_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HDF2789125.1"
                     /translation="MDFLAKYKVLKKQNSSKSCLVCGTQNELGLKADFYELENGELVS
                     ICNTKDWHQSYPGRVHGGMSAAILDETIGRAVSINDDQIWGVTVSLELKYKKPVPTDA
                     TIKVVGRITKENRKLFEGTGEIILPNGDIAVTATGKYMKMPIGQIAEGDFSNEEWFFE
                     ESKEKVEYIEL"
     gene            complement(2362..3432)
                     /gene="leuB"
                     /locus_tag="PC471_001997"
     CDS             complement(2362..3432)
                     /gene="leuB"
                     /locus_tag="PC471_001997"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HDF2789126.1"
                     /translation="MNCNIAVIKGDGVGPEIIDEGIKVLNKICCKFNHRFDCEYVLAG
                     GCAIDETGEPLPNKTVETCRKNEAVLLGAVGGPKWDKCKGDKRPESGLLKLRESLGLF
                     ANLRPATMYESIKEASPLRTDIVEKGIDFVVVRELTGGIYFGERGRKIIDGIENAYDV
                     EIYNENEIRRIGKRAFEIARNRNKKLISVDKANVLESSRLWRSIMEDLAKEFEDVELS
                     HMYVDNAAMQVVKDPSQFDVIVTNNIFGDIISDEASMITGSIGMLPSASLREDSFGMY
                     EPIHGSAPDIAGKDIVNPIATILSVSMMLRHSFNLEEEAKCIEDAVQSVLNKGYRTID
                     IYNGVGNVVGTREMGELIVNEI"
     gene            complement(3439..3930)
                     /gene="leuD"
                     /locus_tag="PC471_001998"
     CDS             complement(3439..3930)
                     /gene="leuD"
                     /locus_tag="PC471_001998"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HDF2789127.1"
                     /translation="MIANGSVFKFGDNIDTDVIIPARYLNIADYKELATHCMEDIDDK
                     FISKVKKGDIIVATKNFGCGSSREHAPIVIKESGVSCVIASTFARIFFRNSINIGLPI
                     LECEEAANNIDEGDNIEVDFSTGVIKNITKGKEYKAEPFPEFMQNIILNEGLINSIKA
                     NRG"
     gene            complement(3945..5222)
                     /gene="leuC"
                     /locus_tag="PC471_001999"
     CDS             complement(3945..5222)
                     /gene="leuC"
                     /locus_tag="PC471_001999"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HDF2789128.1"
                     /translation="MGMTMTQKILAKHAKLNEVKKGQLIEADLDVVLGNDITSPVAIR
                     EFEKLGIEDVYDKTKVVMVLDHFTPNKDIKSAEQCKFTRSFAKSKGVVNFFDVGDMGI
                     EHVLLPEKGIVTAGDVIIGADSHTCTYGALGAFSTGVGSTDMGAGMATGKCWFKVPGA
                     IKFVLKNKPNKWISGKDIILHIIGEIGVDGALYKSMEFCGDGVEYLSMDDRFTICNMA
                     IEAGAKNGIFPVDDKTMEYINSHKCSTMTKDVNIYEADEDAVYDEVYEIDLAELKETV
                     AFPHLPENTRTVDEIDKDIKIDQVVIGSCTNGRISDLEVVAEIMKGKKVADGVRVMIF
                     PGTQKVYLEAIEKGYITTFIEAGAAVSTPTCGPCLGGHMGILAAGEKSISTTNRNFVG
                     RMGHVDSEVYLASPAVAAASAITGKISKPSEII"
     gene            complement(5179..6921)
                     /gene="leuA"
                     /locus_tag="PC471_002000"
     CDS             complement(5179..6921)
                     /gene="leuA"
                     /locus_tag="PC471_002000"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HDF2789129.1"
                     /translation="MKCGKYKKYDKMQIVNRKWPDNEIFKAPIWCSVDLRDGNQSLPT
                     PMSVNEKVRMFKMLIDTGFKEIEVGFPSASNTEYTFLRKLIDENMIPDDVTIQVLTQS
                     RAHLIEKTFESIRGCKKAIIHLYNSTSVLQRDVVFNMSKQEIIDIAVEGAKLFNEEVK
                     KYPETEFTFEYSPESFTGTEMDYALEICEAVIDVWKPTTQKKVIINLPSTVEMATPNV
                     YADQIEWFCKNISCRDSIILSLHTHNDRGTCTAASELGLLAGADRLEGTLFGNGERTG
                     NMDIVNVGLNLYTQGIDPELDFSNIDKIIGIYEDCTKLMVHDRHPYAGNLVHCAFSGS
                     HQDAIRKGMIAMKNRDNDYWEVPYLPIDPHDIGREYKEIIRINSQSGKGGAVYIMETD
                     YGFMIPKNMHSDFGNVVKMESDRIGEELSSEAIFNLFKKEYIEVESPYKVKKYKIKSM
                     DELNYENDDSNDTNMIEMTARISYMGNEQRIVGIGNGPVDSFNNALKQCGMKDYKFRY
                     YWEHALEEGSHSRGVAYVGIEHNNEVYFGVSISENINTAAINALMNAINKSYIEEEIK
                     NGDDYDAENISQTC"
     misc_binding    complement(7045..7291)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(7439..7885)
                     /locus_tag="PC471_002001"
     CDS             complement(7439..7885)
                     /locus_tag="PC471_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2789130.1"
                     /translation="MSKNKFIYMNYYDNGYFKKGMTIYMVLALIILATIFIYSTTILY
                     ILCIITAVFLFKQGRDFSNKYEGKVYITLDNHSILINNQCIFSIKSKQSQKFNYKIIE
                     KIEVVKNILNIYTDESNYKIRLRALSLEDEKKLLNIIDEKMKKFKA"
     gene            complement(7906..8625)
                     /locus_tag="PC471_002002"
     CDS             complement(7906..8625)
                     /locus_tag="PC471_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase"
                     /protein_id="HDF2789131.1"
                     /translation="MKKSIMDVHCHTLISGHAHSTFKENVEEASKKNIKYLGISDHGP
                     NMPGGPHPFYFYNLHLLPREVQGVKILRGIEGNIMDYNGNLDVQEDMLQHLDYIIASL
                     HRPCIASGTKEENTNAILKVMDKPKVKIIGHPDDSRYPLDYEPIVKKAKDKNILLEIN
                     NSSLSSNSHRTGTWENVSHMLTLCKTYGARVILGTDSHICYSIGEFENAEKVLEAVDF
                     PDELVINYHEDEIIEFFDINF"
     gene            complement(8743..10017)
                     /locus_tag="PC471_002003"
     CDS             complement(8743..10017)
                     /locus_tag="PC471_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HDF2789132.1"
                     /translation="MFIMENDLTMQELLDQQEQVFSKVKVGELTTGKITAVRNDEVQL
                     GLDYGFDGIIPISELNIEKNQYIEDIYHIGDEITAVITKVSQKDGTITLSKLQLDKRN
                     DFAELQKAYDEHRIITVNVEKNIDKGVFANYNTYTFFIPISQLDTKFITDTSKFVGLN
                     LEVYIKELDVRKNRLVASHRDVLQERINKEREERRAQIKAEKEAERARIKQEREEEKA
                     RIKAAKEDLFNSLEVGQKRDGKVTKIMPYGAFVDIGGIEGLAHINNLAWTRVESVEDV
                     VSEGQEVEVYVLDVNKETKKIALALKDINNDPWDLIAKEVQIDDVVNVKVLRIIEKGA
                     FVQIKEGVDAYLPISELSDTRVAKVTNVVNIGDEVKVKILDFKPKTKRMLVSIKEATR
                     EPEEDITEYLEVEESLGSIGELFKDKFKDLEV"
     gene            complement(10217..11584)
                     /locus_tag="PC471_002004"
     CDS             complement(10217..11584)
                     /locus_tag="PC471_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HDF2789133.1"
                     /translation="MENSNTNLGSESVGKLLFKLATPAIIAQIVNVLYNIVDRIFIGR
                     MENGEVAMAGVGVAFPIIIIITACSYLIGMGGGPLAAIKMGEQNNDEAEKIMSNSFSV
                     LVILAILLTIGFKIGKEPLLWMFGASESTIGYSMDYLNIYLIGTVFVQISMGMNTFIN
                     TQGFATTGMMTVAIGALINIILDPIFIFGFNMGVKGAALATIIAQGVSAIWVLMFLFG
                     KKSILKIKKKYMIPKASIILPVLGLGISPFIMQSTESLVLIALNSKLQMYGGDLAVGS
                     MAIMSSIMQILMLPNMGVTQGAQPIISYNYGSGQLDRVKKTFKLCLLSCFTYSTILWL
                     LLMIFPAFFVSIFNKNPQLLSMTSWSIKIYFAGAFMFGIQIACQQTFLALGKATISLV
                     LALLRKIVLLIPLIFILPTFFNEKLFAVILAEPVADITAATITAISFFIFYKCFLSKP
                     KAIKE"
     gene            complement(11834..12298)
                     /locus_tag="PC471_002005"
     CDS             complement(11834..12298)
                     /locus_tag="PC471_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small multi-drug export protein"
                     /protein_id="HDF2789134.1"
                     /translation="MSLRIGGNSLGYLHILILSMVPVTELRGAIPIGIAMGLNPIWVY
                     VFSVIGSTLVSIPLILTFRHVLQFLRGKKLFKGIADVIDRKINSRMKKLKSVSIIGII
                     LFVGIPLPTTGTWTASAIASILKMRIKDAFMGVFLGNLLSGVIVSALSLHII"
     gene            complement(12303..12932)
                     /locus_tag="PC471_002006"
     CDS             complement(12303..12932)
                     /locus_tag="PC471_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family hydrolase"
                     /protein_id="HDF2789135.1"
                     /translation="MKKSVLLLDVDYTVINTDSMIDFFIYSLKNKTFKTIIKLPYIIF
                     ILFMYLIRMIPLKKAKEAIFYPIVDFSEEDLKKFFDDCIMKKINESMKKVIYKNKEED
                     NVIIMITASPYAYMKYFKYYGLADEVIGTEFFYENSRYKNQFIGENCKGIEKVKRIKA
                     VLGKLGIEIDYENSYAYSDSKSDLPMLSLTKNAFLVSKKDGEVIEQINS"
     gene            complement(12966..13853)
                     /locus_tag="PC471_002007"
     CDS             complement(12966..13853)
                     /locus_tag="PC471_002007"
                     /EC_number="2.4.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decaprenyl-phosphate phosphoribosyltransferase"
                     /protein_id="HDF2789136.1"
                     /translation="MKQTNTSIRLLTKISDYIKLMRIKQYIKNFFVFSAIIFSNNILN
                     INLFLNTFIAFVCFCLMSSSVYALNDAIDMDKDKKHPKKCNRPVASGRISKKSANILF
                     VVLALISVCLSTVVSFNLSIILSLYLINNILYSLKLKNIILLDVFSISLGFILRVYAG
                     CVAISVSLSNWIILCTFFLSLYLALGKRKKEIETLRDDAVEHRKILEDYDIENLNQMM
                     IVILSSTIVCYALYSTSNPEKPHMIFTTIFVVYGVLRYNYIINTTNENNPTDIVLKDN
                     ALKINVILWIITCLIILIF"
     gene            complement(13882..15120)
                     /locus_tag="PC471_002008"
     CDS             complement(13882..15120)
                     /locus_tag="PC471_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2789137.1"
                     /translation="MDGDILMSKILKYKNEMFLFILVVITYLIITKIFFSKTTIFYDL
                     NNTYDVLLDTDTGVLFNLNVFAISQDNSKHILFSAIISIFAYPIYLFCTSIANPGTTD
                     FNSAYGFGLICLQIITSAMSITLVFNHIKKIKMQRLTLILLTMIMIFSFPQLFMTLNV
                     ERFIYSQFSLIFFIVIANKMKGKNSYLVELAAIPLFGITISNIYLYFFNMIFEFKLKI
                     GKMLKHLITFILMAYICVVSTKSYESFMNLGNVIQYDTKFISGEPILEKIAMIIERLL
                     YSVFYFPGASIKKGLFLQNGEVATIPVILTLLALCFCVLSVIENSEKRVPKLCMGIII
                     FNLTLHGIVGYNLVNSSIMAINFSFAVIILLAYFTKALRKNEKNMYNIFLSLLLVTIV
                     ISNVNGFIEILNIGIKSYPV"
     gene            complement(15504..16646)
                     /locus_tag="PC471_002009"
     CDS             complement(15504..16646)
                     /locus_tag="PC471_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HDF2789138.1"
                     /translation="MRYVEYGKTGKMVSVVGYGGLRFDLEKSNQENADLVKYAYEKGI
                     NYFDTAPGYCDDRSEDIFGLAFRQMIKEGKTDFYVSTKEKPKACDTSEKAIEAVKKSI
                     ERLGVSKINFYHVWNIRKMEHYELAMRPGGQYEGLLKCKEEGLIDHIVFSSHQPGDEV
                     IDILSENKFEGVTMGINILNFPYRLKGAKYAVDNGYGVVAMNPLSGGTIPKYNKELSF
                     LSKDGESTIESALRFNIGISQISISLIGFNKKQDIDDACKIADENRIYSDEDIHDIET
                     RLNKNMNEICTGCGYCKVCPKGINTPAYMLFYNEKQMFKKSDEEMTKLVYGLGHWNYT
                     MNSKAKAKECISCGKCEVECTQHLPIIDRLKEIKKWEEDGANSVKV"
     gene            complement(16787..17653)
                     /locus_tag="PC471_002010"
     CDS             complement(16787..17653)
                     /locus_tag="PC471_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HDF2789139.1"
                     /translation="MDNYKKVKQVLWIILFANFAVALLKIIIGNQIKSYSMTADGFHS
                     LSDGASNIVGLIGIFFASKPKDKNHPYGHKKFEIITSLFISGMLFVIAIKIILSAVLR
                     IANPVVPAITIESLIALIITLFINIFVCMYEYRIGTKLNSYVLISDSLHTRSDIFVSL
                     GVLVTLVGVKLGFPVIIESIVPIIISAFIIYSAYGIFRPSIGILVDRVAVDEDYIKEI
                     VFEFNEVRDVHNIRSRGSKSSIYIDMHVMVDPFISVEQSHDLTHKIEKQIQEEINENA
                     QVIVHIEPFYSF"
     gene            complement(17988..19535)
                     /locus_tag="PC471_002011"
     CDS             complement(17988..19535)
                     /locus_tag="PC471_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HDF2789140.1"
                     /translation="MANFFNFILLQKDKIIELLIQHMSLTVTSILIAIIVGVPLGIII
                     SRISSLRKFVLGFVNLVQAVPSMALLGLLVPILGIGSKPAIFMVVVYSLLPIVKNTYI
                     GITSIDPVVLESAKGIGLTRNQTLFKIQFPLALPIIMGGVRISAVTAVGLMTLAAFIG
                     AGGLGYLVFSGVQTVNNNMILAGAIPSCIIALLVDYLFSKIEVAVTPKGLNPKAPKKN
                     YIALKVISVVVVVSMLFVVFSSSFSSKKDTITIGSKDYTEQLILGNVYAELVEKNTNL
                     KVKKNLNLGGSSVAFNAIKSGELDMYVDYTGTLLVNVMKHAPIKDADEAYNVVKDTME
                     KENQLTLLDPLGFNNTYTLAMMPETAEKYGINTISDLTKYGKEFTFSPTLEFENREDG
                     LVGLSRDYGLKFKDVKAMNGSLRYTALDNNESQVIDAFLTDGLIKKFNLKILEDDKNF
                     FVPYYAAPLVREDTLKKYPELEKVMNMLSGKINEETMRELNYQVDELGKSPEEVAHSF
                     LVKEGLV"
     gene            complement(19528..20664)
                     /locus_tag="PC471_002012"
     CDS             complement(19528..20664)
                     /locus_tag="PC471_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2789141.1"
                     /translation="MIEIRNVTKKIGNNVILDDISLVVETGTLVVLIGSSGCGKTTTL
                     KLINKLIKPTSGEIYINGKPISQENEIELRRKIGYVIQNTGLFPHLTIKENIELIPRL
                     KKEKSVEEIEKRTLQLLEMVGLDSDEFLNKYPSELSGGQQQRIGVARAIATDAEIILM
                     DEPFSALDPITRTSLQEQLFSLQDELKKTIIFVTHDMDEALKIADKICIMKDGRIAQY
                     DTPENILRKPANDFVKDFIGEDRVWDNPEYIKARDIMIKNPIAVNSTRTVTQGIEIMR
                     TSKVDSLLIIDRAKTLKGIVTVKDMKDIDDKSILLADIMSSEPLHVNEGDNLVEILNV
                     MNRNSVGYIPVISDENKLVGLITRSSLLSVLSEQFLEMEVSVLG"
     gene            complement(20927..22024)
                     /gene="dinB"
                     /locus_tag="PC471_002013"
     CDS             complement(20927..22024)
                     /gene="dinB"
                     /locus_tag="PC471_002013"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HDF2789142.1"
                     /translation="MKHYRKIIHIDMDAFYASIEQRDNKKLKGRPVIVGGNPQSRGVV
                     ATCSYEARKFGIHSAMPSAVAYNRCPYAVFVRPRFDVYKSVSEQIRDIFYRYTDLVEP
                     LSLDEAYLDVTKNKKNIDSSIEIAKQIKKDIFREVGLTSSAGVSYNKFLAKIASDLRK
                     PNGLTVITEENAQDFLDKLPVNKFFGVGKVTSNTLKNLGIKTGYDLRCLNLFELENIF
                     KKRGYELYKFARGIDDRPVEPNRVRKSVGAETTLSHNLDIDEEETRNILDELCEEVCH
                     RLKNSEKFGKTLTLKIKYEDFTKITRSLSLEHYIDEYNDIRSGVDNLLRNVEVNGKQI
                     RLLGVTISNLSDKKETYKDITLFEYMDSIQM"
     gene            complement(22414..23919)
                     /locus_tag="PC471_002014"
     CDS             complement(22414..23919)
                     /locus_tag="PC471_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003374392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HDF2789143.1"
                     /translation="MKVGFDHKKYLEEQSKYILERVNNFDKLYLEFGGKLMGDLHAKR
                     VLPGFDENAKIKVLQHMKEKVEVVICVYAGDIERNKIRGDFGITYDMEVLRLIDDLRG
                     YELDVNSVVITRFEGQPATTVFINKLERRGIKVYKHYPTKGYPSDVETIVSDEGYGAN
                     EYIETTKPIVVVTAPGPNSGKLGTCLSQLYHENKRGNVVGYSKFETFPVWNVPVKHPL
                     NIAYEAATVDLKDVNMIDSFHLETYGEMSVNYNRDLELFPVLKKIIEKITGKESIFQS
                     PTDMGVNRVGFGIIDDEVIRKASIEEIIRRYFKTACEYKKGQVDKGAYDRMKMIMEEL
                     NLKPEDRNVVLPARNYSNKLKESADKNDTCPVVALELEDGTILTGKGSDLMNGTAAVV
                     LNAIKHLANISDDMHLISPVILEPIINLKTKTLASRNVSLSCQEVLTALSICAVTNPT
                     AQFAMEKLALLKGCQAHSTTILNRDDEQTFRKLGIDVTCDPEYPSQNLYYS"
BASE COUNT         7873 a         4348 c         2532 g         9318 t
ORIGIN      
        1 aaatttatct taaaacatac atatttttac aatattattt attaaaacct aatatatcat
       61 caatattatc taataattct tgtacatctt cgacagtata gtcacccata tgtgaaattc
      121 taaaagtttt ttcttttaaa tctccatagc catttgctat ttggaatcct cgttcttgta
      181 attttgaatt taaatttgat acactaatac cttgagtatt ttctataaca gtaagtgtat
      241 tggataagtg attttcattg gtaaaaattt ggaagtgttt tttagcccag tttctaacta
      301 agtttgccat gtcttcatgt ctgttgaatc tgttatctag gccttcatct aatatattat
      361 ctaattggaa atctaaagcg aacatgtgag aaagagatgg tgttgaaggg tattggtagt
      421 ttttcttgat taagtattca tacattgcta acaaatcaag atatactcct ctaaaaggta
      481 cttgcttagc tctatcgatt gctttttgag aaaatgtgca aatagacatt cctggtggta
      541 atcccagtgc tttttgtact gatgttatac aaatatctat tccaatctta tcaacctcga
      601 cttttattcc accggctgaa cttacagtat ctacacagta gattacatcc tcatactttt
      661 taacaacttc tccaatttct tctattggat ttcttattcc agttgaagtt tcattgtgag
      721 ttacagttat taaatcgtat tctccggtag aaagcacctt atcaaccatt tctggcgttg
      781 ttgcctctcc taattcaact ttaaatatat ccgctgggac attattagcc actgccattt
      841 tgtaccatct atcaccaaag gatccacagg aaaatactgc tgcctttttg gatgtacagg
      901 aacgaatgga gccttccatt aaaccagttc cggatgacgt ggataaaagt atcgtgttgt
      961 ttgtatagaa tagtttttgc atcttttcac ttatactctt ttggagcata gatgcatctt
     1021 ttcctctatg tcctatcatt ggtgtagcca tcttttgcaa gacttcttct ttaacatcaa
     1081 taggaccagg aataaataat ttcttactca ttataattcc ccccttttta gtttgtaata
     1141 tggatagcgt ttttactgtc cttattttta ataactataa tatcaagctg aattaactat
     1201 gtcaacataa aatttatttt ttatatataa tttattttat atattttatg aaaacattca
     1261 ttttctacct tttcataagt ctgaattttc tgaattatcc cctcaaaaaa aatatacttt
     1321 ttatctatat aaattaattt ttttagtatg taaattttac gctattacga taaatctttt
     1381 tagtataact aatttatttt tatcttgtat ttttattgta tttttgaatt ttattttttc
     1441 taaatataaa atttatgttt taaaatataa aatttttatt taatcaaagt tcaatatatt
     1501 ccactttttc tttcgattct tcaaaaaacc attcttcatt agaaaaatca ccttctgcaa
     1561 tttgtccaat aggcattttc atatactttc cagttgctgt cactgctata tctccatttg
     1621 gtagaattat ttctcctgta ccttcaaata attttctgtt ttctttagtt attcttccaa
     1681 caacttttat agtagcatct gttggtactg gtttcttata tttcaactct aaagatactg
     1741 ttactcccca tatttggtca tcatttatac ttacagctct tccaatagtc tcatccaata
     1801 ttgcagcaga catacctcca tgaactctcc caggataact ttgatgccaa tcctttgtat
     1861 tacaaattga tacaagctct ccattttcaa gttcataaaa atctgcttta agtcctagct
     1921 cattttgtgt tccacaaact aaacaagatt tactactatt ttgcttcttc aaaactttat
     1981 actttgccaa aaaatccatc tcctaaccga cattagtagt cgcttaatat taaataattc
     2041 taaaatatat tatataatat taataaaatc cctgcaaact tctaataata aaaataggct
     2101 acttcaatat gataagttta atttaaagta ttattttcca aaaaataact ttatcaaaat
     2161 agaaactcaa agtagatata aacctctttt tcgttattaa aatagtaaaa atatatttga
     2221 cttaatataa aaaagagtgt ctcattaact aataaagttt attttgagac acccctcata
     2281 aaacaaatta tactacaata gatttttact aattctacaa gttatatatt taaattatat
     2341 tattcaaatt aaactataat tctaaatttc atttactatt aattcgccca tttcccttgt
     2401 tcctacaaca tttcccacac cattatagat atcaatagtt ctataacctt tattgagtac
     2461 actttgtaca gcatcttcaa tacacttagc ttcttcttct aagttaaaac tatgtcttaa
     2521 catcatgctt acagatagaa tagtcgcaat tggatttaca atatcttttc cagctatatc
     2581 aggtgcactt ccatgtattg gttcatacat accaaaacta tcttctctaa gtgatgctga
     2641 tggtaacata cctatagaac ccgtaatcat agatgcttca tcagatatta tatctccaaa
     2701 tatattatta gtcactatta catcaaattg gctagggtct tttacaactt gcatagcagc
     2761 attatcaaca tacatatgtg acagttctac atcttcaaac tctttagcta agtcttccat
     2821 tatacttctc caaagtcttg aactctcaag cacatttgcc ttatctacac ttataagttt
     2881 tttatttcta tttctggcta tttcaaaagc tctcttacca attcttctta tttcattttc
     2941 gttatatatt tctacatcat aagcattttc gataccatca atgatttttc ttccacgttc
     3001 tccaaaataa atgccccctg ttaattccct tacaacaaca aaatcaatgc ctttctcaac
     3061 tatatccgtt ctaagtggtg aagcttcttt tatactttca tacattgtag ctggtcttaa
     3121 atttgcaaat aaacccaatg actctcttaa ttttaaaaga ccagactctg gtcttttatc
     3181 tcccttacat ttatcccact ttggtcctcc aacagcacca agcaatacag cttcgttttt
     3241 tcgacaagtt tcaacagttt tattaggtag aggttctcca gtttcatcaa tcgcgcatcc
     3301 tccagctaat acatattcac aatcgaatct atgattaaat ttacaacaaa ttttatttag
     3361 aacctttata ccttcatcta taatttccgg tccaacacca tcacctttta tgactgctat
     3421 attacaattc atacttcact accccctatt tgcttttatt gagttaatta agccttcatt
     3481 taatataata ttttgcataa actcaggaaa tggttctgct ttatattctt ttccttttgt
     3541 tatatttttt atcacacctg tagaaaaatc tacttctata ttatcccctt catctatgtt
     3601 attggcagct tcttcacatt caagtatagg aagccctata tttatagagt ttctaaaaaa
     3661 tattctagca aaagtagaag caatcacaca ggatactcca ctttctttta ttacaattgg
     3721 tgcatgttct cttgatgaac cacaaccaaa gtttttagtt gcaactataa tatctccttt
     3781 ttttacctta gaaataaatt tatcatcaat atcctccata caatgcgttg ctagttcttt
     3841 ataatcagct atatttaaat atctagctgg tattattaca tctgtatcta tattgtcacc
     3901 gaatttaaaa acacttccat tggcaatcat aaattttacc tcccttaaat tatttctgat
     3961 ggttttgata tttttccagt tatagctgat gctgctgcta cagctgggct tgcaagatac
     4021 acctctgaat caacatgccc cattcttcct acgaaattcc tatttgttgt tgaaatggat
     4081 ttttctcctg ctgcaagtat acccatatgt ccccctagac atggaccaca agttggtgta
     4141 gaaactgctg ctcctgcctc tataaaagtg gtaatatacc ctttttctat agcttcaaga
     4201 tatacttttt gagttccagg aaatatcata actctaactc catctgctac ttttttgcct
     4261 ttcattatct cagctactac ttcaaggtct gatattcttc catttgtaca agagcctatt
     4321 acaacttggt ctatctttat atccttatct atctcatcta ctgttctagt attttcagga
     4381 agatgaggaa aggctactgt ttcttttagt tcagctaaat ctatttcgta tacttcatca
     4441 tagactgcat cttcatctgc ttcatatata tttacatctt ttgtcatagt tgaacactta
     4501 tgtgaattta tatattccat agtcttatcg tctactggaa aaattccatt ttttgctcct
     4561 gcttctattg ccatattaca aatagtaaat ctatcatcca tagataagta ttctactcca
     4621 tctccacaaa attccattga cttgtaaagt gctccatcaa cacctatctc ccctattata
     4681 tgaagtatta tatctttacc acttatccat ttatttggtt tattcttcaa aacaaatttt
     4741 attgctccag gaaccttaaa ccaacatttt cctgtagcca tacctgctcc catatcagtt
     4801 gaacctacac cagtagaaaa tgctcctaat gctccatatg tgcaagtatg agagtcagca
     4861 cctataatta catctccagc agttactata cctttttcag gaagaagaac atgctctatt
     4921 cccatatctc ctacatcaaa aaaatttaca actccttttg attttgcgaa acttcttgta
     4981 aatttacatt gttctgcact ctttatatct ttattaggtg taaagtggtc taaaaccatc
     5041 actactttag tcttatcata aacatcttca attcctaatt tttcaaattc tctaatggct
     5101 actggtgatg ttatatcatt acctaaaact acatctaaat ctgcttcgat taattgccct
     5161 ttcttaactt catttaattt agcatgtttg gctaatattt tctgcgtcat agtcatcccc
     5221 attttttatt tcctcctcaa tataactttt atttattgca ttcataagtg cattaatagc
     5281 tgctgtattt atgttttccg atatacttac accaaaatag acttcattat tgtgctctat
     5341 tcctacataa gctacccctc ttgaatgtga cccttcctct aatgcatgtt cccagtaata
     5401 tctaaattta taatctttca tgccacattg ctttagtgca ttattaaatg aatctacagg
     5461 tccatttcct ataccaacaa tcctttgttc atttcccata taacttattc ttgcagtcat
     5521 ctcaatcatg ttagtatcat tagaatcatc attttcatag tttaattcat ccattgattt
     5581 tattttatac ttcttaactt tataagggct ctcaacttca atatattctt tcttaaacaa
     5641 attaaatatt gcttcactac taagttcttc cccaattcta tcagattcca ttttgactac
     5701 atttccaaaa tcagaatgca tattcttagg tatcataaat ccataatctg tttccattat
     5761 ataaacagct cctccttttc cagattggct atttatcctt ataatttctt tgtattctct
     5821 tccaatatca tgagggtcta ttggtaaata tggaacttcc cagtaatcat tatctctgtt
     5881 tttcattgct atcatgcctt ttctaatagc atcttgatgt gaacctgaaa aagcacaatg
     5941 tacaagatta cctgcatatg gatgtctatc atgtaccata agttttgtac aatcttcata
     6001 tatacctatt atcttatcta tattgctaaa atctagctct gggtctattc cttgtgtata
     6061 aagattaagt cccacattta ctatatccat attcccagtt ctttcaccat ttccaaatag
     6121 tgttccttca agtctatcgg caccagccaa taatccaagt tcacttgcag ccgtacaagt
     6181 tcctctatca ttgtgagtat gcaaacttag tataatagaa tccctacaac ttatattttt
     6241 acaaaaccat tctatttggt ctgcatatac gtttggtgtt gccatttcaa ctgttgatgg
     6301 aagatttatt attacttttt tttgagtagt tggtttccaa acatctatca ctgcctcaca
     6361 tatttcaagt gcataatcca tctctgttcc tgtaaaactc tctggtgaat attcaaaagt
     6421 aaattctgtt tctggatatt tttttacttc ttcattaaat aattttgctc cttcaactgc
     6481 tatatctatt atttcttgtt tactcatatt aaaaaccaca tctctttgaa gaacagatgt
     6541 agagttatat aaatgtataa ttgctttttt acatcccctt attgattcaa atgttttttc
     6601 tataagatga gccctagatt gtgtaagtac ttgtatagtt acatcatctg gaatcatatt
     6661 ctcatctatt aattttctta aaaatgtata ctctgtattt gatgctgatg gaaatcctac
     6721 ctctatttct ttaaatcctg tatcaataag cattttgaac attctgactt tttcatttac
     6781 actcataggt gttggcagtg actgatttcc gtctcttaaa tcgacactac accaaattgg
     6841 agccttaaat atttcattat ctggccattt acgatttact atttgcattt tatcatattt
     6901 tttatacttt ccacacttca tgttatttcc ccctttttct gttttttgta ttcagtatac
     6961 ctcatatcga cgcaatcctg cacaattcat aaggataaat gtcaatttta tcattagaat
     7021 aaaaaaaacc tcatctctaa taaaattaga gacgaggttt tgctcgcggt accactctat
     7081 ttagctttct aaaaagctcc cttagtaata gccaatacaa taattataat gactatattt
     7141 cctataacgt ggaattactc cgattattct aagatttaaa tcttagaaaa cagctcctag
     7201 acaagttcag aactatcaac tactaaatca caccatcctt tagctctctg aaagtttcat
     7261 tgttcttact acttctattc atcacttttt taatcattta aattttctaa atattttgat
     7321 ttaatagtta ttatatacac attaaaatgt attgtcaaca caaaaaataa ttatctcaat
     7381 tttttatttt tgataagcaa ttctataaaa catgctaact attgaaaaca gtatgatatt
     7441 atgccttaaa ctttttcatc ttttcatcta tgatattcaa taattttttt tcatcttcca
     7501 aactcaatgc ccttaatcta attttataat tagattcgtc agtgtatata tttaatatat
     7561 tttttactac ttctattttt tctattatct tataattaaa cttttgactt tgtttagatt
     7621 ttatactgaa tatacattga ttatttatta atattgaatg attatcgaga gtaatatata
     7681 cttttccctc atatttattt gaaaaatcac gaccttgttt aaatagaaaa actgctgtta
     7741 ttatacataa aatatataat attgtcgttg aatatataaa aattgtagct aaaattataa
     7801 gtgctaaaac catatatata gtcattcctt ttttaaaata accattgtca taataattca
     7861 tatagataaa cttattttta ctcaaatttt ttcacttcca agattctaaa aatttatatc
     7921 aaaaaattct ataatttcat cttcatgata atttatgact aattcatctg gaaaatctac
     7981 tgcttctaaa actttttctg cattttcaaa ttctcctata gaataacata tatgagagtc
     8041 agtaccaaga attactcttg caccatatgt tttacacaat gtcaacatat gacttacatt
     8101 ttcccaagta cccgttctat gagagtttga acttaatgat gaattattta tttctaaaag
     8161 gatattttta tctttagctt tttttactat aggttcataa tctaaaggat acctactgtc
     8221 atctggatgt ccaattattt ttaccttagg tttatccata acttttaaaa tagcatttgt
     8281 attttcttct tttgtaccag aagctataca aggtctatgc aaacttgcga ttatataatc
     8341 caaatgttgc aacatatctt cttgaacatc taaattacca ttataatcca ttatatttcc
     8401 ttctattcct ctaagaatct ttacgccttg cacttcacgt ggaagcaaat gcaaattata
     8461 aaaatagaat ggatgtggtc ctcctggcat attaggtcca tggtctgata ttcctaagta
     8521 ttttatattc ttcttactcg cctcttcgac attttccttg aaagtactat gtgcatgtcc
     8581 acttattaga gtgtgacaat gtacatccat aatagacttt ttcattaaat ttcctcctaa
     8641 tattttacat aaattcgtga aaatagctaa agggctattt caaaactaaa gctgtcataa
     8701 tagacagtgt aacgttttaa aacagcccca ttttcaatta ttttacactt ctaaatcttt
     8761 aaatttatct ttaaataatt ctccaatact tcctaaagat tcctctactt ctaggtattc
     8821 tgttatatcc tcttctggct ctctagttgc ttcttttatt gaaaccaaca ttctcttagt
     8881 ctttggttta aaatcaagaa tcttaacttt aacctcatct ccaatattaa caacattagt
     8941 aactttagcc actctagtat cactcaattc agatattggt aaatacgcat ctacaccttc
     9001 tttaatttga acaaaagccc ctttttctat tattctaaga actttaacat tgacaacatc
     9061 atcaatttgt acttcttttg ctattaaatc ccaagggtca ttatttatgt cttttaatgc
     9121 caaggcaatt tttttagtct ctttatttac atccaacaca taaacttcta cttcttgtcc
     9181 ctctgaaaca acatcttcaa cactttcaac tcttgtccaa gctaaattat ttatatgtgc
     9241 taatccttca atcccaccaa tatccacaaa tgcaccataa ggcattatct ttgtcacctt
     9301 accatctctt ttttgtccaa cttcaagaga attaaataag tcttctttag ctgctttgat
     9361 acgagctttt tcttcttctc tttcttgctt aattcttgct ctttcagctt ctttttctgc
     9421 cttaatttga gctcttcttt cttctctctc tttatttata cgctcttgta atacatctct
     9481 atgagatgct acaagtctat tttttctaac atccaattct tttatataaa cttctaagtt
     9541 aagaccaaca aatttagatg tatctgtaat aaattttgta tcaagttgag aaattggtat
     9601 aaaaaatgta tatgtattgt aattagcaaa tacaccttta tctatattct tttcaacatt
     9661 tacagttatt attctatgtt catcataagc cttttgtaac tcggcaaaat catttctttt
     9721 gtcaagttgt aatttagata aagttattgt accatctttt tgagatactt ttgttattac
     9781 agcagttatt tcatcaccta tgtggtatat atcttctata tattgatttt tttctatgtt
     9841 caattcactt ataggtatta ttccatcaaa cccatagtct aatcctaatt gtacttcatc
     9901 atttctcact gcagttattt ttccagttgt caattctcca actttcactt tgctgaatac
     9961 ttgttcttgc tggtcaagta attcttgcat tgttaaatca ttttccatta taaacatcct
    10021 ttcctcttat tgattatcat atctgtaaat attataccat taatattgca aatacttaca
    10081 tttttcttac tataattttt aaaataaatt caacttcata tatttataca ttatagaaaa
    10141 tatcttatta aattaaaata caaattctaa tttttatcta ataaaatatt ttcataaaaa
    10201 atttatattg tatatcttat tcttttattg ctttaggttt tgatagaaaa catttataaa
    10261 atataaagaa agaaattgct gttatagtag cggctgttat atctgcaact ggctctgcta
    10321 aaattactgc aaaaagtttt tcattaaaga atgttggaag tataaatatt aaaggtatta
    10381 aaagtacaat ctttctaagt agagctaaaa ccaaagatat tgttgctttt ccaagtgcta
    10441 aaaatgtctg ttgacaagct atttgaatcc caaacataaa agcaccagca aaatatattt
    10501 ttatactcca actagtcata ctaagtaact gcggattctt attaaatata ctaacaaaaa
    10561 atgcaggaaa tatcattaat aaaagccata aaatagtaga atatgtaaaa catgacaata
    10621 aacacaactt aaatgtcttt tttactctat caagttgacc tgaaccataa ttataactta
    10681 ttattggttg tgctccttgt gtaaccccca tattaggaag cataagaatt tgcattatac
    10741 tgctcattat ggccattgaa cctactgcta aatctcctcc atacatttgt aatttactat
    10801 ttaaggctat aagtactaaa ctttctgtag attgcattat aaatggagag attcccaagc
    10861 caagcactgg taagatgata cttgcttttg gtatcatata cttctttttt attttaagta
    10921 tacttttctt tccaaagaga aacataagta cccaaatagc agaaacgcct tgtgcaatta
    10981 tagtagctaa tgcagctcct tttacaccca tattaaagcc aaatataaaa atcgggtcaa
    11041 gtattatgtt tattaaagca cctatagcaa ctgtcatcat tccagttgta gcaaaccctt
    11101 gggtatttat aaaagtattc atacccattg aaatctgaac aaacactgta cctattaaat
    11161 aaatatttaa atagtccatt gaatatccaa ttgtagattc acttgctcca aacatccaaa
    11221 gtaaaggctc ttttccaatc ttaaaaccta tagtcaaaag tattgctaaa atcaccaata
    11281 cagaaaaact gttactcatt attttctctg cctcatcatt gttttgctca cccattttta
    11341 tagcagctaa tggacctcct cccataccga ttaaataact acatgcagtt attataatta
    11401 ttattggaaa tgccactcca acaccagcca tagcaacttc tccattttcc attctaccaa
    11461 taaaaatcct atcgactatg ttgtataaaa catttacaat ttgtgcgata atagcaggtg
    11521 tagcaagttt aaataataat ttacccacac tttcactacc taagttagtg tttgaatttt
    11581 ccattttaaa acctcctcaa tccaataatt aaaatgtatc actaaaattt tattctatat
    11641 aaaaatttca ttttattagc agacaaattt agagtacttt ccctacaagt aatactatac
    11701 tatatttctt ttgccatagt aaacatttta gaactaactg aagttaaata ctttatctca
    11761 ttttttattt attattaaaa atttttcaca tatactcata aaaaaactat ccaagtatat
    11821 tacttagata gttttatatt atatgtaaag aaagagctga aactataacc cctgataaca
    11881 aattgccaag aaatactccc ataaaagcat cttttattct catttttaat atggaagcta
    11941 tagcagatgc agtccatgtt cctgtagttg gcagtggtat acctacaaat agtattatac
    12001 caattatcga aacacttttt aatttcttca ttcttgagtt tatttttcta tcaattacat
    12061 cagcaatccc tttaaatagt ttcttccctc ttaaaaattg taatacatgt ctgaatgtca
    12121 atatcaaggg aatcgatacc aatgtagaac ctattacact aaatacataa acccatatag
    12181 gatttaagcc cattgcaata cctataggaa tggctcccct caattctgtt acaggaacca
    12241 tagataaaat taatatgtgt aaatatccca aactattccc tcctattcta agtgacatta
    12301 agttatgaat ttatttgctc tataacctca ccatcctttt tactaactaa aaatgcattt
    12361 ttagtcaatg aaagcattgg taaatcactc ttagagtctg aatatgcata tgaattttca
    12421 taatcaatct ctatcccaag tttacctaat acagccttta ttctttttac tttttcaatt
    12481 cccttacagt tttctccaat gaattgattt ttatatcttg aattttcata aaaaaactcc
    12541 gtgcctataa cctcgtctgc aagaccataa tacttaaaat atttcatata tgcatatgga
    12601 gatgcagtta tcataattat aacattatct tcttctttat ttttgtaaat aacctttttc
    12661 atagattcgt taattttttt cattatgcaa tcatcaaaaa acttttttaa atcttcttca
    12721 ctaaaatcta ctattggata aaagattgcc tcttttgcct tcttcaaggg tatcattcta
    12781 attaaataca taaatagaat aaaaataatg tatggcaact ttataatagt tttaaaggtt
    12841 ttatttttta aagagtaaat aaaaaaatct atcatgctat cagtatttat aacagtataa
    12901 tctacatcaa gtaataatac actttttttc ataagtattt ccccatccta tcactaaaaa
    12961 tatcactaaa aaatcaatat tattaaacat gtaattatcc ataagattac gtttattttt
    13021 aaagcattat cttttaggac aatatcagta ggattatttt catttgtagt gttaattata
    13081 taattatatc taagtacacc ataaactaca aatattgttg taaatatcat atgtggcttt
    13141 tctggattac ttgtagaata caatgcataa catacaattg tagaacttaa tataactatc
    13201 atcatttgat ttagattttc tatgtcataa tcttccaata tttttctatg ttcaactgca
    13261 tcatctctta gagtttctat ttctttttta cgttttccaa gagctaagta cagagataag
    13321 aaaaatgtac ataaaattat ccaatttgat aaactaacac ttatggctac acatcctgca
    13381 taaaccctaa gtataaaacc aagagatatt gaaaacacat ctaatagtat tatattcttt
    13441 agtttaagag aatataatat attgtttatc aaatagagac tgagaattat agaaagatta
    13501 aaactaacaa ctgttgataa acagactgaa attaacgcta atactacaaa tagtatatta
    13561 gcactttttt tgcttattct tccacttgcc actggtctat tacatttttt aggatgtttt
    13621 ttatctttat ccatgtctat agcatcattc agtgcatata ctgatgatga cattaggcaa
    13681 aaacacacaa acgcaataaa cgtatttaaa aataaattta tatttagtat gttgtttgaa
    13741 aaaattatag ctgaaaatac aaagaaattc tttatatatt gctttattct cattaacttt
    13801 atataatctg aaattttagt aagaagcctt atacttgtat tggtctgttt cataaactta
    13861 ctaccctttc aatattattt tttaaacagg atatgatttt atccctatgt ttaaaatttc
    13921 tataaatcca ttgacattac tgattactat agtaaccaat aacagcgata gaaatatatt
    13981 atacatattc ttttcatttt ttctaagggc tttcgtaaag taagccaata aaattataac
    14041 tgcaaaacta aaatttatag ccataatact tgaattgact agattatatc ctactatacc
    14101 atgtaaagtt agattgaata taattattcc catacatagc ttaggaactc ttttttctga
    14161 attttcaata actgataaaa cacaaaaaca taaagctaat aatgtaagaa ttactggtat
    14221 tgtagcaact tctccatttt gcaaaaataa ccctttttta atacttgctc caggaaaata
    14281 aaaaaccgaa tacaataatc tctctataat cattgcaatc ttttccaaaa ttggttctcc
    14341 acttataaat ttcgtatcat actgtattac atttccaagg ttcataaaac tctcataaga
    14401 cttcgttgaa actacacaaa tatatgccat taggataaat gtaattaaat gttttaacat
    14461 ttttcctatt tttaatttaa actcaaatat catattaaaa aaatacaaat atatattact
    14521 aatagttatt ccaaatagag gaatagctgc taattctacc aaataagaat tttttccttt
    14581 catcttattt gcaataacta tgaaaaatat taatgaaaat tggctatata taaatctctc
    14641 tacatttaaa gtcataaata actgtggaaa agaaaatatc attatcatag taagtaaaat
    14701 taaagttaat ctctgcattt ttatcttttt aatatgatta aagactaaag ttattgacat
    14761 agcactagtt attatttgta gacaaattag tccaaaccca taggcactat taaaatctgt
    14821 tgtcccagga tttgctattg aagtacaaaa taggtatatt ggatatgcaa aaattgatat
    14881 aattgctgaa aacaatatat gcttactatt gtcttgactt atagcaaata catttaaatt
    14941 gaataaaact cctgtatctg tatctaacaa tacatcataa gtattgttta aatcataaaa
    15001 tatagtagtc ttagaaaaaa atatcttagt tattattaag taagttatta caactaatat
    15061 aaacaaaaac atctcgtttt tatattttaa tattttactc atcaatatat caccatccat
    15121 tcattatata ggatttgctg acacctacaa tttattcctt atatttaata gccatcttca
    15181 taatgttctt acttgtttta ttgtaattga ataactcttt atttacaacc acaatattta
    15241 ctttattgat gttataatca gtcaagtatt gtttatataa catagattag tgttgtataa
    15301 aatgagaatt tgtaagcaat attaatgtaa aatatttgaa aaactttata taaaaatata
    15361 caatgttact tatggaatta aactataaca aattaaagca taatttaagg agacaaaatt
    15421 ataatctgtc tccttaaata ttaaaactat attttaaaac attactaaat actatacaaa
    15481 tttcaaatat agtataaaat taattagact ttaacactat tagctccatc ttcttcccat
    15541 tttttaattt cttttaatct atctattata ggtaaatgct gagtacattc aacttcacat
    15601 tttccacaag atatacattc tttagctttt gccttactat tcattgtgta attccaatgt
    15661 cctaagccat aaactaactt tgtcatttct tcatcactct ttttaaacat ctgcttttca
    15721 ttataaaaaa gcatataggc tggggtatta ataccttttg gacatacttt acaataacca
    15781 caacctgtac atatttcgtt catattctta tttaatctag tctcaatatc atgtatatcc
    15841 tcatctgaat aaattctatt ttcgtctgca attttacatg catcatctat atcttgtttt
    15901 ttattaaacc ctatcaaaga tatacttatt tgtgaaattc ctatgttgaa tcttaaagct
    15961 gattctatag tactttcgcc atcttttgat agaaaagaaa gttctttatt atactttgga
    16021 atagttccac cacttaatgg attcattgcc acaacaccat atccattatc aacagcatat
    16081 ttggcacctt ttagcctata tggaaagttt aatatattta ttcccatagt tacaccttca
    16141 aacttatttt cacttagaat atctataacc tcatctcctg gttgatgaga tgaaaatact
    16201 atatggtcta tcaatccttc ttctttacat ttcaataatc cttcatattg acctccaggt
    16261 ctcattgcta gttcgtaatg ttccatcttt ctaatattcc aaacatgata aaaatttatt
    16321 ttagatacgc ctaatcgttc tattgatttt tttacagcct ctatagcttt ttctgatgta
    16381 tcacatgctt ttggcttttc tttagttgat acataaaaat cggtcttacc ttcctttatc
    16441 atttgtctaa aggctagtcc aaatatatct tcacttctgt catcacaata ccctggtgct
    16501 gtatcaaagt aattaatacc tttttcatac gcatatttga ctaaatcagc attttcttga
    16561 ttactttttt ctaaatcaaa ccttaatcct ccatagccaa ctactgaaac cattttccct
    16621 gtctttccat attcaacgta tctcattgta aattcctcct tagatatatt aagacttaat
    16681 ttctttatag aaaatattat accataaata atttttataa attatttatg gtataatatt
    16741 ttttttataa attatatttt tttataactt gtattttata gctaagttaa aaactataaa
    16801 aaggttcaat atggactatt acttgagcat tttcattaat ttcttcttgt atttgtttct
    16861 caattttatg tgttaaatca tgagattgct ctacgcttat aaatgggtct accattacat
    16921 gcatgtcaat ataaatagaa cttttgctac ctctacttct gatattgtga acatccctta
    16981 cctcattaaa ttcaaacaca atttctttta tataatcctc atctacagcc actctatcga
    17041 ccaaaattcc aatagatggc ctaaatattc cataagccga ataaattatg aatgcagaaa
    17101 taataatagg aactatagat tctattatta ctggaaatcc taacttaaca ccaactaaag
    17161 taaccaatac acctagagac acaaatatat cacttcttgt atgcaatgaa tccgaaatca
    17221 aaacataact atttagttta gtcccaattc tatattcata catgcataca aaaatattta
    17281 taaaaagtgt aataattaaa gcaattaaac tttctatagt tatagcaggt acaactggat
    17341 tagctattct taacactgca ctaagaatta tcttaattgc tataacaaac aacataccac
    17401 ttataaataa actggttata atttcaaact ttttatgtcc ataaggatga tttttatctt
    17461 ttggtttaga tgcaaagaaa atacctataa gccccactat attagatgct ccatctgaca
    17521 aagaatgaaa tccgtctgct gtcatactat agctttttat ttgattacca ataatgattt
    17581 ttaataatgc tactgcaaaa ttagcaaata aaattatcca caatacttgc ttcactttct
    17641 tatagttatc cataacaaat tccccctccc ttcgattcaa taaagaaagc tatctatcaa
    17701 tatcaatcaa ccagacaata aaatcgtata taagcacatt tactatattt atattcaaaa
    17761 ttggttgtac ttgttcattc tatttgaaac ttaattataa ataaaaatcg taggtatggt
    17821 tttggtatac ataaaaataa gagactgtct caaaataaag ataatcttct tttttattgt
    17881 tgaaatgata aaatatattt gatttcatat aaaaaagagt gcctcatgaa ctaaaaagtt
    17941 cattttgaga cactctctta aattttttga tataaactat taatttttta tactaagcct
    18001 tctttaacta agaatgaatg agcaacttct tctggtgatt taccaagttc atccacttga
    18061 tagttaagtt ctctcatagt ttcttcattt atttttcctg aaagcatatt cataactttt
    18121 tctaactcag gatatttctt caaagtatct tctcttacta atggtgctgc ataatatggt
    18181 acaaagaaat ttttatcgtc ctctaatatc ttaagattaa actttttaat aagaccatca
    18241 gttaaaaatg catctataac ctgagattca ttattatcta aagctgtata tcttaaactt
    18301 ccattcattg cttttacatc tttgaatttt aaaccataat ctctagaaag ccctacaaga
    18361 ccatcttctc tattttcaaa ctccaaagtt ggtgagaatg taaattcttt accatattta
    18421 gtcaaatcag aaatagtatt tattccatac ttttcagcag tttcaggcat catagctaat
    18481 gtgtaagtat tattaaatcc aagagggtca agtaaagtta actgattttc tttttccata
    18541 gtatctttta caacattata tgcttcatca gcatctttaa tcggagcatg tttcattaca
    18601 tttaccaaaa gtgtacctgt atagtcaaca tacatgtcta actcaccaga tttaattgca
    18661 ttaaacgcaa cagatgaacc acctagattt aaattcttct taactttaag attcgtattt
    18721 ttttctacta attcagcata aacattacca agtataagtt gttctgtata atctttagaa
    18781 ccaatagtta ttgtatcttt tttacttgaa aaactagaac taaaaacaac gaacaacatt
    18841 gaaacaacaa ccactacaga tattacttta agtgctatat aattcttctt aggtgctttt
    18901 ggatttaatc cttttggagt aactgcaact tcaattttac taaataaata atcaactaac
    18961 agtgcaatta tacatgatgg gattgcccct gctaaaatca tattattatt tacagtttga
    19021 acccctgaaa atactaaata tcctaaacca ccagcaccta taaatgctgc aagagtcatc
    19081 aaaccaacag ctgtaactgc agatattcta actccaccca ttattatagg aagtgctaaa
    19141 gggaattgta ttttaaataa tgtctgattt cttgtaagac caataccttt agctgattct
    19201 aatactactg ggtcaatact tgttatacct atatacgtat tttttacaat tggtagtaat
    19261 gaataaacca ctaccataaa tatggctggt ttacttccta taccaagtat aggtacaaga
    19321 agacccaaaa gtgccataga cggaactgct tggactaagt ttacaaatcc aagtacaaac
    19381 tttctaagag atgagattct tgaaataatt attccaagag gaacaccaac tattatagct
    19441 attaaaatag atgtaacagt tagactcata tgttgtatta aaagctctat aatcttatct
    19501 ttttgtaata gtataaagtt aaaaaaatta gccaagaaca ctcacctcca tttctaagaa
    19561 ttgttcactt aaaacagaaa gtaaactact tctagtaata agccctacca gtttattttc
    19621 atcagaaata accggaatat atccaaccga attacgattc attacattta gtatttctac
    19681 taaattatcc ccttcattta catgcaaagg ctcacttgac ataatatcag caagtaatat
    19741 tgacttatca tcaatatctt tcatatcttt tacagtaaca atacctttta atgtttttgc
    19801 tctatctata attagtaagc tatcaacctt actcgttctc ataatttcta ttccttgagt
    19861 tacagttcta gtagaattaa cagcaatagg attttttatc ataatatctc ttgcttttat
    19921 atattctgga ttatcccaaa ctctatcttc tccgataaaa tcttttacaa agtcatttgc
    19981 aggctttcta agtatatttt cgggagtatc atattgtgct attcttccat ccttcataat
    20041 acaaatttta tcagctatct ttaaagcctc atccatatca tgcgttacaa atattatagt
    20101 ttttttcaat tcatcttgaa gagaaaataa ttgttcttgt aaagatgttc tcgttattgg
    20161 gtctaaagca ctaaaaggct catccatcaa tattatttca gcatcagtag caatggctct
    20221 tgcaacaccg attctttgtt gctgtccacc actaagttct gaaggatact tattcaaaaa
    20281 ttcatcacta tcaagtccta ccatctcaag tagttgcaac gttctttttt caatttcttc
    20341 aacggatttt tcctttttta ggcgtggaat tagctctata ttttctttta ttgtaagatg
    20401 agggaatagg cctgtgttct gtataacata tcctattttt cttctaagct ctatttcatt
    20461 ttcctgagaa attggtttgc cattaatata tatctcacca gatgtaggtt ttattaattt
    20521 gtttataagc ttcaaagtag tagtctttcc acaaccactt gaaccaatca aaactaccaa
    20581 tgtacctgtt tctacaacta gagaaatatc atctaatata acattatttc ctattttttt
    20641 tgttacattt cttatctcta tcataattta tcatcccctt tttcgaacaa gcaaccttct
    20701 ccaatattat aagttgtttt tataattata tcatactatt ttatttatgt aaacaaagta
    20761 cgatttttag gtcactattt gtctgaatat tgctacaaat ataaagtatt tatatatagt
    20821 atataatata caatgtcaaa aaacaaaaaa tataaaaata ttcataaaat aacactagcc
    20881 tcaattatat agtattaaag ctagtgttat ttcatttaaa caaaactcac atttggatac
    20941 tatccatgta ttcaaataat gtaatatctt tataagtttc ttttttatca cttaggtttg
    21001 atattgtaac tcccaatagc ctaatttgtt ttccattaac ctcaacattt ctcaacaaat
    21061 tatctacacc acttcttata tcattatatt cgtcaatata gtgttctaaa ctaaggcttc
    21121 tagttatctt tgtaaagtcc tcatatttaa tttttaatgt taatgttttt ccaaacttct
    21181 cagagttttt taatctatga catacttctt cacaaagttc atcaagaatg ttgcgtgttt
    21241 cttcttcatc aatatccaaa ttatgactta atgtagtctc agccccaaca gattttctaa
    21301 ccctatttgg ttccactggt ctatcgtcta ttcctctagc aaatttatat aattcataac
    21361 ctcttttttt gaatatattt tcaagttcaa ataaatttaa acaccttaaa tcatatccag
    21421 tttttatacc taaattttta agagtatttg aagtaacttt tccaacacca aaaaatttat
    21481 tcacaggtaa tttatctaaa aagtcctggg cattttcttc ggttattaca gttaacccat
    21541 ttggctttct taagtctgat gcaatcttag caagaaactt attgtatgac acaccagctg
    21601 aagaagtaag accaacttct ctaaatatat ctttttttat ttgctttgct atctcaattg
    21661 atgaatcaat atttttttta ttttttgtta catctaagta agcttcatct aaagataatg
    21721 gttcaactaa atctgtgtat ctataaaata tatctcttat ttgctctgac acactcttat
    21781 aaacatcaaa tctaggtctt acaaaaactg catatggaca cctattgtaa gctacagctg
    21841 atggcatagc tgaatggata ccaaattttc tagcttcata agaacaggtt gcaaccacac
    21901 ctcggctttg tggatttcca ccgacaataa caggtcttcc ttttaatttt ttattatctc
    21961 tttgttctat agatgcataa aaagcgtcca tatctatatg aattattttt ctataatgtt
    22021 tcattattat caccttttaa agtataaata gattaatttt atttatatta ctttttaatt
    22081 ataccaaact tatgtttctt tttggtattt tttcattatt ttctttatat attcatattt
    22141 tccataaata aaaccatcta taatccatta acttcaatat aatttataaa aaatccttaa
    22201 aaacacttgc aaaaactata taaaagcttt acaataggta ttccaaggaa ctaaatttaa
    22261 aattccttat ggataacagt atatatattt aatttatggg atgttttaaa aacaaaagac
    22321 tgtcttatac aagacagcct actgatttta ttataattgt aatggactgc tcaaactatt
    22381 actttaaaac agcccatatt tttttatata attttaacta tagtaaagat tttgtgaagg
    22441 atattctggg tcacaagtta catctatacc aagttttcta aatgtttgtt catcatctct
    22501 atttaatata gtagtagaat gagcttgaca cccttttaat aatgctaact tttccatagc
    22561 aaattgtgca gttggattag ttacagcaca aatactaagt gcagttaaca cctcttggca
    22621 actaagtgaa acatttctac tagctaaagt ctttgttttt aagtttatga taggttctaa
    22681 tattacaggt gatattaagt gcatatcatc agatatattg gctaaatgtt taatggcatt
    22741 taatactact gctgcagttc cattcattaa atcagaaccc ttaccagtta atattgtacc
    22801 atcttctaat tctaatgcaa ctacaggaca agtatcattc ttgtcggcac tttctttcaa
    22861 tttattactg tagtttctag caggtaatac aacatttcta tcttctggct ttaagttaag
    22921 ctcttccata atcattttca ttctatcata agcaccttta tcaacttgtc cctttttata
    22981 ctcacaggca gttttaaagt atcttcttat tatttcttct atagaagctt ttcttattac
    23041 ttcatcatct attattccaa agccaactct attaacaccc atatcagttg gagattggaa
    23101 aattgattct tttccagtta tcttttctat tattttttta agcactggaa ataattccaa
    23161 atctctattg tagtttactg acatttcacc atacgtttca aggtggaatg aatctatcat
    23221 attcacatct tttaaatcaa ctgtagctgc ttcataagct atattcaaag gatgttttac
    23281 aggtacattc caaactggga atgtctcaaa cttagaatat ccaactacat tacctctttt
    23341 attttcatga tacaactgac ttagacaagt accaagtttt ccactattag gtcctggtgc
    23401 tgtgactact actattggct ttgtagtttc tatatattca tttgctccat atccctcatc
    23461 actaactata gtctcaacat ctgacggata tccctttgtt ggataatgct tataaacttt
    23521 aattcctcta cgttctaatt tatttataaa tacagttgta gcaggttgtc cttcaaatct
    23581 agtaattact acactattta catctagttc ataacctctt aaatcatcta ttaatcttaa
    23641 aacttccata tcataagtga taccaaagtc tcctcttatt ttgtttcttt ctatatctcc
    23701 tgcatagaca caaattacaa cttcaacttt ttctttcata tgttgtaata cttttatctt
    23761 tgcattttca tcaaatcctg gaagtactct ttttgcatga aggtctccca tcaattttcc
    23821 tccaaactct aaataaagtt tgtcaaaatt atttactctt tctaagatat atttagactg
    23881 ctcttctaaa tattttttgt ggtcaaatcc tactttcatc ttaacacctc actaaattaa
    23941 atttcttgtt gataacagta tatttatata tattttttta gtaaaacatc ttataaaaaa
    24001 atgttaatac tttttcactc aaagtaatat tatacaatta agattaattt ttttcaagat
    24061 agttttatgg t
//
LOCUS       DAMJYR010000052        22745 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.052, whole genome shotgun sequence.
ACCESSION   DAMJYR010000052 DAMJYR010000000
VERSION     DAMJYR010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 22745)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22745)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22745
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.052"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            223..1659
                     /locus_tag="PC471_002015"
     CDS             223..1659
                     /locus_tag="PC471_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HDF2789144.1"
                     /translation="MICTIKPLKISIKKSTEPLIEFISDTTYKNKSSSYIKEVYFTRY
                     IGVDDSIDKYIEKIKNIDTYFFNNPTIPYIRLNNLKINFDKEQTDKMLKIFNIYNSSK
                     FNSWDLYNIDFPCKIENDTLNWTKKIAFKNTLDLFSTSMPNCTSSIIKNFAVKLLCWI
                     DYFLPKLFYNKVKTTISPKIVYFGNIKRQELFFLYFLSQLGCDILYINPNKDILDLYP
                     ECSKFSTLFELSRKTPNILEIPFERHITNINYSENTNKTSPTTNKPNSKNTLNNTNLT
                     SKKIVQDEEVELSYEDLAKLSASVVMIVVCNNENKPFKSGSGVVINNEGYILTNLHVV
                     NDGYSFLVRFENDDKVYTSYQIIKYHSDYDLAVIKVDRKCKPIPVKVSKKPVRGQKIV
                     AIGSPLGLFNTVSDGIISAFRDFETVQMIQFTAPISSGSSGGALLDMFGNLLGLISAG
                     YDDGQNLNLAVESSLVKIFANNFIEIVN"
     gene            complement(1751..3625)
                     /gene="asnB"
                     /locus_tag="PC471_002016"
     CDS             complement(1751..3625)
                     /gene="asnB"
                     /locus_tag="PC471_002016"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HDF2789145.1"
                     /translation="MDGNISIYYKGDISKKEDIKFKIKESVNNTRLSSRIEYKDEEKT
                     FDFERFNVIDAIYGNKPFEKDGKVIVFNGNIYNYHEIKEELIKKGHNFTTNDDIEVLL
                     ASYIEFGKNCVHKIKGMFNFIIYDRENESIFGARDLFGIKPLYYINKENAIIFSSEYK
                     FLLEYMKNLNINERSLQSYFSFQYVLPEDTMIQGIRLIPAGHYFRVENGILSLKRYNK
                     LEFRSSTKFFYTKNHLGNRDVNEDDIRNIVVDSIVTHMEEEKEIGTFLSGGIDSSIIT
                     TVASQINPNIKSFSAGFSVKGYSELEVAKKTADKLGIENIQINITQDEYIKSLPNVIY
                     SLDDPIADPSEVGLYFLIKEAGKHVKVALSGEGADELFGGYNIYKEYSTMKSVVNSPT
                     YIKSILGRVSELMPNIKGRNYLYRATTPLEKRYIGNAKVFENSEVKRFFFKYKEKNIY
                     EYLLSNLYRDAQKNNYDYISKMQHIDVNTWLQGDILQKISKLSTAEQVELRVPFLYKD
                     VFDVAKNLRMEQKINKNNTKVLLREAFREIVPEHVVQRKKLGFPTPIRVWLKDSLGDI
                     VKETIYNSNVDEFIDKKYAIKLLDTHLKGHRDNSRKIWTIFTFCLWHQLFIEHKNVEY
                     "
     gene            3828..4502
                     /locus_tag="PC471_002017"
     CDS             3828..4502
                     /locus_tag="PC471_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000094049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HDF2789146.1"
                     /translation="MSLFIISQCITSFAFTPENNKFKVKPLPYAYDALEPYIDKETMK
                     LHHDKHYQAYVDKLNAALEKYPELYNYSLCELLQNLDSLPKDIATTVRNNAGGAYNHK
                     FFFDIMTPEKTIPSESLKEAIDRDFGSFEKFKQEFQKSALDVFGSGWAWLVATKDGKL
                     SIMTTPNQDSPVSKNLTPIIGLDVWEHAYYLKYQNRRNEYIDNWFNVVNWNGALENYK
                     NLKSQD"
     gene            4615..5151
                     /locus_tag="PC471_002018"
     CDS             4615..5151
                     /locus_tag="PC471_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HDF2789147.1"
                     /translation="MIKETHERGGYIFALLILPFINNLYLVKYDIAYKIVLIIIYIYF
                     AYLGSLFPDIDMRGSYISKKFMLIYKLFGSRFRHRGFTHSLIALLLIYSFFKSLTIFT
                     NNNIVFSCLSSGFIIGYLSHLCLDLITKEGIELFYPITINISLLPIKTNSKTEKFISK
                     LLNFIVIFLIGYQFYILL"
     gene            complement(5317..5937)
                     /locus_tag="PC471_002019"
     CDS             complement(5317..5937)
                     /locus_tag="PC471_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HDF2789148.1"
                     /translation="MQYIQELLDLTQNYWVLATIIGLLSAFIESFIPALPLVAIVTAN
                     AAIQGLFIGCLLSWIGSGLGTTSLFLLISRFNDSKLFNKLRNSKTEKAISWMDKQGFK
                     LLFIAYACPFMPGCLVTIASAFGKKDLKDFVPAMLAGKFVMFIVISYVASDIEGFITS
                     PLKIASFILLVFLSWKIGNRVNKNLENHNYDFHHKKHHNDKDDKMI"
     gene            complement(6251..8389)
                     /gene="vanT"
                     /locus_tag="PC471_002020"
     CDS             complement(6251..8389)
                     /gene="vanT"
                     /locus_tag="PC471_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021418922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine racemase VanT catalytic subunit"
                     /protein_id="HDF2789149.1"
                     /translation="MIRNKSYAGLDYFRIIAALLIIAIHTSPLLSYSKMADFVLTSII
                     ARIAVPFFFMLSGFFLISTYNYNCNKLKSFLKNVLFIYGIAIVIYIPLNIYTGYFSMN
                     NLVPNIIKDIVFDGTLYHLWYLPASIIGASIAYFLVKRLGLKYSLVITIVIYVIGMFG
                     DSYYGFLEKIPLLKNLYGYIFEVSDYTRNGIFFAPVFFILGGIISNKSNYMSTKNSLI
                     GFLISFLLMLCEGMFLHKLGVQRHDSMYIMLLPCMYFLFTALTFLKGSRARLLRTSSL
                     IIYIIHPMMIVVIRMFSKIFGIQTVLVDNSLIHYIVVSIASFIASIIIILILKPLKKD
                     LKGEHKVNTNRAWIEIDLNNLEHNVKMIQKAMPEDCELMAVVKANAYGHSAFEVATCA
                     NQIGVKAFAVATIDEGIDLRRYGILGEILILGYTNPIRAKELHKYNLTQSVIDLNHAI
                     SLNKQKYNIKSHIKIDTGMHRLGFDVNDVRSILKAFKLKYLDICGIFTHLCVSDSLKD
                     EDVDFTNKQIECFYKLIDFLLKKGIKIPKVHIQSSYGFFNYPNLKCNYVRIGIALYGI
                     LSSPNDTTKLQLDLRPVLSLKSQVILIRKIQKDDSFGYGRVFIASRDSVIAILSIGYA
                     DGIPRNLSCGKSYVIINGCRAAIVGRICMDQLAVDITDIPNVEVGNTAIIIGRDNLSE
                     LSASEVANNSCSISNELLSRVGRRLNVIKK"
     gene            complement(8407..9213)
                     /locus_tag="PC471_002021"
     CDS             complement(8407..9213)
                     /locus_tag="PC471_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HDF2789150.1"
                     /translation="MMNNILLDKNKEDKMTNTILLTKDKVYDGNLILVNAFLPVKTSE
                     DIDLIPVDTRFPSILMKREATNILQNILKSICGINEIVPVSGYRTAEEQQDIYSSSLR
                     DNGKDFTKKFVALPNHSEHQTGLAIDLGLKKEVIDFICPEFPYNGICNEFRKAALHYG
                     FIERYQCKKEEITGISQEPWHFRYVGYPHSEIINKMNLALEEYIEYIKRFSYTNCLKI
                     EDKEKIIEIFYVPIHTKEEVRISIPEQCVYQISGNNKDGFIVTFWRNRKD"
     gene            complement(9210..10310)
                     /gene="vanG"
                     /locus_tag="PC471_002022"
     CDS             complement(9210..10310)
                     /gene="vanG"
                     /locus_tag="PC471_002022"
                     /EC_number="6.3.2.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021418924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-serine ligase VanG"
                     /protein_id="HDF2789151.1"
                     /translation="MNNKKIAVIFGGNSTEYDVSLQSAFAVLENLNTEKYDIIPIGIT
                     REGNWYHYIGDYNNIQNNTWFERDTDIIPVIVSQNRVKNGIIEFLIPDAVSQNHIPNS
                     AIESCENDIKYTKLDFVFPVLHGKNGEDGTVQGLFELAGIQVIGCGTLSSALCMDKDK
                     SHKLVHSEGIKVPKSILLKYSDKFNVAELSKDLTYPLFIKPVRAGSSFGISKIYKKEE
                     LQVAVDLAFEHDNEVIIEENIDGFEVGCAVLGNDDLLVGRVDEIELSKGFFDYTEKYT
                     LKSSKIHMPARIDLDCEKRIQETAKIIYRTLGCSGFARVDMFLTPSGEIIFNEVNTIP
                     GFTSHSRYPNMMKGIGLSFEEILDKIIEVYIK"
     gene            complement(10571..11653)
                     /locus_tag="PC471_002023"
     CDS             complement(10571..11653)
                     /locus_tag="PC471_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF2789152.1"
                     /translation="MRTALMAFIAIIFIWILYSFILYGKFSIWIVSFFENAFRMEYKA
                     ALNLYQQIFRNSMELFMILAMVIVFFIIFRVYLNWFTKYFSEINQGIDSLIKEDVGEV
                     ALSPELLAIEKKINSIKHILEQRKFETQMAEQRKNELIVYLAHDLKTPLTSVIGYLTL
                     LRDESQISEELRKKYLSISLDKAERLEDLINEFFEITRFNLSNISLEYSTVSLTRMLE
                     QLTYEFKPTLMSKNLKYTLNVTPNMMIRCDVNKMQRVFDNLLRNAVYYSFEDTTIEIT
                     AVQEDDYIKLKFINHSNTIPEEKLERIFEQFYRLDTSRNSNSGRAGLGLAIAKEIVEL
                     HNGTITAHSENNIIIFKVIIPIVGNL"
     gene            complement(11703..12404)
                     /locus_tag="PC471_002024"
     CDS             complement(11703..12404)
                     /locus_tag="PC471_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021418928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanG-Cd-type vancomycin resistance DNA-binding
                     response regulator VanR"
                     /protein_id="HDF2789153.1"
                     /translation="MNENILIVDDEKEIADLIEVYLKNEGFTVYKFYTGKEALECIES
                     KNLDMAILDIMLPDIDGFHICQKIREKYYYPIIMLTAKIEDIDKIMGLTIGADDYITK
                     PFNPLEVVARVKTQLRRYMRYNNSYEQQSIIVNEYDIKGLIINKETHKCSLFGKEVAL
                     TPIEFSILWYLCEHQGKVVPSEELFEAVWGEKYLDNNNTVMAHIGRLREKIHEPSKNP
                     KFIKTVWGVGYTIEK"
     gene            complement(12838..15369)
                     /locus_tag="PC471_002025"
     CDS             complement(12838..15369)
                     /locus_tag="PC471_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HDF2789154.1"
                     /translation="MSLKSLKIKENLYWVGSLDPDLRVFDIIMYTPYGTTYNSYVLKG
                     TEKTVLFETVKDKHFDNYIERLNDLNIDFEKIDYIVVSHTEPDHAGSVEKLLDLAKNA
                     KVVASETAIKYLKEIVNKDFEYVAVTDGDTLSIGDKTLEFFSVPMLHWPDTIYTYIKE
                     DKTLVTCDSFGSHYSNDKIVNTLDENEEKDYLDALRYYYDCIMGPFKPSMVTAIEKIK
                     DLDIDTVCPGHGPVLTENPRKIIDLYYNWSVNEQIKLEKEVTICYVSAHGYTKIMAEA
                     IKAYIEKNSNYKVNLFDVIEHKQEDILAKIAVSQGVLFGTPTILGDALKPIWDILISL
                     NPVLHGGKVASVFGSYGWSGEGIENAMERISQLRMTAVKPFAVNFKPSNEEIDKLYSY
                     TGKFLDKLNSTFGSKKKTKKFKCVICNEVFEGDSAPSVCPVCGAKEDQFIEVEEDEVT
                     FRKDTDEYFVIVGNGAAGFYAADAIRKRNKTCKITMISNEDELTYYRPALSDGINEEL
                     GSDFYMEDKDWYDKNNIVVILGTNVDKLDEVNKTIIVNDGAIKFDKLVIATGSRNFIP
                     PIKGHDLENVFTLRNIKDLYSVKEALEKSKKVVVIGGGLLGLEAAWEFRLKGLEVVVV
                     EAMDSILSKQLDKEGSKILEQCVRDTGIDVRLGVAVDGIEGDGKAQKVVFKDGDSVDC
                     DMVVFSIGVRANTQMVQDTSVKIDRGIVVDKTLQTNVKDIYACGDVAQVGNISLAIWP
                     SSVEMGKIAGANASGDNLTFESEVYPVSLDAMNVKVFSIGNIQNFDKEISSKDEGQRI
                     YKKLFMKDGSLVGAILINDLSCTVKLIRLISEKGDFEDIMKADIL"
     gene            complement(15477..16094)
                     /locus_tag="PC471_002026"
     CDS             complement(15477..16094)
                     /locus_tag="PC471_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HDF2789155.1"
                     /translation="MCKKTYLLVVSMLIAGLLTACSGSPTKDSGQAQDNTKKEANASN
                     NALKDEKNNENLMEQDFKVPYTDAINIFKDKYKDADIVDLSLERDLNKFVYTVEGVDD
                     NNEYKMKIDANTKDVLEDKTEKLDSEDLNGVARKEKLDLNDIMTPQQAMEIALKEQNG
                     IVKEWSLDKDLDVTFYKIRIDKDKNEYDIKVDSKKGTVLKVEKED"
     gene            complement(16456..17340)
                     /locus_tag="PC471_002027"
     CDS             complement(16456..17340)
                     /locus_tag="PC471_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HDF2789156.1"
                     /translation="MDTTKIDDISKLDLNNLDINKMDMGGIMESLLEWATTSGVKLII
                     GILILSIGFKIIKKFVNHVMLLLSKRDIDLTLRKFLKSLLLSVLKVAVIIIVLEYWGM
                     SLSSFAAVIASAGVAIGLALQGSLSNFAGGFIILLIRPFKVGDYVEAAGHGGTVEQIG
                     LFYTQLVTPDNKQILIPNGSVSNDSLINYSAKNTRRVDLTFSVGYEDDILHVRRVLKD
                     IVNNHKLIINEPEPFIGVVEHGDNAIKFATRVWCKTEDYWTIYLDLLEEVKVRFDEEG
                     ITIPYPKMDLTVKELNKI"
     gene            complement(17531..18781)
                     /locus_tag="PC471_002028"
     CDS             complement(17531..18781)
                     /locus_tag="PC471_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDF2789157.1"
                     /translation="MKSYTKAKWSVWGIITFSFVLVLFLRMSTAVVSDNLANELGFNS
                     IQISNIASFCLYAYAFMQIPAGILIDKYGARKISSLGIIMASLGSILFGLIQSIELAY
                     ISRVIVGAGTSVILLCILKIQGRWFNKSEFASATAKFSFVGNLGGVLATFPLVFLSEL
                     VGWRNSFLLIGIIGVVIGCFMYIIVRDTPKEYGFNVDTEPYEKSEKVNIVDGIKSVIK
                     NKSTWYNSMIMFSFVGLTSAFISLWGVRYIMDVYGVSKSFSAFIVSFFTYGFIFGSII
                     MDFVFAKIRSSKFNIIKFGAMIDLFIWIVIVVVYQVKPPIIVLPISFFIMGCIVMSHL
                     QVFNDAKYKNKEIYSGLATSVINTFEFIGSGIINLIIAISLQVNSYNIVDGYKKGFVV
                     FIVLSIITIVSSHIGVKNDDFKAI"
     gene            complement(19003..19767)
                     /locus_tag="PC471_002029"
     CDS             complement(19003..19767)
                     /locus_tag="PC471_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2789158.1"
                     /translation="MLGKLLKYELKASGRIFIPLYIAILIVAVFNGIFMNTNILQVQG
                     IGILVLTSLFMALGVLTIVVTIQRFRKNLLGDEGYLMFTLPVSTSSLILSKCITALIY
                     AVLSFIVAVFTFGVLMLFGTSGILLPEILDLFNTSFKWISENFLDILLLVVVMFISYS
                     SFILLLYTSISMGQLPKFNKHRNIVAFASFIAINIVISIVGDAVGSILPNEDTNMVYH
                     LYQSPSFMLAILGSLVVAIALFFATKFILDKKLNLE"
     gene            complement(19761..20486)
                     /locus_tag="PC471_002030"
     CDS             complement(19761..20486)
                     /locus_tag="PC471_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2789159.1"
                     /translation="MDKNISNVSQDGIVEFKGVNKSYGTKNVLKNIDLNIPKGKIVGL
                     LGPNGSGKSTMIKLMNGLLQPDNGEIMINGMKPSIDTKKIVSYLPERTYLNDWMKVSD
                     LLKFFYDFYSDFDVRRANEMIKSLDIDVNEKLKTMSKGTKEKVQLILVMSRNASIYIL
                     DEPIGGVDPAARSYILKTILKNYSEDSTLLIATHLISEIENICDEIIFISKGEIVLQG
                     DVEAIREEKGKSIDALFREEFKC"
     gene            complement(20488..20856)
                     /locus_tag="PC471_002031"
     CDS             complement(20488..20856)
                     /locus_tag="PC471_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDF2789160.1"
                     /translation="MEWELDNNKPIYIQLVEHLKLKIISGEIKIGSKLETVRALAEDA
                     EVNPNTMQKALTELERQGLVYSQRTKGRFVTDDKEKIKAMKEEIANVEINTLKVTLEK
                     LGYDRDEMLKLITENLKGEL"
     gene            complement(21032..22576)
                     /locus_tag="PC471_002032"
     CDS             complement(21032..22576)
                     /locus_tag="PC471_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HDF2789161.1"
                     /translation="MRNLQKKLMFFLFICIILQITYTTPVYSLCKLYKNNSINNFESI
                     VSNNQLISKHAIQIILIMAIICVILIFYIIYDKLNFKIKLQRIAYTDNLTGANTIDKF
                     VIDANKILCKNTQVKYALLYIDIDKFKYINDLFGYEVGNEILRNLTKIIKSNIFEEEM
                     FARISADNFIIIMKYIEEEDITKRLKTIFEELDLFNNNQEEKYKLVLSCGIYFILPED
                     RDINSIIDRANIPHKMAKGGHKSSYAFYDNKIHDQEIKEKEMENTMFSSLENKEFIIY
                     LQPKIELNTGEIQGSEALVRWKRPDKGLIPPNEFIPFFERNGFVINLDLYVLEEVCIY
                     LRKWIDAGINPVTVSVNVSRIHLYCNNFIETYKNIIDKYNIPAKYIELELTESIIFDN
                     FDILIDIMNNLKKIGFLISMDDFGSGYSSLNMLKEIPMDILKLDQKFIMETYNSKRSK
                     IIVTKVIEMAKELGMKVISEGVETEEQFKLLKEVKCDMAQGYLFGKPMPIEEFEHLIV
                     SNLVRG"
BASE COUNT         7839 a         3833 c         2376 g         8697 t
ORIGIN      
        1 tagcggtatt agattacaag cattattcta acttttttta tattaagacc ttacctttca
       61 tccctacacc taaaggagag ggcttttcgg caattttatt gtaattatat atcatttata
      121 taaacaagta catattttat tttgaaacta caaaattatt gtactatatt gttttaagtt
      181 gttttataat agattatatc atcttattat aggagaaaaa atatgatttg tactattaaa
      241 ccattaaaga tttcaataaa aaagtctact gaacctctaa ttgaatttat atctgatact
      301 acctataaaa ataagagttc ttcatatatt aaagaagtct actttacaag atatataggt
      361 gttgatgatt caattgataa atacatagaa aaaattaaaa atatagatac ttattttttt
      421 aataatccta caattcccta tattagatta aacaatctaa aaataaattt tgacaaagaa
      481 caaacagata agatgcttaa aatttttaat atttataata gttctaagtt taacagttgg
      541 gatttatata atatcgactt tccatgtaaa atagaaaatg acaccttaaa ttggactaaa
      601 aagattgcat ttaaaaatac tttagattta ttttccacaa gtatgccaaa ttgtacatcc
      661 tctatcatca aaaactttgc agttaaactt ttgtgttgga tagactattt tctacctaaa
      721 ttattttata acaaggtgaa gactactatt tcaccaaaga tagtatactt tggaaatatt
      781 aaaaggcaag aacttttttt cttatatttt ttatcacaac ttgggtgtga tattttatat
      841 ataaatccaa ataaagatat tcttgattta tatcctgaat gttcaaaatt ttctactcta
      901 tttgaacttt ctaggaagac tccaaatata ttagaaatac cttttgaaag gcatattaca
      961 aatataaatt acagtgaaaa taccaataag acttctccta ctacgaataa acctaactct
     1021 aaaaatacac ttaataacac aaatctaaca tctaaaaaaa ttgtacaaga tgaggaagta
     1081 gaactttctt atgaagacct tgcaaagtta tcagcttctg ttgttatgat tgttgtgtgc
     1141 aataacgaaa ataaaccgtt taagagcggt tctggagtag taataaataa tgaaggttat
     1201 atacttacga atcttcatgt agtcaatgat gggtattcat ttttagttag atttgaaaat
     1261 gatgataaag tttatacatc ttaccaaatt ataaaatacc attcagatta tgatttagct
     1321 gtgataaagg ttgatagaaa atgcaagcca atacctgtta aagtcagtaa aaaacctgtg
     1381 agaggtcaaa aaatagttgc aattggaagc cctctaggtc tttttaatac agtttcagat
     1441 ggaattattt cagcttttcg tgattttgaa acggttcaaa tgatacaatt tacagctcca
     1501 atctctagtg gtagttcagg aggagctctt ttagatatgt ttggaaactt actaggattg
     1561 atttccgctg gatatgatga tggacaaaac ttaaatttag ctgttgaaag tagtcttgtc
     1621 aaaatatttg ccaataattt tattgagata gtaaactaat caatcaaaat tctacttatc
     1681 ctattccata ataatcaaga gggtgaaaac attgcatgct tttgccctct gattaatatt
     1741 gaaaaaagta ttaatactcg acatttttat gctcaataaa taattgatgc catagacaaa
     1801 atgtaaatat agtccatatt tttctcgaat tatctctatg acctttaaga tgcgtatcta
     1861 aaagttttat tgcatatttt ttatctataa attcatctac atttgaatta taaatagttt
     1921 cctttacaat atcccctaga gaatctttta accaaactct tattggagtt ggaaatccta
     1981 attttttcct ttgaacaaca tgttctggga ctatttctct aaacgcctct ctaagtaaaa
     2041 cttttgtatt attcttattt attttttgct ccattcttaa attttttgcg acatcaaata
     2101 catctttata taaaaatggt actctaagtt caacctgctc agctgtagaa agtttgctaa
     2161 ttttttgaag tatatcgcct tgaagccatg tattcacatc tatatgttgc attttactta
     2221 tataatcgta attatttttt tgtgcatctc tatacaaatt agacaacagg tattcatata
     2281 tattcttttc cttatactta aaaaagaatc tttttacttc gctgttttca aaaacttttg
     2341 catttcctat atatcttttt tctaatggtg ttgtagctct ataaagataa tttcttcctt
     2401 ttatgtttgg cataagttct gaaactcttc ctaatatact tttaatataa gtaggtgaat
     2461 tgacaactga tttcatagta ctatactctt tatatatatt atatcctcca aataattcat
     2521 ctgctccttc tccagacaat gctactttaa catgtttacc tgcctcttta attaagaaat
     2581 aaagacctac ttctgaaggg tctgctatag ggtcatccaa agaataaatt acattcggta
     2641 atgattttat atattcatct tgtgttatat taatctggat attttctata cctaacttat
     2701 cagctgtttt ctttgctact tctaattcac tatatccttt gacactaaag cctgcagaaa
     2761 aagattttat atttggatta atttgtgaag ccacagtagt tataatagat gaatctattc
     2821 caccagacaa aaatgttcca atttctttct cctcttccat atgagttact attgaatcta
     2881 ctactatatt tcttatatca tcctcattca catctctatt gcctaagtga tttttagtat
     2941 aaaaaaattt tgtactactt ctaaactcaa gtttattata tctctttaaa gatagtatac
     3001 cattttctac cctaaaatag tgacctgctg gtatcagcct tattccttgt atcatagtat
     3061 cttcaggaag tacatattga aatgaaaaat aactttgtaa gcttctttca tttatattta
     3121 aatttttcat atattcaagt aaaaacttat actctgagga aaaaattatt gcattttcct
     3181 tatttatata ataaagtggt tttatcccaa ataaatctct tgctccaaat atactttcat
     3241 tttctctatc atagattata aagttgaaca ttcctttaat cttatgtaca caattcttac
     3301 caaattctat ataagatgct aataagactt caatatcatc atttgtagta aaattgtgtc
     3361 cttttttaat taattcttct tttatttcat gatagttata tatatttcca ttaaaaacaa
     3421 taacttttcc atctttttca aatggtttat ttccataaat agcatcaatt acattaaatc
     3481 tttcaaaatc aaaagttttt tcctcatcct tatactctat tctactagaa agtcttgtat
     3541 tatttacaga ttcttttatt ttaaatttaa tatcttcttt tttagatata tcacctttat
     3601 aataaattga aatattgccg tccatataat caaaattcct ttctgtcctt tagtcttatt
     3661 ctatatttca tagttttgtc tttggcttta aaacttattc aatttaaaat gaaattgcta
     3721 ttaaattctt ataaattatc tgttaatatt tcatattttt tttatttcta gaaatactat
     3781 attaggaggt gatttttatg aagaaaaaaa tattaatacc agttattatg tctttattta
     3841 taatctcaca gtgcataact tcatttgctt ttacacctga aaataacaaa tttaaggtta
     3901 aaccattacc ttatgcatat gatgcacttg aaccttatat agataaagaa acaatgaaac
     3961 tgcatcatga taagcattat caagcttatg ttgataaatt aaatgctgct cttgaaaaat
     4021 atcctgagct ttataattat tctttatgtg aattattgca aaatttagat tctttaccta
     4081 aagatattgc tacaactgta agaaataatg caggtggagc ttataatcat aaattctttt
     4141 ttgatataat gacgccagaa aaaaccatac cttctgaatc tttaaaagaa gctattgata
     4201 gagactttgg ttcttttgaa aaatttaagc aagagttcca aaaatctgct ttagatgtct
     4261 ttggttctgg ttgggcttgg cttgtagcta ctaaagatgg caaattatct attatgacta
     4321 ctccaaatca ggatagccct gtaagtaaaa acctaactcc tataatagga cttgatgttt
     4381 gggagcatgc ttactattta aaatatcaaa atagaagaaa tgaatacatt gacaactggt
     4441 ttaatgtagt aaattggaat ggtgctttag aaaattacaa aaatttaaaa tctcaagatt
     4501 aattaaatat taaaatcctt ttgaaaaaaa cttatgtttg ctatataata taataaaaga
     4561 tagtctaaat ttaccaatat aatgtttata gtgacgattc aggaggattt ttatatgata
     4621 aaagaaacac atgaaagggg aggttatata ttcgcacttt tgatactccc ctttataaac
     4681 aatctatacc tagttaaata cgatattgca tataaaatag tattaattat aatttatatt
     4741 tattttgcat atttaggttc tttatttcca gatatagata tgagaggttc ttacataagt
     4801 aaaaaattta tgttaatata caaattattt ggaagtaggt ttagacatag agggtttact
     4861 cacagtctaa ttgccttatt acttatttat agttttttta aatctctgac aatcttcact
     4921 aacaataata tagttttctc atgtttgtcc agtgggttta taataggata tctatctcac
     4981 ttgtgtcttg accttattac taaagaaggt attgaattgt tttatcccat taccataaac
     5041 atttctttac tacctataaa aactaactca aaaacagaaa aattcatatc taagctatta
     5101 aatttcatag ttattttttt gattggatat caattttata ttttattata gttgtagaaa
     5161 aaataaataa caatattttg aactaagtat aaataagttt tatatatttt agtttacaaa
     5221 agaactaaaa attttgtacc ttaatacaaa aaataaaacg tatcttaaaa taatttagta
     5281 ctaaatcatt ttaagatacg ttttatttcg tacaagctaa atcattttat catctttatc
     5341 attgtgatgt tttttatgat ggaaatcata attatgattt tcaagatttt tatttactct
     5401 atttccaatc ttccatgaca aaaacaccaa taatataaat gatgctatct ttaaaggact
     5461 tgttataaaa ccttctatat cacttgccac gtaacttata actataaaca ttacaaattt
     5521 tcctgccaac attgctggca caaaatcttt taaatctttt ttaccaaaag ctgatgcgat
     5581 agtaactaaa caaccgggca taaatggaca ggcatacgct ataaataata acttaaatcc
     5641 ctgtttatcc atccaactta ttgctttttc agttttacta tttctcaact tattaaaaag
     5701 cttactatca ttaaatctgc ttattaataa gaataatgaa gttgtcccaa gtcctgagcc
     5761 aatccatgat aacaaacatc caataaataa tccttgtatt gcagcatttg ctgtaactat
     5821 agcaacaagt ggtaaggctg gtataaagct ttctataaat gcacttaata agccgattat
     5881 tgtggctaac acccaataat tttgagtcaa atcaagtagt tcttgtatat actgcatatt
     5941 gcttttcccc ttttttaact ttaactatta ttatatactc tatatgttta ttatatacca
     6001 atctcaaaaa atgaatctaa tattaaaaag taaataatct atataagttt acagttaagg
     6061 tataattatt ttattatata ttataacaaa aaaggtgatg tttcaaaata aaaatctgaa
     6121 acatcacctt tttataactt aaattgtcat gtaagttata aaaaatatat attagacata
     6181 atattactaa aaatgtctag tcagagtaaa ataaattaca cagtacaaca aatattacta
     6241 tttaattgag ttacttcttt ataacattta accttcttcc tactctgctc aataattcat
     6301 tcgatatact acatgagtta tttgcaactt cagaagcaga taattcagat aaattatctc
     6361 ttcctattat tattgccgta ttaccaactt ctacattagg tatatctgta atatcaacag
     6421 caagttggtc catgcaaatt cgacccacta ttgctgctct acaaccattt attattacgt
     6481 aactttttcc acaggataaa tttctaggta taccatcggc atagccaata gataatatag
     6541 caattacgct atctctgctt gcaataaaga ctcttccata tccaaaacta tcatcttttt
     6601 gtatttttct aataagaatt acttgtgatt ttaaggataa aacaggtcgt aaatcaagtt
     6661 gcaatttagt cgtatcattt ggagaactta atatcccgta taatgcaatt ccaattctta
     6721 cataattaca cttaagattg ggataattaa aaaaaccata actactttga atatgtactt
     6781 taggaatttt tatacctttt tttaacagaa aatcaattaa cttataaaaa cattcaattt
     6841 gtttatttgt aaagtctaca tcttcatctt tcagactatc tgaaacacac aagtgtgtaa
     6901 atatgccaca tatatctaag tattttaatt taaatgcctt taaaattgac ctcacatcat
     6961 taacatcaaa acccagtctg tgcatacctg tatcaatttt aatgtgtgat tttatgttgt
     7021 atttctgttt gtttaaagag atagcatgat taaggtcaat aacactctgt gtaagattgt
     7081 atttatgaag ttcctttgcc cttattgggt tagtatatcc aagaatcaat atttctccta
     7141 gtattccata tcgacgtaaa tcaatcccct catctattgt tgctactgca aatgctttta
     7201 cacctatttg gtttgcacaa gtcgctactt caaaagcact atgaccatag gcattagctt
     7261 ttacaactgc catcaattca caatcttctg gcatagcttt ttgtatcatc tttacattat
     7321 gttccaaatt atttaaatca atttcaatcc aagctctatt ggtatttacc ttatgttctc
     7381 ctttcaagtc tttttttaga ggtttaagaa tcaatataat aattatagaa gctataaaag
     7441 atgctatact taccactata taatgaataa ggctattgtc aaccaagaca gtttgtattc
     7501 cgaatatttt agaaaacata cgaataacaa caatcatcat aggatgtata atataaataa
     7561 ttaatgatga tgttcgcaat aacctagctc gagaaccttt caaaaatgtt aaggctgtaa
     7621 ataagaaata catacatggc aataacataa tatacatgct atcatgacgc tgtaccccta
     7681 gtttatgtaa aaacattccc tcacataaca taagtaagaa tgatataaga aatccaatca
     7741 agctattttt agttgacata taattagatt tattagatat tattccacca agtatgaaaa
     7801 atacaggtgc aaaaaatata ccatttcgtg tataatctga tacttcaaaa atatacccat
     7861 ataaattctt taatagtggt attttttcta aaaaaccata ataactatca ccaaacatac
     7921 caattacata aataacaata gtgattacta gtgagtactt taaacctagt ctttttacaa
     7981 gaaagtatgc aatacttgca ccaataattg atgcaggtag ataccataaa tgatataaag
     8041 tcccatcaaa tacaatgtcc ttaataatat tgggcactaa attattcatg gaaaaatatc
     8101 cagtgtaaat atttaaggga atataaatta caatagctat cccgtaaata aataatacat
     8161 tttttagaaa agatttcagt ttattacaat tataattata tgttgaaatt agaaaaaagc
     8221 ctgatagcat aaaaaagaat ggcacagcaa tacgagcaat tatactagtt aacacaaagt
     8281 cagccatttt actatatgat aataatggag atgtatgaat tgcaattata agtaatgcag
     8341 ctataatcct aaaataatca agccctgcat aagatttatt cctaatcatt cttgttcctc
     8401 catttcttag tcctttctat ttctccaaaa tgtcacaata aaaccatcct tattgttacc
     8461 cgatatttga tatacacatt gttcaggtat agagattctc acttcttctt tagtatgaat
     8521 aggcacataa aagatttcta taattttttc tttatcttct atttttaaac aatttgtata
     8581 cgaaaatctc ttaatatatt ctatatattc ttcaagagcg agattcattt tattgataat
     8641 ctcagaatgc ggatatccca cataacgaaa atgccatggt tcttgagata ttcctgttat
     8701 ttcttctttt ttacactgat aacgctctat aaaaccataa tgtagtgctg ccttacgaaa
     8761 ctcattacaa ataccattat aaggaaattc agggcagata aaatctataa cttctttctt
     8821 taaccctaaa tcaatagcaa gccctgtctg atgttcactg tgatttggaa gtgctacaaa
     8881 ctttttagta aagtcttttc cattgtcacg cagagaactt gaatatatat cttgttgttc
     8941 ctctgcagta cgatatccac ttacaggtac tatttcattt attccacata tactttttaa
     9001 aatattttgt aaaatgtttg tagcttcacg tttcataaga atacttggaa accttgtatc
     9061 tacaggaatt aaatctatat cttcagatgt tttaactggt aaaaaagcat ttactagaat
     9121 tagattccca tcataaactt tatctttagt caataagata gtatttgtca ttttatcttc
     9181 tttattttta tctaataaaa tattattcat cattttatgt acacctcaat tatcttgtcc
     9241 aatatttctt caaaagacaa tccaatacct ttcatcatat ttggataacg actatgagaa
     9301 gtaaagccag gaattgtatt gacttcatta aaaataattt caccagatgg agttaaaaac
     9361 atatctactc tagcaaatcc agaacatcca agagttctat aaataatttt tgctgtctct
     9421 tgtattcttt tctcacaatc caaatcaatt ctagctggca tatgaatttt tgaacttttc
     9481 aatgtatatt tttctgtata atcaaaaaat ccttttgaca attctatttc atctactctt
     9541 ccaacaagta agtcatcgtt tcctaaaact gcacacccta cctcaaaacc atcaatattt
     9601 tcctcaatta ttacttcatt atcatgttca aaagccaagt ctactgctac ttgtaactct
     9661 tcttttttat aaattttact aataccaaat gatgaacctg cacgcacagg ttttataaat
     9721 aatggataag ttaagtcttt tgacagttca gccacattaa atttatctga gtattttagt
     9781 aatattgatt ttggtacttt aataccttct gaatgaacaa gtttatgtga tttgtctttg
     9841 tccatacata atgctgaaga aagagtacca catcctataa cctgtatacc tgccaattca
     9901 aataacccct gcactgttcc atcttctcca ttttttccat gcagtacagg aaacacaaaa
     9961 tcaagtttag tgtatttaat atcattttca catgattcaa tagcactatt tggaatatga
    10021 ttctgagaaa cagcatcagg aattaaaaat tcaataatac catttttgac acggttctgc
    10081 gaaacaataa ctggaattat atcagtatcc ctttcaaacc aagtgttatt ctggatatta
    10141 ttataatctc caatataatg ataccaatta ccctcccttg taattccaat tggtataata
    10201 tcatattttt ccgtattcaa attctcaagt actgcaaaag ctgattgtaa tgatacatca
    10261 tattctgttg agtttccacc aaatataact gcaatttttt tattattcat ctttgattct
    10321 cctttgttga tttatttatt tttatacaat ctacagaaac atgttaaaat actttttcta
    10381 tttcaaatcc aatcaacctt cacttacaat tgcatctgtt ccacataata tattttcaca
    10441 tttttctatt gatataatta ctgtatcaaa ataaaagcac tcttgttttg atacagtaat
    10501 tatatcaata acaagagtgc tctttattta tttatcctaa agaatttcct atttttttat
    10561 acttaaattc ttacaaattt cctacaatcg gaataataac cttaaagata attatattgt
    10621 tctcgctatg agctgttatt gtcccgttat gaagctccac tatttcttta gctatagcaa
    10681 gtcccaaccc agctctacca ctgtttgaat tacgagatgt atccaacctg taaaattgtt
    10741 caaaaatacg ctcaagtttt tcctctggaa tcgtattgct atgatttata aatttaagct
    10801 ttatataatc atcctcctgt acagctgtta tttcaatagt agtatcttca aaactataat
    10861 aaacagcatt tcgtaataag ttatcaaata cacgttgcat tttattaaca tcacacctta
    10921 tcatcatatt tggtgtaaca ttcagtgtat attttaaatt cttactcatc aaagtaggtt
    10981 taaactcata tgttagttgt tcaagcatac gagttaagct tactgtactg tattcaagtg
    11041 atatatttga taaattaaac cttgtaattt caaagaattc attaattaaa tcttcaagac
    11101 gctctgcctt atccaacgaa atagataaat atttttttcg cagttcttca gaaatctgac
    11161 tttcatctcg aagtagtgtc agatacccta taactgatgt aagtggtgtt tttaaatcat
    11221 gtgcaagata aacaataagt tcattcttac gttgttctgc catttgtgtc tcaaatttac
    11281 gttgttcaag tatatgttta attgagttta ttttcttttc tattgccaat aactctggag
    11341 ataaagccac ttctccaaca tcttctttga taagagagtc aataccttga tttatttcac
    11401 taaaatattt tgtaaaccaa tttaaataaa cccggaatat tataaaaaac acaatcacca
    11461 tagctagtat catgaagagc tccatgctat tacggaaaat ctgctgatat aaattcaatg
    11521 cagctttata ttccatacga aaagcatttt caaagaatga tactatccag atagaaaact
    11581 taccatacaa tataaaagaa tataaaatcc atatgaaaat aatggctata aatgccatta
    11641 aagctgttct cataaaaatt ttatttctca cttgagtaaa gtcaggattg actttaatat
    11701 ttttactttt caattgtata acctactccc catacagttt ttataaattt cgggttttta
    11761 gaaggttcat gaattttttc acgtaatcgc cctatatgag ccataactgt gttattatta
    11821 tcaagatatt tttctcccca tacagcttca aacaattctt cagaaggaac cacttttcct
    11881 tgatgttcac ataaatacca aagaatagaa aattcaatcg gtgttaatgc tacttctttc
    11941 ccaaataaac tacatttatg tgtttcttta ttgataatca atcctttaat atcatactca
    12001 ttaacaatta tcgactgttg ttcataagaa ttattatatc gcatatatct tcttaattgt
    12061 gtctttactc ttgcaacaac ttccaaaggg ttaaatggct ttgttatata atcatcagca
    12121 cctatggtta aacccattat cttgtctata tcttcaattt tagctgttaa cataatgata
    12181 ggataatagt atttttctcg aattttctgg caaatatgaa atccatcaat atcaggaagc
    12241 ataatatcta aaatagccat atccaaattt ttagattcaa tacattctag agcttcctta
    12301 cctgtataaa acttgtatac tgtaaaacct tcatttttta aatatacttc aattaaatct
    12361 gctatttctt tttcatcatc cacaatcaaa atattttcgt tcatagatta tttttcactc
    12421 tccttattat cgaggtcatc atttataata ttggtaaatt tacatatcaa ataattacaa
    12481 gcatatttgt aactaatttt tataagaaaa gaactactac atcagaatgt gttgatgcta
    12541 ctattggaac acaaaaactg agattaattg ttattataaa aactccccta acacgtcctt
    12601 attatcgcat aacaggtatt aactttcaat aaaaatatct atcagtcaaa cacaattcaa
    12661 tttattatac cagagacaat ccatataaaa aacaattaaa cctatgtatg taaccacaga
    12721 cattactaaa atttccatgt tattttatat aaaaattcaa aaaaggagtt gaatttaaaa
    12781 tttgattaga aaaaaatcaa ttttgagaca actccttttt attttaatgt atttaaatta
    12841 tagtatatca gccttcataa tatcctcaaa atcacctttt tcagatatta atcttatcaa
    12901 tttaacagta cagcttaaat catttataag tatagctcca actaatgaac catctttcat
    12961 aaataatttt ttatatattc tttgaccttc atcttttgat gaaatttctt tatcaaaatt
    13021 ttgtatatta ccaatactaa atacttttac attcattgca tctaatgaaa ctggatatac
    13081 ttcactttca aatgtcaaat tatctccact tgcatttgcc cctgctattt ttcccatttc
    13141 tactgaagat ggccatattg ctaaactaat atttcccact tgtgcaacat ctccacaagc
    13201 atatatatct ttaacatttg tctgtaatgt cttatctaca acaatacctc tatcaatttt
    13261 tacagatgta tcctgaacca tttgagtgtt tgctcttaca ccaattgaaa atactaccat
    13321 atcacagtcg acactatctc catctttaaa tacaactttt tgagcttttc catcaccttc
    13381 aataccatca actgctacac ccaatcttac atctattcct gtatctctta cacattgttc
    13441 taatatttta ctaccttctt tatcaagttg ttttgatagt atgctatcca tggcttcaac
    13501 tactactact tctaatcctt ttagtctaaa ttcccatgca gcttcaagtc ccaataaacc
    13561 gccaccaatc actacaactt tcttagattt ttctagtgct tcttttacac tatataaatc
    13621 ttttatattt ctaagagtaa acacattttc taaatcatgg cccttaattg gtggtataaa
    13681 gtttctactt cctgtagcta ttacaagctt atcaaattta atagctccat cattcactat
    13741 aatagtttta ttcacttcat ctaatttatc tacattcgtt cctaatatta ctactatatt
    13801 atttttatca taccaatctt tatcttccat gtagaaatct gaaccaagtt cttcatttat
    13861 accatcagaa agtgcaggtc tatagtaagt caactcatct tcattagaaa tcatagttat
    13921 cttacaagtt ttatttcttt ttcttatagc atctgctgca taaaaaccag ctgctccatt
    13981 tcctactatt acgaaatatt catcagtatc ttttctaaaa gtaacttcat cttcttcaac
    14041 ttctataaat tggtcttcct tagctccaca tacagggcat acactaggag cactatcacc
    14101 ttcaaaaact tcattacaaa ttacacattt aaatttctta gttttctttt tacttccaaa
    14161 agtagaattt aacttatcta ggaatttacc tgtataagaa tataatttat caatctcttc
    14221 atttgatggt ttaaagttaa cagcaaaagg ttttactgct gtcattctaa gctgacttat
    14281 tctctccata gcattttcta taccttctcc actccatcca tatgaaccaa atactgaagc
    14341 aacttttcca ccatgaagaa ctgggtttaa cgatattaat atatcccata taggctttaa
    14401 cgcatctcct aatattgttg gtgttccaaa taatacacct tgtgatactg ctatttttgc
    14461 caaaatatct tcttgcttat gctctattac atcaaataaa tttactttat agttgctatt
    14521 tttttcaata tatgctttta tagcttctgc cataatcttt gtatatccat gtgcagaaac
    14581 ataacaaatt gtaacttctt tttctaactt tatttgttca tttacactcc agttgtaata
    14641 aaggtctata atttttctag gattttcagt taatactgga ccatgacctg gacatacagt
    14701 atctatatct aaatctttta tcttttctat agcagttacc atagatggtt taaatggacc
    14761 cattatacaa tcataatagt atcttaaagc atccaagtaa tccttttctt cattttcatc
    14821 aagtgtattt actatcttat cattactata gtgactacca aatgaatcac atgttacaag
    14881 agttttatcc tcttttatat atgtgtatat ggtatctggc caatgaagca taggtacaga
    14941 gaaaaattct aaagttttgt ccccaattga taatgtatct ccatctgtaa ctgcaacata
    15001 ttcaaagtct ttgtttacga tttccttaag atatttaatt gctgtttctg aagctactac
    15061 ttttgcattt ttagctaaat ccaaaagctt ttcaacgctt cctgcatggt ctggttcagt
    15121 atgacttaca acaatatagt ctattttttc aaaatctata tttaaatcat ttaatctttc
    15181 aatataattg tcaaaatgct tatctttaac tgtctcaaat aaaactgttt tttctgttcc
    15241 ttttaataca tatgaattat aagtagttcc ataaggtgta tacataatta tgtcaaatac
    15301 tcttaaatca gggtcaagcg aacctaccca atataaattt tcttttattt ttagtgattt
    15361 caatgacata ccgtactcct cctaaaatag ttatttagtc tctttatacc ctgcttgtta
    15421 tattttaaac aaaatttatt caaaaaagaa attattttaa agatagttta aatcttctaa
    15481 tcttcttttt ctaccttcaa tacagttccc tttttagaat ctacttttat gtcatattca
    15541 tttttatctt tatctattct tattttataa aaagtaacat ctaaatcttt gtctaaactc
    15601 cattctttaa ctattccatt ttgttctttt aatgcaattt ccatagcttg ttgaggtgtc
    15661 attatgtcat ttaaatctaa tttttctttt ctagcaactc catttaaatc ttctgaatcc
    15721 aacttttctg ttttatcttc taatacatct tttgtattag catcaatttt catcttatat
    15781 tcattattat catctacacc ttccacagta taaacaaatt tatttaaatc tctctcaaga
    15841 cttaaatcta ctatatcagc atccttatat ttatctttaa atatgtttat tgcatctgtg
    15901 tatggcactt taaaatcttg ttccattaaa ttttcattat ttttttcatc ttttaaagcg
    15961 ttatttgatg catttgcctc cttttttgta ttatcttgtg cttgtcctga atccttagtt
    16021 ggactcccac tacatgcagt taataaaccc gctataagca tacttaccac taataaatac
    16081 gtttttttac acataaaaac cctccttaaa tatattaatt tattctatat tatgccctct
    16141 aaacccttta tttacccttt tatattaaaa attcctccta ttttatcaat tttaaaactt
    16201 tatatttact aaaattaaag ttacataaaa attatactgt ctcaaaatgg aaacaagcct
    16261 ctttttcatt gttgaaataa taaaaatata ttttatttcg tacaaaaaga gtgcctcatg
    16321 aactaaaaag ttcattttga gacactccca tttttttata tttttactta caaaataaca
    16381 gtacttatta attttttaca aaccttataa gtatatctat atgatattct taaatttaaa
    16441 cactatatta aaaaattaaa ttttgtttaa ttcttttacc gttaaatcca ttttaggata
    16501 tggaatcgta attccttctt cgtcaaacct aactttaacc tcttctagta aatcaagata
    16561 aattgtccaa taatcttctg tcttgcacca aactctagtt gcaaatttaa tagcattatc
    16621 tccatgttct actactccta taaatggttc tggttcattt attattaatt tatgattatt
    16681 aacaatatct ttaagtactc ttctaacatg tagtatatca tcttcatacc ctacactaaa
    16741 agtcaaatca actcgtctag tattctttgc tgaatagttt attagactat catttgatac
    16801 agaaccatta ggtataagta tctgtttatt atctggagta accaattgtg tataaaaaag
    16861 acctatttgt tcaaccgttc ctccatgacc tgctgcttct acgtaatctc ctactttaaa
    16921 aggtctaatc aatagtataa taaatcctcc agcaaaattt gatagactac cttgtaaagc
    16981 caaacctatt gctacccctg ctgatgctat cacagcagca aaactggaca aactcattcc
    17041 ccaatattcc agaacaatga ttataacggc aactttaagt acactcaaaa gaagagactt
    17101 taaaaacttt cttaaagtta aatcaatatc tcttttacta agtaataaca taacatggtt
    17161 tacaaatttt tttattattt taaatccaat agaaagtata agtatgccta ttataagttt
    17221 tactccgctt gtagtagccc attctagcag actttccatt atacctccca tatccatctt
    17281 attaatatct aaattattta aatctaactt gcttatatca tctattttcg tcgtatccat
    17341 aaaaaactcc ttaccttata ttttagaacc aatattatag tttaaagttc ttattttcta
    17401 tttaatataa tacaatacca tttgtacata gtaaacaaga tttatattcc aattatacat
    17461 attaggaaaa tgcaaactat ttgttatata tatgcaaaat ataatactaa attaaactat
    17521 taataaaaca ctatatagcc ttaaaatcat cattttttac ccctatatga gatgatacta
    17581 tagttataat acttagtact ataaatacta caaaaccttt tttatatcca tcaactatat
    17641 tatatgaatt tacttgtaaa gagatagcta taatcaaatt aataattcca cttcctataa
    17701 attcaaaagt attaataaca ctagttgcca gtccagaata aatttcttta tttttatact
    17761 tggcatcatt aaaaacctgt agatgtgaca taactataca tcccataata aagaatgata
    17821 ttggtaaaac tataattggt ggttttacct ggtatacaac tacaattaca atccatataa
    17881 acaaatctat cattgctcca aattttatta tattaaattt gctagacctt attttagcaa
    17941 atacaaaatc cataattatt gaaccaaata taaatccata tgtaaaaaat gatactataa
    18001 acgcagaaaa actcttactc acaccataaa cgtccattat gtatcttact ccccacaagc
    18061 ttataaatgc acttgtaaga ccaacaaaag agaacattat cattgaatta taccatgttg
    18121 atttgttctt tataacagac ttaataccat caacaatatt aaccttctct gacttttcat
    18181 agggttcagt atctacattg aatccatact cttttggtgt atctctaaca ataatataca
    18241 taaaacatcc aattacaaca ccaattatac cgattaataa aaatgaattt ctccaaccaa
    18301 caagttcaga caaaaacaca agtggaaaag tagcaagaac tccacctaaa tttcctacaa
    18361 atgaaaattt agctgttgca gaagcaaatt ctgacttatt aaaccatctc ccttgaattt
    18421 ttaaaataca aagtaatatt actgatgttc ctgcccctac aattacacga gaaatatatg
    18481 ctaattctat actttgaatc aaaccaaata aaattgaccc taaacttgcc attattattc
    18541 caagactgct aatttttctt gctccatatt tatcaatcaa aattcctgca ggtatttgca
    18601 taaatgcata tgcatatagg caaaaggatg ctatatttga aatttgtata gaattaaaac
    18661 ctaactcatt tgctaaattg tctgaaacaa cagcagttga catccttaaa aataatacta
    18721 aaacaaatga aaatgtaatt ataccccaaa cactccactt agcctttgta taacttttca
    18781 tatcttctcc ctcaaatcca tatattttta aattttcttg atataactta tataaagtgt
    18841 atagttattc ttacaaattt ttacacgcaa tatcattata caacatatgt atatatttaa
    18901 ctagaaataa aacactattc atattatata catgaattaa ctacataaat aataagagcc
    18961 atcttttaac attactcaaa agatagctcc atataaaaac atttattcta aatttaattt
    19021 tttatctaat ataaattttg tagcgaaaaa taacgctatt gcaactacaa gactaccaag
    19081 tattgcaagc ataaaactag gactctgata aagatgatat accatatttg tatcttcatt
    19141 tggtaagatg cttccaactg catctccaac aattgatatt actatattta tagctataaa
    19201 agaagcaaat gccactatgt ttctatgttt attgaatttt ggcaattgtc ccattgagat
    19261 tgatgtatat aataacaaaa taaatgatga atatgaaata aacattacta ctactaataa
    19321 taaaatatct aaaaagtttt cagaaatcca tttaaaacta gtgttaaaca aatctaaaat
    19381 ttcaggtagt aaaattccag aagtaccaaa aagcataaga actccaaaag tgaatactgc
    19441 aactataaaa ctaagtacag cgtatataag tgctgttata catttagata atataagtga
    19501 actagtgcta acaggaagag taaacattaa ataaccttca tcccccaaaa gattttttct
    19561 aaacctttga attgtaacta caatagttaa aactcctaat gccataaaaa gacttgttaa
    19621 tactagtatt cctattccct gtacctgaag tatatttgta ttcataaata ttccattaaa
    19681 tacagctact atcaatatgg ctatatataa cggtataaat attcttccac ttgcttttaa
    19741 ctcatatttt aataatttac ctaacattta aattcctccc taaataatgc atctatagat
    19801 tttccttttt cttctcttat tgcttcaacg tctccttgta acactatttc tccttttgat
    19861 atgaatatta tctcatcaca tatgttttct atttcactta ttaagtgggt agcaatcagt
    19921 aaagtactat cttcagaata attttttaat atagtcttaa gtatatatga ccttgcagct
    19981 gggtcaacac cacctatagg ttcatccaat atatatatgc tcgcatttct tgacattact
    20041 aaaataagtt gaactttttc tttagttcct ttagacattg tctttagctt ttcatttaca
    20101 tcaatatcta agcttttaat catctcattt gctcttctaa catcaaaatc actataaaaa
    20161 tcatagaaaa acttaagtaa atctgatacc ttcatccaat catttaaata agttctttct
    20221 ggtaaatatg aaactatttt tttagtatct atggatggtt tcattccatt tatcataatt
    20281 tcaccattat caggctgtaa taatccattc attagcttaa tcattgtact tttgccactt
    20341 ccattaggtc ctaacagccc gactattttt ccctttggta tatttaaatc aatatttttt
    20401 aaaacattct tagttccata actcttgttt acacctttaa attcaactat tccatcttga
    20461 gaaacattgc ttatattttt atccatttta taattctcct ttcagatttt ctgtaataag
    20521 ttttagcatt tcatctctat catatccaag cttttctaag gttactttta atgtatttat
    20581 ctccacatta gctatttcct ctttcatagc ttttattttt tccttatcat ctgttacaaa
    20641 tcttccttta gttctctgac tataaactaa accttgtctt tcaagttctg ttagagcctt
    20701 ttgcattgta tttggattaa cttccgcatc ttctgctagt gctctcactg tttctaattt
    20761 agatcctatt ttaatttccc ctgaaattat ttttaacttt aaatgttcta ctaattgtat
    20821 atatataggt ttgttattat caagttccca ttccatgttc atgcctcctt attatattat
    20881 tgtattaagt tgttaataca ataatataat aatacaaagt atatgtcaat tactttttag
    20941 taaaagtata aaaaatttta caaaaaaaat gaacactaca ttagagtcca tttttaacac
    21001 aatttattaa attctttata ttacagatta attaccctct taccaagtta gatacaatta
    21061 aatgttcaaa ttcttcaatt ggcattggct ttccaaacaa atatccttgt gccatatcac
    21121 actttacttc ttttaaaagt ttaaactgtt cttctgtctc aactccttct gatatgactt
    21181 tcatacccaa ctcttttgcc atttctatta ctttagtaac aattattttg cttcttttac
    21241 tattataagt ttccataata aatttttggt caagttttaa gatatccata ggaatttctt
    21301 ttaacatatt taatgatgag taccctgaac caaaatcatc catagatatt aaaaatccta
    21361 ttttttttaa attattcatt atatcaatta aaatatcaaa attatcaaat atgatactct
    21421 ccgttaattc aagttctata tactttgcag gtatattgta tttatcaatt atatttttgt
    21481 atgtttctat aaaattattg caatataaat gtatccttga aacattaact gaaacagtaa
    21541 ctggatttat tcctgcatca atccattttc taaggtatat acatacttct tcaagcacat
    21601 ataaatctaa atttataaca aaaccgtttc tttcaaaaaa tgggataaat tcatttggag
    21661 gtattagccc tttatcagga cgtttccacc taacaagtgc ttctgaacct tgtatctctc
    21721 ctgtattaag ttctatctta ggttgtaagt atataataaa ttctttattt tctagacttg
    21781 aaaacattgt attttccatc tctttttctt ttatctcttg gtcatgaatc ttattatcat
    21841 aaaatgcata tgaactttta tgtcctccct ttgccatttt atgaggtata ttagccctat
    21901 ctatgattga gttgatatct ctatcttctg gaagtataaa atagattcca caacttaaaa
    21961 ctaacttata tttttcttct tgattattat taaacaagtc taattcttca aatatagttt
    22021 ttaacctctt tgttatatct tcttcttcta tatatttcat aattataata aaattatccg
    22081 cagatattct tgcaaacatt tcctcttcaa atatattgct cttaataatc tttgttaaat
    22141 tacgcaaaat ttcattccca acttcatatc caaataaatc atttatatat ttaaatttat
    22201 caatatctat atataataaa gcatacttta cttgagtatt tttacatagt attttgttag
    22261 catcaattac aaatttatct atagtatttg ctcctgtaag attatctgta tatgctatcc
    22321 tttgcaattt aattttaaaa tttaatttat catatattat atagaatata agtattacac
    22381 atattatagc cataataagt attatttgta ttgcatgttt gctaataagc tgattattag
    22441 acacaataga ctcaaagtta tttatggagt tgtttttata gagtttgcat aaagaataga
    22501 ctggtgttgt gtatgtaatt tgtaaaatta tacaaataaa taaaaaaaac atcaattttt
    22561 tttgtaaatt cctcataaaa atttcctccc attaaaacac ctctatttta ttagcaaata
    22621 agtacatctc aaaagtttcc atttcttgta aaatggatag tttatatcta ctttaatttt
    22681 gtaaaatttt ctgctactat aattatagaa tgtagcatat tcattgtctt aactattgat
    22741 ttata
//
LOCUS       DAMJYR010000053        22328 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.053, whole genome shotgun sequence.
ACCESSION   DAMJYR010000053 DAMJYR010000000
VERSION     DAMJYR010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 22328)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22328)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22328
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.053"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            79..1200
                     /locus_tag="PC471_002033"
     CDS             79..1200
                     /locus_tag="PC471_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PcfJ domain-containing protein"
                     /protein_id="HDF2789162.1"
                     /translation="MKSYKEKQFLVFEFENGKNVKYNLATGEYVGKSGRIVKDVCTQL
                     RGYNLHEVINSFEDENYKCFLNFVDERVNRSKSSRAWGRRVERIRNIGSFLNEINNYS
                     EYEQLFSAGLKKIIYPIHCDIKSIPKGLISICRNHSITLSDRLIQGYSKNPNLYNNLL
                     SLELNSIRKTNLISILDSGYDRVIFDRLIDTYKYKPQSLINYIDNLMTYEALNGLEST
                     LNELYDYVVMMSAISDKYEKYPRHFLTTHKIATRNYNRLKTQFEEEVFNKRIDKSLEY
                     SYQDYKFIYPKSTSEIKDEAVQQNNCVSSYIQNVIDGKCHILFMRKKDNPSKSLATIE
                     VRNGKIVQAKGKYNRDVNEREQEAIDKYNNRIERMKKVC"
     gene            1194..1631
                     /locus_tag="PC471_002034"
     CDS             1194..1631
                     /locus_tag="PC471_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2789163.1"
                     /translation="MLIEGTKIRLMKEIQGFGMLKIGDVFVITSVGNNGAIHFKTDYG
                     MGFMSYSEFKEHFEIANNKEKRKYNWSNWTTIVSPNLHILYTYKTNNKKVIVKKDGVK
                     ASATCHDTDVFNLQKGIKICLARIEVKEAEHRLNLLLDEINNK"
     gene            1649..1804
                     /locus_tag="PC471_002035"
     CDS             1649..1804
                     /locus_tag="PC471_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009900651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2789164.1"
                     /translation="MTCKLKDICENKNTDCDYCCYNPNAYLEDAFEWNGEGKEPSSEE
                     LDDALAE"
     gene            1848..2408
                     /locus_tag="PC471_002036"
     CDS             1848..2408
                     /locus_tag="PC471_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2789165.1"
                     /translation="MRWRNILVTSVLVMGSSIHIFANTKEISTSRMDKNIVADTAKNI
                     NLMYKYEWEQHKNSSEYKLEIKRINLEKDLGIKIEKLIPVTWEVSYYTSLNCENSKYG
                     AITATGEKLQYGFVANNHLKFGTKILVDGNLKVVKDRGSNKYFGNSNAIDVFVPKLEG
                     ESDYKYYKRVNNMGRHYKEGYIIVEG"
     gene            2420..3088
                     /locus_tag="PC471_002037"
     CDS             2420..3088
                     /locus_tag="PC471_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009900647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2789166.1"
                     /translation="MNVNGIKIPGIIDVSNVYVYGIKESIIASGYLMYEQQVIDMKKI
                     NISEKDLKRANQLGKSKQGSGHDCFSKGIIIQMDLQWSEYMWRQWDRYHHNDYVSSQS
                     KMHRLSKFNLDDMCNKYVDKNTINFLNELIHEYNNFEELKLNNSNNIILRNGEKVEVT
                     KETLWKIIISNCPSGLMLTARVTLNYLQIKSMINQRENHKMEEWRILCDFFKTLPKYK
                     ELMM"
     gene            3134..4027
                     /locus_tag="PC471_002038"
     CDS             3134..4027
                     /locus_tag="PC471_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2789167.1"
                     /translation="MAEKRINYNQVFVSGEVLDILNIARMNQGTGQEAIRFTLKVETA
                     PNESVNVDYFTSMFKSDGSPNQMFLGIETIANEIKTRAEDGKGDIVRCVCSLDNNLYY
                     KDGEKKERFQISGTFCNREKYDDKGNPIDDKGEVVKSIKASQVWRVYTLIENIEEKED
                     DLGKYLEIKGLINKYGKQGCNMTFRIHNEDMIEGFKSLYKIGDVGLLEGTVKSMVVSK
                     SAGFGSRIKKSAFTFLEIEGGDEPLKDGDKIFSDKNYPFTDKNIEAMREKIKEKDEKE
                     KQRDIDKNGKTIEISDDDVPF"
     gene            4067..4624
                     /locus_tag="PC471_002039"
     CDS             4067..4624
                     /locus_tag="PC471_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ERF family protein"
                     /protein_id="HDF2789168.1"
                     /translation="MEVKEKTMNDGFKKVVNEEIKTYISNLYSDRIDGLKESDSQIEI
                     TKALIAFHLDIESIPKDNTNPFFRSKYANLDTILKAVNPLLAKHDLILMQSAEDAGND
                     KVYIKTKLKHSSGEYIESNSAPFKPAKTNDIQARGALETYLRRYAVQSVLALSFEEDD
                     DGNSLTNKSKGKSEEENSTIKKSRV"
     gene            4685..5236
                     /locus_tag="PC471_002040"
     CDS             4685..5236
                     /locus_tag="PC471_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2789169.1"
                     /translation="MNCKCRFCKKKLNTNDAYKVEHITSGGNKQNRYYCNEQEYRKEQ
                     QDIYFWKQCQLGIDYIMGYTVINNQKNKMLQEIIKGGYTREELYDCIIEKKDEVIELL
                     NYRKDIEEEYPKLCYVFTILKGSIRDITIKNKQMKYEKENERIYKEVEKSEEYYEIIA
                     PKKVIYNKRQSLFDKVKGVYQDE"
     gene            5277..6803
                     /locus_tag="PC471_002041"
     CDS             5277..6803
                     /locus_tag="PC471_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2789170.1"
                     /translation="MVLGTMLKSLTLFSEYKISESDFIIDKVKFFFSLGRIMSKTHNE
                     LDEISVAKFVSSNKLKSEYERYGGWNSLSSAMEYGKETNIAAYIDDLAKNNLLIALDK
                     RGFNVIKEMEHNGLKFIPFEIFQSMKCREVEEFYEGLISSCSVNSIKNNMKVENLLLT
                     KDDREKLKQKTEAGTPYNIIFEYTEKEIGLSNNEESKYIYSLPILSNRTNGIGKGGGI
                     NIIAGFSGIGKTTLLFFNYILAMIYRGEKIVIFANEQKSQYFKSMLVSFIAYNIFNYH
                     DLDRNKIDNGDFTDFEEDLMEKIEKFLRDRCFAENLKFIYMEEFEISEILRKSKELVT
                     HQGFTGIAVDTFKSEDSSDAHYTGKLIENSKLLDSFGNKYNVITMLSMQLLTAQENKS
                     SYLSAGDLSEAKAVKNVCGLLKLMRKVVNELELDSTNKKFFLKPYKLKYNKLKKTTER
                     EYIQFDAKDLQKEYRLLFLNKSRRGQDGDVILLRFYGKTGRLEEIGRCEKVYRGQLSY
                     "
     gene            6813..7874
                     /locus_tag="PC471_002042"
     CDS             6813..7874
                     /locus_tag="PC471_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2789171.1"
                     /translation="MEIKDLTNEQVINFMEYLGSDLSPKSNDRQLIFNTCLCHNGDSY
                     KLFYYTESKTFHCYSSCGHIGSLIDLLIHINKYEFKDAINEIKDFFGISNQPMLRKGF
                     RIKKKVEQIHDIKDIQIELLPTPKKPYVYKTFQQVPIEEWENENISFEVLKTYQVYYN
                     LYENQIVIPHFCWHDRARPVGIRVRNLDEDKAKSFGKYIPLWYDNRCYNHRLSLNLYG
                     LNVNKKSIKNSKKVIVFEGEKSVLQMATMYKNNPSVAICGSNFSREQKKILIDLGIEE
                     LIIAFDRQFKVKDDEEYVIWRDKIYKLVQDIKDVVNISVIWDKYNLLGYKDSPSDKGK
                     TIFEKLLKNRINIENFVKQ"
     gene            7992..8444
                     /locus_tag="PC471_002043"
     CDS             7992..8444
                     /locus_tag="PC471_002043"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2789172.1"
                     /translation="MKIVSISIDLLLKNMCICILDNGHIRELSNRDIIADKFIFEIYK
                     IFNNHNCDKLVLDISGIGIAHYQNLEEHIDKNKLIGVYLAEKNNSNKILMNFLNNESF
                     YNLGLEICVKNNGGRIYPDMSKYDEIERGIIECIMLANYYSNPSIESE"
     gene            8476..8748
                     /locus_tag="PC471_002044"
     CDS             8476..8748
                     /locus_tag="PC471_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2789173.1"
                     /translation="MINFSIKDVHMGDRLYCMNNIGNYKKGEIYTVSDTSFFQLFHKI
                     KGENCLPIDCYFICLPIKEKVETNPKSMTKEEIEDELGYKIKITSK"
     gene            8769..9575
                     /locus_tag="PC471_002045"
     CDS             8769..9575
                     /locus_tag="PC471_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009900629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2789174.1"
                     /translation="MNIFNKKYGRNVKITEAEPPSPPPKQPNFDMDNIQYDKEMKRKL
                     LEMEKIKIDSEMKKNSEEWIWIEGYKGTDRDMKCRNFQYELNKTYSVEEIHRGNINIY
                     DYGFHLCLDLKDVFRCYNLDLSNRFFKVKALVRREDKYRYGMKQSFSSIRPGAKCSIG
                     AKEIILFRELTFEELKQDIQDKFPKINNELEWNLIKKLGIYKYYNKIFINKMKEIGYS
                     DIFSQVLFDEYHPLINPHRTMNILDKAIAYSKENLSKDMIIYLLMKEGCR"
     gene            9572..9724
                     /locus_tag="PC471_002046"
     CDS             9572..9724
                     /locus_tag="PC471_002046"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2789175.1"
                     /translation="MIVFELLIIIFIAILMVTIFLSCLLVSISMIPKIIDEIKDIIDK
                     IKKEKK"
     gene            9726..9920
                     /locus_tag="PC471_002047"
     CDS             9726..9920
                     /locus_tag="PC471_002047"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2789176.1"
                     /translation="MEFKKELEVMEDSEIKFIQSLNRINNNIRINEETKICLEKEIYA
                     SSRVLSMLCDLNEIYSKQNK"
     gene            9955..10614
                     /locus_tag="PC471_002048"
     CDS             9955..10614
                     /locus_tag="PC471_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003401688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HDF2789177.1"
                     /translation="MIKYVMSDLHGMYDKFISMLEQINFNSNDHLYILGDVLDRGDKS
                     LEIIDYIRKYKNITLLKGNHELMFQESYTDVNSSFLWFYNGGKDTFYDLGMKSYEYKE
                     NFYKYIKNLPYLEIIDNFILVHAGLYLPNNYENLSIEQIIELQEEDICVWDRTILNTN
                     KHIKGYTIIIGHTPTQNIKDYEKAEIYKQEGIINIDCGACFDNGKLACLRLDDMKEFY
                     I"
     gene            10625..11152
                     /gene="yyaC"
                     /locus_tag="PC471_002049"
     CDS             10625..11152
                     /gene="yyaC"
                     /locus_tag="PC471_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore protease YyaC"
                     /protein_id="HDF2789178.1"
                     /translation="MDILKISYNNLNAISKISEVLRDIVNEDTLIVCIGSDRVLADSL
                     APMIGSILEKSTIRNKIFGVLGDSIHALNLEQKIQAIKNNYPNSNIIAIDACISKISD
                     KGTIIISNKPVKPGLGVDKKLLEVGDYSIVGIIGRNKYDIYDTSDPELILDLADVISK
                     SLISILLEKEERMIV"
     gene            11149..11403
                     /locus_tag="PC471_002050"
     CDS             11149..11403
                     /locus_tag="PC471_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2789179.1"
                     /translation="MIQEVRQEILLKNGLILYTGDLVEIKYKSDEDVIEHTCKGKIKE
                     AKELFIKLDTSKKYKTSERMIYSWELKEIKKVDDEDEKDN"
     gene            11387..11644
                     /locus_tag="PC471_002051"
     CDS             11387..11644
                     /locus_tag="PC471_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2789180.1"
                     /translation="MKKIINSKYRALYLFIFFLIFNLIYSLYFGRNTEIGFYSSTSCI
                     EEYILDIITSIGLFFSMMLAGFDVTENFINSLRDYCEKDAK"
     gene            11655..11864
                     /locus_tag="PC471_002052"
     CDS             11655..11864
                     /locus_tag="PC471_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2789181.1"
                     /translation="MELKDYISKVELNDMPLTLDNQYMLNEISISEYGKIFAVKDTSG
                     RTSYYTEIEFNKLFRGINSILENII"
     gene            11939..12748
                     /locus_tag="PC471_002053"
     CDS             11939..12748
                     /locus_tag="PC471_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HDF2789182.1"
                     /translation="MEKELYSFSKLNAFHTCPFQYYLTYVKNLNREQNCYGYYGNELH
                     KLLEELQQRKITNQEAIQKYNEVIEYANLMDYNFPTPNSRINYLECILHYIENFVPIE
                     CDKYYIEEYFEFDINGITMRGYIDLYYVIDDKIYVIDYKSSSKFSKKDLPKKSMQLIL
                     YAMYLKEKYPDKVIEYVAFDMCKYMKNEKGVLIERNKIDNINNYERAIVKIKYTKELE
                     KQLIDFVVDTVTQIKQLDFNDESVWCKNDDKSNQFFCKTLCSHYNKGEDLC"
     gene            12742..13503
                     /locus_tag="PC471_002054"
     CDS             12742..13503
                     /locus_tag="PC471_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003372918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HDF2789183.1"
                     /translation="MLIDRGNYKLYKGDCLEVMDGLIKLGVKFDAIITDPPYGTTQNK
                     WDSVIPLDKIWSRINKLIKPNGAIVLFGSEPFTSLLIRSNLKDFKYNWIWQKNKSTGF
                     LNSKKQPLNDHEIISVFYKKQCVYNPQMTKAEKIYNRGYVVRNKDEGIQQSDNYGEQK
                     SFMQVDTGLRYPKRIQYFNNNFTKQQLHPTQKPIDLLEYLIKTYTNEGDLVLDFTMGS
                     GSTGVACINTNRRFVGIELDEKYFNISSKRLSEIS"
     gene            13614..14399
                     /locus_tag="PC471_002055"
     CDS             13614..14399
                     /locus_tag="PC471_002055"
                     /inference="COORDINATES: protein motif:HMM:NF013704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2789184.1"
                     /translation="MIFIEINKVYNIDCIEYMKTLPKECVDLIIADPPYYRILKETWD
                     NQWGTENDYFKWCCKWIKESKIILKQNGSMYIWNWFDNICEIGHICRENDFYIRNLIC
                     WNRGGGRERNNWGSKKEDLLYISKTKNPTFNLNDVLLSADDINRKMSKQAWERGKYER
                     KGRKNYNQESVNPSNVWYDSILTYNSKEKVNHPSQKPLSLCNRIIKASSNENDLVYVP
                     FAGSGSEIISCIKNNRNYIATEINNDYIEEIIIPRIKNLKESV"
     gene            14400..14696
                     /locus_tag="PC471_002056"
                     /pseudo
     CDS             14400..14696
                     /locus_tag="PC471_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895083.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            14777..15349
                     /locus_tag="PC471_002057"
     CDS             14777..15349
                     /locus_tag="PC471_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HDF2789185.1"
                     /translation="MNKIFVFLGYSGSGKDSIVTEISKQFNIPILISHTTRPPRGQSE
                     IINKTYHFVDNKFFKKEKNNFIEMRKYIVHDGSTWLYGIHKSELENKKYALTIVDSCG
                     YKALEKYFMGTKTKLIPFFINTDENILRKRLINRGDNAKEIERRLADDKLKFNDFLNN
                     ESYIAIPNNTNLINAVEQVKIHMKEGIEWY"
     gene            15340..15834
                     /locus_tag="PC471_002058"
     CDS             15340..15834
                     /locus_tag="PC471_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HDF2789186.1"
                     /translation="MVLALDISMSSTGWAVINRNKKVLKYGKIVTKKDKFKSEDERMC
                     YICNTIQELIITHNIQIVLVEDQFTSRNSKTILSLRKLLGAIMRTVKLNNIEIEYMYP
                     VSIRKYLMNNGKAKKEEVATYVRENIIDIGEYIDRTCKAKTSDMYDAISIGIAYLNKL
                     DTNR"
     gene            15858..16361
                     /locus_tag="PC471_002059"
     CDS             15858..16361
                     /locus_tag="PC471_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyuridine 5'-triphosphate
                     nucleotidohydrolase"
                     /protein_id="HDF2789187.1"
                     /translation="MSINVKIKKLNQDAIVPSYAKEGDSGIDLYTLKDTVIPAKSAVA
                     IPTGIALEIPYGYEMQVRPRSGISLKGMFFTRYVTIYDKVQPKKQIRKIFVAHPTVRL
                     GTVDSGYRGEIKIITYNEEDFEILIPKHTKLAQGVFQQVPKAILKVVDELTSSERGSN
                     GFGSTGI"
     gene            16526..16699
                     /locus_tag="PC471_002060"
     CDS             16526..16699
                     /locus_tag="PC471_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2789188.1"
                     /translation="MYRYDDYVNLINDLGLPKEIRNKCVRVIMTLENMQDIMVVHKFK
                     TYVVNEKYIEKVA"
     gene            16820..17569
                     /locus_tag="PC471_002061"
     CDS             16820..17569
                     /locus_tag="PC471_002061"
                     /inference="COORDINATES: protein motif:HMM:NF019976.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009617778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NERD domain-containing protein"
                     /protein_id="HDF2789189.1"
                     /translation="MFKEIFEKVFMSEEVSKTVKEVNNIIKDTKIQVKGGFGELRLDF
                     ILSRLGKDYIIIKDIIVPGSNKTTQIDSIVISMYGIFVIECKNFSGYIYGNDKDKVWT
                     QIVGKTKNTFYNPARQNYAHIKAIENIIGDKYNIYSIIVFSDKATLKNVMISGNNIIV
                     INESEILSTISKCKDVTIDKEEIKIIRDKIFDCMKTTNQNTREHVRNLKNITEEDKCP
                     RCQIGHLIKRKGQYGEFWGCSQYPKCKYTRK"
     gene            17858..18970
                     /locus_tag="PC471_002062"
     CDS             17858..18970
                     /locus_tag="PC471_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDF2789190.1"
                     /translation="MNAFIRKRNKNYVVYLEFRDDESGKRKQKNMGAFDKKRDANKRL
                     AEVKDSIYKDSFLVPNEITLAGFLLDFLEKYKDNISASTYKSYIAICKNHINPSIGKY
                     RLQELRNIHIQNYIDDLAGNLNPQTIKVHINVLRLAIKRAYRIKLIKENIIDGIESPR
                     IKKFKNEIYDKEHMLKLLEVAKGTNLELPISLAIGLGLRLSEVLGLTWDNIDFDENTI
                     TVNKITSRLDGSVILKEPKTESSVRKIFAPIELMNLLKNYRLEQNKKLLRSIVRNEYN
                     LLFFDRKGNPIAEDVMSKKFRKFLENNDLPHIRFHDLRHSHVTLLINSKVPIKVISER
                     VGHSNINTTLNVYSHVLKEMDKEASDRISENLFKAN"
     gene            19547..19924
                     /locus_tag="PC471_002063"
     CDS             19547..19924
                     /locus_tag="PC471_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HDF2789191.1"
                     /translation="MYLRKLSKSELIVMKFIWNLDTKVKSYQIINYMKEKYSWSEKTT
                     LKTLSKLSNKRFIYVQETSQCTYYTISIKEDKYHEFISQKIHKLLGCNSIKSLLASLF
                     QEELTDEKITSLEEWVKNWEEEE"
     gene            complement(20065..21327)
                     /locus_tag="PC471_002064"
     CDS             complement(20065..21327)
                     /locus_tag="PC471_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HDF2789192.1"
                     /translation="MKFKRKALTLAIISSIVMSSTLLVSAKELKFRPPAHRIQGANKY
                     ETAGLIADRRKYTQAIIINTDKSLADGLSASGLAGASNSPILLTKQNSIPNSTLKRLD
                     KFKKIYLIGGVNSISKNVENILKNKKIKVIRIEGKDRIDTSYNVAKEISNLKKVDEIY
                     LTNAYQGEADSISISPVAAKYKNPVVLTNGKNIPFKTDGVKTYAIGGTASINDSLVNS
                     TKATRIGGVDRLDTNEKIIKHFYKDELKHPNQICIVSSDNLIDALLSSTIHKEYPVFL
                     VNETNDKSLVTSANFPIIIGDIKNEILDQCLTPDYMLTGNTKRSDISKALDAIYKAKG
                     SKAHEYCYTYNYGNSRYKKAENIDFPHYEFEIISVKNVKQDESSTDGSDTYTDKEDSI
                     KTVGTLIVNSETLEVYEYSFDTQKLTKI"
     gene            complement(21482..21808)
                     /locus_tag="PC471_002065"
     CDS             complement(21482..21808)
                     /locus_tag="PC471_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009899257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HDF2789193.1"
                     /translation="MSMQKIPQAELKVMKFIWNKNDIVTSKEVMEAMELEYNWKATTT
                     LTLLSRLTIKRFLDSERIKRITHYTILITKEEYKIFETKRFLEEVHSNSIESLISSLE
                     DCYKNK"
     gene            complement(21940..22293)
                     /locus_tag="PC471_002066"
     CDS             complement(21940..22293)
                     /locus_tag="PC471_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009899255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2789194.1"
                     /translation="MLEEDEIYNVIGKRIRNYRRKAGYNQNTLAKKAGLFPAYIGQIE
                     RGESKASLRSIFKIANALEIPLEVLFENIIQNENDVETLSSEAYELIDSLTVKEQKAI
                     IRLIKEIIEYRKFEE"
BASE COUNT         9298 a         2167 c         3663 g         7200 t
ORIGIN      
        1 ttgagaacat agaaggtata tgatatacta tgaatataac aagaacatag aaggtaaaaa
       61 ggaggtagtg attaatattt gaaatcttat aaagaaaaac agtttttagt atttgaattt
      121 gaaaatggga agaatgtaaa gtataatttg gcaacaggag aatacgttgg taaatctgga
      181 aggattgtaa aagatgtgtg tactcaactt agaggatata atttacatga ggtaataaat
      241 tcttttgaag atgaaaatta taagtgtttt ttaaattttg tagatgaaag agtaaataga
      301 agtaagagta gcagagcttg gggaaggagg gttgaaagaa taagaaatat aggaagtttt
      361 ttaaatgaaa taaataatta ttctgagtat gaacaattat tctcagcagg attaaaaaaa
      421 ataatttatc cgatacattg tgatattaag agtataccta aaggtcttat ttctatctgt
      481 aggaatcata gtattacttt gagtgaccga ttgatacaag ggtatagtaa aaacccaaat
      541 ttatataata atttattaag cttagaattg aattcaataa gaaaaactaa tttaatttca
      601 attcttgatt ctggttatga tagagtgata tttgatagat taatagatac atataaatat
      661 aagcctcaat cgcttataaa ttatatagat aatttaatga catatgaagc attaaatggg
      721 cttgaaagta ctctaaacga attatatgac tatgttgtta tgatgagtgc aataagtgat
      781 aaatatgaaa aatatccaag acatttctta acaactcata agatagcaac tcgaaattat
      841 aatcgtctta aaactcaatt tgaagaagaa gtatttaata aaagaataga caaatcacta
      901 gaatattcat atcaagatta taaattcata tatccaaaaa gcacaagtga aattaaagat
      961 gaagcagtac agcaaaataa ttgtgtaagt agttatattc aaaatgttat agatggaaaa
     1021 tgtcatatat tatttatgag aaaaaaggat aatccaagta aaagtttagc aacaattgaa
     1081 gttagaaatg gcaaaatagt ccaagctaaa ggtaaatata atagagatgt caatgaaaga
     1141 gagcaagaag caattgataa atataataat agaatagaaa ggatgaagaa agtatgttaa
     1201 tagaaggaac taaaataaga ttaatgaaag aaatacaagg ttttgggatg ttaaaaatag
     1261 gagatgtatt tgttattaca tctgtaggaa ataatggagc tatacatttt aaaacagatt
     1321 atggaatggg gtttatgagt tatagtgaat ttaaggagca ttttgaaata gcaaataata
     1381 aagaaaaaag aaaatataat tggagtaatt ggacaacaat agtttctcct aatctacata
     1441 tattgtatac ttataaaacc aacaataaaa aagtgatagt taaaaaagat ggagttaaag
     1501 cctcggctac ttgccatgat acagatgtat ttaatttaca aaaaggtatt aaaatatgtt
     1561 tggcaagaat agaagttaaa gaagcagaac atagattaaa tttattatta gatgaaataa
     1621 acaataaata atcttaggag gtctaaaaat gacttgtaaa cttaaagata tatgtgaaaa
     1681 taagaatact gattgtgatt attgttgtta taatccaaat gcatatttag aggatgcttt
     1741 tgaatggaat ggagaaggaa aagaaccatc tagtgaagaa ttggatgatg ctcttgcaga
     1801 ataaataagt atttaaaatg ataattttaa tatgattgga ggtctttatg aggtggagaa
     1861 atatattagt aacaagtgtt ttagtaatgg gtagtagcat acatatattt gctaatacta
     1921 aagaaataag tactagcagg atggataaaa atattgtagc agatacagct aaaaatatta
     1981 acttaatgta taaatatgag tgggagcaac acaaaaatag ttctgaatat aaattagaaa
     2041 tcaagagaat taatttagaa aaggacttag gaataaaaat agaaaaatta attcctgtaa
     2101 catgggaagt aagttactat acttctttaa actgtgaaaa tagtaaatat ggagctatta
     2161 cagctacagg agaaaaatta caatatgggt ttgtggccaa taatcatctt aaattcggaa
     2221 ctaaaatatt agttgatgga aatttaaagg tggttaaaga tagaggttct aataaatatt
     2281 ttggtaattc taatgctata gatgtatttg taccaaaatt agagggggag agtgattata
     2341 aatattataa gagagtaaac aacatgggaa gacattataa agaaggatat attatagttg
     2401 aaggttagga gttgataaat tgaatgtaaa tggaataaaa ataccaggaa ttattgatgt
     2461 gtcaaatgtg tatgtttatg gaattaaaga aagcataatt gcaagtggtt atctgatgta
     2521 tgaacaacag gtaatagata tgaaaaaaat aaacatttca gaaaaagatt taaaacgagc
     2581 aaatcaatta ggcaaatcaa aacaaggttc aggtcatgat tgcttttcta aaggaattat
     2641 cattcaaatg gatttacaat ggtcagaata tatgtggaga caatgggaca gatatcatca
     2701 taatgattat gtgtctagtc aaagtaaaat gcatagatta tctaaattta atcttgatga
     2761 tatgtgtaat aaatatgttg ataaaaatac aataaatttt ctaaatgaat taattcatga
     2821 gtacaataac tttgaagaat tgaaattgaa taattctaat aatattattc ttagaaatgg
     2881 agaaaaagtt gaagtcacaa aagaaactct ttggaaaatc ataatatcaa attgcccatc
     2941 aggattaatg ttaactgcaa gagttacatt aaattatcta caaataaaat ctatgataaa
     3001 tcaaagagaa aatcataaaa tggaagaatg gaggatttta tgtgactttt ttaaaacact
     3061 accaaaatat aaagaattaa tgatgtaata gttttgttag caattaaatt ttaaatataa
     3121 aggagaagtg ggtatggcag aaaaaagaat aaattataat caagtatttg tatcaggaga
     3181 agttttagac attttaaata tagctagaat gaatcaagga actggccaag aagctataag
     3241 atttacctta aaggttgaaa cagcaccaaa cgaatcagta aatgtagatt actttacatc
     3301 aatgtttaag tcagatggtt caccaaatca aatgttttta ggaatagaga caatagctaa
     3361 tgaaataaaa actagagcag aagatggaaa aggtgatatt gttagatgtg tttgttcatt
     3421 agacaataac ttatattata aagatggtga aaagaaagag cgattccaaa tatcaggaac
     3481 tttttgcaat agagaaaaat atgatgacaa aggtaatcct attgatgaca aaggcgaagt
     3541 agtaaagtca ataaaagcga gtcaggtctg gagagtatat acattaattg aaaatataga
     3601 agaaaaagaa gatgatttag gtaagtattt agagattaag ggtttaatta ataaatatgg
     3661 taagcaaggt tgtaatatga catttagaat acataatgaa gatatgatag aaggatttaa
     3721 aagtttatat aaaataggag atgttggcct tcttgaaggt acagtaaaat ctatggttgt
     3781 tagtaaatca gcaggatttg gttcaagaat taaaaaaagt gcttttacat ttttagaaat
     3841 agaaggtggc gatgaacctt tgaaagatgg agacaaaata tttagtgata aaaattatcc
     3901 tttcacagat aagaatatag aggcaatgag agaaaaaata aaagaaaaag atgaaaaaga
     3961 aaaacaaaga gatattgata aaaatggtaa gacaattgag ataagtgatg atgatgtacc
     4021 attttaaatt ataaattaaa taatctatta aaaaggagag atgtgaatgg aagttaaaga
     4081 aaaaacaatg aatgatggtt tcaagaaagt ggtaaatgag gaaataaaga cgtatatatc
     4141 aaatttatat tcagatagaa tagacggatt aaaagagagt gatagtcaaa ttgagataac
     4201 aaaagcatta atagcctttc atttagatat agaatcaatt ccaaaggata atacaaaccc
     4261 atttttcagg tctaaatatg caaaccttga tacaattctt aaagcagtaa atccattatt
     4321 agcaaaacat gatttaatac taatgcaatc agcagaagat gcaggtaatg ataaagtata
     4381 tataaaaact aaactaaagc attcatcagg cgaatacata gagtcaaatt ctgcaccatt
     4441 taaaccagct aaaactaatg atatacaagc tagaggagca ttggaaactt atttgagaag
     4501 atatgcagtt caatcagtat tagcattatc attcgaggaa gacgatgatg gaaattcttt
     4561 aacaaacaaa tcaaagggta aatcagagga agaaaattca actattaaga aaagtagagt
     4621 gtaaataaaa ttgctacctc ttaaatgagg tagcaataaa tacataacat aaggagggga
     4681 aatcttgaat tgtaagtgta ggttttgtaa aaagaaatta aatacaaatg atgcttataa
     4741 agtagagcat attacatctg ggggaaataa gcagaataga tattattgca atgaacagga
     4801 atatagaaaa gagcaacagg atatatattt ctggaaacaa tgtcaacttg gaatagatta
     4861 tattatggga tatacagtta taaataatca aaaaaataag atgttacaag agataataaa
     4921 aggtggttat acgagagaag aattatatga ttgcatcatt gagaagaaag atgaagttat
     4981 agaattactt aactatagaa aagatataga agaagaatat cctaaattat gttatgtgtt
     5041 tacaatattg aaaggaagta ttagagatat aacaattaaa aacaagcaaa tgaaatatga
     5101 aaaagaaaat gaaaggatat acaaagaagt agagaaaagt gaagaatatt atgaaataat
     5161 tgctcctaaa aaagtaattt ataataaaag acaaagttta tttgacaaag tgaaaggagt
     5221 gtatcaagac gagtagttat gaaaaaataa tagaggatag gggtattata gaaacattgg
     5281 ttttaggcac aatgttaaaa tcattaactc tttttagtga atataaaatt agcgaaagtg
     5341 actttataat agataaggta aaattctttt tctcgttagg aagaataatg tctaaaacac
     5401 ataacgagct tgatgaaata agtgtagcta aatttgtatc ttcaaataaa ctcaaatctg
     5461 aatatgaaag atatggtggt tggaatagct tatcatctgc tatggagtat ggaaaagaaa
     5521 ctaatatagc agcatatatt gatgatttag ctaagaataa tttactaatt gctttagata
     5581 aaagaggttt taatgtaata aaagaaatgg agcataatgg gttaaaattt attccatttg
     5641 aaatatttca atctatgaaa tgtagagaag tggaagaatt ttatgagggg ttaatatctt
     5701 catgtagtgt aaattctata aaaaataata tgaaagttga aaatctttta cttactaaag
     5761 atgatagaga aaaattaaag caaaaaactg aagcaggaac accttataat attatatttg
     5821 aatacactga aaaagaaata ggattgagta ataatgaaga atctaagtat atatatagct
     5881 taccaatatt atcaaatagg actaatggga ttggtaaggg tggtggtata aatataatag
     5941 cagggttttc aggcatagga aaaactactt tactattttt taattacata ttagctatga
     6001 tatatagagg agagaagata gtcatatttg cgaatgaaca aaaatctcaa tattttaaga
     6061 gtatgttagt atcgtttata gcatataaca tatttaatta tcatgattta gatagaaata
     6121 aaatagataa tggagatttt acagactttg aagaagactt aatggaaaaa atagaaaaat
     6181 ttttaagaga tagatgtttt gcagagaatc taaaatttat ctatatggaa gaatttgaaa
     6241 tatctgaaat actaaggaaa agtaaagaac ttgttacaca tcagggattt actggaatag
     6301 cagtggatac atttaaatca gaagattcgt cagatgcaca ttatacagga aaattaattg
     6361 aaaattctaa attactcgat agttttggga acaaatataa tgtaataact atgttatcta
     6421 tgcaattact tacagctcaa gaaaataaaa gctcatatct ttcggcagga gatttgtcag
     6481 aagcgaaagc tgtcaaaaat gtatgtggtt tattaaaact tatgagaaaa gtagtaaatg
     6541 agttagaatt agattctact aacaagaaat tctttttaaa accatataaa ttaaaatata
     6601 ataaattaaa aaagactact gaaagagaat atatccagtt tgatgcaaaa gatttgcaaa
     6661 aagaatatag attattattt ttaaataagt caagaagagg tcaagatgga gatgtaattt
     6721 tactaagatt ttatggtaag actggtagat tagaagagat aggaaggtgt gagaaggtct
     6781 acagaggaca attgtcatac taggtgatga ctatggaaat aaaggatttg actaatgaac
     6841 aggtgataaa ttttatggaa tatttaggtt cggacttatc tcctaaaagc aatgatagac
     6901 agttaatatt caatacttgt ttatgtcata atggtgattc atacaaatta ttttattata
     6961 cagaaagcaa aacgtttcat tgctatagtt cttgtggtca tataggtagc ttaatagatt
     7021 tattaataca cataaataaa tatgagttta aagatgctat taatgaaata aaagatttct
     7081 ttggtatttc aaatcaacca atgctaagaa aaggatttag aataaagaaa aaagtagaac
     7141 agatacatga tattaaagat atacaaatag aattacttcc tacccctaaa aagccatatg
     7201 tatataaaac ttttcagcaa gtcccaattg aagagtggga aaatgaaaac atatcttttg
     7261 aagtccttaa aacatatcaa gtatattata atctatatga aaatcaaata gtaattccac
     7321 atttctgttg gcatgataga gctaggcctg ttggtattag agttagaaac ttagatgaag
     7381 ataaggctaa aagttttggc aaatatattc cattatggta cgacaatagg tgctataatc
     7441 atagattaag tcttaatcta tatggattaa atgtaaataa aaaatctatc aaaaattcca
     7501 aaaaagttat tgtatttgaa ggtgaaaaat cggtgttaca aatggctact atgtataaaa
     7561 ataatccctc tgtagctata tgtgggagta attttagtag agagcaaaaa aagatattaa
     7621 tagatttagg tattgaagaa ttaatcatag cttttgatag acaatttaaa gttaaagatg
     7681 atgaagaata tgttatatgg agagataaga tatataaatt agttcaagat attaaagatg
     7741 tagtgaatat aagtgtaata tgggataaat ataatttatt gggctacaaa gatagtccta
     7801 gcgataaagg aaagacaata tttgaaaaac tattaaaaaa cagaattaat atagagaact
     7861 ttgttaaaca ataaaaaagt aaagaaatta attttatgag aaaattttta ttgtttaggg
     7921 atttattagc agatgctaaa taaaatatgt gttttaatat gagctagaat aacaagtaga
     7981 ggagagataa gatgaaaata gtgagcataa gtatagattt actattaaag aatatgtgta
     8041 tttgtattct tgataatgga cacattcgag agttatccaa tagagatata atagctgata
     8101 aatttatctt tgaaatttat aaaattttta ataatcataa ttgcgataaa ctagtgttag
     8161 atatatcagg aattggaatt gcccattatc aaaatttaga ggaacatata gataaaaata
     8221 aattaattgg tgtttattta gcagaaaaga ataattctaa taaaatacta atgaatttcc
     8281 ttaataatga gtcattttat aatctaggac tagagatttg tgtaaaaaat aatggaggta
     8341 ggatatatcc agatatgtct aaatacgatg aaatagaaag ggggattatt gagtgtatta
     8401 tgttagcaaa ttattatagt aacccatcaa tagaaagtga gtaatatatt ttaatatgaa
     8461 ttggaggtag gttgcatgat aaatttttca ataaaggacg tacatatggg agatagatta
     8521 tattgtatga ataatatagg aaattataaa aaaggtgaaa tttatacagt tagtgatact
     8581 agtttttttc aactcttcca taagattaaa ggagaaaatt gcttaccaat tgattgttat
     8641 tttatatgtt taccaataaa agaaaaagta gaaactaatc ctaaatcaat gactaaagaa
     8701 gaaatagaag atgaattagg atataaaatt aaaataacgt ctaaatagta aaatgaggtg
     8761 atattatgtt gaatatcttc aataaaaaat atggtagaaa tgtaaaaata acagaggcag
     8821 aacctccttc tcctcctccc aaacaaccaa acttcgatat ggataatata caatatgata
     8881 aagaaatgaa aagaaagcta ttagaaatgg aaaaaataaa aatagactca gaaatgaaaa
     8941 aaaatagtga agaatggatt tggattgaag gatataaggg tacagataga gatatgaaat
     9001 gtagaaactt tcagtatgaa ctaaataaaa cttatagtgt tgaagaaata catagaggaa
     9061 atataaatat atatgactat ggattccatt tgtgtttaga tttaaaagat gttttcagat
     9121 gttataattt agatttaagt aatagatttt tcaaagtaaa agccttagtt agaagagagg
     9181 ataaatatag gtatgggatg aaacaatctt tttcatcaat acgtccagga gcaaaatgta
     9241 gcataggtgc taaagaaata atacttttta gagaattaac ttttgaagaa ttaaaacaag
     9301 atatccaaga caagtttcct aaaataaaca atgaactgga atggaatctt atcaaaaaat
     9361 taggtattta taaatattac aataaaatat ttataaataa aatgaaagaa attggttatt
     9421 cagatatttt ttcacaggtt ttatttgatg aatatcatcc gttaataaat ccacatagaa
     9481 ctatgaatat acttgacaag gcaattgcat attcaaaaga aaatttatct aaagatatga
     9541 taatatattt gcttatgaaa gaaggctgta gatgatagta tttgaattac taataataat
     9601 atttattgct atattaatgg taactatatt tctttcatgt ttgcttgttt caattagtat
     9661 gattcctaaa ataatagacg aaataaaaga cattattgat aaaattaaaa aggagaaaaa
     9721 ataatatgga gtttaaaaag gaattagaag ttatggaaga ttcagaaatt aaatttatac
     9781 aaagtttaaa tagaataaat aataatatcc gaataaatga agaaacaaaa atatgtttag
     9841 aaaaagaaat ttatgcttct tctagggttc taagtatgct ttgtgattta aatgaaatat
     9901 attctaaaca gaataaataa cacattaaaa agttagtttt aataggaggt ggtaattatt
     9961 aaatatgtaa tgagtgactt acatggtatg tatgataaat ttatttcaat gttagagcaa
    10021 atcaatttca attcaaatga ccatttgtat attttaggag atgtattaga tagaggggat
    10081 aagtctttag agattataga ttatataaga aagtataaga atataactct tctaaaaggt
    10141 aatcatgagt taatgtttca agaaagttat acagatgtaa atagtagttt tctttggttt
    10201 tataatggag gtaaagatac attttatgat ttaggaatga agtcttatga atataaagaa
    10261 aatttttata aatatattaa gaaccttcca tatttagaga taatagataa ttttatacta
    10321 gtccatgctg gactatacct tccaaataac tatgagaatc tcagtataga gcaaataatt
    10381 gagttacaag aagaagatat ttgtgtgtgg gatagaacaa tattaaatac taataaacat
    10441 ataaaaggat acacaattat tattggtcat acaccaactc aaaacattaa agattatgaa
    10501 aaggctgaaa tatataaaca ggaaggaatt ataaatatag attgtggagc atgttttgat
    10561 aatggtaaat tggcttgttt aagattggat gatatgaaag aattttatat ttaagaggtg
    10621 ttatatggac atattaaaaa tcagttacaa caatctaaat gctatatcaa aaatatcaga
    10681 agtattaagg gatattgtaa atgaggacac tttaatagta tgtataggta gtgatagagt
    10741 tttagctgac tcattagccc ctatgattgg cagtatacta gaaaaatcta ctattagaaa
    10801 taaaatattt ggagtattag gagattcaat acatgcttta aatttagaac aaaaaataca
    10861 agcaataaaa aataattatc caaattcaaa tataatagcc attgatgctt gtataagtaa
    10921 aatttcagat aaaggaacaa ttataattag caataaacct gttaaaccag gtttgggggt
    10981 agataagaag ttactagaag ttggtgatta ttcaatagtt gggattatag gtagaaataa
    11041 atatgatata tatgatactt cagaccctga acttatactt gatttagctg atgttatatc
    11101 gaaatcttta atatctatat tattagaaaa agaagaaagg atgattgtat gatacaggaa
    11161 gtcagacaag aaatactatt aaaaaatggt ttgattttat atacaggtga tttggtagaa
    11221 ataaaataca aatcagatga agatgtaata gaacatactt gtaaaggtaa aattaaagaa
    11281 gcaaaagaat tatttattaa attagataca agtaagaaat ataaaacttc tgaaagaatg
    11341 atttattctt gggaattaaa agaaataaaa aaggtggatg atgaagatga aaaagataat
    11401 taatagtaaa tatagagcat tatatttatt tatatttttt ctaattttca atttaatata
    11461 tagtttatat tttggaagga atacggagat tggattttat tctagtacat cttgcataga
    11521 agaatatata ctggatatta taacctctat aggactattt ttctcaatga tgttagctgg
    11581 gtttgatgtt acagaaaact ttataaacag tttaagggat tattgtgaaa aagatgcaaa
    11641 gtgaggtgaa taaattggaa ttaaaggatt atataagtaa agtagaatta aatgatatgc
    11701 cactaacttt agataatcag tacatgttaa atgaaataag catatctgaa tatggtaaaa
    11761 tatttgctgt aaaagacaca tcaggaagaa catcttatta tactgaaata gaatttaata
    11821 aactatttag aggaattaat agcatcctag agaatattat ctaatatata tttttaagga
    11881 actgagggtt gagagctatt aattaatagc tctctatttt tttgaaaggg gagagttaat
    11941 ggaaaaggaa ttatatagct ttagcaaatt aaatgcattt cacacatgtc catttcagta
    12001 ctatcttaca tatgtaaaaa atttaaatcg agaacaaaat tgttatggtt attatggtaa
    12061 tgaattacac aaattgctag aagaactaca gcaaagaaaa ataacaaatc aagaagctat
    12121 tcaaaaatat aatgaagtta tagaatatgc aaatctaatg gattacaact ttcctacacc
    12181 aaattcaaga attaattatt tagaatgtat cttacattat atagaaaatt ttgtaccaat
    12241 tgagtgtgat aaatactaca tagaagaata ctttgagttt gacattaatg gaatcacaat
    12301 gaggggatac atagatttat attatgtaat agatgataaa atttatgtta tagattacaa
    12361 atcgagtagt aaattttcca aaaaagacct acctaaaaaa tcaatgcagc ttatcttata
    12421 tgcaatgtat cttaaagaaa aatatccaga taaagtaatt gaatatgtag ctttcgatat
    12481 gtgcaagtat atgaagaatg aaaaaggggt gttaattgaa agaaataaga ttgataatat
    12541 aaacaattat gaaagagcta tagtaaaaat taaatatact aaagaactag aaaaacaatt
    12601 aattgatttt gttgttgata cagttactca aataaaacag ctagatttca acgatgagag
    12661 tgtttggtgc aaaaatgatg acaaaagtaa tcagtttttt tgtaaaactt tatgctctca
    12721 ttataacaaa ggagaagatt tatgttaata gatagaggaa attataaatt gtataaaggt
    12781 gattgtttag aagtaatgga tggattaata aaattaggag taaaattcga tgcaattata
    12841 acagacccac cttatgggac tactcaaaat aaatgggact cagtaatacc attagacaaa
    12901 atatggagta ggattaataa attaataaaa cctaatggtg caatagtttt atttggaagt
    12961 gaaccattta catcattact cataaggagc aatttgaagg attttaaata taattggatt
    13021 tggcagaaaa ataaaagtac tgggttcttg aactcaaaaa aacaaccttt gaatgaccat
    13081 gaaataatta gtgtttttta taaaaaacaa tgtgtatata atccacagat gacaaaagct
    13141 gaaaaaatat ataatagagg gtatgtagtt agaaataaag acgaagggat acaacaatct
    13201 gataattatg gggaacaaaa atcttttatg caagttgata caggtttaag atatccgaag
    13261 agaatacaat attttaacaa caattttaca aaacaacaac tacaccctac tcaaaaacca
    13321 atagatttac tggaatattt aataaaaaca tacacaaatg aaggtgattt agtattagat
    13381 tttacaatgg gttcaggttc aactggagtt gcctgtataa acactaatcg tagatttgtt
    13441 ggtatagagt tggatgaaaa atatttcaat ataagtagta aaaggttgtc agaaatatct
    13501 taatttaacc ttttaaacaa attgatattt tgctatagca taattatctt gaaataacga
    13561 gatggtcata tgactaaatc ataataaaat gtggatttta ataagattgg agagtgattt
    13621 ttatagagat aaataaagtc tataatattg attgtattga atatatgaaa acattaccaa
    13681 aagaatgtgt cgacttaata atagcagacc caccatatta tagaatatta aaagaaactt
    13741 gggataatca atgggggaca gaaaatgact atttcaaatg gtgttgtaaa tggataaaag
    13801 aaagtaaaat aatattgaaa caaaatggct caatgtatat atggaactgg tttgataata
    13861 tatgtgaaat aggtcatatt tgtagagaaa atgattttta tatcagaaat cttatttgtt
    13921 ggaatagggg aggtggaaga gaaagaaaca attggggaag taaaaaagaa gacttgttgt
    13981 acatatcaaa aacaaaaaat ccaaccttta atcttaatga tgtcctccta agtgcggatg
    14041 atataaacag aaaaatgagt aaacaagcat gggaaagagg aaagtatgag agaaaaggca
    14101 gaaaaaatta caatcaagaa agtgtgaatc catctaatgt ttggtatgat tcaatcttaa
    14161 catataattc aaaagaaaag gtgaaccatc cctctcaaaa accattaagt ttatgtaata
    14221 gaataataaa agcatcaagt aatgaaaatg atttagtata tgttccattt gcagggagtg
    14281 gttctgagat aatatcatgt attaaaaata atagaaacta tattgcaaca gaaataaata
    14341 atgactatat agaagaaatt attattccaa gaataaagaa tttaaaggag agtgtttaaa
    14401 tgaatatacc aaaacaagta aaagtaggag gtttatccta taaaatagaa gagacagaag
    14461 aacctattct agtaaataat caattatgtt atggattagc tgattatgga actgaaacaa
    14521 taaagttatc taataatcta caatcacaaa gagtaaaaga agctactttc ttacatgagt
    14581 tattccattg tatatttaga gatagatgca tagagagtga agatgaggaa tatttagtgg
    14641 atgtattagc taaagggctt catcaagtaa ttatagataa tccttatatg tttgaaacaa
    14701 atgaaaaaaa agaaaatgaa aggattttcg accccgcagg atataatcca aaatttggtt
    14761 aagtgggagg aatataatga ataaaatatt tgtattttta ggatattctg gctcaggtaa
    14821 agatagtata gtaacagaaa tttcaaagca attcaatatt ccaattctaa tatcacatac
    14881 aacaagacct cctcgtggcc aatcagaaat catcaataaa acatatcatt ttgtagataa
    14941 taaattcttt aagaaagaaa aaaataattt tattgaaatg agaaaatata tagttcatga
    15001 tggcagcact tggctatatg gtatacataa atctgaatta gaaaataaaa aatatgcttt
    15061 gactatagta gattcatgtg gatataaggc gttagaaaaa tattttatgg gaacaaaaac
    15121 taagttaata ccatttttta taaatacaga tgagaatata ttaagaaaaa gacttattaa
    15181 taggggtgat aatgctaaag aaatagagag aagattagca gatgataaac ttaaatttaa
    15241 tgattttcta aataatgaaa gttatattgc aataccaaac aacactaatc taattaatgc
    15301 agtggagcaa gtcaaaatac atatgaaaga agggattgaa tggtattagc cctagatata
    15361 tccatgtcct caactggatg ggcagttata aatagaaata aaaaggtatt aaaatatgga
    15421 aaaatagtaa ctaaaaaaga taaattcaag tctgaagatg agagaatgtg ctatatctgt
    15481 aatactattc aagagttaat tataactcat aatattcaaa tagtcttagt tgaagaccaa
    15541 ttcacttcaa gaaattctaa aacaattctt agtttaagaa aactcttagg ggcaataatg
    15601 aggactgtaa aattaaataa tatagagatt gaatatatgt atccagtttc tattagaaaa
    15661 tatctcatga ataatggtaa agctaaaaag gaagaagtag ctacatatgt aagagaaaac
    15721 attattgata ttggagaata tattgatagg acttgtaagg ctaaaacaag cgatatgtat
    15781 gatgctattt ctattggaat agcttatttg aataaacttg atactaacag ataattttaa
    15841 aaagggagat taaattaatg agtataaatg ttaaaataaa gaaattaaac caagacgcaa
    15901 tagtaccatc ttatgcaaaa gaaggcgata gtgggataga tttatataca ctaaaagata
    15961 cagtgattcc agctaaatca gcagtagcaa tacctacagg tatagcatta gaaattccat
    16021 atggatatga aatgcaagtt agacctagaa gtggtataag cttaaaagga atgtttttta
    16081 caagatatgt gactatatat gataaagtgc aacctaaaaa acaaattaga aaaatttttg
    16141 tagcacatcc aactgtaaga ttaggtacag tggattcagg atatagaggt gaaattaaaa
    16201 taattacata taatgaagaa gactttgaaa ttctaattcc caaacatact aaattagctc
    16261 aaggagtatt tcaacaagtt ccaaaagcta tattaaaagt tgtagatgaa ttaaccagct
    16321 ccgaaagagg aagtaatgga tttggaagca caggtatata gatgaataag tatagaatac
    16381 tagaaaagta taattattat tatgatgaca aatgtatgac attatatcat ttagaaaata
    16441 tctatacagg attaagattt tctattgaaa aaattgaatt agagtactat aatatatcct
    16501 agactaactt tgggaggagt attaaatgta cagatatgat gattatgtaa atttaataaa
    16561 tgacttagga ttgccaaaag agattaggaa taaatgtgtt agagttataa tgacactaga
    16621 gaacatgcaa gatattatgg ttgtacataa gtttaagact tatgttgtta atgaaaaata
    16681 cattgaaaaa gtagcataaa aatgattaag tcatctatta atttaggtgg cttttttgca
    16741 tttatgtcga atatttcatc tacttcatat tgtattagat gctataatta aaatatatgt
    16801 cgaaaggtag atggtgagga tgtttaagga gatatttgaa aaggtattta tgtcagaaga
    16861 ggttagtaaa actgtaaaag aagttaataa tattattaag gatactaaga tacaagtaaa
    16921 aggtggattt ggagaactta gattagattt tatacttagc agattaggca aagattacat
    16981 aataattaaa gacattattg taccaggctc aaataagact acacaaattg atagtattgt
    17041 gatttctatg tatggaatat ttgttataga atgtaaaaat tttagtggat atatttacgg
    17101 aaatgataaa gataaagtat ggactcaaat tgtagggaaa actaaaaata cattctataa
    17161 tccggcaaga caaaattatg ctcatattaa agcaatagaa aatataatag gagataaata
    17221 caatatatat tcaataattg ttttttcaga caaagctaca ctaaaaaatg taatgataag
    17281 tggaaataat attatagtta taaatgaaag tgagatatta agtacaataa gtaaatgcaa
    17341 agatgtgact attgataaag aagaaataaa aattattaga gataaaattt ttgattgtat
    17401 gaaaaccact aatcaaaata ctagagagca tgttagaaat ttaaaaaata ttactgaaga
    17461 ggataagtgc cctagatgtc aaattggaca tttgattaaa agaaaaggtc agtatggtga
    17521 attttgggga tgtagtcaat atccaaaatg taaatacact agaaaataaa aaaacaacct
    17581 cttaaattcg attttaaggc tttgtaaaaa ttaatgctta taattaatcc tattcaggaa
    17641 tttgattgct taaaacgcaa aatataaaaa ataataaatt actagtgtaa ggcatagaat
    17701 tgtgtattct atgtcttatt tctttataat taagtaattt caataattat ataaataaaa
    17761 gaattaaaaa agtcagttga aagtcagcta aaatgactat tttttgcctc ctctgtatgg
    17821 tataataagt atatgaattt tcataggagg gataaccatg aacgctttta taagaaaaag
    17881 aaataaaaat tatgtagtgt acttagaatt tagagatgat gaatcaggaa aaagaaaaca
    17941 aaaaaatatg ggagcatttg ataaaaaaag agatgctaat aaaagattgg ctgaagttaa
    18001 ggacagtata tataaagata gttttcttgt cccaaatgaa atcactctag ctggatttct
    18061 attagacttt cttgaaaaat ataaagataa tatttcggca tctacatata aaagctatat
    18121 tgctatttgt aaaaatcata ttaatccttc tattggaaaa tatcgtcttc aagagttgag
    18181 aaatattcat atacagaatt atatagatga tttagctggt aacttaaacc ctcaaactat
    18241 taaagtacat ataaatgtat tgagacttgc aataaagaga gcgtatagaa ttaagttaat
    18301 aaaagaaaat attatagatg gaatagagag tccaagaatt aaaaaattta aaaatgaaat
    18361 ttatgataaa gagcatatgt taaaattatt agaagtagct aaaggaacta atcttgagct
    18421 tcctattagt ttggctatag gtctaggatt gagactttca gaagttttgg gattaacttg
    18481 ggataatatt gattttgatg aaaatacaat aacagtaaat aagataacta gtagattaga
    18541 tggttctgtt atacttaaag agccaaagac agaaagctct gttagaaaaa tatttgcacc
    18601 aatagagctt atgaatttac taaaaaatta taggctggag cagaataaga agttattgag
    18661 aagtattgtt agaaatgaat ataacttact attttttgat agaaaaggga atccaattgc
    18721 tgaagatgta atgagtaaga aatttagaaa attcttagag aataatgact tgcctcatat
    18781 tagatttcat gacttaagac attcgcacgt tactttactt ataaattcta aagtacctat
    18841 aaaagttata tctgaaaggg taggacattc aaatattaat actactctta atgtatattc
    18901 tcatgtactt aaagaaatgg acaaagaagc ttctgataga atatctgaaa acttatttaa
    18961 ggctaattaa taattattat caataaatac aaaattaata ataaaagcaa aggtataaac
    19021 ataaagggga atttttacaa agccttagaa tggcatatag agcgtcagat tttagaagat
    19081 attaaaattt tatatttcca atatttatga tataataaac atagcaagga ataaatattt
    19141 gaaaaaagct gtgagtggtg tttccataga aatttcctca ctttcttatg aaaggaggtg
    19201 aaaactatgg aaaatctaat gatgagtatt atagctggtg ttatagctag ttatatctac
    19261 gataaattaa aatgccactc tgagcgacca actaagagtg gctgggaact taatattaag
    19321 ttccataaaa acaaacatta ataactaatt aatggaaaca tcactcaaag ttaagtaaaa
    19381 tatgtttcct tgcttttatt ataccacaaa ttaagaatat taaaacaaaa ttttgtaaat
    19441 acatacttaa ttatcaattt aatcatttgg aaaaaattgg atttgattgt atgaaattat
    19501 tgctgtaata tttaaattaa ataaacaaat gatgggagag ataattatgt atttaagaaa
    19561 attatcaaag tcagaattaa ttgtaatgaa atttatatgg aatttagata caaaagtaaa
    19621 atcatatcaa atcattaatt atatgaaaga aaaatatagt tggtcagaaa aaacaacatt
    19681 aaaaacttta tctaagttat caaataaaag gttcatatat gttcaagaaa caagtcaatg
    19741 tacatactat acaatttcaa ttaaagaaga taagtatcat gaatttatat cacaaaaaat
    19801 acataagctc ttagggtgca attctataaa aagcctttta gcatcattgt ttcaagaaga
    19861 attaacagat gaaaaaataa cttcattgga agaatgggta aaaaactggg aagaagagga
    19921 ataaagataa tatatactat tccttaaaaa tctatttaaa gaataattga aattttgatt
    19981 ttaatataat ttctttaatc aactctaaca tacaaaaata agactctttt caatattaac
    20041 taaagagcct tattttataa ttgattatat tttagttaac ttttgtgtat caaaactata
    20101 ctcatatact tctaatgttt cagaatttac aattagagtt cctactgttt taatgctatc
    20161 ttctttatca gtataagtat cagaaccatc agtgctagat tcatcttgtt taacattttt
    20221 aacactgatt atttcaaact catagtgtgg aaaatctata ttttcagctt ttttatatct
    20281 tgagttacca taattatatg tataacaata ttcatgtgct tttgaacctt ttgctttata
    20341 tatagcatct aaagcttttg agatgtcact tcttttagta ttaccagtta acatataatc
    20401 tggagttaag cattggtcta aaatttcatt ttttatatca cctattatta ttgggaaatt
    20461 agcactagta actaatgatt tgtcattagt ttcatttact aaaaatacag gatattcttt
    20521 gtgtattgta gaagataata gtgcatctat taaattatca gaacttacaa tacatatttg
    20581 atttggatgt tttaattcat ctttataaaa atgcttaatt attttttcat tagtgtcaag
    20641 tctgtcaaca ccaccaattc tagttgcttt tgtactgttc actaaagagt cgtttattga
    20701 tgctgttcct ccaatagcat atgtttttac cccatctgtt ttaaatggta tattctttcc
    20761 atttgttaat acaactggat ttttatattt agcagcaact ggagatatac ttatagaatc
    20821 agcctctcct tggtaagcat ttgtaagata tatttcatca acctttttta gattgcttat
    20881 ttcttttgca acattataac ttgtgtctat tctgtctttt ccttctatcc taataacctt
    20941 tatttttttg ttttttagta tattttctac atttttgctt atagaattaa ctccacctat
    21001 taaatagatt tttttaaatt tatctaatct tttaagagta gaatttggta tagaattttg
    21061 tttagtaagt aatattggcg agttagaagc tccagctaaa ccactagcac tcaacccatc
    21121 tgcaagactt ttatctgtgt ttataattat tgcttgggtg tattttcttc tgtctgctat
    21181 taaacctgct gtttcatact tatttgctcc ttgtattcta tgagctggag gtctaaattt
    21241 tagttcttta gctgaaacta ataatgtgct agacatgaca atagaagata ttatagctaa
    21301 ggttaatgct tttcttttga atttcatatt gtaaaatcct cccatccttt tatactatga
    21361 atgatagtgt atattatttt agttgtcaac tctctatgta tattttatta tatatataag
    21421 aataaattgt attaattata tcgttacgaa aactttttgt atataataaa agttcacttc
    21481 tttatttatt cttataacaa tcctctaaag aactaattaa gctttctata gaattagaat
    21541 gtacttcttc taaaaatctc tttgtttcaa atattttata ttcttctttt gtaataagta
    21601 ttgtataatg tgtaattctt tttatcctct cagaatctaa aaaacgttta atagtgagtc
    21661 ttgaaagaag agttagtgta gttgtcgcct tccagttata ttctaattcc atagcttcca
    21721 taacttcttt tgatgttact atatcattct tattccatat gaatttcata acttttaatt
    21781 cagcttgtgg tattttttgc atggatatgt acattccttt ctgcaattaa aatatcaata
    21841 tatatagaca ttaaaataat aaaatatatg ttataagtat atattaacat aaagtaaaag
    21901 aagatgtctc aatcttaaga catcttcttt attaaatttt tattcttcaa attttcgata
    21961 ttcaattatt tcttttatca atctaattat cgctttttgt tcttttactg ttaaactatc
    22021 aattaactca taagcttcag acgaaagagt ttcaacatca ttttcatttt gtataatatt
    22081 ttcaaataaa acttctaaag gtatctctaa tgcatttgca attttaaaaa ttgaacgaag
    22141 tgatgcttta ctttcacctc tttcaatttg accaatataa gcaggaaaca aaccagcctt
    22201 ttttgctaat gtattttgat tataccctgc ttttcttcta taatttctta ttcttttacc
    22261 aattacatta tatatttcat cttcttctaa catttttatc attccttatt agattatatt
    22321 agtttatt
//
LOCUS       DAMJYR010000054        22135 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.054, whole genome shotgun sequence.
ACCESSION   DAMJYR010000054 DAMJYR010000000
VERSION     DAMJYR010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 22135)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22135)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22135
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.054"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            92..433
                     /locus_tag="PC471_002067"
     CDS             92..433
                     /locus_tag="PC471_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family transcriptional regulator"
                     /protein_id="HDF2789195.1"
                     /translation="MKEENFINVEYEDKRFSINSKEVLYIEKQEYEVKIYTESGNYEV
                     GENAPCIRKLLKEENFFKCSNSYIVNLDKCEFYLSNNCIFVNNNFIRILGSERKKKLK
                     KKLEKLYDYED"
     gene            584..997
                     /locus_tag="PC471_002068"
     CDS             584..997
                     /locus_tag="PC471_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1653 domain-containing protein"
                     /protein_id="HDF2789196.1"
                     /translation="MLELKNNLGISERKLKYPCIYKHFKGKYYATMGLSKAIDDIENI
                     CEIYGKENLIQNRNKYKLVIRHTEREEDIYVYRDLDGNFYHKKEEDTNDLVLYKTLYD
                     DTGIFARPLDMFLEKVDTDKYVNSIQEYRFEEVYK"
     gene            1199..1840
                     /locus_tag="PC471_002069"
     CDS             1199..1840
                     /locus_tag="PC471_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HDF2789197.1"
                     /translation="MGNTFYIVRHGQTDWNILGKTQGHGNSDLTPQGIEQAKELSEDI
                     GKYSIDYIFSSDLGRAMQTAQILGDKLNIEVQKTEALREMGFGVWEGLLIKEIQKDYS
                     DIYATWRNEPHLVNIPEGETLKIIKERVDAFIKELNEKYDNKNIILVTHSITLRVMLL
                     SFLESGMENIYRIKQDNTALNIVEFKDYGPVIVKMNDTSHIKNHVKINNSALE"
     gene            1864..3117
                     /locus_tag="PC471_002070"
     CDS             1864..3117
                     /locus_tag="PC471_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HDF2789198.1"
                     /translation="MSKVIVVGGGASGMMAALSASKNNNEVILVERNGELGRKLRATG
                     GGRCNFTNNREIEDFFDKVVSNKKFLYSSFYTFTNKDLISYFESRNLEYKIEEENDHK
                     VYTKNDKSIEVIEVLNKDLLNHNVKIMYNKKVIDIITEEIALKDDSNKDKSKYLIKGI
                     ILDNGDKILGDKVIISTGGVSYSKTGSDGSMYKILKKHGHTLNKLYPALVPLTIEEKW
                     IKDLQGISMKNVEISCKIKKRKISKSGDMLFAHFGITGPCVLIMSSYINKIIEKEKVE
                     LNIDFLPNLSTDEISSIIRAFPNKNVLNNLKQILPQNFLKEIFSLLSLVDKKASDLSK
                     ADEIRIIEYIKNMKLTCNGTTGINTGMVTSGGISVKEINSSTMESKLVKNLFFTGEVI
                     DIDAETGGYNLQIAFSTGYLAGISV"
     gene            3163..3813
                     /locus_tag="PC471_002071"
     CDS             3163..3813
                     /locus_tag="PC471_002071"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HDF2789199.1"
                     /translation="MGNLVIAVDGPAGAGKSTIAKIVAKKLNINYIDTGAMYRAVTYK
                     CLKSGIDVNNEKEVIQIAENSDIDFKDNNIYLDKEVINEEIRTIEVSNNVSNVAKIKE
                     VRQLMVEVQRKIGMKNSVILDGRDIGSYVFPDADYKFFLVATPEERGNRRYKELCNKG
                     YNTTLEEVIEDIIRRDEIDSNREFAPLVKANDALEIDTTGKTIEEVVEEVVSKINL"
     gene            3864..4460
                     /locus_tag="PC471_002072"
     CDS             3864..4460
                     /locus_tag="PC471_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HDF2789200.1"
                     /translation="MNFYRFVINIFKGFSKIFFKYEVIGAENIPDRGNIVIASNHKSN
                     LDPIFLAAAIENREIAAIAKKELFKVKPLGFILKKLHVMPINREKPDVSTIKTILRSV
                     RDGYVLGIFPEGTRIKGDSFGKAKAGLSVFTIKSKSKVVPVSIISKYKLFSKVIVYIG
                     EPISFEEHFKEKLSNDDHERISQEILEVIKQNYFKYSK"
     gene            4473..5312
                     /locus_tag="PC471_002073"
     CDS             4473..5312
                     /locus_tag="PC471_002073"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HDF2789201.1"
                     /translation="MNIKIAKNAGFCFGVKRAMKMAWDEVEKNDSGIYALGPLIHNKQ
                     AVAKYEEKGLKTVNEIDTIPNHENMIIRSHGVPENIYKEAKDKKLKIVDTTCPFVKKI
                     HTVVSEYHNKGYEIIVIGDMKHPEVIGINGWCENSAIIIKTLEQMENMEFDNSKRYCL
                     VAQTTINPELYISIVNKLSDKLEEIVFNDTICSATKTRQESAKELAKEVDCMIVIGGK
                     HSSNTQKLVKVCEDLVPTFAIETKDELDVNTLKKYKNLGITAGASTPNWIIEEVVTFL
                     ENL"
     gene            5557..6711
                     /locus_tag="PC471_002074"
     CDS             5557..6711
                     /locus_tag="PC471_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenic efflux protein"
                     /protein_id="HDF2789202.1"
                     /translation="MEMLISASEEAFLHVGSMIGFFILLFGYINYKTSGNFTNIISKN
                     RKFQPLIGALIGAIPGCGGSLAIMPLYINGKLSFGAIIASLIASMGDAAFVLISSNIK
                     MYFFVTIVSTITGIITGQLVDYFKLEEKLGLKNRRKSDKYDISKNNPKENKEQHNHDE
                     IILDTLAKSHGNTNRLAFIITHGKGYKIYIGIIVIGFIFMSLAHSGLNLPIIEKLHSL
                     EEVIAVIGILFSIIYMWCFKKVFKNSNQKEEENKKISLREMLIHSVGEISFVITWIFI
                     AYLIYDLIILILGGDEYLVKLVLSTGVISVFIGAGLGLIPGCGIQIVLMSFYLKGTIP
                     LGAVIANSISQDGDALFPLLAMDKKSSLWAMIITTIPAILVGMIVYAFLG"
     gene            7017..8714
                     /gene="ade"
                     /locus_tag="PC471_002075"
     CDS             7017..8714
                     /gene="ade"
                     /locus_tag="PC471_002075"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HDF2789203.1"
                     /translation="MGNLKRQCDVSSGREKADIVLKNGTIINVFTEELITGDVAIVGD
                     TIVGIGDYKGNVEIDCSNKYISPGFIDAHMHIESTMVMPIELSKKLLKSGTTTIIADP
                     HELVNVKGVSAIDFLLESTKDIPLNVYIMVPSSVPATSFETNGVGKFSAKDMESYVNN
                     PRILGLGEVMCFNDVINSENEILDKLELFKNKVVDGHAPNINGKSLQTYVCAGIENDH
                     ECITFDEVYEKLRAGLKILIREGSAAKNLKSIVSGMLKHNLPIEEFMFCTDDKHLDDI
                     EKQGHIRWNIKCAIDLGMEPVRAIKVATYNSAKAYGLRKIGAIGAGYKADIVVLNDLD
                     KMEVDSVYKDGNLVNEEMFSNYNYEIKDKELLNTVKFKYINKEKIQLKVSEKNYVMEI
                     VPYQILTNKVYESLPCADGYFVPNKEYSKLCVVERHRMTGNVAIAPLKGFGIKNGAIA
                     TTVAHDSHNIIVAGDNDDDILVAINYLKEIQGGYVIVSNGKVLAHLSLQVAGLISTFT
                     AEEVQEITDNMLEIARKMEVPEYVDPFITLSFMALPVVPQIRLTDLGLFDVEEFKFI"
     gene            9066..9530
                     /gene="bcp"
                     /locus_tag="PC471_002076"
     CDS             9066..9530
                     /gene="bcp"
                     /locus_tag="PC471_002076"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002586316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HDF2789204.1"
                     /translation="MLSIGTKAPEFTLEDKDGNKVSMSDFKGKKVVVYFYPKDNTPGC
                     TRQACAFRNAYDGFKKEDIQVIGISKDSIKSHQKFAEKHELPFILLSDPDLVAIKAFD
                     VWKEKKMYGKTALGVVRATYIIDENGIIEKVFEKAKPDTNAQEILEYLEKQE"
     gene            9540..10283
                     /gene="yaaA"
                     /locus_tag="PC471_002077"
     CDS             9540..10283
                     /gene="yaaA"
                     /locus_tag="PC471_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HDF2789205.1"
                     /translation="MIIMLSPAKNMKNIEVFDRDLSLPCFIDNTKEIVENIKTFGIED
                     FKNKMKINEKLAVLNKNRFESIKFDRLGNPAILTYDGIQYKNIEAENFTRKDEEFANS
                     CIRIISGLYGVVKPYDSIYEYRLEMQTKLQVGEFKNLYEYWGNRIYKELIKEKTAIVN
                     LSSNEYSKSIEKFIKDSDTYITCTFKVNKNGILKVESTQAKKARGMMTKYIVKNRIRD
                     IEELKKFDLEGYKYKENLSNNSEYIFVKE"
     gene            10411..13068
                     /locus_tag="PC471_002078"
     CDS             10411..13068
                     /locus_tag="PC471_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HDF2789206.1"
                     /translation="MENHKQMPWHSRATHEILESLDSSEEGLTDTEAEKRLKENGLNE
                     LRKKPPRTTLQMLWSQITDAMVMILIGAAILSLIFGEFTEAFVILIIVTVNAVIGIVQ
                     EKKAESSLEALKNMNSPTARVMRDGEENVIPASNLVVGDIVFLEDGAMVPADLRLIET
                     SSLKIQEASLTGESVPSEKDADTILPKECVLGDRSNMAYTSSIVTYGRGVGVVVATGM
                     DTEVGNIAHLLDSQDDFDTPIKRKLNTVGKTLSVIGIIVCVVIFAIGAFYQRPLLPMF
                     MIAISLAISIIPEGLPATATIVMALGVQRMAKRNALIRKLPAVETLGGATVICCDKTG
                     TLTLNKMTVTHIAVNGDFESGKVTSIDTASEKHPLVYKEIVYAGALCNDACINPDKTD
                     EILGDPTEGALIFMAKKFGIDQEALEEEYPRVFEQPFDSDRKCMTTVHNIDEKIIAYT
                     KGAVDEILDLCTKILTSKGERNITEVDKKNIHELCLNMSKDALRVLGFAKREISSIPK
                     EDSENIEYDLTFIGMVGMIDPPRTEVIDAVSTCKEAGIRTIMITGDHKVTATTIAHEL
                     GIWSEENTVISGDELDNLSDDELDEAVKNTTVFARVSPFDKLRIIQSLKRIGEVPAMT
                     GDGVNDSPALKSADIGIAMGISGTDVAKDSSDMILMDDSFTTIAYAIKEGRRVYRNIQ
                     KVIQFLLVGNIAEILTLFVAILLNWDTPLLAVHILWVNLATATLPALALGVDPPSRNI
                     MKHQPVKSTTLFEKNLIQRVVTQGIFVAIMTILAYKIGLNIENHSVGQTMAFCVLAFS
                     QMLRAFSQRSNTDSMFNRNNGKNPFLLISFLTSAFLIAVILFVPVFRNAFNLTVLNNL
                     EWLVTIVLAIMSVIQVEVGKIIKRKKLKK"
     regulatory      13122..13224
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            13334..14599
                     /locus_tag="PC471_002079"
     CDS             13334..14599
                     /locus_tag="PC471_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase"
                     /protein_id="HDF2789207.1"
                     /translation="MYNKETICVQGNYKPGNGEPRVLPLYQSTTFKYSSIDQLAELFD
                     LKVDGHIYSRISNPTIQAFEEKISLLEGGVSSVAVSSGQSANMLAVLNICKSGDSILC
                     SSKVYGGTFNLLGPSLKKFGIDLISFDLDSSEDEIVELAKENTKVVFAETLANPTLEV
                     IDFEKIANVAKRINVPFIVDNSLASPVLCNPLKYGANIVTHSTTKYLDGHASSVGGII
                     VDGGNFNWDNGKFPELVEPDPTYHGISYTQKFGNAAYATKARVQLLRDYGNCLSPFNA
                     YLTNLNVETLHLRMERHSENALKIARFLEKHENVDWINYPGLEDNKYYENAKKYLSRG
                     CSGVLSFGVRGGLENAKKFVEKLQIASLVTHVSDVRTCVIHPASTTHRQLTEEQLIAS
                     GVLPSLIRLSVGIENVEDLIADLNQALNF"
     gene            14629..15519
                     /gene="metA"
                     /locus_tag="PC471_002080"
     CDS             14629..15519
                     /gene="metA"
                     /locus_tag="PC471_002080"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HDF2789208.1"
                     /translation="MALILPKGLPVINKLLDEGIDVIYKEDFKKELKYEENIDTKIAI
                     LNLMPIKIDTELDLLRRIDKTGFNVSVEFIKISTRESKRSCNEYVKEFYKTFDEAKGE
                     YFDGFIITGAPVEQMEFEEVDYWNELEEIMDYSKRKTKSTLYICWAAQASLYKYYNVK
                     KLPLSQKCFGVFKHKVDKDSKIVDGFENEFFAPHSRHTTVNIEALKENKELSIVSHSK
                     EAGPYIITNSRDVFVMGHSEYDKYTLDKEYKRDINKGDKISIPQNYYINDDPSEEPTV
                     KWKKHSELLFRNWIKNYLIQ"
     gene            16010..16453
                     /locus_tag="PC471_002081"
     CDS             16010..16453
                     /locus_tag="PC471_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDF2789209.1"
                     /translation="MDEMSFKKELLELTRDINMKFTTLLSDFYQPLGITAVQALILSE
                     LCEHGEKKISDLGKNLNMTNSNVSVICQRLEKNGFLNRIRDIEDQRIVKVKVTNKSLD
                     IQEHISSSIFDSYFENMTSEKLQDMEDIIEGLEKLNKLLTYIDCK"
     gene            16484..18301
                     /gene="ftsH"
                     /locus_tag="PC471_002082"
     CDS             16484..18301
                     /gene="ftsH"
                     /locus_tag="PC471_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HDF2789210.1"
                     /translation="MDEHKPQKRSALYYYTVIIIVVMFLNALIFPSLMDKKMVEIDYG
                     KFLTMLDTGKVKSVEIENNRIAINPSSSKDKNIYITGRMDDPELVDRLKNAKVEFTKV
                     IPKENSPLLSILLTWIIPIGILMLFGNLMMKSMQKRMGGNAMQFGKSNAKMYVSAQSG
                     KRFNDVAGQDEAKEALTEIVDFLHNPEKYKKIGAQMPKGALLVGPPGTGKTLLAKAVA
                     GEANVPFFSISGSEFVEMFVGMGASRIRDLFKQAKEKAPCIVFIDEIDTIGKKRDNGS
                     GLGGNDEREQTLNQLLSEMDGFDGGIGVVILAATNRPDSLDKALLRPGRFDRRIPVEL
                     PDLGGREAILKVHAQNVNVSSDIDYNAIARSTSGASGAELANIVNEAALLAVKSGRDI
                     VEQQDFDESVETVIAGYQRKGAVISDKEKKIIAYHEIGHALVAAMQKHSAPVHKITII
                     PRTSGALGYTMQVAEDESVLMSKEEAIDKITTFTGGRAAEEVIFNTCTSGASNDIEQA
                     TKIARAMVTRLGMSEEFDMVALETVSNQYLGGDTSLACSSDTAAKIDEEVFKIVKACH
                     KNAIEILENNIDKLHELANYLLEKETITGDEFMEILNLN"
     gene            18479..21181
                     /locus_tag="PC471_002083"
     CDS             18479..21181
                     /locus_tag="PC471_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase KdpD"
                     /protein_id="HDF2789211.1"
                     /translation="MDRPNPDILLEKIKNEEEKLSRGQLKIFFGYAAGVGKTYSMLES
                     AQNLKKVGVDVVVGYIEPHTRPETLALLDGLEILPVKEIEYKSIKLKEFDLDAALARK
                     PEVILVDEFAHSNVAGLRHTKRWQDIEELLLAGINVYTTVNVQHLESLNDIVEIITNV
                     SVKETIPDKFLDTNTQLELIDVEPDVLLERFNEGKIYKKEQATRAKNNFFIKDNLVAL
                     REIALRKTAERVNKEVQMTRLSKGDVTVIPTSDTLLACISPSPSSAKVIRTASRISDS
                     TFAKWIALYVETPNTARLPFDEQKQLQNNLKLAKKLGGEIIVLHGENIIEQILRIAKL
                     RNVTKIVIGRNHSSNKRFSKRFKKDIVDKLIDEVDYIDIHIIPYKFASDVKYKPKKDK
                     SSIKSKFKISKVDFIKLIFITLMISILAYAVQSMGFIRENILLIYMLGVVLVSMWTKG
                     YSTGIISSVFNIVLLNYFFTAPLYTLSIADSNYIVTLVVFSIVGIITSTLTSKIQHEA
                     ETAAKREENTKMIYQIIKGFLRLSNKDDIVNKGIELLSLSLSRDVICYLNVDKHNKSK
                     LYKKNTNNKDKNDLDSEDEKAVASWVLSNSTVAGNDTDTLPGSKGYYIPIVGMNLTLG
                     VVGISCIDSKLDTEDISLIETIIAQMAIALDREILSEAKENTNLEIERERLRSNLLRA
                     VSHDLRTPLAGISGAVSTIIKNKGTIGQDIIDELLSGVYEDTQWLIRLVENLLSMTKI
                     DEGKLEVKKHSELVEEIISEALQKIKKRIENALIDINIPEQILFVPMDAKLIEQVLIN
                     LIDNSLKYSKEDCKIEINVYEKDDYVWFEVSDNGPGISKELKKHIFDRFFTGEEGAKD
                     SRKGVGLGLSICKSIIQAHKGEIMVENNKDKGSTFKFSLPKENE"
     gene            21215..21913
                     /locus_tag="PC471_002084"
     CDS             21215..21913
                     /locus_tag="PC471_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2789212.1"
                     /translation="MSKKLILLVEDDKTIRKFISTALLTQDYDVKEAITGKEGISIAV
                     SYSPDVVLLDLGLEDMDGIEVIKAIRQFSNIPIIVVSAREQDRDKVEVFDAGADDYLT
                     KPFSIVELLARVRVAFRHSQVEAQQKDDVKSTFEVDKLLIDFDKRKVIVDDVEVHLTP
                     IEYNILSLLAKYHGKVLTHNFIIKEIWGSVIGNETKSLRVFMATLRRKIEKQPANPRY
                     IITEVGVGYRLNDE"
BASE COUNT         8846 a         2366 c         3984 g         6939 t
ORIGIN      
        1 ttaagtaaat cagtcttaca tatatttccg atagattata ttgataaaaa acactaaaat
       61 taaattaata aaagtacttg gagggatgga aatgaaagag gaaaatttta ttaatgttga
      121 atacgaggat aaacgttttt caattaatag taaagaagtt ttgtacatag aaaagcaaga
      181 gtatgaagta aagatttata ctgaaagtgg taattatgaa gttggagaaa atgctccttg
      241 tataagaaaa ctattgaaag aagaaaattt tttcaaatgc agtaatagtt atattgtaaa
      301 tctagacaag tgtgaatttt atctcagcaa taattgtatt tttgtgaata ataattttat
      361 aagaatttta ggttcagaaa gaaaaaagaa attaaaaaag aaattagaaa aattatatga
      421 ttatgaagat taatattggg aggctgtatt ttatacagcc tctttattta ttgattgtat
      481 tattaataaa ttttaaatta tgataaaata taaaacaagt aacttatttc agaataaaat
      541 actttttaaa agaaagggac gagatatggg agattataat aaaatgcttg aattaaagaa
      601 taacttaggt atcagtgaaa gaaagttaaa atatccttgt atctataaac atttcaaagg
      661 gaaatattat gctacaatgg gtcttagcaa ggctattgat gatatagaaa acatatgtga
      721 aatatatggt aaagaaaatt taatccaaaa cagaaataaa tataaattag ttattagaca
      781 tactgaaaga gaagaagata tctatgttta tagggattta gatggaaact tttatcataa
      841 aaaagaagaa gatactaatg atttagtttt atacaagacc ttatatgatg atacaggaat
      901 atttgcaaga cctttggata tgtttttaga aaaagtagat acagataagt atgtcaattc
      961 aattcaagaa tacagatttg aagaagtata taaataatat aagagaaata tgtttataaa
     1021 tttagaaaaa ttctttaaca gtacaataat ttgcagaaat acaaatgaga tttattgtaa
     1081 ataatatgtt agattagatt ataaaataga aacaaagtaa agtattatat gatataatta
     1141 agttttgaga agtgataatt tatatttaat aaaaaatagg catatattag gaggacatat
     1201 gggaaatact ttttatatag taagacatgg acaaacggat tggaatatat tgggaaaaac
     1261 acaaggtcat ggtaattctg accttacgcc tcaaggtata gaacaggcaa aagaactttc
     1321 tgaagatatt ggcaagtatt caattgatta tatttttagt agcgaccttg ggagagcaat
     1381 gcaaacagct caaattttag gagataagct caatattgaa gttcaaaaaa ctgaggcact
     1441 tagagaaatg ggattcggag tttgggaagg ccttttaata aaagaaattc aaaaagatta
     1501 ttcagatata tatgcaacat ggagaaatga accacactta gtaaatatac cagaaggcga
     1561 aactcttaag attataaagg aaagagtaga tgcttttata aaggaactaa atgaaaaata
     1621 tgataataaa aatataattc ttgttacaca ttcaattaca ttgagggtta tgttgttgtc
     1681 atttttagaa tcaggtatgg aaaatatata tagaattaag caagataata ctgctttaaa
     1741 tatagtagaa tttaaagatt atgggcctgt tatagtaaaa atgaatgata caagtcatat
     1801 aaaaaatcat gtaaagataa ataattctgc actggagtag gaatcaatta taaggagaaa
     1861 tttatgagta aagttatagt agttggtgga ggagcttctg gaatgatggc agctttgtca
     1921 gcttcaaaaa acaataatga agtaatatta gttgagagaa atggagaatt aggaagaaaa
     1981 cttagggcaa ctggtggcgg tagatgcaat tttaccaata atagagagat agaagatttt
     2041 tttgataaag ttgtcagtaa taaaaaattt ctctatagta gtttttatac atttacaaat
     2101 aaagacctta tttcgtattt tgaaagtaga aatttggaat ataagattga agaagaaaat
     2161 gaccacaaag tatacactaa aaatgataaa tctatagaag taatagaagt attaaataaa
     2221 gatttactca atcacaatgt aaagataatg tataataaga aagttataga tattataact
     2281 gaagaaattg ctctaaaaga tgattctaat aaggacaagt caaagtatct aataaaaggg
     2341 attattttag ataatggaga taaaatacta ggagacaagg ttattatatc aactggagga
     2401 gtgagttatt caaagacggg ttcagatggt tcaatgtata agattcttaa aaagcatgga
     2461 catacattaa acaaactata tcctgcttta gttccactta caatagagga aaaatggatt
     2521 aaagatttac aaggaatatc catgaagaat gtggaaatat cttgtaagat taagaaaagg
     2581 aaaatatcaa aatctggaga catgctattt gcccattttg ggataacagg accttgtgtt
     2641 cttataatgt cttcttatat taacaagatt attgaaaagg aaaaggtaga attaaatata
     2701 gatttcttac caaatttaag tactgatgaa atctctagta taatcagagc atttccaaat
     2761 aaaaatgttc taaataatct taagcaaata ctacctcaaa atttcttaaa agagattttt
     2821 tctctattat ctttagttga taaaaaggca agtgatttgt ctaaggcaga tgaaattaga
     2881 attattgagt atataaaaaa tatgaagcta acttgtaatg ggactacagg tataaacact
     2941 ggtatggtta cttctggtgg tatttcagtt aaagaaatta attcttctac tatggaatct
     3001 aaattagtta agaacttatt ttttacaggt gaagtaattg atatagatgc agaaactggt
     3061 ggatataatt tacaaatagc attttcaact ggatatcttg cgggtataag tgtataaata
     3121 taatttttta ataattaaat atgaataaat agaggtgtat atatgggtaa tttagttata
     3181 gctgtagatg gtccagcagg agctggaaaa agtacaatag ccaagatagt agcaaaaaag
     3241 ctaaatataa attatataga tacaggtgct atgtacagag cagttactta taagtgttta
     3301 aaaagtggta ttgatgtcaa taatgaaaaa gaagttattc aaatagctga aaattcggat
     3361 atagatttta aagataacaa tatatatttg gacaaagaag ttataaatga agaaataaga
     3421 acaatagaag taagcaataa tgtttctaat gtagcaaaaa taaaagaagt aagacagttg
     3481 atggttgaag ttcaacgaaa aataggaatg aaaaattcag taatactaga tggtagagat
     3541 ataggttcat atgttttccc agatgcagat tacaaattct ttttagtagc tactccagaa
     3601 gaaagaggaa atagacgcta taaagaactt tgtaataaag gatataatac tactttagaa
     3661 gaagtaattg aggatataat aagacgagat gaaattgatt caaatagaga atttgcacca
     3721 ttggtaaaag ctaatgatgc actagaaata gatactacag gaaaaactat agaagaagtt
     3781 gtagaagaag ttgtatctaa aataaatctt tagtttttta tgcatcataa tgaaaataaa
     3841 tgtagtgttg aagggagata aacgtgaatt tttatagatt tgtaataaat atttttaagg
     3901 gtttttctaa aatatttttt aaatatgaag ttataggtgc agagaatatt ccagatagag
     3961 gaaatatagt aatagcatca aatcataaat cgaatcttga ccctatattc ttggctgctg
     4021 ccatagaaaa tagagaaata gctgcaattg caaagaaaga attgtttaaa gtaaaaccat
     4081 tgggatttat attaaaaaaa ttgcatgtta tgcctataaa tagagaaaaa ccagatgtat
     4141 ctacaataaa aactatttta aggtcagtta gagacggtta tgtattagga atatttccag
     4201 agggtactag aataaaagga gattcttttg gtaaggctaa ggcaggtctt tctgtattta
     4261 caataaaaag taaatctaaa gttgttccag tatcaataat atctaaatat aaacttttta
     4321 gtaaagtaat tgtatatatt ggagaaccaa tatcatttga agaacatttt aaggaaaaat
     4381 tgagtaatga tgaccatgaa aggatatcac aagagatttt agaagtgata aaacaaaatt
     4441 actttaaata ttcaaaataa gggggtaaag ttatgaacat aaaaatagca aaaaatgctg
     4501 gcttttgttt tggagtaaaa agagcaatga aaatggcatg ggatgaagtt gaaaaaaatg
     4561 attctggcat atatgcttta ggtcctctta ttcataacaa gcaagcagta gcaaaatatg
     4621 aagaaaaagg tcttaagact gtgaatgaaa ttgatacaat accaaatcat gaaaatatga
     4681 taataaggtc tcatggagtt cctgaaaata tatataagga agccaaagat aaaaaattaa
     4741 aaattgtgga tactacatgt ccatttgtaa agaaaataca tacagtagta agtgaatatc
     4801 ataataaagg ctatgaaata atagttattg gagatatgaa gcatccagaa gttataggta
     4861 taaatggatg gtgtgaaaat tcagctataa taataaagac cttagaacaa atggaaaata
     4921 tggaatttga taactccaaa agatattgtc ttgttgcaca gactacaata aatccagagt
     4981 tgtatataag tatagttaat aaactatcag ataaattaga agaaatagta tttaatgata
     5041 caatttgttc agctacaaaa acaagacaag aatcagctaa agagttagct aaagaagttg
     5101 attgtatgat agttatagga ggtaaacaca gttcaaatac tcaaaagtta gtaaaggttt
     5161 gtgaagactt agtaccaact tttgcaatag agactaaaga tgaattggat gtaaatacgc
     5221 ttaaaaaata taaaaatttg ggtattacag caggggcatc tacacctaat tggataatag
     5281 aagaagttgt aacattcctt gaaaatttat aatttgatag gtaataaaaa taggtggaca
     5341 ttgttataat ttatcataat atccaccttt gatttcatat tacatgtggt aaatagataa
     5401 cctaatatca aaaagtgtgt atcttaagat gaaaaactaa gagtataaga tgcgcacttt
     5461 ttaatattag aaattcaaaa gaaatattga tattgattat caatagaaaa tagaatataa
     5521 tataattgtt gcaaaattag tctaggaggt aaattaatgg aaatgttgat atccgcttct
     5581 gaagaggcgt ttttacatgt aggttctatg attggatttt ttattttgtt atttggatat
     5641 attaattata aaactagtgg aaattttact aatatcatat caaaaaacag aaaatttcaa
     5701 cccttaatag gtgctttaat tggagctata ccaggttgtg gcggaagttt ggcaataatg
     5761 ccattgtata taaatggaaa attaagcttt ggagcaataa ttgcaagttt aatagctagt
     5821 atgggagatg cagcatttgt acttatttca tcaaatatta aaatgtattt ttttgtcact
     5881 atagttagta caattacagg aataataact ggacaattag tggattactt taaactagaa
     5941 gaaaaattag ggttaaaaaa ccgtagaaaa tcagataaat atgatataag taaaaataac
     6001 cctaaagaaa ataaagaaca acataatcat gatgaaataa ttttagacac tctagctaaa
     6061 tctcatggaa atacaaatag acttgcattt ataataacac atggaaaggg atataaaatt
     6121 tatataggaa ttatagtaat cggttttata tttatgtcac ttgctcactc tggacttaat
     6181 cttcctataa ttgagaagtt acattcattg gaagaagtaa tagcagtgat tggaatatta
     6241 ttttcaataa tttatatgtg gtgttttaaa aaggtattta aaaattctaa tcaaaaagag
     6301 gaagaaaata aaaagatttc tttgcgagaa atgcttatcc attctgttgg agaaatatca
     6361 tttgtaatta catggatttt tattgcatat cttatttacg atttgataat attaatttta
     6421 ggtggagatg aatacttagt taaattagta ttatcaacag gtgtaataag tgtatttata
     6481 ggtgcaggtc ttggacttat tccaggttgt gggatacaaa ttgtgcttat gtcattttat
     6541 ttgaagggaa ctataccact tggtgcagtg atagccaact ctatttctca agatggagat
     6601 gcacttttcc cacttttagc aatggataaa aaatcttctt tatgggctat gattattact
     6661 actataccag caatacttgt aggtatgata gtttatgcat ttttaggtta aatataatta
     6721 tgaataaaga agtattaaaa aaataataaa gcacataaat ttatttataa gaatagtgaa
     6781 gattaaatat aaaaataaaa aattcaatat gaattaaatt attgcttaaa tattaatgag
     6841 aacatgggag ataatattat tcctcatgtt ctctctattt aattaaagga attatggaac
     6901 aaatttcaag tgatactttc tatcaaaact catatataat atatattaat aaattaaact
     6961 ataatacgta atacagtcag tatattgata taaaaaactt aggaggagtt gtaaaaatgg
     7021 gtaatttaaa aagacagtgt gatgtttcaa gtggaagaga aaaagctgac attgtactaa
     7081 aaaatgggac aatcataaat gtatttacag aagagttaat aacaggagat gtagcaatag
     7141 taggagacac tattgttgga attggcgatt acaagggcaa tgtagaaatt gattgtagta
     7201 acaaatatat atcacctggc tttattgatg cacatatgca tattgaatct acaatggtaa
     7261 tgccaattga attatcgaaa aaattattaa aatcaggtac gacaactatc atagccgacc
     7321 cacatgagtt ggtcaatgta aaaggagtta gtgcaataga ttttttatta gaatcgacaa
     7381 aagatatacc acttaatgta tatattatgg tgccttcatc cgtacctgca acaagttttg
     7441 aaactaatgg agttggtaaa tttagtgcaa aagacatgga gagttatgta aataatccta
     7501 gaattttagg tttaggagag gtaatgtgtt ttaatgatgt tataaattct gaaaatgaaa
     7561 tcttggataa actagagctt tttaaaaata aagttgtaga tggacatgca cctaatataa
     7621 atggtaaatc acttcagaca tatgtatgtg caggaataga aaatgaccat gaatgtataa
     7681 cttttgatga agtatatgaa aaattgagag caggattaaa aattttaata agagaaggaa
     7741 gcgcagcaaa aaatttaaag tctatagtaa gtggtatgct aaagcataac ttgccaattg
     7801 aagaatttat gttttgcaca gatgataagc acttagatga tatagaaaaa caaggtcaca
     7861 taagatggaa tataaaatgt gcgattgatt taggtatgga acctgttagg gctataaaag
     7921 ttgcaactta taattctgca aaagcatatg ggttaaggaa aattggagca ataggtgcgg
     7981 gttataaggc agatattgtt gtattgaatg atttagacaa aatggaagtg gatagtgtat
     8041 ataaagatgg taatttggta aacgaagaga tgttttctaa ttacaattat gagattaaag
     8101 ataaagaatt gcttaataca gtcaagttta aatatattaa taaagaaaaa atacagttaa
     8161 aagtaagtga aaaaaattat gtaatggaga ttgtacctta tcaaattttg acaaataagg
     8221 tatatgaatc tttaccatgt gcagatggat attttgttcc aaataaagag tattcaaaac
     8281 tttgtgttgt tgagagacat agaatgactg gaaatgtagc aattgctcca ctaaaaggat
     8341 ttggcataaa aaatggagca attgctacta ctgtggccca tgattctcat aatattatag
     8401 ttgcagggga taatgatgat gatattctag tagctataaa ttacttaaag gaaatacaag
     8461 gtggttatgt aattgtatca aatggaaaag ttcttgcaca cctaagtctt caagtagctg
     8521 gattaattag tacttttaca gctgaagaag tacaagaaat tactgataat atgttggaga
     8581 tagcaagaaa aatggaagtt ccagagtatg tagacccttt tattacactt tcttttatgg
     8641 cacttccagt tgttccacaa ataagactta cagatttagg tctatttgat gttgaggaat
     8701 ttaaatttat atagatagta taaaagtaat aataaatgag gctttctcaa aatagagata
     8761 agtctctttt tattgttgaa atgatgaaaa tatatttgat ttcatacaaa aaagagcatc
     8821 tcatgaacta aaaattttat ttttagatat ccttatttta attaatctat catttaacat
     8881 tgttttaagc atagatatct taataaaact attttcagtc tattttaaca tataaataca
     8941 tgaaaattat atatttatga aactttatac ataaaaaata aaatatttta tttatatata
     9001 gatttatagg gtaataatat aagcatagta taaaattatt aataaaaaca aaaggagaga
     9061 tatttatgtt gagtatagga acaaaagcac cagaatttac actagaagat aaagatggaa
     9121 ataaggtaag tatgtcagat tttaaaggca aaaaagtagt agtatatttt tatcctaaag
     9181 ataatacacc aggatgtaca cgtcaagctt gtgcttttag aaatgcatat gatggattta
     9241 aaaaggaaga tatacaagtt attgggataa gtaaagatag tataaaatca catcaaaaat
     9301 ttgcagaaaa gcatgaactt ccttttattt tactttctga ccctgactta gttgctataa
     9361 aagcttttga tgtatggaaa gagaaaaaaa tgtatggaaa aactgcattg ggagttgtac
     9421 gtgcgacata tattatagat gaaaatggaa ttattgaaaa agtctttgaa aaagcaaaac
     9481 cagacacaaa tgcacaagag attcttgaat atttggaaaa acaggagtaa taagaattta
     9541 tgattataat gttatcacca gctaaaaata tgaagaatat agaagtattt gatagagatt
     9601 tgagtttgcc ttgctttata gataacacaa aagaaatagt ggaaaatata aagacatttg
     9661 gtatagaaga ttttaaaaat aaaatgaaaa ttaatgaaaa attagctgta ttaaataaaa
     9721 atagatttga aagcattaaa tttgatagat taggaaaccc tgcaatctta acttatgatg
     9781 gaatacaata taaaaatata gaagcagaaa attttacaag gaaagatgaa gagtttgcaa
     9841 atagctgtat tcgaatcata agtggattgt atggtgttgt aaaaccatat gatagtatat
     9901 atgaatatag attagaaatg cagacaaaat tacaagtagg tgagtttaag aatttatacg
     9961 aatattgggg aaatcgtata tataaagaac taatcaaaga aaaaacagct atcgttaatc
    10021 tttcatcaaa tgaatacagc aaatccatag aaaaatttat taaagatagt gatacatata
    10081 ttacatgtac ttttaaagta aataaaaatg gcatattgaa agtggaatct acacaagcaa
    10141 agaaagctag aggtatgatg acaaaataca ttgtaaaaaa taggataaga gatattgaag
    10201 aactgaaaaa gtttgattta gaaggttata agtataaaga aaacttatct aacaatagtg
    10261 aatacatatt tgtaaaagag tagaagtttt gttaaatatt aaatggcatt gttaaattta
    10321 ggtaaaatat acttagtgtg tattgaattg aacttgattt taaaatataa ttattcttaa
    10381 attaaaaaaa gaaaggagtg gtcttccaaa atggaaaatc acaaacagat gccttggcat
    10441 tcaagagcaa cgcatgaaat tttagaaagt ctagattcat cagaggaggg gctcaccgat
    10501 accgaggcgg aaaaacggct taaagagaat ggcttaaatg aacttagaaa aaaaccacct
    10561 agaacaactt tacaaatgct ttggagtcaa attacagatg ctatggttat gatactaata
    10621 ggagcagcaa tattatcttt gatatttggc gaatttacag aagcttttgt aatactaata
    10681 attgtaacag taaatgcagt tattggaatt gttcaagaga aaaaagcaga atcatcatta
    10741 gaagcattaa aaaatatgaa ttcacctaca gctcgtgtta tgcgtgatgg tgaagaaaat
    10801 gttattccag caagtaatct tgtagtagga gatattgttt ttttagaaga tggagctatg
    10861 gttccagcag accttcgttt aatagaaaca tcaagtttaa aaattcaaga ggcttcttta
    10921 acaggagaat ctgttccatc agaaaaagat gcagatacaa tcttacctaa agaatgtgta
    10981 ttaggagacc gtagcaatat ggcgtataca tcttcaatag tcacatatgg tagaggtgta
    11041 ggtgttgtag tagcaactgg tatggataca gaagtgggta acatagcaca tcttttagat
    11101 tcacaagatg attttgatac tcctataaaa cgtaaactta atactgttgg aaagacatta
    11161 agtgtcatag gtattattgt atgcgttgtt atatttgcaa taggtgcatt ttatcagaga
    11221 cctctcttgc caatgtttat gattgcaatt tcccttgcca tatcaattat tccagaggga
    11281 ttacctgcaa cagctactat tgtaatggct ttaggcgtac agcgtatggc taaaagaaat
    11341 gctttaatta gaaaattacc tgctgttgaa acattaggag gagctacagt aatatgttgt
    11401 gataaaactg gtacattaac cttaaataaa atgactgtta cacatattgc tgtcaatgga
    11461 gattttgaat ctggaaaagt gacatcaata gatactgcat ctgaaaaaca tccattagtg
    11521 tataaagaga ttgtttatgc tggtgcactt tgtaatgatg catgtattaa tccagataaa
    11581 acagatgaaa tattaggtga cccaacagag ggcgcattaa tttttatggc caaaaaattt
    11641 ggaatagatc aagaagctct tgaagaagaa tatccacgtg tatttgaaca gccttttgat
    11701 tcagatagaa aatgtatgac aactgttcat aatatagatg aaaagattat tgcttatact
    11761 aaaggagcag tagacgaaat tcttgaccta tgtactaaaa tattaacttc aaaaggtgaa
    11821 agaaacatta ctgaagtgga taaaaagaat atacacgaat tatgtcttaa catgtctaaa
    11881 gatgcattgc gtgtacttgg ttttgctaag cgtgagattt catctatacc aaaagaagat
    11941 agtgaaaata tagaatatga tttaacattt ataggtatgg ttggtatgat tgacccacca
    12001 agaactgagg taattgatgc agtaagtact tgtaaagaag caggtataag aaccataatg
    12061 ataacaggtg accataaagt tactgccaca acaatcgctc atgagttagg aatatggagt
    12121 gaagaaaata ctgtgatatc tggtgatgaa cttgataatt tgagtgatga tgaactagat
    12181 gaagctgtta aaaatactac agtatttgct cgtgtatccc catttgataa acttagaatt
    12241 atacaatctc taaaaagaat tggagaagta ccagcaatga ctggggatgg tgtaaatgac
    12301 tctcctgcct taaagtctgc tgatattggt atagccatgg gtataagtgg tacagatgtt
    12361 gcaaaagatt cttctgacat gattttaatg gacgatagtt ttacaacaat tgcttatgca
    12421 ataaaagaag gtagacgtgt atatagaaac atacaaaaag ttatacaatt tttgcttgtt
    12481 ggtaatatag cagagatact tacgttgttt gtagcaatat tattaaattg ggatactccg
    12541 cttcttgctg ttcatattct atgggtaaac cttgcaactg ctacgcttcc agcccttgct
    12601 cttggagttg acccaccaag cagaaatata atgaagcacc agccagtgaa atcaaccact
    12661 ttgtttgaaa aaaatcttat acaaagagtt gttacccaag gtatatttgt ggcaataatg
    12721 actattttag catataaaat tggtcttaat atagaaaatc attctgtagg tcaaacaatg
    12781 gcattttgtg tattagcatt ttcacagatg ttacgagcat ttagtcagcg ttcaaataca
    12841 gattcaatgt ttaatagaaa taatggtaaa aatccatttc ttttaatctc atttttaact
    12901 tctgcatttt taatagcagt aatactattt gtacctgttt ttagaaatgc ttttaactta
    12961 acagttttaa ataacttaga gtggcttgta acgattgttt tagccataat gtctgtaatt
    13021 caagtagagg ttgggaaaat tattaaaaga aaaaaattaa aaaaatagtt gacaaaatta
    13081 ggaaaaatat ataagatata aaacatagaa aaattaaata aatcttatcc agagaggtgg
    13141 agggactggc cctatgaaac ccggcaacca gtatatttat atacattggt gctaaatcct
    13201 gcggcgaaag ctgagagatg agtataatta agcttataca gagcctgagt tatatactca
    13261 ggctttattt attttaactc tcatctggta aatagtttaa ttttttttac taaaatctaa
    13321 gggggataca agaatgtata ataaagaaac aatatgtgtg caaggaaatt ataaaccagg
    13381 taatggagaa ccaagagtac tacctttata tcaaagtaca acttttaaat atagcagtat
    13441 agaccaactt gctgaattat ttgatttaaa agttgatgga catatatatt caagaataag
    13501 caatcctact attcaagctt ttgaagaaaa aataagttta ctagagggtg gagtatcttc
    13561 tgtagctgta tcatcagggc aatctgcaaa tatgttggca gttttaaata tatgtaaatc
    13621 aggagatagt atactttgtt cttcaaaagt atatggagga acattcaatt tactaggacc
    13681 tagtcttaaa aaatttggta tagatttaat atcgtttgac ttagattcaa gtgaagatga
    13741 gatagtagaa cttgcaaagg aaaatactaa ggttgtgttt gcagaaacac ttgcaaatcc
    13801 aactcttgaa gtcatagatt ttgaaaaaat agcaaatgta gctaagagaa ttaatgttcc
    13861 atttattgtt gataattcat tagcatctcc agtgctttgt aaccctttaa agtatggagc
    13921 aaatatagtt actcattcta ccacaaaata tttagatggg catgcttcaa gtgttggagg
    13981 aattatagtg gatggtggaa actttaactg ggataatgga aaatttccag aattagttga
    14041 gccagaccca acatatcatg gtataagcta tactcaaaaa tttggaaatg ccgcatatgc
    14101 aactaaagca agagttcagt tgcttagaga ctatggaaat tgtttaagcc cattcaatgc
    14161 gtatcttact aatttaaatg ttgaaacact acatcttaga atggagagac atagtgaaaa
    14221 tgcacttaaa atagctagat ttttagaaaa acatgaaaat gtagattgga ttaattaccc
    14281 aggacttgaa gataacaagt attatgagaa tgccaaaaag tatttatcaa gaggatgtag
    14341 tggtgtttta tcatttggag taagaggtgg gttagaaaat gccaaaaaat ttgtggaaaa
    14401 attacagata gcatctttgg ttacacatgt ttcagatgta agaacttgtg ttatacatcc
    14461 agcttcaact actcatagac aattaacaga agaacaatta attgcatctg gagtattgcc
    14521 ttcactaata agattatctg ttggaataga aaatgtagag gatttaatag ctgatttaaa
    14581 tcaagcttta aatttctaat attctaagag aaagagaagt gaaaagatat ggctttaata
    14641 ctaccaaagg gattacctgt tattaataaa ttattagatg aaggaataga tgtaatttat
    14701 aaagaagatt ttaaaaagga attaaagtat gaagaaaaca tagacactaa gatagctata
    14761 ctaaatctaa tgcctataaa gatagataca gaattagatt tactaagaag gatagataaa
    14821 acaggattca acgtgtctgt ggaatttatt aaaatttcaa caagagaaag taaaagaagt
    14881 tgtaatgaat atgttaaaga attttataaa acatttgatg aagccaaagg agagtatttt
    14941 gatggcttta taataacagg agctccagtt gaacaaatgg agtttgaaga agttgattat
    15001 tggaatgaac tagaagaaat tatggattat tctaagagaa aaactaaatc tacattatat
    15061 atatgttggg cagctcaagc aagtctttac aaatactaca atgtaaaaaa acttccacta
    15121 agtcaaaaat gttttggagt atttaagcat aaggtagata aagatagtaa aatagtagat
    15181 ggatttgaaa atgaattttt tgctccacat tcaagacata ctactgtaaa tattgaagct
    15241 ttgaaagaga ataaagagtt gagcattgtg agtcattcta aagaagctgg tccctatata
    15301 attacaaatt caagagatgt gttcgtaatg ggtcatagcg agtatgacaa atacactctt
    15361 gataaagaat ataagaggga tataaataaa ggagataaaa tatctatacc tcaaaattac
    15421 tatattaatg atgacccttc agaagagcca actgtaaaat ggaaaaaaca ttcagaattg
    15481 ttgtttagaa attggataaa aaactatttg atacaataaa ttttaagagt atataaatgt
    15541 taactaaaaa aatatagaaa tttataggta taaagttaat cttaatgata ctttatacct
    15601 tattttttta aaatttgacc ttataatagt agttttatat tttaagaaaa tcactttagg
    15661 tgtatgtaga tagaggtggc tttaatttta tatatggtgc gataatggga gactaaacct
    15721 ttatagtatc aataaatttg tattttaact tttaaaagag attaaaaagg gagaaatcag
    15781 tgtaaaatat atatatagat tttttattaa attttattaa aaatatcctg tataaaatta
    15841 aatatcaata agataacaaa atttcaattg aagttacaat attgtaattg acaataaata
    15901 cttaatacta taatatttaa tacattaaac attatagtgt taagtattta tttacagtat
    15961 gcacttcagc ctaaatttga aataaagtat tttcgtaggg agcgtttgta tggatgagat
    16021 gagttttaag aaagagctac tagagttgac tagagatata aatatgaaat ttacaacttt
    16081 attaagtgat ttttatcagc cacttggtat tacagcagtg caagctttga ttttatctga
    16141 gttatgtgaa catggagaga aaaaaataag cgatttaggg aagaacttaa acatgacaaa
    16201 tagtaatgta tcagtaatct gtcaaaggct tgaaaaaaat ggctttttaa atcgcataag
    16261 agatatagaa gaccaacgaa ttgtaaaagt aaaagttaca aataaatcat tggatataca
    16321 agaacatatt tcaagtagta tttttgatag ttattttgaa aatatgacca gtgaaaaatt
    16381 gcaagatatg gaagatataa tagaaggttt agagaaatta aataaacttt taacttacat
    16441 tgattgcaaa taacataaaa ataatttaaa ggagaataaa attatggatg aacataaacc
    16501 ccaaaaaagg tcagcactct attattatac agttataata atagtagtaa tgtttttaaa
    16561 tgctttaata tttccaagtt taatggataa aaaaatggtt gagattgact atggaaagtt
    16621 tctcacaatg ttggatacag gaaaggttaa atctgttgag atagaaaata atagaatagc
    16681 aataaatcca tctagtagca aggataagaa tatatacata actggtagaa tggatgaccc
    16741 agaattggta gatagactaa aaaatgcaaa agtagaattt actaaggtta ttcctaagga
    16801 aaattcacct cttttaagta ttttacttac atggataata cctataggta ttcttatgct
    16861 atttggtaac ttaatgatga agtctatgca aaagcgaatg ggtggtaatg ctatgcaatt
    16921 tggaaaaagc aacgccaaaa tgtatgtatc tgctcaaagt ggaaaaagat ttaatgatgt
    16981 agctggtcaa gatgaggcaa aagaagcttt aactgaaata gtagatttct tacacaatcc
    17041 agaaaagtat aaaaagatag gagcacaaat gcctaaagga gcacttttag taggacctcc
    17101 aggtacagga aaaacactcc ttgcaaaagc tgtagcaggt gaagcaaatg taccattttt
    17161 ctcaatatct ggttctgagt ttgttgagat gtttgtagga atgggagctt ctaggattcg
    17221 tgatttattt aaacaagcaa aagaaaaagc tccatgtata gtgtttatag atgagattga
    17281 tactattggt aaaaagcgtg ataatggaag tggattaggt ggaaatgatg aacgtgaaca
    17341 aactttaaat cagttgttaa gtgagatgga tggttttgat ggtggaattg gagtagtaat
    17401 acttgctgct acaaatagac ctgacagtct tgacaaagca ttacttcgtc caggaagatt
    17461 tgatagacgt ataccagttg aacttccaga ccttggagga cgtgaagcta tattaaaggt
    17521 acatgctcaa aatgtaaatg taagctcaga tatagattat aatgcaattg caaggtctac
    17581 atcaggagca tcaggtgcag aacttgcaaa tattgtaaac gaagcagcac ttttagcagt
    17641 taagtctggc agagatatag tagaacaaca ggattttgat gaatctgttg aaactgtaat
    17701 tgctgggtat caacgtaaag gagctgtaat ttcagataag gaaaagaaaa ttatagcata
    17761 tcatgaaata ggtcatgcac ttgttgctgc tatgcaaaaa cattctgcac ctgtccataa
    17821 gattactata atacctagaa cttcaggagc attgggatat actatgcaag ttgctgaaga
    17881 tgagagtgta cttatgtcta aagaagaagc aattgataaa ataactactt ttacaggtgg
    17941 tcgtgctgct gaagaggtta tctttaacac ttgtacatca ggagcatcaa atgatataga
    18001 acaagctact aaaattgcaa gagctatggt tactcgcctt ggtatgagtg aagagtttga
    18061 tatggtcgct cttgaaactg taagcaatca atatttagga ggagatactt ctcttgcttg
    18121 ttcttccgac actgctgcta aaatagatga agaggtattt aaaatagtaa aggcttgtca
    18181 caaaaatgca attgaaatct tagaaaataa tattgataaa ttgcatgaac ttgctaatta
    18241 tttattagaa aaagaaacta ttacaggtga tgagtttatg gaaatactta acttaaacta
    18301 aaatgattat acacaagata aaaatgccta ttgttaggca tttttatttt acaactaaat
    18361 tttttaaatt tatatttaaa tctttacata atcttaatca ttaaaaatac atcttaacta
    18421 caattttata tcggaattga tattataata gtaatgaatg gtgtaaggag gttttttaat
    18481 ggatagacct aatcctgata ttctattaga aaaaattaaa aatgaagaag aaaaactaag
    18541 tagaggtcaa ttaaaaatat tctttggtta tgcagctgga gtaggaaaaa cctactctat
    18601 gttggaatct gcacaaaatt taaaaaaagt gggtgtagat gtagtagttg gatatattga
    18661 accacacact agaccagaaa cattggcttt acttgatggc ttggaaattc ttcctgtaaa
    18721 ggagatagaa tataaatcta taaaactaaa agaatttgat ttagatgctg ctttagcaag
    18781 aaaaccagaa gttatattag tagatgaatt tgcccattct aatgttgctg ggttaaggca
    18841 tacaaaaaga tggcaagata tagaagaatt gctgttagca ggcataaatg tctacactac
    18901 tgtaaatgta caacatttag aaagtctaaa tgacattgtt gagataatta caaacgtatc
    18961 agttaaagag actataccag ataagttttt ggatacaaat acacagttag aacttataga
    19021 tgttgaacct gatgtacttc ttgaaaggtt caatgaaggt aagatatata agaaagaaca
    19081 agctactaga gcaaaaaata atttttttat aaaagataac ctggttgcac ttagagaaat
    19141 tgcacttaga aaaacagcag agagagttaa taaagaagtt caaatgacca gactatcaaa
    19201 gggtgatgta actgtaatac ctacatcaga tacacttctt gcatgtataa gtccatctcc
    19261 atcttcagca aaagttatta gaactgcatc cagaatatca gacagtacat ttgcaaaatg
    19321 gatagcttta tatgttgaaa ctccaaacac agcaagactt ccatttgatg aacaaaaaca
    19381 actacaaaat aacctaaaat tagctaagaa attaggtgga gaaataattg ttttacatgg
    19441 tgaaaatata atagagcaaa tacttagaat tgcaaagctg agaaatgtaa caaaaattgt
    19501 aataggtaga aatcattcaa gtaataaaag atttagtaaa agatttaaaa aagatatagt
    19561 tgacaaatta attgatgaag ttgattatat agatatacat ataattccat ataaatttgc
    19621 ctcagatgtt aaatacaaac ctaaaaagga taaatcatca ataaaatcta aatttaaaat
    19681 tagcaaagtt gattttataa aacttatatt tattactttg atgataagta ttttagcata
    19741 tgcagtacaa agcatgggat ttataagaga aaatatattg ttaatatata tgttgggtgt
    19801 agttttagtt tctatgtgga ctaaagggta tagtacaggt ataataagtt ctgtatttaa
    19861 tatagttctt ttaaattatt tttttacagc accattatat acattatcta tagcagattc
    19921 aaactatata gtaacactgg ttgtattctc aatagtaggt ataataacta gcactcttac
    19981 gtctaagata cagcatgaag cagaaactgc tgctaaaaga gaagaaaata caaaaatgat
    20041 atatcaaata attaagggat ttttaagatt atcaaataaa gatgatatag taaacaaagg
    20101 tatagaactt ttgtctctta gcttaagtag agatgtaatt tgttatttaa atgtagataa
    20161 gcataataaa tcaaagcttt acaaaaaaaa tactaataat aaggataaaa atgatttgga
    20221 tagtgaagat gaaaaagctg ttgcaagttg ggttttatcc aattcaacag tagcaggaaa
    20281 tgacacggat acacttcctg gttcaaaggg atactatata ccaattgtag gaatgaattt
    20341 aactttagga gtagtgggaa tatcatgtat tgattcaaaa ttggatacag aagatataag
    20401 cttaattgaa acaataattg ctcaaatggc aatagcgctt gatagagaaa tcttgtcaga
    20461 agctaaagaa aataccaatc ttgagattga aagggaaaga ttaagaagta atcttttaag
    20521 agcagtttcg catgacctta gaactccact agcaggaata tctggtgcag taagtacaat
    20581 tataaaaaac aagggtacta taggtcaaga tataatagat gaattactta gtggtgtata
    20641 tgaagatact cagtggttaa taagacttgt tgaaaattta cttagtatga ccaagataga
    20701 tgaagggaaa ttggaagtta aaaaacattc tgaactggtt gaggaaatta tatcagaagc
    20761 gttacaaaaa ataaaaaaaa gaattgaaaa tgcacttata gatattaata taccagaaca
    20821 gatattattt gttcctatgg atgcaaaatt aatagaacaa gttttaatta atttaataga
    20881 taattctttg aaatattcaa aggaagattg taagatagag ataaatgtat atgagaaaga
    20941 tgattatgtt tggtttgaag tatctgataa tggtcctggt atatcaaagg aacttaaaaa
    21001 gcatatcttt gacagatttt ttactggaga agaaggagct aaagattcca gaaagggtgt
    21061 tggacttgga ttatcaattt gtaaatctat aattcaagct cataaaggtg aaattatggt
    21121 agaaaacaat aaagataagg gttcaacttt taagtttagt ttgcctaagg aaaatgaata
    21181 gttttaaatt ttaaaattag ttttggagga agaaatgagt aaaaaactta tattgttagt
    21241 agaggatgat aagacaataa gaaaatttat atctactgca cttttaacac aggattatga
    21301 tgtcaaggag gctataactg gaaaagaagg tatatctata gctgtctcat attctccaga
    21361 tgttgttctt ttagatttag gcttagaaga tatggatgga atagaggtaa taaaagctat
    21421 cagacaattt tcaaatatac caataattgt agtttcagct agagagcaag atagagacaa
    21481 ggtagaagta tttgatgcag gggcagatga ttacttaaca aaaccattct ccatagtaga
    21541 gttattagca agagtaagag tagcatttag acattcacaa gtagaagctc aacaaaaaga
    21601 tgatgttaaa tctacgtttg aagtagataa attattgatt gattttgata aaagaaaagt
    21661 tattgttgat gatgttgaag tccatttgac acctattgaa tataatattc tttcattact
    21721 tgcaaagtat catggaaaag tgcttactca taattttata ataaaagaaa tatggggtag
    21781 tgttataggg aatgagacta agtctttaag ggtctttatg gcaacgttaa gacgaaaaat
    21841 agaaaaacaa cctgcaaacc caagatatat aattacagaa gttggtgtag gatacagatt
    21901 aaatgatgaa tagttgtaat atattaatta ttagattatg tgcttattaa tatatattaa
    21961 aattatatag tttagttaaa ataagaatga agtgtatgtg cttgttgtca gtttagcaca
    22021 tgcattttat tttttagctt ttttagtatc aataattttt tagtatcaat aatatgaaat
    22081 atgttttaaa ataagtatat agtgtaatat gtaataaaaa ctttggttaa ataat
//
LOCUS       DAMJYR010000055        21942 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.055, whole genome shotgun sequence.
ACCESSION   DAMJYR010000055 DAMJYR010000000
VERSION     DAMJYR010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 21942)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21942)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21942
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.055"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            370..681
                     /gene="rpsJ"
                     /locus_tag="PC471_002085"
     CDS             370..681
                     /gene="rpsJ"
                     /locus_tag="PC471_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HDF2789213.1"
                     /translation="MAKNEKIRIRLKSYDHKLLDFSAGKIVETAKKAGSQVSGPVPLP
                     TEKQVVTILRAVHKYKDSREQFEIRTHKRLIDIANPTPKTVDSLMRLDLPAGVDIEIK
                     L"
     gene            773..1402
                     /gene="rplC"
                     /locus_tag="PC471_002086"
     CDS             773..1402
                     /gene="rplC"
                     /locus_tag="PC471_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HDF2789214.1"
                     /translation="MKGILGKKVGMTQIFTDKGVVIPVTAVEAGPMVVTQIKTVDKDG
                     YNAIQIGFEDAKEKALNKPKKGHLAAANVLKKHLKEFRVDSVEGYTVGQEIKADVFEA
                     GAKIDVTGISKGKGFQGPIKRHGQSRGPETHGSRYHRRPGSMGACSYPGRVFKNKKLA
                     GHMGSVKVTVQNLEVVKVDADKNLILVKGAIPGAKGSVVTIKEAIKVSK"
     gene            1432..2055
                     /gene="rplD"
                     /locus_tag="PC471_002087"
     CDS             1432..2055
                     /gene="rplD"
                     /locus_tag="PC471_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001127258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HDF2789215.1"
                     /translation="MPKLNVLNVSGQNVGEIELSDSIFGVEVNGHVLYEVVKNQLANK
                     RQGTQSAKTRAEVRGGGRKPWKQKGTGRARQGSTRSVQWVGGGVAFAPKPRSYKYTLP
                     KKVRRLAMKSALSSKVQNSEVIVLDALNMDAPKTKEFAQILNNINAAKKALVVIADKN
                     DNVIKSARNIEGVQTALVNTMNVYDILKYDSFIITTDAVKKVEEVYA"
     gene            2055..2345
                     /gene="rplW"
                     /locus_tag="PC471_002088"
     CDS             2055..2345
                     /gene="rplW"
                     /locus_tag="PC471_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001205558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HDF2789216.1"
                     /translation="MTNPHDVIIRPVVTEHSMAEMGEKKYTFVVAKDANKTEIKKAVE
                     KVFGVSVDKVNTLNYDGKVKRMGRTQGRTSSFKKAVVKLTADSKEIEFFQGM"
     gene            2375..3205
                     /gene="rplB"
                     /locus_tag="PC471_002089"
     CDS             2375..3205
                     /gene="rplB"
                     /locus_tag="PC471_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HDF2789217.1"
                     /translation="MAIKKFRPTSPALRQMTVLVSDEITCNQPEKSLLVNLKKNAGRN
                     VHGRITVRHRGGGQKRKYRIIDFKRDKDGIPAKVATIEYDPNRTANIALLNYADGEKR
                     YILAPVGINVGDTILSGLGADIKPGNCLALKDMPVGTIIHNIELKPGKGAQLVRSAGV
                     SAQLMAKEGKNALLRLPSGEMRLVSINCKATIGQVGNIEHGNVVIGKAGRKRHMGIRP
                     TVRGSVMNPNDHPHGGGEGRSPIGRPSPVTPWGKPALGYKTRKKNKASNKLIVSRRTK
                     "
     gene            3240..3521
                     /gene="rpsS"
                     /locus_tag="PC471_002090"
     CDS             3240..3521
                     /gene="rpsS"
                     /locus_tag="PC471_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003454422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HDF2789218.1"
                     /translation="MSRSTKKGPFVHARLLKKIEAMNASGNKEVIKTWSRSSTVFPQM
                     VENTIAVHDGRKHVPVYITEDMVGHKLGEFVPTRTFKGHKDDEKSNKRK"
     gene            3553..3888
                     /gene="rplV"
                     /locus_tag="PC471_002091"
     CDS             3553..3888
                     /gene="rplV"
                     /locus_tag="PC471_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018590372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HDF2789219.1"
                     /translation="MEAKATAKYVRVSPRKAGQICDLVRGKNVDEALAILKFTPRGAA
                     SIIAKVVKSAKANAENNHEMDTEKLYIASIVANQGPTMKRFMPRAMGRATTIRKRTSH
                     IEVVVKEKK"
     gene            3911..4726
                     /gene="rpsC"
                     /locus_tag="PC471_002092"
     CDS             3911..4726
                     /gene="rpsC"
                     /locus_tag="PC471_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HDF2789220.1"
                     /translation="MGQKVNPHGLRVGVIKDWDSRWFATDKKEFGNLLLEDHNIRKFL
                     KKRLYSAGVAKIEIERSANKIKMDLHVAKPGVVIGRAGAGIEALKAELEKMTKKTIIV
                     NIVEVRSTDKNAQLVAENIALAIERRVAFRRAMKQAIQRAMKSGAKGIKVSASGRLGG
                     AEMARTEGYSEGNVPLQTLRADIDYGFAEADTTYGKIGIKVWICNGEVLPTRDGVNPR
                     EESRKSDRRDNKRDNRRNDRRGNDRRGNDNRGNYRGQRPQGGSRPQRTENKGN"
     gene            4762..5193
                     /gene="rplP"
                     /locus_tag="PC471_002093"
     CDS             4762..5193
                     /gene="rplP"
                     /locus_tag="PC471_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HDF2789221.1"
                     /translation="MLMPKRVKRRRVHRGSMAGQAHKGNKVTYGEFGLVALEASWITS
                     NQIEAARIAMTRYIKRGGKVWIKIFPHKPVTRKPAETRMGAGKGSPEYWVAVVKPGRV
                     MFELAGVSEDKAREAMRLAAHKLPIKCKFVKKEDLEVKGGE"
     gene            5195..5398
                     /gene="rpmC"
                     /locus_tag="PC471_002094"
     CDS             5195..5398
                     /gene="rpmC"
                     /locus_tag="PC471_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005810148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HDF2789222.1"
                     /translation="MKAKELRDLTSEELMNKLNDFKSELFSLRFQLATGQLENTARIK
                     FVKKDIAKVKTVLAERKLYETRA"
     gene            5422..5676
                     /gene="rpsQ"
                     /locus_tag="PC471_002095"
     CDS             5422..5676
                     /gene="rpsQ"
                     /locus_tag="PC471_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018590376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HDF2789223.1"
                     /translation="MERGRRKVRIGRVVSDKMDKTIVVAVEEFVRHPLYNKRVKRTKK
                     FKAHDEKNICNIGDRVKIMETRPLSKDKRFRLVEVVEKVK"
     gene            5702..6070
                     /gene="rplN"
                     /locus_tag="PC471_002096"
     CDS             5702..6070
                     /gene="rplN"
                     /locus_tag="PC471_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007885948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HDF2789224.1"
                     /translation="MIQQESRLRVADNSGAKELLCIRVLGGSKRRYGNIGDVIVATVK
                     SATPGGVVKKGKVVKAVIVRSKQGVRRNDGSYISFDENAAVIIKDDKTPVGTRIFGPV
                     ARELRDNEFMKIVSLAPEVL"
     gene            6094..6399
                     /gene="rplX"
                     /locus_tag="PC471_002097"
     CDS             6094..6399
                     /gene="rplX"
                     /locus_tag="PC471_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HDF2789225.1"
                     /translation="MRVKKGDTVVVIAGKDKGKKGSVLKVYPKTSKVLVEGVNVITKH
                     QKPSAMNQQGGIINKEAPIHISNVMPFDPETGKGVRVRYEVKDGNKVRVSAKSGKEL"
     gene            6429..6971
                     /gene="rplE"
                     /locus_tag="PC471_002098"
     CDS             6429..6971
                     /gene="rplE"
                     /locus_tag="PC471_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018590379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HDF2789226.1"
                     /translation="MASRLQEKYMKEVAPALMEKFGYKNVMEIPKLNKIVINMGIGDA
                     RENPKGLEKGVEELEMISGQKPVITKARKSVANFKLREGMPIGTKVTLRADKMFYFMD
                     KLVSVSLPRVRDFRGVNPNAFDGRGNYALGVKEQLIFPEIEYDKIDKVRGMDIIFVTT
                     AKTDEEARELLKLLGMPFSK"
     gene            6989..7174
                     /locus_tag="PC471_002099"
     CDS             6989..7174
                     /locus_tag="PC471_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HDF2789227.1"
                     /translation="MARKAMVVKQQRKQKYATREYTRCTICGRPHSVLKKFGICRICF
                     RELAYKGQIPGVRKASW"
     gene            7207..7605
                     /gene="rpsH"
                     /locus_tag="PC471_002100"
     CDS             7207..7605
                     /gene="rpsH"
                     /locus_tag="PC471_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012158382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HDF2789228.1"
                     /translation="MTMTDPIADMLTRIRNANVVKHETVDVPASNMKKELARILLEEG
                     FIRGYDVIEDGKQGIIRIQLKYGQEGERVITGLKKISKPGMRVYAANHEIPKVLNGLG
                     ISVISTSKGILTDKQARKENVGGEVICYVW"
     gene            7636..8178
                     /gene="rplF"
                     /locus_tag="PC471_002101"
     CDS             7636..8178
                     /gene="rplF"
                     /locus_tag="PC471_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HDF2789229.1"
                     /translation="MSRIGVKPIIIPAGVEVTIAEGNLVTVKGPKGTLTKQLSAELNI
                     KKEENTIMVERPTDNKKHRSLHGLTRTLLDNMVVGVNTGFEKKLELKGVGYRAQKQGK
                     KLVMNLGFSHPVEMEDPEGITVEAPNQTELIVKGIDKQLVGNYAAKIRAWRKPEPYKG
                     KGIKYVDEVIRRKEGKTGKK"
     gene            8214..8582
                     /gene="rplR"
                     /locus_tag="PC471_002102"
     CDS             8214..8582
                     /gene="rplR"
                     /locus_tag="PC471_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HDF2789230.1"
                     /translation="MLKKADKNANRLQRHKRVRRKISGTSQRPRLCVFRSANNIYAQI
                     IDDTKRVTLVAASSLEAEVKSAVNHTGNKEAAKKVGELVAKKAVEKGITEVVFDRGGY
                     LYHGRIQELAEGAREAGLKF"
     gene            8603..9112
                     /gene="rpsE"
                     /locus_tag="PC471_002103"
     CDS             8603..9112
                     /gene="rpsE"
                     /locus_tag="PC471_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011109776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HDF2789231.1"
                     /translation="MLRRKPIDAGQLDLQEKVVEVRRVTKVVKGGRNFRFAALVVVGD
                     ENGHVGIGAGKAMEVPDAIKKAVEDAKKNLIVVPIVGTTIPHEVRGHFGAGNILIMPA
                     VEGTGVIAGGPARAVLELAGLKDVRAKSLGSNNPRNMVNATIEGLNSLKTVEDIAKLR
                     GKKVEELLG"
     gene            9127..9312
                     /gene="rpmD"
                     /locus_tag="PC471_002104"
     CDS             9127..9312
                     /gene="rpmD"
                     /locus_tag="PC471_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HDF2789232.1"
                     /translation="MAKLQIKLVRSVIGTTPNQKKNVEALGLRKREQVVVKEDNAQTR
                     GMINKVSHLLEVTEIAE"
     gene            9345..9788
                     /gene="rplO"
                     /locus_tag="PC471_002105"
     CDS             9345..9788
                     /gene="rplO"
                     /locus_tag="PC471_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000766080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HDF2789233.1"
                     /translation="MKLHELKPAEGAVRAKRRLGRGTATGQGKTAGRGQKGQWSRSGG
                     GVRVGFEGGQMPLARRLPKRGFNNIFKKVYTEVNVEVLNRFENGTEITAELLKSTKTI
                     SKIGKDGIKILGEGNLEKALTVKAAKFTASAQEKIEKAGGKAELV"
     gene            9832..11100
                     /gene="secY"
                     /locus_tag="PC471_002106"
     CDS             9832..11100
                     /gene="secY"
                     /locus_tag="PC471_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HDF2789234.1"
                     /translation="MLSKLKQAWKIKAVRKKVMYTLMMIVIFRIGTTIPVPGIDTSII
                     QKMVGGNSLLSLYNMFTGGAFSNFSLFALGISPYITASIIIQLLTVGFESLAELQKSG
                     EEGKKKINKYTKYTALALAVVQALGITLGIVRSALISNSVFFITTVVITLVSASMLVM
                     WIGDKITEKGIGNGSSVIIFAGIISRIPTDVIKISQQVKSGEVAPWVIVILAVVILLT
                     VTGVTFIQEATRKIPVQYAKRVVGRKMYGGQSSHIPMKVNQSGVMPIIFASSLLAFPQ
                     TIAMFMGPNAQAFVQKYLSMATEQGFWTYRSIEILLIIFFSYFYTTVSFNTEDISKNM
                     KNNGGFIPGIRPGEPTMDYLNRILTRLTLAGATFLAIIAMVPALTTHYMKVNMSLAGT
                     SLLIVVGVALELKRQLESNLVMRSYQGFLK"
     gene            11117..11767
                     /locus_tag="PC471_002107"
     CDS             11117..11767
                     /locus_tag="PC471_002107"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HDF2789235.1"
                     /translation="MRIILLGPPGAGKGTQAAGIVEKYNIPHISTGDIFRKNIKEGTE
                     LGKKAKEYMDQGLLVPDELTVGLVTDRISQEDCKNGFMLDGFPRNVAQGEHLDIFLKN
                     AGISLDKVVNIEVDKSILVSRAVGRRICKSCGATYHVEFNPPKVEGVCDVCQGELYQR
                     ADDNEETVSKRIQVYLDETKPLVDYYSKQGIIADIKGDQAIDKVFEDIVAALGSGK"
     gene            11768..12514
                     /gene="map"
                     /locus_tag="PC471_002108"
     CDS             11768..12514
                     /gene="map"
                     /locus_tag="PC471_002108"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HDF2789236.1"
                     /translation="MIILKSKKEIELLREAGKIVADTHEVLRKAISPGISTLELDKIA
                     EENIRKYNAEPSFKGYGGFPGSICASINREVVHGIPGETILQEGDIVSLDIGAYYKGY
                     HGDSAKTHGVGMISEEDRKLIEVTRESFYEGIKFAKLGYRLSDISHAVQTHVEKHGFS
                     VVRDLVGHGVGANLHEDPQVPNYGLPGKGPRLREGMVIAIEPMVNAGRYHVKTLSDGW
                     TTVTIDGKKSAHYEHTIAITEHEPLILTKL"
     gene            12536..12805
                     /locus_tag="PC471_002109"
     CDS             12536..12805
                     /locus_tag="PC471_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KOW domain-containing protein"
                     /protein_id="HDF2789237.1"
                     /translation="MLSDNLSIGQVVKVSLGRDKGNLFFVVKIINNEYVLIADGKKRK
                     LDKPKLKKVKHLKKYNFINDEVRKRVVSGQEITDSFLRAELTKLN"
     gene            12843..13061
                     /gene="infA"
                     /locus_tag="PC471_002110"
     CDS             12843..13061
                     /gene="infA"
                     /locus_tag="PC471_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HDF2789238.1"
                     /translation="MAKKDVIELEGTVSEALPNAMFKVKLENGHEILCHISGKLRMNF
                     IRILEGDKVNVELSPYDLTRGRITWRKK"
     gene            13097..13210
                     /gene="rpmJ"
                     /locus_tag="PC471_002111"
     CDS             13097..13210
                     /gene="rpmJ"
                     /locus_tag="PC471_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HDF2789239.1"
                     /translation="MKVRPSVKPICEKCKVIKRKGKVMVICENPKHKQKQG"
     gene            13363..13734
                     /gene="rpsM"
                     /locus_tag="PC471_002112"
     CDS             13363..13734
                     /gene="rpsM"
                     /locus_tag="PC471_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005810116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HDF2789240.1"
                     /translation="MARIAGVDLPREKRAEIGLTYIYGIGKATANEILAKAEINPDTR
                     IKDLSEDQVNELRKVIDDDFLVEGDLRREIALNIKRLRDIKCYRGIRHAKGLPLRGQR
                     TKTNARTRKGPRKTVSRKKKK"
     gene            13770..14168
                     /gene="rpsK"
                     /locus_tag="PC471_002113"
     CDS             13770..14168
                     /gene="rpsK"
                     /locus_tag="PC471_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006440523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HDF2789241.1"
                     /translation="MAKPKKKVTRIRRRERKNIERGHAHIQSTFNNTIITLTDVHGNA
                     ISWASSGQLGFKGSRKSTPFASQMAAETAAKAAMEHGLKSVEVFVKGPGSGREAAIRA
                     LQATGLEVTMIKDVTPIPHNGCRPPKRRRV"
     gene            14200..14823
                     /gene="rpsD"
                     /locus_tag="PC471_002114"
     CDS             14200..14823
                     /gene="rpsD"
                     /locus_tag="PC471_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HDF2789242.1"
                     /translation="MARYTGASCRQCRREGMKLFLKGDRCYTDKCAIVKRNYAPGQHG
                     QGRKKVSNYGLQLREKQKVKRIYGVLETQFRNLYERAENMPGKAGENLLSLLERRLDN
                     VVYRMGLASSRKEARQLVTHGHFTLNGNKVDIPSLIVKVGDVIEVKEKSRSSAKFKNL
                     VEVNSRIAPKWLEANVEGMTAKVVGVPTREDIDLEIAEHLIIELYSK"
     gene            14901..15848
                     /locus_tag="PC471_002115"
     CDS             14901..15848
                     /locus_tag="PC471_002115"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HDF2789243.1"
                     /translation="MIEIEKPKVDIVELSEDYRYGKFVIEPLERGYGITIGNALRRIL
                     LSSLPGVAVNAIKIDGVLHEFSTIPGVKEDVTEIILTLKELSATIDGEGSRTLKIEAQ
                     GPCSITGADIICPPDVEILSKDLAIATLDDNAKLNMEIFVDKGRGYVSAEENKTENVP
                     IGVLPVDSIYTPVEKVSYHVENTRVGQKTDYDKLVLEVWTNGSINPQEGISLAAKVLV
                     EHLNLFIDLTEHVSSVEIMVEKEEDQKEKVLEMTIEELDLSVRSYNCLKRAGINTVEE
                     LANKSEDDMMKVRNLGKKSLEEVIQKLEELGLGLKPSEE"
     gene            15869..16210
                     /gene="rplQ"
                     /locus_tag="PC471_002116"
     CDS             15869..16210
                     /gene="rplQ"
                     /locus_tag="PC471_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003357477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HDF2789244.1"
                     /translation="MAKYRKLGRETAHRNLMLRNLVTCLLRSGRIETTVTRAKETRRM
                     AEKMITLAKRGDLHARRQVLAYVMDETVVNNLFTDLAPKYAERNGGYTRIIKIGPRKG
                     DAAEMAFIELV"
     gene            16330..17163
                     /locus_tag="PC471_002117"
     CDS             16330..17163
                     /locus_tag="PC471_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HDF2789245.1"
                     /translation="MDNIVKVNNISFEYITDEAKLKAIDNLSLDVKKGEFVAIIGHNG
                     SGKSTLSKNLNAILMPTEGNILIDDMDTKEEERLWDIRQTAGMVFQNPDNQIVATIVE
                     EDVAFGPENLGIEPKEIRRIVEESLKSVGMYDLRDRQPHLLSGGQKQRVAIAGIIAMR
                     PKCIIFDEATAMLDPSGRKEVMKTIKRLNKEENITVIHITHFMEEAVEADRVVVMEKG
                     KKILEGTPREVFSKIKMLKEIGLDVPCMTELSSLLIEEGINISSDILTVDEMVMELCQ
                     L"
     gene            17151..18017
                     /locus_tag="PC471_002118"
     CDS             17151..18017
                     /locus_tag="PC471_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HDF2789246.1"
                     /translation="MSIIVKNLTHIYNEGMPFASKALDDVSFEIKDRDFVGLIGHTGS
                     GKSTLIQHLNGLLKPSSGEIFINDFNITDKNLNLTEIRKRVGVVFQYPEYQLFEETID
                     KDIAFGPSNLGLEESEIHNRVKASMEAVGLDYEGFKDKSPFELSGGQKRRVAIAGVIA
                     MNPEVLILDEPTAGLDPGGRDEIFNLIKDLHEKKNMTIILSSHSMDDMAKLAKTLIVM
                     NHGSVEFMGTPREVFKSNASKLKDIGLDIPQVLELALKLREKGFDISEDILTLEEAKQ
                     EILKVVRGRGLC"
     gene            18011..18814
                     /locus_tag="PC471_002119"
     CDS             18011..18814
                     /locus_tag="PC471_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HDF2789247.1"
                     /translation="MLKDITIGQYYPTSSAIHKLDPRIKLVATIVFMVSIFVVNKFWP
                     YIVVLLCLLAMIKLANIPVKYIVKGVKPLKWIILFTFLINIFFLPGDEIWSFGFLAIT
                     KQGLRQAIFMAIRLIFLVVGTSLLTLTTSPIELTDGIERLLNPFKKIGLPVHELAMMM
                     TIALRFIPTLLDETDKIMKAQMSRGADFESKNLINRAKNLVPLLVPLFVSAFRRADEL
                     AMAMEARCYRGGHNRTKMRESVISRRDYMACVFQVVYLGAIIATRFIAI"
     gene            18846..19577
                     /gene="truA"
                     /locus_tag="PC471_002120"
     CDS             18846..19577
                     /gene="truA"
                     /locus_tag="PC471_002120"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HDF2789248.1"
                     /translation="MRNIKIKIQYNGKNYCGWQKQPDSLGIQGTIERAIYDITKEETS
                     LIGSGRTDSGVHAIGQIANFKINSGISIESIPMALNAKLPKDISVIEACEVNDDFHSR
                     YSAKGKTYKYLVYNSKFRNPILSEISYQVKYELDFDKMCSEAKSLLGTHDFKGFMSSG
                     SSVKDTVRTIYDIDISKKDDLITFEISGNGFLYNMVRIIVGTLVDMGRGRINEPFLDI
                     IQSKTRSRCGHTAPAQGLFLKKVHY"
     gene            19697..20128
                     /gene="rplM"
                     /locus_tag="PC471_002121"
     CDS             19697..20128
                     /gene="rplM"
                     /locus_tag="PC471_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HDF2789249.1"
                     /translation="MKSYIAKPADVQRKWYLVDAEGKTLGRLATEIATVLRGKHKPTF
                     TPHVDGGDFVVVVNAEKIVLSGKKLDQKYYRYHTGYVGGLKEISYRDMMDKKPEEVIS
                     HAVSGMLPKNKLRSRMMTRLRVFAGAEHTHAAQNPEVLNFK"
     gene            20157..20549
                     /gene="rpsI"
                     /locus_tag="PC471_002122"
     CDS             20157..20549
                     /gene="rpsI"
                     /locus_tag="PC471_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HDF2789250.1"
                     /translation="MANVQYYGTGRRKSSVARVRLVAGEGNILVNGRALENYFNYETL
                     IRDVKQPLVLTGNENKYDVIVKVEGGGFTGQAGAIRHGISRALLKADLDLRPALKKEG
                     FLTRDARMKERKKYGLKAARRAPQFSKR"
     gene            21008..21760
                     /gene="cwlD"
                     /locus_tag="PC471_002123"
     CDS             21008..21760
                     /gene="cwlD"
                     /locus_tag="PC471_002123"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase CwlD"
                     /protein_id="HDF2789251.1"
                     /translation="MEYYSVFISIHIQGDVVRKYIKHIIFSFAMICLVVVSIFEIKNI
                     SEDVIKYMPVTNKTIILDAGHGGIDPGALNKDKSTSEKDINLAITLKLRELIESSGGL
                     VILTREDDSSLYKEENNKTTRQKYNENLKNRKEIISNSNANMFVSIHLNAFEQSKYYG
                     AQTFYPKDKQDSKELSKCIQEELKRVVDKTNNREVKPRDDIYLLKENNIPSVLIECGF
                     LSNEKECKLLTDETYQEKIAWAIYIGIQKYLS"
BASE COUNT         8816 a         2563 c         4494 g         6069 t
ORIGIN      
        1 ctttactaaa ctaatggatt taatataaaa aaaagagggg gtaactcctc tcttttttgt
       61 tttttgttga caagtagcgc taaagcatat atactaaact agtgccttga gacagattaa
      121 caatcaagtt taaggttatg atgttgtaat agaaaataaa attgaaaaaa acagaaaaaa
      181 aacttgaaaa aatgtaacag atgatataaa atataatagt gtgcttgaga gattaagaaa
      241 ataataatta aatcctagtg aaatacgaac tgatatcgat ttaaaagtta caacacaccc
      301 ggagcagtgt atcggtattg gttgtacgca acttgaaaat taaacacgtg ataaaggagg
      361 gaaaacaaaa tggctaagaa tgaaaaaata agaataagat taaaatcata tgatcacaaa
      421 ttattagatt tttcagcagg taaaatagtt gaaactgcta aaaaggctgg atcacaagtt
      481 tcaggacctg tgcctctacc aacagaaaaa caagttgtaa ctatattaag agctgtacat
      541 aagtacaaag actctagaga acaatttgaa ataagaactc ataaaagact aatcgacata
      601 gctaatccaa cacctaagac agttgactca ttaatgagat tagacttacc agcaggtgta
      661 gatatagaaa taaaattata agaaccccta agatattatc ctatagatga ttatcttaga
      721 atgattactc taaaagatgt aatccgctgt aagaattata ggaggtgcta atatgaaagg
      781 aatattaggc aaaaaagttg gtatgactca aatattcact gataaaggtg tagttatacc
      841 tgtaacagct gttgaagcag ggcctatggt cgttactcaa ataaaaacag ttgataaaga
      901 tggatacaat gctatacaaa taggttttga agatgctaaa gaaaaagctt taaacaaacc
      961 taaaaaagga catttagctg ctgctaatgt tttaaagaaa catttaaaag aatttagagt
     1021 agattctgta gaaggataca cagtaggaca agaaataaaa gctgatgtat ttgaagcagg
     1081 tgcaaaaata gatgttactg gaataagtaa aggtaaggga ttccaaggtc caataaaaag
     1141 acatggacaa agtagaggtc ctgaaactca cggttctaga taccacagaa gaccaggttc
     1201 aatgggagct tgttcttacc caggtagagt atttaaaaac aaaaaattag ctggacatat
     1261 gggaagcgta aaggtaacag ttcaaaactt agaggttgtt aaagtagatg ctgacaagaa
     1321 ccttatatta gttaaaggag ctataccagg agctaaaggt tcagtagtaa ctataaaaga
     1381 agctataaag gtttctaaat aatgactaac ctagagaaag gaggaataac aatgccaaaa
     1441 ttaaatgtac taaatgttag tggacaaaac gttggagaaa tagaattatc agattctata
     1501 tttggcgtag aagttaatgg acatgtttta tacgaagttg taaaaaatca attagcaaat
     1561 aagagacaag gaactcaatc tgctaaaact agagcagaag ttagaggtgg cggaagaaaa
     1621 ccttggaaac aaaaaggaac aggtagagca agacaaggtt ctacaagatc tgtacaatgg
     1681 gtaggtggag gagttgcttt tgcacctaaa ccaagaagct acaaatatac attacctaag
     1741 aaggtaagaa gattagctat gaagagtgct ttatcttcaa aagttcaaaa tagtgaagtt
     1801 atagtattag atgcattaaa catggatgct cctaagacta aagaatttgc tcaaatatta
     1861 aacaatataa atgctgctaa aaaagcttta gtagttatag ctgacaaaaa cgataacgta
     1921 ataaaatcag ctagaaatat agaaggtgtt caaactgcat tagttaatac tatgaatgta
     1981 tatgatatat taaaatacga ttcatttata ataactacag atgctgttaa aaaagtggag
     2041 gaggtgtacg cataatgact aatccacatg atgtaataat aagaccagtt gtaactgagc
     2101 acagtatggc tgaaatgggt gaaaaaaaat acacttttgt tgtggctaaa gatgcgaaca
     2161 aaactgaaat aaaaaaagca gtagaaaaag tattcggagt tagtgttgat aaagtaaata
     2221 ctttaaacta cgatggaaaa gttaaaagaa tgggtagaac tcaaggaaga acatcaagct
     2281 ttaagaaagc agtagttaaa ttaactgctg atagtaaaga aatagaattc ttccaaggaa
     2341 tgtagtatta taacaaacga aggagggaaa acagatggct ataaaaaagt ttagaccaac
     2401 ttctcctgcc ttaagacaaa tgacagtttt agtttctgat gaaataacat gtaatcaacc
     2461 agaaaaatct cttttagtta acttaaagaa aaatgctggt agaaacgtac atggtagaat
     2521 aactgttcgt catagaggtg gaggacaaaa aagaaaatat agaataatag attttaaaag
     2581 agataaagat ggtatacctg ctaaggttgc tactatagaa tatgatccaa acagaactgc
     2641 aaatatcgca ttattaaact atgctgatgg agaaaaaaga tatatattag ctccagttgg
     2701 aattaacgtt ggagatacta tattatcagg attaggtgct gatataaaac caggaaactg
     2761 tttagcatta aaagatatgc cagttggtac tatcattcat aatatagaat taaagccagg
     2821 aaaaggagct caattagtta gatctgctgg agtttctgca caattaatgg ctaaagaagg
     2881 aaaaaatgct ttattaagat taccatcagg tgagatgaga ttagtaagca taaactgtaa
     2941 agctactata ggtcaagttg gaaatataga gcatggtaac gtagttatcg gtaaagcagg
     3001 tagaaaaaga catatgggaa taagacctac tgtaagaggt tctgtaatga accctaatga
     3061 ccatccacac ggtggagggg aaggtagatc tccaataggt agaccatctc ctgttactcc
     3121 atggggtaaa ccagctcttg gatacaaaac tagaaagaaa aataaagctt caaataaact
     3181 aatagtatca agaagaacta agtagtaaat ttatttttgc tcggaaagga ggaatataaa
     3241 tgtcaagatc aactaaaaaa gggccttttg tccatgcaag acttttaaag aagatagaag
     3301 ctatgaatgc tagtggaaat aaagaagtta taaaaacttg gtcaagatct tcaactgtat
     3361 ttccacaaat ggtagaaaac actatagctg ttcatgatgg aagaaaacat gttccagtat
     3421 atataacaga agatatggtt ggacataaat taggtgagtt cgttcctact agaactttca
     3481 aaggacataa ggatgacgaa aaatctaata agagaaaata attaaattat gactaagaaa
     3541 ggaggaataa caatggaagc aaaagctact gctaaatacg tacgtgtatc accaagaaaa
     3601 gcaggccaaa tatgtgacct tgttagagga aagaatgttg atgaagcatt agcaatatta
     3661 aagtttactc caagaggagc agcttcaata atagctaagg ttgtaaaatc agctaaagct
     3721 aacgcagaaa acaatcacga aatggatact gaaaaattat atatagcatc aatagttgct
     3781 aatcaaggac caacaatgaa gagattcatg cctagagcta tgggtcgtgc aactacaata
     3841 agaaaaagaa cttctcatat agaggttgtt gttaaagaaa aaaaataatt aatagatagg
     3901 agggaaagta atgggtcaaa aggttaatcc acacggctta agggtcggtg ttataaaaga
     3961 ttgggactca agatggttcg ctactgataa aaaagagttc ggaaacttat tattagaaga
     4021 ccataatata cgtaaattct taaagaaaag attatactca gctggagttg ctaagataga
     4081 aatagaaaga tcagctaaca aaataaaaat ggacttacac gttgctaagc ctggtgtagt
     4141 tataggaaga gctggtgcag gaatcgaagc attaaaagct gaattagaaa aaatgacaaa
     4201 gaaaactata atagttaata tagtagaagt aagaagtaca gataaaaatg ctcaattagt
     4261 agctgagaat atagctttag ctatagagag aagggttgct ttcagaagag caatgaaaca
     4321 agctatacaa agagcaatga aatctggtgc taaagggata aaagtttctg cttcaggaag
     4381 attaggtgga gctgaaatgg ctagaactga aggttatagt gaaggaaatg tgccactaca
     4441 aactttaaga gcagatatag attatggttt cgctgaagct gatacaactt atggaaaaat
     4501 cggtataaaa gtttggatat gtaatggtga agttttacca actagagacg gtgtaaatcc
     4561 aagagaagaa agcagaaaga gcgatagaag agacaacaag agagataata gaagaaacga
     4621 tagaagagga aatgacagaa gaggaaatga taacagagga aactacagag gacaaagacc
     4681 tcaaggtgga tcaagacctc aaagaactga aaacaaagga aattaataat agttaggttc
     4741 aaggaaggag gaaaaatact catgttaatg ccaaaaagag taaagcgtcg tagagttcat
     4801 agaggaagca tggctgggca agctcataag ggtaataaag ttacttatgg tgagtttgga
     4861 ttagtagcat tagaagcttc ttggataact tctaatcaaa tagaagctgc cagaatcgcg
     4921 atgactagat atataaaaag agggggaaag gtttggataa aaatattccc tcataaacca
     4981 gtaacaagaa aaccagcaga gactcgtatg ggtgcaggga aaggttctcc agaatattgg
     5041 gtagctgtag ttaagccagg aagagttatg tttgaattag caggtgtttc tgaagataaa
     5101 gcaagagaag ctatgagact tgctgcacat aaattaccaa tcaaatgtaa atttgttaaa
     5161 aaagaagatt tagaagtaaa gggtggtgaa taggatgaaa gctaaagaat taagagattt
     5221 aacaagcgaa gagctaatga acaaattaaa cgacttcaaa agtgaattat ttagcttaag
     5281 attccaatta gctactggtc aattagaaaa tacagctaga ataaagtttg ttaagaagga
     5341 tatagcaaaa gttaaaactg ttcttgctga aagaaagtta tacgaaacta gagcttaatt
     5401 tggaaaggag gctttttaaa catggaaaga ggaagaagaa aagttagaat aggccgtgtt
     5461 gttagtgata agatggataa aacaatagta gtagctgttg aagaattcgt acgtcatcca
     5521 ctatataata aacgtgttaa gagaactaag aaattcaagg ctcatgatga aaagaatata
     5581 tgtaacatcg gagatagagt taaaataatg gaaactagac cattatctaa agataagaga
     5641 ttcagactag ttgaagttgt tgagaaagtt aagtagtttt tgaagaagga gggattacga
     5701 tatgatacaa caagaatcac gtctaagagt tgctgataac tcaggagcta aagaactttt
     5761 atgtatacgt gttctaggcg gaagtaaaag aagatatggt aacataggcg acgttatagt
     5821 tgctactgtt aaaagtgcaa cacctggtgg agttgtaaaa aaaggtaagg ttgttaaagc
     5881 tgttatagta agaagcaaac aaggcgtaag acgtaatgac ggaagctata tatcttttga
     5941 tgaaaatgct gctgttataa taaaggacga taaaactcca gtaggaactc gtatattcgg
     6001 gcctgttgct agagagttaa gagacaatga attcatgaaa atagtttctc ttgctccaga
     6061 agtactataa ttaaggaggt gcaataggac atgatgcgtg ttaaaaaagg tgacactgtt
     6121 gtagttatag caggtaaaga taaaggtaaa aaaggttcag ttttaaaggt atatcctaaa
     6181 actagcaaag tgttagttga aggtgtaaat gtaataacta aacatcaaaa accaagtgct
     6241 atgaaccaac aaggtggaat aataaataaa gaagccccaa tacacatatc taatgtaatg
     6301 ccatttgatc ctgaaacagg aaaaggcgtt agagtaagat atgaagtaaa agatgggaac
     6361 aaagtaagag tatcagcaaa gagcggaaaa gaattataat aaatattgct tgaaaggagg
     6421 gaccttaaat ggcttctaga ttacaagaaa aatacatgaa agaagttgct cctgctttaa
     6481 tggagaaatt tggatacaaa aacgtaatgg agatacctaa gttaaataaa atagttatta
     6541 acatgggtat aggtgacgct agagaaaatc caaaaggatt agaaaaaggc gttgaagaat
     6601 tagaaatgat atcaggacaa aagcctgtta taactaaagc tagaaaatct gttgctaact
     6661 tcaaattaag agaaggaatg ccaataggaa caaaagttac attaagagct gacaaaatgt
     6721 tctactttat ggacaaatta gtttcagttt ctttaccaag agttagagac tttagaggag
     6781 ttaaccctaa tgcttttgat ggtagaggaa attatgcttt aggagttaaa gaacaattaa
     6841 tattccctga aatagaatat gataaaatag ataaagtaag aggaatggat ataatatttg
     6901 ttactacagc aaaaactgac gaagaagctc gtgaattatt aaaattatta ggaatgccat
     6961 tttctaagta agcaaaggag ggattcccgt ggctagaaaa gcgatggttg ttaagcaaca
     7021 aagaaagcaa aagtacgcta ctagagaata cactagatgt actatatgcg gaagaccgca
     7081 ttcagttttg aaaaaatttg gtatatgccg tatatgcttt agagaattag cttataaagg
     7141 tcaaatacct ggtgttagaa aagcaagctg gtagaagctt gattagaatg gaaggagggc
     7201 aacaaaatga caatgacaga tccaattgca gatatgctta ctcgtataag aaatgctaat
     7261 gttgttaagc atgaaactgt agatgttcca gcttctaata tgaagaaaga attagctaga
     7321 atcttattag aagaaggttt cataagaggt tatgatgtta tagaagatgg aaaacaaggt
     7381 atcataagaa tacaattaaa gtacggacaa gaaggcgaaa gagttataac aggtcttaag
     7441 aaaatatcta agcctggaat gagagtttac gctgctaacc atgagatacc aaaagtatta
     7501 aacggattag gaatatcagt tatatctact tcaaaaggaa tattaactga caagcaagct
     7561 agaaaagaaa atgttggtgg agaagtaatc tgctacgttt ggtaattaat atgaacaatg
     7621 taaggaggtg cgaccatgtc aagaataggt gttaagccaa taataatacc agcaggtgtt
     7681 gaagttacta tagctgaagg aaatttagtt acagtaaaag gtccaaaagg aactttaact
     7741 aaacaattaa gtgctgagtt aaacataaaa aaagaagaaa atacaataat ggttgaaaga
     7801 ccaactgata ataaaaaaca tagatcttta cacggactaa ctagaacttt attagataat
     7861 atggttgtag gtgtaaatac tggtttcgaa aagaaattag aattaaaagg tgttggatat
     7921 agagctcaaa aacaaggaaa gaaattagtt atgaacttag gtttctctca tccagttgag
     7981 atggaagatc cagaaggaat aactgttgaa gctccaaacc aaacagagtt aatagtaaaa
     8041 ggtatagaca agcaattagt agggaattat gctgctaaga taagagcttg gagaaagcca
     8101 gagccataca aaggtaaagg tataaaatac gttgatgaag taatcagacg taaagaagga
     8161 aaaactggta aaaaataata ccaaaactta aacgctagaa aggagtgagc tagatgttaa
     8221 aaaaggctga taaaaatgct aatagacttc aaagacataa gagagttcgt agaaaaataa
     8281 gcggtacttc tcaaagacca agattatgtg tatttagaag tgctaacaat atatatgctc
     8341 aaataataga tgacacaaaa agagtaactc tagttgctgc atcttcttta gaagcagaag
     8401 ttaagagtgc tgtcaatcat acaggaaaca aggaagcagc taagaaagtt ggagaattag
     8461 ttgctaaaaa agctgttgaa aaaggaatca ctgaagttgt atttgacaga ggtggatact
     8521 tatatcatgg aagaattcaa gaattagctg aaggtgctag agaagctggt cttaagttct
     8581 aatacgaagg agggaaaaat acatgctacg tcgtaagccg atagatgcag gacaacttga
     8641 cttacaagaa aaagttgttg aagtaagacg tgttactaag gttgtaaaag gtggtagaaa
     8701 tttcagattt gcagcattag tagttgttgg agatgaaaac ggacacgttg gtataggtgc
     8761 tgggaaagca atggaagtac cagacgctat aaaaaaagca gttgaagatg ctaagaaaaa
     8821 tctaatagta gtgccaatag ttgggactac aattcctcac gaagtacgcg gacactttgg
     8881 tgctggaaat atattaataa tgcctgctgt agaaggtaca ggagtaatag ctgggggacc
     8941 tgctagagcc gtacttgagc tagctggact aaaagacgtt agagctaaat ctttagggtc
     9001 taacaatcca agaaatatgg taaatgctac aatagaagga cttaattctc taaaaacagt
     9061 tgaagatata gctaaactta gaggtaaaaa agttgaagaa cttctagggt aaggaggtag
     9121 tgtaaaatgg ctaaattaca aataaagtta gttagaagtg ttataggaac tactcctaac
     9181 caaaaaaaga atgttgaagc gttaggatta agaaaaagag aacaagttgt tgtaaaagaa
     9241 gacaatgctc aaacaagagg tatgataaat aaagtaagtc acttattaga agtaactgaa
     9301 atagctgaat aatttttata aagtagtgca aggaggtgca atccatgaag ttacatgagt
     9361 taaaacctgc tgaaggtgca gtaagagcta agagaagatt aggtagaggt actgcaactg
     9421 gacaaggtaa aactgcagga cgtggacaaa aaggtcaatg gtctcgttct ggtggtggag
     9481 taagagtagg atttgaaggt ggacaaatgc cactagctag aagacttcct aagagaggtt
     9541 tcaataacat atttaagaaa gtttacacag aagttaatgt tgaagtttta aacagatttg
     9601 aaaatggaac agaaataact gcagaattat taaaatctac taaaactata agcaaaatag
     9661 gtaaagacgg aattaaaatc ttaggtgaag gtaatttaga gaaagcttta actgttaaag
     9721 ctgctaaatt tacagcttca gctcaagaaa aaatagaaaa agctggtgga aaagcagagt
     9781 tagtataata acctgctaat ctcctagcca gtacaggcgg ggtgaattag cgtgctgtca
     9841 aaattaaaac aagcttggaa gattaaagca gtaagaaaaa aagtaatgta tacacttatg
     9901 atgatcgtga tattcagaat aggtactact ataccagttc caggcataga cactagtatt
     9961 atacaaaaga tggtaggcgg caatagtcta ctttctctgt ataatatgtt tactggtgga
    10021 gcatttagta acttttcact atttgcttta ggtataagtc catatatcac tgcatcaatc
    10081 ataatacaac ttctaacagt aggttttgaa agtttagcag aacttcaaaa atctggtgaa
    10141 gaaggaaaga aaaaaatcaa taagtacact aaatatacag cactagctct agcagttgta
    10201 caagctttgg gaattacatt aggaattgta agaagtgctt taatatcaaa tagtgtgttt
    10261 tttatcacaa ctgttgtaat aacattagtt tcagcaagta tgttagtaat gtggattggg
    10321 gacaaaatta ctgaaaaagg aatcggtaat ggtagttcag taataatatt tgcaggtata
    10381 atatcaagaa tacctacaga tgttattaaa atatcgcaac aagttaaatc tggcgaagtt
    10441 gcaccttggg ttatagtgat tctagctgta gttatattac ttacagtaac aggagttaca
    10501 tttatacaag aagcaactag aaagatacct gtacaatatg ctaaaagagt tgttggaaga
    10561 aaaatgtatg gaggacaaag ttctcatata ccaatgaaag ttaaccagtc aggagttatg
    10621 cctataattt ttgctagttc acttttagct ttccctcaaa caatagctat gtttatgggt
    10681 ccaaatgctc aggcttttgt tcaaaaatac ttaagtatgg caacagaaca aggattttgg
    10741 acatatagat caattgagat tctacttata attttcttct catattttta tacaactgta
    10801 tcatttaata cagaagacat atctaaaaat atgaaaaata atggtggatt tataccaggt
    10861 ataagaccag gtgagcctac tatggattat ttaaatagaa ttttaactag attaacttta
    10921 gctggagcta ctttcttagc aataattgct atggtgccag ctcttacaac acattatatg
    10981 aaagtaaata tgagtctagc tgggacttca ttgctaatcg ttgttggagt tgctttggag
    11041 cttaagagac agttagagtc aaacttagtt atgagaagct atcaaggttt cttaaaataa
    11101 aatggagatg gataatatga gaataatatt acttggacct ccaggtgctg gtaaaggtac
    11161 tcaggcggca ggaatagtag aaaaatacaa tatacctcat atatcaacag gagatatatt
    11221 cagaaagaat ataaaagagg gaacagaact tggaaaaaaa gctaaagaat acatggacca
    11281 aggtttatta gtaccagatg agttaactgt aggtttagtt actgatagaa tatctcaaga
    11341 agattgtaaa aatggattta tgttagatgg atttccaaga aatgtagcac aaggagaaca
    11401 tttagatatc ttcttaaaaa atgctggtat atcactagat aaagttgtca atattgaagt
    11461 tgataagagt atattagtgt ctagagcagt tggtagaaga atatgtaagt cttgtggagc
    11521 tacttaccat gttgagttta atcctcctaa agtagaaggt gtatgtgatg tatgccaagg
    11581 agaattatat caaagagctg atgataatga agaaactgta tctaagagaa tacaagttta
    11641 tctagatgaa actaagcctt tagtagatta ttatagcaaa caaggtataa tagcagatat
    11701 aaaaggtgat caagcaatag ataaagtatt tgaagatatt gtcgcagcct taggaagtgg
    11761 aaaataaatg attatcttga aatcaaaaaa agaaatagaa cttttaagag aagcaggtaa
    11821 aatagttgct gacactcatg aagttttaag aaaagctatt tctccgggaa tatctacttt
    11881 agaattagat aaaatagctg aagagaatat aagaaaatac aatgcagaac cttcttttaa
    11941 aggatatgga ggttttccag gaagtatatg tgcttctatc aatagagagg tagtacatgg
    12001 tatcccagga gaaactatat tacaagaagg tgacattgta agtttggata taggagccta
    12061 ttataaagga tatcatggtg attctgcgaa aactcacgga gtaggcatga tatctgaaga
    12121 agataggaaa ttgatagaag taactagaga aagcttctat gaaggaataa aatttgctaa
    12181 attaggatat agactttcag atatctcaca cgcagtacaa acacacgttg agaaacatgg
    12241 tttctcggta gttagagatt tagtgggtca tggagttgga gcaaatcttc atgaagaccc
    12301 acaagtacct aattatggac tcccaggaaa aggaccaaga cttagagaag gaatggttat
    12361 tgctatagag ccaatggtta atgcaggtcg ttatcatgta aagactctat cagatgggtg
    12421 gacgactgtt acaatagatg gaaaaaaatc agctcattat gagcatacga tagcaattac
    12481 tgaacatgaa cctttgatat tgacaaagtt gtaatttaaa aagtaggtga aaaaagtgct
    12541 atcagataat ttaagtatag gtcaagttgt taaagtttca ttaggacgag ataaaggaaa
    12601 tttattcttt gtagtcaaaa taattaataa tgaatatgta ttaatagctg atggaaaaaa
    12661 gagaaaactt gacaaaccta agttaaagaa agtgaagcac ttaaaaaaat ataattttat
    12721 taatgatgaa gttagaaaaa gagtagtatc tggacaagag attacagatt cttttttaag
    12781 agctgaactt actaagttaa attagataat ttaacttatt gaaatggagg gctggttagt
    12841 taatggccaa aaaagatgtt atagaattag aaggtacagt ttcagaagct ttacctaatg
    12901 ctatgttcaa agttaaacta gaaaatggac atgagatatt atgccacatt tctggaaagc
    12961 taagaatgaa cttcataaga attcttgaag gtgacaaggt gaatgttgaa ctttctccat
    13021 atgaccttac aagaggaaga attacttggc gtaagaagta gactatatta atatttagtc
    13081 taaggaggga ttaataatga aggttagacc atcagtaaaa ccaatatgtg aaaaatgtaa
    13141 agtaataaaa agaaaaggaa aagtaatggt tatctgtgaa aatccaaagc ataagcaaaa
    13201 gcaaggataa gaaattatta ctagcatttt gttaaatttt atagtataat actatattgt
    13261 gatgttaaca aaagaacgtt aaaatcgttg aacaatctgg cgtaggattg gcatatgatg
    13321 tgaagtttta aacactaaaa actatgtgta ggaggtgcga tcatggcaag aatagctggg
    13381 gtagatttac ctagagaaaa aagagcggag ataggcttaa cttatatata tggtataggt
    13441 aaagcaactg ctaacgaaat attagctaag gctgagataa atccagatac tagaataaaa
    13501 gatttatcag aagatcaagt taatgagtta agaaaagtta tagatgacga tttcttagtt
    13561 gaaggtgact taagaagaga aattgcttta aacataaaga gattaagaga tataaaatgt
    13621 tatagaggaa taagacacgc taaaggtctt ccattaagag gacaaagaac taagactaat
    13681 gctagaacta gaaaaggtcc tagaaaaaca gtatctcgta aaaagaaaaa ataaataaat
    13741 aataactaga tgaaggaggg aaaaggaaaa tggctaaacc aaaaaagaaa gttacacgta
    13801 ttagaagaag agaacgtaaa aatatagaac gtggtcatgc tcatatacaa tcaactttca
    13861 ataatacaat aataacttta actgacgttc acggaaatgc tatatcttgg gcaagttctg
    13921 gacaattagg attcaaagga tcaagaaaat caactccatt tgcatctcaa atggctgctg
    13981 aaacagctgc aaaagctgcg atggaacacg gactaaaaag tgttgaggta ttcgtaaagg
    14041 ggccaggttc aggaagagaa gctgcaataa gagctttaca agcaactgga ctagaagtaa
    14101 ctatgataaa agacgttact ccaatcccac ataacggatg cagaccacca aagagaagaa
    14161 gagtgtaatt aataagagta caataggagg tgtagataaa tggcaagata tacaggtgca
    14221 tcatgtagac aatgccgtag agagggaatg aaattattcc ttaaaggtga tagatgttat
    14281 acagacaaat gtgctatagt aaaaagaaac tatgctccag gtcaacatgg ccaaggaaga
    14341 aagaaagttt ctaactatgg attacaatta agagaaaaac aaaaagttaa aagaatatat
    14401 ggagttttag agactcaatt tagaaattta tatgaacgtg ctgaaaatat gcctggtaaa
    14461 gcaggtgaga acttattaag tttattagag agaagattag ataatgtagt ttacagaatg
    14521 ggactagcat cttctagaaa agaagctaga caattagtga ctcatggtca tttcacttta
    14581 aatggaaata aagtagacat accttcatta atagttaagg ttggagatgt tatagaagtt
    14641 aaagagaaat caagatcttc tgcaaaattc aaaaatttag tagaagttaa ctcaagaata
    14701 gctcctaagt ggttagaagc taatgtagaa ggaatgacag caaaagtagt tggtgttcca
    14761 acaagagaag atatagatct tgagatagca gagcacttaa tcatagaact ttactctaaa
    14821 taataagcac acttttaaaa attattttaa aattttgttt accctcagtg gattactgaa
    14881 ttttaaggag ggttttgtcc atgatagaaa tagaaaagcc aaaagtagat atagttgagc
    14941 ttagcgaaga ctatagatat ggtaagtttg ttatagagcc tctagaaaga ggatatggaa
    15001 taactatagg taacgcatta agaagaatat tattatcatc attaccaggt gtagcagtga
    15061 atgctataaa aatagatgga gttcttcatg aattttcaac aatacctggt gttaaagaag
    15121 atgttactga gataatatta actttaaaag agctttcagc aactatagat ggtgaaggaa
    15181 gtagaacact taaaatagaa gctcaaggac catgctctat cacaggtgca gatataatct
    15241 gtcctccaga tgttgaaata ttaagtaaag atttagcaat agctacatta gatgataatg
    15301 ctaaacttaa tatggagata tttgtagata aaggtagagg ttatgtttct gctgaagaaa
    15361 ataaaacaga gaatgttcca ataggtgttt tacctgtgga ttcaatatat actcctgttg
    15421 agaaggttag ttatcatgtg gaaaacacaa gagttggtca aaaaacagat tatgataaat
    15481 tagtattaga agtttggact aatggtagta taaatcctca agagggaata tcattagctg
    15541 caaaggtatt agttgagcat ttaaacttat ttatagactt aactgaacat gtaagtagtg
    15601 ttgaaatcat ggtagaaaaa gaagaagatc aaaaagaaaa agttcttgaa atgactatag
    15661 aagaattaga tttatcagtt agatcatata actgtttaaa gagagcggga attaatacag
    15721 ttgaagaatt agctaataaa tctgaggatg atatgatgaa ggttagaaac ctaggtaaga
    15781 agtcattaga agaggtaata caaaaactag aagaacttgg attgggatta aaaccaagtg
    15841 aagaatagcg agcaaaggag ggataagaat ggctaagtac cgtaaattag gacgtgaaac
    15901 agctcacaga aaccttatgt taagaaactt agtaacttgc ttactaagaa gtggaagaat
    15961 agaaactaca gtgactagag cgaaagaaac tcgtagaatg gctgaaaaga tgataactct
    16021 tgctaagaga ggagatcttc atgctagaag acaagtttta gcttatgtta tggatgaaac
    16081 agtagttaat aacttattca cagatttagc tccaaaatat gctgagagaa atggtggata
    16141 cactagaata ataaaaatag ggccaagaaa aggcgatgct gctgaaatgg cttttataga
    16201 attagtatag taaggaaagg attaggcttg atggcttaat ctttttttta tttttttgac
    16261 taaggtatga tataataatg tgtaaaatga tttttataag ttattgctta ctaaaaggag
    16321 tatgagttaa tggataatat agtaaaggta aataatattt cgtttgagta tattacagat
    16381 gaagcaaaac ttaaagcaat agataattta agcctagatg ttaaaaaagg agaatttgtt
    16441 gcgataatag gacataatgg ttctggtaag tctactttat ctaaaaactt aaatgctatt
    16501 cttatgccta ctgaaggaaa tattcttatt gatgatatgg atactaaaga agaagaaaga
    16561 ttatgggata taagacaaac tgcaggcatg gtatttcaaa atccagataa tcaaatagtt
    16621 gctactatag tagaagaaga cgttgcattt ggacctgaga acttaggtat agaaccaaaa
    16681 gaaataagac gaattgtaga ggagtcttta aaaagtgtag gtatgtatga tttaagagat
    16741 agacagcctc atttgttatc aggaggtcaa aagcaaagag ttgcaatagc aggaattatt
    16801 gctatgaggc ctaaatgtat aatatttgat gaagcaacag caatgttaga tccatctgga
    16861 agaaaagaag ttatgaaaac aataaaaaga cttaataagg aagaaaatat aactgttata
    16921 catataacgc actttatgga agaagcagta gaagctgata gagttgtagt tatggaaaaa
    16981 ggaaagaaaa tcttagaggg aactcctaga gaagtattca gtaaaataaa gatgttaaag
    17041 gaaatcggtt tagatgtacc ttgtatgaca gagttatcta gtttattaat agaagaaggt
    17101 ataaatatta gtagtgatat attaactgtg gatgagatgg tgatggaatt atgtcaatta
    17161 tagtaaagaa tttaacacat atatataatg aagggatgcc ttttgcaagt aaggcacttg
    17221 atgatgtttc ttttgagatt aaagatagag attttgttgg tcttataggt catacaggct
    17281 caggaaaatc tacattaata caacatttaa atggattact aaagccttcc tcaggagaaa
    17341 tatttataaa tgattttaat ataacagata aaaacttgaa tttaactgag attagaaaaa
    17401 gagtaggggt tgtatttcaa tatccagaat accaattgtt tgaagaaacg attgataaag
    17461 atatagcttt tggaccctct aatttaggct tagaggagtc tgagatacat aacagggtaa
    17521 aagcatcaat ggaagctgta ggacttgatt atgagggatt taaagacaaa tctccttttg
    17581 aattatctgg gggacaaaag cgtagagtag caattgctgg agttatagcc atgaacccag
    17641 aagttctcat attggatgaa ccaactgctg gtcttgaccc tggtggaaga gatgaaattt
    17701 ttaacttaat aaaagactta catgaaaaaa agaatatgac tataatacta tcatcacata
    17761 gtatggatga tatggcaaag ttagcaaaaa ctttaattgt tatgaatcat ggtagcgtag
    17821 aatttatggg aactcctaga gaagtattta aatcaaatgc tagtaaatta aaagatatag
    17881 gattagatat tccacaagtt ttagagcttg cattgaaatt gagagaaaaa ggctttgaca
    17941 tcagtgagga tatattgact ttagaagaag caaaacagga aatattaaaa gttgtgagag
    18001 gacgaggatt atgttaaaag atataactat agggcaatac tacccaacaa gttctgctat
    18061 tcataaatta gatccaagga taaagcttgt agcgacaatt gtatttatgg tatctatatt
    18121 tgtagtaaat aaattttggc cttatatagt agttttatta tgtttattag ctatgataaa
    18181 attggctaat ataccagtca aatatattgt taagggtgtt aaacctctta aatggattat
    18241 tttgtttaca tttcttataa atattttttt cttaccaggt gatgaaatat ggtcatttgg
    18301 atttttagct attacaaaac agggtctaag acaagcaatt tttatggcaa taagattaat
    18361 atttttagta gttggtacat cattgcttac tttaacaact tcaccaatag aattaactga
    18421 tggaatagag agattgttga atccatttaa gaaaattggt ttgccagttc atgaacttgc
    18481 aatgatgatg acaattgctt tacgttttat tcctacattg ttagatgaga cagacaaaat
    18541 aatgaaggct cagatgtcta gaggtgcaga ttttgaaagt aagaatctta taaatagagc
    18601 aaagaattta gtaccacttt tggtaccttt atttgtaagt gcatttagaa gagctgatga
    18661 attggctatg gccatggagg ccagatgtta cagaggtgga cataatagga ctaagatgag
    18721 agagtctgtt atatcaagaa gggattatat ggcatgtgta tttcaggttg tttatctggg
    18781 agcaattata gctactagat ttatagcaat ttaattaata agttatagat ataatgaggt
    18841 aaagtatgag aaatataaaa attaaaatcc aatacaatgg taagaattat tgtggatggc
    18901 aaaagcaacc tgattcttta gggatacaag gtacaattga aagggctata tatgatataa
    18961 caaaagaaga aaccagtctt atagggtctg gtagaactga ttcaggagtt catgctattg
    19021 gtcagattgc aaattttaaa attaattcag gtataagtat agaaagtata ccaatggcac
    19081 taaatgccaa gttgccaaaa gacatatcag ttatagaagc atgtgaagta aatgatgatt
    19141 ttcactccag atacagtgca aaaggtaaaa catataaata tttagtatat aatagcaagt
    19201 ttagaaatcc aatacttagt gagatatcat atcaagtaaa atatgaactt gattttgata
    19261 aaatgtgttc agaagcaaaa agtttattag gaacacatga ttttaaaggt tttatgagtt
    19321 ctggttcatc tgtaaaagat acagttagaa cgatatatga tattgatata agtaaaaaag
    19381 atgacttgat tacttttgag attagtggta atggtttttt atataacatg gtgaggatta
    19441 ttgttggaac acttgttgat atgggtcgtg gcagaattaa cgaaccattt ttagatataa
    19501 tacaatcaaa aactaggtca agatgtggac atacagctcc agcacaagga ttatttctca
    19561 aaaaagttca ttattaactt gacacaccag gctacatgta ttaaaatata tgagaatgtg
    19621 ttaataagtc cacttgcccc ggacttgaca taaacggtaa aaaaatttta tgaaaagtag
    19681 ttaaggaggg acagttatga aaagttatat agctaagcca gctgatgtac aaagaaaatg
    19741 gtacttagtt gatgctgaag gaaaaacatt aggtcgttta gcaacagaaa ttgcgacagt
    19801 attgagaggt aaacataaac caacatttac tcctcacgtt gatggtggag attttgttgt
    19861 tgtagtaaat gcagaaaaaa tagttttaag tggtaagaaa ttagatcaaa aatattacag
    19921 ataccatact ggttatgtag gtggattaaa agaaatatct tatagagata tgatggataa
    19981 gaaaccagaa gaagttatat cacatgctgt aagcggtatg ttacctaaaa ataaattaag
    20041 aagcagaatg atgactagat taagagtatt tgcaggtgct gagcacactc atgcagctca
    20101 aaatccagaa gttttaaact ttaaataaga ttaataggtg aaaggaggag tttataatgg
    20161 ctaacgttca atattatgga actggaagaa gaaaaagttc tgttgctaga gtaagactag
    20221 ttgcaggtga aggaaatata ttagtaaatg gaagagcatt agaaaattat tttaattatg
    20281 agacattaat aagagatgtt aaacaaccat tagttttaac tggtaatgaa aataaatatg
    20341 atgttatagt aaaagttgaa ggtggaggat tcactggaca agctggagct ataagacatg
    20401 gaatatctag agctttatta aaagctgatt tagatttaag acctgcttta aagaaagaag
    20461 gtttcttaac tagagatgca agaatgaagg aaagaaagaa atacggatta aaagcagcaa
    20521 gaagagctcc acaattctca aaaagataat tacttatttt tttggaaaag caaaatcctt
    20581 tatttactgt tattcagaaa gtattggtat tttaaaaatg ttgggttaag ttaattattg
    20641 atatataata aaataataag atatcttatt aggatgtttt tcattaagaa gaacatcctt
    20701 tttattttga ctatttaata aaaaacatat gctaataaca tttaaatttt atttttataa
    20761 tattgtaatt taaaagttta tataaggtta tatattttaa gatattattt aacaatataa
    20821 attctgaata tacgttatca aaatttataa tataaactaa gtataattta ttgtatagta
    20881 ttgaatgtta gataaacata gtgaattaaa tagggacaga aatataatat cattacataa
    20941 taatatatta ataccaataa ataaagatat atcaatatta aaaaaattta aatgtttgta
    21001 ctaatatatg gaatattata gtgtttttat aagtatacat atacagggtg atgttgtgag
    21061 aaagtacata aaacatataa tttttagttt tgcaatgata tgtctagtag tagtatccat
    21121 atttgaaatt aaaaatattt ctgaagatgt tatcaagtat atgccagtaa caaacaaaac
    21181 tataatttta gatgctggtc atggaggcat tgatccaggt gcattaaata aggataagag
    21241 tacatctgaa aaggatatta acctagcaat aacacttaag cttagagagc ttatagaatc
    21301 aagtggtggc cttgtaatat taactcgaga agatgacagt agcctttata aagaagaaaa
    21361 taataaaact acaagacaaa aatataatga gaatttgaaa aatagaaaag aaataatatc
    21421 taattctaat gccaatatgt ttgtctctat acatcttaat gcatttgaac aatcaaaata
    21481 ctatggagct caaacttttt atcctaaaga caaacaagac agtaaagaat tatcgaagtg
    21541 tattcaagaa gaacttaaaa gagtagtaga taagacaaac aacagagaag tcaaaccaag
    21601 ggatgatata tatcttttga aagaaaataa tattccatca gtactgatag aatgtggttt
    21661 tttatcaaat gaaaaagagt gtaaactctt aactgatgaa acatatcaag aaaaaatagc
    21721 atgggcaatc tacataggaa tacaaaaata tttaagttga aataactata aaaataggtc
    21781 taaatatgta aatagcttgt attaaagcag ttaaaatgca ttaatatagg ctatttttat
    21841 tttgacaaaa aaatatttaa aataaaagtt aaaaagttgt tgacttagaa taatatagat
    21901 gatattatat atgagtgccc aaaaggagca ccaaaataag ac
//
LOCUS       DAMJYR010000056        21660 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.056, whole genome shotgun sequence.
ACCESSION   DAMJYR010000056 DAMJYR010000000
VERSION     DAMJYR010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 21660)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21660)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21660
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.056"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(263..1201)
                     /locus_tag="PC471_002124"
     CDS             complement(263..1201)
                     /locus_tag="PC471_002124"
                     /inference="COORDINATES: protein
                     motif:HMM:NF012798.1,HMM:NF024886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDF2789252.1"
                     /translation="MATFEKRSKRIKIEGNINKNNEKLIKGFLQAKKIAGLSEKTIYV
                     YKCDLYTWAKYLHDEMEDLLIEDCTEDDIMEYISFRMEDNHVNRIKVIFSGISSLYDH
                     LRRKRIVTENPVSLIERPKKGLPVVEKHFLNIEQAKELQDKLSKQDDLQLEVYINFAL
                     STAGRVTATSNLRWDNIDFDNRVCENIKEKGGKIVDFYFSEKVKDLLLKLKEVRKKED
                     IVNDYVFLVKYDKEYHKATSNVLRSWAKKAGKLIDIDNLAPHSLRRSFATIAKANNLP
                     LEDISTILNHESTDTTKIYIKEDKGKISKSKDMIGL"
     gene            complement(1236..1547)
                     /locus_tag="PC471_002125"
     CDS             complement(1236..1547)
                     /locus_tag="PC471_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2789253.1"
                     /translation="MLSKEMVELLSQYGYTAILLMVLMLWLGKYLEKNRQLEQDDRKK
                     ERAYFSREIKEQRTLFGNTIDKFDDKLDKFAEALNTNNSRLERVETDITKIKDKLETR
                     D"
     gene            complement(1548..1799)
                     /locus_tag="PC471_002126"
     CDS             complement(1548..1799)
                     /locus_tag="PC471_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HDF2789254.1"
                     /translation="MDIMQFIPENLIMLIGGLYILGTFIKESNIKNKYIPFILLVIAM
                     ISSCLFMKELSIEAVFEGILCWGASIGINQIQVQNRKEK"
     gene            complement(1811..2428)
                     /locus_tag="PC471_002127"
     CDS             complement(1811..2428)
                     /locus_tag="PC471_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HDF2789255.1"
                     /translation="MNILDKSTATLEQVFSYLDTLKNNSNPPHFLCRAIVPIIYKEAE
                     KKGVNPVIAIAQAFVETGYFNFGRVLNPSYCNVCGLKGNKGGGDLDPTAHTRFNFWED
                     GVSAFIDHLALYAGAKGYPKYSELVGKVEYKVNGTTLDPRHFPYLHGEAKTVESLSGK
                     WCPDMNYGQNIINICNKISSMKVENNDVKLEQIKTKVKELNEILG"
     gene            complement(2440..3255)
                     /locus_tag="PC471_002128"
     CDS             complement(2440..3255)
                     /locus_tag="PC471_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HDF2789256.1"
                     /translation="MNIKTLTIHAGHNPDNKIGSGAIGNIKESTEARNVLKELLPLAQ
                     KECKVYDCTCNNGTSQSDILNKIIAKCNSYNTDLNVSIHFNSGGGRGVEVLVYNLNDK
                     ETVEIASRICKKITETYHAKGDKDFKNRGVKEKKTLAFLRRTKAKSILVECCFVDTSD
                     TKKYNAKDMAIDIYEGIFNKSVAGKPQDNKVKYAIVYEGDVDKVIAQLMAMNYKTNEV
                     SVCDLKNYVPGHCENLYVIGGASSKIKTSERFTKLQGDDRWATLHKVLDFIGK"
     gene            complement(3336..3506)
                     /locus_tag="PC471_002129"
     CDS             complement(3336..3506)
                     /locus_tag="PC471_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2789257.1"
                     /translation="MYNILKRMIEQKNYETREELQTKLDVFYAMNRIKESEYTELTNL
                     LNKEDTLVEPII"
     gene            complement(3532..3852)
                     /locus_tag="PC471_002130"
     CDS             complement(3532..3852)
                     /locus_tag="PC471_002130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2789258.1"
                     /translation="MDIEKKIEILIEYGFENDLNNIDNYFIAEGKKIFTPVIKNGELI
                     KTGEQVYNEWIELQNNPPKPSIEEINADKITILIENQKQQDSLLVDNAYRIAMLELNT
                     NNVL"
     gene            complement(3867..5768)
                     /locus_tag="PC471_002131"
     CDS             complement(3867..5768)
                     /locus_tag="PC471_002131"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2789259.1"
                     /translation="MIYKDLLKKVNDIENLKEVDNIKCSTDTGEYTIENSKKGYLTNF
                     NVEGKTLIDLWGKTSSDFSLWKATFVDGKINILTENDIRYSNFFTINYTSYKPDTIYT
                     IIVDVDKNTLPSTSGIYIHSLGEENSVFIPNLTNIAIPGGVIGKFKYTFTTISDLNDC
                     NVVLRSVLDNDTLTSGYEISLKITILEGDYTDINIDYSNELLSVGQRDKIEFLSYQYS
                     GINIFNKNADFKDNYILQYLSGEELISEASNHKYTLDYIEIEPDTEYTFYNCSRNICW
                     YDINKNFIPASLNERIIGDKDIFYIARSPKNAKYLRVTIIKDLHDNGNKTIITKGNKY
                     DKKTIPYTLRSLPNGVKDEIVYKNDKYYLIKRCEEHTYTDIGNLNLSHVYDNTLQFMG
                     TLTPQAVVDSLDTAYVLCNNLNGKSRNDFNNNDIEGCSTTGSGDIAFKILKSKLTTQD
                     GNGFSEWIKNNPITIIYQLSKPQEIELTSLNLEQYNNQTKFICNTGIVIPDISFESTQ
                     NLGSHIEVIRNNIKNYNVRTDFPFSINFLNGWQPYLGYASSVGNYCTNNNLVTINATI
                     NGGITTAGTIIGKIPSKYAPRKGIIVIFQTTDGKYYNGIIRTNGEIEIYYNDITYRSW
                     LYLYVNYLI"
     gene            complement(5796..5978)
                     /locus_tag="PC471_002132"
     CDS             complement(5796..5978)
                     /locus_tag="PC471_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015981517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2789260.1"
                     /translation="MNINNVVVRILAERILSRGLNPLKNRPFELDDVTNEDYRKAVED
                     CIIKESGVVERVETTK"
     gene            complement(5978..6271)
                     /locus_tag="PC471_002133"
     CDS             complement(5978..6271)
                     /locus_tag="PC471_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2789261.1"
                     /translation="MNRANRIIYDQTGKILLQTGEATGDILEHDTITELHYIDVEYGN
                     IDYSKQYIESINPITKEPILKDIPIYLSEEEKRIQELENQILLNENEKVGGLL"
     gene            complement(6285..8000)
                     /locus_tag="PC471_002134"
     CDS             complement(6285..8000)
                     /locus_tag="PC471_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2789262.1"
                     /translation="MQTEWNFGYNGSPQSVILKPGKYKFECWGSSGGINNSSWHTDAK
                     GGYSKGEITLKKQTTLYVYVGESGFASSSTSNNTKSGFNGGGKGYLNQQVMGTYYSMY
                     GGGATDIRLVGGAWDNEQGLLSRIIVAGGGGGSYSPYTGGAGGGLAGGTGYSANDRHR
                     PGGTQYQGGIGRVSTENGSFGKGCSAKDSTGEGGGGGWFGGAGMNGVGAGGGGSGYVL
                     TKDSYKPTGYTPTSEYYFDNVVMESGGNTAGAYGYAKITLLQALPFLTVSSYNSITAT
                     FKADHTDPTLLTKIEYFIDDILKETITTDLTTEKTINYTLEDNALHTLKIVVTDSNNT
                     TAEKVLSISKNIMPLPENVNLNDISTKLVEVNAGFKVGKTSIINTLALKNIEASLNNT
                     LVELSEKIKTSFDSSDASVQDLMNQLTQANNTISQLNSKYKYATGTAYARENSSLIAC
                     VYDPNTSHTVTETSPYWLDLNGIGFIPDIFFAECEYEPHSNAFYKYFVFAIKNTFPIS
                     NNTGFVVNIAFNKEYGDESFKLRGALYTLGKRHVSMDNNGVRVPSLSTLNDFRAYKWH
                     AIKFK"
     gene            complement(8017..8556)
                     /locus_tag="PC471_002135"
     CDS             complement(8017..8556)
                     /locus_tag="PC471_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2789263.1"
                     /translation="MLNEDVVKKLKNVPNNTNTEIEKVNTNIETAKTELNTKIDQLIA
                     GGSNVASTQTITIDDWVDDTENGFKATVTHGLLTQRIVVNIIDATTKENVVTNFKIID
                     DNSIEIRSETRSELNVYVINGNAETHFINATVDDNRVSEMTTYSSKKIEDRFVNLEEK
                     VNGGLSNIATSVNELITYC"
     gene            complement(8636..8959)
                     /locus_tag="PC471_002136"
     CDS             complement(8636..8959)
                     /locus_tag="PC471_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2789264.1"
                     /translation="MIKIDKEKIEKSRQRKELDDLDSSQKTQDNDITDLMLATAEICE
                     MVLSSQQTSTMSLKNIKLNEGGSSMAAIYVGLIERGLKTIDQVPVKYREEVRKMCEAL
                     EISLS"
     gene            complement(8971..14250)
                     /locus_tag="PC471_002137"
     CDS             complement(8971..14250)
                     /locus_tag="PC471_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2789265.1"
                     /translation="MKKNKLLQRGSLFGNKEFKIEEFNKNFDTFNVKDIFTDMDSGSV
                     FIIKDDLKLYAAGYNVFGNLGLGNKVSPIRYFSKIDIDNVKYVSSSGTHSLLLKNNGD
                     VYSTGLNTDGELGLGDNIDRNTFTKINISNVKQIACGNGYSMLVTNDNELYVCGNNKF
                     GGIGLGNTSTVNTFTKIDNLSVKEIFAGVSCSYILTLNNEVYSTGTNSYGQLGLNDTT
                     NRNIFTKVNLDNVIKISTAQGAVNFLLENGDVYGCGDNSSGQVGVGGGATVTVLTKLS
                     ISNVKNIESGRYHKFYIANNNDIYVTGANPDGRLGDKINCDTSTYTPRKSSISIKNIS
                     KIKCAYAISFILTSNNELYMTGNNNYSFSYLKRYDKFIYGFEKFEDFDIEECNHIESI
                     DNRLLINKNDERVLDIGLENNFVDISLVKANDTSTFIVVNGNELYAAGYNAYGGLGLG
                     HNRHCYEFTRVPLELEEGVTIKDIYVNIGSYFTFILLSDNTLYSTGYNVFGVLGLGDN
                     INRNTFTKVNISSVDKISVGDNHALLKTTTNELFSTGLNNYGQLGLNDNTNRNIFTKV
                     STVTYVNEIIAYSNSSYCIDNSRISYAAGYNVFGELSLNDTSSRSVFVKMKYLRYKTD
                     TTVSDVSNIAEIIPFNNGCLIRTTSNNYYVSGRHNYYSTSIKNSVTDDYYSRFGEFVG
                     SKNSNANEVAVLIDEPKLSKVLVSNYNLCLLLDASDGGYKEIKFTGRPSTFGASSQDK
                     NLGLRSLISSSMPYYNHYLGTDNLLYTTSKTRMESKFEIRGRNAGGQRGDSSYYSNSQ
                     KYTLSNAFFAYNSQVIYASRLRIVIKDYFGNIYCSGVNSHGCFGIKKPYDVRHDGFAN
                     ISKQFSKKYIISNIKEIKSDRQALYILLNNSEGYVCGNNAGNKLGTIKARSEFIKVPI
                     DNIKEFIPSNDCLFILTNNNQVYSIGTNDLGQLGLGDNIDRGTFTKVPIDNVKKVITC
                     YKSSYILTYDNELFSTGSNGYGQLGLGDNINRNTFTKVNISGTIKDIFCGRYVFIILL
                     ENDNIISLMGCGNRNYISSNTNSNSLTKINIEGLTDPSTISNLMLEEGKTIITMKNRI
                     FVTGYNSLGGLGVGTSYNNSSGYIEGYKDLAFNSNIKEVALGEYTIFVLLENGDLYSG
                     GNADDGIGFNESGTVVLKKLTSNIKHIYANGKNAYYIDNDNALYVFGSRQLGQINNYS
                     DVVQKTPVRNCLNVKQVYTQHNGLIVQYTNNKLEGKGNAVVGQVGIFNGHEENQIANT
                     SGDNPSDLSFSNEMSIIRNNYGLLVNKKIFLHNDDSINYRIPFGTNAGNYSTSLPFDN
                     IKKMSLSSTHSILILENGDVYGCGSNQFGELAINKSELTTTSEFIKINLTNIIDIACG
                     DNFTYFLKNDGSLFSIGKNSEYQLGIGHNDEVNELQEVLTISNIREIYAYSNYILAIT
                     TEGQLYVQGYNINGILGLNENTKNTIIKLFTKVLENVKYIKSYDDKHIFVIKNDKTLY
                     VTGINKETYKIKDVEIGSMLYTFSQIYIPRDVNDIVDALIKDETLYIISKVDSEKTCL
                     EIKNKSYSSIEIDLQNPNNELTKIEMFINNISSSIIEDLSTGTVTFEINPENLVLGEN
                     KIVFKAHSDIGNNLYINVYIYKKETGATIVKDSTVLIDGNTYNVSSIVDNSQDVVLTL
                     NKGLLEDLNSNNPIYHLVNKLKVQLKINESDTFKDMVKVETRKTENGYKEIYELKDMN
                     IQSAQPKVIVEEGNTNTTIKKPSMLFNLDVETL"
     gene            complement(14261..16339)
                     /locus_tag="PC471_002138"
     CDS             complement(14261..16339)
                     /locus_tag="PC471_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDF2789266.1"
                     /translation="MKNYEIKNHTIEVDFSNYRINKRLLDYFIYNENDVKTAYIEAIL
                     KNKDEIIDLSEYDRVLVSITKSDGQKVNGECEVVDAENGVVEIELSRQALASVGINTF
                     QLSLVKDGTLLNTTNLYYRVEEGMINDDDITSTDEYGVLLVIISQAEEIIKNNKELTK
                     RVEQLEITILGNEEVRDKAEQIRIYNEDIRNIQEEEREFNELTRQNQESNREESIQNM
                     QIQVDDKLTDCQLQLDEMIDAKSEEIDNIVDDKMLDVQAQTDKKFQDLDTRANNTFDL
                     YDKTFEDKLTDNQEQIDYKLDEVNQAISNVEDCIDESTTKLDTKIKEVDDKIVEVNTA
                     KTDIEATVANKMVELDDKFDSLASLDARGELIQARESVDGTVKDTLKDRLTYDFEKVN
                     EKIAEMTSAATNVAFSKSYVESDWVADGEYFKLIVNHNLVTENIFVAILDEATKKSMT
                     NSYTIVDSNTIEIFNESNIDVKVTVVNGNTNKEVIQATINDNITTLDSTYSSIKIDAK
                     FDESLTKINENKSNIATNLEKINLIQSKVGSSELSTVSKNISDAVNELDASVKVLESG
                     GNTNEQLKILKENYNKMSVEILKILFELELDKEATADEAGYWYDTLYDSKNISNLDGL
                     KLNKFKQQLELLSNSGTVLFKNVTVPFLCNKIRYIHDLGSNYSETKTEESYQKGDTEI
                     KINKYSYEVR"
     gene            complement(16351..16506)
                     /locus_tag="PC471_002139"
     CDS             complement(16351..16506)
                     /locus_tag="PC471_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2789267.1"
                     /translation="MEVNLQKAYTVAFEEIKSLYNELILYKALNMQQQEEIENLKKEL
                     EEQNKEQ"
     gene            complement(16508..18430)
                     /locus_tag="PC471_002140"
     CDS             complement(16508..18430)
                     /locus_tag="PC471_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber domain-containing protein"
                     /protein_id="HDF2789268.1"
                     /translation="MILDNSPADSWIRITGVIVTYNNTLYQVVDKETNKKYIYWDADN
                     PGTLKVSNVRLPEGNTQFLVVVNDNGKHTEVPTNSSIFNISFDGNSRKNTEEQIWALY
                     ETDEKHNEKFVVIEKDIDGIHQTVLEVQEDASHIKENMSLIDQRTENVNILVKEVTKN
                     FGGSQENITLRENINKAIIKLNTDLGTFSSNISNYFNDNEVTDEEKEKIDIELNLLDT
                     DKASLYTELQKLIDRTTGVDLVAINTSKTALDAASTNLNSIINSVISDSIITPSDRIL
                     AINANAQYNLKINELKNTVDRIYITGMGGSISEEFSQINATAKEIKLEVAKVDGATKT
                     NAAEIKLTKDDITMMVTRNNSGSIVGIKPDKIEFGFNDISNYVEISRSGLTVNQGAIA
                     CDILTTPSGHEPIIRLFGSSRSGFAIDARQSDGSSQASAIRLKYDSNNYFWVGYDTAE
                     IYVDGEEHFIVERDDTFVRCGGATFTFTNGDSLGVGYSFYPERSVTDLGCERYKWRYL
                     YARSTLSESDKKFKENIVYIKDIKNRTRSSITPTPFLDFIRDDFKPATFDYIAEKDRT
                     IADSQIGFIANDFKDSYVGKTFLYDYGEENGLMFSPSGYTTVVATALQEEIQKREELE
                     MIINELNEKINNLGGY"
     gene            complement(18432..20147)
                     /locus_tag="PC471_002141"
     CDS             complement(18432..20147)
                     /locus_tag="PC471_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2789269.1"
                     /translation="MHFNVNLKQIKSDYTLTIHKMNKSFLGQIPINFLNSIKRELGGV
                     DEIQLTIPKYITDRFLFNKIINPIFEEVKEERLICLNNKEYFVVKNVVTTDDKLKVVT
                     AKSKEVKLGKIDVNIEDYGLQMFTKDEEASIISLNDYLKQETGWKLGHVDDSIAYETD
                     SEGNKREKVRWQESINSNWLDYFNNELKEQFECIADFDTYNNLVNLYHIDSFGDNIQL
                     YLSHDNYIKSLERTTNSDDIVTRLKLEGSEDMNVIGATVTGYDYIENYSYFLDNKEMS
                     EELSKAIKKYQEMNEIREPIWRELIDTKLKKQRERDSKSNEWQMVIEMISKKKDIKKT
                     YDNPQHKDEVNSAKIAVEISELEDKKVILDVQIKHLEEEIAKLNESIKDINILCKRET
                     STDEDGYLIFNEVLLDELNEFLYYDTYTNDAFLKVEDLIAEGKRQLSLKCIPTREWTL
                     DVINFLDRIIDINFRQHWKGDLSLGDIIVLYSKESKEEELVYFTSFTQNLKNGKLDTL
                     ELTLSNKKIKEDDKRTIADYLTKAEHATRTLNSKRHLFIQQQKKRINLPDEYIPKKNI
                     QKELM"
     gene            complement(20479..20634)
                     /locus_tag="PC471_002142"
     CDS             complement(20479..20634)
                     /locus_tag="PC471_002142"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2789270.1"
                     /translation="MINNIEDCKLLIETEIRMKGIDEKELISQNEILKDLNINEKDLE
                     DIEVNFE"
     gene            20894..21055
                     /locus_tag="PC471_002143"
     CDS             20894..21055
                     /locus_tag="PC471_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009900785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2789271.1"
                     /translation="MEYLVLFLIMIIMITRIINHLTKLIDAILNLKASIRKLKNHQTG
                     GSSDSESNT"
BASE COUNT         6807 a         3727 c         2261 g         8865 t
ORIGIN      
        1 tacttatata acatataata ttattaaata tatgttatat aagtatgtat atatttaata
       61 agaagctatt gtagacaaaa aaataaagcc ctttaaagaa aactcattta agagtaatcc
      121 ttaaagggct tattatttat ctatctcttt ttcttacctt ttttacgact tttacatgcc
      181 atgatatcac ttcttttcta ctttatatct atttataaac ctatcatatc tttagactta
      241 gaaattttgc ctttatatct atttataaac ctatcatatc tttagactta gaaattttgc
      301 ctttgtcttc ttttatataa atcttagttg tatcagtgga ttcgtgattg aggatagttg
      361 atatatcttc aagtggtaaa ttattcgctt tagctatagt tgcaaaactt cttcttaatg
      421 aatggggagc taagttatct atatctatta atttaccagc tttcttagcc caacttctta
      481 atacattaga tgtagcctta tgatattctt tatcatattt aactaagaaa acataatcat
      541 ttactatatc ttcttttttt ctaacttctt taagctttag taataaatct tttactttct
      601 cactaaaata aaaatctact atttttccac ctttttcttt aatattttca catactctat
      661 tatcaaagtc tatattatcc catcttaaat tacttgtggc tgtaactcta ccagcagttg
      721 atagagcaaa atttatataa acctctaatt gtaaatcatc ttgttttgat aacttatctt
      781 gtagctcttt agcctgttct atattcaaga aatgtttttc cacaacaggt aaaccctttt
      841 taggtctttc gattagagat actggattct ctgtaacaat tctcttcctt ctaagatggt
      901 catatagaga tgaaatgcct gaaaatatta cttttattct attaacatga ttgtcctcca
      961 tcctaaatga aatatattcc attatatcgt cttcagtaca atcttcgatt aataaatcct
     1021 ccatttcatc atgcagatat ttagcccaag tatataagtc acatttatat acatatatag
     1081 ttttttcact taaaccagct attttcttag cttgaagaaa tcctttaatt aacttttcat
     1141 tatttttatt tatgttgcct tctatcttaa ttcttttact tcttttttca aatgttgcca
     1201 aatacttcac ttccttataa aaaagactag agaaattaat ctctagtctc taatttatct
     1261 ttaatttttg ttatgtctgt ttcaactctt tcaagtctac tgttattagt atttaatgct
     1321 tcagcaaatt tatctaattt atcatcaaac ttatctattg tattaccaaa taaagttctc
     1381 tgctctttaa tttctcttga aaaataagct ctttcctttt ttctatcatc ttgttctagt
     1441 tgtctatttt tctctaaata ttttccaagc cataacatca ataccattaa aagtatggct
     1501 gtatacccat actgacttaa taattcaacc atttctttag ataacatcta cttttccttt
     1561 ctgttctgta cttgtatttg atttattcct atagatgccc cccaacataa aataccttca
     1621 aaaacagctt ctattgacag ctctttcata aacaaacaac tacttatcat tgctataact
     1681 aacaatataa aaggtatgta tttattttta atattagatt cttttataaa tgttcctaat
     1741 atgtaaagcc cacctattaa cattattaaa ttttcaggta taaattgcat tatatccatg
     1801 tgatttcctc ctatccaaga atctcattta attcttttac tttagttttt atttgctcta
     1861 attttacatc attgttttct accttcattg aacttatctt attacatata tttattatgt
     1921 tttggccata attcatatct ggacaccatt tgcctgataa actttctact gttttagctt
     1981 ctccatgtaa atatggaaaa tgtctagggt ctaatgtagt tccatttact ttatattcaa
     2041 ctttaccaac taattcacta tattttggat atcccttagc tccagcatat aatgctagat
     2101 ggtcaataaa tgctgatact ccatcctccc aaaaattaaa tcttgtatga gctgttgggt
     2161 ctaaatctcc tccaccttta tttcctttaa gaccacacac attgcaataa gaaggattta
     2221 aaactctacc aaaattaaaa tatccagttt ctacaaatgc ttgagcaatt gcaataactg
     2281 gatttacacc ttttttctct gcttctttat atataattgg tactatagct ctgcataaaa
     2341 aatgtggtgg attagagtta ttttttaatg tgtctaaata cgaaaagact tgttctaatg
     2401 tagctgttga tttatctaat atattcaatt cattacctcc tacttgccta taaaatctaa
     2461 cactttatga agtgtagccc atctatcatc gccctgtaac ttagtgaatc tctcgctagt
     2521 tttaatttta ctggatgctc caccaatcac atataggttt tcacaatgtc caggaacata
     2581 atttttcaaa tcacacacag agacttcatt agtcttataa ttcatggcca ttaattgggc
     2641 tataacttta tctacatcac cctcataaac tattgcatat ttcactttgt tatcttgtgg
     2701 tttaccagct acagacttat taaatattcc ttcataaata tctatagcca tatctttagc
     2761 attatatttt ttagtgtcag atgtgtctac aaaacagcac tcaactaaaa ttgactttgc
     2821 cttcgttctt cttaaaaatg ctagagtttt cttttcttta actccacgat ttttaaaatc
     2881 tttatcacct tttgcatgat aagtttcagt tattttttta catattcttg atgctatttc
     2941 aacagtttct ttgtcattta aattataaac taaaacttct actcctcgac caccaccact
     3001 gttgaaatgg atacttacat ttagatcagt attatatgaa ttacatttag ctataatttt
     3061 attcaatata tcactttgag aagttccatt gttacaagta cagtcataaa ctttacattc
     3121 cttttgagct aaaggtaata actcttttag tacatttctt gcttctgtag attcctttat
     3181 atttcctatt gctccagaac ctatcttatt atctggatta tgtcctgcat gaattgttaa
     3241 tgtttttata ttcaaaatta ccactccttt taattaaaag agaattgaaa ttaatcaatc
     3301 ctctaaaatt gacataaaaa aagaaccttt atactttaga taataggttc tactagtgta
     3361 tcttctttat ttaataaatt tgttaactct gtgtattcac tttcctttat cctgttcata
     3421 gcgtaaaata catctaactt agtctgcaat tcttctctag tttcataatt cttttgttca
     3481 atcattcttt ttaaaatatt atacatgttg tttcctccat tttttatagt tttataacac
     3541 gttatttgtg ttaagttcta acatagcaat tctataagca ttatctacta ataaactgtc
     3601 ttgttgcttt tgattttcta ttaaaattgt aattttatcc gcatttattt cctctatgct
     3661 tggtttaggt ggattatttt gtaattctat ccactcatta taaacctgtt ctcctgtttt
     3721 aattagctct ccgtttttta tcactggagt aaatattttt ttaccctctg caataaagta
     3781 attatctata ttatttaaat cattctcaaa tccatattct attaaaattt ctattttctt
     3841 ttctatatcc atcttatttt tcctttctaa attaaatagt ttacatataa atacaaccaa
     3901 ctacgataag ttatatcatt ataatatatc tcaatttctc catttgtacg gatgattccg
     3961 ttataatact ttccatctgt tgtttgaaat attactatta tgcctttacg aggtgcatat
     4021 tttgaaggta tctttccaat gatagtacct gccgttgtaa tacctccatt aatagttgca
     4081 ttaattgtta caaggttatt atttgtgcaa tagtttccaa cagaactagc atatccaaga
     4141 tatggctgcc acccattaag aaagtttatt gaaaatggaa aatcagttct aacattgtaa
     4201 ttttttatat tatttctgat aacttctata tgacttccta aattttgtgt actttcaaaa
     4261 ctaatgtctg gtatcacaat acctgtgttg caaataaatt tagtttgatt attatattgt
     4321 tctaaattta gtgatgttaa ttcaatttct tgtggttttg aaagttgata gatgatagtt
     4381 attggattat tttttatcca ttcactaaac ccatttccat cttgtgtagt aagtttactt
     4441 tttaatattt taaatgctat atcaccacta ccagtagttg agcatccctc tatgtcatta
     4501 ttgttaaaat catttctact tttcccattt aaattattac ataaaacata tgctgtgtct
     4561 aaactatcta caacagcttg aggagttaat gttcccataa attgcagagt attatcatat
     4621 acatgtgata aattaagatt tccaatatca gtataagtat gttcctcaca tctttttatt
     4681 aagtaatatt tatcattttt ataaactatt tcatctttta ctccatttgg taaacttctt
     4741 aatgtatatg gaattgtctt tttatcatat ttatttcctt tagttataat tgttttattc
     4801 ccattatcat gtaaatcttt tattatagta actcttagat actttgcatt tttagggctt
     4861 ctagcaatat aaaaaatatc tttatctcct attattcttt cgtttaatga tgctggtata
     4921 aaatttttat ttatatcata ccaacaaata tttctactgc aattataaaa agtatactct
     4981 gtatctggtt ctatttctat atagtctagg gtatatttat gattactagc ttcagaaata
     5041 agttcctctc cactaagata ttgtaaaata taattatctt taaaatcggc attcttatta
     5101 aagatattaa ttccactata ttgataactt aaaaattcta ttttatctct ttgcccaaca
     5161 cttaatagtt cattagagta atctatatta atatctgtat aatctccttc taatatggta
     5221 attttcaaac ttatttcata accagatgta agtgtatcgt tatctaaaac actccttaaa
     5281 acaacattac aatcatttaa gtctgatatt gttgtaaatg tatatttaaa tttaccaatt
     5341 actcctcctg gtatagctat atttgtcaga ttaggaataa atactgaatt ttcttcgcct
     5401 aagctatgaa tatatattcc acttgtactc ggtaaagtgt ttttatcaac atcaactatg
     5461 attgtatata tggtatctgg tttatatgaa gtgtaattga ttgtaaaaaa attagaatat
     5521 cttatatcat tttctgttag tatatttatt tttccatcca caaaagttgc tttccataaa
     5581 gaaaaatctg aactagtttt tccccataaa tcaattaatg tctttccttc tacattaaaa
     5641 ttagttaaat agcccttttt actattttct atagtatatt ctcctgtatc agtactacat
     5701 tttatattgt caacctcctt gagattttct atatcattga cctttttaag taaatcttta
     5761 tatatcaaat taaaacacct ctcttcacaa aacccctatt ttgtagtttc tactctttct
     5821 actactccac tttctttaat tatacaatct tctactgctt ttctataatc ctcgttagtt
     5881 acatcatcta attcaaaagg tctatttttt aaaggattca atcctctact taaaatcctc
     5941 tctgctaata ttcttactac aacattattt atattcatta taatagccct cctacttttt
     6001 cattttcatt tagtaatatt tgattttcta actcttgtat tctcttttct tcttcgctta
     6061 aatagattgg aatatctttt aaaataggtt cttttgttat tggatttata gattctatat
     6121 actgtttact atagtctata tttccatatt caacatcaat ataatgtaat tctgttattg
     6181 tatcatgctc taatatatct cctgttgctt ctcctgtttg gagtaatatt ttaccagttt
     6241 ggtcgtaaat tattctattt gctctattca ttttatcacc tcatttattt aaattttatc
     6301 gcatgccatt tatatgctct aaaatcattt agagtgctta gtgaaggtac tctaactcca
     6361 ttattatcca tggaaacatg tcttttgcca agggtatata aagctcctcg taatttaaaa
     6421 gattcatcac catattcctt gttaaaagca atattaacta caaacccagt attattagaa
     6481 atgggaaaag tatttttaat tgcaaaaaca aaatacttat aaaaagcatt cgaatgtggt
     6541 tcatattcac attcagcaaa aaatatatca ggaataaatc caattccatt taaatcaagc
     6601 caataagggc tcgtttctgt aacagtatga gaagtattag ggtcatatac acatgcaatt
     6661 aaggatgaat tttctctagc ataagcagtt cctgttgcat atttatactt agaatttaac
     6721 tgtgatatag tattattagc ttgtgttaac tggttcatca aatcctgcac actagcgtct
     6781 gaactatcaa aacttgtttt tattttctct gacaactcaa caagtgtatt atttaaactt
     6841 gcttctatat tctttaatgc taaagtattt ataatacttg ttttcccaac tttaaatcct
     6901 gcattaacct caactaattt tgttgatata tcatttaaat ttacattttc gggtagtggc
     6961 attatattct tacttatact taatactttt tctgctgtag tattattact gtctgtaaca
     7021 actattttaa gtgtgtgtag tgcattatct tctaatgtgt agttaattgt tttctctgtt
     7081 gttaaatctg ttgttatagt ttcttttagt atatcatcta taaagtattc tattttagtt
     7141 aatagtgtag ggtctgtgtg gtcagcttta aatgtagctg taatggaatt ataagaagat
     7201 actgttaaaa atggtaatgc ttgtagtaat gttattttag cataaccata agcaccagca
     7261 gtatttccac cagattccat aacaacatta tcaaaataat attcagatgt tggtgtgtag
     7321 ccagtaggct tataactatc tttagttaat acgtagccac ttccacctcc acctgctccc
     7381 acaccattca ttcctgcacc accaaaccag ccacctccac cgccttcgcc agttgaatct
     7441 ttagcagaac acccttttcc aaaacttccg ttttctgtgc ttacacgacc aataccacct
     7501 tgatattgag taccgccggg acgatgtctg tcgttagcac tatacccagt acctcctgct
     7561 aatcctcctc ctgcaccacc agtataagga gaatatgaac caccgccacc acctgcgaca
     7621 attatacgag atagcaaacc ttgctcatta tcccaagcac caccaacgag ccttatatca
     7681 gtagcaccac caccgtacat agaataataa gtacccataa cctgttgatt taaataacct
     7741 ttaccaccgc cattaaaacc acttttagtg ttattactcg tagatgaaga agcaaaacca
     7801 ctttcgccga cgtaaacata taatgtagtt tgttttttta atgtaatttc acctttagaa
     7861 tatccgcctt tagcatcagt atgccaagaa gaattgttga tacctccaga agaaccccag
     7921 cattcaaatt tatatttgcc aggtttcaat ataacacttt gtggcgaacc attataacca
     7981 aaattccatt cagtctgcat tttctcactc tcctctctaa caataagtta ttaactcatt
     8041 tacactagtt gcaatattag ataaaccacc atttaccttt tcttctagat taacaaatct
     8101 atcttcgatt ttcttagacg aataagtagt catttcagat actctgttat catctacagt
     8161 tgcattaata aaatgagttt ctgcatttcc atttatcaca taaacgttta attctgacct
     8221 tgtttcactt ctaatttcta tagaattatc atctataatt ttaaaatttg taactacatt
     8281 ttcttttgta gtagcatcta taatatttac aactattctc tgtgttaaca aaccatgtgt
     8341 tacagttgct ttaaatccat tttctgtatc atccacccaa tcatcaattg ttattgtttg
     8401 agtagatgcc acatttgaac cacctgcgat aagttggtca attttagtat tcaactctgt
     8461 tttagcagtt tctatatttg tattaacttt ttctatttct gtattagtgt tattaggtac
     8521 attttttaat ttttttacta catcttcatt taacaattta tcaccttctt ttttatttag
     8581 agagctacat tagtaactct ctattttgaa cataaaaaaa agaactctta cagttctatg
     8641 ataatgaaat ctctagtgct tcacacattt ttcttacttc ttctcgatat ttaacaggta
     8701 cttggtcaat tgtttttaaa cctctctcta tcaatcctac ataaattgct gccatactac
     8761 ttcctccttc atttaatttt atatttttta atgacatagt agatgtctgt tgactactta
     8821 ataccatttc gcatatttca gcagtcgcta acattaaatc tgttatgtca ttatcttgcg
     8881 ttttttgact tgaatctaaa tcatctaatt cttttctttg tcttgacttc tcaatttttt
     8941 ctttatctat ttttatcatt taatcacctc ttaaagtgtt tcaacatcta aattgaataa
     9001 catagatggt ttttttatag ttgtattagt gtttccttct tctactatta ctttaggttg
     9061 tgcagattgt atattcatat cttttaactc atatatctcc ttatatccat tctcagtttt
     9121 tcttgtctct actttaacca tatctttaaa tgtatctgat tcatttatct ttaattgtac
     9181 ttttaattta tttactaaat gataaattgg attgttagaa tttaaatctt ctaacagacc
     9241 tttattgagt gtaagtacta catcttgtga attatcaact atactagata cattataagt
     9301 attcccatcg attaatacag ttgaatcttt tactatagtt gctcctgttt cttttttata
     9361 aatataaaca tttatatata aattatttcc aatgtcagaa tgtgctttaa atacaatctt
     9421 attttctcca agaactaaat tctctggatt aatctcaaac gtgactgttc ctgtagataa
     9481 gtcttctatt atacttgaac taatattatt tataaacatc tctatttttg tcaattcatt
     9541 atttggattt tgcaaatcta tttcaataga tgagtatgac ttattcttta tttccaaaca
     9601 agttttctct gaatctactt ttgagattat atataatgtt tcatctttta ttaaagcatc
     9661 cactatatca ttaacatctc ttggaatata tatttgtgag aaagtatata acatagaacc
     9721 aatctctaca tctttaattt tatatgtttc cttatttatc cctgtaacat acaaagtctt
     9781 atcatttttt ataacaaata tatgtttatc atcataagat tttatatatt ttacattttc
     9841 taatacttta gtaaataatt taataattgt attttttgta ttctcattca gtcctaaaat
     9901 cccatttata ttatatcctt gtacgtataa ttgaccttct gtagttatag ccaatatata
     9961 attactataa gcatatatct cccttatatt agaaatagtt aatacttctt gtaactcatt
    10021 aacctcatca ttatgcccta ttccaagttg atattctgag tttttaccaa tagaaaacaa
    10081 tgagccatca ttttttaaga agtaagtaaa attatcacca caagcaatat ctattatatt
    10141 tgttaagtta atttttataa attcagaagt tgttgttagc tcagatttat ttatagcaag
    10201 ttccccaaat tgatttgaac cacaaccata gacatcccca ttttctaata tcaaaattga
    10261 atgagttgat gataaagaca tcttttttat attatcaaaa ggcaatgaag ttgagtaatt
    10321 tccagcattt gtgccaaatg gtattctata attaatacta tcatcattat gtaaaaatat
    10381 cttcttattt actaatagtc cataattatt tcttataata gacatttcat tagaaaaact
    10441 taaatcacta ggattatcac cagaagtgtt tgcaatctga ttttcttcat gtccattaaa
    10501 aatacctact tgaccaacta cagcattacc ttttccttct aatttattat tagtatattg
    10561 aactataaga ccattatgct gtgtatatac ctgttttaca ttaaggcaat ttctaacagg
    10621 tgttttttgt acaacatcag aataattatt gatttgaccc aattgtctac taccaaacac
    10681 atataaggca ttatcattat ctatataata agcattttta ccatttgcat atatgtgctt
    10741 tatattactt gttaattttt ttaatactac agtaccagat tcattaaaac ctataccatc
    10801 atcagcattt cctccagaat ataaatctcc attttcaagt aaaacaaaaa tagtgtattc
    10861 tcctagagca acctctttta tattactatt aaacgctaaa tccttatatc cttcaatata
    10921 tcctgatgaa ttattatatg aagtaccaac acctaaacct cctaatgagt tatatcctgt
    10981 gacaaatatt ctatttttca ttgtaattat agttttgcct tcttccaaca ttaaatttga
    11041 tatagttgat gggtctgtta atccttcaat atttatttta gttaaactgt ttgaatttgt
    11101 atttgaactt atgtaatttc tatttccaca acccattaaa gaaattatat tatcattctc
    11161 caataatata ataaaaacat accttccaca aaatatatcc tttattgttc cagaaatatt
    11221 tactttagta aaagtatttc tattaatatt atcccctaat cctaattgac catagccatt
    11281 tgaaccagtt gaaaataatt cattgtcata ggttagaatg taagaagatt tgtaacatgt
    11341 tattactttt tttacattat caataggcac cttagtaaaa gtacccctat caatattatc
    11401 tcctaaacct aattgcccta agtcatttgt tccaattgaa tagacttgat tattatttgt
    11461 aagaataaat aaacaatcat tcgatggaat aaattctttt atattatcta taggaacttt
    11521 tataaattca gaccgagctt taattgtccc taacttgtta ccagcattat taccacagac
    11581 atatccttcg ctattattta ataatatata taaagcttgt ctatcacttt taatttcttt
    11641 tatatttgat atgatatatt ttttagaaaa ttgttttgat atatttgcaa aaccatcatg
    11701 tctaacatca taaggttttt ttatgccaaa acagccatga gaatttactc cagaacaata
    11761 tatgttccca aaataatctt taattactat ccttaatcta ctagcatata ttacttgtga
    11821 attatatgca aaaaaagcat ttgataatgt gtatttttga ctattagaat aataactact
    11881 gtctcctctt tgcccacctg catttctacc tcttatttca aatttagact ccatccttgt
    11941 tttacttgtt gtatataaaa gattgtctgt tccaagataa tgattataat aaggcataga
    12001 agacgatata agacttctta aacctaaatt cttgtcttgg gatgaagctc caaaagtcga
    12061 aggtcttcct gtgaatttta tttctttata tcctccatca ctagcatcta ataataaaca
    12121 taaattatag ttagaaacca gaacttttga taatttaggt tcatctatta aaactgcgac
    12181 ttcatttgca tttgagtttt tacttcctac aaactctcca aaccttgaat aataatcatc
    12241 agttacagaa tttttaatac ttgtgctata ataattatgt ctcccagaaa catagtagtt
    12301 attagacgta gttctaatta aacatccatt attaaaagga ataatttcag caatgttaga
    12361 tacatctgat actgttgtat ctgttttata tcttaaatat ttcatcttaa caaaaacgct
    12421 cctacttgag gtatcattaa gacttaattc gccaaaaaca ttatatcctg ctgcataaga
    12481 tattctgcta ttgtctatgc aatatgaaga atttgaatat gcaataattt catttacata
    12541 agttacagta cttactttag taaaaatatt tctattagta ttgtcattaa gtcctaactg
    12601 cccataattg tttaaaccag ttgaaaataa ttcattagta gttgttttta ataacgcatg
    12661 gttatctcct acagaaattt tgtcaacaga agaaatattt actttagtaa aagtatttct
    12721 attaatatta tcccctaatc ctaacactcc aaatacatta tatcctgttg aatataatgt
    12781 attatcactt agtaatataa acgtgaaata tgagcctata tttacatata tatcttttat
    12841 agtaactcct tcttctaatt ctaatggcac tctagtaaac tcataacaat gacgattatg
    12901 acctaaacct aaacctccat aagcgttgta tcctgctgcg tatagttcat taccattcac
    12961 aacaataaat gtagaggtgt catttgcttt cactaaagag atatccacaa aattattttc
    13021 aagccctata tctaaaactc tttcatcatt tttatttatt aataatctat tatcaataga
    13081 ctcaatatga ttacattctt caatatcaaa gtcctcaaat ttttcaaatc catatataaa
    13141 tttatcatat ctttttaaat aagaaaaact ataattgtta tttcctgtca tatacaattc
    13201 attgttgcta gttaatataa agcttatagc ataagcacat ttaattttac ttatattttt
    13261 aatactaata gaagattttc taggagtata tgttgatgta tcacaattta ttttatctcc
    13321 taaacgccca tctgggttgg ctccagttac atatatatca ttattattag ctatataaaa
    13381 tttatgatat ctaccacttt ctatattttt tacattagat atagatagtt ttgttaagac
    13441 tgttactgta gctcctccac caacaccaac ttgcccactt gaattatctc cacaaccata
    13501 gacatcccca ttttctaaaa gaaaatttac agcaccttga gcagtagaaa tctttattac
    13561 attatctaaa tttactttag taaaaatatt tctattagta gtgtcattaa gtcctaactg
    13621 cccataggaa tttgtacctg ttgaatagac ttcattattt aaagttaata tatatgaaca
    13681 actgactcct gcaaatattt ctttaacact caaattatca attttagtaa atgtatttac
    13741 agtagatgta tttcctaagc ctatccctcc aaatttatta ttaccacata cataaagctc
    13801 gttgtcattt gtaactaaca tagaatagcc attcccacaa gcaatttgtt ttacattact
    13861 tatatttatc ttagtaaaag tatttctatc aatattatct cccaaaccta actccccatc
    13921 tgtgtttaag ccagtagaat acacatctcc gttattttta agtaaaagag aatgagttcc
    13981 agaagatgaa acatacttaa cattgtcaat atctattttt gaaaaatatc ttataggtga
    14041 tactttattt ccaagtccta aattgccaaa aacattatat cctgctgcat ataactttaa
    14101 atcatcctta attataaaca cagaaccaga atccatatct gtaaatatat ctttaacatt
    14161 aaaagtatca aaatttttat taaactcttc tattttaaat tctttgtttc caaataaaga
    14221 acctctttgt aatagtttat tctttttcat ataattcccc ctatctcact tcgtaggaat
    14281 acttgtttat ttttatttca gtatcacctt tttgataaga ttcttctgtt ttagtttcac
    14341 tatagttgct tcctaaatca tgaatatatc ttattttgtt gcataaaaaa ggaactgtta
    14401 catttttaaa taatacagtt ccactatttg aaagtaactc taattgttgc ttaaatttat
    14461 taagctttaa accatctaaa ttacttatgt ttttagaatc atatagggta tcataccaat
    14521 acccagcttc atctgctgta gcttccttat ctaattctaa ttcaaataga attttaagta
    14581 tttccacaga cattttatta taattttctt ttaatatttt caattgctcg tttgtatttc
    14641 caccagattc aagaacttta acactagcat ctaattcatt taccgcatct gatatatttt
    14701 tagatactgt acttaattca ctcgaaccaa ctttactttg tattagattt attttctcta
    14761 agttagtagc tatattactt ttattttcat ttatcttagt tagactctca tcaaattttg
    14821 catcaatttt aatactagag taagtactat ctagtgttgt tatattatca tttattgtag
    14881 cttgtataac ttctttattt gtattaccat ttacaacagt tacttttaca tctatgttac
    14941 tttcattaaa tatttctatc gtattagaat ctactatagt ataagaatta gtcatacttt
    15001 tcttagttgc ttcatctaat attgctacaa atatattttc tgtaactaaa ttatgattaa
    15061 ctataagttt aaaatattct ccatcagcaa cccaatccga ttcaacataa gatttactaa
    15121 aagctacatt agttgcagca gaagtcattt ctgctatttt ctcgttaact ttctcaaagt
    15181 catatgttaa tctatctttt aaagtatcct ttacagttcc atcaacactt tctctagctt
    15241 gtattaattc gcctctagca tctaaactcg ctaaactatc aaacttatca tctaactcaa
    15301 ccattttatt tgctacagta gcttcaatat ctgtcttagc agtatttact tcaactattt
    15361 tgtcatctac ttcttttatc tttgtatcta attttgtagt actttcatca atacaatctt
    15421 ctacattaga tatagcttga tttacttcat caagtttgta atcaatttgc tcttggttat
    15481 ctgtaagttt atcttcaaat gtcttatcat acaaatcaaa agtattatta gctctagtgt
    15541 ctaagtcttg aaacttctta tccgtctgag cttggacatc aagcatttta tcatcgacta
    15601 tattgtctat ttcttcagac ttagcatcta tcatttcatc taattggagt tggcaatctg
    15661 taagtttatc atcaacttga atttgcatat tctgaatgga ttcttcacga ttagactctt
    15721 ggttttgacg tgttaactca ttaaactctc tttcctcttc ttgtatgttt cttatgtctt
    15781 cattataaat cctaatctgc tctgctttat ctctaacctc ttcatttcca agtatagtta
    15841 tttcaagttg ttcaactctt ttagttaact ctttattatt ttttataatc tcttctgctt
    15901 gagatattat taccaataat actccatatt catcagttga ggtgatatca tcatcattaa
    15961 tcataccctc ttcaactcta taataaagat ttgtagtatt aagtaaagtt ccatctttca
    16021 caagggatag ttgaaatgta tttataccaa cagaagccaa tgcttgtcgg ctaagttcta
    16081 tctctacaac accattttct gcatctacaa cttcacattc accattaact ttttgtccgt
    16141 ctgattttgt aatgctaact aagactctat catattcaga caaatctatt atttcatctt
    16201 tatttttcaa tattgcttca atataagctg ttttgacatc attttcatta taaataaaat
    16261 agtctagtaa tcttttattt attctataat tactaaaatc tacttctatg gtatgattct
    16321 ttatttcata atttttcaaa gtatcacatc ctattgttct ttattttgtt cttctagttc
    16381 ttttttcaag ttctcaattt cttcttgttg ttgcatgttg agtgctttat aaagtattag
    16441 ttcattatat aaacttttta tctcttcaaa tgctactgta tatgcttttt gtaaatttac
    16501 ttccatatta atatcctcct aaattattaa ttttctcgtt taattcatta attatcatct
    16561 ctaattcttc tcttttttgt atttcttctt gaagtgcagt tgccacaact gttgtataac
    16621 cagatgggct aaacattaat ccattttctt ctccatagtc atatagaaat gtttttccaa
    16681 cataactatc tttaaaatca ttagctataa accctatttg actatctgca attgtcctat
    16741 ctttttctgc tatataatca aatgttgctg gtttaaaatc atctcttata aaatctaaaa
    16801 atggtgttgg agtgatactt gaacgagttc tattcttaat atcttttatg tatactatat
    16861 tctctttaaa ctttttatca gattcactca gagtagaacg agcatataaa tatctccatt
    16921 tataacgctc acaacctaaa tcagttacag aacgctctgg ataaaatgaa tatcctacac
    16981 ctaaactatc tccatttgta aatgtaaaag tagctccacc acatcttaca aaagtatcat
    17041 ctctttcaac aataaagtgt tcttctccat caacataaat ctcagcagta tcatatccta
    17101 cccaaaaata attattgcta tcatatttta atcttattgc tgaggcttga ctagaaccat
    17161 ctgattgtct tgcatctatt gcaaatccag acctgctact tccaaataat ctgattattg
    17221 gttcatgtcc agatggagta gttaatatat cacatgctat agctccttga tttactgtta
    17281 gaccactcct gcttatctct acataatttg atatatcatt aaatccaaat tcaattttat
    17341 caggtttaat tcctacaatt gagccagagt tattccttgt aaccatcatg gttatatcat
    17401 ctttagttaa tttaatttca gcagcatttg ttttagttgc accatcaact tttgctactt
    17461 ctaattttat ttctttagct gtagcattta tctgtgaaaa ttcctctgat atacttccac
    17521 ccatacctgt tatataaatt ctatccactg tatttttaag ttcatttatc tttaagttat
    17581 attgagcatt tgcatttata gctaaaattc tgtctgaagg tgtaattata ctatctgaaa
    17641 taactgaatt aattatagag tttagattgg tacttgctgc atccaatgct gtttttgaag
    17701 tgtttattgc tactaagtct actccagtag ttctatcaat aagtttttgt agctctgtat
    17761 ataaacttgc tttgtctgta tctaataaat taagttcaat atcaatcttt tctttttctt
    17821 catctgtaac ttcattatca ttaaaataat tagatatatt agaactaaat gttcctaagt
    17881 ctgtatttaa cttaattata gctttattta tgttttctct taatgttata ttttcttgtg
    17941 aaccaccaaa atttttagtt acttctttta ctaatatatt tacattttca gttctctggt
    18001 ctataagaga catattttct tttatatgag atgcatcttc ttgtacctct aaaactgtct
    18061 gatgtatccc atctatgtct ttttcaataa ctacaaattt ttcattgtgt ttttcatctg
    18121 tttcatacaa tgcccagatt tgttcttcag tattttttct tgaattacca tcaaaagata
    18181 tattgaatat tgatgagttt gtaggaactt ctgtatgctt tccattatca tttacaacta
    18241 ctaaaaattg tgtatttcct tctggtaatc ttacattaga aacttttaat gtccctggat
    18301 tatcagcatc ccagtatata tattttttat ttgtttcttt atctactact tggtaaagtg
    18361 tgttattgta agtaacaatt actccagtaa ttctaatcca tgaatctgct ggtgaattat
    18421 ccaatatcaa tttacatcag ctccttttgt atatttttct taggaatata ttcatctggt
    18481 aggttaattc ttttcttctg ttgctgaata aataaatgtc ttttagagtt caatgttctt
    18541 gttgcatgtt cagctttagt caaataatca gctattgttc ttttgtcatc ttcttttatt
    18601 tttttattac ttaaagttaa ttctaaagta tccaattttc cattttttaa attttgagta
    18661 aaagatgtaa aatatactaa ttcttcttct ttagattctt tgctatataa cactataata
    18721 tctcctaaac tcaaatctcc cttccaatgt tgtctaaaat taatatctat aattctatct
    18781 aagaaattta taacatccag agtccattct cgtgttggaa tacatttcaa acttaattgc
    18841 cttttacctt ctgctattaa atcttcaact tttaaaaaag catcatttgt ataagtgtca
    18901 taatagagga actcatttaa ttcatctaat agaacctcat taaatattaa atatccatcc
    18961 tcatcagttg aagtctccct cttacaaaga atatttatat cttttatact ttcatttaac
    19021 ttagctattt cttcttctaa atgttttatt tgaacatcca atattacttt tttatcttct
    19081 agttcactta tctctactgc tattttagct gaatttactt catctttatg ttgtggatta
    19141 tcatatgtct tttttatatc ttttttctta cttatcattt ctattaccat ttgccattcg
    19201 ttgcttttac tatcacgttc tctttgtttc ttaagctttg tatctattaa ttctctccaa
    19261 attggctctc ttatttcatt catttcttga tatttcttta tagctttact aagttcttca
    19321 ctcatttctt tattgtctaa gaaataagaa taattctcaa tgtaatcata tcctgtaact
    19381 gtagctccta ttacattcat atcttcacta ccttcaagct tcagtctagt tactatatca
    19441 tcactatttg tagttctttc aagtgatttt atatagttat catgagataa atataactga
    19501 atattatctc caaaactatc tatatgatac aaattaacta aattattata agtatcaaaa
    19561 tcagctatac attcaaattg ttcttttagt tcattattaa aatagtctaa ccagttagaa
    19621 ttaatacttt cttgccatct taccttttct ctcttattac cttcactatc agtttcataa
    19681 gcaattgaat catctacatg gccaagtttc caacctgtct cttgttttaa ataatcatta
    19741 agagatataa tggatgcttc ttcatcttta gtaaacattt gtaatccata atcctcaata
    19801 tttacatcaa tctttcctag tttaacttct ttagatttag ctgttactac ttttaattta
    19861 tcgtctgtag tcacaacatt cttaactaca aaatattctt tattattaag acaaataaga
    19921 cgttcttctt ttacttcttc aaaaatggga tttattattt tattaaataa aaatctatct
    19981 gtaatatatt ttggaatggt cagttgtatt tcatctactc caccaagctc acgctttata
    20041 gaatttaaaa agttgattgg gatttgacct aaaaatgact tgttcatctt gtgaatggtt
    20101 aatgtgtaat cagattttat ctgtttaaga ttcacattaa aatgcatatt ttatcacctt
    20161 ctttgtatta gaaaagccca tcaattaaga tgagctaaaa tattttatta atctaaatat
    20221 tgcaacaagt ttaatagctt gttacatata tgataaagca aaaaacactc aaacgccaat
    20281 aagagtggtt tcaaaagata aattgatttt ttaattaact ttaaggaaac aatcactctt
    20341 tgtattaagt agattaaatt ccttactttt attatatcat aaatacgttt tttgtaaaac
    20401 agattcaata aataaaaaat cactattact gatatcaatt aatacatgtt ttaaataggt
    20461 atattataaa tcatttcatt attcaaaatt cacttcaata tcttcaaggt ctttttcatt
    20521 aatatttagg tctttcagaa tttcgttttg agatattagt tctttttcat ctataccttt
    20581 cattcttatt tctgtctcaa taagaagctt acaatcttct atattattta tcatttgtaa
    20641 cacctcctta aatagatacc agagtatgaa aaaaaccact tgtttaaagt ggcatagata
    20701 agtagtaatt cttgtgtaaa atttttaaat tttctgttac ctgtattata tctttattct
    20761 actcctgtcg tcaatataat attaaattta acattatgga aaaatatggt ataatatttt
    20821 tagagactac agtttagact attagggtga tggctcttta gttaaaatcc ccagtaagaa
    20881 agggggtgag actatggagt acttagtttt gtttttaata atgataatta tgattacaag
    20941 aataatcaat catttgacaa agctaataga tgcaatctta aacttgaaag catctattag
    21001 gaaactaaag aatcaccaga ctggcgggtc tagtgattct gaaagtaata cataaatcta
    21061 ttctttttat tgttaacttt aagaactaca ccctacagca aatagtttgt agtctctttt
    21121 ttatgtccct atgatataaa tttatatcat ttatattact attatactcc aatattatca
    21181 aaatagtcaa tgttgtcatt atcttcattg aaaaataaaa aagctatatt attaatagaa
    21241 taacttctta gaacacaata ttcttttaca attgtagaca atataatatt aaatttaaca
    21301 tattggtaat ttatggtata atatttttag agactacata aaaaaaacta ttagggtgaa
    21361 tcttcatata cataagcctt cccatgtcaa atcaagaggg gaggtgatat gatattatga
    21421 aatatctaat ttgtttgtta acgataataa catttcttac aactataatt attagtttga
    21481 ttaagttggt tcagaattta aatagactct ttaaagaaat agatgagttt aagaaaacgt
    21541 tgcaaaaaat taagaatcac ccgaacggca attcaggtga ttcttcctct aattaacact
    21601 tcgcttattt atgaagagac accctagcac aaatagttgt agtctctttt ttttatgtct
//
LOCUS       DAMJYR010000057        21464 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.057, whole genome shotgun sequence.
ACCESSION   DAMJYR010000057 DAMJYR010000000
VERSION     DAMJYR010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 21464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21464
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.057"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            300..1571
                     /gene="serS"
                     /locus_tag="PC471_002144"
     CDS             300..1571
                     /gene="serS"
                     /locus_tag="PC471_002144"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000884181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HDF2789272.1"
                     /translation="MLDIKRIRENLDCIKKAMERRGEKDFNLDEVVKLDDERRKILQE
                     VEVMKNELNTASKNIPNLIKEGKDVENEKIKLKELSDKIKVIDQNLKEVEDKMEYLLM
                     RIPNVPHPEVPQGETDEDNVEVRTWGKTTTFDFESKAHWEIGTELGILDFETASKITG
                     SRFTLYKGLGARLERALLNFYLDTNTKVNGYTEVIPPFMANRNSFLGTGQLPKFEEDM
                     FKIEGMDYFMIPTSEVPLTNIHANEILKFEQLPINYTAYTPCFRSEAGSAGRDTRGLV
                     RQHQFNKVEMVKIVAPEESYNELEKLTNNAETMLQLLNLPYRVVKICTGDLGFTASFK
                     YDVEVWMPSYNRYVEISSCSNCEDFQARRAGIRFKRDKDSKAEYVHTLNGSGLAIGRS
                     VAAILENYQQEDGSVVVPEVLRPYMGVSVIK"
     gene            1849..3354
                     /locus_tag="PC471_002145"
     CDS             1849..3354
                     /locus_tag="PC471_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HDF2789273.1"
                     /translation="MKIQNTEWGYIEWKHTYDENNPKQAMNIYIAVTMPGKKHFNHVH
                     YGQEQMIYILEGEGLYIINGVWKPFYQGMIFYIESGSTHETINTGDREIKELIVSNNV
                     DDVGESEVIDINPNNYLKKTLINYSESTLNLYAAVESIRGQFIDPFKIPLIIYDDSWN
                     IVLKNPYFPLFCFEKCNPMKFPQNCDCMNQKSSNQFVCEYGITIYNIPILYKSNSIGV
                     IRGGYVLLSDLNLDTEHNNLYDIPEGAARSIKRLLKQISKNIINFCSFNDIRKDLQEK
                     EKTIARTYHYGEQLEMNLKVAQDMVTNLRINHHFLFNTLNSMASIALDDGSYDLYSAI
                     IDLSRMFRYTMRSDLRFVELESEILYIKNYLNLQKLRYGDALKVKYLIPEKLYNLSVP
                     FNFIQPIVENAFTHGFRDIDTEKRIEIIARLDSQYAIIEIHNNGTILDGNNIDKIKAG
                     IRSNNGHGLSLIYTKFTSAYGNNFDMDIKSSDNEGTYIMIKIPIENYKEYV"
     gene            3468..4166
                     /locus_tag="PC471_002146"
     CDS             3468..4166
                     /locus_tag="PC471_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HDF2789274.1"
                     /translation="MRRALIIDDEEAVLKIITHFIEMKDMPIKIVGKATSGDEAVDKI
                     IGLEPDIVFIDIQMPIYNGLEVIEKTSHLSKKINFVVITAFNYFEYAQKALRLNVKDI
                     LLKPLDMKEFTKSVEKIIGYQYTNNDLLNEILEYINLNYSNDLKLNDCARLFLTNPSN
                     ISRIFKSNLNTTFISYLNHIRIEKSIELLENTTKSINEIAEIVGYNSLNNFYKNFKIE
                     KGMTPKVYKLNTKN"
     gene            4451..5539
                     /locus_tag="PC471_002147"
     CDS             4451..5539
                     /locus_tag="PC471_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2789275.1"
                     /translation="MSDKVNMKNIICIAGAYLATAIGSGFATGQEILQFFASQGYMGL
                     IGALISMVLFSFLGAEVITRGRELKLKEPTRIYTFYCGKILGKFYEWFSPIFLFGILI
                     VMISGAGSTLTEYYGINPYLGRVLMAVATLITVSMGLEKLVSILGRIGPVIAVFTIAV
                     GLISLFKHFDGLSMVGEAMKNIEVTKASESPIVSGVIYNTLNIIVMIVFLTGMGASIE
                     SKKDAFWGGLLGGIVFMTAGIVMYLAMISDIGNLYMKEIPSLYLADNISPIVGVCFSV
                     ALILGIYTTAVPLLWSVTNRIVEDEHPKFKLVTTVIAILACIGGFLPFGKLVNILYPY
                     TGYMGLLILVCMVYKAITCRKKSTQSQE"
     gene            5778..6968
                     /locus_tag="PC471_002148"
     CDS             5778..6968
                     /locus_tag="PC471_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HDF2789276.1"
                     /translation="MHELILISEKYKEDILDFFTDIHMHPELSFKEFRTTKAIKDLLV
                     SLDIEILDLGMETGVVGLLKGKYDGPTVALRGDIDALPIYEEVDIKYKSRYDGIMHAC
                     GHDIHTSCLVGCAYVLSHIRDSLHGNVKFIFQPAEEVNKGAKMLVERGVMENPKVDAI
                     FGLHNHPDIPCGKIGVKLGGLMAAVDTIKIEVNGFGGHGGIPNRTIDPIVASSAIIMG
                     IQTIVSRNISPLESAVISIGTINGGTANNVISEKVDMTGTCRSFSNEVRKKISENLEN
                     IVCEIARGYQATAKLDYLFDLPAVINSKEMYAIACKSVCDLYSEDAIVDPIPSTGGED
                     FSIFMEKAPGFFYWLGVGNKEQDCIYQWHNPKFKADKNSILVGTNVLCQSVINYMDKL
                     KNKI"
     gene            7059..8561
                     /locus_tag="PC471_002149"
     CDS             7059..8561
                     /locus_tag="PC471_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HDF2789277.1"
                     /translation="MRALNVVERAGNKLPDPVTIFLLLCIIVVILSAVISNLGVEEIH
                     PSTKEVVKVVNLLEKEQIQSYLGSIVTNFQSFAPLGLVLVTMLGAGVAEKSGFMEVLM
                     KKGISKVPQKLVTVAIVFAGMLSHTAADVGFIILPPLAALVFLGIGRHPLVGMFAAFA
                     GVAGGFAANVMLSTTDVLLAGFTIPAAQMMDPSYQGNPAMNLYFAFISAIVLTFAGAF
                     VTEKFIAPRFTKYNDSTNDKDIQELSELENKGIKYALLSLLVVVVVIVALCIGDNAFM
                     KDPETGSILSSNAPLMKGIVPIITIIFLTPGLVYGKVSKKIKSDKDLVSMMGSSMSDM
                     GGYIVLAFIASQFINLFNLSNLGTILSITGAKLLAESGIPSYGLIIGFILLSGFINLF
                     VGSASAKWAILAPIFVPMFMLLDFNPALTQIAYRIGDASTNPISPLFPYFPVILAFAR
                     RYDKDIGIGTVISNMIPYSVVFTLIEIIILLLFMGIGIPLGPGGGISYVL"
     gene            8633..9889
                     /locus_tag="PC471_002150"
     CDS             8633..9889
                     /locus_tag="PC471_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HDF2789278.1"
                     /translation="MLKTTKISDPELYKIVADELVRQEHNIEMIASESTAPTEVLELS
                     GCVFTNKTEEGYPGARFQAGSEEADKLETLAIKRAKEVFGAEHVNVQPYSGSTANYCV
                     YSSILKPNDTVLSMRLDQGGHLTHGSAVNFLHDIYKYEFYGVDPNTGRIDYDALEAKA
                     KECRPKLIIAGASSYPRLIDYERISKVAKEVGAYFMVDMAHVAGLVAAKVIPSPVPYA
                     DFVSSSTTKTFCGPRSGIVLCKAEHAKKLDKGVFPGTLGSIHLNTVAAKAFSLLYLST
                     DKFKKIMEQVVVNAQTLASELISHGFSIVSGGTDNHIVMVDLRSKNLTGKQFEKALEY
                     VGITVNKNVIPDDPQSPFVTSGVRIGLTSISQRGLKEKEVIQIAGIMNKVAENIDNKE
                     VLDECKAEAQELISKFPLYPEGYFED"
     gene            10061..12835
                     /locus_tag="PC471_002151"
     CDS             10061..12835
                     /locus_tag="PC471_002151"
                     /EC_number="7.2.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-translocating P-type ATPase, PMCA-type"
                     /protein_id="HDF2789279.1"
                     /translation="MRYYNKPTKEVLKYLKTNPEIGLDDNEVEERKLRYGLNEFTIKE
                     GRTFWDELGESLTEPMILILIGAAVISSFVGELHDALGILGAIFIGISIGIITEGKSK
                     KAAHALSKLTENIEVKVLRNGKIIKISKNDLVPGDIVYIETGDMIPADGRLIQSINLK
                     LREDMLTGESDDVAKNADAVLDMEVVYSKTEIIEQDAIPAKQVNMVFGGTLVAYGRGI
                     MVVTHTGDKTEMGKIAQNLSNEDQQTPLQIKLGKLGAKIAGISGIIATLLCMFMIIQM
                     QRKGMLILDTSSILSFLQSLEPAKNAYMVCIALIVATVPEGLPTMINITLAITMQKMA
                     KINALVTKKEACETIGSVSVICSDKTGTLTQNKMMVEVAYVDGKYISGGEYQSNSYFE
                     QNCIVNSTADIEKEDNSFKYIGSATECALLLYHNDKNYNEMRKQTYLISQIPFSSEEK
                     KMSTLIRQEDSDILLSKGAPEVLLKKCSYVQQGKNIVPITPKVEKSILDEIKKLQIKS
                     MRTLGFAYKKMSNSKTEVAMTSEGELNLIGNSRSYMKEDNLVFSGFVGIVDPLREGVK
                     DSIDKAFNAGVDVKMLTGDNINTATAIGNELGLLNDGKKAVEATYIDVLTDKELREEI
                     KGISIVARSKPDTKMRIVSALQKSGEVVAVTGDGINDAPALSQADVGIAMGISGTEVS
                     KNAADIILTDDSFSTIVEGIKWGRGIYENFQRFIQFQLTVNIVAFIIAIISQLTGKDM
                     PFTTIQLLWVNIIMDGPPALALGLEPVRDYVLKRKPINRHSGIIARSMFVNIIINAIL
                     IITIVFTQSAFNILGATSEEQGTVIFSLFAFSALFNALNCREFGLNSTIPNFFKNKLA
                     LQIIVVTGIIQIIFTQVFQSFFNSVSLDFDMWIKIILFASTILLSNEFVKLILRTMKN
                     SRTMNFNSKN"
     gene            complement(12900..13085)
                     /locus_tag="PC471_002152"
     CDS             complement(12900..13085)
                     /locus_tag="PC471_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HDF2789280.1"
                     /translation="MFFISDIYTKSPIKFDTPLQKEAYKILQKLDIDFECVDTDEAIT
                     MEDCVQINKKLNMKMVI"
     gene            13460..14638
                     /locus_tag="PC471_002153"
     CDS             13460..14638
                     /locus_tag="PC471_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HDF2789281.1"
                     /translation="MFSKILVKTFIRDSENVQSTDVRNKYGYVAGVVGILSNLLLFVI
                     KVFIGMLTSSIAIMADAFNNLSDMASSAITMIGFKLASKPADKEHPFGHGRIEYLSAL
                     IVAFMVMLVGLQFVKSSIERIVNPIPVKFEVIPLILLIASIMIKIWLSRFNKFMGNKI
                     DSSALKAVSLDALGDVFTSSCVVISFIVARFTNFPIDGYVGIVVSLVILYAGFSLVKD
                     TINPLLGEAPDEEMVNSIIELLLSYKYIIGTHDLIIHNYGVGRCIASIHAEIPSNIDI
                     MEIHEIIDTAEREISEKLDIYLVIHMDPICLEDKEVMSAKKELEEILKKNSLVKSMHD
                     FRIVGKGSKKNLIFDIVVNPSEFSKNMSEDDLKEDITKLVKEINPEYNCVIVVDKDFI
                     "
     regulatory      14765..14850
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01786"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01786"
     gene            14968..17886
                     /locus_tag="PC471_002154"
     CDS             14968..17886
                     /locus_tag="PC471_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021362860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SrtB-anchored collagen-binding adhesin"
                     /protein_id="HDF2789282.1"
                     /translation="MKKGNRKALLISLIMILSMVVSTIYPTVSYASELGENSQIQSGS
                     TNSSTGEEKESDNKKPEQTPEKDKATDNKKPEQTPEEEKPTDNKKPEQTPEEDKSTDN
                     KKPEQTPEGEKPIDNKKPEQIPEEDKSTDNKKSEQALEDEKPLDNKNTEKTPEEDNLL
                     ENENLLKVLEEELNEENEDYGFVVKINNNTIETESMKKISFNLTYTPTSKGIQAGDSI
                     TFKVPDVFNKVNLDYTSECFDKTESNGEYTLTFRELPNGQSVMQGKIGLEAYVKKVDE
                     DTNAKIHIETTGKIESGSGDIDVEIKPGDKTDVPDAKGTLKKLVEGKKSTTVFMPVKN
                     KDINYSIQVNEKQEELKDIILYDELPEGLTLINGSVSVVTSDGKEVSDFNIEQSKNSI
                     SVNLGNIDKSYTVKYKARISDKNAKHGNKYKNVARIESDGKKIQEDDATVSIFDRGDD
                     YLLTKGHSGATNITQVGQVINYQISINDDKSPISNVVITDNIPEGMRLTTSGEAGHDF
                     RVVEIPMNGSWTPWSKEKIANNISYKVEEKRNESGQVDKVITGFTINLSKEEVESKFF
                     IAYTLKVISIEDSYINRAVLDANNSEIDKNDEINFKKNSGLISAKKEVDKKVLNSSDN
                     QIVKYKINMSTYGVYDAGQVNLLDEVNSALEISNIKYSDNLELKKEAGDGKNTIRLVN
                     KYEFKQIKEGEPVQSWVTFDADFTNVKVGETIRNVAQINGSSPPGVETTKQGYAFEAK
                     KVDALDKNVLSGAKFNLEDAFGNIVVKDLVSDEDGIIQSSIKKPGTYYLVEIMAPSGY
                     EKLKDKIKVEIGNKDIGRIVDIGNIENIRQENPPVNPPAPPNTDEPIVNPPVPPSTDK
                     PRKPSSSSDTEDTIVINPPIPPSEDIINPPIPEVLNPPVPPSEEMIETPVKQIIPIPE
                     VVKPSVSEEKNNKVKDDTLVNPPVPPKTGDSTTIIGEILLVIGAIVGLIVLRRNKNTN
                     "
     regulatory      18072..18157
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            18257..18919
                     /gene="zmp1"
                     /locus_tag="PC471_002155"
     CDS             18257..18919
                     /gene="zmp1"
                     /locus_tag="PC471_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease Zmp1"
                     /protein_id="HDF2789283.1"
                     /translation="MRPSKKLLIAIISIFLISSVPVSAHADSTTIQQNKDTLSQIVVF
                     PTGNYDKNEANAMVNRLANIDGKYLNALKQNNLKIKLLSGKLTDEKEYAYLKGVVPKG
                     WEGTGKTWDDVPGLGGSTVALRIGFSNKGKGHDAINLELHETAHAIDHIVLNDISKSA
                     QFKQIFAKEGRSLGNVNYLGVYPEEFFAESFAYYYLNQDTNSKLKSACPQTYSFLQNL
                     AK"
     gene            complement(19041..20003)
                     /locus_tag="PC471_002156"
     CDS             complement(19041..20003)
                     /locus_tag="PC471_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HDF2789284.1"
                     /translation="MKKGYVYIILTTLFFSSMEISLKTVTNDFNPLQITLSRFFVGGL
                     VLLPFAVKRLRALSLSITKSDLKYFAFLGFMCVVVSMSLYQLAVLNTKASVVAVLFSC
                     NPVFVMLLAYLILKEKIYRHNVISLILEVLGIIVIINPLHTKLTLSGIFLTLSSAIIF
                     SMYTVFGKRKTLKFGGIVVTCFSFIFGSLEMLILVLLTKINFIADILNKNKLHVFANI
                     PIFSGYTLHNMPIMIYVFVFVTGVGYALYFMAMEATSTSLTSLVFFFKPVISPILALL
                     ILKEIIPINMVIGILLIVIGSIISIIPTIITQMHNKHVEALYKE"
     gene            complement(20173..21363)
                     /locus_tag="PC471_002157"
     CDS             complement(20173..21363)
                     /locus_tag="PC471_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HDF2789285.1"
                     /translation="MISNKMQTLVANSSVIRAMFEEGKKLSDIYGEENVFDFSIGNPS
                     VEPPETIKAVINDILNEESPNLVHGYMNNSGYEDVRDAIAEHINKKDGLNLTRENLIM
                     TCGAAGGLNIILKTLLNPGDEVIAFAPYFGEYKNYTENYDGKLIEVPTNIETFEPDLD
                     ALKNAITPKTRALIINTPNNPTGVIYSEELLKNLGELLDSKQKEFNTSIYLISDEPYR
                     EIIYDGAKVPCVLKYYRNSFIGYSYSKSLSLPGERIGYIVANGQMDDFDDVMSSLNVA
                     NRILGFVNAPSLFQRVIARSLDAEVDVNIYKKNFDLLYNSLIDMGYSCVKPNGTFYLF
                     PKAPIEDDKKFCNDAKQFNLLLVPGSSFGCPGHFRVSYCVSYDKVKSSLPAFEKLAKL
                     YNLK"
BASE COUNT         8476 a         2484 c         3678 g         6826 t
ORIGIN      
        1 tatataaaag ttaataacta tacaagattg ataaagaaaa agtcaaaata gataattgat
       61 tagcaaaata gatataatta taatgaagtt aaatctagca atattctcct aaaattaaat
      121 tacatttatt taattaaata taatttgaca atggacaaaa aagtgagtaa aatataaata
      181 aaataaaaat aacaggttcg gataaagaaa aaggtttctt aacctcaagc ataaagtata
      241 ttatctaagt tatttgagat taagagataa atccaatctt aggtactgag gaggaaaaaa
      301 tgcttgatat aaaaagaata agagaaaatt tagattgtat aaaaaaagca atggaaagaa
      361 ggggcgaaaa agactttaac cttgatgaag tagttaagtt agatgatgaa agaagaaaga
      421 ttcttcaaga agttgaagtt atgaaaaatg aattaaatac agcatctaaa aatataccaa
      481 atcttataaa agaaggtaaa gatgtagaaa atgaaaagat taaactaaaa gagttatctg
      541 ataaaattaa ggtaatagac caaaatctta aagaagtaga agataagatg gaatacctat
      601 taatgagaat accaaatgta ccacatccag aagtaccaca aggtgaaact gatgaagata
      661 atgttgaagt aagaacttgg ggaaagacta ctacctttga ttttgaatct aaggctcatt
      721 gggaaattgg aacagaatta ggtatacttg attttgaaac agctagtaaa ataactggtt
      781 caagatttac tctttacaaa ggattaggtg ctagattaga gagagcatta ttaaattttt
      841 acttagacac gaatacaaaa gtaaatggat atacagaagt tattccacca tttatggcaa
      901 atagaaatag ttttttaggt acaggacagt tacctaagtt tgaagaagat atgtttaaaa
      961 tagaaggtat ggactatttt atgattccaa catcagaagt tcctttaact aatatacatg
     1021 ctaatgaaat acttaaattt gaacaattac cgataaatta tacagcttat actccatgtt
     1081 ttaggtcaga agctggttct gctggaagag atacaagagg tttagtaaga caacatcagt
     1141 ttaacaaagt agaaatggtt aaaatagttg caccagaaga atcttacaat gagttagaaa
     1201 agcttactaa caatgcggaa actatgctac aattattgaa tctgccttat agggtagtaa
     1261 agatatgtac aggtgattta ggatttacgg cttcttttaa atatgatgta gaagtttgga
     1321 tgccaagtta caataggtat gttgagattt catcttgttc aaactgcgaa gatttccaag
     1381 ctagaagagc aggaataaga tttaaaagag ataaagattc taaagctgaa tatgtacaca
     1441 cattaaatgg ctctgggtta gctattggaa gaagtgttgc agctatatta gaaaattatc
     1501 aacaagaaga tggttctgtg gttgttcctg aagttttaag accatatatg ggagtttctg
     1561 ttattaagta agagttaaat tatgtatttt taagcaatac aataatatag aggtaataaa
     1621 aaataattaa tataaaagtg atacaataat atagaagtaa taaaaaaata gttaatataa
     1681 aagtgataca ataatataga agtaataaaa aaaaataatt aatataaaaa tgtgtacaaa
     1741 ctaatgaata ttgatatagt aagctatcat atgaaagagt atgtacacat tttttttact
     1801 aatttttcac aatatgaaca taaataattt acaggaaagg aatcttttat gaaaattcaa
     1861 aatactgagt ggggatatat tgaatggaaa catacttatg atgaaaataa tccaaaacaa
     1921 gctatgaata tatacattgc agtaactatg cctggtaaga aacattttaa tcatgttcat
     1981 tatggtcaag agcagatgat atatatttta gagggagagg gtttgtatat tataaatgga
     2041 gtttggaagc cattttatca aggtatgatt ttttatatag aatctggtag cacacatgaa
     2101 actataaata caggtgatag agaaataaaa gaacttattg tttcaaataa tgtagatgat
     2161 gtaggagaga gtgaagttat agatattaat cctaataatt atttaaagaa gaccttgata
     2221 aattatagtg aaagtacatt aaatttgtat gcagcagtag aatcaattcg tggtcaattt
     2281 atagacccgt ttaaaattcc attaattata tatgatgatt catggaatat agttttaaaa
     2341 aatccttatt ttccactttt ttgttttgaa aaatgtaatc ctatgaaatt tcctcaaaat
     2401 tgtgattgta tgaatcaaaa aagtagcaat caatttgtat gtgaatatgg gataactata
     2461 tataatatac caatactgta caaaagcaat agcataggtg ttataagagg agggtatgta
     2521 ctattatctg atttgaattt agatacagag cataataatt tatatgatat tccagaggga
     2581 gctgctagaa gtataaaacg cctcttgaaa caaatatcta aaaatataat aaatttttgc
     2641 tcttttaatg atataagaaa ggatttacaa gaaaaagaga agactatagc aagaacatat
     2701 cattatggag agcaacttga aatgaattta aaagtagctc aagatatggt tactaattta
     2761 agaataaatc atcacttttt atttaataca ttaaattcaa tggcaagcat agcactagat
     2821 gatggtagtt atgatttgta tagtgcaata atagatttat caagaatgtt tagatataca
     2881 atgaggtctg atttaagatt tgtagaatta gaatctgaaa tattatacat taaaaattat
     2941 ttgaatttac agaaattacg ttatggagat gcacttaaag taaaatatct tattccagag
     3001 aagttatata acctttctgt gccttttaac tttattcaac ctattgtaga gaatgcattt
     3061 acacatggat ttagagatat tgacacagaa aagcgaattg aaataattgc aagattagat
     3121 tctcagtatg ctattataga gattcataat aatggaacta tcttagatgg aaataatata
     3181 gacaaaataa aggcaggtat tcgtagtaat aatggtcatg gtttgtcttt aatatataca
     3241 aaattcacat ctgcttatgg aaataatttt gatatggata taaagtcgag tgataatgaa
     3301 ggaacttaca taatgattaa aattcctatt gaaaattata aggagtatgt atagaacttg
     3361 tgaaaaatta ataagtttta taaatgttaa gaaatatatt agatgttttt atgttgtttt
     3421 atatatctta cttttttata ttattaagct aaaagggagg tattctcatg agaagagcac
     3481 taataataga tgatgaagaa gctgttctaa aaataataac acattttata gaaatgaaag
     3541 atatgccaat aaaaattgtt ggtaaagcaa catctggtga tgaagcagta gataaaatca
     3601 ttggtttaga gccagacata gtttttatag atattcaaat gccaatatac aacgggttag
     3661 aagtaataga aaaaacatct catttatcta aaaaaataaa ctttgtagta ataactgcat
     3721 ttaactattt tgaatatgct caaaaagctt tgagattaaa tgtaaaagat atactcctta
     3781 aaccattgga tatgaaagaa tttactaagt ctgtagaaaa aattattgga tatcaatata
     3841 ccaataatga tttattaaat gagatactgg aatatataaa tctaaactat agcaatgact
     3901 taaagttaaa tgattgtgct agattatttt taaccaatcc atctaatata tctaggattt
     3961 ttaaaagtaa tttaaataca acttttatat catatctaaa tcatataaga atagaaaaat
     4021 ctattgagtt acttgaaaat actacaaaaa gtataaatga gattgcagaa atagttggat
     4081 ataatagcct aaataacttt tataaaaact ttaagataga aaaaggaatg actccaaaag
     4141 tttataaact gaacactaaa aattgaaatt gataaataaa aagtcaatat agataattgt
     4201 ttgagtgtgt ctgaataagt atactttaat tatgataaaa attaaagcgc tttaaacata
     4261 tttaataata ttaaacctta taaaagctgt acctatttat ataaaaaaat atatataaat
     4321 aaaaagaaaa agatatacaa aataaaaaaa gtgaattaaa tactgtttca aaaagacaga
     4381 attttcattt aatttactga gagtttatat ttcttaaagg aaggtaactt tataatggag
     4441 gtaatttaat atgtcagaca aagtgaatat gaaaaatatt atctgtattg caggcgctta
     4501 tctagcaaca gcaattggtt ctgggtttgc tactggtcaa gaaattctac aattttttgc
     4561 ctcacaaggg tatatgggtt taattggagc attaatatca atggtattgt tttcatttct
     4621 tggtgctgaa gttataacta gaggtagaga attaaaactt aaagaaccaa caagaattta
     4681 cactttttat tgtgggaaga ttttaggaaa attttacgaa tggtttagtc caatattttt
     4741 atttgggatt cttatagtaa tgatttcagg tgcaggctca actttaactg aatactatgg
     4801 tataaatcct tacttaggaa gagtattgat ggctgttgct actttaatta cagtgtctat
     4861 gggtttagaa aaattagtaa gcatacttgg acgtattgga ccagtgatag cagtatttac
     4921 aattgctgtt ggattaataa gcttgtttaa gcattttgat ggtttatcta tggttggaga
     4981 agctatgaaa aatattgaag ttacaaaagc atcagaaagt ccaatcgttt ctggtgtaat
     5041 atataataca ttaaatatta ttgtaatgat tgttttttta acaggaatgg gagcttctat
     5101 tgaaagtaaa aaggatgctt tttggggagg tttattaggt ggaatagtgt ttatgactgc
     5161 tggtatagtt atgtatttgg ctatgatttc agatatagga aatttgtata tgaaagaaat
     5221 accatcactt tatttagcag ataatatatc tcctattgta ggtgtatgtt tttcagttgc
     5281 attaatatta ggtatctaca caacagctgt tccactttta tggtctgtaa ccaatagaat
     5341 tgttgaagat gaacatccta aatttaaatt ggtaacaaca gtaatagcta ttttggcttg
     5401 tataggaggt tttttaccgt ttggtaaact agtaaacatt ttatatccat atacaggtta
     5461 tatggggctt ttaattttag tctgtatggt gtataaagca attacatgta gaaaaaaatc
     5521 aacacaaagt caggaataga gccacttatt aatagttttt atttaaagaa attttttata
     5581 taatccgaat ttaaaaagtc aaaatagtta aataaattgt aaaatatgat aatatattta
     5641 attaaaataa tatagtaaaa tataaatata aactaaaatt aaatttataa aaaatatctt
     5701 tataaagatt attaacttat ataatttgta gttaaattat aaaaaataaa aaaataaaat
     5761 tttggagagg tgttagtatg catgaactta ttttaatctc agaaaagtat aaagaagata
     5821 ttttagattt ttttacagat attcatatgc accccgaatt aagttttaaa gaatttagga
     5881 ctacaaaagc tataaaagat ttattagtga gtttagacat agaaatatta gatttaggaa
     5941 tggaaacagg agttgttgga ttattaaaag ggaagtatga tggtcccaca gtagcactaa
     6001 gaggagatat tgatgcattg ccaatatatg aagaggttga tattaagtat aaatccagat
     6061 atgatggtat aatgcatgca tgtgggcatg atatacacac gtcttgcctt gtaggatgtg
     6121 catatgtatt gtctcatatt agagatagtc ttcatggaaa tgtaaagttt atatttcaac
     6181 cagccgaaga agtcaataaa ggtgcgaaga tgctagtgga acgaggagta atggaaaatc
     6241 caaaagttga tgctatattt ggacttcata atcatccaga tataccttgt ggaaaaatag
     6301 gtgttaagtt aggtgggtta atggcagcag tagacactat taagatagaa gtaaatggat
     6361 ttggaggtca cggaggtata cccaacagaa caatagaccc tatagtagca tcaagtgcaa
     6421 ttataatggg tattcaaacc atagtaagca gaaacataag tccattagaa tcagctgtta
     6481 tatcaatagg tactataaat ggagggactg caaataatgt aatttcagaa aaggtagata
     6541 tgacaggcac atgtagaagc tttagtaatg aagtacgaaa gaaaatatca gaaaatttag
     6601 aaaatatagt ttgtgagata gctcgtggat atcaagcaac tgcaaagcta gattatttat
     6661 ttgatttacc agctgttata aatagtaaag agatgtacgc aatagcttgt aagtctgtgt
     6721 gtgatttgta ttcagaagat gctatagttg atccaatacc ttctacagga ggggaagatt
     6781 tttctatatt catggaaaaa gccccaggat ttttttactg gttaggggta ggcaacaaag
     6841 aacaagattg tatatatcag tggcataatc ctaaatttaa agctgataaa aattctatat
     6901 tggtaggtac aaatgtacta tgtcagtctg ttataaatta tatggataaa ttaaaaaata
     6961 aaatttaaga tagagcattc tggagataaa gtttatgagg gggtattagt ttgaggaaaa
     7021 ttaattcaca ggaaaaaaac aaagatagtg gattattgat gagggcacta aatgttgtag
     7081 agagagcagg aaataaactt ccagacccag taaccatatt tttattacta tgtataattg
     7141 ttgtaatatt gtcagcagtt atatctaatt taggagtaga agaaatacat ccttctacaa
     7201 aagaagttgt aaaggtagta aatctactag agaaagagca gattcaatca tatttaggta
     7261 gcatagttac aaactttcag tcatttgctc ctttaggatt agtactggtt acaatgcttg
     7321 gagcaggtgt tgctgagaaa tctggattta tggaagtgtt aatgaaaaag ggtataagta
     7381 aagttccaca aaaacttgta actgtagcaa tcgtatttgc aggtatgttg tcacatacag
     7441 cagcagatgt aggatttata atattaccac cacttgcagc acttgtgttt ttaggaattg
     7501 gaagacatcc tcttgtaggt atgtttgcag cttttgcagg tgttgcaggg ggatttgcag
     7561 caaatgttat gcttagtact acagatgtat tgttagctgg atttacaata ccagcagctc
     7621 aaatgatgga cccatcttac caaggcaatc cagcaatgaa tctttatttt gcttttatat
     7681 cagcgatagt tttaactttt gcaggagcat ttgtaactga aaaatttata gctcctcgtt
     7741 ttacaaaata taatgattca acaaatgata aggatataca ggaattgagt gaacttgaaa
     7801 ataagggtat aaaatacgct ttattatcat tgttagttgt agtagtagtg atagttgcct
     7861 tatgtatagg tgacaatgca tttatgaaag atccagagac tggttctata ctatctagta
     7921 atgcaccttt aatgaaaggt atagttccta taatcactat tatattttta actccaggat
     7981 tagtgtatgg aaaagtaagt aagaagataa aaagtgacaa agatttagta tctatgatgg
     8041 gtagtagtat gagtgatatg ggtggatata tagtcctagc atttatagca tcccagttta
     8101 ttaatttatt taatcttagt aacttaggaa caattttatc aataacagga gcaaaattac
     8161 tagcagagtc gggaatacct agctatggat tgataatagg atttatattg ctatctggat
     8221 ttatcaattt atttgtagga agtgcttctg caaaatgggc gattttagca cctatatttg
     8281 ttcccatgtt tatgctacta gactttaatc cagcattaac tcaaatagct tatcgtattg
     8341 gagatgcttc aacaaatcca ataagtccac tttttccgta ttttccagtt atattggcct
     8401 ttgccagaag atatgataaa gatataggta taggtactgt catatcgaat atgattccat
     8461 actcagtggt atttacatta atagaaataa taatattgtt actattcatg ggtattggca
     8521 taccattagg acctggtgga ggaatatctt acgttttata gaccgttata ataaatttat
     8581 caatgaagat taaactaata aaatatattc tttaattaag aggaggatta ttatgttaaa
     8641 aacaacaaaa atttcagacc cagagttata caagattgta gctgatgagt tggttagaca
     8701 agaacacaat attgagatga tagcttctga aagtacagca ccaacagaag tacttgaatt
     8761 aagtggatgt gtatttacaa ataaaacaga agaaggctat ccaggggctc gttttcaagc
     8821 aggttctgaa gaagcagata aacttgaaac cctagcaata aaacgtgcaa aagaagtatt
     8881 tggagcagaa catgtaaacg tacaaccgta ttcaggttct acagctaact attgtgtata
     8941 ttcatcaata ttaaaaccaa atgacactgt tttaagtatg cgtcttgacc aaggtggaca
     9001 cttgacacat ggtagcgcag ttaatttttt acatgacatt tataaatatg aattttatgg
     9061 ggtagaccca aatactggac gtatagatta tgatgctctt gaagcaaagg caaaagaatg
     9121 tagaccaaaa cttataattg caggggcaag ttcatatcca agattaatag attatgaaag
     9181 aatatcaaag gttgcaaaag aagtaggagc ttactttatg gtagatatgg cacatgttgc
     9241 aggattagtc gcagcgaaag taatacctag tcctgtacct tatgcagatt ttgtttcttc
     9301 atctacgaca aagactttct gtggaccaag aagtggtatt gtactttgta aagcagaaca
     9361 tgcaaagaaa ctcgataaag gtgtttttcc aggaacatta ggttctatac atcttaatac
     9421 agttgcagca aaagcctttt cactattata tttaagtaca gataaattta aaaagataat
     9481 ggagcaagtt gtagtaaatg ctcagactct agcttctgaa ctgatttcac atggttttag
     9541 tattgtaagt ggaggtactg ataatcatat agtaatggtt gacttaagaa gtaagaatct
     9601 tacaggaaaa caatttgaaa aagctcttga atatgtagga ataacagtta ataaaaatgt
     9661 tatacctgac gacccacaat caccatttgt aactagtggt gttcgtatag gtcttacatc
     9721 aatatctcaa agaggtctga aagaaaaaga agttatacag atagcaggga taatgaataa
     9781 ggtagcagaa aatattgata ataaagaagt attagatgaa tgtaaagcag aggcacaaga
     9841 acttatatct aagtttccat tatatccaga agggtatttt gaagattaaa tattttgatt
     9901 tgtatagctc acaatttata aagcctgtaa cataaaattt aataatatat aaccactata
     9961 aggtgttgat taatcaacac cttatttttt ttgaaaagta atatacaagc tatcataagc
    10021 atataattcc ttatagataa ttaacaaggg ggataaagtt atgaggtatt ataataaacc
    10081 taccaaagaa gtattaaaat acttaaaaac aaatccagaa attggacttg atgataatga
    10141 ggtagaagaa aggaagctta gatatggatt aaatgaattt actataaaag aaggtagaac
    10201 attttgggac gagttgggag aaagtttgac agaacctatg atattaattc tcataggagc
    10261 agcagtaata agttcttttg tgggagaatt acatgacgca ttaggtattt taggggctat
    10321 ttttattgga atttctatag gcataataac tgaaggaaaa tctaaaaaag cagctcatgc
    10381 cctgtcaaaa ttaacagaaa atatagaagt aaaggtgctt agaaatggga agataattaa
    10441 aatatcaaaa aatgatttag taccaggaga tattgtatat atagaaactg gggatatgat
    10501 accagcagat ggaagactta tacagtctat aaatttgaaa cttagagaag atatgctaac
    10561 aggagaatct gatgatgtag ctaagaatgc tgatgctgta ttagatatgg aagtagttta
    10621 ttctaaaaca gaaataatcg aacaggatgc aatacctgca aagcaagtca atatggtatt
    10681 tggagggact cttgtagcat atggtagggg cataatggtt gtcactcata ctggtgataa
    10741 gacagagatg ggtaagatag cacagaattt atcaaatgaa gaccagcaaa ctccacttca
    10801 aattaaatta gggaagttgg gtgctaaaat agcaggaata tcaggcataa tagctacttt
    10861 attgtgtatg tttatgatta tacagatgca gagaaaaggt atgttaattt tagatacaag
    10921 cagtatatta tctttcttac aatcattaga acctgctaaa aatgcatata tggtttgtat
    10981 tgcattaata gttgcaactg ttccagaagg tttgccaact atgataaata ttaccttagc
    11041 tataacaatg caaaagatgg caaagataaa tgcattagta acaaagaaag aagcctgtga
    11101 aactataggt tctgtttctg taatatgttc cgacaaaaca gggactttaa cacaaaacaa
    11161 aatgatggta gaggtagcat atgttgatgg taaatatata agtggaggag agtatcaaag
    11221 taatagctat tttgaacaaa actgtatagt aaattcaact gcagatatag aaaaagaaga
    11281 taattcattt aaatatatag gaagcgcaac agagtgtgca ttacttttat atcataatga
    11341 taagaactat aatgaaatga gaaaacaaac gtatttgata tcacaaatac catttagttc
    11401 agaagaaaag aagatgtcaa cattaattcg tcaagaggat tcagatatat tactttcaaa
    11461 aggtgctcca gaagtattat taaaaaaatg ttcttatgta caacaaggaa aaaatattgt
    11521 tccaataact ccaaaagtag aaaaaagtat tttagatgag attaagaagt tacaaattaa
    11581 atctatgaga acattaggat ttgcttataa aaaaatgagt aattctaaaa cagaagttgc
    11641 aatgacttca gaaggtgaat tgaatttaat aggaaattca agaagttata tgaaggaaga
    11701 taatttagtt tttagtggat ttgtaggaat agtggaccca ttgagagaag gggtcaaaga
    11761 ttccattgat aaagcattta atgcaggagt agatgtaaaa atgcttacag gagataatat
    11821 aaatacagca acagctattg gaaatgaatt aggattatta aatgatggga aaaaggcagt
    11881 tgaagcaaca tatatagatg tattgacaga taaagaattg agagaagaaa tcaaaggaat
    11941 atctattgtt gcaagaagta aaccagatac aaaaatgaga atagtttcag cacttcaaaa
    12001 aagtggcgaa gttgtagctg taacaggtga tggtataaat gatgccccag cacttagtca
    12061 agcagatgtt ggaattgcaa tgggtatttc aggaacagaa gtatctaaaa atgcagcaga
    12121 tataatattg actgatgata gctttagtac tatagtagag ggaataaaat ggggaagagg
    12181 tatatatgag aactttcaaa gatttataca gtttcagctt actgtaaata tagtagcatt
    12241 tataattgct ataatatctc aacttactgg taaggatatg ccatttacta caatacaatt
    12301 attatgggta aatataataa tggatggtcc tccagcccta gcattaggat tagaacctgt
    12361 tagagattat gtattgaaaa gaaaaccaat aaatagacat tctggaataa tagctaggtc
    12421 tatgtttgta aatattatta taaatgcaat tcttataatt acaattgtat ttactcaatc
    12481 agcttttaat atattgggag ctacatcaga agaacaaggt actgttatat tttctttatt
    12541 tgcattcagt gcccttttta atgcattaaa ttgtagagag tttggtttaa atagtacaat
    12601 accaaatttc tttaaaaata aactggcatt gcaaataata gtagttacag ggataattca
    12661 aataatattt acacaagtgt tccagagttt ctttaattct gtttctttgg actttgatat
    12721 gtggataaag ataatactat ttgcatccac tatcctattg tcaaatgaat ttgtaaagct
    12781 aattcttaga acaatgaaaa atagtagaac tatgaacttt aatagtaaaa attaattctt
    12841 atgaaaccgt aaagtactat atattgtaac tttgcggttt ttgtttgttt aatgttgttt
    12901 tatattacca ttttcatatt taatttttta ttaatctgaa cacaatcttc cattgtaatt
    12961 gcttcatctg tgtcaacaca ttcaaagtca atatctaatt tttgtaatat tttatatgcc
    13021 tctttttgca aaggagtatc aaatttaata ggtgattttg tgtaaatatc actgataaaa
    13081 aacatgattt atttcattcc tttccattta aaatcttata gttatagtgt atcataaggt
    13141 tataaaatat agataaatat agtataaatt taaaatatgt acagacaaaa caaaaaaatc
    13201 atattctaag taatacttgg gaaaaatgat attatcataa taaataatct ttgtaaaaac
    13261 ttatatagaa tctattaaac agacactaag ccataaaaac tttactaaat ataaaattcg
    13321 tatctaaata gatatataga ataatatttt actgaagtag agtgattttt aagaatttta
    13381 tatataaatt atgttatcat aaataaagat atagattata aaatttgtta ataaaataaa
    13441 gggcagggag gtaagtttca tgttttccaa aatcctagtc aaaacattta tcagagatag
    13501 cgaaaacgtt caaagtacag atgttagaaa taagtatggt tatgtagctg gtgtagtagg
    13561 tatattatcc aatttacttt tatttgtgat aaaggtattc ataggaatgc tcacatctag
    13621 tatagctata atggctgatg catttaataa tctttctgat atggcatctt cagcaataac
    13681 tatgataggt tttaaattag caagtaagcc agctgataaa gaacaccctt ttggtcatgg
    13741 aagaatagag tatttatcag cattaatagt agcatttatg gtgatgttgg ttggacttca
    13801 atttgtaaag tcatctatag aaaggatagt aaatccaatt cctgtaaaat ttgaagtaat
    13861 accacttata cttttaattg catctataat gattaaaatc tggttgagtc gttttaataa
    13921 atttatggga aataaaattg attcttcagc cttaaaagca gtttcacttg atgctttagg
    13981 agatgtattt acttcaagtt gtgtagttat atcatttatt gtagctagat ttactaattt
    14041 tcccatagat ggatatgttg gtatcgtagt atcattagtg atactttatg ctggtttctc
    14101 tcttgtaaaa gatactataa atcctctttt aggggaagca ccggatgaag aaatggtaaa
    14161 ttctattata gagctattgt tatcttataa atatataata ggtacacatg atttaattat
    14221 tcataactat ggagttggta gatgtattgc atctatacat gcagaaattc catccaacat
    14281 agatataatg gaaatacatg aaattataga tactgcagag agagaaatat ctgaaaaatt
    14341 agatatatat ctagtaattc acatggaccc aatatgtctt gaagataaag aagttatgtc
    14401 agccaaaaaa gaacttgaag aaatacttaa gaaaaatagt cttgtaaaat ctatgcatga
    14461 ttttagaata gttggaaaag gaagcaagaa gaatttaata tttgatatag tagttaatcc
    14521 ttccgaattt tccaaaaaca tgtcagaaga tgacttaaaa gaagatataa caaaattagt
    14581 taaggagata aatcctgaat ataattgtgt aattgttgta gataaggatt ttatttaatt
    14641 attaattaat ctcatcataa ggtaaaaaat gtaaaaagga tagacaatag tatagaggca
    14701 taatataata gaagtatata attgtatttt taatataaat aaagcaattt taattgtttt
    14761 tatgaaatat tatagagatg ttgaagtata ttctattatt gggcacctta tggatatact
    14821 gagtcagtgg tgcaaccggc tatgaatata aatttattta ttttcatagc tttttttgta
    14881 taatttttta gtaaaaatta ctttatatac gatattaatg gaagattaga ttataaatta
    14941 taggtaacct aaaggggtga gatttacatg aagaaaggaa atagaaaggc attattaatt
    15001 agtttaatta tgattttaag tatggtggtg tctacaatat atccaactgt atcttatgct
    15061 tcggaattag gagagaatag tcagattcaa agtggttcaa ctaattcatc aactggagag
    15121 gagaaggaaa gcgacaataa aaagccagaa cagactccag agaaagataa ggcaacagat
    15181 aataaaaaac cagaacagac cccagaggaa gaaaaaccaa cagataataa aaaaccagaa
    15241 cagaccccag aagaagataa gtcaacagat aataaaaagc cagagcaaac tccagaagga
    15301 gaaaagccaa tagataataa aaagccagag cagataccag aagaagataa gtcaacagat
    15361 aataaaaagt cagaacaagc tttagaagat gaaaaaccac tagacaataa aaatacagaa
    15421 aaaactccag aggaagataa tctattagaa aatgaaaatt tattgaaagt tttagaagaa
    15481 gaacttaatg aagaaaatga agactatgga tttgttgtaa aaattaataa caatacaata
    15541 gaaacggaat caatgaaaaa aatatcattt aatttaactt atactccaac atcaaaagga
    15601 atacaagcag gagattcaat aacttttaag gtgccagatg tattcaataa agttaattta
    15661 gattatacaa gtgaatgctt tgataaaaca gaaagtaatg gagagtatac acttacattt
    15721 agagaattac ctaatggcca aagtgtaatg caaggtaaaa taggtttgga agcatatgtt
    15781 aaaaaggttg atgaagatac taatgcaaaa atacatattg aaactactgg aaaaatagaa
    15841 agtggaagtg gagatataga tgtagaaata aaacctggag ataaaacgga tgttccagat
    15901 gctaaaggaa cacttaaaaa gcttgttgaa ggaaaaaaat caactactgt atttatgcca
    15961 gttaaaaata aagatataaa ttacagtata caagtaaatg aaaagcagga agagttaaaa
    16021 gatattattt tgtatgatga gcttcctgaa ggattgacat tgataaatgg ttctgtaagt
    16081 gtagtaacaa gtgatggaaa agaagttagt gattttaata ttgaacagag taaaaattct
    16141 atttcagtta atcttggaaa tatagataaa tcgtatactg ttaagtataa ggctagaatc
    16201 agtgacaaaa atgcaaaaca tggtaataaa tataagaatg tagctaggat tgaatctgat
    16261 ggtaagaaga tacaagaaga tgatgctact gtaagtatat ttgatagagg agatgattat
    16321 cttcttacta aaggacattc aggagcaaca aatattactc aagttggtca agtaataaac
    16381 tatcagattt caataaatga tgataaatca ccaataagta atgttgttat aacagataat
    16441 ataccagaag gcatgagatt aactacttca ggagaagctg ggcatgattt tagagttgta
    16501 gaaattccaa tgaatgggag ctggactcct tggagtaaag aaaaaattgc caataatatt
    16561 tcatataaag tagaagaaaa aagaaatgaa agtgggcaag tagataaagt tataactgga
    16621 tttacaatta atctcagtaa agaagaagtg gaatcaaagt tttttatagc ttatacatta
    16681 aaggtaatta gtattgaaga tagctatata aatagagctg ttcttgatgc aaataatagt
    16741 gaaattgata aaaatgatga gataaacttt aagaaaaatt ctggattaat aagcgcaaaa
    16801 aaagaagttg ataaaaaagt tttaaatagt agtgataacc agattgttaa atacaaaatc
    16861 aatatgtcaa cttatggggt ttatgatgca ggacaagtaa atcttttaga tgaagtaaac
    16921 tcagctctag aaatatctaa tataaaatat tctgataatc ttgaacttaa aaaagaagct
    16981 ggagatggaa aaaatacaat aagattagta aataaatacg aatttaaaca gataaaagaa
    17041 ggggaacctg tgcaaagttg ggttacattt gatgcagact tcacaaatgt aaaagttggg
    17101 gagactatac gaaatgtggc tcagattaat ggttcaagtc ctccaggagt agagactact
    17161 aagcaaggtt atgcttttga agctaaaaaa gtggatgctt tagataaaaa tgtactttca
    17221 ggtgctaagt tcaatttaga agatgcattt ggaaatatag tagttaagga tttagttagt
    17281 gatgaagatg gtataattca atctagtata aaaaaaccag gaacatatta tttagtggag
    17341 ataatggctc caagtggata tgaaaaactg aaggataaaa ttaaggttga aattggtaat
    17401 aaagatattg gtagaatagt ggatattgga aatatagaaa acataagaca agaaaatcca
    17461 ccagtaaatc caccagcacc accaaataca gatgaaccaa tagtaaatcc accagtacca
    17521 ccaagcacag ataaaccaag gaaaccatca agttcatctg atacagagga tactatagtt
    17581 ataaatccac caataccacc atcagaagat ataataaatc cgccaatacc agaggtattg
    17641 aatccgccag taccaccatc agaagagatg atagaaactc ctgttaaaca gattattcca
    17701 ataccagaag tagtaaagcc atcagtgtca gaagaaaaaa ataataaagt taaagatgat
    17761 actttggtaa atccaccagt accaccaaaa acaggggata gtactacaat tattggagaa
    17821 atacttttgg ttataggagc tattgtagga cttatcgtat taagaagaaa taaaaataca
    17881 aattagaccc taacattata aaaaatataa gttatattaa aaaatatctt ctaaaacaat
    17941 atttaataat gaaaatattg ttagaagata tttttttttg aagatttatg taaaaaaata
    18001 ataacttttt agaaaaaagt tccaaaggga tatagacaat taaaaagata taagctataa
    18061 tatagatata aaattaaatg aggcaaaact agggaaacct agtcacgcaa agctataggg
    18121 gctaaagttt agtaaaccat gccagccagt tgccgaaaag agtaaaaatt tctttttgta
    18181 tatacaaaga tactcttggg tatctttttt attttgaaat ttattactcc cggcctaact
    18241 caaaggaggg aataatatga gaccaagtaa aaaattatta atagctataa tatcaatatt
    18301 cttaataagt tcagttccag taagtgcaca tgcagatagt actactatac aacaaaataa
    18361 agacacactt agtcaaatag tagtttttcc aactggaaat tacgataaaa acgaagctaa
    18421 tgctatggta aatagattag ctaatataga tggaaagtat ttaaatgcat taaagcaaaa
    18481 taacttaaaa ataaaattat taagtggaaa gttaacagat gaaaaagaat atgcttattt
    18541 aaaaggtgta gttcctaagg gatgggaagg tactggaaaa acttgggatg atgttccagg
    18601 tttaggtgga agtacagtag ctcttagaat aggatttagt aacaaaggta aagggcatga
    18661 tgcaataaat ttagaattac atgaaacagc acatgcaata gaccatatag tattaaatga
    18721 tatatctaaa tcagcacaat tcaaacaaat atttgctaaa gaaggtcgtt ctttaggtaa
    18781 tgtaaactac ttaggtgtat atccagaaga attttttgca gaatcatttg catattacta
    18841 tttaaatcaa gatacaaata gtaagttaaa atcagcttgt ccacaaactt atagcttttt
    18901 gcaaaattta gctaaataga gttttaataa atttaaaata aatataaata aagagatgct
    18961 attataatag catctctttt atattgcgtt taaatctaat aaattttgtc ttatgtataa
    19021 ctatgataga ttatcaatta ctactcctta tacaaggctt caacgtgttt attatgcatt
    19081 tgagtaatta tcgtaggtat aatagaaatt attgaaccaa ttactatcaa taaaattcct
    19141 ataaccatgt ttatagggat aatttcttta agtattaata atgctagtat tggagatatt
    19201 actggtttaa agaagaatac taaagatgta agactagtag atgtagcttc cattgccata
    19261 aagtaaagcg cataaccaac acctgtaaca aatacgaata cataaatcat aatgggcata
    19321 ttgtgtaagg tatatccact aaaaataggg atatttgcaa atacatgaag cttattttta
    19381 tttaatatat ctgcgataaa attaatttta gtgagtaaaa ccaatatcaa catttccaaa
    19441 ctaccaaata taaagctaaa gcaggttaca actattccac caaattttaa ggtttttctt
    19501 ttaccaaata cagtatacat tgaaaaaata atagcagaag ataatgttaa gaatattcca
    19561 ctaagagtta gttttgtatg caatggattt attataacta taattccaag aacttctaat
    19621 ataagtgaaa ttacattgtg cctatatatt ttttccttca aaattaagta agctaaaagc
    19681 attacaaaga ctggattaca actgaacaaa acagcaacta cagatgcctt tgtgtttaga
    19741 acagcaagtt gatacaatga catacttact actacacaca taaaacctaa aaatgcaaag
    19801 tattttaagt ctgattttgt aatagaaaga ctaagagctc ttaatctttt tacagcaaaa
    19861 ggtagtaaga ctaaaccgcc aacaaaaaat cttgataaag ttatttgtaa gggattaaag
    19921 tcattagtga cagtttttag actaatttcc attgaactaa aaaataaggt agtcagtatt
    19981 atataaacat aacctttttt caagtcaaac acctccttgt aatttatatt agtactatag
    20041 ataagaatat tcaagcatat tggaataata tggtatgtat aaaatatagt acataaaatt
    20101 ataaactacc ttaatataga atgtaagtat tctatactaa ggtagttaat tatttgaatt
    20161 actttgattt agttatttta aattatataa tttggctaat ttttcaaatg ctggtaaaga
    20221 acttttaact ttatcataag atacacaata tgatactcta aaatgacctg gacaaccaaa
    20281 agaagaacct ggtactaata ataaattaaa ttgttttgca tcattgcaga attttttatc
    20341 atcttcaatt ggagcttttg ggaacaaata gaatgtacca ttaggtttaa cacaagaata
    20401 tcccatatcg attaaactat tatatagaag gtcaaaattc tttttataaa tatttacatc
    20461 aacttctgca tctaaacttc ttgcaataac cctttgaaaa agagaaggag catttacaaa
    20521 tccaagtatt ctatttgcaa catttaatga agacataaca tcatcaaaat catccatttg
    20581 cccattagca acaatatatc ctatacgttc accaggtaga gataatgatt tactataaga
    20641 gtatcctata aatgaatttc tatagtattt tagtacacaa ggaactttgg caccatcata
    20701 aattatttct ctatatggct catcagaaat taggtatatg cttgtattaa attctttttg
    20761 ttttgaatcc aataattctc caagattttt taataattct tcagaatata tgacaccagt
    20821 aggattattt ggagtgttta ttattaaagc tcttgttttt ggagtaatag cattttttag
    20881 agcatctaaa tctggttcaa aagtctctat atttgtagga acttctatta attttccatc
    20941 atagttttct gtataatttt tatactcacc aaaatacggt gcaaaagcaa taacttcatc
    21001 accaggattt aaaagagttt ttaaaataat atttagacca ccagcagcac cacaagtcat
    21061 aattaaattt tctcttgtaa gatttaatcc atctttttta tttatgtgtt cagcaatagc
    21121 atctcttaca tcctcatatc ctgagttgtt catatatcca tgaactaaat ttggagattc
    21181 ttcatttaaa atatcattta taactgcttt aatagtctct ggaggttcaa cacttggatt
    21241 tcctatacta aaatcaaaaa cattttcctc accataaata tcagaaagtt tctttccttc
    21301 ttcaaacata gctcttataa cagaactatt tgcaactaaa gtttgcattt tattagatat
    21361 catatgtaac actccttttt atagatttat attgcttatg tatatattac catattttta
    21421 ctgaatttag acaattttcg aaaaattact tgtttgaaaa tata
//
LOCUS       DAMJYR010000058        21289 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.058, whole genome shotgun sequence.
ACCESSION   DAMJYR010000058 DAMJYR010000000
VERSION     DAMJYR010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 21289)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21289)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21289
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.058"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            603..1634
                     /locus_tag="PC471_002158"
     CDS             603..1634
                     /locus_tag="PC471_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HDF2789286.1"
                     /translation="MENICDMNNKVKVAVLDTGIDKEHDYLKDNLVGGIAFECMHDYI
                     FISDKFDDEDGHGTACASIIKKEYEDVELFVIKILRKDGITNIKVLEEALRYLLDTNI
                     RLINLSLSVIGVESVKGLFEVCYELFRKGKIIVCSLANDFDLSYPAMFNNVIGVRGFT
                     LDGDDSFWYNKKYDVQCVMDSNSYISCDINNSYRLPPKCNSYLSAKLTGKIAKILSEK
                     PGITISDLNDKLESLATRNHWDSCDFDECSRIVNFKLDLYDKENALFMKVADVVRDCL
                     DIEENNEKLFKGSLFNKEIGLVYDNCFNLLKKLEHRFDIKFNYMDISKYDLVSIYTLT
                     ELVERYKNL"
     gene            1767..3065
                     /locus_tag="PC471_002159"
     CDS             1767..3065
                     /locus_tag="PC471_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HDF2789287.1"
                     /translation="MKYEEALEYISQTNKFGIRLGLENIGKLLELLGNPQETLNIIHV
                     AGTNGKGSVCSFVSNILRECGYKVGLYTSPYLETFTERIRVNGQNIPQDDVARIIELI
                     KEKIEIMVKEGYAYPTEFEVVTAMAFYYYSEQKVDFVALEVGLGGRYDATNIITKSLV
                     SVIVSISLDHTGILGDTIEKIAYEKAGIIKENGVVLVYDQTDEAKDVIKSVCKEKKAK
                     YIEVDFDDINIKKSDINSQIYDCTVMKETYRDLEIKLIGEHQINNSILAISVIKYLKD
                     INKLANINEESIRKGLINTKWPGRIEKIKENPIFIIDGAHNEDGAKSLAKALDKNFKG
                     RKLTLLIGMLEDKDIDSVLEILLSHFNKVITTTPNNPRAINSDILREKVLKYVDDVTS
                     KHEIEDAVNYTLETSSEDDIIISAGSLYMIGTVRTLVKKL"
     gene            complement(3179..3424)
                     /locus_tag="PC471_002160"
     CDS             complement(3179..3424)
                     /locus_tag="PC471_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03905 family TSCPD domain-containing
                     protein"
                     /protein_id="HDF2789288.1"
                     /translation="MKVTFNPSGVCCREMIFEVDENNVIVDAEFIGGCNGNLSGLRSL
                     IIGQNALEVADKLNGIDCGGKGTSCPDQLSKAIRQSI"
     gene            3572..4438
                     /locus_tag="PC471_002161"
     CDS             3572..4438
                     /locus_tag="PC471_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HDF2789289.1"
                     /translation="MKIYISNYLSKSISIVDYSTLELEKEIVLEDNIYPHHFCIEKEK
                     NLIYIPSSSNGILYVLDLSNDKIIDTVSIGGSLSQVVLSDNELFVANEDSNSIYVLDK
                     NTLNPIGIIGVDNMPHGFDFDRESKKLYVPCINSIVCIDTINKNIQKKINMDFRAWHI
                     ELDKQKKEMYISTLDGKLIIIDEVSMDIKKVLYEFLLPVEIRFNYSGKKVYVADLGYN
                     NVRILDYTTSRYIGNIEINGIPQGLEISKDEKLLFVSDTQENSVKVYETANNKLIKEI
                     KVGKEPTTIICL"
     gene            complement(4442..4978)
                     /locus_tag="PC471_002162"
     CDS             complement(4442..4978)
                     /locus_tag="PC471_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4364 family protein"
                     /protein_id="HDF2789290.1"
                     /translation="MFENSSEELAYHKLLILYILEKIKMDLTNSQITQVVLETEMMNY
                     FSLQQLLSQLMESKFLTTYKDSDREYYALTQRGVESLEYFFNRIPTSVTEKIDKYIDS
                     NKENLLADTQVKSSFVKQSDNEFIVNLRVIENQSNLIDLNLNVSSEKQAKLICNNWKN
                     NASYMYAEVIDLLIRDIH"
     gene            complement(5169..5372)
                     /locus_tag="PC471_002163"
     CDS             complement(5169..5372)
                     /locus_tag="PC471_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta-type small acid-soluble spore
                     protein"
                     /protein_id="HDF2789291.1"
                     /translation="MSNSNRTVVPEAKAALNQMKLEIANEIGLSNYENIDKGNLTARE
                     NGYVGGYMTKKLVEMAERQMAGK"
     gene            5663..6427
                     /locus_tag="PC471_002164"
     CDS             5663..6427
                     /locus_tag="PC471_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HDF2789292.1"
                     /translation="MKREVKRTKALIYSVLFFSLLLVLIFVFVYGIKYICSKSDNSKM
                     PIYKVDTNEKKIALSFDVAWGTDNMDDILKILDKHNVKATFFLVGSWVDDNEEIVKAI
                     DEKGHEIGNHSNTHANLKEISKEDITKEIETTSEKIYNITGKRTNLFRPPFGDVNNKA
                     MDICSDLGYKVIKWDVDSIDWKELGPNHVIEKVIKESQPGSIVLFHANINDVDNYLDT
                     IISRLKKDGYSLVKISDLLYKDNYVVDSNGVQKTKN"
     gene            6816..8129
                     /gene="prdC"
                     /locus_tag="PC471_002165"
     CDS             6816..8129
                     /gene="prdC"
                     /locus_tag="PC471_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline reductase-associated electron transfer
                     protein PrdC"
                     /protein_id="HDF2789293.1"
                     /translation="MNKLYSILLKQHVGGPDKPVVSVGDVVKKGTLIAEPTGLGANIY
                     ASVSGKISEINDQAIVIEADEVQEDTFEPLKGEGILDLIKEAGVVGMGGAGFPTHVKL
                     NIDLNGGTILANAAECEPLLAHNIKEIEERPEIVYQGIKYAMEVTNAGKGMLAIKSKH
                     PKAIEAFKKVIKPGDNIEVAELVDMYPMGEERAIVRDVLGKLLEPTQLPSEANAVVIN
                     VETLTRIVEAVEQKRPVISKNITVVGQLNSGKESIVFEDVPIGTTVGELIERAGGIKG
                     EYGEIILGGPFTGKATTLDAPITKTSGGIIVTMPFVNEKRKMGLLVCACGPNEERMRD
                     IATKMGVTDIVSVQKCKQAQEIKGALKCENPGHCPGQAQKCIEFKKAGAEVILIGNCT
                     DCSNTVMGSAPKLKLGVYHITDHVMRTVNHPLIRRIKGDVRLKIK"
     regulatory      8534..8619
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01786"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01786"
     gene            9201..11375
                     /locus_tag="PC471_002166"
     CDS             9201..11375
                     /locus_tag="PC471_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-Gln thioester bond-forming surface protein"
                     /protein_id="HDF2789294.1"
                     /translation="MKTKIKKSSIISLVIAFSMVFTAFTPIVSYADEVTSNDTILNGE
                     EQSNGQTPDVEKPSDGQVPDGEKPSDSQVPDGEKPSDGQVPDGEKPSDGQIPDGEKPS
                     DGQIPDGEKPSDGQIPDGEKPSDGQIPDGEKPSDGQMPDGEKTSDSQTPDVEKPSDGQ
                     MPDGEKPLDEQTPEEEKPLEEEIVIEEMSLKQDIDKILDMTLSQINKIVYNFWEDEED
                     VKADEQSEINQVFTSEDSFISLWYDKKAKVKNSCLLKEIDGSDRPYHDLRFDDITKTL
                     TFDYVIKGLVGASSKDDKYVIEGEDGEQTAFCYNNHLRPPSSNGKSPYLPAEDFNGQE
                     NQNEEAVKSILYAGSEFDGFGYKQQFNLGGEENELMTYSATQSAIWIILGQMDEQERL
                     KQYQGSINLCDKLIERAKTEEERAEYQKKKEVAINIKAYLEALLKAGREELKPNDTGK
                     PSLSNGLTKINFEKNEDGTYETEAVALVGYSGVVKLRLPDGVTAYDGDGNIIGTGEVE
                     ISTQQKFKLKSVVKPDAKANISAVSYDYIFPKAIQYYKAVLDLGQKDHSSSLPSSKQN
                     LLSYTIEKKNGQEVNFNIGLPTDDVVNPPVPPTDDTNGHKPKPSPPIDDTVINPPVPP
                     MDDTIINPPVPPTEDAVLNPPVPPTEDAVLNPPVPPTEDTVLNPPVPSVSDTVEKTPE
                     LSRDDTIIKSPKTGDETQLMSYVFISVIAICGLAYQCKIKRN"
     gene            11782..13542
                     /gene="prdR"
                     /locus_tag="PC471_002167"
     CDS             11782..13542
                     /gene="prdR"
                     /locus_tag="PC471_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator
                     PrdR"
                     /protein_id="HDF2789295.1"
                     /translation="MFSIPEVKKVEEVMDTKFTTIDEDTRIEDAIKEMIKSNTKTLMV
                     IDSSDQLKGIISMTDIHNLYEMHKKYEGQPVKLIMKKDVIYVNEGLTLDECRDIMILK
                     NIGILPVLRDNKIIGVLKQEHIRDYLYMHLEDYGLTLKYIIGQIKEGICAINNEGVVI
                     LWNKFMEERYDIKSEDIVGRPMNEFLENTISEKVLNSKVGMSDLYFTDKKENMYALVH
                     ANPIFYKEEFIGVVCTEVDVTEAKILALELEKVNDTLKYLKNEVKNLSKGSFDKILGK
                     SYKLEKSKAIAKQVARTNSSIFIWGESGTGKEVFARAIHDYSERKGQFIPVNCSAIPN
                     ELFESEFFGYESGAFTGASKKGRIGIFELAKDGTVFLDEIADLPLSMQAKLLRVLQEK
                     EIRRVGGDTTIKINPRIISATNKDLSKMVKAEKFREDLYYRLNVVEIKIPPLRERKED
                     IGLLVHSFLEEICKQNNKPVLTISKDVIDIFQNYRWKGNIRELKNTIENIVVLSQNSK
                     IDVDDVPSYMMDSTNNSTEEEEYPLDLTKATQKIEIKNITKALKMSNGNKAKAAKILN
                     IPRTTLYYKIDQYKIDVSKI"
     gene            13971..15851
                     /gene="prdA"
                     /locus_tag="PC471_002168"
     CDS             13971..15851
                     /gene="prdA"
                     /locus_tag="PC471_002168"
                     /EC_number="1.21.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-proline reductase (dithiol) proprotein PrdA"
                     /protein_id="HDF2789296.1"
                     /translation="MSITLETAQAHANDPAVCCCRFEAGTIIAPENLEDPAIFADLED
                     SGLLTIPENGLTIGQVLGAKLKETLDALSPMTTDNVEGYKAGEAKEEVVEETVEEAAP
                     VSEAAVVPVSTGVAGETVKIHIGEGKNISLEIPLSVAGQAGVAAPVANVAAPVASAAA
                     EVAPKVEEKKLLRSLTKKHFKIDKVEFADETKIEGTTLYIRNAEEICKEANETQELVV
                     DMKLEIITPDKYETYSEAVLDIQPIATKEEGELGSGITRVIDGAVMVLTGTDEDGVQI
                     GEFGSSEGELNTTIMWGRPGAADKGEIFIKGQVTIKAGTNMERPGPLAAHRAFDYVTQ
                     EIREALKKVDNSLVVDEEVIEQYRREGKKKVVVIKEIMGQGAMHDNLILPVEPVGTLG
                     AQPNVDLGNMPVVLSPLEVLDGGIHALTCIGPASKEMSRHYWREPLVIRAMEDEEIDL
                     VGVVFVGSPQVNAEKFYVSKRLGMLVEAMEVDGAVVTTEGFGNNHIDFASHIEQIGMR
                     GIPVVGVSFSAVQGALVVGNKYMTHMVDNNKSKQGIENEILSNNTLAPEDAVRIMAML
                     KNAIEGVEVKAPERKWNPNVKLNNIEAIEKVTGEKIVLEENEQSLPMSKKRREIYEKD
                     EN"
     gene            15932..16216
                     /locus_tag="PC471_002169"
     CDS             15932..16216
                     /locus_tag="PC471_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2789297.1"
                     /translation="MDRLKYISSETFYEGIIVDIKGGGVTIDLKGRLGQFKIPNRMLI
                     TDYELKIGQEVGFMLSYPEVLSPEPNQEYVENIRREKEKQAEMQSKQEEK"
     gene            16254..16979
                     /gene="prdB"
                     /locus_tag="PC471_002170"
     CDS             16254..16979
                     /gene="prdB"
                     /locus_tag="PC471_002170"
                     /EC_number="1.21.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011986824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:16704..16706,aa:Sec)
                     /transl_table=11
                     /product="D-proline reductase (dithiol) protein PrdB"
                     /protein_id="HDF2789298.1"
                     /translation="MSLTTVQGLQSEIFVPITPPPVWTPVTKELKDMTIALATAAGVH
                     LKSDKRFNLAGDTTFRAIPNTATVDEMMVSHGGYDNGDVNKDINCMFPIDRLHELAAE
                     GFIKGVAPMHYAFMGGGGNQHVFTEETGPAIAAKLKEEGVDGVVMTAGUGTCHRTAVI
                     VQRAIEEAGIPTIIIAALPPVVRQNGTPRAVAPLVPMGANAGEPHNIEMQTHILRDTL
                     EQLVAIPSAGKIVPLPYEYKAHV"
     gene            17059..17817
                     /gene="prdD"
                     /locus_tag="PC471_002171"
     CDS             17059..17817
                     /gene="prdD"
                     /locus_tag="PC471_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline reductase cluster protein PrdD"
                     /protein_id="HDF2789299.1"
                     /translation="MEEKIMRRLVIKPFHMNEVNFGSKTSIKKDVLTIDLSSIDEIKE
                     REELITDIKVDIIKPGDYDREINTIMDIIPISTKVLGRLGEGITHTLTGVYVMLTGAD
                     EDGNQMHEFGSSEGILKDQMVFGRYGTPSVEDYIIHVDVTLKGGLPFERTLPLAAFRA
                     CDDFIQEIRASLKMEDGRNATQVREYFDKIRPNAKKVVIVKQIAGQGAMYDNQLFSKE
                     PSGFEGGTSIIDMGNVPMIISPNEYRDGALRAMT"
     gene            17832..18299
                     /locus_tag="PC471_002172"
     CDS             17832..18299
                     /locus_tag="PC471_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline reductase"
                     /protein_id="HDF2789300.1"
                     /translation="MGIGPSTKETSLHHFRDPLLDIVESDKDVDLLGVIVVGTPDGNE
                     NKTFVGQRTAAWLEAMRVDGAIVSSDGWGNSHVDYANTFEEIGKRDIPVVGVTFNGTQ
                     AKFVVSNQYMDTIVDMNKSKEGIETEVVGENNTNEIDAKKALAFLKLKMRKHG"
     gene            18317..18799
                     /locus_tag="PC471_002173"
     CDS             18317..18799
                     /locus_tag="PC471_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline reductase"
                     /protein_id="HDF2789301.1"
                     /translation="MSFVGISTSMKNTSLHIFRDPLLDLIDNDSDIDLLGVVVVGTSE
                     HNEWKTFLATRLGRWIESLRPDGVIITLDMAGNQHIDFTNAIAEFVKSDIPTVGLTIM
                     GADGLVITNPYLDKATIIDYKKTTGYIETEVVGDNHMDEVDVKKALAFLKLKMRKDAK
                     "
     gene            18819..19826
                     /locus_tag="PC471_002174"
     CDS             18819..19826
                     /locus_tag="PC471_002174"
                     /EC_number="5.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline racemase"
                     /protein_id="HDF2789302.1"
                     /translation="MKFSRSIQAIDSHTAGEATRIVVGGIPNIKGNSMPEKKEYLEEN
                     LDYLRTAIMLEPRGHNDMFGSVMTQPCCPDADFGIIFMDGGGYLNMCGHGTIGAMTAA
                     IETGVVPAVEPVTHVVMEAPAGIIRGDVTVVDGKAKEVSFLNVPAFLYKEGVEVDLPG
                     VGTVKFDISFGGSFFAIIHASQLGLKIEPQNAGKLTELAMKLRDIINEKIEIQHPTLA
                     HIKTVDLVEIYDEPTHPEATYKNVVIFGQGQVDRSPCGTGTSAKLATLHAKGELKVGE
                     KFVYESILGTLFKGEIVEETKVADFNAVVPKITGSAYITGFNHFVIDEEDPLKHGFIL
                     K"
     gene            19946..20827
                     /locus_tag="PC471_002175"
     CDS             19946..20827
                     /locus_tag="PC471_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HDF2789303.1"
                     /translation="MVAILKALYVVFAAIFAVVFGKDVAKANKEGKLEEQSTPKVAVT
                     GFITDFFDTLGIGSFAPTIAMSKALKLNIPDKKMPGTLNVAHTIPVVTEAFIFTSIIP
                     VDGVTLVSMVVAAAVGSYIGAGIIAKMDERKIQLVMGVTLALTAILMLLGHPWINVLP
                     GGGNELGLTGAKLVIGVIGNFILGALMTAGIGLYAPGMAMVYFLGMSAKVAFPIVMGS
                     CALLMPVASMKFIKEDAYTKKASVIIAICGLIGVFVAAYIVKTLPMDILKALVIVVIA
                     YTSATMIMAANKNKKTA"
BASE COUNT         8498 a         2451 c         3891 g         6449 t
ORIGIN      
        1 tccttttatg caatttattt attgaaatat gatataatat ataaaggtgt aagtattagt
       61 tgttaaaata atatttatat tagtttttat acatacttgg tataagtttt tcatatattg
      121 attaaaattg atgttgattt attattttta gtattttttc accttttgat tcatactttg
      181 tagaatggtg tattttctaa aactgattat aagacaagtt cattcaaatt agcattttta
      241 tatcatataa ataattgttc cccctaaata agatttagat gtatagtatt aaaatcaaaa
      301 agtataaaat aaaattgatg cttaatcata gataaatatt aataaaatat acaaggggat
      361 tgtctaataa aaagataatc tcctttttta caaataattc taaattgtga tataaatcta
      421 aaaattttat cttaacattt aaccatttaa tgataataat tgatataata aatataaatt
      481 aaatatatgg tgtacttatt ttatgttttt agcaagaaat atagcattta aaagataaat
      541 tgtgcaactt aaatcaaagt ttaagatgaa ctattaacat agatatatta aaaacttttg
      601 taatggagaa tatatgtgat atgaataata aagttaaggt tgcagtatta gatacaggaa
      661 tagacaaaga acatgattac ttaaaagata atttagttgg aggaattgct tttgaatgta
      721 tgcatgatta tatctttata tcagataaat ttgatgatga agatggtcat ggaactgctt
      781 gtgcatcaat aataaagaaa gagtatgaag atgtagagtt atttgtaata aaaattttga
      841 gaaaagatgg tattacaaat ataaaagttc ttgaagaagc attaagatac cttttagaca
      901 ctaacataag attaattaat ttaagtttat cagtcatagg agtagaaagt gttaagggct
      961 tatttgaagt ttgctatgaa ttgtttagaa aaggaaaaat aatagtatgt tcactcgcta
     1021 atgattttga cttaagttat ccagctatgt ttaacaatgt tataggtgta agaggattta
     1081 ctttagatgg agatgattca ttttggtaca acaaaaaata tgatgttcaa tgtgttatgg
     1141 atagcaattc atatataagt tgtgatataa ataattccta tagattacca ccaaaatgta
     1201 atagttattt atcagcaaag cttactggta aaatagctaa gatattatca gaaaaaccag
     1261 gcataacaat atctgattta aatgataagc ttgaatcatt agcaactaga aatcattggg
     1321 atagttgtga ttttgatgag tgtagtagaa ttgtaaactt taaattggat ttgtatgaca
     1381 aagaaaatgc tctttttatg aaagttgctg acgttgttag ggattgctta gatattgaag
     1441 agaataatga aaaactcttt aagggtagcc tttttaataa ggagataggc ttggtttatg
     1501 ataattgttt taatctgttg aaaaagctag agcatagatt tgacataaag tttaattata
     1561 tggacatctc aaaatatgac ttagtttcaa tatatacact gactgaatta gtggaaagat
     1621 ataaaaactt ataaataaag atttgtagct aaatttaatg tagaaggtat taaattatat
     1681 gtattctttg tcggtagatt aaaagtaaaa aatatactat aattagatag cataagagaa
     1741 aatgccaata cagggggagt tagataatga aatatgaaga agcattagag tacatatcac
     1801 aaactaataa attcggtata agattaggat tagaaaatat cggaaagtta ttagaattat
     1861 taggaaatcc acaagaaact ttgaatataa tacatgtagc tggaactaat ggaaaaggtt
     1921 ctgtgtgttc ttttgtttct aatatactaa gagagtgtgg atataaagtt ggtttatata
     1981 catctccata tttagaaact tttactgagc gtataagagt aaatggacaa aacataccac
     2041 aggatgatgt tgctagaata attgagttaa taaaagaaaa aatagagatt atggtaaaag
     2101 aaggctatgc atatccgaca gaatttgaag tggttactgc aatggcattt tattactatt
     2161 cagaacaaaa ggtagacttt gtggcattag aagttggttt aggtggcaga tatgatgcta
     2221 caaatataat aacaaaatca cttgtaagtg tgatagtatc tataagtttg gaccatacag
     2281 gtatacttgg agatacaata gaaaaaatag cgtacgaaaa ggctggaata ataaaggaaa
     2341 atggagtagt actagtatat gaccagactg atgaagcaaa ggatgttata aagtcagttt
     2401 gtaaagagaa aaaagctaaa tatatagaag ttgattttga tgatataaac ataaaaaaat
     2461 cagatataaa ttctcaaata tacgattgta ctgtaatgaa agaaacttat agagaccttg
     2521 aaataaaatt gataggcgaa catcaaataa acaattctat cttggcaatt agtgtaataa
     2581 aatatctaaa agacataaat aaattagcaa atataaatga agaatctata agaaaaggac
     2641 ttataaatac taaatggcca ggaagaatag aaaagataaa ggaaaatcca atttttataa
     2701 tagatggtgc acataatgaa gatggagcaa aatctttggc aaaagcactt gataagaatt
     2761 ttaaaggtag aaaattaacc ttattaatag gtatgttaga agataaagat attgatagtg
     2821 ttttagagat attgttgtca cattttaata aggtaataac aacaacacca aataacccaa
     2881 gggccataaa ttcagatata ttaagagaaa aagtattaaa atatgtagat gatgtaacat
     2941 caaagcatga gatagaggat gcagtaaatt atacattaga aacatcaagt gaggatgata
     3001 ttataataag tgcaggttca ttatatatga ttggtacagt gagaacttta gtaaagaaat
     3061 tataatttta taaaataagt atacaaataa aaatataaaa aagagttgtc taaaaaatgg
     3121 acaactcttt ttatataaaa taagttttta ataattttac tgatgtttat ataagtaatt
     3181 atatagattg acgtatagct ttagataatt ggtctgggca agaagtacct tttccaccac
     3241 agtcaatacc atttaattta tcagcaactt caagagcatt ttggccaatt attaagcttc
     3301 taagtcctga taaattacca ttacatcctc ctataaactc agcatcaaca atcacattat
     3361 tttcatcaac ttcaaaaatc atttctctac aacatacacc acttggatta aatgttactt
     3421 tcattaaaaa gacctccatt aaatattatt tcttttttta acattaataa taataccaaa
     3481 tatatgcgta ataatgcaac tataaagcat aatacaagct aatgaaataa tatattattg
     3541 tagtatatgt attgtagaga ggaggggtta tatgaaaatc tatatttcta actacttatc
     3601 aaagagtatt agcatagttg attattctac acttgaacta gaaaaagaaa tagtattaga
     3661 agataatata tatccacacc atttttgcat agaaaaagaa aaaaatttaa tatacatacc
     3721 tagttcaagt aatgggatac tctatgtctt agatttaagt aatgataaaa taattgatac
     3781 tgtctctata ggaggaagtc ttagtcaagt tgtacttagc gataatgaat tgtttgtggc
     3841 aaatgaagat tctaatagta tatatgtact agataaaaac acattaaatc caataggaat
     3901 tattggagtt gataatatgc cacatggatt tgactttgat agagaaagca aaaagttata
     3961 tgtaccgtgt ataaactcga tagtatgtat agatacaatt aataaaaata tacaaaagaa
     4021 aataaatatg gactttagag cttggcatat agaacttgat aaacaaaaga aagaaatgta
     4081 tatatcaaca cttgatggaa agcttataat aatagatgaa gtaagcatgg atataaaaaa
     4141 ggtattatat gaatttttat taccagtaga aataagattt aattatagtg gcaaaaaagt
     4201 ttatgttgct gatttgggtt ataataatgt aagaatttta gattatacaa cgagtagata
     4261 cattggaaat attgaaataa atgggattcc ccaaggatta gaaatatcta aagatgaaaa
     4321 gttactattt gtatcagata cacaagaaaa ttcagtaaaa gtttatgaaa ctgcaaataa
     4381 caaattaata aaagaaatca aggtaggaaa agagcctaca actataattt gtttgtaggc
     4441 tttaatgtat gtctcttata agtaaatcga taacttcggc atacatataa gatgcattat
     4501 ttttccagtt attgcaaata agttttgctt gtttctctga tgaaacattt agatttaaat
     4561 ctattaagtt agactgattt tctatgactc taaggttgac aataaattca ttatcacttt
     4621 gttttacaaa actagacttt acttgagtat ctgctagtag attttctttg ttagaatcta
     4681 tatacttgtc tattttttca gtaacactag taggtattcg attaaagaag tattctagac
     4741 tttctacgcc tctttgagta agagcataat attctctgtc agagtcttta tatgtagtca
     4801 aaaacttaga ttccataagc tgagataaaa gctgttgtaa tgaaaaataa ttcatcatct
     4861 cagtctctaa tactacttga gttatttgag aattagttaa atccatttta attttttcta
     4921 atatatataa aattaaaagt ttgtgatagg ctaattcttc agacgagttt tcaaacataa
     4981 atatcatcct ccgtcaaact atttctatat ataatatatt aaactaattt gtacaaaatt
     5041 aaaagaacaa tttgtattgt attattaatc tttaataatt catataaaaa caattttata
     5101 aataaatata gaaaaacagg gctgctaatt agcagccctg ttgataccca ggtgtcaaaa
     5161 tttactattt attttccagc catttgtctt tcagccattt caactaattt cttagtcata
     5221 tatccaccaa catatccatt ttctctagct gttaagttac ctttgtctat gttttcatag
     5281 ttagataaac ctatttcgtt agctatttct aatttcattt ggtttaaagc agcttttgct
     5341 tctggaacta ctgttctgtt tgaatttgac atagtaaatt tcctcctcaa ataattagtt
     5401 tatgatgtct aacaacactt ttatttatgt gatgttaata ctaatttctc caaaataaaa
     5461 ataatatata cagaaaaata aaggtaaaaa tgtaaaaaat ataaaaaaat taaataaatg
     5521 agtattttag agagtttaat taggtcaata taggttttat ttttttagtt attgtcttat
     5581 aaattaaata ttacagtact tttttgagct gaaaaaaaat aaattttaaa tatagaagaa
     5641 attatttcaa agggggaata gtatgaaaag agaagttaaa aggactaaag ctttgatata
     5701 tagtgtttta tttttttcat tattattagt gttaatattt gtttttgttt atggaataaa
     5761 atacatatgt tctaaatctg acaattcaaa aatgcctata tataaagttg atacaaatga
     5821 gaaaaaaata gctttgagtt ttgatgtagc atggggaact gataatatgg atgatatttt
     5881 aaaaatttta gataaacata atgtaaaagc taccttcttt ttagttggta gttgggtgga
     5941 tgataatgaa gaaattgtaa aagctataga tgaaaaagga catgaaattg gaaatcattc
     6001 aaatactcat gccaatttga aggaaatatc aaaagaagac ataacaaaag agatagaaac
     6061 tacatcagaa aaaatatata atataacagg aaaaaggact aatttattta gacctccttt
     6121 tggggacgta aacaataaag ctatggacat ttgtagtgat ttaggttata aagtaattaa
     6181 gtgggatgta gattctatag actggaagga gcttggacca aatcatgtaa ttgaaaaagt
     6241 aataaaagaa tctcaacctg gttcaatagt tttattccat gctaatataa atgatgtaga
     6301 taactatttg gatactataa taagtcgact aaaaaaagat ggatacagtc tagtcaaaat
     6361 atctgatttg ttgtataaag ataattatgt agttgattcc aatggagtgc aaaaaactaa
     6421 gaattaataa gtaataaatg tatattttaa gtaaagttgt gataaattat atagtatatg
     6481 ttagaaaagt gtcgataaaa agacggtgtc tattttttga cactttttat acatgtatgc
     6541 aatatcaata attatagatt aaattgatta aaaaagtgtc taataattga cttttttttg
     6601 atgggtttaa aaatttagaa gaattaatga atgctgtaag acagtatttc caaagggtat
     6661 aagtttgttt aaaagttggc atagaaattg ctttgttaat aagcgatatg ttctatagaa
     6721 ataaacaata tgttaagacc ttataataga aaaatagcat taacattatt tgaacagctt
     6781 ataaaaaaat taatatactt aagggggcgt actagttgaa caaactatat tctattttat
     6841 taaaacaaca cgttggtggt ccagataagc cagtagtaag tgttggagat gtagtaaaaa
     6901 aaggaactct aatcgcagaa ccaactggat taggggcaaa tatttatgcc agtgttagtg
     6961 gaaaaataag tgaaataaat gaccaagcga tagtaattga agctgatgaa gtacaagaag
     7021 atacttttga accattaaaa ggagaaggta tacttgactt aattaaagaa gctggagtag
     7081 taggaatggg tggagcagga ttccctactc atgtaaaatt aaacatagat ttaaatggtg
     7141 gaacaatatt agcaaatgca gctgaatgtg aacctttatt agctcataat ataaaagaaa
     7201 tagaagaaag accagaaata gtttatcaag gtataaaata tgctatggaa gttactaatg
     7261 ctggaaaagg tatgctagca ataaaaagta agcatccaaa agcaatagaa gctttcaaaa
     7321 aagtaataaa gccaggagac aacatagaag ttgcagaact agttgatatg tacccaatgg
     7381 gagaagagag agcaatagta agagacgttc taggaaaatt acttgagcca actcaattac
     7441 cttcagaggc taatgcagta gtaataaacg tagaaacact aactagaata gtagaggctg
     7501 ttgagcaaaa aagaccagtt atatctaaaa atataactgt agttggtcaa ttaaacagtg
     7561 gaaaagaatc tatagtgttt gaagatgtgc caattggaac aactgtcgga gaattaattg
     7621 aaagagctgg tggaataaaa ggtgaatatg gtgaaataat cctaggagga cctttcactg
     7681 gtaaagctac tactttagat gctccaataa ctaaaactag tggtggtata atagttacta
     7741 tgccatttgt taatgaaaag agaaaaatgg gtcttttagt ttgtgcatgt ggacctaatg
     7801 aagaaagaat gagagatata gcaactaaaa tgggagttac tgatatagta tcagttcaaa
     7861 aatgtaaaca agctcaagaa attaaaggtg cattaaaatg tgaaaatcca ggacactgtc
     7921 caggacaagc tcaaaaatgt attgaattta agaaagctgg agcagaagtt atattaatag
     7981 gaaactgtac tgactgtagt aacacagtaa tgggttctgc accaaaatta aaattaggtg
     8041 tatatcatat tactgaccat gtaatgagaa cagttaatca cccattaata agaagaataa
     8101 aaggtgacgt aagattaaaa ataaaataat agataaatag aagttagaca tctttagaag
     8161 taaaacttaa aagttacttc taaggatgtt ttttaatgta ttttgagggc aatatatata
     8221 ttgtaaaata aatggaaaaa atagacaaaa tatgctagat attgtataat gataataata
     8281 gctattacaa ttaatattgg acaaaaataa tgatactcaa gtatgtggtt tgtattaata
     8341 tgcatattat attaagtcaa ctatccgata gagtaaaagg tttttgtgat atttgaagat
     8401 actctatagt atctttttta ttataaaaac taatttttgt cttttattag ataagtatct
     8461 aggatactaa tttatatttt attgagttgt taaaaaagtg cacttaaaaa gttaaaaaag
     8521 aaaaattaaa aaacctattt atagaaactg tgaagtatat cttaaacctg ggcacttaaa
     8581 agatatatgg agttagtagt gcaacctgct ataaatataa ataggagtaa cttttaattg
     8641 tcaaaaatat ataaaataaa tacataaata agtaattaat agaagataga tataaatata
     8701 tttttgaaat gctccctgca ttcataacga gtgtagagtg gcagatatct gcccaaaact
     8761 cctttaattg ctggaagttc ctaaagctaa ctaaaccaca acataatcat gaaataagga
     8821 taagtgtgat agagtgtgca tttaataagc acttgaggtt acgaaagtag aaaaaattag
     8881 ttagatagca taaggttaaa tcctaagtgc cttgaaaatg gataatcagc aggtaagctt
     8941 cgaaaagaag aaacttcaac gactagagag aaatctcgta cctacaagtg tggggaaatg
     9001 gggagtacct aatttatggt taggtaatga tatagtctgt gcttaataga aatattaaga
     9061 agttcatagg agaactggtt aggagtaacg accctaattg aacacactaa agatgtatta
     9121 ttgaataaga ttcatataat atttaataat tttaattata ttaagtgaat acaatatatg
     9181 tttttagtat caggagaatt ttgaaaacaa aaattaaaaa atcaagtata atcagtttag
     9241 taattgcttt tagtatggtg tttactgcat ttacaccaat agtatcttat gctgacgaag
     9301 tgactagcaa tgatacaata cttaatggag aagaacaatc aaatggtcag acaccagatg
     9361 tagagaaacc atcagacggt caggtaccag atggggagaa accatcagac agtcaagtac
     9421 cagatgggga gaaaccatca gacggtcagg taccagatgg ggagaaacca tcagatggtc
     9481 agataccaga tggggagaaa ccatcagatg gtcagatacc agatggagag aaaccatcag
     9541 atggtcagat accagatgga gagaaaccat cagatggtca gataccagat ggggagaaac
     9601 catcagatgg tcagatgcca gatggagaga agacatcaga tagtcagacg ccagatgtag
     9661 agaaaccatc agatggtcag atgccagatg gggagaaacc attagatgag caaactccag
     9721 aagaagaaaa accattggaa gaagaaatag ttatagagga aatgagttta aaacaagata
     9781 ttgataaaat tcttgatatg actttatctc aaataaataa aatagtatat aacttttggg
     9841 aagatgaaga agatgtaaag gcagatgaac aatcagaaat taaccaagtt tttacttcag
     9901 aagactcatt tattagctta tggtatgaca aaaaagcaaa agtaaaaaat tcttgtttac
     9961 taaaagaaat agatggttca gatagacctt atcatgattt aagatttgat gatattacaa
    10021 agactttaac ttttgactat gtaataaagg gtcttgtagg agcaagctca aaagacgaca
    10081 aatatgtaat agaaggtgaa gatggagagc aaactgcatt ttgttacaat aatcatttaa
    10141 gaccaccaag tagtaatgga aagagtccat atttaccagc agaagatttt aatggtcaag
    10201 aaaaccaaaa tgaagaagca gttaaatcta tactatatgc aggttctgaa tttgatggat
    10261 ttggatataa gcaacaattt aatttaggtg gagaagaaaa tgaattgatg acttactcag
    10321 ctactcaaag tgcaatttgg attattcttg gacaaatgga tgaacaagaa agacttaagc
    10381 agtatcaagg ttctataaac ctttgtgata aacttataga aagagcaaaa actgaagagg
    10441 aaagagctga gtatcagaag aagaaagaag tagctataaa tataaaagct tatttagaag
    10501 cattgttaaa agcaggtcgt gaagagttaa agccaaatga tacaggaaaa cctagtttga
    10561 gtaatggttt aactaagatt aactttgaaa aaaatgaaga tggtacttat gaaacagaag
    10621 ctgtagcctt agtaggttat agtggtgtag taaagttaag actaccagat ggagtaactg
    10681 cttatgatgg agatggtaat ataataggta caggtgaagt agaaatttca acacaacaga
    10741 aatttaaact taagagtgtt gtaaaaccag atgccaaagc taatatttct gctgtatctt
    10801 atgattatat ttttccaaaa gctattcaat attataaggc agtgcttgat ttaggacaaa
    10861 aagaccattc atcttcatta ccatctagta agcaaaattt attaagttat acaatagaga
    10921 aaaaaaatgg tcaagaagtt aactttaata taggtctacc aacagatgat gttgtaaatc
    10981 caccagtgcc accaacagat gatacaaatg gacataagcc aaaaccatca ccaccaatag
    11041 atgatacagt aataaatcca ccagtaccac caatggatga tacaataata aatccaccag
    11101 taccaccaac ggaggatgca gtgttaaatc caccagtgcc accaacggag gatgcagtgc
    11161 taaatccacc agtgccacca acagaggata cagtgttaaa tccaccagta ccatcagtaa
    11221 gtgatacagt ggaaaaaact ccagaactat caagagatga tacaataata aaatcaccaa
    11281 aaacaggtga tgaaactcag ctaatgtcat atgtatttat tagtgtcatt gcaatttgtg
    11341 gattagcgta tcaatgtaaa ataaaaagaa attaatctag attattatta agtttaaaat
    11401 aaaaagtctc aaattgattt ataagtcatt ttggggcttt tttatgtaaa tataatatgc
    11461 aaatatagtt tgtttaaaaa tatatatttt aattatattc ttaaatttat gattggatac
    11521 aggcattcat taattgtttg atgaatagaa aaatgttttg aaggtgaaaa tagtattgaa
    11581 aaaataatat actaagggta taattcattt gatttcattt gtataaattt aattataaat
    11641 tttaaagtga ttatataaat ttaaatgcta attacttaat aaattttaat aataattatt
    11701 taataaataa ttattataat atgctagaat aaatatatat aaataagttt tgatgaggta
    11761 ctactataaa ggggggactt tatgttttcg ataccagaag taaaaaaagt agaagaggta
    11821 atggatacaa aatttacaac tatagacgaa gacacaagaa ttgaagatgc aataaaagaa
    11881 atgataaaaa gcaacacaaa aactctaatg gtaatagatt catctgacca attaaaaggc
    11941 attatatcca tgacggatat acataatctt tatgagatgc ataaaaagta tgaaggtcaa
    12001 ccagtaaaac ttataatgaa gaaggatgta atatatgtca acgaaggttt aaccttagat
    12061 gaatgtagag atataatgat attaaaaaat atagggattc ttcctgttct tagagataat
    12121 aaaattattg gagttctaaa acaagagcac attagggact atctatatat gcatttagaa
    12181 gattatggat taactttgaa gtatattata ggtcaaatta aagaaggaat ttgtgctata
    12241 aataacgaag gtgttgttat cttgtggaat aagtttatgg aggaaaggta tgatataaag
    12301 tctgaagata tagttggtcg accgatgaat gaatttttgg aaaatactat ttcagaaaaa
    12361 gtactaaata gtaaagttgg aatgagtgac ttgtatttta cagataaaaa agaaaatatg
    12421 tatgcattag ttcatgcaaa tcctatattt tataaagaag aatttatcgg tgttgtatgt
    12481 acagaagttg atgttacaga agctaagata ttagctcttg aacttgaaaa agtaaatgat
    12541 acattaaaat atctaaagaa cgaagtcaaa aatttatcta aaggtagttt tgataagatt
    12601 ttaggtaaaa gttataaatt ggaaaaatcg aaagcgattg caaaacaagt ggctagaaca
    12661 aatagtagca tatttatttg gggcgaaagt ggtacaggta aagaagtatt tgcaagagct
    12721 attcatgatt atagtgagag aaaaggtcaa tttatacctg ttaactgtag tgccatacca
    12781 aatgagttat ttgaaagtga gttttttggg tatgaatcag gtgcgtttac aggagcaagt
    12841 aaaaagggtc gaataggtat atttgaactt gcaaaagatg gaactgtatt tttagatgaa
    12901 attgccgatt tacctcttag tatgcaagca aagttgctta gagtattaca ggaaaaagaa
    12961 ataagaagag ttggtggaga taccacaata aaaataaatc ctagaataat atcagctacc
    13021 aataaagatt tgagtaaaat ggtaaaggca gaaaaattta gagaagattt atactataga
    13081 ttaaatgttg ttgaaataaa gattcctcca cttagagaga gaaaagaaga cataggatta
    13141 ttggttcata gctttttaga ggagatatgt aaacaaaata ataagcctgt tcttacaatt
    13201 agcaaagatg taatagatat atttcagaat tatagatgga aaggtaatat tagagaatta
    13261 aaaaatacga ttgaaaatat tgttgtattg tcacaaaatt ctaaaataga tgtggatgat
    13321 gtaccaagtt atatgatgga ttctacaaat aatagtactg aagaagaaga gtatccttta
    13381 gatttaacga aggcaactca aaaaattgaa ataaagaata taaccaaggc acttaaaatg
    13441 tctaatggaa ataaagctaa agcagctaag atattaaata tacccagaac aactttgtat
    13501 tataagatag accaatacaa aatagatgtg tctaaaatat gacacatgac taaattttga
    13561 caaaatgata aactttaatt taaaacctag taaagccaac gcttatataa gtgttgtgta
    13621 tatagatgtg ttaaaaaaaa tgaaaaagtg ttgattaatt gacacttttt ttgtttgaga
    13681 aaatgtttac aaatacaaaa aataacatat tttaaataaa tatttatata atatttggct
    13741 aaaaatagcg atagatgaag aatctattat agaaaaaatc tttttgttaa taagttggca
    13801 taggaattgc ttataatata aagtgacttt aagtgagaaa aaaataatat taatcataac
    13861 aaattatgta ggttttaatt tacttaattt ctcagttaag gggttacctg ttgaggaatt
    13921 gagatttaaa ataataaata agaaaataat gtaaggaggt attttatact atgtcaataa
    13981 ctttagaaac agctcaagcc catgcaaatg acccagcagt ttgttgttgt agatttgaag
    14041 cgggaacaat tatagcgcca gaaaacttag aagatccagc aatatttgca gacttagagg
    14101 attctggatt attaacaata ccagaaaatg gattaactat aggtcaagta ctaggagcta
    14161 agttaaaaga aactttagat gcactttctc caatgactac agataacgta gaaggataca
    14221 aagcaggaga ggctaaagaa gaagtagtag aagaaacagt agaagaagca gctccagtat
    14281 cagaagcagc agtagttcca gtaagcacag gagttgcagg tgaaacagtt aaaatacaca
    14341 taggtgaagg taagaacata agcttagaga tacctttatc agtagctggt caagcaggag
    14401 ttgctgctcc agtagcaaac gttgctgctc cagtggcaag tgcagcagca gaagtagctc
    14461 caaaagttga agaaaagaaa cttttaagaa gcttaactaa aaaacacttt aaaatagata
    14521 aagttgaatt tgctgatgaa actaaaatag aaggaactac tttatacatc agaaacgcag
    14581 aagaaatatg taaagaagct aatgaaactc aagagttagt tgtagatatg aagttagaaa
    14641 taataactcc tgataaatat gaaacttaca gtgaagctgt attagatata caaccaatcg
    14701 ctactaaaga agaaggcgaa ttaggttcag gtataactag agttatagat ggagctgtaa
    14761 tggtattaac tggtacagat gaagatggag ttcaaatagg tgaatttggt tcttcagaag
    14821 gtgagttaaa tactactata atgtggggta gaccaggtgc tgctgacaaa ggtgaaatat
    14881 tcatcaaagg tcaagtaaca ataaaagcag gaactaacat ggaaagacca ggacctttag
    14941 ctgctcaccg tgcatttgac tatgtaactc aagaaataag agaagcatta aagaaagttg
    15001 acaactcttt agtagttgat gaagaagtaa tagagcaata cagaagagaa ggtaaaaaga
    15061 aagttgttgt tataaaagaa ataatgggac aaggtgcaat gcatgataac ctaatattac
    15121 cagttgagcc agttggtaca ttaggagctc aaccaaacgt tgacttagga aacatgccag
    15181 ttgtattatc tccacttgaa gtattagatg gtggtatcca tgcattaact tgtataggac
    15241 ctgcatcaaa agaaatgtca agacattact ggagagagcc attagtaata agagctatgg
    15301 aagacgaaga aatagattta gtaggtgttg tatttgttgg ttctccacaa gtaaatgctg
    15361 agaaattcta tgtatctaag agattaggta tgttagttga agctatggaa gttgatggag
    15421 ctgtagtaac tactgaaggt ttcggaaaca accatataga tttcgcatct cacatagagc
    15481 aaataggtat gagaggtata ccagtagttg gtgtaagttt ctcagctgtt caaggtgctc
    15541 tagttgttgg taataaatac atgactcaca tggtagacaa caataagtct aagcaaggta
    15601 tagagaatga aatattatct aacaacactt tagctccaga agatgctgtt agaataatgg
    15661 ctatgcttaa aaatgctata gaaggtgtag aagttaaagc tcctgaaaga aaatggaatc
    15721 caaatgttaa attaaataac atagaagcta tagaaaaagt tacaggagaa aaaatagtat
    15781 tagaagagaa tgagcaatct ctaccaatga gtaagaagag aagagaaata tacgaaaaag
    15841 acgaaaacta aaatttagat actaactaag atttagacaa taacataaat ttagatccaa
    15901 gtttaaaaca gtatagagag gtagaattat aatggatagg ttaaaatata tctcatcaga
    15961 gactttttat gaaggtatta tagttgacat caaaggcggt ggagtgacta tagacctaaa
    16021 aggtagactt ggacaattta aaatacctaa tagaatgctt ataactgact acgaacttaa
    16081 gataggtcaa gaagttggat tcatgctaag ctatcctgag gtattaagtc cagagcctaa
    16141 tcaagagtat gtagaaaata ttaggagaga aaaagaaaaa caagcagaaa tgcaatcaaa
    16201 acaagaagaa aaataataaa taatcgtttg aaaatagaag ggagaggaaa tatatgagcc
    16261 ttacaacagt acaaggactt caatctgaaa tattcgttcc aataacacct cctccagttt
    16321 ggactcctgt aactaaagaa ttaaaagata tgactatagc tttagctaca gcagctggtg
    16381 ttcatttaaa atctgacaag agatttaatc tagcaggaga tactacattt agagctatac
    16441 ctaatacagc cacagttgat gaaatgatgg tatcacatgg tggatatgac aatggagatg
    16501 taaacaaaga tataaactgt atgttcccta tagatagatt acatgaatta gctgcagaag
    16561 gatttatcaa aggagtagct cctatgcact atgcattcat gggtggtgga ggaaaccaac
    16621 atgtcttcac tgaagaaact ggtcctgcta tcgctgctaa acttaaggaa gagggagtag
    16681 acggtgtagt tatgacagct ggctgaggta cttgccatag aactgccgtg atcgtgcaga
    16741 gagcaataga ggaagctgga atacctacaa taataatagc agctcttcct ccagtagtta
    16801 gacaaaacgg aactcctaga gcagttgctc cattagttcc aatgggtgct aatgctggtg
    16861 aaccacataa tatagaaatg caaacacata tattaagaga tacattagag caattagttg
    16921 caataccatc tgctggtaag atagttccat taccatacga atataaagct cacgtttaaa
    16981 atgtaattta tatttgaaaa tatagaaccc ttctcctaca cggttaggag aggggtttta
    17041 agaacaaagg tggatattat ggaagaaaaa ataatgagaa gacttgttat aaaaccgttt
    17101 catatgaatg aagttaattt cggttcaaaa acttctataa agaaagatgt acttacaata
    17161 gatttatcaa gtatagatga aataaaagaa agagaagagt taataacaga cataaaagta
    17221 gacattataa aacctggaga ttatgacaga gaaattaata ctattatgga cattatacca
    17281 atatcaacaa aagttttagg cagactggga gagggtatta cacatacttt aacgggtgta
    17341 tatgtaatgt taactggtgc agatgaagat ggaaatcaaa tgcatgaatt tggttcttca
    17401 gaaggaattt tgaaagacca aatggtattt gggagatatg gaactccatc agttgaagat
    17461 tatataatcc atgtagatgt aactttaaag ggaggtttac cttttgaaag aacacttcca
    17521 cttgcagcat tcagagcatg tgatgatttt atacaagaaa taagagcgtc tttgaaaatg
    17581 gaagacggaa gaaatgctac tcaagttcgt gaatattttg acaaaattcg accaaatgca
    17641 aaaaaggttg tcatagtaaa acaaatagca ggtcaaggtg ctatgtatga caatcagtta
    17701 ttctcaaagg aacctagtgg atttgaagga ggaacatcaa ttatagatat gggaaatgta
    17761 cctatgatta tatctcctaa tgaatacaga gatggtgccc ttagagctat gacttaagag
    17821 aggtggatat tatgggaata ggaccttcaa caaaagaaac atctcttcat cactttagag
    17881 acccactttt agacatagtt gaaagtgata aggatgtaga tttacttgga gtaatagtag
    17941 taggaactcc agatggaaat gaaaataaaa cctttgtggg acaaagaacg gccgcttggc
    18001 tggaagccat gagagtagat ggagctatag tttcatcaga tggttgggga aactcacatg
    18061 ttgactacgc taacacattt gaggaaatag gaaaaagaga tatacctgta gttggagtta
    18121 catttaatgg gactcaagct aagtttgttg taagcaatca atatatggat accatagttg
    18181 atatgaacaa atcaaaagaa ggtattgaga cagaagtagt tggagaaaac aacacaaatg
    18241 aaatagatgc taaaaaagct ttggcatttt taaaacttaa aatgagaaaa catggataaa
    18301 aaatttaagt aagggaatga gttttgtggg aatttctaca tcaatgaaaa acacatcact
    18361 tcatatattt agagacccgt tactagattt gatagataat gattctgaca tagacttgtt
    18421 aggagtggtt gtagttggga catcagagca caatgaatgg aaaacatttt tagcaactag
    18481 acttggaaga tggattgaat cattgcgacc tgatggagtg attataactt tagatatggc
    18541 aggaaaccag catattgatt ttacaaatgc tatagcagag tttgtaaaat cagatatacc
    18601 tacagtaggt cttactatta tgggagcaga tggattagta attaccaatc catatttgga
    18661 taaagctacc ataattgatt ataaaaagac aacaggctac atagaaacag aagtagttgg
    18721 agacaatcac atggatgaag ttgatgttaa aaaagcattg gctttcctaa aattaaaaat
    18781 gcgtaaagac gcaaaataaa cctaaggggg aatttgatat gaaatttagc agaagtatac
    18841 aagctataga ctctcataca gcaggtgaag cgactagaat agttgtaggt ggaataccta
    18901 acataaaagg aaattctatg cctgagaaaa aagaatattt agaggaaaat ctagattatt
    18961 taagaactgc tataatgtta gagccaagag gacataatga tatgtttggt tctgtaatga
    19021 ctcaaccttg ttgcccagat gctgactttg gaataatctt catggatggt ggcggatacc
    19081 ttaacatgtg tggccatggt acaataggag ctatgacagc agctatagaa acaggtgtag
    19141 ttccagcagt agagcctgta actcatgtag ttatggaagc tccagcagga ataataagag
    19201 gagacgttac agttgtagat ggaaaagcta aagaagtatc attcttaaac gtaccagctt
    19261 tcttatataa agaaggtgtt gaagttgact taccaggtgt tggaactgtt aaatttgaca
    19321 tatcatttgg tggaagcttc tttgctataa tacatgcaag tcaattaggt ttaaaaatcg
    19381 aacctcaaaa tgctggtaaa ttaactgaac tagctatgaa acttagagat ataataaatg
    19441 agaaaataga aatacaacat ccaactttag ctcatataaa aactgtagac ttagttgaaa
    19501 tatatgatga gccaactcat ccagaagcta cttacaaaaa tgtagttata tttggtcaag
    19561 gtcaagttga cagatctcca tgtggaactg gaacaagtgc taaattagct actttacatg
    19621 ctaaaggtga attaaaagta ggagaaaaat tcgtatatga aagtatatta ggaactttat
    19681 tcaaaggtga aatagttgaa gaaactaaag ttgcagattt caatgctgta gtacctaaaa
    19741 taactggttc tgcttatata actggattta atcattttgt aatagatgaa gaagacccac
    19801 ttaaacatgg atttattctt aaataagtaa taaataaatt aggaaattaa aatagaatag
    19861 tagccacttg tcggttgcta ttctatagct tttaggctat aagattcaga atatatgaaa
    19921 aaataaaaaa ataggaggat ggattatggt agcaatctta aaggcattat atgtagtatt
    19981 cgcagcaatc tttgcagttg tgtttggtaa agacgtagct aaagcaaaca aagaggggaa
    20041 actagaagaa caaagtactc caaaagttgc tgtaacagga tttataacag acttctttga
    20101 tacattagga atagggtcat ttgctccaac aatagcaatg tcaaaggctt taaagttaaa
    20161 tataccagat aaaaaaatgc ctggtacatt aaacgtagca catactattc cagttgttac
    20221 agaagcattc atatttacat caattatccc agtagatggt gttactttag tatctatggt
    20281 tgtggcagca gcagttggtt catatatagg tgcaggaata atagctaaga tggacgagag
    20341 aaaaatacaa cttgttatgg gtgtaacttt agcattaaca gcaatattaa tgttattagg
    20401 tcatccttgg attaacgtat taccaggtgg aggaaatgaa ttaggtctta ctggtgctaa
    20461 attagtaata ggtgtaatag gtaactttat actaggagct ttaatgacag ctggtatagg
    20521 tctatatgct ccaggtatgg caatggttta tttcttagga atgtctgcaa aagttgcatt
    20581 cccaatagtt atgggttctt gtgcattatt aatgccagtt gcaagtatga aattcataaa
    20641 agaagatgca tatactaaaa aagcatcagt tataatagct atatgtggtt taataggagt
    20701 tttcgtagct gcatatatag taaaaactct accaatggat atattaaaag cattagtaat
    20761 agtagttata gcttatactt ctgctacaat gataatggct gcaaataaaa acaaaaagac
    20821 tgcttagtta aagactactt agtgtttatt aaatattaca gctaataata aagattataa
    20881 actgctttaa aatattgaat taatatttta gggcagtttt ttatttacta aattaatata
    20941 aagcaattaa catgccaatt atgtaatata tatagtgatt tagtagattt aatctaaaaa
    21001 acgttgtatt tttaaagaat aaagcataat ttaaagctaa atatttaaat tttataatca
    21061 ataagttcaa taaatgttca tatattaata cagattaata aaaatataac ctgtaataag
    21121 tctaatataa cttataatta agaaaaataa gtttataatg actcaatatt catctgaatt
    21181 atttcaataa atttaattcg ttgaagttgc ttatatagag tgaataaatt taagttatat
    21241 atgacttatt aattatataa taggattttt ataattaata caaaactga
//
LOCUS       DAMJYR010000059        20295 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.059, whole genome shotgun sequence.
ACCESSION   DAMJYR010000059 DAMJYR010000000
VERSION     DAMJYR010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 20295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20295
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.059"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(45..923)
                     /locus_tag="PC471_002176"
     CDS             complement(45..923)
                     /locus_tag="PC471_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HDF2789304.1"
                     /translation="MRYDIAIIGSGPAGLSAAINAKIRNKTIIMFGNDNLSNKLVKAP
                     SIDNYLGFYDISGDELKDKFKSHIDSMDISIENKRINNIYAMGEYFTIMSGNDMYEAT
                     TVILATGVEYTRPIKGEEEFLGRGVGYCATCDAPLYRNKKVAVIGYNEESKEEANFLS
                     ELTSKTYFIPMYKKDNLMRSSDNLDDSIEVIHDRPVQIDGDKLVNKVSFKENHIEVDG
                     VFVIKDSTAPSALVPGIEIDGIHIKVDNNMKTSIDGCFAAGDCVGKPYSYIKAAGQGQ
                     IAALNAVYYLDKLKRA"
     gene            complement(940..1257)
                     /gene="trxA"
                     /locus_tag="PC471_002177"
     CDS             complement(940..1257)
                     /gene="trxA"
                     /locus_tag="PC471_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HDF2789305.1"
                     /translation="MAKVINTSEFRSSVEGSKGVVVVDFFATWCGPCNMLGPVFAELG
                     EEMKDKARFVKVDIDESLEIAQQFNVSTVPTMIIFKDGKPVETLIGFMPKNKIEMQVK
                     SYL"
     gene            complement(1407..2639)
                     /locus_tag="PC471_002178"
     CDS             complement(1407..2639)
                     /locus_tag="PC471_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF2789306.1"
                     /translation="MINKNVFTSTKNHLIKMYIIVVGSFLIIFSIFIYSYFRGLTYSG
                     IDSEINDELEYIVSQFKRTSFLNPIRLKDPKDMVYVYEDGRISYYTQNEYFDELLPDR
                     RLDKKNSFFKYTENGYTFRELNVDVGRYQIQIIRNIDSEMNSLRQLTSVLIIGILISV
                     IITYFVAVYLTRKALIPIETAWKNQAKFIQDASHELRTPITIVSSKLESMLKSPESTV
                     NDEVETIATAMKETRRLKKMITDLLSLTKEDSIVKVNLEEIDLEKLLEEISEDYIDIA
                     EFQEKRFVFNSKLKNKVIITDKNKLRQLILIFIDNAFKYTKLGDEIALELKEDIEDEV
                     TLLISDTGIGIKKEEIPLIFDRFFRSENVRNKDLEGSGIGLSIARMISLNLSIDINVT
                     SDVDIGTTFELSIPKKLK"
     gene            complement(2629..3300)
                     /locus_tag="PC471_002179"
     CDS             complement(2629..3300)
                     /locus_tag="PC471_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2789307.1"
                     /translation="MKVLIVEDNKILLESVVEELSKHFETEKCEDGEEALYLINQNIY
                     DLVILDLMLPNINGFDILKKMRINNIDTPVLILTAKETLDDKVEAFTIGANDYLTKPF
                     YMEELVARVYAILRTNGKIKERNGLEFKSLYLDTLEKRVYIEKEEIKLQNKQFNLLEY
                     FVLNKGSILLKEQIYDRIWGIDSDATIEIVEVYVSNLRKKLSKYGYDKYIKTKRKVGY
                     IFDDK"
     gene            complement(3316..4152)
                     /locus_tag="PC471_002180"
     CDS             complement(3316..4152)
                     /locus_tag="PC471_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2789308.1"
                     /translation="MNKIVKKSISLMIILTIFIFMLTACEKDEQPDSVDIQTEDKNEI
                     KIDEGNAKVLNIGKSEIINIDEGDKVDTSTKVENNSTFNISNVELIYNEYDENKKIIS
                     SDSKALLDMTLMPGKVAYIECGHKTFVKGVEVYAYEYEAEGKIVYVNLKENTINIRNN
                     NIKLENSSQYEVLSTSELKKVNESNEGITYQVKVKNSSSKDLGNIILKTAEVNENGEY
                     LTVNRVPSYKILKPSEETDIDILCSTKAKSVEIVGYTYDDIKEKANVDIDLKSHKVKI
                     DK"
     gene            complement(4178..4852)
                     /locus_tag="PC471_002181"
     CDS             complement(4178..4852)
                     /locus_tag="PC471_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HDF2789309.1"
                     /translation="MNNNEDRIDKFLELQSKGFKFAEIAQNLKISQSTLRTFLNKKGY
                     KSEKGKYILKDELNYNKSLDNRNLEEEKAKEYEKSKKNTISNRKKVSTKKKISNEKAL
                     SKSVEQIEFNDTGLKNAKPKQNIKPKKDRKINITQEDLDKLCEVYDWYLEVKDYNIKP
                     KKVSNKKDVHIDDEHIDELKTTRIRIDKEIWNDFERLCSNSNFEKHQILTQALKEFMK
                     KYKNLL"
     gene            complement(4866..5594)
                     /locus_tag="PC471_002182"
     CDS             complement(4866..5594)
                     /locus_tag="PC471_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HDF2789310.1"
                     /translation="MMFDRGKWNKLDYNELLEYMMSIQDIKYRDFNKKLIPGTENIIG
                     IRVPNLRKLSKEISQGNWKEFLEVAQDTYYEEVRLQGMVIGNINSNFEETLYYVKRFI
                     PKIDNWSVCDGFCSDLKSMKKYKENMYDILKKYVYSKNPWEIRFALVMFLIYYVDDKH
                     IKEIFEYCNNIQSEEYYVKMGMAWLLSICFIKCEEETFLYIKNNNLDDFTYNKMLQKI
                     IESNRVDLEKKNIIRSMKRKTRRC"
     gene            complement(5721..6410)
                     /locus_tag="PC471_002183"
     CDS             complement(5721..6410)
                     /locus_tag="PC471_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HDF2789311.1"
                     /translation="MDRYSEITNKNQREIVLLKGFPCIWGKCSFCDYIDDNSNLEEEM
                     NKLNLKVLKNVTGKYGVLEVINSGSCFELPKDTLEKIKCIIKEKNIKKLFLESHWSYK
                     NRLKEMREYFEIPVVFKIGVETFDNDFRNNILNKNANFKTPQDVKEYFDSPCIMVGIK
                     GQTKEMIDKDIEIILNTFEKATVNVFINNSSSIKRDEELVKWFVSKYKFLDENPNIEV
                     LYNNTDFGVGD"
     gene            complement(6418..6981)
                     /locus_tag="PC471_002184"
     CDS             complement(6418..6981)
                     /locus_tag="PC471_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HDF2789312.1"
                     /translation="MKRKICVKTITLIAFGISINIIGAFIAMGLRLPVYLDSIGTIMI
                     ASLLGPKYAVVTGVFGSLISGITFDVYSLYFAPVQISTGLLAGIAFKKNFLRGLKTPL
                     GVLLFAIPTSIISSIISAFLFGGMTSSGSSYIVQILKVLGLGDVFSVFVTQVFTDYGD
                     KLLAVVLVNLGLNAVPKTLKVSLTGGK"
     gene            complement(6983..7951)
                     /locus_tag="PC471_002185"
     CDS             complement(6983..7951)
                     /locus_tag="PC471_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HDF2789313.1"
                     /translation="MEKRKVIIDCDPGIDDSLAILLALNSPELEVIGITTCCGNVPAN
                     IGAENALKTLQMCSSLNIPVYIGEEAPLKRKLVTAQDTHGEDGIGENFYQKVVGAKAK
                     NGAVDFIINTLYNHEKVSIIALAPLTNIAKALIKDKKAFENLDEFVSMGGAFRIHGNC
                     SPVAEFNYWVDPHGADYVYKNLSKKIHMVGLDVTRKIVLTPNIIEFINRLDKKMAKYI
                     TEITRFYIDFHWEQEGIIGCVINDPLAVAYFIDRSICKGFESYVEVVEDGIAMGQSIV
                     DSFNFYKKNPNAIVLNEVDEKKFMYMFLKRLFKGYEDIIDSVEGVI"
     regulatory      complement(8052..8095)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            8461..9090
                     /locus_tag="PC471_002186"
     CDS             8461..9090
                     /locus_tag="PC471_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDF2789314.1"
                     /translation="MSIKERRKNEKEEMKKKIMDASIEIINQHGYENLSIRKIATKIE
                     YSPTTIYLYYKDKAEIISDMTNKLYNTVESNAIDIMNNCSSLPIDKQIIEIMTSFIKT
                     LSSEPEMAKSIMHSRMNIIFASENTNNTPSNNGIKMLDKFLSIGIEQGIFKKNIDKSS
                     WMLISALLGFVLCVVENKLYSLHNFSQLIENFLDILVGGLYNENSQQGY"
     gene            9065..9469
                     /locus_tag="PC471_002187"
     CDS             9065..9469
                     /locus_tag="PC471_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HDF2789315.1"
                     /translation="MKIRNKATNKKKKYVKISIALFFVFICIIIVIVSNLKPENLVVK
                     DIDINNVKNGIYTGSADNNLVKATVSVEVNNGKIQNINILKHDHLLGKPAEKITTSII
                     KQQSLDVDAITSATYSSNTIRKAVENALRKGE"
     gene            9472..9948
                     /locus_tag="PC471_002188"
     CDS             9472..9948
                     /locus_tag="PC471_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin domain-containing protein"
                     /protein_id="HDF2789316.1"
                     /translation="MNTIIIYSSKYGCTKDCANILKNKLSDNVTIVDINNNNNKIELS
                     KFDKIIIGSSIYVGSVSKKIQVLCNDNVELLNKKQVGIFLCCGFSEQADKYLKSNFPS
                     SLLESANAIGIFGSEARLEKMKFLDKLIMKAVSKGNYDSFRISQDNIDNFLINLNS"
     gene            10520..11185
                     /locus_tag="PC471_002189"
     CDS             10520..11185
                     /locus_tag="PC471_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HDF2789317.1"
                     /translation="MIKLIGILIVVIGFILKIDTLFTVLLAGVATGIVAGLDFNQIFT
                     ILGDSFVSNRGVSLFILTLPVIGVLERYGLRQRAVSLIEKLKRLTTGKVLTIYMIARQ
                     IAGALSIRMSGHPQFVRPLVNPMAQAAGLSNSDELKESDEEAIKALSAASENYGNFYG
                     QNLFAGSSGVLLIASTLTQFGYNVTGLNIVKASTIMAVIALVVATIQFTLYDKKLNKS
                     HKK"
     gene            11207..12139
                     /locus_tag="PC471_002190"
     CDS             11207..12139
                     /locus_tag="PC471_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HDF2789318.1"
                     /translation="MQNFINISLEIFYALMGFLMIVIAYKSFTTINNNKKYGTSLFWI
                     LISLPFIFGRLIPANIIGIILILSSLLTLSKQVVFAKYEEPDENFGKEQADKLKNKIF
                     IPSLILAFAAVVVAMSLSNFDNSSQFAIGVGSIIALISALIITKAKPATSVQDGSRLL
                     QQMGPASMLPQLLVALGALFTQAGVGEVISTMISGVVPADSRLFGVIAYVLGMVIFTM
                     IMGNAFAAFSVITAGIGIPFVLSQGGNPAIIGALALTAGYCGTLLTPMAANFNIVPAA
                     LLECKNDYIVIKYQAPVALVLIIAHILVMYFLGF"
     gene            12160..12801
                     /gene="pcp"
                     /locus_tag="PC471_002191"
     CDS             12160..12801
                     /gene="pcp"
                     /locus_tag="PC471_002191"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HDF2789319.1"
                     /translation="MKILLTGFDPFGGEPINPAQEAVERVNNNINGAEIIKITIPTVM
                     TKSVEAIDKAIQEHNPDIVISVGQAGGRFDITPERVAINIDDFRIKDNEGNQVIDTII
                     KEDGEPAYFSKLPVKAMVKHMNENKIPASVSNTAGTFVCNHVMYGILYMIDKKYPNIR
                     GGFIHIPYTTSQVIDKKNTPFMSLEEIVKGLELAIEACIIYKEDVKEIGGEIS"
     gene            complement(12921..13253)
                     /locus_tag="PC471_002192"
     CDS             complement(12921..13253)
                     /locus_tag="PC471_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2789320.1"
                     /translation="MSIDCISNTDLSETGFSYTLSLISGKYKMIILYCLVEFEVVRYN
                     ALKRYINTISYKTLSLSLKELEADNLIIRTEYPQIPPKVEYSLSERGKSLIPILDAMC
                     EWGERNRP"
     gene            13519..14139
                     /locus_tag="PC471_002193"
     CDS             13519..14139
                     /locus_tag="PC471_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HDF2789321.1"
                     /translation="MKVLLINGSPNQYGCTYTALNEITKVLSKHDIKTEILYLGKETI
                     PGCISCASCFETGKCIRNDKVNELIEDLDNIDGIIIGSPVYFSSATGQLTSFLDRLFF
                     IAGSRMATKLGASVVSCRRGGASATFDQLNKYFSISNMPIVSSQYWNQVHGFTPEDVM
                     KDEEGLQTMRTLGENMAWLLKCISAGKKAGIKEPQYEETIMTNFIK"
     gene            complement(14464..15288)
                     /locus_tag="PC471_002194"
     CDS             complement(14464..15288)
                     /locus_tag="PC471_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraX"
                     /protein_id="HDF2789322.1"
                     /translation="MDFFKKGISGFTIKILALIFMTFDHIAAFMPQTMQIPIWFHWVG
                     RISAPLFIFMAVEGFYHTSNRKKYISRLYIWSVIMAIGNQIINNVFSHPEGAIIINNT
                     FSTLFLIAIYLQAIEFIKKFRKEKEIKYFIIGLLMIIIPIILGIFTVALLFKVTNRVI
                     ALFMILVPVPFLVEGGPIWIILGIIFYLCRGKKFSLSICYVLMCIFIFTTMSNGDYSL
                     KNSILQNYQWMMIASLPLMLLYNGEKGKSMKYLFYLYYPIHVYILYILGIYLINGF"
     gene            complement(15299..16702)
                     /locus_tag="PC471_002195"
     CDS             complement(15299..16702)
                     /locus_tag="PC471_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF2789323.1"
                     /translation="MKWKITRNFIFTIVFVAISVVIINIISILYVISTNSFFKVVDSG
                     NNPEEFARSFEKDLYEKDGEFKLSKIGAEKLEKSNSWIQVLNDLGEEVYGVNVPKDTP
                     KKYTPFQMVNNYKYIETKYVNFVLEKYLNKKHLNIIVGIPSRDISRIILTYSQNNIKK
                     TLNKVIIITLVIDSVVALGVGYLFSRKLTKPISSVLWSIETMANGNYSLYLKDRGIYE
                     EVFKNINMLADTLRVNEVERKENEELREEWLANITHDIKTPLASIQGYAEIINDKDYE
                     FEEDEIQEYTEIIYNKSKYIKDLVDDLNLSTRLKNNTIVLDKKKINLVSLVRDIIIDI
                     LNDNRYKNRNIEFESNEDLIEVYVDSILFRRAITNLIFNSIVHNSEGTLISVEIVKKD
                     NIEIIIKDDGIGISKSDLKHIFKKYYRGTNTGEMHKGSGLGMAISKEIIEIHKGKIYV
                     SSEIGIGTKIIIEIKQN"
     gene            complement(16706..17431)
                     /locus_tag="PC471_002196"
     CDS             complement(16706..17431)
                     /locus_tag="PC471_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012450879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2789324.1"
                     /translation="MENRVLIIDDEVEILKLLETVLKKEGLNNIYTAKTKKEGLELFK
                     SINPDLIVLDIMLPDGEGYDICKEIRKTSNSPIIFLSAKTEELDKLLGLAIGGDDYVT
                     KPFSPKEVAFRVKAHLRRLSYFSDAQNESKNLNNNEEKIISFGPYILNESRAELIKDG
                     KSIGLSAKELKILSLFAHNQNQIISKEKLWDKVWGEDYVGFDNTIMVHIRKIREKLED
                     NPSKPEYILTIKGLGYKLAVKED"
     gene            complement(17543..18316)
                     /locus_tag="PC471_002197"
     CDS             complement(17543..18316)
                     /locus_tag="PC471_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2789325.1"
                     /translation="MMYLVKAEMIKILKSKSVWIIWLFLIFFGFFLIRKFDVLDTYAD
                     IFYKIEGSIPLIGLIMFIITSGNYIKEYDSNMTGLINTTKKGKKSVVLSKLVANGLIL
                     SIINISFILLVGIRGFSFFDFKNLNESIHNLWYFGNSNLNLTVIQMYIIVILTTIFAS
                     FIFAQIGLTLSSIFKSAVIPFILGGLIMAIPYFSVGFIPDKAIKFMSVTPNWIMMSQQ
                     MVKYNVPSILIVFSIVISIILMIVLTKITYENFTSSKRF"
     gene            complement(18322..19209)
                     /locus_tag="PC471_002198"
     CDS             complement(18322..19209)
                     /locus_tag="PC471_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011948651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2789326.1"
                     /translation="MNKLEIENLSKIYGKKVANDKITVTLENGVYGLLGPNGAGKSTL
                     MKQITTLIKPDEGQILYNGEDIFKMDADYRNILGYLPQEFGVYKNFTAKEFLQYIGAL
                     KGLRGKYLNSKIGELLDLVGLYDVRNKSIGKFSGGMKRRVGIAQVLLNDPKIIVLDEP
                     TAGLDPQERARFRNLIAKMSRDKIIILSTHIISDIESVAKETIMIKDGKLLMKGTHKD
                     ILSHMENKVYNVQVKNECEVDDIQSKYKVVSIQSDIDSVVLRIVSEDIPSEDNIQPVQ
                     AKFEDVYMFYFDLEDAREV"
     gene            complement(19223..20245)
                     /locus_tag="PC471_002199"
     CDS             complement(19223..20245)
                     /locus_tag="PC471_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2789327.1"
                     /translation="MVNWEIKKLAKSKSMFISLGILVLILMISIFITPVLETESYYID
                     KEKGRIEDTRSGIDVGNEKFQNKINVLKQFSNQTDTGEFSKKIELMSKKKLDNLSVNE
                     YKDISFWKVFNYRVTNSLINVGMLIIISIIISNIYVDEVVTSVKDIILSSKEKKKALK
                     SKIFVALLVPLVIYSVYLMGIFIITCMQQGVPVNGDLESFRIVDNVVALKTNLSITKY
                     VILNIGIELLMFEGWAMIAIFGSFISKSSISSISFFIFIISITKIMSVIHILPKKLLS
                     VVSNVNYYDLIFGFNKIIGNYLGDIMIFKVNIDIVNIAIGLLMAILIGAMSLCFLVIK
                     SDYISR"
BASE COUNT         7011 a         3241 c         2184 g         7859 t
ORIGIN      
        1 acagtttttt ataaatatct ttaaaaagtt aatcaatata aatattaagc cctctttaat
       61 ttatctaaat agtaaacagc atttaaagct gctatttgcc cttgaccagc tgcttttata
      121 taagaatagg gttttccaac acaatctcca gcagcaaagc atccatctat actagtcttc
      181 atattattgt caactttaat atgaatgcca tcaatctcta ttccaggaac caatgcactt
      241 ggagccgtac tatcttttat aacgaatact ccatcaactt caatgtggtt ttctttaaaa
      301 gatactttgt ttacaagttt atctccatct atctgtactg gtctgtcatg tataacttca
      361 atagaatcat ctaggttatc tgaacttctc ataagattat cttttttgta cataggtata
      421 aaataagtct ttgaagtgag ttcacttaaa aaatttgctt cttctttaga ttcttcatta
      481 tatcctataa ctgctacctt tttatttcta taaagtggtg catcacatgt agcacagtag
      541 cccacacctc ttcctagaaa ttcttcttca cccttgattg gtctagtata ctctacccca
      601 gttgcaagga ttacagtagt agcttcatac atatcatttc cagacattat tgtaaagtat
      661 tctcccatag cataaatatt atttattctt ttattttcaa ttgaaatatc catactatct
      721 atatgtgatt taaacttgtc ttttaattca tcaccactta tatcataaaa acctaaataa
      781 ttatctattg aaggagcttt aacaagctta ttacttaaat tatcattacc aaacatgata
      841 attgttttat ttcttatttt tgcatttata gcagcagaca atccagctgg accactgccg
      901 attatagcta tatcatatct cataatactc acccattttt tataaatatg attttacttg
      961 catttcaatt ttgttctttg gcatgaatcc aattaaagtt tctactggtt ttccatcttt
     1021 aaatataatc atagttggta ctgtagatac attgaattgt tgagctattt ctaaactctc
     1081 atctatatca acttttacaa atctagcttt atccttcatc tcttcaccta attctgcaaa
     1141 tacaggtccc aacatgttac aaggtccaca ccaagttgca aagaaatcaa ctacaactac
     1201 tcccttacta ccttcaacac tacttctaaa ttcagatgta tttataactt ttgccatttt
     1261 taaatcctcc tattaaactt tattatccct atataattat actatgaaat ttataaatat
     1321 aaatttactt ttataaggtc tttttatatt ttatactaat tagtataccc attcttcatt
     1381 tctaaaaaca attttttata tttttattat tttaactttt ttggtattga caattcaaat
     1441 gtagttccaa tatctacatc agaagttaca tttatatcaa tagataagtt taaactaatc
     1501 atgcgagcta ttgaaagtcc tattccagaa ccttctaaat ctttatttct tacattttca
     1561 cttctaaaaa atctatcaaa tataagaggt atctcttctt tttttatgcc tataccagta
     1621 tcacttataa gtaaagttac ctcatcctct atatcttctt tcaattctaa tgctatctca
     1681 tcacccaatt ttgtatactt aaaggcatta tcaatgaaaa ttaatataag ttgtcttaat
     1741 ttatttttat ctgttattat aactttattc ttcagtttag aattaaacac gaatcttttt
     1801 tcctgaaact cagcaatgtc aatatagtct tctgatattt cttccaataa cttttctaaa
     1861 tctatttctt ctaaattaac tttaacaata gagtcctctt tagtaagaga caataaatca
     1921 gttatcattt ttttaagtct tctagtttcc ttcattgcag tagctattgt ttcaacttca
     1981 tcatttactg tactttctgg ggattttaac atactttcta attttgatga aactattgtt
     2041 attggtgttc ttaattcatg agaagcatct tgtataaact tagcttgatt tttccatgct
     2101 gtttctattg gaattaaagc ttttctagtg agatatacag ccacaaagta agtaattatt
     2161 acagaaatta atattccaat tataagaaca gatgtaagct gtcttaaaga attcatctca
     2221 gaatctatat ttctaattat ctgtatttgg tatctaccta catctacatt taattctcta
     2281 aaagtatatc cattttctgt gtatttaaaa aacgaatttt ttttatctag tcttctatct
     2341 ggaagtaact catcaaaata ttcattttga gtatagtaac ttatccttcc atcttcatat
     2401 acatacacca tatctttagg atctttgagt cttattgggt tcaaaaatga agttctttta
     2461 aattgagaga ctatatattc aagttcatca tttatctcac tatcaattcc actatatgta
     2521 agtcccctaa aatatgagta tataaatata gaaaagatta ttaaaaaact acctactaca
     2581 ataatataca tttttattaa atgattttta gtactggtaa atacattctt atttatcatc
     2641 gaatatatat cctaccttac gtttagtttt aatatattta tcatatccat atttacttag
     2701 cttttttctt agattactta catatacttc cactatttca atagttgcat ctgaatcaat
     2761 tccccatatt ctatcataaa tctgctcttt taataaaatt gaacctttat ttaatacaaa
     2821 atactcaagc aagttaaact gtttgttttg aagttttatt tcttcttttt caatatacac
     2881 tcttttttct agtgtatcta aatataaaga tttaaattct aatccgtttc tttcttttat
     2941 ctttccatta gtccttaata tagcgtatac cctagctact aactcttcca tataaaaagg
     3001 ttttgtcaaa taatcattag ctcctatagt aaatgcttct actttgtcat ctaatgtttc
     3061 ttttgctgtc aatataagta caggtgtatc tatattatta attctcattt tctttaggat
     3121 atcaaatcca tttatattag gaagcattaa gtctaaaatg actaaatcat atatattttg
     3181 atttattaag tataaagctt cttctccatc ttcacacttt tcagtttcaa aatgcttgct
     3241 aagttcttct actacacttt ctaaaagtat cttattatct tcaactatta aaaccttcat
     3301 aagtatcccc tacctttatt tgtctatctt aactttatga gattttaagt caatatccac
     3361 attggctttt tcttttatat catcatacgt atatcccact atttcaacac tttttgcctt
     3421 tgttgaacac aatatatcta tatctgtctc ttctgaaggc tttaaaatct tatatgatgg
     3481 tacacgattt actgttaaat attctccatt ttcattaact tctgctgtct taagaataat
     3541 atttcctaaa tcttttgatg atgaattttt tacttttact tgatacgtaa ttccctcatt
     3601 tgattcattt acttttttaa gttctgatgt agatagcacc tcatactgac tactattttc
     3661 tagcttaata ttattatttc ttatattaat agtattttct tttaaattaa catacacaat
     3721 cttaccctct gcctcatatt catatgcata aacttctaca ccctttacaa aagttttatg
     3781 cccacattct atgtaagcaa ctttcccagg cattaaagtc atgtccaaca aagccttact
     3841 atccgaagat ataatttttt tattctcatc atattcatta tatatcaatt caacattgga
     3901 tatattaaat gttgaattat tttctacctt tgttgatgta tctactttat caccttcatc
     3961 tatatttata atttctgatt ttcctatatt caatactttt gcatttccct catctatttt
     4021 tatctcattt ttatcttctg tttgaatatc aactgaatca ggctgttcat ctttttcaca
     4081 tgctgtcaac ataaaaataa atattgttaa tataatcatt agagaaattg atttttttac
     4141 tattttattc atattaagca cctcatatag tagtatttta tagtagattt ttatactttt
     4201 tcataaattc tttcaatgcc tgtgtaagta tttgatgttt ttcaaaattc gaattactac
     4261 ataatctttc aaaatcattc catatttctt tatctatacg tattcttgtt gtttttaatt
     4321 catctatgtg ttcatcatct atatgtacat cttttttatt agatactttt ttaggcttta
     4381 tattgtaatc ttttacttct aaataccaat catatacttc acaaagctta tctaaatctt
     4441 cttgagttat atttattttt ctatctttct taggcttaat attttgtttt ggctttgcat
     4501 tttttaaacc tgtatcatta aactcaattt gttcaacact tttacttaaa gccttttcat
     4561 tagaaatctt ttttttggtg cttacttttt ttctattact tatagtattc tttttacttt
     4621 tttcatactc cttagctttc tcttcttcca agtttctatt atccaaactt ttattataat
     4681 ttaattcgtc ttttaatatg tatttacctt tttctgactt atatcctttt ttatttaaaa
     4741 aagttcttaa agtactttga ctaatcttta gattttgagc tatctctgca aatttaaatc
     4801 ctttgctttg aagttctaaa aatttgtcaa ttctgtcttc attgttattc attaatattc
     4861 ctccgtcaac atcttcttgt ctttcttttc atacttctta ttatattctt cttttctaaa
     4921 tctactctat ttgattcaat tattttttgt agcattttat tataagtgaa atcatctaaa
     4981 ttattatttt ttatatagag aaaagtttcc tcttcacatt taataaaaca aattgatagc
     5041 aaccatgcca tacccatttt aacataatat tcttcacttt gtatattatt acagtattca
     5101 aatatttctt taatatgttt atcatcaaca taatatatta aaaacataac taatgcaaat
     5161 ctaatttccc atggattctt tgaataaaca tattttttaa gtatatcata catattttct
     5221 ttatattttt tcatagactt taaatcagaa caaaatccat cgcaaactga ccaattatcg
     5281 attttaggta taaatctttt tacatagtat aaagtttctt caaaattact atttatattg
     5341 ccaattacca taccttgtag cctaacttct tcatagtatg tatcctgtgc tacttctaaa
     5401 aattccttcc aattcccttg gcttatttct tttgataact ttcttaaatt aggaactcta
     5461 atacctatta tattttctgt tccaggtata agctttttat taaaatctct atattttata
     5521 tcttgtatag acatcatata ttctaaaagt tcattataat ccaatttatt ccattttccc
     5581 ctatcaaaca tcatttaatt gctccttaat tccttttttc ttattgtaac ataaaactct
     5641 acaaaaaaag cttctaacta ttagaagctt tttcaaaaac ataagaaact tttcaatttt
     5701 aaatttaaac taaattaatg ctaatctcct acaccaaagt ctgtattatt gtataaaact
     5761 tctatatttg gattttcatc caagaattta tacttactaa caaaccattt cactaactct
     5821 tcatctcttt ttatactaga agaattattt ataaaaacat tcactgtcgc tttttcaaaa
     5881 gtatttagga taatctctat atctttatct atcatctctt ttgtttgtcc ttttattcca
     5941 accataatac aaggtgaatc aaaatattct tttacatctt gtggagtctt aaaatttgca
     6001 tttttattta aaatattatt tctaaaatca ttgtcaaagg tttcaactcc aattttaaat
     6061 accacaggaa tttcaaaata ctctctcatt tccttaagcc tatttttgta agaccaatga
     6121 ctttctaaaa ataatttttt tatattcttc tcttttataa tacactttat tttttctaaa
     6181 gtatccttag gtaattcaaa acagcttcca gaatttatga cttcaagaac tccatatttc
     6241 ccagttacgt tttttaaaac ctttagattt aatttattca tctcttcttc taaattagaa
     6301 ttatcatcta tataatcaca aaaagaacac tttccccata tacaaggaaa tcctttaagc
     6361 agaacaattt ctctttgatt tttatttgtt atctcactat atctatccat tttctatcta
     6421 ttttcctcca gtcaatgata cctttaaagt ttttggaact gcatttaatc ctaaattcac
     6481 taaaacaact gctaacagct tatctccata atcagtaaat acttgggtta caaacacact
     6541 aaaaacatcc cccagtccta acactttcaa gatttgcact atatatgatg aacctgaaga
     6601 ggtcatacct ccaaataaaa atgctgaaat tattgaactt attatagatg ttggaatggc
     6661 aaataaaagt actcctagtg gagtttttaa accccttaaa aaattctttt taaatgctat
     6721 tccagcaagt aatccggttg atatttgaac tggtgcaaaa tagagagaat acacatcaaa
     6781 cgtaattcca cttattaaac ttccaaacac acctgtaaca acagcgtatt taggtcctaa
     6841 aagtgaagct atcattatag ttcctattga atctaagtat acaggtaatc ttaatcccat
     6901 agctataaaa gcgcctatta tatttataga aataccaaaa gctataagtg taatagtttt
     6961 tacacatatt tttcttttca cactatatca ctccttccac agagtctata atgtcttcat
     7021 aacctttaaa aagcctcttt aaaaacatgt acataaattt cttctcatca acttcattta
     7081 gaacaattgc attaggattt tttttataga aattgaaaga atccactata gactgaccca
     7141 tagctattcc atcttctaca acttctacat atgattcaaa tcctttacat atacttctat
     7201 ctataaagta tgctactgct agagggtcat ttatcacaca gccaattatt ccctcctgtt
     7261 cccaatggaa atcaatataa aatctagtta tttcagttat atactttgcc atcttcttat
     7321 caagtctatt tataaactca ataatattag gagtaagtac aatttttcta gttacatcta
     7381 aacctaccat gtggattttt ttagataaat tcttgtaaac ataatctgct ccatgtgggt
     7441 ctacccaata attaaactct gctactggag agcaatttcc atgaatccta aatgctcctc
     7501 ccatagatac aaactcatcg agattttcaa atgctttctt atctttaata agtgctttag
     7561 ctatatttgt aagtggtgca agtgctatta ttgatacttt ttcatgatta tataaagtat
     7621 ttattataaa atccactgct ccattttttg ctttagctcc tacaaccttt tgataaaagt
     7681 tttctccaat accatcttct ccatgtgtat cttgagctgt tacaagtttt ctttttagtg
     7741 gtgcttcttc tcctatatat actggaatat ttagtgaaga acacatttga agtgttttta
     7801 gtgcattttc tgcacctata tttgctggga catttccaca acatgtggta attccaatta
     7861 cttctagctc tggtgagttt aaagccagaa gaattgccaa agaatcatca attcctgggt
     7921 cacaatcaat tattactttt ctcttttcca ttttttatcc tccttgattt gtggtcgagg
     7981 agaactacac atcttttttc cactttcaaa aaggtgtgtc taagggtatt aattatatac
     8041 tgccactata taattcctta gttttttata cttggagttc tcgaacaagt acagatgtaa
     8101 aatcatattt ttaggctact tactataaga ctcttaaaaa ctatagaccg ttaaaaaatt
     8161 tttaaacatc tgatttaata gctataaaat aaattttata gcataaaaga atttattata
     8221 ttttaatgta tcttattaaa gtttatttag aattatgaac caactttaat aagatgtggc
     8281 gcatttaaac cgattattat tatacaataa tatcttaaat attacaatta ataacattaa
     8341 tttattatcc ataaaaatat attaacttat attatttaat aatttattga cagtattaaa
     8401 attaaattat acaataacag tgtaaattaa cttaacaccg ttaagcaaag aggtgattat
     8461 atgagtatca aagaaagacg taaaaatgaa aaagaagaaa tgaagaaaaa gattatggat
     8521 gctagcatcg aaattataaa ccaacatgga tatgaaaatt tatcaataag aaaaattgct
     8581 acaaaaatag aatattctcc aactacaatt tatctttact acaaagacaa agcagagatt
     8641 atcagcgata tgaccaataa actttataac acagtagaaa gtaatgccat tgacattatg
     8701 aataattgct catcactacc aatagataaa caaatcatag aaattatgac tagctttata
     8761 aaaactcttt ctagtgaacc agagatggca aaatctatca tgcatagtag aatgaatatt
     8821 atatttgcat ctgaaaatac aaacaatacg cctagcaata atggtataaa aatgcttgat
     8881 aaattccttt ccattggaat tgaacaagga atattcaaaa aaaatataga taaaagctca
     8941 tggatgctta taagtgcatt gttaggattc gttttatgtg ttgttgaaaa taaactatat
     9001 tctttacata atttttctca acttatagaa aatttcttag atattttagt gggaggactt
     9061 tacaatgaaa attcgcaaca aggctactaa taaaaagaaa aaatatgtaa aaatatctat
     9121 tgctctattc tttgtattta tatgcattat tattgtaatt gtaagcaact taaagccaga
     9181 aaacttggta gtaaaagata tagatattaa taatgttaaa aatggcattt atactggaag
     9241 tgctgataat aacttggtaa aagctactgt gtctgttgaa gttaataatg gtaaaatcca
     9301 aaatattaat atcttaaaac acgaccatct tttaggtaaa cctgctgaaa aaattacaac
     9361 aagtattatt aaacaacagt ctttagatgt ggatgctata acttctgcaa cttatagcag
     9421 taatactata cgaaaagctg tagaaaatgc tttaagaaag ggagaataat tatgaatact
     9481 attattatat attcaagtaa atatggttgt acaaaagact gtgcaaatat actaaaaaat
     9541 aaactatcag ataatgtcac tattgttgat attaataata ataataataa aattgagtta
     9601 tcaaaatttg ataaaattat tattggaagt tctatatatg ttggttcagt ttcaaaaaaa
     9661 atacaggtgc tatgtaatga taatgtagag ttattaaaca aaaaacaagt tggcattttc
     9721 ctttgctgtg gcttttctga acaagcagat aaatatttaa aatcaaattt tccatcatcc
     9781 ttattagaaa gtgcaaatgc tattggaatt tttggtagtg aagctcgatt agaaaaaatg
     9841 aaatttttag ataaacttat tatgaaggct gtttcaaaag gtaattatga cagttttaga
     9901 atatcacagg ataatataga taatttttta ataaatctta atagctaaaa taaagctaat
     9961 tatttctatg ataaattctt ttatttataa gtaatattgc tcaaaatagt ctttaaactt
    10021 tatcaaaatt ttatttttct tagtgaaatc taatgaaact agcacttaat atatgcttta
    10081 ttaatcataa caaatatgtt ttattaatca taacaaaaag agagtgtcta atgaactaaa
    10141 aaattcatta gacactctct ttttgtatga aatcaaatat atttttatca tttcaacaga
    10201 gaaaaataca cttgtctcta tgtttggcac attctctttt tataatataa tgtttaattt
    10261 aaaactacta tttcaaatat tgacatatac ttaaactata gacttacaaa atcaattcat
    10321 attagcttaa aatataatct ttgttattca aattcctata cacaaccatt atttactttg
    10381 atactttaaa acatcttccc tattttttat taaattttat aataatagtc ctattaacgc
    10441 aaatactata aaaagagttt aactttatcc attaaataaa tttaaattaa aacatataga
    10501 aatggaagga gaacaattta tgataaaact aatcggtata ttgatagttg taattggatt
    10561 tatacttaaa attgacacac tatttactgt attattagca ggagtggcaa caggaattgt
    10621 tgcaggatta gattttaatc aaatttttac aattttagga gattcttttg tctcaaacag
    10681 aggtgtatca ttattcatat taacattgcc agtaatcggt gtattagaaa gatatggttt
    10741 aagacaaaga gctgtttctt taatagaaaa attaaaaaga cttacaacag gaaaagtact
    10801 tactatatac atgatagcta gacaaatagc tggtgctcta tcaataagaa tgagtggaca
    10861 tcctcaattt gtaagaccac ttgttaatcc tatggcacaa gctgctggtt taagtaatag
    10921 tgatgaacta aaagaatcgg atgaagaagc cataaaggca ttatctgctg catcagaaaa
    10981 ctatggaaat ttttatggtc aaaacttatt tgcaggtagt agtggagtat tacttatagc
    11041 atctactctg actcaatttg gatacaatgt aactggactt aatatcgtaa aggctagtac
    11101 tattatggca gtaatagcac ttgtagtggc tacaatccag tttaccttat atgataaaaa
    11161 attaaacaaa agtcataaaa aatagtttta tttaggagga ataaaaatgc aaaattttat
    11221 aaacatatca ttggaaatct tttacgcact aatgggattt ttaatgattg ttatagcata
    11281 taagtctttt actaccataa ataacaataa gaaatatggt acatcattat tttggatact
    11341 tatatcactg ccattcatat ttggtagatt gattccagcc aatataatag gaataatatt
    11401 aatattatct agtttattaa cactttctaa acaagttgta tttgcaaagt atgaagaacc
    11461 agatgaaaat tttggaaaag aacaagcaga caaacttaaa aataaaatat ttataccatc
    11521 attaatatta gcatttgctg cagtagtagt agctatgagt ctttctaatt ttgataattc
    11581 ttctcagttt gcaataggtg tgggttcaat aattgcatta atatcagccc ttataataac
    11641 taaggcaaaa cctgctacat cagttcaaga tggctcaaga ttacttcaac aaatgggacc
    11701 tgctagtatg ttaccacaac ttttagttgc attgggagct ttatttactc aagccggagt
    11761 cggtgaagtt atttcaacca tgatatctgg tgtagtccct gctgatagca gattatttgg
    11821 tgttatagct tatgtattag gtatggttat atttaccatg ataatgggaa atgcatttgc
    11881 cgcattttca gtcataacgg caggaattgg tattcctttt gtattgtctc aaggtggaaa
    11941 tcctgctata attggagcat tagcattaac agcaggatat tgtggaactc tacttactcc
    12001 aatggctgct aattttaata tagttccagc agccttgcta gaatgtaaaa atgattatat
    12061 tgttataaaa tatcaagctc cagttgcttt ggtattaatt atagcccaca tattagtaat
    12121 gtacttttta ggattttaat ataattagga ggtaaaataa tgaaaatttt gttaacagga
    12181 tttgacccat ttggcggaga accaataaac ccagctcaag aagcagttga aagggtcaat
    12241 aataatataa acggtgcaga aatcataaag ataactatac caacagttat gacaaaatct
    12301 gttgaagcta tagataaagc tattcaagag cacaatccag atatagtaat atcagttgga
    12361 caagctggag gtagatttga cattacacct gagagagttg ctataaatat agatgatttt
    12421 agaataaaag ataatgaggg taaccaggtt atagatacta ttataaaaga agatggtgaa
    12481 cctgcatatt tttcaaaatt gcctgtaaaa gctatggtaa aacatatgaa tgaaaataaa
    12541 attccagcta gtgtttcaaa cactgcagga acatttgttt gcaatcatgt gatgtatgga
    12601 attctttata tgatagacaa gaaatatcct aatataaggg gtggatttat acacatccca
    12661 tatacaactt cacaagtaat agataaaaaa aatacaccat tcatgtcttt agaagaaata
    12721 gtaaaaggct tagaattagc tatagaagct tgtattatat ataaagaaga tgtaaaagaa
    12781 attggtggtg aaatttcata ataaattttt tagtattttt ataaattaaa aatactaact
    12841 attaatttaa atatagactg tgagtagatt attcaaacaa ttttactcac agtcttttta
    12901 tggtatctac agatacaaaa ttaaggtcta ttcctctctc cccactcaca cattgcatct
    12961 aaaattggaa tcagagattt tcccctttct gatagactat attctacctt aggaggtatt
    13021 tgaggatatt cagtacgaat aatcaagtta tctgcttcta attcttttaa tgataaactc
    13081 aaagttttat aggagatagt atttatataa cgttttaatg cattatatct tacaacttca
    13141 aattctacaa gacagtaaag aattatcatc ttatatttac cactaatcag tgaaagtgta
    13201 taactaaatc ctgtttcact taaatctgta tttgatatac agtctatact catatattac
    13261 actctccttt tggttagtat tgaacaaaaa tgtgcgtact tgtttttaga ataatttata
    13321 ataaaattat agtgtaagaa taattgaaag tcaattttat aataggaggt gtatttacta
    13381 aaaaaaaagt tattttaaat aggattctaa gattagacat ttctatataa attgataata
    13441 ttcatgaaaa tcctatttta taaaaaataa tgtctcttag aaaatctatt aaataaaatt
    13501 cattggagga aatttataat gaaagtttta ttgataaacg gtagcccaaa ccaatatgga
    13561 tgtacttata cagcactaaa tgagattact aaagtattga gtaaacacga tattaaaaca
    13621 gaaatactgt atttaggtaa agaaacaatc ccaggatgta ttagttgcgc atcctgtttt
    13681 gaaactggaa aatgtattcg aaatgataaa gttaatgaat tgattgaaga tctggataat
    13741 attgatggta ttattattgg ttctcctgtt tacttttcta gtgcaactgg tcaattaact
    13801 tcttttcttg accgtctatt tttcatagct gggagtcgca tggctacaaa attaggtgct
    13861 tcagttgtgt catgtagacg tggtggtgca agtgcaacat ttgaccaact aaataaatat
    13921 ttttcaatca gcaatatgcc tattgtatca tcacagtatt ggaaccaggt acatggcttt
    13981 actccagagg atgttatgaa ggatgaagaa ggattacaga ctatgcgaac tctaggagaa
    14041 aacatggcat ggcttcttaa gtgtatatca gcaggtaaaa aagctggaat taaagaacca
    14101 caatatgaag aaacaattat gactaatttt ataaaataaa taaaaaatgt actaatatta
    14161 attaaaacaa aacactaatt tataaataaa ataagcataa atgtaggata aaaagcacta
    14221 aaacagaggt attgaatttt atatcaaacc tctgttttat tattacatta ataaccaaac
    14281 catgtataac atcatatatt tctaaataaa ttataatatt atattctaat gttattgaaa
    14341 ttacaataac tgaatttctg ctcataaatt taaatattct acaaatctca tctaaataac
    14401 aattttaata gacctaaaac aatcctcata ctgttcattt tgtattaaaa atatcactta
    14461 caatcaaaaa ccatttatta agtatattcc taagatatac aatatatata catggattgg
    14521 atagtataaa taaaataaat acttcatact cttccctttt tctccattgt aaagtaacat
    14581 taaaggtaga gaagcaatca tcatccattg gtaattttgt aaaatagaat tttttaaact
    14641 ataatctcca tttgacattg ttgtaaatat aaatatacac attaaaacat aacaaatact
    14701 caaactgaac tttttacctc tacacaaata aaaaataatc ccaagtataa tccaaattgg
    14761 tccaccttct actaaaaatg gtactggtac caaaatcata aataaagcaa taactctgtt
    14821 agtgacttta aaaagtaaag ctactgtaaa tattcctaaa attataggaa tgattatcat
    14881 caataatcct ataataaaat atttaatttc tttctctttt ctaaatttct ttatgaactc
    14941 tattgcttgt aaataaatcg ctattaaaaa taatgttgaa aaagtgttat ttataattat
    15001 agctccttct ggatgtgaaa atacattatt aattatttga tttcctatag ccataataac
    15061 agaccaaata tacaatctac ttatatattt ttttctgtta ctagtgtgat aaaatccttc
    15121 aacagccatg aatataaaca gtggtgctga tattctgcct acccaatgaa accaaattgg
    15181 tatttgcatt gtttgtggca taaatgctgc tatatggtca aatgtcataa atattaatgc
    15241 taaaatctta atagtaaacc cacttatacc ttttttaaaa aaatccatat catatctcct
    15301 aattttgttt tatctctata ataatttttg ttcctattcc aatttcacta cttacataaa
    15361 ttttaccttt atgaatctct attatttctt tagaaatagc cataccaaga cctgaacctt
    15421 tatgcatctc acctgtatta gtacctctat aatatttttt aaaaatatgt ttcaaatcac
    15481 ttttgcttat tccaattcca tcatctttta ttataatttc tatattatct ttttttacaa
    15541 tttctacact tatcaaagtt ccttcgctat tatgaactat tgaattaaat ataagattgg
    15601 ttatagccct tctgaataag attgaatcta catatacttc aattaaatct tcattacttt
    15661 caaattcaat attcctattt ttatatctat tatcatttaa aatatcaatt attatatctc
    15721 tgactaaact gactaaattt atttttttct tgtccagaac tatagtatta ttttttaatc
    15781 gagtagataa atttaaatca tcaactaaat cttttatata cttagactta ttatatataa
    15841 tttctgtata ctcttgtatt tcatcttctt caaactcata atctttgtca ttaattatct
    15901 ctgcataccc ttgtatagat gctagaggtg tctttatatc atgtgttata tttgccaacc
    15961 attcctctct caactcttca ttttcttttc tctcaacttc atttactctt aaagtatcag
    16021 ctaacatatt tatattttta aaaacttctt catatatacc tctgtcctta agatataaac
    16081 tataatttcc attagccata gtctcaatac tccatagtac gcttgaaatt ggttttgtaa
    16141 gttttctact aaataaatat cctacaccta aagctaccac gctatcaatt actagtgtta
    16201 taatgataac tttatttaaa gtttttttaa tgttattttg agaataggtt aaaattattc
    16261 ttgatatatc tctactaggt atacctacta ttatgtttag atgttttttg tttaaatatt
    16321 tttctaatac aaaattaaca tactttgttt ctatatactt gtaattattc accatttgaa
    16381 agggtgtata ttttttggga gtatcctttg gcacatttac tccataaact tcctctccta
    16441 aatcatttaa aacctgtatc caggaatttg atttttctaa tttttcagct ccaatcttag
    16501 atagtttaaa ttctccatct ttttcatata aatctttttc aaaagaacgt gcaaattctt
    16561 caggattatt tccagaatct acaactttaa aaaaactatt agttgaaatc acatacaata
    16621 tagatatgat atttataatt actactgata tagctacaaa cacaattgta aatataaaat
    16681 ttctagtaat cttccacttc atattctaat cttcctttac tgctaattta tatccaagac
    16741 cttttattgt cagaatatat tcaggctttg aaggattatc ctctaatttt tccctaattt
    16801 ttcttatatg aaccattata gtattgtcaa atccaacata atcttctccc cacactttat
    16861 cccatagttt ttctttactt ataatttgat tttgattatg tgcaaataaa gataggattt
    16921 ttagttcctt tgcagacagt cctatagatt ttccatcttt tataagttct gccctacttt
    16981 catttaatat atatgggcca aatgatataa ttttttcttc attattattt agatttttac
    17041 tttcgttttg agcatcacta aaataactta atcttctcaa atgcgcttta actctaaaag
    17101 ccacttcctt agggctaaaa ggttttgtaa cataatcatc tcctccaatc gctagtccaa
    17161 gtaacttatc taattcttcg gttttagctg ataaaaatat tattggagaa ttggaggttt
    17221 ttcttatttc tttacaaata tcataacctt cgccatctgg taacataata tccaatacaa
    17281 tcaaatcagg attaatactt ttaaataatt ctaatccttc ttttttagtt tttgcagtat
    17341 atatattatt aaggccctct ttttttagga cagtttctaa aagcttcaat atttctactt
    17401 catcatctat tattaaaaca cgattttcca taaacaacct cctgtaataa tttacatata
    17461 tattagcata tattttatct aaaattcatt tatcttattt ttaattaaac taaaatataa
    17521 ataaaattac tattattttt agttaaaacc ttttgcttga tgtaaaattt tcatatgtta
    17581 ttttagttaa cactatcatt aatataattg agattactat agaaaaaact atcaaaatac
    17641 ttggtacatt atacttaacc atttgttggc tcatcataat ccaatttgga gtcactgaca
    17701 taaattttat agctttatct ggtataaaac ctacactaaa gtatggaatt gccattatca
    17761 atccacctag tataaatgga attacagccg atttaaatat agatgaaagt gttaagccta
    17821 tttgtgcaaa tataaaagaa gcaaaaatag tagtcaatat aacaattata tacatttgta
    17881 ttactgtaag atttaaatta ctatttccaa agtaccataa gttatgaatc gattcattta
    17941 aatttttaaa atcaaaaaaa ctaaaacctc taattcctac taacaatata aaagaaatat
    18001 taataataga taaaattaat ccattagcta ctagttttga taatactact gatttttttc
    18061 cctttttagt tgtatttatt aaacctgtca tattagaatc atattcttta atgtaatttc
    18121 cagaagttat tataaacatt atcaaaccta ttagaggtat tgagccttct atcttgtaaa
    18181 atatatctgc ataagtatct aaaacatcaa actttctaat cagaaaaaat ccaaaaaata
    18241 ttaaaaacag ccaaattatc caaacactct ttgattttaa tatttttatc atttcagctt
    18301 taactaaata catcatttct actacacctc ccttgcatct tctaaatcaa aataaaacat
    18361 gtatacatct tcgaattttg cttgaactgg ttgtatatta tcctcgcttg gaatatcctc
    18421 tgaaacaatt ctaagtacta ctgaatcaat atcactttga atacttacaa ccttatattt
    18481 agattgtata tcatcaactt cacattcatt tttaacttga acattatata ctttattttc
    18541 catgtgactt aatatgtctt tgtgtgttcc tttcattagg agtttgccat ctttaatcat
    18601 tattgtttcc tttgcaactg attctatatc ggaaattata tgagtagaaa gtattattat
    18661 cttatctcta gacatttttg ctattaaatt cctaaatcta gctctttctt gtgggtcaag
    18721 tcctgctgtt ggctcatcaa ggactattat cttagggtcg tttagtaaaa cttgtgctat
    18781 cccaactctc ctcttcatac ctcctgaaaa ttttcctata gacttatttc tcacatcata
    18841 aagacctact aaatctaata attctcctat cttactattt aaatattttc cccttaaacc
    18901 ttttaaagct ccaatatact gtaaaaattc ttttgctgta aaatttttat aaactccaaa
    18961 ttcttgtggc aaatatccta aaatatttct ataatcagca tccattttaa atatatcctc
    19021 accattataa agtatctgac cttcatcagg ttttatcaac gttgttattt gtttcattaa
    19081 tgtagatttt cctgctccat ttggtcctag aagaccataa actccatttt ccagagttac
    19141 tgtaatcttg tcgtttgcta cttttttacc atatatcttt gaaagatttt ctatttctaa
    19201 tttattcatc ttaactcccc ttttatctac ttatataatc tgattttatt actaaaaaac
    19261 ataatgacat agcacctatc aatattgcca tcaaaagtcc tattgctata ttgacaatgt
    19321 ctatattcac tttaaatatc ataatatcac ctagataatt tcctattatt ttattaaaac
    19381 caaatatcaa atcataataa tttacatttg atacaactga aagtaatttt ttaggtaata
    19441 tatggattac cgacattatc ttagttatag atataataaa tataaaaaaa cttattgaag
    19501 atattgaaga cttacttata aaagacccaa atatggctat catggcccat ccttcaaaca
    19561 tcaaaagctc tatgcctata tttaatatta catactttgt tatacttaaa ttagttttca
    19621 atgcaactac attgtctact attctaaaag attctaagtc tccattaaca ggtactcctt
    19681 gttgcataca agttataata aatattccca ttaaataaac agaataaatt actaatggaa
    19741 ctaataaagc aacaaatatt ttagacttta atgctttttt cttctctttt gatgatagta
    19801 tgatatcttt taccgaagta acgacttcat ctacataaat attggatata attatagata
    19861 taatgattaa cattcctaca tttataagtg aatttgtgac tctatagtta aatactttcc
    19921 aaaaactaat atccttatat tcattcacac ttagattatc aagttttttc tttgacatta
    19981 attcaatttt tttagaaaat tcacctgtat ctgtttgatt tgaaaactgc tttaaaacat
    20041 taatcttatt ttgaaatttc tcattaccta cgtcgatacc acttctcgta tcttctatac
    20101 gtcctttttc tttatctata taataacttt ctgtttctaa aacaggcgta ataaagatac
    20161 taatcattag tattaacact aatatcccta gagatataaa catactctta gatttagcca
    20221 atttttttat ttcccaattt accaatatat ttctcctcct aaatcatttt aattacacat
    20281 atatattaaa actta
//
LOCUS       DAMJYR010000060        20197 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.060, whole genome shotgun sequence.
ACCESSION   DAMJYR010000060 DAMJYR010000000
VERSION     DAMJYR010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 20197)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20197)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20197
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.060"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            424..786
                     /locus_tag="PC471_002200"
     CDS             424..786
                     /locus_tag="PC471_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HDF2789328.1"
                     /translation="MENKILIVDDAIFMRMIIKEALSKSGYNNLIEANDGEEACNIFK
                     YEKPDLMLLDITMPKKDGLEVLREIKKLDSSAKVVMCSAIGQENMIVEAIKLGALDFI
                     VKPFKTETLVKVVDNALK"
     gene            851..1468
                     /locus_tag="PC471_002201"
     CDS             851..1468
                     /locus_tag="PC471_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheC"
                     /protein_id="HDF2789329.1"
                     /translation="MINYLELDDVHIDALREIGNIGSGNAITALASMINSNVEVLIPM
                     VKILEYNEATNLLGGPENKVVCILLDMKGDINGMFMFLLDESITQLMLSSLFNKEEAF
                     LDEIEAIEISAIKEIGNIMASSYVNAIASMLNMTISVSIPDICIDMVGAVLNVPMIRF
                     SDVGDKVLFIENKFKMSDNYFTSHILMIPEMSSLREILVRLGLEV"
     gene            1465..1950
                     /locus_tag="PC471_002202"
     CDS             1465..1950
                     /locus_tag="PC471_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheD"
                     /protein_id="HDF2789330.1"
                     /translation="MNREIVVNIADMKIAYRPNVLVTYALGSCVGVCLIDKVAGIGGM
                     LHVMLPYSKDAINIENKCKFADTGINELIKSMENIGANRIYIKAKIAGGAQMFLGSSN
                     SVIASIGHRNVEAVKKTLLGLNIPILAEDTGMNYGRTIRFYTETGELLVDSINKGKKK
                     L"
     gene            1993..2511
                     /locus_tag="PC471_002203"
     CDS             1993..2511
                     /locus_tag="PC471_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-binding chemotaxis protein CheW"
                     /protein_id="HDF2789331.1"
                     /translation="MRFPDDPYLDYGLIDEGIKYLKFKVDEQDFGVELEKVIEITGNQ
                     EIIFIPELPTYSKGIINLRGKIIPIIDMRLRLKKDELMTTNCMYIVVTEIKDLVVGFV
                     VDRVDDIVSLEKSKISPPPRVTIEYSDYFVSGVGELEGKIITLLDLEKLLTDKDEVLI
                     DELINGFDCTIM"
     gene            2647..3606
                     /locus_tag="PC471_002204"
     CDS             2647..3606
                     /locus_tag="PC471_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HDF2789332.1"
                     /translation="MKNKQQGTILVVDDSVLMCDLIDKTLTKDNFKVKKAFNAIEAKE
                     FIEEFIPDIILLDIILPDNSGFEFCKEVKSNARTADIPIIFITSKNTDTDIVKGFGVG
                     AIDYMVKPFSMTELKARVIAHLEVKKSQDKLKKINNELESSLEKLNRLVVRDYLTGLY
                     NRRHIINQLMEQRRINKYSETSISLILGDIDDFKVINDTYGHEAGDFVLTVISSIIKK
                     NCRQIDTVSRWGGEEFLIMLTNTPIEGAKILSERIRKEIKEFDFNYKEHKIKCTITLG
                     VVEVNSNISLEENISLADKAMYEGKNLGKDCSVVSTMKGLKKI"
     gene            3632..5335
                     /locus_tag="PC471_002205"
     CDS             3632..5335
                     /locus_tag="PC471_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HDF2789333.1"
                     /translation="MKREVKLSKLAIVLLIIIVLAVLAGIFSSISSYKAFEKVEKSIN
                     DMNNLRTQSQYIKDTTREISDVLRRYVFTGEKKNKDIYEKELYGKTRESALNKLEKIG
                     LTKSESNKILEAKEISDSLVPTEQSAIKAVESNDKSKAQSIIFGSDYINSQEKMDSLI
                     NEFQEDIVTRSNSKIVEVKKELQSLILRTIFILSLLIILTIIEYTVIKLKIVIPINKV
                     EKHFSRIASGDLRTSIDVEESKSEIGLLVSSVKKMQSMLLNYIDLIDSTLTSIAKGDL
                     RVEIDQEFIGDFKSIKISLESIINSFNGDFSEINLSAEQVASASEQVAAGAQALSQGA
                     TEQASSVEELASTINEISDGIKKCADSASLACEVSDQSASQVGFGSRCMDDMMTSMGE
                     ISEKSSEISKIIKTIDEIAFQTNILALNAAVEAARAGQYGRGFAVVADEVRSLAGKSA
                     KAAQNTAILIEEAIKTVENGVRIAKETANALDLVVNGVEKTTNYINGISSAVHSQEVA
                     ITQILSGIEQISIVVQMNSATAEESAAASEEMSAQAQILKELVEKFHLKESEVRESSW
                     V"
     gene            5596..7698
                     /locus_tag="PC471_002206"
     CDS             5596..7698
                     /locus_tag="PC471_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HDF2789334.1"
                     /translation="MRQMLDMFIFETSLLLEQLDEIMIQIEKEKVFTHNNINETFRIM
                     HTIKGSASMMGLDNISALAHSVEDLFYAIREGKIEVLDNDLLFDLVFQSLDFIRRDID
                     ELQSRGCISSDYSNFIEGIEEYVSTLELSSSMKSKDNANLESKDEKEVIDSNFEYKED
                     SDVVKIIFEQGCMMENVRAFMLVFELRDYCEFIDFYPKDVETNAESTEYIKSYGFYIQ
                     FVAKEDKAIVYKTIEDNLYVKEYINISKKEIEMKSTSNNDAVENKLKDNNKVSKSYQN
                     NYTEDEIQEDVLNEKTDMEKDSLSEENEHLPISKQSIMSVNLEKLDLLQNIVGEIVIM
                     ESMVVNSVNSTGVDIDNFNKATRQLHKLTDELQDIVMSMRMIQIAGVFQKMHRIVRDM
                     NKKLDKNVELITIGAETEVDKSVIDNLAEPFMHLIRNAMDHAIEPIEERIAKGKEEVG
                     KIVLSAENLGGEAVISISDDGRGIMRDKILAKAKEQGLLKHQSNEYTDDEVNSLIMTP
                     GFSTKESITEFSGRGVGMDIVRKSIEKVGGSIEVKSKQDEGTTFTIKIPLTLSIVEGM
                     KFKVGDSLFTLPISSIRRTFKLTDLNQIITEGNKTELITIYGACIPIIRLHKIYNIQT
                     KVTNLMNGNMIIIENEHKAVCIFVDEIIGNQQVVVKPFPVYFNRFNIKNKGLSGCTIL
                     GDGSISLIIDADSIIEIY"
     gene            7711..8151
                     /locus_tag="PC471_002207"
     CDS             7711..8151
                     /locus_tag="PC471_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HDF2789335.1"
                     /translation="MAKDEIIKQVLTFYVNDVIYGIELENVIETIRFQSITYVPCLPT
                     YISGVINLRGRIIPVINMHIKYNLPKADYNERTCIIITKVDEYQVGIIVDKVVDVIHI
                     ENLNLLETTNSNNTNINKDIKEIAKVEDNSILILDIRKFLINSI"
     gene            8187..8990
                     /locus_tag="PC471_002208"
     CDS             8187..8990
                     /locus_tag="PC471_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HDF2789336.1"
                     /translation="MIKLTDEEFIVLVNHVKKEYGIDLSKKRALIEGRLYNTMIEKKL
                     SSFSQYMNLLFKDRTGNEAINLINRLSTNHTFFMREPQHFEFIQNSILPFWEENNKIR
                     NINIWSAGCSSGEEAYSIAMTLDDYFGYNKELWNIKIIATDISTNSLEKAKRGIYIES
                     NVNNVPELWKKKYFIDNKDGTFKICDKIRKSVIFKPFNLMNDFSYQHFDLIFCRNVMI
                     YFDLKTREELINKFYNATKKDSFLFVGHAEVINRSSTKYKYVKPAIYKR"
     gene            9030..9626
                     /locus_tag="PC471_002209"
     CDS             9030..9626
                     /locus_tag="PC471_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheB"
                     /protein_id="HDF2789337.1"
                     /translation="MERFNNMVIAIGASVGGTEAILEIIKDLPKSTPGIVVVQHMPAI
                     FTYMYAQRLDKQCIMNVREAKNNDRVEQGNVLIAPGGYQMKLCTDKQGYYVTCEKGER
                     ISGHCPSVDVLFDSVAEVAGKNSIGIILTGMGYDGASGLLKMKENGSFTIGQDENSCI
                     VYGMPMVAFDRGSVMLQLPLGEISNCLIRHINILKQKD"
     gene            9732..10595
                     /locus_tag="PC471_002210"
     CDS             9732..10595
                     /locus_tag="PC471_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 and DUF4163 domain-containing protein"
                     /protein_id="HDF2789338.1"
                     /translation="MKFFKVDTKAKVLRYVFVLTLTIMAVLAIKHDYKYNNLRKDASI
                     NLNSNKEAVTVMSNQSLPKGNTNSKQTLSVSTIGSKTIVKNDEYLESTINMPVITNSN
                     KIVERSTNDKIKNDIFEFYNKSYKEAKQYLKDNPDEKNKFVANVDFELKKNTDSALSI
                     KVRYYTYSGGAHGFYQDIAYNVDMRTGKFLELMDLFKDNTKYKEVIDEEIKRQIAELE
                     KKDEENIGIYNFKGIKENQNFYLQDENLVVYFDLYDITPYAAGIPEFSINKALISSML
                     KPEYVDLFDLK"
     gene            10662..11651
                     /locus_tag="PC471_002211"
     CDS             10662..11651
                     /locus_tag="PC471_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HDF2789339.1"
                     /translation="MLLLKKDDIKKVFTMRDAIEADKEAFRIYCEGKSVNPLRTNISV
                     PSQDASMLFMPGYVEELGCGGIKIVSVFPKNAQKGKPVIPATVLLLDGETGEVSAVLD
                     GTYVTQIRTGAASGAAIDVLAKKDSKIGALIGLGGQGEPQLEAMIEARNLDEVRVFSR
                     NKESREKFAEEMNLKLSKYNTKIVAVESSDEAIDNADVIVLATPSKQPVLNGNLVKKG
                     ALISAVGSYMPDMQELCPNCLTRASKIFFESTDAVLSESGDILIPLKEGKISKDDFSG
                     DLGNVINGTIPGREDDDEIIVFKTVGIGVQDVVTAKRIYDKAKENGIGIDWKW"
     gene            11743..12354
                     /locus_tag="PC471_002212"
     CDS             11743..12354
                     /locus_tag="PC471_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HDF2789340.1"
                     /translation="MKMVKKLILSTIMALVLVLTIGCSNAEEKVKSNFEANLAALEMY
                     KYDEKGISDALTEAEYKQIQTYNSKDKVSMIAQLTPLYGKENAEKIYETIVKLIQGVE
                     KTVTVESSSKDKITLKVVSKALDTSKVMSEANTLVKSYVTDEKNYDKKEKEVQDDMTK
                     MIIDLYNKKPTKEVTTMITYTKKDGKWQPPSNIQKLCEGIYTI"
     gene            complement(12501..13328)
                     /locus_tag="PC471_002213"
     CDS             complement(12501..13328)
                     /locus_tag="PC471_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HDF2789341.1"
                     /translation="MKIKDNKAVFHVFIRIFLIAVLILTGTLLEEKINNVPVENISSK
                     GENTLELLSQSNTLGEIIYKASQEETKGPGATSEKVAYITIDDGPSKYTNALLDILKE
                     NDVKATFFMLNQNMKNHKEEVRRVLEEENSIGFHSVSHDIHKLYKSPEVTVGEFNTCN
                     NTLQEITGQSSKLIRLPYGSKPYMPEGSYNKLMANDYLIWDWNLDTQDWKSSTSQIVS
                     NILYYGRKRDELVVLIHEKEQSLNALNNIIRILKERGYTILPITENITPKNFWQENL"
     gene            complement(13376..14812)
                     /locus_tag="PC471_002214"
     CDS             complement(13376..14812)
                     /locus_tag="PC471_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron hydrogenase"
                     /protein_id="HDF2789342.1"
                     /translation="MNTRNDNSKILEIYKMKLFGELVKAVWNDDIEDIENLPNKILNE
                     NLKDNLNNYFSGKFDKDTMVNLIRVTMGLDVTEDTDKNLKESLHEAVNLDEVKRPIIS
                     VISDACKYCDENNKEDESCKIRSKHINCNEEDVCSACGDCVSRCKLGAISDKIEFIPM
                     IKLLKDIKSPVYAIVAPAFVGQFGKDVSPGKLRTALKKIGFEDMIEVALAADMLTAKE
                     AYDYYEHMKKDEEGCFITSCCCPIWVSLIQNSFPQILENVSPSVSPMIACGRAVKILN
                     PDAKVVFIGPCMAKKKEAMLEDIKDAVDFVLTFKELQEIFDALELNPAIMDESNRVEA
                     SLSGRVYARTGGVSKAVKLSVRRIDKNIKFEEKAFEGTKECMDGLKKVLNKEIEATFI
                     EGMGCVGGCVGGPKRILSVEEGTKNVDEYSESTNMETPFDNLNVLQFLTSLGIKRVES
                     LGEKEEEKVKKIFSRDIKSNKKENKYKK"
     gene            complement(14899..15768)
                     /locus_tag="PC471_002215"
     CDS             complement(14899..15768)
                     /locus_tag="PC471_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HDF2789343.1"
                     /translation="MKLQKMGMRTIKTGISVTLCMILGDYLVENMFYSLVACIISVQD
                     TVKGSLKTGINRVEGTILGGIIGFIFAIIKAGDPFLCGLGVMCSIYLCNLFKIKSVVV
                     ACVTFLAIHLGVGNSDPVYYSLHRVLDTSVGVLFGVLVNYYVARPNYLNSVIDEFKKI
                     EELSMLLIEDKILRKENLNVEKFEEEVEKLEEIYSKLIDELDYSLSETNIEKIENALR
                     ICREIHFHMQSIELLENKLYLKEHTYKYLKKTYNVDKLDWNIDEEKSPVFNYHLMKIM
                     IEIELLRKLNKSY"
     gene            complement(15926..16579)
                     /gene="phoU"
                     /locus_tag="PC471_002216"
     CDS             complement(15926..16579)
                     /gene="phoU"
                     /locus_tag="PC471_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HDF2789344.1"
                     /translation="MVNNSLDISIDTLKEQTVLMMEKCEEAIEKSVTCMIEKDLEGAR
                     KVVEDDEEINKLREEIRDKSIELIVLKQPMARDLRFIYSLGNISIELERIGDYAANIA
                     REMLKIGGEAYIKELIDIPKMAKECKSMMSQTREALILFDASIAYDVGTKDDIVDGLY
                     EQVQIDCLKIMHENSNTIDQGVKLLLIGRYLERIGDHITNVCEKIIFAIEGKMVEIG"
     gene            complement(16607..17371)
                     /gene="pstB"
                     /locus_tag="PC471_002217"
     CDS             complement(16607..17371)
                     /gene="pstB"
                     /locus_tag="PC471_002217"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HDF2789345.1"
                     /translation="MELIDKIKMSVKDLDLFYGDKQALKKINMDIKENKVTALIGPSG
                     CGKSTFIRTLNRMNDLIEDVTIKGNISVDGEDIYTSDDVINLRTKVGMVFQKPNPFPM
                     SIYDNVAYGPRTHGLRDKKQLDKIVEESLKGAAIWDEVKDRLKSSALGLSGGQQQRIC
                     IARAIAMRPEVILMDEPTSALDPISTLKVEELIEDLKKDYTIVIVTHNMQQAARISDE
                     TAFFLNGEVIEFSDTKTMFTTPVDKRTEDYITGRFG"
     gene            complement(17390..18214)
                     /gene="pstA"
                     /locus_tag="PC471_002218"
     CDS             complement(17390..18214)
                     /gene="pstA"
                     /locus_tag="PC471_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003460575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HDF2789346.1"
                     /translation="MRKFKENLLKSLVYLSALFTIVSLVVIVGFIFIKGIGNMNFNFL
                     FSNYSASGDGGILPMIITTLYTVVVSIVVATPIGILAAIYLQEYAKKGKAVTAIRFAT
                     ESLSGIPSIVYGLFGGIFFVVTLKMGYSIVAGSLTVAIIILPVIIRTTEEALKTVPQA
                     YRESSLALGATKFQTLYKVVLPSAIPGILSGVILSVGRIIGESAAILLTAGTVAKMPG
                     GIFDSARTLTVHSYLLTKESGDIATAASIGIVLIVIVLALNMLARFVAKKLNKANY"
     gene            complement(18216..19133)
                     /gene="pstC"
                     /locus_tag="PC471_002219"
     CDS             complement(18216..19133)
                     /gene="pstC"
                     /locus_tag="PC471_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HDF2789347.1"
                     /translation="MIVKSRLVGLEKNDNNGNKTKYVIEKVAKNIFLLSALVAVASLL
                     LIIGFVFYKGLRPFIFEGYSFIDFLTGADWVPSANKFGISSMIVASIFATLGALVIGV
                     PVGILTSVFIAEVAPKRLAKIMSPAVELLAGIPSVLYGVFGLAVIVPTIQSIFNLPKG
                     QSLLAIVIVLAVMMLPTIISVSETAIRAVPKAYKEGSLALGASKIETIFKVVLPAAKS
                     GVLAAVVLGVGRALGETMAVILVAGNSPVMPTAITDSVRPLTTNIALEMGYAFGTHQE
                     MLFATGVVLFTFILILNLVLNKLSNKAVN"
     gene            complement(19514..19960)
                     /locus_tag="PC471_002220"
     CDS             complement(19514..19960)
                     /locus_tag="PC471_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2789348.1"
                     /translation="MAISDLAFGTECLKTSFQMLFSVISLVNINLLQAQILIAIGGLI
                     SILAMVSLTLFLSAKCKDSLTSMLIAFSICLIPMGIGVSWIGYLLPPGGIGFKISLLY
                     QMTSFNFLHLGGMSFWTPHIILFFSVIEIPIFIFLAIHTYCKHQVS"
BASE COUNT         7503 a         2662 c         3165 g         6867 t
ORIGIN      
        1 aatcgataat acatatagag aaatataatt aagtaaataa acaactatct attagatatt
       61 gagaaagtca aatcctatat gaatctacag aaatgatttt aatgaatctg aaaatttttt
      121 ttatacaaac ataaaaaagg tataaagttg ttttattata atttaattac ttttgataac
      181 tttttatgtt ttatacaatt atatttttaa taactcgtca aaacgccaaa catatattac
      241 aatagatata taagtttttt tgttgacaaa ttatgttaag agaattaagt ataatatggc
      301 ataaataatt acttttattt atcaatgaaa tgtatttatt gtgtagcgaa aaatagtcag
      361 atagtataga caataactga agataaaagc gtttattgtt gtacatatgg aggagttagt
      421 tttatggaaa ataaaatttt gatagtagat gatgctattt ttatgagaat gataattaaa
      481 gaagctctga gtaaaagtgg ctataataat ttaatagaag caaatgatgg agaagaagcg
      541 tgtaatatat ttaaatatga aaagcctgat ttgatgttgc ttgatattac aatgccaaaa
      601 aaagatggtt tagaggtgct aagagaaata aaaaagttgg attcaagtgc taaggttgta
      661 atgtgctctg caataggaca agagaatatg attgtagaag ccataaaact tggagcgtta
      721 gattttatag tgaagccctt caaaacagaa acattagtta aagttgtaga taatgcttta
      781 aaataaaagt gtaaatatac actaatacaa tgaaaagcaa ataaatgcaa taataaaggg
      841 aggggtttat attataaatt atttagaact tgatgatgtt catatagatg ctcttagaga
      901 aattggtaat ataggctctg ggaatgcaat tactgcactt gcatccatga taaatagcaa
      961 tgtagaagta ttaataccta tggtcaagat acttgaatat aatgaagcga caaatttgct
     1021 tggtggtcca gaaaacaaag tagtatgtat tttactcgat atgaaaggtg atatcaatgg
     1081 aatgtttatg tttttgttgg atgaatcaat aactcaactt atgctaagtt ctctttttaa
     1141 taaagaagaa gcttttttag atgagattga ggcaattgaa atttcagcaa ttaaggaaat
     1201 aggaaatatt atggcaagtt catatgtaaa tgctattgca tctatgttaa acatgactat
     1261 aagtgtttca attccagata tatgtattga tatggttgga gctgttctaa atgtacctat
     1321 gataagattt tcagatgttg gtgacaaagt cttatttata gaaaataaat ttaaaatgag
     1381 tgataattat tttactagtc acattttgat gattccagaa atgagctcac ttagagaaat
     1441 actcgtgagg ttgggattag aagtatgaat agagagatag tagtgaacat tgcagatatg
     1501 aagatagcct atagaccaaa tgttttagtt acttatgcac ttggttcttg tgtaggagta
     1561 tgtttgatag ataaagtagc tggaattggt ggaatgttac acgttatgtt gccatatagt
     1621 aaagatgcta ttaacattga aaataagtgt aaatttgctg acacaggaat aaatgagctc
     1681 attaaatcta tggagaatat aggtgcaaat agaatatata taaaagcaaa aattgctgga
     1741 ggtgctcaga tgtttttagg aagtagcaat tcagtaattg caagtatagg acataggaat
     1801 gttgaggcag taaaaaaaac tcttttagga ttaaatattc caatattagc tgaggacaca
     1861 ggtatgaatt atggtagaac aatacgtttt tacactgaga cgggtgaatt acttgtagat
     1921 tcaataaata aaggtaagaa gaaattataa tatatatttc atagaaagtg tattttagga
     1981 gggaggtatt aaatgagatt tccagatgac ccatatttag attatggact aattgatgaa
     2041 ggaattaagt atctaaagtt taaagtagat gagcaagatt ttggggttga acttgaaaag
     2101 gtaatagaaa ttactggaaa tcaagaaatt atatttattc cagaactacc tacatattct
     2161 aaaggcataa taaatctaag aggaaaaatc attccgataa tagatatgcg tttgagactt
     2221 aaaaaagatg aattaatgac tacaaattgc atgtatatag tagtaactga aataaaggac
     2281 ttggtagttg gatttgtggt tgatagagtt gatgatatag taagcttaga aaaaagtaaa
     2341 atatcacctc caccaagagt tactatagaa tattcggatt attttgtaag tggagttgga
     2401 gaattagaag gtaaaataat tactttacta gatttggaga aacttttgac agataaagat
     2461 gaagttttaa tagatgaatt aatcaatggg tttgattgca ctatcatgta acatagggat
     2521 gtagttggag tgttattata aaaagattgt gatgagaaca tttaagaata tagtggtata
     2581 attattaagt ataaaaattt taaatcatta aaatttgaat atattatgtt taagagaggt
     2641 ttgtatatga aaaataagca acagggaaca attttagtcg ttgatgatag tgttctgatg
     2701 tgtgacttaa tagataaaac acttacaaaa gataatttta aggttaaaaa agcatttaat
     2761 gcaatagagg caaaagaatt tattgaagaa tttataccag acattatttt gcttgatata
     2821 attttaccag ataactctgg ttttgagttt tgtaaggaag taaaatctaa tgctcgtaca
     2881 gcagatatac ctataatttt tataacttca aaaaatacag atacagacat agtaaaagga
     2941 tttggtgtag gagcaattga ctatatggtg aaacctttta gtatgacaga attaaaagct
     3001 cgtgttatag ctcatcttga agtaaagaaa tctcaagata aattaaaaaa gataaataat
     3061 gaactggaat cttctcttga aaaattgaat agactcgttg taagagatta tcttacaggt
     3121 ctttacaata ggcgacatat aattaatcag cttatggagc aaagaagaat taataaatat
     3181 agtgaaacat caatatccct tatccttggt gatattgatg actttaaggt tatcaatgat
     3241 acttatgggc atgaagcagg ggatttcgtg ttaacagtaa tatcaagtat aataaaaaag
     3301 aattgtagac aaattgatac tgtatcaaga tggggtggag aagagttttt gataatgctc
     3361 acaaatactc caatagaagg tgctaagata ctttcagaaa gaatccgtaa agagataaag
     3421 gagtttgatt tcaactataa agaacataaa ataaagtgca ctattacatt aggagtagta
     3481 gaagtaaact caaatatatc attagaagaa aacatatcct tagcagataa agcaatgtat
     3541 gagggtaaaa acttaggaaa agattgtagc gtagtgagta caatgaaagg attaaaaaag
     3601 atataaactt actttatgaa ggagtacaga tatgaaaaga gaagtaaagc tttctaaatt
     3661 agcaattgta ttgctgatta ttatagtatt ggctgttcta gcagggattt tttcttctat
     3721 ttcatcttat aaagcatttg aaaaagtaga aaaaagtatt aatgatatga ataatttgag
     3781 gactcaaagt cagtatataa aagatacaac tagagagata agtgatgtat taagaagata
     3841 tgtatttaca ggagaaaaaa agaataaaga tatttatgaa aaagaattat acggaaaaac
     3901 aagagaatca gctttaaaca aattagaaaa aattggtctt acaaagagcg agtcaaataa
     3961 aatcttagaa gcaaaagaaa tatcagattc tttagtacca acagaacaaa gtgcaataaa
     4021 ggcagtcgaa agtaatgata agagtaaagc tcaaagtata atttttggta gtgactatat
     4081 aaactcccaa gagaaaatgg attctttaat aaatgaattt caagaggata tagttacccg
     4141 ttcaaattcc aaaattgtag aagtaaaaaa agaattacaa agtctaatat taagaacaat
     4201 atttatactt tcccttttaa taatattgac aataatagaa tatacagtaa taaaattaaa
     4261 aatagtgatt cctattaata aagtagaaaa acacttttca agaattgcat ctggggactt
     4321 aagaacaagt atagacgttg aagaaagtaa atctgaaata gggctattag tgagttctgt
     4381 caaaaagatg caaagtatgc ttttaaatta catagacctt atagacagta cattaactag
     4441 tattgcaaaa ggagatttaa gagtagagat agaccaagaa tttataggtg attttaaatc
     4501 aattaaaata tctttagaaa gtataataaa ctcttttaat ggtgattttt ctgaaataaa
     4561 tttatcagca gagcaagttg caagtgcttc agaacaagtt gcagcaggtg cacaagctct
     4621 ttcacagggt gctactgaac aagctagttc agtagaagaa ttagcttcca cgattaatga
     4681 gatatcagat ggaattaaga agtgtgcaga tagtgctagt cttgcatgtg aggtatctga
     4741 ccaatctgca tcacaagttg gttttggaag tagatgtatg gatgatatga tgacatcaat
     4801 gggtgaaata agtgaaaaat cgagtgaaat atcaaaaata atcaaaacaa tagatgagat
     4861 agcattccaa actaatattc ttgcattaaa tgcagctgtt gaagcagcaa gagcaggtca
     4921 atatggtaga ggatttgcag ttgtagcaga tgaagttagg tctttggctg gtaaaagtgc
     4981 aaaagcagct caaaatacag ctattctcat agaagaagct attaaaacag tagaaaatgg
     5041 tgtcagaatt gctaaagaga ctgcaaatgc attggacttg gtagtaaatg gtgtagaaaa
     5101 aactactaat tatattaatg gtatttcttc ggctgtacat agtcaagaag tagcaattac
     5161 acagatttta tctggaatag agcaaatttc catagtagtc caaatgaact cagcaacagc
     5221 agaggaaagt gcagcagcct cagaagagat gtctgctcag gctcaaattt taaaagaatt
     5281 agtagaaaaa tttcatctaa aggaaagtga agtaagagaa tcatcatggg tttaaattat
     5341 gaggtgaata ttgaagtaag agaattatca tgtatttaaa ttatgagatg tatatatatt
     5401 gaaataagag aattatcaag gatttaaatt atgagatgta tatatattga agtaagagaa
     5461 taattgtgta tttaaattat gaggtacata tagtgagata agagaatcat caaggattta
     5521 aattatgaga tgcatatata atagaaaatt atttgtaggt cgtaggtgat aaatgtgaat
     5581 tatattgaac cgagtatgag acaaatgtta gatatgttta tatttgagac aagtttatta
     5641 ctcgaacaat tagatgaaat tatgattcaa atagaaaaag agaaagtatt tactcataac
     5701 aacattaatg aaacttttag aataatgcat acgataaaag gttcagcatc tatgatgggg
     5761 ttagacaata tatctgcttt agcacatagt gtagaagact tgttttatgc tatacgtgaa
     5821 ggtaaaatag aagttttaga caatgaccta ttatttgatt tagtctttca atcattagat
     5881 tttataagaa gagatataga tgagttacaa tcaagaggtt gtatatcttc tgattactct
     5941 aattttattg aaggaattga agagtatgtg agtactttgg aattatcaag ttctatgaaa
     6001 tcaaaggata atgcaaattt agaaagtaaa gacgaaaaag aagtaatcga ttcaaatttt
     6061 gaatacaaag aagactctga tgtagtaaaa ataatctttg aacaaggttg catgatggaa
     6121 aatgtgagag ctttcatgct tgtatttgaa cttagagatt actgtgaatt tatagatttt
     6181 tatccaaaag atgtagaaac aaatgcagag tctacagagt atattaaaag ttatgggttt
     6241 tatatacaat ttgtagctaa agaggataag gcaatagttt ataaaactat agaagataat
     6301 ctctatgtaa aagaatacat aaatattagt aaaaaagaaa tagaaatgaa aagtacatca
     6361 aacaatgatg cagtagaaaa taaattaaaa gataataata aagtaagtaa aagttatcaa
     6421 aataactata cagaagatga gatacaagaa gatgttttaa atgaaaaaac tgatatggaa
     6481 aaagatagtc taagtgaaga aaatgaacat ttaccaataa gtaagcagag tattatgagt
     6541 gtaaatttag aaaaattgga cttacttcaa aatatagttg gagaaattgt aattatggaa
     6601 tcaatggttg taaatagcgt aaattctaca ggtgttgata tagataattt caataaggca
     6661 acaagacaat tacacaagct tacagatgaa ttgcaagata tagtaatgtc tatgcgtatg
     6721 attcaaattg ctggtgtatt tcaaaaaatg catagaattg tacgagatat gaataaaaaa
     6781 ttagataaaa atgtagagtt aataactata ggagcagaaa cagaggtgga taagagtgta
     6841 attgataatt tagcggaacc ttttatgcac cttataagaa atgctatgga ccatgcaata
     6901 gagcctattg aggagcgtat agcgaaaggt aaagaagaag tgggaaagat agttttatct
     6961 gctgaaaatc taggcggaga agctgtgata agtatttcag atgatggtag aggcattatg
     7021 agagataaga ttttggcaaa agctaaggaa caaggtcttc ttaaacatca aagtaatgag
     7081 tatactgatg acgaggtcaa ttctttgatt atgactccag gcttttctac aaaagaatct
     7141 ataactgaat tttctggaag aggagtaggc atggacattg ttcgtaaaag tattgaaaaa
     7201 gttggaggtt caattgaagt aaaaagtaaa caggatgagg ggacaacctt tactataaaa
     7261 ataccattaa ctctttctat agtagagggt atgaagttta aagttggaga ttcactattt
     7321 actttaccaa tatcatccat aagaagaaca tttaaactta cagacttaaa tcaaataatt
     7381 acagaaggaa ataagacaga gttaattaca atatatggag catgtattcc cattataagg
     7441 ttacataaaa tatataatat tcaaacaaaa gtaaccaatc tgatgaatgg taatatgata
     7501 attattgaaa atgagcataa agcagtatgc atttttgttg atgaaataat aggtaatcag
     7561 caagttgttg tgaaaccatt tccagtttat tttaaccgat ttaatataaa aaataagggt
     7621 ttatcgggat gtacaatact tggggatgga agtataagtt taattataga tgcagatagc
     7681 attatagaaa tatattaggg gatttaaagt atggctaaag atgaaattat taaacaagta
     7741 ttaacatttt atgtcaatga tgttatttat ggaatagaat tagaaaatgt gattgaaacc
     7801 ataagatttc aatcaataac gtatgtacca tgtcttccaa catatataag tggagttatt
     7861 aatttgagag gaagaataat accagttatt aatatgcata taaagtacaa tcttcctaaa
     7921 gctgattata atgagagaac ttgtataata attacaaagg ttgatgagta tcaagttgga
     7981 attattgtag ataaagttgt tgatgttatt catatagaaa atctaaattt actagagacg
     8041 actaattcta acaacactaa tattaataaa gatataaaag agattgcgaa agttgaagat
     8101 aattctatat taattttaga tattcgaaag tttttgatta atagcatata gttagttctg
     8161 ataaattagc aggaggaagt ttttgcatga taaaactcac agatgaagaa tttatagttc
     8221 ttgtaaacca tgtaaaaaaa gaatatggta ttgatttaag taaaaaaaga gcgttaattg
     8281 aaggtcgctt atacaataca atgatagaga aaaaacttag ttcttttagt caatatatga
     8341 atctattatt taaagatagg acaggaaatg aagctataaa cctaataaat agattgagta
     8401 ctaaccacac attttttatg agagaacctc aacattttga gtttatacag aatagtatac
     8461 ttcctttttg ggaagaaaat aataaaataa gaaacataaa tatatggagt gcaggatgtt
     8521 ctagtggaga agaagcgtat tcaatagcta tgactttgga tgattatttt ggatataata
     8581 aagagttgtg gaacattaaa atcatagcca cagatatttc aactaactca cttgaaaaag
     8641 ctaaaagagg tatatacata gaatcaaatg ttaacaatgt tcctgagtta tggaagaaga
     8701 agtattttat agacaacaag gatggtacat ttaaaatttg tgataagatt agaaaaagtg
     8761 taatttttaa gccgtttaat ttaatgaatg atttctcata tcaacatttt gatttgattt
     8821 tttgtagaaa tgttatgatt tattttgatt taaaaacaag agaagaactt ataaataaat
     8881 tttataatgc taccaaaaaa gatagttttt tgtttgtagg acatgcagaa gtaatcaaca
     8941 gaagtagtac taaatacaaa tatgtaaaac ctgctatata taaacgataa aaaggtattt
     9001 ttgatatatg attttttgga ggcggtaatg tggaacgttt taataatatg gttattgcta
     9061 taggcgcatc tgtaggtggt acagaggcaa tacttgaaat tataaaagac ctaccaaaat
     9121 caacaccagg gattgttgta gttcaacata tgccagctat atttacttat atgtatgcac
     9181 aacggcttga caagcagtgt attatgaatg taagagaagc taagaataat gatagagtag
     9241 aacaaggaaa tgtgttaatt gcacctgggg gttaccagat gaaattgtgt acggataaac
     9301 aaggatatta tgtaacttgt gaaaaaggag aaagaataag tggtcactgt ccatcagtag
     9361 acgttttgtt tgattcagta gcagaagtag caggtaaaaa ttcaatagga ataatactta
     9421 caggtatggg ttatgatgga gcaagtggac ttttaaaaat gaaagaaaat ggttcattta
     9481 caattggaca ggatgaaaat tcttgtatag tatatggaat gccaatggta gcttttgata
     9541 gaggttctgt tatgttacaa ttacctcttg gtgaaatttc aaactgtttg ataagacata
     9601 ttaatatttt aaaacaaaaa gattaatttt ttataaataa aaattaatct tttattaaat
     9661 tatatggata aaaaacccta gaaactccaa atataataag agagatttat atttaggagg
     9721 aaaatgataa tatgaaattt tttaaagtcg atacaaaagc taaagtttta agatatgtat
     9781 ttgttttaac attgactata atggctgttt tggcaattaa acatgactat aaatataata
     9841 atttaaggaa agatgcttca attaatttaa atagtaataa ggaagcagtg actgttatga
     9901 gtaaccagtc attaccaaag ggtaatacaa atagtaaaca aactttatca gtaagtacaa
     9961 taggaagtaa aacaatagtg aaaaatgatg agtatttaga aagtactata aacatgcctg
    10021 taataacaaa ttcaaataaa attgttgaaa ggtctacaaa tgataagata aaaaatgaca
    10081 tatttgaatt ttataataaa tcatataaag aagcaaagca gtatcttaag gataatcctg
    10141 acgagaaaaa taagtttgtt gcaaatgtag actttgaact taaaaagaat actgattctg
    10201 ctcttagtat aaaagtgaga tattatactt atagtggtgg agcacatgga ttttatcaag
    10261 atatagcata taatgtggat atgagaactg gaaaattttt agaattaatg gacttgttta
    10321 aggataatac taaatataaa gaagttatag atgaagaaat taaaagacaa atagctgaac
    10381 ttgaaaaaaa ggatgaagaa aatatcggaa tatataattt taaaggtata aaagagaatc
    10441 aaaactttta cttgcaagat gagaatctag ttgtatattt tgatttgtat gatataactc
    10501 catatgcagc aggaatacca gaattttcaa taaataaagc gttgataagt tctatgctaa
    10561 aaccagagta tgtagattta tttgatttga aatagaagat taaattaaat gttatgctat
    10621 aatatttagc atagagactt attaaaattg ggggtataaa aatgctactt ttaaaaaaag
    10681 acgacataaa aaaagtattt acaatgagag atgcaattga agctgataag gaagctttta
    10741 gaatttattg tgaaggaaag agtgtaaatc ctttgagaac caatatatct gttccaagtc
    10801 aagatgctag tatgttattt atgccaggat acgtagaaga attaggctgt gggggaataa
    10861 aaattgtatc tgtatttcct aaaaatgctc aaaaaggcaa accagtaatt ccagcaacag
    10921 tactattatt agatggagaa actggagaag ttagtgcagt attggatggt acttatgtaa
    10981 ctcagattcg tactggagca gcctctggag cagctataga cgtattggct aaaaaagatt
    11041 ctaagattgg agctttgata ggtctaggag gacaaggtga gccacaactt gaagccatga
    11101 tagaagctag aaatttggat gaggtaagag tttttagtag aaacaaggaa agtagagaaa
    11161 aatttgcaga agaaatgaac ttaaaattaa gtaaatataa tactaaaata gttgcagttg
    11221 aatcatctga cgaagccata gataacgcag atgtgatagt tttagctact ccatcaaaac
    11281 aaccagtact taatggaaac ttagtgaaaa aaggagcttt aataagtgca gtaggttcat
    11341 atatgccaga tatgcaagag ttgtgcccaa actgtttaac tagagcgtct aagatatttt
    11401 ttgaatcaac agatgcagtt ttatctgagt caggagatat attaattcca ttaaaagaag
    11461 gtaagataag taaagatgat tttagtggtg atttaggaaa tgttataaac ggaactattc
    11521 ctggaagaga agatgatgat gagataattg tatttaaaac tgttgggata ggtgtacaag
    11581 atgtcgtaac agcaaaaaga atatacgata aagcaaaaga aaacggtatt ggaatagact
    11641 ggaagtggta aaatattcaa aataattcta tagttagaca aatttatgtt ataatgttga
    11701 taaataaatt ttttattatt ttaattgagg gaggatgttt gtatgaaaat ggtaaaaaag
    11761 ttaatattat ctacgattat ggctctagtt ttagttctaa ctatagggtg ttctaatgca
    11821 gaagaaaaag taaagtcgaa ttttgaagct aatttagctg cattagaaat gtataagtat
    11881 gatgaaaaag gaatttctga tgcattaaca gaagctgaat ataaacagat tcaaacttat
    11941 aacagcaagg acaaggtttc aatgattgct caattgacac ctctttatgg taaagaaaat
    12001 gctgaaaaga tatacgaaac aattgttaaa ttaattcaag gtgttgaaaa gacagtaaca
    12061 gtggaatctt ctagtaagga taagataact ttgaaagttg taagtaaagc tttagatact
    12121 agtaaagtaa tgagtgaagc gaacacttta gtaaaatctt atgtaactga tgaaaagaat
    12181 tacgataaaa aagaaaaaga agttcaagat gatatgacaa aaatgattat agacctttac
    12241 aataagaaac caactaagga agttacaact atgattacat atactaaaaa agatggtaaa
    12301 tggcaaccac ctagtaatat acaaaagtta tgtgaaggaa tatatacaat ttaatttatt
    12361 ttaaagacac aaaactatct tttaagcata taataaaatg tttggaagat agtttatttt
    12421 attttcaaag tataaattat attttataaa tgatttattt tataaataat ttactttatc
    12481 ttttactata tttaaattta ttataagttt tcttgccaga aatttttagg tgttatattt
    12541 tctgtaattg gcaatattgt atatcctctc tcttttaaga ttcttataat attatttaaa
    12601 gcatttaaac tttgctcttt ctcatgaatt aatactacta attcatctct ttttcttcca
    12661 taatataata tattacttac tatctgactt gtagaagact tccaatcctg tgtatccaaa
    12721 ttccagtccc aaattaaata atcatttgcc ataagtttat tatatgaacc ttctggcata
    12781 tatggtttac ttccgtatgg taatcttatt aattttgaac tctgaccagt tatttcttgt
    12841 agagtattat tgcatgtatt aaattcccca actgttactt ctgggctttt atataactta
    12901 tgtatatcat gacttacact atgaaaacct attgagtttt cttcttccaa gactctcctt
    12961 acttcttcct tgtggttttt catattctga ttaagcatga aaaatgtcgc tttgacatca
    13021 ttttctttta aaatatcaag tagtgcattt gtatattttg atggaccatc atctattgtt
    13081 atgtatgcta ctttttcaga agtagcccct ggaccttttg tttcttcttg agatgctttg
    13141 tatatgattt ctcctaaagt attactttga gaaagcagtt ccagtgtatt ttcacctttt
    13201 gaacttatat tttcaactgg aacattatta attttttcct ccaataatgt tccagttaaa
    13261 attaaaacag ctataagaaa tatccttata aatacatgaa aaacagcctt attatcttta
    13321 attttcatcc cccatatctc ccttttataa aagcataagt ttaagactta aaaagttatt
    13381 ttttatattt attctctttc ttattacttt ttatatctct actaaatatt tttttaacct
    13441 tttcttcttc tttttctcct aaagattcta ctcttttgat tcccaaagaa gttaaaaatt
    13501 gtaatacatt taaattatca aatggagttt ccatattcgt actttcacta tattcatcaa
    13561 cattcttagt accttcttcc acacttaaaa tacgcttcgg acctcctaca cagcctccta
    13621 cacaccccat tccttctata aatgtagctt ctatttcttt atttagaact ttctttagac
    13681 cgtccatgca ctcttttgtt ccttcaaatg ccttttcttc aaattttata tttttgtcaa
    13741 ttcttctaac tgataactta actgctttac ttacacctcc tgttctagca taaactctac
    13801 ctgaaaggga cgcctctact ctattactct catccattat tgctggattt aactctaatg
    13861 catcaaatat ttcttgcaac tctttaaatg taagcacaaa atcaactgca tcttttatat
    13921 cttctaacat tgcctctttc ttttttgcca tacaaggacc tatgaataca actttagcat
    13981 ctggatttaa aatttttaca gctctcccac aagcaatcat aggagaaacg gatggtgata
    14041 cattctccaa tatttgagga aaactatttt gtatcaaact aacccaaata ggacagcaac
    14101 aactagttat aaagcatcct tcttcgtcct ttttcatatg ctcataataa tcatatgctt
    14161 cttttgcagt aagcatatct gctgccaaag caacttctat catatcttca aatcctatct
    14221 tttttaatgc agttcttaat ttaccagggc ttacatcttt cccaaactgt cctacaaaag
    14281 ctggtgcaac tattgcatat acagggctct ttatatcttt taacaatttt atcattggta
    14341 taaattctat tttatctgaa attgcaccca acttacatct tgatacacaa tcaccacaag
    14401 cactacatac atcttcctca ttacaattaa tatgcttact tcttatctta catgactcat
    14461 cttccttatt attttcatca caatatttgc aagcatctga tattacagat attattggtc
    14521 ttttgacttc atctaaattt acagcttcat gtagtgactc ttttaaattt ttatctgtat
    14581 cttctgtcac atccaatccc atagttaccc taattaaatt taccatagta tctttatcaa
    14641 attttccact aaaataatta tttaaattat cctttaaatt ttcatttaaa attttattag
    14701 gtaagttttc aatatcctct atgtcatcat tccacactgc ttttacaagt tcgccaaaaa
    14761 gcttcatttt atatatttct agtattttag aattgtcatt tctagtattc ataaaaaata
    14821 gtacctccat aaattaaact tatatatgtt taatatttac aaagatacta ttttttattt
    14881 attatttaac tactattatt aatatgattt atttagtttt ctcaacaact ctatttcaat
    14941 cattattttc attagatgat agttgaatac agggcttttt tcttcatcta tattccagtc
    15001 tagcttatcc acattatatg ttttcttaag gtatttatat gtatgctctt tcaaataaag
    15061 tttattttca agtaactcta tagactgcat atgaaaatgt atttctctac atatccttaa
    15121 ggcattctca attttttcta tattagtttc actcaaacta taatccaact catcaataag
    15181 ttttgaatat atttcttcta atttttctac ttcttcttca aatttctcta catttaaatt
    15241 ttcttttcta agaattttat cttcaataag caacattgaa agttcttcta tctttttaaa
    15301 ctcatctata acactattta gataatttgg tctagcaaca taataattaa caagtactcc
    15361 aaacaaaaca cccacagatg tatctaacac cctatgtaaa gaataataca cagggtcact
    15421 atttcctaca cctagatgta tagccagaaa ggttacacag gcaactacta ctgactttat
    15481 tttaaacaga ttgcataaat atattgagca cattactcct agaccacata aaaatgggtc
    15541 tccagcttta atgatggcaa atatgaatcc tattatacca cctaaaatag taccttccac
    15601 tctattgatt cccgtcttta aagaaccttt tactgtatct tgaacagaaa ttatacatgc
    15661 taccagagaa taaaacatat tctctactaa atagtcacca agaatcatac ataaagtgac
    15721 actaattcct gtttttatag tcctcatacc catcttttga agtttcaaag ttccacctcc
    15781 taaaaagcac cttatataaa atacgcatcc tcatatttaa tatgttccta gtatatcaca
    15841 attttttaaa tttacccatt aatcatattt ttttatcaat tctttcttaa tttagacaat
    15901 aaaaaaactg ctttaaagca gttttttatc ctatctcaac cattttaccc tcaattgcaa
    15961 aaattatctt ctcacataca tttgttatat ggtctccaat tctttcaagg tatcttccta
    16021 taagtagaag ttttacccct tggtctatgg tatttgagtt ttcatgcatt atttttaaac
    16081 aatcaatttg tacttgttca tacaatccat ctacaatatc atctttagtt ccaacatcat
    16141 aagctatact tgcatcaaat aagattaaag cttctctagt ctgagacatc atagacttac
    16201 attcttttgc catcttagga atatctatta gctcttttat ataagcctct ccacctatct
    16261 tcaacatttc tctagctata tttgctgcat aatctcctat tctttccagc tctatagata
    16321 tattacctaa tgaataaata aatcttaagt cccttgccat tggttgcttt aatactatta
    16381 gctctatact cttatctctt atttcttctc ttaatttatt tatttcttca tcatcttcta
    16441 ctacttttct tgcaccctct aaatccttct ctatcataca agtcacagac ttttctattg
    16501 cttcttcaca tttttccatc ataagcactg tttgttcttt taaagtatca atacttatat
    16561 ctaagctatt attgaccatt gtctaactcc tttatagtta tatattttaa ccaaatctac
    16621 ctgttatata gtcttctgtt cttttatcta caggagtggt aaacatagtt tttgtatcgc
    16681 taaattctat aacctctcca tttaagaaaa aagctgtttc atctgatata cgagcagctt
    16741 gttgcatatt atgtgtaact atgactattg tgtagtcttt ttttaaatcc tctataagtt
    16801 cctctacttt taatgttgaa attgggtcta aagcagatgt aggttcatcc ataagaataa
    16861 cttctggtct catagcaatt gctcttgcta tacatatacg ttgttgttga ccaccagaaa
    16921 gtcctagtgc agatgattta agcctgtctt taacctcatc ccaaatagct gcacctttta
    16981 agctttcttc tacaatttta tctaattgct tcttatctct tagtccatgt gtacgaggac
    17041 catatgccac attatcatat atactcatag gaaatggatt tggcttttga aataccattc
    17101 ctactttagt acgaagattt ataacatcat ctgaggtata tatgtcctct ccatcaacag
    17161 aaatgtttcc tttaattgtt acatcttcga ttaagtcatt cattctattt aacgttctta
    17221 taaaagttga ttttccgcat ccagatggac ctattaaagc agtaacctta ttctctttta
    17281 tatccatatt tattttcttt aatgcttgct tatcaccata aaataaatcc aaatctttta
    17341 cactcatttt tatcttatcg attaattcca ttattcacac ctcttaaatt taataattag
    17401 ctttatttaa tttcttagca acaaatcttg ctagcatgtt tagtgctaat acaattacta
    17461 ttaaaacaat tcctatactg gctgctgttg ctatatctcc actctcttta gtcagtaagt
    17521 atgagtgtac agttaatgtt cttgcactat caaatattcc acctggcatc ttggctacag
    17581 tacctgctgt aagtaatatt gcagctgatt ctccaattat acgaccaact gataagatta
    17641 ctccagataa aattcctggt attgcacttg gaagtactac cttatataaa gtttgaaatt
    17701 ttgtagctcc taatgccaat gatgattctc tataagcttg aggaactgtt tttaaagctt
    17761 cttctgttgt acgaattatt actggtaata ttattattgc aactgtaagt gaacctgcaa
    17821 ctatggaata tcccattttt aatgtaacta caaagaaaat tcctccaaat aatccatata
    17881 ctatagatgg tatccctgat aaactttcag ttgcaaatct tatagctgta actgcttttc
    17941 cttttttagc atattcttgt aaatatatag cagccaatat tcctatagga gttgctacaa
    18001 ctatagaaac tactactgta tataatgttg taattatcat tggaagtata ccaccatcac
    18061 ctgatgctga ataattgcta aataagaaat taaaattcat gttgccaatt ccctttataa
    18121 atataaatcc cactataact actaatgata ctattgtaaa tagtgctgat agatatacta
    18181 atgacttcaa tagattctct ttgaactttc tcatcttaat ttaccgcctt atttgaaagt
    18241 ttatttaaaa ctaagtttaa aattaatata aatgtaaaca atactactcc agttgcaaat
    18301 agcatttctt gatgtgtacc aaatgcgtat cccatttcaa gtgctatatt agttgtaagt
    18361 ggtcttacac tatctgttat tgcagttggc ataactggtg agttgcctgc aacaagtatt
    18421 actgccattg tttcacctaa agctcttcct actcctaaca ctactgcggc taatactcca
    18481 gattttgcag caggtaatac aactttgaat attgtttcta tttttgatgc accaagtgct
    18541 aatgaacctt ctttgtatgc ttttggtact gctcttattg ctgtttctga tacagatatt
    18601 attgttggta acatcattac tgctaatact attactattg ctaacaaact ttgacctttt
    18661 ggaagattaa atatactttg tatagttgga actatcactg caagaccaaa tacaccatat
    18721 aatactgaag gtatacctgc taaaagttca actgctggtg acattatttt tgctaatctt
    18781 ttaggtgcta cttctgctat aaatacagag gttaatatac caacaggtac tcctataaca
    18841 agtgctccaa gtgttgcaaa tattgatgct actatcatag atgatattcc aaatttattg
    18901 gcagaaggaa cccaatctgc accagttaaa aagtctataa atgaatatcc ttcaaatata
    18961 aatggtctta atcctttata aaatacaaat cctattatca aaagtaaact tgcaactgct
    19021 actaaagcac taagtagaaa aatatttttt gctacttttt caattacgta ttttgtctta
    19081 ttgccattat tgtcattttt ttctaaacct actaatctag attttactat cataataccc
    19141 tcccattaaa atatacttat cgattttcgt tctgtattca aaatttaatt ttttataatt
    19201 cgtcttcatc aaatttcctg tacttttatt ctactttttt taagttaaat ctatattagg
    19261 attaagttaa gcaagtgtaa aattatattt actttttaac attaatttta cttttaaaac
    19321 tatatttgac atttacttta caatatactg ttgtttctct atattaaaat agcagatata
    19381 gataataata cagtttatgt ataatttatt acctatatct gctattaaga tttgttaact
    19441 aaatttaact tttatactac atatataatt attgtatcct ctagtttata tcactattat
    19501 ttccatcaaa ttgttacgat acttgatgtt tgcaataggt atgaatagcc aaaaatataa
    19561 aaataggtat ttctatcact gagaaaaaca gtataatgtg tggtgtccaa aaactcatac
    19621 ctcctaagtg taagaaatta aatgatgtca tttgatataa taaactaatc ttaaaaccaa
    19681 ttccccctgg cggtaatagg tatccaatcc aactaacacc tattcccata ggaattaagc
    19741 aaatagaaaa agcaatcagc atagaagtaa gagaatcctt acattttgca gataaaaata
    19801 atgttaaact aaccattgca agtattgaaa ttagaccacc aatagctata agaatctgtg
    19861 cttgtagtaa atttatattt actagactaa tgacagaaaa caacatttga aatgaggttt
    19921 tcaaacattc agtaccaaaa gctaaatcag aaattgccaa atagatagaa atacatataa
    19981 caaacattat tataaatata gaaaatagag ctaatatttt agtaaacgct agacaaatat
    20041 gtccatgttt cgtacaacga aggatgctat ctgattgtgt ctgatactca ccagaaaaag
    20101 taggtgcagc aattgcaaca tatataatta ataatataaa aatataaaat attatataat
    20161 cgaaagcatc ttctggattt attaagaaat tcttaat
//
LOCUS       DAMJYR010000061        19892 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.061, whole genome shotgun sequence.
ACCESSION   DAMJYR010000061 DAMJYR010000000
VERSION     DAMJYR010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 19892)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19892)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19892
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.061"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            356..1147
                     /locus_tag="PC471_002221"
     CDS             356..1147
                     /locus_tag="PC471_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamB/YcsF family protein"
                     /protein_id="HDF2789349.1"
                     /translation="MYKVDLNSDLGESFGTYKIGLDEEVLKYISSANIACGFHAGDPS
                     HMEKTVQLAKKNGVKIGAHPGFLDLIGFGRREMKITKQEAKDYTKYQLGALMAFASSN
                     GCNIQHVKPHGALYNMAAKDKELAMGICEAIYEVDKDIILLGLYNSEMINSAKEIGLR
                     FANEVFADRAYDNNGFLVPRNVEGAVIHDTKHAIDRVVRMVKEGTVETLTGEVIHIKA
                     DSICVHGDNPKAIEFVKEIRKRFELEGIEVCPLENIEVCSSENIV"
     gene            1176..2396
                     /locus_tag="PC471_002222"
     CDS             1176..2396
                     /locus_tag="PC471_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HDF2789350.1"
                     /translation="MSTKNITDKTKNKKDVGALIGAAFIMATSAIGPGFLTQTAQFTQ
                     DFGPNFSFVILITTILFIGAQVNVWRVIGVSGLRGQDIANKIIPGLGYLVAFLVALGG
                     LAFNIGNVGGAALGMNVMFNMNMTLGTVLSGLIAIFVFMSKNSNSLVDKITKFLALGM
                     IIIVGYVAISNHPPVGEAVSRMVKPENPKGLIFPIITLLGGSVGGYITFAGGHRLIDG
                     GITGEENIKEITKSSLLGILVATMMRVLLFLAILAVVSKGLQLDPENPAASAFKFSAG
                     AIGYKFFGLVLWSAAITSVIGAAYTSVSFLKTLNPFIDKYEKYFIIAFIAISTLIMAF
                     IGKPATLLILAGALNGLILPITLGIMLIASKRKDIVGDYKHPTWLLIFGLIVVLISAY
                     TGITSLSSLGALFA"
     gene            2416..3111
                     /gene="pxpB"
                     /locus_tag="PC471_002223"
     CDS             2416..3111
                     /gene="pxpB"
                     /locus_tag="PC471_002223"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HDF2789351.1"
                     /translation="METRYLISGDKAVVAEFGNEISEDINKKVISFMRAIEISNLKGI
                     VTEMVPTYRSLMISYNPLEIDFDSLIENLKKIEDNLESIVLPKPKIHEIPVCYDEVFG
                     IDIKNVASYNNLTVDEVIKIHTSREYLIYMLGFTPGFPYLGGMDERIATPRLEVPRTK
                     IYGGSVGIAGSQTGVYPIDSPGGWQIIGRTPLKLYDENREEQILLRAGDFIKFVPITL
                     DEFIEIEKNNLSI"
     gene            3141..4127
                     /locus_tag="PC471_002224"
     CDS             3141..4127
                     /locus_tag="PC471_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-dependent carboxyltransferase"
                     /protein_id="HDF2789352.1"
                     /translation="MGFKVELGGFQTLIQDRGRVGYGQYGVSGCGAMDEYAHRVGNIL
                     VGNSEDEASLEVLMLGPTITFDEYTQIAVTGGDLGAKINGKEIQNWRSYQINPGDVLS
                     FRGVKSGTRAYVSIAGGIDVPLAMGSKSTYTRAKIGGFEGRALKKGDYINTFIQEKDF
                     TINKKLSSKYIPTYSSEIVLRIVKGPQFDAFSNGEVEKFLSNKYKVTNEIDRMGCRLD
                     GESIKHLNGADIISEGISYGAIQVPGHGKPIIMLSDRQTSGGYTKIGNVISVDLYKLA
                     QAKPNDVVKFELVDIYEAHRLLREQEDKIQDIYKSMKNIRVVKAKVLNDIAV"
     gene            complement(4341..4907)
                     /locus_tag="PC471_002225"
     CDS             complement(4341..4907)
                     /locus_tag="PC471_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2789353.1"
                     /translation="MLGEKMRNIRKSKKKTLSDVSKLTDLSISYISQIERDAIEPSLS
                     SLRKIAEVLDTPLYMFMDDNNTDDLVIRKEDRVMMKFPKSEMFYEIVSPMPTTEFTPS
                     ILFVEFELKPESEDAKDYIYHASEEIMVVTSGVVDICMGEKVVRLNPGDSTFIKANVP
                     HKIINPSKDTIARGYGVISPPIWPIKSK"
     gene            5259..6326
                     /locus_tag="PC471_002226"
     CDS             5259..6326
                     /locus_tag="PC471_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide epimerase"
                     /protein_id="HDF2789354.1"
                     /translation="MKITDIKFEKLRIKLKKPVVVSFGVIEYGESIILKIETDEGYCG
                     FGEAAPLAAVTGEVLDNVLSVLLMFKKELIGKDPLDIETIHTIMDGIIIGNTSAKAAI
                     DIALYDIKGKIMNAPLYKVLGGFDSKVQTDITISIDKPEKMAVEALERVKEGFRILKL
                     KAGINPEDDIEAVKLIREAVGESIRIRIDANQGWNVNSSINTIKKLEEFNVDAIEQAL
                     PHWDLDGTAYIRNKSNTKIMIDESLHSPIDAIKAIKKNAVDTFNIKLMKSSGIYPAIK
                     INNIAEASGVNCMLGCMLETRIGITAAANLIASKKNITEADLDSFMFCEELEGISGGF
                     VMDGDIMNLVNKPGLGIEVNL"
     gene            6345..7505
                     /locus_tag="PC471_002227"
     CDS             6345..7505
                     /locus_tag="PC471_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF819 family protein"
                     /protein_id="HDF2789355.1"
                     /translation="MNTLFSNPGSILAVMTSMIALGFYLQRYKAIKSLGPALTIIIMG
                     IILSNLKVVPVSTELYGTISTYAIPVSMTIMLMSVDLKEMTKLSREPLIAIFVAVLTV
                     SIMAFLFGLVFAEKISEGWKVAGMFVGTYTGGSANLTAIGTGLNVSRQTLAAANAADY
                     VIGVPTLIFMFALPAILKNSKKFKKLWPYHVEESELEDCQNEEFMESKEWSIKDIAWM
                     LAIGFVVTEVATILAGYFNSSFSSAARILLVTTISIIIAQLKPVKKLKGNLDLGLFVA
                     LFFLCTIGFSVDIKEFLGSTFTITLYCFSIIFASFVFHLGITRLLKIKYQYVILSIVG
                     AIADGPTSALVAASAKWNSLVSVAVVMGVIGGVLGNYAGISVAYAIKMSLGL"
     gene            7518..9614
                     /locus_tag="PC471_002228"
     CDS             7518..9614
                     /locus_tag="PC471_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HDF2789356.1"
                     /translation="MIKFKLYISGLYSGNVIFDGDLLIEKLNPFTNKIESLKPISKEE
                     NTYYLNLTKIDLKSLFNNFDVYTKSLVNTDKDTVINNLGETHSKLNEYIWVQRNKKFP
                     LDIIIVDNKIVGFICLSRETCTILIMDGYEEYTVLKEWEKTHKNEEIYSIRFGGNYMI
                     DMKDGIKLSTDVYLPDFVDSTKKAPTILMRTPYGKENDKEIYYKYVQRGYAVVIQDVR
                     GRNESEGKWEPMIHEREDGDSTINWIVSQEWSSGIVGMLGASYLGYVQWAAASSGNKH
                     LKALVSIVTSGSPFIDIPRKGGAFVSGMLAWAFMVSRNKVDRSKMVRDDWDDVLNIRP
                     IENIPVEALGYRIEFLEEWLKRVEKDEYWDLMDWHLQKDKINVPALVVSGWYDDNSMG
                     TTEALDVIKDYEKGKRKAILGPWMHNSNTLRDINGISLGNSSLRYDLDYNYLLWFDKY
                     LKGIENNIDTTAPVEYYSVGSNKWKTEENWPIINKIDKSMYLISDGNANTSLGNGRLV
                     FDNGLEEKYDSYIYNPKDPSVQLIDMSENEVGVPNNYKDLEKRSDMLCYTSDAFSEEF
                     TVTGDIKLEFFASSSAKDTDWVIKIMDVDLDGNSIKLADGILSARFRNSFYKSEFMEE
                     GEIYKFTIITSKISNTFKVGHKIRLDITSSAKNFIFQNSNTTEGYNSIEYIEAKNTIY
                     HGGKYPSKLILPIENK"
     gene            complement(9773..10117)
                     /locus_tag="PC471_002229"
     CDS             complement(9773..10117)
                     /locus_tag="PC471_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HDF2789357.1"
                     /translation="MEIKRYEGTGRMSRAVVHNNTVYLCGQTHAEGGVIEQTTEVLAK
                     IEDLLNKYGSDKQHLLSVTIYLRDMKDFEAMNSVWDAWVEKGFEPARACVEARLAREH
                     LLVEMSVVAATK"
     gene            10409..11299
                     /locus_tag="PC471_002230"
     CDS             10409..11299
                     /locus_tag="PC471_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HDF2789358.1"
                     /translation="MSSTIKSNRIKGIMFIIASALGFAMMSAFVKLAGDLPSFQKVFF
                     RNLVSAIIAFWLIIKHKGSLTGKKENLKVLLYRSIFGTLGVIFNYYAIDKLMLSDANM
                     LNKMSPFLVVIFCAIALKEKINVKQIGAIIIAFIGALFIIKPTFSVEIIPYLGGIAGA
                     VFAAMAYTCLRVLGDREDYYTIVFFFSVFSLVTVGPIAFAVYEPMTLMQLIYLLLGGV
                     FASLGQFGITLAYKYAPAKEISIFDYSNIIFSAILSIFLFNVYPDMLSVVGYLIVFSA
                     AFYMFLYNKKLDKLDKEKKK"
     gene            11517..12803
                     /locus_tag="PC471_002231"
     CDS             11517..12803
                     /locus_tag="PC471_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDF2789359.1"
                     /translation="MEKVKVAVIGAGNRGTYAYAPYIYENSDVCEIVAVAEPKKGRRE
                     LFTQKYNLDSKNVFETLEDFFKHDKMADAVIIATNDDRHYDVAKLALEKGYHVLLEKP
                     MSNSLDGLVHIDDLCDKYKDKIFMICHVLRYSPFYNKLKEIVESKKLGELVSIQYNEN
                     IGYWHFAHSFTRGNWRNSNETSPLILAKSCHDMDILLYLVGSRCKKISAFGSLKHLNN
                     QNASGEMAQNCLQCLVEKKCPYSAKRIYLEKDRSINRAVHINPTEENLLNILKTSPYG
                     RCVYRCDNNVVDNMVNILQFENGVTATFNLCAFTKENGRTIKLMFSHGEVGGDLNKNE
                     IRIKEFGKNEEIVMNPSNQQNMVEEYDRNLIAEFIKLVSNKELEKGRVAAKEAIQSHV
                     MAFAAEYSRVSDEVVYIEEFFDSAKQMTKEIEETIF"
     gene            complement(12892..13335)
                     /locus_tag="PC471_002232"
     CDS             complement(12892..13335)
                     /locus_tag="PC471_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory YrvL family protein"
                     /protein_id="HDF2789360.1"
                     /translation="MIKISNKRRIPLGEFISVFLFFAVFFGITFCIFTVIGIGFLSFL
                     GFEYKSLGAVLIFFLIYFCITTPIDFLCTTILDIFRYVNKLPYSIYKLCEFIIDFILT
                     FLAMNIIDTFMDSVTIPLSTEILFALLSYLLSECMDFFDRDKKKP"
     gene            complement(13359..14153)
                     /locus_tag="PC471_002233"
     CDS             complement(13359..14153)
                     /locus_tag="PC471_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M55 family metallopeptidase"
                     /protein_id="HDF2789361.1"
                     /translation="MKLYLSADIEGTCGIVNWDETKLECEFSQHYRTQMSKEITAACD
                     GATQSGVHEILVKDAHDSGRNINPNILPENVKILRGWTKDPLVMMAGIDESFDACIFT
                     GYHSGATCNGNPLSHTMDIDYDYFKINDEIASEFTINAYIAAYYNVPVAFLSGDEMLC
                     ESAKKLNPNIVTVPVSKGIGNGSISIHPNLALKKIEKGVKKALSGDLSRHLIKLPDKF
                     KIEIKFREHYKAFKASFYPNMKKIDSQTVLFETEDYYEFLRMLLFI"
     gene            complement(14718..15194)
                     /locus_tag="PC471_002234"
     CDS             complement(14718..15194)
                     /locus_tag="PC471_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HDF2789362.1"
                     /translation="MKEILINPIGKICIQGEEVFIKLDKKYVPALNELDDFSHLNVFW
                     WADGFDNPELRSILETPKPYKSGPDVIGIFATRSPIRPNPIALTAVQIINIDHENGII
                     KIPYIDANDNSPVIDLKPYTPSVDRVENPSIPKWCSHWPKSVEKSGEFNWEDEIEF"
     gene            complement(15295..16464)
                     /locus_tag="PC471_002235"
     CDS             complement(15295..16464)
                     /locus_tag="PC471_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HDF2789363.1"
                     /translation="MLNIKTLCHEINDWVINIRRDLHKTPELGLEEFQTKKKIIKYLN
                     EIGINYIEYKNHTGITAYINVSPNFETVAIRADIDALPITEELNYSYKSINIGKMHAC
                     GHDAHTAILLGTCNILFKLKDYLNVNVKFFFQPAEETIGGAQLMIEDGCLENPNVKYI
                     FGLHVNSNINKNLIELKYNTLNASTDTLQLTVHGSKCHGAYPHQGIDAIVISAHIITA
                     LQTIVSRNTNPTDSVVISLGKIEGGIKENIVCDKVVIRGTLRTLTPETREFSKKRIRE
                     ICDFTCKTFGGSISVEIEEGYPALINSNHLVDYVKQNAVELFGEENIILKDSPTLGAE
                     DFSYFLRHCEGAFYHLGCANREKNITSPLHTSTFDIDEDCLITGVMLHVKNVLSF"
     gene            16627..17490
                     /locus_tag="PC471_002236"
     CDS             16627..17490
                     /locus_tag="PC471_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HDF2789364.1"
                     /translation="MLGIIAPSGPLRNTSLEEIKFNLESYGYEVKFSESCSLNYKGYL
                     AGNDDIRARDIEDMFLDKDVDIIMCLRGGYGTTRILDKINYDIIKQNPKPFIGFSDIT
                     GLNLAFYKNCGLLPYHGIMAANVGKWDKFTYKSLVNALEFKDELYLENPKEEKIYTVC
                     EGKAEGIIMGGNLSLIIATMGTKYEIDAKDKILFIEEIGEPQYKLDRMLTQLYSSGKL
                     EECNGIIFGDFKDCIEENDLMELLIEFANKVNKPSIYNLQSGHCIPMITIPLGRMCEL
                     DATEKIVKLKK"
     gene            17561..18154
                     /locus_tag="PC471_002237"
     CDS             17561..18154
                     /locus_tag="PC471_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3867 domain-containing protein"
                     /protein_id="HDF2789365.1"
                     /translation="MSDDRIIDFNELKNKVKDSDVDKFEQYIYNLYFSVMDGKMSMAE
                     FSRKIFDYMRDNNISQEKFMKIQKQFMERYGMDTEEVEKQLRNFGIDPSTAGFMSNNT
                     SSKVSTEDLESFKKSAGFYEKYGEKIQPKSCITTFIKNDLNDINVIIDQEKIMLCSDR
                     KINLMDSELNEFLLEYKNMFNKKIKVVMCETTNKYDY"
     gene            complement(18405..19040)
                     /locus_tag="PC471_002238"
     CDS             complement(18405..19040)
                     /locus_tag="PC471_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HDF2789366.1"
                     /translation="MDNLLNELEALKSNLDNLPIEKIENYDLSKTALFIIDVNNGFAR
                     QGALYSPRVESLIKPIEMFTKKISNKLNRVIAFTDSHTPKSIELLSYPVHCLENDVES
                     ELVDELKSIENLQILPKNSTNGFFALENLDFDNIDNIIIVGDCTDICIYQFAITLKSY
                     FNQHNIEKNIVVPMNLVDTYDIPNVHPAEILNLVFFNSMIQNGVNVLKEIR"
     gene            complement(19120..19851)
                     /locus_tag="PC471_002239"
     CDS             complement(19120..19851)
                     /locus_tag="PC471_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HDF2789367.1"
                     /translation="MNIYAHRGFSGKYPENTILAFKKCLDMDIYGIELDVHRTKDGKI
                     VVIHDEKVDRTFNGHGFVKDFTLRKLKTLNSSFEGYQSNKECKIPTLEEVLILISPTD
                     LTLNIELKTDKINYPNIEKGVLELILKYNMKNRVLISSFNSNSLKNFHKLDPSVKTGL
                     LCYLPINNVVNFAKFLGNSYLHPPLVLVNESLIELCHKNLLGVNVYTVNEEDDILHCL
                     KLNVDGIFTNYPDIASNLLHSKQYS"
BASE COUNT         7563 a         2334 c         3327 g         6668 t
ORIGIN      
        1 gaaagaaaca aatgaatgaa gaaaaatgat tctgtcttgc atataaagtg attaaaaatg
       61 aaaaatactt taaaaatgaa ggttaaagcc cttattttaa gtgaaaataa ttaattatta
      121 actaaaaaat ggtaaaaaga ttttttgaaa aagaaataca aatgaatgtt caattaaaat
      181 aattgcaaaa atattcctta aatggtaaaa ttatattaat taaatgcaat agaatagttg
      241 caaaaataaa attgaataca agtgataaaa tatttcatgc aatatataaa ataaacaaaa
      301 tttaaaaatt aaaactactt attctatcaa aatgttagcg aggaggggtc ataaattgta
      361 taaagtagat ttaaatagtg atttaggaga aagttttgga acgtataaga ttggtttaga
      421 tgaagaagtt ttaaaatata tttcatcagc caatatagct tgtgggtttc atgcaggtga
      481 ccctagtcat atggaaaaaa cagtacagct agctaaaaaa aatggagtga aaataggcgc
      541 acatccaggt ttcctagatt taataggatt tggaagacgt gagatgaaaa taacaaagca
      601 ggaagctaaa gattatacaa aatatcaact gggtgcttta atggcatttg caagttcaaa
      661 tggatgtaat atacaacatg taaaaccaca tggagcatta tacaatatgg cagctaaaga
      721 taaagagtta gctatgggga tttgtgaagc tatatatgaa gtcgataaag atattatact
      781 gttggggcta tataatagtg aaatgataaa ttctgctaaa gaaattggac ttaggtttgc
      841 aaatgaagta tttgcagata gagcttatga taataatggt tttttggttc caagaaatgt
      901 agaaggagca gttatacatg atacaaaaca tgcaatagat agggttgtta ggatggtaaa
      961 agaaggaact gtagaaactt taacaggtga agttatacat atcaaagcag attctatatg
     1021 tgtacatggg gacaatccaa aagcaataga gtttgtcaaa gaaataagaa agcgttttga
     1081 attagaaggt atagaagttt gtccattaga aaacatagaa gtttgttcgt cagagaacat
     1141 agtataagac tgataacatg agggggagat aaaatatgag tacaaaaaat attacagata
     1201 aaactaaaaa taaaaaagat gtcggagcac ttataggggc tgcatttata atggcaacat
     1261 cagcaattgg accagggttt ttaactcaga ctgcacaatt tacccaagat tttggaccga
     1321 atttttcatt tgtaatttta attacaacaa tcctttttat aggggctcaa gtaaatgtat
     1381 ggagagtaat tggtgtatca ggacttagag gtcaggatat agcaaataaa ataatacctg
     1441 gattaggcta tttagtagct tttttagtag ctcttggagg attagctttt aatataggaa
     1501 atgttggtgg agctgcactt ggtatgaatg taatgtttaa tatgaatatg acattaggaa
     1561 ctgtattaag tggattaata gctatatttg tatttatgtc aaaaaattca aattctctag
     1621 tcgataagat aactaaattt ttagcattag gaatgataat tatagttgga tatgtagcaa
     1681 tatcaaatca tcctcctgtt ggagaggcag tatcaagaat ggtaaaacca gaaaatccaa
     1741 aaggactgat attcccaata ataactttac ttggaggaag tgttggaggt tatataactt
     1801 ttgctggagg acatagatta atagacggtg gaataacagg agaagaaaat ataaaagaaa
     1861 taactaaatc ttctcttttg ggtatattag ttgccacaat gatgagagtt cttttatttt
     1921 tagcaatact ggcagtggtt tcaaaaggtc ttcaacttga cccagaaaat ccagcagctt
     1981 cagcttttaa atttagtgca ggagcaatag gatataaatt ctttggactt gtattatggt
     2041 ctgctgcaat tacatcagtt ataggagctg cttatacatc agtttctttc ttaaagacac
     2101 taaatccttt tatagataaa tatgaaaaat attttataat agcatttata gcaatatcaa
     2161 cacttataat ggcatttata ggaaaaccag ctactttatt aattttagca ggagctttaa
     2221 atggattgat acttccaatc acattaggaa ttatgcttat agcatctaaa agaaaagata
     2281 ttgttggaga ttacaagcat cctacatggc ttttaatatt tggattaata gtagttttaa
     2341 tatctgcata tacaggaata acttctctta gcagcctagg tgctttattt gcttaattta
     2401 gaagaggtga catttatgga aactagatac ttgatatcag gggataaggc agtggttgct
     2461 gaatttggaa atgagatatc agaagatata aacaaaaaag taatttcatt tatgagagca
     2521 atagaaatat cgaatcttaa aggtattgta acagaaatgg taccaacata taggtcactt
     2581 atgataagtt ataatccatt agaaatagat tttgatagtt tgattgagaa tttaaaaaaa
     2641 atagaagata atttggaaag tatagtatta ccaaaaccta aaatacatga gattccagta
     2701 tgttatgatg aggtttttgg tatagatatt aaaaacgttg caagctacaa taatctcact
     2761 gtagatgaag ttataaaaat acacacaagc agagagtact tgatatatat gctagggttt
     2821 actcctggat ttccatatct aggtggtatg gatgaaagaa tagctacacc tagattagaa
     2881 gtgccaagaa ctaaaattta tggtgggagt gttggaatag caggttccca gactggtgta
     2941 tatcctattg atagtcctgg tggatggcaa ataattggta ggacaccttt aaaattatat
     3001 gatgaaaata gagaagaaca gattttgctt agagctggag actttataaa atttgttcca
     3061 ataacattag atgaatttat agaaatcgaa aaaaataact taagtattta aatatcgtta
     3121 tagtacaggg agtggtatat atgggattca aagttgaatt aggaggtttc caaacattaa
     3181 tacaagacag aggtagagtt ggctatggtc aatatggtgt atctggatgt ggagcaatgg
     3241 atgagtatgc acatagagta ggaaatatat tagtaggaaa ttctgaagat gaggcttctt
     3301 tagaagtttt aatgttaggt cctacaatta cctttgatga atatacgcaa atagcagtaa
     3361 ctgggggaga tttaggggct aaaataaatg gaaaagaaat tcaaaactgg aggtcatatc
     3421 aaataaatcc aggtgatgta ttatcattta gaggtgtgaa atcaggtact agagcctatg
     3481 tgtcaatagc tggtggaata gatgttccac ttgcaatggg aagtaagtca acttatacaa
     3541 gagctaaaat tggtggattt gaaggtagag cacttaaaaa aggcgattat ataaatactt
     3601 ttattcagga aaaagatttt acaataaata aaaaattaag ttcaaaatat attccaactt
     3661 atagttcaga aatcgtacta agaattgtaa aaggtcctca atttgatgct ttttctaacg
     3721 gtgaagtaga gaaattttta tcaaataaat acaaagttac gaatgaaata gatagaatgg
     3781 gttgtagact agatggagag agtataaagc accttaatgg agcagatatt atatctgagg
     3841 gtatttctta tggagctata caagtacctg gtcatggaaa acctattata atgttatcag
     3901 atagacagac tagtggtggc tatactaaga tagggaacgt tataagtgta gatttgtata
     3961 agttagcaca agcaaagcca aatgatgtag taaaatttga acttgtagat atatatgaag
     4021 cacatagact attgagagag caagaagata agattcaaga tatctacaag agtatgaaaa
     4081 atataagagt tgtaaaggca aaagtattaa atgatatagc agtttaattt taacaaatac
     4141 aatttttatt atagtttata atataactta caaatagcta tttactattt accataatta
     4201 agatggttta gattagtcaa atagctattt ttttagtttg agtaaatatt ttaaatttaa
     4261 ataaatactt caattttaaa ataaaagcaa tatttaaatc tattatatta tctacaaaaa
     4321 gtaggtgtgt catattgagt ttatttagat tttattggcc aaattggagg tgatataact
     4381 ccatatccac gagctattgt atctttactt ggattgatga ttttatgtgg tacattagct
     4441 tttataaatg ttgagtcacc tggatttaat ctaactacct tttcacccat acaaatatca
     4501 accacaccac tcgttaccac cataatttct tctgatgcat gatatatata atctttggca
     4561 tcctcacttt ctggtttaag ttcaaactct acaaataaaa ttgaaggtgt aaactcagtt
     4621 gtaggcattg gagatactat ctcgtaaaac atttcactct ttggaaattt catcataact
     4681 ctatcttctt ttctaatgac taaatcatca gtattattgt catccataaa catatataaa
     4741 ggagtgtcta aaacttcagc gatttttctc aatgaagaca aggatggttc aatagcatct
     4801 ctttctattt gagagatata acttatagat aaatctgtca atttggaaac atcacttaat
     4861 gtttttttct tgctttttct tatatttctc atcttttcac ctagcataag aacctccaaa
     4921 taataaaatt aatataaaat attcgcttaa aacaaagtaa ttatagcaca acatagaagt
     4981 agtggcaata agaatacata gtagattttg acaattaaaa tttaattaaa tccaaataaa
     5041 agtctatgaa aatgaattat tgttaaatta atacaaagaa aataataata aaaaaattta
     5101 atagaagagt attaattgaa ttaagattat cacattatat tttggaaaag agtaaatttt
     5161 cagtaaatta tcttgacata tatattgaag taataatata atttaaagta taaaatttta
     5221 aaaattgaat tataacttca aaaaataagg agggtaatat gaagataaca gatatcaaat
     5281 ttgaaaagct tagaattaaa ttaaaaaaac ctgtagtagt ttcatttgga gtaattgaat
     5341 atggagaaag tataatttta aaaattgaaa ctgatgaagg gtattgtgga tttggcgaag
     5401 cagcgcctct tgcagcagtt acaggtgaag ttttggataa tgttctttca gtattgctta
     5461 tgtttaaaaa agagttaata ggcaaagacc cactagatat tgaaaccata catacaatca
     5521 tggatggaat tattataggt aatacttctg caaaagcagc tattgacata gctttatatg
     5581 atattaaagg aaaaattatg aatgcacccc tttataaagt tcttggagga tttgatagta
     5641 aagtccaaac ggatattact ataagtatag ataagccaga aaaaatggca gtagaagctt
     5701 tagaaagagt aaaagaaggt tttaggatat tgaagcttaa agcaggtata aatcctgaag
     5761 acgatattga agctgtaaag ttaattagag aagccgttgg agagagtata agaattagaa
     5821 ttgatgcaaa tcaaggatgg aacgtaaata gcagtataaa tactatcaaa aagttagaag
     5881 aatttaatgt tgatgcaatt gaacaagcac ttccacattg ggatttagat ggtactgctt
     5941 acataagaaa taaaagtaat acaaaaatta tgatagatga atcattacat tcaccaatag
     6001 atgcaattaa ggctataaaa aagaatgcag ttgatacatt taatattaag ctgatgaaat
     6061 caagtggaat atatcctgct ataaaaatca acaacatagc tgaggcaagt ggagtaaatt
     6121 gtatgttagg atgtatgctg gaaacgagaa ttggtataac agcagcagca aatttaatag
     6181 catctaaaaa gaacataaca gaagctgacc ttgatagttt tatgttttgt gaagaattag
     6241 aaggtatatc tggaggattt gtaatggatg gagatattat gaatcttgtc aataaaccag
     6301 gtttaggaat agaagtaaac ttatgataaa tttggaggtt aaatatgaat acactttttt
     6361 caaatccagg tagtatactt gctgtaatga catcaatgat tgcattgggt ttttacttac
     6421 aaagatacaa agccattaaa agcttaggac cagcacttac aataatcata atgggaataa
     6481 tactttcaaa cttaaaagta gttccagtaa gtactgagtt atatggaact atttcaacat
     6541 atgctattcc agtttctatg actataatgt tgatgagtgt tgacttaaaa gaaatgacaa
     6601 aattatcaag agaaccttta attgcaattt ttgtagctgt attaactgta agtatcatgg
     6661 catttttatt tggattagta tttgcagaaa aaatatcaga aggttggaag gttgctggaa
     6721 tgtttgtagg tacatatact ggtggaagtg ctaatcttac agctattgga acaggtctta
     6781 atgtaagtag acaaactctt gctgcagcaa atgcagcaga ctatgtaatt ggagtaccaa
     6841 cattgatatt tatgtttgct ctaccagcaa tattaaaaaa ttcaaagaag tttaaaaaac
     6901 tttggccata tcatgtagaa gaatcagaat tagaggattg tcaaaatgaa gaatttatgg
     6961 agtcaaaaga atggagtatt aaagatatag cttggatgtt ggctataggt tttgttgtta
     7021 cagaagttgc aacaatactt gctggatatt tcaattcgag ttttagtagt gcagcaagaa
     7081 tacttttagt tacaacaatt tcaattataa ttgctcaatt aaagccagta aagaaactaa
     7141 agggaaattt agatttagga ttatttgttg cattattctt tttatgtaca ataggttttt
     7201 cagtagatat aaaagaattt ttaggttcaa cttttactat aacactttac tgttttagca
     7261 taatttttgc ttcatttgtt ttccatttag gcataactag acttttaaag ataaagtatc
     7321 aatatgtaat actttctata gtaggagcaa ttgctgatgg tcctacttca gctttagtag
     7381 ctgcttctgc aaagtggaat tcgcttgtaa gtgtagcagt cgtaatggga gtaattggtg
     7441 gagtattagg aaattatgca ggtatatcag tggcatatgc aataaagatg tctctaggat
     7501 tatagggggg agtttaattg ataaaattta aattatacat atctggacta tacagtggaa
     7561 atgtaatctt tgatggagat ttattaattg aaaagttgaa tccttttaca aataaaatag
     7621 aaagtttaaa accaataagc aaggaagaaa atacatacta tttaaatctt acaaaaatag
     7681 atttaaagtc tttatttaat aattttgatg tatatactaa aagcttagta aacacagata
     7741 aagacacagt tataaataac ttaggagaaa cgcattctaa acttaatgaa tatatatggg
     7801 ttcaaagaaa caaaaagttc ccactagata taattattgt agataataaa atagtaggtt
     7861 ttatctgttt atccagagaa acttgtacta tattaattat ggatggatat gaagagtata
     7921 ctgtattaaa agaatgggaa aaaactcata aaaatgaaga gatatattct ataagatttg
     7981 gtggaaatta tatgattgat atgaaagatg gtataaaact ttctacagat gtatatctcc
     8041 ctgattttgt agattctacc aaaaaagcac cgacaattct tatgagaact ccatatggaa
     8101 aagaaaatga taaagaaata tattataaat atgtacaaag aggatatgct gttgtaatac
     8161 aagatgttag gggaagaaat gaatcagaag gtaagtggga acctatgatt catgagaggg
     8221 aagatggaga tagtaccata aattggatag tatcacagga atggtcaagt ggaatagttg
     8281 gaatgcttgg agcatcatat cttggttatg tccaatgggc agctgcatct tcaggcaata
     8341 agcatcttaa agctttagtt agtatagtaa catcaggaag cccttttatt gatataccaa
     8401 gaaaaggagg agcttttgta tctggtatgc ttgcatgggc atttatggta tcaagaaata
     8461 aagttgatag aagcaaaatg gtaagagatg attgggatga tgttttaaac atacgaccaa
     8521 tagaaaatat acctgttgaa gctcttggat atagaattga atttctggaa gaatggttaa
     8581 agagagtaga aaaagatgag tattgggatt tgatggattg gcatttacaa aaagataaaa
     8641 taaatgtacc tgcattggtt gtatctgggt ggtatgatga taatagtatg ggaactacag
     8701 aggccttaga tgttataaag gactatgaaa aaggaaagag aaaggctatt ctaggtccat
     8761 ggatgcataa ttcaaatact ttaagagata taaatggtat ttctttagga aatagttcat
     8821 taagatatga tttggattat aattatctat tatggtttga taaatactta aaaggtatag
     8881 aaaataacat agatacgact gctccagtag agtattactc tgtaggttct aataagtgga
     8941 agacagaaga aaattggcca ataataaata agatagataa aagtatgtat ctaataagtg
     9001 atggaaatgc taacacatct ttaggaaatg gtagattagt atttgataat ggtttagaag
     9061 aaaagtatga tagttatata tataatccaa aagacccatc agtacaacta attgatatgt
     9121 cagagaatga agttggagtt ccaaataact acaaagattt agaaaaaaga agtgatatgt
     9181 tgtgttatac atcagatgct tttagtgaag aatttactgt aacaggtgat ataaagttag
     9241 agttttttgc atcaagttct gccaaagata ctgactgggt tataaaaatt atggacgtgg
     9301 atttagatgg aaattcaatt aaattagctg atggtatact tagtgctaga tttagaaata
     9361 gtttttataa gtcagagttt atggaagaag gagagattta taagtttacg attataactt
     9421 caaaaatatc aaacacgttt aaagttggtc ataaaattag actagatata acgtctagtg
     9481 ctaaaaactt tattttccaa aatagcaaca cgacagaggg atataatagt attgaatata
     9541 tagaagcaaa gaatacgatt tatcatggag gtaaatatcc atctaaattg atattgccaa
     9601 tagagaataa gtaaatttat atagattaaa tagtgaggag gtgtctcaaa atgaattttt
     9661 tagttcgtga gacactttct tttatacaaa atcaaatgta ttttcatcat ttcaacaatg
     9721 aaaaagaggc ttatctctct gttttgagat agcctcctat aagctaaatt gattattttg
     9781 tagctgcaac aacgctcatt tcaactaaca aatgctctct agcaagtcta gcttcaacac
     9841 aagctctagc aggttcaaat cctttttcaa cccaagcatc ccaaacagag ttcatagctt
     9901 caaagtcttt catatctctt aaataaatag taacagatag caaatgttgc ttatctgaac
     9961 catatttgtt taataaatct tctattttag ctaaaacttc agttgtttgt tcaattactc
    10021 ctccttcagc atgagtttgt ccacataaat aaactgtatt gttatgaact acagctctac
    10081 tcattcttcc agttccttca tatctcttta tttccatatt ttaaatacct ccatagttaa
    10141 aaaatctatt aaaaatctaa ataataaatt aaaaaaagtc tatactatat aaaattatat
    10201 tatatattct gataataatc cataaaaaca tataactttt tagctataat tattattgtt
    10261 attaattgaa aagatttatt agttacttga catagtttac acttttaatt atagtataaa
    10321 ttggaatatt tagataatta ttatttaatg tataagctaa gtttttaatt aatatggttt
    10381 tatacattta tatagaaaag aggtacgtat gagtagcaca atcaaatcaa atcgaataaa
    10441 aggtataatg tttataatag catcagcgct tggctttgca atgatgtctg cattcgtaaa
    10501 gctagctgga gatttaccaa gttttcaaaa agtgttcttt agaaatctgg tatcagctat
    10561 aatagctttt tggcttatca taaaacataa aggaagtcta actggtaaaa aagaaaattt
    10621 aaaagtactt ttatataggt caatatttgg aactctagga gttatattta actactatgc
    10681 aattgacaag ttgatgcttt cagatgctaa tatgctaaat aaaatgagtc ccttccttgt
    10741 agtaatattt tgtgcaattg ctctaaaaga aaaaataaat gtaaagcaga taggagctat
    10801 tataattgca tttataggag ctttattcat aataaaacca acatttagtg tagaaataat
    10861 tccatatctt gggggaattg caggagctgt atttgcagca atggcatata cttgtttaag
    10921 agttcttgga gatagagaag attactatac aattgtattt ttcttttcag ttttttcatt
    10981 agttactgta gggccaattg cttttgcagt atatgaacca atgaccctta tgcaacttat
    11041 atatctatta ctagggggag tatttgcaag tctaggtcaa tttggaataa ctctagcata
    11101 taaatatgca cctgctaagg aaatctctat atttgactat agtaatataa tcttttcagc
    11161 aattttaagt atattcttat tcaatgtcta tccagatatg ttaagtgttg ttggatattt
    11221 aatagtattt tcggcagcat tttatatgtt tttatacaat aagaagttag ataaattaga
    11281 caaagaaaag aagaagtaaa ctagataaaa gtgtttttat aatttgtatt gaatatttaa
    11341 gtctttgtat taaaattaaa gaaaatttaa ttaaaaatgt ttggtatatt aacttataac
    11401 agcagttaaa attgaaaaat tataactgct gtttctttta ttaaaaatat ccaaatgtct
    11461 gtaaaaatag actataatag agtagtataa ataatataaa ggggataaat taaagtatgg
    11521 aaaaagtgaa agttgctgtg attggagcag gtaatagagg aacgtatgca tatgctccat
    11581 atatttatga aaattctgat gtatgtgaaa tagttgctgt agcagaacct aaaaaaggaa
    11641 gacgagaact atttacacaa aaatataacc tagatagtaa aaatgtattt gaaactttag
    11701 aagacttttt taaacatgac aagatggcag atgcagtaat aatagctaca aatgatgata
    11761 gacattatga tgtagcaaaa ctagctcttg aaaaaggtta tcatgtacta ttagaaaagc
    11821 caatgtctaa tagtttagat ggtctggtac atatagatga cttgtgtgat aagtataaag
    11881 ataagatttt tatgatttgc catgtactga gatattctcc attttataat aaattaaaag
    11941 aaattgtaga aagcaaaaaa ttaggagagc tagttagtat acaatataat gaaaatatag
    12001 gatattggca ttttgcacat agctttacta gagggaattg gagaaatagc aatgaaacca
    12061 gccctttaat tttagcaaag agttgtcatg acatggatat tttgttatat ttagtaggaa
    12121 gtagatgcaa aaaaatatct gcatttggaa gcttaaagca tcttaataac caaaatgcta
    12181 gtggagagat ggcacaaaat tgtcttcagt gtttagttga aaaaaaatgt ccatattcag
    12241 caaagagaat ttatttagaa aaagatagaa gtataaatag agctgttcac ataaatccaa
    12301 ctgaagagaa tctattaaat atacttaaga caagtccata tggaagatgt gtttacagat
    12361 gtgataataa tgttgtagac aatatggtaa atatactaca gtttgaaaat ggagtgacgg
    12421 ctacttttaa tttatgtgca tttactaaag aaaatggaag aactataaag ttaatgttta
    12481 gccatggtga agttggtggg gatttaaata aaaatgagat aagaattaaa gaatttggaa
    12541 aaaacgaaga aatagttatg aatccatcaa atcaacagaa tatggtagag gaatatgata
    12601 gaaatcttat agctgaattt attaaattag tttcaaataa agaactagaa aagggtagag
    12661 tagcagcaaa agaagctata caaagtcatg taatggcatt tgcagcagaa tattctaggg
    12721 tcagtgatga agtagtgtat atagaagaat tttttgatag tgctaagcaa atgactaagg
    12781 agatagaaga aactatattt taattaataa aaatttatta aataaaataa agatagatta
    12841 aataatagaa atacgaaaat atgtgttcta ttaaggaatc tatctttttt attaaggttt
    12901 tttcttatcc ctatcaaaaa aatccataca ttctgataaa agataagaaa gtaaagcgaa
    12961 taagatttct gtacttaaag gaatcgttac tgaatccata aaggtatcaa ttatgttcat
    13021 agctagaaaa gttagtataa aatcaattat aaattcgcaa agtttatata ttgagtatgg
    13081 taatttgttt acatatctga atatatctaa aatagtagtg catagaaaat caattggagt
    13141 ggttatacaa aaatatatta aaaagaaaat taatacagct ccaagagatt tatattcaaa
    13201 tcctaagaat gataagaagc caattcctat aaccgtgaaa atacaaaaag ttataccaaa
    13261 aaaaacagca aaaaataaaa atacagaaat aaattcgcct aaaggaatcc ttcttttatt
    13321 acttatctta atcaactcct taatttatga tatgaaattt atataaataa taacattctt
    13381 aaaaattcat aatagtcctc tgtttcaaat agtactgttt gagaatctat ttttttcata
    13441 tttggataaa aagaggcctt aaaagcttta taatgttctc taaattttat ttctatctta
    13501 aatttatctg gcaattttat taagtgccta gataaatctc cagataaagc ttttttgaca
    13561 cctttttcaa ttttttttag tgcaaggttt ggatggattg aaattgaacc atttccaatt
    13621 ccttttgaca caggaacagt gactatattt ggatttagtt tttttgcact ctcacaaagc
    13681 atttcatctc cacttaaaaa tgctacagga acattataat atgcagcaat ataagcattt
    13741 atagtaaatt cagatgctat ttcatcattt attttaaaat agtcataatc aatatccata
    13801 gtatgggaaa gtggattacc attacaagta gctccagagt gatagccggt gaatatacat
    13861 gcatcaaagc tttcatcaat tccagccatc ataactagtg ggtctttggt ccatcctcta
    13921 agtattttga cgttttcagg taaaatattt ggattaatat ttctaccact gtcatgtgca
    13981 tctttaacaa gtatttcatg tacaccagat tgagtagctc catcacatgc tgctgttatc
    14041 tctttactca tttgtgttct atagtgttgc gaaaattcac attcaagttt tgtttcatcc
    14101 caatttacaa taccacaagt accttctata tcagcactta aatacaattt cataatatat
    14161 acccccttat gtttattatt gatgataata acagataaca gaaaatacat aatcaataaa
    14221 gggcataaaa atattatgtc ttactattaa ttattattaa atctgtaact gaaaaataac
    14281 taaattggta ttaaaaaata taacactatg tgtcatttat ttatatagtt tttatatctg
    14341 atgtttatat aaaattgtaa catataagtg gttacattta ctagtttaac atagttaaaa
    14401 tagttaggta ttatttttaa gttaaattga ttattataaa atcgcagaat aaaagaaaga
    14461 tttaatttat taaaatgcat gaaatgtttt ttcctcaaca taatgctaaa ttaaactcaa
    14521 aaagaaataa tagaaaaatc ttatgtagct tagaaagaat gtgaatgatt taagggaaaa
    14581 atagaaattt attatataat attaggctgt attatataat acagcctaat attatatgta
    14641 tatgtgacat aaaatcttgg taagtgtaaa taaaataaat gtggaaaaca agttaatgaa
    14701 atcaagaaaa aagtaattta aaattcaatt tcatcttccc aattaaattc tcctgatttt
    14761 tctacactct taggccagtg actacaccat ttaggaatgg acggattttc aaccctatca
    14821 acgcttggag tatatggctt taagtctata acaggactat tatcattagc atcaatatat
    14881 ggtattttta ttataccatt ttcatggtcg atattgataa tttgtacagc agtcaatgct
    14941 attggattgg gtcttatagg tgaccttgtt gcaaatattc ctataacgtc tggtccactt
    15001 ttataaggtt ttggggtttc taaaatactt cttagttcag gattatcaaa cccatcagcc
    15061 caccaaaata catttaaatg actgaaatcg tcgagttcat ttaatgcagg tacatacttt
    15121 ttgtctaatt taataaagac ctcttcgcct tgaatacata ttttacctat aggatttatt
    15181 aatatttctt tcataatatt acctccaatt atttaattta tcatttgtaa ttttagggta
    15241 taacatgaca tcatgtgagg gtcaatataa aataataaat aaaaatatta atagttagaa
    15301 tgataataca ttttttacat ggagcatgac accagtaata aggcaatctt cgtcaatatc
    15361 aaaagtagag gtatgcaaag gggaagtaat gtttttttca cgatttgcac aaccaaggtg
    15421 gtaaaaagct ccttcacagt gtcttagaaa atacgaaaag tcttcagccc caagagtggg
    15481 cgagtcttta aggataatat tttcttctcc aaacaattct acagcatttt gtttgacgta
    15541 atctactaga tggtttgaat tgataagagc tggataacct tcctcaattt caacagaaat
    15601 acttcctcca aatgttttac aagtaaaatc gcaaatttct ctaattcgtt tttttgaaaa
    15661 ctctctagtt tcaggagtca aggttctcaa agtacccctt ataaccactt tatcacaaac
    15721 tatattttcc ttgattccac cttcaatctt tcctagagaa ataaccacag aatcagttgg
    15781 atttgtgttt cgactaacga ttgtctgtag agctgtaata atatgagcag atataacaat
    15841 agcatcaata ccttgatgtg gataagctcc atgacatttt gaaccatgta cagttaattg
    15901 aagtgtatca gtggaagcat ttagagtgtt gtattttaat tcaattagat ttttgttgat
    15961 attggaattt acatgaagtc caaatatata ttttacattt gggttttcaa gacatccatc
    16021 ttctatcata agttgagccc caccaatagt ttcctcggca ggttgaaaaa agaattttac
    16081 atttacattt aagtaatcct ttaatttaaa tagtatatta caagtaccca gtaaaatagc
    16141 agtgtgtgca tcatgaccac atgcatgcat tttgccaata ttaatagatt tataagaata
    16201 attaagttct tctgttatag gtaaggcatc tatatcagct cttatagcaa ctgtttcaaa
    16261 gtttggactc acgttaatgt atgcagttat ccctgtatga ttcttatatt caatataatt
    16321 tatacctatt tcatttaagt atttaataat ttttttctta gtttgaaatt cttctaatcc
    16381 aagttctgga gtcttatgta aatctcttct tatatttata acccaatcat ttatctcatg
    16441 acaaagagtt tttatattaa gcataaaaca catcctaact atattaaaaa tttatttttc
    16501 ttcattaatt atattatttt atgtaaatca ttattatgta tcaagtacaa ataaaatatc
    16561 ttataattat attagagata tttagtatgg agaagaatat atcattttat aagaagaggt
    16621 gtttaaatgt taggaataat tgcgccatct gggccattaa gaaatacaag tttggaggaa
    16681 ataaagttta atttagaaag ttatggttat gaagttaagt tttctgaaag ttgtagttta
    16741 aattataaag gttatttagc tggtaatgat gatattagag caagggatat agaagatatg
    16801 tttttagata aagatgtaga tataattatg tgcttaagag gaggttatgg aacaacaaga
    16861 atattggata aaataaatta tgatataata aagcaaaatc caaaaccatt tataggtttt
    16921 tctgatataa caggtttaaa tttagctttc tataaaaatt gtggtctttt accatatcat
    16981 ggaataatgg cagcaaatgt aggtaaatgg gataaattta cctataaatc attggtaaat
    17041 gctttagaat ttaaagatga attatattta gaaaatccaa aagaagaaaa gatttatact
    17101 gtatgtgaag gtaaagcaga gggaataata atgggaggaa atttatcctt aataattgcc
    17161 acaatgggaa caaaatatga aatagatgca aaagataaaa ttttgtttat tgaggaaatt
    17221 ggagaacctc aatataaact ggatagaatg ttaacacaac tatattcttc aggaaaactt
    17281 gaggaatgta atggtataat ttttggagat tttaaggatt gtattgaaga aaatgatttg
    17341 atggaattac taatagaatt tgcaaataag gtcaataaac cttctatata taatcttcag
    17401 tcaggacatt gtatcccaat gataactatt cctttgggca gaatgtgcga attagatgct
    17461 acagaaaaaa tcgttaaatt aaaaaaatag tgtgtatata aatataatag aaatatggta
    17521 taatcttata caagtaaaaa tttagctagg aggatgaaaa atgtctgatg atagaataat
    17581 agactttaac gaactaaaga acaaagttaa agattctgat gtagataaat ttgagcaata
    17641 catatataac ttatattttt ctgttatgga tggaaagatg tctatggctg agttttcaag
    17701 aaaaatattc gattatatga gagataacaa catatctcaa gaaaaattta tgaaaataca
    17761 aaagcaattt atggagagat atggtatgga cactgaagaa gtagaaaaac aacttagaaa
    17821 ctttggaatc gacccttcta cggcaggttt tatgagtaat aatacttcca gcaaagtatc
    17881 tacagaagat ttagagtcat ttaagaagag tgcaggattt tatgagaaat atggagaaaa
    17941 aatccaacca aagagttgta taacaacatt tataaaaaat gacttaaatg atattaatgt
    18001 aataatcgac caagaaaaga taatgctttg tagtgataga aaaataaatc ttatggattc
    18061 agaactaaat gagtttttat tagaatataa gaatatgttt aataaaaaaa taaaggtagt
    18121 aatgtgcgaa actacgaata agtatgatta ttaaaaaatg aatttaaata ctgaagtagt
    18181 atcaattaat tgtactactt aaaaataaaa atcgcaattt attattcctt ataaatttgt
    18241 ataaggaata tctaaactgc gattttttat attgtgaaga ttagtaataa tagatagtgt
    18301 tttaaattgt aatcttctca ctaaaactgt gtttttctat tagaaatttt attaaaaata
    18361 aatatctgat aattgtatta tatgggatgt aaagtattag aagcttaacg aatctctttt
    18421 aatacattaa ctccattttg aatcatactg ttgaaaaata ctaagtttaa aatctcagca
    18481 gggtgaacgt taggaatatc ataagtatct actaaattca taggtacaac tatatttttt
    18541 tcaatattat gttgattaaa atatgatttt agagttattg caaattgata tatacatata
    18601 tctgtgcaat cgccaactat aattatatta tctatattat caaaatccaa attttctaat
    18661 gcaaaaaaac cattagtaga atttttagga agaatttgta ggttttctat cgacttaagt
    18721 tcatcaacca attcactttc tacatcattt tccaaacagt gaacaggata actcaaaagt
    18781 tctattgatt taggagtatg agaatcagta aatgctatta ctctatttaa tttgtttgat
    18841 attttttttg taaacatttc aattggcttt attaaagact ctactctagg agagtataat
    18901 gcaccttgtc ttgcaaagcc attattaaca tcaattataa ataaagcagt tttactcaaa
    18961 tcataatttt ctattttttc tattggtaga ttatctaaat tactctttaa agcttcaagt
    19021 tcatttaata agttatccat agttatacct ccaagataaa tttaataaaa ttgtacatat
    19081 taattatata aaagtcaagg agtacaaact attgtataat tagctgtatt gttttgagtg
    19141 taaaagattt gaagcaatat ctggatagtt agtaaaaata ccatctacat tgagttttag
    19201 acagtgtagt atatcatcct cttcatttac agtatataca ttgacaccaa gtagattttt
    19261 gtgacaaagt tcaattagag attcatttac taaaactaga ggtggatgaa gataactatt
    19321 acccagaaat ttagcaaaat tgactacatt gtttataggt aaataacaca ataatcctgt
    19381 ttttacacta gggtctaatt tatgaaagtt ttttaagcta ttgctgttga aactagatat
    19441 aagaactcta tttttcatat tatactttaa aataagctct aaaacgcctt tttctatatt
    19501 gggatagttt attttgtcag tttttagttc gatatttaaa gtaaggtctg taggtgagat
    19561 tagaattaat acttcttcta aagttggaat tttacattct ttattgcttt gatatccttc
    19621 aaaagatgag tttagagttt ttaattttct taaagtaaag tcttttacaa aaccatgtcc
    19681 attaaaagtt ctatcaacct tttcatcatg aataactaca atttttccat ccttagttct
    19741 gtggacatca agttctattc catatatatc catatccaaa cattttttaa aagctagaat
    19801 agtattttct ggatactttc cactaaaccc cctatgagca taaatattca tttatatggt
    19861 tccctcctat aagtataact tatcttataa aa
//
LOCUS       DAMJYR010000062        19286 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.062, whole genome shotgun sequence.
ACCESSION   DAMJYR010000062 DAMJYR010000000
VERSION     DAMJYR010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 19286)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19286)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19286
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.062"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            335..913
                     /locus_tag="PC471_002240"
     CDS             335..913
                     /locus_tag="PC471_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_126936537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HDF2789368.1"
                     /translation="MGITLSKGQKVDLTKGNPGLKNILVGLGWDTNKYDGGFDFDLDT
                     AAFLTGASGSVTNDGDFVFYNNLKHTSGAVEHLGDNRTGEGDGDDEQIVVDLSKIPGE
                     ISKISFTVTIHDATERRQNFGQVSNSYIRIVDKDTNEELIKYELGEDFSIETAIVVAE
                     IYKHNGEWKFNALGSGFEGGLAALCGNFGINL"
     gene            952..1530
                     /locus_tag="PC471_002241"
     CDS             952..1530
                     /locus_tag="PC471_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_126936537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HDF2789369.1"
                     /translation="MAIVLKKGQKIDLTKGNPGLKNIKLGLGWDTNSFDSGYDYDLDV
                     SVFMVGESQRVEKDEDFIFYNNLKHPSGAVEHLGDNRTGEGDGDDEEILVNLETMPKH
                     IQKIAVTVTIYEAAERRQNFGQVSNSYIRVLNSENDEEILRYDLGEEFSIETAITVCE
                     IYKYNGEWKFSAVGAGFEGGLEALCRNYGLNV"
     gene            1616..2245
                     /locus_tag="PC471_002242"
     CDS             1616..2245
                     /locus_tag="PC471_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HDF2789370.1"
                     /translation="MSINLSKGDKIDLKKSNPGLSNILVGLGWDPVQQSGGGFFKSLF
                     GGGQADIDCDASVFMLNQEGKLSGIKDLIYFGNLKSACKSVLHTGDNLTGEGTGDDEQ
                     ILVNLDKVPSNIHKLLFVVNIYNCVDRKQHFGMIENAYIRVEDQGNKKEIAKYNLSDN
                     YSEKTTLIVGAIYRKDGSWQFKAIGEGTKDAGLKEVMQNLDRIECAYGI"
     gene            2235..4994
                     /locus_tag="PC471_002243"
     CDS             2235..4994
                     /locus_tag="PC471_002243"
                     /EC_number="7.2.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-translocating P-type ATPase, PMCA-type"
                     /protein_id="HDF2789371.1"
                     /translation="MEFNNDIKYYNVDKEEVTKDLSVNPEKGLSESEVKTRREKYGLN
                     EFTPKEEGSFWDDLKESLSEPMIVILIIAAVVSAVIGETHDAIGIVGAIAIGIAIGMI
                     TEGRSKKAAEALSKLTENIEVKVLRDGEVHQISKSELVPGDIVYIETGDMVPADGRLI
                     ESINLKIREDMLTGESDDVSKKCDVVVSMEEIESKGAVVVQEPIPAKQINMVFGGTLV
                     AYGRGALVVTSIGDNSEMGKIAQNLSETDEQTPLQIKLGNLGGLIAKVSSAVAGLLFI
                     FMVFQMISKNVLNVDMSGILPFLESIDPVKTAFTVCVALIVAAVPEGLPTMINMTLAI
                     TMQKMAKINALVTKKEACETIGSVSVICSDKTGTLTENRMTVEVAYVDGRYIESSEEE
                     INSYFEENCMINSTADVEHNDGDIKYLGSATECALLLYYKNVDYRQVRKNSNIVAQNP
                     FTSDSKRMTSVIGQDNHHVLLSKGAPEVLLELCSHIQRGKDIVPLTENIKHEILEEIK
                     KLQIKSMRTLGFAYKEISQAEEEAAVTAESDAMNVSAMENNLVFSGFVGIRDPLRKDV
                     IESVNTANKAGVSVKMLTGDNINTARAIGEELGLLKNNMRVVEASYIDTLDDEELKQE
                     IQSISIVARSKPDSKMRIVSALQKSGDVVAVTGDGINDAPALSKADVGIAMGISGTEV
                     SKNAADIILTDDSFSTIVKGIKWGRGIYDNFQRFVQFQLTVNVIAFLVAIISQVMGQE
                     MPFTTIQLLWVNIIMDGPPALALGLEPVRDHVLNRKPVDRKANIISKSMVYTIVLNAF
                     YITAILMLQSTFNFLGVDEAHKGTVMFALFAFSALFNAFNCREFNADSIFPNFTHNKL
                     ALQIIVLTGIAQVIFTQLFQDFFNSVAMDFMTWVKVLCVAASVIVVNEVVKCIIRLVK
                     LKKTA"
     gene            5081..6643
                     /locus_tag="PC471_002244"
     CDS             5081..6643
                     /locus_tag="PC471_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceG family protein"
                     /protein_id="HDF2789372.1"
                     /translation="MGDSKVFEKIFSSQSDRGNYTPSKGYLSYFISYIGLEDEVLYNL
                     EIFKTKQNIDSKKDIALFTDVIANPSDFDIINYFKSGLQKYRTSMEDVDINILGFEEI
                     DYKIKQAMDRVLKEEEKEFTNDRVKQNFIVKIMAWIKIYIGALDINKNEAPKVIFYGD
                     IKKHEVYLLLILYLAGFDVLYLNPNSKSNIDILKSERYNIEFEEANIIEEKISFEERV
                     ILGEKIDKSSVKKAFTVGAEASKRISEELLNDAGFIKPWQLQDRKIKNLLLSSTVDEI
                     SIYWNQPLKLRPGFKFNDAIVEAPNFLSKINGIYNDKNEYIKFLDLLRDSESSTFIEF
                     NGDVDRFSKAFTREAFSLSFLLDSKGAIDKNSVLNNKDYSISTLALNQQIMILEKVEE
                     LLEGSMFLNGLSGEDKIKGLFTVLHMDKKFVHMMNNFDYSLINPKLIIYMYKSIVFDK
                     EIVFLMLLLSKIGFDIIILCPGGENNIENVINNQLIDVHRLDKMVYDLKLNSLENDIP
                     LLKKIFGKRRRF"
     gene            6644..7780
                     /locus_tag="PC471_002245"
     CDS             6644..7780
                     /locus_tag="PC471_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HDF2789373.1"
                     /translation="MGININSFSKDKNSVEVTENLPTAVQEENFDIMEYTNNKKNELR
                     KSKEVEALTSLIEVENPDTILQFGRKASEGVARVSDSLLNTIKLNRNEENSKMLVHLT
                     KIMDKFDLDDFQETKEPNFVQKLFKKANNAIEMMFQKYETLGGEVEKIQIELEQYERD
                     IALSNKQIGAMLNENFEFYNELQKYIVAGEMAIEEMDNEILPFFKQKSETSGDQMDVV
                     NYQELLKVYDMLNQRVYDLRIAENIAIQTIPMLRGMQHNNYGLIRKINSAFVVTLPVF
                     KQCLSQAILLKKQELQAKSLKALDDKTNELLLRNAQNVSTQSAQIARMAGTSSVQIET
                     LEKMYNTIKSGIDETMRIEENNRALIKDNTKRLEELNTTIIYNK"
     gene            7844..8920
                     /locus_tag="PC471_002246"
     CDS             7844..8920
                     /locus_tag="PC471_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HpcH/HpaI aldolase/citrate lyase family protein"
                     /protein_id="HDF2789374.1"
                     /translation="MKYFDYICKDDLERIFLKEPEDFSAKTEKDVLKYALGAFLYVPA
                     TQYNMIYKSIIGDVKGVRPLAICLEDAVGVNGELEAIENLRLILKNISNESITNKDGI
                     PLIFVRIKDVEQLLRIKEIIIKNSHFITGILIPKANSELIENCIEALDFMNLQDMYVI
                     PIVETKEFIYNEKKELSFTNLYNAILRHKSRILSIRVGITDILGMYGIRRDKNFSIYN
                     NLICSSFILDIITYLQRPELDIPISGGVSEFFDMTNKDIRNKYIEEILLDKYHGLIGK
                     TVIHPMQIQIVQALSTVSYEDFTDALDILDSTDSKYGVSKGVLGERMNETNPHFLWAK
                     KTLILSKIYGVLNKGVDYEELLKF"
     gene            8898..10136
                     /locus_tag="PC471_002247"
     CDS             8898..10136
                     /locus_tag="PC471_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HDF2789375.1"
                     /translation="MKNCLNFNCEIDIIDNPLNLELNDFLDVGIRNNSKRRFLFISKK
                     LGKHLACKPSEMDNLGKLMATIYNEKNKEESNGTVIAFAETGTAVGHSFFDYLCGDYE
                     FIHTTREQFDELENLEFLEEHSHATDQNLYFGMLNNFKFGDEIILVDDEITTANTCMN
                     IIRKIQSIYPKKKYTICSILNWVSDENLSKVTRLERELDCKIDFVYLFKGNFKFYIDE
                     DIISNYNDNYSITKLETISSDKVNLVVNHIYVDLEDYIGNKKYVKYTGRFGINRREQF
                     RLKEIVKRESKKITMASKESLKNEKEKILFLGTEEFMYIPMLFAKEKENQYDIYYHST
                     TRSPIINIDKNNYPIKSKFMLKSFYNIDIQNYIYNIDKHNFSKCYLFVELNKSENSYL
                     EFIDIIKKTSIKELTIVCCS"
     gene            10190..11278
                     /locus_tag="PC471_002248"
     CDS             10190..11278
                     /locus_tag="PC471_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine protease StiP family protein"
                     /protein_id="HDF2789376.1"
                     /translation="MRDFSTYSKDDVIFLLKDISNLIEEEGNKERELKIQNGRHYSEM
                     IPIEYEVSEDYLNLYHEKLRENKDKLAFSIGVMCEKIIKKNGNDVVLVSLARAGTPIG
                     ILAKRYIRSKYNISLPHYTISIIRDKGIDMNAIEYIVKNHPSSKIQFLDGWTGKGTIS
                     KELEKACDELNVKFKKYFDSTLAVLADPAGYSGLYGIREDFLIPSACLNSTVSGLVSR
                     TVLREDLIGKNDFHGAKFYRHLKDKDESMNYIETIEECFKNQFKNISDEVENWESDII
                     TRDGYFDVLNIKEKYNITDINFIKPGVGETTRVLLRRVPYKILVKDLNDKSLDHIFIL
                     AKEKNVEVEQMDLKAYKCCGIIKNMKDI"
     gene            11306..12097
                     /locus_tag="PC471_002249"
     CDS             11306..12097
                     /locus_tag="PC471_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HDF2789377.1"
                     /translation="MIVFSDLDRSIIYSNKFLNADSKYTNIEIYREKEISYISLDTIN
                     LIKQIQYYGMFIPTTTRTVEQFKRIEFNKYGIYFPWSITSNGGVILKDNEILKSWSEK
                     IDKLKCDYEPIESMIHKFKDYLNVDGITNFKVAEDTFFYIVVDLSRFNLDSIKEYTNI
                     LESKNWKFYVSGRKIYFIPKEISKENAIKYLTKELGIEYFYAVGDSIMDYGMLNISNK
                     SYVLKHGDINKNEIENSFISSSFNGMSGTEEILSNILDENCLLNI"
     gene            complement(12212..12766)
                     /locus_tag="PC471_002250"
     CDS             complement(12212..12766)
                     /locus_tag="PC471_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HDF2789378.1"
                     /translation="MNIDVGSKIRSLRKSKNISISTLAKNSDLSTGLISQIERNMVVP
                     SIVAMWKISKALDINIGYFFEEIGREDSDIVVKKNNRKKIVTNDSTKFYELLVPNLSG
                     KKIEFILVTLDGHTQKNSEFVTHEGEECGYIIKGKMKITLGNKEYLLEEGDSFYFNST
                     IPHVYENYDDEVCISVWAMTPPSF"
     gene            12962..14164
                     /locus_tag="PC471_002251"
     CDS             12962..14164
                     /locus_tag="PC471_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF819 domain-containing protein"
                     /protein_id="HDF2789379.1"
                     /translation="MKSFVSPDNIWVLWAVVTGWAAFSIYLEQKYNWASKVSGAIIAL
                     VGAMLLSNLNIIPVESVVYDQVWGYVVPLAIALLLYQCNIKKIWKESGRLLIIFLVGS
                     VGTVLGAMIGFLALKNVVPDLNIVAAMMTGSYIGGNVNFAAMSGAFDAPGELVSATVV
                     ADNLLMALYFFVLIAIPSIGFFRKHFKHPHVDEMESIGITEGETVAANYWGRKEISLK
                     DIAIAIGSAFIIVAVSVELSTWFKAIIPLSNPFLAMLNTLFGNQYLIITTLTMLFATF
                     MPNFFGDIKGAQELGTFLIYIFFVVVGVPASITLIIQKSPLLLLYCGIMVLINMLVTF
                     IVAKIFKFSLEEAILASNANIGGPTTAAAMAISKGWSKLVGPILIVGTFGYIVGNYFG
                     LLVGNILI"
     gene            14221..15201
                     /locus_tag="PC471_002252"
     CDS             14221..15201
                     /locus_tag="PC471_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane dipeptidase"
                     /protein_id="HDF2789380.1"
                     /translation="MIFDGHSDTWADVTIRRLNGEKDVFRRIHLDNFKRANIEGAIFV
                     IWIDPPYDKEPEKRVKQIISSIKDEIEDMKDVVNIVKKYSDIDKGISENKINILIGLE
                     GLSHIGRNIDLLDYFYKEVGARHASLTWNEQNELATGCLGDENRGLSEIGKKAVNKME
                     KLGMIVDVSHLNEKSFWDLMSVASKPVIASHSNARSLCDAKRNLTDNQLKEISNTGGL
                     VGINSFRGFTNSNPKKQDLIHLVDHIDYMVNLIGIEHVSFGFDFSDYLGKETIGSFTG
                     NASVPSVDGLESTHTAQNIVVELENRGYKKKDIELISSGNFYRLFKDILK"
     gene            15444..16397
                     /locus_tag="PC471_002253"
     CDS             15444..16397
                     /locus_tag="PC471_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HDF2789381.1"
                     /translation="MKKRYLVIGIIILSYFSLFIGAEDINIMHIFAKNQHKLMIFIMS
                     RIPRLISILIAGVGMSIAGLIMQQISKNKFVSPTTGATIDAAQFGIVICMLLVPTASI
                     FTKTIIAFVFSLVGTFTFMKIIGKLQFKNIIFVPLVGIMFGNIIGSMTDFIAYKYDLS
                     QNVSSWMQGDFSMILKGNYEILYITIPLIILAYIYANKFTVVGMGMDFATNLGLSYKR
                     IVNIGLIIVALVTVCVVVTAGNIPFIGLIVPNIVSLYMGDNIRASIWYTGLLGAIFVL
                     ICDIFGRIIIYPYEISIGLTVGVIGSILFLYLILRRNVNEA"
     gene            16387..17343
                     /locus_tag="PC471_002254"
     CDS             16387..17343
                     /locus_tag="PC471_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HDF2789382.1"
                     /translation="MKLSLNQKIYFVSILLFISMALFIIYGIDMNHLEYALSQRIPKI
                     FAMVLGGGCIAFTTVVFQTITNNQILTPSVLGLDSLYVMIQTIIVFIFGSSSSLIINE
                     NYNFIINVAMMIGASLLLYKTLFEKNKNNIFFLILVGMIFGTLFKSATTFIQVMIDPN
                     EFLALQTSIMASLNNINTNVLLIAFIIIIAIIPFIYDEIKYLDVLSLGKEQAINLGVD
                     FDKAVKKMIILIAILVSISTALIGPMTFLGLLLANITREIFKTYKHTYLISGSMLIGM
                     ITLIIGQFFIQHVFKFDTTLSVVINFIGGIYFIQLLLKGANR"
     gene            17340..18095
                     /locus_tag="PC471_002255"
     CDS             17340..18095
                     /locus_tag="PC471_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDF2789383.1"
                     /translation="MIEIKNIFKRYKNKNVVDDVSFSIEKGKITSFIGPNGAGKSTVL
                     SIVTRLIGGDGGEVIIEGKSLTNYSNKELAKKIAILKQSNNITLKLTIRELVGFGRFP
                     YSEGNLTKEDENYIDEAIEYMKLTDIQHKYLDELSGGQRQRAYIAMVIAQDTEYILLD
                     EPLNNLDMNHSVQMMKVLRGLCDELDKTIVLVMHDINFASCYSDNIVALKNGKVEKVG
                     RTDEIVNEKVLEDIYEMNFNIKNINGNRICIYF"
     gene            18187..19152
                     /locus_tag="PC471_002256"
     CDS             18187..19152
                     /locus_tag="PC471_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HDF2789384.1"
                     /translation="MNKKAAIVAAVAIIGLVTVFALGGSKKNESKTSENSNNTMKITH
                     NLGETDVKLNPKKVVVFDYSALDTMDALGVAETLVGLPKASLPASLEKYKDDKYADLG
                     GLKEPDLEGIKSANPDLIIINGRQEDFYEQLSKIAPTISTSKDDKKYLESVKNNIDKI
                     AKIFGVEEKANQEFSKIEKKIETLNKKVTDKNLNALTIMVNEGNLSVFGEESRFSILY
                     NSFGFENKDKNIKESSHGQNITFEYIAKQNPEVMFVIDRGIATGSDVKESSTAKSVLN
                     NDIIKSMDAYKNDNIIYLDSPTWYVNDGGLTSLNKMIDDASKAVN"
BASE COUNT         7622 a         1963 c         3453 g         6248 t
ORIGIN      
        1 ggtagtagtg gcgaagacac cgtccgaagt agcttggctt tgtaaccacc tctgttgtac
       61 tgtttaagta taaataagtg tgttagccac caagaatccc cttgctttag ctatggggag
      121 tgtgtcaaat attaaacata gcttttatac atttaataag gtatgtaaaa tctatgtaaa
      181 aagtaagaaa tataaacatt tttttaatta tattttaaaa atatcaagca agttttcttc
      241 acaaatgaat aaaaaatgtt ataatatgat taaagaaaaa gataaataat tagcaagatt
      301 tgtttagcta aaattaacaa gggagcgata aattatgggt ataacattat caaaaggcca
      361 aaaggttgat ttaactaaag gaaatccagg tcttaaaaat attttagtag gtttaggatg
      421 ggatactaac aagtatgatg gtggatttga ctttgattta gatacagcag catttttaac
      481 aggagcaagt ggaagtgtta ctaatgatgg agattttgta ttttacaaca atttaaaaca
      541 tacatctgga gcagttgagc atctaggaga taacagaact ggcgaaggtg atggagatga
      601 tgaacaaata gttgttgatt tatctaagat tcctggagaa ataagcaaga tttcttttac
      661 tgttacaatt catgatgcaa cagaaagaag acaaaatttt ggacaagtaa gtaattctta
      721 tataagaata gtagataaag acacaaatga agagttaata aagtatgaat taggagaaga
      781 ttttagtata gaaacagcta tagtagtagc tgaaatatat aagcacaatg gagaatggaa
      841 gtttaatgca ctaggttctg gctttgaagg tggtcttgct gcattatgtg gaaactttgg
      901 aataaactta taatttcaac tataacaata aactaaaggg ggatattttc catggctata
      961 gtattaaaaa aaggacaaaa aatcgattta actaagggaa atccaggtct taaaaatata
     1021 aaacttggac taggatggga tacaaattca tttgatagtg gctatgatta tgatttagat
     1081 gtaagtgttt tcatggttgg agaatctcaa agagttgaaa aagatgaaga ttttatattc
     1141 tacaataact taaaacatcc atctggagca gttgaacact taggtgataa tagaactggt
     1201 gaaggtgatg gagatgatga ggaaatatta gtgaatttag agacaatgcc aaagcatata
     1261 caaaagatag ctgtaacagt tacaatatat gaggctgctg aaagaagaca aaattttgga
     1321 caagtaagta actcatatat aagagtatta aattctgaaa atgatgaaga aatattaaga
     1381 tatgacttag gtgaagaatt tagtatagaa actgcaatta cagtatgtga gatatataag
     1441 tataatggag aatggaagtt tagtgcagtt ggagcaggat ttgaaggtgg cttagaagca
     1501 ctttgtagaa actatggctt aaatgtttaa tttaagcaat ataatatatt aaatataaat
     1561 aacctataca aataatgtat aggttatatt atataaaatt ttgtggaggt ttagtatgtc
     1621 gattaattta tcaaaaggtg ataaaataga tttaaaaaaa tctaacccag gactgagtaa
     1681 catacttgta ggtctaggat gggaccctgt gcaacaatct ggtggaggtt tttttaaatc
     1741 gttatttggt ggaggacaag ctgatataga ttgtgatgca tctgtattta tgcttaatca
     1801 agaaggtaaa ttaagtggaa taaaagattt aatttatttt ggaaatctaa aaagtgcctg
     1861 taaatcagtt ttgcatacag gagacaatct aactggtgag ggtacaggtg atgatgaaca
     1921 aatcctagtt aatttggata aagttccatc aaatattcat aaattattgt ttgtagtaaa
     1981 tatatacaat tgtgtagata gaaaacaaca ctttggaatg atagaaaatg cctatataag
     2041 agttgaagac caaggtaata agaaagagat agcaaaatac aatctatctg ataattattc
     2101 tgaaaaaact actcttatag taggagctat atatagaaaa gatggaagtt ggcaatttaa
     2161 agcaattggt gaaggaacaa aagatgcggg tcttaaagaa gtaatgcaaa atttagacag
     2221 gattgagtgt gcatatggaa tttaataatg atatcaaata ttataatgta gataaagaag
     2281 aagttacaaa agacttatct gttaatcctg aaaaaggttt atcagaatca gaagtcaaga
     2341 ctagaagaga aaaatatggt ttgaatgaat ttactccaaa agaagagggt agtttttggg
     2401 atgatttaaa ggaaagttta agtgaaccaa tgatagttat cttgattatt gcagcagtag
     2461 taagtgcagt aattggggaa actcatgatg caatagggat tgtaggtgct atagcaatag
     2521 gtatagcaat aggtatgatt acagaaggtc gttctaagaa agcagcagag gctctttcta
     2581 agttgactga aaacatagaa gtaaaagtat tgagagatgg ggaagtacat caaatttcta
     2641 agagtgaatt ggtaccaggt gatatagtat atattgaaac tggtgacatg gtacctgctg
     2701 atggtagact tatagaaagt ataaacctta aaattagaga agatatgtta acaggagaat
     2761 ctgatgacgt atctaaaaag tgtgatgtag tagtttctat ggaagagata gaatctaaag
     2821 gagcagtagt agtacaggag ccaatacctg caaaacagat aaatatggtg tttggtggca
     2881 cacttgtagc ttatggtaga ggagctctag ttgtaacttc aattggagat aatagtgaaa
     2941 tgggtaaaat tgctcaaaac ttatcagaaa cagatgagca aactccactt cagataaaac
     3001 ttggaaattt aggtggtttg atagccaaag tttctagtgc agtagcagga ttactattta
     3061 tatttatggt tttccaaatg ataagtaaga atgtattaaa tgtagatatg tctggaatat
     3121 taccgttttt agaatcaata gacccagtta aaacagcatt tacagtatgt gttgcgttga
     3181 tagttgcagc agtgccagaa ggtcttccaa ctatgataaa tatgacactt gcaataacaa
     3241 tgcaaaaaat ggctaaaata aatgcattgg ttactaaaaa agaagcttgt gaaacgatag
     3301 gttctgtttc tgtaatatgt tcagataaaa ctggtacatt aactgaaaat agaatgactg
     3361 tagaagttgc atatgttgat ggcagatata ttgaaagttc agaagaggaa ataaatagtt
     3421 attttgaaga aaactgtatg ataaactcta cagctgatgt agagcacaat gatggtgata
     3481 ttaagtattt aggaagtgct actgaatgtg cattgctttt atattacaaa aacgtagatt
     3541 atagacaagt tagaaaaaat tctaatatag tagctcaaaa tccttttact tcagattcaa
     3601 agagaatgac ttctgttata ggtcaagata atcatcatgt actattatca aaaggagcac
     3661 cagaagtctt acttgaatta tgttcacata ttcaaagagg aaaagacata gttccattaa
     3721 ctgaaaatat aaaacatgag attttagaag aaataaagaa attgcaaata aaatcaatga
     3781 gaactttagg gtttgcatat aaagaaatct cacaagctga agaagaagca gcagtaactg
     3841 ctgaaagtga tgctatgaat gtaagtgcaa tggaaaacaa tttagtattt agtggttttg
     3901 ttggtataag agacccactt agaaaagatg ttatagagtc tgtaaataca gcaaataaag
     3961 ctggtgtttc tgtaaaaatg cttacaggag ataatataaa tacggcaaga gctataggtg
     4021 aagagttagg cctattaaaa aataatatga gagtggttga agcttcatat atagatactt
     4081 tagatgatga agaattaaaa caagagatac aaagtatttc aatcgtggca agaagtaaac
     4141 cagatagtaa aatgagaata gtttcagctc tgcaaaagag cggagatgtt gttgcagtaa
     4201 caggtgatgg tataaatgat gctccagcac tttcaaaagc agatgttggt atagcaatgg
     4261 gtatatctgg tacagaggta tctaaaaacg cagcagacat aattttaact gatgatagtt
     4321 ttagtaccat tgtaaaaggt attaaatggg gtagaggaat ttatgataac ttccaaagat
     4381 tcgttcaatt ccaattaact gtaaatgtaa ttgcattttt agtggctata atatctcaag
     4441 ttatgggaca agagatgcca tttactacaa tacaattatt atgggtaaac atcattatgg
     4501 atggaccacc agcactagca ttaggtttag agccagttag ggaccatgtg ttaaatagaa
     4561 aacctgttga tagaaaagcc aatataattt ctaagtcaat ggtgtataca atagtattaa
     4621 atgcatttta tataacagca atactaatgt tacaatctac atttaatttc ttaggagttg
     4681 atgaagcgca taagggaaca gtaatgtttg cgttatttgc ttttagtgca ctgtttaatg
     4741 catttaactg tagagagttt aatgcagata gtatcttccc taattttaca cataacaaat
     4801 tagcactaca aataatagtg ttgacaggaa ttgcacaagt aatatttact cagctattcc
     4861 aagatttctt taactctgta gcaatggatt ttatgacatg ggtaaaagtt ttatgtgtag
     4921 cagcaagtgt aattgtggtt aatgaagttg taaaatgtat aataagactg gtaaaactaa
     4981 aaaagactgc ttaaaacaaa ttttttagaa gaaaacagac tgactcaaaa tatgaatttt
     5041 gggtcagttc tgaataaaca tttaaattgg gaggatagtt atgggtgact caaaggtatt
     5101 tgaaaaaata ttctccagcc aatcagatag aggaaattac actccttcaa agggatatct
     5161 aagttatttt atttcgtata ttggtttaga agatgaagtt ttgtacaatc tcgagatatt
     5221 taagacaaaa caaaatattg atagcaaaaa agatattgct ttatttactg atgttatagc
     5281 aaatcctagt gattttgata ttataaatta ttttaaaagt ggacttcaaa aatatagaac
     5341 tagcatggaa gatgtagata taaatatttt gggatttgaa gagattgatt ataaaataaa
     5401 gcaagcaatg gatagagtgt taaaagaaga ggaaaaagaa tttactaatg atagagttaa
     5461 acaaaatttt atagtgaaaa taatggcatg gataaaaatt tatatcggtg ctttagatat
     5521 aaataaaaat gaagctccaa aagtaatatt ttatggagat ataaaaaaac atgaagttta
     5581 tttactctta atattatact tggcaggttt tgatgtttta tatcttaatc caaattcaaa
     5641 gagtaatata gatattttaa aatcagaaag atacaatata gagtttgaag aagccaacat
     5701 aatagaagaa aaaatttctt ttgaagaaag ggttatttta ggggaaaaaa tagataaaag
     5761 ttctgtaaaa aaagctttta ctgttggggc agaagcatca aagcgtataa gtgaggaatt
     5821 gttaaatgat gcaggattta taaaaccttg gcaactccaa gatagaaaaa taaaaaatct
     5881 acttctaagt tcaactgttg atgaaatatc aatatactgg aatcaaccac ttaaattaag
     5941 accaggattt aagtttaatg atgctattgt agaagctcca aattttcttt ctaagattaa
     6001 tggaatatat aacgataaga atgaatatat taagttttta gatttattaa gagattcgga
     6061 aagttcaaca tttattgaat ttaatggaga tgtagataga ttttcaaaag cttttacaag
     6121 agaagctttt agtttatcct ttttattgga ttcaaaaggt gcaatagata aaaactctgt
     6181 tttaaataat aaagattatt ccatatcaac actagcattg aatcaacaaa ttatgatttt
     6241 agagaaagtg gaagagttgt tagaaggaag tatgtttttg aatggtttaa gtggagaaga
     6301 caagataaaa ggtctattta cagtacttca catggataag aaatttgttc atatgatgaa
     6361 caatttcgat tactcactta taaacccaaa attgattata tacatgtaca agagtattgt
     6421 atttgataaa gaaatagttt ttcttatgct tttacttagt aagattggat ttgatattat
     6481 catattatgt ccaggaggag aaaacaatat agagaatgtt ataaataacc aattgattga
     6541 tgtacacaga ttggataaaa tggtatatga ccttaaatta aattcgcttg aaaatgatat
     6601 acctttactt aaaaaaattt ttggaaagag gagacgattt tagatgggga taaatataaa
     6661 tagtttttca aaagataaga actctgtaga ggttactgaa aatttgccta cagcagttca
     6721 agaagaaaac ttcgatataa tggaatatac aaataataaa aaaaatgaac tgagaaagtc
     6781 taaggaagtt gaggcattga catctcttat agaagtagaa aatccagata cgattttaca
     6841 gtttggtagg aaagcatctg aaggagtagc aagagtttct gatagccttt taaatacaat
     6901 aaaattaaat agaaatgaag aaaattctaa aatgttggtt catttaacta aaataatgga
     6961 caagtttgat ttagacgatt ttcaagaaac taaggaacca aatttcgttc aaaaactttt
     7021 caaaaaagct aataatgcca ttgaaatgat gtttcaaaag tatgaaacat tgggaggaga
     7081 agttgagaaa attcaaattg agcttgaaca atacgaaaga gatatagcac tttctaataa
     7141 gcaaataggt gctatgttaa atgaaaactt tgagttttac aatgaacttc aaaaatatat
     7201 tgttgctgga gaaatggcaa tagaagaaat ggataatgaa atactacctt tctttaaaca
     7261 aaaatcagag actagtggag accaaatgga tgttgtcaat tatcaagaac tattaaaagt
     7321 ttatgatatg ttaaatcaaa gagtctatga tttaaggata gcagaaaata tagctatcca
     7381 aacaatacct atgcttagag gtatgcaaca taataattat ggtttaatta gaaaaataaa
     7441 tagtgcattt gtcgtaactt taccagtatt taaacaatgt ttatctcaag ctatattact
     7501 taaaaagcaa gaattacaag caaaatctct aaaagcactt gatgacaaaa ctaatgaatt
     7561 attgcttaga aatgcacaaa atgtaagtac tcaaagtgca caaatagcta gaatggctgg
     7621 aacaagttct gttcaaatag aaacattgga aaaaatgtac aatactatca agtctggtat
     7681 agatgaaact atgagaatag aagaaaacaa tagagcttta attaaggata atacaaagag
     7741 attggaagaa ttaaatacga ctataattta taataagtag tattatatta agggctaatt
     7801 tgtataaagt tggccctttc tatgtatatg aggaggctag acattgaaat atttcgatta
     7861 tatctgcaaa gatgatttgg agagaatttt tttgaaggag ccagaagatt ttagtgctaa
     7921 gactgaaaaa gatgtgttga aatatgcact gggcgctttt ttatacgttc cagctactca
     7981 atataatatg atttataaga gtattatagg ggatgttaag ggagttagac cattagctat
     8041 ttgtttagaa gatgctgttg gagtaaatgg agagttggag gctatagaaa atcttagatt
     8101 aattttaaaa aatattagca atgaaagtat aactaataaa gatggaatac cactaatttt
     8161 tgtaagaata aaagatgtgg agcaactgtt gagaattaaa gagattataa ttaaaaatag
     8221 tcattttata acaggaatat taattcctaa agcaaattcg gagttaattg aaaattgtat
     8281 agaagcttta gattttatga atcttcaaga tatgtatgtt attccgatag tagagacaaa
     8341 agaatttata tataatgaaa aaaaagaact tagttttact aatttgtaca atgctatttt
     8401 aagacataag tcaagaatat taagcattag agtaggcata actgatatat tagggatgta
     8461 tggtataagg agagataaaa acttttctat atataataat ttgatttgtt cttcatttat
     8521 attagatata atcacatatc ttcaaagacc agagttagat atacctataa gtggtggagt
     8581 atcagaattt tttgatatga cgaataagga tataagaaat aagtatatag aagaaatatt
     8641 attggataaa tatcatggat taattggaaa gaccgtaatt catccaatgc aaattcaaat
     8701 tgtacaagcg ctatctacag tatcatatga agattttact gatgctttgg atatactgga
     8761 tagtacagac agtaagtatg gtgtgagcaa gggtgtatta ggagagagaa tgaatgaaac
     8821 aaatccacat tttttatggg ctaaaaaaac attgattcta tctaaaatat atggtgtatt
     8881 aaataaagga gtagactatg aagaattgct taaattttaa ttgtgagatt gacattattg
     8941 ataatccatt gaatttagaa ctaaatgatt ttttagacgt aggaataaga aataatagca
     9001 aaagacgttt tttgtttatc tcaaagaaac tcggtaaaca tttagcatgt aagccaagtg
     9061 agatggataa tttgggaaaa ttaatggcta caatttataa tgaaaaaaat aaggaagaat
     9121 caaatggtac tgttatagct tttgctgaaa ctggtacagc tgttgggcat agtttttttg
     9181 attacttatg tggagattat gaatttattc atactactag agagcagttt gacgagttag
     9241 aaaatctgga atttttagag gaacattccc atgcaacaga ccagaattta tactttggta
     9301 tgcttaacaa ctttaaattt ggagatgaga taatattggt tgatgatgag attaccacag
     9361 ccaatacatg catgaatata attaggaaaa ttcaaagcat ttatcctaaa aagaaataca
     9421 ctatatgctc aatactcaat tgggtaagtg atgaaaattt aagtaaagtt actcgtttag
     9481 aaagagagct agattgtaag atagattttg tatacttatt taaaggaaac tttaaatttt
     9541 atatagatga agatattatt tcaaattaca acgataatta tagtattact aaattagaga
     9601 ctatttcaag tgataaagta aatttagtag taaatcatat ttatgttgat ttagaagatt
     9661 atattggaaa taagaaatat gtaaaatata caggaagatt tggaattaat agaagagaac
     9721 aatttagatt aaaagaaatc gttaagagag aaagtaaaaa aataactatg gccagtaaag
     9781 aatctttaaa aaatgaaaaa gaaaagattc tatttttagg tactgaagaa tttatgtata
     9841 tacctatgct gtttgcaaaa gaaaaagaaa atcaatatga tatatactat cattcaacaa
     9901 caagaagtcc aattattaat attgataaga ataattatcc tattaaaagt aaatttatgc
     9961 taaagagttt ttataatata gatattcaaa attatattta caatatagat aaacataatt
    10021 tttctaagtg ttatttattt gtagagctga ataaaagtga aaattcttat ctagaattta
    10081 ttgatataat taaaaaaaca agtataaaag aattaactat agtatgttgt agctaaagtg
    10141 ttttaacagg ataaaatata tcacgatttt aagataggag ttgagtcata tgagagattt
    10201 tagcacgtat tctaaagatg atgttatttt tttattaaaa gatatatcaa atttaattga
    10261 agaagaagga aataaagaaa gagaattaaa aatacaaaat ggtagacatt attcagaaat
    10321 gataccaata gagtatgaag ttagtgaaga ttatctgaat ttatatcatg agaaactaag
    10381 agaaaataaa gataaacttg cattttctat aggagttatg tgtgaaaaaa taattaaaaa
    10441 aaatggaaat gatgtagtac tagtttcact tgctagagca ggaacaccta ttggtatttt
    10501 ggcaaaaaga tatataagaa gcaagtataa tataagttta cctcactata caatctctat
    10561 aataagagat aagggaatag atatgaatgc aattgaatat atagtaaaga atcatccgag
    10621 ctcaaaaatt caatttttag atggatggac tggtaaggga actatatcaa aagaacttga
    10681 aaaagcatgt gatgaactta atgttaaatt taaaaaatat tttgactcaa cacttgctgt
    10741 attagctgac cctgctggat attcaggact ttatggaatt agagaagatt tcttaatacc
    10801 aagtgcatgt ttaaattcaa cagtttctgg tttagtaagt agaactgtac taagagagga
    10861 tttaattggt aaaaatgact tccatggagc taagttttat agacatttaa aagataaaga
    10921 tgaatctatg aactatatcg aaactataga agaatgtttt aaaaatcaat ttaagaatat
    10981 atctgatgaa gttgaaaact gggaaagtga cattataaca agagatggat actttgatgt
    11041 tttaaatata aaagaaaagt ataacataac agatattaac tttatcaaac caggtgtagg
    11101 ggaaacgaca agggttttac ttagaagagt tccgtataag attcttgtta aagacttaaa
    11161 tgataaatct ttagaccata tattcatatt agctaaggag aaaaatgttg aagttgaaca
    11221 aatggattta aaagcctata aatgttgtgg aataataaaa aatatgaaag atatttagga
    11281 aatatattag acaggagaat attgaatgat tgttttttca gatttagaca ggagtatcat
    11341 ttactccaat aaatttttaa atgctgatag taaatatact aatatagaaa tttataggga
    11401 aaaagaaata tcttatatat ctttagatac tataaattta ataaagcaaa tacaatatta
    11461 tggtatgttt atacctacaa ctactagaac agtggagcaa tttaagcgta tagaatttaa
    11521 taaatatggg atttattttc cttggagtat aacttcaaat ggaggagtta tacttaaaga
    11581 taatgaaata ttgaaatcat ggagtgaaaa aatagataaa ctaaaatgtg attatgagcc
    11641 cattgaaagt atgattcata aatttaaaga ctatttgaat gtagatggaa taactaattt
    11701 taaagttgca gaagatacat ttttttatat agttgttgat ttatcaagat ttaatttgga
    11761 ttcaatcaaa gaatatacaa atatattgga atcaaaaaat tggaagtttt atgtgagtgg
    11821 cagaaaaata tattttatac caaaagaaat ttcaaaagaa aatgctataa aatacttgac
    11881 taaagaatta ggaatagaat atttttatgc agttggagat tctattatgg actatggtat
    11941 gcttaatatc tcaaataaat cctatgtatt gaagcatggt gatataaata aaaatgaaat
    12001 agaaaattca tttatatcaa gttctttcaa tggaatgagt ggaactgaag agatactatc
    12061 taacatttta gacgagaact gcctacttaa tatataatga ctgatatata aataataaac
    12121 tagaaaaatc tgaaataaat tatagtaaag tttatcatac taatttattt cagattttta
    12181 tcaatttata tttaagtaag aactcaatat gttaaaatga aggtggagtc attgcccaga
    12241 ctgagataca tacctcatca tcataatttt catacacatg tggtatagta ctgttaaaat
    12301 aaaaactatc tccttcctcc aatagatatt ccttatttcc aagtgttatc ttcattttac
    12361 cttttattat atagccacat tcttctcctt cgtgtgtcac aaattcactg tttttttgtg
    12421 tatgaccatc tagtgtaaca agtataaatt ctattttttt cccagataaa ttaggaacaa
    12481 gaagttcata aaattttgta gaatcattag ttactatttt ttttcgatta tttttcttta
    12541 ctactatatc agagtcttct cttccaatct cttcaaaaaa ataacctata tttatatcaa
    12601 gagcttttga tattttccac attgctacaa tagatggaac aaccatatta cgctcaattt
    12661 gacttattaa gcctgtactt aaatctgaat tttttgctaa agtagagata cttatatttt
    12721 tagattttct aagagatcta attttgcttc caacatctat attcattaga tacaaacccc
    12781 ctcagacatg attataacat ataatttata aagattaata aaataaaaaa aaagttttac
    12841 aatacaagta ttttttatgc tatattgaat tttctgaaaa gatgcattat aatagtatta
    12901 atattcaata tattgaatat tgataacgat attttaattt aatgaaatgg aggtaattaa
    12961 gatgaagagt tttgtaagtc ctgataatat ttgggtatta tgggcagttg taactggatg
    13021 ggcagcattt agtatttatt tggaacaaaa gtacaattgg gcatcaaaag tgtcgggagc
    13081 aataatagct cttgtaggag caatgttatt atcaaatcta aatattatac cagtagagtc
    13141 tgttgtatat gaccaagtat ggggatatgt tgttccttta gccatagctt tattgttata
    13201 tcaatgtaac attaaaaaga tatggaaaga aagtggaaga ttgctcatta tatttcttgt
    13261 aggttctgta ggaactgtac ttggagctat gattggattt ttagcattaa aaaatgtagt
    13321 accagattta aatattgttg cagctatgat gacaggttct tatataggtg gaaatgtaaa
    13381 ctttgctgct atgtcagggg cgtttgatgc gccaggagaa ttagtatcag caacagttgt
    13441 agcagataat cttttaatgg cattatattt ctttgtctta atagctatac catcaattgg
    13501 attttttaga aaacatttta aacatccaca tgtagatgaa atggaaagta taggtataac
    13561 tgaaggagaa acagtagcag caaattattg gggaagaaaa gaaatatctt taaaagatat
    13621 agcaatagcc attggtagcg cctttataat tgttgcagta tctgtagaac tatcaacttg
    13681 gtttaaagct ataataccat tatcaaatcc ttttttagca atgttaaata ctttatttgg
    13741 aaatcaatat ctaataataa ctactttgac tatgttgttt gccacattta tgcctaattt
    13801 ctttggtgat ataaaaggag cacaggaatt gggtacattt ttaatatata tattctttgt
    13861 tgtagtaggt gttcctgctt caataacatt aataattcaa aaatcaccat tactactttt
    13921 atactgcgga ataatggtac taataaacat gctagttaca tttatagttg caaaaatatt
    13981 taagtttagt ttagaagaag ctattcttgc atctaatgca aatattggtg gtcctacaac
    14041 agcagcagct atggcaatat caaaaggatg gtctaagcta gtagggccaa tacttatagt
    14101 tggaacattt ggatacatag tcggtaatta ttttggtctt ttagtaggaa atattttgat
    14161 ataattttac gtgaaaaaaa gttgtttatt agaaagattt ttatttaatt ggaggttgtt
    14221 atgattttcg atggacattc agatacttgg gcagatgtaa caataagaag gcttaacggg
    14281 gaaaaagatg tatttagaag aatccatctt gataatttta aaagagcaaa tatagaagga
    14341 gctatttttg taatatggat agacccacca tatgataaag agcctgaaaa aagagttaaa
    14401 caaattataa gttctataaa agatgaaatt gaagatatga aagatgttgt aaacatagta
    14461 aaaaaatatt cagatataga taaaggtata agtgaaaaca aaataaatat tttaattggt
    14521 cttgaaggat taagccatat tggaagaaat atagatttac tagattattt ttataaagaa
    14581 gttggtgcaa gacatgcatc attaacttgg aatgaacaga atgaacttgc tacaggatgt
    14641 ttaggggatg aaaatcgtgg tttaagtgaa ataggcaaaa aagcagtcaa taaaatggaa
    14701 aaattgggca tgatagtaga tgtgtcacat ttaaatgaaa aatcattttg ggatttaatg
    14761 tctgttgcaa gtaagccagt tatagcttct cattctaatg ctaggagttt atgtgatgct
    14821 aaaagaaatc ttacagataa tcaattaaaa gaaatttcaa atacaggtgg attagttgga
    14881 ataaatagtt ttagaggatt tactaatagt aatcctaaga agcaagattt aatacatttg
    14941 gttgaccata tagactatat ggtaaatctt ataggtatag aacatgtatc ttttggtttt
    15001 gattttagtg attacttagg gaaagagact ataggttctt ttactggaaa tgctagtgtg
    15061 ccaagtgtag atggtcttga aagcacacat acagcacaga acatagtagt agagcttgaa
    15121 aatagaggtt ataaaaagaa agatatagag ttaatctcta gtggtaattt ttataggctc
    15181 tttaaagata tactaaaata atataataaa gaacttaaat ttataaaata gtatatttat
    15241 acaaataata tctacatatt taaattggcg ttcataattt attaaatttt aatgttacta
    15301 gaagtttcaa ttctagtaac attttttatg ttaaaaacag atatagatat gtattgacaa
    15361 tgataattat tattgataaa ctataaaagt gaaaatgata ataattttca ttttaattaa
    15421 taaaagattt ttaaggaggg agaatgaaga aaaggtattt agtcatagga ataataattt
    15481 tgtcttattt ttctttattt ataggagctg aagatataaa tataatgcat atatttgcca
    15541 aaaatcagca taaacttatg atatttatca tgagtagaat accaagatta ataagtatat
    15601 tgattgctgg tgtaggaatg agcatagcag gtcttataat gcaacagata agtaagaata
    15661 aatttgtatc acctacaaca ggtgctacaa tagatgcagc acaatttggg atagtaatat
    15721 gtatgctttt agtaccaact gcctctattt ttacaaagac aataatagca tttgtatttt
    15781 ctcttgtagg gacttttacg tttatgaaaa taatagggaa gttacaattt aaaaatataa
    15841 tctttgtacc attagtaggt ataatgtttg gaaatataat agggtctatg acggatttta
    15901 tcgcgtataa atacgatttg agtcaaaatg ttagttcttg gatgcaaggt gacttttcta
    15961 tgatacttaa aggaaattat gagatattat atatcacaat accccttata atattagcat
    16021 atatttatgc aaataaattt actgttgttg gtatgggtat ggattttgcg acaaacttag
    16081 gtcttagtta caaaagaatt gtcaatatag gtctgataat agtagcactt gttacagtat
    16141 gtgtagtcgt tacagctggt aatatccctt ttataggtct aatagttcca aatatcgtat
    16201 ccttgtacat gggtgataat ataagagcaa gcatatggta tactggttta cttggagcga
    16261 tatttgtact tatatgtgat atttttggaa gaattattat ttatccatat gaaatatcta
    16321 tagggcttac agttggagtt ataggaagta tactattcct ttatttaata ttaaggagga
    16381 atgtgaatga agcttagtct caatcaaaaa atttactttg taagtatatt actattcata
    16441 tcaatggcac tttttataat ttatggaata gacatgaatc atttagaata tgcattatca
    16501 caaagaatac caaaaatatt tgctatggta ttaggtggag gatgtatagc atttactact
    16561 gtagtgtttc aaacaattac aaacaatcaa atattaacac caagtgtact aggtttagat
    16621 tcattgtatg taatgattca aactataata gtattcatat ttggttcaag cagtagtctg
    16681 attataaatg aaaattataa ttttattatt aatgtagcta tgatgattgg agcttcattg
    16741 ttattatata agacgttatt tgaaaaaaat aaaaataata ttttcttttt aatcctagta
    16801 ggaatgatat ttggaacctt atttaaaagt gcaacaacat ttattcaggt aatgatagac
    16861 cctaatgaat ttttagcttt acaaactagt ataatggcaa gtttaaacaa tataaacact
    16921 aatgtattgt taatagcatt tataataatt attgccataa taccttttat atatgatgaa
    16981 ataaagtatt tagatgtatt gtcgttaggt aaagaacaag caattaattt aggtgtagat
    17041 tttgataaag cagttaaaaa aatgataata ttaatagcaa ttttagtctc aatatcaaca
    17101 gcacttattg gacctatgac atttttagga cttttacttg ctaatataac aagagaaatt
    17161 tttaaaacat ataagcatac atacttgatt tcgggttcta tgttgatagg gatgattaca
    17221 ttgataatag gtcaattttt tatacaacat gtatttaagt ttgatacaac tttaagtgta
    17281 gtaataaatt ttataggagg aatttatttt attcaattac tattaaaggg ggcaaataga
    17341 tgatagagat aaaaaatata tttaaaagat ataagaataa aaatgttgta gatgatgtct
    17401 cttttagtat agaaaaggga aagataacat cttttatagg accaaatgga gctggaaaaa
    17461 gtacagtact atcaatagtt acaagactta taggaggtga tggtggagaa gtaataatag
    17521 aaggaaaaag cttgactaat tatagcaata aagaattagc aaaaaaaata gctattctta
    17581 aacaatctaa caatataact ttaaagctta caataagaga attagttgga tttggaagat
    17641 ttccatatag tgaaggtaat ctaactaaag aggatgaaaa ttatattgat gaagcaatag
    17701 aatatatgaa gttgactgat atacaacata agtatttaga tgaattgagt ggaggtcaaa
    17761 gacaaagagc ttatatagca atggtaatag ctcaagatac agagtacata ttacttgatg
    17821 aaccactgaa caatttggat atgaaccatt ccgtacagat gatgaaggta cttagaggtc
    17881 tttgtgatga attagataag actatagtat tagtaatgca tgatataaat tttgcatcat
    17941 gttattcaga taatatagtt gcattaaaaa atggaaaagt tgaaaaagta ggtcgtacag
    18001 atgagatagt aaatgaaaaa gttttagaag atatttatga gatgaatttt aacattaaga
    18061 atataaatgg aaatcgaatc tgtatttatt tttaaaatat tgtatatgaa aataaacttg
    18121 ctatatgatt ttttataaag tttttaaatt aaaataaaga ttaataggag agaggaaaat
    18181 attgatatga ataaaaaagc agcaatagta gcagcagtag ctattatagg attagtgaca
    18241 gtgtttgcat taggtggaag taaaaagaat gaatcaaaaa catcagaaaa ttcaaataat
    18301 actatgaaga taactcataa cttgggagaa acagatgtaa aattaaatcc taaaaaagta
    18361 gtggtatttg attattcggc tttagataca atggatgcat taggtgtagc tgagacttta
    18421 gtaggacttc caaaagcgag tttaccagca tccttagaaa aatataagga tgacaagtat
    18481 gcagatttag gaggattaaa agaaccagat ttagaaggta taaagtcggc aaatccagat
    18541 ttaattataa taaatggaag acaagaagat ttttatgagc aattgtcaaa aatagcacca
    18601 acaataagta caagtaaaga tgataagaag tacttagaat ctgttaaaaa taatatagac
    18661 aaaatagcaa aaatatttgg agtagaagaa aaagcaaatc aagaatttag taaaattgaa
    18721 aagaaaatag aaactttgaa taaaaaagta acagataaaa atttaaatgc tttaactata
    18781 atggtaaatg aaggtaattt aagtgtattt ggtgaagaat caagatttag tatattatac
    18841 aatagttttg gatttgaaaa taaagataaa aatataaaag aatcaagtca tggacaaaat
    18901 attacttttg aatatatagc taaacaaaat ccagaagtta tgttcgttat agatagagga
    18961 atagctactg gcagtgatgt aaaagaaagc tcaactgcaa aatctgtttt gaacaatgat
    19021 ataataaaat ctatggatgc atataagaat gataatataa tatatttaga ttcaccaaca
    19081 tggtatgtaa atgatggtgg tctaacatct ttaaataaaa tgatagatga tgcttcaaaa
    19141 gcggtaaatt aataatccga tataaccaaa atgtattttt tataaaatga ttatcaaata
    19201 aactaggagt ttttaagaac tcctagttta ttttttgcat aaaaaagata tagtttaaac
    19261 aataatatta tctataataa gataaa
//
LOCUS       DAMJYR010000063        18824 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.063, whole genome shotgun sequence.
ACCESSION   DAMJYR010000063 DAMJYR010000000
VERSION     DAMJYR010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 18824)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18824)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18824
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.063"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            111..665
                     /gene="tcdR"
                     /locus_tag="PC471_002257"
     CDS             111..665
                     /gene="tcdR"
                     /locus_tag="PC471_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosylating toxin sigma factor TcdR"
                     /protein_id="HDF2789385.1"
                     /translation="MQKSFYELIVLARNNSVDDLQEILFMFKPLVKKLSRVLHYEEGE
                     TDLIIFFIELIKNIKLSSFSEKSDAIIVKYIHKSLLNKTFELSRRYSKMKFNFVEFDE
                     NILNMKNNYQSKSIFEEDICFFGYILKELSGIQRKVIFYKYLKGYSDREISVKLKISR
                     QAVNKAKNRAFKKIKKDYENYFNL"
     gene            983..8083
                     /gene="tcdB"
                     /locus_tag="PC471_002258"
                     /gene_synonym="toxB"
     CDS             983..8083
                     /gene="tcdB"
                     /locus_tag="PC471_002258"
                     /gene_synonym="toxB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_074049122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosylating toxin TcdB"
                     /protein_id="HDF2789386.1"
                     /translation="MSLVNRKQLEKMANVRFRTQEDEYVAILDALEEYHNMSENTVVE
                     KYLKLKDINSLTDIYIDTYKKSGRNKALKKFKEYLVTEVLELKNNNLTPVEKNLHFVW
                     IGGQINDTAINYINQWKDVNSDYNVNVFYDSNAFLINTLKKTVVESAINDTLESFREN
                     LNDPRFDYNKFFRKRMEIIYDKQKNFINYYKAQREENPELIIDDIVKTYLSNEYSKEI
                     DELNTYIEESLNKITQNSGNDVRNFEEFKNGESFNLYEQELVERWNLAAASDILRISA
                     LKEIGGMYLDVDMLPGIQPDLFESIEKPSSVTVDFWEMTKLEAIMKYKEYIPEYTSEH
                     FDMLDEEVQSSFESVLASKSDKSEIFSSLGDMEASPLEVKIAFNSKGIINQGLISVKD
                     SYCSNLIVKQIENRYKILNNSLNPAISEDNDFNTTTNTFIDSIMAEANADNGRFMMEL
                     GKYLRVGFFPDVKTTINLSGPEAYAAAYQDLLMFKEGSMNIHLIEADLRNFEISKTNI
                     SQSTEQEMASLWSFDDARAKAQFEEYKRNYFEGSLGEDDNLDFSQNIVVDKEYLLEKI
                     SSLARSSERGYIHYIVQLQGDKISYEAACNLFAKTPYDSVLFQKNIEDSEIAYYYNPG
                     DGEIQEIDKYKIPSIISDRPKIKLTFIGHGKDEFNTDIFAGFDVDSLSTEIEAAIDLA
                     KEDISPKSIEINLLGCNMFSYSINVEETYPGKLLLKVKDKISELMPSISQDSIIVSAN
                     QYEVRINSEGRRELLDHSGEWINKEESIIKDISSKEYISFNPKENKITVKSKNLPELS
                     TLLQEIRNNSNSSDIELEEKVMLTECEINVISNIDTQIVEERIEEAKNLTSDSINYIK
                     DEFKLIESISDALCDLKQQNELEDSHFISFEDISETDEGFSIRFINKETGESIFVETE
                     KTIFSEYANHITEEISKIKGTIFDTVNGKLVKKVNLDTTHEVNTLNAAFFIQSLIEYN
                     SSKESLSNLSVAMKVQVYAQLFSTGLNTITDAAKVVELVSTALDETIDLLPTLSEGLP
                     IIATIIDGVSLGAAIKELSETSDPLLRQEIEAKIGIMAVNLTTATTAIITSSLGVASG
                     FSILLVPLAGISAGIPSLVNNELVLRDKATKVVDYFKHVSLVETEGVFTLLDDKIMMP
                     QDDLVISEIDFNNNSIVLGKCEIWRMEGGSGHTVTDDIDHFFSAPSITYREPHLSIYD
                     VLEVQKEELDLSKDLMVLPNAPNRVFAWETGWTPGLRSLENDGTKLLDRIRDNYEGEF
                     YWRYFAFIADALITTLKPRYEDTNIRINLDSNTRSFIVPIITTEYIREKLSYSFYGSG
                     GTYALSLSQYNMGINIELSESDVWIIDVDNVVRDVTIESDKIKKGDLIEGILSTLSIE
                     ENKIILNSHEINFSGEVNGSNGFVSLTFSILEGINAIIEVDLLSKSYKLLISGELKIL
                     MLNSNHIQQKIDYIGFNSELQKNIPYSFVDSEGKENGFINGSTKEGLFVSELPDVVLI
                     SKVYMDDSKPSFGYYSNNLKDVKVITKDNVNILTGYYLKDDIKISLSLTLQDEKTIKL
                     NSVHLDESGVAEILKFMNRKGNTNTSDSLMSFLESMNIKSIFVNFLQSNIKFILDANF
                     IISGTTSIGQFEFICDENDNIQPYFIKFNTLETNYTLYVGNRQNMIVEPNYDLDDSGD
                     ISSTVINFSQKYLYGIDSCVNKVVISPNIYTDEINITPVYETNNTYPEVIVLDANYIN
                     EKINVNINDLSIRYVWSNDGNDFILMSTSEENKVSQVKIRFVNVFKDKTLANKLSFNF
                     SDKQDVPVSEIILSFTPSYYEDGLIGYDLGLVSLYNEKFYINNFGMMVSGLIYINDSL
                     YYFKPPVNNLITGFVTVGDDKYYFNPINGGAASIGETIIDDKNYYFNQSGVLQTGVFS
                     TEDGFKYFAPANTLDENLEGEAIDFTGKLIIDENIYYFDDNYRGAVEWKELDGEMHYF
                     SPETGKAFKGLNQIGDYKYYFNSDGVMQKGFVSINDNKHYFDDSGVMKVGYTEIDGKH
                     FYFAENGEMQIGVFNTEDGFKYFAHHNEDLGNEEGEEISYSGILNFNNKIYYFDDSFT
                     AVVGWKDLEDGSKYYFDEDTAEAYIGLSLINDGQYYFNDDGIMQVGFVTINDKVFYFS
                     DSGIIESGVQNIDDNYFYIDDNGIVQIGVFDTSDGYKYFAPANTVNDNIYGQAVEYSG
                     LVRVGEDVYYFGETYTIETGWIYDMENESDKYYFNPETKKACKGINLIDDIKYYFDEK
                     GIMRTGLISFENNNYYFNENGEMQFGYINIEDKMFYFGEDGVMQIGVFNTPDGFKYFA
                     HQNTLDENFEGESINYTGWLDLDEKRYYFTDEYIAATGSVIIDGEEYYFDPDTAQLVI
                     SE"
     gene            8205..8705
                     /gene="tcdE"
                     /locus_tag="PC471_002259"
     CDS             8205..8705
                     /gene="tcdE"
                     /locus_tag="PC471_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin-like glycosylating toxin export protein
                     TcdE"
                     /protein_id="HDF2789387.1"
                     /translation="MHSSSPFYISNGNKIFFYINLGGVMNMTISFLSEHIFIKLVILT
                     ISFDTLLGCLSAIKSRKFNSSFGIDGGIRKVAMIACIFFLSVVDILTKFNFLFMLPQD
                     CINFLRLKHLGISEFFSILFILYESVSILKNMCLCGLPVPKRLKEKIAILLDAMTDEM
                     NAKDEK"
     gene            9010..9216
                     /locus_tag="PC471_002260"
     CDS             9010..9216
                     /locus_tag="PC471_002260"
                     /inference="COORDINATES: protein motif:HMM:NF022413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2789388.1"
                     /translation="MKLFKNTKIRDEGQNDIENKYQTLINDLIDSLKVIGKIYKEIKT
                     LKKYKICLQITIRQSPYLIEESIN"
     gene            9433..17565
                     /gene="tcdA"
                     /locus_tag="PC471_002261"
                     /gene_synonym="toxA"
     CDS             9433..17565
                     /gene="tcdA"
                     /locus_tag="PC471_002261"
                     /gene_synonym="toxA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_105161403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosylating toxin TcdA"
                     /protein_id="HDF2789389.1"
                     /translation="MSLISKEELIKLAYSIRPRENEYKTILTNLDEYNKLTTNNNENK
                     YLQLKKLNESIDVFMNKYKTSSRNRALSNLKKDILKEVILIKNSNTSPVEKNLHFVWI
                     GGEVSDIALEYIKQWADINAEYNIKLWYDSEAFLVNTLKKAIVESSTTEALQLLEEEI
                     QNPQFDNMKFYKKRMEFIYDRQKRFINYYKSQINKPTVPTIDDIIKSHLVSEYNRDET
                     VLESYRTNSLRKINSNHGIDIRANSLFTEQELLNIYSQELLNRGNLAAASDIVRLLAL
                     KNFGGVYLDVDMLPGIHSDLFKTISRPSSIGLDRWEMIKLEAIMKYKKYINNYTSENF
                     DKLDQQLKDNFKLIIESKSEKSEIFSKLENLNVSDLEIKIAFALGSVINQALISKQGS
                     YLTNLVIEQVKNRYQFLNQHLNPAIESDNNFTDTTKIFHDSLFNSATAENSMFLTKIA
                     PYLQVGFMPEARSTISLSGPGAYASAYYDFINLQENTIEKTLKASDLIEFKFPENNLS
                     QLTEQEINSLWSFDQASAKYQFEKYVRDYTGGSLSEDNGVDFNKNTALDKNYLLNNKI
                     PSNNVEEAGSKNYVHYIIQLQGDDISYEATCNLFSKNPKNSIIIQRNMNESAKSYFLS
                     DDGESILELNKYRIPERLKNKEKVKVTFIGHGKDEFNTSEFARLSVDSLSNEISSFLD
                     TIKLDISPKNVEVNLLGCNMFSYDFNVEETYPGKLLLSIMDKITSTLPDVNKNSITIG
                     ANQYEVRINSEGRKELLAHSGKWINKEEAIMSDLSSKEYIFFDSIDNKLKAKSKNIPG
                     LASISEDIKTLLLDASVSPDTKFILNNLKLNIESSIGDYIYYEKLEPVKNIIHNSIDD
                     LIDEFNLLENVSDELYELKKLNNLDEKYLISFEDISKNNSTYSVRFINKSNGESVYVE
                     TEKEIFSKYSEHITKEISTIKNSIITDVNGNLLDNIQLDHTSQVNTLNAAFFIQSLID
                     YSSNKDVLNDLSTSVKVQLYAQLFSTGLNTIYDSIQLVNLISNAVNDTINVLPTITEG
                     IPIVSTILDGINLGAAIKELLDEHDPLLKKELEAKVGVLAINMSLSIAATVASIVGIG
                     AEVTIFLLPIAGISAGIPSLVNNELILHDKATSVVNYFNHLSESKKYGPLKTEDDKIL
                     VPIDDLVISEIDFNNNSIKLGTCNILAMEGGSGHTVTGNIDHFFSSPSISSHIPSLSI
                     YSAIGIETENLDFSKKIMMLPNAPSRVFWWETGAVPGLRSLENDGTRLLDSIRDLYPG
                     KFYWRFYAFFDYAITTLKPVYEDTNIKIKLDKDTRNFIMPTITTNEIRNKLSYSFDGA
                     GGTYSLLLSSYPISTNINLSKDDLWIFNIDNEVREISIENGTIKKGKLIKDVLSKIDI
                     NKNKLIIGNQTIDFSGDIDNKDRYIFLTCELDDKISLIIEINLVAKSYSLLLSGDKNY
                     LISNLSNTIEKINTLGLDSKNIAYNYTDESNNKYFGAISKTSQKSIIHYKKDSKNILE
                     FYNDSTLEFNSKDFIAEDINVFMKDDINTITGKYYVDNNTDKSIDFSISLVSKNQVKV
                     NGLYLNESVYSSYLDFVKNSDGHHNTSNFMNLFLDNISFWKLFGFENINFVIDKYFTL
                     VGKTNLGYVEFICDNNKNIDIYFGEWKTSSSKSTIFSGNGRNVVVEPIYNPDTGEDIS
                     TSLDFSYEPLYGIDRYINKVLIAPDLYTSLININTNYYSNEYYPEIIVLNPNTFHKKV
                     NINLDSSSFEYKWSTEGSDFILVRYLEESNKKILQKIRIKGILSNTQSFNKMSIDFKD
                     IKKLSLGYIMSNFKSFNSENELDRDHLGFKIIDNKTYYYDEDSKLVKGLININNSLFY
                     FDPIEFNLVTGWQTINGKKYYFDINTGAALTSYKIINGKHFYFNNDGVMQLGVFKGPD
                     GFEYFAPANTQNNNIEGQAIVYQSKFLTLNGKKYYFDNDSKAVTGWRIINNEKYYFNP
                     NNAIAAVGLQVIDNNKYYFNPDTAIISKGWQTVNGSRYYFDTDTAIAFNGYKTIDGKH
                     FYFDSDCVVKIGVFSTSNGFEYFAPANTYNNNIEGQAIVYQSKFLTLNGKKYYFDNNS
                     KAVTGWQTIDSKKYYFNTNTAEAATGWQTIDGKKYYFNTNTAEAATGWQTIDGKKYYF
                     NTNTAIASTGYTIINGKHFYFNTDGIMQIGVFKGPNGFEYFAPANTDANNIEGQAILY
                     QNEFLTLNGKKYYFGSDSKAVTGWRIINNKKYYFNPNNAIAAIHLCTINNDKYYFSYD
                     GILQNGYITIERNNFYFDANNESKMVTGVFKGPNGFEYFAPANTHNNNIEGQAIVYQN
                     KFLTLNGKKYYFDNDSKAVTGWQTIDGKKYYFNLNTAEAATGWQTIDGKKYYFNLNTA
                     EAATGWQTIDGKKYYFNTNTFIASTGYTSINGKHFYFNTDGIMQIGVFKGPNGFEYFA
                     PANTHNNNIEGQAILYQNKFLTLNGKKYYFGSDSKAVTGLRTIDGKKYYFNTNTAVAV
                     TGWQTINGKKYYFNTNTSIASTGYTIISGKHFYFNTDGIMQIGVFKGPDGFEYFAPAN
                     TDANNIEGQAIRYQNRFLYLHDNIYYFGNNSKAATGWVTIDGNRYYFEPNTAMGANGY
                     KTIDNKNFYFRNGLPQIGVFKGSNGFEYFAPANTDANNIEGQAIRYQNRFLHLLGKIY
                     YFGNNSKAVTGWQTINGKVYYFMPDTAMAAAGGLFEIDGVIYFFGVDGVKAPGIYG"
     gene            complement(17900..18598)
                     /gene="tcdC"
                     /locus_tag="PC471_002262"
     CDS             complement(17900..18598)
                     /gene="tcdC"
                     /locus_tag="PC471_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosylating toxin anti-sigma factor TcdC"
                     /protein_id="HDF2789390.1"
                     /translation="MFSKKNEGNEFSNEGKGSSKKIIKFFKSTKGIALLAFILGVFFG
                     NISSPACSEDHEEVISNQTSVIDSQKTEIETLNSKLSDAEPWFKMKDDEKKAIEAENQ
                     RKAEEAKKAEEAKKAEEQRKKEEEEKKGYDTGITYDQLARTPDDYKYKKVKFEGKVIQ
                     VIEDGDEVQIRLAVSGNYDKVVLCSYKKSITPSRVLEDDYITIRGISAGTITYESTMG
                     GNITIPGIAVEKIN"
BASE COUNT         7448 a         2074 c         2908 g         6394 t
ORIGIN      
        1 ataaaattta atttatttgc cgattatata attataatga ctgatttaat tccaatgttg
       61 tcaaaatttt caaataaatc atcattataa atataagaga ggatgatttt atgcaaaagt
      121 ctttttatga attaattgtt ttagcaagaa ataactcagt agatgatttg caagaaattt
      181 tatttatgtt taagccatta gtaaaaaaac ttagtagagt tttacattat gaagagggag
      241 aaacagattt aataatattt tttattgaat taataaaaaa tattaaatta agtagctttt
      301 cagaaaaaag cgatgctatt atagtcaaat atattcataa atcattactg aataagactt
      361 ttgagttgtc tagaagatat tctaaaatga agtttaattt tgtagaattt gatgaaaata
      421 tcttaaatat gaaaaataat tatcaaagta agtctatttt tgaggaagat atttgttttt
      481 tcggatatat tttgaaagaa ttatctggta ttcaaagaaa agttattttt tataaatatt
      541 taaaaggata ttctgataga gaaatatcag tgaaattaaa aatatctaga caagctgtta
      601 ataaggctaa aaatagagca tttaaaaaaa taaaaaaaga ctatgaaaat tattttaact
      661 tgtaattaat gagtttaaag aaatatttac aatagaaatc aaattttata attaacttta
      721 ttgtaaaatt aataacttaa tctaagaata tcttaatttt tatattttat atagaacaat
      781 gtttacatat ttatttcaga caacgtcttt attcaatcga agagcaaatt aatcaactga
      841 gtgtcttaaa tttaaaatgt taggaagtga atgtatatga aaacctaagt agatattagt
      901 atattttata aatagaaagg aggatatata aaagagtttt agcatttaga tgtaaaaata
      961 ttatagtaaa ggagaaaatt ttatgagttt agttaataga aaacagttag aaaaaatggc
     1021 aaatgtaaga tttcgtactc aagaagatga atatgttgca atattggatg ctttagaaga
     1081 atatcataat atgtcagaga atactgtagt cgaaaaatat ttaaaattaa aagatataaa
     1141 tagtttaaca gatatttata tagatacata taaaaaatct ggtagaaata aagccttaaa
     1201 aaaatttaag gaatatctag ttacagaagt attagagcta aagaataata atttaactcc
     1261 agttgagaaa aatttacatt ttgtttggat tggaggtcaa ataaatgaca ctgctattaa
     1321 ttatataaat caatggaaag atgtaaatag tgattataat gttaatgttt tttatgatag
     1381 taatgcattt ttgataaaca cattgaaaaa aactgtagta gaatcagcaa taaatgatac
     1441 acttgaatca tttagagaaa acttaaatga ccctagattt gactataata aattcttcag
     1501 aaaacgtatg gaaataattt atgataaaca gaaaaatttc ataaactact ataaagctca
     1561 aagagaagaa aatcctgaac ttataattga tgatattgta aagacatatc tttcaaatga
     1621 gtattcaaag gagatagatg aacttaatac ctatattgaa gaatccttaa ataaaattac
     1681 acagaatagt ggaaatgatg ttagaaactt tgaagaattt aaaaatggag agtcattcaa
     1741 cttatatgaa caagagttgg tagaaaggtg gaatttagct gctgcttctg acatattaag
     1801 aatatctgca ttaaaagaaa ttggtggtat gtatttagat gttgatatgt taccaggaat
     1861 acaaccagac ttatttgagt ctatagagaa acctagttca gtaacagtgg atttttggga
     1921 aatgacaaag ttagaagcta taatgaaata caaagaatat ataccagaat atacctcaga
     1981 acattttgac atgttagacg aagaagttca aagtagtttt gaatctgttc tagcttctaa
     2041 gtcagataaa tcagaaatat tctcatcact tggtgatatg gaggcatcac cactagaagt
     2101 taaaattgca tttaatagta agggtattat aaatcaaggg ctaatttctg tgaaagactc
     2161 atattgtagc aatttaatag taaaacaaat cgagaataga tataaaatat tgaataatag
     2221 tttaaatcca gctattagcg aggataatga ttttaatact acaacgaata cctttattga
     2281 tagtataatg gctgaagcta atgcagataa tggtagattt atgatggaac taggaaagta
     2341 tttaagagtt ggtttcttcc cagatgttaa aactactatt aacttaagtg gccctgaagc
     2401 atatgcggca gcttatcaag atttattaat gtttaaagaa ggcagtatga atatccattt
     2461 gatagaagct gatttaagaa actttgaaat ctctaaaact aatatttctc aatcaactga
     2521 acaagaaatg gctagcttat ggtcatttga cgatgcaaga gctaaagctc aatttgaaga
     2581 atataaaagg aattattttg aaggttctct tggtgaagat gataatcttg atttttctca
     2641 aaatatagta gttgacaagg agtatctttt agaaaaaata tcttcattag caagaagttc
     2701 agagagagga tatatacact atattgttca gttacaagga gataaaatta gttatgaagc
     2761 agcatgtaac ttatttgcaa agactcctta tgatagtgta ctatttcaga aaaatataga
     2821 agattcagaa attgcatatt attataatcc tggagatggt gaaatacaag aaatagacaa
     2881 gtataaaatt ccaagtataa tttctgatag acctaagatt aaattaacat ttattggtca
     2941 tggtaaagat gaatttaata ctgatatatt tgcaggtttt gatgtagatt cattatccac
     3001 agaaatagaa gcagcaatag atttagctaa agaggatatt tctcctaagt caatagaaat
     3061 aaatttatta ggatgtaata tgtttagcta ctctatcaac gtagaggaga cttatcctgg
     3121 aaaattatta cttaaagtta aagataaaat atcagaatta atgccatcta taagtcaaga
     3181 ctctattata gtaagtgcaa atcaatatga agttagaata aatagtgaag gaagaagaga
     3241 attattggat cattctggtg aatggataaa taaagaagaa agtattataa aggatatttc
     3301 atcaaaagaa tatatatcat ttaatcctaa agaaaataaa attacagtaa aatctaaaaa
     3361 tttacctgag ctatctacat tattacaaga aattagaaat aattctaatt caagtgatat
     3421 tgaactagaa gaaaaagtaa tgttaacaga atgtgagata aatgttattt caaatataga
     3481 tacgcaaatt gttgaggaaa ggattgaaga agctaagaat ttaacttctg actctattaa
     3541 ttatataaaa gatgaattta aactaataga atctatttct gatgcactat gtgacttaaa
     3601 acaacagaat gaattagaag attctcattt tatatctttt gaggacatat cagagactga
     3661 tgagggattt agtataagat ttattaataa agaaactgga gaatctatat ttgtagaaac
     3721 tgaaaaaaca atattctctg aatatgctaa tcatataact gaagagattt ctaagataaa
     3781 aggtactata tttgatactg taaatggtaa gttagtaaaa aaagtaaatt tagatactac
     3841 acacgaagta aatactttaa atgctgcatt ttttatacaa tcattaatag aatataatag
     3901 ttctaaagaa tctcttagta atttaagtgt agcaatgaaa gtccaagttt acgctcaatt
     3961 atttagtact ggtttaaata ctattacaga tgcagccaaa gttgttgaat tagtatcaac
     4021 tgcattagat gaaactatag acttacttcc tacattatct gaaggattac ctataattgc
     4081 aactattata gatggtgtaa gtttaggtgc agcaatcaaa gagctaagtg aaacgagtga
     4141 cccattatta agacaagaaa tagaagctaa gataggtata atggcagtaa atttaacaac
     4201 agctacaact gcaatcatta cttcatcttt gggggtagct agtggattta gtatactttt
     4261 agttccttta gcaggaattt cagcaggtat accaagctta gtaaacaatg aacttgtact
     4321 tcgagataag gcaacaaagg ttgtagatta ttttaaacat gtttcattag ttgaaactga
     4381 aggagtattt actttattag atgataaaat aatgatgcca caagatgatt tagtgatatc
     4441 agaaatagat tttaataata attcaatagt tttaggtaaa tgtgaaatct ggagaatgga
     4501 aggtggttca ggtcatactg taactgatga tatagatcac ttcttttcag caccatcaat
     4561 aacatataga gagccacact tatctatata tgacgtattg gaagtacaaa aagaagaact
     4621 tgatttgtca aaagatttaa tggtattacc taatgctcca aatagagtat ttgcttggga
     4681 aacaggatgg acaccaggtt taagaagctt agaaaatgat ggcacaaaac tgttagaccg
     4741 tataagagat aactatgaag gtgagtttta ttggagatat tttgctttta tagctgatgc
     4801 tttaataaca acattaaaac caagatatga agatactaat ataagaataa atttagatag
     4861 taatactaga agttttatag ttccaataat aactacagaa tatataagag aaaaattatc
     4921 atattctttc tatggttcag gaggaactta tgcattgtct ctttctcaat ataatatggg
     4981 tataaatata gaattaagtg aaagtgatgt ttggattata gatgttgata atgttgtgag
     5041 agatgtaact atagaatctg ataaaattaa aaaaggtgat ttaatagaag gtattttatc
     5101 tacactaagt attgaagaga ataaaattat cttaaatagc catgagatta atttttctgg
     5161 tgaggtaaat ggaagtaatg gatttgtttc tttaacattt tcaattttag aaggaataaa
     5221 tgcaattata gaagttgatt tattatctaa atcatataaa ttacttattt ctggcgaatt
     5281 aaaaatattg atgttaaatt caaatcatat tcaacagaaa atagattata taggattcaa
     5341 tagcgaatta cagaaaaata taccatatag ctttgtagat agtgaaggaa aagagaatgg
     5401 ttttattaat ggttcaacaa aagaaggttt atttgtatct gaattacctg atgtagttct
     5461 tataagtaag gtttatatgg atgatagtaa gccttcattt ggatattata gtaataattt
     5521 gaaagatgtc aaagttataa ctaaagataa tgttaatata ttaacaggtt attatcttaa
     5581 ggatgatata aaaatctctc tttctttgac tctacaagat gaaaaaacta taaagttaaa
     5641 tagtgtgcat ttagatgaaa gtggagtagc tgagattttg aagttcatga atagaaaagg
     5701 taatacaaat acttcagatt ctttaatgag ctttttagaa agtatgaata taaaaagtat
     5761 tttcgttaat ttcttacaat ctaatattaa gtttatatta gatgctaatt ttataataag
     5821 tggtactact tctattggcc aatttgagtt tatttgtgat gaaaatgata atatacaacc
     5881 atatttcatt aagtttaata cactagaaac taattatact ttatatgtag gaaatagaca
     5941 aaatatgata gtggaaccaa attatgattt agatgattct ggagatatat cttcaactgt
     6001 tatcaatttc tctcaaaagt atctttatgg aatagacagt tgtgttaata aagttgtaat
     6061 ttcaccaaat atttatacag atgaaataaa tataacgcct gtatatgaaa caaataatac
     6121 ttatccagaa gttattgtat tagatgcaaa ttatataaat gaaaaaataa atgttaatat
     6181 caatgatcta tctatacgat atgtatggag taatgatggt aatgatttta ttcttatgtc
     6241 aactagtgaa gaaaataagg tgtcacaagt taaaataaga ttcgttaatg tttttaaaga
     6301 taagactttg gcaaataagc tatcttttaa ctttagtgat aaacaagatg tacctgtaag
     6361 tgaaataatc ttatcattta caccttcata ttatgaggat ggattgattg gctatgattt
     6421 gggtctagtt tctttatata atgagaaatt ttatattaat aactttggaa tgatggtatc
     6481 tggattaata tatattaatg attcattata ttattttaaa ccaccagtaa ataatttgat
     6541 aactggattt gtgactgtag gcgatgataa atactacttt aatccaatta atggtggagc
     6601 tgcttcaatt ggagagacaa taattgatga caaaaattat tatttcaacc aaagtggagt
     6661 gttacaaaca ggtgtattta gtacagaaga tggatttaaa tattttgccc cagctaatac
     6721 acttgatgaa aacctagaag gagaagcaat tgattttact ggaaaattaa ttattgacga
     6781 aaatatttat tattttgatg ataattatag aggagctgta gaatggaaag aattagatgg
     6841 tgaaatgcac tattttagcc cagaaacagg taaagctttt aaaggtctaa atcaaatagg
     6901 tgattataaa tactatttca attctgatgg agttatgcaa aaaggatttg ttagtataaa
     6961 tgataataaa cactattttg atgattctgg tgttatgaaa gtaggttaca ctgaaataga
     7021 tggcaagcat ttctactttg ctgaaaacgg agaaatgcaa ataggagtat ttaatacaga
     7081 agatggattt aaatattttg ctcatcataa tgaagattta ggaaatgaag aaggtgaaga
     7141 aatctcatat tctggtatat taaatttcaa taataaaatt tactattttg atgattcatt
     7201 tacagctgta gttggatgga aagatttaga ggatggttca aagtattatt ttgatgaaga
     7261 tacagcagaa gcatatatag gtttgtcatt aataaatgat ggtcaatatt attttaatga
     7321 tgatggaatt atgcaagttg gatttgtcac tataaatgat aaagtcttct acttctctga
     7381 ctctggaatt atagaatctg gagtacaaaa catagatgac aattatttct atatagatga
     7441 taatggtata gttcaaattg gtgtatttga tacttcagat ggatataaat attttgcacc
     7501 tgctaatact gtaaatgata atatttacgg acaagcagtt gaatatagtg gtttagttag
     7561 agttggggaa gatgtatatt attttggaga aacatataca attgagactg gatggatata
     7621 tgatatggaa aatgaaagtg ataaatatta tttcaatcca gaaactaaaa aagcatgcaa
     7681 aggtattaat ttaattgatg atataaaata ttattttgat gagaagggca taatgagaac
     7741 gggtcttata tcatttgaaa ataataatta ttactttaat gagaatggtg aaatgcaatt
     7801 tggttatata aatatagaag ataagatgtt ctattttggt gaagatggtg tcatgcagat
     7861 tggagtattt aatacaccag atggatttaa atactttgca catcaaaata ctttggatga
     7921 gaattttgag ggagaatcaa taaactatac tggttggtta gatttagatg aaaagagata
     7981 ttattttaca gatgaatata ttgcagcaac tggttcagtt attattgatg gtgaggagta
     8041 ttattttgat cctgatacag ctcaattagt gattagtgaa tagataaaaa tatgttaaat
     8101 atatcctctt atacttaaat atataaaaat aaacaaaatg atacactaca taaagtgttc
     8161 tatctaatat gaagatttac caataaaaag gtggactatg atgaatgcac agtagttcac
     8221 ctttttatat ttctaatggt aacaaaatat ttttttatat aaacctagga ggcgttatga
     8281 atatgacaat atctttttta tcagagcata tatttataaa gttagtaatt ttaactatat
     8341 catttgatac attattagga tgtttaagtg caataaaaag tcgtaaattt aattctagtt
     8401 ttggaataga tggaggaatc agaaaagtag caatgatagc atgtatattt tttttatcag
     8461 tagttgacat tcttacaaag tttaactttt tatttatgtt accacaagat tgtatcaatt
     8521 ttttaagact aaaacatctt ggaatatctg aatttttctc tattttattt attttatatg
     8581 aaagtgtaag tatattaaaa aatatgtgct tatgtggatt accagtacct aagagattaa
     8641 aggaaaaaat agcaatttta ctagatgcaa tgacagatga aatgaatgct aaggatgaaa
     8701 agtaagtaat ggtagatata ataaagatat taacaaataa aaagtgttat ccaaataaga
     8761 atagctgaaa gttatcataa ttcatgaaac taataatgaa aacgagggag cagatgccaa
     8821 gagacacaca agtattaaat acatataatt tcgaagcaag tgttcattac tatatagatg
     8881 acaaggtagt atatcaaaca ttggttcaca aagatggtgc atggtcagtt ggtaaaatct
     8941 attaagctac attagttaca gatatcacaa actataatag ttaaacatag aaatatgtgt
     9001 aaattgtgat tgaaattatt caaaaacaca aaaatacgtg atgaaggaca aaatgatata
     9061 gaaaataagt atcaaacctt aataaatgat ttaattgata gtttaaaagt tataggaaaa
     9121 atatataaag aaataaaaac attaaaaaaa tataagatat gtttacaaat tactatcaga
     9181 caatctcctt atctaataga agagtcaatt aactaattga gtatctttaa attgaaatgt
     9241 taggaagtga tttaaatatg aaaacttaaa ttataaaaaa tcaatattaa tttattttta
     9301 aaaaatagaa aggagtgtat aagatttatt ttcaaagttt aaaaacaaga aaatcaattt
     9361 aaatttcaga aggaataaat gtggttatag aagtggattt attatcaaaa ataataatac
     9421 taggaggttt ttatgtcttt aatatctaaa gaagagttaa taaaactcgc atatagcatt
     9481 agaccaagag aaaatgagta taaaactata ctaactaatt tagacgaata taataagtta
     9541 actacaaaca ataatgaaaa taaatattta caattaaaaa aactaaatga atcaattgat
     9601 gtttttatga ataaatataa aacttcaagc agaaatagag cactctctaa tctaaaaaaa
     9661 gatatattaa aagaagtaat tcttattaaa aattccaata caagccctgt agaaaaaaat
     9721 ttacattttg tatggatagg tggagaagtc agtgatattg ctcttgaata cataaaacaa
     9781 tgggctgata ttaatgcaga atataatatt aaactgtggt atgatagtga agcattctta
     9841 gtaaatacac taaaaaaggc tatagttgaa tcttctacca ctgaagcatt acagctacta
     9901 gaggaagaga ttcaaaatcc tcaatttgat aatatgaaat tttacaaaaa aaggatggaa
     9961 tttatatatg atagacaaaa aaggtttata aattattata aatctcaaat caataaacct
    10021 acagtaccta caatagatga tattataaag tctcatctag tatctgaata taatagagat
    10081 gaaactgtat tagaatcata tagaacaaat tctttgagaa aaataaatag taatcatggg
    10141 atagatatca gggctaatag tttgtttaca gaacaagagt tattaaatat ttatagtcag
    10201 gagttgttaa atcgtggaaa tttagctgca gcatctgaca tagtaagatt attagcccta
    10261 aaaaattttg gcggagtata tttagatgtt gatatgcttc caggtattca ctctgattta
    10321 tttaaaacaa tatctagacc tagctctatt ggactagacc gttgggaaat gataaaatta
    10381 gaggctatta tgaagtataa aaaatatata aataattata catcagaaaa ctttgataaa
    10441 cttgatcaac aattaaaaga taattttaaa ctcattatag aaagtaaaag tgaaaaatct
    10501 gagatatttt ctaaattaga aaatttaaat gtatctgatc ttgaaattaa aatagctttc
    10561 gctttaggca gtgttataaa tcaagccttg atatcaaaac aaggttcata tcttactaac
    10621 ctagtaatag aacaagtaaa aaatagatat caatttttaa accaacacct taacccagcc
    10681 atagagtctg ataataactt cacagatact actaaaattt ttcatgattc attatttaat
    10741 tcagctaccg cagaaaactc tatgttttta acaaaaatag caccatactt acaagtaggt
    10801 tttatgccag aagctcgctc cacaataagt ttaagtggtc caggagctta tgcgtcagct
    10861 tactatgatt tcataaattt acaagaaaat actatagaaa aaactttaaa agcatcagat
    10921 ttaatagaat ttaaattccc agaaaataat ctatctcaat tgacagaaca agaaataaat
    10981 agtctatgga gctttgatca agcaagtgca aaatatcaat ttgagaaata tgtaagagat
    11041 tatactggtg gatctctttc tgaagacaat ggggtagact ttaataaaaa tactgccctc
    11101 gacaaaaact atttattaaa taataaaatt ccatcaaaca atgtagaaga agctggaagt
    11161 aaaaattatg ttcattatat catacagtta caaggagatg atataagtta tgaagcaaca
    11221 tgcaatttat tttctaaaaa tcctaaaaat agtattatta tacaacgaaa tatgaatgaa
    11281 agtgcaaaaa gctacttttt aagtgatgat ggagaatcta ttttagaatt aaataaatat
    11341 aggatacctg aaagattaaa aaataaggaa aaagtaaaag taacctttat tggacatggt
    11401 aaagatgaat tcaacacaag cgaatttgct agattaagtg tagattcact ttccaatgag
    11461 ataagttcat ttttagatac cataaaatta gatatatcac ctaaaaatgt agaagtaaac
    11521 ttacttggat gtaatatgtt tagttatgat tttaatgttg aagaaactta tcctgggaag
    11581 ttgctattaa gtattatgga caaaattact tccactttac ctgatgtaaa taaaaattct
    11641 attactatag gagcaaatca atatgaagta agaattaata gtgagggaag aaaagaactt
    11701 ctggctcact caggtaaatg gataaataaa gaagaagcta ttatgagcga tttatctagt
    11761 aaagaataca ttttttttga ttctatagat aataagctaa aagcaaagtc caagaatatt
    11821 ccaggattag catcaatatc agaagatata aaaacattat tacttgatgc aagtgttagt
    11881 cctgatacaa aatttatttt aaataatctt aagcttaata ttgaatcttc tattggtgat
    11941 tacatttatt atgaaaaatt agagcctgtt aaaaatataa ttcacaattc tatagatgat
    12001 ttaatagatg agttcaatct acttgaaaat gtatctgatg aattatatga attaaaaaaa
    12061 ttaaataatc tagatgagaa gtatttaata tcttttgaag atatctcaaa aaataattca
    12121 acttactctg taagatttat taacaaaagt aatggtgagt cagtttatgt agaaacagaa
    12181 aaagaaattt tttcaaaata tagcgaacat attacaaaag aaataagtac tataaagaat
    12241 agtataatta cagatgttaa tggtaattta ttggataata tacagttaga tcatacttct
    12301 caagttaata cattaaacgc agcattcttt attcaatcat taatagatta tagtagcaat
    12361 aaagatgtac tgaatgattt aagtacctca gttaaggttc aactttatgc tcaactattt
    12421 agtacaggtt taaatactat atatgactct atccaattag taaatttaat atcaaatgca
    12481 gtaaatgata ctataaatgt actacctaca ataacagagg ggatacctat tgtatctact
    12541 atattagacg gaataaactt aggtgcagca attaaggaat tactagacga acatgaccca
    12601 ttactaaaaa aagaattaga agctaaggtg ggtgttttag caataaatat gtcattatct
    12661 atagctgcaa ctgtagcttc aattgttgga ataggtgctg aagttactat tttcttatta
    12721 cctatagctg gtatatctgc aggaatacct tcattagtta ataatgaatt aatattgcat
    12781 gataaggcaa cttcagtggt aaactatttt aatcatttgt ctgaatctaa aaaatatggc
    12841 cctcttaaaa cagaagatga taaaatttta gttcctattg atgatttagt aatatcagaa
    12901 atagatttta ataataattc gataaaacta ggaacatgta atatattagc aatggagggg
    12961 ggatcaggac acacagtgac tggtaatata gatcactttt tctcatctcc atctataagt
    13021 tctcatattc cttcattatc aatttattct gcaataggta tagaaacaga aaatctagat
    13081 ttttcaaaaa aaataatgat gttacctaat gctccttcaa gagtgttttg gtgggaaact
    13141 ggagcagttc caggtttaag atcattggaa aatgacggaa ctagattact tgattcaata
    13201 agagatttat acccaggtaa attttactgg agattctatg cttttttcga ttatgcaata
    13261 actacattaa aaccagttta tgaagacact aatattaaaa ttaaactaga taaagatact
    13321 agaaacttca taatgccaac tataactact aacgaaatta gaaacaaatt atcttattca
    13381 tttgatggag caggaggaac ttactcttta ttattatctt catatccaat atcaacgaat
    13441 ataaatttat ctaaagatga tttatggata tttaatattg ataatgaagt aagagaaata
    13501 tctatagaaa atggtactat taaaaaagga aagttaataa aagatgtttt aagtaaaatt
    13561 gatataaata aaaataaact tattataggc aatcaaacaa tagatttttc aggcgatata
    13621 gataataaag atagatatat attcttgact tgtgagttag atgataaaat tagtttaata
    13681 atagaaataa atcttgttgc aaaatcttat agtttgttat tgtctgggga taaaaattat
    13741 ttgatatcca atttatctaa tactattgag aaaatcaata ctttaggcct agatagtaaa
    13801 aatatagcgt acaattacac tgatgaatct aataataaat attttggagc tatatctaaa
    13861 acaagtcaaa aaagcataat acattataaa aaagacagta aaaatatatt agaattttat
    13921 aatgacagta cattagaatt taacagtaaa gattttattg ctgaagatat aaatgtattt
    13981 atgaaagatg atattaatac tataacagga aaatactatg ttgataataa tactgataaa
    14041 agtatagatt tctctatttc tttagttagt aaaaatcaag taaaagtaaa tggattatat
    14101 ttaaatgaat ccgtatactc atcttacctt gattttgtga aaaattcaga tggacaccat
    14161 aatacttcta attttatgaa tttatttttg gacaatataa gtttctggaa attgtttggg
    14221 tttgaaaata taaattttgt aatcgataaa tactttaccc ttgttggtaa aactaatctt
    14281 ggatatgtag aatttatttg tgacaataat aaaaatatag atatatattt tggtgaatgg
    14341 aaaacatcgt catctaaaag cactatattt agcggaaatg gtagaaatgt tgtagtagag
    14401 cctatatata atcctgatac gggtgaagat atatctactt cactagattt ttcctatgaa
    14461 cctctctatg gaatagatag atatatcaat aaagtattga tagcacctga tttatataca
    14521 agtttaataa atattaatac caattattat tcaaatgagt actaccctga gattatagtt
    14581 cttaacccaa atacattcca caaaaaagta aatataaatt tagatagttc ttcttttgag
    14641 tataaatggt ctacagaagg aagtgacttt attttagtta gatacttaga agaaagtaat
    14701 aaaaaaatat tacaaaaaat aagaatcaaa ggtatcttat ctaatactca atcatttaat
    14761 aaaatgagta tagattttaa agatattaaa aaactatcat taggatatat aatgagtaat
    14821 tttaaatcat ttaattctga aaatgaatta gatagagatc atttaggatt taaaataata
    14881 gataataaaa cttattacta tgatgaagat agtaaattag ttaaaggatt aatcaatata
    14941 aataattcat tattctattt tgatcctata gaatttaact tagtaactgg atggcaaact
    15001 atcaatggta aaaaatatta ttttgatata aatactggag cagctttaac tagttataaa
    15061 attattaatg gtaaacactt ttattttaat aatgatggtg tgatgcagtt gggagtattt
    15121 aaaggacctg atggatttga atattttgca cctgccaata ctcaaaataa taacatagaa
    15181 ggtcaggcta tagtttatca aagtaaattc ttaactttga atggcaaaaa atattatttt
    15241 gataatgact caaaagcagt cactggatgg agaattatta acaatgagaa atattacttt
    15301 aatcctaata atgctattgc tgcagtcgga ttgcaagtaa ttgacaataa taagtattat
    15361 ttcaatcctg acactgctat catctcaaaa ggttggcaga ctgttaatgg tagtagatac
    15421 tactttgata ctgataccgc tattgccttt aatggttata aaactattga tggtaaacac
    15481 ttttattttg atagtgattg tgtagtgaaa ataggtgtgt ttagtacctc taatggattt
    15541 gaatattttg cacctgctaa tacttataat aataacatag aaggtcaggc tatagtttat
    15601 caaagtaaat tcttaacttt gaatggtaaa aaatattact ttgataataa ctcaaaagca
    15661 gttaccggat ggcaaactat tgatagtaaa aaatattact ttaatactaa cactgctgaa
    15721 gcagctactg gatggcaaac tattgatggt aaaaaatatt actttaatac taacactgct
    15781 gaagcagcta ctggatggca aactattgat ggtaaaaaat attactttaa tactaacact
    15841 gctatagctt caactggtta tacaattatt aatggtaaac atttttattt taatactgat
    15901 ggtattatgc agataggagt gtttaaagga cctaatggat ttgaatattt tgcacctgct
    15961 aatacggatg ctaacaacat agaaggtcaa gctatacttt accaaaatga attcttaact
    16021 ttgaatggta aaaaatatta ctttggtagt gactcaaaag cagttactgg atggagaatt
    16081 attaacaata agaaatatta ctttaatcct aataatgcta ttgctgcaat tcatctatgc
    16141 actataaata atgacaagta ttactttagt tatgatggaa ttcttcaaaa tggatatatt
    16201 actattgaaa gaaataattt ctattttgat gctaataatg aatctaaaat ggtaacagga
    16261 gtatttaaag gacctaatgg atttgagtat tttgcacctg ctaatactca caataataac
    16321 atagaaggtc aggctatagt ttaccagaac aaattcttaa ctttgaatgg caaaaaatat
    16381 tattttgata atgactcaaa agcagttact ggatggcaaa ccattgatgg taaaaaatat
    16441 tactttaatc ttaacactgc tgaagcagct actggatggc aaactattga tggtaaaaaa
    16501 tattacttta atcttaacac tgctgaagca gctactggat ggcaaactat tgatggtaaa
    16561 aaatattact ttaatactaa cactttcata gcctcaactg gttatacaag tattaatggt
    16621 aaacattttt attttaatac tgatggtatt atgcagatag gagtgtttaa aggacctaat
    16681 ggatttgaat actttgcacc tgctaatact cataataata acatagaagg tcaagctata
    16741 ctttaccaaa ataaattctt aactttgaat ggtaaaaaat attactttgg tagtgactca
    16801 aaagcagtta ccggattgcg aactattgat ggtaaaaaat attactttaa tactaacact
    16861 gctgttgcag ttactggatg gcaaactatt aatggtaaaa aatactactt taatactaac
    16921 acttctatag cttcaactgg ttatacaatt attagtggta aacattttta ttttaatact
    16981 gatggtatta tgcagatagg agtgtttaaa ggacctgatg gatttgaata ctttgcacct
    17041 gctaatacag atgctaacaa tatagaaggt caagctatac gttatcaaaa tagattccta
    17101 tatttacatg acaatatata ttattttggt aataattcaa aagcggctac tggttgggta
    17161 actattgatg gtaatagata ttacttcgag cctaatacag ctatgggtgc gaatggttat
    17221 aaaactattg ataataaaaa tttttacttt agaaatggtt tacctcagat aggagtgttt
    17281 aaagggtcta atggatttga atactttgca cctgctaata cggatgctaa caatatagaa
    17341 ggtcaagcta tacgttatca aaatagattc ctacatttac ttggaaaaat atattacttt
    17401 ggtaataatt caaaagcagt tactggatgg caaactatta atggtaaagt atattacttt
    17461 atgcctgata ctgctatggc tgcagctggt ggacttttcg agattgatgg tgttatatat
    17521 ttctttggtg ttgatggagt aaaagcccct gggatatatg gctaaaatat atgtttgata
    17581 aaaaattatt cctgtgctac taagaaatta tttttatata ataaatattg agatttaatt
    17641 aaaagtcatg tgttattgta atacatgact tttagttaaa atttttctat catttaataa
    17701 tctattattc ttgactattt tataataaaa ttcatatatg gaaatattaa tactaaataa
    17761 ttaatagttg ataaaaaata gataatatgc taaaagcaaa aactaattta gagccttgta
    17821 actgtttatt tgcaattata aaaacatctt taaacatatt gactataata taaaatatta
    17881 actataatac aaaacaatat taattaattt tctctacagc tatccctggt atagttatat
    17941 ttccacccat agttgattca taagttatag ttccagcact tatacctctt atagttatgt
    18001 aatcatcctc taatactctt gaaggagtta ttgatttttt ataactacat agtacaacct
    18061 tatcataatt tccagacaca gctaatctta tttgcacctc atcaccatct tcaataactt
    18121 gaataacctt accttcaaat tttacctttt tgtacttata atcatcaggt gttctagcta
    18181 attggtcata agtaatacca gtatcatatc ctttcttctc ctcttcttct tttttgcgtt
    18241 gttcttcagc ctttttagct tcttcagctt ttttagcttc ttcagcttta cgttgatttt
    18301 cagcttcaat agctttcttt tcgtcgtctt tcattttgaa ccatggttca gcatcagaca
    18361 atttgctatt taaagtttct atttctgttt tttgagaatc tataactgat gtttggttag
    18421 aaatgacctc ctcatggtct tcagaacaag ctggtgagga tatattgcca aaaaacacgc
    18481 ctaaaataaa tgccagtaga gcaataccct ttgtgctctt aaagaattta attattttct
    18541 tagagcttcc ttttccttca ttactaaatt cgttaccctc atttttttta gaaaacataa
    18601 tacaatctcc cttttattag atatatttta ttttaaaagt taaaaactta aaataactag
    18661 ttgactaaaa attatatatt catattttcc taaaatatat ttcccctaag cataatttta
    18721 caaaataaag taagaagatt caatatattt tgaaaaaata agataaaaat gtcagattat
    18781 aatataattt tcttgaaggt gaattttata tattaaatta aaca
//
LOCUS       DAMJYR010000064        18709 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.064, whole genome shotgun sequence.
ACCESSION   DAMJYR010000064 DAMJYR010000000
VERSION     DAMJYR010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 18709)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18709)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18709
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.064"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            596..1969
                     /locus_tag="PC471_002263"
     CDS             596..1969
                     /locus_tag="PC471_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDF2789391.1"
                     /translation="MNEILENIDEGVHFVNKDGKTIIYNNSVQKMEKMNRHDILTNSF
                     FDIVNKMKINRSTLLEVLEKREVIKDNIQKYLDKNGREITAINTTIPIDIKGEFVGAL
                     EISKNMTTIENLLNRNYKKNLVNKVSIRQKKGYEFDDILGESNLIKEAIAKSKKACKS
                     DASVFIYGETGTGKELFSQSIHYGSNRKNYPFIAQNCAALPESLFEGILFGTSKGGFT
                     GAIDRPGLFEQANKGTLLLDEINSMPIMLQAKLLRVLQEGYVRRVGGTKDIPIDVRII
                     ATTNESPKVILNEHKMRKDLYYRLNIIHIKIPPLRDRKEDIPLLCKKFISKYNKKLNK
                     KVVGISDEAIKILKNYDWQGNIRELENIIYCTMSMMEDEVQIETSMINLNDYYNLKEN
                     KESNNYSLNDLEDKTLSKIISEIEEKYIYESLEKASYNITKAASILGMNRQNLQYKIK
                     KYNIKIL"
     gene            2225..3292
                     /locus_tag="PC471_002264"
     CDS             2225..3292
                     /locus_tag="PC471_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-binding protein"
                     /protein_id="HDF2789392.1"
                     /translation="MRKVRVGIWGFGAMGIGMANMILKKEGIEIVSVCSRSTSGKSMY
                     NVLGIERGERPEVIINKNYEEVFREKSVDVVLLATDSFTKKAFDKIIFLLNRKINVIS
                     TAEQMAYPQADDADLAKKMDEVAKENGVSILGTGINPGFVLDLLVLALSGTCEEVTSI
                     KAKRVNDLSPFGKSVMEEQGVGVTREEFIKGVEDKTIAGHVGFVESINMIADGLGWKL
                     DKIEQTKEPIMTTVDRKSKYGEALAGNVAGCRQCGYGYVNGEVLIEMEHPQQIIPEAE
                     GIKTGDYVSIKGIPNIDLQINPEIPGGVGTYAMIVNSIPLIINARPGLKTMLDIPVPR
                     AIMGDIRNQIEVELEEESKAN"
     gene            3436..3744
                     /locus_tag="PC471_002265"
     CDS             3436..3744
                     /locus_tag="PC471_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-ketopentanoate thiolase"
                     /protein_id="HDF2789393.1"
                     /translation="MDAKINDWVIIHNIVLTPEERAPQVPEDTKKVSLEMWVKGFIQS
                     DASIGDLVEVKTITGRLVKGNLLKINPYYTHDYGKCIPELLQIGIQAKEILFGGVYNE
                     "
     gene            3737..5167
                     /locus_tag="PC471_002266"
     CDS             3737..5167
                     /locus_tag="PC471_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent lyase/thiolase"
                     /protein_id="HDF2789394.1"
                     /translation="MNNSTSMKDMSYQAVMGRNNEIMKNAIGLDYSYFEQEGIGFDYE
                     KMMSETGYTLQDIEAIQSQYAVGNTPLIELKNLTKLARKCAKEGKGARIFVKDEAMNA
                     SGSFKARRAATAVYHAKQMGYKGVIAATSGNYGAAVASQAAMQGLKCIIVQECYDSNG
                     VGQPEIIEKARKCEAYGAEVVQLTVGPELFYTFLVLLEETGYFNASLYSPFGIAGVET
                     LGYELAIQFREKYKKDPDIVVCTNAGGGNLTGTARGLIKAGSINTKVVGASVDLKGLH
                     MASDNQFNKKSFTTGHTGFGIPYCTWPDRSDVPRSAARPLRYMDRYVLVKQGEVFYTT
                     ELLAQLEGIERGPAGNTSLAAAFSLAQELDEDKVIVVQETEYTGAGKHICPQLTFARE
                     NGIDIKFGNPREEIAGVNLILPERPELLKCVDIDMNKIRKSFIKNCISNNHIDNIDKL
                     SNRDIEFLMEEVKSSRDFVVDVLNNL"
     gene            5245..5619
                     /locus_tag="PC471_002267"
     CDS             5245..5619
                     /locus_tag="PC471_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine aminomutase subunit alpha"
                     /protein_id="HDF2789395.1"
                     /translation="MKKREDDFEVRRKHLQELSDDELKERFWSLATQIVEPMIELGKK
                     NTTPSIERSVLLRMGFSSLEVKPILEGVMERGLIGKGAGHVVYKLAKSKNITVREAGL
                     LLVKGEYWDEVTCLFREGVESC"
     gene            5613..7817
                     /locus_tag="PC471_002268"
     CDS             5613..7817
                     /locus_tag="PC471_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892628.1"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HDF2789396.1"
                     /translation="MLKENKKLDIDYILKDLDKYKPKRRGWVWREHLENLEMGPFKYK
                     DCTKPLKKSVGLPSSKYFNNIDPQPSPVITTEIASGRFEDDIRRMRMAAHHGADHLMV
                     IRTAGQSHFDGLIEGTPQGIGGVPITRKQVRAQRKALDFIEEEVGRPINYHSYVSGVA
                     GPEVAVMFAEEGVNGAHQDPQYNVLYRNINMVRSFVDACEAKKIMAFANIAQIDGAHN
                     ANATAREAWKVMPELMVQHALNSIFSEKIGIDKSNICLSTVPPTAPPAPCLKIDLPYA
                     VALRELFSDYKMRAQMNTKYMESSTREATVTHVLNLLTSVLTRADIQSTITPDEGRNV
                     PWHIYNIEACDTAKQALVGMDGLMDMIELKDVGELRDKARELKERAVLYMEEISEVGG
                     YFESVEQGFFVDSGNYPERNGDGISRKIKGGVGEGTVYEREEDYLAPVTAHFGYNNVA
                     QYDENAIDNPSMLIDGCTFENPDKIIYIDELDDFDNVENRLKESYEYRNGTKIKPEME
                     WCADGIVMITMMLPTDKRTAEFAALEFVKKMNLQEIEVISREVMHESEGTRIEVKGRV
                     PFDIDLNNLVIPEEPKVLTDEEIRADIEEKPMKIVSATVGEDEHSVGLREIIDIKHGG
                     IEKYGIECHYLGTSVPVEKLVDAAIELNADAILASTIISHDDIHYKNMKKLHDYCVEK
                     GIRDKVMIACGGTQVTPEIAVEQGIDAGFGRNSKGIHVATFLVEKRREMSNK"
     gene            7817..9226
                     /locus_tag="PC471_002269"
     CDS             7817..9226
                     /locus_tag="PC471_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate mutase L"
                     /protein_id="HDF2789397.1"
                     /translation="MENSKVSLKIDVLVAEIGSTTTVVNAFHDINTNNPIFLGQGQAP
                     TTVFEGGDVRNGLSGAIKDLANKLSVDDIEYNDMFATSSAAGGLKMTVHGLVYDMTVK
                     AAKEAALGAGAVIRQITSGRIKRTDLNKIKEINPNIILIAGGVDYGERDTAIYNAEMI
                     ASMNLGIPVIYAGNVENQEEIRLIFEDTNYKLYITENVYPKIDLLNIEPTRRIIQSVF
                     EEHITTAPGMKYIKEMVNENITPTPGAVMEASKLLYKNIGDLLTLDVGGATTDVHSVT
                     DGSDYINKILVNPEPTAKRSVEGDLGVYVNMKNIVEVIGKENLQSELSIDIDAVIENY
                     PPIPKSKEEILFVERLTKEAVVKAMLRHSGKIRNIYGTTGKVKIAEGKDLTEVRYIVG
                     TGGALTRLPSRIEILDNMLKYNKNNELLFPKEKTKILIDNDYIMASMGVLSKKYEEAS
                     LKLLLKSLNFEEERLCILG"
     gene            9211..10275
                     /locus_tag="PC471_002270"
     CDS             9211..10275
                     /locus_tag="PC471_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/ornithine racemase family PLP-dependent
                     enzyme"
                     /protein_id="HDF2789398.1"
                     /translation="MYPRLEIDIEKLKHNAKLISQMCHERNIKISFVTKSFCAQKEIV
                     EEICSEGIDHIADSRIQNLKNLQDINLPKILIRIPMLSELEEVINYCDISFNSELGTI
                     KKLNELCESKNIIHKIVLMFDLGDLREGYFYEEDFFSNVREIVRLKNIEIIGIATNLT
                     CYGAIIPSRENIGRLVSIAKRMEEKFGINLEIVSGGNSSSIHLLINNNMPEGITNLRI
                     GESILLGRETAYGENINGTYQDAFKLICQVVECKEKPSVPIGEIGVDAFRNKPVYEDK
                     GILKRAIIAIGKQDINIDSLIPIDTDIKILGASSDHMILDVSNTKYDYKLGDNLEFLL
                     TYGGIMSSSTSKYVSKKIIV"
     gene            10305..11726
                     /gene="nhaC"
                     /locus_tag="PC471_002271"
     CDS             10305..11726
                     /gene="nhaC"
                     /locus_tag="PC471_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HDF2789399.1"
                     /translation="MKERECRKASLFDAMIPITCLILFLSLGVLVYGSSPHVPLIGAA
                     AVAGLVAVYRLGFKWKELELSMFNSIKMAMQAILIIIIIGVLIGTWIVSGVVPCMIYW
                     GLKILSPNIFLVASTLVCAIVSLATGSSWSTMGTVGVALLGIGQSLGMPIGLIVGSII
                     SGAYFGDKLSPLSETTNLAPAMAGTDLFTHIKYMLYSTIPSLLICLVIYGVIGMKYSG
                     QALDIKQIELIRSTLDSTFNTLSPVLLLAPAIVIGLVVFKVPAIPGLIVGAVLGVLFA
                     VVFQGESMNTILDAAQNGYVSSTQIQEVDALLSKGGIMNMMSTVSLTICALSLGGILE
                     KTGMLEVVASSLLRLAKGVFGTVLCTMVTCTITNIIAGEQYLSIVIPGRMYNKEYKKR
                     GIHPKMLSRALEDSGTLTSPLVPWNTCGAYITATLGVSALTYGPYALLNIINPIVSLV
                     LIACKFKIARIEDEPETCLNFDV"
     gene            12155..12619
                     /locus_tag="PC471_002272"
     CDS             12155..12619
                     /locus_tag="PC471_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HDF2789400.1"
                     /translation="MTRISNITIAEQSEYCFLAIRKTIDFMVEFSEFSKQSFEKISKY
                     LEERGILSSGAPIVCFHNMDLAKLDVEVGFPVATYIDGKNEILQRIVPAQKIITAIDL
                     GPYELQDPTLEDLFSWANSNGYKLHGDIYYQYLNDINRPASEYLTKMMLPII"
     gene            12853..13215
                     /locus_tag="PC471_002273"
     CDS             12853..13215
                     /locus_tag="PC471_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDF2789401.1"
                     /translation="MKIYDILTRFYIQDIEKAIPFYENLLKEKCSLRFSYKEVGLELA
                     QIGNVLLLSGSDDALKPFIETKSTFMVDSVDEWRSYLLDNGAVVVRDKKKVPTGYNMT
                     LRHPDGTIIEYVQHTKQD"
     gene            13810..14670
                     /locus_tag="PC471_002274"
     CDS             13810..14670
                     /locus_tag="PC471_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2789402.1"
                     /translation="MSYKVINVITAIDYIEEHLSEKLDLDIVANAVHYSKYHLHRTFT
                     TSVGLTMHDYIKRRKLTEAAKLLVFSKKPIIEIALIAGYESQQAFTSIFKAMYKKSPN
                     KYRKEQEFYPLQLRFVLKKDNLFSKNVLELEKEIKLASMSDIPLWMNLVRLVIDGFPN
                     LQEEEYIYQLEQYILEERALILKLNNVAIANMVFNRETRSIDFFGIHPQYRNSDIAQV
                     FLKKVIEDFLIDTDISITTFREGDKADTGHRDMIKKLGFAEAELLVEFGYPTQKFILP
                     CVEERDNRQN"
     gene            14928..17042
                     /locus_tag="PC471_002275"
     CDS             14928..17042
                     /locus_tag="PC471_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding domain-containing protein"
                     /protein_id="HDF2789403.1"
                     /translation="MSENKQVVNCDIIASNSKHNMCEYDAELLTFFPDEIAHLIEINN
                     KLDNAFKKAENLVDKLDKDYMDAKMYMVKNRGEIDPHEMFQNEQGLKQIDNYGAFMVK
                     VRDKINKIKDSPYFARIDFRLKDMDDESKYYIGRFAFDYEDELIILDWRSPIASMFYD
                     YEIGKAGYDAPIGWVDGEITRKRQFKIKNGKLEYALESSINIQDDILQKELSHTSDEK
                     MKSIISTIQKEQNQIIRNDKADTLIIQGVAGSGKTSIALHRIAFLLYRFKDKISANNV
                     IILSPNKVFGDYISNVLPELGEEPLCELSFENIAEVQLDRVINFESEKDPLEINDAKW
                     SERVRFKSTLDFVKLIDDYIKQMPNKIFIPKDYTFGSFTAKSDWIQSRFEAYNRYPVK
                     KRLEKVAEDIHYKFESDNIMEEDLPKVKSILKSLNGMLTIKNTLTLYKDFFKQMNISN
                     MFVMATKKTLEWSDVYPFIYIHAAYEGIQEDKIIRHVVIDEMQDYTPIQYAVINLLFK
                     CKKTILGDFGQLVNPNHTHTLDDMKQLYNDGELVMLNKSYRSTFEIINFAKKIQDISS
                     LEPIERHGEEPALLKCNNEQDEINKIKIEIEEFKKSDNATLGIILKTDNDAKAVYDAL
                     REKYSVHLISSESSSFTKGVSITSIKMSKGLEFDEVIVPSVNNKTYYSDYDRSLLYIA
                     CTRAMHKLKLTYTGELTQLIDM"
     gene            17583..18536
                     /gene="blaCDD"
                     /locus_tag="PC471_002276"
     CDS             17583..18536
                     /gene="blaCDD"
                     /locus_tag="PC471_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDD family class D beta-lactamase"
                     /protein_id="HDF2789404.1"
                     /translation="MKRKKNFIWIAILLVGVVMVMYYVGKKHNDINQKTDKNYIKSEL
                     NKSELNKSKIENNNKNKKEKINMVDYSDCFEGISGGAIFYNTKNKEYNIYNKELIETR
                     RSPCSTFKIVSTLIGLEKGVINSKESVMGYDGTEYPNKNWNKNLSLEEAFKESCVWYY
                     KKLIDKVDAKSVQNILDDLKYGNCDISEWEGDLKNGKGHLNGFWLESSLQISPKEQVQ
                     TMAKIFEGDTNFKKEHINILRDIMKIDVNDKNINVYGKTGTGFDEKNKRVDAWFVGML
                     EREGDTYYFAIKSDDSNKEITGPKVKEIAINIIKKYYSVRE"
BASE COUNT         7461 a         2014 c         3409 g         5825 t
ORIGIN      
        1 aaaaaaataa atcttgttag ccaaaaataa gatttattat aaaaaattta attttaattt
       61 tctatttttg atactataat atataatctt gataatttaa ttaaaaatct agattttaca
      121 taccaaactg gtttcttatt tcgctatctt tacttatgaa ctcatatatt cctataaagg
      181 ttattttaga aaaagttggt tatttaaaca ttaataccac aatttctata gattgtttgg
      241 tgaataatct ataattatat tatggactaa aaaatccaat gagctaattt attgtttttt
      301 tatatttgag agattacaac ttattagttt gtatatctct ttttttaata aaatcaataa
      361 tatataaaac tattggcata tttaatgaca attcaaatat tgtatataac cataatgaaa
      421 gatatataaa taaaatttta aatatactta aaaatatatt aatataatgt acataaaaaa
      481 tatcataaat atatgataag ataaatacaa tagcaacatt ttattaattt tttgggtttt
      541 tatatattta ggtactgggg tgatatagtt gaaccaagag atatttttta aaataatgaa
      601 tgaaatattg gaaaatattg atgaaggggt acattttgtt aataaagacg ggaaaactat
      661 aatctacaat aattctgtac aaaaaatgga aaaaatgaac agacacgata ttttaaccaa
      721 ttcatttttt gatatagtca acaaaatgaa aataaacagg agtacactat tagaagtttt
      781 ggaaaaaagg gaagttatca aagacaatat tcaaaaatat ctggataaaa atggaagaga
      841 gataacagca attaatacta caataccaat tgatattaaa ggcgagtttg taggggcact
      901 tgagatatca aagaatatga caacgataga aaatttacta aacagaaatt ataaaaaaaa
      961 tttagtcaat aaagttagta ttagacaaaa aaagggttat gaatttgatg atattttagg
     1021 ggaaagtaat ttgattaaag aagcaatagc taaatctaag aaagcttgca agtctgatgc
     1081 ttcagtattt atatacggtg aaactggaac gggtaaagag ttatttagcc agtccattca
     1141 ctatggaagt aataggaaaa actatccatt tatagcacaa aattgtgcag ctttgccgga
     1201 atcactattc gaaggtattc tttttggaac tagcaaggga ggatttacag gagcaataga
     1261 taggccaggc ttatttgaac aagctaacaa agggacattg ttattggatg agataaattc
     1321 catgcctatt atgttacagg ctaaactatt aagggtttta caagaaggtt atgtaagaag
     1381 ggttggagga actaaggata tacctataga tgtaagaatt attgcaacga caaatgaaag
     1441 tccaaaggta attttaaacg agcataaaat gcgaaaagat ttatattata ggcttaacat
     1501 aattcatata aaaattcctc ctcttagaga tagaaaagag gacatacctc tattgtgcaa
     1561 aaaatttatt tcaaagtata ataaaaagtt aaataaaaaa gtagtaggaa tttcagatga
     1621 agctattaaa attttaaaaa attacgattg gcaaggtaac ataagagagc tagaaaacat
     1681 aatttattgt actatgagta tgatggaaga tgaggtacaa atagaaacaa gtatgattaa
     1741 tctaaacgac tattacaatc ttaaagagaa taaagagagt aacaattatt ctttaaacga
     1801 tttggaggac aaaacactta gtaagattat cagtgagata gaagaaaagt atatatatga
     1861 atcattagaa aaggcatctt acaatataac taaagctgca tcaattttag gtatgaatag
     1921 gcaaaattta cagtataaaa taaaaaaata taatatcaaa atattataaa taagcaagat
     1981 aaaaaaacaa aatcaaagta aaataaaaaa gcaaaataga ggagcaaaat tattataaaa
     2041 gagcaaaata ttttgctctt ttttttattt ctcataagaa atattatttt ttatttatga
     2101 aaatacataa taaaataatt atagtttcaa cgtttcaata aatatttact ttaattttaa
     2161 agttggcacg atttatgctt taactaatta ttgagaatat ataaattttt aggagggtgt
     2221 atatatgaga aaagtaagag taggaatatg gggatttgga gctatgggaa taggtatggc
     2281 taatatgatt ctaaagaaag aaggtataga aatagtatct gtttgtagta gaagtacttc
     2341 aggaaaaagt atgtataatg ttttaggaat tgaaagagga gaaagaccag aagttattat
     2401 caataaaaat tatgaagaag tttttagaga aaaaagtgta gatgttgttt tgttagcaac
     2461 tgattcattt actaaaaagg cttttgataa aataattttc ttattaaata gaaaaataaa
     2521 tgttatatca acagcagaac aaatggcata tccacaagct gatgatgcag atttagctaa
     2581 aaaaatggat gaagttgcaa aggaaaatgg agtaagtata cttggaacag gaataaatcc
     2641 aggatttgta ttagatttac tagtattggc gctttcaggc acttgtgaag aagttactag
     2701 tataaaagct aagagagtaa atgatttatc tccatttgga aagtctgtta tggaggaaca
     2761 aggtgttgga gtaacaaggg aagaatttat aaaaggtgta gaagataaga ctatagcagg
     2821 tcatgtaggt tttgtagaat caataaatat gatagctgat ggattaggtt ggaaattaga
     2881 taagatagaa caaacaaaag agccaataat gactacagta gatagaaaat ctaagtacgg
     2941 agaggcattg gctggaaatg ttgctggttg tagacaatgt ggatatggat atgtaaatgg
     3001 agaagtttta attgaaatgg agcatccaca acagataata ccagaagcag aaggtattaa
     3061 aacaggagac tatgtatcaa taaaaggtat accaaacatt gatttacaaa taaatcctga
     3121 aatacctgga ggagtaggta cttatgcaat gatagtaaac tctattccat taataataaa
     3181 tgctagacca ggtcttaaaa ctatgttgga tataccagta cctagagcaa taatgggaga
     3241 tataagaaat cagatagaag ttgaattgga agaagaatca aaggctaatt agtaagataa
     3301 cagctaacta attagtaaga tagcaactag ctaattagta agataacagt tagctaattg
     3361 gtaaaataat gtttaattta aaataacagt aaattaatta gtaaggcaat agtcaattaa
     3421 taaggggatg aaaaaatgga tgctaaaatt aatgattggg ttattataca caatatagta
     3481 ttgactccag aagaaagagc tccacaagtt ccagaggata ctaagaaagt atccttagaa
     3541 atgtgggtta aaggttttat acaaagtgat gcttctatag gcgacttagt agaagttaaa
     3601 acaattacag gcagacttgt aaaaggtaat ttacttaaaa taaatccata ttatactcat
     3661 gattatggta aatgtatacc agagttactc caaataggaa tccaagctaa agaaatatta
     3721 tttggaggtg tatataatga ataattcaac atcaatgaaa gatatgagtt atcaagctgt
     3781 tatgggaaga aataacgaaa taatgaaaaa tgcaatcgga ttggattatt cttattttga
     3841 acaagaggga atagggtttg attatgagaa aatgatgagt gaaacaggat atacattaca
     3901 agatattgaa gcaattcaat cacagtatgc tgttggaaat actcctttaa tagagcttaa
     3961 aaatttgact aaattagcta gaaaatgtgc taaagaaggt aaaggagcta gaatatttgt
     4021 aaaagatgaa gcaatgaatg catctggtag ttttaaagca agaagagcag ctactgcagt
     4081 atatcatgcc aaacaaatgg gatataaagg tgtaatagca gctactagtg gtaactatgg
     4141 tgcagctgta gcatcacaag cggcaatgca gggattaaaa tgtataatag ttcaagaatg
     4201 ttatgattct aatggtgtag gtcaacctga aattatagaa aaagcacgaa aatgtgaggc
     4261 atatggagca gaagttgttc agttaactgt tggaccagag ttattttata cgttcttagt
     4321 tttattagaa gaaacaggct attttaacgc atcattatat tcaccatttg gaattgcagg
     4381 tgtagaaact ttaggttatg agttagctat ccaatttaga gaaaagtata aaaaagaccc
     4441 tgatatagtt gtttgtacaa atgcaggtgg tggtaatctt actggaactg caagaggatt
     4501 gataaaagct ggttctatta atactaaagt agtaggagca agtgtagatt taaaaggatt
     4561 acacatggct agtgataatc aatttaataa aaaatcattt acaacaggac atacaggttt
     4621 tgggatacca tactgtacat ggcctgatag gtcagatgta cctaggtctg cagctagacc
     4681 acttagatat atggatagat atgtattagt aaaacaagga gaagtttttt atacaactga
     4741 attattggca cagctagaag gtatagaaag aggtccagca ggaaatacat ctttagcagc
     4801 agcattctct ttagctcaag agttagatga ggataaagtt atagtggtac aagaaacaga
     4861 atatacaggc gcaggtaaac atatatgtcc acagcttact tttgcaagag aaaatggaat
     4921 agatataaaa tttggaaatc caagagaaga aattgcagga gtaaacttaa tattaccaga
     4981 aagaccagaa ttattgaaat gtgttgatat tgatatgaat aaaataagaa agtcatttat
     5041 aaaaaattgt atatctaata atcacattga taatattgat aagctaagta atcgagatat
     5101 agaattttta atggaagaag taaaaagttc aagagatttt gttgttgatg tattaaataa
     5161 tttatagtaa ttattgtttt ttaatttaat ataaaataaa aattcagaaa attcgattaa
     5221 atcaatacga atggaggaat gttcatgaaa aaaagagagg atgattttga agttagaaga
     5281 aaacatcttc aagaactgag tgatgatgag ttaaaagaaa ggttttggag tttagctacc
     5341 cagatagtag aaccaatgat agaacttggt aaaaagaata caacaccatc aatagaaagg
     5401 tctgtcttgt tgagaatggg attttcttct cttgaagtaa aacctatttt agaaggggtt
     5461 atggaaagag gtcttatagg taaaggagca gggcatgtag tttataaatt ggctaaatct
     5521 aaaaatataa cggttagaga agcaggtttg ttattagtta agggtgaata ttgggatgaa
     5581 gttacatgtt tatttagaga aggggtagaa tcatgttaaa ggaaaataaa aaattggata
     5641 tagactatat attaaaagat ttggataaat ataaaccaaa acgaagaggt tgggtttgga
     5701 gagaacactt ggaaaatctt gaaatgggac cttttaaata taaagactgt acaaaacctt
     5761 taaagaagag tgtagggcta ccatcatcta agtatttcaa taatatagac cctcaaccaa
     5821 gtccagttat tactactgaa atagcttctg gtagatttga ggatgatata agaagaatga
     5881 gaatggcagc tcatcatgga gctgaccatc taatggttat cagaactgct ggacaatcac
     5941 attttgatgg gcttatagaa ggaactccac aaggtatagg tggagttcca ataacaagaa
     6001 aacaagtaag agctcaaagg aaggctcttg attttataga agaagaagtt ggtagaccta
     6061 ttaattacca ttcatatgta agtggtgttg ctggtcctga agtagcagta atgtttgctg
     6121 aggaaggagt caatggagca catcaagacc cacaatataa tgttctgtat aggaatataa
     6181 acatggtaag gtcttttgta gatgcttgtg aggcaaagaa aataatggct tttgccaata
     6241 tagctcaaat tgatggagca cataatgcta atgctactgc tagagaagca tggaaggtaa
     6301 tgcctgaact tatggttcaa cacgctttaa actctatatt ttcagaaaaa ataggtatag
     6361 ataaaagtaa tatatgctta tcaacagttc caccaacagc acctccagca ccatgtttaa
     6421 aaatagactt accttacgct gttgcactta gagagttatt tagtgattat aagatgagag
     6481 ctcaaatgaa tactaaatat atggaatcat caactagaga agcaacagtt actcatgtat
     6541 taaacttatt aacatcagta ttaactagag ctgacataca atcaactata acacctgatg
     6601 aaggtagaaa tgtaccttgg catatataca atatagaagc atgtgataca gcaaagcaag
     6661 ctttagttgg tatggatggg cttatggata tgatagaact taaagatgtg ggtgaattaa
     6721 gagacaaagc aagagaactt aaagaaagag ctgtattata tatggaagaa atatctgaag
     6781 ttggtggata ttttgaatct gtggaacaag gattttttgt tgattctgga aattatccag
     6841 aaagaaatgg tgatggaata tctagaaaaa taaaaggtgg agttggtgaa ggtactgttt
     6901 atgaaagaga agaagattat ttagcacctg taactgctca ctttggatac aataatgtag
     6961 ctcaatatga tgaaaatgct atagataatc catcaatgtt aatagatgga tgtacatttg
     7021 aaaatccaga caaaataata tatattgatg agcttgacga ttttgataat gtagaaaata
     7081 gactaaaaga atcatatgag tacagaaatg gaactaaaat taagcctgaa atggaatggt
     7141 gtgcagatgg aattgtaatg ataacaatga tgcttcctac tgataaaaga acagcagagt
     7201 ttgcagcttt ggaatttgtc aaaaaaatga acttacaaga aattgaggtc ataagtagag
     7261 aagttatgca tgaatctgaa gggactagaa tagaagtgaa aggaagagtt ccgtttgata
     7321 tagatttaaa caatttagta ataccagaag aacctaaggt actaactgat gaagaaataa
     7381 gagcagatat agaagaaaaa cctatgaaaa tagtttcagc tacggttgga gaagatgaac
     7441 attcagttgg tttaagagaa ataatagata taaaacatgg aggtattgaa aaatatggca
     7501 tagaatgcca ctatctaggg acttctgtac ctgtagaaaa attggtagat gcagcgattg
     7561 aactgaatgc agatgcaata cttgcatcta caataataag tcatgatgat attcactata
     7621 aaaacatgaa aaaattacac gattattgtg tagaaaaagg tataagagat aaagtaatga
     7681 ttgcgtgtgg aggaacacaa gtaacacctg aaatagctgt tgaacaaggc atagatgctg
     7741 gatttggaag aaacagtaaa ggcatacatg tagccacttt cttagttgaa aaaagaagag
     7801 aaatgagtaa taaataatgg aaaatagcaa ggtttcttta aaaatagatg tgttggtagc
     7861 tgaaattgga agtactacta cagtggtaaa tgctttccac gatattaata ctaacaatcc
     7921 tatatttttg ggtcaaggtc aagctccaac aacagtgttt gaaggtggcg atgtaagaaa
     7981 tggcttatca ggtgctatta aagatttagc taataaatta tctgtagatg atatagagta
     8041 taatgatatg tttgcaactt ctagtgcagc tggtggatta aaaatgactg ttcatggatt
     8101 agtctatgat atgacagtaa aagcagctaa agaagcagct ttaggggctg gagctgttat
     8161 tcgtcaaata acatcaggta gaattaaaag aactgattta aataaaataa aagaaataaa
     8221 cccaaacata attttaatag ctggtggagt cgattatgga gaaagagata cagctattta
     8281 taatgcagaa atgatagctt ctatgaatct aggcatacca gttatatatg ctggtaatgt
     8341 tgaaaatcaa gaggaaataa ggttaatatt tgaagatact aattataagc tttatataac
     8401 tgaaaatgta taccctaaaa tagatttatt gaatatagaa cctacaagaa gaatcattca
     8461 aagtgtattt gaagaacaca taactacagc accaggaatg aaatacatta aagaaatggt
     8521 taatgaaaat attactccaa ccccaggagc tgtaatggaa gcttctaaac ttttatataa
     8581 aaatattgga gatttattaa ctctggatgt aggtggagca actactgatg tacattctgt
     8641 tacagatgga agtgactata ttaataaaat tttggttaat cctgaaccta ctgcaaagag
     8701 aagtgttgag ggggatttag gtgtatatgt aaatatgaag aatatagtag aagttatagg
     8761 taaagaaaac ttacaaagtg aactatctat agatattgat gcagtaatag agaattatcc
     8821 tccaatacca aaatcaaaag aagaaatatt atttgttgag aggttgacaa aagaagctgt
     8881 tgttaaagct atgctaagac attcaggaaa aataagaaat atctatggaa caactggaaa
     8941 agtcaaaatt gcagaaggga aagatttaac agaagtaaga tatatagttg gtacaggggg
     9001 agcacttact aggctcccta gtagaattga aatattagat aatatgctca aatacaataa
     9061 aaataatgaa ttactatttc caaaggaaaa aacaaagata ttaatagata atgactatat
     9121 tatggcatct atgggtgttt tatctaaaaa atatgaagaa gcatctctta agctcctgtt
     9181 aaaaagtttg aattttgagg aggaaagatt atgtatccta ggttagaaat agatatagaa
     9241 aagttaaagc ataatgcaaa attaatttcg cagatgtgtc atgaaagaaa tattaagata
     9301 tcttttgtta ctaaatcatt ttgtgcacaa aaagaaatag tagaagaaat atgtagtgaa
     9361 ggaatagacc atatagcaga ttcaagaata caaaacttaa aaaatttaca agatataaat
     9421 ctacctaaga tattgattag aatcccaatg ttaagtgaat tagaagaagt tatcaattat
     9481 tgcgatatta gttttaactc agaattagga acaataaaga aattaaatga attatgtgaa
     9541 agtaaaaata ttattcataa gatagttctt atgtttgact taggcgattt acgagaagga
     9601 tacttttatg aagaagactt ttttagtaat gtaagagaga tagtaagatt aaaaaatatt
     9661 gaaataattg gtatagctac aaacttaacc tgttatggag ctattatacc atctagggaa
     9721 aatataggca gactggtaag tatagcaaaa agaatggaag aaaaatttgg aataaatcta
     9781 gaaatagtat ctggaggtaa ttcaagttct attcatctgt tgataaataa taatatgcca
     9841 gaaggtatta ctaatttgag aataggcgag tccattctac ttggtagaga aacggcatac
     9901 ggtgaaaata taaatggaac atatcaagat gcatttaaat taatatgcca agtagttgaa
     9961 tgtaaagaaa aaccatcagt tcctattgga gaaataggag ttgatgcctt tagaaataaa
    10021 cctgtttatg aagataaggg aatcttaaaa agggcaatca tagctatagg taaacaagat
    10081 ataaatattg actctttaat tccaatagac actgatataa aaatattagg ggcaagttca
    10141 gaccacatga tactagatgt aagtaacaca aaatatgatt ataaattagg tgacaattta
    10201 gaatttttat taacttatgg gggtatcatg tcatctagta cgagcaaata tgtttcaaaa
    10261 aaaattatag tgtagtgttt aaataattag gaggaaacta ttttatgaaa gagcgagaat
    10321 gtagaaaagc aagtctgttt gatgcaatga taccaattac ttgtttgata ttatttttaa
    10381 gtctaggagt attagtgtat ggttcatcac ctcatgtacc actaatagga gcagcagcag
    10441 tagcaggact tgtggctgta tatagacttg gatttaaatg gaaagagtta gaattaagta
    10501 tgtttaatag tataaagatg gcaatgcaag caatcttaat cataattata ataggcgtac
    10561 ttataggaac ttggatagta agtggagtag ttccatgtat gatttactgg ggactaaaaa
    10621 ttttatcacc aaatattttt ttagtagcat caacattagt ctgtgcaata gtatctcttg
    10681 cgacaggttc atcttggagc acaatgggta cagtaggagt agctctttta ggcattggtc
    10741 aaagtttagg tatgccaatt ggattaatcg taggttctat aatatctgga gcttactttg
    10801 gagataaact atcaccttta tctgaaacga ctaacttagc tccagcaatg gcaggaacag
    10861 atttgtttac acatataaaa tatatgctat attcgactat accatcatta ttaatttgtt
    10921 tagtaattta tggtgtaatt ggaatgaagt attcaggtca agcactagat ataaaacaaa
    10981 tagaattaat taggtctaca ttagattcta catttaatac tttatcacca gtattattat
    11041 tagctccagc aatagtaatt ggattggttg tatttaaagt tcctgctata ccagggctta
    11101 tagtaggtgc tgtattggga gtattgtttg cagtagtttt tcaaggagaa tctatgaata
    11161 cgatattaga tgcagcacaa aatggatatg tttcgtcaac acaaatacag gaggtagatg
    11221 ctttattatc taaaggtggg attatgaata tgatgtccac tgtatcctta acaatatgcg
    11281 ctttatctct tggaggtatt ttagaaaaaa caggtatgct tgaagtagta gcatcttctt
    11341 tactaagatt agcaaagggt gtatttggaa cagtattatg tactatggta acttgtacaa
    11401 ttaccaatat aatagcaggt gaacaatatc tatctatagt tataccagga agaatgtata
    11461 ataaggaata taaaaagaga ggtatacatc ctaagatgtt gtcgagagct ttagaagata
    11521 gtggaacttt aacctctcca ttagtaccat ggaatacatg cggggcgtat ataacagcaa
    11581 cattaggtgt atctgcacta acatatggtc catacgcttt attaaatatt ataaatccaa
    11641 tagtatcttt agtattaatt gcatgtaagt ttaaaatagc aagaatagaa gatgaacctg
    11701 aaacttgttt aaattttgat gtttgataat agataactta tgtaagaacg taggaactag
    11761 gggggtagcc tgtggggaat tagtaagaca tattttagtg tgcgaaattc tataaatcag
    11821 taatcttgtg actttagcca tgggaggttc agattagtaa atttatagtt ttatattagt
    11881 ctatattttg atcttttaat tgtttgtata aattttttat agaaaaagat ttcttttaga
    11941 cttgcctaaa aattaggcaa gtcttttaag tatgttctga ttataagagc actgtgtgac
    12001 aagaatgatg agattatttt tgctatactt tcgatataag aaatggaagg aggttatgtt
    12061 ttatattcat atgaaataat tagttacaca gtaaattttc gtataatttg gtataaaaag
    12121 attagaaatg aaaataaagt aatggaggaa taaaatgact agaatatcca atataacaat
    12181 agctgaacaa tctgagtact gttttttggc aatacgaaaa acaattgact ttatggtaga
    12241 gttttcagag ttttcaaaac aaagttttga aaaaatttct aaatatctgg aggagagagg
    12301 aattttatct tcaggagcac cgattgtatg ttttcataac atggatttag caaaattgga
    12361 tgttgaagta gggtttcctg tagccactta tatagatggg aaaaatgaga ttctacaaag
    12421 gattgttcct gcacagaaaa taataactgc aattgattta ggaccttatg aattacaaga
    12481 ccctacttta gaagacttat tttcatgggc aaatagcaat ggatataaac ttcatggcga
    12541 tatatattac cagtatttaa atgatatcaa tcgtccagct agtgaatatc ttacgaaaat
    12601 gatgttaccc attatataga aagttaaaga ttatcgtttg ttagttgata attagaagaa
    12661 catttactgc tactacataa attttatcct aaataatatc aaggtgattt aatttatgta
    12721 ttctaatgta accccaatat attgttaagg tctacttaag atgctcaaaa ggctatgctt
    12781 aatttattgt tatataattt gtagagtggt ataatttaga ggtaatatta tatagatata
    12841 tagggggaaa atatgaaaat atatgatata ttaacacgtt tttatattca agatatagaa
    12901 aaagccatac ctttttatga aaatctttta aaagaaaaat gttctttgcg tttttcctat
    12961 aaagaagttg ggttggaatt agcgcaaata ggaaatgttt tactattatc tggttctgat
    13021 gatgctctta aaccatttat agaaacaaaa tctactttta tggttgattc tgttgatgaa
    13081 tggagaagtt atctattgga taatggagca gttgtagtaa gagataagaa aaaagttcct
    13141 acaggatata atatgacatt gagacatcca gatggtacaa ttatagaata tgttcaacat
    13201 acaaagcagg attagttaaa aacagatttt actactgagc aactaattag aatctattct
    13261 tagacgtaca atttattatt tttggtatta acagattata agtcaaatta taatatttca
    13321 aaatagaata gaaatttatt tacataaaag agagtgttta aaaatgaact ttttagttca
    13381 tgaagcactc ttttttgtat gaaatcaaat acatcaatat ataaaataaa ctgatgaaga
    13441 gcttaaatta aaataaaaag attcataaca ggaccccatt aaactttaag atagggtaag
    13501 tttgtaatca tattaaataa ttgtaaaaag ctgtttttag tcgtataata taaatattta
    13561 tcatttaatt aaggaatata aaaggaatct aataagaaga aaataataaa taacttttta
    13621 gatttactaa atgaaaagtt attgaaaatt aaaggattag agtctttaat cagaaagtaa
    13681 aaagtaatat attaaattta tgaattgaaa acttaaacaa caaatttgtt tataaaatag
    13741 caagattcat taaatataac taagtaataa ttgatataat gttgcatgga taaaaggagg
    13801 gaagctgcaa tgtcttacaa agtaattaat gtcataactg ctattgacta tatagaggag
    13861 catttatcag aaaagctgga tttagatata gttgctaatg ctgttcatta ctcaaaatat
    13921 cacctgcata gaactttcac aacttctgtt ggtctaacaa tgcatgatta cataaagcgg
    13981 agaaagttga cagaagcagc aaaattattg gtattttcaa agaaaccaat tattgaaatt
    14041 gcattgatag ctggatatga aagtcaacag gcattcactt ctatatttaa agctatgtat
    14101 aaaaaatctc caaataaata taggaaagaa caagaatttt atcctttgca gctaagattt
    14161 gttttgaaaa aagataacct attttcaaag aatgtactag agttggaaaa agaaattaaa
    14221 cttgcaagta tgtctgacat accattatgg atgaatttag tgagattagt catagatgga
    14281 tttccaaatt tgcaagagga agaatatata tatcaattag aacagtatat tttagaagaa
    14341 agagctttaa tattaaaact taataatgtc gctattgcaa atatggtgtt taatagagaa
    14401 actagaagta ttgatttttt tggaatacat ccacagtatc gtaattcaga cattgcacaa
    14461 gtgtttttga agaaagttat tgaagatttc ttgatagata cagatatttc aattactacc
    14521 tttcgagagg gtgacaaagc tgatacagga catagagata tgataaagaa gttgggcttt
    14581 gcagaagcag aattgcttgt ggaatttgga tatcctactc aaaaatttat tcttccttgt
    14641 gtagaagaaa gagataatag acaaaattaa ttgtagatta aatgaaaagt ttaccacata
    14701 ttttttgttt gaattatttt atatataaga gcatttctgc ttttaatgta ttttgcttgt
    14761 aagtgtactt atggtaataa atatagaaag atttagtttt ttaaaaaagg aactttaaag
    14821 ctaaaaagct aacatttaag ttaaatagca agaatgatta aaaactccaa aacacaatta
    14881 agtataatga ttcagtgtaa gtacttaatt aacaaaggag gatttaaatg tctgaaaata
    14941 aacaagtagt aaattgcgat attattgcat ctaattctaa acataatatg tgtgaatatg
    15001 atgcagagtt attaactttt tttccagatg agattgcaca tctaatagag ataaataaca
    15061 agctagacaa tgcttttaag aaagctgaaa atcttgttga caagcttgat aaggattata
    15121 tggacgctaa aatgtatatg gtaaaaaatc gtggtgaaat agacccacat gaaatgtttc
    15181 aaaatgaaca aggactaaaa caaatagata actatggggc ttttatggta aaagttcgag
    15241 ataagattaa taaaatcaaa gattctccat actttgctag aatagatttt aggctgaaag
    15301 atatggatga tgaatctaag tattatattg gtagatttgc atttgattat gaagatgagc
    15361 ttataatact tgactggcgt tctccaattg ccagtatgtt ttatgattat gaaatcggaa
    15421 aagctggata tgatgctcca ataggatggg tggatggaga aataacaaga aaacgacaat
    15481 ttaaaattaa aaatggaaaa ttagaatatg cactagaaag ttccattaat atacaagatg
    15541 atattcttca aaaagaactt agtcacactt cagatgaaaa aatgaagtca atcatttcaa
    15601 caatacaaaa ggaacaaaat caaattatta gaaatgataa agctgataca ctgattattc
    15661 aaggggtagc tggttctggg aaaacatcta tagcattaca cagaattgct tttcttcttt
    15721 atcgatttaa agataaaata tcagcaaata atgtaattat cctatcacct aataaagtct
    15781 ttggagacta tatttccaat gtccttcctg agcttggaga agagccatta tgtgaattaa
    15841 gttttgaaaa tatagcagag gtacaattag atagagttat taattttgaa agtgagaaag
    15901 acccattaga aataaatgat gctaaatggt ctgaaagagt gcgttttaaa tctacacttg
    15961 attttgtaaa acttattgat gattatataa aacaaatgcc aaataagatt tttattccaa
    16021 aagactacac ttttggaagt tttacagcta aaagtgactg gatacagagt agatttgagg
    16081 catataacag atatccagtt aagaagagat tagagaaggt agctgaagac attcattata
    16141 aatttgaatc agataatatt atggaagaag atttaccaaa ggtaaaaagt atacttaaaa
    16201 gtttgaatgg aatgttgaca ataaaaaata ctcttacact atataaggat ttctttaaac
    16261 aaatgaatat atcaaacatg tttgtgatgg caacaaaaaa aactcttgaa tggtcagatg
    16321 tatacccatt tatatatatt catgctgcat atgaaggtat acaagaagat aagattataa
    16381 gacatgttgt tattgatgaa atgcaggatt acacacctat tcaatatgct gtaataaact
    16441 tactttttaa gtgtaaaaag actattcttg gagattttgg acaattggtt aatcctaatc
    16501 acactcatac actagatgat atgaaacagc tatataatga tggggaacta gttatgttga
    16561 ataagagtta ccgttctact tttgagatta ttaatttcgc caaaaagatt caagacatat
    16621 cttcattaga gcctattgaa cgacatggtg aagaaccagc tttattaaaa tgtaataacg
    16681 agcaagatga aataaataag ataaaaatag aaattgaaga atttaaaaag agtgataatg
    16741 cgactcttgg tattatatta aagacagata atgacgctaa agctgtatat gatgcgttaa
    16801 gagaaaaata tagtgttcac ttaatatctt ctgaaagttc tagctttaca aaaggagtat
    16861 ccataacttc tattaaaatg tcaaaaggtt tagagtttga tgaagttatt gtaccttcag
    16921 taaataataa aacatattat agtgattatg accgcagttt gttatatatt gcttgtacac
    16981 gagctatgca taaacttaag ttgacatata ctggtgaatt gactcaactt atagatatgt
    17041 agattatata cttattatgt aagtgtttac ttatataaat ataacctgtc aataaaattc
    17101 attaataaat caataaaaac aatatttgta aaagtaaaag ctataaatta tatattaaat
    17161 tttataattt atagctttta taatactgct tatcttaatc ctcccaatag caaaataatt
    17221 ttaaacatgc catattttat aaatgtaata acaattatac aattaaaata actttcccaa
    17281 actaacttga catttaaaac ttactattgt aatatttaaa tattacaata gtaagtttta
    17341 aatgtggaat caattattat atcacaaatt aaaaggagga caggatgtta ttaatagtta
    17401 cttaattatc ttcgcaatag gtttaatcaa cataattgta ctaggtgaag aaaatataag
    17461 agatacagct attcatttag tcttgtttct tataagtaga acctagattg gagtatatta
    17521 caaataatgt ctactatttt aatagtaggt tataaattta aatatgggag atgatataaa
    17581 ttatgaaaag aaaaaagaat tttatttgga tagctatctt attggttggt gtagttatgg
    17641 taatgtatta tgtagggaaa aaacataacg atataaatca aaaaactgat aagaattata
    17701 taaaatctga attaaataag tctgaattaa ataaatccaa aattgaaaac aataataaaa
    17761 acaaaaaaga aaaaattaat atggttgact atagtgattg ttttgaaggt attagtggag
    17821 gagctatttt ttataatact aaaaataaag aatataatat atataacaaa gaattaattg
    17881 aaacaagaag gtcaccatgt tctactttta aaatagtttc aactttaatt ggtttagaaa
    17941 aaggggtaat aaactcaaaa gaatcagtta tgggatatga tggtacagag tatccaaata
    18001 aaaattggaa taaaaatctt agtttagaag aggcatttaa ggagtcttgt gtttggtact
    18061 acaaaaaatt gatagataaa gttgatgcta aatctgtcca aaacatttta gatgatttaa
    18121 agtatgggaa ttgcgatatt agcgaatggg aaggagattt aaaaaatgga aagggacatt
    18181 taaatggatt ttggttagaa tcttcattac aaatatctcc aaaagaacaa gtgcaaacaa
    18241 tggcaaaaat atttgaggga gatacaaact ttaaaaaaga gcatattaat attttaagag
    18301 atattatgaa aatagatgta aatgataaaa acataaatgt atatggaaaa actggaacag
    18361 gatttgatga aaaaaataaa cgtgtggatg catggtttgt tggtatgcta gagcgtgaag
    18421 gtgacaccta ttattttgct attaaatcag atgattccaa taaagaaatt acagggccaa
    18481 aggtaaagga aattgcaatt aatataatta agaaatatta tagtgtaagg gaataaactt
    18541 aattttattt gtaaatatta tttacctata gtctgtaata tttattaaat aattttacta
    18601 aattgagtct atatagagca aaactccttt agtacataaa atacttgcta ttgattatta
    18661 aattgaatac ctatagattt aaagttaata ccttttaaag actatgtaa
//
LOCUS       DAMJYR010000065        18662 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.065, whole genome shotgun sequence.
ACCESSION   DAMJYR010000065 DAMJYR010000000
VERSION     DAMJYR010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 18662)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18662)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18662
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.065"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(154..1515)
                     /gene="rlmD"
                     /locus_tag="PC471_002277"
     CDS             complement(154..1515)
                     /gene="rlmD"
                     /locus_tag="PC471_002277"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HDF2789405.1"
                     /translation="MSVKKKDVIEFEVDKMEFGGTSLSQVGDRVIHMKGGISGQKVRA
                     GVKKVRSKKAEVKMIELLENSPLETEETCKHFRECGGCTLLSVPYEKQLEIKEKQVMD
                     LFLKQDLFGFQFLGIEGSPENKYYRNKMEYTFGDEVKNGPLTLGLHKKGKHIDIQTVE
                     ECMLVDEDFSKILVSSVEFFNEKKLPYYRTMNHKGYLRHLVVRKGIHTNEIMVNIVTS
                     SQEDFDMYEFKDMLLGIDLKAELVGVLHTINDGLADAVQCDELRVLYGRDYIQEEILG
                     LKFKISPFSFFQTNTKGAEVLYSIARDFIGDYNDKVVFDLYSGTGSIGQVMAGAAKKV
                     YGIEIVEEAVVAANENAKLNGLTNCEFIAGDVAKVVKDLKDKPDLIIVDPPRPGIHKD
                     AIRDICGFGAKEIVYISCNPKSLVVDLVDFKGYGYEIKMVKCMDMFPNTPHCETVVLL
                     RRK"
     gene            complement(1542..2264)
                     /locus_tag="PC471_002278"
     CDS             complement(1542..2264)
                     /locus_tag="PC471_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HDF2789406.1"
                     /translation="MAKKQRIDKILSNLGYGSRSEIKKYCKQGSVVVNGSEVSNPGTQ
                     VDTENDEILFNGEEVIYREYIYLMMNKPDGYISATTDKYDPTVLDLIDLSYLAFEPFP
                     VGRLDKDTEGLLVLTNDGKLSHRVLSPKKHVPKTYYAKIDGVVTEEDVEAFLEGVVLD
                     DGYKTMPSQLNILKSDDESEIELTIHEGKFHQVKRMFESVGKKVVYLKRLSMGNLKLD
                     ESLELGEYRELTDEEVKLIEER"
     gene            complement(2264..2593)
                     /locus_tag="PC471_002279"
     CDS             complement(2264..2593)
                     /locus_tag="PC471_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1904 domain-containing protein"
                     /protein_id="HDF2789407.1"
                     /translation="MPQIKIRGINENDICKISEKMINDLVEAVKCPRDYFEIECIKSV
                     AIRDGKIADVYPFVEVAWFDRGQEVQDIVARIITDSIRNNLDVESIDLAFTVFEKEKY
                     YENGEHF"
     gene            2923..3849
                     /gene="fba"
                     /locus_tag="PC471_002280"
     CDS             2923..3849
                     /gene="fba"
                     /locus_tag="PC471_002280"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-1,6-bisphosphate aldolase"
                     /protein_id="HDF2789408.1"
                     /translation="MALITTKEMFKKAYEGGFAIGAFNISDLEQLQGVLKAAKAKNSY
                     VMIQASMSAVKYAGPHTLVEMVKAASDEIGVDVALHLDHGPNMDAIKTCIDAGFSSVM
                     IDGSHFDFEENVRITKEAVEYAHSKGVVVEAELGVLAGTEDDVTSDVHKYTQPAEAVE
                     FVERTGVDSLAIAIGTSHGAFKFKGEAKLRFDILEEIQSKLPGFPIVLHGASAVDQNA
                     VATCNEFGGNIAGAKGVPVDMLRKASSMAVCKINMDTDLRLAMTAAIRKFLAENPKEF
                     DPRKYIGAGRDAIQAVVESKIDDVLGSANSIN"
     gene            complement(3984..5615)
                     /locus_tag="PC471_002281"
     CDS             complement(3984..5615)
                     /locus_tag="PC471_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDF2789409.1"
                     /translation="MDNNLIGLIEHVENPAILCKESGEIIYCNHLIDSIFSFLDIKKP
                     RNINELDSNFDKTEILTDSKKKIAFRELRMTAHIYNMKDNNNENNIVYLFEKSLISDK
                     VIEDIIEHIDEVVVVFNKDGVIEKMNTVSDEILPFKRTEVLGRNITDLVRQGLVEEPI
                     ILNMLKVKKKIYRNIVYPDGKLIAYTAVPRWDSKGKLTGGVLTGRDISRVIKLESQIK
                     YSDISEDTEYISQSKIMDNIKKVVKRAAASDSSIFINGESGVGKEIIARTIYKYSSRR
                     DKPFIAINCGAIPNELLESEFFGYEEGSFTGAKKKGKKGLFEEANGGTIFLDEIGELP
                     MQMQKKLLRVIQENTITRIGGSKPIKIDVRYISATNISHEDLRNNLKFRQDLYYRLSV
                     IPVKIPPLRERKEDIVPLVNYFLKLYNEKYNREVEVSPKVIELLEEYSWPGNIRELKN
                     IIERFVVLSAKNVIGEDEFNMLINLDMIDNETDDLSPIVVNGIMNLNDAYKIVDQIMI
                     SKAINKYGSITKAAEVIGIYPSTIHRKIKSGHIHV"
     gene            complement(6098..7135)
                     /locus_tag="PC471_002282"
     CDS             complement(6098..7135)
                     /locus_tag="PC471_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HDF2789410.1"
                     /translation="MNDIKDLSSYKNVWIFAEQREGKIAPVVIELLGEGRKLAKEVDA
                     ELCAILLGKDVDGLAKELITFGADKVYVADDALLEKYTTDAYTKVIKDAIDEIKPEIM
                     LFGATHIGRDLAPRIASRVGTGLTADCTKLEIDPEDKKIKQTRPAFGGNIMATIICPN
                     HRPQMSTVRPGVMDKAEKDETRTGEVIALDYKITQDDIRTTVLETVKTKKDLVSLTDA
                     NVIVSGGLGLGGPEGFEMLKKLADKLGGVVGSSRAAVDAGWIDHSHQVGQTGTTVKPN
                     LYIACGISGAIQHLAGMQSSDFIIAINKNPAAPILEIADYGVVGDLHEIVPMLIEKLD
                     SVDDLLEAIKA"
     gene            complement(7154..7939)
                     /locus_tag="PC471_002283"
     CDS             complement(7154..7939)
                     /locus_tag="PC471_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012703997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HDF2789411.1"
                     /translation="MKILVCVKQVPDTNEVRINKETGTLIRDGVPSILNPDDANALEE
                     ALKIKDEHDDVTITVITMGPPQADFMLRECLAMGADDAILLSDRAFGGADTWATSNTI
                     AAGISKVGDYDIIFAGRQAIDGDTAQVGPQIAEKLDIPQVTYVQDFKIEGKDIIVQRQ
                     LEDGYELIKVSTPVLLTAVKELNTPRYMSVDKIVKAYKHDVKVWTIDDLDVNKEEVGL
                     KASPTKVFRSFTPEPRGKGEILEGKADEIASKIIIGLKQKHII"
     gene            complement(7972..9105)
                     /locus_tag="PC471_002284"
     CDS             complement(7972..9105)
                     /locus_tag="PC471_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003483287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HDF2789412.1"
                     /translation="MLYNKEQELLRKAVRDFVSKELDTLPAEMDKTGVMPKELIKKLA
                     DAKFISSNIPEEYGGGGAGYVSYAIVMEEIARRCASTATFVTAGSSLASLPILYNGTE
                     EQKQKYLKGIATGELIGAFGLTEPGAGSDAGGQQTTAELVGDHYILNGRKTFITNGPF
                     CDVAIVIAVTDRSKGLRGTSAFIVESKWDGFSTGAHEDKMGIRGTETSDLIFENVKVP
                     KENLLGKEGQGFKIAMGTLEVGRIGVAALALGIAQGALDEAVKYTKQRVQFGKPIAKF
                     QNTQFTIADMETKVCAARGLVYDAAQKRDAGMRVAQESAMAKYYASEIANEVAYKALQ
                     LHGGYGFIKDYEIERMYRDARIVSIYEGTSEVQKMVISSNVLK"
     gene            complement(9153..10280)
                     /gene="hadC"
                     /locus_tag="PC471_002285"
     CDS             complement(9153..10280)
                     /gene="hadC"
                     /locus_tag="PC471_002285"
                     /EC_number="4.2.1.157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(R)-2-hydroxyisocaproyl-CoA dehydratase subunit
                     beta"
                     /protein_id="HDF2789413.1"
                     /translation="MEAILSKMKEVVENPNAAVKKYKSETGKKAIGCFPVYCPEEIIH
                     AAGMLPVGIWGGQTELDLAKQYFPAFACSIMQSCLEYGLKGAYDELSGVIIPGMCDTL
                     ICLGQNWKSAVPHIKYISLVHPQNRKLEAGVKYLISEYKGVKRELEEICGYEIEEAKI
                     HESIEVYNEHRKTMRDFVEVAYKHSNTIKPSIRSLVIKSGFFMRKEEHTELVKDLIAK
                     LNAMPEEVCSGKKVLLTGILADSKDILDILEDNNISVVADDLAQETRQFRTDVPAGDD
                     ALERLARQWSNIEGCSLAYDPKKKRGSLIVDEVKKKDIDGVIFCMMKFCDPEEYDYPL
                     VRKDIEDSGIPTLYVEIDQQTQNNEQARTRIQTFAEMMSLA"
     gene            complement(10280..11506)
                     /gene="hadB"
                     /locus_tag="PC471_002286"
     CDS             complement(10280..11506)
                     /gene="hadB"
                     /locus_tag="PC471_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(R)-2-hydroxyisocaproyl-CoA dehydratase subunit
                     HadB"
                     /protein_id="HDF2789414.1"
                     /translation="MSEKKEARVVINDLLAEQYANAFKAKEEGRPVGWSTSVFPQELA
                     EVFDLNVLYPENQAAGVAAKKGSLELCEIAESKGYSIDLCAYARTNFGLLENGGCEAL
                     DMPAPDFLLCCNNICNQVIKWYENISRELDIPLIMIDTTFNNEDEVTQSRIDYIKAQF
                     EEAIKQLEIISGKKFDPKKFEEVMKISAENGRLWKYSMSLPADSSPSPMNGFDLFTYM
                     AVIVCARGKKETTEAFKLLIEELEDNMKTGKSSFRGEEKYRIMMEGIPCWPYIGYKMK
                     TLAKFGVNMTGSVYPHAWALQYEVNDLDGMAVAYSTMFNNVNLDRMTKYRVDSLVEGK
                     CDGAFYHMNRSCKLMSLIQYEMQRRAAEETGLPYAGFDGDQADPRAFTNAQFETRIQG
                     LVEVMEERKKLNRGEI"
     gene            complement(11521..12321)
                     /gene="hadI"
                     /locus_tag="PC471_002287"
     CDS             complement(11521..12321)
                     /gene="hadI"
                     /locus_tag="PC471_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyisocaproyl-CoA dehydratase activator
                     HadI"
                     /protein_id="HDF2789415.1"
                     /translation="MYTMGLDIGSTASKGVILKNGEDIVASETISSGTGTTGPSRVLE
                     KLYGKTGLAREDIKKVVVTGYGRMNYSDADKQISELSCHARGVNFIIPETRTIIDIGG
                     QDAKVLKLDNNGRLLNFLMNDKCAAGTGRFLDVMAKIIEVDVSELGSISMNSQNEVSI
                     SSTCTVFAESEVISHLSENAKIEDIVAGIHTSVAKRVSSLVKRIGVQRNVVMVGGVAR
                     NSGIVRAMAREINTEIIVPDIPQLTGALGAALYAFDEAKESQKEVKNI"
     gene            complement(12360..13559)
                     /gene="hadA"
                     /locus_tag="PC471_002288"
     CDS             complement(12360..13559)
                     /gene="hadA"
                     /locus_tag="PC471_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocaprenoyl-CoA:2-hydroxyisocaproate
                     CoA-transferase HadA"
                     /protein_id="HDF2789416.1"
                     /translation="MLLEGVKVVELSSFIAAPCCAKMLGDWGAEVIKIEPIEGDGIRV
                     MGGTFKSPASDDENPMFELENGNKKGVSINVKSKEGVEILHKLLSEADIFVTNVRVQA
                     LEKMGIAYDQIKDKYPGLIFSQILGYGEKGPLKDKPGFDYTAYFARGGVSQSVMEKGT
                     SPANTAAGFGDHYAGLALAAGSLAALHKKAQTGKGERVTVSLFHTAIYGMGTMITTAQ
                     YGNEMPLSRENPNSPLMTTYKCKDGRWIQLALIQYNKWLGKFCKVINREYILEDDRYN
                     NIDSMVNHVEDLVKIVGEAMLEKTLDEWSALLEEADLPFEKIQSCEDLLDDEQAWAND
                     FLFKKTYDSGNTGVLVNTPVMFRNEGIKEYTPAPKVGQHTVEVLKSLGYDEEKINNFK
                     DSKVVRY"
     gene            14236..15234
                     /locus_tag="PC471_002289"
     CDS             14236..15234
                     /locus_tag="PC471_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HDF2789417.1"
                     /translation="MKILVFGARDYEEPVIKKWSEEHKDVQVDIYPENMTEENVVKAK
                     GYDGISIQQTNYIDNPYIYETLKDAGVKVIASRTAGVDMIHFDLVNENGLIVTNVPSY
                     SPNAIAELAVTQAMNLLRKTPLVKKKVCEGDYRWIAELLGTEVRSITVGVIGTGKIGA
                     TSAKLFKGLGANVIAFDQYPNSDLNDILTYKDSLEDLLKEADLITLHTPLLEGTKHMI
                     NKDTLAIMKDGAYIVNTGRGGLINTGDLIEALESGKIRAAALDTFETEGLFLNKKMNP
                     GELTDPEINKLLSMEQVIFTHHLGFFTSTAIENIVYSSLSSAVEVIKTGTATNRVN"
     gene            15596..16123
                     /locus_tag="PC471_002290"
     CDS             15596..16123
                     /locus_tag="PC471_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04002 family protein"
                     /protein_id="HDF2789418.1"
                     /translation="MEKMNKVTSKKTTYIVTSALFASIICLTIAYILHIPVGGNNGYV
                     HIGDAFIYLAATILPTNYAIAASAIGAGLADLSTGAAIWVIPTIIIKPILVLFFTSKS
                     DKIINKRNIVASVVAGIVGLVLYMFAEGIIIGSFTSAFVMSLLGLLQPIGSFIVFIIL
                     GMALDKLDFKKRYFN"
     gene            16150..16746
                     /locus_tag="PC471_002291"
     CDS             16150..16746
                     /locus_tag="PC471_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04100 family radical SAM protein"
                     /protein_id="HDF2789419.1"
                     /translation="MNILYTIENSIYVNITNTCPCSCVFCIRNEKDEVANSGSLWLEH
                     EPSVDEVKEAFNKYNLDDYDEIVFCGYGEPLMRINELIEIAKFIKEKSSIKIRINTNG
                     LSDLIHNKKTAILLKNVIDAVSISLNAPNKEAYNRVTQPKFGEKSFDYMLDFARDCKK
                     YIKEVAFSVVDEISPEEIEESKQLAKKLDIPLRVRHKN"
     gene            complement(16811..18394)
                     /gene="asnB"
                     /locus_tag="PC471_002292"
     CDS             complement(16811..18394)
                     /gene="asnB"
                     /locus_tag="PC471_002292"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HDF2789420.1"
                     /translation="MINLEEKYLTRKDTYMIKILEYNGHIRNWKVLCEELGIDSSLCR
                     DERERAILVEAYKTWGYDMANHMHGMFAFALWDTVEKKLFCLRDQFGVKPFYYYETEN
                     GKLLYGSSIRKIMEQPGFVKELNEDMLQIYMSFTYVAGENTFFRGVKKLMPGHYLVYQ
                     NKTVCIGRYWKPEFHPDHSKSIEEWTDEIHTTLQKIMPEVKSENETAELFLSGGVDSS
                     YILAMSDIEKTGSCGYEEERFDESKLAQQTANILGCKNSRYIITPEEYFASVPYVMYH
                     MEQPTGDASAIVFAIGCKAAAENTNICYSGEGADEFFGGYNIYHKAECYGENLKTFYI
                     GNTNIMNEGEKRKILKKYNPDVLPIEVVKGIYEETEELDTLTNMSNVDIQIWLEGDIY
                     FNIDKMSTAVGLEVRMPLTDTRIFDIASRIPSEYKVKGKENKIAFRTAASKTLPEEIA
                     FRNKLGFIVPIRIWMADDRYNKDIRDKFNSEIADKFFNIDEINEIFNDYISGNSDNWR
                     KIWMIYTFLVWYEIYFVKC"
BASE COUNT         5800 a         3429 c         2290 g         7143 t
ORIGIN      
        1 attaaaactt tttttatttc gtattcccac actccatata tataactaca gacacatcaa
       61 aacacctttt caattcttta ttcctaattt tattaccctc taatctaaat attatatgtc
      121 tatgaaactg tctaaagctc aagatttcta gcattatttt cgacgtagca acactacagt
      181 ctcacaatgt ggcgtatttg ggaacatatc catacactta accatcttaa tctcataacc
      241 atacccctta aaatcaacca aatcaacaac caaagactta ggattacaag aaatataaac
      301 aatctcctta gcaccaaatc cacaaatatc cctaatagca tccttatgaa tgcctggtct
      361 aggagggtca acaataatca aatcaggctt gtcctttaaa tccttaacaa ccttagccac
      421 atccccagca ataaactcac aattagtaag tccattcaac ttagcattct cattagcagc
      481 aacaacagct tcctcaacta tctcaatacc atacactttc ttagcagcac cagccataac
      541 ctgtccaata gacccagtac cactatacaa gtcaaataca actttatcat tgtaatctcc
      601 aataaaatcc ctagcaatac tataaagaac ctctgctccc ttagtattgg tttggaaaaa
      661 cgaaaatggt gaaatcttaa acttaagacc taaaatttct tcttgtatat aatctcttcc
      721 atacaatact cttaactcat cacattggac tgcatctgct aaaccatcat taatagtatg
      781 aagtacacct actaactcag cttttaaatc aatccctaaa agcatatctt taaactcata
      841 catatcaaaa tcttcttgag aagaagttac tatattaacc ataatctcat tagtatgaat
      901 cccttttcta actacaagat gtctcaaata tcctttgtga ttcattgttc tataataagg
      961 aagctttttt tcattgaaaa actctacact agaaactaat atctttgaga agtcttcatc
     1021 aaccaacata cactcctcaa cagtttgtat atctatatgc tttccttttt tatgaagacc
     1081 taatgtcaaa ggtccatttt ttacttcatc tccaaaagta tattccatct tatttctata
     1141 atatttgttt tctggacttc cttctatccc taaaaactga aatccaaata aatcttgctt
     1201 taaaaataaa tccataactt gtttttcttt tatttctaat tgtttttcat atggcacaga
     1261 caatagtgta catcctccac actctctaaa gtgcttgcaa gtttcttcag tttcaagtgg
     1321 tgagttctct aataattcta tcatcttaac ttcagctttc ttacttctaa cttttttaac
     1381 tccagctctc accttttgac cacttattcc acccttcata tggataactc tatctccaac
     1441 ttggcttaaa gatgtaccac caaattccat tttatctact tcaaattcta taacatcttt
     1501 tttcttcaca gacattcctc caatttacta tagtatcttt attatctttc ttcaatcaat
     1561 tttacttctt catctgttaa ctctctatac tcacctaact ctaaactttc atccaatttt
     1621 aaattgccca tagacaatct ttttaagtat actacttttt tgcctacgct ttcaaacatt
     1681 ctttttactt ggtgaaactt accttcatgt attgtaagct ctatttctga ttcatcatca
     1741 ctttttaata tatttaactg tgatggcata gttttatatc cgtcatcaag cactacacct
     1801 tctaaaaaag cttctacatc ttcttcagta actactccat caatttttgc ataataagtc
     1861 ttaggcacat gcttcttagg agataaaact ctatgagaaa gctttccatc attagttaaa
     1921 actaataatc cctctgtgtc tttatctaac cttccaacag gaaatggttc aaaagctaag
     1981 tatgataaat caattaaatc taatactgtt gggtcatatt tatctgtagt tgctgatata
     2041 tatccatcag gtttattcat catcaaatat atatattctc tatatataac ttcttctcca
     2101 ttaaataata tctcatcatt ttcagtgtct acttgagttc ctggatttga aacttcactt
     2161 ccatttacta ctacagaccc ttgtttacaa tacttcttta tttcacttct acttccataa
     2221 cctaaatttg aaagaatttt atctatacgt tgctttttag ccattaaaaa tgctctccat
     2281 tctcataata tttttctttt tcaaatactg taaatgctaa atctatactt tctacatcta
     2341 aattatttct tatactatca gttattattc ttgctactat atcttgtact tcttgtcctc
     2401 tatcaaacca agctacttca acaaaaggat atacatctgc tattttacca tctcttattg
     2461 caactgactt tatacattct atttcaaagt agtctcttgg acattttaca gcttcaacta
     2521 aatcatttat cattttttca cttattttac atatatcatt ttcattaatt cctcttattt
     2581 ttatctgtgg catagtttat ctcctttatt ttaaattttt agtgaattat catccactta
     2641 attcctttcg ataacgtttg aagtggtaac ttcttggtca aggcaactat tgttatcagt
     2701 atctccaaac taataaggta gttcctaccc ttatagttag cattatatag ctagttttcc
     2761 taaattgttt atattcttta ttattttatt taatcctatt gtcttctatt taacattata
     2821 cttgtaattt atagttatta caatttacta tatacttttt ttgatttaac gttataatat
     2881 aagtgatggg taaattttaa aagttaatag gaggagttta aaatggcatt aattactaca
     2941 aaagaaatgt ttaaaaaagc ttacgaaggt ggatttgcta taggtgcatt taacataagt
     3001 gacttagaac aattacaagg tgttttaaaa gctgctaaag ctaaaaattc ttatgttatg
     3061 atacaagctt ctatgtcagc tgttaaatat gctggacctc atacattagt tgaaatggtt
     3121 aaggctgctt ctgatgaaat aggtgtagat gtagctcttc acttagacca tggtccaaat
     3181 atggatgcta ttaaaacttg tattgatgct ggtttctctt cagttatgat agatggttct
     3241 cattttgact ttgaagaaaa tgttagaata actaaagaag ctgttgaata tgcacactct
     3301 aaaggtgttg ttgttgaagc agaacttggt gttctagctg gaacagaaga tgatgttact
     3361 tctgatgttc acaagtatac tcaaccagct gaggcagttg agttcgttga aagaacaggt
     3421 gttgattcat tagcaatagc tattggaact tctcatggtg ctttcaaatt caaaggagaa
     3481 gctaaattaa gattcgatat actagaagaa atccaaagca aattaccagg tttcccaata
     3541 gttcttcatg gtgcatctgc tgttgaccaa aacgctgttg ctacatgtaa tgaatttggt
     3601 ggaaatatag ctggtgctaa aggtgtacct gtagatatgt taagaaaagc atcttctatg
     3661 gctgtttgta aaataaacat ggatactgac ttaagactag ctatgactgc tgctatcaga
     3721 aaattcttag ctgaaaatcc aaaagaattt gacccaagaa aatacattgg tgctggtaga
     3781 gatgctatac aagctgtagt tgaaagcaag atagatgatg ttttaggttc tgctaactct
     3841 ataaactaat ataactttta aatatttata aaagaaaaga gatgacttaa agtaattaaa
     3901 tttattttta agccatctct tttaagttgt ataaattatg tatcttataa tattttaatt
     3961 atattgtctt tttaaactga cagctaaacg tgaatatgtc cactctttat ctttctatgg
     4021 atggtagaag gatatatacc tataacttca gcagcctttg ttattgaacc atacttattt
     4081 atagccttac ttatcattat ttggtccact attttatatg catcattaag attcataatt
     4141 ccatttacca ctattggtga taaatcatct gtttcattat caatcatatc taaatttatc
     4201 agcatgttaa attcatcttc tccaattaca tttttagctg ataatactac aaatctttca
     4261 atgatgtttt ttaattctct tatgtttcct ggccaagaat attcttctaa taactcaata
     4321 actttaggcg aaacttctac ttctctattg tacttttcat tgtaaagttt taagaaataa
     4381 tttactaatg gtactatatc ctcttttctt tctctaagag gaggtatttt tactggaata
     4441 acacttaatc tatagtacaa atcttgtcta aatttaaggt tatttctaag gtcttcatga
     4501 gaaatattag tagcacttat atatctaaca tctattttta taggcttact accacctatt
     4561 ctagtaatag tattttcttg tataacccta agtaattttt tttgcatctg cattggtaac
     4621 tcacctatct catccaagaa aatagttcca ccattcgcct cttcaaaaag tcccttttta
     4681 ccttttttct tagctccagt aaaagaacct tcttcatatc caaaaaactc tgactctaat
     4741 aattcatttg gtatagctcc acagtttata gctataaaag gtttgtctct ccttgaacta
     4801 tacttatata ttgttcttgc tattatttct tttcctacac ctgattctcc atttataaat
     4861 atagaagaat ctgaggctgc agctctctta acaacttttt ttatattgtc cataatctta
     4921 ctttgactta tatactctgt gtcttctgat atatcactat attttatctg agattcaagc
     4981 ttaataaccc ttgaaatatc tctccctgtt agtactcctc ctgtaagctt tcccttagaa
     5041 tcccatcttg gaactgccgt atatgcaata agcttcccat ctgggtaaac gatatttcta
     5101 taaatctttt tcttcacttt aagcatgttt aatattattg gttcttcaac taatccttgt
     5161 ctcacaaggt ctgttatatt tctaccaagt acctcagttc ttttaaaagg taatatctca
     5221 tcactgacag tgttcatctt ttcaattacc ccatctttgt taaacacaac aacaacttca
     5281 tctatatgct ctattatatc ttcaattact ttatcagata ttagagactt ctcaaataaa
     5341 taaactatat tattttcatt attattatct ttcatattgt atatatgtgc agtcatcctt
     5401 aactctctga aagctatttt ctttttacta tctgtcagta tttctgtttt gtcaaaattt
     5461 gaatctaatt catttatatt tctaggcttt tttatatcta gaaaactaaa aatactatct
     5521 attagatgat tacagtatat tatttcgcct gattctttac ataaaatggc tggattttct
     5581 acatgttcta taagccctat taaattatta tccattaata ttcgcacctt tcattaagta
     5641 atttattgaa ctacatacaa aatttattgt ttgcttacaa taaataccat cagcacatat
     5701 aatcaactcc ttataatatt cgtcttttca agtagtttat ttcattaaaa cgtaactaag
     5761 atataatttt aatgattata gtttgttaaa aatttcattt tgcttttaat tgtattttat
     5821 cacatattta gtaattattt gtctatttaa tacaatttaa ttaaatttaa gatttattta
     5881 aaaaaacaaa gataatcctt acagaatata actcacaatt tgtaagcaat atacatataa
     5941 ggattatcca gattatcttg tattataaaa ttttattcaa acagactttt taataactga
     6001 caaatacaca ctagcaataa cttatcaaag ggttaataat tatgttacta actaataaga
     6061 cttattcaat cagtaatatt tatatagttt gtatctatta agcttttata gcttctaata
     6121 aatcatcaac gctatctaat ttttctataa gcattggaac tatttcatgt aagtctccaa
     6181 ctactccata gtcagcgatt tctaaaattg gagctgctgg gtttttgttt atagcaatta
     6241 tgaaatctga tgattgcata cctgctaaat gttgtattgc tcctgatata ccacaagcta
     6301 tatataggtt tggtttaaca gtagttcctg tttgacctac ttggtgagaa tggtctatcc
     6361 atccagcatc aacagccgca cgagaagaac caactactcc acctaatttg tcagctaatt
     6421 tcttaagcat ttcaaatcct tctggtccac ctaatcctag accacctgat actataacat
     6481 ttgcatctgt aagagatact aaatctttct tagttttaac tgtttctaaa acagtagttc
     6541 ttatatcatc ttgagttatt ttgtagtcta atgctataac ttcaccagtt cttgtttcgt
     6601 ctttttcagc tttatccata acaccaggtc taactgtaga catttgaggt ctatggtttg
     6661 gacaaatgat tgtagccatt atgtttccac caaatgctgg acgagtttgt tttattttct
     6721 tatcttctgg gtctatttct aacttagtac agtcagctgt taatccagtt ccaactcttg
     6781 aagctattct aggtgctaag tctctaccta tatgagttgc tccgaaaagc attatttctg
     6841 gttttatttc atctattgca tcttttatta cttttgtata tgcatcagtt gtatattttt
     6901 ctaaaagagc atcatctgca acataaactt tgtcagctcc aaaagtgatt aattctttag
     6961 ctaatccatc aacatctttt cctaataata ttgcacaaag ttctgcatct acttctttag
     7021 ctaattttct tccttctcct aataattcta taactactgg agctattttt ccttctcttt
     7081 gttctgcaaa tatccatacg tttttataag aacttaaatc ttttatatca ttcatagttt
     7141 gtactcctcc aacttatata atgtgctttt gttttagacc gattattatt ttactagcta
     7201 tttcatcagc tttaccttct aagatttctc cttttcctct tggttcagga gtaaatgatc
     7261 taaatacttt agttggtgat gcttttaaac caacttcttc tttatttaca tctaaatcat
     7321 caattgtcca aacttttaca tcatgtttat atgcttttac tattttatca acagacatgt
     7381 atctaggtgt atttaactct tttactgctg ttaaaagtac aggtgtactt acttttatta
     7441 attcgtatcc atcttctaat tgtctttgaa ctattatatc ttttccttct attttgaaat
     7501 cttgtacata tgtaacttga ggaatgtcta atttctctgc aatttgtggt ccaacttgtg
     7561 cagtatctcc atctattgct tgtcttcctg caaatattat gtcatagtca cctactttag
     7621 atattcccgc tgcaatagta tttgatgtag cccaagtatc agctcctcca aatgctctat
     7681 cacttaataa tattgcatca tcagcaccca tagctaaaca ctctcttagc ataaagtctg
     7741 cttgaggagg tcccattgtt ataactgtta tagttacatc atcatgctca tctttaattt
     7801 ttaaagcttc ctctaatgca tttgcatcat ctgggtttaa tatacttgga actccatctc
     7861 ttataagagt acctgtttct ttatttattc ttacttcgtt agtatctgga acttgtttta
     7921 cgcaaactaa tattttcaaa gtctatacct ccaatttcat tagattattt attattttaa
     7981 tacgtttgat gaaattacca ttttttgaac ttctgatgtt ccttcgtata ttgatacgat
     8041 tctagcatct ctgtacattc tttcgatttc ataatctttt ataaatccat atccaccgtg
     8101 aagttgtaat gctttataag caacttcatt tgcaatttct gatgcatagt atttagccat
     8161 agcagattct tgagcaactc tcattcctgc atctctcttt tgtgctgcat cataaactaa
     8221 tcctctagct gcacaaactt ttgtttccat gtcagctata gtaaattgag tattttggaa
     8281 tttagctata ggcttaccaa attgaactct ttgttttgta tatttaacag cttcatctaa
     8341 agcaccttga gctattccta gagctaaggc agcaacacct attctaccaa cttctagagt
     8401 acccattgct atcttaaatc cttgaccttc ttttccaagt aagttttctt ttggaacttt
     8461 aacgttttca aatattaagt cagaagtttc agttcctctt atacccatct tatcttcatg
     8521 agctcctgtt gagaaaccat cccatttact ttctactata aacgctgatg ttcctctaag
     8581 tcctttactt ctatctgtaa ctgctattac tatagctaca tcacagaatg gtccattagt
     8641 tatgaaagtt tttctaccat ttaatatata gtggtctcca actaattctg ctgttgtttg
     8701 ttgtccacct gcatcagaac cagctcctgg ctcagttaaa ccaaatgctc ctattaattc
     8761 acctgttgct atacctttta agtatttttg tttttgctct tcagtaccat tgtaaagtat
     8821 tggtaatgaa gcaagagaag aaccagctgt aacaaaagta gctgttgaag cacatcttct
     8881 agctatttct tccattacta tagcgtaaga aacatatcct gctcccccac caccatattc
     8941 ttctgggata ttactgctta taaattttgc atcagctaat tttttgatta attccttagg
     9001 cattacacct gttttatcca tttctgctgg taaagtatct aattctttac taacaaaatc
     9061 tcttactgct tttcttaaaa gttcttgttc tttgttatat aacatagctt cctccaattt
     9121 tcatatttta tagtaaattt attttaatga acttacgcta aactcatcat ctcagcaaaa
     9181 gtttgaatac gagttctggc ttgttcatta ttctgagttt gttggtcgat ttcaacatat
     9241 aaagtaggta ttccactatc ttctatatct tttctaacta aaggataatc gtattcttct
     9301 gggtcacaga atttcatcat acagaagata acaccatcta tatctttctt tttaacttca
     9361 tctactataa gtgacccacg ttttttctta gggtcataag ctaatgaaca tccttctatg
     9421 tttgaccatt gtcttgctaa tctttctaac gcatcatcac ctgctggtac atctgttctg
     9481 aattgtcttg tttcttgtgc taagtcgtca gctacaactg atatattgtt gtcttctaaa
     9541 atgtctaata tatctttaga atcagctaat atacctgtta ataaaacttt ctttccagaa
     9601 cagacttctt ctggcatagc atttaatttt gctattaaat ctttcactag ctcagtatgt
     9661 tcttcttttc tcataaagaa cccactctta attactaagc ttcttattga tggttttata
     9721 gtattagaat gtttataagc tacttcaaca aagtctctca tagtttttct atgttcattg
     9781 taaacttcta tactttcatg aatttttgct tcttctattt catatccaca aatttcttca
     9841 agttctcttt ttacgccttt gtactcactg attaagtatt ttacaccagc ttcaagtttt
     9901 ctattttgtg ggtgtactaa tgatatatat tttatatgag gtactgctga tttccagttt
     9961 tgtcctaaac aaattagtgt atcacacata cctggtataa taactccaga taattcatca
    10021 taagcacctt ttaatccata ttctaaacat gattgcatta ttgaacatgc aaatgcaggg
    10081 aaatattgtt tagctaaatc taattctgtt tgccctcccc atataccaac tggaagcatt
    10141 ccagctgcat gtataatttc ttctgggcaa taaactggga aacaacctat agctttttta
    10201 ccagtttcac ttttatattt ttttacagcc gcatttggat tttcaactac ttctttcatt
    10261 ttagataaaa tagcttccat tatatctcac ctctattaag tttttttctt tcttccatta
    10321 cttcaactaa accttgaatt cttgtttcaa attgagcatt agtgaaagct ctagggtctg
    10381 cttggtcacc atcaaatcca gcatatggta atccagtttc ttcagctgct cttctttgca
    10441 tttcatattg tattaaactc ataagtttac agcttctgtt catatgatag aatgctccat
    10501 cacatttacc ctctactaaa gaatcaactc tatattttgt catacggtct aggtttacat
    10561 tgttaaacat agtactatat gctactgcca ttccatctaa atcattaact tcatattgta
    10621 atgcccaagc atgtgggtaa acactacctg tcatgttaac tccaaattta gctaatgttt
    10681 tcatcttgta tcctatatat ggccaacaag gtataccttc catcattatt ctgtattttt
    10741 cttcccctct gaaagaagat ttaccagttt tcatgttgtc ctctaattct tctataagta
    10801 acttaaatgc ttctgtagtt tcttttttac ctctagcaca aactattaca gccatgtaag
    10861 taaataagtc aaatccattc attggagaag gagaagaatc tgctggtaaa ctcatagaat
    10921 acttccatag tcttccgttt tcagctgata ttttcattac ttcttcaaac ttcttagggt
    10981 caaatttctt tcctgatata atttctagtt gttttatagc ttcttcaaat tgagctttaa
    11041 tataatctat tcttgattga gtaacttcgt cttcattatt gaaagttgta tcaatcatta
    11101 ttaaaggtat atctaattct cttgaaatat tttcatacca ttttataact tggttacata
    11161 tattgttaca gcaaagtagg aaatctggag ctggcatatc caaagcttca catccaccat
    11221 tttctaaaag accaaaattt gttcttgcat atgcacatag gtcaatagaa tatcctttag
    11281 attcagctat ttcacataat tctaaagaac cttttttagc tgctactcca gctgcttggt
    11341 tttctggata taatacgttt aagtcaaata cttctgctaa ctcttgagga aatactgatg
    11401 ttgaccaacc tacaggtctt ccttcttctt tagctttaaa tgcatttgca tattgttcag
    11461 ctaataaatc attaattact actctagctt cttttttttc agacattttc atccctcttt
    11521 ttatatattt ttcacttctt tttgtgattc ttttgcttca tcaaaagcat ataacgctgc
    11581 tcctaaagca ccagttaatt gaggtatatc aggtacaata atttctgtgt tgatttctct
    11641 tgccatagct cttacaatac cactatttct agcaactcca ccaaccataa ctacatttct
    11701 ttgtactcct attcttttta ctaggctaga aactctcttt gctactgaag tatgaatacc
    11761 tgccactata tcttcaattt ttgcattttc agataaatgt gatataacct cagactctgc
    11821 aaatactgta catgtactgc ttattgatac ttcattttga gaattcatag atatacttcc
    11881 gagttcagat acatcaacct ctattatttt tgccattaca tctaaaaatc ttcctgtacc
    11941 tgcagcacat ttgtcattca taagaaagtt taatagtctt ccattattat ctaattttaa
    12001 tacctttgca tcttgaccac ctatgtcaat aatggttctt gtctctggaa ttatgaaatt
    12061 tacccctcta gcatgacagc ttaattcact tatttgctta tcagcatctg aatagttcat
    12121 tcttccatat cctgtaacta caactttttt aatatcttct cttgcaagac ctgtcttgcc
    12181 atataatttt tctaaaactc ttgatggtcc agtagtccca gtaccagagg atattgtttc
    12241 agaagctaca atatcttccc cattctttaa gattactccc tttgatgcag ttgaacctat
    12301 atctaatccc attgtgtaca tctttagcat tctcctttta aaaaatctag aattactaat
    12361 taatatctta caactttact atctttaaag ttatttattt tctcttcatc gtagcctaaa
    12421 gattttaata cttctacagt atgttgacct acttttggtg ctggtgtata ttctttaatt
    12481 ccttcatttc taaacataac tggagtatta actaagacac ctgtatttcc gctatcgtat
    12541 gttttcttaa ataagaagtc atttgcccaa gcttgttcgt catctaataa atcttcacag
    12601 ctttgaattt tttcaaatgg taagtctgct tcttctaata aagctgacca ctcgtctaat
    12661 gttttttcta acatagcttc tccaactatc ttaactaaat cttcaacatg attaaccatt
    12721 gaatctatgt tattatatct atcatcttct aatatatatt ctctatttat aaccttacag
    12781 aatttgccta accacttgtt gtattgtatt aaagctaatt gaatccatct tccatcttta
    12841 catttatatg tagtcattaa tgggctgttt ggattttctc ttgataaagg catttcattt
    12901 ccgtattgtg ctgttgttat cattgttccc attccatata tagctgtatg gaaaagactt
    12961 actgttactc tctcaccttt accagtttga gcttttttat gtaatgctgc taaacttcct
    13021 gctgctagtg ctagacctgc atagtggtca ccaaatcctg ctgctgtatt tgctggagat
    13081 gttccttttt ccataacaga ttggctaact cctcctcttg cgaagtatgc agtatagtca
    13141 aatcctggtt tatcttttaa aggtcctttt tcaccatatc ctaatatttg agagaatatt
    13201 aatcctggat acttatcttt tatttggtca taagctatac ccattttttc taatgcttga
    13261 actctaacat tagttacaaa tatgtctgct tctgataata atttatgtaa tatttctact
    13321 ccttcttttg attttacatt aatacttaca ccctttttat ttccattttc taattcaaac
    13381 atagggtttt catcatctga tgctggagat ttaaatgttc cacccataac tcttattcca
    13441 tcaccttcta taggttcaat cttaataacc tctgcacccc agtcacctaa catttttgca
    13501 caacatggtg ctgcgatgaa acttgaaagt tctactactt taactccttc taaaagcata
    13561 acgttttcct ccttaatagt atcaattata aaatttcgat taaatcactc ataaaatact
    13621 tttttctgat attaactgtc tgatgtcaaa gttcctaatc ttatctaaaa taacttaaaa
    13681 attattaaaa ttatttttta ttgttttatt gtcttacacc tatattgtta agcatttatc
    13741 gtgccaactt tatatttaat caaaatatcg cttgatttga atgtttttta acaatatgca
    13801 attgaataat ctttttcata tcgtggcatc ttgccactat ccattgcatt ttgctattct
    13861 ttctatgctt ataaaagttt tttttatata tttttttatg aaaaagcttg acatttcaaa
    13921 aaaagttttc attgctaaac tcaaaaacac atgttatcat tgtaattaat cagcaagtta
    13981 gttaactaat ttcaaactta gataattttt taataatatt taatctaaaa aataacaatt
    14041 tctttgataa attttagagt aattattaca ttaaataaaa aattactaaa tatcaaaata
    14101 acacagggaa aacaatttca aaactaaatt tttaacctta aaaattttgt ataaaaaata
    14161 taaccttata tgctacatat tatttctaaa tgaaaaatta ccataataat ttaataatta
    14221 caggagggta ttgatatgaa aatactagta tttggagcac gcgattatga agaaccagta
    14281 ataaaaaaat ggtctgaaga acataaggat gttcaagtgg atatttatcc tgaaaacatg
    14341 actgaagaaa atgtagttaa agctaaaggg tatgatggta tatctataca acaaactaac
    14401 tatatagata atccttatat ttatgaaact ttaaaagatg ctggggttaa agttatagct
    14461 tcaagaactg caggggttga catgatacat tttgatttag ttaatgaaaa tggacttatc
    14521 gttacaaacg ttccttctta ttcacctaat gcaatagctg aattagctgt tactcaagct
    14581 atgaaccttt taagaaagac tcctctagta aagaaaaaag tctgtgaagg tgattaccgt
    14641 tggatagctg aacttcttgg aacagaagtt agatctatta cagttggtgt tataggtaca
    14701 ggaaaaatag gtgctacttc tgcaaaatta ttcaaaggcc taggagctaa tgtaattgca
    14761 tttgaccaat atccaaatag tgatttaaat gatatattaa cttacaaaga ttctttagaa
    14821 gaccttctaa aagaagctga ccttataaca ttacatactc ctttacttga aggaacaaaa
    14881 catatgataa ataaagatac tctagctata atgaaggatg gagcttacat agtaaatact
    14941 ggccgtggtg gtttaattaa tacaggggat ttaatagaag cactagagtc aggaaaaatt
    15001 agagctgctg cccttgatac atttgaaact gaaggattgt tcttaaacaa aaaaatgaat
    15061 cctggagaat taactgaccc agaaataaat aaacttcttt ctatggaaca agttatattc
    15121 actcatcacc ttggtttctt cactagtaca gcgattgaaa atatagttta ttctagttta
    15181 agtagtgctg tagaagttat aaaaacagga actgctacta atagagtaaa ttagtatttt
    15241 ataaatatat tttttcatta attagctatt atctaacaaa atagccttta atttcccaaa
    15301 tataagagag tcagctcagt atatattgag tagactctct tatattattt tttatattaa
    15361 acttgtactc ttcttattca taccaaatta aatttatatt aacttattct aactactcct
    15421 actctataca ttttacctat ttttactttt ctaattatat acatttgaat gggtacaatc
    15481 tgtattttac aaaaaaaaca cctaacaaac tatgaaaatt gtatgattaa tattaatata
    15541 tttataagaa aacataacaa ttatgttagc tctggatttt tagggaggga gtatattgga
    15601 aaaaatgaat aaagttactt ctaaaaaaac tacctatatt gttacttcag cattatttgc
    15661 atccataata tgcctgacaa tagcatatat attacatatt ccagttggtg gaaacaatgg
    15721 atatgttcat ataggagatg cttttattta tctagctgca actattttgc caacaaacta
    15781 tgctatagct gcttctgcaa ttggtgctgg tctagctgat ttatctacag gtgctgctat
    15841 ctgggtaata ccaactataa tcataaaacc aatacttgta ttatttttca cttctaaaag
    15901 tgacaagata ataaataaga gaaatatagt tgcttcggtt gtagcaggta tagtaggatt
    15961 agttttatac atgtttgctg aaggtataat tattggaagt tttacaagtg catttgtaat
    16021 gagcttactt ggcttattac agccaatagg aagttttatt gtttttataa tcttaggtat
    16081 ggcacttgat aaacttgatt ttaaaaaacg ttattttaat taagaattta aatttatgga
    16141 gggaaaatta tgaatatctt atatacaatt gaaaatagta tctatgtaaa tataactaat
    16201 acatgtccat gtagttgtgt tttttgtata agaaacgaaa aagatgaagt tgcaaatagt
    16261 ggtagtttat ggttggagca tgagccaagt gtagatgaag taaaagaagc cttcaataaa
    16321 tacaatttag acgactatga tgaaatcgta ttttgtggat atggcgaacc attaatgaga
    16381 ataaatgaat tgatagagat agcaaagttt ataaaagaaa aaagtagcat aaagataaga
    16441 ataaatacaa atggtctaag tgatttaata cataacaaaa aaacagctat tctgttaaaa
    16501 aatgttatag atgcagtatc aataagtctt aatgccccaa ataaagaagc ttataataga
    16561 gttactcaac caaagtttgg agaaaaatca tttgattata tgttagattt tgcaagagat
    16621 tgcaaaaaat atataaaaga agtagctttt tcagtagtag atgaaatatc tccagaagaa
    16681 atagaagaat ctaaacaact ggcaaaaaaa cttgatatac cattaagagt tagacataag
    16741 aattagcaag taaaattata aaacattaag aatcacctga atggtatttc aggtgattct
    16801 tcctcttgta ttaacatttt acaaaataga tttcatacca cactaggaat gtgtaaatca
    16861 tccaaatctt tctccaatta tcagagtttc cactaatgta gtcattaaaa atctcattaa
    16921 tctcatctat gttaaagaat ttatctgcta tctcactatt gaatttatct cggatatcct
    16981 tattatatcg gtcatcagcc atccatatac gaattggcac aataaatcct aatttatttc
    17041 taaaagcaat ctcttctggt aatgtttttg atgcagctgt acgaaaggca attttattct
    17101 cttttccttt cactttatat tctgatggta ttctggatgc aatatcaaat attcttgtat
    17161 ctgtaagagg cattctgact tccaatccta ctgcagtact cattttatct atattaaaat
    17221 aaatatctcc ttccaaccat atttgaatat ctacatttga catgttagta agtgtatcta
    17281 gttcttctgt ttcttcataa attcctttta ccacttcaat aggaagcaca tcaggattgt
    17341 atttcttaag aatttttctt ttctcaccct cattcataat gtttgtattt ccaatataaa
    17401 atgtcttcaa attctctcca taacactctg ccttatggta tatattatat ccaccaaaaa
    17461 actcatctgc tccttctcca gagtaacaga tatttgtgtt ttcagctgct gccttgcatc
    17521 caatagcaaa aacgatagca gatgcatctc cagttggttg ctccatatgg tacattacat
    17581 aaggtactga cgcaaaatac tcttcaggtg taattatata tctcgaattt ttacacccaa
    17641 gaatattagc tgtctgttgt gctaattttg attcatcaaa acgctcttcc tcataaccac
    17701 atgaacctgt tttttctata tctgacattg ccaatatata agatgaatca acacctcctg
    17761 ataggaataa ctctgcagtt tcattctctg attttacctc tggcataatt ttctgaagtg
    17821 ttgtatgaat ttcatccgtc cattcctcaa tagacttgct gtggtcagga tgaaattcag
    17881 gtttccaata acgaccaata catactgttt tattctgata gacaaggtaa tgtcctggca
    17941 taagcttctt tactccacgg aagaatgtat tttcacctgc tacatatgtg aaagacatat
    18001 aaatctgtaa catgtcttca tttaactctt tcacaaatcc tggttgctcc ataatcttac
    18061 gaatagatga accatagaga agcttcccat tttcagtctc ataataatag aaaggtttta
    18121 ccccaaactg gtctcttagg cagaagagtt tcttttccac agtatcccat aatgcaaaag
    18181 caaacattcc atgcatgtga tttgccatat catatcccca tgtcttatat gcttccacta
    18241 aaattgctcg ttcacgctca tctctacata atgaagaatc gattccaagt tcttcacaaa
    18301 gaactttcca gtttcgaata tgcccattgt attctagaat ttttatcata tatgtgtctt
    18361 ttcgtgttaa atacttttct tctaaattta tcatatgtgt atcctttcgt attaaaacat
    18421 ttaacttcta aatttattaa atgtttatat atatttattc acttataatc actgaaaaat
    18481 cgactttttt caaagtaaat tctttttgaa ttagtactaa tctgctcttt attaatggca
    18541 taaatatgat tatcaagtat tatcagtgtt ataacattga attttttatt cttcataaat
    18601 attgccccct ttatattacc aactaaatat aatatatctt atgttactta catattcttt
    18661 aa
//
LOCUS       DAMJYR010000066        18516 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.066, whole genome shotgun sequence.
ACCESSION   DAMJYR010000066 DAMJYR010000000
VERSION     DAMJYR010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 18516)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18516)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18516
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.066"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(<1..591)
                     /locus_tag="PC471_002293"
     CDS             complement(<1..591)
                     /locus_tag="PC471_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HDF2789421.1"
                     /translation="MLNETRKKQLTEVCQDLIRNASYSGQEENVVKAIEENFALLGFD
                     SWSRDRYGNIVGCIKGNKPGKKILFDGHIDTVPVPDASKWSVPPFEARIVDGKIYGRG
                     TSDMKGQVSAMMAAVSYFAEDTNKDFEGELYVAGVVHEEIFEGVSAREISKAVQPDYV
                     VIGESSELNLKIGQRGRGEIVVETFGKPAHSANPEKG"
     gene            complement(921..2135)
                     /gene="dpaL"
                     /locus_tag="PC471_002294"
     CDS             complement(921..2135)
                     /gene="dpaL"
                     /locus_tag="PC471_002294"
                     /EC_number="4.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopropionate ammonia-lyase"
                     /protein_id="HDF2789422.1"
                     /translation="MREIKWKLNNLPKSEEKEKGIEFLKDEEIAKAKAFHESFPQYEK
                     TPLVKLDNLAKSLGVSGIYVKDESYRFGLNAFKVLGGSYSMGRYLAQRLDTDISELGY
                     DKLTSKEIKEKLGEITFFTATDGNHGRGVAWTANKLGQKSVVLMPKGSSEFRLNKIKG
                     EGADASITDLNYDDAVRLANDYAEADDHGVMVQDTAWDGYEEIPAWIMQGYGTMAKEA
                     IEQLKEYGVDRPTHVFVQAGVGSLAGAVQGYVASIYDECPITVVVEADEADCYYKSAE
                     AGDGKPRFVGGDMPTIMAGLACGEPNTIGFEVLKNYSSAFVSAPDWVSAKGMRILGNP
                     LRGDEKVISGESGAVTTGALVSILESEDLKDLREALKLDENSKVLLISTEGDTDPDKY
                     RDIVWNGECQSK"
     gene            complement(2758..3297)
                     /locus_tag="PC471_002295"
                     /pseudo
     CDS             complement(2758..3297)
                     /locus_tag="PC471_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427977.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2'-5' RNA ligase family protein"
     gene            complement(3374..5179)
                     /locus_tag="PC471_002296"
     CDS             complement(3374..5179)
                     /locus_tag="PC471_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDF2789423.1"
                     /translation="MLSKLKEFQQEMIKYTETVASVLDVDIEIVDDRLIRISGTGLYK
                     SKINESVVTEGFIYDNVIQTGQELVVLDICDNQLCIECSHYMKCLNKVIIAVPIKYNN
                     RTIGVIGAISTDKTKKVEISAKIDNYLKFVNHICDLISMKIEEHEASKNSSRKMDMMI
                     EIIENVEKGVIILDINSKISYINNIALKKLDIDKNIIENIVNIVSVESSSNGHELLEI
                     DIDNKIYNINAKIIPVYPYINQYDKIIIFDKTYINHKGHGKVNSGWGNSDIESIIGNS
                     EAMLKVKERTKKLAKSNSTVLITGESGTGKELIARAIHAEGSRWNKPFIAINCAAIPE
                     NLLESELFGYIKGAFSGASSGGKVGKFELANEGVIFLDEIGDLSMPLQAKLLRVLQER
                     KFARIGSNKLIDLDIRVIAATNKNLLKLVNEGKFRDDLYYRLNVIPINLPPLRERKDD
                     IEAIMMKFASKYSLELGIQLNKIEENVMNMLINYNWPGNIRELENAVEYMMNLVGDDG
                     IIYKDMLPLDILNYYNINGNIYKNKDVNIIFEDDIVGGIVENQERILSIKELELTYIN
                     KLLNKYGRDTKTKKKIAKDLGIGLATLYRKLEEEQ"
     gene            complement(5575..5952)
                     /locus_tag="PC471_002297"
     CDS             complement(5575..5952)
                     /locus_tag="PC471_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003483933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HDF2789424.1"
                     /translation="MSKVVIATEKAPGAIGPYSQAIQVGNIVSTSGQLPIDMKTGELV
                     SDPKEATKASLDNVKAILEQAGTSMDKIFKTTVFVKDLKDFNDINEVYATYFKENPPA
                     RSCVQVAQLPKDAVLEIEAMAVL"
     gene            6857..7342
                     /locus_tag="PC471_002298"
     CDS             6857..7342
                     /locus_tag="PC471_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2789425.1"
                     /translation="MSKSELTAETTEEMLEVLSGKDYDIACHLHELGKSLDCKIEPKT
                     GARSYKIVYSTKKPKRSLFTIECNEKKWRVKANLFHLNTYKDAVEECSKTIKDSITKT
                     RTCKKCNSKCIGGSSFELDGKSYLTCIGSGHYFANMEEMDWKNLEKLITKENNIMQES
                     V"
     gene            complement(7542..8081)
                     /locus_tag="PC471_002299"
     CDS             complement(7542..8081)
                     /locus_tag="PC471_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HDF2789426.1"
                     /translation="MSKNVLIVSASPRKGGNSDLLCDQFIQGVLESKNQVEKIFLKDK
                     DINYCTGCGACYEKEANCSQTDDMGEILEKMIEADVIVLATPVYFYTMNAQMKTMIDR
                     TCSRYTEICDKDFYFIVTAADENVADMERTIEGFRGFTSCLNGSKEKGIVYGVGAWNV
                     GDIKGSKAMKQAYEMGKLA"
     gene            complement(8074..8487)
                     /locus_tag="PC471_002300"
     CDS             complement(8074..8487)
                     /locus_tag="PC471_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDF2789427.1"
                     /translation="MTISEVSKKYELSADTLRYYERIGLIPPVNRNKSGIRSFTEEDC
                     EWVNFIKCMRGAGLSIETLIEYVAMFQQGSSTIKARKELLIEQRNQLAKRIEEMQKTL
                     ERLNFKIDRYEEGIIEKEKVLKSSRNKKMEAVSYE"
     gene            complement(8523..9644)
                     /locus_tag="PC471_002301"
     CDS             complement(8523..9644)
                     /locus_tag="PC471_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HDF2789428.1"
                     /translation="MEAKKLGFGLMRLPIKDENDVTTIDMEHLNKMVDTFLERGFTYF
                     DTAYVYHMGKSEIALRESLVKRHKRESFTIATKLPLMALKKKEEQETIFNEQLEKCGV
                     DYFDYYLLHNIGVSHYEVAKKFDSFKFIEEKKKEGKIKNIGFSFHDSAELLDKVLSEH
                     PEVDFVQLQINYLDWDNESIQSRKCYEVAEKHNKPVIVMEPVKGGTLAKIPEKAEKLL
                     NDYNPDMSISSWAIRFAASKDNVMMVLSGMSNMEQLLDNTGYMQNFKPFVQEEYNIID
                     EAVEIINESILVPCTACQYCVEGCPKNIAIPNYFALYNAEKQALNTGFSTQMVYYNNY
                     TKTYGKASDCIECKQCEESCPQHIKIIDALKNVAETFEK"
     gene            10232..11884
                     /locus_tag="PC471_002302"
     CDS             10232..11884
                     /locus_tag="PC471_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp21"
                     /protein_id="HDF2789429.1"
                     /translation="MNKKNLAILISGIMFFSSSSAIFADNTTKQERLIGKTRYETAVE
                     VSKLGWVQSKTAIIVNGNSIQSALCANPFAKLKNAPILLVNNNSIENSTKAELKRLGV
                     DNVYIVDSGNSISSKVENEIKSLNIKINKIVGNNIYEMSTNVLKEIDKIKKVENVAVV
                     NGLKGLSDAVSIASPAAIHNMPIILVSDENSNDSSINFLNNRSIKKSYIVGGNKILSD
                     NTVKKYPNRERISGSDRNNTNAKIIDKFYKDKKINNVFVAKDGINQENALVDSLSIGV
                     LAAKENSPIMISNGSLVNSQKEFIKNKDIDEITQVGGGYNQKAFEDILKLKNVNIRNK
                     TKKISLIQAKEILKQALPGSNILVCYYDEDDNEYDCKVIKDNILYEIEIDAYSGKIIE
                     IEKDDDLNDNNLGTQNISLDKAKSIALDAVPNGNLIYCKYDAEDNEYEAKVIKNKKTY
                     ELEISAFDGRIVDIDMDNDSDDDFDDNDKNNSNTNDNSSIISAEKAKSIMLDKVPGGK
                     IVKFKYDEDDKEYEGEIIKGKTEYEITINAITGKILEFEIDD"
     gene            12183..12932
                     /locus_tag="PC471_002303"
     CDS             12183..12932
                     /locus_tag="PC471_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005340256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2789430.1"
                     /translation="MNTILKVNNITKTYNKGQQPSLDKVSFNVSEGEFIGIMGASGSG
                     KTTLLNVLSTIDKPTSGDIFINNINLKSLNDTCAADFRKDNLGFIFQEYFLLDSLTIF
                     ENISVPLTLQKLNPDKIENMVKSLAERFGIASQLNKYPNELSGGQRQRASAVRAIVKH
                     PSILFADEPTGALDSNSATELLHSLQEANEELKTTILMVTHDAYVASFANRILIFKDG
                     HIIKELNKQQNNRHTFYELIIQEIAKLDTKR"
     gene            12945..14972
                     /locus_tag="PC471_002304"
     CDS             12945..14972
                     /locus_tag="PC471_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2789431.1"
                     /translation="MNIFYTAIKNLKKNFSFYSLYLLSVSFVITVFFAFTSFSVNKIM
                     LEKISTDGRVESMCNVISIFLMAFVIFYMAYSNRFFLRRRTKELGIYALLGYRKFTIL
                     SLLTFENVLICSGAFIIGLVLGALAHKGIVFGITALLNLSIDNSQIPFFNINAINKTI
                     IFIALVVLVLAVSNGKFFYKTPLVKLIRYEKSAEKKLKFRKIPAFMGFIMILSGYCLS
                     LDILRGAKSVWVSIGFYQMGLITMIIIVIGTVLFINSFLPYIMQNSKKNKRTFYTDMK
                     IIAIPNFIYRIRSNSKTLIMLTLLSATTLTVSSVMALSLYYPIASVSRIAPSEIEFRV
                     EKNKQIEDVKQIVSQYTSNTDDIIFTQTNIYKVKSSSKDLPFEYEIGTSKNNLSNSSS
                     SKEVISRESGFECISFSDYYTLLKAQNRTKLLKQLTPLRDNECILMKYQPNNDGYSEN
                     GTIYPLLVNGKETPVAVRETSLNNAISFANSVGTLIVNDSIYEQIAQNKLPMTRILSI
                     NGKSIKNNENLYSNISDYLDGSPYLQGYSHRINDLLSINSSTFLLIGFLVVLFFIAVG
                     SILYFNNISAISDSTSDYKILQKMGYTNIQIKKIIKKQVLPFFNIPFLLGIVDCIFAT
                     LVYKTALMQNLLGNSLSQYIPVIIAIFLTSVVYLIYYILTIKSCCKIVLKK"
     gene            15116..16699
                     /locus_tag="PC471_002305"
     CDS             15116..16699
                     /locus_tag="PC471_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDF2789432.1"
                     /translation="MTNFNLKILFLGNYILSLLFMGISTSTETYMNIQSIIPLIISTL
                     LLIDITYITSCNVRENNIISLFCGLLALDSWYLLLSFVTTPIGTIIFKILSTVIVYVS
                     IKFIFLFLFQGSGYNFRKITDILLLITCIGAIVGAFISNRTYACMYGIQFVVSIISFF
                     YIIFYHLKRVVFVIKSEKKPISISVFITIIGFLTYYFFTLRISNHIGNFGIYLTVLVF
                     FMSIHGIALKENDSFPISTVFNIKQRAIFLFISILVISLVVLFANGNFSIILIAINVL
                     FMLIFLCNIILEFNLRDEKNKVVKNSKYTFALEKLQQEEQLKTEFSNFLHDDILQNLL
                     SIKNMMSKSYRPDVQDIIYETLNNLNTCIRKQMQDYHPVILKNLTVKENYQNLIESVS
                     SSFPQKNICVSFKCSDTLFLVEPYDILIYRLIKELLTNVYKHSNGSHAWIALALEKNI
                     IKLNISDNGVANTSFKTPLDITKITFDTIKHKGIISIKEQVENLGGTISVSDNIPHGV
                     CVQITIPMKGDGSYKYFVS"
     gene            16671..17291
                     /locus_tag="PC471_002306"
     CDS             16671..17291
                     /locus_tag="PC471_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2789433.1"
                     /translation="MVLINILLVDDHMLFAKSLSIALEEYKEIEQFYSTQDIHNLSEM
                     IQDKKIDIVLMDINLRGLSECDGLKVAYNLLETNSTIKIIILTGYDLPVYKHEAKKIG
                     IYGFLNKNISPDSLIDSLLKVYQGVTCFPSENTEVIIEDLTTMEKRILQLICSGKKRK
                     IIADELYISERTLSNHLQHIYDKLDVSSSVEAITKAIKMGYISPVY"
     gene            complement(17480..18406)
                     /locus_tag="PC471_002307"
     CDS             complement(17480..18406)
                     /locus_tag="PC471_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conserved phage C-terminal domain-containing
                     protein"
                     /protein_id="HDF2789434.1"
                     /translation="MSNSFYQLNSAVLYFNEKRDVNRIRFNHYVMRKENIQRLKGTLP
                     RGQFMMTVRKAASDLDVSISTISRLVNEFIDLGILRLISRGVKGNCCSVYSYVCSESS
                     NAEIVTKNGDIFKNIEKYIDATINNNQKTMKYKHRNELFKNSNTNIYSRINQESIDKD
                     YDLIIHTGYETENETKKKELLKNNLNKKNLNKNTFENSDEDLTEDSTYKNPESIHEII
                     IRKLNSESGKNFKADLSITKALIDKRLKEGYSLEDFYKVIEAKVRRWRYTDMEIYIEP
                     TILFGYEFESYLNEESSLSACNYGDMCYEDMY"
BASE COUNT         6345 a         2915 c         2226 g         7030 t
ORIGIN      
        1 acctttttct ggattagcag agtgagctgg tttaccaaaa gtttctacta ctatttctcc
       61 tctacctctt tgacctatct ttaggtttaa ttctgaagat tcacctatta caacataatc
      121 aggttgaaca gccttactta tctctctagc tgaaacacct tcaaatattt cttcatgaac
      181 aacacctgct acatacaatt caccttcaaa gtctttgtta gtatcttctg caaagtatga
      241 aacagcagcc atcatagcac ttacttgccc cttcatatca gaagttcctc taccatatat
      301 tttcccatct actattctcg cttcaaaagg aggaacactc catttacttg catctggaac
      361 tggaactgta tctatatgcc cgtcaaataa tattttttta cctggtttat ttccttttat
      421 acatcctact atatttccat atctatctct tgaccaacta tcaaaaccta gtaaagcaaa
      481 attttcttct atagctttta ctacgttttc ttcttgacct gaataactag catttcttat
      541 taagtcttgg catacttcag ttaattgttt ttttcttgtt tcatttaaca ttttgatatt
      601 cccccatata atttttaatt tgttgacgtt ttattttgat atttcatttt tttaatttat
      661 tagcatttca cttaatctat tgatttttta tttctttata ttttatttat taaattttta
      721 tttagacttt attttaattt tatttggttt ttattttgaa tttttcattt gctttacttt
      781 tttatttgct ttactttttc atttatttag atttttcatt tgttttagtt tttattttat
      841 ttaaagtttt cacttattta agtttttatt ttccttaaat ttttcattta tttgttaaga
      901 ttttcttttc cttaagtctt ttatttactt tggcattcac cattccaaac tatatctcta
      961 tatttatcag ggtcagtatc tccttctgta cttattaata atactttaga gttctcatct
     1021 agtttcaaag cttctcttaa gtcttttaaa tcttcacttt caaggattga aactaatgct
     1081 ccagttgtaa ctgctccaga ttctccagag attactttct catctcctct tagagggttt
     1141 cctaatattc tcataccttt agcagatacc cagtctggtg ctgaaacgaa tgctgatgaa
     1201 taatttttta acacttcaaa acctatagtg tttggttctc cacaagcaag ccctgccatt
     1261 atagttggca tatctcctcc aacaaatctt ggttttccat ctccagcttc tgctgattta
     1321 tagtaacagt cagcctcatc agcctctact actactgtta ttgggcattc atcatatatt
     1381 gaagcaacat atccttgaac tgcaccagca agtgaaccaa ctcctgcttg aacaaatacg
     1441 tgtgttggtc tatctactcc atattctttt aattgttcta tagcttcttt agccatagtt
     1501 ccatatcctt gcattatcca tgctggtatt tcttcatatc cgtcccatgc tgtatcttga
     1561 accattactc catggtcatc agcttctgca taatcattag ctaatcttac tgcatcatca
     1621 tagtttaagt ctgttatact agcatctgca ccttctcctt tgattttatt taatctaaat
     1681 tcagaagaac cttttggcat taatacaaca gatttttgac ctaatttatt agctgtccaa
     1741 gcaactcctc taccatgatt tccatctgta gctgtaaaga aagttatttc tcctaatttt
     1801 tcttttattt cttttgaagt taatttatca taccctaact cagatatatc tgtatctaat
     1861 ctttgagcta agtatctacc catagagtaa gaaccaccta aaactttaaa tgcgtttaaa
     1921 ccaaatctat aagattcgtc tttaacatat attccgctta cgcctagtga ttttgctaag
     1981 ttatctaatt taactaaagg agttttttca tattgtggaa agctttcatg aaaagcttta
     2041 gcctttgcaa tctcttcatc ttttaaaaat tcaattcctt tttctttttc ctctgattta
     2101 ggtaagttat ttaatttcca ttttatttct ctcatttatt ttatattccc ctttcaaatt
     2161 tttcggtgtc attttaattt tttatctttg tgccttatag caaagcaatt accgtgccaa
     2221 tattatttat tctaaaaaca atttttttat gttggaatta catactttta taaaatttct
     2281 ttaaagttta taaattttga gtgtgaaaaa taagatttat aaaagaaaaa attctcatat
     2341 tgggaaattt ttatcaatac gagaattatc aattcgagaa atcgtttatt tatcttaatt
     2401 cttcctttat tatgctctta ttttttatat gtctattgtt ttgtcagata tattactact
     2461 tttattttct ataaatatat ggctataacc ttcttacgca taaactctat aaatttatat
     2521 actttatatt ctttattacc caaatattct ttattaccca aagcctccaa aatggcttaa
     2581 atcttttcat agcttcttac agaagtttac atgggcgtcc ccccaataag caaaagtgca
     2641 atatgtgtac cattgtgccc tacttgcgac tattgagctg tcgaaattcg caacttcgca
     2701 tttatctgat gaaatgcact atacaaaata ttcataacat ccacaacaga gttaaagtta
     2761 cctattcata ttcactgtaa aaatttcttc cacaggaaat gtaattttaa ccaatcctat
     2821 tgagacaaac tcaccactta tttttttgaa ctcacgcagt aatacatcac ttgcccgaaa
     2881 aaaagcctca ctttcatcat ctccagtaag tgctattgtg caatggggta cccaacgatt
     2941 ggggtggtac cattcccaac catttgaatc aaaatcttgt agtacctcat ataattctct
     3001 ctgaaactgc tacatactgc tattcattat aggtgataca aacaccgttt tagtatcatt
     3061 aaacatgcct actgaaccaa tgtaagcagg tattacctta tggttttgtg caaacccctt
     3121 tagcttgtct atacaacaag cctcatccac atcattgaaa caagccaaag taagatgagg
     3181 tcttgttttc cattctaaat acttcgtact aattttttca tcagcgattt ttttagataa
     3241 atcaaaaagt tgcttttccg tttttttatc ataatacagc tctatcgcat attgcataat
     3301 aacacctcca ccataaagta aaaataccta tctataaaaa cacacaaata cctatcattt
     3361 taatattata ttactactgt tcctcttcca attttctata tagtgtagcc aaacctatgc
     3421 ctaagtcttt tgctatcttc ttcttcgtct ttgtatctct tccatactta ttcaacaact
     3481 tgtttatgta agtcaactct aattctttaa ttgaaagtat cctttcttga ttctctacta
     3541 tccctccaac tatatcatct tcaaatatta tatttacatc cttattttta taaatatttc
     3601 cattaatatt ataatagttt aaaatatcta atggaagcat atctttatat attattccat
     3661 catctccaac caaattcatc atatattcaa ctgcattttc cagttccctt atatttcccg
     3721 gccaattata gttaatcaac atattcatta cattttcttc tattttattt aactgaatac
     3781 ctaattctaa agaatattta gaagcaaact tcatcattat tgcctcaata tcatcctttc
     3841 tttccctaag aggtggcaaa tttataggaa ttacattcaa tctatagtat aaatcatccc
     3901 taaacttacc ttcatttact aatttcaata agtttttatt tgtagcagca atcactctta
     3961 tatctaaatc tataagttta tttgacccaa ttctcgcaaa ttttctctct tgtaaaactc
     4021 taagtaactt tgcttgtaaa ggcattgaca aatctcctat ttcatcaaga aatataacac
     4081 cttcatttgc aagttcaaat ttacctactt tacctccact actagctcca ctaaatgcac
     4141 cttttatata gccaaatagc tcactttcaa ggagattttc tggaatagca gcacagttta
     4201 tcgcaataaa tggcttgttc catctgctac cttcagcatg tatagcccta gcaattagct
     4261 ctttacccgt accactttcc ccagttataa gtacagttga atttgacttt gctaatttct
     4321 tagttctctc ttttactttt aacattgctt ctgaatttcc aattatagac tctatatcac
     4381 tatttcccca gccactattc acttttccat gtcctttatg attgatatat gtcttgtcaa
     4441 atattattat tttgtcgtat tgatttatat atggatatac cggaattatt ttggcattta
     4501 tattataaat tttgttatca atatcaattt ctagtaattc atgtccattt gaggaagatt
     4561 caacagatac tatatttact atattttcta ttatattttt atctatatct aattttttta
     4621 gagctatatt gtttatataa gatattttgc tatttatgtc tagtattata acgccttttt
     4681 caacattttc aatgatttct atcatcatat ccatttttct actgctattt tttgacgctt
     4741 catgttcttc tattttcata gaaattaaat cgcatatatg attgacaaat tttaaataat
     4801 tatctatttt tgcacttatt tctacttttt tagttttatc tgtactaata gcaccaatta
     4861 ctcctatagt tcgattatta tactttattg gaactgctat tattacttta tttaggcatt
     4921 tcatgtaatg ggaacattct atacaaagct gattatcaca tatatccaat acgactaatt
     4981 cctgacctgt ctgtataaca ttgtcataaa taaagccctc agtaacaacg ctttcattta
     5041 tctttgattt atacagacct gtaccactaa ttcttattaa tctatcgtca actatttcaa
     5101 tatctacatc taacacacta gctactgttt cagtgtattt tatcatttct tgttgaaatt
     5161 cttttaattt acttaacata gtttccccca ttagaattta atttaccaaa ttatcagttt
     5221 agctatattt taacatatag tcactcttta aaatatatat aaaacattac ttatttatat
     5281 atttgcgtta aaacagacaa tattaattta taaaaagttt gttttatata gtaaccttaa
     5341 ttataatatg cagacaataa aataaatcac aatacaataa taaaaaaaag ctttcctcat
     5401 ataacaaata tatttttgac ataaaaatat acattatatg agaaaagctc aatagttaaa
     5461 tatttagata gatttttata tagacttttt taatataaac ttttttaatt gtagactttt
     5521 tttattagtt tttaattttt attagttttg ttattggttt tattgttata atacttataa
     5581 tactgccatt gcttcaattt ctaaaactgc atcttttggt aattgagcaa cttgtacaca
     5641 tgaccttgct ggtggatttt ctttaaaata tgtagcatat acttcattaa tatcattaaa
     5701 atcctttaaa tcttttacga atactgtagt cttaaatatt ttatccatac ttgtaccagc
     5761 ttgttctaat attgctttta cattatctaa agaagctttt gttgcttctt ttgggtcaga
     5821 tactaactcg ccagtcttca tatctattgg aagttgacct gatgtagaaa ctatgtttcc
     5881 aacttgaatt gcttgagaat atggtccaat tgctcctgga gctttctctg tagctattac
     5941 tacttttgac atgacttcac ctcctattat taagttatat ttacaggaaa ataaaatttc
     6001 ctaaacacca atattaatac gaacctttag attaaacttc aaatctaaaa cttcaaataa
     6061 tcaatactta gatgattatg tttatttgtg tcttgttaac ttttattaaa gcaattaacg
     6121 tgccaaactt tatttatata taaaattttg tgaagtcatt caaaatagtt acttctgaat
     6181 tttttcgtaa aaaaacgctc taaaattact tttttatgat gtcaattttt ttctcatatc
     6241 tagaaattta ccaacatgag aattcttgtt ttgagaattt acagattaaa gttatttttt
     6301 aaccttaaat tctgaacctc ccatgactaa agtcacaggg ttcctgcttc atagaatttt
     6361 gcataatcaa gttattacat atgaaagaac tatagttgac aatcccacta ttataaataa
     6421 taaaaccttt acccatacct tatttaccct agttatcata gagcctattg aaaatataac
     6481 agctaaatat aataataacc aaaaaattat caatatctac cactgttgct gaaaatccaa
     6541 aaaactacct tttgatagta ttacttaaca atctaattaa tctcataata aatttacaac
     6601 ttataaataa attgttatat tggccgtatc tcaacaactt tctcctttcc tagttttaaa
     6661 tcttttatag aattgtacca cgtctaagta aattttcata ctattaagtc ttatttctaa
     6721 taaatcatta gctctcattc tatgcccaat aatgtaagat tgcagtaatt ttattgattt
     6781 taatagtttt atttaatata cttatattaa ttattttcat atgaaattat tatttatata
     6841 gggggatttt tttaatatga gcaaaagtga attaacagca gaaacaacag aagaaatgtt
     6901 agaagtacta agtggtaaag attatgatat tgcatgtcat ttacatgaac ttggtaaatc
     6961 attagattgt aaaattgaac caaaaacagg tgctcgttct tacaaaatag tatattcaac
     7021 taagaaacca aaacgtagct tatttactat tgaatgtaat gaaaaaaaat ggagagttaa
     7081 agcaaatctt tttcatctaa atacatataa agatgctgtg gaggaatgct ctaaaactat
     7141 taaagatagt ataactaaaa ctcgtacttg taagaaatgt aattcaaagt gtattggagg
     7201 ttcttcgttt gaattagatg gaaagtctta cctgacttgc ataggaagtg gtcattattt
     7261 tgcaaatatg gaagaaatgg attggaaaaa cctagaaaaa ttaattacta aagaaaataa
     7321 tattatgcag gaatctgtat agttttttaa gggtaattat taaaatatat gagtaacaac
     7381 agatacttaa ataggcataa aaaaataaag ggtataataa ggatatagaa taaaaataaa
     7441 tgatatagca caaagaggct gtctcaaaat acagataagc ctctttttat tgttgaaatg
     7501 ataagaatat attcaatttt atacaatttt atacaaacat actacgccaa cttacccatt
     7561 tcatatgcct gtttcattgc cttacttccc ttaatatcac ctacattcca agcacctaca
     7621 ccatatacta tacctttttc cttagaacca tttagacatg atgtaaatcc tctaaatcct
     7681 tctattgttc tttccatatc agccacattt tcatctgctg ctgtaacgat aaaatagaaa
     7741 tctttatcac aaatttcagt atatctagag caagttctat caatcatagt tttcatttgc
     7801 gcattcatag tataaaaata aactggagtc gctaaaacaa tcacatctgc ctcaatcatt
     7861 ttttcaagaa tttctcccat gtcatctgtc tgtgaacaat ttgcttcttt ttcataacaa
     7921 gcaccacatc cagtacaata gttaatatct ttatctttta aaaatatctt ctccacttga
     7981 tttttagatt ctaatacccc ttgtataaac tggtcacaca ataaatcaga atttccacct
     8041 tttctaggac tagctgatac tattaataca tttttactca taagatactg cctccatttt
     8101 cttatttcta ctgctcttta atactttttc tttctcaata ataccttctt catatctatc
     8161 aatcttaaaa tttaatcgtt ccaaagtttt ttgcatttct tctattcttt ttgctagttg
     8221 gtttctctgc tctattaaaa gttcttttct agctttgata gtactgctcc cttgttgaaa
     8281 catagctaca tactcaatta atgtctcaat agaaagacct gcacctctca tacactttat
     8341 gaaattaacc cactcacaat cttcttctgt aaaagaccta attccacttt tatttctatt
     8401 tacaggtggt attaaaccaa ttcgttcata atatcgtaaa gtatccgctg aaagctcgta
     8461 ttttttacta acttctgaaa tcgtcataca tatcttctcc tcttgatatt gtatagattg
     8521 aattatttct caaaggtttc tgctacattc tttaaagcat ctataatttt aatgtgttga
     8581 ggacagcttt cttcacattg tttacattca atacaatctg aggcctttcc ataagtttta
     8641 gtataattat tatagtagac catctgtgtt gaaaatccag tattaagagc ttgtttttct
     8701 gcattgtaca acgcaaaata gtttggaata gcgatatttt ttggacagcc ttctacacaa
     8761 tactgacagg cagtacaagg aactaaaatt gattcgttga taatctctac tgcttcatct
     8821 ataatattat attcctcttg tacgaatggt ttgaaatttt gcatatatcc tgtattgtct
     8881 aataattgtt ccatatttga catcccactt aagaccatca taacattatc tttacttgct
     8941 gcaaaacgta ttgcccaaga tgaaattgac atatctggat tgtagtcatt caataatttc
     9001 tctgcttttt ctggtatctt tgcaagtgta cctcctttta ctggctccat aacaattaca
     9061 ggcttattat gcttctctgc cacctcgtag cattttctag attgtatgct ttcattatcc
     9121 caatctaaat aattaatctg taattgcacg aagtctactt ctggatgctc actcaagact
     9181 ttatccaata attctgcact atcatgaaaa gaaaaaccaa tattctttat ttttccttct
     9241 tttttctttt cttcaataaa cttaaagcta tcaaattttt tagctacttc ataatgtgac
     9301 acgcctatat tgtgtaaaag atagtagtca aaatagtcta caccacattt ttctaattgc
     9361 tcattaaaaa tggtttcttg ctcttctttt ttctttaaag ccattaatgg aagttttgta
     9421 gctatagtaa aactctctct tttgtgtctt tttactagag attctctaag tgctatctca
     9481 ctttttccca tgtgatacac ataggctgta tcaaaatatg taaatcccct ttctaaaaaa
     9541 gtgtctacca ttttatttaa atgttccata tcaatagttg taacatcatt ttcatctttt
     9601 attggtaaac gcattaatcc aaatcctaat ttttttgcct ccattgtttt acttccccct
     9661 ttaaaacttg tttgtttttt ctctctactt taactattct tacaagtata taattcttgt
     9721 ataattatac tttactactt ggagttaact tcaagtcaat agattttttt atttttaaga
     9781 caaatattat aaaaatacag ttctttaata ttaatccatt acatgtttct ttcaccctcc
     9841 aattctcaaa cttcacaaac tccaacatgt aaagaaatat atactttaag aaattcagtc
     9901 cattaatatt actacaaata ctttttagat atgtttaaaa agaaatttca cctaaaagat
     9961 ttagaaatag gtcttgtgtc tattttaaaa tatataaaac agtggtaaag acttatttat
    10021 aagataaaaa atgaatagtg tatacccttt ttaaaaagtt ttttcctttt tatatttgtg
    10081 atacaatgtt aaatataaaa aggaaaattt tgtattaaaa aaatcttatt tgttgatttt
    10141 gtaacttcaa actcagcatc aatttgattt taagattata ttttattaag tatgctaaat
    10201 aacaaatttt taaagattgt gaggtaaaaa tatgaataag aaaaatttag ctatattaat
    10261 atcaggtatt atgttttttt ctagttcttc agcaattttt gcagacaata ctacaaagca
    10321 agaaagatta ataggtaaaa caagatatga aacagcagta gaagtaagta aattgggatg
    10381 ggttcaatca aaaacagcca ttatagttaa tggtaattct attcaaagtg cgctttgtgc
    10441 aaatccattt gctaagctaa agaatgcacc tatactttta gtaaacaaca acagtattga
    10501 aaattcaact aaggctgagc ttaaaaggct tggtgtagat aatgtgtaca ttgtagatag
    10561 tggaaatagc atttcttcta aagtagaaaa tgaaataaaa tcacttaaca taaaaataaa
    10621 taaaatagta ggaaacaata tatatgaaat gtctacaaat gtacttaaag aaatagataa
    10681 gataaaaaag gtagaaaatg ttgcagttgt aaatggacta aagggactat ctgatgcagt
    10741 tagtatagct tctccagcag caatacataa tatgcctata atattagtat cagatgaaaa
    10801 cagcaatgat agtagtataa atttcttaaa caataggtct ataaaaaaat cttacatagt
    10861 tggaggaaat aagattttat cagacaatac agtaaagaaa tatccaaaca gagaaagaat
    10921 cagtggttca gatagaaaca atactaatgc aaaaattatt gataaatttt ataaagataa
    10981 aaaaataaat aatgtatttg tagctaaaga tggtattaac caagaaaatg cattagtaga
    11041 ttcattatca ataggagtac tagctgctaa agaaaattct cctataatga tatctaatgg
    11101 tagccttgta aatagtcaaa aagaatttat taaaaataaa gatatagatg aaataacaca
    11161 agtaggagga ggatataatc aaaaagcatt tgaagatatt ttaaaactaa aaaatgtgaa
    11221 cataagaaat aaaactaaaa aaatatcttt aatacaggct aaagaaatat taaaacaagc
    11281 tcttccagga agcaatattt tagtttgtta ttatgatgaa gatgataatg aatatgactg
    11341 caaagttata aaggataata tcctatatga gatagaaatt gatgcctact ctggtaaaat
    11401 aatagaaatt gaaaaagatg atgacttaaa tgataataat ttaggtacac aaaatatatc
    11461 attggataaa gctaagtcta tagcattaga tgctgttcct aatggtaatt taatctattg
    11521 taaatatgat gctgaagata acgaatatga ggctaaagta ataaaaaata agaaaacata
    11581 tgaattagag ataagtgcat ttgatggaag aattgtagat attgacatgg acaatgattc
    11641 tgatgatgac tttgatgata atgataagaa taactctaat actaatgaca acagttctat
    11701 tattagtgct gaaaaagcta aatcaataat gctagataaa gtgcctggtg ggaaaatagt
    11761 aaaatttaag tatgatgaag atgacaaaga atatgaaggt gaaataataa aaggaaaaac
    11821 tgaatatgag ataacaatta atgcaattac tggtaaaata ttagaatttg aaatagatga
    11881 ttaataaaaa atatatattc tattaattat tacaagcaat aaagccataa atattgaaaa
    11941 tttcaatatt tatggcttta ttttattttt tttaatttaa atccacatta tgccattata
    12001 tatacctatg cactctttta aattaaaatc ttgtccatca tcccaaacct aataaagtta
    12061 gattttaaac aatatattgt gatataagta atttgtgtag aattattgcg tgatttactc
    12121 atgaagaaac aataaggtta atatataatt tgactatatt aagacgaaag gagacatata
    12181 caatgaatac aatattaaaa gtaaataaca taactaaaac ttataataaa gggcaacagc
    12241 cgagtttaga taaggtatcc tttaatgtat cagaaggtga atttataggt ataatgggag
    12301 cttctggctc tgggaaaaca actctactta atgtattatc tacaattgat aaaccaacaa
    12361 gtggagatat tttcataaat aatataaatt tgaaatcgct taatgacact tgtgctgctg
    12421 attttcgtaa ggataattta ggctttatct ttcaagagta ttttttacta gatagtctta
    12481 caattttcga gaatatttca gtacctctta cacttcagaa actcaatcca gataaaatag
    12541 aaaacatggt aaagagtcta gctgaacgat ttggcatagc ttctcaatta aataaatacc
    12601 caaatgaatt atctggtggg caaagacaga gagcttcagc agttagagca atagtaaaac
    12661 atcctagtat tctctttgca gatgaaccaa caggagcttt ggattctaat tcagctactg
    12721 aactactcca ctcactacag gaagcaaatg aggagttgaa aacaacaatt ttaatggtaa
    12781 cacatgatgc ctatgtagca agttttgcaa atagaatttt gatttttaaa gatggtcata
    12841 tcattaaaga attaaataaa caacagaata atagacatac attttatgag ttgattattc
    12901 aagaaattgc aaaattggat actaaaagat aggaggataa tttaatgaac attttttata
    12961 cggcaataaa gaatcttaaa aaaaactttt cattctattc tctatatcta ctttcagtct
    13021 cttttgttat tactgtattt ttcgcattta cttcattttc tgttaataag ataatgctag
    13081 aaaaaatttc aactgatggt agagttgaat ctatgtgtaa tgttatttcg atatttttga
    13141 tggcatttgt catcttctat atggcatact caaatcgatt cttcttgaga cgtcgtacaa
    13201 aagaattagg tatatatgct ttacttggct atcgtaagtt tacaatatta tcgctactta
    13261 cctttgaaaa tgtattaatt tgtagtggag cttttattat tggattggta ttaggagcat
    13321 tggcacataa gggtattgtc tttggaatta cagcactact taatttatca attgataatt
    13381 cacagatacc attttttaat ataaatgcaa taaataaaac aattatcttt atagcattag
    13441 ttgttttagt cttagctgta tcaaatggta aattttttta taagactcca cttgtaaaat
    13501 tgataagata tgaaaaaagt gcagaaaaaa aattaaagtt cagaaaaatt ccagccttta
    13561 tggggtttat tatgatttta agcgggtatt gtttatcact agatattctt cgtggagcta
    13621 aatcagtatg ggtaagtatt ggattttatc aaatgggctt gataactatg attatcattg
    13681 taattggtac agttctcttt atcaattcct ttcttccata tattatgcaa aatagtaaaa
    13741 aaaataaacg tactttctac acagatatga agataatagc tattccaaac tttatctatc
    13801 gtatacgttc aaactctaaa actctaatta tgcttacatt actctctgct actactttga
    13861 cagtttcaag tgttatggca ctgtccttat actaccctat tgcatctgtt tcgcgtattg
    13921 caccttcaga gatagaattt agagtagaaa aaaataaaca aatagaagat gtaaaacaaa
    13981 ttgtcagtca atatacgtct aatacagatg atattatttt tacacagaca aatatttata
    14041 aggtaaaatc ttcatcaaaa gaccttccat ttgaatatga aattggtaca tctaagaata
    14101 atttatccaa tagtagttct tctaaagaag ttatttcaag agaatcagga tttgaatgta
    14161 tatctttttc tgattattat actttattaa aggcacaaaa tcgaacaaag ctactaaaac
    14221 aacttacccc tttaagggat aatgaatgta ttttaatgaa atatcaacca aataatgatg
    14281 gatatagtga aaatggaacg atttatccat tacttgttaa tggtaaagaa acacctgtag
    14341 ctgtaaggga aacatcatta aacaatgcta tttcttttgc caatagcgtt ggaacactta
    14401 tcgttaatga tagtatatat gagcaaatag cacaaaataa attaccaatg acaagaattt
    14461 taagtattaa tggtaaatca attaaaaata atgaaaattt atatagtaat atttcagatt
    14521 atttagatgg aagcccttat ttacaaggat attctcacag gataaatgat ttattatcaa
    14581 ttaatagttc tacatttctg ttaatcggat ttcttgtggt attattcttt attgctgttg
    14641 gaagtatttt atactttaac aatatatcag caatttcaga ttcaacatct gattataaaa
    14701 ttttacagaa aatgggttat actaatatac aaattaaaaa aattattaaa aaacaagtat
    14761 tgccattttt taatattccg tttttacttg gtatagtgga ctgtatattt gctaccttgg
    14821 tttataagac tgctttgatg cagaatttac ttggaaattc actttcacaa tacatacctg
    14881 tgattattgc aattttctta acttctgtag tatatttgat atattacata cttactatta
    14941 aatcctgttg taaaattgta ttaaaaaaat aatatttgcc aaatcgtcaa gcttaaaggt
    15001 gtgacgattt ggtatacttc atttataagt atttgatata atttaaaact ataaattctt
    15061 aagtttgatt tagtcataaa attaatataa ttaactcata aggaggtgta taagattgac
    15121 aaattttaat ttaaaaatac tatttttagg aaactacatt ctatcacttc tcttcatggg
    15181 aatatctaca agcactgaaa cttatatgaa tattcaaagc attattccct taataatttc
    15241 aacattatta ctcatagaca tcacatatat aacctcttgt aatgtccgag aaaataacat
    15301 tatttcattg ttttgtggat tactagcact agacagttgg tatttactct tatcatttgt
    15361 aaccacacca ataggcacta ttatttttaa aatattaagc acagttatag tgtatgtctc
    15421 tattaaattt atatttttat ttttgtttca aggtagtgga tacaatttcc gtaagataac
    15481 agacatttta ttgcttatca cctgcattgg tgcaattgta ggagcattta tttctaacag
    15541 aacgtatgct tgtatgtacg gaatacagtt tgttgttagt ataataagct ttttctatat
    15601 tattttttat catttgaaaa gggttgtttt tgtcataaaa agcgaaaaaa aacctatatc
    15661 gatttcagta tttataacaa ttataggatt ccttacttat tattttttta cactaagaat
    15721 atcaaatcat attggtaatt ttggaatcta tctaactgta ctggtatttt ttatgagtat
    15781 tcatggtatt gcacttaaag aaaatgatag ttttcctatt tcaacagttt ttaacataaa
    15841 acaaagggct atatttcttt ttataagtat tttagtaatt agcttggtcg tactatttgc
    15901 caatgggaat ttttctataa tattaatagc tataaatgtt ttatttatgc tcattttcct
    15961 ctgtaacatt attttggagt ttaaccttag agatgaaaaa aataaagtag ttaaaaatag
    16021 caagtatact tttgcattag aaaaattaca acaagaagaa caactaaaaa cagagttttc
    16081 taatttttta catgatgaca tattacagaa cctgctttca ataaaaaaca tgatgagtaa
    16141 gtcatatcgc ccagatgttc aagatattat atatgaaaca cttaacaatc taaatacatg
    16201 tataaggaag caaatgcaag actatcatcc tgttatttta aaaaatttaa cagtaaaaga
    16261 aaattatcag aatttaattg aaagtgtttc ttcatccttt ccacaaaaaa atatttgtgt
    16321 ttcatttaag tgttctgata ctttattctt agttgaacca tatgatatat tgatatatcg
    16381 actgattaaa gaattattga caaatgtgta taaacattca aatggtagcc atgcatggat
    16441 agctttagca ctagaaaaaa atataataaa attaaatatt agtgataatg gtgtggcaaa
    16501 tacatctttt aaaacgcctt tagatataac taaaattact tttgatacaa taaaacacaa
    16561 agggattata tcaattaaag aacaagtaga aaatctagga ggaacgataa gtgtttctga
    16621 taatatacct catggagttt gtgtacagat tacaatccct atgaaaggag atggttctta
    16681 taaatatttt gttagttgat gaccatatgc tttttgctaa aagcttgtca attgcactag
    16741 aagaatacaa agaaatcgaa cagttttatt ccacacagga catccataat ctttcagaaa
    16801 tgattcaaga taaaaaaata gacattgtct taatggatat aaaccttaga ggcttaagtg
    16861 aatgtgatgg cttaaaagta gcttataatt tattagagac taattcaact attaaaataa
    16921 taatcttgac tggatacgac ctcccagttt ataaacatga ggctaaaaaa ataggtatct
    16981 atggattcct caataagaat attagccctg atagtttaat tgattcatta ttaaaagtct
    17041 atcaaggggt aacttgtttt ccatctgaaa atactgaagt aattattgaa gatttaacta
    17101 ctatggagaa aagaatttta cagcttatct gtagtggtaa aaaaagaaaa ataattgctg
    17161 atgaattgta tataagtgaa agaacattat caaatcattt acaacacata tatgacaagc
    17221 tagatgtttc atcatcagtt gaggctatta caaaagctat taaaatgggt tatatttctc
    17281 cagtatatta aaaaaagtga gtgtctcaaa ataaatcttc taactcataa agcactctcc
    17341 tttgtattaa atcaaatata ttttcatcat ttaaacaatt aagaagaggc ttctctctat
    17401 tttgagacaa ctccatttta atacactatt tatatttata tataaacata ttttaaacaa
    17461 ctaaaaagca actaatatct caatacatat cttcataaca catatcacca taattacaag
    17521 cagataaaga actctcctca tttaaatacg actcaaattc atacccaaac agaattgttg
    17581 gttctatata tatttccata tctgtatatc tccatcttct tacctttgcc tctataacct
    17641 tgtaaaaatc ttctaaacta tacccttctt ttaatctttt atctataagt gccttagtta
    17701 tactcaaatc agctttaaaa ttttttcctg attcactatt taattttctt ataataattt
    17761 catgtatgct ttctggattt ttgtaagtag aatcttcagt caaatcttca tccgaatttt
    17821 caaaggtatt tttatttaaa ttctttttat ttaaattatt ttttaataat tcttttttct
    17881 ttgtctcatt ttctgtttca tacccagtat gaattatcaa atcataatct ttgtctatac
    17941 tctcttgatt tatacggctg tatatgttgg tattactgtt cttaaacaac tcatttctat
    18001 gtttatattt cattgttttt tggttattgt taatagttgc atcaatgtat ttctctatat
    18061 ttttgaaaat atcaccattc tttgttacaa tttctgcatt actactttca gaacaaacat
    18121 aactatatac agaacaacaa tttcccttta ctcccctact tattaatctc aatataccta
    18181 aatctataaa ttcatttaca agtctactta ttgtagatat agatacatca aggtcagatg
    18241 cagctttcct tactgtcatc ataaattgtc ctcttggcag agttcctttt aatctttgta
    18301 tattttcttt tctcattaca tagtgattaa accttattct atttacatct cttttttcat
    18361 taaaatataa tactgctgaa tttaattgat aaaaactatt actcatattc ccactcctcc
    18421 aatttaaata gataatttgt tgtaaagttt ttagattttt aaataactta ttaaatgatt
    18481 atttttatta aaaagtattc tttgatattt tcattt
//
LOCUS       DAMJYR010000067        18448 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.067, whole genome shotgun sequence.
ACCESSION   DAMJYR010000067 DAMJYR010000000
VERSION     DAMJYR010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 18448)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18448)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18448
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.067"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            <1..866
                     /locus_tag="PC471_002308"
     CDS             <1..866
                     /locus_tag="PC471_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF2789435.1"
                     /translation="VFFVIDYINFRNSYTNLYNCIENSGEIDSYLVDGQSFEENLIKD
                     IIENLKIKNNSDIETYKQSLKELDEYIAKWVHEIKIPISSLSIITDRLSSIEDSLDIK
                     NQVAKINFLVNSILYSSRSNTMFEDVFINKFNLEKLIKMSIKNNSFLLIKNNVEVSLN
                     ELENDVYTDSKCMSYVLDQIINNAIKYSKEVGKIEFNSKKLENGVVLSIKDFGIGINE
                     EDISRVFDKGFTGKNGRNQLYKSTGMGMYFVKKMIDSLGHEIEVCSENGSYTIFNIYF
                     YDISDYLSLDS"
     gene            1297..2457
                     /locus_tag="PC471_002309"
     CDS             1297..2457
                     /locus_tag="PC471_002309"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HDF2789436.1"
                     /translation="MITMYSVGLMSGTSLDGVDAVLAEISGNGRNTKVKQIEFITLEI
                     PKDIKDEIRKCCIEEESSVDLICSLNFKLGYLFSKAVKSVCHKANFHIANLDFIASHG
                     QTIFHIPRSYNNFVSSTLQIGEPAVIAYETNTKVISNFRVMDIAAGGEGAPLVPYSEF
                     LLYSDNNKNLALQNIGGIGNITIIPKSCNIDDVFAFDTGPGNMIIDGVCQRLFNRKYD
                     KNGYFASKGKINEEMLKDLMSHKYLSQAPPKSTGREVFGQVYLDNMLNKYKHVDKYDL
                     IATVTMFTAKAIYYNYKNFILPKVNVDTLLIGGGGAHNLTLIGYIKELLLEVEVLTQD
                     EYGYSSDAKEALAFVILGNETLNNSFSNVISATGAKNKVILGNITPKPFGGK"
     gene            2459..2857
                     /locus_tag="PC471_002310"
     CDS             2459..2857
                     /locus_tag="PC471_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2789437.1"
                     /translation="MSNNKKKKRKKNNNYKNAKSTKSNKNTKKQMEKNQRTDYNIPTI
                     SFKSGLILIVSSLVSTVVFPLLLSIFGIDNRFGVVIGNSLITSFAVAYTRYFVETKRG
                     FSLGFFRLYILFALSFAIIGYFWIYKGIHI"
     gene            3120..3428
                     /locus_tag="PC471_002311"
     CDS             3120..3428
                     /locus_tag="PC471_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDF2789438.1"
                     /translation="MRVLFVCSSGMSSAIAVNALQKEGAKNGIDIDVLAVGTQEFEDE
                     VKNGWDIAMVAPQVRHRFDYLKAFADEVSVPCALIQAQAYSPLGGPKLLKQVQELLSK
                     "
     gene            3517..4863
                     /locus_tag="PC471_002312"
     CDS             3517..4863
                     /locus_tag="PC471_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDF2789439.1"
                     /translation="MLDKFTEFLDTKLSTSMARLAEQRHLRAVRDGIVASLPLIIVGS
                     FFLILAFPPLPDSWGVTQYLKENAVTILLPYRMTFFIISVYSTFGIGYSLAKSYKLDG
                     LTGGLLATATFLLTIVPTAIEGLTPDLLKIVEKSPELLAWYNSIPQGWQMPMANLGGG
                     SMFVAIVTSIIGVEIYRFTDKSKFKITMPEAVPASVTRSFEALTPAALIIILMAIVTY
                     FLGFDWHGFIAKLVTPLVSATDTLPSVIILIFLITFFWSFGIHGVSIIGSLARPLWIQ
                     LLDANTASYAAGQAVPGIAAEPFFQWFIWIGGSGATIGLALLLAFRAKSAYGKSLGKA
                     TVIPAIFNINEPIIFGAPIVLNPILIIPFVVAPIVMATVSWFAMSANLVSRVVVTAPW
                     TLPGPVGAYLATGGDWRASVLNLLCIAISIIIYYPFVKMYDKRLLHEEKVEEQEAY"
     gene            5096..6193
                     /locus_tag="PC471_002313"
     CDS             5096..6193
                     /locus_tag="PC471_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HDF2789440.1"
                     /translation="MGRLGISIYPEKTTSKEIYDYIDKAAENGFTRIFSCLLSVKDTK
                     ENIIKKFKSINEYAKFKGFEVILDVNPRVFGELEISYKDLNFFKKTKADGIRLDMGFT
                     GSEEALMTFNPQNLKIEINMSNNTNYIDTIMDYQPNKENLIGCHNFYPHRYSGLNLEH
                     FIKCTERFNKYGLRTAVFVTSQNETTFGPWPVTDGLPTIEMHRNLPIDIQVKHIIAMN
                     NINDIIISNCYPTYEELKVLGSMRKDMVTFDVAIEENIREVEKKILFEEIHFNRGDIS
                     DSLIRSTQSRVKYKGYKFELFNTPKIIKRGDIVIESSEYGHYAGELQIALTDMKNSGK
                     SNVVGYIRKEELFILDYIKPWQKFNFKPSTK"
     gene            6292..7206
                     /gene="murQ"
                     /locus_tag="PC471_002314"
     CDS             6292..7206
                     /gene="murQ"
                     /locus_tag="PC471_002314"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HDF2789441.1"
                     /translation="MLIKTLENLVTEGRNKNTLQIDKEDTLGIIELINNEDKTVAYAV
                     EEQKESIAKAVNIIVDRMKQGGRLFYIGAGTSGRIGILDATECPPTYGVDFELVQAII
                     AGGNQAIFKAIEGAEDDKELGKQDIIDRGVTSKDVICGIAASGRTPYVIGAMEYAKEL
                     GCAVLSITMNPNSEMSKKADLPINIIVGAEVIMGSTRMKSGTAQKMVCNMLTTASMVK
                     MGKVYSNLMVDVKTSNEKLVERAKRIIMIATNVKYDVAEKFLEEADNSVKLAIFMIKS
                     GLDKDSAKSILDRQEGYISEALKSIEKL"
     gene            7316..8236
                     /locus_tag="PC471_002315"
     CDS             7316..8236
                     /locus_tag="PC471_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA reductase"
                     /protein_id="HDF2789442.1"
                     /translation="MYFLGVDGGGTKTTFTLVDEELNIVGTITKGTCHYNQIGFDNLT
                     KLLITGLEEVCKDAKINVEEITYAFVGLAGYGKIKEVLYALEVATKNAYSHINYTLGN
                     DVEIALAGSLNGEKGINIIAGTGSIAQALDKDGNLHRCGGWGYVLGDEGSAYYIGMAT
                     LKMFTMQSDGRCSKTKLYDLIKRHLNIENDYDIIKYVNDEIKGDRIEIAKFATICLKA
                     VIEGDNTASKIFDDAAYELSRLIIGLEHHFEQGTKIKVSYSGGVFKSGDLILEPLKKY
                     LNKTRFGIVRPVLTPDLGACLLAKKKYYSK"
     gene            8253..9104
                     /locus_tag="PC471_002316"
     CDS             8253..9104
                     /locus_tag="PC471_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDF2789443.1"
                     /translation="MSCILKLKQIYEDLTEVDKKIADYILNNTEAISKLSVSELASNS
                     KTSTASIVRFSRKMGYSGFGDLKIEIAKDLMGKENEYTYVDENYDCNIDSVINKITNK
                     NIETINQTRLLNEKDIIKEAVEQIIKAKNVYIFGVGGSALVALDLQMKLLRINKQAFT
                     SLDSHTQLMVSSNVDKEDIAIAISYSGESKEVIKSIENAKLKGCKVICITKYSDNHLS
                     KISDLKLVVPNIEKRLREGAISSRIAMLTLIDIIYISIIQENLNEAEQKLQETKRILD
                     YLNINNA"
     gene            9716..10777
                     /locus_tag="PC471_002317"
     CDS             9716..10777
                     /locus_tag="PC471_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HDF2789444.1"
                     /translation="MKKIRKLATKLLITTIVLISGMSMTVYGMTAKEVTAKTPKSYVT
                     GTNSVYGPKLSQAQLNSVAQATADFMNKKITKNMTTDAKILVAYNHIKNNTTYVDWNA
                     VEGANTAYTLVTKKGACSGMARSMKALCDAMGIESYYVHSTSNDHQWNLIRFGDGRLY
                     HVDIDANKSAGKDIIYKSLSHPLPFDKTAYPKVGAIVQTVKPEQTQKPAPPGKHTMLP
                     HQEGAKAVGNFGKKGEDINEKHVMYEGVRCSVVNSSVNTITFTGSESNLDPSNLTIAV
                     IGFMNTPYGMFALAPEGKPVEYPFKYDKPFSLDFSGKNRSGAELAKYCKKNDVTIKIE
                     ITDEKANIGLGQIMYVRYE"
     gene            complement(10893..11867)
                     /locus_tag="PC471_002318"
     CDS             complement(10893..11867)
                     /locus_tag="PC471_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2789445.1"
                     /translation="MNNINSLKVIKLSLGCSLAIFIAWLLKLEYSMVAGVIVLLTVKD
                     TKRETLKGSIGKIYGFLLCTIFSYLCFNILGYNLTSFSIFIFIIIPLCFLLNIQDVIA
                     MCVVIASHYYLQGETSIKWILNEAGIFAVGTGIGVLINMYIPTNIHKIHEVQKKLQEE
                     VSIVLIDIADIIVNPKKENGYSRDLYTLNSLIDSSISETYDNINNTLLSDTRFFLEHM
                     DIIKSQRDILENLYSYVSQLNSTPPQAHILSAFIHKIGYTEFEAETGNLLLEELKRLM
                     LSMKNQPLPVDRTEFENRAILFLCLTELKQFLVNRKHAQMLRDNNFYK"
     gene            12323..13204
                     /locus_tag="PC471_002319"
     CDS             12323..13204
                     /locus_tag="PC471_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline iminopeptidase-family hydrolase"
                     /protein_id="HDF2789446.1"
                     /translation="MKITEGYMPFKGFKTYYRIVGENTEGKKPLVLLHGGPGSTHNYF
                     EVLDKIAESGRQVIMYDQIGCGNSFVEGHPELFNADTWIEELIELRKHLGLDEIHLLG
                     QSWGGMQAIWYAIEYKPKGIKSYILSSTLSSAKLWEKEQKRRISYMSEVDQKALLDAV
                     NTGDYSSKEYNDALERFMEMYCAGEVTEDSPECLRRPKKSGSEAYIVGWGQNEFSPTG
                     TLSGYEFTDRLHEIKEPCLVTSGAIDLCSPYIAKTMYDRIPNSKWELFEYSRHMPFVE
                     ENEKYIKVLTEWLNAND"
     gene            13586..14389
                     /locus_tag="PC471_002320"
     CDS             13586..14389
                     /locus_tag="PC471_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2789447.1"
                     /translation="MEKLRVIEMIKNNPNIIATIDNPTDEMKLLAIKENGLVLEYINN
                     PTREMQELAIDNNIRAIKFIDNPTEDMMIKAVNEGWSILDYIKNPTDKVIEMAINQAG
                     WAIKYAKNPSEELQLLAVRKNYDSIKFIKQPYESVQEEAVRISYDALRYIDSPSYNAE
                     LIAIKNNEAAISFITDLDKNKMLEFLKVNILVIKYILKKVSKDELESALKEVLSKEDV
                     EEKYIRDFLNCSVIDRNSESIPIDKIMFIYKYGSKTAKKIAVDEKLKIM"
     gene            14401..15531
                     /locus_tag="PC471_002321"
     CDS             14401..15531
                     /locus_tag="PC471_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDF2789448.1"
                     /translation="MNFIDTLKSVDLILSTGEVPLIVGESGIGKTALANKLAKENDWS
                     LIVIDGNLLKEGEIGGLPTIESYVGVNSNGYKTEKKTTVYAVHNKLREIDEEISKSKT
                     VLLFIDEINRCEHTVQQELMNLILNREINGYKLHDDVKILAAMNPSSKYGSDFDYQVV
                     DMDAAQENRFVWLNMESDHTQWIKWAIDEGIERKVIEFISTFPEYLHKINEDDVRATP
                     RSYERVSKIYKVYKEKNNSIPRAVFLNVVKGNVGKVIAEEFISFIESNSEPLISYEDV
                     FLGESIDESIVERVKNESHTRLYLSAMNILKDLELNIKNDKYESNHYINRFIEFLKMY
                     PVDLMIGIMKDIRNSYIEVYKKAIENEEFVKSYFESYSLIRG"
     gene            15535..16944
                     /locus_tag="PC471_002322"
     CDS             15535..16944
                     /locus_tag="PC471_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HDF2789449.1"
                     /translation="MENYFDKQAKELYNKASEIINTHSMLKANRANEKFDIDIPQDFK
                     YEFFSLVDKVNLSLMEEEDNFYGYFLFQMSREIRFDISSPTGVNFKGAKYIIYFNPII
                     FLTLDIKQMETTIKHEIHHILSMHLMRAKELKGKYSTLAINMAMDIVVNKFLNNLPPY
                     ATTLEWVNLKYSLKLEPYEPFEYYVEKIQTELDLMEVDEDGEEDDSDKYGDIEIDYSP
                     ERTHDIWDESNEIDENTLREFTEKFINSSQKGKVPAYLESMISSLKNSKGELPWNLYL
                     NRIMGTVENNKKKTITRRNRRQPNRLDLRGELRSHKSEIAVALDVSGSISDEEFKQAI
                     KEVLSIVKNYNHEITIIECDNEIRRVYKVRTEKDIKDRIRIGGGTRFSPVFEYANNKK
                     INLLVYFTDGKGENKLQVIPRGYKILWVISGRGDKISLKEPYGAVKKLTKVKTNDEIL
                     EINDVRNDGYSMNSQAPIL"
     gene            17155..17664
                     /locus_tag="PC471_002323"
     CDS             17155..17664
                     /locus_tag="PC471_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HDF2789450.1"
                     /translation="MVMFLFKIDDYIMYGMTGVCKVMDITNERFTNGIKKEYYVLSPI
                     YSNNTVIKIPVDNEKVPMRKLLSKINVLSLINDIPNMDTSWIDNEKLRSEQFKKILRG
                     GKCEELIKLVRSIDNNREYVKSIGKKTHQADDNIMKEAERLLNEEFATILDISPNEVS
                     SYISSHIPQ"
BASE COUNT         7376 a         1883 c         3228 g         5961 t
ORIGIN      
        1 tggtattttt tgttatagat tatataaatt ttagaaatag ttacacaaat ttatataatt
       61 gtatagaaaa tagtggtgaa atagacagtt atttagttga tggacaatct tttgaagaaa
      121 atctaataaa agatattata gaaaacttaa aaataaaaaa taatagtgac atagagactt
      181 ataaacaatc attaaaagaa ttggatgagt acatagctaa atgggttcat gaaataaaaa
      241 ttccaatatc ttctttaagt ataattacag ataggttaag tagcatagaa gatagtttgg
      301 atattaagaa tcaggttgca aagataaatt ttttagtaaa ctctattttg tatagtagta
      361 gaagcaacac tatgtttgaa gatgtattta taaataaatt taatttagaa aaattgataa
      421 aaatgtctat aaaaaataat tcatttttgc ttataaaaaa taatgtagaa gttagtttga
      481 atgaattaga aaatgatgtt tacacagatt ctaaatgcat gtcatatgtt ttagaccaaa
      541 ttattaataa tgctataaag tattcaaagg aagttggtaa gattgaattt aattcaaaaa
      601 agttagaaaa tggagtggta ttatctataa aagattttgg tataggtata aatgaggaag
      661 atatatctag ggtttttgac aagggattta cggggaaaaa tggaagaaat caattgtata
      721 agtctactgg aatggggatg tattttgtaa agaaaatgat agatagttta gggcatgaga
      781 ttgaggtatg ttcggaaaat ggttcatata caatttttaa tatctatttt tatgatatat
      841 cggattattt aagcctagat agttaggtta ttagactata aaatctatca tcatatacaa
      901 atcgacatat acaataagaa taaatttagt tcttgaaaat agtgaagatt taattagtgc
      961 cttaaatgat gctctatcta tattataaaa taataattat tatatatgaa aaacatatca
     1021 gataggtacc tggtatgttt tttatttgta gtaactattt taatatggtt tttggagaga
     1081 gaatatgttt tgtttgtgaa aaaaaattac atggtattac attgacactg aaaataaatt
     1141 aatgtaagat ttagctaagg taaaaaacgt gtataagatt aactcaatta atttcataag
     1201 agtaaattat cgtaattaaa gatgaaataa gattaattct aaaattatta aacttaatac
     1261 cttgatttgg ggaggaataa ctataaactt aaaaggatga taactatgta tagtgtaggt
     1321 ttaatgtcag gaacttcctt agatggagtt gatgcagtac tagctgaaat tagtgggaat
     1381 ggtagaaata ctaaagttaa gcaaatagaa tttataactc tagaaattcc taaagatata
     1441 aaagacgaaa taagaaagtg ctgtatagaa gaggaatcga gtgttgattt aatctgtagc
     1501 ttaaatttta agcttggata tttatttagt aaagctgtaa aatcagtgtg tcataaagca
     1561 aactttcata tagcaaattt ggattttatc gcatctcatg gtcaaacaat ctttcatata
     1621 ccaagaagtt ataataattt tgtatcttca acgcttcaaa taggtgaacc agcagttata
     1681 gcatatgaaa caaatacaaa ggtaatatct aattttagag taatggatat agcagcaggt
     1741 ggagaaggtg ctccattagt accatatagt gaatttttat tatatagtga taacaataaa
     1801 aatttagcac ttcaaaatat tggtggaata ggaaatatta ctataatacc taaatcatgt
     1861 aacatagatg atgtatttgc atttgataca ggaccaggaa atatgataat agatggagta
     1921 tgtcaaagat tatttaatag aaagtatgat aaaaatggat actttgcatc aaaaggtaaa
     1981 attaatgaag aaatgttaaa agatttgatg agtcataagt atcttagtca agcgccacct
     2041 aaaagcactg gaagagaggt atttggacaa gtttatttgg ataacatgct taacaaatat
     2101 aaacatgtag ataaatatga tttgatagca acagtaacta tgtttacggc aaaagctata
     2161 tattataatt ataaaaactt catacttcca aaggttaatg tagatacttt attaattgga
     2221 ggtggaggag ctcataatct aactttaata ggatatataa aagagttatt attagaggtt
     2281 gaagtattaa ctcaagatga atatgggtat tcttctgatg caaaggaggc tttggcattt
     2341 gtaatcttgg gcaatgaaac attaaataat tctttttcta atgtaatatc tgcaacaggg
     2401 gctaaaaata aagttatttt agggaatata acacctaaac cttttggagg taaatgacat
     2461 gtctaataat aaaaaaaaga aaagaaagaa aaataataat tataaaaatg caaaaagtac
     2521 aaaaagtaat aaaaatacta agaaacaaat ggagaaaaat caaagaacag attataatat
     2581 tccaactata agttttaaaa gtggattaat attaatagtt tcatctctag taagtactgt
     2641 agtttttcct ttgttattat ctatatttgg aatagataat agatttggag ttgttatagg
     2701 aaattcatta attacatctt ttgcagttgc atatacaaga tactttgttg aaactaagag
     2761 aggtttttct ttgggatttt ttagactgta tattttattt gctttatcat ttgcaataat
     2821 aggttacttc tggatatata aaggaataca tatttaaaaa agtattgaga ctattttatg
     2881 ttaataataa gatagtcttt tttctttttt aagaaaagat atgagtattt taagtagtgt
     2941 gtaattttag acaaaagaat gattagataa ataaagcaat taaagcaaga tataaatgta
     3001 tgaaataaaa ttacaattta aaatgtaaaa tatgaaaaaa atatatacaa acgtattcct
     3061 gtaatcaaaa atacatgcta taatgattga aaagaaataa aaaaacaggg ggaatcaata
     3121 tgagagtatt atttgtttgt tcatcaggga tgtcaagtgc tatagcagtt aatgcattac
     3181 aaaaagaagg agcaaaaaat ggaatagata tagatgtttt ggctgttggt actcaagagt
     3241 ttgaggatga agttaaaaat ggatgggata ttgctatggt agctccacaa gttagacata
     3301 gatttgacta tttaaaagct tttgctgatg aggttagtgt accatgtgca ttaatacaag
     3361 cacaagctta tagtccatta ggaggaccaa agttattaaa acaagttcaa gagctgttga
     3421 gtaaatagta gttgcagtgt tgttagatta gaagaaagtt aattttataa aaattttaat
     3481 gataataaaa ataaatatta attaaggaga gaaaagatgc tagataaatt tacagaattt
     3541 ttagacacta aattatcaac ttctatggcc agattggcag aacaacgtca cttaagagct
     3601 gtaagagatg gaatcgtagc ttcattacca cttataatag ttgggtcatt tttcttgata
     3661 ttagcatttc ctccattacc agattcatgg ggggtaactc agtatctaaa ggaaaatgca
     3721 gttacaatat tactaccata tagaatgaca ttttttatta tatcagtata ttctacattt
     3781 ggcataggtt atagtttggc aaagtcatat aagttagatg gattgacagg aggtttatta
     3841 gctacagcta cattcctttt gacaatagtt cctactgcaa tagagggtct tacccctgat
     3901 ttattaaaga tagttgaaaa gagtccagaa cttttagctt ggtataattc aattcctcaa
     3961 ggatggcaaa tgccaatggc taatcttggt ggaggttcaa tgtttgttgc tattgtaaca
     4021 tctataatag gtgttgagat ttatagattt actgacaaat ctaaattcaa gataaccatg
     4081 ccagaagcag ttccagcatc agtcacaagg tcttttgaag ctctgacacc agccgcatta
     4141 ataataattt taatggctat tgttacttat ttcctagggt ttgattggca tggcttcata
     4201 gctaaattag taactccatt agtttcagcc actgacacat taccatcagt aattatacta
     4261 atatttttaa taacgttttt ttggtcattt ggaatacatg gtgtatcaat aataggttca
     4321 ttggcaagac ctttatggat acaattatta gatgctaata cagcttcata tgctgcgggt
     4381 caagcagttc caggtattgc tgctgaaccc ttttttcaat ggtttatatg gattggaggt
     4441 tctggagcta caataggctt agctttactt cttgcattta gagcaaaatc agcttatgga
     4501 aagtctttag gaaaagcaac tgtaatacca gcaatattca atataaatga accaataata
     4561 tttggagcgc caatagtatt aaacccaata ttaataatac catttgttgt agcaccaata
     4621 gtaatggcaa cagtttcatg gtttgctatg agtgctaatt tagtatccag agtagtagtt
     4681 actgctcctt ggacattacc aggaccagta ggggcatatt tagccactgg tggagattgg
     4741 agagcttcag ttcttaactt attatgtata gctatatcaa taataattta ctatccattt
     4801 gtcaagatgt acgataagag gttgcttcat gaagaaaaag ttgaagagca agaagcttat
     4861 taattagaaa ctatattgga aattaaaaac tatattagat tagtataaat ttcaaagtat
     4921 aacagttatg aaataagtaa cgaagatatt ttgttgattg tttataaaaa aagaaaaggt
     4981 aaacttttct ttttttataa atatatatga aacttttttt catcaaattt ttaaaaaagt
     5041 ttcgtgtaat tcgaaataaa tttaaacaca tataggatat aaggagatga cacagatggg
     5101 aagattagga atatcaattt atccagaaaa aacaacatct aaggaaatat atgattatat
     5161 agataaagct gctgaaaatg gatttacacg tatattttca tgtttattat cagttaaaga
     5221 tactaaagaa aatataatta aaaaatttaa aagtataaat gaatacgcta agtttaaagg
     5281 ttttgaggta atacttgatg taaatcccag agtatttggt gaattagaaa taagctacaa
     5341 agatttgaat ttttttaaaa agacgaaggc agacggaata aggcttgata tgggatttac
     5401 aggttctgaa gaggcattaa tgacttttaa tcctcaaaat cttaaaatag aaatcaatat
     5461 gagtaataat actaattata tagatacaat aatggactac caaccaaata aagaaaattt
     5521 gataggatgt cataactttt atccacatag atatagtgga ttaaatttag aacactttat
     5581 aaaatgtaca gaaagattta ataagtatgg attaagaaca gcagtttttg taacaagtca
     5641 aaatgaaact acatttggac catggcctgt aactgatggg cttccaacaa ttgagatgca
     5701 cagaaatcta cctatagata ttcaagtaaa gcatataata gctatgaata atataaatga
     5761 tataataata tcaaattgtt atccaacata tgaggagcta aaggtattag gttctatgag
     5821 aaaagatatg gttacatttg atgtagcaat agaggaaaat atacgagaag ttgagaagaa
     5881 gatactcttt gaagaaatac attttaatag aggggacata tctgatagtc tgataaggtc
     5941 tactcaatct cgtgttaaat ataaaggata taaatttgaa ctatttaata ctcctaaaat
     6001 aataaaaaga ggagatatag taattgaaag tagtgaatat ggtcattatg caggggagtt
     6061 acaaatagcc ttgactgata tgaaaaacag tggtaaatcg aatgtagtag gatatataag
     6121 aaaagaagaa ttatttatat tagattatat taaaccatgg caaaagttta attttaaacc
     6181 ctcaacaaaa taatttatta tgaaaatatt aatttattat aaaaatataa ttatgaaagt
     6241 tggaattaaa atgtgtttaa aaatttaata ttaagaaaat gaggaaatga tatgttaata
     6301 aaaactttag aaaatttagt tacagaaggt agaaataaga atacattgca aatagataaa
     6361 gaagatacat taggcataat agaattaata aataacgaag ataaaactgt agcctatgct
     6421 gttgaagaac aaaaggagag tatagcaaaa gctgttaata taatagtaga tagaatgaaa
     6481 cagggaggta gattattcta tataggagca ggtaccagtg ggaggatagg aatactagat
     6541 gccacagaat gtcctccaac ttacggagtg gattttgagc ttgttcaagc tataatagct
     6601 ggtggaaatc aagcaatatt taaagcaata gaaggtgctg aagatgataa agaacttgga
     6661 aagcaagata taatagatag aggagttaca tctaaggatg ttatatgtgg tatagctgca
     6721 tcaggaagga ctccttatgt tataggtgca atggagtatg ctaaggaatt ggggtgtgca
     6781 gtactatcaa taactatgaa tccgaatagt gaaatgagta aaaaagcgga tttaccaata
     6841 aacattatag taggggcaga agtaataatg ggttcaacta gaatgaaaag tggtacagct
     6901 caaaaaatgg tatgtaatat gttaacaaca gcttctatgg ttaagatggg aaaagtatac
     6961 tcaaacttga tggtagatgt taagacatca aatgaaaagt tggttgaaag agcaaaaaga
     7021 ataataatga tagctacaaa tgtaaaatat gatgttgcag aaaaattctt agaagaagca
     7081 gataatagcg ttaaattggc tatatttatg ataaaaagtg gtttagataa ggatagtgca
     7141 aagagtatcc tagatagaca agaaggttat ataagtgaag ctttaaaatc aatagaaaaa
     7201 ttgtaaaata aataatagta ctgataacct ttcaatatta aaaaaacagc attggaaggt
     7261 tatttttaga tattaagtat tgaaagaatt attaaagata ttgtaggagg atagtatgta
     7321 ttttttaggt gtagatggtg gaggaacgaa aacaacattt acattagtag atgaagaatt
     7381 aaatatagta ggaaccataa ccaaaggaac ttgtcattat aatcaaatag gatttgataa
     7441 tctaacgaaa ttattaataa ctgggttgga agaagtttgt aaagatgcta agataaatgt
     7501 agaagaaata acgtatgcat ttgttggact tgctggatat ggaaaaataa aagaagtatt
     7561 gtatgcatta gaagtagcaa caaaaaatgc atatagtcat ataaattata ctctaggtaa
     7621 tgatgtagaa atagcattag caggttcttt aaatggggaa aaaggtataa atataattgc
     7681 aggtactggt tcaatagcac aggctttaga taaagatggg aatcttcaca gatgtggagg
     7741 atggggctat gtattaggag atgaaggttc agcgtattat ataggaatgg ctaccttaaa
     7801 aatgttcaca atgcagtctg atggaaggtg ttcaaaaaca aaattatatg atttaataaa
     7861 aagacatctt aatatagaga atgattatga cataataaaa tatgtaaatg atgaaataaa
     7921 aggagataga atagaaatag caaagtttgc tacgatatgt ttaaaagcag ttattgaagg
     7981 tgataatact gctagtaaaa tatttgatga tgcagcttat gaattaagta ggcttataat
     8041 aggattagag catcattttg aacaaggtac aaagataaag gtatcttatt caggtggagt
     8101 gtttaagtca ggagatttaa tattagagcc acttaaaaaa tatttgaata aaacgagatt
     8161 tggtatagtg agaccagtgt tgactccaga tttaggagca tgtttacttg ctaagaagaa
     8221 atattatagt aaataggtag aggtgataga gtatgagttg cattttaaaa ttaaaacaga
     8281 tatatgaaga tttaacggaa gtagataaaa aaatagctga ttacatactt aataatacag
     8341 aggctataag taagctttct gtatcagaat tggcgtcaaa ctcaaaaaca agtacagcaa
     8401 gtatagttag gttttctaga aaaatgggat attcgggttt tggggattta aaaattgaga
     8461 tagctaaaga tttgatgggc aaagaaaatg aatacacata tgtagatgaa aactatgatt
     8521 gtaatataga tagtgttata aacaaaatta caaacaaaaa catagagact atcaaccaaa
     8581 caaggttgtt gaatgaaaaa gatataataa aagaagcagt tgaacagata ataaaggcta
     8641 aaaatgtata tatatttgga gtaggtggtt cggctttagt tgcactagat ttacagatga
     8701 aacttttaag gataaataaa caggcattta cctctttaga tagtcataca caacttatgg
     8761 tatcttcaaa tgtagataaa gaagatatag ctatagcaat atcttactct ggagaatcaa
     8821 aagaagtaat aaagtctata gaaaatgcaa aattaaaagg atgtaaggtc atttgtataa
     8881 ctaaatatag tgacaatcat cttagtaaga ttagtgattt aaaactagta gtaccaaata
     8941 tagaaaaaag gcttagagaa ggagctattt cttcaagaat agcaatgctt acgttgatag
     9001 atataatata tatttctata attcaagaaa acttaaatga ggcagaacag aaactacaag
     9061 aaacaaaaag gatacttgat tatttaaata ttaataatgc ttaaaactag gttatatatc
     9121 aactaaacaa ataagttaga ctataagcgt aagtcaataa gaaatagata aatatctaaa
     9181 atattgactt acttaataat aatatatagc atttttataa tacaaatgag ttgtcttaaa
     9241 attgattttt tttaatcaaa ttttaagttc aactcctttt ttatattttt atagatatat
     9301 cctatatttt cccagaaaac gtattgaata tacaaattat aagaagtata atgttgttgg
     9361 gagagaatgt atgaaatgta tacaaagttg attttaactg tattattgat aattctcttt
     9421 actcttacca ttattaatga ttgaagacaa tggtatttat tataggtcaa agattccgaa
     9481 tgtattaatc atcatcattt tctcaaacta acagcaaaca ccctgatttg cgagttgtat
     9541 tgtatacaaa ttatctagaa aatatctatt agattttcca tgaaatttaa atatttcacg
     9601 aatgtatgaa tggagaaata tacatgttga caatccacct ttttaaatcc taaattaatt
     9661 ttattaatgg tagagaatag gaagtaacaa tgttttataa aggagaatat taagtatgaa
     9721 gaaaattagg aagttagcta ccaagttatt gattacaaca atagttttaa tctcaggtat
     9781 gagtatgaca gtttatggta tgacagccaa agaggtaaca gcaaaaacac caaaaagcta
     9841 tgtcactgga accaatagcg tatatggtcc aaagctgtca caagcccaac taaactcagt
     9901 agcacaagca acagcagatt ttatgaacaa gaaaatcaca aagaatatga ccacagatgc
     9961 taagatttta gttgcctaca atcacattaa aaataacaca acatatgttg actggaatgc
    10021 agtagaggga gcaaatactg cctatacatt ggttactaaa aaaggtgcat gctcaggaat
    10081 ggctaggagc atgaaagctt tgtgtgatgc tatgggaatt gaatcttatt atgtacacag
    10141 cacatcaaat gaccaccagt ggaatcttat ccgttttggt gatgggcgtt tgtatcatgt
    10201 agatattgat gctaataaat cagcaggaaa ggatatcata tacaagagtc ttagccaccc
    10261 attaccattt gataagacag catatccaaa agttggtgca atagtacaga cggtaaaacc
    10321 agaacagaca cagaaaccag caccacctgg gaagcacact atgttaccac atcaagaagg
    10381 ggcaaaggct gttggaaatt ttgggaaaaa aggagaagat ataaatgaaa aacatgtaat
    10441 gtatgaaggt gttcgatgta gtgtagttaa ttcatcagta aatacaatta catttacagg
    10501 tagtgaatca aatttagacc catcaaatct tacgattgct gtaataggat ttatgaacac
    10561 accatatggg atgtttgcat tagcaccaga gggtaaacct gttgaatatc cttttaaata
    10621 tgataagcca ttttctttag attttagtgg taaaaatcgt tctggtgcag aattagcgaa
    10681 gtactgtaaa aaaaatgatg tgactattaa aatagaaatc actgatgaaa aagctaatat
    10741 tggacttggt caaataatgt atgttcgata tgaataatga tttaatcatg acagtagaat
    10801 aagttataaa cattgcgccc tcttactagg ggcgcattta atattatatc tgatattttg
    10861 tagtcaggtc aactgctaga tttctcccct ttttatttat aaaaattatt atctctaagc
    10921 atctgagcat gtttacgatt aactaaaaac tgttttaact cagtcaagca taggaataaa
    10981 atagctctat tttcaaactc tgttctgtca acaggtagag gctggttttt catactaagc
    11041 attagtctct ttaattcttc aagaagaaga ttgcctgttt cagcttcaaa ttcagtataa
    11101 cctatcttat gtataaaagc agatagaata tgagcttgtg gtggtgtaga atttagttga
    11161 gatacataac tatacaaatt ttctaagata tcccgttgac tttttataat atccatatgc
    11221 tctaagaaga atctagtatc acttaataaa gtattattaa tattatcgta agtttcagaa
    11281 atactgctgt caataagtga atttaatgta tataaatctc tactatatcc attttctttt
    11341 ttaggattta caataatatc tgcaatgtct ataagtacaa tactcacttc ttcctgtaac
    11401 tttttttgaa cttcatggat tttatgtata tttgttggaa tatacatatt tattaagaca
    11461 cctattccag ttcctactgc aaatattcca gcttcattta aaatccattt gatagaagtt
    11521 tctccttgaa ggtagtaatg ggatgctatg actacacaca tagcaataac atcttgaata
    11581 tttaacaaaa aacaaagtgg tataattata aatataaata ttgaaaatga agtcaaatta
    11641 tatcctaaaa tattaaaaca taaatatgaa aaaatagtac ataaaagaaa accataaatc
    11701 tttccaatgg agcctttaag agtttctctt ttagtgtcct ttactgtaag tagtactata
    11761 actccagcaa ccattgaata ttctaatttt aatagccaag caataaatat agcaagagaa
    11821 catccaagag ataattttat tactttaagt gaattaatat tattcaaatg caaacctcct
    11881 gtaaatcaat ataataaaat tctaacataa aactaatatt gcttgaaagg taagtaacta
    11941 tacttaatta ttttattgcc ctatttgttt agatacaatc aatataattt tattttatga
    12001 aaattttttg taaaatttta aatttagaga ctaacatgag accacaaaga ggaagtttat
    12061 tataagaaga aaacttaaaa ttgattttaa atagcaaatg aaaataatca aatatgtata
    12121 tactattatt aaaaaataaa attatatagt taatataatt ttataattgg tttagtatag
    12181 agatttttaa cttttatgtt atactattta taaaagtagg tataaagatt aaaatacata
    12241 atcaaatatg tataagaggt tttaaatagt attgttaatg aacttgatta ttgtttataa
    12301 ttacaaattg gaggagataa acatgaaaat tactgaagga tatatgcctt ttaaaggttt
    12361 taaaacgtat taccgtatag taggagaaaa tacggaaggg aagaagccat tagtattact
    12421 tcatggaggt ccaggttcta cacacaacta ttttgaggta ttggataaga tagctgaaag
    12481 tggtagacaa gtaataatgt atgaccaaat tggttgtgga aattcatttg tagaagggca
    12541 tcctgagcta tttaatgcag atacttggat agaggagctt attgaactaa gaaaacatct
    12601 aggacttgat gaaattcatc tgttaggtca atcttgggga ggtatgcagg ctatttggta
    12661 tgctattgaa tataaaccaa aaggtattaa gtcttacatc ctttcatcta ctctttcttc
    12721 tgcaaaactt tgggagaaag aacaaaaaag aagaatatca tatatgagtg aggttgacca
    12781 aaaagcatta cttgatgcag taaatactgg agattactct agcaaagaat ataatgatgc
    12841 attagaaaga tttatggaaa tgtattgtgc aggtgaagtg acagaagatt ctccagagtg
    12901 cctgagaaga cctaaaaaat caggttctga agcatatatt gtaggatggg gacaaaatga
    12961 attttctcct acaggaactc tttctggata tgaatttaca gatagattac atgaaataaa
    13021 agagccttgt ctagtaacta gtggagcaat agatttatgt tcaccatata ttgcaaagac
    13081 tatgtatgat agaataccta atagtaagtg ggaattattt gagtattcaa gacatatgcc
    13141 attcgttgaa gaaaatgaga aatatataaa agtcttaact gagtggttga atgcgaacga
    13201 ttaaaaacta tataaattta tataaaaaaa tgagtgtctc aaaatgaact ttttagttca
    13261 ttaggcactc tttttgcatg aaatcaaata tattttcatt atttgaacga taaaatgagg
    13321 cttatatcta ttttgggaaa gtaatttttt attactagcg ttagattcta atctaattgt
    13381 atacttattg aaataataga aatatattgg tgtttataac ttaattttat tgttttttag
    13441 aaattttttt atatattggt ataattattc tgtttaaatt tgattaaaaa gtatattaat
    13501 tataataaga aacaattata taaataggaa tagattatta taattaatat agatataaaa
    13561 ataattatag aaggaaaggc acattatgga aaaattgaga gttattgaaa tgataaaaaa
    13621 taatcccaat attatagcta caatagataa tccaactgat gaaatgaaat tgttagctat
    13681 taaagaaaat gggcttgtac tagaatatat aaataatcca accagagaga tgcaagaact
    13741 agcaatagat aataatatta gagcaattaa atttatagat aatccaacag aagatatgat
    13801 gataaaagca gtaaatgagg gatggagtat actggattat atcaaaaacc caactgataa
    13861 agttattgaa atggctataa atcaagcagg atgggcaatt aaatatgcta aaaatccaag
    13921 tgaagaatta caactgttag cagtaagaaa aaattatgat tcgataaagt ttataaaaca
    13981 accttatgaa agtgtacaag aggaggcagt gagaataagt tatgatgcct tgagatatat
    14041 agattcacct agttataatg cagaacttat agctataaaa aataatgaag cagctattag
    14101 ctttataact gatttagata aaaataaaat gttggaattt ttaaaagtga atattttagt
    14161 gattaagtac attctgaaaa aagtatctaa agatgaatta gagagtgcat taaaagaagt
    14221 attatccaaa gaagatgttg aggaaaagta tattagagat tttttgaatt gtagtgttat
    14281 agatagaaat agcgaaagta taccaataga taaaattatg tttatttata aatatggaag
    14341 taagacagca aaaaaaatag ctgtagatga gaaattaaag attatgtagg agagagaaaa
    14401 atgaatttta tagacacatt aaaaagcgtt gacttaatat tatccactgg agaagtacct
    14461 ttaatagtag gtgaaagtgg aataggaaaa acagctttag caaataagct tgccaaagaa
    14521 aatgattgga gtttaattgt tattgatgga aacttactta aagagggtga aataggtggt
    14581 cttccaacta tagaatctta tgtaggagtt aattctaatg gatataagac tgaaaagaaa
    14641 acaacagtgt atgctgttca taacaaatta agagaaattg atgaagagat atctaaaagt
    14701 aagacagttc ttttatttat agatgagata aatcgttgtg agcatacagt acagcaagaa
    14761 cttatgaatt taattttaaa tagagaaatt aatggatata aactgcatga tgatgtaaag
    14821 attttagcag caatgaatcc atcaagtaaa tatggttcag attttgatta tcaagtggta
    14881 gatatggatg cagcccaaga aaataggttt gtttggttaa atatggagag tgaccataca
    14941 caatggataa aatgggcaat agatgagggt attgaaagaa aagttatcga gtttatttcc
    15001 acttttccag aatatctgca taaaataaat gaagatgatg taagagccac tcctagaagt
    15061 tatgagagag tatctaaaat ttataaagtt tataaagaaa aaaataattc tattcctaga
    15121 gcagtatttt taaatgttgt aaagggtaat gtaggtaaag ttattgcaga agaatttata
    15181 agttttattg aatcaaactc tgaaccatta atatcctatg aagatgtttt tttaggagaa
    15241 tctattgatg agtctattgt agaaagagta aaaaatgaga gtcatacaag actttatcta
    15301 tcagctatga acattctaaa agacttagaa ttaaatatta agaatgataa atatgaatca
    15361 aaccactata ttaatagatt tattgaattt ttaaaaatgt atcctgtaga tttaatgata
    15421 ggtattatga aggatattag aaacagttat attgaagtat ataaaaaagc tatagaaaat
    15481 gaagagtttg taaaatcata ttttgaatct tatagtttaa taaggggata atgtatggaa
    15541 aattattttg ataaacaagc aaaagagctt tataataaag caagtgaaat tataaatact
    15601 cattctatgc taaaggcaaa tcgtgctaat gagaaatttg atatagatat accacaagat
    15661 tttaagtatg agttttttag tcttgtagat aaagtgaatt taagtcttat ggaagaagaa
    15721 gataattttt atggatattt tttgtttcag atgtcaagag aaataagatt tgatataagt
    15781 agtcctacag gtgtgaattt taagggagct aaatatatta tatattttaa tccaataatc
    15841 tttttaacgc ttgacataaa gcaaatggag actacaatta aacatgaaat tcatcatata
    15901 ttatctatgc atttgatgag agcaaaggag ttaaaaggaa agtatagtac attagcaatt
    15961 aatatggcaa tggatatagt agtaaataaa tttctaaaca atttaccacc atatgcaact
    16021 accttagagt gggtaaattt aaaatattcc ttaaaacttg aaccatatga accttttgaa
    16081 tattatgtag aaaagattca gactgaatta gatttgatgg aagtggatga agatggtgag
    16141 gaagatgata gtgataaata tggagatata gaaattgatt atagccccga aagaactcat
    16201 gatatatggg atgaatccaa tgagatagat gaaaataccc ttagagaatt tactgaaaaa
    16261 tttattaaca gttctcaaaa aggtaaagtt cctgcttatt tagaaagtat gatatcatca
    16321 cttaaaaata gtaagggtga attgccatgg aatttatatc ttaatagaat aatgggaaca
    16381 gttgaaaata ataaaaagaa gactataaca agaagaaata gaagacaacc aaatcgttta
    16441 gatttgaggg gagaacttag aagtcataag tcagaaatag cagttgcact tgatgtaagt
    16501 ggaagtatta gtgatgaaga gtttaaacaa gctattaaag aagttcttag tatagtgaaa
    16561 aactataatc atgaaattac aattatagaa tgtgataatg aaattagacg tgtatataaa
    16621 gtgagaactg aaaaagatat aaaagataga attaggatag gtggaggtac tagattttct
    16681 ccagtctttg agtatgctaa taataaaaag attaatttgt tagtctactt tactgatggt
    16741 aaaggtgaaa ataaactaca agtaatacct agagggtaca aaattttatg ggttatttct
    16801 ggaagaggag ataaaatttc attaaaagaa ccttatggag cagttaaaaa acttactaag
    16861 gttaaaacaa atgatgaaat attagaaata aatgatgtaa gaaatgatgg gtactcaatg
    16921 aatagccaag caccaatttt ataggtattc acaggagata agctgtatta atttattttg
    16981 atatgcttgt tataaaaatc taaaaaatac attaatcaat ggttaatgta ttttttaatt
    17041 ttaataatat aagttttact tacatttaag tctcaaaatg tgttaaaata taatatatag
    17101 cagatagttt tggcaaggtg gatttagttg tcaaaactat tttttttatt aaggatggtg
    17161 atgtttttgt ttaagattga tgattatata atgtatggta tgacaggagt ctgcaaagtc
    17221 atggatataa caaatgaacg atttacaaat ggaataaaaa aagagtatta tgtattgagt
    17281 cctatttatt ctaataatac agtaataaaa attcctgtag ataatgaaaa agtaccaatg
    17341 agaaagttac tttctaaaat taatgtgctt tcattaataa atgatattcc taatatggat
    17401 acatcatgga tagataatga aaaattgaga agtgagcaat ttaaaaaaat actcagaggt
    17461 ggtaaatgtg aagagttaat aaaattagta agaagtatag ataataacag agagtatgta
    17521 aaatctattg gtaaaaaaac acatcaagca gatgataata taatgaaaga agctgaaagg
    17581 ttattaaatg aagaatttgc aactatttta gatatttctc ctaatgaagt tagttcatat
    17641 atttcaagtc atatacctca ataaagtgtt ttagtatcct ttatttaatc tattttaagt
    17701 attatttatg tatgctggtt ttggtaaaat aaaatatata attaaattat ataaaaaata
    17761 ataatgattg tattgaagaa ttacagaata gtgcggagag cctactcctt taggtgtgag
    17821 atggatatca cttaaagatt taaattgtta ttgtagatgt ttttaatata aaacaagatt
    17881 aataagtgta ttgtaagctt taagtgaaat tgatactata atgagttact tagattttaa
    17941 aatatataat attataaaaa atccttttag gttttgaatg aataagatgg atactatgtt
    18001 gaatagaatc catcttattt aagttttatt tatgttgttt atttgtaagc attatttttg
    18061 tacatgaaaa cacagtgtaa aaatatgctt agaattatga agttttatta aaaagatatt
    18121 cgacaaaata agactaaaaa tattcttaaa cttataacta agataaatat tgtatattta
    18181 tagtgtagcc tgaaaaattg tggatgtacg aaatttattt agtattttat tgaaacaaag
    18241 tagaagaagt actatagttt atctttgaat agattgttat tttttagtga aaaagttatt
    18301 aggttaatac ataaatataa tagcttataa atagtttaag catgttaaaa aagtagttat
    18361 tagagatata tagttacatt gcttataaga tacttaaaat gatagcattc acttatattc
    18421 agaagattaa gataaaaaat cattttta
//
LOCUS       DAMJYR010000068        18439 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.068, whole genome shotgun sequence.
ACCESSION   DAMJYR010000068 DAMJYR010000000
VERSION     DAMJYR010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 18439)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18439)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18439
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.068"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(231..911)
                     /locus_tag="PC471_002324"
     CDS             complement(231..911)
                     /locus_tag="PC471_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2789451.1"
                     /translation="MQTKILIIDGDKDNCKKLKGFLEEKGISIDLAYNCEEAIGKIFS
                     NKYDLIFLEIILSDGDGWTLCKKIRNVTTCPIVYMTYINEDQSILNALNSGGDDYLIK
                     PLNLEILYAKVKAILRRMNSYVNNNENNTKLEEYNRTSGVIKLEDKIIKLTPTENKLL
                     NFFIENPEKTLTTKEIYEHVWMNEYLEDNYSVVVAVNGLRKKIEKDYKNPQKIITIRG
                     AGYYFNKE"
     gene            complement(961..2976)
                     /locus_tag="PC471_002325"
     CDS             complement(961..2976)
                     /locus_tag="PC471_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HDF2789452.1"
                     /translation="MKKYNILKGTLIVLIVSLLAITGLNLLYKYDNKYTYKSIRASNG
                     ILTIDNDTFTKGKLVFLIDDWEFYNHKIFKPNDFTEQDIKPEYVFIGQYPDFSMRKGT
                     QSPYGNATYRMRIKNEGKDKLLSLELPEIFSASEVWINGEMVSKLGDVGTEKYRPKIK
                     NSVVSFIAKKNTEIIINVSNFSHYYSGLYYPPALGETKDISNMMFYRLLFYSIICFTT
                     FAIAIFSLSVWFLSNKSKIYLYFGCMCVFFAIHVSYPFVHLLGLPLVSFTYAVEDLSY
                     FMVVLCIICINGSISKIEENKVYRFLILPLGITMSIISIVIPIILLPYDMYFVNIYGK
                     LIDIYKYIVFIYILMSSLVSIYKKTCSEHISSYILVSVNTVFGISILFDAVTSNRFEP
                     IFTGWQTEYCGFVIVILFSVMMINRNRIIIKENEKLTRNLEHEVEVRTNELTTLLNER
                     KRFLADVAHDLKAPVSAIQAFIDLIKVGNIHVDEETRTYLYAINQKSNEVQNRVRSLQ
                     EFTSQDKSIGISEKICLNNFIKEIYENNLPDTQACGVNFETYVLEKKIYAKINKEMLT
                     RAFENLIYNALTFTPFDGKIILSMDKEDGFAIIKISDNGKGIPPENIPKVFDRFFTER
                     DNNEPKGQGLGLYIVKSIIREHGGEIFVDSELGKGTVFTIKLSLYFL"
     gene            complement(3048..3725)
                     /locus_tag="PC471_002326"
     CDS             complement(3048..3725)
                     /locus_tag="PC471_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2789453.1"
                     /translation="MYNLLVVDDDVDILNINQIYFSNIGFNVYTSETAKDAMDIVESI
                     VLDCIILDISLPDIDGYKMCNDIKEKVNIPIIFLSNYEHEEEKVRGFLAGGDDYITKP
                     YSLKELELRIYARMRQYKNITSVPNVLKFSALVINVNSRKVTFDGESIDLTTMEFEIL
                     LFLAENKEQVFSKIEIYNQVWKMPDIGDQHTVQVHIAQMRKKINSLSREHQYIQTVWG
                     KGYKFVP"
     regulatory      complement(4305..4388)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01786"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01786"
     gene            complement(4621..5463)
                     /locus_tag="PC471_002327"
     CDS             complement(4621..5463)
                     /locus_tag="PC471_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein"
                     /protein_id="HDF2789454.1"
                     /translation="MFKKKIGVLLASTMLVGALAVGCSSNSGGSGDSGDSSKVSVSGS
                     TSIGPLMETIGEKFQEKNQGVSVEVQQIGSSGGIKNAIEGTSEIGMSSRDLKDEEKSA
                     GLKETKIAIDGIALITNTANSVKNLTMDQVKGIYTGKITNWKEVGGNDAPIVIVSREE
                     GSGTRDGFQEIVGFDSEELTKDAQISDGSGNIKTTVEGNENAIGYISFGYVDEKVNSL
                     KINDVEATDANVIANKYPISRPFILVNKEDGLSEKGKALIDYILSDEGQALVSEKGFI
                     KVNQ"
     gene            complement(5715..7499)
                     /gene="pepF"
                     /locus_tag="PC471_002328"
     CDS             complement(5715..7499)
                     /gene="pepF"
                     /locus_tag="PC471_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HDF2789455.1"
                     /translation="MGTDRSTIEEKYKWKIDKMYSSKEEIEKDISKVKNLIQEVKEYK
                     GKLSESKENLYKVLNISENASRIVQNLYVYTHMKQHEDTRINDNQGRATKTEMLSTDL
                     GVATSYIVPEILAIEENKLSEYLKDEKLSFYAKYIKDILRDKPHTLSEREEEILAATS
                     ELSTIPENVYDMLAYADMEFPEIEDEEGNKVKLSHSNYSLFIKSKNSRVRKDAFEGEF
                     STYEKYKNTFASTLYGGIKSEIFYAKTRKYNSAIEASLFSDDVSLDVYNNLISAIDKS
                     IPNLNKYVELKKKFLGLNEIHMYDLYVPLTDKFDMDIPYDKAQDIILEALKPLGEEYL
                     KVIKSAFDEGWIDVYDNEGKKGGAYSWGSYDSHPYILMSYKNDLNSLFTLIHELGHSV
                     HSHYSRTSQPFLYSDYKIFVAEVASTLNELLLINYLLENSKSKDETIYLLNYYLEQFR
                     TTVHRQTMFAEFEKIVHQRVESGEPLTADEFTNIYYKLNEKYYGKSAIVDKQIGIEWA
                     RIPHFYSNFYVYKYATGFSAASALSQQILSEGSTAVDRYINFLKSGGSEYPLEQLKSA
                     GVDMTKKESIEEALNVFAELVNKLEKEL"
     gene            complement(7565..8230)
                     /locus_tag="PC471_002329"
     CDS             complement(7565..8230)
                     /locus_tag="PC471_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HDF2789456.1"
                     /translation="MIDIGSKEIAIRLLLALIIGGIIGLEREKIRQFAGFRTHILIAI
                     GSCIVSITSIQLFFDYNLYTNADPARMPAQVLSGIGFLGAGAILKNKGGVKGLTTAAG
                     MWATACIGIAVGYGYYELAILGGLFVMIALFVLRIINTFLFSQEKNVLILKLSNLDSI
                     PFLYDKFEKNQLIVRNMEIECRNEEYWKVNFFVSYAKRIHLHDVMKEINMIEGVINVD
                     YLD"
     gene            complement(8381..8542)
                     /locus_tag="PC471_002330"
     CDS             complement(8381..8542)
                     /locus_tag="PC471_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartyl-phosphate phosphatase Spo0E family
                     protein"
                     /protein_id="HDF2789457.1"
                     /translation="MANNELEALKNEIEAVREEINTYIEYPEIFQEELVESSKKIDIL
                     INKYMYLSK"
     gene            complement(8620..9129)
                     /locus_tag="PC471_002331"
     CDS             complement(8620..9129)
                     /locus_tag="PC471_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HDF2789458.1"
                     /translation="MKTRKMTFLGLMIGYSLALYVLETYIPNPLIVIFPGAKLGLSNI
                     ITLISLMLLGVKDTVIIVTIRVILSSIFAGPLSYLLFSIGGAYLSLLFMYLASKIKDL
                     SLIGISIIGAIGHNIGQLIVASLIIENILAIGYLPFMLMASLVTGMFVGLVSRYTIPK
                     LKHFYSKFY"
     gene            9335..9553
                     /locus_tag="PC471_002332"
     CDS             9335..9553
                     /locus_tag="PC471_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HDF2789459.1"
                     /translation="MTVYDLKIGEKGFINSIDGDIKLSKRLLALGCINSTEIEVKKVA
                     PLGDPIIIRFRGFDLAIRKSDAKNISLQ"
     gene            9571..11517
                     /gene="feoB"
                     /locus_tag="PC471_002333"
     CDS             9571..11517
                     /gene="feoB"
                     /locus_tag="PC471_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HDF2789460.1"
                     /translation="MINVALLGNPNVGKTTVFNLLTGSNQYVGNWPGVTIEKKEGFLG
                     KEIKVVDLPGIYAMDTFSNEEKVSKSYLENEDVDVIVNVVDASNLSRNLYLTTQLMQF
                     NKPIVILLNMLDIAESKGVNIDAKKLSEELGVIVVPIIAKKKNGVDRIEEEIKGATQK
                     AFIYQKNFGSETETYKKLESILSRCTKTTYTQKKSISDKIDNIVLNPILAYPIFIGVL
                     FLLFKFTFDWVGGPLQEGFASLIEAYISAPVSDMLSNSSPWFKSLIVDGIIGGVGGTL
                     PFFPLIFTLFFGISLFEDSGYMSRTAFLMDKVMRKVGLSGKAFIPIVMGMGCSSPAIM
                     ATRTLESEKDRRVTALIAPLMTCGAKLPIYALFVAIFFPHNAALVTTSLYLVGIVMAI
                     VVALFLNKTAFKTEAEPFILELPEYRIPTIDALIKNTWNKSKGFLIRVVTVMFAMSVV
                     IWGLSSFNVFGYTEDINVSFLATLGHIISPIFKPLGFDDWRTSVAILSGLGAKEIVVN
                     SLNILYGNLTVVLPTIFNGVTAYTFLIFTALYTPCIAALATMKKEYGNKMMFTSFAYQ
                     FILAWIMAFIVKSIGGVLFMGNSIIEALIGGIIIVLALFILFNKFKSTKNGNVCSGCS
                     ICSGCPSANSCSSKEDSNKKNNAH"
     gene            complement(11696..12751)
                     /locus_tag="PC471_002334"
     CDS             complement(11696..12751)
                     /locus_tag="PC471_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HDF2789461.1"
                     /translation="MTIQDYMLETPVRMREIISNADSLFNEVKRTNLKKIIITGSGTS
                     YHSGVQVQPYLQNLLDIDVVKMYPFMITEDTFKFDNENTLVVGVSQGGSSYSTYNAMK
                     LAEDKGCKIASMAGCKNALIDEISDYILTVNCGEEKSGAKTKGYYCTKLNLMLLGLQI
                     AREKGIISSEKYNEEINKILDAINRFEAVYKLSKQWIERNKEKLVNSKEIRIIGHSDI
                     YGDTLEAALKLLETMRIPVTGYEFEEFIHGIYNAINSDSTIFILDTGKEPRVTKMIDV
                     LSGWTENVFAIGRDVTENDKNLKIDITDNPYYQTFNFIVPIQLICGEIPTLRGVDPSV
                     PKDTRFHMKLGSKKLNK"
     gene            complement(12799..13599)
                     /locus_tag="PC471_002335"
     CDS             complement(12799..13599)
                     /locus_tag="PC471_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HDF2789462.1"
                     /translation="MNKLTKKELRSMFWRSFALQGAFNYERMQNLGYCYSMLPAIKKL
                     YNKKENQAKAIERHLEIFNTTTVVVPAILGITAAMEEENANNPEFDESSISAVKTALM
                     GPLAGIGDSLFWGTFRIIAAGVGVSLAKEGNIFGPLLFLLLYNIPAFALRIFGLKYGY
                     QVGVNSLERIQREGLMEKIMSMATTVGLFVVGGMVATMLSITTPLKFNLNGAEVILQD
                     ILDKIIPNMLPLTFAFVIYYMLKRKVSVTKLTIGTIVTGIALHAIGLL"
     gene            complement(13592..14383)
                     /locus_tag="PC471_002336"
     CDS             complement(13592..14383)
                     /locus_tag="PC471_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDF2789463.1"
                     /translation="MLVQATMLAIIAGVGILDGRIFGQSMLDRPIVTGMLVGLVLGDI
                     KSGIMIGAQLELIWMGIAGIGAATPPDVVTGGVLGTAFAILSGNGAEVALAVAVPVAV
                     LAQSLGVLVRIINSYFSQKAVFYAKQADFKKVTIMMWLPVILFFLSTSVPTFLAIMIG
                     ADKVTALINAIPKVILDGLGVAGTLLPAVGFALLMDMLLSKKMSVFFFIGFLLASYGG
                     LDITAIALFGVCVAFVLNFYINNKENNQNPQLAINTLTEGEIDFE"
     gene            complement(14423..14845)
                     /locus_tag="PC471_002337"
     CDS             complement(14423..14845)
                     /locus_tag="PC471_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIA"
                     /protein_id="HDF2789464.1"
                     /translation="MKKFLIATHGELSRELVDTSKLIIGSLTNIEYFCMTTDKSADDA
                     EKEIRSILSNNKDSEEFVVLTDVFGGSVANICTNLLLQGFKFELLTGVNLPMLLSILL
                     LVEEDTKLIVKSGLEEAKNGIVYVNELLENQNKIKNVM"
     gene            complement(14861..15340)
                     /locus_tag="PC471_002338"
     CDS             complement(14861..15340)
                     /locus_tag="PC471_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDF2789465.1"
                     /translation="MVSLMRIDDRLIHGQVAYGWATSLGINVILVANDEAKNDQMKAM
                     SLNLAKPSNVTLYIRGVEESGEIVEKFASSKKSNVLVLVKNTKDALVVAKNSGGVLKE
                     INVGGLRYEEGKKKLTDLVAVDDDDINNFKQIGDLGIDIEFRMLPRDKKKQLSDLIK"
     gene            complement(15472..18228)
                     /locus_tag="PC471_002339"
     CDS             complement(15472..18228)
                     /locus_tag="PC471_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDF2789466.1"
                     /translation="MRDEILSFVNECVLFNNFDELKKESGIITEDIVKKFNLSRTQSS
                     RLLNDLVKSDNLIKINSRPVIFLPKNKINDVVGPTKKSIYENLDELKNECKLLKQETV
                     FNSIIGYDNSLKEVIEQIKTAVLYPNGGLTIMLTGESGVGKTFLAKIIYKYSVSSNVL
                     EKNSPYNVLNCAQYYNNLELLSSLLFGHVKGAYTGANESRAGLIEQSDGGVLFLDEVH
                     RLNSEGQEKLFTFMDTGTFSRIGENGVIRKARVRLIFATTESLSEFLQTFLRRIPINI
                     YVPNIEERGTIEKRKIVEHFIYEESKVLGLPIEITQITLNAILKIKFKGNIGECKNVI
                     KYACGRAYAKNQRRNGNIFVTLQDLPRNLYIENVDIFQFQSGKSKSIIFSPKQFSIEM
                     SNGRNKRNIIYQSFIKCLDYFDRLEKNEITKEKFSKKSSNLMIRLMDNLVFSNNDENN
                     NVLMELIISIMQDIFRNLEVNNNIKYDGNNVLAFSSYLYLKDESYQLSDEYKALENKL
                     LIYLKKESYKEYSIISKISTLITNRLDMYLNNVDIIIMIIFIKSTIIRMKFNQVNATI
                     IAHGYATASSMANVCNRMLGKNIFTAIDMPIDATIADVSKQMSRYLEGDRIKNGLIVL
                     FDMGSLNLIYEDLKNKINSPMLFIDQISTISALEVGNLLMQGKNIEEIAEIMDEETKP
                     NIQLYYPQKNKEYAIISSCFTGIGTAIKIQHLLSESLIGIVDVKIIPYDYDSLVSMGD
                     KEPVFEMYDVMAIVGTANPNVNGVDFILLEDIISGKGEDDVFRIFSRVVENEKIKNIN
                     DSIVKNFSLIRVIDSLTILDSKKIIERIEEGINAIENIQKKKLSNDKKISLYVHLSCM
                     IERLVRQTEIEEYTDMDLLIKNHHSEVKLIKNAFSVLEKSYSVQIPISEIGYIYNIIY
                     GLPQ"
BASE COUNT         6036 a         3150 c         2027 g         7226 t
ORIGIN      
        1 aatatataga tttagatata caatttatct atcttttcaa atataatcta taaaataata
       61 gttccattac gcattttttt atataataaa actgactgta atttaactgg caaagtctag
      121 aagaataggc tttatcaatt aaattacagt cagctatttt ataataattt agtttagata
      181 ttgttagtat ttcacattta taacaattat taatgatatt aatatattta ttattcttta
      241 ttaaaataat atcctgctcc tcttattgtt ataattttct gaggattttt ataatctttc
      301 tctattttct ttcttaatcc attcactgca actaccacac tatagttatc ctctaagtat
      361 tcattcatcc atacatgttc atatatctct ttagtagtca aagtcttttc aggattctct
      421 ataaaaaagt ttaacaactt attttctgtt ggagtcaatt ttattatttt atcttctaac
      481 tttattactc cactagttct attatattcc tctaattttg tattattttc attattattt
      541 acatatgagt tcattcttct taaaatagct tttacttttg cgtataatat ttctaaattt
      601 agaggcttta ttaagtaatc gtctccacca gaatttaaag catttaaaat gctttggtct
      661 tcatttatat aagtcatata aacaattgga caagtagtta catttctaat ttttttacaa
      721 agagtccatc catcaccatc acttagaatt atttctaaaa atattaaatc atatttattg
      781 ctaaatattt ttccaatagc ttcttcacaa ttatatgcta aatctatact tattcctttt
      841 tcctccagaa atccttttag cttcttgcaa ttatccttgt ctccatctat tatcaatatc
      901 ttagtttgca ttataatcac tcccaaatat cattattctt ttatattaaa taagctatta
      961 ttataaaaaa tatagactta attttattgt aaaaacagtt ccttttccta gttctgaatc
     1021 tacaaagatt tctccaccat gctctcttat aatggatttg acaatatata gccctaaccc
     1081 ttgccctttt ggctcattat tgtctctttc tgtaaagaat ctgtcaaata cctttggtat
     1141 attttctgga ggtatacctt ttccattatc agaaatttta ataattgcaa atccatcttc
     1201 tttatccatt gataaaatta tctttccatc aaatggagta aaggtcaaag cattataaat
     1261 taaattctca aatgctcttg taagcatctc tttatttatc tttgcatata tttttttctc
     1321 caatacgtaa gtttcaaagt ttacaccaca tgcttgtgta tctggcaaat tattttcata
     1381 tatctcttta ataaaattat tcagacatat tttttcacta ataccaatgc ttttatcttg
     1441 tgatgtaaat tcttgcaaac tacgtactct attttgtact tcatttgact tttgatttat
     1501 agcataaaga taggttcttg tctcttcatc tacatgaata ttaccaacct ttatcaaatc
     1561 tataaatgct tgtatagccg aaacaggtgc ctttaaatca tgtgctacat ctgccaagaa
     1621 tcttttacgt tcatttaaca atgttgtaag ttcattagtt ctcacttcaa cttcatgttc
     1681 aagattcctt gtaagttttt cattctcttt tattattatt ctattcctgt ttatcatcat
     1741 aacagaaaat aaaataacaa ttacaaaccc acaatattct gtttgccacc ctgtaaatat
     1801 aggctcaaat ctattactcg taactgcatc aaataatatg ctaattccaa aaacagtgtt
     1861 tactgaaact aaaatataag aacttatatg ctcagagcat gtctttttat atattgaaac
     1921 cagcgaagac attaatatat atataaatac aatatattta tatatgtcta ttaacttccc
     1981 atatatgttg acaaagtaca tatcatatgg aagaagaata attggtatca ctatagatat
     2041 gatactcatt gtaattccta atggtaaaat taaaaatctg tacactttat tttcttctat
     2101 tttggatata cttccattta tacaaataat acataaaact accataaaat atgacaaatc
     2161 ttcaaccgca tatgtaaagc ttaccaaagg cagtcctaac aaatgtacaa agggatagga
     2221 tacatggatg gcaaagaaaa cacacataca tccaaaatat aagtaaattt tacttttgtt
     2281 tgaaagaaac catacagaca atgaaaatat agctattgca aaagttgtga agcaaattat
     2341 actataaaaa agtaatcgat aaaacatcat attacttatg tcctttgttt ctcctaatgc
     2401 aggcggataa tacaaaccac tataataatg tgaaaaattc gatacattga ttataatttc
     2461 tgtatttttc tttgcaataa aacttactac tgaattttta attttgggtc tatatttttc
     2521 tgttcctaca tctcctaatt tagaaaccat ttctccattt atccatactt cacttgcgga
     2581 aaaaatctca ggaagctcta aacttagtag cttatccttg ccttcatttt ttattctcat
     2641 acgataagta gcattcccat atggtgactg agtccccttt ctcatagaaa aatctggata
     2701 ttgacctata aaaacatatt ctggttttat atcttgctct gtaaaatcat tcggtttaaa
     2761 tattttatga ttataaaact cccagtcatc tatcaaaaag acaagtttac ctttagtgaa
     2821 agtatcatta tctatagtta aaattccatt acttgctcta atagacttat atgtatattt
     2881 attatcatat ttatacagaa gatttaatcc tgttattgcc aacagagaga ctatcaatac
     2941 aatgagagtt ccttttagta tattatactt tttcattgta tcaaccacat ctctttcata
     3001 gtagtattaa ataaaacaag aactatttat taattacagt atctcactta tggtacaaac
     3061 ttatatcctt ttccccatac ggtttgaatg tattgatgct ccctgcttaa agaattaatt
     3121 ttttttctca tctgtgcaat atgaacctgc actgtatgtt gatctccaat atcaggcatt
     3181 ttccaaactt ggttatatat ctctatcttc gaaaatactt gttctttgtt ttcagctaga
     3241 aacaataata tttcaaattc catagtagtt aaatctatac tttctccatc aaaagttact
     3301 tttcttgaat ttacatttat tactaacgct gaaaacttta acacattagg aactgatgta
     3361 atatttttgt actgcctcat acgagcatat attctaagtt caagttcttt taaactataa
     3421 ggttttgtta tataatcatc ccccccagct aaaaatcccc gaactttttc ttcttcatgc
     3481 tcataattag ataaaaatat tataggtatg ttaacctttt cttttatatc attgcacatt
     3541 ttatatccat ctatatctgg caatgaaata tccaaaatta tacagtctaa aactatactc
     3601 tctactatat ccatagcatc tttagcagtc tctgatgtat atacattaaa acctatatta
     3661 gaaaaataaa tttgatttat gtttaaaata tcaacatcat catctacaac cagtaaatta
     3721 tacattacac cactccattc aaaggtattt taattataat tccaagtttt atagttatta
     3781 caaattataa tgttttattt tcatatataa ctggtattat tattttataa tatctttaca
     3841 aactagacta taatacttaa gtatttttga ataattcttg aatattgtat taaacattaa
     3901 gattcattat taaatactaa ttaaagtctc cgattgttgg agaatggagc ttaaagtcat
     3961 tttcacgtat aaatttatta tttttctaga aacttgtcta ttggctagtt tgagtattga
     4021 tttatatgat ttatatggtt ataatataaa ataaattgta ttattttggt aaatatattg
     4081 ttacaaaagg tttatattct gcatctcaat gtttataaaa actttatttt cttattataa
     4141 tactattatt cttttaaaaa aatttccctt ttccaacaaa tggctacttt aaaagccaaa
     4201 attttaccta tcaataaaaa tcttaacgac taatactctt attttcaaat tttgcaacat
     4261 atttgttaat tttaaaacaa aaaaagacta tgcaatataa aaattatatt catagccggt
     4321 tgcaccacta actccatata tctctagatg cccaataata agatatactt ctcagtttct
     4381 aaaatatttt tattaaaaat cttttatata ttttcataaa tttctttttt attctaacat
     4441 tgtaatatta ttttgtcaat atttccatat tcttagagat aagatactaa aatacctatt
     4501 ttatctaaag atatttatat ttctacattc aataatagac aataccctgc aagttaaagc
     4561 atcattttta tgagcttatc tcacagggta ttatatgttt actttatatt ataactcttg
     4621 ttattgatta actttaataa atcctttttc tgaaacaagt gcttgtcctt catcacttaa
     4681 tatataatct attaaagctt tacctttttc tgacaaacca tcttctttat taactaatat
     4741 aaatggtctt gatattggat atttattagc tattacattt gcatcagttg cttcaacatc
     4801 atttattttt aaactattta ctttctcatc aacatatcca aatgatatat atcctatagc
     4861 attttcattt ccttcaactg tagtttttat atttcctgaa ccatcactta tttgtgcatc
     4921 tttagttagt tcttctgaat caaaccctac aatttcttgg aatccatctc tagttcctga
     4981 accttcttct cttgatacta ttactattgg tgcatcattt cctcctactt ctttccagtt
     5041 agtaatcttt cctgtatata tacctttaac ttggtccatt gtcagatttt ttactgagtt
     5101 tgcagtatta gttatcaatg ctataccatc tattgctatt ttagtttcct ttaatccagc
     5161 acttttttct tcatctttta aatctcttga actcattcct atttcagatg ttccttctat
     5221 agcatttttt atacctccag aagaaccaat ctgttgtact tctacactaa caccttgatt
     5281 tttttcttga aacttttctc cgatagtttc cataagagga cctattgaag ttgaacctga
     5341 aacagaaact ttgcttgaat ctcctgaatc acctgaacct cctgagttag aactacatcc
     5401 tactgctaaa gctccaacta acattgtact agctaaaaga acgcctattt tctttttaaa
     5461 cattttactt acccccgctt atattttaat tttttgttgt catttttcta tattttaata
     5521 atacagattc tttgttaaat gaatattaga gcagggttaa gtaaatgtaa atattgttaa
     5581 atacatgtaa aaacttaatt ttatatgatt tattcttaat tagattaact ttttaaagtt
     5641 actttaggca gatataaaca taatacaact catattatca gaagtcttca ttgaagctat
     5701 caacctatca catcctataa ttctttttct agtttattaa ctaattcagc aaatacattt
     5761 aaggcttcct ctatagattc ttttttcgtc atatctacac cagcagattt taattgttct
     5821 agtgggtatt ctgaccctcc actttttaag aagtttatat atctatctac tgcagtactt
     5881 ccttctgaaa gtatctgttg acttaaagca cttgcagcag aaaaccctgt ggcatactta
     5941 tatacataga agtttgaata aaaatgaggt attctcgccc attctattcc tatttgttta
     6001 tctactattg cagattttcc gtaatacttt tcatttaatt tgtagtatat attagtaaat
     6061 tcatcagcag taagtggttc tccactctct accctttgat gtacaatttt ttcaaattca
     6121 gcaaacatag tttgtctatg aactgtagtt ctaaattgtt ctaaataata atttaataag
     6181 tatatagttt catccttaga cttagaattt tctaataaat aatttataag aagtaattca
     6241 tttaatgtag atgcaacttc tgcaacgaat atcttatagt ccgaatataa aaatggttga
     6301 cttgttcttg aataatgact gtgaacagag tgccctaatt catgaattaa agtgaataga
     6361 gaattaaggt catttttata gctcattaat atataaggat gtgaatcata acttccccaa
     6421 gaataagctc ctcctttttt accttcatta tcatatacat caatccaccc ttcatcaaaa
     6481 gcacttttta tgaccttcaa gtattcttct cctaaaggtt taagtgcctc taatatgata
     6541 tcctgtgctt tatcatatgg tatatccatg tcaaatttat ctgtcaaagg aacatacaaa
     6601 tcatacatgt gtatttcatt caatcctaaa aatttctttt taagctcaac atatttattt
     6661 agattaggaa tacttttatc tatagctgat ataagattat tgtaaacatc taaacttaca
     6721 tcatctgaaa ataaagacgc ttctattgct gaattatact ttctagtttt tgcgtaaaat
     6781 atctctgatt ttattccacc ataaagcgtt gaggcaaatg tatttttata tttctcatat
     6841 gttgaaaatt caccttcaaa agcgtctttt ctaactctac tattcttact ttttataaat
     6901 aaagagtagt ttgaatgaga aagcttaacc ttattcccct cctcatcttc tatctcagga
     6961 aattccatat ctgcatatgc taacatatcg tatacatttt ctggaattgt cgataattct
     7021 gatgtagctg caagtatttc ttcctctcgt tcactcaaag tatgtggttt atctctcaat
     7081 atatccttta tatattttgc ataaaaggat agcttctcat cttttaagta ttcacttaac
     7141 ttattctctt ctatcgccag tatttctgga actatataag aagtagccac acctaaatca
     7201 gtagataaca tttctgtctt agtggctctt ccttgattat cattaattct agtatcttca
     7261 tgttgtttca tatgagtata tacatataaa ttttgaacaa tcctagatgc attttcagag
     7321 atatttaata ctttatataa gttctcctta ctctcagaaa gcttcccttt atattctttt
     7381 acttcttgga taagattttt tactttactt atgtcttttt caatttcctc tttactggag
     7441 tacatcttat ctatcttcca tttgtacttt tcttctattg tacttctatc agttcccata
     7501 tcatacacct ctattttaat ttgttttata ttttattcac tttacaaaat atattgtata
     7561 tattttagtc caaataatct acatttatta ctccttctat catatttatt tctttcataa
     7621 cgtcgtgtaa gtgtattctc ttagcataag atacaaaaaa attaaccttc cagtattctt
     7681 cattacgaca ttctatttcc atatttctta cgataagttg atttttttca aatttatcat
     7741 ataaaaaagg tatagaatcc aaattgctta attttaatat caatacattc ttttcttggc
     7801 taaataaaaa cgtattgatt atcctaagca caaataatgc tatcattaca aataatccac
     7861 ctagaattgc caattcataa taaccatatc caactgctat accaatacat gcagttgccc
     7921 acatcccagc agctgtagta agccctttta ctccaccttt attttttaaa attgctcctg
     7981 cacctaaaaa accaattcca gataatactt gagcaggcat tcttgctggg tcagcatttg
     8041 tatacaaatt ataatcaaag aataattgaa tcgaagttat tgaaacaata caagaaccta
     8101 ttgcaattag tatatgtgtc ctaaatcctg caaactgtct tattttttct ctctctaagc
     8161 ctattattcc tcctattatt agtgctaata gtaaccttat tgctatctcc ttacttccta
     8221 tatcaatcat aatataaaac cctcctgact taattacaac ttatgtccta cagataattt
     8281 acacatcaga aatactattt tatatcttaa tattattatt aatatatgta aaatatagcc
     8341 atctgttaat ttttgataca aaaaaaccca caactgtggg ctatttgctt aggtacatgt
     8401 atttatttat taatatatct attttcttac ttgattctac taactcttct tgaaatattt
     8461 ctggatattc tatgtatgta tttatctctt ccctaactgc ttcaatctca ttttttaacg
     8521 cttctaattc attgttcgcc ataataatca ccactattac aaatttgtat atttttatat
     8581 acattttatc aaacaatatt atattttgtc aatattatgt caataaaact tactataaaa
     8641 atgctttaat ttaggtattg tatacctgga tactaagcct acaaacatac cagttactaa
     8701 tgatgccatt aacataaatg gtaagtaccc tatagcaagt atattttcta tgattaaact
     8761 tgcaactatt aattgaccta tattatgccc tattgcacct ataatgctta tccctattag
     8821 tgataaatct tttattttac tcgctaaata cataaataaa agactcagat aagctcctcc
     8881 tatactaaat agtagatatg ataaagggcc tgcaaatatt gatgacaaaa ttaccctgat
     8941 agtaacaatt ataacagtat ctttgacacc taacaacatt aatgaaatta aagttataat
     9001 attgctcaaa cccaattttg cccctggaaa tataactata agaggatttg gtatatatgt
     9061 ctctaataca tataaagcta aactataccc tatcataaga cctaaaaatg tcatttttct
     9121 agttttcatt aagttaactc ctccaaattt tatctatcat cttacattat aatacaaaat
     9181 atataaaaac acctaattta gactaaaata tttttaatct atttagaata aatattagtt
     9241 atttcaatgt gcaattacct ctataaatct tgactttttg tatcatactt gttacaattt
     9301 taatgataat gaatttcatt aggaggtgtt aataatgact gtttacgatt taaaaatagg
     9361 agaaaaagga tttattaata gtatagatgg agatattaaa ttatctaaaa gattgcttgc
     9421 acttggatgt ataaatagta ctgaaataga agttaaaaag gttgccccac ttggagaccc
     9481 aattataatt agatttagag gctttgacct tgcgattaga aaatctgatg ctaaaaatat
     9541 ttctttacaa taatagagga ggagctaccc gtgattaatg tagcattatt aggaaaccca
     9601 aatgtcggta aaactactgt ttttaatcta cttacaggtt caaatcaata tgtaggtaac
     9661 tggcctggtg ttactataga aaaaaaagaa ggatttcttg gaaaagagat aaaggttgtc
     9721 gatttacctg gtatatatgc aatggatact ttctcaaatg aagaaaaggt ttctaaatca
     9781 tatttagaaa atgaagatgt tgatgtaata gtaaatgtag ttgatgcttc taatttatca
     9841 agaaaccttt acttaacaac tcaacttatg caatttaaca agcctatagt tatattgctt
     9901 aatatgttag acattgcaga atctaaagga gttaatatag atgccaaaaa attaagtgaa
     9961 gaacttggag taattgttgt tccaattata gcaaaaaaga aaaatggtgt tgataggata
    10021 gaagaagaga ttaagggagc tacgcaaaaa gcttttatat atcagaaaaa ctttggaagt
    10081 gaaacagaaa catataaaaa attagaatcc attttatcga gatgtacaaa aactacatat
    10141 acacaaaaga aatctatcag tgataagata gacaatatag tcttgaatcc tatattagca
    10201 tatccgatat ttattggtgt attgttcctc ttatttaaat ttacctttga ctgggtcgga
    10261 ggaccactac aagagggatt tgctagtctt atagaggctt atatttctgc acctgttagt
    10321 gatatgttat ctaactctag cccatggttt aaatcactta tagttgatgg aataattgga
    10381 ggtgttggtg gtactttacc attcttccct ttaatattta cattattctt tggtatatcg
    10441 ttgtttgaag atagtggata tatgtcaaga acagcatttt taatggataa agttatgaga
    10501 aaggttggtc tatctggtaa agcttttata cctatagtta tgggtatggg atgttcttct
    10561 ccagctataa tggcaactag aacattggaa agcgaaaaag atagaagagt tacagctctt
    10621 attgcaccac ttatgacttg tggagcaaag ctacctatat atgcactatt tgtagctata
    10681 ttctttccac ataatgctgc tttagtaact acatcactgt atttggtggg tatagttatg
    10741 gcaattgtag ttgcattatt cttaaataaa acagccttta aaacagaggc tgaaccattt
    10801 atattagaat taccagaata tagaatccct actatagatg cacttattaa aaatacttgg
    10861 aataaatcaa aaggtttctt gataagagtt gttactgtca tgtttgcaat gtcagtcgta
    10921 atttggggac tttcttcatt caatgttttt ggatatacag aagatataaa tgtaagcttc
    10981 ttggctactc taggtcatat tatatctcct atattcaaac ctcttggttt tgatgattgg
    11041 agaacttcag ttgctatttt aagtggtctt ggagcaaaag aaatcgtagt aaactcttta
    11101 aatatactat atggtaatct aactgttgtc ttaccaacaa tatttaatgg agttactgca
    11161 tatacattct tgatatttac tgctttatat actccatgta tagctgcact tgcaactatg
    11221 aaaaaagaat atggcaataa aatgatgttt acatcatttg catatcaatt tatactagca
    11281 tggataatgg cttttatagt taaaagtata ggtggagtac tatttatggg taattcaata
    11341 atagaagctt tgataggtgg aataattata gtccttgcac tctttattct gtttaataaa
    11401 ttcaagtcta caaaaaacgg aaatgtctgt agtggatgtt ctatttgttc tggatgtcca
    11461 agtgctaatt cttgctcaag taaggaagac agcaataaaa aaaataatgc acactaatat
    11521 ataaatattt tagtttaaaa taagataaaa gtgggtgtct caaaattaac tttttagttc
    11581 atgaggcact cttttttata gaaaatcaaa tatattttca tcatttcaac agtaaaaaag
    11641 agcttatcta gtaaaaaaga ggcttatctc tattttgaga caacctcttt ttttgttact
    11701 tattcagttt cttgctacct aacttcatgt ggaacctagt atcttttgga acacttgggt
    11761 ctacacctct taatgtaggt atttccccac atattagttg tataggtact ataaaattaa
    11821 atgtttgata gtaaggatta tctgtgatat caatctttaa atttttatca ttttctgtaa
    11881 catctctacc tatagcaaat acattttccg tccaaccaga aagtacatca atcatcttag
    11941 ttactctagg ttcttttcct gtatctagta taaatatagt agaatcacta tttatagcat
    12001 tatatatacc atgtataaac tcttcaaact catacccagt tacaggtatt ctcattgttt
    12061 ccaataattt taatgctgct tctaatgtat ctccatatat atcagagtga cctattattc
    12121 ttatttcttt agaattaact aacttttctt tgtttctttc aatccattgt ttagagagct
    12181 tatacactgc ttcaaatcta ttaattgcgt ctaatatctt atttatttcc tcattgtact
    12241 tttcactaga tattatacct ttttctctag ctatttgaag acctaataac attaagttta
    12301 actttgtaca ataatatcct tttgttttag ctccagattt ttcttcacca caatttactg
    12361 ttaatatata atcagaaatc tcatctatta aagcattctt acaacctgcc atagatgcta
    12421 ttttacagcc cttatcttca gctaacttca tagcgttgta tgtagaataa ctactcccac
    12481 cttgagatac acctactact agggtatttt cattatcaaa cttgaatgta tcttcagtta
    12541 tcataaatgg atacattttc acaacatcta tatctaataa attttgtaaa tatggttgta
    12601 cttgcactcc tgagtgatag ctagtacctg aacctgttat tataatcttt tttagatttg
    12661 ttctcttaac ttcattaaat aagctatctg cattagatat aatttctctc attctaactg
    12721 gtgtttctaa catataatct tgtattgtca ttttatacac cttccttatc tatatattca
    12781 ataaattata attaatttct ataacaaacc tatagcatgt aaagcgattc ctgttactat
    12841 agtgcctata gttaattttg ttacacttac ctttctcttt aacatgtagt atattacaaa
    12901 tgcaaaggtt aaaggtaaca tattaggtat tattttatct agtatatctt gtaaaattac
    12961 ttctgcacca tttaagttga atttcagagg agtagttata ctcaacatag ttgcaaccat
    13021 tcctcctaca acgaataaac caacagttgt agccatagac attattttct ccataagtcc
    13081 ctctctttgt attctttcaa gagaatttac ccctacttga taaccatact taagtccaaa
    13141 tattctaagg gcaaatgctg gtatattgta taatagtaag aataatagtg gtccaaagat
    13201 attaccttct tttgcaagtg atactcctac tcctgctgct ataatcctaa atgttcccca
    13261 gaataatgaa tcaccaattc ctgcaagagg tcccataaga gctgttttaa cagcactaat
    13321 tgaactttca tcaaattctg gattattagc attctcttct tccatagctg ctgttatacc
    13381 taatatagca ggaactacta ctgtcgttgt attaaaaatc tccaaatgcc tttctattgc
    13441 ttttgcttga ttttctttct tattgtatag cttctttatt gcaggtaaca ttgaataaca
    13501 gtaacctaag ttttgcattc tttcgtaatt aaatgcacct tgaagagcaa atgaacgcca
    13561 aaacatactt cttaattctt tttttgttaa tttattcaaa gtcaatctct ccttccgtta
    13621 aagtattgat tgctaattga ggattttgat tattttcttt gttattgata taaaagttta
    13681 gtacaaatgc tacacatact ccaaataaag ctatagcagt tatatctaat ccaccataag
    13741 atgctaacaa aaaccctata aagaaaaata cagacatctt ttttgaaagt aacatatcca
    13801 taagcaatgc aaaacctact gctggcaaca atgttcctgc aacacccagt ccgtctaata
    13861 taactttagg tattgcattt atcaatgctg taactttatc agcaccaatc ataattgcta
    13921 aaaaggttgg aactgatgta cttaaaaaga ataatattac tggcaaccac atcattatgg
    13981 taactttctt aaaatcagct tgtttagcat agaaaactgc cttttgagaa aagtacgaat
    14041 ttattattct aactaatact ccaagagatt gtgctaagac tgctactggt actgctactg
    14101 ctaaagcaac ttctgcacca ttacctgata atatagcaaa agctgtacct aatacacctc
    14161 ctgttactac atctggagga gtagctgcac ctattcctgc aattcccatc caaattaatt
    14221 caagctgtgc accaatcata attcctgatt ttatgtctcc taaaactaat cctactaaca
    14281 tacctgtaac tattggccta tcaagcatag attgaccaaa tatacgccca tctagtatac
    14341 caactcctgc tataatagct aacatagtag cttgtactaa catattgaac ctccatttta
    14401 acttagttta atttacatta aattacatta catttttgat tttgttttga ttttctaata
    14461 actcatttac ataaacaatt ccattttttg cttcctctaa tcctgattta actattagtt
    14521 ttgtatcctc ctcaactaat aaaagtatag ataaaagcat tggtaaattt actcctgtca
    14581 ataattcaaa tttgaaccct tgtaaaagta agtttgtaca tatgtttgcc acacttccac
    14641 caaaaacatc tgttaagaca acaaattctt cactatcttt attatttgat aatatacttc
    14701 taatttcttt ctcagcatca tctgctgatt tatccgttgt catacaaaaa tactcaatat
    14761 tagttaatga accaattatt aatttacttg tgtcaacaag ctctctggac aattctccat
    14821 gtgttgcaat tagaaatttt ttcattctct ttctcctaaa ttattttatt aaatctgata
    14881 gttgtttctt tttgtctcta ggtaacattc taaattctat atcaatccct aaatcaccta
    14941 tttgtttaaa gttatttata tcatcatcat caacagcaac taaatctgta agtttcttct
    15001 tcccttcctc atatcttaat cctcctacat ttatttcttt taatactcct cctgaatttt
    15061 tagctacaac taaagcatct ttggtatttt ttactaacac taatacattg cttttctttg
    15121 aagaagcaaa tttttctact atttctccag attcctctac cccccttata tataaagtta
    15181 cattgctagg tttcgctaag ttcagtgaca tagctttcat ttggtcattt tttgcttcat
    15241 catttgcaac tagtattaca tttataccta gtgatgttgc ccatccatat gcaacctgac
    15301 catgtatcaa tctgtcatca attctcatca aagataccat tttccccaaa tcctccatca
    15361 tacattaaaa tttttattgt ttatactctc atttatctat aaaagcaaaa agtatgccaa
    15421 atactggcat acttttttaa atttcattca cttattttgt aatcttgtat tttactgtgg
    15481 tagaccatag ataatattat aaatatatcc tatctctgaa attggtattt gcacactata
    15541 actcttttct agcacactaa atgcattttt tattagtttt acttcactat gatggttttt
    15601 gattaacaaa tccatatcag tatactcttc aatttcagtt tgtctaacta atctttcaat
    15661 catacaactt aagtgaacat ataaagaaat ctttttatca tttgaaagtt ttttcttttg
    15721 aatattttct atagcattaa ttccttcttc tattctctct attatctttt ttgaatctaa
    15781 aatagttaga ctatctatta ctctaattaa tgaaaaattc tttacaatag aatcatttat
    15841 gttctttatt ttttcatttt ctactactct gctaaatatt ctaaaaacat catcttctcc
    15901 ttttcctgat attatatcct ctaataatat aaaatctacc ccattcacat ttggattggc
    15961 agttcctact atagccatca catcatacat ttcaaaaact ggttctttat ctcccataga
    16021 aacaagacta tcataatcat aaggtattat tttgacatca actattccaa ttaaactttc
    16081 ggaaagtaga tgttggattt ttatagcagt acctatccct gtaaaacaag atgatattat
    16141 agcatattct ttattttttt gaggataata caattgtata ttaggtttag tttcttcatc
    16201 cataatttct gcaatctctt caatgttttt accttgcatt aataaatttc ctacttctag
    16261 tgcagaaata gtggatattt ggtctataaa taacattggg ctatttatct tattctttaa
    16321 atcttcatat ataagattaa gagagcccat atcaaataac acaattaaac cattctttat
    16381 cctatctccc tctaggtatc tagacatttg ttttgataca tccgcaatag tagcatctat
    16441 aggcatatca atagctgtaa atatattttt acccaacatc cgattacata cattcgccat
    16501 actactggca gtagcatatc catgtgctat aatagtcgca ttaacttgat taaatttcat
    16561 tcttataatt gttgatttaa taaatataat cattatgatt atatctacat tattaagata
    16621 catatccaat ctattcgtta ttaatgttga tattttactt attatagagt attctttgta
    16681 actctctttt tttaaatata ttagtaattt attttctaaa gctttatact catcagacaa
    16741 ttgataagat tcgtccttca aataaagata agacgaaaat gccaatacat tattcccatc
    16801 atattttata ttgttattaa cttctaaatt tctaaatata tcctgcatta tcgatattat
    16861 aagctccata agtacgttat tattttcatc attatttgaa aagactaaat tgtccattag
    16921 tctaatcatt agattagaac tctttttact aaatttttcc tttgttattt catttttttc
    16981 tagtctatca aagtaatcta aacattttat gaaactttga tagattatat ttcttttatt
    17041 tcttccattt gacatttcaa tactaaattg tttaggagaa aagataatgc ttttgctttt
    17101 accactttgg aattgaaata tatctacatt ttctatataa agatttctag gaaggtcttg
    17161 aagagtaaca aaaatatttc catttcttct ttgatttttt gcataagctc gtccacatgc
    17221 atattttatt acatttttac attcacctat attccccttg aattttatct ttaaaatcgc
    17281 atttaatgta atttgagtta tctctatagg caaaccaagt accttacttt cttcataaat
    17341 aaaatgttct acaatctttc ttttttcaat agtccctctt tcttctatat tgggaacata
    17401 aatatttatg ggtattcttc ttaaaaaggt ttgtaaaaat tcagatagac tttctgttgt
    17461 agcaaatatt aatcttaccc tagcttttct aattactcca ttctctccaa tcctagaaaa
    17521 cgtacctgta tccataaatg taaataactt ttcctgacct tcactattta atctatgtac
    17581 ttcatctaaa aataaaacac caccatctga ttgttctatt agacctgctc tagattcatt
    17641 tgctccagta tatgctcctt ttacatgccc aaataataga ctagacaata actccaaatt
    17701 attataatac tgcgcacagt ttaacacatt gtatggagaa tttttttcta atacattact
    17761 gcttacacta tatttgtata ttatcttggc taaaaaagtt tttcctactc cactttcacc
    17821 tgtcagcatt attgtaagtc cgccatttgg ataaagtaca gcggttttta tttgttcgat
    17881 tacttctttt aagctattat catatcctat gatactatta aacacagtct cttgctttaa
    17941 taatttacac tcatttttaa gttcatctaa attttcataa atactttttt ttgtaggtcc
    18001 tactacatca tttattttat ttttaggtaa aaaaataaca ggtctactgt ttatctttat
    18061 aagattatca gattttacta aatcatttaa taatctactt gattgagttc tacttaaatt
    18121 aaactttttt actatatctt ctgttattat gccactttct tttttaagtt catcaaagtt
    18181 gttaaataat acacattcat ttacaaaact taaaatttca tccctcatgt tcaacctcca
    18241 atacaaatat caataatact ataatattag tatatcattt atatatttat gaaaaagata
    18301 attatattcc tatcttgatt ctcttttgta agtttgtaaa atcaaacttt ttataagtat
    18361 aaaatctaga ttttttaaca taatctcaag gttcttatct ttccatatat ctgacataga
    18421 atttatataa gaatatatt
//
LOCUS       DAMJYR010000069        18357 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.069, whole genome shotgun sequence.
ACCESSION   DAMJYR010000069 DAMJYR010000000
VERSION     DAMJYR010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 18357)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18357)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18357
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.069"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            166..1569
                     /locus_tag="PC471_002340"
     CDS             166..1569
                     /locus_tag="PC471_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDF2789467.1"
                     /translation="MKMLNNFFKKINNPAPVFAQTKMERDNQTDDSLSTKPKTTFRDV
                     AGLDEVKEELFEIVDFMKSPQKYQKMGAKIPKGVLFYGPPGTGKTLLASAVAGETNSS
                     FFNVTGSEFVEKYVGVGAKRVRTLFEKARKEAPSIIFIDEIDAVGAKRHLESNNEKDQ
                     TLNQLLVEMDGFNKDSNVLIIGATNRLDLLDEALLRPGRFDRHIHIGAPNYHTRFEIL
                     KVHTDDKPIDKSVNLELLAKKTHGFNGAHLSNIANEAAIFAVRDDSECITSEHFDKAL
                     ERVIAGLESKNSALVEKEKKIVAYHEAGHALVSDIVGICPIQKISIVPRGQALGYVLQ
                     LPDEDRYIYTKDELIGKIKILLAGKASEELIFNHKSTGAKDDLKKVTEIANQMVCEYG
                     MSNLGFMTIDGNDKTFLCDKVQKEANRIVEICYKETLEMLKDNLEDLHSVSKFLFEKE
                     TMTHEELKDLIGKEAVN"
     gene            complement(2046..3068)
                     /locus_tag="PC471_002341"
     CDS             complement(2046..3068)
                     /locus_tag="PC471_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDF2789468.1"
                     /translation="MNQKKVHYNKSATSKDVARLAGVSQSSVSRAFGSANGKGVKPEV
                     RERIFRVANEIGYVPNLVARGMISGKTNVIGLIVGDSLGPFYNRIINLFVEKIQEIGK
                     QCLVFKVPRQQSIDSIISKVIQFQVEAVIITASAMNKVMAETCEENNIPVILFNRFIP
                     GIKISTVYVDPIEGGGMAAEYLLKKGHKNIGYIQFTRETSEEMEKKIGFYSKLRQSGI
                     HNLKEESANYDYDSGYEAGKRMLALSNPPTAIFCTSDLIAIGVMDAARFEYKLRVPED
                     LSVIGYDDIQMASWKSYNLTTIRQPLDLLIEKTINILKSLLNEENPESVVEMLKPELI
                     ERGSTL"
     gene            complement(3103..4701)
                     /locus_tag="PC471_002342"
     CDS             complement(3103..4701)
                     /locus_tag="PC471_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HDF2789469.1"
                     /translation="MISSIDVCIIIGYLLLMLVIGYYSGKDNKNQEDYFLAGRSMPWI
                     PIALSVAATMISANGFIGGPGWAYVDGMYPVMVNITVPLAIFFALSITTPVIYKLKIT
                     SIYEYMGLRLGNYSRGLTIIQFFINSLIQASSMVYIPVLIIQMITGWSLYALVPIVVL
                     VSIIYTLLGGIKAVIWTDSIQMIVVISAVFIVIIVALKGMNLSFFDTLQIAQQSGKLN
                     TFDFSRDISVTNTFWATLIGGTVMWIRYFCFDQSQVQRVLTSKSLKCAKNSFVVSAFV
                     MNLVYYFMLLVGVILFVFYGGRTFETSNEIMITFILNELPVGAIGIIIAGVFAAAMSS
                     IDSILNSMTTVFTKDIYEFYFKKDNKESSLKVTMIITVVIGVVMTGFIIMGFGGSIKS
                     ILDLVGNYISYFAGPATGAFVLAMFTYKANDKGVSIGFIVGLVLGFFISNKYNVSWLW
                     NPAIGATITLIFGYIFSVILKKNHNADDIKKYTAFGMRNHLISQGMDKVDGVSIVPFS
                     LGKQELIVLAFFIIQYIILFLIQF"
     gene            complement(4743..6008)
                     /locus_tag="PC471_002343"
     CDS             complement(4743..6008)
                     /locus_tag="PC471_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDF2789470.1"
                     /translation="MKTTVEIENDIILGKDYSNLKRGIAFFSIALIYFFYCYNFMVGT
                     FIKPTMIYALADGGFGFSLKQTEEIFAVMSFGTIPGTFIFGVISTKIGKKRTLISVAL
                     LIGLTTFIPMLSPTNIMLWKIARLCTGVVLGGVFGTAMPLVADMFPSKYRGKLAAILT
                     ALFSLAMIFGGKVYGVLGDANWQILMYTAIIPPIVGAILAIFFVPDDLEYTKELVKKG
                     KETGEKISYISMYKGKYLWIGLGTILLSGANFTAYSAFSNNATTYLVTGIGMSAAVAE
                     SIYSLQGIGQLVGYLFWGSIADKFGRKIPFIGMALAAVFVFIYTKLGGDNTTTFYMIS
                     VLLGVAVGYSGAWGAYYTELFPSKYRSLSSGMSFNGGRIISTFAIPAIAGTASGTLGM
                     MTIFYISIAVFVAGAIVWLFLPETLNNKK"
     gene            complement(6150..7577)
                     /locus_tag="PC471_002344"
     CDS             complement(6150..7577)
                     /locus_tag="PC471_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HDF2789471.1"
                     /translation="MASTNKNNATSKEEEKEVSTSGLESCTVKENKAKMNQTNFSNIN
                     ENQTNENKTNTYKRNIDKGNIDKGNIDKTNTNKSDINKVNIDTVDTDKLNTNTLTEKN
                     KKVIFKGEDNMSSTKIYYGDSLEVTPVGVMDYNDFSKQTKREQEIPGFDSKYRDFVDY
                     IMKITHNIWEEKGIGVIYDTYHNNVTMHCGSSNLVGIKDVISNTLQTLHAFPDRRLIG
                     QNVIWSNFGADGFLSSHRVLSTATNLGDSNFGPATGRKINFRTVIDCATTNNRIHEEW
                     LVRDNLWIVTQLGLNPQEVAKSMAKASESKVLSLQSTYGICESMDGQFMPTKYQAKDD
                     SVGEMMLEMTSRIYNYKYINEVKKYYHDNAVVHFICDKDLNGYDEIQGMIVSLFASIP
                     NGSYEVERVTCNQREKNEGYDVSVRWRLRGINEGIGFFGQPSGKHIEVMGINHYHILQ
                     GKVKEEWITFDGMDVLKQIYMGVEE"
     gene            complement(7615..8622)
                     /locus_tag="PC471_002345"
     CDS             complement(7615..8622)
                     /locus_tag="PC471_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HDF2789472.1"
                     /translation="MGKYQDSKKVVRNYFEALENATGDEVENVLKQHMKSDYNWKGVY
                     PFREQEGTENVADVFWKPLKKSLSNMQRRQDIFIAGTNEIDNSEWVMSMGHFMGLFDN
                     DWLGIKHTRKMISLRYAEFNCVQDGKIAKTGLFVDIIGFMIQAGVNPLPPQTGSYFVY
                     PGPIDHNGLLFEDADEVEGVKTLALVNKMVDDLSELNESGAMGCPPEILEKSWAKDMI
                     WYGPGGIGASYTIPRYQEQHQLPFRNNLKGKTFNGHVCRFAEGNFACFFGWPNLTNVP
                     CGGFLGLPGGEVKADMQVVDVYYRKGDKLQENWVLIDIPYWLKQQGLDILERTKSIFN
                     A"
     gene            complement(8819..10141)
                     /locus_tag="PC471_002346"
     CDS             complement(8819..10141)
                     /locus_tag="PC471_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2789473.1"
                     /translation="MKILYVGPGMVIDKILAVSKKNFPNIEADFLRYDRYIEVPDLLE
                     KYRGRVDAVLFTGKSPFKFFEKRQKDFVLSDYIPRHETTLYRVLLEITYLLKFDISRL
                     SIDTYDKAMMKRLYKEIGVEFKDEHMFFAEQKYLEEDYIKFVLEFHKYNYQNNDVCCC
                     ITGLEEAYHIMKKDNIPVVLALPSEDVMVQSIKNIQMRYIAKKNNENQIIVLAIKLNM
                     PSEYSLMKEDEYAYLSQRIKILDKLYHFNSRIDGVLVEQSRSEFMIFTTKKILELETK
                     KYRDIYLLDMIREVSVINTYIGIGYGKTANEAKFNAYESIKMAQSQKNDAAYVVFENG
                     EIMGPLKPNPTVKEKNSFDERFYQIATETGLSANTVYKVFNKIINSGKTEFTSKELAT
                     ICGVSIRTMDRIILRLCDSGYCEVVSEQLMSKYGRPSRILRFKPFLLF"
     gene            complement(10499..12139)
                     /locus_tag="PC471_002347"
     CDS             complement(10499..12139)
                     /locus_tag="PC471_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacylase"
                     /protein_id="HDF2789474.1"
                     /translation="MFDVKIVNGIIVDGTGNSRYKSDVGIVGDKIMAIGDLSQKEAKE
                     TIDATGKFVSPGFIDFHTHSDLSLVYDKYTRSRIHTGVTTDVIANCGIGVAPIREEKK
                     QELIDYLGTRIIGTIPTKLELHWNTMQEYFDYLTENSPAVNVVAYVAQGPVRINEMGF
                     SKEPATLEQLKNMKLEVRKAMEAGCVALSSGLVYLPGAYTKKEEMAELCKELIPYNGY
                     YISHIRDEGDEEMEALDEAIYIAKTAGVPLHISHLKVMGHKNFGTIDEVFKKLDEAEA
                     DGLEVTFDCYPYTAGMTSLGALLPPWAFEGGVENMVKRLEVQENRDRIIKELEEGIPG
                     WQCFYQLAGGWNGVVLASVMTEANKYVEGKTLMEVAKINGENPFDTFFRLLIEEKSKI
                     QVVVHTQGQEDTDKVVCHPKSCIGSDSMDLSTEGLLSLGKPHPRAFGTFGRIFSYYVR
                     EKGMLTFEEAVKKITYLSAKRLGIYKERGLLKENYFADIVVFDPDTIEDKATYSNPKQ
                     YTVGVEYVLVNGKIALAGGKQTDVCAGRVIKNPLSIAK"
     gene            complement(12295..12951)
                     /locus_tag="PC471_002348"
     CDS             complement(12295..12951)
                     /locus_tag="PC471_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2789475.1"
                     /translation="MYDKKMIKIGRISIGLAIIANFIPAIYVGIRFGQMPMLSVIFQI
                     WGLVAATYGVSWIVQPIAYYPTLGAPGSYIGWLAGSVGDIRMPAASMAQKIAGVEAGT
                     HEGEVVGTIGTCCSIFVSASMITIFTIVGYKVIPLLPEFVTNSFNYILPALFGAIYVD
                     IARKDIRAGGCTIAVALAIMYFGEKIGIPGGLLTLFIVLGGILITRVFFVIDNNKTKE
                     "
     gene            complement(12977..13678)
                     /locus_tag="PC471_002349"
     CDS             complement(12977..13678)
                     /locus_tag="PC471_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5058 family protein"
                     /protein_id="HDF2789476.1"
                     /translation="MKFQEMINSPGLWIVSSFLVIISVVQAIVFMKEALKEASNLGIE
                     KKGIKAAIRSASVTAIGPSLSPVITLLSLVAVIGAPTTWMRLCDVGAARTELGVISLT
                     SNLSGVEVGSAAFGAEAFSYALWGMALNNLGWLFVVFILGHRMRGIVEKMNMKYNPTW
                     VKKLLAGATVGLFAYLLSNQIKTFEIPKLTPAIISAIVMLVLTTLFKKNQRLQELSLG
                     IAMLIGMFGTQIILS"
     gene            14486..14965
                     /locus_tag="PC471_002350"
     CDS             14486..14965
                     /locus_tag="PC471_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HDF2789477.1"
                     /translation="MDKTTFTNNILESEQTLYRVSKSILGNDQDCEDAVNNAILKAYE
                     KLDSLKEEQYFKTWLIRIVINECNSLRRKRLKSLSFEDVFKNKKIDEKDDYSDLYTAI
                     QSLPKKIKIPIVLYYIEGYSVDEVKEILDIPQGTVKSRLSRGRRLLKTKLENTEVII"
     gene            14962..15741
                     /locus_tag="PC471_002351"
     CDS             14962..15741
                     /locus_tag="PC471_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2789478.1"
                     /translation="MNKNILNNSFPEVPESFHNSLSKTLNSLPEREENYKMINNKTHK
                     LPFRKGLVATLAITLILSTTALAVGHIYSLVGKSSTKATYTSIPTTEQVKKDFNFTPN
                     IVSEFSNGYKFKGAYTVNKKAFDKENNVLGKSKALSFEYKNGNDEISLDTANNVLGKS
                     SDNLKVVDTYKDINISYEAFAQKFIPEGYKMTEQDKIDEKSGKYTFSVGPNNESDKVE
                     VNEFKFLTWKQDGVYYSFTVQDSNLSMNDLVKMAHEVIDAK"
     gene            complement(15991..16491)
                     /locus_tag="PC471_002352"
     CDS             complement(15991..16491)
                     /locus_tag="PC471_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDF2789479.1"
                     /translation="MIEGIKFIKAEEKYIYSYWQTFDKIAKERKYLAMDEAFPFEETV
                     EFIKNIINKNLPQLFIIDLESDNCIGWCDVLPKTEKVGYLGMGILKEYREKGIGSSLL
                     KQIIDLSKEYGYEKIELDVFKSNSRAIHVYKSLGFVEVNTISSGFTWNDRPVKEEVIQ
                     MELTLI"
     gene            complement(17103..17810)
                     /locus_tag="PC471_002353"
     CDS             complement(17103..17810)
                     /locus_tag="PC471_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HDF2789480.1"
                     /translation="MIFTKSNKYDKDFLMKNMMGPNCIKILEELTSKIKLEKGMRILD
                     LGCGKGISSIFLAKEFDATVFATDLWIEPTENYKRFKEFKLDDKIFPIQAEAHELPYA
                     EGFFDAVISIDSYHYFGNKEGFLDNHISPLVKEGGILAMAMPGLKEDFVDCIPDELIP
                     FWQDNMNFHSITWWNKLWSESESVIVEKCEALNCHDEAWKDWINCDNSYAINDKKMME
                     VENGKYFNTISLIARTK"
BASE COUNT         6352 a         3274 c         2128 g         6603 t
ORIGIN      
        1 aagtagaaat ctctatatac ataaattcat tttttcatac atcttacaaa tttattttta
       61 tttagataac atgtatattt tttccatgaa aaataaataa taaaaaatag taaatatgga
      121 aaatataaaa ttgattaaca ataattaatt acctgaggtg tgaatatgaa aatgctaaac
      181 aactttttta agaaaatcaa caatccagct cctgtctttg cacagacaaa aatggaaaga
      241 gataatcaaa cagatgattc actttctaca aaaccaaaga ctacatttag agacgttgct
      301 ggattagacg aagtaaaaga agaactattt gaaatagtcg attttatgaa gtcaccacaa
      361 aaatatcaaa aaatgggtgc aaaaatacct aaaggagtat tattttatgg tcctccagga
      421 acaggtaaaa ccctacttgc atctgctgta gctggtgaaa caaactcatc atttttcaat
      481 gttactggtt cagagttcgt tgaaaaatat gtaggtgtgg gagcaaaaag agttagaact
      541 ctatttgaaa aagctagaaa agaagctcca agtataatat ttatagacga aatagatgct
      601 gtaggagcaa agagacatct tgaaagtaac aacgaaaaag accaaacact aaaccaactt
      661 ttagttgaaa tggatggatt taataaagat tccaatgtat taataatagg tgctaccaat
      721 agacttgact tattagatga agctttactt agaccaggaa gatttgacag acacattcat
      781 ataggtgcac caaactatca cactagattt gaaattctaa aggtacatac agatgacaaa
      841 cctatagaca aatctgtaaa tctagaactt ttagctaaaa aaactcatgg tttcaatggg
      901 gctcatcttt caaatatagc aaatgaagca gctatatttg cagttagaga tgacagtgag
      961 tgtattacat cagaacattt tgacaaggca cttgaaagag ttattgcagg gctagaatct
     1021 aaaaactctg cattggttga aaaagagaaa aaaatagtag catatcatga agctggacat
     1081 gctcttgtaa gtgatatagt aggaatttgt cctatacaaa aaatatctat agttcctaga
     1141 ggacaagcat taggttatgt tcttcaatta cctgatgaag atagatatat ttatactaag
     1201 gatgagttga ttggaaaaat aaaaatatta cttgctggaa aggcttcaga agaattaata
     1261 tttaatcata aatctactgg tgcaaaggac gacttaaaga aagtcactga aatagctaac
     1321 caaatggttt gtgaatatgg tatgagtaac ttaggattta tgactattga tggaaatgat
     1381 aagactttct tatgtgataa ggttcaaaag gaagcaaatc gtatagttga aatttgttat
     1441 aaagagactt tagagatgtt aaaggataat cttgaagatt tacattctgt ttctaaattc
     1501 ttatttgaaa aagaaactat gactcatgaa gagttaaaag atttgatagg aaaagaagct
     1561 gtaaactaat atagttttaa aaatttgagg ctgtctcaaa atagagataa gcctcttttt
     1621 attgttaaag ttataaaaat acatttgatt ttatacaaaa aagtgcctca taaactaaaa
     1681 atttcatttt gagacactta catattttaa ttacatattt taattttgtc tatttacaga
     1741 atattatatc tacataaaac taaactgctg taaacttaac tacctccagt aatgtttgaa
     1801 gtggtataaa agctagtagc ctagtttcta cacttataat ttatatttta cataactaaa
     1861 tttagtagat ttttatactc acaatttaga acgattgaaa acttaaaaat ttcttatcag
     1921 atatctacaa cttaattagg tatctatcac ttgattaaat atctatcact taattaggta
     1981 tctatcactt gattaaatat ctgccattta attaaatatc taccacttaa ttaaatatct
     2041 accacttaaa gtgtagaacc tctttcaatc aattcaggtt ttaacatttc aacgactgac
     2101 tctggatttt cttcatttaa taaggactta agtatgttaa ttgttttttc aatcaacaaa
     2161 tctagtggct gtctaatcgt cgttaaatta taactcttcc atgaagccat ttgaatatca
     2221 tcatatccta tcactgataa atcctctggc accctcaact tgtactcaaa ccttgcagca
     2281 tccattacac caatagctat caagtcactt gtgcaaaaaa ttgctgttgg aggatttgat
     2341 aatgctaaca ttctcttacc tgcttcatat ccagaatcat aatcatagtt tgcagattct
     2401 tctttaaggt tatgaatccc actttgtctt aacttagagt aaaatccaat tttcttttcc
     2461 atttcttcac ttgtttctct cgtaaattga atatatccta tattcttatg acctttctta
     2521 agcagatatt cagcagccat tcccccacct tcaattgggt ctacatagac tgtacttatt
     2581 tttataccag gtataaaacg attaaaaaga atcacaggaa tattgttttc ttcacaagtc
     2641 tcagccatca ccttattcat ggccgaagct gtaataatca cagcttctac ttgaaactga
     2701 attacttttg aaattatact atctatactt tgttgtctag gaactttaaa aacgagacac
     2761 tgtttaccta tctcttgaat tttttctaca aataaattga taattctatt gtaaaatgga
     2821 cctagactat caccaactat caatcctata acattagttt ttccactaat cattccccgt
     2881 gctaccaaat taggaacata tcctatttca tttgctacac gaaaaattct ttctcttact
     2941 tcaggtttta cgccctttcc atttgcacta ccaaatgctc ttgaaacaga tgattgagat
     3001 actcctgcca atcttgcaac atcttttgag gtagccgatt tattataatg aactttcttt
     3061 tgattcattt agtatttctc cttaaatatc tagtatttct tcttaaaatt gtatcaaaaa
     3121 taatataata tattggataa taaaaaaagc taatacaatt aattcttgtt tacctaaaga
     3181 aaatggcact atagatactc catctacttt atccattcct tgagaaataa ggtgatttct
     3241 cataccaaat gcagtgtatt ttttaatatc atctgcatta tgatttttct ttaaaatgac
     3301 actaaaaata tatccaaata tcaatgttat tgtagcacct attgctggat tccacaacca
     3361 actcacatta tatttattag aaataaaaaa tcctaaaact aaccctacaa taaaaccaat
     3421 agatactcct ttatcattag ctttatatgt aaacattgct agtacaaatg cacctgtagc
     3481 tggtccagca aagtatgaga tatagttacc aacaaggtca agaattgatt tgatgcttcc
     3541 tccaaatccc ataataataa aacctgtcat aacaactcct ataactacag ttataatcat
     3601 tgtaactttt aatgatgatt ctttgttatc ttttttgaaa taaaactcat aaatatcctt
     3661 cgtaaaaaca gttgtcatac tgtttaatat agagtcgata ctagacattg ctgctgcaaa
     3721 cacacctgca ataataattc caatggctcc tactggaagt tcatttaaaa taaaagtaat
     3781 cattatttca tttgaagttt caaatgtcct tcccccataa aaaacaaata aaattactcc
     3841 taccaatagc ataaagtagt aaactaaatt cataacaaat gcactaacta caaaagaatt
     3901 ttttgcacat tttaaagatt tagatgttaa aactctttgt acttgagatt ggtcaaaaca
     3961 aaagtatctt atccacatta cagttccacc aattaaagta gcccaaaaag tatttgtcac
     4021 tgaaatatcc ctactgaaat caaatgtatt taattttcca ctctgttgtg caatttgtaa
     4081 tgtatcaaag aaacttagat tcattccttt taaggctaca ataattacta taaatacagc
     4141 acttattact actatcattt gaatactatc agtccatata actgccttaa tcccacctaa
     4201 tagagtgtat ataattgata caagtacaac tattgggact aaagcataaa gtgaccagcc
     4261 agtaatcatt tgaataataa gcactggaat atataccata gaactagctt gaatcaatga
     4321 attaataaaa aattgaataa tagttaatcc tcttgaataa tttcctaaac gaagtcccat
     4381 atattcatat atagaggtta tttttaattt atagataact ggggtggtaa tgctcagtgc
     4441 aaaaaatatt gctaaaggta ctgtaatatt aaccattact ggatacattc catctacata
     4501 tgcccatcca ggaccaccta taaagccatt tgcactaatc atagtagctg caactgataa
     4561 agcaattgga atccatggca ttgacctacc tgccaagaaa taatcttctt gatttttatt
     4621 atcttttcct gaataataac caataactaa cattaatagc aaatatccta ttataataca
     4681 tacatcaatt gatgaaatca taagccaccc ctttctaagt aacaaaataa attcctagta
     4741 ttttattttt tattatttaa cgtctctggt aagaataacc atacaatagc acctgctaca
     4801 aagactgcga ttgatatgta aaagatagtc atcataccta aagtaccaga agctgtacca
     4861 gctatagcag gtattgcaaa cgtagatata atccttcctc cattaaatga cataccagat
     4921 gacaacgaac gatatttact tgggaacaac tctgtgtagt atgctcccca tgctccagaa
     4981 tatccaacag caacacctag taatactgat atcatataga atgttgttgt attatctcca
     5041 cctaattttg tatatataaa aacaaatact gctgctaatg ccatacctat aaaaggtatt
     5101 tttcttccaa atttatctgc aattgaaccc cagaataaat atcctactaa ttgaccaatt
     5161 ccttgtaaac tatatataga ctcagccact gctgctgaca ttcctattcc tgtcactagg
     5221 tatgtagttg cattatttga aaatgctgaa tatgctgtaa aatttgcacc tgataaaaga
     5281 atcgttccca atccaatcca taagtatttt cccttataca tacttatata gctaatcttt
     5341 tcaccagttt cttttccctt tttaactaat tcctttgtat actccaaatc atctggcaca
     5401 aagaagattg ctaagatagc tccaactata ggtggtataa tcgcagtata catcaatatt
     5461 tgccaatttg catcacctaa aacgccatat actttcccac caaaaatcat tgctaatgaa
     5521 aataaagcag ttaatatagc agctaactta ccacgatatt tacttggaaa catatctgca
     5581 actaaaggca tagctgttcc aaatacgcct cccaacacta ctcctgtaca caaacgtgct
     5641 attttccata acataatatt tgtaggagat aacattggta taaatgttgt caatccaatc
     5701 agtaaagcaa cgctaattaa agtcctcttc ttcccaattt ttgtagaaat caccccaaaa
     5761 ataaatgtac ctggaattgt accaaaagac ataactgcaa atatttcttc tgtttgtttt
     5821 aatgaaaaac caaatccccc atctgctaaa gcataaatca ttgttggttt aataaaagta
     5881 cccaccataa aattgtagca ataaaaaaag tatattaaag caattgaaaa aaatgcaatt
     5941 cctcttttta ggtttgaata gtcttttcct aatatgatat cattttctat ttcaaccgtt
     6001 gttttcatat ttttataatt ccccctttta aatttagtca taattttgct aaaagtttaa
     6061 acatctattt ttcatataca ttccccactt aaattttata aagcaatact tccctatata
     6121 tttcaaaact atttatactc taaacagctt tattcttcta ctcccatata aatttgtttt
     6181 aatacatcca taccatcaaa agttatccac tcctccttta ctttaccttg tagtatatga
     6241 taatgattga ttcccattac ttctatatgc tttccagaag gctgtccaaa aaatcctata
     6301 ccttcattta ttccacgtaa tctccatctt acagatacat cataaccttc atttttttct
     6361 ctttgattac aagttactct ttccacttca taacttccat ttggaattga tgcaaataaa
     6421 cttacaatca ttccttgaat ctcatcatag ccatttaaat ctttatcaca tataaaatgt
     6481 actactgcat tatcatgata gtatttttta acttcattta tatatttata attataaata
     6541 cggcttgtca tttcaagcat catttcgcca acagaatcat cttttgcttg atacttagtt
     6601 ggcataaatt gaccatccat agattcacag ataccataag ttgattgtaa actaagcact
     6661 ttagattcac ttgcctttgc catactcttt gctacttcct gagggtttaa tcctaattga
     6721 gtaacaatcc ataaattatc tctaaccaac cattcttcat ggattctatt gttagttgtg
     6781 gcacaatcaa ttacagttct aaagtttatt tttctacctg ttgctggtcc aaaattacta
     6841 tcacctaagt tagttgctgt agacaaaaca cggtgagaag ataaaaaacc atcagcacca
     6901 aagttagacc atattacatt ttgaccaatt aatcttctat caggaaacgc atgtaatgtt
     6961 tgaagagtat tggaaataac atctttaata ccaactaaat ttgaagagcc acaatgcatt
     7021 gtaacattat tgtgatatgt atcataaata acacctatac ctttttcttc ccagatatta
     7081 tgtgtaatct tcataatata atctacaaaa tctctatact tagaatcaaa tccaggtatt
     7141 tcctgttccc tttttgtctg ttttgaaaaa tcattgtaat ccattactcc aacaggcgtt
     7201 acttctaatg aatctccata gtaaattttt gtagatgaca tattatcttc ccccttaaat
     7261 ataacttttt tatttttttc agtaagagta tttgtattta atttatctgt atccactgta
     7321 tctatgttaa ctttatttat gtcggattta tttgtgtttg ttttatctat attcccttta
     7381 tctatatttc ctttatctat atttctttta tatgtgttcg ttttattctc atttgtttga
     7441 ttttcattta tattactaaa attagtttga ttcattttag ctttgttttc tttaaccgta
     7501 catgattcaa gcccacttgt agaaacttct ttttcttcct ctttacttgt tgcattattt
     7561 ttgttggtag atgccacttt atatccccct ttgttttaga atacgtatgc ataactaagc
     7621 attaaaaata cttttagttc tctcaagaat atcaagccct tgttgtttca accaataagg
     7681 aatatcaatt aacacccagt tttcttgaag tttatcgccc ttacgataat aaacatcaac
     7741 aacttgcata tctgctttaa cctctccacc tggtaatcct aaaaaacctc cacatggtac
     7801 attagtcaga ttaggccatc caaagaaaca agcaaaatta ccttctgcaa atctacatac
     7861 atgcccatta aaagtctttc cttttaaatt atttctaaat ggtagctggt gttgctcttg
     7921 atatctaggt atagtataag aagcaccaat tccacctgga ccataccaaa tcatatcttt
     7981 agcccaagat ttttcaagta tttctggtgg gcatcccata gcaccacttt catttagttc
     8041 agataaatca tctaccattt tatttactaa tgctaaagtt tttactcctt caacttcatc
     8101 tgcatcttca aaaagtaatc cattatggtc aattggccct ggatatacaa aatatgaacc
     8161 tgtttgtggt ggcaatggat taactcctgc ttgaatcata aacccaatta tatctacaaa
     8221 taatccagtt ttagcaattt taccatcttg aacacagtta aattctgcat atcttaaact
     8281 aatcatttta cgagtatgtt taatgccaag ccaatcgtta tcaaaaagtc ccataaaatg
     8341 ccccatactc attacccatt cactattgtc aatctcattt gttcctgcaa tgaaaatatc
     8401 ttgtcgtctt tgcatattac tcaatgattt ttttaaaggc ttccaaaata catctgctac
     8461 attttcagtg ccttcttgtt cacgaaatgg atacacacct ttccagttat aatctgattt
     8521 catatgttgc tttaagacat tttcaacctc atccccagta gcattttcta atgcttcaaa
     8581 ataatttctt acaacttttt ttgaatcctg atattttccc ataattatct cctcccagat
     8641 ttatttaagt tataaattaa ctttctcatt tcatgcatac gatttccttt ttaatgattt
     8701 tttagctgtt ttaagtttat tttatgaata catatgcatt tttctaaata ttaacatata
     8761 tctaaaaaat tgtaaagaaa atgttatcat aaaaatttaa tttttgactt atatctattt
     8821 aaaacaacag aaaaggttta aaacgtaata ttctactggg tctaccatac ttgctcataa
     8881 gctgttcact cactacctca caataaccag aatcacacaa tctaagtata atcctgtcca
     8941 tagttctaat acttacacca catatagtag ctaattcctt tgatgtaaat tcagtttttc
     9001 cactatttat tattttattg aatactttat atacagtatt agcacttagc cctgtctctg
     9061 tagcaatttg ataaaaacgt tcatcaaaac tatttttctc tttgacagta ggatttggct
     9121 ttagtggtcc cataatttct ccattttcaa aaactacata tgctgcatca tttttttgac
     9181 tttgagccat cttaatactc tcataagcgt taaatttagc ttcatttgct gtttttccat
     9241 aaccaattcc tatataagtg tttataactg aaacttcacg aatcatatcc aacaaataaa
     9301 tatctctgta tttctttgtt tctaactcaa gtattttttt agttgtaaaa atcataaatt
     9361 cacttctact ctgctctacc aaaacaccat ctatacggct attgaaatgg tatagtttat
     9421 caagaatctt aatcctttgt gaaagataag cgtactcatc ttccttcatt agtgaatatt
     9481 ctgaaggcat gttcagttta atagcaagaa caataatctg attctcattg tttttctttg
     9541 caatataacg catctgaatg ttttttatac tttgcaccat tacgtcctca gaaggcaagg
     9601 ctaaaactac tggaatatta tcttttttca ttatatgata ggcctcttca agacctgtaa
     9661 tacaacaaca tacatcatta ttttgataat tatatttatg aaattcaagc acaaacttta
     9721 tatagtcctc ttctaaatac ttttgctctg caaaaaacat atgttcatcc ttaaattcaa
     9781 ctccaatttc tttatacaat ctcttcatca tagccttatc ataagtatct atactaagtc
     9841 tactaatatc gaatttaagc aaatatgtaa tttcaagcaa cactctgtac aatgttgttt
     9901 catgtctagg aatataatca gacaatacaa aatctttttg tcttttttca aaaaatttaa
     9961 atggcgactt acctgtaaaa agaactgcat ccactctacc tctatatttt tccaataaat
    10021 caggtacttc aatatatcta tcatatctaa gaaaatctgc ttcaatatta ggaaaattct
    10081 ttttacttac tgctagtatt ttatcgatta ccatacctgg tcctacataa agtattttca
    10141 tttcgtctct cctttcctaa tgatactatt atctcttaaa acattttcta aacctaccat
    10201 gactaaagcc acaggactcc tgattcatag aattttgaat aatcaattat acctaatata
    10261 aaaagttact ttaagttttt taattacgac tatctgttga aacatttttg tatttaaatt
    10321 tttgtattta aatcttattc attaatatta attacatttt acaattaata tattgttttt
    10381 tctacttaaa gttaaattct ttagaaaaca caagtagagt aatctttatt aatttaacaa
    10441 aaatagcctt ccttaatatt caaagaaaga ctatttttat aaaagttctt aatgtattct
    10501 attttgctat gctaagagga tttttaatta cacgtccagc acatacatca gtttgctttc
    10561 ctcctgccaa tgcaatttta ccattcacca acacatattc cactcccact gtatactgtt
    10621 taggattact ataagtcgct ttatcttcta ttgtatctgg gtcaaatacc acaatgtcag
    10681 caaaataatt ttctttaagt agaccccttt ctttataaat gcctaagcgt tttgctgaaa
    10741 gataagttat ctttttaact gcttcctcaa aagtcaacat acctttttct ctaacatagt
    10801 aagagaaaat tcgtccaaaa gtaccaaatg cacgaggatg aggctttcca agagaaagta
    10861 aaccttctgt agaaaggtcc atactatcag aacctataca tgactttgga tggcaaacaa
    10921 ccttatctgt atcctcttga ccttgtgtat gaacaactac ttgaatttta cttttttctt
    10981 caatcaaaag cctaaagaac gtatcaaatg gattttcacc attgattttg gctacctcca
    11041 ttaaagtttt accctctaca tatttatttg cttcagtcat tacagaggct agtacaacac
    11101 cgttccaccc acctgctaat tggtagaaac attgccatcc aggaatccct tcctccaact
    11161 cttttataat tctgtctcta ttttcttgta cttctaatcg ttttaccata ttttctacac
    11221 cgccttcaaa agcccaagga ggtaaaagtg cacccaaaga tgtcattcca gctgtatatg
    11281 ggtaacaatc aaaagttact tccaaaccat ctgcctcagc ttcatctagc tttttaaata
    11341 cctcatcaat agttccaaaa ttcttatgac ccataacttt taaatgagaa atatgtagag
    11401 gaactcctgc agtcttagct atataaattg cttcatcaag agcttccatt tcttcatcac
    11461 cttcatctct aatatgagat atatagtatc cattgtatgg tatcaattcc ttacataatt
    11521 cagccatttc ttctttcttt gtgtatgctc ctggtaaata aactaatcct gatgaaagag
    11581 ctacacatcc agcttccata gcttttctaa cttccaactt catattttta agttgctcta
    11641 aagtagctgg ttcctttgaa aatcccattt cattgatacg tacaggtcct tgtgctacat
    11701 aagctacaac attgactgct ggagaatttt cagttaagta atcaaagtat tcttgcattg
    11761 tattccaatg aagctctaat tttgttggaa ttgtaccaat tatcctagtt ccaagataat
    11821 caattaactc ttgcttcttc tcttctctta tcggagcaac tcctatacca cagtttgcaa
    11881 taacatctgt tgttacacct gtatggatac ggcttcttgt gtatttatca tacacaagag
    11941 ataaatcaga atgagtatgg aaatctataa aaccaggtga tacaaatttt ccagttgcgt
    12001 ctatagtctc tttagcctct ttttgactta aatctccaat agccatgatt ttgtctccta
    12061 caatccctac atcactttta tatcttgaat ttcctgtacc atcaacgatt attccattga
    12121 caattttaac atcaaacatg ctttttctcc tttcaagact tatttatatt catatttctt
    12181 aataaacaat cctattcttt tttctcaaca aataattcta gtctttctcc ttaataaaca
    12241 atcctattct ttttctcaac aaataattct agtctttctc cttaataagc aatcctattc
    12301 ttttgtttta ttgttatcaa ttacaaagaa gacccttgtg ataagtatcc cacctaacac
    12361 aataaacaat gttaataatc cacctggtat accaattttt tcaccaaagt acataattgc
    12421 taacgctaca gcaatcgtac atccaccagc tcgaatatcc ttacgtgcaa tatcaacata
    12481 aattgctcca aataatgctg gaagtatata attaaatgaa ttagtaacaa attcaggtaa
    12541 caaaggaatt actttatagc ctacaatggt aaaaatggta atcatagaag ctgatacaaa
    12601 gattgaacaa catgtaccaa ttgttccaac aacttcaccc tcatgagttc ctgcttctac
    12661 acctgcaatt ttctgtgcca ttgatgctgc aggcatacgt atatctccaa cagaacctgc
    12721 aagccaacct atataagaac ctggagctcc tagtgtagga taataagcta taggttgaac
    12781 aatccaacta accccataag tcgcagcgac taacccccaa atttgaaata taacagaaag
    12841 cattggcatc tgaccaaagc gaatacctac atagatagcg ggaataaagt ttgcaataat
    12901 tgcaagacct atagaaattc tccctatttt tatcatcttt ttatcataca taattatcac
    12961 ctcttatccg ttttaatcac gataatataa tctgtgtacc aaacatacca attaacatgg
    13021 caatacctaa ggataattct tgtaatcgtt gattcttttt aaataaagtt gtcaatacta
    13081 gcataactat agctgaaata attgctggtg taagttttgg tatttcaaat gtttttattt
    13141 gatttgaaag aaggtaagca aataatccaa ctgtagcacc tgcaagtagt tttttaaccc
    13201 aagtaggatt atacttcatg ttcatctttt caacaattcc acgcattcta tgacccaaaa
    13261 taaatacaac aaatagccaa cctaagttat ttaaagccat cccccatagt gcatacgaaa
    13321 aagcttctgc tccaaatgca gcactaccta cttctacccc agataaattg gatgtaagag
    13381 atattactcc aagctctgtt ctagctgctc cgacatcaca gagcctcatc caagtcgtag
    13441 gagcgccaat cacagctacc agtgataata atgtaataac aggagataga gaaggtccaa
    13501 tggctgtaac actagctgag cgaattgctg ctttaattcc tttcttttca ataccaagat
    13561 ttgaagcctc ttttagtgcc tctttcataa atacgattgc ttgaacaact gaaataatta
    13621 ctaaaaaact agaaacaatc caaagacctg ggctattaat catttcttga aacttcatac
    13681 tttattcctc cttaaaataa atttttcagc attttattgt gagctttctt tctgtaatct
    13741 tgttatgttt ttatattgtt ttaatttatt ttatctaaga tatagtatct ttaataggag
    13801 attatctgtc ttaaagtctc actaaaaaca ttaatactta aatattttat tcataattag
    13861 taaatatcag aaaattcaca tattattttt ttaacctgta attaacttat aaggtctttt
    13921 attattttga catttcaata taaaaatttt aatcaaaaaa ctttattatt ttaaaaaaaa
    13981 ttattatttt tataatttat attttttaag tttatttatg ttcatctcta tctaaaataa
    14041 tgacatttct aactcttaat tctcatttaa gtttcattgt tttttcttaa aagcctactt
    14101 ttagtcaaga tactgttatc tttactcatt atttaaagaa cttattttaa atctgtacat
    14161 gaagtaaata atccaaaagc attgttattc taactactca acattaaata cttactattt
    14221 tctaattact caatataaag acttactata aaactaaata cataaatggc ttctaaagtt
    14281 taatatatct acatggcttt atatatgaca ggaaaaatac tagaaaaaat atattataga
    14341 atacttgtat ataataggca aaactttcaa acttaaattt atcaaataat cataaaaaaa
    14401 gtgaaccatc tatagtaaaa ttttatctaa taagtgaaag gcaaaaaagt agcgctacga
    14461 tttgcataga agggaaggaa cttagttgga taaaactaca tttacaaaca atatacttga
    14521 atcagaacag actttgtatc gagtatctaa gtctattcta ggaaatgacc aagactgtga
    14581 agatgccgtc aacaatgcta ttttaaaagc atatgaaaag ttggattctc ttaaggaaga
    14641 acaatacttt aaaacatggc tgattagaat agtcataaat gaatgtaatt cattaagacg
    14701 caaacgatta aaatctctat catttgaaga tgtcttcaaa aacaaaaaaa ttgatgaaaa
    14761 agatgactat agcgatttgt atactgctat tcaaagtcta cctaaaaaaa ttaaaattcc
    14821 aatagtcctt tactatattg aaggttattc agtagatgaa gttaaggaaa tattagatat
    14881 acctcaagga actgtaaaaa gtagattatc aagaggaaga agattattaa aaactaaatt
    14941 agaaaacaca gaggtaatca tatgaataaa aatatattaa ataattcgtt tccagaagtt
    15001 ccagaaagtt ttcataatag tttaagcaaa acactaaata gtttaccaga aagagaggaa
    15061 aattataaaa tgattaataa taaaacacac aaattaccat ttagaaaagg cttagttgca
    15121 actttagcca ttactttgat attgagcaca acagcccttg cagtaggaca tatatatagt
    15181 ttagttggaa aatcaagtac taaggctacc tatacatcta tcccaactac agaacaagta
    15241 aaaaaagact ttaactttac ccctaatata gtttctgaat ttagtaatgg atataaattt
    15301 aaaggagcat atactgtaaa taaaaaagca tttgacaaag aaaataatgt tttaggaaaa
    15361 tcaaaagcac ttagttttga atataaaaat ggcaatgatg aaatatctct tgacacagca
    15421 aataatgtac ttgggaagag ttctgacaat ctaaaagttg tagatactta caaagatatt
    15481 aatatttctt atgaagcatt cgcacaaaag tttataccag aaggttataa aatgacagaa
    15541 caggataaaa ttgatgaaaa atcaggcaaa tacacattta gtgtggggcc aaataatgaa
    15601 tcagacaaag ttgaagttaa tgaatttaag tttttgacat ggaaacaaga tggagtttac
    15661 tattctttta cagttcaaga ctcaaattta tctatgaatg acttagttaa aatggctcat
    15721 gaagtaatag atgcaaaata aacataagta ataataagtt gtttatgagt ttattattta
    15781 tgactttaat ttttttatta gtaaacatgg ttgtatcttc catctttata ttttctttaa
    15841 atttaatata taaaatttta gatatttagc taccttcaaa agaagcatta ttaaaaagcc
    15901 cttaaaaata tttcttttat atggaatttt aagggcttta actttatttt atacaagtgt
    15961 atatggaatt ttaaagactt taactttatt ttatataagt gtaagctcca tttgaattac
    16021 ttcttctttt acaggtctat cattccatgt aaaaccacta gatattgtat ttacctctac
    16081 aaaccctaaa gatttataaa catgaatagc tctactatta cttttaaata catcaagctc
    16141 aattttttca tacccatatt cttttgataa atcaataatt tgttttaaaa gactacttcc
    16201 gatacctttt tccctatact ctttaagaat acccatgcct aaataaccaa ctttttcagt
    16261 tttaggtaaa acgtcacacc aaccaataca gttatctgat tctaaatcaa ttataaacag
    16321 ttgtggtaag tttttattta taatattttt tataaattca actgtttctt caaaaggaaa
    16381 ggcttcatcc atagctagat atttcctctc ttttgctatt ttatcaaaag tttgccaata
    16441 cgaataaata tacttttctt ctgcctttat aaattttatg ccttctatca acttaacatt
    16501 tccccttttt aatttttgat tgtagttaac ttattttata tgattctagt ttataatgct
    16561 atataaatta ctatatatta gcttaaaata tctttgtata tttctataaa attaaaacca
    16621 atatatctat ctcttgcatc tctattaaaa taatttttaa tagtataata aatatagttc
    16681 tttaatcttt tgggtatata tacttaatca ttttgaaatt tgatttaaag tataataaaa
    16741 tatataaaaa aatatataaa atggtactat aaaattttta atcttcatca ataccttttt
    16801 tgctttcatt gttaaaacta ttttttctat tagtttattt cttataaaac aaacatttag
    16861 tattaaaata tatatcacac aatagtattt gcaatttaac tttaaaaaaa taaaaatacc
    16921 caactgacct aagcaactcg attgggtata aataatctaa aaaatcattt ttgtataaac
    16981 tctttattta acttacattt ttatttacct tctactttaa ttcaacttat atttctatgc
    17041 ttcttatact ctcattcaac ttatatttct acttatctta tacttttata aattcatcac
    17101 aattatttag ttctcgctat caaagaaatt gtattaaaat actttccatt ctctacttcc
    17161 atcatttttt tgtcatttat agcatatgaa ttatcacaat ttatccaatc cttccaagct
    17221 tcatcatgac aatttaaagc ttcacatttc tctacaatca cagactccga ttcagaccaa
    17281 agtttattcc accatgtaat tgaatggaag ttcatattat cttgccaaaa tggaatgagt
    17341 tcatctggta tacaatccac gaaatcttct tttaatccag gcattgccat agcaagtatt
    17401 cccccctctt ttacaagagg tgatatatga ttgtctaaaa atccttcttt gtttccaaaa
    17461 tagtgataag aatcgatact tatcaccgca tcaaagaaac cttctgcata aggtagttca
    17521 tgtgcctctg cctgtattgg aaatatttta tcatccaatt taaactcttt aaatctttta
    17581 tagttttcag ttggttctat ccataaatct gttgcaaata cagtagcatc aaattccttt
    17641 gctaaaaata tcgaagatat acccttacca caacctaagt ctaaaatacg cattcccttt
    17701 tctagtttta tcttacttgt taattcttcc aatattttta tacaattagg tcccatcata
    17761 tttttcatga gaaagtcctt atcgtattta ttactttttg taaatatcat tctatctttc
    17821 ctttccctat cagttattat ttattaaatt ttatacgttt gattttttat tttttatctg
    17881 cttcttatat tgagcaatct cttttttatt cattctttaa cagacaattt taatgtgtaa
    17941 ttttatccac tctgtgatag taactttaat gaataatctt atctactgtg atagttattt
    18001 tagtgagtaa ttttatctac tccacagcaa tcgctttaat gagtaatctt atccactcta
    18061 tgatagttat tttagtgagt aattttatct actccacagt aatcgcttta acgagtaatc
    18121 ttatctactc tataatagtc actttaatga gtaatcataa tttttatatc attttagata
    18181 cttactctaa taagaattca ttttctaata cttaataagt tttaattact tccactttaa
    18241 cctatatact aattaactta tttaatttag tgcatatttg acactttacc ttttattata
    18301 ccatttatta tcataaatat aataattcta tttttttaaa ataaatattt ataataa
//
LOCUS       DAMJYR010000070        18234 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.070, whole genome shotgun sequence.
ACCESSION   DAMJYR010000070 DAMJYR010000000
VERSION     DAMJYR010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 18234)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18234)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18234
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.070"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(109..960)
                     /locus_tag="PC471_002354"
     CDS             complement(109..960)
                     /locus_tag="PC471_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HDF2789481.1"
                     /translation="MALVTTTSMLKKAQDEGYAVGAFNVENMEMVIAVIEAAEELNSP
                     VILQTTPSTVKYAGLDYYLANAKVAAERVNIPVAMHLDHGSSFDLAMKALRSGYTSIM
                     IDGSHESFENNISISKSVVKACSPSLIPVEAELGKVGGKEDDLDGGNSNDYTNPKEAK
                     EFVDRTGISSLAIAIGTAHGLYKGEPKIDLDRLSEIKNVVSIPLVLHGGSGIHDDIIK
                     EAIKRGIAKVNYATELRIAYSKGVKKVLDEDSEVIDPKKYGLAGLQSVKDFVKEKIKV
                     CGSVNRV"
     gene            complement(1009..2178)
                     /locus_tag="PC471_002355"
     CDS             complement(1009..2178)
                     /locus_tag="PC471_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HDF2789482.1"
                     /translation="MKMFKLEEQKLKDLGAIITTNEIKQQPELWLETYEIYKSNKEKL
                     SRFIDTISNNHGQFRVIFTGAGTSAYIGNSILPYLKNKNDIRKYIFEAIPTTDIVSNP
                     YDYLKKDIPTLLISFARSGNSPESLAALNLGNKIVDNFYHLAITCNPEGELAKMTKND
                     ENNYLLLMPSKSNDEGFAMTGSFSCMMLSAMLIFDSLEDDVEKSYINAIIEMGRNVID
                     RKDEIHELINKDFDRVVYLGSGGLGGLTQEAQLKLLELTAGKISTVYDSPMGFRHGPK
                     SFIDENTLVFEFVSNCLYTRKYDLDVLEEIKRDKIAKFTCAVSVENENNFSGTKFEFK
                     EKYNKLPDVYLAMPYILFAQTIALFVSVKVGNKPDTPSATGTVNRVVKGVTIYEY"
     gene            2649..3566
                     /locus_tag="PC471_002356"
     CDS             2649..3566
                     /locus_tag="PC471_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDF2789483.1"
                     /translation="MKNIKMGMIGLGSIAQKAYLPILTKSERFEFVGAFTPNKVKREK
                     VCSDYRIMPFDSIESLAKKCDCIFLHSSTETHYEIIKILLNLGVHVYVDKPLASTVSQ
                     GEELIELSTKKNLNLMVGFNRRFCPMYKEIKNNATEIVSINICKHGLNSLRNVRFDST
                     LIDDYIHVIDTALWLANEDVEISGEDLFLTDNKNLIFVSHKLKGKNFSINTSMHRDSG
                     TKLEQVEILSKGKIQRVKNLNVLEIEEGGNLTLKQSGAWVNILKQKGFEDISNHFIDC
                     IENNIKPAINGEECIKAQRLLEKIINSVK"
     gene            complement(3695..4624)
                     /gene="pfkB"
                     /locus_tag="PC471_002357"
     CDS             complement(3695..4624)
                     /gene="pfkB"
                     /locus_tag="PC471_002357"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HDF2789484.1"
                     /translation="MITVITFNPSIDRMYRVNNINIGEVQRVVSKNATAGGKGLNVAK
                     VCKILKENPLAMGFLGGFNGEFIKNELRKLDIQNKFTKIEQETRNCLNIIEDNKVSTE
                     FLEKGPIVEKSDLERFENDIEEVLKNTKILVASGSYCENMPIDYYEKIGNICRANNIK
                     FILDTSGEALKIALKSKPYLIKPNIDEIRHLLGMNIESREEIILSGKKLIEMGAENVC
                     ISLGKDGMVYLNEDNVYDVKVPVVKCINTVGSGDSTVAGFSVGILRGYKIENLLKFAN
                     ACGISNALNMETGFVDLGEIDKYQDLVEVKKLS"
     gene            4893..5618
                     /locus_tag="PC471_002358"
     CDS             4893..5618
                     /locus_tag="PC471_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDF2789485.1"
                     /translation="MIKRNDKRPIYDQLVEILRTKIENEMEPNDRMLSERKICDEYGV
                     SRTTVRLAMAELEHMGYIYKRHGKGTFVAALSQNSQNLMESYSFTDYMRTQGKKPGTK
                     VLSFEVVESTKYFSEKLGITPGEKMIKIMRLRLADDLPMMLERTYLPMKEFIGLTKEK
                     IEQKPLYEIFREDYGEIIKVADEEFSAGIISDKEAKLLEVPGDSPCLKLLRTTYNDDN
                     IVIEFTLSVARSDKFVYRVRHVR"
     gene            5701..6855
                     /gene="nagA"
                     /locus_tag="PC471_002359"
     CDS             5701..6855
                     /gene="nagA"
                     /locus_tag="PC471_002359"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HDF2789486.1"
                     /translation="MKSFIYADKFFMKHGIKEKGYLSIINGKFGEFQQERPNVTDNVI
                     DFSGKYIAPGLVDTHIHGLLGADVMDNTFEAINTISKGLLKYGVTSFLPTTLTDSVDT
                     LSDSVENISNSFNRVEGAKIQGIYLEGPFFTEKYKGAQNEKYFKDPDINILKLWQKKS
                     KNLIRKIAIAPERKGSIELTRYATKNNISVSLGHSSATFEQVKQVVNSGAKVFVHTYN
                     GMSPLNHREPGMVGAAMVLKDTYAELICDGHHVSPIAVKIMMDAKSSNNIALITDCMR
                     AGAMEDGKYTLGKFNVNVKNSVARLNSGSLAGSVLTMDKAVRNIVDWNISTLEDAIKM
                     ATYVPALSCNIDNVCGSIYQGMDADFIVTDNDFNIHETYINGICKYKVNK"
     gene            complement(7564..8034)
                     /locus_tag="PC471_002360"
     CDS             complement(7564..8034)
                     /locus_tag="PC471_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HDF2789487.1"
                     /translation="MYNNIKNILKENHITFEEYDHEPILSFEKAKEIREKLQYTGVES
                     KSLFIKDRSGNYYIFVTTEGKKLDPKTIKNVLGGKVSICSKEELTQKTQCLPGCVSPF
                     GYEKNISLVIDQDIFNHDKIIFSPGIPEKTFIITASGFKKVINNINNTTYKYIY"
     gene            complement(8638..9795)
                     /locus_tag="PC471_002361"
     CDS             complement(8638..9795)
                     /locus_tag="PC471_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HDF2789488.1"
                     /translation="MYLVISKKKAIFLGVILVIITAMVTSAFQLTLGNKVVISKELYE
                     DYKKYDKLLGLESIIKQDFYKKVSDTDLVNGAAKGLFLGTNDKYSGYYTKDEMENLIN
                     DSEGSYVGVGMYIGASKDGGLVVVPMKDSPAEKAGVKSGDKLVKVNGKSVSYKNSDEA
                     VRMMKGKKGKTVELTILREDKQLNFKVKTDQIIEKSIESKVIDNDLGYIEITQFISST
                     YTDFDKALKELKAKNIKGLVIDLRNNPGGMLDICKEVADELIGEGTIVYTKDNKGNTE
                     YLKSDKEKLGLPIVVLTNGESASAAEILTAAIVDNKEGISVGTTTFGKGLVQSVVRLK
                     DGTGYKLTTAQYFTPNGDYINEKGIKPTIEEKDENKQLDVATKWLREQIDK"
     gene            9949..10518
                     /locus_tag="PC471_002362"
     CDS             9949..10518
                     /locus_tag="PC471_002362"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HDF2789489.1"
                     /translation="MKKEFRKKVIESRKNKSANFVLSNSNLITEKLLQMEDIRNATNI
                     MLYLNFNNEVQTDNLIKKLLSLKKIVSSPITIKENHTLIPTQITDLKEGLRIGAYGIR
                     EPNEKSPTIDIKALDVVIVPAVAYDIHCYRLGYGGGFYDRFLENLRKDAITIGIAFDF
                     QIFDSIPKETHDAQLDYIVTETRIITPNN"
     gene            complement(10700..11116)
                     /locus_tag="PC471_002363"
     CDS             complement(10700..11116)
                     /locus_tag="PC471_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2789490.1"
                     /translation="MNVKFNIKGIIYGVIALVFVIGGFIFIPSMFVEKSKPIDYTVLQ
                     RNSIPEKILDVMDKYTNEERALAAKIDNKIYIIVTRGNNKYGIEMDKIESVVEEGKDV
                     LRVKIKYKDKEDSFPYIVVETNMSELPDRIELNATK"
     gene            complement(11342..12436)
                     /locus_tag="PC471_002364"
     CDS             complement(11342..12436)
                     /locus_tag="PC471_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2789491.1"
                     /translation="MSEKVNVKTVISFAGAYVATVIGSGFATGQEIMQFFSFYGFAGI
                     IGGIISMVLFSWMGASVMSKGKELQLKEPIKIYRVYCGKYLGIFFEWFGPLFLFGVFV
                     VMISGAGATLTEYYGLNPFVGRVGMAVVALLTVSLGLDKLSKILGGIGPIIIIFTLLV
                     GGISLAKNIGNIGEAAQVLSTVKIAKPVPNAYLSGVIYTTYNTIVVMAFLTGLGASAA
                     NKKEAICGGILGGVALMAAAIMMHLAILSDIGNLYSKAIPSLFLADKISPVIGVLFSI
                     ILILGIYTTAVPLLWSVTNRFVDDKHPKFKLITLIAAILGLIGGFLPFDKLVGILYPY
                     TGYMGVIILVCVLYRQITKTSGYQEGISDK"
     gene            complement(12859..13800)
                     /locus_tag="PC471_002365"
     CDS             complement(12859..13800)
                     /locus_tag="PC471_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HDF2789492.1"
                     /translation="MSKMATREAYGKALVKLGQINDNVVVLDADLSKSTKTHDFYKSF
                     PDRFFNMGIAEQNLIGAACGLSTAGKIPFASTFAMFATGRAFEIIRNSVCYPKLNVKI
                     CATHAGLTVGEDGASHESVEDIAIMRAIPNMTVLVPADGVETEKIIFEIAKYNGPVYV
                     RLGRSSVPVLFDEDYKFEIGKGTVLREGKDVSIIACGIMVNEALLAQEKLQEEGISAR
                     VINMSSIKPIDKDLILESAKETNAIVTVEEHSIIGGLGSAVSEVVGESCPTIVKKVGI
                     KDTFGESGTPNELLKKYELTCDDIIKTVKEVIIAKRM"
     gene            complement(13793..14620)
                     /locus_tag="PC471_002366"
     CDS             complement(13793..14620)
                     /locus_tag="PC471_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HDF2789493.1"
                     /translation="MRDHKGLNEIARIIRRDIVSMIHRAKSGHPGGSLSVVEILTALY
                     FDEMNVDSSNPKMEDRDRFVLSKGHAAPALYATLAEKGYFDKEELNGLRKIGRMLQGH
                     PDMKGTPGVEISTGSLGQGFSVACGMAMASKLDNAPWNVYTLLGDGEVQEGIVWEAAM
                     SAAHYKLDNLIAFLDNNGLQIDGDIESVMSLGSIVDKFKAFGWNVIEIDGHDFDQIFA
                     ALDIAKSTVKKPTMIVAKTIKGKGISFMENQAGWHGTAPSDEELEKALLELGGADNE"
     gene            complement(14791..15174)
                     /locus_tag="PC471_002367"
     CDS             complement(14791..15174)
                     /locus_tag="PC471_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HDF2789494.1"
                     /translation="MKGRLPKVSTNCEIKRGDLYYADLSPVVGSEQGGVRPVLIIQND
                     VGNKYSPTVIVSAITSQINKAKLPTHVEISSNEYGLNKDSVILLEQIRTIDKKRLREK
                     IGCLDENMMIKVDDGLQISLGLFTF"
     gene            complement(15143..15430)
                     /locus_tag="PC471_002368"
     CDS             complement(15143..15430)
                     /locus_tag="PC471_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="HDF2789495.1"
                     /translation="MYDKSKEKIEVTLPDSLISEVDSIVQMENSNREDFAKAAFQFYI
                     TQKKKIHIKESMKTGYREMGQINLSLAELGMTVEEVSSDDKNEGKIAQGEY"
     gene            complement(15463..16620)
                     /gene="alr"
                     /locus_tag="PC471_002369"
     CDS             complement(15463..16620)
                     /gene="alr"
                     /locus_tag="PC471_002369"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HDF2789496.1"
                     /translation="MQKITVPTWAEINLDNLRFNLNNIKNLLEEDIKICGVIKADAYG
                     HGAVEVAKLLEKEKVDYLAVARTAEGIELRQNGITLPILNLGYTPDEAFEDSIKNKIT
                     MTVYSLETAQKINEIAKSLGEKACVHVKIDSGMTRIGFQPNEKSVQEIIELNKLEYID
                     LEGMFTHFATADEVSKEYTYKQANNYKFMSDKLDEAGVKIAIKHVSNSAAIMDCPDLR
                     LNMVRAGIILYGHYPSDDVFKDRLELRPAMKLKSKIGHIKQVEPGVGISYGLKYTTTG
                     KETIATVPIGYADGFTRIQKNPKVLIKGEVFDVVGRICMDQIMVRIDKDIDIKVGDEV
                     ILFGEGEVTAERIAKDLGTINYEVLCMISRRVDRVYMENNELVQINSYLLK"
     gene            complement(16635..17225)
                     /locus_tag="PC471_002370"
     CDS             complement(16635..17225)
                     /locus_tag="PC471_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2789497.1"
                     /translation="MRKKWTIVCIMFLALVIIVIGCQKRQSTKEEVYKDFQKQISDMN
                     YYSCKAEVEVVGNKSPHNYVLIHTYKKTDNYKLEVISPKHLKGKSIEYQDDKILVKNP
                     KISDVVELPNTGKNNQYLFVGDFIKNYLQNEEMKVKLSKGHLVLETFIPGDNKYFNKQ
                     VLYVNADTKNPEKMEVLDKEGVPRFTVKYKDFEYRN"
     gene            complement(17246..17707)
                     /locus_tag="PC471_002371"
     CDS             complement(17246..17707)
                     /locus_tag="PC471_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HDF2789498.1"
                     /translation="MMDKTAKEIMTTDVIVAKQDDSIADVANMLIAEKIGGLPVVDSE
                     NRVVGIISETDILKKEKYIEAPLYINLLQGLIFLDDLKKVEKDIKQVAAYKVGELMSK
                     DIIKVHEDDKFDDVANVMIKKSINRVPVVDDDNKLKGIICRYDIIKALYNE"
     gene            complement(17722..18102)
                     /locus_tag="PC471_002372"
     CDS             complement(17722..18102)
                     /locus_tag="PC471_002372"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HDF2789499.1"
                     /translation="MNIFDIGVDIIEIDRIRKAVDKNNRFLEKIFTDREIEYFNSKNF
                     KAESIAGNFAAKEAISKSIGTGIRLFNFKDIEVLRNEMGKPIVKTYNNLAKMCIDYNV
                     LEIKVSISHSKDYAIANAITIIKD"
BASE COUNT         6111 a         3035 c         2089 g         6999 t
ORIGIN      
        1 tttcatcaat taaatgaaaa aaatacacca tttacatata attaatataa atttttcata
       61 taataaatat cctaaactta tctaaaataa atttaggata ttttattttt atactctatt
      121 tacagaccca cacactttaa tcttttcttt cacaaaatcc tttactgatt gaagccctgc
      181 taatccatac tttttagggt ctataacctc agaatcttca tctaaaactt ttttaacccc
      241 tttactatat gcaattctta attcagttgc atagttaact tttgcaatac ctctttttat
      301 agcttctttt ataatatcat catgtattcc tgaacctcca tgcaatacta aaggtattga
      361 aactacattt ttaatttctg ataacctatc taaatctatt ttaggttcac ccttatacaa
      421 tccatgtgca gtacctatag caatagccaa tgaagatatt cctgttctat cgacaaactc
      481 ttttgcctct tttggatttg tataatcatt actattcccg ccatctaaat catcttcttt
      541 tccaccaact ttcccaagtt ctgcttccac tggtataaga gaaggactac atgcttttac
      601 tactgattta gatatagata tattattttc aaagctttca tgagaaccat ctatcattat
      661 tgatgtatat ccacttctta aagctttcat tgcaaggtca aaacttgaac catggtccag
      721 atgcattgct acaggtatat ttactctttc tgcagcaacc tttgcatttg ctagataata
      781 atcaagacct gcatatttta cagtagatgg agtagtttgt aatattactg gagaatttaa
      841 ctcctcagca gcttctataa cagcaattac catttccata ttttctacat taaatgctcc
      901 tactgcatac ccttcatctt gtgccttttt aagcattgat gttgttgtta ctaatgccac
      961 attgataccc tcccaaatta tttagttata atattttaat cttatctttt aatattcata
     1021 tattgttaca ccttttacta ctcgatttac tgttcctgta gctgatggag tatctggctt
     1081 atttccaacc tttactgata caaacaatgc tatagtttgt gcaaacaata tgtatggcat
     1141 tgcaagatac acatctggta atttattata cttttcttta aactcaaact ttgttcctga
     1201 gaagttattt tcattttcta ctgacactgc acaagtgaat ttagcaattt tatccctttt
     1261 tatctcctct aaaacatcta aatcatattt tcttgtatat aaacagttag atacaaattc
     1321 aaatacaagt gtattttcat caataaatga ttttggtccg tgtctaaacc ccataggaga
     1381 atcatacact gtagatattt ttcctgctgt aagttctaaa agttttagtt gtgcttcttg
     1441 tgtcaaacca cctaatcctc ctgaacctaa atatacaact ctatcaaaat ctttatttat
     1501 taactcatgt atttcatctt ttctatctat aacatttctt cccatttcaa ttattgcatt
     1561 tatatatgac ttttctacat catcttctaa actatcgaat attaacatag cagataacat
     1621 catacacgaa aaacttcctg tcatggcaaa gccttcatca tttgattttg atggcattaa
     1681 taacaagtaa ttattttcgt cgtttttagt catcttagcc agttctcctt ctggattaca
     1741 agttatagct aaatgataaa aattatctac aatcttattc cctaaattta aagctgctaa
     1801 actttcagga ctatttccac ttcttgcaaa tgaaattaat aatgttggta tatctttttt
     1861 taagtaatca tatgggtttg aaactatatc cgtagtaggt attgcttcaa atatgtattt
     1921 tcttatgtca tttttatttt taaggtaagg aagtattgaa tttcctatgt aagctgaagt
     1981 tccagcacct gtaaatataa ctctaaactg cccatgatta ttacttattg tatctatgaa
     2041 tctacttaat ttttctttgt tagatttata tatttcataa gtttctaacc ataattcagg
     2101 ctgttgtttt atttcattag ttgttattat tgcacctaaa tcttttagtt tttgttcttc
     2161 taatttaaac attttcattc tcctttcata cttgtaacgt ttacatttgg taatgaggtt
     2221 ataaccactt taaactataa aaaaatatac tcttttataa ttttttgctt agatttttta
     2281 gtactactta aactcactat aaaatataca ctatagattt taattaattt tatagtttat
     2341 ctttaaaaat ttaataaaat catactctat atactttagt atttttattt tacgataaaa
     2401 gcttgttcag accaaagtgg taattaccac taatccaaat ataaattaat ttgaattttt
     2461 tgtcaataca taaaatattt atatcaatca attttatgga tatcctacaa aattacttta
     2521 tcaatatttc ttatttcgct aatttactta tataagcttt aaatttattt tttgatacaa
     2581 agtttacaaa tttatgtgtc tgaattataa tcaattctgt aaatataaaa accttaggag
     2641 aaagtattat gaaaaatata aaaatgggaa tgataggtct tggctccata gctcaaaagg
     2701 catatttacc tatattaact aagtctgaga gatttgagtt tgtaggagct tttactccaa
     2761 ataaagttaa aagggaaaaa gtctgtagtg attatagaat catgccattc gattctattg
     2821 aatccctagc taaaaaatgt gattgtattt ttctacatag ctctacagaa acacactacg
     2881 aaattataaa aatattatta aatttaggtg ttcatgtata tgtagacaaa cctcttgctt
     2941 caacagtaag tcaaggagaa gaacttatcg agttaagtac gaaaaaaaac ttaaatttaa
     3001 tggttggatt caaccgtaga ttctgtccta tgtacaaaga aattaaaaat aatgctacag
     3061 aaattgtatc tatcaatata tgtaaacatg gattaaattc cttaagaaat gttagattcg
     3121 acagtactct tatagatgac tatatacatg ttattgacac tgctttatgg ttagcaaatg
     3181 aagatgttga aattagtgga gaggatttat ttttaacaga caataagaat cttatatttg
     3241 taagtcataa acttaaaggt aaaaatttct ctataaacac ctctatgcat agagattctg
     3301 gaacaaagtt agaacaagtt gaaattcttt caaagggtaa gattcaaaga gttaagaatc
     3361 ttaatgtact ggaaatagaa gaaggtggaa acctaacttt aaagcaaagt ggtgcatggg
     3421 taaatatatt aaaacaaaag ggttttgagg atatatcaaa tcattttatt gattgtatag
     3481 aaaataatat aaaaccagct attaatggag aagagtgtat caaagctcaa agattactag
     3541 agaaaattat aaattcagta aaatagatat ttattataaa aataggttaa aaatgaattt
     3601 ttctaaccta tttttatata agactatatc ttttatatta aaattaaaat ctattacaac
     3661 aacattaaat ttattatatt cccattaaat gtatttaact taactttttt acttcaacta
     3721 aatcttgata tttatcaatt tctcccaaat ctacaaatcc agtttccata tttaaagcat
     3781 tacttatacc acaagcattt gcaaatttaa gtaaattttc tattttataa ccccttaaaa
     3841 tacctacact aaatccagcc actgtgctat ctccactacc tacagtattt atacacttta
     3901 caactggtac ttttacatca tatacattat cttcgtttaa atacaccata ccatcttttc
     3961 ctaaagatat acatacattc tctgcaccca tttcaataag tttttttcct gacaagataa
     4021 tttcctctct tgattctata ttcattccta ataaatgtct tatttcatct atatttggtt
     4081 ttattaaata tggttttgat tttaaagcta tttttaatgc ttctcctgat gtatctaata
     4141 taaactttat attatttgct ctacatatat ttcctatctt ttcataatag tctatcggca
     4201 tattctcaca ataactacca gaagctacca gtattttagt attttttagt acttcctcta
     4261 tatcattttc aaatctctct aaatcgcttt tttctactat tggtcctttt tctaaaaatt
     4321 ctgtactaac tttattgtcc tcaattatgt ttaagcaatt tctagtttcc tgctctattt
     4381 ttgtaaattt attttgtata tctagttttc taagttcatt ttttataaat tctccattaa
     4441 atccgcctag aaatcccata gctaaaggat tttcttttaa aattttacat accttagcta
     4501 cgtttaatcc tttacctcca gcagttgcat tcttacttac taccctttgc acctcaccta
     4561 tgtttatatt attcactcta tacattctat ctatagatgg gttgaatgtt attactgtaa
     4621 tcatactaga ttctcctcat tattaatttt tacttttcta aacaataaat taatctgtaa
     4681 aataaaaaat acctcatcta taatataccc aaagtggtta ttaccaattt gaatataaat
     4741 tattttactt gttttgtcaa tgtaccataa ttttataata aattaataac cttacttatt
     4801 gaattttgat taaactttat tcattgacat aaataattct atattctata atcaaattgt
     4861 ggtaataacc acttagaaag gactatattt ttatgataaa aagaaatgat aaaagaccaa
     4921 tatatgacca gcttgttgag atattaagaa ctaagattga aaatgagatg gaaccaaacg
     4981 atagaatgct ttccgaaaga aaaatttgtg atgaatatgg agtaagtaga accacagtta
     5041 gactagctat ggctgagttg gaacatatgg ggtatatata caaaagacat ggaaaaggaa
     5101 cttttgttgc tgcactaagt caaaattctc aaaacttaat ggaaagttat agttttacag
     5161 attatatgag aacacaagga aaaaagccag gtacaaaggt attatcattt gaggtagtag
     5221 aaagtacaaa gtatttctct gaaaaattag gtataactcc tggtgaaaaa atgataaaaa
     5281 taatgcgttt aagacttgct gatgaccttc ctatgatgct tgaaagaaca tatcttccta
     5341 tgaaagaatt tataggattg acaaaagaaa aaatcgaaca aaaaccttta tatgaaatat
     5401 ttagagaaga ttatggcgag attattaaag ttgctgatga agaattttca gctggaataa
     5461 tctctgataa ggaagctaag ttattggaag taccaggaga ttcaccttgt ctgaaattac
     5521 ttcgtaccac atataatgat gacaatatag ttattgaatt tactttgagt gttgctagaa
     5581 gcgataaatt tgtatataga gtaagacatg taagatagta agttaaataa ctttcaagaa
     5641 aatagaatga tattataaac gtcaatgcca caatgatagt ataattataa ggaggttatt
     5701 ttgaaaagtt ttatttatgc agataaattt tttatgaaac atggaatcaa agaaaaaggt
     5761 taccttagta taattaatgg caaattcggt gaatttcaac aagaaagacc taatgttact
     5821 gataatgtta tagatttttc aggtaaatat attgctcctg gcttagttga tactcacatt
     5881 cacggtcttt taggtgcaga tgtcatggac aatacatttg aagcaataaa cacaatttca
     5941 aaaggcttat taaaatatgg agtaacatct ttcctaccta caactttaac tgattctgta
     6001 gatacactaa gtgattctgt agaaaatata agcaatagct tcaatcgagt tgagggtgca
     6061 aaaatacaag gtatttattt agaaggtcca ttttttacag aaaaatataa gggcgctcaa
     6121 aatgaaaaat attttaaaga ccctgacatc aatatattaa aattatggca aaaaaaatcg
     6181 aaaaatttaa taagaaaaat agcaattgct ccagaacgta aaggttcaat agaattaaca
     6241 agatatgcaa ctaaaaataa tatatcagtt tctcttggtc atagttcagc tacatttgag
     6301 caagttaaac aagtcgttaa ttcaggtgct aaagtgtttg tacatacata taatgggatg
     6361 agtcctttaa atcatagaga acctggaatg gttggggctg ctatggtact aaaagatact
     6421 tatgctgaat taatttgtga tggtcatcat gtaagcccaa tagctgttaa aataatgatg
     6481 gatgcgaagt ctagtaataa catagcttta attacagatt gtatgagagc tggtgctatg
     6541 gaggatggaa aatatactct tggtaagttt aatgtaaatg taaaaaatag tgttgcaaga
     6601 ttaaatagtg ggagtttagc tggaagtgta ttaactatgg acaaggctgt gagaaatata
     6661 gttgattgga acatatcaac tttagaagat gcaataaaaa tggctacata tgtccctgct
     6721 ttaagttgta atattgataa tgtctgtggc tctatatatc aaggtatgga tgcagacttt
     6781 atagtaacag ataatgactt taatatacac gaaacttata taaatggtat ctgtaagtac
     6841 aaagttaata aataggttca atataaggtt atggaggctg tctaaaaaga gtctggaaat
     6901 atttaaagtt atttctccaa aatctgattt aacactttta tttccatatc gttaataaac
     6961 tattgcgata gaggttattt taaacaaata tatattataa gtatttttgt gataatattt
     7021 tcgataatat gtacatttaa agtaactttt ctattattat ttgtaagttt ttatcttgag
     7081 caaacatata aatttttaat aaagcatgag ataaacccta tatttcaatt actaatgatg
     7141 agattttgat tgataacaga tatattttca aatgtaaaaa tactaaaaat catatcaatt
     7201 ttaaagatat tgtaggtgta aataaacaag ataatactat agaaattttt acaaatgaaa
     7261 gcacttattg tatatatcta aaagttttaa gcattgagag cgaagaatat ctaatagata
     7321 gactaaaatc attttgttga ttttatatat tttatgttaa atataaagca gagtagttaa
     7381 aactaccctg cttttgtaat taaaatttaa aaataataac tcataataca ttgaatttga
     7441 gcttaacagt aactattgta ataatagtat caatcttttg tgttgaatat aatttattta
     7501 cactatagtt aagcatgatt tctaatgtta ttttctctaa tttacaatta tgattaaaat
     7561 tcattaatat atatatttat aagttgtatt gtttatattg tttattactt ttttaaatcc
     7621 actagctgta ataataaatg tcttttctgg tatccctggt gaaaagatta ttttatcatg
     7681 gttaaatata tcttgatcta ttacaagaga gatatttttc tcatatccaa aaggagatac
     7741 acatccaggc aaacattgtg ttttttgtgt taattcttct tttgaacata tagatacttt
     7801 tccacctaaa acatttttta tagtttttgg atctaatttt tttccttctg tggttacaaa
     7861 tatatagtaa tttccagatc tatcttttat aaataaactt ttactttcta ctccagtata
     7921 ttgtaatttt tctcttattt ctttagcttt ttcaaatgat aaaataggtt catggtcata
     7981 ttcttcaaac gttatatgat tttcttttaa aatatttttg atattgttgt acatatattt
     8041 tcctttctat atttaaatta atttatatat aatattcaat tataaagtga ttttataatt
     8101 tcttcaaact atcataaaaa acattcacat gttttaggaa taaattatat aaaataggtt
     8161 ataagataat gaacatttat attataacct atttttatat cgataagtat gaaattgttt
     8221 aaccaagtca aaatgatata taaatttata tttaaatgtg tttagagttt aatttatttt
     8281 caatgtttaa aaaagttatt ctatgcatct aatttaatta gtaaactgag gttctagtta
     8341 gataaattat attataatga atatatagta tgcagtttac gatattttaa aagtaaatga
     8401 tataatattt ggaacaactg ggttgcaatg tgtttttaaa aaatatcaaa ataaggctat
     8461 gatttttaaa accatagccc tgtctgtaaa aatttaatta tcacttaaaa aataaatttt
     8521 tatttaaaga atacacataa atatttccag actccctcat gaactaaaaa gttcattttg
     8581 agacatctct tttataatgt tctttatttt atagtataag tttataaaaa ttaaatccta
     8641 tttatcaatc tgttctctaa gccacttagt tgctacatct aactgtttat tttcatcttt
     8701 ttcttcaata gtaggcttta tacctttttc attgatataa tctccattag gtgtaaaata
     8761 ttgagcagta gttaacttgt atcctgttcc atccttcaat cttacaactg attgtaccaa
     8821 ccctttacca aaagttgtag tccctacaga aataccttct ttattatcaa cgattgcagc
     8881 tgttaatatt tcagcagcag aagcactttc tccattagtt aacactacta ttggaagtcc
     8941 caatttttct ttatcagatt tcaagtactc agtatttccc ttattatctt ttgtatatac
     9001 tatagttcct tcaccaatta actcatcagc aacttcttta catatatcta gcattccacc
     9061 aggattattt ctaaggtcta ttactaatcc cttgatattt tttgctttta attcttttaa
     9121 agctttatca aagtcagtat atgtacttga tataaattgg gtaatttcta tatatcctaa
     9181 atcattatca attactttac tttctatact cttctcaatt atctggtctg tttttacttt
     9241 aaagttcaat tgtttatctt ctcttaatat agtcagttca actgttttac cttttttacc
     9301 cttcatcata cgaacagctt catctgaatt tttatatgag acagattttc cattgacttt
     9361 tactaattta tcccctgatt ttacacctgc tttctctgct ggagaatctt tcatgggaac
     9421 tactactaag ccaccatctt ttgatgcccc tatatacata cctacaccaa cataagaacc
     9481 ttcactatca ttaattagat tctccatttc atctttagta taataaccag aatacttatc
     9541 atttgttcct agaaatagac cttttgctgc tccatttacc aaatcagtat ctgatacttt
     9601 tttataaaaa tcttgtttta taatagattc tagtcctaac aacttatcat attttttata
     9661 gtcttcatag agttctttcg atattacaac tttattcccc aatgtcaact gaaatgctga
     9721 tgtaaccata gctgttataa ttactaaaat cacccctaaa aaaatagctt tcttctttga
     9781 aatcacaaga tacatctcct tttacataaa ctattattat tatatgacta gctgttattt
     9841 ttattacatt tacccaaata tattgcttta ttacaataaa ttatactaaa atttatatta
     9901 aatacaattg ctgtacacat tataacatat taagggagga attattttat gaaaaaagaa
     9961 tttagaaaaa aagttattga atcaagaaaa aataagagtg caaacttcgt acttagtaat
    10021 tcaaatttaa tcactgaaaa attacttcaa atggaagata taagaaatgc aactaatata
    10081 atgctttatc taaattttaa caatgaagtt caaacagata atttaatcaa aaaacttttg
    10141 tctctaaaga aaatagtatc aagtcccatc acaataaaag aaaatcatac attaatccct
    10201 actcaaataa cagatttaaa agagggattg agaattggag catatggaat aagagaacct
    10261 aacgaaaaat cacctacaat tgatattaag gccttagatg ttgtaattgt accagctgta
    10321 gcatatgata tacattgcta tagattagga tatggtggag gattttatga tagattttta
    10381 gaaaacttaa gaaaagatgc tataactatc ggcatagcat ttgacttcca gatattcgat
    10441 tctattccaa aagaaactca tgatgcacaa ttagactaca tagttactga aactagaatt
    10501 atcactccaa ataattagaa tattaagtat ttccagaccc catttaatat agtaataaat
    10561 actactgtta atatcttact ttataagcat tttaagtcat ggcttttaca acaataagaa
    10621 ccatatacaa tttacttaca ttaataaaaa ccatgacttt attgtacaat atgcagttat
    10681 ttaattataa aataccttct tatttcgtcg catttaattc tattctatct ggaagttcac
    10741 tcatatttgt ttcaacaact atgtatggaa aagaatcttc cttatcttta tactttatct
    10801 tgactctcaa aacatccttt ccttcctcaa ccacactttc tattttgtcc atttctatac
    10861 catatttatt atttcctcta gtcactataa tgtatatttt gttatcgatt ttagccgcta
    10921 atgctctttc ttcatttgta tacttatcca tcacatctaa tatcttttct ggtattgaat
    10981 ttctttgtaa tactgtataa tctatgggtt tagatttctc tacaaacata cttggtatga
    11041 atataaatcc ccctataacg aatacaagag ctataactcc ataaattata cctttaatat
    11101 taaactttac attcaatctt atcactcctc ccatatattc taaatatatg atttataaac
    11161 ttatcttaga ctctaaatta ttgattagta tatatttata cataattata aatatatgta
    11221 atgtaactaa aaaacatgtt ttttctattt cataataaac gagctaaaaa ataaaaagag
    11281 ctatatgaaa acattttcat tgttttcata tagctcttga gtttacaata ttaaaaaata
    11341 cttatttgtc actgattcct tcttgatatc cagatgtttt tgtaatctgt ctataaagaa
    11401 cacaaaccag tattatgacg cccatatatc cagtatatgg atataaaata ccaaccagct
    11461 tatcaaatgg taggaagcca cctataagac ctaatatagc tgctattaaa gttattaatt
    11521 taaacttagg atgtttatca tctacaaatc tattagttac tgaccataat aaaggtacag
    11581 ctgttgtata tatacctaat ataagaatta ttgagaataa aactccaatt actggtgaaa
    11641 tcttatctgc taagaataat gatggtattg cttttgaata taagtttcct atatcagaaa
    11701 gaatagctaa gtgcatcatt atagctgcag ccataagtgc tactccacct aatataccac
    11761 cacaaatagc ttcttttttg ttagcagcac ttgctcctaa tccagttaag aatgccataa
    11821 caacaattgt gttataagta gtatatatta caccagatag ataggcattt ggaactggtt
    11881 tagctatctt tacagtgcta agaacttgtg cagcttctcc tatattacct atgtttttag
    11941 ctaaacttat accaccaact aaaagtgtaa agattattat tataggccct attccaccta
    12001 gtatctttga taatttatca agacctagtg aaacagttaa taatgctact actgccatac
    12061 ctactctacc tacaaatgga tttaatccat agtattcagt taaagttgct cctgctcctg
    12121 aaatcattac tacgaaaaca ccaaataaga ataaaggtcc aaaccattca aagaatattc
    12181 ctaaatactt accacaataa acacgataaa ttttgatagg ttctttaagt tgaagctctt
    12241 taccttttga cataactgaa gcacccatcc atgagaataa taccattgaa attatcccac
    12301 caataatacc tgcaaaacca taaaaagaga agaattgcat tatttcctgt cctgttgcaa
    12361 atccagaacc gattactgtt gctacatacg cacctgcgaa actgatgact gttttaacat
    12421 ttactttttc tgacatatct cagttacctc cccatgtgaa attttttctt ctgattaaac
    12481 aattcgataa actacctaca atttccttta acataaaata catgataact gttttatagc
    12541 tttttagtat aataacttaa aacttataat ctgattttat accctactcc tcctctcata
    12601 atttttttag attcttttat ctaagtagtc attatatata aaattagttt ctctataaat
    12661 tatatattag tcatattttt ctgaatagtc aatatatatt tgaatatttt ctctattttt
    12721 gtaaaaattt gaatatttta ttacattttt gatatattga tatcatataa aaaaaagaga
    12781 gatataaatt aatataacac tttatacctc tcttttaaat atattatatt attaacaaac
    12841 atgaataatt tatatctctt acattctttt tgcaataata acttctttaa cagtctttat
    12901 tatgtcatca caagttaatt catatttttt taacaattca tttggagtac cagattctcc
    12961 aaatgtatct ttaattccaa ctttctttac aatggttgga caagactctc ctactacctc
    13021 acttacagca gaacctaatc ccccaattat gctatgttct tctacagtta caatagcatt
    13081 cgtttctttt gctgattcta atattaaatc tttgtctata ggttttatcg aggacatgtt
    13141 tataactcta gcacttatac cttcttcttg caacttttct tgtgctaata gagcttcatt
    13201 taccataatt ccacaagcta taattgatac atcctttcct tctcttaaga ctgtaccttt
    13261 tcctatttca aatttataat cttcatcaaa taaaactggt actgaacttc tccctaatct
    13321 aacataaaca ggtccattat acttagctat ttcaaaaata attttttctg tttcaactcc
    13381 atcagctgga acaagaactg tcatatttgg tatagctctc attatagcaa tatcttccac
    13441 actttcatga gatgctccat cctctccaac agtaagacct gcatgagtag cacatatttt
    13501 tacatttagc tttggataac aaactgaatt tcttataatc tcaaaagccc ttcccgtagc
    13561 aaacatagca aaagtacttg caaaaggtat tttaccagca gttgataatc cacaagcagc
    13621 gcctattaaa ttttgttcag ctatacccat attaaaaaat ctatctggaa atgacttgta
    13681 aaaatcatgt gtttttgtag actttgataa atctgcatct aatactacaa cattgtcatt
    13741 tatttgacct aattttacta atgctttacc atatgcttct ctcgtcgcca ttttactcat
    13801 tgtcagcacc tcctaattca agcaaagctt tttctagctc ctcatcactt ggagctgtcc
    13861 catgccatcc tgcttgattt tccataaaag atattccttt gccttttata gtttttgcaa
    13921 ctatcattgt aggtttttta actgttgatt tagctatatc aagagccgca aatatttggt
    13981 caaaatcatg tccatctatc tcaattacat tccaaccaaa agctttaaat ttatcaacta
    14041 ttgaacctaa actcattacg ctttctatat ctccatctat ttgaagtcca ttattatcta
    14101 aaaaagcaat taaattatca agtttataat gtgcagcact catagcagct tcccaaacaa
    14161 ttccttcttg aacctcacca tcaccaagca aagtatacac attccacgga gcattatcca
    14221 actttgatgc catagccata ccacatgcaa ctgaaaaacc ttgacctaaa gaaccagtag
    14281 atatttccac acctggcgtg cctttcatgt ctggatgacc ttgtaacatc cttcctatct
    14341 tccttaatcc attcagttct tccttatcaa aatagccttt ttcagccaat gtagcatata
    14401 atgctggtgc agcatgtcct tttgataata caaatctatc tctatcctcc attttaggat
    14461 ttgaagaatc cacattcatt tcatcaaagt ataacgcagt aagtatttca actactgata
    14521 atgaccctcc tggatgtcca gatttggctc tatgtatcat tgaaactata tctcttctta
    14581 tgatacgcgc aatctcattt aatcctttat ggtctctcat gcaattacct cccttgagtt
    14641 cgtaatttta tatcaattcc atattattat atcactttta tgctattttt ttgcgttatt
    14701 tttaatttta ttaacatagt acattaaaat taaaccttaa gagatattta aaagaaaaaa
    14761 gggattgtct aataaaagac aaatccctct ttaaaaagta aacaatccta aacttatttg
    14821 aagtccatca tctactttta tcatcatatt ttcatctaga caaccaattt tttccctcag
    14881 tctcttttta tcgatagttc taatttgctc taaaagaatt actgagtctt tattaagccc
    14941 atattcattg gaacttatct ctacatgagt aggtaatttg gctttattaa tctgagatgt
    15001 tatagctgat actataactg taggactata tttattacct acatcatttt gtattatcag
    15061 aacaggtcta acgcctccct gctctgaacc aacaactgga cttaaatcag cgtaatataa
    15121 atcaccacgc tttatttcac aattagtact caccttgggc aatcttccct tcatttttat
    15181 catcagaaga tacttcttca actgtcatac ctaattcagc tagtgacaga ttaatttgac
    15241 ccatttctct ataacccgtt ttcatagatt ctttaatatg aattttcttt ttctgagtta
    15301 tatagaactg aaaagcagct tttgcaaaat cttctctgtt actgttctcc atctgaacta
    15361 tactatcaac ttcagaaatc agagagtcag gtaaagtaac ttctattttt tctttactct
    15421 tatcgtacac aaaatcgcct cccagattat aatatcatgg ttttatttta gcaaataact
    15481 gtttatttgt acaagctcat tattttccat ataaacacgg tcaactcttc ttgatatcat
    15541 acataacact tcatagttta tagttcctaa gtctttagct atacgctcag ctgtaacttc
    15601 gccttctcca aatagtataa cctcatctcc aacttttatg tctatatctt tgtcaattct
    15661 aaccattatt tgatccatac atattctacc aactacatca aacacttctc ccttaataag
    15721 aacctttgga tttttttgga ttctagtaaa tccatctgcg tatcctattg gaactgtagc
    15781 tattgtttct ttacctgtag ttgtgtattt tagtccataa cttattccta cacctggttc
    15841 aacttgtttt atatgtccga tttttgattt taatttcatg gctggtctta attctaatct
    15901 atctttaaat acatcatcag atggataatg accatataat attattcctg ctcttaccat
    15961 atttaatctt aaatcagggc aatccataat agctgcactg tttgatacat gttttatagc
    16021 tatttttaca ccagcctcat ctaatttatc agacataaat ttataattat tagcttgttt
    16081 ataagtgtac tctttactta cttcatcagc tgtagcaaaa tgagtaaaca taccttctaa
    16141 atctatatat tctaatttat ttaattctat tatttcctgt actgacttct cattaggttg
    16201 gaaacctatt ctagtcatcc ctgagtctat tttaacatga acacaggctt tttctcctaa
    16261 agattttgca atttcattta tcttttgtgc tgtttctaaa gaataaactg tcatagttat
    16321 tttatttttt atagaatctt caaaagcttc gtctggagta tatcccaagt tcaaaatagg
    16381 aagtgttatg ccattttgtc taagttcaat tccttcagca gttcttgcta ctgctaagta
    16441 atctactttt tctttttcta gcaattttgc aacttctact gcaccatgtc catatgcatc
    16501 agcttttatt actccacaaa tcttaatatc ttcttctaat aaatttttaa tattatttaa
    16561 gttaaatctt aagttatcta gatttatctc tgcccatgta ggcactgtta ttttttgcat
    16621 gtctttagtc ccccttagtt tctgtattca aaatctttgt attttactgt aaatcttggc
    16681 actccctctt tatctaacac ttccattttt tcaggatttt ttgtgtcagc atttacatat
    16741 aatacttgct tgttaaaata tttattatcc ccaggaataa aagtctctaa aaccaaatgt
    16801 cctttggata actttacttt catctcctca ttttgaagat agttttttat aaagtctcct
    16861 acaaaaagat attgattatt ttttcctgta ttaggtaact ccactacatc actaatcttt
    16921 ggatttttaa ctaatatttt atcatcttga tattctatac ttttcccttt taaatgtttt
    16981 ggggaaatta cttctaattt atagttatcg gtttttttat aagtatgaat taaaacataa
    17041 ttgtgtgggc ttttatttcc aactacttct acttcagctt tacaactgta ataattcata
    17101 tcagaaatct gcttttgaaa atctttgtat acttcttctt ttgtggactg tcgtttttgg
    17161 catcctatta caattataac cagtgccaag aacataatac atactatggt ccactttttt
    17221 ctcacatcac acctcctact tcagtttatt cattgtataa agcttttatt atatcatatc
    17281 tacaaattat acctttaagt ttgttgtcat catctactac aggaactctg tttattgatt
    17341 ttttaatcat tacattagct acatcatcaa acttatcatc ttcatgaacc tttattatat
    17401 ctttagacat aagttctcca accttataag cagctacttg ttttatatct ttttcaactt
    17461 tttttaagtc atctaagaaa atcaatcctt gtaatagatt tatatagaga ggtgcttcta
    17521 tatatttttc ttttttaaga atatctgttt cagaaattat tccaacaact ctattctctg
    17581 aatctacaac tggcagacct cctatttttt ctgcaattag catattagct acatctgcta
    17641 tactatcatc ttgttttgct actattacat ctgttgtcat tatttctttt gctgttttat
    17701 ccatcataat gtaatcactc cttagtcctt aattatggtt atcgcatttg ctatagcata
    17761 atcttttgaa tgtgatattg aaacttttat ttctaaaaca ttataatcta tgcacatttt
    17821 tgctagatta ttataagttt ttactattgg ttttcccatt tcatttctga gaacttctat
    17881 atctttaaaa ttaaacaatc taattcctgt acctatagat ttacttatag cttccttggc
    17941 tgcgaaatta cctgctatac tttctgcttt aaaattctta gaattaaaat actctatttc
    18001 tctatctgta aatatcttct ccaaaaacct attatttttg tctacagcct ttcttattct
    18061 atcaatttca attatatcta cgccaatatc aaatatattc attgttcctc cagaaaattt
    18121 ataatacttc tttcaataat taataatgaa tttataaatt tactatcgat aatacacatt
    18181 attatttagt ttatgttact tatcttaatt taataatcat tataataaaa ctgt
//
LOCUS       DAMJYR010000071        18192 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.071, whole genome shotgun sequence.
ACCESSION   DAMJYR010000071 DAMJYR010000000
VERSION     DAMJYR010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 18192)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18192
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.071"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(94..780)
                     /locus_tag="PC471_002373"
     CDS             complement(94..780)
                     /locus_tag="PC471_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HDF2789500.1"
                     /translation="MFGVIANSLAIAGGCIVGLIIKGGLPQRVSDTIMNGIALCVLYI
                     GISGALEGKNTLVTIISVAVGALIGELIDIDKWVNKLGAFLQSKFSKGNKKDSIAEGF
                     ISSSLLFCIGAMAVVGSLESGLTGSHDTLFVKSVIDGIASIIFTASLGIGVMFSAISV
                     FLYEGIICVGASFLNGFLSDPVVTEMTAAGSLLIIGLGLNVLKLTNIKVANLLPAIFI
                     PILFGVFGII"
     gene            complement(829..1362)
                     /locus_tag="PC471_002374"
     CDS             complement(829..1362)
                     /locus_tag="PC471_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HDF2789501.1"
                     /translation="MSDLNNKKEQDENESSMNETKIYDILDKLNVEYDVVEHEAVYTA
                     EQLVILDGITKGCQCKNLFLRNAKGNKYYLIVVKGEKQVDLNSLKDKIGSSRLSFASP
                     ERLYNVLKLLPGSVNPFSLINDTERKVELYIDKDVIKEDYLNFHPNINTKTVNISRKG
                     FEEFLEYLKYPIQSIDV"
     gene            complement(1406..2038)
                     /locus_tag="PC471_002375"
     CDS             complement(1406..2038)
                     /locus_tag="PC471_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HDF2789502.1"
                     /translation="MKVFDLTHVTHNDMPVYAEPNRPDIKKVAIIEENGYQETLISVF
                     SHNGTHMDSPRHMYTKGETLDKLDIENFVGKAYVLELEKGNENIELEYLKKYEDEIKN
                     SDFIIFKSGWSKFWDKKQYYVGYPTLTKEAANYIANTNIKGIGIDMLSVDRYDTSVFE
                     VHHILFEKGKIIIENLTNLENVPEKFLFIAAPFKYNDADGAPVRAIAIVE"
     gene            complement(2128..3207)
                     /locus_tag="PC471_002376"
     CDS             complement(2128..3207)
                     /locus_tag="PC471_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIR2 family protein"
                     /protein_id="HDF2789503.1"
                     /translation="MKIAIFLGAGASAAENLPIQNELFSEYFKKLNPTNYNTKMNREL
                     YIFFKQMFNIDIVKDNIDNANFPTFEEVLGLLDLAEQRKEAFRNFGLENLNNRSDSIR
                     FLRQYLILLMAEAIHNTPRTDNKYHKLLVENLLENDLLLDTTFISANYDIHIDNTVTG
                     LRDKNDLPIMLDYGVEFTNFKEELNWRKPKEPLIKLYKIHGSLNWLYCPICNSVTLTP
                     HEGGVMKLIENSSETKCLECGELTEPIIVPPTYFKNMSNIFLSNVWNETEKTLRDTDL
                     LIFCGYSFPEADMHIKYMLKRVQTNRKKPPLKIMVFNNHSQKQRITLKKEEGRYKRFL
                     GEDVIFTDNSFQDFSVNPLRFIKNI"
     gene            complement(3340..4686)
                     /gene="pepV"
                     /locus_tag="PC471_002377"
     CDS             complement(3340..4686)
                     /gene="pepV"
                     /locus_tag="PC471_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HDF2789504.1"
                     /translation="MKEQIKEKVNSLQDEMISSIQESVKIPSVISEATENCPFGENVD
                     KALRGILDLCKSLGFKTVYKDGYYGYAEIGQGEKMIGILGHVDVVPEGDLESWNYPPF
                     EAVLEDGKLYGRGTQDDKGPTISAIYAVKALMDLNVDFNKRIRFIFGADEENLWRCIN
                     KYKENNEEIPNYGFTPDSRFPITNAEKGLLQVHLTCDSKSDIELSVGKALNAVPGKAI
                     YIGKYSDKLKKELNKLNFEYTVEGNKICIIGKSVHSAASDTGINAVARLCIALNNIGI
                     DSNIIKFLAEVIGEDANGNNIIPNCKDDVSGKLTVNIGRVTIDNEKEFAGIDVRIPVT
                     YKKDDFVKELKKMTDKYNLNYEEYDFLDSIYVPEDTLLVKTLRKVYEEETGLDGTPLS
                     SGGATYARALDNCVAFGAIFPGKPETEHQANEYLIVEDIIKATQIYALSIYELLKI"
     gene            complement(4755..6035)
                     /locus_tag="PC471_002378"
     CDS             complement(4755..6035)
                     /locus_tag="PC471_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine gamma-lyase family protein"
                     /protein_id="HDF2789505.1"
                     /translation="MLNETKELLKDYYGIDDDTFKLSQEIMEEIKDKFEEIKEIREYN
                     QYKVLKAMQESKLSDMHFNWTTGYGYNDIGREKIEEIYSKVFNTEDALVRPIIVNGTH
                     ALTLCIQGIVRPGDEILSVTGRPYDTLEGVIGIREEKGSLKEYGVTYDDVDFLEDGNL
                     DLEGIKNKINDRTKLVMIQRSKGYSWRKSLSISDIKEAIEVIKSVKPEAIVMVDNCYG
                     EFLDTKEPTDVGADVMAGSLIKNPGGGLALTGGYIAGRKDLIELISYRMTSPGIGKEC
                     GLTFGTTRNVLQGFFLAPYIVSQAVMGAIFCSRAFEKLGYDVLPKYDDLRSDIIQCIR
                     LNNADEVISFCEGIQEAAPVDSYVKPVPWDMPGYESEVIMAAGAFIQGSSIELSADAP
                     IRPPYNVYFQGGLTFDHSKMGTLKAIEFIKKLKK"
     gene            complement(6162..6422)
                     /gene="hfq"
                     /locus_tag="PC471_002379"
     CDS             complement(6162..6422)
                     /gene="hfq"
                     /locus_tag="PC471_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HDF2789506.1"
                     /translation="MKNTVLNLQDLFLNNARKERIPVTIYLVNGVQVKGLVKGFDSYI
                     ILIEGDNRQQNMIYKHAVSTIQPGKYINLTNQNQNNNNNNNR"
     gene            complement(6536..7471)
                     /gene="miaA"
                     /locus_tag="PC471_002380"
     CDS             complement(6536..7471)
                     /gene="miaA"
                     /locus_tag="PC471_002380"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HDF2789507.1"
                     /translation="MKKIPLIILTGPTAVGKTDLSIKLAKDMNAEIISADSMQIYEYM
                     DIGSAKVTEEEMQGVKHYLIDEVKPDYPFSVSEFQQRAKKYIHEINKKEKCVLVTGGT
                     GLYLNSLIYNMDFAQSDANNELREELQKQLAEKGIDYMHNKLKELDEESANRIHKNNT
                     KRVIRALEVCLSGKKMNDFSSDLKFNEEYQPIIIVLNRDREHLYQRINMRVDIMLKNG
                     LVEEVKKLLSMGFKKDMISMQGIGYKEILKYLDGEYTYEEAIEIIKRDSRRYAKRQIT
                     WFKRYKTAKWFDLDQYENIDELKNEIILYIKDSIK"
     gene            complement(7509..9476)
                     /gene="mutL"
                     /locus_tag="PC471_002381"
     CDS             complement(7509..9476)
                     /gene="mutL"
                     /locus_tag="PC471_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HDF2789508.1"
                     /translation="MKNIINILDDLTINKIAAGEVVERPSSVVKELIENSIDAGANKI
                     SIDIIDGGKSLIKITDNGIGIPSSEVEKSFLRHATSKIKKIDDLYDLYSLGFRGEALA
                     SISAVSKLEMTTKTKDEIIGTKIYVEGGKIISKEPIGSTNGTTIIIKDIFFNTPARQK
                     FLKSTHAETINISDLINKLAIGNPNIQFKYTNNNKQMLNTPGDGKLVNTIRSIYGKEI
                     TENIIDVEFKCNHFKMNGYIGNNNIYRSNKNLQHIYINKRFVKSKIIIDAITESYKSI
                     IPIGKHAVCFLNIEVDPSCIDVNIHPNKLEIKFEKEQEVYIELRDFLKVKLIHSNLIG
                     KYATYSDKKTQPRIAINSREKSTDYKLRNNDLLESTPKNSNITKGKDEVIEVVTLSSE
                     KPINEFQSVSEVLNASVEDDVKNINYLSEDSANDNIQEEFQVDGIKNEGNYYLGDSIK
                     DSEEEYLCSSKRKFSLYGYSVIGVVFNTYIILSKDDSMYLLDQHAAHERILYERYMEK
                     FYRQDINMQILLDPVVIEVSNVDMLQIENNLELFMKFGFELEIFGNNHIMVRCVPTIF
                     GVPETEKFILQIIDNIEEITSNYDLKGERFASMACRSAIKANDKIYDIEIKSLLEQLE
                     KCENPFTCPHGRPIMVEISKTEIEKMFKRIM"
     gene            complement(9487..12330)
                     /gene="mutS"
                     /locus_tag="PC471_002382"
     CDS             complement(9487..12330)
                     /gene="mutS"
                     /locus_tag="PC471_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HDF2789509.1"
                     /translation="MQIDMNKLTPMMKQYLEVKNRYKDCILFFRLGDFYEMFFEDALV
                     ASKALEIALTGKACGLEERAPMCGVPFHSANSYISKLVENGYKVAIGEQMEDPSTAKG
                     IVRREVIRVITPGTVLDGNLLENKKNNYLLSLYKDGTNIGLTYVDISTGETNATCLNE
                     DKVIEEIAKIHPTEIIINDLDFIENLRDIATVSNIYINESFSDNYLDINILKEYFPDV
                     YLQKLKFDDKGLIKSSLSILLNYIYNTQKQITSNINNINIYNSSEYMVLDMFTRTNLE
                     LTQTIRGNKKKGSLLHVLDKTSTAMGGRLLRKYVEEPLINKSKIENRLDVIEEIKDDF
                     ILREDLNDILKNIYDIERICGKIAFERVTPKELIHLKNSIEKLPNLKDTINLSNAKIL
                     KEYVSEMDKLDDIYNLIDEAILEEPTITIKDGNIIKSDFSDELKELREISKNGAFLVK
                     EIENREREKTGVKSLKIGFNKVFGYYIEITKANFKQAKLDETYIRKQTLSNAERYITP
                     ELKEIEEKILHAEEKIKSLEYEIFVEIRDTIYKNIDRIQKVAKTIANIDVFVSLATVA
                     HINNYVKPAINENNKLDIRNGRHPVVENIVGEENFVPNDTYLNRGENIINIITGPNMS
                     GKSTYMRQTAIIALMAHIGSFVPAESADIPILDRIFTRVGASDDLSQGQSTFMVEMNE
                     VSLILKNATERSLVILDEIGRGTSTYDGISLAWSIVEYIQKNIRCKTLFATHYHELTD
                     LEEEFKEVKNYSIAVKEDGEGIIFLRKIIPQGADKSYGIYVAKLAKLPDEVIERAKYI
                     LKDLEKNHVYNSVAINGDKENNNIINSNLDEELVKVNQSNFKNKYESLKIEHELIVKD
                     YKHIKKDYDKLNSKFKALNDEVALIKQNDDKEKINQVDSVKEVALTQISFDSVNRDIL
                     SEEILNLDILNMTPLDAINSLYNLQRKAKEIKS"
     gene            complement(12490..13224)
                     /locus_tag="PC471_002383"
     CDS             complement(12490..13224)
                     /locus_tag="PC471_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2789510.1"
                     /translation="MLTQMNFYHNKKVKVSIENVSKTYKDIEVLKDISIDVYEGEFVS
                     ILGPSGCGKSTIFNIITKLTDYDSGKVNINGKYSYMYQKDLLLPYKTIIDNVSLPLIL
                     KKEKKSKARSMVKPYFEVFGLSGYEDKYPSELSGGMRQRANFLRTFINSNDIMLLDEP
                     FGALDSLTKTSMQKWLLDVKKKVNSTILLITHDIDEAIMLSNRIYVISKKPSIVKKEF
                     VIDSRKLNEDNLENIIKLKKEIISLL"
     gene            complement(13193..14206)
                     /locus_tag="PC471_002384"
     CDS             complement(13193..14206)
                     /locus_tag="PC471_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDF2789511.1"
                     /translation="MNFKKLGILGLVSVFTLATITGCSSNNNKDKNNDKDTSSKKVTM
                     VLDWTPNTNHTGLFVALDKGYYKEEGLDVEIVQPPESGAETLVATGKADFGISYQEQV
                     TYAKTSEDPLPIKAVATVIQHNTSGFASPKEKNITTAKDFEGKTYGGWGSPSEEAVFK
                     AVMKKNGADFNKLKIVNTGQDDFFAAMKTVDFAWIFEGWDAVKADLIGYDLNFIPVKD
                     LDERLDYYTPLIISNETVLKDNPELAKKFLKATTKGYEYAIKNPEESAKILVKHAPEV
                     DEKLALKSQEYLASKYKDDAPRWGEMKDSVWNNYTSFLKEYKLIDKDMKASDAYTNEF
                     LPQ"
     gene            complement(14228..14998)
                     /locus_tag="PC471_002385"
     CDS             complement(14228..14998)
                     /locus_tag="PC471_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2789512.1"
                     /translation="MKNLEIIDKVKDSIYPIATFLFILILWQSMVGVLEVPQYILPTP
                     VDIINVFFKDYQNLSMHAVVTIGEAILGFIVAIIISLVIGILMDFVSIIKKCLYPIML
                     VTQMIPTIIIAPLFMIWFGFGTMPKVLMVMLTCFFPILISFVDGIENIDKDYLNLFKT
                     MDSNKVNTFIHLKFPMAMDKFFSGLKISATYAVMAATVAEWLGGTKGLGVYMLRSKSA
                     YALDKVFASTILVVIFSLMFVGIVQAVKKVVIRHRLID"
     gene            complement(14973..15263)
                     /locus_tag="PC471_002386"
     CDS             complement(14973..15263)
                     /locus_tag="PC471_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MTH1187 family thiamine-binding protein"
                     /protein_id="HDF2789513.1"
                     /translation="MVIADIAVMPLRPYKGEEEMYKVVDACIAEIEKSGLKCEIGAMS
                     TTVEGDFDEVFELLKKVHKIPFNLGCERVITVARVDEKAGGLTIDEKLRNHR"
     regulatory      complement(15394..15493)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     regulatory      16113..16202
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            16255..16431
                     /locus_tag="PC471_002387"
     CDS             16255..16431
                     /locus_tag="PC471_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2789514.1"
                     /translation="MFMDKMDRCTHILTAYICSSRDYCNFIDTQLNDFILEYGENVVE
                     SCLHQVMVLVSRYN"
     gene            complement(16546..16902)
                     /locus_tag="PC471_002388"
     CDS             complement(16546..16902)
                     /locus_tag="PC471_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2789515.1"
                     /translation="MDIDNNFEEFPNIEERQKDFNCSIGFTMTVIGSKWRAIILWHIL
                     KQEPIRYSQLKKEVVHISHKILSQELKYLERDGLIKRISYPTIPPKVEYLSTKRGKSL
                     ESILLELCNWGKQYMR"
     gene            17369..18117
                     /locus_tag="PC471_002389"
                     /pseudo
     CDS             17369..18117
                     /locus_tag="PC471_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816229.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis isomerase"
BASE COUNT         5837 a         3072 c         1953 g         7330 t
ORIGIN      
        1 tattttcctt ctaaataaaa aatttgtcta aagtttctaa aaaatttaga caaatcttag
       61 gttattactc tttaaattat ttttaaatta tttttaaatt attccaaata cgccaaataa
      121 tattggtata aatatagctg gtaacaaatt ggctactttt atattcgtta atttaagtac
      181 atttagacca agtcctataa ttaataaact tccagctgct gtcatttctg tgactacagg
      241 atcacttaaa aatccattta aaaatgatgc tcccacacat ataatacctt catacaaaaa
      301 tacagatatt gcagaaaaca ttacacctat acctaaagaa gcagtaaata taattgaagc
      361 tattccatct ataacagatt ttacaaataa agtatcatga cttcctgtaa gaccactttc
      421 taatgaacca actactgcca tggctcctat acagaataat aaactagatg atataaaacc
      481 ctctgctata gaatcttttt tatttccttt agaaaattta ctttgtaaaa aagcacccaa
      541 cttatttacc catttgtcaa tatctattaa ttctcctatc agagcaccta cagctacgga
      601 tattattgtc actaatgtgt tttttccttc tagtgctcct gatataccaa tgtacaatac
      661 acataaggca attccattca ttatagtatc gcttactctt tgaggtaatc ctccttttat
      721 tataagacct actatacatc cacctgcaat tgctagacta tttgctatta ctccaaacat
      781 tttttctccc ctcgattagc taattgaaat ttatcctatt attaaaacct aaacatctat
      841 actttgtatt ggatatttta aatattccaa aaattcttca aaaccttttc tagatatatt
      901 tacagtttta gtatttatgt taggatgaaa atttaagtaa tcttctttaa ttacatcttt
      961 atctatgtaa agctcaactt ttctttctgt atcatttatt aatgaaaaag gatttacact
     1021 tccaggaaga agtttaagta cattgtatag tctttcaggt gaagcaaacg ataatctaga
     1081 acttcctatc ttatctttaa gtgagtttaa atcaacttgt ttttctcctt taactacaat
     1141 aagataatac ttatttcctt tggcatttct taaaaataaa tttttacatt gacacccctt
     1201 tgttattcca tctaaaatga ctaattgctc tgctgtatat acagcttcat gctctactac
     1261 atcatattca acatttaatt tatccaaaat atcatatatt ttagtttcat tcatgctaga
     1321 ttcattttca tcttgttctt ttttattatt taaatcactc atacttttct ccttaaacac
     1381 ttcatcataa tatcattgtg aggtattatt ctactatagc tattgctctt actggagctc
     1441 catcagcatc attatactta aatggtgctg ctataaatag aaatttttct ggaacatttt
     1501 ctaaatttgt aagattttct atgattattt ttcctttttc aaataaaata tgatgcactt
     1561 caaatactga tgtgtcatat ctatccactg atagcatatc tattcctatg ccttttatat
     1621 tagtattagc tatatagttt gcagcttctt ttgttaatgt aggataacct acatagtact
     1681 gctttttatc ccaaaatttt gaccaacctg atttaaatat tataaaatca ctatttttta
     1741 tttcatcttc atattttttt agatattcta attctatatt ctcatttccc ttttcaagtt
     1801 ccaatacata agctttccca acaaaatttt ctatatccaa cttatctagg gtctcacctt
     1861 tagtatacat atgtcttggt gaatccatat gagttccatt gtgagaaaat acactaatta
     1921 atgtttcttg ataaccattt tcttcaataa tagctacttt cttgatatct ggtctattag
     1981 gctctgcata tacaggcatg tcattatgtg tcacatgagt taaatcaaaa accttcatct
     2041 ttatataccc cctatgtttt tattttctat tatataccta aattcatttt atttctatca
     2101 gattttattc cattatatac catactacta tatatttttt ataaacctca agggatttac
     2161 cgaaaaatct tggaaagaat tatcagtaaa tataacatct tcacccaaaa aacgtttata
     2221 ccttccttct tctttcttta aagtaattct ctgtttttgt gaatgattat taaaaaccat
     2281 tatcttaaga ggaggttttt ttctattagt ctgcactctt tttaacatgt attttatgtg
     2341 catatcagct tcaggaaatg aatatccaca aaatattaaa aggtcagtat ctcttaaagt
     2401 tttttctgtt tcattccaaa cattacttaa aaatatgttt gacatattct taaaatatgt
     2461 tggtggcact ataataggtt ctgttaactc accacactct aaacattttg tctctgaact
     2521 attttcaata agcttcatga caccaccttc atgtggtgta agagttacac tattacaaat
     2581 ggggcaataa agccaattca atgacccatg tatcttatat aatttgatta atggttcttt
     2641 tggttttctc caatttaact cctctttaaa atttgtaaac tccacaccat aatcaagcat
     2701 aattggcaaa tcattcttat ctcttaatcc agtaactgta ttgtctatat gtatatcata
     2761 atttgcacta ataaaagtag tatcaagaag caaatcattt tccaaaagat tttcaactaa
     2821 aagtttatga tatttattat ctgttctagg tgtattatga attgcttcag ccattagcag
     2881 tattaaatat tgtcttaaaa aacgtatact atcacttctg ttatttagat tttctagtcc
     2941 aaaatttcta aatgcttctt ttctttgttc tgctaagtct aaaagaccta aaacttcttc
     3001 aaaggtagga aaatttgcat tatctatatt atcttttact atatcaatat tgaacatctg
     3061 tttaaagaaa atatacaatt ctctattcat tttggtatta taatttgtgg gatttagttt
     3121 tttaaaatat tcgctaaaaa gttcattttg aataggtaga ttctctgctg ctgaagctcc
     3181 tgctcctaaa aatattgcta ttttcatata gttcctccta atataaatat tactcctatt
     3241 tttatatcca ttaaatatcc atatcattta ttttatgata caacaataaa gctgtctttt
     3301 actagaaaaa atcctattac aagacagcct tatattatat taaatcttta ataattcgta
     3361 aatagaaaga gcatatattt gagtagcttt tataatatcc tctactatta agtattcatt
     3421 agcttggtgt tctgtttctg gttttcctgg aaaaatagct ccaaatgcaa cacaattatc
     3481 taaagctctt gcgtatgttg ctcctccaga tgaaagtggt gttccatcaa gaccagtttc
     3541 ttcttcatat acttttctaa gtgtcttaac aagaagagta tcttctggaa catagattga
     3601 atccaagaaa tcatattctt cataatttaa attgtattta tctgtcattt tctttaactc
     3661 ttttacaaaa tcatcctttt tgtatgttac tggaatcctt acatctattc ctgcaaattc
     3721 tttttcgtta tcaatagtta ctctacctat atttactgtt agtttacctg atacatcatc
     3781 cttgcagtta ggaattatat tgtttccatt agcatcttca ccaataactt ctgctaaaaa
     3841 cttaattata ttagaatcta tacctatatt attaagagct atacaaagtc tagccactgc
     3901 atttatacca gtatcacttg ctgctgaatg gacactcttt ccaattatac agattttatt
     3961 accttcaaca gtatactcaa aatttaattt attaagttct ttttttaatt tatcagagta
     4021 ttttccaata tagattgctt tacctggaac tgcatttaaa gcctttccaa ctgacaactc
     4081 tatatcactt ttgctatcac aagtcaaatg aacttgtaaa agacctttct ctgcatttgt
     4141 tattgggaat ctagaatctg gagtaaatcc atagtttggt atttcttcat tattttcttt
     4201 atatttattt atacatctcc aaagattttc ttcatcagcc ccaaaaatga atcttatcct
     4261 cttattaaag tctacattta aatccataag agctttcact gcataaattg ctgatattgt
     4321 aggtccttta tcatcttgtg ttcctcttcc atacaattta ccatcttcta aaacagcttc
     4381 aaatggaggg taattccagc tttctaaatc accttctgga accacatcaa cgtgaccaag
     4441 tatacctatc attttttcac cttgtcctat ttctgcatat ccataatatc catctttgta
     4501 tactgtctta aatcctaaag atttacataa atctaatata cctcttaaag ctttatctac
     4561 attttcacca aatgggcaat tttcagttgc ttcagatata acactaggta tttttacact
     4621 ttcttgtatt gaagaaatca tttcatcttg tagagaattt actttttctt taatttgctc
     4681 tttcatatta tcacccttca attttcatca taatatacta ataaatattt tgacctttta
     4741 aaaaaaaaat atgcttattt tttcagtttt tttataaatt caatcgcttt taaagtcccc
     4801 atttttgaat ggtcaaatgt aagtccacct tggaaataaa cattataagg aggtcttatt
     4861 ggagcatctg cacttaattc tatagaagaa ccttgtataa atgctcctgc tgccattatg
     4921 acttcacttt cgtatccagg catgtcccaa ggaacaggtt ttacataaga atcgactggt
     4981 gcagcttctt gtataccttc acaaaaactt attacttcat cagcattatt aagtctaata
     5041 cattgaataa tatcacttct taagtcatca tattttggta atacatcata tcctaatttt
     5101 tcaaaagctc ttgagcaaaa gatagctccc attacagcct gagatactat ataaggcgcc
     5161 aagaaaaaac cctgaagtac atttctagtt gttccaaaag taagaccaca ttcttttcct
     5221 attccaggag aagtcattct ataagatata agctcaatca aatcttttct accagctata
     5281 tatccacctg tcaatgcaag cccaccacca ggatttttta ttaaagagcc agccataaca
     5341 tctgctccaa catcagtagg ctctttggta tctaaaaact caccataaca attatcaacc
     5401 attactatag cttctggttt aacagacttt attacttcta tagcttcttt tatatcacta
     5461 attgaaagtg attttctcca agaatatcct tttgaccttt gtatcataac gagtttagtt
     5521 ctatcattta ttttgttttt aattccttct aaatctaagt ttccatcctc taaaaaatct
     5581 acatcatcat atgtaacacc atactctttc aatgaacctt tttcttctct tatgcctata
     5641 actccctcta aagtatcata tggtctacca gtaacagata aaatttcatc tcctggtcta
     5701 actatacctt gtatacataa agtaagtgca tgtgtaccat ttacaattat tggtcttaca
     5761 agagcatctt ctgtattaaa tactttagaa taaatttcct ctattttttc acgaccaata
     5821 tcattatatc catatcctgt agtccagtta aagtgcatat cacttagttt agattcttgc
     5881 atagctttta aaactttata ttgattatat tctcttattt cttttatttc ttcaaactta
     5941 tcttttattt cttccattat ttcttgcgat agtttaaaag tatcatcatc tattccatag
     6001 tagtctttta ataattcttt tgtctcatta agcattttat cacctgtctc tttattattt
     6061 tatttaaaca tgtaaaggag actattttac taaaacaaat aaattatagt ttataagtta
     6121 gtataagtct cctctcaatc atcttaaatt aaattaatta tctatctgtt gttattatta
     6181 ttgttgtttt ggttttgatt tgttagatta atatactttc caggttgtat agttgataca
     6241 gcatgtttat aaatcatgtt ttgttgtcta ttatcccctt ctattaatat tatataacta
     6301 tcaaacccct ttacaagccc tttaacttgc actccattaa ctaaatatat agtaacaggt
     6361 atcctttctt tccttgcatt atttaaaaac aaatcttgta aatttaaaac tgtatttttc
     6421 attagatttg taccctccca agattaaatt gaacttctaa aatattccta ccttatattc
     6481 tatataagtt atgacaaatt ttcaatatta agttgtatac ttaaaaattt atatattatt
     6541 ttatagaatc tttaatatat aagattattt cattttttaa ttcgtctata ttttcatatt
     6601 ggtctaaatc aaaccatttt gctgttttat atcttttaaa ccatgttatc tgacgttttg
     6661 cataccttct agaatctctt ttaattattt caatagcttc ttcgtatgta tattctccat
     6721 ccagatactt taatatctct ttataaccta taccttgcat tgatatcata tcttttttaa
     6781 atcccatact taaaagtttt tttacctctt caaccaatcc atttttaagc ataatatcaa
     6841 ctctcatatt tattctttga tacaaatgtt ctctatctct atttagaact attattattg
     6901 gttgatattc ttcattaaat tttaaatcac ttgaaaaatc attcattttc ttaccactta
     6961 gacaaacttc aagagctctt atgacccttt tagtattgtt tttatggatt ctattagcag
     7021 attcttcatc aagttctttt aacttattat gcatgtaatc tattcccttt tcagcaagtt
     7081 gtttttgaag ttcttctctt aattcattat tggcatcaga ttgtgcaaaa tccatattat
     7141 atattaaaga gtttaaataa agtcctgtgc ctccagttac taaaacacat ttttcctttt
     7201 tatttatttc atgtatatat ttttttgctc tttgttgaaa ctctgaaact gaaaatggat
     7261 agtcaggctt aacttcatct ataaggtaat gcttcactcc ttgcatctct tcctctgtaa
     7321 cttttgcact tccaatatcc atatattcat atatttgcat agaatcagct gaaattatct
     7381 ctgcattcat atcttttgct aattttattg acaaatctgt ttttccaaca gcagttggac
     7441 ctgtaagtat tataagtggt atttttttca taacattcct ttccatatat tttaaatatc
     7501 caaatatttt acataattct tttaaacatt ttttctattt ctgtcttaga tatttcgacc
     7561 attataggtc ttccatgagg acatgtaaat ggattctcac acttttctag ctgttccaat
     7621 aaacttttta tttcaatatc gtatatttta tcatttgctt ttatagcaga cctacatgcc
     7681 atagatgcaa atctctctcc ttttaaatca taattacttg taatttcttc tatattatca
     7741 attatttgaa gtataaattt ctcggtttca ggtactccaa atatagtagg aacacatctt
     7801 accattatat gattattgcc aaaaatttct agctcaaaac caaatttcat aaataactcc
     7861 aagttatttt caatttgaag catatctaca tttgatactt caataaccac tgggtctaaa
     7921 agtatttgca tatttatatc ttgtctatag aacttctcca tatatctttc atatagtatt
     7981 ctttcatgtg cagcatgttg gtccaacaaa tacatagaat cgtctttgct caaaattata
     8041 taagtattaa atacaactcc aattacagaa tatccgtata aagaaaactt gcgttttgaa
     8101 ctacacaagt attcttcttc actgtcctta atactatctc ctaagtaata atttccttca
     8161 tttttaattc catcaacttg aaactcttct tgtatattat catttgcact atcttcactt
     8221 agataattaa tgttttttac atcatcttct acacttgcat ttaggacttc acttactgat
     8281 tgaaattcat ttattggttt ttcacttgat aaagtaacaa cttcaatgac ttcatcctta
     8341 cctttagtaa tattactatt tttaggagta gattctaata aatcattatt tcttaattta
     8401 taatctgtac ttttttctct actgttaata gcgattctag gttgtgtttt tttatcacta
     8461 taagtagcat attttccaat aagatttgag tgtattaatt taacctttaa aaaatctcta
     8521 agttctatat acacttcttg ttctttttca aatttaattt caagtttatt ggggtgtata
     8581 tttacatcta tacatgatgg gtctacttct atatttaaga agcacactgc atgtttacct
     8641 attggaatta tagacttata actttctgtt attgcatcta tgattatttt actttttaca
     8701 aatcttttat ttatatagat atgttgtaag tttttatttg aacggtatat attgttatta
     8761 cctatatatc cattcatttt aaagtgattg cacttaaact ctacatctat tatattttcc
     8821 gttatttctt ttccatatat acttctaata gtgtttacaa gctttccatc ccctggagta
     8881 tttagcatct gtttattgtt atttgtatat ttaaattgaa tgtttggatt acctattgca
     8941 agtttattta tcaaatcact tatatttata gtttctgcat gagtagattt taaaaatttc
     9001 tgtcttgctg gtgtattaaa gaaaatatct tttataatga tggtagttcc attagtagaa
     9061 cctattggtt ctttcgatat tatttttcca ccttcaacat atattttagt tcctattatt
     9121 tcatctttag ttttagttgt catttctaat tttgatacag ctgaaataga tgccaatgct
     9181 tctcctctaa aaccaagaga gtataaatca tacaaatcat caattttttt aattttacta
     9241 gtagcatgtc tcaaaaatga tttttcaact tcacttgaag gtattcctat tccattatct
     9301 gtaatcttaa ttagactttt tcctccatca attatatcta tggaaatttt atttgcacct
     9361 gcatcaatgg agttttcaat aagttctttt actacagaag aaggtctttc tacaacttca
     9421 cctgctgcta tcttatttat agtcaaatca tctaggatgt taattatatt tttcattttt
     9481 acacctctaa gattttattt cttttgcttt tctttgaaga ttataaagag agtttatagc
     9541 atctaatgga gtcatattta aaatatccag atttaaaatt tcttctgata gtatatctct
     9601 atttacacta tcaaaagaga tttgagttaa ggctacttct tttacagaat ccacttgatt
     9661 aattttctct ttatcatcat tttgctttat taaagcaact tcatcattta atgctttaaa
     9721 tttagaattt aatttatcat aatccttttt tatatgtttg tagtctttta ctataagttc
     9781 atgttctatt tttagagatt catatttatt cttaaaatta ctttgattta ccttaactaa
     9841 ttcttcgtcc aaattactat taattatatt attgttttct ttatctccat ttattgcaac
     9901 actgttgtaa acatgatttt tttccaagtc tttaagtata tattttgctc tttctatgac
     9961 ttcgtctggt aatttagcta atttagcaac atatattcca taactcttgt ctgctccctg
    10021 aggaattatt tttcttaaaa atattattcc ttccccatct tctttgactg ctattgaata
    10081 atttttaacc tctttaaact cttcttctaa atcagtaagt tcatgataat gtgttgcaaa
    10141 taatgtctta catctaatat ttttttgaat atattccact attgaccatg ctaaacttat
    10201 accatcatag gtactagttc cacgacctat ttcatctaat ataactaaac ttctctctgt
    10261 tgcatttttt aatataaggg atacttcgtt catctctacc ataaaagtag attgaccttg
    10321 agataaatcg tcactagctc caactcttgt aaatattcta tctaatattg gaatatcagc
    10381 agactctgct ggtacaaatg agcctatatg tgccataaga gctattatag cagtttgtct
    10441 catatatgtt gatttacctg acatattagg accagttatt atgttaataa tgttttctcc
    10501 acgatttaaa taagtatcat ttggaacgaa attttcctct cctactatat tttcgacaac
    10561 aggatgtcta ccatttctta tatcaagttt attattttca tttattgctg gttttacata
    10621 gttatttata tgtgcaactg ttgcaaggga cacaaaaaca tctatattag ctatagtctt
    10681 agccactttt tgtattctat ctatgttttt atatattgta tctcttatct ctacaaatat
    10741 ctcgtattct aaagatttaa tcttttcctc tgcatgtagt atcttttctt ctatttcctt
    10801 taattctgga gttatatatc tttctgcatt actaagtgtt tgctttctta tatatgtctc
    10861 atctagcttt gcctgtttaa agttagcttt tgttatttct atataatatc caaaaacttt
    10921 attaaagcct atttttaatg atttaactcc tgttttttct ctttccctgt tttctatttc
    10981 ctttacaaga aaggctccat tcttagaaat ctctcttagt tccttaagtt catcactaaa
    11041 atctgattta attatgtttc catcttttat ggttatcgta ggttcttcta gaatagcttc
    11101 atctattaaa ttgtatatat catctaattt atccatttct gaaacatact cttttaggat
    11161 ttttgcattt gataaattta tagtatcttt taaattaggt aacttttcta tcgaattttt
    11221 taagtgaatt aactcttttg gtgttactct ctcaaatgct atctttccac atattctttc
    11281 tatatcgtat atatttttaa gtatatcatt taggtcttct cttaatataa agtcatcttt
    11341 tatttcttct attacatcta atctattttc aattttagat ttatttatca aaggttcttc
    11401 tacatatttt cttaaaagtc tacctcccat tgctgtagaa gttttatcaa gtacgtgtaa
    11461 taatgaacct tttttcttat tccctcttat agtttgagta agttctaaat tagttctagt
    11521 aaacatatct agaaccatat attctgatga attgtatata tttatattat taatgttaga
    11581 tgttatttgt ttttgagtat tgtatatata attcagaaga attgataagc ttgactttat
    11641 tagaccttta tcatcaaact ttagtttttg tagataaaca tctggaaaat attcttttaa
    11701 tatatttata tccaaatagt tatcactaaa actttcattt atgtatatat tacttacagt
    11761 agctatatct ctaagatttt ctataaagtc taaatcattt ataattattt cagttggatg
    11821 gatttttgca atttcttcaa ttaccttatc ttcatttaaa catgttgcat tagtttctcc
    11881 tgtacttata tcaacataag ttagacctat attagtacca tccttataca aagataataa
    11941 ataattattc tttttatttt caagtaaatt tccatctaaa actgtaccag gagtgatgac
    12001 tcttataacc tctcttctaa caattccttt tgctgtagat gggtcttcca tttgttcacc
    12061 tattgctact ttatatccat tttcaactaa ttttgatata tatgaattgg ctgagtggaa
    12121 aggaacacca cacataggag cacgttcttc taaaccacaa gcttttcctg tcaatgctat
    12181 ttcaagagct tttgaagcta ctaatgcatc ttcaaaaaac atttcataaa aatctccaag
    12241 tctaaaaaac agtatacagt ctttatatct attttttact tcaagatact gtttcatcat
    12301 aggagttaat ttattcatat ctatttgcat tatcaaacct ccttcttttc taattaaata
    12361 ttatatcata gtagatgatt attattaaca caatatataa aattttgaca tataactaaa
    12421 aacaatccaa aatgtatact cacattttgg attgctaagt ttaatgctta ttaaataaaa
    12481 atattttttt tacaataaag atattatttc ttttttaagt ttaatgatat tttcaaggtt
    12541 atcttcattt aactttctac tatctataac aaattctttc tttactattg atggtttttt
    12601 tgaaataaca taaattctgt ttgatagcat tatagcttcg tcaatatcat gagttataag
    12661 aagtattgtt gaatttactt ttttcttaac atctaaaagc catttttgca tggatgtttt
    12721 agttaatgaa tcaagtgccc caaaaggctc atctaaaagc attatgtcat tagaatttat
    12781 aaaagttctc aagaagttag ctctctgtct cattcctcca gaaagttctg aaggatattt
    12841 atcttcatat ccgcttaatc caaacacctc aaaataaggt tttaccattg acctagcttt
    12901 actctttttt tcttttttta aaattagtgg tagtgacaca ttatctatta tagttttata
    12961 tggcaagagt aaatcttttt gatacatata actatactta ccatttatat tcactttgcc
    13021 actatcataa tctgtaagtt tagttataat attaaaaata gtactttttc cacatccact
    13081 tggccctaat atagaaacaa actcaccttc ataaacatca atacttatat cttttaaaac
    13141 ttcaatatct ttataagttt ttgatacatt ttctatagat acttttactt ttttattgtg
    13201 gtaaaaattc atttgtgtaa gcatcacttg ccttcatatc cttatctatt aatttatatt
    13261 cttttaagaa tgatgtatag ttgttccata cactatcttt catttctccc catcgtggtg
    13321 catcatcttt gtacttgctt gctaaatatt cttgactctt taaagctagt ttttcatcta
    13381 cttcaggtgc atgttttact aaaatttttg ctgattcttc tggatttttt attgcatatt
    13441 cataaccttt tgttgtagct tttaagaatt ttttagcaag ctctggatta tctttaagca
    13501 cagtttcatt agaaattata agtggtgtat aataatccaa tctctcatct aaatctttta
    13561 ctggtataaa gtttaagtca tatccaatta aatcagcttt aactgcatcc caaccttcaa
    13621 atatccatgc aaaatctaca gttttcatag ctgcaaagaa atcatcttga ccagtattaa
    13681 ctatctttaa cttattaaaa tcagctccat ttttcttcat aactgcttta aaaacagctt
    13741 cttctgatgg agagccccaa ccaccataag tttttccttc aaaatcttta gctgttgtta
    13801 tattcttttc ttttggtgat gcaaacccag aagtattatg ttgtataaca gttgctacag
    13861 cttttattgg aagtggatct tcactagtct ttgcataagt aacttgttct tgatagctta
    13921 taccaaagtc agcttttcct gttgctacta aagtttctgc tccactttca ggtggttgta
    13981 ctatttcaac atccaaacct tcttctttat agtaaccctt atcaagtgct acaaataatc
    14041 cagtatgatt tgtatttggt gtccaatcaa gcaccatagt tactttttta gaagaagtat
    14101 ctttatcatt gtttttgtct ttattgttat ttgaagaaca tccagttatt gttgccaatg
    14161 taaaaacact tactaaacct aatattccta actttttaaa attcataatt gcctccctca
    14221 aataccttta atctattaat ctatgtctta taacaacttt ttttacagcc tgtacaattc
    14281 ctacaaacat taaactaaat ataactacta atatagtaga tgcaaatact ttatcaagtg
    14341 catatgcact ttttgaccta agcatataaa caccaagtcc tttcgttcca cctagccatt
    14401 ctgcaacagt tgctgccatt acagcatatg ttgcagatat tttaagtcca gaaaaaaatt
    14461 tatccattgc cataggaaat ttaaggtgaa tgaatgtatt aactttattt gagtccatag
    14521 tcttaaataa atttaaataa tctttatcaa tattctcaat gccatctaca aaacttatta
    14581 atataggaaa aaaacaagta agcattacca tcaatacttt tggcattgtt ccaaatccaa
    14641 accatatcat aaagagtggt gctataatta ttgtaggtat catctgtgta accaacataa
    14701 taggataaag acattttttt attattgata caaaatccat caatattcca attaccaatg
    14761 atattataat tgcgactata aaaccaagta ttgcttctcc tatagtaacc acagcatgca
    14821 tggataaatt ttgataatct ttaaaaaaca cattaattat atctactggt gtgggtagta
    14881 tatactgagg aacttctaaa actcctacca tagattgcca aagaataagt ataaacaaaa
    14941 atgttgctat tggatatata ctatctttga ctttatctat gatttctaag tttttcatct
    15001 atagtcagac ctccagcttt ttcatctact ctagctactg ttatgactct ttcacatcca
    15061 agattaaaag ggattttatg tacctttttt aataattcaa aaacttcatc aaaatcacct
    15121 tcaacagtag tgctcattgc tcctatttca catttaagac cacttttttc aatttcagca
    15181 atacaagcat ctactacttt atacatctct tcttcacctt tgtaaggtct tagtggcatt
    15241 actgctatgt ctgcgattac catttaaatc actcctttaa aaataattaa tacctactag
    15301 gtatattttg ttttttatta aaaaatttta ttactaaaaa gatattatat ttaaataaaa
    15361 gaaaagtctt atccaaaata ggataagact tatttttatc ttaactggtc tccctacact
    15421 ggcattatcc aaacaggttc aaagggtcga gataaaatct cctctcagca cattagctcc
    15481 ccgaaatata ttatatcttt tctacttgat tatactacac ttatcatatt ttaacaaatt
    15541 ttttatttat attagatatt ttatccgttc aaaaatatct aatataaatt gtacattatt
    15601 acaccaaata aattatctta aaatggtatt ttatcaacat cttccaaaac tagattaact
    15661 gaattttcta gtttttattt tgctatttca agcatatttg gatgtatatc aattccatac
    15721 aaatattgtg ctaaatttct gttttcactc atctaatttg caatcgttac agattacaga
    15781 ttagaaacag tttttagact ctacttatct ttatctacaa aaaatctttc aataaattca
    15841 ttaataatat tcagtaatct aaaaataaac ctctatctgg atttttaaga tacaaacctt
    15901 ttaggaagta tcttttgtga taatataaaa attactcgtc attatcatca aaatcctcag
    15961 caaccaacat tttatataag aatcaaactc tataatataa ttattttatc caatttattt
    16021 ttgagaaata aaatattttt ttaattttta aattttttaa tatgcacctt gaaaaaagaa
    16081 acgttttcct atattatggt aatagtagaa taaaactaaa taggcaaaac tagagaaatt
    16141 tagtgacgca aagctatagg gactaagact tatgaaatga gttatgtcag ccagttgcca
    16201 aagagatatt ttctttttgt ttttatgaat gttttttagg gggatatatg atatatgttt
    16261 atggataaaa tggatagatg tacacatata ctaactgcat atatatgcag ttcacgcgat
    16321 tactgcaatt ttatagatac acaattaaat gattttatac tagaatatgg agagaatgtg
    16381 gtagaatctt gtttgcatca agtgatggta ttggtaagca gatataatta aagaaatttt
    16441 attttctaga aaaccctttt tagttttatc tacgttttat aattcattaa agaactaatt
    16501 aaaataagct tcattatata tttttcatga agtaaacagt taagtttatc tcatatactg
    16561 tttaccccaa ttacataatt ctaataatat agattccaat gatttaccac gtttagtaga
    16621 caaatattca acttttggag gaatagtcgg ataagaaatc ctcttaataa gtccatctct
    16681 ttcaagatac tttaattctt gtgacagtat cttatgactt atatgaacaa cttctttttt
    16741 taattgactg taacgtattg gctcttgttt taaaatatgc cataaaataa ttgccctcca
    16801 tttacttcct atcacagtca tggtaaaacc aattgaacaa ttaaaatctt tttgtctttc
    16861 ttcaatattt ggaaattctt caaaattatt atctatatcc atctcaatcc tctctttcaa
    16921 aatagtaagt atctaactaa aaagtgcatt cttgtttagc gtactaattc gtaatataat
    16981 cttgattaaa aaatagctat gcacactcgt gactttagtc atgggaggtt cagtgtagca
    17041 tagagcctta cataatacta cttttttaga tgtatatgtt taaattttaa tatagacata
    17101 tgttatttta ttaattttat ttaaacagat aataataaat aataatttat ctcaagtttt
    17161 ctatgtagat aaactaatct aatgtttaga aaggattata taatatgaat aaaaaatttc
    17221 gtctttatca agttgactca ttacaaaaga aaaatttaca ggtaatccag ctggtgtaat
    17281 tacaaatgct gatggcttat ctgaatcaca aatgcaaaaa atagcaagag aattaaataa
    17341 ttcagaaact gcttttattt tcaataactt ggctaaaaat catcaagaaa attatgatgt
    17401 acatatcaga ttttttacac caactaatga ggttcctata tgtggtcatg ctactattgc
    17461 tgcccattat gttagagcaa tagaaaacca attcaataac aaaacagtat ttcaaaaaac
    17521 tggtgctgga atattaccag ttgatattat aaaaaaagat aatgattatg aaataattat
    17581 gacacaagga aaaattgaag tatctccacc attacaacaa acctatcaaa aaagattact
    17641 taatgcattt ggtatttaaa ggaagatatt cgtacagatt gtcctattgc catatcatct
    17701 acaggacatt caaaagttat gattggaata aaggaattat caacattaaa tagtttatct
    17761 ccaaatttat atgaattatc gaaaataagt caagaaataa aatgtaatgg atattatatt
    17821 tttacattac ataataaaga aactctacaa gttcatggaa gaatgtttgc tcctgcaatt
    17881 ggtatttcag aagaccctgt tacaggaaat gctaatggac ccttaggtgc gtaccttgta
    17941 cattataaag taatacctac acccaacaca gagtttatat ttgaagcagt tcaaggagaa
    18001 gcaataaaac gcaaaggcaa gatgttagta aaagtaatta ttttagataa taagccagtt
    18061 gaagtaaaaa taattggaaa agctgtaatt gcattctcaa caaacataga aatctaatta
    18121 tagattcctt ctaagtaaat ttttgaactt tttaatacta atttaataac aaactaattt
    18181 atatacaatc aa
//
LOCUS       DAMJYR010000072        18190 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.072, whole genome shotgun sequence.
ACCESSION   DAMJYR010000072 DAMJYR010000000
VERSION     DAMJYR010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 18190)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18190)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18190
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.072"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            452..2122
                     /locus_tag="PC471_002390"
     CDS             452..2122
                     /locus_tag="PC471_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF and EAL domain-containing protein"
                     /protein_id="HDF2789516.1"
                     /translation="MREESNSLSLDHIEIFFELLSQSTEDYIFFWDINRNKFKISSAI
                     FDEFNLSKEIESDLVNCWSKIVYPDDVQIWKDDIQELLHGKKGEHNLEYRLINKYKEI
                     VWISCRGKVYVSDDPKTIFLVGRIKNIGEKNKFDSITGTWNREQFEHRMNYLIKEKIY
                     KNGAMFIMDIDNFKNINEKYGHSYGDKVLRAIATEVLEYLPKDVRLYRLDGDEFAFFY
                     PMCTKETIEKIYEKIQMYTNTQHEIESNKYYCTVTAGVAMYPEDGDNYLDLFKHADIA
                     LDIAKISGKNRIKFFSQELYENKLKVISMQQKLRECVENNFNDFELFFQPQVNAVTKE
                     VIGAEVLLRWHSSTYGEVSPVEFIPILEQSNLIIPVGKWIIKEAVKQCKEWHKINPDF
                     KISVNVSYIQLKEDFFRDFIVECLVEYQLRPEFLILELTENCWIPDINLLNDKFISLK
                     GIGVYIAIDDFGTGYSSLNYLKELSVNIIKIERSFVKNITYNSYEYTFLEYIIKLAHI
                     INLKVCVEGIESYEEYDIVKSLGVDIIQGFLFGRPVSASEFYKFQLSK"
     gene            complement(2244..2978)
                     /gene="pflA"
                     /locus_tag="PC471_002391"
     CDS             complement(2244..2978)
                     /gene="pflA"
                     /locus_tag="PC471_002391"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HDF2789517.1"
                     /translation="MIKGKIHSIETFGTVDGPGIRYILFFQGCPLRCKYCHNRDTWDI
                     KSGKEYTVDEIITDALKYTSFMKFSGGGITASGGESTLQPEFLSELFKKAKENDIHTC
                     LDTSGFVDIETIDPVLDNIDLVLLDLKHMVEEKSIDLTGVGMDKALKLARHLESRNIP
                     VWIRHVLVPGITDDIDNLEKLGQFVATLKNVERFELLPYHSMGIHKWESLGIDYELKD
                     VPDATKEDVQKASEIISKFGVKVYNS"
     gene            complement(3138..5369)
                     /gene="pflB"
                     /locus_tag="PC471_002392"
     CDS             complement(3138..5369)
                     /gene="pflB"
                     /locus_tag="PC471_002392"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HDF2789518.1"
                     /translation="MNAWQGFKTGRWTKEINVREFIQLNYSPYEGNDSFLAGATENTK
                     KLWDEAMVLFKKERENGGTLDVDTKTVSGIAAYAPGYLDKELETIVGLQTDAPLKRAV
                     MPYGGIKMVENSCEAFGYELDPEIKDIFTKYRKTHNQGVFDVYTPEMRAARKSGIITG
                     LPDAYGRGRIIGDYRRVALYGVDALIEDKNEQKKSLEVSCMDEEVIRLREEITEQISA
                     LNELKKMAESYGFDISKPATNSKEAVQWLYFGYLGAVKDQNGAAMSLGRTSTFLDIYF
                     ERDLKAGIVTEEELQEYMDHFVMKLRMVKFLRTPDYNNLFSGDPTWVTECIGGMGIDG
                     RTLVTKNSFRMLNTLYTLGPSPEPNLTVLWSTKLPQGFKDFCSKVSIDTSSVQYENDD
                     LMRAYWGDDYGIACCVSAMRIGKQMQFFGARVNLAKTLLYAINGGVDEKSGVQVGPRF
                     EPITSEYLDYDEVMSKFEPFTDWLATLYVNTLNVIHYMHDKYSYEALEMALHDRDIFR
                     TMACGMAGLSVCADSLSAIKHAKVKTIRNEQGIAVDFEIEGDYPKYGNNDDRVDSIAV
                     ELVESFMNKIRKNKTYRNSYPTQSILTITSNVVYGKKTGNTPDGRRAGAPFAPGANPM
                     HGRDTNGALASLSSVAKLPYEHAQDGISNTFSIVPGALGKDMTERINNLSAMMDGYFA
                     QNAHHLNVNVFDRATLEDAMEHPEEYPQLTIRVSGYAVNFIKLTKEQQLDVINRTFHG
                     KMA"
     gene            5662..6723
                     /locus_tag="PC471_002393"
     CDS             5662..6723
                     /locus_tag="PC471_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HDF2789519.1"
                     /translation="MEKRKSIHLHKLNIYLIGGGDRLFITVVLFLVGFLLITKGADIF
                     INCTVEIGKKTNISEIILGATIVSFATTLPEFTVSLLASIDGHTTMSLGNAVGSIICN
                     TGLALGLVVFISPFNVDKKMFFSKSLLLIVSVIVLILLSLDGVITRGDSLLLIIILIF
                     YMVNNYRSVVGKSDTKNRNIKNNTIKDISTKKGKNYRGFSVLEIVKILLLFATGLIMM
                     IIGSQILIESGVIIASFLNIPQGIVSLTIIALGTSLPEIVSSITAIRKNHHEISVGNI
                     LGANILNIVSVIAVSAIPNNIPILSQNRQLDIPFMILLLLIVIIPTLKSNKLSRIQGI
                     LMLFTYFLYISILYFMYII"
     gene            6814..8427
                     /locus_tag="PC471_002394"
     CDS             6814..8427
                     /locus_tag="PC471_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HDF2789520.1"
                     /translation="MNITKFEDLPISEGIKKAIAEMGFEEPSPIQAQSIPAILSGKDV
                     IGQAQTGTGKTAAFSIPILETIDPNNRSLQAVVLCPTRELAIQVSTEIRKLAKYSHGI
                     KTLPIYGGQPIDRQIKSLKSGVQVVIGTPGRTIDHINRKTLKMDNVKMIILDEADEML
                     DMGFREDIEMILSKIPEERQTTFFSATMPRGILELTKRYQKDPEHIKVVRKELTVSNT
                     KQYYIETRSSNKLEVLCRLVDVYDPKLSVVFCNTKRKADELVGDLQARGYFADALHGD
                     LKQTQRDIVMDKFRNGTIDILVATDVAARGIDVDDVECVFNYDLPQDEEYYVHRIGRT
                     GRAGREGMSFTFVFGKEMRKMKDIERYTKSKLIKHNIPTITDVEEKKVGTFFAQVKQT
                     IEEGHLTKQLQWLEGFCNDEDYAMVDIAAALVKLSLGEEMKEEIIEEKPRRERGDRKG
                     GTGAKDGMIRLFINIGRNQRVQAKDIVGAIAGEVGIPGKVVGTIDIYDKYTFVEIPKK
                     DAKTVIEKMKDIKIKGNKINIEKANKKKK"
     gene            8702..10687
                     /locus_tag="PC471_002395"
     CDS             8702..10687
                     /locus_tag="PC471_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HDF2789521.1"
                     /translation="MSMTYMDPRVFLILEMVLEEDTNTLEQFSNVLGVSTRTIRNYIN
                     QINRELKGGIAKIVKNEKGTYSIRIEDESKLIEIVNFNRGKLSNFINLNSPDERINYV
                     LDILTMTNNPITIDDLAEEICIGRTTLIKDLKKADRILAEYDLELKRKPNIGMILVGE
                     EMDIRLLILDRLYENYIDVLENVNQINLVKDYDIECLRDELKKLFKKEELYITEQVLR
                     DVERYIIMSVLRNLNGYKFEKIDKRFEVIRCSDEYTLGLKLKALLEKIFNIVLNEKET
                     IFLTMPLIGRKAPSTKLALSSIKINDSVKEVVDEVTSFLLETSGIDFKEDKKLIENLE
                     YHLYFALNRMRFNIRVKNPLLQEIKERYTFPYSVAKIAAMIIEEKFDLKICDHEIGYI
                     ALHFGSYFEKNSRKVLNVNRVAVVCGTGLGTAQLIRIKIGKILGESVEVNTFSDIEAK
                     ANLDLLKYYDLVFTTVDFKTDLDVPIFRVNALFNEESIKKEIENAILLKNSINLKSIK
                     KIEKNIPFIGKLIEEDKFFILNKSTFMENLEDMLEKLVHKGIIDIEFKKKVLKREKKS
                     STALDNYIALPHSVNTNGENLYLAFGILEKPVIWDTKEIRIIILMIIPDKNVDSTDLI
                     IKSYEEVLEVGRDKKMVEELAKAKSFDEFYKIITKRR"
     gene            10692..11126
                     /locus_tag="PC471_002396"
     CDS             10692..11126
                     /locus_tag="PC471_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HDF2789522.1"
                     /translation="MDPVIFIIASLITVYLLNIFLGYLQLKDFNKNYIELKRKGRVAI
                     GRKKGRISSGTIVLILINDDGVIVETRKMQGVTVLARVKSFEGLVGKNLEYIEKSDLT
                     VYNKLLKKAILDAVKQYITFEKNKCEDTNNLSTDEECRMEVV"
     gene            11155..11715
                     /gene="srlA"
                     /locus_tag="PC471_002397"
     CDS             11155..11715
                     /gene="srlA"
                     /locus_tag="PC471_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418408.1"
                     /note="catalyzes the phosphorylation of incoming sugar
                     substrates along with their translocation across the cell
                     membrane; the IIC domain forms the PTS system
                     translocation channel and contains the specific
                     substrate-binding site; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HDF2789523.1"
                     /translation="MTQFFQVLASGADSFMNLFRAGGQQFVGFVTDIVPLLVALLVTM
                     NAIINFIGTDRVERLAKKCSANIFTRYLILPVLGTFVFANPMTLSLGRFLPEKFKPSY
                     FAAASFSCHTMNGLFPHINPGELFIYLGIANGITTLGFSTADLAVRYLLVGIVANFIK
                     GVVTDYTTKFVEKQQGIALNSDIKIG"
     gene            11779..12147
                     /locus_tag="PC471_002398"
     CDS             11779..12147
                     /locus_tag="PC471_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sorbose transporter subunit IIB"
                     /protein_id="HDF2789524.1"
                     /translation="MAFNKVKIVKGSGGWGGPLIIEPTEKKNKVVYVTGGAKPETAVK
                     IAELTGCELIDGFSQGVKDDEIACVIINCGGTLRCGIYPQKKIPTINIMKTGKSGPLA
                     MFIKEDIYVSGVKPTNVEQL"
     gene            12172..12738
                     /locus_tag="PC471_002399"
     CDS             12172..12738
                     /locus_tag="PC471_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003374365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HDF2789525.1"
                     /translation="MAYDTKKKISEQSQGIVAKIGIALGNVTSTLFQAGRDTIDTLIH
                     TILPFMAFVAVLIGIINQSGIGNWVANLLTPLIGNVGGLVVLSIICSFPLLSPFLGPG
                     AVIAQIVGVLVGTEIGKGNIPPSLALPALFAINSQAACDFIPVGLGLAEAEPDTVQVG
                     VPSVLYSRFLTGAPTVLLAWLASFGLYK"
     gene            12779..13144
                     /locus_tag="PC471_002400"
     CDS             12779..13144
                     /locus_tag="PC471_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HDF2789526.1"
                     /translation="MELIYSTQINKIGDNANEFLQHNMFVTFKDNAPQELENYCYIHS
                     ENNLVKEILTDDVLSINNVDYKITSVGELVNQNLSELGHITFKFTGEKDGAIGGTLYL
                     EEKEIAPIGVGTILKIIRK"
     gene            13245..14018
                     /gene="srlD"
                     /locus_tag="PC471_002401"
     CDS             13245..14018
                     /gene="srlD"
                     /locus_tag="PC471_002401"
                     /EC_number="1.1.1.140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sorbitol-6-phosphate dehydrogenase"
                     /protein_id="HDF2789527.1"
                     /translation="MRKVALVVGGGRSLGEYLSKHLAKVGYDVAIADLNEDNAKKVAE
                     EIKTEFGCKSVGYSCNAIDERQVISTVKAIENDFDRIDLLVYNAGVAVSKKITDFGYK
                     DFKFIVDVNLNGYFLFAREVSKVMIAKGIEGRIIQINSKSGRVGSKHNSGYSAGKFAG
                     VGLTQSLALDLAEHKITVNALMLGNLLDSDMFESLIPQYAVKLGIPESEVKQVYIDKV
                     PLKRGCRFIDVANMLTFYASKEAEYCTGQSINITGGQVM"
     gene            14367..16511
                     /locus_tag="PC471_002402"
     CDS             14367..16511
                     /locus_tag="PC471_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HDF2789528.1"
                     /translation="MDIIKKLSHGCTLDCHDCCKFNVYTKGNNVVKIEGDKNHPYTKG
                     FICKKGMAHLDRLNHKDRIKTPMLKVDGVWKEISFDKAIEIMAEKLTYYKEKYTSKSV
                     MHYDQYGSGSVLKYIGDIFFNFYGGVSRHKGGPCWSAGMHAQKYDFGVAKSHAIEDML
                     NSKSIFVWGKNPAYTTIHTMQIIKKAKEKGIKIVVIDPIYTKTAQIADKYVQVNPGTD
                     GALAIAMAKIIVEDKLYDEEYINSYVIGFEEYKKYLSSLELSFLIDECGVKENDIREL
                     VDLYTNKYSSINVGYGLQKYKNGGNTIRAIDALGAITGQIGFSGGGVNYANKVYPSVI
                     NSDPYNSQSYGEDREFYVSNISKFIEESLKNTSNKVNYASDELDMTSNKVNYVSDELD
                     ITSNKTDYISNELYNLSNKSIKGNIPIKMAVITKSNMLNQLPDLVELERVFSKIEFKV
                     CFDMFITDTATLCDMFIPCTNTLESEDIIYSSMTNPYITYNERAVKPAHKLMDEYYFF
                     MELAKKMGLNDYPFVEKRTYLEKVIEPLKRFDKNLDIEKLKNNYFTIHNPVAWEDKKF
                     ETPSGKYELYSESIKNLGISPTPVYISNKYKEIEDKNISFRLLTNHHADTLFSQHFMD
                     KKSIAQAYINRRMAKKVGIEDKDIVILRSKKAKINVQINIDDGVGNYIVKMYVGWWKK
                     HGNPNSLTDTGISDFGGQVTYNESMVEIIRQN"
     gene            16599..16934
                     /gene="spoIIAA"
                     /locus_tag="PC471_002403"
     CDS             16599..16934
                     /gene="spoIIAA"
                     /locus_tag="PC471_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma F factor antagonist"
                     /protein_id="HDF2789529.1"
                     /translation="MVNYSLEHKNLYIEFMCSELDHHVANEIREEIDNLLSVNQVKNV
                     VFNFENINFMDSSGIGVIIGRYKKISNEGGRVSVINISSRVKKIFDLSGLNKIIGIYD
                     TYEEALSSL"
     gene            16948..17376
                     /locus_tag="PC471_002404"
     CDS             16948..17376
                     /locus_tag="PC471_002404"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma F factor"
                     /protein_id="HDF2789530.1"
                     /translation="MNNIMEVKFSAISENESLARVIVASFAAKLDPTVDELVDIKTAV
                     SEAVTNAIIHGYEEDSSKFVFLRCEIEGNTIKIIVEDEGYGIENVEKAMEPLYTSKPE
                     LDRSGMGFTVMKSFMDDVEVSSVKDNGTRIEMTKKINVPK"
     gene            17399..18163
                     /gene="sigF"
                     /locus_tag="PC471_002405"
     CDS             17399..18163
                     /gene="sigF"
                     /locus_tag="PC471_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigF"
                     /protein_id="HDF2789531.1"
                     /translation="MEVTVAREEKKPLLSHEETLELIEKVQNGDEEAKEILISSNLGL
                     VRSVVSRFLNIGYDREDLFQLGSIGLIKSIYKFDPKFNVKFSTYAVPMILGEIKRYLR
                     DDGMIKVSRSLKQIAIKAKMESEALTKKLGREPSIEELAKAIDVEKEDLVMAMEANFN
                     VEYLQGVIHEEEGSPICLIDKISMKGESEEEKVVDNILLKDILGRLDKRERQIIVLRY
                     FEDMTQSEIGEMLNISQVQVSRIEKKVLSKLKEYIS"
BASE COUNT         7053 a         2070 c         3166 g         5901 t
ORIGIN      
        1 cctattaaaa aatttataga tgatgaagaa atatcaaaat cttatattat aggaagagac
       61 acttctttgc caaaatctat agaaaagcaa taccaaatcc aaagagaata ggcgaagaaa
      121 aaaataaaat aaattcaaga gtaatagaaa agttttatag aataaagaac tgataactct
      181 ttatataacg aaaaatagaa ttttaagaga aaatgattta ataaatgatt taacatggtg
      241 ttttagcagg taaaaataaa tcaccagtaa tgattataga aaaattactt tatgaatctc
      301 aaaaaactta gttaagagta agagatttga taagataact aaagttgggg aaatgtgttt
      361 gatgagacaa aaaattagta aaatagtata atctaaataa ggatattttg ttgattaaac
      421 tacactaata gtaatattgt agggggaaaa gatgagagaa gaatcgaact ctttaagttt
      481 agaccatata gaaattttct ttgaactttt atctcaaagt acagaagatt atatattttt
      541 ttgggatata aataggaata aatttaaaat ttcttcagca atttttgatg aatttaactt
      601 aagtaaagag atagaatctg atttggtcaa ttgttggagt aaaattgttt atccggatga
      661 tgttcaaata tggaaagatg atatacaaga attactacat ggaaaaaaag gtgagcacaa
      721 tttagaatat aggttgatta ataaatataa agaaatagta tggatttctt gtagaggtaa
      781 agtttatgtg tcggatgacc caaaaactat atttctagtc gggagaataa agaatattgg
      841 tgaaaaaaat aaatttgatt ctatcacagg aacgtggaat agagaacaat ttgaacatag
      901 aatgaattat cttataaaag agaaaattta taaaaatggt gctatgttta ttatggatat
      961 tgataatttt aaaaatataa atgaaaaata tggacattct tatggtgaca aagtattgcg
     1021 tgcaattgct actgaagtat tagaatatct tccaaaagat gtaagattgt ataggttaga
     1081 tggagatgaa tttgcatttt tttaccctat gtgtactaaa gaaactatag agaaaatata
     1141 tgaaaagata cagatgtata caaatactca gcatgaaatt gaatcaaata agtactattg
     1201 cacagtgact gcaggcgttg ctatgtatcc agaagatgga gataattatt tagatttatt
     1261 taaacatgct gatattgcat tggatatagc aaaaattagt ggtaaaaata gaattaagtt
     1321 tttttcacaa gagctctatg aaaataaact aaaggtaatt tcaatgcaac aaaaactaag
     1381 agagtgcgtt gaaaataatt ttaatgattt tgagttgttt tttcaacccc aggtaaatgc
     1441 tgttacaaaa gaggtaatag gggcagaggt acttttaaga tggcattcga gtacttatgg
     1501 agaagtatct cctgttgaat ttataccaat actagaacag agtaatttaa ttataccagt
     1561 tgggaagtgg attataaaag aagctgtaaa acagtgtaaa gaatggcata aaataaatcc
     1621 agactttaaa atatctgtaa atgtatcgta tattcagtta aaggaagatt tttttagaga
     1681 ttttatagta gaatgtttag tagagtatca attaagacct gaatttttaa ttttggaatt
     1741 aacagagaat tgttggatac cagatataaa tttactaaat gataaattta ttagtttaaa
     1801 gggtatagga gtatatattg caatagatga ctttggtaca ggatattcat ctttaaatta
     1861 ccttaaagaa ctgtctgtta atataataaa aattgaaaga agttttgtta agaatattac
     1921 atataatagt tatgaatata cttttcttga atatattatt aaattagctc atattataaa
     1981 tttaaaagtt tgtgttgagg gaatagagtc ttatgaagag tatgatatag taaaaagctt
     2041 aggtgtagat attattcagg gttttttatt tggaagacct gtaagtgcct ctgaatttta
     2101 taaatttcag ctatcaaagt gatgtatata aaatgagata tatagtatat aaaaataggg
     2161 caaagtggaa gttttgaagt ttattaaaaa tttacacttt accctatttt gttatgtctt
     2221 ctataaagga ttaatatact acactaacta ttatatactt tcactccaaa tttagagatt
     2281 atttcactag ctttttggac atcttcctta gtggcatcag gaacatcttt tagttcataa
     2341 tcgataccta aactttccca tttgtgtatt cccatagaat ggtatggtag caattcaaat
     2401 ctttctacat ttttcaatgt agctacaaat tgacctagtt tttctaaatt atctatatca
     2461 tcagtaatgc caggaactaa aacatgtctt atccatacag gaatatttct tgattctaag
     2521 tgtctagcaa gttttaatgc cttatccata ccaacaccag tcaaatcaat agatttttcc
     2581 tcaaccatat gttttaaatc aagtaaaacc aaatcaatat tatctaaaac tgggtctata
     2641 gtttctatat ctacaaatcc agatgtatcc aagcaagtgt gtatatcatt ttcttttgct
     2701 tttttaaata attcacttaa aaattctggt tgaagagtgg attcaccacc agaggcagtt
     2761 atacctccac cagaaaactt cataaaagaa gtatacttaa gagcatccgt tattatttca
     2821 tcaactgtgt attcttttcc tgatttaata tcccatgtat ccctgttatg acagtatttg
     2881 catctaagag gacacccctg aaaaaataat atatatctta ttcctggtcc atctacagta
     2941 ccaaacgttt ctatagaatg tatctttccc ttaatcatag agttcacctc acaatatatt
     3001 ttaataacct tatcaatagt tataatattt ctctaaaaaa ggccatctta taccagaaat
     3061 aattcaaata taagatagcc ttttattttt attttttaca cagtagtaat cttagttaaa
     3121 gtataaaaat ttaaagacta agccatttta ccgtggaatg ttctgtttat aacatctaat
     3181 tgttgctctt tagttaattt tatgaagtta actgcatatc cagatactct tatagttaat
     3241 tgtggatatt cttctggatg ttccatagca tcttctaaag tagctctatc aaatacattt
     3301 acatttaagt gatgagcatt ttgagcgaag tatccatcca tcattgcaga aagattgttt
     3361 attctttcag tcatatcttt tcctaaagca ccaggtacta tagagaaagt attagatata
     3421 ccatcttgag catgttcata tggtaattta gcaactgaag ataatgaagc taaagcacca
     3481 ttagtatctc taccatgcat tgggttagca ccaggagcaa atggagcacc agctcttcta
     3541 ccatctggtg tattaccagt tttcttacca taaacaacat ttgaagttat tgtaagtata
     3601 gattgagttg gataagaatt tctataagtt ttattctttc ttattttatt cataaagctt
     3661 tcaactaact ctacagctat actatcaact ctatcatcat tatttccgta ttttggataa
     3721 tctccttcta tttcaaaatc aacagctatt ccttgttcat ttctaatagt tttaacctta
     3781 gcatgtttta tagcagataa tgagtctgca caaactgata acccagccat accacaagcc
     3841 atagttctaa atatatctct atcatgtaat gccatttcta atgcttcata agaatattta
     3901 tcatgcatat aatgtattac atttaaagta tttacatata atgttgctaa ccaatcagtg
     3961 aatggttcaa atttactcat aacttcatca taatctaagt actcagaagt tataggttca
     4021 aatcttggtc caacttgaac accagatttc tcatctacac caccatttat agcgtataat
     4081 aaagttttag ctaagttaac tctagctcca aagaactgca tttgtttacc aattctcatt
     4141 gcagatacac aacaagctat accatagtca tctccccagt atgctctcat taaatcatca
     4201 ttttcatact gtactgaact tgtatctata gatactttag aacagaaatc tttgaatcct
     4261 tgaggtaatt tagttgacca tagaactgtt aagtttggtt ctggagaagg tcctaatgta
     4321 tatagagtat ttaacattct aaatgagttt ttagtaacta atgttctacc atctattccc
     4381 atacctccta tacactcagt tacccaagtt gggtctccag agaataggtt attgtaatca
     4441 ggagttctta ggaacttaac cattcttaat ttcataacaa aatggtccat atactcttgt
     4501 aattcttctt ctgttacgat tccagctttt aaatctcttt caaagtatat atctaagaaa
     4561 gtagatgttc tacctaaaga cattgcagca ccattttggt ctttaacagc acctaaataa
     4621 ccaaagtata accattgtac agcttctttt gagttagttg ctggttttga tatatcaaat
     4681 ccataagatt cagccatttt tttaagttca tttaaagctg atatttgctc agttatttct
     4741 tctcttaatc tgataacttc ctcatccatg caagaaactt ctaatgattt cttttgctca
     4801 tttttatctt ctattaaagc atcaacaccg tatagagcaa ctcttctgta gtcaccaatt
     4861 attctacctc taccataagc atctggaaga cctgttatta taccagattt tctagcagct
     4921 ctcatttctg gagtataaac atcaaaaact ccttggttgt gtgtttttct gtatttagta
     4981 aatatatctt ttatttctgg gtctaattca tatccaaaag cttcacatga attttcaacc
     5041 atctttatac caccatatgg catgacagct ctttttaaag gggcatccgt ttgtaaccca
     5101 actattgttt ctaattcttt atctaaatat cctggagcat atgcagctat tccagaaaca
     5161 gtttttgtat caacatctaa agtaccacca ttttctcttt cttttttaaa taggaccatt
     5221 gcttcgtccc ataatttttt agtattttca gtggcaccag ctaagaaaga gtcatttccc
     5281 tcatatggag aatagtttaa ttgtataaac tctctaacat ttatttcttt agtccatctt
     5341 cctgttttaa atccttgcca tgcattcatt aagtatttcc tccttagatt taaatgataa
     5401 ataatactat taaaattaat ttttatatac ttaatttatt ttatatattt ttttaaaaat
     5461 atattttcta atataataat agcatattat tcgtatcctg tatacataaa aaataaaaaa
     5521 ttgtcgcttt aaagtataaa acagattata tgttaataat gtggtgaaat tatacccata
     5581 tataaataaa ataaaacaaa taaatagcta attaaggaga aaatattttt tattttttgt
     5641 ttaggataaa taaattttat tatggaaaaa agaaaaagca tacatcttca caaactgaat
     5701 atttatttaa tagggggagg tgatagattg tttatcacag tggtattatt tttagtaggt
     5761 ttcctattaa ttactaaagg cgcagatatc tttataaatt gtactgtaga gataggtaaa
     5821 aaaacgaata tatcagaaat tatacttgga gctactatag ttagttttgc aacaacatta
     5881 cctgagttta cagtgtctct acttgcttct attgatggtc atactactat gagtttagga
     5941 aatgcagttg gttctataat atgtaataca gggttagcat tagggttggt tgtttttata
     6001 agtccattta atgtagataa aaaaatgttt ttttctaagt ctttactatt aatagtatcc
     6061 gttatagttt taattttatt gagtttagat ggagttataa caagaggtga ttcattatta
     6121 cttataataa tattaatctt ttatatggtt aataattata gaagtgtcgt tggaaaatct
     6181 gatactaaaa atagaaatat taaaaataac actattaaag acatatctac taaaaaaggg
     6241 aaaaattata gaggtttttc cgtattagaa attgtaaaaa tactattact ttttgctaca
     6301 ggattgataa tgatgataat aggttcacaa atccttatag agagtggagt aataatagct
     6361 agttttttaa atatacctca aggtatagta agtttaacta taatagcact tggaacttct
     6421 ctgccagaga tagtttcgtc gattactgct ataagaaaaa atcatcatga aatatcagta
     6481 ggaaatattt tgggtgcaaa catattgaat atagtatcag taatagctgt atcagcaata
     6541 ccaaataaca taccaatact atctcaaaat agacaattgg atataccatt catgatatta
     6601 ttattattaa tagtaataat accaacactt aaatctaata aacttagtag aatacaaggt
     6661 atattgatgt tattcacata ttttctgtac atatcaattt tgtatttcat gtatattatt
     6721 taactatact gtttaaaata ttaattgcag aatatcctaa aaaagtatat aatggatagg
     6781 ttaagattaa ttgataacgg agaggaataa tatatgaata taacaaagtt tgaagattta
     6841 ccaataagtg agggaataaa aaaagctata gctgaaatgg ggtttgaaga accatctcct
     6901 atacaagcac agtctatacc tgcgattttg tcaggtaaag acgttatagg acaagctcaa
     6961 actggtacgg gaaaaacagc tgcttttagt atccctatac tagaaacaat agacccaaat
     7021 aatagaagtt tacaagctgt agtgctttgt ccaacaagag aactggcaat acaagtatca
     7081 acagaaataa gaaagttagc taagtattcg cacggaataa agacattacc tatatatggt
     7141 ggtcagccaa tagatagaca aataaaatct ctaaagagtg gtgtgcaagt tgtaataggt
     7201 acaccaggac gtacaataga ccatatcaat cgtaaaacat taaagatgga taatgtaaaa
     7261 atgattatat tagatgaagc agatgaaatg ctagatatgg gatttagaga agatatagaa
     7321 atgattttaa gtaagattcc agaagaaaga caaacaactt tcttttcagc tactatgcca
     7381 agaggaattt tggaattaac taagagatat caaaaagacc cagagcatat aaaagttgtt
     7441 agaaaagaac ttacagtttc aaatacaaag caatactata tagaaactag gtcatcaaat
     7501 aaattagaag tattatgtag attagtagat gtatatgacc ctaaactatc tgttgttttc
     7561 tgtaatacta agagaaaagc agacgaattg gtaggagatt tacaagcaag aggatatttt
     7621 gcagatgcat tacatggtga cttaaaacag actcaaagag atatagtaat ggataagttt
     7681 agaaatggta caatagatat acttgttgct actgatgttg cagctagagg tatagatgtt
     7741 gatgatgttg aatgtgtatt caactatgac ttaccacaag atgaagaata ttacgttcat
     7801 agaattggta gaactggtcg tgctggtaga gaaggtatgt catttacttt tgtatttgga
     7861 aaagaaatga gaaagatgaa agatatagaa agatatacta aatctaaact aataaagcat
     7921 aatataccaa caataactga tgttgaagag aaaaaagtag gtactttctt tgcacaagtt
     7981 aaacaaacta tagaagaagg tcacttaact aaacaattac aatggttaga aggtttctgt
     8041 aatgatgaag attatgcaat ggttgatata gctgctgcac ttgtaaaact atctctaggt
     8101 gaagaaatga aagaagaaat aatagaagaa aaacctagaa gagagcgtgg agatagaaaa
     8161 ggtggtactg gagctaaaga tggtatgatt agattattta tcaatattgg tagaaatcaa
     8221 agagtacaag caaaagatat agttggtgcc atagctggag aagttggaat accaggtaaa
     8281 gtagtaggta ctatagatat atatgataag tatacttttg ttgaaatacc taaaaaagat
     8341 gcaaaaacag taattgaaaa gatgaaagat attaagataa aaggtaataa gattaatata
     8401 gaaaaagcaa ataagaagaa aaaataacgt atagtataca aaactataat tagaaaatat
     8461 ataaaataat atattggata tttaaaaagc ctgatgtgtt ttcaaaacag atagtagata
     8521 atctgtaaga gttaacagca taaggctttt cttgttttat atgatgttat aattttccag
     8581 attgttgtga aaaatgtata ttagttcctt tttttcaaat ctgatttgaa aaagagaata
     8641 ttaattatct atatagttta gattataata aaaaatgtaa gataacaaag gggagaaaaa
     8701 gatgtcaatg acttatatgg accctagggt ttttttgatt ttagaaatgg tgttagaaga
     8761 agacacaaat actctagaac aattttcaaa tgttttgggt gttagtacta ggactatcag
     8821 aaactatata aaccagatta atcgagaatt aaaaggtggt attgctaaaa tagttaaaaa
     8881 tgaaaaaggt acatatagta taagaattga ggatgaaagt aaattaattg agattgtaaa
     8941 ttttaataga ggaaagctat caaattttat aaatttaaat tcacctgatg aaagaataaa
     9001 ttatgttctt gatattttaa ctatgaccaa caatccaatt accatagatg atttggctga
     9061 agagatatgt ataggaagaa caacattaat aaaggattta aaaaaagcag acagaatttt
     9121 agctgaatat gatttagaat taaaaaggaa gccaaatata ggtatgattt tagttggtga
     9181 agaaatggat ataagattat taatccttga tagattgtat gaaaattata ttgatgtctt
     9241 ggaaaacgtt aatcagataa atttagttaa agattatgat attgaatgtc ttagagatga
     9301 attaaaaaag ttatttaaaa aagaagaact ctacataaca gaacaggtct tgagagatgt
     9361 tgaaagatat ataatcatgt ctgtcttaag aaatctaaat gggtacaaat ttgaaaagat
     9421 agataagaga tttgaagtga taagatgttc agatgaatat actttaggat taaagctcaa
     9481 agctttattg gaaaagatat ttaatatagt tttaaatgaa aaagaaacca tatttttgac
     9541 aatgccatta ataggtagaa aagctcctag tacaaaatta gcacttagca gtataaagat
     9601 aaatgatagc gtaaaggaag ttgtagatga agtaactagt tttttattag agacctcagg
     9661 gattgatttt aaagaagata aaaagcttat agaaaatttg gaatatcact tgtattttgc
     9721 acttaataga atgagattca acataagggt aaaaaatcct cttttacaag aaataaagga
     9781 aagatataca tttccatata gtgttgcaaa gatagctgca atgataattg aggaaaagtt
     9841 tgacttgaaa atatgtgacc acgaaatagg atatatagca ttacattttg gaagttattt
     9901 tgaaaaaaat tcaagaaaag ttctaaatgt aaatagggta gctgttgttt gtggaacagg
     9961 tcttgggact gcacaactta ttagaataaa gataggaaaa atacttggag aaagtgtaga
    10021 agtaaatact ttttctgata tagaagctaa agctaattta gatttattaa agtattatga
    10081 tttagtattt acaacagtag atttcaaaac agacttagat gttccaattt ttagagtaaa
    10141 tgcgttattt aatgaagaaa gtataaagaa agagatagaa aatgcaatac ttttgaaaaa
    10201 tagtataaat cttaagtcta taaaaaagat tgaaaaaaat atccctttta taggtaaatt
    10261 aattgaagag gataagttct ttatattgaa taagagtacg tttatggaaa atttagaaga
    10321 tatgttagaa aaattagttc ataaaggaat tattgatata gagttcaaaa aaaaggtatt
    10381 aaaaagagaa aagaaatctt caacagcatt agataattat atagcattgc ctcactcagt
    10441 taatactaat ggggaaaatc tttatctggc atttggaata ttagaaaaac cagtgatttg
    10501 ggatacaaag gaaattagaa taataatatt gatgataatt ccagataaga atgtagatag
    10561 tacagacctg ataataaaaa gttatgaaga agtacttgaa gtagggagag ataaaaagat
    10621 ggtagaagaa cttgctaagg caaaatcatt tgatgaattt tataaaataa ttaccaagag
    10681 gaggtaataa tatggaccca gtaattttta ttatagcatc acttattaca gtgtatttat
    10741 taaatatttt tcttggatac ttacagctta aagattttaa taaaaattat attgaactaa
    10801 aaagaaaagg aagagtggca attggcagaa aaaaaggaag aatttcatcg ggtacaattg
    10861 tgttgatact tataaatgat gatggtgtga ttgtcgagac tagaaaaatg caaggtgtta
    10921 cagtattggc aagagtaaag tcatttgaag gtttggtagg aaagaactta gagtatatag
    10981 aaaaatcaga tttaactgtt tacaataaac tacttaaaaa agcaatacta gatgcagtca
    11041 aacagtacat aacatttgag aaaaataaat gtgaagatac aaataatctg agtactgatg
    11101 aagagtgtag gatggaagta gtttaaaaaa atatatttat aaggaggagt taatatgacg
    11161 caattttttc aagtattagc aagtggtgca gatagtttta tgaatttatt tagggcaggt
    11221 gggcaacaat ttgtaggttt tgtaacagat atagtaccac ttttagtagc attattagtt
    11281 acaatgaatg caattatcaa ctttattggt acagatagag tcgagagact tgcaaaaaaa
    11341 tgttctgcaa atatatttac tagatatttg atattaccag tccttggtac atttgtattt
    11401 gcaaacccaa tgacactatc attaggaaga ttcttgccag aaaagtttaa acctagttac
    11461 tttgcagcag caagcttttc atgccataca atgaatggat tattcccaca tatcaatcca
    11521 ggtgaactat ttatatatct aggaattgca aatggtataa caactttggg attctcaaca
    11581 gcagatttag cagttagata tttgttagtg ggtatagttg caaactttat taaaggtgta
    11641 gttactgact atacaacaaa atttgtagag aaacaacaag gaatagcatt aaattcagat
    11701 ataaagattg gataattact ttaaggtata aataaatttt tcaaataaaa agatagtgta
    11761 tagaggagga taaattatat ggcatttaac aaagttaaaa tagtaaaagg ttctggagga
    11821 tggggtggac cattgataat agaaccaaca gaaaagaaga ataaggtagt atatgtgact
    11881 gggggagcaa agccagaaac tgcagtaaaa atagctgaac ttacaggatg tgagttaata
    11941 gatggatttt cacagggtgt taaagatgat gaaatcgcat gtgtaataat aaattgtggt
    12001 ggaacattga gatgtggtat atatcctcaa aagaaaatac cgactataaa tataatgaaa
    12061 acaggaaaaa gtggaccatt agcaatgttt ataaaagaag atatatatgt ttcaggggta
    12121 aaaccaacaa atgtagagca gttataattg aggtagtagg agggaagaaa tatggcttat
    12181 gatactaaaa agaagataag tgagcagagt caaggaattg ttgcaaaaat aggtatagca
    12241 ctaggaaatg tgacaagtac tctttttcaa gctggtaggg atactataga tacattaata
    12301 catactatac tgccttttat ggctttcgtt gcagtcctaa taggtataat aaatcaatca
    12361 ggtataggaa attgggtagc caatttactt actccattaa tcggaaatgt tggaggttta
    12421 gttgtcttat caataatatg ttcatttccg ttgttgtctc cgtttcttgg accaggagct
    12481 gttatagctc agatagtggg agtattggtt ggtacagaaa taggaaaagg aaatattcca
    12541 ccaagtttag cattaccagc attgtttgca ataaactcac aagcagcttg tgactttata
    12601 ccagtaggtc ttggtcttgc agaagctgaa cctgatacag ttcaagtagg agttccgtca
    12661 gttttatatt ctagattttt aacaggtgca ccaactgttc tattagcatg gttagcaagc
    12721 tttgggttat ataaatagaa gaatacttta taaataatta gagagaggtt cagttgatat
    12781 ggaattaata tacagtactc aaataaataa aataggagat aatgcaaatg aatttttaca
    12841 acataatatg tttgtaactt tcaaagataa tgctcctcaa gagttagaaa attactgtta
    12901 tatacattct gaaaataatt tagtaaaaga aatacttacg gatgatgtgc tatcaataaa
    12961 taatgttgat tacaaaatta cttctgttgg tgagttagta aaccaaaatc tttcagaatt
    13021 aggtcatata acatttaagt ttacaggtga aaaagatgga gcaattggag gaaccttata
    13081 tcttgaagaa aaagaaatag cacctattgg cgtaggtact atactaaaaa taattagaaa
    13141 ataaaattta aaacgaatct aatcatatta taagtaaatt atagataaaa gtcaatttaa
    13201 ctaatcaagt taacttttaa tgtgataaaa aaggggaaat gaaaatgaga aaagtggctt
    13261 tagtcgttgg aggaggaaga agtttaggtg aatatttatc taaacattta gctaaagtag
    13321 gttatgatgt tgctattgca gatttaaatg aagacaacgc aaaaaaagta gctgaagaaa
    13381 taaaaactga atttggttgt aaatctgtag gatatagttg taatgccata gatgaaagac
    13441 aggttataag tactgtcaaa gctattgaaa atgattttga tagaatagat ttattagtgt
    13501 acaatgcagg agtggctgta agtaaaaaaa taactgattt tggatacaaa gattttaaat
    13561 ttattgtaga cgtaaattta aatgggtact ttctatttgc aagagaagta tctaaagtca
    13621 tgatagcaaa aggtattgag ggtagaataa tacagataaa ttcaaaatca ggtagagtgg
    13681 gaagcaagca taattcagga tattcagcag gtaaatttgc tggagtagga cttactcaaa
    13741 gtttagcgct agatttggca gaacataaga taactgtaaa tgcactgatg ctcggtaatt
    13801 tacttgattc tgatatgttt gaaagtttaa ttcctcaata tgcagtaaaa ttgggaatac
    13861 ctgaatcaga agtcaagcaa gtttatatag ataaagtacc tttaaaaaga gggtgtagat
    13921 ttatagatgt tgcaaatatg cttacatttt atgcttcaaa ggaagctgaa tactgcactg
    13981 gtcaatctat aaatattact ggtggacaag ttatgtaagg tttttatgga gtgtctcaag
    14041 atgaactctt tagttcatga ggcacttttt tgtataaaat taaatatatt ttcagcattt
    14101 aaacaatgaa aaagaggttt atctttattt tgagatagcc tcatttttgt aattaaaaaa
    14161 catagtgttt aagtaaacat ataagattgt tagtttttta aaagagaatt ttttaataat
    14221 attatattta tgaagtattt ttaaacttat tttgataaaa tatagctatg ttttatatac
    14281 tgtagttttg aacatagaaa gtttaataaa ttatagtata tatgttaatt tttaataagg
    14341 taagaaagta gaaagaagga tattttatgg atataataaa aaaattaagt catgggtgta
    14401 cattagattg tcatgactgt tgtaaattca atgtttatac taaaggaaat aatgtagtaa
    14461 aaattgaagg agataaaaat catccttata caaaaggttt tatatgcaaa aaagggatgg
    14521 cccatttaga tagattaaat cataaagata gaataaagac tcctatgctt aaagttgatg
    14581 gagtatggaa agagatttct tttgataaag ctatagaaat aatggcagaa aagcttacat
    14641 actataaaga aaagtataca tcaaagtctg tcatgcatta tgaccaatat ggaagtgggt
    14701 ctgtgctaaa atacatagga gatatatttt ttaattttta tggaggtgtt agcagacata
    14761 aaggtggtcc ctgttggagt gcagggatgc atgctcaaaa atatgatttt ggagtagcta
    14821 agagtcatgc tatagaagat atgttaaata gtaaaagtat atttgtgtgg ggaaaaaatc
    14881 cagcatatac tacaattcat actatgcaaa taattaaaaa agctaaagaa aaagggataa
    14941 agatagtagt gattgacccc atatatacaa aaacagctca aatagcagat aagtatgtac
    15001 aagtaaatcc tggaacagat ggagctttag ctatagctat ggcaaaaatt atagtagaag
    15061 ataaattata tgatgaagaa tatataaact cgtatgttat tggttttgaa gaatataaaa
    15121 aatatttatc ttcattggaa ttaagttttt taattgatga atgtggtgtt aaagagaatg
    15181 atataagaga gttagtggat ttatatacta acaaatattc aagtataaat gttggatatg
    15241 ggcttcaaaa atataaaaat ggtggaaaca caataagagc aatagatgct ttaggtgcta
    15301 taactggtca aataggattt agtggtggag gggtaaacta tgctaataaa gtttatccga
    15361 gtgttataaa ttctgaccct tacaatagcc aatcatatgg tgaagatagg gagttttatg
    15421 taagtaatat cagtaaattt atagaagaat ctttaaaaaa tacatcaaat aaagtaaatt
    15481 atgcatcaga tgaacttgat atgacatcaa ataaagtaaa ttatgtatca gatgaacttg
    15541 atataacatc aaataaaaca gattatatat caaatgaatt gtataattta tctaataagt
    15601 caatcaaagg taatattcct ataaagatgg ctgtaataac taaaagtaat atgctgaatc
    15661 aactgccaga tttagtagaa ttggagaggg tgttttcaaa aattgaattt aaagtatgtt
    15721 ttgatatgtt tataactgat acagctactt tatgtgacat gtttataccg tgtacaaata
    15781 cactagagag tgaagatata atttatagct caatgaccaa tccatatata acctataatg
    15841 aaagagctgt aaagccagca cataaattga tggatgaata ttacttcttt atggagctag
    15901 ctaaaaagat gggattaaat gattatcctt ttgtagaaaa gagaacttat ttagaaaaag
    15961 tgattgaacc acttaagcgt tttgacaaaa atctggatat agaaaaactt aaaaacaatt
    16021 actttactat acataaccct gttgcttggg aagataagaa gtttgagact ccatctggaa
    16081 aatacgagct ttattcagaa agtataaaaa acttgggaat aagtccaaca cctgtatata
    16141 taagcaataa atataaagaa atagaagata aaaatatttc ttttagatta ttaactaatc
    16201 atcatgcaga tactttattt agtcagcatt ttatggacaa aaaatctata gctcaagcat
    16261 atataaatcg gagaatggct aaaaaagtag gtattgagga caaagatatt gtgatattga
    16321 ggtcaaaaaa agcaaaaata aatgtacaaa taaatataga tgatggagta ggtaattata
    16381 tagtaaaaat gtatgttggt tggtggaaga aacatggaaa tcctaatagt ttaacagaca
    16441 ctggtatatc agattttggg ggtcaggtaa catataatga aagtatggta gaaataatac
    16501 gacaaaacta gttaaatttc attgaaggaa taaaaatata attatagaat tgattaaaag
    16561 ttactatttt tacaaaacta ttgaaggagg gatttttgat ggtaaattat tccttagaac
    16621 ataagaatct atacatagaa tttatgtgct cagaactaga ccaccatgta gccaatgaaa
    16681 taagggaaga aatagataac ttattaagtg taaatcaagt aaaaaatgtt gtatttaatt
    16741 ttgaaaatat taattttatg gattcttcag gtataggcgt catcataggt agatataaaa
    16801 agatttctaa tgaaggggga cgagtttcag taattaatat aagttcacga gttaagaaga
    16861 tttttgattt atctgggtta aataaaatta ttggcattta tgatacgtat gaagaagctc
    16921 taagttcttt gtagggagga gtatgtgatg aataatatta tggaagttaa attttcggca
    16981 atatctgaaa atgaaagttt agcaagagtt atagtagcat cctttgcagc taagttagac
    17041 ccaacagtag atgagttagt agatataaaa actgctgtct ctgaggctgt tacgaatgct
    17101 ataatacatg gatatgaaga agacagttct aagtttgttt tcttaagatg tgagatagaa
    17161 ggaaatacta taaagattat agttgaagat gaagggtatg gaatagaaaa tgtggagaag
    17221 gcaatggaac cactttatac atcaaaacca gaacttgata gatcaggaat gggattcaca
    17281 gtaatgaaaa gctttatgga cgatgtagag gtaagttctg taaaagataa tgggactaga
    17341 attgaaatga ctaaaaaaat aaatgtgcct aaataaagtc caaaagtgag gttttattat
    17401 ggaagtaact gttgccagag aagaaaaaaa acctcttctt agtcatgaag agactctaga
    17461 acttattgaa aaagttcaaa atggagatga agaggcaaaa gaaatattaa tatcaagcaa
    17521 tttaggtcta gttaggagcg ttgtatctag atttttaaat ataggatatg atagagaaga
    17581 tttatttcag ttaggttcta taggcttaat aaaatcaata tataaatttg acccaaaatt
    17641 taatgtaaaa ttctctactt atgcagtacc aatgatttta ggtgaaataa agagatactt
    17701 aagagatgat ggtatgataa aagtatcaag gtcgttaaag caaatagcta taaaggcaaa
    17761 gatggaaagt gaagctttaa ctaaaaaatt aggtagagag ccgagtatag aagaattagc
    17821 taaagcaata gatgtagaaa aagaagattt agtaatggct atggaagcta atttcaatgt
    17881 tgagtatttg caaggagtta ttcatgaaga ggaaggttct ccaatatgcc ttattgataa
    17941 aataagcatg aaaggagaaa gtgaagaaga aaaagtagtg gataacatat tgttaaaaga
    18001 catacttgga agactggata agagagagcg tcaaataata gttctcagat actttgaaga
    18061 catgactcaa agtgaaatag gagagatgct caatatatca caagttcaag tatctaggat
    18121 agagaagaaa gtattgtcta agttaaaaga atatatatcg tgataatact tatgatatgt
    18181 agaataataa
//
LOCUS       DAMJYR010000073        18019 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.073, whole genome shotgun sequence.
ACCESSION   DAMJYR010000073 DAMJYR010000000
VERSION     DAMJYR010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 18019)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18019)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18019
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.073"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            505..1365
                     /locus_tag="PC471_002406"
     CDS             505..1365
                     /locus_tag="PC471_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDF2789532.1"
                     /translation="MKIIQILNNNVALVKRGKNEIIVFVKGIGFKKRVGQVINENEIE
                     RTYILDSYDMLEHFSYLLQNSDSNNIVLINEIIEYGEKTLNIKASDYLSLTLLDHLEF
                     LLKRCKKNQFIKSPLVWNVKRFYPKHFEIGMHALKLIGDSKGINLPDDEAVSIALHFV
                     NMEVNKESHDSTIVELRTLADIVSIIKYHFNVELDETSTNYMRFTTHLQYFIQRIMSE
                     EASIDEDNSIDLYKQVSQLYPKSFGSVQKIRKYIKSQFNVDISISEETYLMLHINRVT
                     QRLEAKSDEI"
     gene            1355..3226
                     /locus_tag="PC471_002407"
     CDS             1355..3226
                     /locus_tag="PC471_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDF2789533.1"
                     /translation="MKYKDLNENIIKLIGGKENIKSVAHCVTRLRFTLNDRSIAQTEE
                     IKKLKGVIDVVSNDVAYQIIIGTHVVDVYNEFMSMIGLSSSESSIKSERKKGIRGIKG
                     IFNSIFVVVSETMTSVIEVLLAAGILASILALLNMSGILQSDSPTYQILDTLRSATFH
                     FLPVLIAIASAKRLNVNPYIAVVLAVTLLSSSIDGVEGLSLFGINLQSTTYANSFIPI
                     LLGVWAMGIIIEGLKKILPKALHYFLVPVLSLIITLSVTLTVFGPIGMWIGDGLNYAC
                     MFLIDTLGNWSVVALYAALQPLLIVFGAANFTMPISLNSVTTLGYDPIFMGAATISDI
                     AVCGAMFGYFLKTRNKEQKQLFGTVSFSALMGVTEPSVFGVFMKYRRPFVAVMIGGGI
                     GGLIAGLAQVKTYGMVWGLAALPTYLVGGEVSNFTFMIISVVVSFVAATAASYLLGIP
                     SDEKEEVKGDNEKELELIINENNSRTISIGNVAKGQVIALGEVNDKAFSSGALGKGVG
                     VIPSESKSTVISPVDGTITIVFPTKHAYGIVTDEGLEILIHIGIDTVNLDGKFFESLV
                     TQNQRVKKGDALAVFESDKIIEEGFDSTIITVVTNTSDYLDVISSNDESEELLTVIV"
     gene            3246..4700
                     /locus_tag="PC471_002408"
     CDS             3246..4700
                     /locus_tag="PC471_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HDF2789534.1"
                     /translation="MNIKDTFFWGGSIAAHQCEGSWDSDNKGPAIMDFVTKGSYEMPR
                     VITDKIEEKLDYPSHNGIDFYNRYKEDIALFKEMGFSALRISIDWSRIFPNGDDENPN
                     ELGIKYYEGLIDTLIENNIEPIVTLYHFELPMNLVHKYGSWNNRKLIDLYLKYSETVI
                     RRFDDKVKYWVTFNEMNHIDPQTEHSDIFTYMIAGIKYSEIENKVQTLANVGYNMTLA
                     GVKVAKLAREINPNNKVGCVFGLNPIYPYNCNPSNVLNAFLDNDRDYYQIDAMCNGKF
                     PQYKLKEYEKNNITIEVTDEDKKAFKEGKLDFIGLNYYFSSVSTPKENLSGDKSLFGG
                     IQNPYLEQSKWGWAIDPIGLRFTLNYIYRKYQLPILITENGLGAYDKIEEDGTINDDY
                     RIEYLGKHLSQMKSAIEEDNVECFGYLMWGPIDLVSATTGQMSKRYGFIYVDLDDEGK
                     GSFKRYKKKSFDWYKEVIKSNGEVLDFSTDIVVG"
     gene            5725..6933
                     /locus_tag="PC471_002409"
     CDS             5725..6933
                     /locus_tag="PC471_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2789535.1"
                     /translation="MENFWNPLTCFTVCVMIYAFSEFLSKKTKGAISTLLFVCIIFLL
                     GFWTNILPKDITGASGILAAMSNFGIALLITNLGTLINLEDLCHEWKTVVIALVGIVG
                     IGVGCLTVGSMLFGREYALIAAPPIAGSTVAGIIVTTVAEAANRPELAAFVILVLSFQ
                     KFFGIPITTYCINKELKFKRDNGDFLKDESKSNFKLPSMRIFKEDLGKKSSTLVYLAK
                     LSFVAMIGNFVGLATLIPGFTPANYYLNPNIACLLVGLIFTRIGFLEKGILDKSQSNG
                     IIMFGCMLMLPGGLAKVTPTALFGMIVPTIGILVVGSVFIILFTAIVGKLLGYSPRMS
                     AAIGVTCMLAYPATQIISNEGIDAMEGTEEEKQRATDYLLPKMIIGGFVTVTIASVAF
                     ASIIGPIIFG"
     gene            6964..8343
                     /locus_tag="PC471_002410"
     CDS             6964..8343
                     /locus_tag="PC471_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HDF2789536.1"
                     /translation="MIGSISINEIDKKRFKLDDLSKKIWENPEKAFKEFKACENTANF
                     LRSEGFDVEVGVGGLATAIRASFGSGKPVIGFMGEFDALPGLNQKVSTKQEAFELGAY
                     GHGCGHNLLCTAHVGAVVGLKREMIENNLSGTIVFYACPGEEQLTGKGFMARGGAFEG
                     LDLAINFHPNKISEATIGTSTAVNSVKFHFKGKTAHAGSDPQNGRSALDAVELTNVGA
                     NYLREHVTSDVRIHYTITDGGVAPNIVPDKASVWYYTRALSREAVESTYERLVKVAKG
                     AAMMTETEVEVEFLGGCYNTLNNHVLANLVSECMNEIKQEPWTREELDFAAELDRQSP
                     QQYKAMISKYNLPEGTHLYYGGGNVTNFNSYGSTDIGDVMHIVPTAYFFTGCTNLGAP
                     GHSWQFTSCAGSSIGEKGMIYASKIMAMFGAKILNNPEIAKKAKEEFDKSMNGKTYKC
                     PIPDDIPTP"
     gene            8449..9639
                     /locus_tag="PC471_002411"
     CDS             8449..9639
                     /locus_tag="PC471_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HDF2789537.1"
                     /translation="MKNFFKEAQSIEGEIIRWRRYLHENPEIGCNLPKTVTFVTSTLE
                     KMGYSPKLTSNNGIVAVLDSENGGKTLLLRADMDALPIREETDLPFKSKNKDASHSCG
                     HDNHTAMLLGAAKILMDNKHLLKGRVKFVFEPDEETTRGAKSMIDDGILENPQVDAAM
                     AFHSMVGKFLNTGQVAYSRGPAMASADIFEITVEGKATHGASPEFGVDPINIMSHIQI
                     ALNTILSREKPQKEPVVLTIGMFNAGNVPNAIPNKASMSGTLRTFDQFVREKLKNRII
                     EISTGIAKSFGGNAKVEFKMGTPAVINDLDVGDEIVSYVKEVIGNENVIDFPSAMGSE
                     DFSEVLLRVPGVFLWIGMGSISQGYNFGMHNPKAIFNEEGLVYGSALFAHCAVKWLEN
                     NFSR"
     gene            complement(9767..11095)
                     /locus_tag="PC471_002412"
     CDS             complement(9767..11095)
                     /locus_tag="PC471_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2789538.1"
                     /translation="MMKIAVITTEFLKEFVDNSIKKLNINAEIEIYIYRDFSHVGDLY
                     LEIEDRFDGFAVSGPIPKKAITKKAGTIKKPLVDFGTDLQSYYDMFLKLIFKYNTLDF
                     SRAYFDLTDWADNPKDISYYLSNGTFGDLMDSIDENASKMSLSDIAKLEKKIAKKHIN
                     LWNEGKIDFSTTRFSSIVPMLQNAGVNFHFIYPDIDLLKNTFNNLIKDIHLHRMKGNQ
                     PSVIYITVKDTNYDDSTLDQVYKILNSIKKDKLTDFIIEKNSNSFKIFTKCETIKSLT
                     KNFTACYIKESIENSTNIKVCIGYGIGNDIKQAMSSAISANKESKINPLNYSFLVNEN
                     YETIGPLLCDKLLTVSNKVTPHINTIAKSSKLSTLTIQKIISVTEILQRDEVTPKELS
                     SCLNVTVRTANRFLSSLLKSGNATILYEKQNSTKGRPERVYKLFISENKY"
     gene            complement(11713..12927)
                     /locus_tag="PC471_002413"
     CDS             complement(11713..12927)
                     /locus_tag="PC471_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HDF2789539.1"
                     /translation="MRNCIIAQSGGPTAVINASAVGIYEKNLETKYFDKVYFGINGIE
                     GILNKNIIDSDSLNKDNVLNLKQTPSSGLGSCRYKLADYKINSYDYDRVINILDEYKI
                     DTLFYIGGNDSMDTVMKFSQYCKNTTCKIKFIGIPKTIDNDLLGTDNCPGFGSAAKLI
                     STLIMETYLDFSIYKNNGIFIVETMGRDTGWLAASSSLARLNDTPLVDLIYLPEIDFY
                     EDKFIEDVRKIFKEKNKAYIVASEGIRDKYGNFICEQKTSAHDKFGHSQLGGVSNYLK
                     NLIVSSSITTRVKTLELGVIQRCAMHLASDTDLKQAAEVGAYGIELSKKGYTGVMAII
                     NRTCNNPYKYEVSHTDISKIANKTKYFPKEWINEECNYITDEALNYLYPLIQGKPDIK
                     YKNGLPYFSIIY"
     gene            13353..14069
                     /locus_tag="PC471_002414"
     CDS             13353..14069
                     /locus_tag="PC471_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentose-5-phosphate 3-epimerase"
                     /protein_id="HDF2789540.1"
                     /translation="MKHIKIAAGLAHVNYGHISAVVKEAMDAGVDYVHSDAADMHDLK
                     NMQLMGGHQIIEAIRPVTDKPIECHIYTRDCDRLFIEKIAAAGCNLLIIPAEHFLGAP
                     LAYIINYCREFGMKVGLTIGCYTPLCFVDEAIYDIDRLQIVVHGVDETDGKDNWGWRK
                     SAVDLVKRARKMIDEKNPKCELAIDGGLRADNMEPLIECNPDVVILSSAIFKDKDGIT
                     AGVKNCRKAIDEAATKFGLE"
     gene            14204..14830
                     /locus_tag="PC471_002415"
     CDS             14204..14830
                     /locus_tag="PC471_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HDF2789541.1"
                     /translation="MKKEMIKDYLLITISAFLMALAVNFFFAEHTLAPGGITGLSVVL
                     STFLSMPVENISLGISMPLLVMGLLFLGKGFGIKTLYITLMGPLFLKVIPQTHITDNL
                     LVAGVIGGLLVGTAIGIAIIRGCSTGGTDLVAMLINKVIKFLKLPVILFMLDGFIIVA
                     SGIISKNFMISIYSLISLLLIIKTIGFVTTKFGKNTEEINNINNACAQ"
     gene            complement(15030..16574)
                     /locus_tag="PC471_002416"
     CDS             complement(15030..16574)
                     /locus_tag="PC471_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HDF2789542.1"
                     /translation="MNKRLLDIAELLLNSSDYITVDTIAEELKVSNKTIRNDLVILDE
                     WLLEFNLSLDKKTGSGVIILGNEDIKLKVIRDINDKSNCIYAYSPEDRKRYILSKLFI
                     KNSKFRIRDICNELHVSRATVHKDLVVIQNFLQNFKVTLVRKTNNGVYLEGKEKDIRK
                     AIFELATVNKSYTELKEILFSKSNECKDTPSTKIFKELFNYDFEKLSQITLHTLQVEK
                     SNLSDEYYINFLIHIAICIKRISIKRYINLSEGFFRELLEHEYFEKSKILCANLSEAF
                     DVEFIDEEVCYILLHIEGLLKSIKKTTTLEPLKNEEITEYNLSEAIAVHWGSILNLNL
                     KDDLILIKSLANHLKSVLHRINYGFTITNPILNDIKRTFPYTYKAAKESNTVIKQLMN
                     YEVNDDEIGYLALYLISAIDRSKSPLNTILICHCSDGVSNLLVQKLSFEFNQINIVKS
                     IPLSSISFTNFDDVDLILTTAPVDFEHNAELININALLSKNDISRLSTIIKKLYSEKN
                     KILSYK"
     gene            complement(16600..17241)
                     /locus_tag="PC471_002417"
     CDS             complement(16600..17241)
                     /locus_tag="PC471_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HDF2789543.1"
                     /translation="MKYLIDSANLDEIQKCLKYGFKGITANPSMYLKEKVNFHEFIET
                     CANLNPEILTAEVIGNNLYELINSCDKILNINKNTIIKINFSEVGLELINILNSKGVK
                     TACTLIFNVNQASLAINAGADYLFPFIGRNDENGNDGIKNLTEICNLISINGYNTKVV
                     AASIKNVFHLTQASLCGCDYAAVTYDLFKKASFNQLTIDGANTFERDWSKVNK"
     gene            17511..17702
                     /locus_tag="PC471_002418"
                     /pseudo
     CDS             17511..17702
                     /locus_tag="PC471_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422425.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
BASE COUNT         6669 a         2128 c         2979 g         6243 t
ORIGIN      
        1 tagtatatct tactagcatt taaatgaaac taaatttgaa gctttaccta ttaatatatc
       61 ttaatagtga ttaaataaaa ctaaacttaa gactttatat gttaatatat cttaagaatt
      121 tatttgttaa aatacagttc attatattta aaagcattac tatgataatt cataatagta
      181 aaatagataa tggttcagac aatattataa atactagtga cggtaaaata gtatcaattt
      241 agtatataaa ccttgtcaga taatcgcata acagagcttg tagttataga ataaaaaaat
      301 atctaggaaa ccgttgacag atttatatgg atagtataac atatagaata tgaaattaaa
      361 caaaaaagaa ttttaattat ggcgacgtag tcataatttt gagtatgtta ctgattagca
      421 ggcagaactc aagtatatat gtgaaaaata gagggatact ctatacatat atgcttgagt
      481 tctttttatt gaagggagga atatatgaaa ataattcaga ttttaaataa taatgtagcc
      541 ttagtaaaaa gaggtaaaaa tgaaattatt gtatttgtta aaggaattgg ctttaaaaaa
      601 agagtgggac aagtaataaa tgaaaatgaa atagagagaa catatatact agattcatac
      661 gatatgctag aacactttag ttatctacta caaaactcag actccaataa tatagttctt
      721 attaatgaaa taattgaata tggtgagaaa actttaaata taaaggcaag tgattatcta
      781 agcttaacct tactagacca tttagagttt ttacttaaaa gatgtaaaaa gaatcaattt
      841 ataaaaagtc ctttggtatg gaatgttaaa agattttatc caaagcattt tgaaattgga
      901 atgcatgcac taaaattaat tggtgatagt aagggaatta atcttcctga tgatgaggca
      961 gtatcaatag cacttcattt tgtaaatatg gaggtaaata aagagtctca tgatagtaca
     1021 attgtagagt tgagaacttt agcagatata gtttctataa taaaatatca ttttaatgta
     1081 gagctagatg aaacttcaac aaattatatg agatttacta ctcatctaca gtactttatc
     1141 caaagaatta tgtcagagga agcttcaata gatgaagata attctataga tttatataaa
     1201 caagtaagtc aactttatcc aaagtcattt ggaagtgttc aaaagataag aaaatatata
     1261 aaatcacaat ttaatgtcga tatatctata agtgaagaaa cttatttaat gttgcatata
     1321 aatagggtga ctcaaagact ggaggcaaaa tcagatgaaa tataaagatt taaatgaaaa
     1381 tataataaaa cttatagggg gaaaagaaaa tataaagtcg gtagcgcatt gtgttactcg
     1441 tttaagattt actttaaatg ataggagtat cgctcaaaca gaagaaatta aaaaattaaa
     1501 aggagtaatt gatgtagttt ctaatgatgt tgcttatcaa attataatag gtactcatgt
     1561 tgtagatgta tataacgagt ttatgtctat gataggttta agttcaagtg aatcaagtat
     1621 aaaaagtgaa agaaaaaagg gaattagagg tatcaaaggt atatttaatt ccatctttgt
     1681 ggtagtctca gagactatga cttcagtaat tgaagtacta cttgcagctg gtatacttgc
     1741 aagtatatta gcacttttaa atatgtctgg catacttcag agtgacagtc ctacatatca
     1801 aatattagat acattaagaa gtgctacatt tcacttctta ccagtattaa ttgcaatagc
     1861 atcagcaaaa agacttaatg taaatccata tatagcagta gtattagcag ttacgttatt
     1921 atcttcatca atagatggtg tagaaggatt aagtttattt ggtataaatt tacagtctac
     1981 aacttatgca aacagtttta ttccaatact tttaggagta tgggcaatgg gaattataat
     2041 tgaaggtttg aaaaaaatat tacctaaagc actacactat tttttagttc cagtattatc
     2101 tttaataata actctatcag ttacattaac tgtatttgga cctattggta tgtggattgg
     2161 tgatggatta aactatgcat gtatgttttt aattgataca ttgggtaatt ggagtgttgt
     2221 tgcactgtat gcagctttac aaccactctt aatagtcttt ggtgcagcaa actttactat
     2281 gcctatatct ttaaattctg tgacaacatt ggggtatgac ccaattttta tgggcgctgc
     2341 aacaatatct gatattgcag tttgtggtgc aatgtttgga tatttcttaa aaactaggaa
     2401 taaagaacaa aaacaattat ttggtacagt aagttttagt gcacttatgg gagttacaga
     2461 accttcagtg tttggtgtgt ttatgaaata tagaagacca tttgtagcag taatgatagg
     2521 tggaggtata ggtggtttaa tagcaggttt agctcaagtt aaaacatatg gaatggtttg
     2581 gggattagct gcattaccaa cgtatcttgt tggaggagag gtatctaact ttacatttat
     2641 gataataagt gtagtagtat catttgtagc agcaacagca gcttcctatt tactaggaat
     2701 accttcagat gaaaaagaag aagttaaggg tgataatgaa aaagaattag aactaataat
     2761 caacgagaac aattcaagaa ctattagtat tggaaatgtt gcaaaaggtc aggtaattgc
     2821 acttggggaa gttaatgaca aagcattttc ctctggagca ttaggtaaag gggttggagt
     2881 tatacctagt gaatcaaaat caactgttat atctcctgta gatggaacta taactatagt
     2941 atttccaact aaacatgcat atggtatagt gactgatgaa ggattagaaa tattaattca
     3001 tattgggata gatactgtaa acttagatgg aaaattcttt gaaagtcttg taacacaaaa
     3061 tcaaagggtt aaaaaaggag atgctttagc agtatttgaa tctgataaaa ttatagagga
     3121 aggctttgat tctactataa taactgtagt tacaaatact agcgattatt tagatgtaat
     3181 ctcatcaaat gatgaatcag aagaattatt aactgtaata gtataaattt taggagggaa
     3241 aattcatgaa tattaaagat acatttttct ggggaggaag tattgcagcc catcaatgtg
     3301 aaggttcatg ggatagtgac aacaaaggac ctgcaattat ggattttgta actaagggct
     3361 cttatgaaat gccaagagta attactgata aaatagaaga aaaattggat tatccatctc
     3421 ataatggaat agatttttat aatagatata aagaagatat agctttattt aaagaaatgg
     3481 gatttagtgc attaagaata tctatagatt ggtcaagaat ttttccaaat ggagatgatg
     3541 aaaatcctaa tgaattaggg attaaatact atgaaggtct gatagataca ttaatagaaa
     3601 ataatataga acctattgta acactatatc attttgaatt acctatgaat ttagtacata
     3661 aatatggttc atggaataac agaaaactta tagatttgta tttaaagtac agtgagactg
     3721 taataagaag atttgatgat aaggtaaagt actgggttac atttaatgag atgaaccata
     3781 ttgaccctca aacagagcac tcggatattt ttacttatat gatagcaggt attaagtata
     3841 gtgaaataga aaataaggtt caaacattgg caaatgtagg gtataatatg actttggcgg
     3901 gtgtaaaggt tgcaaagttg gctagagaaa tcaatcccaa caataaagtt ggatgtgtat
     3961 ttggactaaa tccaatttat ccatacaatt gtaatcctag taatgtgtta aatgcatttt
     4021 tagataatga ccgtgattat tatcaaattg atgcaatgtg caatgggaag tttccacaat
     4081 ataaattaaa agaatatgaa aaaaataata taactattga agtgactgat gaagacaaaa
     4141 aagcatttaa agaaggtaag ttagatttta taggacttaa ttattatttt tcaagtgtat
     4201 ctactccaaa agaaaattta tcaggtgata aatctttatt tggtgggatt caaaatccat
     4261 atttggaaca aagtaagtgg gggtgggcaa tagaccctat aggtttaaga tttaccttga
     4321 actatattta cagaaagtat caattaccta tcttaattac agaaaatggt ttaggggcat
     4381 atgataaaat cgaagaagat ggaactataa atgatgatta tagaattgaa tatttaggta
     4441 agcatctatc tcaaatgaag tctgcaattg aagaagataa tgttgaatgt tttggatatt
     4501 tgatgtgggg accaattgat ttagtcagtg caactacagg tcagatgagt aagagatatg
     4561 gatttatcta tgttgactta gatgatgaag gcaaaggaag ttttaagaga tataagaaaa
     4621 aatcatttga ttggtacaaa gaagtaataa aatctaatgg agaagtttta gatttttcta
     4681 cggatatagt agtagggtag gaataagata aatagaagtt aggaactaat gttaattagt
     4741 gtctagtaaa gcatttaaaa tagagtatag ggtctttaaa gtaaagtgtg tattcttctt
     4801 aaaagtaatg gcaactaaaa ttactataaa aaggagaata cacatttttt atgtttatac
     4861 ttaagtaaat atttagacat attcatagta taatttactc attatataaa aatagtatga
     4921 tttactcact atataacttt ataaattttc tggaaaaatg tcagaatata ttgacaaaga
     4981 aaagatttaa aattataatc tatattaaga atagtttgac aatataaaaa caagtatttt
     5041 tataaatagt gaaattattt ttattgtcaa tttaagaata atttaggact actataaaga
     5101 taaaggtata gatgtcatta tatgataaat tatacttgta tagaaagaat tttaaagtat
     5161 ggcttatcat tgatttaata taataaataa tttttgtttc tagtaatagt tcctaggaat
     5221 aagatatcta aattcgttta gaaatagtct aatttacaaa ggaggggatg cactaataga
     5281 taaaatttct atataaagaa gacttaaagt gaagtatatt ctgataatat tggtttaatg
     5341 ggtatattat tatttcgttt tagatatttt aagaataatt taagacgttt ataaaaatgt
     5401 attctatatc taatatttaa ataaatataa gtcttccaac atgtatacta atatagagag
     5461 tagttgaagt acaattctaa ctcgtaaaat tatgtagaaa gtaaaaatca aaaagaaatc
     5521 tagtaagttg ataactatag ttaatattta gagagttaac tctagttata tttatttaga
     5581 tattttagga ataatttagg acgtatataa tatcatcaaa aaatatcaag ctataccata
     5641 tgtagttaag aataactgca taatatacat tttataatta ttgttttatt gataaaaaat
     5701 tattatattt aggaggaaac aacaatggaa aatttttgga atccacttac ttgctttact
     5761 gtatgtgtaa tgatttatgc ttttagtgag tttttatcta agaaaacaaa aggtgcaatt
     5821 tcaacattgt tatttgtatg tattatattt ttactaggat tttggacaaa tattttacca
     5881 aaagacatta caggagcatc agggatatta gctgcaatga gcaactttgg tatagcttta
     5941 ttaataacta acttaggaac acttataaat cttgaagact tatgtcatga atggaaaacg
     6001 gttgtaattg cattagtagg tattgtagga ataggagttg gctgtttaac agtgggtagt
     6061 atgttatttg gcagagagta tgctttaata gcagcacctc caatagcagg ttctactgtt
     6121 gctgggatta tcgtaacaac tgttgcagaa gctgcaaata gacctgaact tgctgcattt
     6181 gtaattctag tattatcatt tcaaaagttc tttggtattc cgataactac atattgtata
     6241 aataaagaat taaaatttaa aagagataac ggggattttt tgaaagatga gtcaaaaagt
     6301 aattttaaac ttccaagtat gagaattttt aaagaagatt taggtaaaaa atcatctact
     6361 ctagtgtatc ttgcaaagct ttcatttgtt gctatgattg gtaattttgt aggattggct
     6421 actttaatac caggatttac ccctgcaaac tattatttaa atcctaatat agcatgtttg
     6481 ttagtagggt tgatttttac acgaataggt tttttagaaa aaggaatttt agataaatct
     6541 caatctaatg gaattataat gtttggttgt atgttgatgt tacctggagg acttgctaag
     6601 gtaactccaa cagcattgtt tgggatgatt gttccaacta taggaatact tgttgtaggt
     6661 tctgtattta taatattatt tactgcaatt gtaggaaaac tattgggata ttctccaaga
     6721 atgtcagctg caattggtgt aacttgtatg ctagcttatc ctgcaacaca gataatttcc
     6781 aacgaaggta ttgatgctat ggaaggaact gaagaagaaa aacaaagggc aacagattat
     6841 ttattaccaa agatgattat aggaggattt gttactgtaa ccattgcatc agtggcattt
     6901 gctagtataa taggtccaat tatatttggt tgatagtaaa tatacttata ggaggaatag
     6961 gaaatgatag gaagtatatc tataaatgag atagataaaa aaagattcaa acttgatgat
     7021 ttaagtaaga aaatatggga aaatccagaa aaagcattta aagaatttaa agcatgtgaa
     7081 aatacagcaa actttctaag aagtgaagga tttgatgtgg aagtaggtgt aggaggttta
     7141 gccacggcta ttagagctag ttttggttca ggtaaaccag ttataggttt tatgggagaa
     7201 tttgatgcat tacctggttt aaatcaaaaa gtttcaacaa agcaagaagc atttgaattg
     7261 ggtgcatatg gtcatggatg tgggcataat ttattatgta cagcacatgt aggggcagta
     7321 gtagggctta aaagagagat gatagaaaat aacttaagtg ggactatagt attttatgca
     7381 tgtccaggag aagaacaatt aacaggtaaa ggatttatgg ctagaggtgg agcgtttgaa
     7441 gggttagact tagctataaa ttttcatcca aataaaatta gtgaagctac aattggtact
     7501 tcaacagctg ttaactctgt aaagtttcac tttaagggaa agactgcaca tgcagggtca
     7561 gaccctcaaa atggaagaag tgcactagat gctgtggaac ttactaatgt gggagctaat
     7621 tatttgagag aacatgttac ttctgatgta aggattcatt atacaattac agatggagga
     7681 gtagcaccca acatagttcc tgataaagca tcagtatggt attatacaag agctttaagt
     7741 agagaagctg tagaaagtac atatgaaaga cttgtaaagg tagcaaaagg agctgcaatg
     7801 atgactgaaa ctgaagttga agtagagttt ttaggagggt gttataatac tttaaacaat
     7861 catgttttag caaatcttgt atcagaatgt atgaatgaga ttaaacaaga gccttggact
     7921 cgagaggaat tggattttgc agctgaatta gatagacaaa gcccacaaca atataaggct
     7981 atgataagta aatataattt gccagaagga actcatctat attatggtgg aggaaatgtt
     8041 acaaacttta atagttatgg ctctactgat attggagatg taatgcatat agttccgaca
     8101 gcatactttt ttacagggtg tacgaatcta ggagcaccag gtcatagctg gcagtttaca
     8161 tcttgtgcag gaagttctat aggagaaaaa ggtatgatat atgcatcaaa gattatggct
     8221 atgtttggag ctaaaatatt aaataatcca gagatagcta aaaaggcaaa agaagaattt
     8281 gataaatcta tgaatggtaa aacatataaa tgtcctattc cagatgatat tccaactcct
     8341 taaaaagaca tgaaatttaa tatataaaat tataattttt atatcagcat catttcatta
     8401 ctaaaagaaa gtgagatttt gctgactatc tgattaggag gaaggaatat gaaaaatttt
     8461 tttaaagaag cgcagtcaat agaaggggaa attattagat ggagaaggta tttacatgaa
     8521 aatcccgaaa ttggttgcaa tctaccgaaa acagtaacct ttgttaccag tactttagaa
     8581 aaaatgggat attctccgaa attgacgtct aataatggaa tagtagctgt tttagacagt
     8641 gaaaatggtg gaaagacctt attattaagg gcagatatgg atgcacttcc tattagggag
     8701 gagacggatt taccttttaa aagtaagaat aaagatgcat ctcatagttg tggacacgat
     8761 aatcacacag caatgctctt aggagcagct aaaatactta tggataataa gcacttactt
     8821 aaaggtagag taaagtttgt atttgaacca gatgaagaga ctaccagagg tgcaaaatca
     8881 atgattgatg atggtatatt agaaaatcct caggtcgatg cagctatggc atttcacagt
     8941 atggtgggta aatttcttaa cacaggtcaa gtagcttatt cacgtggtcc agctatggca
     9001 tctgcggata tatttgaaat aacagtagaa ggaaaagcaa ctcatggagc aagccctgaa
     9061 tttggagtgg acccaataaa cataatgtcg catatacaaa tagcattaaa cactatactt
     9121 tcaagagaaa aacctcagaa agagcctgta gttttaacta ttggtatgtt taatgcagga
     9181 aacgtaccaa atgctatacc aaataaagca tctatgtcag gaactttaag aacatttgac
     9241 caatttgtta gagaaaaatt aaaaaataga attattgaaa tatctacagg aatagctaaa
     9301 tcatttggag gaaatgctaa agtggagttt aaaatgggga ctcctgcagt tataaatgat
     9361 ttggatgtag gagatgagat tgtatcttat gttaaagaag ttataggaaa tgaaaatgta
     9421 atagactttc catctgcaat gggttcagaa gatttttcag aggttttact aagggttcca
     9481 ggagtatttt tatggattgg aatgggaagt attagtcagg gatataattt tggtatgcat
     9541 aatcctaaag caatttttaa tgaagaggga ttagtttatg gttcagcttt atttgctcat
     9601 tgtgcagtaa aatggttaga aaataatttt agtagataat aacataaaat attattaaat
     9661 atcataaaag gtatatctta aaaagatgca aagatatacc ttttagaaag tcatatataa
     9721 ttttacttaa tattagatga taaaatattt ttatgcattt tggattttaa tatttatttt
     9781 ctgatataaa taatttgtat accctttcag gtctgccttt tgttgagttt tgtttttcat
     9841 ataatatagt agcgtttcca gactttaaga gactacttag aaatctatta gcagttctaa
     9901 ctgttacatt taaacatgaa gataattcct taggagtaac ttcatctctt tgtagaattt
     9961 cagtaacaga tataatcttt tgaatagtaa gagtagacaa tttagaactt ttggcaattg
    10021 tatttatatg tggagttact ttatttgaaa cagtaagtaa cttatcacac agtaaaggtc
    10081 ctattgtctc ataattttcg ttaactaaga aactgtaatt taaaggattt attttagatt
    10141 ccttattagc acttatggca ctagacatag cttgctttat atcattacca attccatatc
    10201 caatacatac tttaatattt gtactatttt caatagattc ttttatataa catgcagtaa
    10261 aatttttagt taatgattta atcgtttcac atttagtgaa aattttaaaa gaattactat
    10321 ttttttcaat tataaaatcg gttaatttat cttttttaat actatttaat attttataaa
    10381 cttggtctag agtagagtca tcataatttg tatctttaac tgttatatat attacacttg
    10441 gttgatttcc cttcattcta tgtaaatgaa tatcttttat taagttatta aaagtatttt
    10501 ttaataaatc tatatcaggg tatatgaaat gaaagtttac tcctgcattt tgtaacatag
    10561 gaacaatact actaaaacga gtagtagaaa aatcgatttt cccttcattc catagattaa
    10621 tatgtttttt tgcaatcttt ttttcaagtt ttgctatgtc agataaactc attttagatg
    10681 cattttcatc aatactatcc attaagtctc caaatgtgcc atttgataaa taatatgata
    10741 tatcttttgg attatctgcc cagtctgtta agtcaaagta tgctctggaa aaatctaatg
    10801 tattatattt aaagataagc tttagaaaca tatcatagta actttgtaag tcagtaccaa
    10861 aatcaactag aggtttttta atagttccag ccttttttgt aatagctttt ttggggatgg
    10921 ggccactaac tgcaaatcca tcaaacctat cttctatttc taaatataaa tctccaacat
    10981 gagaaaaatc tctatagata tatatttcaa tttctgcatt aatatttaac ttttttatag
    11041 aattatctac aaactctttt aaaaattcgg ttgttatgac tgctattttc atcatagatt
    11101 acttcctttt caattattat aattatttta tattaaatat tacattaatc tattgttcta
    11161 gttaaaatcg tatttcaatt aaattcaatt aaaataaata actctttata aaaagcaatt
    11221 atttaggtag aggaatattt gctaacttta atagtctttg ttaactttaa ttttatttaa
    11281 gttattatag tcttactaat tttataattg tagatttaac tactttatgt tcttttattg
    11341 tttatgtagt tgatttagaa gttattttaa ccatttattt taattgaagt tgtttgactt
    11401 ttaaatcctt tgtgtaatct ttgtttttct agccatgata aaagtatttc tatttgtatc
    11461 actatatata ataatatcat gacaaaatat ttattgtcta actaattata atctatccaa
    11521 aatgaaaatg ggtgtgagat attattacaa caaattgtat tagtattaaa aagattaaaa
    11581 aattaaaaaa ctaaacagtt aaataattaa acgattaagt gactgtcata taacaattta
    11641 taattttatt taacagccac ttaggtattt aagagcgata gaattgagtg tatatcaaat
    11701 tataataatt tcctagtaaa taattgaaaa ataaggtagt ccatttttat atttgatatc
    11761 tggtttacct tgaataagtg gatatagata atttagggct tcatcagtta tataattaca
    11821 ttcttcattt atccactctt taggaaaata ttttgtcttg ttagctattt tagaaatatc
    11881 agtatgacta acttcatact tataaggatt attgcatgtt ctattaatga tagccataac
    11941 tcctgtatat ccttttttag atagttctat cccatatgca ccaacttcag ctgcttgttt
    12001 taaatcagta tctgaagcta aatgcatagc acatctttgt attacaccaa gttctaaggt
    12061 ttttactctt gttgtaattg atgatgatac tataagattc tttagatagt tgcttactcc
    12121 acctaattgg gaatgaccaa atttatcatg agcagaagtt ttttgttcgc atataaagtt
    12181 gccgtattta tcccttatac cttctgatgc aactatataa gccttatttt tttctttaaa
    12241 tatttttcta acatcttcta taaacttatc ttcatagaaa tcaatttctg gaaggtatat
    12301 taaatcaaca agtggagtat cattaagtct agctagtgaa gaagaagctg ctaaccatcc
    12361 agtatctcta cccatagttt ctactataaa tataccatta tttttgtata tagaaaaatc
    12421 taagtatgtt tccattatta gtgtagaaat aagctttgca gcagaaccaa atccaggaca
    12481 gttgtcagtt cctaataaat cattatctat tgttttagga atacctataa attttatttt
    12541 acaagtagtg tttttgcagt attgagaaaa tttcatcact gtgtccatag aatcatttcc
    12601 gcctatataa aataatgtat ctattttata ttcatctagt atatttataa ctctatcata
    12661 atcataagaa tttattttat aatcagccag tttgtatcta caagaaccaa gaccagacga
    12721 aggtgtttgc tttaaattta atacattatc tttgtttaag gagtctgaat ctattatatt
    12781 tttgtttaat ataccttcaa tcccattgat accaaaatat actttatcaa agtatttagt
    12841 ttctaaattt ttttcataaa taccaactgc agaagcattg ataacagctg tagggcctcc
    12901 agattgggca ataatacagt ttctcatttt acatctccct ttcattaata ttgttatgaa
    12961 tggtactttt tatcgagtaa atttatattt atagtctgat tatatattaa atgaactagc
    13021 tatatcaatt gagttagtat agacaataaa tgccataaga atgtggtaaa aaactaactt
    13081 tttatataca attaatgtct ataatttacc acaatataaa ggtactttta aggagtagtc
    13141 aaagtgttgt gaaaaaaaat atatcagtgt aatattaatt tacaaagcaa aataagttca
    13201 cagttaacaa aagcagaata agttcataaa agaaatatat acagcaataa aagtaatgct
    13261 aaataaaagt aatactggat taaaaaatat taaaaaaata ttaaaaacta atatttaata
    13321 ctaattaaag taaaatatgg aggtatacaa atatgaaaca cataaaaata gcagcaggat
    13381 tagcacatgt aaattacggt catatatcag ccgtagttaa agaagcaatg gatgcaggtg
    13441 tagactacgt acattcagat gcagcagata tgcatgactt aaaaaatatg caacttatgg
    13501 gtggtcatca aataatagaa gcaataagac cggtgacaga taaaccaata gaatgccata
    13561 tatacacaag agattgtgat agattattta tagaaaaaat agcagcagca ggatgtaact
    13621 tgttgataat accagcagaa cattttttag gtgctcctct tgcatacata ataaattact
    13681 gtagagaatt tggtatgaaa gtaggattaa caattggatg ttacacacca ctatgttttg
    13741 ttgatgaagc tatatatgat atagatagat tgcaaatagt cgttcatggt gttgatgaaa
    13801 ctgatggaaa agataactgg ggatggagaa agagtgctgt tgatttagta aaaagagcta
    13861 gaaaaatgat agacgaaaag aatcctaagt gtgaattagc aatagatggt ggactaagag
    13921 cagacaatat ggagccatta atagaatgta atccagacgt tgtaatatta tcttcagcaa
    13981 tattcaagga taaagatggt ataacagcag gtgttaagaa ttgtagaaaa gctatagatg
    14041 aagcagctac aaagtttggt ttagaataag ataataaact atgataaaag agatgctttc
    14101 ttaatgtttt aaggaagcat taattttatg aaaaactaca ggaagctaga ctaatttaat
    14161 tgatttttta tactgaaaca atataatcat aggaggttat taaatgaaaa aagaaatgat
    14221 aaaagattat ttactgataa caatatcagc atttttaatg gcattagcag ttaatttttt
    14281 ctttgcagaa catacattag ctccaggagg gataacagga ttatcagtcg tacttagcac
    14341 attcttatca atgcctgttg agaatatatc attaggaata tctatgccac ttttagtaat
    14401 gggattatta tttttaggga aaggttttgg aataaaaaca ttatatataa ctcttatggg
    14461 cccattattc ttaaaagtaa taccacaaac tcacataact gacaatctat tagtagcagg
    14521 agtgataggt gggttattgg taggaactgc tatagggata gcaataataa ggggctgtag
    14581 tactggtgga actgatttag ttgcaatgct tattaataag gtaattaagt ttctgaagtt
    14641 gccagttata ttatttatgc tagatggatt tataatagta gcgtctggaa taataagtaa
    14701 gaattttatg atatctatat attcattgat atctttatta ctcataataa aaacaatagg
    14761 atttgtaact acaaagtttg gtaagaatac tgaagaaatt aataatataa ataatgcttg
    14821 tgctcaataa aaataataaa atttacttta aatataaaag tttaaaaaat ataaaagctt
    14881 aaataaaaag attaaatata aaaaatgaag gtgtctcaaa atgaattttt tagttcatga
    14941 ggcacttttt ctatgaaatc aaatatattt ttatcatttg gacaatgaaa aataggttta
    15001 tctctatgtt gagacaagcc tatttttgtt tatttataag ataatatttt atttttttca
    15061 gaatatagtt tttttataat tgtactaagt ctagaaatat cattcttact taaaagagca
    15121 tttatattta tcagttcagc attatgttca aagtctacag gagcagtggt tagtattaag
    15181 tccacatcat caaagttcgt aaatgaaata gaagatagag gtatagattt aactatatta
    15241 atctgattaa attcaaaact taacttttgg actaataaat tagatacacc atcactacag
    15301 tgacatatta aaatagtatt taggggagat ttgcttctat ctattgctga tattaaatat
    15361 aaagcaagat atcctatttc gtcatcattg acttcataat tcatcaactg tttgataaca
    15421 gtattagact ctttagcagc cttataagtg taaggaaatg ttctctttat atcatttaat
    15481 atgggatttg taatagtaaa cccatagttt attctgtgta gaactgattt aaggtgatta
    15541 gcaagagact ttattaatat taaatcatcc tttaagttta aatttagtat acttccccaa
    15601 tgaacagcta ttgcttcaga tagattatac tctgtaattt cttcattttt aagaggttca
    15661 agagtagtag ttttctttat tgatttaagt aatccttcta tatgaagaag gatataacag
    15721 acctcttcat caataaattc tacgtcaaaa gcttcactta ggttagcaca taaaattttt
    15781 gatttttcaa agtattcatg ttctaataat tctctaaaaa aaccttcaga taagttaata
    15841 tatcttttaa ttgaaattct tttaatacat atagctatat gtattaagaa gtttatataa
    15901 tactcatcac taaggttact tttttctact tgtaaggtgt gtagagtgat ttgagataat
    15961 ttctcaaaat catagttaaa tagctcctta aatatttttg ttgatggggt atctttgcat
    16021 tcattagatt tcgagaataa tatttcttta agttcagtat aacttttatt aaccgtagca
    16081 agttcaaaaa ttgcttttcg aatatctttc tcttttccct ctaaatatac tccattattg
    16141 gtttttctaa ctagagtcac tttaaagttt tgcaaaaaat tttgaataac taccaagtct
    16201 ttatgcacag tagctctact aacatgtagt tcattgcaaa tatcacgaat cctaaactta
    16261 gaatttttaa taaataattt acttagaata tacctttttc tatcttcagg agagtatgca
    16321 tatatacaat ttgatttatc attgatatct ctaataactt taagttttat atcttcatta
    16381 cctagaataa taacccctga accagttttt ttatctaggg ataaattaaa ctctaaaagc
    16441 cattcatcta aaataacaag gtcattacga atagttttat tactaacttt taattcttcg
    16501 gcaatagtat ctacagtaat atagtctgag ctgttgagta ataattcagc aatatctaat
    16561 aatcttttgt tcaatgcaaa ccctcctaaa tcttagttac tatttattaa ccttggacca
    16621 atctctttca aaagtattgg caccatctat tgtaagctgg ttaaaagaag cttttttaaa
    16681 taaatcatat gttacagctg cataatcaca tccacaaaga gatgcttgag ttaagtggaa
    16741 tacattttta atagaagctg caacaacttt agtattatat ccatttattg atataaggtt
    16801 acatatttct gtaaggtttt ttataccatc attcccattt tcatcatttc tacctataaa
    16861 agggaatagg taatcagcac ctgcatttat agctaatgat gcttgattta cattgaaaat
    16921 caaggtacat gcagttttta cacctttact atttaatatg tttataagtt ctaagccaac
    16981 ttcagagaaa tttattttta ttatagtgtt tttatttata tttaatatct tatcacatga
    17041 attaattaat tcgtataaat tatttccaat gacttcagca gttaatattt ctggatttaa
    17101 attagcacat gtctcaataa attcatggaa gtttactttc tctttaagat acatagatgg
    17161 attagcggtt attcccttaa atccatactt tagacacttt tgaatttcat caagattagc
    17221 actgtctatc aaatatttca taatttaatt ctcctttaag ctatttgtca taaaaaagtg
    17281 tatatactct atttttatta ttaagtcaat tatacaataa gattgattat aaaattataa
    17341 catgggtact taataaaatt tacataatga tatggtaata ataaaacatt gattgtctaa
    17401 taattaccat aagctttagt aaataagagt taacagtgaa ggtattgcct taaaaatcct
    17461 atgatatgta taatttgaat atgaaggcaa taatttaagg gaggaaaaat atgattaact
    17521 tttatcaacc atcaagaata aattttggac aaggaacttt aagtgaatta cctagaataa
    17581 taggaaaata tggaaagaaa tgtttgttag ttactactcc aaatgtagag ccattagaca
    17641 agttatataa aagagttaca ttgaatttaa tagaaaaagg tatagaagtt gtacactttg
    17701 attaaaaaat agctacgcac actcgtgact ttagtcatga gttaatagct aaaatagtca
    17761 gcatataggg aaacctatat gtagagatag gatagaacct atccaatact acttgaattg
    17821 ctggaaaccc ctaaagctaa ccaaactaaa acataaggat gaaataaacc taagtgtgaa
    17881 agttacgaaa gtagaaaaaa ttggttagat ggtataaggt taaatcctaa gtactttaaa
    17941 atgggcaatc agcaggtaag ctccgaatag gagaaacttc aacgactatt cctcttgagg
    18001 gaagtacact acaagttat
//
LOCUS       DAMJYR010000074        17995 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.074, whole genome shotgun sequence.
ACCESSION   DAMJYR010000074 DAMJYR010000000
VERSION     DAMJYR010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 17995)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17995)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17995
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.074"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            <1..498
                     /locus_tag="PC471_002419"
     CDS             <1..498
                     /locus_tag="PC471_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDF2789544.1"
                     /translation="KSLNKLAKLQRELSRKTIGSLNRNKARLKVARLQEHIANQRNDF
                     LQKLSTKLIKENDIICIEDLQVKNMIRNRKLSRSISDVSWSEFIRQLEYKANWHGRQI
                     VKVGKFFASSQICNKCGYKNEEVKDLNIREWIYPSCNETHDRDINASINILKEGLRLI
                     TIQNK"
     gene            930..1184
                     /locus_tag="PC471_002420"
     CDS             930..1184
                     /locus_tag="PC471_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2789545.1"
                     /translation="MENKDIKLTICIFAFVIGIIVLFCCIGLGQFNMDCIIKQNGGSI
                     ATEQYNIYLEQSISQYRNFGSILVLLGGIGILFDKSVRKY"
     gene            1220..1543
                     /locus_tag="PC471_002421"
     CDS             1220..1543
                     /locus_tag="PC471_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2789546.1"
                     /translation="MARDVFSVKMTPLEAQKMVEKNLEADLVSVDIYNLENNKSIIVT
                     IFEKYYARSNSDAGLVVICENTTGKTLVKITSTGSAIGLFQIDWGTGNNLIKRVKKIL
                     IDYII"
     gene            1817..1969
                     /locus_tag="PC471_002422"
     CDS             1817..1969
                     /locus_tag="PC471_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2789547.1"
                     /translation="MDNFLFNVLASVTASYIVYLISKLIKKVKSHSTAKSDLKIDINF
                     KYHRKK"
     gene            2163..3086
                     /locus_tag="PC471_002423"
     CDS             2163..3086
                     /locus_tag="PC471_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HDF2789548.1"
                     /translation="MVGAFVLPNYAIILSEMSNLWGNFIMWILVPIITIVLLIIAVSS
                     MQYILVMIAFLLIIYSFIEKKIVMGLVSVLFFTYSIYLCATWEDKSLIADNKVETVRA
                     QRETVEREKEMERRRIQEEVDKERYIEKHGMEISENDLKVKLEALVPQEYKGKKYELK
                     VGKFKRYSMYFDLTVQNEKFSNSEECKKFVKEIANDLKKIKISKAYFKFHSKDDGGIY
                     NSVYIDYFRNIQNNVDNVENLEFNEFELKTEEEEKREQEKIEQEKNSYNNYIQNRVVD
                     PLDRIKKLKELLDSGAITQEEYNKKKKELLE"
     repeat_region   3353..4300
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=3353..3381
                     /rpt_unit_seq="gttttagattaactatatggaatgtaaat"
     gene            4491..4643
                     /locus_tag="PC471_002424"
     CDS             4491..4643
                     /locus_tag="PC471_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2789549.1"
                     /translation="MKNCRKDKRGFSVLLDKEKLDKFDEVLEEKNLTKKEWLEEKIDE
                     ELEQKE"
     gene            4763..5576
                     /locus_tag="PC471_002425"
                     /pseudo
     CDS             4763..5576
                     /locus_tag="PC471_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894376.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="toxin Bro"
     gene            5628..5789
                     /locus_tag="PC471_002426"
     CDS             5628..5789
                     /locus_tag="PC471_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2789550.1"
                     /translation="MCENLLDNMYIEKRKEEYRIKLLKIRETDIDIYNKLESIVYKLS
                     EKKLEKNNK"
     gene            complement(6117..6581)
                     /locus_tag="PC471_002427"
     CDS             complement(6117..6581)
                     /locus_tag="PC471_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2789551.1"
                     /translation="MDFGEVKSELSDKDLKDNEEALNKYKKYYLKVYKYYSEMDSIKS
                     IQDIEIEQSRLKGQLQKNSNPIFALYISIMAVVFSTTINLTFDLSDITNYFIRMIMTL
                     LFIVLLIFLVNKGILPIAIPYRNNIQFYTTALDVLEDMKNKILRERKYKKRR"
     gene            complement(6717..7034)
                     /locus_tag="PC471_002428"
     CDS             complement(6717..7034)
                     /locus_tag="PC471_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2789552.1"
                     /translation="MDFGNINLILIGIIVIIGTTIIYLIKPKTAFCSKKYFNKLESIY
                     GNIDKKKTVKLEVLYRYVTGLEYIAIGLFTRRLDITIIAIILVAIITTALYYLIRKKY
                     ITI"
     gene            complement(7317..7613)
                     /locus_tag="PC471_002429"
     CDS             complement(7317..7613)
                     /locus_tag="PC471_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2789553.1"
                     /translation="MYFKTYILKREVNNKQLEITRSFDLDNLICTATEILKDNEIDLI
                     ITDEVNNVVWRNEKSTPPARECLVIAIEVYWWYTSIFLLYHINYVIKKNYLNRF"
     gene            complement(7632..8045)
                     /locus_tag="PC471_002430"
     CDS             complement(7632..8045)
                     /locus_tag="PC471_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2789554.1"
                     /translation="MKVKSKRSFIVGIIVCVLCCASLVIYCILKDKRFLISSFLLIVI
                     AIFNFCNAFSRKGIVEELHDSTDERDLYLTMKTSHILVKIMNYTLFTFTFLFIIAYSA
                     WKNQSLLVIAITLCVIEIFLFVAYLLINIFLEKKE"
     gene            complement(8029..8262)
                     /locus_tag="PC471_002431"
     CDS             complement(8029..8262)
                     /locus_tag="PC471_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2789555.1"
                     /translation="MKTKVREFRTNMGLTQQQLADLVHVSNRTIISIEKEQYSPSLML
                     AYRMAQVFGTTIEELCCLKENKEMEDKKHESKE"
     gene            8481..8855
                     /locus_tag="PC471_002432"
     CDS             8481..8855
                     /locus_tag="PC471_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HDF2789556.1"
                     /translation="MKISKLPEAELKVMRYICEANKVLTSRELVAAMEEKYEWNESTT
                     FTVLKRLERREFLSTEKIGKLTHYNILVKEKKYLRFETKEFLKNIHKNSISSLMSALH
                     GEDDEVDEDKLSELEECFKNLK"
     gene            9467..10159
                     /locus_tag="PC471_002433"
     CDS             9467..10159
                     /locus_tag="PC471_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2789557.1"
                     /translation="MYNILVVDDDKEIVDSIEIYLKSEGFKIYKAYDGMKALEIITEN
                     DIHLILMDIMMPKLDGIKATVKIREERNIPIILISAKSEDTDKIIGLNIGADDYITKP
                     FNLLELIARVKSNLRRYVTLGNYEDTCKEILKSGALELNTLTKEVKIDGENIKMTPIE
                     YKIIELLLENKGRVFSIDEIYEKVWNEESFNSENTVAVHIRRIREKIEINPKEPRFLK
                     VVWGVGYKIEKF"
     gene            10355..12415
                     /locus_tag="PC471_002434"
     CDS             10355..12415
                     /locus_tag="PC471_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HDF2789558.1"
                     /translation="MEKYRVLKLVSLIIISISFPLTILLVTIGLAEPKYNIQKTSTKS
                     GIFDISDDFYDSNIFDEGTMASLLPKFKLSVGGKLTSKEKGKFQQEGKSIDLSSSDSE
                     YDKIMINEQASEEYLSYLKNIKVYVINTYTGVFYTNTSYASLYDFKQKTKSYCNVEII
                     NKIGKSSYIKTINGEKFELNNLSLELNEVDEGFEAYVSFPEEPTIEDGIVYTNFQIFK
                     QATEKVRLFFVASLSLAIICLLSIILYIKIKGEVLKRNNVFLLIYSKIPMEFNVILVS
                     ISIAILTIYALAVSYHIDVIALVSLSLLIVSNNIFFINVQLSYLENKMCIFKTSIIVR
                     VILWLIKTVREVSNAIKKVSLARKVIVIVIISLALIMAISTRSIGMGLLFSICIFLGF
                     TIYITKRLSYLSYVIEGTERIKKGELDYKIKITGNDNFTSLAENINNIGEGLDRAIEE
                     QVKSERMKSDLITNVSHDLKTPLTSIINYVDLIKKEESIQPEYINDYINVLESKSKRL
                     KLLIEDLFEASRVSSGNIELQISKIDLVQLLRQSIGELEEKLSKNNLYLKLNVPNDKV
                     YIWADGRRMYRVFENLLSNIAKYSLEETRVYIDVYDYGENVKVTMKNISSYELNFDPS
                     EIMERFKRADESRNTEGSGLGLAIARDLVALQGGKFYIEIDGDLFKANLEFQKYEEEI
                     TNME"
     gene            complement(12620..12943)
                     /locus_tag="PC471_002435"
     CDS             complement(12620..12943)
                     /locus_tag="PC471_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HDF2789559.1"
                     /translation="MNNNYIFFIIIGMYIVTYLPRVLPMLIFSKKEMPDFLKKIMKFI
                     PVAILTSLTAKDVFFNGDSLYLSIANPKIISFMIVLLVAYKFKSIGISIITGVISIYL
                     FGIIL"
     gene            complement(12936..13637)
                     /locus_tag="PC471_002436"
     CDS             complement(12936..13637)
                     /locus_tag="PC471_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HDF2789560.1"
                     /translation="MERTQQLKESFIASIPIFLGYIPIGIVGGILLQKSGLSPFQIAL
                     MVTLVFGGSSQFIAASMISTGASVTSIVLTTFIVNLRHFLMSSNLNMYIKNKSPKFIL
                     PFCHTITDETFAVNYEKFTAGNWSDRNAIYLNFFCLISSVLANFIGAFLGESISIDSS
                     ISGFILTSMFIALIVSQIKNKIYIVVFISSIIISVILYALFKSNLVIIVASILASLIG
                     FFIEESYCKKEESYE"
     gene            complement(13915..15300)
                     /locus_tag="PC471_002437"
     CDS             complement(13915..15300)
                     /locus_tag="PC471_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HDF2789561.1"
                     /translation="MPINSFENYPMNWKPKRPSKGQILYKALAEQLEQDINNGFLLPG
                     TKLPPQRELADFLDVNVSTISRAFKICEKKGLISGVTGSGTFVSYDTRSNLFLMSSNN
                     KITFIEMGTMNPDFTLEEMNTLFKHIIKEIDFKTIFQYGQRDGAKWQKEAIAKLIYKA
                     GLETTSDSLLPASGGQNAIVAILAGLFQHGDRIGVDPLTYPGIKTAAKMLGIQLIPIK
                     QEDNEISEEGLLYACKNENIKGLYIIPDYQNPTTHIMSQNGRKMIANIASKYNLIVIE
                     DAIHSLLNETHLNPVASYLPNQTIYITSLSKIIAPSLRLAYISTPKQYREALSSALYN
                     INLSQSYFLTEIAYRMITSGEADKLINARRKSARRRNKIINQYLSGYNLLGNEECIFR
                     WLILPDGIMAEKFEIQALKEGVQVYASERFAVGKEKPISAIRIAVCATESTEELKAGL
                     SVLKRLLEEKK"
     gene            complement(15408..16646)
                     /locus_tag="PC471_002438"
     CDS             complement(15408..16646)
                     /locus_tag="PC471_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HDF2789562.1"
                     /translation="MELFIEVIKFNIYISPLILFFILFTKKISKYTFKFNYFICLLIV
                     VRMMFISKINLNILNINNTTINIYKHNIDNYIPYDQGITLDIFMYIWILGIIAISFYY
                     LRTNFKFYNYIENFKKEILEIDITSILDEQAKELSINKKLQIYKVKGIYSPAIIGLTR
                     CKIIIPEKKYNNKELRFIFKHELIHYKRKDNLFKLLISIICIIYWFNPILYLLKKYFH
                     ELCELSCDEIVIDKCTTSEIKEYAYIILDTIKYQKSLKYSSFVSQFLNKKNIILKKRL
                     DSMFNQDKKKINLGLKFLLTFIIIGSIFSINSNFKQNEDVEYTIGKINYNKNGETYGT
                     HIIDSNGNSIEPELIQTIGRGKKVGYVKKRDLYDCDNQPKTPEEAVLYTRKRNFNIFK
                     KTIPLYDKEGIKIIGRFEIN"
     gene            complement(16659..17027)
                     /locus_tag="PC471_002439"
     CDS             complement(16659..17027)
                     /locus_tag="PC471_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HDF2789563.1"
                     /translation="MKKIPKSELLVMNFIWDKDIDKIPAREVANYMEISHGWTKGTTG
                     KVLSRLSDKGFLEFEKDGRNTLYTIKVKHEEYLKFETKDFLELIHKNSIHSFISSLGC
                     GNEISDDDIKELEQWLKKRK"
     gene            complement(17555..17977)
                     /locus_tag="PC471_002440"
     CDS             complement(17555..17977)
                     /locus_tag="PC471_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HDF2789564.1"
                     /translation="MNTFKTEVLLKYSAKDIFNIFTKSARLNFPNFNEKKAVGTFVQQ
                     KNNKRFKVEITNFEKNRVYEIKTSNKRESTITRYELVHIDLENTKLIFIESESKRNIF
                     GKINSALVRVLFGKRQPEEFNKFIKKLEMELENNSVNL"
BASE COUNT         7015 a         1994 c         2634 g         6352 t
ORIGIN      
        1 aagtcattaa acaaacttgc taaattacaa agagaattat caagaaaaac aatcggtagt
       61 ttaaatagaa ataaagcaag attaaaagtt gcaagacttc aagaacatat agctaatcaa
      121 agaaatgatt ttctacaaaa actatctact aaattaataa aagagaacga tattatttgc
      181 atagaagatt tacaagtaaa gaatatgatt aggaatcgta aactatctcg ctcaatttct
      241 gatgtgtcat ggtcagagtt tattcgtcaa ttagaataca aggctaattg gcatggtaga
      301 caaattgtaa aggtgggtaa attctttgca agttcacaaa tatgcaataa atgtgggtat
      361 aaaaacgagg aagtgaagga cttaaatata agagaatgga tttatcctag ttgtaatgaa
      421 actcacgata gagatataaa tgcaagtata aatatattaa aagaaggatt aagactaata
      481 acaattcaaa ataaataacg tatgtaagaa ccgtaggaac tacggggata gcctgtggag
      541 aattagtaag acatatgtta gtatgcaaaa ttctgtgaag caggaaacct gtgactttag
      601 tcatgggagg ttcaggctag tattactcat tattgtattc aaaagctaaa gtggaaacct
      661 agtgaatata tgaatttaga agttaatgag agagcattag cagccgactc aatacttatt
      721 aaaattgaag atgaagaaga aacaatgaaa gaagctgaaa gagagaaaaa gagaggtaga
      781 agaaggtaga atttaaagtt aaaatagtta cattgctaat atatgaaaat gttacaaatt
      841 tggaaataga tggatatata tgttgtatag tataatgtag ataatatatc accaagtaaa
      901 aaacaacaaa taaatctaaa ttatgagggg tggaaaataa agatattaaa cttacaatat
      961 gtatatttgc ttttgtaatt ggaataatag tgttattttg ctgtataggt ctaggtcaat
     1021 ttaatatgga ttgtattata aaacaaaatg gtgggtcaat agctaccgaa caatacaaca
     1081 tatatttaga acaatctatt tcacaatata gaaattttgg ttctatactg gttttactag
     1141 gaggaatagg aatactgttt gataaaagtg tcagaaaata ctaaaaaagt atatttttat
     1201 ttaggatgtg atgaatgata tggcaagaga tgtatttagt gtaaagatga caccactaga
     1261 agcacagaaa atggtagaaa aaaatttgga agcagattta gtttctgttg atatttataa
     1321 cctagaaaat aataagtcta taatagttac aatctttgaa aaatattatg caagaagcaa
     1381 tagtgatgca ggtttggtag tgatttgtga aaatactaca ggaaaaacac ttgttaaaat
     1441 tacctcaact ggtagtgcaa ttggactatt tcagattgat tggggaacag gtaataattt
     1501 gattaaaaga gtaaaaaaaa tacttattga ttatattatt taattatttt atgtagatat
     1561 tcttaggttg gtatggaatt tgtactaacc tattaagtaa tcaataaaac taaataaaca
     1621 tagaaagcac ttacaaatat gtaggtgctt tttattgaaa aaatagaaaa taaaataaaa
     1681 agtaagatat aggaaaaata tgtaagaatt atatgttata ataattgtag caagaagatg
     1741 taatctacaa tttatagagt ggagttcatg tattaaaaaa ttatcctccc aacgataaga
     1801 agggaggtga atatacatgg ataatttttt atttaatgtg ttagctagtg taacagctag
     1861 ttacatagtt tatttgatta gcaagttaat caaaaaagta aaaagccact ctaccgcaaa
     1921 gagtgactta aagattgata taaattttaa atatcatcgt aaaaaataac tttcagttat
     1981 agaacttcac tctaccgcaa atagattgta gttcttcttg tttttattat accacaaatt
     2041 agaaaaaata ttgcatataa tattttttat agtcaataaa aatatgaaat tttttataca
     2101 caatgatgat gaatctaact agatgaataa atttaattgt aaataagaaa gtacttactt
     2161 aaatggtagg tgcttttgtt ttgccaaatt atgctataat attgagtgag atgagtaatt
     2221 tgtgggggaa ttttataatg tggatattag taccaataat aacaatagta ttacttataa
     2281 tagctgtttc ttctatgcaa tatattttag ttatgatagc ttttttgttg attatatatt
     2341 cttttataga aaagaaaata gttatgggtc ttgtgtcagt tctttttttc acttattcaa
     2401 tttatctttg tgcaacttgg gaagataaat ccttgatagc agataataaa gttgagactg
     2461 ttagggcaca aagagaaact gtagaaagag aaaaagaaat ggaaagaaga agaatacaag
     2521 aagaagtaga caaagaaaga tatattgaaa aacatggaat ggaaatatca gaaaatgatt
     2581 taaaagttaa gctagaggct ttagtacctc aagaatataa ggggaagaaa tatgaattaa
     2641 aagtaggaaa atttaaaaga tatagtatgt attttgattt aactgtacaa aatgaaaaat
     2701 ttagtaattc tgaagaatgt aaaaaatttg taaaagaaat tgctaatgat cttaaaaaaa
     2761 ttaaaataag caaggcgtac tttaagtttc attcaaaaga tgacggcggt atatataatt
     2821 ctgtttatat tgattatttt agaaacatac aaaataatgt agataatgtt gaaaatttag
     2881 aattcaatga gtttgaactt aaaaccgaag aagaagagaa gcgagaacaa gagaaaatag
     2941 aacaagaaaa aaacagttat aataactaca ttcaaaacag agtggttgac ccactagata
     3001 gaataaagaa actaaaagag ttgctagatt caggagcaat tacacaggaa gaatataata
     3061 aaaagaaaaa agaattatta gaatagataa tatagtaagc acttacaagt atgtaggtgc
     3121 ttttaaattt acaaagtatt ccattttaat tttatagttt agattttatg atataataaa
     3181 aatatagaag ttttgcagtg tgcgatattt gttacaaagt agggcttaat acttgaaatc
     3241 taagatgttg agggtgcgtg ataagtgtta tcaattgcac tattgcccgc tcactgcaat
     3301 tttaagagta ttgtatatat gtaggtattg gaaatgctaa gtttattttg gggttttaga
     3361 ttaactatat ggaatgtaaa tattacatgg gtaaaaattt tgatgatttt tatgacagtt
     3421 ttagattaac tatatggaat gtaaattgta tagaattaag aattttttta atactaatta
     3481 tagttttaga ttaactatat ggaatgtaaa tattacattt aatttgcatt gctttgatga
     3541 cttctttgtt ttagattaac tatatggaat gtaaatattt ttcaatatca atatagaact
     3601 ttcatctttc ccgttttaga ttaactatat ggaatgtaaa tcaagatatt tttaataaaa
     3661 tttctgagcg aactgtttgt tttagattaa ctatatggaa tgtaaatgag gagattcaga
     3721 cagcgactaa aaagactgat ataaagtttt agattaacta tatggaatgt aaataacttt
     3781 atatctccat ttgcatatcc atctgatgaa cgttttagat taactatatg gaatgtaaat
     3841 gcgtacaagg ttgagaagct ccgcaatctc aacgtcgtgt tttagattaa ctatatggaa
     3901 tgtaaattct catgactatt gaataaatat aataaacaag catacgtttt agattaacta
     3961 tatggaatgt aaatttagaa tcaaaagcac ttaatcctag ttctttcata gttttagatt
     4021 aactatatgg aatgtaaata gctttttagc ttcgtctact actttataag caaagcgttt
     4081 tagattaact atatggaatg taaatgatga acaataaaac aatcatctaa agacgaagaa
     4141 agttttagat taactatatg gaatgtaaat gttcctgttg tgttttttac taatcccatt
     4201 ttatatgttt tagattaact atatggaatg taaattgctg tgatttcact aataaaacaa
     4261 ttaataattg tgttttagat taactatgtg gattcaaaat taaataaaca aagaaagcac
     4321 ttacaaatat gtaggtgctt ttattctgct caaaattggt cggttgggta aaatcattag
     4381 aaaaaattag taaaaacctc ttttctgtaa ctcgttacaa tattattatt aatgtaacga
     4441 gttacagaaa agaggtgaat aaaatagcaa ctaaaagtag ggcagagtat atgaaaaatt
     4501 gtcgaaaaga taaaagaggt tttagtgtac ttttagacaa agaaaagtta gataaatttg
     4561 atgaagtatt agaggaaaag aatctaacca agaaagaatg gctagaagaa aaaatcgacg
     4621 aggaactgga acaaaaggaa taaaaaataa gggtcactcc caccgaccaa agtttgagta
     4681 acccctatga tgtatactat cgtatatcaa ttataatata tgtcattcct taaaaaaatc
     4741 aattattaag gagtgtaata ttatgaaaaa tgaattaatg atgtttgaag gaaaagagat
     4801 tgaagtattt gaatttgaag gaagaatttt attcaactca aaagatgtag caaattgttt
     4861 agacataaaa aatgttaatg agaatataat acttatgaat gaaaagcaag ttgtaaagtt
     4921 aagaaattcg gatatcagta atactgacat ccgaaaactt aataatgctg gagaaaattt
     4981 tcttactgaa agtggagtgt ataaattaat ctttaaatca agaaaagaag aagctgagag
     5041 attccaagac tggataagcg atgaagtttt accaagcata agacaaactg gtgcatacat
     5101 aactaataat gtaatcccga aaagttgaga gaaaaagcaa gtgagattga aaaattacag
     5161 ttgcttataa cagtacatct atgttaaaag aattgctaga tgatgcaggc tttgacaata
     5221 aatcaaaact attaacagct aaaacattat ataagaaagc agaaattgat ttaccaatag
     5281 agataaacga agaagaacat tattttgata caaaacaaat agcatctaaa ctgaaaatat
     5341 attctaagag taataaacca gctcagatgg ctgtttgtga gattattaaa aagattgatt
     5401 tagaagatgg tgaagttaaa ggtgtttggg aaattaatgg ttcttggact ggtactgtaa
     5461 ataaatatac aaagagtgta atagataagg tgagaacttg gatagaggaa aataatagac
     5521 ctaccaagat tgcaggtgag aagaagaatt attatgtatt ttacaaaatt gagtaaattt
     5581 atcagttgta ttaaataatt tagtttagtt ttggggggat taatacaatg tgtgagaatt
     5641 tacttgataa tatgtatatt gaaaagagaa aagaagaata tagaattaaa cttttgaaaa
     5701 taagagaaac agatatagat atatataata agttagaaag tatagtatat aaactttctg
     5761 agaaaaaatt agagaaaaat aataaataga catataaagc acttggatat tctactgttt
     5821 caagtgcttg tttggtataa aatggtataa tggagataga gaattatatt aaccaaaaaa
     5881 tggagaaaat agagaagatg atttagttga cgctttatca gtaggagtac ctgcaggtaa
     5941 gacagaatct ctagtacgat tagttggaga tggaataagt gatactcaaa aaagtttaat
     6001 aaataaaaga gaatttagaa atataactca aattggtgga aatggaaatg agaaagcatt
     6061 tagtgaagta gaaaacttag ttaaatagat tagaaggtag caactagagt tggttgttac
     6121 cttctttttt tatactttct ctctctaagt attttatttt tcatatcttc taatacatct
     6181 aatgcagtag tataaaattg tatattattt ctataaggta tagctattgg aagaatgccc
     6241 ttgttaacta aaaaaataag aagtacaata aataaaagtg tcataatcat tctaataaaa
     6301 taattagtga tatctgataa atcaaatgtt aagttaattg ttgtagaaaa tacaacagcc
     6361 ataatagata tatacaaagc aaaaatgggg ttactatttt tttgtaactg tcctttaagc
     6421 ctagattgct ctatttctat atcttgaata ctttttatgg aatccatttc actataatac
     6481 ttataaactt ttaaataata ttttttatac ttgtttaaag cttcttcatt atctttaagg
     6541 tctttgtcac ttaactctga tttaacttcc ccaaaatcca taaaatccct cctacaatgt
     6601 tatataatat actttaattt atagcatatt taagaactta tatcagcaat ttgtcgaacg
     6661 attttagcta aaaatagagt ataatgatag gcttattatt tataagatat tattttttat
     6721 atagttatat atttctttct aattagataa tataatgctg ttgttataat ggctactaat
     6781 attattgcta ttattgtaat atctaatctt cttgtaaata agcctattgc tatatactca
     6841 agacctgtta catagcgata aagtacttct agtttaacag tttttttctt atctatattt
     6901 ccatatattg attctaattt attaaaatat tttttactac aaaaagcagt tttaggttta
     6961 attaaatata ttattgtagt tcctattata actattattc caataagtat aagattgata
     7021 tttccaaaat ccatagcaga tacctcctat taaatttgct tttcttaatc aaagtatatc
     7081 agtaacacaa gtacaaagag tatctaaaat attacaaata ttaacaggat agataaaact
     7141 atagtaaaaa ttgtaataat aaagcgaacg aaaattatca tactaaaaca taagacatgt
     7201 tataattgta ttagatagaa gattaacata caccaaagca ctccttataa taaagagtgc
     7261 ttattttttt gaaattcatc aatatataaa ctatcaagaa ccttattcaa cacaccttaa
     7321 aatcgattta aatagttctt tttgataaca taatttatat gatataataa aaaaatagaa
     7381 gtgtaccacc aatacacttc tatagctata actaagcatt ctcttgctgg gggagtgctt
     7441 ttttcatttc tccaaacaac attatttact tcatcagtaa ttatcaaatc aatctcatta
     7501 tctttcaaaa tctctgttgc agtacaaatt agattatcta agtcaaaact tcgagttatc
     7561 tctagttgtt tgttatttac ttctcttttt agtatataag ttttaaaata catttatatc
     7621 accccttgta tttactcttt tttctctaaa aatatattga taagtaaata tgcaacaaat
     7681 aaaaaaatct caatgacaca tagtgttata gctataacta gaagtgattg atttttccat
     7741 gcactataag ctatgatgaa aagaaatgta aaagtaaata atgtataatt cataatttta
     7801 actaagatat gacttgtttt cattgttaag tacaagtctc tttcatctgt actatcgtgt
     7861 aattcttcaa caatgccttt tcttgaaaaa gcattacaaa aattaaagat tgctattaca
     7921 attaaaagaa atgatgaaat taggaatctt ttatctttta aaatgcaata aataactaaa
     7981 ctagcacaac ataacacaca aactataata cccactataa aacttcgttt actctttact
     8041 ttcatgtttt ttatcctcca tttctttatt ttcttttaaa caacatagtt cttcaatcgt
     8101 tgtaccaaac acttgtgcca tacgataggc aagcataaga gaagggctat attgctcttt
     8161 ttcaatagaa ataattgttc tgtttgatac atggactaaa tcagcgagtt gttgctgtgt
     8221 tagacccata tttgttcgaa attctcgtac ttttgttttc attccataac ctctttctat
     8281 atgaagctaa cttcatgtga agttaacttc ataatatcct aaaatgagta aaatgtcaat
     8341 ctcattagat aagtattata aaacctaaaa gaataatatg gtaaaaattg gatggaatta
     8401 tttaccttaa ttctcttgtt tgtaaaactt tattgatata gaatttaaca tatacataaa
     8461 tcggcaaagg ggcgcgaatt atgaaaatta gtaaattacc agaagcggaa ttaaaagtta
     8521 tgagatatat ttgtgaagct aacaaggtat taacttctag ggaattagtt gctgcaatgg
     8581 aagaaaaata tgagtggaat gaatcaacta catttacagt tttgaaaaga ttagagcgaa
     8641 gagagttttt aagtacagag aaaataggca aacttacaca ttataatata ctagtaaaag
     8701 agaaaaaata tctaaggttt gaaacaaagg aatttttgaa gaacatacat aaaaattcaa
     8761 tttcaagttt aatgtcagcg ttacatggtg aagacgacga agtggatgaa gataaattaa
     8821 gtgaactcga agaatgtttt aagaatttga aataagaaaa tgacaaattg atacataata
     8881 aataaaatga aggtatgaaa ttataacttt ttctaccttc attttgttta ttattctgaa
     8941 catgttaaga aaatgaaata agtttgtatt ttagttatat aaattttaat agaatatgta
     9001 cagtatatag tagatagaat gtataaaaat agaatttaaa taagtagcta ttattgcagt
     9061 gaaatgaatg tttctaagta tatttgggtg gaagaatggt ggaagaaaat tagtgaaatg
     9121 taattcttcc accattcttc cacctagaaa atagaaactg tttaataaga attaatatac
     9181 attaattcag ctgaatgcaa tcaaaatact taaatgaaag taatctgaaa acaacataat
     9241 tcaaatcaat ttattcaaat aaacctcaat ttatcttaaa tcttaagaaa tatttaagaa
     9301 ttaggataat attttcttaa gatttatatt ttattatata gttataaggg aaagttacaa
     9361 aaatgtaagc caaagtaata taacaattta gtcattgttg agaaagggag tgaaagatat
     9421 tgtataatat agataagtat tattatacaa tagaggaagt gaaaatatgt acaatatatt
     9481 agtagtagac gatgataaag aaattgttga ttcgatagaa atatatctta aatcagaagg
     9541 atttaaaata tataaagcat atgatggaat gaaagctttg gaaataataa cagagaatga
     9601 cattcatcta atactaatgg atataatgat gcctaaatta gatggaatca aagcaactgt
     9661 aaaaatacga gaagaaagaa atattccaat aattttaatt tcagcaaaaa gtgaagatac
     9721 agataaaata ataggattaa atattggagc tgatgattat ataacaaagc catttaatct
     9781 attagagctt atagctaggg ttaaatcaaa cttaagacga tatgtaactt taggtaatta
     9841 tgaagataca tgtaaagaaa ttctaaaaag tggagctttg gagttaaata cactaacaaa
     9901 agaagttaaa attgatggtg aaaacataaa aatgactcca attgagtata aaataattga
     9961 acttctttta gaaaataaag gacgagtttt ctcaatagat gagatatacg aaaaagtatg
    10021 gaatgaggaa agttttaatt cagaaaatac agtagcagta catatcagac gaataagaga
    10081 aaagattgaa ataaatccaa aagaaccaag atttttaaag gtggtgtggg gagttggtta
    10141 taagattgaa aaattttagt ggaaaaatta taaaagaaaa gagtttaata tttaattgca
    10201 ttttgatttt agtcattttt acatttacac aaatgttagg aattttaata tgttttaatt
    10261 ttttctctga gagtttaatt tactctatgg ggattatgct tttcttaaac tttattttat
    10321 ataaaatact gacaatgtag atgaggtggg caacttggag aaatatagag tattaaagtt
    10381 agtttcatta atcataatat ctatctcatt tccattaact atattgttgg tgacaatagg
    10441 acttgctgag cctaaataca atatacaaaa aacttctaca aagagtggga tttttgatat
    10501 atctgatgat ttttatgata gtaatatatt tgacgaagga acaatggcaa gtctattacc
    10561 caagtttaag ctatctgtag gtggtaaatt aactagtaaa gaaaaaggta aattccaaca
    10621 agaaggtaaa agtatagatt taagtagtag tgattctgag tatgataaaa tcatgattaa
    10681 tgaacaagct tctgaagaat atttaagtta tctaaaaaat ataaaagtgt atgttattaa
    10741 tacatacaca ggagtatttt atacaaatac gtcatatgct agtttatatg attttaaaca
    10801 aaaaactaag agctattgta atgtagagat aataaataag ataggaaaga gctcatatat
    10861 taaaacaata aatggagaaa aatttgaact gaataatctg agtctggaac taaatgaagt
    10921 agatgaaggt tttgaggcat atgtatcttt tccagaagag ccaactatag aagatggaat
    10981 agtatacact aattttcaaa tatttaaaca agctacagaa aaagtaagat tattttttgt
    11041 agctagttta agtttagcaa ttatatgttt attatctata attttatata tcaaaataaa
    11101 aggtgaagtt ttaaaaagaa ataatgtatt tttactaata tacagtaaga tacctatgga
    11161 atttaatgta atattagtat ctatttcaat tgcaatttta actatatatg ctcttgctgt
    11221 atcttatcat attgatgtaa ttgcattagt ttctttatct ttactgatag tatcaaataa
    11281 tatatttttt ataaatgtgc aattgagcta tttagaaaat aaaatgtgta tatttaagac
    11341 aagtattata gtaagagtga ttttatggtt aataaaaact gtaagggagg tcagtaacgc
    11401 tataaagaaa gtatctttag caaggaaagt tattgttata gtgataatat ctttagcttt
    11461 aatcatggca attagcactc gaagtatagg tatgggatta ctgttttcta tatgtatttt
    11521 tttaggattt acaatatata taactaaaag actttcatat cttagttacg taatagaagg
    11581 tacagaaaga ataaaaaagg gagaattaga ttataagata aaaattactg ggaatgataa
    11641 ttttacgtct ttagctgaaa atataaataa tattggagaa ggtcttgata gagctataga
    11701 agagcaagta aaaagtgaaa gaatgaagtc tgaccttata acaaatgtat ctcatgattt
    11761 gaaaactcca cttacttcta taataaatta tgtagattta attaaaaaag aagaatcaat
    11821 acaaccagag tatataaatg attatataaa tgtacttgag tcaaagtcaa aaagactaaa
    11881 attattgata gaagatttat ttgaagcgag tagagtaagt agtggcaata tagaattaca
    11941 aattagcaag attgatttag tgcaactatt aagacaaagt ataggtgaac ttgaagaaaa
    12001 attatctaaa aataatttat atttaaaatt aaatgttcca aatgataagg tgtatatatg
    12061 ggcagatgga agaagaatgt atagagtatt tgaaaatctt ttatctaata tagcaaaata
    12121 ttccttagaa gaaactagag tctatattga tgtatatgac tatggtgaaa atgtaaaggt
    12181 aactatgaaa aatatatctt cttatgaatt aaattttgac ccaagtgaga ttatggaaag
    12241 atttaaaaga gctgatgaat caaggaatac tgaaggaagt ggattagggc ttgctattgc
    12301 aagagattta gtagctttac aaggtggaaa attttatata gaaatagatg gagatttatt
    12361 taaagcaaat ttagagtttc aaaaatatga agaagaaata acaaatatgg aatgagatag
    12421 tatttaagta tatatatttt tttactgtcg aaagctttta ttataatata ttagaatatt
    12481 tacagtagag aatgaagagt aaaaacagag taactttaag tcactctgtt ttttatttaa
    12541 aatcaataat ataacaactt aaattaaaat tgtacacata gttttaaaat aacttataca
    12601 ataaaacatt atccaaatat cacaagataa ttccaaataa atatatagat atcactcctg
    12661 taataataga aataccaata gatttaaact tatatgcaac aagaagtaca atcataaaag
    12721 atataatttt agggttagct atagataaat atagactatc tccattaaaa aatacatctt
    12781 tagcagttaa ggaagtaagt attgctacag gtataaattt catgattttt ttcaaaaaat
    12841 caggcatctc tttttttgaa aaaattaaca taggaagcac tcttggtaga tatgtaacaa
    12901 tgtacattcc aattataata aaaaatatat aattattatt cataagattc ctccttttta
    12961 cagtaagatt cttctataaa gaaaccaatt aaagatgcta aaattgatgc aactattatt
    13021 actaaattgc ttttgaataa tgcatataaa ataacagaaa taatgattga tgatataaat
    13081 actacaatat aaatcttatt ttttatttga gaaactatta aagctataaa catagaagtc
    13141 aatatgaagc cagaaataga actgtctata gatatacttt ctcctaaaaa tgctccaata
    13201 aaattagcta aaactgatga aattaagcag aagaaattaa gataaatagc atttctatca
    13261 gaccaatttc cagcagtaaa tttttcataa ttaactgcaa aagtttcatc tgttatagta
    13321 tgacaaaatg gcaatataaa ttttggactt ttatttttta tgtacatgtt caaattagaa
    13381 ctcattagaa aatgtcttaa gttaactata aatgtagtca aaacaataga tgttacactt
    13441 gcaccagtag aaatcattga agctgcaatg aattgagaac ttcctccaaa tactaaagtt
    13501 accattagtg ctatttgaaa aggtgaaaga ccagattttt gtaatagtat tcctcctact
    13561 atacctatag gtatataacc taagaatata ggaattgaag caataaagct ttcttttaat
    13621 tgttgagttc tttccacaat aaatcactcc ttttgtatta aatgtattaa agtattcgta
    13681 aaacagaaaa atagattaat tgtataattg caattataat taaaaataaa tttggattaa
    13741 tatataaaat actatacaac aaatagataa tcttaaacgg ttattaaatt ttatctattt
    13801 gttattatga tgtaataata catattaatc tctataagtt aattactatc tggtgtaatt
    13861 ttattttaac aacaaaaaaa aatacacaca attaaaatat tgtgtgcaat acaattattt
    13921 tttctcttct aatagtcttt ttaatacgct caaaccagct ttaagttctt ctgtactttc
    13981 tgtagcacat acagctattc ttattgcaga aattggtttt tcttttccaa cagcaaaacg
    14041 ttcagaagca tagacctgta caccttcttt aagtgcttgt atctcaaatt tttctgccat
    14101 tattccatca ggtagaatca accatctaaa tatacattct tcatttccca aaagattata
    14161 tccagataaa tattgattaa taatcttgtt tcttcttctt gcagatttac ggcgtgcatt
    14221 aattaattta tcggcttcac ctgatgtaat catgcgataa gcaatttctg ttaaaaaata
    14281 agattgagat agattaatgt tgtataatgc gcttgataat gcctctcgat actgtttagg
    14341 tgtagagata tatgctagcc gtaaactagg tgcaataatt tttgataaac tcgtaatata
    14401 aattgtttga ttgggcagat aggaagccac tggatttaaa tgtgtttcat taagtaaact
    14461 atgaatagca tcctcaataa caattaaatt gtatttagat gcaatatttg caatcatttt
    14521 tcgtccattt tgtgacatta tatgtgtagt tggattttga taatctggta ttatgtatag
    14581 acctttaata ttttcatttt tacaagcata taatagtcct tcttcactaa tctcattatc
    14641 ttcttgttta attggaatta gttgaatccc aagcatttta gctgctgtct ttattccagg
    14701 ataagttaat gggtctacac ctattctatc tccatgttga aacaaaccag ctaaaatagc
    14761 aacaattgca ttttgtccac cacttgcagg aagtaagcta tctgatgtag tttcaagccc
    14821 agctttataa attaatttgg caatagcttc tttttgccat ttagcaccat ctctttgtcc
    14881 atattggaat atggttttaa aatctatttc cttaattatg tgtttaaaca aagtattcat
    14941 ttcttctagt gtgaaatctg gattcattgt gcccatttca ataaatgtaa ttttattatt
    15001 actggacatc aagaatagat ttgaacgtgt atcataagat acaaaagttc cacttcctgt
    15061 aactccactt attagacctt ttttttcaca aattttaaaa gctctagaga tagtactcac
    15121 attaacatct aagaaatctg ccaattcacg ttggggtggt aatttagtac ctggtaataa
    15181 aaatccattg ttgatatctt gttctagttg ttctgcaagc gccttatata atatttgccc
    15241 ttttgaaggt ctctttggtt tccagttcat aggataattt tcaaaagaat taattggcat
    15301 aagttttcac ctcacattat taatttgatt ttttctttat ctaaaacaat aaattctaaa
    15361 gatacaaata aaaactgata ggaaataatt ctatcagttt ttgtctttta attgatttca
    15421 aatcttccta taatttttat accttcttta tcataaagag ggattgtttt tttaaatata
    15481 ttaaaatttc tttttcttgt atataaaaca gcttcttctg gtgtctttgg ttgattatca
    15541 caatcgtaca aatctctttt ttttacgtag ccgacttttt tacctctacc tattgtttgt
    15601 ataagttctg gttcaatgga atttccattt gagtctatta tatgtgtacc ataggtttct
    15661 ccatttttat tataattaat cttaccaata gtatattcta catcttcatt ttgtttaaaa
    15721 ttggagttga tagaaaatat tgaccctatt attataaagg ttaataaaaa cttaagacct
    15781 aaattaattt tctttttatc ttgattaaac atagaatcta acctcttttt taaaataata
    15841 ttttttttat ttagaaactg agatacgaat gatgaatact tcaaagattt ttgatacttt
    15901 attgtatcta atattatata tgcatactct ttaatttcac tcgtggtaca cttgtcaatc
    15961 acaatttcat cacaagataa ttcacataat tcatgaaaat atttttttaa taagtataaa
    16021 attggattga accaatatat gatacaaatt atactaatta gaagcttaaa taagttatct
    16081 tttcttttat aatgaattaa ttcatgttta aagatgaatc ttaactcttt attattgtac
    16141 tttttttctg gaattattat tttacagcgt gttaaaccta taatggcagg agaataaata
    16201 cctttaacct tatatatctg aagtttttta tttatactta gttcttttgc ttgttcatct
    16261 aagatactag taatatctat ttctaaaatt tcttttttaa aattttctat gtaattataa
    16321 aatttaaaat ttgttcttaa atagtaaaaa gagattgcta taattcctaa tatccagatg
    16381 tacataaata tatctaaagt aatgccctgg tcatatggta tataattatc gatattatgc
    16441 ttatagatat taatagtagt attattgata tttaatatat ttaaatttat tttagatata
    16501 aacatcattc tgactactat taaaagacat ataaaataat taaacttaaa agtatatttg
    16561 cttatctttt tagtaaataa aatgaaaaaa agaattagtg gacttatgta aatattaaat
    16621 ttaataactt ctataaataa ttccataaaa tacgtctcct attttctttt ttttagccat
    16681 tgttcaagtt cttttatgtc atcatctgat atttcatttc cacaacctaa tgatgatata
    16741 aagctatgaa tagaattttt atgtataagc tctaaaaaat cctttgtttc aaattttaga
    16801 tattcctcat gtttaacttt aatagtgtat aaagtatttc taccatcttt ttcaaattct
    16861 aaaaaacctt tatcacttaa tctagaaagt actttaccag tagtaccttt tgtccatcca
    16921 tgagaaattt ccatataatt agctacttct ctggctggta ttttatctat gtctttatcc
    16981 catatgaaat tcataactaa caactcggat tttggtattt ttttcaatat aatcactcac
    17041 ctttaacttg attatattag acaaatgtct aatagtcaat ataatatatc atcatgtata
    17101 aataatcttt atattatttt atgcttgaga actaagtagt atagatttat agataaaaaa
    17161 gatattgaga gttttatgga agaggggaat atattttcta ttagagataa gaatatgaca
    17221 tatatttttt attgattttt acaaaaatac tatatagtga ataaattaat atcttttcta
    17281 aattagaaga ggagaatctt attagataaa gattatcctc tttattgaaa gtttaaggga
    17341 tattagagat tattaatttt tctctctttt tttaactaaa agcaataata cataaaatta
    17401 ttattgctaa tgatactctt aatgtggtat atagtcatag tgtcacatgt ttatcaatag
    17461 ttttttcatt aattattgta acgaatagta ggactcataa aatacttaga aatatgtttt
    17521 tagatagatt ttaaatatat aatctaactt atatctataa attaacacta ttattctcca
    17581 attccatttc tagttttttt ataaatttat taaattcttc tggttgcctt tttccaaaca
    17641 atactcttac aagagctgaa ttgatttttc caaatatatt cctcttgctc tcactttcaa
    17701 taaaaataag ttttgtattt tctaagtcaa tatgtactaa ttcatatcta gtaatagtac
    17761 tttctctttt attgcttgtt tttatctcat atactctatt tttttcgaaa ttagttattt
    17821 caaccttaaa tcttttatta tttttttgct gtacaaatgt tcctactgct tttttctcat
    17881 taaaattagg aaaatttagt cttgcagatt ttgtaaatat attaaatatg tcttttgcag
    17941 aatattttaa taaaacttca gttttaaatg tattcatatc gttttccctt ctgta
//
LOCUS       DAMJYR010000075        17778 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.075, whole genome shotgun sequence.
ACCESSION   DAMJYR010000075 DAMJYR010000000
VERSION     DAMJYR010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 17778)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17778)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17778
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.075"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            48..383
                     /locus_tag="PC471_002441"
     CDS             48..383
                     /locus_tag="PC471_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2789565.1"
                     /translation="MYNIDDMIYNCPVEALSNILGKKWVAIIIWKIQEDKKRFGELQR
                     AIPDCSKKMLVQQLDFLIEQGIIVNQKKLVNNVVESTYFLSESGLRLLPVMEKMIMWS
                     NKNLNCDKY"
     gene            520..1797
                     /locus_tag="PC471_002442"
     CDS             520..1797
                     /locus_tag="PC471_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin family protein"
                     /protein_id="HDF2789566.1"
                     /translation="MQNVNLFLVFIEGIVSFFSPCILPILPIYLSILSNSSVENLKEG
                     KTSFIGSSLFKNTIFFALGISTTFFILGSSVKVLSMFFNENKDLIMFIGGIIIIIMGL
                     FYMGIIKSSILNREKRFNVKFKEMKAITAFILGFTFSFGWTPCIGPILASVLVMVSSS
                     SNHLSANLLIAVYTIGFILPFIITAMFYSKLFKTIDKIKSNMEIIKKIGGIILIVSGI
                     LMMVNGFGSISKHFNTSQNSKIESKQEENKRENSTDKEENSDGNDSQKDSNNDNNDKG
                     SNDEDRIKSIDFTLTDQYGKTHKLSDYEGKVVFLNFWATWCPPCKEEMPYIEQLYKDY
                     NKNNDDVVILGVASPNLGREGSREHVVNFLKDQGYTFPVVLDEDGALAYQYGINAFPT
                     TFIIDKEGYVTQYIPGAMDKATMASFIENQRNK"
     gene            1918..3306
                     /locus_tag="PC471_002443"
     CDS             1918..3306
                     /locus_tag="PC471_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HDF2789567.1"
                     /translation="MKKTFDAIIIGFGKGGKTLAGDLANRGLKVALIEKSNKMYGGTC
                     VNVACIPTKSLENSANSVKTKNINSWDEVQAEYEKAIDKKETLITKLREANYNKLNSN
                     ENVTIFTGMGTFIDEKTVQVKTENEIYELVADNIFINTGSRPFIPNIKGIENKNIVYD
                     SESLMNLRTLPKKMTIIGAGFIGLEFAGIYSSFGAEVTILNSNNGILPNEDVEDSEEI
                     IKLLAKRNVKIVNNANIKEIKEVSELAIVEYEVDGKSKELTSNMILVATGRKANTEEL
                     GLENAGIELNERGFIKVSETLKTNKEHIWAIGDINGGPQFTYISLDDYRIVINQLFGD
                     KTRTTNDRKNIPNSIFISPAFSRVGLNVKQAKEKGYEVLVAKMPVEAIPRAKQIGKAD
                     GFIKIVIDKKSNKILGASMICENSSEIIHLIQLAVDLEVEYTYLRDRVYAHPTMTEAL
                     NDILSPNMIKEV"
     gene            3677..4390
                     /locus_tag="PC471_002444"
     CDS             3677..4390
                     /locus_tag="PC471_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017208807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF434 domain-containing protein"
                     /protein_id="HDF2789568.1"
                     /translation="MNKISKRGFDDSDKRWFSSKELTRLIKSKEEIEWLLNRDYKIDP
                     VVTFVGDRYQFSIRQRDALKRAVCTDEKNIIRQSKRLSLDKIKEDTVNIDGFNLIISI
                     EVALSGGTLVIGSDGNIRDLAGLRGTYKIIDKTEEALNLIGKFFNKYNAQNLKFYLDA
                     PVSNSGNLKYRILEHAKTWGIETEVELVKNADVVLEKLDRVVSSDAVIVDKCISYFNV
                     ARGIIEEYIKECNIINLNK"
     gene            4508..5377
                     /locus_tag="PC471_002445"
     CDS             4508..5377
                     /locus_tag="PC471_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HDF2789569.1"
                     /translation="MRGWICVKLTKLNIKKYRAPIYKYALANVNLRSAKSTNSSIITV
                     IPQGAKMEVLDEEDDWIKVMYNSQEGYVYKDLVSVSEYAWSNLNLREDKSTTSNIITV
                     IPEKSRVEVLQVDGDWSKVVYDDKIGYVFNYFLSIDGNKPNELDYKYFYTDMIKFVNE
                     NNIKSTSDYLIVTDLRNKYTYIFKKDNGGWGQLYKWQCTIGKPETPTITGIFYISGRK
                     PSFGTDEYSVKYATRIKGGYYYHSVLYDSTGSYIIDGRLGEALSHGCIRLSTENAKWI
                     YDNIPDTTTVIIH"
     gene            complement(5532..6677)
                     /locus_tag="PC471_002446"
     CDS             complement(5532..6677)
                     /locus_tag="PC471_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HDF2789570.1"
                     /translation="MSIYYVPPINLLGKGCLNEAKDSIKSLGTKKAFIVSDKFLVSNG
                     TVKKVTDILSQIDVDFVVYDEVKQNPTVTNVNKGLKILKSENCDFVITIGGGSPQDCG
                     KAISILATNGGDIRDYEGINKTSKKCLPIIAITTTAGTSAEVTINYVITDEDRHVKMI
                     MVDTNSLATMTVNDPELMISKPSNLTAATGMDALTHAIEAVVANGAYDVTDSTALYAI
                     KQIFKYLPRAVKNGNDIEAREQMCYACFLNGIAFSNAGLGNVHAMAHQLGGLYDLPHG
                     VCNAMLLPIVEEENAKSAPAKFRPIAEVIGMDVNNKSDKECVDFVIDKIKALSEEVGI
                     PKSLKDVGVDNPNFELLAENSMKDACAGANPVFFDKDKIIELFMKIS"
     gene            complement(7010..7786)
                     /locus_tag="PC471_002447"
     CDS             complement(7010..7786)
                     /locus_tag="PC471_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HDF2789571.1"
                     /translation="MTSDNHRPTARILDILEALAESENGYTLTEIADVINAPKSSIFP
                     IVHTLNARKYITIDKNTSKYSIGIRSYTLGSSFFEKRTIFNYIIDEMTNIVNSCSETC
                     QLGILDNNEVLYIGKVDSPEPIRLISYVGKKIPANCTGLGKALLCDFDKEKLISLYPD
                     GLKGFTEKSITDFDVLYDQLLEVQRTQIASECEESMEHLKCLAVPIRKNGCIRAAVSV
                     TLPLFRANDEKIELIKKLLLNAQKNIQSMMEERNEDIVIN"
     gene            8368..9342
                     /locus_tag="PC471_002448"
     CDS             8368..9342
                     /locus_tag="PC471_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate permease"
                     /protein_id="HDF2789572.1"
                     /translation="MIMDFIKKIPAGMMIVPMFIAAFINTFCPDIVQIGSFTTAVFSS
                     VGSATIIGVQLVCLGTQLQFREMPKVLKRGGILLGSKFAIGALIGILIGKLFGMDGVL
                     GLTTLAVISAVTNSNGSIYLSLMTSYGDETDCATMSLLTLNDGPFFTLIALGTSGLAN
                     VPLLSLLAAIVPILVGMIIGNFDKKMKEFLEPGCNLLVPFVGFALGSSINLASILKGG
                     LSGVLLGLISVFVGGIFIVFCDKFIGKRPGYAGWAVATTAGNAIAVPAAVALVDPSWQ
                     PYVATATTQVAAAVVVTAILVPLITSWWAKKYGCPKFPKQNEDGTITA"
     gene            9440..9745
                     /locus_tag="PC471_002449"
     CDS             9440..9745
                     /locus_tag="PC471_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HDF2789573.1"
                     /translation="MINAVIIDEKDNVAVAIEAIAKGSEISFKLKDKTVKTITALDDI
                     TIYHKLAIKDMAQGDKVTKYGEHIGEANQEIKSGQHVHIHNVRSVREDLDKQVMTNI"
     gene            9901..11061
                     /locus_tag="PC471_002450"
     CDS             9901..11061
                     /locus_tag="PC471_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HDF2789574.1"
                     /translation="MKFYGYKRPDGRVGIRNHILILPTSVCASDTTRIIASQVQGAVT
                     FNNQNGCSQVHSDQQLTMDVMAGYAANPNVYGVIVVSLGCENCQNDLVVDAIKERTNK
                     PIKTLVIQEEHGTLKTIEKAVRYAREMAQEASLLRKEEFPISELILGTECGGSDPTSG
                     LAANPLIGELSDKLVDLGATSILSETTEFIGAEHILARRAVNEEVKEKILHIVHRYEN
                     SLKLVGEEVREGNPSPGNIAGGLTSLEEKSLGCIHKGGHRQISEVYDYAKQIDKKGLV
                     IMDTPGNDASSVAGMVAGGAQIVVFSTGRGTPSGNPIAPVIKITGNKITFANMEDNLD
                     FDASPVIYGPQTMEELTDDLLNMVVDVANGKQSKAESLGYTEMAIARVCNYV"
     gene            11111..12013
                     /locus_tag="PC471_002451"
     CDS             11111..12013
                     /locus_tag="PC471_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HDF2789575.1"
                     /translation="MKFVSFTEGENECKRTGVFSKDELFIIDVNSLNLSRNFKDLNEL
                     IQNVSSEDIIKLKTIINESTFYNYDVFKRERCTVHVPIEKPIHDILCVGVNYKDHLEE
                     TQTHFDDSFEEPQKTVYFTKRVTKAIGPEDEIDGHFEINNQLDYEVELGVVIGKTGVN
                     IRKEEVEDYIFGYTVINDISARKLQKEHVQWFRGKGLDTFTSLGPCILHKSAVPFPIK
                     LKVCSRVNGEERQSSNTGLFISDVSDIVSELSKGMTLEAGDIIATGTPSGVGMGFSPP
                     RYMSSGDVVECEIESIGILKNYIK"
     gene            12093..12986
                     /locus_tag="PC471_002452"
     CDS             12093..12986
                     /locus_tag="PC471_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HDF2789576.1"
                     /translation="MKAEFLTPVVTIFDEEGNLDKEGNKKVYDHLIDGGVDGLVIMGS
                     TGEFFNMSMDMQKELIDLVTSHVNKRVKVFIGTSRMCINESIELSNYAHNSGADGVMI
                     ISPYYFSLDDDSIELFYSEIAKNTEAEIYLYNYPDRTGYDLSPELTLRLARKHKNIVG
                     YKDTVTLMGHTRDLINTMKIEFPEFKVYSGYDENFIHNLMSGGAGCIGGLSNLIPEVC
                     SKWAEAYNNRDMEKVIEIQKYINKAMDFYLITNPFIPAMKKAMNIRGLQISDYMTAPF
                     VKPNEKQVCEIKKLMNELNIC"
     regulatory      13039..13220
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            13448..14578
                     /locus_tag="PC471_002453"
     CDS             13448..14578
                     /locus_tag="PC471_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDF2789577.1"
                     /translation="MKFKSNVLKQFFLIAMVIFMVGGLIGCSKSDKENKQASTGKKLE
                     LKYASGFSVEDLEDGIKKVTDGDNRELILIPKKIKIPEKYKNANIVRTPVDNVLIAST
                     TQACSLRTIDELDSIKAVTTQEQYWTIKEIKDLMKDGKIHYVGSNLAPDYEKIVDLNP
                     DLTIVSSGLSEADTKFIDKLKELGLNYVVDNEYKEQNPFGRMEWTSLIAAFYDKEDMA
                     TSELNKTVQKVEEVSKKIKNKAKPKVVWASVYEGSAYIAPKDSYVDNMIKMAGGINAC
                     ASIDSNEGRVSIEQLHEVAKDADIFVYSSSSDYAPNLDSIKSSAPVLADLDVVKNGNL
                     WVFAPDYYQSVDKTDELIVDLVEMFHPGTTGEKIKHYINYDK"
     gene            14650..15675
                     /locus_tag="PC471_002454"
     CDS             14650..15675
                     /locus_tag="PC471_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HDF2789578.1"
                     /translation="MDFINKMRIRNTTIFIVLIISMILTFLVCVAFGSVKIEFKEVID
                     VVLRGMDNTEHYKIIYNIRIPRVLATMVGGACLAVSGILLQIFFKNPIVEPYILGVSS
                     GSTLSVALMILGGYTLGFESVNSMSIFVAAFIGALIVMSIVILFANKVKNITTLLIIG
                     IMTGYVCSGITNVLQSFANSDKLKEFTMWSMGSFSGFTWEKVEILIIVGILGLLMTVF
                     IIKPLNAFLLGEEYAKSIGINIRFFRVLIVFISSILAAIVSAFAGPIGFIGLAVPQIS
                     KMIFKTSDNRVLIPVVIFLGSIVTALCDLISRVILSPVELPISTITSLLGAPIVVMLL
                     VKRKDRA"
     gene            15678..16874
                     /locus_tag="PC471_002455"
     CDS             15678..16874
                     /locus_tag="PC471_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2789579.1"
                     /translation="MLKTNNLSVGYNNKVVISNINVEVKNGEILCLLGSNGAGKTTLL
                     RSLSKLISPIKGEIYLNGVNINCISRKALSKKMALVLTNRLLGDLMTVQDIVNIGRYP
                     YTGFFGSLSKKDLIMVDEALESVDALHLKKRYFDELSDGEKQKVLVARALVQEPEIII
                     LDEPTTHLDIKHRLELINILKKLSKEKSISVILSLHEIDIALKSCDKVALIKNNKVIA
                     YGQPEDVVDEDIINSLYELDDKNFNSLLGSVEISNKSKNEVFIIGGGGKATPIYRAFT
                     KKGIGLYSGIIHENDIDYEIGRTMGIKMFTENPFEPISDESFDLAIRNLNDSKIIIDT
                     GFSVGETNKRNIDIIKEALKLDKKVYSFRNRDESKKYYDSLDSKIEHIDKVSQIINSA
                     DINNLL"
     gene            complement(17002..17727)
                     /locus_tag="PC471_002456"
     CDS             complement(17002..17727)
                     /locus_tag="PC471_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HDF2789580.1"
                     /translation="MKTISKQELEKTLEKHYLWLKNNNAGEKADLKELRLENMDLRGY
                     CLSNIDFSWSDLINLNLEKADLENSIFNNSYLSDCSLKNSILKNADLSGASFRFCDLS
                     NSDIRGANLENSNLEYATLNGVISDESTRFFKLYCPETGAFIGYKKCFNFRMVQLLIP
                     SDAKRVSATSNACRCDKAKVLSITSVDGKESFKSARAYADENFIYRVGETIVAEDFNE
                     NRWKESTTGIHFFLTREEAIGYL"
BASE COUNT         6825 a         1983 c         3091 g         5879 t
ORIGIN      
        1 tataaaagtt tgtttaataa attaaataaa gaaaagagga gaataaaatg tacaacatag
       61 atgatatgat atataattgt ccagtagaag ctttatcaaa tattttaggt aaaaaatggg
      121 tggctataat tatatggaaa atacaagaag ataaaaaacg atttggagag ctacaaagag
      181 ctataccaga ctgtagtaag aaaatgttag ttcaacaact agatttttta attgaacagg
      241 ggattatagt caaccaaaaa aagttggtta ataatgttgt agagtcaaca tattttttaa
      301 gtgaatcagg attacgattg cttcctgtca tggaaaaaat gattatgtgg agcaataaaa
      361 atttaaattg tgataaatac taaaatttac tctgtaagta atgaaaattt aattaagcta
      421 tgagtagaaa aatgtattaa aattacataa ttttttagtt agattttaat ttagaaatgg
      481 gattgtaata ttggaaataa aataaggagg aattttaatt tgcaaaatgt aaatttattt
      541 ttagtattta tagaaggtat agtatcattc ttttcaccat gtatactgcc aattttgcct
      601 atttatttga gtatattgtc aaatagtagt gtagaaaatt taaaagaagg aaagacgagt
      661 tttataggaa gttctttatt taaaaatact atattttttg ctcttggaat ctcaacaaca
      721 ttctttatat tgggttcatc agtaaaagta ttgagtatgt tttttaatga aaataaagat
      781 ttaataatgt ttattggtgg gattataatt attatcatgg gattatttta catgggaata
      841 attaaatctt caatattgaa tagagaaaaa agatttaatg ttaagtttaa agaaatgaaa
      901 gcaataacag cttttatatt aggttttaca tttagttttg gatggacacc ttgtattggt
      961 ccaatattgg catctgtatt agtcatggtt tcaagttcaa gtaatcatct tagtgcgaat
     1021 cttttaatag cagtatatac tattggtttt attttgccat tcataattac agctatgttt
     1081 tacagtaaac tatttaaaac tatagataag ataaaatcta acatggaaat tattaaaaaa
     1141 ataggtggga ttatacttat agtatcagga atattaatga tggtaaatgg atttggaagt
     1201 ataagcaaac actttaatac gtcacaaaat agtaaaattg aaagcaagca agaagaaaat
     1261 aagcgagaaa attctactga taaagaagaa aattctgatg gtaatgatag tcaaaaagac
     1321 tctaataatg acaataatga taaagggtct aatgatgaag atagaataaa gtctattgat
     1381 tttactttaa cagaccagta tggtaaaaca cataaattaa gtgattatga aggcaaagtt
     1441 gtattcttaa atttttgggc tacatggtgt cctccatgta aagaagaaat gccttatata
     1501 gaacaactgt ataaagacta taataaaaat aatgatgatg tagtaatttt aggagtagct
     1561 tcacctaatt taggtagaga aggttcaagg gaacatgtag ttaacttctt aaaagaccaa
     1621 ggttatacat tccctgttgt acttgatgaa gatggagcat tagcatatca atatggaatt
     1681 aatgcttttc caactacatt tataattgac aaggaaggat atgtaactca gtatattcca
     1741 ggagctatgg ataaggcaac tatggcatca tttattgaaa atcagagaaa taaataaaag
     1801 tatttaagta taagtttata aatcaattta aggttacaaa aaagtgccta attgtaccac
     1861 ctaaataatc ttgttatagt aaaagtataa acaatacaag aaagggttga attttatatg
     1921 aaaaaaacat ttgatgctat aataatagga tttggaaaag gtggaaaaac attagcaggg
     1981 gatttggcaa acaggggatt aaaagtagct ttaatagaaa agtcaaacaa gatgtatggt
     2041 ggaacatgtg taaatgtggc atgtatacca acaaaaagct tagaaaattc agcaaacagc
     2101 gtaaaaacaa agaatataaa ttcttgggat gaagtacaag ctgaatatga gaaagctata
     2161 gataaaaaag aaactttaat tacaaaactc agagaagcta actataataa attaaattca
     2221 aatgagaatg taactatatt cacaggaatg ggaactttca ttgatgaaaa aacagttcaa
     2281 gtcaaaactg aaaatgaaat ttatgagtta gtagcagata atatatttat taatactggt
     2341 tcaagaccat ttatacctaa tattaaggga attgaaaata agaatatagt atatgatagt
     2401 gagtctctta tgaatctaag aactttacct aaaaaaatga caattatagg agctggattt
     2461 ataggtttag aatttgctgg tatttatagt tcatttggag cagaagttac aatattaaac
     2521 tctaataatg gcattttacc aaatgaagat gttgaggatt cagaagaaat aataaaatta
     2581 cttgcaaaga gaaatgtaaa aattgttaat aatgcaaata taaaagagat taaagaagtt
     2641 tctgaattag caatagtaga atatgaagta gatggtaaat ctaaagaatt aacaagtaat
     2701 atgatacttg tagctacagg aagaaaagca aatactgagg agctagggct agaaaatgca
     2761 ggtatagaat taaatgaaag aggatttatt aaagtctcag aaactcttaa gactaataaa
     2821 gagcatatat gggctatagg agatataaat ggaggtccac aatttactta tatctcattg
     2881 gatgattatc gcatagttat taatcagttg tttggagata agactagaac aactaatgat
     2941 agaaagaata tcccaaattc tatatttata agtccagcat tttcaagagt tggtcttaat
     3001 gttaaacaag ctaaagaaaa gggatatgaa gtattagtgg caaagatgcc agtagaagca
     3061 attccaagag ctaaacagat tggcaaggca gatgggttta taaaaatagt gatagataaa
     3121 aaatccaata agatacttgg agctagtatg atatgtgaaa attcaagtga gattatccat
     3181 cttattcaat tagctgtaga tttagaagta gagtatacat atttaagaga ccgtgtttat
     3241 gcacatccaa ctatgacaga agctcttaat gatattctat ctcctaatat gataaaagaa
     3301 gtataacata agtttaatat ataaacctaa aattatcatt ataagtagat tttagaaaga
     3361 ggctatctca aaaaatagag ataagtctct tttttactgt ttaaatgata aaaatatatt
     3421 tgattttata aaagaagatt atcttatgaa ctaaaaagtt tattttaaaa cactacctat
     3481 ttatgttata aacttataaa tataatatag aatctattat tctgtattat taagtggggt
     3541 taagcgttat aattaattat aaatattgaa taatctaaat ggataagata atggtattaa
     3601 aaattatctt aaaaggtgaa ataatctgta tttaatgtag tacagttata tctttaaagc
     3661 aaggtggtga aatgatataa ataagatatc taaaagaggg tttgatgata gtgataaaag
     3721 atggttttca agtaaagaat taacaagact tataaagtct aaagaagaaa tagaatggtt
     3781 attaaatagg gattacaaaa tagacccagt cgttactttt gttggtgata gatatcagtt
     3841 ttctataaga caaagagatg cattaaaaag ggcagtctgt actgatgaaa aaaatattat
     3901 aagacaatca aaaaggcttt ctctagataa aatcaaagaa gataccgtaa atatagatgg
     3961 ctttaatttg ataatatcca tagaggtagc attatctggt ggtactctag taataggaag
     4021 tgatggcaat ataagagatt tagcaggtct taggggaacc tataagatta tagataaaac
     4081 agaagaagct ttgaatctga taggtaaatt ttttaataaa tacaatgcac agaatcttaa
     4141 attttattta gatgctccag tttctaattc aggaaattta aagtatagga tattagagca
     4201 tgccaaaact tggggaattg aaactgaagt agaattagta aaaaatgcag atgttgtatt
     4261 ggaaaaatta gatagagtag ttagtagtga tgcagtcata gttgacaagt gtataagtta
     4321 ttttaatgtg gctagaggta taattgaaga gtatattaaa gaatgtaata tcataaattt
     4381 aaataaatag ataaatatag gtgagaatat ataagtatat gtagcaatat tcttagtaat
     4441 acaagtaaag tttgaaaata ggagaacgaa tacatatcga atttcataaa atgttgtaaa
     4501 aagacatatg agaggatgga tatgtgtgaa attaactaaa ctaaatatta aaaaatatag
     4561 agcacctata tataaatatg ctttagcaaa tgtaaatttg aggtctgcta aatctactaa
     4621 ttcaagtatc ataacagtaa tacctcaggg agccaaaatg gaagtattgg atgaagaaga
     4681 tgactggatt aaggtgatgt acaattctca agaaggatat gtgtataagg atttggtatc
     4741 agtaagcgaa tatgcttgga gtaacttaaa cttaagagaa gataaatcta caacttccaa
     4801 tataattaca gtaattcctg aaaaatctag ggttgaagtc cttcaagtag atggagattg
     4861 gagtaaggtt gtatatgatg ataaaatagg atatgttttt aattattttt tatcaattga
     4921 tgggaataaa ccaaatgaat tagattataa gtatttttat actgacatga ttaaatttgt
     4981 taacgaaaat aacattaaaa gtactagcga ttatctaatt gtaacagacc ttagaaataa
     5041 atatacttat atttttaaga aagataatgg aggatgggga caactttaca agtggcaatg
     5101 tactataggc aagcctgaga ctccaactat aacaggtata ttttatataa gtggaagaaa
     5161 accttctttt ggaacggatg agtattcagt taagtatgct actagaatta aaggtggata
     5221 ttactatcat tctgtactat atgattcaac aggaagttat ataatagatg gcagacttgg
     5281 tgaagctctt agtcatggat gtataagatt aagtacggaa aatgcaaagt ggatatatga
     5341 taatatacca gatacaacaa cagttataat tcattaagaa aaattttgag ttttttattg
     5401 ggataataat tcttaaatta atagaattat atattaactt ctcataaaga gtactattta
     5461 aaaagtgtaa taagagagtg ctattaacaa aacaaagata ttttgaggca ctctcttata
     5521 aatatacata attaagatat tttcataaac aattctataa tcttatcttt atcaaagaaa
     5581 acaggattag caccagcaca agcatctttc atagagtttt ctgcaagcaa ttcaaaatta
     5641 ggattatcaa cacctacatc ttttaaagat tttgggattc caacctcttc agaaagagct
     5701 tttatcttat ctattacaaa gtcaacacac tctttatctg atttattatt tacatccatt
     5761 ccaataactt cagctattgg tcggaatttg gcaggggctg atttagcatt ttcctcctca
     5821 actattggaa gtagcatggc attacatact ccatgaggta aatcatataa acctcctaat
     5881 tgatgagcca tagcatgtac atttccaaga cctgcattac taaaggcaat tccatttaaa
     5941 aaacaagcat aacacatttg ttctctagct tctatgtcat taccattttt aacagctctt
     6001 ggaaggtatt taaatatctg ctttatagca tataaggcag ttgaatctgt aacatcataa
     6061 gcaccattag ctactacagc ttctatagca tgagttagtg catccatacc agttgctgct
     6121 gttaaattag atggtttgct aatcattaat tctgggtcat ttactgtcat tgtggctagt
     6181 gaattggtat caaccattat catctttaca tgtctgtctt catcagttat aacatagttt
     6241 attgtaactt cagcagatgt accagcagta gtagttatag ctatgatagg aagacatttt
     6301 ttagaagttt tgttgatacc ttcatagtct ctaatatctc ctccattagt ggctaatata
     6361 gagatagctt taccacagtc ttgtggagaa ccacctccta tagttataac gaagtcgcaa
     6421 ttttctgatt ttaatatttt aagtccttta ttcacattag ttacagttgg attttgttta
     6481 acttcatcat aaacaacaaa gtctacatct atctgagata aaatatctgt tacttttttt
     6541 acagtaccat ttgaaactag aaatttatca ctaacaataa atgctttttt agttcctaat
     6601 gatttaattg aatctttagc ttcattaaga catcctttcc caagtaaatt tattggtggt
     6661 acataataaa tgctcataac taaaatctcc ttttattttc attgattttt atgtaaaaat
     6721 atatatctaa caattttcac taacttactc ataatatatc gtatcttaga ttgagatagg
     6781 attgttggtt cttatataag aacttaattc gtaattacga ttatgattag aatttaacat
     6841 agtattttta tcatgtcaat aaaaatataa aaaatattat aaaaaaaaca aagttactgt
     6901 agttaaatca gcaactttgt ttttatagta taaatcaaat aaatacatta gtttaaaaca
     6961 aaatttttaa ttattatgtt ttgatatctt ccatatacag aatattaatc taatttatta
     7021 caatgtcttc attacgttct tccatcattg attgaatatt tttctgagca tttaagagta
     7081 atttttttat cagttctatt ttttcgtcat tagctctaaa taaaggcagt gttacactaa
     7141 cagcagctct tatacatcca ttttttctta ttggtacggc aagacatttt aggtgttcca
     7201 ttgattcctc acactctgaa gctatttgtg ttctttgaac ttcaagaagc tggtcgtata
     7261 aaacatcaaa atctgtaatt gatttttctg taaaaccttt tagaccatct ggatataagc
     7321 ttattagttt ttccttgtca aaatcacata aaagagcttt tccaagacct gtacaattag
     7381 caggtatttt ttttccaaca taagaaataa gtctaattgg ttctggagaa tctacttttc
     7441 caatataaag aacttcatta ttgtcaagta taccaagctg acaagtttca gaacaggaat
     7501 taactatatt agtcatttcg tcaattatat agttaaaaat agttcttttt tcaaaaaatg
     7561 aagaccctaa tgtgtaagaa cgtatcccta tagaatattt ggatgtgttt ttatcaatag
     7621 taatgtattt tctagcatta agtgtatgaa ctataggaaa gatgctactt tttggagcat
     7681 tgataacatc tgctatttct gtaagagtat atccattttc ggattctgct aatgcttcta
     7741 aaatatctaa tatcctagct gttgggcgat gattatctga agtcataaat ttccccctct
     7801 ttttattaat ttaaagtatt agaatatgtt gattttagca cactatacga tattttacaa
     7861 tgtagaaaat aaatatgtaa atttatatat aaataagctc ttgatttaga tacagataga
     7921 tattttttta gactgtacat taaggaataa ctaatttcca aaatgtacct aaagaaagta
     7981 agtgatattt agttatataa tttaagtaaa tatatgcaat agttattttt atatgtaaat
     8041 atcaaaatat tatgaactta tattttatct attttttaca ctgtataaaa actttatagt
     8101 tttgaatact atataatttt aaatacgctg tatttttgca tataaggaag tcatttgata
     8161 atatacaagg tattatcaga aagtttgaaa aaattttgac aaaaatttat tttttttatt
     8221 gactcataaa taggaaaatg ctatcattaa ttcaaagatt cgtaattaaa aacttagttc
     8281 ttaaatacga accgaaacgg ccgttagaat taaagctaaa aacttaataa gagtaattaa
     8341 atatcatgtt ttaggaggat taactttatg ataatggatt ttattaaaaa aattcctgca
     8401 ggtatgatga tagtgccaat gtttatagca gctttcatta ataccttttg cccagatatt
     8461 gtacaaatag gttcgtttac gacagcagta ttttcaagtg ttgggtctgc aactataatt
     8521 ggggttcaac tggtatgctt gggaactcag cttcaattta gagaaatgcc aaaagtttta
     8581 aagagaggtg gtatacttct tggttctaaa tttgctatag gtgctttgat aggtatactt
     8641 ataggaaagc tatttggaat ggatggtgtc cttggtctta caacattggc tgttataagt
     8701 gcagttacaa atagtaatgg tagtatatat ctttcattga tgacttcata tggagatgaa
     8761 acagattgtg caactatgag tttacttact ttaaatgatg gaccattttt tacattaata
     8821 gctcttggga cttcaggtct tgcaaatgta ccattgttgt cattattggc tgcaatagtg
     8881 ccaatacttg ttggtatgat aataggaaat tttgacaaaa aaatgaaaga gtttttagag
     8941 ccagggtgta atttactcgt accttttgta gggtttgcac ttggtagtag cataaatctt
     9001 gcaagtatac taaaaggagg tttatcagga gtattacttg gattaatatc tgtatttgta
     9061 ggaggtatat ttatagtatt ctgtgataaa tttataggaa aacgtccagg atatgcagga
     9121 tgggctgtag ctacaactgc tggaaatgca attgctgtac ctgctgctgt tgcattagtt
     9181 gacccatcat ggcaaccata tgttgcaaca gctacaacac aagtggcagc cgcagtagtt
     9241 gtcactgcaa tattagtacc tcttataact agttggtggg ctaagaagta tggatgtcct
     9301 aagttcccaa aacaaaatga ggatggtaca ataacagctt aatttatatt acagtgagtt
     9361 gttggtaaaa taataaattc tttaattaga ttttaggata ttaataaaat tatattattt
     9421 ataaaggaga gcaagaataa tgataaatgc agtgataatt gatgaaaaag ataatgttgc
     9481 agtagctatt gaagcaatag ctaaaggtag tgaaattagt tttaaattaa aagacaaaac
     9541 agtaaaaacg attacagcac tagatgatat aactatttat cataaattgg ctataaaaga
     9601 tatggcccaa ggtgataaag ttactaaata tggtgaacat ataggagaag caaatcagga
     9661 gataaaaagt ggtcaacatg tacatattca taatgttaga agtgttagag aggatttgga
     9721 taaacaagtt atgactaata tataaaataa tatttataga gttataaaag ttgtattcat
     9781 tgattttagt gtcaaatagt ttttaaatag gtttttagct gtttgattag tgagtgtaat
     9841 aaaaattata atatataaaa attatagaaa gtatatagac tattttagga ggaaatcagt
     9901 atgaaatttt atggatataa aagaccagat ggaagagttg gtataagaaa ccacatatta
     9961 atattgccta cgagtgtgtg tgcatcagat acaactagaa taatagcatc acaagttcaa
    10021 ggggcagtta cctttaataa tcagaatgga tgttcacaag tacattcaga ccaacaatta
    10081 actatggatg ttatggctgg ttatgcagca aatccaaatg tatatggtgt aatagttgta
    10141 tctttgggat gcgaaaattg ccaaaatgat ttggtagttg atgcaataaa agagagaaca
    10201 aataagccaa taaaaactct tgtaatacaa gaagaacatg gaactttaaa aactatagaa
    10261 aaagctgttc gttatgctag agaaatggca caagaagctt ctttgcttag aaaagaagaa
    10321 tttccaatat ctgaacttat actaggaact gaatgtggtg ggtctgaccc aacaagtgga
    10381 ttagcagcaa atccattaat aggtgaatta agtgataaac ttgtcgattt aggagcaaca
    10441 tcaattttaa gtgaaactac tgaatttata ggtgcagagc atatactagc taggagagca
    10501 gtaaatgaag aagtaaaaga aaaaatatta catatagtac atagatatga aaattcatta
    10561 aaattggttg gagaagaagt tagagaaggt aatccatctc ctggaaatat agctggtggt
    10621 ttaacaagtt tagaagaaaa atcacttggt tgtatacata aaggtggtca tagacaaata
    10681 agtgaagtat atgattatgc caaacaaatt gataagaaag gtcttgttat aatggatacc
    10741 cctggaaatg atgcttcatc tgttgctggt atggtagcag gaggagcaca aatagtggta
    10801 tttagtacag gaagaggaac accatcagga aatccaatag caccagtaat aaaaataact
    10861 ggaaataaaa taacttttgc aaatatggaa gataatctgg attttgatgc tagtccagtt
    10921 atatatggtc cacaaacaat ggaagaatta acagatgatt tgttaaatat ggttgtagat
    10981 gtagcaaatg gaaaacaatc aaaagcagag tcccttggat atactgaaat ggcaatagct
    11041 agagtatgca actatgtata atctttaagt aagaaaataa atacatagaa ttttaataaa
    11101 gggtgagatt atgaagtttg taagttttac tgaaggagaa aatgaatgta aaagaactgg
    11161 agttttttct aaagatgaat tatttattat agatgttaac tctttaaatc tcagtagaaa
    11221 ttttaaggat ttaaatgaat taattcaaaa tgtatctagt gaagatatta ttaagttgaa
    11281 aactattata aatgaaagta cattctataa ttatgatgtt tttaaaagag agagatgtac
    11341 tgtacatgtt cctatagaaa aaccaataca tgatatctta tgtgtgggag tgaactataa
    11401 agaccatctt gaagaaactc aaactcactt tgatgatagt tttgaagaac cacaaaaaac
    11461 ggtttatttt acaaaacgtg tgacaaaggc aataggtcca gaagatgaga tagatggtca
    11521 ttttgagata aataatcaac tagactatga agttgaactt ggagtagtaa taggtaaaac
    11581 aggtgttaat attagaaaag aagaggtaga agattatatc tttggttaca ctgttataaa
    11641 tgatatttct gctagaaagt tgcaaaaaga acatgttcaa tggtttagag gaaaaggatt
    11701 agatacattt acctctttag gtccatgtat tttacataaa agtgcagttc cttttcccat
    11761 aaaacttaaa gtatgtagta gggtaaatgg tgaagaaaga caaagttcta atacagggtt
    11821 attcatatca gacgtttcag acatagtttc agaactttca aaaggtatga ctttagaggc
    11881 tggagacatt atagctactg gaacaccttc tggagtaggg atgggatttt cacctcctcg
    11941 atatatgagt agtggagatg tagttgaatg tgagattgag agtattggaa ttttaaaaaa
    12001 ttacattaag taactgtttt aatataatta atattaaagc taaatctgat gtattaatga
    12061 acatattaaa ataaatttta ggagtttaaa ttatgaaagc agaatttttg acaccagttg
    12121 ttacaatatt tgatgaagaa ggaaatttag ataaagaagg taataaaaaa gtgtatgacc
    12181 atcttataga tggaggtgta gatggactag tcataatggg aagtactgga gagttcttta
    12241 atatgtctat ggatatgcaa aaagaactta tagatttagt aactagtcat gtaaataaga
    12301 gagtaaaagt gtttataggt acaagtcgta tgtgtataaa tgaatctata gaactttcaa
    12361 actatgcaca taatagtgga gcagatggtg ttatgataat aagcccttat tatttctctc
    12421 ttgatgatga tagtattgag ttattttata gtgaaatagc taagaataca gaggctgaaa
    12481 tatatttgta caattatcca gatagaactg gttatgattt aagtccagaa cttacattaa
    12541 gacttgcaag aaaacataaa aacatagttg gatataagga tacagtaact ttgatgggac
    12601 ataccagaga tttaataaat actatgaaaa tagagttccc agaatttaag gtttatagtg
    12661 gttatgatga aaactttata cataatctaa tgtctggggg agcaggttgt ataggggggt
    12721 tatcaaattt aattccagaa gtttgctcca aatgggctga agcttacaat aatagagata
    12781 tggagaaggt tatagaaatt caaaaatata ttaataaagc aatggatttt tacttaataa
    12841 ccaatccatt tataccagca atgaaaaaag ctatgaatat cagagggtta caaataagtg
    12901 attatatgac tgctccattt gtaaaaccaa atgaaaaaca agtatgtgaa attaaaaaat
    12961 tgatgaatga attaaatata tgttaaatca accttgcaaa gaggtcaaca ataaattaca
    13021 attatcttaa taaaaattaa attaaaataa tactagggta ctatattagt ttaataggga
    13081 aagtgatgaa aattcactac agcccccgct actgtatacg gatacgaaat caaaatccac
    13141 tgaaatttat aaaaattttg ggaagggtga tggaggaaaa gccgtgagtc aggagacctg
    13201 cccagtatta tataagttca atttagggtg gagattgtaa tttataataa aatgaagtat
    13261 caattcgttt ttgtaaagtt gatatatgaa cttctcctta ttaaattagg agaagttttt
    13321 tattttatta aaaggttata cttattaaag atgactttag gataacttta ataagtatac
    13381 tttaaaaagc atatattatt aaagtttttt agccactcat aaaaaataag atattggagt
    13441 gaaagagatg aaatttaaat caaatgtttt aaagcagttt tttttaattg ctatggtaat
    13501 ttttatggta ggaggtctta tagggtgtag taaaagtgac aaagagaata aacaagctag
    13561 tactggtaag aaattagaat taaaatatgc tagtggattt agtgttgaag atttagaaga
    13621 tggaattaaa aaagtaactg atggagataa tcgagaactg attttaatcc caaagaagat
    13681 taaaattcct gaaaaatata aaaatgctaa tatagttaga acgcctgtgg acaatgtatt
    13741 aatagcatct acaacacagg cttgttctct aagaacaata gatgaactag atagtataaa
    13801 agccgtaaca acacaagagc agtactggac tattaaagaa attaaagatt taatgaaaga
    13861 tggaaaaata cattacgttg gttcaaattt agcaccagat tatgaaaaaa tagtagactt
    13921 aaatcctgac ttaactatag tgagtagtgg tttatcagag gctgatacaa aatttattga
    13981 caaattaaaa gaattagggc ttaattatgt tgtagacaat gagtataaag agcaaaatcc
    14041 atttggaaga atggagtgga caagtttaat agctgcattt tatgataaag aagatatggc
    14101 tactagtgaa ctaaataaaa ctgtacaaaa ggtagaagaa gtaagtaaaa aaataaaaaa
    14161 caaagctaaa cctaaagttg tatgggcatc tgtatatgaa ggaagtgctt atatagctcc
    14221 taaagattca tatgtggata atatgataaa aatggctggt ggaataaatg catgtgcttc
    14281 aatagattca aatgaagggc gagtaagtat tgaacaattg catgaagttg caaaagatgc
    14341 agatatattt gtatactcat caagttcaga ttatgcacca aatttagatt ctataaaatc
    14401 tagtgcacct gtattggctg atttggatgt tgtaaaaaac gggaacctat gggtgtttgc
    14461 tccagattat tatcaatcag tagataagac agacgaatta atagtagatt tagttgagat
    14521 gttccaccca ggtactacag gtgaaaaaat caaacattat ataaattatg ataaatagta
    14581 cagagaggac acaaaaagat aggttttata gattttagtt aatatagctt tgctaaaagg
    14641 agtatacaaa tggattttat caataaaatg agaataagga atacgacaat atttatagta
    14701 ttaattataa gtatgatact tactttttta gtgtgtgtag catttggctc tgtaaaaatt
    14761 gaatttaaag aagtaataga tgttgtatta agaggtatgg ataatacaga acattataaa
    14821 ataatataca atataagaat ccctagagta cttgcaacca tggtaggtgg agcatgccta
    14881 gcagtatcag gtattttatt acagatattt tttaaaaacc caatagttga accgtatata
    14941 ttaggagtat cttcaggttc tactttatca gtagctctca tgattttggg aggatacaca
    15001 ttgggatttg agagtgtaaa ttcaatgagt atatttgtag cagcttttat aggagcttta
    15061 attgtaatgt ctatagtcat tttatttgca aataaggtaa agaatataac aactctttta
    15121 ataataggaa ttatgaccgg atatgtatgt agtggtataa caaatgtgtt acaatcattt
    15181 gcaaatagtg ataaattaaa agaatttact atgtggtcta tgggaagttt ttctggattt
    15241 acatgggaaa aggtagaaat acttataata gttggtatat taggtctttt gatgactgta
    15301 ttcataataa aacctttaaa tgctttttta ctaggagaag agtatgcaaa aagtattggc
    15361 ataaatataa gattttttag agttttgata gtatttatat ctagtatatt agcagcaata
    15421 gtatcagcat ttgcaggacc tatagggttt attggacttg cagtgccaca aatttcaaaa
    15481 atgatattta aaacatcaga taatagagtt ttgataccag tagtaatatt tttaggcagt
    15541 atagttacag ctctttgtga tttaatatca agagtaattt tatcaccagt agaattacca
    15601 ataagtacaa taacatcttt attaggagct ccaattgtag taatgttatt ggtgaaaagg
    15661 aaggataggg cttagacatg ttgaaaacta ataatttaag tgtaggatac aataacaaag
    15721 ttgttataag taatataaat gtagaagtaa aaaatggaga aatactatgt cttttgggaa
    15781 gtaatggagc tggaaaaact accctattaa ggtccttatc taaacttata tcaccaataa
    15841 aaggtgaaat atatttgaat ggtgtaaaca taaattgtat atcaagaaaa gcattatcaa
    15901 aaaaaatggc tttagtactt acaaatagat tattaggtga tttaatgact gtacaagata
    15961 tagtcaatat aggaaggtat ccatatacag gattttttgg gagtctatct aagaaagatt
    16021 taataatggt agatgaagca ttggagtctg tagatgcctt acatttaaag aaaagatatt
    16081 ttgatgaact aagtgatgga gaaaaacaaa aggtgttggt tgcaagggct cttgttcaag
    16141 aaccagaaat aattatatta gatgagccta caactcactt ggatataaaa catagattgg
    16201 aactcataaa catactgaaa aaattaagta aagaaaagtc aatatcagta atactttcat
    16261 tgcatgaaat agacatagca ttaaaaagtt gtgataaagt tgctcttata aaaaataata
    16321 aagtcatagc ttatggccag cctgaagatg tagtagatga ggatattata aattcgcttt
    16381 atgaattgga tgataaaaac tttaatagtc ttttaggttc agtagaaatt agtaataaat
    16441 ctaaaaatga agtttttata attggtggtg ggggtaaagc aacaccaatt tatagagctt
    16501 ttactaaaaa aggtataggt ctgtattctg gaataataca tgaaaatgat attgattatg
    16561 aaattggtag aactatgggg ataaagatgt ttacagaaaa tccatttgag cctataagtg
    16621 atgagagttt cgatttggct attagaaatt taaatgattc caagattata atagatacag
    16681 gatttagtgt aggagagaca aataagagga atatagatat tataaaagaa gctttaaaat
    16741 tggataaaaa agtttattct tttagaaata gagatgagag taagaagtat tatgacagtt
    16801 tggatagtaa gatagaacat atagataaag tttctcaaat aatcaatagt gcagatataa
    16861 ataatttatt ataatttatg aaataaaatg tacttatgtg ttatagaaaa aagagtatag
    16921 attataattt ttttgaaatt acaatctata ctcttttgtt attttgttta tgtatttata
    16981 tatttataaa actttagcat tttatagata accaatagct tcctctctag ttaaaaaaaa
    17041 gtgaatccct gtagtcgatt ctttccatct attttcatta aaatcttcag ctacaatcgt
    17101 ttctcctact ctatatataa aattttcatc agcatatgca cgagcacttt taaaagattc
    17161 tttaccatct acactagtaa ttgacagtac ttttgcctta tcacatctac aagcatttga
    17221 tgtagcagac actctttttg cgtcgctggg tattaataat tgtaccattc taaaattaaa
    17281 acatttttta tagcctataa atgctcctgt ttcaggacaa tacaatttaa aaaaacgagt
    17341 actttcatct gatattactc catttaaagt agcatattct aaattggaat tttctaaatt
    17401 agctcctcta atatctgagt tagataagtc acaaaatcta aaacttgcac cacttaagtc
    17461 tgcatttttt aatatagaat tttttaatga acaatccgaa agataagaat tattaaaaat
    17521 agagttttct aaatctgctt tttctaaatt taaattgata aggtcagacc acgaaaagtc
    17581 aatattactt aagcagtatc ctcttaagtc catattttct aatcttaatt cttttaaatc
    17641 tgccttttct ccagcgttat tgtttttaag ccataaataa tgtttctcaa gtgttttttc
    17701 taattcttgt ttagaaatag ttttcattaa aatcaccttc ataaatttat ttttatatat
    17761 gtagtagcaa aatagtca
//
LOCUS       DAMJYR010000076        17757 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.076, whole genome shotgun sequence.
ACCESSION   DAMJYR010000076 DAMJYR010000000
VERSION     DAMJYR010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 17757)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17757)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17757
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.076"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(<1..464)
                     /locus_tag="PC471_002457"
     CDS             complement(<1..464)
                     /locus_tag="PC471_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HDF2789581.1"
                     /translation="MKICITVGHSILKSGACTSADGVINEYQYNKSLAPVLADTFRKE
                     GHKVDVIICPEKQFKTKNEEKSYKIPRVNSGGYDLLIELHLNASNGQGKGSEVLYYSN
                     KGLEYATRICDKLGTVFKNRGAKLDKRLYILNSSKPTAVLIESFFCDNKEDY"
     gene            complement(464..895)
                     /locus_tag="PC471_002458"
     CDS             complement(464..895)
                     /locus_tag="PC471_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HDF2789582.1"
                     /translation="MNVTIVFLATNIFIKLVILAIAFDTLLGCLRAIKTHQFNSSFGI
                     NGGIRKVAMIACIFFLAVVDILTKFNFLFMLPQNWVDFLRLNHLGISEFFSILFILYE
                     SVSILKNMYLCGLPVPKKLKDRIGNLLDTMTDELNIKGGSK"
     gene            complement(957..1139)
                     /locus_tag="PC471_002459"
     CDS             complement(957..1139)
                     /locus_tag="PC471_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015981517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2789583.1"
                     /translation="MNINNIVVRILAERILSGGLNPLKNREFELDDVTNTEYRKAVED
                     YIIEHSGVVEGTEPTK"
     gene            complement(1139..1432)
                     /locus_tag="PC471_002460"
     CDS             complement(1139..1432)
                     /locus_tag="PC471_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2789584.1"
                     /translation="MNRANRIIYDQTGKILLQTGEATGDILEHDIITQLHCIDVPYGS
                     IDYEKYRIIGINIETKEPISEEIPVYVNEEEKRIQELENQLLIAENEKVGGIL"
     gene            complement(1453..2136)
                     /locus_tag="PC471_002461"
     CDS             complement(1453..2136)
                     /locus_tag="PC471_002461"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2789585.1"
                     /translation="MTTKLTDNASLGELMTALQSVQTDFQTGKNNLSSALGNPFVGTD
                     KLETTKTKIETLKNSLAVNLTNKNVSANETESIQSLINKVGNIKISNYAAGTTSVVKN
                     TSLYAKTFYLNSTSNPSYWISITNLNFLPNVFIAECEYTTSDKWNKYYVIATSYFPSS
                     TVILTDFVLNVSFNCEFSASTLNSGASKLYENNKRDVYITGGSIRVPAFTESSSTLSS
                     YKWYALKIQ"
     gene            complement(2150..2956)
                     /locus_tag="PC471_002462"
     CDS             complement(2150..2956)
                     /locus_tag="PC471_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDF2789586.1"
                     /translation="MATDKSYYTILTDIGKAKIANASLVGEKVDFAKIQLGDGGGNEY
                     NPTEEQTALKNVVWEGNVGNVKTDEAMANCLILESLIPANAGGFIVREIGYLDTEGNL
                     LAISKYRSAYKPKVEDGAVIDMKVKTIFVVSNVNNIELKIDPTIIFATLKDLQDLDTK
                     IDTTKTELTSNIEIAKTELNNKIGDTTLLETTDKTSLVGALNEVKTSVDSIETTAEKT
                     SIKDTDNLFESDNVEGALKELATKQNLLDTEVNGQREKGINIANNLIDMI"
     gene            complement(2972..3589)
                     /locus_tag="PC471_002463"
     CDS             complement(2972..3589)
                     /locus_tag="PC471_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmfQ family protein"
                     /protein_id="HDF2789587.1"
                     /translation="MDKEINLINYLPQILQDKEEYIKVFNVENKEIKTLHDKLKDLSN
                     DQFLEDLTISGIKRWEKIMSITPKSNESLEDRRFRIFSKYISKLPYSERFLRNWLDNV
                     VGEGNYELTINNATYNIHLESDARNQDWFNEVHTFVSSIKPCNMSLDYTRVLVSKDNG
                     MYFGATTVTGHEITIYPWSPSDIETQGEIKIMSGNGFGYQEITIY"
     gene            complement(3582..4631)
                     /locus_tag="PC471_002464"
     CDS             complement(3582..4631)
                     /locus_tag="PC471_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HDF2789588.1"
                     /translation="MFELMTFENIIKRMLDSVPDTFDKREGSIIYNALAPVAIELTET
                     YIAMDELLDQTFVDTASYYYLEKRCKERGITPLPATNTIAKGVFNIDIPLDSRFNLGE
                     YNYVAIERISEGIYKMKCETAGPIFELGQLIPIEYIDGLETAELTEILINGEDEESED
                     SLRQRYYDSLNSQSFGGNIQNYKDEVNKIQDIGGVKVYPVWNGGGTVKLVIINSNFKV
                     PSEDLVNLVQEEIDPIGHQGQGLGLAPIGHRVTVEGVTSTTINISAEITYKSGYTWEN
                     IKSIAEEAIDDYLNELNMSWEDEENLIVRISQIETRLLSIDGVLDITNTMINDVKSNL
                     TIDSNSIVVRGEVVG"
     gene            complement(4632..5051)
                     /locus_tag="PC471_002465"
     CDS             complement(4632..5051)
                     /locus_tag="PC471_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2634 domain-containing protein"
                     /protein_id="HDF2789589.1"
                     /translation="MLPTDNIDYDIEDVSIINFDVRQEPSKTFKLHIEKNRVDGICDD
                     VEALKQTIFLILNTERYEHLIYSRNYGVELNDLIGEPISFVIPELERRIKEALIQDDR
                     VENVDNFEFQNIKGKVHCKFMVHTKYGNIKAEKVVSV"
     gene            complement(5056..5316)
                     /locus_tag="PC471_002466"
     CDS             complement(5056..5316)
                     /locus_tag="PC471_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2577 domain-containing protein"
                     /protein_id="HDF2789590.1"
                     /translation="MSQELLQIIKKAAMDAVETSNPMQIAFGTIESVNPLVVKIEQKA
                     SFEEFFLIQTDTFKRYTDKKIGDKLVLIRMQGGQQYLILDRM"
     gene            complement(5330..7297)
                     /locus_tag="PC471_002467"
     CDS             complement(5330..7297)
                     /locus_tag="PC471_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HDF2789591.1"
                     /translation="MSDDLVLANDRDVRLVIAHWEDFYEPVVLDGITWEIERRGAPSK
                     LEFTIVMDDILEFCEGNSVRLYYKGIGIFYGYIFQKKRDKENHIKIVAYDQLRYFKNK
                     DTYVYNNKTASELVKMLTKDFNLKYNVIEDTKYKISRVEENKTLFDMILTALDDTLRE
                     KKEMYVLYDDFGRITLKNVASMKLDTVMNNDVIEDFDYNSSIDSDTYTKIKLVRDNEE
                     SGKRDVYIAQDSAHMRSWGILQMFETVDKNMSEAEIKQKCDILLKLYNKKTKSLSLKN
                     VLGDIRVRAGCLIPVFLDLGDIELQNYMLVEKVKHTFENNSHFMDLTLVDGDEFASYS
                     SSSYSSGNANNKDEKKNGPAQNTTSKEDNDMINKLNKVFKGKLSNTGNIFVKYSNAYK
                     VNPALMAAISMHESARGTSNIANTKNNFFGMKGMSFGSVDEGIKRGISNLSRNYIHIG
                     RKTLESIRDKYAPLYDSPLNKDWVPGVGKFYKQITGNAYSSNSAGTGVGSNEEAEKNL
                     KDTTYQVQNSNNANTSTNNNSKVSKVIQEAKNQLGKPYKWGGNGPKSFDCSGLMVWAF
                     KRGAGINLKRVSADQSKDSRGKLLCNINDVKAGDLVFFKNEQGKVHHVGLYIGNDQYI
                     HAPQTGDVVKISSLSGRQKKKHDFARARRFF"
     gene            complement(7290..7985)
                     /locus_tag="PC471_002468"
     CDS             complement(7290..7985)
                     /locus_tag="PC471_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HDF2789592.1"
                     /translation="MAYDFYLDGVQLPIAPGKLEIKVTNKNKTVDLINTGEVNILKKE
                     GLSEISFEAEFTHNKLPFYKGAFKDVQFFLSKLELLKTDCKPFQFIVSRQLGNKVLFN
                     TNIKVSLEEYNIVEDAENGSDTKVAIKLKQYRDYSTKKLVPATPKTTSDAGRPNVKIE
                     KKRVDSVNEPNTKTYTVKAEDSLWAICQKQLGNGSLYKKVYELNKSMMDKANKGKNLS
                     KYTIYKGQVLRLV"
     gene            complement(7997..10420)
                     /locus_tag="PC471_002469"
     CDS             complement(7997..10420)
                     /locus_tag="PC471_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HDF2789593.1"
                     /translation="MTTSINTTINSLERLQRELRNPLDEGGIQASVKIFDRMAPALKN
                     MNTSINATAKSLERLQQKLNNPINAGNIQASQQNLKNIESILTRIEQKIGRNTNEQEN
                     FNNKIRKGSEEGSLLVSKLKSLAGVYIGIRGIESITKAADTIASTKARLNLMNDGLQT
                     TEQLNKMIYLSAQSARASYADTAAQVAKLGILAGDAFGSSAEVVKFTELMNKAFVIGG
                     TSASEASAAMYQLTQAMGAGKLQGDEFRSIMENAPLLATKIADAMGKTKDQLKELSSS
                     GAITADVIRNALFKASDEIEKKFASMPITFSQALTMMKNDAYMIFGQTLGKISGALQS
                     VRFSEIVVSMRNVMIAISSNIYDTLNIIKNILNSDFFSDFVQGITLGAVLIIQGLGGI
                     TNAALSVVNVFAQNWNIIEPIVYGVVAAIAIYSLALAGMWLWEKLVAASKFAMMFAQM
                     LLNTKTLAGYAYLIKYAAGQWIANEAMLACPIFWIVAGILAFIVVVFVAVAAVNKFAG
                     TNLTALGVIVGAVFAAVAFIQNIMIWLFNRCVDVNEGITNGWNQCVFFMKQAIAKGVI
                     FIIEKMASLNDSVNNAGNALGKAFVTGANIAIRGVNKLIDLLNKIPGINIGKVGEATF
                     TPVKADNSYIKQQINSLNRWVGDAPEKIKLERMQYKDIGAEFQKGNALGTKWQNAISN
                     KLKDTFDINKMLEDAKDKLGLKDLWDKNNPLNNLGGFGGDLGKNVKDTAGNTAKMAKT
                     MDKSQEDLKYLRDIAEQEVINRFTGVNIKIDMNNTNNISKDADVDGIVNILTEKLNDA
                     MVVSAEGVV"
     gene            complement(10549..11310)
                     /locus_tag="PC471_002470"
     CDS             complement(10549..11310)
                     /locus_tag="PC471_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HDF2789594.1"
                     /translation="MGLFRKSKEPCCICGKEKTDQKIKDGSVCIECLMNYMEYKDKQF
                     TFKDGLPTKSEIKSVLDSKKQNDFLVEKFNVTKEINSFIKFDETNKLIYIETKRKNGK
                     IKKNVYSFEDITGFELLEDGETVTKGGIGSALAGGVLFGGTGAIVGSVVGKKKTKKII
                     ENLQIKLTLKAISEPVAYINLISGKTKTNSIGYERAYEDAQEILSVLSIILKDTENNS
                     NNSNADEILKYKNLLDLGAITEEEFNIKKKELLNL"
     repeat_region   11436..11663
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=11501..11531
                     /rpt_unit_seq="atttacataccacatagttaatataaaataa"
     gene            complement(11941..12099)
                     /locus_tag="PC471_002471"
     CDS             complement(11941..12099)
                     /locus_tag="PC471_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2789595.1"
                     /translation="MDNFLQGVLASLVASLIVYLTSKLFKKVKSHSNTTKSDFDFKLT
                     IKFKRNKH"
     gene            complement(12422..12862)
                     /locus_tag="PC471_002472"
     CDS             complement(12422..12862)
                     /locus_tag="PC471_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HDF2789596.1"
                     /translation="MGDLNAFLSQNAIKVENRKYVASERFIGEDGKPIEWELKAIDSD
                     RDRQLRKNSAVRVPVIGKKGKATGQYTSETDFNTYTLKLCVETIVFPDLHDAELQNSY
                     GVMGAEELLTTMLTPGEYTDLSSEVGEVNGFDRTFEDKVEEAKN"
     gene            complement(12864..13121)
                     /locus_tag="PC471_002473"
     CDS             complement(12864..13121)
                     /locus_tag="PC471_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2789597.1"
                     /translation="MVKIKDKYEIKDSISFDYSKKRPIEECVGEMYRKAGLFLVDIAD
                     KLALDTIEGSSLKPITIKIKLDEDGIATIEKETKYLIMEVE"
     gene            complement(13174..13650)
                     /locus_tag="PC471_002474"
     CDS             complement(13174..13650)
                     /locus_tag="PC471_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HDF2789598.1"
                     /translation="MSKNITMNVRDAISGSKAECFVTIKGKRYNFMQAINLEAKMEKN
                     KSEMPILGSITKGNKSTGSKFSGSATFYYNTSIFRELLYEYKETGEDIYFDMQITNED
                     PTSSVGRQTIILEDCNLDSGIIAKFDADGEYLDEDVDFTFENWRIVEKFNIANGME"
     gene            complement(13669..14979)
                     /locus_tag="PC471_002475"
     CDS             complement(13669..14979)
                     /locus_tag="PC471_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath family protein"
                     /protein_id="HDF2789599.1"
                     /translation="MALGGGTFVAQNKVLPGAYINFISAKRATSSLSDRGIVAMPLEL
                     DWGIDEEVFQVTSDDFEKYSTKYFGYDYTHDKLKGLRDLFKNIRLGYFYKLNKGVKAS
                     CSIATAKYSGTRGNDLKVIVTTNIDDNTKFDVVTLLDNKKVDTQIAKVITDLQDNDYI
                     TWKKDATLEASAGLVFTGGTNGEAVTGAEYQAFLDKIESYSFNALGCLATTAEIKSLF
                     VEFTKRMRDKVGAKFQTVLYKKNDADYEGVVSVENKIKDTGLLESSLVYWTTGAIAGC
                     DINKSNTNKKYDGEFDVDVNYTQIQLEEALKTGKFIFHKVGDEVHVLEDINTFVSFTD
                     DKNDDFSSNQSIRVLDQIANDIATLFNTKYLGEVPNDKSGRISFWNDVVKHHEQLQNM
                     RAIEDFKADDVSVEPGSDKKTVVVSDAVKVISAMGKLYMTVSVS"
     gene            complement(14980..15156)
                     /locus_tag="PC471_002476"
     CDS             complement(14980..15156)
                     /locus_tag="PC471_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2789600.1"
                     /translation="MSKTLSKETDYKFTKEQIVNSKKYVNRKDLLNAILRENELYSFS
                     EVEKIINNFMKGVS"
     gene            complement(15149..15586)
                     /locus_tag="PC471_002477"
     CDS             complement(15149..15586)
                     /locus_tag="PC471_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2789601.1"
                     /translation="MINNIIDGISVKLDKSFGNEYTIYSEDVEQGINEPCFFIVPINS
                     SKALYPNGRELKKNSFDVHYLPRSKDKNFEINEIAEMLLEELEYIEIDGDLVRGTNMN
                     FEIVDNVLHFFVDYNYFTIKSNDTDKMDTVELFGGLKRGDNFE"
     gene            complement(15579..16010)
                     /locus_tag="PC471_002478"
     CDS             complement(15579..16010)
                     /locus_tag="PC471_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HDF2789602.1"
                     /translation="MARGGSVDFKELKKLQRKLQRLEDSQLDKFLKDCARELAARLVR
                     KARKRGRTPKKTGTLKDGWGGIAYARKLPVTKVGDNYVIEVINPVPYASFVEYGHRTR
                     NHKGFVKGKYMMTISIMELRGEAEAIIEKKLMVLLKKVFDD"
     gene            complement(16010..16372)
                     /locus_tag="PC471_002479"
     CDS             complement(16010..16372)
                     /locus_tag="PC471_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015984843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2789603.1"
                     /translation="MVNRTRKAIEMMYRDKCTIVEYQPIKDPITKRTNNKEVVVLENQ
                     PCKLSYKNTTSATDGKVAKLTQTIKLFISPNIEIKAGSKLIITNQNNITKEYIRSGEA
                     AIYPNHQEIILELLEDKA"
     gene            complement(16366..16767)
                     /locus_tag="PC471_002480"
     CDS             complement(16366..16767)
                     /locus_tag="PC471_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2789604.1"
                     /translation="MENNLIDDIEKRLESFEYILKDGDKWLIGFVREKIENIIKLDCN
                     IKTMPIELKEIEVDMIVGEFLFTKKNMGQLDIESLNFEAVEKSISEGDTKVDFAIGSG
                     SQTPEQRFDTLVNYLLTYGKNKILTFRCLRW"
     gene            complement(16777..17010)
                     /locus_tag="PC471_002481"
     CDS             complement(16777..17010)
                     /locus_tag="PC471_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2789605.1"
                     /translation="MPQVKKLNRILTIEECRIDDFLEMGYDLIDETGKVVKYGKSLNV
                     KDLIAENNILRSKVESLEEENKQLKEKNKLTKK"
     gene            complement(17029..>17757)
                     /locus_tag="PC471_002482"
     CDS             complement(17029..>17757)
                     /locus_tag="PC471_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsid protein"
                     /protein_id="HDF2789606.1"
                     /translation="VDSNRDTIAVAQRNYDNAWESKTLTNQRKWSTLVHPADIDQTNY
                     VASIGNITQVYNEEQKFPEMDAYCISKIYADWIALGKTAETTALTTANILEVFDSLME
                     KMTEARVPAVGRILYVTPGVDTLIKNAKEIQRTVNIKDGGTSLNRQTTDIDSVKIVKV
                     PSNLMKTVYDFTTGWKAGAGAKQIFMSLIHPSAIITPVSYQFSTLDEPRAVTEGKYLY
                     FEESFEDVFILNKKADAVQFVVEA"
BASE COUNT         5226 a         3443 c         1942 g         7146 t
ORIGIN      
        1 tcataatctt ctttattatc acagaagaaa ctttcaatta atactgctgt aggctttgaa
       61 ctatttaaga tatataatct tttatctaat ttagcacctc tatttttaaa tactgtacct
      121 agtttatcac atattctagt tgcatactct aagcctttat tactataata taggacttct
      181 gaacctttac cttgaccgtt acttgcattt aaatgtaact ctataagtaa atcatatcct
      241 ccactattaa ctctaggtat tttataagac ttttcttcat tcttagtttt aaactgcttt
      301 tctgggcata ttattacatc taccttatgc ccttcttttc taaatgtatc tgctaatact
      361 ggtgcaagag atttgttgta ttgatactcg ttaattactc catcagcaga agtgcatgct
      421 ccacttttta aaatactgtg tcctactgta atacatattt tcattattta ctacctcctt
      481 taatatttaa ctcatctgtc atagtatcta ataagttacc tattctatct tttaattttt
      541 taggcactgg taacccacac aaatacatgt tttttaatat acttacactt tcatatagaa
      601 taaataaaat agagaaaaat tcagatattc caagatgatt taatcgtaaa aaatcaaccc
      661 aattctgcgg taacataaat aaaaaattaa actttgtaag tatgtcaact actgctagaa
      721 aaaatataca tgctatcatt gcaacttttc ttattcctcc atttattcca aaagagctat
      781 taaactgatg tgtttttatt gcccttaagc aacctagtaa tgtatcaaat gctattgcta
      841 atattactaa ttttataaat atatttgttg ctaaaaaaac tattgttacg ttcatatttc
      901 ctccttattt tgcattaaaa taagacttag aattatctaa gcctataaaa aagaacctat
      961 tttgtaggtt ctgttccttc tactactcca ctatgctcta taatataatc ctctactgct
     1021 tttctatact ctgtgttagt tacgtcatct aattcaaatt ctcgattttt tagagggttt
     1081 aaacctccac ttaaaatcct ctctgctaat attcttacta caatattatt tatgttcatt
     1141 ataaaattcc tcctactttt tcgttttctg caattaataa ttgattttct aattcttgta
     1201 ttcttttttc ttcttcattt acatatactg gtatttcttc tgaaattggt tcttttgttt
     1261 ctatatttat acctattatt ctatattttt catagtctat acttccatat ggaacatcaa
     1321 tacaatgcaa ctgtgttatt atatcatgct ccaatatatc ccctgttgct tctcctgttt
     1381 gcaagagtat tttacctgtt tggtcataaa ttattctatt tgctctattc attttatcac
     1441 ctcattttta atttattgta ttttaagagc ataccattta taagaagata gagtagaact
     1501 agactctgta aaggctggta ctctaatact tcctccagta atgtaaacat ctcttttatt
     1561 attttcatat aactttgaag caccactgtt tagagtacta gcactaaact cacaattaaa
     1621 tgatacattt aatacaaaat cagttagtat aactgtggat gaaggaaaat aagaagtggc
     1681 aataacataa tatttattcc atttatcact tgttgtgtat tcacactcag caataaaaac
     1741 attaggaaga aagtttagat tagtgatact aatccaatag ctaggattac ttgtgctgtt
     1801 taagtagaat gttttggcat ataaactagt attttttaca acagatgttg ttccagcagc
     1861 ataattagat atttttatat ttccaacttt atttattaga ctttgtatgc tctcagtttc
     1921 atttgcagaa acatttttat ttgttaaatt tactgctaaa gaattcttta atgtttctat
     1981 ttttgtttta gttgtctcta atttatctgt accaacaaaa ggattaccta atgcactaga
     2041 taggttattt ttaccagttt gaaaatcagt ttgtacactt tgcaatgctg tcataagctc
     2101 ccctaaacta gcattatcag ttaattttgt tgtcatattt tcacctcact tatatcatat
     2161 ctattagatt atttgctata ttaattcctt tttccctttg tccatttact tctgtatcta
     2221 acaagttttg ttttgttgct aattctttta atgctccttc tacattgtca ctttcaaata
     2281 agttatctgt atcttttata cttgttttct ctgctgttgt ttctatacta tctacactag
     2341 tttttacctc atttaatgca ccaacaagac tagttttatc tgttgtttca agtagagtag
     2401 tatcacctat tttgttgttt agctctgttt tagcaatttc tatgttgctt gttaattctg
     2461 tcttagttgt atcaatttta gtgtccaggt cctgcaaatc ttttaatgtg gcaaagatta
     2521 ttgttgggtc tatcttaagt tctatattat ttacattaga tacaacaaag atagttttta
     2581 ccttcatgtc aattactgct ccatcttcca ctttaggttt atatgctgac ctatactttg
     2641 atatagcaag taaattaccc tctgtatcta aataacctat ctctctaact ataaatcctc
     2701 cagcatttgc agggataaga ctctctaaaa ttagacaatt tgccatagct tcatcagttt
     2761 taacatttcc cacattgcct tcccaaacta cattcttaag tgctgtctgt tcttctgtag
     2821 ggttatactc atttcctcca ccatcaccta gttgaatttt agcaaagtca accttttcac
     2881 ctactaaact tgcatttgca atcttcgctt ttcctatgtc tgttaatatt gtgtagtaac
     2941 ttttatctgt agccaaacta ccacctccta tctaataaat tgtaatctct tggtatccaa
     3001 atccatttcc actcataatc ttaatctctc cttgtgtttc aatatcacta ggtgaccaag
     3061 ggtaaattgt aatctcatgc cctgtgactg ttgttgcacc aaaatacatg ccattgtctt
     3121 tactaactaa cactctagta taatcaagac tcatgttgca aggtttgatg ctacttacaa
     3181 atgtatgaac ctcattgaac caatcttgat ttctagcatc actctcaagg tgtatgttat
     3241 aagtagcatt attaatagtt aattcataat tgccttctcc aactacatta tctagccagt
     3301 tccttaaaaa tctctctgag taaggtaatt tacttatata tttactaaaa atcctaaacc
     3361 ttctatcttc taaactctca ttacttttag gagttataga cattatcttt tcccatcttt
     3421 ttataccact tatagttagg tcctctaaaa actggtcatt tgataggtcc tttaatttat
     3481 catgtagtgt ttttatttct ttattttcta cattaaatac ttttatatat tcttctttat
     3541 cttgtagaat ttgtggcaag taatttatta gattaatctc tttatccaac tacctcacct
     3601 ctcactacta tactgttact atctattgtt agattagatt taacatcatt tatcattgtg
     3661 tttgtaatat ctaacactcc atctatacta agtaatctag tttcaatttg agatatacgg
     3721 actattaagt tttcttcatc ttcccaactc atgttaagtt catttaaata gtcgtctatt
     3781 gcttcttctg caattgattt tatattctcc catgtatatc cactcttata tgttatttct
     3841 gctgatatat ttatagttgt acttgtaact ccttcaactg tgactctatg acctattggt
     3901 gctaatccta agccttgtcc ttgatgtcca attgggtcaa tttcttcttg cactaaatta
     3961 actaaatcct ctgatggtac tttgaaatta gaattaatta ttactaactt aacagtacct
     4021 ccaccgttcc aaactggata aactttaact cctccaatat cttgtatttt gttaacttca
     4081 tccttatagt tttgtatatt tccaccgaaa ctttgtgaat ttaaactatc atagtacctt
     4141 tgtcttaaac tgtcctcgct ctcttcatct tctccattta tcagtatttc tgttaactct
     4201 gctgtttcaa gaccgtctat atattcaatt ggaatcaact gaccaagttc aaaaataggt
     4261 ccagcagttt cacatttcat cttatatata ccttcagata ttctctcaat tgctacatag
     4321 ttatactctc ctaaattaaa cctagaatca agtggaatat ctatattgaa aacgccttta
     4381 gcaatcgtat tagtggctgg caaaggtgta attcctctct ctttacatct cttctctaaa
     4441 tagtaataac tagcagtatc tacaaatgtt tggtctagta attcatccat tgcaatgtat
     4501 gtttctgtaa gttctatagc aactggtgcc aaggcattat atattataga accttctctt
     4561 ttatcaaaag tatctggtac actatctaac attcttttaa ttatattttc aaatgtcatt
     4621 aactcaaaca attatacact caccaccttc tctgctttta tatttccata tttggtatgg
     4681 accataaatt tacaatggac ctttcctttt atattttgaa actcaaaatt atctacattt
     4741 tcaaccctat catcttgaat tagtgcttct ttgattcgtc tttcaagttc gggtattaca
     4801 aaggatatag gctctccaat aaggtcgttc aactcgactc cataatttct actgtatatt
     4861 aaatgctcat acctttcagt atttaaaatc aaaaagatgg tttgttttaa tgcttccaca
     4921 tcatcacaaa taccatctac tctgtttttt tctatatgaa gtttaaatgt cttacttggt
     4981 tcttgcctta catcaaaatt tataattgat acatcttcaa tatcatagtc aatgttatct
     5041 gttggtaaca ctttatcaca tcctatctaa aatcaagtat tgttgtcctc cttgcatacg
     5101 aattaaaacc aatttatccc ctattttttt atctgtatat cttttgaatg tatctgtttg
     5161 tattagaaaa aattcttcaa aagatgcttt ttgctctatc ttaactacta aaggattaac
     5221 actttctata gttccaaatg caatttgcat tggattactt gtttctactg catccattgc
     5281 tgctttcttg attatttgca ataattcttg gctcatttta tcacctcgct taaaagaatc
     5341 ttctagctct tgcaaaatca tgctttttct tttgtctacc acttaaacta cttattttta
     5401 ctacgtcacc agtttgtgga gcatgaatat attggtcatt tcctatataa agtccaacat
     5461 gatgtacttt tccttgttcg tttttaaaga atactaaatc acctgctttt acatcattta
     5521 tgttgcataa tagtttccct ctactatctt ttgattggtc tgctgaaact cttttgagat
     5581 ttattcctgc acctctttta aatgcccaca ccataagacc actacagtca aaactctttg
     5641 gaccattacc accccatttg taaggcttgc caagttgatt ttttgcttct tgaataactt
     5701 tacttacttt actattattg tttgttgatg tattagcatt gttactattt tgaacttgat
     5761 aagttgtatc ttttaaattc ttttctgctt cttcattgct cccaactcct gtacctgcac
     5821 tattagaact atatgcattt cctgttattt gtttataaaa tttgcctact cctggtaccc
     5881 aatctttatt aagaggacta tcataaagtg gagcatattt atctcttata ctttctaatg
     5941 ttttccttcc tatatgaata taatttcttg ataaattact tatacctctt tttattcctt
     6001 catctacaga gccaaaagac attcctttca ttccaaagaa attattttta gtatttgcaa
     6061 tatttgaagt ccctctagct gattcgtgca tagatatagc agccatgagt gctggattaa
     6121 ctttataagc atttgaatat ttaacaaata tatttcctgt atttgataat ttacctttaa
     6181 atactttatt taatttattt atcatgtcat tatcttcttt gcttgtagta ttttgtgcag
     6241 gaccattttt cttttcatct ttattatttg catttccact actgtagctt gaagaagaat
     6301 aagaagcaaa ttcatctcca tcaacaagag ttaaatccat gaaatgtgaa ttattttcaa
     6361 atgtatgttt tactttctca actaacatat aattttgtaa ctcaatatct cctaaatcta
     6421 aaaaaacagg tattaaacaa cctgctctta ctctaatatc tcctaacaca ttttttaaac
     6481 ttaatgactt agttttctta ttatataatt ttaaaagtat atcacacttt tgttttatct
     6541 ctgcttcact catgttttta tcaactgtct caaacatttg aagtattccc caacttctca
     6601 tatgtgctga gtcttgagca atatacacat ctctttttcc tgactcctcg ttgtctctga
     6661 cgagtttaat ttttgtataa gtatcactat ctatacttga attatagtca aaatcctcta
     6721 tgacatcatt attcataaca gtatctaatt tcattgatgc aacattcttt aatgttattc
     6781 ttccaaaatc atcatagagt acatacattt cctttttctc tcttagagta tcatctagtg
     6841 ctgttagtat catgtcaaat aaagttttat tttcttcaac tctcgatatt ttatattttg
     6901 tgtcttctat gacattgtat tttaagttaa aatctttagt tagcatcttt acaagttcac
     6961 ttgctgtttt attattatac acataagtat ctttattctt aaaatatctc aactggtcgt
     7021 aagcaacaat tttaatatga ttttctttat ctcttttctt ttgaaatata tatccataaa
     7081 atatacctat tcccttataa tacagcctta cagagttacc ttcacaaaac tctaatatat
     7141 catccatgac tattgtaaat tctaatttac ttggcgctcc tcgtctttct atctcccatg
     7201 tgattccatc taaaacgaca ggctcataga aatcttccca atgtgctatg actaatctta
     7261 catctctatc atttgctaac actaaatcat cagacaagcc tcaacacctg ccctttgtaa
     7321 atagtatatt tacttaaatt cttcccctta tttgccttat ccatcattga tttatttagt
     7381 tcatatactt tcttatatag tgaaccattt cctaattgct tttgacaaat tgcccaaaga
     7441 gaatcctctg cttttactgt atatgtttta gtgtttggtt cattaactga atcaacacgt
     7501 tttttctcta tctttacgtt tggtctacca gcatcactag tcgttttagg tgtagcagga
     7561 actaattttt tagttgagta atctctatat tgctttaact ttattgcaac ttttgtatct
     7621 gagccattct ctgcatcttc tactatgtta tattcctcta atgatacttt aatattagtg
     7681 ttaaatagta ctttattccc aagttgtcga gatacaataa attgaaatgg cttacaatct
     7741 gtctttagta gttctagttt acttaaaaag aattgaacat ctttaaaagc acctttgtaa
     7801 aacggtaatt tattatgtgt aaattctgct tcaaaactta tttcagatag cccttccttt
     7861 tttaatatgt ttacttctcc agtatttatc aaatcaactg tcttattctt gtttgttact
     7921 ttaatttcaa gtttaccagg tgcgattggt aattgtactc catctaaata aaagtcataa
     7981 gccatttata tccctcctaa actactccct cagcagatac aaccatagcg tcatttaatt
     8041 tttcagttaa aatattgact ataccatcca catctgcatc tttacttatg ttgtttgtat
     8101 tgttcatgtc aattttaatg tttactcctg tgaatcggtt tattacctcc tgctctgcaa
     8161 tatctctaag atattttaaa tcttcttgac ttttatccat tgttttagcc atttttgcag
     8221 tgtttcctgc cgtgtccttt acattttttc ctaaatctcc accaaatcct ccaagattat
     8281 taagtggatt gtttttatcc cacaaatctt ttaatcctag cttgtctttt gcatcttcta
     8341 gcatcttgtt aatgtcaaaa gtatctttta atttattaga tatagcattt tgccatttag
     8401 ttccaagtgc atttcctttt tgaaattctg ctccaatatc tttatattgc atcctttcca
     8461 attttatttt ttctggtgca tctcctaccc atctgtttaa actgttaatc tgttgcttaa
     8521 tgtaactgtt atccgcctta acaggtgtaa atgttgcctc tcctactttc ccaatgttaa
     8581 tcccagggat tttatttagc aaatctatta atttatttac acctcgtatt gctatattag
     8641 ctccagtaac aaaagcctta ccaagtgcat tacctgcatt attcacagag tcattcaatg
     8701 atgccatttt ctcaattata aagattacac cttttgcaat tgcttgtttc ataaaaaaaa
     8761 cacactgatt ccaaccattt gtaatgcctt catttacatc tacacatcta ttaaacagcc
     8821 atatcattat gttttgtata aatgctactg ctgcaaatac tgcacccaca attactccta
     8881 aggcggttaa attcgttcct gcgaatttgt ttactgctgc tactgcaaca aaaactacta
     8941 ctataaatgc tagaatgcct gcaactatcc aaaaaatcgg gcatgctaac atagcttcat
     9001 ttgctatcca ttgtccagca gcatatttta taagatacgc atatccagct aaagtttttg
     9061 tatttaaaag catctgagca aacatcatag caaatttaga tgctgcaact aatttttccc
     9121 ataaccacat tcctgccaaa gctaaagaat aaattgcaat agctgcgaca actccataaa
     9181 caatcggctc tattatgttc cagttctgtg caaaaacatt aacaacgcta agtgcagcat
     9241 tagtaattcc tccaagccct tgtattatta atactgctcc taatgtaatt ccttgaacaa
     9301 aatctgagaa aaaatcacta tttaatatat tttttataat atttaatgta tcataaatat
     9361 ttgaagatat tgcaatcatt acattccgca tagacacaac aatctcacta aatctaacgc
     9421 tttgcaaagc tccacttatt tttcctaatg tctgaccaaa tatcatataa gcatcatttt
     9481 tcatcattgt gagagcttga gaaaaagtga ttggcatact tgcaaatttc ttttctatct
     9541 catcagaagc tttaaacagt gcatttctta taacatctgc tgttattgct ccactacttg
     9601 ataattcctt caattggtct ttagttttcc ccattgcatc agctatttta gtagctagaa
     9661 gtggagcatt ttccatgata gaacgaaact catcaccttg aagtttccca gcacccatag
     9721 cttgagtaag ctgatacatt gctgcactag cttcacttgc tgatgtccct ccaattacaa
     9781 aagctttatt cataagttct gtaaacttta ccacctctgc tgaacttcca aaagcatctc
     9841 ctgctaatat cccaagttta gcaacctgtg ctgctgtatc tgcataacta gctcttgcac
     9901 tttgagcaga caaataaatc atcttattca gttgctctgt tgtctgtaag ccatcattca
     9961 ttagatttaa acgtgctttt gtactggcaa ttgtatctgc tgcttttgta atactttcta
    10021 tacctcttat tccaatatat acacccgcta agcttttaag tttagatact aatagagaac
    10081 cctcttcact tccttttctt attttattat taaagttttc ttgttcattt gtatttcttc
    10141 caattttctg ttctatcctc gttaaaatac tttcaatatt ttttaagttt tgttgtgaag
    10201 cttgtatatt acctgcattt ataggattgt tcaatttttg ttgtaatctc tctaaacttt
    10261 tagctgttgc attaatagat gtattcatat ttttaagtgc aggtgccatt ctatcaaaaa
    10321 tttttacaga tgcttgtatt ccaccttcat ctagaggatt tctcaattct cgttgtaatc
    10381 gctctaaact attaatcgtt gtgttaatgg atgtagtcat gtttctaaat gcaggtgtca
    10441 ttccgtcgaa aatctttatt gaagtttgta ttgtagccat ttcctcactc tcctttcttt
    10501 tttgaaataa taaaagcact tacttatttt taagtaagcg cttatgtatt ataaattcaa
    10561 tagttctttt ttcttaatat taaattcttc ttctgttatt gctcctaaat ctaataaatt
    10621 tttatatttt aatatttcat ctgcattaga attattgcta ttattttctg tatcttttaa
    10681 aattatagat aaaacagata aaatttcttg tgcatcttca tacgctcttt catatccaat
    10741 agaatttgtc tttgtttttc cactaattaa atttatatat gccacaggct ctgaaatagc
    10801 ttttaatgta agctttattt gtaaattttc aattatcttt tttgtttttt tcttgcctac
    10861 aacgctacca acaatagctc ctgtaccacc aaacaagact cctcctgcta aagcacttcc
    10921 aataccacct tttgttacag tctctccatc ttctagcaac tcaaaacctg ttatatcttc
    10981 aaaactataa acattcttct taatttttcc attttttctt ttagtttcta tatatatgag
    11041 tttattagtt tcgtcaaact ttataaaact gttaatttct ttagttacat taaatttttc
    11101 aactaaaaaa tcattttgtt tcttagaatc tagaacactt ttaatttcag attttgtagg
    11161 taatccatct ttaaatgtaa attgtttgtc tttgtattcc atataattca ttagacattc
    11221 tatgcaaact gaaccatcct ttattttttg gtctgtcttt tcttttccac atatacagca
    11281 cggttccttt gattttctaa ataatcccat aatattaccc ccatataata attttataag
    11341 attattatac tatataagta aaatttttac atcataatca cctcctttca ataaaaaaac
    11401 acctactatt caagtaagtg tttttagcgt ttttaatttt aagtccacat agttaatata
    11461 aaacattttt aattgaagtc caaatttgag aaaatgtgta atttacatac cacatagtta
    11521 atataaaata attatgggta gattttaaag ataaagtaaa aaatggattt acataccaca
    11581 tagttaatat aaaataaggt aatactgtaa tagaaccaga atttgattat ctatttacat
    11641 accacatagt taatataaaa tcccaaaata aacttagcat ttccaatacc tacacatacg
    11701 catctctctc aaatttgcag tgaaccatga gtagtgcaat tgataacatt tatcacacac
    11761 cctcaacgcc ttatatttca atgcttaagc cttaatttct tcaaaaaatc gaacactgca
    11821 aaatttctat gcctttatta tatcataaaa atattatttt tgaatatctg taccaatttg
    11881 tggtataata aaagcaagaa gaactacaat ctatttgaaa ctagagtgaa gttctaaaaa
    11941 ttaatgttta tttcttttaa acttgattgt aagtttaaaa tcaaagtcac tcttagtcgt
    12001 gtttgagtgg ctttttactt ttttaaataa cttactagtt aagtaaacta ttaaactggc
    12061 aactaaactt gctagtacac cttgtaaaaa attatccata catactcacc tcccttctat
    12121 acgttgggag gataatcttt tgtatgaact ccactctata aattgtagat tacatcttct
    12181 tgctaaaaat attataacat ataattctta catattttac ctatatttta tcttcttcta
    12241 cttcttctag cttctttagc agtctttttt tcttctttta tttcttcttc tactttaata
    12301 tctatagaag cagcaacaaa tgccctctcg tagtctggta aatctgtata ttcatgtggt
    12361 ttccatttga atttatgaag gcaataatga gctgcactag catcataatc gcctccttca
    12421 attagttttt tgcttcttct actttatcct caaaagtcct atcaaaacca tttacctctc
    12481 ctacttcact tgaaaggtct gtgtattcgc caggagttaa cattgttgtt aatagttcct
    12541 ctgctcccat tacaccatag ctattttgaa gttctgcatc atgtaaatct ggaaatacta
    12601 tagtttccac acataatttc aaagtgtaag tattaaaatc tgtttcacta gtgtattgcc
    12661 ctgttgcttt tccttttttg cctataacag gaactctaac agcagaattt tttctcaact
    12721 gtctatctct gtctgaatct attgctttaa gttcccattc tattggttta ccatcctcac
    12781 ctataaacct ttcacttgcc acatactttc tattctctac ttttattgca ttttgactta
    12841 aaaaagcgtt taaatctccc atattattct acctccatga ttaaatattt tgtttctttt
    12901 tctattgttg caatgccatc ctcatctagt ttgattttta ttgtaattgg ttttaatgat
    12961 gaaccttcaa ttgtatcaag tgccagttta tcagcaatat caactaaaaa tagacctgct
    13021 tttctataca tttctcctac acattcttct ataggtcttt tcttactgta atcaaaagaa
    13081 attgaatctt ttatctcata tttatcctta attttaacca tctcctattc ttataaataa
    13141 aaaatacaca tttatgattt atagatgtgt attttactcc ataccatttg ctatattgaa
    13201 cttctcgact attctccaat tctcaaaagt aaaatctaca tcctcatcca aatattctcc
    13261 atcagcgtca aatttagcaa ttatacctga atctaggtta caatcttcaa gtattatagt
    13321 ttgacgacct actgaacttg ttgggtcttc gttagtaatt tgcatatcaa agtaaatatc
    13381 ctcgcctgtt tctttatatt catataacaa ctctctaaaa atagaagtat tataataaaa
    13441 ggtagcactc cctgaaaatt tacttcctgt acttttattt ccttttgtaa tgctacctaa
    13501 aattggcatc tcacttttat ttttttccat cttagcttct aagttaatag cttgcataaa
    13561 attatatctt ttgcctttta ttgttacaaa acattcagct ttagaaccac ttattgcatc
    13621 tctaacattc atagtaatat ttttagacat ctatatcact ctcctttctt aactaactga
    13681 aacagtcata taaagcttac ccatagcact aataactttt acagcatcac ttactacaac
    13741 agttttcttg tcgcttccag gttctacaga aacatcatca gctttgaaat cttctattgc
    13801 tctcatattt tgcagttgtt catgatgctt aactacatca ttccaaaacg agatacgacc
    13861 agatttatca tttggtactt cgcccaagta ctttgtatta aataaagtcg ctatatcatt
    13921 agcaatctgg tcaagtactc taatactttg attagatgaa aaatcgtcat tcttatcgtc
    13981 tgtaaaactt acaaaagtat ttatgtcctc taatacatga acttcatctc ccaccttgtg
    14041 gaatataaat ttaccagttt ttaaagcttc ttcaagttgt atttgagtat aattaacatc
    14101 aacatcaaac tcaccatcat acttcttatt tgtattagat ttatttatgt cacatcctgc
    14161 tatagctcca gtagtccaat aaactaaact agattctaat aacccagtat ctttaatctt
    14221 attttctaca gatactacac cttcataatc tgcatcattt ttcttatata gtactgtttg
    14281 aaacttagct cctaccttat ctctcattct ctttgtaaat tctacaaaca aacttttaat
    14341 ttctgctgtt gtagccaaac accctagtgc attaaatgaa taactttcta ttttatccaa
    14401 gaaagcttgg tactctgctc ctgtcacagc ttcgccatta gttccaccag taaatacaag
    14461 tcctgcgctt gcttctaatg ttgcatcctt cttccaagtg atatagtcat tatcttgtaa
    14521 gtctgtaatc actttagcta tttgagtatc taccttctta ttatctaaaa gtgttacaac
    14581 atcaaactta gtgttatcat ctatatttgt tgttactata acttttaaat cattacctct
    14641 agtacctgag tacttagctg tagcaatact gcaactagct ttaacacctt tattcaattt
    14701 ataaaaatat cccaacctta tatttttaaa taaatctcta agacctttca acttatcatg
    14761 tgtataatca tatccaaaat acttagttga atacttctca aaatcatcac tggttacttg
    14821 aaatacttct tcatctatgc cccagtctag ctccaaaggc attgccacaa tacctctatc
    14881 cgataatgaa ctggttgccc tcttagctga gataaaattt atatatgcac caggtaggac
    14941 cttattttgc gctacaaatg ttcctccacc taaagccatc taactcactc ctttcataaa
    15001 attatttatt attttctcta cttctgagaa ggaatataac tcattttctc ttaaaattgc
    15061 atttaataaa tcttttctat ttacatactt cttagagtta actatctgct ccttagtaaa
    15121 tttgtaatca gtttctttgc ttaatgtctt actcaaaatt atcacctctc ttcaaaccac
    15181 cgaataactc tactgtatcc atcttatctg tatcattact ttttatagta aagtagttat
    15241 aatcaacgaa gaaatgaaga acattatcta caatttcaaa gttcatattt gtacctctaa
    15301 ctaaatctcc atcaatttct atatactcta attcctccaa taacatttca gctatctcat
    15361 ttatttcaaa attcttatct tttgaacgtg gtaagtaatg tacatcaaat gagtttttct
    15421 tcaactctct gccatttgga tataatgcct tacttgaatt tataggaaca ataaaaaaac
    15481 aaggttcatt tataccttgt tccacatctt cgctgtaaat tgtgtattca tttccaaacg
    15541 acttatctaa ctttactgat attccatcaa ttatattatt aatcatcaaa taccttcttt
    15601 aataaaacca ttaatttttt ctctattatt gcttctgctt cccctcttaa ttccattatt
    15661 gaaatagtca tcatatattt tcctttaaca aaacccttat gatttctagt tctatgacca
    15721 tattcaacaa aactggcata aggaactgga ttaatcacct ctataacata attatctcca
    15781 actttagtaa caggaagctt cctagcataa gctatcccac cccaaccatc ttttagagtt
    15841 ccagtctttt ttggtgtacg tccacgcttc ctagctttac gaacaagtct tgcagctaat
    15901 tctcttgcac aatcttttaa aaatttatca agctgactat cttctagcct ttgcaacttt
    15961 ctttgtagct tcttcaattc tttaaaatca acacttccac ctctagccat tacgctttat
    16021 cctctagtaa ttctaagatg atttcttgat ggtttggata tatggcagct tctccacttc
    16081 ttatatattc ttttgtaata ttattttgat tagttataat aagttttgaa cctgctttaa
    16141 tttctatatt tggagatata aagagtttaa tcgtctgtgt aagtttagcc acttttccat
    16201 ctgtagctga tgtagtattt ttatacgaaa gcttacaagg ttgattttct aatacaacta
    16261 cttctttgtt atttgttcgc tttgttatag ggtctttaat tggctgatac tcaactatag
    16321 tacatttatc tctatacatc atttctattg cttttctagt tctgtttacc atcttaaaca
    16381 cctaaaggtt aatattttat tcttaccata agttaaaaga taatttacaa gagtgtcaaa
    16441 gcgttgttct ggtgtttgag agccacttcc tatagcaaaa tctacctttg tatcaccttc
    16501 tgatatagac ttttctacag cttcaaaatt aagactttct atatctaatt gccccatatt
    16561 tttcttggta aataagaact ctccaactat catatcaact tcaatttctt tcaattcaat
    16621 tggcatagtt tttatattac aatctagttt aataatattt tctatttttt ctcttacaaa
    16681 acctattaac cacttatccc catcttttaa tatatattca aaactttcaa gtcttttttc
    16741 tatatcatca atcagattat tttccataat tttcacctac tttttagtaa gtttattttt
    16801 ctctttaagc tgcttatttt cttcttctaa agactcaact tttgacctta aaatattatt
    16861 ttcagctatt aaatctttta catttaatga cttgccatac tttactacct taccagtttc
    16921 atctatcaaa tcatatccca tttctaaaaa atcatctatt ctacattctt ctatagttaa
    16981 tattctattt aatttcttta cttgtggcat tatatatcac tcccttttct atgcttcaac
    17041 aacaaattgt acagcatcag cttttttatt taatataaat acatcctcaa aactttcttc
    17101 aaagtataaa tattttcctt ctgtaactgc ccttggctca tctaatgtag aaaattgata
    17161 agaaactggt gtaattattg cacttggatg tattaaagac ataaatattt gcttagctcc
    17221 tgcacctgct ttccatccag ttgtaaaatc atatactgtt ttcataagat tagatggtac
    17281 tttaactatt ttaactgaat caatatctgt agtttgtcta tttaaagatg ttcctccatc
    17341 ttttatgttt acagttcttt gtatttcttt tgcattctta atcaaagtat caacgcctgg
    17401 tgtaacatat aatattcttc caacagcagg aactctagct tctgtcattt tttccattag
    17461 gctatcaaaa acctctaaga tattcgctgt tgtaagagca gttgtctctg ctgttttacc
    17521 tagagctatc caatcagcat atattttaga tatacagtaa gcatccatct caggaaattt
    17581 ttgttcctca ttgtatactt gagttatatt tcctattgat gctacatagt tagtttggtc
    17641 aatatctgct ggatgaacca atgtagacca ttttctttgg ttagttaatg ttttagattc
    17701 ccaagcatta tcatagtttc tttgagctac tgctattgta tctctgtttg aatctac
//
LOCUS       DAMJYR010000077        17730 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.077, whole genome shotgun sequence.
ACCESSION   DAMJYR010000077 DAMJYR010000000
VERSION     DAMJYR010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 17730)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17730)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17730
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.077"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            106..489
                     /locus_tag="PC471_002483"
     CDS             106..489
                     /locus_tag="PC471_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HDF2789607.1"
                     /translation="MGFKKLPDSELKVMKFIWGLDGNKVASGEVVEAMKQIYDWNKNT
                     TLTILSKLAKKNYLYSQKTSKCTYYTIAVREKDYLKVETKKFFSFFHNNSLKSFFTAL
                     NDEENLSDDKLDMLEEWVKNWEEDE"
     gene            1168..2598
                     /locus_tag="PC471_002484"
     CDS             1168..2598
                     /locus_tag="PC471_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HDF2789608.1"
                     /translation="MILNQIFIEAGGIMRNKIIIGITFLLVLLMSGCASKQTAEINVL
                     ATTDLHGVIPYELTSYVKEERKKDKNLTLVDAGDFFDSGEVFGSSMDSYFSARYKDGD
                     NNTESYIETPISKDMKEVGYDVAVLGNHEFISNNKFYLDNMISDFEKQKINILSANTY
                     KKNGDSYTKPYIIKNINTPEGNVKLGILGLTIKEVGERKQWKDGKLVDSKSLELKDQE
                     GYNGELYMNDLVEDAKKWVSIMEKEKPDIIVAVAHTGEKPKKPKNPGNRIQDLAQQVD
                     GIDAIVAGHNHVQIEQHDYKNKSGENVIVTEPGKHGECISKINFKLEKNNDKWNIVDK
                     SSKLIKFEKNQESDKFGEFISSINKVSKDKKEIRLSEVKTPEWDKIYVFKSGTSADDI
                     YKTIGYKWRNISSPKSDGMVQIVVMNNNKPVSYIYGEAEVMPINIKCDESEYKDNVVT
                     IYPNKNDKFKIEKGKQEYSINLKHIQ"
     gene            complement(2746..3096)
                     /locus_tag="PC471_002485"
                     /pseudo
     CDS             complement(2746..3096)
                     /locus_tag="PC471_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904812.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            3091..3612
                     /locus_tag="PC471_002486"
     CDS             3091..3612
                     /locus_tag="PC471_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HDF2789609.1"
                     /translation="MFSIGGGYAIIPLIQEKVVNQTGWISEKIFTDIITISQMTPGPL
                     AVNTSTFVGLQIGGFWGAVAATLGCVLCGVVISLFLYNFFQKHQNSTYIFEILNGLKS
                     ASLGLIISAAATIIILALFGTNKVYFNSIFEMLNWTALIIFFVTLLIVRKWKINPIVI
                     IIMSGILGIFFYL"
     gene            complement(3741..3977)
                     /locus_tag="PC471_002487"
     CDS             complement(3741..3977)
                     /locus_tag="PC471_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2789610.1"
                     /translation="MLECFIAKDSKIKDGVNIPTLVFKTLHDRYFKTQYFDSYYNSLK
                     CKKKLVEINNVHNSYYIDSKKFCQEAYDWFVENQ"
     gene            complement(4287..7715)
                     /gene="uvrA"
                     /locus_tag="PC471_002488"
     CDS             complement(4287..7715)
                     /gene="uvrA"
                     /locus_tag="PC471_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HDF2789611.1"
                     /translation="MKINNYKNQSIITNPKKFENKYQDLPKTPIELLKVVQSLVIHGD
                     QGKLYGISFNKQQSDEELLRTIPQMLKRIFEINSNPLTIPRNPKQRLVGMCRDYSLLL
                     VSLLRYRGFEARMRAGFANYFESELTYEDHWLVEYYDTLTKRWIRIDAQIDDIQKNYF
                     QINFDTHDVGKTDGFLTGSEAWIRCRQGHAHPDDFGYNKNWKGWHSVKGNLLHDFNNM
                     IGLELLPWDLWTELSSKKYNQLTRAEKNLLDEMAEILSSGNIKIEALNLLIEKLPEDY
                     LKSIFSQLKILGISEIKELGNPLELEKKFKFTKAINKSIKNSLCHNKSSIYLKGGRQN
                     NLKDVEVTIPKNQITVITGVSGSGKSSLAFDTIYEEGKRRYFENLSNGAKLSEQLQKP
                     EFDLLQGLTPTIAIEQKKGSQNPRSTVGTLTSIWDYLRMLFVSIGKSYCPYCKIPLEK
                     KNNTKNYCPHCQTIFSKINTSTFNANSHTGACHDCNGLGFTYQVNPQLIVKDPTISIL
                     DGATYYFGKLRGKKPNGNWMVGELYAIAKDKNIDLDIPWNELPRDFIDAILYGTDDKI
                     YEFSFESKGRESKIRRPASGAINHIQRLFRESSSENNTLHQYMNKIPCNTCGGELLCI
                     EARFTTIKGYRFPELTKMTIEQLWNWLCELPNHLQKNELSLVNDILTELKIRVSYLLK
                     VGLSYISTDRTAPTLSGGELQRVRLSSQLGSELVGLTYILDEPSIGLHPRDHNLIIKM
                     IEELRDKGNTVIVVEHDKDTILSADYIIDVGPSAGTKGGFIIAEGTTQEIIKNPNSIT
                     GKYLSTYNKISSQNKIIPSKWLSLKGCHANNLKNIDVEIPLNCMCSITGVSGSGKSSL
                     VFHSLLPALEEKLKQKSIPDKNYTEFTGFDAIDDFILMDQAPIGKSSRSTPATYINIF
                     DEIRSLFAETPQAKQKLLDESYFSFNSKKGQCPNCQGLGKTKIILQYMADQWVTCSEC
                     QGKRYQKEILSIQYKGKTIADILDMEVAEAKTFFSDCSDIYRKLSLLDEVGLGYLKLG
                     QNTLGLSGGESQRIKLAKELGTKTKKRMLYILDEPTTGLHFKDIENLLITFRKLVNEQ
                     HSLLIIEHNTEVIRASDWIIDIGPDSGINGGEIVASGTPDEIKINPNSITGHFI"
     gene            8034..10268
                     /locus_tag="PC471_002489"
     CDS             8034..10268
                     /locus_tag="PC471_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HDF2789612.1"
                     /translation="MKLSRYKKNSLVLSIILCSLIISILVITVFNTVKLHNISNKQSK
                     NYLNDVSTQIVMNVDSKIKFILSDLRIMADFIKQYEGDSRYEYLSKRKTSYKYYDVGI
                     IDLEGKAEFLSGKKFDLKNTVSYKKGIEGKEYSEVIESMGFVLYSVPIFNDNHEVSSI
                     LIGVSNKESMNEILNIDNFNGKGTIEIINSKGKPLFIGKNTELIKDLSHKYKSSGDEP
                     WAQKMLEDFKNDKSGNITITSSKGVQCLLTYHPITRGLNDWHFLLIVPEDAVLGELNK
                     LNSFTITMTFIITCIIGIITLILYIIRRKYVNQIENIAYLDSITNGINSTKFSMLVKP
                     LISESPDSTYMMIAMNIKDFKLINDCFGSEKGNMTLKHLYNILYKNIDENEEFVCRHD
                     ADLFYLLIKNRPVLEALELLYKIEADTNYFNKNRENPYFLRLSVGIYTIENHNEDLIT
                     IQDHANTARKSFNKTHQSDFSFYSDIERKRLISEKEISNLMEKALENKEFFMCLQPKI
                     DIKSGKISGAESLVRWKNSEKGIIYPSDFIPLFEKSGFICKLDLYILEETCKLISKWI
                     KEGKEVLRISVNVSRQHLNDKLFLEKYKHICNKYNVPTCLIDLELTESIFLENPEAID
                     IIEDIHSNGFKCSIDDFGFGYSSLGILKDFKVDIIKLDRSFFVSKNNIDRGKVVIKSI
                     IELSKRLGMKVTAEGIEELEQVEFLKNVGCDYIQGYVFSKPLLISDFENFAYENNKIK
                     NIHY"
     gene            10524..12554
                     /locus_tag="PC471_002490"
     CDS             10524..12554
                     /locus_tag="PC471_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDF2789613.1"
                     /translation="MISGRMLLIMNFLKGKNKTSYKDISKQLSIEERKVRYDINNLNI
                     VLRSLNKSVIQKMNKGVLIIPDDFQVVCINNNEYVFSLTERVSIMKIISMFKIDNLNL
                     EKLSKEFQVSRSSIKNDLNVLSSELERNQITITYDKSFKISGEEDIIYKQRLNILKSY
                     SYLFGKEKSDLSVFEKYLVNVIEKIFKGIYLIDIYNWSMNLLLQMGWTLNDESFTWYV
                     SNIFLFTWYIHSDEKHPLESASLSEPCFENKFDQEFETIIQKKLTKEQIFLLKSFVFF
                     TNKYASLNEDMDLISTEIIVQDLIYNMSEILEISFKEDMILYKGLLNHIAPLIERIKQ
                     NIQIYEELLHVIPDKYQYVLEATKVSIQKNRVLSQIKNENETILLAIHFLGSIHRNER
                     NNYKNILLVCGFGYGVIAMLKDSLVNDYQINIVESIPSYKLQDYKEWESIDFIITTSK
                     IVIDLPKTIIEINPFLQEEDYLKFEEKGIKRKNVLTNYMSIKKRLDFLEEDAQKRVLS
                     IICQELGYSDERILFRQLNLSDLIGIDTIRIIDGDMKWEDAVKISSNILANCDFVSAQ
                     YAENIIDILKNVGFYAVKDNEFALLHGNDSSLVKVSSISLLICKEAVSFGDKKVKIIF
                     CLASRDKKEQIPAIINLTKMVYKTNFIAMLESAKTKEEAEVLIHKYEKEVTV"
     gene            12551..13003
                     /locus_tag="PC471_002491"
     CDS             12551..13003
                     /locus_tag="PC471_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDF2789614.1"
                     /translation="MNKLVNVKCSFKIHVNSKEEAIISLVDVVSKEGYLIDKNQFLKD
                     VLKREETLSTYIGHGIGLPHSQSVGVKNSCITIGKLDTPIEWTEEGEKVDLIFLISVT
                     KDNENNLHLKILSKLARLLMHESFRNQIRCSDEQTVYNLIKEKIEEED"
     gene            13005..14060
                     /locus_tag="PC471_002492"
     CDS             13005..14060
                     /locus_tag="PC471_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit EIIC"
                     /protein_id="HDF2789615.1"
                     /translation="MRILKFLQKHLMTATSYMIPFVVAGGILFALSVTLSGQAAVPET
                     GWLAKLNQIGAAGLALFIPILGGYIAFSMADKPGLAPGMIGAYLAKEVNAGFIGGIIA
                     GFIAGFVVLQLKKIKLAPTMRTLGSIFIYPLVGTLITGGIIVFLIGEPIASFMTWMTN
                     WLNGMSGVSKIPLGGILGGMIASDMGGPINKVAATFAQTQVDTLPYLMGGVGVAICIP
                     PIGLGLATLLFPKKFSKEERDSGKASLLMGCVGITEGAIPFATSDPVRVIPCIMAGSI
                     VGNIMAFLLGCLNHAPWGGLIVLPVVDNRLGYIASVLTGAVVVAVLMKLVKKDVKEDE
                     EIEEDLDESIELIFEEL"
     gene            14127..14435
                     /locus_tag="PC471_002493"
     CDS             14127..14435
                     /locus_tag="PC471_002493"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HDF2789616.1"
                     /translation="MKIVAVTSCPSGVAHTYMSAESLELSAKKFGIEIKVETQGSSGI
                     DNELSLKDIDEATCVILTNDVEIRNMERFKGKKVVRMSVSEIIKKSDALIKKIKDTFQ
                     "
     gene            14539..15804
                     /locus_tag="PC471_002494"
     CDS             14539..15804
                     /locus_tag="PC471_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II D-tagatose-bisphosphate aldolase,
                     non-catalytic subunit"
                     /protein_id="HDF2789617.1"
                     /translation="MKKLPIKTVVQRLLNLQEEGKSATLLGIGPMSPNLLQASFELAK
                     DDDFPLMFIASRNQVDADELGGGYVNGWNQETFTKDIKKVADKVGFDGLYYLCRDHGG
                     PWQRDKERNDHLPVDEAMELGKKSYLADIEAGFDLLMIDPTKDPFEIGKVIPLDVVLE
                     RTVELIEYCENERKRLNLPDIGYEVGTEETNGGLTSTETYETFITRLKVELDNRGLPM
                     PTFIVGQTGTLTRKTEQVGTFNFRNAYDLAQMAKKYGVGLKEHNGDYLDDVTLLEHIP
                     SQIIATNVAPQYGTEETRAYLKLAEVECKLEKEGLVEKTSNIRHVLLVNAIECGRWRK
                     WVVGEQKNLTTEEIFKDEVLSNEILDIAGHYTFNNDDVKKEIEVLYDNLSKNNIDGQR
                     FVVDHIKRPLRDYAECYNLKGVTTRILNK"
     gene            15892..16608
                     /locus_tag="PC471_002495"
     CDS             15892..16608
                     /locus_tag="PC471_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HDF2789618.1"
                     /translation="MINLIFDVDDTLYNQLTPFYTAYNKVFSSIKDISIEDLYMSSRK
                     YSDEVFHMTENGEMPIKEMHIYRIMKAFEELGNSITEKDAQSFQDEYIYQQSQITLIP
                     EVERILNFSKERNINLGIITNGPSNHQRMKLKQLNIENWVDKSNIFISSEVGFSKPDT
                     NIFRVAENVMNLDRENTYYVGDSYRNDVLGAKKAGWKSIWLNHRGHELEELFYKPDFV
                     ILEHKDLISLFIKICSYKNM"
     gene            complement(16803..17096)
                     /locus_tag="PC471_002496"
     CDS             complement(16803..17096)
                     /locus_tag="PC471_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat protein"
                     /protein_id="HDF2789619.1"
                     /translation="MRNMIENLIKNNTYIDDKVIMLSMLSSAKASANMYLNSALTSST
                     PELRAIYSASLTQMVEGHTALTELSINKGWVKPYDKPIQQLTCSYKESQNVID"
     gene            complement(17102..17317)
                     /locus_tag="PC471_002497"
     CDS             complement(17102..17317)
                     /locus_tag="PC471_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2789620.1"
                     /translation="MRILSETEKISLAAIIKMESDGLLMQRAINMLISDEDLKRQSES
                     SILATEGRIKAIQQFIVENEILISEEV"
BASE COUNT         6558 a         2049 c         2879 g         6244 t
ORIGIN      
        1 ggaaaagaat aatttatata tatattaatt atttttaaaa taaatttgtt ataatattta
       61 aatatacata aacatacata aatttacaaa aagggggtgt agcttatggg attcaagaaa
      121 ttgccagatt ctgagttaaa agtaatgaag tttatttggg gattagatgg aaataaggta
      181 gcatcaggag aagttgtgga agcaatgaaa caaatttatg actggaataa aaatacgaca
      241 ttaacaatat tgtctaagct tgcaaagaaa aactatttgt attctcaaaa aacaagcaag
      301 tgtacatatt atactatcgc tgtaagagaa aaagattatt taaaagttga aacaaaaaaa
      361 ttctttagtt tttttcataa taattcttta aaaagttttt tcactgcttt aaatgatgaa
      421 gaaaatttaa gtgatgataa attagatatg ttagaggaat gggtgaaaaa ttgggaagaa
      481 gatgaataaa atgaatgtga tactaaaatg atactaatat gttttatagc gtgttaaaaa
      541 atatagaaaa tatagaaata atagaagaaa attaatgtaa tagataaggt tttatcagat
      601 taaaaagaaa atcaatagaa tgggaataaa atatagaatg ccaaaaatcc acataaatac
      661 aggatttaag gtctctctta cttctatgtt atactggttt gatactatca atatttgcca
      721 gtatcttatg gattaataag taaaaaacat cctgctgaat gataaattca gcggggtgtt
      781 tttattttgt tttagaactc ctttgctctg aaactcgaag tatttaacac tgatgatttt
      841 gttatctttt agttcctttt gtgtcatgct ctgaaagaca aattgataca ataaaaaagt
      901 aatttcttaa tcattgatgg agatatgtat ttatgttact cttgatggta atttgagtaa
      961 tattgttcat agctttttat attcaattaa ttagaaaacc atttggtata atcataaata
     1021 aacttgatga tataatagaa aagatttata attattagaa gtatataaaa gtattttatg
     1081 tctatgaaat gtatgtctat aaaacatata gatgactttt gtatattaat gtatgaatat
     1141 tgatattata gaagaattga gttgtcaatg atactaaatc aaatatttat agaagctgga
     1201 gggattatga gaaataaaat aattatagga attacttttt tacttgtatt actaatgtct
     1261 ggatgtgctt caaaacaaac cgcagaaata aacgtattag caactactga tttacatgga
     1321 gttatacctt atgagttaac ttcttatgta aaagaagaaa gaaaaaaaga taaaaatcta
     1381 actttagtag atgcaggaga tttttttgat agtggagaag tgtttggttc tagcatggat
     1441 agttatttta gtgctagata taaagatggt gataataaca ctgaaagtta tattgagacg
     1501 cctatttcaa aagatatgaa agaggtaggt tatgatgttg cagttcttgg gaatcatgaa
     1561 tttatatcca acaataaatt ttatctagat aatatgatat ctgattttga gaagcaaaaa
     1621 ataaatattt tatcggcaaa tacgtataaa aaaaatggag acagttatac aaaaccatac
     1681 ataataaaaa atattaatac acctgaagga aacgtaaaac taggaatttt agggcttact
     1741 ataaaagaag ttggtgagag gaaacagtgg aaagatggaa aacttgttga ctctaagtct
     1801 ttagaactaa aagaccaaga gggctacaat ggtgaattgt atatgaatga tttagtagaa
     1861 gatgctaaaa agtgggttag tataatggaa aaagagaagc ctgacattat agttgctgtg
     1921 gcacatacag gtgagaaacc taagaaacct aaaaacccag gaaatagaat acaagactta
     1981 gctcaacaag ttgatggaat tgatgctata gttgcaggtc ataatcatgt tcaaattgag
     2041 caacatgatt ataaaaataa atcaggagaa aatgttatag ttactgagcc aggaaagcat
     2101 ggggaatgta tttctaagat taattttaaa ttagaaaaaa acaatgataa atggaatata
     2161 gttgacaaat ctagtaaatt aattaaattt gaaaaaaatc aagaaagtga taaatttgga
     2221 gaatttattt cttcaattaa taaagtaagt aaagataaga aagaaataag attatcagaa
     2281 gtaaagactc ctgaatggga taaaatatat gtttttaaat ctggaacatc tgcagatgat
     2341 atatataaaa caataggata taaatggaga aatatatcat ctccaaagag tgatggcatg
     2401 gtgcagattg ttgtaatgaa taataataag cctgttagct atatatatgg tgaagctgaa
     2461 gttatgccaa ttaatataaa atgtgatgaa tcagagtata aagataatgt tgtaactatt
     2521 tatcctaata aaaatgataa atttaaaatt gaaaaaggta aacaagaata tagtattaat
     2581 ttaaaacata tacaatagaa acttttatct tatttaagtt tagattgtac ttaaaataaa
     2641 gtgtgtatta ttttaaaaat tgatgataat taaaatcaca acattaagga atagtacaca
     2701 ctttttatgc taaaaaatag aatgttaaaa aattaaacct ttaaattaaa gtacattgct
     2761 tatattatcc aatattatct tcaaaggttg tgtaatattt ttatccttat gtaatattgc
     2821 taacatttta ttttccatat ctatcccatc taattcaatc aatcgcaatt ttttatgtaa
     2881 gagtgaatct aataataaat tttctggcaa aatggttata cctaaccctg cttgtgcagc
     2941 tttaataagt gcaaaagaat taacactttc ccaaacagga taagcttttt ggtttgaaag
     3001 agataataca ctgtccaaag tatcgcgaat ggcacttcct tgctcacgta acaataacgg
     3061 aaacgtgcat aactgtcgta gagataaggc ttgtttagta ttggtggtgg ttatgcaatc
     3121 atacctttaa tccaagagaa agtagtaaat caaactggat ggataagtga gaaaatattt
     3181 acagatatta taactatttc acaaatgaca ccagggccac tggctgttaa tacttctact
     3241 tttgttggac ttcaaattgg agggttttgg ggagcagttg cagccacttt aggttgtgta
     3301 ttatgtggtg tagtaatatc tctattttta tataattttt ttcaaaagca tcagaactct
     3361 acttatatat ttgaaatatt aaatggtcta aaatcagctt cattaggttt aattatctca
     3421 gctgcggcta caattataat tcttgcatta tttgggacga ataaagtgta cttcaattct
     3481 atatttgaga tgctaaactg gactgcacta ataatctttt ttgttacact actgatagta
     3541 cgtaagtgga aaattaatcc tattgtgatt ataataatga gtggtatact gggaatattt
     3601 ttttatttat aagtttttgc taagtaaagg atatctattt ttttatatct taaatccaaa
     3661 tagccaataa agtaaggcta tttggatttt taataaattc tgaaaagata atcagaaacc
     3721 tatataacat ttaaattata tcactggttt tctacaaacc aatcataagc ttcttggcaa
     3781 aatttcttac tatctatata gtaactatta tgaacattat taatttcaac aagtttcttt
     3841 ttacatttta gtgaattata atagctatca aaatattggg ttttaaaata tctatcatga
     3901 agtgttttaa agacaagagt aggtatgttg actccatctt ttatcttact atcttttgca
     3961 ataaagcatt ctaacattgt attaattatc tttgtactat ttttccaaat atgaggattt
     4021 ttcttttcaa atattttata aaattcatta tcatagactt cattataacc tttatattta
     4081 ggtggtttta ctgttatgtg aaaattagga agaaacttaa agaaaaaata taataatttt
     4141 tatgttctgt tttatatatt attcatattt tatcacagat aatatactaa agttaatttt
     4201 tttattgtac aactattata ataattcttc tataatataa gacattctat aattttatat
     4261 ttaagttcat gtgtatttaa ttgtttttat ataaaatgtc cagtaatact atttggattt
     4321 atttttattt catcaggagt tccagaagca acaatttccc ctccatttat tccactgtct
     4381 ggacctatat cgataatcca atcagaagca cgtataacct ctgtattgtg ttcaataatt
     4441 aaaagtgaat gctgttcatt tacaagtttt ctaaaagtaa ttaacaaatt ttctatatct
     4501 ttaaaatgaa gtccagtagt aggctcatcc aatatgtaca gcattcgttt tttagttttt
     4561 gtccctaatt cttttgctaa ttttatcctt tgagattctc ctccacttaa tcctaatgta
     4621 ttttgtccaa gctttagata tccaagtccc acttcatcta ataaggagag ttttctatat
     4681 atatcagagc aatcgctaaa aaatgttttt gcttctgcta cttccatgtc aagtatatct
     4741 gcaattgtct ttcccttgta ttgtatagac agtatttctt tttgatatct cttaccttga
     4801 cattcactac aagttaccca ttggtctgcc atatattgca gaataatttt tgttttacct
     4861 aatccttgac agtttgggca ttgccccttt ttgctattaa agctaaaata gctttcatcc
     4921 aataattttt gttttgcttg tggagtttca gcaaatagag aacgaatttc atcaaaaata
     4981 ttgatatatg tagcaggtgt agaacggcta gattttccaa taggtgcttg gtccattaag
     5041 ataaaatcat caatagcatc aaatccagta aattcagtat aatttttatc tgggatagat
     5101 ttttgtttca atttttcttc tagagcagga agtaaactgt gaaatacaag agagctctta
     5161 ccagaaccac taacacctgt aatagagcac atacagttta agggaatttc tacatcaata
     5221 ttttttaagt tatttgcatg gcatcctttt aagcttaacc atttacttgg tataatttta
     5281 ttttgtgaac ttattttatt gtatgtactt aaatatttac ctgttataga gtttggattt
     5341 tttataattt cttgggttgt accttctgcg ataatgaacc ccccttttgt accagcactt
     5401 gggcctacat caataatata atcagcagac agtattgtat ctttatcgtg ttccacaact
     5461 ataacagtat tacccttatc tcttagctct tctatcattt tgatgattaa attatggtct
     5521 cttgggtgta aaccaataga tggttcatct aaaatgtagg ttaagcctac taattcactt
     5581 cctagctgac ttgataatcg tactctctgc agttctcctc cagataaagt tggagctgtt
     5641 ctatctgttg atatatagct aagacctacc tttaatagat aactaactcg aatttttaat
     5701 tctgttaaga tatcatttac caaagagagc tcattttttt gaaggtgatt tggtaattca
     5761 cacaaccaat tccataactg ttctatcgtc atttttgtta gttcagggaa gcgataacct
     5821 ttgattgtag tgaatctagc ctcaatgcac agcaattcgc caccacatgt attgcaagga
     5881 attttgttca tatattgatg caaggtatta ttttcagaac tagattctct aaaaagtctt
     5941 tgaatgtggt tgattgctcc tgaagcaggt cttctaatct ttgattctcg tccctttgat
     6001 tcaaagctaa attcatatat cttatcgtct gtaccatata agattgcatc tataaaatct
     6061 cttggtaatt cattccaagg tatatctagg tctatatttt tatcttttgc tattgcatat
     6121 aattcaccta ccatccaatt accatttggc tttttccccc tcaattttcc aaaatagtaa
     6181 gtagctccat ctaatattga aatagttggg tcttttacaa ttaattgagg attaacttga
     6241 taggtaaaac ctaatccatt acaatcgtga caagctccag tatgggagtt agcattgaag
     6301 gttgaagtat ttatcttaga aaaaatagtt tgacaatgtg gacaataatt ttttgtatta
     6361 ttcttttttt ctaatggaat tttgcaatat ggacagtaac tttttccgat tgatacaaat
     6421 aacattctta aataatccca aatgcttgta agagtaccaa cagtagagcg aggattttgg
     6481 cttccttttt tctgttcgat ggctatagta ggggttaatc cctgaagtaa atcaaattcc
     6541 ggtttttgaa gctgttctga aagttttgct ccgttactta gattttcaaa ataacgtctt
     6601 tttccttctt cataaatcgt atcgaaagct aaagaagatt ttccactacc acttacacct
     6661 gtaataacag ttatttgatt ttttggaatt gtaacttcaa catcctttaa attgttttgc
     6721 cttcctcctt ttaaatagat agagctttta ttatgacaaa gtgaattttt aattgattta
     6781 tttattgctt ttgtaaattt gaatttcttt tctagttcaa gaggattgcc taattctttt
     6841 atttccgaaa tacctaatat ttttaactga gaaaagattg atttcaaata atcttcaggc
     6901 aatttttcta ttaacaaatt aagggcttct atttttatat ttccgcttga taatatctct
     6961 gccatttcat ctaataaatt tttttctgct cttgttaatt gattgtattt tttggaactt
     7021 aattctgtcc ataaatccca tggaagcaat tctaaaccaa tcatattatt aaagtcatgt
     7081 agaagattac cctttacaga atgccaacct ttccaatttt tattatatcc aaagtcatct
     7141 ggatgagcat gtccttgccg acatcttatc caggcttctg aaccagttaa aaaaccatct
     7201 gtttttccga catcatgagt gtcaaaatta atttgaaaat aatttttttg tatgtcatct
     7261 atttgtgcat ctattcttat ccatctcttt gttagagtat catagtattc tacaagccag
     7321 tggtcttcat aagtaagctc actttcaaaa taattagcaa aacctgctct cattctagcc
     7381 tcaaaacctc gatatcttag gagcgataca agaagtaaag aataatctct gcacattcca
     7441 acaagccttt gttttgggtt tcttggaata gttagtgggt tagaatttat ttcaaatatt
     7501 ctttttaaca tttgaggaat tgtacgaagc aattcctcat cagattgttg cttattaaag
     7561 gatataccat ataattttcc ttggtctcca tgaattacta ggctttgaac cacttttaat
     7621 aactcaattg gagtctttgg taaatcttga tatttatttt caaatttttt aggatttgtg
     7681 atgatacttt gatttttata gttatttatt ttcatttata tttcacctct ctaaatggat
     7741 gatataccct gacatggtgt catatgcaag gacaatttag atttttggcc aaaatttaaa
     7801 aaataaataa aaaatgatac agtgataaat tgtaatacaa atatagtttc aaattgcata
     7861 taatataatt atttaataaa accattatct attgaatgtt aatgaaatgt taagatggca
     7921 ttcttgaata ttaagaaata gaatactata atatagtgtg aaggattttt agtaaaaaaa
     7981 tttgtaagtg ttaatataca ttaaaaaata ggtttataat ttggaggaaa aaaatgaaat
     8041 tatcaaggta taagaaaaat agtttagttc tgtctataat tttatgttca cttattattt
     8101 caatccttgt aattactgtt tttaataccg taaaactaca caatatatct aataaacaat
     8161 ctaaaaacta tttaaatgat gtatctacac agattgtaat gaatgttgat tctaaaatca
     8221 aatttatttt atccgactta agaataatgg cagattttat taagcaatat gaaggtgata
     8281 gtcgatatga gtatttatct aaaagaaaga ctagttataa gtattatgat gttggaataa
     8341 tagatttaga aggtaaagct gagtttttaa gtggaaaaaa gtttgactta aagaatactg
     8401 tttcctataa aaaaggaatt gaaggtaaag aatactcaga agtaatagag tcaatgggtt
     8461 ttgttcttta tagtgtacct atatttaatg ataatcatga agtatctagt attttaatag
     8521 gtgtatccaa taaagaaagt atgaatgaga ttcttaatat agataacttt aatggaaaag
     8581 gaactattga aattattaat agtaagggaa aacctctttt cataggaaaa aatactgaat
     8641 taataaagga ccttagccat aaatataaga gttctggaga tgaaccttgg gcacaaaaga
     8701 tgcttgagga ttttaaaaat gataaatcag ggaatattac aataacctca tcaaaaggtg
     8761 tacaatgctt attaacttat catccaatta caagaggttt aaatgattgg cattttcttc
     8821 tcatagtacc tgaagatgct gtacttggtg aacttaataa attaaactct tttacaataa
     8881 caatgacatt tataattaca tgtattattg gaattataac attaatttta tatattataa
     8941 gaagaaaata tgtaaatcaa atagaaaata ttgcatattt agatagtata acaaatggta
     9001 taaattcaac taagtttagt atgctagtca aacctttaat ttcagaatca cctgatagta
     9061 cttatatgat gatagcaatg aatataaaag attttaagct aataaatgat tgtttcggta
     9121 gtgaaaaggg aaatatgaca ctaaaacatc tttacaatat tttgtataaa aacattgatg
     9181 aaaatgaaga atttgtgtgc cgtcatgatg cagacctttt ctatttgctt attaagaata
     9241 gaccagtgtt agaagccctg gagttattat ataaaataga agctgacaca aattatttta
     9301 ataaaaatag ggaaaatcca tactttttaa gactatcagt aggtatttac acaattgaaa
     9361 atcataatga agatttaatt acaatacaag accatgcaaa tacagcaaga aaaagcttta
     9421 ataaaactca tcaaagtgat ttttcttttt atagtgatat agaaagaaaa cgattaatat
     9481 ctgaaaagga aatttcaaat ttaatggaaa aagcccttga aaataaagaa ttttttatgt
     9541 gcttacaacc taaaattgat ataaaaagtg gaaaaattag tggagcagag tctcttgtgc
     9601 gttggaaaaa ttcagaaaag ggcataattt atccaagtga ttttattcct cttttcgaaa
     9661 aaagtggatt tatatgtaag ttagatttat atatactaga agaaacatgc aaattgattt
     9721 ctaagtggat aaaagaaggt aaagaagtgt tgagaatttc tgttaatgta tcacgtcaac
     9781 atctgaatga taaattgttt ttagaaaaat ataagcatat atgcaataaa tacaatgtac
     9841 caacatgtct gatagattta gaacttaccg aatctatttt cttagaaaat ccagaagcta
     9901 tagatataat agaggatatt cattctaatg gatttaaatg ttctatagac gattttggat
     9961 ttgggtactc atcattagga atattaaagg attttaaagt tgacataatt aagttagacc
    10021 gttcattctt tgttagcaaa aataatatag accgtggtaa ggtagttatt aagtctatta
    10081 ttgaacttag caaaaggcta ggtatgaaag taactgctga aggaattgaa gaattagaac
    10141 aagttgagtt tctaaaaaat gtgggatgtg attatattca aggttatgtt ttttcaaaac
    10201 cattattaat ttctgatttt gaaaattttg catatgaaaa taataaaatt aaaaatatcc
    10261 attattaaat gtacaagata aaaaatttag gcaagttata taattaaaaa tataacttgt
    10321 cttttttatt tatatattta ataaaaatat atttgcagat tttgtaaaaa taattttcaa
    10381 atgttgtaag ttgtggctaa agctttattg tattattctg aatatagaaa gatagttact
    10441 gtcttagata aaaactgaaa catacataat aatataaaat ttgctatatt aataactcta
    10501 attattttgg aaggagggaa aatatgataa gtgggagaat gttactcatc atgaattttc
    10561 taaaaggaaa aaataagaca agttacaagg atattagcaa acaacttagt atagaagaaa
    10621 gaaaagttag atatgatata aataatttaa atattgtttt gcgttcatta aacaagtcag
    10681 taatacaaaa gatgaacaaa ggtgtgttaa ttattcctga tgacttccaa gtagtatgta
    10741 taaataataa cgaatatgta ttttccttaa ctgagcgagt atctattatg aaaattatat
    10801 caatgtttaa aatagataat ctaaacttag aaaaattaag taaagaattt caggtttcta
    10861 gaagttcaat taaaaatgac cttaatgttt tgtcaagtga attagaaaga aaccaaataa
    10921 ctattactta tgataaatct tttaaaataa gtggagaaga agacattata tataagcaaa
    10981 gattgaacat attaaaatcg tattcttatc tttttggtaa ggaaaaatct gatttaagtg
    11041 tatttgagaa gtatttagtg aatgtaattg aaaaaatatt caaagggatt tatttgattg
    11101 atatatataa ttggtctatg aatttattat tgcaaatggg atggacttta aatgatgaat
    11161 cttttacatg gtatgtgtca aacattttcc tttttacatg gtatatacat tcagatgaaa
    11221 aacatccttt agaatctgct tcattaagtg agccgtgttt tgaaaataag tttgaccaag
    11281 aatttgaaac aattattcaa aaaaagttga ctaaggaaca aatttttctg ttaaagagtt
    11341 ttgtattttt tacaaataaa tatgcaagtt taaacgagga tatggatttg attagtacag
    11401 aaattatagt tcaagatcta atttacaata tgtcagaaat attagagatt agctttaaag
    11461 aagatatgat tctttataaa ggattactca atcatattgc cccattgata gagagaatca
    11521 aacagaatat acagatttat gaggaattac ttcatgtaat accagataaa tatcaatatg
    11581 tacttgaagc tactaaggtt tctattcaaa aaaacagagt tttaagtcaa ataaagaatg
    11641 aaaatgaaac aatacttttg gcaatacatt tcttaggaag tatacataga aatgagagga
    11701 ataactacaa aaatatattg ttagtttgtg gatttggata tggtgtaatt gctatgttaa
    11761 aggatagttt agtaaatgat taccagataa atatagtaga gagtattcca tcatataagt
    11821 tacaagatta taaagaatgg gaaagtattg acttcattat aacaacatca aagatagtaa
    11881 tagacttgcc taaaacaatt attgagataa atccattttt acaagaagag gattatttga
    11941 agtttgaaga aaaagggata aaaagaaaga acgtcttaac taattatatg tcaataaaaa
    12001 aaaggttgga ctttttagaa gaagatgccc aaaaaagagt attaagtatt atatgtcaag
    12061 aacttggata cagtgatgag agaatattat ttagacaact taatttaagt gatttaatag
    12121 gaattgatac cataaggata attgatggag atatgaaatg ggaagatgct gtaaaaatta
    12181 gttcaaacat acttgctaat tgtgattttg tatcagcaca atatgctgag aatattatag
    12241 atattttaaa aaatgtgggt ttttatgctg taaaagataa cgaatttgct ttactacatg
    12301 gcaatgatag ttcattagtc aaagtaagtt cgataagttt attaatatgt aaagaagcag
    12361 ttagttttgg agataaaaag gttaaaatca tattttgcct agcaagcaga gataaaaaag
    12421 agcaaattcc tgcaataatt aatctaacta aaatggttta taagactaat tttattgcta
    12481 tgttagaaag tgcaaagaca aaagaagaag cagaagtttt aattcataaa tatgagaagg
    12541 aggtcactgt atgaataaat tagttaatgt aaaatgttca tttaaaatac atgttaattc
    12601 aaaagaagaa gccattatat cgttagttga tgtagtgtca aaagaaggat atttaataga
    12661 taaaaatcaa tttttaaaag atgttttaaa gagagaagaa acattgtcga cttatattgg
    12721 acatggtata ggtcttcctc atagtcaaag tgtaggtgtt aaaaactctt gtattacaat
    12781 aggaaagctt gatactccta ttgaatggac agaagaagga gaaaaagtgg atttaatatt
    12841 tttgatatca gtaacaaaag acaatgaaaa taatcttcat ttaaagattt tatcaaaatt
    12901 agcacgttta ttgatgcatg aaagttttag aaatcaaatt agatgttcag atgaacaaac
    12961 tgtttataat cttataaaag aaaaaataga ggaggaagat taaaatgaga attttaaagt
    13021 ttttgcaaaa acatcttatg acagcaacat cttatatgat tccatttgtg gttgcaggag
    13081 gaatattatt tgctttatca gtaactttaa gtggacaagc tgctgttcct gaaacaggct
    13141 ggttggctaa actgaatcaa attggagctg ctggcttggc tttattcatt cctatattag
    13201 gtggatatat agcatttagt atggcagata aaccaggatt agctccaggt atgataggtg
    13261 catatttagc aaaagaagta aatgcaggtt ttataggtgg tatcatagca ggatttatag
    13321 caggatttgt tgttttacaa ttaaagaaaa ttaaattagc tcctacaatg agaactttag
    13381 gttctatatt tatttatcct ttagttggta cattgattac aggtggtata attgtatttt
    13441 taattggaga acctatagct tcttttatga catggatgac aaactggtta aatggaatgt
    13501 ctggtgtttc taaaattcct ttaggtggaa tcttaggagg aatgatagcc tcagatatgg
    13561 gtggccctat aaataaggtt gctgctactt ttgcccaaac tcaagttgac acacttccat
    13621 atttaatggg tggagttggc gttgcaattt gtatacctcc aattggttta ggattagcta
    13681 cattattgtt tccaaaaaaa tttagcaagg aagaaagaga ttcaggtaaa gcttcactat
    13741 taatgggatg tgttggaatt acagaaggag ctattccatt tgcaacatca gatccagtta
    13801 gagtaatacc ttgtattatg gctgggtcga ttgtaggaaa tattatggct tttttattag
    13861 gatgtttaaa tcatgcaccc tggggaggtc tgatagtgtt acctgtagtt gacaataggt
    13921 tggggtatat tgcatcagta ttaactggag cagtagttgt tgcggtatta atgaaattag
    13981 taaaaaaaga cgtgaaagaa gatgaagaaa ttgaagaaga tttagatgaa tcaattgaat
    14041 taatatttga agaattataa atataaatgg aaggaatttg tataaaaaag taaatataaa
    14101 actataaatt taaaggagat aagaatatga aaattgtagc agtaacatct tgtcctagtg
    14161 gagtagcaca tacttacatg tcggctgaat cacttgaatt atcagccaaa aaatttggta
    14221 tagaaataaa ggttgagact caaggaagct ctggaataga taatgaattg tcacttaaag
    14281 atattgatga agcaacttgt gtaatattga ctaatgatgt tgagattaga aatatggaac
    14341 gttttaaagg aaagaaggta gtaagaatga gtgtatctga gataattaag aaatctgatg
    14401 cattaattaa gaaaataaaa gatacttttc aataaaaatt gatttatttt acatataact
    14461 caaagatgtt tttaaagaaa tttttattaa aagcatcttt gaaaatatct taaaaataga
    14521 ataaaaggag atatagtcat gaaaaagtta ccaataaaga ctgtcgtgca aagattacta
    14581 aacttacaag aagaggggaa aagtgcaact cttttaggaa ttggtcctat gtcaccaaac
    14641 ttgttacaag caagttttga actagcaaaa gatgatgatt ttcctttaat gtttatcgca
    14701 agtagaaatc aagtagatgc agatgaacta ggtggcggtt atgtaaatgg ttggaatcag
    14761 gaaactttta caaaagatat taagaaggtt gccgacaaag ttggatttga tggtttatat
    14821 tatttatgta gagaccatgg tgggccatgg caaagagata aagagagaaa tgaccatttg
    14881 cctgttgatg aagcaatgga gttaggaaaa aaatcttatt tagcggacat tgaagcaggt
    14941 tttgacctat tgatgattga ccctacaaaa gacccatttg aaattggaaa agtaattcca
    15001 ctagatgtag tactagaaag aactgtagaa ttaattgagt attgtgaaaa cgagagaaaa
    15061 agacttaatc taccagacat aggttatgag gttggtacag aggaaaccaa tggaggtctg
    15121 acttcaactg agacgtatga aacatttata actagactaa aggttgaact cgataataga
    15181 ggtttgccaa tgccaacatt tatagttggt caaactggta ctttaactag aaagacagaa
    15241 caagtaggaa cttttaattt tagaaatgct tatgatttag ctcaaatggc taagaaatat
    15301 ggagttggtt taaaagagca taatggcgat tacttagatg atgtaacttt attagaacat
    15361 ataccatctc aaattattgc tacaaatgta gctcctcaat atggtactga agaaactagg
    15421 gcatatttaa aattagcaga agttgagtgt aaattagaaa aagagggctt agttgagaaa
    15481 acttctaata tcagacatgt tcttcttgtt aatgcaattg aatgtggacg ttggagaaaa
    15541 tgggtagtag gagaacaaaa aaatctaact actgaagaga tatttaagga tgaagtttta
    15601 tctaatgaaa tactagatat tgcaggacat tatacattta ataatgatga tgttaaaaaa
    15661 gaaatagaag tgctttatga taatttaagt aagaataata ttgatggtca aagatttgtt
    15721 gtagaccata ttaaacgtcc tttgcgtgat tatgcagaat gctataactt aaaaggtgtt
    15781 actacgagga ttttaaataa ataataggac ttatagtggt gagtattgtt atactaggta
    15841 tttatagtta taattatact taccactatt aaatttagga ggactaatat tttgattaat
    15901 ttaatttttg atgtagatga tactttatat aatcaattaa cgccttttta cactgcatat
    15961 aataaagtgt tttcttcaat aaaggatatt tctattgaag atttatatat gagtagtaga
    16021 aagtatagtg atgaggtatt tcatatgact gaaaatggag aaatgccaat caaagaaatg
    16081 catatttata ggataatgaa agcttttgag gagttgggaa attctattac agaaaaagat
    16141 gcacaaagtt ttcaagatga gtatatatat caacaatcac aaattacact aataccagaa
    16201 gttgagagga ttttaaattt ttctaaagaa agaaatatta atctaggaat tataactaat
    16261 ggcccatcaa accatcaaag aatgaagtta aagcagttaa atattgaaaa ctgggttgat
    16321 aaaagtaata tttttatttc tagtgaggtt ggattttcta agccagatac taatattttt
    16381 agagtagctg agaatgttat gaatttagat agagaaaata catattatgt gggtgattct
    16441 tatagaaatg atgtattagg tgctaaaaaa gctggttgga agagtatatg gttgaaccat
    16501 aggggacatg agttagaaga gttgttttat aaacctgatt ttgtaatttt ggaacataag
    16561 gatttgatat ctttatttat aaaaatatgt agttataaaa atatgtagta aaaaaatgtt
    16621 aataatagaa ttgtataaaa taattaataa taactatata aaattatatg tttagattgt
    16681 gagcaatcta ttttatgtta aaaatagatt atttagaaga aggggaaatc ttaaaaaaat
    16741 ttgaatatcc cctttttatt atttaattaa aatgaaatag ttagaaatat attttattta
    16801 gtttagtcaa taacattttg ggattcttta taactacatg ttaactgttg aataggttta
    16861 tcatatggtt taacccatcc tttattaata cttaattctg taagtgctgt gtgaccttct
    16921 accatttgag ttaatgaagc tgaatatata gctcttagtt caggtgtact acttgtcaat
    16981 gcagaattaa gatacatatt ggcagatgct ttagcagatg aaagcatact taacattatt
    17041 actttatcgt ctatataagt attgttctta attagatttt ctatcatatt tctcatgtta
    17101 tctatacctc ctcagaaatt agtatttcat tttcaactat aaattgttgt atagctttaa
    17161 ttcttccttc tgttgctaat atactagatt ctgattgtct ttttagatct tcatctgata
    17221 ttaacatatt tattgctctt tgcataagta atccgtcaga ctccattttt ataattgctg
    17281 ctaaagatat tttttcagtt tctgaaagta ttctcattta atcatctcct atattgtaat
    17341 ttatgcttta atagtatgtc tttcttttca aggaaataac cattacactt ttttacagta
    17401 ttttatattc tcgttatatc aaaatttagt cgacttaata tattaatata attttgtagg
    17461 atatttatca ttaaatatct ataaaataaa ttgtttgaat attcataaag caaaatttat
    17521 ttgtatgtac ttcttcaaga gtaaatatga gttttttcaa aaaattaatt tttatgataa
    17581 ttattaaggg ctatattttt cttaaagttg tgattatcat tattaattta caataaaatt
    17641 gccgaaaagc cctctccttt aggtgtaggg atgaaaggta aggtcttaat ataaaaaagt
    17701 tagaataatg cttgtaatct aataccgcta
//
LOCUS       DAMJYR010000078        17517 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.078, whole genome shotgun sequence.
ACCESSION   DAMJYR010000078 DAMJYR010000000
VERSION     DAMJYR010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 17517)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17517)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17517
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.078"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(208..435)
                     /gene="acpP"
                     /locus_tag="PC471_002498"
     CDS             complement(208..435)
                     /gene="acpP"
                     /locus_tag="PC471_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HDF2789621.1"
                     /translation="MFEKIKEIIAEQLGVDNLDEITMEASLMDDLEADSLDAVEIIMA
                     LEDEFGIEIPDEEAENFKCIGDICKYIEENK"
     gene            607..1032
                     /locus_tag="PC471_002499"
     CDS             607..1032
                     /locus_tag="PC471_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2789622.1"
                     /translation="MKYVGLLLTSVGMFLLIAVNFYYNSITLDMQRIEDYVMETNLIL
                     EDVAEKESYVSNEKEDYISRLMHVKKGIENSKTSFLIERYKEYKIKSIESLIYTISEE
                     KKDYLDEVDRYNKLGEKEINKLINKNFLEVTYLSITTYI"
     gene            1080..1286
                     /locus_tag="PC471_002500"
     CDS             1080..1286
                     /locus_tag="PC471_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2789623.1"
                     /translation="MTKLEDNMERFFGGCEGFFGNKMLIIIVIVFLLLCTDILEDLLC
                     DDNIWIWVILIVLLLFNFDDGCCC"
     gene            1468..1647
                     /locus_tag="PC471_002501"
     CDS             1468..1647
                     /locus_tag="PC471_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2789624.1"
                     /translation="MLDRFFGEEGCFGGGGWWIIVLFFLFLAFGDSWAEFDIMAWIPF
                     LIVLLILCCCGGCLE"
     gene            1681..1881
                     /locus_tag="PC471_002502"
     CDS             1681..1881
                     /locus_tag="PC471_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2789625.1"
                     /translation="MLDRFFGEEGCFGGGGWWIIVLFFLFLAFEDCWRDIDIMSWIPF
                     LILLLITCSCGGFFDGEVGCGC"
     gene            2134..3150
                     /gene="ccpA"
                     /locus_tag="PC471_002503"
     CDS             2134..3150
                     /gene="ccpA"
                     /locus_tag="PC471_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator CcpA"
                     /protein_id="HDF2789626.1"
                     /translation="MKGNITIKDVAKQAGVSISTVSRVINDSKPVTDEVKQKVLEVIK
                     ETGYIPNPLARSLVTKKSQLIGVIVPEVSDSFVNEVLNGIEEVAKMYDYDILLANTYS
                     DKEQELKSINLLRAKQVEGIVMISWIVEQEHINYIQNCGIPATYISKTARNYDIYTVS
                     TSNEEATFDMTEHLIKKGHEKIAFIMTSKDDTVLEMERLAGYEKALSNNNIELDKSLI
                     KYGGTDYESGYNSMKELLDDGIIPHAAFVTGDEAAIGAINAICDAGYKVPEDISVAGF
                     NDVKIARMYRPKLTTVYQPLYDMGAVAIRMVIKLINKELIENKKIELPYRIVDRESVT
                     ERKK"
     gene            complement(3273..3671)
                     /locus_tag="PC471_002504"
     CDS             complement(3273..3671)
                     /locus_tag="PC471_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2789627.1"
                     /translation="MKKLDTESLEKMAKQKNIDKDKIEKMADSYKGKSENELMEELIK
                     IGKNLDGRDEVVSKFKAFLDENQRKKLDNIMEKISDAENQRDTKPAKTKKAKPTKGKS
                     NSPAPKNTPGSQQKKSKSLFKKTKKSNPHE"
     gene            3883..4794
                     /locus_tag="PC471_002505"
     CDS             3883..4794
                     /locus_tag="PC471_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2789628.1"
                     /translation="MNNLLFLIGVIALSNGNISIFDDKNKSKGNNRVRKNKKNANDKL
                     SQSTHGNLRFDANDIKRGLRLMDLSEEDLEMGMEIITRTKKYMSRDERKILIKIESVL
                     DLVRGIKKLNNIDMVDTEDETDFFRKMDSDDKKNMMIKEIIDVFPEKRKNSVEKAIGM
                     KKKIDLFAELFLPDDFGEGGFSLSSLANINNLGSMNNLKLLGSLLRGDDSNNDDEDNN
                     DELNYEDEELDCEDKELDDDYDELTDEGEMEDNEDELVEDKNTGYENIENDEDIVYDE
                     ELYNINEQESEYNYNEKNTKTRAKKKR"
     gene            4909..5089
                     /gene="ssrS"
                     /locus_tag="PC471_002506"
     ncRNA           4909..5089
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="PC471_002506"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            5272..6489
                     /gene="cdeC"
                     /locus_tag="PC471_002507"
     CDS             5272..6489
                     /gene="cdeC"
                     /locus_tag="PC471_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exosporium morphogenetic protein CdeC"
                     /protein_id="HDF2789629.1"
                     /translation="MQDYKKNKRRMMNQPMSTMNEEEVYTDEINSEDMRGFKKSHHHN
                     GCNTDNKCECHDDCNPCNPCNPCKPNPCNPCKPNPCDDNCGCHDNCKCDCEPCEMDSD
                     ECFENKCGPECCNPISPRNFSVSNAVPFAIEANRIFDTMQFQTFTDATGPNGEPLTFE
                     TEVVEVFGSVPSAGQASVTIEKICLSNDGIVIDTGMTTLEDFDLDPLGDIVGRNCETT
                     FEFAVCGERNAECCRQGKGKSVAYKQRGLTVAVRNLVLELRGRCGCTEFVALAFPAVR
                     AGGGCKRRVDYVEFTFNTLSAPICLPADGRAVTLRQEYQTNLTVDCIGKSILKLECNE
                     CCEPFYELIIPNDIDLVLCLQETVSTLISEQIVVLASPNPIQPRLVDTFSKVCDFSQC
                     GPNHGSGKPSCHR"
     gene            complement(6590..7912)
                     /locus_tag="PC471_002508"
     CDS             complement(6590..7912)
                     /locus_tag="PC471_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HDF2789630.1"
                     /translation="MKVPYLVLSTVILFISNFIVRIFGFLYKIFLSRALGETGLGIYH
                     MIFNFLMICLAVTTTGIPTALSCLVAKRKALNDRHNTNALFISTLYISFFVALIISIV
                     ASFNSSFLSLKFLKDAKLNLFILAVCPAIVIITLSNVLRGYYYGIKNVKIPAIGQIIE
                     QIGKILFVFLLVMYINNKSMNCYIALLGISIGELSNIIFMLICLWRDSSFDNRYIISI
                     KDFYNSSMETLKMSIPITCNRMSNILLQSISSMMIPSRLALSGMTYQQSLSMYGIVSG
                     MVMPFIFLPFTVGSALIVNLIPTISQEMALRKRKSVIKKIKYSILLTLFVGILSSIFF
                     YFFGKDLCILVFKNTLAGEYLKAMFLVPLFMSLNQTLSGILHSIRKELASSINTITGM
                     LIQLIALYVFLPIPGLNIYAYIYTMTIVSIFTCLLHTIVLFKSLKSIR"
     gene            complement(7963..8757)
                     /locus_tag="PC471_002509"
     CDS             complement(7963..8757)
                     /locus_tag="PC471_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase HisJ family protein"
                     /protein_id="HDF2789631.1"
                     /translation="MFYDYHMHSSFSTDGKSTMEEMVKKSIELGLEEICFTDHVDYDV
                     YADDSFSIVYEDYFKSLETLQSKYKDKISIKKGIEFGVQTQLMDTYKKEAHQYPLDFI
                     ICSIHAIDTMDLYLGNYFKDKTQHEVYENYYLYLYNIVKNYKDYSVLGHLDLIKRYAP
                     YDTILDDRLFSDIIEETLKQAIYDGKGIEINTSCYRYNLPDLTPSKYILQMYKDLGGE
                     IITTGSDSHHISQVACEFDYIYSLLKNMGFKYVSKFNKLKPEFIKL"
     gene            8867..10312
                     /locus_tag="PC471_002510"
     CDS             8867..10312
                     /locus_tag="PC471_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HDF2789632.1"
                     /translation="MEKYRIDLNEKKSTKYIQIFNHIKELIINKELVEHEKLPPIRKL
                     SNFLQVNNTTIVKVYELLENEGYVYKIIGSGTFVSSLNSYKSGIDNRKNKIIVQREDL
                     IHFDNGNPSNDMFPIDSFKNAVNMALSKDGSSIFEYDEGLGSEELREKMVEYLSDGNI
                     KTTKENIQIISGAQQGIDIVCKGLISYSDVVFMEEPSYNGAIEVFKSRGAKIISIPML
                     DDGIDIGILKLKLEKIKPRLIYIMPNFQNPTGISYSTYKKKKLIELAEEHDFYIIEDD
                     FISDFVFESKDNRTIRSYDDKNRVVYIKSFSKILMPGLRIGIVEMPNELLKKVLWAKY
                     SSDISTPGLIQKSMYYYMNNFDWKNYLSHIEKIYTDKYKLAKNLINDKLSGKLKVRKS
                     CGGINFFLELPRGYTSQAFTNFMLNKGVSMLPGTYFFDNLVDDRFFRINIARPSMEEL
                     EKGISIISDNIDEFFCEYKNKLDIKSNKLFY"
     gene            10341..10550
                     /locus_tag="PC471_002511"
     CDS             10341..10550
                     /locus_tag="PC471_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 domain-containing protein"
                     /protein_id="HDF2789633.1"
                     /translation="MFDKKKLDRINELAKKNKEGILSADEIKEREILRKEYLENFRAH
                     FRSRLDSVKVVSPEEYEQYMKNNKN"
     gene            10570..11964
                     /locus_tag="PC471_002512"
     CDS             10570..11964
                     /locus_tag="PC471_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HDF2789634.1"
                     /translation="MNLKYEFKNAWDFERKENTIEQIMQYSSNYMEFLSKSKTERLSV
                     KEIIKLAKENNYISIDEAMEKGSISCGDKIYVINKEKAVALFVIGKNYIEKGMKIIGS
                     HIDSPRLDLKPNPLYQESNLGFFKTHYYGGIKKYQWTAIPLALHGIVILNDGTKVDIS
                     IGEEDSDPVFCVTDLLIHLAGDQMQKKLSEGISGEALNILIGNMPLEDEEKEPITANI
                     LKILNEKYNIVEEDLLSAEIEVVPAGKARDLGLDRSMVLGYGHDDRVCSYAAVKAILE
                     TEQPEFTSVALCVDKEEIGSKGNTGMHSKFFENTVAELIALEGDYCDIKVRRALANSK
                     VLSADVSAGYDPNFGEAYEKRNSAYMGNGVVLTKYTGSRGKSGCNDANAEFMSEVKRI
                     FNKGNVIWQTAELGKVDQGGGGTIAHILANQGAEVIDCGVGVLNMHAPHEIVSKVDIY
                     EMYKGYKAFFNINL"
     gene            12204..12956
                     /locus_tag="PC471_002513"
     CDS             12204..12956
                     /locus_tag="PC471_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDF2789635.1"
                     /translation="MDEKRQKKEMDLEVNSFVPLYQQLYDNIKKQIASGIYKPGDKLP
                     SEGDLCKEFNISRITVRNALNELVKEDILCKKRGKGTYVTIPERIEATCAGNSFTNSC
                     HRINAKPSTKIISVLIKKADKQVAEALSIELEEKVICIKRLRLIDEVPVIFEVDYFRI
                     DYMFLLKEELEDKSLMEVISNNISTLPKRVENIFEVKHSNKEYSDHLKCASNMPLLKV
                     RQSVYTENNDVLYYNEQFIRSDKYKYAVSAEI"
     gene            12982..13395
                     /locus_tag="PC471_002514"
     CDS             12982..13395
                     /locus_tag="PC471_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDF2789636.1"
                     /translation="MKKIILASHGDFSKGLLNAVKMIVGDLADCVSSYGLYPGQSASD
                     FALELEKTISEDKECEYIILSDLYGASVCTAMLRLTNLHNVRLFSGMNLNMVLELLTR
                     FSDELTVEDIDQLVEESRRGIQSVTLQIKEEEEVF"
     gene            13399..14142
                     /locus_tag="PC471_002515"
     CDS             13399..14142
                     /locus_tag="PC471_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane biogenesis protein"
                     /protein_id="HDF2789637.1"
                     /translation="MKKKGILSVFKSKKPIIAMIHLKGDTPEDIFERAKKEITIFEEN
                     GVDGIMLENYYGNYYDLERILEYVSKANLSIPYGVNCLNVDTMGFELATKYNASYIQV
                     DSVVGHVKPRDEATLEEFFKLQRSKCPAYLIGGVRFKYQPVLSENDVEEDLKIGMTRC
                     DAIAVTENATGQETSMEKIELFRKNLGDFPLVIAAGVTLENAKKQLKLGDMAIIGSYF
                     KDNYKDFGDVSVEHVKTFMDEIKKIREEL"
     gene            14142..14612
                     /locus_tag="PC471_002516"
     CDS             14142..14612
                     /locus_tag="PC471_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDF2789638.1"
                     /translation="MIKLVRVDHRLIHGQVAFTWTKFLSTDCILIASDDLLKDELRMA
                     GLKMAKPSNVKLVMKSIADSIKALNSGVTDKYNLLILCESVEDVYRLAKEVKAIKSIN
                     LGGTKSDDNRENISKAVHVSKDDIKMIKELDSEGVNVFVQLVPDDDATNVMKLI"
     gene            14629..15387
                     /locus_tag="PC471_002517"
     CDS             14629..15387
                     /locus_tag="PC471_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDF2789639.1"
                     /translation="MEFTQVILITLIAFFAYMHSFVGSTMHNRPIVVAPLVGLALGNL
                     HTGIVIGSTLELVFMGAFPVGASNPPDFVSGSIIATAFVILTGQDVSAAVVLAVPIAT
                     LVLLIDNFLMTVVLTWGAHIADRYAEEGNIEGVERVQLLFGIGNKLILAIIVGIGFSL
                     GVPVIEKILSFIPSYVTHGMDVAAGVIPAIGFAMLARMMLNKKTVAFLLLGFILVAYL
                     NITVTGVALFGLAIALIYVNFVGEKEVIVDDNEF"
     gene            15371..16213
                     /locus_tag="PC471_002518"
     CDS             15371..16213
                     /locus_tag="PC471_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HDF2789640.1"
                     /translation="MTTSSKKLETISPDSKITRKDFWKCFRRSLTLDSSWNYERMQNI
                     AYAYMMAPIIRRLYKDDKEKKSKALKRHLEFMSVTPHISTLLVGISGAMEEENAKNKE
                     FDANSINAVKSSLMGPVSGIGDSFFWGTLKLIAAGVGIALASQGNIMGPILFLLIINV
                     PHFIIRYICLDKGFKYGTQFFKDVSGSSIVSKVMEAASMLGLMVIGGMTASNVMLKLS
                     VNVGSGEWAEPIQTYLDQIMPCMLPAMIFGIMYWLLGKKVKTTTILISVMIICIVLAA
                     IGVV"
     gene            complement(16373..17278)
                     /locus_tag="PC471_002519"
     CDS             complement(16373..17278)
                     /locus_tag="PC471_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDF2789641.1"
                     /translation="MDFKQLEVFVAVAKHQSFSKAARELFLTQPTVSAHIQNLERELE
                     TVLINRSNKVITLTKSGEILYEHAIYILNNCKRAIYDIKEYSGKIEGIIDIACSSIPE
                     TYILPDFMKSFSMSYPDVKFSISHYDSQYAISEILNERISFGLVGSKINNPQIEYLDL
                     LDDELVLITPSDFKIDNKNNCIDIGELAYLNFIMRKEGSGTRNLILNTLSKNNFPVSK
                     LNVIAHVESNEAIKEMVRLGLGVSFISYISAIDYLNAGKIKCYKIKDVDFTRKFFFIY
                     SKKKTFSPLEDKFLNRLCEYFEIII"
BASE COUNT         6800 a         1966 c         3029 g         5722 t
ORIGIN      
        1 acaaaatcaa gatggaacta tagatataat aataaagaat gttacgtttt atgaagaaaa
       61 gtaatttata tacttagtat ataaattaaa tataagagcg tgtattttat atattgtaaa
      121 taaatgtaat ctagtctgtg cgaactctaa tataatttat tatgtagaag tttacacaga
      181 ctatttatat ttgtagacaa aacgatacta tttgttttct tcgatatatt tacatatatc
      241 accaatacat ttaaagttct cagcttcctc gtctggtatt tctattccaa attcatcttc
      301 aagggccatt ataatttcaa cagcatctaa tgaatcagct tctaagtcgt ccattaaaga
      361 tgcttccata gttatctcat ctaaattgtc tacccctaat tgttctgcta ttatttcttt
      421 tattttctca aacattttaa gcctcctaaa gcagttttaa ttaattttaa tgttagttta
      481 atataatatc aagaattttt caatatggaa tctataaaaa ataataaaat tttataaatg
      541 tagtaataaa gtattatttg atgaataaac tttattgata acaaatataa cttaggagga
      601 aatgctatga aatatgtagg actattacta acttcagtag gaatgttttt acttatagca
      661 gtaaattttt attataattc aataacttta gatatgcaac gtatagagga ttatgtaatg
      721 gagaccaatt taatccttga agatgtagct gaaaaggaga gttatgtatc taatgaaaaa
      781 gaagactaca tatcaaggtt aatgcatgta aagaaaggaa tagaaaattc aaaaacatcc
      841 ttcttgatag aaagatataa ggagtacaaa ataaaatcaa tagaaagttt aatctatact
      901 atatcagagg agaaaaaaga ttatctggat gaagttgata gatacaataa acttggtgaa
      961 aaagaaataa ataaactaat caataaaaac tttttagaag taacttatct gtctattact
     1021 acatatattt aattaaagag acaaaactta gtctcttaat gattcaagga ggattagtta
     1081 tgacaaaatt agaagacaat atggaacgtt tctttggagg atgcgaagga ttttttggga
     1141 ataaaatgtt gataataata gttatagtat tcttactatt atgcacagac attttagaag
     1201 acttattatg cgatgacaat atatggatat gggttatatt aatagtattg ttattattta
     1261 acttcgatga tggttgttgt tgctaaaaaa tattgattat taaataggta aaaccagtaa
     1321 cttatacaag ttatggtttt acctttttta gttcaaatta aaaaatttaa aaaaatagta
     1381 aaaatacatt agcaaaatgg aaaccaaatg catagtatat gttaggagcg aaaagctcaa
     1441 caaaatatca acaggaggta acgtataatg ttagatagat tctttggaga agaaggatgt
     1501 tttggtggag gaggatggtg gataatagta ctattcttcc tattcttagc ttttggagat
     1561 agctgggcag aatttgacat aatggcatgg ataccattcc taatagtttt attaatactt
     1621 tgctgctgtg gtggatgctt agaataagaa cacagttatt aaaaaaagga gggctttgtt
     1681 atgttagata gattttttgg agaagaagga tgctttggtg gaggaggatg gtggataata
     1741 gtactattct tcctattctt agcttttgaa gattgctgga gagacattga cataatgtca
     1801 tggatacctt tcttaatatt attgctaata acttgttcat gtggtggatt ctttgatggt
     1861 gaagtaggtt gcggctgcta atacttaaat cattagaata aaaaaatgct ggtattttat
     1921 accagcattt ttttatttta ttttttaata tgatttaaat taagaatgaa tattattaaa
     1981 tttgttgaat atagggtata atataaaata taatgtattt aaatgtactt gcttttatgg
     2041 taaaataaaa acttatataa atgtagttaa aataggaacc ttcaaaaatt tggttcagtt
     2101 ttagataaat ttagtaaaat aaggaggatg aagatgaaag gcaatataac gataaaagat
     2161 gttgctaaac aagcaggagt gtcaatatct actgtatcta gagttataaa tgattcaaaa
     2221 cctgtaactg atgaagtcaa acaaaaagtt ttagaggtta taaaagagac tggatatata
     2281 ccaaatccac ttgctagaag cttagtaaca aagaagagtc aattaatagg ggtaatagtt
     2341 ccagaagttt cagattcttt tgttaatgag gtgttaaatg ggatagaaga ggttgctaaa
     2401 atgtatgact atgatattct tttagcgaat acatactctg ataaggaaca agaacttaag
     2461 agtataaatc tattgagagc aaaacaagtg gaaggtatag ttatgatttc atggatagtt
     2521 gaacaagaac atatcaacta tatacaaaat tgtggaatac cagcgacata tataagtaaa
     2581 actgctagaa attatgatat atatacagta agtactagca acgaagaagc tacttttgat
     2641 atgacagagc atcttataaa gaaaggtcat gaaaagatag cttttataat gacgagtaaa
     2701 gatgatactg ttttagaaat ggaaagactt gctggttatg agaaagcact ttcaaataac
     2761 aatatagaat tagacaagag tttgattaag tatggtggaa ctgattatga gagtggatac
     2821 aatagtatga aagaactatt agatgatgga ataatacctc atgcggcttt tgtaacaggt
     2881 gatgaggctg ccataggtgc tataaatgct atatgtgatg ctggatataa ggttccagaa
     2941 gacatatctg ttgcaggatt taatgatgtt aagatagcta gaatgtatag acctaaactt
     3001 actacagtat atcaacctct atacgatatg ggagcagtag caataagaat ggttataaag
     3061 ttaataaata aggaattaat tgagaataag aaaatagaat taccttatag aattgttgat
     3121 agagaaagtg ttacagaaag aaaaaaataa aactattaaa atcaatcagg tagtgcctaa
     3181 ggcactacct gattttttat gtcaataata tcaaatttaa taatagatta actaaaaata
     3241 attatctaat tacttaaata gtttattttt atctattcat gagggttaga ctttttagtt
     3301 ttcttaaata aacttttaga ttttttttgt tgactacctg gtgtattttt tggtgcaggt
     3361 gaatttgact tacctttagt aggtttagcc ttcttagttt tagctggttt agtatctctt
     3421 tggttttctg catctgagat tttttccatt atattatcca gttttttgcg ttgattttca
     3481 tctaaaaatg ctttgaattt tgatactact tcatctctgc catctaggtt cttacctatt
     3541 tttataagtt cctccataag ttcattttct gattttcctt tgtaactatc agccatcttt
     3601 tctattttat ccttgtcaat atttttttgt ttagccattt tttctaatga ctctgtatct
     3661 aattttttca taaaaaaacc cctttctata aatatatatt gaattatatt catactattt
     3721 atatatagaa ataatattga agtggtgaat tgtgagtgat atataatata aataaaaatt
     3781 cttatagata atatatatgc actgagcttt aaaaatggaa cattaaaata tgaaaaacat
     3841 actttaatat agggcaatta tataatataa gggggaattg tcttgaataa cttactattt
     3901 ttgattggag tgattgcatt gagtaatggt aatatctcta tatttgatga taaaaataaa
     3961 tcaaaaggaa ataatagagt tagaaaaaac aaaaaaaatg caaatgataa actttcacag
     4021 tctacacatg gcaatttaag gtttgatgca aatgatataa aaagaggttt aaggctcatg
     4081 gatttaagtg aagaagattt agaaatggga atggaaataa taacaagaac taaaaaatac
     4141 atgtcaagag atgaaagaaa aatactaata aaaatagaaa gtgttttaga tttagttaga
     4201 ggaataaaaa aattaaataa tatagatatg gtagatacag aagatgaaac ggattttttc
     4261 agaaaaatgg atagtgatga taaaaagaat atgatgataa aggaaattat agatgtattc
     4321 ccagaaaaaa gaaaaaattc agttgaaaaa gctataggaa tgaaaaagaa gatagattta
     4381 tttgcagaat tatttttacc agatgacttt ggtgaaggtg gatttagttt gagctctttg
     4441 gcaaatataa acaatcttgg tagtatgaat aatctcaaat tgttaggaag tcttttaaga
     4501 ggtgatgata gtaataatga tgatgaagac aataatgatg agttaaatta tgaagacgaa
     4561 gaattagatt gtgaagacaa agaattggat gatgattatg atgagttaac agacgaaggt
     4621 gaaatggagg ataatgaaga tgaattagta gaagataaga acacaggcta tgaaaatata
     4681 gaaaatgatg aagatatagt atatgatgag gaactataca acattaatga gcaagaatcc
     4741 gaatacaatt ataatgaaaa aaatactaaa actagagcta agaaaaaacg ataattaact
     4801 aaatacataa agaaaaaagg aagaaaaagt aatgatagaa taagattatc tcaattacta
     4861 tataaaacca ctttaaaaaa ttcaaaattt attatataat aataatatga aatcctgaaa
     4921 catacgataa ggctttttta aattcaacct acaaatgaaa tacgggagac cgtgtctggt
     4981 aaattataac atgcctagtt agtttggacg ttaaaggtta ctggcaagtc acccacctgg
     5041 gagtgcctag ggtgtgaatt tacttagcca ccggtgtttc gggacaaaaa aagtgctact
     5101 atttcaaata gtagtacttt ttttattaat atgcattttt ctactaaata cgcctaatta
     5161 ttgataaatt caaaaagcaa gtaacaatta agattataaa agcatagtat taattaggct
     5221 tcaaaaagcc aaagacttaa taagaatcgc ttacagggag ggcaatgaaa aatgcaagat
     5281 tataaaaaaa ataaaagaag aatgatgaat cagccaatgt ctacaatgaa tgaagaagaa
     5341 gtgtatacag atgaaataaa ttcagaagac atgagaggtt ttaaaaaatc acaccatcat
     5401 aatggatgta atactgataa taagtgtgag tgccatgatg attgcaatcc atgcaaccca
     5461 tgtaatccat gtaaacctaa cccatgcaat ccatgcaaac ctaatccatg tgatgacaat
     5521 tgtggatgcc atgacaattg taaatgtgat tgtgaaccat gtgaaatgga ttcagatgaa
     5581 tgttttgaaa acaaatgtgg accagaatgc tgtaatccta tatctccaag aaacttctct
     5641 gtatcaaatg cagtgccatt tgcaatagag gctaatagaa tatttgatac tatgcaattc
     5701 caaacattta cagatgcaac aggaccaaat ggagagccat taacttttga aacagaagta
     5761 gtagaagtat ttggttcagt tccaagtgca ggtcaagcaa gtgtaactat agaaaaaata
     5821 tgcttaagta atgatggaat cgttatagac acaggaatga caactttaga agatttcgat
     5881 ttagacccat taggagatat agtaggaaga aactgtgaaa caacttttga atttgcagtt
     5941 tgtggagaaa gaaacgctga gtgctgtaga caaggaaaag gcaaatcagt agcttataaa
     6001 caaagaggat taactgtagc agttcgtaat ttagtactag agctaagagg tagatgtgga
     6061 tgtacagagt tcgttgcatt agctttccca gcagttagag caggaggtgg atgtaagaga
     6121 agagttgatt atgtagaatt tacttttaac acactttcag caccaatatg cttgccagct
     6181 gacggaagag ctgttacttt aagacaagaa tatcaaacta acttaactgt agattgtata
     6241 ggaaaatcta tattaaaatt agaatgcaac gaatgttgtg aacctttcta tgaattaatt
     6301 ataccaaatg atatagattt agtactttgc ttacaagaaa cagttagcac attaataagt
     6361 gaacaaatag tagttttagc atcaccaaat ccaatccaac caagacttgt tgatactttc
     6421 tctaaagtat gtgatttttc gcaatgtgga cctaatcatg gaagtggaaa gccaagttgc
     6481 cacagataga taggtaagat atatagacag tttaaattat aaataaaaat catctaagga
     6541 aggctaccaa agtgtataat ttacattggt agccttcacg tttttataac tagcgtatag
     6601 attttagtga tttaaaaagc actatagtat gtaatagaca agtaaagatt gatactatcg
     6661 tcattgtgta tatgtatgca taaatattaa gtcctggaat tggaagaaat acatataaag
     6721 ctattagttg aattaacatg ccagtaatag tatttatact tgaagctaat tcttttctta
     6781 ttgaatgtaa aatgccagat agagtttgat ttagggacat aaatagtgga actaaaaaca
     6841 tagcttttag atattctcct gcaagtgtat ttttaaatac taatatacat aaatcttttc
     6901 caaagaaata aaaaaatatt gatgatagta tgcctacaaa taaagtaaga agtatggagt
     6961 atttgatttt tttaattaca ctcttacgct ttcttagagc catttcttgt gaaatagttg
     7021 gtattaaatt tacaataagc gctgaaccaa cagtaaaagg taaaaatata aaaggcatta
     7081 ccatcccact gacaatacca tacatactta aagattgttg ataagtcatt cctgataatg
     7141 ctaatcttga tggtatcatc atagaactta tagattgcaa aagtatgttt gacattctat
     7201 tacaagttat gggaatagac atttttaatg tttccataga agaattataa aaatctttta
     7261 tgcttataat atacctattg tcaaatgatg aatctcgcca taagcaaata agcataaata
     7321 taatattact caactcaccg attgatatac ctaatagagc tatataacaa ttcatagatt
     7381 tattatttat gtacataact agtaaaaaaa caaataatat tttaccaatt tgttcaatga
     7441 tttgtccaat agcagggatt tttacattct ttatgccata gtaatatcct cttagtacgt
     7501 ttgaaagagt tattataact atagcaggac aaacagctag tataaataga tttaatttag
     7561 catcttttaa aaatttaagt gataaaaaag aactattgaa agatgcaact atagatataa
     7621 ttagtgctac gaaaaatgat atatataaag ttgatataaa taaagcatta gtattgtgtc
     7681 tatcgtttaa agcttttctc tttgcaacta gacaactaag tgcagtaggt atacctgttg
     7741 ttgtaacagc taagcaaatc atcaaaaagt taaaaatcat atgatagata cctaaacctg
     7801 tttcacctag tgctcttgat aagaatattt tatataaaaa accaaatatt cttacaataa
     7861 aattggatat aaataaaata actgttgata gtactaaata aggtactttc aaaaaaatca
     7921 cctccttatc ttttaaatat attcacaagg aggtgtaaaa atttataatt ttataaattc
     7981 tggtttcaat ttattaaatt tagatacata tttaaatccc atatttttta gtaatgaata
     8041 tatgtagtca aactcacatg caacttgact tatatgatgt gaatctgaac ctgtggttat
     8101 gatttcccct cccaagtctt tatacatttg aagtatatac ttagatgggg ttaggtctgg
     8161 aagattatat ctataacaag aggtatttat ctcgatacct ttgccatcat aaatagcttg
     8221 tttaagagtt tcttcaatta tatcagaaaa tagtctatca tctaaaatag tgtcataagg
     8281 tgcatacctt ttaatcaaat ctaaatgacc taagacagag taatctttat aattcttaac
     8341 aatattatat aaataaagat agtaattttc ataaacttca tgttgagttt tatctttaaa
     8401 ataattacca aggtataagt ccattgtatc tatagcatgg atagaacata ttataaagtc
     8461 caatggatat tgatgtgctt cctttttgta tgtatccatt aattgagttt gaactccaaa
     8521 ttcaattcct ttttttattg atattttatc tttatatttg ctttgaagag tctctaaact
     8581 cttaaaataa tcttcgtaga caattgaaaa agaatcatca gcatatacat catagtcaac
     8641 atggtctgtg aaacaaattt cctccaaacc gagttcaata gattttttta ccatttcttc
     8701 cattgtagat tttccgtctg tagagaaact tgagtgcatg tgatagtcgt aaaacataaa
     8761 tatttcccct ttttataaaa attttggtat tatatataca tactaaataa tatcaaatat
     8821 acataaaagc atcaataaaa tataaataca atgagcggtg ataaatttgg aaaaatacag
     8881 aatagattta aatgagaaaa aatcaactaa atatatacag atatttaatc atataaaaga
     8941 attgataata aataaagagt tagtagaaca tgaaaaattg cctccaataa gaaaactatc
     9001 aaatttttta caagtaaata acactactat agttaaggta tacgagcttt tagaaaatga
     9061 aggatatgta tataaaatta tagggagtgg tacttttgta tcaagtttaa attcttataa
     9121 aagcggtata gataacagga aaaataagat tattgtgcaa agagaagatt taattcattt
     9181 tgacaatggg aatccatcta atgatatgtt tcctatcgat tcatttaaaa atgctgtaaa
     9241 tatggcatta tctaaagatg gtagttcaat atttgaatat gatgaaggtc ttggctctga
     9301 agaattaaga gaaaaaatgg tagagtatct aagtgatgga aatataaaaa ctactaagga
     9361 aaatattcaa ataatatcag gtgctcaaca aggtatagat atagtttgta aaggacttat
     9421 aagttattca gatgttgttt ttatggaaga accttcttac aatggagcaa ttgaagtgtt
     9481 taaaagtaga ggtgcaaaaa taatatctat acctatgtta gatgatggaa ttgatatagg
     9541 tattttaaaa ttaaagttgg aaaaaataaa accaagactt atatacatta tgcctaattt
     9601 tcaaaatcca actggaatat cttactcaac atataaaaaa aagaaattga tagaattggc
     9661 agaagaacat gatttttata taatagaaga tgattttata agtgattttg tttttgaatc
     9721 aaaggacaat agaacaatta gaagttatga tgataaaaat agagtagtat atataaaaag
     9781 cttttcaaaa atacttatgc caggtcttag gataggaatt gtagaaatgc caaatgagtt
     9841 gttaaaaaaa gttttatggg ctaaatattc atcagatata tcaacaccag gtttaattca
     9901 aaaatcaatg tattactata tgaataattt tgattggaag aattatttga gtcatataga
     9961 aaagatatat actgataagt ataaattggc aaaaaacttg attaatgaca aattgagtgg
    10021 taaattaaaa gttagaaaat cttgtggagg aataaacttt tttttagaat taccaagagg
    10081 atatacatct caagcattta caaattttat gttaaacaaa ggagtgtcaa tgcttccagg
    10141 gacttatttt tttgacaatc tagtggatga tagattcttt aggataaata tagctcgccc
    10201 aagcatggaa gaactagaaa agggaatatc tataataagt gataatatag atgagttttt
    10261 ttgtgaatat aaaaacaagt tagatataaa aagcaataaa ttattttatt aaataaatag
    10321 aggtttttta ggaggaaaaa atgtttgaca agaagaaatt agataggatt aatgaattag
    10381 caaagaaaaa taaagaggga atcctttctg ctgatgaaat caaagaaaga gaaattttaa
    10441 ggaaagaata tttagaaaac tttagagccc actttagatc aagactagat agtgtcaaag
    10501 tagtttcacc agaagaatat gagcaataca tgaaaaacaa taaaaattag aacttagtag
    10561 gaggaaaata tgaacttaaa gtacgaattt aagaatgctt gggattttga aagaaaagaa
    10621 aatactattg aacaaattat gcaatattct agcaattata tggagttttt aagtaagtca
    10681 aagactgaaa gactttcagt aaaagaaatt ataaaattgg ctaaagaaaa taactatatt
    10741 tcaatagatg aagctatgga aaaaggaagc ataagttgtg gtgataagat ttatgttata
    10801 aataaagaga aggcagtagc actttttgta ataggtaaaa attatataga aaaaggtatg
    10861 aagattatag gaagccatat agattcgcca agattagatt taaaaccaaa tccactgtat
    10921 caagagagta atctaggttt ttttaagact cattattatg gtggaataaa aaaataccaa
    10981 tggacggcta ttccacttgc tttacatggt atagttatat taaatgatgg aacaaaagtg
    11041 gacatatcta ttggggaaga agatagtgac ccagtatttt gtgtaactga tttattgatt
    11101 catttggctg gagaccaaat gcaaaagaaa ctatcagaag gaattagtgg agaagcttta
    11161 aatatattaa ttggaaatat gcctttagaa gatgaagaaa aagaacctat aacagcaaat
    11221 atattaaaga ttttaaatga gaaatacaat atagttgaag aagatttatt aagtgctgaa
    11281 attgaagtag ttccagctgg taaggctaga gatttagggc ttgataggtc tatggtgttg
    11341 ggatatgggc atgatgatag ggtatgttca tatgctgctg taaaagctat actagaaact
    11401 gaacaaccag aatttacttc agttgcatta tgtgtagata aagaagaaat tggttctaaa
    11461 gggaatacag gtatgcactc aaagtttttt gaaaatactg tggctgaact gattgcttta
    11521 gaaggagatt attgtgatat taaagtaaga agagctttag caaatagtaa ggtattatca
    11581 gctgatgttt ctgctggata tgacccaaat tttggagaag catatgaaaa gagaaattcg
    11641 gcatatatgg gaaatggagt agttttaact aagtatacag gttcaagagg aaagagtgga
    11701 tgtaatgatg ctaatgctga atttatgtca gaagtaaaga gaatttttaa taaaggaaat
    11761 gtaatatggc aaactgctga acttggaaaa gtagaccaag gaggaggcgg aactatagcc
    11821 catatattgg caaatcaagg tgcagaggtt atagactgtg gtgttggagt tttaaatatg
    11881 catgcaccac atgagattgt ctctaaagta gacatatatg aaatgtataa gggatataag
    11941 gcatttttta atataaattt ataagtaaaa atataaatgt aataagtgaa taaaggctgt
    12001 tgtataaaca atttaatctg ttgtacaaca gtcttatttt tatgtaaaaa tagcaatttg
    12061 tcacactaga gaaaataaaa gaaaatactg tcaaattaaa aattatttaa aaaaaaattg
    12121 acaacgattt catcttgtta taatatgtaa taaagaactt gttatataat gttataaaaa
    12181 tgttacatag ggaggtgagc aaaatggatg aaaagagaca aaaaaaagaa atggatttag
    12241 aagtaaattc ctttgttcct ttatatcaac aactatatga caacataaaa aaacaaatag
    12301 catctggcat atataaacca ggagataaac ttccatctga aggagactta tgtaaagaat
    12361 ttaatataag tcgtataact gtgagaaatg cacttaatga acttgtaaaa gaagatatat
    12421 tatgtaaaaa acgtggtaaa gggacttatg taacaatacc agagagaata gaagcaacat
    12481 gtgcaggaaa tagttttact aattcttgtc atcgtattaa tgcaaaacca agtaccaaaa
    12541 taatctctgt tttaataaaa aaagcagata agcaagtagc tgaagcttta agtattgaac
    12601 tagaagagaa ggtaatatgt attaaaagac ttagattaat agatgaagta cctgttattt
    12661 ttgaagttga ctattttaga atagattaca tgtttttatt aaaagaagaa ttagaagata
    12721 aatctttaat ggaggttata tctaataata taagcacatt acctaaacga gtagaaaata
    12781 tatttgaagt aaaacattca aacaaagaat attctgacca tcttaaatgt gcaagtaata
    12841 tgccactatt gaaagtaaga caatcggtgt atacagaaaa taatgatgtc ttatattaca
    12901 atgagcaatt tataagaagt gataagtaca aatatgcagt atcagcagaa atataatcta
    12961 taatttagta ggaggatgag tatgaaaaag ataatattag catctcatgg agacttttca
    13021 aaagggcttt taaatgcagt aaaaatgata gtaggagatt tagcagattg tgtcagttcg
    13081 tatggtttgt accctggaca aagtgcttca gattttgctt tagaattaga aaaaacaata
    13141 tcagaagata aagaatgtga atatataatt ttatctgact tatatggagc aagtgtatgt
    13201 acagcaatgt taagattaac taatctacat aatgttaggt tattttctgg aatgaatttg
    13261 aatatggtat tagaacttct tacaaggttt tctgatgaat taacagttga agatatagac
    13321 caattagtgg aagaatctag aagaggaata caaagcgtga cattacaaat taaagaagaa
    13381 gaagaggtat tttagtttat gaaaaaaaag ggaatattat cagtatttaa aagtaaaaaa
    13441 ccaattatag caatgataca cttaaaagga gatactccag aagatatctt tgaaagagct
    13501 aaaaaagaga ttacaatctt tgaagaaaat ggtgtagatg gaataatgtt agaaaattac
    13561 tatggtaatt attatgattt agaaagaata cttgaatatg tatcaaaagc taatttatca
    13621 ataccatatg gagtaaactg tttaaatgtt gatactatgg gatttgaact ggcaacaaaa
    13681 tataatgcta gttatataca ggttgattct gtagtaggtc atgttaaacc tagagatgaa
    13741 gctactttag aagagttttt taaattacaa cgttcaaaat gtccagcgta tttaattgga
    13801 ggcgtacgtt ttaaatatca gccagtatta tcagaaaatg atgttgaaga agatttaaaa
    13861 ataggaatga caagatgtga tgctatagca gttacagaaa atgctacagg tcaagaaaca
    13921 tcaatggaaa aaatagaact gtttcgtaaa aatttaggag acttcccatt ggttatagct
    13981 gctggagtta cattagaaaa tgcaaaaaaa cagcttaaat taggagatat ggcaataata
    14041 ggaagttact ttaaagataa ttacaaagat tttggggatg ttagtgtaga acacgtaaaa
    14101 acattcatgg atgaaatcaa aaaaattagg gaggaattat aatgattaaa ttagtaagag
    14161 tagaccacag acttatacat ggacaagtag catttacatg gacaaagttt ttaagtacag
    14221 actgtatatt gatagcaagt gatgacttat taaaagatga attaagaatg gcaggactta
    14281 aaatggctaa accatctaat gttaagttag taatgaaaag catagcagat tctataaaag
    14341 cacttaactc aggtgttact gataaataca acttattgat actttgtgaa tctgtagaag
    14401 atgtttacag acttgctaaa gaggtaaaag ctattaaatc tataaacctt ggtggaacaa
    14461 aatcagatga taatcgtgaa aatatatcta aagcagtgca tgtatcaaaa gatgatataa
    14521 agatgattaa agagttagat tcagaaggcg taaatgtatt tgtacaatta gttcctgatg
    14581 atgatgctac aaatgtaatg aaattaatat aattttaggg ggatgaaaat ggaatttaca
    14641 caagttattt tgataactct aattgcattc tttgcatata tgcatagttt tgtaggttct
    14701 acaatgcata atagacctat agttgttgca ccacttgttg gtttagcact aggtaatctt
    14761 catacaggta tagtaatagg ttcaacatta gaattagtat ttatgggtgc atttcctgtt
    14821 ggggcaagta atcctcctga ttttgtgtct ggttctatta ttgccacagc gtttgtaata
    14881 ttaacgggtc aagatgtatc agcagcagtt gtacttgcag ttcctatagc aactctagta
    14941 ttgcttatag ataacttctt aatgacagta gtacttacat ggggagctca tattgctgac
    15001 cgttatgcag aagaggggaa tattgaaggc gtagaacgtg tgcaattatt atttggtata
    15061 ggaaataaat taatattagc aataatagta ggtataggat tttcattagg tgttccagta
    15121 atagaaaaaa tactttcatt tataccatca tatgtaactc atggaatgga tgttgcagca
    15181 ggcgtgattc cagcaatagg ttttgccatg ttagctagaa tgatgcttaa taagaaaaca
    15241 gtagcatttt tattattagg atttatattg gttgcttacc taaacataac agtaacagga
    15301 gttgcattgt ttggattagc aatagcttta atatatgtta actttgtagg agagaaggag
    15361 gtaatagtag atgacaacga gttctaagaa attagagaca atttcaccag atagtaaaat
    15421 aacgaggaaa gacttttgga aatgttttag aaggtcattg acattagatt catcttggaa
    15481 ctacgaacgt atgcaaaata tagcatatgc atacatgatg gcaccaataa tccgtagatt
    15541 atataaagat gataaagaaa agaaatctaa ggctttaaaa agacatttag aatttatgtc
    15601 agttactcct catataagta ctcttttggt aggtatatct ggagctatgg aagaagaaaa
    15661 tgcaaagaat aaagaatttg atgctaatag tataaatgct gttaaatcaa gtttaatggg
    15721 tccagtttct gggataggag attcattttt ctggggaaca ttaaagctta tagcagctgg
    15781 ggttggtata gctttagctt ctcagggtaa cataatggga ccgattttat tccttttaat
    15841 aataaatgtt cctcatttta ttatacgcta tatatgtcta gataaaggat ttaagtatgg
    15901 aactcaattc tttaaagatg tgagtggttc aagtatagtt tcaaaagtta tggaagcagc
    15961 ttcaatgtta gggcttatgg taattggagg tatgactgca tctaatgtaa tgttaaaact
    16021 tagtgttaat gtaggtagtg gagaatgggc agagcctatt caaacttact tagaccaaat
    16081 aatgccatgt atgcttccag ctatgatatt cggtattatg tattggttat taggaaaaaa
    16141 ggtaaagaca actacgatat taatttcagt tatgattatt tgtatagtat tggctgctat
    16201 tggagtagta taaataaaaa aatcccaagt tcatttgaac gagggatttt ttgactaaat
    16261 taaataaaaa agaaagatgg ttcataataa tttataagca tttatgtgat agttataatt
    16321 agcttattaa ttagttataa actcaaaatt aagcactact ttattaaagt tattatatta
    16381 taatttcgaa atactcacat aatctgttta gaaatttatc ttctaatggg gaaaaagttt
    16441 tctttttaga gtaaatgaag aaaaactttc tagtaaaatc aacatccttt attttataac
    16501 attttatttt acctgcattg agataatcga tagctgaaat gtacgatata aaggacactc
    16561 caagacctaa tctaaccatt tccttaatag cctcatttga ttctacatga gcgattacat
    16621 ttaatttact tactggaaag ttatttttac taagtgtgtt tagtattaag tttcttgtac
    16681 ctgacccttc ttttctcata ataaaattta gataagccaa ttctcctata tcgatacaat
    16741 tatttttatt atcaatttta aaatctgaag gagtgataag aacaagttca tcatctaata
    16801 agtctaggta ctctatttga ggattattga ttttagaacc aactaatcca aagcttattc
    16861 gttcatttaa aatttctgat atagcatatt gagaatcata atggcttatg gaaaatttta
    16921 catctggata actcatagaa aaactcttca taaaatcagg taaaatataa gtttcaggta
    16981 tggaactaca tgctatatct ataatacctt ctatttttcc tgaatattct tttatatcat
    17041 aaattgcacg tttgcaatta ttcaatatat atatagcatg ttcataaaga atttcaccag
    17101 attttgtgag tgtaataact ttgttgcttc tatttataag aacagtttct agctctctct
    17161 ctaaattctg tatgtgtgcg cttacagtag gttgagttaa aaaaagttct cttgcagcct
    17221 ttgaaaaact ctgatgtttt gcaacagcaa caaatacttc aagttgttta aaatccatta
    17281 tttaatcctc cttaaattat ctaaaactat tataacatat ttgataaatt aatagtcaaa
    17341 ggtgattttt aaaatagaaa acctttaaat attcatggag tttttgattg tttaggaata
    17401 atttggtata atgttgtaag gtacttacaa taaaaatatc gtaaattgaa ttttcaaaat
    17461 tcaactttaa agtgtttttt attacagaaa aattacaatt agcttcttaa tcttatt
//
LOCUS       DAMJYR010000079        17391 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.079, whole genome shotgun sequence.
ACCESSION   DAMJYR010000079 DAMJYR010000000
VERSION     DAMJYR010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 17391)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17391)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17391
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.079"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            116..2179
                     /locus_tag="PC471_002520"
     CDS             116..2179
                     /locus_tag="PC471_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDF2789642.1"
                     /translation="MTKIILSNYRCFGEEPTTIEINNLTGFIGHNSSGKTALLSALMK
                     LFGEKSSDRIIERSDFHIPESIDPDSISKNELYIEAQFEFPELERGKDSISIPVFFKN
                     MVVSEPGGKPYLRIRLEANWEKSSNPEGSIDSNVYYITSTTEEINPEDKRNIKRYELD
                     QIKMIYVPAIRRPDEQLKNVSGTIISRLLNGISWSEQTKARIKTKASETEEIFFEEKG
                     VESLKKSLETQWNSYHKDIRYSNPKINFNSTDLETILKKVEVTFSPTGTHRDYRVEEL
                     GDGLRSLFYLSLVNTFLEIEEEILKSINDGKEEDIFTLIPPVLTIVAVEEPENHISPH
                     LLGRVVEKLNFISTKENSQTILTSHSPAIVKRIEPTDIRYFRICKEYECTKVKSIILP
                     NEEKEAEQFKFVKESVLAYPELYFAKLVVLGEGDSEEIIIKQMIELKAESVDSSEISI
                     VPLGGRHVNHFWRLLNDLEIPFITLLDLDRERDGGGWGRIKYAIQQLINIGHDKNVLL
                     YTCEGISQDNEFKEMHTWDVTDTENMAKWIEMLKGYDVYFSAPLDIDFAMLQSFKDKY
                     ISALTSAEGPRIKDHGKIQDIVINEDAEDESLKAAYKDRIDSDIKSTLKQEGGDGSTY
                     TEGEKELMIWYSYFFLGRGKPTTHLSLFSNNTDIDFESFPYCLKEFVNSIIEKMIDDS
                     GGGRK"
     gene            2176..4041
                     /locus_tag="PC471_002521"
     CDS             2176..4041
                     /locus_tag="PC471_002521"
                     /inference="COORDINATES: protein
                     motif:HMM:NF024642.1,HMM:NF024754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HDF2789643.1"
                     /translation="MKRNWVSKEDWSPAAGFRLEVEAMNTVINDKNTLVVAGPGAGKT
                     ELLAQRACFLLETNTCRYPKKILAISFKKDAAENLKERLELRCGKELASRFESKTYDA
                     FAKEIVDRFRNSLDEAYRPLNNYNIAENRDVRRAFEIAGLNFRGNQTEFNKVYGNKLS
                     INKLPLHTDFVDKIFIDAWNVLLKGNKDTNFVSSLTFGMISRLAEYLIRTNRYIKKAI
                     EMTYSHVFLDEFQDTKSIQYDLVKACFFKTETIITAVGDGKQRIMLWAGARKSIFEDF
                     QNDFLSQKRTLIMNHRSAPRLVEIQKAMYNSLNEEAIDLKTNEKWNYEDGEVYLRLFE
                     DYIKEADILSEEIVELVETGIEINEICILVKQSVDIYSKEIINKLSERNIKARNEAMY
                     QEILKEDIIKTLVSLFRLSLSDRNPDDWDYIYDILGELYGIDEGYNPKILNDFITKVE
                     LMINDLKVNLNITDEKQFSDLVDSKVEYISYEKFASKFQKYKSKDYFDDLVDKFKKLV
                     WIEYNETKDWIKAIDNFEGKNSIPIMTIHKSKGLEYDTIFFVGLEDSAFWNFKNQPDE
                     DRCAFFVALSRAKRRIDFTFSLDRPAGFSNSQKHYCINEFYELFDNTEIVKKIEY"
     gene            4165..4979
                     /locus_tag="PC471_002522"
                     /pseudo
     CDS             4165..4979
                     /locus_tag="PC471_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728762.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            5004..5492
                     /locus_tag="PC471_002523"
     CDS             5004..5492
                     /locus_tag="PC471_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HDF2789644.1"
                     /translation="MYEKDYKQYIKLDIPIDEAKIKNRFEIRLKNERAYHAAVDLLAN
                     RDEEKTAFSIINRYIRFVDKDDEKIRSEWKTNDQWSYFIGEGRAKLKLTTNPEPYNIQ
                     KTLNWLSRQVAPTLKTIKRLDSLNNSTLIEGLIRYARLTDKHKKIIEQNSVNVEDIIY
                     DE"
     gene            5605..5766
                     /locus_tag="PC471_002524"
     CDS             5605..5766
                     /locus_tag="PC471_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2789645.1"
                     /translation="MAIAIIGVYLGFKREFSKLIGFLVVALIAVGLVFNTAGVKDILL
                     ELFNKIIGV"
     gene            6006..6233
                     /locus_tag="PC471_002525"
     CDS             6006..6233
                     /locus_tag="PC471_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2789646.1"
                     /translation="MDIVKVFANNVRKYRNKKGLSQEAFAEKAGLHRTYICAIERKKR
                     SIALDNVQKIADALEIETYLLFIEGEEKECL"
     gene            6230..9169
                     /locus_tag="PC471_002526"
     CDS             6230..9169
                     /locus_tag="PC471_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HDF2789647.1"
                     /translation="MKKLPIEKLIENLGFSGSKNLVNIDMLTKLDISKHDVKTLKVIN
                     PYAIYLVNSKPFILFIESSFEEKKLQQISKLIWNAQIPIAFICDSNSVKIFNGKALDL
                     KSYLIEEIYNQSIEDFSEESEFSYLKISDPLFWDQYTKQYTKTHLNDYLLDNITSLTN
                     KLKKKYKVKFATKLVLRLIFIRYLIDRGVDIAYKNFNHDIKASQKELLKITKNKVELY
                     ELFKYLKEKFNGNLFELNDEINNTCLTDEVFILLSDFLSGKISIDNGQMSLFSMYDFN
                     IIPVELISNIYEILLGKEIRNKDNAFYTPNYLAEYILDKTTLEFLKNNRQYKILDPSC
                     GSGVFLVNSFRRIIDMNLGDKLFCEDDKLLKELLLSNIYGIDINEDAIDVTIFSLYLT
                     ILDYKDPKILSEFKLPNLKGCNLIASDFFDDNKLESLKKINFDFIIGNPPWGSVSDGL
                     HLDYCNKNGYNDMQQNKEISRSFIFRAKDFSSRNTVCSFVVHSKILYTQKKPSKKFRE
                     FLLKKSQIFSVVEMSSVRKLVFKNADAPAAVISFKYNESEENLNNRIIYTSLKPNVFF
                     KLFNIIVIEKYDVKYISQKLLLENDWAWKTIVYGFSKDIDIIMNIKKKYQTVNEIILK
                     NSFVHGTGIQINGNESHDATHLKGKWYVDSKKGIDSFYVDINKGKELTQDKMRRPKAN
                     QQEIFIPPYVLLKKGVDTKTYRYRAAFSNDAFVYSDAVTGICGKKDNLTTLYCLTGML
                     NSSFYSYLNIMLGTSIGIEREQGFSTEIFTYPAIESDEIANLVVKIQEATKLEKTTMF
                     LNEKNSCKYIKQLDKLILKSFNLNDDKFIDYAINIQIPELTNSKSTNIYRKITKEDLI
                     NYSECFKEQFSSIYRRVGKSISISVYPKILNRFTVFELEVTDEKTDREIMVKAEADNN
                     MELFTKIIRYDYNDVFHQIRDIIHFGENSFYIIKPNCYKYWHPAIAEMDLADVLDQIL
                     SDTGGEE"
     gene            9169..9843
                     /locus_tag="PC471_002527"
     CDS             9169..9843
                     /locus_tag="PC471_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2789648.1"
                     /translation="MGGFKNSSLNFYLKQGLYEIIIDGLIDCCLLMSKSCIEQSRKIL
                     NHEEKIRTHLVENYLENDIERRKTSLSKQHLRFEVEAQENYDNKNESYEGRVDIKVIS
                     KDTLADRRAYYIIECKRIDGKETLNKKYITEGVQRFITEKYSSHYGKNIMLGFIVKDI
                     NIQSNIQKINSLNQNILYTYIDTDMLTVRSDSENNYAVCESKYKVKSKLLLLNHIFYN
                     FSSIIR"
     gene            10004..10420
                     /locus_tag="PC471_002528"
                     /pseudo
     CDS             10004..10420
                     /locus_tag="PC471_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003455547.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
     gene            10380..12827
                     /locus_tag="PC471_002529"
                     /pseudo
     CDS             10380..12827
                     /locus_tag="PC471_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005423199.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            12967..13389
                     /locus_tag="PC471_002530"
     CDS             12967..13389
                     /locus_tag="PC471_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2789649.1"
                     /translation="MRLEDIIDKESYEEKYDDLTVEIKKLSKEKKTLKASLINEKSIK
                     ARLREFRKILERNELLEKFDRYVFASIVDKVIVGVINEDGIVDPYKITFVYKTSFDDK
                     QDGHKFINKSPLETYSKDNNDNKKKCSYTNNETLRDCS"
     gene            13927..14961
                     /locus_tag="PC471_002531"
     CDS             13927..14961
                     /locus_tag="PC471_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YHYH domain-containing protein"
                     /protein_id="HDF2789650.1"
                     /translation="MFKKKLIAVAMSITMISVGSFSYAHSGRTDSSGGHRDNKNKSGL
                     GSYHYHCGGYPAHLHNNGGCPYTGGGSSSGTTTSVNNEEKQKRSVGEKGYNQGYEDGY
                     KGNYSSSNYSGDYSDTYESKYSEGYEKGKAKLEEEEKVAKETGYNLGLTGAKSNNTYE
                     KEVLKNAYDTGYSTGYNEYKTKKIEEYKAKGIEDSNKDKEKMTFEENIDSEFIDAYNN
                     AYDEIQEQLKNDYTTQGFESAIKGERFDTSTIGNVKYANWFKEGYDNGKVKLPKAKES
                     VYNQGYNEEDFSIPDEMKSIETKLKGVYDEGLEKREEEKSRNATYGVGAGTAAVVAGV
                     VYKKKKRKKI"
     gene            15048..15251
                     /locus_tag="PC471_002532"
     CDS             15048..15251
                     /locus_tag="PC471_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4236 domain-containing protein"
                     /protein_id="HDF2789651.1"
                     /translation="MGFNFRKSINLGGGLKLNLGKKSVGISAGVKGARVSVNSKGRKS
                     ATLSIPGTGISYTKTSTKKRKPK"
     gene            15422..16582
                     /locus_tag="PC471_002533"
     CDS             15422..16582
                     /locus_tag="PC471_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp27"
                     /protein_id="HDF2789652.1"
                     /translation="MKLSKILLAIGMSVSLLIASCPTSNALSSINSIKGTNKYETAGL
                     IADKQNYDIAILINSENGLSDGLSASGLSGAKNAPILLTKKDTIPSETLKRLEKVKEV
                     YLIGGKNSIGASVQKILSDKKISINRISGTDRIDTSHKVAKEIGRVSTVKKVILTNAF
                     KGEPDAMSVASIAVRDKSPIVLTDGNTTSFATDGLETYAIGGKVSMSDKLINETKATR
                     IGGVDRYDTNKQIINKFYNGSKEFYIASGTDLVYSLVGSTIAQKTPIVLVGNGSNKSI
                     LKNASKITAIGNIDDVVIKQCLNITNNVGDSNTGIVKDTNSNNTNKPSNEPSSNNPGL
                     TDKSTVYVTPNGKSYHLTKNCSTLKRSKTINKVTLKQAKSQGKIDPCNVCVK"
     gene            16841..16981
                     /locus_tag="PC471_002534"
     CDS             16841..16981
                     /locus_tag="PC471_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2789653.1"
                     /translation="MENLMMSIITGVIASYIYDKLKSHSERPTKSGWELNIKFQKSKS
                     SK"
BASE COUNT         7125 a         1684 c         3008 g         5574 t
ORIGIN      
        1 ttattcgtat aataatagaa atattttagc ataagatgta taataaagga aaaaatacag
       61 aattaaaaaa cataagatag ataagtggac ttaggggaga gaaattatta tgcaattgac
      121 aaaaataatt cttagtaact ataggtgttt tggagaagaa cctacaacta ttgaaattaa
      181 taatttaact ggatttatag gtcataatag cagtggtaaa actgctttat taagtgcact
      241 tatgaaactt tttggagaaa agtctagtga tagaattatt gaaagatcag atttccatat
      301 acctgaaagt attgatccag atagtatttc taagaatgaa ttatatattg aagcacaatt
      361 tgagtttcct gaattggaga gaggtaaaga cagcatatct ataccagtat tttttaaaaa
      421 tatggttgtt agtgaaccag gtgggaaacc ttatttaaga attagacttg aagcaaattg
      481 ggagaaaagc tcaaatcctg aaggaagtat tgattcaaat gtatactata taacatctac
      541 aacagaggag ataaatcctg aagataagcg taatatcaaa agatatgagc ttgatcaaat
      601 aaagatgatt tatgtacctg ccattaggag accagatgaa cagttaaaaa atgtctcagg
      661 aacaataatt tcaagattat taaatggtat aagttggtca gaacaaacaa aggcaagaat
      721 taaaacgaaa gcaagtgaaa cggaggaaat tttctttgaa gaaaaagggg ttgaatcatt
      781 aaagaaatca ctcgaaactc aatggaatag ttatcataag gatattagat attctaatcc
      841 aaagataaac ttcaatagta ctgatttaga gacaatttta aaaaaagttg aagtaacttt
      901 ttcacctaca gggacacatc gagattatcg ggttgaagag cttggagatg ggttacgttc
      961 tttattttat ctttcacttg taaatacatt tttagaaatt gaagaagaaa ttctaaaatc
     1021 tattaatgat ggtaaagaag aagatatatt tacattaata ccgcctgtat taacaattgt
     1081 tgcggttgaa gaaccagaaa atcatatttc acctcatctt ttaggaaggg tagttgaaaa
     1141 attaaatttt atttctacaa aagaaaattc acagacaata ttgacatcac attcaccagc
     1201 aatagtaaaa agaattgaac caacagacat aaggtatttt aggatttgta aggaatatga
     1261 atgtacaaaa gtgaagtcaa ttatattacc aaatgaagaa aaggaagcag aacaatttaa
     1321 atttgtaaag gaatcagtat tagcatatcc tgaactttat tttgcaaaac ttgttgtatt
     1381 gggcgaaggt gatagtgaag agatcattat taagcagatg attgaattaa aagcagaatc
     1441 tgtagattca tcagaaattt caatagtacc attaggtggt aggcatgtta atcatttttg
     1501 gcgtttatta aatgatttag aaattccatt tattactttg ttagatttag atagagaacg
     1561 agatggaggt ggttggggac gtataaagta tgcaatacaa caattaataa atattggtca
     1621 tgataagaat gtattacttt atacatgtga aggaatttca caagataatg aatttaaaga
     1681 gatgcatact tgggatgtaa ctgatactga aaatatggct aaatggattg aaatgctaaa
     1741 aggttatgat gtttattttt cagctccgtt agatatagat tttgcaatgt tacaaagttt
     1801 taaagataaa tatatttcag cattaacaag tgcggaaggt cctaggataa aagatcatgg
     1861 aaaaatacaa gatattgtga tcaatgaaga tgctgaagat gaatcattga aagctgctta
     1921 taaagataga atagatagtg atattaaatc aaccttaaaa caagaaggtg gtgatggatc
     1981 tacttatact gagggagaga aagaactaat gatatggtat tcatatttct ttttaggaag
     2041 aggtaaacca actactcatt tatctttatt ttctaataat actgatatag attttgaaag
     2101 ttttccatac tgtctaaagg agtttgtaaa tagtattatt gaaaagatga tagatgattc
     2161 agggggtgga agaaaatgaa gaggaattgg gttagtaaag aagattggag tccagcggct
     2221 ggattcagac tagaagttga agcgatgaat acagttatta atgataagaa tacacttgtt
     2281 gtagctggtc ctggcgctgg aaaaacagag ttattagctc agagagcctg ctttttacta
     2341 gagactaata cctgtagata tcctaaaaaa atccttgcta ttagttttaa aaaggatgct
     2401 gctgaaaatt taaaagagcg tttagaattg agatgtggga aagaattagc ttcaagattt
     2461 gaatcaaaga cctatgatgc ttttgcaaaa gaaatcgtag atagatttag aaatagttta
     2521 gatgaagcat atagaccttt aaataattac aatatagctg aaaatcgtga tgttagaaga
     2581 gcatttgaga tagcaggatt aaatttcaga ggaaatcaaa ctgaatttaa taaagtatat
     2641 ggaaataaac tatcgataaa taaattgcct ttacatacag attttgtaga taagattttt
     2701 atagatgctt ggaatgtact acttaaaggc aacaaagata ctaattttgt aagttcactt
     2761 acttttggta tgatatcacg tttagccgaa tatttgataa gaaccaatag gtatattaag
     2821 aaagcaattg aaatgactta tagtcatgtg tttttagatg agtttcaaga tactaaatct
     2881 attcaatatg atcttgttaa agcttgtttt tttaaaacag aaacaataat tactgctgta
     2941 ggggatggca aacaacgaat tatgttatgg gctggtgcta gaaagtcaat atttgaagat
     3001 tttcaaaatg attttttaag tcaaaaaaga acattaatta tgaatcatcg ttcagcgcca
     3061 agacttgtag agattcaaaa agcaatgtat aacagtttga atgaagaggc aattgattta
     3121 aaaactaatg aaaagtggaa ttatgaagat ggagaagtgt atttaagact ttttgaggat
     3181 tatataaaag aagctgatat acttagtgag gaaatagtcg aattggtgga aacaggtata
     3241 gaaattaatg aaatatgtat attagtaaaa cagagcgtag atatttattc aaaagagatt
     3301 ataaataaac tttctgaacg taatattaag gcgcgtaatg aagcaatgta tcaggagata
     3361 ttaaaagaag atataataaa gacactggtt tcgcttttta ggttgtcgtt aagtgataga
     3421 aatcccgatg attgggatta tatttacgat attctaggtg aattatatgg aatagacgaa
     3481 gggtataacc ctaaaatatt aaatgatttc attaccaaag tagaattaat gattaatgat
     3541 ctcaaggtaa atttaaatat tactgatgaa aaacaatttt cagatttagt tgattctaag
     3601 gttgaatata tttcatatga aaaatttgca tcaaagtttc aaaaatataa atctaaagat
     3661 tattttgatg atttggtaga taaatttaaa aagctggtat ggattgaata taatgaaact
     3721 aaagattgga ttaaagcgat tgataacttt gaaggtaaaa attcaattcc aataatgaca
     3781 attcataaaa gtaaaggact tgaatatgat actatctttt ttgttgggtt agaagattca
     3841 gcattttgga attttaaaaa tcaaccagat gaagatagat gtgcattttt tgttgcatta
     3901 tcaagagcta aaagaagaat tgattttaca tttagcttag atagaccagc aggattttcg
     3961 aattcacaga agcattattg tattaatgaa ttctacgaat tgtttgataa tactgagatt
     4021 gtaaaaaaga tagagtattg attaagatga taaaaacttt agtatattat attttaagat
     4081 attagttata taagtagaca ttgaatttat gtatttgatg tctatttttt atataaaaat
     4141 atattaaaga aaggatgaat ttgcatgaag aaaaaaacaa aaatatctaa aaagattgta
     4201 agttttatta tggtttttat aactatagtt actactatac ttacatcatc agtttcagct
     4261 tatgctagcg agttggatat gagtgaacag acaggcttta attatttagg tattagtcca
     4321 attacaggaa aaaaattaat cataacattt atcgtatgaa gatggataat aaaattgtat
     4381 tttgtgtaga gccaggaatt aaaacttcaa gtggaggagg atatatatcg gaggagttag
     4441 gagataaatt tatatctaca acacttccaa attatcataa aagaaaagct gaaatacttg
     4501 agaaggtaaa tagacataat atattacctt catggaataa tgaaaaagtt gcagttaaaa
     4561 gtggagaaac aattgagtta gaagataaga atggtattat tagagaaatg caattagaat
     4621 caaataatac tggaacagat atattacttg aagaaaatac tttgaaaatt acagcaaata
     4681 aaaattctaa ttcaggagtt attagttata taaagattcc taaaaatgag gttggaatat
     4741 caatagttta tcgtaagcat aactatcaaa gcttatcaga atttcatatg gaaagttcga
     4801 tgagtgcaaa tttaaatata aatgtaatca agttaggaat tgttaaagta aaaaagattg
     4861 atgaaggcac aggaaaggaa ttagcaaatg caaaacttag atttgagtat gatggtgata
     4921 gtaaagaaat tataacgggt tctaatgaaa ttgctagtat agaagatatt attggttgat
     4981 taaaaagtga agtatacttt tgtatctatg aaaaagacta taagcaatat ataaaattag
     5041 atatacctat tgatgaagca aaaataaaaa atagatttga aataaggtta aaaaatgaaa
     5101 gagcatatca tgcagcagtt gatttgttag caaatagaga tgaagaaaag acagcatttt
     5161 caattattaa tcgttatata cgttttgttg ataaagatga tgaaaaaatt agaagtgaat
     5221 ggaaaactaa tgatcagtgg agttatttta ttggtgaagg tagagcgaag ttaaaactaa
     5281 caacaaatcc agaaccatat aatattcaaa aaactttaaa ttggttatca agacaagttg
     5341 caccaacgtt aaaaacaatt aaaaggttag atagtttgaa caactcaaca ttaattgagg
     5401 gcttaataag gtatgcaaga ctaacagata aacataaaaa aataattgaa caaaattcag
     5461 taaatgttga agacattatt tatgatgaat aaaaaaatgt aattagtatt gcagatttta
     5521 atataaaaga agggagatat acgaatgaat tttggtcaaa atttatataa ttagttttta
     5581 tcaaatgcac aatcattggt attaatggct atagctataa ttggtgtata tttaggattt
     5641 aagagagagt tttcaaaact tataggattt ttagtagttg cattaattgc agtagggctt
     5701 gtgtttaata cagcaggagt taaagatata ttattggagc tatttaataa aataataggt
     5761 gtgtaaggat taaaagagaa tagaaaatta tgagtatatg atgaagggtt ctaataagtt
     5821 ttattaaaat atatattgaa agacaagtag agctatttcg agttattaca tgtaatgcta
     5881 atatatataa ctgcattttg gaaattaaat acataattag aaatagcaaa ttttataggt
     5941 gacttattaa tatgtttact tattgtatac tatttgatat atttaatatg aggtgattct
     6001 tgaaaatgga catagtgaaa gtttttgcta acaatgtgag aaaatataga aataaaaaag
     6061 gtctatcaca agaagctttt gctgaaaaag ctggattgca tagaacttat atttgtgcta
     6121 ttgaaagaaa aaagagaagt attgctttag ataatgtgca gaaaattgct gatgcgcttg
     6181 agattgagac ttacttacta tttatagaag gtgaggagaa agaatgtcta tgaaaaaatt
     6241 accaatagaa aagcttatag aaaatttagg tttttcaggg tcaaagaatc ttgtgaatat
     6301 agatatgctt acaaaattag atatatctaa acatgatgta aaaactttaa aagttataaa
     6361 tccttatgca atttatttag taaatagtaa gccatttata ttatttatag agtcttcttt
     6421 tgaagaaaaa aaattacaac aaattagtaa gcttatatgg aatgctcaaa taccaattgc
     6481 ttttatatgc gattctaatt ctgttaaaat atttaatgga aaagctttgg atttaaagtc
     6541 atatttaatt gaggaaatat ataatcaatc tattgaggat ttttcggaag aatctgagtt
     6601 ttcttatttg aaaatatctg atccattatt ttgggatcaa tacactaaac aatataccaa
     6661 aacgcattta aatgattatt tgctagataa tattacttca ttgacaaata aattaaagaa
     6721 aaaatataag gtgaaatttg caacgaagct tgttctaaga ttaatattta ttagatattt
     6781 aatcgataga ggtgtcgata tagcttataa aaattttaat catgatatta aagcttcaca
     6841 aaaagaactg ctaaagatta ctaaaaacaa agttgaatta tatgaactgt ttaaatactt
     6901 aaaagaaaag tttaatggta atttatttga gttaaatgac gaaattaata atacttgttt
     6961 aacagatgaa gtgtttattt tactatctga ttttttatca ggtaaaataa gtattgacaa
     7021 tggacaaatg tctttatttt caatgtatga ctttaatatc ataccagttg aactaattag
     7081 taatatatac gaaattcttt taggaaaaga aattcgtaat aaagataacg cattctatac
     7141 tccgaattac ttagcagaat atattttaga taaaacaaca cttgaatttc tgaaaaataa
     7201 taggcaatac aaaattctag atccttcttg tggatcagga gtatttttag taaatagttt
     7261 tagacgtatt attgatatga atttgggtga taaacttttt tgtgaagatg ataaactttt
     7321 aaaagaactt ttattatcta atatttatgg aattgatatc aatgaagatg ctatcgatgt
     7381 tactattttc tcgttatatc taactatttt agattacaaa gaccctaaga tattatcaga
     7441 atttaaactg cctaatttaa aagggtgtaa tttaatagcg agtgattttt ttgatgataa
     7501 taaattggag agtttaaaga aaattaactt tgattttatc attggaaatc cgccttgggg
     7561 aagtgtctca gatggattac atttagatta ttgcaataaa aatggctata atgatatgca
     7621 acaaaacaaa gaaatcagta gaagttttat ttttagagca aaagatttta gctctagaaa
     7681 tacagtatgt tcttttgtag tgcattctaa aatactatat actcaaaaaa agccatcaaa
     7741 aaaattccgt gaatttttgc ttaaaaaaag tcaaatattt agtgtagttg aaatgtcatc
     7801 tgtaagaaaa ttagtgttta aaaatgcaga cgctcctgca gcagttatat catttaagta
     7861 taacgaaagt gaagaaaatt taaataatag aatcatctat acatctttaa aaccaaatgt
     7921 attttttaaa ttatttaaca ttattgtaat tgaaaaatat gatgttaaat atatttcaca
     7981 aaaactatta ttggagaatg attgggcttg gaaaactatt gtgtatggat tcagtaaaga
     8041 tatagatata attatgaata tcaaaaaaaa atatcaaact gtaaatgaaa ttattttgaa
     8101 aaacagtttt gttcatggaa cagggataca aatcaatggt aatgaaagcc atgatgcgac
     8161 acatttaaaa ggaaagtggt atgttgattc aaagaaagga atagattcat tctatgttga
     8221 tataaataaa gggaaagaac ttactcaaga taaaatgaga agacctaagg ctaatcagca
     8281 agaaatattt attcctccat atgtattatt aaaaaaggga gttgacacga aaacatatag
     8341 atatagagca gcattttcaa atgatgcatt tgtatattca gatgcagtta caggaatatg
     8401 tggaaaaaaa gataatttaa ctacacttta ttgcttgact ggtatgctta actcttcttt
     8461 ttactcatac ttaaatataa tgctaggtac atcaattgga atagaaagag aacaaggatt
     8521 ttcaacggaa atttttacgt atccagcaat agaaagtgat gaaattgcta atctggtagt
     8581 aaaaatacaa gaagcgacta aattagaaaa aacaactatg ttcttgaatg aaaaaaatag
     8641 ttgtaagtat atcaaacaat tagataagtt aattttgaag tcttttaatt taaatgatga
     8701 taaatttatt gactatgcaa taaatattca aattcctgag cttacaaatt ctaagtcaac
     8761 taacatatat agaaaaataa ctaaggaaga tttaattaat tatagtgaat gtttcaaaga
     8821 acagttttca tcgatatatc gtagggttgg gaaaagtatt tcaatttcgg tatatccaaa
     8881 aatattaaat cgttttacag tatttgaact agaagtaaca gatgaaaaaa cagatagaga
     8941 gattatggtt aaagcagaag ctgataataa tatggagttg tttacgaaaa taattagata
     9001 tgattataat gatgtttttc atcaaattag agatattatt cattttggag aaaattcatt
     9061 ttatatcata aagcctaatt gctataaata ttggcatcct gctatcgcag aaatggatct
     9121 tgctgatgtt ttggatcaga ttttatctga tacagggggt gaagaataat gggaggattt
     9181 aaaaattctt cactaaactt ttatttaaag caagggcttt atgaaataat tatagatgga
     9241 ttaatagact gttgtttact tatgagcaaa tcatgtattg aacaatctag aaaaatatta
     9301 aatcacgaag aaaagataag aacgcattta gttgaaaatt atttagaaaa tgatattgaa
     9361 agaagaaaaa catctttatc taaacaacat ctaagatttg aagtagaagc acaagaaaat
     9421 tatgacaaca aaaatgaatc ttatgagggt agggttgata taaaagttat atctaaagac
     9481 actttagcgg atagaagggc ttattatatc attgaatgta aaaggataga tggtaaggaa
     9541 actttaaata aaaaatatat aaccgaaggt gtacaacgat ttattactga gaaatattct
     9601 tcgcattatg gcaaaaatat aatgcttgga tttatagtaa aagatataaa tatccagagc
     9661 aatatacaaa aaattaattc actgaatcag aacatattat atacatatat agatacagat
     9721 atgttgacag ttagaagtga ttctgaaaat aattatgctg tttgtgaaag taaatataaa
     9781 gtaaaatcta aactactatt gttaaatcat attttttata acttttcgag tataataaga
     9841 taatcttatg aatataaaat attactatgt taaaattata gtttggtttt gataatatta
     9901 taaaaataag tttaagaata aaatataaag gaaaaaaggg gcgtagcact attttttagt
     9961 gtgacagccc ctttaatttt aaaatcggta aggaggagtg tatttgaaaa aaattaagtc
    10021 atatacaggg attttgaatg tggagaaggt tatatatgca ataaatgatt ttcagcttcc
    10081 atttccagtt acttttacgc agatgacatg gtttgtatta acacttttta taattatatt
    10141 atttggtaag ttacctccat tatttatgat agaaggagca tttttaaagt atcttggcat
    10201 accaattttt ataacttagt ttatgtctca aaagactttt gatggaaaga aaccatatgg
    10261 atttttaaag tcaataatac tatattcatt aagacctaaa ataacatacg caggaaaaaa
    10321 cattaaattt aaaaataaaa ttattgatga aaatataact atagtaagga gtgaattata
    10381 tgttcccgat aaaatatata gagaacaatc ttgtatttaa taaggaagga gaatgttttg
    10441 catattatga attaatacca tataattatt cattcttata agctgaacaa aaatttattg
    10501 tacatgataa ttttaggcaa ttaatatcac aaagtagaaa tggtaaaatt catgcacttc
    10561 aaatagccac tgaaagttct ataagagtta cacaagaaag gttaaagaaa aatattacag
    10621 gaaaattaaa agaaattgct tatgaaaaaa tagacgagca aacagaggca cttgtttcta
    10681 tgattggcga taatcaaatt gattatcgtt tttttattgg gtttaagctt ttagttacag
    10741 agaaagaaat gaatataaag agtataaaaa agtcaatatt agctaatcta tcagatttta
    10801 tatatgatgt taataataaa ttgatggatg actttgtcag tataaataat gatgaaatta
    10861 tgagattttt aaaaatggaa aagttattag aaaataaaat ctcaagaaga tttaaagtta
    10921 gaaggttaga taaaaatgat tttggatatt taattgaaca taattatgga aagaaaggca
    10981 cagtttttga agattatcaa taccatttac ctacaaagaa acttaaaaga gaaacattag
    11041 taaagaaata caatcttata aagccaacta gatgtcttat agaagagaag caaagatact
    11101 taaaaattat acatgaggat tcagaaagtt ttgtagctta ttttactatt gaaattatta
    11161 ttggagaaat gaattttcca tctagtgaga tattctatta tcagcaacaa cagtttgcat
    11221 ttcctattga tactagtatg aatgtggaaa ttgtaacaaa taaaaaggct ttaactacag
    11281 ttagaaataa gaaaaaagaa ttaaaagatt tagacaatca tgcttggcag tcagggaatg
    11341 aaacagtaac aaatgtagta gaagcattag aggctgtaaa tgagcttgta aataatttaa
    11401 atcaaacaaa agaatcaatg tataagttaa gctatgtaat tagggtatca gcagatagca
    11461 ttgatgaatt aaaacgccgt tgtgatgaag ttaaagattt ttatgacgat ttaaatataa
    11521 aattagtacg cctaatggga gatatgatag gatttcacaa tgaatttata ccttcaagca
    11581 aaaggtatat aaatgattat attcaatatg taactagtga ttttctagca ggattagggt
    11641 ttggagcaac tcaatagctt ggagagaacg aaggaatata tattggatat agtttagata
    11701 caggaagaaa tgtctatctt caaccatctc ttgcaagtca aggagtaaaa gaatctgtaa
    11761 ctaatgcgtt ggtagcagca ttctttggat ttttaggagg tgggaaatca tttgctaata
    11821 acttacttgt ttactattca gtattgtttg gagggcaagc tgttttggtg gatccaaaag
    11881 ctgaacgtgg caaatggaaa gaaaatttac cagatatata tcatgaagta aatattgtta
    11941 atttaacaag tgatatagaa aataagggaa tgctagatcc ttatgtaatt atgaaagaag
    12001 taaaagatag tggaagtcta gctattgata tattaacttt tcttacagga ataagtagtc
    12061 atgatggaga gcagtttcct atacttagaa aatcaatcat aaatgttact caagacaaat
    12121 gcagaggact tttgaaggta atagatgaat taaggaaaga taattctcaa atatcaaata
    12181 atattgcaga tcatattgaa agttttacag attatgattt tgcacaatta cttttctcag
    12241 atggaaatat taaaggctca attaatctag aaaaacaaat gaatataata caagttgcag
    12301 atcttgtttt accgaataaa gatactttat ttgaagaata tacaacaatg gaaagtcttt
    12361 cagtagctat gttgatagta attagtactt ttgcattaga ttttatccat acagatagaa
    12421 gtacttttaa aatagtagat ttagatgaag cttggacatt tcttaatgta gcacaaggaa
    12481 aaactttatc taataaatta gttagagcag gatgtgctat gaatgcagga gtgtattttg
    12541 taactcaaaa tgcaggtgat gtatcagatg aaaatttaaa aaacaatatt ggattaaaat
    12601 ttgcattcag aagcacagat atcaatgaaa ttaagagaat acttgaattt tttggagttg
    12661 ataaggaaga tgagaataat caaaaaaggt taagagagtt agagaatgga cagtgtttaa
    12721 tacaagattt atatggacga gttggggtaa tacaaataca tctagttttt caagaactcc
    12781 ttactgcatt tgatacaaga ccacctataa gaaaagaggt ggattaatga aatcgaaagt
    12841 atttagaaat ttaagctgga taactatttt aatagtagta cttttactat tttttattta
    12901 tataaattta tagatttaga gaaacaaata gatacattag ataagaaaag aaaaaaactc
    12961 atagatatga gattagaaga tataattgat aaagaatcat atgaagaaaa gtatgatgat
    13021 ttaacagttg aaataaagaa acttagtaaa gagaaaaaaa ctcttaaagc atcattaata
    13081 aatgaaaaga gtataaaagc aagacttaga gaatttagaa agatacttga aagaaatgaa
    13141 ttgttagaaa aatttgatag atatgtattt gcaagtatag tagataaggt tatagttgga
    13201 gttattaatg aagatggtat agttgatcct tataaaataa cttttgttta taaaactagc
    13261 tttgatgata aacaagatgg acataaattt ataaataaga gtccgttaga aacgtattca
    13321 aaagataaca acgataataa aaagaagtgt tcatatacta ataatgagac attgcgagac
    13381 tgtagttaaa cttattagag aataacaagg tttcagactt ttatattgtg tcttaaaagt
    13441 agatttgcca cctatttgcc accataatta tttttgggtg gcaaatttat tctacaatga
    13501 tttcaaaaat atttaccgtc tggtttctca tgttgttagt tacatgtaaa tatgtatcca
    13561 tagttgttga agttttagca tgtccgagta tatgctggat atcttttata ttagtaccat
    13621 tttctaataa catagtatca gggtttttaa aataaagtgt gtattcttct aaagtagtga
    13681 taattaaaat atcaaatatc actatacaaa ggaaagtgca cgctttttat gtttaacaaa
    13741 aacgaaatat taaaaaactt attgaagaat atgatgtcaa aacaactaca gatattcaag
    13801 atatgctaaa agatttattt acaaatacaa ttcatcaaat atttacattg tcgaatttta
    13861 gcggtttaaa cataatatat tgactgatat aataaaatca tagaatattt tagggggaaa
    13921 tagattatgt ttaagaaaaa gttaatagct gttgcaatga gtataactat gattagtgta
    13981 gggagctttt cgtatgctca ttcaggaaga actgattcat caggaggtca tcgtgataat
    14041 aaaaataaat ctggattagg aagttatcac tatcattgtg gtggttatcc agctcattta
    14101 cataataatg gtggttgtcc ttatactgga ggaggaagca gttctggaac aacaacttcg
    14161 gttaataatg aagaaaagca aaagaggtct gttggagaaa aaggatataa tcaagggtat
    14221 gaagatgggt ataaaggaaa ttatagttca tcaaattata gtggagatta tagtgataca
    14281 tatgaatcaa agtactcaga aggatatgaa aaaggtaagg ctaaattaga agaggaagaa
    14341 aaggtggcta aagagactgg atataattta gggcttacag gtgctaaatc taataataca
    14401 tatgaaaaag aagttttaaa aaatgcttat gataccggat attcaactgg atacaatgaa
    14461 tataagacta aaaagataga agaatataaa gctaaaggga tagaggatag caataaagat
    14521 aaggaaaaaa tgacatttga agaaaacata gattctgaat ttatagatgc ttataataat
    14581 gcttatgacg aaatacaaga acaattaaaa aatgattaca ctactcaagg atttgagagt
    14641 gctatcaaag gagaaagatt tgacacatca actataggta atgttaaata tgctaattgg
    14701 tttaaagaag gatatgataa tggtaaggtt aagttaccta aagcaaaaga aagtgtatat
    14761 aatcaaggtt acaatgaaga agattttagt ataccagatg aaatgaagtc tattgaaact
    14821 aagttaaaag gtgtatatga tgaaggttta gaaaaaagag aagaagagaa aagtagaaat
    14881 gctacttatg gggttggagc tggaacagca gctgtagttg ctggagtagt atataagaag
    14941 aaaaaacgta aaaagatata gatgctagat ataccaatga tgacaagtgt ataacaaata
    15001 taattttaag tataaatatt taaaatatag aagggaaggt tatatctatg ggatttaact
    15061 ttaggaaaag cataaatcta ggaggaggac tcaaattaaa tttaggtaag aagagtgtag
    15121 gtataagtgc tggtgttaaa ggtgctagag tgtcagttaa ttcaaaagga agaaaatcag
    15181 ctacattgag tatacctgga actggaatat catatacaaa aactagtaca aagaaacgta
    15241 aacctaaata aatatttgat atactaagaa atagactgtt acaaatagtc tattttttag
    15301 tatatagatt taaaaagtga caaaattttg aaatataaac taatttcata aaggaaaata
    15361 ttattttatg tagaattata atatttaagt aatattataa aatttatagg aggatttcat
    15421 tatgaaatta agcaaaatac tattggcaat agggatgtct gtttcattat tgattgctag
    15481 ttgtccaaca tcaaacgctc ttagttctat taattcaata aaaggtacaa ataagtatga
    15541 aacagcaggt ttaatagcag ataaacaaaa ttatgatatt gcaatactta ttaattctga
    15601 aaatggatta tctgatggat tgtcagcaag tggattgtca ggagctaaaa atgccccaat
    15661 attgttaact aagaaagata caatcccttc agaaacatta aaaagattag aaaaggtaaa
    15721 agaagtatat ctaattggag gaaaaaattc gataggagca tcagttcaga aaatattaag
    15781 tgataaaaaa ataagcataa atagaataag tggaactgat agaatagata caagtcataa
    15841 agttgcaaaa gaaataggaa gagtaagtac ggttaaaaaa gtaatattaa ctaatgcttt
    15901 taaaggcgaa ccagatgcta tgagtgttgc ttctatagca gttagagata aatctccaat
    15961 agtattaact gatggaaata ctacaagttt tgctacagat ggattagaaa cttatgcaat
    16021 tggtggaaaa gtcagtatga gtgataagtt aataaatgaa actaaagcaa caagaatagg
    16081 tggagtagat agatacgata caaataaaca aataataaat aaattttata atggctcaaa
    16141 agaattttac atagcaagtg ggactgattt agtttattct ttagttggtt ctacaattgc
    16201 acaaaaaaca cctatagtgt tagttggaaa tggaagtaat aagagtatat taaaaaatgc
    16261 aagtaaaata actgctattg gaaatattga tgatgttgtt ataaaacaat gtttaaatat
    16321 aactaataat gttggggatt caaatacagg tattgtaaaa gatactaatt caaataatac
    16381 gaacaaacca agtaatgagc ctagctctaa taatcctgga ttaactgata aaagtacagt
    16441 atatgtaact cctaatggaa aatcatatca tctaacaaaa aattgttcta cactaaaaag
    16501 aagtaaaaca ataaataaag taacattaaa acaagctaag tcacaaggta aaatagatcc
    16561 ttgtaatgtt tgtgtaaaat aatgtattag ccaaatatat attaaaaaag acaattaatt
    16621 aagattgtct tttttattga agaaatataa atttatatta tgtattatta aaaaaaaatt
    16681 attaattata aaaaataata ataaagacga taagtattaa aattttatgt ttccaatatt
    16741 tatgatataa ttaatatggc aaggaataaa tatttgaaaa agctatgagt ggtgtttcca
    16801 tggaagtttc ttcactttct tatgagagga ggtgaaaact atggaaaatc taatgatgag
    16861 tattataact ggtgttatag ccagttacat ttacgataaa ttaaagagcc actctgagcg
    16921 accaactaag agtggctggg aacttaatat aaagttccag aaaagtaaat cttcaaaata
    16981 atttgttgaa ggaaacatca ctcaaagtta agtaaaatat gtttccttgc ttttattata
    17041 tcacaaatta aaaatattaa cacaaaattt tgtgaatata tacttattat tgttctaaaa
    17101 aaatatgtaa ataattgatt tgattgcatg gaattattga tgtaatattt aagttaaata
    17161 tacacaatct caattaaaga agataagtac tataaattca tatcaaaaaa atacataaac
    17221 ttttaagtta taattttata aaaagtcttt tagtatcatt atttcaagag gaattatcat
    17281 atgaaaaaat aacttcatta gaaaagtgga taaaaaactg gtaagaagat gactaaagat
    17341 tacatatact attacttaaa ttaaaaattt aatataattt ataatgtata c
//
LOCUS       DAMJYR010000080        17291 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.080, whole genome shotgun sequence.
ACCESSION   DAMJYR010000080 DAMJYR010000000
VERSION     DAMJYR010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 17291)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17291
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.080"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            27..773
                     /locus_tag="PC471_002535"
     CDS             27..773
                     /locus_tag="PC471_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HDF2789654.1"
                     /translation="MKIENLSIDDIKRGYIFNDDLNCYSCIKCTKTYEVGEIYKIGNR
                     FFEASKAIELHMELEHSDYLEQLLNSDSKYNTLTENQKQLFQLFISDLPDKEIAKQVG
                     VSTSTIRHQKFIFREKAKQAKLYLALYESVFEDKSNKSSTIIPIHEHARMVDERYVVT
                     EEERKHILDTSFESMNPLKLKVFSSKEKKKVVILSKILEQFERGKKYSEKEVNQILES
                     IYDDFATIRRYLIEYGFMSRNKECTKYWLT"
     gene            834..1508
                     /locus_tag="PC471_002536"
     CDS             834..1508
                     /locus_tag="PC471_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase"
                     /protein_id="HDF2789655.1"
                     /translation="MNDELKSILYNKGVDIVRFVDISEFPINQTQGFSKAILFCIGLS
                     KKFITDIYNNLPTDSDEFLEKEEKAEELADWISEYIQNKGYRAYSQSEKNNLEHGYFE
                     KAYINPEMQSGISPLPHKTIANISGIGFMGKNNLFVTEEYGCAFSMCTVLTDAPISVE
                     RYPLIDSKCMDCNVCVENCPAKAIHGNEWTLPGKRESIIDVSKCFCVLKCMMSCPWSL
                     RYANQK"
     gene            1876..3222
                     /locus_tag="PC471_002537"
     CDS             1876..3222
                     /locus_tag="PC471_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 55 protein"
                     /protein_id="HDF2789656.1"
                     /translation="MEIETKTPDWINVLELNSKIDITGKYDVSNIIQDIVSDKSLEGS
                     IIYFPKGNYLFEKGIKISQQITLQGDSYYGGDNQVSNLDKKQSIIGTTNFITRGVSNM
                     SIIALTGTSQCIKNINFYYDSHDIEKIPPKNVSAITEYGETQGLSHFEHLFISGFSGI
                     GIEIPYYSTGNDIIVSSCGLGMRLGEKSMLSSSKIYECKNGMEITTGVSLNNVRIEEI
                     KEIGINNKGFGFNLIMNLMVDQCGYCGFMFEKMCYSQITARITRCGQYYKSVDYNAYE
                     NMEDRKEKAYSIFYGDILESCNIVLMNRNIDTLESDSLNEHKIYVIKANETNNVMLTC
                     YAEADNFIQCAKGNLLLENGENTYKFYNGEICSVGGVQISDIDEKDLIKIIENTLYID
                     DKGKYLMVPRLKKNTVIYSVLDSTEEINKLHGGIWEQIDVKVLAGESMYYYKKIKD"
     gene            3249..4649
                     /locus_tag="PC471_002538"
     CDS             3249..4649
                     /locus_tag="PC471_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 55 protein"
                     /protein_id="HDF2789657.1"
                     /translation="MEESIIIKDISGWINVLDYGICNDETKDVSFKLQEIMDAVPEDS
                     IIYFPKGKYLFESGVKIKKRLTLCGDSYILANVPKNTAGVTQFTFKGKKNPKSENRAI
                     ITAKSVRHCIKNICFYSDSCDRDMTSNHEPPTDGKPRFHHNVIINYRNISAVICEDKQ
                     GEPGHYENLSFSGFSGIALDMPNNSTANDITIFTSGLGISTGENSTIANSKVWGCVDG
                     MIISTGTFINGMRVEEIKKVGIKNIGRGLNFITNITIDQCGYCGVWFDSISNVQISGN
                     ITRCGQYYYDVSYDNYLMLDNRVKEAYSLFYGNLLESSNIVLSNTNEDNWEDFKEDKH
                     KVYLIEALKTKNVLLTCNIDATDWIVKSEKGNLTYNNMRQTVIFYDGKLSSINGIGIS
                     DEDYGDKVKIKKGNIYINSNDKDIKMHKPEVGDIMSTIIKDKSILSAHYGGTWEKIGE
                     KIEFGITVHYYKLIEK"
     gene            4859..6295
                     /locus_tag="PC471_002539"
     CDS             4859..6295
                     /locus_tag="PC471_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 55 protein"
                     /protein_id="HDF2789658.1"
                     /translation="MEGRTGIKDGRWINVVERGIKNDGSYNIGPALQDIIDEIETDFC
                     VLYFPKGKYLFTSGVKINKEIVLQGDSNSSTGTTQFITRGVENMSIITLTGKKQCIKN
                     INFYSDSCEIQVNKEPPTKGNPKYHYEMIINKNEKNVEMNNVSAILYDNSKNMIKGLG
                     HYENIYISGFSGTGIRIPYYSIVNDITVSSCGLGIDTGIDTIISNSRISKCQNGMRIR
                     TGTSINNVRIEKIQKVGLISSQVKGYEGYGSYKINNITIDQCGYCGFSFDSMKDSYFS
                     GIIRRCGQYYYNTDYDTYLSIKDRVEEAYSLFYGNYFTECNIDLVSDNKDNWDDGLEY
                     KHKVYVFKTLQIPKLTLRCNIDAYDYIVKSVHGGGNLMLKNNFNNYIFIDSSNPEVIN
                     GINFNYQKNGNLLRLNNGKLSYKNGKEETAIFRPNYGLIVASTDSECEQIEATYGEKW
                     EKLGSEVIGEETVYYYKHHEPDKTNQIQ"
     gene            6639..7583
                     /locus_tag="PC471_002540"
     CDS             6639..7583
                     /locus_tag="PC471_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1848 domain-containing protein"
                     /protein_id="HDF2789659.1"
                     /translation="MIVSVSRRTDIPAFYSKWFFNRLEEGFVYVINPMNPKQVSKIEL
                     NPHTVDCFVFWTKDVTPMLYDLNKLRDYKYYFHYTITSYGKEVETCILDKRKVIDSFK
                     ELSRKIGKERVILRYDPIFLSEKYTVDYHIKAFASLCNQLDGFTEKCIISFIDLYKKT
                     KFNTKALHIKPIEITEIETLSKEISKIAHKHGIILEMCSEEYNLSKFGIGKSKCIDDR
                     LISKIIGCEVEVKKDSTQRDICGCVKSVDIGQYNTCRHYCLYCYANFNYAKVEENCRL
                     YKEDNKLLIDTLRDDAKISIRDMKTIKVDKNKQISIFD"
     gene            7752..8684
                     /locus_tag="PC471_002541"
     CDS             7752..8684
                     /locus_tag="PC471_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HDF2789660.1"
                     /translation="MNLLKKYPIPIASLILSIFTLGNLLQSYSESLRNVIGFIGFILY
                     AIYIVKIILLRKGVKEQLENPLISSTFPTITMATMIFATYIKPLSLELSIMIWSIGIV
                     GHLVLIVLFSKKFLVKFSIKTVFPSWYIVYVGIVAASVTAPAFNQLLIGKIAFWIGFV
                     SYIVLIPVILKRVWIIKEMPEPSLPSIAILAAPGSLLLSGYINSFSDKNNVVLYLLFI
                     LSIVFYIIVLGYLPKLLRLPFYPSFAAFTFPMAISAMGMKLMNGYLTKSGHFISWISY
                     ASKLEEIIATVIILYVLVLFIIYLSKSETTSKAK"
     gene            8789..9256
                     /locus_tag="PC471_002542"
     CDS             8789..9256
                     /locus_tag="PC471_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C_GCAxxG_C_C family protein"
                     /protein_id="HDF2789661.1"
                     /translation="MKTKEEYLKEVRNISEEYFRKGEFFCSEAVLQTINDALGQPLSP
                     EITKLASGFPIGLGKAQCLCGAISGGEMALGIVYGRVHGEAMNPKMFEHAKGLHDYIK
                     KEYGATCCRAITKKWDGDNFMSPERKEHCIKITGQVTEWVADKLIEDEKLNIK"
     gene            complement(9500..10354)
                     /locus_tag="PC471_002543"
     CDS             complement(9500..10354)
                     /locus_tag="PC471_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009912760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HDF2789662.1"
                     /translation="MLTIIIALLPVMGWGLMPIVANLKKSSPHEQLLGTSISAFIFST
                     IITLVIHPEITFFSFCISMISGIFWSLGQLMQFKAIQIASVSKVMPISNGSQLTFTTL
                     LAVILFNEWTSSKMLLIGSLSILCIILGILLTNYQTKKSTDKNMLKCVGVVLLSSLFL
                     SLYVVTNQIFLIEGYRIILPQSVGMLITSSIIVKYFSKEIVYRENVLFNLITGILWSI
                     ANLGMFITTNTLGVTISFSISQSCVIVATLGGIIFFKEKKNKKEWLAIFIGIILIMSG
                     VFMLSIIK"
     gene            10520..12484
                     /locus_tag="PC471_002544"
     CDS             10520..12484
                     /locus_tag="PC471_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HDF2789663.1"
                     /translation="MLTDMIQNREIKIIKFLLKEGHTTVKAIADDLDVSEKTVSKSLK
                     EVEAVLKKIGLSLIRKPKIGVYIDGDLKKILPYLDNKGSQIPTTREERVIYIYSVLLK
                     SNDYITIQQLSDELYISRGTIERDLAEVEKLLKNEGISIYKKPSKGIKLNISERDKRQ
                     LTAKFIHKFWSRNWYIKQEQESFYQAFEKIQADVNGIFSEDDLSIIIDILRNFSRERK
                     FEFSDYEFQSLVIHLAIAVERIRKGEYIEESLYTGNIENNQMSNTNYLAKQLEEKFSI
                     LLPKSEIGYINLHLVASDQHRDTNELKNTSTNPCETSTNNISNLREIIKNTLYETAYD
                     KDLIDGLTSHVHSSINRLKYGLSIKNPYVNTIKQNFSLSFEQALDLKKVIEFIYDIRI
                     NDDECAYIALHFEAFRERMKELPQNIRVILVCSTGLGSSRLLSARIKKYFPMIKIEKV
                     LSVQELVSSKIDADLVISTIYLELETIPTIVVSPILNESDIRTLERNIQNVSSYTSKK
                     YKSFRNLIFEENILLNIEASNYEDAIKYITDNLIKKGFAKEGIAKSAIERENLSYTSF
                     QSIATPHANPSYIKKSNISVATLKNPITWGNEKVEVIFFISLENDKNLELDEIYEVFY
                     DFIDNKKNINDILSATSTKEVYDLLVEESI"
     gene            12484..12945
                     /locus_tag="PC471_002545"
     CDS             12484..12945
                     /locus_tag="PC471_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDF2789664.1"
                     /translation="MKISDVLKKEQIVFNLDTNTKEETIKKLSSIFVENGIVDNEEEY
                     VKSVLEREEHSTTGIGNGIAIPHGKSNAVKKSSIIFAKTKHKIEWDALDEKPVDSIFL
                     LAISNIDKDSNHLVLLSKLATKLMDDDNVDALKLANSEQEIINIFSEEGEM"
     gene            12948..13268
                     /locus_tag="PC471_002546"
     CDS             12948..13268
                     /locus_tag="PC471_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HDF2789665.1"
                     /translation="MKRKIIAVTACATGVAHTYMAAQALKKASKKMGHLIKVETQGAT
                     GIDNELTTKDAQIAEVVIFAVDTKVRNAERFEGKEILEVPVNAPIKDAEKVINDALKL
                     IDTK"
     gene            13284..14384
                     /locus_tag="PC471_002547"
     CDS             13284..14384
                     /locus_tag="PC471_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HDF2789666.1"
                     /translation="MLKKIVSELKKHVLTGISYMIPLVIAGAMIMAISRVGGSFYNIP
                     DIWDAKYAESASSIVRLLHDLDGFGGTALGLMFPVIAAFIGFSIADKLAIVPGLVGGM
                     IAKDIGAGFLGALAVGLIAGYTCLFIKNHVKLPKSAASIVPIFIVPVFGTLITVVLIN
                     YVIGIPFATLNTGLENWLNGLSGTNQILMAAIIGAMMAVDLGGPVNKAALTTSLALLT
                     SGIYAPNTAAMVGIVIPPLGLGLATILAKQKYNKQLREAGKSSLIMGLIGVSEGAIPF
                     AVESPLKVIPSCVIGTAIASAMAVGLGSVNATPISGFYGWFTVENWPMYVLSIAVGTV
                     IVAGLVIVLRGNPVIEDDEFEDDDFDEAEWES"
     gene            14504..17104
                     /locus_tag="PC471_002548"
     CDS             14504..17104
                     /locus_tag="PC471_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HDF2789667.1"
                     /translation="MIKAHIVNHTHWDREWYFTSGDALVLSEQLFTDVIDELERNPDV
                     SFVLDGQLSILDDYVQLHKEKIEIIKKLIKEDRLHIGPWFTQTDAFFARGESILRNLM
                     IGIFESKKYGKYMKIGYLPDTFGFNAQMPILLKHVGLDNIFFWRGIHLGKQVQSPYFK
                     WKSLNGESYIYAVNMPHGYGTGMLLEPSLKYVNGRLDPAIDFIKSYTDVDEVLIPSGN
                     DQLNIIGDFKNKIKAINDIGKYSYITSSYQEFLKYIKSIESLESYRGEFREPVLARIH
                     KSIGSSRMDIKLACDKLENKLIKRIEPLLVIAKKSKIEISNQLLINTWKKLLEGQAHD
                     SLAGCVTDTVTDDILHRIREANEICDSIENTIVKKISEGLKLSKNDILVFNTEAKRFN
                     GYKEIQVVSDSKNIYFKDNLDATIIEETYVKPRENVLEETPAGNIFIEEPGYYILKVR
                     INVQLPALGYKVVSFESSDKEMVSLNKSNDAFISNDNYKIIYEDGSLNLQLKDGTYIS
                     NFLTLKDSGNAGDTYDFSPLKNDEDIKLSFDKVYTEKALGYEKMVVKGTTLLPLTLED
                     RKNKYLNGKLNVKVSIVLSKENPLMDVKLCVDNTIYNHRLRVHIKTDIKDNKNIASLP
                     FGYITRENGVLDNWEDIYSEMPIDLEPLESNITLTNQNRSCTIFTKGIKEYQHIEDEI
                     ALTLLATTDELGKPDLLYRPGRASGDTTKKGHIRIKTDKAQMLKKLEFSFAIYMDSKS
                     FDELEIANLTHNYLRECVNYQLQDYNFFLYRIDNKIQKSIVNKTVSRELEIISLPENY
                     LISACYPSYYSKDKFIIRVENPSSNKMILDEQIFKNRNGKIVNAIEDVEEEQVYEIAP
                     FDVISILLDL"
BASE COUNT         6868 a         1783 c         3001 g         5639 t
ORIGIN      
        1 gtaaactgtg aaatggaggt agtaacatga aaattgaaaa tctttctatt gatgatatta
       61 aacgaggcta tatatttaat gatgatttaa attgttatag ttgcattaaa tgtacaaaaa
      121 catatgaggt aggagaaata tataaaatag gtaatagatt ctttgaagct tcaaaagcta
      181 ttgaacttca tatggaatta gaacatagtg attatttaga gcaattgctt aatagtgatt
      241 caaaatacaa cacattgact gaaaatcaaa aacaattatt tcaattattt attagtgatt
      301 taccagataa ggagatagca aaacaggttg gagtatccac atctactata cgccatcaaa
      361 aatttatttt cagagaaaaa gcaaaacagg ctaagctcta tttagcacta tatgaatctg
      421 tatttgaaga taaatctaat aaaagtagta ctataatacc aatccatgaa catgctcgaa
      481 tggtagatga aagatatgta gtaactgaag aagagagaaa acacattttg gatacatcat
      541 ttgaaagtat gaatccttta aaattaaaag tattttcttc aaaagaaaag aaaaaggtcg
      601 taattttatc taagattttg gaacaatttg aacgtgggaa gaaatattca gaaaaggaag
      661 ttaatcagat tttagaatct atatatgatg attttgcaac aattagaaga tatctaattg
      721 agtatggttt tatgagtaga aataaagaat gtacaaaata ttggttaaca taattaaaat
      781 aataaatttt actaatgtgt aatgttattt ttatctaggg ggaggagtca aatatgaatg
      841 atgaattaaa atctatttta tacaataaag gtgtggacat tgttcgcttt gttgatatat
      901 ctgaatttcc aataaatcaa acgcaaggtt tttctaaagc aattttattt tgtatagggt
      961 tatcaaaaaa atttattaca gatatataca ataacttacc tacagatagt gatgagtttt
     1021 tagaaaaaga ggagaaagca gaagaattag ctgactggat ttcagaatat attcaaaata
     1081 aaggatatag agcgtattct caatcggaga aaaacaatct agaacatgga tattttgaaa
     1141 aagcctatat taatccagaa atgcaatcag ggattagtcc tttaccacat aagacaatag
     1201 caaatattag tggcattgga tttatgggaa aaaataattt gtttgtaaca gaggaatacg
     1261 gatgtgcttt tagcatgtgt acagtactta cagatgctcc aatttctgtt gaaagatatc
     1321 ctttgattga ttcaaaatgt atggattgta atgtttgtgt agaaaactgc cctgcaaaag
     1381 ctatacatgg aaatgagtgg actttgccag gaaaaagaga gtctattatt gatgtttcaa
     1441 agtgtttttg tgtattgaag tgtatgatga gttgcccatg gtcattaaga tatgcaaatc
     1501 aaaaataatt ttactagaga aggtattaaa ttgaatatta taaaattttt aaaatgtaag
     1561 attatataag atttttcaaa ataaaaattt gtatagaaca ctcagaaatg agtgtttttt
     1621 tattggaatt aaattatatt gatacaaagt ttaactattt taatcattat ataaaatttg
     1681 tattaaaaaa ttaagaataa cttggtaata gtgaaaatat aatgagatta taattgattt
     1741 ataatataac tataatgatg ttgtaaaatg aaaattgtca tttcaaaatt cccaaaaaga
     1801 gtaaaagtca cttcggttat agtcaaaaaa ttttttcagt gaaaagaatt tagatttaaa
     1861 agggaggaat taatcatgga aattgaaact aaaactccgg attggattaa tgtactagaa
     1921 ttaaacagta aaatagatat tactgggaaa tatgatgtaa gtaatattat acaagatatt
     1981 gtctctgata agtcactaga aggttccatt atatattttc caaaaggtaa ttatttattt
     2041 gagaaaggta ttaaaattag tcaacaaata acacttcaag gagattctta ttatggagga
     2101 gataatcaag tatcaaattt agataaaaaa caatcaatca taggaacaac caattttata
     2161 actcgaggag tttctaatat gagtattatt gcattgacag gaacaagtca atgtatcaaa
     2221 aatattaatt tttattatga tagtcatgat atagagaaaa taccgcctaa aaatgtatct
     2281 gctattacag aatatggaga aacacaagga ctatctcatt ttgaacacct gtttatttct
     2341 ggattttcag ggatagggat tgaaattcca tactattcaa ctggcaatga tataattgtt
     2401 agttcatgtg gtcttggtat gaggcttgga gagaagtcaa tgctttctag tagcaagatt
     2461 tacgaatgta aaaatggaat ggagattact actggtgtta gcttaaataa tgtgagaata
     2521 gaagaaataa aagaaatagg tattaataat aaaggatttg gttttaacct gattatgaat
     2581 cttatggtcg accaatgtgg atattgtgga tttatgtttg aaaaaatgtg ctatagccag
     2641 ataactgcca gaattactag atgcggtcag tattataaaa gtgttgatta taatgcttat
     2701 gagaatatgg aagatagaaa agaaaaagct tattccattt tttatggaga tattttagaa
     2761 agttgcaata ttgtattgat gaatagaaat attgatacct tagaaagtga ttcacttaat
     2821 gaacataaga tatatgtaat caaggcaaat gaaacaaaca atgtaatgct aacatgttat
     2881 gctgaagcag ataactttat acaatgtgca aagggcaact tgctgttaga aaatggtgaa
     2941 aatacttata aattttataa tggtgagatt tgttctgtag gaggtgttca aatttctgat
     3001 atagatgaaa aagatttaat caaaataata gagaacactt tgtatataga tgataaaggt
     3061 aagtatttaa tggttcctag attaaaaaag aatactgtaa tatattcagt actagattct
     3121 acagaagaaa taaataaatt acatggtgga atatgggaac agattgatgt aaaagtatta
     3181 gctggggaat ccatgtacta ttataaaaag ataaaagatt aaattatcaa actaggaggt
     3241 agtgattaat ggaagagtct ataataatta aagatataag tggctggatt aatgtacttg
     3301 attatggaat ttgtaatgat gaaacaaaag atgtaagttt taagttgcaa gagattatgg
     3361 atgctgtacc agaagattct attatatatt ttccaaaagg aaaatactta tttgaaagtg
     3421 gtgttaagat taagaaacga ttaaccttat gtggagattc ttatatttta gcgaatgttc
     3481 ctaaaaatac tgctggagta acacagttta cttttaaagg aaagaaaaat cctaaaagtg
     3541 aaaatagagc aatcataaca gcaaaaagtg taagacactg tattaagaac atttgctttt
     3601 attcagatag ttgtgataga gatatgacat ctaatcatga acctccaaca gatggaaaac
     3661 ctagatttca tcataatgta attataaatt atagaaatat atctgctgtt atatgtgaag
     3721 ataaacaggg agaaccaggt cattatgaaa atctatcttt tagtggtttt tctggaattg
     3781 cacttgatat gcctaataat tctacagcaa atgatattac aatttttaca tccggacttg
     3841 gaattagtac aggggaaaat tcaacaattg ctaacagtaa ggtttggggc tgtgttgatg
     3901 gaatgataat ttctactgga acttttataa atggtatgag ggtagaagaa ataaaaaaag
     3961 ttggaattaa aaatattggt agaggtttaa attttattac aaatatcaca attgaccaat
     4021 gtggatattg tggagtttgg tttgattcta tatccaatgt tcagatatca ggaaatatca
     4081 caaggtgtgg gcagtactat tatgatgtaa gctatgataa ttatttaatg ttggataata
     4141 gagttaaaga agcatattct ttattctatg gtaatttatt ggagtcaagt aatatagttt
     4201 tatcaaatac caatgaagat aattgggaag atttcaaaga agataagcat aaggtgtatc
     4261 ttatagaagc acttaaaacc aagaatgtac tattaacatg caatatagat gctactgact
     4321 ggattgttaa gtcagaaaaa ggaaatttaa cgtacaataa tatgcgacaa actgttatat
     4381 tttacgatgg aaaactaagt agtatcaatg gaattggtat atctgatgaa gattatgggg
     4441 ataaagtcaa gataaaaaaa ggaaatatat atataaatag taatgataag gatattaaga
     4501 tgcataaacc agaggtaggt gacattatgt caacaattat taaagataaa agtattcttt
     4561 ctgctcatta tgggggaaca tgggagaaaa ttggtgaaaa aatagagttt ggaataactg
     4621 tacactatta taaattgata gagaaataag taaatacgta gtatttgaat agatgtattt
     4681 tattggagag ttaggactat agttttaata taaatggttt ggtaatagca tggattgaat
     4741 aatataaaaa taatataaaa aattaatttg attatgtaaa tttctatgaa gtagtaaaac
     4801 tgtgacttta gtcataggag gttcaaacta agtaaaaggg aaaggaatat ataatattat
     4861 ggaaggaaga actgggatta aagatggaag atggattaat gtagtagaaa gaggtattaa
     4921 aaatgatggt tcgtacaaca taggtcctgc tttgcaggat attattgatg aaatagaaac
     4981 agatttttgt gtcttatatt ttccaaaagg taaataccta tttacaagtg gtgttaaaat
     5041 taataaagaa attgtattgc aaggtgattc taattcatct acaggtacta cacaatttat
     5101 aactcgtgga gtagaaaata tgagtattat tacattgaca gggaagaaac aatgtattaa
     5161 aaatataaat ttctattctg atagttgtga gatacaggta aataaagaac caccgacaaa
     5221 aggtaatcct aaatatcatt atgagatgat tattaataag aatgaaaaga atgttgagat
     5281 gaataatgta tcagcaatcc tgtatgataa cagtaagaat atgattaaag gtctaggtca
     5341 ttatgaaaat atttatataa gtggtttttc gggtacagga attaggatac catattactc
     5401 aattgtaaat gatattacag tttcatcttg tgggctgggt attgatacag gaatagacac
     5461 aattatttct aatagtagaa tatctaagtg tcaaaatgga atgaggatta gaactggaac
     5521 tagtataaat aatgtaagga ttgaaaaaat acagaaagtt ggtcttataa gtagtcaagt
     5581 taaggggtat gaggggtatg gaagttataa aattaataat attacgattg accaatgtgg
     5641 gtattgtggc ttttcatttg attcaatgaa agatagttat ttttctggta ttataagaag
     5701 gtgtggtcag tattattata atacagatta tgacacatat ttaagtatta aagatagagt
     5761 agaagaagca tattctttat tttatggaaa ttattttaca gaatgtaata ttgatttagt
     5821 tagtgataat aaggataact gggatgatgg cttagaatat aaacataaag tttatgtatt
     5881 taaaaccctt caaataccaa aattaacatt gagatgcaat atagatgcct atgattatat
     5941 cgttaaatcg gtacatggag gaggtaattt aatgttaaaa aataacttta acaattatat
     6001 atttattgat tcatctaatc cagaagttat taatggaatt aattttaatt atcaaaagaa
     6061 tggaaacttg ttaaggttaa ataatggtaa attaagttat aaaaatggaa aggaagaaac
     6121 agctatattc agacctaatt atggtcttat tgtagcatca acagattcag aatgtgaaca
     6181 aatagaggct acctatggag aaaaatggga gaagcttggt tctgaagtaa ttggagaaga
     6241 aacggtatat tattataaac atcatgaacc agataaaaca aaccaaatac aataaaattt
     6301 gtattatata atgatataaa gttttaatat atagtgttta cctttaatgt aatattggta
     6361 tatttgtata gtattaaatt tgttaaaaag taaaattgta gggtgcttga aaatagcacc
     6421 ttttttagtt tcattataaa attagttttg aaagtttagg aataatgtat atttattttt
     6481 aaagaattat aaccttatta aggatatttt aaatgctaga aatgaatcag taaatcatac
     6541 tggaattatc ttaaatgttt tgattaaatt aagtatttag agctataatt atattaaaaa
     6601 tatattagaa cataattata ttactatgga ggttaaatat gatagtaagt gtgagtagaa
     6661 gaactgatat acctgctttc tatagtaagt ggttttttaa cagacttgaa gaaggttttg
     6721 tttatgtaat taatccaatg aatccaaaac aggttagtaa gatagagtta aatccacata
     6781 ctgttgattg ttttgtattt tggacaaagg atgtaactcc aatgttatat gatttaaata
     6841 aattaagaga ctataaatat tattttcact atactataac atcatatgga aaagaagtag
     6901 agacatgtat tttagataaa agaaaggtaa tagattcatt taaggaattg tctagaaaaa
     6961 taggcaaaga aagagtaatt ttaagatacg acccgatttt cttaagtgaa aagtacacag
     7021 tagattatca tatcaaagca tttgcgagtt tatgtaatca gcttgatgga tttacagaaa
     7081 aatgtataat aagttttatc gatttgtata agaaaacaaa atttaatact aaagcattac
     7141 atataaagcc aatagaaatt acagaaatag aaacattatc aaaagagatt tctaagattg
     7201 ctcataagca tgggataata cttgaaatgt gttctgaaga atataattta agtaaatttg
     7261 ggattggaaa aagtaagtgt attgatgata gattaatatc aaaaattata ggatgtgaag
     7321 tagaggtaaa aaaagatagt acacaaagag atatttgtgg atgtgttaaa agtgtcgata
     7381 taggacaata caatacttgc agacattatt gtctgtattg ttatgctaac tttaattatg
     7441 caaaagtaga agaaaactgt aggctttata aggaagataa taaattactc atagatactt
     7501 taagagatga tgcaaaaata agcattagag atatgaagac tattaaagta gataagaata
     7561 aacaaattag tatatttgat taaaataaat atactaatta ccttaatata ttaagaatat
     7621 agatactttt gataacaaaa ttaaacttaa tagatatttt ctatataaga aagatggtca
     7681 taatatgtca atttgaccat ctttttgata taattaaaac acaaataatt tagtaacaaa
     7741 ggaggcagaa cttgaattta ttaaaaaaat atcctatacc tattgcaagt ttgattttat
     7801 caatttttac tttaggtaac ttattacaat cttatagtga atcattacgt aatgtaattg
     7861 gatttatagg atttattttg tatgcaattt atatagtcaa aataatatta cttagaaagg
     7921 gtgttaaaga gcaattagag aaccctttaa tatcaagtac atttccaact ataacaatgg
     7981 caacaatgat atttgctaca tatataaaac cattatcttt agaattaagt attatgattt
     8041 ggagcatagg aattgtgggt catcttgtgt taatagtctt atttagtaag aagttcttag
     8101 ttaaattctc aataaaaacg gtatttccat cttggtatat agtatatgtt gggatagttg
     8161 cagctagtgt tacggcacca gcatttaatc aacttttgat tggcaaaatt gccttttgga
     8221 taggatttgt atcttatata gttcttatac cagtaatttt aaagagagta tggataatca
     8281 aggaaatgcc tgaaccatcc ttaccaagta tagctatatt agctgcacca gggtcactat
     8341 tattgtctgg atatattaat tctttttcag ataaaaataa tgtagtatta tacttgttat
     8401 tcatattatc aattgtattt tatattatag ttttgggata tttaccgaaa ttattaaggt
     8461 tgccttttta tccgagtttt gctgcattta cattccctat ggcaataagt gcaatgggta
     8521 tgaaactaat gaatggttat ttaactaaat caggtcattt tataagttgg atttcatatg
     8581 cttctaagtt agaagagata attgctacag tgattatttt atatgtttta gttttgttta
     8641 ttatatatct tagtaaatct gaaactacat ctaaagctaa ataagaatta ttaaatatgt
     8701 gtatttaata ggaatattga tataaatata tgaagataag ataaaccata gaaacttaat
     8761 ttagcttaat tttattggag gaaaacaaat gaaaacaaaa gaagaatatt taaaagaagt
     8821 tagaaacata tcagaagaat actttagaaa aggagagttt ttttgctctg aagcagttct
     8881 ccagacaata aatgatgctt taggccaacc tttaagtcca gagataacta agttagcatc
     8941 aggatttccg ataggacttg gaaaggcaca atgtctttgt ggagcaatat ctgggggaga
     9001 gatggcttta ggtatagtat atggaagagt tcatggtgaa gctatgaatc caaagatgtt
     9061 tgagcatgct aaaggtttgc atgattatat taaaaaagag tatggtgcaa catgttgtag
     9121 agctattaca aagaaatggg atggtgataa ctttatgtct cccgaaagaa aagagcactg
     9181 tattaaaata acaggtcaag ttacagagtg ggttgcagat aagcttatag aagatgaaaa
     9241 acttaatatc aaatagtgta atattagttg gtgaatttat aaagttaaat ttacttatag
     9301 tataagatac ttgatattct cgtattgata gaaaaattta tagcatatgt atttaatgaa
     9361 atatacttat atttagagtg tggtttaatt tcataaatac atatgctatt attttaagta
     9421 tataagcaaa tttatttaaa taaatagaaa cagttaattt atatgtacaa aactatatat
     9481 gattagtgta aataaatacc tattttataa tactcaacat aaagacacca gacatgatta
     9541 gtattatacc aataaaaata gcaagccatt cttttttgtt cttcttttct ttaaaaaata
     9601 taatccctcc taaagtggct actatcacac aactctgaga gatagaaaaa cttattgtaa
     9661 ctcccaatgt attagtcgtt atgaacatac ctaaatttgc tattgaccac aatataccag
     9721 taattaaatt gaataaaaca ttttccctat atacaatctc tttagaaaaa tacttgacaa
     9781 taatagatga agttatcaac ataccaacag attgaggtaa tataattcta tatccttcaa
     9841 ttaaaaatat ttgattagtt acaacatata atgataaaaa tagagatgat aataatacaa
     9901 ctccaacaca ttttaacata tttttatctg tagatttttt agtttgataa tttgtgagaa
     9961 gtataccaag tattatacat aatattgata aagaaccaat cagtaacatt ttagaactgg
    10021 tccattcatt aaacaagatt acagctaata atgtcgtaaa agttaattgt gaaccatttg
    10081 aaattggcat tactttagat acactagcaa tttgaattgc tttaaactgc attaattgtc
    10141 ccaaactcca gaatatgcca gaaatcatac ttatacaaaa tgaaaaaaat gtaatttcag
    10201 gatgtataac cagagttatt atagtggaaa atataaacgc acttatggat gtacctaaaa
    10261 gctgttcatg aggagaactt ttctttagat ttgcaactat gggcatcaat ccccatccca
    10321 ttacaggaag taaagcaata attattgtca gcattaaact atttcctttc atattaaatt
    10381 tagacagctt ttaatttatc atctaaaatt aaagtgttca atacataaaa attaccgtat
    10441 ttaatcagga aatattagta gttttttaga aaaacatttt ccattaaaat aaagacaaag
    10501 ggaaggagta aaattgaaaa tgttaactga tatgattcaa aatcgtgaaa ttaaaataat
    10561 aaagttttta ttaaaagaag gtcatacaac tgtaaaagca atagcagatg atttagatgt
    10621 ttcagaaaaa acagtttcaa aatctcttaa agaagtagaa gcagtgctta aaaagatagg
    10681 tctttcttta ataagaaaac ctaagatagg tgtttatatt gatggagatt taaaaaaaat
    10741 acttccttac ctagataata aaggaagtca aattcctaca accagagagg agagagttat
    10801 ctatatatat tcagtacttt taaagagtaa tgattatatt actatacaac aactttctga
    10861 tgaactatat ataagtcgtg gaactattga aagggattta gcagaagttg aaaaactgct
    10921 aaaaaacgaa gggatttcca tatataagaa acctagtaag ggaataaagt taaatataag
    10981 tgaaagagat aaaagacaac ttactgctaa gtttatacat aaattttgga gtagaaactg
    11041 gtatattaag caagagcaag aaagctttta tcaagcattt gaaaagatac aagcagatgt
    11101 aaatggtatt ttttcggaag atgatttatc aataattatt gatattctta ggaatttcag
    11161 cagagaaaga aaatttgagt tctctgatta tgaatttcag tcattagtta ttcatttggc
    11221 aatagctgta gagagaatta gaaaaggcga atatatagaa gaaagtttgt atactggtaa
    11281 tatagaaaat aatcaaatgt caaataccaa ttatttagca aaacaattag aagaaaaatt
    11341 ttcaatatta ttaccaaaat ctgaaattgg atatataaat ttacatttag tagcgtctga
    11401 ccaacataga gatactaatg aactaaaaaa tacttcaact aatccttgtg agacaagtac
    11461 aaataatata tcaaatcttc gggaaattat aaaaaataca ttgtatgaga cagcttatga
    11521 caaggattta attgatggat taacttcaca tgtacattca tcaataaata gactgaaata
    11581 tggacttagt attaaaaatc catatgtaaa tacaatcaaa caaaactttt ctttatcatt
    11641 tgagcaagct ttagatttaa agaaagtaat tgaatttata tatgatatta ggataaatga
    11701 tgatgaatgt gcttatatag cattacattt tgaagctttt agagaaagaa tgaaagaact
    11761 tccacaaaat attagagtaa ttctagtatg cagtacagga ttaggttcat ctaggttatt
    11821 gtctgcaaga ataaaaaaat actttccaat gataaagatt gaaaaagtac tttccgtaca
    11881 agaattagta tccagtaaaa ttgatgcaga tttagtaata agtactattt atttagagtt
    11941 agaaacaatt ccaaccatag tagttagtcc tattttaaat gaaagtgata taagaacctt
    12001 ggaaagaaat atacaaaatg tttcaagtta tacttctaag aagtataaat ctttcagaaa
    12061 cttaatcttt gaagaaaata tattgttgaa tattgaagct tcaaactatg aagatgcaat
    12121 taagtatata acagataacc taataaaaaa aggttttgca aaagaaggta tagctaaaag
    12181 tgcaattgag agagaaaatt tatcttacac ttctttccaa tcaatagcta cacctcatgc
    12241 taatcctagt tatattaaaa aatctaatat atctgttgca acgttaaaaa atcctattac
    12301 atggggaaat gaaaaggtgg aagttatttt ctttatttca ttagaaaatg acaaaaacct
    12361 agagcttgat gaaatatatg aagtttttta tgacttcatt gacaataaaa aaaatataaa
    12421 tgacatatta agtgcaactt ccacaaagga agtgtacgat ttgctagtgg aggagagtat
    12481 atagtgaaaa tatcagatgt attgaaaaaa gagcaaattg tttttaatct tgatacaaat
    12541 acaaaggaag aaacaataaa aaaattaagt agtatatttg ttgaaaatgg gattgtggac
    12601 aatgaagaag aatatgttaa gtctgttttg gaaagagaag aacattcaac tacaggtatt
    12661 ggtaatggta ttgctattcc tcatgggaaa agtaatgctg tcaaaaaatc ttctataata
    12721 tttgcaaaga ctaagcataa gattgaatgg gatgctttag atgaaaaacc agtagatagt
    12781 attttcttat tggctataag caatattgat aaggatagca atcatcttgt actattatct
    12841 aaattagcaa ctaaacttat ggatgatgac aatgtagatg cactaaaatt agccaattca
    12901 gaacaagaaa taatcaatat atttagtgaa gaaggagaga tgtagaaatg aaaagaaaaa
    12961 ttatagcagt tacagcatgt gcaacaggag ttgcacatac atatatggca gctcaagcgt
    13021 taaagaaagc ttcaaaaaag atgggtcacc ttataaaagt tgaaacacaa ggagctacag
    13081 gaatcgataa tgagttaact acaaaggatg cacaaattgc agaagtagta atatttgcag
    13141 tagatactaa agtcagaaat gctgaacgtt ttgagggaaa agaaatacta gaagtaccag
    13201 ttaatgcacc tataaaggat gcagagaaag taataaatga tgcactaaag ttaattgaca
    13261 ctaaataaat ggagggaaat gtaatgttga aaaagatagt ctctgagtta aaaaagcatg
    13321 tcttaactgg aatctcatat atgattccat tagtaattgc aggtgctatg ataatggcga
    13381 tatctcgtgt aggtggttct ttctataata taccagatat atgggatgct aaatatgcag
    13441 aaagtgctag tagtattgtt agattattac atgatttaga tgggtttgga ggtactgcat
    13501 taggattaat gtttcctgta attgctgcat ttataggatt ttctatagct gataaattag
    13561 ctattgtacc aggtcttgtt ggaggtatga ttgctaaaga tataggtgca ggatttttag
    13621 gagctttggc agttggtctt atagcaggat atacatgttt gtttattaaa aatcatgtta
    13681 aattaccaaa atcagcagct tcaatagtac caatatttat agtaccagta tttgggacat
    13741 taatcactgt tgtattaatc aattatgtga taggtatacc ttttgccaca ttaaatactg
    13801 gattagaaaa ttggttaaat ggattatctg gtacaaatca gatactaatg gcagctatta
    13861 taggggctat gatggcagtt gacttaggtg gaccagttaa taaagcagct ttaacaacat
    13921 cacttgcatt attaacaagt ggaatttatg cgcctaacac tgctgcaatg gtaggaatag
    13981 ttataccacc actaggattg ggacttgcta ctatactagc aaaacaaaaa tataataaac
    14041 agttacgtga agctggaaaa tcatctctta taatgggttt aattggagta agtgaaggag
    14101 caattccttt tgcggtagaa tctccattaa aagtaatacc ttcatgtgta attggaacag
    14161 caattgcttc agctatggca gttggtttag gttcagtaaa tgcaactcca attagtggat
    14221 tttatggatg gtttacagta gagaattggc caatgtatgt gttatcaata gcagttggaa
    14281 cagtaattgt agctggatta gttatagtat tgcgtggaaa tccagtgatt gaggatgatg
    14341 aatttgaaga tgatgatttt gatgaagcag agtgggaatc ttaaaattta ataactattt
    14401 aagacgtgtt ataagctata ttcaatatga atcttatact aaataaagtg ttaaacattc
    14461 agtttagact taaatataaa agataaagaa cagaggagaa taaatgataa aagcacatat
    14521 tgttaatcat acacactggg atagagaatg gtattttact tctggagatg cattagtact
    14581 tagtgaacaa ctatttacag atgtaattga tgaattagaa cgtaatccag atgtaagttt
    14641 tgtacttgat ggacaacttt ctattttgga tgattatgta caattacaca aagaaaaaat
    14701 agaaatcatt aaaaagttaa taaaagaaga tagactacat attggaccat ggtttactca
    14761 aactgatgca ttctttgcta gaggagagtc tattttaaga aacttaatga ttggaatttt
    14821 tgaaagtaaa aagtatggta agtatatgaa aattgggtat ttgccagata catttggatt
    14881 taatgcacag atgcctatac tattaaagca tgtaggtctt gataatatat tcttttggag
    14941 aggtatacat cttggtaaac aggttcaatc accatatttt aagtggaaaa gcttaaatgg
    15001 agaaagttat atatatgcag taaatatgcc acatggatat ggaactggta tgcttttaga
    15061 gccttcatta aagtatgtta atggacgctt agacccagct attgatttta ttaaatcata
    15121 tactgatgta gatgaggtgt taataccttc tgggaatgac cagttaaata taataggtga
    15181 tttcaaaaat aaaattaaag cgattaatga tatcggaaag tattcatata taacgagttc
    15241 atatcaagag tttttaaaat atataaagtc catagaatca ctagaaagct atagaggaga
    15301 atttagagaa cctgtattag caagaataca taaatcaatt ggttctagta gaatggatat
    15361 aaaattagca tgtgataaac tagaaaacaa gctgattaaa agaatagagc cattattagt
    15421 aattgctaaa aagagtaaaa ttgaaataag caatcagtta cttataaata catggaaaaa
    15481 acttttggaa ggacaagcac acgatagttt agctggatgt gtaactgata ctgtaacaga
    15541 tgatattctt caccgtataa gagaagcaaa tgaaatatgt gacagtattg aaaatactat
    15601 tgtaaaaaag atttcagaag ggcttaaatt atctaaaaat gatattttag tatttaatac
    15661 agaagctaag cgttttaatg gatataaaga gatacaagtt gtaagtgata gtaaaaatat
    15721 ttattttaaa gataatttag atgctactat aattgaagaa acttatgtaa aaccgagaga
    15781 aaatgtatta gaagaaacac ctgctggtaa tatatttatt gaagaaccag gatattatat
    15841 attaaaagta agaataaatg tacagcttcc tgcacttggt tataaagttg ttagttttga
    15901 atcaagtgat aaagaaatgg tgtcacttaa taaatctaat gatgctttta tatctaatga
    15961 taattataag ataatatatg aagatggaag cttaaattta caacttaaag atggaactta
    16021 catatcaaac tttttaacat taaaagatag tggtaatgca ggtgatacct atgacttttc
    16081 tccactaaaa aatgatgagg atataaaact ttcgtttgat aaagtgtata cagaaaaagc
    16141 tttaggatat gaaaaaatgg tggttaaagg tactacttta ctaccactta cgttagaaga
    16201 tagaaaaaac aaatatctta atgggaaatt aaatgtaaaa gtaagtattg tcttatccaa
    16261 agaaaatcca ttgatggatg taaaactttg tgttgataat acgatatata atcatagact
    16321 acgtgttcat ataaaaactg atataaaaga taataaaaac atagcatcat tgccatttgg
    16381 atatataaca agagaaaatg gagttttaga caactgggaa gatatttaca gtgaaatgcc
    16441 tatagattta gaacctttgg aaagtaatat tactctaaca aaccaaaata gaagctgtac
    16501 tatatttact aaaggaataa aagagtatca acatattgaa gatgaaattg cattgacctt
    16561 acttgctact acagatgaac taggaaagcc tgatttatta tatagaccag gacgtgcttc
    16621 tggtgataca actaaaaaag gtcacataag aattaaaaca gataaagctc aaatgcttaa
    16681 gaaattagaa ttttcatttg caatatatat ggattctaaa tcatttgatg aattggaaat
    16741 tgcgaattta acccacaatt atctaaggga atgtgtaaat tatcaattac aggattacaa
    16801 tttcttttta tatcgtatag ataataagat tcaaaaatca attgtcaata aaactgtaag
    16861 tagagaatta gaaataatat ctttaccaga aaactatctt atatcagcat gttatccttc
    16921 ttattacagt aaagataaat ttataataag agtggaaaat ccaagtagta ataaaatgat
    16981 attggatgag caaatattta aaaatagaaa tgggaaaatt gtaaatgcaa tagaagatgt
    17041 agaagaggaa caggtatatg aaatagcacc ttttgacgtt atttcaatat tattggattt
    17101 ataagtgtaa aagtaagttt attgtttaaa gtctgacaga tattaatctg ccagactttt
    17161 taatttgtat aaatttgggt aaatattaat gaatggtttt agtatttaag tatgaattta
    17221 gtatttaata tagaatttat tgaagtagaa taattagttt aacaggataa gcagtttaaa
    17281 taaagaatta t
//
LOCUS       DAMJYR010000081        17091 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.082, whole genome shotgun sequence.
ACCESSION   DAMJYR010000081 DAMJYR010000000
VERSION     DAMJYR010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 17091)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17091)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17091
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.082"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(584..1318)
                     /locus_tag="PC471_002549"
     CDS             complement(584..1318)
                     /locus_tag="PC471_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDF2789668.1"
                     /translation="MNLAKGLDVLKISSNVFGEDKVMYIPVIYTEDDATLIDTGLPGQ
                     GDLIIDALNKSNTSFDKLKNIIITHHDIDHIGNINYLREKSKNNIKVYAYKSEISYIT
                     GEETPFKLYMLEQMVDKIDDKMLSMLNVMGLGFKSSYTKVDVSLDNHEKLNLGEEIEV
                     IHTGGHTRGHICLYLKESKVLIAGDLLQVENGELKPVDVMHSNKQELKDAIKNISNYD
                     IETIVFSHGGLYQRNIIGTLKNLIIE"
     gene            complement(2329..2967)
                     /locus_tag="PC471_002550"
     CDS             complement(2329..2967)
                     /locus_tag="PC471_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 transporter permease"
                     /protein_id="HDF2789669.1"
                     /translation="MLIHLVKKDILIVKKYVLLILFIAIGIPLFTLWRLPPFAQILGF
                     VLSIIFSEFMLCQYLLLKESQYSKASVLLCSTPYPRRGLVQSKYVLFAMVFIYCTLVY
                     WIENLLIPQIGTFDLTYILIVLLISSIIYGVYMPIQYKLGYETTKFFFVVIIMASSFV
                     LPLTIAHSNFEIPFIYQYPSMIVNIVLFLLSLIILLASMFISIKIFSNKELV"
     gene            complement(2970..3827)
                     /locus_tag="PC471_002551"
     CDS             complement(2970..3827)
                     /locus_tag="PC471_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2789670.1"
                     /translation="MENILEVYNLNKTYTDFSLSNISFALPEDCITGFIGVNGAGKTT
                     TIRSILDVVKRDSGKIKIFGKDFDGNEKELKNRIGVVFDDSYFYEELSMAEMKSIIAP
                     SYDNWCEEDYQQNMKRFNLNPKQKIDTLSKGMKMKFALALALSHKAEFLIMDEPTSGL
                     DPLIRSQILDILTGYMKKCGRGVFFSTHITSDLDKIADMIVMIDNGRIILEREKDTLL
                     DEHRKVKGDIQFLNDEIKKLFLNLEITGYGFIGITSKINEVQKNMPDILIERPTIEDI
                     MLAYIERRE"
     gene            complement(3829..4203)
                     /locus_tag="PC471_002552"
     CDS             complement(3829..4203)
                     /locus_tag="PC471_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDF2789671.1"
                     /translation="MKVLISNTSDKPLYQQIKEQIKEAIFTEELKEGDAMPSIRNFAN
                     DLKVSILTIRRVYEELENEGFLTRQVGIGTFISTGNLEILRDSKRRIVEQKMADMIKD
                     AKALGITQIELQEMMDILYQED"
     gene            complement(5365..6555)
                     /locus_tag="PC471_002553"
     CDS             complement(5365..6555)
                     /locus_tag="PC471_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HDF2789672.1"
                     /translation="MSDMLLWFSVVLVLFAIGDLIASKTKAKVSAVFVTLLLFLILFV
                     TKAIPADIIEKAGMTAAASWSVPMIMFSMGTMLNVKQFIDEWRTVLTAWLGIVAVIVC
                     VSLCIPLFGKSTVLTSIPVINGALPATTIMTQAALEKGLTLAAATATVVFAIQKFIGT
                     PIASRAALQEANRLLVEYHEAKSKGIDLANVDTDKKEAENTGTKVKQAFCEKYDKYYS
                     TNVCIFFIALFSYLGYELSEIIHVNYSIVCLVVGVIVTRIGIVPKDILEKGKIKGFIN
                     MVVFAAVIPSLAKVSLTDLISLFVPIVGMFAASIIGIFLMMKVLPGWKIIGSKPLAFG
                     VGFCQMLGFPTTYLISNEVCNAVGETEEERAYLMSKIMPKLVVGGMACMISIVVAGLM
                     VPML"
     gene            complement(6571..8526)
                     /locus_tag="PC471_002554"
     CDS             complement(6571..8526)
                     /locus_tag="PC471_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HDF2789673.1"
                     /translation="MEKIKIESSDIFEYIFPHDISCSPDGKHIAYIISNINEEKDCYE
                     HDLYVMDIKTEKQIHMTQTKDVTSFSWISNTELLFTSKRNKPKAGTTDFYTISIEGGE
                     AKKAFSIPKACSVPVSLGNKLWLLTTKNPTDSKKSEPDRAVEGVDYWTFTDKPFIRDG
                     ENFSQRRRVTLELYQEGENITKAITPKFCEVAGIDVSSDKNRILYTGQIYEDCATPFS
                     GLWEYHIDSGETKELVPQGKYQISLAKYIGKDKVMLQASTLDRSITQNHDIFILDLST
                     SEINMIASPDGMYATLLDVDAVYGGGRSNKVIGDKFIGARICRTMTEFNEFDTKTGNI
                     RIITKVDAFTSFDIYDNTMYTVMLKDYELAEIYSIDMTTGTMKKMTAFSKPYLDTHKV
                     SLPEKLTFVAKNKEEVDGFVIPPIDAKEGEKYPAVLFIHGGPKWAYGYMFTHLKQCVT
                     SKGMYVIYCNPHGGDGYGEKFLEMVERWGYVDYEHLMEFVDTCIEKYPGIDADRLGVA
                     GGSYGGYMTNWIIGHTDRFKAAVSQRGISNLITASLIIDFGDRIMKQTCGDKTPWNHE
                     EVLWNHSPIKYVKNVKTPTLFLHSDRDYRCFMGDTFQMFTALKQLGVDTEMYLFHGDT
                     HGLSRNGRPSNRIARANAIVDWFERYL"
     gene            complement(8543..10609)
                     /locus_tag="PC471_002555"
     CDS             complement(8543..10609)
                     /locus_tag="PC471_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HDF2789674.1"
                     /translation="MKEVVGSLKKITKERIENTMKKLFTSETFLEYQFISDCQISPDG
                     GYTAFIVKKADIKENGYTSQVYILNNKTGELKQITSINSVGAYAWEDENTILFPALRN
                     EKVKEAVKNGKQCMSYYALSLNGGEAEELFRLPIKGGKLNPIGKGLYAVIDSYDNDRP
                     IVEGLPEEEQQKKINAYNKRHYEHFKEIPYAVNGEGYISRKRKRLYVYDSNINELKAI
                     TAPMFNVVGMKISDGKILYIGQEFKDVKGLKNGVYVFDTNTNTNVCILEKDKYIIKGF
                     ELYQNQVILNLTDALSYGNGENGDFYTIDIDTKEMKLLSGHQHHCIGNTVTSDVKMGA
                     GQTTKVDGEYIYYTSTVDMDCIIERIHIPTGKQEKVTQTGSVDFIDVKDGNIVCVGCL
                     GNGLPEVYTVENGTLCKKTHLNDHILEEYKISVPEYIESKGSSKWEIQGYVYKPVDYE
                     VGKKYPAVLAIHGGPRLTYGPYFMHEIQVFTSAGYFVFFCNPRGSEGRGNAFADIRKQ
                     FGEIDYIDFMEFTDTVLEKYTDIDKTKLAVEGGSYGGFMTNWIIGHTNRFAVACAQRS
                     IANWSGMEGTTDIGYYFCKGQTGASHMENHELQWKQSPLAYVDKCVTPTLFLHGEKDY
                     RCYMQEAFQMFSALKIHGCPTKLCLFEGENHELSRSGRPKQKLQRLVEMLDWFSVYIK
                     KDNIGQ"
     gene            complement(10975..11625)
                     /locus_tag="PC471_002556"
     CDS             complement(10975..11625)
                     /locus_tag="PC471_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDF2789675.1"
                     /translation="MKVKSNLDEILYHKIIESLIRGEYSVGQKILLNDLCEKFEVSRT
                     PVVQAVKMLNKDGVLTIMTNGKVYVPEYEYDMVKQVCETRTLIETYALEKMMQEEEEV
                     FQQKLDVIKRYSDKCEEYYRQEKSVELALADLELHKAIVEGANNEILNDVYVGIQGRF
                     IVVNYLIRPLKNRNYEGTVQDHFEILKFIEDRDTKKAVDKLRNHIQGTIKRFCEDE"
     gene            complement(12609..12944)
                     /locus_tag="PC471_002557"
                     /pseudo
     CDS             complement(12609..12944)
                     /locus_tag="PC471_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427885.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
     gene            complement(13042..13722)
                     /locus_tag="PC471_002558"
     CDS             complement(13042..13722)
                     /locus_tag="PC471_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2789676.1"
                     /translation="MIHLMKLELKKVGLKRYFLFSMLGIFLCMFFVFTGLNDKSTSIY
                     DYDVTFRTISLFFCFYYIILFAVLNVAYIINEYTNKTILILFSYPLDRKKLILSKLLL
                     ITTLMISSMIVGYICCFTFVVVLDKYFNLVIGDFHISILSYWIPGAIKSIIVFCSLGI
                     WTFVVGMIRKSIPVTLVSSIIFINLRQFILAGTNTTQDSLISVFIIVGITVIGIYYIL
                     NHKVTEID"
     gene            complement(13719..14645)
                     /locus_tag="PC471_002559"
     CDS             complement(13719..14645)
                     /locus_tag="PC471_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2789677.1"
                     /translation="MNYIIETNQLKKIYKDKAVVNAVNIHVKKGEIYGFVGPNGAGKS
                     TVMKMLLNLVKPNSGEIVMFGKKVAETDFEILKKIGTIIENPYLYENLTAKQNLDLHC
                     EYMGYYNKEHISEVLECVGLSKQSNKKVSKYSLGMKQRLAIARAILTKPELLILDEPI
                     NALDPEGIREMRELFRKLNTDYGITIFISSHILSEVEQIADTIGIIQDGKLIKEISMS
                     DIHKYQTDYVEVDVDNVELAGYLLEKEFGIKDFKITSESCIEIYDLRKDVKEISKIFI
                     QNQIGINSIGRKQSSLEDYFFQTTGTGGKETL"
     gene            complement(14718..15635)
                     /locus_tag="PC471_002560"
     CDS             complement(14718..15635)
                     /locus_tag="PC471_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HDF2789678.1"
                     /translation="MELFLVVISIVLFALYLKERNKSKKMESDYKYINSRLRDITNDD
                     EINYILVPSDISIVKETAVDINKLLEKFYSRQINYNRSEKAMLQIFTNISHDLRTPIT
                     VLKGYIEMLYLQSQKEELSSSMRATVEKMQTNSNELVNSINNLFNMAKIQSGDMILNL
                     QKSNLTQLCHEIILEFYDLLDKENFNVEVNIEDRPIYANVDTEALQRILKNLIDNAIK
                     YGSDGRFLGISLYEKEKHVFIDIEDKGSGISETEKEHIFTRAYTADRKKGNGLGLAIS
                     QGLVNSMGGSIYVNSRPNVKTVFTVKLKS"
     gene            complement(15635..16324)
                     /locus_tag="PC471_002561"
     CDS             complement(15635..16324)
                     /locus_tag="PC471_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2789679.1"
                     /translation="MDILLVEDDKNIREMVYDFLISENYSVKTAIDGNTAIEIFKNYS
                     FDIILLDLMLPKSSGFEVLKYIRSVSVVPVIITTAKDSDTDKMLGLNLGADDYITKPF
                     SIVELLARIKANIRRATVYSSSRNSEDMVIQYKDLIINISKYVVSKNGIDLNLTHTEF
                     EIVKLLATNQGRAFSKEQLYNAIWKEPYYGNENVLNTHINRLRNKLSDGIEKNNYIKT
                     LWGIGYKMEEE"
     gene            complement(16732..17037)
                     /locus_tag="PC471_002562"
     CDS             complement(16732..17037)
                     /locus_tag="PC471_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2789680.1"
                     /translation="MPSMIMEVVGVLFIAIGLILTFIEIKGNFPSFYGFGQNSIGKNA
                     VGIIAAGIALIFFRNGIDSIDSIATYGVFLAIELVVIVAYFLIRKKFTKEDKHLRTN"
BASE COUNT         5860 a         2879 c         1872 g         6480 t
ORIGIN      
        1 atggtttaaa taatcttctt tactttgtat tatgatacat gctttaaata tttttacgca
       61 ctttatatta taatacatgc tttaaataat cttattcgct ttgtattata acacattatt
      121 taaatatttt tactcactct gcattatagt acattattta aatatttttc ttcgctttgc
      181 attatgatac attatttaaa tatttttact caccttgtat tatggtacat tatctaaata
      241 tttttcttca ctctgtatta tgatatataa cttaaatatt ttacctcact ctacaatatt
      301 atatataatt taaatatttt tcttcacttt gtatactaaa gtttatttaa agtattttct
      361 ttcccattat actatgctgt taaatatttt tcaactgaag tattgtagaa ccttaaaatt
      421 actataaaaa actatattct aaaatacttg taatactacg tatcaccctt aacacttaag
      481 tataatttta aagcaaaatt cttattactt atatttaatt ttgattctac atctttagat
      541 ataattttaa gtaatttaag aaattattgt aaatcatata gtactactca ataattaggt
      601 ttttcaatgt tccaattatg tttctttgat ataatccacc atgactaaaa actatcgttt
      661 ctatatcata attacttata tttttaatag catcttttaa ctcttgtttg ttgctgtgca
      721 ttacatcaac aggcttaagt tctccatttt ctacctgtaa caaatctcca gcaataagta
      781 ctttagactc ttttaaataa agacatatgt gacctctagt gtgacctcct gtatgtataa
      841 cttctatttc ctcacctaga ttcaattttt catggttatc taatgacaca tctactttag
      901 tatatgatga tttaaatcca agtcccataa catttagcat actaagcatc ttatcatcaa
      961 ttttatctac catttgttcc aacatataaa gtttaaaagg agtttcttct cctgttatat
     1021 agctgatttc acttttgtat gcatatactt tgatattatt cttagatttt tctcttagat
     1081 aatttatgtt tcctatatgg tctatatcat gatgagtaat tattatattt tttaacttat
     1141 caaagctcgt gttactttta ttaagagcat caattattaa atcaccttga cccggaagac
     1201 ctgtgtcaat aagtgttgca tcatcttctg tgtagattac aggaatatac ataactttat
     1261 cttcaccgaa tacatttgat gaaattttta aaacatctaa tccctttgct aaattcataa
     1321 tttttctcct tataattact taattagagg tcaatttaat taaagaaatt gagaaaatgt
     1381 agattttcct ctagttatat tttattaatt aaaaattatt aatacctagt atatcactta
     1441 agttgaagga ttactcgact tgattttttt gttattttat ggtatagtaa ttaatataga
     1501 agtaagtaat tgcaagcttt gcaattactt ttattttttt ctaataacta ttttattttt
     1561 gataattcgt ataaaattaa aagtaaagta atatggaaaa tatgaatata gaaaaacttt
     1621 aataagaaaa taaattatga ataaataaaa gaaacgcaat actgtatata gatgattatt
     1681 tagaaaaata aaatctcttt taataaaaat aagcttcaaa ataaactttt agttcataaa
     1741 gcttaggagg aactaaattg agtgcaagtg tatctactgt tcaaaataaa tagttcataa
     1801 agcactcttt tttatattaa atcaattata ttttcgtcat ttcagcaatg aaaaagaaac
     1861 ttatctctat tttgagacag cttcttttta ttaacttcct actttttatt agttttctaa
     1921 aattgaaaag ctttatttat aggattcctt agatttatat tttgacgata taatttttta
     1981 aaaacatctt aaaagatggt atacaataca cacaatcata ctcataattg aaaaataatc
     2041 aatggaaaat aaaattactg gatattatct attaaaaaat acaaattgat tcacaagaaa
     2101 catataatct ataatattat gatgttactc tctcgcacca agtaacttta ttttcaagta
     2161 accttttaaa ttcataccat ctaacttcac tcaataaaaa tggcaatata aatatgtact
     2221 ctatttttta cttttaaata tctgacaagc tttatatact attggaagta aaatacaaag
     2281 taaaacagcc caatctggta aatgtggcat ataaaagtcc cccttacctt atacaagctc
     2341 cttattacta aaaattttaa tagaaataaa cattgaagct aacagtataa ttaaacttaa
     2401 aaggaacaat acaatattca ctatcatgga tggatactga tatataaatg gaatttcaaa
     2461 attgctgtga gctatagtta atggtaatac aaatgatgaa gccatgatta tcaccacgaa
     2521 aaagaatttt gttgtttcat atcctaattt atattgaata ggcatataaa ctccataaat
     2581 aatagaactt attaataaaa caatcaaaat atatgttaag tcaaaagttc ctatttgtgg
     2641 aataagtaaa ttttcaatcc agtaaactaa tgtacaatag ataaatacca ttgcaaataa
     2701 aacatactta gattgaacta agccacgtct aggataagga gttgaacaaa gtaatacaga
     2761 tgcttttgaa tattgactct cctttaataa tagatactgg cacagcataa attctgaaaa
     2821 tataatggat agtacaaatc ccaatatttg tgcaaatgga ggtaaacgcc acaatgtaaa
     2881 taatgggatt cctattgcaa taaacaatat caacaataca tattttttta caatcagaat
     2941 atctttttta actaaatgaa tcaacataat tactctctcc tttcaatata tgcaagcata
     3001 atatcttcaa ttgtaggacg ctctattaaa atatctggca tatttttttg cacttcatta
     3061 atttttgagg taatccctat aaaaccataa cctgtaattt ctaaatttaa aaataatttt
     3121 ttgatttcat catttagaaa ttgaatatcc ccttttactt ttctatgttc gtccaataga
     3181 gtatcttttt cacgctctaa tataatacgt ccattgtcaa tcataacaat catatctgct
     3241 attttgtcta aatcagatgt tatatgagtg gaaaagaaaa caccacgacc acattttttc
     3301 atatatcctg ttagtatgtc aagtatctga ctacggatta gtgggtctaa accacttgta
     3361 ggttcatcca taattaaaaa ctcagcttta tgcgataatg ctaatgctaa tgcaaatttc
     3421 attttcattc cttttgaaag tgtatcaatc ttttgttttg ggttaagatt aaaacgcttc
     3481 atattttgtt gataatcttc ttcacaccaa ttatcatatg aaggtgcaat aatacttttc
     3541 atttctgcca ttgacaactc ctcatagaaa tatgaatcat caaatacaac tccaattcga
     3601 ttttttaatt ccttctcatt tccatcgaaa tctttaccaa agatttttat ttttccacta
     3661 tcacgcttta cgacatccaa tatcgaacgg attgttgttg tttttcctgc tccattaaca
     3721 cctataaatc cagttataca atcttctggt aatgcaaagc ttatattaga taatgaaaaa
     3781 tcggtataag tcttatttaa gttataaact tctaaaatat tttccatatt aatcctcctg
     3841 atatagaata tccatcattt cttgtaactc tatctgtgta attcctagtg cttttgcatc
     3901 cttaatcata tctgccattt tttgttcaac tatacgtctc tttgagtctc ttagtatttc
     3961 taaatttcca gttgaaataa atgttcctat tccaacttgg cgtgtaagaa agccttcatt
     4021 ttcaagttct tcatacactc gtctaatagt caaaatacta acttttaaat cattagcaaa
     4081 atttcttatg gatggcattg catccccttc tttcagttct tcagtaaata ttgcttcttt
     4141 aatttgctct ttaatttgtt ggtataatgg cttatctgat gtgttagaaa tgagcacttt
     4201 cattttttat cacccccttt ttatcatttt cagtgtgatg ttgttatata cacactatac
     4261 acttagagca cttttttgtc aatataaaag atgtaaaata ttactatgac aaaataaata
     4321 tgaatgaaat attagagatt gttgatagct ataaacactt tttaaaacca cataagatag
     4381 aatgtattaa atatttatta aaagtaagaa ttgaatatct aaaaaaaaga ggaatcctgt
     4441 atgaatgatt tgtttgatga taaattaagt tataaactat ttataaaaaa cataaaggaa
     4501 taaatccatt tctaattaca aacatactaa aaaatttaga tagtaataaa tagatagtaa
     4561 taaatagata gtaataaatg ggatgaattt ataagaattt ggatagagga aagagttatt
     4621 caagagtgta gactagaggt aaactctata ttaaaaaaat ttaaatttgg atattatgat
     4681 gcatggaata tttgcagaag taaccttgct ataagtatag aagatttttt tgattacaaa
     4741 agataatact ctaaaatgtg aggatttcaa cattcaatac tgtactttaa ataatatcac
     4801 tgcaaaatac catccacctt ttaccataat tgaataccct aattatttta aaaaagaaaa
     4861 cattagaaac agaaaacctt ggatatataa aataaaaaat actaacttct aaaacacgga
     4921 tactctttga taaataaaac aaaaaataat tttaataatt aaaattggct atctcaaact
     4981 agagataagt ctctttctca cttttaaaac gttgaaaata tattcgattt cgtacaaaaa
     5041 agagtgcctt agaactaaaa agttcacttt gtggcactcc cttttttctc ctctttttaa
     5101 ttttttataa aaattcttca aaaatattat taaataccct atattataca taaatcaaat
     5161 taaagttgca ttttagcaaa taaaaacaaa ttataactat tagcatagca aataaaaaat
     5221 ctgctctctt ataaaatcaa attataatta taaaaagaaa atattcttat aacaaattag
     5281 ctaatataaa attattttat aaattaatac tactttttag cttctcctat aaattaatac
     5341 cactttttta actttgaaac ctatctacaa cattggtacc ataagaccag ctaccacaat
     5401 agaaatcata caagccatac caccaactac cagcttaggc ataatcttag acatcaaata
     5461 tgctctttct tcttccgttt ctccaacagc attacacact tcatttgaaa ttaaatatgt
     5521 agttgggaat cccaacatct gacaaaaccc tactccaaaa gcaagaggtt tagaaccgat
     5581 aattttccaa cctggtaaca ccttcatcat aaggaatata ccaatgatag aagctgcaaa
     5641 catacccaca attggtacaa aaagactaat taaatctgtc aaagaaactt ttgctaaaga
     5701 aggaataaca gctgcaaaca caaccatgtt gataaatccc ttgatttttc ctttttccaa
     5761 aatgtctttt ggcacaatac caatacgtgt tacaataaca ccaacaacaa gacatacaat
     5821 agaataattt acatgaataa tctctgaaag ttcataccct agataagaga ataatgctat
     5881 aaaaaatata caaacatttg tagaataata tttatcgtat ttttcacaga aagcttgttt
     5941 tacttttgtg cctgtattct ctgcttcttt cttatcagta tcaacatttg ctaaatcaat
     6001 gccttttgat tttgcttcat gatattctac taaaagacgg tttgcctctt gtaacgctgc
     6061 tcttgaagct attggtgtac caataaactt ctgaattgca aatacaactg ttgctgtagc
     6121 tgctgcaagt gtcagtcctt tttctagtgc tgcttgtgtc ataatagtag tagcaggtaa
     6181 agctccattt ataactggta tgcttgtcaa tactgttgat tttccaaaca aaggaataca
     6241 taatgagaca cagactataa ctgctacaat tccaagccat gctgtcaata ctgttctcca
     6301 ttcgtctata aattgtttaa cattcaacat tgttcccata ctaaacataa tcattggaac
     6361 actccaacta gctgctgcgg tcatacctgc tttttcaata atatcagctg gtattgcttt
     6421 tgtgacaaat agaatcaaaa ataaaagtag tgtaacaaat acagctgata cttttgcttt
     6481 tgttttgctt gcaattaaat ccccaatagc aaataaaact aatacgacac taaaccataa
     6541 taacatatca ctcatagaat aaccccctta ttataaatat ctttcaaacc aatctacaat
     6601 agcatttgct cttgcaattc tattagatgg tcttccattt ctagacaatc catgtgtatc
     6661 tccgtggaac aaatacattt ctgtatctac accaagctgt tttaatgctg taaacatttg
     6721 gaaagtatcc cccataaaac atctataatc cctatcacta tgtaagaaca atgttggtgt
     6781 cttaacattt ttcacatatt taataggtga atgattccaa agaacctcct catgattcca
     6841 aggcgtctta tctccacaag tctgcttcat aattctatcc ccaaagtcaa taattaaaga
     6901 agctgtaatc aagttactaa tacctctttg agaaactgct gctttgaaac ggtctgtatg
     6961 accaattatc cagtttgtca tataaccacc gtaacttccc cctgcaaccc ctaagcggtc
     7021 tgcatcaatc ccaggatatt tttcaataca agtatctaca aattccataa gatgctcata
     7081 gtcaacatac ccccatcttt ctaccatttc taagaatttt tcaccataac cgtcaccacc
     7141 atgagggtta caataaatca catacattcc ctttgatgtt acacactgct tcaagtgtgt
     7201 aaacatatag ccatatgccc atttaggccc accatgaatg aacaatacag caggatattt
     7261 ttcgccttct ttagcatcta taggtggtat aacaaaacca tcaacttctt ctttattttt
     7321 tgctacaaac gtcaattttt caggtaatga aaccttatga gtatctaaat atggtttaga
     7381 aaaagcagtc atttttttca ttgtacctgt agtcatatca atgctatata tttctgctaa
     7441 ctcataatct ttcaacatta ctgtgtacat ggtgttgtcg taaatatcaa aagatgtaaa
     7501 agcatctact tttgtgatga tacgtatatt acctgtcttt gtatcaaact cattaaattc
     7561 tgtcatagtt ctgcaaattc ttgcaccaat aaatttatct cctataactt tattactacg
     7621 tccaccacca tatactgcat ctacatccag taatgtagca tacataccat ctggactagc
     7681 aatcatattt atttcactag tactcaaatc aagtataaaa atatcatgat tctgtgtaat
     7741 agaacggtct aatgttgaag cttgcaacat tactttatct ttgccaatat attttgccaa
     7801 acttatttgg tactttcctt gtggtacaag ttctttggtt tcaccactgt ctatatgata
     7861 ctcccaaagt ccagaaaaag gagttgcaca atcttcataa atctgaccag tatataaaat
     7921 tctgttttta tccgaagaaa catctatacc tgctacttca caaaattttg gtgtaatggc
     7981 ttttgttata ttttcacctt cttgatataa ttctaatgtt acacgtcttc tttgagaaaa
     8041 attttctcca tctcttataa aaggtttatc tgtaaatgtc caataatcta caccctctac
     8101 tgccctatct ggttctgatt ttttagaatc cgttggattt tttgttgtca gtaaccacaa
     8161 tttatttcca agtgatacag gtacgctaca agcttttgga atagaaaatg ctttttttgc
     8221 ttctcctcct tctatagaaa tagtgtaaaa atctgttgta cctgcttttg gcttatttct
     8281 tttagatgta aaaagtagtt ctgtattaga aatccaacta aatgatgtta catcttttgt
     8341 ttgagtcata tgtatttgtt tttctgtttt gatgtccatc acatataagt catgttcata
     8401 gcagtctttt tcctcgttga tatttgatat aatataagca atatgctttc catcaggtga
     8461 acaagaaata tcatgaggga atatatattc aaaaatatct gaactttcaa tctttatttt
     8521 ttccattttt atattctcct ttctactgtc ctatgttatc tttttttata tataccgaaa
     8581 accaatctag catttcaact aatctttgta gcttttgttt tggtctacca gaacgagaca
     8641 gttcatggtt ttctccttca aatagacaaa gttttgtagg acacccatga attttcaatg
     8701 ctgaaaacat ctggaatgct tcttgcatat agcatctata gtctttttca ccatgtaaaa
     8761 ataaagtcgg tgtaacacat ttatcaacat aagcaagagg tgactgtttc cattgaagct
     8821 cgtggttttc catatgagaa gcacctgttt gtcctttgca gaaataataa ccaatgtctg
     8881 ttgtaccctc catgccactc cagtttgcaa tagaacgctg agcacaagca acagcaaaac
     8941 ggtttgtatg accaattatc caattcgtca taaatccacc ataacttcca ccttcaacag
     9001 ccaattttgt tttatcaatg tctgtatatt tttcaagtac agtatctgta aattccataa
     9061 aatctatata atcaatttct ccaaactgtt tcctaatatc tgcaaaagca ttgccacgac
     9121 cttcagaccc ccttgggtta cagaagaata caaagtaacc tgcacttgta aatacctgta
     9181 tctcatgcat aaaatatggt ccatatgtca atcttggtcc tccatgaatc gccaatacag
     9241 ctggatattt tttccccacc tcataatcta ctggtttata tacgtatcct tgtatttccc
     9301 atttagaact tccttttgac tctatgtatt ctggcacaga aattttatat tcctctaaaa
     9361 tatggtcatt taaatgtgtt ttcttacaca atgttccatt ctctactgta taaacttctg
     9421 gtaatccatt tcctaaacaa cccacacata caatattgcc atcttttaca tctataaaat
     9481 caactgaacc tgtctgtgtt actttttcct gttttcctgt tggaatatgt attctttcaa
     9541 taatgcagtc catatctact gtagaagtgt aataaatata ttctccatct acctttgttg
     9601 tctgaccagc acccatttta acatcagatg ttactgtatt tccaatacaa tgatgttgat
     9661 gccctgaaag aagtttcatt tctttcgtat caatatcaat agtataaaaa tctccatttt
     9721 ctccatttcc ataagataaa gcatcagtta ggtttagtat cacttgattt tgatataatt
     9781 caaaaccttt tataatatac ttatcttttt ctaatataca gacatttgtg tttgtgtttg
     9841 tatcaaatac atagacccca tttttcaaac cttttacatc tttaaattct tgccctatat
     9901 ataagatttt tccatcactt attttcatac ctacaacatt aaacataggt gctgtaattg
     9961 ccttcaactc atttatatta ctatcgtata catataggcg ttttcttttt ctgctaatat
    10021 atccctcccc attgacagca taaggaattt ctttgaaatg ttcataatgt cttttgttat
    10081 aagcatttat ttttttctgt tgttcttctt ctggcaatcc ctctacaata ggtcggtcat
    10141 tgtcataaga atcaataact gcatacaaac cttttcctat aggattcaat ttaccacctt
    10201 tgatagggag tctaaaaagt tcttctgctt ctccaccatt taaagataaa gcatagtaac
    10261 tcatgcattg cttaccgttt ttgacagctt cttttacttt ttcatttcgc aaagcaggga
    10321 acaagatagt attttcatct tcccaggcat atgctccaac agagtttata gacgttattt
    10381 gttttaattc gccagtttta ttgtttaata tataaacttg gcttgtgtaa ccattctctt
    10441 tgatatcagc tttttttaca ataaatgctg tatatcctcc atctggtgat atttggcaat
    10501 cacttataaa ttgatattcc aaaaatgttt cactcgtaaa tagctttttc atagtatttt
    10561 ctatcctttc tttagtaatt tttttcaaac tccctaccac ctctttcatt ttttatttta
    10621 aatttaaaat aaaaaatttt aaatttaaaa taaaaaattt tataaaggtt atttttttag
    10681 gaagccttct ggcttccttg taatatttgt aatttaaagt atatttaatc catgcttaat
    10741 tcatgcttaa ttcatactta atctatactt aatctatact tagtccatac ttaatctata
    10801 tttaattcat acttagactt atttacacta tatttagatt atatttatta gattatattt
    10861 attagattat atttattaga ttatatttag attatgttta aactataatt agattatact
    10921 tagactacat ttagcaactt aatagataag tatatatagt aacttcaaaa cttattattc
    10981 atcttcacag aatcttttaa tagttccttg aatatgattt cttagtttat ctactgcttt
    11041 ttttgtatct ctgtcttcaa tgaattttaa tatttcaaaa tggtcttgta ctgttccttc
    11101 ataattacga ttttttaatg gtcgaattaa atagttcact actataaatc ttccttgtat
    11161 accaacatat acatcattta aaatctcatt atttgcccct tctacaatgg ctttatgtaa
    11221 ttccaaatct gctagtgcaa gttctacaga tttttcttga cgatagtact cctcgcattt
    11281 atcagagtat ctttttatta catctagctt ttgttgaaaa acctcttcct cttcttgcat
    11341 cattttttct aaagcatatg tttcaatcag tgttctcgtt tcacaaactt gttttaccat
    11401 atcatattca tactctggta cgtacacttt accatttgtc ataatagtaa gcactccatc
    11461 tttatttagc atttttactg cttgtacaac tggtgttctg cttacttcaa atttttcaca
    11521 aaggtcattt agtaatattt tttgacctac agagtattca ccacgaataa gactttcaat
    11581 aatcttatga tataaaattt catctaaatt tgactttact ttcactactc tcaccttatt
    11641 tctattcttt tgccatatta tattgaatta ctggacttta attactttta gtaatatttt
    11701 taaagtagaa attcttattt gatagtactt ttgtactaca atttaaatta ttgtactgta
    11761 aatcatgctc ttatactata gattaattca ttgtattgca aatcaagtta ttgtactata
    11821 gattaaatcg ttatacttca aattaaacta ttatactata gattaaacca ttgtacttca
    11881 aattaaacta ttatactata gattaaatcg ttgtacttca aattaaacta ttatactata
    11941 gattaattca ttgtacttca aattaaacta ttataccata gattaattca ttgtattgca
    12001 aatcaagtta ttgtactata gattaaatcg ttgtacttca aatcaagtta ttgtactata
    12061 gattaaatcg ttgtacttta aattaaacta ttatactata gattaattca ttgttctgca
    12121 aatcatgcta gtgtactaca aattaaacta taaatgttta ctatctctta ataatttgaa
    12181 taatatgtta caaaaagtat agcacctttt tatttttttc gcaataaata aaaaatttaa
    12241 aattttttat tttttatttt cttatattat caatttgaaa tatgaaagat taagactaat
    12301 agaattgatt cagttaattg aaaaaaatag gtacctttta tgaaaattct aatgataagc
    12361 atttttctga tgtaatggta aattataaat gataaaatta aaaatcactt ttagttttat
    12421 gaactgtccc catattaaga aacaactaag atacataaaa atagttataa agagatagtt
    12481 aaactatatc cactatttta tatagttaaa atagtgggta tagtccaagc taagaataac
    12541 taagaaatta gcgttaaata gcatagataa aaatacattt atatataaac tttttttgct
    12601 gcaaacaatt atttccataa cattttatat acttctctaa aatcttctaa acttgataaa
    12661 tattcaatta aagggacata attacctaaa ctacaagaat ttaaggagtt tctatacaat
    12721 tcctcactat cccaagatac aatttttata ggaagattat tttctaacat ctgttttagc
    12781 ataacaaatc tacctatgtg accattacca tcttgaaatg gatgtatcaa ttcaaatcta
    12841 taatgaaatt cagttataac tttaatagat acttcactct gactatagta ccattcaatt
    12901 aattcatcaa gctttggctc aacttcaaat ggttgtgcaa tattattaaa actctaataa
    12961 tattaataat agctgatata atagcaaatc attattatcc cgagaagata caattaataa
    13021 ctaccttcct atccacagta tctagtcaat ttcagtaact ttatgattta gtatataata
    13081 tataccaatc acagttatac caactataat aaaaacagaa atcaaactgt cttgagttgt
    13141 atttgtacca gctaaaataa attgtcttaa attgataaaa ataattgaac tgacaagggt
    13201 aacaggtatt gattttctta tcattccaac aacgaatgtc catataccta atgagcaaaa
    13261 tacaataata cttttaattg ctcctggtat ccaataagat aaaattgaaa tatgaaaatc
    13321 tccaataact aaattaaaat acttatccaa cacaacaacg aatgtaaaac aacagatata
    13381 tccaactatc attgatgata tcataagagt cgtaatcaaa agtaactttg ataaaataag
    13441 ttttttacgg tcaagtggat aagagaataa aatcaagatt gttttatttg tatattcatt
    13501 tattatatat gccacattca acacagcaaa taatataata tagtaaaaac agaaaaataa
    13561 actgattgtc ctaaatgtaa catcataatc atatattgaa gtagatttat cattcaatcc
    13621 agtaaagaca aaaaacatac ataagaaaat ccccagcatt gaaaataaaa aatatctttt
    13681 tagcccaact ttttttagct ctagtttcat taaatgtatc atagtgtttc tttacctcct
    13741 gtacctgtag tttggaagaa gtaatcttct aaagaacttt gtttacgtcc aatgctattt
    13801 ataccaattt gattttgaat aaatattttg gatatttctt ttacatcttt tcgtaaatca
    13861 tatatttcaa tacaactttc cgaagtaatt ttaaagtctt ttattccaaa ttctttttct
    13921 aacaagtatc cagcaagctc cacattatct acatctactt caacataatc tgtttgatat
    13981 ttgtgtatat ctgacataga aatttcttta ataagtttac catcttgaat aattccaata
    14041 gtgtccgcaa tttgttctac ctctgaaaga atatgactag aaataaatat agttattcca
    14101 taatctgtat ttaacttacg gaataattct cgcatttctc ttatcccctc tgggtcgagt
    14161 gcatttatag gttcatcaag aatcaataat tctggtttag taagaattgc tctagctatt
    14221 gcaagacgtt gtttcatccc taaagaatat tttgaaactt tcttgtttga ttgctttgac
    14281 aatccgacac attccaatac ctctgatata tgttctttgt tataataacc catatattca
    14341 cagtgtaaat caagattctg tttagcagtc aaattctcat ataaatacgg attttcaata
    14401 atagtaccaa tcttttttaa tatttcaaaa tcagtttctg ccactttctt accgaacata
    14461 actatttctc cagaatttgg cttaacaaga tttagcaaca ttttcattac tgtactttta
    14521 cctgccccgt ttggtccaac aaatccataa atttctccct ttttcacatg aatattaaca
    14581 gcattaacaa cagctttatc tttatatatt ttctttaatt gattggtctc tatgatatag
    14641 ttcatcatac tccctccttt ctaaaaacag tatacaaaat aaaattatct tttctctctt
    14701 ttaaatctaa cttttttcta acttttcaat tttactgtaa aaactgtttt tacattaggt
    14761 ctactattta cataaattga acctcccata gagtttacaa gcccttgaga aatagccaaa
    14821 cctaaaccat tgcctttttt tctgtcagca gtataagctc ttgtgaaaat atgctctttt
    14881 tcagtttcag aaatacctga acctttatcc tcaatatcta taaaaacatg tttttccttt
    14941 tcatatagtg aaatccctaa aaatctacca tctgaaccat attttatagc attatcaatt
    15001 aaatttttga gtatacgttg taatgcttct gtgtcaacat ttgcatatat aggtctatct
    15061 tcaatattaa cttcaacatt aaagttttct ttatctagca aatcataaaa ctctaaaata
    15121 atctcatgac ataattgtgt tagattggat ttttgaagat ttaatatcat atctccagat
    15181 tggatttttg ccatattaaa caggttattt attgaattaa caagctcatt tgaatttgtt
    15241 tgcatttttt ccacagttgc tctcattgaa gaagataatt cttctttttg actttgaaga
    15301 tataacattt caatataacc ctttaatact gtgatgggag ttcgtaaatc gtgtgatatg
    15361 tttgtaaata tttgcaacat cgctttttca cttctattat aattaatttg cctgctgtaa
    15421 aacttttcaa gaagtttatt tatatctact gctgtttctt ttacaatcga aatatcagat
    15481 ggtactaaaa tataattaat ttcatcatca tttgttatgt cacgaagtct tgaattgata
    15541 tacttataat cactttccat tttctttgat ttatttcgtt cttttaagta gagagcaaat
    15601 agtacaatcg aaattacaac taaaaataac tccattattc ttcctccatt ttgtagccaa
    15661 ttccccaaag agttttaata taattatttt tttctatacc atcagacaat ttatttctta
    15721 acctgttaat atgagtattt agcacatttt catttccata ataaggttct ttccaaatag
    15781 cgttgtataa ttgttccttt gaaaaagcac gtccttgatt agtcgctagt aatttaacaa
    15841 tttcaaattc tgtatgtgtt aagtttaagt caattccatt ctttgaaact acatattttg
    15901 atatatttat aatcaaatct ttatactgaa taaccatatc ttcagagttt cttgaactgc
    15961 tgtacactgt tgcacgtcta atattagctt taattcttgc aagcaattct acgattgaaa
    16021 aaggctttgt aatataatca tctgcaccaa gattaagccc tagcatttta tctgtgtcac
    16081 tatctttcgc agtagtaata ataacaggaa caacactcac acttcgtata tattttaata
    16141 cttcaaaacc actagacttt ggtaacatca agtccagtaa aataatatca aatgaataat
    16201 ttttaaatat ttcaatagct gtatttccat caatggcagt tttaacagag taattctcac
    16261 taatcagaaa atcgtatacc atttctcgaa tattcttatc atcttcaacc aataagatgt
    16321 ccattattat tcaactcctt ttcgtcagta tacttcaaat gatatattta ttaaaaaggg
    16381 ttgtcaataa ataactgctt atgatattgt atctgcaatt tggtgatact cttctaagtt
    16441 aaagttcttt tcaataagct ttattgaaaa aataattaaa atatagtata ctctcttatc
    16501 tttatctgaa atatacataa ttttctttta ttaacatcta caattaacta ttttatatta
    16561 taaaacacct tttaactctt gctaaaaaat attttataca taaaaaagaa accacattct
    16621 agttttatac agaacatagt ttctttaaaa ataatcaaat ttggatttta aataattaaa
    16681 aacaaacttt tatttgtttt taattttcca attttatagt agataattta gttaattagt
    16741 tcttaaatgt ttatcttctt tagtaaattt ttttcttatt aagaaatatg caactattac
    16801 aacaagttct attgctaaaa atacaccata agtagctata gaatctatgc tatctatgcc
    16861 atttctaaag aaaattaaag caattcctgc tgctataata cctacagcat ttttccctat
    16921 agaattttga ccaaaaccat aaaaagaagg gaaatttcct tttatttcaa tgaatgtcaa
    16981 gattaatcca atagctataa atagtactcc tacaacttcc ataatcatgc taggcatttt
    17041 gacccctcct tattaataat taactactac tataaaaata tttattattt t
//
LOCUS       DAMJYR010000082        17035 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.083, whole genome shotgun sequence.
ACCESSION   DAMJYR010000082 DAMJYR010000000
VERSION     DAMJYR010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 17035)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17035)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17035
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.083"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(129..653)
                     /locus_tag="PC471_002563"
     CDS             complement(129..653)
                     /locus_tag="PC471_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HDF2789681.1"
                     /translation="MENKNPIVTIEMENGKEIKIELYPNIAPNTVKNFVSLVNEGYYN
                     GIIFHRVIPGFMIQGGCPNGTGMGGPGHSIKGEFSGNGFTNNLKHERGVISMARTMAP
                     NSAGSQFFIMHKNSPHLDGQYAGFGRVIEGMDTVDEIASVRTDSADKPQTPQVMKSVT
                     VETFGIDYSDVEKN"
     gene            complement(718..1470)
                     /locus_tag="PC471_002564"
     CDS             complement(718..1470)
                     /locus_tag="PC471_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HDF2789682.1"
                     /translation="MKLGLCLEGGGAKGAYQAGVVKALYDGGINKFYSISGTSIGAIN
                     GYYLYTGNVDNLEKMWTNIKDIQNGNVKIVNNTVDNSPAIDNLRELDDSNIEEMNFYV
                     NYVEVDNKVVSEKIVDVSKMPRNEAIISISYSGLLPSNPNATLGFKEQFVKDVQEGIY
                     DGFKLDGGLIRSALIEPLIGDNVDKIILISTKYNYELPEDIKKVYDEDKIIVVRPNTQ
                     FAPKDTLNFDDEFCKTIYQEGYEIGKNILDRL"
     gene            complement(1501..2799)
                     /locus_tag="PC471_002565"
     CDS             complement(1501..2799)
                     /locus_tag="PC471_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M18 family aminopeptidase"
                     /protein_id="HDF2789683.1"
                     /translation="MDSINFAKNLIDFIYDSPSSFHAVESTKEILDKNGFEELVLNQR
                     WNLRVGGKYYVTKNLSAIVAFVVNSEGIERDGFRIIGSHSDSPTFSIKPNAEIEAEKS
                     YLKLNTECYGGAILSTWLDRPLGIAGRVVLKGDSILKPNERLIDFKKPICIIPNLAIH
                     LNRSINDGHSYNKQKDMLPLVGMLNDTLEKDNFLIKQIASNLDVNMDDIIDFDLFLYE
                     FEKGSLIGANDEFISIGRQDNLAMVHASLNALVNTKGQHGVNVMAVFDNEEVGSSTKQ
                     GADSNMLLNILERICISMGKDREGFFESIYSSFMISSDLAHAIHPNIPEKHDPTNKPI
                     MGKGPVIKINASQAYTSDGYSIAIYKNICREANVEYQEFVNRSDERGGSTIGPISSTH
                     IDIPSVDVGSPILAMHSIRELGNVEDHYSIYKTFSKFFEI"
     gene            complement(2816..5080)
                     /locus_tag="PC471_002566"
     CDS             complement(2816..5080)
                     /locus_tag="PC471_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3553 domain-containing protein"
                     /protein_id="HDF2789684.1"
                     /translation="MNLDTLNPAQREAVEKTEGPVLILAGAGSGKTKVLTTRIAYLIE
                     DKGVQAPNILAITFTNKAANEMRERVEQNIGPETKDMWISTFHSCCVRILRKDINKIG
                     YNRSFVIYDSADQVTLVKDCLKELNLSDKVFEPKAVISAISGAKDKLYTPKQFKDINM
                     ADNRMVKIADIYALYQDRLKRNSALDFDDLILKTVELFKANDEVLAYYRSRFRYIMVD
                     EYQDTSKAQYELIKLLAREHQNICVVGDDDQSIYGWRGADIRNILEFEKDYDNVHVVK
                     LEQNYRSTQVILDAANKVISNNIERKRKKLWSEKKEGELIKIQLTGSEIEEADFIADS
                     IAQIARKENRPYKDFAVLYRANAQARPVEDALNRSQIPYNIYGGTKFYERKEIKDLLA
                     YLRVIQNPQDDISIKRIINVPRRGIGLRTIEKIEDRANLKQESIYSVLIDIETNSDIS
                     TKARASISGFVDIIGTLRTIKEVYPVSKLIEKVLDTTGYMDELVEIRNKNEKDLTGKG
                     EEAQDRIDNLREFISIALEFESSNDDTYENKDLETFLTSIALTSESNDEEDNDRVSLM
                     TIHTSKGLEFPVVFLTGMEEGLFPISRAIKSMSDSQIEEERRLCYVGITRAKEELYMS
                     LTEKRTLYGKTNVAIASRFMEELPEECIERLYKVKKELSYSKASYNMLDKYTKKYMST
                     ISKSKVADKVNATIKDSNKETNPDDIKLGSKVHHPKFGVGTVVSIIGTDVTIAFDQQG
                     IKKINKEYTTLNIL"
     gene            complement(5335..6153)
                     /locus_tag="PC471_002567"
     CDS             complement(5335..6153)
                     /locus_tag="PC471_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HDF2789685.1"
                     /translation="MFKINNLTYQYEKNTNALLNINMDFSKGNVIGIIGSNGSGKSTL
                     FMNLMGILKPTSGEILFKEEKLKYDKRSLYNLRKNVGIVFQDPEKQIFYSKVYDDIAF
                     AMRNIGMDEKTIKERINKALVAVNGIDFIDRPVHFLSYGQKKRVAIASVIAMENEIVL
                     LDEPTAGLDPVSTRSIVDIIKGLNKNNIKIVISSHDMNLMYEICDYIYVLDKGILIDE
                     GKAENVFINENNIIQAGLESPWLVKVHRNMNLPLFKKEEDLYKYWKERELNTNK"
     gene            complement(6146..6844)
                     /gene="cbiQ"
                     /locus_tag="PC471_002568"
     CDS             complement(6146..6844)
                     /gene="cbiQ"
                     /locus_tag="PC471_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt ECF transporter T component CbiQ"
                     /protein_id="HDF2789686.1"
                     /translation="MLIIDKYAYTNRLSKVNPNKKVAIGVIFLIASMVIQNIFILSGI
                     MILMSILVVCVAGIDLKNYLKLLRIPMYFLFLSIGITLVNISFNKADLLYSFEFFSFN
                     VGISKASINMSIHVLFRAMSCLTCVYFCILTTPFNQLIFFFKKLHLPDTFVELSMLIY
                     RFIFIFLEEFSEIYKSQELRFGYINLKTSYKSLGILGSMLYKRLMTRYDDMCISLDIK
                     LYDGKFHIVGDNDV"
     gene            complement(6847..7131)
                     /locus_tag="PC471_002569"
     CDS             complement(6847..7131)
                     /locus_tag="PC471_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     substrate-binding protein"
                     /protein_id="HDF2789687.1"
                     /translation="MSAKTKTKTNILLLLLVVALIIFPLLVNSGAEYGGADGQAESEI
                     TKINPNYKPWFSSPYEPPSGEIESLLFSAQAALGAGVIGYILGLQKGKRS"
     gene            complement(7121..7873)
                     /locus_tag="PC471_002570"
     CDS             complement(7121..7873)
                     /locus_tag="PC471_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006440549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter permease"
                     /protein_id="HDF2789688.1"
                     /translation="MKQNIKLGVIAALMLIILTPVTSNAMHIMEGYLPVKWSIAWGVI
                     FIPFFLFGLKSIGKIVKQDPKKKVLLALCGAFVFVLSALKIPSVTGSCSHPTGVGLGA
                     IMFGPSVMFVLGTIVLIFQALLLAHGGITTLGANAFSMAIIGPIVSFLIFKALKKKDG
                     NNAIPVFLAAAIGDLATYTITSIQLALAFPDPSGGVMASAIKFLGIFFMTQIPIAIAE
                     GILTVIVYNLITENGEKSILENNDKGVKANEC"
     regulatory      complement(8055..8247)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            complement(8379..10151)
                     /locus_tag="PC471_002571"
     CDS             complement(8379..10151)
                     /locus_tag="PC471_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase activation-like protein"
                     /protein_id="HDF2789689.1"
                     /translation="MKWIYDDYEFENRANNLSWTIAGDYNESIDISEKDYSSKEIGLY
                     FAIIAGARRKYVDWDIVKKYLTNRIRKGYNKDVILGLIQVILNTIVEEKVIKDRPGIE
                     DIRRNAYKDIVSRFTKIHNDNIMDKIKYTFVLYSMDKHPALDSMTRRIVNDIRNIDTN
                     QDIMEILKALDVIYLTYFEYILNGEENSLIDENNVESVEVNFDTFADFMYEELYSDEE
                     ENIIESDIDSISSSMLVEGVGDFDDSKCSNSADRVIYVDEDTANKIYSKIEHYYGKTY
                     LSKGEIKKIETQNCRNVHEGCRIHFTDGVLRSECNNVFQLKYVTRQKENNLGKFREHA
                     RMYRQQIKRLKESLSRILIEENAKSRVYSDCGTILANRAWRVGRSNNNKIFYKDIENE
                     KGKYVIDILLDASGSQSRNQGNVAIQAYIIANALTEVGIPNRVMGFSSFLDYTILKRF
                     KDYDDSIKNSENIFEYFCAGNNRDGLAIKSICSGLINRDEENKILIVLSDGRPNDVKI
                     GKSSERTLRGEKAYRGIVGLRDTANEVRKARKQNILVLGVFTGKESELEAERLIYGND
                     FIYSRDITRFSDVVSMYLKKIIRN"
     gene            complement(10165..11094)
                     /locus_tag="PC471_002572"
     CDS             complement(10165..11094)
                     /locus_tag="PC471_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDF2789690.1"
                     /translation="MDIIDNLRVQGVDEKLIEDVLYFRNYYGLEKDLEYRVTKSKTYF
                     YGKDILSMCIAAILEEENILLSGPKATGKNLLADNLGEIFNRPQWNTSFHINTDSSTL
                     IGTDTFIDNEVKLRRGSVYECAINGGFGVFDEINMAKNDAIVVVHSALDYRRIIDVPG
                     YERVNLHPATRFIGTMNYEYAGTKELNEALVSRFMVIDIPPIEEDKLMMILKNEFSDA
                     DEEKLIHFAGIFLDLQLKSQNGEISSKAIDLRGLMASLKTIRRGLKPTLAINMGLTGK
                     TFDVYEKEMVGDVIKTRIPNKWESIDVFPISHI"
     gene            11315..11500
                     /locus_tag="PC471_002573"
     CDS             11315..11500
                     /locus_tag="PC471_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HDF2789691.1"
                     /translation="MKYGCPEVAKKWGISERRVQKLCEESRIPGVSKLGYMWLIPKNA
                     EKPVDGRTKRRKEVQYE"
     gene            11493..12194
                     /locus_tag="PC471_002574"
     CDS             11493..12194
                     /locus_tag="PC471_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2789692.1"
                     /translation="MNSVLIIDDDKELCALMKKCVEQENLSAITAHCGAEGLHLVDKN
                     RHTLSLVILDIMMPDIDGFQVLKKIRETSNIPVIMLTAKNDEDDKVSGLRLGADDYLT
                     KPFSINELMARVNSLIRRYTMLNPAFITDTDCIIINGMIIDKTNRIVSVNNIPVQLTS
                     KEFDLLAFLASNKGRVFTKKQIYTQVWEEEYAFDDSNIMSFISKLRKKIEPDPNHPFY
                     ILTVRGVGYRFNKEA"
     gene            12196..13119
                     /locus_tag="PC471_002575"
     CDS             12196..13119
                     /locus_tag="PC471_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF2789693.1"
                     /translation="MNTSVYLLLILVLSLIIIAYLVSVLLRVRIQLTLIKDALEDIKN
                     GNLNRRILTDENDITKQICYDINEIAINSQFQLIQQKQSEQAYKQLMTSLSHDVKTPL
                     ASLVGYLEAVESKIVVGEEKDEYIHVASNKAHYLKNFVENLFEWVKLDSKEQVFHFDI
                     FDLNELSRNIISDWIPVLESSHFEYEFDIPEIEYFLRIDANAYTRIINNLLQNIITHS
                     SGNKMTLRIFENKEQAQIIITDNGKGISSDNLPHIFERLYQCDHSRASKGNGLGLAIA
                     KELINAHKGNITANSTPGMGTEFTILLPKAL"
     gene            13276..14199
                     /locus_tag="PC471_002576"
     CDS             13276..14199
                     /locus_tag="PC471_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019639192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2789694.1"
                     /translation="MNDFVIETKQLTKIYGNQTVVDKVNLHAKKGRIYGLLGRNGAGK
                     TTIMKMILGLTSITYGEVDVFGENIKGREKQVYPRIGAIIETPGFYPNLTGTENLEIF
                     AKLRGTAAPNSVKRALEIVGLPYKNKKLFSEYSLGMKQRLGIANAILHDPELLVLDEP
                     TNGLDPIGIAEMRKFIRDLSVEHGKTILISSHILSEISLLADDIGIIDHGVLLEESSM
                     SELEKKNSKYILLQVSDIPKTSLILERQFNTKDYSVQDDYTLHLYNTNLDMGEINKAL
                     VMQNVTVISSQLCNDTLEDYFKKITGGEGIA"
     gene            14192..14929
                     /locus_tag="PC471_002577"
     CDS             14192..14929
                     /locus_tag="PC471_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2789695.1"
                     /translation="MLKLIQTEFLKLRRRKLIWLMLLSALIMPFFALLYFNYFGKTGV
                     EPIKFYKWSAFGYTLWIILPVVLGIFCTMLIHEENQYDMLKQLWIVPISKMGYFFSKF
                     FVVLIYSICFMLITAVASVLFSVLSGYVVFSWASILYLLKKCLEIGVITAFVMLPILA
                     IATSQKGYILPICITLVYAFSGFVIMTINMYLHPLSSMGVIIMRNKDIPGLIFSQEIN
                     VPLAFLCICIWSIVSVLFANTALGRRK"
     gene            14940..15713
                     /locus_tag="PC471_002578"
     CDS             14940..15713
                     /locus_tag="PC471_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2789696.1"
                     /translation="MWKLLWAECQKIRRSKIVWITVFASIMIAVIVFAGGQDVYNDPD
                     LHYGLKSVYDGSRYIDNAGWYMDAVQPWSTFFVLPAVIALLGSYIICREEEEDTIKSL
                     RLIPINETKLTIAKMIITFIFSILLYLLLFTITFLTESVLHFSDLSTKLVLSCMKEYF
                     LDGIGVFFAISPIIALVSRMKKGYWLALVFTEVYSIAGLFAGMSNVLQTFFPIAAIFN
                     ISGYYITTGEKIMGSIISLLLCACLSAFILKGLKHNEKN"
     gene            complement(16254..16862)
                     /locus_tag="PC471_002579"
     CDS             complement(16254..16862)
                     /locus_tag="PC471_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HDF2789697.1"
                     /translation="MSVINSFILQYGLISVFVLIMIEYACFPLPSEVVLPLCGAIAAR
                     NHFGFLTILILSIIAGILGSIFCYTVGNWGGKSIINKIIEICPKAKKGIFASQDYFNK
                     YSSISVCVCRLIPLCRTYISFIAGIAGQNIITFVISSIVGITIWNTSLISIGYIFSES
                     WVRIMSHYNDYKFLVLIIPISIIFIGFVIKSFIHKKHHKIRL"
BASE COUNT         5491 a         2951 c         2154 g         6439 t
ORIGIN      
        1 aatacaaata aattaaaaag tgaaatgtat ttgtctaaaa acataataac atttcacttt
       61 atcctcattt ttaagtaata tatattttta atttttattt ataactaaat ctagttttta
      121 tataaatttt agtttttttc tacatctgag taatctattc caaaagtttc tacagtaaca
      181 gatttcataa cttgtggagt ttgtggctta tcagctgaat ctgttctaac tgatgctatt
      241 tcatcaactg tatccatacc ttctatcact ctaccaaaac ctgcatactg accatctaag
      301 tgtggtgagt ttttatgcat tatgaaaaat tgagaaccag ctgaattagg tgccatagtt
      361 ctagccattg atatgactcc tctttcatgt tttaaattgt tagtaaatcc attaccacta
      421 aattcacctt ttattgagtg acctggacca cccattcctg taccatttgg gcatcctcct
      481 tgtatcataa atcctggtat tactctatga aatattattc cattgtaata tccttcattt
      541 actaatgata caaagttttt aactgtattt ggagctatat ttggatataa ctcaattttg
      601 atttcttttc cattttccat ttctatagtt actataggat ttttattttc cataatttat
      661 taccttcctt ttcattagtt aaattacata attacattta ttatattata ctctaaatta
      721 aagtctatca agtatatttt ttcctatttc atatccttct tgataaatag ttttacaaaa
      781 ctcatcatca aagttaagcg tgtctttagg agcaaattga gtattaggtc taactactat
      841 tattttatct tcatcatata cttttttaat atcttctggc aactcataat tatattttgt
      901 ggatattaaa ataattttat ccacattatc cccaattaat ggttcaatta atgcactcct
      961 aattaaacct ccatctaatt tgaaaccatc ataaattcct tcttgcacat ctttcacaaa
     1021 ttgttcttta aatccaagag ttgcgtttgg atttgatggt aacaacccac tgtatgaaat
     1081 gctgataatg gcttcattac gaggcatttt tgatacgtca acaatctttt ctgatactac
     1141 tttattatca acttcaacat aattaacata aaagttcatt tcttctatat tactatcatc
     1201 tagttccctt aggttgtcta tagcaggaga attatccaca gtattattta ctatttttac
     1261 atttccattc tggatatctt ttatatttgt ccacattttc tccaagttat caacattccc
     1321 tgtgtacaaa taatatccat ttattgctcc tattgatgtc cctgaaatcg aataaaattt
     1381 atttattcct ccatcataca aggctttaac tactcctgct tgataagccc cttttgctcc
     1441 tccaccttct aaacataatc ctaatttcat agtctcatct ccaaattgtt tatcttatat
     1501 ttatatttca aaaaacttac taaatgtttt atatatacta tagtggtcct caacatttcc
     1561 taattctctt atagaatgca ttgctaaaat tggacttccc acatccacag atggtatatc
     1621 tatatgagta gaagatattg gacctatagt acttcctcct ctttcatctg acctattaac
     1681 aaattcttga tactcaacat tagcttctct acatatattt ttataaatag ctatagaata
     1741 tccatcacta gtatatgcct gactagcatt tatctttata accggacctt ttcccattat
     1801 tggtttatta gttgggtcat gcttttctgg aatgtttgga tgaatagcat gagctaaatc
     1861 tgatgatatc ataaaagatg aataaataga ctcaaagaat ccctctctat ccttacccat
     1921 tgatatgcaa attctctcca atatatttaa caacatgttt gaatctgcac cttgtttagt
     1981 agagctacca acttcttcat tgtcaaatac agccattaca tttacaccat gttgaccttt
     2041 agtattaaca agagcattca aactagcatg taccattgca agattatctt gtctcccaat
     2101 agatataaat tcatcattag cccctattaa acttcctttt tcaaattcat ataaaaataa
     2161 atcaaaatct ataatatcgt ccatatttac atctaagtta gatgctattt gctttataag
     2221 aaaattatct ttttctaatg tatcatttaa catcccaact aaaggtaaca tatctttttg
     2281 cttattatat gagtgaccat catttatact tctatttaaa tgtattgcta gatttggtat
     2341 tatacaaatt ggctttttaa agtctataag cctttcattt ggttttaata tactatcacc
     2401 ttttaaaact actctaccag ctatcccaag aggtctatcc aaccatgtac ttaatattgc
     2461 tcctccatag cattctgtat ttaacttcaa ataagatttt tcagcttcta tttctgcatt
     2521 tggtttaata ctaaatgtag gagaatctga atgagagcct attattctaa atccatctct
     2581 ttctatacct tctgaattta ctacaaaagc tactatagct gataaattct tagtaacata
     2641 atactttcca ccaactctta gattccatct ttgattcaaa actaattctt caaatccatt
     2701 tttatctaag atttccttag tactctcgac tgcatgaaaa gacgacggac tatcatatat
     2761 gaagtctatt aagtttttag caaagtttat actatccatt cctcatttac ctccattata
     2821 aaatatttag tgttgtatat tctttattta tttttttaat tccctgttgg tcaaaagcta
     2881 tagtaacatc tgtaccaatt atagaaacca ctgtcccaac cccaaattta ggatgatgta
     2941 ccttagaacc aagttttata tcatctggat tagtttcttt attagaatct tttattgtag
     3001 cattaacttt atctgcaact ttagatttgc ttatagtact catatacttt ttagtatatt
     3061 tatctaacat attataactt gctttagaat agcttagttc tttttttact ttgtacaatc
     3121 tttctataca ttcttctggc agttcttcca taaatcttga agctattgca acatttgttt
     3181 taccatataa tgttctcttc tctgtcaatg acatatatag ctcttcttta gctctagtta
     3241 taccaacata acaaagtctt ctctcttctt ctatttgtga atcactcata gattttatag
     3301 ctcttgatat aggaaataac ccttcttcca tacctgttaa aaacacaaca gggaactcta
     3361 aacctttgga tgtatgaatt gtcataagcg aaaccctatc attgtcttct tcatcatttg
     3421 attcagatgt aagtgctata cttgttagga atgtctctaa gtctttattt tcataagtat
     3481 catcattact actttcaaac tccaaagcta tagaaataaa ttctctcaaa ttatcaattc
     3541 tgtcttgagc ttcttcacct ttgcctgtta aatctttttc atttttattt cttatctcta
     3601 caagctcgtc catatatcct gtagtatcca agactttttc tataagtttg ctaacaggat
     3661 aaacttcttt tatagttcta agagtcccta taatatcaac aaatccactt atacttgccc
     3721 ttgccttagt agatatatct gaatttgttt ctatatcaat taatactgag tatatacttt
     3781 cctgttttaa gttagctcta tcttcaattt tttctatagt tctaagccct atacctcttc
     3841 taggaacatt tataatcctt tttatagata tatcatcttg tggattttga ataactctta
     3901 aatacgccaa caaatccttt atttcttttc tctcatagaa ttttgtacca ccatatatat
     3961 tgtatggaat ttgacttcta tttaaagcgt cctctacagg tcttgcctgt gcatttgctc
     4021 tataaagtac tgcaaaatcc ttataaggtc tattttcttt tctagctatt tgtgcaattg
     4081 aatcagcaat gaaatcagcc tcttctattt cactaccagt taattgtatc tttataagtt
     4141 ctccttcttt tttttcactc caaagctttt ttctttttct ttctatatta ttagatataa
     4201 ctttattagc tgcatctaat ataacttgtg tagacctata attttgttcc aactttacaa
     4261 catgtacatt gtcataatct ttctcaaatt caagtatgtt tcttatatct gcacctctcc
     4321 atccataaat actttggtca tcatctccaa caacacaaat attttgatgt tctcttgcta
     4381 ataattttat caattcatat tgagctttac ttgtatcttg gtactcatct accattatgt
     4441 atctaaatct acttctgtaa tatgctaaca cttcatcatt agccttgaaa agttctacag
     4501 ttttaagtat taaatcatca aagtcaagtg cactatttct ttttaaacgg tcttgatata
     4561 gcgcataaat atctgcaatc ttaaccattc tattatctgc catattgata tctttaaact
     4621 gctttggagt ataaagtttg tcttttgctc ctgaaattgc acttataact gcttttggtt
     4681 caaacacttt atcacttaaa tttaattctt ttagacagtc tttaaccaat gtaacttggt
     4741 ctgctgaatc ataaattaca aaacttctat tgtaaccaat cttatttata tcttttctta
     4801 gtatacgaac acagcaagaa tggaatgtac ttatccacat atccttagtt tcaggaccta
     4861 tattttgttc aactctctct ctcatttcat ttgcagcttt gtttgtaaat gttatagcta
     4921 atatatttgg tgcttgtaca cctttatctt ctataagata tgctattctt gttgttaata
     4981 ccttagtttt acctgaacca gcaccagcca gtataagtac tggaccttca gtcttttcta
     5041 ctgcttctct ttgagcagga tttaatgtat ctaaattcat atttctcctc ctgttaacag
     5101 ccataaatat attttaatct aaaatatgaa ttacttcaaa ttataattat gtattatatt
     5161 aatagattat ttccaatata ttacaattaa ataacatttt tccatataat ttatttcatt
     5221 gtttaataaa aaaaataacc ttatgatttg gggaatcata aggcaccttt aaggggtcta
     5281 ggttaagggt tattatatat aaatctattt ttttaaaata ggtttagggc ttaattattt
     5341 atttgtatta agttctcttt ccttccaata cttatacaaa tcttcttctt tcttaaataa
     5401 gggtaaattc atatttctat gtacttttac taaccatggg gattctaggc cagcttgtat
     5461 aatattattt tcatttataa acacattttc tgcttttcct tcatcaatca aaatcccctt
     5521 atccaataca tatatgtaat cacatatttc atacattaaa ttcatatcat gactggatat
     5581 tactattttt atattatttt tatttaatcc ctttattata tctacaatag acctagtact
     5641 tactgggtct aaaccagctg taggttcatc aagtaataca atttcatttt ccattgctat
     5701 aactgatgct attgccactc tttttttctg tccatagcta agaaaatgta ctggcctgtc
     5761 tataaaatct attccattaa cagctactag agctttgttt attctctctt taattgtttt
     5821 ttcatccata ccaatgtttc tcattgcaaa ggctatatca tcatatactt ttgaataaaa
     5881 aatttgtttt tctgggtctt ggaagacaat acctacattt tttctaaggt tgtataaact
     5941 tctcttatca tattttaact tttcttcttt aaaaagaatt tctccagatg taggctttaa
     6001 tatacccatt aaattcataa ataatgttga ttttcctgaa ccatttgaac ctattatacc
     6061 tataacattt ccctttgaaa aatccatatt aatgtttaat agtgcattgg tatttttttc
     6121 atattgatac gttaaattat ttattttaaa catcattatc ccctactatg tgaaatttac
     6181 catcatacaa ttttatgtct aatgatatac acatatcatc ataccttgtc ataagtcttt
     6241 tgtacagcat acttccaaga atacctaagg atttatatga agtttttaaa tttatatatc
     6301 caaatcttag ctcttgtgac ttgtatattt ctgaaaattc ttctaaaaat atgaatataa
     6361 acctatatat taacattgat aactcgacaa aagtgtcagg taaatgaagt tttttaaaaa
     6421 agaatattaa ttgattaaaa ggtgttgtaa gtatacaaaa atacacacaa gtcaaacaag
     6481 acatcgctct aaataacaca tgaatagaca tgttaataga cgcctttgat attcctacat
     6541 taaaactaaa aaattcaaaa ctatataata aatctgcttt attaaaagat atatttacaa
     6601 gagttattcc tatactaaga aataaaaagt acattggtat tctaagtaat tttaaataat
     6661 tttttaaatc tattccagcc acacaaacaa ccaaaatact cattaaaatc ataattccac
     6721 ttaaaataaa tatattttgt attaccatag aagcaatcag aaaaattact cctatggcta
     6781 cttttttgtt tggatttact ttactcaatc tatttgtata tgcatattta tcaattataa
     6841 gcatgattaa cttctctttc ctttttgaag acctaatatg tatcctatta caccagcccc
     6901 taaagctgct tgtgcagaga ataataaact ttctatttct ccacttggtg gctcatatgg
     6961 actgctaaac catggtttgt aatttggatt tatcttagta atttcacttt cagcttgacc
     7021 atctgctcct ccatattcag cacctgagtt gacaagtaat gggaatataa taagagctac
     7081 tactaataaa agtaataata tgttagtttt tgtttttgtt ttagcactca ttagctttaa
     7141 ctcctttatc attattttct agtatgcttt tttctccgtt ttctgtaatt aaattatata
     7201 caattacagt aagtatacct tctgctattg ctattggtat ttgagtcata aagaaaattc
     7261 ctaaaaattt aattgctgaa gccattaccc cacctgatgg gtctgggaaa gccagtgcta
     7321 attgtattga agttattgta tatgtggcta agtctcctat tgctgctgca agaaatactg
     7381 gaatagcatt atttccatct tttttcttta aagccttaaa tattaggaat gatacgattg
     7441 gccctattat cgccattgaa aaagcattag ctcctaaagt agttattcct ccatgtgcta
     7501 ataacaatgc ttggaatatt aaaactatag ttccaagaac aaacattacg cttggtccaa
     7561 acattattgc tccaagacct acaccagtag gatgtgaaca acttcctgtt actgaaggta
     7621 tttttagtgc tgataataca aacacaaatg ctccacataa agctaataat actttctttt
     7681 tagggtcttg ctttactatt ttacctattg attttaatcc gaataagaaa aatggtataa
     7741 atataactcc ccatgctata gaccatttta ctggtaagta accttccatt atatgcattg
     7801 catttgatgt aactggagtt aaaatgatta acataagtgc agctataacc cctaatttaa
     7861 tattctgttt catcttagta tccctctctc cctttctaat aattaaaaca taataataca
     7921 tgtctgattt atttaataaa ataaaaaagc ctaactcgat ggttaggctt ctaattttcc
     7981 acaatctaaa tacaaacaca taaaacaaac atatctgtat aattattata agaaactttc
     8041 tccatccccg aagaaattac tttaatattt taggcaggtc tcctgactta gcttcatcct
     8101 actccttttc cttcccatga gatataaaaa atatcatcat agtggctaac aaaagtttcg
     8161 tctgcatcac agtagcgggg gctgtaaagg aatttaacct ttttccctat taatcttgct
     8221 taaagaacct ataatattat atatttaatt tttaaattat tcttaaatca ttttctatat
     8281 aaatcattgt ataccttttc tgtgaaaatt tctacatttt ttataaattt tattcatttt
     8341 tatattttta tgtatataaa ttcttttttg taagtttatt aatttctaat tatcttcttt
     8401 aaatacattg aaactacatc agaaaatcta gttatatctc ttgaatatat aaaatcattt
     8461 ccatatatta atctttcagc ttcaagttct gattcttttc ctgtaaatac tcctaatact
     8521 aatatatttt gctttctagc ttttctcact tcattggctg tatctctcaa accaactata
     8581 cccctataag ccttttctcc tcttaatgtt ctctcactac tttttcctat ctttacatca
     8641 tttggtcttc catcgctgag aactatcagg attttatttt cttcatctct atttattaga
     8701 ccactacata tagattttat tgccaaacca tctctattat taccagcaca aaaatattca
     8761 aatatatttt cactattttt tatactatca tcataatctt taaatctttt taatattgta
     8821 tagtctaaaa aactagaaaa tcccatcact ctatttggta tgccaacttc tgttagagca
     8881 tttgcaatta tgtaagcttg aattgccaca tttccctgat ttctactttg agagccactt
     8941 gcatccaata atatatcaat aacgtatttc cctttttcat tttctatatc tttataaaat
     9001 attttattat tattacttct accaactctc caagctctat tggctaatat agtaccacaa
     9061 tcagaatata ctctactttt agcattttct tctattaaaa ttcttgacag actttctttt
     9121 aatctcttta tttgctgtct atacattcta gcatgttctc taaattttcc taaattattt
     9181 tctttttgtc ttgttacata ttttagttga aatacattat tacactcact tctaagaact
     9241 ccatctgtaa agtgtattct acaaccttca tgaacatttc tacaattttg tgtctcaatt
     9301 tttttaattt cacctttact caaataagtt tttccataat aatgttctat ttttgaatat
     9361 atcttatttg cagtgtcttc atcaacatat ataactcggt ctgctgaatt gctacattta
     9421 gaatcatcaa aatcccctac accttctaca agcatggatg aagatattga atctatatca
     9481 ctttcaataa tattttcttc ttcatcacta taaagttctt catacatgaa atcagcaaat
     9541 gtatcaaaat ttacttctac actttctaca ttattttcat caataagact attttcttct
     9601 ccatttaata tgtattcaaa ataagttaaa tatataacat ctaatgcttt taaaatctcc
     9661 ataatatctt gattggtatc tatatttctt atatcattaa ctattcttct agtcatacta
     9721 tctaatgctg ggtgcttgtc catactataa agtacaaatg tgtatttaat tttatccata
     9781 atattatcat tgtgtatttt ggtaaaacgt gaaacaatat ctttataagc attccttctt
     9841 atgtcttcta tccctggcct gtctttaatg accttttctt caactattgt atttaatata
     9901 acttgaatta gacctagtat aacatctttg ttgtatcctt ttcttattct atttgtaaga
     9961 tattttttaa ctatatccca atctacatac tttcttctag cacctgcaat tattgcgaaa
    10021 tatagaccta tttcttttga agagtaatct ttttctgata tatctatact ttcattgtaa
    10081 tcaccagcta ttgtccatga taggttatta gctctatttt caaattcata atcatcatat
    10141 atccatttca taatctcacc tatattaaat atgacttatt ggaaacacat ctatactctc
    10201 ccatttatta ggtattctag ttttaattac atctccaacc atttctttct catatacatc
    10261 aaaagtcttt cctgtaagtc ccatattaat tgcaagagtt ggttttaatc ctcttcttat
    10321 agtcttaaga gatgccataa gaccacgtaa atcaatagct ttactagata tctctccatt
    10381 ttgagacttc aattgtaaat ctaaaaatat tcctgcaaag tgtattaatt tttcttcatc
    10441 agcatctgaa aattcatttt ttaaaatcat cattaactta tcttcttcta taggaggaat
    10501 atctattacc ataaatctag ataccagtgc ttcattaagt tcttttgttc ctgcatattc
    10561 ataattcata gtcccaataa atcttgttgc tggatgtaga ttgactctct catacccagg
    10621 tacatcaatt attcttctat agtctaaagc tgaatgtacc acaactatag catcattttt
    10681 tgccatatta atttcatcaa acaccccaaa acctccatta atagcacact cataaactga
    10741 gcctcttcta agtttaactt cattgtctat aaatgtatct gtacctataa gtgttgaact
    10801 gtctgtattt atatgaaatg atgtattcca ctgaggtcta ttaaatattt cccctagatt
    10861 atcagcaagt aagttttttc ctgttgcctt tggtcccgat aataatatat tttcttcttc
    10921 taaaatagct gctatacaca tagacaagat atctttacca taaaaataag ttttagactt
    10981 tgttactctg tattctaaat ctttttctaa accataataa tttctaaaat acagtacatc
    11041 ttcaataagt ttttcatcta ccccttgcac ccttagatta tctattatat ccatattttt
    11101 acccctttta tataaatagt atttgtttca tttcttatca ccattttagc ataaaaggat
    11161 ataagctaaa attgattttt taatctcccc attttaagta aaaccatgca ccattttatc
    11221 actacaaaat taagagtact acttactgat atttcttgat tttgttcgtc tatacgtata
    11281 taatatatgc tgataaatta tgaggaaagg atgattgaaa tatggatgcc cagaagtagc
    11341 aaaaaaatgg ggtatttctg aaagaagagt acaaaaacta tgtgaagaaa gtcgcatacc
    11401 aggagtttca aaactcgggt atatgtggct gataccaaag aacgcagaaa agcctgttga
    11461 tggtagaaca aaaagaagaa aggaagtaca atatgaatag cgttttgatt atagatgatg
    11521 acaaagaact ttgtgctctt atgaaaaaat gtgttgagca agaaaattta tctgctatta
    11581 ctgcacattg tggtgcagaa ggtctacatt tggtcgataa aaatagacac acactctctc
    11641 ttgtaatttt ggacataatg atgccagata tagacggttt tcaagttctg aaaaaaatta
    11701 gagaaacaag taatattcca gtaattatgc ttactgctaa aaatgacgag gatgataaag
    11761 tttccggact acgtttaggt gctgacgatt acctaacaaa accgttcagc ataaacgaac
    11821 ttatggctcg tgtcaattct cttattcgtc ggtatactat gttaaaccct gcttttataa
    11881 cagacacaga ttgcataata ataaatggca tgattattga caagacaaac cgaattgttt
    11941 ctgtcaataa tattcctgtt caacttacaa gcaaagaatt tgatttactt gcttttctgg
    12001 cttctaataa gggacgggtg tttaccaaaa aacagattta tacacaagta tgggaagaag
    12061 aatatgcttt tgacgatagt aacatcatgt ctttcatcag taaattgaga aaaaagattg
    12121 aaccagaccc caaccatcca ttttatattt tgacagttcg tggtgtcggt tatcgtttta
    12181 ataaggaggc ttaacatgaa tacaagtgtc tatctgcttt tgatattggt tcttagcctg
    12241 attattatag cgtaccttgt ttctgtcctg ctacgtgtcc gaatacaact tacacttata
    12301 aaagatgctt tagaggatat aaaaaatgga aaccttaatc gacgtatttt aacagatgaa
    12361 aacgacatta caaaacaaat ttgctatgat attaacgaaa tagcaatcaa tagccaattt
    12421 caacttattc agcaaaaaca atcagaacaa gcctacaaac agctaatgac aagtctctcc
    12481 catgatgtaa aaactccact tgcttctttg gtcggttatt tggaagcagt agaaagtaaa
    12541 attgttgtag gagaggaaaa agacgagtat atccatgttg cgtctaataa ggctcattac
    12601 ttaaagaatt ttgtagaaaa tctatttgaa tgggtaaaat tggattctaa ggaacaagtt
    12661 tttcattttg atatttttga cttgaatgaa ttatcccgta atattatatc tgattggata
    12721 cctgttttag aaagcagtca ttttgaatat gaatttgata taccagaaat agagtacttc
    12781 ttacgtatag acgcaaatgc atatacccgt atcattaata acctattaca aaatattatt
    12841 acccatagta gtggcaacaa aatgacattg agaatttttg aaaacaaaga gcaagctcaa
    12901 attataataa cagataatgg gaaaggtata tcttcagaca atctcccaca tatttttgaa
    12961 aggttgtacc aatgcgacca ttcacgagct tcaaagggaa atggtttagg cttggcgatt
    13021 gcaaaggaac ttattaacgc tcataaggga aatattaccg ccaacagcac tcccggtatg
    13081 ggaacagaat ttacaatatt gcttcctaaa gccctgtaaa agtacgaggc tttcttactc
    13141 taaaacaaga taaaagcaag attatggcaa ggttttggca aggttcatgt tttataattc
    13201 taattgtagt tgattttgac gttgtatcaa ctatcttttg tagaaacaaa tttattaaaa
    13261 gtgaggaaat tatatatgaa tgattttgtg attgaaacaa agcaacttac aaaaatttat
    13321 gggaatcaaa cagtagttga caaagtcaat ctccatgcaa aaaaaggccg aatatatggt
    13381 ctgttaggac gcaacggagc tgggaaaacc acaattatga aaatgatttt aggattgact
    13441 tcaatcacgt atggcgaggt agatgtattt ggagagaata ttaagggacg tgaaaaacaa
    13501 gtatatcccc gtatcggtgc aattatcgaa acgccaggat tttatcctaa tctgacagga
    13561 acggagaact tggagatttt tgcaaaactg cgtggtacag ccgcccctaa ttctgtaaaa
    13621 agagcattgg aaattgtagg cttaccttat aaaaataaaa agctgtttag tgaatattca
    13681 cttggcatga aacagcgact tggtattgcc aacgctatcc tgcatgaccc agaactgtta
    13741 gttttagatg agccaaccaa tggtcttgac cctattggta ttgcggagat gagaaagttt
    13801 attagggact tgagcgttga gcatggaaag acaattctta tttccagtca tattctttca
    13861 gaaatctcat tgttggctga tgatattgga attatcgacc acggtgttct tttggaagaa
    13921 agtagtatga gtgaactaga aaagaaaaac agtaaatata tcctgctaca agtttcagat
    13981 attccgaaga catcacttat tttagagcgt caatttaaca cgaaggatta ttccgttcaa
    14041 gatgactaca cgttacacct ttataacact aatctggata tgggtgaaat caataaagca
    14101 cttgtaatgc agaatgtaac tgttattagt tctcaacttt gtaatgatac tttagaggac
    14161 tactttaaaa aaatcacagg aggagagggc attgcttaaa cttattcaaa cagaatttct
    14221 taaactgcgt cgtaggaaac ttatttggct gatgttgttg tctgctctca ttatgccgtt
    14281 tttcgcacta ctatacttca attattttgg taaaacaggt gttgagccaa taaagtttta
    14341 taaatggtct gcatttggtt atacactttg gattattttg cctgttgttt tgggtatatt
    14401 ttgcaccatg cttatacacg aagaaaacca gtatgatatg ctcaaacagc tttggattgt
    14461 acctattagt aaaatgggat acttttttag taaatttttc gttgttttga tttactctat
    14521 ttgttttatg ctgattacag ccgttgcttc tgtgttattt agtgtacttt ctggatatgt
    14581 cgtatttagc tgggcaagca ttttatattt gttgaaaaaa tgtttagaaa ttggtgtaat
    14641 cactgctttt gtaatgttgc cgattttggc aatcgctaca tctcaaaaag gatacattct
    14701 cccaatttgt atcacattgg tatatgcctt ttccggtttt gttataatga caataaatat
    14761 gtatttacac ccattatcca gtatgggagt tattatcatg agaaataaag acattccagg
    14821 tcttatattt tcacaggaaa ttaatgtccc attagcattt ctttgtattt gtatttggag
    14881 tattgtatca gtattgtttg caaacactgc actaggaaga agaaagtgag gtgtagataa
    14941 tgtggaaatt actttgggct gaatgtcaaa aaattcgtcg ttcaaaaatt gtttggatta
    15001 cagtttttgc atccattatg atagcagtca tagtttttgc aggagggcag gatgtatata
    15061 atgacccaga tttacattat ggcttaaaga gcgtttatga cggttcacgg tatatagaca
    15121 atgcaggttg gtatatggac gcagtacaac cttggtcaac tttttttgtt cttcctgctg
    15181 tcattgccct attaggaagc tatattattt gtcgtgaaga agaggaggac actattaaat
    15241 ccctgcgtct tattcccata aatgaaacaa aattaaccat agcaaaaatg attattactt
    15301 ttatattcag tatacttctt tatttgttac tctttactat aacatttttg actgaatccg
    15361 ttttacattt ttccgattta tcgacaaaac tggttttaag ttgcatgaaa gagtattttc
    15421 ttgatggcat aggtgtattc tttgcaattt ctcccattat tgccttggta tcgcgcatga
    15481 aaaaaggata ttggcttgca ttagtattca cagaagttta ttctattgct ggactatttg
    15541 cgggtatgtc aaatgtctta caaacctttt ttcctattgc ggcaatattt aatatttctg
    15601 gatactatat aactactgga gaaaaaatta tggggagtat cataagttta ttactttgtg
    15661 cctgcctttc tgcatttata cttaaagggc taaaacacaa tgaaaaaaat tgaaataggt
    15721 atatcaaaat gaaaaagata aaaaaactga ttattctaat tgtattcttg tttctttttg
    15781 gcatgttaca ctgttatact gaatggaaat tcaacaataa cccacctaaa ggggtaatag
    15841 aaagaataaa tgaatggact gataagattg gactggatta gtaacttatg ttaattatag
    15901 tgaatgtaat attagcaagt tattgtttcg tattttaata ctataacttt aaatatttgg
    15961 ttttaatctt ccaaacgaat ataaaaaaac tgtcacagag gcaacatatt tttgaatatt
    16021 tataaagtgg tagttttgat tttcaaagct gccgcctttt cttctatata agcaaactta
    16081 tcctcaacaa agtgtaaaac atggtacatt ttaagaaaaa agtaccacag acacaagcaa
    16141 taaaagaaac gccgagaacc tacaaaaacg tagtgcttat gcagatttac gacgccttct
    16201 attttctcaa ccgtcttaat gaccttgtac aaaagaaatt tttctaatat tttttataat
    16261 cttatcttgt gatgtttttt atgtataaat gacttaataa caaatccaat aaaaattatg
    16321 gatattggta taattagaac gaggaattta taatcattat aatgtgacat tattcttacc
    16381 caactctcag aaaaaatata tcctatactg attaatgaag tattccatat ggttattccc
    16441 acgatagatg atattacaaa agttataata ttttgacctg ctatacctgc tataaaagat
    16501 atgtatgttc tacataaagg gattaatcta catacacaaa cagaaataga agagtatttg
    16561 ttaaaataat cttgagatgc aaatattcct ttctttgcct ttggacatat ctctattatt
    16621 ttatttatga tagatttacc tccccaattt cccactgtat aacaaaatat cgaaccaagt
    16681 atacctgcaa ttatacttaa aattaaaatt gtcagaaatc caaaatgatt tcttgcagca
    16741 attgctccac ataaaggtaa aacaacttcg ctaggtaatg gaaaacaagc atactcaatc
    16801 ataattaata caaatacaga gattaatcca tattgtaaaa taaaactatt gattacagac
    16861 acttacttat ctatctcctt tcttatttaa aggttttaag ggtacatgtt ctaaggattc
    16921 tcaccctata tttaaagaat caatctcttt aaactattat atgtatttac acaaagcatt
    16981 tataacatac tttaaaaata gtaccttaaa ataagaaact taataaataa aaaaa
//
LOCUS       DAMJYR010000083        16809 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.084, whole genome shotgun sequence.
ACCESSION   DAMJYR010000083 DAMJYR010000000
VERSION     DAMJYR010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 16809)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16809
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.084"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            280..738
                     /locus_tag="PC471_002580"
     CDS             280..738
                     /locus_tag="PC471_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDF2789698.1"
                     /translation="MEFINFVSDKVIDLKMDCKTKEDFFKEIHNKVFDLGFVKEEFGE
                     KILARENVFPTGLNLGDYGVAIPHTDAEYIKEQFISVCTFNEPVVFSSMEDQDEKVPV
                     NLAFVLGLNQPHSQLSVLTELMGIMQNKELVDKLMSSTDKEEVLKTLKSL"
     gene            799..1074
                     /locus_tag="PC471_002581"
     CDS             799..1074
                     /locus_tag="PC471_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDF2789699.1"
                     /translation="MKKILVACGAGIATSTVVCDKVERLVKENNIQAEVIQCKIAECA
                     TKQEGADLIVSTTILPTTYNIPAIKATAYITGVNTAALDKKILDALK"
     gene            1133..2407
                     /locus_tag="PC471_002582"
     CDS             1133..2407
                     /locus_tag="PC471_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012951111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HDF2789700.1"
                     /translation="MDKLLAVVQYILNMGPTVILPITIVVLGMIFRIKFTKALKSGLT
                     IGIGFVGINLVVNLLTSTLGPAAQGMVERFGLNLTVIDVGWPTTASATWASPVAPILI
                     PVCLLVNLLLLYFNKTKILNVDIWNYWHFIVAGAVGYIVTGSYVWAIFCAILMELLVL
                     FIAEKTAKNVQEFYDLEGIALPTGSTASFAPLGYLVGKFVEKIPGINKIQADPDSIQK
                     RFGIFGEPMMMGIILGIILGALAGYDVAGIFNVGISMGAVMLLMPRMVRILMEGLIPI
                     SESIRDFLQAKYGDRELYIGLDAAVATGHPAVISTALILVPITLFLAVILPGNKVLPF
                     GDLATIPFYMAFIVCFRKGNIVQSVITGTIVIAISLYMATNFADVHTQLLQQANMMPE
                     GTTMVTSIDTGGNLLKWGILKLSQLVSSFGIF"
     gene            2537..3589
                     /locus_tag="PC471_002583"
     CDS             2537..3589
                     /locus_tag="PC471_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HDF2789701.1"
                     /translation="MKAAVLHGTNDMRFEDIEIKPCESDEVKIKVMAAGICGSDPPRV
                     LKHWKYPVPAIPGHEFSGVIAEVGKDVKNVKVGDRVVAIPFIPCNECEYCKRGLFSLC
                     DDHGMLGAKSFGAFAEYVNIKATNVLPIGDMNFEDAAMIEPLAVAMHGVLNIGVQVGD
                     TVAVMGSGTMGQLVIQGLKIAGAGTIIAVDISDNKLRESKELGADIIINAKDINPVEK
                     IKELTGGKGVDIALECAGSKITQEQCLLITKKKSKIGFLGIAYSDITLSEEAFENIFR
                     KELELKGFWNSYSAPFPGQEWTKGINLVNEGKIKLKEMVSHRFSLEDTYKAFEMIRDR
                     KEEFNKILILPQGVEK"
     gene            3589..4620
                     /locus_tag="PC471_002584"
     CDS             3589..4620
                     /locus_tag="PC471_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012986518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HDF2789702.1"
                     /translation="MKAVVKTKPGYDNVEVLEVEEPKATGDKVKIKVEYSGICGSDIH
                     SFKGEYANIKAPVTLGHEFSGIVVEVGEDVKNIKVGDRVTSETTFATCEKCIYCETKD
                     YNLCPTRKGIGTQVNGSFAEYVLSREESIHVLPENVSLLSAALTEPLACCVHAALEKT
                     TIESSDTVLIIGPGPIGLLLAQVVKSQGATVIMSGVTKDKERLEIAKNLGVDRTVNVM
                     EESLADVVNEMTDGNGVNKGFDCSGFMPAVNEALRLLRKKGTFVQVGIFAKKLNEMDQ
                     EAIIQRELQYIGSRSQKPSSWITALDLMANGKVNTEALVTRTVGLDEFKEGITALMNG
                     EEIKVAVKS"
     gene            4712..5524
                     /locus_tag="PC471_002585"
     CDS             4712..5524
                     /locus_tag="PC471_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HDF2789703.1"
                     /translation="MSTLRDHSNNIRKNIIKMVAEAKSGHPGGSLSIADMLTVLYFDK
                     MNVCAENPKMADRDRFVLSKGHAAPALYATLAEKGFFPEEELITLRKFGSKLQGHPDM
                     KKVAGIDMSTGSLGQGLSAANGMALAGKLDNKDYTVYTILGDGEIQEGQIWEAAMTAS
                     HYKLDNLIAFVDLNGLQIDGSNEEVMNVSPVDDKFKSFGWNVIVINGHSFEEIGNAVD
                     EAKKVTGKPTVIIAKTVKGKGVSFMENNAAWHGTAPNAEQAEQALKDLEGGL"
     gene            5527..6447
                     /locus_tag="PC471_002586"
     CDS             5527..6447
                     /locus_tag="PC471_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HDF2789704.1"
                     /translation="MGIATREAYGQVLKELAENKDIVVLDADLGKATKSISFKEVAPD
                     RFFDMGIAEGDMIGTAAGLATCGKIPFASTFAIFAAGRGYEQIRNSVAYPNLNVKIAA
                     THAGVTVGEDGGSHQAIEDISLMRGIPNMVVLNPADALEARQAILTSIDYNGPVYIRL
                     GRAATPDVNSENYKFEIGKGTVLREGSDITVIATGIMVAKALEAAEELAKEGINVEVV
                     NISTIKPLDEALIKETAKKTGKVVTAEEHSIIGGLGSAVCEALAETKDVVVRRIGVKD
                     VFGQSGTPADLLKHYGLTTEDIVKNIKELL"
     gene            6622..7068
                     /gene="rpiB"
                     /locus_tag="PC471_002587"
     CDS             6622..7068
                     /gene="rpiB"
                     /locus_tag="PC471_002587"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HDF2789705.1"
                     /translation="MKISIGCDHGGYELKEYIKENLINKGIEVEDVGTNSKESVDFPI
                     YAKKVSQNVQSKKSDLGILCCGTGIGMSIAANKFKGIRAAVVSDCFSAKATREHNNTN
                     VLCLGERVIGKGLAMRIVEEWLNSSYNGERHARRLQMIDDIEKENL"
     gene            7098..7763
                     /locus_tag="PC471_002588"
     CDS             7098..7763
                     /locus_tag="PC471_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HDF2789706.1"
                     /translation="MSIICASIMCADQMKLKEELEALEEAGVKLLHCDVMDGIFVKNL
                     AMGPELLKSISENTTIPLDIHLATETPDKYIDMMSYIKPKYISFHVESSTNVKADIQK
                     LRNYGIGPVLAISPQTSVDKIEEYISLVEGILVMTVNPGFAGQKFNLSVLDKLDKLTE
                     ILKDYDNPPFIEVDGNINKDTIKLMNGKKVDIYVVGTSALFNDKPPISYKDKIEELKE
                     SIK"
     gene            7873..9375
                     /locus_tag="PC471_002589"
     CDS             7873..9375
                     /locus_tag="PC471_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HDF2789707.1"
                     /translation="MGDLYKLQNGTDIRGIAYENDSKEVNLTVEEVKKIAKAFHIWLK
                     EKTKKDKVTVAIGTDSRITGSQFRSTVIETLTNDDCNVIDCEIATTPAMFMTTIMDGY
                     NSDGAIMITASHLPYYYNGLKFFTESGGLEKTDIKEMLDIAVKNDSVDYEEVNKKGEV
                     IVKNLIEDYSNLLIDKIRKGVNSSKNYEKPFSGLKILVDAGNGAGGFFAEKVLHILGA
                     DTTGSQFLNPDGMFPNHIPNPENKEAMESICKAVLDNKSDLGIIFDTDVDRAAIVGKN
                     GKPINKNALIAVISSIVLEEHPKTAIVTDSITSEGLAKFINELQGRHHRFKRGYKNVI
                     NEAIRLNSEGEECHLAIETSGHAALKENYFLDDGAYLIAKILIKVAKLSLEGKTIEEL
                     IENLEEAQEEKEVRIGINKEDFRPYAEDILKDLDSHVKSIDGWSVAPNNFEGIRVNCD
                     ESNGDGWFLLRISLHEPLLALNIESNKIGGAEQIYSKLKLFLEKYDLKGL"
     gene            complement(9687..11279)
                     /locus_tag="PC471_002590"
     CDS             complement(9687..11279)
                     /locus_tag="PC471_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HDF2789708.1"
                     /translation="MVTKLKNLIINDSKAKFILLLLLFLLVSILFEAVYDLNNNFSYN
                     INYYYKEFALPYFYFFGTPVHLSKVELEFVCIGIILSSFLLTILCFTLYKKNKQTKVK
                     FLENVISLFSKVKIELTLILTLILLTLRFHAFTSNKMFYNTNLIIYNFIIISIVYIIY
                     ENIKYNNYNLKDMSITYVFFSNLLNMYNKKTINKKLAIVFLISIVVQAIIQYLVLIFF
                     MYIYYAEAIAIVILFTLVNTVFHFYIYNFLSVKFNYINNISKNIKNIENGNLKYKLEV
                     IGDDEISTLAKSINNITGGLETAVDANIKNERMKAELITNVSHDLKTPLTSILSYVDM
                     LKNNDFDRETINDYINILDKKSQRLKLLIDDIFEASKLSSGDVEFNITKTDIRELLIQ
                     SIVEFEDKIQNSSLDFIIETPEYAVFTMIDGKKTWRVFENLICNILKYSMPNTRVYID
                     MFIKEENIILTFKNISNDKLNLKPEELIERFRRGDISRKTDGSGLGLSIAQNIIELEN
                     AHMEIIIDADLFKVMLTFRRVN"
     gene            complement(11248..11964)
                     /locus_tag="PC471_002591"
     CDS             complement(11248..11964)
                     /locus_tag="PC471_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2789709.1"
                     /translation="MNQNILVVDDDREIVDAIEFYLRPDNFNVLKAYDGLEALDILIE
                     NDIKLIIMDVMMPNLDGLKTTLKIREKRNIPIILLSAKSEDMDKIIGLNMGADDYITK
                     PFNPLELTARVKSQLRRYINLGDFSNTNNSNDTNYILKSGGLELNTDTKIVSLDGEEI
                     KMTPLEYKILTLLMSRKGKIFSSHEIYENVWNEDAYSCERTIAVHIRRIREKIEINPK
                     EPKYLKVVWGIGYKIEKLNY"
     gene            complement(12504..13304)
                     /locus_tag="PC471_002592"
     CDS             complement(12504..13304)
                     /locus_tag="PC471_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HDF2789710.1"
                     /translation="MTFKNKKFTINKYKILSIILVLCLILTAFSSYVNYSHKKFKDYV
                     VFYVQHQDDEVLWAGSAIVNAIKERGKNHVFVVLVSTGCGISVFDKYKKYENLTNIQK
                     SEYRNREFLASLNSLGVIRENIILLPETNTNGNTDFNYMEKIALTFEEKFKNVTHIAH
                     TYKLDDHLQHLKNGSVIQNLYNAGKIKDAKYFVKPKFADKITFNNKIVYKAHSTEDYE
                     KVKNACQQYKIVDELEYREGIGYKSDHKSFDKLLNNKDVPAILHTPNL"
     gene            complement(13618..14349)
                     /locus_tag="PC471_002593"
     CDS             complement(13618..14349)
                     /locus_tag="PC471_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2789711.1"
                     /translation="MNIFDILRDYLLVQVDKYNLNMDMISIVSKSLSSKEAIGNTKRK
                     DFPIIVGKEIMLEADFKGAKGQAFTSTPSTFEGSLKDILSLDLHDNPHDRSLFIASLN
                     AVMKYLGKTDRTIHCKNNEPEICAKKFPEFIKMEFGNPKVAIIGYQPAIIDNIKDFFE
                     TRVLDLNPEFVDTIQYNVKIEDGIRDYEDVISWADLVICTGSTLCNNSIINFLSLNKP
                     VYYYGTTIAGASNILGLKRLCFCSK"
     gene            complement(14336..15031)
                     /locus_tag="PC471_002594"
     CDS             complement(14336..15031)
                     /locus_tag="PC471_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDF2789712.1"
                     /translation="MLKIKNLSLDLPNFKLQENSLNINNHEYFVLLGASGSGKTLFLE
                     TLAGRYPSAKGNVSYKENLISNLPPENRNIGFVYQNFELFPNMNVEDNIRFPLKLRKI
                     PIDEQILKANHLSKLMQIEDIKNRYPKNLSGGEKQRVAFARALIAEPDILLLDEPMSS
                     LDYITKKHISKILKDVYIKYKPTVIHVTHDISEAMFFAHKIGIMNNGKINHIFDLNEE
                     IKNKGESFFYEYI"
     gene            complement(15033..15824)
                     /locus_tag="PC471_002595"
     CDS             complement(15033..15824)
                     /locus_tag="PC471_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2789713.1"
                     /translation="MKNKFFTTLLTGFFAIVFLFIVSPLLMLIIKGISYVPICLKSVE
                     VQYAIILSIKTSLISTIVCLLLAIPVAYFLHITKLPFKKLIIQIINLPMSLPHLVSGI
                     ALLLLFGRMGIGDSIYKIFKLDFIFTKQGIVLAQVFVNLPLTIKILYTSLNESNEKMI
                     FVARTLGCNSWEAFRFIILPNLKTGIISATVMTWSRALGEFGAVAMIAGSTRMKTEII
                     PTSIYLNMSTGDIDIAIGIAVILIFISLTCLMLFEIFFNREVDKN"
     gene            complement(15834..16646)
                     /gene="modA"
                     /locus_tag="PC471_002596"
     CDS             complement(15834..16646)
                     /gene="modA"
                     /locus_tag="PC471_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HDF2789714.1"
                     /translation="MFKKLSVLALAMIISLSITACNSKENKANDESNTKVTTADDSKE
                     KETLFVYSGAGLKKPMDEIAKKFEKENNVKVEYSYAGSAQLIAQIETSHKGDVFIVGS
                     EPIYSKAFEKELVGEHKTVAHHTPAIVVPKGNPANIQKLEDLNKDGVKLILGDKESNA
                     IGKTTQKILEKNNLKSINDNVVSTAATVNEMIVQLTSSKADATIATKDSVFGNEDVEV
                     IEIAQDKNIDQLISAGIVNYSDKKDLANKFINYIESDEAKQIFKKYGFEPVK"
BASE COUNT         6402 a         2068 c         2703 g         5636 t
ORIGIN      
        1 aaccatttgt aaaaaacaaa aaaatcattt ctctaacaaa aaaaaccaca aaaaatataa
       61 aaaagtatgg ctagttaagt gattaacaag taaaaattaa aataaaaatc aaaaaaaatt
      121 tatcaatgaa agtcagctaa aatcctggaa gagagcatta tttttgggaa aacattttct
      181 tttgtttcac aaaaaaagcc actttttggc acacaacatg ctttatataa agtcaagaaa
      241 aactaaggta taagacaaag gcaattagga ggtgtaaata tggagtttat aaattttgta
      301 agtgataaag ttatagacct taaaatggat tgtaaaacga aagaagattt ctttaaagaa
      361 atacataata aagtttttga tttaggcttt gtaaaagaag aatttggaga aaaaatatta
      421 gcaagagaaa atgtatttcc aacaggttta aatttaggtg attatggagt agcaatacca
      481 catacagatg cggaatatat taaagagcaa tttatatcag tctgtacttt taatgaacca
      541 gtagtattta gttctatgga agaccaagat gaaaaagtac ctgtaaattt ggctttcgta
      601 ttaggactta atcaaccaca cagtcaatta tctgtattaa cagaattaat gggaattatg
      661 caaaataaag agttagttga taagttgatg agttctactg ataaagaaga agttttaaag
      721 acactaaagt ctttataaaa ataaaaaagt actaaaaata aaaaatataa aaataaaaat
      781 tattaggagg aaataaaaat gaaaaaaata ttagtagcat gtggagcagg aatagcaaca
      841 tcaacagtag tttgtgacaa ggtagagaga ttagttaagg aaaataatat acaagcagaa
      901 gttatacaat gcaaaatagc tgaatgtgca acaaagcaag aaggagcaga tttaatagta
      961 tcaacaacta tactaccaac aacttataat ataccagcaa taaaagcaac agcatatata
     1021 actggagtaa atacagcagc attagataaa aaaatattag atgctttaaa ataatcctat
     1081 catagataca ttgaaataat attttataaa acagaaaaag gaggatttat aaatggataa
     1141 attattagca gtagtacaat acattttaaa tatgggaccg acagtaatac taccaataac
     1201 aattgtagta ctaggaatga tatttagaat taagtttaca aaagctttaa agtcaggtct
     1261 tacaattggt ataggatttg tgggtataaa cttagttgta aacctattaa catcaacact
     1321 agggccagcg gctcaaggaa tggttgaaag attcggactt aatttaacag taatagatgt
     1381 tggttggcca acaacagcat cagcaacatg ggcttcacca gttgcaccaa tattaatacc
     1441 agtttgttta ttagtaaact tactattatt atacttcaat aaaacaaaga tattaaacgt
     1501 tgatatatgg aattattggc actttatagt agcaggtgca gttggatata tagtaacagg
     1561 aagttatgtt tgggctatat tctgtgcaat actaatggag ttattagtac tgtttattgc
     1621 agaaaagaca gcaaaaaatg ttcaggagtt ttatgattta gaaggtatag cattaccaac
     1681 tggttcaaca gcttcatttg ctccacttgg atatttagta ggtaaatttg ttgaaaaaat
     1741 accaggaata aataaaatac aagcagaccc agactctata caaaaaagat ttggtatatt
     1801 tggagaacca atgatgatgg ggattatact aggtataata cttggagcac ttgcgggata
     1861 tgatgtagca ggaatattca atgtaggtat atcaatgggt gcagtaatgt tacttatgcc
     1921 tagaatggtt agaatattaa tggaaggttt aattccaatt tcagaatcaa taagagattt
     1981 cttacaagca aaatatggag acagagaact ttatataggt ctggatgcag cagtagcaac
     2041 tggacatcca gcagttatat caacagctct tatacttgtt cctataactt tgttcttagc
     2101 agttatacta ccaggaaaca aagttttacc atttggagac ttagcgacaa taccatttta
     2161 tatggcattt atagtgtgtt ttagaaaagg taatatagtt caatcagtta taacaggaac
     2221 aatagtaata gctatatcac tttatatggc tacaaacttt gcagatgttc atacacaatt
     2281 attacaacaa gcaaatatga tgccagaagg aacaacaatg gttacaagta tagatacagg
     2341 tggaaacttg ctaaaatggg gaatattgaa attatctcaa ttagtttctt catttggaat
     2401 attctagtat gtagttaata gagattactc ttttttagta aagtatgcga aaatttaaca
     2461 tttagcagct actaaaaaag agtaatattt tttataaatt tttaaaaata caaaatacaa
     2521 gaggaggaga tttatcgtga aagcagcagt attacatggc acaaatgata tgagatttga
     2581 agatatagaa ataaaaccat gtgaaagtga tgaagtaaaa ataaaagtta tggcagcagg
     2641 aatatgtggg tcagatcctc caagagtatt aaaacattgg aaatatccag ttccagctat
     2701 accaggacat gagttttctg gagtaattgc tgaagttggt aaggatgtaa aaaatgtaaa
     2761 agttggagat agagtagttg caattccttt tataccttgt aatgaatgtg aatattgtaa
     2821 aagaggatta ttttcattgt gtgatgacca tggaatgtta ggagctaaaa gttttggagc
     2881 ttttgcagag tatgtaaata taaaagctac aaatgtactt ccaataggag atatgaactt
     2941 tgaagatgct gcaatgatag agccactagc agttgctatg catggagtac ttaatatagg
     3001 tgttcaagtt ggagacactg tagctgtaat gggtagtgga acaatggggc agttggtaat
     3061 acaaggactt aaaatagcag gggcaggaac aattatagca gttgatatat ctgataataa
     3121 attaagagaa tcaaaggaat taggggctga tataataatt aatgctaaag atattaaccc
     3181 agtagaaaaa ataaaagaac ttactggtgg aaaaggtgtt gacatagctc ttgaatgtgc
     3241 aggttcaaaa ataactcaag aacaatgttt acttataact aaaaagaaaa gtaagatagg
     3301 atttttaggg atagcatatt cagatataac tttatcagaa gaagcatttg aaaatatatt
     3361 tagaaaagaa ctagaattaa aaggtttttg gaactcttat tctgcaccat ttccaggaca
     3421 agaatggact aagggaatca atctagttaa tgaaggaaag ataaaattaa aagaaatggt
     3481 atctcataga ttctcacttg aagatactta caaagcattt gaaatgataa gagatagaaa
     3541 agaagaattt aacaaaatat taattttacc acaaggggtt gaaaaataat gaaagcagta
     3601 gttaaaacta aaccaggcta tgataatgta gaagttttag aagtagaaga accaaaagct
     3661 acaggagaca aggttaaaat aaaagtagaa tatagtggta tatgtggttc agatatacat
     3721 tcatttaaag gtgaatatgc aaatataaaa gccccagtaa cattaggtca tgaattttct
     3781 ggaattgttg tagaagtagg agaagatgtt aaaaatataa aagtaggaga tagagttaca
     3841 tcagagacta cttttgcaac ttgtgagaaa tgtatatatt gtgaaactaa agattataac
     3901 ttatgcccaa ctagaaaagg tattggtact caagtaaatg gaagttttgc tgagtatgta
     3961 ttatcaagag aagaaagcat acatgtactt cctgaaaatg tatctttatt atcagcagcc
     4021 cttacagagc cactagcttg ttgtgttcat gcagctcttg aaaagactac tatagaaagt
     4081 tctgatacag ttttaataat aggaccagga ccaattggtt tattattagc tcaagttgtt
     4141 aaatctcaag gtgctacagt tataatgtct ggtgttacaa aagataagga aagattagaa
     4201 atagctaaaa atcttggagt agatagaact gtaaatgtaa tggaagaaag tttggcagat
     4261 gtagtgaatg aaatgactga tggaaatgga gtaaacaaag gatttgattg ttctgggttt
     4321 atgccagcag ttaacgaagc cttaagactg cttagaaaaa aaggtacttt cgtacaagtt
     4381 ggaatctttg ctaaaaaatt aaatgaaatg gaccaagaag caataatcca aagagagtta
     4441 caatatatag gaagcaggtc tcaaaagcca agttcttgga ttacagcatt agatttaatg
     4501 gcaaatggga aagtaaatac agaagcttta gttactagaa ctgttggttt agacgaattt
     4561 aaagaaggta ttacagcttt aatgaacggg gaagaaataa aagtagcagt aaaatcttaa
     4621 aaaaacattt gtattatttt aaaaaaaatt gtaaaatcta ctgtaggata aaaaaatgta
     4681 aggtcttaaa aaataacagg aggaagaaag tatgagcact cttagagatc attctaataa
     4741 tattagaaaa aacataataa aaatggttgc agaagctaaa agcggacatc caggtggttc
     4801 attatcaata gctgatatgt tgacagtatt atattttgat aagatgaacg tatgtgcaga
     4861 aaatccaaaa atggctgata gagatagatt tgttctatca aaaggtcatg cagcaccagc
     4921 tttatatgct acattagcag aaaaaggatt tttcccagaa gaagaattaa taacactaag
     4981 aaaatttggt tctaagttac aaggtcaccc agacatgaaa aaagtagcag gaatcgatat
     5041 gtctactggt tctcttggtc aagggctatc tgcagcaaat ggtatggcac ttgcaggaaa
     5101 attagataac aaagactata cagtatatac tatattaggt gatggagaaa tccaagaagg
     5161 tcaaatatgg gaagctgcaa tgacagcatc acattacaaa ttagataatt taattgcatt
     5221 tgttgactta aatggattac aaatagatgg ttctaatgaa gaagtaatga atgtaagtcc
     5281 agtagatgac aaattcaaat cttttggatg gaatgtaata gtaataaatg gacattcttt
     5341 tgaagaaata ggaaatgcag ttgatgaagc taaaaaagtt acaggaaaac caactgtaat
     5401 aatagctaaa acagtaaaag gtaaaggtgt ttcatttatg gaaaataatg ctgcatggca
     5461 tggaacagct ccaaatgcag aacaagcaga gcaagcattg aaagatttag aaggaggtct
     5521 ataggaatgg gaatagcaac tagagaagct tacggacaag tgttaaaaga acttgcagaa
     5581 aataaagata tagtagtatt agatgcagat ttaggaaaag ctacaaaaag tatatcattt
     5641 aaagaagttg ccccagatag attttttgac atggggatag cagaaggtga catgattggt
     5701 acagcagcag gtcttgctac ttgtggaaaa ataccttttg cgagtacttt tgcaatattt
     5761 gcagcaggaa gaggctatga gcaaataaga aactctgtag catatccaaa tttaaatgta
     5821 aaaatagcag ctactcatgc aggtgtaaca gtaggtgaag atggtggtag ccatcaagct
     5881 atagaggata tatctctaat gagaggtata ccaaatatgg ttgtattaaa tccagcagat
     5941 gctttagaag caagacaagc tattcttaca tctattgatt ataatggacc tgtttatata
     6001 agattaggta gagctgcaac tcctgatgta aacagtgaaa attataaatt tgaaatagga
     6061 aaaggaactg ttttaagaga aggttctgac ataacagtaa tagctacagg aataatggta
     6121 gcaaaagcct tagaagcagc agaagaactt gctaaagaag gtataaatgt agaagttgtt
     6181 aatatatcta caataaaacc attagatgaa gcattaataa aagaaactgc taagaaaact
     6241 ggaaaagtag ttacagcaga ggagcacagt ataataggtg gtttaggttc agctgtatgt
     6301 gaagctttag cagaaactaa agatgtagta gttagaagaa ttggtgttaa agatgtattt
     6361 ggtcaatcag gtacaccagc tgatttatta aaacactatg gtttaactac agaagatata
     6421 gtaaaaaata taaaagagtt gttatagata taaatatatc taaataacaa attgatttga
     6481 cataagtcaa ttaaagctgt cttaaaaata gaaagaaatt tctacaatgg gacagcttta
     6541 tttttacaat actttatttt tatgataatt ataaaatagt ctgaaagtaa tcaaaaaaat
     6601 tataaaaaga ggtgctattt aatgaaaata tctattggat gtgaccatgg tggttatgaa
     6661 ttaaaggaat atataaagga aaatttaata aataaaggta tagaagttga agatgtgggt
     6721 acaaattcaa aagaaagtgt agattttcca atatatgcaa aaaaggtaag tcaaaatgtt
     6781 caaagtaaaa aatctgattt aggcatactt tgttgtggaa caggaatagg tatgtctatt
     6841 gcagcaaata agtttaaagg aatcagagca gctgttgttt ctgactgttt ttcagctaag
     6901 gcaactagag aacacaataa tacaaatgta ctttgtttag gagaaagagt tattgggaaa
     6961 ggattagcaa tgagaattgt tgaagaatgg ttaaattcaa gttataatgg agaaagacat
     7021 gcaagaagat tacaaatgat tgatgatata gaaaaagaaa atttataaat atataattta
     7081 taacaagggg gagcgttatg tctattattt gtgcttcaat aatgtgtgca gaccaaatga
     7141 agttaaaaga ggaattagaa gctcttgaag aagcaggtgt aaaattactt cattgtgatg
     7201 ttatggatgg aatatttgtt aaaaacttgg ctatgggacc agagttatta aaatcaataa
     7261 gtgaaaatac aacaatacct ttagacatac acttagccac agaaactcca gataaatata
     7321 ttgatatgat gtcttatata aagccaaaat atatttcttt tcatgtagaa tcaagtacaa
     7381 atgtaaaagc tgatatacaa aagttaagaa attatggtat agggccagtg ttagcaataa
     7441 gccctcaaac aagtgtggat aaaatagaag aatatataag tttagttgag ggtatcttag
     7501 taatgacagt aaatccaggg tttgcaggtc aaaagtttaa tctttctgta ttggataagt
     7561 tggataaact tacagagata ctaaaagatt atgataatcc tccatttata gaggttgatg
     7621 gaaatataaa taaggatact attaaactta tgaatggtaa aaaagtagat atatatgttg
     7681 ttggtacttc agctttattt aatgataagc ctccaatttc atataaagat aaaatagaag
     7741 aactaaaaga aagtataaag taaccatgta ataaaattat aatatgtttc tgtttaatag
     7801 taaaaattta ggtggaaaca agaaatttta taatatttaa taaaaatttt ggttgagtaa
     7861 ggagaatttt ttatgggaga cctatataaa ctacaaaatg gtactgatat tagaggaata
     7921 gcgtatgaaa atgattctaa agaagttaac ctaactgtag aagaagtaaa aaaaatagct
     7981 aaagcgtttc atatttggtt aaaagaaaag actaagaaag ataaagtaac tgttgccata
     8041 ggaactgatt ctaggataac aggaagccaa tttagaagta ctgttattga aactttaact
     8101 aatgacgatt gtaatgtaat tgattgtgag attgccacaa ctccagctat gtttatgaca
     8161 actattatgg atggatacaa ttctgatggg gcgattatga ttacagcaag tcacttacct
     8221 tattactata atggtttaaa gtttttcaca gaaagtggag gtttagaaaa aactgatata
     8281 aaagagatgt tggatatagc tgttaaaaat gatagtgtag actatgagga agtaaataaa
     8341 aaaggtgaag ttatagtaaa aaatttaata gaagattatt ctaatttatt gatagataaa
     8401 attagaaaag gtgtaaattc aagtaaaaat tatgaaaagc ctttttctgg gcttaagata
     8461 ttggttgatg caggaaatgg ggctggtgga ttctttgctg aaaaagtttt acacatactg
     8521 ggagcagata cgacaggtag tcagtttttg aacccagatg gtatgttccc aaatcatata
     8581 cctaatcccg aaaacaaaga agcaatggaa agcatatgta aagctgtgtt agataataag
     8641 agcgacctag gaataatctt tgatacagat gtagacagag ctgctattgt aggtaaaaat
     8701 ggcaaaccaa taaataaaaa tgctcttata gctgttatat catcaatagt attagaagaa
     8761 catccaaaaa cagctatagt aacagattcg attacttctg aaggacttgc taaatttata
     8821 aatgaattac aaggaagaca tcatagattt aaaagaggat ataaaaatgt aataaatgaa
     8881 gctataagat tgaatagtga aggtgaagag tgccatttag ctatagaaac gtctggacat
     8941 gctgctttaa aagaaaatta tttcttagat gatggggcat atttaatagc taaaatttta
     9001 ataaaagtag caaagctatc cttggaagga aagactatag aagaattgat agaaaacctt
     9061 gaagaagctc aagaagagaa agaagttaga ataggcatta ataaagaaga ttttagacca
     9121 tatgcagagg atatattaaa agatttagat tctcatgtca agagtattga tggatggtct
     9181 gtagcaccaa ataattttga aggcataaga gttaattgtg atgaatcaaa tggagatgga
     9241 tggtttttac ttagaatatc actacatgaa ccattattag ctttaaatat tgaatcaaat
     9301 aaaattggcg gagcagaaca aatttattct aagttaaaac ttttcttaga aaaatatgat
     9361 ttaaaaggtc tttagggtaa atagaaagaa tatatttata ttgaagaata aatgtaaatt
     9421 tgtacctaag attacaaata ttacaaaatt gtaatcttag gtaacagaga tgtaactgat
     9481 tatggaaaca ctatgtgata ttatagatac agagagatga aatatgtgat gaattataga
     9541 gaaatataat ttaaaatgta ttaatatcac accaaaaagg tataatcaaa aaatatgtaa
     9601 atataaaccc caacatttag aagaagacct ctaaggaggt ttttttcttg tttaaataac
     9661 ttaaaatata tttttatatc aatatatcaa ttaactcttc taaaagtaag cattacctta
     9721 aataaatcag catctataat tatttccatg tgtgcatttt ctaattcaat tatattttga
     9781 gcaattgata aaccaagacc agagccatct gtttttctag atatatcacc ccttctaaat
     9841 ctttctatta actcttctgg ctttagattt agtttatcat ttgaaatatt tttaaacgtt
     9901 aatattatat tttcttcctt tataaacata tctatgtaga ccctagtgtt aggcatagag
     9961 tattttagta tattgcaaat tagattctca aaaactctcc aagttttttt accatctatc
    10021 atggtaaaaa cagcatattc aggtgtttct attataaaat ctaaactgct attttgaatt
    10081 ttgtcttcaa actctacaat actttgaatt aaaagttctc ttatatcggt ttttgttata
    10141 ttaaattcta catctccact acttagctta gatgcttcaa atatgtcatc tattaataat
    10201 tttaatcttt gtgatttttt atctaaaata tttatatagt catttatagt ctctctgtca
    10261 aagtcattat tttttaacat atcaacatag cttaatatag atgtaagtgg agtttttaag
    10321 tcatgagata cgtttgtgat aagctctgcc ttcatccttt cattttttat attagcatct
    10381 acagcagttt caagaccacc tgttatatta tttatacttt tggctaaagt agatatttca
    10441 tcatctccta taacttctaa tttatacttt aaattgccat tttctatatt ttttatattt
    10501 tttgatatgt tatttatgta attaaactta acagataaaa agttgtatat ataaaaatga
    10561 aaaactgtgt ttacaagtgt aaataaaatt actatagcta ttgcctcagc ataatatata
    10621 tacataaaga atatcaaaac aagatattgg ataatagctt gtacaactat agatattaaa
    10681 aatactatag ctaatttttt atttatagtt tttttattat acatattaag caagtttgaa
    10741 aaaaatacat atgttataga catatctttt agattatagt tattatattt aatattttca
    10801 tatattatat agactataga tataattata aaattgtaga ttattaaatt agtgttgtaa
    10861 aacattttat tacttgtaaa tgcatgaaac cttaaggtta atagtatcaa agttaatata
    10921 agtgtaagtt ctatttttac cttagaaaaa aggcttatga cattctctaa gaatttgact
    10981 ttagtctgtt tatttttttt atacaatgta aaacatagta tagtcaataa aaaacttgat
    11041 aatataatac ctatgcagac aaactctaat tctacttttg acaaatgtac aggagtaccg
    11101 aaaaaataaa aataagggag agcaaactct ttataataat aattaatatt ataagaaaag
    11161 ttattattca aatcataaac tgcttcaaat aatatagaaa ctaatagaaa cagtaataat
    11221 aataaaataa attttgcttt agaatcatta ataattaagt ttttcaattt tgtaaccaat
    11281 gccccacacc acctttaaat attttggttc ttttggatta atttcaatct tttctctaat
    11341 tcttctaata tgaacagcaa tagttctctc acaactataa gcatcctcat tccagacatt
    11401 ttcataaatc tcatgagaag agaaaatttt accttttcgt gacataagaa gtgtaagtat
    11461 tttatattca agtggtgtca ttttaatttc ttcaccatct aaagaaacta tttttgtatc
    11521 tgtatttagc tctaggccac cactttttag aatataatta gtatcatttg agttatttgt
    11581 attactaaaa tcacctaaat ttatatacct tctaagttga gattttactc gagcagtaag
    11641 ttctagtgga ttaaatggtt tagttatata gtcatcagca cccatattaa gacctataat
    11701 tttgtccata tcttctgatt ttgcagataa gagaattatt ggtatattcc ttttttctct
    11761 tattttaagt gtagttttaa gcccatctaa attgggcatc attacatcca ttattattaa
    11821 cttaatatca ttttcgatta gtatgtccaa tgcttcaagt ccatcatatg cctttaaaac
    11881 attaaagtta tcaggtctca aatagaactc tatagcatca actatctctc tatcatcatc
    11941 tacaaccaat atattttgat tcaacttatt aacccccttg aaattttatt tttaaccaat
    12001 ttttatttta taccaaaaag aggtattaga aaacagtaag cccgttcatt atgaaataaa
    12061 aactagtact tttaatatat tgtcaatgca tatagtaaat ttaaaagtat agacgcccat
    12121 attaatctga aggtaaaagt ttatgaaaat atctagggta atctatcaca ctccttatgt
    12181 tattttggtt tattaatttt ttaggtttaa acatatatta acaaatataa ataacaaatg
    12241 agtttcataa ttattaagaa tttataacaa atatttattt taatgtagtt taatataaat
    12301 aggtagctta atgtagatat gtataaagca tttaataagg cttataggtt ttaatgatga
    12361 tatctttata tattttaaga gatgatgaaa ttagatatgg gtaatagttg tatgagaaaa
    12421 agtagtatag cacaaaaagt gctatatcac aaaatctcaa ataatttcct ttttataact
    12481 taaattaaaa gaataaatat ttcttataaa ttaggtgtat gaagtatagc aggaacatct
    12541 ttattattta ataacttatc aaatgattta tggtcagact tatagccaat accttctcta
    12601 tattctaatt catcaacaat tttgtattgt tgacaggcat ttttaacctt ttcatagtct
    12661 tcagtagaat gagctttata aactatctta ttattaaaag ttattttatc agcaaattta
    12721 ggttttacaa aatatttagc atcttttatt ttaccagcat tatataaatt ttggataaca
    12781 gaaccatttt ttaaatgttg taaatggtca tctaacttat aagtatgtgc tatatgagtt
    12841 acatttttaa atttttcttc aaatgtaaga gcaatttttt ccatgtaatt gaagtcagta
    12901 ttaccatttg tatttgtttc tggtaataat attatatttt cccttattac accaaggcta
    12961 ttaagagaag ctaaaaattc tctatttcta tattctgatt tttgtatatt tgttaggttt
    13021 tcatattttt tatatttatc aaagacggat ataccacatc cagtagatac taaaaccaca
    13081 aatacatgat tttttcctct ttcttttata gcattaacaa tagcactccc agcccacaac
    13141 acttcatcat cttgatgctg aacatagaag acaacataat ctttaaattt tttatgacta
    13201 taattcacat agctactaaa agcggttaaa atcaaacata aaactagaat aatagataga
    13261 attttatatt tatttatagt aaatttttta ttcttaaatg tcatttaatc atctcctgaa
    13321 aagttgtctt agttatatta tcaaacaaag tactaaaaat tatttcttaa atgctgaaaa
    13381 atatagagta gattaatgga ataaaatttt tttattagtt taaaataaag aaaacattta
    13441 tgtataagac taatcaaaat attaatgatt gataggaatt gagatttaaa gggattgtac
    13501 atttattcgg gattcatttt aatagaattt tactttagta cttagaataa aagtaataac
    13561 tttttaaatt taaagagtac acatatatat ttgtgtactc cttgatgata tgattgctta
    13621 tttactacaa aaacagagtc tttttaaacc taaaatattt gatgcaccag ctatcgtagt
    13681 tccataatag taaactggtt tgttaagtga tagaaaattt ataattgagt tattacataa
    13741 tgtacttcct gtgcaaatca ctaaatcagc ccaagatatt acatcttcat aatctcgtat
    13801 accatcttca atcttaacat tatattggat agtatccaca aattcaggat ttaaatctaa
    13861 tactcttgtt tcgaaaaagt ccttaatatt atcaattatg gctggttgat atcctataat
    13921 tgctactttt ggattaccaa attccatttt tataaactca gggaattttt ttgcacatat
    13981 ctctggctcg ttatttttac aatgaatggt tctatccgtc ttgcctaagt atttcataac
    14041 tgcatttaat gatgctataa aaagacttct atcatgagga ttgtcatgta aatctaatga
    14101 taatatgtct tttaaagaac cttcaaatgt agatggagta gaagtaaatg cttgtccttt
    14161 agctccttta aagtcagctt caagcattat ttctttacca actataattg ggaaatcttt
    14221 acgcttagta tttccaattg cttcttttga agatagagat ttggacacaa tacttatcat
    14281 atccatattt aaattatatt tatcaacttg tactaataga taatctctca aaatatcaaa
    14341 tatattcata gaaaaaagat tctcctttat tttttatttc ttcatttaaa tcaaaaatat
    14401 gatttatttt tccattgttc attattccta tcttatgagc aaaaaacatt gcttcagaaa
    14461 tatcatgagt aacatgaatc actgttggtt tgtattttat gtagacatcc ttcaaaattt
    14521 tgcttatatg tttttttgta atataatcca atgaactcat aggttcatct aaaagtaata
    14581 tatcaggttc agctattaag gcacgtgcaa aagcaactcg ttgcttttca cctccactta
    14641 aatttttagg atacctattt tttatatctt ctatctgcat taatttagat aagtggttag
    14701 ccttaagaat ctgttcatct ataggtattt ttctcaattt aagtggaaac cttatattat
    14761 cttcaacatt catatttgga aataattcaa aattttgata tacaaatcct atatttctat
    14821 tttctggagg taagtttgat attaaatttt ctttgtatga tacatttcct ttagcagatg
    14881 gatatcttcc tgctaatgtt tctagaaata gagttttacc tgacccacta gctcctaata
    14941 aaacaaagta ttcatgatta ttaatattta aactattttc ttgtaattta aaattaggta
    15001 aatcaagaga taaatttttt atttttaaca tattaattct tatcaacctc tctattaaaa
    15061 aatatctcaa aaagcatcaa gcatgtaagt gagataaata tcaaaattac agctattccg
    15121 attgcaatgt ctatatcacc agtagacata tttaaatata tagatgtagg aataatttca
    15181 gttttcatac gtgtagaacc tgctatcata gctactgcac caaattcacc aagtgctctt
    15241 gaccaagtca ttacagttgc agatatgatt ccagttttta gatttggaag aattataaat
    15301 ctaaatgctt cccaactatt acatccaagg gtacgtgcaa caaatatcat cttttcatta
    15361 ctttcattta gtgaagtgta aagtatttta atagtaagtg gaagatttac aaatacttgt
    15421 gctaaaacta taccttgctt tgtaaatata aaatcaagct taaaaatctt ataaatggaa
    15481 tctccaatcc ccattcttcc aaacaaaagt aaaagagcaa ttcctgatac taagtgtgga
    15541 agagacatag ggagatttat tatctgtata atcagttttt taaatggtaa tttagtaata
    15601 tgtaaaaaat atgctactgg aatagctaat aaaaggcata ctatagtaga tattaaagat
    15661 gttttaatac taagtattat tgcatactgt acttccactg attttaagca tataggaaca
    15721 taacttatcc ctttaatgat taacataaga agtggtgata caataaacaa aaagactata
    15781 gcaaaaaatc cagtaagtag agtagtaaaa aatttatttt tcaagtttaa tcactactta
    15841 acaggttcaa aaccatattt tttaaatatc tgtttagctt cgtctgactc tatataattt
    15901 ataaatttat tagctaaatc ttttttatca ctatagttta cgattcctgc tgatataagt
    15961 tggtctatat ttttatcttg ggcaatttct ataacttcta catcttcatt gccaaagacc
    16021 gaatcttttg tggctattgt agcatcagct ttacttgatg ttaactgtac aatcatttca
    16081 tttacagttg cagctgtaga gacaacatta tcatttatag attttaaatt atttttttca
    16141 agtatttttt gtgtagtctt acctatagca ttagactctt tgtctcccaa aataagttta
    16201 actccatctt tatttaagtc ttccaatttt tgaatattag ctggatttcc tttaggtaca
    16261 actattgcag gtgtatgatg tgcaaccgtt ttatgttcac ctacaagttc tttttcaaag
    16321 gcttttgaat aaattggttc agaaccaaca ataaatacgt cccctttgtg acttgtctca
    16381 atttgagcta ttagctgtgc agaacctgca tagctatatt caactttgac attattttct
    16441 ttctcaaatt tttttgctat ttcatccatt ggtttcttta aacctgcccc tgaatagaca
    16501 aaaagagtct ctttttcttt tgaatcatca gctgttgtca ctttagtatt agattcatca
    16561 ttagctttgt tttctttaga attacatgca gtaatactta atgaaataat catagctaaa
    16621 gctagtactg aaagtttttt aaacataaga tattccccct tagtaaatta atgtaaattc
    16681 ttttttagta tatcatacta agaaaacgat gtctaagttt ttgttatgtt ttataatgtt
    16741 ttgttatgtt ttgttatgta tagttaagaa aaaatctatt tataatgtta tttctatagg
    16801 tagttattt
//
LOCUS       DAMJYR010000084        16580 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.085, whole genome shotgun sequence.
ACCESSION   DAMJYR010000084 DAMJYR010000000
VERSION     DAMJYR010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 16580)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16580)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16580
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.085"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(228..1757)
                     /locus_tag="PC471_002597"
     CDS             complement(228..1757)
                     /locus_tag="PC471_002597"
                     /inference="COORDINATES: protein motif:HMM:NF012862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HDF2789715.1"
                     /translation="MKSLKALIVGVSKYSIVKNKDLLFCKNDIIEINNVLHKRLGITS
                     NNIRTLGMEYEVRKDLFFKTFQEISDSIGDDDTFIFYFSGHGCICNNGVHYLVLSDEL
                     VSIQFVIDFIEGLNCKNKIVFIDCCFAGKFNVSDKQKINIKDIFSLFDRKGCAVLVSS
                     SEYQESFSLPDSSISRFTSLLSEALRNEYIVRKGELSLRAISKYINLRMSILNKNYIN
                     CKQNPIFRSNILGDIYFNVKDYKSYIKKKVFQEHDDYIIYNVKSLHISIIKRYSVEVI
                     LKNKFSYCDISEISKEVFDIVKSVEVYNSEIGEDRLKGKDANIIWVYFGKDEIDMING
                     IYMCKTTWIDKYQEKDIWYSVNGKNKFFINGVHTETYDNYESLKSYISQNTITKEKAV
                     YEVKEILTNMINISEEIISFYNEYENKKICEDNIFQIIDSRKKKMDECYNKVINLDFP
                     PIEIKDWFDKCINLVSTIQNFGVYWDHSRTEENRKQCMEMNIEDYYKDLREVIRLSEE
                     I"
     gene            complement(2456..3886)
                     /gene="dcm"
                     /locus_tag="PC471_002598"
     CDS             complement(2456..3886)
                     /gene="dcm"
                     /locus_tag="PC471_002598"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: protein motif:HMM:NF024835.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007157505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HDF2789716.1"
                     /translation="MKKITIDKNRVRKIMKEKNIRSQNQLALNMGITKNQLSAILSEK
                     FTPIKSNVEKLCEVLDIGFDEIMCLEDVNEKQLKFTNICSSNKDYIEHRDCTYKELSE
                     YELNPRERNKNNKHIDISNVLAKRAYTCVELFAGAGGLALGLEKAGFKEVGLVEWDKY
                     ACDTLKLNRPDWNVIQGDIVKIAENGIKNYIDNNIEIDLLSGGYPCQAFSYAGKKLGL
                     EDVRGTLFYSYAKILEELKPKVFLAENVRGLVSHDGGKTLKTMIDVFSEIGYNVKYEI
                     LKAVNYGVAQKRERVIIIGTRKDLSHVDFKFPKSFEYVATLRDALKNVPKSEGARYPE
                     RKKKVLDMVPPGGCWIDLPDEVARDYMGKSYYSGGGKRGMARRISWDEPCLTLTCSPA
                     QKQTERCHPDETRPFTTREYARIQSFPDEWEFTGSVSQVYKQIGNAVPVKLGKAIGLS
                     IVDYLNKIEKIYVTEEVAITEEEYVI"
     gene            4099..4815
                     /locus_tag="PC471_002599"
     CDS             4099..4815
                     /locus_tag="PC471_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002601887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HDF2789717.1"
                     /translation="MTELEKQEILDKAKDFFTNILVANHKKNTIKLNLKAFNINPFLV
                     KYLASFLTGSNKPEDIAKALIYPRVLGTSINTSFGTNLQKFCITTLDGFASTTSGIDI
                     EFIDCIDGRRKFCQIKAGPNTINKDDVKTIIDHFSSVKRLARTNGLSLSYNDLIVGVF
                     YGTENELNAHYKRINEEYPVVVGQDFWYRLTGDENFYFDLIDTIAQSALEEDCTVLLK
                     KTISNLADEISKDKSLFNQK"
     gene            complement(5060..5338)
                     /locus_tag="PC471_002600"
     CDS             complement(5060..5338)
                     /locus_tag="PC471_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2789718.1"
                     /translation="MYDEIIEIYFDYWINEHMYRKVLEKDEYYNDRCKEIKKVNKLLE
                     KNLSEEKKMDVLIELNDLFNSVIEIERELGYKLGFKAGARFMIELLNS"
     gene            complement(5586..6920)
                     /locus_tag="PC471_002601"
     CDS             complement(5586..6920)
                     /locus_tag="PC471_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDF2789719.1"
                     /translation="MDNMSNLVKNTEAEQSILGSILLDSRKIEEIEILISEDMFYHQE
                     HKHIFNSVKDIHKRDEPIDIITLTEDLKKKGLIDYVGGISYLTKLSTIVPTIGNVSHY
                     IEIVIEKYKNRNIIDKFNKFKLGEIDDNTLIYQLEKYVNEVNQKLVKKDSSITAISER
                     AFEYINDEIEEGIDIGIKFLDETIGGLYSGELITIAAKSGRGKTALALQIMRNVVFQG
                     KKVLFISGEMSDIQILFRNIASLTGISVVKMKNRNLNEEEIIKYINALSTLNQENNLY
                     INDSVNTISGIKREIKSIKPDVVIIDYLQILDYEGKEVGEAKFADLSRQIKKITLDFN
                     IPIIQLAQLNDELKDERPKGDRVMRSSKQIYMDSNSVIYIHRPTEKEALDYCKTMGIE
                     DIKELRRREKGFGANLSEIIVAKNRDGESGFRPYWYIGSLFSFKECKKVKEV"
     gene            complement(6933..7676)
                     /locus_tag="PC471_002602"
     CDS             complement(6933..7676)
                     /locus_tag="PC471_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2789720.1"
                     /translation="MSNLGWIKLHRKIKESPLYKDCNSKQRDILINLLLSVNHKPNKW
                     VYGGKEYEVKSGQLITSLESIANMCSKDVSIQNVRTALKKFERYGFLTNKSTNKNRLI
                     TIENWELYQSNEENQQTNQHAINKQLTTNKNVNNEKSKIYSEIIEKFNITCTELSKVK
                     KLTEARKKVINARIKEYDKETVLEIIHIVSKTSFLNGSNEKGWKANFDWIMSPNNFIK
                     ILEGNYQDKSINVKVDAEAYSPDDSQLKW"
     gene            complement(7697..8011)
                     /locus_tag="PC471_002603"
     CDS             complement(7697..8011)
                     /locus_tag="PC471_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2789721.1"
                     /translation="MADTNNLEVLKTLQATLNILVKQEQEAQEKEKGYKEIQTYRRDK
                     AQLITCKEASKIYPIGESKFRQLCHSKNKGFPCIWIDNRVYIIKNKLDNWFIENANGI
                     KL"
     gene            complement(8015..8209)
                     /locus_tag="PC471_002604"
     CDS             complement(8015..8209)
                     /locus_tag="PC471_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003450825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2789722.1"
                     /translation="MKLKLKRIEKGIKQYELAEKVGISRYYLSSLERGKSKNPSNMLM
                     LKLAQELDTSVEELFFDKEE"
     gene            8493..9209
                     /locus_tag="PC471_002605"
     CDS             8493..9209
                     /locus_tag="PC471_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF2789723.1"
                     /translation="MIKKISTALSIPINRLIIDESDLDTSTNNYTNLKELDLVVKTFD
                     NYSAKLIGNNISKFRNINNISLKELSEKINCSENEIINYENGTKMPRIEILKKIALAT
                     YTKIEDLFGFPLEEIKIDNQTILYRNPQTGYIIDNFLIDFINKDINIDNTPVSYYIDK
                     SEFDLVIHILKNYGYDVKTDYKTKFEIADNADTIKPISLSFSDFKDFSKNLNWVINGL
                     VENFIQENSNGLIEDEDEEY"
     gene            9306..10058
                     /locus_tag="PC471_002606"
     CDS             9306..10058
                     /locus_tag="PC471_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HDF2789724.1"
                     /translation="MNKSYIPKYTHKFPTNLPHGDKKYTLTYIRRMISEFVYDMGNLE
                     NNPFTFPEVQTLLDGITVGGHKLSDQNQILNIKSSWDYVIKLSNKENLSLNKDTICSI
                     HKIVAKDEALIVGDFRNGNIGIAGTTQYECIEATLLNDLFVSDINIINKITNPLEKAI
                     IINLWLSYCQFFYDGNKRTARLTSNLILISNDIGVLSIPAKHKQEYNTLMLNFYETLE
                     ADEVIKFLLEKCITFFDEYNYKSYKELFKNGN"
     gene            10048..11214
                     /locus_tag="PC471_002607"
     CDS             10048..11214
                     /locus_tag="PC471_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDF2789725.1"
                     /translation="MATKRANGEGSIGKYKDGWRSRIMIGYNEDGKPIRKEFYGKTQK
                     EVKDKLDAYKKQYYLSSDISDDKITLEHWFYNWLFEYRIKDLKPKSFERYESIYRNYI
                     KDTDLGNVKLKDLRVSHIQKYYNRLLDSNKSIPTIRQINTKLKTCLSEAEKQGLIQRN
                     YCTMVNIPVEKKENKLEILTLEQQKSFIKAIDGHKLEVLFLLALGTGLRLGELLGLKW
                     FDIDFKKSNLTVKRTLQRTYFIDKTGNRELKVLEQEPKTSNSYRTVPIPKDVLNKLKE
                     HKVNQNSDILKAGELYRNDNYVFCNELGAPIDDKRPLRNLKSILNSLDIEPIKFHGLR
                     KTYATRLFENDVPPKTVQVLMGHYDISITLNIYTQVMEDKKIEAVEKLDKIFSL"
     gene            complement(11331..11561)
                     /locus_tag="PC471_002608"
     CDS             complement(11331..11561)
                     /locus_tag="PC471_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2789726.1"
                     /translation="MEANKTLLQSLYKKIILEFSKQTGKSLEESMDYLYTSETYKLIS
                     EGISDMHCKGHIYLAQELMLENGLMYHKGYPR"
     gene            complement(11810..16381)
                     /locus_tag="PC471_002609"
     CDS             complement(11810..16381)
                     /locus_tag="PC471_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007062489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIR2 family protein"
                     /protein_id="HDF2789727.1"
                     /translation="MTFEEIMEFNKPYNQKNFDELVNAIKDGYVVPYIGAGMSMLFKD
                     IYPSWSTFLNNTSEEYLEDEDKILFEKIEDYEKKAEFLYEELAKVRFSKHLKKVFGAG
                     YLDAKDKDDFIDKCVYLIPIIFGSGLIITTNYDKVLEKNYGLHNKTVLVSHPGHFEAL
                     NNALRNNELVLYKIHGDITEPTTSIVLTKEQYEKAYSNDELRKMLTQVYTSKELLFLG
                     CSLEKDRPLELLQQVSEAGTGHYAIIPVSSEKKKHRRIELENEYYIQTIMYPEGKHEC
                     VRIILERILEIVLPDEYRRLKDRSLKESRLTNLSCRLTEKWFQNQNNIQIKNLGDRYL
                     PELNVELSLENTFNALGKNEEFKQRFDEKTDKVLSSIKKQKINEVEVDVLVIEEIIQQ
                     FYDKANERLDIKLLKKHFEKISAALDDRINNFKNIIINEDCEKSKKREDEIYKLKQTQ
                     KDIYVYIKYLDSKEIKVVNSPYILLHSAGGMGKSHIIAHTIEKRNMDGHKSILFLGQH
                     FKLDKTPIKHMLEILEIECEFEELLEILNEIGEKEQQRVILFIDALNEGQGKELWKYY
                     LNGIVEQIKKYPWIGLILSIRSEYIPSLIDNNNKIKENLVFIEHQGFSSIEHKAMKKY
                     FDYYGIPYSNIPMPNQEFRNPLFLRIMCETFKGEEINLDTISFTDIYKNYLNILNLRI
                     SESCNYSRHANVVTEVLNEMIKYKYGDGLGNNLIPANKSVEIIINIASKYKINNVIID
                     ELIANGIVNKTVNLNDEEYMHVTYEKLEDYLYANLLVKELKEIGVVDFSHKYKYLVEY
                     EDILEVLAIVLSEETGSDIPNEIFEVFKNKETNYNVISAFINALKWRKKSTITEKTLD
                     YINKIVLGKSYYKIDFYNVLILISTKVNHTLNADYIVKYILQNTMADRDAIFIPIFNE
                     VYEEEESSIKRLLGWCFDFEGFDNTSNETIRLSAMMIATFLISSNRDLRDKSTKALVN
                     LLTGKMDIVIDLLKTFEDVDDPYIIERLYAVAFGCVVSEQSEEMIEALAVYVYNKIFD
                     VDYVYPNILLRDYAKNIVDYAKYKIDSSVLKDMNVEPPYKSIFPKVPTDEEIEGYKYD
                     YKASDFKDYYWSQNTILNSMEVEYTRDGQPGWYGDFGRYMFQHYFHKWKGLDYNDLKN
                     IAIKKVFDMGYDVNKHGKFDRTVQNGRLRDSKYERIGKKYQWIALYELAAQAADNYKI
                     EVYTDAYGTKKEIYCKGTFEPDIRNIDPTVMTVINNNNNKRVHNQLFQFSPIPNNEWL
                     GDFSDLPSINDMVNSVYKNQDFLLLNGWYTWKEENDFKNKNYKNPEKDMWVLINSYII
                     KNDQLEPIVNALKGKDFMGRWLTEPNSNYTLYNKEYYWADGYYFFQNSYYCGEEWVQI
                     DEHKNILEKDYKVLLPTYSYTTEREGDIFQEDFSGTWHKPCSTLFYGLKLRYGQGDSI
                     LYNDYGEVVCFESSELLNEEIGFFINKEIFLKYLNEQGYSVFWTMLAEKRVLEEPYMD
                     RKYIQPTISGVYTLNGENRLVGETIESEY"
BASE COUNT         5625 a         2696 c         1655 g         6604 t
ORIGIN      
        1 aaaagttcca ttaacagcat attatacgtt tttaaatatt tactagactt tttcaaaata
       61 aatatctaat tatttaaatc aataccaata taatttataa atggatttat aattgtatct
      121 tctatcatgt ttagtgtaaa caacttattt taatttaaat tcgtaaccaa caaagtacca
      181 atctgactta taattctata ttagtacaac tatggaaaaa tttaattcta tatctcctca
      241 cttaatctta tgacttctct taaatctttg taataatcct ctatattcat ttccatacat
      301 tgctttctat tttcctcagt tctactatga tcccaataaa ctccaaaatt ttgtatagtt
      361 gacactaagt ttatacattt atcaaaccaa tctttaattt caattggtgg aaaatctaaa
      421 tttataactt tattgtaaca ctcatccatt tttttctttc tactatctat aatttgaaaa
      481 atattatctt cacatatttt cttattttca tattcattat agaaggatat aatttcttca
      541 gatatgttta tcatatttgt taaaatttct ttaacttcgt atactgcctt ttctttagtg
      601 attgtatttt gacttatata tgattttaag ctttcgtagt tatcatatgt ttcagtgtga
      661 acaccgttga taaaaaattt atttttccca tttacactat accatatatc tttctcttga
      721 tatttatcaa tccaagtagt tttacacata tatattccat ttatcatatc aatttcatct
      781 ttgccaaaat atacccatat tatattagca tcttttcctt ttaatctatc ttctcctatt
      841 tcactattat aaacttcaac agacttaact atgtcaaaaa cctccttaga tatttctgat
      901 atatcacaat aggagaattt attttttaat ataacttcta ctgaatatct ttttattata
      961 cttatatgta aagatttgac attgtatatt atgtaatcgt catgctcttg aaaaactttc
     1021 ttttttatat aagatttata atcttttaca ttaaaatata tatctcccag tatgttagac
     1081 ctaaagatag gattttgttt acagtttata taatttttgt tcaatatact catacgcaaa
     1141 ttaatatact tgcttattgc tcttaaagat aactcccctt ttcttactat gtattcattt
     1201 cttaacgctt ctgataataa acttgtaaat ctactaattg aactatctgg aagagaaaat
     1261 gattcttgat attcgctact tgatactaac actgcacatc cttttctatc aaacaaggaa
     1321 aatatgtctt taatatttat tttttgtttg tcacttacat taaatttacc tgcaaagcaa
     1381 caatcaataa atacaatttt atttttacaa tttaatcctt ctataaaatc aattacaaat
     1441 tgaatagaaa ctaattcatc acttaatact aagtaatgca ctccgttatt acatatgcat
     1501 ccatgccctg aaaagtaaaa tataaatgta tcatcatctc ctatagaatc gcttatttct
     1561 tgaaaagttt taaaaaataa atcttttctg acttcatact ccatacctaa agttcttata
     1621 ttattcgaag taatccccag tcttttatgt aacacattat taatttcaat tatatcattt
     1681 ttacaaaata gtaaatcttt atttttaact atactatatt tacttactcc tacaattaat
     1741 gcttttaagc ttttcatatt ccattccccc tttataattt ttcaatttaa tatacttaaa
     1801 atgatgtttt aattgtttta ttgattttat aaaagctagt atcacactac ttttttgact
     1861 ttttcattat ttacttttgt agttggatta taatttttca aggtaagtat atatatattt
     1921 atttacatcg cacccatata atttatatct ctgatttatc tgacctgaac ttagtgtaat
     1981 ttaaaccaat gtaaaatcat ctatctcttc accatatatt ttttaaaatt ccccaaaata
     2041 ttgatgttaa tagaatttca tgtgtttttt gtattctact atccataaga attttaaagt
     2101 gagtaaatat ttccaatcat ttatatctta tttaatatca ttttattccc tcaagtattt
     2161 atttaaattg ttatgcttta tttataaaat tatacataca tttctaaagt tagacataca
     2221 tctttaaaaa cctttaattt gtgctacttt ttttctaaaa ataataaata tataaaaatt
     2281 atatatacaa aaaattaagt tatgactagt ttataaataa gtacataata aaattaaaga
     2341 gagcaatttt ttttgctctc tttcaaaatt tatattaaat tgtaataata aaaataaaaa
     2401 cgctagttat ctaaggctaa tattttcaac gattatataa atatctacat aacagttaaa
     2461 ttacatactc ttcttcagtt attgcaactt cctcagtgac atatattttt tctattttat
     2521 taagataatc tactatactt aatccaatag cctttcctag cttaacagga actgcatttc
     2581 ctatctgctt gtacacctga ctaactgacc ctgtaaactc ccactcatca ggaaaacttt
     2641 gaattctagc atactctcta gttgtgaatg gtctagtctc atctggatga cacctttcag
     2701 tttgcttttg tgcaggacta catgttaaag ttaggcatgg ttcatcccaa cttattcgcc
     2761 ttgccatacc tctttttccg ccaccgctat aataactttt tcccatataa tctcttgcca
     2821 cctcatctgg caaatctatc caacacccac ctggtggtac catatctaac acttttttct
     2881 tcctctcagg atatctagct ccttccgatt ttggaacatt tttaagtgca tctcttaacg
     2941 tagcaacata ttcaaacgac ttagggaact taaaatccac atgtgacaaa tcttttcttg
     3001 ttcctattat aataactctt tccctctttt gtgcaactcc atagttcaca gcctttagta
     3061 tctcatattt tacattgtat ccaatctcgc taaatacatc tatcatagtc tttaatgttt
     3121 ttccaccatc atgagataca agacctctaa cattctctgc taaaaacacc ttaggcttta
     3181 actcttctag aattttagca tatgaataaa acaatgtccc tcttacatcc tctaacccta
     3241 gcttcttacc cgcatagcta aatgcttggc atggataacc acctgatagc aaatctatct
     3301 ctatattatt atcaatataa ttctttattc cattttccgc tattttaaca atgtcaccct
     3361 gtataacatt ccagtctggt ctatttaatt ttaatgtatc acaagcatat ttatcccatt
     3421 ctactaatcc aacctcttta aaaccagctt tttcaaggcc taatgctaat cctcctgctc
     3481 ctgcaaataa ttcaacacat gtatatgctc ttttagctag tacattactt atatctatat
     3541 gtttattatt tttatttctc tctctaggat ttaattcata ttcactgagt tctttatatg
     3601 tacagtctct atgctctata taatctttat tactgctaca aatatttgta aatttcagtt
     3661 gcttttcatt tacatcttcc aaacacataa tttcatcgaa tccaatatct aacacttcac
     3721 ataatttttc tacattactt tttataggtg taaatttttc tgataatatt gcagataact
     3781 gattcttagt tatgcccata tttaatgcca gttgattctg acttcttata tttttctctt
     3841 tcataatttt tctcactcta tttttatcaa tagttatttt tttcatttag ttcacctctt
     3901 aataaataat atacccttaa atagttaata aaatacatta actatttaag gatatattat
     3961 cataattcta atttttatgt aagtagttat aaaaacttaa aataaaatat ctgtaaacaa
     4021 cctaatatta cctcataatt ttacacatta tggaatatat tgtataatta cattaatact
     4081 ttttaggggg attattttat gactgaatta gaaaaacaag aaatactaga taaagccaaa
     4141 gattttttta caaatatttt ggttgctaat cataaaaaga atactattaa gttaaattta
     4201 aaggcattta atataaatcc ttttttagtt aaatacttag ctagtttctt aacaggatca
     4261 aataaacctg aagatattgc taaagcattg atttatccaa gagttctagg cacttcgatt
     4321 aatacgtcct tcggaactaa tttacaaaaa ttctgtatta ccactttaga tggttttgcc
     4381 tctacaactt ctggcattga tatagagttt attgattgta ttgatggtag aaggaaattc
     4441 tgtcaaatta aagctggacc aaacactatt aataaagacg atgttaaaac tataattgac
     4501 cactttagct cagttaaaag acttgctaga acaaatggac tttcattatc ttataatgac
     4561 ttaattgttg gtgtttttta tggaacagaa aatgagctta atgctcacta taaaagaatt
     4621 aatgaagagt atccagtagt tgttggtcaa gatttttggt ataggcttac tggtgatgaa
     4681 aatttttatt ttgatttaat agatacaatt gctcaatctg ctttagaaga ggactgtact
     4741 gttttactta aaaaaacaat atccaatcta gctgatgaaa tttctaaaga taaatcttta
     4801 ttcaatcaaa agtaatataa aaatgattaa atagcataat ttacattttt agatatttaa
     4861 aaataatatt caccaaacaa aattattaag taaaatgaaa tgtatcttat aaaagctgag
     4921 ctataagtaa atctttgtaa aaacaaattt acttatagct tttttatgtt caaaaaacac
     4981 aaattatata ctatttaata tattataaac ttttatttgt taatactctc tactaaattt
     5041 ctaacatctc ttatataaat taactattta ataattcaat cataaatcta gctcctgcct
     5101 taaaaccaag cttataacca agctctcttt caatctctat aactgaatta aataaatcat
     5161 ttaactctat caatacatcc attttctttt cttctgataa atttttttct agtaatttat
     5221 taactttctt aatttcttta catctatcat tataatactc atctttttct agtactttcc
     5281 tatacatgtg ttcatttatc caataatcaa aatatatttc tataatttca tcatacatgt
     5341 tagtacctcc ttgactttca tctggtactt aatatatact taacttgtgt agattagtgt
     5401 acattaagta cctaatttat aaaggtagtt gttatattta tatggcaact acctttttct
     5461 aataaatttt tcaatctaaa tactatatat tttaaatctt cattgcttac tattgaggat
     5521 tatatacttt tcttctaaca ctccttaaac aatagtttat atatcactta ataaattata
     5581 ttttttcata cctcctttac cttcttacat tccttaaaag aaaacaaact tcctatatac
     5641 caataaggtc taaagcctga ttccccatct ctatttttag caactataat ttcacttaaa
     5701 ttagctccaa aacctttttc tctacgtctt aattctttta tatcctctat tcccattgtc
     5761 ttacaataat caagagcctc tttctctgta ggtctatgaa tatatatgac tgaattagaa
     5821 tccatatata tctgttttga acttctcatt actctatctc cttttggcct ttcatccttc
     5881 aattcatcat ttaattgagc caactgtata attggaatat taaagtctaa agtaatcttt
     5941 tttatttgcc ttgataaatc agcaaactta gcttctccaa cttctttacc ttcgtaatcc
     6001 aaaatctgaa gataatcaat tataacaaca tctggtttaa tactttttat ttctctctta
     6061 attcctgata ttgtattgac tgaatcatta atatagagat tattttcctg atttaaagta
     6121 cttaaagcat ttatgtattt aattatttct tcttcattta gatttctatt cttcattttt
     6181 acaactgata ttccagttaa agaagctatg tttctaaaca aaatttgtat atcactcatt
     6241 tccccactta taaaaagtac ttttttacct tgaaatacca catttctcat tatctgtaag
     6301 gctagtgctg tttttcctct tccacttttg gctgctattg taattaattc accactatat
     6361 agacctccaa tagtttcatc taaaaatttt attcctatat cgataccttc ttcaatttca
     6421 tcatttatgt attcaaaggc tctttctgat atagctgtta tactagaatc cttcttaacc
     6481 aatttctgat taacttcatt aacgtatttt tctaattgat atataagtgt attatcatct
     6541 atttcaccta atttgaattt attaaattta tcaattatat ttctattttt atatttttca
     6601 ataactatct caatataatg ggatacatta cctatagttg gaactattgt tgatagtttt
     6661 gtcaaataac ttatacctcc aacataatct ataagcccct ttttctttaa atcttctgta
     6721 agagttatta tatctatagg ctcatccctt ttatgtatat ctttcactga attgaatata
     6781 tgtttgtgct cttgatgata aaacatatct tcacttatta atatctcaat ctcttctatt
     6841 ttcctagaat ctagtaaaat actacctaat atagattgtt ctgcttctgt gttcttaact
     6901 aaattactca tattatccat aatataaact ccctaccact ttaattgtga atcatctgga
     6961 ctatatgctt ctgcatctac tttgacatta attgatttat cttgataatt gccttctaat
     7021 atcttaataa aattatttgg gctcattatc caatcaaaat ttgccttcca acctttttca
     7081 ttgcttccat ttaaaaagct agttttacta actatatgaa taatttctaa tacagtctct
     7141 ttatcatatt cttttattct agcattaatt actttttttc tagcttctgt aagtttcttt
     7201 acttttgata gttctgtaca agtaatattg aatttttcta taatttcact gtatatttta
     7261 cttttttcat tatttacatt cttgttagtt gttagttgct tgttaattgc atgttgatta
     7321 gtctgttgat tttcttcatt agattgatat agctcccagt tttcaatagt tataagccta
     7381 tttttgtttg ttgatttatt tgttagaaat ccatatcttt caaatttctt taaagcagtt
     7441 cttacattct gaatacttac atcttttgaa cacatatttg ctatactttc caaagatgtt
     7501 attaactgtc ctgatttaac ttcatattct ttacctccat aaacccattt attaggctta
     7561 tgattaactg acagtaatag gtttatgagt atatctcttt gtttggaatt acaatcttta
     7621 tatagtgggc tttcttttat ctttctatga agctttatcc aacctagatt actcaaatac
     7681 tatcacccac ttaagtctat aattttatac catttgcatt ttcaataaac caattatcta
     7741 gtttattttt tattatatac actctattat ctatccatat acaaggaaat cctttatttt
     7801 tagaatgaca taactgtcta aattttgatt cccctatggg ataaatttta gaagcttctt
     7861 tacaagtgat aagttgagct ttatctcttc tatatgtctg tatctccttg tacccttttt
     7921 ccttttcttg tgcttcttgt tcttgcttga caagtatatt caatgtagct tgtagtgttt
     7981 taagaacttc tagattattt gtatctgcca tacatcattc ctccttatca aaaaataatt
     8041 cttctacact tgtatctagt tcttgagcca attttaacat caacatatta cttggatttt
     8101 ttgatttacc tctttctaaa gaagataaat aatatcttga gatgccaact ttttcagcta
     8161 attcatattg ctttatacct ttttcaattc tttttaattt gagtttcatt gatttcctcc
     8221 tttctttcat acccattttg tgtatcctca tttctataaa atattctaac acccatttgg
     8281 tgtattgtca atatatttaa gtgtatcaat agacccattt agtgttataa tgtatttgag
     8341 gtgaaattat gatagataat acaattggaa aaaagattaa acaatataga aaatctataa
     8401 atatgtcaag ggatgagtta gctaataaga ttggttgctc agtttatgct gtagctaaat
     8461 atgagcaagg ccaaagaaag cctagtttag atatgataaa aaaaatatct actgcattat
     8521 caattcctat aaataggctt attattgatg aaagtgattt agatacttca acaaataatt
     8581 atactaattt aaaagaattg gatttagttg taaaaacatt tgataattac tcagcaaaac
     8641 tcatcggaaa taatataagc aaatttagaa atataaataa tatatctctc aaagaactct
     8701 cagaaaaaat aaattgttct gagaatgaaa ttataaatta tgagaatgga acaaaaatgc
     8761 ctagaattga aatattaaaa aaaatagcac ttgctactta tactaagatt gaagacttat
     8821 ttggatttcc attagaagag ataaaaatag ataaccaaac aatcctctat agaaatcccc
     8881 agactggata tattatagat aactttctta tcgattttat aaataaagat attaacatag
     8941 ataatactcc agtttcttat tatatagata agtcagaatt tgatttagta attcatatat
     9001 taaaaaatta tggttatgat gtaaaaacag attataagac aaagtttgaa attgcagata
     9061 atgcagatac aataaaacca attagcttaa gcttctctga cttcaaagac ttctccaaaa
     9121 atctaaattg ggtgataaat ggtcttgttg aaaattttat acaagaaaac tctaatggtc
     9181 ttattgaaga tgaggatgaa gaatattagt attgcacatt ttcaaaatat gagtttacct
     9241 attaactttt atacattcat agttaataaa taaaaattag atatgatatg aaaggagcta
     9301 aaatcatgaa taaatcatat ataccaaaat acacacataa attccctact aatctacctc
     9361 atggagataa aaaatatact ttaacataca ttagaagaat gataagtgaa tttgtatatg
     9421 atatgggtaa cctagaaaat aaccccttta cattcccaga agtacaaaca ttacttgatg
     9481 gtataaccgt aggtggacat aaattaagtg accaaaatca aatactaaat ataaaatcat
     9541 catgggatta tgtaataaaa ttaagtaaca aagaaaactt atctttaaat aaagatacaa
     9601 tttgctctat acataaaata gttgcaaaag atgaagctct aatagttgga gattttagaa
     9661 atggtaacat aggtatagct ggtactactc aatatgaatg catagaagcc actctattaa
     9721 atgatttatt tgtaagcgac ataaatatca taaataaaat aactaacccc ttagaaaagg
     9781 ctataataat aaatttatgg ttgtcctatt gccaattctt ctatgatggc aacaaaagaa
     9841 ctgctagact tacctcaaac ctaatactta taagtaatga tataggagtt ctatcaatac
     9901 cagctaaaca taaacaagaa tacaacacat taatgctaaa cttttatgaa acactagaag
     9961 ctgatgaagt tataaaattc ttattagaga aatgtattac attctttgat gaatataact
    10021 ataaatcata taaggagctg tttaaaaatg gcaactaaaa gagctaatgg agaaggctct
    10081 atcggaaaat ataaagatgg ttggcgttcc cgtataatga ttggctataa tgaagatggt
    10141 aaaccaataa gaaaagaatt ttatggtaag actcaaaagg aagttaaaga taaacttgat
    10201 gcttataaaa aacaatatta tttaagctct gatatttcag atgataaaat aactttagag
    10261 cattggtttt acaattggtt atttgaatat agaataaaag atttaaaacc taaatcattt
    10321 gaaagatatg aaagcatcta tcgtaattat attaaagata ctgatttagg caatgttaaa
    10381 ttaaaggact taagagtatc tcacatacaa aaatattaca atagattgct tgattccaat
    10441 aaaagtatac ctactataag gcaaataaac actaaactga aaacctgctt atcagaagct
    10501 gaaaaacaag gtcttataca acgtaattat tgtactatgg taaatatacc agttgagaaa
    10561 aaggagaata agcttgaaat attgactctt gaacaacaaa agagttttat aaaagctata
    10621 gatggacata aattagaggt attattttta cttgctcttg gtactggact tagattagga
    10681 gaattattag gacttaaatg gtttgatatt gattttaaaa agtctaattt aacagttaaa
    10741 agaacccttc aaagaacata ttttatagat aaaaccggaa atagagagct taaagtatta
    10801 gagcaagagc caaagacatc aaattcttac agaactgtac ctattccaaa agatgtttta
    10861 aataaattga aagagcataa agtaaatcaa aattcggata tattaaaggc tggagaatta
    10921 tatagaaatg ataattatgt attttgcaat gaattaggtg cccctattga tgataaaaga
    10981 cctttaagga atctaaaatc tatattaaac agcttagata tagagcctat aaaatttcat
    11041 ggtcttagga aaacttatgc gactagactt tttgagaatg atgtccctcc taaaactgta
    11101 caagttttaa tgggtcatta tgatatatca ataactttaa atatatatac tcaagttatg
    11161 gaagataaga aaattgaagc tgtagaaaag ttggataaaa tattttcatt gtaaaaatca
    11221 aatatataaa aatactctga tttcatatag ttaattacaa aaccaaaata aggatacaaa
    11281 ttaattttat attatcttgt atccttattc ttgtctagtt ttttaaatat ttatcttgga
    11341 tagcctttat gatacattaa tccattttct aacattagtt cttgggctaa atatatatga
    11401 cccttacagt gcatatctga tataccctca cttataagtt tatatgtttc agaagtatat
    11461 aaataatcca tactttcttc caagctctta cctgtttgtt tagaaaattc taatatgatt
    11521 tttttatata gcgattgtaa caatgtctta ttagcttcca taaaagctat acctcctctt
    11581 tcaaatattt gtttatccta ttttgactaa ctatatattt attagttatt taattagcca
    11641 taataattcg gctataagat atttataatc taatctcaat tatataaatc tttttgccaa
    11701 tatttaataa tcaaaatatt gcacttaatt aactatctta taagtattga taataaaatc
    11761 tgtcttctta aatatactta aataaaactt ctataattat atcaaagtat taatattcac
    11821 tctctattgt ctctcctaca agtctatttt ctccatttag ggtatatact cctgatatag
    11881 ttggttgtat gtattttcta tccatatatg gttcttctaa aactcttttt tctgctagca
    11941 tcgtccaaaa tacactatat ccttgttcat taagatattt taagaaaatt tccttattaa
    12001 taaagaatcc tatctcttca ttaagtaatt cactactttc aaaacaaaca acttcaccat
    12061 aatcattata tagaatcgaa tctccctgac catatctcaa tttcaaccca taaaatagag
    12121 tactacaagg tttatgccat gtgccagaaa agtcttcttg aaatatgtca ccttctcgtt
    12181 cagtagtata actataagtt ggcaataata ctttataatc cttttctaag atattcttat
    12241 gttcatctat ttgtacccac tcttctccac aataatatga attttgaaaa aagtaatatc
    12301 catctgccca ataatattcc ttattataaa gagtataatt actattaggt tcagtaagcc
    12361 atctacccat aaagtctttt ccctttagag cattcactat tggttctagt tggtcatttt
    12421 taattatata gctattgatt aatacccaca tgtctttttc aggattttta tagttcttat
    12481 ttttaaaatc attttcctcc ttccaagtat accatccatt aagtaataaa aagtcttgat
    12541 ttttgtaaac actatttacc atatcattta tgcttggtaa atcactaaaa tctcctaacc
    12601 actcattatt aggtattgga gaaaattgaa ataattgatt atgtactctt ttattgttat
    12661 tattatttat tactgtcatg acagttggat caatattcct aatatctggc tcaaatgtac
    12721 ccttacaata aatttctttt ttagtaccat aagcatctgt atacacttct atcttataat
    12781 tatcagcagc ttgcgctgca agttcatata atgcaatcca ttgatatttc tttcctattc
    12841 tttcatattt actatctctt aatctaccat tttgtactgt tctatcaaat ttaccatgtt
    12901 tatttacatc atatcccata tcaaaaactt tcttaatagc tatattcttt aaatcgttat
    12961 agtctagacc tttccattta tggaaatagt gttggaacat atatcttcca aaatctccat
    13021 accatcctgg ttgtccatct ctagtatatt ctacttccat agaatttaag atagtgtttt
    13081 gactccaata ataatcttta aaatctgatg ccttatagtc atacttatat ccctcaattt
    13141 cttcatcagt tggtacttta ggaaaaattg atttatatgg tggttcaaca ttcatatctt
    13201 ttaatactga actatcaatt ttatattttg cataatctac aatatttttt gcataatctc
    13261 ttaataaaat attgggataa acataatcta catcaaatat tttattataa acataaactg
    13321 ccaatgcctc tatcatctct tctgattgct ctgatacaac acatccaaat gcaactgcat
    13381 ataatcgttc aataatatat gggtcatcta catcttcaaa tgttttaagt aaatctatta
    13441 caatatccat tttccctgtt aatagattta caagagcttt tgtactttta tctcttaagt
    13501 ctctatttga agaaattaaa aacgttgcaa tcatcatagc tgacaatcta attgtttcat
    13561 ttgaagtatt atcaaaacct tcaaaatcaa agcaccatcc taataatcgc ttaatagaag
    13621 actcttcttc ttcataaact tcattaaata taggtataaa gatagcatct ctatctgcca
    13681 tagtattttg taaaatatat tttactatat aatctgcatt taatgtatga ttaacctttg
    13741 tagaaattaa gataagtaca ttataaaaat ctattttata ataagattta cctaaaacta
    13801 ttttattaat ataatctagt gttttttcag taattgtaga tttcttacgc cattttagtg
    13861 cattgataaa tgcactaata acattataat ttgtttcttt atttttaaaa acttcaaaaa
    13921 tttcattagg tatatctgag cctgtctctt cagataaaac aattgccaat acttctaata
    13981 tatcctcata ttctactaaa tatttatact tatgtgaaaa atctactacc ccaatttctt
    14041 ttaactcttt aaccaacaaa tttgcatata aataatcttc taacttctca tatgtcacat
    14101 gcatatattc ttcatcattt aaattgactg ttttattaac tattccattt gctatgagtt
    14161 catctattat cacattgtta atcttatact tacttgcaat atttattata atctctacac
    14221 tcttgtttgc gggtattaaa ttattcccaa gaccatctcc atatttatat ttaatcattt
    14281 cattaagtac ttctgtaact acattggcat gtctactata attgcatgat tcagatattc
    14341 taagattaag tatattcaaa taatttttat aaatatctgt aaaactaata gtatctaagt
    14401 taatctcttc tcctttaaaa gtttcacaca taattctcaa aaaaagaggg tttctaaatt
    14461 cttgatttgg cattggaata ttagaataag gtattccata ataatcaaaa tactttttca
    14521 tagctttatg ttctatacta gaaaatcctt gatgctctat aaaaaccaaa ttctctttta
    14581 tcttgttatt attatctatt aaagatggta tatattcact tcttattgat aatattagtc
    14641 caatccatgg atatttcttt atctgttcta caataccatt taaataatat ttccaaagtt
    14701 cttttccttg accttcattc aatgcatcta taaataatat cacacgctgt tgctcttttt
    14761 ctcctatttc attaagtatt tctaataatt cttcaaactc acactcaatt tctaatattt
    14821 ctaacatatg tttaattggt gttttatcca acttaaaatg ttgccctaaa aataatatag
    14881 acttatgccc atccatattt cttttctcaa tagtatgtgc aattatatga gatttcccca
    14941 taccacctgc actatgtaat aaaatataag ggctattaac tacttttatt tccttagaat
    15001 ccaaatattt tatatataca tagatatctt tttgagtctg cttcaatttg tatatttcat
    15061 cttctctttt tttacttttt tcacagtctt catttataat tatattttta aaattattta
    15121 ttctatcatc aagtgctgca cttatttttt caaagtgctt ttttaacaac ttgatatcta
    15181 atctttcatt agctttgtca taaaattgtt gtattatttc ttctataact agtacatcta
    15241 cttccacttc atttattttc tgtttcttta tacttgatag aactttatct gttttttcat
    15301 caaaacgttg tttaaattct tcattttttc ctaatgcatt aaacgtattt tctaaactca
    15361 attctacatt caattctggt aaatatctat ctccaaggtt ttttatctgt atattattct
    15421 gattttgaaa ccatttctct gttaatctac aagataaatt agttaaccta ctttccttta
    15481 aagacctatc ctttaatctt ctatattcat ctggcaaaac tatttccaaa attcgttcaa
    15541 gtattattct tacacactca tgcttacctt caggatacat aatggtttgt atataatact
    15601 cattttctaa ctctattcgt ctatgtttct ttttctcact actaactgga ataatagcat
    15661 aatgacccgt tcctgcctct gatacttgct gtaataattc caacggtcta tccttttcca
    15721 aactacatcc aagaaataac aactctttac ttgtatatac ttgtgtaagc atctttctca
    15781 attcatcatt actatatgct ttttcatatt gttcttttgt taacacaata gatgttgtag
    15841 gctctgtaat atccccatga attttataca atactaattc attatttcta agtgcattat
    15901 ttaatgcttc aaagtgacct ggatgtgaca ctaaaactgt tttattgtgt aatccataat
    15961 ttttttctag tactttatca taattagtag ttataattaa tcctgagcca aaaattatag
    16021 gaattaaata gacacattta tcaataaaat cgtctttatc ttttgcatcc agataacctg
    16081 ccccaaaaac tttttttaaa tgtttagaaa atctaacttt cgccaattct tcatataaaa
    16141 attctgcttt tttctcataa tcctctattt tttcaaataa aattttatcc tcatcttcta
    16201 aatattcttc acttgtgtta tttaaaaatg tactccatga aggatatatg tctttaaata
    16261 acatagacat gcctgctcca atatatggga ctacataacc atctttaatt gcatttacca
    16321 gttcatcaaa atttttttga ttgtaaggct tattaaattc cataatttct tcaaatgtca
    16381 ttattccctc ctaaactatc tcttaataat ttcaaccata attcctattt tataatttta
    16441 ttatatcatt ccattccatt tttttactac aattctatgt tatagaatga attcacttac
    16501 ttacatttgt taataatata aaactttctt ttaagataat taaaatttat acttttaaat
    16561 tatggataaa aatggaatgt
//
LOCUS       DAMJYR010000085        15978 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.087, whole genome shotgun sequence.
ACCESSION   DAMJYR010000085 DAMJYR010000000
VERSION     DAMJYR010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15978)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15978)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15978
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.087"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            248..397
                     /gene="rpmG"
                     /locus_tag="PC471_002610"
     CDS             248..397
                     /gene="rpmG"
                     /locus_tag="PC471_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004402360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HDF2789728.1"
                     /translation="MRVKVTLACTECKQRNYNTTKNKKNNPDRIELQKYCRFCKKHTT
                     HKETK"
     gene            423..644
                     /gene="secE"
                     /locus_tag="PC471_002611"
     CDS             423..644
                     /gene="secE"
                     /locus_tag="PC471_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HDF2789729.1"
                     /translation="MAAQKNDGAKTKKRFSLFGYLKETKQELKRVTWPTKKELFKNTS
                     IVLTVVISCTILVWGIDTILSGALALLLK"
     gene            664..1206
                     /gene="nusG"
                     /locus_tag="PC471_002612"
     CDS             664..1206
                     /gene="nusG"
                     /locus_tag="PC471_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination factor
                     NusG"
                     /protein_id="HDF2789730.1"
                     /translation="MSELQEASWYVVHTYSGHENKVKATIEKAVKTRGMEDCIRQVVV
                     PTEEVVETTKTGKEKTRQRKVYPSYVLVKMIITDESWYVVRNTKGVTGFVGPGSKPVP
                     LSEDEVKAMGIDTTDPKVVNSDIDFEIGDTVKVSQGPFSGQIGNIEEIDLENREVKVC
                     INAFGKRTLFVIELEGIEKI"
     gene            1245..1670
                     /gene="rplK"
                     /locus_tag="PC471_002613"
     CDS             1245..1670
                     /gene="rplK"
                     /locus_tag="PC471_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012158357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HDF2789731.1"
                     /translation="MAKKVIGQIKLQIPAGKATPAPPVGPALGQHGVNIMGFTKEFNA
                     KTADQAGMIIPVVITVYQDRSFSFITKTPPAAVLIKKALNLKSGSGEPNKKKVAKMTS
                     AQVREIAELKMPDLNAASVEAAMSMIAGTARSMGVVIED"
     gene            1741..2439
                     /gene="rplA"
                     /locus_tag="PC471_002614"
     CDS             1741..2439
                     /gene="rplA"
                     /locus_tag="PC471_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HDF2789732.1"
                     /translation="MAKKGKRYAGALQKVDRTKFYDASEALTLVSDIAGAKFDETVEA
                     HIKLGVDSRHADQQVRGAVVLPHGTGKTKRVLVFAKGEKAKEAEQAGADFVGAEELVQ
                     KIQGENWFDFDIVVATPDMMGVVGRLGRVLGPKGLMPNPKSGTVTFDVAKAIDEIKAG
                     KVEYRLDKTNIIHVPVGKVSFGGEKLTENFTALMDAIIKAKPAAAKGQYLRSITVASS
                     MGPGVKINPARTAE"
     misc_feature    2491..2619
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            2662..3168
                     /locus_tag="PC471_002615"
     CDS             2662..3168
                     /locus_tag="PC471_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HDF2789733.1"
                     /translation="MRKAIEIKSEVVSEIVEKLQKSSAAVVVDYKGLTVEEVTELRKQ
                     MREAGVDYKVYKNTLVRRAAKEVGIEQFNDELLVGTNAIAFGYDDPVAPARILKGFMD
                     SHPKMKLKMGIVEGAFYDESKIVEMANIPSREVLIAKLLGSLKAPVSNFAYLIDAIAK
                     KAEGQEEA"
     gene            3226..3591
                     /gene="rplL"
                     /locus_tag="PC471_002616"
     CDS             3226..3591
                     /gene="rplL"
                     /locus_tag="PC471_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000159736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HDF2789734.1"
                     /translation="MTIEQILEAIENMKVLELNELVKAAEEKFGVSASAPVMVAGAAA
                     GGPAAEEKTEFDVVLTDVGSSKVGVIKAVREITGLGLKEAKEVVDNAPKTVKEGASKE
                     EADQIKEKLEAAGAKVEVK"
     gene            3982..4770
                     /locus_tag="PC471_002617"
     CDS             3982..4770
                     /locus_tag="PC471_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDF2789735.1"
                     /translation="MEKLQGKIAVVTAATKGIGLASAEILAKNGATVYLAARSEELAN
                     EVINKISAEGGCAKFVYFNAREEETFTSMIEEVVKKEGKIDILVNNFGSTNPSLDKDL
                     VTGDTDNFFDTVNTNLKSVYLPCKAAIPHMIKNGKGSIVNISSIGSVLPDLSRIAYCV
                     SKAAINSLTQNIATQYAKDNVRCNAVLPGLIATKAALDNMSPEFIKEFLKHVPLNRIG
                     EPDDIAKAVLFYASDDSSFITGDLLEVAGGFGLPTPQFADNILG"
     gene            5241..8957
                     /gene="rpoB"
                     /locus_tag="PC471_002618"
     CDS             5241..8957
                     /gene="rpoB"
                     /locus_tag="PC471_002618"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HDF2789736.1"
                     /translation="MPHPVTIGKRTRMSFSKIKEIADVPNLIEIQVDSYEWFLKEGLK
                     EVFDDISPIEDYTGNLILEFVDYSLDDKPKYDIEECKERDATYCAPLKVKVRLINKET
                     GEIKEQEVFMGDFPLMTERGTFVINGAERVIVSQLVRSPGVYYAEERDKTGKRLISST
                     VIPNRGAWLEYETDSNDVISVRVDRTRKQPVTVLLRALGIGTDAEIIDLLGEDERLSA
                     TLEKDNTKTVEEGLVEIYKKLRPGEPPTVESASSLLNALFFDPKRYDLAKVGRYKFNK
                     KLALCYRIMNKISAEDIINPETGEVFVKAGEKISYDLAKAIQNAGINVVNLLMDDDKK
                     VRVIGNNFVDIKSHIDFDIDDLNIKEKVHYPTLKEILDGYSDEEEIKEAIKSRIKELI
                     PKHILLDDIIASISYEFNIFYNIGNIDDIDHLGNRRIRSVGELLQNQVRIGLSRMERV
                     IKERMTVQDMEAITPQALVNIRPVSAAIKEFFGSSQLSQFMDQTNPLSELTHKRRLSA
                     LGPGGLSRERAGFEVRDVHHSHYGRMCPIETPEGPNIGLINSLGTYAKINEFGFIESP
                     YRKFDKETSTVTDEIHYLTADEEDLFVRAQANEPLTEDGKFVNHRVVCRTVNGAVEMV
                     PESRVDYMDISPKQVVSVATAMIPFLENDDANRALMGANMQRQAVPLVRREAPIIGTG
                     IEYRAAKDSGAVVVARNSGIAERVTADEIIIKREDGNRDRYNLLKFKRSNSGTCINQT
                     PIINKGDQIIKGDVIADGPATDLGEVALGRNCLIAFMTWEGYNYEDAILINERLVKED
                     RLSTIHIEEYECEARDTKLGPEEITRDIPNVGDSAIKNLDDRGIIRIGAEVDSGDILV
                     GKVTPKGETELTAEERLLRAIFGEKAREVRDTSLKVPHGESGIIVDVKVFTRENGDDL
                     SPGVNELVRCYIAKKRKIKVGDKMAGRHGNKGVISRVLPEEDMPFMENGTPLDIILNP
                     QGIPSRMNIGQVLEVHLGLAAKTLGWYVATSVFDGANEYDIMDALEEAGYPRDGKLTL
                     YDGRTGESFDNRITVGYMYYLKLHHLVDEKLHARSTGPYSLVTQQPLGGKAQFGGQRF
                     GEMEVWALEAYGAAHILQEILTVKSDDVVGRVRTYEAIVKGENIPEPGIPESFKVLIK
                     ELQSLCLDVKVLTDEDQEIEVRESVDEDDTIGEFELDVVNHMGEVEESNIIEEIEDDF
                     AENAEDEDIENLEEFTEDDLFEEEIDFDSDDFDM"
     gene            8999..12484
                     /gene="rpoC"
                     /locus_tag="PC471_002619"
     CDS             8999..12484
                     /gene="rpoC"
                     /locus_tag="PC471_002619"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HDF2789737.1"
                     /translation="MFELNNFESIKIALASPEKIRQWSRGEVKKPETINYRTLKPEKD
                     GLFCERIFGPQKDWECHCGKYRRVRYKGVVCDRCGVEVTKSKVRRERMGHIELAAPMS
                     HIWYFKGIPSRMGLLLDMSPRSLEKILYFASYVVVDPGETGLNEKQLLTEKEYRTALE
                     KYGYTFTVGMGAEAVKTLLQNIDLEQQSKDLRAELKDSTGQKKVRTIRRLEVVEAFKK
                     SGNKPEWMILDAIPVIPPDLRPMVQLDGGRFATSDLNDLYRRVINRNNRLKRLLELGA
                     PDIIVRNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSDMLKGKQGRFRQNLLGK
                     RVDYSGRSVIVVGPELKFYQCGLPKKMALELFKPFVMDKLVKEGYAHNIKSAKSIVEK
                     VKPEVWDVLEDVIKSHPVLLNRAPTLHRLGIQAFEPILVEGKAIKLHPLVCTAYNADF
                     DGDQMAVHVPLSVEAQAEARFLMLSVNNILAPKDGSPITTPSQDMVLGCYYLTIEAQD
                     GAKGTGMVFKDFNELLLAYYNKSVHLHALVKLKVTLEDGRSSLVESTVGRFIFNENIP
                     QDLGFVDRKENPFALEVDFLADKKSLGKIIDKCFRKHGNTETAELLDYIKALGFKYST
                     LGGITVAVDDMSVPEEKKVFIAEAEAKVDKYEKAYRRGLISDEERYEKVIETWTETTD
                     KVTDALMGGLDRLNNIYIMAHSGARGSKNQIRQLAGMRGLMANASGKTVEIPVKSNFR
                     EGLSVLEYFTSSHGARKGLADTAIRTAESGYLTRRLVDVSQDVIVREIDCGTEDTTEI
                     YAIKEGNEVIEEIYDRIVGRYTIDPILNPETGEVIVEADSMIQEDEAETIVALGIEKI
                     RIRTVLNCKTNHGVCSKCYGRNLATGKEVNIGEAVGIIAAQSIGEPGTQLTMRTFHTG
                     GVAGADITQGLPRVEELFEARKPKGLAVITEVSGRVEIDETGKRKEVNVIPEEGETQT
                     YVIPYGSRLKVKQGQMLEAGDPLTQGFINPHDIVRVNGVKGVQEYIVKEVQRVYRLQG
                     VDVNDKHIEVIVRQMLSKVKVEDPGDTDLLPGGYEDVLTFNECNKDAIDKGLRPAVAK
                     RVLLGITKASLATDSFLSAASFQETTRVLTEAAIKGKEDHLIGLKENVILGKLIPAGT
                     GMKKYRNIAVEKIED"
     gene            12773..13195
                     /gene="rpsL"
                     /locus_tag="PC471_002620"
     CDS             12773..13195
                     /gene="rpsL"
                     /locus_tag="PC471_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001142340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HDF2789738.1"
                     /translation="MPTINQLVRKSRKALEKKSTAPALQKGYNSLNKKVTDASAPQKR
                     GVCTSVKTVTPKKPNSALRKVARVRLTNGIEVSAYIPGEGHNLQEHSVVLIRGGRVKD
                     LPGVRYHILRGTLDTAGVDKRRQSRSKYGAKRPKEAKK"
     gene            13319..13789
                     /gene="rpsG"
                     /locus_tag="PC471_002621"
     CDS             13319..13789
                     /gene="rpsG"
                     /locus_tag="PC471_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001137493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HDF2789739.1"
                     /translation="MPRKGNIPKREVLPDPMYGSKVVTKLINNLMVDGKKGKSQRIVY
                     DAFAIVAEKTGEEALEVFNKAMDNIMPVLEVKARRVGGANYQVPIEVRPERRQTLGLR
                     WLVKYTRARGEKGMVEKLAKEIMDAANNTGASVKKKEDTHKMAEANKAFAHYRW"
     gene            13838..15904
                     /gene="fusA"
                     /locus_tag="PC471_002622"
     CDS             13838..15904
                     /gene="fusA"
                     /locus_tag="PC471_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HDF2789740.1"
                     /translation="MARKFPLERTRNIGIMAHIDAGKTTTTERILFYTGQTHKIGETH
                     EGASQMDWMEQEKERGITITSAATTASWKDHRINIIDTPGHVDFTVEVERSLRVLDGS
                     VAVFCAKGGVEPQSENVWRQAETYGVPRIAFVNKMDILGADFYNVVSMMKSRLNSNAV
                     PMQLPIGKEDSFIGIIDLLKMDAVIYKDDLGVEMEETDIPEDMKELAAEWREKLVESV
                     AETDEELMMKYLEGEELTIDELKVAIRKATIACEMNPVFCGTAYRNKGVQLVIDAVLD
                     YLPAPTDIPAIKGILADGEEAERHSSDEEPFSALAFKIMTDPFVGKLAFFRVYSGTLE
                     SGSYVLNATKNKRERIGRILQMHANTREEITKVYAGDIAAAVGLKDTTTGDTLCDPAN
                     PIILESMEFPEPVISVAIEPSSKAAQEKMGIALQKLAEEDPTFTVKTDQETGQTIISG
                     MGELHLEIIVDRLLREFKVEAKVGAPQVAYRETITQPVDVEYKYSKQSGGRGQYGHVK
                     IRVAPQEPGEGYKFTNKTVGGSVPKEYVGPVDMGIQGAMQSGIVAGYPVVDVAVELYD
                     GSYHEVDSSEMAFKMAGSMAFKDAMKKGNAVLLEPYFKVEVVTPEDYMGDVMGDLNSR
                     RGLIQGMEARSGAQVINAFVPLSEMFGYSTDLRSSTQGRATYTMIFDHYEQVPASVAK
                     KIAEGK"
BASE COUNT         6257 a         1989 c         3249 g         4483 t
ORIGIN      
        1 ctttactaaa ctgatggatt aaaaggaata acaaaaagag gggttatgct cctctttttt
       61 taacatagaa atacaaaaac attgacacag cataggattt tgtgataact tatacaaggt
      121 aatgattata ggaagttaat aatatcttga atactgtaga tttttaataa atacatattt
      181 taaagatatt atattcacat atattaacaa tatatattat tgtattacaa agttggaggt
      241 gtatcagatg agagttaaag taactttagc atgtacagag tgtaagcaaa gaaactacaa
      301 cactactaag aataagaaaa ataatccaga cagaatagaa ttacaaaaat attgtagatt
      361 ctgtaaaaag catacaactc ataaagaaac aaaatagttt tttataagga tgtgagtatg
      421 gaatggctgc ccaaaaaaat gacggtgcaa aaactaaaaa aaggtttagt ttgtttggat
      481 acttaaaaga aactaaacaa gaattaaaaa gagttacatg gccgacaaaa aaggaactgt
      541 ttaaaaacac aagtatagtt ttaactgtag ttatttcatg cactatatta gtatggggta
      601 tagacactat attatctggt gcactagcgt tattactaaa atagacgaaa agaggtaagt
      661 taaatgtcag aattacaaga agcaagttgg tatgtagttc atacctattc gggacatgaa
      721 aataaagtta aagctacaat tgagaaagca gttaaaacaa gaggtatgga agactgcata
      781 agacaagtag ttgttccaac agaagaagta gtggaaacta ctaaaactgg aaaagaaaaa
      841 actagacaac gtaaagttta tccaagttat gttttggtta aaatgataat aactgatgaa
      901 tcttggtatg tagtaagaaa tactaaggga gttacaggtt tcgttggacc tggttcaaaa
      961 ccagtgccat taagtgaaga cgaagtcaaa gctatgggga tagacacaac agaccctaaa
     1021 gtagtaaata gcgatattga ttttgaaata ggagatacag taaaagtatc tcaaggacca
     1081 tttagtggac aaataggtaa catagaagaa attgacttag aaaatagaga agtcaaagta
     1141 tgcataaatg catttggtaa aaggactcta tttgtaatag agcttgaagg tatagaaaaa
     1201 atctaatact atattataga aaaatctgag gaggtgcgct caaaatggct aaaaaagtta
     1261 taggtcaaat aaaattacaa atacctgcag gaaaggctac tccagcgcca ccagttggac
     1321 cagcattagg acaacatggt gttaatataa tgggattcac aaaagaattt aatgctaaaa
     1381 ctgcagatca agctggaatg ataataccag ttgttataac tgtatatcaa gatagatcat
     1441 tcagttttat aacaaaaact ccaccagctg cagttttaat taaaaaagca ttaaacttaa
     1501 aatcaggttc tggagaacca aacaagaaaa aagttgctaa aatgacttca gctcaagtaa
     1561 gagaaatagc tgaattaaag atgcctgact taaatgctgc atcagtagaa gctgctatga
     1621 gtatgatagc tggtactgct agaagcatgg gtgttgtaat cgaagactaa ttaaaagata
     1681 cacttttttt tataaagtgg gaggctagaa gccgaaatta accacgagga ggaaaataaa
     1741 atggctaaaa aaggtaaaag atatgcaggt gcattacaaa aagtagatag aactaaattt
     1801 tatgatgcat cagaagcatt aacattagtt tcagatatag ctggagctaa atttgatgag
     1861 acagtagaag cacacattaa attaggtgtt gactcaagac atgctgacca acaagtaaga
     1921 ggtgcagttg tattacctca tggaactggt aaaacaaaaa gagttttagt ttttgctaaa
     1981 ggtgaaaaag ctaaagaagc tgaacaagct ggtgctgact ttgtaggagc tgaagaatta
     2041 gttcaaaaaa tacaaggtga aaactggttt gactttgata tagtagttgc tactccagat
     2101 atgatgggtg tagtaggtag attaggtaga gtattaggac ctaaaggttt aatgccaaac
     2161 cctaaatcag gaacagttac atttgatgta gctaaagcta tagatgaaat aaaagctggt
     2221 aaagttgaat acagattaga caaaactaac ataatacacg ttccagtagg aaaagtatca
     2281 tttggtggag aaaaattaac tgaaaacttt actgcattaa tggatgctat aataaaagct
     2341 aaaccagctg ctgctaaagg acaatattta agaagtataa cagtagcttc ttcaatggga
     2401 cctggagtta aaataaaccc agcaagaaca gctgaataat aaaactgttg acacaggttg
     2461 acaaaaatgt tattatatca cttgtcaatg taaaaaagaa tagatttgcc gtagatagtg
     2521 ggtgcgaaag cttaatatcc cgccgaggtt ttagataaat tgagattact aaagcttttg
     2581 atgtctactt ttggcaacaa aagcttttta taatgttgcg gtaaatacta agcctatttg
     2641 gtgaaggagg tgcacatcta tatgagaaaa gctatagaaa ttaaatcaga agttgtttct
     2701 gagatagttg aaaagttaca aaaatcttct gctgctgttg ttgtagatta caaaggatta
     2761 acagttgaag aagtaactga gttaagaaaa caaatgagag aagctggtgt agattacaaa
     2821 gtatacaaaa atactttagt tagaagagca gctaaagaag ttggtataga gcaatttaat
     2881 gatgaattat tagttggaac taatgccata gcatttggat atgatgatcc agttgctcca
     2941 gctagaatat taaaaggatt catggattct catccaaaaa tgaaattaaa gatgggtatt
     3001 gtggaaggtg cattctatga tgaatctaag attgttgaaa tggctaacat accatcaaga
     3061 gaagttctta tcgctaaatt acttggtagc ttaaaagctc cagtatcaaa cttcgcatac
     3121 ttaatagatg caatagcgaa aaaggcagaa ggtcaagaag aagcttaatt gcttaactaa
     3181 aaaattataa atattaattt gaaataaaat tcggaggtgc taaaaatgac aatagaacaa
     3241 atattagaag ctatagaaaa tatgaaagtt ttagaattaa atgaattagt taaagctgct
     3301 gaagaaaaat tcggtgtatc agcttcagct ccagtaatgg tagctggtgc agctgctggt
     3361 ggaccagctg ctgaagaaaa aactgagttt gatgtagtat taactgacgt tggttcttca
     3421 aaagttggag ttataaaagc agttagagaa ataacaggat taggattaaa agaagctaaa
     3481 gaagtagttg acaatgctcc taagacagtt aaagaaggag cttctaaaga agaagctgat
     3541 caaataaaag agaaattaga agctgctgga gctaaagtag aagttaagta gtttctttaa
     3601 aaagataccc attgggtatc tttttttatt tataaaaaac tataaagaaa gtatttggaa
     3661 ttataaagaa agtattttta taaaataaaa aaaatttata taatagtact aaattaaggg
     3721 tatcatatat ggtgtaatgg tattttaatt taacattatt atcaaattaa aatataaaca
     3781 attgttgtaa tgtctgtata taaagtatat aattaaacta aaatgtaaaa ttttacattt
     3841 tagtttaaaa aaatacacga gtgtttataa ataaaatgta ataaataatt aattaacaaa
     3901 tataaatttt aaaataagtt agatatttag aaaaatcgaa atttgtagga tttaaaggac
     3961 atattcaagg aggagttaat aatggaaaaa ttacaaggaa aaattgcagt agttactgca
     4021 gcaacaaaag gtattggatt agcatcagca gagatattag caaaaaatgg agcaactgtg
     4081 tacttagcag ctcgttcaga agaattagct aatgaagtta taaataaaat aagtgcagaa
     4141 ggtggttgtg ctaagtttgt ttactttaat gctcgtgaag aagagacctt tacctcaatg
     4201 atagaagagg tagttaaaaa agaaggtaag atagatatac ttgtaaacaa ttttggttca
     4261 acaaaccctt ctcttgacaa agaccttgta actggggata cagataattt ctttgacaca
     4321 gtaaatacta atttaaaaag tgtgtattta ccatgtaaag cagcaattcc tcatatgata
     4381 aagaatggaa aaggaagtat agtaaatata tcaagtatag gttcagtatt acctgattta
     4441 tctagaatag cttactgtgt atcaaaagca gcaatcaact cattaactca aaacatagct
     4501 acacaatatg ctaaagacaa tgttagatgt aatgctgtac ttccagggct tattgcaact
     4561 aaagcagcat tagacaatat gtctccagag ttcataaaag aatttttaaa gcatgttcct
     4621 ttaaatcgta taggggaacc agatgatata gcaaaagctg ttctattcta tgctagtgat
     4681 gactcatcat ttataacagg ggatttactt gaagttgcag gaggatttgg tttacctact
     4741 ccacaatttg cagataatat attaggataa ttaaattact aatatgcaat gaaagcttat
     4801 gcattagtta tagtagaaat gatattttta taaaggccat agatataatt tatattggaa
     4861 aggtttgagg ttcaaaattc caagttaaat ttatttatgg tctttatttt gtatatttta
     4921 ataaattgta tatatttgta taaatgtaat tatttacaat cattttaata tggaattata
     4981 attaaatagg ggggatggta cttgtataaa attttttata gaaaattaga cctataaatg
     5041 aaaaatatgg attttatatt ggaaaattgt acataataca taatatatgc ttaaaataat
     5101 ttacaaaaaa tcaaaaataa tgcatagttt acatataaga taaaaaatat attgaaaaat
     5161 acttgacatt atgctaaaat tattagatgc gttagaatga actattgttt tatttttaat
     5221 tattgagagg tgaaaagtga atgccacatc ctgtcacgat aggtaaaaga actagaatga
     5281 gcttttctaa aattaaggaa atagctgatg tgccaaatct tatagaaatt caagtagatt
     5341 cctatgagtg gtttttaaaa gaaggtttaa aagaagtgtt tgatgatatt tcaccaatag
     5401 aagattatac tggtaatctt atattggaat ttgtcgacta ttctctagat gataaaccaa
     5461 agtacgatat agaagagtgt aaagagagag atgctacata ttgtgcacct ctaaaggtta
     5521 aggtaagact tataaataaa gaaacaggtg agataaaaga acaagaagtg tttatgggtg
     5581 acttcccttt aatgacagaa agaggaactt tcgttataaa tggagcagaa agagttatag
     5641 taagtcaatt agttagatct cctggtgttt actatgcgga agaaagagat aaaacaggta
     5701 agagattgat ttcatctact gtaattccta atagaggagc atggttagaa tatgaaacag
     5761 attctaatga tgtaatatct gtaagagttg atagaacaag aaaacaacca gttacagttt
     5821 tacttagagc tttaggaata ggaacagatg cagagataat tgacctttta ggagaagatg
     5881 agagattatc tgctacatta gaaaaagata atactaagac agtagaagaa ggtcttgtag
     5941 agatatacaa aaaattaaga ccaggtgaac ctcctactgt agaaagtgca tcatctttat
     6001 taaatgcttt attctttgac ccaaaaagat atgacctagc aaaagttggt agatataaat
     6061 tcaataagaa acttgcttta tgttatagaa taatgaataa aatttcagca gaagacataa
     6121 taaatccaga aactggagaa gtatttgtaa aagctggaga aaaaataagc tatgatttgg
     6181 ctaaagccat acaaaatgca ggtataaatg tagtaaatct actaatggat gatgataaaa
     6241 aagttagagt tataggaaat aattttgtgg atattaagtc tcacattgat tttgacatag
     6301 atgatttaaa cataaaagaa aaagtacatt atccaacttt aaaagaaatt ttagatggat
     6361 atagtgatga agaagaaata aaagaggcta taaaatcaag aattaaagaa cttataccaa
     6421 aacatatatt attagatgat ataatagctt caataagtta tgagttcaat atattctata
     6481 atataggaaa tattgatgat atagatcacc taggaaacag aagaataaga tctgtaggtg
     6541 aattgttaca aaatcaagtt agaattggtc tttcaagaat ggaaagagtt ataaaagaaa
     6601 gaatgacggt tcaagatatg gaagctataa cgcctcaagc gttagttaac ataagaccag
     6661 tttcagctgc aataaaagaa ttttttggta gttctcagtt atctcagttc atggatcaaa
     6721 caaatcctct atctgagtta acgcataaga gaagattatc agcccttggg cctggaggtc
     6781 tttcaagaga aagagctgga ttcgaagtgc gtgacgttca ccattcacac tatggtagaa
     6841 tgtgtccaat agagactcca gagggaccaa atatcggtct tataaactct ctaggaactt
     6901 atgcaaaaat aaatgaattt ggatttatag aatcaccata tagaaaattt gataaagaaa
     6961 catcaacagt tactgatgaa atacattatt taactgctga cgaagaagat ttattcgtaa
     7021 gagctcaggc aaatgagcca ttaacagaag atggtaaatt cgtaaaccat agagttgttt
     7081 gtagaactgt aaatggtgct gttgaaatgg ttcctgaaag tagagtagat tacatggata
     7141 tatctcctaa gcaggttgta tctgttgcca ctgctatgat acctttctta gaaaatgatg
     7201 atgccaaccg tgcccttatg ggagcgaaca tgcagcgtca ggcagtgcct ctagttagaa
     7261 gagaagcacc aattatagga actggtatag aatacagagc agcaaaagac tctggagcag
     7321 ttgttgttgc tagaaattct ggtatagcag aaagagtaac agcagatgaa ataattataa
     7381 aaagagaaga tggaaacaga gatagatata atctacttaa gtttaaacgt tcaaactcag
     7441 gtacttgtat aaatcaaaca cctataataa ataagggtga ccagataata aaaggtgatg
     7501 ttatagcaga tggtccagca actgatttag gagaagttgc acttggaaga aactgtctta
     7561 tagcattcat gacttgggaa ggttataact acgaggatgc tatattaata aatgaaagat
     7621 tagttaaaga agatagatta tcaacaattc atatagaaga atatgaatgt gaagctagag
     7681 atacaaaact agggccagag gaaataacta gagatatacc taatgttgga gatagtgcaa
     7741 ttaagaactt agatgacaga ggtataataa gaataggtgc agaagtagac tcaggtgata
     7801 tattagttgg taaagtaact ccaaaaggag aaactgaact tactgctgag gaaagattac
     7861 ttcgtgcaat atttggagaa aaggctagag aagttagaga tacttcactt aaagtgcctc
     7921 atggtgaatc tggtataata gttgatgtaa aagtatttac tagagaaaat ggagatgatt
     7981 tatctccagg agtaaatgaa ttagtaagat gctatatagc taaaaagaga aaaataaaag
     8041 ttggagataa aatggctggt cgtcatggta ataaaggggt tatctcaaga gtattacctg
     8101 aagaagatat gccattcatg gaaaatggaa caccactaga cataatactt aacccacaag
     8161 gtataccatc acgtatgaac attggtcagg ttttagaagt tcatttagga cttgctgcta
     8221 agacattagg atggtatgta gcaacatcag tattcgatgg tgctaatgag tatgacataa
     8281 tggatgctct tgaagaagct gggtatccta gagatggtaa attaacttta tatgatggta
     8341 gaacaggtga gtcttttgac aacagaataa ctgttggata tatgtactac ttgaaactac
     8401 atcacttagt agatgaaaaa ctacatgcaa gaagtactgg accatactca ttagttactc
     8461 aacagccact aggtggtaaa gcacaatttg gtggacaaag atttggagag atggaggttt
     8521 gggctctgga agcatatgga gctgctcaca tacttcaaga aatacttact gttaagtctg
     8581 atgacgttgt aggtcgtgtt agaacatatg aagctatagt taaaggtgaa aatatacctg
     8641 aaccaggaat cccagaatca tttaaggttc ttataaaaga acttcaaagt ttatgcttag
     8701 atgtaaaagt attgacagat gaagaccaag aaatagaagt aagagaatct gtggatgaag
     8761 atgatacaat aggtgaattt gagctagatg ttgtaaatca tatgggagaa gttgaagaaa
     8821 gtaatataat agaagaaatt gaagatgatt ttgcagaaaa tgcagaagat gaggatatag
     8881 agaatttaga agaattcact gaagatgatt tatttgaaga agaaatagat tttgatagtg
     8941 atgactttga tatgtaaatt taattaatgt aaatctcaaa tagaagggag agaactcctt
     9001 gtttgaatta aacaatttcg agtcgataaa aatagcattg gcttctccag aaaaaataag
     9061 acaatggtct aggggagaag ttaaaaagcc agaaactata aattaccgta ctttaaaacc
     9121 agaaaaagat ggtcttttct gtgaaagaat atttggacca caaaaagact gggagtgcca
     9181 ctgtggtaaa tatagaagag ttagatataa aggtgtagtt tgtgatagat gtggagtaga
     9241 agtaactaaa tcaaaagtaa gaagagagag aatgggacat atagagctag ctgctcctat
     9301 gtctcacatc tggtacttca aaggtatacc aagtagaatg ggacttttac ttgatatgtc
     9361 accaagatcg ttagaaaaaa tattatactt tgcctcatat gtggtagttg atccaggaga
     9421 gactggatta aacgaaaaac aattgcttac agaaaaagaa tacagaactg ctcttgaaaa
     9481 gtatggatat acttttactg taggaatggg tgctgaagct gtaaaaacat tactacaaaa
     9541 tatagattta gagcaacaaa gtaaagacct aagagcggag ttaaaagata gtacagggca
     9601 aaagaaagtt agaacaataa gaagattaga agttgtagag gcatttaaaa agtctggaaa
     9661 taaaccagaa tggatgattt tagatgcaat accagtaata ccaccagatt taagaccaat
     9721 ggtacaactt gatggtggaa gatttgcgac ttcagaccta aatgatttat atagaagagt
     9781 tataaataga aacaacagac ttaaaagatt attagagctt ggagctccag atataattgt
     9841 aagaaatgaa aaaagaatgc ttcaagaagc tgttgatgca ttgatagata atggtagaag
     9901 aggtagacct gtaacaggac ctggaaatag accacttaaa tctttatcag atatgttaaa
     9961 aggtaagcaa ggtcgtttcc gtcaaaactt acttggtaag cgtgttgact actcaggacg
    10021 ttctgttata gttgttggac cagaacttaa attttatcaa tgtggacttc caaagaagat
    10081 ggcattagaa ttattcaagc catttgttat ggataaatta gttaaagaag gttatgcaca
    10141 taatataaaa agtgcgaaat ctatagtaga aaaagttaag ccagaagttt gggatgtttt
    10201 agaagatgtt ataaaaagtc atccagttct tcttaaccgt gcaccgactc tacatagatt
    10261 aggtatacaa gcatttgaac caatcttagt tgaaggtaag gctataaaac tacatcctct
    10321 tgtatgtaca gcttataatg cagactttga tggtgaccaa atggcagttc atgtaccttt
    10381 atcagtagaa gcacaagcag aagcaagatt cttaatgctt tctgtaaata atatacttgc
    10441 tcctaaagat ggttcaccta taactactcc atctcaggat atggttttag gttgctatta
    10501 tctaacaata gaagcccaag atggagctaa aggaacaggt atggtattta aagactttaa
    10561 tgaactatta cttgcttatt acaataaatc agttcatcta catgcattag tgaaacttaa
    10621 ggtaacactt gaagatggaa gaagttcatt ggttgaaagt actgttggta gatttatatt
    10681 taatgaaaat atacctcaag acttaggttt tgtagataga aaagaaaatc catttgcact
    10741 tgaagttgat ttcttagcag ataaaaaatc tcttggtaaa ataatagata aatgctttag
    10801 aaaacacgga aatacagaaa cagctgaatt actagattat ataaaagctc taggatttaa
    10861 atattctaca ttaggtggca taacagttgc tgttgatgat atgagtgtcc cagaagaaaa
    10921 gaaagtattt atagctgagg cagaagctaa agttgataag tatgaaaaag catacagaag
    10981 aggtctaatc tctgatgaag aaagatatga aaaagttata gagacatgga cagaaacaac
    11041 tgataaagtt actgatgctc ttatgggtgg actagataga ttaaataata tatatataat
    11101 ggcacattca ggagccagag gttctaaaaa ccaaattaga cagctggcag gtatgcgtgg
    11161 tcttatggca aatgcatctg gtaaaacagt tgagatacca gttaaatcta atttccgtga
    11221 aggtttatca gtactagaat actttacatc ttcacatggt gccagaaaag gtcttgccga
    11281 tacagctata cgtacagctg aatctggata tttaacaaga agacttgttg atgtaagtca
    11341 agatgttatt gtaagggaaa tagattgtgg aacagaagat actacagaga tttatgctat
    11401 aaaagaagga aatgaagtta tagaagagat atatgataga attgtaggaa gatatactat
    11461 agaccctata ttaaatcctg aaactggaga agttatagtt gaggctgatt caatgataca
    11521 agaagatgaa gcagaaacta tagtagcttt aggaattgaa aaaattagga taagaacagt
    11581 tcttaactgt aaaactaatc atggagtttg ttctaagtgt tatggtagaa acttggcaac
    11641 aggaaaagaa gttaatatag gtgaagcagt tggtataata gcagctcaat ctatcggtga
    11701 gccgggtact cagcttacaa tgcgtacatt ccatactgga ggagttgcag gagctgatat
    11761 aactcaaggt ctaccaaggg ttgaagaatt atttgaagct agaaaaccaa aaggattagc
    11821 tgtaataact gaagtatctg gtagagttga aattgatgaa acaggaaaga gaaaagaagt
    11881 aaatgtaata ccagaagaag gcgagactca aacatatgta attccatatg gttctagatt
    11941 aaaagttaag caaggtcaaa tgctagaggc tggagaccct ctaacacaag gatttataaa
    12001 tcctcatgat atagtaagag taaatggtgt taagggagtt caagaatata tagttaaaga
    12061 agttcaaaga gtgtatagac ttcaaggggt tgatgttaat gataagcata tagaagttat
    12121 agtaagacaa atgctatcta aagtaaaagt tgaagaccca ggagatacag atttattgcc
    12181 aggtggatat gaagatgtat taacattcaa tgaatgtaat aaagatgcta tagataaagg
    12241 tctaagacca gcagttgcta aaagagtttt acttggtata actaaagcat ctcttgcaac
    12301 tgattcattc ttatcagcag cttctttcca agaaacaaca agagtattaa cagaggcagc
    12361 aataaaaggt aaagaagacc acttaatagg acttaaagaa aatgttatat taggtaagtt
    12421 aataccagca ggaacaggaa tgaagaaata tagaaatata gctgttgaaa aaattgaaga
    12481 ttaatagcat atagagtaaa gaaatcttga caaccattat ttgtggtgtt aaaatagtaa
    12541 agtgtgttgt ttaaaagtac actaagggct tgaacaagtt cttagtgact tttagatggc
    12601 aataaatgag aactaaaaag catcaagcta gaaaagtgct aatatcacat atttatgtgt
    12661 tatagacatt aaattgtaag taacgaatgt tacaatcagt cagtatgaat attcatattg
    12721 atgtagttgg cggtcactga taagatcgaa aatttggaag gaggtgcaga tgatgccaac
    12781 aattaaccaa ttagttcgta aaagtagaaa agcgctagag aagaaatcta ctgctccagc
    12841 attacaaaaa ggttacaact ctttaaataa gaaagtaact gacgctagtg ccccacaaaa
    12901 aagaggggtt tgtacttcag taaaaacagt tactcctaag aagcctaact cagctttaag
    12961 aaaagttgcc agagttagat taactaatgg tatagaagtt tctgcttata taccaggaga
    13021 aggacacaac ttacaagagc atagtgttgt tcttataaga ggaggaagag taaaagacct
    13081 tccaggggtt agataccata tactaagagg tactctagat acagcaggtg ttgataagag
    13141 aagacaatca agatctaaat acggtgcgaa gagacctaaa gaagctaaaa agtaaaaaca
    13201 attaatgatt gattatagtt cggtgcttta aatatatata tatttacggc atcacggcat
    13261 taaatgattt atgtcgagta cctatgattt aagttaaata attaaggagg gaagcgaaat
    13321 gccaagaaaa ggtaatattc caaaaagaga agttttacca gatcctatgt atgggagtaa
    13381 ggtagttact aaattaataa ataatctaat ggtagacgga aaaaaaggaa agtctcaaag
    13441 aatagtttat gatgctttcg caattgttgc tgaaaagaca ggagaagaag ctttagaagt
    13501 atttaataaa gcaatggata atataatgcc tgttttagaa gtaaaagcaa gaagagttgg
    13561 tggggcaaac taccaagtgc caatcgaagt tagacctgaa agaagacaaa cattaggttt
    13621 aagatggcta gtaaaatata ctagagctcg tggagaaaaa ggaatggttg aaaagctagc
    13681 taaagagata atggacgcag ctaacaacac tggagcttca gttaagaaaa aagaagatac
    13741 tcataaaatg gcagaagcta ataaagcatt tgctcattac agatggtagg atatatagat
    13801 atcttataaa attgaggata tcataaggag gataaccatg gctagaaagt ttcctttgga
    13861 aagaactagg aatataggaa tcatggctca tatagatgca ggaaaaacta ctactacaga
    13921 aagaatcctg ttttatactg ggcaaactca taaaatagga gaaactcatg aaggagcttc
    13981 tcaaatggac tggatggagc aagaaaagga aagaggtata acaataactt ctgccgctac
    14041 tactgcttca tggaaagacc atagaataaa cataatagat actcctggac acgtggactt
    14101 cacagttgag gttgaaagat ctctaagagt tcttgacggt tcagttgctg tattctgtgc
    14161 aaaaggtggg gttgagcctc aatcagaaaa tgtatggaga caagctgaaa cttatggagt
    14221 acctagaata gcttttgtaa ataaaatgga tatattaggt gcagatttct acaatgttgt
    14281 aagcatgatg aaatctagat taaattctaa tgcagttcct atgcaattac caataggtaa
    14341 agaagattca tttataggta taatagactt attaaaaatg gatgctgtta tatacaaaga
    14401 cgacctaggg gttgaaatgg aagaaacaga tataccagaa gatatgaaag aattagctgc
    14461 tgagtggaga gaaaaattag ttgaatcagt agctgaaact gatgaagaat taatgatgaa
    14521 ataccttgaa ggtgaagaac ttactataga tgaattaaaa gttgctataa gaaaagctac
    14581 tatagcttgt gaaatgaacc cagttttctg tggtacagct tatagaaaca aaggtgttca
    14641 gttagtaata gatgcagttt tagattactt accagcgcca actgatatac cagctataaa
    14701 aggtatatta gcagatggag aagaagctga aagacattct tctgatgaag agccattctc
    14761 tgcattagca ttcaaaataa tgactgaccc attcgttgga aaactagcgt tcttcagagt
    14821 ttattctgga acattagaat caggttctta tgttttaaat gcaactaaga ataagagaga
    14881 aagaattggt cgtatattac aaatgcatgc taatacaaga gaagaaataa ctaaagtgta
    14941 tgctggagat atagcagcag cagtaggatt aaaagatact actactggag acacattatg
    15001 tgaccctgct aatccaataa tacttgaatc tatggaattc cctgagccag ttatatctgt
    15061 tgctatagaa ccaagttcta aagcagctca agaaaaaatg ggtatagctc ttcaaaaatt
    15121 agctgaagaa gacccaactt tcacagttaa gactgaccaa gaaacaggac aaactataat
    15181 atctggtatg ggtgaattac acttagagat aatagtagat agattattaa gagaatttaa
    15241 agtagaagct aaagttggtg caccacaagt tgcatataga gagactataa ctcaaccagt
    15301 tgatgttgaa tacaagtact ctaagcaatc aggtggtaga ggacaatacg gtcacgttaa
    15361 gattagagtt gctcctcaag agccaggtga aggatacaaa ttcacaaaca aaactgttgg
    15421 tggatcagta ccaaaagaat atgttggtcc agttgacatg ggtatacaag gagctatgca
    15481 atctggtata gttgctggat atccagttgt agacgttgct gttgagttat atgatggttc
    15541 ttaccatgag gttgactctt ctgagatggc gtttaagatg gctggttcaa tggcattcaa
    15601 agatgctatg aagaaaggta atgcagttct tttagagcct tacttcaaag tagaagttgt
    15661 tactccagaa gattacatgg gagatgttat gggtgactta aactcaagaa gaggattaat
    15721 ccaaggtatg gaagctagaa gtggtgcaca agttataaat gcattcgttc cactttcaga
    15781 aatgtttgga tactcaacag acctaagatc ttcaactcaa ggtcgtgcaa cttacacaat
    15841 gatattcgat cactatgagc aagttccagc ttcagttgct aagaaaatag ctgaaggaaa
    15901 ataatttaaa aataattaat aatataaata taaaaaataa tcagctagat gagagatatc
    15961 tctcatctag cacaagat
//
LOCUS       DAMJYR010000086        15954 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.088, whole genome shotgun sequence.
ACCESSION   DAMJYR010000086 DAMJYR010000000
VERSION     DAMJYR010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15954)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15954)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15954
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.088"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            282..2294
                     /locus_tag="PC471_002623"
     CDS             282..2294
                     /locus_tag="PC471_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDF2789741.1"
                     /translation="MKKSVALVNDSRKDLIDFLENNLKLVFGDSININRYFINEINDN
                     DIINDDVILVMSVERLDKIINNILDKKKVIVVRRTFREDKIYNLLSLPQGTNVLIVND
                     SDETTLETISLFYKIGVTNIRPIPYMNDNNYKNIKIAITPGVPEKVPSFISDIFDLGH
                     RYIDISTFIEIINLLQIDSKEIQSNLVKYSEEIISLDTGIKDKYKELFLKIEELDTIL
                     NLSKDGILFTSKDGEINTYNSKVKDILDINEDIYGKYIEDIFVDSLKVLLSEKEILDK
                     VVVFNKKYINVNKKNIYNRDEKMGTYYSLQEITYIKKLEQNLTKKLREKGQIAKYTFK
                     DIKTNSPKMFECIDLAKKVSKSDLSILIRGESGTGKELIAQSIHNNSNRKNQPFIAVN
                     CAAVPENLLESQLFGYDKGTFTGGLKDGKQGLFELANNGTIFLDEIGDMPLELQTKLL
                     RVLQEKQIMPVGSHNVINIDVRIISATNKNLEQMIDNSQFREDLYYRLNTIPINIPPL
                     RERKEDILIIMEDLINKKLVITPEAKKLIQNYMWKGNIRELQNVTSYLNIMCEDIVLE
                     KDLPPNLRSSDNKNTSLKLKYSKNDILNILEILILNKESDVGIGRGLILKALLDKNLQ
                     ITEGKIKKIFEYLKKEELIICSSGRYGSKITQKGEDFYNKLKYKGL"
     gene            2663..3883
                     /locus_tag="PC471_002624"
     CDS             2663..3883
                     /locus_tag="PC471_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HDF2789742.1"
                     /translation="MQQALKLKYQTKELGKDFVIESCNSIKPWLINIRRELHKIPELA
                     LEENLTKQKVISYLKEIGIDYMEFTKHNGIMAYILKESADKTICIRADMDALPIEEEN
                     NIPYKSIHSGKMHACGHDAHTTMLLGACKVLHSIKDKLNVNVKFLFQPAEEGFGGAKF
                     LVEDGCLENPKVDYIFGLHVMPHIETGFIEIKYDTLNASVDTIKISIKGKRAHGAYPE
                     NGIDAIVTASQIVTSLQTIISRNLEPNNAAVLTIGKIYGGDAHNVICEDVKLEGTLRT
                     LNSKTRNFMIDKIAKIVGDTASAFGCVGTLHVSDENYPAVINEKELVDTVISNTKELL
                     GEEKFILRPNPSLGGEDFSFYTEHCKGAFFHLGCKNEEKGLISPLHTSSFNIDEDCLP
                     IGVMMHVMNTLYFN"
     gene            3920..5269
                     /locus_tag="PC471_002625"
     CDS             3920..5269
                     /locus_tag="PC471_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDF2789743.1"
                     /translation="MEKAKKLGLFSMILLGINSIIGSGIFLLPGKVYNLAGQNSMFIY
                     IFATLLVLSILLCFAEVGSMFDKNGGAYLYSKKAFGDFIGFEVGTMSWVIRIISWSTL
                     AVGFATALGSFWPESATEYKGYIAAILVTLLSINSLFGIKSTKIMNNVITIAKLVPLI
                     VFIIVGIFFIKFVNIVPSGNVVNSSMGPAIILVFYAFTGFESFIVASGEMENPKKNLP
                     VALITTIFICAIIYILIQIVCMGILGDRLFENSIPIADASSVFLGNYGKFFISVATLI
                     SIFGINIGSSIVTPKCGSSLAEEGSLPAFIGKTNKYGAPYVAIIISLICCIPLVLTGS
                     FEQLAVMSVIARFAQYIPTCLSVIVLRKRTDVKASFKIPFGPVIPMVAILGSLWLLQQ
                     AWAEDISKPITQNRVLIGLGAMLLIAPLYIFMKKNKEIEHKNKSEDSKLYISREKIQ"
     gene            5383..5945
                     /locus_tag="PC471_002626"
                     /pseudo
     CDS             5383..5945
                     /locus_tag="PC471_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901678.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(6053..8386)
                     /locus_tag="PC471_002627"
     CDS             complement(6053..8386)
                     /locus_tag="PC471_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HDF2789744.1"
                     /translation="MRSYLELVPQYAKVHKKKNRTTILCIVIAVCLVTAIFGLADMAI
                     QCQKVQFIKDDGNWHIVLKNLNEEAISEISSRVDIAHYGRGQVIDNGEFKSKEILIQG
                     LDEDLAKQLGLEIIKGRYPVVENETLIDVDAVKSFNLKVGDFIKIKLADGRNKNYKIV
                     GEYSSLSRLRASDKHGLMLSILGTQAIKKSETYDYYYITFKDKVNIRKATSDIKNTFN
                     LSDKQIGKNEKLLSIIGQGDSDYSKPLYLTAGGLFILVLIASIIMIYNSFNMGVIERI
                     KFFGLLRCLGASKLQVKKFVVLESLILSVKAIPLGLLLGCIVTIISSIFLKYVNTELF
                     GSMPILKVSFIGIVFGIIVGFLTVILSAIVPAKKASGVSPLSAIRGNLMFKEILTSGK
                     ASKIANAGSSKIDVSMGINHAFSRKKSFILMTSSFAISIVLFLGFSVFIDFMYQALKP
                     IKPEAAHISIVDADFKSVLTKDDINEISNIKGIKKVYSRMMTDVEATYNNKEATSQLI
                     SYEKNQFDWAKKYLVKGEINEDKLESENIGLVEEGHGFNIGDKIYIKNGNSKKEIEVA
                     GILSSISFDVKDKYIGNIITSENTFTSITDIKDYIIIDTQVDEDAPEDLIQNIRNVLD
                     DNLEVRDKRQGNLEAKNSFYTMAVFVYGFVFIIAIISMFNIINSMNISVTSRINYYGI
                     MRAIGMSNKQLRKMVIVESSTYAVSGCLLGSVLGLILHRYIFVSLVTLKFHIDWQIPF
                     DLLFIIVVTMIIITLLAVRKPIKKICELDIIEVVNAQ"
     gene            complement(8383..9066)
                     /locus_tag="PC471_002628"
     CDS             complement(8383..9066)
                     /locus_tag="PC471_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2789745.1"
                     /translation="MDILVVNNLCKTYGNGYTKVEALKDVSFTVSKGEFVTVVGESGS
                     GKSTLLNLLGGLDTPTSGKIFVDGKEICSMKEEKQTIFRRRNIGFIFQSYNLIPELTV
                     EQNIIFPLLLDYKKPDTIYLDEVLTILGLKDRKSHLPNQLSGGQQQRVAIGRALITKP
                     SIILADEPTGNLDSKNSSDVISLLKVTAEKYNQTIIMITHNTNIASTADRILQVSDGM
                     LKDLGVNRE"
     gene            complement(9137..10378)
                     /locus_tag="PC471_002629"
     CDS             complement(9137..10378)
                     /locus_tag="PC471_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF2789746.1"
                     /translation="MKVFSNKDIKIFFLVIISVLLMFIVVGQIVVINITNDYKSALLE
                     HDYNIAGYLNSMGVDKSKIPAVFTTNEDKYTLKGKEILNTARYDFGTDDSFMPSVKLF
                     QGKYIKTMFLYLFVFFTMIIFILYVYFFRQQNKIEEASYKIDSFMNGNINVRLNTYEE
                     GSLSKLFGSIDAMSTSLNTHILKEKQNKEFLKNTISDISHQLKTPLTALMMYNQILQE
                     ESHNSEIVENFVQKSQNELERIESLIQNLLKITKIDSKTIILNKASVNVKKLITKILY
                     SFETRAEKEGKSISIRGLENTNLFCDYEWISEALSNLIKNALDNTKENDKIMIEWVET
                     PITTIISVSDTGNGIHIEDIHHIFKRFYRSKFSKSNQGLGLGLPLVKSIIEQHNGTIT
                     VESNFLQGSTFTLSFLKLTNM"
     gene            complement(10389..11075)
                     /locus_tag="PC471_002630"
     CDS             complement(10389..11075)
                     /locus_tag="PC471_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2789747.1"
                     /translation="MSSILLVEDDLSLIYGLEYSIQKNGFSVDVARTVEEALQIYKEK
                     NYDLLLLDVSLPDGDGFDICKRVREASNVPIIFLTASDEEVNVVMGLDMGGDDYITKP
                     FKLNELISRIKALLRRYNFTSENVTELKSNNITVKLLENRVFKNEIELELTTAEYKLL
                     CLLMKNKNIVLTRKNILDKLWDGNGSFVDDNTLSVYVRRLRNKIEDNPENPKNLLTVR
                     RMGYKWNVIK"
     gene            complement(11313..11657)
                     /locus_tag="PC471_002631"
     CDS             complement(11313..11657)
                     /locus_tag="PC471_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2789748.1"
                     /translation="MYCLSVVLAIYGIYVFINCTEYIYTMLSTKHLTVKGNEYTIINY
                     YMSNFSQYIIFAILLASMGFVIQKIVHMHFSEIELSGNYSTTVRNISDISLDNDNEMF
                     NRGNIENSILKK"
     gene            complement(11988..14495)
                     /locus_tag="PC471_002632"
     CDS             complement(11988..14495)
                     /locus_tag="PC471_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2789749.1"
                     /translation="MKLNYIVKKYIMGNKKNTLLIIVSIVISTALFLIMNIISEDATN
                     LMINQAKNEFTLKHAQYINPKDEEIKYIENNTSIDKVGKSMLLGISDIGKGQTLQILS
                     QDKVAEELNDIYTLEKGKLPVAENEIAIDSWYIKQKKIKNPIGKRITLDYRRQGIDQE
                     DLYTGEKEFKITGILNSNPILKAQGTSIGLISNDCAIKNIPTKNKYDQVMFTFKKEKN
                     IQRQSQKLIKNGNLNENNIYFNNELLIAISDSMNLKIPYIIVNIVLALATILLIYNIF
                     YILVSNRIKDFGIFRALGFNPNDIFKIMILEVSIYSIISISIGLILGGIIASLSREYV
                     IGVIYNVNYINSIKNQNYINTYIISILLSLGTIIISVSKPLMLSVKTDPMICIRRNNE
                     KVDIKQNSFINKFMIKLFKDYGNIASKNIQRNKKRTNLSIASMVIIFFLMATIYTKST
                     SNFLSDGVLKYWIQGDYLLHNIDISTIRSNNKGYDKNVLQEIKAIDGVTKVNSYRHKW
                     FNIKIDDKSINKNSDYWKKNKDNIEARSEVKDGIKVYNNSFEFLGIEDTDILEDFLIN
                     GKENIDKFSKQPYLYITKKSSDSLNIKKGDRIKVYFDIIDSKTNNFTKTISQEFIVCG
                     IITTLPVTSQVGAEFGGVISTNQFNKFTGISSYERFDIWTSKLANDKYVESELNKITE
                     KTNKGILIPYKAETAEYEKIDNQKTLIMVLVTGIIVILSLFNCCNTIVTSINSRSREF
                     ALFRGIGISKHEINKIVKLESYIYIIVSFCISIIPILIVRSIIIKPFESINLINWKFI
                     GAIILIVFILVSIIMITTLKTLNKIQNENFMEEIKTL"
     gene            complement(14504..15166)
                     /locus_tag="PC471_002633"
     CDS             complement(14504..15166)
                     /locus_tag="PC471_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2789750.1"
                     /translation="MSILEIKNLNKIYGKSETEVKALSQINLKINSGEFVVIVGKSGS
                     GKSTLLHIMAGLETPTNGDVIIDNINISSLDEKKRSALRKEKLGLIFQSYNLIPVLTV
                     EENIKLPTINIKNKDEAYINELMELLGIKDRRMYLPNQLSGGQQQRVAIARALVNKPS
                     IVFADEPTGNLDTKTESDVLSLLKESQKKYNQTIIMITHNIDITKYADRVIEIEDGFI
                     RE"
BASE COUNT         5677 a         2270 c         1877 g         6130 t
ORIGIN      
        1 attatattat caatagaatt tattaaaata aatttttttg ggtgtatatt ataatttaat
       61 cgaatagaaa tgaaaaatat acatacagtt taagttttaa gcaacaatta gggggatttg
      121 gtgacaagat ttttaattat aagaaagaaa gtgtcttaat gcaactttta taaagtcatt
      181 atagacactt tcttttttat ttgataaatt taaattatca aaattatatt gctttataca
      241 actattttgt tggaaactat ttattatata aggagaaata aatgaaaaaa tcagtagctc
      301 tggtaaatga tagtagaaaa gatttaatag actttttaga aaataatttg aaattagttt
      361 ttggagattc tataaatatt aatagatact tcataaatga aataaatgat aatgatatta
      421 taaatgatga tgtgatatta gtaatgtcag tagaaagatt agacaaaatt ataaataata
      481 tactagataa gaaaaaagtg attgtggtaa gaagaacttt tagggaagat aaaatttata
      541 atctactatc tttaccacag ggtacaaatg ttctaatcgt aaatgattct gatgaaacta
      601 ctctagaaac tataagttta ttttataaaa ttggagtgac taatattaga ccaattccat
      661 atatgaatga taataactat aaaaatataa aaatagctat aactccagga gttccagaaa
      721 aagtacctag ctttatctca gatatatttg atttaggtca cagatatata gatatttcta
      781 catttataga aataataaat ttattacaaa ttgattcaaa ggaaatacaa tctaatctag
      841 ttaaatactc agaagaaata ataagtttag atactggtat aaaagataaa tataaggaat
      901 tatttttaaa aatagaagaa ttagatacaa tattgaattt atcaaaagat ggtatattat
      961 ttacttcaaa agatggtgaa ataaatacat ataatagtaa agtaaaagat attttagaca
     1021 taaatgagga tatatatgga aaatacattg aggatatatt tgtagatagt ttaaaagttt
     1081 tattgagtga gaaagagata ctagataaag ttgtcgtatt caataagaaa tatataaatg
     1141 taaataaaaa aaacatatac aatagagatg aaaaaatggg cacatattat agtttacaag
     1201 aaataacata cataaaaaaa ttagaacaaa atctcactaa aaaattaaga gaaaaaggac
     1261 aaatagcaaa atatacattt aaggatataa aaacaaatag tcctaaaatg tttgaatgta
     1321 tagatttagc taagaaggtt tctaaatctg acttaagcat acttatcaga ggtgaaagtg
     1381 gaactggtaa agaactcata gctcagtcta tacataacaa ctcaaataga aaaaatcaac
     1441 cctttatagc agtaaattgt gcagccgttc ctgaaaattt attagaaagc cagctattcg
     1501 gatatgacaa gggaactttt actggaggtc taaaagatgg aaaacaagga ttatttgaac
     1561 ttgctaataa tggaacaata ttcttagatg aaataggcga tatgccttta gaattacaaa
     1621 caaaactact tagagttctt caagaaaagc agataatgcc tgttgggtct cacaatgtaa
     1681 ttaatataga tgttagaatc atatctgcta caaataagaa tttagaacaa atgattgata
     1741 atagccaatt tagagaggat ttatattatc gattaaatac aattccaata aatatacctc
     1801 ctctaagaga aagaaaagaa gacatattaa ttattatgga agatttaatt aataagaaat
     1861 tagtaatcac accagaagca aaaaaactta tacaaaacta tatgtggaaa ggtaatataa
     1921 gagaactcca aaatgttaca tcctatttaa acattatgtg tgaagatatt gtattagaga
     1981 aagatttacc tccaaactta aggtcttcag ataacaaaaa tacatcatta aaactaaaat
     2041 atagtaagaa tgatatattg aatatattag agatactaat tttaaataag gaatcagatg
     2101 ttggtattgg tagaggatta attttaaaag cactactcga taagaacctt caaattacag
     2161 aaggaaaaat taaaaaaata tttgaatatc taaaaaaaga agaacttata atttgtagtt
     2221 caggaagata tggaagtaaa ataactcaaa aaggagaaga tttttacaat aaattaaaat
     2281 ataaaggtct ataaaattat aatttatcaa acaaagaaat ctattcagga ttaaagtact
     2341 ccttcaatag gtttttttat ttttcaagta ccctgaaatt ataatttatt ttgtacataa
     2401 ataataggaa ttaataggaa taggataatt tttttatttc ctattttttt tatataaact
     2461 agcatataaa atatattttc ttcaaaataa tattttttac tgaaaattga aactatgcaa
     2521 tcatttttta tactatacat atagataaat gattatattt attcaatatt cttataaaaa
     2581 acaaagttct ccatcgaaaa ttatggcata ataattgctt attatattaa taaaacaata
     2641 aaaagcaatt ggaggaataa aaatgcaaca ggctttgaaa ttaaaatatc aaaccaaaga
     2701 attaggaaaa gattttgtaa tagaaagttg taattctata aaaccatggc ttataaacat
     2761 aagaagagaa ttacataaaa ttccagaatt agctcttgaa gaaaatctta ctaaacagaa
     2821 ggtaatttct tatttaaaag aaatcggaat tgattatatg gaatttacaa aacacaatgg
     2881 aattatggca tatattttaa aagaaagtgc agataaaacg atttgtataa gagcagatat
     2941 ggatgctctt cctattgaag aagaaaacaa cataccttat aaatcaatac attctggaaa
     3001 aatgcatgct tgtggccatg atgcacatac aaccatgcta cttggagcat gtaaagtttt
     3061 acattcaata aaagataaac ttaatgtaaa tgtaaaattc ttatttcaac cagcagaaga
     3121 aggttttgga ggagcaaaat tcttagttga agatggatgt ttagaaaatc ctaaggtcga
     3181 ttatatattc ggacttcatg ttatgccaca tatcgaaaca ggatttatcg aaataaaata
     3241 tgatacatta aatgctagtg tagacactat aaaaataagt atcaaaggta aaagagctca
     3301 tggtgcatat cctgaaaatg gtattgatgc aatagttaca gcttcacaaa ttgttacatc
     3361 attacaaact ataataagta gaaacttaga accaaataat gctgctgttt tgactatagg
     3421 taaaatatat ggtggtgatg cacacaatgt aatatgtgaa gatgtaaaac tagaaggcac
     3481 tttaagaacc ttaaatagca aaacaagaaa ctttatgatt gataaaatag ctaaaattgt
     3541 tggagacact gcatctgcat ttggttgtgt tgggacttta catgtaagtg atgaaaacta
     3601 tcctgctgtt ataaatgaaa aagaattagt agatacagtt atatcaaata ctaaagaatt
     3661 acttggagag gaaaaattta tattgagacc aaacccgtct ttaggtggtg aagacttttc
     3721 tttttacaca gaacattgta aaggagcatt cttccattta ggatgtaaaa atgaggaaaa
     3781 gggcttaatt tcacctcttc atacctctag ctttaatata gatgaagatt gtttaccaat
     3841 aggtgttatg atgcatgtaa tgaatacttt gtattttaat tagtacagta attaataaaa
     3901 gaataggaga taaattaata tggaaaaagc taagaaatta ggtctattta gtatgatatt
     3961 attagggatt aatagcataa taggttcagg tatattctta ctaccaggta aagtttataa
     4021 tctagcaggt caaaacagta tgtttatata catttttgct acactcttag ttctatcaat
     4081 acttttatgt tttgcagaag ttggaagcat gtttgataag aatggaggag catatctata
     4141 ctccaaaaaa gcttttggag attttatagg atttgaagtt ggtacaatgt cttgggttat
     4201 tagaattata tcatggtcta ctctagcagt aggatttgca acagctttag gttcattttg
     4261 gccagaatct gccactgaat acaaaggtta tatagctgca atactcgtaa cactactttc
     4321 aataaatagc ttatttggaa ttaaaagcac aaagataatg aataatgtaa taactatagc
     4381 taaattagta ccattaatag tttttataat agttggaata ttctttataa aatttgtaaa
     4441 tattgttcct tcaggaaatg tagttaactc tagtatgggt ccagccatta tattagtatt
     4501 ttacgcattt accggctttg aatcatttat tgtggcaagt ggagaaatgg aaaatcctaa
     4561 aaaaaatctt ccagtcgcat taataaccac aatatttata tgtgcaataa tttatatatt
     4621 aattcaaatt gtctgtatgg gtatacttgg agatagacta tttgaaaaca gtattccaat
     4681 tgcagatgct tcaagtgtat ttttaggcaa ttatggtaaa tttttcatat ctgtagcaac
     4741 attaatatct atatttggta taaatatagg ttcatcaata gtaactccta aatgtggttc
     4801 ttctctagca gaagaaggtt ctcttcctgc atttatagga aaaacaaata aatatggtgc
     4861 tccatatgta gcaataataa tatctttaat ttgttgtata ccacttgtgc taactggaag
     4921 ttttgaacaa cttgcagtaa tgagtgtaat tgcaagattt gctcagtaca taccaacttg
     4981 tttatcagta atagttttaa gaaaaagaac agatgttaag gcctctttta agattccatt
     5041 tggtcctgtt ataccaatgg tagctatttt aggtagctta tggttgttac aacaagcttg
     5101 ggcagaagat atttcaaaac ctataactca aaatagagtt ttaataggat taggggcaat
     5161 gttgttaata gctcctctat atatatttat gaaaaaaaat aaggaaatcg agcataaaaa
     5221 taaatcagaa gattctaaat tatatatttc gagagaaaaa attcaataat attttacaaa
     5281 ctatttaaaa attatttttc taatactata gaaataatta gaaatggaga tgttacgtat
     5341 ctaaaagtag ttgataacac ggatattggt acttttatag atatgggact tacaaaagac
     5401 atactagttc cttttaaagc taagacttat ccattgttta gagatgaaaa atatttattc
     5461 tatatatact tggataaaag cggaagaata gctgctacta cagacataga ttcatattta
     5521 gaaacagacc atacttacaa tgtaggagat gttgtaaatg gagttgtata tggattccaa
     5581 accaacaact ctgctatgat atgtgttgac aataaatatg ctggtgtaat ccttcataat
     5641 gaatacttta cagaattaaa agctggggat gttcttgaaa atttacatgt tataaaaata
     5701 tacgatgatg gtaaattagg actttctcct agaggaaaca gaaaagatga acttgatacc
     5761 ttagaaaata agattttaag ctatttagaa ggttcagatg gatatatgag attcaatgat
     5821 aaatctgatc ctaaagatat ttctatctta tttaattcaa gtaagaaaat tttaaacgtg
     5881 ctcttggagt acttatgaag aaagacttaa tttatcaaga tgaagatgga acttatctaa
     5941 agtaaattat ataacacaaa aatcgaagta aattatgaaa gatattttct atttcatatt
     6001 tacttcgatt ttttatttaa attactacct ctatttaaat tactacttat atttattgtg
     6061 catttactac ctctattatg tctaattcac aaatcttttt aattggtttt cttactgcaa
     6121 gcaaagttat tattatcata gttactacaa tgataaacag taaatcaaaa ggtatttgcc
     6181 agtctatatg gaattttaat gttactaagg atacaaatat atatctatgt aatattaatc
     6241 caagtacact ccccaataaa cagccactta cagcatatgt acttgattct actattacca
     6301 tttttcttaa ttgcttattg gacatgccta ttgcacgcat aataccatag taatttattc
     6361 tacttgtaac gcttatattc atactgttta tgatattaaa catacttata atcgcaatta
     6421 taaatacaaa cccatataca aaaactgcca tagtgtaaaa tgaattttta gcctctaagt
     6481 taccttgtcg cttatctcga acctctaaat tatcatctag aacatttctt atattttgga
     6541 ttaaatcttc aggagcatct tcatcaactt gagtatcaat tattatatag tcttttatat
     6601 ctgttatact agtaaaagta ttttcagatg ttataatatt tccaatatat ttgtctttaa
     6661 catcaaatga aattgaagaa agtatacctg ctacctctat ctctttctta gaatttccat
     6721 ttttaatata tatcttatct ccaatattaa acccatgacc ttcttctacc aatccgatat
     6781 tttctgactc taatttatct tcattgattt ccccttttac tagatacttt ttggcccagt
     6841 caaattgatt tttttcataa gaaattagct gagatgttgc ctccttatta ttgtatgtag
     6901 cctctacatc cgtcatcatt ctactgtata ctttttttat tcctttgata ttacttattt
     6961 cattaatatc gtctttagta agaacagact taaagtcagc gtcaacaata gatatatgag
     7021 cagcttctgg ttttataggt ttaagtgcct gatacataaa gtctataaat acactaaaac
     7081 ctaaaaacaa tacaatacta atagcaaatg aagaagtcat aagtataaaa gatttttttc
     7141 ttgaaaaggc atgatttatt cccatagaaa catcaatttt agaacttcct gcgttagcaa
     7201 ttttacttgc ttttccacta gttaaaattt ctttgaacat tagattacca cgtatagcac
     7261 ttagtggaga caccccagaa gctttttttg ctggaacaat agctgataat attactgtta
     7321 aaaatcctac tattattcca aacacaatac caataaagct aacctttaaa attggcatcg
     7381 aaccaaataa ctctgtattc acatatttga gaaatataga ggatataatg gttacaatac
     7441 atcctaataa caatcctagt ggaatagcct ttacacttag aattaaactt tctaaaacaa
     7501 caaatttttt gacttggagt tttgatgctc ccaagcaacg taatagtcca aaaaacttta
     7561 ttctctctat tacacccata ttaaagctat tatatatcat gataatactt gctattaaca
     7621 caagtataaa aagtcctcct gctgttaagt aaagtggttt agaataatca gagtcacctt
     7681 gacctataat agaaagtaat ttttcatttt taccgatttg tttatcactt aaattgaagg
     7741 tattcttaat atcagaagta gcttttctaa tattaacctt gtctttaaat gtaatataat
     7801 aataatcata tgtttctgat tttttgatag cttgtgtacc taatattgaa agcattaatc
     7861 catgcttatc tgaggctctt aatcttgata atgatgaata ttcacctact atcttataat
     7921 ttttatttct accatctgct aattttattt tgataaaatc tcctactttt agattaaagc
     7981 ttttaactgc atctacatct attaaagtct cattctccac aacaggataa cgacctttta
     8041 ttatttctag acctaattgt tttgctaaat cttcatctaa accttgtatc aaaatctcct
     8101 ttgatttaaa ctctccatta tcaatgactt gtcctctacc atagtgagct atatctactc
     8161 tactacttat ttctgatata gcttcttcat taagattttt aagtactatg tgccaatttc
     8221 catcatcttt tataaattgt actttttgac attgtatagc catgtctgct agtccaaaaa
     8281 tagcagtaac taaacatact gcaatgacaa tacataaaat tgtagttcta ttttttttct
     8341 tatgtacctt tgcatattgt ggtactagtt ctaaatagct tctcattctc tattcacccc
     8401 taggtcttta agcattccat cagaaacctg tagtatccta tctgctgtag aagctatatt
     8461 tgtattatga gttatcataa taatagtttg gttatatttt tctgctgtca cttttaaaag
     8521 tgaaattaca tcactgctat ttttactgtc aagatttcct gttggctcat ctgccaatat
     8581 aattgatggc tttgtaatca atgctcttcc aattgcaacc ctttgttgct gaccaccaga
     8641 taattgattt ggaagatgac tttttctatc tttaagacct aaaattgtca atacttcatc
     8701 tagataaata gtgtctggtt ttttgtaatc tagtagtaaa ggaaatatta tattttgttc
     8761 cacagttaat tcgggtataa gattatatga ttgaaagata aaacctatat tacgacgtcg
     8821 aaaaatagtc tgtttttctt ctttcattga acatatttct ttaccatcaa caaatatttt
     8881 tcctgaggtt ggagtatcaa gacctcctaa aagatttaat aatgtactct tacctgagcc
     8941 agattctcca actacagtaa caaactctcc ctttgaaact gtaaatgata catcttttaa
     9001 tgcctctact tttgtatatc catttccata tgttttacat aaattattaa ccacaagaat
     9061 atccatttgc tctatctcct ttctgatgat taatataaga ataattcata caccttactg
     9121 taagatgtat cctatcttac atattagtaa gctttaaaaa acttagagta aatgtacttc
     9181 cttgtaaaaa attactttct acagttatgg ttccattgtg ctgttctatg atagatttaa
     9241 ctagtggcaa ccctaatcca aggccttgat tactttttga aaacttactc ctataaaatc
     9301 ttttaaatat atgatgtata tcttcaatat gaattccatt tccggtatca ctcactgaaa
     9361 taatagttgt tataggtgtt tcaacccatt ctatcattat tttatcgttc tcttttgtat
     9421 tatctaatgc attttttata aggttgctta aagcttctga tatccattca taatcgcaaa
     9481 acaagtttgt attttctaga cctcttatag agatactctt tccttctttt tctgctctag
     9541 tttcaaaact atataaaatt ttagtaatca acttttttac gttaacacta gctttgttaa
     9601 gaattattgt ctttgaatct atctttgtaa tctttaacaa gttttgtatc aatgattcta
     9661 tacgctctaa ttcattttga ctcttctgca caaaattctc aacaatttca ctattatgac
     9721 tctcttcctg caatatttga ttatacatca tcaatgctgt aagtggagtt tttagttgat
     9781 gtgatatatc tgaaattgta tttttaagaa attctttatt ctgcttttct ttcaatatat
     9841 gtgtgtttag agatgttgac atagcatcta ttgaaccaaa taactttgat aaactacctt
     9901 cttcataagt atttaaccgt acatttatat ttccattcat aaatgaatca attttataac
     9961 tagcttcttc tatcttgttc tgttgcctaa aaaaatacac atacaatata aaaataatca
    10021 tagtaaagaa cacaaataaa tacaaaaaca tagtttttat atatttacct tgaaacaatt
    10081 tcacacttgg cataaaacta tcatctgtac caaagtcata tctagctgta tttaagattt
    10141 cttttccttt taaagtatat ttatcttcat tagtagtaaa tactgctggt atttttgatt
    10201 tatcaacacc catactattt aagtatccag ctatattata atcatgttct aacagtgcac
    10261 ttttataatc attagttata tttataacta ctatttgacc tactactata aacatcaata
    10321 aaacggatat aatcaccaaa aaaaatattt ttatgtcttt attagaaaat accttcatat
    10381 taacaccctc actttataac attccatttg taacccattc ttctaactgt taatagattt
    10441 tttggatttt caggattatc ttcaatctta tttcttaacc ttcttacata tactgacaga
    10501 gtattatcat ctacaaaact accatttcca tcccataact tatccaatat attttttctt
    10561 gtaagtacaa tatttttatt tttcataagt aagcataata atttatattc tgctgtagta
    10621 agttcaagct caatttcatt tttaaaaaca cgattttcaa gtaatttaac agtaatatta
    10681 tttgatttta gttcagttac attttcactt gtaaaattat aacgtctaag taaagctttt
    10741 attcttgaaa ttaactcatt taatttaaaa ggttttgtta tgtaatcatc tccacccata
    10801 tcaagtccca tcactacatt tacttcttca tcagaagcag ttaaaaatat gattggtaca
    10861 ttagaagctt ctcttaccct tttacaaata tcaaaaccat caccatcagg taaggataca
    10921 tccaaaagca gtaaatcata atttttttct ttataaattt gcaatgcttc ttctactgtt
    10981 cttgctacat caacactaaa tccattcttt tgtatagaat attctaatcc atatatcaaa
    11041 cttaaatcat cttcaaccaa caatatacta ctcatctcaa tcacttccat ttttttattt
    11101 tttactttat atatattata tacctaactt attatatttt tactctttta aaaatacaat
    11161 accttgaaat caatgattta aatattttaa aattattttt atatgatttt atatagttaa
    11221 tttaatactt tgaaatctta ataataaaaa aagcttaaaa tacactaagt attctaagct
    11281 ttattcaatc acattttata tatttatatt atttattttt ttaaaatact attttcaata
    11341 ttgcctctat taaacatttc attatcatta tctaaagaga tatcacttat atttctaaca
    11401 gttgttgaat agttaccact aagttctatc tcactgaaat gcatatgcac tatcttttgt
    11461 attacaaatc ccattgatgc aagtaaaata gcaaatatta tatactgact aaagttactc
    11521 atataataat ttataatggt atattcattt cctttgactg ttaaatgttt tgtagatagc
    11581 atagtatata tatattcagt acaatttata aatacatata taccataaat agctaacact
    11641 acagataaac aatacattgt gattgaaatc tttgatattt ttttaaactt actatcttta
    11701 caacacttaa tatttgttat ttatcataaa caaggttatt gtattatcaa taatttttag
    11761 gttacactta ttattagtct tgtgttaaaa cgacagtaga cttaagagta gagttttatt
    11821 aagccaatat ttatatttgc tatacattgt acccctatta catcatacat ataacttgga
    11881 taacttattt tatttttatt tctaaattat ttaaaatcaa tatctacacc attatcgata
    11941 attgcaatta aattcatata aaacctcttt aatttatagg ctgttcacta taaagtcttt
    12001 atttcttcca taaaattttc attttgaatt ttattcaatg tttttagtgt agttatcatg
    12061 attatagata ctaaaataaa tactattagt ataatagccc ctataaattt ccaatttatt
    12121 aaattgatac tttcaaaagg ttttattatt atacttctta caattaaaat aggtattata
    12181 gatatacaaa agcttactat aatatatata taactttcca atttaacaat tttatttatt
    12241 tcatgttttg atattcctat tcctctaaat agtgcaaatt ccctacttct gctattaata
    12301 ctagtaacta tagtattgca acaattaaat aaagataata taactataat tccagttact
    12361 aataccataa ttagtgtttt ttgattatca attttttcat attcagctgt ttcagctttg
    12421 tatggaatta aaattccttt atttgttttt tctgttattt tgtttaactc actttcaaca
    12481 tatttatcat tggctagttt actcgtccat atgtcaaatc tttcataaga tgatattcct
    12541 gtaaacttat taaattgatt tgttgatatt actccgccaa attcagctcc cacttgtgag
    12601 gttactggta atgtagttat aattccacat actataaatt cttgagatat tgttttggta
    12661 aaattatttg tttttgaatc tataatatca aagtatactt ttattctatc tccttttttt
    12721 atattaagcg aatcactgga tttctttgtt atatacagat atggttgttt actaaattta
    12781 tctatatttt cctttccatt tatcaagaaa tcctctagta tatctgtatc ttctattcct
    12841 aaaaattcaa aagaattatt atatacctta attccatctt ttacttctga tctagcttca
    12901 atattatctt tatttttttt ccaataatca gagttcttat ttatactttt atcatcaatt
    12961 tttatattaa accacttatg cctatatgag tttacttttg ttacaccatc aattgccttt
    13021 atctcttgta gtacattttt atcataacct ttattatttg aacgtatagt acttatatca
    13081 atattatgta ataaataatc tccttgtatc caatatttta atacaccatc acttaaaaag
    13141 ttactagtag actttgtgta tattgttgcc attaagaaaa atataattac cattgatgct
    13201 atagataaat ttgtacgttt cttatttctt tgtatatttt ttgatgctat gttaccatag
    13261 tctttgaaaa gttttatcat aaatttattt ataaaggaat tttgttttat atccactttt
    13321 tcattatttc ttcttataca aatcattgga tcagttttaa ctgataacat aagaggtttt
    13381 gaaactgata ttattatagt tcctaaactt aataatatag aaattatgta tgtgtttata
    13441 tagttctggt tttttataga atttatatag ttgacattgt atattactcc tataacatac
    13501 tcccttgata aactagctat tatcccacct aaaattagac ctatagaaat acttataata
    13561 gagtatatag atacttctaa aatcattatt ttaaaaatat catttggatt aaaccctaaa
    13621 gccctaaata tcccaaaatc ctttattcta tttgatacta atatataaaa aatattataa
    13681 attaataata tagttgctag tgctagtact atattaacta ttatataagg tatttttaaa
    13741 ttcatactat cagatatagc tattaataat tcattattaa aataaatgtt attctcattc
    13801 aaatttccat ttttaataag tttttgtgat tgtctctgta tatttttttc ctttttaaaa
    13861 gtaaacataa cttgatcata tttattttta gtaggaatat tcttgatagc acaatcgtta
    13921 cttataaggc caattgatgt tccttgagct tttaaaatag gattactatt taagatacca
    13981 gttattttaa attctttttc cccagtatat aaatcttctt gatctatccc ttgtcttcta
    14041 taatctaatg ttatcctttt tcctataggg ttttttatct ttttttgttt aatataccat
    14101 gaatctatag cgatttcatt ttcagctact ggtaatttac ctttttctag tgtgtaaata
    14161 tcatttagtt cttcagcaac tttatcctga gatagaattt gtagtgtttg acctttacca
    14221 atatcactaa ttcctaaaag cattgatttt ccaactttat ctatacttgt gttattttct
    14281 atatatttta tttcttcgtc ttttggattg atatattgtg catgttttaa tgtaaattca
    14341 tttttagctt gatttatcat taagtttgtc gcatcttcac ttattatatt cataattaaa
    14401 aacaacgctg ttgaaattac aatacttaca attattaata atgtattttt cttatttccc
    14461 attatatatt tttttacaat ataattaagt ttcataaatc tacctactct cttataaatc
    14521 catcttctat ttctataact ctatcagcat attttgttat atctatatta tgtgttatca
    14581 ttattattgt ctgattatat tttttttgag attcttttaa taaacttaat acatctgact
    14641 ctgtctttgt gtctaaattt ccagtaggtt catcagcaaa tacaattgat ggtttattta
    14701 caagagccct tgctattgct actctttgtt gttgcccacc tgataattga tttggcaaat
    14761 acattcttct atcttttatt cctagtaact ccatcaattc attaatataa gcttcatctt
    14821 tattttttat atttatagtt ggtagtttta tattttcttc aacagttaaa actggaatca
    14881 aattatatga ttgaaatatt aatcccaact tttctttacg taatgcagat cttttctttt
    14941 catcaagaga tgatatattt atattatcaa taatcacatc accatttgtt ggagtttcaa
    15001 gtcctgccat tatgtgtagt aaagtacttt tcccacttcc acttttacct actatcacaa
    15061 caaattctcc actatttatt tttagattta tttgacttaa cgctttcacc tctgtttccg
    15121 atttaccata tattttattt aggtttttta tctctaaaat actcataatt tactccttgt
    15181 cttacatgtt atttacactt ataaattcga tttttgctat ggattttgca atctctatct
    15241 atacttattg atatgtagca acagaaacta tttttataat tttcaaaaaa ttcttccgcc
    15301 tattcactcc atatagtgcc tttagttatc ataccatatc ttataattga attttatttc
    15361 ttatatctct tttaccaacc tttttattta ggaaaagttc ttcaccatga aattgtacag
    15421 tatataccta tttctttgat tatataatta agctttttat ttagtaacct attttctaca
    15481 tcctaactta tatttcctgt ttcctcattt tcatatttat aacaatagtt acaatctaaa
    15541 ttactacttt tagttaccca gactttactt tacatgcttt gcctctaaac tgctgatgtt
    15601 aatatcttct taattttttc acacctatct tcaaatataa aatctttatc taaaagatta
    15661 ttgtattcag ctagacataa ccaacaaaat gatttcatta aatattcttt acattcatta
    15721 ttacataact ttaagttgaa aaactcatta tatccaaaat tcgttgatac ccatattatt
    15781 aatataactt acaatgtctt tttgaatatc taaaaaaata cttttataac ttattttata
    15841 tatcatacac tatattattg ctttcataat ctataaaggt ttcatatgat acaaacttct
    15901 aaggtgggat ttttggtctt taataacgat tataataata tttatttata tttc
//
LOCUS       DAMJYR010000087        15909 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.089, whole genome shotgun sequence.
ACCESSION   DAMJYR010000087 DAMJYR010000000
VERSION     DAMJYR010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15909)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15909)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15909
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.089"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            149..1054
                     /locus_tag="PC471_002634"
     CDS             149..1054
                     /locus_tag="PC471_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HDF2789751.1"
                     /translation="MKNSNFNILAFDGGGLKGALSISILERIVKEYPNLLNNINMFGG
                     TSTGSLIALGLAYGVSPKEIKELYSIENSKYIFNKSYAEILRPKYENKNLKEVLLSIF
                     PEELELKDLNKLVMIPSFYIGNEENAWKPVFYNNMPNSFTKTSKVVDVAMASSAAPVF
                     FPTYNRHVDGGIIATDPSLACIIHAIDSGFKLKNTRLFSIGTGYVYNSIKADTTEWGA
                     IDWIINKEPDLPIISITLEGNSQMSQLFSQKLLGDNYYRLNPKMEKDVAMDDCDALEY
                     LMSLGENCDIKDAFGWIKKKSFYRN"
     gene            1178..2131
                     /gene="msrA"
                     /locus_tag="PC471_002635"
     CDS             1178..2131
                     /gene="msrA"
                     /locus_tag="PC471_002635"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HDF2789752.1"
                     /translation="MIKLATFSGGCFWCMVKPFDKYDGVIKVVSGYTGGHVENPTYQE
                     VCSGTTGHLEAIQITYDDDIISYDELLNIFWKQINPTDDGGQFGDRGTQYKTAIFYHD
                     EEQKNIALESREHLENSKIFDSKIVTDIIPATKFYEAEENHQDYYKKRPLNYNMYYKG
                     SGRYNFVKSHWDGNNINREELKKTLTPIQFEVTQNDMTEVPFENEYYDNNKEGIYVDI
                     VSGEVLFSSKDKFDSGCGWPSFSKPVIDKSIIERSDFSQGMYRVEVRSAKGNSHLGHV
                     FDDGPKELGGLRYCINSASLKFIPRENMEALGYGEYLKLFE"
     gene            2284..3414
                     /locus_tag="PC471_002636"
     CDS             2284..3414
                     /locus_tag="PC471_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF2789753.1"
                     /translation="MNLKDFNGKRLKSARIFRAKTIEQLSKETKINKKDLKAFEENKY
                     VPNIENTLKLSNILNFPKEYFYKNENINIVVEDSHFNPQSKLPRVEEISYREKIIIIH
                     KIYSFMENYIKFPKENLPNRKVMSDININDIESLAYKTREFWDLNSTPIVNMVSLLES
                     KGIIISGMNVDRKGATIFTQKQRISKESKYLISLGNDKKSASIRNYTLACELGYIISS
                     ELRIPSKQFSEDEYACAFLLPKESFLKDLTHPEDLDYYVELKKKWIVPISAMIFRAYN
                     LEKINYKKYNYLMNEMDKKGWLIEEPLDKMKGSSPTYLKRAVELLIENKIMSVNSIVS
                     SLEEFGINLYPEDLELLMGLKKGLLSQEVNKKSKVIKFDGKK"
     gene            complement(3531..4499)
                     /locus_tag="PC471_002637"
     CDS             complement(3531..4499)
                     /locus_tag="PC471_002637"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HDF2789754.1"
                     /translation="MAIKPRKISIVGSGHVGSHCGFSLITQGVCDELFMIDIDESKSK
                     AQALDLADAVSYLPHKVHIEKGTFSDCKDSDIVVISVADSSEGPLRRQNTTRLDLLRP
                     TIGMIKSIVKPIVDSGFDGIFVVISNPVDVVTNYIWEKSGFPKNKVIGTGTALDSTRL
                     RRILSEETGIAQQSIQAYSMGEHGDSQMVPWSHVSIGGKPILDMIKDNPSTYSNLDLP
                     SIVEKNKKTGISIINGKDCTEFGIGTALVEIVKAILHNEKKVLPVSTLLEGQYNERNV
                     FAGVPCVIGKDGIEEIIEINMTEYEQNEFNKSCSVLREYIALSKTL"
     gene            4834..5022
                     /locus_tag="PC471_002638"
     CDS             4834..5022
                     /locus_tag="PC471_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2789755.1"
                     /translation="MNYHTKEELVEALRVVSSSIINCGKGQKKFSEETSHHTRFKNII
                     EVMYISKSLIMDEISKRD"
     gene            5282..6481
                     /locus_tag="PC471_002639"
     CDS             5282..6481
                     /locus_tag="PC471_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanyl-tRNA editing protein AlaX-L"
                     /protein_id="HDF2789756.1"
                     /translation="MEKLYYTNQYIRDFTAEIIEIKEVENKYHVLLDKTAFFPGGGGQ
                     FGDLGKIENDDVINVYEDGKKIYHVLDKKPIKIHKVKCSIDWDRREDGMHQHFGQHVL
                     SGCFFKTFNANTTGFHLGNEYSTVDIEGNLEEDKIREIELFANEIIRQNIQVETLLPT
                     KKELKKIWLRRDLPNTDEEIRVIKIGDLDTNACCGVHPKSTGELRMIKIKKWEKHKSS
                     TRIEFLAGKRAVDEVLKRDIYLTKICRYLSCSEEDAINGIVNLNSKVEEALIKKRRLE
                     EVVSTYQVKEMIEKANKVGNISVIKKIYDDEDLKYVNKIANKIAEYENSIALLAVKFN
                     DKINLVFACSKNLKDINMGVLLKDAINLIDGKGGGSKVLAQGGGKNNGNLEATLDYAF
                     MKIEKTL"
     gene            6533..7036
                     /locus_tag="PC471_002640"
     CDS             6533..7036
                     /locus_tag="PC471_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDF2789757.1"
                     /translation="MEQQKDYIELYENEELIEIREARMDDLDTIAKFNYNLAKETEGK
                     ELDMDVLTKGVKALLLDERKGKYHVYTVFDKVVAQIMYTYEWSDWRNGNFLWIQSVYV
                     DKEYRRKGIFNYLFNYIKNICDKDENIVGMRLYVEKENINAKATYESLNMYECDYNMY
                     EYEVVHS"
     gene            7143..7460
                     /locus_tag="PC471_002641"
     CDS             7143..7460
                     /locus_tag="PC471_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2789758.1"
                     /translation="MGVLKGMFYVFLFLDLVSIFCFFFNKGKIASNKIVFNAIGVLTF
                     VLCFMLFSYYPNNNLIGKFIASLFFIFGVAGVMLKEKNFLYARLLLTVVIVFSTLRLF
                     VIQ"
     gene            7618..8577
                     /locus_tag="PC471_002642"
     CDS             7618..8577
                     /locus_tag="PC471_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HDF2789759.1"
                     /translation="MIESKKSEKLLLSIIYIIFLVYILMIIRIILFRDVPIYAIFKGT
                     FRNVNLIPFYTIYQFIVDSNLDFMKATVNIIENIGIFIPMGIFLPIVCKNLNKKTIII
                     VIILVSLAFELTQYIFALGSSDIDDVILNSLGGIIGINIYINMNKLFPNDINRFKAII
                     GTSLVLGVIGLGVISKNYHNLLTFKFRPDRKISKILIEENREIIKDINKDTVDIVGTF
                     ESFKKGIITIKAGSNNKVKRPENLIDSDGNIRIYLNENTKLVSYIITKESKMDIVKYE
                     EFDVKNLDLLRRYDTINVWIDKENQPKDKHGIMASKLLIGLYE"
     gene            8692..9477
                     /locus_tag="PC471_002643"
     CDS             8692..9477
                     /locus_tag="PC471_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter permease"
                     /protein_id="HDF2789760.1"
                     /translation="MEIIKYILYFFIYSFLGWTVESIGCSIASKRIINRGFLNGPICP
                     VYGFGAVIVISLLGRFNNVVIVFLLGMILTTILEYFTGFILETLFHAKWWDYSDRKFN
                     IKGRVCLKNAIYFGVMSVLIIRFIHPFIKYFVSIIPYRILISMAIITTLWTILDLIVT
                     IITLKKLDIKLNLLDDIITDLNDINVKLDKFDRGEIQALFKTINNDELEVREKINKIN
                     SKLDHIESNIILQKRVIKAFPHIKHKKQQEQLEHFKKIINEKR"
     gene            9769..11088
                     /locus_tag="PC471_002644"
     CDS             9769..11088
                     /locus_tag="PC471_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HDF2789761.1"
                     /translation="MSEITSQMISTEEKKYVAKTQKIPYYPVAFKSGDGAMLYDYEGN
                     EYVDFLASAGSANVGHGNKEISQAVKEQMDDITQYTLAYFHSDPPVKLAEKLVEIAPG
                     DNDKKVLYSATGSACIDAAIKLARGYTGRTKIISMCESYHGSTYGAISISALSTNMRR
                     KMGPLLPEVYHFHYPDKNRTAKECLDEIEYAFAHYLPAEEVAAIFIEPIAGDAGIIVP
                     PVEWVQGLSKICKENGILLVSDEIQQGMGRTGKWFGIENFGVEADLIVLGKSVGGGLP
                     LGAVVGRTEIMQSLDAPAHLFTLAGNTTVCVAALKSIEIIEKENLLQKSIEMGDYIKA
                     GFEKLKEKYDIIGEIRGIGLSIGVDIVKGKGSNEKHPDATAKICYRCIQTGLIMIFLG
                     QSTLRVQPPLVITKEQVDKAMNIIDSAIDDYLNGRIGDEVYEVTQGW"
     gene            complement(11163..11591)
                     /locus_tag="PC471_002645"
     CDS             complement(11163..11591)
                     /locus_tag="PC471_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2789762.1"
                     /translation="MKDNNNTNKTIQFGEKKASLENNIQKNKNIKLDNTHKVIPYPKN
                     RKKDDKKKGNKKKNTNTSKVNKNNYGHSFFNNKYKYKRKPYVIALYTVILILLAILII
                     TKLTDSTNKGNYSRNTVSDTHKNDIRNIVFNESNNFLICG"
     gene            complement(11682..13058)
                     /gene="hydG"
                     /locus_tag="PC471_002646"
     CDS             complement(11682..13058)
                     /gene="hydG"
                     /locus_tag="PC471_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydG"
                     /protein_id="HDF2789763.1"
                     /translation="MFINHELINSLLEDAKNSTSDDIEKVLDKADRREKLSYKDIATL
                     LEVEDKKQLDRLFSIAGQIKNEIYGNRVVLFAPLYVSNYCVNECVYCGFSKCNKFKRK
                     KLTMEEIKEEVKILEKMGHKRLALEAGEDPKNCDINYILDCLDAIYSTYNENGNIRRV
                     NVNIAATSVDEYKLLKEKGIGTYILFQETYHKPTFIKMHGQSIKNDYYYHLTAFDRAM
                     EAGVDDVGAGVLFGLSDPKFEVLGLMMHNEHLEEKFGVGFHTISFPRLKKAEGMSLED
                     FPHLVSDDMFKKIVAITRLAVPFTGIIMSTRETAEMRNELLKYGVSQISAGSLTGVGG
                     YKAYEDGDNTEQFEVGDHRSPVEVLKELITDGYIPSYCTACYRKGRTGDRFMSLAKSR
                     QIHNVCTPNALTTLNEFLIDYGDEELKIMGKKLIAEEIGKIEREDIRNIVSNNMTALE
                     RGERDLYL"
     gene            complement(13059..14144)
                     /gene="hydE"
                     /locus_tag="PC471_002647"
     CDS             complement(13059..14144)
                     /gene="hydE"
                     /locus_tag="PC471_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydE"
                     /protein_id="HDF2789764.1"
                     /translation="MTKNKIKYFIDKLYSINSLSFDELLYLIENIDNEEYNFEQNLRN
                     YLYKQASDMRNKHYGNRVYLRGLIELTNYCKNNCYYCGIQSSNKNIIRYRLSLDEILE
                     CCDIGYKIGYRTFVLQGGEDAYFTDDKICEIVSSIKNKYEDCAITLSLGEKSYDSYKK
                     YFLCGADRYLLRHETATDSHYKKLHPPNIELRTRKECLKNLKEIGYQVGAGFMVESPF
                     QNNEDLVRDLLYLKELNPHMVGIGPFIPHHDTIFKDYKHGDLEKTLLMLSITRLLLPK
                     VLLPATTALASINPSGRNAGLLAGCNVIMPNLSPQEFRKQYSLYDNKAFTGQEACEYH
                     QSLEENIKSLGLEVDYSRGDNIEWRRL"
     gene            complement(14180..15388)
                     /gene="hydF"
                     /locus_tag="PC471_002648"
     CDS             complement(14180..15388)
                     /gene="hydF"
                     /locus_tag="PC471_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster maturation GTPase
                     HydF"
                     /protein_id="HDF2789765.1"
                     /translation="MSLNSTPQSVRVHIGLFGKRNAGKSSIINAITNQSAAIVSDIAG
                     TTTDPVFRPMEILPIGPCVLIDTAGLDDVGELGELRIGKSLDVLEKTDIALLVVDCQI
                     GISQEDLSLIEKFNDKNIPHILILNKIDTIKNQSEILNLTKNKVKCPVVSVSSTDKIG
                     IENLKNEIIKVLPKDSTEFKLVSDLIEPNDLVVLVVPIDKAAPKGRLILPQQQVIRDI
                     LDNGAISIVTKEDSLKETLSNLGKKPKLVITDSQVFPQVDKDTQKDIPLTSFSILFAR
                     QKGDLKELINGAYALENLKDGDKILMAEGCTHHRQTDDIGTVKIPNMIRKKTGKNITF
                     EFSSGVSFTEDINKYALVVHCGACMMNRAGMLSRIEKAKSFNVPIVNYGVLIAYVKGI
                     LERSLELFNY"
     gene            complement(15403..15654)
                     /locus_tag="PC471_002649"
     CDS             complement(15403..15654)
                     /locus_tag="PC471_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003361768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-only hydrogenase system regulator"
                     /protein_id="HDF2789766.1"
                     /translation="MKKVAVISAILEEPKECQFKFNEVVSGFKGIIKGRMGIPCEEEG
                     VSIVCIAVVGEMDTINSLTGKLGNIPHVSVKTSISKKEV"
BASE COUNT         6001 a         1866 c         2501 g         5541 t
ORIGIN      
        1 ttattttaag tattaaaggt ttgaatgtaa cttaaatatt aaaagaggaa cattgtaata
       61 tgtaacttgc ataaaatata tatactatag tgaaattatt ttgattaaaa tttaaaatat
      121 aaggtgtttt atgtgggggt atatgtatat gaaaaattct aattttaata tattggcatt
      181 tgatggtggt ggattaaaag gggcattgag tatatccata cttgagagga tagtaaaaga
      241 gtatccaaat ctattaaaca atataaatat gtttggaggt acatctacag ggagtctaat
      301 tgcactaggg cttgcgtatg gtgtaagccc taaagagata aaagaacttt actcaattga
      361 aaattcaaag tatattttta ataaaagtta tgcagaaata ctcagaccaa agtatgaaaa
      421 caaaaactta aaagaagttt tattaagtat atttccagag gaactagaat taaaagattt
      481 aaataaatta gtaatgatac caagttttta tataggaaat gaggaaaatg catggaaacc
      541 agtgttttat aacaatatgc caaattcttt tacaaagact agcaaagttg tagatgtagc
      601 aatggcaagt agtgctgctc cggtattttt tccaacatat aatcgtcatg ttgatggagg
      661 aataatagca actgacccga gtttagcgtg tataattcat gccattgaca gtggatttaa
      721 acttaagaat acaagacttt tttcaatagg gactggatat gtatataaca gtataaaggc
      781 agatactacc gaatggggag ctattgactg gataattaat aaagaacccg atttaccaat
      841 tatatctatt accttagaag gaaattctca aatgagtcag ttattttctc aaaaacttct
      901 tggagataat tactatagat taaatcctaa gatggaaaaa gatgtagcta tggatgattg
      961 tgatgcatta gaatatctaa tgtctttagg agaaaattgt gatataaaag atgcttttgg
     1021 atggataaag aaaaagagct tttatagaaa ttagattcaa tttgcaattg tatttttaca
     1081 catgatttca ttgagtaggt aaaaataata aaatgcagat tattaagtta cttcttaggg
     1141 aataaataaa gtataattta tttaaggagt gatgaaaatg ataaaacttg ctactttttc
     1201 aggtggatgt ttttggtgta tggtaaaacc ttttgataag tatgatggag ttattaaagt
     1261 agtttctgga tatacaggtg gtcatgttga aaaccctaca tatcaagagg tatgctcagg
     1321 aactactggt catttagaag caattcaaat aacatatgat gatgatataa taagttatga
     1381 tgaacttcta aatatcttct ggaaacagat taatcctaca gatgatggtg gacaatttgg
     1441 tgatagggga actcaatata agactgctat attttatcat gatgaagaac aaaaaaatat
     1501 agctttagag tcaagagaac atctggaaaa tagtaaaata tttgatagta aaatagtgac
     1561 tgatataatt ccagcaacta aattctacga agcagaagaa aatcatcagg attattataa
     1621 gaaaagacca ttaaactaca atatgtatta taaagggtct ggaagatata attttgtaaa
     1681 aagccattgg gatggaaaca atataaatag ggaagagtta aaaaagactc taactcctat
     1741 acaatttgaa gtcactcaaa atgatatgac agaagtacct tttgaaaatg aatattatga
     1801 caataacaaa gagggaattt atgttgatat tgtaagtgga gaagtgttat tttcttcaaa
     1861 agataaattt gattctggat gtgggtggcc aagtttttca aagccagtta tagataaatc
     1921 tatcattgag agaagtgatt tctcacaagg tatgtacaga gttgaagtaa gaagtgctaa
     1981 aggaaattct catttaggtc atgtatttga tgatggtcca aaagaacttg gaggtttaag
     2041 atattgtata aattctgctt ctcttaaatt tataccaaga gaaaatatgg aagctttagg
     2101 atatggagaa tatttaaaac tttttgagta attaggaata tttattataa acttatctaa
     2161 aaggtgaaaa tataaaaaaa tattttcacc ttttgttttt ttgtcaataa tatgataaat
     2221 tattaattgt aatcttattt ataccaatag attaagaatt taattttgaa agagagaaaa
     2281 caaatgaatt taaaagactt taatggaaag agattaaaat cagctagaat ttttagagca
     2341 aagacgatag aacagttgtc taaagaaact aaaataaata aaaaagattt aaaagctttt
     2401 gaggaaaata agtatgtacc aaatatagaa aatacattaa aattatctaa tatattaaat
     2461 tttccaaaag agtattttta taaaaatgaa aatataaata tcgttgtaga ggatagccac
     2521 tttaaccctc aatctaaact acctagagta gaagagattt cttatagaga aaaaataatt
     2581 ataatacata aaatatattc atttatggag aattatatta agtttccaaa agaaaacttg
     2641 ccaaatagaa aagtaatgtc agatattaat ataaatgata tagaatctct tgcatataag
     2701 actagagagt tttgggattt aaatagtact ccaatagtaa atatggtaag tttgttagag
     2761 tctaaaggga ttataatatc aggtatgaat gtagatagaa aaggggcaac tatctttact
     2821 caaaaacaga gaattagtaa agaatctaaa tatctaatat ctctgggtaa tgacaaaaag
     2881 tcagcatcaa ttagaaatta tactttggca tgtgagcttg gatatataat atcaagtgaa
     2941 ttaagaatac caagtaagca attttcagaa gatgagtatg cctgtgcatt tttattgcca
     3001 aaagagagtt ttttaaaaga tttgactcat ccagaagatt tagattatta tgtagaacta
     3061 aagaaaaaat ggattgtacc tatatctgct atgattttta gagcatataa tctagaaaag
     3121 ataaattaca aaaaatacaa ttatcttatg aatgaaatgg ataaaaaagg ctggttgata
     3181 gaagaaccac tagacaaaat gaaaggttca agcccaacgt atttaaaaag agcagttgaa
     3241 ttattgatag aaaataaaat aatgagtgta aatagtatag tatcaagttt agaggaattt
     3301 ggaataaatc tttatccaga agacttagaa ttattgatgg gattgaaaaa aggattgtta
     3361 tctcaagagg taaataaaaa aagtaaagtt ataaaatttg atggtaaaaa ataggtttat
     3421 attttagtat taaaattaaa agcaggtaca aatgatttaa acattaactt agttaaaggt
     3481 cttttcaata agtcatttac ctgcttttag tgtaagaaag aatattgaat ctaaagtgtt
     3541 tttgaaagtg caatatattc ccttaaaaca gaacaagatt tattaaattc attttgctca
     3601 tattcagtca tattgatttc aataatttct tcaataccat cttttcctat aacacatgga
     3661 acacctgcaa atacatttct ttcgttatat tgaccttcta gtaatgtaga aactggaaga
     3721 acttttttct cattgtgtag tatagctttt acaatttcta ctaaagcagt acctatacca
     3781 aattctgtac aatctttacc atttattata cttatccctg tttttttatt tttttcaact
     3841 attgatggta agtctaaatt gctataagta cttgggttgt cttttatcat gtctaagatt
     3901 ggtttaccac caattgaaac atgagaccaa ggaaccattt gagaatctcc atgttcaccc
     3961 atagaataag cttgaattga ttgttgagcg attccagttt cttcagataa aatccttcta
     4021 agtcttgttg aatctaaagc tgttccagtt ccaatgactt tgtttttagg aaagccagat
     4081 ttttcccaaa tataatttgt aactacatca acaggattag atatgactac aaatatacca
     4141 tcaaagccag aatcaactat gggttttact atagatttta tcattccaat agttggtctt
     4201 agtaaatcta atctagttgt gttttgtcta cgtaaaggcc cttctgaaga gtctgcaaca
     4261 cttatcacta ctatgtcaga atctttacag tcagaaaaag ttcctttttc tatatgtact
     4321 ttatgtggca gatatgaaac tgcatcagct aaatctaaag cttgagcttt tgatttagat
     4381 tcatctatat caatcataaa gagttcatca caaactcctt gagttattaa actaaatcca
     4441 caatgagaac caacatgccc tgaacctaca atagatattt ttcttggctt tattgccatt
     4501 ttaaatcact ccccctaaaa taacgataca ttgaaaatta tagatgttat taaaagctgt
     4561 ttttggatat tttaatattc tattatttca gaaaatttat ttaatatgat tttatactaa
     4621 aatttttaaa aaagcaatgt taatatactc tctattagtg aatattaaag tattatatgt
     4681 atattaataa ttattaaaat atgtaatgtc actgatagtt agaatattta agtgctaaaa
     4741 gatgtgtata aataataatg atgtcagttt ataatgatat taaattgaga tatactgttt
     4801 ctctaaatta aaaaataaag gagtaaatgt gttatgaatt atcatacaaa agaagaattg
     4861 gtggaagcac tgagagtagt atcttcaagt attattaatt gtggaaaagg acagaagaaa
     4921 ttttctgagg agacatctca tcatacacgt tttaagaata ttatagaagt aatgtatatt
     4981 tcaaaatcat taataatgga tgaaattagt aaaagagatt gaagcttatt ttgcaattta
     5041 acatgtttta atatacattt tattatataa gaaaataagt aaacatacat gttataaaat
     5101 attgaaataa tatacaatta taaatttgtt aacattaata tctatatgtt aaaatatagt
     5161 agaagatgtt agtaaataat aggatgagtt ctattttaat acaattaaat taagcttagt
     5221 ttgaaatttg tatctaaaaa gtaaagtaat ctagaattaa gaaaatggag atagaattat
     5281 aatggaaaaa ttatattata caaatcagta tataagagat tttacagcag aaataattga
     5341 aataaaagaa gtagaaaata agtatcatgt tttattagat aagacagctt tttttcctgg
     5401 tggaggaggt cagtttggag acctgggaaa aatagagaat gatgatgtaa taaatgtgta
     5461 tgaagatgga aaaaaaatat accatgtatt agataaaaaa cctataaaga tacataaggt
     5521 aaagtgtagt attgattggg atagaagaga agatggaatg catcaacatt ttggacaaca
     5581 tgtattgtct ggatgttttt ttaaaacttt taatgcaaat acgacaggtt ttcatttggg
     5641 aaatgagtat agtacagtag atatagaagg taatttagaa gaggataaaa ttagagagat
     5701 agagttgttt gccaatgaaa ttattcgtca aaatatacaa gtagagacat tacttccaac
     5761 gaaaaaagaa ctaaaaaaga tttggttaag acgtgatttg ccaaatactg atgaagagat
     5821 aagagtaata aaaataggtg atttagatac gaatgcatgt tgtggagtgc atccaaaatc
     5881 aactggtgaa ttgagaatga taaaaataaa gaaatgggaa aaacataagt caagtacaag
     5941 aatagagttt ctagcaggta aaagggcagt ggatgaagta ttgaagaggg atatatattt
     6001 gactaaaata tgtagatatt taagctgtag tgaggaagat gctataaatg gtattgtaaa
     6061 tctaaatagt aaagtggaag aggctttaat taaaaaaaga aggcttgaag aagtagtatc
     6121 tacatatcag gtcaaagaaa tgattgaaaa agcaaataag gttggcaata tttcggtcat
     6181 taaaaaaatt tatgatgatg aagatttgaa atatgtaaat aagatagcaa ataaaattgc
     6241 agaatatgaa aatagtattg cattattagc tgttaaattt aatgataaaa taaatttagt
     6301 ttttgcatgt tctaaaaatt taaaagatat taatatggga gttttattaa aggatgcaat
     6361 aaatcttata gatggcaagg gtggtggaag taaagtatta gctcaaggag gaggtaagaa
     6421 taatggaaat ttagaggcta cacttgatta tgcatttatg aagatagaaa aaactctata
     6481 aatatattat ataaaaaacc ttagtagata aaatagatat ggaggaataa gtatggagca
     6541 acaaaaggac tacatagagt tatatgaaaa tgaagaacta atagagataa gagaagcacg
     6601 tatggatgat ttggatacaa tagcaaaatt taattataat ttggctaaag aaactgaagg
     6661 aaaagaactt gatatggatg ttttaactaa aggtgtaaaa gcattgttat tagatgagag
     6721 aaaaggaaaa tatcatgtat atacagtatt tgataaagtt gttgcacaga ttatgtatac
     6781 atatgagtgg agtgattgga gaaatggaaa tttcctttgg attcagagtg tatatgtaga
     6841 taaggaatat agaagaaaag gtatctttaa ttatttattt aattatataa aaaatatatg
     6901 tgataaagat gaaaatatag ttggaatgag attatatgta gaaaaagaaa acataaatgc
     6961 aaaagcaaca tatgagtctt taaatatgta tgagtgtgat tataatatgt atgaatacga
     7021 agtagtacat tcgtaatata aatttgtaat actgttatac tgatgttata aagtattgat
     7081 tatattgacg attatatgaa ataaactgga tattataatt agaattttta gggggtataa
     7141 aaatgggcgt tttaaaagga atgttttatg tatttttatt tttagattta gtgtcaatat
     7201 tctgtttctt tttcaataaa ggaaaaatag catctaataa aattgtgttt aatgcaatag
     7261 gagtacttac atttgtactt tgttttatgc tttttagtta ttatcctaat aataatctta
     7321 ttggaaaatt tattgcttct ctatttttta tatttggagt tgcaggagtt atgttgaaag
     7381 aaaaaaattt tttatatgct agacttcttt taactgtagt tatagttttt tctacattaa
     7441 gattgttcgt tattcaataa aatgtatttg tataaaaatt tattaaagtc tacacattta
     7501 agtgtaggct tttttgattt tttgtttgaa aattaaggaa attttaagga aattttaaaa
     7561 tcgtcttaaa tatttattga tatacttaaa atattaatag cgaaaggaga cttatcagtg
     7621 atagaaagta aaaagtcaga aaaattgtta ctgtcaatta tatacataat atttttggta
     7681 tatattttaa tgattataag aataatttta tttagagatg taccaattta cgctattttc
     7741 aagggaacgt ttaggaatgt aaatttaatt ccattttata caatatatca atttatagta
     7801 gatagtaatc ttgactttat gaaagctact gtaaatatta tagaaaatat aggtatattt
     7861 attcctatgg gaattttttt acctatagta tgtaaaaatc taaataaaaa aactataatt
     7921 atagtaatta tacttgttag cctagcattt gaattaacac aatatatttt tgctcttgga
     7981 agtagtgata tagatgatgt tattttaaat tcattaggtg gcataattgg aattaatatt
     8041 tacataaaca tgaataaatt atttcccaat gatattaaca gatttaaggc tataataggt
     8101 actagtttag ttttaggagt tataggatta ggagttattt ctaaaaatta tcataactta
     8161 ctaactttta aatttagacc tgatagaaaa atatcaaaaa tattaattga agaaaatagg
     8221 gaaataatta aagatataaa taaagataca gtagatatag ttgggacatt tgagagcttt
     8281 aaaaaaggaa taataactat taaagcaggt tctaataaca aagtaaagag accagagaat
     8341 ttaattgatt ctgatggaaa tataagaatt tatttaaatg aaaatacaaa attagtatct
     8401 tatataatta ccaaagaatc aaaaatggat atagttaaat atgaggaatt tgatgtgaag
     8461 aatttagatt tattaaggag atatgatact attaatgttt ggattgataa agaaaatcag
     8521 ccaaaggata aacatggtat tatggctagt aaattattaa ttggattgta tgaataaaat
     8581 attaaacaag aataaaagaa taacaaatat aaaaaacgaa aaaactattt ctataattat
     8641 ttttacgttg tgcataaaat aagaacatta gtgaaggagt agagataaat tatggagata
     8701 ataaaatata tcttgtactt ttttatatat tcatttttag gatggacagt agaaagtata
     8761 ggatgttcta tagcaagcaa aagaataatc aacagaggtt ttttaaatgg tccaatatgt
     8821 cctgtttatg gttttggagc ggtaattgtg atatcactat tgggaagatt taataatgta
     8881 gtaatagtat ttctattagg aatgatttta actacgatac tagaatattt tacaggattt
     8941 attttagaaa ccttatttca tgctaaatgg tgggattatt ctgacagaaa attcaatata
     9001 aagggtagag tatgtttaaa aaatgcaata tactttggtg taatgtctgt tttaataata
     9061 agatttatac atccatttat aaaatatttt gtaagtataa ttccatatag aatattaatt
     9121 tcaatggcta taattacaac actttggact atacttgatt tgattgttac tattataaca
     9181 cttaaaaaat tagatataaa gttaaactta ctagacgata taattacaga ccttaatgat
     9241 ataaatgtga aattggataa atttgataga ggagaaatac aagctttatt taaaacaata
     9301 aataacgacg aattagaagt tagagaaaaa ataaacaaaa taaatagtaa gttggaccat
     9361 atagagtcta atattatttt acaaaaaaga gttataaaag cttttccaca tataaaacat
     9421 aaaaaacaac aagagcaact agaacatttt aaaaaaatta ttaatgagaa aaggtaatta
     9481 tacaatttaa tgaagacgaa tagaataatg gtatttataa gatgattttc agaggactct
     9541 ataaaaatag aatagagtcc tttatttttt gaaaaataat gtttatttga taaaaataaa
     9601 atatattgtt tctgaataac aaatattttg aaaattataa aaacatatat tgacaatttg
     9661 tgtcaaaaat actaacatta agtatataaa gaattttata tgagaatatc tttataaatc
     9721 gcaataattt taaaacataa atttatttaa agaggggatg atttgccaat gagcgagata
     9781 actagtcaaa tgataagtac agaagaaaaa aaatatgttg ctaaaactca aaagatacct
     9841 tattatccag tagcatttaa atcaggtgat ggtgcaatgc tatatgatta tgaaggtaat
     9901 gaatatgtag attttttagc aagtgcaggc tctgcaaatg taggtcatgg taataaggaa
     9961 atctcacaag cagttaaaga acaaatggat gatataacac aatatacact tgcatatttc
    10021 catagtgatc ctcctgtaaa attagctgag aagcttgtgg aaatagcccc tggagataat
    10081 gataaaaaag tactatacag tgcaactggt tctgcttgta tagatgctgc tataaaatta
    10141 gcaagaggat atactggaag aacaaagata atatctatgt gtgaatctta tcatggaagt
    10201 acttatggag ctatatctat atctgcttta agtacaaata tgagaagaaa aatgggtcca
    10261 ttgttaccag aagtttatca cttccattat cctgataaaa atagaacagc taaagaatgt
    10321 ttagatgaga tagaatatgc ttttgcacac tatctaccag cagaagaggt agctgcaata
    10381 tttatagagc caattgcagg agatgcagga ataatagtgc ctccagtaga gtgggtacaa
    10441 ggtttaagta aaatatgtaa agaaaatgga atattacttg taagtgatga aatacaacaa
    10501 ggtatgggaa gaacaggtaa gtggtttggt atagaaaatt ttggtgttga agcagacctt
    10561 atagttcttg gaaaatctgt tggaggtgga ttacctctag gagcagttgt tggaagaaca
    10621 gaaataatgc aaagtcttga tgcaccagca catttattta ctttagctgg aaacacaaca
    10681 gtatgtgtgg ctgctttaaa atctatagag ataattgaaa aagaaaactt acttcaaaag
    10741 agtatagaaa tgggagatta tattaaagca ggatttgaaa aactaaaaga aaaatacgat
    10801 ataattggag aaataagagg cataggatta tctataggag ttgatatagt taaaggaaaa
    10861 ggttctaatg aaaaacatcc agatgctaca gctaagatat gttatagatg tatacaaact
    10921 ggattaataa tgatattctt aggacaatct actttaagag tgcaaccacc tctagtaata
    10981 actaaggagc aagtagataa agcaatgaac atcatagatt ctgcaataga tgattattta
    11041 aatggtagaa ttggtgatga agtatatgaa gtaactcaag gttggtaaaa taaatattaa
    11101 atgaaaatag gagttatctc aattttagaa aattatttct gaaatgagat aactctttta
    11161 agttacccac aaataagaaa attgtttgat tcattaaaga ctatatttcg gatgtcgttt
    11221 ttgtgagtat cagagacagt attacgagaa taattacctt tattcgtact atctgttaat
    11281 ttagttataa ttaagatagc taaaagaata agaattactg tgtataaagc aataacatat
    11341 ggttttcttt tatatttgta tttgttattg aaaaaagaat ggccataatt atttttattt
    11401 actttagatg tatttgtatt ctttttcttg ttacctttct ttttatcatc cttttttcta
    11461 tttttagggt aaggaatgac tttgtgtgta ttatcaagtt tgatattttt atttttttga
    11521 atattgtttt ccaggctagc ctttttttct ccaaattgaa tagttttatt ggtattgttg
    11581 ttatctttca atgttaatct ccctccctta aactttccct ttagataata ttactactaa
    11641 cctatggaat atacaataaa taatatataa gtaataaaat tttataggta taaatctctt
    11701 tctccacgct ctaaggcagt catattgtta gatactatgt ttcttatatc ttctctttct
    11761 atcttaccta tttcttctgc gataagtttt ttacccataa ttttaagttc ttcatctcca
    11821 taatcgatta aaaattcatt taatgtagta agagcatttg gtgtacatac attatggatt
    11881 tgtcgacttt tagctaagct catgaatcta tcgccagttc tacctttacg ataacaagct
    11941 gtacaataac taggtatata tccatcagta atcagttctt taagcacctc aacagggctt
    12001 ctatggtctc caacttcaaa ttgttctgta ttgtcaccat cttcatatgc tttatatcct
    12061 ccaacacctg ttagagaacc tgcactaatt tgtgatacac catattttag taactcattt
    12121 ctcatttcag cagtttctct agtggacatt atgattccag taaaaggaac agccaatcta
    12181 gttatagcta ctattttttt gaacatatcg tcagaaacta agtgaggaaa atcttctaag
    12241 ctcatacctt cagctttttt caatctaggg aaagatatag tatggaaacc tacaccaaat
    12301 ttttcctcta agtgttcgtt gtgcatcata agtcctagaa cttcaaattt agggtcagat
    12361 aaaccaaata aaactcctgc accaacatca tcaacaccag cttccatagc tctatcaaaa
    12421 gctgttaaat ggtaataata gtcatttttt atagattgac catgcatttt tataaatgtt
    12481 ggtttgtgat aagtttcttg gaataatata taagttccta ttcctttttc ttttagtaat
    12541 ttatactcat ctacacttgt agctgctatg ttaacgttta cccttcttat atttccgttt
    12601 tcattatatg ttgaataaat tgcatctaag caatctaaga tgtaatttat atcacaattt
    12661 ttagggtctt caccagcttc tagagcaagt cttttatgac ccattttttc taatatttta
    12721 acctcttcct ttatctcttc catggtaagt ttttttcttt taaatttgtt gcatttacta
    12781 aaaccgcaat aaacacattc atttacacag tagtttgata cataaagagg ggcaaataaa
    12841 accactctat taccatatat ttcatttttt atttgcccag ctatactaaa taatctatct
    12901 agttgttttt tatcttctac ttcaagaagt gtagctatat ctttataact aagtttttcc
    12961 cttctatcag ccttatcaag taccttttca atatcatcag aagttgaatt ttttgcatct
    13021 tctaataaag aattaataag ttcatggttt atgaacattt acaaacgcct ccattcaata
    13081 ttatcaccgc gagaatagtc aacttcaagt cctaatgact tgatgttttc ttctaaactt
    13141 tggtgatatt cacaagcttc ttgaccagta aaggctttgt tatcgtaaag agaatattgt
    13201 tttctaaatt cttgaggaga aaggtttggc ataattacat tgcaaccagc taaaagacct
    13261 gcatttctac cactagggtt tatagatgcc aaggctgttg tagcgggtaa taaaactttt
    13321 ggaagtagaa gtcttgtaat tgaaagcata agtaatgtct tctctaaatc tccatgttta
    13381 taatccttaa aaattgtatc atggtgtgga ataaaaggtc ctattccaac catatgagga
    13441 tttaactctt ttaggtataa taaatcacga acaaggtctt cgttgttctg aaatggtgat
    13501 tcaaccataa atccagcacc tacctgataa ccaatttctt taaggttttt taggcattct
    13561 tttctagttc taagctcaat gtttggcgga tgtaattttt tataatgact gtctgtagca
    13621 gtttcatgtc ttaaaagata tctatctgca ccacataaaa agtatttttt atacgaatca
    13681 taactctttt ctcctagtga tagagttatg gcacaatcct catatttatt ttttatactt
    13741 gaaacaattt cacatatttt atcatcagta aaatatgcat cttcaccacc ttgcaaaacg
    13801 aatgttctgt atcctatttt ataacctata tcacaacatt caagtatttc atcaagtgaa
    13861 agcctgtatc ttatgatatt tttattagaa gattgtattc cacaatagta gcaattattt
    13921 ttacaatagt ttgtaagctc tataagtcct ctcaagtaga ctctatttcc ataatgttta
    13981 tttctcatgt cgctagcttg tttataaaga tagtttctta aattttgttc gaagttatat
    14041 tcctcattgt cgatattttc tattaaatat agtagttcat caaaacttaa tgaattaatg
    14101 ctgtaaagtt tatctataaa gtatttaatc ttatttttag tcatattcaa atatgtaatt
    14161 attaagtata gttaaatttt taataattga aaagttcaag tgatctttcc aaaatacctt
    14221 ttacatatgc tattaagaca ccatagttta caataggtac attgaaagat tttgcctttt
    14281 ctattctaga taacattcca gctctattca tcatacaagc tccacagtga actacaaggg
    14341 catatttatt tatatcttca gtgaaggata ctccagaact aaattcaaaa gtaatgtttt
    14401 tgccagtttt ctttctaatc atattaggaa tttttactgt tccaatatca tcagtttgtc
    14461 tatgatgggt acatccttcg gccatcaaga ttttatcacc atcttttaaa ttttctaaag
    14521 cataagctcc attaatcaat tctttaaggt ctcccttttg tctagcaaac aatatagaaa
    14581 aagatgtaag aggaatatct ttttgtgtat ctttatctac ttgtggaaat acttgagagt
    14641 cagtaataac aagttttggt tttttgccta aattagatag agtttctttt aaactatctt
    14701 cttttgtaac tatagaaatt gcaccattat ccaaaatatc tcttataact tgctgttgag
    14761 gaagtataag tctacctttt ggagctgctt tatctattgg aacaactaaa acaactaaat
    14821 catttggttc aattaaatct gataccaatt tgaactcagt gctatcctta ggcaaaactt
    14881 ttattatttc attttttaaa ttttcaattc caattttatc tgttgatgag acagaaacaa
    14941 ctggacattt aactttattt ttagttaagt ttaaaatttc tgattgattc ttaatagtat
    15001 caattttgtt aagaattaga atatgaggga tattcttatc attaaatttt tcaattagtg
    15061 acaaatcttc ttgtgatata ccaatttggc aatccactac tagtaaagct atatctgtct
    15121 tttctaaaac atctagtgat ttacctattc taagttcacc aagttcacca acatcatcaa
    15181 gaccagcagt atctataaga acacaaggtc ctataggaag gatttccatt ggtctaaata
    15241 ctgggtctgt agtagttcca gcaatatctg agactattgc agcactttga tttgttatag
    15301 catttattat gctagattta cctgcatttc ttttaccaaa gagacctata tgtactctta
    15361 ccgattgtgg agtagaattt agactcattt gtactcacca ccttaaactt ctttttttga
    15421 tatactagtt tttacagata catgaggtat gttgcccaat ttaccagtca aactattaat
    15481 agtgtccatt tcgccaacaa ctgctataca tacgattgag acaccttctt cttcgcaagg
    15541 aatgcccatt cttcctttaa taataccttt aaaacctgat acaacctcat tgaatttaaa
    15601 ttgacattct tttggttctt ccaaaatagc acttataact gcaacctttt tcataaatat
    15661 cctcctaact cagaaaatat gtcttaaaca aatccaaaat caaaaaactt ttctttgtaa
    15721 agaaaagttt ctacaaaaga aaagttatta attacacaag tataatgtgc tatattaaac
    15781 tatttcctta cagattagat aactcttttc tggtagggtt agcttgtaaa aatttaattt
    15841 aacaacaagt aactgaagaa aatttttata caatttatat aaattataat aaaaattaac
    15901 taaagaaaa
//
LOCUS       DAMJYR010000088        15678 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.090, whole genome shotgun sequence.
ACCESSION   DAMJYR010000088 DAMJYR010000000
VERSION     DAMJYR010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15678)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15678)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15678
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.090"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(96..956)
                     /locus_tag="PC471_002650"
     CDS             complement(96..956)
                     /locus_tag="PC471_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-ADP-ribose hydrolase"
                     /protein_id="HDF2789767.1"
                     /translation="MAMKWRDYADDVNLFEDFDKTIKPLTDEQRRKNINTLIAYFSKE
                     VPSKIYNLSNDEIKPRDILRGLLNVYPPKEIAPEILNMLHNLLLIECEERELVDVNDI
                     EEIEEGIAIWRGNITNLRADAIVNAANNKLLGCLQPLHLCVDNEIHSCAGPRLREDCD
                     KIIKKQGHLEYTGDAKITRGYCLPAKFVVHTVGPIVSGGQPSKEQEKQLLHCYKSCLN
                     TIKEIDEIKNIVFCGISTGVFGYPKKEAANLAVSRVRLWLKENPEKNLKVVFNVFTEE
                     EEEKYRRIFK"
     gene            complement(1273..2601)
                     /locus_tag="PC471_002651"
     CDS             complement(1273..2601)
                     /locus_tag="PC471_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010963854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-alpha-glucosidase"
                     /protein_id="HDF2789768.1"
                     /translation="MEKFSVVIAGGGSTYTPEIILMLLDNLDRLPLRAIKLYDNDEER
                     QNKVAKACEILIKEKDPNIEYLATTCPKEAYTDVDFCLAHIRVGKLEMRELDEKIPLK
                     HGVVGQETCGPGGIAYGMRSIEGVLENIDYMEKYSPNCWMLNYSNPASIIAEAVRRLR
                     PNSRVINICDMPIGMEHNIARIAGLKSRKHMDIRYFGLNHFGWYTSIKDKKGNELLPK
                     LVEHIKEYGFINGEEGMKNDKKDSWFETNLFTKEIVKTDLTTIPSSYLKYYLFPDYVV
                     NHSDVNYTRANEVIDGREKEVFGSCALIEKQGHSKGSGLKIGIHAEFIVDLATALAFN
                     TQERMLLIVQNNGAIENIQDDAMVEIPCIVGKDGYEPLSIGKIPTFQKGLIEQQLAVE
                     KLVVDAWIEKSYNKLWQALMLSKTVPSATVAKELLDDLIEANKNYWPKLN"
     gene            complement(2749..3078)
                     /locus_tag="PC471_002652"
     CDS             complement(2749..3078)
                     /locus_tag="PC471_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HDF2789769.1"
                     /translation="MVPEMEEKVFSIISEAGDAKSDVMMSLKEIKKGDYNKAKHLLNS
                     ASEKIQTASKHHLELLSNAMNSEDSGTDFLVVHSEDHYSNALFAHSLVSELVDIFEMM
                     DPRINKN"
     gene            complement(3102..4391)
                     /locus_tag="PC471_002653"
     CDS             complement(3102..4391)
                     /locus_tag="PC471_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDF2789770.1"
                     /translation="MEALQNNLRRFLLPIAQKIEKQRHLQAIKEGMISITPIIIVGSL
                     SMLFMALNNMLPEGSAKTLLSENMDTLLIPNKFTMSLLSIYSAFFIAQALAKKYNLNH
                     VEIGMTAVVAQLVVCGQVVDGVLDTSYLDAQGLFVSILVALLVVDITKFMNDKNLVIR
                     FPKEVPSVVNKSFRNLTPMIVCIMLFTAIAAITKNVSGQPLPAIIMNFLAPAISSVDN
                     VFAVTIILFITQLLWFFGLHGAAITSSIWMPIAATYMAENATLIAAGGDPKYVFTIGF
                     YYGFLQVTGSGITLGLVYLMSRSKCKSFNSMGKVVILPSLFGINEPVIFGTPIVMNPY
                     MFVPFVFGPVLVGALNFMALKVGLVGLPIAEPPGFLPPGVAAFLMTLDWKAIVLVFAS
                     IILMTLIYYPFFKIMEKEELNKNAELATTLDDDSFDF"
     gene            complement(4407..4733)
                     /locus_tag="PC471_002654"
     CDS             complement(4407..4733)
                     /locus_tag="PC471_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDF2789771.1"
                     /translation="MNILLVCANGASTGVLVEKMKSFCSEHEKLKTKTINIEATSFEN
                     LKSYIEANDTDVVLVAPQIRFKEDDVVEACKNYKIAVGLIDTKHYGRMDAPSVMKSAI
                     ELYKNR"
     gene            complement(4868..5497)
                     /locus_tag="PC471_002655"
     CDS             complement(4868..5497)
                     /locus_tag="PC471_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2789772.1"
                     /translation="MQDKILRDKKANDPWARMTTRNGFSADEIISSFQKAIRRNMVEE
                     ACEFAYELYISSPELEDKLWRRILTISVEDIGMGDPSAVIIINNLNQVRKEYSYADGD
                     RPLFFIHAIRYLCACEKDRSSDLLKNIIIKGFAMGKVPEVMDVALDKHTYRGLEMGRD
                     SFHFLNEASIVIPEKEVDNDYKERYLKILEKYKQEEVIDTAFKFNHWQY"
     gene            complement(5712..8369)
                     /locus_tag="PC471_002656"
     CDS             complement(5712..8369)
                     /locus_tag="PC471_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDF2789773.1"
                     /translation="MIYKKDKVMKVLESSYRENEKGITANQLSEKTGIARNNISTYLN
                     QLFNEGMVTKIKGRPVYYIPANLNLNKNELLDTQSELLGIKKGTKVENKIKKDSFETL
                     IGKEHSLRPIIEKCKVAMLYPTNGLHTIIYGETGVGKSMIARYMYDYSIDSGIRKNKA
                     PFVTFNCADYANNAQLLMGHIFGVEQGAYTGAESSRKGLLEIANGGILFLDEIHRLPA
                     EGQEMLFTFIDKGTFKRLGDATKERESKVLIICATTENPTSTLLDTFNRRIPMKIEIP
                     SLRDRTMIERMELVKNFFKEESKSISISIKVHKEIIKSLLLYDCKNNVGQLQNDIKLA
                     VANGYLRYKRDNKEPVYIEKKYFDKSINEVSDDYREKSIKADEVITHGIEYFVFTSLG
                     EEEVINYNDINIINAVNTNLNKSVEMNIADASLFNNVKFKSMCESIRQIIKEDEEIDI
                     SESIFKSMAIYIKSVLDKSSSTNNIDLNQIRRNNRSEFKTALKIVGIIENEFDLFLSI
                     ETVAYITLFIVQAKQDEQNKIVNNINIIVAMHGETTASSMVKAVGDIIGKCSAISFDM
                     KLDKSYDEVIVDFKNLINNIDNKDVLLFTDMGSLNSFDEIIKKEKKCGVRVIPMVTTL
                     TVLEAVQKANMGLPLNDVYNSITNTRKYYFGTNEIQNKENLSKTIIIASHVSEGVDNK
                     TRKILEEKMSRYLDGIDIISVPYKTEKDLSLNITKLKESSNIVAVINEQRINIRGIDY
                     ISKKDIDKDENINKLKNIIKISIGYDDVVEGLKTSLKSSNYNRIFKDIKYVSDELFLV
                     FNIEKKYDKVIGLMMHLAFMVDGLIGNTREIEKLDKEKTLDYHKSLSKIKDIVSQLDK
                     KYNIEINEKECYQILLILEYAEIIEKDYQ"
     gene            complement(8728..9672)
                     /locus_tag="PC471_002657"
     CDS             complement(8728..9672)
                     /locus_tag="PC471_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009524518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2789774.1"
                     /translation="MNREKILEIKNLKQYFHLDKSTTVKAVDDISFDIYKGEIFGLVG
                     ESGSGKSTTGKTIIRLHESTGGEVIYKGNCISDKKTYKFIKKDVNKSMQIIFQDSTSS
                     LNPRMTIADIISEPLKIQGICKNKTDRMNKVYEMLKLVGLDRSYANKYPSDFSGGQRQ
                     RIGIARALSVDPEFIIADEPIASLDVSIQAQIVNLFKKLQQEKNLTCLFIAHDLSMVR
                     HISDRIGVMYNGKLVELADSNELYNNPIHPYTKSLLSAIPVPDPRYAKSRNRIEYNSN
                     GYDCSNEKSLSWIDVSDGHFVYSSKSEINKYQQNLKVV"
     gene            complement(9665..10690)
                     /locus_tag="PC471_002658"
     CDS             complement(9665..10690)
                     /locus_tag="PC471_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2789775.1"
                     /translation="MEHLLEVNNLSVSFKVEEGEVQAVRNVSFNLKKGETLAIVGESG
                     CGKSVLCKSLMRILPYNGYIKNGEVLLKSSDLVKKSEKEMEDIRGKNISMIFQDPMTS
                     LNPTISIGKQIAEAVIIHQGISKSEAKKRAIELIELVGIDNPEKRFKQFPHHFSGGMR
                     QRIVIAIALACNPDVLIADEPTTALDVTIQAQIIDLIKDLQHKIGLSIIFITHDLGVV
                     ATIADRIAVMYAGKIVEIGTVEDIFYDPRHPYTWGLLGSLPTLDSQDDYLYNIPGMPP
                     NLLNPPKGDAFAIRNKNALKIDYEKEPPMFKINDTHSAATWLLHPDAPEVDVPVRVNC
                     GRVISNE"
     gene            complement(10765..12333)
                     /locus_tag="PC471_002659"
     CDS             complement(10765..12333)
                     /locus_tag="PC471_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDF2789776.1"
                     /translation="MKLKKLKVLSLVMILSLMAGCSSGGDKDKKADTPKDGKVLVYGS
                     NDYTSINPALYEHGEINSLIFNGLTAHDENNKVVPCLAKDWKFDEATNTYTFNLRDDV
                     KWHDGEKFTANDVKFTIETIMNPDNASEIASNYEDITKIDVVNDNTIKITLKAPNTAM
                     LDYLTVGVLPKHALEGKDIATDEFNQKPIGTGPFKLEKWDKGQSITLVKNSDYFVKEP
                     GLDKVVFKIVPDDKAKAMQLKSGELDLAQITPKDMSNFEKDEKNFKVNIMKTADYRGI
                     LYNFNSKFFKDKKAKGLPNALSYAIDRKAIVDSVLLGHGVPAYSPLQMGPYNNPDIEK
                     FEYNPEKAKQEIEKLGWKLGSDGIYEKEGTKLAFEITAGESDQVRVDMAKICAQQLKE
                     IGVDAKAVVVTETDWANQDAHLIGWGSPFDPDDHTYKVFGTDKGANYSAYSNPTIDKI
                     LQKARETEDKDEKLKLYKQFQVEMTKDMPYTFIAYIDAIYVGKPNIKGLTPDTVLGHH
                     GVGIFWNIADWTIE"
     gene            complement(12446..13357)
                     /locus_tag="PC471_002660"
     CDS             complement(12446..13357)
                     /locus_tag="PC471_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2789777.1"
                     /translation="MKPQYSDFEIVGENYILVAEDEHIETKKTLSKKIKELPYISIII
                     LSIIVIGSVFSSFIMTHEPTYMDLASSNLAPNKTFLFGTDSMGRDIYSMIWYGGKISL
                     FIGLFSTVISTVIGIVYGSISGSASELVDDAMMRFTEIILSIPSILIIIFVQAILGNS
                     NPVSMSIVIGITSWMNISKIVRTEVRQIKNSEYILAAKSMGGGFFYVLKQHLLPNFVA
                     SIMFMVVTNIGAAIGTESTLSFLGIGLPIEIVSWGSMLSLSEEALLSNRWWIILIPGI
                     FLVTTLVCITNIGNYIRKNNNKKSSNL"
     gene            complement(13341..14327)
                     /locus_tag="PC471_002661"
     CDS             complement(13341..14327)
                     /locus_tag="PC471_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2789778.1"
                     /translation="MLKDILKKLLQFILVMFLLSFIVFYMARLAPGDPLISYYGDGVE
                     RMSTQEKENAMKKLGLDEPIYSQYIKWISNASKGEFGISFKYKQNVTSVINDVYINTI
                     ILGGSGYILTFVLALLLGIFCTLHEDKLIDRIICKLGTITNCIPSFWVALVLILIFSI
                     NLSILPSSGAYSMGEESSILSRISHLILPLTVLILSHLWYYTYMIRNKLLEEIREDYV
                     LLCKAKGLNNRTIVFKHCLRNIMPSYISIMAISIPHILGGTYVVEKVFSYPGLGTLCF
                     ESAKYHDYNMLLVLCLITGALVVFGNMLAQIINNKIDPRMKYDRGDTNEATI"
     gene            14687..15088
                     /locus_tag="PC471_002662"
     CDS             14687..15088
                     /locus_tag="PC471_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HDF2789779.1"
                     /translation="MLLKELYSKTMYTVEENFKENGLTHQQIIVIKLIAHNQELTISQ
                     LCDEMSLAKGTVSGIISRLEQIGYIEKFKKSNDKRNTYVKFTTTGFEFATNFKIKMQE
                     SFDDIFKNCDENELSDLVKNLRNILAKVKER"
     gene            15091..15489
                     /locus_tag="PC471_002663"
     CDS             15091..15489
                     /locus_tag="PC471_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03987 family protein"
                     /protein_id="HDF2789780.1"
                     /translation="MNYKLILAIVFITSALIFYTIGVFGERKAKILKKKHVIIFWLGF
                     IFDTLGTFTMSNIANSHTFEVKSALSQNLHSITGLLAIVLMLFHASWATFVLYKDDEE
                     KKKFFHKFSIVVWTIWLVPYFIGMFIGMAG"
BASE COUNT         4942 a         2731 c         1730 g         6275 t
ORIGIN      
        1 ttatatttta ttaattttta ctacttgtaa attattatct tatacaatga atttgtatta
       61 tataataagt atttttagga ttctgttaca taatattatt taaatattct tctatatttc
      121 tcttcttctt cttcagtaaa cacattaaat acaactttta aatttttttc aggattttct
      181 tttagccata gtctaactct acttacagca agattagctg cctctttttt tggataacca
      241 aatacacctg tagaaattcc acaaaaaaca atatttttta tttcatcaat ttcttttatt
      301 gtatttaaac atgatttata acaatgaagc agttgctttt cttgctcttt acttggctga
      361 ccaccagata caataggtcc aacagtatgt actacaaatt ttgcaggtaa acaataacct
      421 ctagttatct tagcatctcc agtatattct aaatgccctt gtttttttat tattttgtca
      481 caatcttctc taagtctagg acctgcgcat gaatgtatct cattatccac gcatagatgt
      541 aatggttgta aacaccctaa taacttgtta tttgcagcat ttactattgc atctgctctt
      601 aaattcgtta tattacctct ccatatggca atcccttcct ctatttcttc tatatcattt
      661 acatctacta actctctttc ttcacattca atcaacaata aattatgtaa catattaagt
      721 atctctggag caatttcttt tggtggatat acattcaaaa gtcctctaag tatatctcta
      781 ggcttaattt catcatttga taaattgtat attttagaag gaacttcttt tgaaaaatat
      841 gcaattaaag tgtttatatt ttttcttctt tgttcgtcag ttagaggctt gatagtttta
      901 tcaaaatcct caaataaatt tacatcatca gcataatctc tccacttcat agccatctct
      961 ccattcttgt attttagact taaaaataat tatgtattta tgtacatatt tatttatttc
     1021 tataaacacc tacataaatc ctgttaaaag tgtaaatttt ttattatgca acttctctgg
     1081 ttatttctat tgattgatag taaattattg tacctaaatt taatccaact aacaagtaac
     1141 taaacctact ttcctaactg caaaatacac tatgtttgcc acttgtatct catttgtaaa
     1201 taaataaagt ccctacaaat acattaacta ggctatgtac taaactgtag gaactttaaa
     1261 aatatattat tcttaattaa gttttggcca ataattttta tttgcttcta ttaaatcatc
     1321 tagtaattct tttgcaactg ttgcactagg aactgtctta gatagcataa gtgcttgcca
     1381 caatttatta taagacttct caatccaagc atcaactact aatttttcaa ctgctaattg
     1441 ttgttctatt aaaccttttt ggaatgttgg tattttccct attgataatg gttcataccc
     1501 gtcttttcca actatacatg gaatttcaac catagcatca tcttgtatat tttctatagc
     1561 tccattattt tgaactatca ataacatacg ctcctgtgta ttaaaagcta gagctgtagc
     1621 taaatccact ataaactctg catgtattcc tatcttaagc ccactgccct tagaatgtcc
     1681 ttgcttttct atcaatgcac atgaaccaaa aacttctttt tctcttccat caataacttc
     1741 attggcacga gtataattta catcagaatg atttacaaca taatctggaa atagatagta
     1801 ctttaagtaa ctacttggta ttgtagttaa atctgttttt actatttctt tagtaaataa
     1861 attagtttca aaccaactat ctttcttgtc atttttcatt ccttcttcac catttataaa
     1921 accatattct tttatatgtt ctactaattt tggtaaaagt tcatttcctt ttttatcttt
     1981 aattgaagta taccaaccaa agtggtttaa tccaaagtat cttatatcca tatgtttacg
     2041 agatttgagt cctgcaatcc ttgctatatt atgttccata cctattggca tatcacaaat
     2101 atttattacc ctagaattag gtctcaacct tctaacagct tccgctatta ttgatgctgg
     2161 attagaataa tttaacatcc aacaatttgg agaatacttt tccatataat ctatgttttc
     2221 aagaacacct tctatagacc tcattccata agctattcca cctggtccac aagtttcttg
     2281 accaactacc ccatgtttta gaggtatctt ttcatctagt tctctcattt caagttttcc
     2341 aactcttata tgagctaaac aaaaatctac atctgtataa gcctctttag ggcatgttgt
     2401 tgctaaatac tctatattag ggtccttttc ctttattagt atttcacaag ctttagctac
     2461 cttattttgt ctttcttcat cattatcata taatttgatt gctcttaatg gtaacctatc
     2521 taagttatct agcaacatta atatgatttc tggggtatat gtacttcctc ctccagctat
     2581 tactactgaa aatttttcca taataatatc tccttttcta tttaaaaatt tactgtattt
     2641 ataaataaat ttataaatac tagtacttta aagtaaaagg gagttcattt attaaatcta
     2701 ttgatttaat aaactatagc caaacttcaa gcactattta caagaaattt aattcttatt
     2761 aattcttggg tccatcatct caaatatatc tacaagttca gatactaaag aatgagcaaa
     2821 taatgcattt gaataatggt cttcagagtg cacaactaaa aaatcagtcc cactatcttc
     2881 actattcata gcatttgata gtaattctaa atgatgcttt gatgctgttt gtattttctc
     2941 tgaggctgaa tttaaaaggt gttttgcttt attgtagtca ccttttttta tttccttaag
     3001 agacatcatt acatcacttt tagcatctcc tgcttctgat attatactaa atactttttc
     3061 ttccatttca ggtaccatat ccttctcctt ctaatgactt gttaaaaatc aaagctatca
     3121 tcatctaatg ttgtagctaa ttctgcattt ttatttaatt cttccttctc cattatctta
     3181 aagaatggat aatatatcaa ggtcataagt attatacttg caaatactaa tacgattgcc
     3241 ttccaatcta atgtcatcaa aaatgctgca acccctggag gtaaaaagcc tggtggttct
     3301 gcaataggta atcctactaa tcctactttt aatgccataa aattaagtgc tccaaccaat
     3361 acaggtccaa atacaaatgg tacaaacata tatgggttca taactattgg tgttccaaat
     3421 attaccggct catttattcc aaatagtgat ggtaaaatca caacttttcc catactatta
     3481 aaactcttac acttacttct tgacataagg tacactaatc ccaaagttat ccctgaacca
     3541 gttacttgca agaaaccata gtaaaaacct attgtaaaca catattttgg gtctcctcct
     3601 gctgctatta acgttgcatt ttctgccata tatgtagctg ctattggcat ccatatactt
     3661 gaagttattg ctgctccatg aagcccaaag aaccataata attgagttat aaatagtatt
     3721 atagttactg caaaaacatt atctacactg cttatagctg gtgctaagaa attcattata
     3781 atagctggta atggttgacc tgagacattt ttagttattg ctgctattgc tgtaaataac
     3841 attatacata ctatcattgg agttaagttt ctaaaacttt tatttactac actaggtact
     3901 tcttttggaa atctaattac gagattttta tcattcataa acttagtaat atcaactact
     3961 aaaagtgcta ctaatatact tacaaataaa ccttgtgcat ctaaataact tgtatctaat
     4021 acaccatcta ctacttgtcc acatacaaca agttgtgcaa caactgctgt catacctatt
     4081 tctacatgat taagattata ttttttagct agtgcctgag ctataaaaaa tgctgaatat
     4141 atagataata aactcatagt aaatttatta gggataagta atgtatccat attttcagat
     4201 aataatgttt ttgcacttcc ttcaggtaac atattgttta atgccataaa taacatactt
     4261 aaagaaccaa caattattat tggggtaatg cttatcatac cttcttttat agcttgaaga
     4321 tgcctttgtt tttcaatctt ttgtgctatt ggaagaagaa atcttcttaa attattttga
     4381 agtgcttcca tttttattcc ccccaattat ctatttttat ataattcgat tgccgacttc
     4441 ataacagaag gagcatccat acgaccataa tgcttagtat ctattaaacc aactgctatt
     4501 ttgtaattct tacaagcttc aactacatca tcttccttaa acctaatttg tggtgctaca
     4561 agtactacat cggtatcatt agcttcaatg tatgatttta aattttcaaa tgatgttgcc
     4621 tctatattta tagttttagt ttttaacttt tcatgctcac tacaaaaact tttcatcttt
     4681 tccacaagta cacctgttga agctccattt gcacatacta ataaaatatt cataattacc
     4741 ctcctacaat atctaacctc ttttattttc taatttttta tcagccaatt cttactctaa
     4801 ttcctatcag tttttacttt aattcttatt attttatttt aattcttatt aatatttact
     4861 ctaattctta gtactgccaa tgattaaact tgaaagccgt atctataact tcttcttgct
     4921 tatatttttc taatattttt aagtatcttt ctttataatc attatctact tctttttctg
     4981 gtattactat actagcttca tttaaaaagt ggaatgaatc ccttcccatt tctaatcctc
     5041 tatatgtatg cttgtcaagt gccacgtcca taacttctgg aactttaccc attgcaaatc
     5101 cttttataat tatatttttt aataaatcac ttgatctatc tttctcacat gcacaaagat
     5161 atcttattgc atgtatgaaa aataaaggtc tgtctccatc tgcatatgaa tattcctttc
     5221 ttacttgatt taaattattt atgattatta cagctgatgg gtcccccatt cctatatctt
     5281 caactgatat tgtaagtatc cttctccata gcttatcttc taattctggt gatgatatat
     5341 atagttcata agcaaattca caagcttctt ccaccatatt tcttcttata gctttttgaa
     5401 atgacgatat tatttcatct gcactaaatc cgtttcttgt agtcattctt gcccatgggt
     5461 catttgcctt tttatctctt aatattttat cttgcatatt atttactcct ttttatttaa
     5521 attttaatta tttatattac tcatttaata atataagcaa gtattatgcc agttttttta
     5581 gacactttaa aacactgtta tttcaatcat ttttatcatc aaaacttaga tactttgtct
     5641 aagtatattt atatgtgtct aatattaata ttattttaga ctaaaaaaaa ttaccaaata
     5701 agtttttata ctcattggta atctttttcg attatttctg catattctaa tattaataat
     5761 atttgatagc actctttttc attaatttct atattatatt ttttatctaa ttgagatact
     5821 atatctttta ttttgcttag gctcttgtga taatcaagtg ttttttcttt atcaagcttt
     5881 tcaatttctc tagtatttcc aatcaatcca tctaccatga aagctaaatg catcataagt
     5941 ccaattactt tatcgtattt cttttctata ttaaatacta aaaacaattc atcagacaca
     6001 tattttatat ctttaaatat cctattataa ttagagcttt ttaaggatgt ctttaaacct
     6061 tcaactacat catcataacc tatacttatc tttataatat tttttaattt attgatattt
     6121 tcatccttat ctatatcttt tttgctaata tagtcaattc ctctaatatt aattctttgt
     6181 tcattgatta ctgcaacaat attactactt tctttaagtt tcgtaatatt taaacttaaa
     6241 tctttttctg ttttatatgg gacagatata atatctattc catctaagta cctactcatc
     6301 ttttcttcta atatttttct tgttttatta tctacacctt ctgaaacatg tgatgctata
     6361 ataattgttt tagacagatt ttctttattt tgaatttcat tagttccaaa atagtacttt
     6421 ctcgtgttag ttatagagtt gtatacgtca tttaaaggta atcccatatt ggccttctga
     6481 actgcttcta acacagttaa tgttgtcacc ataggaatta ctcttactcc acactttttt
     6541 tctttcttta taatttcatc aaaagagttt aatgaaccca tatctgtaaa tagtaataca
     6601 tctttattgt ctatattatt aattaaattt ttaaaatcta caataacttc atcatagctt
     6661 ttatctaact tcatatcgaa agaaatagca ctgcactttc caataatatc accaactgct
     6721 tttaccatac tacttgctgt agtttctcca tgcatagcaa ctataatatt aatgttattc
     6781 actattttat tttgttcatc ttgtttagct tgtactatga ataatgtaat atatgctaca
     6841 gtttctatag ataaaaataa atcaaattca ttttcaatta ttccaactat ttttagtgct
     6901 gttttaaatt cacttctgtt atttcttcta atttgattca aatctatgtt gtttgtagag
     6961 ctacttttat ccaatacgct ttttatataa attgccattg acttaaatat tgattcactt
     7021 atgtctattt cttcatcttc tttaataatt tgtcttattg attcacacat gcttttaaac
     7081 ttaacattat taaatagaga agcatctgct atattcattt ctacagattt attaagatta
     7141 gtatttactg cattaataat atttatatca ttgtagttaa taacttcttc ttctcctaat
     7201 gacgtaaata caaaatattc tattccatgt gttataactt catctgcttt aatagacttc
     7261 tctctataat catcactcac ttcatttata gatttatcaa aatatttttt ctctatataa
     7321 actggttctt tattatctct tttatatctt aggtatccat ttgcaacagc taattttata
     7381 tcattttgaa gttgacctac attattttta caatcataaa gtaatagtga tttaattatt
     7441 tctttatgta cttttattga aatagaaata ctttttgatt cttccttgaa aaaatttttg
     7501 actaattcca ttctttcaat cattgtccta tctctcagtg atggtatttc tattttcatt
     7561 ggtattcttc tattgaatgt atctaatagt gttgatgtcg gattttctgt agtagcacat
     7621 attattaata ccttagactc tctttctttc gttgcatctc ctagtctttt aaatgttccc
     7681 ttatctataa aagtaaacaa catttcttga ccttctgctg gtagtctatg aatttcatct
     7741 aaaaatagta tccctccatt tgcaatttcc aaaagacctt ttctggaaga ttctgcacct
     7801 gtataagctc cttgttctac cccaaaaata tgtcccataa gtaattgtgc attattagca
     7861 taatcagcac aattaaatgt tacaaaagga gctttatttt ttctaattcc actatctata
     7921 gaataatcgt acatatatct agcaatcata ctctttccta ctcctgtttc tccatagata
     7981 atagtatgaa gtccatttgt aggatagagc attgcgacct tgcatttttc tattataggt
     8041 cttaaactat gttctttacc tattagtgtt tcaaaactat ctttttttat tttgttttct
     8101 acttttgttc ctttttttat gccaagcaac tcactttgtg tatcaagcaa ttcattcttg
     8161 ttaaggttaa gatttgcagg gatataatac acaggtcttc cttttatctt cgtaaccata
     8221 ccctcattga ataattggtt taaatatgta cttatattgt ttcttgcaat tcctgtcttt
     8281 tctgataatt ggttagctgt aatccctttt tcattttccc tatatgatga ttctaaaact
     8341 ttcattactt tatctttttt atatatcatt acaagctccc ctacattata gtattttatt
     8401 aactaaaacc cctatacaca ctttattttt taataaactc ttattcttta actaaggtta
     8461 taatttatca aactttcctt actttaaatt atagaaaatt attgtagaaa aaataagatt
     8521 attgctcatt ttttctacat tactattttt aattttatag tactttctta acaacatact
     8581 ttacttgttt ttaattttat tatatttgaa tttattatat ttgaaaagta gaaataaaaa
     8641 accctatttt ggagtagttt taaaatttct ccaaaaatag ggttttagtt tgtatttcaa
     8701 ataaatacct gttcaagtat ttttatacta aacaactttt aaattttgtt gatatttatt
     8761 tatttctgat ttagaagaat atacaaaatg tccatctgaa acatcaatcc aacttaatga
     8821 cttttcattt gaacaatcat atccattaga attatactct attctatttc ttgacttagc
     8881 atatctaggg tcaggaacag gaattgcaga aagtagtgat tttgtatatg gatgaatagg
     8941 attattatac aattcatttg aatctgccaa ttctactaat tttccattat acataactcc
     9001 tatacggtca ctaatatgtc tcaccataga taaatcatga gctataaata aacaagttaa
     9061 attcttttct tgttgcaatt ttttaaataa atttactatt tgtgcttgta tagacacatc
     9121 caaagaagct ataggctcat cagctattat aaattctggg tctactgaaa gagctcgtgc
     9181 tatgccaata cgctgtcttt gtccacctga aaaatcagat ggatacttat ttgcataact
     9241 tctatcaagt cctactagct ttagcatctc atatacttta ttcattctat ctgttttatt
     9301 tttacagata ccttgaattt ttagtggctc tgaaattata tctgcaattg tcatacgtgg
     9361 atttaatgat gaagtagaat cttgaaaaat aatctgcata cttttgttta catctttttt
     9421 tataaatttg tacgtttttt tatctgatat gcaatttcct ttgtaaatta cttctccacc
     9481 agtagattca tgtagtctta ttatagtttt accagttgta gattttcctg aacccgactc
     9541 tcctaccaat ccaaatatct cacccttata tatatcaaaa cttatatcat caactgcttt
     9601 tacagtagta cttttgtcca aatgaaaata ttgctttaag ttttttattt ccagtatttt
     9661 ttctctattc attagaaatc acccttccac aatttactct aactggtaca tctacttcag
     9721 gtgcatctgg gtgcaataac caagttgctg cactatgtgt atcattaatt ttaaacattg
     9781 gtggctcttt ttcataatct atcttaagtg catttttatt ccttattgca aaagcatctc
     9841 cttttggtgg gttcaataaa tttggaggca ttccaggtat attatataaa tagtcatcct
     9901 gcgaatctaa agtaggtaat gaacccaaca acccccaagt atatggatgt ctagggtcat
     9961 aaaatatatc ctctacagtt cctatttcta caatctttcc tgcatacata actgctattc
    10021 tatcagctat agttgcaact actcctaaat catgagttat aaatattatt gacaaaccta
    10081 ttttatgttg taaatccttt attaaatcaa ttatttgagc ttgtatagtt acatctaaag
    10141 cagttgtagg ttcatcagca atcaatacat ctggattaca agcaagtgct atagctatta
    10201 ctatacgttg tctcattcca cctgaaaaat gatgtggaaa ttgtttaaat cttttctcag
    10261 gattatcaat tccaaccaat tcaataagct ctatggctct cttttttgcc tcacttttac
    10321 ttataccttg atgaatgatt acagcttctg caatttgctt tcctattgat atagttggat
    10381 tgagagatgt catagggtct tgaaatatca ttgatatatt ctttcctctt atatcttcca
    10441 tctctttttc agacttctta accaaatcac ttgatttaag taaaacctct ccatttttaa
    10501 tatatccatt gtatgggagt atcctcatca aactcttaca cagtacagat ttcccacaac
    10561 cagattctcc aactatagcc aatgtctcac ctttttttag attaaaagat acatttctta
    10621 cagcctgaac ttctccttct tctactttaa aacttacaga tagattatta acctccaata
    10681 aatgttccaa aaaccatcaa cccctatgct attcaaacct tagggatata tttttatccc
    10741 taaggtttct aattaatttc agtattattc aattgtccag tcagctatgt tccagaatat
    10801 acctacacca tgatgaccta aaactgtatc aggtgttaat ccttttatgt ttggttttcc
    10861 aacatatatt gcatctatat aagcaataaa tgtataaggc atatccttag tcatttctac
    10921 ttggaattgt ttatataact ttaatttttc atctttatct tctgtttctc ttgctttttg
    10981 aagtatttta tctatagtag gattagagta agcactgtaa tttgcacctt tatctgttcc
    11041 aaatacttta tatgtatggt catctgggtc aaatggactt ccccaaccta ttaaatgagc
    11101 atcttgatta gcccaatctg tttcagttac aacaacagct ttagcatcaa caccaatttc
    11161 ttttaactgt tgtgcacata ttttagccat atcaactcta acttggtcac tctcacctgc
    11221 tgttatctcg aaagctaact tagtaccttc tttttcatag attccatcag aacctaattt
    11281 ccaacctagc ttttcaattt cttgtttagc tttttctgga ttatattcaa acttttctat
    11341 atcaggatta ttatatggtc ccatttgtaa aggtgaatat gctggtacac catgtcctaa
    11401 taatacactg tcaactattg cttttctatc tattgcatag cttaaagcat ttggtaatcc
    11461 tttagctttt ttatctttaa agaatttaga attaaaattg tataatatac ctctatagtc
    11521 ggctgttttc attatattta ctttaaaatt cttttcgtct ttttcaaagt tagacatatc
    11581 tttaggagtt atttgagcta ggtctaattc tcctgatttt aattgcattg ccttagcctt
    11641 atcatctgga acaatcttaa agactacttt atctaatcct ggttctttta caaaataatc
    11701 actattctta acaagtgtta tactttgacc tttgtcccat ttttcaagtt taaatggtcc
    11761 agtacctatt ggtttttgat taaattcatc agtagctata tccttacctt ccaaagcatg
    11821 ttttggtaat actcctactg ttaaataatc aagcattgct gtatttggag cttttaaagt
    11881 aatttttata gtattatcat taaccacatc aatttttgtt atatcttcat aatttgatgc
    11941 tatctcagaa gcattatctg gattcattat agtttctatt gtaaacttaa catcatttgc
    12001 tgtaaatttt tctccatcat gccacttaac atcatctctt aaattaaaag tatatgtatt
    12061 tgttgcctca tcaaatttcc agtcctttgc tagacaagga accaccttat tattttcatc
    12121 atgagctgtt aatccattaa atattaacga atttatttct ccatgttcat atagtgcagg
    12181 atttatactt gtatagtcat tacttccata aacaagcacc tttccatctt taggcgtatc
    12241 tgccttctta tctttgtctc ctccactaga acaacctgcc attagactaa gtatcattac
    12301 taaacttaaa acttttaact tctttaattt catgatgctc ccccttttgc tttttataaa
    12361 atatttttca aagctttgac cccatttatt taaatagata tttcaaatac ctcagtattt
    12421 gcttatttaa tactcaaatg ctgatttata aattactaga ttttttatta ttattctttc
    12481 ttatataatt acctatattt gttatacaca ccaatgtggt aaccaaaaat atacccggaa
    12541 tcagtataat ccaccatcta tttgaaagta atgcttcctc tgacaaagat aacatacttc
    12601 cccaagaaac aatttctatt ggtaaaccaa ttcctaaaaa gctaagtgtc gattcagtac
    12661 caatagctgc gcctatgttt gttacaacca tgaacattat tgatgccaca aaatttggaa
    12721 gtaaatgttg ctttaataca tagaagaatc ctcctcccat gctcttagct gctagtatgt
    12781 attcgctatt ttttatttgt ctaacttctg ttctcactat cttagatata ttcatccaac
    12841 ttgttatccc tattactatg gacatagata ctggatttga atttccaagt atagcttgta
    12901 caaatattat aattaatatt gaaggtatac taagtatgat ttctgtaaac ctcatcattg
    12961 catcatctac aagttcagat gctgaaccac ttatactacc ataaacaatt cctataacag
    13021 ttgatattac agtggaaaat agtcctataa ataaagatat ttttcctcca taccatatca
    13081 tagaatatat atctctaccc attgagtctg ttccaaataa aaaagtttta tttggagcta
    13141 aattagaact tgctaaatcc atgtatgttg gttcatgtgt cattataaaa gatgagaata
    13201 cacttccaat gactatgatt gataaaataa taattgatat atatggtaat tctttaattt
    13261 ttttagataa ggttttcttt gtttctatat gttcatcttc tgctactaaa atataatttt
    13321 ctcctacaat ttcaaaatca ctatattgtg gcttcattag tatcacctct atcatatttc
    13381 attctagggt ctattttatt gtttataatt tgagctaaca tattcccaaa tactactaaa
    13441 gcccctgtta ttaagcataa aactaataac atattataat catgatactt tgcactttca
    13501 aaacaaagtg ttcctaaccc aggataagaa aaaacttttt caactacata agttcctcct
    13561 agtatatgag gtatcgaaat ggccattata ctaatatatg atggcattat atttctcagg
    13621 caatgcttaa aaacaatagt tctattattt aaaccttttg ctttacataa aagcacataa
    13681 tcttctctta tttcctctag taatttattt cttatcatat atgtgtaata ccatagatgg
    13741 cttaatataa gtactgtaag aggtaagatt agatgtgata tcctacttaa tatactggac
    13801 tcttctccca ttgaataagc accactactt ggaagtatac ttaagtttat actaaatata
    13861 agtatgagta ctaaggcaac ccaaaatgat ggaatacagt tggttattgt tcctaatttg
    13921 catatgattc tatcaatgag cttatcttca tgtaatgtac aaaatattcc taacaataag
    13981 gctagcacaa aggtaagtat atatcctgaa ccacctaata ttatagtgtt tatatagaca
    14041 tcatttataa cagaagtaac attttgttta tatttaaatg aaattccaaa ttcccctttt
    14101 gatgcatttg aaatccattt tatatattga ctgtatattg gttcatccaa accaagtttt
    14161 ttcatagcat tttctttttc ttgtgtactc attctttcta ctccatcccc atagtaagag
    14221 attaatgggt ctcctggagc aagtcttgcc atgtaaaaaa caataaaaga taataaaaac
    14281 attactaaaa taaactgcaa caactttttc agtatatcct ttaacaaacc ccaacccccc
    14341 tttgttgcaa taaaaaaaac cacataaaag caaacgtaaa gtaacgttaa aaccttcgtg
    14401 gttccattat cattaaacaa aattaaattt tctatattta tctatgagtt aattttagca
    14461 tcatttatta actgtgtaaa caaacttttt aaaaattgtt aaaatttcat caaattattt
    14521 tatttatacc taaaataaaa tttttatatt ctattcttca ttattttcta taaaattata
    14581 aaattttagg ttgactatat aagaaaagta tattatatta gtttgtatgc aaaccattta
    14641 agaggtgatt ttattgaaaa gtaattatga ctgttttaga atagccatgc tcttaaaaga
    14701 actttattct aaaactatgt acacagtaga agaaaatttt aaagaaaatg gattaacaca
    14761 ccaacagatt atagttataa aactgatagc tcataatcaa gaacttacaa tatcacaact
    14821 atgtgatgaa atgtctttag ctaaaggtac ggtttcaggc ataatcagca gattagaaca
    14881 gattggatat atagaaaaat tcaagaaatc taatgataaa agaaatacat acgttaagtt
    14941 tacaacaaca ggatttgaat ttgccacaaa ttttaaaatt aaaatgcaag aaagttttga
    15001 tgatattttt aaaaattgtg atgaaaacga attgagtgac ttagtaaaaa atcttagaaa
    15061 tatattggca aaagtaaagg agagataatt atgaattata aattaatact tgcaatagta
    15121 tttataacat cagctttaat tttctacact ataggtgtat ttggagaaag aaaggcaaaa
    15181 attttaaaaa agaaacatgt aattatattt tggcttggtt ttatatttga tactttagga
    15241 acatttacaa tgagtaacat agccaacagc catacttttg aagtaaaatc tgctttatca
    15301 caaaaccttc atagtattac aggtcttctt gcaattgtgc ttatgctttt tcatgcaagt
    15361 tgggctactt ttgtcctata caaagatgat gaggaaaaga aaaaattctt tcataagttt
    15421 agtatagttg tatggactat atggttagtt ccatatttta taggtatgtt tattggtatg
    15481 gctggatagt aaaataaaaa gtgtgtaaga aatactgtag gtaaatttgt ataagacaaa
    15541 tttatctaca tccttctttc acacttttac atatttattt ataatactcc attatttctt
    15601 tcatgtttct taatacctat atagaatatt taattgtttc tatagtattt tattattttc
    15661 tcacactttt tcatatat
//
LOCUS       DAMJYR010000089        15381 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.091, whole genome shotgun sequence.
ACCESSION   DAMJYR010000089 DAMJYR010000000
VERSION     DAMJYR010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15381)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15381)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15381
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.091"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            93..1484
                     /locus_tag="PC471_002664"
     CDS             93..1484
                     /locus_tag="PC471_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HDF2789781.1"
                     /translation="MSSTESVSKKSGHPKSFWLICMTILWERFSYYGLTALLVLFFTA
                     SVAQGGLGLSTQEATSMFGLFTGLIYLTPLVGGWLADKVLGQQKCISIGALLIAIGDF
                     VLFASSTSIAMVWVGLSIIILGNGFFKSSCSNIVGDIYPKTDVSRKDAAYSLFYMAVN
                     VGAFIAPIICGLLSDNVFAVRGASGDIVSYGYKMAFLICGIGMMLGMIIFTLLAPKLL
                     NEVGKYPVTKGNKGEKIEYGPLTKLEKNRIIAMVILFLFVVLFWTAYYQTQSSFMIYV
                     RDNVNRTLFGFEMPVAWLTSLNAILCVALCPVLATLWVKLSHTKRGDFSIPVKMGLGM
                     IIMGVGFIVMVFATLANGPDGMVKASILFIVLTYIFTTVGELFLSPIGLAMFNKLAPA
                     KYATLSMGAWYLTSFFASLLSGKIAGFTATLGFLQIFGGIATVLIIFGLILLGIRNVL
                     TRLMAMDLLTKEK"
     gene            1737..3530
                     /locus_tag="PC471_002665"
     CDS             1737..3530
                     /locus_tag="PC471_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HDF2789782.1"
                     /translation="MNIKDRLSGLRKFMEEKNIDAYMIPSSDNHQSEYVGDYFKSREF
                     ISGFNGSAGTVIVTKDEAGLWTDGRYFIQAESQLEGSTIKLFKMGQEGCPTTDEYLYK
                     NIPEGGTLGFDGRVISAREGATLAEKLSKKGIKIEYQYDLIDSIWPDRPALSDSKAFL
                     LDVKYCGESFSSKLARLREKMSEKGTSTHVITTLDDIAWLFNIRGGDVKYNPVVLSYA
                     VITLKEVYLFVDESKLNEEILNELAKENVQIKPYNDVYEFVKNIDKTEKVLLDGTKLS
                     YTIYNNIPCEVEKVDEFNPVMFFKAQKNEVELENIRNSHVKDGVAFTKFMYWLKKNVG
                     KMEITEISATQKLEDLRREQEGFFEPSFNTIAAYKEHAAMMHYSATPESNYKLEAEGL
                     FLVDSGGQYYDGTTDITRTTVLGPISDELKLHFTSVARGMINLSKAKFLHGCRGYNLD
                     ILSRSCMWNMGIDYQCGTGHGIGFVLNVHEAPNGFRWRVVPERFDSAVLEEGMVTTNE
                     PGIYIEGSHGIRTENEIVVRKAEKNFYGQFMEFEVVTLAPIDLDGIAPELMNKDEKDY
                     LNWYHKLVYDKISPFLTDEEREWLKVYTRAI"
     gene            complement(3611..4993)
                     /locus_tag="PC471_002666"
     CDS             complement(3611..4993)
                     /locus_tag="PC471_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HDF2789783.1"
                     /translation="MSVLKTQEKLKQPRDLYFCCSIFTFVGLAYYGMKLVLLLFLAEQ
                     VSKGGLGLTPAESASMLASFLAWTYFSPVIGGWVSDRFLGPKNCIILGMLMVSIGYFL
                     GYIANSKMDINIMIFLSGLGIGFYKGNLHTMVGNLYTNDDPRKDGAFSITYMATNLGT
                     LFGPLICGLVANEWFAMKSGLSISMYGYRYVFLFSSITVLIGLFFFIFGNRKFYKPLN
                     NDPINGRESIRKSLEYKKMLASRPLTLIEKKRIIVILVLAFFTVFFWIAYNQASMSIA
                     LYTKEHINLTVGNFQIPTSWIDSYNGLLCVILGPISALVWVKLSQTKKGDLSVPKKMS
                     LGFILLAIAFLFMIVAVIQTGTDPNSIHKASVFWVVGFLTFQSIGEICFSPVGYGMVN
                     KLSPEKYISFLMGVWFLGKFAANKLSGYTQAIIDQLGMLQVFIVIPSFLLIFGIILLI
                     MNKKLVELSN"
     gene            5251..5598
                     /locus_tag="PC471_002667"
     CDS             5251..5598
                     /locus_tag="PC471_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HDF2789784.1"
                     /translation="MIRKKSELQVEVKENLNGGIGKVKLENIIQNEELKGKGRLFKRV
                     ALPVGSSIGVHDHTTDFEVYYILKGKGKVFDNGEFVEVNEGDVVYTADGEKHSIENIG
                     EEDLEFVALVLYM"
     gene            5616..6650
                     /locus_tag="PC471_002668"
     CDS             5616..6650
                     /locus_tag="PC471_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2789785.1"
                     /translation="MTGKIEEIKDKINRFCNSNLNQEYKDISFKILQNLLDNNKEIIH
                     SSRADIWSSAILNIVLEQNLLYNKKHPLHITKKEFSKQIGVSLNTINNKSSLIKDVCS
                     IDFLNQDTIAISNVEFWVKETNSKSKSVDLDLEKKKILYKRYIKLSQDANSDIESIRY
                     MEEAVNIGKSMITKEDKELGFWKGLSTRAYMIALESLARKLESINNLKESKKVLEYLI
                     EINPSDEQGIRYKLLNVLIRLNDRTSINKLFELYKEEKSATWMYSKALYYFKNKSMFL
                     ANDSIKVARSKNKYVGLYLLDWRNAFGREFVTEEEKAEAVYYYDENIVIWNEVKGSMD
                     WLLKKMLEFS"
     gene            complement(6699..7748)
                     /locus_tag="PC471_002669"
     CDS             complement(6699..7748)
                     /locus_tag="PC471_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDF2789786.1"
                     /translation="MEINYKKRNFKSFAKRKNIEISVQRYLIDALSYMALGLFSSLLI
                     GTIFNTLGDNFNISLFTEVISPLAKQVTGPAIAVAIAYGLQAPPLVLFSCALVGACGN
                     ELGGPVGAFVATLFAVEVGKLVSKETKIDILVTPAVTILIGVLVAELIGPAVSAFMTS
                     FGNIIMQATTMQPIIMGALVSALVGIALTLPISSAAICMMLSLGGLAGGAATVGCCCQ
                     MVGFAVMSFRENGVGGLVAQGLGTSMLQVPNIIKNWKIWIPPTVASILLGPLSTTIFK
                     MENIPIGSGMGTCGLVGQFGTVTAMQSAGKGGISMWVGILLLNFILPAIVTLLISEFM
                     RKKGLIKPEDLKLDV"
     gene            8023..9558
                     /locus_tag="PC471_002670"
     CDS             8023..9558
                     /locus_tag="PC471_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2789787.1"
                     /translation="MIFVKNKKYRKIAIIISIPILLVAQYYLFKFGILRPMVKGVEIE
                     IVDGEYVKDIDKYVVKLNETVTLSTGEYIKVPSYAKNPNIWFNVLDNSGTLKIDGNKM
                     TALKVGYSSVGIMKNSRVLKKATIKVVDPEVESLDMEINGDLKHVGDSATIESTIEVD
                     YDKFKKSYKPVYKSSNENILKIKGNKVSAVGVGKATIYAKCGNKEIERTFRIQARVAE
                     INMDDIKIEVDQNEKLKPDIITSPPGLEPPKIYYELVDSKLPVERAISLSSNGNVVGL
                     REGKDRVRITCGDKSKVITVTVVKETITNDYIQNLVGSHKIVNNKVIIKLEWDYMKDV
                     FDYDIYLKDKLSGESFKKIKSITINESDLGKSNKVYATIEVDMKGNQNIDFDIYVIGK
                     KDSDTTKPSNVINITNGNGNSNNDNNIKDMRVENISANVDRDNNVIRVNWSSISHKDC
                     TYSIYVKNNTNGDGEFTLYQSDIQGNEHTISIPEGDLNLDIYVVANYNGNSSQNSDVI
                     NVR"
     gene            complement(9743..10429)
                     /locus_tag="PC471_002671"
     CDS             complement(9743..10429)
                     /locus_tag="PC471_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HDF2789788.1"
                     /translation="MKTIGLIGGMSWESTITYYQVINTVIKERLGGLHSSKCILYSVD
                     FQEIEECQSSGNWEKSAKILADAAIKLQEAGADFIVICTNTMHKVSDKIQESIHIPLL
                     HIADVTATVLREKEIKKVALLGTKYTMEQDFYKNVIINNGIEVLIPNEEDRIIVNDTI
                     FNELCLGIISESSKKAFLSIIDKLGKQGAEAVILGCTEIGLLIKQNDTSIPLFDTTVI
                     HAIEAALSSI"
     gene            10554..11936
                     /locus_tag="PC471_002672"
     CDS             10554..11936
                     /locus_tag="PC471_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HDF2789789.1"
                     /translation="MPINSFENYPMTWKPKLDNRKPPIYKTLAMLLEEDIKRGNLNPG
                     DKLPPQRELADFLDLNLSTITRAFKLCEEKGLICAKVGKGTFISSDVNVSNTLLYQTE
                     SKDIIELGTVHPPYEQNTYIIDFIKNVLKQPEMDRFLQYMSPSGTYMQKKSIAKWIER
                     NNVYTSEENILLSTGGQNAICATLLGLFKAGDRIATDSLSFSGIKSIAKMIGIQLVPI
                     PQENNEISIEYLESYCKNENIKGIYLIPDYHNPTTHTMSDLSRKKIAKIAKQYNIIII
                     EDAINSIFRDGIQTPIFSLASDNTIYIFSTSKFLCAGLRVAFVVAPKRYIENLENALY
                     NMNLMVSPFTAEIVHRLLYSPIIDKIIEEKRDAIIERNEVADKVLSDYNLIGDKNCSF
                     RWLLLPDELDGKSFEISAKNLGVQVYCAERFSVGNSTVPKAVRICVTAPKDVEELEKG
                     LNIIKSLLKS"
     gene            12338..13606
                     /gene="eam"
                     /locus_tag="PC471_002673"
     CDS             12338..13606
                     /gene="eam"
                     /locus_tag="PC471_002673"
                     /EC_number="5.4.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 2,3-aminomutase"
                     /protein_id="HDF2789790.1"
                     /translation="MNEQTRISLERAAELKSKIDDYIQARKTINRGLEKEEEINKRKQ
                     KILSILNGTEEDWNNYKWQLSNRITDVDTLSKIITLTKKEKEYIKEVGTQFRWAISPY
                     YLSLIDPEDTCDPIKLLSIPTHIELEDEQEDLDPMGEEYTNPAGCITRRYPDRLIINV
                     TNECAMYCRHCQRRRNIGQQDSHKSKAIIQESIDYIRENEEIRDVLVTGGDALTLKDD
                     YLEWILSQLKEIPHVDYVRLGTRTLVTMPQRITDEFCNMLKKYHPVYINTHFNHPMEI
                     TKESKEACEKLANAGVPLGNQAVLLNGINNDKFVMRCLNQELLKIRVKPYYIFQSKHV
                     KGTKHFNTSVDDGLEIMEYLRGYTSGMAIPTYIVNAPKGGGKTPLLPQYLVSKGTDYV
                     MLRTWEGKVIKMEDEPAVDIKKLIKEQAQD"
     gene            complement(13699..14055)
                     /locus_tag="PC471_002674"
     CDS             complement(13699..14055)
                     /locus_tag="PC471_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2789791.1"
                     /translation="MKKALISLLVLSCLLLTAPVNESSYADSQKNVPVSQNTKLTQEQ
                     AKELLVKYNNEVDYIYQGNASDFEALKAKNLNGYVFLPDADGDIGYFVNENTSEIYYF
                     HPSGYLELVNQQQNNK"
     gene            complement(14206..15300)
                     /locus_tag="PC471_002675"
     CDS             complement(14206..15300)
                     /locus_tag="PC471_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HDF2789792.1"
                     /translation="MQDKKIYLNIVLTVVVSYILIKVIDNYKYFFGVISLLMSLLTPF
                     IIAFVLAYIFNPLVKFLESKLNFKRIYSLLLTYGVLIVILISFILFTVPSIVNSLADL
                     VAQIPTYIDKTEQFLFDLGKSLQSVDPNTLKEYGDKIMSVMPKFSNLLIGSLGGIFST
                     TFSVGKFIVQFLLGFIICFYILLEKEKFLLFTKKVVYISLGKKYGDFIIELCQSLNLN
                     IGKYFTGKILDSFIVGVLSGIGLYFLKSEYALLFGTLMGVMNMVPYFGPVIGMAPVVI
                     INLFSNPTIALTSLIYLIIIQQVEVTFIEPKIVGGQLGLSPFFTILAVTVGGGFFGIP
                     GMILSAPVMGVIKIYFCEFVNRRHDKIQME"
BASE COUNT         5940 a         1913 c         2484 g         5044 t
ORIGIN      
        1 catagaaaag atgatattat aagtatgtgc taaaatggtt aaagcaatat taatattaaa
       61 tttaactata aaacaaaagg gggcgttttt ttatgtcaag tacagaaagc gtatctaaaa
      121 aaagtggtca tccgaagagt ttttggctta tttgcatgac aattttgtgg gaaagattta
      181 gttattatgg tttaacagct ttattggtat tattttttac agcaagtgta gctcaaggtg
      241 gtttaggtct ttctactcaa gaagcaacat ctatgtttgg cttatttaca ggtcttatat
      301 atcttacacc attagtcggt ggctggttag cagataaagt tttaggtcaa caaaaatgta
      361 tttctatagg tgcactttta atagcaatag gagatttcgt actatttgct tcatctacat
      421 ctattgcaat ggtttgggta ggtctatcaa taattatttt aggaaatggt ttttttaaat
      481 caagttgctc aaatatagtt ggggatatat atcctaaaac tgatgtgtca agaaaagatg
      541 cagcatacag tttattctat atggcagtaa atgtaggagc atttatagct ccaataatat
      601 gtggattgct ttctgataat gtatttgcag taagaggagc aagtggagat atagtatctt
      661 atggatataa gatggcattt ttaatttgtg gtataggcat gatgttaggt atgattatat
      721 ttacacttct tgcaccaaaa ttattaaatg aagtcggaaa atatcctgtg actaaaggta
      781 ataaaggtga aaaaattgag tatggaccac ttactaagtt ggagaaaaat aggataatag
      841 caatggttat tttattttta tttgtggtct tattctggac agcatactat caaacacaaa
      901 gttcttttat gatttatgta agggataatg taaatagaac tctatttgga tttgaaatgc
      961 cagtagcatg gcttacatca ctaaatgcta tattatgtgt ggctttatgc ccagtattag
     1021 ctaccctatg ggttaaatta tctcatacca aaagaggtga tttctctatt ccagttaaaa
     1081 tgggattagg aatgataata atgggtgttg ggtttatagt tatggtattt gctacattag
     1141 ctaatggtcc agatggaatg gtaaaggcta gtatattgtt tatagtattg acttatatat
     1201 ttacaacagt aggtgaatta ttcttatctc ctataggact tgcaatgttc aataaattag
     1261 ctccagctaa gtatgctacg ttgtctatgg gagcctggta tctaacaagt ttttttgcaa
     1321 gtttattatc aggaaagatt gctggtttta cagccacact tggtttttta cagatatttg
     1381 gaggaatagc aacagtttta ataatatttg gtttaatatt attgggaata agaaatgtat
     1441 taacaagatt aatggctatg gatttactaa caaaagaaaa ataattcatg acgaattatt
     1501 gtaaataaat gaattacttt aaataaagga agttacattt aaatataaaa ttaaatgtaa
     1561 tttcctcttt gattatgaaa aaaagcttta cacaaataga tattataatt aaaattataa
     1621 ttgtgataat tagagtgtat aagatttata taaattatac aaaaaatatt tatgtaataa
     1681 actttacata cttatatatt tagttattca aactattatt tcgaggaggt cttgttgtga
     1741 atataaaaga tagattaagt ggattaagaa aatttatgga agaaaaaaat atagatgcct
     1801 atatgattcc atcttctgat aatcaccaaa gtgaatatgt aggagattac ttcaaatcaa
     1861 gagaatttat atcagggttt aatggttcag cagggacggt tattgtgact aaagacgaag
     1921 ctgggttatg gacagatgga agatatttta tacaggcaga atctcaatta gaaggaagta
     1981 caataaaatt attcaaaatg ggacaagaag gttgcccaac tacagatgaa tatttatata
     2041 aaaatattcc tgaaggagga actttaggat ttgatggtag agttatatct gctagagaag
     2101 gagctacatt agctgaaaaa ttatctaaaa aaggtataaa gatagaatat cagtatgatt
     2161 taatagatag tatatggcca gatagacctg ctttatcaga tagtaaagca tttttattag
     2221 atgttaaata ttgtggagaa agttttagct caaaattagc tagattaaga gaaaaaatga
     2281 gtgaaaaagg tacttctact catgtaataa ctactcttga tgatatagca tggttattca
     2341 atataagagg tggagatgtt aaatataatc cagttgtact ttcttatgca gtaataactt
     2401 taaaagaagt ttatctattt gttgatgaaa gtaagttaaa tgaagaaata ctaaatgaac
     2461 tagctaagga aaatgttcaa ataaagccat ataatgatgt gtatgaattt gtaaaaaata
     2521 tagacaaaac tgaaaaagtt ttattagatg gaactaagct tagttataca atttacaata
     2581 atataccttg tgaagttgag aaagttgatg agttcaatcc agtaatgttt tttaaagctc
     2641 aaaaaaatga ggtagaatta gaaaatataa gaaattcaca tgtaaaagat ggtgtagctt
     2701 tcactaaatt tatgtactgg ttaaagaaaa atgtaggtaa gatggaaatc actgaaataa
     2761 gtgctactca aaaattagag gacttaagaa gagagcaaga aggtttcttt gagccaagtt
     2821 tcaatacaat tgctgcatat aaagagcatg ctgcaatgat gcactatagt gcaactccag
     2881 agtcaaatta taaattagag gcagaaggat tatttttagt agattcaggt ggacaatatt
     2941 atgatggaac tactgatata actaggacta cagttttagg acctataagt gatgaattaa
     3001 aactacactt tacttctgta gcaagaggaa tgataaatct ttctaaggct aagttcttgc
     3061 atggttgtag aggatataac ttagacatac tatctagaag ttgcatgtgg aatatgggaa
     3121 ttgattatca atgtggaact ggacatggta taggatttgt gctaaatgtt cacgaagctc
     3181 caaatggatt tagatggaga gtagtacctg aaagatttga tagtgcagtt cttgaagaag
     3241 gaatggtaac tactaatgaa ccaggtatat atatagaagg ttctcatggt ataagaacag
     3301 agaatgaaat tgtagttaga aaagctgaaa agaatttcta tggtcaattt atggaatttg
     3361 aagttgttac gttagctcct attgacttag atggtatagc accagaatta atgaataagg
     3421 atgaaaaaga ttatttaaat tggtatcata agttagttta tgataaaata tcacctttct
     3481 taactgatga agaaagagaa tggttaaaag tttacactag agctatatag tttgtactag
     3541 atacaataaa aatcgaggta ttataatgcc tcgatttttt attgtatatt ttattaactt
     3601 taaaaaattt ttaatttgat aattctacta attttttatt cattataagt agtataattc
     3661 caaatatcaa taaaaatgat ggtatcacta tgaagacttg taacatacca agttggtcta
     3721 taatagcttg cgtatagcca gataatttat ttgctgcaaa tttgcctaaa aaccaaacac
     3781 ccattaggaa tgaaatatac ttttcaggcg aaagtttatt taccatacca tacccgactg
     3841 gagaaaagca aatttctcct atactttgga atgttaaaaa tccaactacc caaaatacac
     3901 ttgctttgtg aattgaatta gggtctgttc ctgtttgtat aactgctact atcataaaca
     3961 aaaatgctat ggctaatagt ataaaaccta agctcatttt ttttggcaca cttaaatcgc
     4021 cttttttagt ttgagataat tttacccata ctaaagcaga gataggtcca agaataacac
     4081 aaaggagacc attgtatgaa tctatccatg aagtaggaat ttgaaaattg ccaacagtaa
     4141 gatttatgtg ttcctttgta tagagtgcaa tagacataga ggcttggtta tatgctatcc
     4201 aaaaaaatac tgtgaaaaaa gctaacacca atattactat tattcttttt ttctcaatca
     4261 aagttaaagg tcttgaagcg agcattttct tatattctag tgattttctt atagactctc
     4321 tcccgttaat agggtcatta ttcaaaggtt tataaaactt tctgtttcca aatataaaga
     4381 agaataaacc tattagaacc gtaattgatg aaaataaaaa tacatatcta tatccataca
     4441 tacttataga taatccgctt ttcatagcaa accattcatt agcaactaaa ccgcatatta
     4501 aagggccaaa taaagtacct agattagtag ccatatacgt aatagagaaa gctccatctt
     4561 ttcttgggtc atcattggta tataagtttc caaccatagt atgtaagttt cctttataaa
     4621 aacctattcc taatccagat aaaaatatca tgatgtttat atccatctta ctatttgcta
     4681 tgtatcctag aaaataacca attgaaacca ttagcattcc aagtataata cagtttttgg
     4741 gacctaaaaa tctatcactg acccatccac caataactgg agagaaataa gtccatgcta
     4801 aaaaagatgc aagcattgat gcggattctg ctggtgttaa ccctaaacca cctttggaga
     4861 cttgttcagc taaaaacaat aataatacca atttcatacc atagtatgca agtcctacaa
     4921 aggtaaatat tgaacagcag aaatataaat ctctaggttg tttaagtttt tcttgcgttt
     4981 ttaaaactga catatgtcga gaacctccag ataaatagtt taacaaaatt ttaccatact
     5041 atatgagtat atgttttttt catatagacg tcaacttgta agataattta attaagtcga
     5101 aatatatgta aaaatagtat catttttgtg tattcagtaa tattttttta tgataatata
     5161 tgttattatt atattaaaat acaaacatat ttatggaaag gatatgccaa tttagtagtt
     5221 agattgattg tttggcaggt gggatgagct atgataagaa agaaatctga attacaagta
     5281 gaagtaaaag aaaatttaaa tggtggtata ggaaaggtta agcttgaaaa cataatacaa
     5341 aatgaagagc ttaaaggaaa aggaagacta tttaaaagag tagctttacc agtaggaagt
     5401 tcaattggtg tgcatgacca tacaacagat tttgaagtat attatatact aaagggaaaa
     5461 ggtaaagtat ttgataatgg ggaatttgta gaagtaaatg agggtgatgt agtgtataca
     5521 gctgatggcg aaaaacattc tatagaaaat ataggagaag aagatcttga gtttgtagca
     5581 ctagttttat atatgtaaaa tatggaggag gagtcatgac aggcaagata gaagaaatta
     5641 aggacaagat aaatagattt tgcaactcca atttaaatca agaatacaaa gatataagct
     5701 ttaaaatttt gcaaaacctt ttagataata ataaagagat aattcattct tctagagctg
     5761 atatatggtc ttcagctata ctaaatatag ttttagaaca aaatctttta tacaataaga
     5821 agcatccact acatataaca aaaaaagagt tttcaaaaca aataggtgtt tctttaaata
     5881 ctataaataa taaatcgtct ttaataaaag atgtttgtag tattgacttt ttaaatcaag
     5941 atactatagc tatttctaat gtagaatttt gggtaaaaga gactaattcc aaatcaaagt
     6001 ctgtagattt agatttagaa aagaagaaaa tactatataa aagatatatt aagctatcac
     6061 aagatgcgaa tagtgatatt gaatcaatta gatatatgga ggaagctgta aatataggta
     6121 aaagtatgat tactaaagaa gataaagaac ttggattttg gaaaggttta tctaccagag
     6181 cctatatgat agcacttgaa tcccttgcta gaaagttaga aagtattaat aatctaaagg
     6241 agtccaaaaa agtattagaa tatttaatag aaattaatcc tagtgatgag cagggaataa
     6301 gatacaaatt attaaatgta ttaattagat taaatgatag aacttcaata aataaattat
     6361 ttgaacttta taaagaagaa aaatctgcta catggatgta cagtaaagct ctttattact
     6421 ttaaaaataa aagtatgttt ttggcaaatg attctattaa ggttgctaga agtaaaaata
     6481 agtacgttgg attatatttg ttagattggc gaaatgcttt tggtagagaa tttgttacag
     6541 aggaagaaaa ggcagaagct gtctattact atgatgaaaa catagtgatt tggaatgaag
     6601 ttaaaggttc tatggattgg ctattaaaaa aaatgttgga attttcataa aaaaaactac
     6661 tccttacttt tatataagtc aaaggagtag tttttaattt atacatctag ttttaaatct
     6721 tcaggtttta ttagaccttt ttttctcata aattctgaaa tcaacaaagt tacaatagca
     6781 ggaagtataa aattaagtaa aagtatacca acccacatag atatacctcc ttttccagca
     6841 ctttgcatag cagttactgt tccaaattgt ccaaccaatc cacaagtacc cataccagaa
     6901 ccaataggaa tattctccat tttgaatatt gttgtagata atggtccaag taatattgaa
     6961 gctacagtag gtggtatcca aattttccaa ttttttatta tattaggtac ttgcaacata
     7021 gatgtcccaa gaccttgtgc cacaaggcca ccaactccat tttctctaaa gctcataaca
     7081 gcaaagccta ccatttggca acaacaacct acagttgctg caccaccagc aagaccacct
     7141 aaacttaaca tcatacaaat agctgcacta cttataggta atgtaagagc tattccaaca
     7201 agtgcagaga ctaaagctcc cattattata ggttgcatag ttgtagcttg cattattata
     7261 tttccaaatg aagtcataaa agcagaaaca gctggaccaa taagttcagc tacaagtact
     7321 ccaattagta ttgttacagc aggtgtaacc aatatatcta tttttgtttc ttttgaaact
     7381 aattttccaa cctctacagc aaaaagtgta gcgacaaatg ctccaacagg tcctcctaat
     7441 tcattaccac aagctccaac aagtgcacaa gaaaatagta caagtggagg tgcttgaagt
     7501 ccatacgcaa tagccactgc tatagctgga ccagtaacct gtttagcaag agggcttata
     7561 acttcagtaa aaagagaaat attgaaatta tctcctaatg tattgaaaat tgttccaatt
     7621 aaaagggacg aaaaaagacc gagtgccata taacttagtg cgtcaataag atacctttga
     7681 acagaaattt caatattttt tcttttagca aatgatttga aatttctttt tttataattg
     7741 atttccaatt taaaatcctc ctcatataat ataagtataa tttcttttta attaattata
     7801 taacttttca gctgacaaga caaatgttat ttcactaaaa ttagactatt gtaatagtaa
     7861 gaaattaaca ttaattttag ttttaaattt taaatttatg gtttataatt agtataggga
     7921 tattctgaaa tataataatt ttccagttta tcccaatatt taacttaggt agattgctta
     7981 caaaacaaat gattatatat taggctaaaa ggaagtggat agatgatatt tgtaaaaaat
     8041 aagaaataca gaaaaattgc cataattata tctattccta tattgcttgt tgcgcagtac
     8101 taccttttta aatttggaat acttagacca atggtaaagg gagtagaaat tgaaattgta
     8161 gatggggaat atgttaaaga tatagataaa tatgtagtaa aattaaatga aacagttact
     8221 ttgtctacag gtgaatatat taaggttcct tcgtatgcta aaaatccaaa tatttggttt
     8281 aatgtattgg ataactcagg gactttaaaa atagatggaa ataaaatgac agcattaaag
     8341 gttgggtatt cttctgtggg tatcatgaaa aattcaagag tattaaaaaa ggctactata
     8401 aaagtagtag acccagaggt agaatcacta gatatggaga ttaatgggga tttaaaacat
     8461 gttggtgata gtgctactat agaaagtaca atagaagttg attatgataa gtttaaaaaa
     8521 tcatataagc cagtttataa gagtagcaat gaaaatattt taaaaatcaa aggaaataaa
     8581 gtaagtgcag ttggagttgg taaagctaca atatatgcta agtgtggaaa taaagaaatt
     8641 gagagaacat ttaggattca agctagagtt gctgaaataa atatggatga tataaaaata
     8701 gaagttgacc agaatgaaaa attgaaacca gatataataa cttctccgcc tggtttagaa
     8761 ccaccaaaaa tatattatga gttagtagat tcaaaattac ctgtagaaag agcaataagt
     8821 ttaagtagta atggaaatgt tgtagggctt agagaaggta aagatagggt gagaataact
     8881 tgtggagata aatctaaagt aataacagta acagtagtca aagaaactat aacgaatgac
     8941 tatatacaaa acttggttgg ttcacataaa atagtaaaca ataaagttat tattaaactt
     9001 gaatgggatt atatgaaaga tgtctttgat tatgatatat atcttaaaga taagttgtca
     9061 ggagaatcat ttaagaaaat taaatctata actataaatg aaagtgattt aggtaagtct
     9121 aacaaggtat atgcaactat agaagtagat atgaagggta atcaaaatat agattttgat
     9181 atttatgtca tagggaaaaa ggattcagat acaactaagc caagtaatgt aataaatatt
     9241 accaatggaa atggaaattc aaataatgat aataatatta aagatatgag ggtagaaaat
     9301 atatcagcta atgttgatag agataataat gtcattagag ttaactggag tagtatatct
     9361 cataaggact gtacttacag tatatatgta aaaaataata ctaatggaga tggagaattt
     9421 actctatatc aaagtgatat acagggaaat gagcatacta taagtattcc tgaaggagat
     9481 ttaaatctag atatttatgt tgttgcaaat tataatggaa atagctctca aaatagtgat
     9541 gttataaatg taagatagat ttgtatacaa attgaaagtg ttgagtttat ttaaatatta
     9601 aattaagaaa aaggatagta tagggtaatt atactatcct tttttgttac aatatatagg
     9661 tttaaaatct ctagtaatat aaattataaa aagtcttatt tacacaaatc tatagatatt
     9721 ttttttatta atcaatattg tcttaaattg aacttagtgc ggcttctatt gcatgtatta
     9781 ctgtcgtatc aaatagtgga atagaagtgt cattttgttt aattaaaaga cctatttctg
     9841 tacatcctaa tataacagcc tctgcacctt gtttgccaag cttatctata atagataaga
     9901 aagctttttt tgaagattca gatataattc ctaagcaaag ttcattaaat attgtgtcat
     9961 ttactattat acggtcttct tcatttggaa ttaagacttc aattccatta ttaataatta
    10021 catttttata aaaatcttgt tccattgtat atttagtacc taataaggcc acttttttta
    10081 tttctttttc ccttaatacc gtagcagtta catctgctat gtgaagtaat ggaatatgta
    10141 tactttcttg tattttatct gataccttat gcatagtatt agtacagata acaataaaat
    10201 ctgcccctgc ctcttgtaat ttaattgcag catcagctaa aattttggcg cttttttccc
    10261 aatttccact agattggcat tcttcaattt cttgaaaatc tacactgtaa agtatacatt
    10321 tagaagagtg tagtccacct aatctttctt ttattactgt attaataact tgataatatg
    10381 taatggtact ttcccaactc ataccaccaa ttaagcctat agtcttcata ttatacctct
    10441 cttatttatt tattttgaaa tagctattta caaatacaag tatagcatct aaaatagaag
    10501 catacaatga taaaattgta tggagtacaa ttataattaa gaggagtaat tatatgccaa
    10561 taaattcttt tgaaaactat cctatgacat ggaaaccaaa attagataac cgaaaaccac
    10621 caatatataa aactttagca atgttattag aagaagatat taaaagagga aatttaaatc
    10681 ctggagataa actgcctcca caacgggaat tagcagattt tctagattta aacttaagta
    10741 caataacacg agcatttaaa ctttgcgaag aaaagggact aatatgtgca aaagtgggaa
    10801 agggaacatt catttcttct gatgtaaatg taagtaatac attattatat caaacggaat
    10861 caaaagacat aattgaatta gggacagttc atcctccgta tgagcaaaat acatatatta
    10921 ttgactttat taaaaatgta ctaaaacaac cagaaatgga taggttttta caatacatgt
    10981 ctccatctgg cacatatatg caaaaaaagt caattgcaaa atggatagaa agaaataatg
    11041 tatacaccag tgaagaaaat atattattat ctacaggagg gcaaaatgct atatgtgcaa
    11101 ctttactagg attatttaaa gctggtgata gaattgctac agattcttta agtttttctg
    11161 gaattaagtc aatagctaaa atgataggta ttcaattagt cccaattcct caagaaaata
    11221 atgaaatatc gattgaatat cttgaaagtt attgtaaaaa tgaaaatata aaaggcatat
    11281 atcttatacc agattatcat aatccaacga cacatactat gtctgattta tctcgtaaaa
    11341 agatagccaa gatagcaaag caatataata taataattat tgaagatgct ataaatagca
    11401 tttttagaga tggtatacaa actcccatat tttctttagc tagtgataat actatatata
    11461 tttttagtac atctaaattt ttatgtgctg gtcttagagt agcatttgta gttgctccaa
    11521 aacgatatat agaaaattta gaaaatgcac tatataatat gaatctaatg gtttctccat
    11581 ttacagcaga aatagtacac cgtttattat attctcctat tatagataaa attatagaag
    11641 agaaaagaga tgcaattata gaaagaaatg aagttgctga taaagttttg tcagattata
    11701 atttgattgg tgataagaat tgcagttttc gttggttact tcttcctgat gaattggatg
    11761 gaaaatcatt tgaaatttct gcaaaaaatc taggagttca agtatattgt gctgaacgat
    11821 tttcagtagg aaactctaca gttccaaaag cagttcgtat ttgtgtaaca gctccaaaag
    11881 atgtagaaga attagaaaaa gggcttaata taataaagtc acttcttaag agttaaattt
    11941 attttactat tatttaatta aataaataaa aaggcggaga aatttattat aataaacctc
    12001 cgcttttatt atttaatgaa atactttact ttttattaaa aaatttaatt ttaataatct
    12061 tgaaaatata atcgttttat ggtaaaataa ttcttaaaaa tagaaaaaat taatcatttg
    12121 ttaaaaaaaa tgcataattt attgtaattt attgacgaag gttgaaaaat attcataaat
    12181 gtgcgaaata acagaaaaaa tgcaaaaatt aattcaattt catttgttat aaaatctata
    12241 tggcaaaatt aaatctgcct atcagaatga tataaacaca ccagaaagga ggacgctatc
    12301 aaataataca agtagttaac tttgatagca aaaaattatg aatgaacaaa ctagaatatc
    12361 cttagagaga gctgctgaat taaaatcaaa aattgatgat tatattcagg ctagaaaaac
    12421 gattaacaga ggtcttgaaa aagaagaaga gataaataaa cgaaaacaga aaatattaag
    12481 tatcttaaat ggaactgaag aggattggaa taactacaaa tggcaattat caaatagaat
    12541 aacagatgta gatactttat caaaaattat aactctaact aaaaaagaaa aagaatatat
    12601 aaaagaggtt ggtactcaat ttagatgggc aatatctcca tattatttga gtcttataga
    12661 cccagaagat acatgtgacc caataaaatt actgtctata ccaacacata tagagttgga
    12721 agatgaacaa gaagatttgg acccaatggg agaagagtat acaaacccag caggatgtat
    12781 aactagaaga tacccggata gattaataat aaatgtaaca aatgagtgtg ctatgtattg
    12841 tagacactgt cagagaagaa gaaatattgg acaacaagat tctcataagt caaaagctat
    12901 tatccaagaa tctatagact atatcagaga aaatgaagaa ataagagatg tactagtaac
    12961 tggtggagat gctcttacat taaaagatga ttatttagag tggattctta gccaacttaa
    13021 agagatacca catgttgatt atgttagatt aggtactagg actcttgtta caatgccaca
    13081 aagaatcaca gatgaatttt gcaatatgct aaaaaaatat caccctgtat atataaatac
    13141 tcattttaat catccaatgg aaataactaa ggaatctaaa gaagcttgtg aaaagttagc
    13201 aaatgcagga gttccattag gaaatcaggc agtattatta aatggaataa ataatgataa
    13261 atttgtaatg agatgtttaa atcaagaatt actgaaaata agagtaaaac cttattatat
    13321 attccaaagt aaacatgtaa agggaacaaa acatttcaat acatcagtag atgatggtct
    13381 tgaaatcatg gagtatttaa gaggatatac atcaggaatg gctataccaa catatatagt
    13441 aaatgctcca aaaggaggcg gaaagactcc tttgcttcct caataccttg tatcaaaagg
    13501 aacagattac gttatgctta gaacatggga aggaaaagtt ataaaaatgg aagatgaacc
    13561 tgctgtagat ataaagaaac ttataaaaga acaagcgcag gattaaaaaa gtatttaaag
    13621 tttataaaaa gaaacaactt taaaataaaa agaaaatgga tgtcaaattt atatcaattt
    13681 gcatccattt tttattagtt atttattatt ttgttgttga ttaactaatt caagatatcc
    13741 acttgggtgg aagtaatata tctcagaagt attttcattt acaaagtaac caatatcacc
    13801 atctgcatct ggtaaaaata cataaccatt aagattttta gcttttaatg cttcaaaatc
    13861 actagcattt ccttgatata tgtaatcaac ttcattgttg tattttacta aaagttcttt
    13921 agcttgttct tgagtcaatt tagtattttg gcttacagga acattctttt gagaatctgc
    13981 ataagaagat tcatttactg gtgcagttaa taacaaacaa gataaaacta ataaagatat
    14041 aagtgctttt ttcatactaa aaccctcttt catgtttaat tatataagat tcattactta
    14101 ataatattgt atgtttccta taattaaata ttaccattaa agagaataat attacagtta
    14161 cacagctgta acttttgatt acaaaatcgt aattaaattg tatttttatt ccatttgtat
    14221 tttatcatgt ctacgattta caaactcaca gaagtatatc tttataacgc ccatcacagg
    14281 agcagataga atcataccag gtataccaaa gaatcctcca ccaacagtaa ctgctaggat
    14341 tgtaaagaat gggctaagac ctagttgtcc acctacgatt tttggctcta taaatgtaac
    14401 ttcaacttgt tgaattatta ttaagtaaat taaagaagtt aaagcaattg ttggattgga
    14461 gaataaatta attattacaa ctggtgccat acctattact ggaccaaaat atggaaccat
    14521 attcatgaca cccataagtg taccaaataa tagtgcatat tcagatttta aaaagtaaag
    14581 accaattcca gaaagtactc caactataaa agaatctaaa atcttacctg taaagtattt
    14641 accaatattt aaatttaagc tttgacatag ttctattata aagtctccat attttttacc
    14701 taaggaaata taaactactt tttttgtaaa cagtaagaat ttttcttttt ctaataatat
    14761 atagaaacaa attataaaac ctaaaagaaa ttgtactata aattttccaa ctgaaaatgt
    14821 agtgctaaat atcccaccaa gtgaaccaat aagcaagtta ctaaatttag gcattacact
    14881 cataattttg tcaccatact cttttaatgt attagggtca actgattgta aactcttgcc
    14941 taagtcgaat aaaaactgct ctgttttatc tatgtatgta ggaatttgag caactaaatc
    15001 agctaaacta tttactatgg atggaactgt aaataatata aacgaaatta gtataactat
    15061 gagtacacca taagtaagaa gtaacgaata tattctctta aaatttaatt tactttctaa
    15121 gaactttact agtgggttga atatatatgc taatacaaat gctattataa atggtgttag
    15181 tagtgacatt aataaactta tcactccaaa aaaatatttg taattatcaa taactttaat
    15241 caagatatat gaaacaacta cagtgagtac aatatttaga taaatttttt tatcttgcaa
    15301 aataacacaa cctttcttat atttattaaa tttagatata cttaaatcta agttaaagtt
    15361 aatatttata gcttattata c
//
LOCUS       DAMJYR010000090        14628 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.093, whole genome shotgun sequence.
ACCESSION   DAMJYR010000090 DAMJYR010000000
VERSION     DAMJYR010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14628)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14628)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14628
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.093"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            244..2886
                     /gene="adhE"
                     /locus_tag="PC471_002676"
                     /gene_synonym="adhC"
     CDS             244..2886
                     /gene="adhE"
                     /locus_tag="PC471_002676"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HDF2789793.1"
                     /translation="MEKKEKVVEKSNVEVCSPEIVNSVETLRMRLEEIRLAQKEFATF
                     TQEQVDKIFLAASTAANQQRIPLAKMAVEETGMGIVEDKVIKNHFASEYIYNAYKDTK
                     TCGVIEKDEAFGFTRIAEPVGVLSAVIPTTNPTSTAIFKSLIALKTRNGIIFSPHPRA
                     KNCTIEAARVVHDAAVKAGAPKGLIGWVDVPSIELTNVVMAEADLILATGGPGMVKSA
                     YSSGKPAVGVGPGNVPAIIDESADIKMAVSSILVSKSFDNGMICASEQAVIVPEKIYE
                     EVKKEFKYRGAHFLNKEETEKVGKVVIIDGSLNARIVGQPAHVIAKMADVEVPKTARI
                     IIGEVESVELNEPFAHEKLSPVLAMYKSKSFEDAVAKAEKLVADGGYGHTSSLYADSI
                     NHPERVEKFVNAMKTCRVLVNTPSSQGGIGDLYNFKLAPSLTLGCGSWGGNSVSENVG
                     VKHLLNIKTVAERRENMLWFRAPEKVYFKKGCLGVAAREFKDVMDKKKAFIVTDSFLY
                     NNGYTKKLTDLLDEMGIKHTTFFDVAPDPTLACAREGAKAMADFQPDLIIAVGGGSAM
                     DAGKIMWVMYEHPEVDFQDLAMRFMDIRKRVYVFPKMGEKAYFAAIPTSAGTGSEVTP
                     FAVITDQDSGVKYPLADYELMPNMAIIDADMMMEMPPRLTAASGVDALTHALEAYVSM
                     LRTEPADGLALQAGKIIFEYLPRAYKNGKNDKEAREKMAMASTMAGMSFANAFLGICH
                     SLAHKLGAFHHVQHGVANALLINEVIKFNCAEAPNKMGAFSQYRYPDCIQRYAEFASF
                     AGIKGSTDQEKVDNLIKAIDELKAKVGLPKTIKEAGVEESKFLERLDAMVEQAFDDQC
                     TGANPRYPLMSELKEIYLKVYYGK"
     gene            complement(3246..3587)
                     /locus_tag="PC471_002677"
     CDS             complement(3246..3587)
                     /locus_tag="PC471_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2789794.1"
                     /translation="MLLILGNLNLNLDFDYRIIREENEDVDIFIDINYRSLDIDTDGS
                     NLFNSRIQFPFVRALILRLNKNNQCMTIHLLRDIDLFSAFANFEVDYTDSIINIKNQN
                     EKVILNKSIKK"
     gene            complement(3614..4285)
                     /locus_tag="PC471_002678"
     CDS             complement(3614..4285)
                     /locus_tag="PC471_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2789795.1"
                     /translation="MPILETINLGKIYGKKETSVHALKNANLKINKGEFVAIIGPSGS
                     GKSTFLHLVGGLERPSNGTIKVDGKDICCLSDKELARYRRQKVGFVFQQYNLIPVLNV
                     KENIELPLKLDNKKIDKEYIDDLINLLGLNERKNHLPNQLSGGQQQRVAIARALSAKP
                     SIILADEPTGNLDSKTTEEVMDLLKSSIKKYNQTLIIITHNENIARKADRIISIIDGE
                     LKLTL"
     gene            complement(4297..6873)
                     /locus_tag="PC471_002679"
     CDS             complement(4297..6873)
                     /locus_tag="PC471_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HDF2789796.1"
                     /translation="MKDNLKIVLRYIKSYKARSLAIVVSIILATSLVVGIGTLSRSAQ
                     QADVDRLKRELGTDHVYFKNIKKNQLKSIKSSKDIKNLGITSYYASTDVNEKLPINIL
                     YTNKNYLTSHSKLIKGRFPKASNEVVVEKWILNSMGLKPEINQNLTFKLYQKENPETF
                     KVVGILEDRFIEKKKGQCEIFSNLNESELNDFTTYIEFNEGSDINTNIDNIIKNAMLD
                     EKSIGRNSMLIESTMKNGTLDNSSKYVAIAMSLFAGIVIYSIYVISIYQRVQEYGVLR
                     AIGSTNFRIFKLMLYELFILALIAMPIGICIGMGGAQIFNRSVGNIQFEGNINVTPFV
                     IPDKIILLSIACTILIILIISFFTYLKIRRISPIDAIRKTFGADKNVTKVNYLISKLT
                     LNMSVTKSISAKNIFRNKKGFIIIVLSMSIGGIIVIKDNYKYSFLDVQNKSTQEQTYM
                     NADFILSNVYLNEIGNNTSFKDIKGFNDNQIDKIKNIDGIDKVKTASILNTRIEVNKI
                     NRLDYYEAINSTPYYKLNPLFIKNKTTGKYTLKQKLKGYNDEMIKSLEKYLVSGNINL
                     KKMKKENLAIVYVPEISKTQKYTKTFTPGEGTPAVDIKVGDTIKVKYPKGGMDTGLYE
                     SLKDNYEYLEYEFKVGAIISYPFADNGMYSGDDGVDVITSSEYLKKLTGVDKYNLVYA
                     DLNENANKKKINNLLGKIGSEVPGTTTVDMMVEKENSDKMTSRTMIYSYGIVAIMFII
                     SVLNIINNISYNLTSRTSEFGMLRAIGISERGFKNMILYEGILYGVLSSIITIVSGII
                     IQFKMYYMQGFVSYGLGFSIDYKIYILVVVANIIVGILATYIPSRKINKISIVEAINI
                     TE"
     gene            7081..8016
                     /locus_tag="PC471_002680"
     CDS             7081..8016
                     /locus_tag="PC471_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HDF2789797.1"
                     /translation="MDNNFVFPIVILTLFIFSNLIMNKYKFYKELDILKRGILTFFIS
                     CFALILFMEGTKYIQLRLGYSFLLEGVLNIILFLVLIILSFYTGFKVIRLINNNNEQE
                     YKLQKLDSKINHKDNIIIKLRSQKHDYLKHLQVVYSLLNTGLDEDAKEYIMNISNSFK
                     SKNSRYGKVSYLDAIASLKYEECLEKDISFDVYIQDFMDNLEIEPNDMSSVLLNIIDN
                     AIESLSTIKKEKKYIRLHAYEDEFQYIIAIKNNGPKIHNVESIFWNGFSTKEGQDRGY
                     GLHLVKNILDKYDYSILVNSDDFLTEFVILIPKYY"
     gene            8035..8772
                     /locus_tag="PC471_002681"
     CDS             8035..8772
                     /locus_tag="PC471_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2789798.1"
                     /translation="MIKVLICDDDKIVRDDIANIAKESGYVDYVKKVKDGIEAIEFIK
                     NEKIDLLIIDIDMPYKNGIDSAEEILSIDKDIHIVFVTGFADYSLESFKVHPIDFVVK
                     PFKKEKILDSINIAIDHINSHKIANNNFIEDTLFVYKIRKQIHMINFDDIVMFEKNSR
                     AINLHTKDEDIIKFYENFDDLKKRLPPNFFMTHRQYIVNLRFIHKVIPINKSSLEIRF
                     INFQENAILNKSLEKDFLYRFYRVKRF"
     gene            complement(8957..14014)
                     /locus_tag="PC471_002682"
     CDS             complement(8957..14014)
                     /locus_tag="PC471_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
                     /protein_id="HDF2789799.1"
                     /translation="MLERTRVEKNPKIIENFKNNFTKSSSKQYYDLAKSLWDCIENSS
                     YYYREPEDKFLNMIKKYYNCHYYEIKNMDELLSGPLKNALELIFTSEQVEELCQLIKR
                     KMDYTYSGGYYRKGYRSNKLVDYFRGIIRFISNYVDLIYYDFSVIDYLTLPNKVLYYD
                     SIMSDKIALEIDKGNQKVISALEEVIFGENQTALLTRQMIIGIMKSHSDKCYEMLSKL
                     LLAAKQQEGLRQQIVESLDEGTISASIYMLKTILDNNLERFSSVIRALDTWTGLGFSD
                     QKPGIIRKCLELSYKSLTDEKFRIESINSNDNLEIYFALWATATYNIYDSYSLIENLL
                     SCQEKYKKLVALYFLSHTESTYFKHSIASKNIKGDDLEILAWVITNFYVEGSVGYYYS
                     WQNRETNFDLSNTPIDKDFDARKEQFESLKHTLEILNSKTKSFSGSVFPWTWIEISSH
                     EILKCMMTLTAYDMNAQFIDVLMENCHLMSSDLRASLIVNFVNEPKTDRQREFLLECL
                     SDKSVSNRETVLSLLRKLELTDVELDKITNLLSLKTSSIRQTVIKILLEQDDSNLENI
                     ASSLIKSSNSQKCLGGLEIVNLLKDNTARQELYNKLILLIEDIDKSKKNTSQVQILID
                     NLLGKAKNDYCKENGFGIYNPENKVQIPEQFDFKDDSNSSSKLNKIISNISLSTREDS
                     VSVATKKEAKKILDTDINKIIKVYEKFDELITENADYEYEVERWSGNVDKVLLGYSTY
                     NLTPIKYKVSNDTLDNYPLAELWRETSKKLDLDYKLILDILFYSISTCGYNETEKSWF
                     SSIYSNVLDSKKQNKIYTKLEKLKFNGLTRKLLRVLLNEFDHKEIFNYCLEIYYKLRN
                     LLTIEQCKKQRVEFTKNYYRQNEYCPALNAPILNYWLDKAHSFIYDDISFTKFFYTTF
                     SNYQLLEYKFPFYTSINVFARAHELGLMTEEQVNREMISGYNASNIIRTLTNPRWAGD
                     TLEKYPWLKDNLKTVTNRIIEIESRRGDMPTEVSNLANNIQYFEGINYFVNILVALGK
                     ETFQRGYSYGSNYSKKDVLSSLLKNCYPTKEDDSKKLKSLLKNTDITELRLIESAMYA
                     PQWVNIIEKYLNWKGLKSTIWYFHAHINEGFSAQKESEVALFSPISPRSFIDGAFDVD
                     WFLDSYKTIGEERFNILYKSAKYITSGGNSHRRAQLYADATLGKLDKDMLMKEIETNR
                     NQDKLRSFSLLPMKKGDLKEATFRYEFIQKFAKESKQFGAQRRESESKACTIALENLA
                     RSAGFGDVNRMMWALETEKLNEVAHLFKPKDIDGTSIWIDVDENGKASTKVQKGNKSL
                     KSIPKAIAKHPYVLELKSVQKDLKSQYSRAKQSLEQAMKNEIVFTVKEIANIYKNPVI
                     CPLIKNLVWVCDDNIGYVYNDKSKFFICNIEGETYKLTAKNKLKLAHPVHLFESGKWS
                     EFQHDLFSKQIVQPFKQVFREYYRINSDELSEKTISRRYAGHQIQPKKTVALLRNQLW
                     TVDYEEGLQKVFYKENIVAKMFALADWFSPADIEPPTIETVEFFDRKTYKSLELASIP
                     PILFSEVMRDIDLVVSVAHAGGVDPETSHSTVEMRIAIAKELLLLLKINNVNFEGSHA
                     LIAGNLGEYSVHMGSGIVHKKGMGALNILPIHSQSRGKIFLPFADEDPKTAEIMSKIL
                     LLAEDSKIKDPSILSNL"
     gene            complement(14039..14458)
                     /locus_tag="PC471_002683"
     CDS             complement(14039..14458)
                     /locus_tag="PC471_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2789800.1"
                     /translation="MQIYFRVKAVGKRKPMLELTPFDVSDNINCLKEAISEIVSKNVL
                     EYNEKIPEKSIINFLTNEEIESQADIGKVGFGSIYNENKQSVEKAIEVALQAFEDGIY
                     KVLINEDVIEKLDSSINLKSGDIFTFIRLTLLAGRMF"
BASE COUNT         5067 a         2082 c         2089 g         5390 t
ORIGIN      
        1 gaaatatatc tcatttagat aaaaaatttt agtaaaagta aaaaaaataa tatatttgtt
       61 aaaaaataaa ttgacaaaat attcagaaaa aaacttcttg acctaagaga gtaaaataaa
      121 taaaatctaa ggtatagaga tgaaatctat aaaattattt taagtataag ttaaaaatat
      181 agatgatgca tttaagattt ataaaaatta gagatgcata attattttag ggaggttttt
      241 agtatggaga aaaaagaaaa ggtagtagaa aaatcaaatg ttgaagtatg tagtcctgaa
      301 attgtcaaca gtgttgaaac attaagaatg agattagaag aaattagatt ggcacaaaaa
      361 gaatttgcaa catttacgca agaacaggta gataagattt tcttagctgc ttcaactgca
      421 gctaatcaac aaagaatacc tctagcaaaa atggcagtgg aagagactgg tatgggtatt
      481 gttgaagata aggttataaa aaaccatttt gcctcagaat atatctataa tgcttataaa
      541 gatactaaaa cttgtggtgt catagaaaaa gatgaagcat ttggttttac aagaattgct
      601 gagccagtag gtgttttatc agcagttata ccaacaacta atcctacatc aactgcaata
      661 ttcaaatccc taatagcact aaaaactaga aatggtataa ttttctctcc tcatccaaga
      721 gctaaaaact gtactataga agcagctaga gttgtacatg atgcagcagt taaggcagga
      781 gcaccaaaag gacttatagg ttgggtagat gttccttcga ttgaattaac taatgttgtc
      841 atggcagagg cagaccttat attagctact ggtggacctg gaatggttaa atctgcatac
      901 tcttcaggta aaccagctgt cggagttggg cctggaaatg tacctgcaat aatagatgag
      961 agtgcagata taaaaatggc tgtaagttca atcttagtat caaaatcatt cgataatggt
     1021 atgatatgtg cttccgagca agctgtaata gtaccagaaa aaatatatga agaagttaaa
     1081 aaagaattta aatatcgtgg agcacatttc ctaaataaag aagaaactga aaaagtagga
     1141 aaagtagtaa taattgatgg ttcattaaat gccagaatag ttggacaacc agcacatgta
     1201 atagccaaaa tggcggatgt agaagttcca aaaacagcta gaataataat tggtgaagta
     1261 gaatctgttg aattaaatga accttttgca catgaaaaac tttctcctgt acttgcaatg
     1321 tacaagtcta aaagttttga agatgcagtt gctaaagcag aaaaattagt tgcagatgga
     1381 ggctatggtc atacatcttc attatatgca gactctataa atcatccaga gagagtagaa
     1441 aagtttgtaa atgctatgaa gacttgtaga gtattagtta atactccatc atcacaaggt
     1501 ggtataggtg atttatataa ctttaaattg gcaccgtctc ttactcttgg ttgtggttct
     1561 tggggtggaa actcagtatc tgaaaacgtt ggagtaaaac atttgcttaa tatcaagaca
     1621 gtagctgaaa ggagagagaa tatgctttgg ttcagagcac cagaaaaggt ttatttcaaa
     1681 aaaggatgtt taggtgtagc agcaagagaa tttaaggatg ttatggataa aaagaaagca
     1741 tttatagtaa cagattcttt cctatataat aatggatata ctaagaaatt aactgattta
     1801 ttagatgaga tgggaattaa gcatactaca ttctttgatg tagctccaga cccaacatta
     1861 gcttgtgcaa gagaaggagc taaagctatg gctgatttcc aaccagattt aataatagct
     1921 gtaggtggag gaagtgcaat ggatgctggt aagataatgt gggtaatgta tgagcatcca
     1981 gaagtagatt tccaagactt agctatgaga tttatggata taagaaaaag agtatatgta
     2041 tttcctaaga tgggtgaaaa agcgtatttt gctgctatcc caacttcagc aggaactggt
     2101 tcagaagtaa ctccatttgc agttataact gaccaagatt caggtgtaaa gtacccatta
     2161 gcagattatg aattaatgcc aaacatggct ataattgatg ctgatatgat gatggagatg
     2221 ccaccaagat taacagcagc atcaggtgtt gatgctttaa cacatgcatt agaagcatat
     2281 gtatctatgt taagaactga accagctgac ggtctagcac tacaagcagg taaaataata
     2341 tttgaatatt tacctcgtgc atataaaaat ggaaagaatg ataaagaagc aagagaaaaa
     2401 atggcaatgg catctactat ggcaggaatg tcttttgcaa atgccttctt aggaatatgt
     2461 cactctttag cacataaatt aggtgcattc caccatgttc aacacggtgt tgcaaatgca
     2521 ttattaataa atgaagtaat caagttcaat tgtgcagaag ctccaaataa gatgggtgca
     2581 ttctcacaat acagatatcc tgattgcata caaagatatg ctgaatttgc ttcttttgca
     2641 ggtattaaag gaagtacaga ccaagaaaaa gtagataatc ttataaaagc aatagatgaa
     2701 ctaaaggcaa aagtaggtct tcctaagact ataaaagagg ctggagtaga agaatctaaa
     2761 ttcttagaaa gattggatgc tatggttgaa caagcttttg atgaccaatg tacaggtgct
     2821 aatcctagat acccattgat gagtgaatta aaagagatat atctaaaagt ttattatggt
     2881 aaataatatc tattgtgcaa cataggttct attgggtttg tatataaatt attagatttg
     2941 aatataaata gagttttatt agagacacgt agaagtaact ataaataata gattataatt
     3001 taaattctaa atggaaatga taaaaagtca gtaatttaat ggtaatttcg aatatgtgta
     3061 tttaatcaat taactttaaa attagtcgga aaattttttt attacatata attttaaagc
     3121 ataatatata aaaaacttta tgaaaaaatt ataaaaatat tcagaaaatt ctattgacta
     3181 atttatttat catgatataa taagtttaga aaagaaaaaa attggggcct attaaggccc
     3241 ccttactatt ttttgataga tttatttaaa attactttct cattttgatt ttttatattt
     3301 attatagagt cagtataatc cacttcaaag ttggcaaaag cagagaataa atctatatct
     3361 cttaacaaat gaattgtcat acattgattg ttcttattta gccttagaat caatgctctt
     3421 acaaatggaa attgtattct agaattaaat aagttagaac catcagtatc tatatctaag
     3481 ctacgataat taatatctat aaatatatct acatcttcat tttcttctct tattattctg
     3541 tagtcaaaat ccaagtttaa atttaaatta cctaaaatca ataacattta aatcctcttt
     3601 tctataaata gttttagagt gttaatttaa gctctccatc tattatagat ataattctat
     3661 ctgcttttct agcaatattt tcattatgtg ttattattat aagtgtttgg ttatattttt
     3721 ttatagaact ttttagtaaa tccatgactt cttctgtagt tttactgtcc aaatttcctg
     3781 ttggttcatc tgctaggatt atacttggtt ttgcagataa agcacgtgca atagcaactc
     3841 tttgctgttg accaccagat aattggttgg gaagatgatt tttcctttca ttaagaccca
     3901 ataaatttat aaggtcatca atatattctt tatctatttt tttattgtct aactttagtg
     3961 gtaattctat attttccttt acatttaata caggaattaa gttatactgt tgaaagacaa
     4021 atcctacctt ttgtcttcta tatcttgcta attctttatc tgaaagacaa catatatctt
     4081 taccatctac ctttatagta ccattacttg gtctttcaag accacctact aaatgtagaa
     4141 atgtactttt cccacttcca cttggtccaa ttatagcaac aaattcgcct ttatttattt
     4201 ttaaatttgc attctttagt gcatgtacag atgtttcctt ttttccataa atcttaccta
     4261 agttaatagt ttccaatata ggcataatta ttcctcctat tctgtaatgt ttatagcttc
     4321 aactatacta attttattta tctttcttga tggaatatat gtagctagaa tacctacaat
     4381 tatatttgct acaacaacta gtatatatat tttgtaatct attgaaaatc ccaatccata
     4441 tgatacaaag ccctgcatat aatacatttt aaattgtatt ataattcctg acacgatagt
     4501 aattatactt gagagtacac catataatat tccttcataa agtatcatat ttttaaagcc
     4561 tctctcagag ataccaatag ctcttaacat tccaaattca ctagttcttg aagttaagtt
     4621 ataacttatg ttgtttatta tgttaagtac actgattatg aacattattg ctactatacc
     4681 ataactataa atcatagttc tagatgtcat cttatcagaa ttttcttttt ctaccatcat
     4741 atctactgtt gtagttccag gaacttcact tccaatttta cctaaaaggt tatttatttt
     4801 tttcttgttt gcattttcat taaggtctgc ataaactaag ttatacttat ctacaccagt
     4861 taattttttt aggtattcgc tactcgttat tacatctaca ccatcatctc cagagtacat
     4921 accattatct gcaaaaggat aacttataat agctccaact ttaaattcat attctaagta
     4981 ttcataatta tcttttagac tttcataaag acctgtatcc atcccaccct ttggatattt
     5041 gacttttata gtatcaccta cttttatatc tacagctggt gttccttcac caggagtaaa
     5101 agtctttgtg tatttctgtg ttttagaaat ttctggtaca tatacaatag ctaaattttc
     5161 ttttttcatt tttttcaagt ttatatttcc acttactaag tatttttcta atgactttat
     5221 catctcatca ttataacctt ttagcttttg ctttagtgta tatttgccag tagtcttatt
     5281 ttttatgaac agaggattta gcttataata aggagttgaa tttatagctt cgtagtaatc
     5341 taatctattt attttattta cctctattct tgtgtttaag atactagcag ttttgacttt
     5401 gtcaattcca tctatatttt ttattttatc tatctgatta tcattaaacc cttttatatc
     5461 tttaaaagaa gtattgttac caatttcatt gagataaaca tttgatagaa taaaatctgc
     5521 attcatatat gtttgttctt gagtagattt gttttgcaca tctaaaaaag aatatttata
     5581 attgtctttt atgacgatta tacctcctat actcatagat aatacaatta taatgaaccc
     5641 ctttttattt ctaaagatgt ttttcgcaga tatagatttt gttactgaca tatttaaagt
     5701 taattttgat attaaataat taactttggt tacattttta tcagctccaa aagtttttct
     5761 gatagcatca ataggagaaa ttcttcttat ttttaagtat gtaaagaaac ttataattag
     5821 tattattagt attgtacatg caattgataa gagaattatt ttatctggaa taacaaaagg
     5881 tgtaacattt atatttcctt caaattgaat atttcctaca gacctattaa atatttgtgc
     5941 acctcccata cctatacata tacctatagg cattgctatt agagcaagta tgaaaagttc
     6001 ataaagcata agtttgaaaa tcctaaagtt ggtggaacca atagctctta agacgccata
     6061 ttcctgtact ctttggtata ttgatataac atagatacta tatattacaa taccagcaaa
     6121 tagactcata gcaattgcaa cgtatttact tgaattatct aaagtaccgt ttttcatagt
     6181 agattctata agcattgagt ttcttcctat acttttctca tctaacatag cattttttat
     6241 tatattgtct atgtttgtat ttatatcact gccttcatta aattcaatat aagttgtaaa
     6301 atcattcagt tctgattcat tgagattaga aaaaatttca cattgtccct tttttttctc
     6361 tataaatcta tcttcaagga ttccaacaac tttaaaagtt tctggatttt ctttttgata
     6421 taatttaaaa gtaaggtttt gatttatctc tggttttaag cccatactat ttaaaatcca
     6481 tttctcgact accacctcat tgctagcttt tggaaatcga ccttttataa gtttagaatg
     6541 actagtcaaa taatttttat ttgtatatag tatgtttata ggtaattttt cattaacatc
     6601 tgtagacgca tagtaagatg ttatacccag atttttaata tctttgctag attttatgga
     6661 ctttaattga tttttcttta tatttttaaa gtatacatgg tcagttccaa gctctctttt
     6721 caatctatct acatctgctt gctgagcact tcttgagaga gtaccaatac caacaacgag
     6781 tgatgtagca agaattatac tcacaacaat tgctaaagac ctagctttat aactttttat
     6841 ataccttagt actattttca aattatcttt catatttacc acttcctttc ttaactaaat
     6901 caattatata tttatctaaa atatatagca aagtaagtat tttcaaattt taagtagtta
     6961 attgtacaaa ttcatctgta tttttacaag tttaattaat atatatgtaa attttaactt
     7021 tttacataat ttttttaaaa tgatataatc cctatataaa ttgtagaggg gaggggaatc
     7081 atggataaca attttgtatt tcctatagta atactaactt tgtttatatt tagcaatctt
     7141 ataatgaata agtataaatt ttacaaagaa ttagacatat taaaaagagg catattaaca
     7201 ttttttatat cgtgttttgc attgatttta tttatggagg gcactaaata tatacaatta
     7261 aggttaggat atagtttttt acttgaagga gtattaaata ttatactttt tttggtttta
     7321 attattttaa gtttttatac aggctttaaa gttataagat tgataaataa taataatgaa
     7381 caggaatata aattacaaaa actagatagt aaaataaatc ataaggataa tataattatc
     7441 aaattaagaa gtcaaaaaca tgactatttg aaacatttgc aagtagtgta ttctctatta
     7501 aatactgggc tagatgaaga tgccaaagaa tatataatga atatatcaaa tagctttaaa
     7561 tcaaaaaatt caaggtatgg aaaagtatct tatttagatg ctatagcttc attaaaatac
     7621 gaggaatgtc ttgaaaagga tatatctttt gatgtctaca ttcaagattt tatggataat
     7681 ttagagattg aacctaatga tatgagttca gtactattaa acataataga taatgcaata
     7741 gagtctttgt ctactataaa aaaagaaaag aagtatataa gattacatgc atatgaagat
     7801 gaatttcagt atattatagc tataaaaaat aatggtccta aaatacataa tgttgaaagt
     7861 atattttgga atggatttag tactaaagaa ggacaagata ggggatatgg gctacattta
     7921 gttaaaaata tattagataa gtatgattat agtatacttg taaatagtga tgatttttta
     7981 actgaatttg taatcttaat cccaaaatat tattagttag gaggagaaaa gtctatgata
     8041 aaagttttga tttgtgatga tgataagatt gtaagagatg atatagcaaa tatagctaaa
     8101 gagtctggat atgtagatta tgttaaaaaa gttaaggatg gtatagaggc aattgaattt
     8161 ataaaaaatg agaaaataga tttattgatt attgacattg atatgccata taaaaatggg
     8221 attgattctg ctgaagaaat tctttctata gataaggata tacatatagt atttgtgacg
     8281 ggtttcgctg attatagtct tgaaagcttt aaagtgcatc ctatagattt tgtagttaaa
     8341 ccatttaaaa aagaaaaaat acttgattca attaacatag caatagacca tattaactcc
     8401 cataagattg ctaataataa ttttatagaa gacacccttt ttgtatataa aataaggaag
     8461 cagatacata tgattaattt tgatgatata gttatgtttg agaaaaattc aagagcaatt
     8521 aatctacaca ctaaagatga agatataata aagttttatg agaactttga tgatttaaaa
     8581 aaacgcctac ctcctaattt ctttatgacc catagacaat acatagtaaa tctaagattt
     8641 atacataagg ttatacctat aaataaatct tcattagaga taaggtttat aaattttcaa
     8701 gaaaatgcca tattaaacaa atctctagaa aaagattttt tatatagatt ttatagggtt
     8761 aaaagattct agtgaagata gaagaaaaat actttttgtg tgatataata agtaccgtaa
     8821 agataatgta taagagaagt ttatataaaa attatttgta attatgattt aatctatata
     8881 aattacagta tagtatatat tggactttct aattttttta agaaagtcca atatatttta
     8941 gagatagttt ttatacttat aaattactta agatactagg gtctttaatc ttactatctt
     9001 cagcaagtaa tagtatctta gacataatct cagcagtttt agggtcttca tcagcgaatg
     9061 gaaggaatat cttacctcta ctttgagaat gtataggaag tatattcaaa gcacccatcc
     9121 cttttttatg tacaatacca gaacccatat gcacagaata ctcacctaaa ttacctgcta
     9181 taagagcatg agagccttca aaattgacat tgttaatttt taataataat aaaagttctt
     9241 tagcaattgc tatacgcatc tctacagtag aatggctagt ttcagggtca acaccaccag
     9301 catgagcgac acttacaaca aggtctatat cacgcataac ttctgagaat agaattggag
     9361 gtatagaagc taattctaaa cttttataag tttttctatc aaaaaattct actgtttcaa
     9421 tagtaggagg ctctatatct gctggtgaaa accaatctgc aagtgcaaac atctttgcca
     9481 ctatattttc tttataaaat actttctgta atccctcttc ataatcgaca gtccaaagct
     9541 gatttctaag aagtgctaca gttttctttg gctgaatttg gtgaccagca tatctgcgag
     9601 atatagtttt ttcagataat tcatcactgt ttattctata atactcacgg aatacttgct
     9661 taaatggttg aacaatttgc ttactaaata aatcatgttg gaactcactc cacttaccag
     9721 attcaaacaa atgaactgga tgtgcaagtt ttaatttatt ttttgccgtc aacttataag
     9781 tttcaccttc tatattgcaa ataaagaact tagacttatc attataaaca tatccaatat
     9841 tatcatcaca tacccatact agatttttaa tcaaagggca gataactgga tttttataga
     9901 tattggcaat ttctttaact gtaaatacta tttcattttt catagcttgt tctaaactct
     9961 gttttgcacg agaatactga ctctttaaat ctttttggac agatttaagt tcaagtacat
    10021 atggatgttt agcaatagct tttggtatac tctttaaaga cttgttgcct ttttgaactt
    10081 tagtagatgc ttttccgttt tcatcaacat ctatccaaat tgaagtacca tcaatatctt
    10141 ttggtttaaa tagatgagca acttcattta gtttttcagt ctcaagagcc cacatcatac
    10201 gatttacatc tccaaatcca gcacttctag ctagattttc tagcgctata gtacaagctt
    10261 ttgattcact ttcacgacgt tgtgcaccaa attgcttgct ttctttagca aacttttgaa
    10321 taaattcata acggaaagtt gcttctttta ggtctccttt tttcatagga agtaaagaga
    10381 aacttctcaa tttatcttga tttctatttg tttcaatttc tttcatcaac atgtctttat
    10441 caagcttacc aagtgtagca tcagcataga gttgtgcacg tctatgagag ttaccaccac
    10501 tagttatata tttagctgac ttgtaaagta tattgaatct ttcttcccca atagttttat
    10561 atgaatctaa aaaccaatct acatcaaatg caccatcaat gaaactacgt ggtgaaatag
    10621 gggagaatag agcaacctct gattcttttt gtgcagagaa gccttcattt atatgtgcat
    10681 ggaagtacca aattgtactt ttcagaccct tccagtttaa atatttttca attatgttta
    10741 cccattgagg ggcatacata gcagattcta tcaatcttaa ctcagtaatg tcggtatttt
    10801 taagtaaact ttttaatttt tttgaatcat cttctttagt aggatagcaa tttttaagta
    10861 gagaacttaa aacatctttt tttgaataat tgctaccata tgaatatcct ctctggaaag
    10921 tttctttacc aagagcgacc aaaatattta caaaataatt tataccttca aagtattgta
    10981 tattattagc tagattagac acttctgttg gcatatcacc tctacgtgat tcaatttcta
    11041 taattcggtt tgtaacagtt tttaaattat ctttaagcca agggtacttt tctaatgtgt
    11101 cgccagccca tctaggattt gtcaatgttc ttattatatt tgatgcgttg tatccactta
    11161 tcatttctct attaacttgc tcttcagtca ttaatcctaa ttcatgtgca cgagcaaaca
    11221 catttatgct agtatagaaa ggaaacttat attctagcaa ctgataattt gaaaacgtag
    11281 tgtagaaaaa cttagtaaaa gaaatatcat cataaataaa cgaatgtgct ttatcaagcc
    11341 aatagtttaa aataggggca tttaaagcag ggcagtattc attttgtctg taatagtttt
    11401 tagtaaattc aactctttgt tttttacatt gttctatagt caataagttt ctaagtttat
    11461 agtatatttc taaacaatag ttaaatattt ctttatggtc aaactcattt agtaaaactc
    11521 ttaaaagttt tcttgtaagt ccattaaatt taagtttttc aagttttgta tatattttat
    11581 tttgtttttt agagtcaagg acatttgaat atatagatga aaaccaactt ttttctgttt
    11641 cattatatcc acatgtactt atactataaa ataagatatc aagtattaat ttgtaatcta
    11701 agtcaagttt tttactagtt tctctccaaa gctctgctaa tggataatta tcaagagtat
    11761 cattagaaac tttatattta ataggagtca agttataagt agaataacct aaaagtactt
    11821 tgtctacatt acctgaccat ctttcaactt catattcata atctgcattt tcagtaataa
    11881 gttcatcaaa tttttcataa acttttatta ttttatttat atccgtatcg agaatttttt
    11941 ttgcttcttt ttttgttgct acagaaacag aatcctcacg agtagataaa cttatatttg
    12001 agataatttt attaagcttt gatgagctat tagaatcatc tttaaaatca aattgttctg
    12061 gaatttgaac tttgttttca gggttatata ttccaaaacc attttctttg cagtaatcat
    12121 tttttgcttt acctaacaaa ttatcaatta gtatttgaac ttgagatgta ttctttttag
    12181 atttatcaat atcctctata agtaaaataa gcttattata tagttcttgt ctagcagtat
    12241 tgtcttttaa taagtttaca atttcaagac ctccaaggca tttttgagaa tttgaagatt
    12301 ttatcagtga agaagcaata ttttccaaat ttgaatcatc ttgttcaagt aaaattttta
    12361 ttactgtttg tctaatagag cttgtcttta aagacaatag attggttatc ttatctaatt
    12421 ctacatctgt tagctcaagc tttctaagta gactcaatac cgtttctcta ttggaaacac
    12481 tcttatcact taaacattcc aataaaaatt cacgttgtct atctgtcttt ggttcattta
    12541 caaaattgac tataagtgaa gctcttaaat ctgaactcat aagatgacaa ttttccataa
    12601 gcacatctat aaattgagca ttcatatcgt atgctgttaa agtcatcata catttaagta
    12661 tttcatgaga agaaatttca atccaagtcc atggaaatac acttcctgaa aatgattttg
    12721 tctttgaatt taaaatttct aaagtatgtt ttaaactttc aaattgttct tttctagcat
    12781 caaagtcttt gtcaatagga gtgtttgaca agtcaaaatt agtttctctg ttttgccaac
    12841 tatagtagta gccaacagaa ccttctacat agaaatttgt aattacccat gctagtatct
    12901 ctaaatcatc acccttaatg tttttagatg ctatagaatg tttaaaatat gtactttctg
    12961 tatgcgataa aaagtaaaga gctacaagtt ttttatattt ttcctgacaa gaaagtaagt
    13021 tttcaataag agagtaagag tcatatatgt tgtaagttgc agtagcccat aaggcaaaat
    13081 aaatttcaag attatcattg ctattaattg attctatacg gaacttttca tcagtcaatg
    13141 atttatatga aagttctaaa catttacgta ttattccagg tttttggtct gaaaaaccaa
    13201 gtcctgtcca tgtatccaag gctcttataa cagaactaaa tcgctctaaa ttgttgtcca
    13261 aaatagtttt taacatgtat attgatgcag aaattgtacc ctcatctaaa ctctctacaa
    13321 tttgctgtct aagtccttct tgttgtttgg ctgccaaaag aagtttagaa agcatttcat
    13381 agcatttatc actatgactc ttcatgatac caattatcat ttgtcttgta agtagagcag
    13441 tttgattttc tccaaatatt acttcttcta aagcacttat tactttttga tttcctttgt
    13501 ctatttctag tgctatttta tcactcatta tagagtcata gtacaagacc ttatttggaa
    13561 gagtaagata atctataact gaaaaatcat aataaattaa atcaacatag tttgaaataa
    13621 accttataat acctctaaaa taatccacaa gtttatttga gcgataccct ttacgataat
    13681 atcctcctga atatgtataa tccatctttc ttttaattaa ttggcaaagt tcttctactt
    13741 gttcagaagt aaatattaat tctaatgcat tttttaaagg tccagaaagg agctcgtcca
    13801 tatttttaat ttcgtagtag tggcaattgt agtatttctt tatcatattt aagaatttgt
    13861 cttcaggttc tctataatag tatgaactat tttctatgca gtcccaaaga gactttgcta
    13921 agtcgtagta ctgttttgaa gaagatttag taaaattatt tttaaaattt tcaattattt
    13981 taggattttt ttctactcta gtacgttcta acatttatgt acctccaaat tatttatttt
    14041 aaaacattct tccagcaagc aaggtaagtc ttataaaagt aaatatatct ccacttttca
    14101 gatttataga ggaatcgagt ttttcaataa catcctcatt tataagcact ttgtagattc
    14161 catcttcaaa tgcctgtaac gctacctcta ttgccttttc tacagattgt ttattctcat
    14221 tgtaaataga gccaaatcca acttttccaa tatcagcttg agattcaatt tcttcattag
    14281 ttaagaagtt tattattgat ttttctggga ttttctcatt atattcaaga acatttttag
    14341 atacaatttc agagatagct tcttttaggc aattgatgtt gtcagataca tcaaaagggg
    14401 taagttctag cataggtttg cgtttgccaa cagccttaac tctaaaataa atttgcataa
    14461 aatcacctcg caaaatatta attatgtatt tagaaattta aaaatttatc tatttgttat
    14521 attatatcat tttgtgaatt attaatgtga attatggaat tattttacaa aaaatagaaa
    14581 aaaagctatc ttaaagtcta aaaaagcaat aaaagataac ttttaact
//
LOCUS       DAMJYR010000091        14502 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.094, whole genome shotgun sequence.
ACCESSION   DAMJYR010000091 DAMJYR010000000
VERSION     DAMJYR010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14502)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14502)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14502
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.094"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(27..278)
                     /locus_tag="PC471_002684"
     CDS             complement(27..278)
                     /locus_tag="PC471_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2789801.1"
                     /translation="MNRYTKIINMMGSYYTKDFEKEKKNVIKVREVKEDTVRKFFLQG
                     DCEVLVVFEDTGKEILIDDFSPEEDIKKYLGAKFINKKR"
     gene            367..1098
                     /locus_tag="PC471_002685"
     CDS             367..1098
                     /locus_tag="PC471_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HDF2789802.1"
                     /translation="MTSIKNKDILNCIDYSIKNKLFDKLNDVYKSLPVGNCSGCGNCC
                     MESVGINLIEFLNIFCYLEDRVDLKKSCISKILDYYFEEYTRKNPCPFKDTNNRCLIY
                     EVRPLNCRMFGHWKKEDYNKNLDNVIEKNNNYRELMKKQYGFEINDEVVNYRIDYCDR
                     FIPNKDYLSKSKRLSFFDELMILDSNIYSSGNIDIDFRDRGIVEYFIESLLYRNLAYN
                     VKIRISKEYKIRKRTIDRIKRIVLV"
     gene            1157..2068
                     /locus_tag="PC471_002686"
     CDS             1157..2068
                     /locus_tag="PC471_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDF2789803.1"
                     /translation="MNLTKVKGNTFFIKGGTNTGIYVFEDNTALMIDPGLCGSRPRRV
                     NKVLDENNIQVRYIINTHGHDDHYGACNQFKEYYKDVCIMSSEYDKLYIENPELFSKY
                     IVGGKSNVFMDNILKNKMLDDIKIDKSIEAGKIELNNEMFDIIEFQGHTPGSIAVLTK
                     DNVIFVGDLLIGDEILSKYDFLFLYDIQEQINSLEKLKNIDFEYLVLGHGKQVISKED
                     SYKLIEKHLNALKKYLYQIRELLVEPKSLEILLKNIINNNNLSNNYKEYHFFKSSLVS
                     VISYLVDLEEIGYILENGELLYYTKTK"
     gene            2119..2931
                     /locus_tag="PC471_002687"
     CDS             2119..2931
                     /locus_tag="PC471_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HDF2789804.1"
                     /translation="MEYEKWNEILAPYEHAVEELKIKFKNIRKEYLAKGEYSPIEFVT
                     GRTKKISSIISKLKRINAKDIETEIDDIAGIRIMCQFVEDIYAIVDLIKVRNDMTIIG
                     EKDYITNYKDSGYRSYHVIIKYPINSIAGSKEIICEIQIRTLAMNFWATIEHSLKYKY
                     DHYIPETLAERLRRASDAAFLLDQEMSEIREDIMKAQAMYQMKSIAIRDTLNRIQELY
                     DLGDTHKAMQYQRKLDRTETDKNITEILELKQEIDILLEQYKDTKVDDKVVQ"
     gene            3040..3732
                     /locus_tag="PC471_002688"
     CDS             3040..3732
                     /locus_tag="PC471_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HDF2789805.1"
                     /translation="MSLYAIGDLHFSTSVNKPMNIFGSNWDGHEKKIIDNWKEVVKEE
                     DMVLVLGDTSWGINLSEAKKDLDIISKLPGQKILIKGNHDYWWTTVTSLNKLYEDMRF
                     IQTNFYEYKDYAICGGRGWICPNDVKFDETDEKVYKREEHRLRLSLESARKSGHSKII
                     VITHYPPTNDKLEESLFTKLFEEYNVEKVIYGHLHGKESFKMGLKGIRNGVEYTLASC
                     DYTEFNLIKVHD"
     gene            3819..4760
                     /locus_tag="PC471_002689"
     CDS             3819..4760
                     /locus_tag="PC471_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2789806.1"
                     /translation="MRWILVFIFLYLIPLVVIFRNYKNFKRSCIYSSIYVVLATTIMI
                     SNIYMSGLNKIKESMYYHNYISDETYDEKYVSNFKEKESTDLKQDNKKNEEISNNKNL
                     NNKQDRNIQNKESNEEGSNDIKEKNNSDSNVSVNNKSEKQEDNTKENNKYTSKEKNSE
                     DIKKIDMESIGIFKKEIYNIERAALVPMRECIPYTKDIAKNITKLSSIKKDVTNARNK
                     CKEVVKEYENMQIPELSKEKYTETLSNARDDVKKAYELREKSMESALKLINTKSPKYI
                     GKITEYLDLSDKQIESFKNRLNDLKEEINNSETNNIN"
     gene            4797..5954
                     /locus_tag="PC471_002690"
     CDS             4797..5954
                     /locus_tag="PC471_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HDF2789807.1"
                     /translation="MNKQSIGKLNMFFMGITQRNLDNRDFFIELEVIFKSGRKEFKGI
                     AIEEDNKYKFNFKGKSDLYTFNELLKNISKEAENYDGLVFKYVERGTIVVIEGDNKKV
                     NVKYLDNKEEVPKIDEFTASQIKNRDYYVKVGQANALLKEIGVLTKDGKIKNDKIRKY
                     NQIDHFVELIDSILKEIKDKDCITILDCACGKSYLSFVLNFYIKEVLKKNCYFIGIDY
                     SDVVIEASKNMAKNLGYKNMSFIKEDLTNYTPNRDVDLVISLHACDTATDMAIGLGIR
                     AKSEAIVVVPCCHKELLGQYRYEAMEPILKHGVFKARFADLITDGLRTLLLEGNGYDT
                     SVVEYISPLDTPKNLMIRAIKTKTNNDKALKEYKELKSQFGVEPTLEKLIY"
     gene            6106..6666
                     /locus_tag="PC471_002691"
     CDS             6106..6666
                     /locus_tag="PC471_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HDF2789808.1"
                     /translation="MNKLNICIDIDGTITSPYHFLPYLNDIFNKNITEAECITHNWEE
                     LYGSGIRDVYAEFNNKYIHSYDEAKIVEGATEVISTLSKGHNLSFVTARHECLTDVTK
                     NWLSRQGFSDIDVYLLGSDYKVEKARELSCDIFIEDNPLNSVQLADDGVKVILLDTNY
                     NKDVKHQNIVRVSNWIDIEKIIKQGI"
     gene            6694..7185
                     /locus_tag="PC471_002692"
     CDS             6694..7185
                     /locus_tag="PC471_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2789809.1"
                     /translation="MIDLKIENRKGKIHVKSSEIKDILELRPDFEYVQDISNTINQEN
                     ILVFDCQLTGNSFDMDDLDIEEILEELGEEIDESYFSVLFEDVRAYLKDATDEIEADL
                     QDNYLFDNLRCYFDIYNINQEFTDFKFVFLVSFKDIKISSLANLAKIVSKRQLIGASK
                     FYS"
     gene            7414..7965
                     /locus_tag="PC471_002693"
     CDS             7414..7965
                     /locus_tag="PC471_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF2789810.1"
                     /translation="MQSLNEIIAENLKKIRKEKHLSLDKIAQLSGVSKSMLSQIEKCE
                     VNPTISTLKKITNGLKISFTSLMERQESDIELIQKSDIDHFIEDNGKYISYPIFPFDS
                     KRRFEIFMIEIEEGGNLDSNAELPGTQEFITVFSGEVTIKINGEDYIVSSGNSIRFKA
                     DVSHIYRNSGKGIAKMSMVVYYI"
     gene            8051..8983
                     /locus_tag="PC471_002694"
     CDS             8051..8983
                     /locus_tag="PC471_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HDF2789811.1"
                     /translation="MNNKKAVFFFCSDLKKDPVASNVIKCCEEIMDLNQTDIVIDDNF
                     VLEFKDKNNNLFHFVKTKDVISHNYKYYLPILNRHFRDYDFAGVVNWHEGENAPEHIL
                     TAHTIGDVPTGEFGNSNPRYFKNLINAIEDIKNENLLDEFTTLTEATHWSGTTYGEES
                     KLITEYSVPMLDIEIGSSSDSFNNSIAIQVLAKSLIRVFDCDEPLKTLLCVGGVHFEK
                     SFSDIIKNKEYNISIGHVLPNQWIVSGMYDDESGFEKLEKCINSIEGGIDCIVFHDKL
                     KGTYKEQCRKLGEKLNVPVFKHKILKNPKDLPIW"
     gene            9038..9901
                     /locus_tag="PC471_002695"
     CDS             9038..9901
                     /locus_tag="PC471_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HDF2789812.1"
                     /translation="MILSYFALLIRVVLLAFERVVVRLLGDEEGDIYKNIASSFLFFF
                     IGGVCLLPFSIMDRVSDWSFLIPCYISSLVYSIGSVAYVTSLATGEVSLVTPINSLNS
                     LFLLLLSVIFLGEGLSLAKVAGIIIMIGGVFILKKVSSFLKNVSVIFNNLPCRLMFLY
                     IFLQSVGRVLDKAFFVQVSPILYSTILYFFVGLNLFIFLAFKKKQKIICEIFTNKKGL
                     SILSGAINGYSYLALLIALNNLELSIAEPFSQVSMIITLILAHFIFKENIKEKIPGSI
                     LILIGGWLLLL"
     gene            10148..12010
                     /locus_tag="PC471_002696"
     CDS             10148..12010
                     /locus_tag="PC471_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDF2789813.1"
                     /translation="MNYKKTAIDIIEQVGGSKNILALEHCSTRLRFKLIDRSKVNQDA
                     LKKVQGVMSIVNGAQFQVVIGNSVVDVYDEILKVCPLDTNDSTVKTEEKINLGTRFIE
                     FIINIFQPLIFAIAGAGILKSLLMLLAMFNILKSDSTIYTLLVAISDATFYFLPLMVA
                     VTTANVLKCNRLVAIAAVGYLLLPATTTALSEGVELFGFTIPNIAYNAQVFPAILCVS
                     FLATMEKIFNKYSPKPIRTFFVPMMSLAITVPITLTILGPLGYNVGTIFTTIILFLYN
                     KMGFLVVGLLAAFLPFMVATGMHKALIPYSINTIGKLGYEALYMTSSLAHNISESGAC
                     FAVALRTKDETLKQTALSAGISALMGITEPALYGITLQHKRAMLGVVFSSAISGTFLG
                     LFAVKGFVIVGPGLASMSMFIDPNNGNNLIFASIGFVIAILGSFIITLIIWKEDTNTV
                     ESSIGDVVEEELEEEKEVNILVTPVEGKVIDLSKVNDELFASKTLGDGVAIIPTNGNL
                     YAPCDSEVVMLFETKHAIGLRTKNGAEILIHIGINTVSMNGDGFKTFVKTGDNVKEGD
                     LLIQFDLDKISHANLDSTVMIVNNNGADYAYKVLNQSYGNVKKGSILFDVKGGI"
     gene            12013..13479
                     /locus_tag="PC471_002697"
     CDS             12013..13479
                     /locus_tag="PC471_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HDF2789814.1"
                     /translation="MRKFPEGFLWGGATAANQFEGGWNLGGKGWSVSDVAKAHFDADV
                     KDYKSNNEITTKDIEEGLAHPEDEVNYPKRHGSDFYHHYKEDIALMAEMGFKTYRMSI
                     AWSRIFPNGDDKEPNEEGLQFYDDVFDELISYGIEPLVTMSHYEPPLNIVLNYDGWYS
                     RQVINMFVRYVETICERYKNKVKYWLTFNEVDSMIRHPYTTGGLVRDRFKDKNFEEVI
                     FQAMHHQFVASAIATKICHEIIPNSKVGCMLTKLTYYPYTCRPEDVLATQQKMRSIYA
                     YSDTQVFGEYPVYLLSYFKNNNIQIVKEEHDDEIMKKYPVDFISFSYYMSSCEAADTT
                     GLDITPGNTLLAVKNPYLEMSEWGWQIDSIGLRISLIELYDRYRKPLFIVENGLGAKD
                     ILTEDKKVHDQYRINYLKEHFKCMLDAIIEDGVELWGYTSWGCIDLVSESTKQMSKRY
                     GYIYVDADDYGKGTYNRYKKDSFYWYKKVIENNSIDFK"
     gene            13503..14339
                     /locus_tag="PC471_002698"
     CDS             13503..14339
                     /locus_tag="PC471_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDF2789815.1"
                     /translation="MNYIIKKVLNSSVVLVHDAKYNEFILLGKGIGYGKKTGEYICGS
                     NDNQMFVPVENTKSKQFLELMDDIPVDILKITQEIIVEAEKLLNSSFNKNLYLILADH
                     FNFAIERMRKGIKITNRVFWEIKNYYPNEFKVGMVAIYMVEERLGIQLPEEEAANIAF
                     HFANAMASDGNSYDTIKYAKVIGEIINIFVFSLNRTLDKKSMHYMRFITHIKFFVERF
                     FSDSMLSSGDDLLFNQMKRSNPKEMLIALKVRDFLEKKYGKKLTNEEIAFLVVHIARV
                     KQ"
BASE COUNT         5715 a         1414 c         2568 g         4805 t
ORIGIN      
        1 tttaatttta gaattatttt tgataactat ctttttttat ttataaattt tgctcctaaa
       61 tattttttga tatcttcttc aggagaaaag tcatcaataa gtatttcttt cccagtgtct
      121 tcaaatacta caagaacttc acagtcacct tgtaagaaaa attttctaac tgtatcttct
      181 ttaacttctc taacttttat tacattcttt ttttcttttt caaagtcttt tgtgtaataa
      241 cttcccatca tattaattat ttttgtatat ctattcataa aatacctcca tatgcgatta
      301 aatttaaaaa atatgttata ataattgttt gtattaactt aataattata acaaggagta
      361 ataataatga ctagtataaa aaataaagat attttaaatt gtatagatta ttccattaaa
      421 aataagctct ttgacaaact aaatgatgta tataagtcct tgccagtagg aaattgttct
      481 ggatgtggaa attgttgtat ggagtcagta ggtataaatt taattgagtt cttgaatata
      541 ttttgttatc ttgaagatag agttgattta aaaaaaagct gtataagtaa aatattagat
      601 tattattttg aggagtatac aagaaagaac ccatgtccat ttaaagatac taataataga
      661 tgtttaatat atgaagttag accattaaat tgtaggatgt ttggacattg gaaaaaagag
      721 gattataata aaaatttaga taatgttatt gagaaaaata ataactatag agagttaatg
      781 aaaaagcaat atggatttga aataaatgat gaagtagtaa attacaggat agattattgt
      841 gatagattta ttccaaataa agattattta agtaaatcta agagattaag cttttttgat
      901 gagcttatga tattagactc aaatatttat tcaagtggaa atatagatat agactttagg
      961 gatagaggta tagttgaata ttttatagag tcattacttt atagaaattt agcatataat
     1021 gtgaaaataa ggatatctaa agaatacaaa ataagaaaaa gaactataga tagaataaaa
     1081 aggattgttc ttgtatagtt ttatattagt aagtaataaa ttgtatcata aatattgtca
     1141 aaatgaggtg aagaaggtga atcttacaaa agtaaaagga aatacctttt ttataaaagg
     1201 tggcacgaat acaggaatat atgtatttga agataacaca gcacttatga tagacccagg
     1261 gttatgtggt tcaagaccta ggagagtaaa taaagtatta gatgaaaata atatacaggt
     1321 tagatatatt ataaatactc atggtcatga tgaccactat ggagcttgta atcaatttaa
     1381 agaatattat aaagatgtgt gtataatgtc atctgaatac gataaattat atatagaaaa
     1441 tccagaattg ttttcaaagt atattgttgg gggaaagagc aatgtattta tggataatat
     1501 cttaaaaaat aaaatgttgg atgatataaa aatagataaa tctatagaag ctggaaaaat
     1561 tgaattaaac aatgaaatgt ttgatataat tgaatttcag ggtcatacac ctggtagtat
     1621 agctgttttg acgaaggata atgtgatttt tgttggcgat ttattgatag gagatgaaat
     1681 tctatcaaaa tacgatttct tgtttctata tgatatacaa gaacaaataa attcattaga
     1741 aaaattaaaa aatatagatt ttgagtactt agttttaggg catggcaagc aagttatatc
     1801 gaaagaagat tcttataaat taatagaaaa acatttgaat gctttaaaaa aatatttata
     1861 tcaaataaga gagttgcttg tagaaccgaa atctttagaa attttattaa aaaacattat
     1921 aaataacaat aatttaagca ataattataa agagtatcat ttttttaaat cttcgttagt
     1981 atctgtcata agttacttag ttgatttaga agaaataggg tatatattgg aaaatggaga
     2041 attgctttac tatacaaaaa caaaataaat tatgataaaa taaagaaggt cataaaaaac
     2101 aaataaaggg tgagcaatat ggaatacgaa aaatggaatg agatactagc accttatgaa
     2161 catgcagtag aggaattgaa aataaaattt aaaaatataa gaaaagaata tttagcaaaa
     2221 ggtgaatatt ctcccataga gtttgtcaca ggtagaacaa aaaaaatatc ttccataata
     2281 tctaagttaa aaaggataaa tgcaaaagat atagaaactg aaattgatga tattgctggt
     2341 ataagaataa tgtgccagtt tgttgaggat atatatgcta tagttgattt gataaaagta
     2401 agaaatgata tgactataat aggagaaaaa gattatatta caaattacaa agatagtggt
     2461 tatagaagct atcatgttat aataaaatac cctataaact ccattgcggg ttcaaaggaa
     2521 attatatgtg aaatacaaat aagaactctt gcaatgaatt tctgggctac aatagaacat
     2581 tcattaaaat ataaatatga ccattacata cctgagactc ttgctgaaag gcttagaaga
     2641 gcatctgatg ctgcctttct tttagaccaa gaaatgagtg aaataagaga ggacataatg
     2701 aaagctcagg ctatgtacca aatgaagtct atagctatta gagatacttt aaatagaata
     2761 caagagttat atgacttggg tgatacacat aaagctatgc agtaccaaag aaaacttgat
     2821 agaactgaaa cagataaaaa tataactgaa attttggaat taaaacaaga aatagatata
     2881 ttattagagc aatataagga tactaaggta gatgataaag tagttcagta aaaactactt
     2941 gtagaaaatt tgctaaatat taatggggct gcctcttaat ggaaaataat ttctaaaaag
     3001 agacagccta tttagtgtaa ttaagaaagg aatgatatta tgagtttgta tgctataggt
     3061 gatttacatt tttcaacatc agtcaataag ccaatgaata tctttggaag taattgggat
     3121 ggtcatgaaa agaaaattat agataattgg aaagaagttg ttaaggaaga agatatggtc
     3181 ttagtactag gagatacatc ttggggaatt aatttatccg aagctaaaaa agatttagat
     3241 ataataagca aacttccagg tcagaaaata cttataaagg gaaatcatga ttactggtgg
     3301 acaaccgtta caagtttaaa taagctatat gaagacatgc gttttattca gacaaatttt
     3361 tatgaatata aagattatgc aatttgtgga ggtaggggct ggatatgtcc taatgatgtt
     3421 aaatttgatg agactgatga aaaagtttat aaaagagaag aacatagact tagactatca
     3481 ttagaatctg ctcgtaaaag tggtcactca aaaataattg tgattactca ttatcctcct
     3541 acgaatgata aattggaaga atctttattt acaaagttat ttgaagaata taatgttgaa
     3601 aaggtgattt atggtcactt gcatggtaaa gaatctttta aaatgggatt gaaggggatt
     3661 cgaaatggag tagaatatac attagcatct tgtgactata cagaatttaa tttaataaaa
     3721 gttcacgatt aaaaaacata aattggtaaa tactataaga tacatgatta aaaataaata
     3781 tttgagggat ttcggttttg agaaaggaga cacgacttat gaggtggata ttagttttta
     3841 tatttttata tcttattcct ttagtagtga tattcagaaa ttacaagaat tttaagaggt
     3901 cttgtatcta cagtagtatt tatgtggtat tagctacaac aataatgata agtaatattt
     3961 atatgagtgg acttaataaa ataaaagagt ctatgtatta tcacaattat atttctgatg
     4021 agacatatga tgaaaagtat gtatcaaatt ttaaggaaaa agaaagtact gatttaaaac
     4081 aggataataa aaagaatgaa gaaatatcaa ataataaaaa tttaaacaac aaacaagata
     4141 gaaatataca aaataaagaa agtaatgaag aaggaagtaa tgatataaaa gaaaagaata
     4201 attctgatag taatgtttca gtgaataata aatcagaaaa acaagaagat aatacaaaag
     4261 aaaataataa atatacaagt aaagaaaaaa atagtgaaga tataaaaaaa atagatatgg
     4321 aaagtatagg aatttttaag aaagaaattt ataatataga aagagcagct ttagttccaa
     4381 tgagagaatg tataccttac actaaggata tagcaaaaaa tataactaaa ttatctagta
     4441 taaaaaaaga tgttacaaat gccagaaaca agtgtaaaga agttgtaaaa gaatatgaaa
     4501 atatgcagat tccagaactt tctaaggaga agtatacaga aacattgagt aatgctagag
     4561 atgacgtaaa gaaagcatat gagcttagag aaaaatctat ggaaagtgct ttgaaattaa
     4621 taaatactaa aagccctaaa tatataggga agataacaga atatcttgat ttatcagata
     4681 agcagataga aagttttaaa aatagattga acgatttaaa agaagaaata aataattcag
     4741 aaacaaataa tattaattaa taagatttaa agatataagt taaaagggag attatgatga
     4801 ataaacagag tataggaaaa ctaaatatgt tttttatggg gattactcaa agaaaccttg
     4861 ataataggga tttttttatt gagttagagg tgatttttaa atctggaaga aaagagttta
     4921 aaggaattgc aatagaggaa gataataaat ataaatttaa ttttaaagga aaatcagatt
     4981 tatatacgtt taatgagttg cttaagaata tttctaaaga ggcagaaaat tatgatggat
     5041 tagtatttaa atatgttgag cgaggaacta ttgttgtaat tgaaggagac aataagaagg
     5101 taaatgtaaa atatttagat aataaggaag aagtacctaa aatagatgaa tttacggcat
     5161 ctcaaataaa aaacagagat tattatgtaa aagtaggaca agcaaatgca cttttaaaag
     5221 aaattggagt acttacaaaa gatggtaaga taaaaaatga taaaataaga aaatacaatc
     5281 agatagacca ttttgttgaa ttgatagata gtattttaaa agaaataaag gataaagact
     5341 gtataactat cttggattgt gcttgtggaa aatcttactt aagctttgtc ttaaattttt
     5401 atataaaaga agtattgaag aaaaactgtt attttatagg aatagattat tcagatgtag
     5461 ttatagaagc atcaaaaaat atggctaaaa acttgggtta taaaaatatg tcatttataa
     5521 aagaggattt gactaattat acacctaata gggatgttga ccttgtaata agtctacatg
     5581 cttgtgatac agctacagat atggcaatag gtctaggaat tagagcaaaa tctgaagcta
     5641 tagtagtagt accatgttgt cataaggagt tattagggca atatagatat gaagctatgg
     5701 aaccaatatt aaaacatggt gtatttaaag ctagatttgc agatttaata actgatggtt
     5761 taagaacatt gttattggag ggcaatggat atgatacttc tgtagtagaa tacatatctc
     5821 cacttgatac accaaaaaat ttaatgataa gagcgataaa aactaaaacg aataatgaca
     5881 aagcattaaa agaatataaa gaactgaaat cacaatttgg agtggaacca accttagaaa
     5941 aacttatata ttagttaaaa atgtaaaatt tacgaaaagc ttgcttaaat atgcaagctt
     6001 tttggtatta tataggtata gtcatatcca gagttttgat ttataattaa agctgtgcta
     6061 taatatttga ttaatcaaat caataaatgt tacatggagg taaaaatgaa caaattgaat
     6121 atatgtattg atatagatgg aacaataaca agcccttatc attttcttcc atacctaaat
     6181 gatatcttta ataaaaatat aactgaagca gagtgtataa cacataattg ggaagaattg
     6241 tatggttcgg gaataagaga tgtgtatgcg gagtttaata ataagtatat acactcatat
     6301 gatgaagcaa agattgtaga gggtgctaca gaggtaatta gcacattatc taaaggacat
     6361 aatttatctt ttgtaacggc tagacatgag tgtttaactg atgtaactaa aaattggcta
     6421 tcgagacaag gattttcaga tatagatgtt tacttattag gaagtgatta taaagttgaa
     6481 aaggcaagag aactgagttg tgacatattt atagaagata atcctttaaa ctcagtgcaa
     6541 ctagctgatg atggtgttaa agtaatactt ctggatacta actataataa agatgttaaa
     6601 catcaaaata tagttagagt tagtaattgg atagatattg aaaagattat aaaacaaggt
     6661 atttaaagat ttgtagattg aaagggggag catatgatag atttaaaaat tgaaaataga
     6721 aaaggaaaaa tccatgtaaa gagcagtgaa ataaaagaca ttttagaatt acgtccagac
     6781 ttcgaatatg tacaagatat atcaaatact ataaatcaag aaaatatatt ggtatttgat
     6841 tgccagttga caggaaattc ttttgatatg gatgatttag atatagaaga aatattggaa
     6901 gagttaggtg aagaaataga tgagtcttat ttcagtgttt tatttgaaga tgtaagagcg
     6961 tatttaaaag atgctactga tgagatagaa gcagacttac aggataatta cttatttgat
     7021 aatttgagat gttattttga tatatacaat ataaatcaag aatttacaga ttttaagttt
     7081 gtatttttag tttcttttaa agatataaaa atttcttcat tagcaaacct agctaagatt
     7141 gtttctaaga gacagcttat aggtgcatct aagttttata gttaaaatga aatattatta
     7201 taataaaagc tagtaacctt gtaaaaatat aggttactag ttttttattt attcattttc
     7261 acaaaatttc agaaaactat ttacattcta aaaaaagtaa tgtatattaa acataatgta
     7321 cattataatg gataaaaagt ttctattaaa ttactaagtt tatttaataa tcaaaataat
     7381 ttatcttata ttagtatgta ttagggggta tttatgcaaa gtttaaatga aataattgct
     7441 gaaaatttaa aaaagataag gaaagaaaaa catcttagtt tagacaagat tgcacaactt
     7501 agtggtgtaa gtaagagtat gctatctcaa attgaaaagt gtgaggtaaa tcctactata
     7561 tctactttga agaaaataac taatgggcta aaaatttcat ttacatcatt gatggagagg
     7621 caggaaagtg acatagaatt gattcaaaaa agtgatatag accattttat tgaagataat
     7681 ggaaagtata taagttatcc aatatttcca tttgactcca aaagaagatt tgagatattt
     7741 atgattgaaa tagaagaagg tggaaattta gattcaaatg ctgaacttcc aggaacacaa
     7801 gagtttataa ctgtttttag tggcgaagta accattaaaa taaatggaga agattacatt
     7861 gtatctagtg gaaattctat aagatttaaa gctgatgtat cacatatata tagaaactca
     7921 ggcaaaggta tagctaaaat gagtatggta gtatattata tataaataat ttaatggaat
     7981 tagagtgata tttagatgat tttaggtatg aagatatatt atatttaagt tataggagtg
     8041 tgatttatgg atgaataata aaaaagcagt ttttttcttt tgcagtgatt taaagaaaga
     8101 cccagtagcc agtaatgtga taaaatgttg tgaagaaata atggatttaa atcaaactga
     8161 tattgttatt gatgataatt ttgtattaga gtttaaggat aaaaacaata acttatttca
     8221 ttttgtaaaa acaaaagatg taataagtca taattataaa tattatcttc caattttaaa
     8281 tagacatttt agagattatg attttgcagg agttgtaaat tggcatgagg gagaaaatgc
     8341 tccagaacat atcctaacag ctcatacaat tggagatgtt ccaacaggag aatttggaaa
     8401 ttcaaatcca agatacttta aaaatctaat caatgctatt gaggatataa aaaatgaaaa
     8461 tctacttgat gaatttacta cattaacaga agcaactcac tggtctggaa caacatatgg
     8521 tgaagaatct aaattaatta cagaatattc tgtacctatg ttggatattg agataggaag
     8581 tagtagtgat agttttaata atagtatagc aatacaggta ttggcaaaat cattaattag
     8641 agtatttgat tgtgatgaac ctttgaagac tttgttatgt gttggagggg tacattttga
     8701 gaaatctttc tcagatatta taaaaaataa agaatataac atatcaattg gacatgtatt
     8761 gccaaatcaa tggattgtaa gtggaatgta tgatgatgaa tctggatttg agaaactaga
     8821 aaagtgtata aatagcattg aaggaggaat tgattgtata gtttttcatg ataaattaaa
     8881 aggtacctat aaagagcaat gccgtaaact aggtgaaaaa cttaatgtac ctgtatttaa
     8941 acataaaata ttaaaaaatc caaaagactt acctatatgg tagtcttata cagttaatat
     9001 aacaataagt cggattaatt tttaagcgag gtcaaaaatg atattatctt attttgcatt
     9061 attaattaga gtagtgctat tagcttttga aagagtagtt gtaagattat taggtgatga
     9121 agaaggagat atttataaaa atatagcttc atcatttcta tttttcttta tagggggagt
     9181 atgtttactt ccatttagca taatggatag agttagtgat tggtcgtttt taataccatg
     9241 ttatataagt agtttagtct attcaattgg ttcagtagcg tatgtaacgt ctttagcaac
     9301 tggggaagtt tcacttgtta ctccaataaa tagtttaaat tcattgtttt tactgctttt
     9361 atcagttata tttttaggtg aaggtttaag tttagcaaaa gttgcaggaa tcataattat
     9421 gattggtgga gtgtttatat taaaaaaagt aagttctttt ttaaagaatg tctctgtaat
     9481 ttttaacaat ttaccatgta gattaatgtt tttatacata tttttgcagt cagtaggaag
     9541 agtattggat aaggcttttt ttgtacaagt atctcctata ttatattcaa ctatactgta
     9601 ttttttcgta ggtttaaatt tatttatctt tttagcattt aaaaagaaac aaaaaataat
     9661 ttgtgagatt tttactaata aaaaggggct ttcaatttta agtggtgcta ttaatggata
     9721 ttcttattta gcattattaa ttgctttaaa taatctagaa cttagtatag cagagccatt
     9781 ttctcaagta tctatgataa taactttaat acttgctcat tttatattca aagaaaatat
     9841 aaaagaaaaa ataccaggtt caattttgat actaattggt gggtggctac tgttacttta
     9901 attgacataa aaaatgaatt ataaaggtgc tattagttag tatcagaaaa tttaaaaaag
     9961 tattgacaat tcttttaggc aaatgttaat ataataatac aaaatagtat aaatagaaga
    10021 catgttactg gtaatgcagg caggatcgaa tatattgctt tgatttttca aggtgtttat
    10081 ataggttctg ccttttttgc atcttgaatg gccattcata aaaaattatg gagaaggaga
    10141 ttacaagatg aattataaaa aaactgccat tgacattatt gaacaagttg gtggttcaaa
    10201 aaatatttta gctttagaac actgttcaac tagactacgc tttaagttga ttgatagaag
    10261 taaagtcaat caagatgctt taaaaaaagt acaaggtgtt atgagtatag ttaatggggc
    10321 acaatttcaa gttgtcattg gaaatagtgt tgttgatgtg tatgatgaaa ttttaaaagt
    10381 ttgcccttta gatacaaatg atagtactgt aaaaactgaa gaaaaaataa acttaggaac
    10441 aagatttata gaatttatta ttaatatttt tcaaccactg atttttgcta ttgcaggagc
    10501 tggtatttta aaatcactgt tgatgttatt agcaatgttt aatattttaa aatcagattc
    10561 tactatatat acattattag ttgctatttc agatgcaaca ttctatttcc tacctcttat
    10621 ggtagctgta actactgcca atgtattgaa atgtaatcgt ttagtagcta ttgccgctgt
    10681 aggatactta ttgcttccag caacaacaac tgctttaagt gaaggtgttg aactatttgg
    10741 atttacaatt ccaaacattg cctataatgc acaggttttc ccggctatct tatgtgtgtc
    10801 tttcttggca acaatggaga aaatttttaa caagtattca ccaaaaccaa ttcgaacttt
    10861 ctttgttcct atgatgtctt tggcaattac tgttccaatt actctaacta ttttaggtcc
    10921 tttaggatat aacgttggta caatttttac aaccattatt ttattcttat ataacaaaat
    10981 gggattctta gttgtaggat tacttgctgc ttttcttcca tttatggttg caacaggaat
    11041 gcacaaagca ctaatacctt attcaattaa tacaattgga aagttgggtt atgaagcttt
    11101 atatatgaca tcttcattag cccacaatat ttctgaaagt ggtgcttgtt ttgcagtagc
    11161 cttgcgaaca aaagatgaaa cattaaaaca aacagcatta tctgcaggaa tttctgcctt
    11221 gatgggaatt acagagccag ctttatatgg tattacctta caacataaaa gagcaatgtt
    11281 aggtgttgta ttctcaagtg caatttctgg taccttttta ggattatttg cagtaaaagg
    11341 atttgtaatt gttggaccag ggcttgccag tatgagtatg tttattgacc caaacaatgg
    11401 aaacaatttg atttttgcga gtattggttt tgttattgca atattaggct cgtttattat
    11461 tacactgatt atttggaagg aagatacaaa tacagtagag tcatcaattg gagatgttgt
    11521 tgaagaagag ctagaagaag aaaaagaagt aaatatctta gtaactccag tagaaggaaa
    11581 agtaatagat ttaagtaagg ttaatgatga actatttgca agtaagactt tgggagatgg
    11641 agttgcaatt ataccaacca atggaaattt atacgcacct tgtgattcag aagttgtgat
    11701 gttatttgaa acaaaacatg caattggttt aagaacaaaa aatggggctg agattttgat
    11761 tcatattgga ataaatactg taagtatgaa tggagatgga tttaaaacat ttgttaagac
    11821 aggcgataat gtaaaagaag gagatttatt aattcaattt gatttagaca aaatctcaca
    11881 tgctaattta gattcaactg ttatgattgt aaacaataat ggagcagatt atgcttataa
    11941 agtgttaaac cagtcttatg gcaatgtaaa aaaaggaagt atcttgtttg atgtaaaggg
    12001 aggaatataa atatgagaaa atttccagaa ggatttcttt ggggaggagc tacagctgct
    12061 aatcaatttg aaggtggttg gaatttaggt gggaaagggt ggtctgtatc agatgtagca
    12121 aaggcacact ttgatgcaga tgtgaaagat tataaatcta ataatgaaat cacaacaaaa
    12181 gatatagaag aaggattagc acatcctgaa gatgaagtaa attatccaaa acgtcatggt
    12241 agtgattttt atcatcacta taaagaagat attgctctaa tggcagaaat gggatttaag
    12301 acatatcgaa tgagtattgc ttggtctcgt atttttccaa atggagatga taaagagcca
    12361 aatgaagaag gtttacagtt ttatgatgat gtatttgatg agttgataag ttatggaatt
    12421 gaaccacttg ttactatgtc acattatgaa ccaccattaa atatcgtttt aaactatgat
    12481 ggatggtatt cacgacaagt tatcaatatg tttgttaggt atgtagaaac tatctgtgaa
    12541 agatataaaa acaaagtgaa atattggctt acatttaatg aagttgattc tatgattcgt
    12601 catccgtaca ctactggtgg tttagttcga gaccgtttta aggataaaaa ctttgaggaa
    12661 gttatttttc aagctatgca tcatcaattt gtagctagtg ccattgcaac aaaaatttgc
    12721 catgagatta ttccaaattc aaaagtaggg tgtatgttaa caaaattaac gtattatcca
    12781 tatacatgta gaccagaaga tgttttggca actcaacaaa aaatgagaag tatttatgca
    12841 tatagtgata cgcaggtttt tggtgagtat ccagtatatt tactgtcgta ttttaaaaat
    12901 aataacattc aaatagtaaa agaagaacat gatgatgaaa ttatgaaaaa gtatccagta
    12961 gattttattt cattctctta ctatatgtct tcttgtgagg cagctgatac aactggatta
    13021 gatattacac ctgggaatac gttattagct gtaaaaaatc cttatttaga aatgagtgaa
    13081 tggggttggc aaattgattc aataggtcta cgtatatctt tgattgaatt atatgataga
    13141 tacagaaagc ctttatttat agttgaaaat ggattgggag ctaaagatat tttaactgaa
    13201 gataaaaaag ttcatgacca atatagaatt aattatttaa aagaacactt taaatgtatg
    13261 ttagatgcga ttatcgaaga tggcgttgaa ctttggggat atacttcatg gggttgtatt
    13321 gatttggtca gtgaatcaac gaaacaaatg tcaaaacgtt atggatatat ttatgtggat
    13381 gctgatgact atggaaaggg gacatataat cgatataaga aagatagttt ttattggtat
    13441 aaaaaggtaa ttgaaaataa tagtatagat tttaaatgac caatagaagg tgagcaaata
    13501 aaatgaacta cataataaag aaagttttga actcgagcgt tgttttagtt catgatgcaa
    13561 agtataatga atttattttg ctagggaaag gaattggata tggtaaaaaa acaggagaat
    13621 atatttgtgg ttccaatgat aatcaaatgt ttgtccctgt tgaaaataca aaatcaaaac
    13681 aatttttaga attaatggat gatattcctg tcgacatttt aaagataaca caggaaataa
    13741 tagtagaggc agaaaagtta ttgaactcat cttttaataa aaacttatac ttaatattag
    13801 cagaccattt taactttgca attgaaagga tgcgtaaagg aataaaaatt acaaatcgag
    13861 tattttggga aataaaaaat tattatccaa atgagtttaa ggttggaatg gttgcaattt
    13921 atatggtaga ggagagattg ggcattcaat taccagagga agaagcagct aacattgctt
    13981 tccattttgc aaatgctatg gcttcagatg gaaattcata tgatacgatt aagtatgcca
    14041 aagtaattgg agaaataatt aatatttttg ttttttcttt gaatagaacc ttagataaaa
    14101 agagtatgca ttatatgaga tttataacac atataaaatt ttttgtggaa agattttttt
    14161 cagatagtat gttaagcagt ggtgatgatt tattatttaa tcaaatgaaa aggtcaaatc
    14221 caaaagaaat gttaattgca ttaaaagtta gagatttttt agaaaagaaa tatggaaaaa
    14281 agttaacaaa tgaggaaata gctttcttgg ttgtacatat cgcacgagtt aagcaataga
    14341 gtgaattact aatattatct tatacgtcta catggtagaa aattgaaaga aggtaaagta
    14401 tataaagaaa ctgagacata ttattttgtt gttcattatg tttattttat gagtgtatcc
    14461 tgttaaaaat ttaagttaat atttataatt aaaatataca aa
//
LOCUS       DAMJYR010000092        14485 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.095, whole genome shotgun sequence.
ACCESSION   DAMJYR010000092 DAMJYR010000000
VERSION     DAMJYR010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14485
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.095"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            122..1750
                     /gene="aspD"
                     /locus_tag="PC471_002699"
     CDS             122..1750
                     /gene="aspD"
                     /locus_tag="PC471_002699"
                     /EC_number="4.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 4-decarboxylase"
                     /protein_id="HDF2789816.1"
                     /translation="MKNHTYKEIKNIYGKISPFEFKDKLIDIAKYTAKENNRELLDAG
                     RGNPNWTCSTAREAFFTFGHFAITETRSNWDLGHLAGMPQKKGIKERFFKFINENIDM
                     PGAYLARDIINFGINELGFDGDEFVHELADGIIGDNYPLPDRMLPHMEKIVHDYLVQE
                     MKYDISGKYGDVEIFAVEGATAAMCYIFDSLMANELLKKGDTIALMTPIFTPYLEIPN
                     LPRYDFKVVNINANEVDEKGAHTWQYTKKELEKLRDKSIKALFVVNPNNPASIAMDET
                     SCNNLIDVIENYNKDLMIISDDVYGTFVEDFSSLMSKLPYNTVGVYSYSKYFGVTGWR
                     LGTFALHKKNVFDKKINDLTGELKKSVDKRYSDMSLNPSSLSFMERVVADSRLVALNH
                     TAGLSTPQQVQMAFFSAFALIDKVDAYKKLNMSICHRRQKLLFEALELPINENKNNAA
                     YYTQFDIEEWAKLNYGEDLFKFISKTASPVDVLYKLANDYSVVLLSGNGFYGPEWSIR
                     ISLANLYDEAYTKIGKAIREILNGYIVEWQKIKK"
     gene            1871..2866
                     /locus_tag="PC471_002700"
     CDS             1871..2866
                     /locus_tag="PC471_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HDF2789817.1"
                     /translation="MSIKKKVAIVFTGGTISMTVDDKVGAAIPTLSGEQILSMVTNID
                     KVADVEVFNFDEIPGPHITPYRMMELKNYVNDLLARKDITGVVITHGTDSLEETAYFL
                     DLTIDNIKPVIVTGAMRSSSELGYDGSSNLSASVCTAVSKDAMGKGVLVVLNNEVLLA
                     SEATKTNTLSLNTFKSLTSGPLGIIDCNELVLTRDIVNRTIIDTDKVESKVALFKAYV
                     GENADFIRFAVDSGYKGIVIEAMGRGNIPPQMLSGVEYAREKGLPVVIVSRCHSGRVF
                     DSYGYFGSGRDLKNLGCIFGGDLPGQKARIKLILALGKTDNLDEIKDFFEKGIYC"
     gene            complement(3044..3427)
                     /locus_tag="PC471_002701"
                     /pseudo
     CDS             complement(3044..3427)
                     /locus_tag="PC471_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431032.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
     regulatory      3654..3751
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     repeat_region   3892..4450
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=3892..3921
                     /rpt_unit_seq="aatttacattccatatagttaatataaaac"
     gene            complement(4736..4894)
                     /locus_tag="PC471_002702"
     CDS             complement(4736..4894)
                     /locus_tag="PC471_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2789818.1"
                     /translation="MDNFLQGILASLSASLIVYIASKLFRKRKKPLKAATKSGWEFDL
                     KIRFHKTK"
     gene            complement(5188..6558)
                     /locus_tag="PC471_002703"
     CDS             complement(5188..6558)
                     /locus_tag="PC471_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp29"
                     /protein_id="HDF2789819.1"
                     /translation="MKLKKLLSLGLVSMFVAGFPISANALDKIDKIQGADKYETAGII
                     ADKQSYTTAILINADSTMADGLSASGLAGATNAPILLTKKNNIPNATLKRVEKAKKVY
                     IIGGESSIDKATETFLKDKGIETRRLQGNDRIKTSYNVAKEINSINKVNKVILTNAFK
                     GEPDAMSAAPVAVRDKAAIVLTDGKSVPFNTTGIESYAIGGTSSMSDSLVNDTNSTRL
                     GGVDRYDTNKKIVNKFYNGAKEFYIASGTDLVYALVASPMAKNNPIVLVDIGSNKDIL
                     KNAAKVTGIGNLSDKIMLECENTINNISLPSSNKNIICGLKIDFDSNHIIWQDKGSYL
                     ECYDDNLEYYTEKYKDPTVPINIENTSTQNLDVTKLKVYGEANSTLYMGQYAKIKMDF
                     NSFGVLKPGEKCTIYLDIISLDPSRSMTLTFSYVGDKENYKYEQGLASRILFYNPDGE
                     DFFEYK"
     gene            6774..7891
                     /locus_tag="PC471_002704"
                     /pseudo
     CDS             6774..7891
                     /locus_tag="PC471_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861579.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
     gene            complement(8254..8334)
                     /locus_tag="PC471_002705"
                     /pseudo
     CDS             complement(8254..8334)
                     /locus_tag="PC471_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005541856.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            complement(8374..9042)
                     /locus_tag="PC471_002706"
     CDS             complement(8374..9042)
                     /locus_tag="PC471_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HDF2789820.1"
                     /translation="METVTNNQSGVEIQLSLFEAIKDLLLINPIFLLGIILIGIFLVV
                     IFKKNSDIPKVKTSILSLAMYYYLCLTLSHIVGIPTFSEYIRLSQLGETFFNPNINLI
                     PFSDGFSLSFILNILLFVPLGFLCPLISKTFERLRNTFLIGFGLSLFIETIQLFTLHR
                     ATDIDDLLTNVIGTIIGYFCFKLIAKLRIVKLHSDDDSMEQDYTVCIPIAIIIVALIL
                     GFFS"
     gene            complement(9895..12315)
                     /locus_tag="PC471_002707"
     CDS             complement(9895..12315)
                     /locus_tag="PC471_002707"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HDF2789821.1"
                     /translation="MSVYNFKEVESKWQKIWKDNNQYKMDTAQTEKPNYYTLEMFPYP
                     SGKIHMGHVRNYSIGDVVARFKKMEGYNVLHPMGWDSFGLPAENAAIKHGIHPHKWTM
                     ENIEEMKEQLNLLGISYDWDKEVATSTPEYYRFTQEIFLKFLEHGLAYKKKSYVNWCP
                     SCETVLANEQVVQGACERCKATVLKKDLEQWYFKTTEFAEELLNDLDILDGWPEKVKT
                     MQKNWIGKSTGADLVFDIDGTDKSMTVFTTRPDTTYGVTYMVLAPEHELVKELVAGTE
                     YEADVEAFVQKMHTMTEIERTAADVEKEGMFIGRYVINPLNGKKVPLWIANYVLVEYG
                     TGAIMAVPAHDERDRDFAEKYNLDIIDVITEDNKMINSEEFNGLDASEGFEGIIDKLE
                     KEGRGKRTINYRLRDWLVSRQRYWGCPIPVVYCDECGIVPVKKEDLPVLLPTDVEFTG
                     KGESPLTTSKQFMSTTCPHCGKPARREVDTMDTFVDSSWYFLRYVDSNNENEPFSKEL
                     VNRWHPVDQYIGGVEHAIMHLLYARWFVKAFKSMGMVDFNEPFKNLLTQGMVLMDGSK
                     MSKSKGNTVSPMDIIDEYGADTARLFVLFAAPPERDLDWSEQGVDGCFRFLNRVYRLV
                     DELADVVKKDVEFGELNSQDKDMRYTIHSTLKKVTADLSEKFGFNTAISALMELINDM
                     YKYKELDNINEAVIKEGVQTIVTIIAPFAPHLGEELWTMIGKEGSVFDIDWPKYDEKA
                     LVKDEIEVVVQVNGKVRGKLTVNSNISKDEMEKVALEDEKIKGLVEGKTIVKVVAVPK
                     KLVNIVVK"
     gene            complement(12669..13019)
                     /gene="rsfS"
                     /locus_tag="PC471_002708"
     CDS             complement(12669..13019)
                     /gene="rsfS"
                     /locus_tag="PC471_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HDF2789822.1"
                     /translation="MTVEQMTKIAYDAIEDKLGQDTVIINIGKVSSLCDYFIITTASS
                     QRQVKAIADNVEDELAKLGLEPRGKEGQGTQTWVLLDYGDIMVHVFNEENRGFYNLEK
                     LWKDAPYIDIDTLA"
     gene            complement(13136..13708)
                     /gene="yqeK"
                     /locus_tag="PC471_002709"
     CDS             complement(13136..13708)
                     /gene="yqeK"
                     /locus_tag="PC471_002709"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HDF2789823.1"
                     /translation="MNLENINQRLNQMLPVGRLSHSKNVAKCAEKLCEIYGCDKEKAY
                     LAGMIHDCAKYLSDKEIEDYVNKYEIYLDPIEDGNRSLSHSVIGAYICEYEFEVEDED
                     IINAIKYHTTGREDMSLLEKIIYIADLIEEGRKFPVVDTLRELAYGGKLDEALLTSFN
                     NTLMFVINKKEEIHPRTVMARNYLIKEKLL"
     gene            complement(13709..14398)
                     /locus_tag="PC471_002710"
     CDS             complement(13709..14398)
                     /locus_tag="PC471_002710"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HDF2789824.1"
                     /translation="MRSENLVKMAELKNTEKLEKFNRHKGKIKIGILGGTFDPIHYAH
                     LATAEFIRDKYDIDKIIFIPSGNPPHKLCITTDKYDRYNMTLLATESNEDFLVSKVEI
                     ERKKRTYTIDTLKYLKKKYKNADIYFITGADAICSVEEWKDVKKNFELATFIAATRPG
                     ISLLRSQETIEKLTKKYNADIITVYVPSLDISSTYIREQLNEGKSIRYLVPENVENYL
                     YENKLYQYGDD"
BASE COUNT         4877 a         2372 c         1837 g         5399 t
ORIGIN      
        1 ttattttaat ttcatacttt actttttcta taaacctatt ataattaaag tataatcatt
       61 tttgcaactt gtttatttta ttataaaaat gtgaaactta ttacataagg aggaatttaa
      121 catgaaaaat catacttata aagaaatcaa aaacatatat ggaaaaataa gcccttttga
      181 atttaaggac aaattaatag atattgcaaa atacactgca aaagaaaata acagagaatt
      241 acttgatgca ggtcgtggaa atccaaactg gacttgttca actgctagag aagccttttt
      301 tacttttggt catttcgcta ttactgaaac tcgttctaat tgggatttag gtcatttggc
      361 tggaatgcct caaaaaaagg gtattaaaga aagatttttt aaatttataa atgaaaatat
      421 agatatgcct ggagcatatt tagctagaga tattataaac tttggtataa atgaacttgg
      481 atttgatgga gatgaatttg ttcatgaact agctgatgga attattggtg ataactatcc
      541 acttccagat agaatgttac ctcatatgga aaaaatagta cacgattacc tagttcaaga
      601 aatgaaatat gatatatcag gtaaatatgg tgatgtagaa atttttgctg ttgaaggtgc
      661 tacagctgct atgtgctata tatttgattc attaatggca aatgaacttc taaaaaaagg
      721 agataccata gccttaatga ctccaatatt tacaccttat cttgaaatac ctaaccttcc
      781 tcgttacgac tttaaagtgg taaatataaa tgctaatgaa gttgatgaaa aaggtgctca
      841 tacatggcaa tatacaaaaa aagaattaga gaaacttcgt gacaaatcta ttaaagcttt
      901 attcgttgtt aatcctaata atcctgcttc tatagctatg gatgaaacat cttgtaataa
      961 tctaatagat gtaattgaaa attataataa agatttaatg ataatatcag atgatgtata
     1021 tggaactttt gtagaagatt tttcatcctt aatgtcaaaa ctaccttaca acactgttgg
     1081 agtatattct tattcaaaat attttggagt tacaggatgg agacttggta catttgccct
     1141 tcataagaaa aatgtttttg ataagaaaat aaatgattta actggagaat taaaaaaatc
     1201 agtagataag cgttacagtg atatgagtct taacccttct agcctttcat ttatggaaag
     1261 agttgttgct gatagcagac ttgttgctct caaccatact gctggtcttt ctactccaca
     1321 gcaagtacaa atggcgtttt tctctgcttt tgcattgatt gacaaagtag atgcctacaa
     1381 aaaactcaat atgagtatct gccatagacg tcaaaaacta ttatttgaag ctttagagct
     1441 tccaattaat gaaaacaaaa acaatgctgc atattacact caatttgata ttgaagaatg
     1501 ggctaaatta aactacggag aagacttatt taaatttata agtaaaactg catctccagt
     1561 agatgttctt tataaattag ctaatgacta ttcagtagtt ttacttagtg gaaatggatt
     1621 ctatggtcct gaatggtcaa ttagaatatc tctagcaaat ctatatgatg aagcttatac
     1681 taagatagga aaagccataa gagagatatt aaatggttat atcgttgaat ggcaaaaaat
     1741 taagaaataa ttatattaca ctgaaatatc taaattatat gaataaaaga gtttatgtag
     1801 accttaacta ctttttttga tataatccta tcggaattat aaattttatt tatgaaaggc
     1861 agagaatagt atgagtatta agaaaaaagt tgccatagtt tttactggtg gaactatatc
     1921 tatgacagta gatgataaag tcggagcagc tataccaact ttatcaggtg aacaaatact
     1981 atcaatggtt accaacattg ataaagttgc agatgttgaa gtttttaatt ttgatgaaat
     2041 tccaggacct cacataacac cttatagaat gatggaattg aaaaattatg taaatgattt
     2101 attagcaaga aaagatatta caggtgttgt aataacacat ggtacagata gtttagagga
     2161 aacagcttat tttctagacc ttacaattga caacatcaaa cctgtaatag tcacaggtgc
     2221 tatgagaagt agttctgaac ttggatatga tggttcaagt aatttatctg catcagtttg
     2281 tactgcagta tccaaagatg ctatgggaaa aggagtcctt gtagttttaa acaatgaagt
     2341 attattagct tctgaagcta ctaaaacaaa tactctttct ctaaatacat ttaaatctct
     2401 aactagtggc ccattaggaa taattgactg caacgaactt gtccttacaa gagatattgt
     2461 aaatagaaca attatagata ctgataaggt agaatctaaa gttgccttat ttaaagcata
     2521 tgtaggagaa aatgctgact ttataagatt tgctgttgat agtggttata aaggtatagt
     2581 tattgaggca atgggtagag gtaatattcc tcctcaaatg ctttctggag ttgaatatgc
     2641 tagagaaaaa ggacttcctg tagttatagt ttctagatgt cattcaggta gagtattcga
     2701 tagttatggt tactttggtt ctggtagaga tttaaaaaat cttggttgta tatttggtgg
     2761 ggacttacca ggtcaaaaag ctagaataaa acttatctta gcacttggaa aaactgataa
     2821 ccttgatgaa atcaaagatt tctttgaaaa aggaatctat tgttaattct aataacttct
     2881 atttaattat ttattgatat ttcaacattt ttaaatcaaa atctaatact tatgtcagtt
     2941 aaatgtcagc aaaatatttt atccacaagt tttccacaac ttatccccaa tattatccac
     3001 aagaaaaaag gtagcactta tattagtagc taccttttat ttattaatct tcatctttcc
     3061 agctttttat ccaatttcct atttctatta ttttttcttc agttatttct tctccatcat
     3121 gcaattttga aattaattct gataatggat tgttatgtag acctccaaaa attctttttc
     3181 cctctatttt gagatattct ttctctttta ttaatactct atagtgtaca tgtttcccaa
     3241 ttctctgcga tatgaaaaaa ccttttttag ttaatctagc taaaagtgta attgtagttg
     3301 tatgcttcca agcatatgtt ttttctattt catctgcaac atcttttgat attacagttt
     3361 tataaccagt attctatatg tatttcataa tttttaattc tgcatctggt aattttttat
     3421 tcaacataat atattccttc tttcaattta tgtcacttcc tcaaacttat ctagcttaaa
     3481 ttaataatag gagcttttta tcacaaaaac tacaggtaaa tttttctata tattctacgt
     3541 atttagtctg ttttttaagt gtgaaattac gttcatacct ataaatttat tcaattttca
     3601 aacaatctta ttgaataata actaattcta ttgtattatt tagttataga ataaaactaa
     3661 atcggcaaaa ctagagaaat ctagtgacgc aaagctatag agactaagac ttatataaat
     3721 atcttatgag ttatgtcagc cagttgccaa agagatattg ttctttttgt ttttatgaaa
     3781 gttttttagg gggataataa tttatatatt tagagataaa atagataaat gtacacatat
     3841 attgactgct tatattagca gttcgtatga ttattgtaat tttttggata caatttacat
     3901 tccatatagt taatataaaa caagtgtttc gaatttcttt tctgaaacta taccaacaat
     3961 ttacattcca tatagttaat ataaaacgtt atatactgaa agcggtgaaa ctggttacgt
     4021 agcaatttac attccatata gttaatataa aacctgtgcg tgtaggagat ggtaacacaa
     4081 ctgttgaaga atttacattc catatagtta atataaaaca cacatctaca tgtttttcta
     4141 tctgagttca tctctaattt acattccata tagttaatat aaaacttttt aatccttctt
     4201 ctgaactccc aaagtttata tcatttacat tccatatagt taatataaaa cccacgaaat
     4261 ttaaggtcga tatatcagat ttgaaaaaat ttacattcca tatagttaat ataaaacaag
     4321 ctaaatggag caaccgacca agtcgctaga aataatttac attccatata gttaatataa
     4381 aacttttcta ttaagtgtaa gcagaatgca tggaatatga aatttacatt ccatatagtt
     4441 aatataaaac cccaaaataa attgagtatt tccaatgcct acacatatac aaatctctca
     4501 aatttgcagt gaaccatgag tagtgcaatt gataacactt atcacaagtc ctcaatgcct
     4561 tatatttcaa ctgttaagcc ttaatttctt caaaaaatcg aacactgcaa aatttctatg
     4621 actttattat atcataaaaa tattattttt gaatatctgt accaatttgt gatataataa
     4681 aagcaagaag aactacaatc tattttgctg tagagtggag ttcataattt agaaattact
     4741 tagttttatg gaatctgatt tttaaatcaa attcccaacc actcttagtt gccgctttga
     4801 gtggtttttt acgctttcta aatagtttgc tagctatata aactattaag ctagcagata
     4861 gacttgctag tatgccttgt aaaaaattat ccatgtatat tcacctccct tcttaacgtc
     4921 gggaggataa ttttttgata catgaactcc actctataaa ttgtagatta catcttcttg
     4981 ctaaaaatat tataacatat aattcttaca tattttacct atttattact ttttacatta
     5041 taataatatt cttttaatca aaaaaacacc tactattaaa gtaaatgttt ttaagtattt
     5101 ttaattttaa aatccatata gttcatatga aactaaatat aataactcaa catatgagca
     5161 atatcgagac atttacatac ccactagtta tttatattca aaaaaatctt ctccatccgg
     5221 gttataaaat aatattctac ttgccaatcc ttgttcatat ttataatttt ctttatcacc
     5281 tacataagaa aatgttaatg tcatgcttct tgatggatct aatgaaatta tatctaaata
     5341 aatagtacat ttttctcctg gctttaatac accgaaacta ttaaaatcca tttttatttt
     5401 agcgtattgc cccatatata atgttgaatt agcttctcca taaactttta attttgttac
     5461 atctaaattt tgtgtacttg tattttctat atttatagga actgttgggt ctttatattt
     5521 ttcagtataa tattcaaggt tatcatcata acattcaaga taacttcctt tatcttgcca
     5581 aattatgtga tttgaatcaa aatcaatctt taatccacat attatatttt tatttgaaga
     5641 tggaagacta atattattga tggtattttc acactctagc ataattttat cacttagatt
     5701 tcctatcccg gttacttttg cagcattttt aagtatatct ttattacttc caatatcaac
     5761 tagtacaata ggattatttt tagccattgg tgaagcaact aaagcataaa ctaaatcagt
     5821 accactagct atataaaatt cttttgcgcc attatagaac ttatttacta tttttttatt
     5881 agtatcatat ctatctactc cacccaatcg agttgaatta gtatcattta ctaaactatc
     5941 actcattgat gaagtgccac ctattgcata actttctata ccagttgtat taaatggaac
     6001 actttttcca tcagttaata ctatagctgc tttatctcta actgctacag gtgcagcact
     6061 catagcatct ggctcacctt tgaaagcgtt agttaatatt actttattta ctttattaat
     6121 ggaatttatt tcttttgcta cattataact tgtcttaatc ctatcattac cttgaagcct
     6181 tctagtctct attcctttat cttttagaaa agtttctgtt gctttatcaa tggaactttc
     6241 tcctcctatt atgtatacct ttttagcttt ttctactctt ttaagagttg cattaggtat
     6301 attattcttt ttagtcaaaa ggattggtgc atttgttgct cctgcaagtc cactcgcact
     6361 tagcccatca gccattgtac tatctgcatt tataagtatt gcagtagtat aactttgttt
     6421 gtcagctatg ataccagcag tttcgtattt atctgctcct tgaattttat caattttatc
     6481 aagtgcatta gctgatatag gaaaaccagc aacaaacatt gacactaacc ctagtgataa
     6541 taatttcttt aatttcacaa tatctccccc ttttatatat gaaatactat atatcttaat
     6601 aattctacat taaattttct tttcctcctt taacgatatt tatcttcctg ttaatttaag
     6661 tgtatacaaa tacgtgttat aatgtaactg taaagaatta aaaagataat gacataatta
     6721 gagtagtaca actgttcttt tataaaacta acttataaag ggagcgtaca tatatgaata
     6781 tcaaatcagc ttttataaga aaaagagggg aaaaattcta tgtatatgtg gagtacatag
     6841 aggaaacaac tggcaagata aaacagaaaa gttatggaag ttatgaaaag aaaaaggatg
     6901 ctgaaaaaca tttaattgaa ataaaatcta ctataaacaa taataagttt gttgctccaa
     6961 aagatatgac atttgtagaa agatgttata aatacatgga catgaataaa aataattggt
     7021 ctccatatac tgttacaaat acaaaatctt gggttaaaaa ttacatagaa cctcttttta
     7081 agaatctaaa actcacagaa gtaaatccta gttcagtaca agcttttgta aattataact
     7141 ttaataattc tacctttact agtgctaaag taaggtatag ctttctaagt tctgtaataa
     7201 gggaagcata tagattaaga gaaatacaag aaaattcatg tgattttata aaacttcctt
     7261 ctaaagaaaa atattctaca aatgaaatat acaatagaaa agaagtatta ttgctaatag
     7321 aaaagttact tagtaataat atagaaatac ctattctttt aatgttactt ctaggtttaa
     7381 gaatgggaga agctactggg cttaggtggt cagatgttga cttagataac aatctgataa
     7441 atgttaatca aattcttgtc tattctaatg gcaaaatatc ttttaaagag ccaaaaacac
     7501 caaaatcaaa aagagcttta tcagctccaa aagaattaat tgaaaaattg aaaattaaaa
     7561 aactaagaca aagcaaatta aaattgtaag gtatcctgga aaacagaaat aatttagttt
     7621 gtctaaacac caatttaaat ccgtttctac cttcttcact aagaaaaacg tttcacaatt
     7681 ttacaaaaaa aataatttaa aaaagattag aatacatgat ttaagatata caagtgccac
     7741 tctgctgctt ttagggggaa ctaatatgaa agttgtttct gaaagattgg gtcatacaga
     7801 tataaaaata actatgaata gatattctca tgtacttaaa gaaatggaca aagaagcttc
     7861 cgacaatcta agcgaacttc tatttaaata gtaaaaactg actgtcagtt aaatgtcagt
     7921 taaacctaaa aaaataggtt ttgtcagttg attatcagtc aattatttta taatagtttc
     7981 acttaaaaac gtctatatct acacatttta ctatttgttt atgcttatta atatctatct
     8041 gttattatta attctaatag cttctattta attatttagt tcagtttaca ggaaactaca
     8101 cacaaaccat aaaataggat gttgacaata taacagatag tatgctcctg taaaaggtaa
     8161 cgtactatct gttatgttgt ctggatttcc aaatggttac cctttggcac acaactttgc
     8221 tatctccttg gtaaactaaa agtttgtaat ctaaaatcct aatccatctc tataaaattt
     8281 aattgacttc tccatacttt ttacacctaa acatataaaa gtaattttat tcacttaatg
     8341 acctccataa ttcccaaatt agctatttgg aacttaacta aaaaatccta atataagggc
     8401 aactattata atggcaatcg gaatacatac tgtgtaatcc tgttccatag agtcatcatc
     8461 tgaatgtagt tttacaattc gcaatttagc aatcagtttg aagcagaaat agcctattat
     8521 tgttccaatt acattcgtga gtaagtcgtc tatatcggtt gctctgtgta acgtaaaaag
     8581 ctgaatagtt tcgataaaaa gagataatcc aaaaccaatc aagaatgtat ttctcaaacg
     8641 ttcaaaggtt ttgctgataa gcggacataa aaaacctaat gggacaaaaa gtaaaatatt
     8701 caaaataaaa ctcaaactaa atccatcact aaaaggaata agatttatgt ttggattaaa
     8761 gaacgtttcc cctaactgtg acagtcttat atattcactg aatgtaggga taccaacaat
     8821 atgcgatagt gttaaacata agtagtaata cattgccagt gataaaatag atgtttttac
     8881 ctttggaata tcactatttt tcttgaatat tacaactaag aaaataccaa tcaagataat
     8941 tcccaacaag aaaataggat taattaacaa taaatctttg atagcttcaa acagactgag
     9001 ttgaatttct acacctgatt gattatttgt tacagtctcc atttgtacat acctcctcat
     9061 aacatttcaa ttatttctaa aacacaaaat agaagataaa tcttaaaatc atcctatcaa
     9121 aatttttaaa tttgccttta tcagtttata ccaccagtta attcctgtct tacttctgta
     9181 tggtattctt gggattcttt catcaaatta gacaaaaaat ggttaccata tcaataaact
     9241 ggttacgctc catatctccc gaagcaacca tcccctgaga ataaatccgt atcaggaatc
     9301 tccacatcta ctgcacctat cacaagttat ttgataacat cctcagacaa tcccaacttg
     9361 gatatatcga caacacccat tagaatacca ctgctgacca tacttgtttt cctgctgata
     9421 attttaatca actgggaagt tgaaacgcta tcttttttac aagttccttt ttagaaacat
     9481 ggtagctatt atatgaatct gaatttcgtt gtctgggtgt ttcatataaa gcatagatac
     9541 gcaccttttt tgcctgtttt cattatatag aaaaaaattt caaaatacaa taattatatt
     9601 tttttcgttt aagcttagag cagttccttg atgaacaaca ctttggaaac tgaatgattt
     9661 acataccact taatacatta actcgattta acattaatta taataatatt taaaacaaca
     9721 actagggtat ataagaatga actttttagt tctttaatca tttttttgta taaaatcaaa
     9781 tctattttta taatttcaac aattttataa tttcaacaat gaaaaaaggc ttatctctat
     9841 tttgagacag cctcgtttat tttataaatt tatatttttg ttcatatttt tgagttattt
     9901 aacaactata tttactagtt ttttaggtac agctacaact ttaactatag ttttaccttc
     9961 aactaatcct tttatttttt catcttctaa agctaccttt tccatctcat ctttagatat
    10021 atttgagtta acagttaatt ttcctctaac ttttccatta acttgtacaa caacttctat
    10081 ttcatctttt actaaagctt tttcgtcata tttaggccag tctatgtcaa atacacttcc
    10141 ttctttacct atcatagtcc atagttcttc acctaaatga ggggcaaatg gtgcaattat
    10201 agttactata gtttgtacac cttcttttat aactgcttcg tttatattat ctaactcttt
    10261 atatttatac atatcattta ttaattccat aagagctgat atagcagtat taaatccgaa
    10321 tttttcactt aaatcagcag taactttttt aagtgtagag tgtatagtat atctcatatc
    10381 cttatcttgg gaatttaatt ctccaaactc tacatccttt ttaaccacat ctgctaactc
    10441 gtcaaccaat ctataaaccc tatttaagaa tctaaagcat ccatcaacac cttgctctga
    10501 ccagtccaaa tctctttctg gtggtgctgc aaataatacg aaaagtcttg cagtatctgc
    10561 tccatactca tctattatat ccataggaga tactgtattc cctttagact tactcatttt
    10621 agaaccatcc ataagtacca ttccttgagt aagtaagttt ttaaatggct cattgaagtc
    10681 taccattccc atactcttaa atgcctttac aaaccatctt gcataaagta aatgcattat
    10741 agcatgttct actccaccta tatattggtc tactggatgc catctattaa ctaattcctt
    10801 actaaatggt tcattttcat tattgctatc cacatatctt aagaaatacc aagaagaatc
    10861 aacgaatgta tccatagtat caacttccct tctagctggt tttccacagt gaggacaagt
    10921 tgttgacata aattgctttg aagtcgtaag tggagattcc ccttttccag taaattcaac
    10981 atctgttggt aaaagaactg gtaaatcttc ctttttaact ggaactattc cacattcatc
    11041 acaataaacc acaggtatag gacaacccca gtatctttgt cttgaaacta accagtctct
    11101 aagtctataa ttaattgttc tctttcctct accttctttt tctaacttat cgattattcc
    11161 ttcaaatcct tctgaagcat ctaatccatt aaattcttct gaatttatca ttttgttatc
    11221 ttctgttatt acatcaatta tgtctaaatt atatttttca gcaaaatctc tatctctttc
    11281 atcatgtgct ggaactgcca ttatagcacc tgttccatac tcaactaata catagttagc
    11341 tatccataga ggaactttct tgccatttaa aggatttata acatatctac ctataaacat
    11401 tccttctttt tctacatcag ctgctgttct ttctatttct gtcatagtat gcattttttg
    11461 aacaaaagct tcaacatctg cttcatattc agtccctgca accaattctt taactaattc
    11521 atgctctgga gcaagtacca tataagtaac tccataagtt gtgtctggtc tagttgtaaa
    11581 tactgtcata gatttatcag ttccatctat atcaaaaact aaatctgctc ctgtactttt
    11641 acctatccaa tttttttgca ttgttttaac tttttctggc catccatcta atatatctaa
    11701 gtcattaagt aattcttctg caaattcagt agtcttaaaa taccattgtt ctaaatcttt
    11761 ctttaatact gttgctttac atctctcaca tgctccttga acaacttgct catttgcaag
    11821 aacagtctca caagaaggac accagttaac atatgatttt ttcttatatg caagcccatg
    11881 ctctaaaaac ttcaaaaata tttcttgtgt aaatctatag tattctggcg ttgaagttgc
    11941 aacttcttta tcccaatcat aactaatacc taataaattt aattgctctt tcatttcttc
    12001 tatattttcc attgtccatt tatgtggatg tattccatgt tttatagctg cattttcagc
    12061 tggtaatcca aaagaatccc atcccattgg atgaagtaca ttatatcctt ccattttttt
    12121 gaatcttgcg acaacatcac ctatagaata atttctaaca tgtcccatgt gtatttttcc
    12181 agatggataa gggaacattt ctaatgtgta ataattaggt ttttcagttt gtgcagtatc
    12241 cattttatac tgattattat ctttccagat tttttgccat ttcgattcta cttctttaaa
    12301 attatatacg ctcatatttt gcctcctatt tttaattgtg tataactacc ttatttagta
    12361 ttatttactt ataatttcca aataattctt tgaaaataaa aaatcctcgt cactaaactt
    12421 acgtttaggg acgaggataa atcttcgcgg taccacccta attagcctac tacatatata
    12481 gactctctta gatagctttt aatccagcca tggagaatag attttaatct agtaagttca
    12541 aagttatata atattagttc tcaccttcca ctaactctct gaaaaaatat aatcttttac
    12601 tgctctagtc tattcaattt aatataattt ttgaaatttt ctaattataa taacatatat
    12661 tatcatgttt atgctaatgt gtcaatatct atataaggtg catctttcca taatttttct
    12721 aaattataaa accctctatt ttcctcatta aatacatgaa ccattatgtc tccataatca
    12781 aggagtaccc aagtttgagt tccttgtcct tctttacctc ttggctctaa tcctagtttt
    12841 gctaattcat cttccacatt gtcagcaata gcttttacct gtctctgaga agatgctgta
    12901 gtaataataa aataatcaca caaactagaa acttttccta tatttattat aactgtatct
    12961 tgacccagtt tatcttctat ggcatcatat gctatttttg tcatttgttc cactgtcata
    13021 taaaaattcc tcactttcct aattaaaata cacttagtta ttgtaacata ttaatacgat
    13081 tttgtaacat agtcaaattt aaatttatta atatgtttgc ctttatatag cttttttata
    13141 ataacttttc tttaattaga tagtttcttg ccataactgt tcttggatgt atctcttctt
    13201 ttttattgat gacaaacatt aaagtattat taaaagaagt taaaagtgct tcatcaagct
    13261 tccctccata tgctaattct cttaatgtat ctacaactgg gaattttctt ccttcttcaa
    13321 ttaaatcagc tatgtaaatt attttttcta aaagagacat gtcctctctc cctgttgtat
    13381 gatatttgat tgcatttata atatcttcat cttccacttc aaattcatac tcgcatatgt
    13441 acgccccaat tacactatga gacaaggaac gatttccatc ctctattggg tctaaatata
    13501 tttcatactt atttacataa tcctctattt ccttgtcact taaatacttt gcacaatcat
    13561 gaatcattcc tgctaagtaa gctttttctt tgtcacatcc ataaatctca cataattttt
    13621 cagcacattt tgcaacattt tttgaatggc taagcctccc cactggtagc atttgattta
    13681 gtctttggtt aatattttct aagttcattt aatcatctcc atattgatat agtttatttt
    13741 catataaata attttctaca ttttcgggta ctaagtaacg tattgacttg ccttcattta
    13801 gctgttccct tatatatgtg gacgatatat ccagtgatgg aacataaact gttataatgt
    13861 cagcattata cttcttagtt agtttttcta tagtctcttg tgaccttaac aaactaattc
    13921 ctggtctagt tgcggctata aaagtagcta actcaaagtt tttctttaca tctttccact
    13981 cttcaacact acaaattgca tctgcacctg ttataaaata aatgtctgca tttttgtatt
    14041 ttttctttaa gtattttaat gtatctattg tataagtcct tttctttctt tcgatttcta
    14101 cttttgaaac taaaaaatct tcgttgcttt cagttgctag aagtgtcata ttatatctat
    14161 catacttatc tgtagtgata cataatttat gtggtggatt tcctgatggt ataaagatta
    14221 ttttgtcaat gtcatacttg tctctaataa attctgctgt tgctaaatga gcatagtgta
    14281 tagggtcaaa agtacctccc aatataccta ttttgatttt gcctttatgg cgattaaatt
    14341 tctctagttt ttcagtattt ttcaactctg ccatcttaac tagattttca cttctcatat
    14401 tttgtctcct tttaatgtta taatactatt gtaccattat tttttatttc ttaaagctta
    14461 taatttgttt ttttgggtat ataat
//
LOCUS       DAMJYR010000093        14477 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.096, whole genome shotgun sequence.
ACCESSION   DAMJYR010000093 DAMJYR010000000
VERSION     DAMJYR010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14477)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14477)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14477
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.096"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(139..1284)
                     /locus_tag="PC471_002711"
     CDS             complement(139..1284)
                     /locus_tag="PC471_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016034203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kelch repeat-containing protein"
                     /protein_id="HDF2789825.1"
                     /translation="MATKLEENAKLREVIDTLETTKDDLQTVKNNIADVLGLPFTGND
                     KLETTKNTLSSIRSTFVSNLSKKGVSTVSNAPFKTLAENVGKIEQGNMIVPPWYVPKN
                     IFIKALSTAVKDKTPCVSAEKKIYFINGYTGTSGLNNLCYDTVLNTTTTKNIPSSFVE
                     SALFFYDGYIYKIGGFSGYSAPTAICQKYNILTSSWTEIANMPTARGSACCEMYSNQI
                     HVLYGGEDSSSSQAVRVVEYYNTTLNTWSTKSTIIDSKQSHYFVCAKVSTYKIVVFGL
                     KSSISSHYFSTINYDPVSGTYSYAGEGFDSNTFAVQLGSTLYVSDYGYKAGSGKCGVV
                     IFSSSGIKKLTFDKGAVPNYSYFGASVDGRQIYFILNSEITCFIPEL"
     gene            complement(1301..2377)
                     /locus_tag="PC471_002712"
     CDS             complement(1301..2377)
                     /locus_tag="PC471_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDF2789826.1"
                     /translation="MAIDKSYYTIITDVGKAKIANASVTGNKVGFVKIQLGDGGGSEY
                     TPTESQTALKNVVWEGNIGNTTTDETAPNCIILESLIPSSVGGFMIREIGYLDDENNL
                     IAISKYKECYKPSIEQGAVVDMKVKTVLIVSNVNNIELKIDPTIIFATLKDIQDLETK
                     IGTVNTKIDTTKTELTSNIETAKTELNTKIDQLIAGGSNVAYTQRVAIDDWVEDAESG
                     FKATVTHSLLTQRIVVNIIDATTKENVVTNFKIIDDNSIEIRSEARSELNVYVINGNA
                     ETHFINATVDDNRVSEMTTYSSKKIEDSISSIQLIDTSISITDANDRFTSDKLDGVLE
                     EIMVEISGQRTKGITIVNNLIDMI"
     gene            complement(2389..3006)
                     /locus_tag="PC471_002713"
     CDS             complement(2389..3006)
                     /locus_tag="PC471_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmfQ family protein"
                     /protein_id="HDF2789827.1"
                     /translation="MDKEINLINYLPQILQDKEEYIKVFNVENKEIKTLHEKLNDLSS
                     DQFLEDLTPSGIKRWEKIMSITPKSNESLEDRRFRIFSKYISKLPYSERFLRNWLDSI
                     VGEGNYELTINNATYNIHLESDARNQDWFEEVHSFVSSIKPCNMTLDYTRVLVSKDNY
                     MNFGITTLIGQEITIYPWSPPDIETYGEIDVLTGNGVGYQEITIF"
     gene            complement(2999..4048)
                     /locus_tag="PC471_002714"
     CDS             complement(2999..4048)
                     /locus_tag="PC471_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HDF2789828.1"
                     /translation="MFELMTFENIIKRMLDSVPDTFDKREGSIIYNALAPVAIELTET
                     YIAMDELLDQTFVDTASYYYLEKRCKERGITPLPATNTIAKGVFNIDIPIDSRFNLGE
                     YNYVAIERISEKTYKMKCETTGPVFELGQLIPIEYVDKLETAELTEILINGEDEESED
                     SLRQRYYDSLNSQSFGGNMQNYKDEVNKIQDVGGVKVYPVWDGGGTVKLVIINSNFKV
                     PSSDLVNLVQEEIDPLQNQGEGLGLAPIGHRVTVEGVTSTTINISAEITYKNGYTWEN
                     IKSIAEEAIDDYLNELNMSWEDEENLIVRISQIETRLLSIDGVLDIANTMINEVKSNL
                     TIDSNSIVVRGEVVG"
     gene            complement(4049..4468)
                     /locus_tag="PC471_002715"
     CDS             complement(4049..4468)
                     /locus_tag="PC471_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2634 domain-containing protein"
                     /protein_id="HDF2789829.1"
                     /translation="MLPSDNLDYDIEDVSIINFDVRQEPSKTFKLNIEKSKIDGICDD
                     VEALKQTIFLILNTERYQHLIYSWNYGVELNDLIGEPISFVIPELERRIKEALIQDDR
                     VENVDNFEFQNVKGKVHCKFTVYSKYGNIKAEKVVSV"
     gene            complement(4473..4733)
                     /locus_tag="PC471_002716"
     CDS             complement(4473..4733)
                     /locus_tag="PC471_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2577 domain-containing protein"
                     /protein_id="HDF2789830.1"
                     /translation="MSQDLLQIIKKAAMDAVETSNPMRVVFGTIESINPLRVKIEQKL
                     SIGEIFLIQTDTFKRYTDKKIGDKVVLIRMQGGQQYLVLDRM"
     gene            complement(4747..6639)
                     /locus_tag="PC471_002717"
     CDS             complement(4747..6639)
                     /locus_tag="PC471_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HDF2789831.1"
                     /translation="MADDLVLANDRDVRLIIAHWEDFYEPAVIDGITWEIERRGTPSK
                     LEFTIVMDDILEFCEGNSVRLYYKGVGIFYGYIFQKKRDKENHIKIVAYDQLRYFKNK
                     DTYVYSNKTASELVKMLAKDFNLKYNVIEDTKYKLSRVEENKTLFDMILTALDDTLRE
                     KKEMYVLYDDFGRITLKNVASMKLDTVMNNDVIEDFDYNSSIDSDTYTKIKLVRDNEE
                     TGKRDVYIAQDSTHMRSWGILQMFDTVDKNMSEAEIKQKCDILLKLYNKKTKSLSLKN
                     VLGDIRVRAGCLVPVFLDLGDIELQNYMLVEKVKHTFENNSHFMDLTLVDGDEFASYS
                     SSSYSSGNTNNKDEKKNGPAQSITKKNTGKKVPAIFTAYYPGNNAMEGGKTDCNGKPL
                     DVKSRTVAGPMNREGVKKTWYTDDFLKKHPVFEYGDKVKIILPGTAYDNKVYTVKDNG
                     GRIYVETNGTYHIDILLANASECKKFGRKNGYIIIGGDEEQTYQVEGNNQSSTNNNSK
                     EDKLISIAKSKLGCNYVYGAEGPNNFDCSGFTQWCYKQIGIKIPRTASAQSKAGKAVD
                     LKDRSKWKAGDLLCRIGGGSSNHVVMYIGNNQIIHSPQTGDVVKIESVNSYRKGKAYT
                     HVRRFI"
     gene            complement(6632..7333)
                     /locus_tag="PC471_002718"
     CDS             complement(6632..7333)
                     /locus_tag="PC471_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HDF2789832.1"
                     /translation="MAYDFYLDGVQLPIPPPKLEIKVTNKNKTVDLINVGEVNILKKE
                     GLSEISFEAEFTHNKLPFCRGQFRDVQFFLSKLELLKTDCKPFQFIVSRELGNKVLFN
                     TNMKVSLEEYNIVEDAENGSDTKVAIKLKQYRDYSTKKLVPATPEKTNYGRTPPPVMK
                     PKEFRPDSSNKPNGKTYTVKAGDSLWLICKKQLGNGSLYKKVYELNKSMMDKANKGKN
                     LSKYTIYKGQVLKLG"
     gene            complement(7349..9742)
                     /locus_tag="PC471_002719"
     CDS             complement(7349..9742)
                     /locus_tag="PC471_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HDF2789833.1"
                     /translation="MATIQTSIKIFDGMTPAFRNMTNSINTTINSLERLQGRLNNPLN
                     AGNIQASQQSLNNIESILTRIEQKIGRNTNEQENFNNKIRQGSEAGSLLVSKLKSIAG
                     IYIGIKGIESITKAADTIASTKARLNLMNDGLQTTDQLNKMIYLSAQSARASYADTAA
                     QIAKLGILAGDAFGSSAEVIKFTELMNKAFVIGGTSANEASAAMYQLTQAMGAGKLQG
                     DEFRSIMENAPLLATKIADAMGKTKDQLKELSSSGAITADVIRNALFKASDEIEKKFA
                     SMPITFSQALTMMKNDAYMIFSETLSKISGALQSVRFSEIVVSMRNVMIAISSNIYDT
                     LNIIKNILNSDFFSDFVQGITTGTVLIIQGLGWITNAALNVANVFAQNWSIIAPAVYG
                     VVAAIAIYKGILLASAIVTGVSSFVNALYALGAYKACAALAAQELALFGKISAQTIEA
                     MVTAQATAAQYGLNAALYACPLVWIVLGFIAVIAVIFMVVAAINKFAGTSLTVLGVIV
                     GAVFAAVATIQNIMIWLFNRCVDVNEGIANGWNQCVYLMKQAIAKGVIFIIEKMASLN
                     DSVNNAGNALGKAFVDGANIAIRGVNKLIDLINKIPGINIGKVGEATFTPVKADNSYI
                     KQQIDSLNRWVGDAPEKVKLERMGYKDIGAEFQKGNALGTKWQNAISNKLKDTFDINK
                     MLEDAKDKLGLKDLWDKDNPLNNLGGFGGDLGKNVKDTAGNTAKMAKTMDKSQEDLKY
                     LRDIAEQETINRFTGVNIKIDMNNTNNINSEADVDGIVNVLTEKLNDAMVVSAEGIV"
     gene            complement(9807..10322)
                     /locus_tag="PC471_002720"
     CDS             complement(9807..10322)
                     /locus_tag="PC471_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HDF2789834.1"
                     /translation="MFCSNCGYEITGAGKFCSNCGTATLADKVNNDDLFINVHGKELN
                     LTNIYKETKGDKILAIDIAMKLLGLDIKECKNIIYPAFKELSEKINIEEEKEILREEE
                     YKQTNVLEDDVARCPRCGSVSLSAHKKGFGIGKAVAGATIAGGIGLVAGNLGAKKVRV
                     TCLSCGKQFWA"
     repeat_region   10453..11340
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=10518..10546
                     /rpt_unit_seq="atttacataccacatagttaatataaaac"
     gene            complement(11416..11565)
                     /locus_tag="PC471_002721"
     CDS             complement(11416..11565)
                     /locus_tag="PC471_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2789835.1"
                     /translation="MHENLLDIDRIELIKELGNIFEKMKNENPDEFYRFVSLVKEECR
                     KKREK"
     gene            complement(11617..12432)
                     /locus_tag="PC471_002722"
     CDS             complement(11617..12432)
                     /locus_tag="PC471_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin Bro"
                     /protein_id="HDF2789836.1"
                     /translation="MMNELMNFEGKDIEVFEFEGQILFNPKHVAECLDISDVNSSIRK
                     FNDNQVVKLTNSKMHNMQFRKLHNTGENFLTESGVYKLIFKSKKEEAERFQDWISDEV
                     LPAIRQTGAYITNNADPEKLREKASEIEKLQLAYNSTSMLKELLDGAGFDNKSKLLTA
                     KTLYKKAGIDLPIEINEEEHYFDTKQIASKLKLYSKSNKPAQMAVCEIIKKIDLEDSE
                     VKGVWETNGSWTGTVNKYTESVIDKIRNWIEENNRPTKIAGEKKNYHVVYKIE"
     gene            complement(12550..12732)
                     /locus_tag="PC471_002723"
     CDS             complement(12550..12732)
                     /locus_tag="PC471_002723"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HDF2789837.1"
                     /translation="MSNKLGRPPKENSKKLRFEVRLNQEQADILNECAENLKISKTDV
                     VIRGIELVKEKIDKNK"
     gene            complement(12919..13443)
                     /locus_tag="PC471_002724"
     CDS             complement(12919..13443)
                     /locus_tag="PC471_002724"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2789838.1"
                     /translation="MWGKFKKLSLLKKILVIFLIYFVVFTVSMMIHQAIRDSKNRDEV
                     NEGNITKENIISEREKEDIYKQEMQAKVDSMIPEDLKDKTTYYVNILNPTKGEGYIVS
                     IQVENSRFNDENECRNFTKEFVNNIKDMNDIHSVRISFIVDVTLTYNVFLDDWNNIKN
                     NVNLIDDLDFSSGN"
BASE COUNT         4422 a         2743 c         1620 g         5692 t
ORIGIN      
        1 acttttgtaa tctttgaatt tactacattg tatattaaag taaattaatt taaaatataa
       61 agctgttttt agaatagttt aaatttctgt cccttttact tttttaagta accatttcta
      121 aacaaatagt tactaatttt ataattctgg tataaaacaa gtaatctcac tatttaaaat
      181 aaaatatatt tgcctaccat caactgaagc tccaaaataa ctataattag gaacagctcc
      241 tttatcaaaa gttaattttt taattccact actagaaaaa ataacaactc cacatttgcc
      301 acttccagct ttataaccat aatctgatac atataaagtt gaacccaatt gtacagcaaa
      361 agtattacta tcaaagcctt ctcctgcata actataagtt ccacttacag ggtcgtaatt
      421 aattgttgaa aaatagtgag aagaaataga actttttagc ccaaacacaa caattttata
      481 agtgctgact ttagcgcaaa caaaataatg cgattgtttt gaatctatta tagttgattt
      541 tgtagaccaa gtgtttaatg ttgtattata atattcaaca actctgactg cttgagatga
      601 agaagaatct tctccgccat acaaaacatg tatttgattt gaatacattt cacaacaagc
      661 acttcctctt gcggtaggca tatttgctat ttcagtccaa gaactagtta aaatattata
      721 tttttgacat atagctgtgg gagctgaata tccactaaaa ccacctattt tatagatata
      781 tccatcataa aaaaatagcg ctgattctac aaaggaagaa ggaatatttt tagttgttgt
      841 tgtgtttaaa acagtatcat agcaaagatt atttaaacct gatgtacctg tataaccgtt
      901 tataaagtaa atcttttttt ctgccgaaac acatggtgtt ttatctttaa ccgcagtaga
      961 caaagcttta ataaatatat ttttaggaac ataccaaggt ggaacaatca tgttgccttg
     1021 ctctatttta ccaacatttt cagctaacgt tttaaaaggt gcattactca cagttgatac
     1081 acctttttta cttagatttg aaacaaaagt acttcgtatt gaacttaatg tattcttagt
     1141 tgtttctaat ttgtcatttc ctgtgaaagg caaacctaga acatcagcta tattattttt
     1201 gactgtctgc aaatcatctt tcgtggtttc taatgtatct atcacttctc ttagttttgc
     1261 attttcttct aatttcgttg ccatttttca ctctccttta ttaaatcata tctattaaat
     1321 tattcactat agttattcct ttagttcttt gaccacttat ttctaccatt atttcctcta
     1381 atactccatc taacttatca cttgtaaatc tatcattagc atctgtaata cttatactgg
     1441 tatctataag ctgtatactg ctaatagaat cctctatttt cttagacgaa taagtagtca
     1501 tttcagacac tctgttatca tccacagttg cattaataaa atgagtttct gcatttccat
     1561 ttatcacata aacgtttaat tctgaccttg cttcacttct aatttctata gaattatcat
     1621 ctataatttt aaagtttgta actacatttt cttttgtagt agcatctata atatttacaa
     1681 ctattctctg tgttaataaa ctatgtgtta cagttgcttt gaatccactt tctgcatcct
     1741 caacccaatc atcaattgcg accctttgag tgtatgcaac atttgagcca cctgcgatta
     1801 attggtcgat tttagtattt aactctgttt tagcagtttc tatgttgctt gttaattctg
     1861 ttttagttgt atcaatttta gtattaacag tacctatttt agtttctaag tcttgtatat
     1921 ctttgagtgt tgcaaagatt attgttgggt caattttaag ttctatatta tttacattag
     1981 atacaataag cacagtttta accttcatgt ctaccactgc accttgttct atagaaggtt
     2041 tataacactc tttgtattta gaaatggcaa ttaaattatt ttcatcatct aaatatccta
     2101 tttctcttat cataaacccg cctacacttg atggtattaa actctctaat attatacaat
     2161 ttggtgcagt ttcatctgta gttgtatttc caatattgcc ttcccatacc acgtttttga
     2221 gagctgtctg actctcagtt ggagtatatt cactccctcc tccatcacca agttgaattt
     2281 ttacaaatcc cactttatta cctgtgacac ttgcatttgc tatctttgct ttccctacat
     2341 ctgtaattat agtgtaataa cttttatcta tagccaatat atcacctcct aaaatattgt
     2401 tatctcttgg tatccaactc cattgccagt taatacatca atttctccat aagtttctat
     2461 atctggtgga ctccaagggt atatagttat ttcttgacct attagggttg ttataccaaa
     2521 attcatataa ttgtctttac ttacaagcac tctagtgtaa tctaaagtca tgttgcaagg
     2581 tttaatacta cttacaaaag aatgaacctc ctcaaaccaa tcttgatttc tagcatcact
     2641 ttcaagatgt atattataag tagcattatt aatagttaat tcataattgc cttctccaac
     2701 tatactatct agccagttcc ttaaaaatct ctctgagtaa ggtagtttac ttatatattt
     2761 actaaaaatc ctaaaccttc tatcttctaa actctcatta cttttaggag ttatagacat
     2821 tatcttttcc catcttttta taccacttgg agttaaatcc tctaaaaact ggtcacttga
     2881 taggtcattt aatttttcat gtagtgtttt tatttctttg ttttctacat taaatacttt
     2941 tatatattct tctttatctt gtagaatttg tggtaagtaa tttattagat taatctcttt
     3001 atccaactac ctcacctctc actactatac tgttactatc tattgttaga ttagatttaa
     3061 cctcatttat cattgtattt gcaatgtcta atactccatc aatactaagt aatctagttt
     3121 caatttgaga tatacggact attaagtttt cttcatcttc ccaactcatg ttaagttcat
     3181 ttaaatagtc gtctattgct tcttctgcaa ttgattttat attctcccaa gtgtagccat
     3241 ttttgtatgt tatctctgct gatatattta tagttgtact tgtaactcct tcaactgtga
     3301 ctcggtgtcc aattggtgct aatccaagac cttctccttg gttttgtaga ggatcaattt
     3361 cttcttgaac taaattaact aaatcactag atggaacttt gaagttagaa ttaattatta
     3421 ctaacttaac agtacctcca ccgtcccaca caggataaac cttaactcct ccaacatctt
     3481 gtattttgtt aacttcatct ttataatttt gcatatttcc accaaaactc tgtgagttta
     3541 ggctatcata atatctttgt cttaaactat cttcactttc ttcatcttct ccattaatta
     3601 gtatttcagt taactcagca gtttctaatt tgtctacata ttcaattgga atcaactgac
     3661 caagttcaaa tacaggtcca gtagtttcac atttcatttt atatgttttt tcagatattc
     3721 tctcaattgc cacataattg tattctccta agttgaatct tgaatcaata ggaatatcta
     3781 tattaaaaac tccttttgca attgtattgg ttgcaggtaa aggtgtaata cctctttctt
     3841 tacatctctt ctctaaatag taataactag cagtatctac aaaagtttgg tctagtaatt
     3901 catccatggc aatgtatgtt tctgtaagtt ctatagcaac aggagcaaga gcattatata
     3961 ttatagaacc ttccctttta tcaaaagtat ctggtacact atctaacatt cttttaatta
     4021 tattttcaaa tgtcattaac tcaaacaatt atacactcac caccttctct gcttttatat
     4081 ttccatattt actgtaaact gtgaacttac aatgtacttt accctttaca ttttgaaact
     4141 caaaattatc tacattttca accctatcat cttgaattag tgcttctttg attcgtcttt
     4201 caagttcggg gattacaaag gatataggct ctccaataag gtcgttcaac tcgactccat
     4261 aattccaact atatattaga tgttggtatc tctctgtgtt taaaattaaa aagatggttt
     4321 gttttaatgc ttcaacatca tcacaaatac catctatctt agatttttct atatttaatt
     4381 taaaggtctt acttggttct tgccttacat caaaattaat tattgataca tcttcaatgt
     4441 cataatctaa attatcgctt ggtaacactt catcacatcc tatccaatac taaatattgt
     4501 tgtcctcctt gcatacgaat taagactact ttatctccta tttttttatc tgtatatctt
     4561 ttaaatgtat ctgtttgtat tagaaaaatt tcaccaatag atagtttttg ttctatctta
     4621 actcttagag gattaatact ttctattgtt ccaaatacaa ccctcattgg gttgcttgtt
     4681 tctactgcat ccattgcagc tttttttatt atctgtaata aatcttggct catattgtca
     4741 cctcacttat ataaatcttc tcacatgtgt gtatgctttt ccttttctat aagaattaac
     4801 agactctatt tttaccacat ctcctgtttg tggtgaatga attatttgat tgtttccaat
     4861 atacattaca acatgattac tacttcctcc accaattcta cataataagt ctcctgcttt
     4921 ccacttgctt ctatctttta aatctactgc ttttcctgct ttactttgtg cagaagcggt
     4981 acgaggaatt tttataccta tttgtttata acaccattga gtaaacccac tgcaatcaaa
     5041 attattagga ccttctgctc catacacata attacaaccc agtttacttt ttgctatact
     5101 aattaattta tcttctttag agttattatt tgtactactt tggttattac cttcaacttg
     5161 atatgtttgt tcttcatctc cacctataat tatatagcca ttctttctac caaatttttt
     5221 acattcacta gcattagcta atagtatatc tatatgatat gttccgtttg tttcaacata
     5281 tattcttcct ccattatctt taactgtata tactttgttg tcataggcag taccaggaag
     5341 tataattttt actttatctc catattcaaa aactggatgt ttctttagaa aatcatcagt
     5401 ataccaagtt ttcttaactc cttctcgatt cattggacca gcaacagttc ttgattttac
     5461 atcaagtggc tttccattgc aatctgtttt tccgccttcc attgcattgt tccctggata
     5521 atatgcagta aatatagcag gaactttttt acctgtattt tttttcgtaa tactttgtgc
     5581 aggaccattt ttcttttcat ctttattatt agtatttcca ctactatatg agcttgaaga
     5641 atatgaagca aattcgtctc catcaacaag ggtcaaatcc ataaagtgcg aattattttc
     5701 aaatgtatgt tttactttct caactaacat ataattttgt aattcaatat ctcccaaatc
     5761 taaaaaaaca ggtactaaac aacctgctct cactctaata tcaccaagta cattttttaa
     5821 acttaatgac ttagttttct tattatatag ttttagaagt atatcacact tttgttttat
     5881 ctctgcttca ctcatgtttt tgtctactgt atcaaacatt tgaagtattc cccaactcct
     5941 catatgcgtt gagtcttgag caatatacac atctcttttt cctgtttctt cattatctct
     6001 cacaagttta atctttgtgt aagtatcact atctattgat gaattatagt caaaatcttc
     6061 tattacatca ttgttcataa cagtatctaa tttcattgat gcaacattct ttaatgttat
     6121 tcttccaaaa tcatcatata acacatacat ttcttttttc tctcttagag tatcatctag
     6181 tgctgttagt atcatgtcaa agagtgtttt attttcttcg actctagata gtttatactt
     6241 agtatcttct atgacattgt attttaaatt aaaatcttta gccaacattt ttacaagttc
     6301 acttgctgtt ttattgctat acacataagt atctttgttc ttaaaatatc ttaactggtc
     6361 gtaagcaaca attttaatgt gattttcttt atctcttttc ttctgaaata tatatccata
     6421 gaatatacct acacctttgt aatatagtct tacagagtta ccttcacaaa actctaatat
     6481 atcatccatg actattgtaa attcaagctt agatggtgtt cctcgtctct ctatttccca
     6541 tgtgatacca tcaatgacag caggttcata gaaatcttcc caatgtgcta tgattaatct
     6601 tacatctcta tcattcgcca gaactaaatc atcagccaag ttttaacacc tgccctttgt
     6661 aaatagtgta tttacttaag tttttgccct tatttgcctt atccatcata gatttattta
     6721 gttcgtatac tttcttatat aatgaaccat taccaagttg cttcttgcag attaaccaaa
     6781 ggctatcccc tgcttttact gtatatgttt taccatttgg cttattggat gaatctggtc
     6841 taaattcttt tggtttcatg actggagggg gagtcctacc atagtttgtc ttttcaggag
     6901 ttgcaggaac taacttttta gttgagtaat ctctatattg ctttaacttt attgcaactt
     6961 ttgtatctga gccattttct gcatcttcta ctatgttata ttcctcaaga gatactttca
     7021 tattagtgtt aaatagtact ttattaccta attccctcga tacaataaat tgaaatggct
     7081 tacaatcagt ttttagtaat tctagtttac ttaaaaagaa ttgaacatct ctaaattgac
     7141 ctctacaaaa aggtaattta ttatgtgtaa attctgcttc aaaacttatt tcagataatc
     7201 cttctttttt taatatattt acttctccaa catttattaa atcaactgtc ttgtttttat
     7261 ttgtaacttt aatctctaac tttggcggag gtattggtaa ttgtactcca tctaaataaa
     7321 agtcataagc catttatatc cctcctctct aaactattcc ctcagctgat acaaccatag
     7381 cgtcgtttaa tttttctgtt agtacattta ctattccatc tacatctgcc tcactattta
     7441 tgttgtttgt attgttcatg tcaattttaa tgtttactcc tgtgaatcta tttattgttt
     7501 cttgctctgc aatatctcta agatatttta agtcttcttg acttttatcc attgttttag
     7561 ccattttggc agtatttccc gccgtgtcct ttacattttt tcctaaatct ccaccaaatc
     7621 ctccaagatt attaagtgga ttgtctttat cccacaaatc ttttaatcct agcttatctt
     7681 ttgcatcttc tagcatctta ttaatgtcaa aagtatcttt taatttatta gatatagcat
     7741 tttgccattt agttccaagt gcatttcctt tttgaaattc tgctccaata tctttgtatc
     7801 ccattcgctc caattttact ttctctggtg cgtctcctac ccatctgttt aagctatcaa
     7861 tctgttgttt gatgtaacta ttatctgcct taactggcgt aaacgttgcc tctcctactt
     7921 taccaatatt tatccctggt attttattta ttaggtcaat taacttattt acccctctta
     7981 ttgctatatt tgctccatcc acaaaagcct taccaagtgc gtttcctgca ttatttacag
     8041 aatcattcaa tgatgccatt ttctcaatta taaaaattac accttttgca attgcttgtt
     8101 tcatcaaata tacacactga ttccatccat tcgcaattcc ttcatttaca tctacacatc
     8161 tattaaatag ccatatcatt atattttgta ttgttgcaac tgctgcaaat actgcaccta
     8221 caatcacccc tagaacagtc aaagacgtac ctgcaaattt atttattgcc gctactacca
     8281 taaatattac agctattact gctataaatc caagaactat ccacacgagc gggcaagcat
     8341 acaacgctgc atttaggcca tattgtgctg ctgttgcctg ggctgttacc atcgcttcta
     8401 tagtttgtgc agatatttta ccaaataaag ctaattcttg tgctgccaat gctgcacaag
     8461 ctttatatgc ccctaaagca tacagggcat ttacaaaaga tgatactcca gttacaattg
     8521 cacttgcaag taaaattcct ttatatattg caatagctgc gacaactcca taaacagcag
     8581 gtgcaataat actccaattt tgagcaaaga cattagcaac atttagtgca gcattagtaa
     8641 tccatcccaa tccttgtatt atcagtacag ttcctgttgt aattccttgt acaaaatctg
     8701 aaaaaaaatc actatttaat atatttttta taatatttaa tgtatcataa atgtttgaag
     8761 atattgcaat cataacattc cgcatagata caacaatctc actaaaacgt acactttgca
     8821 aagctccgct tatcttactg agagtctcag aaaatatcat ataagcatca tttttcatca
     8881 ttgtgagagc ttgagaaaaa gtgattggca tacttgcaaa tttcttttct atctcatcag
     8941 aagctttaaa cagtgcattt cttataacat ctgctgttat tgctccgcta cttgataatt
     9001 ccttcaattg gtctttagtt tttcccattg catcagctat tttagtggct aataagggtg
     9061 cattttccat tatggaacgg aactcatcac cttgaagttt tcctgcaccc atggcttgtg
     9121 ttaattgata cattgctgca ctagcttcat ttgctgatgt tcctccaatt acaaaagctt
     9181 tattcataag ttctgtaaac tttatcacct ctgctgaact tccaaaagca tctcctgcaa
     9241 gtattccaag tttagcaatc tgtgctgctg tatctgcata actagctctt gcactttggg
     9301 ctgacaaata aatcatttta ttaagctggt ctgttgtctg taagccatca ttcattagat
     9361 ttaaacgtgc ttttgtactt gcaattgtat ctgctgcttt tgtaatactt tctattcctt
     9421 ttattccaat gtatatccca gcaatacttt ttaatttaga cactaataga gaacctgctt
     9481 cactaccttg ccttatttta ttattaaagt tttcctgctc atttgtattt cttccaattt
     9541 tctgttctat ccttgtgaga atactttcta tgttattcaa actttgttga gaagcttgta
     9601 tattacctgc attgagtgga ttattcaatc tgccttgcaa tctctctaaa ctattaattg
     9661 ttgtattaat agaattagtc atattacgaa atgctggtgt cattccgtcg aaaatcttta
     9721 ttgaagtttg tattgtagcc atttcctcac tctcctttca taaatttcca tataaaaaaa
     9781 cacctacatt agtaagtgtt tattatttat gcccaaaact gtttaccaca gctcaaacat
     9841 gtaactctaa ctttctttgc tcctaaattt ccagctacta aaccaatgcc tcctgctata
     9901 gtagcccctg ctacagcttt tcctatgcca aaacctttct tatgagcaga caatgaaaca
     9961 gagccacacc tagggcaacg agcaacatca tcttcaagta cattagtttg tttatactcc
    10021 tcttccctca atatctcttt ttcctcttcg atatttattt tttcacttaa ttctttaaaa
    10081 gctggatata taatattttt acactctttt atgtccagcc ctagtaactt cattgcaata
    10141 tcaattgcta agattttatc tcctttagtt tctttataaa tattagtcag atttaattct
    10201 tttccatgaa catttataaa taaatcatca ttgttaactt tatctgctaa tgtagctgtt
    10261 ccgcaatttg agcaaaattt gcctgcacca gttatctcat aaccacaatt tgaacaaaac
    10321 acaacaatca cctcacataa tacttatttt atagcactat tatactatat acgtaaaatt
    10381 tttacattat tatcacctcc tttcataaaa aaaaacactt actcatttgt aagtgttttt
    10441 gaattatttt taattttaag tccacatagt taatataaaa cgagcatcat ttatcattgc
    10501 taatcgtgca tttgtacatt tacataccac atagttaata taaaaccata cactagattc
    10561 taccccaaaa atgaagctca atatatttac ataccactta gttaatataa aacgcgttaa
    10621 tgcagtacca taacgatatg ggactaacta atttacatac cacatagtta atataaaacc
    10681 gtataggaat gaatgcaggt agtgcatttt ctagtacatt tacataccac atagttaata
    10741 taaaacgcaa cccctaaaga ccttctaaaa aaattactct tagatttaca taccacatag
    10801 ttaatataaa acacaggtgt atttacctat agaacaacaa gagttacatt tacataccac
    10861 ttagttaata taaaacattc aagaagtaac tgtaattgtt catctgtaaa ataatttaca
    10921 ttccatatag ttaatctaaa acctggtctt aaaaacatac atgttgcaaa agtgaatgat
    10981 gaatttacat tccatatagt taatctaaaa cgaacactaa caaaaaagct aaaagtggtc
    11041 actatattat ttacattcca tatagttaat ctaaaacgaa tgatacatta gaatatatat
    11101 tttgtagtga atgatttaca taccacttag ttaatataaa accatcctac ttagatactg
    11161 cacaaatagt atcgcgaatc tttacatacc acttagttaa tataaaattg accagtacga
    11221 atgcgaggct agcaatgata aacgacttta cataccacat tgttaatata aaaaccttga
    11281 gtgacgcagc aaaaggggca aaggatacag catttacata ccacttagtt aatataactc
    11341 ctacctatat tatatcattt tttactacat aaagcacttg aaacagtaaa atattcaagt
    11401 gctttatttg tctatttatt tttctctttt tttcctacat tcttctttca ctaaacttac
    11461 aaatctataa aattcatctg gattttcatt tttcattttt tcaaagatat ttccaagttc
    11521 tttgataagt tctattctat ctatatcaag taaattctca tgcattgtat taatcccccc
    11581 aaaactaaac taaaatattt aatacaactg ataaatttac tcaattttat aaaccacatg
    11641 ataattcttc ttctcacctg caatcttggt aggtctatta ttctcctcta tccaatttct
    11701 tattttatct attacacttt ctgtatactt atttacagta ccagtccaac ttccattagt
    11761 ctcccaaact cccttaactt cactatcttc taaatcaatc tttttaataa tctcacaaac
    11821 agccatctga gctggtttat tactcttaga atatagtttt aacttagatg ctatttgttt
    11881 tgtgtcaaaa taatgttctt cttcgtttat ctctattggc aaatcaattc ctgccttttt
    11941 atataatgtt ttagctgtaa gtaacttaga tttattgtca aaacctgcac catccaatag
    12001 ttcttttaac atagatgtgc tgttataagc cagttgtaat ttttcaatct cacttgcttt
    12061 ttctcttagt ttttcggggt cagcattatt tgttatgtat gcaccagttt gtcgaatggc
    12121 tggaagtact tcatcactta tccaatcttg aaatctctca gcttcttctt ttttagattt
    12181 aaatataagt ttataaacac cactttcagt tagaaaattt tctcctgtat tatgcaattt
    12241 tctaaactgc atattatgca ttttagaatt agtcaactta actacttgat tatcattaaa
    12301 ttttctaata ctactgttca catctgaaat atctaaacac tctgccacat gctttggatt
    12361 aaataaaatt tgcccttcaa attcgaatac ctctatatct tttccttcaa aattcattaa
    12421 ttcattcata ataaattcct ccttaaattt gattgtaaga agtaccttac tatgatagaa
    12481 tatatttcat agaaggtaac ttctttggga aacagtcgca agtgctttgg tcggtgcagc
    12541 ggctgttttt tatttgtttt tgtcaatctt ctctttcacc aattcaattc ctctaattac
    12601 aacatctgtt ttagatattt taagattttc agcacactca tttaatatat ctgcttgttc
    12661 ttggttaagt ctaacttcaa atcttaattt tttggaattt tcttttggtg gtctgcctaa
    12721 tttattggac atcttatcac ctctcttttt attgtccgta cttaaattat aatatagtac
    12781 gtacaataat tcaagagttt tttactaatt ttttctaatt attttactca accgaccaat
    12841 tttgagcaaa acaaaagcac ctaccaaaaa gtaagtgctt tctatataac agatttaatt
    12901 ttaaaataaa gtattcacct aatttccaga cgagaaatcc aaatcatcaa ttaaatttac
    12961 attatttttt atattattcc aatcatctaa aaatacatta taagtgagtg tcacatcaac
    13021 aataaagctt attctcactg aatggatatc attcatatct tttatattgt ttacaaattc
    13081 tttagtaaag tttctacact cattttcatc attaaaccta gaattttcca cttgaatact
    13141 aactatatat ccctcacctt ttgttgggtt taatatgtta acataatatg ttgttttatc
    13201 ttttaagtct tctggtatca tagagtctac ttttgcttgc atctcttgtt tataaatatc
    13261 ttctttttct ctttcgctaa ttatattttc ttttgtaata tttccttcat ttacttcatc
    13321 tctatttttt gaatctcgta tcgcttgatg aatcatcatt gatactgtaa atacaacaaa
    13381 gtatattaaa aatatcacta aaattttctt caacaaactt aattttttaa attttcccca
    13441 cataatgaac tctcccctat taaattcttt attcaaatat aattctatct aaaaataaga
    13501 acttagtcta ttgatttaaa atattttgag caaaataaaa gcacctacca aaaataagtg
    13561 cttctttctt ctatttagtt tttctccaca ttgttaatat aaaagtttgg agttttaaaa
    13621 ttatcattcc tatcttttgc tttatttaca taccacatag ttaatataaa attgaggtag
    13681 aaaatgataa aaagactttt agagtaatct ttacatacca catagttaat ataaaacctg
    13741 ttaatgtaat catttcacca tcataagttg cgaaatttac attccatata gttaatctaa
    13801 aacaataatt ggtgatggta caggatatac aaatgcaaca tttacattcc atatagttaa
    13861 tctaaaacag aaaattcatt gtacaattct tctttgtatt ttttaattta cattccatat
    13921 agttaatcta aaaccccaaa ataaacttag tatttccaat atctacacat acacacctct
    13981 ctcaaatttg cagtgaacca tgagtagtgc aattgataac atttatcaca caccctcaat
    14041 accttgtatt ccaattgtta aacttcaatt tatcgcaaat attgctcact gcaaaatctc
    14101 tacattttta ttatatcata aaaatattat ttttgaatat ctgtaccaat ttgtggtata
    14161 ataaaagcaa ggaaataatt tactttatac aagagtagct atttccatca aaattgattt
    14221 aaagaattat ttttttaaat cacccttatt ggcgtctggg tgatttttta ttttgtcata
    14281 aatataagct gatataacac cagctagtat gcttaataaa aaaactatca tataatttca
    14341 cctccttcct tatttggaat ttggcgttta atatgaaaat aatcaccctt cgcactttcg
    14401 attattatcc ttgctacaat tattataaca tataattatt acatattttt ccattttttt
    14461 tatataaaca atgaaat
//
LOCUS       DAMJYR010000094        14165 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.097, whole genome shotgun sequence.
ACCESSION   DAMJYR010000094 DAMJYR010000000
VERSION     DAMJYR010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14165)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14165)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14165
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.097"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            286..516
                     /locus_tag="PC471_002725"
     CDS             286..516
                     /locus_tag="PC471_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2789839.1"
                     /translation="MRLDMDSMLYVLIYMTLLVSIIGSIFYFCKDLLNHKIKYVLIRD
                     IFVNRITYSEERLPKQYIVFQKYDIWRYCSLF"
     gene            573..1223
                     /locus_tag="PC471_002726"
     CDS             573..1223
                     /locus_tag="PC471_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HDF2789840.1"
                     /translation="MDFISSILQGLLSILFNFTGDFGISIVLITLIVKLVLMPLSLKQ
                     RFSVKKQQELAEKMEHIKEKYKNDTKELEKQIQVSSVESMKSMLGCLIVLVQMPVVYA
                     LYHVCLDMTKEFTTIIIPWIANLGTFDNLFILPCIYTLIMLASNLVNYIPYLKINSQV
                     KFNKQMAVVTTITSLILTVRTPVAIGLYFITSAIYSLIEDICFRIYFNRKNRIVTK"
     gene            1437..2108
                     /locus_tag="PC471_002727"
     CDS             1437..2108
                     /locus_tag="PC471_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2789841.1"
                     /translation="MAKILIVEDEKRMQSIIVEYMQKGGYTCITADDGVEALTILKSN
                     NIDLMILDIMMPYLDGFSVCRVSREMTNIPIIILTAKGEEEDKLKGYEYGADDYITKP
                     FSPKVLLAKVNALLRRYTTDIPKNSLSLGKIFIMVASRQVYVEDKLIDLTYKEFELLR
                     LFMENPNQVFSREKLLNCIWGYDFEGNTRTVDTHIKTLRKKLGSEGHHIVTLIRSGYK
                     FEVKE"
     gene            2105..3235
                     /locus_tag="PC471_002728"
     CDS             2105..3235
                     /locus_tag="PC471_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF2789842.1"
                     /translation="MNSLRKIIEKLKNVDISNWIKQNFGLYSVRKKVFLLSKLSGALI
                     ILFYLVVEEFSINSKLEFWIWFIILVIFIMSIDFLLGKFISEPITSINKSAKSMSQLD
                     FSNPCTVNTNDEFGELSRSLNTMSTNLQQALSDLESANIQLEKDVNKERMLLEQRKEL
                     VDTISHEMKTPLGIIRAYTEGLMDEVDEEKKKNYMNVIIEETDRMNNMIVSLLDLSAL
                     EAGVSKLNPERFDFIEFVETVAGRLLIDIPDANFYFTYDLPEYEVFVVADKMRMEQVV
                     ENLVINAKKHVVYNGNLDLSITCENGLLLFEIYNDGRFIEQDEISKIWSKFYRSVQSQ
                     RTGGSGLGLAIVSQILTMQGLEYGVNNRDRGVEFYFMIPIDE"
     gene            3655..4500
                     /locus_tag="PC471_002729"
     CDS             3655..4500
                     /locus_tag="PC471_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2789843.1"
                     /translation="MIKVGELKFSYGKDKQILHGLNFEVKEGEIFGFLGPNGSGKSTT
                     QKILNGVLKGYNGHVSLFGKEVKAYTESLYQKIGVLFEFPYLYTNLSAIDNLEYFSSF
                     YPKNQRRDIYELLDLLEFKKEFINKPVSSYSKGMKQRISMARALVSNPKLLFLDEPTS
                     GLDPSGAVLFRKIIEEERKKGTTIFLTTHNMLDADLMCNNVAFIADGKIMVIDKPKNL
                     KMKNSNNKVEVEFVYNGNRDIEIVDIQELESGITFKYDEILSVHSKEPTLEEVFIKYT
                     GRMLV"
     gene            4500..5528
                     /locus_tag="PC471_002730"
     CDS             4500..5528
                     /locus_tag="PC471_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2789844.1"
                     /translation="MYSKFFTLVKKDFWMMIYGKFFVLTLGSLILYSCYINFGYVKFM
                     DEDSYHNNVYLYNPENISMSASPLINQVSSIGELKAKLDEDTSAVCIDMSSSKPKVIL
                     HESIKGVDDYRADYALSLLSSEKDHNSKISDNNAIIQINDKDTSEIKQRKDMTCEILF
                     FEIVAIGFLGIASLLFKEKQMGVMRITGILPVKRSLFILSKIFAFLICDLCFTVLLVV
                     FNIGFFEGIKILPQVLVQASILSIIMSLIGIGCSMLLRDFKQFSLIYLVVAVFITTPI
                     FLSANTSFEIDWIKYHPFYHVYSQLKNAFFMNFSKELVYFVVCSLTIIVLFLLVKYIF
                     NKELVREG"
     gene            5531..6544
                     /locus_tag="PC471_002731"
     CDS             5531..6544
                     /locus_tag="PC471_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2789845.1"
                     /translation="MIGLRYQLKSIKKDKMCIISFFLPVVMAILINFMGTIDISSLGE
                     IRFGIVKDTLPVQTEQWLKKYGMVSVYDTKMHLIREIKDIKTDTIGVVRDGKGIKTVL
                     YGNEINAIKNVGKTLPEMFRLKGDLKDVEVTILPKDSILKEFQNVFIAMILITAMFMG
                     CTFNAMNIISEKEDGISFINEVLPQSRSQYIIQKVFLGLLCGCLSSMITVFICLDISI
                     LDMIMMLLLVVFSAFISSLIGLFIGKLSEGLMVGIVYIKIVMILFIGTPLLIYLLGVN
                     TSGIGNLYYIMPSVATFNGIMEIINGNVIIIKDIVILAVHCVVWFMIYIILDKKRKLF
                     FSR"
     gene            complement(7061..8311)
                     /gene="cme"
                     /locus_tag="PC471_002732"
     CDS             complement(7061..8311)
                     /gene="cme"
                     /locus_tag="PC471_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter Cme"
                     /protein_id="HDF2789846.1"
                     /translation="MENKNTNSTWKKKVILFLASQYISLFGSSLVQMAMIWYVTLQTS
                     SGVWVTVLTVCSFIPQMIISFFAGVWADYYNRKVLIIVSDSTIAISTLVLILFMMSSN
                     GTTSELIAISVISVIRSLGSGVQTPAVNAMIPQLVPEEHLMRFNGINSSLQSIVQFIV
                     PIVAAAILGFGNKINQVLLIDVFTAIIAIVLLIFIKIPKHDIPKQTEKVSFFADMVSG
                     IKYCFSDKFIRKVLFTYGAFMLLCVPSGFLIGLLIERTFGKGYIYLSIIEMIGFLGMV
                     LGGLIIGVFGGFKNRNKTFFLAILSYAIFSIILGLVTQAWQFFIFMFFTNFSIPIIQA
                     SAMTMLQEHVAPQMLGRVLSLPIIIYTGFIPLGMMIFGPMADVISINNLIIIAGIILL
                     FFALAIPYSKQFYKQGISKSNESE"
     gene            complement(8397..9143)
                     /locus_tag="PC471_002733"
     CDS             complement(8397..9143)
                     /locus_tag="PC471_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDF2789847.1"
                     /translation="MDTYTTSEVADLIGVHPNTVRLYEELNLIPKPKRNTNGYRIFND
                     FHIEQFRFARTALKIEVVQRGLRKDALEIVRTSANRDFDKAISLVNQYLQQLEIEKSN
                     AEEALTIVAQILNGNTPETNKLFLTRKETAEHLNVTIDTLRNWEMNGLLKVKRKQNGY
                     RVYTDEDIKRLKIISALRCANYSLSAILRMLNALSSNPNTNIKTVLDTPKDDDEIISV
                     CDKLITSLKEAEHNAKTMKSHLQIMKKQFS"
     gene            complement(9258..10280)
                     /locus_tag="PC471_002734"
     CDS             complement(9258..10280)
                     /locus_tag="PC471_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HDF2789848.1"
                     /translation="MNKNNCLDLIFEKVNPSSVGMSSEKLSQLEPLIISQYSNINGII
                     IARKGKVVFEKYYNGYNADDTFHVASVTKSIISALIGIAIDKGYIKDVDQKVVDFFPE
                     YTYSPSEICKKAVTIRHLLTMTAPYPFKNMTESLERLCKQKDWIKYTLNQLGQKGEIG
                     TFKYSSAGTHLLSAIITKTTGKSAREFANEYLFKPIGMKVIPDYDMKDFGFEDFFGKN
                     LKGWAKDRQGYSIGGWGLTLSARDMLRFGFLYLNDGIWNNKRIISKSWIEESVKMNSN
                     EYGYLWWLCKKDGVLWYSAMGAGGNAICCIPQYDLVIAIPSKIVFKPRDRWEFIEKYI
                     LTSIID"
     gene            complement(10280..12700)
                     /locus_tag="PC471_002735"
     CDS             complement(10280..12700)
                     /locus_tag="PC471_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HDF2789849.1"
                     /translation="MKEIKINGAKIHNLKNIDVSIPKDKLVVATGVSGSGKSSLMFDI
                     VFEEGRKQYLQSLGILAGIDSEDKFDNIQGLAPTIAVQQNIIRQSNPRSTVGTRTNIL
                     NMLTLLYSVEGQIMCTMCDTPVSYNLICNNCGYEEERLRPSYFSYNSSDGMCMKCSGH
                     GAYFEINIEKLVLDKHDTLKQVLDRAKITPGYLRVFSKKFNDYLDIQFLQLPEEVRNE
                     VLYGHYENGKKSSSLSKVFHNRYKRGEDLNGVYTMTACSDCNGFKIGEEARRVLLNGK
                     HIGELGKMTILEIDDFLKLLINQGNLNTFGTNLVNDILSKTQHLIESRLGHLSLYREM
                     STLSGGEMQRLFLNSHLDSEMDSLIYVLDEPTAGLHESEKIDILKSIKKLKDLGNTVI
                     VVEHDKNTIKMAEHIIDIGPKAGVEGGQLVYQGDLEGLLQSDKSLTGQYLSGKYPMPN
                     RTSNKNITHTDKIPCITIQNANTNNLKNVTVSLPLGAMVGISGKSGSGKSSLISDTLL
                     PLLRSQFNNHTINNQINSSESEIGEEDDYAVVETIADRLIGTKHISGYSEISQSPIGK
                     NMNSTPASYIGIWDKIRTLFAGQPESLERGFTAGHFSFNSKGACPKCSGSGYEKVWLG
                     NNLSIDHICSECQGKRFNDESLSIKYKNKNIHDILNMSVSESVHFFKDIPNIVSHLNV
                     LEQIGMGYIKLGQPTPTLSGGESQRIKLAKEIGKKRNGNILYVLDEPTTGLSLYDTSK
                     LIQLLDELVENGNSVIVVEHDIDVLNVCDWIVELGPEGGDKGGYIIAEGSPKTLKENP
                     KSITGRYL"
     gene            complement(12786..13706)
                     /locus_tag="PC471_002736"
     CDS             complement(12786..13706)
                     /locus_tag="PC471_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HDF2789850.1"
                     /translation="MTNAKSKKEYLNRICKVQDYIEEHLHEPLSLDELSNIAGFSKFH
                     FHRIFKAIEKETLAQYVNRLKLENATAFLIHRTDMTVTDIAHYFGFTDSAVFSRAFKN
                     YYKISPIKYRNNYSKNCKEPYKISQYNRSISKPKCKNTREVKGEIEILELDNMNTIYT
                     RYTGSYGNLTSTFPKLLERLFTYASEQNLLGLENTQILAIYHDNPQFTQEHQLRTSIC
                     MTIPDNIVVKENDNLGNMIIPSGKYLVGHFDISKSEYSDAWDFMYNEWLSNGNYKLRA
                     SFPFEVYLNNPHNHPQNRHLVDIYLPIEPF"
BASE COUNT         5027 a         1821 c         2218 g         5099 t
ORIGIN      
        1 taaattaagg actataattt gaaaataagc tataaaaact caatattgtt ttgttcaaag
       61 ataaaaaaag aatactttag gtgattttta ttaaaaatta agagtgataa gattatatac
      121 tttataaatg taaatatgac ggataaaaat gataaatata ataatatata tatttacatt
      181 aggagttttc tatactataa ttatttaaaa ctaaatataa atgttggaat tatttaggac
      241 ttgaaattgt ctaaaaataa atataagtga tgaagaggtg atgtgatgag attagatatg
      301 gatagtatgt tatatgtact aatttatatg acattattgg tatctatcat tggaagtata
      361 ttttattttt gtaaagacct cttaaatcat aaaattaagt atgttttaat aagagacata
      421 tttgtaaata gaattacata ttctgaagaa cggttgccta aacagtatat agtttttcag
      481 aaatatgata tttggaggta ttgtagtttg ttttaatttg aaatgaatct attataaatt
      541 ctaaagttta aaaataaaaa ggagagaata aaatggattt catttcaagt atattgcaag
      601 gattattaag tatattattt aattttacag gtgattttgg aatttctatt gtacttataa
      661 ctttaatagt taaattagta cttatgccac tatcattaaa acaaagattt tctgtaaaaa
      721 aacagcaaga attagctgaa aaaatggaac atataaaaga aaagtataag aatgatacta
      781 aagaattgga aaagcaaata caagtaagtt ctgtagaaag tatgaagagt atgctgggat
      841 gtcttatagt tttggttcaa atgccagtag tatatgcttt atatcatgta tgtttagata
      901 tgacaaagga atttacaaca attataattc catggattgc taatttaggt acatttgata
      961 atttgtttat attaccttgt atatatacat taattatgtt agcttcaaat ttagtgaatt
     1021 atataccata tttaaaaata aattctcaag taaagtttaa caaacaaatg gctgtggtga
     1081 ctaccataac aagtcttata ctaactgtta gaactccagt agccataggt ctgtacttta
     1141 taacaagtgc aatttattca ttaattgaag atatttgttt tagaatatat tttaatcgaa
     1201 aaaatagaat agtgacaaaa taatataatt attattagaa atattttagc ctgctaacat
     1261 agaaaaagtt agtaggctat ttttatctat gatatcaatt ccattaagaa tagtctaaaa
     1321 aatactatca aaatgctata aaaaatacta tcaaaatact ataaaaaaat agttgaaaaa
     1381 taaataaatt aatataaact aaaataaagc ctacaaaaaa gaggtgataa atagaaatgg
     1441 ctaaaatact aattgtagaa gatgaaaaaa ggatgcaaag tattattgta gaatatatgc
     1501 aaaagggagg atatacttgt ataactgctg atgatggagt cgaggcactt acaatattaa
     1561 agagcaacaa tatagattta atgatattgg atattatgat gccgtattta gatggatttt
     1621 cagtgtgtag agtttcacgt gaaatgacta atatacctat tattatattg acagctaaag
     1681 gtgaagagga ggacaaatta aagggttatg aatatggcgc agatgattat ataacaaaac
     1741 catttagccc caaagtactt ttagcaaaag ttaatgcctt gttacgtaga tacactactg
     1801 atattcccaa aaattcattg agtttaggaa agatttttat tatggtagca tcaaggcaag
     1861 tgtatgtaga agataaattg attgatttaa catataaaga atttgaatta ttgcgtttat
     1921 ttatggaaaa ccctaatcaa gttttttcca gagagaaact tttaaattgt atatggggat
     1981 atgattttga aggaaacaca cgaactgtag atacacatat aaaaacctta cgcaaaaagc
     2041 ttggtagtga aggacaccat atagttacat taattcgttc aggatataag tttgaggtta
     2101 aggaatgaat agtttaagaa agattataga aaaactgaaa aatgtagata tttcaaattg
     2161 gattaaacaa aattttggac tatattcagt tagaaaaaaa gtgtttttac tttctaaatt
     2221 atcaggagcg ttaattatat tattttatct ggttgttgaa gaattttcga taaactctaa
     2281 attagaattt tggatttggt ttataatact ggttatattt attatgagta ttgatttctt
     2341 attgggaaaa tttatttcag aaccaatcac atctataaat aaatctgcaa aaagtatgtc
     2401 ccaacttgat ttttctaatc catgtactgt aaatactaat gatgaatttg gagaactttc
     2461 taggagcctg aatacaatgt ctacaaatct acaacaggca ttatcagacc tagaaagtgc
     2521 aaatattcaa cttgaaaagg atgtaaataa ggaaagaatg ctattggaac aaagaaagga
     2581 gttggtagat acaatttcac atgaaatgaa gactccactt ggcattatac gtgcgtatac
     2641 agaggggctt atggatgaag ttgatgaaga aaaaaagaaa aattatatga atgttataat
     2701 agaagaaact gatagaatga acaatatgat tgtatcatta ttagacttat ctgcattgga
     2761 agcaggagtt tctaaactta acccagagcg ttttgatttt atagagtttg ttgaaactgt
     2821 ggcaggtcgt cttttaattg atataccaga tgccaatttt tattttacat atgatttgcc
     2881 agagtatgaa gtttttgtag tggcagataa aatgagaatg gaacaagtag tagagaatct
     2941 agttattaat gcaaagaagc atgttgtata taatggtaat ttagatttat cgattacatg
     3001 tgaaaatggc ttgcttttat ttgaaattta taatgatggt aggtttatag agcaagatga
     3061 gatttcaaag atttggtcta agttttatcg tagtgttcaa tctcagcgta caggaggttc
     3121 aggtctaggg cttgcaattg tttcacaaat acttacaatg caagggttag aatatggagt
     3181 gaataatcgt gatagaggag tagagtttta ttttatgatt ccaattgatg aataatttag
     3241 tattaattta taaattataa gtatctgatg taaaatattt ttctttgtat atgtaagcat
     3301 ttaaagagaa taaaataaaa aaacttaatg aaaaaagctt aatgaaaaag tctaataaaa
     3361 agcttaatga aaagtcttgt ttttctgatg aaaacaagat tttttattaa gctttttatt
     3421 atttcacttc gatttcacaa gagagacact ctgatttcac caaatagaaa taaaataggt
     3481 taaataaatt aaaaaataga agcgtaatta aataaggtta caagggggag tacttatgaa
     3541 atggtataca tggttgacta ttatattgat aatattgctt tctattccgc ttaaaattaa
     3601 atttataaaa tggtgggata gacgacaaaa aaatgataaa ggaggttatg atagatgata
     3661 aaagtaggag agttaaaatt ttcttatgga aaagataaac agatattgca tggacttaat
     3721 tttgaagtaa aagaaggaga aatctttgga ttccttggac ctaatggttc aggaaaatcc
     3781 acaacacaga aaatcttaaa tggtgtttta aaaggttaca atggtcacgt atcccttttt
     3841 ggtaaggaag ttaaagcata cacagaatct ttatatcaaa agattggtgt tttatttgaa
     3901 tttccttatt tatataccaa tttaagtgca attgataatt tagaatattt ttcgtctttt
     3961 tatcctaaaa atcaaaggag agatatatat gaactcttag atttgttaga gtttaagaaa
     4021 gagtttataa ataagcctgt atcatcctac tcaaaaggta tgaagcaacg tattagtatg
     4081 gcaagagcat tggtaagtaa tccaaagttg ttatttttag atgagcctac tagtggactt
     4141 gacccatctg gagctgtact atttcgtaag attattgaag aagaacgaaa aaaaggaaca
     4201 actatctttt tgactactca taatatgcta gatgcagatt taatgtgtaa caatgtggct
     4261 tttattgcag atggaaagat tatggtaata gataaaccca aaaatttaaa aatgaaaaat
     4321 agcaataata aagttgaagt ggaatttgta tataatggaa atcgagatat tgaaattgta
     4381 gatatacaag agttagaatc tggaataact tttaaatatg atgaaatctt aagtgtacat
     4441 tcaaaagaac ctacactaga agaagttttt attaaataca caggaaggat gttagtgtaa
     4501 tgtactctaa attttttacc ttagtaaaaa aagatttttg gatgatgatt tatggtaaat
     4561 tttttgtgct gacacttggc tctctcatac tttattcgtg ttatattaat tttggttatg
     4621 taaagtttat ggatgaagat agttatcaca ataatgtata tctatataat ccagagaata
     4681 taagcatgag tgcatctcca ttaattaatc aagtttcttc tataggtgaa ttaaaagcaa
     4741 aacttgatga agatacaagt gcagtttgta ttgatatgag ttccagtaag ccgaaagtaa
     4801 ttttacatga aagtattaaa ggagttgacg attatagagc tgactatgca ttgtcattat
     4861 tatcatcaga aaaagaccac aatagcaaaa tatcagacaa caatgcaata attcaaatta
     4921 atgataagga tacatctgag ataaaacaaa gaaaagacat gacttgtgag attttattct
     4981 ttgaaattgt agctattgga tttttaggga ttgcctcttt attatttaaa gaaaagcaga
     5041 tgggagtaat gcgtattact ggtattttac ctgtaaaaag aagtcttttt atattatcaa
     5101 agatatttgc ttttttgata tgtgacctat gttttacagt tcttttagta gtatttaata
     5161 ttggattttt tgaaggaata aaaatacttc cacaagtatt agttcaagct agtattttat
     5221 caattattat gtctttgatt ggaataggat gctcaatgtt attgagagat tttaagcaat
     5281 tttctcttat atatcttgtg gttgctgtat tcataactac acctatattt ctatctgcaa
     5341 atacatcttt tgaaatagat tggattaaat atcatccctt ctatcatgtt tatagtcagc
     5401 ttaaaaatgc gtttttcatg aatttttcaa aagaattagt ttattttgta gtgtgttctt
     5461 tgacaataat agttctattt ttacttgtaa aatatatttt taataaagag ttggttaggg
     5521 agggataatc ttgataggat tacgttacca gttaaagagt ataaaaaaag ataaaatgtg
     5581 tataatttcc ttctttttac cagttgttat ggcgatttta ataaatttta tgggaacaat
     5641 tgatatatct tcattaggag agatacgttt tggtatagtg aaggatacac ttcctgtaca
     5701 aacagagcag tggttgaaga agtatggtat ggtaagtgta tatgacacaa aaatgcattt
     5761 aatacgtgaa attaaggata ttaaaacaga cacaattgga gttgttagag atggtaaagg
     5821 gataaaaact gtgctttatg gaaatgagat taatgctata aaaaatgttg gaaagacatt
     5881 gccagagatg tttagactaa agggtgattt aaaagatgtt gaggttacaa tattgcctaa
     5941 agatagtatt ttaaaagagt ttcaaaatgt ttttattgcc atgattttaa ttactgctat
     6001 gtttatggga tgcaccttta atgcaatgaa tattatatct gaaaaagaag atggaatatc
     6061 atttataaat gaagttttgc cacagagtag aagtcagtat attatacaaa aagtattttt
     6121 gggtctttta tgtgggtgtt tatcttcaat gataactgta tttatatgtt tggatatttc
     6181 aatattagat atgataatga tgttgttact cgttgtattt tcagcattta tttcatcact
     6241 tataggttta tttattggaa agttgtcaga aggtcttatg gtaggaattg tttatattaa
     6301 aatagtaatg attttattta taggtactcc tttgttaata tatcttctag gggttaatac
     6361 cagtggtata gggaatttat actatataat gccttcagtt gctacattta atggtattat
     6421 ggagattatt aatggtaatg tcattattat caaagatatt gtaattttgg ctgtacattg
     6481 tgtagtatgg tttatgatat atattatttt agataaaaaa agaaagttat tttttagtag
     6541 ataactcagt ataattataa ttgggacaat ttaataggtg atttaatata ctttgtatag
     6601 tgtaatttat gtagtgcaat ttgtatactt taatttatgt agtgtaatag ttagttttat
     6661 tttataatta ttcaatataa tttagtaaga agttcagtac aattagagct acttagtgga
     6721 aaacttaata ggtacttaaa ctatataaat actttatttt cctccatttg tactataaat
     6781 ttaatttatg atataaatgg aggtattatt atatgatttg ataaatttca tcattataac
     6841 ctacaagtaa aggtattatt atataaagtt aataaagata gtatcaaaat attatttgct
     6901 ataaagagag gggctcaaaa taaagataaa cctatttttc aatgttgaaa tgataaaata
     6961 tatttgattt catataaaaa attttcttca taaattaaaa agtccatttt aagccccctc
     7021 atttttaacc agataaatcg agttaaacta tttaaatata ttactcggat tcatttgact
     7081 tagatattcc ttgcttgtaa aattgttttg aataggggat agccaaagca aaaaataaaa
     7141 gtataatccc tgctatgatt attaaattat taatagaaat tacatctgcc attggtccga
     7201 aaatcatcat accaagagga ataaaaccag tatatataat aataggtaag ctaagaactc
     7261 gacctagcat ttgtggtgct acatgctctt gcaacatagt catagctgat gcttgaatga
     7321 ttggaattga aaaattcgta aaaaacataa atataaaaaa ttgccaagct tgtgttacaa
     7381 gacctagaat aattgaaaaa atagcatagg ataatattgc tagaaaaaag gtcttatttc
     7441 tatttttaaa accaccaaaa actcctataa tcaatccacc taaaaccata cctaaaaatc
     7501 caatcatttc aataatagac aggtatatat aacccttgcc aaatgttcgt tcaataagaa
     7561 gtcctataag aaatcctgat ggaacacaaa gaagcatgaa tgctccataa gtaaaaagca
     7621 cttttctaat gaacttatct gaaaaacagt actttatccc tgataccata tctgcaaaaa
     7681 atgagacttt ttcagtttgt tttggtatat catgtttagg aattttgatg aatatcaata
     7741 atacaatagc aataattgca gtaaatacat caattaaaag aacttgattt attttattac
     7801 caaatccaag tattgcagca gctacaatag gaactataaa ttgtactata gattgtaggc
     7861 tactatttat accattaaaa cgcattaaat gttcttctgg tacaagttga ggaatcattg
     7921 cgtttacagc aggtgtttgt acaccacttc ctagtgaacg tataacagat attactgata
     7981 tggctattaa ttcacttgtt gtgccatttg atgacatcat aaatagaatt agaactaatg
     8041 tagatatagc aattgttgaa tctgatacaa ttatgagtac ctttcgattg tagtagtctg
     8101 cccatacacc agcaaaaaaa gaaattatca tttgaggtat aaaggagcat acagttaaaa
     8161 ctgttaccca tacacctgat gatgtctgaa gtgtgacata ccatatcatt gccatttgta
     8221 caagtgatga gccaaataag gaaatatatt gacttgctaa aaaaagaatg acttttttct
     8281 tccatgtaga attagtattt ttgttttcca tcctattcct cctttgattt gtgtgttgaa
     8341 tttttcttgc tcttaaggat aacacacaaa tcaaagtctg gagttagagt ggagggctag
     8401 gaaaattgtt ttttcataat ttgaagatga gatttcatgg tttttgcatt atgttcagct
     8461 tctttcaatg aagttatcag tttgtcacac actgagataa tttcgtcatc atcttttgga
     8521 gtgtcaagga cagttttaat attagtatta ggattagaag ataaagcatt taacattcgt
     8581 aaaatagctg aaagagaata gtttgcacaa cgaagtgcgc ttataatttt aagtcgtttt
     8641 atatcttcat cagtatatac acgataacca ttttgttttc tttttacttt taacaaacca
     8701 ttcatttccc aatttcttaa agtatctatt gtaacgttta agtgttctgc agtttctttt
     8761 ctagttaaaa agagtttatt tgtttctgga gtatttccat ttaaaatctg tgcaacaata
     8821 gtaagtgctt cctcggcatt agatttttct atttctaatt gttgcaagta ttgatttaca
     8881 agactaatag ccttatcaaa gtccctattc gctgatgttc ttacaatctc aagggcatct
     8941 tttcttaatc ccctttgtac cacttcaatt ttcaaggctg tacgagcaaa tctaaattgt
     9001 tctatatgaa aatcattaaa gatacgataa ccattggtat tacgcttagg tttaggtatt
     9061 aagtttagtt cttcatataa acgtactgta ttaggatgta caccaattaa atctgctacc
     9121 tctgatgtcg tgtaagtgtc cattagtatt caactccttt tagatatata gtattagtca
     9181 taataacacc aaacaaaagt ctggtgttat aatggagggt ttggttatct aagagtaatt
     9241 aatttatgaa agagtaatta atctattata gaagtaagta tatatttttc aataaactcc
     9301 catctatcac gaggtttaaa cactatttta gatggtatgg ctattactaa gtcatattgt
     9361 ggaatacaac aaatagcatt tcctccagct cccattgctg aataccacaa aacaccatct
     9421 tttttacata gccaccacag ataaccgtat tcattactat tcattttaac tgattcttca
     9481 atccacgatt tagatataat acgtttatta ttccaaatac catcatttaa atacagaaaa
     9541 ccaaatcgca acatatctct agcactcaat gtaagtcccc agccacctat agaataaccc
     9601 tgtctatctt ttgcccaacc cttaagattt tttccaaaga aatcttcaaa accaaaatct
     9661 ttcatatcat aatctggtat aactttcata ccaataggtt taaataagta ttcattagca
     9721 aattcacgag cagattttcc agtagttttt gttataatag ctgaaagtag gtgcgttcca
     9781 gctgaagaat atttgaaagt accaatttcg cctttttgac caagttgatt caaggtatat
     9841 tttatccaat ctttttgttt gcaaagtctt tctaaagact cagtcatatt tttaaaaggg
     9901 tatggtgcag tcattgtaag taggtgacgt atagttactg cttttttaca tatttcagaa
     9961 ggactataag tatattcagg gaaaaaatca acaacctttt ggtcaacatc ttttatatat
    10021 cctttatcta ttgcaatacc tattaaggct gatataatac tttttgtaac tgatgcaaca
    10081 tgaaaagtat catcagcatt atatccatta tagtactttt caaagacaac ttttcctttt
    10141 cttgcaataa ttattccatt tatattacta tattgtgaga ttattaaagg ttcaagttgt
    10201 gagagttttt ctgaactcat gccaacactt gaaggattta ctttttcaaa tattaaatct
    10261 aagcaattgt ttttattcat taaagatacc tccctgttat agattttgga ttttctttta
    10321 gagtttttgg agaaccttct gcaattatat aacctccttt gtcaccacct tctggaccaa
    10381 gctcaacaat ccagtcacaa acatttaata catctatatc atgttcaaca acaattacag
    10441 aatttccatt ttcaaccaat tcatctaata attgaatcaa cttagaagta tcgtatagac
    10501 ttagaccagt agttggctca tccaatacat aaaggatatt accatttcgt tttttaccaa
    10561 tttcttttgc aagtttgatt ctttgggatt caccaccact taaagttggt gtaggttggc
    10621 ctaattttat ataccccata cctatttgtt ctaatacatt taagtgtgaa acaatatttg
    10681 gaatatcctt gaagaaatgt acagattcag aaacactcat atttaaaata tcatgtatat
    10741 ttttattttt atatttaata gataaagatt catcattaaa tctttttccc tgacattcac
    10801 tgcatatatg gtctatactt agattatttc ccaaccagac cttttcatat ccactaccac
    10861 tacatttagg acaagcccct ttggagttaa atgagaaatg accagctgta aatcctcgtt
    10921 ctaacgattc tggctgacct gcaaatagtg tacgtatctt atcccatata ccaatataag
    10981 aagcaggagt tgagttcata tttttgccaa taggagattg agatatctct gaatatccag
    11041 aaatatgttt agtaccaatc agtctatctg caatagtttc aacaactgca taatcgtctt
    11101 cctcacctat ttcactctct gaagaattta tttgattatt gatagtatgg ttattaaatt
    11161 ggcttctaag aagaggaagt agggtgtctg atataagtga actcttgcca ctacctgatt
    11221 ttccagatat tccaaccata gcacccaaag gtaatgatac agttacattt ttaagattat
    11281 ttgtatttgc attttgaata gttatacaag gtattttatc agtatgtgta atgtttttat
    11341 tgcttgttct attaggcatt ggatattttc cagatagata ctgtccagtg agtgatttgt
    11401 cggattgaag tagaccttct aaatctcctt gatatactaa ttgaccacct tcaacaccag
    11461 cttttggacc aatatcaata atatgttctg ccattttaat tgtgttttta tcatgctcaa
    11521 ctactatgac cgtattgcct aaatctttaa gtttctttat tgattttaag atgtctattt
    11581 tttctgattc atgcaatcca gctgttggtt catcaagcac ataaattaat gagtccatct
    11641 ctgaatcgag atgtgaattt aaaaatagtc tttgcatttc cccaccactc aaagttgaca
    11701 tttctcggta taaggataga tgacctaatc gtgattctat taaatgttga gttttggata
    11761 aaatatcatt tacaagattt gtaccaaatg tgtttaaatt accttgattt attaagagtt
    11821 taaggaaatc atcaatttct aaaatcgtca tttttccaag ttctccaata tgttttccgt
    11881 ttaaaagtac tctacgtgct tcttcaccaa ttttaaaacc attgcaatct gaacaggcag
    11941 tcatagtata aactccattt aaatcttcgc cccttttgta acgattgtga aatacctttg
    12001 ataaagagga acttttttta ccattttcat aatgaccata gagtacttca tttcttactt
    12061 cttcaggtag ttgtaagaat tgtatgtcta aataatcatt gaatttttta gaaaatactc
    12121 tcaaatatcc aggtgtaatc tttgctctat ctaagacttg ttttagggta tcatgcttat
    12181 caaggactaa tttttcaata ttaatctcaa aatatgcacc atgtccagag cacttcatac
    12241 acattccatc tgaagagttg tatgagaaat aacttggtct taatcgttct tcctcatatc
    12301 cacaattatt gcaaattaga ttatagctta caggagtatc acacatagta cacattattt
    12361 gaccttccac tgaatacaat agtgtaagca tgttgagaat attggttctt gtgcctactg
    12421 ttgaacgagg attgctctgg cgaataatat tttgttgaac agctatagtg ggagctaaac
    12481 cttgaatatt atcaaactta tcttcactat caatacctgc aagtatacct aaagattgca
    12541 gatattgttt acgcccttcc tcaaatacaa tgtcaaacat aaggctagac ttacctgagc
    12601 cactgacacc tgttgcaaca acaagcttat ctttggggat agaaacatct atatttttaa
    12661 gattgtgtat ctttgctcca ttaattttta tttctttcat tggttcacct cattttatca
    12721 attttatcaa accgtaaaat agggatatat taatccttaa tacaagaata gattaggtga
    12781 ggttattaaa aaggttctat aggtaaatat atatcgacca agtgtctatt ttgagggtga
    12841 ttgtgtgggt tgttaagata tacttcaaaa gggaaggagg cacgtagctt ataattacca
    12901 tttgatagcc attcattata cataaaatcc cacgcatcac tatattcaga ttttgaaata
    12961 tcaaaatgtc ccacaagata ttttccagaa ggtattatca tattgccaag attgtcattc
    13021 tctttgacaa caatgttatc tggaatggtc atgcaaatac tagtccttaa ttgatgctcc
    13081 tgtgtaaatt gtggattatc atgataaatg gctaatattt gtgtgttttc aagtccaagt
    13141 agattctgct cacttgcata tgtgaataac cgttctaaaa gttttgggaa agtactggtt
    13201 agattaccat atgaaccagt atacctagtg taaatagtat tcatattatc aagttctaga
    13261 atttcaattt cacctttaac ctctctagta ttcttacatt taggttttga tatgcttcta
    13321 ttgtactgag aaattttata tggttctttg caattcttgc tataattgtt tctatatttt
    13381 atagggctta ttttatagta atttttaaat gctcttgaga aaactgctga atcagtaaag
    13441 ccaaagtagt gtgcaatatc agtaacagtc atgtcagttc tatgaataag aaaagcagtt
    13501 gcattttcta gttttagcct atttacatat tgtgccaaag tttctttttc tattgctttg
    13561 aagattctat gaaaatgaaa ttttgaaaaa cctgctatat tagaaagttc atcaagagaa
    13621 agaggttcat gtaaatgttc ttctatatag tcttgcactt tgcaaatacg atttagatat
    13681 tcttttttac ttttagcatt tgtcatatat agccctcctt attgtgtaaa atgtgtatca
    13741 attaatttat agcaatatag taataaaatt ttatgttttt gtaatttatg ataaataaga
    13801 tagcgtttaa ttttttataa ttgataaaag atatttataa ttaataaaag atatttataa
    13861 ttaataaaag atataagatg ccttatattt gtctttgcat ataagtatag tcttcgtaat
    13921 aattagtata ccatagtgat ttatttttaa ttgtgtattt atataaatac acaacttatg
    13981 atatggacga attattatgc aaataagctg taattaaata aattgcaata taaaccagtt
    14041 gaaacataag ttagggaaat attatcaaag ttattactta gcataagagt atagatggaa
    14101 taaaataaga ttaaaaaatt acatactaaa tttaataaaa tttttatatt aaaaataata
    14161 ttaag
//
LOCUS       DAMJYR010000095        14094 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.098, whole genome shotgun sequence.
ACCESSION   DAMJYR010000095 DAMJYR010000000
VERSION     DAMJYR010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14094)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14094)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14094
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.098"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            487..1380
                     /locus_tag="PC471_002737"
     CDS             487..1380
                     /locus_tag="PC471_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfide/dihydroorotate dehydrogenase-like
                     FAD/NAD-binding protein"
                     /protein_id="HDF2789851.1"
                     /translation="MSNKIVSKRQLTDSIYLMEIEAPRVAKSSQPGQFIIIKNDEKGE
                     RIPLTIADYDREKGTVTIVFQTVGASTKKLAMFEENDFVMDFVGPLGQASEFIHEDIE
                     ELRNKKILFVAGGVGSAPVYPQVKWFKEHGLDVDVIIGARTKELIILEDDMKKVAKNV
                     YVSTDDGTYGFNGRVTDLLKDLVDNQGKKYDQAIVIGPMIMMKFMCQLTKELNIPTIV
                     SLNTIMIDGTGMCGGCRVSVGNETKFACVDGPEFDGHLVDFDQAMRRQSMYKTQEGRA
                     MLKLEEGDSHHHSNCGCGGNK"
     gene            1380..2774
                     /gene="gltA"
                     /locus_tag="PC471_002738"
     CDS             1380..2774
                     /gene="gltA"
                     /locus_tag="PC471_002738"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent glutamate synthase"
                     /protein_id="HDF2789852.1"
                     /translation="MDAKKVKVPVREQEPAVRATNFDEVCLGYNKEEAMAEANRCLAC
                     KKPKCVGGCPVGIDIPGFITKIKEDDIEGAAKVIAKSSSLPAVCGRVCPQESQCEGVC
                     ILGIKSDAVSIGKLERFVADWSKENDINLSDTEPKKNQKVAVIGSGPAGLACAGDLAK
                     KGYDVTIFEAMHEPGGVLTYGIPEFRLPKQAVVQPEIDNIRKLGVKIETNVIVGKTIT
                     VDELIEDEGFEAIFIGSGAGLPMFMNIPGENANGVFSANEFLTRVNLMKAYRDDYDTP
                     ISSGKKVAVVGGGNVAMDAARTALRLGSESYIVYRRSEKELPARAEEVHHAKEEGIIF
                     NTLTNPKEILVDENGYVKGMVCIRMELGEPDDSGRRRPIEIEGSEFVLDVDTVIMSLG
                     TSPNPLISSTTKSLDINKKRCLITDENGQTSKEGVFAGGDAVTGAATVISAMGAGKTA
                     AASIDEYLKAKVNA"
     gene            3140..5359
                     /locus_tag="PC471_002739"
     CDS             3140..5359
                     /locus_tag="PC471_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HDF2789853.1"
                     /translation="MKKREKVFDGLVFKHILILLILLVLIDLTSTIIYASNSMEITSR
                     NMIEASKRELENYLEVNISLLKALSQDDRFSNGETSLIEKGKLLSPYQKEYNLFMIGI
                     TDTKGNASSTYRENVNSIKDKPSFEKAIKTKQVVVSDIEVSNVTGDKVFIIYVPIVKN
                     NEMIGTIFASFYFQDVNNLISRSNFDDSIKFLMIDKDYTIISHPNKKYVNDKSKMLDL
                     EGNIIGTTKSEILKNINKKCQGDFLSWDNWRLYNVKYTNIKWTNWTLVSKCNIFKNFK
                     NLIVNFMIKLYFYIVIFMILWKLSNAKLIEQLKKLAYYDSLSGIKNKEKFRKDSMYIL
                     KNYYQDNFYLVQLDVNKFKYINEMFGYAEGNKILIHISQVLNNNTNKYEICARMDNDH
                     FILLIACNTEDELLNRLSKINKEICNLNTTNSSKYKIVMSSGIYKITKKDDIKKIDLL
                     IDRANIAAKSKKEKYEHSYSFFNEDTRNRLYKEKRLEDNMNKALEKGEFIVYYQPKYS
                     LDDVNEIEGAEALIRWNSPEFGFISPIDFVPLFEKNGFIVNIDMFVFEEVCKTLNKWI
                     NKGYTPVPISVNMSRVHLYRDNFIENITDLISKYNISPEFIELELTESVVFDNLNILI
                     DIMKKIKEIGFLISMDDFGSGYSSLNLLKDLSFDILKLDRGFLIETTDTKRGKIIISK
                     IVEMAKAIDIKVICEGVETYEQVEFLKEIGCDKVQGYLFAKPMVLDEFEKHLNFKFD"
     gene            5506..6252
                     /locus_tag="PC471_002740"
     CDS             5506..6252
                     /locus_tag="PC471_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014466367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2789854.1"
                     /translation="MNILSIRNISKTYCGNIPFKALDKVSLNIEKGEFVSVMGPSGSG
                     KSTLLNIISTVDRQSEGEVVLDGYDVSKLKGEKLAEFRRKQLGFVFQDFNLIDTLTVG
                     ENIMLPLTLEGASIKDMNIQTKSISKFLGIDKILDRKTYEISGGQAQRCAIARAIINK
                     PAILLADEPTGNLDSKSTDDVLKLFTRINKEQKVTTLMVTHEAYSASYSDRVIFIKDG
                     CIYTEIKKSESSNSFYSDILAVLSQIGGVR"
     gene            6252..8132
                     /locus_tag="PC471_002741"
     CDS             6252..8132
                     /locus_tag="PC471_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2789855.1"
                     /translation="MDFRKFAYNNIVKNFRAYLAYFLSSVFSIMIFFVFAVSMFHPII
                     TKSGMQSGSTAFMALMTSELIIFGFSLLFILYSLGNFLKYRLKDFGVLMIIGMSNRQL
                     KKLILIENLIIGFLAVMLGILLGLSISKLFLLYLSKLFYMNLTEFYFPVKAMLLTIVS
                     FMILFLITGPMSLKLLSKKSILELLVGTKKPKKEIKSSKLFGIIGVICLILGYAMILF
                     GNKLKINGGMYTTALLITLGIFLFFSQFTILILKYLKNKEKFYKNKVNMLLISNLIYK
                     LKDNTRLLFLITILLSATLVAFTTTSTLVSSQGEASKNNFPMVYSYFSEHNNEKEYEE
                     LQQIREAIKGYDYKELSFPVLKYNREVLLSVKNYNKLSKELGLDKISSKLEKYEGVII
                     PSSNSQEHINSLKQLKNYSIKNLNIEVKKSKNKIIFPTGLFSKVVVISDDLYNEVKGE
                     FSKINFHGFDYKNWQSSAQITENLKEKHFNLDREYNRSYFLTLPDMYLVELQQSKILQ
                     FMGVFIGVILFIAVFSFLYFRLYTDEIEDNVKYKKLSKIGLSFKNMNKIVSIEIGTLF
                     FIPYFIAIINSIISLLFLNQVLNNSFKLENLGILLLISSIYSLYFYLLKKIYTRKVWF
                     FD"
     gene            8251..9642
                     /locus_tag="PC471_002742"
     CDS             8251..9642
                     /locus_tag="PC471_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HDF2789856.1"
                     /translation="MENQQLLGTERISKLLLKYSIPAIIGMLVNSLYNVVDRIFIGNI
                     PGVGPLAITGLGVTMPIMTIILAFGMLIGIGTTTTISIKLGQGKVEEARKLIGNAMTL
                     SVITGIIIMILGILFANKILTLFGASENTLIYAKSYINIILLGTVVNLLSFSLNHSIR
                     ADGSPKISAGIMIVGCLTNIVLDWILIFGFNLGIQGAAIATVTSQALTAILTIGYYIS
                     GKSNLRFSKSNLKLDKKLIKAVFAIGMSPFAMQLAASLVQVISNIALKTHGGDLAIGA
                     MATISSIAMVFLMPIFGINQGAQPIIGFNYGAEKYDRVKKAYLGSLVVATIILCMGMV
                     VVMLFPEAIIGIFNKDPELMNISVNGLRIYLLMLPIVGLSVTGTNFIQSIGKAKMAML
                     LSLLRQVILLIPAVLILPTFLGLQGVWTAQPVSDFIATVITGIVVFRELKRYTPKTEK
                     LNENERLNEITTE"
     gene            9749..10789
                     /locus_tag="PC471_002743"
     CDS             9749..10789
                     /locus_tag="PC471_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HDF2789857.1"
                     /translation="MEFTKKSKLKNMYVNMNVARMLSKINEYKGRQLLYKKQPKEILE
                     NLEKKSLVDCSESTYIGNQRDSSNFNLEKLISNEVTPRSREELSIVEYRDVVKTINSA
                     YESIPISSQTILELHGYLYKFSSTRGGSYKSDNDFIEHNLKPSEFISIDSSVNKVEKA
                     VEEICEAYNTLIEEDEIDILILISAFVLDFILIHPFKEGNIKMARVLILLLLNKNGYE
                     VGRYISLGKIFDDSSYEYYSNLNYLKASIGSEKADMNAWIEYFLETILTAYEKLDDSL
                     NISDKKRQTKTSRIEKIINSTLGYFTKEDIRDLCPDIPEPTINRVFNNLRKQDKIEVV
                     ARGRSAKWKKKY"
     gene            11278..11931
                     /locus_tag="PC471_002744"
     CDS             11278..11931
                     /locus_tag="PC471_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HDF2789858.1"
                     /translation="MKKKLLYINVNSKPEDLSSSKTVARKFINKFMEKNKDFEVEEID
                     LYKEHIPRLEYQYFEKRNSIVSEENAKNLDDKDRKELTKIRNLCDQFVSASVYVIAAP
                     MWSLSFPAPLKEYIDCIIQDGKTISFEGNDKPQGILNDIDRSMVYIQSSGGHIPWVLK
                     PVMNKGLNYVESIMKFIGIKKFDELLVDGTGTSEEERQAAIEKAAEKIDGIIDGMKF"
     gene            12007..12216
                     /locus_tag="PC471_002745"
     CDS             12007..12216
                     /locus_tag="PC471_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2789859.1"
                     /translation="MREEKSNEKYDCYWCNQENNFCVEIKDNIVMIDDGTGTLKQAVF
                     IGYKQIQINLNCSHCQNLNRIKLNL"
     gene            complement(12356..12922)
                     /locus_tag="PC471_002746"
     CDS             complement(12356..12922)
                     /locus_tag="PC471_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HDF2789860.1"
                     /translation="MMNNELNTIILETLNNADITSNDIPSIDLYMDQIISLIDNKLSA
                     NKRFESDKILTKTMINNYSKEGLIKPVKGKKYTKEQILQMIIIYSMKNTLTIQEIKRI
                     LHGVYEKDNFSEKDLVSCYEKFMLIKENQRKNIPDFIESNFENISINPENKDDLLIAL
                     LSLTSMADQLKNISEKLVDRYFPDITKK"
     gene            13083..13736
                     /locus_tag="PC471_002747"
     CDS             13083..13736
                     /locus_tag="PC471_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HDF2789861.1"
                     /translation="MYQFFLKGRDPISSLTHFIGACLSLLATIILVFQSVTLQETSLL
                     MIVSVSVFGFSLIALYSASSYYHFLKGTPEQELFFRKVDHAMIYVLIAGSYTPICLNF
                     MEKKEGIIFVTAIWIVAFIGIIIKIFWMDAPRWLSTSIYLLMGWAIVFDINAFNSIPK
                     DCLRLLIMEGVSYSIGAIIYIIKKPNISPEFGFHEIFHIFIMIGSLFHFLAVLLYVL"
     gene            complement(13894..14070)
                     /locus_tag="PC471_002748"
     CDS             complement(13894..14070)
                     /locus_tag="PC471_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2789862.1"
                     /translation="MYKYDDYVNLTNDLDLPVEIRNKCVRIVSVSENLEDIIIVHKFK
                     LYVVNEKYIKGIME"
BASE COUNT         5520 a         1389 c         2384 g         4801 t
ORIGIN      
        1 aattaatgaa tattgaaaat tttagatata taataaaata agagtatatt ttatacaata
       61 tgaaagctgg tattgcaata gattaaagcc ttatacacgt ttagttttta aagaaattag
      121 tgttattatg aaaacctaag ttaatttatt aaataaataa ttgaataaaa aaattttatt
      181 atgtatttat aaaatttttg ttacaaattt ggcataatat agtatacttt aagtgtgtat
      241 taggttaaat ctataacaaa ttagacgtat gatatagact tagtgtttat tggcatttac
      301 aagaaatttg ttaatatcaa ctatttgtag tttgggcagt tataattatt atagggggga
      361 tgtccaatac aaaacaatca accaagaact aatattatat tataaagagt tatagttttg
      421 tgtgtttatg tttttaatta aatttaaagt gggaaaaata tatttgttag aatggagaat
      481 agcgagatga gtaataaaat agttagtaag agacaattaa cagatagtat ttatttaatg
      541 gaaatagagg cacctagagt tgcaaaatct tctcaaccag gtcaatttat aataattaaa
      601 aatgatgaaa aaggagagag gattcctctt acaatagctg actatgatag agaaaaagga
      661 acagtaacta ttgtattcca aacagttggt gcttcaacta aaaagttagc aatgtttgaa
      721 gaaaacgatt ttgtaatgga ttttgtaggg ccattaggac aagcaagtga atttatacat
      781 gaagatatag aagaattaag aaataaaaag atattatttg tagctggtgg agttggttct
      841 gctccagttt atccacaagt taaatggttt aaagagcatg gattagatgt agatgtaata
      901 attggtgcaa gaactaaaga attaattata ttagaagatg atatgaaaaa agtagctaaa
      961 aatgtatatg tatcaacaga tgatggtaca tatggattta atggaagagt tacagaccta
     1021 ttaaaagatt tagttgataa tcaaggtaaa aaatacgacc aagcaatagt aattggacca
     1081 atgataatga tgaaatttat gtgccaatta actaaagaac taaatatacc tactatagta
     1141 agtttaaata caataatgat agatggaaca ggtatgtgtg gtggttgtag agttagtgtt
     1201 ggaaatgaaa ctaagtttgc atgtgtagat ggtcctgaat ttgatggtca tttagttgat
     1261 tttgatcaag caatgagaag acagtctatg tacaagactc aagagggaag agctatgcta
     1321 aaacttgaag aaggcgatag tcatcatcat agtaattgtg gatgtggagg taacaaataa
     1381 tggatgctaa aaaagtaaaa gtaccagtta gagaacaaga acctgctgtg agagcaacaa
     1441 attttgatga agtatgctta gggtataata aagaagaagc tatggcagaa gctaatagat
     1501 gtttagcttg taaaaaacca aaatgtgtgg gtggatgtcc agtaggaatc gacataccag
     1561 ggtttataac aaaaattaaa gaagatgata tagaaggtgc tgctaaagta atagctaaga
     1621 gcagttcatt accagctgtt tgtggtagag tatgcccaca agaaagtcaa tgtgaaggtg
     1681 tatgtatact tggaataaaa tcagatgctg tatcaatagg taaattagaa agatttgtag
     1741 cagattggtc aaaagaaaat gacataaatt tatctgatac agagcctaag aaaaatcaaa
     1801 aagtagcagt aataggaagt ggtccagcgg gtctagcttg tgctggtgat ttagctaaaa
     1861 aaggctatga tgtaacaatc tttgaagcaa tgcatgagcc aggtggagtt ttaacttacg
     1921 gaatacctga gtttagactt ccaaaacaag ctgttgttca acctgaaata gacaatataa
     1981 gaaaattagg tgtaaaaata gaaactaatg taattgttgg taaaacaatt acagtagatg
     2041 aacttataga agatgaagga tttgaagcta tattcatagg ttcaggagct ggtcttccaa
     2101 tgtttatgaa tataccaggg gaaaatgcaa atggagtatt ttctgcaaat gagtttttaa
     2161 ctagagtaaa cttaatgaag gcttatagag atgattatga tacacctatt agttctggta
     2221 aaaaagttgc tgtagtaggt ggtggaaatg ttgctatgga cgctgctaga actgcattaa
     2281 gattaggttc agaatcatat atagtttata gaagaagtga aaaagagctt ccagcaagag
     2341 ctgaagaagt tcatcatgca aaagaagaag gcattatatt taatacatta acaaatccta
     2401 aggaaatttt agtagatgaa aatggatatg ttaaaggtat ggtttgtata agaatggaat
     2461 taggagagcc agatgattct ggaagaagaa gacctataga aatagaaggt tctgaatttg
     2521 tacttgatgt agacacagtt ataatgtcac ttggaacaag cccaaatcca ctaatatctt
     2581 ctacaacaaa gagtcttgat ataaataaga agagatgctt aataacagat gaaaatggtc
     2641 agacttctaa agagggtgta tttgcaggtg gagatgctgt tactggagca gcaacagtaa
     2701 tatctgcaat gggtgctggt aaaacagcag cagcatcaat agatgaatat ttaaaagcaa
     2761 aagttaatgc ttaatttgta aattaaagag agggtgtctc aaaatgaact ttttagttca
     2821 tgaggcactc tttttgtaca aaatcaaata tgttttaatc atttcaacaa tgaaaatttc
     2881 attcctgtag aaagtctaac ttttaggtct tagtacaatc tctattttga tgtagccact
     2941 tttttagatg gtttaataat ttatcagtta ttattgtaat atttgatact aagtaacata
     3001 actttgtcta actatgataa aatagttata caataaaggt cttgtaagga gtgaataatg
     3061 tgtggctaat aaaatcagat ttgtcaaatc taacattaaa taatagaaaa ataaattgtg
     3121 taattaatgg tattttaata tgaaaaaaag agaaaaagta tttgatgggc tagtttttaa
     3181 gcatatatta atcttattaa tactattagt tttgattgat ttaacaagta ccatcatata
     3241 tgcaagtaac tctatggaaa ttacttctag aaatatgatt gaggcatcaa aaagagaact
     3301 tgaaaactat ttagaagtaa acatatcttt attaaaagct cttagtcaag atgatagatt
     3361 ttctaatggt gaaacatcac taattgaaaa gggaaaatta ttaagtccat atcaaaagga
     3421 atataatctt tttatgattg gtataacaga tacaaaagga aatgcttcaa gtacatatag
     3481 ggaaaatgtt aactcaataa aagataagcc tagttttgag aaagcaatta aaacaaagca
     3541 agtagtagtt tcagatattg aagtcagtaa tgtaacaggt gacaaagtat ttatcatata
     3601 tgtaccaatt gtaaaaaata atgagatgat tgggaccatt tttgcttctt tttattttca
     3661 agatgtaaat aatttaataa gtagaagcaa ttttgatgat tctattaaat ttctaatgat
     3721 agataaagat tatactatta tttctcatcc aaacaaaaaa tatgtaaatg ataaaagtaa
     3781 gatgcttgat ttagaaggga atattatagg aaccactaaa agtgaaatct taaaaaatat
     3841 aaataaaaaa tgtcaaggtg attttttatc ttgggataat tggagattat ataatgtaaa
     3901 atatacgaac ataaagtgga ctaattggac tttagtatca aaatgtaata tatttaagaa
     3961 ctttaaaaat ctaatagtta attttatgat aaagttgtat ttttatatag taatatttat
     4021 gatattgtgg aaattaagta atgctaaatt aatagaacaa cttaaaaaat tggcttatta
     4081 tgattcttta agtggaataa aaaataaaga aaagtttaga aaagattcta tgtatatttt
     4141 aaagaattat tatcaagata atttttactt agtacaattg gatgtcaata aatttaaata
     4201 tataaatgag atgtttggat atgctgaagg gaataagata ttaattcata tatcacaggt
     4261 tttaaataat aatacaaata aatatgaaat ttgtgctagg atggataatg accattttat
     4321 attgttgatt gcttgtaaca ctgaagatga acttttaaat agattatcta aaataaataa
     4381 agaaatttgt aacttaaata ctactaactc atctaagtat aaaattgtta tgtcaagtgg
     4441 aatatataag attactaaaa aggatgatat taaaaagatt gacttgttaa ttgatagagc
     4501 aaatattgca gcaaaaagta aaaaggaaaa atatgaacat tcatattcat tttttaatga
     4561 agatacaaga aatcgattgt acaaggaaaa acgattggaa gataatatga ataaagcttt
     4621 agaaaaaggt gaatttatag tatattatca acctaagtat agtttagatg atgtaaatga
     4681 aattgaagga gcagaagcct tgataagatg gaatagtcca gaatttgggt ttatttcacc
     4741 tatagatttt gtacctttat ttgaaaagaa tggttttatt gtaaatattg atatgttcgt
     4801 gtttgaagaa gtatgcaaaa cattaaacaa gtggataaat aaagggtata ctcctgtacc
     4861 aatctctgta aatatgtcta gagttcattt atatagggat aattttatag aaaatataac
     4921 tgatttgata agtaaataca atatatctcc tgagtttatt gaacttgaac taacagaaag
     4981 tgttgtgttt gataatttaa atatattgat tgatataatg aaaaagatta aagagatagg
     5041 attccttata tcaatggatg attttggttc gggatattca tctttaaatc tgttaaaaga
     5101 cctttctttt gatattttaa aattagatag aggattttta atcgaaacaa cagatacaaa
     5161 acgtggaaaa attattattt ctaagattgt ggaaatggca aaagcgattg atattaaagt
     5221 aatctgtgag ggcgtagaaa cttatgaaca agtagagttc ttgaaagaga taggttgtga
     5281 taaggttcaa ggatacttat ttgctaaacc tatggtatta gatgaatttg aaaaacactt
     5341 aaactttaaa tttgattaat cattaataat tattagacta taatataccc taacaactta
     5401 cttacaaata tgtaagttaa ttgttagggt atttttatgg ttaaagtagt atattcacga
     5461 tattatggta ttgtagtaaa aataaaaaat tatggagaat ataaaatgaa catattgagt
     5521 attagaaata tatcaaaaac atattgcgga aatattccat ttaaagcatt agataaggtt
     5581 agtctaaata tagaaaaggg agaatttgtg tctgttatgg gaccatctgg aagtggtaaa
     5641 tctactcttt taaatataat atctacagtt gatagacaga gtgaaggtga ggttgtatta
     5701 gatggatatg atgtaagtaa attgaaagga gaaaaacttg ctgaatttag aaggaaacaa
     5761 cttggttttg tatttcagga ttttaatttg attgacactt taactgttgg cgaaaatatt
     5821 atgttgccac ttacactaga gggggcaagt atcaaagata tgaatataca aactaaaagt
     5881 atatctaaat ttttaggaat agataaaatt ttagatagaa aaacctatga aatttcagga
     5941 ggtcaagctc aaaggtgtgc tattgctaga gctattataa ataaacctgc catacttctt
     6001 gcagatgagc caactggaaa tcttgattca aaatctactg atgatgtttt gaaattattt
     6061 actaggataa acaaagaaca aaaggtaaca acacttatgg taactcatga agcatatagt
     6121 gcaagttatt cagatagagt aatttttata aaagatggtt gtatatatac agaaataaaa
     6181 aaatctgaaa gcagtaattc tttctattct gatatactag ctgttttgtc tcaaatagga
     6241 ggtgtaagat agtggatttt agaaagtttg cttataataa tatagttaaa aatttcaggg
     6301 catatttagc atatttttta agttctgttt tttctataat gatatttttt gtttttgcag
     6361 tgtctatgtt ccatccaatt ataacaaaat ctggaatgca gagtggttct actgcattta
     6421 tggctcttat gacttcagaa ttaataatat tcggttttag tctacttttt atattgtact
     6481 ctttaggaaa tttcttgaaa tatagattga aagattttgg agtattgatg ataattggaa
     6541 tgagtaacag acaactgaaa aaattgatat taattgaaaa tttgataata ggatttttag
     6601 cagtgatgtt aggaatatta cttggtttat caatttcaaa attgttttta ttgtatttaa
     6661 gcaaactgtt ttatatgaat ttaactgaat tttattttcc agtaaaagca atgctattga
     6721 ctattgtatc atttatgatt ttatttttga taacaggacc aatgagctta aaattattga
     6781 gtaaaaagag cattttagag cttttagtag gtacaaaaaa gcctaaaaaa gagataaaat
     6841 cttcaaagct atttggaata attggtgtaa tatgtcttat attaggatat gctatgatac
     6901 tatttggcaa taagttaaaa ataaatggag gtatgtatac aacagcacta ttaattactt
     6961 tggggatatt tttatttttt tctcaattta ctattttgat attaaagtat ttaaaaaata
     7021 aagaaaaatt ttataagaac aaagtaaata tgttacttat aagtaatttg atatataaat
     7081 taaaggataa tacacgattg ttatttttaa taacaatact tttatcggct actttagtag
     7141 catttacaac aacttcaaca cttgtaagtt ctcaaggaga agcttctaaa aataattttc
     7201 caatggttta ctcatatttt tcagagcata ataatgaaaa agaatatgaa gaactacaac
     7261 aaattagaga agcaatcaaa ggatatgatt ataaggagtt aagttttcct gtacttaaat
     7321 ataataggga agtcttacta agtgtaaaga attataacaa attatcaaag gaattgggat
     7381 tagataaaat tagttcaaag ttagaaaaat atgaaggggt tataattcct agtagcaatt
     7441 cacaagaaca tataaattct ttaaaacaat taaaaaatta tagtataaaa aatttaaata
     7501 tagaagtaaa aaaatcaaaa aataaaataa tttttcctac aggactgttt agtaaagttg
     7561 tagttataag tgatgactta tacaatgaag taaaaggtga attttcaaaa attaattttc
     7621 atggatttga ttataaaaac tggcaaagca gtgcacagat aactgaaaat ttaaaagaaa
     7681 aacattttaa tttggataga gaatataaca ggtcatattt tttaacatta ccagatatgt
     7741 atttagtaga gttacaacaa agtaaaattc ttcaatttat gggtgttttt ataggagtga
     7801 tattatttat agcagttttt agttttttat actttagatt atatacagac gagatagaag
     7861 ataatgtaaa gtacaaaaaa ctatcaaaaa taggtctatc ttttaaaaat atgaataaga
     7921 ttgttagtat agagataggt acattgtttt ttataccgta ttttatagca attataaata
     7981 gcataatttc tttactgttt ttaaatcaag tattaaataa tagttttaag ttagaaaatt
     8041 tgggtatatt actactgata tcatctatat attcacttta tttttactta ttaaaaaaaa
     8101 tttatactag aaaagtttgg ttttttgatt aaagtggtat ataatagata tataaattaa
     8161 tatttaaaaa gtagttaata ttaggggagg gcatttgcac ctcccttgta atatgtggaa
     8221 aattgaatat tagaagaagg aggagacttg atggaaaatc aacaattatt aggaacagaa
     8281 cgaattagta aattactact aaaatattct ataccagcta taataggtat gttggtaaat
     8341 agtttatata atgtagtaga tagaattttt attggaaata taccaggggt tggaccactt
     8401 gcaataacag gtcttggtgt aacaatgcca ataatgacaa taatacttgc atttgggatg
     8461 ttgataggta taggaactac tactaccata tcaataaaac ttgggcaagg taaagtagaa
     8521 gaggcaagaa aacttatagg aaatgcaatg acgttatctg taataacagg aattatcata
     8581 atgatacttg gaatattatt tgcaaataaa atacttacat tgtttggagc aagtgaaaat
     8641 actttaatat atgcaaaatc atatatcaat attatattat taggtacagt agtaaatctt
     8701 ctttcctttt ctttaaacca ctcaataaga gctgatggta gtcctaaaat ttcagcagga
     8761 ataatgatag taggatgttt aactaatata gttttagact ggatattaat atttggattc
     8821 aatttgggaa tacaaggagc tgcaattgct acagtaactt ctcaggctct tacagctatt
     8881 ttaactatag ggtattatat aagtggaaaa tcaaacttac gattcagtaa atctaatcta
     8941 aaattagata aaaagctaat aaaagcagtg tttgcaatag gtatgtcgcc atttgctatg
     9001 caacttgcag caagtttagt tcaagtaata tcaaatattg cacttaaaac tcatggtgga
     9061 gatttagcta taggggctat ggctactatt tcttctattg caatggtatt tttgatgcct
     9121 atctttggaa taaatcaagg agcgcaacct attataggtt tcaattatgg agctgaaaaa
     9181 tatgataggg ttaaaaaagc atatttaggt tcattagtag tagctactat aattttatgt
     9241 atgggtatgg tagtagttat gcttttccca gaagctatta taggtatatt taataaagac
     9301 cctgaactta tgaatatatc tgttaatgga ttaagaatat acttacttat gttgccaatt
     9361 gtaggcttat cagttacagg aacaaacttt atacaatcta taggaaaagc aaaaatggct
     9421 atgttattga gccttttaag acaagtaatt ttattgatac cagcagtatt aattcttcct
     9481 acattcttag gtttacaggg tgtatggaca gctcaaccag tgtctgattt tatagctaca
     9541 gtaattacag gtatagttgt atttagagaa ttaaaaagat atactcctaa aactgaaaaa
     9601 ttaaatgaaa atgaaagact aaacgagata actactgagt aaaaatataa attcagataa
     9661 ttgagcaaga aattgcaaaa aatacgatta tatgataaaa taaaaccgct gtcaaggata
     9721 agagaaattc taattatgga gtgattttat ggaatttaca aagaaaagca aattaaaaaa
     9781 tatgtatgta aacatgaatg tagctagaat gttgtctaag ataaatgagt ataagggtag
     9841 acaactgctt tacaaaaaac aaccaaaaga aattttagaa aacttagaaa aaaaatcttt
     9901 agtagattgt tctgaatcta catacattgg aaatcaaaga gatagtagta attttaatct
     9961 agaaaagttg ataagtaatg aagttacacc tagaagtaga gaggaattga gtattgttga
    10021 atacagagat gtggtaaaaa ctattaacag tgcttatgag agtattccca tcagttcaca
    10081 aacgattctt gagcttcatg gatacttata taaattttca tctactagag gaggaagtta
    10141 taagtcagat aatgatttta ttgaacacaa tttaaaacct agcgaattta taagtattga
    10201 ttcaagtgtc aataaagttg aaaaagctgt agaagaaata tgtgaagctt ataatacatt
    10261 gatagaggaa gatgaaattg atatattaat attaatttct gcatttgtgt tagatttcat
    10321 attgatacat ccattcaaag aaggtaatat aaaaatggcg agagtactta tcctattgtt
    10381 actaaataaa aatgggtatg aagttggaag atatataagt ttaggaaaga tatttgatga
    10441 tagttcatat gaatattata gtaatttaaa ttatttgaaa gcctcaatag gtagtgaaaa
    10501 agcagatatg aatgcatgga ttgagtattt tttagagacg atattaactg catatgagaa
    10561 gctggatgat agtttaaata tatctgataa gaaaagacaa acaaaaacta gtagaataga
    10621 gaaaataata aattctaccc ttggatattt tacaaaagaa gatataagag atttatgtcc
    10681 tgacattcca gaacctacta taaatagagt ttttaataat ctaagaaaac aagataagat
    10741 agaagttgtt gcaagaggaa gaagtgcaaa atggaagaaa aaatattaaa aaatgtttag
    10801 ataactaaaa tggtatagtg ttttacacta taccattttt aatgtcatta gcatgagcaa
    10861 taatttaaca acttaagcaa tatcaatttt atttattgat tatttttgaa ctgaaaaatg
    10921 gcgttatatg gtatacctca gtggtaatta attttggatg agaagatata atattacaga
    10981 atatattagg agtatatgta gaaaaaatat aataaaagtg tgaggcttag taattaacaa
    11041 attatgtggc ttcacacttt tttataaaat aaagatactt agcagaagaa tattttagtt
    11101 gatatgttta taagatggta cttgtttggg taaaagtata aatagtaaaa aagtagaagt
    11161 aatgtgtata aaaacgtaag ttacattctg taataatagt tattttataa ttcaacaaaa
    11221 gaatagaatg ggtatatttg tgaaataata atataaaata atcttaggag tgagcttatg
    11281 aagaaaaaat tattatatat aaatgtaaat tcaaaacctg aagacttatc ttcaagtaaa
    11341 actgtagcaa gaaaatttat aaataaattt atggaaaaaa ataaagattt tgaggttgaa
    11401 gagatagacc tttataaaga acatatacca agacttgagt atcagtactt tgaaaaaaga
    11461 aatagcatag ttagtgaaga aaatgctaaa aatttagacg ataaggatag aaaagaactt
    11521 acaaagatta gaaatctttg tgaccaattt gtgagtgcaa gtgtatatgt aatagcagca
    11581 cctatgtgga gtttatcttt tccagcacca cttaaagagt atatagattg tataattcaa
    11641 gatggaaaaa ctatatcttt tgaaggcaat gataaaccac aaggaatttt aaatgatatt
    11701 gatagaagta tggtatatat acaatcttct ggtggacaca taccatgggt gttgaagcca
    11761 gttatgaata aagggttaaa ttatgtggaa agtattatga aatttatagg aattaaaaaa
    11821 tttgatgaat tgttagtaga tggaactgga actagtgaag aagaaagaca agctgctata
    11881 gaaaaagcag ctgaaaaaat agatggtata atagatggga tgaaatttta attataatat
    11941 tttattaaaa atataaagga aaaacctctt ttaatgtaga aacttatatt gaaggaggcg
    12001 agaatcatgc gagaagaaaa aagtaatgaa aagtatgatt gttattggtg taatcaagag
    12061 aataatttct gtgtagaaat aaaagataat atagtcatga tagatgatgg cactggtacg
    12121 ttaaaacaag ccgttttcat agggtataaa caaatccaaa ttaatttaaa ctgttcacat
    12181 tgtcaaaact tgaatagaat aaaattaaat ttgtagaatt ttaaaatgga aattattgaa
    12241 ggaattaaaa atagaaagtg ttaatattta gactgaaaat tatattttat acaataaaag
    12301 tttatttaaa taacattgta ttttaatatt tattttcagt cttactttat taaaattatt
    12361 ttttggttat gtctggaaaa tatctgtcta ctaatttttc tgaaatattt tttaattggt
    12421 cagccattga agtcagactt aataaggcta tcaataaatc atctttattt tctggattta
    12481 tactaatatt ttcaaaatta gactctataa agtccggtat attctttctt tgattttctt
    12541 ttattaacat gaatttttcg taacatgaaa ctaaatcttt ttcactgaaa ttatcctttt
    12601 catacacacc atgtaagatt cttttaattt cttgtattgt aagtgtattt ttcatagaat
    12661 aaataattat catctgtaaa atttgttctt tagtatactt ttttccctta actggtttga
    12721 ttaatccctc tttgctgtaa ttatttatca tggttttagt aagaatttta tcactttcaa
    12781 atcttttatt ggcagaaagc ttattgtcaa ttagagaaat aatttgatcc atatataagt
    12841 caatagatgg aatatcgttt gaagttatat ctgcattatt tagtgtttct agtattatag
    12901 tattaagttc attattcatc atatgttacc tccataatat atataataca ctaaattaga
    12961 aagtaaaaca atattaaggc ttgtaaaaaa aacataaagt ggtataatta aatatataat
    13021 atagtaatgg aaactatatt tgtgaatctg atttttaaaa tacataattg taggaggacg
    13081 aaatgtatca atttttttta aagggtagag acccaataag cagtttaact cattttatag
    13141 gtgcttgttt atctctttta gcaactataa ttttagtttt tcaatctgtt acattacagg
    13201 aaacttctct cttgatgata gtatctgtat cggtatttgg attttcacta attgctttat
    13261 atagcgcaag ttcgtattat cattttttaa aggggacacc agaacaagaa ttgtttttta
    13321 gaaaagtaga ccatgcaatg atttacgtat tgattgctgg ctcttataca ccaatatgtc
    13381 tgaattttat ggaaaaaaaa gaaggcataa tatttgttac agcaatatgg atagttgcat
    13441 ttattggtat aataataaaa atattttgga tggatgcacc tagatggtta tctaccagta
    13501 tatatttact tatggggtgg gcaattgttt ttgatataaa tgcattcaat tcaattccta
    13561 aggattgcct tagactatta attatggaag gtgtatctta ctctataggt gcaattattt
    13621 atataattaa aaagcctaat ataagtcctg agtttggatt tcatgaaata tttcatattt
    13681 ttattatgat aggttcttta tttcactttt tagcagtttt attgtatgtt ttatagtttt
    13741 aaaagatttg attaattgat actaaaattc tacaatgaat aaaatggaac tgccttatat
    13801 agattatatc ttcgacaata agacagttcc ttattataaa tattaaattc tacttataat
    13861 aaaattttga taaaaatagt ccattcaaaa tgcctattcc ataattcctt taatgtactt
    13921 ttcatttacg acatatagtt tgaatttatg tactataatt atatcctcta aattctctga
    13981 aacactaaca attctgacac atttgtttct aatttctaca ggtaaatcta aatcatttgt
    14041 taaattcaca tagtcatcat atttatacat aaaatcatcc ctttacatta agat
//
LOCUS       DAMJYR010000096        13988 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.099, whole genome shotgun sequence.
ACCESSION   DAMJYR010000096 DAMJYR010000000
VERSION     DAMJYR010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13988)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13988)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13988
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.099"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(<1..184)
                     /locus_tag="PC471_002749"
     CDS             complement(<1..184)
                     /locus_tag="PC471_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF2789863.1"
                     /translation="MAVVEKAYKFRMYPNKKQQELINKTFGCCRFVYNKYLAKRIEVY
                     KNDKETFTYKQCSSDLT"
     gene            complement(186..593)
                     /gene="tnpA"
                     /locus_tag="PC471_002750"
     CDS             complement(186..593)
                     /gene="tnpA"
                     /locus_tag="PC471_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013824389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HDF2789864.1"
                     /translation="MEVTHGRGYVYLVQYHIVWCVKYRHPVLTNEIEEDLINILNKIA
                     EDNKFQILECNSDKDHIHLLINCSPQHYIPDIIKALKGVSARLLMKKHGEQLKKKLWG
                     GHLWNPSYFVATVSENTEEQIRKYINNQKKQVK"
     gene            complement(1162..1371)
                     /locus_tag="PC471_002751"
                     /pseudo
     CDS             complement(1162..1371)
                     /locus_tag="PC471_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861712.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4004 family protein"
     gene            complement(1473..2021)
                     /locus_tag="PC471_002752"
                     /pseudo
     CDS             complement(1473..2021)
                     /locus_tag="PC471_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426706.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2380..4290)
                     /gene="phnW"
                     /locus_tag="PC471_002753"
     CDS             complement(2380..4290)
                     /gene="phnW"
                     /locus_tag="PC471_002753"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HDF2789865.1"
                     /translation="MKKIYGEKIKAVVFDWAGTTVDYGCFAPLNVFIEIFKRRGIDVT
                     MEEARKPMGKLKIDHIREMCEMDRIKNIWSDKFGKVPTEDDVNELYAEFEPMLFETLE
                     EYTTPIPHVVETIEKLRKNGLKIGSTTGYTREMMNIVEPNAAKKGYSPDFLVTPSEVS
                     QGRPYPWMCYKNAEALGVSPMSSMVKVGDTISDVKEGVNAGMWSVAVIKGSSELGLTQ
                     EEVENMDKEELKAKMSIVSKKFKEAGAHFVIETMAELEDILIKIENETIKSDFVPEND
                     YILLTPGPLSTTKSVRASMLKDWCTWDVEYNNLVQDVRRRLVSLATQNTDKYTSVLMQ
                     GSGTFSVEAIIGSTISKDGKLLVIANGAYGKRMKDICNYLNIQFVDCTFKDIEAVDLN
                     VVENLLKENKDITHISMVHCETTTGRLNPIQEVGKLAKEYNKIYIVDAMSSFGGIEID
                     VEDFNIDFLVSSSNKCIQGVPGFGFIIANKEKLSKCKGIAKSLSLDVYAQWETMEKNN
                     GKWRFTSPTHVVRAFYQALLELEEEGSVEKRYARYKENQFTIASRLKSLGFDTLVNDN
                     AQSPVITTFLYPKNAKFEFMEFYTYLKDNGFVIYPGKLTDIDTFRIGSIGEVYPTDME
                     RLADVIEKFINR"
     gene            complement(4569..5354)
                     /locus_tag="PC471_002754"
     CDS             complement(4569..5354)
                     /locus_tag="PC471_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HDF2789866.1"
                     /translation="MSLNSIDRKKEILKLLDSKGKVNTKELVKRLEVSSETIRRYLEE
                     LENENKLKKVYGGAIKINYGVIEPEHIKRNNTNLDKKQSIAKYAAKFVNDNESIIIDE
                     GTTNLQIVDYIISVNNLKIITNSYPVLSKLISYENQGLFDGEVIFIGGKVNLKHQRTA
                     CPMSIDTMKNLYVDKAFISSEGILDSFGISSIDQNKALLSKEYIKNSKESFVLCDSSK
                     IGIASMYKIADLEELDFVICDVEPPNEWKDKLNEINITWIEAK"
     gene            complement(5571..5801)
                     /gene="dltC"
                     /locus_tag="PC471_002755"
     CDS             complement(5571..5801)
                     /gene="dltC"
                     /locus_tag="PC471_002755"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HDF2789867.1"
                     /translation="MQETVIEIFEDVLGTDEIREDLDLNLFETELLDSLAIIEVLLEI
                     ENRLGIELQPTDLERKDMSTVNNLVKFLETRK"
     gene            complement(5820..6968)
                     /gene="dltB"
                     /locus_tag="PC471_002756"
     CDS             complement(5820..6968)
                     /gene="dltB"
                     /locus_tag="PC471_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HDF2789868.1"
                     /translation="MIFSQYGDYFYLYILLLTSIPAVILGLMGKNIKYYGMLASLFMI
                     FLIVGIDVQLKYLVIFIILEVIIVKGYEYVRRKTKNKYIYWGFLFASMLPIIINKISP
                     VTSFGIIGFIGISYLNFRTIQMVIEIYDGAIKEVKISKMLYFMLFFPTLSSGPIDRSR
                     RFEQDLEKQISRKEYIEEYLLPGIKNIVMGVGYKFVIAFLINTYWVSRIPKDMSFINI
                     LSYMYAYSLYLFFDFAGYSLFAIGTGYIFGIQVPINFDKPFISKDMKEFWTRWHISLS
                     RWFGDYIFSRFVMSSMRKKRFKKRTTAAHVAQMITMITMGFWHGLTWYYVAYGVYQGL
                     ALVLTDIYQRKSKFYKKHKKDKWFERVQIFITFHIVCFGLLIFSGYLA"
     gene            complement(6968..8482)
                     /locus_tag="PC471_002757"
     CDS             complement(6968..8482)
                     /locus_tag="PC471_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase"
                     /protein_id="HDF2789869.1"
                     /translation="MKIIEGIKKYSNTDRTALMCNGDKLSYKDLNEYSDAISVFLKDV
                     YKEEDTPIVIYGNKENMIMACMIGALKSGRAYVPLDISFPIDRVFEVTKEIKPKVLFN
                     FSDEKDFGDINVIDMDKLNYIINEYQGKSLDKENWVKDDENAYILFTSGSTGKPKGVQ
                     ISSNNLDSFSDWISPYLNIDGSEKVIMNQPAYSFDLSVTTIYPGLIHGATLFSISKDV
                     LADYKELFRQFSISDIAVWVSTPSFAGVCITEKEFNSKMLPNLESMIFIGEALSKNLT
                     KELMSRFPNTRIINGYGPTEATVGVSVNDMTQKAIDDEKSLPVGYPMSNCKIKILDED
                     GNELKENEKGEIIIIGPSVSKGYFNNKEKTDEVFFYDEIDGVKWRAYKTGDMGYLLDG
                     NIYYCGRKDFQIKLNGFRIEIEDIENNLRKVHNVKNAVVLPVYKDEKIAYLKGIVELN
                     EKNDLSNIKNGMIIKKELGKYIPSYMIPRNISIISEFPTNINGKIDRKKLMEEI"
     gene            complement(8603..9793)
                     /gene="dltD"
                     /locus_tag="PC471_002758"
     CDS             complement(8603..9793)
                     /gene="dltD"
                     /locus_tag="PC471_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HDF2789870.1"
                     /translation="MRKLIYFITPFIIGVVFLFGLDKFLDSKTDELLREKNLLPIMDD
                     TLSDIKDKGVTANNHFLREKDIMILGSSELSNSTKQHPKYYFNTNRSKNKVFAIGRAY
                     TQTLQDAAILGSMNPNIDNKKVVLLISMQWFMEKDGVTSHHYQSRFSPIQFYRFLDNP
                     KISKQNKIEYAKKSSKLLWGSDEYKAEALYAKLYEPKTFPEKAEKVLLEPYFQGRKYC
                     IALKEKGILYKRLIKLDKKRATKRKRPINWSYERKKAIEDAKKRVGKNPLNIDKYYYK
                     EHFKDGIEQYKGRDKDVNLLTSKEFENYKLMLNVCTDLGIKPVVVLIPSMDKFYNLTG
                     ISEKERNQYYDKAQKIAESKGFEVLNLKDKGSDKYYLRDVMHLGTKGWVDVCERLFKI
                     FKEQ"
     gene            complement(10081..11100)
                     /locus_tag="PC471_002759"
     CDS             complement(10081..11100)
                     /locus_tag="PC471_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDF2789871.1"
                     /translation="MMGKIRFGIVGTSNIANIFLRAASRVKDFELVAVYSRNLEKARE
                     FGTLYGASIFFDDLEQMAKSKDIDAVYIASPNALHSKQTITCLKHKKHVLCEKSLASN
                     LKEVKSMIKTAKDNNVLLMEAMRITCVPNFKAVKENLYKIGKIRRFFGSYCQYSSRYD
                     KYKNGIIENAFKKELSNGALMDIGVYCIHPMVNLFGAPKTVKAVSHILQTGVDGEGSA
                     VFQYDDMDAIIQYSKVADSYIPSEIQGEEGSIIIEKLNLFEKAIIKYRDGREEDISIP
                     KEKAEEKPREIEGMYYELVEFISLINNNKIESNINSHENSIIVMEIMDEIRRQSSIVY
                     PADSI"
     gene            complement(11276..11815)
                     /locus_tag="PC471_002760"
     CDS             complement(11276..11815)
                     /locus_tag="PC471_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter permease"
                     /protein_id="HDF2789872.1"
                     /translation="MDNVGSLLYFLFNFFLYGFIGWIIENLYSYYKQGHFQEDGFLNG
                     PFKPMYAIAMSIIIEVSRFIDPNNIFALLLLCLVVPTVVEYITGILMKVYFNKVYWDY
                     SDYKYNSRGIVCLKFSVYWTILTFIGVRYFQVYIVDSLYSLIIPYWPIVVTILLVTLF
                     VDEILTIKFLRGNKDIVER"
     gene            complement(11916..12596)
                     /locus_tag="PC471_002761"
     CDS             complement(11916..12596)
                     /locus_tag="PC471_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HDF2789873.1"
                     /translation="MQKVEGIIFDMDGVLFDSERISLEFWMETFEKYGYTMTKEIYTS
                     VMGRNRKGIIEGLTDIYDSSVPIIDLYDEKTKNMIEFMERKGAPIKLGVNELISFLKE
                     NGYKMAVATSTKRERAVKRLAKANLKDYFDAIVCGDDVVNSKPNPEIFLKAAKKINVN
                     PQNCIVIEDSPMGVEAAYNGGIRCINVPDLKEPDEQIKSQSHKILENLLEVREYLKSL
                     NSKECHNL"
     gene            complement(12625..13914)
                     /locus_tag="PC471_002762"
     CDS             complement(12625..13914)
                     /locus_tag="PC471_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HDF2789874.1"
                     /translation="MRELLKVTLIVMLVLINAFFVACEFAMVKLRSSRIDTMITEGNN
                     NAKRCKIIKDNLNSYLSACQLGITLCSLALGWMGESTVKELILPIVSVFNLSESIIYT
                     ISISISFLIITMMEVVIGELVPKALALYNTEKIMLSTSFLLVGFYKLTYPIIYFFNLS
                     TDLFLKPFGYSQADEVDEPHTGDEIRLLVEESYKSGLIDESEQRLVDNIFEFEEKKIR
                     EIMVPRTDMVCIYESDSEEKILAILKEEGVTRYPVCRKNKDDILGFVHIRDLYNQKIN
                     ENKIELEEILRDIIYISENLTIDKALERIRKEKLQLAIVVDEYGGTSGVVTIEDILEE
                     IVGEIQDEFDKEETHIKKIGAGSYLVDGTESINYINKYFGLDIEHDGFDSIGGWISYM
                     LGSNIKVNQSVTFENYNFTILELDKLRVVKLIIKSVI"
BASE COUNT         4462 a         2525 c         1367 g         5634 t
ORIGIN      
        1 tggttaaatc agaactacac tgtttataag taaaagtttc tttatcgttt ttataaactt
       61 ctattctttt agcaagatac ttattatata caaatctaca acatccaaaa gttttgttaa
      121 ttaactcttg ttgtttttta tttggataca ttctaaactt ataagctttt tccactactg
      181 ccacctcact ttacttgttt tttctgatta ttaatatatt ttcttatctg ttcttctgta
      241 ttttctgata cagtagcaac gaaataacta ggattccaaa gatgtcctcc ccataatttc
      301 tttttcaact gttctccatg ttttttcatt aacagccttg ctgatacacc ttttaatgct
      361 tttattatat ctggtatata gtgttgtggt gaacaattta ttagtaaatg tatgtgatct
      421 ttatcactat tacattctaa tatttgaaat ttattatctt cagctatttt gtttaatata
      481 ttaattaaat cttcttcaat ttcatttgtt aatactggat gtctatattt tacacaccat
      541 actatatggt attgtactaa atacacatac cctctaccat gagtcacttc catttttcat
      601 cacccaacat aatattaaca tagtaattac ttaaaacaag tattcttaag gtttttatat
      661 ttatttttaa ataacatacg taagaaacgc aaatcttaac ttttatcatt tttgaggttg
      721 tcgttcacat aagttcgcta ctacctatgc agttctctta tgaacttctt acactttcat
      781 gcaagcacag actatatctt atccttcggc attatccgtt aaggtctacc cacttccact
      841 accgatagct tgtagtgtac ttccctcaag aggaatagtc gttgaagttt ttcctattcg
      901 gagcttacct gctgattgcc cattttaaag tacttaggat ttaaccttat accatctaac
      961 caattttttc tactttcgta actttcacac ttaggtttat ttcatcctta tgttttagtt
     1021 tggttagctt taggggtttc cagcaattca agtagtattg gataggttct atcctatctc
     1081 tacatataag tttccctata tgctgactat tttagctact aactcatgac taaagtcaca
     1141 agtgtgcata gctatttttt aatcaagaga agtctcttct ttcatagaca atattaactc
     1201 tattctttct agtattttat ctcttggaaa gaaagtctct tgacctgtaa aagctgattt
     1261 ttttataaac cattcctctg gtattatgtt ttttcttttc catctatata attgtccata
     1321 agatatatta gttttgctca ataaatcttt cttagatatt aattcttcca tagctcttct
     1381 agcccacctt tatactgttt tttacagtag aattatctga aactgataaa ttatcactgt
     1441 attcaatatt tccaacatca taattatcta ctagagatat gctttttttc tagcatacga
     1501 aattttttgc aataatctcc atgccccttt tttatattta tactacttgc tccaacttca
     1561 ttaaaattag aagttccatt taaactaata ttaacttctt cacaattcaa aaatccatta
     1621 cactccaaga ttccacttaa attgatattt tcacaatcta tattctcatt tgcttttaaa
     1681 tatccttctc catgaataat atccgatttt atacttcctt ctacattaat aattccacta
     1741 gtatcaattt caacagaatt aatatttcct gtacatctaa gaagtcccga tatctcaact
     1801 tttgatgcca gtatgtttcc ttctacttta cttaaaccag atgtaaaaaa ttcattacat
     1861 tctgttgtcc caaaaacttt tgctgaacca gaagaatcaa atttactaca ttttacactt
     1921 ccttctattt tgcctgttcc tgatatatta acattatcaa aaattccacc tttacatttt
     1981 ccaacaccat caaatcttag tgaacttaat tcttcattca taatgatatc ctttctttac
     2041 cgtattttat ctttaaacct aatataacaa aacattgtta cgttgtcaag tatgataata
     2101 atacttaata aagcaactat catttatttt ctataaatag gcataaaaat aatagggaaa
     2161 tgctttattt aaactataaa tctaatttct tagattgtgg tttaaataaa atatatccct
     2221 attactcaat gtttatatat tcattgtata aacttctaaa atataagtct ttaaattcta
     2281 ctgtcaatac tttttataca ctgtttgtgc tatcatctca cacacctaaa ggagtaggct
     2341 ttgcgcacta ttctgtaacc tacaagacac tatcttcttc tatctattga taaatttttc
     2401 tattacatca gctagtctct ccatatcagt tggatagact tcacctatgc tacctattct
     2461 aaatgtatct atatcagtca atttacctgg atagataaca aatccattat cttttaaata
     2521 agtataaaat tccataaatt caaactttgc atttttagga tataagaaag ttgttattac
     2581 aggtgattga gcattatcat tcaccaaagt atcaaagcct aaagatttta atcttgaagc
     2641 tattgtaaac tgattttctt tatatctagc atatcttttt tcaacagaac cttcttcttc
     2701 caattctaat aaagcttgat aaaaagctct tacaacatgt gtaggtgaag taaatctcca
     2761 tttaccatta tttttctcca tagtctccca ttgcgcatac acatcaagag ataatgattt
     2821 agctatacct ttacatttag ataatttctc tttattagct attataaaac caaatccagg
     2881 tactccttgt atacatttgt tagaactact aactaaaaaa tctatattaa aatcttcaac
     2941 atcaatctca attcccccaa aactactcat agcatcaact atataaattt tgttatattc
     3001 ttttgcaagt ttccctacct cttgtatagg atttaatcta cctgtagtag tttcacagtg
     3061 aaccattgat atatgtgtta tatccttgtt ttcttttaat aaattttcta ctacatttaa
     3121 atccactgct tctatatctt taaatgtgca gtcaacaaat tgtatgttaa gataattaca
     3181 tatatctttc attctctttc catatgctcc atttgcaatt acaagaagtt taccatcttt
     3241 tgaaatagta cttcctatta tagcttctac tgaaaaagtc ccacttcctt gcattaatac
     3301 acttgtgtat ttatctgtat tttgggttgc tagagatact aatcttcttc taacatcttg
     3361 tactaggtta ttatattcaa catcccaagt acaccaatct tttaacatag atgctcttac
     3421 tgatttagta gtagatagtg gtcctggtgt aagtaatatg taatcatttt ctggtacaaa
     3481 atctgattta atagtttcat tctctatttt tattagtata tcttctaact cagccatagt
     3541 ttctattaca aaatgtgcac ctgcctcttt aaatttcttt gaaactatac tcatcttagc
     3601 ttttaattct tccttatcca tgttctctac ttcttcttgt gtaagcccta actcacttga
     3661 accttttatt acagcaacac tccacattcc tgcatttacg ccttctttta catcacttat
     3721 tgtatctcct acttttacca tagaactcat aggagacacc cctaaagctt cagcattttt
     3781 atagcacatc caaggatacg gtcttccttg agatacttct gatggtgtta caagaaaatc
     3841 gggtgagtat cctttctttg ctgcatttgg ttcaactatg ttcatcatct ctcttgtata
     3901 acccgttgta gaacctattt ttaatccatt ttttctcaac ttttcaatag tttcaacaac
     3961 atgaggaatt ggagtcgtat actcttctaa tgtctcaaat aacattggct caaattcagc
     4021 ataaagttca tttacatcat cttcagttgg cactttacca aacttatctg accaaatatt
     4081 ctttattcta tccatttcac acatttctct tatatggtct atttttaatt ttcccatagg
     4141 ttttcttgct tcttccattg ttacatctat acctcttctt ttaaatatct ctataaatac
     4201 attaagaggt gcaaaacatc cataatctac tgttgttcca gcccaatcga atactactgc
     4261 ttttattttt tctccatata tctttttcat atttatagcc tccatagatt ctttttagaa
     4321 tttttgtgtt tttaattgtt tttactttta aagtataact ttcatatgtt aattatacat
     4381 catatttagg ttaaatatat attaaatttc tgtttttagt tgtatttagt tgtttttatt
     4441 ttgtatcata ttcccataaa atcctaagtt acaatctaaa atttactttc taatcttaat
     4501 ctatgaactg actatatttt atgaatttag ttgtattgag catagttgta ttatataaaa
     4561 ctaagggttt attttgcttc tatccatgtt atatttatct catttaactt atccttccat
     4621 tcattcggag gttctacatc acaaataaca aaatctaatt cctctaaatc tgctatctta
     4681 tacatactag caattcctat tttagaacta tcacataata caaaactttc ctttgaattt
     4741 tttatatatt cttttgacaa caatgcctta ttttggtcta tactactaat tccaaaacta
     4801 tcaagtatac cttcactaga tataaaagct ttatctacgt ataagttttt catagtatct
     4861 attgacattg gacatgctgt cctttggtgt tttaaattta cttttccacc aataaatata
     4921 acttctccat caaacaaacc ttgattctca taagaaatca attttgatag aactggataa
     4981 gagtttgtaa taatttttaa attgtttaca gatattatat aatctacaat ctgcaaattg
     5041 gtagtacctt catctatgat aattgactca ttgtcattta caaacttagc tgcatattta
     5101 gcaatacttt gctttttatc taggttagta ttgtttctct ttatatgttc tggttcaatc
     5161 actccataat ttatttttat agcaccacca tatacctttt ttaatttatt ttcattttct
     5221 aattcttcca aatatcttct tattgtttct gatgatactt ctaatctttt tactaattct
     5281 ttagtgttta ccttcccttt tgaatctaat aatttaagta tttctttttt tctatctatc
     5341 gaatttaaag acattcattt tcctcctaaa tataatatat ttttctccta taatattaca
     5401 atgataccta agatttatga aatcatattt atgtattaaa ttttttactt aattaaagtt
     5461 attttcaaaa gactttgtat tatcattaaa aaagtccatc aaatattagt atcagatgga
     5521 ctttgtaaaa ttttatttta taaattaata tttaaagtct aactactaat ttattttctt
     5581 gtctccaaga attttactaa attatttaca gtagacatat cttttctctc taaatcagtt
     5641 ggttgaagct ctatccctaa tctattttca atttcaagta aaacttcaat tatagctaat
     5701 gagtctaaaa gttccgtctc aaataaatta aggtctaaat cttctcttat ctcatcagtc
     5761 cctaatacat cttcaaatat ttctataact gtttcttgca taaattttcc tccttaaaat
     5821 taagctaaat aacctgagaa tatcagcaat ccaaaacaca caatatggaa tgtaatgaaa
     5881 atctgcactc tctcgaacca tttatctttt ttatgttttt tataaaattt agattttcgc
     5941 tgatatatat cagttaacac aagtgccaat ccttggtata caccataagc aacataatac
     6001 caagttagac catgccaaaa tcccattgtt atcatggtta tcatctgagc cacatgagca
     6061 gcagtagtcc ttttcttaaa tcttttcttt ctcattgatg acataacaaa tctagaaaaa
     6121 atataatctc caaaccatct tgaaagactt atatgccatc ttgtccagaa ctctttcata
     6181 tctttactga taaatggctt gtcaaagttt ataggtactt gtattccaaa tatgtatcct
     6241 gtacctatag caaataaact atatcctgca aaatcaaaga acaggtataa actatatgcg
     6301 tacatataac ttaaaatatt tataaaactc atatctttag gtattcttga tacccaatat
     6361 gtatttatca aaaatgctat aacaaactta taacctactc ccatgacaat attttttatt
     6421 cctggcagta agtattcttc tatatactct tttctagaga tttgtttttc caagtcctgt
     6481 tcaaatcttc ttgacctatc aataggtcca gaacttaaag ttggaaaaaa taacataaaa
     6541 taaagcatct ttgaaatttt aacttctttt atagctccat cataaatttc aataaccatt
     6601 tgtatagttc tgaaatttaa gtatgatatt ccaataaatc caattattcc aaaagaagta
     6661 actggagata ttttatttat tattattggg agcattgatg caaacaagaa tccccaatat
     6721 atgtatttat ttttagtttt tcttcttaca tattcatatc ctttaactat tattacttca
     6781 agaataataa atattaccaa gtactttagt tggacatcaa ttcctactat taagaatatc
     6841 ataaacaaac ttgccaacat accataatac tttatattct tgcccattag acctaaaatt
     6901 actgctggta ttgatgttaa taataatata taaagataaa aataatctcc atattgtgaa
     6961 aaaatcatta aatttcctcc ataagtttct ttctgtctat tttaccattg atatttgttg
     7021 gaaactcaga gattattgaa atatttcttg gaatcatata tgaaggtata tactttccaa
     7081 gttccttttt tattatcatc ccatttttaa tattacttaa atcattcttt tcatttaact
     7141 caacaatacc ttttaagtaa gcaatttttt cgtccttata tactggtaaa actactgcat
     7201 tttttacatt atgtactttt ctcaaattat tttctatatc ttctatttca attctaaatc
     7261 catttaattt aatctgaaaa tcttttcttc cacaataata tatatttcca tctaaaagat
     7321 atcccatatc tccagtttta taagctctcc actttacacc atctatttca tcatagaaaa
     7381 atacttcatc agtcttttct ttattattga agtaaccttt agaaactgat ggtccaatta
     7441 ttataatttc acccttttca ttttctttaa gctcatttcc atcttcatca agtattttta
     7501 ttttacaatt tgacataggg tatccaactg gaagactttt ttcatcatct atagcctttt
     7561 gagtcatatc atttacactt acaccaacag tagcctcggt tggtccatat ccattgataa
     7621 ttcttgtgtt tggaaatcta ctcataagtt ctttagtaag attttttgaa agtgcttctc
     7681 ctatgaatat cattgactca aggttaggaa gcattttact attaaactct ttttcagtta
     7741 tacatactcc tgcaaaagaa ggagttgaca cccatacagc tatatctgag atactaaatt
     7801 gtctaaatag ttctttatag tctgctaata catcttttga tattgaaaat aatgtagctc
     7861 catggataag accagggtat attgttgtaa ctgaaaggtc aaaagaatat gctggttggt
     7921 tcattattac tttttcactt ccatctatgt ttagatatgg gcttatccaa tctgaaaagc
     7981 tatctaaatt gtttgaactt atttgaaccc cttttggctt tcctgtactt cctgaagtaa
     8041 atagtatata tgcattttca tcatctttta cccagttttc tttatctaat gattttccct
     8101 gatattcatt tattatataa tttaatttat ccatatcaat tacattgata tctccaaaat
     8161 ctttttcatc actaaagtta aataatacct ttggttttat ctctttagtg acttcaaaaa
     8221 ctctatcaat tggaaaacta atatcaagag gcacatacgc tctacctgat tttaaagctc
     8281 ctatcataca tgccattatc atgttttctt tatttccata aattactata ggagtatctt
     8341 cttctttgta tacatccttt agaaatactg atatagcatc tgaatattca tttaaatctt
     8401 tataagaaag tttatctcca ttacacatta gtgctgttct atctgtattt gaatattttt
     8461 ttattccttc aataattttc atataagaat gctcctttaa aatccgttat aaataaatgg
     8521 caattcttta aatggagtaa atgttagaac tcctatcgcc aatattataa ccatagttaa
     8581 acaaatacta gttaacaaat ctttattgtt ctttaaatat tttaaataat ctctcacaga
     8641 catctaccca tcctttcgta ccaagatgca ttacatccct taaataatac ttatctgacc
     8701 ctttatcttt aaggtttaga acttcaaagc ctttgctttc tgctattttt tgtgctttat
     8761 cgtagtactg atttctttct ttttcagaaa taccagtcaa attatagaat ttatccatac
     8821 ttgggattaa tacaacaact ggcttaatac ctaagtcagt acatacattt agcatcagtt
     8881 tataattttc aaactcctta gatgttaata aatttacatc tttgtctctt cctttgtatt
     8941 gctctatacc atctttaaaa tgctctttat aatagtactt atcaatattt agtggatttt
     9001 taccaactct ttttttagca tcttcaatgg ctttctttct ctcatatgac caatttattg
     9061 gtctttttct ttttgttgct ctctttttat ctagcttaat taatcgttta taaagaatac
     9121 ccttttcttt aagagctata caatactttc ttccttgaaa ataaggctct aaaagcacct
     9181 tttcagcttt ttcaggaaat gtcttaggct catatagctt agcatataat gcttctgctt
     9241 tatactcgtc acttccccat aataacttac ttgatttttt tgcatattca atcttatttt
     9301 gtttagatat ttttggatta tctaaaaatc gataaaattg tattggtgaa aatctagact
     9361 gataatggtg acttgtgact ccgtcttttt ccataaacca ttgcatagaa attaacaaga
     9421 caactttctt attatcaata ttaggattca tacttcctaa tattgcagca tcttgtaatg
     9481 tctgagtata ggctctccca attgcaaaca ccttattttt actcctattt gtattaaaat
     9541 agtactttgg atgttgtttt gttgaattac taagttctga tgaccccaaa atcataatat
     9601 ccttttctct taaaaaatga ttatttgctg taaccccttt atcttttata tcacttaaag
     9661 tatcatccat aattggaagt agatttttct ccctcaaaag ttcatcagtt ttagaatcaa
     9721 gaaatttgtc taacccaaat aaaaatacta cgcctattat aaaaggtgta ataaagtata
     9781 tcaattttct catattttct ctcctctaaa aatattcaaa tttatgttat tattaatcat
     9841 attagttttg atatgtatat tcaactacaa aattgtaata aaatcgtcaa aacttttttt
     9901 gatactgtta caaaattgta aaaaaaaaga aaaaaacgcc aaaaataaat ttctttttag
     9961 cactttttca tacttataat ttgacatttt aacacttggt taagtataca ccttacctta
    10021 acttaaaatt aaatttattt aagaatatat attataaatt cttatatttt ctaatataaa
    10081 ctatatacta tcagctggat aaacaatact actttgtctt cttatctcat ccataatttc
    10141 cattactatt attgaatttt catgagaatt tatatttgat tctattttat tgttatttat
    10201 aagacttata aactctacaa gttcataata cattccttct atttctctag gcttttcctc
    10261 tgctttttct tttggaatag atatatcttc ttctcttcca tctctgtatt taataatagc
    10321 tttttcaaat agattaagct tttcaattat tatacttccc tcttctcctt gtatttcaga
    10381 tggtatataa gaatcagcta ctttagaata ctgaattatc gcatccatat catcatattg
    10441 aaatacagct gaaccttctc catccactcc agtttgaagt atatgagaaa ctgcctttac
    10501 agtttttgga gcaccaaata aattcaccat aggatgtata caatacactc ctatatccat
    10561 taaagctcca tttgacaact cttttttaaa ggcattttct ataattccat ttttatattt
    10621 atcatatctt gaagaatact gacaatagct tccaaaaaat cttcttattt tccctatttt
    10681 atataagttt tctttaactg ctttaaagtt tggaacacat gtaattctca tagcttccat
    10741 aagcaataca ttattatctt tggctgtttt tatcattgat ttgacttctt ttaaattaga
    10801 tgctaacgac ttttcacaaa gcacatgttt tttgtgtttt aaacaagtaa tggtttgctt
    10861 tgaatgtaat gcatttggag aagctatgta tacagcatct atatccttcg atttagccat
    10921 ttgttctaaa tcatcaaaaa aaatacttgc tccatataga gttccaaatt ctctagcttt
    10981 ttctaaattt ctagaatata cagcaaccaa ttcaaagtct ttaaccctag atgcagctct
    11041 tagaaatata tttgctatat tgcttgtccc aacaatacca aatcttattt tacccatcat
    11101 aaatcactcc ccattttcta cactttattt taattttaat aaaaaacttt acaacttata
    11161 gatatattat aaaataattt tatcaaataa aaaagaaagc taattttaaa tgatttcacc
    11221 attttaacat agctttcttt taactacaat ctaaattcaa cttatatttt taaagttatc
    11281 tttctactat atctttattt cccctcaaaa attttatagt aagaatttca tccacaaaca
    11341 atgttactaa aagtatagtt acaacaattg gccaataagg tattataaga ctatataaac
    11401 tatcaactat atacacttga aaatatctaa ctccaataaa tgtcaatata gtccaatata
    11461 cagaaaactt tagacatact attcctctac tattgtactt ataatctgaa taatcccaat
    11521 agaccttatt aaaatatact ttcataagta ttcctgtaat atattctact actgtcggca
    11581 ctactagaca taataaaagc aatgcaaata tattattggg gtcaataaat cttgagacct
    11641 ctattataat cgacattgca attgcataca ttggtttaaa tggaccattt aaaaatccat
    11701 cttcttgaaa atgcccttgt ttataataac tatataagtt ttcaattatc caacctataa
    11761 aaccatacaa gaaaaaatta aataaaaaat ataatagaga acctacatta tccatgaata
    11821 ttcctcctag ctgtttatac ttattttttc tcatattcat ggatttattc taaatcaaag
    11881 aaattatatt taatttattt aattaatact ttatattata gattgtgaca ttctttagag
    11941 ttcaaacttt ttaggtattc tctaacttcc agtaaatttt caagtatctt atgtgattgt
    12001 gatttaattt gctcatctgg ctcttttaaa tctggtacat ttatacatct tataccacca
    12061 ttgtatgcag cttcaactcc cataggcgaa tcctctatga caatacaatt ttggggatta
    12121 acatttatct ttttagctgc ttttaagaat atctcagggt ttggtttaga gtttacaaca
    12181 tcatctccac aaactatagc atcaaagtaa tcttttaaat ttgccttagc taatctcttt
    12241 actgctcttt ctctctttgt tgatgttgca acagccattt tgtatccatt ttcctttaaa
    12301 aaagagatta gttcattgac tcctaatttg ataggagctc ccttcctctc cataaattca
    12361 atcatatttt tagttttttc gtcatataaa tctattattg gtacagaact atcgtatata
    12421 tctgtaagac cttctattat tcctttacga tttcttccca taactgatgt ataaatttcc
    12481 ttagtcatag tatatccata cttttcaaac gtctccatcc aaaattcaag agaaattctc
    12541 tctgagtcaa acaaaactcc atccatgtca aaaataattc cttctacttt ttgcatacaa
    12601 ttcctccaat attttttgat ataattatat tacacttttt atgattaatt ttacaaccct
    12661 caatttatcc aattctaata ttgtaaaatt gtaattttca aaagttacac tttggtttac
    12721 tttaatattt gaaccaagca tataggatat ccacccacct atactgtcaa atccatcatg
    12781 ctctatatca agtccaaaat acttattaat atagttgata gattcagttc catcaactaa
    12841 gtaagaacct gctcctattt tcttaatatg agtctcttct ttgtcaaatt catcttgaat
    12901 ttctccaact atctcttcta gtatatcttc tattgttacc acaccagatg taccaccata
    12961 ttcatccaca actatagcaa gttgaagttt ttcctttctt attctctcaa gagctttatc
    13021 tatagttaaa ttttcagata tatatataat atctctcaat atttcttcta gttcaatttt
    13081 attttcattt atcttttgat tgtataaatc tcttatatgt acaaaaccta atatatcatc
    13141 tttatttttt ctgcatactg gatatcttgt aacaccttcc tcttttaaaa tagctaatat
    13201 cttttcttca gaatcacttt catatataca taccatatca gttctaggaa ccattatctc
    13261 tctaattttc ttttcttcaa attcaaaaat attatcaact aatctttgct ctgattcatc
    13321 tataagtcca cttttataac tttcttctac taatagtctt atttcgtcac ctgtatgggg
    13381 ttcatcaacc tcatcagctt gtgaatatcc aaatggttta agaaataaat cagtacttaa
    13441 gttgaaaaaa tatattattg gatatgttaa cttataaaat cctactaata aaaaagatgt
    13501 actcaacatt attttttctg tattataaag tgctaaagct ttaggaacca actctcctat
    13561 tacaacctcc atcattgtta taattaaaaa agaaatggaa attgatatag tatatataat
    13621 actttcacta agattaaata cacttactat tggtaaaatt aactctttaa ctgtagattc
    13681 tcccatccaa cctaatgcaa gtgaacaaag tgttatacca agttgacatg cagaaagata
    13741 ggaatttaag ttgtctttta ttattttaca tctttttgca ttattattac cttcagttat
    13801 catggtatca atcctagaac tacgaagttt taccatagca aattcacaag ctacaaaaaa
    13861 agcatttatc aaaacaagca ttactataag tgttactttt aataactccc gcataaagta
    13921 ttggttctta taatgtaaga aatctataag aactcaaagt tcccccctta tattaataaa
    13981 tttaattt
//
LOCUS       DAMJYR010000097        13979 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.100, whole genome shotgun sequence.
ACCESSION   DAMJYR010000097 DAMJYR010000000
VERSION     DAMJYR010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13979)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13979)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13979
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.100"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            141..1721
                     /locus_tag="PC471_002763"
     CDS             141..1721
                     /locus_tag="PC471_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-dependent NAD(P)H-hydrate
                     dehydratase/NAD(P)H-hydrate epimerase"
                     /protein_id="HDF2789875.1"
                     /translation="MLIGTSKLSRNVDTACVEEVGIPMIVLMENAVISAMKNMDIDMY
                     NSYTVVCGVGNNGGDGLGIARHLNTYGKEVNVFLVGNLEKLSECSKINYNVLLNMGIN
                     IININKNDLSEKNYGEENEVDKIIKVEKQNIIKNKNLTIFKDRVSKSDVVVDAIFGTG
                     LKRDITGIFKEVIDIVNDNSKNTYSIDIPSGIDSDNGNVLGTCIRANKTISFEFYKRG
                     FLNYDTSALIGDVVVEKIGVPDFITRKYHDEEFITDKTFVKNNIRRRDKHGFKSDFGK
                     VSIVAGSKGFYGASFIATESAVKSGSGLVTLISSEDVQEKVSIRLTEAMTLNFEEERL
                     NKLLNSSDAIGFGPGMGDNSQTFDKLLKIVENSNCPIVLDADGLNVMKDRCYKFLEWK
                     NRFVITPHLGEMARLTGDSIGYIREHRVDVAKEFAQKYNLVVLLKGYQTVITDGKKTY
                     INPTGNSCMATGGMGDCLLGMITSFIGQGMDILEATVSGAYIHGYIGDKLSKAMYTVN
                     ATDLISNISLTMKELLDI"
     gene            1895..2341
                     /locus_tag="PC471_002764"
     CDS             1895..2341
                     /locus_tag="PC471_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HDF2789876.1"
                     /translation="MKLIWLIVAVLFGIAEMLTPSLTLIWFSVGAVILIFLSSFIESI
                     FLQILIFAVISIAMLVVATKKIVKKDKGYKSNTNLQAMMSKKGIVTEEISPNNTGLVV
                     VEHETWTAISIDGEKIEKGSTVEVLKIEGVKLVVKKVDATVNVTNQ"
     gene            2362..3405
                     /locus_tag="PC471_002765"
     CDS             2362..3405
                     /locus_tag="PC471_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH/Band 7/PHB domain protein"
                     /protein_id="HDF2789877.1"
                     /translation="MGTKIVLSIVLIVVVVAISLTCIRVIKQSKVGIIMRLGKFQKVA
                     ETGVHFLIPFLDKMAYVIDLREIVIDFPPQPVITKDNVTMQIDTVVYYKVTDPVRYVF
                     EIANPIAAIENLTATTLRNIIGELDLDETLTSRDIINVKMRTILDEATDKWGIKVNRV
                     ELKNIMPPQDIQVAMEKQMRAERERREAILQAEGNKSAAILQAEGEKQSAILTAEAKK
                     EAMVRVAEGEKESAILVAEGEAEAIRQTAIAKAQGEAEMIKRTQMATAEGLKLVFSAM
                     KEADIDNNILALKSMEALEKMAEGKSTKLVLPSEAVNFLGTFKGIKEVMSDDNKEVLD
                     IKEVLNDNESLKK"
     gene            4176..5336
                     /locus_tag="PC471_002766"
     CDS             4176..5336
                     /locus_tag="PC471_002766"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HDF2789878.1"
                     /translation="MKKEMLAMILAGGQGSRLGVFTKRIAKPAVSFGGKYRIIDFVLS
                     NCSNSGIDTVGVLTQYRPLILNSHIGMGSHWDLDRINGGVYVLQPFMNEKEGNWYNGT
                     AHAIYQNMDFVDTYNPEYVLILSGDHIYKMDYSKMLKFHKEKGSKATIAVIEVPWDEA
                     SRFGIMNTNEDSSIYEFEEKPSEPKSNLASMGVYIFDWKMLRNYFKEAEKNPEINYDD
                     FGKNLIPKMLEDNVGMYAYPFKGYWRDVGTIQSLWDANMDIIKSPETLDLADPKWKIY
                     TNTMAMPPQYIGKNANVHRSMIADGCRILGEVGNSVLSHGVVVGKGSKVIDSVIMPNV
                     VIGENVTIEKAMIGECATINDNVQIKNVNNEINVVSEYENIEPRCVLIEGGL"
     gene            5338..6468
                     /gene="glgD"
                     /locus_tag="PC471_002767"
     CDS             5338..6468
                     /gene="glgD"
                     /locus_tag="PC471_002767"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase subunit
                     GlgD"
                     /protein_id="HDF2789879.1"
                     /translation="MRNECLGIINLNKKGDPAINKLNYGRPIASTPIAGRYRIIDFAL
                     SNMINSGITKVGIFAKEKYRSLTDHIGSGKDWDLSRKKGGLSIFSPENTKYRNTYSHR
                     EGDIYTILANLDYIEKSEEEYILIAPSYMLCNLNYSQALEYHKKSHNDITIIYKNVNN
                     ANKDFAGNLTLNLDSNNRVINVGNNLGKFPRANICMETYIMKREDFVECIYNIVNKGN
                     YCYLEEFIIEEAENMKIGAYEYTGYLKCVNSVESYFEMSKDLLNLEVADELLYSERKI
                     FTKEKNESPTIYTDSAKVENSFIASGCLIEGTVKDSIIFRKVNVEKGTVIENSIVMQN
                     CVIKSNAKLYNAILDKNTSVSNGKELRGDEKYPLVVEKNTNI"
     gene            6497..7939
                     /gene="glgA"
                     /locus_tag="PC471_002768"
     CDS             6497..7939
                     /gene="glgA"
                     /locus_tag="PC471_002768"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011053260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HDF2789880.1"
                     /translation="MKVFYVTAECWPFAKTGGLGDVSYALPKELKKEGVDVRVIMPKY
                     STIPSYLKDQLKEIAVFSVRVGWRNQYCGLLEMELDGVKFYFIDNEFYFRREDERKSI
                     YGYGDDAERYTFFTDAVLEAISRIDFYPDVIHINDWHTGMLPLILKERYATLEGYKNI
                     KTMYTIHNLQYQGVFDKHVLYDILDLPQKYFDNGDIEYYGSINFMKAGINFADKIITV
                     SPTYANEIQTSFYGEQLDGLLRKESGKLKGILNGIDYDLNDPAKDKDIFVHYDVDSID
                     KKVENKLRLQDILGLKKDSSIPLIGIVSRLVSQKGFDLIAYMMPELMREDLQIVVLGT
                     GEHQYQSMFNYYDSNFSDKVSARITFNASLAQQIYAASDMFLMPSLFEPCGIGQMLAM
                     RYGSLPIVRETGGLRDTVTPYNKFTGEGNGFSFKNYNAHEMFFCLKNAIKVFKDKEKW
                     IKLVENAMKTDNSWKKSAKEYIETYRDICD"
     gene            7953..10373
                     /locus_tag="PC471_002769"
     CDS             7953..10373
                     /locus_tag="PC471_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HDF2789881.1"
                     /translation="MVTISKKKFKQKFEVKMYSLYAQSIKEATDEQLLNVLCSLLKDE
                     IAKKWVATKLDKKKEVYYFSLEFLIGRQLKSNLLNLNIEEEVREGLSELGINLDDLIE
                     AEVDPALGNGGLGRLAACFLDSMASLNISGQGYGIRYKYGLFEQKFVNGYQVEVPDNW
                     LTEGRYAWETVRPNEATMVKFGGEVELIKEGSHLKVIHKNYLPVMAMPYDIPIIGYQN
                     QCINTLRLFKSEIPKRDFGELTSNALNYSGSYEEALKHKYYTEEISQVLYPDDSNYAG
                     KLLRLKQEYFFVSAGIQDIIRKYKKNKLNINNLFDKVAIHINDTHPTLCIPELMRVLL
                     DEENLSWDEAWEITKKTVSYTNHTIMSEAMEKWPVSMMKELLPRIYMIIEEINRRYVE
                     ELNNKGYDQDRIKRMSIIDCDNINMANLCIVTSHSVNGVAKLHTHILETEVLKDFYQD
                     EPNKFNNKTNGIAHRRWLISSNPQLSNLITDLIGDSWKKDTLQLKNIEKFKNDSSVLQ
                     RLDDIKYNNKANLAKFIKDKYDLNVDPSSIFDVQVKRLHAYKRQLLNIFNVLHMYHEL
                     LDNPNLNLDPRTFIFGAKAAPGYYLAKCIIKFINSVASTINNDVRVKDKLKVVFLENY
                     GVSLAEIIIPAANVSEQISTTTKEASGTSNMKFMMNGAITLATLDGANVEICEQVGKE
                     NMFLFGLSAEQVLNYNKYGGYSSLDLYHSNMDIKRVVDDLINGFIPNLGEEGRSIYNS
                     LTTYNDEYFVLRDFENYGQAQADINRLYRDKEKWNKMSLVNIANSGFFSSDRTISEYA
                     KDIWFKRV"
     gene            10378..12243
                     /locus_tag="PC471_002770"
     CDS             10378..12243
                     /locus_tag="PC471_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HDF2789882.1"
                     /translation="MQSVIEYNSWDKNFKAPFGALKFDEELTICVKVNEGYNIKSISL
                     EINREEEMRTITLNEELDNDKLGKCFCGKIEKFDGTGVYFYYFKVDVEMDGQIKTLFY
                     GKNRDNGYSCEYNYSDINKYQITVYKDFKVPTWYKEGILYHIFVDRFNNGNRSGKVDN
                     PKKNSFIYGNWEDTPMYIKDSQGDVIRWDFHGGNLRGIINKLGYLKKLGVSILYLSPI
                     FEASSNHKYDTGDYKKIDPMFGDEDTFKELIDKAKEKGISIVLDGVFSHTGADSKYFN
                     MYGNYNSLGAYQSKESPYYSWYMFEEFPQKYKSWWDVKTLPNINELEHSYMDYIIYDN
                     DSVINKWVNMGIKGWRLDVADELPTKFIRELKKELKKADDDSILIGEVWEDASNKISY
                     GQRRSYLLGEELDSVMGYPFRNNMFSFLKGEINSYELCNRYMQIKENYPKESFRSNLN
                     LIGTHDVTRAKTELNDDVDLVKLAVAIQMTFEGVPYIYYGDEAGLCGGVDPDNRRTYP
                     WKNEDEDMLNFYRDVIRIRNKNKLLSSGNTEFIYTNNDSVFSFIRVNENNDRMLILVN
                     RSENVESISLCIESSFIEEIPIKYSLKNANSTIQIENNELKVDLDSKSFRIFRVN"
     gene            12488..13141
                     /locus_tag="PC471_002771"
     CDS             12488..13141
                     /locus_tag="PC471_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDF2789883.1"
                     /translation="MKVDPLTTQVYDYISKKIQNGEYEANQRITESEICKSIGVSRTP
                     AREALTRLAGENLLEKIPNKGFVVKEFQEKEKLDTYSVIGVLDALAGSSALQNLTESD
                     LVKMEELTEMMAVSIKYKNYNSYLKLSNEFHDIYITKSDNQVLVNLLNSLRYNFMSKS
                     YTSDNEDELYKMLTYSNNQHIEVVKSMRENDLENVERILREHWKTIPMEEMKKEKLL"
     gene            13237..13836
                     /locus_tag="PC471_002772"
     CDS             13237..13836
                     /locus_tag="PC471_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HDF2789884.1"
                     /translation="MYPNKEIAIEELEVAGKMNPGPWIKHSYNVANVAKLIAEQCENL
                     NSEKAFVCGLLHDIGRRTGISSVRHIIDGYDYVISKGWDEVARVCLTHSFPVKDIEAD
                     IGNKDISKEQYDFINHYLNNLEYNDYDKLIILCDALADANGFCILEKRFVDTTRRYGI
                     YPFSVERWNKTYEYKEYFEKIINKSIYTLLPNIEKSIYY"
BASE COUNT         5614 a         1343 c         2567 g         4455 t
ORIGIN      
        1 aaattttttg ttattttaaa ttatttcaaa actcttagtt ttagtgattt tatttatgat
       61 atttatggga ttaaaaagat ttcaatagat atttttggta agtatttaag aagtctttaa
      121 ctttatagga gtgataattt atgctaatag gtacatctaa attaagcaga aatgttgata
      181 ctgcatgtgt agaagaagta gggattccaa tgattgtatt gatggaaaat gcggttatat
      241 ctgcaatgaa aaatatggat atagacatgt ataatagtta tacagttgta tgtggtgttg
      301 gaaataatgg aggagatggt cttggaatag caagacatct aaatacatat ggaaaagaag
      361 taaacgtatt tttagtaggt aatcttgaaa aactaagtga atgctctaaa ataaattaca
      421 atgtactttt aaatatggga attaatatta taaatattaa taaaaatgat ttaagtgaaa
      481 aaaattatgg agaggaaaat gaagtagata aaattattaa agttgagaaa caaaatatta
      541 taaaaaataa aaatttaaca atttttaaag atagagtatc taaaagtgat gttgtagtag
      601 atgctatatt tgggactgga ttaaaacgtg atataactgg tatttttaaa gaagttattg
      661 acatagtaaa cgataatagc aaaaatacgt attctataga tattccttca ggtatagata
      721 gcgataatgg aaatgtatta ggaacttgta ttagagcaaa caaaacgatt agctttgagt
      781 tttataaaag aggttttcta aactatgaca ctagcgcact tataggtgat gtagttgtag
      841 aaaagatagg tgttccagat tttattacaa gaaaatacca tgatgaagag ttcataactg
      901 ataagacttt tgtaaaaaat aatattagaa gaagagataa gcatggtttt aaaagtgatt
      961 ttggaaaagt atctatagtt gcaggttcaa aaggatttta tggtgcaagt ttcatagcaa
     1021 ctgaatctgc tgttaagagt gggagtggac ttgtaacatt aattagtagt gaagatgtgc
     1081 aagaaaaggt atctattaga cttacagaag caatgacttt aaattttgaa gaagaaagat
     1141 taaataaatt acttaattct agtgatgcaa ttggttttgg tccaggtatg ggagataata
     1201 gtcaaacatt tgataaatta cttaagatag tagaaaattc taattgccca atagtattag
     1261 atgcagatgg attgaacgtt atgaaggaca gatgttataa gtttttagaa tggaaaaata
     1321 gatttgtaat tacacctcat ttgggagaaa tggccaggtt gacaggagat tcaattgggt
     1381 atataagaga acatagagta gatgttgcaa aagaatttgc tcaaaaatat aatctggtgg
     1441 tgcttttaaa aggttatcaa actgtcataa cagatggtaa aaaaacatat ataaatccta
     1501 caggaaatag ctgtatggca actggtggaa tgggtgattg tttgcttgga atgattactt
     1561 catttatagg tcaaggtatg gatatattgg aagctacagt tagtggagca tatatacatg
     1621 gctatatagg agataaattg agcaaagcaa tgtatacggt aaatgctacg gatttaataa
     1681 gtaacatttc attaacaatg aaagagttgt tggatatata attggtgata tttgatacta
     1741 ctttgtaaca attttaacaa agtatgttga tataatacta ttaattaatt aatttatttt
     1801 attgttttgt aatttaaatt tatattcaaa aacattgtat aaacatataa tttatatagg
     1861 gttttataat tcatatgaaa agagggtaga tagtatgaag ctaatatggt tgatagtagc
     1921 agtcttattt ggaatagcag aaatgttaac accaagctta acattgatat ggtttagtgt
     1981 aggagctgta atacttatat ttttaagtag ctttatagag agtattttct tacaaatttt
     2041 aatatttgca gtaatttcta tagcaatgtt agtcgtagct actaaaaaaa tagtaaaaaa
     2101 agataaaggc tataaatcta acactaattt acaagcaatg atgtctaaaa aaggtattgt
     2161 tacagaagag atttctccaa ataacacagg tctagttgta gttgaacatg aaacatggac
     2221 ggctatttct atagatggtg aaaagataga aaaaggttct acagtagaag tattaaaaat
     2281 tgaaggggta aaattagttg ttaaaaaagt agacgccaca gttaatgtga caaaccaata
     2341 gaataaagga ggatttatag tatgggtact aaaattgttt taagtattgt attaatagta
     2401 gtagtcgtag caataagttt aacttgtata agagttataa aacaatctaa agtaggtata
     2461 ataatgagac ttggtaagtt tcaaaaagtg gctgaaacag gtgtacattt cttaatacca
     2521 tttttagata aaatggcata tgtaattgac cttagagaaa ttgtaataga ttttccacct
     2581 caaccagtta taactaaaga taatgtaact atgcagattg atacagttgt atattataaa
     2641 gttacagacc cagttagata tgtgtttgag atagctaatc caatagctgc tattgaaaat
     2701 ttaacagcta caacactaag aaatataatt ggtgaacttg atttagatga aacattaaca
     2761 tcaagagata taataaatgt aaaaatgaga actattcttg atgaagcaac agataaatgg
     2821 ggtataaaag taaatagagt agagttaaaa aatataatgc ctcctcagga tattcaagtt
     2881 gcaatggaaa aacaaatgag agcagaaaga gaaagaagag aggcaatact tcaagcagaa
     2941 ggtaataagt cagctgcaat acttcaagct gaaggtgaaa aacaatctgc tatattaaca
     3001 gcagaagcca aaaaagaagc tatggtacgt gtagcagaag gtgaaaaaga atctgctata
     3061 ttagtggcag aaggtgaagc tgaagctata agacagacag ctattgctaa ggcacaaggt
     3121 gaagctgaaa tgataaaaag aactcaaatg gcaacagcag aaggtttaaa attagttttt
     3181 tcagcaatga aagaggctga tatagacaat aatattctag cattaaaatc tatggaagct
     3241 cttgaaaaaa tggctgaagg taagtcaaca aaactggttt taccttcaga agcagttaat
     3301 ttcctaggaa cattcaaagg aataaaggaa gttatgagtg atgataacaa agaagtactt
     3361 gatataaaag aagttttaaa tgataatgaa tcattaaaaa aataaaagag ttttgttatc
     3421 ataggaagga tatggaaatt aaaggtcgaa ttaattaaat atagcacaaa aatgcacgtt
     3481 gaaaagaaga ggacttataa atttttaagt cttcttcttt gtttttatta gaaagatgaa
     3541 attataaaaa taaattaaca gaatttttat acaatttatc ctttactaat gtaaaaagtg
     3601 ttatataata ttatataaaa catatagcaa ataagcatgg ttttagttat tctatttata
     3661 ttattaatat atatttataa aaatataaac attaatacta agaaaactaa taaataattt
     3721 aaaagtttat atccttttgt ttaaattata gacaaagaag tatcaattaa aaaataaagt
     3781 tgattaataa ttacttgatt ttccttggat aattatttaa caaatattag attatatttt
     3841 gctattacga atttaacaaa ttaaaaaata agcatgttca tatgacacaa aggatttata
     3901 aaaaatgttt ttatatttat aaaataaaat ttgtattatt ttttaaccga taattgaata
     3961 ttaataaata ctatagtgct tatggaaaat aatgtatacg tcggtaagtc cattatgggg
     4021 tatacattat atcaattgta tagttgatat tattttattt ttattaaggt attatttaag
     4081 caacatttat acacgacatt tattgactga taaagtttat aaaagtaaaa atgcaattat
     4141 aaatattaat tactagatga ggggagtgtc attaaatgaa aaaagagatg ttagctatga
     4201 ttttggcagg agggcaaggt tcaagattag gtgtttttac aaaaagaatt gccaaaccag
     4261 ctgtatcatt tggaggtaag tatagaatta tagattttgt gttaagcaat tgttcaaact
     4321 ctggtattga tactgtaggt gttttaactc aatatagacc gttgatatta aattcacata
     4381 taggcatggg aagtcattgg gatttggaca gaataaatgg tggtgtctat gttttacaac
     4441 catttatgaa tgaaaaagaa ggaaattggt ataatggtac agcacatgcg atatatcaaa
     4501 atatggactt tgtagatacc tataatccag aatatgtatt gatattatct ggtgaccata
     4561 tatacaagat ggattatagc aaaatgttaa aatttcataa ggaaaaaggc tctaaagcta
     4621 caatagcagt gattgaagtt ccttgggatg aagcttcaag atttgggata atgaatacaa
     4681 atgaagattc aagtatctat gaatttgaag aaaaaccaag tgaacctaag agcaatttgg
     4741 catctatggg agtatacata tttgattgga aaatgttgag aaattatttt aaggaagcag
     4801 aaaaaaatcc agaaataaat tatgatgatt ttggaaaaaa tttaatacct aaaatgttag
     4861 aagataatgt tggaatgtat gcttatccat ttaaaggata ctggagagat gtaggaacta
     4921 tacaaagtct atgggatgct aatatggata taataaaatc tccagaaaca cttgatttag
     4981 cagaccctaa gtggaagata tatacaaata ctatggcaat gcctccacaa tatataggta
     5041 aaaatgcaaa tgttcataga tcaatgatag ctgatggttg tagaatttta ggtgaagtag
     5101 gtaactctgt attatcacat ggagttgttg ttggtaaagg aagtaaagta attgattcag
     5161 taataatgcc aaatgttgta ataggtgaaa atgtaactat agaaaaagct atgataggtg
     5221 agtgtgcaac tataaatgat aatgttcaga taaagaatgt aaataatgaa ataaatgtag
     5281 tttctgagta tgaaaatatc gaacctagat gcgtattaat agaaggaggt ctttaagatg
     5341 agaaatgaat gtttagggat aataaattta aacaaaaaag gagacccagc aataaacaaa
     5401 cttaactatg gaagaccaat agcatctaca ccaatagctg gaagatatag aataatagac
     5461 tttgctttat ctaatatgat aaattcagga atcactaaag ttggaatatt cgcaaaagaa
     5521 aaatacagat cattaacaga ccatataggt agtggtaaag attgggattt aagtagaaaa
     5581 aaaggtggcc tatcaatttt tagtccagaa aacactaagt atagaaatac ttactcacat
     5641 agagaaggtg atatatatac tatcttagct aatctagatt atatagaaaa gagtgaagaa
     5701 gaatatattt taatagctcc aagctatatg ctatgcaatt taaattattc tcaagcattg
     5761 gaataccata aaaaatctca taatgatata actataatat ataaaaatgt aaataatgca
     5821 aataaagatt ttgctggtaa cttaactcta aatttagata gtaacaatag ggtaataaat
     5881 gtaggaaata atttaggtaa gttccctaga gcaaacatat gtatggagac atatattatg
     5941 aagcgtgaag attttgttga atgtatatat aacattgtaa ataaaggcaa ttattgttat
     6001 ttagaagaat ttattattga agaagcagaa aacatgaaaa taggagccta tgaatataca
     6061 ggatatctaa aatgtgtaaa ttctgttgaa tcatattttg aaatgagcaa agatttatta
     6121 aatttagaag ttgcagatga attattatat tctgaaagaa agatatttac aaaagaaaag
     6181 aatgaatcac ctacaattta tacagatagt gctaaagtag aaaatagctt tatagcatct
     6241 ggttgtttaa tagaaggtac tgtaaaagat agtatcatat ttagaaaggt aaatgttgaa
     6301 aaaggaacag ttatagagaa ctcaatagta atgcaaaatt gtgtaataaa gtcaaatgca
     6361 aaactatata atgcaatatt agataaaaat acaagtgtat caaatgggaa agaattaaga
     6421 ggagatgaaa aatatcctct tgtagtggaa aagaatacaa atatataaat aaatttttta
     6481 gaagggggaa tgactaatga aggtttttta tgtaacagca gagtgttggc cctttgcaaa
     6541 aactggagga ttaggagatg tttcttatgc attgcctaaa gaattaaaaa aagaaggtgt
     6601 agatgtaagg gtaataatgc ccaaatattc aactatacca agttatttaa aagatcaact
     6661 aaaagaaata gctgtgttta gtgtacgtgt tggatggaga aatcaatact gtggtctttt
     6721 agaaatggaa ttggatggtg taaaatttta ctttatagat aatgaatttt attttagaag
     6781 agaagatgaa agaaagagta tctatggata tggagatgat gcagaaagat atactttctt
     6841 tacagatgca gttttagaag ctataagtag aattgatttt tatccagatg taatacatat
     6901 aaatgattgg catacaggga tgttaccatt aattctaaaa gaaagatatg caacccttga
     6961 ggggtataaa aatataaaaa ctatgtatac tatacataat ctccaatatc aaggtgtatt
     7021 tgataagcat gttttatatg atatattgga cttaccacaa aaatactttg ataatggaga
     7081 tatagagtat tatggctcaa taaattttat gaaagctggt ataaattttg cagataaaat
     7141 tataactgtt agtcctactt atgcaaatga aatacaaaca tctttttatg gcgaacaatt
     7201 agatggatta cttagaaaag agtcaggaaa attaaaaggt atattaaatg gtatagatta
     7261 tgatttaaat gaccctgcaa aagataaaga tatatttgtt cattatgatg tagactcaat
     7321 tgacaaaaaa gtagaaaata aattgagatt acaagatata ttgggactaa aaaaagattc
     7381 atctattccg ttgataggta tagtttcgag attagtttct caaaaaggat ttgatttaat
     7441 agcatacatg atgccagaac ttatgagaga agatttacag attgtcgttt taggaactgg
     7501 agaacatcaa tatcaatcaa tgttcaacta ttacgattct aatttttcag ataaagtatc
     7561 agctagaatt acttttaatg catcattggc tcaacagata tatgctgcaa gtgatatgtt
     7621 cttaatgcca tcattatttg agccttgtgg tataggtcaa atgttagcta tgagatatgg
     7681 ttctttacca atagttagag agactggagg gcttagagat acagtaacac cttataataa
     7741 atttactgga gaaggcaatg gatttagttt taaaaattac aatgctcatg agatgttttt
     7801 ctgtttgaaa aatgcaatca aagtatttaa ggacaaagaa aaatggatta aattagttga
     7861 aaatgcaatg aaaacagata atagttggaa aaaatcagct aaggagtata tagaaacata
     7921 tagagacata tgtgattaaa ggagtggttt ttttggtaac aataagcaaa aagaaattca
     7981 agcaaaaatt tgaagtaaaa atgtatagct tatatgcaca atctatcaag gaagctactg
     8041 atgaacaatt gttaaatgta ttatgtagtc tattgaaaga tgagatagca aaaaaatggg
     8101 ttgctacaaa gttagataag aaaaaagaag tttattattt tagtttagaa tttttaatag
     8161 ggagacagtt aaaatctaat ttattaaatt taaatattga agaagaagtt agagaaggtt
     8221 tatctgaatt aggaatcaac ttggatgatt taattgaagc tgaagtagac ccagccttag
     8281 gaaatggtgg acttggtaga ttagcagctt gctttttaga ttctatggca tctttgaaca
     8341 taagtggtca aggatatgga ataaggtata aatatggtct gtttgaacaa aaatttgtaa
     8401 atggttatca ggtagaagtg cctgataact ggcttacaga aggacgatat gcatgggaaa
     8461 ctgtaagacc aaatgaagca acaatggtga agtttggtgg tgaggtagaa ttaatcaagg
     8521 aaggtagtca tttaaaagta attcataaaa actaccttcc tgtaatggca atgccttatg
     8581 atataccaat aatcggatac caaaaccaat gtataaatac tttaagattg tttaagagtg
     8641 aaataccaaa gagagatttt ggtgaactta cctcaaatgc tctaaattat tctggtagct
     8701 atgaagaggc tttaaaacat aagtattata ctgaggaaat atcacaggta ttatatcctg
     8761 atgattcaaa ctatgcaggt aagttgttga ggttgaaaca agaatacttt tttgtaagtg
     8821 cagggataca agatataata agaaaatata aaaagaacaa attaaatatt aataatttat
     8881 tcgataaggt agcaattcat ataaatgata cacatcctac cttatgcata ccagaactta
     8941 tgagagtttt gcttgatgaa gaaaatttat cttgggatga agcatgggaa ataactaaaa
     9001 aaacagtgtc ctatactaat catactataa tgtcagaagc aatggaaaaa tggcctgtaa
     9061 gtatgatgaa agaactatta ccaagaattt atatgataat agaagaaata aatagaagat
     9121 atgtagagga attaaataat aaaggatatg accaagatag aataaaaaga atgagtataa
     9181 tagactgtga caatatcaat atggcaaact tatgtattgt cacaagtcat agtgtaaatg
     9241 gagttgctaa gcttcatact catattcttg aaacagaggt attaaaagat ttctatcaag
     9301 atgaaccaaa taaatttaat aataagacta atggaattgc acataggcga tggcttataa
     9361 gttcaaatcc tcaacttagc aatttaatta cagatttaat tggtgattca tggaagaaag
     9421 atacattaca attaaaaaat attgaaaaat ttaaaaatga ttcctctgtt ttacaaagac
     9481 ttgatgatat aaaatacaat aataaagcaa atctagcaaa gtttataaaa gataaatacg
     9541 atttaaatgt agacccaagt tcgatatttg atgttcaagt taagagattg catgcgtata
     9601 aaagacagtt actgaatata tttaatgtac ttcatatgta tcatgaatta cttgataatc
     9661 ctaatttgaa tcttgaccca agaactttta tatttggcgc aaaagcagca ccaggatatt
     9721 atttggctaa atgtataatt aaatttataa attctgttgc aagtactata aataatgatg
     9781 ttagagtaaa ggacaagtta aaagtagtat ttttagagaa ttatggagta tcattagcag
     9841 aaataataat accagcagca aatgttagtg agcaaatttc aactacaact aaggaagcat
     9901 ctggaacaag taatatgaag tttatgatga atggtgctat aactttggct acacttgacg
     9961 gtgcaaatgt agagatatgt gaacaggttg gtaaagaaaa tatgttttta tttggactta
    10021 gtgcagaaca agtactaaat tataataaat atggtggata ttcttcactt gacctatatc
    10081 attctaatat ggatataaaa agggtagtag atgatttaat aaatggattt attcctaatc
    10141 ttggcgaaga aggaagaagt atttataatt cactcactac ttacaatgat gagtattttg
    10201 tattgagaga ttttgaaaat tatggtcaag ctcaagcaga tataaacagg ttatatagag
    10261 ataaagaaaa atggaataaa atgtctttag ttaatatagc taattctgga tttttttctt
    10321 ctgatagaac aatttctgag tatgcaaaag acatttggtt taaaagggtg tgattaaatg
    10381 caaagtgtaa tagaatataa ctcttgggat aaaaatttta aggctccgtt tggagcttta
    10441 aaatttgatg aggaattgac catttgtgtt aaagtaaatg agggatacaa tatcaagtct
    10501 atttccctag aaataaatag agaagaagaa atgagaacaa taactttaaa tgaagaatta
    10561 gataatgata aacttggaaa gtgcttttgt ggtaagattg aaaagtttga tggtacaggt
    10621 gtttattttt attactttaa agttgatgtt gaaatggatg gtcagataaa gactttattt
    10681 tatggaaaaa acagagataa tggatattct tgtgaatata attatagtga tattaataaa
    10741 tatcaaatca ctgtttacaa agattttaaa gtaccaacat ggtacaaaga aggaatactt
    10801 tatcatatat ttgtagatag attcaataat ggaaatcgta gtggtaaggt tgataatcct
    10861 aaaaaaaata gtttcatata tggaaattgg gaagatacac ctatgtacat aaaggatagt
    10921 caaggggatg ttataagatg ggatttccat ggtggaaatt taagagggat aatcaataaa
    10981 cttggatatt taaaaaaatt aggtgtaagt atactatatc taagccctat atttgaagcc
    11041 tctagtaatc ataagtatga tacaggtgac tataaaaaaa ttgacccaat gtttggggat
    11101 gaagatacat ttaaagaact tattgataaa gcaaaagaaa aaggtatatc aatagtatta
    11161 gatggagttt ttagtcatac tggagcagat agtaaatatt ttaatatgta tggaaactat
    11221 aatagtttag gggcatatca atctaaagaa tctccatatt attcatggta tatgtttgaa
    11281 gaatttcctc aaaaatataa aagttggtgg gatgttaaga cattgccaaa tataaatgaa
    11341 ttagaacata gctatatgga ttatataata tatgacaatg atagcgttat aaataaatgg
    11401 gtaaatatgg gtattaaggg ctggagacta gatgttgcag acgaattacc aactaagttt
    11461 ataagagagt taaaaaagga attaaaaaag gcagatgatg actcaattct tataggtgaa
    11521 gtatgggaag atgcatcaaa taagattagc tatggtcaga gaagaagtta tttattaggt
    11581 gaagaattag attctgttat gggttatcct tttagaaata acatgttttc tttcttaaaa
    11641 ggagaaatta attcatatga actttgtaat agatatatgc agattaaaga aaattatcca
    11701 aaagaatcat ttaggtctaa tttaaattta attggaacac atgatgttac aagagctaaa
    11761 acggaattaa atgatgatgt agatttagtg aagcttgctg tagctataca gatgacattt
    11821 gaaggagtac catatatata ttatggagat gaagcaggtc tttgtggagg tgttgaccca
    11881 gataatagaa gaacttatcc atggaaaaat gaagacgaag atatgcttaa tttttataga
    11941 gatgttataa gaattagaaa taagaataaa cttttaagtt ctggaaatac agaatttata
    12001 tatactaata atgattctgt attttctttt attagagtta atgaaaataa tgatagaatg
    12061 ttaattttag taaatagaag tgaaaatgtg gaaagtatat cattatgtat agagagtagt
    12121 tttattgagg aaatacctat aaagtatagt cttaaaaatg caaatagtac tatacaaatt
    12181 gaaaataatg agttgaaagt agatttagac tctaaatcat ttagaatttt tagagtaaat
    12241 taaatatctt tttttagaaa aataaataga aaataaggtg aatgtggaaa gagttttgtt
    12301 ttatcaaaac tttttctact ttttttgtat aaattatcat aagaatttta gtatttcaaa
    12361 atattacaaa gaagtataaa aaatatcata attgtgatat tatataaatt agtaagataa
    12421 aactgaactc tctaccaaag ttgtagggag atttaacttt tataatctta atatggagga
    12481 acgtcttatg aaggtagatc cacttacaac tcaagtatat gactatatat caaagaaaat
    12541 ccaaaatgga gaatatgaag ctaatcagag aattactgaa tctgagattt gtaaatctat
    12601 cggagttagt agaacaccag cgagagaagc attaactagg ttagcaggtg aaaatttact
    12661 agaaaaaata cctaataagg gatttgtagt aaaagaattt caggaaaagg agaaattaga
    12721 cacctattcc gtaattggag tattggatgc gttagccggt tcttcagcat tacaaaattt
    12781 aactgaatca gacctagtaa aaatggaaga attaacagaa atgatggcgg tctctataaa
    12841 gtataaaaat tacaatagct atttaaaatt aagtaatgaa tttcacgata tatacataac
    12901 taaatcagat aatcaggtgt tagtaaatct attgaactct ttaagatata attttatgtc
    12961 aaagtcatat actagtgata atgaagatga gttatataaa atgttgacat acagcaataa
    13021 tcaacatatt gaagtagtaa aatctatgag ggagaatgat ttagaaaatg ttgaaagaat
    13081 tttgagagaa cattggaaaa cgatacctat ggaagagatg aaaaaagaga agcttttata
    13141 aagtttctct ttatttttta taccatttta tcactatttt tttgaattat taagtagaaa
    13201 ataataatta atttaaactt gaggaggaaa actaatatgt atccaaataa agaaattgca
    13261 atagaagagt tagaagtggc tggcaaaatg aatcccggtc catggataaa acattcttac
    13321 aatgttgcta atgtagctaa attgattgca gagcaatgtg aaaatttgaa tagtgaaaag
    13381 gcctttgtat gtggattatt acatgacatt ggaagaagaa ctggtatttc ttctgtaagg
    13441 catattattg atggatatga ttatgttatt tctaagggat gggatgaagt ggcgagggta
    13501 tgtttgacac attcttttcc tgttaaggat atagaagctg atattggaaa taaagatatt
    13561 agtaaagaac agtatgattt tatcaatcac tatttaaaca accttgaata taacgattat
    13621 gataagttga ttattctttg tgatgcatta gcagatgcaa atgggttttg tattttagaa
    13681 aaaagatttg ttgatacaac acgaagatat ggaatatatc cattttcagt tgaacgatgg
    13741 aataagactt atgagtataa agaatatttc gagaaaataa tcaacaagtc tatatataca
    13801 ttgttaccca atattgaaaa atctatatat tattaaatcc taagttaata atttaatatc
    13861 cagtatcacc agcagttggt cattatgatt gactgctttt attatgctta tattaaaaaa
    13921 atacactatg gtttgtgtat atcaaagtta tatggaatga aaagagaatt tattttatc
//
LOCUS       DAMJYR010000098        13960 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.101, whole genome shotgun sequence.
ACCESSION   DAMJYR010000098 DAMJYR010000000
VERSION     DAMJYR010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13960)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13960)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13960
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.101"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(166..897)
                     /locus_tag="PC471_002773"
     CDS             complement(166..897)
                     /locus_tag="PC471_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2789885.1"
                     /translation="MIRKKVYILISIAICDDESYMLNHLKENIELYMKQKNLLYNIEL
                     FNKVEDLLCNHEKFNIVFLDIQMEEINGMQAARKLRSYGNECFIIFVTVLNEFVFDAF
                     EVDAINYLIKPLSNEKLYKTLDKIVTKLDDNESNYITIQKGYSYWKLKLADILYCEVI
                     KRKIYVHLKKETVDYYETIENLERSLPTNFFRCHRSYIINLQYVYGYENGNAVMESGD
                     RIPVSRLRQQIFSKVMLEFMKNRRI"
     gene            complement(1174..2481)
                     /gene="grdB"
                     /locus_tag="PC471_002774"
     CDS             complement(1174..2481)
                     /gene="grdB"
                     /locus_tag="PC471_002774"
                     /EC_number="1.21.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012158817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(1432..1434),aa:Sec)
                     /transl_table=11
                     /product="glycine reductase complex selenoprotein B"
                     /protein_id="HDF2789886.1"
                     /translation="MSKIRVVHYINNFFAGIGGEEKADIPPEKRAGVVGPGIAFQSQF
                     NDDAEIISTVICGDTYFGENIESATQELLKMIKEESPDLFIAGPAFNAGRYGVACGTI
                     CKAVEEELNIPVITGMYKENPGVDMFKLDLHIVSTGNSAASLRKVVPIMTKLGLKLVR
                     GEEIGPPEEEGYIMRGIRKNFFHELRGSERAIDMLVKKMKGESFETEYPMPEFDRVTP
                     MKPVKDLSKIKLALVTSGGIVPIDNPDKIESSSATKYGIYDLTDMDSMSNKEFTTIHG
                     GYDRAYVLENPNLVVPLDVVRELEKEGVIGELVDYFITTTGTGTSVGNSKRFGEEFSK
                     KLLEDDVDAVILTSTUGTCTRCGATMVKEIERAGIAVVHICTVVPISLTIGANRIVPA
                     VGIPYPLGNPMLGEEESKKIRKNIVLKALNALTEDIEEQTVFE"
     gene            complement(2567..3853)
                     /locus_tag="PC471_002775"
     CDS             complement(2567..3853)
                     /locus_tag="PC471_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006807454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase component B
                     subunit"
                     /protein_id="HDF2789887.1"
                     /translation="MKLELGNIFIKNVEFGEKTEVKDGVLYISSEEIEKIVLEDERIV
                     SVNVELARPGESIRIAPVKDVIEPRVKIGDESKIFPGIINKVKTVGSGRTHVLLGACV
                     VTCGNIVGFQEGVIDMSGPTAKYTPFSKTNNICIVIKAKDGIDTHDYEEAARIAGLKI
                     GAYVGEAGREVEPDEVIVYETKPLLKQVKEYPDLPTVAYVHMLQSQGLLHDTYYYGVD
                     AKQIVPTFMYPTEIMDGAIISGNCVAPCDKVTTFHHLNNPVIHDLYKRHGKDLNFIGV
                     ILTNENVFLVDKERSSDMVAKLIEFLGVDGVLVTEEGYGNPDTDLMMNCRKCSEVGAN
                     VVLITDEFPGKDGKSQSIADATKEADAVVSCGQGNLVVHFPAMEKIIGTLDYVEMMIG
                     GYKGCLNEDGSMDAELQIIIASTIANGYNHLTARYY"
     gene            complement(4132..5454)
                     /locus_tag="PC471_002776"
     CDS             complement(4132..5454)
                     /locus_tag="PC471_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HDF2789888.1"
                     /translation="MKILLVDDELEYGVVMKKILQKKGYLVDIALSGEEAINIIKKDK
                     NYDLVLSDVMMKNMDGVQLLDRIKSINRDIEVILVTGYGSIENAVDAMKRGALSYFIK
                     SNPIENLLEEVEKVKASKISVSVQKNNFEFTLESKNRDFNNIIKIAKKAACKDVNILI
                     LGESGVGKDILARYIHSISPRKNEIFVPVNCCSFSENLLESELFGHEKGSFTGAVDSR
                     KGRFELSNKGTLFLDEIGDIPLNVQVKLLRTLEDKSIERIGSNKSIKVDFRLICAMNK
                     EPKVEISNGNIREDFFYRISTITITIPPLRKRREDLTTLIEFFLNKYQIEHDKKIHSI
                     DKEVKDFLLNYNYPGNIRELKNIINRLVVLSEEGNLSKDNLNLISNNVYIDDKISIRP
                     LREIRKEFECEYIEKVLSLCGNNISNTAKKLEISRRQLTNKISEYNIK"
     gene            complement(5454..7448)
                     /locus_tag="PC471_002777"
     CDS             complement(5454..7448)
                     /locus_tag="PC471_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HDF2789889.1"
                     /translation="MNKKKIVIIGIIYSFLVVFSLTNMYVNMEYNLNVFEYIKKSLPF
                     TEEEKKWLEKHKNLIYSSDQSSPPLRYKGKEDGQYKGIIVDLINSLSIQIGRDFYFKP
                     NNWWKESFVNSIDDSIKFFDLIPSKERANKFIFTDPIYTLSANILKDKKSQDINSYMD
                     LKGKTVAIPEGDYSINFLKQKIQDINILLTPDIKTGVNHLMSGKVDAVVGDEPVLRYY
                     INNYGLSNKYSVLSNPIYTKKAVLAVPKQYEELVSILNKGIFKLQKNGVYKDLKKKWY
                     STYNEVDDILYERGIVPSIYLFIGIILISIYVFYSYTYLLKIEIKRKTEQVIENKKTL
                     EATFNSITDIIMLVDENNNIVESNKVLYDFMGEMSYKIADLISMIKGVIENTFSENTN
                     KTSEIEIHNKILKINTFPVEYKKNNTEYIVVLIKDITNDKIVEAKLLRENKMISIGQL
                     ASGVAHEIRNPLGIIRNNCYLLKDNVTMEEVNDCVKSIESNVDRASNIITNLLNFARI
                     SDDNLEHINIKNFIENIVKLQYKMLQLKNVEIKIDCEHNLICYINGESLKHVFINLIS
                     NSIDAIHQDGKIIIYCYEKNHCLFIDFKDNGEGIKEDVLKDIFNPFYTTKPIGEGTGL
                     GLYITYNEIKKNNGDISVESKLGVGTCFHIKIPLNKEVTI"
     gene            complement(7535..8782)
                     /locus_tag="PC471_002778"
     CDS             complement(7535..8782)
                     /locus_tag="PC471_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HDF2789890.1"
                     /translation="MQITTFGAIFGLLIAIILIIKKFQAVYSLMLGAFIGGLVGGANI
                     TQTVDFMANGAMNISPSILRALASGVLAGSLIKTGAVDKISEQIVKIFGEKRALFSIA
                     ISTMVLAGVGVNLDVSIITVAPIGLYIGRKLNYSKLSILLAMLGGGKAGNIISPNPNT
                     IAVADNFSVNLSSVMMANIIPAIIGVVITVILASILINKGNKVQSYEILEQREDLPSL
                     FKSLCGPIIAIFLLFLGNVSPIVIDPMIALPIGGIVTLIVTGNLNNSREYLTFGLSKM
                     QGVCILLLGTGTIAGIIQMSELQQSTIGALQFLNMPQFLLAPVSGILMSLATASSTAG
                     ATIASSTFHDAIINGGLSPISGASIVNAGSSVFEQLPHGSLFHTSAGSINMDIGERFK
                     LIPYEALIGIVMTIISTSIQLVL"
     gene            9010..9555
                     /locus_tag="PC471_002779"
     CDS             9010..9555
                     /locus_tag="PC471_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF2789891.1"
                     /translation="MNDLNLGEKIAEVRKKQNLSIRDLAKLADVTPSLLSQIERGLAN
                     PSVNSLKSIASSLNVPLFTFFVSEVDKKNLIVRHDNRKKVILPGSKEVIYEILIPDSS
                     GNLEFAIMDLAPNTSSCVDRITHNGDEIAYVLEGEVKLFMDDDEFTLSKGDSVKVPLG
                     TKHKWQNDSNSESKVIFAVIL"
     gene            9569..10255
                     /locus_tag="PC471_002780"
     CDS             9569..10255
                     /locus_tag="PC471_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HDF2789892.1"
                     /translation="MKTYKFIFFDADDTLFDFKKSESHAFKKLLSEFDLEFNFENYIE
                     SYRNISDKLWLDLEKNIITLNELKLLRFELFANKISLDVDSETLSKMYLNFLGECTFL
                     IPGAIDILQYLKKKYTIVIITNGISAVQNKRLENSKIKGYIDGMVVSEELKISKPNPE
                     IFKYALKKFNCHDKSSALIIGDSLTSDVLGGINSGIDTCWLNSNNSINYTNHIPTYEI
                     NTLIELKKLL"
     gene            complement(10379..10999)
                     /locus_tag="PC471_002781"
     CDS             complement(10379..10999)
                     /locus_tag="PC471_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2789893.1"
                     /translation="MNYNSNKNKYLLKDINLDDIFIDKNIVLKFLGYGNRKAPDTISK
                     IVEREIRNINDILDIKIYISEVDINKTPKECKKAFAVLYTIGDKIDIKMNDYMENCNM
                     MAGLALDKIGIVCLDYINEKIKMYLKGKYDSLKISHEIYPGDKDFEVERQKDIYNYVK
                     NKYNSIEIEINDYHQLSPIKSVAMLILMGDEENLESRCSKCPRKCF"
     gene            complement(10989..12011)
                     /locus_tag="PC471_002782"
     CDS             complement(10989..12011)
                     /locus_tag="PC471_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HDF2789894.1"
                     /translation="MFSDMTLLQYINSEDRGFFLPDMGTNGLFLINKKAYEVYDSVDL
                     QLELAKTMDKYFESDFIYSFCDGITFCETLGLETLRPDYDFPSVLTHTITDRDKLRKL
                     DVPDPYTSGRMPLNIESLSVISKSIKKPLYESIQGPFTLAGQLAGATQLLRCIITDKK
                     FVEELLEFTTELVRRYAVAANKAGAKYISIAEPTSVTLSKDRFDEFIVKNLNKIYNEL
                     DCWKGMHICGDTRELLDNMLSCNIDAVSLDQILDYEEIAPMIPRDIVLIGNLDPIKLL
                     GRSKPDKIRRETLKLLKKMRRYDNFLCDFGCNCLNTTPVENLQAAIKAGRISYKELDR
                     IDIDEL"
     gene            complement(12012..12650)
                     /locus_tag="PC471_002783"
     CDS             complement(12012..12650)
                     /locus_tag="PC471_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423639.1"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HDF2789895.1"
                     /translation="MDILDDIAECILNMGVDNIENLVKIAIDKNIDVEDIYEYGLNKG
                     MIGALDKFENKEYYLSEVIVCTDALNKGINLLKGTGKVKKKSKGVILMSVVEGDTHEI
                     GKNIVKVMVEATGYKVIDLGVNRKSEDIIEEAIKNNVDIIGLSSMMTTTMENMKSVID
                     ELNMIEIDKRPKVIIGGGPVSMEFAEEIGADGYSSNAPKAVKLINKLIGGEV"
     gene            complement(12768..13919)
                     /locus_tag="PC471_002784"
     CDS             complement(12768..13919)
                     /locus_tag="PC471_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004452985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDF2789896.1"
                     /translation="MELRPRSAKLEEFDKVIELINYVFRISRNHKPTMMEEFPLLLSK
                     NNIENMIIISEDDKVISDVNYLIQDVSIQGNRLKVAAIGGVCTHPDYEKRGYSSKILD
                     KVEEKMFYDGVDIVIISGTRSLYSRRNCSLVKSFYKYTIKPEDVKIAYEIVEFDETNF
                     EKDNDLDKMIELYNQNSTRFIRTRDEFQKLLHAATIAWGPIGYKKVFIKENNNIIGYL
                     IIRTIKKEDSTVGEVAEIGLNSVNVENILKYVANKFGLEYLNYKVHVKNLKDQLKCNG
                     TKSLDYQQGTMKIINFTKLCDSLRSYFSQYVDFELLKYMEFKQVENKYIIKYKEEELV
                     IENLDKLNKLFFEKNEEQYNEFKHLKNIYEFATKAFPVDFPWTANLNYQ"
BASE COUNT         4548 a         2428 c         1395 g         5589 t
ORIGIN      
        1 attgaaatta tcgaatataa aataaaacac caaaattttg aattaaaatt tgtaaaattg
       61 attagaaaat agtattgcac tattaccatt aaaattctat atacaataaa agagaagaaa
      121 aaactgtact ttaaaaataa atcggaagaa aataaattaa tcatcttata tcctcctatt
      181 tttcatgaac tcaagcataa cttttgaaaa tatttgttga cgtagtctag aaacaggtat
      241 tctatctcca ctttccataa ctgcattacc attttcatat ccatatacat attgtaaatt
      301 gattatataa ctacgatgac acctaaaaaa atttgttggt aaacttctct ctaaattttc
      361 aatagtctca taataatcta cagtctcttt ctttaaatga acataaatct ttctttttat
      421 aacttcacaa tataaaatat cagcaagttt taatttccaa tatgaataac ctttttgtat
      481 tgtgatataa ttactttcat tatcatcaag ttttgtaact attttatcca aagttttata
      541 tagtttctca ttagatagag gttttatcag ataatttata gcatctactt caaaagcatc
      601 aaatacaaat tcatttaaaa ctgtaacgaa aattataaag cactcattac catatgacct
      661 taattttcta gctgcctgca ttccgttaat ctcttccatc tgtatatcta aaaatacaat
      721 attaaacttt tcatgattgc atagtaaatc ttctacttta ttaaaaagct ctatattata
      781 taataaattc ttctgcttca tgtataattc tatattttcc tttaaatgat ttagcatata
      841 ggattcatca tcacaaatgg caattgaaat caatatatac accttctttc ttatcattat
      901 tctaattatt atataaagtt aattttttct gttcaattaa tatgatctaa tatgttgaat
      961 atttgttcca aaatgtaata atctcgcttt tcatagtata tctaatttga atttgtgtat
     1021 caatattata ataaactaat ttatcacttt tataaattta atttaaaaaa atgcaaattt
     1081 cgaatttacc taataaaaaa agatatcgta aaataatttt aaaaaattat tttttgatat
     1141 ctttttttat ttcagtgttg tgttgtattt ttattattca aatactgttt gttcttctat
     1201 atcttctgtt agtgcattta aagcttttaa aactatattc tttctaattt ttttactttc
     1261 ctcttctcct aacataggat ttccaagtgg atatggtatc cctactgctg gaacaattct
     1321 attagctcct attgtaagtg atattggtac tactgtacat atatgaacaa ctgctatacc
     1381 agctctctca atctctttta ccatcgttgc tccacaacga gtacaagtac ctcatgtaga
     1441 agttaaaatt acagcatcta catcatcttc taacaatttc ttagaaaact cttctccaaa
     1501 tctcttagaa ttgccaacac tcgttcctgt tcctgtagta gttataaaat aatctactaa
     1561 ttctccaatt acaccttctt tttcaagctc tctaactaca tctagtggta caactaaatt
     1621 tggattttca aggacatatg ctctgtcata ccccccatgt atagttgtaa attctttatt
     1681 tgacatagaa tccatatctg ttaaatcgta aattccatat ttagtagcac ttgaagattc
     1741 aattttgtct ggattatcaa ttggaactat ccctccagaa gttactaggg caagttttat
     1801 cttagataaa tctttaacag gtttcatagg tgtaactcta tcaaattcag gcattggata
     1861 ctcagtttca aagctttccc ctttcatctt tttaaccaac atatcaattg ctctttcaga
     1921 ccctctaagt tcatggaaga aattttttct tattcctctc attatatatc cttcctcttc
     1981 aggaggtcct atttcctcac ctctaaccaa tttaagacct agtttcgtca ttattggaac
     2041 tacctttcta agacttgctg ctgaatttcc tgtagacact atatgcaaat ctaatttaaa
     2101 catatctaca cctggatttt ctttatacat gcctgtaatt acaggaatat ttaactcttc
     2161 ttcaactgcc ttacaaatag tcccacaagc tactccatat ctacctgcat taaatgctgg
     2221 gcctgcaata aataaatcag ggctttcttc ttttatcatt tttaaaagtt cttgagttgc
     2281 actctcaata ttttcaccaa aatacgtatc tccgcaaatt acagttgaga ttatctctgc
     2341 atcatcatta aattgactct gaaatgctat tccaggtcct actacaccag cccttttttc
     2401 tggcggaata tctgcttttt cctctccacc tataccagca aagaaattgt ttatataatg
     2461 aaccactctg attttactca tctcttaatc ctccaaatca ctttatagtt attacatcag
     2521 ttagaccaat gattacatca acagaacttt atattcaaat acttatttaa taatatcttg
     2581 ctgtcaaatg attatagcca tttgctatag ttgatgctat tataatttgt agctctgcat
     2641 ccatgcttcc gtcttcattt aagcatcctt tatacccacc tatcatcatt tcaacataat
     2701 ctaaagttcc tataattttt tccattgctg ggaaatgaac cactagattt ccttgaccac
     2761 aagaaacaac tgcatctgct tccttagtag cgtctgctat agattgactc ttgccatctt
     2821 ttccaggaaa ttcatccgtt atcaaaacta catttgctcc aacttcacta cattttctac
     2881 aattcatcat taggtcagta tctggattcc cataaccttc ttctgtcact agaactccat
     2941 caactcctaa aaattctatt aatttagcta ccatatcaga gcttctctct ttatcgacta
     3001 agaatacgtt ctcattagtc aaaataactc ctataaagtt taaatctttt ccatgtcttt
     3061 tatataagtc atgtataact ggattattta aatgatggaa tgttgtaacc ttatcacatg
     3121 gtgctacaca gtttccagat attatagctc catccattat ttcagttgga tacataaatg
     3181 taggcactat ctgtttagca tccaccccat agtaataagt gtcatgtaaa agtccttgtg
     3241 attgtaacat gtgaacatat gctactgttg gcaggtctgg atattctttt acttgtttta
     3301 ataatggctt tgtctcatat acaatgactt catctggctc aacttctctt cctgcctcac
     3361 ctacatatgc acctattttt aatcctgcaa ttctagctgc ttcttcataa tcatgagtat
     3421 ctataccatc ctttgctttt ataacaatac atatattatt tgtttttgaa aaaggtgtat
     3481 atttagcagt aggaccactc atatcaatta cgccttcttg aaatccaact atatttccac
     3541 aagttacaac acaagctcct aaaagtacat gggttcttcc acttcctaca gtcttcactt
     3601 tatttataat tccaggaaat attttgcttt catctccaat tttaactcta ggctctatta
     3661 catctttaac tggagctatt cttattgatt cgccaggtct tgcaagttca acatttacac
     3721 ttacaatcct ttcatcttct aatactatct tttctatttc ttcgctacta atatatagta
     3781 ctccatcttt tacttctgtc ttttctccaa attcaacatt ttttataaat atattcccta
     3841 gctctagctt catatgaaaa tcccccttaa tatatttaat cgcaattaaa agtatttatt
     3901 acgtttatta atatataaag cattttctat gccaattatt taaaatatat attctatgta
     3961 ttaatgtcat agaaattttc atttttaatt tacaccaata ttgaatttag caataattat
     4021 tgtaaatatt aatttttaaa attataataa aaaaagataa acagattttt tctgtttatc
     4081 ttttaataca gtttgagaaa tttttttctc acttaatgag aagttttttt ctcacttaat
     4141 attatactct gatattttat tggttaactg tcttctacta atctccaatt ttttagctgt
     4201 atttgaaata ttatttccac acaaagataa aactttttca atatactcac attcaaactc
     4261 ttttcttatt tctcttaaag gtctaatact tattttatca tctatgtata cattatttga
     4321 tattaaattt aaattgtctt ttgataaatt accttcttca cttaaaacta caagtctatt
     4381 tattatattt tttaattctc ttatatttcc tggataattg taatttaata aaaaatcctt
     4441 tacttcctta tcaatcgagt gtatcttttt atcatgttca atttggtact tatttaagaa
     4501 aaattctata agagtagtta agtcttctct tctctttctt agtggtggta tagttattgt
     4561 tattgtactt attctataaa aaaaatcttc tcttatattt ccatttgata tttccacttt
     4621 tggttcttta ttcatagcac aaattaacct aaagtcaact ttaatagatt tattacttcc
     4681 tatcctctct atagatttgt cctcaagtgt cctcaaaagt tttacctgaa catttagagg
     4741 tatatctcct atttcatcca aaaatagagt tcctttattc gaaagttcaa atctaccttt
     4801 tctactatct actgctcctg taaaagaacc tttttcatga ccaaacagct cagactccaa
     4861 tagattttct gaaaaagaac aacaatttac aggtacaaat atctcattct ttcttggact
     4921 tatgctatgt atatatctag ctaatatatc ttttcctaca ccagattccc ccaagattaa
     4981 tatattaaca tctttacaag cagctttttt tgctattttt atgatattat taaaatctct
     5041 atttttagat tccaaagtaa attcaaaatt atttttttgt acagaaacag atattttact
     5101 tgcctttacc ttttcaactt cttctaataa attttcaatg ggattacttt ttataaaata
     5161 agataaagct cctcttttca tagcatcaac tgcattttca atacttccat atcctgtaac
     5221 taatataacc tctatatctc tattaataga tttaattcta tctaatagct gtactccatc
     5281 catatttttc atcataacat cagataaaac caaatcataa tttttatctt ttttaattat
     5341 atttatagct tcttcaccac ttagtgctat atctacaaga tagcccttct tttgtagaat
     5401 ttttttcatt accactccat attctagctc atcatcaact aatagtattt tcattatata
     5461 gtcacctctt tatttaatgg aatttttata tgaaaacaag ttccaacccc caatttactt
     5521 tcaacactta tatctccatt attcttttta atctcattat aagtaatata taaacctaaa
     5581 ccagttccct caccaattgg ttttgttgta tagaagggat taaatatgtc ctttaggaca
     5641 tcttctttta ttccttcacc attatcttta aaatctataa ataaacaatg atttttttca
     5701 taacagtata taataatttt tccatcttga tgtattgcat ctattgaatt agaaattaaa
     5761 tttataaata catgttttaa cgattctcca tttatatagc atattaaatt atgttcacag
     5821 tcaattttta tttcaacatt ctttaactgt aacattttat attgaagttt cactatattt
     5881 tcaataaaat tttttatatt tatatgttct aaattatcat ctgatattct tgcgaaattt
     5941 aacaagttgg ttattatatt acttgcccta tctacattac tttctattga tttaacacaa
     6001 tcatttactt cttccattgt aacgttatct tttaagagat agcaattatt tcttattata
     6061 cccaaaggat ttcttatctc atgtgctacc ccagaagcta attgccctat agatatcatt
     6121 ttgttttctc taagtaattt cgcctctact attttatcat tagttatatc tttaataaga
     6181 actactatat actcagtatt atttttttta tactctacag gaaaagtgtt tatctttaaa
     6241 attttattgt gtatttctat ttcactagtt ttattagtat tttcagagaa ggtattttca
     6301 ataacacctt ttatcatgct aatcaaatct gctatcttat aactcatttc acccataaag
     6361 tcatatagta ctttgttcga ttctactata ttgttatttt catctacaag cattattata
     6421 tctgtaatac tattaaaagt tgcctctaaa gtttttttat tttctattac ctgttctgtt
     6481 ttccttttga tttctatctt taacaaatat gtatagctat aaaaaacata aattgatatt
     6541 aaaattatac ctataaaaag ataaatacta ggcacaattc ctcgctcata caatatatca
     6601 tcaacttcat tatacgtaga ataccatttt ttctttaaat ctttatacac tccatttttc
     6661 tgaagtttaa aaatgccttt atttaaaata ctaaccagct cttcatattg tttaggtaca
     6721 gcaagaactg cttttttagt atatatagga ttacttaaaa cactgtattt atttgaaagt
     6781 ccataattat ttatatagta tcgcaatact ggttcatcac caacaactgc gtctactttt
     6841 ccagacatta aatggttgac tccagtcttt atatctggag tcaataaaat gtttatatct
     6901 tgaatttttt gctttaaaaa atttatagaa taatcacctt ctggaattgc tactgttttc
     6961 ccctttaaat ccatgtaact atttatatct tgggactttt tatctttcaa tatatttgca
     7021 ctcaaagtgt atattgggtc agtaaatata aatttatttg ccctttcttt tgatggaatc
     7081 aagtcaaaaa actttatact atcatctatt gaatttacga aagattcttt ccaccaatta
     7141 tttggtttaa aataaaaatc tctaccaatt tgaattgaca gagaatttat taaatcaaca
     7201 ataattccct tatactgtcc atcttcctta cctttatatc tcaaaggagg tgaactttgg
     7261 tcagagctgt aaatcaaatt cttgtgtttt tctaaccact ttttttcttc ttctgtaaat
     7321 ggaagtgact tcttaatata ttcaaataca tttagattat actccatatt tacatacata
     7381 tttgtaagtg aaaatactac taaaaatgaa taaataatcc ctataataac aatctttttt
     7441 ttattcactt taaaactcca atcttctttt ttactataat aagttcaata tttaataaca
     7501 aaatcctact tttttcatta aatatttata tttattataa aactaattga atagatgttg
     7561 aaattatagt cattactatt cctatcaatg cttcatatgg aattaactta aatctttcac
     7621 ctatatccat gtttatacta cctgcactag tatgaaatag tgagccatgt ggtaattgct
     7681 cgaatacact agacccagca tttactattg atgcacctga aatcggtgat agcccaccat
     7741 ttattatagc atcatgaaaa gtagatgatg ctatagtagc accagctgtt gatgatgctg
     7801 ttgctaatga cataagtata cccgaaactg gtgccaataa aaattgtggc atatttaaaa
     7861 attgtaaagc acctattgta ctctgttgta attcagacat ctgtataatt cctgcgatag
     7921 tccctgttcc aagtaataat atgcataccc cttgcatctt agataatcca aaagttaaat
     7981 attctcttga attgttcaag tttcctgtga caattagagt tacgattcct cctattggaa
     8041 gggctatcat tgggtctata actataggag atacatttcc taaaaacaat agaaatatag
     8101 ctataattgg accacataat gatttaaata aacttggtaa atcttctctt tgctctaaaa
     8161 tttcatatga ttgtacttta tttcctttat ttattaatat acttgctaaa ataactgtaa
     8221 taacaactcc aataattgct ggaattatat ttgccatcat aacactagag agattgacag
     8281 aaaaattatc tgctactgct atagtatttg gatttggtga tattatatta cctgctttac
     8341 cccctcccaa catagctaat agtattgaca atttagaata atttaatttt ctacctatat
     8401 ataatcctat aggtgctaca gttattatag atacatccaa atttacacct actccagcaa
     8461 gtaccatagt tgaaatggca atcgaaaata aagccctctt ttcaccaaat atttttacaa
     8521 tctgttctga tattttatct actgcccctg tttttattaa acttccagct aatacacctg
     8581 atgccaaagc tctaagaatt gatggtgata tattcatagc tccatttgcc ataaaatcta
     8641 cagtttgtgt tatatttgct cctccaacta atcctccaat aaatgctcct aacattaaac
     8701 tatatacagc ttgaaatttt tttataatta aaataattgc tattaataaa ccaaatatag
     8761 ctccaaaagt cgttatttgc ataatatccc ctcctatcat aaattaacta tcttttgctt
     8821 aattttattt aaatatattt taagtattct gtatccactt tgtcaattat attttaattt
     8881 atttaattat attgaacata gttttttcaa aacctaattc tatatgttat aataatttca
     8941 tactacataa tcattttact tgattatttt aagttaatat tttttaaatt aaaaagagag
     9001 gatatttata tgaatgattt aaatttaggg gaaaaaatag ctgaagttcg aaaaaaacaa
     9061 aacttaagta ttcgagattt agcaaaacta gctgatgtta caccatcatt attgagccaa
     9121 atagaacgag gtcttgctaa tccatctgta aattcattaa aatctatagc ctcttctcta
     9181 aatgttcctt tatttacatt ttttgtatct gaagtagata aaaaaaactt gatagtaaga
     9241 catgataata gaaaaaaagt tattttacct ggaagcaaag aagttatata tgagatttta
     9301 attccagact ctagtggtaa tttagaattt gcaattatgg atttagctcc aaacacttct
     9361 tcttgtgttg ataggattac tcataatgga gatgaaattg catatgtttt ggagggagaa
     9421 gttaaacttt ttatggatga tgatgaattt actttgtcca aaggagatag tgtaaaagtt
     9481 cctttaggaa caaaacataa atggcaaaat gactcaaata gtgaaagtaa agtaattttt
     9541 gcagtcatct tgtaaaggag taaaaaccat gaagacatat aaatttatat tttttgatgc
     9601 agatgatact ttatttgatt ttaagaaaag tgaatcacat gcttttaaaa aacttctatc
     9661 tgagtttgat ttagaattta attttgagaa ttatatagag tcatatagaa atataagtga
     9721 taagctttgg ctcgaccttg aaaaaaatat tataactttg aatgaactta aattacttag
     9781 atttgaactt tttgcaaata aaatatcttt ggatgttgat tctgaaactc ttagcaaaat
     9841 gtacttaaat tttttaggtg aatgtacatt tttaattcca ggtgcaatcg atatattaca
     9901 atatcttaaa aaaaagtata ctattgttat aataacaaat ggaatttctg cagttcaaaa
     9961 taaaagactt gaaaattcaa aaataaaagg atatattgat ggaatggttg tatctgaaga
    10021 gttgaaaata tcaaaaccaa atcctgaaat atttaaatac gctctcaaaa agtttaattg
    10081 tcatgataaa tcttctgcct taataattgg ggatagctta acctctgatg tcttaggtgg
    10141 aataaattca ggaatagata cttgttggtt aaattccaat aacagtataa attatactaa
    10201 tcatatacca acttatgaaa taaatacact tattgaatta aagaaactac tatagtttgt
    10261 aagtctgttc aaattaaata gaatattact ttgcaataat ttttttagtt tatacataaa
    10321 atgaccaaaa aaattattgc aatttataca caaaacaaat cttagtcttg aaaattaact
    10381 aaaagcattt tctaggacac ttactacatc ttgattctaa attttcttca tcccccatta
    10441 aaataagcat tgcaacactt tttatggggg atagttgatg ataatcatta atttctattt
    10501 ctatactatt atatttattt tttacatagt tataaatatc cttttgtctc tctacttcaa
    10561 aatctttatc tcctggatat atttcatgcg atattttgag actatcatac tttcctttta
    10621 aatacatttt tattttctca ttaatataat ctaaacatac tatacctatt ttatccaatg
    10681 ctagccctgc catcatattg caattttcca tatagtcatt catcttaata tcaattttat
    10741 cccctattgt atatagtaca gcaaatgctt ttttacattc tttaggtgtt ttattaatat
    10801 caacttcact tatatatatc ttgatatcta aaatatcatt tatatttctt atttctcttt
    10861 caacgatttt actaattgta tctggagctt ttctatttcc ataccccaaa aactttagca
    10921 caatattctt atctataaaa atatcatcta aatttatatc ctttaaaaga tatttatttt
    10981 tattactatt ataattcatc aatatcaatc ctatctaatt ctttataact aattcttcct
    11041 gcctttatag ctgcttgtaa attttctact ggagtggtat ttaagcagtt acagccaaaa
    11101 tcacataaaa agttatcata tcttctcatc ttctttaaca attttagcgt ctctcttcta
    11161 atcttatctg gcttagaacg tccaagaagt tttattgggt ctaaattacc aatcaaaact
    11221 atatctctag gaatcattgg agcaatttct tcatagtcta aaatttggtc taaacttact
    11281 gcatctatat tacaacttag catattatct aataattctc tggtgtctcc acatatatgc
    11341 atacctttcc aacaatcaag ttcattgtag attttattta aattttttac tataaattca
    11401 tcaaatctat ctttacttag ggttactgat gttggctctg ctattgaaat atattttgct
    11461 cctgctttat ttgcagctac agcatatctt cttaccaatt cagttgtaaa ttccaataat
    11521 tcttctacaa attttttatc agttattata catcttaaaa gttgagtagc tccagctaat
    11581 tgacctgcaa gtgtaaatgg accttgtatc gattcatata gtggtttttt aatacttttt
    11641 gatataactg ataagctctc tatattgagt ggcatacgtc cactcgtata agggtctgga
    11701 acatctaatt ttcttaattt atctctgtca gttatagtat gtgtcaatac acttggaaaa
    11761 tcataatctg gtcttaatgt ctctaaaccc agagtttcac aaaatgtaat cccatcacag
    11821 aatgagtata taaaatctga ttcaaaatac ttgtccatag tctttgctaa ttctaattgc
    11881 aaatctacac tatcatatac ttcatatgcc tttttattta ttaagaacaa tccatttgta
    11941 cccatatcag gcagaaaaaa acctctatct tcactattta tatactgaag taaagtcata
    12001 tctgaaaaca tttaaacttc acctcctata agtttattta ttaatttaac tgcttttgga
    12061 gcatttgaac tgtatccatc tgctcctatt tcttctgcaa actccatact tacaggtcct
    12121 ccaccaatta ttacttttgg tcttttatct atctcaatca tattaagttc gtctataacc
    12181 gatttcatat tttccattgt agttgtcatc attgaagata agcctattat atctacattg
    12241 ttttttatag cttcttctat tatatcttca ctttttctat ttacacctaa gtcaataacc
    12301 ttatatccag tggcttctac cattactttt acaatatttt ttcctatctc atgagtatct
    12361 ccttctacta cagacatcag aattactccc tttgattttt tctttacttt tccagttccc
    12421 ttcaaaagat taataccttt atttaatgca tctgtgcata ctataacttc tgaaagatag
    12481 tattccttat tctcaaactt atctaaagct cctatcatac ctttgtttaa accatattca
    12541 taaatatctt ccacatcaat atttttatct atagcaattt ttactaaatt ctctatatta
    12601 tctacaccca tatttaatat acattctgct atatcatcaa gtatgtccat ataatactca
    12661 tcctttctaa ttacttacaa aattgctttt tcaatatcca ataatttctc ttatttaaca
    12721 aggactaact ctaggtatca atcaatttta tttaatatca tctaaaacta ttgataattt
    12781 aaatttgcag tccatggaaa atctacagga aatgcctttg tagcaaattc atatatattt
    12841 tttagatgtt taaattcatt gtactgttcc tcattttttt caaaaaacag tttattaagc
    12901 ttatccaaat tttctattac aagttcttct tctttgtatt taataatata tttattttca
    12961 acttgtttaa attccatgta ttttagtaat tcaaaatcta catattgact aaaataactt
    13021 cttaaactat cacacaactt agtaaaattt attattttca tagttccttg ttgataatcc
    13081 aaagattttg tcccattaca tttcaactgg tctttaagat ttttcacatg tactttataa
    13141 tttaaatatt ctaatccaaa tttatttgca acatatttca atatattttc aacatttaca
    13201 gagtttaacc ctatttcagc aacttctcct acagtagaat cttctttttt tattgttctt
    13261 attatcaaat atcctattat attattattt tcctttataa ataccttctt gtaaccaatt
    13321 ggtccccagg caatagttgc tgcatgcaat agtttttgaa actcatctct cgttcttata
    13381 aatctagtac tattttgatt atatagttca atcattttat ctaaatcatt atctttttca
    13441 aaattagttt catcaaattc aactatttca tatgctattt ttacatcttc aggttttatt
    13501 gtatatttat aaaaactctt taccaaacta caatttcttc tactgtatag acttcttgtt
    13561 cctgatataa ttacaatatc tacaccatca taaaacattt tttcttctac tttatccaat
    13621 atcttagatg aatagcctct cttttcataa tctggatgag tacatactcc acctatagct
    13681 gcaaccttca atctgtttcc ttgaatagat acatcctgaa ttagatagtt tacatcagaa
    13741 atcactttat catcttctga tattataatc atattttcta tattattttt acttagtaaa
    13801 agaggaaatt cttccatcat agttggttta tgatttcttg atattctaaa tacatagttt
    13861 attaattcta ttactttatc aaattcttct aattttgcac ttcttggtct taattccatt
    13921 catattcctc caaatattat ttaagatact taaaactcta
//
LOCUS       DAMJYR010000099        13895 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.102, whole genome shotgun sequence.
ACCESSION   DAMJYR010000099 DAMJYR010000000
VERSION     DAMJYR010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13895)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13895)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13895
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.102"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            269..1462
                     /gene="nifV"
                     /locus_tag="PC471_002785"
     CDS             269..1462
                     /gene="nifV"
                     /locus_tag="PC471_002785"
                     /EC_number="2.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocitrate synthase"
                     /protein_id="HDF2789897.1"
                     /translation="MCVISKDRAKEIKIVDTTLRDGEQTAGVVFANREKIMIAEMLSD
                     LGVDQIEVGIPTMGGDEKNVIKHICSRNLKSDIMAWNRAVIKDVEESISCGVDAVAIS
                     ISVSDIHIENKLRTSRGWVLENMAKTVEFAKKNGLYVSVNGEDASRADIDFLTEFINV
                     GKQAGADRFRYCDTVGVMNPFSIKNAIETLYERTNFDIEMHTHNDFGMATANALAGIA
                     AGANYVGVTVNGLGERAGNAALEEVLMALKCVYKCDLNNIDTRKFRGICEYVAQASGR
                     ILPTWKPVVGDNMFIHESGIHADGALKDPHNYEPFDPSEVNLERKIVIGKHSGRAAVV
                     NKLSEYEMYISPENATKLLNAIRATSIRLKRSLMDKEILQLYCDILAHEKGTTEEEAV
                     RGSYI"
     gene            1487..3412
                     /locus_tag="PC471_002786"
     CDS             1487..3412
                     /locus_tag="PC471_002786"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HDF2789898.1"
                     /translation="MGDNIVYKIIKKHIVDGEAVAGSSIGIKIDQTLTQDSTGTMTYL
                     QLEAMGIDKVKTKRSVAFVDHNMLQQGFENADDHKYIQTVADKYGVYFSKPGNGICHQ
                     VFLERFSTPGDTLLGSDSHTPTAGGVGMMAIGAGGLDVALAMAGGAYYIKAPKVCKVN
                     LVGKLNNMVSSKDIILEVLRKQTVKGGVGKVYEYGGEGVKSLSVPQRATITNMGAELG
                     ATTSIFPSDEKTLEFFKSQGREDAWVELKPDADAVYDEEITINLDELKPLAAKPHSPD
                     NVDEVENIGKIKIDQVAIGSCTNSSYEDLMKVAQILKGNKVHKDVSLVIAPGSRQVME
                     MIARNGALADIISAGARILENSCGPCIGMGQSPGTDSVSLRTFNRNFYGRSGTLSAQV
                     YLVSPEVAAVSAIKGVLTDPREFDIKFTNLDVNEFLIDDSMIIKPADVGSDVEVVRGP
                     NIKPFPLNTELSQSIGGKVILKTEDNITTDHIMPSNAKLLPFRSNIPYLANYCFNTVD
                     TEFPQRAKDNNGGFIVGGDNYGQGSSREHAALAPLYLGVKGVIVKSFARIHKANLINS
                     GIIPMEFCDEKDYENISLLDNLEIPNILDNLGSGILEVKNTTKGTSFKVKVELSAKEV
                     DVLKAGGKLNYTKNQAN"
     gene            3434..4429
                     /locus_tag="PC471_002787"
     CDS             3434..4429
                     /locus_tag="PC471_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate dehydrogenase family
                     protein"
                     /protein_id="HDF2789899.1"
                     /translation="MYKVTLIPGDGIGPEVAKAMKKVVEATGVEIEWEEVNAGEAVIE
                     EYGTPLPEYIIDSIKKNKIAIKGPITTPVGKGFRSVNVTLRQALDLYVNLRPIKSFKG
                     IKSRYEDVDLVVVRENTEDLYAGIEHKIGDYAAESIKIITRSASERIVDFACNYVKDN
                     KRKKVTAIHKANIMKMSDGLFLDVFREVASKHGVEYDDLIVDAAAMNLVLNPENYDVM
                     VMPNLYGDILSDLGAGLVGGLGIIPSANIGKDCAIFEAVHGSAPQIAGQNKANPTALI
                     QSSVMMLRYLGEYENAQKIETALEKVFLEGSKLTVDLGGSASTTEFADEVCKYIV"
     gene            4754..5227
                     /locus_tag="PC471_002788"
     CDS             4754..5227
                     /locus_tag="PC471_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDF2789900.1"
                     /translation="MVVSVEVCLIKTLDSNILREVGTLSRAVNSINDIKYKELKLQKG
                     QFTFLTRICENPGINLVELSNMLKVDKATTTKAIQKLIKAGYVDKKQDKFDKRGYNLT
                     PTDKSLEVYELIIEEENRSIEICFDNFTDEEKQVVTKLLEKMSKNVENEWFKVKR"
     gene            5261..5755
                     /locus_tag="PC471_002789"
     CDS             5261..5755
                     /locus_tag="PC471_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDF2789901.1"
                     /translation="MIRKANMNDLESIMKIIKSTVEEMKTYNNTQWDENYPLEKDFVS
                     DIKKQDLYIYEVDGEVAGFICLNYEEPEEYLGLNWSSNKKAMVIHRMAVNPNFRKTGI
                     ASKLVDFAEKLAVENNVSYLKSDTYSINSKMNLLLTKCGFIKIGEMSFLGKEKSFYCY
                     DKIL"
     gene            5852..6373
                     /locus_tag="PC471_002790"
     CDS             5852..6373
                     /locus_tag="PC471_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HDF2789902.1"
                     /translation="MIGFLKKRRSIRKYKDVEVEKEKLDKILKAALLAPSSKGLRTWE
                     FIVVDDKEKLINLSQCRTKGGGFFLKNAPLAIVIIADKEKNDVWIEDASIAASYIQLQ
                     AHELGLGSCWIQVRNRMHDDNIEADKYIREELKVPSKYSVECIISIGYPDEEKKAYGD
                     SDLDYKKVHFNNF"
     gene            6562..6873
                     /locus_tag="PC471_002791"
     CDS             6562..6873
                     /locus_tag="PC471_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HDF2789903.1"
                     /translation="MGIFKTKMDEDWKVNYIKEFNEMRDSYESKLQKKQFEVDSLKSE
                     LDRLRSYKNSLKPKEKQITDDDINNIKSLRRDGLSYKEISNQTSWSKATVSRVLNGLY
                     D"
     gene            6901..7587
                     /locus_tag="PC471_002792"
     CDS             6901..7587
                     /locus_tag="PC471_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HDF2789904.1"
                     /translation="MKYSYKKCIIDSILLMFIVQILRMILNYVLLSQFEFTLENFNII
                     NLISFTLVGLSLILFLKDNSLYNKVRNRKITEAFEENKNNILIEKCKLILFVVVLSLA
                     IIVTYCTKGYVLFNVTMMTLSVLIVPIFEELFFREYIWNYLSNFIKSKSKIICITSIL
                     SGIYNIGYIDVIRNYVILYNNSSYTFEVIISKIMIGTVFGIVLGLVKYRFRDVGFCIL
                     LRSLFAIFIR"
     gene            7819..8703
                     /locus_tag="PC471_002793"
     CDS             7819..8703
                     /locus_tag="PC471_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HDF2789905.1"
                     /translation="MKFVTFCSSNEEKIGVFNSETNSIYEINSLGLSKLYTDMNDFIE
                     NVSTGDLEKIKNNSFENAKCYKLEEVKLCSPIVRPKKDIICLGLNYKDHVNEIPDGVI
                     KNVVMPDYPIYFSKRADKIIGVDDKISLHGDLVEKLDYESELAVIIGKEGINISKEDA
                     YEYIFGYTIVNDISERALQDKHVQWFRGKSLDTHTSMGPCIVHKEEFEHPLKLDISSV
                     VNGEVRQDSNTEYFIFDIPTIINDLSRGMTLKPGDIISTGTPAGVAMGMNPQVYLKHG
                     DIVECKVEGIGVLKNIVD"
     gene            8802..9977
                     /locus_tag="PC471_002794"
     CDS             8802..9977
                     /locus_tag="PC471_002794"
                     /EC_number="2.1.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolecarboxamide
                     formyltransferase"
                     /protein_id="HDF2789906.1"
                     /translation="MARELELKYGCNPNQKPSKIYMKNGELPIEVLNGKPGYINFLDA
                     FNSWQLVKELKEATGLPAATSFKHVSPAGAAVGVPLSDTLKQIYFVDDLELSPLACAY
                     AMARGADRMSSYGDFIALSDVCDKETATIIAREVSDGIIAPGYTDEALEILKGKRKGN
                     YNIVKIDENYTPEPIETKDVYGITFEQGRNEILINEDLLKDIPTDNKIFTDSAKRDLI
                     IALITLKYTQSNSVCYAKDGQVIGVGAGQQSRIHCTRLAGNKADTWYLRQHPKVLNLK
                     FKKDIGRPDRDNTIDVYLSDDYMDVLADGIWQNFFEEKPEPLTGEGKRAWLKTLTGVA
                     LGSDAFFPFGDNIERAKRSGVSFIAQPGGSIRDDNVILTCNKYNIVMAFTKNRLFHH"
     gene            10105..10959
                     /locus_tag="PC471_002795"
     CDS             10105..10959
                     /locus_tag="PC471_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2789907.1"
                     /translation="MALNIRNYSLLCNQGTAKFNEDVVGINPFGAWVLDGATGLNGKN
                     LVSTESDARWYVQWWNRYLYKNINKEESLKSIINTGIIEVKKEYELRLNGFKVEKLDL
                     PSSSIVVVKFHENKVEYFLLGDCALFIKNGDSKVIKDRSICKFDNTVFDEMSKLSNLE
                     EMAFDEIRTSVMDTIVSNRLKKNTKEGYWILDFDEEAVENSIYGYIEIKKDFQLMLTS
                     DGFTSACDRYNLIKEEELIRIAEKFGIGYIHNKVRDFEDNDYKAVKVPRFKVKDDSSC
                     LYLDIYND"
     gene            11003..12238
                     /locus_tag="PC471_002796"
     CDS             11003..12238
                     /locus_tag="PC471_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HDF2789908.1"
                     /translation="MKILHIITQKPNSTGSGIYLSGMIKGFEKIGHKQAVIAGIDVND
                     DVNCFPSEVSFYPVKYNCGELNFPVVGMSDSMPYESTRYKDLNIDMINRLKYQFKVNI
                     DKAMNDFKPELIICHHLYLLTAFVREMVKDIKVMSICHGTCLRQLNTIDLEKEYIIAN
                     IRKLDLIFALHENQKYDIIKTFGVSESKVVVIGSGYNDDIFYNKNYKIKDDKIKIVFA
                     GKICKSKGLIPFIKAISKLKYSKDLIEVNFAGTGSDIESYNEIVKLASKSPFKMNFLG
                     KLEQRDLAELFNRSQIFVLPSFYEGLPVVVLEALSCGTDVITTDILGVKEWIGSEINN
                     SGKIEYVSLPFMEKEGIPKDEELYDFENNLYNAIDSKIQSLLNNSNKKTSVDMSKKTW
                     DGLAYRINEVILMDELCLV"
     gene            12339..13280
                     /locus_tag="PC471_002797"
     CDS             12339..13280
                     /locus_tag="PC471_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp25"
                     /protein_id="HDF2789909.1"
                     /translation="MRISKKVLALGISALLLSVSFPTSINALDKIENIQGVDKYETAG
                     LISDKQDFTTAVLINADSTMVDGIAASGLAGVNNAAILLTNKDDIPEATLQRLNRVTK
                     IYVIGGENSISKDVEKMLLMRRMQVIRIDGVDRVDTSYKIAGEIEKIKNSDKMFLVNG
                     FKDEADAVSVASVAYRDGAPIILTKDIPSAEEDTDLDPWFGVSPVPVYAIGGESTLSD
                     YIVSRYRATRIGGVDRYQTNKNVIEKFYNGAKEFYITSGDDLVYALVASPLAKNAPVV
                     LVSNKSDKSILSGASKVTAIGISDKSIIEQCLDAVKK"
     gene            complement(13499..13852)
                     /locus_tag="PC471_002798"
     CDS             complement(13499..13852)
                     /locus_tag="PC471_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HDF2789910.1"
                     /translation="MDRKRELKQLYKEMKFDTGVFIIKNDITKKIYLGKSNDIKSKFN
                     SLKFQLGAGSCMIKELNNEWKKYGEKAFTFEVLELIKHDDNKTEKDYLEELDILEMVW
                     LEKLKEDNTYEIYSI"
BASE COUNT         5488 a         1376 c         2616 g         4415 t
ORIGIN      
        1 aaataaatct atttaaaaat taaattaaaa tgcaagaaaa acataataac tttcattttt
       61 aagaagtttt cacaaaaaag aaaaaatata gtgcaataaa tgatacaaag taatataata
      121 gattacaaga tatgaaaagc taaaaaaata aattgcaaat gaaattaaaa tgaataaaaa
      181 ataaaaaaat aatcataagt tagaagtatc aatgaaaaat aagacttaat gtttttaaaa
      241 ttaaaagtat actaaggggg gcattaatat gtgcgttata tcaaaagata gagcaaagga
      301 aataaaaata gtagatacta ctttaagaga tggagagcaa acagctgggg ttgtatttgc
      361 aaatagagaa aaaataatga ttgctgaaat gttaagtgac ctaggtgttg accaaattga
      421 agttggtata cctactatgg gtggagatga aaaaaatgta ataaagcaca tatgtagtag
      481 aaatttaaaa tcagatataa tggcttggaa tagagcagta ataaaagatg ttgaagaatc
      541 tatttcttgt ggtgttgatg cagtagctat atcaatatca gtatctgaca ttcatataga
      601 aaataaattg agaacttcta gaggctgggt tttagaaaat atggctaaaa cagttgaatt
      661 tgcaaagaaa aatggattat atgtttcagt aaatggagaa gatgcttcaa gagcagatat
      721 agacttctta actgaattta taaatgttgg aaaacaagca ggggcagata gatttagata
      781 ctgtgataca gtaggagtta tgaatccttt ttctataaag aatgcaatag aaactcttta
      841 tgaaagaact aattttgata tagaaatgca tacacataat gactttggga tggcaactgc
      901 aaatgcactt gcaggaatag cagcaggagc aaattatgtt ggtgttacag taaatggatt
      961 aggtgaaaga gcaggaaatg cagcattaga agaagtttta atggcactta agtgtgttta
     1021 taaatgtgat ttaaataata tagatactag aaaatttaga ggaatatgtg agtatgtagc
     1081 acaggcatca ggaagaatac ttcctacatg gaaaccagta gttggggata atatgtttat
     1141 acatgaatct ggtatacatg cagatggagc tttaaaagac ccacataact atgaaccatt
     1201 tgacccaagt gaagtaaatc ttgaaagaaa aatagtaata ggtaaacatt caggaagagc
     1261 tgcagttgta aataagttga gtgaatatga aatgtatatt tctccagaaa acgctaccaa
     1321 gctattaaat gctataagag ctacatctat aagattaaaa agaagtttaa tggataaaga
     1381 aattcttcaa ttatattgtg atatactagc acatgagaaa ggcacaacag aagaagaagc
     1441 agttagaggt tcatatattt aagatataat tgtttaggag gaaaatatgg gagataatat
     1501 agtttataaa ataattaaaa aacatatagt agatggagaa gctgttgctg gaagttcaat
     1561 tggtataaaa atagaccaaa ctttaacaca agattctact ggaacaatga catatcttca
     1621 actagaagct atgggaatag ataaagtaaa gactaaaaga agtgtggcat ttgtagacca
     1681 taatatgtta caacaaggat ttgaaaatgc agacgaccat aagtatatac aaacagtggc
     1741 agataaatat ggagtttatt tctctaagcc aggaaatggt atttgtcatc aagtattttt
     1801 agaaagattt tcaacaccag gagatacact tttaggttct gatagccaca cacctactgc
     1861 tggaggtgtg ggcatgatgg ctattggagc tggtggatta gatgttgctt tagccatggc
     1921 aggaggagca tactatataa aagcaccaaa agtctgtaag gtgaatctag ttggaaaatt
     1981 aaataatatg gtatcatcaa aagatataat attagaagtg ttaagaaaac aaactgtaaa
     2041 aggtggagta ggtaaagttt atgaatatgg tggagaaggt gtcaaaagcc tatctgttcc
     2101 acaaagagct acaataacaa atatgggggc tgaacttgga gcaactactt caatcttccc
     2161 aagtgatgaa aaaacactag aattttttaa gagtcaagga agagaagacg catgggtaga
     2221 gctaaaacca gatgcagatg cagtttatga tgaagagatt actataaact tggatgaatt
     2281 aaaaccatta gctgcaaaac ctcatagccc agacaatgta gatgaagttg aaaatatagg
     2341 caagattaaa atagaccaag tggcaatagg aagttgtaca aattcttctt atgaagactt
     2401 aatgaaagta gcacaaatcc tgaaaggaaa taaagtacat aaggatgtga gtttggtaat
     2461 agcaccaggt tcaagacaag ttatggagat gattgctaga aatggggctt tggcagatat
     2521 aataagtgct ggagctagaa tactagaaaa ttcttgtggt ccatgtattg gtatgggtca
     2581 atctccaggt actgactctg tgtctttaag aactttcaat agaaatttct atggtagaag
     2641 tgggactttg tcagcacaag tatatttagt aagtccagaa gtggcagcag taagtgcaat
     2701 caagggagtg cttacagacc caagagaatt tgatattaaa tttacaaatt tagatgtaaa
     2761 tgagtttttg atagatgata gcatgataat taaacctgct gatgtaggaa gtgatgtaga
     2821 agtagttaga ggacctaata taaaaccttt ccctcttaat acagagctaa gtcagtctat
     2881 aggtggaaaa gttatattaa agacagaaga taatataact actgaccata taatgccatc
     2941 aaatgctaag ttacttcctt ttagaagtaa tataccgtat cttgcaaact attgtttcaa
     3001 tactgtagat acagaatttc ctcagagagc aaaagacaat aatggtggat ttatagttgg
     3061 tggagataat tatggtcaag gttcaagtag agaacatgca gctttagcac cattatatct
     3121 aggtgttaaa ggggtaatag ttaagagctt tgcaagaatc cacaaggcta acttgataaa
     3181 tagtggtata attccaatgg agttttgtga tgagaaagat tatgaaaata tatctctttt
     3241 agataatctg gagataccaa atattttaga caatctaggt agtggaattt tagaagttaa
     3301 gaatacaact aaaggaactt cttttaaggt caaagtagaa ttgtcagcaa aagaagttga
     3361 tgtattaaaa gctggtggaa aattaaacta cactaaaaat caagctaatt aatatgaaaa
     3421 gagggaatat tgaatgtata aagtgacact tataccagga gatggaatag gaccagaagt
     3481 tgcgaaagct atgaaaaagg tagtagaagc tactggagta gaaatagaat gggaagaagt
     3541 taatgcagga gaggcagtta ttgaagagta tggtactcca cttcctgaat atattataga
     3601 tagtataaaa aagaacaaaa tagctataaa aggcccaatt actactcctg taggtaaagg
     3661 atttaggagt gtaaatgtaa ctttaagaca ggcattagat ttgtatgtca acttaagacc
     3721 tattaaaagt tttaagggga taaaatcaag atatgaagat gttgatttag tggttgtaag
     3781 agaaaataca gaagatttat atgctggaat agaacataaa ataggtgatt atgcagctga
     3841 aagtataaag ataataacaa gaagtgcaag tgaaagaatc gttgattttg catgtaacta
     3901 tgttaaagat aataagagaa aaaaagttac agctatacat aaggcaaata taatgaaaat
     3961 gtctgatgga ttgtttttag atgtgtttag agaagttgca tcaaaacatg gtgtagaata
     4021 tgatgatttg attgtagatg cagcagctat gaacttagtt ttaaatccag aaaattatga
     4081 tgtaatggta atgccaaatc tttatggaga tatactatca gatttaggtg ctgggcttgt
     4141 tggaggttta ggtataattc caagtgcaaa tattggtaaa gattgtgcga tatttgaagc
     4201 tgttcatgga agtgctccac aaatagcagg tcaaaataag gctaatccaa cagcacttat
     4261 tcaatcatca gtaatgatgc ttagatactt aggtgaatat gaaaatgcac aaaaaataga
     4321 aacagcttta gaaaaagtat ttcttgaagg aagtaaattg actgtagatt taggtggctc
     4381 agcttctact acagagtttg cggatgaggt gtgtaagtat attgtataga ttttatttag
     4441 ggaaaaagta aaatatagat tgaagaaaag aggctttctc aaaatagaga taagcctttt
     4501 ttattggaat gataaaatat atttgatttt atacaaaaga gagttcctca taaacgaaaa
     4561 agttaatttt gggatatcca ttttaatttt aaaattaatt gattaatacg caaagaaaga
     4621 ttaaataaat ttattatttt taattgaact ttcaaccaaa atatggtaaa atttaaaaag
     4681 ttgaactttc aactaaaaat gaaaatattg attatgtgaa attctataaa acagaaacta
     4741 tttgacttta gccatggtag tttcagtgga ggtgtgtttg attaagactt tagatagtaa
     4801 tatattaaga gaagtaggta ctttatctag agcagtaaat tcaataaatg atattaagta
     4861 caaggaatta aagttacaaa aaggacaatt tacatttttg acaagaatct gtgaaaatcc
     4921 tggtataaat cttgtggaat tatcaaatat gttaaaggta gacaaggcta caacaaccaa
     4981 ggctatacaa aaattaataa aggcaggata tgtagataaa aagcaggata agtttgataa
     5041 aagaggatat aatttaactc caactgataa atctttggaa gtttatgagt tgataattga
     5101 agaagagaat agaagtatag agatatgttt tgataatttt acagatgaag aaaaacaagt
     5161 ggtaactaag ttattagaaa aaatgagcaa aaacgtagaa aatgaatggt ttaaagtaaa
     5221 gagatagtaa ataaatacat taacttaagg agatattttt atgattagaa aagcgaatat
     5281 gaatgattta gaaagcatta tgaaaattat aaaatctaca gtggaagaaa tgaagactta
     5341 taataataca caatgggatg aaaactatcc attagaaaaa gactttgttt cagatattaa
     5401 gaaacaagat ttatatattt atgaagtgga tggagaagta gcagggttta tatgtcttaa
     5461 ttatgaagag ccagaagaat atttaggctt aaattggtca tctaataaaa aagcaatggt
     5521 aatacataga atggctgtaa atccaaactt tagaaagaca ggaatagcat ctaagttagt
     5581 tgattttgct gaaaaattag ctgtagaaaa caatgtatca tacttaaaga gcgatacata
     5641 ttcaataaac tcaaaaatga atttattact tacaaagtgt ggttttataa aaatcggaga
     5701 aatgagtttt ttaggaaagg agaaatcttt ctactgttat gataaaatat tataaagagc
     5761 atttataata caaggggtaa ataatagatt ttcagcatta gtaagggcat aatataacct
     5821 agtatatgct attatgaaag ggatgattta tatgattgga tttctaaaga aaagaagaag
     5881 tattagaaaa tacaaggatg ttgaagtaga aaaagaaaaa ttagataaaa tattaaaagc
     5941 tgctctttta gctccatcat caaaggggct tagaacatgg gaatttatag tggtagatga
     6001 taaagaaaag ctaattaatt tatctcaatg tagaactaaa ggtggtggat ttttcttaaa
     6061 aaatgcacct ttagctattg taattattgc agataaagaa aaaaatgatg tttggataga
     6121 ggatgcaagt atagctgctt catacataca acttcaagct catgaactag gtttaggttc
     6181 ttgttggata caagtaagaa atagaatgca tgatgataat atagaagctg ataagtacat
     6241 tagagaagaa ttaaaagtac ctagcaagta cagtgtagaa tgtataattt ctataggata
     6301 tcctgatgaa gaaaagaaag catacggtga ctctgattta gactataaaa aggttcattt
     6361 taacaacttt tagtatataa tatgttgtat taagacatta tatttttttc tattgttaat
     6421 atatacttaa aattgtaaaa atatatctga attttactaa cttatctagg ggttatgtac
     6481 aaggatttta aagataaata tagaaattaa tgtttataat cgatacataa cttgagtaac
     6541 aaaatttaag gaggttaaag tatgggtata tttaaaacaa aaatggatga ggactggaaa
     6601 gtaaactaca ttaaagagtt taatgaaatg agagattctt atgagagtaa acttcaaaaa
     6661 aaacagtttg aggtagatag tttgaaatca gaattggata gactaagaag ctataaaaat
     6721 agccttaagc caaaagaaaa acaaattact gatgatgata taaataatat aaagagttta
     6781 agaagagatg gcttaagcta taaagaaatt tcaaatcaaa caagttggag caaagcaact
     6841 gtaagtaggg tattaaatgg actttacgat tagcatatac atggggtgat ttaaattggg
     6901 atgaagtata gttacaaaaa atgcataata gattctattt tattgatgtt tatagttcaa
     6961 atattaagga tgattttgaa ttatgtactt ctaagtcaat ttgaatttac tcttgaaaat
     7021 tttaatatta taaacttaat ttcttttaca ttagttggtt tatcgttaat tttattttta
     7081 aaagataatt ctttgtataa caaggttaga aatagaaaaa tcacagaagc ttttgaagaa
     7141 aataaaaaca atatacttat tgaaaagtgt aagctgatac tttttgttgt ggttttatct
     7201 ttagcaataa ttgtaactta ttgtactaag ggatatgtac tttttaatgt aactatgatg
     7261 actttgtctg tattgatagt cccaatattt gaagagttat tttttagaga atatatatgg
     7321 aactatctaa gtaattttat aaagtcaaag agtaaaatta tctgtataac atctatacta
     7381 tctggaattt ataatatagg atacatagat gttataagaa attatgtaat attgtataac
     7441 aattcaagct acacctttga agttattatt tcaaaaatta tgataggtac tgtttttggt
     7501 attgtacttg gattagttaa gtataggttt agagatgtag ggttttgtat attattgagg
     7561 agcttatttg ctatatttat taggtagata gtataatcgg tttataaggt tgttttaaat
     7621 ttaattttaa atcaacctta ttttatttgt gttttataaa gcacttatat attaataact
     7681 aataaattta ataaaaatta tttgaatatt tttaaaatat ataaatatgt attgacatat
     7741 aaatattatg aattacaatt tatataaact aataaaaatt aaataatatt aatcaactta
     7801 ttgaatggag gaataaaaat gaaatttgtg actttttgta gtagcaatga agaaaaaata
     7861 ggtgtattta attcagaaac aaatagtata tatgaaatta atagtttagg attaagtaaa
     7921 ttatatactg atatgaatga ttttattgaa aacgtatcta caggagattt agaaaaaata
     7981 aaaaacaatt catttgaaaa cgcaaagtgt tataagttag aagaagttaa attatgttcc
     8041 ccaattgtaa gacctaaaaa agatattata tgtttaggtc taaactataa agaccatgtc
     8101 aatgaaatcc cagatggagt tataaaaaac gtagttatgc cagattatcc aatttatttt
     8161 tctaagagag cagacaaaat aattggagtg gatgataaga taagcttaca tggagatttg
     8221 gtagagaagt tggactatga atcagaactt gcagtaatca taggtaaaga aggtataaat
     8281 atatcaaaag aagatgctta tgaatatata tttggatata caatagtaaa tgatataagt
     8341 gaaagagcac ttcaagataa acatgttcaa tggtttagag gaaaaagctt agacacacat
     8401 acatctatgg gaccttgtat agtacataaa gaagaatttg aacatccttt aaaattagat
     8461 ataagtagtg tagtaaatgg agaagtaaga caagattcta atacagagta ctttatattt
     8521 gatataccta ctataataaa tgatttatca agaggaatga ctctaaagcc aggagatata
     8581 atttcaactg gaactccagc aggggttgct atggggatga atcctcaagt atatttaaag
     8641 catggcgata tagttgaatg taaagtcgaa ggaataggag ttttaaaaaa tatagttgat
     8701 taatggataa atatgccata taattttatt agtacgaata atttaattaa tataggatta
     8761 atcaaactta tgattaattt attatagaga gggatgagta tatggcaaga gaactagaat
     8821 taaagtatgg ctgtaatcca aatcaaaaac catcaaagat ttatatgaag aatggagaac
     8881 ttcctataga ggtattaaat ggaaaacctg gatacataaa ttttttagat gcatttaata
     8941 gttggcaact tgtgaaggaa ttaaaagaag ctacaggtct tccagcagca acttcattta
     9001 aacatgtaag tccagcagga gcagcagtag gggtaccttt atcagatact ttaaagcaaa
     9061 tatattttgt tgatgattta gagctttctc cattagcttg tgcttatgca atggcaagag
     9121 gagcagatag aatgtcttct tatggcgatt ttatagcact ttcagatgta tgtgataagg
     9181 agactgcaac tattatagca agagaggttt ctgatggtat aatagctcca ggatatacag
     9241 atgaagccct tgaaatatta aaaggaaagc gtaaaggtaa ctataatatt gtaaaaatag
     9301 atgaaaatta tactcctgaa cctattgaaa caaaggatgt ttatggaatt acttttgaac
     9361 aaggtagaaa tgaaatatta ataaatgaag acctattaaa agatattcca actgataata
     9421 aaatttttac agatagtgca aaacgtgatt taattatagc tcttattaca ttaaaatata
     9481 cacaatcaaa ttctgtttgt tatgctaaag atggacaagt aataggggta ggtgcaggtc
     9541 aacaatcacg tattcattgt actagacttg caggtaacaa agctgatact tggtatcttc
     9601 gtcaacatcc taaagtatta aacttaaaat ttaaaaagga tataggtcgt ccagatagag
     9661 ataacactat tgatgtatat ctttctgatg attatatgga tgtgcttgct gatggcatat
     9721 ggcaaaactt ttttgaagaa aaaccagagc ctctaactgg agaaggaaaa agagcatggc
     9781 ttaaaacttt aacaggagta gcacttggtt cagatgcatt tttcccattt ggagataata
     9841 tagaacgtgc aaaaagaagt ggggtaagtt ttatagcaca accaggtggt tctattcgtg
     9901 atgataatgt tatattaact tgtaataagt ataacatagt catggcattt actaaaaata
     9961 gattattcca tcattaaaat actgaaagta taaagataat atagattaag taaaaatcta
    10021 caacaagttt tgttgtagat ttttttattt gctgtataat tttaaataga actttatttt
    10081 tagatattca atttggaggt tgatatggct ttaaatataa gaaactatag cctattatgt
    10141 aatcaaggaa cagctaaatt taatgaagat gtagtcggta taaatccatt tggggcatgg
    10201 gttttagatg gtgctacagg acttaatgga aaaaatttag tgtcaactga aagtgacgca
    10261 aggtggtatg ttcagtggtg gaataggtat ttatataaaa atataaataa agaagaatca
    10321 ttaaagagta ttattaatac tggaataata gaagtaaaaa aagagtatga actaagatta
    10381 aatgggttta aggttgaaaa attagattta ccatctagtt ctatagttgt tgtaaaattt
    10441 catgaaaata aagtagagta ttttttatta ggagattgtg ctttgtttat taaaaatgga
    10501 gattccaaag ttataaaaga taggagtatt tgtaaatttg ataatactgt atttgatgaa
    10561 atgagtaaac tttctaactt ggaagagatg gcttttgatg agattagaac tagtgttatg
    10621 gatacaatag taagcaatag attgaagaaa aatacaaaag agggctattg gatattggat
    10681 tttgatgagg aagctgtgga aaattctata tatggataca ttgaaataaa aaaagatttt
    10741 caattgatgc taacgagtga tggttttaca agtgcatgtg atagatataa tctaataaaa
    10801 gaagaagaat tgatacgtat agcagaaaaa tttggtatag gatatataca taataaagta
    10861 agggattttg aagataatga ctataaagcg gttaaagtgc caagatttaa agtaaaagat
    10921 gattcatctt gtttatattt agatatatat aatgattaga ctgtataaaa ctatttaatt
    10981 ttagaaagga ggcaagtgaa aagtgaaaat tcttcatata ataacacaaa aaccaaatag
    11041 tacaggtagt ggtatatatt tgtctggtat gataaaaggg ttcgaaaaaa tcggtcataa
    11101 gcaagctgta attgcaggta tagatgtaaa tgatgatgta aattgttttc caagtgaagt
    11161 aagtttttat ccagtaaagt ataattgtgg ggagttaaat tttcctgttg taggtatgag
    11221 tgattctatg ccatatgaaa gtacaagata taaagattta aatatagaca tgataaatag
    11281 attgaaatat caatttaaag taaatataga taaggctatg aatgatttta aaccagaact
    11341 tataatatgt catcatttgt acctcttaac tgcatttgtt agggaaatgg taaaagatat
    11401 aaaggttatg tctatttgtc atggaacttg tttaagacag ttaaatacca ttgatttaga
    11461 gaaagaatac ataatagcaa atataaggaa attagattta atatttgctc ttcatgaaaa
    11521 tcagaaatat gatattataa agacttttgg agtaagtgaa agtaaagtag ttgtaattgg
    11581 tagtggttat aatgatgata tattttataa taaaaattat aaaataaagg atgataaaat
    11641 aaaaatagta tttgcaggta agatatgtaa atcaaaggga ctgattccat ttataaaagc
    11701 tatatctaaa ttaaaatata gtaaagattt aatagaagtc aattttgcag gtactggaag
    11761 cgatatcgaa tcatataatg agatagttaa actggcaagt aagtctccat ttaaaatgaa
    11821 tttcctagga aagttagaac agagagattt agctgagcta ttcaacaggt cacaaatatt
    11881 tgtgttacca tcattttatg agggattacc agttgtagtt ttagaagctt tatcttgtgg
    11941 cacagatgta atcactactg atattcttgg tgttaaagag tggattggta gtgagattaa
    12001 taattctgga aagatagaat atgtttctct accttttatg gagaaagaag gtattccaaa
    12061 agatgaagag ttatatgatt ttgaaaataa tttgtataat gcaatagatt ctaagataca
    12121 atctttattg aataactcaa ataagaaaac atctgtagat atgtcaaaaa aaacgtggga
    12181 tggcttagca tatagaataa atgaagttat tttaatggat gaattgtgtt tagtctaaaa
    12241 ttatttaaat tataaaaaag tgtaactatt tttacactat ttttaaatga ttttatatta
    12301 tactaatact aaatatattt gttttaggga ggtaatttat gaggattagt aaaaaggtat
    12361 tggcgttagg tatttcagca ttactgttaa gtgttagttt tccaacatca ataaacgcac
    12421 tagataagat tgaaaatatt caaggagtag ataaatatga aacagctggg cttatttcag
    12481 ataagcaaga ttttacaaca gcagttctta tcaatgcaga ctctacaatg gtagatggga
    12541 ttgctgcaag tggtcttgca ggagtaaata atgcagctat tttattaact aataaggatg
    12601 atataccaga agcaacactt caaagactga atagagttac taagatatat gtaataggtg
    12661 gagaaaattc tataagtaaa gatgtagaaa aaatgttact tatgagaaga atgcaagtta
    12721 taagaattga tggtgtagat agagtagata caagttataa aattgcaggt gaaatagaaa
    12781 aaattaaaaa cagtgataag atgtttttag taaatggatt taaggatgaa gctgatgcag
    12841 taagtgttgc atctgttgct tatagagatg gagctcctat aatattgaca aaagatatac
    12901 cttcagcaga agaagacaca gacttagacc catggtttgg agtttctcct gttccagtat
    12961 atgctattgg aggagagtct acattaagtg attacatagt gagcagatat agagcaacta
    13021 gaattggtgg tgttgataga tatcaaacta ataaaaatgt tattgaaaag ttctataatg
    13081 gagcaaaaga gttctatata acaagtggag atgatttagt ttatgcttta gttgcatcac
    13141 cacttgcaaa aaatgctcct gttgttttag tttcaaataa aagtgataag agtatattaa
    13201 gtggagctag taaagtaaca gcaataggta taagtgataa gagtattata gagcaatgtt
    13261 tggatgctgt aaagaagtaa tggagtgtta ttagtaaaat gaatatatac attgaaaatt
    13321 agtagattgt tcattttata atggtataaa atatatggga agaggctatc aaatggagat
    13381 aagcctcttt ttcattgtta aaattatgaa aagatacttt attttatata aaaaagtatc
    13441 ttatgaacta aatgtctatt ttgagacact atctactttt cttatattat attatttttt
    13501 atatggaata aatttcataa gtattatctt cctttaattt ttctaaccaa accatctcta
    13561 aaatatctag ttcttctaaa tagtctttct ctgttttgtt atcatcatgt tttataagtt
    13621 ctaaaacttc aaatgtaaat gctttttcac catatttttt ccattcatta tttaattctt
    13681 ttatcataca tgagccagca ccaagctgaa atttgagaga gttaaatttg ctttttatgt
    13741 cattactttt tccaaggtaa atctttttag taatatcatt ttttattatg aatacacctg
    13801 tgtcaaattt catttctttg tataattgtt ttaattcacg ttttctatcc atgattttag
    13861 atacctctct ttataattga tatgtaatat gtaag
//
LOCUS       DAMJYR010000100        13883 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.103, whole genome shotgun sequence.
ACCESSION   DAMJYR010000100 DAMJYR010000000
VERSION     DAMJYR010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13883)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13883)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13883
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.103"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            187..744
                     /gene="efp"
                     /locus_tag="PC471_002799"
     CDS             187..744
                     /gene="efp"
                     /locus_tag="PC471_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HDF2789911.1"
                     /translation="MVSAGDFRKGVTFEKDGQPCLVVDFQHVKPGKGAAFVRTKYKNL
                     KTGAIREESFNPSEKFPKAVIDTRQMQYLYNDGELYYFMDQENFEQIPLNYEQVEDAI
                     KFLKENEVATIRFYQGQPFQVEAPNFAELEVTDTEPGIKGDTASNVTKAATVETGAVV
                     QVPLFINTGDKVKIDTRTGEYLSRV"
     gene            836..1291
                     /locus_tag="PC471_002800"
     CDS             836..1291
                     /locus_tag="PC471_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HDF2789912.1"
                     /translation="MKHLYEEVAYLKGLAEGLEISAESKEGKMIHKIVDALEVFADAI
                     VTLDEEQEELQDFVESIDEDLADLEEDIADMEEDLYEEDDDEDDEDFSYIEMECPNCG
                     ELVEIDEDLLYDDEVDVVCPDCKAVILSSEDDCDDDCTCGGCSSCDDRE"
     gene            1454..2164
                     /locus_tag="PC471_002801"
     CDS             1454..2164
                     /locus_tag="PC471_002801"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HDF2789913.1"
                     /translation="MKISKKLLDNIQRFENVINYKFKNKEYILEALTHSSYSNENKKY
                     NFNERLEFLGDSVLGIVISDYLFNEEANLPEGELTKLRANIVCEDSLSEVANDINLGI
                     HMLLGRGEEATGGRHRTSILADAFEAVIAAIYLDGGFESARQFILHHMENIIYDSRKG
                     NIFRDYKTHLQEVLQGNGENNIWYRLIEEKGPDHNKRFVMEVGINDDVLGIGEGKSKK
                     EAEQLAAKIALKKKLWEK"
     gene            2218..3288
                     /locus_tag="PC471_002802"
     CDS             2218..3288
                     /locus_tag="PC471_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HDF2789914.1"
                     /translation="MKKRIIPIFVPHKGCPHDCIFCNQKKITGVSTDVTSEDARNIIE
                     ECLETIDKDADVEIAFFGGSFTAIDIDIQKSLLSVAKEYVEKGLVKDIRMSTRPDCID
                     EDILSMLKEYKTSIIELGVQSLDEKVLLDSIRGHQSEIVYKSSKMIKNSGIKLGLQMM
                     IGLPADTEEKCIFTAKKFVELKPDCVRVYPTLVVKDTGLEKLMEQNKYNPFTLEESVN
                     IVKKVLVLFYVNNINVIRVGLQATDDIQIGKAVLAGPYHPAFRELVEADMIKDYLEFV
                     ILQNKNIKQMLVKSNKKNISKIIGNKKTNVKYMEEKFGVLLKTQESDLDINQLEIVLD
                     GKSLISANMRDIHRKLYDIYDL"
     gene            3316..6870
                     /gene="smc"
                     /locus_tag="PC471_002803"
     CDS             3316..6870
                     /gene="smc"
                     /locus_tag="PC471_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HDF2789915.1"
                     /translation="MYLKRLELKGFKSFPVKTDIIFKEGITAIVGPNGSGKSNISDAV
                     RWVLGEQSIKSLRGDKLEDVIFAGTDTKKPMNYCEVALTIDNSENQLELDFTEVTIRR
                     RAYRNGESEFFLNNKSCRLKDIKEVFLDTGIGKDGYSIIEQGKVDEILSNNPLSRRKV
                     FDEACGISKYRYKKQEAERNLSNTKENLERIDDVYIEIENQLKPLFNQQTKAKKYLEI
                     SEKLKTLEVNSFIREIEGIEKELSEVNEHRKVIEKELNEKEEQKNVVEKKQEDINKEV
                     EVLQDVIEKSVDYINSIKGVISKKESQINLIKERIRNFTNEISRKNLEIKDIKEKLNE
                     NKQYIKELESNKLSGSEELSTLQENIKVLEGSKDKQKIKLESLNNEIELLKESIIDIL
                     NKKQEFSNKLSTLNANKENMNIRDENINSEITELNKNIEIKSSELDTINKEFNMQNEN
                     LKNVNNRHKELSINLQDSISEHNKLEDEIQKSKYNLNGYNSKLNVYIDMENHYEGFNR
                     GVKEVLKNKNLKGVHGALGQIINVPEKYEKSIEAALGAYMQNIITDNEFSAKFAINYL
                     KQNNLGRVTFLPLNIIKSNKISLGNLKANTKFIGIASDLITFDEKYRNIIENILGRTI
                     LINNIDEGIKFAKETGHRFKIVTLDGEILNPGGSLTGGSLKTNGNILSRKRYINEYTE
                     KISNIKNEISHLELKRESLDKDVKNIKNEIDSHESKIKDLEKSIIIKSTSIKNVESEI
                     ESLKGSITKLENEKNDLNSNLNYTLEKSDDVRKDMEELDDLYNKNKEKIDALNEEIKR
                     YNDLYDKEKSEFDELNLSLVKKTEVYNSIVRDIKRISGENCELEEKNKQLEESLNYEE
                     HEIIKLQDSILTEEKEKENLTKQLGDSNRNLETRKIAKDDLKNSFDEINKELKTIDRQ
                     HIELKESLFKVGGRLERLKTSQDTYINKLFEQYDMTLVQALEIKDEDLDIDRKFLESL
                     KREIRSLGNINIDSIKEYEEIKERYDFYSEQKQDLEESMEEIEKLIHTLEENMKSEFE
                     IKFEEISKNFKYVYKRLFGGGCGELTILDKENLLESDILITAQPPGKKMKNLNLLSGG
                     EKALTAISILFAILITKPTPFCILDEIEAPLDDANIFRFGEFLKDLSKETQFISVTHR
                     RGTMEAADYIYGVTMQEKAISKVISLKLKEAQEITDII"
     gene            6897..8177
                     /gene="ftsY"
                     /locus_tag="PC471_002804"
     CDS             6897..8177
                     /gene="ftsY"
                     /locus_tag="PC471_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HDF2789916.1"
                     /translation="MLKKLFGFGKDKEKEIEKKDAEEEIEVEDSVDNLENLEETIFSG
                     LEEEVIDKVEDIEEKNEESVNNEGTEEIENFEKIEVEMDSNGKEVENISNNDSQEEII
                     EELENYDEIESKEIEDKEDKKVNLFERLKQGLTKAKQGITDRIDEVLKSYTKIDEELL
                     EDLEEILITADVGVNTTMDIIERLRDKIKQKGITEPIKVREELKSIVEDILTNENSTL
                     DIEPAPCIILMVGVNGVGKTTTIGKLANRYKKDGKKVLLAAADTFRAAATEQLEIWAN
                     RTNVDIIKHQEGADPGAVVFDAIKAAKARKTDVLICDTAGRLHNKANLMNELGKVFKI
                     VDREFPEAKREVLLVVDATTGQNAVVQAKTFKEVADITGIVLTKLDGTAKGGVVLAVK
                     SEVDVPVKLIGVGESVEDLQDFNAKSFSDALFGN"
     gene            8280..8648
                     /locus_tag="PC471_002805"
     CDS             8280..8648
                     /locus_tag="PC471_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HDF2789917.1"
                     /translation="MNIEKMVEIGLLFEQYKELLTDKQKEIVALYYEEDYSLGEISEN
                     LNVSRQGVYDTLKRSEKILRDYEEKLHLVSKIQEQEKNIKIIKDKIIDIKEDLLHNRD
                     CANLIPKLENIEDVCREMIK"
     gene            8645..9997
                     /gene="ffh"
                     /locus_tag="PC471_002806"
     CDS             8645..9997
                     /gene="ffh"
                     /locus_tag="PC471_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HDF2789918.1"
                     /translation="MIFEGLSDKLQGAFSKLKSKGKLTEVDVKNAMREVKLALLEADV
                     NFKVVKDFIKKVQERCVGQEVMQSLTPAQHVIKIVNEELTSLMGDVQSKVMISSKPPT
                     ILMMVGLQGAGKTTTAGKLGGYFKKQGKKPLLVACDIYRPAAIKQLQVVGEKLDIPVF
                     NMGDKENPINIAKAGLSHAIKNANDLVIIDTAGRLHIDEVLMDELKSIKSEVKPHEIL
                     LVVDSMTGQDAVNVAESFNEALGVDGVVLTKLDGDTRGGAALSIRAVTQKPIKFMGMG
                     EKLDDIEPFHPDRMASRILGMGDVLSLIEKAQENIDLEKAKELEQKIKKQELDFEDFL
                     QQMEQIQNMGPLDKILGMIPGMGNVKDQLGDVDLNGKEMKRTKAIIQSMTTYERRNPG
                     VLNASRKKRIAKGSGTSVQDVNRLIKQLNEMKKMMKMFAGSQKSMKKRGGLGGLPFFK
                     "
     gene            10035..10307
                     /gene="rpsP"
                     /locus_tag="PC471_002807"
     CDS             10035..10307
                     /gene="rpsP"
                     /locus_tag="PC471_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000268750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HDF2789919.1"
                     /translation="MAVKIRLKRMGANKKPFYRIVVADSRAPRDGKFIEEIGYYNPIS
                     EPKQVRINDEKAIKWLATGAQPTEVVKKLLVKNGVIEKFEASKQAK"
     gene            10372..10599
                     /locus_tag="PC471_002808"
     CDS             10372..10599
                     /locus_tag="PC471_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HDF2789920.1"
                     /translation="MKELVVDIAKALVDNPDSVVVEEFEDNDGIVLKLTVAQEDMGKV
                     IGKQGRIAKAIRTVVRSVANRENIKVSLEIV"
     gene            10707..11222
                     /gene="rimM"
                     /locus_tag="PC471_002809"
     CDS             10707..11222
                     /gene="rimM"
                     /locus_tag="PC471_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA processing protein RimM"
                     /protein_id="HDF2789921.1"
                     /translation="MKEKLTHFKVGQIVNTQGLKGEVRVYPLTDDIERFDELKDFYLG
                     KDLNNKLAVEKVRYKGSMVIMKIKGIDSIEKAEKLKNKFMYVAREDSRELEEGEFFIA
                     DMIGMEVLTVDGKYVGILEDVLQYSANDVYVVKGEEDKEFMIPAIKKFVPTIDIDERK
                     MIIDPIKGMID"
     gene            11231..11926
                     /gene="trmD"
                     /locus_tag="PC471_002810"
     CDS             11231..11926
                     /gene="trmD"
                     /locus_tag="PC471_002810"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000686892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HDF2789922.1"
                     /translation="MRFHIMTLFPEIFNSYMDESIMKRAVEKGIIEVHIYNIRDFSNN
                     KHKKVDDYPFGGGAGMVMTPQPIYDTYKHIITTHNINNPSVIYLTPKGKVYNQSMAKQ
                     MSLKEDIILLCGHYEGIDERIIDLIVTDEISIGDYVLTGGELPALIMIDSISRLIPGV
                     LNQEESFEEESFKDNLLEYPHYTRPRDFEGLKVPEVLLSGNHKKIDEWRREESIRITK
                     ERRFDLYKKSNEK"
     gene            12111..12461
                     /gene="rplS"
                     /locus_tag="PC471_002811"
     CDS             12111..12461
                     /gene="rplS"
                     /locus_tag="PC471_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HDF2789923.1"
                     /translation="MNEMLRAIEQEQLKNEVPNFGPGDTVKVHVRIIEGKRERIQVFE
                     GVVLKRQGGGARETFTVRKMSFNVGVERTFPVHSPKIEKIEVTRKGKVRRAKLNYLRG
                     RVGKAAKIKEARNK"
     gene            12782..13678
                     /gene="ylqF"
                     /locus_tag="PC471_002812"
     CDS             12782..13678
                     /gene="ylqF"
                     /locus_tag="PC471_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HDF2789924.1"
                     /translation="MSNEYEEYLMNDNLHINWYPGHMKKTKELVKNNLKLIDVVIELL
                     DARIPFSSKNPDIDRLVGDKPRVVVLNKSDMADRDKLNQWIEYYKKINIKAIPVDTIK
                     GVGINKIIEECKNVTREKMSSLKDKGRKERAIRIMIVGVPNVGKSSLINKLTGRKSTQ
                     TGDKPGVTKGKQWVRLKGNLELLDTPGILWPKFEDQEVALNLAFSRAIKDEILDTETL
                     ALRLIEKLMKIEPEKLKARYKLDCLGETPIETMDMIGHKRGFITGKKELDYTRIATTV
                     LNEFRDGKIGNITLEVPENVKR"
BASE COUNT         6019 a         1286 c         2660 g         3918 t
ORIGIN      
        1 taaaaatata gaataagtaa tttgccaaat acttgtttat attgtataac ttatgatatt
       61 atgatattag taaaaatttg taatcctgac ttgacagaat tcaagtaaga gatataattt
      121 taaaggcata gtaatgtaaa atttactaaa aaataagaaa tttattttaa tatggaggaa
      181 caaaaaatgg tatcagcagg tgattttaga aaaggtgtta catttgaaaa agatggacaa
      241 ccatgtttag tagttgattt tcaacacgtt aagccaggta aaggagctgc tttcgttaga
      301 actaaataca aaaacttaaa aacaggagct ataagagaag aaagtttcaa tccaagtgaa
      361 aaattcccta aagctgttat agatacaaga caaatgcaat atctatacaa tgatggtgag
      421 ttatattatt ttatggatca agaaaatttt gaacaaatac cattaaacta tgagcaagtt
      481 gaagatgcaa ttaagttctt aaaagaaaat gaagttgcta caataagatt ttaccaagga
      541 caaccattcc aagtggaagc gccaaacttt gcagagctag aagttacaga tacagagcca
      601 ggtataaaag gtgatacagc aagtaatgta actaaagcag ctacagttga aactggagca
      661 gttgttcaag ttccattatt tataaatact ggagataaag taaagataga tactagaaca
      721 ggtgaatatt tatcaagagt ataaaaattt atgtatataa aagcttatat gtcttaataa
      781 tataagatat atgagctttt agtttatata taaaaaagaa agcgaggggt gttacatgaa
      841 acatttatat gaagaagttg catacttaaa aggcctagca gaaggactag aaataagtgc
      901 tgaaagtaaa gaagggaaaa tgatacataa aatagttgat gcattagaag tatttgcaga
      961 tgctattgta acattggatg aagaacaaga agaacttcaa gattttgttg aatctataga
     1021 tgaagattta gcagatttag aagaagatat tgctgacatg gaagaagatc tttatgaaga
     1081 agatgacgat gaggatgatg aggacttcag ctatatagaa atggaatgcc caaattgtgg
     1141 agagttagta gaaatagacg aagatttgtt atatgatgat gaagtagatg ttgtttgtcc
     1201 tgattgtaag gctgtgatat tatcatcaga agatgattgt gatgatgatt gtacatgtgg
     1261 tggttgttca agttgtgatg atagagagta aaaattcata agaattaaaa ctgcctatga
     1321 tgcctatgag atattttgct tcataggtag ttttattttt atgaggaagg gtattatgta
     1381 ttttggtttt gcaatacttg gacaaaaata gtacactaga atttatagaa taggattttg
     1441 gagggatgta aatatgaaaa taagtaaaaa attactagat aatatacaaa gatttgaaaa
     1501 tgttataaat tataaattta aaaataaaga atatatacta gaagctctta ctcatagttc
     1561 atattctaat gaaaataaaa aatataattt taacgaaaga ttggaatttt taggggattc
     1621 ggttttaggc atagtgataa gtgattattt atttaatgaa gaagctaatc tgcccgaagg
     1681 ggaactaact aaacttagag caaatatagt ttgtgaagat tcattaagtg aagtagctaa
     1741 tgatataaat ttaggtattc acatgttatt aggtagagga gaagaagcaa caggaggaag
     1801 acataggact tcaatattag cagacgcatt tgaagctgta atagctgcta tatatcttga
     1861 tggaggtttt gaaagtgcaa gacagtttat acttcatcat atggaaaata taatttatga
     1921 ttcaagaaaa ggaaatatat ttagagacta caaaacgcat cttcaagagg tgttacaagg
     1981 gaatggagaa aataatatct ggtacaggct tatagaagaa aaaggaccag accataataa
     2041 aagatttgta atggaagttg gaattaatga cgatgtactt ggtataggag aaggcaagag
     2101 taaaaaagaa gctgaacagt tagctgctaa aatagcttta aagaaaaaat tatgggaaaa
     2161 ataaatataa tatgtaaaaa tataaaaaga aatatttata ttagaaggat gttgtagatg
     2221 aaaaagagaa ttatacctat atttgttcct cacaaaggat gtcctcatga ttgcatattt
     2281 tgtaatcaaa aaaaaataac aggtgtgagt actgatgtta ctagtgaaga tgcaagaaat
     2341 ataattgagg aatgtttaga gactatagat aaagatgcag atgtagaaat agcatttttt
     2401 ggtggtagct ttactgctat agatattgat atacaaaaaa gtttattatc agttgctaaa
     2461 gaatatgtag aaaaaggtct tgtaaaagat attagaatgt ctacaagacc agattgtatt
     2521 gatgaagata tattaagtat gttaaaagaa tataagacaa gtataattga actaggagta
     2581 caatccttag atgaaaaagt attattagat agtataagag gtcatcaatc tgaaattgta
     2641 tacaagagtt caaagatgat aaaaaatagt ggaatcaaac ttggattaca gatgatgatt
     2701 ggtcttccag ctgacacaga agaaaagtgt atatttacag caaaaaaatt tgtagagcta
     2761 aaaccagatt gtgttagagt ctatccaact ttggtagtaa aagatacagg tcttgaaaag
     2821 ttaatggaac aaaataaata taatccattt actcttgaag agagtgtaaa tatagtaaaa
     2881 aaagttcttg tactgtttta tgttaataat atcaatgtaa taagagttgg gcttcaagct
     2941 actgatgata tacaaattgg aaaagctgtt ttagcaggac catatcatcc tgcatttaga
     3001 gagttggtag aagcagatat gataaaagat tatttagaat ttgttatttt gcaaaataaa
     3061 aatataaagc aaatgttggt caaatctaat aaaaagaata tttctaaaat cataggaaat
     3121 aaaaaaacaa atgttaaata tatggaagaa aaatttggtg tgcttttaaa aacacaagaa
     3181 tcagatttag atataaatca attagaaatt gtgttagatg ggaaaagttt aattagtgct
     3241 aatatgagag acattcatag aaaattgtat gacatttatg acctttagtt ttaataatga
     3301 agggagagaa aagatttgta tttaaaaaga ctagagttaa agggatttaa atcctttcca
     3361 gtaaaaacag atattatttt taaagaagga ataacagcca tagtaggacc aaatgggagt
     3421 ggaaaaagta atatatctga tgctgtaaga tgggtattag gagaacagag tattaagagt
     3481 cttagaggtg ataaactcga agatgtaata tttgcaggta cagatacaaa aaaacctatg
     3541 aattattgtg aagtggctct tactattgat aattctgaaa atcaattgga attagatttt
     3601 acagaagtaa ctataagaag aagggcttat agaaatggtg aaagtgaatt ttttctaaat
     3661 aataaaagtt gtagattaaa agatataaaa gaagtctttt tagatactgg tattggaaaa
     3721 gatggttatt caatcataga acaaggaaaa gtagatgaaa tattaagtaa taatccttta
     3781 agcagaagaa aagtttttga tgaagcttgt ggcatatcaa aatatagata taaaaaacag
     3841 gaagcagaaa ggaatttaag taatacaaag gaaaatttag aaagaataga tgatgtatat
     3901 atagaaattg aaaatcaatt aaagcctctt tttaatcaac agacaaaggc aaaaaagtac
     3961 ttggaaataa gtgaaaaatt aaagacactt gaagtaaata gctttataag agaaattgaa
     4021 ggaatagaaa aagaattaag tgaggttaat gaacatcgta aggttattga aaaggagctc
     4081 aatgaaaaag aagaacaaaa aaatgttgta gagaaaaagc aagaagatat taataaagag
     4141 gtagaggtat tacaagatgt aattgaaaaa tctgtagatt atataaattc aattaaagga
     4201 gttatatcaa aaaaagagtc ccaaataaat ttaattaagg aaagaataag aaattttacg
     4261 aatgaaatta gtagaaaaaa tttagaaata aaggatataa aagagaagct aaatgaaaat
     4321 aagcaataca taaaagaatt agaaagcaat aaattatcag gtagtgaaga actatctacc
     4381 ttgcaggaaa atataaaagt attggaagga agtaaagata aacaaaaaat taagttagaa
     4441 tccctaaata atgagataga attattgaaa gaaagtataa ttgatatatt aaataagaaa
     4501 caagagttta gtaataagct gtctacttta aacgcaaata aagaaaatat gaatatcaga
     4561 gatgaaaata taaattcaga gataacagag ttaaacaaaa atattgaaat aaaatcatct
     4621 gaattagata ctataaataa agaatttaat atgcaaaatg aaaatttaaa gaatgtaaat
     4681 aatagacata aagaattatc aattaatttg caggattcaa ttagtgaaca caataaacta
     4741 gaagatgaaa tacagaaaag taaatataat ttaaatggat ataattcaaa gttaaatgtc
     4801 tatatagata tggaaaatca ttatgaagga tttaatagag gagtaaaaga agtactaaaa
     4861 aataaaaatt taaaaggagt tcatggagca ttaggacaga ttataaatgt acctgaaaag
     4921 tatgagaaat ctatagaagc agctcttggt gcttatatgc aaaatataat aactgataat
     4981 gagtttagtg ctaagtttgc aataaactat ctaaaacaga ataatttagg tagagtaaca
     5041 tttcttccat taaatataat taaatcaaat aaaataagtc taggaaattt aaaagcaaat
     5101 actaagttca taggaatagc aagtgattta attacatttg atgaaaagta tagaaatata
     5161 attgaaaata tacttggaag aacaatactt ataaataata tagatgaggg cataaaattt
     5221 gcaaaagaaa ctggtcatag gtttaaaata gtaactttag atggtgaaat cttaaatcca
     5281 ggaggttctt tgactggggg aagtctaaaa actaatggga atatactatc gagaaaaaga
     5341 tacattaatg aatatacaga aaagataagt aatatcaaga atgaaatttc acatctagaa
     5401 ttaaaaagag aatctttaga caaagatgtg aaaaatatca aaaatgaaat tgattcacat
     5461 gaaagtaaaa taaaagattt agaaaaaagt ataataataa aaagtacaag tataaagaat
     5521 gttgagtctg aaattgaatc gttaaaaggt agtattacta aattagaaaa tgagaagaat
     5581 gatttaaatt ctaacctaaa ttatacactt gaaaaaagtg atgatgttag aaaagatatg
     5641 gaagagttag atgatttata caataaaaat aaagaaaaaa ttgatgcatt gaatgaggaa
     5701 ataaaaagat acaatgattt atatgataaa gaaaaatctg aatttgatga attgaactta
     5761 agtttagtga agaaaacaga agtttacaat agtatagtta gagatataaa aagaatatct
     5821 ggtgaaaatt gtgagctaga agaaaaaaat aaacaattag aagagtcatt aaattatgaa
     5881 gaacatgaaa taattaaatt acaagattct atactcactg aagaaaaaga aaaagaaaat
     5941 ttaacaaaac aactagggga tagtaataga aatcttgaaa ctagaaaaat agcaaaagat
     6001 gatttaaaaa atagttttga tgaaattaat aaagaattaa aaactataga tagacagcat
     6061 atagaactta aagaaagttt atttaaagtt ggtggaagat tagaaagatt aaaaactagt
     6121 caagacacat atataaataa gttgtttgag caatatgata tgaccttagt acaagcattg
     6181 gaaattaaag atgaagattt ggatatagat agaaaattct tagaaagtct aaagagagaa
     6241 ataagaagtc ttggaaatat aaatatagac tctataaagg aatatgagga aataaaagaa
     6301 agatacgatt tttatagcga acaaaagcaa gatttagaag agtctatgga agagatagaa
     6361 aaacttatac atactttaga agaaaatatg aaatctgaat ttgaaattaa gtttgaagaa
     6421 ataagtaaaa attttaaata tgtatataag agattatttg gtggaggttg tggagaatta
     6481 actattttag ataaagaaaa tttattagaa agtgatatac taataacagc tcaaccccca
     6541 ggtaaaaaaa tgaaaaattt aaacctatta tctggtggag agaaagcttt aacggcaata
     6601 agtatattat ttgctatatt gattacaaag ccaacaccat tttgtatatt agatgagatt
     6661 gaagcgccac ttgatgacgc aaatatattt agatttggag aatttttgaa ggatttatct
     6721 aaagagactc aatttatatc agtaactcat aggagaggaa caatggaagc tgctgattat
     6781 atctacggag ttacaatgca agaaaaagcc atttcaaaag ttattagttt aaaattaaaa
     6841 gaagctcagg aaataacaga tattatataa cttagattga cataggagga ataacagtgt
     6901 taaaaaaatt atttggtttt ggaaaagaca aggaaaagga aatagaaaaa aaagatgctg
     6961 aagaagaaat agaagttgaa gacagtgttg ataatttaga aaacttggaa gaaactattt
     7021 tttcaggact tgaagaagaa gtgatagata aagtggaaga tattgaagaa aaaaatgagg
     7081 aaagtgtcaa taatgaaggc actgaagaaa tcgaaaactt tgaaaaaata gaagttgaga
     7141 tggatagtaa tggaaaagaa gtagaaaata tttcaaataa tgattctcaa gaagagataa
     7201 tcgaagaatt agaaaattat gatgaaattg agtctaaaga gatagaagat aaagaagata
     7261 agaaagttaa cttgtttgaa agactaaaac aaggattgac aaaagcaaag caaggaataa
     7321 ctgatagaat agatgaagtt ttaaaatcat atacaaaaat agatgaagaa ttacttgaag
     7381 atttagagga aattttaata acagcagatg ttggtgtaaa tacaactatg gatataattg
     7441 aaagactaag agataaaata aagcaaaaag gtattactga accaataaag gtaagagaag
     7501 aacttaaatc tatagttgaa gatattctta caaatgaaaa ctctacacta gatatagaac
     7561 cagcaccatg tataatatta atggtgggtg taaatggggt cggtaagact actacaatag
     7621 gtaaactagc aaatagatac aaaaaagatg gtaaaaaagt tttattagca gcagcagata
     7681 catttagagc agcagcgaca gagcaattag aaatttgggc aaatagaacc aatgtagaca
     7741 taataaaaca tcaagaggga gcagaccctg gagctgtagt atttgatgct ataaaagctg
     7801 caaaagctag aaaaacagat gtattaatat gtgatacagc aggaagatta cacaataagg
     7861 ctaatcttat gaatgaactt ggaaaagtat ttaaaatagt agatagagaa tttccagaag
     7921 caaaacgtga agtacttctt gtggtggatg ctacaacagg tcaaaatgca gttgttcaag
     7981 caaagacatt taaagaagtt gcagatataa caggtatagt acttacaaaa cttgatggaa
     8041 ctgcaaaagg tggagtagtc cttgcagtaa aatcagaagt agatgtacct gtaaaactta
     8101 ttggagttgg agaaagtgta gaagacctac aagatttcaa tgcaaaatca ttttcagatg
     8161 cattatttgg taactaatgt tcttttaaaa ataatggttg acaagagtgt tgttatgata
     8221 taaaatacaa tagtgtaaag taaataacct ttacagtata ctgctaggaa gtgtaagata
     8281 tgaatataga aaaaatggtc gaaattggat tgttatttga acagtataaa gaattattaa
     8341 ctgacaagca gaaagaaata gttgctctat actatgaaga agattattct ttaggtgaaa
     8401 ttagtgaaaa tctgaatgta tcaagacaag gtgtttatga taccttaaag cgttcagaaa
     8461 aaatactaag agattatgaa gaaaagttac acttagtatc aaagattcaa gagcaggaaa
     8521 aaaatattaa gattataaaa gataaaatta ttgacattaa agaagattta ttgcacaata
     8581 gagattgtgc taatttaatc cctaagctag aaaatataga agatgtatgt agggagatga
     8641 taaaatgata tttgaaggat tgtctgataa actacaaggg gcatttagta agttaaaatc
     8701 taaaggaaaa cttacagaag tagatgtaaa aaatgctatg agagaagtta aacttgctct
     8761 tcttgaggca gatgttaact ttaaagtagt taaagatttc ataaaaaaag ttcaagagag
     8821 atgtgttggg caagaagtta tgcaaagctt aactccagct cagcatgtaa tcaaaattgt
     8881 aaatgaagag cttacaagtt taatgggaga tgtacaaagt aaagtaatga tttcctcaaa
     8941 gcctcctaca attttaatga tggttggatt gcaaggtgca ggtaagacta ctacagcagg
     9001 aaaacttgga ggatatttta aaaagcaagg taaaaaacct ctattagtag cttgtgatat
     9061 ttatagacca gcagctataa agcaacttca agttgttgga gaaaaattgg atatacctgt
     9121 attcaatatg ggagataaag aaaatccaat aaatattgcg aaagcaggtt taagccatgc
     9181 gattaaaaat gctaatgatt tagttattat agatacagct ggtagacttc atatagatga
     9241 agtacttatg gatgagttaa agtctattaa atcagaagtt aagccacatg agatactttt
     9301 ggttgtagat tctatgacag gtcaagatgc tgtaaatgtt gcagaaagct tcaatgaggc
     9361 acttggtgtt gatggagtag tattaactaa attggatgga gatactagag gtggtgctgc
     9421 gctttcaata agagcagtta cacaaaagcc tataaaattt atggggatgg gagaaaagct
     9481 agatgatata gaacctttcc acccagatag aatggcatca agaatattag gtatgggaga
     9541 tgtcttaagt ttaatagaaa aagcacaaga aaatatagac ttagagaaag ctaaagaatt
     9601 agagcaaaaa attaaaaaac aagagcttga ttttgaagat ttcttacaac aaatggaaca
     9661 aatacaaaat atgggacctc ttgacaagat acttggtatg atacctggta tgggaaatgt
     9721 aaaagaccaa cttggagatg tagatttgaa tggcaaagaa atgaagagaa caaaagccat
     9781 aattcagtca atgactacat atgagagaag aaacccagga gtattaaatg cttcaagaaa
     9841 gaaaagaata gctaaaggca gtggaactag tgtacaagat gtaaatagac ttataaaaca
     9901 acttaatgaa atgaaaaaaa tgatgaagat gtttgctggt tcacaaaaga gtatgaagaa
     9961 aaggggcggc ttaggcggtt tacctttttt taaataaaat ataaaaaagt aaaaattatt
    10021 cggaggtgac tgtaatggca gttaaaataa gattaaaaag aatgggagct aacaaaaaac
    10081 ctttctacag aatagtagta gctgattcaa gagctccaag agatggaaaa tttatagaag
    10141 aaataggata ctataatcca atttctgaac ctaaacaagt aagaataaat gatgaaaaag
    10201 ctataaaatg gttagctact ggtgctcagc caacagaagt agttaaaaaa ttacttgtaa
    10261 aaaacggagt aatagaaaaa ttcgaagctt ctaaacaagc aaaataattt tttcttggca
    10321 attagtttaa gtaagggctc attgctaagg ttactttagg agatgaaata tatgaaagag
    10381 ctagttgtag atatagctaa ggctctagtt gataatcctg actcagttgt tgttgaggaa
    10441 tttgaagata atgatggtat cgtcttaaag cttacagttg ctcaagagga tatgggtaag
    10501 gttattggga agcagggaag aatagctaaa gctataagaa ctgtcgtaag atctgttgca
    10561 aatagagaaa atataaaagt ttctcttgag atagtataaa ataggaggat taggtataat
    10621 gcctagtcct ttttatataa atgcgtagtt taagatttta tgatgatatg accacataag
    10681 attaaatttt ttaagaaggt gataacatga aagaaaaatt aactcatttt aaggtaggtc
    10741 aaatagtaaa tacacaaggt ttaaaaggtg aagtaagagt ttatccgctt actgatgata
    10801 tagagaggtt tgatgaatta aaagatttct atctgggaaa agatttaaat aataaattgg
    10861 ctgttgaaaa agtaagatac aaaggttcta tggttataat gaagataaaa ggcatagaca
    10921 gcatagagaa agcagaaaaa ctaaaaaata aatttatgta tgtagctaga gaagatagca
    10981 gagagcttga agaaggcgaa ttttttatag cagatatgat tggaatggaa gttttaactg
    11041 tagatggaaa atatgtaggg atactagaag atgttcttca atactcagca aatgatgtct
    11101 atgtagtaaa aggtgaggaa gataaagaat ttatgatacc agctataaaa aaatttgtac
    11161 ctacaataga tattgatgaa agaaaaatga ttatagaccc tattaaggga atgatagatt
    11221 aggtggtaat atgagatttc atataatgac actttttcca gaaatattta attcgtacat
    11281 ggatgaaagt ataatgaaga gagcagttga aaaaggtata attgaggttc atatatataa
    11341 tataagggat ttttctaata ataaacataa aaaagtagat gattatccat ttggaggagg
    11401 agcggggatg gtaatgactc ctcaacctat atatgacaca tataagcata taataacaac
    11461 tcataatata aataacccaa gtgtaattta tcttactcca aaaggtaagg tatacaatca
    11521 atctatggca aagcagatgt ctttaaaaga agatataata ctactatgtg gtcattatga
    11581 aggaatagat gagagaatca ttgatttaat agttactgat gaaatttcaa ttggagatta
    11641 tgtattaact gggggagagc tcccagcact tataatgata gattctatat cgagattaat
    11701 accaggtgta ttaaaccaag aagaatcatt tgaagaagaa tcatttaaag acaacttatt
    11761 ggaatatcct cattacacta gacctagaga ttttgaagga ttaaaagtac cagaagtgct
    11821 tttatcaggg aatcataaga agatagatga gtggagaaga gaagaatcta taaggataac
    11881 caaagaaaga cgatttgatt tatataaaaa atctaatgaa aaataaaaat ataatgaaaa
    11941 attaatatat atgtatacaa taaagtacct ataaatggaa ttaaaaacat aaaagtgctt
    12001 gtaataacta ttgaagtata gtataatata aaatgttgaa aatacgggcg ttcctctgtt
    12061 tacactggtc gggtagttaa gaacgtctat gatatctagg aggaagaaag atgaatgaaa
    12121 tgctaagagc aattgagcaa gaacaattaa aaaatgaagt tcctaatttt ggacctgggg
    12181 acacagttaa agtacacgtt agaattatag aaggaaaaag agaaagaata caagtattcg
    12241 aaggtgtagt attaaagaga caaggtggag gagcaagaga aactttcaca gttagaaaaa
    12301 tgtctttcaa tgttggtgta gaaagaactt tcccagttca ctctccaaaa atagaaaaaa
    12361 tagaagttac tagaaaaggt aaagtaagaa gagctaaact aaactactta agaggtagag
    12421 taggtaaagc tgctaagata aaagaagcta gaaataaata attaaaaact ttacttataa
    12481 atggggggct gatttatcag tcctctttct attttaatga aaattacagc aaataattat
    12541 actagaaata aatagaaata tttgttgaaa atatacatta tttatctatg aatttatagc
    12601 ttttaaaatg aaaaataaaa ttggtaaata ttgcattata ttttgtaaca atattaaaaa
    12661 taagagaaca gtattatgta aagtatgacg ttaaaaagat gacaatatta tagtttgtta
    12721 agatagattt tttaagtgga attaggatat aataatataa tattaataag gagtgataca
    12781 tgtgtcaaat gaatacgaag agtatcttat gaatgacaat ctacatataa actggtatcc
    12841 aggtcatatg aaaaaaacta aggagttagt aaaaaataac ttaaaattga ttgatgtagt
    12901 aattgaactt ttggatgcta gaatcccgtt tagtagtaaa aatccagaca tagacaggct
    12961 tgtaggagat aagccaagag ttgtcgtact taataagagt gatatggcag atagggataa
    13021 attgaatcaa tggattgaat actataagaa aattaatatc aaagctatac cagtagatac
    13081 aataaaaggc gttggtataa ataagataat cgaagaatgt aaaaatgtaa ctagagaaaa
    13141 aatgagttct ttaaaagaca aagggagaaa agaaagagct atacgaataa tgatagttgg
    13201 agttccaaac gttggtaaat cgtctcttat aaacaaatta acaggtagaa aaagcactca
    13261 aactggagat aagccaggag taacgaaagg gaaacaatgg gttagattaa agggaaatct
    13321 tgaactttta gatacacctg gaatactttg gcctaagttt gaagaccaag aagttgcatt
    13381 aaatttagca tttagtagag ctataaagga tgagatttta gatactgaaa ctttggcttt
    13441 aagacttata gagaaactta tgaaaataga acctgagaag ttgaaagcca gatataagtt
    13501 agattgtcta ggagaaactc caattgaaac tatggatatg attggccata aaagagggtt
    13561 tataacaggt aaaaaagaac tagattatac acgcatagcc acaactgtat taaatgaatt
    13621 tagagatgga aaaataggaa atataacatt ggaagttcca gaaaacgtta aaagatagat
    13681 tttaaaacaa aatcaaaaat ttaatgttaa aaatttaaaa aagcctttta gcaaaggctt
    13741 ttttattttt ttgaaatata gacaaatatc tccatataaa agtataaatt aggttagaaa
    13801 acctttaaaa atattaattt aagacaaata tacctaatat tttgaaaatt aaaatgagtg
    13861 tgattattta aaattttagc ata
//
LOCUS       DAMJYR010000101        13519 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.104, whole genome shotgun sequence.
ACCESSION   DAMJYR010000101 DAMJYR010000000
VERSION     DAMJYR010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13519)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13519)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13519
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.104"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            325..1755
                     /locus_tag="PC471_002813"
     CDS             325..1755
                     /locus_tag="PC471_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDF2789925.1"
                     /translation="MSSQESKPKGNLISNFRDDMRDPKKRAIYTYSSSINLAYNFIGY
                     FVSTVTNIFLTDVAKLGVIMAGGMQIIQSFIKIAVAPITGTVFDKQPFKKGKYYPWLR
                     YAPAALALTYGIFFIVPLMKISPALLLPLMTALLLIASCAQQVVFTIIYAIYPVVAKT
                     PRDRVIGSTTTNIFKEVGKFLVGFSYPLLLVLFADVLGGEGMGYLGTYLFFAAGCLLV
                     FWFSSTEIIKSGAEEEVLQKEEKPKIKASEMAKALFTNVPLMMAFCLEFLICIRSIGL
                     GPLAPYYFKYVIEDERGLAVFLSIMPLVSVAFMFFAPVFIKICREQKLASIISFSICA
                     LCHLLVAWTPWGKTTLGVTMLLAIGGGFSNVVSIINLNFFAGSCDYGHWKSGKDLPGL
                     SMSLYPVAIQVGVLLATTIRTVLMNSMGYQADMVVTEAVKSGFINMISYSMAIPLIIA
                     VVIAILYPVSDKKLNQIREELNQRNA"
     gene            1858..2961
                     /locus_tag="PC471_002814"
     CDS             1858..2961
                     /locus_tag="PC471_002814"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HDF2789926.1"
                     /translation="MIEKLLRNTVKELHQYVPGEPIEKVKEKYGVKEIIKLASNENPL
                     GPSPKAIEAMIEMLKQGQLYPEPEANELRRKLAEKLDLKPENFIVANGADNVITLIGE
                     AFINRGDEVIYCNPTFPSYRTATIKNEGIPVEVPLTEDYKYDLQGILDKITDKTKLIC
                     VCNPNNPTGTIVDDKELEEFLKKVPENIITILDEAYIEFLTVPNYVDGLKYVRENYNV
                     IVTRTFSKIYGLAGLRVGYGIAKDEIIRTLFTVKEPFSANRVAISGATAALDDEEFIK
                     ETYELNRVGMAYFKEEFTKMGFDVVDSQSNFLYVDMKTDIPKLFEDLKKRGFVIRPSA
                     THARVSIGTMEENRKFVAVLKEILNIKGEPSIG"
     gene            2976..3212
                     /locus_tag="PC471_002815"
     CDS             2976..3212
                     /locus_tag="PC471_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HDF2789927.1"
                     /translation="MKRGVDMNLKINAERCKSCEYCVISCKKGALKISSKINKEGYAH
                     VEVDESKCILCGICYQVCPDNVFEIIKEEACQEA"
     gene            3229..4278
                     /gene="vorB"
                     /locus_tag="PC471_002816"
     CDS             3229..4278
                     /gene="vorB"
                     /locus_tag="PC471_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HDF2789928.1"
                     /translation="MKKMWKGNHAIAEAALRGGCEFYAGYPITPQTEVMEFLSHRMSE
                     LGRTFIQSENEMAAIYMVYGAYASGMRSMTSSSGPGISLKQEGISYLCANHYPCVIVN
                     VQRWGPGLGSLDSAQTDYLRDTRGGGNGDYHLIVYAPNSIQETVDLMYNSFDVAEKYR
                     VPVEILTEAALGQMMEPVEFPEFKKREGDLGWTYDGSNRDHAKVADNQKPTFCMEKRM
                     RITENEQQWEDYQVEDAEYVFVAFGIPSRTTMNAVRRLREQGEKVGIIRPITVWPFPY
                     KAFEKVSKDVKGFISVESTDTGQLVEDVALASKKVCKENVPVYGLFSGNHIPKTLQVM
                     DTYSKIKSGEIKEVF"
     gene            4280..5020
                     /locus_tag="PC471_002817"
     CDS             4280..5020
                     /locus_tag="PC471_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate oxidoreductase"
                     /protein_id="HDF2789929.1"
                     /translation="MATEKLTPSVVSIPNKFCPGCGHGIVNRIIAEVIEEHGYEKNHV
                     LTLGVGCVCNMNFSWNGDKMQTAHGRASSTAIGVKVALPDTLVMTYQGDGDAYVIGLS
                     ETLNTAYRNHNVTVFVINNNNFAMTGGQMSWTTMPGQVTTTSVNGRDIKTTGSPLKVP
                     EMVANFFDVAYVARGSVHSPKEIINLKKYIKNAIEAQLNGEGYSLVEILAPCPTNWGV
                     SLEKSIKWMEEEIVPYYALGEFKQRDGE"
     gene            5023..5562
                     /locus_tag="PC471_002818"
     CDS             5023..5562
                     /locus_tag="PC471_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HDF2789930.1"
                     /translation="MEKELVFAGFGGQGVLTLGLIVAESALELGKQVTWMPAYGPTMR
                     GGKAYSVVKFSDESIGGPDMEEIDVLVAMNKPSLDYINLVKEGGTVVINTSAIDENVT
                     LREDISVVKINCQELAQKVNNPKAANIVVLGALIAKTGLFEKELALKTMCDFFEEKGK
                     GKFNVQNEAAFMEGYNNAI"
     gene            5686..7785
                     /locus_tag="PC471_002819"
     CDS             5686..7785
                     /locus_tag="PC471_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HDF2789931.1"
                     /translation="MSLEKLIRPKSIAIVGVTDKLGFGRSAALSIVKSKETDRVYYVN
                     PKREELFGRKCYKTIQEVPEVVDCVVVCTPRNVVPSVLKDSGELGTKAAVVYASGFAE
                     EGTEEGTDLENQLIEISNTYDMKILGPNCMGLLNCIDKVNLWAGGSKWDLDTKKPGIG
                     IVGQSGFITAEIVSSDYFNISYGFSTGNGNIVTLEDAVDFLVDDDYASVIAIYLEGLK
                     NPQKFIDALKRAAQKRKPVIILKSGRSEKGAISAASHTGNLAGSSKAFESIFEKYGVI
                     SVENLEQFMCLAQAFSVLDGNLPANSNFAAINFSGGENTICADLAEENGVELAEISSE
                     TKEEMEKYLPGFATPKNPLDATTALFHEKDMIVGLLHTFEKDDSVGFTMVGANIRDEE
                     NEMHETLCKAVSEAREQGLKKPVFAVPTLEGNRYLDYRTRLEDNQVPIMSSVGTTFTC
                     FNKMAKFIDYDYSKRTLEFKAVKKRESNNVVALSELDSKIEMKKYGVPVPGQGNAKSI
                     DELDEILKYIKYPVVLKINSSEILHKSDVGGVKIGIKNRDEAVDAYNEILTNVKKAKP
                     DANIDGILVQEMVESGIEIIIGITNDDQFGPMLLVGLGGVFVEVFKDTTLYPLPINHD
                     EAIMMLKKLKSFKLLNGYRGSEPCDIDALADMMVKLGKYAYENKDEVKEIDLNPVFVY
                     PKGKGVCAVDALIVKYK"
     gene            complement(8052..8567)
                     /locus_tag="PC471_002820"
     CDS             complement(8052..8567)
                     /locus_tag="PC471_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin"
                     /protein_id="HDF2789932.1"
                     /translation="MISAKMEKLLNEQINHELYSAYLYLSMSSYLEAEGLKGFSNWFY
                     VQYKEETDHAMFFYKYLHNVGGKVQLDAIPMPDSDFTSAMDILERTLAHEKKVTALIN
                     NLAAVANSESDFRTSQFLLWFISEQAEEETNCEDNIKRVKLAGEGGLFFVDQEFANRT
                     YAAPTNPPVTI"
     gene            complement(8659..9870)
                     /locus_tag="PC471_002821"
     CDS             complement(8659..9870)
                     /locus_tag="PC471_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HDF2789933.1"
                     /translation="MSNKQGSNIKDVKIKNKLIILKLLSTNTPMSRVDLAKSTGLTKM
                     TLSNLVTELINENMIREIESSTLNAHSSGRKPILLDISNISPCICGILIRRNICKVII
                     SDLKGNIVKQLSETYPKFLSNDVLKDIIKRMFTKIMKINKRDIIGIGISSIGPVDDIN
                     GTILNPPDFGNVSNLNIVDFIKEMSDLPIFLINDANSGALAEKMYGLGKNISNYIYLH
                     IMNGIGAGLVLENKLHTGNLGQSGEIGHTSINFSGPLCSCGNNGCLDYYTSVSNLIKR
                     IESLSHIYPNSPLINCKDFSLVKLIDEANNKDSLAMFLLDEFCTYVSYALVNTLTLID
                     CSSIIIGYDFNIPGTFIENTLLKKLTASASFSKYKKISVRHSNFGANAPLIGSIAIVS
                     YNFFNGSINFY"
     gene            10546..11988
                     /locus_tag="PC471_002822"
     CDS             10546..11988
                     /locus_tag="PC471_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HDF2789934.1"
                     /translation="MRTIRRVLALGLTLAIFLINVPNVDALTSDTIKGNNIYETAGLI
                     ADKKSYDTAIMVNMDNSIADGLSASGLAGAVDAPILLAQKNKIPNETKQRLKNVKKIY
                     IIGKELSISKSVETELKNTGAQVTRLGGDDRIKTSYSVAKEVNGIKKVDEVILTNAYK
                     GEADTISAAPVSVRDIAPIVLTDGKSVPFSTSNVKTYAVGGSISMSTSLVNKTNAKRL
                     GGSDRYDTNKKVIKEFYPDASEFYLSDGYDLVNALTGSTIAKENPIVLVSESSDKSIL
                     AGADKITRLGSISDSVYNKCVSAAQNNGDSSTKGESPMKNETSILGQPTASLEACLKW
                     AKSKKANDLFIELIPILYDTAVQEGVNPVLAVAQSAKETGFCNFGGVLDASFKNPCGL
                     KTSVGGSDTDKNAHSRFDTWEEGILAQIQHLCLYAGQDGYPLSNPVDPRHEKSLFGKA
                     KTVESLSNNWAGGQYGQDLVRMMGEIEATK"
     gene            12030..12383
                     /locus_tag="PC471_002823"
     CDS             12030..12383
                     /locus_tag="PC471_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2789935.1"
                     /translation="MKKSNIFSIIFPTVIMVLMFLTFATDIFNIPDIQSKSIFVVGMV
                     LIFPISFLIQGIICVLNKTNWILSLIVSLVTYIVLMYIFLNDSAYIYVLLYTAFYMIG
                     HITAKLYYKIKTFKN"
     gene            12574..13395
                     /locus_tag="PC471_002824"
     CDS             12574..13395
                     /locus_tag="PC471_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase HisJ family protein"
                     /protein_id="HDF2789936.1"
                     /translation="MNLIDNHIHTNFSSDGKDSMEDTIKKAISIGVRYLTFTDHLEHD
                     EDRGFCINYNDYVPVFNKFKEKYKKDIELLLGVEVGYRKHLKNEIEEIINSNPFDFVL
                     CSTHTIDNVPVPSKAYFKGLSKEDAYYKYFNSILETNREFGDYNIYGHLDYISRYGIY
                     SDNRVIYNDFKDVIDEVLKSIINNGSGIELNTSGYRYGLNAIHPNEDILKRYKELGGF
                     IVTVGSDSHRVEDICKDFDVAYDMLKYLDFKYVSLFKERETYFVNIEKVKSNNIA"
BASE COUNT         5154 a         1581 c         2433 g         4351 t
ORIGIN      
        1 cttgaataaa attataaaaa attaaaatca ctagaaatat gataaaataa tttaaaatac
       61 gcatttcaat gacatttcaa aacaagaaaa taataaaaaa caaacctaaa ttaagtctaa
      121 atagcttttt agttaatttt agtagtaaaa aataaaactt aaaaagtgtt tgacaattat
      181 gaattttgag aatataatta aattaaaatt atacacatat aaaaatatac tcctgaatca
      241 agaatgagat atttaagatt caaaatatat attattatac gtgaataata aaaagtaagt
      301 aaattttata agggaggggt agatgtgagt agtcaagaaa gcaaaccaaa aggaaatctt
      361 atatcaaatt ttagagatga tatgagagac cctaagaaaa gagcaatata cacatattca
      421 agttctataa atttagcgta caattttata ggttactttg tatcaactgt tacaaatatt
      481 tttttaactg atgtggcaaa acttggtgta attatggcag gtggtatgca aattatacaa
      541 tctttcataa aaattgctgt agctcctata acaggtacag tatttgacaa acaaccattc
      601 aaaaaaggaa agtattatcc atggcttaga tatgcaccag cagcgttagc attaacatat
      661 ggaatatttt tcatagttcc attaatgaaa ataagtccag cattactttt acctttgatg
      721 acagctttat tattaatagc atcttgtgct cagcaagtag ttttcactat aatttatgct
      781 atttaccctg tagttgctaa gacacctaga gaccgagtta taggctcaac tactacgaat
      841 atatttaaag aagttggtaa attcttagta ggtttttcat atccgttatt attagtttta
      901 tttgcagatg tattaggtgg agaaggaatg ggttatttag gtacctactt attctttgca
      961 gcaggatgcc ttttagtatt ttggttttct tcaacagaga taataaaatc tggagcagaa
     1021 gaagaagttc ttcaaaaaga agagaagcca aagattaaag caagtgaaat ggcaaaagct
     1081 ttattcacta atgtaccact tatgatggca ttttgtttag aatttcttat atgtataaga
     1141 tctataggat taggaccttt ggctccatat tattttaaat atgtaataga agatgaacgt
     1201 ggattagcag tatttttatc aatcatgcct ttggtaagtg tagcattcat gtttttcgca
     1261 ccagtgttta taaaaatctg tagagagcaa aaactagctt caataatttc tttttctata
     1321 tgtgcactat gccacttatt ggttgcatgg actccttggg ggaaaactac tttaggagta
     1381 actatgttgt tggcaattgg tggaggtttt tcaaatgtag tatcaataat aaatcttaat
     1441 ttctttgccg gttcttgtga ttatgggcat tggaaatcag gaaaagactt acctggctta
     1501 agtatgtcac tatatccagt tgcaatacaa gtaggagtat tacttgcaac tactataaga
     1561 acagttttaa tgaactcaat gggatatcaa gctgatatgg tagtcacaga agcagttaaa
     1621 tcaggtttta taaatatgat ttcttattct atggcaatac ctttaataat agctgttgtt
     1681 atagcaatac tatatccagt aagtgacaag aaattaaacc aaataagaga agagttaaat
     1741 caaagaaatg cttagttaaa agaaagttga gtagatggtt aatttaagct attaaagtaa
     1801 gtagacgcaa tagcgtttat ataaatgaaa atacatatac agagggggct tttaaaaatg
     1861 atagaaaaat tattgagaaa taccgtaaaa gaattacatc aatatgttcc aggtgaacca
     1921 atagaaaaag ttaaggaaaa atatggtgta aaagaaataa taaaattagc ttcaaatgaa
     1981 aatcctctag gaccatcacc aaaagcaata gaagctatga ttgaaatgtt aaaacaaggt
     2041 caactttatc cagagccaga agctaacgaa cttagaagaa aattagctga aaagctagat
     2101 ttaaaaccag aaaattttat tgttgctaat ggtgcagata acgttatcac tctaataggt
     2161 gaggctttta taaatagagg agatgaagtt atatattgta acccaacatt tccatcatat
     2221 agaactgcaa ctataaaaaa tgaagggata ccagtagaag tacctttaac agaagattat
     2281 aagtatgatt tacaaggtat attagataag attacagata aaacaaaatt aatatgtgta
     2341 tgtaacccta ataatccaac aggaacaatt gtggatgata aagagttaga agaattttta
     2401 aagaaagtcc ctgaaaatat tataactata ttggatgaag catatataga atttttaaca
     2461 gttcctaatt atgtggatgg acttaagtat gtaagggaaa attataacgt tatagtaaca
     2521 agaactttct ctaaaatata tggtcttgca gggcttagag taggatatgg tattgctaaa
     2581 gatgagataa taagaacttt atttacagta aaagagcctt tctctgcaaa tcgtgttgct
     2641 ataagtggag caacagcagc acttgatgat gaagagttta taaaagaaac atatgaatta
     2701 aatagagttg gaatggctta ctttaaagaa gagtttacaa aaatgggatt tgatgttgta
     2761 gattcacaat caaatttcct ttatgtagat atgaaaacag atatacctaa gttatttgaa
     2821 gacttgaaaa aaagagggtt tgtaataaga ccaagtgcta cacatgcacg tgtaagtatt
     2881 ggaactatgg aagaaaatag gaaatttgta gcagttttaa aggaaatatt aaatattaaa
     2941 ggagagccaa gtattggata atagatagaa atattatgaa acggggtgta gatatgaact
     3001 taaaaataaa cgcagagaga tgtaaaagtt gtgaatactg cgttatatct tgtaaaaaag
     3061 gagcattaaa aatatcaagt aagataaata aagaaggtta tgctcatgta gaagttgatg
     3121 agagtaaatg catactttgt ggaatttgct atcaggtgtg tccagataat gtttttgaaa
     3181 taattaaaga agaagcatgt caagaggctt agaaggagtt gggataatat gaaaaagatg
     3241 tggaagggga accacgccat tgcagaagca gccctaagag gaggttgtga attttatgca
     3301 ggatatccaa ttacaccaca aacagaggta atggaatttt tatcacatag aatgtctgaa
     3361 cttggaagaa cttttataca gtccgaaaat gagatggctg ctatatatat ggtatatgga
     3421 gcatatgcaa gtggaatgag gtctatgacg agttcttcag gaccaggaat atctttaaaa
     3481 caagaaggga tttcttactt atgtgcaaat cattaccctt gtgtaatcgt aaatgtacag
     3541 agatgggggc ctggtcttgg ctcattggac tcagcacaga cagactatct aagagatact
     3601 agagggggag gaaatggaga ttatcatttg atagtttatg caccaaacag tattcaagag
     3661 actgtagatt tgatgtataa ctcatttgat gtagcggaga agtacagagt gcctgtcgaa
     3721 attttgacag aagcagcttt aggtcaaatg atggaaccag tagaatttcc agagtttaag
     3781 aagagagaag gagatttagg ttggacttat gatggaagta atagagacca tgcaaaggtt
     3841 gcagataacc aaaagccaac tttttgtatg gagaaacgta tgagaataac tgaaaatgaa
     3901 caacaatggg aagattatca agtagaagat gcagaatatg tgtttgtagc gtttggtata
     3961 ccatcaagaa caactatgaa tgctgttaga agacttagag aacaaggtga aaaagttgga
     4021 ataataagac caataacagt atggcctttc ccatataaag catttgaaaa ggtaagtaaa
     4081 gatgtaaaag gatttatctc agtagaatca acagacacag gtcaattagt agaagatgtt
     4141 gctttagcct caaagaaagt atgtaaagaa aatgtacctg tgtatggttt atttagtgga
     4201 aatcatatac caaaaacatt gcaagtaatg gacacttata gcaaaataaa atctggcgaa
     4261 atcaaggagg tgttctaata tggctacaga aaaattaaca cctagcgttg tttcaatacc
     4321 aaacaagttt tgcccaggtt gtggtcatgg aatagtaaat cgtataatag cagaggtaat
     4381 agaagaacat ggatatgaaa aaaatcatgt attaacatta ggagttggtt gtgtctgcaa
     4441 tatgaatttt agctggaatg gtgataagat gcaaactgca catggtagag cttcatctac
     4501 agctatagga gttaaagtag cacttccaga tacattagta atgacttacc aaggtgatgg
     4561 agatgcttat gttataggtc tttctgaaac tttaaataca gcatatagaa atcataatgt
     4621 gactgtattt gtaataaaca ataataattt tgcaatgact ggtggacaaa tgtcatggac
     4681 cactatgccg ggtcaagtca caacaacaag tgtaaatgga agagatataa agactactgg
     4741 tagtccactg aaagttcctg agatggtggc aaacttcttt gatgtagcat atgtagcaag
     4801 aggttcagtt catagcccaa aggaaataat taatctaaaa aaatatataa aaaatgcaat
     4861 agaagctcaa ttaaatggag agggatattc cttagttgaa atattagcac cttgcccaac
     4921 taactggggt gtatcactag agaaatctat caaatggatg gaagaagaaa tagtgccata
     4981 ttatgctctt ggcgaattta agcaaaggga tggtgaatag ttatggaaaa agagctagta
     5041 tttgcagggt ttggtggtca aggagtttta actcttggtc tgattgtagc tgagtcagca
     5101 cttgaattgg ggaaacaagt aacttggatg cctgcatatg gtccaactat gagaggtgga
     5161 aaagcctata gtgtagtaaa gttttctgat gagagtatag gtgggcctga tatggaagaa
     5221 atagatgttt tagtagcaat gaataaacca tctttagatt atataaattt agttaaagaa
     5281 ggtggaacag tagttattaa tacatctgca atagatgaga atgtaacatt aagagaagat
     5341 ataagtgtag ttaaaataaa ttgtcaggaa ttagctcaaa aagtaaacaa tccaaaagca
     5401 gcaaatatag tagtacttgg agcattaatt gctaaaactg ggttatttga aaaagaatta
     5461 gctttaaaaa ctatgtgtga tttctttgag gaaaaaggaa aaggaaagtt taatgttcaa
     5521 aatgaagcag cattcatgga aggctataat aacgccattt gataaattac ccttatctta
     5581 agtgatttat ctcaagattt aataatcttt agtgccaaga gaaacttcat cttgagataa
     5641 aattcactta aaatcacaac tataaacata taggagcgtg aatttatgag tttggaaaaa
     5701 cttattagac caaaaagtat tgctatagta ggagtgactg ataaacttgg ttttggaaga
     5761 agtgcagcac ttagtatagt aaaaagtaag gaaacagata gagtatatta tgttaatccc
     5821 aaaagagaag aattgtttgg gagaaaatgt tataagacga tacaagaagt tccagaagta
     5881 gtagactgtg tagtagtatg tacgcctaga aatgtagttc catctgtatt aaaagattct
     5941 ggagagttag gaacaaaggc agcagtggta tatgctagtg gatttgcaga ggaaggtaca
     6001 gaggaaggta cagatttaga aaatcaactt atagaaatat caaatacata cgatatgaaa
     6061 atattgggtc caaactgcat ggggttgcta aattgtatag ataaggttaa tctatgggca
     6121 ggaggttcta aatgggattt agatactaaa aaacctggta taggaattgt aggtcaaagt
     6181 ggatttataa ctgctgaaat tgtttcaagt gattatttta atatatctta tggtttttca
     6241 acaggaaatg gaaatatagt tacactagaa gatgcagtag atttccttgt agatgatgat
     6301 tatgcatcag taatagcaat ttaccttgaa ggtttaaaaa atcctcaaaa atttatagat
     6361 gctttaaaaa gggcagcaca aaaaagaaag ccagtaataa tattgaaatc aggaaggtca
     6421 gaaaaaggtg ctatttctgc tgcttctcat acaggaaatt tagctggttc gagtaaagct
     6481 tttgaaagta tttttgaaaa atatggagta atttctgtgg aaaatttaga acagtttatg
     6541 tgtttagcac aagcttttag tgttttagat ggtaatttac ctgcaaattc taattttgca
     6601 gcaataaact tctcaggagg agaaaatact atttgtgcag atttagcaga agaaaatggt
     6661 gtagaacttg cagaaatatc ttctgaaact aaagaagaaa tggaaaaata tcttccagga
     6721 tttgcaacac ctaaaaatcc actagatgct acaacagcat tattccatga aaaggatatg
     6781 atagtaggtt tattacatac ctttgaaaag gatgatagtg ttggatttac tatggtaggt
     6841 gcaaatataa gagatgaaga aaatgaaatg catgaaaccc tttgtaaagc tgtaagtgaa
     6901 gctagagaac aaggattgaa aaaacctgtg tttgcagttc ctacacttga aggaaataga
     6961 tatttagatt ataggactag attagaagat aatcaagtgc ctataatgtc atcagttgga
     7021 actacattta cttgttttaa taaaatggct aagtttatag attatgatta ttcaaaaaga
     7081 actttagaat ttaaagctgt taagaaaaga gagtcaaata atgtagtagc attatcagaa
     7141 ttagatagta agattgagat gaaaaaatat ggagtaccag ttccaggaca aggtaatgca
     7201 aaaagtattg atgaattaga tgaaatctta aaatatatta agtatcctgt tgttttaaaa
     7261 ataaattctt ctgaaatatt gcacaaatct gatgttggag gagttaaaat aggaattaag
     7321 aatagagatg aggcagtaga tgcatataat gagattttaa ctaatgtaaa aaaagcaaaa
     7381 ccagatgcta atattgatgg tatattagtt caagaaatgg tagaaagtgg tatagaaatt
     7441 attattggta taactaatga tgaccaattt ggaccaatgt tattagtcgg tttaggagga
     7501 gtatttgtag aggtatttaa agatactaca ttatatccat tacctataaa tcatgatgaa
     7561 gctattatga tgcttaaaaa actaaaatca tttaagcttt taaatggcta tagaggaagt
     7621 gagccatgtg atattgatgc tcttgcagat atgatggtca agttaggaaa atatgcttat
     7681 gaaaataagg atgaagtcaa ggaaatagac ttaaatccag tgtttgtata tcctaaagga
     7741 aaaggcgtat gtgcagtaga tgccttaata gttaaatata aatagtttat aagttttggt
     7801 attcaaccat aattaaagca ccaactgaaa atcacatttt gtaagttaga tagtgtgatt
     7861 ttcagtcacc ccaaatttaa tttaaaaata ttttaaaatg aaagtatagt tttaagtttc
     7921 attgtgaaat agcactgtat aaacaataaa atactacgta taaaacgaat aataaaaggt
     7981 gttttaatga taagaatcaa tcttgatttt cattaaaaca ccttctttat tatattaaaa
     8041 atttttaaac gttatattgt cacaggtggg tttgttggag ctgcatatgt acgattagca
     8101 aattcttggt caacaaagaa tagcccaccc tctccagcaa gtttaacacg ttttatattg
     8161 tcttcacaat ttgtttcttc ttctgcttgt tcagatataa accaaagtaa gaattgagaa
     8221 gttctaaagt ctgattcaga gtttgctaca gctgctaagt tgtttattaa agctgtaacc
     8281 tttttttcat gagctaacgt acgctcaaga atatccattg cagaagtaaa gtctgaatct
     8341 ggcattggga tagcatcaag ctgaaccttt cctcctacat tatgtaagta tttgtaaaaa
     8401 aacatagcgt ggtctgtctc ttctttatat tgaacataaa accaattaga aaatccttta
     8461 agtccttctg cttcaaggta agaagacata gaaagataaa gatacgcaga atataattca
     8521 tggttaatct gctcattaag taatttttcc attttagctg aaatcatttt tatacctcca
     8581 aattttaaat gctcttataa aatataataa tattaaatgg aaaaatattc aataaaagtg
     8641 tttaaatatt ttacatattt aataaaaatt tatactacca ttaaaaaaat tataacttac
     8701 aatggctata gagccaatta atggtgcatt tgctccaaaa tttgaatgcc ttacacttat
     8761 ttttttatat ttagaaaaac ttgctgaagc agtcaatttt tttaataatg tattttctat
     8821 aaatgtacca ggtatattaa agtcatagcc tattattata gaactacaat caatcaatgt
     8881 aagtgtatta actaatgcat atgatacgta tgtacaaaat tcatcaagca aaaacatggc
     8941 aagactatct ttattatttg cctcatctat taacttgact aaagaaaaat ctttacagtt
     9001 aattaatggg gagttaggat aaatatggct caatgattca attcttttaa ttagattact
     9061 gacacttgta taataatcta aacatccatt gttaccacaa ctgcacaatg gtccactaaa
     9121 attaattgat gtatgtccta tttctccact ttgtcctagg ttgccagtat gaagtttatt
     9181 ttctagaact agaccagctc caataccatt cattatgtgt aggtatatat aatttgatat
     9241 atttttacct aaaccataca ttttttctgc taatgcaccc gaattagcat catttatcaa
     9301 aaatattggc aaatcagaca tttcttttat aaaatctact atatttaggt ttgatacatt
     9361 tccaaaatct gggggattta gtatggtacc atttatatca tctactggac ctattgaaga
     9421 aataccaatt ccaattatat ctctcttgtt gattttcatt attttagtaa acattctttt
     9481 tattatatct tttaaaacat catttgatag aaatttaggg taagtttcag aaagttgttt
     9541 tacaatatta ccttttaaat ctgaaataat taccttacat atatttcttc ttattaggat
     9601 tccacaaata caaggcgata tattagaaat atctaataaa ataggtttac gacctgatga
     9661 atgtgcattt aaagttgatg actctatttc cctaatcata ttttcattaa taagttcagt
     9721 aactagattt gataaagtca ttttagtaag tcctgtagac ttagctaaat caactctgga
     9781 cataggtgta tttgtagaaa gtaatttcaa tattattaac ttatttttaa tttttacatc
     9841 tttgatgtta gaaccttgtt tgttactcat cataaatctc cttaaataaa gttaagtgtt
     9901 ttatttattt tcaagtatac tatcacctta taatagagta acattttttt acttgaaaat
     9961 atcattaatt agaatatatc acattatata tcataaaaca tataaaaaaa tattagaatt
    10021 aaattaaaag tgaaaagtag attgatatat aaatagtaaa tacttaatta aaaaaagata
    10081 atgatagcaa taattattgt taccaagaat ctactacttc aagtgttacc ttaggtattt
    10141 aagttgatag tggtttgcac gtaataaact tgatgtaata tatattctta ataatatcga
    10201 taaaagattt acttaaaaat aatatagatt atgtaaacat ataatctatt tattgtatac
    10261 aataaaattt cattttgtaa aacagtgaat taaatatgta atatagatat cgatttttat
    10321 tacaaattgt tactaaatat aggtacatta gaacaaaaat ctagtaaatt gtaactaaaa
    10381 tgtaaatttt tcctttaatc tatacaatat tatggaaact ttttgtaatt tatgatataa
    10441 taacaaatat ataattatta aattataata aatgactata gcgtagatgt aatactatgt
    10501 tatagtcatt tttgaattaa acttgatata tcaaggggga agaatatgag aacaattaga
    10561 agagttttag cattaggttt aacattagct atatttctaa taaatgtacc taatgtagat
    10621 gcattaacat cagatacaat taaaggtaac aatatatatg agacggctgg attaatagca
    10681 gataaaaaga gttatgatac agcaattatg gtaaatatgg ataattctat agcagatggt
    10741 ctttcagcaa gtggtcttgc aggtgctgtt gatgctccaa ttttgcttgc acaaaaaaat
    10801 aagataccaa atgaaacaaa acaaagatta aaaaatgtta aaaagatata cataataggt
    10861 aaagaattat caataagtaa gtcagtagaa actgaactaa aaaacacagg ggcacaggta
    10921 actagattgg gtggagatga cagaataaag actagttata gtgttgctaa agaggtaaat
    10981 ggtatcaaaa aagttgatga agtgatatta actaatgcat ataaaggaga agcagataca
    11041 ataagtgctg cacccgtatc agtaagagat atagctccta tagtacttac agatggaaag
    11101 agtgtgcctt tttcaacaag caatgtaaaa acatatgcag taggtgggag tatatcaatg
    11161 agcactagtt tagttaataa aactaatgct aaaagacttg gtggttcaga tagatatgat
    11221 acaaataaga aagttataaa agagttttat ccagatgcat cagaatttta tttgagtgat
    11281 ggatatgatt tagtaaatgc acttacaggt tctacaattg ctaaggaaaa tccaattgta
    11341 ttggtgtcag aaagtagtga taagtctata ttagcaggag cagataagat tactagatta
    11401 ggttcaataa gtgacagtgt gtataataag tgtgtttctg ctgcacaaaa taatggcgat
    11461 tcgtctacaa aaggtgagtc acctatgaaa aatgagacaa gcatattagg tcagccaaca
    11521 gctagtttag aagcgtgctt aaaatgggca aaatctaaaa aagctaatga tttatttata
    11581 gagttaatac caatattata tgatactgct gttcaggaag gtgttaatcc tgttttggca
    11641 gtagctcaat ctgcaaaaga aactggtttt tgtaattttg gtggagtatt agatgcatca
    11701 tttaagaatc cttgtggact taaaacttct gtaggtggtt ctgatactga taagaatgcc
    11761 cattcaagat ttgatacttg ggaagaagga atattagctc aaattcaaca tttatgctta
    11821 tatgcaggac aagatggtta cccactttca aatccagtag accctagaca tgaaaaatct
    11881 ttatttggta aggctaaaac agttgaaagt ttatctaata attgggctgg aggtcaatat
    11941 ggacaagatt tagtaagaat gatgggagaa attgaggcaa caaaataatt gaagattatc
    12001 aaatatccaa taaataagga ttgattatta tgaaaaagag taatattttt agtatcatat
    12061 ttcctacagt cataatggtg ctaatgtttt taacttttgc tacagatata tttaatattc
    12121 cagatattca atctaaaagt atttttgttg tgggaatggt tttgattttt ccaatttctt
    12181 ttttgataca agggataatt tgtgtattaa ataagactaa ttggatttta tctcttattg
    12241 tttctttagt aacttatata gtattaatgt atatattttt aaatgattct gcatatatat
    12301 atgttttact ttatacagct ttttatatga ttggacatat aacagctaaa ctctattaca
    12361 aaattaagac ttttaaaaat taaattgaag tatataaaag taacattagg agaagtatcc
    12421 ttaatgattt tctaaaatca gaaataggtg cttctctttt ttgtaaaaat aaaagtatgt
    12481 aaataaaatt taaccattat aattttcttt taacaaatgt atacaataaa cttgttgtat
    12541 aattaagata gataaaataa agggagggat aatatgaatt taatagataa tcatattcat
    12601 actaattttt caagtgatgg aaaggattcc atggaagata caataaaaaa agctataagt
    12661 attggtgtta gatatttgac ttttacagac catttagaac atgatgaaga tagaggattt
    12721 tgcataaatt ataatgacta tgttccagta tttaataagt ttaaagaaaa atataaaaag
    12781 gacatagagt tattactagg tgttgaagtg ggttacagaa aacatctaaa aaatgaaata
    12841 gaagaaataa taaattctaa tccttttgat tttgtattgt gctcaactca tacaattgac
    12901 aatgtacctg ttccatcaaa ggcgtatttt aaaggtctta gcaaagaaga tgcgtattat
    12961 aaatatttta atagcatact tgaaactaat cgtgaatttg gagattacaa tatatatggt
    13021 catttggatt atatatctag gtatggaata tattcagata atagagttat atacaatgac
    13081 tttaaagatg ttattgatga ggtcttaaag tcaataatca ataatgggag tggtatagag
    13141 ctaaatactt ctggttatag atatggacta aatgctatac atccaaatga agatattcta
    13201 aaaagatata aagaacttgg aggatttata gttactgtag gttctgattc tcatagggta
    13261 gaagatatat gcaaagattt tgatgtggcc tatgatatgc ttaagtatct tgactttaaa
    13321 tatgtatctt tatttaaaga aagagaaacc tattttgtaa atatagaaaa agttaaatct
    13381 aacaatatag cttagagtgt gttttgaagt aaattaatag atagttaaaa agttaatagt
    13441 tatagtattg tatcctctag aaaaatacta gaggttttta ttttgctaaa ataactaaat
    13501 agaattaaat ttaaatagt
//
LOCUS       DAMJYR010000102        13353 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.105, whole genome shotgun sequence.
ACCESSION   DAMJYR010000102 DAMJYR010000000
VERSION     DAMJYR010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13353)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13353)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13353
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.105"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            228..929
                     /locus_tag="PC471_002825"
     CDS             228..929
                     /locus_tag="PC471_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4253 domain-containing protein"
                     /protein_id="HDF2789937.1"
                     /translation="MSIEDLYDFECVPLNNEIDIDVLFEKMIEDGKEKEYTPIIVIDE
                     KVGLLRKNIDMITKEYGSLENYREYCLTKYNEIDVNQFFDFRKSEISELLKELIDLED
                     DFYDYKHFRPIMNIDIFHEYNKVYIAKIPTIKPYEVFAYIPIGGFNDCPRDEEHIAIA
                     KYWYEKHGIFPIAIGCDTVQYSVKNLTKDGKKFDELCIELVFYCEDIIIQGYETLKAL
                     KDVLKRSTIVLFWWD"
     gene            1200..2018
                     /gene="larE"
                     /locus_tag="PC471_002826"
     CDS             1200..2018
                     /gene="larE"
                     /locus_tag="PC471_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent sacrificial sulfur transferase
                     LarE"
                     /protein_id="HDF2789938.1"
                     /translation="MEVNFVKEKEKLDKLKKMLLELGSVVVAYSGGVDSNFLLKVAKD
                     TLGENVIAVTIHAMMHSSREIEEAKQYTQNFGVKHIILNIENFDLKEFKENGVDRCYH
                     CKKYIFSKIKEVAKEHNIKYIVDGTNIDDLGDYRPGLKALSELGVISPLKDSSLKKEE
                     IRSLSKTLGLNTFNKPSFACLASRIPYGVEITDENLRIVEKSEEYLSNLGFSQFRVRM
                     HGDIARIEVGQEELGKFFENNNFNKVDTKLKIFGFKYVTLDMSGYKMGSMNLNV"
     gene            2073..3221
                     /locus_tag="PC471_002827"
     CDS             2073..3221
                     /locus_tag="PC471_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HDF2789939.1"
                     /translation="MKNYTLISPCFFGMEKMLAREITNLGYEIIKTEDGRITYKTDEF
                     GIAKSNMWLRCAERVHLKIAEFEAKSFDELFENTKRINWSRYIPYGAQFPISKASSIK
                     SKLYSTPDVQAIVKKAIVESLKKSYLEDGLLKEDKEKYPIFVFIHKDKVTISIDTTGD
                     ALHKRGYREKANKAPIRETLAAGLIYLTPWKAGRVLVDPMCGSGTILIEAAMIGINMA
                     PGLNREFISEKWRTLDKKIWWDVRKDAFNKIDNESKFKIYGYDIDEESIDIARENAEI
                     AGVDEYIEFNVGDATQFKSEDEFGFIITNPPYGERLEDKDSVKQLYKELGYAFRKLKN
                     WSYYLITSYEDFEYEFGQKADKKRKLYNGMLKTNFFQYPGPKPPRNNK"
     gene            3357..4745
                     /locus_tag="PC471_002828"
     CDS             3357..4745
                     /locus_tag="PC471_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HDF2789940.1"
                     /translation="MKVLKLITVVALMMLFVTGCNIKTESPEQLAKIPDYDDTKKVLY
                     DGIDQLLKPDSSMILPSNTKEVGKINKVDLNSDGIDELVVFEQKEDLSNNVSQVGFVT
                     LSYNGEKYVLGDHFLENGESIEYANFYDLDSDGYKEVILLVKSKDKTNMHIYKVVDNE
                     ITKIYDLDASWLQNKEDFNDMKVKIGYIDGDDKLDILMLHLNNKTNEMFASVANFDGK
                     MKIKDSVKFENVKNLSELYITLGNVASSVGNSSAAIKGIVLDIPILKDNNYITQILYM
                     KDGKINKAFSDYDKTITKSYYIPVDDIDKDKIIEIPIVAGSTGNNKNTYSSKSSATIS
                     WYRWNGKEGADSSLIFTSQIYYNYKYNFKLFIPNNLFDKILVEQEFVGEKAVFKFYYF
                     DYSDRKNLFNIVVESKNKLEDRKNTGTQNSIVLQESDEYTFLLVVNNANEMDKLDMTI
                     DALKEYFSLIYG"
     gene            4774..5472
                     /locus_tag="PC471_002829"
     CDS             4774..5472
                     /locus_tag="PC471_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2789941.1"
                     /translation="MKEKILILEDEIGIRSFVSINLKREGYEIVEAGTGREAIEKMTT
                     EKDITIALLDVMLPDISGIEVCKFIRENFDQVGIIMLTAKAQEDDKIEGFISGADDYI
                     IKPFSIKELLVRVSALLRRVAKDDSSVKSSEIVSPPFILDIDKRKLFKNGKEIELTPT
                     EFSIVKYLISNAKQSLSRDQILDEVWGTNYLYDFKIVDVNIRRIRNKIEDDPSKPKYI
                     QTIWGYGYCFRKEE"
     gene            5475..6908
                     /locus_tag="PC471_002830"
     CDS             5475..6908
                     /locus_tag="PC471_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF2789942.1"
                     /translation="MLHFEGLRKKVIKNYFIIIIIMVTLFEGLFMFYIQNYYYDSVKQ
                     LLESEIKYADEYNAITMETTSFEKKVKNIFDKQPLTKNSEFGISIIDKDKNIILDQYG
                     FKSKEKANYEDVNNALKDIKTKNLTPYTYRIPDTGEHVMSISLPLKVNNIIEGVVRYT
                     VSLDAIDNAILKQATWLILAGIFILIIAILISLKFAETLIKPLRELKKFANELAVGNY
                     NIKLEKMKIVDDEIGDLAQTFEHMAHEIDKSEKLKEEFISSVSHELRTPLTSIKGWSE
                     TLGYESITREELDLGLGIIQDETERLIKLVEELLDFSRLSSDRIKLHVDIVDVEGLIV
                     GVVNQLKVKAAEKDISLLFEFENEFIENIQGDKNRLRQVLINLIQNSFKFTSQGGYIK
                     VVASQDEEITTISVEDNGSGIEKQNLNKVLDKFFQEDYNKAGSGLGLAISNEIVKLHG
                     GRMKIESEKNVGTKITFNIKNKFAKQA"
     gene            6930..7667
                     /gene="truA"
                     /locus_tag="PC471_002831"
     CDS             6930..7667
                     /gene="truA"
                     /locus_tag="PC471_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011010156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HDF2789943.1"
                     /translation="MRNIKMIVAYDGSRYKGYQKLGDNNMTIQEKLENVLSKMTNETV
                     EIIGSGRTDMGAHARGQVVNFRTNCMDSLDKIQKYLYEYLPEDIVVKTVEEVDERFHS
                     RYNVKSKTYMYKIDNNKYHSPFIRKYATHVSKKLDLDRMRKASEYLVGEHDFTSFASS
                     KSKKKSNVREIYSINIKEDDNVIEIYVEGNGFLYNMVRIIVGALIDVGLKRRAPQDIK
                     YMLESKDRCQSSDTAPAKGLCLWKVTY"
     misc_binding    7778..7985
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            8238..9437
                     /locus_tag="PC471_002832"
     CDS             8238..9437
                     /locus_tag="PC471_002832"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HDF2789944.1"
                     /translation="MKEKVVLAYSGGLDTSIIIPWLKENYEDIDVIAVCGNVGQEDKM
                     EDVYEKALQSGASKAYVDDISEEFVTETIFKAVKAEAKYEGKYLLGTSLARPIIAKKL
                     VEVAHKEGAKYICHGCTGKGNDQVRFEATIAALDPTIKVIAPWRIWDIKSREDAIDYA
                     EKHNIKVTATKAKIYSVDANLWHVSTEGGDIEHLENEHKKDVYKQCVDPEDACDVAEY
                     VEVYFEKGVPKKVNGEELSPVALIHKLNELGCKHGIGVIDIVENRLVGMKSRGIYETP
                     GGTILYEAHNILESATLDKDTLHFKQMVSYKYGELIYNGLWYCKLRESIDAFMEQTQD
                     NVTGTVKVKLYKGNIKPAGIFTENALYDEGISSFGNSELYDHKDAEGFINLFTLPLKI
                     RAMKAGK"
     gene            9684..10730
                     /locus_tag="PC471_002833"
     CDS             9684..10730
                     /locus_tag="PC471_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4872 domain-containing protein"
                     /protein_id="HDF2789945.1"
                     /translation="MRKIIEIKHHSHDYECMWNGIEDLYINKTGECLPNDFFFLLSGF
                     GSFCYMKTNKADLKRMVALGDGRTKQMYKFLAPIVGFEYKHHEFKKYEQALKKAKSEI
                     DDGYPVILGALDMYYLPYYKKLYHKEHIPFHYILMVGYDDEGIYLYDCGRTELLHLSY
                     DKLRESMNCNSPGLSKENTICTIRMNTSKNKYNIAREAIAIKRDMFLNPPKGFLGYKG
                     FEKFIKELPQWNTELDKDDYDKILTNMVMFFGTVPTIPNAIKGIKEPDTVQFKGGFDK
                     MSRMLNSIGEEYKNDSFIQVADIFDKTAIIIEKISNIIIDYLTQTCDDTGQLPLLFSE
                     VLEHMKTGYLILDV"
     gene            10838..12001
                     /locus_tag="PC471_002834"
     CDS             10838..12001
                     /locus_tag="PC471_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDF2789946.1"
                     /translation="MRTVKQVSDLTGISVRALHYYDEIGLLKPNKITDAGYRLYDDES
                     IKTLQQILFFKEIDIPLREVKEIMSSQYFDKVEALKNQKKLLILKRKRLDELIELINQ
                     TLRGEGNIDFKEFDMSEYFDVLEEFKREHRNKVIKIYGSVEKYNEYIERVKSNEEKIA
                     KMAIKQYGTIEKFAKAIKTNFSSDILNLGEKFDRYKNDCLKEKHPKLKELYRKLVEDL
                     NRNPSSTDLQEIAKEITDISKKDYEIFSMDTGDDNWYYMVQNYLVNPMWIEEVDKKYG
                     SGAGKFIGQVLKTYLRDRKPKINTLYEKLVEDLSRDCSSREVQSIVEEIDNEMKRSNE
                     FYKIDNGERYFDYMSELYLQDSNYIKVTDKNYGDGASKFIGEAFKIYFDNNNC"
     gene            complement(12090..12470)
                     /locus_tag="PC471_002835"
     CDS             complement(12090..12470)
                     /locus_tag="PC471_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4363 family protein"
                     /protein_id="HDF2789947.1"
                     /translation="MKSTTFVILWTILFVLFGFYVNNKLYDFTEGYKDNISVLEKSIE
                     NEDWEKAQKEADSISTSWNKEKNHWYKVLNHEYFDEIGLRFNILDKAIYTENKLKSLE
                     EVESIKTYLGNIIESVKFDVNYIF"
     gene            complement(12473..13132)
                     /locus_tag="PC471_002836"
     CDS             complement(12473..13132)
                     /locus_tag="PC471_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HDF2789948.1"
                     /translation="MTVVLIRSIILYITVLIALRVMGKGEIAEMNCFDLVITLLIAEV
                     ASVPMENNNIPIINGVAAISGLVIMQTLISFLSLKSRKLSSFLSGKPSVLIDKGKIVY
                     KELKKERISIDELLEQLRIQGYFNLKDVQYAILETDGNLSVVPASSYNSTPPRAFNHL
                     PIPLILDGRIINKNLDIAQKDTNWLMGILKSNHIETFKDVLICVLDENDKIFIQNKKG
                     D"
BASE COUNT         5365 a         1321 c         2291 g         4376 t
ORIGIN      
        1 aaccatgaac atttgttttg tgctatcata tttatataag ggaattgtca taaaaaatat
       61 cttgttcata cagttttata tagaaaaaag aaatgaatct tttctttata taagtatatt
      121 ttgtaaattg cctctatatt tatagtttaa ataatagtgg tattaaaaat ctgacaacaa
      181 aaataaaact ttcaaaaaat taaatctaaa aagaatagga gataaatatg agtattgaag
      241 atttatatga ttttgaatgt gttcctttga acaatgagat tgatattgat gttttatttg
      301 aaaagatgat agaagatgga aaagaaaaag aatatacacc aattatagtc attgatgaaa
      361 aagtagggct attaagaaaa aatatagata tgattactaa agagtatggt tctcttgaaa
      421 actatagaga gtattgttta acaaaatata atgaaattga tgtaaatcaa ttttttgatt
      481 ttaggaagag tgagatttca gaattattaa aggagctaat tgatttagaa gatgattttt
      541 atgattataa acattttaga ccaattatga atatagatat atttcatgaa tataacaaag
      601 tttacatagc taaaattcca acaattaaac catatgaagt atttgcatac atacctattg
      661 gcggatttaa tgattgtcct agagatgaag aacatatagc tattgcaaaa tattggtatg
      721 aaaagcatgg aatctttcca atcgcaattg gatgtgatac agttcaatat tctgtaaaaa
      781 atttaactaa agatggtaaa aaatttgatg aattatgtat tgaattagta ttttattgtg
      841 aggatattat tatacaaggg tatgaaacct taaaggctct taaggatgta ttaaaacgct
      901 ctacaatagt gcttttttgg tgggattgat attccatcat ttagatagta ttaaattgtt
      961 agtcaccaag aatccacttg ctttagttat ggggagtgtg tcaaactagg tttttataca
     1021 cagtgtagca tagtaatatg tttaaattac aaaggttaaa aaaatacttt aatattttta
     1081 atattataat taatagattt aaaggtaatt ataatttata aaataaatac tttatatgtt
     1141 ataatcatta agaatgtaaa aaaataatat atagttttat agttcatatt tttccagata
     1201 tggaggtaaa ttttgtgaag gaaaaagaaa aattagataa attaaaaaaa atgttacttg
     1261 aattaggcag tgttgtagtt gcttattctg gtggagtaga tagtaatttt ttgcttaagg
     1321 tcgcaaaaga tacattaggt gaaaatgtta tagctgtgac tattcatgca atgatgcatt
     1381 caagtaggga aattgaagag gctaaacaat atactcaaaa ttttggtgtt aaacatatca
     1441 tattaaatat agaaaacttt gatttaaaag aatttaaaga aaatggtgtt gatagatgtt
     1501 atcattgtaa aaaatatata ttctctaaaa taaaagaggt agcaaaagaa cataatataa
     1561 agtacatagt agatggaaca aatatagatg atttaggaga ctatagacct gggcttaagg
     1621 cattgagtga attaggagta attagtccat taaaagatag tagtttaaaa aaagaagaaa
     1681 ttaggtcgct ttcaaaaaca ttaggattaa atacttttaa taaaccatca tttgcatgtc
     1741 ttgcgagtag gataccttat ggagttgaaa taacagatga aaatttaaga atagtagaaa
     1801 agagtgaaga atatctatcc aatttaggtt tttctcaatt tagagtgaga atgcatggag
     1861 atatagctag aatagaagtt ggacaggaag aactaggtaa attttttgaa aataacaatt
     1921 ttaataaagt tgatactaaa ttaaaaatat ttggctttaa atatgttaca ctagatatgt
     1981 caggatataa aatgggaagt atgaatttaa atgtgtagat tattttaaaa ataaataagt
     2041 tttatagtta atataaggaa acaggaggtt ttatgaaaaa ttatacgtta atatcgccat
     2101 gtttttttgg aatggaaaaa atgctggcta gagagattac taatttagga tatgaaataa
     2161 taaaaacaga agatggaaga ataacatata aaactgatga gtttggaata gcaaagtcaa
     2221 acatgtggtt gagatgtgct gaaagagtac atcttaaaat tgctgaattt gaggcaaaaa
     2281 gctttgatga attatttgaa aatacaaaaa gaataaactg gtctagatat ataccttatg
     2341 gagctcaatt tcctatatca aaagcttcct ctataaaatc aaaattatac agtacaccag
     2401 atgtacaagc catagtaaaa aaggctatag tagaaagctt aaagaaaagt tatttggagg
     2461 atggtttgct taaagaagat aaggagaaat accctatttt tgtatttata cacaaagata
     2521 aagtaaccat ttctatagat acaacaggag atgctttgca taagagagga tatagagaaa
     2581 aggcaaataa agctccaata agagagactt tagctgctgg gcttatttat ttaactccct
     2641 ggaaagcagg tagagtttta gtagacccta tgtgtggttc tggtactata ttgattgaag
     2701 ctgctatgat tggtataaat atggcacctg gcttaaatag agagtttata tcagaaaagt
     2761 ggagaactct tgataagaaa atatggtggg atgtaagaaa agatgctttt aataagatag
     2821 ataatgaatc taaatttaag atttatggat atgatataga tgaagaatct atagatatag
     2881 ctagagaaaa tgctgaaata gcaggtgtag atgaatatat agagtttaat gtaggagatg
     2941 caactcaatt taaaagtgaa gatgagtttg gatttataat cactaatcct ccttatggag
     3001 aaagattaga agataaagac agtgttaaac agttgtataa ggagcttggt tatgcattta
     3061 gaaaattaaa aaattggtcg tattatttga taacttctta tgaagacttt gaatatgagt
     3121 ttgggcaaaa agcagataaa aaaagaaaat tgtataatgg tatgttaaaa actaactttt
     3181 tccaatatcc gggtcctaag cctcctagaa ataataaata agttttacat gatttatttg
     3241 taaaatataa aattttatta aaataagtaa aacattatga tgccttgaaa cgtctaatta
     3301 ttgtgaggag atttttgtga acaaatttaa agatttatag gattaggagg agttaaatga
     3361 aagtgttaaa gcttattact gtagtagccc tgatgatgtt atttgttact ggatgtaata
     3421 taaaaactga atctccagaa caattagcga agatacctga ctatgatgat acaaaaaaag
     3481 tattgtatga tggtattgac cagttactaa aaccagattc atctatgata ttgccttcta
     3541 atacaaaaga agtaggaaaa ataaataagg tagatttaaa cagcgatgga attgatgaat
     3601 tagtagtatt tgaacaaaaa gaagatttaa gtaataatgt gagtcaggta gggtttgtaa
     3661 ccttgagtta caatggagaa aaatacgtac ttggagacca ttttttagaa aatggcgaat
     3721 caatagaata tgctaatttt tatgatttgg actcagatgg atataaggaa gttatcttat
     3781 tggttaaatc caaagacaaa acgaatatgc atatatataa agtagtagat aatgaaataa
     3841 caaaaatata tgacttggat gcatcatggc ttcaaaataa agaagatttt aatgatatga
     3901 aagtaaaaat tggatatata gatggagatg acaagttaga catattgatg cttcatttga
     3961 ataataagac taatgaaatg tttgcaagtg ttgcaaattt tgatgggaaa atgaaaatca
     4021 aagattctgt taagtttgaa aatgtaaaaa acttaagtga attatatata acattaggta
     4081 atgttgcttc atctgttgga aattcttctg cagctataaa aggtattgta cttgatattc
     4141 ctatattgaa agataataac tatattacgc agatacttta tatgaaagat gggaaaatta
     4201 acaaagcttt tagtgattat gataaaacta ttactaaatc ttattatata cctgtagatg
     4261 atattgacaa agataagatt atagaaatac ctatagttgc aggcagtaca ggtaataata
     4321 aaaatactta tagttctaaa tcatctgcaa ctataagttg gtatagatgg aatggaaaag
     4381 aaggtgctga ctcaagttta atatttacaa gtcagatata ttataattat aaatataatt
     4441 ttaaattatt tattccaaat aatttatttg ataaaatact tgttgaacag gaatttgttg
     4501 gagaaaaagc agtatttaaa ttttactatt ttgactattc agaccgtaag aatttattta
     4561 atatagtagt agagagtaaa aataaacttg aagatagaaa aaatacaggt actcaaaatt
     4621 ctattgtact tcaagagagc gatgaatata ctttcttact agttgtaaac aatgcaaatg
     4681 aaatggataa gctagatatg actatagacg cattaaaaga atatttctca ttaatatatg
     4741 gttaatatta tactcaattg gaggttttat tttatgaagg aaaagatact tatactagaa
     4801 gatgaaattg gtattagaag ttttgttagt ataaacttaa aaagggaagg ctatgaaata
     4861 gttgaggcag gtactggaag agaagctata gaaaaaatga caacagaaaa ggatattaca
     4921 atagctcttt tagatgtaat gcttccagat ataagtggta tagaagtatg taaatttata
     4981 agggagaatt ttgaccaagt aggtataata atgcttactg ctaaagctca agaagatgat
     5041 aaaatagaag gatttatctc aggagcagat gattacatca taaaaccatt tagtataaaa
     5101 gagcttttag tcagagtttc tgctcttctt agaagagtag caaaggatga ttcaagtgtt
     5161 aagtcgagtg agatagtttc tccaccattt atacttgata tagacaaaag aaaattattt
     5221 aaaaatggaa aggaaattga gcttacacca actgaatttt caatagtgaa atatctaata
     5281 tcaaatgcta agcagtcgtt aagtagagac cagatattag acgaagtatg gggaactaac
     5341 tatttatatg attttaaaat agtagatgta aatataagaa ggataagaaa taaaatagaa
     5401 gatgacccat caaaaccaaa atatatacaa actatttggg gttatggata ctgttttaga
     5461 aaggaagaat aatagtgtta cattttgaag gtctgagaaa aaaagtaata aaaaactatt
     5521 ttataataat tattataatg gttactctgt ttgaaggatt gttcatgttt tacatacaaa
     5581 actactatta tgattctgtc aaacagctac ttgaatctga aataaaatat gctgatgaat
     5641 ataatgctat tacaatggaa actacaagtt ttgagaaaaa agtaaaaaat atatttgata
     5701 aacaaccctt aactaaaaat tcagaatttg gaatttcaat aatagataaa gataaaaata
     5761 ttatattaga ccaatatggt tttaaaagta aagaaaaagc aaactatgaa gatgttaaca
     5821 atgcattaaa agatataaaa actaaaaatt taacacctta tacttataga ataccagata
     5881 caggggagca tgttatgagt atctccttac cacttaaggt aaataatata atagaaggtg
     5941 ttgttagata tacagtatca ttagatgcta ttgataatgc aatattaaag caagctacat
     6001 ggcttatatt agcaggaata tttatactta ttatagctat actaataagt cttaaatttg
     6061 ctgagactct tataaaacca ttacgagagc taaagaaatt tgcaaatgaa ttggctgtag
     6121 gaaattataa tataaaatta gaaaagatga agattgtaga tgatgaaatt ggtgatttag
     6181 cacagacatt tgaacacatg gcacatgaga ttgacaaaag tgaaaaatta aaagaggaat
     6241 ttatatcatc tgtatctcat gaactaagga ctcctcttac atctataaag gggtggagtg
     6301 aaaccttagg ttatgaaagt ataacaagag aagaactaga cttaggttta ggcattatac
     6361 aagatgaaac agaaagactt ataaaactag ttgaagaatt attagacttt tcaaggcttt
     6421 cttcagacag gataaaactt catgttgata tagttgatgt tgaaggttta attgtaggtg
     6481 ttgtaaatca acttaaagtt aaagcagctg aaaaagatat atcactttta tttgaatttg
     6541 aaaatgaatt tatagagaac attcaaggag ataaaaatag attaagacag gttctcataa
     6601 atttaataca aaattcattt aaatttacga gtcaaggtgg ttacataaaa gttgtagctt
     6661 ctcaagatga agagataaca accatatcag tagaggataa tggttcaggt atagaaaagc
     6721 aaaatttaaa taaagtgtta gataaatttt tccaagaaga ttataataag gctggtagtg
     6781 gattaggact agcaattagt aatgaaattg taaaacttca tggtggaaga atgaaaatag
     6841 aaagtgaaaa gaatgttgga acaaaaataa cttttaatat aaaaaataaa tttgctaaac
     6901 aagcataaat tcttagaaag aggattaaga tgagaaacat aaaaatgata gtagcatacg
     6961 atggttcaag atataaagga tatcaaaaat taggtgacaa taatatgact attcaagaaa
     7021 aacttgaaaa tgttttaagt aaaatgacta atgaaacagt ggagataata ggttctggaa
     7081 gaactgatat gggagctcat gcaagaggac aagtggtaaa ttttagaact aattgtatgg
     7141 attctttgga taaaattcaa aaatatttat atgaatattt accagaagat atagttgtaa
     7201 aaactgttga agaagttgat gaaagatttc attcaagata caatgtaaaa tcaaaaactt
     7261 atatgtataa aattgacaat aataagtatc atagtccttt tataagaaaa tatgctactc
     7321 atgtaagtaa aaaattagat ttagatagaa tgagaaaagc aagtgagtat ttagtaggag
     7381 aacatgattt taccagtttt gcttcttcta aatctaagaa aaaatcaaat gtaagagaaa
     7441 tttattctat aaatataaaa gaagatgata atgttataga aatatatgta gaaggaaatg
     7501 gatttttgta taacatggta aggataatag taggtgcttt aatagatgtt ggtctaaaaa
     7561 gaagagctcc acaagatatt aagtatatgc ttgaatcaaa agatagatgt caatcatcag
     7621 atactgcacc agctaaagga ctatgtcttt ggaaagtaac atattaatca attatataaa
     7681 actctcttta aaataaaaat aaaataaatt atatagttga cataaatgga aaaataaact
     7741 attatatatt ttataagaga gatataaaac tttatattta aatcctgtga gaaagagagt
     7801 aacttctatg tgttttatag agagctgagg atggtggaag cttggcaaat aagtattagt
     7861 gaaaagaact ttggaggata ctaattgaat ttagtatgtt agtaggtaat ctcgccttaa
     7921 gagaaagagt ggataagttt atcttatcaa ttagagtggt aacgcggata tatttcgtct
     7981 cttggttttt ccaagaggcg ttttttaata tatttaatta ttagtgttta tctggtatgt
     8041 tgaacattta ctaattgatt agctaagttt atcaataaga gtggtaccgc ggatatttcg
     8101 cctcttagta ttttaaaata ctaaggggtt tttttagtat taaaatgatt ttatgtgagt
     8161 ctaaattctc agagtcattc aatattaaga atttctataa aataaaaatt ataatttaag
     8221 attgggagga attaaaaatg aaggaaaaag tagtattagc atattcagga ggacttgaca
     8281 catcaatcat aataccttgg ttaaaggaaa attatgaaga tatagatgtt atagctgttt
     8341 gtggaaatgt aggtcaggaa gataaaatgg aagatgtata tgaaaaagct ttacaaagtg
     8401 gagcatcaaa ggcatatgtg gatgatataa gtgaagaatt tgtaactgaa actatattta
     8461 aagccgtaaa agcagaggct aaatatgaag gaaaatactt attaggaact tctttagcaa
     8521 gacctataat agctaaaaaa ttagttgaag ttgctcataa agaaggtgca aaatatatat
     8581 gccatggatg tacaggaaaa ggtaatgacc aagttagatt tgaagctaca attgcagctc
     8641 ttgacccgac tataaaagtt attgcacctt ggagaatttg ggatataaaa tcaagagaag
     8701 atgctataga ctatgctgaa aaacataata taaaggtaac agcaactaaa gctaaaatat
     8761 attcagtaga tgctaacctt tggcatgttt caacagaagg tggagatata gagcacctag
     8821 aaaatgaaca taaaaaagat gtttataaac aatgtgtaga cccagaagat gcatgtgatg
     8881 tagctgaata tgtagaagtt tactttgaaa aaggtgtacc taaaaaggta aatggtgaag
     8941 aattatctcc agttgcactg attcataagt taaatgagtt gggttgtaaa catggaattg
     9001 gtgttataga tatagttgag aatagacttg ttggtatgaa atcaagaggt atatatgaga
     9061 ctccaggagg aacaatcctt tatgaagcac ataatatttt agaaagtgct actttagaca
     9121 aagatacatt acatttcaaa caaatggtat cttataaata cggagaactt atttacaatg
     9181 gactatggta ttgtaaatta agagaatcta tagatgcatt tatggaacaa actcaagata
     9241 atgttacagg aacagtaaaa gttaaacttt acaaaggaaa tataaaacct gctggaatat
     9301 ttactgaaaa tgctctttat gatgaaggaa tatcttcttt tggaaatagt gaactttatg
     9361 accacaaaga tgcagaagga tttattaatt tattcacttt accattaaaa atcagagcta
     9421 tgaaagctgg aaaataataa aataaattat ttaagagtgt ataaaaacta tttttaggtt
     9481 tttatatgct ctttttttat ttgaatttat taacttatac agtaaatagt ataaaaaaat
     9541 atattatagt tccaaatggc ttaataatgt gaaaatataa gacctaaaat ataaatttta
     9601 taaatacaat agttattgac tctctcattg taagagggtt tatactgaat ctaatgaaaa
     9661 tttatagtga ggagatagat tatatgagaa aaataattga aattaaacat cattcacatg
     9721 attatgagtg tatgtggaat ggtatagaag atttatatat aaataaaacc ggtgaatgcc
     9781 ttccaaacga ttttttcttt ttactgtcag gttttggttc attttgttat atgaaaacaa
     9841 ataaagctga tttgaaaaga atggtagctt tgggagatgg aaggacaaaa cagatgtata
     9901 aatttttagc accaattgtt ggatttgaat acaaacatca tgagtttaaa aagtatgagc
     9961 aagcattaaa gaaagcaaaa tctgaaattg atgatggata tccagttatt ttaggagctt
    10021 tagatatgta ttatctacca tattataaga agctatatca taaagaacat attccttttc
    10081 attatatatt aatggtagga tatgatgatg aaggaatata tctgtatgat tgtggtagaa
    10141 cagaattatt acatctttct tatgataaat tacgtgaaag catgaattgc aattcccctg
    10201 gattaagcaa agaaaatact atttgcacaa taagaatgaa tacttcaaag aataaatata
    10261 acattgctag agaagctatt gctataaagc gtgatatgtt tttaaatcca cctaaaggat
    10321 ttttgggata taaaggtttt gaaaagttta ttaaagagtt gccacagtgg aacactgaat
    10381 tggataaaga tgattatgat aagattttga ccaatatggt tatgtttttt ggaacagtac
    10441 caacaattcc aaatgcaatc aaaggaatta aagaaccaga tactgttcaa tttaaaggtg
    10501 gatttgataa aatgagtaga atgctcaaca gtattgggga ggaatacaaa aacgactctt
    10561 ttatacaagt agctgatatt tttgataaaa ctgctattat aattgagaaa atatccaata
    10621 ttataataga ttacttaact caaacatgtg atgatacagg acaacttccg ttattgttct
    10681 ctgaggtctt agagcatatg aaaacaggat atttaatctt ggatgtataa aaaatatatt
    10741 gttgacccta acattacgta atagtgtaat atgaaaatat agcttgaata tattagtagg
    10801 tattgatggc tgtaaaatta aaatttgagg tgtggatatg agaacagtga aacaagtttc
    10861 tgatttaact ggaataagtg tgagggcatt acattattat gatgaaatag gtcttttaaa
    10921 accaaataaa attacagatg caggatatag actttatgat gatgaatcta ttaaaactct
    10981 tcaacaaatt ttatttttta aggagattga tattccttta agagaggtta aggaaataat
    11041 gtcaagccaa tattttgata aggttgaagc tctaaaaaat caaaaaaaat tacttatttt
    11101 aaaacgaaag agactggatg aattgataga gcttataaat caaactctaa gaggagaagg
    11161 aaatattgat tttaaagagt ttgatatgag cgagtatttt gatgttttag aagaatttaa
    11221 aagagagcat aggaataagg taattaaaat ttatggtagt gtagaaaaat acaatgaata
    11281 tatagaaaga gttaagtcta atgaagagaa aattgctaaa atggctataa aacaatatgg
    11341 aactattgag aagtttgcta aagctataaa aacaaatttt agcagtgata tattgaattt
    11401 aggagaaaaa tttgatagat ataaaaatga ttgtttaaaa gaaaaacatc caaaattaaa
    11461 agaattatat agaaagcttg tagaagattt aaataggaat ccttcttcaa cagaccttca
    11521 agaaatagca aaagagataa cagatatatc taaaaaagat tatgaaattt tctctatgga
    11581 tactggtgat gacaactggt attatatggt tcaaaattat ctagtaaatc ctatgtggat
    11641 agaagaagta gataaaaagt atggaagtgg agcaggtaag tttattggac aagtactaaa
    11701 gacttatcta agagatagaa aaccaaagat aaatacattg tatgaaaaac ttgtagaaga
    11761 tttgagtaga gattgttctt caagggaagt tcaaagcatt gttgaagaaa tagacaatga
    11821 aatgaaaaga agtaatgaat tttataaaat agacaatggt gaaagatact ttgattatat
    11881 gtcagagctt tatttacaag attctaacta tataaaagta actgataaga attatggtga
    11941 tggtgcatct aagtttattg gagaagcttt taagatttat tttgataata ataattgttg
    12001 aaaatttgga agtaaaaaat atgcaagttt tataaaagca caaaaataag gatgtagatt
    12061 agctccatct atatccttat ttaaagttat taaaatatat agtttacatc aaacttaaca
    12121 ctttcaataa tatttcccaa atatgttttt atactttcaa cttcttctaa agattttaat
    12181 ttattttcag tataaattgc tttgtccaga atattaaacc ttaatcctat ttcatcaaag
    12241 tactcatgat ttaaaacttt gtaccaatgg tttttttctt tattccaact cgtagatatt
    12301 gaatcagctt ctttttgagc tttttcccaa tcttcatttt ctatagattt ttctaaaaca
    12361 gatatattgt ctttatatcc ttctgtaaaa tcatatagtt tattgttaac ataaaatcca
    12421 aataatacaa ataagatagt ccatagtata acaaatgttg tagatttcaa tattaatcac
    12481 cctttttatt ttgaataaat attttgtcat tttcatctaa aacacaaata agaacatctt
    12541 taaaagtttc tatgtgatta gattttaaaa tacccattaa ccagttagta tctttttgag
    12601 ctatgtctag atttttattt attattctac catccagtat aagtgggata ggtaaatgat
    12661 taaaagctct tggaggagta ctattgtagc ttgatgctgg tacaacactt agattaccat
    12721 cagtctccaa aatagcatat tgtacatctt ttaggttaaa gtatccttga atcctaagtt
    12781 gttctaataa ttcatctata ctaattcttt cttttttaag ctctttatat acaatttttc
    12841 ctttatctat taatacagaa ggtttacctg ataaaaatga acttaatttt ctacttttta
    12901 atgataagaa tgaaattaga gtctgcatga ttacaagtcc agatattgct gcaacaccat
    12961 ttatgattgg tatattatta ttttccattg ggacagatgc aacttctgca attaatagtg
    13021 ttataactaa atcaaaacag ttcatttcag ctatttcacc ttttcccatg actctaagtg
    13081 caattagcac tgttatgtat aatataatac ttcttattaa tactactgtc atatctatca
    13141 attactcctt taaaaaatat ttacacttag ttagtatatc ctatataaaa aatttaattc
    13201 aaagtataaa aaaccttaaa taattggatt caactattta aggttttttt gataatttta
    13261 atattaaatt ttttatggat tttctgttac ttttatttat tttaataaca gattcaacaa
    13321 aactcttttt atctcctaat tttaagataa aat
//
LOCUS       DAMJYR010000103        13275 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.106, whole genome shotgun sequence.
ACCESSION   DAMJYR010000103 DAMJYR010000000
VERSION     DAMJYR010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13275)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13275)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13275
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.106"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(118..2118)
                     /locus_tag="PC471_002837"
     CDS             complement(118..2118)
                     /locus_tag="PC471_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDF2789949.1"
                     /translation="MKQSIAIVTDKITKLSSFLEENIRLVLGNYIDINHYYLEVLKEN
                     DKIQGDFVLVMNDDRLNSMRKHLVQDSKIIVIRRTLKENEIYGLFSIPNGTEVLVVND
                     TKETTLETISLFYKIGVKNLKFTPYIEGKIYKNIDIAITPGVKGKVPSYINKVIDLGN
                     RYIDISTFIEIINGLRIDVKEIRKNLMAYSESLISLDNGVKDNYRQLFLKIEEQDVIL
                     NLSKDGIIFTSADGIINTFNKEALKILGINENPQGKNIEDIISEDLNILLEDKEIIDE
                     VVFTNKKYINVNKKSIFSMGNKAGIYYNLQEITYIKKLEQNLTNKLREQGQIARYTFK
                     DIKTKNKNMVKCVELAKKISKSDLSVLIIGESGTGKELLSQSIHNASNRSNQPFIAVN
                     CAAVPDSLLESQLFGYEKGSFTGALREGKKGLFELANNGTIFLDEIGDMPPLLQTKLL
                     RVLQEKQVMPIGSHNVINIDVRVIAATNKNLLKMIREGKFREDLYYRLNVLPIDVPSL
                     RNRKEDIITLMNFFLKNNIRLSPEVITILEKYNWPGNIRELQNVASYVSLMCDSMVFR
                     DDLPPYIRVECANNYEDGFGYTRKEFEEILRILYERRESSSGIGRGFIIEKLIQEGIE
                     ISESKVRKVLSYLSECGYISSKSGRGGSQITSEGIKLYNNIN"
     gene            complement(2149..3867)
                     /locus_tag="PC471_002838"
     CDS             complement(2149..3867)
                     /locus_tag="PC471_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HDF2789950.1"
                     /translation="MNFRLPKKRLPKKIIFICIILISIILFIKYDQNGLKEREIINKG
                     KYMSESLVADKEYDEAKKVVKTSFESIPEKKYNKVYNEVWDMLKTISYVPDGTKIVID
                     SLEKMRESDVKLSDECRFNIEKRLASVYIMDNNYSKAVDLTVKSIKLAEKLGNNYEKA
                     RLDVDLASIFLKIGGYETGEKLIKDSFKIDIDEGEKNGMVRLYAIINLAEIYVEEGEY
                     DKAIEISSRIKDYKKFVNTDNYNDFDIMASIMQSNAYVGKNNIQKAKTFLEKADYLIK
                     SDRTVYILDKDMYYYMAMGNLEYKSENYNSAIDNYKKSLEISTKRKLTQEKIKNLNKI
                     LNIYEKQGNIESLNEYQKMLINEYKENKSIRDSETSFYIIDKVSNESKLFEKTKKEIK
                     YYKILFTVILIAILGSTFLYNRLKYFKAQNLHDGLTNVYNRKSFDIMYEKYREKDDNF
                     ALVMIDIDNFKLINDNYGHKFGDTVIKGITNTICVMLEQDDKVFRYGGEEFSVLIRNK
                     SGDEVADIIDSIRVAIANKKWNDDVTVTISAGVAHVSDSKDVLEEADKNLYKAKQLGR
                     NKVVYK"
     gene            complement(4093..5331)
                     /gene="fabF"
                     /locus_tag="PC471_002839"
     CDS             complement(4093..5331)
                     /gene="fabF"
                     /locus_tag="PC471_002839"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HDF2789951.1"
                     /translation="MNKRVVITGLGCVTPLGTGKEEFWSNIKSGVSGIDKITNFDAST
                     YQTQIAGEVKNFHPEEYISKKELKRLDKFAQFAIVSAKLAVEDANLDLDKVDRERFGV
                     IIGSGIGGVEAIETQHKILLEKGNKRVSSLFVPMMIGNMAAGQVSIFLGAKGPNTNVC
                     TACASGTHSIGDAFKVIQRGDADLMVAGGSEAAVTGLAFAGFCNMKAMSTRNDDPKTA
                     SRPFDKDRDGFVMGEGAGIVILEDLEHALARGAKIYAEVVGYGLTADAYHMTTPAENG
                     EGAARSMNMALKDGNVPLEEVDYINAHGTSTYYNDLYETMAIKTVFGEKAYDLCVSST
                     KSMTGHLLGASGAIEAVVCAMSIEDSFVPPTINIQEVGEDLDLDYVPNQGKEKNIRYA
                     LSNSLGFGGHNATIVLKKYV"
     gene            complement(5360..5584)
                     /gene="acpP"
                     /locus_tag="PC471_002840"
     CDS             complement(5360..5584)
                     /gene="acpP"
                     /locus_tag="PC471_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HDF2789952.1"
                     /translation="MFNKVVEIIIEQLGVEDKEITMETSLMKDLEADSLDAVEIIMAL
                     EDEFGIEIPDTEAENFKSIGDIVKYIEANK"
     gene            complement(5638..6387)
                     /gene="fabG"
                     /locus_tag="PC471_002841"
     CDS             complement(5638..6387)
                     /gene="fabG"
                     /locus_tag="PC471_002841"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HDF2789953.1"
                     /translation="MINLTGQVAVVTGGSRGIGKEIAKKLASFGADVVINYTSKEDEA
                     LKTKNEIESMGVKCTSIKCDVSKFDEVNQMIDSVVSEFGKIDILVNNAGITKDGLLMR
                     MKEEDFDRVIDINLKGVFNCTKAVTKPMMKKKYGRIINMTSVVGIMGNAGQTNYCASK
                     AGVIGFTKASARELASRNININAVAPGFIETDMTKVLSDDVKESTLANIPKKSYGKPE
                     DVANAVAFLVSDMSSYITGQVINVDGGMVMQ"
     gene            complement(6387..7337)
                     /gene="fabD"
                     /locus_tag="PC471_002842"
     CDS             complement(6387..7337)
                     /gene="fabD"
                     /locus_tag="PC471_002842"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HDF2789954.1"
                     /translation="MGKVALVFPGQGAQYVGMAKDLYENNAVAKSVIDEASDALNMDL
                     KNLMFNGNEEELSKTENTQPAMVTHSVAVLKAVQAQIDLKYNACLGLSLGEYSALVAA
                     DAIDFKDAVCLVKKRGKFMQETVPAGVGAMAAVLGLDRNVLENVVKDIQGGIVEVANY
                     NSPGQIVISGENEKIEEAMVKCKEAGAKRAVKLNVSGPFHSSMLKEAGVKLASELEKV
                     NITKPKVDVVANVNADYYKNEGKDLLIKQVSSSVLWEDSIERLLNDGYDTFIEMGPGK
                     TLKAFIKKIASNKKANVNIYNIDGIDSLNEFVQNYRNGEI"
     gene            complement(7353..8282)
                     /gene="fabK"
                     /locus_tag="PC471_002843"
     CDS             complement(7353..8282)
                     /gene="fabK"
                     /locus_tag="PC471_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-[acyl-carrier-protein] reductase FabK"
                     /protein_id="HDF2789955.1"
                     /translation="MNKICKILNIKYPVIQGGMAWVATASLASAVSNAGGLGIIAAGN
                     APKEAIKKEIVECKKLTDKPFGVNVMLMSPFVDDIIDLIIEEKVQVITTGAGNPAKYM
                     DRLKEAGTKVIPVVPTIALAQRMEKLGATAVIAEGTEGGGHIGELTTMVLVPQVADAV
                     NIPVIAAGGIVDGRGIAASFALGASAVQVGTRFICSEECSVHSNYKNLVLKAKDRDAI
                     VTGRSTGHPVRTLKNKLSKEFLKMEQNGATPEELDKKGTGALRFATVDGDIEKGSFMA
                     GQSAAMVKEITPCKEIIEAMVNQAREIMPAIEL"
     gene            complement(8391..9377)
                     /locus_tag="PC471_002844"
     CDS             complement(8391..9377)
                     /locus_tag="PC471_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HDF2789956.1"
                     /translation="MNTKAGILGVGSYLPEQSYDNFHFEKIMDTSDEWISTRTGIKER
                     RFAKESEATSDLASKAALKAIECAKLNVEDIELIILATITPDMSLPSTACIVQDAIGA
                     VNATAFDISAACSGFVYGVTIAKQFVETGCYKNVLVIGAETCSKFLNYDDRTTAVLFG
                     DGAGAAVIGPVNEGGILSTHMGSDGKGKDCLKVPAGGSRLKASKETVEANLHTIEMAG
                     SDVFKFAVRKMAETSLRALEKANLNTTDIDYLVPHQANIRIIQASSKRLELDMKKVYV
                     NIDKYGNMSAASIPVALDEAYREGKIKKGDNVVLVGFGGGLTWGASVVKWTL"
     gene            complement(9403..10425)
                     /gene="plsX"
                     /locus_tag="PC471_002845"
     CDS             complement(9403..10425)
                     /gene="plsX"
                     /locus_tag="PC471_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HDF2789957.1"
                     /translation="MKIVIDGMGGDNAPKSNVEGAVNAIKEYQVDLIITGDKDLLEKE
                     FSNYEFDRNKLEIVHTTEIIENEDKPVKAIRSKKDSSMVVALNLVKEGKADAIISAGN
                     TGALLAGGLFVVGRIKGIDRPCLCSAIPNVKRGMTLIADCGANADCKPKNLVEFAAMS
                     NIYSRKVLGLENPKVALANVGLEEGKGNDLVKRSYEEIKKLDLNFIGNVEAREVINAY
                     TDIIICDGFTGNILLKSAEGVALSVMSLIKETFMASTKSKIGALLIKDDLRKLKSFID
                     YSEYGGAPLLGLNGGVIKAHGSSDAKAIKNAINQGIKFSKGKVVEDINQFISKYNEEN
                     KNNEDE"
     gene            complement(10436..10993)
                     /gene="fapR"
                     /locus_tag="PC471_002846"
     CDS             complement(10436..10993)
                     /gene="fapR"
                     /locus_tag="PC471_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription factor FapR"
                     /protein_id="HDF2789958.1"
                     /translation="MKKKSKAQRQKELIDMLKTDPFYTDEELSSLFDVSIQTIRLDRM
                     SLNIPELRERVKSIAETQSSKVKTLGVKEITGEIIDLSVGRLGISMLEVTQDMIYSKT
                     NTLKDTYIFSLADSLAMAIIDAPKVIMRVANVKSFKLIEQQDRLIAKAEVYRNIDKKH
                     YVKVVINNKAQEQIFRGKFIFEELD"
     gene            complement(11200..11376)
                     /gene="rpmF"
                     /locus_tag="PC471_002847"
     CDS             complement(11200..11376)
                     /gene="rpmF"
                     /locus_tag="PC471_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HDF2789959.1"
                     /translation="MAVPKRKTSKSNTKMRRAANSKMEATGFVSCPQCHEPKLPHRVC
                     PDCGYYKGKEVVSK"
     gene            complement(11401..11925)
                     /locus_tag="PC471_002848"
     CDS             complement(11401..11925)
                     /locus_tag="PC471_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HDF2789960.1"
                     /translation="MKVSIEKINRKETDKIDLNFCEKIDTISYCDEIYKLVSPVNLKG
                     KVSKTNKGLYLDIDVNFTIVDNCSRCLKEVEIPLEYSIQGFLVKEEDYDEDEFEEFDP
                     FIFDGEEIDLIDIIEQTLDFNVPHKVLCSENCKGLCQVCGANLNEEECSCSEITNDEE
                     YIDPRFAKLKDLFN"
     gene            complement(12018..13214)
                     /locus_tag="PC471_002849"
     CDS             complement(12018..13214)
                     /locus_tag="PC471_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HDF2789961.1"
                     /translation="MKILVLNCGSSSLKYQLIDMNNEEVLCIGLVERIGIEGSILKHE
                     KAGRDDKYVVEQPMKDHKDAIALVLEAVAHPEFGAVKEMKEIDAVGHRVVHAGEKFAT
                     SVVITPEVEEALKECIDLAPLHNPANIMGIDACKAILPDVPMVGVFDTAFHQTMPKSS
                     YLYGLPHELYTKYGVRRYGFHGTSHNYVSQRAAEILGKDIKDLKIVTCHLGNGASIAA
                     VDGGKCVDTSMGFTPLEGLIMGTRCGDIDPAILPFLMRKEGLDADGLDKLMNKESGVY
                     GMTGISSDFRDIEDAAKNGDERAQATLEAYVKKVQKYIGAYAAEMNGLDVVVFTAGVG
                     ENGKAIRADIASNMEFLGMKLDKEANDVRGKETVISTADSKVKMLLIPTNEELMIARD
                     TLRLVK"
BASE COUNT         3916 a         2615 c         1374 g         5370 t
ORIGIN      
        1 ttataaatta caatattctt tctgacataa tataaattaa tctctataaa tataaaagtt
       61 tattattatt taaatatcta aattcaaaat atgtgatttc aatatcaaat acttagctta
      121 atttatatta ttataaagtt ttatcccctc tgaggttatt tgacttcctc ctctaccaga
      181 tttagatgat atgtatccac attcacttaa ataagatagc acctttctaa ctttgctttc
      241 agaaatctca ataccttcct gtataagctt ttcgattata aaccctcttc caattccaga
      301 ggatgactct cttctttcat atagaatcct cagtatttcc tcaaattctt ttcttgtata
      361 accaaatcca tcttcataat tgttagcaca ttctaccctt atatatggtg gtaagtcatc
      421 tctaaaaacc atactatcac acattagact tacataagaa gcaacatttt gtaactctct
      481 tatatttcct ggccaattat atttttctaa aatagttata acttctggcg aaagtcttat
      541 attgtttttc aaaaagaaat tcataagtgt tattatatct tcttttctat tcctcaatga
      601 tggtacatct attggcaata catttagtct ataatacagg tcctctctaa attttccttc
      661 tctaatcatc tttagtaagt ttttgtttgt tgctgctatg accctaacat ctatgtttat
      721 aacattatgg gaacctatag gcataacctg cttttcttgt aataccctta gtaactttgt
      781 ctgtagcaat ggaggcatat ctccaatttc atccagaaat atagttccat tattagcaag
      841 ttcaaataga cccttttttc cttctcttaa agctcctgta aaagagcctt tttcatatcc
      901 aaacagttga ctttctaata gactatctgg aactgctgca caattaactg ctataaacgg
      961 ttgattactt ctattggaag cattatgaat tgattgtgat aataattctt taccagtacc
     1021 actttctcct attattaata cagaaaggtc tgatttagat atttttttag ccaattctac
     1081 acactttacc atatttttat ttttagtttt tatatcttta aatgtatatc ttgctatctg
     1141 accttgttct cttaatttat tagtaagatt ttgttctaat ttttttatat acgttatttc
     1201 ttgtaagtta taatatatcc cagctttatt ccccatagaa aaaatacttt ttttatttac
     1261 atttatatat tttttatttg taaataccac ttcatcaatt atttctttgt cttcaagtaa
     1321 aatatttaaa tcttcagaaa tgatatcttc tatgtttttg ccttgtggat tttcatttat
     1381 acctaaaatt tttaaagctt ctttattaaa tgtgtttatt ataccatctg ctgaagtaaa
     1441 tattattcca tcttttgata gattcaatat aacatcttgt tcttctattt ttaaaaacag
     1501 ttgcctataa ttatctttaa ctccattatc taaacttatt aaactctcag aatatgccat
     1561 taaattttta cgtatttctt taacatctat tctaagacca tttattattt ctataaaggt
     1621 agatatatca atatatctat ttccaaggtc gattacttta tttatataac ttggtacttt
     1681 tccctttact ccaggtgtta tagctatgtc tatattttta taaatttttc cttcaatata
     1741 tggagtaaac tttaaatttt ttactcctat tttgtaaaat aagcttatcg tttctaaggt
     1801 agtttctttt gtatcattta caaccaatac ttcagttcca tttggtattg aaaataatcc
     1861 atatatttca ttttctttaa gtgttctcct tatcactata atcttactat cttgtactaa
     1921 atgctttctc atactgttta atctatcatc attcattact agtacaaaat caccttgtat
     1981 tttatcattc tcttttaaaa cttccagata atagtggttt atatcaatat aattacctaa
     2041 aaccaaacgt atattttctt ctaagaaaga tgatagttta gttattttat cagttactat
     2101 agctattgat tgtttcatat ttatacctct tatttatata gtatataatt acttatatac
     2161 tactttattt ctaccaagtt gttttgcttt atataaattt ttatcagctt cttctagtac
     2221 atctttgcta tctgaaacat gagctacacc tgcacttata gtcacagtca catcatcatt
     2281 ccacttttta tttgcaattg caacccttat actgtcaatt atatctgcta cttcatcacc
     2341 agatttattt cttattaata cagaaaattc ttctcctccg tacctaaaga ctttatcatc
     2401 ttgttctaac ataacacaaa tggtatttgt tattcccttt ataacagtgt caccaaattt
     2461 atgtccataa ttatcattta tgagcttgaa attatcaata tctatcatca caagtgcaaa
     2521 attatcatct ttctctctgt acttttcata cataatatca aaactttttc tattataaac
     2581 atttgtaaga ccatcatgta aattttgtgc tttaaaatac ttcaatcggt tgtataaaaa
     2641 agtagaccct aatatagcaa ttaaaattac tgtaaataat attttataat attttatttc
     2701 ttttttagtt ttttcaaata gcttactttc gtttgatact ttatcaatta tatagaaact
     2761 agtctctgaa tctcttatac ttttgttctc tttatattca tttattaaca tcttttgata
     2821 ctcatttaaa gattctatat ttccttgttt ttcatatata tttaatatct tatttaaatt
     2881 ttttattttc tcctgggtta actttctttt agtagatatc tctaaacttt ttttataatt
     2941 atctattgca gaattataat tttcgctttt atattctaag tttcccattg ccatataata
     3001 gtacatatct ttatctaata tataaactgt tctatctgat tttataagat aatcagcttt
     3061 ttctaaaaaa gtctttgcct tttgtatatt atttttgccc acatacgcat ttgactgcat
     3121 tatagaggcc attatatcaa aatcattgta attatccgta tttacaaact ttttataatc
     3181 ttttatcctg ctacttattt caatagcttt atcatattcg ccctcttcta catatatttc
     3241 tgctagattt ataatagcat ataatctaac cattccattt ttttcacctt catcaatatc
     3301 tattttaaaa gaatccttta ttaatttctc tcctgtttca tagccaccta tctttaaaaa
     3361 tatgctcgcc aaatcaacat ctagtcttgc tttttcataa ttattcccca atttctcagc
     3421 caactttata gactttacag ttaaatctac tgccttagaa taattattat ccattatata
     3481 cactgatgct aatctttttt ctatattaaa tctacattca tcagataatt tcacatctga
     3541 ttctctcatc ttttctaaag aatcaatgac tatcttcgtt ccatctggaa catatgaaat
     3601 agtctttaac atatcccaaa cttcattata tactttattg tatttttttt ctggaataga
     3661 ttcaaaagaa gtcttaacca ctttttttgc ttcatcatac tccttatctg ccacaaggct
     3721 ttcagacata tattttccct tatttataat ttctctttct ttcaatccat tttggtcata
     3781 ctttataaac aaaataatac taataagtat tatacatata aaaattatct ttttgggtaa
     3841 ccttttcttt ggtagtctaa aattcaaaat tttagtcctc cttttgtata ttgtagaatg
     3901 aaaacttgca tatataaata acctcttaat aattaataat acaatatatc aaaatgattt
     3961 acaacatatc ctagacttaa atcttaataa aaaaatctcc taaaataatt taggagactt
     4021 taaatttaag ctaatataaa tttatacagt taaaacaagt gcttgttttt tagttttata
     4081 tttaatattt atttatacat atttttttag tactattgta gcattatgcc caccaaatcc
     4141 taaagaattt gatagtgcat atctaatatt tttttcttta ccttgatttg gaacataatc
     4201 taaatccaaa tcttcaccaa cttcttgtat gtttatagtt ggaggcacaa aactatcttc
     4261 tatactcata gcacaaacaa cagcctctat agcacctgat gctcctaata agtgaccagt
     4321 catagatttt gttgatgaca cacataagtc atatgccttt tctccaaata cagtttttat
     4381 agccatagtt tcatataaat cattgtaata agttgaagtt ccatgagcat ttatataatc
     4441 tacttcttct aatggaacat ttccatcttt caaagccata ttcatagacc tagctgctcc
     4501 ctctccattt tcagctggag ttgtcatatg gtatgcatca gcagttaaac catatccaac
     4561 tacttctgca tatattttag cacctcttgc taaagcatgt tctaagtctt ctaagataac
     4621 aatacctgct ccttctccca ttacaaaacc atctctgtct ttatcaaatg gtcttgatgc
     4681 agttttaggg tcatcatttc tagttgacat agccttcatg ttacagaatc cagcaaaagc
     4741 aagacctgtt acagcagctt cagaaccccc tgctaccata aggtctgcat cacctctttg
     4801 aattacctta aaagcatcac ctattgaatg agttcctgat gcacaagctg tacatacatt
     4861 agtgttaggt ccttttgctc ctaaaaatat tgaaacttga ccagctgcca tatttcctat
     4921 catcataggt acaaaaagtg aacttactct tttatttcct ttttccaaaa gaattttatg
     4981 ttgagtttct atagcttcaa ctccacctat tccagaacct attataacac caaaacgttc
     5041 tctatcaact ttatctaaat caagatttgc atcttcaact gctaatttag cagatactat
     5101 tgcaaattga gcaaatttat ctaatctctt caattccttt ttacttatat attcttctgg
     5161 atgaaaattc ttaacttctc ctgctatctg agtttgatat gtgctagcat caaaattagt
     5221 tattttatct ataccagata ctccagattt tatattactc cagaactctt cttttcctgt
     5281 tcccaacggc gtaacacatc caagtcctgt tattactact cttttattca tcggatacct
     5341 ccactttttt aaagctcgac tatttattag cttctatata cttaactata tctcctatag
     5401 atttgaagtt ttctgcttct gtatctggta tttctatacc aaattcatct tcaagtgcca
     5461 ttattatttc tactgcatct aatgaatctg cttctaaatc cttcattaat gatgtttcca
     5521 tagttatttc tttatcttct actcctaatt gctctattat tatttctaca actttattaa
     5581 acattgtgta cctcctaaaa ttttttattt taatagattt ctctactttt taaattatta
     5641 ttgcattacc attccaccat ctacatttat tacttgtcct gttatataac ttgacatgtc
     5701 actaactaaa aatgctacgg cattggctac atcttctggt ttaccataag atttctttgg
     5761 tatgtttgct agtgttgatt cttttacatc atcacttagt actttagtca tatctgtttc
     5821 tataaatcca ggtgctactg catttatatt tatgtttctt gatgctaact ctcttgcaga
     5881 agcttttgta aatccaatta cacctgcttt tgatgcacaa taattagttt gccctgcatt
     5941 acccataatt ccaactactg aagtcatatt tattattctt ccatacttct ttttcatcat
     6001 aggtttagta actgcttttg tacaattaaa gacacctttt aagtttatat ctataactct
     6061 atcaaaatct tcttctttca ttctcataag cagaccatct ttagttatgc ctgcattatt
     6121 aaccaatata tcaatttttc caaattcgct tacaacagaa tctatcattt gattcacttc
     6181 atcaaattta gacacatcac attttataga ggtacacttt acccccatac tttctatttc
     6241 atttttagtt tttagtgctt catcttcttt agaagtataa ttgattacta catcagctcc
     6301 aaaagatgct agtttttttg ctatttcttt tcctataccc ctagaaccac cagtaacaac
     6361 tgctacttgt cctgtaagat ttatcattat atctctccat ttctataatt ttgtacaaat
     6421 tcattaagag aatctatacc atctatatta taaatattga catttgcttt tttattggaa
     6481 gctattttct taataaatgc ttttaaagtt tttccaggtc ccatttctat aaaagtatca
     6541 tatccatcat ttaaaagtct ttctatagaa tcttcccaaa gaacagatga acttacttgt
     6601 tttataagca ggtctttacc ttcgttttta tagtaatctg catttacatt tgccaccaca
     6661 tcaactttag gtttagttat gttaactttt tctaattcac tggccaattt tacaccagct
     6721 tcttttaaca ttgatgagtg aaaaggtcca cttacattta atttaactgc cctttttgct
     6781 cctgcttctt tgcattttac cattgcttct tctatctttt cattttcacc agatataact
     6841 atttgtccag gagaattgta atttgctact tctacaatac ctccttgtat atctttaacc
     6901 acattttcaa gtacatttct atctaaacct aaaactgctg ccatagctcc aacacctgct
     6961 ggaactgttt cctgcataaa cttacctctt tttttaacta aacatacagc atctttaaag
     7021 tcaattgcat ctgctgcaac caacgcacta tactctccta aggataatcc taagcatgca
     7081 ttatatttta aatctatttg agcttgtacc gcttttaata ctgctacact atgtgtaacc
     7141 atagcaggtt gagtgttttc agttttagaa agttcttctt catttccatt aaacataagg
     7201 ttttttaaat ccatatttaa ggcatcacta gcctcatcta ttacactttt agcaacagca
     7261 ttattttcat ataaatcctt ggccatacct acatattgag ctccttgacc agggaatact
     7321 agtgcaactt ttcccatctt gctcctcctg atttacagtt ctattgctgg cataatctct
     7381 cttgcttgat ttaccatagc ctctataatt tccttacaag gtgttatttc ttttaccata
     7441 gcagcacttt gacctgccat aaatgaacct ttttctatgt ctccatctac tgttgcaaat
     7501 cttaaagctc ctgtaccttt tttatccagt tcttcaggag tagctccatt ttgttccatc
     7561 tttaaaaatt cttttgatag tttatttttt aatgttctta ctggatgacc agtacttctt
     7621 cctgttacaa ttgcatctct atcttttgct tttagtacta agtttttata gtttgaatgg
     7681 acagaacact cttcactgca aataaatcta gttcctactt gaactgcact ggcacctaat
     7741 gcaaatgatg ctgcaattcc tctaccatct acaattcctc cagcagctat tacaggtatg
     7801 tttacagcat cagcaacttg tggaactaag accatagtag taagttctcc tatatgtcct
     7861 ccaccttcag taccttctgc tattactgct gtagctccta gcttttccat tctttgtgcc
     7921 aaagctattg taggtactac aggaataacc tttgttccag cttcctttaa tctatccata
     7981 tactttgcag gatttccagc accagtagta ataacttgaa ctttttcttc tataatcaaa
     8041 tcaattatat catcaacaaa tggcgacata agcattacat ttactccaaa aggcttatct
     8101 gttaattttt tacactcaac aatttctttc tttatagctt cttttggtgc atttcctgct
     8161 gctattatgc caagtcctcc tgcattagat acagcacttg ctaatgatgc agtagctacc
     8221 catgccattc ccccttggat aactgggtat tttatattta atattttgca aattttattc
     8281 atacaattct ccttcacttg aaatcataaa agtaaacttt ttattattaa tcaactttat
     8341 tttgtgtata agtttaattt tgttaattaa atcattttag atattttata ctataaagtc
     8401 cacttgacaa ctgaagcccc ccaagttaga cctccaccaa agccaactaa aactacatta
     8461 tcaccctttt taattttacc ttctctataa gcttcatcta atgctactgg tatagatgca
     8521 gcagacatat ttccatattt gtctatattt acataaactt ttttcatatc aagctctaat
     8581 ctcttagaag aagcttgtat gattcttata ttagcttgat gtggcactaa gtagtctata
     8641 tcagttgtat ttagatttgc tttttcaaga gctcttaaag aagtttctgc catctttcta
     8701 actgcaaatt taaacacatc actacctgcc atttctattg tatgtagatt agcttctaca
     8761 gtctctttac ttgccttaag tcttgaacct ccagcaggta cttttaaaca atcttttcct
     8821 ttaccatcag agcccatatg tgtagacagt attccaccct catttacagg gccaataaca
     8881 gctgctccag ctccatctcc aaacaatact gctgttgttc tatcatcata atttaaaaac
     8941 ttagaacaag tttctgctcc tattacaagc acatttttat agcaacctgt ttccacaaat
     9001 tgctttgcaa tagttactcc atatacaaat ccagaacaag ctgctgatat atcaaatgca
     9061 gtagcattta ctgctcctat tgcatcttgt actatacatg cagttgaagg caaagacata
     9121 tcaggagtaa ttgtagctaa tataatcagc tctatatcct ctacgtttaa ctttgcacat
     9181 tctatagctt ttaaggcagc tttagatgct aagtcagaag ttgcttcaga ctctttggca
     9241 aatcttcttt ccttaatacc agttctagta cttatccact catctgatgt atccattatt
     9301 ttttcgaagt gaaaattatc atatgattgc tcaggtagat aacttcctac tcctaatatt
     9361 ccagcctttg tattcattat atattaaacc tccaaaacga gtttattcat cttcgttatt
     9421 tttattttct tcattgtatt tgcttataaa ttggttaata tcttcaacga cttttccctt
     9481 tgaaaatttt attccttgat ttatagcatt ttttatagct ttagcatcag aactaccatg
     9541 agcttttata actcctccat ttagacctaa aagtggagct cccccatact cagaataatc
     9601 tataaaactt tttaattttc ttaaatcatc ctttataagt aatgctccta ttttactttt
     9661 tgtactggcc ataaaagttt cctttataag actcataact gaaagtgcta caccttcagc
     9721 agatttaagt aatatattac ctgtaaatcc atcacatatg attatatctg tgtatgcatt
     9781 tataacttct ctagcttcta cattccctat aaaatttaaa tctaattttt taatttcttc
     9841 atatgacctt ttgactaaat cattgccttt gccttcttct agtccaacat ttgctaatgc
     9901 taccttagga ttttcaagac ctaaaacttt tcttgaataa atattactca tagctgcaaa
     9961 ttcaactaga ttcttaggtt tacaatctgc atttgcgcca cagtctgcta taagtgtcat
    10021 tcctcgcttt acatttggta ttgctgaaca taagcaaggt ctatcgattc cttttattct
    10081 accaacaaca aataatccac cagcaagtaa ggctcctgta tttccagctg atattatagc
    10141 atctgccttt ccttctttta caagatttaa tgctactacc atagatgaat cttttttact
    10201 tcttatagcc tttactggtt tatcttcgtt ttctataatc tcagtagtat gaactatctc
    10261 taatttattt ctatcaaatt cataatttga aaattccttt tcaagtaaat ctttatctcc
    10321 tgtgattatt aaatcaacct gatattcttt tatagcattt actgcgcctt ctacattaga
    10381 tttaggtgca ttatctccac ccattccatc aattactatt ttcataattt ccctcctaat
    10441 ctaattcttc aaagataaac tttcctctaa atatttgctc ttgggcttta ttgttgataa
    10501 caacttttac ataatgcttt ttatctatat ttctatacac ttctgctttt gctataagcc
    10561 tatcttgttg ttctatcaac ttaaaacttt ttacatttgc aactctcata ataaccttag
    10621 gtgcatctat tatagccatt gccaatgaat ctgctagaga aaaaatatac gtatctttta
    10681 aagtattagt cttagagtaa atcatgtcct gtgtaacttc taacatagat atacctaatc
    10741 gccctacaga taaatcaatg atttctcctg taatttcctt tactcctaaa gtctttactt
    10801 ttgaactctg agtttctgct attgatttga ctctttctct tagctctggt atgtttagac
    10861 tcattctatc taatcttatg gtctgtatac ttacatcaaa cagacttgat aattcttcat
    10921 cagtataaaa aggatctgtc tttagcatat ctataagctc tttttgtctt tgggctttac
    10981 ttttcttttt catgttccct ccaaatttta tagctgtaat tagtaccagc tactaatagt
    11041 agtatatagt aacacacaaa aattttcaat acttatatta ttatttttct attttttaat
    11101 atgcataaaa tccacttatt ttctctagta aattatctta tttttttata tgtacaaaaa
    11161 aaacatgtag agtatactct acatgctttt tagaagctat tacttagaca caacttcttt
    11221 acctttataa tatccacagt ctggacacac tctatgtggt aattttggct catgacattg
    11281 tgggcaactt acaaatcctg ttgcttccat ctttgagtta gctgctcttc tcatttttgt
    11341 gtttgattta gacgttttac gctttggtac tgccatttac gccacctcct tagtcaaaat
    11401 ttaattaaat aaatctttta atttagcaaa acgaggatct atgtattcct catcgtttgt
    11461 aatttcactg caagaacact cttcttcatt taagtttgcc ccgcatactt gacaaagacc
    11521 tttacagttt tcactacaaa gaactttatg aggtacatta aaatctaatg tctgttctat
    11581 tatatcaata aggtcgattt cttcaccatc gaatataaaa ggatcaaatt cctcaaactc
    11641 atcttcatca taatcttctt cttttactaa aaaaccttgt atagaatact ctagtggtat
    11701 ttctacttct ttaagacatc ttgaacaatt gtcaacaatt gtaaaattga catctatatc
    11761 tagatacaaa cctttattag tctttgacac tttccctttt aaatttacag gtgatactaa
    11821 tttgtaaatc tcatcacaat aactaatagt atcaattttt tcacaaaaat tcaagtctat
    11881 tttgtcagtt tcttttctat ttattttttc tatgctaact ttcattaagt tcacctcaac
    11941 atatcaccaa atttattata caaatctagc atttgtttgt caagtatttt tacttgatag
    12001 gcataggttt aatttaatta ttttaccaat cttaatgtat ctctagctat cattaattct
    12061 tcattagttg gtattaaaag catttttact ttagaatcag cagtagatat tactgtttct
    12121 tttcctctaa catcattagc ttctttatct aatttcattc ctaaaaactc catgttagaa
    12181 gcaatatctg ctcttatagc ctttccattt tcaccaacgc ctgcagtaaa tactacaaca
    12241 tccaatccat tcatttcagc agcataagcg cctatatatt tttgtacttt tttaacatac
    12301 gcttctaatg tagcttgagc tctttcgtct ccatttttag ctgcatcttc tatatctctg
    12361 aagtcactag aaattccagt cattccatat actccagatt ctttattcat taatttatcc
    12421 aatccatcag catctaaacc ttcttttctc attaagaatg gtaatatagc tgggtctata
    12481 tctccacatc tagttcccat tattaatcct tctaatggag tgaatcccat agatgtatct
    12541 acacattttc caccatcaac agcagctata gaagcaccat ttcctaagtg acaagttact
    12601 atttttaaat cttttatatc ttttcctaat atctctgctg ctctttgaga tacataatta
    12661 tgagaagttc cgtggaatcc atatcttctt accccatatt tagtatatag ctcatgaggt
    12721 aaaccatata agtatgaaga tttaggcatt gtttgatgga aagcagtatc aaatacacct
    12781 accattggta catctggaag tatagcttta caagcatcta ttcccattat gtttgctgga
    12841 ttatgaagtg gagctaaatc tatacattct tttaaagctt cttctacttc tggagttatt
    12901 actactgaag ttgcaaattt ttctccagca tgtacaaccc tatgtcctac tgcatctatt
    12961 tctttcattt ctttaactgc accaaattct ggatgagcaa cagcttctaa aactaatgct
    13021 attgcatctt tgtggtcctt cattggttgt tcaacaacgt atttatcatc tctacctgct
    13081 ttttcatgtt ttaatataga accttctatt cctattcttt caaccaatcc tatacataaa
    13141 acttcttcat tattcatatc tattaattga tattttaatg aagaactccc acagtttaat
    13201 actaatattt tcattattat ttcctcctta gattttttta taaataatat gtaataaatt
    13261 ttgatttgta ataaa
//
LOCUS       DAMJYR010000104        13177 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.107, whole genome shotgun sequence.
ACCESSION   DAMJYR010000104 DAMJYR010000000
VERSION     DAMJYR010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13177)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13177)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13177
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.107"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            162..1580
                     /locus_tag="PC471_002850"
     CDS             162..1580
                     /locus_tag="PC471_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HDF2789962.1"
                     /translation="MTELMIISGLVLLICITSSKVLYKFGVPILLIFILLGMLFGSDG
                     MVGIYFDNYELTKQLCSIGLIFIMFFGGFGTNWNMAKPVAIPSILMSSLGVIITAGVT
                     GMFCHMVIGTSLLEGLLIGAIAASTDAASVFAVLRSQKLNLKGSLASLLEVESGSNDP
                     IAYMLTLILLTLMNNSGVELIAPMVIKQIAFGLAIGFILAKLSVYILRHSNFEIEGFY
                     TIFITAIAILSYAFSEYLGGNGYLSVYISGIIIGNSRIPLKKSLVHFFDGISWIMQIM
                     LFFMLGLLSFPSKLPNVFGIAMAISIFMIVIARPLATFIVLAKFKFSNKEKLFISWVG
                     LRGAASVVFAIYAVTQGVAIENDIFHIIFFIALLSVGIQGSLIPTVARKLDLVDDNTP
                     VLKTFNDYDGEISSKLIEVNVDEGNKWANKSIIDSNIPDDILIVMIKRKEQVLIPKGS
                     TIIKTGDILVLSGDRIEELIQL"
     gene            1656..2468
                     /locus_tag="PC471_002851"
     CDS             1656..2468
                     /locus_tag="PC471_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDF2789963.1"
                     /translation="MFSIGMFSKINKITTKTLRYYEDIGLLKPEYVDEFTKYRYYTTE
                     QLPKLHQIITLKQMGLSLAEIKKAVENPEEVENILMNKEKEMLDVIKSEEYKIIKLRN
                     YLMNLKGEVNMNDVIIKSLPKVKVASMRKVLKGYDELFNLFPNVMAEEMMKTGCTCAV
                     PDYCFNIYHDGEYRETDMDVEMCQAINDLKEDTDILKFKELDAVENAVCILHNGPYST
                     LGKTYADAFKWIEDNKYKVVGNPRESYIDGIWNKESEEDWLTEVQIPVKRML"
     gene            2891..3508
                     /locus_tag="PC471_002852"
     CDS             2891..3508
                     /locus_tag="PC471_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HDF2789964.1"
                     /translation="MEIFAIGHSNYPYDKLINMIKKYDINCVVDIRETPYSKYNIQYN
                     KEAFNESLRNSGFLYIYMGKEFGAKRTNKDVYTQEGYADFEKVAKEDIFLNGIERLKK
                     GCQMGYRIVLLGAMQEPIRCHRSILVGKVLNKEGFDVKYIMHEGNLAYQEDIEESLLD
                     KYFSDRKQLSIDNLLGSALTREEMIQEGYKLANKEIGYRTEKLGK"
     gene            complement(3665..4117)
                     /locus_tag="PC471_002853"
     CDS             complement(3665..4117)
                     /locus_tag="PC471_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staygreen family protein"
                     /protein_id="HDF2789965.1"
                     /translation="MVNLNANNLNVNMVYPISSTAPIRFRRYTVSYLENMENIYLTIA
                     TYFDTLGLGTMNIDKVYGQWAWFTDNIYDLNLFVFVGNYPYQIAKNRYDTFVDILPVA
                     ITSIVNGDRVFLISNPCLLNSKITVRFISSHPTLNRTVDYGNIRDFIF"
     gene            4565..5923
                     /locus_tag="PC471_002854"
     CDS             4565..5923
                     /locus_tag="PC471_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HDF2789966.1"
                     /translation="MFIRKNVIKLAIPIMIEQTFVMLLGVFNTMMAGHIGEEAVSAVG
                     MVDSINNIFISVFAALSVGATVVVAQQIGKKDSEKVNETIKQALVSGVILSFTITILM
                     WVFRTGVINLFYGSAEELVKSNAKLYIEFTLFTYPFIATQQIANGILRGCGDVKKPMY
                     VTMFMNIVNVTLGYILIYGIDLNPLGINLQTPSYGITGAAISIAIARIIGCIVIIRAL
                     FKGNEFISMGKIFPFKIDVETQKSIFNIGIPAGVEQLLFNAGKLIVQVFIVTMGTSAI
                     ASNAIGMSIASIINVIGNALALSATTLVGQYIGRGDIKGAKSTLLYLTKFATICLFIV
                     GAIFIPIAPWISSMYTQNASVIDISTQLIRSDCLAMVIWPISFVLSAGLKGAGDTRYT
                     MMTAVIGMWIFRIFTGYVLGVVLEFGVLGIWIGMYTDWLVRGTLYCLRLRGTKWLKHK
                     VA"
     gene            6196..6576
                     /locus_tag="PC471_002855"
     CDS             6196..6576
                     /locus_tag="PC471_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDF2789967.1"
                     /translation="MINKIGKITLYVNNQEDAKLFWTEKLNFVVTYEKEMGPNMKWVE
                     VAPSKNEFTTFVLYDKNLMMKQNKEINVSHPSIILSTRDINGTYEQMANNEVEVGELM
                     EMPYGRMFSFKDQDGNDYLVREDK"
     gene            6701..6901
                     /locus_tag="PC471_002856"
     CDS             6701..6901
                     /locus_tag="PC471_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2789968.1"
                     /translation="MFKKMAVLKDIATKIGRKKAYELLEMVEGNDAFVAEVKIKKNGI
                     ESKKEEIMLKDNQKIILEYIEG"
     gene            7097..7825
                     /locus_tag="PC471_002857"
     CDS             7097..7825
                     /locus_tag="PC471_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDF2789969.1"
                     /translation="MLEIKNLSFNVESNNEELGIINDVSLSFERGKLIVITGPNGGGK
                     STIAKLIMGIEKATSGQIILDGEDITNLSITERAKKGIGYAFQQPPRIKGMTVENLLT
                     LAHGKPLSTDVCCQYLTDVGLCSKDYLNREVDNSLSGGEMKRIEIATLFARDLKVSIF
                     DEPEAGIDLWSFGKLNESFKKIHEESNQTIIIISHQERILELADEIIVLQDGSVKSHG
                     TKEAILPEIMCQVNSSCELMKDMN"
     gene            7847..8764
                     /locus_tag="PC471_002858"
     CDS             7847..8764
                     /locus_tag="PC471_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufD family Fe-S cluster assembly protein"
                     /protein_id="HDF2789970.1"
                     /translation="MNKIDLNLLEKVAELRGTPLGAYNIRKNGQGVERHSTENVTIIP
                     KTDKPGIDIIVKPNTKGENVHIPVIVTEAGVQDMVYNDFEIGENADVVIIAGCGIHNC
                     GCDDSEHNGIHRFFLRKGARLKYVEKHYGEGDGEGKRILNPVTIVNMDEDSYCEMETI
                     QIKGVDSTKREMDANLGKGAKIVVFEKLLTHGNQVAESNMKVILEGENSSAQVVSRTV
                     GQDNSKQKFNPCVIGDSLCSAHVQCDSIIMGNSQISAIPEIAANHPDALLVHEAAIGK
                     IAGDQILKMMTLGLTEEEAEQQILNCFLK"
     gene            8906..10090
                     /locus_tag="PC471_002859"
     CDS             8906..10090
                     /locus_tag="PC471_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HDF2789971.1"
                     /translation="MKNLLIRNGTIISPTDGIKFEKRDILVLHGKIYAIGLNLKEKIR
                     KEKLLTNKEFEIINADNMYISPGFIDVHTHCYKRKLPTGMDSDSIGIEKGSTVIFDAG
                     TAGPSNYYDFKKKYMDECKTEVYSFLNVTNEGLNILNESTYLDVINFDKVESIVEKNI
                     EKIKGIKVKASKFQSNESGIDIIKKSKQVAKKLNLPLVVYIGEYPSYIDEVMNILGKG
                     DVVTPVYGNSTNGILKESGTLRKSVINAKNRGVLFDVCHGVDQFDFKVFKKAIKQGLE
                     PDLISTDMHIKNMKDVNYSLLNVINKIMELGISLEKCIEKVTEYPTKIFDLHGNKGRI
                     KIGGEGDFTIFTMEECNDIIKDYNNNELVLNKKLRLQYTVKSWMKSSEVYRHGYENTI
                     IN"
     gene            10197..10385
                     /locus_tag="PC471_002860"
     CDS             10197..10385
                     /locus_tag="PC471_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HDF2789972.1"
                     /translation="MKAFVDKDVCVACGACIGTCPDVFSMSDEGHSVAIEGEIPADLQ
                     DLCKEAEEGCPVSAITVK"
     gene            10534..11184
                     /locus_tag="PC471_002861"
     CDS             10534..11184
                     /locus_tag="PC471_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HDF2789973.1"
                     /translation="MKKNYEIVLFDLDGTLTDPKEGITKSIQYSLNSFGIKEDLENLD
                     QFIGPPLHDTFKEYYKFEDKKAKEAVEKYREYFADKGIFENKIYENMKEILEMLYKNG
                     KILLVATSKPTVFAETILRYFDIDRYFKYIAGSNLDGTRVNKNEVIQYVLDLCNVKDK
                     DKVIMVGDRKYDIIGAKKIGIDSIGVLYGYGSFEEISESEPTYIVENVESIKDILL"
     gene            11287..12225
                     /locus_tag="PC471_002862"
     CDS             11287..12225
                     /locus_tag="PC471_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDF2789974.1"
                     /translation="MKSILIMGGSDFIGSALAKRLIKCGYQIDILTNGKKEIDYNGFK
                     EHLICDRKVRKDMENIITGRKYDYIYDMTAYTKEDVSNLIDFISMDNLKKYIVLSAGA
                     VYKDSGRNIKEENEKGENENWGKYGLNKKEAEDFIINSPIPYIIIRPTYIYGENNNLY
                     REYYFFEKIEKNEKIPVPKGKQVSNQFIYIGDLVKVLESIMKNPHVREAYNVTNPQLI
                     SWDDLIYTCGEIIGKEPIIKYVDMEKVEFRERTYFPFRNIDFNLDINKLIEHGLYIPN
                     VLLKEGLTATYKWFSANKPKMHDRKMNKVESVMQIV"
     gene            12251..13102
                     /locus_tag="PC471_002863"
     CDS             12251..13102
                     /locus_tag="PC471_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HDF2789975.1"
                     /translation="MNSKIKYFLKFFILIFILIFIYSVAIEPSLLIVKKYNISENGAL
                     IKNDSIESKSESVKVVQISDTQIGSFYSTKNLKKVANKINTLNPDIIVFTGDLIDYSN
                     KNPSVDEITTILSSMNARLGKFSVFGNHDYMYKLPRYYRQIMKNSNFNLLVNENKKIK
                     LKDDKYINILGVDEILNGNPNIKYLESQIDNKSFNLLLAHEPDLVDMLSKDTMNLVLS
                     GHSHGGQIRLPIKGALVTPPYGRKYTKGFYDINGNHLYVSSGLGSTKLPFRFFNIPEI
                     IEFNIII"
BASE COUNT         5250 a         1275 c         2333 g         4319 t
ORIGIN      
        1 ataaatttaa gtataacact cacattttaa tgtattgtga aatttttgtt gaactttatg
       61 taccataact tataaaattt atgtcatgtt atatttgaat aagtaataaa attgtagatg
      121 taaattaatc tacaaaattt atatgaaatg gagttgatgc tatgacagaa ttaatgatta
      181 taagtgggtt agtactattg atttgtataa cttctagcaa ggtattgtat aaatttggag
      241 taccaatatt attgattttt atcttactag ggatgttatt tgggtctgat gggatggtag
      301 gaatctattt tgataactat gaactgacaa aacaattatg ttctattgga ctaatattta
      361 taatgttttt tggagggttt ggaacaaact ggaatatggc taaacctgtt gctatacctt
      421 ctatattaat gtcttcactg ggtgtaataa ttacggctgg ggttacaggt atgttttgcc
      481 acatggtaat aggaactagt ttacttgaag gtttattaat tggggcaatt gcagcttcaa
      541 cagatgctgc atctgtgttt gctgtgttgc gttctcaaaa actaaactta aagggttcac
      601 ttgcatcatt attagaagtt gaaagtggaa gtaatgaccc aatagcgtat atgctgacac
      661 taattctttt gacattgatg aataattctg gggtagaact tattgcacca atggtaataa
      721 aacaaatagc ttttgggtta gctataggat ttatactagc aaaattatct gtttatattt
      781 taagacattc aaacttcgag atagaaggat tttatactat atttataaca gctattgcaa
      841 tactttctta tgcatttagc gagtatcttg gaggaaatgg atatttaagt gtttacattt
      901 caggtattat tataggaaat agtaggatac ctcttaaaaa aagtttggtt catttctttg
      961 atggaatttc atggattatg caaataatgt tattctttat gttaggattg ttatctttcc
     1021 cttctaagtt accaaatgta tttggtatag caatggccat atctatattc atgatagtta
     1081 ttgcaagacc attagcaaca tttatagttc tagcaaaatt taaattctca aataaagaaa
     1141 aattattcat atcgtgggta ggtcttagag gtgcggcttc agtagttttt gcaatttatg
     1201 ctgttactca aggagtagcc atagagaatg acatattcca tataatattt tttatagcgt
     1261 tgctttcagt aggaatacaa ggttcattga taccaactgt agccagaaag ttggatttag
     1321 ttgatgataa tacacctgta ttaaaaacat ttaatgatta tgatggagaa attagctcta
     1381 aacttatcga agtaaatgtt gatgagggta ataaatgggc taataagagt ataatagatt
     1441 ctaatatacc agacgatata ttaatagtta tgataaaaag aaaggaacag gttttaatac
     1501 ctaaaggttc aaccattata aaaacagggg atattttggt attgagtgga gatagaattg
     1561 aagaattaat acaattataa tttattttgt attgacttta acctaagggg aaagtttaaa
     1621 cttaaattaa tcaaatctaa tgcgcaggtg atgagatgtt ttctatagga atgttttcta
     1681 aaataaataa aataactaca aagacattaa ggtattatga agatataggg ttgttaaaac
     1741 cagaatatgt agatgaattt acaaaatata ggtattatac aacagagcaa ttacctaaat
     1801 tacatcagat aataacttta aaacaaatgg gtttatcatt agctgagata aaaaaagctg
     1861 tagaaaatcc tgaagaagtt gaaaatattt taatgaataa agaaaaagaa atgttggatg
     1921 taattaaaag tgaagaatat aaaataatta aactacgtaa ttaccttatg aatttgaaag
     1981 gagaagtcaa tatgaatgat gtaattatta agtctttacc aaaagttaaa gttgcttcta
     2041 tgagaaaagt gttaaagggg tatgatgaac tttttaatct atttccaaat gtaatggctg
     2101 aagaaatgat gaaaacaggg tgtacatgtg cagttcctga ttattgtttt aatatatatc
     2161 atgatggtga atatagagag actgatatgg atgttgaaat gtgtcaagct ataaatgatt
     2221 taaaggaaga tacggatata ttaaaattta aagaattaga tgcagttgaa aatgcagttt
     2281 gtatattaca caatggtcca tattcaacat taggaaaaac ttacgcagat gcatttaaat
     2341 ggatagagga taataaatat aaagtggttg gaaatcctag agaatcttat atagatggta
     2401 tatggaataa agagagtgaa gaagactggt tgacagaagt tcagatacca gtgaaacgca
     2461 tgttataata tgtctacaaa gtataaataa gaataaaaaa ggtggtgtct caaagtaaac
     2521 attttagttt atgagacact ctttttcata tgaaatcaag tatattttta taattacgat
     2581 tatgaaaaaa ggcttatcta tatttttaga cagcctctta tttttttatt ataatagttt
     2641 tctaaagatg ctctatttat aaatttttat taaagacttt ataaataatt gtatttaata
     2701 tacaaaatat atataaatat tgatgtttta tatataaagt tgtatatttt tttaaggata
     2761 taaagtaaaa aaatatacaa tatcaagaaa aaaagatatt gggtatttaa agtaatactt
     2821 tatttaattt taagtaaatc gggtatctac atatatagat actaatttca aagaatagga
     2881 gggtgtttat atggaaatat tcgcaatagg acattcaaat tatccatatg ataagcttat
     2941 taatatgata aaaaaatatg acataaattg tgtagttgac attagagaaa ctccatattc
     3001 taaatataat attcaataca ataaagaggc atttaacgaa tctcttagaa attccggatt
     3061 tttatatata tacatgggaa aagaatttgg agctaaaaga actaataaag atgtatatac
     3121 gcaagaaggt tatgcagatt ttgagaaagt agcaaaagaa gatatttttt taaacggaat
     3181 agaaagatta aaaaagggtt gtcaaatggg ttataggata gttttattag gagctatgca
     3241 agaacctata agatgtcata gaagtatctt ggtgggaaaa gtattaaata aagaaggttt
     3301 tgatgtaaaa tatataatgc atgaaggaaa tttggcatat caggaagata tagaggaatc
     3361 attattggat aagtatttta gtgatagaaa gcaactgagt attgacaatt tattagggag
     3421 tgcattaacg agagaagaaa tgatacaaga aggttataag ctagctaata aagaaatagg
     3481 atatagaact gagaaactag gtaaataaaa atatacaaat agataaaata cagtacttat
     3541 cttaagtaaa ataatattat gaatttataa tagctaggca tgatgtgcca attaaaaata
     3601 ttctagttat ttaataatat cattaaattt agggtatttt ttaatagcac atcatgttgc
     3661 agttttaaaa aataaaatct ctaatattgc cataatcaac tgttctatta agagtaggat
     3721 gactagatat aaatctaact gtaatttttg aatttaacaa acaaggattt gaaattaaaa
     3781 atactctatc tccattgaca atagaagtaa tagctactgg caaaatatct acaaatgtat
     3841 catatctgtt tttagcaatt tgataaggat aatttcctac aaaaacaaat agatttaaat
     3901 catatatatt atcagtaaac caagcccatt gaccataaac tttgtcaata ttcattgtgc
     3961 ctaaaccaag tgtatcaaag tatgttgcta tagttaaata aatattttcc atgttttcta
     4021 aatatgaaac agtatatctt ctgaaacgta tgggtgctgt ggaagaaata ggataaacca
     4081 tatttacatt tagattattt gcatttaaat taaccaaagt atcacatcct aaaattagtc
     4141 tataaaatac aatatttaaa tggatgcgat tttgttacat ctaaagtgta atattttata
     4201 tattgaaaaa gtggtaaaag tttggatgat taaataatat tataaaaaat atataaaatg
     4261 tacttattgt tattgctaca cgatagtgat acaatattaa cgaaatatag ctacttttaa
     4321 taataacgga aaatatttat attaaattaa taaatatttt aaatgtggta tccataaaaa
     4381 ataataaaca taagataatt ataaagatat actgtgttca aatgatatta aacaagattt
     4441 atattatttg aaagcagtat attataatgc gaacaataca attctaaact ttgcattgtc
     4501 aatttacagt tcgataaact aattaaaaat taaatttata taaagaggga aaagaggaat
     4561 agggatgttt ataaggaaaa atgttataaa gttagcaatt cctataatga tagagcagac
     4621 gtttgtcatg cttttaggag tatttaatac aatgatggca ggtcatattg gggaagaagc
     4681 tgtatctgca gttggaatgg ttgattcaat aaacaatata tttatatctg tatttgctgc
     4741 actatctgtt ggagctactg ttgttgtagc tcaacaaata ggaaaaaaag atagtgaaaa
     4801 ggtgaatgaa actattaagc aggctctagt gtctggagta atattatcct ttacaataac
     4861 aatattgatg tgggttttta gaactggtgt aattaattta ttttatggtt ccgcagaaga
     4921 attggtaaaa tcaaatgcaa aattatacat agagtttaca ttatttacgt atccatttat
     4981 tgcgacacaa caaatagcaa atggtatttt aagagggtgt ggagatgtaa agaaacctat
     5041 gtatgtgact atgtttatga atatagtaaa tgtaacattg ggatatatac ttatatatgg
     5101 tattgattta aatcctctag gaataaattt gcaaacacca tcctatggca taacaggtgc
     5161 agctatatct attgctatag ctagaataat tggatgtata gtaattataa gagctttatt
     5221 taaaggaaac gaatttatat ctatgggtaa aatatttcca tttaagattg atgttgaaac
     5281 tcaaaagtct atatttaata taggaatacc agctggagtt gaacaactac tttttaatgc
     5341 aggtaaattg attgttcaag tatttatagt tactatggga acatcagcaa ttgcatccaa
     5401 tgcaatagga atgtcaattg catccataat aaatgtaatt gggaatgctt tagctttgtc
     5461 tgctacaacc ttggtgggac agtatatagg acgtggagat attaaaggag ctaagagtac
     5521 tttattatat ttaactaaat ttgcaacaat ttgtttattt atagtaggag ctatatttat
     5581 accaattgca ccctggattt caagtatgta tactcaaaat gctagtgtaa ttgacatctc
     5641 aacacaatta attcgaagtg attgtttagc tatggtaata tggcctatat cctttgtatt
     5701 atcagcaggg ctaaagggag ccggtgatac tagatataca atgatgactg cagtaatagg
     5761 tatgtggatt tttagaattt ttacaggtta cgtattgggg gttgtattag aatttggagt
     5821 gttaggaatt tggataggta tgtatactga ttggcttgtt agagggactt tatattgttt
     5881 aagattaaga ggaacaaagt ggctaaaaca caaagttgca tagaatatat gaaatcactt
     5941 ttagatggat aactctattt agaagtgatt ttttatgtag ttattatcat aattataagt
     6001 atgatatata aggtgattaa attacataaa taaaggctat aatagtatgg atattataaa
     6061 ttatatattg agcagaatta cataatttgg cgataaatat tagaaatata tatctataga
     6121 aaataatata tatgggtata ttattaaata taaaaaatat taggtattta tttaataata
     6181 aaggtgggta ttgttatgat aaataaaata ggaaaaataa ctctctatgt aaataatcaa
     6241 gaggatgcaa agttattttg gacagaaaaa ttgaactttg tagtaactta tgaaaaggaa
     6301 atgggtccaa atatgaaatg ggtggaagta gcaccaagta aaaatgaatt tacaacattt
     6361 gtgttgtatg ataaaaatct aatgatgaag caaaacaaag agataaatgt atctcatcca
     6421 tcaataattt taagtacaag agatattaat ggaacttatg aacaaatggc aaataacgag
     6481 gttgaggttg gggaattaat ggagatgcca tatggaagga tgttttcttt taaagaccaa
     6541 gatggaaatg attatttggt cagagaggat aaataatttt acaaagtatt aaatttactc
     6601 aatatctgat ataattagag acaataatta ttataaacta gtctaggatt agaattatat
     6661 aattaaaaat taagtgtaaa ttttgagtag aggtgataaa atgtttaaga aaatggcagt
     6721 actaaaagat atagcaacta aaataggtcg taaaaaagcg tatgaactat tagaaatggt
     6781 tgaaggtaat gatgcctttg tagctgaggt aaagataaaa aagaatggaa tagaatctaa
     6841 aaaagaagaa attatgttaa aagataatca aaaaataata ttagagtata tagaaggtta
     6901 aattttaaat acatataagt tgatatacta aatttctgag atgagtttac ataaaactca
     6961 tcttttttac ttttcaataa ctttgtttta ttatttaata tacattttat ctccttagag
     7021 ttgctgatat ataaaaatta ctgatataat aaaagaatgg atattaatgt aatattttat
     7081 agaaaagagg ggtattatgc ttgaaataaa aaatctttcc tttaatgttg agtctaacaa
     7141 tgaggaacta ggaataataa atgatgttag cctttcattt gagaggggaa aacttattgt
     7201 tatcacaggt ccaaatggtg gaggaaaatc tacaattgct aagcttatta tgggaattga
     7261 aaaagctacg agtggtcaga taattttaga tggagaagat ataacaaatc ttagtattac
     7321 agaaagagca aaaaaaggaa taggatacgc atttcaacaa ccaccaagaa taaaaggtat
     7381 gacagttgaa aatttactta cacttgctca tggaaaacca ttgtctactg atgtatgttg
     7441 ccaatacctt acagatgttg gattgtgttc aaaagactat ttaaatcgtg aagttgataa
     7501 ctctctttct ggaggggaaa tgaaaagaat tgagatagca actctttttg ctagagattt
     7561 aaaggtttct atatttgatg aacctgaagc agggatagac ctttggagct ttggaaaact
     7621 caatgaaagt tttaagaaaa tacatgaaga gtctaatcag actataatca taatttctca
     7681 ccaagaaaga atattagagt tagctgatga aataattgtt ctacaagatg gttcagtaaa
     7741 aagccatgga acaaaagagg ctatattacc tgaaatcatg tgtcaagtta attcaagttg
     7801 tgaattaatg aaagacatga attaattaga aaggaaggca aattaaatga acaaaataga
     7861 tttaaatctt ttagaaaaag ttgctgaact tcgtggaact ccattaggag catacaatat
     7921 acgtaaaaat gggcaaggag tagagagaca ttcaactgaa aatgtaacta taattcctaa
     7981 aactgataaa cctggaatag atattatagt aaagccaaat acaaaaggag aaaatgtaca
     8041 tataccagta atagtaacag aggctggtgt acaggatatg gtatataatg attttgaaat
     8101 tggagaaaat gcagatgttg ttataattgc tgggtgtggt atacataact gtggttgtga
     8161 tgatagtgaa cataatggta tacatagatt tttcttacgt aaaggagctc gactaaaata
     8221 tgtagaaaaa cattatggtg aaggtgatgg agaaggaaag agaattttaa atccagttac
     8281 tattgtcaat atggatgagg attcatattg tgaaatggaa acaattcaaa ttaagggtgt
     8341 agattcaact aaaagagaaa tggatgcaaa cctaggtaaa ggagctaaaa tagttgtatt
     8401 tgagaaatta ttaactcatg gtaatcaagt tgcagaatct aatatgaagg ttatcttaga
     8461 gggtgaaaac tcttctgctc aagtagtttc tcgtacagtt gggcaagata attctaagca
     8521 aaagtttaat ccttgtgtta ttggggattc cctttgttct gcacatgttc aatgtgattc
     8581 aataattatg ggaaattctc aaattagtgc tatacctgaa attgctgcaa atcatccaga
     8641 tgctctttta gtacatgaag cagctattgg aaagattgca ggagaccaaa tactaaagat
     8701 gatgacatta ggtcttactg aagaagaagc cgagcaacaa atacttaatt gcttcttaaa
     8761 gtaaattaaa gattttattt aatttaatat aattaatata gtataatata gtgaataagt
     8821 ctaaaatatt tttattttta ggcttatttt ctttatgtaa gaaaagctat tagagaaatt
     8881 atattgtaat taggatgtgg aataaatgaa aaatttactt ataagaaatg ggacaataat
     8941 atctccaaca gatggtatca aatttgaaaa aagagatatc ttggttttac atgggaaaat
     9001 atatgctatt ggattgaacc ttaaagagaa aataagaaaa gaaaaattac ttacaaataa
     9061 agagttcgag ataataaatg ccgataatat gtatatttcc ccaggattta tagatgttca
     9121 cacccattgt tataagagaa aattacctac aggtatggat tctgattcaa ttggaattga
     9181 aaaaggttct acagttatat ttgatgcagg tacagctggt ccctcaaact actatgattt
     9241 taaaaaaaag tatatggatg agtgtaaaac agaagtatat tcttttttaa atgttacaaa
     9301 tgagggacta aatatattga atgaatcaac ttatttagat gttataaatt ttgacaaggt
     9361 tgagtcaatt gtagaaaaaa atatagaaaa gataaagggt ataaaagtaa aagcgagtaa
     9421 atttcaatct aatgaaagtg gtatagatat aattaagaaa tcaaagcaag ttgctaaaaa
     9481 attaaattta cccttagtgg tatatatagg agagtatcca tcatatatag atgaagtaat
     9541 gaatattttg ggaaaaggag atgtggttac accggtatat ggaaatagta caaatggaat
     9601 ccttaaagaa tctggcacat taagaaaatc agtaataaat gcaaagaata gaggagtatt
     9661 gtttgatgta tgtcatggag tggaccaatt tgattttaaa gtttttaaaa aagcaataaa
     9721 gcaaggttta gagcctgacc taataagtac tgatatgcat ataaaaaaca tgaaagacgt
     9781 aaactattct cttttaaatg ttataaataa aattatggaa cttgggatat ccctagaaaa
     9841 gtgtatagaa aaagtaactg aatacccaac aaagattttc gatttacatg gaaataaagg
     9901 cagaataaag attggtggag aaggtgattt tacaatattt acaatggagg agtgtaatga
     9961 tattataaaa gactacaata ataatgaact tgttttaaat aaaaaattaa gattgcaata
    10021 tactgtaaaa tcatggatga aaagcagtga agtttataga catggatacg aaaatactat
    10081 aataaactaa acaatagtga taaaataata aatatatgtt taaattggta tatattggat
    10141 aaatatctaa ggaaacaaca attaaaaaat aataatttta ggggaggtat tttagtatga
    10201 aagcatttgt tgataaggat gtatgtgtag cttgtggagc atgtatagga acatgtccag
    10261 acgtttttag tatgagcgat gaagggcatt cagtagcaat tgaaggagaa ataccagcag
    10321 atttacaaga tttatgtaaa gaagccgaag aaggatgtcc agtttcagct ataacagtta
    10381 aatagagctg tttaaattta tttttaaaat aaatatataa gatttaattg gtacttaagc
    10441 tatagtatta gaaatttcta ttactatagc tttttaaaat agataaactt aatatataag
    10501 atattacttt ttacaaatat ggaggactga aatatgaaaa aaaattatga aatagtatta
    10561 ttcgatttag atggaacact tacagaccct aaagaaggaa taacaaagtc tattcagtat
    10621 tctttaaata gctttggcat aaaagaagat ttggaaaact tagaccaatt tataggacca
    10681 ccattacatg atactttcaa ggagtattat aaatttgaag ataaaaaagc taaagaagct
    10741 gttgaaaagt atagagaata ttttgctgat aaagggatat ttgaaaataa aatttatgag
    10801 aatatgaaag agatattaga aatgctctat aaaaatggta aaatattatt agtagcaact
    10861 tctaagccaa cagtttttgc agaaactata cttagatatt ttgatataga tagatatttt
    10921 aaatatatag caggtagcaa tcttgatggt acaagagtga ataaaaatga agttatacaa
    10981 tatgtactag acctatgtaa tgtaaaagat aaagataaag ttattatggt tggagataga
    11041 aagtatgaca taattggagc taaaaaaata ggtatagatt ctattggagt attgtatggg
    11101 tatggttctt ttgaagagat tagtgaaagt gaacctactt atattgtcga aaatgtagag
    11161 tctataaaag atattttgtt ataaatatct tttatagaca aaaatattac aaaatggtgt
    11221 cgcatattta aaaatatgat attatagtta aggaagtcat ttaaaaaaga ggaggatgca
    11281 cttaaaatga aatctatttt aattatgggt ggaagcgact tcataggttc tgcacttgct
    11341 aagcgcttga ttaaatgtgg gtatcaaatt gatatactta caaatggtaa aaaggaaata
    11401 gattataatg gttttaaaga acatctaatt tgtgatagaa aagttcgaaa agatatggaa
    11461 aacattatca caggaagaaa atatgattac atatatgata tgacagcata tacaaaagaa
    11521 gatgtaagta atctgattga ttttataagc atggataact taaaaaagta tatagtattg
    11581 tcagcaggag cagtatataa agatagtgga agaaatataa aagaagaaaa tgagaaaggt
    11641 gaaaatgaaa actggggaaa atatgggtta aataaaaaag aggctgaaga ttttattata
    11701 aatagcccta taccatatat aattattaga ccaacttaca tatatggtga aaataataat
    11761 ttatatagag agtattattt ctttgaaaaa atagaaaaaa atgaaaagat accagtacca
    11821 aaaggaaaac aagttagtaa tcaatttata tatattgggg atttggtaaa ggtattggaa
    11881 agcataatga aaaatcctca tgtaagagag gcctataatg tgacaaatcc acagttaatt
    11941 tcttgggatg accttatata tacatgtgga gaaattattg gcaaagaacc tataataaaa
    12001 tatgtagata tggaaaaagt agagtttaga gaaagaacgt atttcccatt tagaaatata
    12061 gactttaatc ttgatataaa taagcttatt gaacatgggc tttatatacc aaacgtgtta
    12121 ctaaaagaag gtcttacagc aacatataaa tggttttcag caaataagcc aaaaatgcat
    12181 gacagaaaaa tgaacaaagt tgagtctgta atgcaaatag tttaaaaaaa taaataagta
    12241 ggtagatatt ttgaactcaa aaattaagta ttttttaaaa ttttttatct taatatttat
    12301 tttgattttt atatactcag ttgcgataga gccgagtcta ttaattgtga aaaaatataa
    12361 tataagtgaa aatggagcat tgataaaaaa tgattcaatt gagagtaaat ctgaaagtgt
    12421 aaaagtagtt caaatttcag atactcaaat aggtagtttt tattcaacta agaatctcaa
    12481 aaaagtagct aataagatta acactttgaa tccagatata atagtattta ctggtgattt
    12541 aattgattac tccaataaaa atccaagtgt agatgaaatt acaactattt tatcaagtat
    12601 gaatgctagg ttgggaaaat tttcagtatt tggcaatcat gattatatgt ataaacttcc
    12661 taggtattat aggcagatta tgaaaaattc caacttcaat cttttagtaa atgaaaataa
    12721 aaaaataaaa ttaaaagatg ataaatatat taatatatta ggtgttgatg aaatattaaa
    12781 tggaaatcct aatataaaat atctggagag tcaaatagat aataaaagtt ttaatttatt
    12841 gttagcacat gaaccagatt tagtagatat gcttagtaaa gatacaatga atttagttct
    12901 atctgggcat agtcatggtg gtcaaataag attacctatt aagggagcat tagttactcc
    12961 tccatatgga aggaaatata ctaaagggtt ttatgacata aatggaaatc atttatatgt
    13021 gagttcgggt cttggaagta caaagttacc atttagattt tttaatatac cagagataat
    13081 agaatttaat attataatct aacataaaat atttagaaaa tataattttg ctttttataa
    13141 tatgtagatg tatatactgt aaaaatggat taaacca
//
LOCUS       DAMJYR010000105        13084 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.108, whole genome shotgun sequence.
ACCESSION   DAMJYR010000105 DAMJYR010000000
VERSION     DAMJYR010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13084)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13084)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13084
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.108"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            160..1041
                     /locus_tag="PC471_002864"
     CDS             160..1041
                     /locus_tag="PC471_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDF2789976.1"
                     /translation="MNLYHLRYFVTLAHLEHYTKAAENLSITQPSLSHAISLLENELG
                     VALFEKEGRNIVLTKYGKIFLKDVEKSLEILDSSVKSLKITGTGEGQIDLAFLRTLGT
                     DFIPDIVHKFLKSNPAKSIDFKFHTGVTTDIIQGLKERKYDIAFCSKLEKEKGIEFIP
                     VAKQDLVLIVPYSHPLAAKDTIDLKETIPYPQIVFNQRSGLRYIIDDMFKKINQQPNI
                     VYEVEEDQVIAGLVAKNFGIAVVPNMNMLSFTKVKVIQIIHPSWERNFYLAFIKDRYL
                     PPAIKNFKNFVIKNAQL"
     gene            complement(1249..2112)
                     /locus_tag="PC471_002865"
     CDS             complement(1249..2112)
                     /locus_tag="PC471_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3800 domain-containing protein"
                     /protein_id="HDF2789977.1"
                     /translation="MLSSYVKRVSKIIKKKYIMFIDESGRADINHEDPFTLTGVIFEY
                     KYAVSQGDYVCSLRKEMDAFKEYCFGTSNIGIHLTDISRKSRAFEKFDDSQIKLFYDE
                     LPLFLSRLECTIISVTVDKARLKKYYAPSKEPYVVAFIHVLQNFYSFVNNETVESARI
                     VIESRDDVSNLKVQKAFFDVFNNGTIYLDIDEELRDKVKGFIIAKKEDADYREGLEIA
                     DILCNPLSRARRGLIEANPKCMQYGEKNKIFKSVRNKIYTPTSVNDIRNWGFKEVPIV
                     EPVGPWLEKMR"
     gene            complement(2267..4201)
                     /locus_tag="PC471_002866"
     CDS             complement(2267..4201)
                     /locus_tag="PC471_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp22"
                     /protein_id="HDF2789978.1"
                     /translation="MKKSLKKYLVLALTLVLVFACGVPESSAASKHVIIVNSRKNTLG
                     YFVNNKLVKEFRVATGKKGSETPTGKTKVVNKIKNRPYYKGNIPGGSPRNPLGDRWMG
                     LALKGTYGDTYGIHGNNNESSIGKHISGGCIRMHNKDVRWLFDQVPVGSDVIIDYSND
                     SYVKIAAKYKINLNQTGWKTENGKKYYVKSDGTYQKNSWLKVNGKMYYFDASGVMQTG
                     WKTINNKKYYLGTDGARVSGWKVIDGKTYYFNSDGVMQTGWQEKNGKKYYLGSDGLAV
                     TGWQEIDGNKYYFDKTGIMQTGWQQIDGKSYYLDKDGKMLTGSQKIDGKDYTFNEDGT
                     INPTWDTIIGANRFDTAKKISSVGNWNADSSDTVILVNGNAIADGITATPLASSYDST
                     ILLTNTANLPTETVEEMKLLAPKTVILIGGENAISSKLEQEIKTTFNTETKRIAGQDR
                     YQTATRIAEELGNREEIKTAYMVSGNGEADALSVASKAGEEKQPIILVNKDGITEESY
                     KWLTERKLENAYFIGEPSAINDSVIAKMNDITTEDISGNRIYGDSRVDTNAKVIEKFY
                     GDTDLQAVLVSKSDALVDALSAGPLAVKLHSPIVLMDNSGLSSEQQRVFANKKVETPY
                     QIGGGVSYIVMDKLMDILAK"
     gene            complement(4313..5326)
                     /gene="galE"
                     /locus_tag="PC471_002867"
     CDS             complement(4313..5326)
                     /gene="galE"
                     /locus_tag="PC471_002867"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HDF2789979.1"
                     /translation="MAVLVAGGAGYIGSHTAIELLESGYEVVIVDNLSNSNSIVVDRI
                     KELSKKPVKFYNIDIRNKDEMHVVFKENNIESIIHFAALKAVGESVEKPIEYYSNNLI
                     STLNLFELMREYGVKKFVFSSSATVYGDPHTCPILEDFPLSVTNPYGRTKLMIEQMLV
                     DISKADKSLDIALLRYFNPVGAHKSGRIGEEPNGVPSNLMPYITKIAVGKLKELSVYG
                     NDYPTHDGTGVRDYIHVLDLAAGHVKALQKLEENPGLVVYNLGTGKGYSVLDLVKAFS
                     KASGKEIPYKIVGRRAGDVAMCYADSSKAEKELGWKAKYELEEMCEDSWRWQSMNPNG
                     YEE"
     gene            complement(5350..6219)
                     /gene="galU"
                     /locus_tag="PC471_002868"
     CDS             complement(5350..6219)
                     /gene="galU"
                     /locus_tag="PC471_002868"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HDF2789980.1"
                     /translation="MTVKKAIIPAAGLGTRFLPATKSQPKEMLPIVDKPTLQYIIEEA
                     IESGIEEILIITGRNKKSIEDHFDKSVELELELEQKGKTEMLEMVRDISNMVNIHYIR
                     QKEPKGLGHAIYCAKSFIGDEPFAVLLGDDIVDSDVPCLKQLIDTYNEYKTTVLGVQK
                     IAKEDTNKYGILDVKHIEDRVYKVKDMVEKPAIEEAPSDIAILGRYIITPAIFSILEK
                     QTPGKGGEIQLTDALQTLGKQEAIYAYNFEGRRYDVGDKIGFLEATIDFALKRENLKD
                     DLMDYMRKKVSEK"
     gene            complement(6417..6737)
                     /locus_tag="PC471_002869"
     CDS             complement(6417..6737)
                     /locus_tag="PC471_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2789981.1"
                     /translation="MSSKYNVWTFNYEFLGLDSGDEKNNEVYYEIDLDEILNENYIDE
                     DIDSGLDSDGNSILGELISEMDIDEDVSVDMTYQELEVDLEDLDSYLDEDIDSDIKGI
                     LDEI"
     gene            complement(6727..8718)
                     /locus_tag="PC471_002870"
     CDS             complement(6727..8718)
                     /locus_tag="PC471_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2789982.1"
                     /translation="MLKLGEKIIYELSDGFELSGISRYSKEYKKLHITNLGNIIISKS
                     DEVNEDTGVKYIYSFSDNKEKLEAVLEDSQIIVYGNNLPFYIKSENSSKNVTANLTMK
                     ITLDEYDLICKSQREFIFYLKDNMTILSLDDNKFYMGGINKDNEKFIFISGKNRFEIN
                     FDDIERYILEDKRVSLKGYFHMEREGIIVRSVSIFNNNIDSVVPSDLNERVKDNQKIG
                     NLPKDCEIVFCKISGNIDGFDYKNTNMLLVKYQDQLIFINKKSKKTIVKSAKDNCSKL
                     NLGEDIILYDNKNVFNLHINDKNREIMQIDDLKDIENEIVGYTLKHAPFFIQEDFDSL
                     TILKSFQKEIISIKNSDIKDIVINKELENENSNFVETEIKFNNQKVLLNLSKSMVQKL
                     MQDVFIYAKQPLLKENSIEVIYKNWSKAMNDMIIFNFFGNIYYMKSEFDKILEKELND
                     EIRIEVINSLYKQIQEQRNNLDLLSAYMPRILENQEIDLFEKYNTKLDVQVFKQIKNL
                     LSDLSYNISSYLNEVEKSLDNIIFVISGEDKKKYNYRMLKESESASLDVFLKQAISRL
                     NHLVENMYPYYVDETSREMFKLFELLWKNYRNIDDDSIKEILFERITNTYVFKQLTLN
                     NSTKERRKDIIEKIYNSVDYGTNKLDENMFFTGGIKYVK"
     gene            complement(8841..11384)
                     /locus_tag="PC471_002871"
     CDS             complement(8841..11384)
                     /locus_tag="PC471_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HDF2789983.1"
                     /translation="MKVLSENNIIRNTVLNVTASFLKQESKINEKLDGVLEKKFEKVE
                     FNEAKYAELLKFNILFYKTLARNTEPLIGKWIVDKYIPEIDELEKDLELTTAKCRKYV
                     NKAMKEGLDSLKANDLNSFLAYDKMELSERRRRLEKDYKVLNLYKDLLNISLRKISLE
                     KKECGDLFLKNQADAKRELKREIIFCVNKILASNKDVVPNNVEENNEQNKVEIKATEI
                     EDLQLGKDNSENDMLLNEIKNSKVKHESKSNDIESKFIDKLNSLNNESISQTIHNEYK
                     KLCGLEELHSVEAYGFGKEIIKDFACATVVLEFLKRRNRDLIEGAMRLTIIGEFGPEN
                     FKEFVDYVIKNKTEISEDTWNKAQNLIKDNYSELENHEIASKRTRRNKDIDIEEYIYM
                     IKNADKDICFRSSISIEDDAEEGTKEEVDSNNQDIGDVVEDKDTTDKEYDSNKEDIIE
                     PENKKSKKKAKLFGFIKKDNEEAEQEEENLNDISPDIILDKPVENHQVKSEEIEQNEL
                     KEIKQEEPSQHIEEERSVKIEKPINNNLDEKVSSNNESKLEKESKNLEDKKAKEIKEE
                     KLENEKSVVIPIKKKENSNKKSKNSSKDKYRENKKEMKNYVSDKEDSLDDEEVVSKKS
                     RLKETIIAVVIVVIVGVGYFITVGNNKKNDKENIPKSSTQQQANNKLTEEEKKAQAEK
                     EKKEAEEKAKAEKEAQEKAQAEKKAKEMEAYKDGKGVYYTVYAGSLKVEKTAKETAKE
                     YEAKGISSTIIQENGYYKIKIGDYSQYGEAQEKCNELAKKSIDTYIAMYDKYYDYKLE
                     ELKESAPSLSAEELKQKYEDLRSELKNKSGYREYVKHLDKLYEEIVEGA"
     gene            complement(11662..11996)
                     /gene="rnpB"
                     /locus_tag="PC471_002872"
     ncRNA           complement(11662..11996)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PC471_002872"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            12128..12805
                     /gene="srtB"
                     /locus_tag="PC471_002873"
     CDS             12128..12805
                     /gene="srtB"
                     /locus_tag="PC471_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sortase SrtB"
                     /protein_id="HDF2789984.1"
                     /translation="MKKLYRIVINIILVLVILYSGFNIYSKLTKYNHDTKISSELQKK
                     EYKKEDLSKINSDFKFWLSVENTNINYPVVQSKDNSYYLDKDFYKKDSISGTLFMDYR
                     NKSIDDKNIIIYGHNMKNKTMFNNLNKFKDADFFKKNNKIKITLNGKEFLYDVFSAYI
                     VESDYDYLKTNFNNESDYQNYINDITSKSLYKSPIKVNSNDKIVTLSTCTYEFDDARM
                     VIHGRLI"
     gene            complement(12965..13039)
                     /locus_tag="PC471_002874"
     tRNA            complement(12965..13039)
                     /locus_tag="PC471_002874"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13005..13007),aa:Thr,seq:tgt)
BASE COUNT         4034 a         2293 c         1333 g         5424 t
ORIGIN      
        1 taaatatatt tatcttataa ttcagacaaa aaacgataaa tttttttaaa agcttttatt
       61 cttactctat aatcatatat aataaaaatg taaaaatatc ttaaaggtta acttctacaa
      121 atatataaat aaaaatacct atgaaaggat gaacaaaaat tgaatttata tcacttacgc
      181 tattttgtta cattggcaca tttagaacac tatacaaaag cggcagaaaa tttatctata
      241 acacaaccaa gcttaagtca tgctatttct ttactagaaa atgaacttgg ggtggctcta
      301 tttgaaaaag aaggtagaaa tatagtcctt actaagtatg gaaaaatatt tttaaaagat
      361 gtggaaaaat cattagaaat tttagattca agtgtaaaat cattaaaaat aactggtact
      421 ggtgaaggac aaatagactt agcttttctt agaacacttg gtactgattt tatcccagac
      481 atagtgcaca aatttttaaa atctaatcct gctaaatcaa tagattttaa atttcatact
      541 ggtgtaacta ctgacattat tcaaggtctt aaagaaagaa aatatgatat tgcattttgt
      601 tcgaaacttg aaaaagaaaa gggaattgaa tttatacctg ttgccaaaca agatttagta
      661 ctaattgtac catatagtca tccactagct gcaaaggata ctatagattt aaaagaaacc
      721 ataccttatc ctcaaatagt atttaatcag agaagtggtc ttagatatat aattgatgat
      781 atgtttaaaa aaataaatca acagcctaat atagtctatg aagtagagga agaccaagta
      841 attgctggat tagttgcaaa aaatttcgga attgctgtag tcccaaatat gaatatgctt
      901 agctttacaa aagtaaaagt aattcagatt attcacccaa gttgggaaag aaacttttac
      961 ttagcattta taaaagatag atatttgcct cctgcaatta aaaactttaa aaactttgtc
     1021 attaaaaacg ctcaattata atttattaat aaaggggtta tttttaaata tcctctttta
     1081 ttctttttat aaaataaaaa aatctatact tttaaaagta tagattccta actgacagtc
     1141 ggcatccgag tctcagtaat tatcgttata caacagcaac tggattcagt tcctgaataa
     1201 ctaggttact tacattatat cagatttttt aaaatttgtc aattgtatct aacgcatttt
     1261 ttcaagccat ggtccaacag gctctacaat aggaacttct ttaaatcccc aatttcttat
     1321 atcattcaca ctcgttggag tatatatttt gtttctgaca gatttgaata ttttgttttt
     1381 ttctccatat tgcatacact ttggattagc ttctattaac cctcttcttg ctctgctaag
     1441 aggattacat aaaatatctg ctatttctaa accttctcta tagtcagcat cttctttttt
     1501 ggcaattata aaacccttaa ccttatctct taattcttca tctatatcta agtatattgt
     1561 tccattatta aatacatcaa aaaacgcctt ttgtactttt aagtttgaga catcatccct
     1621 tgactctata actattcgtg cagattctac agtttcatta tttacaaatg aataaaaatt
     1681 ctgaagaaca tgtataaaag ccacaacata cggttccttt gaaggtgcat aatatttctt
     1741 taatctagct ttatctactg ttactgatat tatagtacac tctaatctag atagaaataa
     1801 aggcaactca tcatagaata acttaatctg tgaatcatca aacttctcaa atgctcttga
     1861 ttttcttgat atatctgtca aatgtattcc tatgttactg gttccaaaac aatactcttt
     1921 aaatgcatcc atttctttcc tcaaagaaca tacataatcg ccttgactta ctgcatattt
     1981 atattcaaaa attactcctg tcaatgtaaa tgggtcttca tgatttatat cagctcttcc
     2041 actttcatca ataaacatta tatatttttt ctttataatc ttactcaccc tcttaacata
     2101 tgatgatagc atctatattc attttatatg agttttaaaa atattccaag atttttatat
     2161 aacttaaact taattttata ctaaaaaatt gagatgtaaa acaatcaagc ttttattgat
     2221 tgaatttcac atctcaatta taattatttt tcaatatata tttttactat tttgcaagaa
     2281 tatccattaa tttatccatt actatataag atactccgcc tcctatttga taaggagttt
     2341 caactttttt atttgcaaat actctttgtt gctctgatga taatcctgaa ttatccatta
     2401 aaactattgg tgaatgtaat tttacagcta atggtccagc tgataaagca tctactaaag
     2461 catctgattt actaactaat acagcctgta aatcagtatc tccatagaat ttttctatca
     2521 ctttggcatt agtatctact ctactatctc catatattct atttcctgaa atatcttcag
     2581 tagttatatc attcattttt gctattactg aatcgttaat tgcacttggt tcacctataa
     2641 agtatgcatt ttctaatttt ctttctgtta gccatttata agattcttct gtaattccat
     2701 ctttattgac taatattata ggttgttttt cttccccagc tttagaagct acagataaag
     2761 catctgcctc cccatttcca gaaaccatat atgcagtttt tatttcttct cgatttccaa
     2821 gttcttcagc tattcttgta gcagtttgat atctatcttg acctgctatt ctctttgttt
     2881 ctgtgttgaa agtagttttt atttcttgct ctaatttaga acttattgca ttttctccac
     2941 ctatcaagat tactgtttta ggtgctaata acttcatttc ttcaacagtt tcagttggta
     3001 aattagctgt attagtaagt agtattgttg agtcatagct acttgctaaa ggagttgctg
     3061 ttataccatc agctattgca ttaccattta ctaatattac agtatctgaa ctatcagcat
     3121 tccagtttcc aactgaactt atctttttag ctgtatcaaa tctatttgca cctataattg
     3181 tatcccatgt aggatttata gttccatctt cattaaaagt ataatctttc ccatctattt
     3241 tttgagaacc agttagcatc tttccatctt tgtctaaata gtaagactta ccatctattt
     3301 gttgccatcc tgtctgcatt attcctgttt tgtcaaagta atacttatta ccatctattt
     3361 cttgccatcc tgttactgct aaaccatcag atcctagata gtatttcttt ccattctttt
     3421 cttgccatcc tgtttgcatt actccatctg agttaaagta ataagttttt ccgtctatta
     3481 ctttccatcc agatactctt gctccatcag ttcctaaata atattttttg ttattaattg
     3541 ttttccatcc tgtttgcatt actcctgaag catcaaagta atacattttt ccatttactt
     3601 ttagccaact attcttttgg tatgttccat cagattttac ataatacttc ttaccatttt
     3661 ctgttttcca tcctgtttga tttagattta tcttatattt tgccgctatc tttacatagc
     3721 tgtcgttact ataatctata attacatctg aacctacagg aacttggtca aataaccatc
     3781 taacatcttt attgtgcatt cttatacatc caccagaaat atgctttcct atagaactct
     3841 cattattatt tccatgtatt ccatatgtat ctccataagt tccttttaat gctaatccca
     3901 tccatctatc tcctaatgga tttcttggac ttcctccagg tatattacct ttataatatg
     3961 gtctattttt tatcttgtta actacttttg ttttccctgt tggtgtttct gacccctttt
     4021 tacctgttgc aactctaaat tcttttacaa gtttattatt tacaaaatat cctagagtat
     4081 tttttcttga atttactata atcacatgtt tactggctgc gcttgattct ggtacgccac
     4141 atgcaaatac taaaactaaa gtcagtgcta gtactaaata cttttttaga ctttttttca
     4201 ctataacttc cccctctttt agaaacatta ctacatgtgt ctaattataa cataaaatta
     4261 ccaatacctt atatttacaa atttgttaca tttatacata taagtatgta ttctactctt
     4321 catacccatt aggattcata ctttgccatc tccatgaatc ttcacacatc tcttcaagtt
     4381 catacttagc tttccatcca agctcttttt ctgcctttga agaatctgca taacacatgg
     4441 ctacatctcc agcccttctt cctacaatct tatatggtat ttcttttcca cttgctttag
     4501 aaaatgcttt tactaaatca agaacactat acccctttcc agttcctaaa ttataaacaa
     4561 caagtcctgg attttcttct aatttttgta atgcttttac atgaccagca gctaaatcta
     4621 atacatgtat atagtctcta actccagttc catcatgtgt tggatagtca ttaccataaa
     4681 cactcaattc cttcaattta cctactgcta tttttgttat ataaggcatt agattgctcg
     4741 gaactccatt tggttcttca cctattctac cacttttatg agcaccgact ggattaaagt
     4801 atcttaaaag agctatatcc aatgacttat ctgcttttga tatatctact aacatctgct
     4861 ctatcataag tttagttctt ccatatggat tggttacaga aagtggaaaa tcctctaata
     4921 taggacaagt atgtgggtct ccatatacag tagctgaaga actaaataca aactttttaa
     4981 caccatattc tctcattaat tcaaataaat ttaaggtact tattagatta ttgctgtaat
     5041 attcaattgg tttttctact gattcaccaa ctgcttttaa agctgcaaag tgtataattg
     5101 attcaatatt attttcttta aatacaacat gcatttcatc tttatttctg atatcaatat
     5161 tataaaattt tactggtttt ttagataatt ccttgattct atcaaccact attgagttgc
     5221 tattacttaa gttatcaact ataacaactt cataacctga ttctaaaagt tctattgctg
     5281 tatggcttcc tatatatcct gctcctccag ctactaaaac tgccattgta attacctctt
     5341 ttctaatatt tatttttcac ttactttttt tctcatatag tccattaaat catcttttaa
     5401 attttctctc tttagagcaa agtctattgt tgcctctaaa aaaccaattt tatctcccac
     5461 atcatatctt ctaccttcaa agttataagc ataaatagcc tcttgttttc caagagtttg
     5521 aagtgcatct gtcagttgta tctctccacc tttaccaggt gtctgtttct ctaatatgct
     5581 aaatattgca ggagttatta tatatcttcc aagtatagct atatcagatg gagcttcttc
     5641 aattgcaggt ttttcaacca tatctttaac tttatatact ctatcttcga tatgttttac
     5701 atccaatata ccatatttat tagtatcttc tttagctatc ttttgaacac ctaatacagt
     5761 tgttttatac tcattataag tatctattaa ttgttttaag cacggaacat ctgaatctac
     5821 tatatcatca cctaataata cggcaaaagg ttcatcacct ataaaacttt ttgcacagta
     5881 aattgcatgt ccaagacctt ttggctcttt ttgtcttata taatgaatat ttaccatatt
     5941 tgatatgtct cttaccattt ctaacatctc tgtcttacct ttttgctcta gttctaactc
     6001 taattcaact gatttatcaa aatgatcttc tatagatttt ttatttctac ctgttattat
     6061 aagaatttct tcaattcccg attctatagc ttcttctatt atatactgta aagttggttt
     6121 atctactata ggaagcattt ctttgggctg tgattttgtt gctggtaaaa atctagttcc
     6181 tagccctgct gctggtatta ttgctttttt aactgtcatc tgcatttttt cacccttctt
     6241 cgtaaatttt ctattgtacc aaattaatat accatattta tactttttct tcaaactcta
     6301 tattatccca atatttaatg ctataagata atataaggta gatgaaattt catctacctt
     6361 tagttttata agttaatatt gtattgttta tattttatcc aaaacttata caggtactaa
     6421 atttcatcta agatgccttt tatatcacta tctatatctt catctagata agaatctaaa
     6481 tcttctaagt caacttctag ttcttgatat gtcatatcaa cactgacatc ctcatctata
     6541 tccatttctg atattaattc tcctaaaata ctatttccat cagaatctaa accactatct
     6601 atatcttcat ctatatagtt ttcatttaaa atttcatcta aatcaatctc gtaataaact
     6661 tcattatttt tttcatcccc tgagtccaat cctaagaact catagttaaa agtccaaaca
     6721 ttgtatttac ttgacatact ttattcctcc tgtgaaaaac atattttcat ctagtttatt
     6781 ggttccataa tcaacagagt tatatatttt ttcaattatg tcttttcttc tttcttttgt
     6841 actattattt aatgtcaatt gtttaaatac atatgtattt gttattctct cgaataatat
     6901 ttcttttata ctatcatcat caatatttct atagtttttc cacaataact caaataactt
     6961 aaacatttcc ctactagttt catctacata ataaggatac atattttcta ctaaatggtt
     7021 taatcttgat atggcttgtt ttaaaaatac atcaagactt gcactttcac tttcttttaa
     7081 cattctataa ttgtatttct tcttatcttc tcctgaaatt acaaagatga tattgtctaa
     7141 acttttttca acttcattta aataactgct tatattatat gataaatcag acaatagatt
     7201 tttaatctgc ttaaacactt gtacatcaag tttagtatta tacttttcaa acaaatctat
     7261 ttcttgattt tctaatattc ttggcatata tgctgacaat aaatctaaat tgtttctttg
     7321 ttcttgtatt tgcttataaa gtgaatttat aacttctatt cttatttcat catttaactc
     7381 tttttctaaa atcttatcaa attcagattt catatagtaa atatttccaa agaaattaaa
     7441 tattatcata tcattcatag ctttagacca attcttatat atcacttcaa tagaattttc
     7501 tttaagtaga ggttgctttg catatataaa tacatcttgc ataagttttt gaaccatact
     7561 tttacttaag tttaaaagta ctttttgatt attaaatttt atttccgttt ctacaaaatt
     7621 agaattttcg ttttcaagtt ctttattaat tacaatgtct tttatatcac tattttttat
     7681 agatatgatt tctttttgaa aagactttag aatagtcaag ctatcgaaat cttcttgaat
     7741 aaaaaatgga gcatgtttta atgtatatcc cactatttca ttttctatat cttttaggtc
     7801 atctatttgc ataatttctc tatttttatc atttatatgt aggttaaata catttttatt
     7861 atcatataat attatatctt ctcctaaatt taacttagaa caattatctt tcgctgactt
     7921 tacaatagtc ttcttagatt tcttatttat aaaaattaat tggtcttgat attttactaa
     7981 aagcatgttt gtgtttttat aatcaaatcc atctatattt cctgatatct tgcagaatac
     8041 tatttcacaa tctttaggaa gatttcctat tttttggtta tctttaactc tctcatttaa
     8101 atcagatggg actacactat ctatattgtt attaaatata gatacagacc ttactataat
     8161 cccctctctt tccatatgaa agtatccctt caatgacact cttttatctt ctaaaatgta
     8221 tctttctata tcatcaaaat ttatttcaaa tctatttttc ccactgataa atataaactt
     8281 ctcattatct ttattaattc cacccatata aaacttattg tcatctaaac ttaatatagt
     8341 catattatcc tttagataga atataaattc tctttgactt ttgcagatta aatcatattc
     8401 atccaaagtt attttcatag tcaaatttgc tgtaacattt ttacttgaat tttcactttt
     8461 tatgtaaaaa ggcaagttat ttccatagac aattatttga gaatcttcta aaactgcctc
     8521 taatttttct ttattgtctg aaaaactata tatatatttt acacctgtat cctcattaac
     8581 ttcatctgat ttgctgatta ttatattccc tagatttgtt atatgaagct ttttatattc
     8641 ttttgagtat ctacttatgc cactaagctc aaacccatct gaaagctcat atatgatttt
     8701 ttctcctaat ttcaacatac cttcaccacc taaacagaat tataagtatt ttataaaggt
     8761 gaataaaatg tctcaaaaca aataatattt tttttaatac tttttattct gagacatagt
     8821 ttctcctttt ttataatgct ctaagcacct tcaactattt cttcataaag tttatctaaa
     8881 tgtttaacat attcacgata tccagatttg ttctttaact cacttcttaa atcttcatat
     8941 ttttgtttaa gttcttcagc actcaaagaa ggagctgatt cttttaattc ttctaactta
     9001 taatcatagt acttatcata catagctata tatgtatcaa tagacttctt agctagttca
     9061 ttacattttt cttgtgcttc tccatattga gaataatcac ctatttttat tttatagtat
     9121 ccattttctt gaattattgt tgaagaaata cctttagctt catattcttt tgctgtttct
     9181 tttgctgttt tttcaacttt aagtgaacct gcataaacag tgtaatatac acctttacca
     9241 tctttatatg cttccatttc ttttgctttc ttttcagctt gtgctttttc ttgagcctct
     9301 ttttcagctt ttgccttttc ttctgcttct tttttctctt tttcagcttg tgcttttttc
     9361 tcttcttcag ttaatttatt gttagcttgt tgttgagttg atgactttgg aatattctct
     9421 ttatcatttt tcttattgtt gcctactgtt ataaaatatc ctacaccaac aataaccact
     9481 ataactactg ctataatagt ttcttttaat ctactcttct tagaaacaac ctcttcatca
     9541 tctaaagaat cttctttatc tgatacataa tttttcattt ctttcttgtt ttctctatat
     9601 ttgtccttag aagaattttt agatttttta ttactatttt ctttcttttt aataggtatt
     9661 actactgatt tttcattttc taatttttct tcttttattt cttttgcttt tttatcttct
     9721 aagtttttac tttctttttc aagcttcgat tcattatttg aagatacttt ttcatctaag
     9781 ttattattta ttggtttttc tatttttaca ctacgttctt cttctatatg ttgacttggt
     9841 tcttcttgtt ttatttcttt caattcattt tgctctattt cttctgattt cacttgatga
     9901 ttttcaactg gtttatctaa aattatatct ggagaaatat catttaaatt ctcttcttct
     9961 tgttcagctt cttcattgtc ttttttaata aatccaaata atttagcctt ctttttactc
    10021 tttttatttt ctggttcaat tatatcttct ttattgctgt cgtactcttt atctgtagta
    10081 tctttatctt ctacaacgtc acctatatct tgattattac tatctacttc ttctttagtc
    10141 ccttcttcag catcatcttc tattgaaata ctacttctaa agcaaatatc tttatctgca
    10201 ttttttatca tgtaaatgta ttcttcaata tcgatatctt tatttcttct agttcttttt
    10261 gatgcaattt catgattttc taattcacta tagttatctt ttattagatt ctgtgcttta
    10321 ttccatgtat cttcactaat ttctgtttta tttttaatta catagtccac aaattcctta
    10381 aaattttcag gaccaaattc tccaattata gtcagtctca ttgcaccttc tataaggtct
    10441 ctatttcttc tctttaaaaa ttctaataca actgttgcac aagcaaaatc tttaataatt
    10501 tctttgccaa aaccatatgc ttcaacagaa tgaagttctt ctaatccaca taacttctta
    10561 tattcattgt gtattgtttg agatatactt tcattattta agctatttaa cttatctata
    10621 aacttcgatt ctatatcatt tgattttgat tcatgtttta cttttgaatt tttaatttca
    10681 tttagtagca tgtcattttc tgaattatct ttacctaatt gcaaatcctc aatttcagtt
    10741 gcttttattt ctactttgtt ttgttcgtta ttctcttcca cattattagg taccacatct
    10801 ttattactag ctaatatttt gtttacacag aatataattt ctctttttaa ttctcttttc
    10861 gcatctgctt gatttttaag aaataaatct ccacattctt tcttttctaa acttattttt
    10921 ctaagagata tattaagaag gtctttatat aggtttaaaa ccttataatc cttttctaaa
    10981 cgtcttcttc tctctgatag ttccatttta tcataagcta aaaaactatt taaatcattg
    11041 gcttttagag agtctaatcc ctctttcata gccttattta catactttct acattttgct
    11101 gtagttaact ctaaatcttt ttctaattca tctatttcag gtatgtactt atctactatc
    11161 catttgccaa taagtggttc tgtatttctt gctaatgtct tataaaacaa tatattaaat
    11221 tttaaaagct ctgcatattt tgcttcatta aattctactt tttcaaattt cttttccaaa
    11281 acaccatcta gcttttcatt tattttagac tcttgtttta aaaaacttgc tgtgacattt
    11341 aaaacagtgt ttcttattat attattttca ctcaaaacct tcactcctta aatcaattat
    11401 tttattgtaa tatttacatt gtgcaaagtt gctaatttga ttatgatatc taaataatta
    11461 tttactaaat ttgctacatc ttatatcata tcatattatt tacatcattt tactttttct
    11521 ttttatatct atcttatatt ataccatttt taatatctgt actaacttat attacaatat
    11581 tcctcaaaat tttaaaagta gattagacaa atttacaaat aaaaaaacat cagtttaaac
    11641 tgatgttttt gactatcgaa ataattaagc aagtctataa gcggagttct gtattagata
    11701 atcatctttc taggcctatt gtcaccaata ggctcaagcg acctacctac cggagagtac
    11761 gagcagcact ctttattcct atcttggtct tgctccaggt ggggtttaca tagccaacta
    11821 gtcgcctagc tgctggtgag ctcttacctc gcctttccat ccttaccaca atagtggcgg
    11881 tttatttctg ttgcactatc cttaggatcg ctcccactag gcgttaccta gcaccctgct
    11941 ctatggagct ccgactttcc tcatgtaaga ttacatgcga ttatctggct tacttaatat
    12001 ttattttact tagttatgtt aacacaaatt cttttacatg tcaaagtaat attatactaa
    12061 aaatttaaat ttattcaaaa atagtgtaat atagatatat aaactacatt taactcgagg
    12121 tgatctattg aaaaaattat atagaatagt aattaacatt atactagtat tagtaatact
    12181 ttacagtgga tttaatatat actcaaaact taccaaatac aatcatgata ctaaaatatc
    12241 ctcagaactt caaaaaaaag aatataaaaa agaggattta tcaaagatta acagtgattt
    12301 taaattttgg ctttctgttg agaatacaaa tataaattat ccagttgtac aatctaaaga
    12361 taattcttac tatcttgaca aagattttta taaaaaagat tctatttctg gtacactgtt
    12421 tatggattat agaaataaat ctattgatga caaaaatatc ataatatatg gacataatat
    12481 gaaaaataaa actatgttca acaacctaaa taagtttaaa gatgctgact tctttaaaaa
    12541 aaataataaa ataaaaataa ccttgaatgg aaaagaattt ttatatgatg ttttttctgc
    12601 ttatattgta gaatctgatt atgattatct aaaaacaaat tttaacaatg aatctgatta
    12661 tcaaaattac ataaatgata ttacttctaa atcattgtat aaatctccta ttaaggtaaa
    12721 tagcaatgat aaaatagtta cactttctac atgtacctat gaatttgatg atgccagaat
    12781 ggtgattcat ggtagattga tttaaaactc acagacacag tttaaaacaa taattttaaa
    12841 ctgtgtcatc ttttatacct ataatttgta aattagacca ctaaaagtat ttaatacaaa
    12901 ataacaaata catcaaacaa taaaaaacaa taaaaaaaga ctatgctaaa aacatagtct
    12961 ttactggagc tggtgatagg aatcgaacct acaacctgct gattacaagt cagctgctct
    13021 accgttgagc cacaccagca cacctatttt attgtaaatt agcaaaacta atttttaaaa
    13081 gtta
//
LOCUS       DAMJYR010000106        13053 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.109, whole genome shotgun sequence.
ACCESSION   DAMJYR010000106 DAMJYR010000000
VERSION     DAMJYR010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13053)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13053)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13053
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.109"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(<1..1185)
                     /locus_tag="PC471_002875"
     CDS             complement(<1..1185)
                     /locus_tag="PC471_002875"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000469013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HDF2789985.1"
                     /translation="MFNTIEEAIEDIKNGKMVIVVDDESRENEGDLLMAAEMATPESI
                     NFMATYGRGLICLPATEKKFKSLNIPLMVRENTDTFQTAFTVTIDGADTLTGISAYER
                     AETVKLFCDENSTSKDFKTPGHIFPLIAKTGGVLVRDGHTEASVDLARLAGFKEIGLI
                     CEIMKDDGTMARVDDLMIFKEKHNLKIITIKDLIEYRKINETTIEKVSSAFLPTKYGN
                     FEIIGYRDTYSNEEHIALTYGNINIENTLVRLHSECLTGDVFHSLKCDCGLQLESSMK
                     KIVENGSGVLIYMKQEGRGIGLLNKIKAYKLQEEGYDTVEANLMLGFEEDMRDFYMAA
                     QILKNLNIKSINLLSNNPDKINQLEKYGIKIENRIPINEEINDFNKLYLKTKKDKMGH
                     LLD"
     gene            complement(1208..1873)
                     /locus_tag="PC471_002876"
     CDS             complement(1208..1873)
                     /locus_tag="PC471_002876"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HDF2789986.1"
                     /translation="MFTGIVEEVGILRKITANGKSGKVTILSNKILDGTNLGDSIAVN
                     GVCLTVSNLGKNEFTADVMMETIRSTNLGLLNANDKVNLERAMSLSSRFGGHIVTGHV
                     DGKGTICKFEKDENAVLVSIRPDKKLLSSMILKGSVAIDGVSLTISYLDDEIFKVSII
                     PHTKINTILLTKNVGDFVNLESDVIGKYVNNFMANNYKELNSNSNNHKSNIDKDFLFK
                     NGF"
     gene            complement(1907..3019)
                     /gene="ribD"
                     /locus_tag="PC471_002877"
     CDS             complement(1907..3019)
                     /gene="ribD"
                     /locus_tag="PC471_002877"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HDF2789987.1"
                     /translation="MNFVNQKEKDIYYMKKAIELAKNGEGFVNPNPLVGCVIVKASNI
                     IGKGYHEKFGSNHAEVNAINSAKQSLKDSTLYVNLEPCSHYGKTPPCVDKIIQNKIKR
                     VVISTLDPNPLVCGNGVKKLKDNNIDVTVGILEDEARDLNEAFFYHIKNKRPLCIVKS
                     AVSLDGKIATKSLESKWISNESSRYLTHKYRNKYQSIMVGINTVLNDNPLLTCRLNQE
                     KVSHPTRIVIDTHLKLPLNSNLVKDKTSKTIVFTCCKESIKLSMLKENNVETIISPSK
                     NNLVDLEFVMYKLGELNIDSVLVEGGATLNDSLFRNKLVDKVKLFLSPKIIGGKDAPT
                     FVSGEGINHLSDSTQLTINNVTLIDGDILIESDVLN"
     regulatory      complement(3151..3272)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            3461..5944
                     /gene="recQ"
                     /locus_tag="PC471_002878"
     CDS             3461..5944
                     /gene="recQ"
                     /locus_tag="PC471_002878"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HDF2789988.1"
                     /translation="MNKKLLEILSKYYGYTSFRKGQESIINSILSEKDVLAIMPTGGG
                     KSICYQIPALILDGMTIVISPLISLMKDQVDALKTMGISAAFINSSLSSKEFNEILNN
                     IRKNNYKILYIAPERLDAQEFIELINNNNVSQVAIDEAHCVSQWGHDFRLSYRRISDF
                     IKNLPKRPIVTAFTATASEEVRTDIINLLCLENPDYYITGFDRENLSINIVKSSSKNK
                     YILDYIQNHKNESGIIYVATRKEVENIYNGLSKRNISVSKYHAGLSKNERSKNQEDFI
                     NDNVDIMVATNAFGMGIDKPNIRWVIHYNMPQSIENYYQEIGRAGRDGEKSECVLLFS
                     PGDVHIQKYLIDIGVENPERKLFQHKKLQYMIDLVYSNSCYRKTILSYFGENYIDNCN
                     NCSNCLVEGEIVDKTIDAQKVISCVARMKRSYGVTTIVDVLRGSKNKKILQLGLNTLS
                     TYNIMRDYSSEDLKNFVNTLVSHGFLDLVETLGNGNRGSFPTIRLNEMSMKVLKGEVK
                     VEFKEIVMTKSLEVEDELYNALRELRHSIASEERIAPYMVFGDGTLRAMSSSYPINKE
                     EMLDISGVGEIKYQKYGRDFEAVIKEYVEKNNINKKIDEKNVDSKNVNSDFLEVNTDK
                     VLYEKLLVIREEYATKEKLPAYMILANKALKEISGRYPLDEEQLKDISGIGGVKFEKY
                     GASILAEVNKYVKENNINVIWEKKGKRKLILDGESRKNNEIALDLLNQGKDIQSISQE
                     IEVSLSTIIGYVHDYVKDGHSISFELGLENFYNEDKKKMILEVCEKVGYDNLNNIKRK
                     LPDSIKYENIRAVILDSYLENLKNNIIAE"
     regulatory      6033..6144
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            6286..7587
                     /gene="thiC"
                     /locus_tag="PC471_002879"
     CDS             6286..7587
                     /gene="thiC"
                     /locus_tag="PC471_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HDF2789989.1"
                     /translation="MNYTTQMDAARKGIITKEMEIVSQKEQVDVNELRELIANGQVVI
                     PANKNHKSLSAEGVGKNLRTKINVNLGISRDCKDIEKELEKVRVAIDMKAEAIMDLSN
                     YGKTREFREKVVEMSPAMIGSVPMYDAVGYLEKELKDITEEEFLNVIRQHAIDGVDFI
                     TIHAGLTRSVCQKIKNHERLTHIVSRGGSLLFAWMELNNKENPIYTNFDKILDICEEY
                     DVTLSLGDACRPGCIKDSTDGVQIQELVVLGELTKRAWERNVQVMIEGPGHMAIDEIE
                     ANVVLEKRLCHGAPFYVLGPLVTDIAPGYDHITSAIGGALACAKGVDFLCYVTPAEHL
                     RLPNLDDMKEGIIAAKIAAHAGDIAKNVKGAREWDNKMSKARADLDWCEMFRLAIDPE
                     KAKRYRDESTPTHEDSCTMCGKMCSMRTVKKILNNEELNLI"
     gene            7641..7835
                     /gene="thiS"
                     /locus_tag="PC471_002880"
     CDS             7641..7835
                     /gene="thiS"
                     /locus_tag="PC471_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HDF2789990.1"
                     /translation="MKVNGKEIEFEKDLTVIDLLNKYNLKSDRVVVEVNLEIIEESNY
                     NTYVLKDEDIVELISFIGGG"
     gene            7828..8631
                     /gene="thiF"
                     /locus_tag="PC471_002881"
     CDS             7828..8631
                     /gene="thiF"
                     /locus_tag="PC471_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine biosynthesis protein ThiF"
                     /protein_id="HDF2789991.1"
                     /translation="MVKIKIFVNELLTDVEENTTAYKVRDSYNNKCDVLVLNGYPIKT
                     DMTLCENDKLTLIQKGVKPSLDELESLLIARHTPNIHNKLKKGKVAILGLGGLGSNIA
                     ISLARIGVGELLLIDYDVVEPSNLNRQQYFIDDIGKLKTDAMIENIKKINPFIKLNKR
                     DIFLNKNNMDTIKDSDLIIEAFDDASCKAQVCNYVLINLKDKYLIASSGMAGYYDSNI
                     ITTKKIKDRFYICGDFVNEAKFGEGLMAPRVAICANHMANLATQILIDM"
     gene            8694..9464
                     /locus_tag="PC471_002882"
     CDS             8694..9464
                     /locus_tag="PC471_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018592874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HDF2789992.1"
                     /translation="MDKLVLGGHEFNSRLLVGTGKYGSNNILPEVIKESGSEIITMAL
                     RRVDLDNKQENILTYIPKEMTILPNTSGATNAEEAVRIARISRKMGCGDFIKIEVISD
                     TRYLLPDNEETIKATKILADEGFIVLPYMTPDLYAGRRLIEANAAAVMPLGAPIGSNR
                     GLQMKEMIRIMIDELDIPIIVDAGIGKPSQAMEAMEMGADAVLVNTAIASAGDPVQMA
                     RAFKLAVEGGREAYIAKTGNVSEFANASSPLTGFLGNL"
     gene            9481..10584
                     /gene="thiH"
                     /locus_tag="PC471_002883"
     CDS             9481..10584
                     /gene="thiH"
                     /locus_tag="PC471_002883"
                     /EC_number="4.1.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HDF2789993.1"
                     /translation="MSFYDVIEKYRDFDFDGYLNNVTDNDVLRSLSKDKLEDFDILNL
                     LSKTAVKHLEDMAQKAHKLSVQYFGKTVCLYTPMYIANYCVNQCVYCSYNIKSGIKRK
                     KLTMDEIREEGEVISKEGFKHLLVLTGESSFHSSVEYIGEAIEILREKFPSIGIEVYP
                     MEVEEYKYIVDKGVEGLTVYQETYDEEIYKRVHIKGPKSNYKFRLDAPERGAKAGMRT
                     LSIGALLGLNDFRKETFFTILHGKYLKTKYPHIELSYSTPRMRPFKGCFEELVDISDT
                     DLVQAMVCMRLFDPHAAINISTRENLEMRSHIIPLGVTKLSAGVSTDVGGHSQDEHDT
                     AQFKINDESTVKDVEKMLNSIGYQHVFKDWERF"
     gene            10585..11274
                     /locus_tag="PC471_002884"
     CDS             10585..11274
                     /locus_tag="PC471_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HDF2789994.1"
                     /translation="MYLITNRKLCSEERYLEVIKESILSGVENIIIREKDLEYQELRK
                     LYMKIKTKINCIDFQEQISDESLKTNINQKEFRNKFKVNFIINSNIEFFEKVDCQGIH
                     LPFKLFLNLIENKYNFNENKILGLSLHKVEEVDYLEKLIRNQNIKIDYITLSHIYETK
                     CKEGLNPKGIELLKEAKKITDIKIIALGGILPSNVKETLKYCDDFAIMSTIMRSKDIK
                     KTISNYNEKLN"
     gene            11462..12856
                     /gene="yfcC"
                     /locus_tag="PC471_002885"
     CDS             11462..12856
                     /gene="yfcC"
                     /locus_tag="PC471_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HDF2789995.1"
                     /translation="MTDSKKKRFIIPHTFTIIFLLIVLMAILTWIVPSGEFSREDVEG
                     RMVVVPGTYQSVESNPQGFADVFKAPIEGFIDSAEVVGFVLIVGGAFGIVNRTGAIEA
                     GIASVISKFKGKELLIIPISMILFGLGGTTFGMAEETLPFYMIFVPLMTSLGYDSLTA
                     IAIVFIGAAAGAAASTINPFSVGIAQALAQLAPGSGVTYRVVIFIAYILISIAFVMYY
                     AKKVKENPESSIVYEIDKNNKSILNQTSTEIKEFTFKEVSVLAIFGIGMAIMIWGVLS
                     QGWYIPEISMCFAAIGVLSGLVGRLSQIEISESFIDGAKDLASAALAIALARGIVIIA
                     QNGFIIDTILNSAAAFLGGLPKVIFVYLSFFLEAALAFLIPSSSGLASLTVPVLAPLG
                     DLVNISAQVIVTAYQFGVGLTNFITPTSGVLMGALAVAHIPFSKWVRFIMPILLILTA
                     VSLVFLTIGIYIGF"
BASE COUNT         4897 a         1563 c         2223 g         4370 t
ORIGIN      
        1 atctaaaaga tgtcccattt tatctttttt agtttttaaa tatagcttat taaagtcatt
       61 aatttcttca ttaataggga ttctattttc gatttttatt ccatatttct ctaattgatt
      121 tattttatct gggttattac ttaaaagatt tatacttttt atgtttaaat tttttaatat
      181 ttgagcagcc atatagaaat ctctcatatc ttcttcaaat ccaagcatta aatttgcttc
      241 aacagtatcg taaccttctt cttgaagttt ataagcctta attttattta aaagaccaat
      301 acctctacct tcttgcttca tataaattaa aactccactt ccattttcca caattttctt
      361 catagaagat tctagctgta atccacaatc acattttaga gagtggaaca catctcctgt
      421 taaacattca gaatgaagtc taactagagt attttctata tttatatttc cataagtaag
      481 ggcaatgtgt tcttcatttg aataagtatc tctatatcct atgatttcaa aatttccata
      541 ttttgtcgga aggaaagcac tagatacttt ttctatagta gtttcattta ttttgcggta
      601 ttctataagg tcttttatag tgatgatttt taaattatgc ttttctttga aaatcattaa
      661 atcatcaact cttgccattg taccatcatc tttcattatt tcacagataa gaccaatctc
      721 tttaaaacct gcaagtctag caaggtctac tgaggcttct gtatgtccat ccctgacaag
      781 aaccccacca gtttttgcta ttaaaggaaa gatgtgacca ggcgttttga aatctttact
      841 tgtagaattt tcatcacaga ataattttac agtttcagct ctttcatatg cagatatacc
      901 agttagagta tctgctccat ctatagtaac agtaaaggct gtttgaaatg tatctgtatt
      961 ttctctaacc ataagaggta tattaagact tttaaatttt ttttcagtgg caggtagaca
     1021 aattagacct ctaccatatg tagccataaa atttattgat tcaggagtag ccatttcagc
     1081 tgccatcaat aaatctcctt cattctctct actttcatca tcaaccacga ttaccatttt
     1141 cccatttttt atatcttcaa tagcttcttc aatagtatta aacatgtaat tcactccttt
     1201 attattttta aaaaccattt ttaaataaaa aatctttatc tatatttgat ttatggttat
     1261 tactattaga atttaattcc ttgtagttat tagccataaa attattaaca tactttccaa
     1321 ttacatcgct ttctaaatta acgaaatctc caacattctt tgtcaagaga atagtgttaa
     1381 ttttagtatg aggtataata gaaaccttaa atatttcatc atctaaatat gatatagtta
     1441 aacttacacc atcaatagca acagaacctt tgagtatcat tgaggataaa agttttttat
     1501 caggtcgtat acttactaaa actgcatttt catctttttc aaatttgcag atagttcctt
     1561 taccatcaac atgacctgtt actatatgac caccaaatct tgaagaaaga ctcatagctc
     1621 tttcaagatt taccttgtca tttgcattta ataaacctaa atttgtggaa cgaattgtct
     1681 ccatcataac atcggctgta aattcatttt ttccgaggtt tgaaacagtt agacaaactc
     1741 catttacggc tatactatct ccaagatttg tgccatctaa tattttattt gatagtatgg
     1801 ttacttttcc actcttacca ttagcagtaa tttttctaag tattccaact tcttctacta
     1861 tgccagtaaa catcctaacc tcctatatga atgtattaac aaaaatttaa tttaaaacat
     1921 ctgattctat aagaatatcg ccatctatca aagttacatt attgattgtt aactgtgttg
     1981 aatctgacaa atgatttatt ccttctccag aaacgaatgt aggtgcatct ttaccaccta
     2041 taatcttggg tgatagaaat aatttgacct tatcaactaa cttatttcta aagagactgt
     2101 cattgagagt agcaccacct tcaactagaa cagaatcaat gtttaactct cctaatttat
     2161 acattacaaa ctccaaatct actaagttgt tttttgaagg agaaattatt gtttcaacat
     2221 tattttcttt taacatagat aattttatac tttctttgca acatgtgaaa actatagttt
     2281 tactggtttt atctttaacc aaattactat taagtggcaa ctttaggtgt gtatctatta
     2341 ctattcttgt aggatgagac actttttctt gattgagcct acaagttagt aaaggattat
     2401 cgttcaaaac tgtatttatg ccaaccatta tactttggta tttatttctg tatttatgag
     2461 ttagatacct tgatgattca ttagatatcc attttgattc tagtgatttt gtagcaattt
     2521 taccatctag acttactgct gattttacaa tacaaagagg tcttttgttt tttatatgat
     2581 aaaaaaatgc ctcatttaaa tctcttgctt catcttctaa tattcccaca gtaacatcaa
     2641 tattgttatc ttttaatttt tttactccat taccacaaac aagtggattt gggtctaatg
     2701 tagatattac aactctctta atcttatttt gtataatttt atctacacaa ggaggcgttt
     2761 tcccataatg agaacatggt tctaagttta cataaagagt agagtctttt agactttgtt
     2821 tagcactgtt tatagcattt acttcagcat gattagagcc aaatttctca tgatatcctt
     2881 tgcctattat attactagct ttaacaatta cacacccaac taatggattt ggatttacaa
     2941 agccttctcc atttttagcc aattcaatag cttttttcat ataataaata tctttttctt
     3001 tttgattcac aaaattcaca atatgtacac ctcctgttta attggataat ttttaaacaa
     3061 aaaaccctga gaattaactc agggtttaga ataatctaca ataaatgttt aataaatgta
     3121 gaacaaaatt gctctacaaa aacactcatt aaatatcttc ttccatccag actttactgt
     3181 cggctttgga atttaaccaa atcaaccaag ttttaccttg gctcgcgggc tataccgccg
     3241 attgggaatt tcaccctacc ccgaagatta agtattaaat ttatatttag aatgtatcat
     3301 aaattatttt actttgcaaa ctattttttt aaaaaatata tgttaatata taatatagaa
     3361 tgtctataaa ctttgaaata ccacgattgt gaatataaaa ataaaaattt gaacataatc
     3421 aggaaagaag tctaattaat ttaaattagg agagtaatat atgaataaaa aacttttaga
     3481 aatactatca aaatactatg gatatacaag ttttagaaaa ggtcaagaat ctataataaa
     3541 ctctatctta agcgaaaaag atgtgttagc tattatgcca acaggagggg gaaaatcaat
     3601 atgttatcaa attccagctt tgattttaga tggaatgaca atagtaattt ctccacttat
     3661 atcacttatg aaagaccaag tagatgcctt aaagactatg ggaattagtg ctgcttttat
     3721 aaatagttca ttaagtagca aagaatttaa tgaaatatta aataatatta gaaaaaataa
     3781 ttataaaata ctctatattg ctccagagag attagatgca caagagttta ttgagctaat
     3841 aaataataac aatgtaagtc aagttgcgat agatgaagca cattgtgtat ctcaatgggg
     3901 gcatgatttt aggttgagtt atagaaggat ttctgatttt ataaaaaatt tgcctaaaag
     3961 acctatagtt acagcattta ctgcaacagc ttcagaagag gtaagaactg atataataaa
     4021 tttactgtgt ttagaaaatc cagattatta tataactgga tttgatagag agaatttatc
     4081 aataaatata gtaaaatcat ctagtaaaaa taagtatatt ttagattata ttcaaaatca
     4141 taaaaatgag tctggaatta tatatgttgc aactagaaag gaagttgaaa atatatataa
     4201 tggtttatct aagagaaata tatctgtgtc aaaatatcat gcaggtttat ctaaaaatga
     4261 aagaagtaaa aatcaagaag attttataaa tgataatgtg gatataatgg tagcaacaaa
     4321 tgcatttgga atgggtattg ataaacctaa tataagatgg gttatacatt acaatatgcc
     4381 tcaaagtata gaaaactact atcaagaaat aggtagagct ggtagggatg gagaaaaaag
     4441 tgagtgtgta cttttatttt ctccaggaga tgtacatata caaaaatatt taattgatat
     4501 tggagttgaa aatcctgaaa gaaaattatt tcagcataag aagcttcaat acatgattga
     4561 cttagtttac agtaattcat gctatagaaa gacaatattg agttattttg gagaaaatta
     4621 tatagacaat tgcaataatt gtagcaattg tttagtagag ggtgaaatag tagataaaac
     4681 tatagatgca caaaaagtca tttcatgtgt agctagaatg aaaagaagtt atggtgttac
     4741 tactattgta gatgtgctta gaggttctaa gaataaaaag attttacagc ttggattaaa
     4801 tactttgtct acatataaca ttatgagaga ttattctagt gaagacttaa agaattttgt
     4861 aaatacattg gtttctcatg gttttttaga tttagttgag actctaggca atggaaatag
     4921 aggtagtttt cctactataa gattaaatga gatgtctatg aaagttttaa aaggtgaagt
     4981 gaaagtagag tttaaagaaa ttgttatgac aaaatcattg gaagtagagg atgagttata
     5041 taatgcttta agagaactta gacattcaat tgcaagtgaa gaaaggatag ctccatatat
     5101 ggtatttgga gatggtacac ttagagccat gtctagtagt tatccaatta ataaagaaga
     5161 aatgctagat atctctggag taggagaaat taaatatcaa aaatatggta gagattttga
     5221 agcagtaatt aaagaatatg tcgaaaaaaa taatataaat aaaaaaatag atgaaaaaaa
     5281 tgtcgattca aagaacgtta atagtgattt cttagaggtt aatacagata aagttttata
     5341 tgaaaaactg ttagtcatta gagaagaata tgcaacaaag gaaaaattac ctgcatatat
     5401 gattttagcc aataaggctt tgaaagaaat aagtggaaga tatccacttg atgaagaaca
     5461 attaaaagat atttctggca taggtggagt aaaatttgaa aaatatggag ctagtatttt
     5521 ggcagaggtt aataaatatg taaaagaaaa taatattaat gttatttggg agaaaaaggg
     5581 caaaaggaag ttaatattag atggagaaag tagaaaaaat aatgaaatag cattagattt
     5641 gctaaatcaa ggaaaagata tacaaagtat cagtcaagaa atagaagttt cactgtcaac
     5701 cattattgga tatgtgcatg actatgtaaa ggatggacat agtatatcat ttgaattagg
     5761 actagaaaat ttttataatg aagataaaaa gaagatgatt ttagaagttt gcgaaaaagt
     5821 tggctatgat aatttaaata acataaaaag aaaacttcca gattcaataa agtatgaaaa
     5881 tataagagct gtgatacttg actcttatct tgaaaactta aaaaataata ttatagcaga
     5941 gtaattttta acttaataaa aaataaaatt tgtattattg tgttgaacta ataattttgc
     6001 tgtgttatac tgaatttgta aaaataatga aaaattagag ctaggggcgc tagaattggc
     6061 tagctgagag ataaagccgt aacttacaaa ccctaataac ctgatcaaga taatgcttgc
     6121 gaagggaagc tttttaatat aattttatta tataaatagg caaattattc aatgttagga
     6181 gaaatttgtc taagtgtata tacgattata agagtgcttt ccaaacgagg aaaagcactt
     6241 tttttatttt caaaaactat aactttaatc ttggaggatt gtaatatgaa ctatacaaca
     6301 caaatggatg ctgctagaaa aggcataata actaaagaaa tggaaatcgt ttctcaaaaa
     6361 gaacaggtag atgtaaatga attaagagaa ttaatagcaa atggacaagt tgttatacca
     6421 gctaataaaa atcataaatc tttaagtgca gaaggtgtag gtaaaaattt aagaacaaaa
     6481 ataaatgtaa atctaggaat ttcaagagat tgtaaggata tagaaaaaga acttgaaaaa
     6541 gttagagtag caatagacat gaaagctgaa gctataatgg atttaagtaa ttatggcaaa
     6601 actagagaat ttagagaaaa ggttgttgaa atgtcacctg caatgatagg ttcagtacct
     6661 atgtatgatg cagtaggata tttggaaaaa gaattaaaag atataacaga agaagagttt
     6721 ttaaatgtaa taagacaaca tgctattgat ggagtagact ttattactat acatgcaggt
     6781 cttacaagaa gtgtttgcca aaaaataaaa aatcatgaga gattgactca tatagtatct
     6841 cgtggaggtt ctttattgtt tgcatggatg gaattaaata ataaggaaaa tcctatttat
     6901 actaattttg ataaaatatt ggatatatgt gaagaatatg atgttacatt gagtttagga
     6961 gatgcttgta gaccaggttg tataaaagat tctactgatg gtgttcaaat tcaagaactt
     7021 gtagtattag gtgaacttac taaaagagca tgggaaagaa atgtacaagt aatgatagaa
     7081 ggtcctggac acatggctat tgatgaaata gaagcaaatg tagtattaga aaagagatta
     7141 tgccatggag caccttttta tgttcttgga ccattggtta cagatatagc accaggttat
     7201 gaccatataa caagtgcaat aggaggagct ttagcatgtg ccaaaggagt agatttttta
     7261 tgttatgtaa ctccagcaga acatttaaga ttgcctaatt tagatgatat gaaagagggt
     7321 ataatagctg caaagatagc tgctcatgca ggagatatag ctaaaaatgt taaaggtgct
     7381 cgtgaatggg ataataagat gagtaaagct agagctgatt tagactggtg tgagatgttt
     7441 agacttgcaa tagaccctga aaaagctaag agatacagag atgaatcaac tcctactcat
     7501 gaggatagtt gtactatgtg tggaaaaatg tgctcaatga gaacagttaa gaaaatatta
     7561 aacaatgagg aacttaatct aatataattt ttggctagta tgactggaat aaaaataagc
     7621 tgaaaagagg aggtgattta gtgaaggtta atgggaagga aattgaattt gaaaaagatt
     7681 tgactgttat agatttatta aataaatata atttaaaaag tgatagagtt gtagttgaag
     7741 tgaatttaga gataattgaa gaatctaact acaatacata tgtattaaaa gacgaggaca
     7801 ttgtagagct tattagcttt attggaggtg gttaaaataa aaatatttgt aaatgaatta
     7861 ttaacagatg ttgaggaaaa tacaactgct tataaagtta gagatagcta taacaataaa
     7921 tgtgatgtat tggtgttaaa tggatatcca attaaaacag atatgaccct ttgtgaaaat
     7981 gataaattaa cacttataca aaaaggagtt aagccatcac ttgatgaatt ggaaagcttg
     8041 ttgattgcta gacatactcc aaatatacat aataaattaa aaaaagggaa agttgctata
     8101 ttgggattgg ggggacttgg ttccaatata gctatctctt tagctagaat aggagtggga
     8161 gaacttttac ttatagatta tgatgttgta gaaccatcta atttaaatag acagcagtat
     8221 tttatagatg atataggaaa actaaaaact gatgccatga tagaaaatat aaaaaaaata
     8281 aatccattta taaaattaaa taaacgagat atttttttaa acaagaacaa tatggatact
     8341 attaaggatt cagatttaat aattgaggcc tttgatgatg cttcatgtaa agcacaggta
     8401 tgtaattatg tattaataaa tttaaaagat aaatatttaa tagcatcatc tggtatggca
     8461 ggttactatg attcaaatat aataactact aagaagataa aagacaggtt ttatatatgt
     8521 ggggattttg tgaatgaagc taaatttggt gaagggctta tggctccaag ggttgccatc
     8581 tgcgcaaatc atatggcaaa tttggcaacg caaatactta tagatatgta aatattaaaa
     8641 ttagatataa aaaagattga atattaaaaa aatttaacgt ggagggaaat aatatggata
     8701 aattggtact tggtggacat gaatttaata gcagattatt ggttggaaca ggtaaatatg
     8761 gttcaaataa tatattgcct gaggtaataa aagaaagtgg aagtgaaata ataacaatgg
     8821 cacttagaag agtggattta gacaataaac aagaaaacat attaacgtat attccaaaag
     8881 agatgacaat acttccaaat acatcaggag ctactaatgc agaagaagct gtaagaatag
     8941 ctagaatatc tagaaaaatg ggatgtggag attttataaa aatagaagta atatcagata
     9001 ctagatattt acttcctgat aatgaagaaa ctataaaagc tacaaagata cttgctgatg
     9061 aggggtttat agttcttcca tatatgacac cagaccttta tgctggaaga agacttatag
     9121 aagctaatgc agcggcagtt atgccacttg gagctccaat tggttcaaat agaggtcttc
     9181 aaatgaaaga aatgataaga ataatgatag atgagttgga tataccaata atagtagatg
     9241 caggtattgg aaaaccatct caagctatgg aggctatgga aatgggagct gatgcagtac
     9301 ttgtcaacac tgctatagct tcagcaggag accctgtaca aatggctaga gcatttaaat
     9361 tagctgtaga aggtggaaga gaagcatata ttgcaaaaac aggaaatgtg tcagaatttg
     9421 caaatgcatc ttctccttta acaggttttt taggcaattt atagaaagtg gtgattggtg
     9481 atgagttttt atgatgtaat agaaaaatat agggattttg attttgatgg atatttaaat
     9541 aatgttactg ataatgatgt tttaagaagt ttatcaaaag acaagcttga agattttgat
     9601 atattaaatt tgctttctaa aactgcagta aaacatttag aagatatggc acaaaaagca
     9661 cataaattaa gtgttcaata ctttggtaaa acagtatgtt tatatacacc aatgtatata
     9721 gcaaattatt gtgttaatca atgtgtttat tgtagctata atataaagag tggaataaaa
     9781 agaaaaaaat taactatgga tgaaattaga gaagagggag aagtaatttc aaaagaaggt
     9841 ttcaaacatc tacttgtttt gactggagaa agtagttttc actcaagtgt agaatatata
     9901 ggtgaggcta tagaaatatt aagagagaaa tttccctcta taggtataga agtttatcca
     9961 atggaagtag aagaatataa gtacattgta gataaaggtg ttgaaggatt aacagtatat
    10021 caagaaactt atgacgagga aatatataag agagtacata taaaaggccc aaaatcaaat
    10081 tataaattta gactagatgc tcctgaaaga ggggcaaaag caggtatgag gactttatca
    10141 ataggtgcac ttttagggct aaatgatttt agaaaagaaa ctttttttac aatattgcat
    10201 gggaaatact taaaaacgaa atatcctcat attgagcttt catattcaac accaagaatg
    10261 agacctttta aagggtgttt tgaggaattg gtagatataa gtgatactga tttagttcaa
    10321 gctatggtat gtatgagatt atttgaccct catgcagcta taaatatatc cactagagaa
    10381 aatttagaaa tgagaagtca tataatacct ttaggagtaa ctaaattgag tgcaggtgta
    10441 tctactgatg taggaggtca ttctcaagat gaacatgata ctgctcaatt taagataaat
    10501 gatgaaagta ctgttaaaga tgtggagaaa atgctaaact caattggtta tcagcatgtg
    10561 tttaaagatt gggaaagatt ttaaatgtat ctgataacaa acagaaaatt atgtagtgag
    10621 gaacgatatt tagaagttat taaagagtct atattgagtg gtgtagaaaa tataataata
    10681 agagaaaagg atttagaata tcaagagcta agaaaattat atatgaaaat aaaaactaaa
    10741 atcaattgta tagattttca agagcaaata tcagatgaaa gtttgaaaac taatataaat
    10801 caaaaagagt ttagaaataa attcaaggtt aattttataa taaatagtaa cattgaattt
    10861 tttgaaaaag tggattgtca aggtatccat cttcctttta aattattctt gaatttaatt
    10921 gaaaataagt ataattttaa tgaaaataaa atactaggat tatctttaca taaagtagag
    10981 gaagtagatt atttagaaaa attaataaga aatcaaaata taaaaataga ttacataaca
    11041 ttatcacata tatatgaaac taaatgtaaa gaaggattaa atccaaaagg aattgagctt
    11101 ttaaaagaag ctaaaaagat tactgatatt aaaataattg cacttggggg aattttacca
    11161 tctaatgtca aggaaacatt aaaatactgt gatgattttg ctataatgtc aactataatg
    11221 aggtctaaag atattaaaaa aaccatatca aattataatg aaaagttgaa ttagtaaata
    11281 tagtatattg aatttttaac ttaaataaaa ataaaaaagt tatcttttaa attttaaaag
    11341 gtagcttttt tattttatat ctttgctaat aaaaaattgt aaaaagggaa aactttagtc
    11401 agagagatta aacaatatcc tagaaagatt taaaatttag taaagtatgg aggacattat
    11461 aatgactgac agtaaaaaga aacgatttat tataccacac acttttacaa taatattttt
    11521 actaatagtc ttgatggcaa tattgacttg gatagtacca agtggtgaat ttagtagaga
    11581 agatgtagaa ggtagaatgg tagttgtacc agggacttat caaagtgtag aaagtaatcc
    11641 tcaaggattt gcagatgttt ttaaagcacc aatagaaggc tttatcgatt cagctgaagt
    11701 tgtaggtttt gttttaatag ttggaggtgc ttttggtatt gtaaatagga ctggagctat
    11761 agaagcagga atagcttcgg ttatctctaa gtttaaggga aaagaacttc ttataattcc
    11821 aatatcaatg attttatttg gacttggagg tacaacattt gggatggcag aagagacatt
    11881 accattttat atgatatttg ttccactcat gacatccttg ggatatgatt ctctaacagc
    11941 aatagcaatt gtatttatag gagcagcagc aggagcagcc gcttcaacaa taaatccatt
    12001 ttcagttggt attgcacagg cactagctca acttgctcct ggttcaggag taacttacag
    12061 agtagttatt tttatagcat atatactaat aagtatagca tttgttatgt actatgctaa
    12121 gaaggtaaag gaaaatcctg aaagttcaat agtatatgaa atagataaaa ataataaatc
    12181 aatacttaat caaacttcaa cagaaataaa agaatttact tttaaagaag tatctgttct
    12241 tgctatattt ggaataggga tggcaataat gatttggggt gttttaagtc aaggatggta
    12301 cattccagaa atatcaatgt gttttgctgc aataggtgtg ctttctggat tggttggtag
    12361 attatctcaa attgaaatct cagaaagttt tatagatgga gcaaaagatt tagcatctgc
    12421 agctcttgct atagctttgg ctcgtggaat cgtaataatt gctcaaaatg gatttataat
    12481 agatactata ttaaattccg cagcagcctt tctaggtggg cttccaaagg ttatatttgt
    12541 atatttatca tttttcttgg aagctgcatt ggcattttta attccatcat cttcaggtct
    12601 tgcttcactt acagtacctg tacttgctcc tcttggtgat ttggttaata tatcggctca
    12661 agtgatagta accgcttatc aatttggtgt gggtcttaca aactttataa ctccaacttc
    12721 aggagtactg atgggagcac ttgcagtagc ccatattcca ttttcaaaat gggtaagatt
    12781 tataatgcct atcttgttga ttttgacagc tgtatctcta gtctttttaa caataggtat
    12841 ttatataggg ttttaattaa gtgtagtttt atttaagata tttagtaatg gtataaaatg
    12901 ataatatgtt ttcaataaat tgccactaat ttaattagtg gtaatttgtt ttttgtattt
    12961 aaaaataaat gtatttactt gtattttgaa ttaatatcta cttagacatt gttttaattg
    13021 ttatattttt atttaattaa gcattgattt atc
//
LOCUS       DAMJYR010000107        12867 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.110, whole genome shotgun sequence.
ACCESSION   DAMJYR010000107 DAMJYR010000000
VERSION     DAMJYR010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12867)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12867)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12867
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.110"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            131..319
                     /locus_tag="PC471_002886"
     CDS             131..319
                     /locus_tag="PC471_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HDF2789996.1"
                     /translation="MAKVCSVCGKGKVSGNQVSHSNKHNKRTWSANLRSVRAIIDGAP
                     KRVKVCTRCLRSGKIERA"
     gene            complement(479..631)
                     /locus_tag="PC471_002887"
     CDS             complement(479..631)
                     /locus_tag="PC471_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate translocator"
                     /protein_id="HDF2789997.1"
                     /translation="MRNNKSKVLLGILCLATGMAVILSMLLPGWIWSALTALILIGCG
                     ALLFFC"
     gene            complement(729..1205)
                     /locus_tag="PC471_002888"
     CDS             complement(729..1205)
                     /locus_tag="PC471_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HDF2789998.1"
                     /translation="MNLKRSNEKREEYRRMYSDKESFLSLIQNFTSVSIAKELTLKNI
                     ELETSFICEYKGKEVDIIYKVFSKSGKVSHYIVLEFQTEMDTEIVPRLKSYREQIWKS
                     FIMKKSLEEIEDKNFKLPKVIPVVLYSGPERLSRKKGILDIVESISEFSNNSSMKN"
     gene            1424..2185
                     /locus_tag="PC471_002889"
     CDS             1424..2185
                     /locus_tag="PC471_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDF2789999.1"
                     /translation="MEYTVQKLSKLAGISTRTLRYYDEIGLLKPLKINSSGYRIYGQN
                     EVNKLQQILFYRELGINLENIKNIINSPNFDSLSALKEHHNKLLAKRKQIDLLIENVT
                     KTIALKEGKYTMTDIEKFEGFKEKMIDENEKNYGTEIREKYGKDIIDTSNKKLKNMSK
                     QDYENWQNLNIEIISKLKKAFKTGDASSELAQEVAKLHHKWLCYTWNTYSKEAHSSLA
                     QMYVYDERFTSYYDREQPGLAKFLRDAIVIYTRDK"
     gene            complement(2445..4373)
                     /locus_tag="PC471_002890"
     CDS             complement(2445..4373)
                     /locus_tag="PC471_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HDF2790000.1"
                     /translation="MHKRLLTLFKLLNESDDKITCKTLSNHLKVSERTIRNDITSING
                     TLEKNGAIIKIKKGEGYYIDILNLALYQQYLALISDDIMDSSEIPDSPIERNQYILKY
                     ILYNNTYIKLEDLANSLYVSKFTILNDIKRIKPILSKYNLILVSKPYYGVKVEGKEID
                     IRRCISNNMINRNFENYIIGITDREIELFNNVDLIELQRVVLSEINKFNINFLDFNLK
                     NFIIHLAITVSRILDGYCLDNVLDVVLTDFQSNTTVENIFNYIESKYTIIISKADRVY
                     LYNHFITKSSLLDNVSNRVDTKIIEYVEEILEVINNQYTFDLRNDSVLFDDLVLHFKS
                     ILNSKSYNLNKVNPLINTIKSNYPLAFEITLNAIEKVFKNSIYSLTEDEIGYVSLHIG
                     AGIERFFQSNIKCKNVVLVCGSGYGSSRLLEVQLNKVFHDKINILQCLSFNQFLASEL
                     SDVDIIISTIPLNHDSIPIVLVDLKLLKKDIENISKSITNNSHIYSNLLDNFFDKNLF
                     IVNPKIKDKDELIKLMCNKLTQSEIVFPSFAESVFYRESLSSTNIDDFLAIPHPMELS
                     SIRTKICISILNEPIYWSENSTVKLIMMLAINKDDYIKINSIYDILLKIIHDNDIRDS
                     ISNCNDFDNFLSIIKSIV"
     gene            4607..5059
                     /locus_tag="PC471_002891"
     CDS             4607..5059
                     /locus_tag="PC471_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDF2790001.1"
                     /translation="MVLQEKYIFIDKDLKSKEEVLNFIADSAYILGITTNKEALLKDL
                     LKRENEFPTGIKDGFAIPHTKSVNVKEASILYIKTTNELEWKTLDDSKVRYVFSLLAP
                     HKNEGNIHLKTLSKLATCLMEDDFTSEVKNASDASHLVKYITNKMKEE"
     gene            5062..5370
                     /locus_tag="PC471_002892"
     CDS             5062..5370
                     /locus_tag="PC471_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HDF2790002.1"
                     /translation="MKIVAITSCIAGLAHTPMAAKALEQAASKMGHEIKVEQQGAMGR
                     VNTITKEEAQDADFILIASDQTISGMERFDGKKVIKVKIGIALKKPEAVLTKCIEAIS
                     "
     gene            5484..6590
                     /locus_tag="PC471_002893"
     CDS             5484..6590
                     /locus_tag="PC471_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HDF2790003.1"
                     /translation="MSKKNKSLLSELKGHLMTGISYMLPLVIGSSLVVAIPKLTALAV
                     GITSLDAYKNVSGFYHILYLMEQVGWTGIGLLNTVLAGFIAFSIGEKPAMGAGFIGGL
                     IASNTNAGFLGAVIAGFFAGYVCKFLKKKIKISGSASGMMPLIIMPLITVGLTGFLMS
                     VVLAGPLGNINTSLNAWVAEMCQNGTNSVVLALILGAMIGFDLGGPVNKAAWMAGNAL
                     LLEGIYLPAILVNCAIVIPPLGYGLATFIRKSKFSNALAETGKGNIVMGIIGITEGAI
                     PFTLTNPLKLVPVNMIGCALGVGLSALLGVHAIMPPVGGLYGFISIGSGWAYLVGAIF
                     GALVIAILSTTLVDFNEDETTENTEFDEIELDIM"
     gene            6665..9343
                     /gene="mngB"
                     /locus_tag="PC471_002894"
     CDS             6665..9343
                     /gene="mngB"
                     /locus_tag="PC471_002894"
                     /EC_number="3.2.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosylglycerate hydrolase"
                     /protein_id="HDF2790004.1"
                     /translation="MKKTKVHVVPHSHWDREWYFTTSRSKIYLMKDFQDILDILEEKE
                     DFKYFTMDAQASLLDDYLKWRPQDKYRIRDLVQRRRLVIGPWYTQTDQLVISGESIIR
                     NLYYGIDRCNEFGEPMRVGYVPDSFGQSAQMPQIYRGFGIDSSLFWRGVSDDMVDTTE
                     FIWKGSDGSKVLAVQIPFGYYYGGNIPEDDSDLKEYLKDIIVKLKVKASTNNVYFPNG
                     FDQAPIRKNLPDILKKANEIDNENEYEISDIENYIDSVRNERNEFTELKGEFLNAKHM
                     RIHKSIFSSRSDLKIMNNKLENYITNVLEPLLTLSYSLGNEYPHLVIKDIWKLMFENA
                     AHDSIGSCNSDTTNEDVYTRYKQARDLSINLLDLHMRLISTQIKNNNEEITLTVFNGF
                     PEVRNEVVEFDTYIPGQDFVIKNKNGKILNYIIKEKEDITNYILTQTIRLNPSKKIYI
                     PKTVYRAKIALSVKDIPPMGYTQLVFELDTKNKCEIETSNCKEIENKYYKITANKNGS
                     LEILDKESNKTYKNQAVIEDNGDDGDSYNYSPPRKDIYVSSLDSVSSVEVLKSNIQEE
                     MILKYSLTIPASLEERAIGKVSVKMPVTMKITLEPKEQIIKFNVNIKNNQALSHRVRV
                     LFNTEIASKFSIADQQFGIIQRPTVLEKDLALWKRDNYSWQEKPITIEPMQSFVTLED
                     GQRGIALITGCVREYQIVGDNLDTIALTLFRSFGYMGRENLLYRPGRASGEKIIATPD
                     AQLLKELNFDFGLYIYNNKFDEANVANTAKRFLTPIQIYEYADFLNGRLIFAFNDEKQ
                     IYENEFSLMNVNNSNFTVSAIKKEEKGDGIVVRIFNGMKNEDAKGSLNINKNVNDAYS
                     AMLDEIYDNTSKLKVNTKTVEVNSLSHCKVQTIVIK"
     gene            complement(9660..11993)
                     /locus_tag="PC471_002895"
     CDS             complement(9660..11993)
                     /locus_tag="PC471_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HDF2790005.1"
                     /translation="MDTKLKKFKDKYIVTIIVFILMLSASLGMISQYSTIQASAKGGA
                     ENPFEQESFVDSIYKGSYVLYHNMKEEQEGKMIQPSKLFINEETMNYIKNVSKEKDTY
                     DGSETYYTDDSINADFNENFENWEHFIKASGKNLKYYLVDKESNFEFFNDNKELGVLD
                     TTKNDASKENSDENQSKNTEMNDEERQKQITDIKENYRFYVVMNFDKDGKVNIVDSYG
                     VNQKIINQMLLSKAREDLMDTSLGGNSISYKLSPIKNITLVYAVPKVISKGDSVEYGY
                     GNTYFDDGIISYISNVENNSYYKISGMILKIILAIVVIVALAFPYRKSKELLGFDTLS
                     RIPLEILFIALAIIQGLVDLYPTVIISNTLSGNYVEYLINNDISAKMASILVSLMNLT
                     YWFVIFSIIFVFIILLKHILNVGIREYFTKHTVTGMFFVWLKKAGKTVINQIRTINLK
                     EKPNKTIVTILGINLLIIVIMCSMWFFGIILALIYSVVLFKILSDYSKKTIYEYNQLL
                     DVTKKISDGNLDVNMEKDLGFFNPIKDELGNIQTGFKKAVDEEVKSQKMKTELISNVS
                     HDLKTPLTSIITYIDLLKDESITDENRKLYIDTLDRKSQRLQHLIEDLFEVSKANSGD
                     VYLNIVNVDIVSLMKQTLLELDDKLADSSLIVKNNFSNEKIILPLDSQRTFRVFENLI
                     INISKYAMPNSRVYIDILETDNKVNIMLKNMSASEIDFSVDDIMERFVRGDKSRNTEG
                     SGLGLAIAKSFIELQGGKMNISVDGDLFKVTITFNKN"
     gene            complement(11968..12684)
                     /locus_tag="PC471_002896"
     CDS             complement(11968..12684)
                     /locus_tag="PC471_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2790006.1"
                     /translation="MNSYNILVVEDEKEIADAIEIYLLNQGYNVFKGYNGLEGLKVIE
                     NQEIHLAIVDIMMPKMDGITLTMKLRENYNFPVIMLSAKSEEVDKIMGLNIGADDYVT
                     KPFKPLELLARVNSQLRRYTKYLNMVGNKEQKVESDDSVFAIGGLELNENTKEVSIDG
                     KYIKTTPIEFKILSLLMRNAGRVFSADEIYERVWNENAVNTDTVMVHVRNIREKIEID
                     PKNPKYLKVVWGVGYKIEKI"
BASE COUNT         4792 a         1793 c         1673 g         4609 t
ORIGIN      
        1 tatgataata agaaattctt caatatgtgt aaggcaaata tatatttcta ttgcaaattt
       61 ttgttattta tgataatata aatatgtttt tagtctttaa atagttatca ttttaaggag
      121 gtgtgcgtag atggcaaaag tatgtagcgt atgtggtaaa ggtaaggttt ctggaaacca
      181 agtatcacac tcaaataaac ataataaaag aacatggtca gctaacttaa gaagcgtaag
      241 agcgattata gatggagctc ctaagagagt aaaagtttgt actagatgtt tacgttctgg
      301 taaaattgaa agagcttagt aaccacgttt aaaagcctac ttccgtaagt aggcttttta
      361 atttaatcat ttttactctt tctcataaat tttaggatta tacttgaaat ccattttggt
      421 aattttattg ttactacttt catcaatact caccccacac tttatagtta aaactatttt
      481 aacaaaaaaa taataatgct ccacatccaa taagtattaa tgcggtaagt gctgaccaaa
      541 tccagccagg aagcaacatt gacagaatta cagccattcc tgttgctaga caaagtattc
      601 ctagcaatac cttagacttg ttatttctca tggaacacct ccacatagaa cctgtatcta
      661 taaatatttt catattcaag tttatgcgac agttttccat tttagaccaa taaacacatt
      721 atgtaatttt aatttttcat actagaatta ttactgaact ccgatataga ttctacaata
      781 tccaaaattc ctttttttct acttaacctt tcaggtccac tatataaaac aactggtata
      841 actttaggaa gtttaaaatt tttatcttct atttcttcaa ggcttttttt cataataaaa
      901 cttttccaaa tttgttctct atacgatttc aatcttggga caatttcagt atccatttct
      961 gtttgaaatt ctaacactat ataatgtgat actttgccgc ttttagaaaa taccttataa
     1021 attatatcaa cttcttttcc tttatattca catataaaag atgtttctaa ctctatattt
     1081 ttaagagtta attcttttgc tattgagaca cttgtaaaat tttgaatcaa acttaaaaaa
     1141 ctttctttat ctgaatacat tcttctgtac tcttctcgtt tttcattgct tctttttaaa
     1201 ttcatacagt tcctcctaga ctatagacct tctatttttt agtaaagata gtctataaat
     1261 tttatttttt aattttcact agtgagtatt gacagttgtg tgtaaaatat ccagtaaaga
     1321 tattttttta tatttttatt aaaagatata aatttttata ttttaatgta ttgacattta
     1381 cgttgcgtaa aagtgtatat ttaatttata ggaggtacta cttatggaat atacagttca
     1441 aaaattaagt aaactagctg gtataagtac tagaacactt agatattatg atgaaatagg
     1501 tcttcttaag ccacttaaaa taaattcttc aggttatcgt atatatggtc aaaatgaagt
     1561 taataaactt caacaaatac ttttttatag agaattagga attaacttag aaaatataaa
     1621 aaacattata aattcaccaa acttcgatag tttaagtgcg ttaaaagaac accataataa
     1681 attactagcc aaaagaaagc aaattgattt actaatagaa aatgtaacta aaactatagc
     1741 tctgaaggag ggaaaataca ctatgacaga tatagaaaaa tttgaaggtt ttaaagaaaa
     1801 gatgattgat gaaaatgaaa aaaattatgg aactgaaatt agagagaaat atggtaaaga
     1861 tattatagat acatcaaata aaaaacttaa aaatatgtct aagcaagatt atgaaaattg
     1921 gcaaaaccta aatatcgaaa taattagtaa acttaaaaaa gcttttaaaa ctggtgatgc
     1981 ctctagtgaa ctggctcagg aagttgcaaa attacatcat aaatggttgt gttacacttg
     2041 gaacacatac tctaaagaag ctcattcctc tctagcacag atgtatgtat atgatgaacg
     2101 ttttacatca tattatgata gagaacaacc tggattagct aagtttttaa gggatgctat
     2161 agtaatatat acaagagata aataatataa aataataaag gtggtgtctc aaaatgaact
     2221 ttttagttca tgagacactc ttttttgtat gaaatcagat atatttttat cattttatca
     2281 atgaaaaaca ggtttctctc tctattttga gacagaatct ttattgtata ataattataa
     2341 tcaatacaaa ataataacaa attatacaaa acttacttat actatttatg ctaaaaacaa
     2401 aaaatatttt attaaagttt ttaaaactta tgtaatataa aaatctatac tatagacttg
     2461 attatggaaa gaaaattatc aaaatcatta caattagata tactatctct aatatcattg
     2521 tcatgaatta tctttaaaag gatatcataa atggagttta tttttatata atcatcttta
     2581 tttatagcca acatcatgat taatttaaca gtagaatttt cactccaata tattggttca
     2641 ttcaatatgg aaatacaaat cttagttctt atagagctta attccatagg atgaggtata
     2701 gctaaaaaat catctatatt ggtagaagat aaactttctc tgtaaaatac agattcagca
     2761 aatgatggaa atacaatttc actttgagtt aatttgttgc acatcaactt tataagctca
     2821 tctttatctt tgattttagg attaacaata aataaatttt tatcaaagaa attgtccaaa
     2881 agattactat aaatatggga attattagtg attgacttag atatattctc tatatctttt
     2941 tttaatagtt ttaaatccac taatacaata ggaatactat catgatttaa aggaattgtc
     3001 gatattatta tatctacatc tgacaattca ctagctaaaa attgattaaa tgatagacat
     3061 tgaagtatat ttattttatc atgaaaaact ttgtttaatt gaacctctaa taacctactt
     3121 gaaccatacc ctgaaccaca tactaatact acgtttttac acttaatatt actttgaaaa
     3181 aatctctcta ttccagcacc tatatgcagg gatacatatc caatttcatc ttctgttaaa
     3241 gagtaaatac tatttttaaa aactttttct atagcattta aagttatttc aaatgctaaa
     3301 ggataattac tctttattgt atttataagg ggattcacct tatttaaatt ataagattta
     3361 gaattgagaa ttgatttaaa gtgcagtact aaatcatcaa ataatacact atcatttcta
     3421 aggtcaaaag tatattgatt gttgattact tctaaaattt cttcaacata ctctataatt
     3481 ttagtatcta ccctattgga aacgttatcc aaaagagaac ttttagttat aaaatgatta
     3541 tatagataaa ctctgtctgc ctttgaaata attatagtat atttactctc aatataatta
     3601 aatatatttt caacagttgt attcgattga aaatctgtta aaacaacatc taaaacatta
     3661 tctaagcagt aaccatcaag tattctactt acagtaatag ctaaatggat aataaagttt
     3721 tttagattaa aatctaggaa attgatatta aatttattaa tttcacttaa cactaccctt
     3781 tgaagctcta ttaaatctac attattaaat aattctattt ctctatctgt aattccaatt
     3841 atatagtttt caaaattcct atttatcata ttattagaaa tacatcttcg aatatctatt
     3901 tccttacctt caactttaac tccataatat ggcttagaaa ctaatattaa attatacttt
     3961 gacaaaatag gctttattct ttttatgtca tttagtattg taaatttact cacatacaag
     4021 ctattagcta aatcttctag ttttatatat gtattgttat agagaatata ttttaaaata
     4081 tattgatttc tttctatagg agaatctggt atttcacttg aatccataat atcatcagat
     4141 attaatgcta gatactgttg gtataatgct aaattaagaa tatctatata atatccttct
     4201 ccttttttta ttttaataat agcaccattt ttctctaaag ttccatttat agaagtaata
     4261 tcatttctta tagttctctc actcactttt aaatgattac ttaaagtttt acaagtaatt
     4321 ttatcatcac tttcatttaa taatttaaac aacgtgagca atctcttgtg cataaatatc
     4381 cccccttata gaatttcttc taaattatac tatttacact catataatac aattactcta
     4441 ataaaattgt attataataa tttatttcca cttctatatg gaaattagtt attatcataa
     4501 tataaatttc catgtcgaag tggaaattag ttatatttaa tttaattagg ataatgatta
     4561 caatataaat ataagttaag acattatgaa tggagtggaa aaatatatgg tactacaaga
     4621 aaaatatata tttatagata aagacttaaa aagcaaagaa gaagtattaa attttattgc
     4681 agatagtgct tatatactag gtattacaac taataaagag gctcttttaa aagaccttct
     4741 aaaaagagaa aatgaatttc caacaggaat aaaagatgga tttgcgatac ctcatacaaa
     4801 gagtgtaaat gtaaaggaag cttccatact ctatattaaa acaactaacg agttagaatg
     4861 gaaaacttta gatgatagta aggttagata tgtatttagt ttgttagccc cacataaaaa
     4921 tgaaggaaat atacatttga aaactctatc taaattagcc acatgtttaa tggaggatga
     4981 ttttacaagt gaagttaaaa atgctagtga tgcaagtcat ttagtaaaat atataactaa
     5041 taaaatgaag gaggaataat tatgaaaata gtagctataa catcatgtat agcaggatta
     5101 gcacacacac ctatggcagc aaaagcttta gaacaagctg catcaaaaat gggtcatgaa
     5161 atcaaagtag aacaacaagg tgctatggga cgagtaaata ctataacaaa agaagaagca
     5221 caagatgcgg actttatact tatagcatct gaccaaacta tatctggaat ggaaagattt
     5281 gatggtaaaa aagtgataaa agtcaaaata ggtatagcgt taaaaaaacc agaagctgtc
     5341 ttaacaaagt gtatagaagc tatttcttaa taaaaaaatc tcactctaat atagaatgag
     5401 aaaaatcaaa aattaaaaaa ttgtctaatg taattataac atattatcaa aaaatataac
     5461 aaacttatat aggaggatta aaaatgagta aaaaaaataa atctctttta agtgaactta
     5521 agggacactt aatgacaggt atctcatata tgttgccact tgtaataggc tcatctttag
     5581 ttgtagctat cccaaagctt acagctttag cagtgggtat aacaagttta gatgcttata
     5641 aaaatgtatc tggtttttat catattcttt acttaatgga acaagttggt tggactggca
     5701 taggtctatt aaatacggta ttagcaggat ttatagcatt ttcaataggt gagaaaccag
     5761 ctatgggtgc aggttttatt ggtgggctca tagcaagtaa tactaatgca ggattcttag
     5821 gagcagtaat agcaggtttc tttgctgggt atgtttgtaa gtttttaaag aaaaaaatta
     5881 aaatatctgg gtcagcatca ggaatgatgc cacttataat aatgccactt ataacagttg
     5941 gattaactgg ttttttaatg agtgttgtat tagcaggacc tttaggaaac ataaatactt
     6001 cattaaatgc ttgggtagct gaaatgtgtc aaaatggaac aaattcggtg gtattggctt
     6061 taattttagg cgcaatgata ggattcgatt taggtggacc agtaaataaa gcagcttgga
     6121 tggcaggaaa tgcattattg ttagagggaa tctatctacc agcgatattg gtaaactgcg
     6181 caatagttat acctccacta ggatatggtt tagctacatt cataagaaaa tcaaaatttt
     6241 ccaatgcatt agctgaaaca ggaaaaggca atatagttat gggtataatt ggaataacag
     6301 aaggtgcaat tcctttcact ttaacaaatc cattaaaatt agttcctgtt aatatgatag
     6361 gatgcgcttt gggtgtagga ttatcagctt tgttaggtgt acatgctata atgcctccag
     6421 ttggtggatt atatggattt atatcaatag gttctggatg ggcttactta gtaggtgcaa
     6481 tatttggagc tttagtaata gcaatattat caacaacttt agtagacttc aatgaagatg
     6541 aaactacgga aaatacagaa tttgatgaaa tagagttaga tataatgtaa attaaaaact
     6601 taattttaga taattgagaa ttgagtgtac tctcaattct caattaaatt ttgggaggat
     6661 ttttatgaaa aagacaaaag tacatgtagt tccacacagt cattgggata gagaatggta
     6721 ttttacaaca tcaaggtcaa aaatatattt aatgaaagat tttcaagata tattagacat
     6781 attggaagaa aaagaagact ttaaatactt tactatggat gcacaagctt ctctcttaga
     6841 tgattattta aaatggagac ctcaggataa atatagaata agagatttag ttcaaagaag
     6901 aagattagtt attggacctt ggtatactca aacagaccaa ttggttatat caggagaatc
     6961 tataattaga aacctttact atggaataga tagatgcaat gaatttggtg aacctatgag
     7021 ggttggatat gtaccagatt cttttgggca atcagctcaa atgccacaaa tatacagagg
     7081 ttttggaata gatagttcat tattttggcg tggagtttct gatgatatgg tagatacaac
     7141 agagtttata tggaaaggtt ctgatggttc aaaagtatta gcagttcaaa taccatttgg
     7201 ttattactat ggaggtaata taccagaaga tgattcagat ttaaaagaat atttaaaaga
     7261 tataatcgta aaacttaagg ttaaagcttc tactaataac gtatattttc caaatggatt
     7321 tgaccaagca ccaataagaa aaaaccttcc tgatatatta aaaaaagcta atgaaataga
     7381 taatgaaaat gaatacgaaa taagtgatat agaaaactat atagattcag taagaaatga
     7441 aagaaatgaa tttacagagc ttaaaggtga atttttaaat gctaaacata tgagaataca
     7501 caaatccata ttctcctcaa ggtcagacct taaaataatg aataacaaat tagaaaatta
     7561 tattacaaat gtacttgaac cgttattaac attaagttat tcacttggaa atgaataccc
     7621 tcacctagtt ataaaagata tatggaaact tatgtttgaa aatgcagctc atgacagcat
     7681 aggttcttgt aatagtgaca caacaaatga ggatgtatac acaagataca aacaagccag
     7741 agatttgagt ataaatttat tagatttgca tatgagactt ataagtactc aaatcaaaaa
     7801 taataatgaa gaaataacat taacagtatt caatggattc ccagaagtca gaaatgaagt
     7861 tgtagaattt gatacttata ttccaggtca agattttgtg ataaagaata aaaatggaaa
     7921 aattcttaat tatataataa aagaaaaaga agatattaca aattatatat taactcaaac
     7981 aataagatta aacccaagta aaaaaatata tatacccaaa actgtatata gagcaaaaat
     8041 tgcactttca gtaaaagata taccaccaat gggatatact caactagttt ttgaattgga
     8101 tactaaaaat aaatgtgaaa tagaaacaag taattgcaag gaaatagaaa acaaatacta
     8161 caaaataaca gccaataaaa atggttcatt agaaatatta gataaagaat ctaataagac
     8221 ttataaaaac caagctgtaa ttgaagacaa tggagatgat ggagattcat acaactattc
     8281 tcctccaaga aaagatattt atgtttcatc tttagattct gtatcttcag tagaagtgct
     8341 aaaatcaaac attcaagaag aaatgatact taaatattca ttgaccatac cagcttcact
     8401 ggaagaaagg gctataggta aagtttctgt gaaaatgcca gtaacaatga aaatcacctt
     8461 agaacctaaa gaacaaataa ttaagtttaa tgttaatata aaaaataacc aagcactatc
     8521 tcatagagta agagttttat ttaatacaga aatagcatct aagttttcta tagcagatca
     8581 acaatttgga ataatacaaa gaccaacagt attagaaaaa gaccttgcat tgtggaaaag
     8641 agacaattac tcatggcaag aaaaacctat aactatcgaa ccaatgcaaa gttttgttac
     8701 tttagaggat ggacaaagag gaatagcatt aataactggt tgtgtaagag aatatcaaat
     8761 agttggtgat aatttagata caatagcatt aacactattc aggtcatttg gatatatggg
     8821 aagagaaaat ttactttata gaccaggaag agcttctgga gaaaaaataa tagcaacacc
     8881 agatgcacaa ttacttaaag agttaaactt tgactttggt ctttacatat ataacaataa
     8941 atttgatgaa gcaaatgtag ctaatacagc taagagattc ttaacaccta tacaaatata
     9001 tgaatatgct gattttttaa atggtagact tatatttgca ttcaatgacg aaaaacaaat
     9061 atatgaaaat gaatttagtt tgatgaatgt aaataattca aatttcacag taagtgcaat
     9121 aaagaaagaa gaaaaaggag atggaattgt agttagaatc ttcaatggta tgaaaaatga
     9181 ggatgctaag ggaagtctta acatcaataa aaatgtcaat gatgcttata gtgcaatgct
     9241 cgacgaaatc tatgataata ctagcaagct taaagtaaat actaaaactg tagaggtaaa
     9301 ttctttatca cattgtaaag tacaaacaat agtaattaaa taatataaaa tagcttatac
     9361 aagatttcaa aataaattgc tagtaccatg ttaatagtaa agccagatat tatgctattt
     9421 ataagctctg taacactaaa taacattttg taaaataaaa aggtgaacct tgtatattag
     9481 gattcacctt ttttaaataa atcaacattc tgttttaaaa taacatttat aagctgtttt
     9541 gaaataatgc ttacaaatta ttttaaaaca atacctataa atttttattt aaagaataca
     9601 cataaatatt tccagactct tttatcctat agactttata attattaaag aagtctaatc
     9661 taatttttgt taaaagtaat tgtaacttta aataaatcac catccacact tatattcatc
     9721 ttaccaccct gtaattctat aaaacttttt gctatcgcta aaccaagacc agaaccttct
     9781 gtgttacgtg atttatctcc tcttacaaat ctctccatta tatcatctac actaaaatca
     9841 atctcagatg cagacatatt tttaagcata atatttactt tattatctgt ttctaaaata
     9901 tcaatataca ctcttgaatt tggcattgca tatttgctta tatttattat aagattttca
     9961 aatactctaa atgtacgctg actatctaat ggtaatatta ttttttcatt agaaaagtta
    10021 ttctttacta ttagagatga atctgcaagt ttgtcatcta attctaaaag tgtctgcttc
    10081 attagagata caatatccac atttactata ttcaaataaa catctccact atttgccttg
    10141 cttacttcaa acaaatcttc tattagatgt tgcaatcttt gtgactttct gtctaatgta
    10201 tcaatataca acttacgatt ttcatctgtt atactttcat cttttaataa atctatataa
    10261 gtgattatag atgttagagg tgtttttaag tcatgtgata catttgatat aagctctgtt
    10321 ttcatctttt gactcttaac ctcttcgtca acagcttttt taaatccagt ttgaatattt
    10381 cctaattcgt cttttatagg attaaaaaat cctaaatctt tttccatatt tacatctaag
    10441 tttccatctg aaattttctt tgtaacatct aaaagttgat tatattcata tattgtcttt
    10501 ttagagtaat ctgacagtat tttaaataat actactgaat atattaatgc gagtattatt
    10561 ccaaagaacc acatactaca cattataact ataatcaata aatttattcc caaaattgta
    10621 actatagttt tatttggctt ttcttttaga tttattgttc taatttgatt aataactgtt
    10681 tttcctgctt tttttagcca tacaaaaaac attcctgtca cagtatgttt tgtaaaatac
    10741 tctcttattc caacatttaa aatatgtttt aaaagtatta taaatacaaa tattattgag
    10801 aatattacaa accaataagt taaattcatt aaacttacca atatactagc cattttagca
    10861 cttatgtcat tgtttataag gtactctaca taattcccac tcaaggtatt acttattata
    10921 acagtaggat ataaatctac aagcccctgt ataatagcaa gagcaataaa gagtatctct
    10981 aacggtattc ttgataatgt atcaaatcct agcaactctt tagattttct atatgggaat
    11041 gctaaagcta caataacaac tatagctaat ataattttta aaatcattcc agaaatctta
    11101 taataagaat tattttctac attgcttata tatgaaatta taccatcatc aaaataagta
    11161 tttccataac catactctac actatcacct ttacttatta ctttaggtac agcatatact
    11221 aatgttatat tttttattgg agataactta tatgaaattg aatttcctcc taaacttgta
    11281 tccattaaat cttctcttgc tttagataac aacatttgat taataatttt ttgatttact
    11341 ccataagaat ccacaatatt tacttttcca tctttatcaa aattcattac aacgtagaat
    11401 ctatagtttt cttttatatc tgtaatctgc ttttgtcttt cctcatcatt catttctgta
    11461 ttttttgact gattttcatc agaattttct tttgaagcat cattttttgt tgtatcaagt
    11521 actcctaatt ctttattatc attaaaaaat tcaaaattgc tttctttatc aactaaatag
    11581 tactttaaat tctttcctga cgctttaatg aaatgctccc aattttcaaa gttttcatta
    11641 aaatctgcat taatactatc atctgtatag taagtttctg aaccatcata agtatctttt
    11701 tctttactta cattttttat atagttcata gtttcttcat tgataaatag ttttgatggt
    11761 tgtatcatct taccttcttg ttcttccttc atattatgat ataaaacata acttccttta
    11821 tatatactat ctacaaagct ttcctgttca aatggattct ctgctccacc tttagcactt
    11881 gcttgtatag tagaatattg actaatcata cctaatgatg ctgataacat aagtataaaa
    11941 actataatag taactatata tttatcttta aattttttca attttgtatc caacacccca
    12001 caccaccttt aaatatttag gattttttgg gtcaatttct atcttttccc ttatattcct
    12061 cacatgtacc ataactgtat ctgtatttac agcattctca ttccaaactc tttcatatat
    12121 ttcatcagca gaaaaaactc ttcctgcatt tctcataagt aaacttagta ttttaaattc
    12181 tataggagtt gtttttatat actttccatc aatactaact tcttttgtat tttcatttaa
    12241 ttctaatccg cctattgcaa acacactatc gtcactttct accttttgct ctttatttcc
    12301 aaccatgttt agatacttag tgtaccttct aagttgtgaa ttaactctag ccaatagttc
    12361 taatggctta aaaggttttg ttacataatc gtctgctcct atattaagcc ccattatttt
    12421 gtcaacttcc tctgattttg cagatagcat tattactgga aaattatagt tttctctaag
    12481 cttcattgta agtgttatac catccatttt aggcatcatt atatcaacta tagctaaatg
    12541 tatttcttgg ttttctataa ccttaagacc ttctaatcca ttgtatccct taaaaacatt
    12601 atatccttga tttaatagat atatttctat agcatctgct atctcctttt catcttctac
    12661 caccaaaata ttatatgaat tcattctaat ctcctttata ttaatttata aattttcttt
    12721 acatatatga ttatacaata tgatagaagt ttttccattt atatatatat ttatatcttt
    12781 tgttctggtt atttgtattt aatgtccatc ttagaaacct ccttctagtt taaatttaat
    12841 attttttcat tttgttttat atactta
//
LOCUS       DAMJYR010000108        12855 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.111, whole genome shotgun sequence.
ACCESSION   DAMJYR010000108 DAMJYR010000000
VERSION     DAMJYR010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12855)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12855)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12855
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.111"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     regulatory      41..143
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            237..1613
                     /locus_tag="PC471_002897"
     CDS             237..1613
                     /locus_tag="PC471_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HDF2790007.1"
                     /translation="MQTTNSSTMQKLFPILTNKNVDMKKEIIAGVTTFLTMAYIIAVN
                     PNILSETGMPAGALVTGTCLAAAFGCILMGVVANLPFALASGMGLNAFFAYTVVLQMG
                     VPWEVALTAVFVEGIIFIVLSVSGVREAVVNAIPKNMKLAVTGGIGIFIALIGLVNSG
                     IVIGDQATLIKMGRFTPAVIITCVGLIIIAVLDKKRVKGSILFGIVVSSLLAWAFAFI
                     NPEYAQKLGIYLPGGLFKFESLAPIAGKIDLGYVLHPTNIGGFLVVVCTFLFVDFFDT
                     VGTLVGVCSKANMLDEKGNVPNVGRALLTDAVATTVGAGLGVSTVTTYVESSTGVIAG
                     GRTGWTAITVGILFLAAMFFSPVFIAIPSCATAPALIYVGYLMLGTVKDIEFDNITEG
                     VPAFVTIACMALTYSIGDGLTLGILTYVFVNIFYNIFGAKKAEDKKHVSVVMIVLAIL
                     FIIKLWLL"
     gene            complement(1727..2872)
                     /locus_tag="PC471_002898"
     CDS             complement(1727..2872)
                     /locus_tag="PC471_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HDF2790008.1"
                     /translation="MNTFEQLKISSTLIDGLKKQDITSPTEVQSLVIGNIIQNKDLLI
                     NSQTGTGKTLAYLLPIFEKIDTSKRETQALILAPTHELVMQITNQVELLAKNAELSVT
                     SLALIGEVNIQKQIKNIKAVKPHIVIGSCGRVLDLIKQKKLKSHNIKTIILDEVDNLL
                     NGKNITCIEDIIRTTLRDRQIIGCSASLTDSTIKICDKLMKEFEIIKTKEKSQINPNI
                     NHSYLLGEIRDKFTFLRKALAATNPKKAIVFVNNEKNIEVLVSKLNYHNYKAIGIFGN
                     MEKEDRKNAINKFKLGKAKILITTDLSARGLDIVDVSHVFNLDFPKSKNEYLHRCGRT
                     ARGNRSGNTISIITKKELDIIKDLQKEFNIVITPKTLQNGELIDIIK"
     gene            3125..3832
                     /locus_tag="PC471_002899"
     CDS             3125..3832
                     /locus_tag="PC471_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2790009.1"
                     /translation="MLKVINVSKSYKNKKVVSSVSFDVNDGELFGFIGHNGAGKTTTI
                     KAIVGIHDFEEGEVLINSKSIKKQPVECKKEMAYIPDNPDLYESLTGIQYLNFIADIF
                     EVAKDKREELIKYYSNKFELNKALGDLISSYSHGMKQKLAIISALIHSPKILILDEPF
                     VGLDPKASFILKEIMREFCDKGGCIFFSTHVLEVAEKICDKIAIIKDGNIIAYGTTEE
                     VKGSNSLESIFMELIEK"
     gene            3879..5474
                     /locus_tag="PC471_002900"
     CDS             3879..5474
                     /locus_tag="PC471_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2790010.1"
                     /translation="MSNLSILIKTNIINEFKLNALKKADGREKQKIMGMMLSVVIIAG
                     LLIFYVTSLSLSLVDILKQINQLEMLLIFGFGLSTLTTIVTSLYKTSSYLFQAKDLDL
                     LTSLPIKESTVLASKILMLVGANYLFSSICMIIPAVVYFLNSDVSITYFPYLILLFLA
                     LPLLPIVASSIIFLFIGQISSRIKYKNLVLIIGSIILMLLYTLLMSRLNSMSVEMLKN
                     SVSISETIRKVYPPIYYFVDALKTGSIVSFLAFIAISIIPFFMFVTLFAKKFKDINAK
                     MSEGYKSKNYEFKGQKTSRPSKALLKKEFKRYTSSFIYFLNSSAGLLFLMVGTILILL
                     FGADKVGELLKLNIDFNLIKIQLLGVFFFIIVMNCTTYCSISLEGKNLWILKSSPIDE
                     REIFWSKILMNFLLNAPLSILCLILVSIRLDFEIQFIIVSICLIVSFSMFVALMGLLS
                     NLLYPNLDWKNEVAVVKRSASMIVTMLVSLAYVIILGGIYVLLGISFLNIYIILASIL
                     TLILDFMIWRIISTKGVRIFKNL"
     gene            5577..6659
                     /locus_tag="PC471_002901"
     CDS             5577..6659
                     /locus_tag="PC471_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HDF2790011.1"
                     /translation="MRKLSKREKDFIQYGFLLFLICITTYLVLTTLDIKLIPKIIRLV
                     NKTYIFLGILTIIVYMIIESIVTHLIINAVQKTKVKFIGIKMATMGFYYNLVTPFASG
                     SQPMQIYALTKYDVSLSKSVAIVTNKTVVFQSTVTVFCGILILLNKSLLMEQVHSVKV
                     LITFGMIMNVSMLLGGLLIVFSPKQVKMIAEVLINFLSKFKTFKFLDSKRDRINHYID
                     DYNYSIKIFIKNVRILVLSILLTVLQLIAYFSIVYCVYKASNLRGVSYVHIMTLQVFL
                     YMAISPIPTPGNVGANEVTFFTMFSNIIPKELLGYSVLLYSGFVYYFILIVSGLFTFG
                     THYTLNSWKKENKVNSNLDKVIEKLN"
     gene            6808..6969
                     /locus_tag="PC471_002902"
     CDS             6808..6969
                     /locus_tag="PC471_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III"
                     /protein_id="HDF2790012.1"
                     /translation="MKQDKQNEKQSEISKIKQSNDNKLEIAQELHVSKNIGNMLGYRI
                     DKKKMNRPS"
     gene            7310..8578
                     /locus_tag="PC471_002903"
     CDS             7310..8578
                     /locus_tag="PC471_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HDF2790013.1"
                     /translation="MEASIHTIASNNLLIIFAIVSITGIICSKLSEIIKVPDVVLYLL
                     VGILIGPSFLKFIDIRGFQIENQLILTFGSAFILYLGGKEISLKVLRNVKISVFLLAT
                     LGVVISAFIMQQVIGFTFGISAMTALLAGTIIASTDPATLVPIFNQVKIKDRVKQTVI
                     SESAFNDATGAILTSAVLAVILSNKFSLGENIYELGTMVIVGVLVGLITGILLLKLVN
                     DKPYGIFKDFAPIISVISVVVAYELSTKLGGSGYMSCFIVGIITGNKKNFKIWLSQKS
                     YDADFYVAETLGTICRMAIFIILGSQVNLAQLSKYFMPSVITVLVLMFIARPLCVLVC
                     TLVDREAKWSKSEILFMMWVRETGVIPAALCGIISAMKVPGYEVISSVVFMTILITLV
                     IQGSTTKLVAKKLGLLEEEVINISEKVSTF"
     gene            8773..9561
                     /locus_tag="PC471_002904"
     CDS             8773..9561
                     /locus_tag="PC471_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HDF2790014.1"
                     /translation="MFTVMDIVQPDTVEEAYSILNKRKTNQVIGGSAFLRMGKKRIGT
                     GIELSKLNLDYIKEYEDYVEIGSMTTFRTLETSSIIKNNFGRIIEDSVKNIIGVQFRN
                     VVTVGATVFSKYGFSDLIVALLSLDTEVELYNIGRISLEEFLNRDYEKDLLIKIYIKK
                     TNKNASYKSIRNAKSDYPILNVSVSKHMEQFKLCVGARPQKATIAKQASEFLSNNEIN
                     EINIDKAVEIASEELTFGSNMRASREYRKAMSKVLLKRAIMEVI"
     gene            9561..10010
                     /locus_tag="PC471_002905"
     CDS             9561..10010
                     /locus_tag="PC471_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster binding
                     domain-containing protein"
                     /protein_id="HDF2790015.1"
                     /translation="MLVKININGKKKSVDIEPEEYLVDTLRKVGNLSVKRGCDTGCCG
                     LCTVLIDKKPTLSCATLTLRALDKEITTIEGLENEVKEFSEVLVKEGAEQCGFCSPGF
                     ILTVLAMKDELENPTDDEIKHYLTGNLCRCTGYMGQLRAIKTYLEVK"
     gene            10014..12278
                     /locus_tag="PC471_002906"
     CDS             10014..12278
                     /locus_tag="PC471_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HDF2790016.1"
                     /translation="MNIVGKGIKKIDGMSITTGKPVYTDDLSAKDALVVKILRSPHAY
                     AKIKDIDTSKAEMVDGVECVLTYKDVPKSRFTLAGQTYPEPSPYDRLILEDVVRYVGD
                     EVAIVAAIDEKTAIKAMNLIKVKYEVLTPVLDFEEAIGNKTMVHDEDRHCNFDIGMQR
                     EKNIVSHHDYTKGDVEKVLSNCDVVIEETYYTQAQAHAMMETYRAYNYLDHMGRLVVV
                     SSTQIPFHVKRHLSRALEIPASKIRVIKPRIGGGFGGKQTACVEIFSAIVTLKTGKPA
                     KIIYDRKETFNCSTSRHAMRLKVRLGATNDGIIKAIDIKALSDTGAYGEHASTTIGLV
                     GEKTLPMYNKVEATRFIADVVYTNKMPAGALRGYGASQGCFAVESTINMLANKLKMDP
                     TDLRLKNVVTEGETTFAYGKTLNSVDLIKCINKGKELIGWNEKYPCKHLDNGKIRSVG
                     MAITMQGSGIQGLDKSSAEIKLNDCGDYTLMIGSTDMGTGSDTILAQMAAEVLDTSIE
                     NITVISADTDLVPYDPGSYASSTTYVTGMAVVKAAQDLREKIISAGATKLGVEKDEVE
                     FDGIKLTSKKGSTLLFDIAVDSTIGPDTNQLIGLASHGSPVSPPPFIAGFAETEIDTE
                     TGKVDLVNYVAVVDCGTVINKNLAKIQVEGGIVQGIGMALFEEVRYTEKGSMDTNTFM
                     QYKIPARCDIGNVKVDFVETYEPTGPFGAKSVGEVVINTPLPAIQEAVFNGIGVRINT
                     LPITPEKVFMEMNK"
     gene            12418..12729
                     /locus_tag="PC471_002907"
     CDS             12418..12729
                     /locus_tag="PC471_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2790017.1"
                     /translation="MAGNLNNMRAVNNFRGDKNILECLVSFEGRSISQRKVRVFFKEK
                     QNQIEIDFAEEEISKLVENVVLNTSYQEMLYDEIEKQLEIDCIGTWMILSKLKDGSRV
                     H"
BASE COUNT         4711 a         1396 c         2188 g         4560 t
ORIGIN      
        1 aaattaatta tataaattaa aataatattc gtaaagaaaa attcatgttt tgtactgtat
       61 aacatataat tttgacaata tgggtcataa gtttctaccg gaataccgta aatattctga
      121 ctatgtatac tattatttag ttaattaaat atttaattaa ctgaattatg aaggatacgg
      181 ctcagcttat caactactgt gtcgtttttt tgtaaaattt aataggaggt gttgttatgc
      241 aaacaacaaa cagtagtact atgcagaaac tttttccaat tttgactaat aaaaatgtcg
      301 acatgaagaa ggaaataatt gcaggagtaa ctactttttt aactatggct tatataatag
      361 ctgtaaatcc aaatatttta tctgagacag gcatgcctgc aggtgcactt gttacaggta
      421 cttgtttagc agcagcattt ggatgtattt taatgggtgt agttgctaac cttccttttg
      481 ctttagcatc aggtatgggt cttaatgcat tttttgcata tacagtagtt ttacaaatgg
      541 gtgttccttg ggaagtagct cttacagcag tatttgtaga aggtattatt tttatagtat
      601 tatcagtaag tggagttcgt gaggctgttg taaatgcgat accaaaaaat atgaaattag
      661 ctgttacagg tggtatagga atatttattg cacttatagg tctagttaac agtggaattg
      721 ttattggaga ccaagccact cttatcaaaa tgggtagatt tacaccagca gtaataataa
      781 cttgtgtagg attaataatt attgctgtat tagataaaaa aagagttaaa ggttctatac
      841 tttttggaat agtagtgagt tcattacttg cttgggcatt tgcttttata aatccagagt
      901 atgcacaaaa attaggtata tatttacctg gtggattatt taaattcgaa agtttagcac
      961 caatagcagg taaaatagat ttaggatatg tactacatcc aacaaatata ggtggttttt
     1021 tagtagtagt ttgtacattt ttatttgttg atttttttga tacagtaggt acattagttg
     1081 gtgtttgttc taaagcaaac atgttagatg aaaaagggaa tgttcctaat gtaggtagag
     1141 ccttacttac agatgcggtt gcaactacag ttggtgcagg acttggagta tctacagtta
     1201 caacttatgt tgagagttca acaggagtta tcgcaggtgg aagaactgga tggacagcaa
     1261 taacagttgg aattttattc ttagctgcaa tgtttttctc tcctgtattt atagcaattc
     1321 catcatgtgc tacagcacca gctttaatat atgttggata tttaatgctt ggtacagtta
     1381 aggatataga gtttgataat ataactgaag gtgttccagc ttttgttact attgcttgta
     1441 tggcattaac atatagtata ggtgatggtt taactttagg tatattaact tatgttttcg
     1501 taaatatatt ttataatata tttggagcta aaaaggcaga agacaaaaaa catgtttctg
     1561 tagtaatgat tgttctggca atattattta taataaaact ttggctacta taataggtat
     1621 tatttaaaat tttaatttaa tataaaataa agggctgtaa tttattatca attgtgatga
     1681 taaactacag ctcttttgta tttgttgtat ttttgtattt attgtattat ttaataatgt
     1741 ctattagttc tccattttgt aaagttttag gtgtaattac tatattgaac tctttttgca
     1801 aatcttttat aatatctaat tctttttttg ttattattga gattgtgtta ccacttcgat
     1861 ttcctctagc agttcgacca catctatgca ggtattcatt tttacttttt ggaaaatcca
     1921 agttaaaaac atgacttaca tccacaatat ctagtccacg agcagataaa tcagttgtaa
     1981 ttaaaatctt tgctttacca agtttaaatt tatttattgc attctttcta tcttcttttt
     2041 ccatatttcc aaaaattcct attgctttgt aattatgata atttagtttt gatactagga
     2101 cttctatatt tttttcatta tttacaaata caatagcttt ttttggattt gtagctgcta
     2161 atgcctttct aagaaaagta aatttatccc ttatctcacc taataaatat gagtgattga
     2221 tgttaggatt tatttgtgat ttttcctttg tttttattat ttcaaactcc ttcataagtt
     2281 tatcacaaat ttttatagtg ctatcagtaa gacttgcaga gcatcctata atttgtctat
     2341 ctcttaacgt tgttcttatt atgtcttcta tacaagttat attttttcca ttcaatagat
     2401 tgtccacttc atctaagata atagttttta tattatgtga ttttagtttc ttttgtttta
     2461 tcaagtctaa aactcttcca caagagccaa ttactatgtg tggttttact gcttttatat
     2521 tttttatttg tttttgtata ttgacctccc caattaaagc taaagaagta acggagagtt
     2581 ctgcattttt agccaataat tcaacctggt ttgtaatctg cattactaat tcatgcgtag
     2641 gtgcaagtat aagagcttgt gtttctcttt ttgatgtatc aattttttca aatataggta
     2701 aaaggtatgc tagtgttttt cctgttccag tctgtgaatt aatcaataaa tccttatttt
     2761 gaattatatt ccctataaca agtgactgaa cttctgttgg agaagttatg tcttgttttt
     2821 tgagtccatc tattagagtg gaacttattt ttaattgttc aaatgtattc atattttact
     2881 ccttatattt taataattat agtcaactat tttttattat acattgttta ttcaaatcga
     2941 actatatatt cttttataat tatgtgtaat ttgaaagtaa atatcaatta tatttatacc
     3001 tttatatttc tgataccttt gcattttata gatttctaaa ataaacaaat aaaagttgta
     3061 attgtttgat atttgttata tattatatat aacaaatatt aaaactataa tatggaggga
     3121 aattatgtta aaagtaatca atgtatctaa atcatataaa aataaaaaag tagtaagtag
     3181 tgtctcattt gatgtaaatg atggagagct ttttgggttt ataggacata atggagcagg
     3241 taaaactact actattaaag caattgttgg aatacacgat tttgaagagg gagaggtact
     3301 aataaattct aaatctataa aaaaacagcc tgttgaatgt aaaaaggaaa tggcatatat
     3361 acctgataat ccagatttat acgagtcatt aactggtata caatacctta attttatagc
     3421 tgacatattt gaagttgcca aagataaaag agaagagtta ataaaatatt attcaaataa
     3481 gtttgaactc aataaagcat taggagattt aatatcttca tattcacatg gtatgaaaca
     3541 aaaattagct ataatttcgg ctttgataca cagcccaaaa atcttaattc ttgatgaacc
     3601 ttttgtaggt cttgacccaa aagcttcctt tatattgaag gaaattatga gggagttttg
     3661 tgacaaaggt ggttgtatat ttttctcaac acacgtttta gaagttgctg aaaaaatttg
     3721 tgataagatt gccataataa aagatggaaa tataattgct tatggaacta cagaagaagt
     3781 taagggaagt aattcacttg aaagtatctt tatggaatta atagagaaat aaaatgtttt
     3841 ttatacaata tagtgtctaa agtgaggaat gataaaatat gagcaatcta agtatattaa
     3901 taaaaactaa tataataaat gaatttaaat taaatgcgtt aaaaaaagct gatggtagag
     3961 aaaaacaaaa aataatggga atgatgcttt cagttgttat aatagcgggt ctattaattt
     4021 tttatgtaac aagcttaagt ctctctttag ttgatatctt aaagcaaata aatcaactgg
     4081 aaatgctact gatatttggt tttggattat caacattaac aactattgtt acatcattat
     4141 ataaaacgtc atcatatcta tttcaagcaa aagatttaga ccttttgact agtttaccta
     4201 taaaagaatc tactgtacta gcaagtaaga tattaatgtt ggttggagca aactatttat
     4261 tttcttcaat atgtatgatt atacctgcag tggtttactt tttaaattct gatgtaagta
     4321 taacttattt cccttatttg atattgctat ttttagcttt accattatta ccgatagttg
     4381 cttcatcaat aatttttcta tttatagggc agatatcatc aagaataaaa tacaaaaatt
     4441 tagtattaat cataggtagc attatattga tgttgcttta taccctactt atgtcaagac
     4501 ttaattctat gtcagttgaa atgctaaaga atagtgtgtc tatttcagag acaattagaa
     4561 aagtatatcc tcctatatat tattttgttg atgctcttaa aacaggaagt atagtttcat
     4621 ttttagcatt tatagccatc tcaataatac cattttttat gtttgtaact ctatttgcaa
     4681 aaaaatttaa agatataaat gctaagatga gtgaaggata taagtcaaaa aattatgaat
     4741 ttaagggtca aaaaacttca agaccttcaa aagctttatt aaaaaaagaa tttaagagat
     4801 atacttcttc gtttatatac tttttaaatt catctgcagg tttgttattt ctaatggtag
     4861 gtacaatttt aatattatta tttggagcag ataaagtggg tgagttactg aaattgaata
     4921 tagattttaa tcttataaaa atacaattat tgggagtatt cttctttata atagtaatga
     4981 attgtacaac atactgttca atttctcttg aaggaaaaaa cttatggata ctgaaatctt
     5041 ctccaataga tgaaagagaa atattctggt caaaaatatt gatgaatttt ttattgaatg
     5101 caccactatc tattttatgt ctaatattag tatctataag attagacttt gaaattcagt
     5161 ttattattgt atcaatctgt ttgattgtaa gtttttcaat gtttgtagca ttgatgggat
     5221 tattatcaaa tttattatac ccaaaccttg attggaaaaa tgaggtggct gtagtaaaaa
     5281 gaagtgcaag tatgatagtt acaatgttag tatcattagc ttatgtaatt atacttggag
     5341 gtatatatgt attacttgga atttcgtttt taaatatata tattattttg gcaagtatac
     5401 ttactttgat attagatttt atgatatgga gaattatttc tactaaagga gtaagaatat
     5461 ttaaaaattt ataggtgtat ttatgtcaaa taaagactta tcttttttag tcttgcaaat
     5521 aataggttaa gtagtatact atgtatagtg atatgaaaaa atttaggaga aaataaatga
     5581 gaaaattatc caaaagagaa aaagatttta ttcaatatgg atttttatta tttttgatat
     5641 gtattactac ttatttagta ttaactactt tagatataaa actaatacct aaaattataa
     5701 ggctagtaaa taagacttat atatttcttg gcatattgac aattatagta tatatgataa
     5761 ttgaatctat tgtaactcat ttgataataa atgcagttca aaaaacaaaa gtaaaattta
     5821 ttggtattaa aatggctaca atgggatttt actataactt agttactcca tttgcatctg
     5881 ggagtcaacc aatgcaaata tatgcactta caaaatatga tgtaagcctt agtaaatcag
     5941 tggctatagt tactaataaa actgtagtat ttcaaagtac agttactgta ttttgtggaa
     6001 ttttaatttt gctaaataag tcattactga tggagcaagt acattctgtg aaagtcttga
     6061 taacttttgg aatgattatg aatgtaagta tgttgttagg tggactacta atagtattta
     6121 gtccaaaaca agtaaaaatg atagcagaag ttttgattaa ttttttatct aaatttaaaa
     6181 catttaaatt tttagattca aaacgtgata gaataaatca ttatattgat gattataatt
     6241 attctataaa aatatttatt aaaaatgtaa gaatcttagt tttaagtata ttacttactg
     6301 tgttacaatt aatcgcatat ttcagtatag tttattgtgt gtataaagcc tcaaatttaa
     6361 gaggagtttc atatgtacat ataatgacat tacaggtctt tttatatatg gctatatcac
     6421 caattccaac accaggaaat gttggagcta atgaggttac attttttact atgttctcca
     6481 atataattcc aaaagaatta ttggggtact ctgtactctt atatagtggg tttgtatact
     6541 attttatatt aatagtttca ggtttattta catttggaac gcattataca ttaaattctt
     6601 ggaaaaagga aaataaagta aattctaatt tagataaggt tattgaaaaa ttaaattaat
     6661 aaaataatta gggaaattct atatataaat atggtgttga ataagctata ttatattgag
     6721 atatttccct tttttgatta aaattataga aatttgaaat aataagtaat aaacagaaaa
     6781 agtgatattg ttttgaaagg agaaactatg aaacaagata aacaaaatga aaaacaaagt
     6841 gagatttcta agataaaaca aagcaatgat aataaacttg aaatagctca agaattacat
     6901 gtaagtaaaa atatagggaa tatgcttgga tatagaatag ataagaaaaa aatgaataga
     6961 ccaagttaat gaaaatgttg aaaatggtac attttaaaaa atatatataa tgaaatgttt
     7021 tattgttaaa tttaaactta ttttaatggg ggaaaaagtc gaaaaatatc taaaaagata
     7081 tggatataaa aaatattaat atatatatac tataaacttg acaaaatcaa ccttttaata
     7141 actagcaaaa aatagcaaaa ttatataagc gttatttaat tgtcaaaaat gaaagctaaa
     7201 ttgcatgtcc taaaaaaaat gattgataac atttatttgt gtgctataat tatatagtaa
     7261 aacaataagt atattgcata atgtatgcaa atcgggagga gagacggaca tggaagcatc
     7321 tatacacaca attgccagca ataatttatt gataattttt gctattgtaa gtataactgg
     7381 aataatttgt agtaagctta gtgaaataat aaaggttcca gatgtagtgt tatatttact
     7441 tgtaggtatt ttaataggac catcattttt aaaatttata gatattcgtg gatttcaaat
     7501 agaaaatcaa ctaatactta catttggttc tgcatttatt ttatatttag gtggaaaaga
     7561 aataagttta aaagtattga gaaatgtgaa gatatctgta ttcttattag ctactttagg
     7621 agttgtgata tctgctttta taatgcaaca agtaatagga tttacttttg ggataagtgc
     7681 aatgactgct ttactagcag gaactataat agcatcaaca gacccagcca ctcttgtacc
     7741 tatatttaat caggtaaaga taaaggatag ggtaaaacaa actgtaataa gtgaatcagc
     7801 ttttaatgat gcaacaggag ctatactaac atcagcagtg ttggctgtaa ttttgtcaaa
     7861 taaattttca ttaggtgaaa atatttatga actggggaca atggttattg tcggagtttt
     7921 agttggttta attacaggaa tactgctttt gaaattagtt aatgataaac cttatggaat
     7981 atttaaagat tttgcaccaa taatatctgt tatttcagtt gtagttgcat atgaattatc
     8041 tacaaaatta ggtggaagtg gatatatgtc ttgctttatt gtaggtataa taactggtaa
     8101 caagaaaaat tttaaaattt ggttaagtca aaaatcttat gatgcagatt tttatgttgc
     8161 tgaaacttta ggtactattt gtcgtatggc aatatttata atattgggta gccaagttaa
     8221 tttggctcag ttaagtaaat attttatgcc atctgtaatt acagtattag tattaatgtt
     8281 tatagcaaga ccactttgcg ttttagtttg tacacttgta gacagggaag caaaatggag
     8341 taaaagtgaa atattattta tgatgtgggt aagggaaaca ggcgtaatac cagcagcact
     8401 ttgtggtatt atttcagcaa tgaaagttcc tggatacgaa gtgatttcat ctgtagtatt
     8461 catgactata ctaattacat tagtaataca aggaagtact acaaaattag tagctaaaaa
     8521 acttggatta ttagaagaag aagtgataaa tataagtgaa aaagtatcaa ctttttaaag
     8581 ttttgcttta aaatagtacc tgttaatggc tattttaata ttgtctaatg atgattattg
     8641 aaatagtcat ttttttatgt ataagtaatt tacagaattt acatattaga catatttatt
     8701 atcaaaattt gttgaagcta tgatataatt agttgtattt aaaataagaa tagcaaataa
     8761 gggaggaaat ttatgtttac ggttatggat attgtacagc cagatacagt agaagaagcg
     8821 tactcaattt taaataaaag aaaaactaac caagtaattg gtggaagtgc ttttttaaga
     8881 atggggaaaa aaagaatagg aacaggaata gaattatcta aattaaattt agattatata
     8941 aaagagtatg aagattacgt tgagattggt tctatgacta cttttagaac attagaaaca
     9001 agttccataa taaaaaataa ttttggaaga ataatagaag attcagttaa aaatataata
     9061 ggagttcagt ttagaaatgt agtaacagtt ggtgcaacag tattttctaa atatggtttt
     9121 tctgacttga tagtagcatt actttcatta gatacggaag tagaattata taatattggg
     9181 agaattagtc ttgaagagtt tctaaataga gattatgaga aagatttact tattaagata
     9241 tacataaaga agacaaataa gaatgcttct tataaatcaa ttagaaatgc aaagagtgat
     9301 tatccaattc taaatgtatc tgtatcaaaa catatggaac aatttaagtt atgtgtagga
     9361 gcaagaccgc agaaagctac gattgcaaaa caagctagtg aatttctatc taacaatgaa
     9421 atcaatgaaa ttaatataga taaagcagta gaaatagcat cagaagaact aacttttggt
     9481 tctaacatga gagcatctag ggagtataga aaagctatga gtaaagtact tttaaaaaga
     9541 gctataatgg aggttattta atgttagtaa agattaatat aaatgggaag aaaaaaagtg
     9601 ttgatataga accagaagaa tatctggttg atactttaag aaaagttggt aatttaagtg
     9661 tgaaaagagg ttgtgacaca ggttgttgtg gtttatgtac agttttaatt gacaagaaac
     9721 ctacattatc ttgtgcaaca ctgacattga gagctttaga taaagaaata actacaattg
     9781 aaggtttaga aaatgaagtt aaagaattta gtgaagtttt ggtaaaagaa ggagctgagc
     9841 aatgtggttt ctgttctcct ggctttatac ttactgtact tgcaatgaag gatgagcttg
     9901 aaaatcctac agatgatgaa ataaagcatt atcttacagg aaatctatgt agatgtacag
     9961 ggtatatggg acagttaaga gctatcaaaa cttatctgga ggtaaaatag aacatgaata
    10021 tagttggaaa aggaatcaag aaaatagatg gaatgagtat cacaacagga aagcctgtat
    10081 atacagatga tttatcagca aaagatgctt tggtggtaaa gatacttaga agtcctcacg
    10141 cttatgcaaa aataaaagat atagatacat caaaggcaga aatggttgat ggtgttgaat
    10201 gtgttttaac atataaagat gtaccaaaat ctcgatttac acttgctggt caaacatatc
    10261 cagagccatc tccatatgat agattaattc ttgaagatgt tgtaagatat gtaggtgacg
    10321 aagtagctat agtggcagct atagatgaaa aaactgcaat taaagcaatg aatcttatca
    10381 aagtaaagta tgaagtactt acacctgttt tggattttga agaggcaata ggaaataaaa
    10441 ctatggtaca tgatgaagat agacattgta actttgatat aggtatgcaa agagaaaaaa
    10501 atatagtatc ccatcatgat tatacaaaag gtgatgtaga aaaagtatta agtaattgtg
    10561 atgtagtgat tgaagaaact tattatactc aagctcaagc acatgcaatg atggagacgt
    10621 atagggcata caattattta gaccatatgg gaagattggt tgtagtaagt tcaactcaaa
    10681 taccatttca tgtaaaaaga catttatctc gtgcactaga aataccagct agcaaaatta
    10741 gggttataaa acctagaatt ggtggcggat ttggtggtaa acaaactgca tgtgttgaga
    10801 ttttttctgc aattgttact ttaaaaacag ggaaaccagc taaaataatt tatgatagaa
    10861 aagaaacttt taattgttca acaagtagac atgcaatgag attaaaagtt agactaggtg
    10921 ctacaaatga tggcataata aaggcaatag atataaaagc actttctgat acaggagcat
    10981 atggagagca tgcttctaca actattggtt tggttggaga aaaaacttta ccaatgtata
    11041 ataaagttga agcaacaaga tttattgcag atgtggtata tactaataag atgccagcag
    11101 gagcacttag agggtatgga gcatcacaag gatgttttgc tgttgaatca actataaata
    11161 tgcttgctaa taaattaaaa atggacccaa cagatttaag gttaaaaaat gtggtaacag
    11221 aaggtgaaac tacatttgca tatggtaaaa ctttaaatag tgtcgattta ataaaatgta
    11281 taaataaagg caaagaactt attggatgga atgaaaaata tccttgtaaa catcttgata
    11341 atggaaaaat tagaagtgtg ggtatggcaa ttactatgca aggttctggt atacaaggtc
    11401 ttgataagtc ttcagctgaa ataaaattga atgactgtgg agattatact cttatgatag
    11461 gttctactga tatgggtaca ggaagtgata caattttagc tcaaatggct gctgaggttc
    11521 ttgatacaag cattgaaaat ataacagtta tatcagctga tactgactta gttccatatg
    11581 acccaggttc atatgcttca agtactactt atgtaacagg tatggcagta gttaaggcag
    11641 ctcaagactt aagagaaaaa ataatttctg ctggagcaac taaactaggt gttgagaagg
    11701 atgaagtaga atttgatggt ataaaattaa ctagtaaaaa aggtagcact ttattattcg
    11761 atatagcagt tgattctaca attggtccag atactaatca attaataggt cttgcttctc
    11821 atggtagccc agtatctcct ccacctttta tagcaggttt tgcagaaact gaaattgaca
    11881 cagaaactgg aaaagttgat ttagtaaatt atgtagctgt agtagattgt ggaacggtta
    11941 taaataaaaa tctagctaag atacaagttg aaggtggaat tgttcaaggg attggaatgg
    12001 cattatttga agaagttaga tacacagaaa aaggaagcat ggacacaaat acatttatgc
    12061 aatacaagat tccagctaga tgtgatattg gaaatgttaa agtagatttt gttgaaactt
    12121 atgaaccaac tggaccattt ggtgctaaat ctgttggaga agttgttata aatacacctt
    12181 taccagcaat acaagaagct gtgtttaatg gaattggtgt aagaataaat acactcccta
    12241 ttacacctga aaaagttttt atggaaatga ataaataatt atttatgaaa atttagacta
    12301 gttttacatg ttaaacaacc agttatgtca tacctgttga aagatattta ataagtagtt
    12361 attataatat tatgaatttt acttataaaa tacaaaatta aataagggga tgatgttttg
    12421 gctggtaatc taaataatat gagagcagta aataatttta gaggagataa gaacatttta
    12481 gaatgtttag tcagctttga gggtcgttca ataagtcaga gaaaagtaag ggtatttttt
    12541 aaagaaaaac aaaatcaaat agaaattgat tttgcagaag aggaaatttc taaattggtt
    12601 gaaaatgttg ttttaaatac atcatatcaa gaaatgttat atgatgaaat agagaaacaa
    12661 ctggaaattg attgtatagg tacttggatg atattatcta aattaaaaga tggtagtaga
    12721 gttcactgat tttaaaataa attattagag cctaatttta aggctctttt ttgctgtttt
    12781 taattaacaa tagaaaattt atctttagtt tgtatataat tttaatatca tatattctat
    12841 atatataaaa ttgaa
//
LOCUS       DAMJYR010000109        12698 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.112, whole genome shotgun sequence.
ACCESSION   DAMJYR010000109 DAMJYR010000000
VERSION     DAMJYR010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12698)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12698)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12698
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.112"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            <1..276
                     /locus_tag="PC471_002908"
     CDS             <1..276
                     /locus_tag="PC471_002908"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2790018.1"
                     /translation="KLYNFYLLDFDEFYNELSKKKKLYFKNIDELEEYFNKYKDAINR
                     IIYDINVCENTINTFVYDLYGIIELESKSIEDICKTTKDTVVTSLAY"
     gene            complement(372..821)
                     /locus_tag="PC471_002909"
     CDS             complement(372..821)
                     /locus_tag="PC471_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2790019.1"
                     /translation="MFGERLKKLRIKFGLKQHELAEILNVSQSTIGMYENDQRTPPAE
                     SIVKLAEYFNVTTDYLLGHTKTNYSVSANIPGMPSIVCEDNSIYDILDGKKDIKSLED
                     MNKFLENTDYNNEVKEVLKKYMQLDEMDRKAIERMIDNAYERLKEDN"
     gene            1082..2125
                     /locus_tag="PC471_002910"
     CDS             1082..2125
                     /locus_tag="PC471_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor KilAC domain-containing
                     protein"
                     /protein_id="HDF2790020.1"
                     /translation="MEIINRENEKRIISFNNELFGEIRTIRIENEPYFVATDIAKALG
                     YKDTTNAIKQHCRWVVKHHIPHPQSKTKTLEVNIIPEGDMYRLIINSELPSAEKFERW
                     VFDEVLPSIREHGAYMTNEALEKAINDPDWTIKLLTELKKERATKEKLKVEQEKNKPK
                     LELANAIESSSSSILIAQLSKILNQNGVDIGQNRLFEWMRNNEYLIRKKRADHNTPTQ
                     KSMDLKVLEVSESTGIDKDGNTVIRQTPVVTGKGQIYFVNKILKENNKVLKRFMINND
                     YIRGVMVEIGKTYAIYDHEYYGFIGILKSVRNEKIRVEKIGDVSSSRDIPICMIDYIE
                     EASKEDINKYLSK"
     gene            2226..3386
                     /locus_tag="PC471_002911"
     CDS             2226..3386
                     /locus_tag="PC471_002911"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2790021.1"
                     /translation="MLISEYIKDNRCGKKSKLYFKKLEEIKQEFINRDLEYLLLTTWD
                     EEISCMMKTGRDLDKLKYRDGFKEINNLFDSISEEIRDEYKEKDTYTMFFSSCSKEKY
                     YIACELANSAYKHIILPNIFEDDKSVNHKVVFKKEQKKEIALMLKLISSYMQHGFSGY
                     KIYDTDIKFIENLCLFEGKKVCYPKPNNLTHFLGDISRSDNDPIEILHARNVINKIME
                     KGYFDNEFELVYFLRGKIMTPMQNKYEKIDTSSLEKINYLEEKALIQKKMILNDEMAY
                     KWKSEINMFKVICKKFKDATHQYRPEWLNGQTLDVFVPSINLAFEYQGQQHFKSVDFF
                     GGEETLEKNKLRDKKKKRLCDENNVKLIYWNYDEPITTIVLKKKLKENNVEL"
     gene            3446..4156
                     /locus_tag="PC471_002912"
     CDS             3446..4156
                     /locus_tag="PC471_002912"
                     /inference="COORDINATES: protein motif:HMM:NF021378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HDF2790022.1"
                     /translation="MEEYLGHFILIGLGVGYIIVYCFIRNVMDNAKEKEARLERIAER
                     NREKTKVEKAIQKNISMAKCQAFGNSNSDSVILSNNGKISIIHAGFKTAVKKNIEDLV
                     SIKFSMQVSEKNQMRIISIVPTYDKYTFVEKIYLTLIFELNTYEVFYIPQYKNINNAD
                     IQEKIKEMERFKLVVENEAAKFKNAKIESNNDIKKDDDDISKISNSLKELQSLKDQEI
                     LTEEEFNEKKKVLLEKIK"
     gene            4215..6098
                     /locus_tag="PC471_002913"
     CDS             4215..6098
                     /locus_tag="PC471_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HDF2790023.1"
                     /translation="MAEEFKIKTSIELDDKKARKQLASLKTSAKENNIKLNVEMNASS
                     LNNLKQLENTLKQINKLSRETQNGLFGGKGNSNGNINKLTSQYDNFRKKIESTQRQLK
                     KFTQTNILDNKQIGEINKLSGELKRLSNIKLGGLNSKALSDLSNVQSKLANMKIPDMN
                     TKMASQFKTLQNEAGKLANKIETLGKSGYADTSKLQALSNSLKSIQNLNLKNLTSNQI
                     QSEIQKLEQLKNKVKEVENATKNTKLDAKFNMNLSKVISDLNRLRQKCLELGQSTAGI
                     DKLEKELMQLNGMPLGQKVKELDSIKSRLSNMKSNFTGLGTSVKATNGFFSDLYNSMR
                     TYTLGNMIGMSITNGVRNIKTTIVELDSALRDMMKVAPDNFEGTSEQLKNVKNEAISI
                     GKDTARASEDIIQGASKALQTGVKSVSDSLKIAKQSAVFANVGDLDQETADKYLTSVM
                     SAYGGMTKALKPIKDTRVQIKGMSKDYDNLTKFLDLSNYAGNNFAITTGDVGAALQRS
                     ASMLSSAGVSMEDSVALIVGGNESVQNAEKVGTALKTIGINMSGIKASADSGKISLNK
                     TAKTLQETAKINVLDKQTGQVRDMMSILDELAEKWHSVGEDALTKNQKSGIAEAINFS
                     RLI"
     gene            6408..12611
                     /locus_tag="PC471_002914"
     CDS             6408..12611
                     /locus_tag="PC471_002914"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2790024.1"
                     /translation="MDNWNQVKKFQSAWLGGEVLGSASKENERFINSAEGKIIKLKES
                     LKQLVTDTISTDMFKTSLDGLSGITGILNGITKAADKMHVSLPVALAGLSSLFMTIKA
                     LGTGKPITNLGGSIFSAFQNNRKSVKFKDFERATKFTNQYTSAINKNTNASKNNAKSN
                     QVVNRTINSQNGIVGKYTSTTNSLKKATAKEIKINEDRTKSYEKYNQKLKVVSKSRKD
                     TISDGLGNIGKAFAGSSIVNFGKGIATTVGNSLVLTAAFAGVSLLAQAMENYANREEN
                     AYQARKKNIQASKQQINSYESQKVQLQALAEEYDNLSKKENKSKEDNNRLNELKQQIA
                     KIKPDAVIGTDENGIPILKGQVTDLIAEIDRAINAKERLMSYDKHDNAKTAAKKLNSP
                     SKDVNKTLEERMRESETGKLVKIEEDYTAEVEKNKKRQEKAIEKYNNSTGRARDKARN
                     ELMSAKAEEEKIYFKYDEMYRNQVDKIQGYSKEIGDGIFSNIKNKTLYSGLEGNDKSN
                     FAGLESLFDFSEVTPDTLVDTEQAVNKLLTAVRSGKVDVGDLSKTLKDANEEFARTQD
                     IEKYNQTIDKTAKSIAKATNTDANIWENLFGQVNPNGIKDMTSINTLLAKFGKTKLDL
                     ANGDKLAMQLQNQFDSIQNILDTTTITGDVKVDANILTDIKNTKEVPSQVKGMIDALL
                     GTGASSTDVLKFTMDVLMELQTGDPDITKLQNDLDNKFGKGKFTITPEILLSNDSGQA
                     NAEQIISSLKQRYEELPEEVITVIKANPTATLEEADSVKRIYDKFPKEVKTIIKEEGA
                     DEGGRKILDLTSKYAEVPSELKTKLEADGVGLEKAVEVSEIYKKVPAELKTYFIAEAG
                     EALYNSVNLKDALEHIPDEKITEIYLKQNDGKLGVQDLITSLDKIPLDKDVRINIYKA
                     LSDGDIDALGKAIESLPPDKRVEIIAEIEKAKDDIETISSSEIANKIFTIEVKDLASD
                     AIEWIQKKLKEINGDKNKKDPVKEAKESTKKVLKDKPNPKSVAFSRDKEAEKLVDDIL
                     NTPPTKQIELKLKKNEELRNTLNSFKQLEGRNDVKLKLESSGIETEQVKIFADIVKNL
                     PTNSTFTNKFITDNVDSLKNLKDYEAVQKWLMDNPTIAMDYSINLNGLDDFDKMKSIY
                     DGLEDKKDKKVFAEVIIKNPEKAEAFQKLYDNVPEETKSKVVSFAVENADELERATKL
                     YESTPDEQKNQVLNFMLNNEDKLYLIEQLYNDFPESKDIIANLIVNNPDALDEVENLN
                     ALDLDKDVKINIIKSLTNGDINSLIAEIEKLPPEKQVEVIAAIEGAIEGIDSVDKKTI
                     KDKWAKLKANNSAALKTIRQTENKKLSDKSFTITAHLRTVGTLGGIASQFANNILGGL
                     KSDRKSVQSINSIETPQAIPANLSAKPRTSEPAPISDETPVTKPSLFSRTVSRATSPV
                     KTLTEKFSRITKTPKIALDTKSIDSAVKYSIELLKELENAISKVTDRISLLDKKMKYA
                     SGKDKLKYLEEQNALYKEELTLLDQKDKALNTQKNRLKDRLKKDYKYTFSDDDNLTSY
                     EEKLISLEKELEKLDKQSESDKGSKKSEERKKKIEEEKKVVEEYLKIAFTEIPKVADA
                     QQEVTNSLIESTRAAEEFKKELKEIAREAALTSAQKHVTEIQNEIDLIDILMKNAEGD
                     EKLDLIEKKKELLAKQAKEIKDVIKVYEDTAKELREGLSKEGFKFSADGKSITNYEQQ
                     LAFLKNNKDADKYKEIAENAKKYLDLLLKDLPDANKQIQQNKEEIEDLNKEIQDAYKD
                     QLKEAQSLQEKIRDMYKKELEERLKEIDKETKAKIDSLKKQQDAYNDSRKEAKYKDDY
                     EEQQDVISDLEKQIAIAERDSSLSGQKKLKDLQKQLKEEQKKLQDLVQDHVDDQVNDM
                     YDKESDRLQEEADKLKEELEKKYSDENLVDLINEAISSGKFVGLDGEVKKLQDAIIEY
                     INKYENGMLAMGSVTKQEWLDKLKEGKETLEDINDILDELDLSKFAMPNYNPSSNSRS
                     RSVSPTSSVNFNSPFFVVQGNVTKDTMKDLEKFGRDLENRVYRKIVENIKS"
BASE COUNT         5474 a         1408 c         2183 g         3633 t
ORIGIN      
        1 aaattatata atttttattt attagatttt gatgaatttt acaatgaact tagcaaaaaa
       61 aagaagcttt attttaagaa tattgatgag ttagaggaat attttaataa gtacaaagat
      121 gctattaata gaatcatata cgatataaat gtatgcgaaa atacaatcaa tacttttgta
      181 tatgatttgt atggtataat tgagctagaa agtaaatcta tagaagatat atgtaagaca
      241 acaaaagaca ctgtggttac aagtttagct tattaatgaa caattagaag gtctacttaa
      301 agtaggcttt ttttgttata attcaatgat gacagaggta tgacaagtat aatttaaaaa
      361 agaggtgtat attaattatc ctcttttagt cgttcatatg cattatctat cattctctct
      421 atagcttttc tatccatttc atctagttgc atatattttt taagaacttc tttgacttca
      481 ttattgtaat cagtattttc taagaattta ttcatatctt ctaaagattt aatgtctttt
      541 ttcccatcaa gtatatcata gatggaatta tcttcacata cgattgatgg cataccaggt
      601 atatttgcac tgactgaata gttggttttt gtatgaccta aaagataatc tgtagtgaca
      661 ttaaagtatt cagctagttt aactatagac tctgctggtg gagttctttg gtcgttttca
      721 tacataccta tagtgctttg tgatacattt aatatttcag ctaattcatg ttgctttaat
      781 ccaaacttaa ttctaagttt ttttaatctt tctccaaaca tttttcacct ccaagatatc
      841 tttataatta ttataccaca taaagtaata aaatattata cttgacatta cattatgtgg
      901 agtgtattat aaaaatataa attactttaa gtgataaaat tacttttagt aattaaaaag
      961 aagccttaca aactttggac ggcgagtaag acttcttaaa tagctgatat aattatcaac
     1021 ttgaatagaa tgattatatc agcttcttat aaaaaataca acttataagg agggattaat
     1081 tatggagata attaatagag aaaatgaaaa aagaataata agctttaaca atgaattatt
     1141 tggagaaata agaacaatta gaattgaaaa tgaaccctat tttgtcgcaa cagatattgc
     1201 aaaagctctt gggtataagg acacaacaaa tgcaattaaa caacattgta ggtgggtggt
     1261 gaaacaccac atacctcacc cacaaagtaa aactaaaact ttggaagtga atataatacc
     1321 agaaggtgat atgtatagat taattataaa tagcgaatta ccaagtgctg aaaaatttga
     1381 gcgttgggta tttgatgaag ttcttccatc aataagagaa catggagctt atatgactaa
     1441 tgaagcatta gagaaggcaa tcaatgaccc agattggact ataaaattac ttacagaact
     1501 aaagaaagag agagctacta aagaaaagtt aaaagtagag caagagaaaa ataaacctaa
     1561 acttgaacta gctaatgcta tagaaagttc atcaagctca atactaattg ctcaactatc
     1621 taagatatta aatcagaatg gagtagatat agggcagaat agattgtttg agtggatgag
     1681 aaataatgaa tatctaatta ggaagaaaag agctgaccat aatactccaa ctcaaaagtc
     1741 aatggatttg aaagtattag aggtttctga gagtactggg atagataaag atggaaatac
     1801 tgtaataaga caaactcctg ttgtaactgg aaaaggacaa atatattttg taaataagat
     1861 attgaaagag aataacaaag tacttaagag attcatgata aataatgatt acataagagg
     1921 agttatggtt gagataggta aaacatatgc tatatatgac catgagtact atggatttat
     1981 tggtatatta aagagtgtaa gaaatgagaa aataagagta gaaaaaatag gagatgtttc
     2041 tagcagtagg gatattccaa tctgtatgat agattacata gaagaagcta gtaaagaaga
     2101 tattaataaa tatttgagta aatgattttg tggaaatagg agttttggct tctatttcct
     2161 tttgtagtat aaattttcct ttagaattga tacaattaat gtatcagaat aaaggagata
     2221 atgttatgtt aataagtgaa tatattaaag ataatagatg tgggaaaaaa agtaaattat
     2281 atttcaaaaa gcttgaagaa ataaaacaag aatttattaa tagagattta gaatatttgt
     2341 tgttaacaac ttgggacgaa gaaatctcat gtatgatgaa aactggaaga gatttggata
     2401 agttaaaata tagggatgga tttaaggaaa taaacaattt atttgatagc atatcagaag
     2461 aaataagaga tgaatataaa gaaaaagata catatacaat gttttttagc agttgctcta
     2521 aagaaaaata ttatatagct tgtgagttag ccaatagtgc ttataaacat ataatacttc
     2581 ctaatatatt tgaagatgac aaaagtgtaa atcacaaagt tgtgtttaaa aaagaacaaa
     2641 aaaaagaaat agcattaatg ttgaaactta tttctagcta tatgcaacat gggttttcag
     2701 gttataaaat ttatgataca gatataaaat ttatagaaaa tctatgtttg tttgagggta
     2761 aaaaggtttg ttatccaaaa ccaaataatt taacacattt tttaggagat atttctaggt
     2821 ctgataatga cccaatagaa atacttcatg ctagaaatgt aataaacaaa ataatggaaa
     2881 aagggtattt tgataatgaa tttgaactag tgtatttttt aagagggaaa ataatgactc
     2941 caatgcaaaa taagtatgag aaaattgata catcatcatt agaaaaaata aattatttag
     3001 aagaaaaagc gcttatccag aaaaaaatga ttttaaatga tgaaatggct tataaatgga
     3061 aaagtgagat taatatgttt aaagtaattt gtaaaaagtt taaagatgca acacatcaat
     3121 atagacctga gtggttaaat ggtcaaactt tagatgtatt tgttccctct attaatttgg
     3181 cttttgagta tcaaggacaa caacatttta agagtgtaga tttttttgga ggggaagaaa
     3241 ctcttgaaaa aaataaatta agagataaaa aaaagaaaag gctatgtgat gaaaataatg
     3301 ttaaattaat ttattggaat tatgatgaac caataactac tatagtatta aagaaaaaat
     3361 taaaagaaaa taatgtcgaa ttgtaataat tttcctaata gtatattctt gatataattg
     3421 aagtatcagg atagggggag atgttatgga agaatattta ggtcatttta ttttaatagg
     3481 gctaggtgta ggatatatta ttgtttattg ctttataaga aacgtaatgg ataatgctaa
     3541 agaaaaagaa gctagattag aaagaatagc agaaagaaat agagaaaaaa caaaggtgga
     3601 aaaggctata caaaaaaata tttcaatggc taaatgtcaa gcttttggta attcaaacag
     3661 tgacagtgtg atattaagca ataatggtaa aatatctatc attcatgctg gatttaagac
     3721 agcagttaaa aaaaatatag aagatttagt aagcattaag tttagcatgc aagttagtga
     3781 aaaaaatcag atgaggataa tatctatagt tcctacatat gataaatata cttttgtaga
     3841 aaaaatatat ttaactttga tatttgaact taatacttat gaagtatttt atattccaca
     3901 atataaaaat ataaataatg cagatattca agaaaaaata aaagaaatgg aaagatttaa
     3961 attagttgta gagaatgaag cagccaaatt taaaaatgct aaaatagaaa gcaataatga
     4021 tataaaaaaa gatgatgatg atatatcaaa aatttcaaat tcattaaaag aattacagag
     4081 tttgaaagac caagagatat taacagaaga agaatttaat gaaaagaaaa aagtattatt
     4141 agagaaaatt aaataaaaat attaagacag tatattaact gtcttttttt atgctttaaa
     4201 aaggagttgg ttaaatggca gaagaattta agattaagac cagtatagag cttgatgata
     4261 aaaaagctag gaaacaatta gcatcactga agacatctgc aaaagaaaat aatataaagt
     4321 taaatgtgga gatgaatgca tcctcattaa ataatttaaa gcaattagag aacacattaa
     4381 aacaaattaa taaacttagt agagaaaccc aaaatggatt atttggtgga aaaggtaatt
     4441 caaatgggaa tataaataaa ttaacttctc aatacgataa ctttaggaaa aaaatagagt
     4501 ctactcaaag acaattaaag aaatttactc agacaaatat tttagacaat aagcaaatag
     4561 gagaaattaa caaattatca ggagaattga aaagattatc taatattaaa ttaggtggtc
     4621 taaattcaaa ggctctatca gatttatcaa atgttcagtc taagttggct aatatgaaaa
     4681 taccagatat gaatacaaaa atggcttctc aatttaaaac tcttcaaaat gaagctggaa
     4741 aattagctaa taaaatagaa acattgggta agtctggata tgcagataca tctaaactac
     4801 aagctttatc aaattcatta aagtcaattc aaaatttgaa tcttaaaaat ctaacttcta
     4861 atcaaataca atcagaaata caaaaactag aacaattaaa aaataaagtt aaagaagtag
     4921 aaaatgctac taaaaatact aaattagatg ctaagttcaa tatgaatctt tccaaagtaa
     4981 tttcagattt aaataggctt agacaaaaat gtttagaatt gggtcaatca actgctggaa
     5041 ttgataaact agaaaaagaa ttaatgcaat taaatggtat gcctctaggt caaaaagtaa
     5101 aggaattaga ttcaataaaa agcagattaa gcaatatgaa atctaatttc acaggattag
     5161 gaactagtgt aaaagcaaca aatggatttt ttagtgacct atataattcg atgagaactt
     5221 atactttagg taatatgata ggtatgtcta ttacaaatgg tgtaagaaat attaaaacaa
     5281 ctatcgttga attagatagt gctttaagag atatgatgaa agtagcacct gataattttg
     5341 aaggtacaag tgagcaatta aagaatgtaa aaaatgaagc aatatcaata gggaaagata
     5401 cagctagagc atcagaggat ataattcagg gggcatctaa agctctacaa actggtgtaa
     5461 aatcggtatc agacagtttg aagatagcta aacaatcagc agtttttgca aatgttggtg
     5521 atttagacca agaaactgct gataagtact taactagtgt tatgtccgct tatggtggaa
     5581 tgacaaaagc attaaaacca attaaagata ctagagttca aatcaaagga atgagtaaag
     5641 attatgataa tcttacaaaa tttcttgatt tatcgaacta tgcaggtaat aattttgcaa
     5701 taacaactgg cgatgtagga gctgcattac aaagaagtgc aagtatgtta tcatcagcag
     5761 gcgtttcaat ggaagactct gttgctttga tagttggagg aaatgaatct gtacaaaatg
     5821 ctgaaaaagt aggtacagca ctcaaaacaa ttggtataaa catgagtggt atcaaagctt
     5881 cagcagattc aggaaaaatc agcctcaata aaacagcaaa aactttacaa gaaacagcaa
     5941 aaataaatgt gttagataag caaactggcc aagtaagaga tatgatgtca attttagatg
     6001 agttggcaga aaagtggcat agtgttggcg aagatgcctt aactaaaaat caaaaaagtg
     6061 gtattgccga agcgattaat tttagtcgcc ttatatagta atatataatg ataaactcct
     6121 ttaattgctg gaaactcccg ttaggctttt agtaccaaag tgtgaaaaac taaaagatag
     6181 ggacaatcag cagccaaaac tcaatgagag taaggttcaa cgactaggtt ataaaccgta
     6241 cactataagc tattgatagt ggaaatgggg agcatcctta taggatgaag atatagtctc
     6301 atctatatgg aaacatatag cagttcataa gagaacgagt taagtatagc gaacttaatt
     6361 gaagattttg ttcaggtaaa aaccacataa atactttcat ggctgtcatg gataattgga
     6421 accaggtaaa aaaattccaa agtgcatggc tgggtggaga agtgctaggt agtgccagta
     6481 aagagaatga gagattcata aattcagcag aaggtaagat tataaaatta aaagaaagtc
     6541 ttaaacaatt agttacagat actatatcta cagatatgtt taagactagt ttagatggat
     6601 tatctggtat tacaggaatt ttaaatggta taactaaagc ggctgacaaa atgcatgttt
     6661 cgcttcctgt agccctagca ggtttatcgt ctttatttat gacaattaaa gcattaggta
     6721 ctggtaaacc aatcacgaat ttaggaggaa gtatatttag tgcctttcaa aacaatagaa
     6781 aatctgttaa atttaaagac tttgaaagag ctacaaaatt tactaatcaa tatacaagtg
     6841 ctataaataa aaatactaat gcttcaaaaa acaatgctaa atcaaatcaa gttgttaata
     6901 gaactataaa tagtcaaaat ggcattgtag gaaaatatac atcaacaacc aacagtttaa
     6961 aaaaggcaac agctaaagaa ataaaaataa atgaagacag aacaaagtca tatgaaaaat
     7021 ataatcaaaa attaaaagtt gtttctaaaa gtagaaaaga tacaataagt gatgggcttg
     7081 gtaatattgg taaggcattt gcagggagta gcattgtaaa ttttggtaaa ggtatagcaa
     7141 ctacagttgg aaattcttta gttttaactg ctgcatttgc aggtgtaagt ctattagcac
     7201 aagctatgga gaattatgca aatagagaag aaaatgctta tcaagctaga aagaaaaata
     7261 ttcaagcttc aaaacaacaa attaattcat atgaaagcca aaaagttcaa ttacaagcac
     7321 ttgcagaaga atatgataat ttatctaaaa aagaaaataa gtctaaagaa gataataata
     7381 gattaaatga actaaaacaa cagattgcaa aaataaaacc agatgcagtt ataggaacag
     7441 atgaaaatgg tatacctatt ttaaaaggtc aggttactga tttaattgct gaaatagata
     7501 gagctattaa tgcaaaagaa agattgatgt cctatgataa acatgataat gcgaaaactg
     7561 ctgctaaaaa attaaattct ccaagtaaag atgtaaataa aactttagaa gaaagaatga
     7621 gagaaagtga aactggtaag cttgtaaaaa tagaagaaga ttatacagca gaagttgaaa
     7681 agaataaaaa aagacaagaa aaagcaatcg agaaatataa taattcaact ggtagagcaa
     7741 gagataaagc cagaaatgaa ttaatgtcag ctaaagcaga agaagaaaaa atatatttca
     7801 aatatgatga aatgtataga aatcaagtag ataaaattca agggtattca aaagaaatag
     7861 gagatggaat attttctaac attaagaata aaactcttta tagtggatta gaaggcaatg
     7921 ataagtctaa ttttgctgga cttgaaagtt tatttgattt tagtgaagtt acaccagata
     7981 ctctagtaga cactgaacag gcagttaaca aattgcttac tgctgttaga agtgggaaag
     8041 ttgatgtagg agatttatct aaaactctta aggatgcaaa tgaagaattt gctagaacac
     8101 aagatataga gaaatataat caaacaatag ataaaacagc taagagtata gctaaagcaa
     8161 ctaacacaga tgctaatata tgggaaaatt tatttgggca agtaaatcca aatggaatta
     8221 aagatatgac atctataaat acattattag ctaagtttgg taaaacaaaa ctagatttag
     8281 caaatggtga taaacttgca atgcaactcc aaaatcaatt tgatagtatt cagaatatat
     8341 tagatacaac aacaataact ggagatgtaa aagttgatgc aaatatatta actgacatta
     8401 agaatactaa agaagttcct agtcaagtta aaggcatgat agatgcttta ttaggaacag
     8461 gggcaagttc tactgatgta ttaaaattca ctatggatgt attaatggaa ttacagactg
     8521 gtgacccaga tataactaaa cttcaaaatg atttagataa taaatttgga aaaggtaagt
     8581 ttactataac tccagaaata cttttaagta atgattcagg tcaagctaat gcagaacaaa
     8641 taataagtag tttaaagcaa agatatgagg agcttccaga agaagtaatc acagtcataa
     8701 aagctaatcc aactgcaaca ttagaagaag ctgattcagt aaaaagaatt tatgataaat
     8761 ttccaaaaga agtaaaaact atcataaaag aagaaggagc agacgaaggc ggtagaaaaa
     8821 tattagattt aacatctaag tatgccgaag taccttctga actcaaaact aaattagaag
     8881 ctgatggagt tggactagaa aaagctgttg aagtatcaga gatatataag aaagtacctg
     8941 cggaacttaa aacatatttt atagcagaag cgggagaggc tttgtataat tctgttaatt
     9001 taaaagatgc cttagaacat atacctgatg agaaaattac agaaatatat ttgaagcaaa
     9061 atgatggtaa attaggggtt caggatttaa taacatcatt ggataaaatt cctttagata
     9121 aggatgtaag aataaatata tacaaagcct tatctgatgg agatatagat gctttaggca
     9181 aagctattga aagtttacca ccagataaac gagtagaaat aattgccgaa atagaaaaag
     9241 ctaaagatga tattgagact attagttctt ctgaaatagc aaataaaatt tttactatag
     9301 aggttaaaga cttagcatca gatgctattg aatggattca aaaaaaatta aaagaaataa
     9361 atggtgataa aaataagaaa gaccctgtta aagaagctaa agaatctact aaaaaggttt
     9421 taaaagacaa accgaatcct aaaagtgttg cattttcaag ggataaagaa gcagaaaagc
     9481 ttgtagatga tatattaaat acacctccaa caaaacaaat tgaacttaag ttaaagaaaa
     9541 atgaagagct tagaaatact ttaaattcat ttaaacaact tgaaggaaga aatgatgtaa
     9601 aattaaaatt agaatcatca ggtattgaga ctgaacaagt taaaattttt gctgatatag
     9661 taaaaaatct tccaacaaat tcaactttta ctaataaatt tataactgat aatgttgata
     9721 gtttaaaaaa tctaaaggac tatgaagccg tacaaaaatg gcttatggat aatccaacta
     9781 tagctatgga ttatagcata aatttaaatg gtttagatga ttttgataaa atgaaatcta
     9841 tatatgatgg attagaagac aaaaaagata aaaaagtatt tgccgaagtt ataattaaaa
     9901 atccagaaaa ggctgaagca tttcaaaaac tatatgataa tgttccagaa gaaacaaaaa
     9961 gcaaggttgt tagctttgct gtagaaaatg cagatgaact tgagagagct acaaaactat
    10021 atgaatcaac accagatgaa caaaaaaatc aagttcttaa ttttatgtta aataatgaag
    10081 ataagttata tttaatagaa caactttata atgatttccc tgaaagcaaa gacattatag
    10141 ctaatcttat agtaaataat ccagatgcat tagatgaagt tgagaattta aatgctttag
    10201 acctagacaa agacgtaaaa ataaatataa taaaatcact aactaatgga gatattaatt
    10261 cattaattgc tgaaatagaa aaattacctc ctgaaaaaca agttgaggta attgcagcta
    10321 ttgaaggagc tattgagggc atagatagtg ttgataaaaa aacaataaaa gacaagtggg
    10381 ctaaacttaa agcgaataat tctgctgctt taaaaacaat tcgtcaaact gaaaataaaa
    10441 aactaagtga taagagtttt acaataacag cacatcttcg tacagtaggg actctaggag
    10501 gaattgcttc tcaatttgca aataatatat taggtggatt aaagagtgat agaaaaagtg
    10561 tgcaatccat aaattctata gaaactccac aagcaattcc agctaatcta tcagccaaac
    10621 ctagaacatc agaaccagca ccaataagtg acgaaactcc tgtaacaaaa ccatctctat
    10681 tctcaagaac agtatcaaga gccacatctc cagtcaaaac tcttacggag aaattctctc
    10741 gtataactaa aactcctaaa atagcattag atacaaaatc tattgattca gcagttaaat
    10801 atagcattga gttactaaaa gaattagaaa atgcgatatc taaagttaca gatagaatat
    10861 ctcttttaga taagaaaatg aaatatgcaa gtggaaaaga taaacttaaa tatcttgaag
    10921 aacaaaacgc tttatataaa gaagaactaa cattgttaga ccaaaaagat aaggcactta
    10981 acactcaaaa gaatagacta aaagatagac ttaaaaaaga ttataagtac actttttcag
    11041 atgatgataa cttaactagt tatgaagaaa aattaatatc attagaaaaa gaattagaga
    11101 aattagataa gcaatcagag agcgacaaag gtagtaaaaa gagtgaagaa cgtaagaaga
    11161 aaatagaaga agagaaaaag gttgttgaag aatatttaaa gattgctttt actgagatac
    11221 cgaaagttgc agatgctcaa caagaagtaa caaattctct tatagaatca actagagcag
    11281 cagaggaatt taaaaaagaa ttaaaagaga ttgctagaga agctgcttta acttcagctc
    11341 aaaaacatgt tactgaaata caaaacgaaa tagacttaat agacattctt atgaaaaatg
    11401 ctgaaggtga tgaaaagtta gacttaattg aaaagaagaa agagttattg gccaaacaag
    11461 ctaaggaaat taaagatgtt attaaagttt atgaagatac tgcaaaagag ttaagagaag
    11521 ggttatcaaa agaagggttt aaattttctg ctgatggtaa aagcataaca aattatgagc
    11581 aacaattagc gtttctaaaa aataataaag atgcagataa atacaaagaa atagcagaaa
    11641 atgctaagaa atatttagat ttacttctta aagatttacc tgatgctaat aaacaaattc
    11701 aacaaaataa agaagaaata gaagatttaa ataaagagat tcaagatgct tataaagacc
    11761 agcttaaaga agctcaaagc ctacaagaaa aaattagaga catgtataaa aaagagcttg
    11821 aagagagact taaagaaata gataaagaaa ctaaagctaa aattgattct cttaaaaagc
    11881 aacaagatgc ttacaatgat tcaagaaaag aagccaaata taaagatgat tatgaagaac
    11941 aacaagatgt aataagtgat ttagagaaac aaatagcaat agctgaaaga gatagttctt
    12001 taagtggtca gaagaaactt aaagatttac aaaaacaatt aaaagaagaa cagaagaaat
    12061 tacaggattt agtacaagac catgtggacg accaagtcaa tgatatgtat gataaggagt
    12121 ctgacagact acaagaagaa gcagataaat taaaagaaga gttagagaaa aaatactcag
    12181 atgaaaattt agttgattta attaatgaag caatttcaag cggtaagttt gtaggtcttg
    12241 atggagaagt taagaaactt caagatgcta ttattgaata cataaataaa tatgaaaatg
    12301 gtatgttggc aatgggttct gtcactaagc aggaatggct cgataagcta aaagaaggta
    12361 aagagacatt agaagatatt aacgacatat tagatgaatt agatttaagt aaatttgcta
    12421 tgcctaatta taatccttct tcaaattctc gttcaagaag tgtgtctcca acatcatcag
    12481 tcaactttaa ttcaccattc tttgttgttc aaggaaatgt aactaaggat actatgaaag
    12541 acttagagaa atttggtaga gatttagaaa atagagtata tagaaaaata gtagaaaata
    12601 ttaaatcata gattatttat ataaacaaag gattttaagt agaaatccag atttctctaa
    12661 taaaatccag attttatttc aaaaaataat atgtatat
//
LOCUS       DAMJYR010000110        12488 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.113, whole genome shotgun sequence.
ACCESSION   DAMJYR010000110 DAMJYR010000000
VERSION     DAMJYR010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12488)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12488)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12488
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.113"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(347..892)
                     /locus_tag="PC471_002915"
     CDS             complement(347..892)
                     /locus_tag="PC471_002915"
                     /inference="COORDINATES: protein motif:HMM:NF012798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDF2790025.1"
                     /translation="MRVEPIENKKDIEKLKRYFRNKGKIRDLTLITLGLNTGFRIEDI
                     LKLRVKDVYKKREINIKESKTGKRIRKPINDSLKEILDIYCKDKPTNEYLFKSQKGEN
                     EAISTTQAYRIIKKASKACRIKLNIGTHSMRKTHGLHIFKDKGIEVAQRALNHANQID
                     TLTYIGVVQKQADNAIMKLNL"
     gene            complement(908..1156)
                     /locus_tag="PC471_002916"
     CDS             complement(908..1156)
                     /locus_tag="PC471_002916"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2790026.1"
                     /translation="MEGTKRSYRKIPKLDLRKVKLADVFEKNKSGVFRVEEADEQLLH
                     ECLDETKSIREEKHLTWQIQFKGKSVYCVFYEHPFWDL"
     gene            complement(1143..2078)
                     /locus_tag="PC471_002917"
     CDS             complement(1143..2078)
                     /locus_tag="PC471_002917"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2790027.1"
                     /translation="MINIIDLRFYLRNDLFTNEELTEITKQITEENVFLYDIAEEKGC
                     HPYTLNKCLQRFEHSSMEVGKFIGEMSELHKEGALTTEELKLKFEIVKEILILIKRSE
                     EAEKILHLLENEKVKTVAEKLYKSRSSVYDILKQMGFKKNKYINVWYHIDNENIAGNV
                     ENTKRDVELVHLYQQRYDNFLKNYYLDSEIIDIDKNILDDIETLSEEFEYETVNDFIS
                     VLLLRGLQNERFKNKENINNKNDNNNIVKMKDVEHEIFFTQGAEMLEEYYKTNLIDVH
                     KISKTEIEEMNPKELMELYHLKNEGKAKEDFYGRD"
     gene            complement(2249..2896)
                     /locus_tag="PC471_002918"
     CDS             complement(2249..2896)
                     /locus_tag="PC471_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003411768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDF2790028.1"
                     /translation="MIIGYERVSTRKQDNSRQERVLENEGIKFDKIYRDKVTGATKER
                     EALNRMLLEVNEGDTVYCESISRLGRNLKDLIDIIDTLVQKGVRVKILKEGIDTGNNT
                     YKLLLGIFGAVAEMERETIQERTQQKIDQLKEDKKNTGEINTKSGKWFGRQKVTKELL
                     ETNKEFIKYYNKILDGSLKKNEVAKILNVGRTTLYEWIKVYEGGEIKRSNRAKKN"
     gene            complement(3486..3854)
                     /locus_tag="PC471_002919"
     CDS             complement(3486..3854)
                     /locus_tag="PC471_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004068822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2790029.1"
                     /translation="MELDFKFIGQRIKIARIKKKLTQEVLSEKINVSPQHVSNIETGN
                     SSVSLPTLVAIANTLGVSVDELLCDTILSSKAVFENELEEVLRDCNEYEIRFITDIVK
                     ASKKSIRDIKLFQSNLNDRY"
     gene            3970..4302
                     /locus_tag="PC471_002920"
     CDS             3970..4302
                     /locus_tag="PC471_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HDF2790030.1"
                     /translation="MDIPKIPQTELKVMKFIWSKNDIVTSKEVAKAMELEYSWKMTTT
                     LTILSRLVIKHFLASERIGRMTHYTILIAKKDYKISETKRFLNDIHSNSLESLLNSLK
                     DCNITHKK"
     gene            complement(4450..4887)
                     /locus_tag="PC471_002921"
     CDS             complement(4450..4887)
                     /locus_tag="PC471_002921"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2790031.1"
                     /translation="MRVKNIISIFVCALIIIMSVNVVSFAEQFNSSDIIFMEDGKEEI
                     NPFSVYILNGRCNLSFSNGKANVNASVVGKSGVTRTSIVTKLQELKGGSWVNLKTSTK
                     KTGGKTCSVLNSYSVTKGHTYRAVAIITAENESKTLISTSQKY"
     gene            complement(4956..5657)
                     /locus_tag="PC471_002922"
     CDS             complement(4956..5657)
                     /locus_tag="PC471_002922"
                     /inference="COORDINATES: protein motif:HMM:NF025643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4367 domain-containing protein"
                     /protein_id="HDF2790032.1"
                     /translation="MNKDKLDIEKLLYEQLPKIEEKILYELPCDEDIDYEFSIEFESK
                     MDKIIKENEKKVKKNTYKNLKKIAIVFIIILSSIVTITTIKTEAFPENLIRIMKKVYK
                     EFTDYNFINPHKSNVSFKYIEPKYISTGYKEVERIEEENYLSILYKDKLDNYIIYQMM
                     LYSRDVIVLDTEDAFVENIKFNNQYAQYVEKGNTQQLLWHDKNNIYMLSKELPTKKIT
                     KKDKKEFIKIAKNIK"
     gene            complement(5650..6195)
                     /locus_tag="PC471_002923"
     CDS             complement(5650..6195)
                     /locus_tag="PC471_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HDF2790033.1"
                     /translation="MLIYLAIIDSKEERIKFEEIYIKYKKLMFYVANQILKDDVLAED
                     AVHSSFLKIIDNLDKVNDVNSPKTKGFVVIIVKRISINIYNRRKKEEISNINDDNYKF
                     SSLDLSIEKLGECSYLGEALKKLSETDLQVILLKYSHGFSIREISKLLNIKEVNLYKR
                     NKRALEKLKKILSEMEALSNE"
     gene            6331..7059
                     /locus_tag="PC471_002924"
     CDS             6331..7059
                     /locus_tag="PC471_002924"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2790034.1"
                     /translation="MKSKRINKMLATTMTMAMLAIPAVTATSYAMESTGLGTEYTYNN
                     ESKQQEEWVKSFDTETGKEILLNKTTNEKVSEVYENVNGKLVKLSINEALEKFNLNEE
                     IITKSPKSIFGVSSVKKITGSARKISQDVSGGKQGATISIGNSTTVTESFTVGGTTEA
                     IKAAVRANAGFTWSKSLTRSVTTTHKVPAGKIGYVAFKPYYKEVRGTVNGILAGTSIT
                     QKIVAKSPLKAGKVCDGLEYLAYK"
     gene            7085..7333
                     /locus_tag="PC471_002925"
     CDS             7085..7333
                     /locus_tag="PC471_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004035436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2790035.1"
                     /translation="MENKNMYLRVSLILAVLGFIILFFNNDLALSLKATYLADKGFED
                     IVENQILKNYSYMFLIIGGVLFSIGIYNLTKLKQINKK"
     gene            complement(7519..10128)
                     /locus_tag="PC471_002926"
     CDS             complement(7519..10128)
                     /locus_tag="PC471_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011572948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HDF2790036.1"
                     /translation="MKIDIKQLIDIIKDANIDELYLRDIGVDTTQKGNVPCPVHGGKD
                     KNFQFDLNKRIYTCYSHGCVVGADIIELCRTYEHFEKPIEAILFLANKYNIPLPFTKN
                     KTNTKKINNVPKNIYIKKKDINSFIEEKKQEAFRNNDIELAFEFECMTDKDKRKYYLD
                     NNIKIKSMLEKQNYSSYKADSIINIDKYISENLKGIRESIKHATEGKNVLMVAPTGSG
                     KTYSIINTLKELDIKALFIFPNSANVQQAIVEYKIAGAYDKIPTKHALEGNKLVAMTW
                     DKTEQLLKEDLSEYIIVIDEIHQTYTDAFRSKAIKSLNNITNKCRGRLDITATPSKLE
                     FEIYNKVIEYKQNIQTEYKVKLYDKINIGKVIEILNNSNNSMLLKDSISTLNYISKKV
                     NKKSGVVISDTKDTSKLYDRIVSYSDMEGYEVLLNTSVITAGVNINNPNVTDIIVIGE
                     KDVGKIKQYVARARGAKSINVHIFNSYKTTNEDSNVYSVEWCINENIKDVESLTNIYN
                     KASKRDLPYRAIGIDISPINIKNIDSNIYYDKKDMCYKTDKLYIKSNAYNNYYQTRTI
                     NSFKVLLEEYFNKIEIVETEKETKKIEEEIKEHEEAIKEFKKSAIEQLEGHKKYLVGY
                     SEIKKSMTSSNLLKYHHDMRIDNNICLKYYMEHDLYSLIINNNCRKTIDLFSDFVLDN
                     SYSIDLSWKLATMSDEKRNLFFEQINTTIYRKINKKYANSLLNDDNIGTYTYNYINFN
                     FGVGTSYTKEHLEALAVDLQTFLATKKKLTIKKISLILQSIFVIEVKQHRGVTGLPYK
                     YYKNILPNPVTDKKVIRVYTIKKHIDIEDIKKELGLIDNDLSIEHLVEARINKLLNAI
                     DETEKEVLLEGLI"
     gene            complement(10421..10723)
                     /locus_tag="PC471_002927"
     CDS             complement(10421..10723)
                     /locus_tag="PC471_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2790037.1"
                     /translation="MLKDDLLRIGFDTYRENVEDELLENDKKYLNDKKESERAMDILL
                     DILEEKEHKKMLIDFEENIFGINVIEKEYAYRMGVLDGLDLAEFIALNKKLCLKRN"
     gene            10892..11095
                     /locus_tag="PC471_002928"
     CDS             10892..11095
                     /locus_tag="PC471_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003366523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2790038.1"
                     /translation="MDIQINKLLENQNKTRYWLAKQIGMTHQNLTKLANNNTNSIKFD
                     SLEKICMALKCSPNELFGWEQDK"
     gene            11175..11720
                     /locus_tag="PC471_002929"
     CDS             11175..11720
                     /locus_tag="PC471_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011572951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2790039.1"
                     /translation="MDRYTFDNLPIKEQIQYINNKLLEGNTLTNTCKDIGIGRTTIRN
                     RFKQHGYEFNPTKKLYISIIEVVELESSSKVVAGDTSNDNTLVDATNIDFNNILSKFN
                     DMNNKLNEVYSWYESQSSNKVVGADKLNIDDFKGNIVVRSYKVYEDIQKEFVAFCEAN
                     KKYRVQDIISQALRDFMDKYK"
BASE COUNT         4116 a         1987 c         1339 g         5046 t
ORIGIN      
        1 aaaaaaaata accatacgca ttgatttcac ttaaattaaa agggtttttc gagttgtttt
       61 ttcacttagt ttagtaattt tgaaatatta taatattaag cttcaatatg catttattat
      121 catttaataa tcaattaata atagccattt tttctaaaca tgataaaaac aaactcaatc
      181 catgtaatag gaatagatag aagtatataa aaaaatgtac gattactata aactattaca
      241 tttattttag ttgccttgat acattgcaat tacttacaca tacgcctatt taattaatca
      301 ttaactataa aatgtaaaaa cagcaaagaa aatcatacat ttttaactat aaattaagct
      361 tcataatggc attatcagct tgtttttgta ctaccccaat ataagttaat gtatctattt
      421 gattagcatg gtttaaagct ctttgtgcta cttcaattcc tttatcctta aatatatgta
      481 aaccatgtgt tttcctcata ctatgagtac ctatatttaa ttttattcta caagcttttg
      541 aagctttttt aataattcta tatgcttgtg tagtgcttat tgcttcattc tctccctttt
      601 gacttttaaa taaatattca ttagttggtt tatctttgca atatatatct aatatttcct
      661 tcaaactatc atttataggc tttcttattc ttttccctgt cttactttct tttatattta
      721 tctctctttt cttgtatacg tctttaactc ttagttttag aatatcctct atcctaaagc
      781 cagtatttaa ccctaaagtt attagggtta aatctcttat tttaccttta tttctaaaat
      841 atcttttaag cttctctata tcttttttat tttcaatggg ctctactctc atttaaacca
      901 ctcctattta taaatcccaa aatggatgtt cataaaaaac acaatataca cttttccctt
      961 tgaattgtat ttgccaagta agatgctttt cttctcttat acttttagtt tcatctaaac
     1021 attcatgtaa aagttgttca tcagcttctt ccactctaaa aacgcccgat ttattctttt
     1081 caaatacatc tgctaatttt acttttctta aatctaattt tggtattttt ctataacttc
     1141 ttttagtccc ttccataaaa atcctcctta gcttttcctt cattttttaa atgatatagt
     1201 tccattaatt cttttggatt catttcttca atttctgttt ttgaaatttt atgaacatct
     1261 attaaatttg ttttataata ttcctctaac atttcagctc cctgtgtaaa aaatatctca
     1321 tgttcaacat ctttcatttt aacaatattg ttgttatcgt tcttattgtt aatattttct
     1381 ttatttttaa atctttcatt ttgaagccct cttaaaagta aaaccgatat aaaatcattt
     1441 actgtttcat attcaaattc ttctgacaaa gtttctatat catcaagaat atttttatct
     1501 atatcaataa tttcactatc taaataatag ttttttaaaa aattatcata tctttgttga
     1561 tacaagtgta ctagctcaac atccctttta gtattctcaa catttcctgc tatattttca
     1621 ttatcaatat gataccaaac attaatatat ttatttttct taaatcccat ttgttttaaa
     1681 atatcatata cacttgacct tgatttatac agtttttctg caactgtctt gaccttctca
     1741 ttttccaata gatgtaatat tttttcagct tcttcagacc tttttataag tattaatatt
     1801 tcttttacaa tttcaaattt taatttcaat tcttctgttg ttaaagctcc ttccttatgt
     1861 aattcactca tttcaccaat aaatttccca acctccattg atgaatgttc aaacctctgt
     1921 aagcatttat ttaaagtgta tggatgacag cctttttcct ctgcaatatc atataaaaac
     1981 acattttctt ctgttatctg cttagttatt tcagttagtt cttcattagt aaataaatca
     2041 tttcttaaat aaaatcttaa atcaattata ttaatcactc ctttttaatt atttttcata
     2101 cattaatctt atcaccaaaa aaattaaatt taaatattcc aagacatcat ctggacgcta
     2161 aaaagtgtcg tttttattaa tttaataata ttatcaaaaa aaagagcaaa gacagtcccc
     2221 taaaataaaa aaagaactag ttataaccct agttcttctt tgctctattg cttcttttta
     2281 tttctccacc ttcatatacc tttatccact cgtaaagcgt agttctacca acatttaata
     2341 ttttcgccac ttcattcttc tttaaacttc catcaagaat tttattataa tattttataa
     2401 attctttgtt ggtttctaaa agctccttcg ttactttttg tctaccgaac cattttccac
     2461 tctttgtatt tatctcgcca gtatttttct tatcctcttt caactggtct attttctgct
     2521 gtgttctctc ttgtattgtt tccctctcca tctctgcaac agctccaaat ataccaagta
     2581 ataacttata agtattgttt cctgtgtcta taccttcttt taatattttt actcttacac
     2641 ctttttgaac caatgtatct ataatatcaa ttaaatcctt taaatttcta cctagacgac
     2701 ttatagactc acaataaacc gtgtccccct catttacttc taacaacatt ctatttaatg
     2761 cttctctttc ttttgtagct cctgtgactt tatccctata tatcttgtca aacttaatac
     2821 cctcattttc taaaactctt tcttgtctgc tattatcttg ttttcttgta cttacccttt
     2881 catatcctat tatcataaca ctacccccgt ttatcttgtt ataataatta tacaatatat
     2941 tttccgttcg gtcaaactat attttctatt ttcacaatac cgaacgtatt ttattgttga
     3001 tttccaatga ttctatgtaa aaataacata aatatcatac gtacggttaa acgttggttt
     3061 ttccgaacac gaacatatca ccttttttat atcaactatt ccttaaatat tttatataaa
     3121 atcgccaaaa tctttttata ttttttaggc aaccaagttt ttaaaacaaa aatggtatat
     3181 cagtaagaat tatgttttca cacaatataa ttcttaatga tataccattt aagaacctca
     3241 taagaggtag atagagataa ttacataatc atcacatacg ttttatcaaa tctgttttgt
     3301 aatagggttt atacttaaat aataacaaaa ataccgtatt ttttctacat ttatactttt
     3361 tttgttgata gtaaaatatg gaaattagtc atattttcag ttattaactc ataagtttat
     3421 acaactttta tttaaatata ataaaaacat caataataat taaattattg aagcatatat
     3481 ttatattaat atctatcatt taaatttgat tgaaacagtt ttatatctct aatggatttt
     3541 ttagaagctt ttacaatatc agttataaat cttatttcat actcattaca atccctcaaa
     3601 acttcttcta actcattttc aaagacagct ttagaactta agattgtatc acataataat
     3661 tcatcaaccg acaccccaag agtatttgca atagcaacga gcgttggcaa acttacactt
     3721 gaatttcccg tttcaatatt gctgacatgt tgtggactaa cattgatttt ttctgataaa
     3781 acttcttgtg ttagcttttt cttgattctt gcaattttta tcctttgacc tataaatttg
     3841 aaatcaagct ccatatgcgt attcccttcc tacaagtatt cacacatatt gtaacctttc
     3901 ccaaagtata ttataataaa ttaatagaat gtttcattat gatagttata gtgtatagaa
     3961 aggaattata tggatatacc aaagatacct caaacagaac tcaaagtcat gaaatttatt
     4021 tggagcaaaa acgacattgt aacatctaaa gaagttgcaa aagctatgga acttgaatat
     4081 agttggaaaa tgacaacaac cctaacaatc ttatcaaggc ttgttattaa acacttttta
     4141 gcatctgaac gaataggcag aatgacacat tatacgatac tcatagcaaa aaaagattat
     4201 aaaatatctg aaacaaaaag atttctaaat gacatacatt caaattcatt ggaaagcctt
     4261 ttaaactctt tgaaagattg caatattaca cataaaaaat agtacatgag tataaattat
     4321 attattatac ctatgtacta tttaagtttt tctataatgt tcctatatct atctaatttt
     4381 gattgattta gatacttctg tagtagtttt caaaacgttt cttaactctt gtaagtgtat
     4441 tttctttttt taatattttt gagatgtact tattaatgtt tttgactcat tttcagcagt
     4501 aattattgca acagctctat aagtatgtcc ttttgtcaca gaatatgagt ttaaaacaga
     4561 acaagttttt cctccagttt tctttgtaga tgtttttaaa tttacccaac ttccgccttt
     4621 taattcttgt aacttagtta caatagatgt tctagttact ccagattttc caacaacaga
     4681 agcatttaca tttgcttttc cattagaaaa tgataaatta catctaccat ttaatatgta
     4741 aactgaaaaa ggatttattt cttctttacc atcttccata aaaataatat cagatgaatt
     4801 aaactgttca gcaaaactaa caacatttac tgacattatt attatcaacg cacatacaaa
     4861 tattgagatt atatttttta ctctcattaa gtacctcctt ataaaatctt atatattcaa
     4921 tataacaact aactagcact attttagaca aaaatttact taatattttt tgcaatttta
     4981 ataaattctt ttttatcttt cttagttatt ttttttgtag gtagctcttt agataacata
     5041 taaatattat ttttatcatg ccacaataat tgttgagtat ttcctttttc aacatactgt
     5101 gcatactgat tattaaattt tatattctca acaaaagcat cctctgtatc caagactata
     5161 acatccctag aatataacat catttgataa attatatagt tatctaattt atctttatat
     5221 aatattgata gataattttc ttcctcaatt ctctcaactt ctttatatcc agtagaaatg
     5281 tatttaggtt ctatatattt aaatgataca tttgatttat gagggtttat aaaattatag
     5341 tctgtaaatt ctttatatac cttcttcatt attctaatta aattttctgg aaaagcttct
     5401 gtttttatag tagttatagt gactatactt gataaaatta ttataaaaac aatagcaatt
     5461 ttttttaagt ttttataggt gttctttttt acttttttct cattttcttt tataatttta
     5521 tccattttac tttcaaattc tatagaaaat tcatagtcaa tatcttcatc acaaggtaat
     5581 tcatacagta ttttttcttc tatttttgga agctgttcgt ataataattt ttctatgtct
     5641 aatttatctt tattcatttg ataatgcctc catttcgctt aaaatttttt ttaatttttc
     5701 taatgctctt ttatttctct tatacaaatt aacctctttt atattcagta gtttagaaat
     5761 ttctctgata gaaaaaccat gactatattt taagagaatc acctgtaaat ctgtctctga
     5821 cagttttttt aaagcttctc ctaaataact acactcacct aacttttcaa tcgataaatc
     5881 taaagagctg aatttatagt tatcatcatt aatattactt atttcctctt tttttcttct
     5941 attatatata tttattgaaa tcctttttac tataataaca acaaaacctt tagtttttgg
     6001 actattcaca tcatttactt tatcaagatt atcaattatt tttaaaaatg aactatgaac
     6061 agcatcctcc gctaaaacat catcttttaa aatttgatta gctacataaa acattaattt
     6121 cttatattta atatatattt cttcaaattt aattctttct tcttttgaat ctataatagc
     6181 taaatatatt aacataaatt attctccctt aaaaaatatt atccttaaaa aaattatata
     6241 caatttataa aaaattatat ataaatgtct aaaaataaaa acttcatttg ttatatatat
     6301 aaataaaaac ataaaaggat ggtgttttat atgaaatcaa aaagaataaa caaaatgtta
     6361 gcaactacaa tgacaatggc aatgctagca ataccagcag taacagcaac atcatatgca
     6421 atggaatcaa cagggttagg aacagaatat acatataata atgaatctaa acagcaagaa
     6481 gagtgggtta aatcattcga tactgaaaca ggaaaagaaa tattattaaa taaaactact
     6541 aacgaaaaag tttctgaagt atatgaaaat gtaaatggaa aattggttaa attaagtatt
     6601 aatgaagcac tagaaaaatt taatttaaat gaggaaatta tcacaaaatc tcctaagtct
     6661 atttttggag tatcaagtgt taaaaaaatt actggttctg cacgcaaaat atcacaagac
     6721 gtatcaggtg gaaaacaagg tgctactata tctataggaa attcaacaac tgtaactgaa
     6781 agttttactg ttggtggaac tacagaagca ataaaggcag cagtaagagc aaatgcagga
     6841 tttacatgga gcaaatcttt aacaagaagt gtcacaacta cacacaaagt acctgcaggt
     6901 aaaataggat atgtagcatt taaaccatat tataaagaag taagaggtac agtaaatggg
     6961 atacttgcag gaacttctat aactcaaaaa atagtagcaa aaagtcctct taaagcagga
     7021 aaagtttgtg atgggttaga atatcttgca tacaaataat aaataataga aagagttgat
     7081 taaaatggaa aataaaaata tgtacttaag ggttagttta atattagcag tattaggctt
     7141 tataatatta ttttttaata atgacttagc attatcacta aaagcaacat acttagctga
     7201 taaaggcttc gaagacatag ttgaaaatca aatattaaaa aactattcct atatgttttt
     7261 aataataggt ggtgtactat tttccatagg aatctataac ttaaccaaac tgaaacaaat
     7321 taataaaaaa taaataaaat aagaggactt ataaattata agtcctttta ttttacactt
     7381 ataagctact ttcaaccttt catgatttaa cctttattta tacaacaccc aaaactcgac
     7441 ctcaaaaaga gaacaggact tcgtccccct taatacaaag caacattttt tagtactatt
     7501 cgtacttaag attagctatt agattaatcc ttccaaaaga acctcttttt ccgtttcatc
     7561 aatcgcattt aaaagtttat ttattctagc ttcaactaaa tgctctatgc ttaaatcatt
     7621 gtctattagt cctaattctt tttttatgtc ctctatgtct atatgtttct taatagtgta
     7681 aactctaatt acttttttat ctgtcacagg gtttggaagt atatttttat agtatttata
     7741 gggcaaccct gtgacacccc tgtgttgttt gacttcaata acaaaaattg attgtaatat
     7801 tagacttatt ttcttaatag ttagcttttt cttagttgct aaaaacgtct gtaaatcaac
     7861 ggctagagct tctaagtgtt cttttgtata acttgtacca actccaaaat tgaagtttat
     7921 ataattgtaa gtataagtac caatattatc atcatttagc aaagaatttg cgtatttttt
     7981 attaattttt cgataaattg ttgtatttat ctgttcaaaa aatagatttc ttttctcgtc
     8041 tgacattgtc gcaagtttcc aagataggtc aatactataa ctattatcta atacaaaatc
     8101 actaaataag tctatcgttt ttctacaatt attattaatt attaggctat ataaatcatg
     8161 ttccatataa tattttaaac agatattatt atctatcctc atgtcatgat gatattttaa
     8221 taagttactg ctggtcattg acttcttaat ttcactatag ccaactagat actttttatg
     8281 tccttctagt tgttctatag ctgatttctt aaattcctta atagcttcct catgctcttt
     8341 tatttcttcc tctatttttt tagtttcttt ttcagtttct actatctcaa ttttattaaa
     8401 atattcctct aatagcactt tgaagctgtt tattgttctt gtttggtaat agttattgta
     8461 agcattactt tttatgtata acttgtccgt tttatagcac atatcttttt tatcatagta
     8521 tatattacta tcaatgtttt ttatgtttat agggcttata tcaattccta tagctctata
     8581 tggtaagtct ctcttagaag ctttattgta tatatttgtt aggctctcaa cgtcttttat
     8641 gttctcattt atgcaccatt caaccgaata tacattgcta tcctcatttg tcgttttgta
     8701 actattaaaa atatgtacat ttatactttt tgcccctcta gctcttgcaa catactgttt
     8761 tatttttcct acatcctttt ctccaattac aataatatct gttacattag gattatttat
     8821 gtttacacct gccgtgatta cagacgtatt taaaagcacc tcatagcctt ccatatcgga
     8881 gtaactaaca attctatcat atagtttgct agtgtcttta gtatctgaaa taacaactcc
     8941 actcttttta ttaactttct ttgatatata gttaagtgtt gatatactat cttttaaaag
     9001 cattgaatta tttgaattat ttagtatctc tattactttc ccaatattga ttttatcgta
     9061 taacttaact ttgtattccg tttgtatatt ttgtttatat tcaataactt tgttatatat
     9121 ctcaaattct aatttagagg gtgttgcagt tatatctagt ctacctctgc acttattagt
     9181 tatattattt aagctcttta ttgctttgct tctgaacgca tctgtgtaag tctgatgtat
     9241 ttcatcaata actattatgt actcgctcaa atcctctttt agaagctgtt cagtcttatc
     9301 ccacgtcata gccactaact tgtttccctc taaagcgtgt ttagtaggta tcttatcata
     9361 tgcaccagct attttatatt cgactattgc ttgttgcaca ttcgcagagt tggggaatat
     9421 aaagagtgct tttatatcca gctcttttaa tgtatttata attgagtatg tttttccact
     9481 tcctgtagga gctaccatta atacgttctt accttctgta gcatgtttaa ttgactctct
     9541 aatacctttt agattttcac ttatgtattt atctatatta attatcgaat cagctttata
     9601 acttgaatag ttttgttttt ctagcatgga ttttatttta atattgttat ctaaataata
     9661 ctttcttttg tccttatcag tcatacattc aaattcaaaa gctaactcaa tatcattatt
     9721 cctaaaagct tcttgtttct tttcttctat gaagctattt atatcttttt tctttatgta
     9781 aatgtttttg ggaacattat ttattttctt agtgttagtt ttatttttag taaatggcaa
     9841 tggaatatta tatttgttgg ctaagaatag tatagcttca ataggctttt caaagtgttc
     9901 atatgtacga cataactcaa ttatgtctgc accaacaaca cacccatgac tatagcatgt
     9961 atatattctt ttgtttaaat caaattgaaa gtttttatcc ttaccaccat gaactgggca
    10021 agggacattc cctttttgag ttgtatcgac tcctatatct cttaaatata gttcgtctat
    10081 attagcatct tttattatat caattaattg ttttatatct attttcattt gttaaactcc
    10141 ccctatttcc tatttgggag tcaatcaagt tttatcaagc tatgtattta cattctgttg
    10201 taaatcccgt ataattaact tattaaaagg atatttttat cctgtacgtt tgttaattta
    10261 gtttaagtga ttaatctgta gcgattaact cttatacttg attgactcct tttgaaagaa
    10321 tgagtggttg ccgtcaaaca aagactcatt ctttttctat tttttgttat tagtattata
    10381 tcattaataa aagtataatt gacggtttta atatatattt ttaattcctt tttaagcata
    10441 gttttttatt taaggctata aattcagcta ggtcaagccc atctaaaaca cccattcggt
    10501 atgcgtattc cttttcaatt acatttatcc cgaatatatt ttcttcaaaa tcaattaaca
    10561 tttttttatg ttctttttct tctaatatat ccaataatat gtccatagct ctttcagatt
    10621 cttttttgtc atttaaatat ttcttatcat tttccaatag ttcatcctct acattttccc
    10681 tgtatgtgtc aaatccaatt cttaataaat catcttttaa cattactttt aacactcctt
    10741 tttatgcaca cttaaatctt ttgcacccct aaatatggta atatattcta tttattatta
    10801 tgtaattatt tactatgtat tgtcaagcat atatattgtt tttggtacat atttccgata
    10861 ctatataata aaaaaggtgg cgattaaatt gttggatata caaataaata aattattgga
    10921 aaatcaaaat aaaacacgtt attggttagc aaagcaaatt ggaatgactc atcaaaactt
    10981 aactaaacta gccaacaata atacaaattc tattaaattt gattctttag aaaaaatttg
    11041 tatggctcta aaatgttctc ctaacgagct gttcggttgg gagcaagaca aataaaaaaa
    11101 tataaatcct aaagggttta tttataacta ctagaaaact actttagtag ttattttatt
    11161 ttggaggttt aaacatggat agatatacat ttgataactt acctatcaag gagcaaatac
    11221 aatatattaa taataagctc ttagagggca atacattaac taacacatgt aaagatattg
    11281 gtattggtcg tacaactata agaaatagat ttaaacaaca tggatatgaa tttaatccaa
    11341 ctaagaagct atatatatct attattgagg ttgtcgaatt agaaagtagt tcaaaagtag
    11401 ttgcgggcga cactagcaat gacaatactt tagttgatgc aaccaatatt gattttaata
    11461 acattttatc taaattcaat gatatgaata ataaactaaa tgaagtatat agttggtacg
    11521 aatcacaaag tagtaataaa gtagttgggg ctgataaact aaatatagac gattttaaag
    11581 gcaatatagt tgttagaagc tataaggtct atgaggatat tcaaaaagag tttgtagcct
    11641 tctgtgaagc taataagaag tacagggtac aagatattat cagccaagct cttagggatt
    11701 ttatggataa atataagtag ttttacttac tttctgatat tctaaaaact tttcaatgaa
    11761 aatctcattt tttggcttcc tgtgaaaaat atgtactcct tccagtcctc tatctttatt
    11821 tactttatta atccgatagg gggcgtatta tatatatttc tatcgagcat atactcatca
    11881 aaacatagca ttagtttgat tattatattt ctatttattt taagattata tatacaattt
    11941 gccccttaat acattgcaat tactaacttc tttagttttg atattaatat ttaaatcatt
    12001 cctttttaaa ggtgctataa gtgctatata ggcattgcaa cctatatact ttttaggggt
    12061 gcagttgcat atgcaagctc tttatttccc aacacttaac cagttataaa tatttatatc
    12121 ctttattagc caaatatagc attttaaaca actccgacta tctttagtag taacatttta
    12181 catagcttta ttaatgtatt tttgcatctg caaccatgtt tttaagttgc aacaccccac
    12241 ccccacacct tacacaaaac aatacttatc tccgaagcca tatacctacc tcggttcaca
    12301 tatttttctt caacagcgtt attttcagca aggggggcta aacattatac tttttttcag
    12361 tgcattaaat agccttacat gtattggtat aactttattt caaaccctta attgaattgt
    12421 ttcaatctaa attaagtaaa ttttaataag ataaaatata tagtttgtaa ctttatttca
    12481 aaatacag
//
LOCUS       DAMJYR010000111        12414 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.114, whole genome shotgun sequence.
ACCESSION   DAMJYR010000111 DAMJYR010000000
VERSION     DAMJYR010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12414)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12414
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.114"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            <1..321
                     /locus_tag="PC471_002930"
     CDS             <1..321
                     /locus_tag="PC471_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HDF2790040.1"
                     /translation="VEKADCYTGNTIESERFFPGWLYDEEDEFVQAAYKGLKEAGIDS
                     EITQYSFCTNGSHYAGEAGIRTIGFGPSKENLAHTIDEYIELDQLFKGAEGYYGILKS
                     VYKK"
     gene            337..1920
                     /locus_tag="PC471_002931"
     CDS             337..1920
                     /locus_tag="PC471_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacylase"
                     /protein_id="HDF2790041.1"
                     /translation="MRILIKHGLIVDGNKTPAYEGDILIENEKILKISQDLNEEADKV
                     IDAKGRVICPGFIDTHSHSDLVILVNPYNEVKIRQGITTEVLGQDGISMAPLPQEHIS
                     SWRKNLAGLDGESDEIDWKYETTENYLKMMDYNGVGLNETYLVPHGNVRMEAMGLEDR
                     PATKEEIQKMCEITERELKAGAIGLSTGLIYIPCAYSLTEEIIEMCKVVAKYDGVFVV
                     HQRSEADTILTSMEEIIEIGKQSGVKVHFSHFKVCGKANWKYIPQVIELLEKAEKEGI
                     RVSFDQYPYAAGSTMLGVVLPPWAHSGGTDKLIERLSDENERAKMKKDIANGIEGWDN
                     FIEFAGIDQIFVTSVKTEKNKDTIGKNLLEIGKMRGKDPLDATFDLLKEEENAVGMVD
                     FYGLEEHIIGFMKRDEQNVCTDGLLAGKPHPRAYGSFPKILGRYVRELNVLTIEEAVY
                     KMTKKAATSFSIKDRGELKEGYFADIVIFDKDTVSGCDDFINSMQYPTGIDYVIINGN
                     CVVEEGKYNKIKAGKVLKN"
     gene            1948..3276
                     /gene="ssnA"
                     /locus_tag="PC471_002932"
     CDS             1948..3276
                     /gene="ssnA"
                     /locus_tag="PC471_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative aminohydrolase SsnA"
                     /protein_id="HDF2790042.1"
                     /translation="MILVGNGRLISQDSLNPYMEDGCVVINDNLIEDIGTTEEMKAKY
                     PVHEFIDAGKKIIMPGLINSHMHIYSSFARGMAVPGKPSENFLEILNNLWWRLDKQLT
                     LEDTKYSAYATYIECIKNGVTTVYDHHASPNAIEGSLFTISDVAKDLGIRTCLCYEVS
                     DRDGGNTIQQGINENVNFIKYAQKDDTDMIKGMFGLHAAFTLSDDTLNKCASEMAGLD
                     TGYHVHVAEGLADLQYNVDKYGKRVLERFNDFGVLKEKTIAVHCIYISAEEISLLNKT
                     NTFVVHNPESNMGNAVGCSPAIELLKRGVTVGLGTDGYTSDMFESLKVANIIHKHHLK
                     DPRVGFEESQTMLFDNNRKIAKAYYKNDLGVLKKGAYADVIVVDYIPHTPLTENTIGG
                     HTIFGMSGRSVDTTIINGKVLMKDRKLINIDEESILAKSRELSQNLWNRI"
     gene            3475..4827
                     /locus_tag="PC471_002933"
     CDS             3475..4827
                     /locus_tag="PC471_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HDF2790043.1"
                     /translation="MSRDKSPKIAPVDERLPVSKSWVFSLQHVLAMCAGAVAVPMMIG
                     SAARLDHASIVFLINACLFMAGIGTLIQSVGIGNIVGAKIPVIEGTSFAAVAGMTAIA
                     SSYSDSQVAIQTVFGSVIVAGLFCILLAPVFEKLIRFFPSVVTGTVVMVIGISLFPVG
                     IKWITDNKLEAVEPNSLALALAVLAITLLSFKFLKGIWNSTAILVGIVLGTLLSMAVG
                     MADFSAVNGVGWLNLNVPLKFGMPRFEPSAILSLCLIMLVLMTESVGNMIAIHEMVDK
                     EVTGKNIKRGLFGDGISTALSGIFNSFPITPFGQNTGIVGLTGIKSRFIAVYAGVILL
                     VLGFLPKFAAIIGAIPKPVLGGVGFAMFGMVMVGGVKTLSKVKFDGTKNGVIVAVSVG
                     LSMIPLTNPDFYHNFPTWVQTIFHSGITTGSLSAVLLNIFFNELGSKKEKNNKVEEAK
                     "
     gene            5019..6128
                     /locus_tag="PC471_002934"
     CDS             5019..6128
                     /locus_tag="PC471_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HDF2790044.1"
                     /translation="MANLKVNLYGMEFLNPIMTAAGPGAKDGALCQEAAKGGAGAICT
                     KTISVLPADVPRPCMAKTNSGFLNTELWSELPKEQWIEEEYKKVKETGLPVIVSMGYS
                     AEQIAEVAPLVKPYADAVELSTHYVGTDVAPIVNALKAAKAVLDVPVFMKMSPHTDIQ
                     TIAKAVEEAGADGLVMINSFGPCMAIDVNTGYPIMGSKAGYGWLSGAPIRPLAIRCIY
                     EASQVVNIPIIGVGGITCGKDVAEMMMAGASAVQVCTEAILKGPSIYGKIAKELNEFL
                     DENGYADVNEIKGLTHKKMAARGFRTHSVAPVVDDNKCIKCKQCVTSCVYEALEVTDK
                     LNIDADKCFGCGLCVTRCPKGALSICYNQVEAMNE"
     gene            6411..7802
                     /gene="hydA"
                     /locus_tag="PC471_002935"
     CDS             6411..7802
                     /gene="hydA"
                     /locus_tag="PC471_002935"
                     /EC_number="3.5.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropyrimidinase"
                     /protein_id="HDF2790045.1"
                     /translation="MGIVLKGGKVVTSEKTYCADVRIEDEKIVDIGLDISKENDEIVD
                     VSDSYVIPGGIDTHTHFDLDTGVTKTADNFETGTKAAIVGGTTTILDFATQAKGCSLK
                     DGLEEWHEKAKDKSYSDYGFHMAITDWNDSVCNEMEDMVKEGVSSFKLYMAYKGSLQV
                     DDGVIFEALRKAEEIGGIIGFHCENGDIICELVDKAKSENNLSPKYHQLTRPAIMEAE
                     ATSRLMKIAEVANSKVYVVHLSCKESLEEVLKAKQRGVDVIVETCPQYLLLNDELYDL
                     DGFESAKYVMSPPLRNKENNEILWNAILDNQIDTIGTDHCSFNYKGQKELGINDFSKI
                     PNGSPGVEHRMSLLYTYGVCKNKISINKMVEVTSTNAAKLFGMYPNKGTIEIGSDADI
                     LVLNPNKKSKIEAKKQLQNVDYTPYEGYEVDCQIEHVYLRGKEVVRDGELICNTPSGR
                     YINRRLTKGAREW"
     gene            7796..10357
                     /gene="xdh"
                     /locus_tag="PC471_002936"
     CDS             7796..10357
                     /gene="xdh"
                     /locus_tag="PC471_002936"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium-dependent xanthine dehydrogenase"
                     /protein_id="HDF2790046.1"
                     /translation="MVEFILNGEKVVAKEGKKLLDFLREDMNITSVKNGCSEGACGTC
                     MVIIDGKATKACVFKTDKLAGKNITTIEGLSERERDVFAYSFASQGAVQCGFCIPGMV
                     ISSKALIDVNNNPTEDDIKKALKGNICRCTGYVKIIKAVDLAAKIFRENIDVPKVNCK
                     GLVGENLPRVDAVGKALGYSEYVDDMRIEGMIYGSAVRAKYPRALVKKIDIEEAKAYP
                     GVVAVLTAKDIPGTIKVGHLKKDWDTLIPEGEITRYLGDGIVLVAAETREALEEAKKL
                     VKIDYEELTPLSTTADALREDAPKIHETGNILVKEHLVRGNAKEKIENSKYVVTKRYT
                     TPATEHAFLEPECAVAGPYEDDGVIIYSTDQGVFATQHECADMLGLPYEKVRVINKMV
                     GGGFGGKEDMSVQHHAALLAYHTKRFVKVQLTRKESIIVHPKRHATEMEFTTACDENG
                     YLTGMKATIISDTGAYASLGGPVLQRLCTHAAGPYNYQDIDITGTAVYTNNPPGGAFR
                     GFGVTQSCFATECNLNLLAEMVGISPWEIRYKNAIRPGQVLPNGQFADEGTAMAETLE
                     AVKEEYEKHPRAGIACAMKNSGVGVGIPDVGRCRLVIKDGKVHIRTSASCIGQGLGTV
                     MTQVVCETANLLPENIVHEVPDTHLTPDSGNTTASRQTVFTGEATKVASNKLKEALDS
                     GKTLLDLEGEEFYGEYESITDKMGCDKENPVSHVAYGYATQVVILDENGKLEKVVAAH
                     DVGRAINPISLEGQIEGGVVMGLGYALTEDYPVVNSIPTAKFGTLGLFKSTDIPEIQA
                     LIVEKNKDLLAYGAKGVGEICTVPTAPAVQNAYYVFDKEFRTELPLQNTPYSKKK"
     gene            10509..11855
                     /gene="rpoN"
                     /locus_tag="PC471_002937"
     CDS             10509..11855
                     /gene="rpoN"
                     /locus_tag="PC471_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HDF2790047.1"
                     /translation="MNFNNSLELTQSQKLIMTTQLKQSLSILNMSKLELEEEIKREAE
                     NNPLVEVEKSGEINWEEYIKDMDRSRRLDRTEISYNPDNEVNLENLVKYSSNLYEDLK
                     FQISLYKLTDKELEVCEYIIDSLDEDGYLRTEEKVIVDTLKIDEELFEKCLISVQQLE
                     PSGVGARSLSECLMIQMASLGIYNEILEEIVTKDLNLIANNKYKEISKKYNMSLQKCV
                     ELINIIKTLDPKPGRTCSVEKSVYIQPDVTVEKIDDEFIVYLNEKDSYQIRINSYYKD
                     ILKNSQSDESAKEFIKERLNSATGLMKNIESRKTTVLKIAEEIVKAQDEFLRKGTKYI
                     KPLKMKDIAEKLEFHESTISRGVNGKYMLTPFGVYEFRYFFSSAIETENNEMASSTSI
                     KKIIKETIKDENKKKPLSDDHISKLLKEKGINVARRTVAKYREELGIPSSSKRKEY"
BASE COUNT         4966 a         1428 c         2511 g         3509 t
ORIGIN      
        1 gttgaaaaag ctgattgcta tacaggaaat actatagaaa gtgaaagatt cttcccaggt
       61 tggctatatg atgaagaaga tgaatttgtt caagcagcgt ataagggatt aaaagaagct
      121 ggaatagatt cagaaataac tcaatactca ttctgtacga atggaagtca ctacgcaggt
      181 gaggctggaa taagaactat aggatttggt ccttctaagg aaaatcttgc tcatacaata
      241 gatgagtata tagaattaga ccaattattc aaaggtgcag aaggatatta tggtatatta
      301 aaatcagttt ataaaaaata attaggagat ggttttgtga gaattttaat aaaacatggg
      361 cttatagtag atggaaataa aacaccagca tatgaaggag atattttaat agaaaacgag
      421 aaaatattaa aaatatcaca agatttaaac gaagaagctg acaaagttat agatgcaaaa
      481 ggtagagtta tatgtccagg tttcatagat actcatagcc attcagacct agttatttta
      541 gttaatccat ataatgaggt aaagattaga cagggaatta caacagaagt gttgggccaa
      601 gatggtatat ctatggcccc tctacctcaa gagcatatat cttcttggag aaaaaattta
      661 gcaggtctag atggtgaaag tgatgaaatt gattggaaat atgaaacaac agagaattat
      721 ttaaaaatga tggattacaa tggggtaggt ttaaatgaaa cttatttagt acctcatgga
      781 aacgttagaa tggaagctat gggacttgaa gatagacctg ctactaaaga agaaattcaa
      841 aaaatgtgtg aaatcacaga gagagaatta aaagcaggag ctataggatt gtctactgga
      901 cttatatata taccttgtgc atactcattg actgaagaaa ttatagagat gtgtaaggta
      961 gtagcaaaat atgatggagt atttgtagtt caccaaagaa gtgaagcaga tacaatttta
     1021 acttcaatgg aagaaatcat agaaattgga aaacaatcag gagtaaaagt tcatttttca
     1081 cattttaagg tttgtggtaa agcaaactgg aagtatatac cacaagttat agagctttta
     1141 gaaaaagcag aaaaggaagg aataagagta tcttttgacc aatatccata tgcagcagga
     1201 agtacaatgc ttggagtagt tttacctcct tgggcacatt ctggaggaac tgataagtta
     1261 atagaaagat taagtgatga aaacgaaaga gcaaaaatga aaaaagatat agcaaatgga
     1321 atagaaggct gggataattt tatagaattt gctggaatag accaaatatt tgtaacaagc
     1381 gtaaaaacag aaaagaataa agatacaatt ggtaagaact tattagaaat tggaaaaatg
     1441 agaggaaaag acccattaga tgcaacgttt gatttattaa aagaagaaga aaatgcagtt
     1501 gggatggtag atttctatgg attagaagaa catataatag gattcatgaa aagagacgag
     1561 caaaatgtgt gtacagatgg tcttttagca ggtaaaccac atccaagagc ttatggttct
     1621 ttccctaaaa tactaggaag atatgttaga gagttgaatg tgttaacaat cgaagaagct
     1681 gtttataaaa tgactaaaaa ggcagctaca agctttagta taaaagatag aggcgaatta
     1741 aaagaaggat attttgcaga tatagttata tttgacaagg atacagttag tggttgtgat
     1801 gattttatca actctatgca gtaccctaca ggtatagatt acgtaattat aaatggaaat
     1861 tgtgttgttg aagaaggaaa atacaataaa attaaagcag gaaaagtact taaaaattaa
     1921 atcttagctt taaggaggaa taaatttatg attttagtgg ggaatgggag gctaataagc
     1981 caagattctt taaatcccta tatggaagat ggatgtgtag taataaatga taatctaatt
     2041 gaagatatag gtactacaga agaaatgaaa gctaaatatc cagtacatga atttatagat
     2101 gcaggcaaga aaataatcat gccgggtcta ataaattcac atatgcatat atacagctca
     2161 tttgctagag gaatggcagt tccaggaaaa ccttctgaga actttttaga gatactaaac
     2221 aatttatggt ggagacttga caaacagctt acactggaag atacaaaata cagtgcatat
     2281 gcaacgtata tagaatgtat aaaaaatggt gtaacaacag tttatgacca tcatgcaagt
     2341 ccaaatgcaa ttgaaggcag tttgtttaca atatcagatg ttgcaaagga tttgggaatt
     2401 agaacgtgtc tttgttatga agtgtctgat agagatggtg gaaatacaat ccaacaaggt
     2461 atcaatgaaa acgtaaactt tataaaatac gctcaaaaag atgatacaga catgataaaa
     2521 ggaatgtttg ggcttcatgc tgcatttaca ctatctgatg atacattaaa taaatgtgct
     2581 tcagagatgg ctggtcttga tacaggttac catgtacatg ttgctgaagg actagcagac
     2641 ttacaatata atgtagataa atatggaaaa agggttttag aaagattcaa tgattttgga
     2701 gtactaaaag aaaaaacaat agcagttcat tgtatatata taagtgcaga agaaatcagt
     2761 cttttaaata aaacaaatac atttgtagtt cataatccag aatctaatat gggaaatgca
     2821 gttggatgtt ctcctgcaat agagttatta aaaaggggag ttacagtagg attaggaact
     2881 gatggatata ctagcgatat gtttgaatca ttaaaagttg ccaatataat tcacaaacat
     2941 cacttaaaag acccaagagt tggatttgaa gaatcacaaa caatgttatt tgataacaat
     3001 agaaaaatag ctaaagctta ttataaaaat gacctaggag tacttaaaaa aggtgcttat
     3061 gcagatgtta tagtagttga ttatatacca catactccat tgacagaaaa tacaataggg
     3121 ggacacacta tttttggaat gagtggaaga agcgtagata caactattat aaatggaaag
     3181 gtacttatga aagatagaaa gttaataaac atagatgaag aaagtattct agcaaaaagt
     3241 agagaattaa gtcaaaatct atggaataga atttaaataa ttgattatgc aaaattctat
     3301 gaagcaggaa ccctgtgact ttagtcatgg gaggttcaga ttaatataca agtaagaaac
     3361 aagaatttag atgttaaaaa cagagcttaa acaaaaatag gaatttaaat gaagtatata
     3421 aataaaaaat atacggaata gaatattaaa ttaaatatag aagggagacc caaaatgtct
     3481 cgagataaaa gccctaaaat agctccagta gatgaaaggt tacctgtatc caaatcttgg
     3541 gtattttcac tacagcacgt tttagcaatg tgtgcaggag cagtagcagt tcctatgatg
     3601 ataggaagtg ctgcacgact agatcatgct agtattgttt ttttaataaa tgcttgcttg
     3661 tttatggcag gtataggaac actcatacaa agtgttggta taggaaatat tgtaggtgct
     3721 aaaataccag taatagaagg aactagtttt gccgctgtgg caggtatgac agcaatagcc
     3781 tcatcatata gtgattcaca agttgcaata cagacagtat ttggctctgt aatagtagct
     3841 ggattgtttt gtatattact tgctccagta tttgaaaaat taataagatt tttccctagt
     3901 gtagttactg gaacagttgt tatggtaata ggtatttcac tatttccagt tgggataaaa
     3961 tggataactg acaacaaatt ggaagcggtt gaaccaaact cattagcatt agctttagca
     4021 gtattggcaa taactttact ttcatttaaa ttcttaaaag gaatttggaa tagcacagct
     4081 atattggtag gtatagtact tggtacacta ctttccatgg cagttggaat ggcagatttt
     4141 agtgcagtaa atggagttgg ttggcttaac ttaaatgtac ctttaaaatt tggaatgcct
     4201 agatttgaac catctgcaat actatcattg tgtttgatta tgttggtact tatgacggaa
     4261 tcagttggta atatgatagc tattcatgaa atggtagaca aagaagtaac aggtaaaaat
     4321 attaaaagag gtctatttgg agatggaata tctacagcat tatctggaat attcaattca
     4381 tttccaataa ctccatttgg tcaaaataca ggaatagtag gtcttacagg tataaaatca
     4441 agatttatag cagtttacgc aggggttata ttgctagtac ttggattctt accaaaattt
     4501 gcagcaataa taggagcaat acctaagcct gttcttggag gcgtaggatt tgcaatgttt
     4561 ggaatggtaa tggttggagg agtaaaaact ttaagcaaag tgaaatttga tggaactaaa
     4621 aatggggtta tagtagcagt tagtgttggt ttgtctatga taccactaac aaatcctgat
     4681 ttctatcata atttcccaac ttgggtacaa acaatattcc atagtggaat aacaacaggt
     4741 agtttatcag cagtgctttt aaacatattc ttcaatgaac taggttctaa aaaagaaaaa
     4801 aataataaag ttgaagaagc aaaataaaaa taaaacaagt aaaaatctat attgacttaa
     4861 agtcattaag tattaataaa aataattcag agatattaat aattcaacaa aactatctta
     4921 tattttaaat aatataaaaa agtaaataaa taacaaatac aaataaaaga atacaatcta
     4981 aatattaaaa atatttaatt taagggagag ataataatat ggctaattta aaagttaatt
     5041 tatatggaat ggaattttta aatccaatta tgacagcggc aggacctgga gcaaaagatg
     5101 gtgctctctg tcaagaggca gcaaaaggtg gagcaggagc tatatgtaca aaaactatat
     5161 ctgtattacc agctgatgta ccaagaccat gtatggcaaa aactaatagt ggttttttaa
     5221 acacagaatt atggtcagag cttccaaaag agcaatggat agaggaagaa tacaaaaagg
     5281 taaaagaaac agggcttcca gtaatagtaa gtatgggata ttcagcagag caaatagctg
     5341 aggttgctcc tttagtaaag ccttatgcag atgcagttga attatctact cattatgtag
     5401 gtacagacgt tgcaccaata gtaaatgctt taaaagctgc aaaagctgtt ttagatgtac
     5461 cagtatttat gaaaatgagt cctcatactg acattcaaac tattgcaaaa gctgttgaag
     5521 aagcaggtgc agatggactt gtaatgataa actcatttgg accatgtatg gcaatagatg
     5581 taaatacagg ataccctata atgggaagta aagctggata cggatggtta tcaggtgcac
     5641 caataagacc tctagcaata agatgtatat atgaagcttc tcaagttgta aatataccaa
     5701 taatcggtgt tggtggaatc acttgtggaa aagacgtggc tgaaatgatg atggctggag
     5761 caagtgctgt tcaagtatgt acagaagcaa tccttaaagg gccttctatc tatggaaaaa
     5821 ttgctaaaga attaaatgaa tttttagatg agaatggata tgctgatgtt aatgaaataa
     5881 aaggactaac tcataagaaa atggcagcta gagggtttag aactcattca gtagctccag
     5941 tagtggatga taacaagtgt atcaaatgta agcaatgtgt aacaagctgt gtatacgaag
     6001 ctttagaagt tacagataag ttgaacatag atgcagacaa gtgttttgga tgtggattat
     6061 gtgtaactag atgtccaaaa ggagctttaa gcatctgcta caatcaagta gaagctatga
     6121 atgagtaata attatataga attttggtaa ttaacttaga gtaataggta ttaggataat
     6181 aaaatatatc agataaacaa acacacatta tgcattaata atacagtcta aagttaataa
     6241 ttttattata ttgatttaac taaatattag tttagctgaa tattaattta attaggtatt
     6301 ggtttaatct taatatagat ttactgcgta taagtgaact aactagatta ctttaagtta
     6361 attttttcat ataaaataaa ttgatacatt acaagtaagg tggagtctat atgggaattg
     6421 ttttaaaagg tgggaaagta gtaacaagtg aaaaaactta ctgtgcagat gtaagaatag
     6481 aagatgaaaa aatagtcgat ataggtcttg atatatcaaa agaaaatgat gaaatagttg
     6541 atgttagtga ttcttatgtt ataccaggag gtatagatac acacacacac tttgacttag
     6601 atacaggcgt tactaagaca gcagataact ttgagacagg aacaaaagct gctatagtag
     6661 gaggaactac tactatattg gattttgcta cacaagctaa aggatgttct ttgaaagacg
     6721 gattagaaga atggcatgaa aaagctaagg ataaatccta ttcagattat ggctttcata
     6781 tggctataac agattggaat gactcggttt gtaatgaaat ggaggatatg gtgaaagagg
     6841 gagtatcttc atttaaactg tatatggcgt ataaaggaag ccttcaagtt gatgatggtg
     6901 taatctttga agcattgaga aaagcagaag agataggagg gattattggt ttccattgtg
     6961 aaaatgggga cataatatgt gaacttgtgg ataaagcaaa gtcagaaaat aatttatctc
     7021 caaaatatca tcaattaact agaccagcta taatggaggc agaagctaca tcaagactta
     7081 tgaagatagc tgaagttgca aactctaaag tatatgtagt tcacttaagc tgtaaagaat
     7141 ccttagagga ggttttaaaa gctaagcaaa gaggtgtgga tgtaatagtt gaaacttgtc
     7201 ctcaatacct tctattaaat gatgaattat atgatttaga tggatttgaa agtgctaagt
     7261 atgtaatgtc accaccttta agaaataaag aaaataatga aatattatgg aatgctattt
     7321 tagataatca aatagacact ataggaacag accattgttc ttttaattat aagggacaga
     7381 aggaacttgg gataaacgat ttcagtaaga taccaaatgg ttctccaggt gtagaacata
     7441 ggatgagtct tttatacaca tatggagtct gtaaaaataa aatatctatc aacaagatgg
     7501 tagaagtaac atcaactaat gcagcaaagt tgtttggaat gtatcctaat aaaggaacta
     7561 tagaaatagg cagtgatgca gatatattag tcttaaatcc aaacaaaaaa tccaaaatag
     7621 aagcaaaaaa acaattacaa aatgtagatt atacacctta tgaagggtat gaagtggatt
     7681 gtcaaataga acatgtttat ttaagaggaa aagaagtagt tagagatgga gaattaattt
     7741 gtaatactcc aagtgggaga tatataaata ggcgtctaac aaagggggct agagaatggt
     7801 agaatttata ttaaatggag agaaagttgt tgcaaaagaa ggcaaaaaac ttttagactt
     7861 ccttagagaa gatatgaata taacttcagt taaaaatgga tgttcagaag gtgcttgtgg
     7921 aacatgtatg gttataatag atggtaaagc tactaaagca tgtgtattta aaactgataa
     7981 attagctggg aaaaacataa caactataga gggattatca gagagagaaa gagatgtttt
     8041 tgcatattct tttgctagtc aaggagctgt acaatgtgga ttttgtattc caggtatggt
     8101 aattagttca aaagcactta tagacgttaa taataatcct actgaagatg atataaagaa
     8161 agctttaaaa ggtaatatat gtagatgtac agggtatgta aaaataatta aagctgtcga
     8221 tttagcggca aagattttta gagaaaatat agatgtgcca aaagtaaact gtaagggtct
     8281 agtaggtgaa aatcttccta gagttgatgc agttggcaaa gcacttggat attctgaata
     8341 tgtagatgat atgagaattg agggtatgat ttatggttca gcagttagag ctaagtatcc
     8401 tagagcttta gttaaaaaaa tagatataga agaagcaaaa gcttatccag gagttgttgc
     8461 agttctaaca gctaaagata ttccaggaac tataaaggta gggcacttaa agaaggattg
     8521 ggatacttta ataccagaag gtgaaataac tagatactta ggagatggaa tagttttagt
     8581 agcagcagaa actagagaag cgttagaaga ggccaaaaag ctagttaaga tagattatga
     8641 ggaattaaca ccattaagta ctacagcaga tgcacttaga gaagatgctc caaagataca
     8701 tgagacaggg aatatacttg ttaaggaaca tttagtaaga ggaaacgcta aagaaaagat
     8761 agaaaattca aaatatgttg taactaaaag atatactaca cctgctacag aacacgcatt
     8821 tttagagcct gaatgtgcag tagctggtcc ttatgaagat gatggagtaa taatctattc
     8881 gacagaccaa ggggtgtttg ccactcaaca tgaatgtgca gatatgttag ggttaccata
     8941 tgagaaggta agagttataa acaaaatggt aggaggaggg tttggtggaa aagaagacat
     9001 gagtgttcaa caccatgcag ccttacttgc ataccataca aagagatttg taaaagtaca
     9061 gttaacaaga aaagaaagta ttattgttca tccaaaacga catgccacag aaatggaatt
     9121 tacgacagca tgtgatgaaa atggatactt aacgggtatg aaggcaacga taatatctga
     9181 cacaggtgca tatgcctctc ttggaggacc agtgctacaa agactgtgta ctcatgcagc
     9241 aggaccatac aattatcaag atattgatat aacaggaaca gcagtatata ctaataaccc
     9301 tcctggagga gcatttagag gatttggagt tacacagagt tgttttgcaa ctgaatgtaa
     9361 tctaaattta cttgctgaaa tggttggaat atctccttgg gagataagat ataaaaatgc
     9421 aataagacct gggcaagttt taccaaatgg tcaatttgca gatgaaggaa cagcaatggc
     9481 agagacacta gaagctgtaa aagaagagta tgaaaagcat ccacgtgcag gtatagcttg
     9541 tgctatgaaa aatagtgggg ttggagttgg tatacctgat gttggaagat gtagattggt
     9601 gataaaagat ggcaaagttc acataagaac aagtgcttct tgtataggtc aagggcttgg
     9661 tactgttatg actcaagtag tttgtgaaac tgctaaccta cttcctgaaa atatagttca
     9721 tgaagttcca gatactcatt taacaccaga ctctggtaat actacggctt caagacagac
     9781 agtatttacg ggagaagcta ctaaggttgc atcaaataag ttaaaagaag ctttagactc
     9841 tggcaaaact ttacttgatt tagagggaga agagttttat ggagaatatg aaagtattac
     9901 agataaaatg ggttgtgaca aagaaaatcc agtaagtcat gttgcttatg gatatgcaac
     9961 acaagttgta attctggatg aaaatggaaa actagaaaaa gtagtagcag cacatgatgt
    10021 tggaagagct ataaatccaa tcagtttaga aggtcaaata gaaggtggag tagttatggg
    10081 tcttggatat gctctaaccg aagattaccc agtagttaac tctattccta cagctaagtt
    10141 tggtacatta ggtttattta aatctacaga tataccagaa atacaagctt taatagttga
    10201 aaagaacaaa gatttattgg cttatggggc aaaaggtgta ggagaaattt gtacagttcc
    10261 tactgcacca gcagtacaaa atgcctatta tgtatttgat aaagaattta gaactgagtt
    10321 accactacaa aatactccat atagtaaaaa aaagtaacaa aaaaaagaaa tattaaataa
    10381 aaaaatagat gaaattgtaa ataaaaaaaa cccttttgca taaggtatat tttaaatgat
    10441 agaatataca tatacaggag ggtttttctt ttataaaata agtttaataa aattgagagg
    10501 tgagcagaat gaattttaat aatagtctag aattaactca gtctcagaag ctaataatga
    10561 ctacacaact taagcaatca ttatcaatat taaatatgag taagttagag ttagaagaag
    10621 agataaaaag agaagcagag aataacccat tagttgaagt cgagaaaagt ggggagataa
    10681 attgggaaga gtatataaaa gacatggata ggtcaagacg tttagataga acagagataa
    10741 gctataatcc tgataatgaa gttaatctag aaaatttagt taaatattcg tcaaatttat
    10801 atgaagattt aaaatttcag ataagcctgt ataaacttac agacaaagaa ttggaagtct
    10861 gtgagtatat aattgatagt ttagatgagg atggatattt aagaactgag gaaaaagtaa
    10921 ttgttgatac cttaaagata gatgaagaac tttttgaaaa atgtttaatt agtgttcagc
    10981 agttggaacc tagtggagta ggggcaagaa gtctttcaga atgtctgatg atacaaatgg
    11041 ctagtttagg aatatataat gaaattttag aggaaatcgt tacaaaagac ttaaatttaa
    11101 ttgcaaataa taaatacaaa gagataagta aaaagtacaa tatgtcatta caaaaatgtg
    11161 ttgaactaat aaatataatt aagacattag accccaaacc aggaagaact tgttcagtag
    11221 agaaaagtgt atacattcaa cctgatgtaa cagtagaaaa aattgatgat gaatttattg
    11281 tatatttaaa tgagaaagat tcttatcaaa taagaatcaa tagttattat aaagacatat
    11341 taaaaaattc acagtctgat gaaagtgcaa aagaatttat aaaagaaaga ctaaattcag
    11401 caacaggact tatgaaaaat atagagagta ggaaaactac agtacttaag attgcagaag
    11461 agatagtaaa agctcaagat gaatttttga gaaaagggac aaaatatata aaacctttga
    11521 aaatgaaaga catagcagaa aaattagaat ttcatgaatc tactataagt agaggtgtaa
    11581 atggtaaata tatgctaaca ccttttggcg tatatgaatt taggtacttt ttcagtagcg
    11641 ccattgagac tgagaataat gaaatggcat ctagtacaag cataaaaaaa attataaagg
    11701 aaacaataaa agacgaaaat aaaaagaaac ctttaagtga tgaccatata tctaagttat
    11761 taaaagaaaa aggaatcaat gttgcaagaa ggacagttgc caaatatcgt gaagaattag
    11821 gaatcccatc ttcaagtaag agaaaagaat actaataagt aaaagacaaa tatcagtatg
    11881 gtaaggggaa aaggctaatt aagagatgaa tgtagattgg gctaaaaatt ttataaagta
    11941 aaataatata atatgaaaaa gtattaaaat gctataaggt attaaaaaac tggggaacat
    12001 taaaacatta agatagcaaa gagcataaaa aactaaagaa gattaaaaca ttaaagaaca
    12061 ttagagcttt gaggaatatt agaactttaa agaatattaa aatttataaa atataaaaat
    12121 actaaaaaga attgaaaaac tggggaacat taaaatattg taaaatgcta aaaaactaga
    12181 aaacataatt aaataagtgc ttataaagaa tgattaaatt aaataagtgt ttgtaaagaa
    12241 taattagata ttagaaaaag aagaatacct agataaataa actattatta aatttaataa
    12301 tcacaaaagg aaaacacgta gggtataaat aaaaatctta actagataaa agtaaactta
    12361 gttgtagata aaaataacta gaaataaaat tatttatagt aataaatatt agaa
//
LOCUS       DAMJYR010000112        12131 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.115, whole genome shotgun sequence.
ACCESSION   DAMJYR010000112 DAMJYR010000000
VERSION     DAMJYR010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12131)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12131)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12131
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.115"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            361..816
                     /locus_tag="PC471_002938"
     CDS             361..816
                     /locus_tag="PC471_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HDF2790048.1"
                     /translation="MDEIDLKIINSLKKNSRASTSEISRQINLSVPAVSERIRKIEQS
                     NLIQKYTIQINREKTKYKLLAFIFINTDLSKSMKEFKKTILEYDTVLECHHISGEYDY
                     LIKVLAEDTSNLEEFISVTLKEIGGIQKANTTIVLSTIKEEFNTIKDIF"
     gene            865..1449
                     /locus_tag="PC471_002939"
     CDS             865..1449
                     /locus_tag="PC471_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HDF2790049.1"
                     /translation="MKVLVFLAKGFETMEFSVFVDVMGWARNDYGHDIDVVTCGFKKQ
                     VMSTFNIQVLVDKTIEEVCVDDYDALAIPGGFEEFGFYDEAYDSSFLNLIREFNSKEK
                     IIASICVAALPVGKSGVLKNRKATTYHLKNGKRQRQLSEFDVNVVNEPIVVDKNIITS
                     YCPETAPHVAFKLLEMLTSKEQMDEVKLAMGFKL"
     gene            complement(1454..1777)
                     /locus_tag="PC471_002940"
     CDS             complement(1454..1777)
                     /locus_tag="PC471_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2790050.1"
                     /translation="MKKCLDNYSCPIEATLALIGGKYKTLILWHLKDTILRFNELKKL
                     IPKATPKMLTQQLRELESDGLIIRVVYPVVPPKVEYSLSDFGKSIIPILDSMCDWGSD
                     YLENL"
     gene            1929..2447
                     /locus_tag="PC471_002941"
     CDS             1929..2447
                     /locus_tag="PC471_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HDF2790051.1"
                     /translation="MNFVELAKKRYSCRNYQDRKVEKEKLEKVLDVARIAPTGGNRQP
                     QRLIVIQEKEGINKLSKAANIYDAPLAILVCGDKDKVWTRPFDGKQLTDIDTSIVTDH
                     MMLQATELGLASVWVCYFNPDIIREEFSLPDNLEPINILLMGYESKIPESPERHEKTR
                     VPLSEIVSYETL"
     gene            complement(2623..3291)
                     /locus_tag="PC471_002942"
     CDS             complement(2623..3291)
                     /locus_tag="PC471_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2790052.1"
                     /translation="MLEISHKVKEIYPNIKFGVMVINITHSKPNKENFLVLKNSTIKN
                     IIEQHPEYNRKEKIKTEPVSSYIKYYKKFKKTYPVLLQLESILLKSKGIPDVGVTIES
                     MFLAELKNLLLTAGHDLDKMELPLKIELANGSEHFYGIGGKEHILTKDDLFLSDNIGI
                     LSSILNGPDNRTAITKDTKNIMYFVYGPDKISEKQIQDHLNDIKHYISSAFPDLKVNS
                     IDIF"
     gene            3406..4821
                     /locus_tag="PC471_002943"
     CDS             3406..4821
                     /locus_tag="PC471_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HDF2790053.1"
                     /translation="MWITINRNSNISLSRQIYCEIKDMILDGSLKFEQKLPSSRTLSK
                     ELSVSRNTVLDAYNQLIAEGYLETRNGYGTIVAKGISGHKVDFKLNDTFRDEKDLHTN
                     KQKNYIDFRSGVPELKLFPRKELSKLYKQILYDLSDSDFRYGSTAGVWVLRVEISKYL
                     FRTRGIRCNPKNIMIVSGSTQGLSLISQLLYKSNKKVAVEDPIHKGLLKVISSIGYSV
                     VGIKVDDKGMNTNLIKSIKGISFVYTTPSHQYPLGGILPIQRRLELIKCATENDYYII
                     EDDYDSEFRYEGQPISSLYELSPNKVIYIGSFSKILSPAIRVGFMLLPDNILESYKTL
                     KMYSDVHTETILQHVLAEFIGNGGLEKYIFRMKKLYNRKRKYLIKVLNTEFKNEFKIK
                     GEAAGLHIVAHFYNVKFTESLIHKIFEAGIKVYPIQQYAIENILEYDYEIILGYAHLS
                     FDEIEKGVKILSKIIHMNQGT"
     gene            5044..5502
                     /locus_tag="PC471_002944"
     CDS             5044..5502
                     /locus_tag="PC471_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2790054.1"
                     /translation="MEIIDYIEENLTENLNLDKIAYIANYSKYHLHRMFTNVVGCTIH
                     QYIQRRRLTEAARQLIFTDKYIIDIAITVGYSTHQSFTLAFRNLYKESPQVYRNKDEF
                     YPIQQRFVLNLNHQIQSEKCISYANMNYKQRRDEINSYLNFNINCEVKVA"
     gene            5499..6083
                     /gene="clpP"
                     /locus_tag="PC471_002945"
     CDS             5499..6083
                     /gene="clpP"
                     /locus_tag="PC471_002945"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HDF2790055.1"
                     /translation="MSLVPHVIEQTGQGERSYDIYSKLLKDRIIFIGEEINDAIASLV
                     VAQLLFLESEDPDSDIIIYINSPGGSATSGFAIYDTMNYVRCDVSTICIGMAASMSAF
                     LLAGGTHGKRCALPNSEIMIHQPMGGAKGQATDVKIAVDNILKIKERLDKILSENTGK
                     SIEDIRRDTDRDNFMTALEAKEYGLIDYIMNRNE"
     gene            6395..7471
                     /locus_tag="PC471_002946"
     CDS             6395..7471
                     /locus_tag="PC471_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HDF2790056.1"
                     /translation="MITISLCMIVKNEEEVIGRCLECVKDIVDEIIIVDTGSTDSTKE
                     IVSKYTDMVYDFEWIDDFSAARNFSFSKASKDYTMWLDADDIILEADREKLLKIKESL
                     DTSIDIVMAKYNVSFDENGNPTLSYYRERLFKRSSNHKWVDPIHEVVPLFGEVFYSDI
                     AISHKKLHRQDPLRNLRIFEKMISEGKTLEPRHQFYYSRELYYNARYKEAIEGFTKFL
                     DSSRGWIEDCISACRDLATCYYLINDEKSALYSLFRSFEFDEPRAEICCDIGKHMFDR
                     QKYKEAIFWYKVALTRDKNDTDGGFKSDDCYGYIPSIQLSVCYDRLGESDKAIYYHEK
                     TKEIKPNDSAVLHNENYFSKFKNS"
     gene            7538..9613
                     /gene="bclA3"
                     /locus_tag="PC471_002947"
     CDS             7538..9613
                     /gene="bclA3"
                     /locus_tag="PC471_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-like exosporium glycoprotein BclA3"
                     /protein_id="HDF2790057.1"
                     /translation="MLLIMSRNKYFGPFDDNDYNNGYDKYDDCNNGRDDYNSCDCHHC
                     CPPSCVGPTGPMGPRGRTGPTGPTGPTGPGVGATGPTGPTGPTGPTGNTGNTGATGLR
                     GPTGATGATGPTGATGAIGFGVTGPTGPTGATGATGADGVTGPTGPTGATGADGITGP
                     TGATGATGFGVTGPTGPTGATGVGVTGATGLIGPTGATGTPGATGPTGAVGATGIGIT
                     GPTGPTGATGADGATGVTGPTGATGATGADGVTGPTGATGATGFGVTGPTGPTGATGI
                     GITGATGLIGPTGATGATGATGPTGVTGATGAAGLIGPTGATGATGADGATGATGATG
                     ATGPTGADGLVGPTGATGATGADGLVGPTGPTGATGVGITGATGATGATGPTGADGLV
                     GPTGATGATGADGVAGPTGATGATGNTGADGATGPTGATGPTGADGLVGPTGATGATG
                     LAGATGATGPIGATGPTGADGATGATGATGPTGADGLVGPTGATGATGATGATGPTGA
                     TGATGATGPTGASAIIPFASGIPLSLTTIAGGLVGTPGFVGFGSSAPGLSIVGGVIDL
                     TNAAGTLTNFAFSMPRAGTITSISAYFSTTAALSLVGSTVTITATLYQSTAPNNSFTA
                     VPGATVTLAPPLTGILSVGSISSGIVTGLNIAATAETRFLLVFTATASGLSLVNTVAG
                     YASAGIAIN"
     gene            9782..10543
                     /locus_tag="PC471_002948"
     CDS             9782..10543
                     /locus_tag="PC471_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase"
                     /protein_id="HDF2790058.1"
                     /translation="MKNKIEISMNNFVKNYHKSNNITTKWQELLFAYASAEDDMFQKL
                     KSVVGTSHAMPKDFLENGKTIITYFIPFDESVNNSNIGGFECSEIWARAYIETNMLIS
                     NLNDFLCEELEKMGANSYAIPGSYNFDSEKLVSDWSQRHVAFIAGLGTFGLNNMLITE
                     KGCCGRIGSIITSLELEPTKRPENEYCLYKHNNSCKKCVNKCVKEALKEDGFNRNKCY
                     EMCMINEKKYTYIGGCDICGKCIVGVPCSVINPSR"
     gene            10852..11130
                     /locus_tag="PC471_002949"
     CDS             10852..11130
                     /locus_tag="PC471_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2790059.1"
                     /translation="MSYKKAIHILPEELLELIQEYVDGECIYIPRKSNNKKEWGSNTP
                     TREELSIRNMQIYEDYQVGYDLQYLSEKYYLSLKSIQRIVLQEKRKDI"
     gene            11423..11998
                     /locus_tag="PC471_002950"
     CDS             11423..11998
                     /locus_tag="PC471_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1062 domain-containing protein"
                     /protein_id="HDF2790060.1"
                     /translation="MKKITWEVEYISQLPVIRYCKKCGKKTEYICSGLFRVNAQHKYL
                     DIWLIYKCSNCDSTWNSTIYSRVNPKSLNPEILEQFHTNDYNLVKQYAMNMELLHRNG
                     AEVGIPEYKIVGQEININDLTQVHIISKYPCQLKVSTLLREKLGLSRKVFDEMLDCGI
                     IRSNSGVDLKKGKLNYEIMLDIGKEKQKSSN"
BASE COUNT         4618 a         1520 c         2236 g         3757 t
ORIGIN      
        1 caaagttatt tgaagactcg ctatcaataa aattttatag tctataagaa aattaagatt
       61 atgagtttta taattttgat atagggatac tattttcata aatattacaa catgtagaag
      121 tatatgtcta ttattgttga attttcaatg atatattaag gttctaatat attatacaaa
      181 ataaaaacat taattcaact taaataattt taagtattat atgtgttttg attaaagaaa
      241 actaaaattt taaggaaaaa taacatattt gtattaaatt ttattgaaat gattaaattt
      301 atttacttaa tgtaaattat tagctaaaat tgtattaaat aattaaggag gtgagtagct
      361 ctggatgaaa tagatttaaa aataattaat tctctaaaaa agaatagtag ggcttcaacc
      421 tctgaaataa gcagacaaat taatttatca gtaccagcag tctcagagcg tataaggaaa
      481 attgagcagt caaatttaat tcaaaagtac actattcaaa ttaatcggga aaaaacaaaa
      541 tataagcttt tggcttttat atttatcaat actgatttat ctaaaagtat gaaagaattt
      601 aagaaaacta tattggaata tgatactgta ttagaatgtc atcatatttc aggagagtat
      661 gattacttaa taaaagtatt agcagaagat acaagtaatc ttgaagagtt tatatcagtg
      721 actttgaagg aaattggggg gattcaaaaa gctaatacaa ccattgtttt atctacaatt
      781 aaagaagaat ttaatacaat aaaagatatt ttttaaataa tttactagta aagtgttaat
      841 tatattttag gaggaaaata gaatatgaaa gttttagttt ttttagcaaa aggttttgaa
      901 actatggaat ttagtgtatt tgtagatgtt atgggatggg ctcgtaacga ttatgggcat
      961 gatattgatg tagtgacttg tggatttaaa aaacaagtta tgagcacatt taatatacaa
     1021 gtattagttg ataagacgat agaagaagta tgtgttgatg attatgatgc tctagctatt
     1081 cctgggggat ttgaagaatt tggattttat gatgaagcat atgattcatc ttttttaaat
     1141 ctaataaggg agtttaattc aaaagaaaaa ataatcgctt ctatttgtgt ggcagcattg
     1201 ccagttggaa aaagtggtgt tttaaaaaat cgtaaagcta ctacatatca tttgaaaaat
     1261 ggtaaaagac aaagacagtt aagtgagttt gatgtaaatg tagttaatga accaattgtt
     1321 gtagataaaa atattataac ctcatattgt ccagaaacag ctccacatgt agcttttaag
     1381 cttttagaaa tgttgacatc taaagaacaa atggatgaag ttaaattagc aatgggattt
     1441 aaactgtaat ggtttataga ttttctaagt aatcagaacc ccagtcacac atggaatcta
     1501 aaattggaat aatactcttt ccaaaatcag atagcgagta ttctactttt ggaggaacaa
     1561 caggataaac cactcttata attaaaccat ctgattctaa ttcacgtaat tgttgagtta
     1621 acatcttagg cgttgcttta ggaattaatt tttttaattc attgaacctc aatatagtgt
     1681 ctttaagatg ccaaagtata agtgtcttat atttaccacc aatcaatgca agtgttgctt
     1741 ctattgggca actgtaatta tctaaacatt ttttcatttt tgggctcctt tatttatttt
     1801 aaagtatctt tttaggtact atattacaaa aaagtgcatt attgtaatat agtaccatag
     1861 tgataaaatt atagcatagg ttaaataaat catcaactta gcctaataaa acaataggag
     1921 gtaaataaat gaatttcgtt gaacttgcaa aaaaaagata ttcttgccgt aattatcaag
     1981 atagaaaggt tgaaaaagaa aaacttgaga aggttttaga tgttgcaaga atagctccaa
     2041 ctggtggtaa tcgtcaacct caaagattga ttgttattca ggaaaaagaa ggtataaata
     2101 aactttctaa ggctgccaat atatatgatg ctccattagc aattcttgta tgtggagata
     2161 aagacaaagt ttggacaaga ccttttgatg ggaaacaact tacagatatt gacacaagta
     2221 tagttacaga ccatatgatg ttacaggcaa ctgaacttgg attggcaagt gtttgggtgt
     2281 gctattttaa tccagatata attagagaag agtttagttt gccagataat ctagaaccaa
     2341 taaatatatt attgatggga tatgaatcta agataccaga gagcccagag agacatgaaa
     2401 aaactcgtgt tcctttaagt gaaattgttt cttatgaaac tctttaatta taaaattaag
     2461 ttattcatag gataaatgta ataaagtgtt tattataaac aaaattcaaa ataaataata
     2521 tctacatata attaatttta gtgaagtata tctagtttaa ctagaatgta tacttcacta
     2581 atttatttat tcaaaatatt tatgaggatt ttaatatatt aactaaaata tatcaattga
     2641 attaactttt aaatctggaa aagcacttga aatatagtgt tttatgtcat ttaaatggtc
     2701 ttgaatttgc ttttctgata ttttatcagg tccatataca aaatacataa tatttttagt
     2761 atcttttgtt atagctgttc gattatcagg gccattaaga atactagata atataccaat
     2821 attatctgat aaaaataaat catcttttgt taaaatatgt tctttaccac ctataccata
     2881 aaaatgttca cttccatttg ctaattctat tttcaaaggt aattccattt tatcaaggtc
     2941 atgacctgct gttagaagaa gatttttcaa ttctgctaaa aacattgact caattgttac
     3001 acctacatct gggatacctt ttgattttaa tagaatagat tctaattgaa gcaatacagg
     3061 gtaagttttc ttgaattttt tataatactt tatataactt gaaacaggct ctgttttaat
     3121 tttttcttta cgattgtatt ctgggtgttg ttctataata tttttaattg tagagttttt
     3181 aagaactaaa aaattttctt tattaggttt tgaatgtgta atatttataa ccataactcc
     3241 aaatttaata ttgggataaa tttctttaac tttatgtgat atttctaaca taatatagac
     3301 tcctttttat tttatatttt atgatataat cttagcaaat taaaagtgga ttttgtagtt
     3361 ccatttttat agtaatttta tagaaccact tttggaggta ttagaatgtg gattacgatt
     3421 aatagaaata gtaatatatc attatcaaga caaatttatt gtgagataaa agatatgatt
     3481 ctggatggaa gcttaaagtt tgagcaaaaa ctgccatcat ctaggacttt atcaaaagaa
     3541 ctatctgtat ctcgtaatac agttttagat gcatataatc aattaattgc agaaggatat
     3601 ttggaaacaa ggaatggata tggaacaatt gtagctaaag gaatatcagg gcataaagtt
     3661 gattttaagt taaatgatac ttttagagat gaaaaagatt tacatactaa taaacaaaaa
     3721 aattatatag attttcgttc aggagttcca gaattaaaac tatttccacg caaagaactt
     3781 tcaaagctat ataaacaaat actgtatgat ttatcagatt cggatttcag atatggaagt
     3841 acagctggtg tatgggtttt aagagtggaa atatctaagt atttatttcg tactagagga
     3901 attagatgta atcccaaaaa cattatgatt gtttctggtt caacacaagg tttatcttta
     3961 atttcacagc ttttatacaa aagtaataaa aaggttgctg tagaagaccc tattcataaa
     4021 ggcttattaa aggttatttc atctattgga tattcagttg ttggaattaa agtagatgat
     4081 aagggaatga ataccaactt aataaaatca ataaagggga tttcttttgt ttatactaca
     4141 ccttctcatc aatacccatt aggagggatt ttacctattc aaagaagact agagcttatt
     4201 aaatgtgcaa cagagaatga ttactatata attgaagatg attatgatag tgaatttaga
     4261 tatgagggtc aaccaattag ttctctttat gaacttagtc caaataaagt aatttacata
     4321 ggttctttta gtaaaatatt atctccagct attcgtgttg gatttatgtt attaccagac
     4381 aatattttag aaagctataa aaccttaaaa atgtactctg atgttcatac agaaacaata
     4441 ttgcaacatg tattagctga atttattgga aatggaggtt tagagaagta catatttaga
     4501 atgaagaagt tgtacaatag aaaacgtaag tacctcataa aagttttaaa cacggaattt
     4561 aaaaatgaat ttaaaataaa aggtgaagca gcagggcttc atatagttgc tcatttctat
     4621 aatgttaaat ttacggaaag tttaattcat aaaatttttg aagcaggtat aaaagtttat
     4681 cctatacaac aatatgcaat tgagaatata ttagaatatg actatgaaat tatactaggt
     4741 tatgctcatc ttagttttga tgaaattgaa aaaggtgtaa aaatactcag caaaataata
     4801 catatgaatc aaggtacata gataaaacat gtagcaatct acatataata gatgtaatta
     4861 agtttaaact ttaaaagaaa tatatattaa tatttttgta taaaaatacc tgatattttt
     4921 ttatcgatac tttattgcta tacactaaat taagatagca atttatatta agaaaaaact
     4981 ttttgcatag gttataatac ttaaaaggag aggatagagt tgaaaacaaa tacagatgtt
     5041 attatggaga taattgatta tatagaagaa aatcttacag aaaatctaaa tttagataaa
     5101 atagcttata ttgctaatta ttcaaaatac catttacaca gaatgtttac aaatgttgta
     5161 ggatgtacta tccatcaata catacagagg aggagactta ctgaagcagc tagacaactt
     5221 atatttacag acaaatatat tatagacata gctattacag taggatattc tacacatcaa
     5281 tcttttacac tagcatttag aaatttatat aaagaatctc cacaagtata tagaaataaa
     5341 gatgaatttt atcctattca acagagattt gttttaaatc taaatcatca aattcaaagt
     5401 gaaaaatgta tttcttatgc aaatatgaat tataaacaaa gaagagatga aatcaattct
     5461 tatctgaact ttaatattaa ttgtgaggtg aaagtagcat gagtttagtg ccacatgtaa
     5521 ttgaacaaac aggtcaaggg gaaaggtctt atgatatata ttcaaaatta ttaaaagata
     5581 gaattatatt tataggagaa gaaattaatg atgctattgc aagtttagta gtagctcaat
     5641 tactgttttt agaatcagaa gacccagatt cagatataat tatatatata aatagccctg
     5701 gtggctctgc tacatcagga tttgctatat atgatactat gaattatgtc agatgtgatg
     5761 tatcgactat ttgtattggt atggctgcaa gcatgagtgc ttttttactt gcaggaggaa
     5821 cacatggaaa aagatgtgct ttacccaatt cagagattat gattcatcag ccaatgggag
     5881 gagctaaagg tcaagcaaca gatgtaaaaa tagctgtaga taatatattg aaaataaaag
     5941 aacgattaga taaaattctt tctgaaaata caggtaaatc aatagaagat attaggagag
     6001 atacagatag agataatttt atgactgcac tagaggctaa agagtacggg ctgattgatt
     6061 atataatgaa taggaatgaa taaagtatca aagtatcata ttaaaatgtc tgttttacaa
     6121 atatatatat aatattaaat aagagtgtct caaaatgaac tttttagttt atgggacact
     6181 cttttttgta tgaaattaaa tatggtttta tcatttcgac aattattacc aaatttcaag
     6241 tttaataaat tctactataa aaaatacata aaagataagg ttctttagta actttctgtc
     6301 gtcaaaagtt tttataaatt taataacaaa acaagagaaa ataccatata ttacttatgt
     6361 ctgcaatata tagaagacaa aggagttgta cattatgatt acaataagtt tgtgcatgat
     6421 tgttaagaat gaggaagaag ttataggcag atgcttagaa tgtgtaaagg atatagtaga
     6481 cgaaattata attgtagata ccggttctac agattccaca aaagaaatag tctcaaaata
     6541 tacagacatg gtgtacgatt tcgaatggat agatgatttc tctgctgcaa gaaatttttc
     6601 attttctaaa gcttctaaag actatactat gtggcttgat gcagatgaca taattttaga
     6661 agctgataga gaaaaattat taaagattaa agaaagcttg gatacttcca ttgatattgt
     6721 tatggcaaaa tacaatgtat cctttgatga aaatggtaac ccaactctct catattatag
     6781 agaacgattg tttaaaagaa gttctaatca taaatgggtt gaccctattc atgaagtagt
     6841 acctcttttt ggagaggtat tttattctga cattgcaata tctcataaaa agcttcatag
     6901 acaggaccct ctaagaaatc ttagaatttt tgaaaaaatg atttcagaag gaaaaacatt
     6961 ggagccaaga caccaattct attattctag agagttgtac tataatgcta ggtataaaga
     7021 agcaatagaa ggttttacta agtttttaga ctcatcaaga ggatggattg aggattgtat
     7081 cagtgcttgc agagatttag ccacatgtta ttacttaatt aatgatgaaa aaagtgcctt
     7141 atattcattg tttagaagtt tcgaatttga tgagccaaga gcagaaattt gttgtgacat
     7201 aggaaagcat atgtttgata gacaaaaata taaagaagcc attttttggt ataaggtagc
     7261 attaacaaga gataagaatg atactgatgg aggctttaag tcagatgatt gttatggata
     7321 tataccatct atacaattaa gtgtctgtta tgacaggtta ggagaatcag ataaagctat
     7381 ttattatcat gaaaaaacaa aagaaatcaa acctaatgat agtgctgttt tgcacaatga
     7441 aaattatttt agtaaattta aaaatagttg atgggtcaca atcatactaa atttgacata
     7501 ttatatatag agcttaaaag ctcaaatata tcaggaggtg cttttaataa tgagtagaaa
     7561 taaatatttt ggaccatttg atgataatga ttacaacaat ggctatgata aatatgatga
     7621 ttgtaacaat ggtcgtgatg attataatag ctgtgattgc catcattgct gtccaccatc
     7681 atgtgtaggt ccaacaggcc caatgggtcc aagaggtaga accggcccaa caggtccaac
     7741 gggtccaaca ggtccaggtg taggggcaac aggcccaaca ggaccaaccg gtccgactgg
     7801 tccaacagga aatacaggga atacaggagc aacaggatta agaggtccaa caggagcaac
     7861 aggggcaaca ggcccaacag gagcgacagg agctatagga tttggagtaa caggtccaac
     7921 aggcccaaca ggagcgacag gagcaacagg agcagatgga gtaacaggtc caacaggtcc
     7981 aacgggagca acaggagcag atggaataac aggtccaaca ggagcaacag gggcaacagg
     8041 atttggagta acaggtccaa caggcccaac aggagcaaca ggagtaggag taacaggagc
     8101 aacaggatta ataggtccaa caggagcaac aggaacacct ggagcgacag gcccaacagg
     8161 agcagtagga gcaacaggaa taggaataac aggtccaaca ggtccaacag gagcaacagg
     8221 agcagacgga gcaacaggag taacaggtcc aacaggggca acaggggcaa caggagcaga
     8281 tggagtaaca ggtccaacag gggcaacagg agcaacagga tttggagtga caggcccaac
     8341 aggtccaaca ggagcaacag gaataggaat aacaggagca acagggttaa taggtccaac
     8401 cggagcaacg ggagcaacag gagcaacagg cccaacagga gtaacagggg caacaggagc
     8461 agcaggacta ataggaccaa ccggggcaac aggagcaacc ggagcagatg gagcaaccgg
     8521 agcgacaggg gcaacaggag caacaggtcc aacaggagca gatggattag taggtccaac
     8581 aggagcaaca ggagcaacag gagcagatgg attagtaggc ccaacaggtc caacaggggc
     8641 aacaggagta ggaataacag gagcaacagg agcaacagga gcgacaggtc caacaggggc
     8701 agatggatta gtaggtccaa caggagcgac gggagcaaca ggagcagatg gagtagcagg
     8761 tccaacagga gcaacaggag caacaggaaa tacaggagca gatggagcaa caggtccaac
     8821 aggggcaaca ggtccaacag gagcagacgg attagtaggt ccaacaggag caacaggagc
     8881 aacaggatta gcaggagcaa caggagcaac aggcccaata ggagcaacag gtccaacagg
     8941 agcagatgga gcaacagggg caacaggagc aacaggtcca acaggggcag atggattagt
     9001 aggtccaaca ggagcaaccg gagcaaccgg agcgaccgga gcaacaggtc caacaggagc
     9061 aacaggagca acaggagcaa caggtccaac aggtgctagt gcaataatac cttttgcatc
     9121 aggtatacca ctatcactta caactatagc tggaggatta gtaggtacac caggttttgt
     9181 tggctttggt agttcagctc caggattaag tatagttggt ggagtaatag accttacaaa
     9241 cgcagcagga acattgacta actttgcatt ttcaatgcca agagctggaa caataacatc
     9301 tatttcagca tacttcagta caacagcagc actttcactt gttggctcaa cagttacaat
     9361 tacagcaaca ctttaccaat ctactgcacc aaataactca tttacagctg taccaggagc
     9421 gacagttaca ctagctccac cacttacagg aatattatca gttggttcaa tttctagtgg
     9481 aattgtaaca ggattaaata tagcagcaac agcagaaact cgattcttac tagtatttac
     9541 tgcaacagct tcaggtcttt cattagttaa tactgtagca ggatatgcaa gtgcaggaat
     9601 tgcaataaat tagattagat aagaaaaggc ttctaatatc gtattgatat taggagcttt
     9661 tttaagaatg attgatgaac atagttttaa tattaattag tgcgcatatt ataaaatact
     9721 attatataat ataaaataat aacattaaat tggaaatata gaattatcta ggggtgatac
     9781 tatgaaaaat aagattgaaa tatcaatgaa caactttgta aaaaattatc ataaatcaaa
     9841 taatataact acaaagtggc aagaactttt gtttgcttat gcatctgctg aagatgatat
     9901 gtttcagaag ctaaaaagtg ttgtaggaac atctcatgct atgccaaaag attttctgga
     9961 aaatggaaaa actataatta catattttat accttttgat gaaagtgtaa ataatagtaa
    10021 tattggaggg tttgaatgtt ctgaaatatg ggcaagagcg tacatagaaa ctaatatgtt
    10081 gatttcaaat ttaaatgatt tcttatgtga agaactagag aagatggggg ctaattcata
    10141 tgctattcct gggtcgtata attttgacag tgaaaaattg gtaagtgact ggtcacaaag
    10201 acatgtagca ttcatagcag gtcttggaac ttttggttta aataatatgc ttattacaga
    10261 aaaagggtgt tgtggaagaa taggaagcat aatcacaagt ttagaattag agccaactaa
    10321 aagaccagaa aatgaatact gcttgtataa acataataat agttgcaaaa aatgtgtcaa
    10381 caaatgcgtg aaagaagctt taaaagaaga tggatttaat aggaataagt gttatgaaat
    10441 gtgtatgata aatgaaaaaa aatacactta tataggtgga tgtgatattt gtggaaagtg
    10501 tatagttgga gttccttgtt cagtaataaa cccatcaaga taatggattt aagttgacta
    10561 ataattttaa agatagttta taatgaaaaa attttagtta tcaaaataaa tataatataa
    10621 aaactaaata aatgtgttta aaaaatggat aaataatttg tgttgattga caaccagtta
    10681 tactgttgat agacttatat gtatcgctgt gatatataag gagggttgag attttatgtt
    10741 tgatttaatc tttatttttg catagcaagg gctattgcaa ctatgaaatt taatatataa
    10801 atagcccttt gctattccta atgaaaaatt actatttaat aggagagcaa aatgagctat
    10861 aaaaaagcaa tacatatatt accagaagaa ttacttgaat taatacagga atatgtagat
    10921 ggtgaatgta tctatattcc tagaaaatct aataataaaa aagaatgggg aagtaataca
    10981 cctactcgtg aggagttgtc aattagaaat atgcagattt acgaagacta tcaagttgga
    11041 tatgatttgc aatatttatc ggaaaagtat tatttatctt tgaaaagtat ccaaagaatt
    11101 gtattacaag aaaaaagaaa agatatctaa ggatgagcca gtatagtttt ataattatac
    11161 tggctcattt tcttttgata aaagtgtttg aggtatagtt tcttttaagt ggatgatata
    11221 atttaagtaa tttcaataac ttatacagta gctaacaaag ctggttgtaa agtatctaat
    11281 aagaagtagt agctttttat atgtttgaaa ttaaaattta ttaaaattag gaggtcttgt
    11341 tttgaaaaaa atacttgatt atattccacc ttttcataac tttctattaa tttagtaatc
    11401 ttatagaaag gaaattgcat ttatgaaaaa aattacttgg gaggtggaat atatctccca
    11461 gttacctgta attaggtatt gtaaaaaatg tggaaaaaag actgaatata tttgttcagg
    11521 tttatttagg gttaatgctc aacataaata tttggatata tggttaatat acaagtgttc
    11581 aaattgtgat tcaacatgga actcaactat atattctcgt gttaatccaa aaagcttaaa
    11641 tccagaaata ctagaacaat tccatacaaa cgattataat cttgttaagc aatatgctat
    11701 gaatatggaa cttttacata gaaatggagc agaagttggt attccagagt ataaaatagt
    11761 aggacaagaa attaatatta atgaccttac acaagttcat attattagta aatatccatg
    11821 tcagttaaag gtatcaacat tattacgtga gaaactaggt ttatctagga aggtatttga
    11881 cgaaatgttg gattgtggga ttattagaag taactcagga gttgatttaa agaaaggtaa
    11941 attaaattat gaaattatgt tagatatagg caaggagaag caaaagtctt caaattgatt
    12001 agaataacta acttaatata ttttcaggaa catcatttaa aaaaatagtg gctttaaaaa
    12061 atttaaagcc actatttttt aatatgtttt atattaatat ttaaaatata gtgtgtattc
    12121 ttcaaaatta t
//
LOCUS       DAMJYR010000113        12128 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.116, whole genome shotgun sequence.
ACCESSION   DAMJYR010000113 DAMJYR010000000
VERSION     DAMJYR010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12128)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12128)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12128
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.116"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            79..2190
                     /locus_tag="PC471_002951"
     CDS             79..2190
                     /locus_tag="PC471_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding glycosyl-hydrolase Cwp19"
                     /protein_id="HDF2790061.1"
                     /translation="MKKISILVLSLIMTLTMCSVSSFADSSNDKEMRAAWISTVYNLD
                     WPKTKNNEAKQKKEYTDLLDKLKSVGINTAVVQVRPKSDALYKSNINPWSEYLTGTQG
                     KDPGYDPLPFLIEEAHKRGMEFHAWFNPYRITMADESIDKLPANHPAKKNPSWVVKHG
                     NKYYYDPGLPEVRKYIVDSIAEVVQNYDIDGVHFDDYFYPGVSFNDTATYQKYGKGQN
                     KDDWRRENVNTLLRDVKASIKSIKPNVVFGVSPAGIWRNKSSDPTGSDTSGNESYVGT
                     YADTRAWIKQGLIDYVVPQLYWPIGLKAADYSKLVAWWANEVKGTNVDLYIGQGIYKQ
                     GQSSYGGQNIAKEIVQQVTLNRKYSEIKGSMYFSAKDIANSTSIQKDLKSLYSSSEEP
                     VTPPSNVKVEKLRGDERYDTAVAISKKGWATNSDTVVLVNGYSIVDGITSTPLATSND
                     APILLVNKDNIPTSTKNELKRLNPSKVILIGGNNSIGDKVESEIKDTLSNVSINRVGG
                     SDRYSTSLMIAKELVKTNPVEKLYITSGTGEADSLSIASKAGEEKQPIVLVSKDNVSD
                     EVYNWISDLKVKDAYFIGGNLSISDSVINKLDKVITNDVSKNRIAGENREETNGKVIQ
                     KFYPNAEYSSMFVSKSNQLVDALTSGPLAAKLKSPVVMLGNSVTSAQKTALEPKKTTL
                     VYEAGDGINQNTLNTFLNLVK"
     gene            complement(2388..3353)
                     /locus_tag="PC471_002952"
     CDS             complement(2388..3353)
                     /locus_tag="PC471_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HDF2790062.1"
                     /translation="MSKLKKFVILLAFLVVIFPISVYGYFYYKLSAIHDSSISSDLLD
                     NNDHKNEDGIINILLMGTDARPNEDSSRSDAMMILTIDNKHNDIKLTSLARDSYVDIP
                     GHGKQKLTHAYAYGQADLLIQTIEENFNIDIQNYACVNFESFMYIIDAIGGVEVTVEK
                     GEIRELNKFIPETYKWNKSDDKGSIQYIRNSGKQTLNGYQALSFARIRHNDTAFARDG
                     RQRQIIQAIIKKTETLPVTKYPGLLDAVLPYVKTNMKPNAILSLGAQVLKMGDLNIKQ
                     FEFPIDDEIHSTGGIYGKAGWVLRFDPDTLDILHDFIFNDIEFKQ"
     gene            complement(3638..4594)
                     /locus_tag="PC471_002953"
     CDS             complement(3638..4594)
                     /locus_tag="PC471_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HDF2790063.1"
                     /translation="MSGLKKFVIFLALLVIAFPVSIYGYVFTKLKTIQDDSINTEALS
                     SNDYKNEENITNILLLGTDARPGEEVSRSDAMMILTIDNKNKSLKLTSLNRDTYVNIP
                     GRGEQKLTHAYVYGQANLLIETIEENFQLDIQNYAVVDFFSFMDIVDALGGVTVTVDK
                     SEIRELNKFIPETYKWNKNDNKGSLKYIRNSGEQKLNGYQTLAFSRIRKNDSTDERDR
                     RQRSVIQSLINGVKDLPVTKYPNLVNTILPYVKTNMNPNEIISLGKELLSIGNLNLKS
                     MEFPLSTENGRKIGNAGYVIPFEEYELDAMHDFIFKDIMVED"
     gene            4962..5750
                     /locus_tag="PC471_002954"
     CDS             4962..5750
                     /locus_tag="PC471_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDF2790064.1"
                     /translation="MIKLIATDLDGTLLDEKSEINPEFYKVFKKLRERGIMFSAASGR
                     QYQNLIKKFEDIKDDMMFISENGTLVVYKGKEILSNPLNKELVNEIIETTRSIKGKKI
                     VMSGKKYAYIESKDEAFIQEVSTYYAKFKIVEDLTKVEGDILKIAVFDFKGAEHNNNI
                     YFEKFSDRAQVCISGVEWLDLTAKGANKGSAIKKVQKMLDIKYEETMVFGDQLNDVEM
                     MKSAYHSYAMENANEHLKQIARFRAKRNTENGVVDKIKEVIKIG"
     gene            complement(5873..6343)
                     /locus_tag="PC471_002955"
     CDS             complement(5873..6343)
                     /locus_tag="PC471_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4358 domain-containing protein"
                     /protein_id="HDF2790065.1"
                     /translation="MKKILILFFSIILSLGMVSCSSSGDYKNVSTSKIKKALKSSDLL
                     IKESKIFDAKDFNYFNDVEEDIIQGFVINSSEKLSLEDIIVIKTDEVDKIYKTLENYK
                     KDMIQAPFGEGHGEKINSEIANNTIIEKAGRYVYLISAENATQIENKILNIIKK"
     gene            6482..7126
                     /gene="uppS"
                     /locus_tag="PC471_002956"
     CDS             6482..7126
                     /gene="uppS"
                     /locus_tag="PC471_002956"
                     /EC_number="2.5.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="di-trans,poly-cis-decaprenylcistransferase"
                     /protein_id="HDF2790066.1"
                     /translation="MRIPKHVGIIPDGNRRWASQNGMTKDKGYESGIDPGLESYRLCR
                     DLGCEEVTFYGFTTDNTRRPSVQTKAFTKACIDAANILIDEGAALLVVGNTKSPMFPE
                     ELLPYTNRIKAKEGQIKANLLVNYGWYWDLNNISGVENVNKNNIQDYIQSFDVSRLDL
                     IIRWGGRRRLSGFLPVQSIYADFYVIDNYWPDFKSDDIKDAFSWYDTQDVTLGG"
     gene            7169..8074
                     /locus_tag="PC471_002957"
     CDS             7169..8074
                     /locus_tag="PC471_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HDF2790067.1"
                     /translation="MKKKLLDGKITLIICKSVKIYTKKGEEMSKGNNNNNSRNKSKKT
                     SHLNRKKRKLNKKKLAVLICFTVLFLFIAFKATQGVVALVKSMDKSNKTNQQQNVNSE
                     QFDLGNEEENKKKKYTVFIDPGHGGNDKGTESKTSNRYEKDLNLQIAKKLANKLSKQK
                     DIQVVVSRTDDTYISLKDRAILANNSSADVLVSIHLNAEKNGNTATGIETWYRNKATD
                     GSKELAQTVQSTIVSYVKVRDRGIVENNFEVLRESNMPAILIECGFLTTPSEEQKIIN
                     EKYQDQLAEGIVQGVLSYLDSKGNK"
     gene            8389..9936
                     /locus_tag="PC471_002958"
     CDS             8389..9936
                     /locus_tag="PC471_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HDF2790068.1"
                     /translation="MNKNSMTRIYSLNKIIFIIIGILGIVLLTRHIEVDIKVLILILL
                     TLFSINVFISYLKIKLYEDKINLGLINNSDKFINITKDENFLKHVQNYIISNEKENCV
                     MACFDLCRLKLINDIYGYELGDEVLNNILSNLKNYFGKEAIYGKLKSDVFVLIIKLEN
                     REQKIPYLVNLIKNKIVILSQIDSFKMDINIEASIGIYKFNNNEIDIKKAIDNADMAR
                     LKSKGLKHIEYVEFDNAMEEEIQSIMKIERDLFLAIKNKEFVIHYQPKVDSSTGNIIG
                     SEALIRWLHPSLGMVGPNKFIPIAEKNGLINNIGRWLIQEVFITINKWISEGINVLPV
                     SINLSRVELYKNDLVDFFKLMFNTYNIPKELIELEITETTALRDVKFISERLYEIKSL
                     GMNISLDDFGVGNSNFINLKGIPLDIIKIDRSLILDIVTNTKTKFMVKAIVNLSHDLN
                     VTVICEGVEDMHQVKVLSELGCNFIQGYVFFKPLDEMNYKKLLTDGSYINLKDTLLDK
                     CMTVEEE"
     gene            9939..10337
                     /locus_tag="PC471_002959"
     CDS             9939..10337
                     /locus_tag="PC471_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HDF2790069.1"
                     /translation="MISEDVDYSIRIIRAIFNNGEDKPIDAKMILKSEDIPILTLYRL
                     LRKLCANNILTFNKEDGKTYMLNVSLDTISLRTIVEVIDGKIYINKCTRYVSNCSNCL
                     KGCKVNRELLEIEKEIYKLLSRKSIRQILE"
     gene            10377..12110
                     /locus_tag="PC471_002960"
     CDS             10377..12110
                     /locus_tag="PC471_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HDF2790070.1"
                     /translation="MDDISQDNFLLSKEYENSLDVDTKKASGIYYTPKIIVDYIVKKT
                     LKNHDIIKNPYPRILDISCGCGNFLLEVYDILYDLFEENIYELKKKYDENYWTVDNIH
                     RHILNYCIYGADIDEKAISILKDSLTNKKVVNDLDESDIKINLFCCDSLKKKWRYKFD
                     YIVGNPPYIGHKKLEKKYKKFLLEKYSEVYKDKADLYFCFYKKIIDILKQGGIGSVIT
                     PRYFLESLSGKDLREYIKSNVNVQEIVDFLGANIFKNIGVSSCILTFDKKKTKETYID
                     VFKIKNEDICINKFETLEELLKSSKFEHFNINQRLLSDEWILVNKDDETFYNKIQEKC
                     KYSLEDIAISFQGIITGCDKAFILSKDDVKLNLVDDKFLKCWIKSKNINKYIVDKSEY
                     RLIYSNDIDNENTNKRILDEIIGLYKTKLENRRECKSGIRKWYELQWGREKLFFERKK
                     IMYPYKSNENRFAIDYDNNFSSADVYSFFIKEEYLDKFSYEYLVGILNSSVYDKYFKI
                     TAKKMSKNIYDYYPNKVMKIRIFRDNNYEEIENLSKQIISILLNKSIDKGKVEKLQIK
                     MDNLIMDSLGI"
BASE COUNT         4846 a         1314 c         1956 g         4012 t
ORIGIN      
        1 agataatatg aattttagat atacttaata agaagtatat tgtagaaaaa ataaatttaa
       61 aaaattaagg gggatgaaat gaaaaaaata agtatattag tattatcatt gataatgaca
      121 cttactatgt gtagtgtaag ttcatttgct gattcatcaa atgataagga aatgagagct
      181 gcttggatat ctacagttta taatttagac tggcctaaga ctaagaataa tgaagccaag
      241 caaaaaaagg aatacacaga tttattggac aaattaaaaa gtgtaggaat aaatacagca
      301 gtggttcaag taagaccaaa atcagatgct ttatataaat caaatataaa cccatggtct
      361 gaatatctta ccggtacaca aggtaaagac cctggatatg acccattacc atttttaata
      421 gaagaagcac ataaaagagg aatggaattt catgcatggt ttaatccata tagaataact
      481 atggcagatg agagcataga taaattgcca gcaaaccatc cagcaaagaa aaatccaagc
      541 tgggtagtta aacatggaaa taagtattac tacgacccag gtttaccaga agttagaaag
      601 tacatagtgg atagtatagc agaagtagtt caaaattacg atatagatgg agttcatttt
      661 gatgattact tctatccagg agtaagtttc aatgatactg caacatacca aaaatatgga
      721 aaaggacaaa ataaggatga ttggagaaga gaaaatgtaa atactctact tagagatgta
      781 aaagcgtcta taaagagtat aaagcctaat gttgtatttg gtgtaagccc agcaggtata
      841 tggagaaata agagtagtga cccaacaggt tcagatacat caggtaatga aagttatgta
      901 ggtacatatg cagatacaag agcatggata aaacaaggat tgattgacta tgtagttcca
      961 caactttact ggccaattgg tcttaaagca gcagattatt ctaaattagt ggcatggtgg
     1021 gctaatgaag taaaaggaac aaatgtagac ttatacatag gacaaggtat atataagcaa
     1081 ggtcaatcga gttatggtgg acaaaatata gcaaaagaaa tagtacaaca agtaacatta
     1141 aatagaaagt atagtgaaat aaaaggtagt atgtatttct cagctaaaga tatagcaaat
     1201 agtacttcta tacaaaagga tttaaagagt ttatattcta gtagtgaaga accagttact
     1261 ccaccatcta atgttaaagt ggagaaatta agaggcgacg aaagatatga cacagcagta
     1321 gcaataagta aaaaaggttg ggctacaaat tcagatacag tagtattagt aaatgggtac
     1381 tctatagttg atggtataac atcaacacca cttgctacat caaatgatgc tcctatacta
     1441 ttggtaaata aggataatat acctacatcc actaagaatg agttaaaaag acttaatcca
     1501 agtaaagtta ttttaattgg aggaaataac tctattggag ataaagttga gagtgagata
     1561 aaagatacat tgtcaaatgt atcaataaat agagttggtg gttcagatag atatagtaca
     1621 tctttaatga tagcaaaaga attagtaaaa actaatccag tagaaaaact atatataaca
     1681 agtggtacag gtgaagcaga ttctctttct atagcttcaa aagcaggaga agaaaagcaa
     1741 ccaatagtat tagtatcaaa agataatgta agtgatgaag tttacaattg gatatcagat
     1801 ttgaaagtaa aagatgctta ttttataggt ggtaatctaa gtatatctga ttcagtaata
     1861 aataagctgg ataaggtcat tacaaatgat gtatctaaaa atagaatagc tggagaaaat
     1921 agagaagaaa caaatggaaa agtaatacaa aaattctatc caaatgctga atattcatca
     1981 atgtttgtaa gtaaaagtaa tcaattggta gatgcattaa catcaggtcc tttagcagct
     2041 aaattaaaat caccagtagt tatgcttgga aatagtgtaa catctgctca gaaaactgct
     2101 ttagagccta agaaaactac tctagtatac gaagctggag atggtataaa tcaaaacaca
     2161 ttaaacactt tcttaaactt agttaagtaa taaaaaagtt aatttaaaat acagagtatt
     2221 ttataaatgt agattaaaat gaaaattatc atgataaatt agaaaataaa aaagaactat
     2281 atgcattttg taatcaagtg tacacagcaa cttgaaaata attatgcgat atagttcttt
     2341 tttagtaatt aatcttatac tttttaataa actttatgtt gaatttttta ttgtttaaac
     2401 tctatgtcat taaatataaa atcgtgtaat atgtctaaag tatctgggtc aaatctaaga
     2461 acccatccag cttttccata tataccacct gtactatgaa tttcatcatc aataggaaat
     2521 tcaaattgct taatatttaa gtcgcccatt tttaaaactt gagctcctaa agaaagtatt
     2581 gcattaggct tcatgttagt tttaacatat ggaagaactg catctaataa acctggatat
     2641 ttagtaacag gtaaagtttc cgttttcttg attatagctt gtataatctg tctctgtctg
     2701 ccatctctag caaaggctgt atcgttgtgt ctgatacgtg caaaagatag tgcctgatat
     2761 ccatttagag tctgctttcc agagtttcta atgtattgta tgctaccttt atcatcactt
     2821 ttattccatt tataagtttc aggaataaat ttatttagtt ctcgtatttc tcctttttca
     2881 acagtaacct caacgccacc aatagcatca attatataca taaatgattc gaaattaaca
     2941 caagcataat tttgaatatc aatattaaaa ttctcttcaa ttgtttgtat taataaatca
     3001 gcttgtccat aagcataagc atgagtaagt ttttgtttac catgaccagg gatgtctaca
     3061 tagctatcac gagctaatga tgtaagtttg atatcattgt gtttgttatc aatagtaagt
     3121 atcatcatag catctgacct tgaagaatct tcatttggtc tagcatctgt tcccataagt
     3181 agtatgttaa ttatcccatc ttcattttta tggtcattgt tatccaataa atcactactt
     3241 atgctcgagt catgaatagc agataattta taataaaaat atccatagac acttataggg
     3301 aatattacta ccaagaaagc cagtagtata acaaatttct ttaattttga caaaagtacc
     3361 ccttctttct tctttttcct gtatattttt taatagcata cacttaatta tattaaagta
     3421 ttgagatata ttcaatactt gtttcagtta caaaaaaaag acaatgatat aatttataag
     3481 aaaatttatc gacctatata tatagttaaa agtatatata atatattttt taaaagtaat
     3541 ttatatttta aaataaaaac acaccccata aatacaaagt aatttgcatt taagaggtat
     3601 gtatttatca tcaaattatt ggtttgtttt ggaatttcta atcttcaacc ataatatctt
     3661 taaatatgaa atcatgcatt gcatctagtt catattcctc aaaaggtatt acatagcctg
     3721 cattacctat ttttctacca ttttcagtag aaagaggaaa ctccatagat ttcaaattta
     3781 aatttcctat acttaaaagt tcttttccta aagatattat ttcattagga ttcatattag
     3841 tttttacata cggaagtatt gtgttaacta gattaggata tttagtgact ggtaaatcct
     3901 taacaccatt tattaaactc tgtataacac ttctttgtct tctatctctt tcatctgtac
     3961 tatcattttt tcttatacga gaaaatgcaa gtgtctgata tccatttaat ttttgctcac
     4021 cagaatttct tatatattta agagaacctt tattgtcatt tttattccat ttataagttt
     4081 caggaataaa tttatttaat tctcttattt cactcttatc aacagtaaca gtaactccac
     4141 ctaaagcatc aactatatcc ataaaagaga aaaagtcaac tacggcgtag ttttgtatat
     4201 caagttggaa gttttcttct atagtttcta ttaataagtt agcttgtcca tatacgtagg
     4261 catgtgttaa tttttgttct ccacgtcctg gtatattaac ataagtatct ctatttaatg
     4321 aagttagctt aagactttta tttttattat caatagtaag tatcatcatg gcatcagaac
     4381 gagaaacttc ttctcctggt cttgcatctg tacctaacaa taatatgttt gtgatatttt
     4441 cttcgttttt atagtcatta ctactaagcg cctctgtatt tatactatca tcttgtatag
     4501 tttttaattt agtaaaaaca tatccatata tactaacagg aaatgctata actaaaaggg
     4561 ctagaaatat aacgaacttt ttgagtcctg acaataatat cccctacttt ctaaattttt
     4621 taattacaca tagttaatta tattaaaaat ttgttacata agcaataaaa aacacaataa
     4681 attagcataa gtttgtcata atataattat ttttatagca ttttatagaa gaaagtgtgt
     4741 agttattatt gattttattg gaatataatt gtatgaagac ttaaattttt ataaactgat
     4801 gtgtatattg aaaaaaatat tttcctgaat gtagttatag catcttatga aaggtgcaaa
     4861 ttgtaattta aaatttttag tgtataataa gaattgaaaa ttcaaaccga taaaaattaa
     4921 taaaaaataa ttataaatta ataatttggg ggaggaaatg tatgataaaa ttaatagcaa
     4981 cagatttaga tggaactttg ttagatgaaa agagcgaaat aaatccagag ttttataaag
     5041 tatttaaaaa attaagagag agagggataa tgttttctgc tgctagtggc aggcagtatc
     5101 aaaatcttat aaaaaaattt gaagatataa aagatgatat gatgtttata tcagaaaatg
     5161 gaacactagt agtatataaa ggaaaagaaa tattatcaaa tcctttaaat aaagaattag
     5221 taaatgaaat catagagaca acaagaagta taaaagggaa aaaaattgta atgagtggga
     5281 aaaaatacgc ttatatagaa agcaaagatg aagcgtttat acaagaggtc agtacttatt
     5341 atgcaaagtt taaaattgta gaagatttga cgaaagtaga aggagacata ttgaagatag
     5401 ctgtatttga cttcaaaggt gcagaacata ataataatat ctactttgaa aagttttctg
     5461 atagagctca agtatgtatt tctggagtgg agtggcttga tttaactgca aaaggtgcaa
     5521 ataaagggag tgccataaaa aaagtacaaa aaatgttaga tataaaatat gaagaaacta
     5581 tggtatttgg tgaccagtta aatgatgtgg aaatgatgaa aagtgcctat catagttatg
     5641 ccatggaaaa tgcaaatgaa catttgaaac aaattgctag atttagagca aaaagaaata
     5701 ctgaaaatgg cgttgtagat aagataaaag aggttataaa aattggatag tcatatgtat
     5761 agatgaaaaa gattattttg aagaaatcaa aaaaatatca atgtaatttt gataaataag
     5821 tttatttatg cgaaaattac attgatattt aataaacatc aataatagat atttattttt
     5881 ttatgatatt cagtattttg ttttctattt gagttgcatt ttcagcagaa attaaataaa
     5941 catatctacc tgccttctca attatagtat tatttgctat ttcagaattg attttttctc
     6001 catgtccttc tccaaaaggt gcttgtatca tatctttttt ataattttct agagttttat
     6061 agattttatc tacctcgtca gtttttataa cgataatatc ttctagagat aatttttcag
     6121 aagaattaat tacaaaacct tgaattatgt cttcctctac atcattaaaa tagttaaaat
     6181 cctttgcatc aaagattttt gattccttta taagtaaatc agaagatttt aatgcttttt
     6241 ttatttttga tgtagaaacg tttttgtaat caccacttga agaacaacta accattccta
     6301 atgataagat tatcgaaaaa aacaatatta aaattttttt catgataaac ctccaagttt
     6361 tttaaactct gtcttaaatt agtttactac ataattatat aaaaataaat gtatttaatg
     6421 ttacagtttt gattaataat gtaatatttt gataataata cctattgaga ggtgattaga
     6481 aatgagaata ccaaaacatg taggaattat accggatgga aatagaaggt gggcaagtca
     6541 aaatggtatg acaaaagata aaggatatga aagtggaata gaccctggac tagagtctta
     6601 tagattatgt agagatttag gctgtgaaga ggtgactttt tatggattta caactgataa
     6661 tacaagacgt ccatcagtgc agacaaaagc ttttacaaaa gcatgtattg atgctgcaaa
     6721 tatattaata gatgaaggtg ctgctttatt agttgtagga aatacaaaat ctccaatgtt
     6781 tccagaagaa ttattaccat atactaatag aattaaagcc aaagaaggtc aaataaaagc
     6841 aaatcttctg gtaaattatg gttggtattg ggacttaaat aatatatcag gagtagaaaa
     6901 tgtaaataaa aataatatac aggattacat acagtcattt gatgtatcca gactagactt
     6961 gataatcaga tggggtggaa gaaggagatt aagtggtttc cttcctgtac aatcaattta
     7021 tgcagatttt tatgttatag ataattattg gccagatttt aagagtgacg atataaagga
     7081 cgcatttagt tggtatgata cacaagatgt cactttaggt ggataattaa aaatattctg
     7141 atttaaggtt gtagagtgtt ttaaattatt gaaaaaaaaa cttcttgatg gtaaaataac
     7201 actaataata tgtaaaagtg taaaaattta taccaagaag ggtgaagaaa tgtcaaaagg
     7261 caataataac aataattcaa gaaacaaatc taaaaagacc agtcatctga atagaaaaaa
     7321 gcgaaaatta aataagaaaa agttagcagt gttgatttgc tttacagttt tatttttatt
     7381 tattgcattt aaggctactc aaggtgtagt agctttggta aaaagtatgg ataaaagtaa
     7441 taaaactaat caacaacaaa atgttaactc agaacaattt gaccttggta atgaagagga
     7501 aaataaaaag aaaaaatata ctgtatttat agacccaggt catggaggca atgataaagg
     7561 aactgaaagc aagaccagta atagatatga aaaagaccta aatctacaaa ttgcaaaaaa
     7621 attagcaaat aaattatcta aacaaaagga tattcaagta gtagtatcca gaactgatga
     7681 tacatatata tcattaaagg atagagctat cttagcaaat aatagttctg ctgatgtgct
     7741 tgtttctatt catttaaatg cagagaaaaa tggtaataca gcaacaggta tagaaacttg
     7801 gtataggaat aaagctacag atggttcaaa agaacttgca cagacagtac aaagtactat
     7861 agtatcctat gttaaagtta gagatagggg aatagttgaa aataactttg aagtacttag
     7921 agaatcaaat atgcctgcta tattaataga gtgtggtttt ttaacaacac cttcagagga
     7981 acaaaaaata ataaatgaaa aatatcaaga ccaacttgcg gaaggaattg ttcaaggtgt
     8041 actttcatac ctagattcta aagggaataa gtaagttaac tttactttaa tgtgaggata
     8101 aatttaaaag tttaactgtg taaattgtat taactttatt tcgtaatgtt gcaaacattg
     8161 tttgaattta gaaactatga aattagaaat tattttatct atatctaatt tcatagtttt
     8221 ttttgcaaaa aatttatagt tataaaattt taaaaaataa atttagtata atgtaatgta
     8281 gttatttata tgtgatttta acttgcattg atttgctatc aattgtgctt tataattagt
     8341 atatataaag tttaactgta agataattta aaaaataggg gaatttatat gaataaaaat
     8401 agcatgacaa gaatttacag tttaaataaa ataattttta ttataattgg aattttagga
     8461 atagttttac tcacaagaca tattgaggta gatattaaag ttttaatttt aattttatta
     8521 accttgttta gtataaatgt atttatttca tatcttaaaa ttaagttgta tgaggataaa
     8581 attaatttag gattgataaa taattcagat aagtttataa atattactaa agatgagaac
     8641 ttcctaaaac atgttcaaaa ttatattatc agtaatgaaa aagaaaactg tgtaatggct
     8701 tgttttgatt tatgtagact caaacttata aatgatatat atggttatga gctaggggat
     8761 gaagtgttaa ataatatttt aagtaactta aaaaattatt ttggaaaaga agctatttat
     8821 ggaaagttaa aaagtgatgt ttttgtatta ataataaagc ttgaaaatag ggaacagaag
     8881 attccttatt tagtaaatct tattaagaat aaaattgtga tactaagtca gattgacagt
     8941 tttaaaatgg atataaatat tgaggcttct attggaattt ataaatttaa caataatgaa
     9001 attgatataa aaaaagctat agataatgct gatatggcaa gacttaaatc aaaaggacta
     9061 aagcacatag agtatgtaga atttgataat gctatggaag aagaaataca atcaattatg
     9121 aaaatagaga gagatttatt cttggctatt aaaaataaag agtttgtaat tcactatcaa
     9181 cctaaagttg attcaagtac aggtaacatt ataggttcag aggcattgat aagatggttg
     9241 cacccatcat taggcatggt tggaccaaat aagtttatac ctatagctga aaaaaatggt
     9301 ctgataaaca atattggtag atggctaatt caagaagttt ttattaccat taacaagtgg
     9361 atttcagaag gtataaatgt cttaccagtc tcaataaatc tttctagagt agaactatat
     9421 aaaaatgatt tagttgattt ttttaaactg atgtttaata catataatat tccaaaagaa
     9481 cttatagaat tagaaattac agaaacgaca gcactaagag atgtaaaatt tataagtgaa
     9541 aggctttatg agattaaatc tttaggtatg aatatatcat tagatgattt tggagtaggt
     9601 aattcaaatt ttatcaacct taaggggata ccactagata ttataaaaat tgataggtca
     9661 cttatattgg atatagttac aaatacaaaa acaaagttta tggtgaaagc aattgttaac
     9721 ctttcacatg atttaaacgt aactgttata tgtgaagggg tagaggatat gcatcaggtc
     9781 aaagtcttaa gtgaactggg atgtaatttc atacaaggtt atgtattttt taaacctttg
     9841 gatgagatga attataaaaa acttcttact gacggaagtt atataaatct gaaagatact
     9901 cttttagata aatgcatgac agttgaggag gagtaaggat gatttcagaa gatgtagatt
     9961 attcaataag aatcataaga gctatattta ataatggaga agataaacca atagatgcta
    10021 aaatgatatt aaaaagtgaa gatataccaa tactaacact atacagactg cttagaaagc
    10081 tttgtgcaaa caatattctt acttttaaca aagaagatgg caagacatac atgttaaatg
    10141 tgtcattgga tacaataagt ctaagaacta ttgtagaagt aattgatgga aaaatatata
    10201 taaataaatg tacgagatat gtatctaatt gcagtaattg cttaaaaggt tgtaaagtaa
    10261 atcgtgaatt gctagaaata gaaaaagaaa tttataaact tttatcgaga aaatctataa
    10321 gacaaatact agaataaaaa attttcagct atgataataa ttggaggttt tcggttatgg
    10381 atgatatatc tcaagataat tttctactgt ctaaagagta tgagaatagc ttagatgtag
    10441 atactaaaaa agcaagtgga atatattata ctcctaaaat aattgtagat tatattgtaa
    10501 aaaaaacttt aaaaaatcac gatataatta aaaatccata tccacgaata cttgatattt
    10561 cctgtgggtg tggaaatttt cttttagaag tatatgatat tttatatgac ttatttgagg
    10621 aaaatatata tgaattgaaa aagaagtatg atgaaaatta ttggactgtt gataacatac
    10681 accgtcatat attaaactat tgtatatatg gtgctgatat tgatgaaaaa gctataagta
    10741 tattaaaaga tagcttaaca aataaaaagg tagtaaatga tttagatgaa tctgatataa
    10801 agattaatct attttgttgt gatagtctta aaaagaaatg gcgatataag tttgattaca
    10861 tagtgggtaa tccaccctat attggtcata aaaaacttga aaagaaatat aaaaaatttc
    10921 tcctagaaaa atatagtgaa gtttataagg ataaagctga cttatatttt tgtttttata
    10981 aaaagattat agatatttta aagcaaggag ggattggcag tgttataact ccaagatatt
    11041 ttttagaaag tctatcaggt aaagatttaa gagaatatat taaatcaaat gtcaatgttc
    11101 aagaaatagt ggatttttta ggtgcaaata tatttaaaaa tataggggta tctagttgta
    11161 ttctaacttt tgataagaaa aaaacaaaag agacatatat agatgtattt aaaatcaaaa
    11221 atgaggatat atgtataaat aaatttgaaa cattggaaga acttctaaag agtagcaaat
    11281 ttgaacattt taatataaat cagagattgc tatctgacga atggatactt gtaaataaag
    11341 atgatgaaac tttttataat aagatacaag aaaaatgtaa atatagcttg gaagatatag
    11401 caataagttt tcaaggaata attacagggt gtgataaagc gtttatttta agtaaagatg
    11461 atgtaaagtt gaaccttgta gatgataaat ttttaaaatg ttggataaaa agtaagaata
    11521 taaataaata tatagtagat aaaagtgaat ataggctaat atactcaaat gatatagata
    11581 atgagaatac aaataaaagg atactagatg aaattatagg gctatataaa acaaaattag
    11641 aaaacagaag agaatgtaaa agtggaataa ggaaatggta tgaacttcaa tggggtagag
    11701 aaaagctttt ctttgaaaga aaaaagataa tgtatcctta taaatcaaat gaaaatagat
    11761 ttgctataga ttatgataat aatttttcaa gtgcagatgt atattctttt tttataaaag
    11821 aagagtattt agataaattt tcatatgaat atctagttgg aattttaaat tccagtgttt
    11881 atgataaata ctttaaaata actgctaaaa aaatgagtaa aaatatatat gattactatc
    11941 ctaataaggt tatgaagata aggattttta gagataataa ttatgaagag atagagaatt
    12001 tatcaaagca aataatatca attttattaa ataagtctat agataaagga aaagtagaaa
    12061 aattgcagat aaaaatggat aacttgataa tggattcttt aggtatataa gcagttgtaa
    12121 gtattaaa
//
LOCUS       DAMJYR010000114        12114 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.117, whole genome shotgun sequence.
ACCESSION   DAMJYR010000114 DAMJYR010000000
VERSION     DAMJYR010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12114)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12114)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12114
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.117"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            63..938
                     /locus_tag="PC471_002961"
     CDS             63..938
                     /locus_tag="PC471_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase"
                     /protein_id="HDF2790071.1"
                     /translation="MITFDVNGAIIRFDDTKNNYNKIRKIFKLYALEISKNFERDCLN
                     SFQNLKQISDRGLSLGEKYIEESFKKGIETIVSFGIITIDINTFKSVYCEKYLDFKRL
                     FNNLNKTFIIPNKNKKNGHASFLEIESTIKKMSNYLYNDCFKIHYAVVDALIENRITA
                     VHSYIDEESIKKSNALFNNYKDGFIGKADECKVVKQIITLNPYREDVYEFLVKEDGDF
                     SKEIERLTEFLGYDIREYKASLMDIYINELMESDENDVENAKEKIEKYARYIGCSDEN
                     LYVARVNAIYTFANA"
     gene            1297..2601
                     /locus_tag="PC471_002962"
     CDS             1297..2601
                     /locus_tag="PC471_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase/BAAT N-terminal
                     domain-containing protein"
                     /protein_id="HDF2790072.1"
                     /translation="MKVNVYNVESFVDERLKIMVSDLIPNTKLKISVKMEFPWCKGEE
                     FSSYGVFCSNEKGEVDLDLVEPIEGTYKADNCMGLIYSLEKSKTEGKNFAENISIDKP
                     IIMNMIFETSIERKEVELKRIFKSEDITTKYISDEFIGKLFYKQNSNNKTILMLGGSD
                     GNLDALDLLAAPLASRGFNVLTVAYFALEGLPDKLEEVPLEYFEKVFEWISENEITST
                     KEIFVHGTSKGGELAFLLASRYEQIKKVVVSQPHIYCFQALNGLMSGNDTSSWSYKGE
                     PFPYIKVDNDIFFEEQKKNISKGIPFGFNSTYKKSLKRAENRKEARIKIENSKADILM
                     IAGKEDNIWNSYEACLKALEIFEENNYPYNVELLTYNNMGHPLPIPYIMPLKETLCMS
                     MSGGIFSSGGTVEGNSKGQFESWNRTIEFYKKPLSSDLNNYY"
     gene            2785..4176
                     /locus_tag="PC471_002963"
     CDS             2785..4176
                     /locus_tag="PC471_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="benzoate/H(+) symporter BenE family transporter"
                     /protein_id="HDF2790073.1"
                     /translation="MERLARREQPHIKFGMFKIRIPFIHYRFEKPEAIQGIISSMTSL
                     GTIGLSTQILGLPYEVAWSMAIINSILYCLHVFMGDPVVPGWITASITLTTAYLLKFS
                     MGIERIQALTALQIDLGIIFILMGITGVAGKLVSKIPNSIKGGILMGVGISTIISEFN
                     PKGRFDSYPISITVGILVACFVMFSERFDTLKVKNKFLFHLGEYGVVSAILVSLIVGI
                     FSKEIEIPRFSFDNLVYIVDFKNLINTVSPFGIGFPSVMLFIQGIPMAFMIYIIAFGD
                     FITGENLVLSESENRKDEYIDFNSNRSNVISGIRNIFMAIFSPYIPMCGPLAATLTGS
                     VAQRYKVGKEAMQSVFSGMGTLVWVSAIFICFYPIAQIATPLIPLALSVTLLVQGYLC
                     TKLSMELCNTGVEKGLIGLMGGVIAAKGGTWGLAVGFILYFILIDSKKRKEKDINSIE
                     DCVYEILDKKKSI"
     gene            4353..4940
                     /locus_tag="PC471_002964"
     CDS             4353..4940
                     /locus_tag="PC471_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDF2790074.1"
                     /translation="MPPKVKITKEMILNTVLDITREVGFEAVNARSIASKLQCSTRPI
                     FTCYENMNELKNEFLAFAYEYYNQYVVNYRNSKSVSPYLLLPLSYIEFAQEETYLFKL
                     LFINDLDLMMKEANDFYKEIDNEKKARLFSESIGIDLECAKVIFLDLFLYTHGIAVLT
                     ATKKLTLDRYSAEKMLKNILTAFIRQEKSDWNLSI"
     gene            4973..5764
                     /locus_tag="PC471_002965"
     CDS             4973..5764
                     /locus_tag="PC471_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDF2790075.1"
                     /translation="METKQYLIDFYNTYDEDSRLALKHGMVEFLTTMHYIDKYIKSGD
                     CVLEIGAATGRYSHTLARQGYDVDAVELVEHNIEVFHKNTQSNENISITQGNAMDLSV
                     FPDNKYDITLLLGPLYHLYNKEDKQQALHEAIRVTKPGGVVFAAYVISDGCLIDEGFH
                     RGNINVSEYIEKGLIDPQTFAAKSEPKDLFELVRKENIDDLMSAFNVTRLHYVASDGL
                     ALYMREAVDSMDDDAFALYLKYHLATCEREDLVGVTSHAIDIFRK"
     gene            complement(5945..8362)
                     /locus_tag="PC471_002966"
     CDS             complement(5945..8362)
                     /locus_tag="PC471_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2790076.1"
                     /translation="MFRNNNRTFIRKIALNDLKINKLKTYLSGIIIMISTCLLLTVTL
                     VSYNASVDLVNASPYHAIYKSVDEKAKNILYKDKNFSGVGTYKLIGSNKKTDCIMSIV
                     YADDTAIKLMNFQPLKGKLPTNKNEIAISEKYLQEFGLDKDIGDSIKLNYYDDITNKE
                     VQCDFIIVGFLENYYQDNAKQYYTVVSNDYFKNIASTPLQNKSNTFDESRPDTVDVLV
                     KLSEENTEKDSASIKTQLNKIGLSLGIKKYNIYLNDNYIESNLIDGEQIITVLVVGIV
                     VLFSSVFVIYSIFYISVVNSVQMYAKLKSLGMTSFQLKKIISLQGNILSIIFIPLGVI
                     ASCIIAYIIQPLAWQMIADLFIILILSLVMFLTVRISLFKPARIISKISAIEAMQYTE
                     TKFRKKAKNFSYINIKNLALKNIESNRKKNLIALISLSISGVLFISIANLANSIDFKK
                     QLSQQFVYNEDYIISIKSDNLYEHITEIQQNNPLTDSLKNEIKSIHGVKKLIESKSIK
                     SNIIEPYIKDRDEKQFITLIKGITPELSKSLEEYIVSGKIDYTHLDSDSLIINKYRTK
                     YYGLNLKVGDTVTFNIFSGNDIIKKKMKIIGIIDNSNTGGMFFASDKTLESLTPYNTN
                     LDFSILIDKKHSKEVEEKLLSIIQKNSNLMLYSYEDDYRMITRAFQYIIIASYIFVGL
                     ISCFGILNMTNTLINSVLIRKKEFALLQAVGMTRKQLRNMLYREGLNISIKAICTSSI
                     LGYFGSNLLCTFIKDVIRLDFINFKFSIFTILIFSFVLIGIQVLVTELLVRNIEKKSL
                     TERLRSQ"
     gene            complement(8375..9040)
                     /locus_tag="PC471_002967"
     CDS             complement(8375..9040)
                     /locus_tag="PC471_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2790077.1"
                     /translation="MSILQVINLKKYYGQSSNITKALDGVTLSIEKGEFISIVGTSGS
                     GKSTLLNMMGGLDNPTSGKVIIKGKELSTMSDEQLTIFRRRNIGFIFQNYNLVPLLNV
                     YENIILPIGLDGETIDKDFINEIINMLSLEEKLYDIPSNLSGGQQQRVAIARALVTKP
                     AIILADEPTGNLDSRTSSDVLSLLKVTSKKFNQTIVMITHNNEIAQLTDRIIRIEDGK
                     IVN"
     gene            complement(9122..10153)
                     /locus_tag="PC471_002968"
     CDS             complement(9122..10153)
                     /locus_tag="PC471_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF2790078.1"
                     /translation="MNFKKFSFSSIYLIIAVGIIFTAILFSISIYLITDDLKLIALLL
                     IYTIVLCGFIALIIFLLHKKIVIFCSRICHTLDSMMNSEDNIEIELEEETILSRINHR
                     LIRMYEVIQENRNSIAIEKADLQELVSDISHQIKTPIANLKMINTTLLNQTFKTEIQK
                     DFLMDMENQLDKLDFLMQSMVKTSRLETGIITLSKKKNSIYETIATALSGILFDAEKK
                     NIEVTVDCDPNLYIIHDKKWTSEAIFNILDNAVKYTSSNGKIRVATECWVMYTKIDII
                     DNGKGISESRQAEIFKRFYREEDVHDIEGIGIGLYLSRKIISLQGGYIKVTSDIGKGS
                     TFSIFLPNK"
     gene            complement(10143..10829)
                     /locus_tag="PC471_002969"
     CDS             complement(10143..10829)
                     /locus_tag="PC471_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2790079.1"
                     /translation="MEKILVVEDDSILNKTLSYNLIEDGYIITSKFTAKSALKSIFEC
                     EFDLIILDINLPDKSGFELCNEIKENYNIPIIFLTANDMECDMIKGYELGALDYITKP
                     FNINIFKQKIKAFLNHLTIKTKQDYYRDGYLEINFSELSANINGNQIIFTPLEYRTLK
                     LLTENPKSILTRKVLLEKLWDIDANFVDEHTLTSVISRIRSKIEKDNLKYIKTVYGMG
                     YMWLGERNEF"
     gene            complement(10987..12051)
                     /locus_tag="PC471_002970"
     CDS             complement(10987..12051)
                     /locus_tag="PC471_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S ferredoxin"
                     /protein_id="HDF2790080.1"
                     /translation="MKRVDERNTMFARANYKKDSTAYNDYYKKNPDKKEIDDSIRNRP
                     NLCSEGTMTYNELNSPMASSAFDFLSDIKSLCEGKVSDTKVDVDAKSMTKKIKGLAKQ
                     YGASVVGITKLKDYHIYTNRGRHEENYGEEINLTHKYAIVFGCEMDKEMINRAPMICE
                     VIETSKCYVDASIVGMILSYYIRNLGYDARNHMDANYLVMPVFIARDAGLGDIGRNAI
                     LTNKDYGSRLRLGVVTTDIPLLEDEYVDFGLEDFCKVCKKCSFNCPSHSLSNDIKIGD
                     DGKYNWVIEHETCYIKWRYLGTDCGMCISACPFSQNLETIKNTTSFKGNNELIQKALD
                     EYTSKFGKRIFIPGNPSWLK"
BASE COUNT         4391 a         1539 c         1730 g         4454 t
ORIGIN      
        1 tgattataaa gtttatatta aatttatttt attgatgaaa ctaatgggga ggttaaaact
       61 caatgattac ttttgatgta aatggagcta ttatcagatt tgatgataca aaaaataatt
      121 ataataagat aaggaaaatc tttaaattat atgctttgga aatatctaaa aactttgaga
      181 gagattgttt aaatagtttt caaaatctaa agcaaatatc tgatagaggt ttaagtttag
      241 gagaaaaata tatagaagag tcttttaaga aaggaattga gactatagta agttttggaa
      301 ttataactat agatatcaat acctttaaaa gtgtttattg tgaaaaatat ttagatttta
      361 agagactttt taataacttg aataaaactt ttattatacc aaataaaaat aaaaaaaatg
      421 gccacgcgag cttcttagaa atagaatcga caataaagaa aatgagcaat tatttataca
      481 atgattgttt taaaattcat tatgctgttg tagatgccct tatagaaaat cgtataactg
      541 cggtacattc ttatattgat gaagaatcta taaaaaaatc taatgcctta tttaataatt
      601 ataaagatgg ttttattggg aaggctgacg aatgcaaagt agtaaaacaa ataataactt
      661 taaatccata tagggaagat gtatacgaat ttttggtaaa agaagatgga gattttagta
      721 aggaaattga gagattgact gaatttttag gctatgatat aagagaatat aaagcgtcat
      781 taatggatat ttatataaat gagttaatgg agagcgatga aaatgatgtt gaaaatgcaa
      841 aagaaaagat tgaaaaatat gctagatata ttggatgtag tgatgagaat ttatatgtag
      901 caagagttaa tgccatatat acttttgcaa atgcataatt ttattaaaca ttaaatatta
      961 gatgttcgat tatataaatt taaataaaaa aatatcctta ttaataacaa atagtttata
     1021 ttaaaaacca ttcttaaaat gagtggtttt ttattttgaa tatatatatt ctaaacctaa
     1081 tagcattcct atctttaatt agccaaaaat caactggatt tcatatagac tacataattt
     1141 taaattgcta aaaaagataa tatgttgaca aaaatagaat atgttgataa aatgaaatag
     1201 tcaatatggt gtcttaattt aaaaatttat ttatgttgta ttagataaag ctgttttata
     1261 taaaacataa ataattatta taaggagaaa tagattatga aagtaaatgt atacaatgta
     1321 gaatcatttg tggatgaaag acttaagatt atggtttcgg acttaattcc aaatactaaa
     1381 cttaaaataa gtgtgaaaat ggaatttcca tggtgcaagg gagaagaatt ttcatcttat
     1441 ggagtgtttt gttcaaatga aaaaggagaa gtagatttag atttagttga acctatagaa
     1501 ggtacttata aggctgataa ttgtatgggg ttaatttatt cgctggaaaa atctaaaact
     1561 gaaggtaaaa attttgcaga aaatatatct attgataaac caataattat gaatatgata
     1621 tttgaaactt ctattgaaag aaaagaagtt gaattaaaga gaatatttaa atcagaagat
     1681 attaccacaa aatatatatc tgatgagttt ataggaaaat tattttataa acaaaattct
     1741 aataataaaa caattttaat gctaggtggt tctgatggaa atttagacgc tctagacctt
     1801 ttagcagctc cacttgcatc tagaggattc aatgtactaa cagttgcata ctttgcttta
     1861 gaagggttac cagataaatt agaagaggtt ccattagaat attttgaaaa agtatttgag
     1921 tggattagtg aaaatgaaat taccagtact aaagaaatat ttgtacatgg gacatcaaag
     1981 ggtggagaat tggccttttt acttgcttca agatatgaac aaattaaaaa ggttgtagta
     2041 tcacaacctc acatttattg tttccaagct cttaatggat tgatgagtgg aaatgatact
     2101 tcatcatggt catataaagg agagccattt ccatatatca aagtagacaa tgatatattc
     2161 tttgaagaac agaaaaaaaa tatatcaaaa ggtattccat ttggatttaa tagtacttat
     2221 aagaaaagtc ttaaaagagc agagaataga aaagaggcta gaataaaaat agaaaattcc
     2281 aaagcagata tacttatgat tgctggaaaa gaagataaca tatggaatag ttatgaagct
     2341 tgtttaaagg cattagaaat ctttgaagaa aataattatc catataacgt agaattatta
     2401 acatacaata atatgggtca tccgttacca attccatata ttatgcctct aaaggagact
     2461 ttatgtatga gtatgagtgg agggattttt tcttcaggtg gaactgtaga aggaaattca
     2521 aaaggacagt ttgaatcatg gaatagaact attgaatttt ataaaaaacc tttatcaagt
     2581 gatttaaata actactatta agtgatttaa gtaactaaaa atgaagcggt ctttctatat
     2641 atgcacaaaa tctatcaatt ttatagattt aatttattgt ttatataatt tgcatgttat
     2701 atactaattt taatgacatt gataatttat aaacaattat aaagttgtaa acttataaga
     2761 gttattgaaa ggtggagtga agttatggaa agactcgcta gaagggaaca acctcatata
     2821 aaatttggaa tgtttaagat aagaattcca tttatacatt acagatttga gaaaccagaa
     2881 gctattcaag gtataatatc aagtatgaca agtttaggca caattggcct tagtacacaa
     2941 attttgggat taccctatga agtggcatgg agtatggcta tcattaactc aatattatac
     3001 tgtttgcatg tttttatggg agacccagtt gtacctggct ggattacagc ttctataact
     3061 cttacaactg catatttgtt aaagttcagc atgggcattg aaagaataca agcattaaca
     3121 gctttacaaa tagatttagg aataatattt attttaatgg gtataactgg tgtagctgga
     3181 aagttagtta gtaagatacc aaattctatt aaaggtggaa tactaatggg tgtcggtata
     3241 tcgactataa tatcagagtt taatcctaaa gggagatttg attcttatcc tatatctata
     3301 acagtaggaa ttttggttgc ttgttttgta atgttttctg aaagatttga tactctaaaa
     3361 gttaagaata aatttttatt tcatttagga gaatatggtg tagtatcagc tattttagtg
     3421 agtttaatag taggtatatt tagtaaagaa atagaaattc ctagatttag ttttgacaat
     3481 ttagtttaca tagtagattt taaaaatctg ataaatacag tatctccttt tggaataggt
     3541 tttccaagtg taatgttatt tatacaaggg ataccaatgg cttttatgat atacataatt
     3601 gcctttggtg attttataac aggtgaaaac ttagtacttt ctgaaagtga aaatagaaaa
     3661 gatgaatata tagatttcaa ttcaaatagg tctaatgtta taagtgggat aagaaatatt
     3721 tttatggcaa tcttttctcc atatatacca atgtgcggtc cattagctgc gacacttaca
     3781 ggaagtgttg ctcaaaggta caaggttgga aaggaagcca tgcagtctgt ttttagtggc
     3841 atgggtacat tagtatgggt ttcagcaatt tttatctgtt tttatccaat tgcgcagatt
     3901 gctacacctc ttataccatt agcattgtca gtaacacttt tagttcaagg atatttatgt
     3961 acaaagctat caatggagct atgtaataca ggtgttgaga agggattgat tggacttatg
     4021 ggaggtgtaa ttgctgctaa aggcggaaca tggggacttg ctgtaggttt tatactgtat
     4081 ttcattttaa ttgattctaa aaaaagaaaa gaaaaagata ttaattcaat agaagattgt
     4141 gtatatgaaa tattagacaa aaagaagagt atttaaagga aaatctgagt tgctgttatc
     4201 tattaataga ttaattgcaa ctcatgtttt atttataagt tttaggaatt gcaaaaaaat
     4261 gcaaagcttt ttcttgacat aatttcttga tatgttacta tagttatata acacatgtgt
     4321 tttataaaaa tttaatggag aggtgaacat atatgccacc taaagtaaag ataacaaaag
     4381 aaatgatttt gaatacagtc ttggatatta caagagaagt gggatttgaa gctgtaaatg
     4441 cgaggagtat tgcaagtaaa ttacagtgtt ccactcgacc aatttttact tgttatgaaa
     4501 acatgaatga attaaaaaat gaatttcttg cttttgcata tgaatattat aatcagtatg
     4561 ttgttaatta tcgtaattct aaaagtgtaa gcccttattt actgcttccg ctttcataca
     4621 ttgaatttgc tcaagaagaa acgtatttat ttaagttgtt atttataaat gatttagatt
     4681 tgatgatgaa agaggcgaat gatttttata aagaaattga caatgaaaaa aaggcaagac
     4741 ttttttcgga aagcattgga atagatttgg aatgtgcaaa agtaatattt ttagatttat
     4801 ttctttatac tcatggcata gctgtcttaa cagcaacaaa aaaattaaca ttagatagat
     4861 atagtgctga aaaaatgttg aaaaatatat taacagcttt tataagacaa gaaaaatcag
     4921 attggaattt gtctatttga tgtttattaa ctaatataga agaggtgaaa ttatggaaac
     4981 aaaacaatac ttaattgact tttataatac ttatgatgaa gatagtcgac ttgcattaaa
     5041 acatggaatg gtggaatttc tcactactat gcattacatt gataagtaca taaaatcagg
     5101 tgattgtgtg cttgaaattg gcgctgcaac tggtcgttat tctcacacac tggcacgtca
     5161 gggatatgat gttgacgcag tggaattagt cgaacataat atagaggttt tccataaaaa
     5221 tactcaatca aatgaaaata taagcattac tcagggtaat gctatggatt tatctgtttt
     5281 cccagataat aaatatgata ttacgttact gttaggacca ctatatcatc tttataacaa
     5341 agaagataaa caacaagctt tacatgaggc aattcgtgta acaaaacctg gtggtgtggt
     5401 ttttgctgca tatgttatat ctgatggctg tcttattgac gaggggttcc atagaggaaa
     5461 tattaatgta tctgaatata ttgaaaaagg tttgattgat ccacagacat ttgctgctaa
     5521 gtctgaacca aaggatttgt ttgagcttgt acgtaaagaa aacattgatg atttgatgtc
     5581 tgcatttaat gtaactagac tgcattatgt cgcatcagat ggtttggctt tgtatatgcg
     5641 tgaagcagta gactctatgg atgatgatgc atttgcatta tatttaaaat atcaccttgc
     5701 tacttgtgaa cgtgaagatt tagtaggggt tacaagtcat gcaattgaca tatttagaaa
     5761 ataaggaata tgcttgttta tgtctctggt gatgttgctc ggagatataa gtaagtgtat
     5821 ctgtataaaa taaaaacaag tatagggata gaaaattatg taaagtcaaa attttctatc
     5881 cttttagctt ttaaaaaatt atgttaaaac ttgtataaaa aatggctatt ttttatatac
     5941 attcttactg agaacgtaag cgttctgtaa gtgatttttt ttctatattt cttacaagta
     6001 attcagtcac taaaacttgt ataccaatta atacgaatga aaaaattaaa atagtaaata
     6061 tagaaaattt aaaattaata aagtctaatc taattacatc ttttataaaa gtgcaaagta
     6121 gatttgaacc aaaataacct agtatacttg aagtacatat agctttgata cttatgttta
     6181 gaccttctct ataaagcata tttcgcagtt gctttcttgt cattccaacg gcttgaagta
     6241 atgcaaactc tttttttcta attaaaacgc tgtttattag ggtattggtc atatttagta
     6301 taccaaagca agaaattaaa ccaacaaaaa tgtatgaagc tataattata tattgaaatg
     6361 ctctagtaat cattctataa tcatcttcat atgaataaag cattaaattt gaatttttct
     6421 gaattatact taacaacttt tcttcaactt ctttagaatg ctttttatct attaaaatag
     6481 aaaaatctaa atttgtatta tatggtgtta gagattctaa agttttgtct gaagcaaaaa
     6541 acatacctcc tgtattggaa ttgtcaatga ttcctataat tttcattttc tttttaataa
     6601 tatcatttcc actaaagata ttaaatgtaa ctgtatctcc aacttttaaa tttaatccat
     6661 aatattttgt tctatattta tttataatta aactatctga atctaaatgt gtatagtcta
     6721 tttttccact gacaatatat tcttcaagtg attttgaaag ttcaggcgta atacctttaa
     6781 ttagagtaat aaattgtttt tcgtctctat ctttgatgta aggttctata atattacttt
     6841 taattgattt ggattcaatt aattttttta ctccatgaat agatttaatt tcattcttaa
     6901 gagaatctgt tagtggatta ttttgttgga tttctgtaat atgctcatat aagttatcac
     6961 tcttaatgct aattatataa tcttcgttat agacaaattg ttgagatagt tgttttttga
     7021 aatctattga attagcaaga ttagcgattg aaataaataa aactccacta atgcttagcg
     7081 atattaatgc aatcaaattt ttctttctat ttgactcgat attctttaat gctagatttt
     7141 ttatatttat ataactaaaa ttttttgctt tttttctaaa tttagtttct gtatattgca
     7201 tggcttcaat agcagatatt ttagaaataa ttctagctgg tttaaataaa gaaattctaa
     7261 cagttaaaaa cattaccagt gataaaatta aaattataaa taaatcagct atcatttgcc
     7321 atgctaatgg ttgaattatg taagcaatta tacaagaagc tataacccct aatggaataa
     7381 aaataattga caaaatattt ccttgtagag aaataatttt ttttaactga aaagaagtca
     7441 tacctaacga tttcaactta gcatacatct gaactgaatt aacaactgaa atataaaaaa
     7501 tactatatat aacaaaaacg ctagaaaaaa gaacaactat tccaactact aaaactgtaa
     7561 taatctgctc accatcaatt aaatttgatt caatatagtt atcatttaag tatatattat
     7621 atttcttaat acctaaagat aaaccaattt tatttaactg tgtcttaatt gaagctgaat
     7681 ctttctctgt attttcttct gatagtttaa ctaatacatc tacagtatca ggtcttgact
     7741 cgtcaaaagt attactttta ttttgtaaag gtgtagaagc tatattctta aaataatcat
     7801 ttgaaacgac tgtatagtat tgcttggcat tatcttgata ataattttct agaaatccaa
     7861 caataataaa atcacattga acttctttat tcgtaatatc atcataataa tttaatttaa
     7921 tgctatctcc aatatcttta tctaaaccaa attcctgtaa atatttttct gaaatagcaa
     7981 tttcattttt atttgtaggt agtttgcctt tcaatggttg aaaattcata agtttaattg
     8041 ctgtatcatc tgcataaaca atcgacataa tacaatctgt ctttttatta cttccaatta
     8101 atttgtaagt tcctacgcca ctaaagtttt tatctttata tagtatattt tttgcttttt
     8161 catctacaga tttatatata gcatgatatg gagatgcatt aactaaatct acagaggcat
     8221 tatatgaaac taaggtcact gttaataata aacatgtaga aatcattata atgatacctg
     8281 aaagatatgt tttaagttta ttaattttta aatcatttaa agctattttt cttataaatg
     8341 tgcgattatt atttctaaac atatactaca cctcctagtt aacaattttt ccatcttcaa
     8401 tacgaataat tctatcagtt agttgtgcaa tttcattatt gtgtgtaatc attacaattg
     8461 tttggttgaa ttttttactt gtcactttaa gtaaactcag tacatctgaa ctagttctac
     8521 tatcaaggtt acctgttggt tcatcagcta gtataatagc tggttttgtt acaagtgctc
     8581 tagctatagc aacacgttgc tgttgaccac cagaaagatt acttggtata tcatataatt
     8641 tttcttcaag agaaagcata tttataattt catttataaa atccttatct atggtctctc
     8701 catccaatcc tattggtaag ataatatttt catatacatt aagtaaagga acaagattat
     8761 aattttgaaa tataaaacct atatttctgc gtctaaagat tgtaagctgt tcatcagaca
     8821 tagtagataa ttccttaccc tttataataa cctttccaga tgtaggatta tctaatcctc
     8881 ccatcatatt taataatgtg gatttgccac taccagatgt acctacaata gaaataaatt
     8941 cgcctttttc aatagataga gttacaccat caagagcttt tgtaatgttt gaactttgtc
     9001 cataatattt ctttaaatta attacttgta aaatactcat aataatacct ccttaacatc
     9061 ttgtaaatac agtataaaac caaaatgttg cagaatcatt acaaatgaaa tatttaaaaa
     9121 tttatttatt aggtaagaaa atagaaaaag tcgacccttt tccaatatct gatgtaactt
     9181 taatatagcc accttgaaga gatataattt ttcttgaaag atagagacct attccaatac
     9241 cttctatgtc atggacatct tcttcacgat aaaatctttt aaagatttct gcttggcgac
     9301 tttctgatat tccttttcca ttatcaataa tatctatttt tgtatacata acccaacatt
     9361 ctgtagctac acgtatctta ccattagaac ttgtgtattt tacagcatta tctaatatat
     9421 taaaaatagc ttcagaagtc catttcttgt catgaattat atataaattt gggtcacaat
     9481 caactgtaac ttcaatattc tttttttcag catcaaacaa aattccacta agtgctgtag
     9541 caattgtttc ataaattgaa tttttctttt tagatagtgt gattatacct gtctctagtc
     9601 tggaggtttt taccatagat tgcattaaaa aatctaattt atctaattgg ttttccatat
     9661 ccattaaaaa atccttttga atttctgttt taaatgtttg atttagtaga gtagtattaa
     9721 tcatttttaa atttgcaata ggagttttaa tttgatgaga tatatcagaa actagttctt
     9781 gtaaatcagc tttctctata gcaatactat tgcgattttc ttgaataact tcatacattc
     9841 taattaatct gtgattgata cgtgataaaa tagtttcttc ttctaattca atttcaatat
     9901 tatcttcact attcatcata ctatctaaag tgtgacatat tctagaacaa aatataacta
     9961 tttttttatg aagtaaaaat attattaatg ctataaatcc acaaagtact attgtatata
    10021 ttaataatag agcaattagt tttaaatcat cagttattag ataaattgag atactaaata
    10081 aaatagctgt aaaaattata ccaacagcaa ttattaaata aatagaacta aaagaaaatt
    10141 ttttaaaatt catttctttc acctaaccac atatatccca taccatatac cgttttgata
    10201 tacttaagat tgtctttttc aattttgctt ctaatcctac ttataacaga tgttaaagta
    10261 tgttcatcta caaagttagc atctatatcc cagagttttt ctaaaagtac tttccttgtt
    10321 aaaatacttt taggattttc agtaagcagt tttaaagtac gatattctaa tggtgtaaat
    10381 ataatttgat ttccatttat attggcagat aactcagaaa agttaatttc aagatatcca
    10441 tccctatagt aatcttgttt tgtttttatt gtaagatgat ttaaaaaagc tttaattttt
    10501 tgtttaaata tattgatatt aaatggtttt gttatataat ccaaagctcc aagttcatag
    10561 cctttaatca tatcacattc catatcatta gcagttaaaa aaattattgg tatattgtaa
    10621 ttttccttta tttcattaca taattcaaac ccacttttat ctggtaaatt aatatccaaa
    10681 attattaaat caaattcaca ttcaaaaata gactttagtg cagatttagc tgtaaatttt
    10741 gaagtaatta tatatccatc ttcaattagg ttatatgata gagttttatt taatatagaa
    10801 tcatcttcta ctactaatat tttttccata cagttactcc ttttagttta atattttata
    10861 atgtcaatta tagactaaaa acattgctga attgttataa acgagaaaaa gtatttaaaa
    10921 atttatatat ttaataaaga acctgtccta tgataaattc tagtacagat tctttattaa
    10981 ataaaattat ttcaaccaag atggatttcc aggtataaat attcttttac caaattttga
    11041 agtatactca tctaaagctt tttgaataag ctcattatta cccttaaatg aagtagtatt
    11101 ctttatagtt tcgagatttt ggctaaatgg gcaagcagat atacacatac cacaatctgt
    11161 acctaaatat ctccatttaa tatagcatgt ctcatgttca attacccaat tatattttcc
    11221 atcatcacca attttaatat cattagaaag cgaatgagaa gggcagttaa aagagcattt
    11281 tttacagacc ttacaaaaat cttctagtcc aaaatcaaca tattcatctt ctaaaagagg
    11341 aatatctgta gtaacaacac ctaatctaag tctagagcca taatctttat ttgtaagtat
    11401 agcatttctt cctatatctc ctaaaccagc atcacgagct ataaaaacag gcattactaa
    11461 ataattagca tccatatgat ttctagcatc atatcctaaa tttcttatat agtaacttaa
    11521 aatcatgccg acgatagatg catccacata gcactttgat gtttctataa cttcacatat
    11581 cattggagct ctatttatca tttctttatc catctcacat ccaaatacaa ttgcatactt
    11641 atgagttaga ttaatttctt caccgtaatt ttcttcatgt ctacccctat ttgtataaat
    11701 atgatagtct tttaatttag ttataccaac tacagaagct ccatactgct ttgcaagacc
    11761 cttaattttt tttgtcattg actttgcatc tacatctact ttagtatcag aaacttttcc
    11821 ttcgcataaa gatttgatat cagataaaaa atcgaatgca ctacttgcca ttggagagtt
    11881 taactcattg taagtcattg ttccttcact acataaattt ggtctatttc ttatactatc
    11941 atcaatttct tttttgtcag gatttttctt ataatagtca ttataagcag tactatcttt
    12001 tttgtaattt gctcttgcaa acatagtatt tctctcatca acccttttca attctattcc
    12061 ccctagtaaa aatatattta aaaaatataa ctttattgtt gtataattta atta
//
LOCUS       DAMJYR010000115        11811 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.119, whole genome shotgun sequence.
ACCESSION   DAMJYR010000115 DAMJYR010000000
VERSION     DAMJYR010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11811
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.119"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            169..1209
                     /locus_tag="PC471_002971"
     CDS             169..1209
                     /locus_tag="PC471_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDF2790081.1"
                     /translation="MDKHSDSVMYGGMEVYNIWGTTTVDSIIGESDVMRALKNRICKI
                     GNSDSTVAITGESGTGKELIARAIHLAGNRGNKTFVAINCAAIPEPLLESELFGYTKG
                     AFSGADSKGKIGKFELANGGVVFLDEIGDMPIQLQSKLLRVLQEKKFTRIGSNELIDI
                     DVRIISATNRDLFELVKENKFREDLYYRLNVIPLEVPPLRERGDDIQILINNFIDKYS
                     RKMKKNVYHIEPDVEQMLLKYPWPGNIRELENTIELALNLLEEDGIIHKGIINRKILE
                     YFKLNNINIKLLEESDYEISMEQDILTLEEFERAYIKKVLKFYGNDTKSKKLAAKKLG
                     ISLATLYRKIDV"
     gene            1565..2887
                     /locus_tag="PC471_002972"
     CDS             1565..2887
                     /locus_tag="PC471_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HDF2790082.1"
                     /translation="MFDIDGRPPILKCLPLSMQHILAMIVGTVTVPIVVGGAVGVSPT
                     QITLLVQYALIIAGISTLIQLYPIGNIGSRLPIIFGVGFTYVPTLVAVGGNYGLASIL
                     GAQLIGGVATVLIGIFIKKIRKFFPNIVAGTVVFTIGLSLYPIAINYMAGGVNSPTYA
                     SMQNWAVAAITLVVVIGLSQFAKGYAKLAAIFLGIIVGYCVSLVLGIINFDPIREATW
                     FAIPKPLEFGMEFDMPVIISVVIVSIINAVQAIGDLSATTMGGMDRNITDKELSSGVI
                     GSGICTMIGALFGGLPPSSYSQNVGMVAMNKVISRFVLGIAGIFMLLSGFVPKFGALM
                     TTIPYSVLGGATISIFSMITMTGIKLIITEELSSRNITIVGLAIALGMGITSVPAAQE
                     AFPSFVKLVFGESPIVIATLVAFVLNLIIPNKSLEDEAKERAVMEETN"
     gene            3184..4545
                     /locus_tag="PC471_002973"
     CDS             3184..4545
                     /locus_tag="PC471_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine/pyrimidine permease"
                     /protein_id="HDF2790083.1"
                     /translation="MKTRNTSKYDVDGIPPLKESIPLALQHLLAMIVGNMVPAILIAN
                     VVGLNQGQATMLIQGSMLAAGLATFLQLYPIPLFKGFKLGSRLPVMMGMSYVFLGACL
                     SVAAKDGLAALFGAQIAAGVIVFFVGFGVKKIRHIFTPIVSGTIIACMGLGLFATAIK
                     NLAGGEGTQTFGSPINFTVGVIVAFVIIMINKYGKGLVKNSSILIGILVGYAISLVLG
                     LVDFSAVQGAAIVSLPTPAAFGLEFRPELIVMFTIIYIIGIADMMGACTIATVGAMDR
                     EVTDEELASVVLGNSITSIISSLFAALPTGVFSQNTVIVSMNKVTSRFVIALGALVLL
                     LAGISPALGAIMTTIPSCVVGGATLVVFSSIAMSGFSIMSMDGFTEENNLIAGVSIAT
                     SMGLTTAPQVLDQFPETIGTVLGGSSIVSGAIIAILLQTLFKLKSRKSAENVTSNLEE
                     NIG"
     gene            4618..6012
                     /locus_tag="PC471_002974"
     CDS             4618..6012
                     /locus_tag="PC471_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HDF2790084.1"
                     /translation="MIDILIKNAIIVTVNKDRDVIFDGAIAIQGDRILDIGNTVDIEP
                     KYLDAKKVIDANGKVIFPGFINTHNHLFQVLLKGLGDDMALHEWLNTMMFPSAKFLTE
                     QDTYDAAMLGCMEGLKSGITTMVDYMHTHNRPGLTDGIVKAYKDLGIRGVVGRGCIDL
                     GIHKELIEDVETVEKDLRRVFEKYHNSENGRIKICVAPSSMWAISEEMGKMLWNIVKE
                     YDSYFTVHISETEFARTATKDIHGEIDIKLLEKWGIVGPEVVAVHCVCITDEDMEMLK
                     KYDIKVSHNVASNMYLASGVAPVPEMLKKGINVSLGLDGAASNNAQDMVELMKLTALQ
                     HKVNNCDPLAISAEKVLEMATIEGARTLRMEDEIGSLEIGKKADLVIFNSMLSPKSIP
                     LHNPVSTLVYSASMHNVESVLVDGNVILENGKVTTIESEEQVLLNAQHAAEELCKRAN
                     VTNRMEGHKWNSLY"
     gene            6074..6544
                     /locus_tag="PC471_002975"
     CDS             6074..6544
                     /locus_tag="PC471_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HDF2790085.1"
                     /translation="MEKIKLNINDYMYEIEVEKNETLLHVLREKLNLTGAKQGCNSGS
                     CGACKVLIDGVDMKSCKLLAKNCEGKVIYTIESFANGENLHPIQEAFIEVGAVQCGYC
                     TPGMIITTQALLNRNASPNEDEIREAFKENLCRCTGYVKIIEAVKLSAARLGGR"
     gene            6547..8763
                     /locus_tag="PC471_002976"
     CDS             6547..8763
                     /locus_tag="PC471_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HDF2790086.1"
                     /translation="MEKVIGKSYPLKDAALKVTGQMKYVADMKPQNVLYAKMLLSPVA
                     HAKIKSIDTSEAEKVVGVRAIATYLNTSQRKYNGSLRFYEHKIPENEVIFSDTVRFVG
                     DRVAAVAAETPEIAEKAVKLIKVEYEELPAVFTIQDAIKEDAICIHGDSNIVSQNNIN
                     GGDVEKGFKECDYIFEDEYSTPAIHHSAIETHSVIAHYDYNDKLTVHTPCQNTFGFRI
                     ILSQIFGMSLNKVRVIRPAIGGSFGGKLELTIEPVAAALSKMTRRPVKMVLTRKESMI
                     STRTRHGSFVKLKTGVNKDGEVIAQDIKIYTNTGAYASSALNVIGALSHKVFKVYKIP
                     NIKFTGMPVYTNTPIAGAMRGYGSPQIFMAQQAQFAKIAKEIGMDLVDFQNKNAVEPD
                     DVDIIFHGSLGNPRVLDCIEQGKKMFKWDEKKKLPKEEGKYLRGIGMAIGAHGNGVFG
                     AHRDVVTLTLKLNEDGTLTLLTGAHDMGNGVVTMQTMMVAEVLGITPDKVDTFETDTD
                     ACTFNLGDYASRGVFVEGGGAKKTAEKLKNLILEEAEKLLETSKEDLYLDNGCVISKL
                     DENVKASLSDVAVYSQSKSLRELTVVEDYSSPAGRTSYGVHFAEVLVDKETKDIKVVD
                     FVAVHDVGRVINPLNLEGQLEGGIHMGLGYALSEELAFDEQGRPKATDFKTYKLFRTV
                     DMPKCQVAFVDGYEKAGPFGGKSIGECAVVPVAPAVANAVYNATDVEIHSLPIKLK"
     gene            8846..9517
                     /locus_tag="PC471_002977"
     CDS             8846..9517
                     /locus_tag="PC471_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HDF2790087.1"
                     /translation="MAKILGICGSPRKGATEYAVLEALKEAETIPGIETEYWSVRGKK
                     ISPCVHCDACIRKETMCIIKDDIQELEQKILEADGIIVGSPVYDMNITAQLTAVFNRL
                     RPMYLVHPGKLQNKVGAAITTGGTRYGGQELTKLPIINFFLMHEMLVSGGLGGCYIGG
                     TIWSKDGKAKGAQEDETGMDTVKRLGKGAAEAVMVSKFGLEKWNVVKEELDVKKDEKS
                     PLRDH"
     gene            9880..11094
                     /gene="dpaL"
                     /locus_tag="PC471_002978"
     CDS             9880..11094
                     /gene="dpaL"
                     /locus_tag="PC471_002978"
                     /EC_number="4.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopropionate ammonia-lyase"
                     /protein_id="HDF2790088.1"
                     /translation="MLKEIKWKVNNLPKGDKENCIKFLNEEEITKVRNFHKSFPQYKE
                     TPLANLEGLAKKLGVAGVYVKDESYRFGLNAFKVLGGSYSMGRYLAQRLDTDISELGY
                     DKLTSKEIKEKLGEITFFTATDGNHGRGVAWTANKLGQKSVVLMPKGSSEFRLNKIKG
                     EGADASITDLNYDDAVRLANDYAEADDHGVMVQDTAWDGYEEIPAWIMQGYGTMAQEA
                     IEQLKEYGVDRPTHVFVQAGVGSLAGAVQGYVASIYDECPITVVVEADEADCYYKSAE
                     AGDGKPRFVGGDMPTIMAGLACGEPNTIGFEVLKNHAAAFVSAPDWVSAKGMRTLGNP
                     LNGDEKVISGESGAVTTGLLVAAMEREDLADLRKDLKLDENSRILLISTEGDTDPDKY
                     RSIVWDGEYPSI"
     gene            11221..>11811
                     /locus_tag="PC471_002979"
     CDS             11221..>11811
                     /locus_tag="PC471_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HDF2790089.1"
                     /translation="MLNEQRKQEVTEICQKLIQNPSSSGNEEGVVKAIEESFEKLGFD
                     SWSRDRYGNIVGCIKGNKPGKKILFDGHIDTVPVPDASKWSVPPFEGKIVDGKIYGRG
                     TSDMKGQYTAMMSAVAYFAKDTNRDFAGELYVAGVVHEEIFEGVSAREISKAVQPDYV
                     VIGESSELNLKIGQRGRGEIVVETFGKPAHSANPEKG"
BASE COUNT         4467 a         1356 c         2397 g         3591 t
ORIGIN      
        1 aatgaagaaa gtatatctat caaattaaaa ctaagtagat atacttttta ttaagttttg
       61 ttttaaaata atattattaa attataacta agcttttctg ttatgatata attaaataga
      121 taatgtgaat gattattata tagaatactt atttggagtt gatattttat ggacaaacac
      181 agtgattcag taatgtatgg tggaatggaa gtatataata tttggggaac aacaacagta
      241 gactccataa ttggagagtc tgatgtaatg cgtgctctta agaatagaat ttgtaaaatt
      301 ggaaatagtg attctactgt agctataact ggagaaagtg gcactggaaa agaattaata
      361 gcaagagcca ttcatctcgc aggaaacaga gggaataaaa cattcgttgc tataaactgt
      421 gctgctatac cagaaccttt actagaaagt gaactttttg gatatacaaa aggagctttt
      481 agtggagcag attcaaaagg taaaattggt aagtttgaat tggccaatgg aggggttgta
      541 tttttagatg agataggaga tatgccaatt caactacaat caaagttatt gagggtatta
      601 caggaaaaga agtttacgag aataggttca aatgaactaa ttgatataga tgttagaatc
      661 atatcagcta ctaatagaga tttatttgag ttggttaagg aaaataaatt tagagaagac
      721 ttatactata gattgaatgt aataccttta gaagtgcctc cacttagaga aagaggagac
      781 gatatacaaa tattaataaa caattttata gacaaatatt cgagaaagat gaagaaaaac
      841 gtgtaccata tagagccaga tgtggaacaa atgttgctta agtatccttg gccaggaaat
      901 attagagagt tggaaaatac tatagaactg gcattaaacc ttcttgagga agatggaata
      961 atacataaag gaataataaa tagaaagata cttgaatatt ttaagttaaa taatataaat
     1021 ataaagttac ttgaggaaag tgactatgag ataagtatgg aacaagacat acttacactt
     1081 gaagagtttg aaagagccta tataaagaaa gtgttaaagt tttacggaaa tgatactaag
     1141 tctaaaaaac tagctgccaa aaaacttgga atatcactag ctacattgta cagaaaaata
     1201 gatgtataat aattaggctt tttattctca attctaaaat ttgaaattat aaaattgata
     1261 aaaaacactt ggaaaactat tttctaagtg tttttttgtg taatagcatt ttattatcca
     1321 aaaataaaaa aaatttaaaa gaatttaaaa aaataaatgc tttaataaat tatcaaatac
     1381 taacaaatat aataaaaatt atatatgtaa aacaattatg ttaaaaaatt ggcattataa
     1441 ttgcttcata ttaagttgag atgacaaaat aaaaaaagaa aggatgttgt gtgagataat
     1501 aataactgtc attaaaaaat agttgggagt gataaccttg agtaaatatc aaaagaatgt
     1561 ttctatgttt gatattgatg gaaggccacc aattttaaag tgtttgccac tatcaatgca
     1621 acatatacta gcaatgatag ttggaactgt tacagtacct atagtcgtag gaggagctgt
     1681 gggtgttagc cctacacaaa taacactttt agtgcaatat gcattaataa tagctggtat
     1741 atcaacactt atacaactat atccaatagg aaatattggt tcaagattgc ctataatttt
     1801 tggagttggt tttacttatg taccaacact tgtagcagtc ggaggaaatt atggattagc
     1861 aagcattttg ggtgcacaat taataggggg agtggctaca gtattaatag ggatattcat
     1921 aaaaaagata agaaaatttt ttccgaatat agttgcagga acagtggttt ttacaattgg
     1981 attatctttg tatcctatag caataaatta tatggcaggt ggtgtaaata gtcctactta
     2041 tgcctctatg caaaactggg cagtagcggc tataacatta gtggtagtta taggtcttag
     2101 tcaatttgca aaaggatatg caaaattggc agcaatattt ttgggaatta tagttggata
     2161 ttgtgtatct ttagttctgg gtataataaa ttttgaccct attagagagg caacttggtt
     2221 tgcgatacct aaaccgcttg aatttgggat ggaatttgat atgcctgtaa taatctcagt
     2281 agtaattgta agtataatca atgctgttca agcaattgga gatttatcag ctactacaat
     2341 gggaggcatg gataggaata tcacagataa ggaattatca agtggagtaa taggaagtgg
     2401 aatttgtact atgataggag ctttgtttgg aggtttacca ccatcatcat atagccaaaa
     2461 cgtggggatg gtagctatga acaaagtaat aagtagattt gtgcttggaa tagctggtat
     2521 atttatgtta ttgtcggggt ttgtgcctaa atttggtgca ctaatgacta ctataccata
     2581 ttctgtttta ggaggagcta caatatctat atttagtatg ataacaatga caggtataaa
     2641 gttaattatt acagaagagt tgtcatcaag aaatattacc attgttggtt tggcaattgc
     2701 cttaggcatg ggaataacat cagtaccagc agcccaagag gcatttcctt catttgtaaa
     2761 gttagtattt ggagaatctc caatagtaat tgcaacattg gttgcattcg ttttaaatct
     2821 aataatacca aataaaagtc tagaggatga ggctaaagaa agagctgtta tggaagagac
     2881 taattaagat ataataattt aaaagaattg tttaaattat ttagtatgag attgttagat
     2941 tatttttgat agctattcat tttaataaaa aatttaacaa ataaatatat accaggcaat
     3001 taatgttcat ttaagttgaa aatcggggag ggaatattta gaaatttgtt aattttttat
     3061 aaaaatagct cagtttagct gtgtttatcg gttgagcaat aaattcatgg tcataaaaat
     3121 atttatagaa aataattata aaaatattta gtcaagatat ttatgtatgg gaggatttat
     3181 tttatgaaaa cacgtaatac ttcaaaatat gatgtcgatg ggataccacc actaaaggaa
     3241 tcaatacctt tggccttaca acatctgtta gcaatgatag taggaaatat ggtacctgcg
     3301 atattaatag caaacgtggt aggacttaac caggggcaag caacaatgtt gattcagggt
     3361 tctatgttag cagcaggttt agcaacgttt ttacagttat atccaatacc tttatttaaa
     3421 ggatttaagt taggttcaag attacctgta atgatgggaa tgagttatgt atttttagga
     3481 gcttgtcttt cagtagcagc taaagatggt ttagcagctc tatttggagc acagattgct
     3541 gctggggtaa tcgtattctt tgtaggattt ggagttaaga aaattagaca tatattcact
     3601 ccaatagttt ctggaactat aatagcatgt atgggacttg gactattcgc aacagctata
     3661 aagaatttag ctggaggaga aggcacacaa acatttggaa gcccaataaa cttcacagta
     3721 ggagttatag ttgcttttgt aataataatg ataaataaat acggtaaagg tttagtaaaa
     3781 aattcttcaa tattaatagg aatattagta ggttatgcta tatctttagt tttaggattg
     3841 gttgatttct cagccgttca aggagcagct atagtatctt taccaacacc agcagctttt
     3901 ggattagaat ttagaccaga gttaatagta atgtttacta ttatttacat aataggtata
     3961 gctgatatga tgggtgcctg tacaatagct acagtaggag caatggatag agaggtaact
     4021 gacgaagaat tagcatcagt tgttttagga aattctataa cttctataat ttcatcacta
     4081 tttgcagctc ttccaactgg agtatttagc caaaatacag taattgtatc tatgaacaaa
     4141 gttacaagta gatttgtaat agcactaggg gcacttgtat tattactagc agggatatct
     4201 ccagcattgg gtgcaataat gacaactata ccatcttgtg ttgttggtgg agcaacttta
     4261 gtagttttct catcaatagc aatgtctggt ttctctatta tgagtatgga tggattcact
     4321 gaagaaaaca atcttatagc aggtgtatct atagcaacaa gtatgggtct tacaactgct
     4381 ccacaagttt tagaccaatt cccagaaaca attggaactg tccttggagg gtcaagtata
     4441 gtttctggag caataatagc tatattactt caaacattat ttaaattaaa gtcacgtaaa
     4501 agtgctgaaa acgttacttc aaatcttgag gaaaatattg gataaatgta ttagatataa
     4561 tatgagaaaa ccatatggta aaatgttgta atgaaagtat tcaaaggggg atttaagatg
     4621 attgatatac taataaaaaa tgcaataatt gttacagtta ataaagacag agatgttata
     4681 tttgatggag caatagctat tcaaggtgac agaatcttgg acataggaaa tacagttgat
     4741 attgagccta agtatcttga tgctaaaaaa gttatagatg ctaatggaaa agtaatattc
     4801 cctggcttta taaatactca taatcacctg ttccaagtgt tattaaaagg tttaggtgat
     4861 gatatggcac ttcatgagtg gttaaataca atgatgtttc catcagcaaa attcttaaca
     4921 gaacaagata cttatgatgc tgcaatgctt ggatgtatgg aaggtttaaa aagtggtata
     4981 actacaatgg ttgattatat gcatactcac aatagaccag gacttacaga tggaatagta
     5041 aaggcatata aagatttagg tataagaggg gttgtaggta gaggttgtat agatttaggt
     5101 attcacaaag agcttataga agatgtggaa acggttgaaa aagatttgag aagggtattc
     5161 gaaaaatatc ataattcaga aaatggaaga ataaaaatct gtgtagctcc ttcttcaatg
     5221 tgggctatat ctgaagaaat gggtaagatg ctttggaata tagttaaaga atatgattca
     5281 tattttacag ttcatatatc tgaaacagaa tttgcaagaa cagcaacaaa ggatatacat
     5341 ggagaaatag atataaaatt acttgaaaaa tggggtatag taggtccaga agttgtagca
     5401 gtacattgtg tatgtataac tgatgaagac atggaaatgc ttaaaaagta tgacataaaa
     5461 gtttctcata atgtagcaag taacatgtat cttgcttctg gtgttgcacc tgtgccagaa
     5521 atgttaaaaa aaggtataaa tgtgagttta ggtcttgacg gtgcagcaag taataacgca
     5581 caagatatgg tagagcttat gaaattaaca gctttacaac acaaagtaaa taattgtgac
     5641 cctctggcaa tttctgcaga gaaagttctt gaaatggcta caattgaggg agcaagaact
     5701 cttagaatgg aagatgaaat aggttctttg gagattggga aaaaagctga cctagtaata
     5761 tttaactcaa tgctttctcc aaaatcaata cctcttcaca atcctgtatc aactttggta
     5821 tactcagcta gtatgcacaa tgtagagagt gttttagttg atggaaatgt aatacttgaa
     5881 aatggaaaag taactacaat tgaaagtgaa gaacaagtgt tgcttaatgc acaacatgca
     5941 gcagaagaat tatgtaagag agctaatgta actaatagaa tggaaggaca taagtggaat
     6001 agcttatact aatattaaaa taagattgta ataatattct attacaacat tagtttatct
     6061 gtaggaggag aaaatggaaa aaataaaatt aaatattaat gattacatgt atgaaataga
     6121 agttgaaaag aatgaaactc ttttgcatgt acttcgtgaa aaattaaatc taactggagc
     6181 gaaacaaggt tgtaatagtg gcagttgtgg agcatgtaaa gttttgatag atggagttga
     6241 catgaaatct tgtaaacttc ttgcaaaaaa ctgcgaaggt aaagttatat acactataga
     6301 aagttttgct aatggagaaa atcttcatcc aatacaagaa gcttttatag aagtcggagc
     6361 tgtacaatgt ggatattgta ctcctggtat gattataaca acacaagctc ttttaaatag
     6421 aaatgcatct cctaatgaag atgaaattag agaagctttt aaagaaaatc tctgtagatg
     6481 cacaggatat gtaaagatta tagaagctgt aaaattatct gcagctagat tagggggtag
     6541 ataattatgg aaaaggtaat aggaaaatca taccctctaa aagatgcagc tttaaaagtt
     6601 actggtcaaa tgaaatacgt ggctgatatg aaaccacaaa atgtacttta cgctaagatg
     6661 cttttaagcc ctgtagcaca tgcaaaaata aaaagtatag atacaagtga agctgagaaa
     6721 gtagtaggtg ttagagctat agctacttat ctcaatacat cacaaagaaa atacaatggt
     6781 tcattaagat tttatgagca caaaatacct gaaaatgaag ttatattttc agatacagtt
     6841 agatttgttg gtgaccgtgt tgcagcagta gctgcagaga ctccagaaat agcagagaaa
     6901 gctgtaaaac ttataaaagt tgaatatgaa gaattgccag ctgtatttac tatacaagat
     6961 gcaataaaag aagatgctat ctgcatacat ggagattcaa atatagtttc tcaaaacaac
     7021 ataaatgggg gagatgtaga aaaaggcttc aaggaatgtg attatatatt tgaagatgaa
     7081 tactctactc cagcaataca tcatagtgca atagagactc atagtgtaat tgcacactat
     7141 gattataatg ataaattaac tgtacataca ccttgccaaa atacatttgg atttagaata
     7201 atactatctc aaatttttgg gatgtcatta aataaagtaa gagttataag acctgctatt
     7261 ggaggctcat ttggtggaaa attagaactt acaattgaac cagtagcagc cgcattatca
     7321 aaaatgacaa gaagaccagt taaaatggtt cttacaagaa aggaatctat gatatcaaca
     7381 agaactcgtc atgggtcttt tgtaaaatta aaaactggtg taaataaaga tggtgaagta
     7441 atagcacaag acataaaaat atatacaaat acaggagcat atgcatcaag tgctttaaat
     7501 gttataggag cacttagtca taaagtattt aaagtttata aaataccaaa tataaagttt
     7561 actgggatgc ctgtatatac aaatacacca atagcaggag ctatgagagg ctatgggtca
     7621 cctcaaatat ttatggctca acaagctcaa tttgcgaaaa tagcaaagga aataggtatg
     7681 gatttagtcg attttcaaaa taaaaatgca gtagagccag atgatgtcga tataatcttc
     7741 cacggttctc ttggaaatcc tagagtattg gattgtatag agcaaggtaa gaagatgttt
     7801 aagtgggatg aaaagaaaaa acttccaaaa gaagaaggaa aatatttaag aggtataggt
     7861 atggcaatag gtgcacatgg taatggagtt tttggtgcac atagagatgt tgttacattg
     7921 actcttaaat taaatgaaga tggtacgttg acactactaa caggagctca tgatatgggt
     7981 aatggtgttg taactatgca aacaatgatg gtagctgaag ttttaggaat aacacctgat
     8041 aaagttgata catttgaaac tgatacggat gcatgtacat tcaaccttgg tgactatgca
     8101 agtagagggg tatttgtaga aggtggagga gctaaaaaga cagcagagaa attgaaaaat
     8161 cttatattag aagaagcaga aaaattactt gaaacatcaa aagaagattt atatctagac
     8221 aatggatgtg taattagtaa acttgatgaa aatgtaaaag catcactttc tgatgtcgca
     8281 gtatatagtc aaagtaaatc attaagagag ttaactgttg ttgaagatta ttcttcacca
     8341 gctggacgta cttcttatgg tgtacatttt gctgaagttt tagtagataa agaaactaag
     8401 gatataaaag tagttgattt tgtagctgta catgatgttg gtagagttat aaatcctcta
     8461 aatttagaag gacaattaga aggtgggatt catatgggtc taggatacgc attgagtgaa
     8521 gaattggcat ttgatgaaca aggtagacct aaagctactg attttaaaac atataaatta
     8581 tttagaactg tagatatgcc aaaatgtcaa gttgcatttg tagatggtta tgaaaaagct
     8641 gggccttttg gtggtaagag tattggagaa tgtgccgttg taccagttgc gccagctgtt
     8701 gcaaatgcag tatataatgc aactgatgta gagatacatt cattaccaat aaaattaaaa
     8761 tagtagaaat taaagcagaa ataacaattt attgtatttg taaatagata ataaaaatag
     8821 atttatttgg tgaggagata ttattatggc aaagatatta ggtatttgtg gaagtccacg
     8881 taaaggagca acagaatatg cagtgttaga agctttgaaa gaagcagaaa ctatacctgg
     8941 tatagaaact gagtattggt cagttagagg gaaaaaaata agtccttgtg tacattgtga
     9001 tgcatgtata agaaaagaaa ctatgtgtat tataaaagat gatatccaag aattagagca
     9061 aaaaatattg gaggcagatg gaattatagt aggttctcca gtttatgata tgaatataac
     9121 tgctcaactt acagcagtat ttaatagact tcgtccaatg tatttagttc atccaggaaa
     9181 acttcaaaat aaggttggtg cagctataac tactggtgga actagatatg gtggccaaga
     9241 attgacaaaa cttcctataa taaatttctt cctaatgcat gaaatgttag tgtctggagg
     9301 tttaggtgga tgctatatag gtggtacaat atggagtaaa gatggaaaag caaaaggtgc
     9361 ccaagaggat gagacaggta tggacacagt aaaaagactt ggaaaaggtg cagcagaggc
     9421 agttatggtt tctaagtttg gtcttgaaaa atggaatgtc gtaaaagaag aattagatgt
     9481 taaaaaagat gaaaaatcac ctcttagaga ccactaaaga tttattatat tattgaatat
     9541 atacaaatag tgatgtaata gattattgca tcactatttt atgttttaat ttagatttta
     9601 taaattttat attaaaaaat atagaaacaa agtgttttga gatttatttt taaattttaa
     9661 aaatgagaat taaaaagtga gaagatagaa aaggtttttt agttattaaa ttctacatac
     9721 attgtatgaa ctaaagatag aaaagaaaaa ataataaaag cattgcaatt tctatactaa
     9781 agagtcatgg aaaaatatct agtatatatt tagtattggc atgttaattg cttatatatt
     9841 aaagtataaa aatttaacaa taatttggag gtattttaga tgttaaaaga aattaaatgg
     9901 aaagtaaata atttgccaaa gggcgataag gaaaattgta tcaaattttt aaatgaagaa
     9961 gaaataacta aggttagaaa ttttcacaaa agctttcctc aatataaaga aactccatta
    10021 gcaaacctag aaggtttagc aaaaaaatta ggtgttgcag gagtatatgt aaaagatgaa
    10081 tcttatagat ttggattaaa tgcatttaaa gtattaggtg gttcttattc tatgggtaga
    10141 tacttagctc aaagattaga tacagatata tctgagttag ggtatgacaa attgacttcg
    10201 aaagaaataa aagaaaaatt aggagaaata actttcttta cagctacaga tggaaatcac
    10261 ggtagaggag ttgcttggac agctaataaa ttaggtcaaa aatctgttgt attaatgcca
    10321 aaaggttctt ctgaatttag attaaataaa attaaaggag aaggtgcaga tgctagtata
    10381 acagacttaa attatgatga tgcagtaaga ttagctaatg attatgcaga agctgatgac
    10441 catggagtaa tggttcaaga tacagcatgg gacggatatg aagaaatacc agcatggata
    10501 atgcaaggat atggaactat ggctcaagaa gctatagagc aattaaaaga atatggagta
    10561 gatagaccaa cacacgtatt tgttcaagca ggagttggtt cacttgctgg tgcagttcaa
    10621 gggtatgttg cttcaatata tgatgaatgc ccaataacag tagtagtaga ggctgatgaa
    10681 gctgattgtt actataaatc agcagaagct ggagatggaa aaccaagatt tgttggagga
    10741 gatatgccaa ctataatggc agggcttgct tgtggagagc caaatactat aggatttgaa
    10801 gtattaaaaa atcatgcagc agcatttgtt tcagcaccag actgggtttc tgctaaaggt
    10861 atgagaacat taggtaatcc tttaaatggt gatgaaaagg taatttctgg agaatctgga
    10921 gcagttacaa ctggtctttt agtagcagct atggaaagag aagatttagc agatttaaga
    10981 aaagatttaa aattagatga aaattcaaga atacttttaa taagcacaga aggagatact
    11041 gaccctgaca aatatagaag tatagtttgg gatggagaat atccaagtat ataaggaatt
    11101 actaaaatgc aattattaga tattggttta catatttaga agatatataa gttctaaaat
    11161 ttagaaaata tataagtata taaaatacat acattataaa aaatctaggg ggataatgaa
    11221 atgttaaacg aacaaagaaa acaagaagta actgaaatat gccaaaaatt aattcaaaat
    11281 cctagttctt ctggaaatga agaaggtgtt gtaaaagcaa tagaagagtc ttttgaaaaa
    11341 ttaggttttg atagttggtc aagagataga tatggaaata tagtaggatg tataaaaggt
    11401 aacaagccag gtaagaaaat attatttgat ggacatatag atacagttcc agttccagat
    11461 gcaagtaaat ggagtgttcc tccttttgaa ggaaaaatag ttgatggaaa gatatatggt
    11521 agaggaactt ctgacatgaa aggtcaatat actgcaatga tgtctgctgt agcgtatttt
    11581 gctaaagaca ctaatagaga ttttgctgga gaactatatg tagcaggtgt tgttcatgaa
    11641 gaaatatttg aaggtgtttc ggctagagag ataagtaagg ctgttcaacc tgactatgtt
    11701 gtaataggtg agtcttcaga gttaaactta aagattggtc agagaggtag aggagaaata
    11761 gtagtagaaa cttttggtaa accagctcac tctgctaatc cagaaaaagg t
//
LOCUS       DAMJYR010000116        11539 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.120, whole genome shotgun sequence.
ACCESSION   DAMJYR010000116 DAMJYR010000000
VERSION     DAMJYR010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11539)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11539)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11539
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.120"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(224..790)
                     /locus_tag="PC471_002980"
     CDS             complement(224..790)
                     /locus_tag="PC471_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HDF2790090.1"
                     /translation="MDTKKVTFSSMCIVINIVLGMVISMINIPLLFLDTVGTVLGAVV
                     LGPFWGALIGGCTNLVLGVISGPTNIPFALVNIVLGLIVGYISKKKGFGYKEAIITGV
                     ILSIVCPLIGTPISVLLFGGLSGSGADLLVGFLVQSGQKIFTAAFFPRILSNIVDKPL
                     SCIMVVYFISKMPKGFIKQYSKNTSSSY"
     gene            956..1477
                     /locus_tag="PC471_002981"
     CDS             956..1477
                     /locus_tag="PC471_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HDF2790091.1"
                     /translation="MKRNREIVVVCHCILNCNSKVEGLSEFKGAQNIAINLINKGYGI
                     IQLPCPEMIMYGINRWGHVKEQFDNLFFREQCRQMLIPHVRQFDEYLKNDYDIKSIIA
                     IDGSPSCGYNKTCSSKRWFGELSGCNELMDKIKDIQMVDGKGIFIEELEKLLKEYNLE
                     IPIVGFDELREDK"
     gene            2276..4354
                     /locus_tag="PC471_002982"
     CDS             2276..4354
                     /locus_tag="PC471_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HDF2790092.1"
                     /translation="MIKISEDYIEKRFDNELLRIEAWGKNSLRIRSFVDQNFVDENYA
                     LNEKPKLNKDDITINKNEDGSAIIKNGKIKAVLDHRDRITFYNDKNEILLKEYIRLRA
                     VKHDDGGEDVGTIEITKDFNSTLKLKSREYRPSYSGEFEVTTRFESEPSEKIFGMGQY
                     QHKFLDLKNTVLELAQRNSQISVPFYISSLGYGLLWNNPGIGKVSFAKNMTEWKMFST
                     NFIDYWITCGESPKELNKNYSQVTGTVPMMPENLLGLWQSKLRYRTSEEVLDVVKEYS
                     KRGIKLSSIAIDYFHWPKQGEYKFDLDYWKNPKELVKKLKEEYSVEPIVSVWPTVQSD
                     AENYNDYLENGYLVNVNRGVRMTMQIQGNTVFVDMTNENAREYVWDRIDKNYKQLGID
                     YYWLDVAEPGYSVYDFDNYRYKKGNVLSCGNIYPIDYLKMIYDGLHNDIESVVTLVRG
                     AWAGAQKYGALVWSGDIDSSFEAFNNQVNTGLNMGLAGIPWWTTDIGGFHGGNPKDPE
                     FRELMVRWFQYATFSPILRMHGDRLPHSKPLSNKGGGSMVTGAPNEIWSYGEEVEVIL
                     TKFIKIRESLKTYLKKLMKEAHEDGTPVMRTLFYEFPEDDKTWEVDNTYMLGDEILVA
                     PIMNYKDRSRKVYLPKGHTWENIFSGVSYEGGKTYEVECPLEEIPIFLKQDSSYNFKE
                     LKNILGEVKS"
     gene            4354..5133
                     /locus_tag="PC471_002983"
     CDS             4354..5133
                     /locus_tag="PC471_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDF2790093.1"
                     /translation="MEPQLWQLLLIVLYGFFINYEKNSTMFGTYQPVTAGFITGLILG
                     DINTGLYIGGTLQLLSLGISNFGGASIPDYQTASIVATFITITTKQEASVGISIGIPV
                     ALLMVQLDVLRNTIGIWLVHKAEDGAKKGNYKNITYMQMLGVLLTAATTGIPVALSVI
                     FGPSLINTILKYTPEWLTGGLTVAGGLLPAVGIGLLLRYLPAKEYFSYLVIGFVLAVY
                     MKVPLLGVALIGGAIALIIYKKNLENQEQQYTVVGGMDEDE"
     gene            5126..5935
                     /locus_tag="PC471_002984"
     CDS             5126..5935
                     /locus_tag="PC471_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HDF2790094.1"
                     /translation="MSDKVSLNKSNEKVKKSVLWRWFFTSSVSSNYEKMQALAYCYAV
                     LPFLKVVYKDNPEKLQSAVLNHLQFFNTNPWVAPYILGINVAMEELSSEETEEAVASI
                     KTGLMGPVAGLGDSLFVVIPWTIFGAIAANMAIDGSPFGILLWIVVSVALKLLSIPLF
                     NAGYTSGTKLISSIEKSLKILTESTSILGLMVVGALIPSVVKANVALNFQQGDFTMKG
                     QEILDQIMPGLVPAILVVIVYWALKKNIKPIYLILGVMVVSIILYALGILK"
     gene            5955..6437
                     /locus_tag="PC471_002985"
     CDS             5955..6437
                     /locus_tag="PC471_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDF2790095.1"
                     /translation="MEMKGIKNIRIDDRLIHGQVATMWSNKLGVTRLMVVNDAVANNS
                     VQKQVLRMATPAGIASSIITEETAIKNITAGKYEGQNVLLIVKSPVDLIPFVEAGFKI
                     HNINVGNMSSRKDTTVLRPNISVTEEEKEAFKRLLDEGIEITTIMTPDDKKTYLVDIL
                     "
     gene            6458..6862
                     /locus_tag="PC471_002986"
     CDS             6458..6862
                     /locus_tag="PC471_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDF2790096.1"
                     /translation="MNSIILMSHGNMAEEVLNSAKMIIGDSIDYPTVNMKQDDGIEGT
                     IEKLKKALEVYKESSEIIIMADLLGGTPCNAALLVANNDSRIKIISGLNLGMVIESAF
                     LDTKELAAKLEKIGKDNISMVEQNANLLSDDE"
     gene            complement(7018..8178)
                     /locus_tag="PC471_002987"
     CDS             complement(7018..8178)
                     /locus_tag="PC471_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HDF2790097.1"
                     /translation="MVTDKYTIREMNERLVLEQIIKNGPISRASIASTIGLNKATISA
                     ITKKLIDESLVHEIGIGNSTHSGGRKPILLVFNKCAGISLSMDIGYDYIFSSLSYLDG
                     TIINSKKLTDIQVSKDNVIQLIDEIINSYNISKIDTPYKVIGLTLAIHGITCENKVLF
                     TPYYNLNEIDLYSILSKKYDFPIHIENEANLTALAENTFSTVHNSLLSLSIHSGFGSG
                     IIINNKLYSGRNGMSGEIGHTIIMPNGKLCPCGNRGCLEQYCSEKKVFEQLSSLENIP
                     KIDSDIVKQLYYEDNHNAKKVIHEFCSYLTIAINNAITTYAPEIIYLNSQIISDIPEI
                     LQITKDMLVSSFNKGINIEISSLGSEASLYGGSAVNIKSFLNIQNLTLINEI"
     gene            8386..9936
                     /gene="xylB"
                     /locus_tag="PC471_002988"
     CDS             8386..9936
                     /gene="xylB"
                     /locus_tag="PC471_002988"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HDF2790098.1"
                     /translation="MKLEYVLGVDIGTSGTKTVLFDKLGNTIKSCTYEYPLIQEKSGW
                     AEQDANDWWKAVVESIREVVQSSNISSECIKGIGLSGQMHGLVMLDNEGKTLRNSIIW
                     CDQRTVKECEEITDLVGEERLIEITANPALTGFTASKILWVRNNEPDIYKNTNKILLP
                     KDYIRYKLTGEYATEVSDASGMQLLDIRKRDWSDEVLEKLNIDKNLLGKVYESQEITG
                     YVTRDVSSLTGLKEGTIVVGGAGDQAAGAIGNGIVKDGVVSSTIGTSGVVFAYTKEPK
                     IDKEGRVHTFCHAIPNTWHVMGVTQGAGLSLKWFKDNFCQSEVEVSNSLGEDVYEIIN
                     NQVSKVPTGCNGLLYLPYMMGERTPHLDPYARGVFFGLSPIHSKKEIARSIMEGVSYS
                     LKDCMDIIENLNIDVNEVRASGGGGKSKVWRQMQADMFNQDVYTINSSEGPALGVAIL
                     ALVGAGIYENIQKACDAIIKTSTKLEPISENVDTYNIYHRLYKRIYKSLKDDFKLLDE
                     VVNAKINN"
     gene            10032..11369
                     /gene="xylA"
                     /locus_tag="PC471_002989"
     CDS             10032..11369
                     /gene="xylA"
                     /locus_tag="PC471_002989"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HDF2790099.1"
                     /translation="MSEIFKGIGQIKFEGVKSDNELAFRYYNPEQVVGNKTMKEHLRF
                     AMSYWHTLCGEGNDPFGVGTVERPWNNITDPIEIAKIKVDAGFEFMSKMGIEYFCFHD
                     RDIAPEGRDLEETNKILDEIVEYIKVNMEKTGIKLLWGTANMFGNPRFVHGASTTCNA
                     DVYAYAAAQVKKAMEITKYLGGENFVFWGGREGYETLLNTNTELEMDNFARFLQMAVD
                     YAKEIGFTGQFLIEPKPKEPTKHQYDFDTATVLGFLRKYNLDKYFKMNIEANHATLAG
                     HTFQHELNIARINNVLGSIDANQGDLLLGWDTDQFPTNIYDATLAMYEVLKQGGIAPG
                     GFNFDSKVRRASFEVEDLFLAYIAGMDTFAKGLLIAHKLLEDEVFENFTKERYASFSE
                     GIGKDIVEGKVGFKELESYALQMPVIKNKSGRQEMLEAILNRYIYEVDTISNK"
BASE COUNT         4461 a         1321 c         2068 g         3689 t
ORIGIN      
        1 aaaataatga aatatatgca ttttttgcgt aatagaatat taattatatt aaaaataaaa
       61 tagcacctct tgatatagat ttatagctca aatttatatc aagaggttac gtttaattaa
      121 agtaatacaa atactatcta attaaattat taataaaaat gttatataaa aatgagtata
      181 tagcatttta aagagtaata aaccttacat aataaatgat ttattaatat gaactagaag
      241 tatttttact atattgtttt ataaaacctt taggcatttt acttataaaa tataccacca
      301 taatacaaga caaaggtttg tctactatat tactaagaat ccttggaaag aacgcagcag
      361 taaatatctt ctgaccagac tgaactaaaa aaccaaccaa taaatcagca ccacttccac
      421 ttaaaccacc aaacaataaa actgaaatag gagtaccaat taaaggacaa acaatagata
      481 aaattactcc tgtaataata gcttctttat aaccaaaacc ttttttctta gaaatatatc
      541 caacaataag accaaggaca atatttacaa gggcaaaagg tatatttgta ggacctgata
      601 taacacctaa aactaagttt gtacatccac caataagagc accccaaaaa ggacctaata
      661 cgacagcccc aagtacagta ccaactgtat ctaaaaataa taatggtata tttatcatag
      721 atataaccat acctaagaca atattaataa ctatgcacat tgaagaaaat gtgacttttt
      781 tagtatccaa ttataatacc tcctaaaaaa atatatacat ttatcatagt atagattata
      841 aaagattact aattgtatat ttctatatag aatattggaa atatagaaca aataaataat
      901 ataattatca aaatatgtag tatcattaaa attaagaata actaaggaga ttattatgaa
      961 aagaaataga gagattgtag tagtatgtca ttgtatatta aattgtaatt ctaaagtgga
     1021 gggattgtca gaatttaagg gagcacaaaa tatagcgata aatttaataa ataaagggta
     1081 tggaataatt caactaccat gcccagaaat gattatgtat ggaataaata ggtggggaca
     1141 tgtaaaagaa cagtttgaca atttgttttt tagagaacaa tgtaggcaaa tgttaatacc
     1201 tcatgtaagg cagtttgatg aatatttaaa aaatgattat gatataaaaa gtatcattgc
     1261 aatagatggc agtccaagtt gtggttataa taagacatgt agttctaaac gttggtttgg
     1321 tgagttaagt ggttgcaatg aattgatgga taaaataaag gatatacaaa tggtggatgg
     1381 gaaaggtatt tttatagaag agctagaaaa gctacttaaa gaatataatt tagagatacc
     1441 tatagttgga tttgacgagc ttagagaaga taagtagaga tataaatatt tattgtctca
     1501 ttatgaaaca ccattatatt tggcagaaaa atatgactga tggacagtac aggaaccatt
     1561 tttaagtgga gtaattagta cttataaaga tgtatccact agctgaaaga aaatgtagaa
     1621 aggtaataaa tgaattaaga agtagataat atatataaag gagtgtctaa aagtgaactt
     1681 tttagtttat gagacactct ttttttatat taaatcaagt atattaaatc aaatatattt
     1741 taatcatttt agcaatgaaa aataaattta tctatatttt gagacaacat cttttttagt
     1801 tttatttatt tgttgtcgtt tttgacttgg tatacaattg tatattttct taaataattt
     1861 cataaattat ttataattat aaaatccatt gttttgaaca atatctaata tattattatc
     1921 tgtatttata aggtcactat acatattttc aagtccgata ttatctacat aataggcaaa
     1981 tgttattcct gtatgtgttt taaaaaaact agatgtatat aggtatcatc acagatatta
     2041 aaagtataaa taaaagatat taagaacaac tttccagcaa tatattagaa aattttacta
     2101 aaatccggaa agcgattgac aaacaaagtc aaataaagta tattattggt aagttagttt
     2161 aatgtacaaa tgaattatat ataaaaccta atttataaat aacgatacta aacgacatta
     2221 tttaacttaa gttacacaaa gatagaatat tataccattg atttaggagg tagccgtgat
     2281 aaaaatatct gaagactata ttgaaaaaag atttgacaat gaactattgc gaatagaagc
     2341 atggggaaaa aactctttac gtattaggtc gtttgtagac caaaattttg ttgatgaaaa
     2401 ctatgctctt aacgaaaaac ctaagttaaa taaagatgat attacaatta ataaaaatga
     2461 agatggaagt gcaatcatta aaaatggaaa gataaaagct gtcttagacc atagagatag
     2521 aattactttt tataatgata aaaatgaaat attattgaaa gaatatattc gattaagagc
     2581 tgtaaaacat gatgatggtg gagaagatgt agggacaatt gagattacca aagactttaa
     2641 ctctactcta aagttaaaat caagagaata tcgaccaagc tatagtggag aatttgaagt
     2701 aactactaga tttgaatctg agccttctga aaagattttt ggaatgggac aatatcaaca
     2761 taaattttta gatttaaaga atactgtttt ggagttagcc caaagaaatt ctcaaatatc
     2821 agttcctttt tatatttcta gtctaggtta tggactttta tggaataatc cgggaattgg
     2881 aaaagtatct tttgctaaaa atatgacaga atggaagatg ttttctacaa attttataga
     2941 ttattggatt acttgtggag aaagcccaaa agagttaaat aaaaattact ctcaagttac
     3001 tggaactgtt cctatgatgc ctgaaaacct tttaggacta tggcaaagta aactaagata
     3061 tagaacttca gaagaagttt tagatgttgt gaaggaatat tctaaaagag gaattaagct
     3121 ttcatcaatt gcaattgact attttcattg gcctaagcaa ggtgaatata aatttgactt
     3181 agattattgg aaaaatccta aagaattagt taaaaaattg aaagaagagt attcagtaga
     3241 accaatagta tctgtatggc ctacagttca aagtgatgca gaaaactaca atgattattt
     3301 agaaaatggc tatttagtca atgttaatcg tggtgttaga atgacaatgc aaattcaagg
     3361 aaatacagtc tttgtagata tgacaaatga aaatgcaaga gaatatgttt gggatagaat
     3421 agataaaaat tataaacaac ttgggattga ctattattgg ttagatgtag ctgaacctgg
     3481 ctattctgtg tatgattttg ataactatag atataaaaaa ggaaatgttt taagttgtgg
     3541 taatatttat cctattgatt atttaaaaat gatttatgat ggtttacata atgacataga
     3601 gtctgttgta actcttgtaa gaggagcttg ggcaggagct caaaagtatg gagctttagt
     3661 atggtctggt gacattgatt ctagttttga agcttttaat aatcaagtaa atacagggtt
     3721 aaatatggga ctggcaggta taccatggtg gactactgat attggaggtt tccatggagg
     3781 aaatcctaaa gacccagaat ttagagaact tatggtaaga tggttccaat atgctacttt
     3841 ttcaccaatt ttgagaatgc atggagatag attgcctcat agtaaaccat tatcaaataa
     3901 aggtggaggc tcaatggtaa ctggagctcc taatgaaata tggtcttatg gtgaagaagt
     3961 tgaagtaatt cttacaaaat tcataaaaat tagagagagc ttaaaaactt atttaaaaaa
     4021 actgatgaaa gaagcgcatg aagatggaac accagttatg agaactttat tttatgaatt
     4081 tcctgaagat gataaaactt gggaggtgga taatacatat atgcttggag atgaaatttt
     4141 agtagctcca ataatgaatt ataaagatag aagtagaaaa gtatatttac caaaaggaca
     4201 tacatgggaa aatatattta gtggagttag ttatgaaggt gggaaaactt atgaagttga
     4261 atgcccactg gaagaaattc ctattttcct aaagcaagat tccagttata attttaaaga
     4321 actaaaaaat attttgggag aggtgaaatc ataatggagc cacaattatg gcaattatta
     4381 ttaatcgtac tatatggatt ttttataaac tatgaaaaaa attcaacaat gtttggaaca
     4441 tatcaaccag taacagcagg ttttattact ggtcttatct taggagatat aaatacaggg
     4501 ttatatattg gaggtacatt acagctatta tcattaggta ttagtaactt tggtggagca
     4561 tcaatacctg attatcaaac agcaagtata gtagccacat ttataactat tacgactaag
     4621 caagaagcat ctgtaggaat ctctataggt attccagttg ctttattgat ggtacaatta
     4681 gatgttctta gaaatactat tggtatatgg ctagttcata aagcagaaga tggagctaaa
     4741 aaaggaaatt ataaaaatat aacctatatg caaatgttag gagtactatt aacagcagct
     4801 acaacaggta ttccagttgc tctatctgta atatttggac cttctttaat taataccatt
     4861 ttgaaatata cacctgaatg gttaacaggt ggtttaacag tagctggagg actacttcca
     4921 gcagtaggga taggattatt acttagatat cttcctgcta aggagtattt tagttactta
     4981 gtaattggtt ttgttttagc agtatatatg aaagtaccat tattaggtgt agctttaatt
     5041 ggtggagcaa ttgcacttat aatttacaaa aagaatttgg aaaatcaaga acaacaatat
     5101 actgtagtag gaggtatgga cgaagatgag tgataaggta tcattaaata aatcaaatga
     5161 aaaggtgaaa aaaagtgtct tatggcgctg gttttttact agttctgttt cttcaaatta
     5221 tgagaagatg caggccttgg catactgtta tgcagtacta ccttttttaa aagtagtata
     5281 caaagataat ccagaaaagt tacaaagtgc tgtattaaat catttacaat tttttaacac
     5341 taatccttgg gttgcccctt atattttggg tattaatgta gcaatggagg agttatcaag
     5401 tgaagagact gaagaggctg ttgcttcaat taaaacaggc ttgatgggac cagtagcagg
     5461 tttaggagat agtttatttg tcgttattcc gtggacaatt tttggagcta tagcagctaa
     5521 tatggcgata gatggaagtc catttggaat cttactgtgg attgtagtga gtgtagcatt
     5581 gaaattatta agtataccac tatttaatgc tggttataca tcaggtacta agctaatatc
     5641 ttcaattgaa aaaagtttaa agattttaac tgagagtaca tctattttag gtttaatggt
     5701 tgtaggtgca ttaatacctt cagttgtaaa agctaatgtt gctttgaact ttcaacaagg
     5761 tgattttaca atgaaaggtc aagaaatatt agatcaaatt atgcctgggt tagttcctgc
     5821 aattttagtt gtgattgttt attgggctct taaaaagaat ataaaaccta tatacttaat
     5881 tttaggagta atggttgttt caataatttt atatgcatta ggtattttaa aataaggaaa
     5941 ggatgatatt agatatggaa atgaaaggta ttaaaaatat tcgtatagat gaccgtttaa
     6001 tccatggtca agttgcaact atgtggagta ataaattggg tgttacaaga ttaatggtag
     6061 taaatgatgc agtagctaac aattctgttc aaaaacaagt tttaagaatg gctacacctg
     6121 caggtatagc gtcatctata attacagaag agactgctat aaaaaatatt actgcaggaa
     6181 agtatgaagg acaaaatgta ttattaattg ttaagtctcc tgtggacctt attccatttg
     6241 tagaggcagg atttaaaata cataatataa atgtaggaaa tatgtcaagt agaaaagata
     6301 caacagttct aagacctaat atcagtgtta ctgaagaaga gaaagaagca tttaaaagac
     6361 ttttagatga aggaattgaa attacaacta taatgacgcc tgatgataag aaaacttatc
     6421 ttgtagacat cttatagatt taaagaggtg tattaaattg aatagtatta ttttgatgag
     6481 tcatggcaat atggctgaag aagtcttaaa ttctgcaaaa atgataatag gggattctat
     6541 agattatcct actgtaaata tgaaacaaga tgatggaata gaaggaacaa tagaaaaatt
     6601 aaaaaaagct ttagaagtgt acaaggaaag ttcagaaata attataatgg cagatttact
     6661 aggtggaact ccatgcaatg ctgctttatt agtagccaat aatgacagtc gaattaaaat
     6721 tatttcaggg ctaaatttag gtatggttat tgaatcagct tttttagata caaaggaatt
     6781 ggcagcaaaa ttagagaaaa taggtaaaga taatatttct atggttgaac aaaatgcaaa
     6841 tttattatct gatgatgagt aggaagatta tattaatatt taaaacgaaa tacttatgtt
     6901 aatacttaat tgatatacaa tttttaaaaa atatagctaa tcttgcataa actatataat
     6961 aaatctaatt ttagaatgta taatttaaag ggttatggct gtatatagtc ataaccctta
     7021 tatttcatta attaaagtta aattttgtat attcaaaaag ctcttgatat ttacagcaga
     7081 gccaccatat agagatgcct cagaccctaa tgatgaaatt tctatattta ttcctttgtt
     7141 aaaagagcta actaacatat cttttgtaat ctgtaatatc tcagggatat cactaattat
     7201 ttggctattt aaatatatta tttcaggagc ataagttgta atagcattat ttatagctat
     7261 tgttaagtaa ctacaaaact catgaataac ttttttggca ttatggttat cttcataata
     7321 tagttgcttt actatatcag aatctatttt aggaatattt tctaaagaag aaagctgttc
     7381 aaataccttt ttttctgaac aatactgttc taaacagcca cgatttccac aaggacatag
     7441 tttaccgtta ggcatgatga tagtgtgacc aatttctcca ctcataccat ttctgccact
     7501 atataattta ttatttatta taatgcctga accaaatcca ctgtgaatac tgagactaag
     7561 tagtgagtta tgtacagttg aaaaagtatt ctcagctaaa gctgttaagt ttgcttcatt
     7621 ttctatatga ataggaaagt catacttttt gctaaggatg ctatataagt caatctcatt
     7681 aagattgtaa tatggagtaa acaatacttt attttcacaa gtaatcccat gtatagccaa
     7741 agtaagacca attaccttat aaggagtgtc tattttggat atgttataac tatttataat
     7801 ttcatctatc aattgtataa cattgtcttt acttacttgt atatctgtta atttcttaga
     7861 atttataata gttccatcta agtaagatag agaagaaaat atatagtcgt atccaatgtc
     7921 catacttaaa gaaatccctg cacatttatt aaatactaat aatatgggtt ttcttccgcc
     7981 actatgagta ctatttccaa ttcctatttc atgaactaga gattcatcaa taagtttttt
     8041 ggtaatagcg gatatagttg ctttattcaa tcctatagta gaagctatac ttgccctaga
     8101 aataggacca ttttttataa tttgttcaag taccaatctt tcattcattt ctcgaatagt
     8161 gtatttatca gtaaccaatt tgatattcct ccttaaaata atattgtaat actttttaca
     8221 caaaaataaa aggttatttt gcattgacaa agataattaa atattttatt attagttcat
     8281 aagttagttt aatatactaa caaaaataaa gcaagtaaaa tatacctaaa atataaaaaa
     8341 attaggatag gaaaacgata gttatgaagt ggcattcaag gagggatgaa gttggagtat
     8401 gtattaggtg tggatattgg tacatcaggt acgaaaacag ttttatttga taaactagga
     8461 aatactataa aaagttgtac atatgaatat cctcttatac aagagaaatc aggttgggca
     8521 gagcaggatg caaatgattg gtggaaagct gtagtcgaat ctataagaga agtagttcaa
     8581 agttcaaata taagcagtga atgtataaaa ggaattggat tatcagggca gatgcatggt
     8641 ctagttatgc tggacaatga aggtaaaaca ttgagaaact ctataatatg gtgtgaccaa
     8701 cgaacagtaa aagagtgtga ggagataact gatttagtag gtgaagaaag actgattgaa
     8761 ataactgcta atccagcact tacaggattt acagcatcaa agatattatg ggttagaaac
     8821 aatgaaccag atatatataa aaatactaat aaaatacttc ttccaaaaga ttatataaga
     8881 tacaaattga caggtgaata tgctacagag gtatcagatg caagtggtat gcagttgctc
     8941 gatataagaa aaagagattg gagcgatgaa gtactagaaa agttaaatat agataaaaat
     9001 cttttaggta aagtttatga atcacaagag attacaggtt atgtgactag agatgtatca
     9061 agtttgacag ggcttaaaga aggaacaata gtggtaggag gagctggaga ccaagcagca
     9121 ggagctatag gaaatggaat agttaaagat ggagtagttt catctactat aggtacatca
     9181 ggtgtagtat ttgcttatac aaaagagcca aaaattgata aagaaggtag agttcatact
     9241 ttctgtcatg caataccaaa tacatggcat gttatgggag ttactcaagg agctggcttg
     9301 tcattaaaat ggtttaaaga taatttctgt caaagtgaag tggaagtttc aaattcttta
     9361 ggtgaagatg tatatgagat tataaataat caagtaagta aagtaccaac aggatgtaat
     9421 ggattgcttt acttaccata tatgatggga gaaagaactc ctcatcttga cccttatgca
     9481 agaggtgtgt tctttggatt atctccaatc cattctaaaa aagaaattgc aaggtcaata
     9541 atggaaggtg tttcatatag tcttaaagac tgtatggata taatagagaa tcttaatata
     9601 gacgttaatg aagtaagagc ctcaggtgga ggtggaaaaa gtaaagtatg gagacagatg
     9661 caagcagata tgtttaatca agatgtatat actataaatt caagtgaagg accagcactt
     9721 ggagttgcaa ttcttgcatt agtaggagct ggaatatatg aaaatataca aaaagcttgt
     9781 gatgctataa taaaaacttc aactaaatta gaaccaataa gtgaaaatgt agatacatac
     9841 aatatatatc atagattgta taaaagaata tacaaatctt tgaaagatga ctttaagtta
     9901 ttagatgaag tagttaatgc aaaaataaat aattaaatta gcttaaaaag aaaaataaag
     9961 atagaaaata gttttaaatt aagaaaacat actacatata ttatatgtgt aaaatattag
    10021 gaggacatga aatgagtgaa atatttaaag gaataggaca gataaaattt gaaggagtaa
    10081 aatctgacaa tgaattagca tttagatact acaatcctga acaagtggta ggtaataaga
    10141 ctatgaaaga acacttaaga tttgcaatga gctattggca tactttatgt ggagagggta
    10201 atgacccatt tggagtaggc acagtagaga gaccttggaa taatataaca gacccaattg
    10261 agatagcaaa gattaaagtt gatgcaggat ttgaatttat gtctaagatg ggaattgagt
    10321 atttctgttt tcatgacaga gatatagcac cagaaggaag agatttagaa gagacaaata
    10381 agatattaga tgaaatagtt gagtatataa aagttaatat ggaaaagaca ggtataaagt
    10441 tattatgggg aactgccaat atgtttggaa atccaagatt tgttcatggt gcatctacaa
    10501 catgtaatgc agatgtatat gcatatgcag cagctcaggt taaaaaagct atggaaataa
    10561 ctaagtactt aggaggagaa aattttgtat tctggggtgg aagagaaggt tatgaaactt
    10621 tacttaacac taatacagaa cttgaaatgg ataactttgc aagattctta caaatggctg
    10681 tagactatgc taaggaaata ggattcactg gacaattctt aatagagcct aagccaaaag
    10741 agcctactaa acatcaatac gattttgata ctgctactgt tttaggattt ttaagaaagt
    10801 ataatctgga taaatacttc aaaatgaata tagaagcaaa ccatgcaaca cttgcaggac
    10861 atactttcca acatgaatta aatatagcaa gaataaacaa tgttttaggt agtatagatg
    10921 caaatcaagg agacctatta ttaggttggg atacagacca atttccaaca aatatatatg
    10981 atgcaacact tgctatgtat gaagtgttaa agcaaggtgg aatagcacca ggagggttta
    11041 attttgactc taaagtaaga agagcatctt ttgaggtaga agacttattc ctagcttata
    11101 ttgcaggaat ggatacattt gcaaagggat tattaatagc tcataaatta ttagaagatg
    11161 aggtatttga aaactttact aaagaaagat atgcaagttt tagtgaaggt ataggtaagg
    11221 atatagtaga aggtaaggtt ggatttaaag agttagaatc ttatgcactg caaatgccag
    11281 ttataaagaa taagtctgga agacaagaaa tgctagaagc tatacttaac agatatatat
    11341 atgaagtaga tacaatttct aataagtaat tgtgtagtaa ttaaacttgg atgtttgaat
    11401 aaaataacag gatatgttct tgtttttatt ctaaaacgga caaagataag tatttgtaaa
    11461 aattctcata atcatgataa attaaggata tagacttaag tataattagt ttatatcctt
    11521 aaaatttcta taaaaaata
//
LOCUS       DAMJYR010000117        11264 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.121, whole genome shotgun sequence.
ACCESSION   DAMJYR010000117 DAMJYR010000000
VERSION     DAMJYR010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11264)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11264)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11264
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.121"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            207..1382
                     /locus_tag="PC471_002990"
     CDS             207..1382
                     /locus_tag="PC471_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HDF2790100.1"
                     /translation="MNLEKIKKFFGFPLDKKHKDNVEKELCNYLYHTGKIICIIIVIS
                     QIIMMISISAREGGPFVTPRRQWYFYMYVTLFCVSSVFMFVFSYLWNKKKDCCTFYIN
                     LWIIYVTFFCLWGCVITLLDQLGGNDLSVFSYIMLAAAAFSVLKFGESVIVFGGCFLF
                     LNLFLPGIQTIPGNEFSNIVNTFFITSLAIFVSTMLYRNRVFTYYDKLLISQQYEEIS
                     HINNLLNDKVMTDDLTKMNNRRYLEDILQKKLISHANQEPITGMMIDIDYFKQFNDNY
                     GHQKGDYCLQNIAKVILDFTSSENEHAIRYGGEEFFICIFNCNEADAKLKSECLRKII
                     ESCNFERDDVPLKCVTVSIGVYTKKIGKTITLNEFISNCDKALYTAKEQGRNCVAFY"
     gene            complement(1555..2952)
                     /gene="asnS"
                     /locus_tag="PC471_002991"
     CDS             complement(1555..2952)
                     /gene="asnS"
                     /locus_tag="PC471_002991"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HDF2790101.1"
                     /translation="MQKEFITVKELYREKEQYIGKTVKVAGWIRTSRVSKNFGFIELN
                     DGSFFKNMQIVFDEKVENFKEVGKLAISSSILVEGELVSTEGAKQPVEIHAKSIVVEG
                     ESDSSYPLQKKRHTLEYLRTIAHLRPRSNTFSAVFRVRSIAAYAIHKFFQERNFVYAH
                     SPIITGSDCEGAGEMFRITTMDLNDIPKTEDGKIDYTQDFFGKEANLTVSGQLNAEIM
                     ALSFRNVYTFGPTFRAENSYTGRHASEFWMIEPEIVFADLEDNMELAEDMIKYVISYV
                     LENAPEEMEFFNSFIDKGLLERLNKIVNSEFIRITYTKAVELLIESGHEFEYPVEWGC
                     DLQTEHERYITEQIYNCPVFVTDYPKDIKAFYMRMNEDGKTVRAMDLLVPGVGEIVGG
                     SQREEREDVLLDRINEMGLREEDYWWYLELRKFGTATHSGFGLGFERIIMYMTGMSNI
                     RDVIPFPRTPKNAEF"
     misc_binding    complement(3029..3262)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(3431..3625)
                     /locus_tag="PC471_002992"
     CDS             complement(3431..3625)
                     /locus_tag="PC471_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3787 domain-containing protein"
                     /protein_id="HDF2790102.1"
                     /translation="MESKKARALKGKDNKRLKSSLLVGNESTAAWADVEKLKPHSKVS
                     IPSIKGVEEAKDWVDNGSKL"
     gene            complement(3898..5571)
                     /locus_tag="PC471_002993"
     CDS             complement(3898..5571)
                     /locus_tag="PC471_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HDF2790103.1"
                     /translation="MEKSYCIIYQGDIESALQENGINRYMVLNSQLAVIYVPLDFDET
                     ILNNIIQVAWWEESEPMSSLIEITNNVNNGETITTAAETDYIYENPYNDITGRGILLA
                     VIDSGIDYLHPDFINDDGTSKVLYLWDQEANTNPPPEGFIFGSEFTRSELNIAINRND
                     GSLSQDNIGTGTLVSGILVGNGRINSQYRGITTESDLIVVKLKSYTDTYYAGRINYSV
                     SDFLAAITYVTNIARTENKPLIINLTIGVKSSAVATTSILDTFNILSSAGVVVVSGAG
                     NQGNTDIHYSGRFSSVGEVQDVIIQDGDDYALDITLNTNGPDKVGAQIISPSGEVSHD
                     IRYSPDFYIYRGKFNLENTTYAMRFIYPYITSGKENLEIRLRDIKPGVWILRLTSELI
                     INGEYDIYLPNKNLIAPDTRFLEPDSVATITMYAASDDVITVGTFNNKTDSMWIGSSK
                     GPIRGRGIKPDIVASGVDIISTYKNGTYNTGTGTGVSSSIVTGVLALLMEYLEKQDNV
                     PRLSLFTQVLKTYLILGATKLEIYTYPNVSQGYGILNLKNTIQQIANTL"
     gene            complement(5581..8979)
                     /locus_tag="PC471_002994"
     CDS             complement(5581..8979)
                     /locus_tag="PC471_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HDF2790104.1"
                     /translation="MIIINYELIVKYNGDILRLEEELGVSVEILNSSYAIITSSNEED
                     VNILLTYPEIEFIEKPFILQTQDVQSFSSTGITGFKNRTGLTGKGTIIGIIDSGIDYT
                     LPVFRDSDGRSKILYYWDQSIQGNPPEGFREGTLYTNEDINNAIDGSMYIPISTTSLH
                     GTHVAGICATIASDARIIVVRVGNIQTDIFSRSTEFMRAIKFILDRALELRMPVTLNI
                     SYGSNEGSHRGTSLFEQYIDDMCLFWKNNIVVAAGNNADKGGHKRIRLQNNITEEVEF
                     IVGEGERILNINIWPDFVDDFSVHLVNPSNNQTQAISLTSGEIRNTLGETRITGYFYP
                     IAPYSLTRRVTLQLSSNTQITPGLWKIVFEPIDIVTGNVNIYLPTSEGLNRNTRFLIP
                     TQELTVTVPGTASRVITVGSFNSRTDIVSIFSGEGDTQLGVFKPDLLAPGEDIVSFLP
                     GGTSGALTGTSMATPHVTGVCSLFMEWGIVNGNDLFLYSQKLRALLLKGARRLSNQSY
                     PNNSSGFGFLNLSDIDLYTLSNINQDLETEDIGYRSINKSFKDEENSYKFIDGYNMQI
                     HNDLENEIYISKNASRQSGILSGIDIVHTPEFEEELAGLGMSQRFFKISDSLGVLSIN
                     NTDYNSIQRVLQLPSIIRTVSTTKMTLLGEINRGTFGGVVATEEMGVNFFKNNPNINI
                     TGRGTLISIADTGIDYLHPDFIYPDGTSKIVYLWDQTKEGTPPDGFYIGTEYTREDIN
                     RAIAENDPSLSQDEVGQGTMLSGICAGLGNVNSEYAGIAEDSELIIIKLGKIDDFYNS
                     AMLFAASQYAYKKAFELGRPLVINMSLGTSSLAGLTNRSNSEKAFFTRGLCITAGAGN
                     EGNTQTHTSGIIPYVGGSVEVELELNEDEEELSLELWLNRPDKADVIIVSPTGEESKS
                     VGISNYNKVTGLFDLEGTEYSITYIYPTTFSGQQFTNVTLKNAKRGVWKIRLVGVYII
                     TGRYNLYLPNRELLKSGTRFREVDPFYTINYPAIQDDLITIGAYNTINGSLWQSSSRG
                     PTIEDRLKPDIVAPGVNIIAAYPGNTYATITGTAAASAHAAGAAAMYFQYTFVDGRYP
                     NQAYVQKIKTFMQAGARKDSNTVYPNTNSGYGLLDVRGMFDVLR"
     gene            complement(9146..10342)
                     /locus_tag="PC471_002995"
     CDS             complement(9146..10342)
                     /locus_tag="PC471_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HDF2790105.1"
                     /translation="MFIEFFYLLRSRGLNVSLNEWMTLIEALDKGLCYSNFSNFYYLC
                     RMILIKSESDFDKFDAVFLEYFKGIEHQEEIPEEIMNWLKKPDIDLEEFERLEQNPNI
                     NLDKLRAKLEERIKEQDSPHNGGNYWIGTGGTSELGHSGKGQTGIRIGGNSTYGRAVL
                     EVAGERKYRDFRDDEVLNMRQFQVALKRLRQFSTRIDAPKTELDLDKTIEETCNNAGY
                     LKLFFEKPRKNTVKLLLLMDSGGSMRGYSTLCNTLFQSVSKSNHFKDVKIYYFHNCIY
                     DRLFTTPECWLSKSINTEWILKNIDKNYKVIIVGDASMSPSELLHIGGNYRGPYNYTP
                     GIEWLKRFKRKYSKIVWMNPELRDGWDSISYWYQTQRMIQSEFNMFPLTVKGLEKALK
                     NLMVSR"
     gene            complement(10363..11184)
                     /locus_tag="PC471_002996"
     CDS             complement(10363..11184)
                     /locus_tag="PC471_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HDF2790106.1"
                     /translation="MIKKEISNFRGSSDYVVSPELMASVNVAIALEKPLLIKGEPGTG
                     KTMLAQAISNELKKDLVIWNIKSTTKAQEGLYVYDTVQRLYDSQFGGEGVDDISKYIK
                     YGKLGEAFSSNQQVILLIDEIDKADLEFPNDLLWELDKMEFYINETKETVRAKQRPIV
                     IITSNAEKELPDAFLRRCIFHYIEFPDRDMMEEIVKVHFDKVEEHLLEQVMTTFYWIR
                     SLKDIQKKPSTSELIDWIQALTLSGMPIEKIEKEVPFAGILLKNNEDIESMQRHL"
BASE COUNT         3591 a         2064 c         1308 g         4301 t
ORIGIN      
        1 gtagtaaact atctgtaaat cttaattata ataaagtatt aatcaataaa gttactcgac
       61 attatctaat aaattttatc tatcacaatt ttagattgtt acacacaata tattttgata
      121 taattaaatc ataaacgaag tattccaaat atctaatgat agaaaggaat acttattttt
      181 atgctttatt tttaatggag gcttatatga acttagaaaa aataaaaaaa ttttttggat
      241 ttcctctaga caaaaaacac aaagacaatg ttgagaaaga actctgcaat tatctttatc
      301 atacgggtaa aatcatttgc ataataatag taatatctca aataattatg atgatatcaa
      361 tctcagctag agaaggaggc ccttttgtca caccaagacg ccaatggtat ttttatatgt
      421 atgtaacact attttgtgta tcttctgtct ttatgtttgt attttcttac ctttggaata
      481 aaaaaaagga ttgctgtacg ttttatataa atttatggat aatttatgtg acttttttct
      541 gcctatgggg ttgtgtaatt actctacttg accagttagg tggaaatgac ctaagtgtat
      601 ttagttatat tatgttagca gctgcagctt tctcagtatt aaaatttgga gaatcagtaa
      661 tagtttttgg aggatgcttt ttgtttctta atttattctt acctgggatt caaactatac
      721 caggcaatga gtttagtaat attgtcaaca cattttttat aacatccctt gccatttttg
      781 tttctacaat gctatataga aatcgtgtat ttacttacta tgataaactt ttaattagtc
      841 aacaatatga agaaatatct catataaata atttacttaa cgacaaagtt atgacagatg
      901 accttactaa aatgaacaac cgtaggtacc ttgaagatat tctacaaaaa aaattaatca
      961 gccatgcaaa tcaagaacct ataactggaa tgatgataga tatagattat tttaaacaat
     1021 ttaatgataa ttatggacat caaaagggag actattgtct acaaaatatt gccaaagtaa
     1081 ttttagattt tacaagttct gaaaatgaac atgctatacg atatggtggt gaggaatttt
     1141 ttatatgtat ttttaactgt aatgaggcag atgctaaact taaatcagaa tgtttacgaa
     1201 aaattataga gtcctgcaat tttgagcgtg atgatgttcc tttaaaatgt gtaactgtta
     1261 gtattggagt ttatacaaag aaaattggaa agacaataac attaaatgag tttataagta
     1321 attgtgataa ggccctatat actgcaaaag aacaaggacg taattgtgtt gcattttatt
     1381 aaaaggttac atactattac ataaacctat aagtattata tcgcaaaata aaagtaatca
     1441 aattatatat ttatagtaca ataaaaataa cttgcatatc tctaaaatag ataaatatgc
     1501 aagtcatttt agtgtttata cttaaagtta tataagtttt attatatata attattaaaa
     1561 ttcagcattt ttaggagttc ttgggaaagg tataacgtct cttatgttag acatacctgt
     1621 catatacata attattcttt caaatccaag accaaatcca gagtgagtag cagtaccaaa
     1681 ctttctaagt tctaaatacc accaataatc ttcttctctt aatcccattt cattaattct
     1741 atctaacaat acatcttctc tttcttctct ttgagaacct cctacgattt ctccaactcc
     1801 aggaactagt aaatccattg ctctaacagt tttaccatct tcattcattc tcatatagaa
     1861 agcttttata tcttttggat aatctgtaac aaacactgga caattatata tttgctccgt
     1921 tatatatctt tcatgctctg tttgaaggtc acatccccac tcaacaggat actcaaattc
     1981 atgcccagat tcaattaata attccactgc tttagtatac gttattctaa taaactcaga
     2041 gtttactatc ttatttaatc tttctaacaa tcctttatct ataaaactat taaaaaattc
     2101 catttcttca ggagcatttt ctaatacata gcttataaca tattttatca tatcttctgc
     2161 aagttccatg ttatcttcaa ggtctgcaaa aactatttct ggctctatca tccaaaattc
     2221 tgaagcatgt cttcctgtat aagagttttc agctctaaat gtaggtccaa aagtataaac
     2281 gtttctaaaa gaaagtgcca ttatctcggc atttaattga ccactaactg ttaaattagc
     2341 ctcttttcca aagaaatctt gagtatagtc tattttgcca tcttcagttt ttggaatatc
     2401 atttaaatcc atagttgtta ttctaaacat ctctcctgca ccttcacagt cactacctgt
     2461 tatgattgga gaatgtgcat atacaaagtt tctttcttgg aagaatttgt gtattgcata
     2521 ggcagctatt gatcttactc taaatacagc tgaaaaagta ttacttcttg gtcttaaatg
     2581 tgctatcgtt cttaaatatt caagcgtatg tctcttcttt tgtagtggat acgaactatc
     2641 tgattcccct tctacaacta tgctttttgc atgtatttcg actggttgtt tagccccttc
     2701 tgtagatact aactctccct ctacaagtat agatgagctt attgctaatt ttcctacctc
     2761 tttaaaattt tctactttct catcaaatac tatctgcata tttttaaaga agcttccatc
     2821 atttagctct ataaatccaa aatttttaga tactctagat gttcttatcc atccagctac
     2881 ttttacagtc ttccctatgt actgttcttt ttctctgtac aattctttta ctgttataaa
     2941 ttccttttgc attttaaatc ctcctagtat aattgttatt aatttctttt aaatataatt
     3001 tcaataaaaa aagcccttca tccctatata aagggacgaa agactattac tctcgcggta
     3061 ccaccctagt tgtcataatg acctcttagt taagcaatgc ttaaagccta taacggtgcc
     3121 ttccgtctaa gtctacttac taaataagct ttcgattaga aactccaaga tgttcttcaa
     3181 tataaacctc gtattgagct ttcaccatcc tcaactcgct agaactacat tttatatcta
     3241 ctcttctctt cacagtattt atcttttatt atttctgcta attaacaaaa ttataccaat
     3301 ttataaattt ttagtcaata aatttatata attgatactg attgttattt aaaatataat
     3361 aattctacct tacataatga taataaatac ttatcttcat tacttcttta ttacttcaat
     3421 ttccaaaatt ttataattta cttccattgt caacccagtc ttttgcttct tcgacaccct
     3481 taattgaagg tattgaaact ttactatgag gttttaattt ttcaacatca gcccatgctg
     3541 ctgtcgactc atttccaact aaaagtgaac ttttaagtct tttattatct ttacctttta
     3601 atgctcttgc ctttttactt tccaaaacta tcacctctat tatttatttt gtaatagttt
     3661 tagcaatttt acaaataaaa ttcataagtc taaattataa ttatttttaa attaacataa
     3721 tatcttttca atacttaatt tatacctatt tatatacaaa atatttactt tgtgtattca
     3781 aaatataaaa ctgtttttta attttcatat ttatttttta tgctattttt ctactctatc
     3841 taaagtattt gatacaatta aactaataat caattataat tttacatagg ttcttatcta
     3901 tagagtattt gctatctgtt gaatcgtatt ttttaaattt aaaatcccat atccctgcga
     3961 tacatttgga tatgtatata tctcaagttt tgtagcaccc aatattaaat atgtttttaa
     4021 aacttgtgta aacaatgata atctcggtac attatcctgt ttttctaagt attccatcaa
     4081 taaggctaaa acacctgtga ctatagaact gctcactcct gttcctgttc ctgtattata
     4141 tgttccattt ttatatgtgg atattatatc tacacctgaa gctactatat ctggcttaat
     4201 tcctcttcct ctaattggtc ctttcgaaga accaatccac atactatcag tcttattgtt
     4261 aaatgtacca actgtaatta catcatcact agcagcatac atagttatag tagctactga
     4321 atcaggctct aaaaaccttg tgtctggagc tatcaaattt ttatttggca agtatatgtc
     4381 atattctcca tttattataa gttctgaagt caatcttaaa atccatactc caggttttat
     4441 atctcttaat cttatctcca aattttcttt tccagaggta atatatggat aaataaatct
     4501 cattgcataa gtagtatttt ctaagttaaa ttttcctcta tatatataaa agtctggaga
     4561 ataccttata tcatgactaa cttcacctga aggagatatt atctgtgctc ccactttatc
     4621 tggaccattc gtatttagtg ttatatcaag agcatagtca tctccatctt gtattattac
     4681 atcttgaact tctcccacac tgctaaatct gccagaataa tgaatatctg tattcccttg
     4741 atttcctgca ccactcacta ctaccacacc agcactactc aatatattaa aagtatctag
     4801 tattgaagta gttgctacag cacttgactt aactccaata gtcaaattta ttatcagtgg
     4861 tttattttct gttcttgcta tgttagtaac atacgttata gctgctaaaa aatctgaaac
     4921 actgtaattt atccttccag cataataagt atctgtatat gattttaatt taactactat
     4981 taagtctgat tctgtagtaa tacctctgta ctgagaattt atccttccat ttccaactaa
     5041 tataccagaa actaaagttc cagtacctat attatcttga ctcaaactcc catcatttct
     5101 atttatggct atatttaact cacttcttgt aaattcactt ccaaaaataa aaccttctgg
     5161 aggtggattt gtatttgctt cttggtccca taaatataaa actttactag ttccatcatc
     5221 atttataaaa tcaggatgca aataatctat cccagaatct ataactgcta ataaaattcc
     5281 tctccctgtt atatcattat atggattttc ataaatataa tcagtttcag ctgctgttgt
     5341 aatggtttct ccattattta cattattagt tatttcaatt agactgctca ttggttcaga
     5401 ttcttcccac caagcaactt gtattatatt atttaaaata gtttcatcaa aatctaaagg
     5461 tacatatatt actgcaagct gactatttaa aaccatatac ctatttattc cattttcttg
     5521 tagggcagat tctatatcac cttgataaat tatacaataa gatttttcca taaatccctc
     5581 ctatcttaaa acatcaaaca ttcctctaac atccaagagc ccatatccag aatttgtatt
     5641 tggatatact gtgtttgaat cttttctagc accagcttgc ataaaagttt ttattttttg
     5701 cacatatgct tggtttggat atctaccgtc aacaaaagta tattgaaaat acattgcagc
     5761 agcaccagct gcatgtgcac ttgctgctgc tgtaccagtt atagttgcat acgtgttccc
     5821 aggataagca gctattatat tgacacctgg tgctactata tctggtttta acctgtcttc
     5881 aatagttggc cctcttgatg aactttgcca taaactacca ttaatggtat tgtaggctcc
     5941 tatggttatt aaatcatctt gaatcgcagg ataatttata gtgtaaaaag ggtctacctc
     6001 tctaaatcta gttccacttt ttaaaagttc tctatttgga aggtataagt tatatcttcc
     6061 tgttataata taaactccta ctaatcgaat cttccataca cctctttttg cattctttaa
     6121 agtaacatta gtaaattgct gtccagaaaa agttgttggg tatatatatg ttatagaata
     6181 ttcagtcccc tctaaatcaa ataaacctgt tactttatta taatttgaaa ttccaacact
     6241 tttactttct tcaccagtgg gagatactat tattacatca gctttatctg gtctatttaa
     6301 ccaaagttct aatgataatt cttcctcgtc ttcatttaac tctaattcaa cttcaacaga
     6361 acctccaaca tatggtatta ttccagatgt atgtgtctgt gtattgccct catttcctgc
     6421 tcctgccgtt atacataacc ctctagtaaa gaacgctttt tcactattac tcctattggt
     6481 taaacctgct aggctactgg tacctaaaga catatttata actaatggtc ttccaagttc
     6541 aaaagctttt ttatatgcat actgagatgc agcaaacaac attgcactat tataaaaatc
     6601 atctatcttg ccgagtttta ttattattag ctcagaatct tctgctatac ctgcatattc
     6661 actatttaca tttccaagcc ctgcacatat cccactcaac atagttcctt gaccaacttc
     6721 atcctgagat agactagggt cattttctgc gatagctcga tttatatctt ctcgtgtata
     6781 ttctgtccca atataaaaac catcaggagg agttccttct tttgtttggt cccataaata
     6841 tactattttt gaagtgccat ctggataaat aaaatctggg tgtagatagt ctattcctgt
     6901 atctgcaatt gatattagag tccctcttcc tgttatatta atattaggat tatttttaaa
     6961 gaaatttacg cccatttctt ctgttgcaac tacacctcca aaagtacctc gattaatttc
     7021 tccaagtaat gtcatctttg tagtcgatac agttcttatg attgaaggaa gttgcaaaac
     7081 tcgttgtatg ctattatagt ctgtattatt tatagatagt acacctaatg aatcacttat
     7141 cttaaaaaac ctttgactca tacctaaccc agctagttct tcttcaaatt caggagtatg
     7201 gactatatct attcctgata gtattcctga ttgtctactt gcatttttac ttatataaat
     7261 ttcattctct aagtcattat gtatttgcat attataacca tctataaact tataactatt
     7321 ttcctcatct ttaaatgact tattaatact tctatatcca atatcctctg tttctaaatc
     7381 ttgatttata ttagatagag tgtacaaatc tatatctgat aaatttaaaa aaccaaatcc
     7441 tgatgaatta tttggataag attgattact taatctcctt gctcccttta gaagtaatgc
     7501 ccttaatttc tgtgaataca aaaataaatc atttccattt acaattcccc attccataaa
     7561 caatgaacaa acccctgtaa catgaggtgt agccatgcta gttccagtta atgctccact
     7621 tgtacctcct ggtaaaaaag atactatatc ttcacctgga gctaataaat caggtttaaa
     7681 aacacctaat tgtgtatctc cttcaccaga aaatatagat actatatctg ttcttgaatt
     7741 aaaactacct actgttataa ctctacttgc tgttccagga actgttacag taagctcttg
     7801 agtaggaatc agaaatctag tatttctatt tagaccttca gaagttggca aatatatatt
     7861 tacattacca gttactatat ctattggttc aaatacaatt ttccataacc caggagttat
     7921 ctgtgtattt gaacttaatt gtagagtaac tcttcttgtg agagaatacg gtgctatagg
     7981 gtaaaaataa ccagttattc tagtttcacc caaagtattc cttatctcac ctgaagttag
     8041 agagattgct tgtgtttgat tatttgatgg gtttaccaaa tgtacactaa aatcatcaac
     8101 aaaatctggc catatattta tgtttaatat acgctctcct tccccaacta taaactcaac
     8161 ttcttctgta atattatttt gaagcctaat ccttttatga cctcctttat ctgcattatt
     8221 tccagctgct acaactatat tgtttttcca aaataaacac atatcatcta tatattgttc
     8281 aaataaagat gttcctctat gtgaaccttc attacttcca taacttatat ttaatgtaac
     8341 aggcattctt aattctaatg ccctatctaa aataaattta attgccctca taaactctgt
     8401 acttctcgaa aaaatatcgg tctgtatatt tccaactcta accactatta ttctggcatc
     8461 acttgcaata gttgcacaaa tacctgcaac atgtgttcca tgtaagctag ttgttgaaat
     8521 aggtatatac atacttccat ctattgcatt atttatatct tcattagtat ataatgttcc
     8581 ttctctaaaa ccttctggtg gatttccttg aatagattgg tcccaataat ataatatctt
     8641 agaccttcca tcactatctc taaacacagg tagagtataa tctattcccg aatcaattat
     8701 accaattata gttccttttc cagttaaacc agttctattt ttaaatcctg ttattcctgt
     8761 acttgaaaaa ctttgcacat cctgagtctg taatataaaa ggcttttcta taaattcaat
     8821 ttctggataa gttaaaagta tatttacatc ttcttcatta cttgatgtta ttattgcata
     8881 tgatgaatta agtatctcta ctgatacacc taattcttct tctaacctca atatatctcc
     8941 attgtatttt acaattaatt cataatttat tataatcacc tcgacttcta aaattattat
     9001 taatgtatat gttttgtgtg agtctaaaat gtattagact atataatttt ttaagtttca
     9061 gttatacaat ttataataga tatattaaaa aaataaataa tgatggtaca attttgaata
     9121 aatcatacca tcatttatat ttactttatc ttgaaaccat taaatttttc aatgctttct
     9181 caagaccttt tacagtcaat ggaaacatat taaattcact ttgtatcatt ctttgagttt
     9241 gataccaata actaattgaa tcccatccat ctctaagttc tggattcatc catacaattt
     9301 tactgtactt tctcttaaac cttttaagcc actctatacc aggtgtataa ttatatggac
     9361 cacgatagtt accaccaata tgaagcaact cagaaggcga catagaagca tctccaacaa
     9421 ttataacttt atagttttta tctatatttt ttaatatcca ttcagtatta atagatttac
     9481 ttaaccaaca ttcaggtgtt gtaaataatc tatcataaat acagttatgg aaataatata
     9541 tctttacatc tttaaaatga tttgatttac ttacagactg aaataatgta ttacataaag
     9601 tgctataccc tctcattgaa ccacctgaat ccataagcaa taaaagcttc actgtatttt
     9661 ttcttggctt ctcaaaaaat aactttaaat atcctgcatt attacaagtc tcttcaatag
     9721 tcttatccaa atcaagctct gtctttggag catctattct agtagaaaat tgacgaagtc
     9781 tctttagtgc aacttgaaat tgacgcatat ttaaaacttc atcatctcta aaatctctgt
     9841 attttctttc tcctgcaact tcaagaacag ctctaccata agtagaattt ccacctatac
     9901 gtataccagt ttgccctttt cctgaatgtc ctaactctga agtgcctcct gtgcctatcc
     9961 aataattacc accattatga ggagaatctt gttcttttat tcgttcctcc aatttagctc
    10021 ttaatttatc aagatttata tttggatttt gttctaatct ttcaaactct tctaaatcta
    10081 tatctggttt tttaagccaa ttcataatct cttctggaat ttcttcttgg tgttctatac
    10141 ctttaaaata ctccaaaaac actgcatcaa acttatcaaa atcactttca ctttttatta
    10201 aaatcatcct gcaaagatag taaaaatttg aaaagtttga atagcacagt cctttatcca
    10261 atgcttcaat taaagtcatc cattcattta aagatacatt taatcctctt gacctcaata
    10321 aataaaaaaa ttcaataaac ataattatcc agctcctatt aactataagt gtctttgcat
    10381 agattcaata tcctcattat tcttaagtag tattcctgca aaaggaactt ctttttctat
    10441 tttttcaata ggcattccac tcaaagttaa tgcttgtatc cagtctatta actctgatgt
    10501 actaggcttt ttttgtatgt cctttaaact tctaatccaa taaaaagtag tcattacttg
    10561 ttccaatagg tgttcttcta ctttatcaaa atgtacttta actatttctt ccatcatgtc
    10621 tctatcagga aattctatat aatgaaatat gcatcttctt aaaaaagcat ctggaagttc
    10681 tttttccgca tttgaagtta taataactat tggtctttgt ttagctctta cagtttcttt
    10741 tgtctcattt atgtaaaact ccattttatc caattcccaa agcaagtcat ttggaaattc
    10801 taaatccgcc ttatctattt catctattaa aagaattact tgttgatttg agctaaaggc
    10861 ctctcctagt ttaccatatt ttatgtactt gctaatatca tctacacctt ctcctccaaa
    10921 ttggctatca taaagtcttt gaacagtatc atatacataa agcccttctt gtgcttttgt
    10981 agttgatttt atattccaaa ttactaaatc tttttttaat tcatttgaaa ttgcttgtgc
    11041 tagcatagtt ttacctgttc caggttcgcc cttaatcaaa agtggctttt caagagcaat
    11101 agcaacatta actgatgcca tcaattctgg tgatactaca taatcactac ttcctctaaa
    11161 gttagatatc tcttttttta tcataaatcc tactcctttt aaacagtatt ttctaaatgt
    11221 atatagactt tattactatt tttgatataa aaataataat acaa
//
LOCUS       DAMJYR010000118        11196 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.122, whole genome shotgun sequence.
ACCESSION   DAMJYR010000118 DAMJYR010000000
VERSION     DAMJYR010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11196)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11196)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11196
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.122"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            <1..1100
                     /locus_tag="PC471_002997"
     CDS             <1..1100
                     /locus_tag="PC471_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HDF2790107.1"
                     /translation="IKQSTVLSIVSTLLVYLIEQLVYGFTAPLNYLIFTSDINTDIYK
                     FDINMGLSSLITIIIAGFIYWYSSKKFNIKVMNFDKYIAILMIPLLLIILFMQSFEYS
                     SNINIDTSLGIVKLLLNTSITEEIQAFLFSIIGTICVFFSLFTFKKLIQALEDDKERA
                     IMNQQIHAQKNYIEEAKSRLSQTISFRHDFNNHLAIVNGLLKKDQILKAQDYLNKLEK
                     VTSDLSFSCNTQNIVIDALFNNKLSIAKQLGIEVDCEVIIPQSTNMNDFDLCIVFANA
                     IDNAIKACRVVDKQNRYLKLSTKLEGGFFMIEIKNSYNPINNYTSGSGIGLLNIKEIA
                     KKYQGAISIDKTSDYFQINVLLVISLHCASI"
     gene            1139..1624
                     /locus_tag="PC471_002998"
     CDS             1139..1624
                     /locus_tag="PC471_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2790108.1"
                     /translation="MYLKRESLIYRNEEVTKMKVGNINGQAPITNGLDSCQSEVQGLE
                     QKKKALQDEIEKVRSDKSIDGETAKLKIEALKKEMEEIENKIQELKGNKGKKTNKEEN
                     NVSEVNNNLLERKKEPSNNENVKQLYDVFERSNVDEQWDNTYRVLQDDKNLKVKFNRP
                     I"
     gene            complement(2279..2503)
                     /locus_tag="PC471_002999"
     CDS             complement(2279..2503)
                     /locus_tag="PC471_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HDF2790109.1"
                     /translation="MTVYNLKLGQKGIIDNIAGNEKLMKRLLALGLIDGTEVEVKKIA
                     PLNDPIVIRFRGFDLAIRKSDAKNINLKNN"
     gene            complement(2600..3853)
                     /locus_tag="PC471_003000"
     CDS             complement(2600..3853)
                     /locus_tag="PC471_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:glutamate symporter"
                     /protein_id="HDF2790110.1"
                     /translation="MITLSFDIVQTLTLSIFFFLIGNLLKNKVNFLNNFCIPAPVIGG
                     LLFCFLNLFFKYFNIADISISGNLMPNFITFFFTTIGLEISINLIKKGGSVLFRYWIL
                     CGVLAFCQNILAITISKIIKLEPLLGLMCGNVSMEGGHGYSAAFGLTIENLGIEGAVG
                     VGLSAATIGLIMGGILGCPVAKFLINKYKLKPSSNIDLSIPRYNRNLKRFGSKFFRNK
                     NRIKNNNFSETITPSVFLEQVLLIFICINTGEIISRCFYITFNILLPSVVTCMFSAVI
                     FRNLNDKINILELNFKLIDFLKELSLGIFLTLSLMNIDLFELSTLLPPILLIVTFQVI
                     FIILFSIFICFRVLGKDFDSAIIISGLIGHGIGATPNALANMSSLTQKYGDSPKAFLV
                     VPLVSGFLLDAISIPCILFFINILT"
     gene            4113..5102
                     /locus_tag="PC471_003001"
     CDS             4113..5102
                     /locus_tag="PC471_003001"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HDF2790111.1"
                     /translation="MFENLPKIDLHCHLDGSVRVETMLDIAIKEKIDLPSNNMDEIKK
                     LAKVSFNCTSLDEYLEKFDLPLKVMQSKENLKRITFELLEDASRENVKYIEIRFAPLL
                     HTQKGMSVKNIIEGIIEGIREAESIYDIKGNLILGCMRTMTSKEALLVIEEGKSFVNK
                     GVVAVDLCGPEKEGFCKEYKDVFKLAREYGYKVTIHAGEAASGENVLDAINILKADRI
                     GHGVKIKDHKKAYNLVKDKKILLELCPTSNVQTKTVDSYEAHPFYTFYKDNLHVSINT
                     DNRTVSDINLSSELNVIFDTFKLGLEDYKIIYRNAVEASFADKETKEYLNSLI"
     gene            5174..5914
                     /locus_tag="PC471_003002"
     CDS             5174..5914
                     /locus_tag="PC471_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EcsC family protein"
                     /protein_id="HDF2790112.1"
                     /translation="MKDYEDVVYIKLVKKELEEWKKNILKKPSIFDKASKGIQNKFNG
                     VLPDKYHEIITSSIKVMTKTVLFGYKYATKPSLRNMSLEEREKLVLEKAKTYKTTAMI
                     EGAGTGAGGILLGLSDFPLLLGIKVKFLYDVASIYGYDVKDYRERIYILNILQLAFSS
                     QQHINKTFKLMNNWDEYVNSLPEDINDFDWRTFQQEYRDYLDLAKFLQLMPGIGAFVG
                     FYVNGKLIDKLCETAVSAYRLRIRELKL"
     gene            6114..7265
                     /locus_tag="PC471_003003"
     CDS             6114..7265
                     /locus_tag="PC471_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase"
                     /protein_id="HDF2790113.1"
                     /translation="MTNLPKQFDSFNEARQKGFINAKELKESGKKMVGVFCTFTPVEI
                     PMAAGATVVGVCGVSEEPIPDAERVLPRNLCPLIKSSYGHAITDTCPYFYFSDLLIGE
                     TTCDGKKKMYEELAKVKPTYVMHLPNTSKGEFAYKLWKDEMIRLKEEVEKSLGVTITE
                     EDIRTAIKDKNEERELLKEFYALGKLQPSALTGLELHNVLYQAGFKFDRAELKHSLRK
                     VIDDMKERYEKGECPVQKDKPRILITGSPIGGISEKIVKTLEDAGASVVAYELCGAIR
                     SNDLLVDEEIEDVYDALTQKYINIGCSCMMNNDNRIELLDRIIDEYNVDAVIDVVLQA
                     CHTFNIESYRIREFVTKEKNKPFMSLETDYSKSDTEQLRTRFEAFVEML"
     gene            7290..8045
                     /locus_tag="PC471_003004"
     CDS             7290..8045
                     /locus_tag="PC471_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyglutaryl-CoA dehydratase"
                     /protein_id="HDF2790114.1"
                     /translation="MFSIGVDSGSVATKGVLFDGEKIIKKIIIPTGWSPKSTSKQVYE
                     LLSSEIDKKDIKKVVGTGYGRGVMDFADKKVTEITCHTRGIYFLNKNIRTILDVGGQD
                     SKVINLDRDGNVFNFIMNDKCAAGTGRFLEITSNLLGSDIESIDTLAKGYEPVNISSM
                     CTVFAESEIVSLLAQNISTGEVAAGILKSIANKSTSMLARGEVIDEVAFTGGLAKSKE
                     LVKMIEEILGKKIFIAEDTQIIGALGAAVIGFR"
     gene            8237..10624
                     /locus_tag="PC471_003005"
     CDS             8237..10624
                     /locus_tag="PC471_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding cysteine protease Cwp13"
                     /protein_id="HDF2790115.1"
                     /translation="MKKFTSKKVTRFVALFLISILVMSTIPVSADNSNTLNQIKKAEY
                     SETYKQYLNDAKNGRTEKYNGIIPNPYQLEGTQIQSKGRTAPTSYDPRKLGLMTSIKN
                     QEDLGICWDFAAMATLESFLKLNNYGDYDLSEEHLRWWASDGEYGWSVNDMNGALNYE
                     AMGYLTSWSGPKLEKDIPYNGRVSKAQGAKKPTNMNTAPTVFNVTDAVCVSNDINSTK
                     NAILQYGAVTSGYYEDIKYQSDDQNSYYCPTKSFNTNHAISIVGWDDNYSKDNFNANI
                     RPSKNGAWLIKNSWGDYNSEGGYFWISYEDKTLMSDIDNFSIKGGKKPNDDEKMYQHD
                     YASIVPLISKKITAANVFDFNRGDETLKSVMFLTESIGAKYEVYYAPVVNGIPQENNM
                     KKLKEGTAQYSGYITVPIDSFDIPEGKGAIVVSIEGKNGESTIGSESNVPGYDTFKAK
                     ANLGESYIIDNTGKFFDINRDSNFYPCNFTIKAVTEKSSGESIPNESLIGSDRYETAI
                     KVSQNGFNSSENVVLVNGSSIVDALAATPFTSAINSPILLTQKEALNSKTKAEIQRLG
                     AKKVYLIGGENSISKEIEQQLKSLNISIERISGSDRYKTSLLLAQKLNGIKNVSQIAV
                     VNGVKGLADAISVGAAAAENNIPIILANEKSELQGADEFLNSLNIEKSYIIGGTASLS
                     NNLESKLKNPTRLSGSSRDETNSKIIDNFYKKDTLKNAFVVKNGIKNQNDLIDGLAVG
                     PLGAKTGSPVILVGDKLADSQKEVLKNKTLEKVTQVGGGANKNAFKELVKLKSAK"
     gene            complement(10855..11088)
                     /locus_tag="PC471_003006"
     CDS             complement(10855..11088)
                     /locus_tag="PC471_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HDF2790116.1"
                     /translation="MATTTKNRFYDYVQENLNKYVEVHSYFSSCPLYGEIIEADSLSI
                     TLCSRYIDESNPTLNETYTLYLPLSSIISIRTL"
BASE COUNT         4493 a         1242 c         1824 g         3637 t
ORIGIN      
        1 taataaaaca atctactgtt ttatctatag tatcaacttt gttagtatat ttaattgaac
       61 aattagtata tggatttaca gctccattaa attacttgat atttactagt gatataaata
      121 cagatattta taaatttgat ataaatatgg gactatcttc tttaattact attataatag
      181 ctggatttat atattggtat agttctaaaa agtttaatat taaggtcatg aactttgata
      241 aatatatagc aattttgatg attccattgt tgcttataat attatttatg caatcatttg
      301 aatatagttc aaatataaat atagatacaa gtcttgggat tgtaaagctg ctattaaata
      361 cttctataac tgaagaaatt caagcttttc ttttttctat aattggaact atatgtgtat
      421 ttttttctct atttaccttt aagaaactaa ttcaagctct cgaagatgac aaggagaggg
      481 caattatgaa tcaacagatt catgctcaaa aaaactatat tgaagaagcg aaatcacgat
      541 tatcacaaac tatatccttt agacatgatt ttaataatca tttagctatt gtcaatggat
      601 tgttgaaaaa agaccaaatt ttaaaagcac aagattattt aaataagctt gaaaaagtaa
      661 caagtgacct atcattttct tgtaatacac agaatattgt tattgatgct cttttcaaca
      721 ataaattaag tatagcaaaa caattaggca tagaggtaga ttgtgaagta attatacccc
      781 aaagcactaa catgaacgat tttgacctat gtatagtgtt tgctaatgct attgacaatg
      841 caattaaagc ttgtagagtt gtagataaac aaaataggta tttaaagctt tccactaagt
      901 tagagggtgg attttttatg atagaaataa aaaatagcta taatccaata aataattata
      961 caagtggctc aggtataggt cttttaaata taaaagaaat tgctaaaaaa tatcaagggg
     1021 ctataagtat agataagaca tcagactatt ttcaaataaa tgtattgcta gttatttcat
     1081 tacattgcgc tagtatttga gaacaatttc attgaaaata gtatttgtaa gctatattat
     1141 gtatttgaaa agagaaagtt tgatatatag aaatgaggag gttactaaaa tgaaagttgg
     1201 aaatataaat ggtcaagcac caattacaaa tggtttagat agttgtcaaa gtgaggtaca
     1261 aggcttagaa caaaagaaaa aagcattaca ggatgaaata gagaaagtaa gaagtgataa
     1321 aagtattgat ggtgaaacgg ccaaattaaa aatagaagct ttaaaaaaag aaatggaaga
     1381 gattgaaaat aaaattcaag agttaaaagg aaataaagga aaaaaaacaa ataaagaaga
     1441 aaataacgtt tcagaagtaa ataataatct attagagaga aaaaaagaac cttctaataa
     1501 tgaaaatgta aaacaattat atgatgtttt tgaaagaagc aatgtagacg aacaatggga
     1561 taatacttat cgtgttctcc aagatgataa aaacttaaaa gtaaaattta atagacctat
     1621 atagtttatt tataaaaata gttaatattt taattatcac cactttttag aagaatacac
     1681 actttatttt aaagacccac atatttattt acagttagca catttatatg tggttttata
     1741 actattattt acaagtctat attgataact tttacatagt atatgatgtt taaatactta
     1801 ttcaaattta tctatacata aacaaaaaaa gagattttaa aatctctttt tagattgttg
     1861 acttatggct ttttgattta ataaaatcaa gaagccgttt gaattttata tgttggctga
     1921 tacccttctg atattgaaac accatttttg tttccaaaat cgtttaaaat taagaactta
     1981 ttatgaatga ctatttcatt atgaacaata agcaaataac atgaatcttc aatcaatgtt
     2041 ttaacagtag cattaaatgc ttgcttatat agttctatcg tttgtttgaa ctgcccatta
     2101 aagtaaatat gagtcaataa cataactata tctacacaaa ttaatataaa ttttttatac
     2161 tgtacattat ctaactgttg attatctgta ttacaacatg attgaattta aaaagccatc
     2221 aacacataga atgttgatgg cctgaaaata gattttaaaa tctctttttt attatatatt
     2281 aattattttt taaatttata ttttttgcat cagattttct aatagctaag tcaaaccctc
     2341 taaatctaat cactataggg tcattaagag gagctatctt tttcacttca acttcagtgc
     2401 catctattag tcctaaggct agaagtctct tcataagttt ttcatttcca gctatattgt
     2461 ctataattcc cttttgtcca agttttaagt tgtaaacagt cattattaac acctcctaat
     2521 gaaaatcatt atcaaacttt atatttataa tatataacaa aaagagcaaa ttgtcaatat
     2581 ataacaaaaa ataaacatac taagttagta tgtttataaa aaatagtata catgggatac
     2641 taatagcatc tagcaaaaaa ccacttacta atggtacaac taaaaaggct tttggagaat
     2701 caccatattt ttgtgtcaaa gaactcatat ttgccagagc gttcggagta gctcctattc
     2761 catgtccaat caaaccactt attatgatgg cagaatcaaa atcttttcca agtactctaa
     2821 agcaaataaa tattgaaaat aggattataa agattacttg aaaagttact attaagagaa
     2881 taggaggtaa taatgtagat aattcaaata aatctatatt cataagtgat aaagttaaaa
     2941 aaattcccaa tgaaagttct tttaaaaaat ctataagttt aaaatttaat tctagtatat
     3001 ttatcttatc atttaaattt ctaaaaataa cagccgaaaa catacaagtt acaacagagg
     3061 gtaatagtat attaaacgtt atatagaagc atctacttat aatttctcct gtatttatac
     3121 aaataaatat gagaagaacc tgttccaaaa atacagatgg tgttattgtt tcggagaagt
     3181 tattattttt tatcctattc ttatttctaa aaaatttact tccaaatctc tttagatttc
     3241 tattatatct aggaatcgac aagtctatat ttgatgatgg ttttaattta tatttattta
     3301 ttagaaattt agctacagga catcccaata taccacccat aataagtcct attgttgcag
     3361 cagataatcc aaccccaact gctccttcta taccaagatt ttctattgta agtccaaatg
     3421 cagcagaata accatgacca ccttccatag atacatttcc acacataagc cctaaaagag
     3481 gttcaagttt tataatcttt gatattgtaa tagctaatat attttgacaa aatgccaata
     3541 ctccacacaa aatccaatat ctaaataata cactaccacc tttctttata agatttatgc
     3601 ttatttcaag tcctatagtt gtaaaaaaga aggttatgaa atttggcatt aaatttccac
     3661 ttatagatat atctgctata ttaaaatatt taaaaaataa atttagaaaa caaaataata
     3721 gacctcctat tactggtgca ggtatacaga aattatttaa aaagttaact ttattcttca
     3781 ataaattacc aattaaaaag aagaatatag atagtgtcaa ggtttgcact atatcaaaac
     3841 ttaaggtaat caaaataagt cctcctttgt aattcaaagt tttaattata taacttaaat
     3901 tcttatgtat tgaaatttta ttatcaaatg tatattaata aatgtatatt aaaaagaatc
     3961 agtagatata attagatagt cataaattaa attaaaatat tttaatgaga tatgactttt
     4021 ttagaattaa aaaccgtagt atattgatat aatagaaata agacttaaaa atctgttgat
     4081 agtattattt ataagtacag taggaggata aaatgtttga aaatttacct aagatagatt
     4141 tacattgcca cttagatgga agtgttagag tagaaaccat gcttgatata gctattaaag
     4201 agaaaataga tttaccttca aataatatgg atgaaataaa aaaattagca aaggtttctt
     4261 ttaattgtac ttcactagat gaatatttag aaaaatttga tttaccctta aaagtaatgc
     4321 aatctaagga aaatttaaaa agaattactt ttgaactttt ggaagatgca agtagagaaa
     4381 atgtaaaata tattgaaatt agatttgcac cattacttca cacccaaaaa ggtatgagtg
     4441 ttaaaaatat aattgagggt ataattgaag gaattagaga agctgaaagt atttatgata
     4501 taaaaggcaa tttaatatta ggttgtatga gaacaatgac ctcaaaagag gcattattag
     4561 taattgaaga agggaaatca tttgtaaata aaggtgttgt agcagtagac ttgtgtggtc
     4621 cagagaaaga gggattttgt aaggaatata aagatgtttt taaattagca agagaatatg
     4681 gatataaagt tacaatacat gcaggagaag ctgcaagtgg agaaaatgta ttggatgcta
     4741 taaatatttt aaaggcggat aggattggtc atggggtaaa aattaaagac cataaaaagg
     4801 cgtataattt agttaaagat aaaaaaatat tgcttgaatt atgtccaact agtaatgtac
     4861 aaactaagac agtagattct tatgaagcac atccatttta tactttttat aaagataatt
     4921 tacatgtaag tataaatact gataatagaa ctgtatcaga tataaattta agttcagaac
     4981 taaatgttat atttgatact tttaaactag gattagaaga ctataaaata atttatagaa
     5041 atgcagtaga agctagtttt gcagataaag aaactaaaga gtatttgaat agtttaattt
     5101 aatagataaa aatatttaaa ctttaaatta agctattaaa ttgataaaaa tacattgtat
     5161 taggagaaaa aatgtgaaag attatgaaga tgttgtttat attaaactag tcaaaaaaga
     5221 acttgaagaa tggaagaaaa atatattaaa gaaaccttct atatttgaca aagcatcaaa
     5281 gggtatacag aataaattta atggagtatt gccagataaa tatcatgaaa taattacttc
     5341 ttctataaaa gtaatgacaa aaaccgtact gtttggatat aaatatgcta caaagccatc
     5401 acttagaaat atgtcattag aagaaagaga aaaattagtt ttagaaaaag ctaaaactta
     5461 caagactaca gcaatgattg aaggggcagg aacaggggca ggaggaatcc tcttaggttt
     5521 atctgatttt cctttgcttt taggtattaa agtgaaattt ttatatgatg tagcttcaat
     5581 atatggatat gatgttaaag attatagaga aaggatttat atattaaata tattacagtt
     5641 ggctttttct agtcaacagc atataaacaa aacttttaaa ttgatgaata actgggatga
     5701 atatgtaaat agtctaccag aagacatcaa tgattttgat tggagaacat ttcaacaaga
     5761 gtatagggat tatttagatt tagctaagtt tttacaacta atgcctggaa taggagcatt
     5821 tgtaggattt tatgtgaatg gaaaattaat tgataaatta tgtgaaactg ctgtgtctgc
     5881 ttatagattg aggattcgag agcttaagtt ataagagaaa ttataacata aaaaattagt
     5941 tatacaatat atctatatgt atctaatttt gcattgataa aaacaattat ggataaaagg
     6001 taaagcatga tagaatctaa tgtagaggtt tgtattttaa tggaaaaatt tagtttttat
     6061 tgcttaagat acaaattgaa ttacgaagag aagtaattgg gaggaatatt ttcatgacaa
     6121 atttgccaaa acaatttgat agcttcaatg aagctagaca aaaaggattt ataaatgcaa
     6181 aggaacttaa ggaaagtggg aaaaagatgg ttggagtttt ctgtacattt actccagttg
     6241 aaattccaat ggcagctgga gcaactgtag taggtgtatg cggcgttagt gaagagccaa
     6301 tacctgatgc agaaagggtg cttccaagaa atctttgtcc tttaattaaa tcaagctatg
     6361 gacatgcaat aacagatacc tgtccttatt tctatttttc tgatttgttg ataggagaaa
     6421 ccacttgtga tggtaagaaa aaaatgtatg aagaacttgc aaaagttaag ccaacatatg
     6481 taatgcattt gccaaatact tcaaaaggag aatttgccta taaactttgg aaagatgaaa
     6541 tgataagatt aaaagaagag gttgagaaat ctttaggtgt aactataact gaagaagata
     6601 taagaactgc tataaaagat aaaaatgaag aaagagaact tttaaaagag ttttatgcac
     6661 ttggtaaatt acaaccatct gcacttacag gccttgagtt acataatgtt ttatatcaag
     6721 caggttttaa atttgaccgt gcagaactta aacattcttt aagaaaagtt atagatgata
     6781 tgaaagaaag atatgaaaaa ggtgaatgtc ctgttcaaaa agataagcca agaatattga
     6841 taacaggttc tcctattggt ggaataagcg aaaaaatagt taaaacttta gaagatgcag
     6901 gggcatcagt cgttgcatat gaactttgtg gagctattag atcaaacgat ttattggttg
     6961 atgaagaaat agaagatgta tatgatgctt taacacaaaa atatataaat ataggttgtt
     7021 cttgtatgat gaataatgac aatcgtattg aattattaga tagaataata gatgaatata
     7081 atgtagatgc tgtaatagat gtagttttac aagcatgtca tacatttaat atagaaagtt
     7141 atagaataag agaatttgtt acaaaagaaa aaaataagcc atttatgtct cttgaaactg
     7201 attattcaaa atcagatact gaacaattaa gaactagatt tgaagcgttt gtagagatgt
     7261 tgtaggaata aataaaagta ggaggaacta tgtttagtat aggagttgac tcagggtcag
     7321 tggcaacaaa gggtgtgcta tttgatggtg aaaaaattat taagaaaata attataccaa
     7381 ctggatggag ccctaaaagt acatctaaac aggtctatga gttgctaagt agtgaaattg
     7441 acaaaaaaga tataaaaaag gtggtaggta caggttatgg aaggggagta atggattttg
     7501 ctgataagaa agtaacagaa ataacttgtc atactagagg tatttacttt ttaaacaaga
     7561 acataaggac tattttagat gtaggtggtc aagatagtaa agttataaat ttagatagag
     7621 atggaaatgt atttaacttt ataatgaatg ataagtgtgc tgcaggaact ggtcgatttt
     7681 tagagattac ttctaacctt ttaggaagcg atattgaaag tatagataca ttagctaaag
     7741 gatatgaacc agttaatata tctagtatgt gtacagtttt tgctgagtct gaaattgtaa
     7801 gtcttctagc tcaaaacatt tctactggag aggtagcagc agggatactt aaatctattg
     7861 caaataaatc cacctcaatg ttagcaagag gtgaggtaat agatgaagtt gcatttactg
     7921 gagggcttgc aaaaagtaaa gaactagtaa aaatgataga agaaatactg ggtaaaaaaa
     7981 tttttattgc agaagatact caaataatag gagctttagg agctgctgta ataggattta
     8041 gataaaaaca tataaaagga aaatgagaag ctgaatttat tataaataaa ttcagttttt
     8101 tattttattt tatttattta taaaggaaaa ctttggtata attgatttaa atgattgtat
     8161 atttgtaaat acttgatatt tagtcatata taacattata attttaaaaa ttagctcaaa
     8221 ataaggggga gaaagcgtga aaaaatttac ttcaaaaaaa gtaacaaggt ttgttgcgct
     8281 gtttttaata tctattttag taatgtcaac aattcctgta tcagctgaca atagcaatac
     8341 attgaatcag ataaaaaaag cagaatacag tgagacgtat aaacaatatc taaatgatgc
     8401 taaaaatgga cgtacagaaa aatataatgg gattatacca aatccatatc agttggaagg
     8461 tactcaaatc caaagtaaag gaagaacagc tccaacatct tatgacccta gaaaattagg
     8521 tcttatgaca tcaataaaaa atcaagagga tttaggaatc tgttgggatt ttgctgcaat
     8581 ggcaacctta gaaagtttct taaagttaaa taattatgga gactatgatt tatcagaaga
     8641 acacttaaga tggtgggctt cagatggtga atatggatgg tctgtaaatg atatgaatgg
     8701 tgctttaaat tatgaagcta tgggatattt aacatcttgg tcaggtccta aactagaaaa
     8761 agatattcca tacaatggta gagtatctaa agcacaagga gcaaaaaaac caactaatat
     8821 gaatacagca cctactgttt ttaatgtgac agatgctgtg tgtgtaagta atgacataaa
     8881 ttctacaaaa aatgctatat tacagtatgg agcagtaaca tctggttatt atgaagatat
     8941 taaatatcaa agtgatgacc aaaattcata ttattgtcca acaaaaagtt ttaatacaaa
     9001 tcatgctatt tcaattgtag gttgggatga taactattct aaagataatt ttaatgcaaa
     9061 tattagacca tcaaaaaatg gtgcttggtt aataaaaaat agctggggag attataattc
     9121 tgagggtgga tatttctgga tatcttatga agacaagaca cttatgtctg atatagataa
     9181 tttttcaatc aaaggtggta aaaaaccaaa tgatgatgag aagatgtatc aacatgacta
     9241 tgcaagtatt gtacctttaa tttcaaaaaa gataacagct gcaaatgtat ttgatttcaa
     9301 tagaggcgat gaaactctaa aatctgtaat gttcttaact gaaagtatag gagctaagta
     9361 tgaagtatat tatgctccag tagtaaatgg tatacctcaa gaaaacaata tgaaaaaatt
     9421 aaaagaaggt actgcccaat attcaggata cataacagtt ccaattgatt cttttgatat
     9481 accagaagga aagggtgcaa ttgtagtcag tatagagggg aaaaatggag agtcaactat
     9541 aggttctgag tcaaatgtac caggatatga tacatttaaa gcaaaagcta atttaggtga
     9601 aagttatatc atagataata ctggaaaatt ctttgatata aatagagata gtaacttcta
     9661 tccgtgtaat tttactataa aagctgttac agaaaagtct tctggtgaga gtatcccaaa
     9721 tgagtcttta attggtagtg atagatatga aactgctatc aaagttagtc aaaatggttt
     9781 caattcttca gaaaatgtag ttttagtaaa tggcagttca atagtagatg cattggcagc
     9841 aactcctttt acatctgcta taaattcacc aatattattg acacaaaaag aagctttaaa
     9901 ttcaaaaact aaagctgaga tacagagatt aggtgctaag aaagtatacc taataggtgg
     9961 agaaaattct ataagtaaag aaattgaaca acaattaaaa agcttaaata tatcgataga
    10021 gagaatatca ggaagtgata gatacaagac aagtttatta ttagctcaaa agttaaatgg
    10081 tataaaaaat gtatctcaaa tagcagttgt aaatggagtg aaaggattag ctgatgcaat
    10141 tagtgtaggc gctgcagcag cagaaaataa cataccaatc atacttgcaa atgaaaagag
    10201 cgaattgcaa ggagctgatg aatttctaaa ttcattaaat atagagaaat cttatattat
    10261 tggaggaaca gcatccctat caaacaatct agaaagtaag ttaaaaaatc caactagatt
    10321 atctggtagt agtagagacg aaactaattc aaagataata gataattttt ataaaaaaga
    10381 tactttaaaa aatgcatttg tagttaaaaa tggaatcaag aatcaaaatg atttaataga
    10441 tggattggca gttggtcctt taggagcaaa aactgggtca ccagtaattt tagttggtga
    10501 taaattagct gatagccaaa aagaagttct taaaaataag actttagaaa aagtaactca
    10561 agttggtgga ggagcaaata aaaatgcatt taaagagtta gtaaaattaa aaagtgctaa
    10621 atagagtact taagtattaa catatattag tgtgtattag taacttatct taataaaagc
    10681 tgaaaataat tacttcaaat tattgataaa taagataatc aatgattgta aagttttgtt
    10741 ttcagcttta aatttatgta gtataagttt aaaaacataa aaaagggtta ctttatatag
    10801 aaatatagaa aatcttacat tttatatata aataacccat atttaattaa ctttttatag
    10861 tgttctaata gatattattg aacttaatgg taaatataat gtgtaagttt catttaaagt
    10921 tggattagat tcatctatat atcttgagca aagtgtaatt gataagctat ctgcttctat
    10981 aatttcacca tatagaggac aactgctaaa gtaagaatgt acttcaacat atttatttaa
    11041 gttttcttga acataatcgt aaaaacggtt ttttgtagta gtagccataa aatcaactcc
    11101 tttatttttt attaaagttg aaacttttaa aaagataaat atgattactt ctattagaaa
    11161 ctaagttaaa aaataagcaa atagatatga aaatta
//
LOCUS       DAMJYR010000119        10918 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.124, whole genome shotgun sequence.
ACCESSION   DAMJYR010000119 DAMJYR010000000
VERSION     DAMJYR010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10918)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10918)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10918
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.124"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(11..643)
                     /locus_tag="PC471_003007"
     CDS             complement(11..643)
                     /locus_tag="PC471_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CatB-related O-acetyltransferase"
                     /protein_id="HDF2790117.1"
                     /translation="MTIPSSNKVYPRSNDYQTVYLKNVITRENIEVGDYTIYNDFYND
                     PRDFEKNNVLYHYPINNDKLIIGKFCSIACNAKFLMTSGNHSMKSKSTYTFPIFYEEW
                     KLDINHITDAWDNKGNIIIGNDVWIGYDSIIMSGVNIGDGAIIGTRAVVTNDVPPYSI
                     VGGVPAKIIKKRFNNDTISKLLEIKWWDWPYKKIQSNIKHIQSGNIDKLV"
     gene            complement(715..1068)
                     /locus_tag="PC471_003008"
     CDS             complement(715..1068)
                     /locus_tag="PC471_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase DNA-binding domain-containing protein"
                     /protein_id="HDF2790118.1"
                     /translation="MSTEKRRDNKDRILRTGESQRKDGRYAYKYVYSFGKPQFVYSWK
                     LVPTDKPPKGKRDDISLRDKEKAIQNDLDDSIDTIGKKMTVCQLYAKKNGLRKNIRLN
                     TKKGRHYLMKILNKE"
     gene            complement(1146..1349)
                     /locus_tag="PC471_003009"
     CDS             complement(1146..1349)
                     /locus_tag="PC471_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HDF2790119.1"
                     /translation="MNNTDVPIWEKYTLTIEEASKYFRIGEKKLRKRADENLDSGWVI
                     MNSNRIQIKRKQFEKIVDTLDVI"
     gene            complement(1739..1969)
                     /locus_tag="PC471_003010"
     CDS             complement(1739..1969)
                     /locus_tag="PC471_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF2790120.1"
                     /translation="MKPTKEVLPFTIILLAVHGDVNAINVILKHFEHYIIRLSQKTLF
                     DEYGNPYIHVEEETKRMLGTKLITAILRFNLS"
     gene            complement(1971..2378)
                     /locus_tag="PC471_003011"
     CDS             complement(1971..2378)
                     /locus_tag="PC471_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HDF2790121.1"
                     /translation="MVDEKTIEHQFDSFCKTVLRNYARDIYDENKRRNDYLVSLESLS
                     LAELSKLSILDDYDSNYICMVSYDYNIRIEDVLMAQAIGKLTKRKQDIILLSFFFNMT
                     NADIATLMDLAENTIHYHKTNALKELKELMQEH"
     gene            complement(2738..3187)
                     /locus_tag="PC471_003012"
                     /pseudo
     CDS             complement(2738..3187)
                     /locus_tag="PC471_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729090.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            complement(3201..4127)
                     /locus_tag="PC471_003013"
     CDS             complement(3201..4127)
                     /locus_tag="PC471_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2790122.1"
                     /translation="MSDILSRITNKIGDKNIVDKLSALSKSDLNSLLLEVFDRQANTL
                     TATDILKSYQLNRFTIPSGIDPEEIHALESKLLRKAFNMDIKTIMLSPSAPLGSCSVF
                     GSVDQYNVVSALRGTETLADPSNMLAIIIADKLKRKEENNTIPIHMATTARVIRAQNF
                     VGKGLYSHFGLYCMVSSGIDTGSYTCEKMLLEKHLNYYKDILSEIENVRFSIILRKRN
                     GYKDNDGFFDRMVETIKLIFPNIELSIHNDDVDNNYYKGINFKLSVKQGNETVEIVDG
                     GFVDWTYQMLGNKRERCLISGIGLERFLVAVS"
     gene            complement(4252..5034)
                     /locus_tag="PC471_003014"
     CDS             complement(4252..5034)
                     /locus_tag="PC471_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside 6-adenylyltransferase"
                     /protein_id="HDF2790123.1"
                     /translation="MTKTQQKMIQNVLEFSKRDSRIIGLAISSSYITKSLDEYSDLDF
                     LIVADDKSYEEVIEERLLIVEQFGTLVSAFTGEHVGEPRLIISLYDSPVLHVDFKFTT
                     LDGLLDRVEDSAILYEENNCITEIYSKKEAYFPTPDLQWIEDRFWIWVHYACTKIGRR
                     ELFETIDFLSFLRQSVLAPLIQLLLGKLPRGVRKIELDAPQYIEALEKTVANHEYKSC
                     VNALQATINLYLELREELSQSPIVRKVEAERVALQYFQDLIK"
     gene            complement(5190..5810)
                     /locus_tag="PC471_003015"
     CDS             complement(5190..5810)
                     /locus_tag="PC471_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HDF2790124.1"
                     /translation="MHLIDFMNEDIKKKLVKKTYKKKETILFAEQENEYVILMIDGIA
                     EAFILNVKGNISTIHLYRSGSFFGEMEQFNDGKKPVEIVAFTDCNVYKLHRDDFLNWL
                     RGDFEATKFLIREISSKLVVNAELIEELSSLSVKERLLRCISLHYYRSELNLLTKKQL
                     ASEVNAPIRSINRAINECVQEHLIAYENKRFSVINQREVLKNLPTT"
     gene            complement(5898..6596)
                     /locus_tag="PC471_003016"
     CDS             complement(5898..6596)
                     /locus_tag="PC471_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDF2790125.1"
                     /translation="MYKLSYVVRVSTKNLDELKRRCDEVKDFYDDLSIKLVRPFGDML
                     GLHGEFIPVSKRYINDYIQYVTSDFLAGLGFGATQTLGEHEDIYLGYNLDTGKNVYLK
                     PALASQGVKGSITNALASAFIGSLGGGKLFSNNMLVYYAVLFGGQAVIVDPKAERGKW
                     KETLPEIAHEINIVNLTSNDENKGLLDPYVILSRKKDSESLAIDILTFLTGISSRDSD
                     KFLQKNDNWKIIME"
     gene            6697..7512
                     /locus_tag="PC471_003017"
                     /pseudo
     CDS             6697..7512
                     /locus_tag="PC471_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897377.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
     gene            8184..8951
                     /locus_tag="PC471_003018"
     CDS             8184..8951
                     /locus_tag="PC471_003018"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HDF2790126.1"
                     /translation="MKRKVQVKNITIGEGRPKICVPIIGKNKKDIIKEAKELKDACLD
                     IIEWRVDFFENVENIKEVKEVLYELRSYIHDIPLLFTFRSVVEGGEKLISRDYYTTLN
                     KEISNTGLVDLIDVELFMGDEVIDEVVNFAHKKEVKVIISNHDFNKTPKKEEIVSRLC
                     RMQELGADLPKIAVMPQNEKDVLVLLEATNEMFKIYADRPIITMSMSGMGVISRLCGE
                     IFGSALTFGAAKSVSAPGQISFKELNSVLNLLHKSIN"
     gene            complement(9279..9956)
                     /locus_tag="PC471_003019"
     CDS             complement(9279..9956)
                     /locus_tag="PC471_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2790127.1"
                     /translation="MIVIEGSDKFKIAKEYIDVEYTLFSKVTFRYEKLKFKDNAELEK
                     IKMFKYKNGYIPNKLNLSFGYGFSSYKKQIIRETVDTLRLTEIFSSENIEDIKFIKDG
                     TKKLEISIEKVVKFKRRKKRNYVCCYCPDMYRDIKLDKESINKIYNRKIKIEREVNIF
                     EDEDVIINKKVLKFPKSWTKNMQKYWLSENKYPIHSTVIDDDRYKCCNVQYTKNRVII
                     LYYIYNH"
     gene            complement(10106..10423)
                     /locus_tag="PC471_003020"
     CDS             complement(10106..10423)
                     /locus_tag="PC471_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2790128.1"
                     /translation="MNYIGKRLKEERKKANLTSKEFANMVGVSPWYITQIESGKKNPS
                     LKTFIKFVNILNISADVLIKDITSTGKTYLENDINEELKDLNSRELNLISQIIDIIKK
                     NKI"
     gene            complement(10445..10783)
                     /locus_tag="PC471_003021"
     CDS             complement(10445..10783)
                     /locus_tag="PC471_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2790129.1"
                     /translation="MANIGKIIGDRIKVYRTKLGYSQEFLAEKAGLHPTYIGQIERAE
                     TNTSINIIMKIATALDIPLELLFANIITTEGVDNSVPLECYEMINKLTEKEQIAMLEL
                     IKCIIKYKEI"
BASE COUNT         3607 a         1731 c         1559 g         4021 t
ORIGIN      
        1 ttataaaata ttaaactaat ttatcaatat ttcctgattg aatatgctta atgtttgatt
       61 gaattttttt atatggccaa tcccaccatt taatttctaa taattttgaa attgtatcat
      121 tattaaatct cttttttata atttttgcag gtactcctcc aacaatagaa tacggtggaa
      181 cgtcattagt aactactgct cttgttccaa taattgcacc atcaccaata tttacacctg
      241 acataattat agaatcataa cctatccaca catcatttcc aattataatg ttacccttat
      301 tgtcccaagc gtccgttata tggttgatat cgagtttcca ttcttcataa aagatgggaa
      361 aagtatatgt ggattttgat ttcattgagt gatttccact agtcattaga aattttgcat
      421 tacatgctat tgagcaaaac ttaccaatta ttaatttatc attgtttata gggtaatgat
      481 acaatacatt gtttttctca aaatctcttg ggtcattata aaagtcatta tatattgtgt
      541 aatctccaac ttctatattt tcccttgtta ttacattttt aagatataca gtttgataat
      601 cattacttct tggatatacc ttatttgaac ttggaatagt catatttaaa tctctccttt
      661 aatatatatt atgtttttga ctattccact actacaatac atctcttcac agaatcactc
      721 cttgttaaga atcttcataa gataatggcg tcctttttta gtgttaagtc ggatattctt
      781 tcttagtcca ttctttttag cgtataactg acaaactgtc attttcttac caatagtgtc
      841 aatgctatca tcaaggtcat tttgtattgc tttttcctta tcccttaatg atatatcatc
      901 acgttttccc tttgggggtt tatcagtagg tacaagtttc caagagtaaa caaattgtgg
      961 tttaccaaat gaatatacat acttatatgc gtatcttcca tcttttctct ggctctctcc
     1021 agttcttaaa attctatctt tattatctcg tcttttttca gttgacatga taagaagctc
     1081 ctctcaataa ccgaattgag cttgatacgt catatgaata ttataacata taaggctcaa
     1141 tttcattaga taacgtcaag tgtatcaacg attttctcaa attgttttct ctttatttga
     1201 atgcgattgc tattcataat gacccagcca gaatcaaggt tttcatcagc acgtttacgt
     1261 aattttttct ctccaatacg aaagtatttt gacgcttctt caatcgtcaa ggtatatttt
     1321 tcccaaatag gtacatcagt attgttcata taatgtatca ctcctttcta tgttcgtagt
     1381 tcagaagtag atagatggct ttgataagct ccattggtat ttcaaccaat cccattctta
     1441 tggacagaac cgtgtcatac aggtgtatca ttatactgtg gtacaggtta cggcaaaaca
     1501 accgttccaa aggttattca caaatcacta tatagtagct ctcttgatta aggtcatggc
     1561 gtatagtatt tgttggctat ctgtatcaca aacgtatcta tctactttat tcagttgtca
     1621 aagaacagtc ggcattgatt tgcctataaa aaatcacaat agcttgtggg cttttataga
     1681 caaataatta gtgattctcc atgtaccaaa tagcttgata catggagaga gggcgagatt
     1741 aagacaaatt aaatctcaaa atagcagtga ttagttttgt tcctagcatt cgctttgttt
     1801 cttcttctac atgaatatag ggatttccgt attcatcaaa taaagttttc tgtgaaagac
     1861 gaatgatata gtgttcaaaa tgtttaagta ttacgtttat tgcatttaca tcgccatgta
     1921 cagcaagtaa aataattgta aaaggtaata cttctttagt aggtttcatt tcaatgttcc
     1981 tgcatgagtt ctttcaactc ttttagtgca tttgttttgt gataatgtat ggtattttct
     2041 gctaaatcca tcaatgttgc tatgtcagca tttgtcatgt tgaaaaagaa tgaaagtaga
     2101 attatatctt gttttctttt tgtaagtttt ccgattgctt gcgccattaa tacatcttca
     2161 attcgtatgt tgtagtcgta agaaaccata cagatgtagt tagaatcata atcatctaaa
     2221 atactcaatt tacttaattc tgcaagtgac aaagattcga gagataccaa ataatcattt
     2281 ctgcgtttat tttcgtcata aatatctcta gcatagttac gcaaaactgt tttacagaaa
     2341 ctgtcgaatt gatgttcaat tgtcttttca tcaaccataa gatttcaccc tctttccatc
     2401 gcaaggagtt aaataacctt gcttactcct atcacggtgg gagggcgtta aatcttcgat
     2461 aaatttcaaa aaaaagagaa aaaaatacac actctgccta tagggcaaaa atgtgtattc
     2521 tttaaaacgt atttaattta tattggttgt aagccaccac catattgatg tatggaaata
     2581 tgcattggct agcttttcat aaaaatacaa agatgcataa gaaattaata gaggggaaaa
     2641 acttattctg ttattaaaaa gtaggttaat tctaataaat gctattgcgg tcgaattaaa
     2701 ctatatacat ggcaatcaat catgtttcta ttctttatta tgaattgtga cagtattctt
     2761 caatcttaaa gccacatttt tccaatactt tacttgactc tctattgtag gaatatacat
     2821 tagcatgaat tttgttaagg ttgtattttt caaaggcaat tttgcatatt tcttttactg
     2881 catttgttgc tatccctttg cctcaatagt tcctagcaag ccagtaacct aattcagcat
     2941 ttttattaat agaggtatct tcttcaaatg ttaggctgat acttccaata gccctttctt
     3001 gataaaaaat agcatagcta agttgtttaa catctgaagt tgccaaacaa aaactaacaa
     3061 aatctctagc gttttgctcc ctataagggt gggggaagtt gtcacgaaga ttctttgcaa
     3121 tttctttatc attcgcaaac tcaactaaat cggtaatata agcataattc cactctataa
     3181 gttccatttt tcttctcctt ttatgaaaca gctacaagaa atctttctaa gccaatacca
     3241 cttatcaaac agcgttctct tttatttcca agcatttgat aagtccagtc aacaaatcca
     3301 ccgtcaacta tttcaacggt ttcatttcct tgtttgaccg ataatttgaa attgatacct
     3361 ttataataat tattatcaac atcatcatta tgaattgata actctatatt aggaaaaata
     3421 agtttgatag tttcaaccat tctgtcgaaa aatccgtcat tatctttgta tccgtttctt
     3481 ttgcgtagaa taatagagaa acgaacattt tctatttcac ttaatatatc tttataatag
     3541 tttaagtgct tttctaacaa cattttttca caagtgtaag accctgtatc tataccagat
     3601 gaaaccatac aatataagcc aaaatgtgaa tataaacctt tacctacaaa attttgtgct
     3661 cttattaccc tagcagtagt agccatgtgg ataggtatgg tattattttc ttcttttctt
     3721 tttaatttat cagcaataat tattgccaac atatttgagg ggtctgcaag tgtttctgtc
     3781 cctcgtagag cacttactac gttatattga tctacggagc caaaaacgct acagctacct
     3841 aatggggcag agggagaaag cataatcgtc ttgatatcca tattgaatgc ttttctcaat
     3901 aattttgatt ccaaagcatg aatttcttct ggatctatac cgctagggat agtaaatcta
     3961 tttagttggt atgactttag aatatcagtt gcagttaaag tatttgcttg tctatcaaac
     4021 acttctaata gaagtgagtt caaatctgat tttgatagag ctgataactt atcaactata
     4081 ttcttatccc caattttatt tgtaattcta cttaaaatat cactcatagt tatcctccat
     4141 actgttactt tcaaaataat tatagaattg ttacaacaac taatctaggt catttgacca
     4201 aaaatgaaaa aacactgaat atttactatt caatattttt ttactttggg tttatttgat
     4261 taaatcttga aaatactgta atgcaaccct ttcagcttca acctttcgga caataggact
     4321 ttgtgataat tcctctctta actcaagata caggttgata gtagcctgca atgcattaac
     4381 acagctttta tattcatggt tagcaaccgt cttttctaat gcttcaatat attggggtgc
     4441 gtccaattca atttttctaa ctccacgagg taatttacct aagagaagct gtattaatgg
     4501 ggcaagtact gattgtctta aaaaagataa aaaatcaatt gtttcaaata gttctctacg
     4561 tccaatctta gtgcaagcgt aatgaaccca aatccaaaat ctatcttcaa tccactgtaa
     4621 gtctggagtt gggaagtatg cttctttttt agaataaatt tctgttatgc agttgttttc
     4681 ttcatagaga atagctgaat cttcaaccct atctagtaaa ccatctaagg ttgtaaactt
     4741 aaaatcaaca tgaagaacag gggaatcata gagggaaatt atcaatcgag gttctccaac
     4801 gtgttcgcca gtaaaagcag aaactaatgt tccaaactgt tccacaataa gtaatctctc
     4861 ttcgataact tcttcatagg atttatcatc tgctacaatc agaaaatcca aatcagaata
     4921 ttcatctaac gattttgtga tataagaact gctaattgca agtcctatta tcctactatc
     4981 tctttttgaa aattctaata cgttttgtat cattttctgt tgtgttttag tcataatata
     5041 acacctctca ctgtctttta ttagctatca atccaacata attatgcata ggagtattat
     5101 ataatgaaga aataaaaaaa aataggtcac ttgacccgaa ataaaaaagc attgaataat
     5161 tattattcaa tacttttttg aaattatact tatgttgtag gaagattctt taatacctct
     5221 cgctgattaa ttacactaaa tcttttgttt tcataagcaa ttagatgttc ttgtacacat
     5281 tcatttatag cacgattaat gcttctaatt ggagcgttta cttcgctagc aagctgtttt
     5341 ttggtaagca gatttaattc acttctataa tagtgtaagg atatacacct cagaagtcgt
     5401 tctttaacac ttaaagatga taattcttca attaattcag catttacaac taatttacta
     5461 gatatttctc gaatcagaaa ttttgtagct tcaaaatctc cccttaacca gtttaaaaaa
     5521 tcatctctat gaagtttata tacattacaa tctgtgaaag cgacaatttc aacgggtttt
     5581 ttcccatcat taaattgttc catttctcca aaaaaactac cacttctata aagatgaatc
     5641 gttgagatgt ttcctttcac atttaagatg aatgcttctg caataccgtc aatcataaga
     5701 attacatatt cattttcttg ctcagcaaaa agaatagtct ctttcttttt atacgttttt
     5761 ttaacaagct tctttttaat atcttcattc ataaaatcaa ttaaatgcat attcatccct
     5821 ccttaccaaa tctttgatta aattatatca tctttcaatt agttaggtaa gatagtaaaa
     5881 atatatttac atagaaacta ttccattatt attttccagt tgtcattttt ctgcaaaaac
     5941 ttatcactgt cacgactgga gataccagta aggaatgtta ggatatcaat cgctaaactt
     6001 tcagaatcct ttttacggct caaaatgacg taagggtcaa gcaatccttt gttttcatca
     6061 ttactggtta aattgacaat gtttatctca tgggctattt ctggtagcgt ttctttccat
     6121 ttgccgcgtt ctgctttagg gtcaactatg accgcctgtc cgccgaatag taccgcatag
     6181 taaacaagca tattgttact gaataattta ccaccaccca atgaaccgat aaacgcagac
     6241 gctaacgcat tggtaataga accctttacc ccttgactag caagggcagg ttttagatag
     6301 acattctttc cagtgtccag attgtagcca agatagatat cctcatgttc gccaagggtt
     6361 tgtgtcgctc caaaaccaag tccagccaaa aaatccgaag tgacatattg aatataatca
     6421 ttgatgtaac gtttgctgac aggaataaat tcgccatgca atcccaacat atccccaaag
     6481 ggacgcacga gcttgattga taagtcgtcg taaaagtctt tgacttcatc acaacgcctt
     6541 tttagctcgt caagattctt tgtagataca cggacaacat agctaagttt atacatagat
     6601 tctttacagt taaaagtctg tttttaaaaa aatgtataaa ttataaatca aaatatagat
     6661 actatcaatg acccactagg tgcaaaaagc attgatatgg ttaaattatc agacgctaaa
     6721 agctgggcta tccgaatgag tgaagatggg tgtgcttata aatccattga caattataag
     6781 cgctcactga aagcgtcgtt tttcatggca attcaagacg actgtattag aaagaatcca
     6841 tttaattttt agttaagtga tgttttggaa gatacaacag aaccaaaaat cgctctaaca
     6901 ccagaacagg aaatgggctt attatccttt atggggagcg ataagactta ttgtaaatat
     6961 tatgatgatg ttgttatcct cttagaaaca ggactacgca tttctgaatt atgcggtttg
     7021 actttagaat tggatatgaa aaataaagcc attatggtag accatcaatt gttgagtgat
     7081 actaagattg gctattatgt agaagttcca aagaccacac agggtaagcg agaaattcca
     7141 atgactgaaa gagcttatca atcatttgaa cgtatcgtgg aacggcgtaa gaacgctaaa
     7201 gcagaaccaa tcattgttga tggttaccag aacttcctat ttcttaaaca agatggatta
     7261 ccaaaacaag caactcatta tcaaagcatg ttaagagcgt tagtcaagaa gtataataag
     7321 acccatgagg acaagctacc aaagattacg ccacatacct ttaggcatac actctgtaca
     7381 aatatggcta acaaaggaat gagtcctaat atattgcaat atattatggg gcataaaaac
     7441 attactatga cgcttggcta ttatgcacat ggttcattta gttcttcgag agctgaaatg
     7501 gaacggttat aatcctagta gtatgactag agtttactac ttatttacta cttttgatgg
     7561 cattcttata cggttaaatg ccaacttatg caaagtatct tccaaaaaga aaatgccgat
     7621 actccttgta gatacaggat atatcgacat ttaccaactt atgaaaagat aatcaaattt
     7681 aatataagtt tttcaaataa aaagatacca aattcttttt cgagaatcta tataacatta
     7741 cttaagcttg gttggggtat aaacaattga gtagctactt ttgtatggtg ttcaacttta
     7801 gtcaaaactc ttaagtaatg caaatgattt agattcatta atattcaatc tcttcataag
     7861 tagtgaaatt cgagttattt ctgcataatt atactatgat ttatagatta attctattag
     7921 catccagaat aatattgttt atatgagtaa aatttaaaga atattgtgaa aattatagga
     7981 tgttaaaata aatttaggta aaaaagaagt gtttgtatat tatagagtac cttttctaaa
     8041 atatgtggga ttgttttaag aattagaata aaaattattt ttaaattagt atttttataa
     8101 tgtatagtat ttttaatgta taattaaatt gtgcagaaaa tagaatttaa agtcctatag
     8161 aatttctgat tatggaggag aatatgaaaa gaaaagttca agtaaaaaat attactattg
     8221 gagaaggtag acctaaaatt tgtgtaccaa ttattgggaa aaataaaaaa gatattataa
     8281 aagaggctaa agagttaaaa gatgcatgtt tggatattat agagtggcga gttgattttt
     8341 ttgagaatgt agaaaatatt aaagaagtta aggaagttct ctatgaatta agaagttata
     8401 tacatgatat ccctttatta ttcacattta gaagtgtagt agaaggggga gaaaaattaa
     8461 tatcaagaga ttattatact actttgaata aagaaatttc taatacaggt ttagtagatt
     8521 taatagatgt agagttattt atgggagatg aggtcataga tgaagtagtt aattttgctc
     8581 ataaaaaaga agtgaaagta attatatcta atcatgattt taataaaact ccaaaaaaag
     8641 aagagattgt atctcgttta tgtagaatgc aggaattggg cgcagattta cccaaaatag
     8701 ctgttatgcc gcaaaatgaa aaagatgtgt tagtgctact tgaagctaca aatgaaatgt
     8761 ttaaaattta tgcagataga ccaataataa caatgtcaat gtcaggaatg ggtgttataa
     8821 gtagattgtg tggtgaaata tttggttctg cattgacttt tggagctgca aaaagtgttt
     8881 cagcaccagg gcaaatatca tttaaagagt taaattcagt acttaatcta ttacataaga
     8941 gtattaatta aattaattat atagaaatat tagacggtat ttttgaagat ataaattaaa
     9001 tataaaaaac ttttcagata gtaaaattta aattgggcaa ttatatctga aaagcaacta
     9061 tttagtgtgt attttgaaag tgatatagaa aatcttataa atcaactatg gcatatttaa
     9121 aatatgccat taaaacctac tagacattag attatagatg gtatactagc atttctatta
     9181 tttatagata gtaagttttc ataataactg ttttatctaa agtagaagat atatttgtat
     9241 tatgtatatt tgtcattatt tttttgtaat tatgtatatt aatggttata tatgtaatat
     9301 aatattatca ctctattttt tgtatattgt acattacaac atttatatct atcatcatca
     9361 attacagtag aatgtatggg atacttattt tcacttaacc aatatttttg catattcttt
     9421 gtccaagact ttggaaactt caatactttc ttgtttatta taacatcctc atcttcaaaa
     9481 atattaactt ctctttctat ttttattttt ctgttgtata tcttattgat agattctttg
     9541 tcgagtttta tgtctctata catatcaggg caatagcaac aaacataatt tctttttttt
     9601 cgacgtttaa atttcacaac tttctctatg ctaatttcta atttttttgt accatctttt
     9661 ataaatttaa tatcttctat gttctcgctt gaaaaaattt ctgtcaatct taaagtatct
     9721 acagtttctc taattatttg ctttttataa gaagagaatc catatccaaa agaaaggttt
     9781 aatttattag ggatgtagcc atttttatat ttaaacatct taattttttc caattcagca
     9841 ttatctttaa atttcaactt ttcataccta aaagttactt tgctaaaaag agtatattct
     9901 acatcaatat attcttttgc aatcttaaat ttatcgctac cttcaatcac aatcatagcc
     9961 tatctccaat cgttttatat taaaataagg ccatagaaca tggtctaata gtactcatta
    10021 gcactataag acaatgttaa cacaaaaaga caaaaaaagg ttatttattt caaataacct
    10081 ttttttttat ataaaatata aagaattata ttttattctt ttttatgatg tctataatct
    10141 gtgatatcag atttagttct cttgaattta agtcttttaa ctcttcattt atatcatttt
    10201 ctaaatatgt ttttccagtg gaagtaatat cttttataag aacatctgca gaaatattta
    10261 atatatttac aaattttatg aatgttttaa gacttggatt ctttttacca ctttcgattt
    10321 gagttatata ccaaggcgat actccaacca tatttgcaaa ttcttttgaa gttaaatttg
    10381 cttttttccg ttcttcttta agtcttttac ctatataatt cattattgtt actatttccc
    10441 tcacttatat ttctttgtat ttaatgatgc actttattaa ctctagcatt gcaatttgtt
    10501 ctttttcagt taatttatta atcatttcat aacattctaa aggtacagag ttatcaacgc
    10561 cttctgttgt aataatattt gcaaataaca attcaagtgg tatgtctaaa gcagtagcaa
    10621 ttttcattat aatattgata gatgtatttg tctcagctct ttcaatctga ccaatatatg
    10681 ttggatgtaa acctgccttt tctgctagaa actcttgact atatcccaat tttgttctat
    10741 agacttttat tctatctcct attatttttc ctatatttgc caattattat ccctccactt
    10801 tagattatat tcatagttta agttgtttta tatagactat ttatactttt ttagtctaaa
    10861 tatagactat atatgttttt atactgaaat aagtgctatt tatttttgta tagtctgt
//
LOCUS       DAMJYR010000120        10842 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.125, whole genome shotgun sequence.
ACCESSION   DAMJYR010000120 DAMJYR010000000
VERSION     DAMJYR010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10842)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10842)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10842
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.125"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(249..1298)
                     /locus_tag="PC471_003022"
     CDS             complement(249..1298)
                     /locus_tag="PC471_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2790130.1"
                     /translation="MVMQVQEKILELLYTINEDVVSIKNDMSEVKQEINVLKEDMSEV
                     KQEMTVMKEDMSEVKQEINVLKEDMSEMKQEMTVMKEDMSEVKQEINVLKGDMSEVKQ
                     EMIVMKEDMSEVRQEINIMKEDMSEVRQEINTMKEDMSEVRQEMTVMKEDMSEVKQEI
                     NVLKEDMSEVRQEINIMKEDMSEVRQEMTVMKEDTSEVKQEINVMKKDMSEVRQEITV
                     MKEDVSEVKSEVNFMQNKINNINEDMNGIKDEVSIANEKLYGIEIKIDSLECEDKSMK
                     EIQIEQNNILGSLLHNSEINKATHDNIEHNIAYIKGDTNSIKEDIAEIRRDLNLVELA
                     TSKNWSDIVKLKSVK"
     gene            complement(1528..2613)
                     /locus_tag="PC471_003023"
     CDS             complement(1528..2613)
                     /locus_tag="PC471_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HDF2790131.1"
                     /translation="MYNFLGKELKSPLIIGSGPLTYSAAGCKILSDAGAGAVVTKTIR
                     KERAINPAPHMVRNTANALLNNEKWTDFEPEQWIDFEIPQMKRDGTVCIASIGHTIEE
                     SSELVEKVANAGADFIELVSYDYRDLIPMLKDAKERVNIPVIVKLPPMIDEIGDFAKK
                     LEEAGADAITACDSVGPAFRIDIETGQPLLGGNGIGYLSGETIKPITLQRIYEIKKQV
                     NIPIIGLGGCVSGDDALEMIMAGADFVGICSVVILKGAQVISKIHDDLKSNLNRLGYN
                     TIENACGIVHNHMGDVNERLSFEFTYDEEKCTKCKMCERVCAYQARKLNDKMELNEEE
                     CRYCGLCISVCKPKALGRKISCSELNV"
     gene            complement(2649..3962)
                     /locus_tag="PC471_003024"
     CDS             complement(2649..3962)
                     /locus_tag="PC471_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HDF2790132.1"
                     /translation="MIAIKSKYLISSADEIYTNSAVVVENDIIKDILPNEEVEIKYKN
                     IEEIIDKSDAIIMPGFVNGHMHQYGVLSRGIPANVHFTDFEGFLNDYWWPFIENRIGL
                     KEVKATTKASAIELIESGVVAFCDTLEAPNTEEGTLIEQGKILEEIGMKAILSLESCE
                     RISFENGLRCLDENSNLIKWSRENSKLINGIMCTHTSFTCSDRFIKKAKEDAKKLNAP
                     MQFHLCESIYEPNYAEKHFGKKAVDYYNDLDILDETVLASQCVKVNDEEIDILKEKGV
                     KVVHMPLSNCEVGGGFSPVPKMIKKGIKVALGTDGYINDFFEVMRGAFLMHKSVEEDA
                     SVMPANLVFRMATEHGAYVLGLQNSGKIAVGNKADIIVMEDEFKTPVTLDNIFDQIVV
                     QGKKEFISNVYIDGRHILKEKQLVDLDKKAIVKEMKEVACEFWKF"
     gene            complement(3963..6254)
                     /locus_tag="PC471_003025"
     CDS             complement(3963..6254)
                     /locus_tag="PC471_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HDF2790133.1"
                     /translation="MVNKEVRKIDSEAIVTGKPIYTEDLIFHKDVLTIKLLRSPHAFA
                     KIKNIDIKNALKVKGVVNIFTYKDVPNYRYSMVGESYPEASPHDQLILEDVVRYVGDE
                     VAIIAAEDERAAIKAMKLIKVEYEVMTPILDARESEGNKILIHEEDDLFCPFDFGLDA
                     TKNIVSREKMGKGDVEKEFKESDVIVEHSYMTQSQAHCMMETHRAYTYIDTNGRLVVT
                     SANQSAYHMRRQVSKALGIPLSKVRVIKPRIGGGFGGKNIAVTEIYASFVTWMTKRPA
                     KLIYTREETFAMTNTRHQMFFDIKIGADKEGNIKAIDMKALNNTGAYGDNGPSVCSES
                     GHNVLPTYNNVPAIKFDGKTVYTNLVPGGALRGYGATQGTFALDCAIDELAHKLNMDP
                     TEIKLKNIIKKQTEGGIMNFPIRSCALDECIIRGKKLIGWDEKYPVRDIGNNKVRAVG
                     MSVGTHGSGIANIDMAIVNMRMDEDGTYKLFSGSSDLGTGSDTILAQIAAKALNTSID
                     RISVYTGDTDMCPYDTGAYASCTTYVTGNATIKSAESLKQKILKVAQKKLDLPIDDLI
                     LYEDRVCYSEDVEKFVLLSELGKESVGGGNQEVLYSSESYGIQESAKPFLAGFAEIEL
                     DKTTGEFKVINYACSTDCGTVMNPALARIQVEGGVTQGIGLAMYEDPRHGSDGKLQTS
                     NLLQYKVPTKKDIGNIIVDFVDNYEPTGPYGAKSLGEIVIHTPAPAIANAIYNATGVR
                     IRELPITSEKVYMAMKKLNQGGK"
     gene            complement(6244..7038)
                     /locus_tag="PC471_003026"
     CDS             complement(6244..7038)
                     /locus_tag="PC471_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HDF2790134.1"
                     /translation="MITIKEYVVPKSLDEAYELLISRKNNIILGGCGFLKLGSKNIGS
                     AIDLKDLALDYINETDDSILIGADTSLRTLELNKVIKNYCNGVISNAVSNIVGVQFRS
                     GARVGASVFAKYGFSDLIPSLLVVDAKVKLYKKGVMNLADFLENELEKDILIEVILPK
                     KDAIGVFDSIRKCTGDFAVLNGAMLKEGSIYKIAIGARPRRAKIAYKASEILSLENDI
                     EKAGEVSSEELSFGSNIRGSKEYRKDMTKALVVRMYNSIGGECDGK"
     gene            complement(7077..7523)
                     /locus_tag="PC471_003027"
     CDS             complement(7077..7523)
                     /locus_tag="PC471_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster binding
                     domain-containing protein"
                     /protein_id="HDF2790135.1"
                     /translation="MIIKCTINNKEKELSIRPDDFLADTLRENGYVSVKKGCDTGSCG
                     LCTVMVNDKAVLSCNYLTARVEGQHITTLEGVLDEAKKFGDFLADEGADQCGFCAPGF
                     IMMVLAMKRELQNPTEEEVIDYVNGNLCRCTGYKGQLRAIHKYLNT"
     gene            complement(7595..9001)
                     /locus_tag="PC471_003028"
     CDS             complement(7595..9001)
                     /locus_tag="PC471_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HDF2790136.1"
                     /translation="MMDILIKNAIIVTVNKEREVIFDGALVVKDNKIADIGNSKEIES
                     KYTDVKKIIDAKGKVLFPGFINTHNHLFQTLLKGLGDDMVLKDWLETMTFPAANYLEP
                     KDTYDAAMLGCIEGLRSGITTMVDYMYPHSKPGLCDGIIDAYKELGIRGILGRGCMNT
                     GAQFGVHPGIMQDVETVEKDVRRLFEKHHNTENGRIKIGVAPAAIWSNSQEMLEMLWR
                     VVKEYDDALFTVHISETPFDREAAKELHGQYDIDVLEKLGILGPNVLMVHCVYLTEKD
                     MELTKKYDMKVSHNTASNMYLSSGVAPVPEMLKKGITVSLGVDGAASNNSQDMLELMK
                     LTALQHKVNKCDPLAMSAEKVLELATIDGARAIGMEDEIGSLEIGKKADLLIFNPMLS
                     PKAIPMHNPVSTLVYSSSMKNIESVIVDGNIIMEDSKILTANEEKALKDAQDTAERLC
                     IRGTIKNRMEGHKWNSLY"
     gene            complement(9019..10389)
                     /gene="hydA"
                     /locus_tag="PC471_003029"
     CDS             complement(9019..10389)
                     /gene="hydA"
                     /locus_tag="PC471_003029"
                     /EC_number="3.5.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropyrimidinase"
                     /protein_id="HDF2790137.1"
                     /translation="MGTILRGGTIITSDKTYISDLRIEDEKIVEIGNNLEINGDKVID
                     ATGKIVIPGGIDTHTHFDMNAGSITTADNFETGTRAAIAGGTTTILDFAEANEGENLL
                     QGVEAYHKKASGNCYCDYGFHMTITCLNKDTFNHMEKLISDGIVSFKMYMAYDGMKVD
                     DGTIYRVLKKARDLGCIVGFHCENGDLLDVLIEENIQNGNLEPKYHPLTRPNIVEKES
                     VSRLADITKLSNSKSYVVHLSCRESLETVKIAREKNIDMIVETCPQYLLLEDNLYNKD
                     KFEGAKYVMSPPLRKKEDINYLWEGLSSGDIQTVGTDHCSFNFKGQKDLGIDDFSKIP
                     NGAPGVEHRLALLYTYGVLENRISVNKFVEVTSTNAAKIFGMYPKKGEIAVGSDADIV
                     ILNINKEETISYKTQKQNVDYTPYEGFKVKCKVEDVFLRGNHVVQSCNVKEHPTGQYI
                     KRKITK"
     gene            complement(10522..>10842)
                     /locus_tag="PC471_003030"
     CDS             complement(10522..>10842)
                     /locus_tag="PC471_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HDF2790138.1"
                     /translation="VEKADCYTGNTIESERFFPGWLYDEEDEFVQAAYKGLKEAGIDS
                     EITQYSFCTNGSHYAGEAGIKTIGFGPSKENLAHTIDEYIEQEQLFIGTEGYYGILKS
                     VYGK"
BASE COUNT         3145 a         2190 c         1130 g         4377 t
ORIGIN      
        1 ttatgttctc attgataaaa ttttacttat ttcaagcgtt ctattagatt ttatcatcta
       61 aattttaggt tttattttta taaattaatt gtatttacat aaaaaaaaag acctaaaaac
      121 aaatttttaa cggtcttttc tcaatttttt attttcattt ctaaaattta taataaatag
      181 taagaacact ataaattctt ttatataaaa ttcttagttt gtggatgata aattagtgaa
      241 ttaaaatact actttactga ttttagtttg acaatatcac tccaattttt actagtggct
      301 aactctacta aatttaagtc tcttcttatt tcagctatat cctcttttat agaattagtg
      361 tcacctttta tatatgctat gttatgttct atattatcat gagtagcttt atttatttca
      421 gaattatgta ataaacttcc taaaatatta ttctgttcta tctgtatctc tttcattgat
      481 ttatcttcac attctagact atcaatctta atttcaattc catataattt ttcatttgct
      541 atggatacct cgtccttaat tccattcata tcttcattaa tgttatttat tttattttgc
      601 ataaagttta cttctgattt aacttcggaa acatcttctt tcattacagt tatttcttgt
      661 ctcacttctg acatgtcttt tttcattaca tttatctctt gttttacctc tgatgtatct
      721 tctttcatta cggtcatttc ttgtcttact tcggacatat cttctttcat tatatttatc
      781 tcttgtctta cttcggacat atcttctttt aatacattta tctcctgttt tacttccgac
      841 atatcttctt tcattacagt catttcttgt cttacttccg acatatcctc tttcattgta
      901 tttatctctt gtcttacctc ggacatatcc tctttcatta tatttatctc ttgtcttacc
      961 tcggacatat cttctttcat tacaatcatt tcctgtttta cttctgacat atctcctttt
     1021 aatacattta tttcctgttt tacttccgac atatcttctt tcattacagt catttcttgt
     1081 ttcatttccg acatatcttc ttttaataca tttatttcct gttttacttc cgacatgtct
     1141 tctttcatta cagtcatttc ttgttttact tccgacatat cttcttttaa cacatttatt
     1201 tcttgtttta cttccgacat atcatttttt atgctaacta catcttcatt tatagtatat
     1261 aataactcta aaatcttctc ttgcacttgc atcaccatcc ctattaatta ttataacatt
     1321 ttacacaaat gctatacaat gttaacatat atatcagaat tattaaaatt ttaattaaac
     1381 aaaattgatt cactttatta cttcaaaact aattaatatc taaaataact acaaataaac
     1441 aaaaataagg tcaacaacaa aaaaggctta tctctttgag atagcctttc ttatttttat
     1501 ttaaaacttt gttttttata aaataatcta tacattcagc tctgaacatg aaatttttct
     1561 tccaagtgct tttggtttac aaactgatat acaaagtcca caatatctac actcttcttc
     1621 attcaattcc atcttatcat tcaattttct cgcttgatat gcacaaactc tctcgcacat
     1681 cttacattta gtacattttt cttcatcata agtaaactca aagctaagtc tttcattaac
     1741 atctcccata tgattatgta ctataccaca agcattttct atagtattat atcctaatct
     1801 attaagatta gattttaaat catcatgtat tttagatatt acttgtgcac cttttaaaat
     1861 tactactgaa catataccaa caaagtcagc acctgccata atcatttcta aggcatcatc
     1921 tccacttaca catccaccta agccaattat tggtatatta acttgttttt ttatttcata
     1981 tattctctgt aaagttattg gcttgatagt ttctccactt aaatatccta ttccatttcc
     2041 acctaataaa ggttgccctg tttctatgtc tattctaaat gctggtccta cagagtcaca
     2101 agcagttatt gcatctgctc ctgcttcttc taatttttta gcaaaatcgc ctatctcatc
     2161 tatcatagga ggaagtttaa ctattacagg tatatttact ctttcctttg catctttaag
     2221 cattggtatc aaatctctat agtcataaga tactaattca ataaagtcag ctcctgcatt
     2281 tgcaactttc tctactaatt cgctactttc ttcaattgta tgacctattg aagctataca
     2341 gacagtccca tctcttttca tttgtggtat ttcaaaatct atccattgct ctggctcaaa
     2401 atctgtccat ttttcattat ttaaaagagc attagctgta tttctaacca tatgtggtgc
     2461 tggatttata gctctttctt ttcttatagt cttagtcaca actgcccctg ctcctgcatc
     2521 acttaatatc ttgcatccag ctgcactata tgttaatggt cctgaaccta ttataagtgg
     2581 tgattttaat tctttaccta aaaaattata cattatctgc ttcccctttt catatatatt
     2641 tacattcgtt aaaatttcca aaattcacaa gcaacttctt tcatttcttt aacaatagct
     2701 tttttatcta aatcaactaa ctgtttttct tttaaaatat gtctaccatc tatgtataca
     2761 tttgatataa attctttttt accctgaact actatctggt caaatatatt atcaagagtc
     2821 acaggagttt taaattcatc ttccataact attatatcag ccttattacc aactgctatt
     2881 ttaccagaat tttgaagtcc taatacataa gcaccatgct ctgttgccat tctaaatact
     2941 aaatttgctg gcattactga tgcatcttct tcaactgatt tatgcattaa aaatgcacct
     3001 ctcataactt caaaaaaatc atttatatat ccatcagtac ccagtgccac ttttatgcct
     3061 tttttaatca tttttggtac tggagaaaaa cctccaccta cttcacagtt actaagaggc
     3121 atatgaacca cttttacgcc tttttctttt aatatatcta tttcttcatc atttactttt
     3181 acacactgag atgctaatac agtttcatcc aatatatcta aatcattata gtagtcaact
     3241 gcttttttgc caaagtgttt ttcagcatag tttggctcat atatactctc acataaatga
     3301 aattgcattg gtgcatttaa tttttttgca tcttctttag ctttcttaat aaatctatca
     3361 gaacatgtga agcttgtatg tgtacacatt attccattta ttaatttact attttctcta
     3421 ctccacttta ttaaattaga attttcatct aaacatctaa gtccattttc aaagcttatt
     3481 ctttcacaac tttctaagga taaaatcgct ttcatgccta tttcttctag tatttttcct
     3541 tgctcaatta aagttccttc ttctgtattt ggagcttcta gggtatcaca aaaagctacc
     3601 acaccagatt ctataagttc tattgctgat gctttagttg ttgccttcac ttcttttaat
     3661 ccaattctat tttctataaa tggccaccaa tagtcattta aaaaaccttc aaagtcagta
     3721 aaatgtacat ttgccggtat tcccctagaa agaactccat attgatgcat atgaccattg
     3781 acgaatcctg gcattattat agcatctgat ttatctatta tttcttcaat atttttatat
     3841 tttatttcaa cttcttcatt tggcaatata tcttttataa tgtcattttc tactacaaca
     3901 gcagaatttg tatatatttc atcagcagaa gatattaagt atttactttt aatagcaatc
     3961 atttatttac ctccctgatt taattttttc atggccatat aaactttctc agaagttata
     4021 ggaagttctc ttatacgaac tcctgtggca ttgtatattg catttgctat tgctggtgca
     4081 ggtgtatgaa taactatttc tcctaaagat ttagctccat atggacctgt tggctcataa
     4141 ttatctacaa aatcaacaat tatgtttcct atatcttttt ttgttggaac tttgtattgt
     4201 aatagattag aagtttgaag ctttccatca gaaccatgtc tagggtcttc atacattgca
     4261 agccctatac cttgagttac accaccttca acttgtattc tagcaagtgc aggattcata
     4321 actgtaccac aatctgtaga acaagcataa tttattactt taaactcccc tgttgtttta
     4381 tctaactcta tttcagcaaa tcctgctaag aatggtttag cactctcttg tataccataa
     4441 gattcagatg aatataaaac ttcttgattt ccaccaccaa cagattcttt tcctaattca
     4501 cttaacaaaa caaacttctc tacatcttca ctataacaaa ctctgtcttc atatagtatt
     4561 aaatcatcta ttggtaaatc gagtttcttc tgtgctactt ttaatatctt ttgttttaaa
     4621 ctttcagcag attttattgt agcatttcca gttacataag tagtacaaga agcgtatgca
     4681 ccagtatcat atggacacat atctgtatct cctgtataaa cggaaattct atcaatagat
     4741 gtattcaatg cttttgcagc tatttgtgca agtattgtgt cagaaccagt tcctaagtct
     4801 gaagacccag aaaacaactt ataagttcca tcttcatcca tcctcatatt tactattgcc
     4861 atgtctatgt tggctatacc agaaccatga gttcccacag acattccaac agccctcacc
     4921 ttattgtttc caatatccct aacagggtac ttttcatccc atccaataag ttttttacct
     4981 ctaattatac attcatcaag tgcacaactt cttattggaa aattcattat tccgccttca
     5041 gtttgttttt ttataatatt ctttaacttt atttctgttg ggtccatatt aagtttatgc
     5101 gctaattcat caattgcaca atcaagtgca aatgtacctt gtgttgctcc atatcctctt
     5161 aatgctcctc ctggaactaa attagtatat acagttttcc catcaaattt tattgcaggt
     5221 acattattat atgttggaag tacattatga cctgattcag agcaaactga aggtccatta
     5281 tcgccataag cgcctgtatt gttcaatgct ttcatatcta tagcttttat gtttccttct
     5341 ttatctgccc caatttttat atcaaaaaac atctgatgtc tagtattagt cattgcaaaa
     5401 gtttcttctc tagtataaat tagttttgct ggtctttttg tcatccaagt tacaaatgat
     5461 gcatatatct ctgttacagc tatgtttttt ccaccaaaac ctccacctat tcttggtttt
     5521 attactctca cttttgaaag aggtattccc agtgctttag aaacctgtct tctcatatga
     5581 taagctgatt ggtttgcaga agttactact aatctgccat tcgtatctat atacgtataa
     5641 gctctatgag tttccatcat acaatgggct tgtgattgag tcatataaga atgttctact
     5701 attacatcac tttctttaaa ctctttttct acatcccctt tacccatttt ttctctcgat
     5761 acaatatttt ttgtagcatc caatccaaaa tcaaatggac aaaacaaatc atcttcttca
     5821 tggattaaaa ttttatttcc ttccgattct cttgcatcca atataggagt cattacttca
     5881 tattcaactt ttataagttt catagcttta atagcagccc tttcatcttc agcggctatt
     5941 atagcaactt catctcctac atatctaact acgtcttcaa gtattaattg gtcatgaggt
     6001 gatgcttctg gatatgactc tccaaccatt gaatatctat aatttggaac atctttgtaa
     6061 gtgaatatgt ttactacacc ttttaccttt aatgcatttt ttatatctat gttttttatc
     6121 ttcgcaaaag catgtggact tctaagaagc ttaattgtta aaacatcttt atgaaaaatt
     6181 aaatcttctg tatatattgg ttttccagta acaatggctt cactatctat ttttcttacc
     6241 tctttattta ccatcacact cacctccaat actattatac attctcacta ctaaggcttt
     6301 tgtcatatct tttctgtatt ctttacttcc tcttatatta cttccaaatg aaagttcttc
     6361 acttgatact tcaccagctt tttctatatc attttctaaa cttaatattt cacttgcttt
     6421 ataggcaatt tttgctcttc ttggtctagc gcctatcgca attttataaa tacttccttc
     6481 ttttaacatt gctccattta atactgcaaa atcacctgta cattttctta tagaatcaaa
     6541 tacacctata gcatcttttt taggtaatat aacttctatt aatatatctt tttcaagctc
     6601 attttctaaa aaatcagcta agttcataac ccctttttta tataacttaa cctttgcatc
     6661 taccactagt aatgaaggta ttaaatctga aaatccatac ttagcaaata cgctagctcc
     6721 tactctagct ccacttctaa attgaactcc tactatatta gaaacagcgt ttgaaattac
     6781 cccattacag taatttttaa taaccttatt taattctaaa gttcttaaac ttgtatctgc
     6841 tccaattaat atgctatcat cagtttcatt tatataatct aaagctaagt cttttaaatc
     6901 tattgctgaa ccaatatttt tactacctag ttttaaaaat ccacatcctc caagtattat
     6961 gttgtttttt cttgatatta gcagttcata agcttcatct aaactttttg gaactacata
     7021 ttcctttata gttatcatac tctctcccca tatcaactta attttcactt aaaagtctaa
     7081 gtgtttaaat atttatgtat tgctcttaat tgacctttat atcctgtaca tctacaaagg
     7141 ttaccattta cataatctat aacttcttct tctgttggat tttgaagctc tcttttcatt
     7201 gccaagacca tcattataaa tccaggagca caaaatccac actggtcagc cccttcgtct
     7261 gcaagaaaat ctccaaactt ctttgcttca tccaagacac cctcaagtgt tgtaatatgc
     7321 tgaccttcta ctctagcagt aagataatta catgaaagca cggccttgtc atttaccatc
     7381 actgtacata acccacatga gcctgtatca cacccttttt taacacttac atatccattt
     7441 tctcttaatg tatctgctaa aaaatcatct ggtcttatac ttaattcttt ttctttatta
     7501 tttatggtac atttgataat caaataaatc acctcaaaac tcatattttt aagctaaagt
     7561 tttactatac ttttttatga ataaattaca tttattaata aaggctattc catttatgac
     7621 cttccattct attttttata gttcctctta tacaaagtct ctcagctgta tcttgagcat
     7681 cttttaatgc tttctcttca ttagctgtca atatttttga atcttccatt attatatttc
     7741 catctactat aacactctct atatttttca tactagaaga atatactaat gtagataccg
     7801 gattatgcat aggtatcgct tttggtgaaa gcattggatt aaatattagt aagtctgctt
     7861 ttttacctat ctccaatgaa cctatctcat cttccattcc aatagctctt gctccatcta
     7921 tagttgccaa ctcaagaact ttttctgccg acattgctaa tgggtcacac ttattaactt
     7981 tatgttgaag tgctgttaat ttcataagtt caagcatatc ttgagagtta ttactagcgg
     8041 ctccatctac tcctaaacta actgttattc cttttttaag catttctggc actggtgcaa
     8101 cacctgaaga taagtacata ttacttgctg tattatgaga cactttcata tcgtattttt
     8161 tagtaagttc catatctttt tcagttagat acacacaatg taccataagc acatttggtc
     8221 caagtattcc taatttttct aaaacatcta tgtcatattg cccatgcaat tcttttgctg
     8281 cttctctatc aaaaggagtc tctgatatat gtactgtaaa taaagcatca tcatattctt
     8341 ttactactct ccatagcatt tcaagcattt cttgagaatt tgaccatatt gctgcaggag
     8401 ctactcctat tttgattctt ccattttctg tattatggtg tttttcaaac aatcttctta
     8461 catctttttc aaccgtttct acatcttgca ttattccagg atgaactcca aattgagctc
     8521 ctgtattcat acatcctctt ccaagtattc ctcttattcc taattcttta taagcatcta
     8581 ttattccatc acaaagacct ggcttactat gaggatacat gtagtcaacc attgttgtta
     8641 taccacttct aagtccttca atacatccaa gcattgcagc atcataagta tctttgggct
     8701 ctaaatagtt agctgctgga aatgtcatag tttctaacca gtctttaagt accatatcat
     8761 ctcccaatcc ttttaataaa gtttggaata aatgattatg tgtatttata aaacctggga
     8821 ataaaacttt acctttagca tctataattt ttttaacatc agtatattta ctttctatct
     8881 ctttagaatt tccaatatca gcaatcttgt tatcttttac tactaatgcc ccatcaaaaa
     8941 taacttctct ctctttattc acagttacta ttatggcatt cttaataagt atatccatca
     9001 ttcttatttc cccccgcatt atttagtaat ttttctttta atatattgac ctgttggatg
     9061 ttctttaaca ttacaacttt gtactacatg atttcctctc aaaaatacat cttcaacttt
     9121 acatttaacc ttaaatcctt catatggagt ataatctaca ttttgttttt gagttttata
     9181 actaattgtt tcttctttat tgatatttaa aatgactata tctgcatcac ttcctacagc
     9241 tatctcacct tttttaggat acattccaaa tatttttgca gcattcgtag aagtaacttc
     9301 cacaaactta tttacactta ttctattttc taagacccca tatgtatata aaagtgctaa
     9361 cctatgttca acaccaggtg ctccatttgg tatcttactg aaatcatcaa ttcccaagtc
     9421 tttttgcccc ttaaaattaa atgaacagtg gtctgtgcca actgtttgaa tatctccact
     9481 agacaatcct tcccaaaggt aatttatgtc ttcttttttt cttaaaggag gagacattac
     9541 atacttagca ccctcaaatt tatccttatt atataaattg tcttctaata atagatactg
     9601 tgggcatgtt tctactatca tatctatatt tttttctcta gctattttta cagtttcaag
     9661 tgactcccta caacttaagt ggactacata ggattttgaa ttagataatt tagttatatc
     9721 agcaagtctt gaaacagact ctttctcaac tatgttaggt cttgtaagtg gatgatattt
     9781 tggctccaaa ttaccatttt gtatattttc ttctatcaat acatctagta aatccccatt
     9841 ttcacaatga aatccaacta tacaacctaa atcccttgct ttctttaaaa ccctgtatat
     9901 tgttccatca tctactttca tgccatcata tgccatatac atcttaaaag atactatccc
     9961 atctgaaata agtttttcca tatgattaaa tgtatcttta tttaaacatg taattgtcat
    10021 atgaaaacca taatcacaat aacaatttcc acttgctttt ttatgatatg cttctacacc
    10081 ttgcagtaga ttttccccct cattggcttc tgcaaaatct agtattgtag tagttcctcc
    10141 agctatagct gctcttgttc ctgtttcaaa attatctgca gttgtaatag aacctgcatt
    10201 catatcaaaa tgagtatgtg tgtctattcc accaggaatt acaattttac ctgtagcatc
    10261 aataacctta tcaccattta tttctaagtt atttcctatt tcaactatct tttcatcttc
    10321 tatccttaaa tcacttatat aagtcttatc agaggtaatt atagttccac ctcttagtat
    10381 tgttcccata tatacctcct aacttttaag ctttaaagta ttttctaagt attgtaaaac
    10441 tttatttaaa attgtatttt ctaaaggtgc tatattcagc acctctagac tatatttaaa
    10501 taatttattg tgaactaaat attattttcc gtatacagat tttaatattc catagtaacc
    10561 ttctgttcct ataaataact gttcttgctc tatatactca tctattgtgt gagcaagatt
    10621 ttccttagaa ggaccaaatc ctatagtttt tattccagct tcacctgcat agtgacttcc
    10681 attcgtacag aatgagtatt gagttatttc tgaatctatt ccagcttctt ttaatccctt
    10741 atacgctgct tgaacaaatt catcttcttc gtcatataac caacctggga agaatctttc
    10801 actttctata gtatttcctg tatagcaatc agctttttca ac
//
LOCUS       DAMJYR010000121        10668 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.126, whole genome shotgun sequence.
ACCESSION   DAMJYR010000121 DAMJYR010000000
VERSION     DAMJYR010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10668)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10668)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10668
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.126"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            150..1310
                     /locus_tag="PC471_003031"
     CDS             150..1310
                     /locus_tag="PC471_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF819 family protein"
                     /protein_id="HDF2790139.1"
                     /translation="MIETGFVYCSFLVVFVGVIFFMVDKLKWKVFDVINPMLLIYLGS
                     AILGSFGLFAQNEDVGTYQNIITVNFLPLMLVFLLIQCDLRKILKMGPKMLLSFFCAT
                     FTFMLGFVIAFIIFKNSMGADSWKILGAASGSWIGGSSNMIAAANALGVAEEGLSYAI
                     LMGSIGYTVWMSVCLMSVKFGPKFNKWTKCDTSFIDEVTSKLEKENKDNTAPTFVELM
                     ILLSVGFGVASFAQIVSKILPTSGILNETMWVVLVASTVSILLGMTKISKLKGSTLVG
                     NVFMYLLLAATGSKANFFGLSEAPVYIMFGLVVVLVHAISFIGLAKLFKLDLFTCEVG
                     SIANIGGVTTAPMIAGVHNPVLIPVGVLMGLLGNIIGTYCALMVTQILHMLA"
     gene            1652..1813
                     /locus_tag="PC471_003032"
     CDS             1652..1813
                     /locus_tag="PC471_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2790140.1"
                     /translation="MIIPSRRIQDITLTNLKNGEVTLIELDEIYKKMGFLFVVSEGKL
                     KKIKKENHH"
     gene            2006..2635
                     /locus_tag="PC471_003033"
     CDS             2006..2635
                     /locus_tag="PC471_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HDF2790141.1"
                     /translation="MKIVTYNIHKGMDSNNRLTLTKMGLYLKQLDCDVICLQEVLYPQ
                     FLALKAVLNMDGVFATNVKKVNMIYGICTFTTSKMLNSNHFFLTSKKEQRGALCITID
                     AYGRIINVINTHLGLDRQERAKQLDEIIDYRNRLVGIVILCGDFNEKNVFLSMFNDMA
                     ISLNKSYLPTFEKSNSRIDYIFVNKNTELKGYTVEKIYLSDHYPVIGYI"
     gene            2874..4133
                     /locus_tag="PC471_003034"
     CDS             2874..4133
                     /locus_tag="PC471_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HDF2790142.1"
                     /translation="MGRRTTRVSRLGKEKKRSVLIVLAALVLIMGGFTYFFNSKFLYN
                     GKIAKNVYIEGVNVSDMTKAEALKAITDKYTPEDLNLAYDGKKYTISPKDIDLKYDTE
                     DVVKDAYESTKKGSYFQNLKKYIDIRVNKANMKIKAEYDEAKLSSKVSSIADSINVKM
                     KNASISVGSGGLSYTDSVVGREFDLAANKESIYNMIKNKEHKTLELKVNLQKPDITTE
                     QVKTVNSVIGQYSTTYSQAVEGRSYNVGLSARKTSDVLLMPGEEFSYNKLTGPSNKAN
                     GYKDAPVIVYGKLEQSAGGGVCQTSSTVYNAALLSGMEITQVTNHSSASTYVPKGRDA
                     TVSDGGLNLKFKNPYKHPVYIKNYAGGGSVSSVIYGNSGDKPNISIEVKQTGQNKYST
                     YRIFKDSSGKVIKKEHISNSSYKELKK"
     gene            4392..5387
                     /locus_tag="PC471_003035"
     CDS             4392..5387
                     /locus_tag="PC471_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HDF2790143.1"
                     /translation="MYEEVTWLNFIGNRYEVVNSDDVLEINKIYKARDVFYRKNVLIK
                     VIKHNNYICEDFVSNLIDESTALDEINSPYILKIIDVGIHCTEETTLYYVVSEDFNGI
                     GLDELILGNYLHLEAIVNIMIQVLKALEMIHRNHSYHGSLKSSSIIVDTEYNIKICDF
                     GITKANNGVNTRSYGNRRYLCPHQLCINYTDKESDFFATGLILFESIFKKLPFGESNS
                     EEKMLQSIDKGLDWNSIKAINGNTELINVIKRLLGRNNKYTRANDIIIDLSKVMYEKA
                     YIEVEDEKEEEQKQDKVIQVKKEYKSKMRKKLHKKLVVAGLAIVMISMIIISSII"
     gene            5633..6700
                     /locus_tag="PC471_003036"
     CDS             5633..6700
                     /locus_tag="PC471_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
                     /protein_id="HDF2790144.1"
                     /translation="MSNIKIIASNKCWIEDIARKQLEDISKLNGILRVVGLPDLHAGK
                     IPVGLAVETKNIIYPHIIGNDIGCGMTLFKTGVLKKKFKKDKWIKSLSKIKDLSDIEI
                     KNTYKEECPILNLGTIGSGNHFAEIQCISEIYNKEQFEQLKFSSDDIMMLVHCGSRNY
                     GEDILKKFYDKDGLEVESEKAIDYIKKHDNALMWAERNREAISKKIMQSIGTSGDVET
                     IFSINHNFIEKREDKFIHRKGAVSSERGAVIIPGSRGSLSYIVMPTENTEISLYSLSH
                     GAGRKWSRSVCKSRLKSKYSKDTIKQTKFKSQIICNDVNLLFQEAPEAYKNIEQIIES
                     LIEYELIQVVATMKPLITYKG"
     gene            7234..9144
                     /locus_tag="PC471_003037"
     CDS             7234..9144
                     /locus_tag="PC471_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HDF2790145.1"
                     /translation="MNSNTFERLQLNEVKDLIKIHCVSSLGKNLIDKLTPSGNIGVVR
                     RKLAENKEARKIIENSNHIPLEGLFNVGSTIDKIEKGMIIEPVELVNIEDFLRGCRKM
                     KAFMLEKEFYSPTLSSYALNITECKSIEDEINYCIKSNKVDSNASKELKKIRRNIEIT
                     EGKIKDRLNKFITSTVNKKYIQEFIISKRNDRYVIPIKSSYKNEVNGTILDTSSKGNT
                     VFIEPISVSNLSTELTMLKADETIEEYKILSYLTELIFEKISQIKLNIEILSEYDMVF
                     AKAKYSQKIKGITPKINNNGYIKIIKGKHPLLTGDAVPLDFEIGKNYRSLIITGPNAG
                     GKTVTLKTVGLLTLMVQCGFDISAKEGSEFSVFEKVFVDIGDNQSIENALSTFSSHIK
                     NIAEIMSLSNNSTLVLFDEIGSGTEPNEGAGLAISLLEEFYKMGCITIASTHYGEIKK
                     FATLHPEFENAGMMFDKETLEPLYKLTIGKSEDSNALFISKKMGIKNRVLDRAKEYVI
                     DRNYNLDLVKRSKLQSNIEDKNSSEVILNYTDYNVGDKVKLLDEDDFGIVYKPMDKFN
                     NVEVLFNDNFISVNIKRLQLSIKASELYPEGYDLDVLFTSFKERKLEKDIQRGSKKAL
                     KKIQKEIRENRK"
     gene            complement(9207..9830)
                     /gene="ytaF"
                     /locus_tag="PC471_003038"
     CDS             complement(9207..9830)
                     /gene="ytaF"
                     /locus_tag="PC471_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation membrane protein YtaF"
                     /protein_id="HDF2790146.1"
                     /translation="MIESILLVTSLCIDAFVTSFGYGVNKVKVPLYSNIIISVVCSAI
                     LAISLFAGTIVRDFLPQNITMLICFFILFLLGSARLIESFFKSYFKKKSIHSKNYNFN
                     MLDLKLNFNVSIEPDVLDTSKSKVLKSAEAFTLALALSLDGLAIGFGSGLVGVNYIQV
                     ILFSLISNIIAVLLGCLLGNMFVKNVNLNLSWLSGLILLVIGFMKIC"
     gene            10018..10662
                     /locus_tag="PC471_003039"
     CDS             10018..10662
                     /locus_tag="PC471_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF2790147.1"
                     /translation="MEQNNKVGNFGDLLKILLDEKELSMGELSKKSGIDKSTISRIAN
                     NKQKPNINHLEKMAIHLNINLEELLKASGYEFRNSNNQIFNADSDFSNFDDILGFANL
                     INDKNFNGNIEKELSKCKLYVQTDEGKKLLFDNFNKKIDSIEKQGQFTDRLRQMYADF
                     CTDGLTIKKYLLIGSMLLYFVISTDVIPDFVFPIGFMDDLVALNIVTKLLKNDK"
BASE COUNT         4364 a         1053 c         1753 g         3498 t
ORIGIN      
        1 attaatatat gtcaatagtt aggtatatat gtatttaatt agcatgatat agataaaagt
       61 atttatttta attgtctatt atctgaaaaa gtctttactt agaaatcata aaaatcaaat
      121 taattaggaa aagaaagggt gtgtataatg tgattgagac tggatttgta tattgtagtt
      181 tcttggttgt attcgtaggt gtaatttttt ttatggttga taaattgaaa tggaaggtat
      241 ttgatgttat taatccaatg cttttaatat acttaggttc agctatactt ggatcatttg
      301 gtttatttgc acagaatgaa gatgttggaa cgtatcaaaa cataataaca gtgaacttcc
      361 ttccattaat gttagtgttt ttgttaattc aatgtgattt aagaaaaata ttaaagatgg
      421 ggcctaaaat gttattatca tttttctgtg ctacatttac atttatgctt ggatttgtta
      481 tagcattcat aatttttaaa aattcaatgg gtgcagattc atggaaaatt cttggggcag
      541 catctggttc atggataggt ggctcatcaa atatgattgc agcagctaat gctctaggag
      601 ttgcagaaga aggacttagt tatgcaatac ttatgggttc tataggatat acagtatgga
      661 tgtctgtttg tctaatgtct gtgaaatttg gacctaaatt taacaaatgg acaaagtgcg
      721 atacaagttt tatagatgag gttacatcaa aacttgaaaa agaaaataaa gataatacag
      781 cacctacgtt tgtagaactt atgattttac tttctgttgg gtttggagtt gcgtcttttg
      841 cacaaattgt atccaagata cttccaactt caggtatact taatgaaact atgtgggtag
      901 tacttgttgc atctacagta tcaatattat tgggtatgac aaaaatatca aagcttaaag
      961 ggagtacact ggtaggaaat gtgtttatgt atttactcct tgcagctaca ggtagtaaag
     1021 caaatttctt tggattgtca gaagcacctg tttacataat gtttggattg gtagtagtcc
     1081 ttgtacatgc tatttcattt atagggcttg ctaaactatt taaacttgat ttatttactt
     1141 gtgaagtagg aagtatagct aatataggag gagtaactac agctccaatg attgctgggg
     1201 ttcacaatcc tgtattaata ccagtaggtg tacttatggg gcttctagga aatattatag
     1261 ggacttattg tgctcttatg gtaactcaaa tattacatat gttagcttaa taaaaatatg
     1321 gtataaaatt aataatttaa ataagaaaag tcatttcaat ttaagcagaa gcttttactg
     1381 aaatggcttt tttgtttgac aagataatct atttcaagaa gatgttttga catatttagt
     1441 gtataatatt gtaaaattta ctaaaaacga gatatattta atttttatat tgaatcgaac
     1501 gtctgtatga tgtataataa atatacagaa cgaatgtttg cagaacgggt gttttttgaa
     1561 tttattttaa taatttatga atatatatca gaatgcataa atatatttaa atataaagat
     1621 taaaaagaat ttaaaggagt gttgataact tatgataatt ccatcaagaa gaatacaaga
     1681 tataacatta acaaatttaa aaaatggtga agtaacttta attgaattgg atgaaatata
     1741 caagaagatg ggatttttat ttgttgttag tgaaggcaaa ttgaaaaaga taaaaaagga
     1801 gaatcatcat tagaaatgaa tgaaataata aatattataa tagtttataa aacctaactt
     1861 atgtagaata taaatactta agttaggttt ttttgtgata aagtttataa gtactcaatt
     1921 ttataagata aataatggtc ttaagtgaaa agtaaataag tttaaatttt agtaagtatt
     1981 atattgcata agaggtgata ttattatgaa aatagtaacc tacaatattc ataaaggtat
     2041 ggattcaaat aatagattaa ctctaactaa aatggggtta tatttaaaac aattggattg
     2101 tgatgtaatt tgtttgcaag aagtattata tcctcaattt ttagctttaa aagctgtttt
     2161 aaatatggat ggtgtctttg cgacaaatgt aaaaaaagta aatatgatat atggtatttg
     2221 tacatttaca acatctaaaa tgttaaacag taatcatttt ttcttaacta gcaaaaaaga
     2281 acaacgggga gctctttgta taactataga tgcatatgga agaattataa atgtaataaa
     2341 tactcacttg ggacttgata ggcaggagag agcaaaacaa ttagacgaaa taatagacta
     2401 taggaacaga ttagtgggga tagttatttt atgtggtgat tttaatgaga aaaatgtctt
     2461 tttaagtatg tttaatgata tggctatttc actaaacaag tcttacttac caacatttga
     2521 aaaatcaaac tctagaatag attacatttt tgttaataaa aatactgaac taaaaggata
     2581 cacagtagaa aaaatttacc tgtcagacca ttatcctgta ataggttaca tttaaatttc
     2641 aatataaatg aaattagttg ttataaaata aacaattttt tgttaagaaa tagtaaaaga
     2701 aatataataa aaaatatcaa aaataaaact aattataaga cataaattta caataaagtt
     2761 acatttgatt aaattccttg tttaacaaaa gctattcaaa tataatctaa ggtagttttg
     2821 aaatgggaac tctattatgc aaatttaagc tagtgttaaa atgaggtgaa gttatgggca
     2881 gaagaacaac aagagtttca agattaggta aagaaaaaaa acgaagtgtt ttaattgtat
     2941 tggcagcttt agttctaata atgggtggat ttacttattt ttttaacagt aaatttctgt
     3001 acaatgggaa aatcgctaaa aatgtatata tagaaggagt aaatgtatct gatatgacta
     3061 aagcagaggc attaaaagct ataacagata agtatactcc agaagattta aatcttgctt
     3121 atgatgggaa aaaatataca atatcaccaa aagatattga tttaaaatat gatacagaag
     3181 atgttgtaaa agatgcttat gaaagtacaa aaaaagggtc ctattttcaa aacttaaaaa
     3241 aatatattga tataagagtg aataaggcaa atatgaagat aaaagctgaa tatgatgagg
     3301 caaaacttag ttccaaagtg tcttctatag cagatagtat aaatgttaag atgaaaaatg
     3361 cttctattag tgttggaagt ggtggactaa gttatacaga ctctgttgta ggtagagaat
     3421 ttgatttggc agcaaataaa gagtctatat ataatatgat aaaaaataaa gagcataaaa
     3481 ctttagaact taaagtaaat cttcaaaaac cagatataac aactgaacaa gtaaaaactg
     3541 taaattctgt tataggtcaa tattcaacta cttattcaca agctgtagag ggtaggtcat
     3601 ataatgtagg attatcagca agaaaaacta gtgatgtatt attaatgcca ggtgaagaat
     3661 tttcatacaa taaacttaca ggacctagta ataaagcaaa tggttacaaa gatgcacctg
     3721 ttatagttta tggtaagcta gaacaatcag caggaggagg tgtttgtcaa acatcttcaa
     3781 cagtttataa tgcggctctt ctttcgggta tggagataac tcaagttaca aatcattcat
     3841 ctgcatctac ttatgtgcct aaagggagag atgctactgt gagcgatgga ggattaaact
     3901 taaagtttaa aaatccatat aagcatcctg tatatattaa aaattatgca ggtggaggaa
     3961 gtgtatcatc agtaatctat ggtaattctg gtgacaaacc aaatatatct atagaggtaa
     4021 aacaaactgg tcagaataaa tattctacat atcgaatatt taaagattca agtggaaagg
     4081 tcataaaaaa agaacatata tcaaatagtt cttataaaga attaaaaaaa tagataattt
     4141 tttagagaaa tatttgtaat ataaaaggga gtgtctcaaa atgaactttt tagtttatga
     4201 gacactcttt ttctgtgaaa tcaaatatat tttcatcatt tcaacaatac aaaagaagcg
     4261 tatctctatt ttgagacagc cttttttgta aaaattaaca ctattagttc ttgttttaat
     4321 attacaatca ttaatatata gaaaatattc ctatatattg gtataattgt aaatagattt
     4381 agttatataa aatgtatgag gaggtgacat ggttgaattt tataggcaat agatatgagg
     4441 tagtaaattc tgacgacgtt ttagaaatta acaaaatcta taaagctaga gatgtttttt
     4501 acaggaaaaa tgtgttaata aaagttataa agcacaataa ttatatctgt gaagattttg
     4561 tttcaaattt aattgatgaa agtactgctt tagatgagat aaactctcct tatatactaa
     4621 aaataataga tgttgggatt cactgtacag aagaaacaac attatattat gttgtaagtg
     4681 aagactttaa tggtatcgga ctagatgaat taattctagg taattatctt catttagaag
     4741 caattgtaaa cataatgata caagttttaa aagctttaga aatgatacat aggaatcatt
     4801 catatcatgg tagtttaaaa tcgagtagta taatagtaga tacagaatac aatataaaaa
     4861 tttgtgattt tggaattaca aaagcaaata atggggttaa tacaagaagc tatggaaaca
     4921 ggagatattt atgcccacat caactatgta taaattatac tgataaagaa agtgattttt
     4981 ttgcaacagg tcttattcta tttgaatcta tatttaaaaa attgcctttt ggagaatcaa
     5041 attcagaaga aaaaatgcta caatctattg ataagggatt agattggaat agtataaaag
     5101 caattaatgg aaatactgaa cttataaatg ttataaaaag gcttcttgga agaaataata
     5161 agtatactag agccaatgat attattattg atttaagtaa agtaatgtat gaaaaagcat
     5221 atatagaagt tgaagatgag aaagaagaag agcagaaaca agacaaagtt atacaagtta
     5281 aaaaagaata taaaagtaaa atgagaaaaa aattacataa aaaacttgtt gttgccggtc
     5341 tagctatagt catgatttca atgattatta ttagttccat aatataaaaa ttgtatatta
     5401 agtattttaa aatcaatgtc tataaaggat atacatagaa attttagtgt agtcttaaat
     5461 tatgaaagga agtggttatt atgttaacaa aaaataatga ggtattaaac gtctaggagt
     5521 attttaacaa atagccacct gcttttacag tttagaataa aaaagctgtg gctaaaggtc
     5581 acagcttttt gctacttttt aaaggcagga gaatattaaa aaggagcaaa ttatgagcaa
     5641 tataaaaata atagcaagta ataaatgttg gattgaagat atagcaagga aacaacttga
     5701 agatatttca aagttaaatg gaattttaag agtagtagga ttacctgact tacatgcagg
     5761 taaaatacct gtaggtttag cagttgagac taaaaatatt atttatccac atataattgg
     5821 aaatgatata ggatgcggaa tgactttatt taaaactggg gttctaaaaa agaaatttaa
     5881 aaaagacaaa tggattaagt cacttagtaa gattaaagat ttgtctgata tagaaataaa
     5941 aaacacatat aaagaagaat gtccaatttt aaatttagga actataggga gtggaaatca
     6001 ttttgcagaa attcaatgta taagtgaaat ttataataaa gaacaatttg aacaattaaa
     6061 attttcatct gatgatatta tgatgttagt tcactgtggg tcaagaaatt atggagaaga
     6121 tattttaaaa aaattttatg ataaagatgg acttgaagtt gaaagtgaga aagctataga
     6181 ttatattaaa aaacatgata atgctttaat gtgggcagag cgtaatcgtg aagctatcag
     6241 taaaaaaatt atgcagtcaa taggtacatc tggagatgtt gaaacaatat tctctataaa
     6301 tcataatttt atagagaaaa gggaagataa atttatacat agaaaaggtg ctgtttcaag
     6361 tgaaagagga gctgtgatta taccaggttc aagaggcagt ctatcatata tagttatgcc
     6421 tactgaaaat acagagatat ctttatattc tctatcacat ggagcaggta gaaaatggtc
     6481 tcgttcagtt tgtaaatctc gccttaaaag taagtatagt aaagatacta taaaacaaac
     6541 taaatttaaa agtcagatta tatgtaatga tgtaaattta ttatttcaag aagctccaga
     6601 ggcatataag aatatagagc agattattga aagtttgata gaatatgaac ttattcaagt
     6661 agtggcaact atgaagccat tgattactta taaaggataa tttttaaagt taaaaaagat
     6721 taaaattata ttataaatag ctaattttat ggaacattta aaagtgtttt attattagct
     6781 atttttttat tttgtagaga cgataattta tagagctaaa ttctttgata tataatgatt
     6841 acaaagttag actgtattgg aaactgcaca tacatgaatt gatgacaata taagtgattc
     6901 ccaaatataa atgaaagtat aagcttattt ttactataaa aattttcact gtacaaaaac
     6961 attaatgaca ttatttttat tgacataaat atataattta tggaataata ttagaagcac
     7021 tgatataagt gtttaaataa gaatagaaaa ggagaagtgt tttatgaaaa aaaatactaa
     7081 cattaatgag tccggagtta aacttcttaa taaataagga gtttgatata taataactcc
     7141 ttatttatta agttgttcgc aaaataacta ttatcaaagt aaatgatagg gtgatttaaa
     7201 agacgatgac ttataaataa aggagaaaaa ataatgaata gtaatacatt tgaaagatta
     7261 caactaaatg aagttaaaga cttaataaaa atacattgtg taagttcgct aggaaaaaat
     7321 ttaatagata aattaactcc tagtgggaat attggagtag taagaaggaa attagcagaa
     7381 aataaagaag ctagaaagat aattgaaaat agtaatcaca taccacttga aggattgttt
     7441 aatgtaggtt ctacaataga taaaattgaa aaaggaatga taatagaacc tgttgaactt
     7501 gtaaatattg aagatttttt aagaggttgt aggaagatga aagcttttat gttagaaaaa
     7561 gaattttatt cacctacatt aagttcttat gccttaaata taacagaatg taaaagtata
     7621 gaagatgaga taaattactg tataaaatct aataaagttg attctaatgc tagtaaagaa
     7681 ttaaaaaaga taagacgaaa tatagaaatt acagaaggaa agataaaaga tagattaaat
     7741 aaatttataa catcaactgt aaataaaaag tatatacaag agtttataat aagtaaaaga
     7801 aatgatagat atgtaatacc aattaaatct tcttataaaa atgaagttaa tggtactata
     7861 ttagatacat catcaaaagg aaatactgta tttattgaac ctattagtgt ttcaaattta
     7921 agtacagagt taactatgct aaaagctgat gaaactattg aagaatacaa aatattatct
     7981 tatttaacag aacttatatt tgagaagata agtcagataa aattaaatat agagatactt
     8041 tcagaatacg atatggtatt tgcaaaagct aagtatagtc aaaaaataaa aggaattact
     8101 ccaaaaataa ataataatgg ctatataaaa ataattaaag gtaaacatcc acttctaaca
     8161 ggagatgcag tacctttaga ttttgaaata ggcaaaaatt atagaagtct tataataaca
     8221 ggtcctaatg caggaggaaa aactgtaacc cttaagactg ttggtttact aactttaatg
     8281 gttcaatgtg gatttgatat ttctgcaaaa gaaggtagtg aatttagtgt atttgaaaaa
     8341 gtttttgtag atatagggga taatcaaagt attgaaaatg ccttaagtac tttttcatca
     8401 catataaaaa atattgcaga gattatgagt ttatcaaata attctactct tgttctgttt
     8461 gatgaaatag gaagtggaac tgaacctaat gaaggagctg gtttagcaat atcattatta
     8521 gaagagtttt acaaaatggg atgtataact atagcatcaa ctcattatgg agagataaaa
     8581 aagtttgcta cattgcatcc agaatttgaa aatgctggta tgatgtttga taaagaaacg
     8641 ttagaaccat tgtataagtt aactattgga aaatctgaag atagtaatgc tctttttatt
     8701 tctaaaaaaa tgggaataaa aaatagagtt ttagatagag ctaaagaata tgtaattgat
     8761 agaaactaca atttagattt agttaaaaga agtaagctac aatctaatat tgaagataaa
     8821 aatagttctg aagtcatttt aaactataca gattataatg taggggataa ggtaaaatta
     8881 ctagatgaag atgattttgg aattgtatat aaacctatgg acaagtttaa taatgtagaa
     8941 gtattgttta atgataattt tatttcagta aatataaaaa gattacaact tagcataaaa
     9001 gcatcagaat tgtatccaga agggtatgat ttagatgtat tatttactag ttttaaagaa
     9061 agaaaattag aaaaagatat tcaaagaggt tcaaaaaaag ctcttaaaaa gattcagaaa
     9121 gaaattagag agaatagaaa ataaatataa aaagcacaaa agaaagcaat ctaaagagaa
     9181 aagtttatct ttagattgct ttcttattaa catatcttca taaatccaat gactaacaag
     9241 attaaaccac ttaaccaaga taagtttaaa tttacgtttt taacaaacat atttcctagt
     9301 aaacaaccaa gtaatactgc aattatattt gatattaaag aaaatagaat aacttgtata
     9361 taattcacac ctacaaggcc actaccaaaa ccgatagcta atccatctaa acttaaagca
     9421 agtgcaagtg taaatgcttc agcagattta agcactttag atttacttgt atctaagaca
     9481 tcaggttcga tacttacatt aaaatttaat ttaaggtcta acatattaaa attataattc
     9541 tttgagtgta tagatttttt cttaaaataa gatttaaaaa aactttcaat tagtctagca
     9601 cttcctaata aaaataaaat aaagaaacat attaacattg ttatattttg aggtaaaaag
     9661 tctctaacta ttgtacctgc aaaaagcgat attgctaaaa tagctgaaca aactacgctg
     9721 ataattatat ttgaatataa gggaacttta actttattta cgccatatcc aaagcttgtc
     9781 acaaaggcat ctatacaaag agaagtaact aataaaattg attcaatcac agtagattaa
     9841 catcctttct cttaaatttt gtacttatat tagttatagt attgtaaaaa tggataaatt
     9901 gttacagata atccaataaa ttgttgataa aattccaaca tcatacaaca cctaaataac
     9961 aaaagaatca tatcatataa taatataaaa ccaatcataa taaagcgggt gaaaataatg
    10021 gaacaaaata ataaggttgg gaatttcggt gatttgttga agattttatt agatgaaaaa
    10081 gaattatcaa tgggagaact aagtaaaaaa agtggaattg ataaatctac tatatctcgt
    10141 atagctaata ataaacaaaa gccaaatata aatcatttag aaaagatggc aatacattta
    10201 aatattaacc tagaagaact gttgaaagct tcaggatatg aatttagaaa cagcaataat
    10261 caaatattta atgcagatag tgattttagt aactttgatg atattcttgg atttgcaaat
    10321 ttaatcaatg ataagaactt taatggaaat attgaaaaag agttaagtaa gtgtaagtta
    10381 tatgtccaaa cagatgaagg taaaaaatta ctttttgata attttaataa aaaaatagat
    10441 tctattgaaa aacaaggtca atttacagat agattaagac aaatgtatgc tgatttttgt
    10501 acagatggac ttacaataaa gaaatattta ttgattggaa gtatgctact ttattttgtt
    10561 atatcaacag atgtgattcc agacttcgta ttcccaatag gatttatgga tgatttagta
    10621 gcattaaata tagttacaaa attacttaaa aatgataagt aagtttta
//
LOCUS       DAMJYR010000122        10538 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.127, whole genome shotgun sequence.
ACCESSION   DAMJYR010000122 DAMJYR010000000
VERSION     DAMJYR010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10538)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10538)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10538
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.127"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            23..1723
                     /locus_tag="PC471_003040"
     CDS             23..1723
                     /locus_tag="PC471_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2790148.1"
                     /translation="MKRYLKTYGVALIIVIIYAFIKLPVLRLDFTSLFSVGIIFFVVA
                     GILDMMLDRNERASKMAKYNFSIAIALLVYIVVVPFITSTPVLHAKSYRELLGKVTES
                     KFTDDISPVSVNDIRLVDEDMAMKLGDKKLGEVPAIGSVSKLGKFHIQNVDGELYWVA
                     PLVHRDIIKWITSLSGTSGYVKVSASNPQDVQLVQEIDGKPIKIVYQPEAYLHQDLQR
                     HLYIHGIVNVGMTDFTLEINDEGRPYWVVSLYEHKIGYGGANATGIATVDAETGKINV
                     YDVKNTPKWVDRIQPQSFVTDQIKDWGVYVNGFLNSVISEKGVLVPTEGTSLVYGNDN
                     RSYWYTGITSSGGDESTIGFMLVDSRTKEARLYKQPGATETAAMKSAEGNVQEKNYEA
                     TFPVMYNILGQPTYVSSLKDKAGLVKRVAFVSVEDYNVLGIGEDKNEALRNYKDALES
                     KGNDLKLDNDLKDEVLEGTVTRISPDVRGGNTNYYVTLDSNKDIIFRATSKVSSELPL
                     TQVGDKVKISYSKEESGVIEMSEFDNLNIANFEDKKAKEKTGTDENTDSKDSENQGNV
                     "
     gene            1893..2510
                     /locus_tag="PC471_003041"
     CDS             1893..2510
                     /locus_tag="PC471_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2790149.1"
                     /translation="MTNMEKEILLLISRVRCVTESQFRKIYGDQKRYNKKNFKKTLRK
                     MCSEYTLKKYPCDVDYYNYKDLSYVYYLNGSKLFKGDDLIKALVGSELVVRINTAGCD
                     VKRFYRNVSVDDVKYDLFIEYTDQFGNIKQVLVDVDLNNEIDISKYENLSKKIDKSTI
                     PFFKVPNVLVVTPDNLSELNDLERYDNEYSINFIDLGLNKLFNCI"
     gene            2871..4064
                     /locus_tag="PC471_003042"
     CDS             2871..4064
                     /locus_tag="PC471_003042"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HDF2790150.1"
                     /translation="MSIIVQKYGGSSVADTEKIKSIAENIIERRKENPQMVIVVSAMG
                     KSTDEYITLAKELSNEPSKRELDALMSTGEMISASLLSIALNALGCKAISYNAYQLNI
                     KTSGLHGKSQIDDINVNRIKNSLDEGNVVIVTGFQGINEDGDVTTLGRGGSDTSAVAL
                     AVKLNGKCEIYTDVDGIYFTDPRKYSKASKLDEIEYEEMLELASLGAQVMHSRSIELA
                     QKYGVEIYVGRTCGTEKGTYIRGGKDMKLEDKVITGLATSDDDSSITIKDFKAENISS
                     LFEDIATIGISVDMISQTAPILDKISVSFTVPKEELGECKKIVSKYTDEEHLVIDNNI
                     TKFSLVGLGMKNTSGVAAKVFKIFNENGIMIKLITTSEIRITCAINSDDKQVAIEKIA
                     EVFNI"
     gene            4082..5374
                     /gene="lysA"
                     /locus_tag="PC471_003043"
     CDS             4082..5374
                     /gene="lysA"
                     /locus_tag="PC471_003043"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HDF2790151.1"
                     /translation="MKLHGTMTVNNDELYIGGVSCLELSKKYQTPLYVFDEALVRQNC
                     REYIEYFKAREGKNKVAYAGKAFLPLYMCNLINEEQMCLDVVSGGELYTAYKSNFPME
                     KVFFHGNNKTKEEINMGLDLGVGKFVVDNFYELDLLEALCEDKGKTQEIYFRITPGIE
                     AHTHDYIKTGQIDSKFGFALVNGDLFRAIEKLSEYKNIKLVGLHAHIGSQIFDVEPYI
                     DTVDIMMNLVKEIKEKFNIQLTEIDLGGGVGVYYSEGDKPKTIKEFCETIITKAETSC
                     KELGIEVPTLVIEPGRSIVANAGSTLYTVGSIKDIKDVRVYVSVDGGMTDNVRPSLYQ
                     ASYECSIVNKINHEAMNHVTIAGKCCESGDILIGDINIMDIKSGDILITTSTGAYGYS
                     MSSNYNKIPRAAVVSVFDGNDKIICKRQSYEDLLALEI"
     gene            5591..6250
                     /locus_tag="PC471_003044"
     CDS             5591..6250
                     /locus_tag="PC471_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HDF2790152.1"
                     /translation="MRGRVILLISILSIFLVGCSFNKKDEINLVEQGKTYLEKHNYKK
                     AMESLSSALEEDSTNENARAMYMQAMRMSNMTEFEELKNYEGAIKELKLIEKIKNGSS
                     VIKEEATKKKEELTKLEKEQNEAEEQRKKKAKDTAGEDRYRVESDARKSSYSGSSKNN
                     KSSSKNNDKKSDSNKSDNNNSSSQNQEKPNNSNPTPAPTPQDTTGGSDSGGNSQNSNN
                     Q"
     gene            complement(6405..7490)
                     /locus_tag="PC471_003045"
     CDS             complement(6405..7490)
                     /locus_tag="PC471_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HDF2790153.1"
                     /translation="MIIISKENIKYYLIVVFIGILFFKFINTPSDFISSIEGFLKFFS
                     PFLLAILLALLLNPLVMLFEIKFKAHRLLAIFLSYIFIGFILAFAIRLLIPSIANTLN
                     RLINEMPMYTDYIDSFIEKNMSNIYFLKTLIPHIQHSLDNLLKEASNFVGKIPKNFLI
                     YTLSISSMLFNMTMGFILSIYIIYDKEKIALGFKRFLYSSTARNKADNIIEFFRTTHD
                     IFYDYLLGRILDSLIIGIIAFLGFQFVIRIENALFLASIIFLGNIIPYFGPFIGAIPP
                     IAMTILYSPQKTIWVIAFLFILQQLDGNFIEPKVMGNQVGIGAIWVISAILVGQSLFG
                     FIGVFLSVPIAAVVKTYVDKYIDNRLQ"
     gene            7681..8397
                     /locus_tag="PC471_003046"
     CDS             7681..8397
                     /locus_tag="PC471_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HDF2790154.1"
                     /translation="MRINKYIASCGIASRRKAEEIILGGRIKVNGSVVKELSFNIDEE
                     KDIVEFDNKKVKPSENYIYIVLNKPEGYITTVKDQFNRPSVIDILKDVRERVYPIGRL
                     DYETSGLLILTNDGDLTYKLTHPKHEIDKMYVASVKGIISDDEIRQFETGLKIEDYTT
                     APAKIRVTKENKEKNYSVCEITIHEGRNRQVRKMCKAINHPVLNLRRISVGKIVLKDT
                     KVGEYRYLTEDEIKYLKSIK"
     gene            8455..9033
                     /locus_tag="PC471_003047"
     CDS             8455..9033
                     /locus_tag="PC471_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HDF2790155.1"
                     /translation="MAYAKYLTKITDINKFYLENIINEGDIVIDATMGNGYDTRYLAE
                     KVGEKGFVYAFDIQEEAIKSTRKKLEKEGYTDRVKLIFDGHEKMNQYIKEEVSCVLFN
                     LGYLPRAKHSIITKPDTTLKAIKLSLEMLKENGVISIAIYTGHDGGQEEKDCIYNLVN
                     NLNQDEFNVLESKFLNQVNNPPQLILIEKKKA"
     gene            9427..10299
                     /locus_tag="PC471_003048"
     CDS             9427..10299
                     /locus_tag="PC471_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDF2790156.1"
                     /translation="MEETNEMKDSKHLISNIQSQYTRLSKGQKLIAQYILNNYDKVAF
                     MTACKLGETVGVSESTVVRFANALGYSGYPKLQAALQELIKNKLTTVQRVEMAHDYSD
                     DFAILNKVLKSDIDNIRSTLEEIDERAFKEASNKLLRARKIYILGMRSSFVVAQYLGF
                     YLDIILDNVHIIRMDMGDAFEQIVRINEEDVIVAISFPRYSKKSYQIVNYAKEKGAHV
                     ISLTDSLFAPVASLADNTLLVKSNMASFVDSLVPALSISNALAISVGMKEKEDIKQHF
                     DDLEQIWKRYSVYE"
BASE COUNT         4349 a         1111 c         1838 g         3240 t
ORIGIN      
        1 attttttaga gaggagtatt aaatgaaacg atatcttaaa acatatggag ttgcattaat
       61 aattgtaata atttatgcat ttattaaatt acctgtacta agattagatt ttacatcttt
      121 attttcagta ggaataattt tctttgttgt agcaggaata ctagatatga tgttagatag
      181 aaatgaaaga gcatctaaaa tggctaaata taatttttca atagcaatag ctttgcttgt
      241 gtatattgta gtagttccct ttataacttc tacacctgta cttcatgcaa aatcatatag
      301 agaactattg ggaaaagtaa ctgaaagtaa atttactgat gacattagcc ctgtcagtgt
      361 aaatgacatt cgtcttgtag atgaagatat ggcaatgaaa ttaggtgaca aaaagcttgg
      421 agaagttcct gcaataggga gtgtatccaa attaggaaaa tttcatatac aaaatgtaga
      481 tggagagttg tactgggtag cgcctcttgt tcacagagac ataattaaat ggataacatc
      541 tctaagtggt acaagtggtt atgtaaaagt atcagctagt aatcctcaag acgtccaatt
      601 agttcaagaa atagatggaa agccaataaa gatagtttac caaccagaag cctacttaca
      661 tcaggattta caaagacatt tatacataca tggtattgta aatgttggta tgacagattt
      721 cactcttgaa ataaatgatg aaggaagacc ttattgggtt gtaagtttat atgaacataa
      781 aattggctat ggaggagcaa atgcaacagg aatagccact gttgatgcag aaactggaaa
      841 aataaatgtt tatgatgtta aaaacacgcc taaatgggtg gatagaatac aaccacaaag
      901 ctttgtaaca gaccaaatta aagactgggg tgtttatgtt aatggatttt taaactctgt
      961 tatatcagaa aagggagttt tagtaccaac agaaggtaca tcattagttt atggcaatga
     1021 taacaggtct tattggtata caggtataac atcttcaggt ggagatgaat ctacaattgg
     1081 atttatgtta gttgattcta gaacaaaaga agctagatta tataaacaac caggagctac
     1141 tgaaacggct gcaatgaaat cagctgaagg taatgttcaa gaaaaaaatt atgaagcgac
     1201 gttccctgta atgtataata tattgggtca gccaacatat gtatcatcat taaaagataa
     1261 ggcgggtctt gttaagagag ttgcatttgt atctgttgaa gattataatg tacttggaat
     1321 aggtgaagat aagaatgaag cattaagaaa ctataaggat gcattagaat ctaagggtaa
     1381 tgatttaaaa cttgataatg atttaaaaga tgaggtatta gaaggcacag tgacaagaat
     1441 aagcccagat gtgcgaggtg gtaacactaa ttattatgta actttagatt caaataaaga
     1501 tataatattt agggctactt caaaagtatc tagcgaattg ccattgactc aggttggtga
     1561 taaagtcaaa atatcgtatt caaaagaaga atctggtgta atagaaatgt cagaatttga
     1621 taacctaaat attgcaaact ttgaagataa aaaagcaaaa gaaaaaacag gaactgatga
     1681 aaatacagat tccaaagatt ctgaaaatca aggtaatgta taatttaaat aaaaagattg
     1741 ataatagtat ttaacatagt gatagctaat ttttatagaa tttaattcta taaaaattag
     1801 ctattttatt ttaattcttt gtaaatttaa tgtcataaaa ttaatataat attagtacaa
     1861 ctttgcaaat aatacttcaa ggagaatgta ctatgacgaa tatggaaaaa gagatattac
     1921 ttttaattag cagggttaga tgtgttacag agtctcaatt tagaaaaatt tatggagacc
     1981 aaaaaagata caataaaaaa aattttaaaa aaactttgag aaaaatgtgt agtgaatata
     2041 cactaaaaaa atatccttgt gatgtggatt attataatta taaagacctc tcttatgtat
     2101 actatttaaa cggtagtaaa ctttttaagg gagatgattt aataaaggct ctagtaggat
     2161 cagaattagt tgttagaatc aatactgcag gttgtgatgt aaaaagattt taccgaaatg
     2221 ttagtgtaga tgatgtgaaa tacgatttat ttattgaata tacagaccaa tttggtaata
     2281 taaagcaagt tttagttgat gttgacttaa ataatgaaat agacatttct aaatacgaaa
     2341 atctttctaa aaaaatagat aaatctacaa ttccattttt taaagtacca aacgttttgg
     2401 tagttacacc agataatcta tccgaattaa atgatttaga gagatatgat aatgagtata
     2461 gcataaattt tatagattta ggattaaata aactatttaa ttgtatataa gctaacttta
     2521 gaaaagaaaa ctatcgattt gccacatttt gtaaaataat tgttgcaata ttatatagaa
     2581 tatgataaaa ctatattaat aaattaatta aaaataatga gcctagggta gaggtagctg
     2641 tattcaatag tattcaatag gagctggtaa gcgttgataa ttgatgaaag ggtatatagc
     2701 cgaagaaatt ttgttgacgg acaaattttc tggacttatg cagaatatgc ataaggctgt
     2761 cacttttaaa gtggtgagct acaaggttac ttggtctatg aaatttacac gtccaaagta
     2821 tacctttggg cgtttttatt tatcaaaatt tagcttgagg gggagaaagc atgagtataa
     2881 ttgttcaaaa atatggtgga agttcagtag cagatactga aaaaataaaa tcaatagcag
     2941 aaaatataat agaacgaaga aaagaaaatc cacaaatggt aatagttgta tctgccatgg
     3001 gaaaatccac tgatgaatat ataactttag caaaggaatt gagtaatgaa cctagtaaga
     3061 gggaattaga tgctttaatg tctactggtg aaatgatttc agcatcttta ttatcaatag
     3121 cattaaatgc acttggatgt aaagctataa gttataatgc ttaccagtta aatataaaaa
     3181 cgagtggtct tcatggaaag tctcaaattg atgatataaa cgtaaacaga attaaaaata
     3241 gtttagatga aggaaatgtc gtgatagtaa ctggatttca gggaatcaac gaagatggtg
     3301 atgtaaccac tcttggaaga ggtggttcag atacatctgc tgtagctctt gcggttaaat
     3361 taaatggaaa atgtgagata tatacagatg ttgatggaat ttattttaca gacccaagaa
     3421 aatattcaaa ggctagtaaa cttgatgaga ttgaatatga agaaatgctt gagttagcga
     3481 gtttaggagc acaagtaatg cattcaagaa gtatagagct tgcacaaaaa tatggtgttg
     3541 aaatctatgt cggacgtaca tgtggtacag aaaaaggaac atatataaga gggggaaaag
     3601 acatgaaatt agaggataaa gtaataacag gattagctac tagtgatgat gacagttcta
     3661 taacaataaa agattttaaa gctgaaaata tatctagctt atttgaagat atagcaacta
     3721 taggtataag tgtagatatg ataagccaaa ctgcacctat tttagataag ataagtgtat
     3781 catttacagt tccaaaagaa gagttgggag aatgtaaaaa gatagtatct aaatatacag
     3841 atgaagaaca tttagtgatt gataataata tcactaaatt ttctttagta ggtcttggta
     3901 tgaaaaatac ttctggagtt gctgctaaag tatttaagat attcaatgaa aatggtataa
     3961 tgataaaact tataactaca tcagagattc gtataacttg tgctataaat tctgatgata
     4021 agcaagtagc tatagaaaaa atagcagaag tatttaatat ataaaatttg gggaggctat
     4081 tatgaaactt catggaacta tgactgtaaa taatgatgaa ttatatattg gaggagtgag
     4141 ttgtttagaa cttagtaaaa aatatcaaac tccactttat gtatttgatg aagctttagt
     4201 tagacaaaat tgtagagaat atatagaata ttttaaggct agagagggaa aaaataaggt
     4261 tgcttatgca gggaaagctt ttttacctct ttacatgtgt aatcttataa atgaagaaca
     4321 aatgtgttta gatgtagttt caggaggcga gttatataca gcttataaat caaattttcc
     4381 tatggaaaaa gttttctttc atggaaacaa taagactaaa gaagaaataa acatgggatt
     4441 agatttaggt gtaggtaaat ttgtagtgga taatttttat gaattagatt tgcttgaagc
     4501 attatgtgaa gataaaggta aaactcaaga gatatacttt agaataactc ctggtataga
     4561 agctcataca catgattata taaaaactgg acagatagat tctaaatttg gatttgcttt
     4621 agtaaatgga gatttattta gagcaataga aaaattaagt gaatataaaa atattaaact
     4681 tgtaggtctt cacgctcata taggttcaca aatatttgat gtagaaccat acatagatac
     4741 agtggatata atgatgaact tggtaaaaga aataaaagaa aaattcaaca tacaactaac
     4801 agaaattgac ctaggtggag gagttggcgt ttattatagc gaaggagata aacctaaaac
     4861 tataaaagag ttttgtgaaa ccataatcac taaagcagaa acttcatgta aagagcttgg
     4921 aatagaagta cctacattgg taatagaacc aggaaggtca atagttgcaa atgcaggaag
     4981 tacattgtat acagtaggtt ctataaaaga tataaaagat gttagggttt atgtaagtgt
     5041 agatggaggt atgacagata atgtaagacc ttcgttgtac caagctagtt atgagtgtag
     5101 tattgtgaat aaaattaatc atgaggctat gaatcatgta acaatagctg gaaaatgttg
     5161 tgagagtgga gatatattaa ttggagatat aaacataatg gatattaaaa gtggagatat
     5221 acttataaca acatcaactg gtgcatatgg atattcaatg tcttcaaatt ataataaaat
     5281 accaagagca gcagtggtat cagtatttga tggaaatgat aagataattt gtaagagaca
     5341 aagctatgag gacttattag ctttagagat ataaaataga gaagtatata ataaaaatta
     5401 aatattaaag tttattatga gcagatttgc taggatatgt atctctaaat taatttttgg
     5461 tttattgtat cgttcgtagc atatctgctt attttttgtt ttactaacaa atttcttgta
     5521 ataaatataa aatattttaa aaactttata attatgataa aatttaaagt atatgacaag
     5581 gagggattta atgagaggaa gagtaatttt attaatatcg atattatcta tatttcttgt
     5641 aggatgttca tttaataaaa aagatgaaat aaatttagta gagcaaggaa aaacatattt
     5701 agaaaaacat aactataaaa aagctatgga atcattatcc tctgcactgg aagaagatag
     5761 tactaatgag aatgcaagag ctatgtatat gcaagctatg agaatgtcaa atatgacgga
     5821 gtttgaagaa ttaaaaaatt atgagggtgc aatcaaggaa cttaaactta tagagaaaat
     5881 caaaaatggt tcctctgtta ttaaagaaga ggcaactaaa aagaaagaag agttaactaa
     5941 attagaaaaa gaacaaaacg aagcagagga acaaagaaag aaaaaggcaa aagatacggc
     6001 tggagaagat aggtatagag ttgaatcaga tgcaaggaaa tcttcttata gtggaagtag
     6061 taaaaataat aaatctagtt ctaaaaacaa tgataaaaaa tcagatagta ataagtctga
     6121 caataataac agttcttctc aaaatcaaga aaagccaaat aatagtaatc caacaccagc
     6181 accaactcca caagatacta ctggtggttc agatagtggt ggaaattctc aaaactcaaa
     6241 taatcaataa gtataaaata tatgcataaa tttaatgaac aaattatata ttataaaaaa
     6301 tatctcttag caattttttc ttactaagag atattttttg agaaataatt taataaatag
     6361 cagttaaatt ttaaactaaa aattaaagtc acagtagaga taatctattg taaacgatta
     6421 tctatatatt tatcaacata agtttttaca acagcagcta ttggtactga aagaaaaact
     6481 cctatgaaac caaatagaga ttgtcccacc aaaatagcag atattaccca tatagctcct
     6541 ataccaactt gattacccat aacttttggt tctataaaat ttccatctaa ttgttgcaat
     6601 ataaatagaa atgcaatgac ccatatagtt ttttgtggac tatataatat agtcatggct
     6661 attggaggga ttgcaccaat aaaaggacca aaatagggaa ttatattacc taagaatatt
     6721 attgaagcta aaaataaagc attttctatt ctgataacaa attgaaatcc taaaaatgca
     6781 attataccaa taataagaga gtctaatatt ctaccaagta aataatcata gaaaatatca
     6841 tgagtagtcc taaaaaattc tataatgtta tctgccttgt ttctagcagt agaagaatat
     6901 agaaatctct taaatcccaa tgcaattttt tctttgtcat atataatgta tatagatagt
     6961 ataaatccca ttgtcatatt aaatagcata gaagatatac taagtgtata gattaagaag
     7021 tttttaggaa ttttaccaac aaaattactt gcttctttta ataaattatc taaactatgt
     7081 tgtatatgag gaattaaagt ttttagaaaa tatatgtttg acatattttt ttctataaaa
     7141 ctatctatat aatcagtata cataggcatt tcatttatca atctatttaa tgtattagct
     7201 attgatggaa ttaataacct aatggcaaag gctaaaataa aacctataaa tatataagat
     7261 aaaaaaatgg ctaaaagtct atgtgctttg aattttatct caaaaagcat cacaagtgga
     7321 tttaaaagta aagcaagtaa aatagctaat aaaaatgggc taaaaaactt taagaatcct
     7381 tcaatactag atataaagtc tgatggagta ttaataaatt taaaaaataa aattccaata
     7441 aacacgacta ttaaataata ttttatattt tcctttgaaa taataatcac ttccttaaaa
     7501 tagtgtttag tcagctatta catattaata gtatgtgaaa agaaattaca attatttagg
     7561 aaaattaaat aatttttttg cttgttttta attaaggtct agtatagtat aataatgtag
     7621 caaaattaac attgataaaa atattaaaca cacataattt aagataagga aggattaagt
     7681 atgagaatta ataagtatat agcttcttgt ggaatagcat caagaagaaa agcagaagaa
     7741 ataattcttg gaggaagaat aaaggtaaat ggaagtgtag taaaagagtt atcttttaat
     7801 attgatgaag aaaaagacat agtagaattt gataataaaa aagtaaaacc aagtgaaaat
     7861 tatatatata tagtattaaa taaaccagag ggatatataa ctacagtaaa agaccaattc
     7921 aatagaccca gtgtaataga catacttaaa gatgtaaggg aaagagtata tccaataggg
     7981 agattagatt atgaaactag tggtttatta atacttacta atgatggcga tttaacatat
     8041 aagcttaccc atccaaagca tgaaattgat aaaatgtatg tggcaagtgt gaagggaatt
     8101 ataagtgatg atgagataag acaattcgaa acaggactaa aaatagaaga ttacacaacg
     8161 gcacctgcca aaataagggt aactaaagaa aataaagaaa aaaattattc agtgtgtgaa
     8221 ataactatac atgagggtag aaatagacaa gtaagaaaaa tgtgcaaagc tataaatcat
     8281 ccagttttaa atttaagaag gatttctgtg gggaaaatag tattaaaaga caccaaagtt
     8341 ggagaatata gatacctaac agaagatgaa ataaaatatt taaaatcaat aaaataagat
     8401 aaaaataaat tgagaaaaaa tgaaataaag ttaatagtag ggagaaatag tattatggca
     8461 tatgcaaagt atttaactaa aattacagat ataaataaat tttatttaga aaatattata
     8521 aatgaaggtg acatagtaat tgatgctact atgggaaatg ggtatgatac aaggtatcta
     8581 gctgagaagg ttggagaaaa gggctttgta tatgcttttg atatacaaga agaagcaata
     8641 aagtctacaa gaaagaaact agagaaagaa ggatatacag atagagtaaa attgatattt
     8701 gatgggcatg aaaaaatgaa tcaatatata aaagaagaag tttcatgtgt tttatttaat
     8761 ttgggttatc ttccaagagc gaaacattca attataacca aaccagatac aactttaaag
     8821 gctataaagt taagtttaga aatgttaaaa gaaaatggag taataagcat tgccatatat
     8881 acagggcatg atggaggtca agaagaaaaa gattgcatat ataaccttgt caataattta
     8941 aatcaagatg aatttaatgt tttagaatct aagtttttaa atcaagtcaa taacccacca
     9001 caattgattt taattgaaaa gaaaaaagca taataaatac taaaaaactt gacaagattg
     9061 tattaagagt taatatatat agtgtgacct attaaaaata ctttaaataa taagtataca
     9121 acagtttatt ctcaaaacaa aatatacatc taaatatgca aaataagata gataactttt
     9181 aaatgagtaa aaattgaaaa atttaaaaga aatattggat aaatattaaa aaatgcaagg
     9241 aaagatggta taataaattt aatcgaaaat actatgtgaa gtataaggta cagaaaagtt
     9301 agaattttat gagtttaaga agctatgata aaaactaaga tgaaattgag ataaaaaata
     9361 aaatttttag agaaaaagac agtgttaata aaacaaaata gtttatataa ctaaaaggag
     9421 aaaaaaatgg aagaaacaaa tgaaatgaag gacagtaaac atttgatatc aaatatacaa
     9481 tctcaatata cgagattgag taagggtcaa aaacttatag cacaatacat tttaaataat
     9541 tatgataaag ttgcatttat gactgcatgt aaattgggtg aaaccgtagg agtaagtgag
     9601 tctacagttg taagatttgc aaatgcatta ggttatagtg gatatcctaa attacaagct
     9661 gcacttcaag aactcataaa aaataaacta actacagtac aaagagttga aatggcacat
     9721 gattattccg atgattttgc aatactaaac aaagttttaa aaagtgatat agataatata
     9781 aggtctacac tagaagaaat tgacgagaga gcgtttaaag aagcttctaa caaactttta
     9841 agagcgagaa aaatatatat acttggaatg aggagttctt ttgtagtagc tcaatattta
     9901 ggtttttact tagatataat acttgacaat gttcatataa ttagaatgga tatgggagat
     9961 gcttttgagc agattgtaag aataaatgaa gaagatgtaa tagttgctat tagtttccct
    10021 cgttattcta agaaatctta tcaaattgtc aattatgcta aagaaaaagg agcccatgta
    10081 atatcattaa cagatagctt atttgcgcca gtagcatcac ttgcagataa cactcttcta
    10141 gttaagagta atatggcttc atttgtagat tctctagttc cagctcttag tatttcaaat
    10201 gcccttgcta tatctgttgg gatgaaggaa aaagaagata ttaaacagca ttttgatgat
    10261 ttagagcaaa tttggaaaag atattctgtg tatgagtaaa gtttgtatat taataaggct
    10321 atgaaattga gaatcatagc cttattagta aatactcgat tatcacttaa aaaataaatt
    10381 ttcgtttaaa taatacaaat aaatatttcg agactcaaaa cataaagatg tattctgaat
    10441 acatctttac tacgtcaact aatttattaa agaaagcatt tccattcatg gagagtgctt
    10501 tttttatttc tctaaacaat atcatttcct taattaac
//
LOCUS       DAMJYR010000123        10489 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.128, whole genome shotgun sequence.
ACCESSION   DAMJYR010000123 DAMJYR010000000
VERSION     DAMJYR010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10489)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10489)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10489
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.128"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            1..981
                     /locus_tag="PC471_003049"
     CDS             1..981
                     /locus_tag="PC471_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HDF2790157.1"
                     /translation="MNLKLIWINILNPVFIKNEIKRKKIQIIIFFFINFGLTYSLGLL
                     LYYNKFIDPENFASFMMILPLSSVAIAKFYTEGLNNDMYEFYSLIILFFLIYSLLIVL
                     KLLNLINNNEFIIINLILTFISSLSIIIYSSNKKNLYPVKNIKLGSLLIIYFMISVIV
                     PTSFILILQGAQPNYSGIIKFIFTPITSLTTIYIFFSEEYAWRGFLQNIFFDKFGKKL
                     GVIILGMCWSLWHLPLIFTLYTPEAPILGIILRSIHIVGISIFLGYLYIKTKNIWFCA
                     IIHVLNNSAFLITNSKESTITYHDILIVSMVVLIFYVPFLFTKEYKNNLE"
     gene            complement(1080..2042)
                     /locus_tag="PC471_003050"
     CDS             complement(1080..2042)
                     /locus_tag="PC471_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HDF2790158.1"
                     /translation="MNKSWSNLIIKNKDKKVRSGWIIIAVMATFYILQYYLSMILVEI
                     IRKILVATGDINLKTNYFSEYANWLNDVGLQIVFQILTDSLMIIIPIIVWRFIMKNPI
                     SEMGLHSYRIKKKEGYVGMLLGIFNCTVIFIITITIGGGHVTSWVPKISPLTVSWILV
                     FILVAFAEEILNRGFFMAVLRRCKNIYFIMIVPSVIFGLIHIWNPDVTFLSVINIIII
                     GILFSYMFIKSSNIWMCIGYHFAWNVFQGIIYGMPVSGLQVPGIITTQITHGNLLNGG
                     MFGIEGGILTTFVNLLSFIFVWYYYRDSKYDFVSDKVNVTNIKN"
     gene            complement(2358..3308)
                     /gene="hpdA"
                     /locus_tag="PC471_003051"
     CDS             complement(2358..3308)
                     /gene="hpdA"
                     /locus_tag="PC471_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate decarboxylase activase"
                     /protein_id="HDF2790159.1"
                     /translation="MSSQKQLEGMIFDVQSFSVHDGPGCRTTVFLNGCPLSCKWCANP
                     ESWTVRPHMMFSELSCQYENGCTVCHGKCKNGALSFNLDNKPVIDWNICKDCESFECV
                     NSCYYNAFKLCAKPYTVDELVQVIKRDSNNWRSNGGVTFSGGEPLLQHEFLHEVLLKC
                     HEVNIHTAIETSACVSNEVFNKIFNDIDFAFIDIKHMDREKHKEQTGVYNDLILENIS
                     NLANSDWNGRLVLRVPVISGFNDSVENISDIISFMHKNNLVEINLLPFHRLGESKWIQ
                     LGKEYEYSDKGDVDEGHLEELQDIFLDNGIACYVGHETAF"
     gene            complement(3301..3558)
                     /gene="hpdC"
                     /locus_tag="PC471_003052"
     CDS             complement(3301..3558)
                     /gene="hpdC"
                     /locus_tag="PC471_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate decarboxylase small
                     subunit"
                     /protein_id="HDF2790160.1"
                     /translation="MRKHSDCMNFCAVDATKGICRLSKQMINLDDAACPEIKVMPKCK
                     NCKNFVEANDEGIGKCVGLEKEDWVYSTLNAITCEGHVFNE"
     gene            complement(3562..6270)
                     /gene="hpdB"
                     /locus_tag="PC471_003053"
     CDS             complement(3562..6270)
                     /gene="hpdB"
                     /locus_tag="PC471_003053"
                     /EC_number="4.1.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate decarboxylase large
                     subunit"
                     /protein_id="HDF2790161.1"
                     /translation="MSQSKEDKIRSILEAKNIKSNFQNKENLSEFNEKKASKRAEDLL
                     DVYYNTLSTADMEFPYWYNREYRKSDGDIPVVRRAKALKAAFSHMTPNIIPGEKIVMQ
                     KTRHYRGSFPMPWVSESFFVAQGEQMREEAKKLASNTADELTKFGSGGGNVTESFGNV
                     VSIAGKFGMRKEEVPVLVKMAKEWVGKSVEDLGFHYEKMMPDYDLKENLMSTLICMFD
                     SGYTLPQGREVINYFYPLNYGLDGIIEMAKECKKAVAGNASGDGLIGMDRLYFYEAVI
                     QVIEGLQTWILNYAKHAKYLESIETDLEAKKEYSDLVEILEHIAHKQPRTFREALQLT
                     YTIHIASVNEDAISGMSIGRFGQILYPWYEQDIEKGLITKEEVIELLELYRIKITCID
                     CFASAGVNGGVLSGNTFNTLSIGGLKEDGSTGANELEELLLEASMRCRTPQPSLTMLY
                     DEKLPEDFLMKAAECTKLGSGYPAWVNNSNGTTFMMKQFADEGMTVEEARAFALGGCL
                     ETSPGCWKQLTLNGKTYSIAGGAGQSAGSGVHFIANPKILELVLMNGKDYRMNIQVFE
                     PHNKPLDTYEEVIEVFKDYYKQAINVLERANNIELDIWRKFDTSIINSLLKPDCLDKG
                     QHIGNMGYRYNATLNVETCGTVTMVNSFAALKKLVYDDKAFTIEEMKDAILNNFGFKD
                     ALEVGNYSMADQVKVDKTGKYDAIYKACLDAPKYGNNDLYADNILKNYEVWLSKVCEE
                     AQSLYAKKMYPCQISVSTHGPQGAATLATPDGRLSGTTYSDGSVSAYAGTDKNGVYAL
                     FESATIWDQAVVQNSQMNLKLHPTTIKGQQGTKKLLDLTRSYLRKGGFHIQYNVVDSE
                     TLKDAQKNPDNYRQLMVRVAGFTQYWCELGKPIQDEVIARTEYEGV"
     gene            complement(6567..7583)
                     /gene="tsaD"
                     /locus_tag="PC471_003054"
     CDS             complement(6567..7583)
                     /gene="tsaD"
                     /locus_tag="PC471_003054"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HDF2790162.1"
                     /translation="MSDIITLAIESSCDETAASVLKNGREVLSNIISTQIETHKKFGG
                     VVPEVASRKHVENIDIVVQEALDKANIGFNDIDHIAVTYGPGLVGALLVGLSYAKALA
                     YTLNIPLVGVNHIEGHLSANYIEHKDLKPPFITLIVSGGHTHLVEVKDYGKYEILGKT
                     RDDASGEAFDKISRAMNLGYPGGPIIDNLAKNGNKHAIEFPRAYLEEDSYDFSFSGLK
                     SSVLNYLNGKRMKNEEIVVEDVAASFQEAVVEVLSTKALKAVKDKGYNIITLSGGVAS
                     NSGLRAKITELAKDNGITVKYPPLILCTDNAAMIGCAGYYNFINGKTHDMSLNAVPNL
                     KINQ"
     gene            complement(7589..8065)
                     /gene="rimI"
                     /locus_tag="PC471_003055"
     CDS             complement(7589..8065)
                     /gene="rimI"
                     /locus_tag="PC471_003055"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HDF2790163.1"
                     /translation="MEDNTKLIVDDIKIEEMTTEDIDEVFEVEKNCFEDYWSKESFRK
                     ELSNEVARYIVAKLNGKIVGYVGIWLILDEGHINNVAVHSDYRGKKIGDKLIKGIVDL
                     CKDNNIASMTLEVRASNKIAQNLYRKYGFKMGGIRKEYYSDNKEDAIIMWNQLKEV"
     gene            complement(8055..8771)
                     /gene="tsaB"
                     /locus_tag="PC471_003056"
     CDS             complement(8055..8771)
                     /gene="tsaB"
                     /locus_tag="PC471_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HDF2790164.1"
                     /translation="MKILGMDTSSMAASVAVVEDDNLICEFTVNNKKTHSQKLMPMIE
                     NMLSMSDLSIKDMDLLAVCIGPGSFTGLRIGMATVKAMAHVNNIPIIAVNSLESLANN
                     INFCDRKICCILDAQKNQVYSAKYKFENGEMIELDGVDVVEFDALVDEIVSTNEEFII
                     VGEAVYKYKDKLENIKNIKIPSPANNVSNAGSLCSLALNKYNKNIDVHTCYTINPMYI
                     RKSQAEVQYDEKMKRLNNGR"
     gene            complement(8771..9223)
                     /gene="tsaE"
                     /locus_tag="PC471_003057"
     CDS             complement(8771..9223)
                     /gene="tsaE"
                     /locus_tag="PC471_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HDF2790165.1"
                     /translation="MAKIYLENENKTREIGYKLGKLLKEGSVICLVGDLGAGKTTMTQ
                     SLADSLGIEDYITSPTFTIINEYEGKIPLYHFDVYRIGSSDEMYDIGYDEYVNSNGIC
                     IIEWANLIEDILPKEYLNIELRYKDEGREMILTPKGEFYKEIVEELIK"
     gene            complement(9362..10402)
                     /locus_tag="PC471_003058"
     CDS             complement(9362..10402)
                     /locus_tag="PC471_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4116 domain-containing protein"
                     /protein_id="HDF2790166.1"
                     /translation="MHLSKKIKKLFLDDKGYYFNSNWKDIHIGIYDTKLGDSLKYITN
                     EYIENMERVKYDYRELKNIENQTDGMCILAIRQNYKALEYVKNQTEELCLEAIKYNYK
                     ALEYVKEQTEYLCLEAIKKDCNALKYVRNKTEGLIIKAISHSSNIDVVSILKALETQT
                     RRICLEAIKKDGRCLAYVREQSEELCIEAIKQNYKALKYVKNQTEKMCIESVRQNGMA
                     LQYVNKQTDKICIEAVKQDGRSIQFVNNKTEEICINAIRYLNKKYNIKDVLSYIDKYT
                     EDICIEIVRQNGKMLMYIKNQTEKMCIEAVKENYKSLKYVKEQSERICKEALKQNHKA
                     KEYVKIAIDDCI"
BASE COUNT         3373 a         1905 c         1080 g         4131 t
ORIGIN      
        1 atgaacttaa aattaatatg gataaatatt ctaaacccag tatttattaa aaatgaaata
       61 aaaagaaaaa aaatacaaat tattatcttt ttctttataa actttggttt aacttattca
      121 ttaggtttac tgttatatta taataaattt attgatccag aaaactttgc atcattcatg
      181 atgatattac ctctatcttc agttgccatt gcaaagtttt atactgaagg attaaataat
      241 gatatgtatg aattttattc actaatcata ttattttttc ttatttattc attattaatt
      301 gttcttaagt tattaaatct aataaataat aatgaattta taattataaa cctaatatta
      361 acttttataa gtagcttatc aattataata tattcatcaa ataaaaaaaa tttatatcct
      421 gtaaaaaata ttaaactagg ttctttatta attatttact ttatgatttc tgtaattgtt
      481 ccaacatcat ttatactaat actacaaggg gctcaaccta attatagtgg tatcataaaa
      541 tttatattta ctccaataac atcattaact actatttata ttttttttag tgaagaatat
      601 gcctggagag gttttttaca gaatattttt tttgataaat ttggtaaaaa gttaggagtt
      661 ataatattag gtatgtgttg gagcttgtgg cacttaccac taatatttac attatataca
      721 cctgaagcgc caatattagg aataatatta cgttcaatac atatagttgg aatttcaata
      781 tttttaggat atctttatat aaaaactaag aatatctggt tttgtgctat aattcatgtt
      841 ttaaataata gtgcttttct tatcacaaat tctaaagaat caactatcac ttaccatgat
      901 atactaatag tatctatggt tgttttaatt ttttatgttc catttctatt tacaaaagaa
      961 tataaaaata acttggagta aatagaatat ccaagttatt tttatatttt tgcaaatttc
     1021 caacaatcct acagaatact ttaagcttag aagatattat taaaatttat tataatcaac
     1081 taattcttga tattggtcac atttacttta tcactaacaa aatcatactt agaatctcta
     1141 tagtaatacc atacaaatat aaagctaagt agattaacaa aagtagtcaa aattccacct
     1201 tcaataccaa acattcctcc atttaataga ttaccatgag tgatttgtgt agttataatc
     1261 cctggaactt gaagtccact tacaggcata ccatatataa tcccctgaaa tacattccat
     1321 gcaaaatgat aacctataca catccatata ttcgacgatt ttataaacat atatgaaaaa
     1381 agaatcccaa taattataat attaattaca gacaaaaaag taacatcagg attccatata
     1441 tgaatcaacc caaaaataac agatggaaca atcattataa aataaatatt tttacatctt
     1501 cgtaaaactg ccataaaaaa accacgattt aaaatttctt ctgcaaaagc aactaaaata
     1561 aagaccagta tccaagaaac tgttaaaggt gaaatttttg gtacccatga agtaacatga
     1621 ccaccaccaa ttgttattgt tataataaag attactgtac aattaaatat tcctaataac
     1681 attcctacat atccttcttt tttcttaatt ctataagaat gtaatcccat ttcactaata
     1741 ggatttttca taataaatct ccatacaatt ataggaataa taatcataag tgaatctgtt
     1801 agaatttgaa atacaatctg taacccaaca tcatttaacc aattagcata ttctgaaaaa
     1861 tagtttgttt ttaaattaat gtcacctgta gcaaccagga tttttcttat tatttccaca
     1921 agtatcatgc ttaaataata ctgaagtatg taaaaggtag ccataacagc tataataatc
     1981 caccctgaac gtactttttt gtctttattc ttaataatca agttgctcca tgatttgttc
     2041 atatagatgt cttcctccta actattattt ttagttgttt ctaaaatata ttctattaaa
     2101 ttttttcata gtagtatttt accacaaaac ttctgcttca atgaagtaag cgtaatatat
     2161 tctctaactg taatttaaac tataaaaaat atataaagta tatttaaagc aatatttatg
     2221 gacataaagc aatataaaca atatataaaa catataaaac tttacttaat tatttctaca
     2281 taaaaaccac ataaaaaagg atgtaactct agcttttagt gtaacatcct ttttttaatt
     2341 aaaacttgta tttaatttta gaaagctgtc tcatgaccaa cataacaagc tataccatta
     2401 tccaaaaata tatcttgtaa ttcttcaaga tgaccttcat caacatcacc cttatcagaa
     2461 tactcatatt ccttgcctaa ttgaatccat tttgattctc ctaatctgtg aaatggtaat
     2521 agattaattt ctaccaaatt atttttgtgc ataaaagata taatatcact tatattttca
     2581 acactatcat taaacccaga gataactggt actctaagta ctaatcttcc attccaatca
     2641 gaatttgcaa ggtttgatat attttctaat attaaatcat tatacacacc tgtttgttct
     2701 ttgtgttttt ctctatccat atgctttata tcaataaatg caaaatcaat atcgttaaat
     2761 attttattaa aaacttcatt tgatacacat gcactcgttt caatggcagt gtgtatattt
     2821 acctcatgac actttaataa tacttcatgt aaaaattcat gctgaagtag tggctcacct
     2881 ccactaaatg ttactcctcc attacttctc caattgttag aatcacgttt aattacctgc
     2941 acaagttcat ctacagtgta aggttttgca cataatttaa acgcattata ataacatgag
     3001 ttgacgcatt caaaactttc acagtcctta cagatgttcc aatctataac tggcttatta
     3061 tcaagattaa agctcaatgc accattttta cacttaccat gacatacagt acatccattt
     3121 tcatattgac aagataattc actaaacatc atatgtggtc ttacagtcca actttctgga
     3181 tttgcacacc atttacaact cagtggacat ccatttaaaa atacagttgt tctacaacct
     3241 ggaccatcat gaactgaaaa gctttgtaca tcaaaaatca tgccttctaa ttgcttttga
     3301 ctactcatta aacacatgtc cttcacaagt aattgcattc aatgttgaat atacccagtc
     3361 ttctttctct aggccaacac atttgcctat accttcatca ttagcttcaa caaaattttt
     3421 acaattttta cattttggca ttacttttat ctctggacat gctgcatcat ctaaattaat
     3481 catttgtttt gataatctac aaattccttt ggttgcatct actgcacaaa aattcataca
     3541 atcactatgc tttctcatat tttacacccc ttcatactct gttctagcaa ttacttcatc
     3601 ttgtattggt ttacctaatt cacaccaata ttgagtaaac ccagcaacac gtaccattaa
     3661 ttgacgataa ttgtcaggat ttttttgtgc atcttttaat gtttctgaat ctacaacatt
     3721 atattgaata tggaacccac cttttcttaa ataacttctt gttaaatcta ataatttttt
     3781 agttccttgt tgtcctttta tagtagttgg atgaagtttt aaattcatct gagaattttg
     3841 aactactgct tggtcccaaa tagttgcaga ttcaaataat gcatatactc catttttatc
     3901 tgttcctgca tatgcagaga ctgaaccatc tgaatatgtt gttccactta gtctaccatc
     3961 tggagtagcc aaggtagcag ctccctgtgg cccatgtgta gaaacagata tttggcaagg
     4021 gtacatcttc ttagcatata aagattgtgc ttcttcacat acttttgata accatacctc
     4081 atagttttta agaatattat ctgcatacaa atcattattt ccatatttag gtgcatcaag
     4141 acaggcctta taaatagcat catatttacc agtcttatct acttttactt ggtctgccat
     4201 agaataattt cctacttcta atgcatcttt aaatccaaaa ttatttaata tggcatcctt
     4261 catttcctct attgtaaatg ctttgtcatc atatacaagt ttcttaagtg ctgcaaatga
     4321 attgaccata gtaacagtac cacaagtttc tacgtttaat gttgcattat aacgatatcc
     4381 catatttcct atatgttgac ctttgtctaa acagtctggc tttagtagtg aatttattat
     4441 agaagtatca aattttctcc atatatctaa ctcaatgtta tttgctcttt ctaaaacatt
     4501 tattgcttgt ttataataat ctttaaatac ttctatgact tcttcataag tatctaatgg
     4561 cttattatga ggctcaaata cttgtatatt catacgatag tctttaccat tcataagaac
     4621 aagttctaaa atttttggat tagctataaa gtgaactcca gagccagcag attgacctgc
     4681 tccacctgca atagagtatg tttttccgtt taaagttaac tgtttccaac accctggaga
     4741 cgtttccaaa catccaccta atgcaaatgc tcttgcttct tcaacagtca taccttcatc
     4801 agcaaattgt ttcatcataa atgttgttcc atttgagttg tttacccatg ctggataacc
     4861 agaaccaagc tttgtacatt cagcagcttt cattaagaaa tcttctggaa gcttttcatc
     4921 atatagcata gttaaactag gttgaggagt tctacatctc atacttgctt ctaataataa
     4981 ctcttctagt tcattagctc ctgtagaacc atcttctttt aatccaccta tagataaagt
     5041 attaaatgta ttgcctgata gtactcctcc atttacgccc gctgaagcaa aacagtcaat
     5101 acaagtgatt tttattctat aaagctctaa taattcgata acctcttctt ttgtaattag
     5161 tcctttttct atatcttgtt cataccaagg atataaaatt tgaccaaaac gtcctataga
     5221 cataccagat atagcatcct catttactga tgctatatga attgtataag tcaactgaag
     5281 agcttcccta aatgtacgag gttgtttatg tgcaatatgt tctaatatct ccactaaatc
     5341 tgagtattct tttttagctt ctaaatctgt ctctatactt tctaaatatt ttgcatgttt
     5401 tgcataattt aaaatccaag tttgtaatcc ttcaataact tgaataacag cctcataaaa
     5461 atatagacgg tccataccaa taagcccatc tccagatgca tttcctgcaa ctgccttttt
     5521 acattctttt gccatttcta taattccatc taatccataa tttaaaggat aaaaataatt
     5581 tataacttct ctaccttgag gaagtgtata ccctgaatca aacatgcata taagtgtact
     5641 cattaggttt tcttttaaat cataatctgg catcattttt tcatagtgaa aacctaaatc
     5701 ttcaacagac tttccaaccc attcctttgc catttttaca agtacaggta cttcttcttt
     5761 tctcattccg aatttaccag ctattgaaac gacatttcca aaactttcag taacatttcc
     5821 tccaccagac ccaaattttg taagttcatc agctgtgtta ctggctaatt tctttgcctc
     5881 ttctctcatt tgttctcctt gagcaacaaa gaagctttca cttacccatg gcattggaaa
     5941 agaacctcta taatggcgtg tcttttgcat tactattttc tcaccaggta ttatatttgg
     6001 tgtcatatgt gaaaaagcag ctttaagagc tttagctctt ctaactactg gtatatcacc
     6061 atctgatttt ctatattctc tattatacca atatggaaat tccatatcag cagtagataa
     6121 tgtattataa tatacatcta ataagtcttc tgctctttta cttgcttttt tttcattaaa
     6181 ttcacttaaa ttttctttat tttgaaaatt tgatttaata ttttttgctt ccaaaatact
     6241 tcttattttg tcttctttac tttgactcat ttcttcccct ccttaatctt tctataatta
     6301 gtataaaact ttttaagaat gaaaaaaaag ggtatattac ccacttttta aaataatctt
     6361 tctatagaaa aaaataaact tgaagcaaat ctaagccaaa gacaatctgt atatcctaaa
     6421 atttgttgca attcattgga atataaagat tataaatagt tttacttcaa gttttaatat
     6481 atactatatt tatttttatt atatttaatt tcacttctac ttactttttt gaaacaatac
     6541 ttttatattt acaaattcat ctaactctac tgatttatct ttaaatttgg aactgcattt
     6601 aaactcatat catgcgtttt cccatttata aaattataat atcctgcaca ccctatcata
     6661 gctgcattat ctgtgcataa aattagagga ggatacttaa cagtaatacc attgtcttta
     6721 gcaagctctg ttattttagc tctaagcccc gaattagaag caaccccacc agaaagagtt
     6781 atgatattgt atcctttgtc ttttactgct tttagtgctt tagtagataa aacctctact
     6841 acagcctctt ggaaagatgc tgctacatct tcaactacta tttcttcatt tttcattctt
     6901 tttccattta gataatttaa tacagaagat tttaatccac taaaactaaa atcatagcta
     6961 tcttcttcta aataggctct tggaaattct atagcatgtt tatttccatt cttggccaga
     7021 ttatcaataa taggacctcc tggatagcct aaattcatag ctcttgaaat cttatcaaat
     7081 gcttctccag atgcatcatc tcttgttttt cctaaaattt catacttgcc ataatctttt
     7141 acttcaacca agtgagtatg acctccagat actattaatg ttataaaagg aggttttaaa
     7201 tccttatgct ctatatagtt tgcacttaga tgcccttcta tatgatttac tcctacaagt
     7261 ggaatattca aagtatatgc aagtgcttta gcataagata gaccaaccaa taaagcacct
     7321 actagcccag gtccatatgt aactgcgata tggtctatat cattaaaacc tatatttgct
     7381 ttgtctaaag cttcttgaac aactatatca atattttcca catgttttct tgaggccact
     7441 tctggaacta caccaccaaa ttttttatgt gtctcgattt gagtagagat tatgtttgaa
     7501 agtacttctc taccattttt taaaactgat gcagctgttt cgtcacaact actctctata
     7561 gctaatgtta ttatatcact cataaactct acacctcttt taattggttc cacattataa
     7621 ttgcatcttc tttattatca ctgtagtatt cttttcttat tccacccatt ttaaatccat
     7681 attttctata taaattttgt gctattttat ttgatgctct aacttctaat gtcatagatg
     7741 caatattatt atctttacat aaatcaacta tgccttttat caacttgtcc cctatttttt
     7801 tccctctata gtcactatga acagccacat tatttatatg cccttcatca agtataagcc
     7861 aaattcccac atatccgaca atttttccat ttaacttagc tactatatac cttgctactt
     7921 cattagacaa ctcttttcta aaagactctt ttgaccaata atcttcaaaa caattttttt
     7981 ctacttcaaa aacttcatct atatcttctg tagtcatttc ttctattttt atatcatcta
     8041 ctattagttt tgtattatct tccattattt agcctcttca ttttttcatc atattgtact
     8101 tcagcttgag attttctgat atacatagga tttatcgtat aacaagtatg tacatctata
     8161 tttttattat atttatttaa tgctaaagag cataagcttc ctgcgttaga tacattatta
     8221 gctggtgaag gtatttttat attttttata ttctctaact tgtctttata tttgtataca
     8281 gcttctccaa ctattataaa ttcttcatta gttgatacaa tttcatctac taaagcatca
     8341 aactctacaa catcaacacc atctaattct atcatctctc cattttcaaa cttgtattta
     8401 gctgaataaa cttgattttt ctgtgcatct aatatacaac atatctttct atcacagaaa
     8461 tttatgttat ttgctagaga ttccaaagaa tttactgcta ttattggtat attatttaca
     8521 tgggccatag cttttactgt agccatacct attctaagtc ctgtaaacga acctggacct
     8581 atacatacag ctaataaatc catatctttt atacttaaat cactcatact caacatattt
     8641 tctatcattg gcataagctt ttgtgaatga gtttttttgt tgtttactgt aaactcacat
     8701 attaagttat catcttcaac aactgctaca cttgctgcca tagaagacgt atccatacct
     8761 agtattttca ttatttaatc aactcctcaa ctatctcttt ataaaactct ccttttggag
     8821 ttaatatcat ttctcttcct tcatctttat atctcaattc tatattcaaa tattcttttg
     8881 gaagtatatc ttctattaga ttagcccatt ctattataca gattccattt gaatttacat
     8941 attcatcata accaatatca tacatttcat cactactacc tattctataa acatcaaaat
     9001 gatacaaagg tatttttcct tcatattcat ttattatagt aaaagtagga cttgttatat
     9061 aatcttctat ccctaatgaa tctgccaaac tttgtgtcat tgtagtttta ccagctccta
     9121 aatctcctac taaacagata acactgcctt cttttaaaag tttacctaat ttatatccta
     9181 tttctctagt tttattttca ttttctaaat atatttttgc catcaattca tctccatttt
     9241 gatataatcg tcatgtgaat ttatatcact gcttaattaa ttatagtata agcctagatt
     9301 attataaagt acttgtcttt atcttttttt atctttcact attgatggct gtatctacac
     9361 cttatataca atcatcaata gcaattttta catactcttt tgctttatgg ttttgcttca
     9421 atgcttcttt acatattctc tcagattgct cttttacata cttaagagac ttatagttct
     9481 cttttactgc ttcaatacac attttttctg tttgattttt tatatacata agcatttttc
     9541 cattttgtct tacaatctct atacatatat cttctgtata cttatctatg taacttagta
     9601 catctttaat attatatttt ttatttaaat atcttatagc atttatacat atttcttctg
     9661 tcttattgtt gacaaattgt atacttcttc catcttgttt gacagcttct atacatattt
     9721 tatcagtttg tttatttaca tattgtaatg ccataccatt ttgtcttacg gattctatac
     9781 acattttttc cgtttgattt tttacatatt ttaatgcttt ataattttgc ttaatggcct
     9841 ctatacataa ttcttcactc tgttctctaa cgtatgcaag acacctgcca tcttttttaa
     9901 ttgcttctaa acatatcctt cttgtttgag tctctaaagc ctttagtata cttacaacat
     9961 ctatattgga agaatgactt attgctttta taataagccc ttctgtttta tttcgtacat
    10021 acttaagtgc attacaatct ttttttatag cttcaagaca caagtattct gtttgttctt
    10081 ttacatattc aagtgcttta taattatact ttattgcttc taaacataat tcttcggttt
    10141 gattttttac atactctaat gccttgtaat tctgccttat tgctaaaata cacataccat
    10201 cagtttgatt ttctatattt tttaattcac gataatcgta ttttaccctt tccatatttt
    10261 caatatattc attagtgata tactttaaag aatctcctaa ttttgtatca taaataccaa
    10321 tatgaatatc tttccaatta ctattaaaat aatacccttt atcatctaaa aataattttt
    10381 ttattttttt agataaatgc atgaatatac acccctatca ttattaaatt agttattatt
    10441 aaaagtttga ttattaatct ttatagtttt ttaataataa ctttatttt
//
LOCUS       DAMJYR010000124        10331 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.129, whole genome shotgun sequence.
ACCESSION   DAMJYR010000124 DAMJYR010000000
VERSION     DAMJYR010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10331)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10331)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10331
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.129"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            <1..1164
                     /locus_tag="PC471_003059"
     CDS             <1..1164
                     /locus_tag="PC471_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated protein Cse4"
                     /protein_id="HDF2790167.1"
                     /translation="QKYLYPNIYNKNDFNVIIDGKNYGLPNDNMQLNEKKPFLKLKTR
                     IRNSTPYLISDDEVLLQKAFFDYLTNKAANGEEVIYISSEKGIHRKTDIPNEFSGYIL
                     RIKKGKELEIHDFDTVVNLNTKIKRINVECIIPIEYERHNLKPNLEIGYINNLIKLEK
                     TIDTVFFNKFMCNSYFKEIKNINLSDLVLRDILIRHREGLYTWFYKGRDSIVKQTFSN
                     YSMGIIENSIKNNNYIRAKEQYNLRIAIINYFHGGSNMADKIKVISESLFEKINSIKT
                     KCIQSDEEYYFAVGQLASYFISKNKSKNKNHSLINPILNAQNRKKIMLEIEKLFKKYN
                     YDISYDNNRRFKNLYSMIVGYEVEEKKVNRDLLIGGYLYYNLIYTKSEENENE"
     gene            1151..2146
                     /locus_tag="PC471_003060"
     CDS             1151..2146
                     /locus_tag="PC471_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I CRISPR-associated protein Cas7"
                     /protein_id="HDF2790168.1"
                     /translation="MKMNRRVYGVLGIKSIMSNWNADFSGYPKTTSTGEVFGSDKALK
                     FPMKKMWENEGEKVLYVKSLKIDESKKGEIKIRPKSLKERYEELFDIEDLKLEKNQQK
                     VLENLFKAIDVKNFGATFAEEGNNIGIMGAVQIGQGFNKYEDINVEEQQILSPFRDPK
                     AKDKKKSTNEEEKEDANSTTLGKKIVSDEAHYFYPFSINPKAYEVFVNMGVTDGYTKN
                     DYKKFKEAALVSATAFNTNSKLGCENEFALFVETDEELYLPDLAQYITFEKREKSKDL
                     ICINIIHLIAELKDDIKSVEIYYNPYKIEIKPFKDEILPKIDKVKFMNIFTRKEV"
     gene            2148..2888
                     /gene="cas5b"
                     /locus_tag="PC471_003061"
     CDS             2148..2888
                     /gene="cas5b"
                     /locus_tag="PC471_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-B CRISPR-associated protein Cas5"
                     /protein_id="HDF2790169.1"
                     /translation="MEVLKFNLSGKTAFFKKPDVNSIIYFTYGNIHKIALMGILGACL
                     GLGGYNQQSKEDEYPEFYSLLKDIKMSIVPKHEFGYINKKVHIFNNSVGYASKEEGGN
                     LIIKEQWLENVSWDIYIQLEDSTTSRELKRRFLSNKFVYIPYLGKNDHIANISEIEII
                     TDFYKTEDINRVDSFFIREDFEFEKSYFDDNDDYKEPFKYQEKLPYRLHRMTNQYILE
                     TIIFTNMSVKLINKKEVYQVKGLNLIFI"
     gene            2912..5470
                     /locus_tag="PC471_003062"
     CDS             2912..5470
                     /locus_tag="PC471_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated helicase/endonuclease Cas3"
                     /protein_id="HDF2790170.1"
                     /translation="MYFKKVELFDISNNINNCKNIYAHINKDRKPETLKEHSDLSYSY
                     LMKLCVKKDLSSCLERIENKFFKKDSFLSKNFYREIILNMVYMHDIGKINSSFQFVKM
                     QNKNFKKISLRYTKHSFISSMLYFDYYFEKLKKYDFEEKKILSLFLMINTYTISKHHG
                     VLKSFKEFLFLFKDEFINIKENFKDLFKNNYNLELKSVPKTIEKMVRGVEAYLDDSHI
                     KDNLAIDLYIYSKLMFSIVTSCDFYATSEYNSGNSIDDFGTIDNYNKYYDVLKKSEIY
                     KGMKKYREYLNNNSECPYKENDINRLRTEMSIEAEDAITHNTRKRIFYLEAPTGSGKT
                     VTSINLALKSIELNQSLKKIFYIFPFNTLVEQTKDVFINEIFNSVKDIQKDVVVINSI
                     TPIQTEDKEEDNKNVEYCVTKQEIDYDKSLLNRQFLHYPIVLTTNIGFFNYLFGVSRE
                     ECFPLIHMINSVIILDEIQNYKNEIWKEIILFLEKYADILNIKFIIMSATLPRLNKLG
                     CNDDNFAYLVKNREKFFKNHLFKDRVNISFEMLNSEINDIEEISEKVIEEAEIYNKIL
                     IEFIKKSSALKFYKNIKEKLKYKLEDVFLLTGDDNKLERKKIINKIKDMNSVILVATQ
                     VVEAGVDIDMDLGFKDISTIDSDEQFLGRINRSCKKLNSKVYFFNLDDASKIYKKDVR
                     KERHLTLNEESNRKIILDKDFEKYYDKIIARIEENKHKHNDKNIEVFIKDIVGNLNFT
                     EVKKWMALIPDLKEYTIFLNTIVKDESGNMIVGSDVWYEYISLLKNDNVEYSEKRVKM
                     SEIMEKLDYFTYKVQKFDNSFNDLVGDIFYIDDGSKYFTEGKFDRSKFNQNEFL"
     repeat_region   5660..8661
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=5660..5689
                     /rpt_unit_seq="gttaaacagtaacatgagatgtatttaaat"
     gene            8746..9240
                     /locus_tag="PC471_003063"
     CDS             8746..9240
                     /locus_tag="PC471_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated protein Cas4"
                     /protein_id="HDF2790171.1"
                     /translation="MKITGTLINYYFHCKRQCWLLGNRINLEENSEDVKIGRLLHELK
                     EEKTKYKEIAIENIKIDKLTREYLVEVKKSDADIEAVKWQVLLYLKKLKEKGIVRKGK
                     IEFIEKNKTNKKIVFVDLSEVSEEQLISIEREIVNLINSDKAPCPDLKKGCKKCAYYE
                     YCYI"
     gene            9275..10273
                     /gene="cas1b"
                     /locus_tag="PC471_003064"
     CDS             9275..10273
                     /gene="cas1b"
                     /locus_tag="PC471_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-B CRISPR-associated endonuclease Cas1"
                     /protein_id="HDF2790172.1"
                     /translation="MGKTKYITSMGELTRKDNSICFRKNGRNVYIPVENTSEIFCLNE
                     VSLNSKLLDFLSRANIVVHFFNYYGGYSGTFYPKEYLISGKVTVAQVKTFEDRREEIA
                     KSIVNGIRINAINTLSHYYKHGNSEIQPLLSHLKKDSINKLEKSNNIKEILSVEGDIW
                     QTFYTSFKYILREEFIFNKRVKRPPDNPLNAMISFGNSILYAKTVTAIYNTHLNQSVS
                     FLHEPSESRFSLSLDLSENFKHVITFKTIFDLVNNRKINIEKHFDKNLNYCVLNDEGK
                     QIFINSLEERLESVKEHPKLKRKVSLKTCIKLDAYKMLKCIMEEKSFIPYNIKDGL"
BASE COUNT         4423 a          951 c         1585 g         3372 t
ORIGIN      
        1 caaaaatatt tatatccaaa tatatataat aaaaatgatt tcaatgtaat tattgatggt
       61 aaaaactatg ggttaccaaa cgataatatg caattaaatg aaaaaaagcc atttttgaaa
      121 ttaaaaacaa gaattagaaa ttcaactcct tatttgatta gtgatgatga agttttgtta
      181 cagaaagctt tttttgatta tctaacgaat aaagcagcga atggagaaga agtaatatat
      241 ataagtagcg aaaagggaat tcatagaaaa actgatatac caaatgaatt tagtggttat
      301 atactgagaa taaaaaaggg taaagaatta gaaatacatg attttgatac tgtggtaaat
      361 ttaaacacta aaataaaaag aataaatgta gaatgtataa taccaataga atatgaaaga
      421 cataatttaa aaccaaattt agagatagga tatattaata atcttataaa attggaaaaa
      481 acgattgata ctgtattctt taataaattt atgtgcaata gttattttaa agaaattaaa
      541 aatataaact taagtgattt agttctaagg gatatactta ttagacatag agaaggttta
      601 tatacctggt tttataaagg gagagatagt atagtaaagc aaactttttc aaactattct
      661 atgggtataa ttgaaaattc tataaaaaac aataactaca taagagcaaa ggagcaatat
      721 aatttaagaa tagctataat aaattatttt catggaggaa gtaatatggc agataaaata
      781 aaagttatta gtgaatcttt atttgaaaaa ataaattcta taaaaacgaa atgtatacaa
      841 agtgatgaag aatattattt tgcagttggg caattagcat cgtattttat atctaaaaat
      901 aagtcaaaaa ataaaaatca ttctctgatt aatcctatac taaatgcaca aaatagaaaa
      961 aaaatcatgt tagagataga aaaattattt aaaaaatata attatgatat aagttatgac
     1021 aacaataggc gatttaagaa tctatattct atgattgtag gatatgaggt agaagaaaaa
     1081 aaagttaata gagatttatt aattggtgga tatctatatt acaatttaat atacacaaaa
     1141 agtgaggaga atgaaaatga atagaagagt atatggagta ttaggtataa aatctataat
     1201 gtcaaactgg aatgctgatt tttctggata tccaaagacg acatcaacag gagaagtttt
     1261 tgggagtgat aaagcactta agtttcccat gaaaaaaatg tgggaaaatg aaggtgaaaa
     1321 agttttatat gttaaatcat taaaaataga tgaatctaag aaaggagaaa taaaaattag
     1381 accaaaatca ttaaaagaga gatatgaaga attgtttgat atagaggacc taaaactaga
     1441 aaagaatcaa caaaaagtac tggaaaatct atttaaagct attgatgtta aaaattttgg
     1501 tgcaacattt gcagaagaag gaaataatat aggaattatg ggcgcagtac aaattggaca
     1561 aggatttaat aagtatgaag atattaatgt tgaagaacaa caaatattat ccccatttag
     1621 ggatccaaaa gcaaaagata agaaaaagtc aacaaatgaa gaagaaaaag aagatgcaaa
     1681 ttcaactaca ttaggaaaaa aaatagtaag tgatgaggct cattactttt atccattttc
     1741 aataaatcca aaagcttatg aagtttttgt taatatgggt gtgactgatg gatatactaa
     1801 aaatgattat aaaaaattta aagaagctgc tttagtaagt gcaactgcat tcaatacaaa
     1861 ttcaaagctt ggttgcgaaa atgaatttgc attatttgtt gaaactgatg aagagttata
     1921 cctaccagat ttggcacaat atataacttt tgaaaagaga gaaaaatcaa aagatttaat
     1981 ttgtataaat ataattcatc ttatagcaga attaaaagat gatataaaga gcgttgagat
     2041 atattataac ccatataaaa tagaaataaa gccttttaaa gatgaaatat tacctaagat
     2101 agataaagtt aaatttatga acatatttac aagaaaagag gtataatatg gaggttttga
     2161 aatttaactt aagtggaaaa actgcttttt ttaaaaaacc agatgtaaat agcataattt
     2221 attttacgta tggaaacata cataaaattg cacttatggg tattttagga gcttgtttag
     2281 gacttggtgg atataatcaa cagagtaagg aagatgagta tccagaattc tactctttac
     2341 ttaaggatat aaaaatgtca atagttccaa agcatgaatt tggatatata aataaaaaag
     2401 tccatatatt taataattct gtaggatatg catcaaaaga agaaggtgga aatttaataa
     2461 taaaagaaca gtggcttgaa aatgttagtt gggatatata tatacaatta gaagattcaa
     2521 ctacaagtag agagcttaaa agaagatttt tatctaataa atttgtatat ataccttact
     2581 taggcaaaaa cgatcatata gctaatataa gtgaaataga gataattact gatttttaca
     2641 aaacagaaga tattaacaga gtggacagtt tctttataag agaggatttt gagtttgaaa
     2701 aatcttattt tgatgataat gatgattata aagagccatt taaatatcag gaaaaacttc
     2761 cttatagact gcaccgaatg actaatcaat atattttgga aactatcata tttactaata
     2821 tgtcagttaa attgattaat aaaaaagagg tatatcaagt aaagggttta aatttaatct
     2881 ttatttaaat ttataggttt aggagaatta tatgtacttt aaaaaagttg agttgtttga
     2941 tataagtaat aatataaata attgcaaaaa tatttatgct catataaata aagacaggaa
     3001 gccagaaact ttaaaagaac attcagactt atcatactca tatcttatga aattatgtgt
     3061 aaaaaaagat ttaagctctt gcctagagag aatagagaat aaatttttta aaaaagattc
     3121 ttttttaagt aagaattttt atagagagat tatcttgaat atggtttata tgcatgatat
     3181 aggtaagatt aattcttcat ttcagtttgt aaaaatgcaa aataaaaatt ttaaaaaaat
     3241 ttcacttaga tatactaaac attcttttat ctcttcaatg ctatattttg attactattt
     3301 tgaaaagctt aaaaaatatg attttgaaga aaaaaaaata ttgagtttat ttttgatgat
     3361 aaatacatat acaatatcaa aacatcatgg ggttctgaag tcatttaaag aatttttatt
     3421 tttatttaaa gatgagttta taaacattaa agaaaatttt aaagatttat ttaaaaacaa
     3481 ttataattta gagcttaaat cagttcccaa aactatagaa aaaatggtaa gaggtgtaga
     3541 agcgtattta gatgatagcc atataaagga taacttagct atagacttat atatatattc
     3601 aaaattgatg ttttcaattg ttacatcttg tgatttttat gctacatcag aatataatag
     3661 tggtaattct atagatgatt ttgggacaat tgataattat aataagtatt atgatgtatt
     3721 aaaaaaatct gaaatatata aggggatgaa aaaatatagg gaatacttaa ataacaatag
     3781 tgaatgccca tataaagaga atgatataaa cagattgaga acagaaatga gtatagaagc
     3841 tgaagatgca attacacata atacgagaaa aagaatattt tatttagaag caccaactgg
     3901 aagtggaaaa actgttacat ctataaatct agcactgaaa tctatagagt taaatcaatc
     3961 tttaaaaaag atattttata tatttccatt taacacatta gtagagcaaa caaaagatgt
     4021 atttataaat gaaatattta attctgttaa agacatacaa aaagatgttg tagttataaa
     4081 ttcaataaca ccaatacaaa cagaagataa agaagaagat aataaaaatg ttgaatattg
     4141 tgttactaaa caagaaattg attatgataa atcattgtta aatagacaat ttttacacta
     4201 tcctatagta cttacaacta atataggttt ttttaactat ttgtttggtg tatcaagaga
     4261 agaatgtttt ccattgatac atatgataaa tagtgtaatt atattagatg aaatacaaaa
     4321 ttataaaaat gagatatgga aagaaattat tttgttttta gaaaaatatg cagatatatt
     4381 aaatataaaa tttattataa tgtctgcaac tttacctaga ttaaataaat taggatgtaa
     4441 tgatgataat tttgcatatc tagtaaaaaa cagagaaaaa ttttttaaaa atcacctttt
     4501 taaagataga gtaaacataa gttttgagat gttaaattca gaaattaatg atattgaaga
     4561 aatatcagaa aaggttatag aagaagcaga aatatataat aagatactga tagagtttat
     4621 aaagaaatca tcagcactaa aattttataa aaatattaaa gaaaaattga agtataagtt
     4681 agaagatgtt tttttattaa caggtgatga taataaatta gaaagaaaaa aaataataaa
     4741 taaaattaaa gatatgaact ctgtaatttt ggttgctaca caagttgtag aggctggagt
     4801 agatatagat atggatttgg gatttaaaga tatatctaca attgattctg atgaacaatt
     4861 tttgggaaga ataaatagat cttgtaaaaa attaaattca aaagtatact tttttaattt
     4921 agatgatgca agtaagatat ataagaaaga tgttagaaaa gagagacatt taactcttaa
     4981 tgaggaaagt aatagaaaaa ttattttaga taaagacttt gaaaagtatt atgataaaat
     5041 aatagccaga atagaagaaa ataagcataa acataatgac aagaatatag aggtttttat
     5101 aaaagatatt gtgggaaatt tgaattttac agaggtaaaa aaatggatgg ctttaatacc
     5161 agatttaaaa gagtatacaa tctttttaaa tactatagtt aaagatgaat ctggaaatat
     5221 gattgtagga agtgatgtat ggtatgaata tatatcacta ctaaaaaatg ataatgttga
     5281 gtattctgaa aagagggtga aaatgtctga aattatggaa aaactagatt attttacgta
     5341 taaagttcag aaatttgata attcatttaa tgatttagtt ggagatattt tttatattga
     5401 tgatggaagt aagtatttta cagagggaaa gtttgataga agtaaattta atcaaaatga
     5461 atttctataa tgtatttaga ttatatagag aatatttatc ccaagttaat taattagaat
     5521 tagtttaaaa ctattgaaat atagatattt tatagcttat atattttttc taggattagc
     5581 tgggataaaa aatctgtata ttagtgtttt cagtgtattt aaggggatta tatttttaag
     5641 aagtactgat tttaaagtgg ttaaacagta acatgagatg tatttaaatt tttactacat
     5701 aattaaatcg tagtcaatac cgtacaggtt aaacagtaac atgagatgta tttaaatctc
     5761 tgaatagcca atcaactttg ctgcattact cttgttaaac agtaacatga gatgtattta
     5821 aatgataata gttatatata ttttttacaa tatattattg ttaaacagta acatgagatg
     5881 tatttaaatt tacttctcct gttttgaaat ctatatattc atcatgttaa acagtaacat
     5941 gagatgtatt taaatataat attgatacaa atattttggg taaatggact tgttaaacag
     6001 taacatgaga tgtatttaaa ttcgatatgg taacaaattt ataaaagata tagaatgggt
     6061 taaacagtaa catgagatgt atttaaataa agaaaaacga aattggtgat tttgaagcaa
     6121 tgtgttaaac agtaacatga gatgtattta aattagtaac tacaatgctt ttccttttga
     6181 agatgatatg ttaaacagta acatgagatg tatttaaatt aataatgcac ctattttaac
     6241 agtacctggt ctaaagttaa acagtaacat gagatgtatt taaattcaag tctaggagac
     6301 aacataaata gtacagatgg gttaaacagt aacatgagat gtatttaaat tagtaaaacc
     6361 agatggaact gttgtaatac caccaaagtt aaacagtaac atgagatgta tttaaattta
     6421 aaaacatctt gaggaatatc tttaacagta attgttaaac agtaacatga gatgtattta
     6481 aatgcatttt caggtgcatg ataaataggt aaattagatg ttaaacagta acatgagatg
     6541 tatttaaatt ttttgtaatt agccagatta tcaactccca aaatagttaa acagtaacat
     6601 gagatgtatt taaattaaat atcgtccttc gtagtcaaag caacaccaga aggttaaaca
     6661 gtaacatgag atgtatttaa atcgtaaaca aaatttatca agagaagaat ttctaaggtt
     6721 aaacagtaac atgagatgta tttaaattta tatttgataa gagtatcaaa tcgacttaaa
     6781 atagttaaac agtaacatga gatgtattta aattcaatct taatacacag acatttttaa
     6841 aaacaaaacg ttaaacagta acatgagatg tatttaaata aatagacaaa tattgaatag
     6901 ctctgtcttc atcaagttaa acagtaacat gagatgtatt taaatatatt tacaaaatct
     6961 ctaacatatt ttttaacttc tgttaaacag taacatgaga tgtatttaaa ttcttttaaa
     7021 gagtcctcgg gagcctcgaa aacttcagtt aaacagtaac atgagatgta tttaaattac
     7081 tcaaataaat ttctaatttg tttatctact aacgttaaac agtaacatga gatgtattta
     7141 aattagaatt tacaacaata taagcctctt taaccataag ttaaacagta acatgagatg
     7201 tatttaaata tgtttttagt agtacattta ataaaacttt ttaaagttaa acagtaacat
     7261 gagatgtatt taaatgaagg aaccgtacct tgataaatca cgaccgtacc cgttaaacag
     7321 taacatgaga tgtatttaaa ttgtatattt gatgttgtta tttctcctgt gtataatgtt
     7381 aaacagtaac atgagatgta tttaaatgaa gttccacccc caccaccccc actactttga
     7441 gaagttaaac agtaacatga gatgtattta aattaacaat agagtaaata taataaacaa
     7501 gcatacaaag ttaaacagta acatgagatg tatttaaatc cccttttgca tggtactaga
     7561 cacgcatata tggtggttaa acagtaacat gagatgtatt taaatacgcc tatatgcgga
     7621 gtaacagatt tttgcctgga tggttaaaca gtaacatgag atgtatttaa atactgtttt
     7681 gggatagact ggaattaaat ggtctgtgtt aaacagtaac atgagatgta tttaaatttt
     7741 tggaatgaag catcaacaga taataaagaa aaagttaaac agtaacatga gatgtattta
     7801 aatcaaggaa acaaaagaga agtgaaaaga aatataatgt taaacagtaa catgagatgt
     7861 atttaaataa tcttttaaat ggaaattaag taataaaaga gagggttaaa cagtaacatg
     7921 agatgtattt aaattcaatt aaataagcat taatcttagt aaaatcaaac gttaaacagt
     7981 aacatgagat gtatttaaat tttcttgtgt taattgaacc cccattttaa gctccagtta
     8041 aacagtaaca tgagatgtat ttaaatcaga atacagcttc tatatctgga tacatttgtc
     8101 ttggttaaac agtaacatga gatgtattta aatgtgaaaa gtaatacagg tatagagtct
     8161 aaacgctcgg ttaaacagta acatgagatg tatttaaatc aatgtatcct cttttttagt
     8221 tttggagcct cttttgttaa acagtaacat gagatgtatt taaattagtt agcacatttt
     8281 atttcaagcc ttaaaacatt cgttaaacag taacatgaga tgtatttaaa ttaatttgca
     8341 cacttaacct ctaatcttaa agtattagtt aaacagtaac atgagatgta tttaaataac
     8401 acaatattat ctttaataga attaaacaaa tgagttaaac agtaacatga gatgtattta
     8461 aatttttgtt ggtcttgtgc ttcttttaaa agctgattag ttaaacagta acatgagatg
     8521 tatttaaatt taaaaagttc tgttattaaa acatccaaac caatcgttaa acagtaacat
     8581 gagatgtatt taaattctgt accatctagc caaaccgttt ttgaagttaa agttaaacag
     8641 taacataaga tgtatctaaa ttactctatc ataaaatttt aatatattaa acaaactaat
     8701 atgtgatata cttatgtata aataacaaaa taaataggtg atgttatgaa aattacagga
     8761 actttgataa attactattt ccattgtaaa agacaatgct ggttgcttgg taatagaata
     8821 aatttggaag aaaatagtga agatgtaaaa ataggtagac ttcttcatga actaaaagaa
     8881 gaaaaaacaa aatataaaga aattgcaatt gaaaatataa aaatagacaa attaacaaga
     8941 gaatatttag tagaggttaa aaagtctgat gctgatatag aagctgttaa gtggcaagtt
     9001 cttttatatt taaaaaagtt aaaagaaaag ggaattgtaa gaaaagggaa aattgaattt
     9061 atagaaaaaa ataaaactaa taaaaaaata gtctttgtag atttgagtga agtatctgaa
     9121 gaacagctta tatctataga aagagaaatt gttaatttaa taaatagtga taaagcacca
     9181 tgtcctgatt taaaaaaagg atgtaaaaaa tgtgcctatt atgaatattg ttatatataa
     9241 agaggtataa gaaacatata aggaggtgtt aatattggga aagacaaaat atattacctc
     9301 aatgggtgaa ctcacaagaa aagataattc tatatgtttt agaaaaaatg gaaggaatgt
     9361 atatatacca gtagaaaata ctagtgaaat attctgtcta aatgaggtat ctttaaattc
     9421 aaaattactg gattttttat caagagcaaa tattgttgtt cattttttta attactatgg
     9481 tggatatagt ggaacttttt atcctaaaga atatttaata agtggaaaag taacagtggc
     9541 acaggtaaaa acctttgaag atagaagaga agaaatagca aaatccatag tcaatggtat
     9601 aaggattaat gcaataaata cattatcgca ttactataag catggaaatt cagaaataca
     9661 accattatta tcacatctaa aaaaagattc tataaacaag ttagaaaaat caaataatat
     9721 aaaagaaata ttatcagtcg aaggagatat ttggcaaaca ttttatactt cctttaagta
     9781 tattttaaga gaggaattta tttttaataa aagagttaag agaccaccag ataatccctt
     9841 aaacgcaatg atatcatttg gtaacagtat tttatatgct aaaactgtaa ctgctatata
     9901 taatacacat ctaaatcaaa gtgtaagttt tttacatgaa ccaagtgaaa gtagattttc
     9961 tttaagtcta gatttaagtg aaaattttaa acatgttata acttttaaaa ctatatttga
    10021 tttagttaat aatagaaaaa taaatattga aaaacatttt gataaaaatt tgaactactg
    10081 tgttctaaat gatgaaggaa agcaaatttt tataaattct ttagaagaaa gattagaatc
    10141 agtcaaagaa catccaaaat taaaacgaaa agtaagttta aaaacctgta taaaattaga
    10201 tgcttacaaa atgttaaagt gtataatgga agaaaaaagt tttattccat ataacataaa
    10261 agacggatta taatttaaac ataaagtaag tattttaata tagaactttg atatatataa
    10321 gaatgataat t
//
LOCUS       DAMJYR010000125        10252 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.130, whole genome shotgun sequence.
ACCESSION   DAMJYR010000125 DAMJYR010000000
VERSION     DAMJYR010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10252)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10252)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10252
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.130"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            <1..922
                     /locus_tag="PC471_003065"
     CDS             <1..922
                     /locus_tag="PC471_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HDF2790173.1"
                     /translation="NSIYFVPLILDISFLIKEKYKYILFSFVIISSLIISLDKNIYLA
                     LESSSILLIITVLSIYIENENLSKLYSQNLYDKLRISKDELKKVNADLETYASSIEEL
                     AILKERNRISREIHDSVGHSLSTTIIQLNAIEKLLKDKPLIYDLVHELREFVTESFQD
                     VRRAISELKPVEYENYQSLFKIKELVKSFIKLTNINVKLTISKNTWNLSRNQSIALYR
                     LIQESLSNSSMHGKATEIRIFITFNTSNLIITISDNGIGCGNIKKGNGLNSINERIYE
                     LNGKVEFDNSNNGFTIRASFPKFSGGDFFE"
     gene            915..1562
                     /locus_tag="PC471_003066"
     CDS             915..1562
                     /locus_tag="PC471_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2790174.1"
                     /translation="MNKIKVLIVDDEKLIRKGLKIILSSYNDLEIVGDASNGYEALEF
                     CKMNDVDIVLMDIRMKVCDGVLGTRLIKEYNNSITLLILTTFNDDEYIKDAMKFGASG
                     YLLKDSSDEVLHEGIRSSFFGNIVLDKSVAEKIMTSEKTIKQEHLYDMYNLTEKEISI
                     IRLIANGLNNKEISQELFLSEGTIKNNITNILAKLELRDRTQLAIFAFKNKIVIE"
     gene            1691..2626
                     /locus_tag="PC471_003067"
     CDS             1691..2626
                     /locus_tag="PC471_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2790175.1"
                     /translation="MDIVRVNNITKRFNDKLVLDNISFSVKKGEIFGLIGPNGAGKST
                     LINIITNLMLPNSGSIQINGLDLSKDYIKAKSIIGLVPQELAIIETLTPFDNLEYFGA
                     FYGLKGKLLKERIIEALEVTGLTEVKKKKVKKLSGGMQRRLNIGIALLNHPKILILDE
                     PTVGVDPQSRNHIFNFIKDISKKHETTVIYTSHYMEEVEHLCSKIFIMDEGKEIAFGN
                     NDYLKSLVSTNTKLIMEIKNINAQLIFDLKNTKGVISVLENNSLLELDIDKKLQLTDI
                     LSVIDKNNSKIMKISYEEPSLEDVFLNLTGKNLRD"
     gene            2640..3737
                     /locus_tag="PC471_003068"
     CDS             2640..3737
                     /locus_tag="PC471_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2790176.1"
                     /translation="MKLYMSVKTMLKSLKSSFILNLIYFLALPLILSWFLGMVTESMF
                     QNPIKTESTPIVIYDKDNTRLSNDLTKYLKNDLSYILTVKKDDSKAELKLTIPKGYES
                     SLLNEKSNTLNIEKLGTRDDIAILLQDILDTYHEKFYLNNSQKISSEDFSKLFNKNSI
                     DTSIINNNVKQSSYEYFALVSLGFLVIIFIMNNILSNYISESKGLSKRLYSMPITRVQ
                     FLIYDFVGLWIYSFIFLLLYVLFFRILGITFKGNFAILLLLCALSSYFMTSISTFVTS
                     FFSKKYGTAIVYALLFLQTIFGGIFSMISDTFTKLTSLSPTYLIGELFSNYETFKTID
                     SIGNLIFTCLIISTILIALAIVKEKYKWREV"
     gene            3740..4804
                     /locus_tag="PC471_003069"
     CDS             3740..4804
                     /locus_tag="PC471_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2790177.1"
                     /translation="MIRLTIANIKRYLKNHTLLINMVMLPIILIFSLNFFINNSGNQS
                     MYFSPVAIVSDSSGKYEHKLINSSKLKENSFRLNEQDKAMNLLKNNKVSAVFVLDKNF
                     SNSIDKLQRPVVKCFKIENGGGSLWAESQIESFITKSLKLKVDKNIDDKLTKTNIIDN
                     KPDKNKGSFLVVFLICYFMYINAAHLASDLFTLKKSNVLKRLISTDNKDIKIIFSIFL
                     GLFFIQSIVYTFALLCFSIIEDFNLSLNVLMIVLANSFVSTGFVFWVARVFKNESSIS
                     LISTFYSLIGLAISISSLIPSMDKLSFMTNLSKFTPFYWTIDAIKNNGSILINIIALI
                     LIGIIFVTAGSLKLRDFAKN"
     gene            5048..5545
                     /locus_tag="PC471_003070"
     CDS             5048..5545
                     /locus_tag="PC471_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HDF2790178.1"
                     /translation="MYKIGESVMYPKEGACSVNDIVTKKINHEMQKYYELSVIFNSNL
                     KISIPVLNADRIGIRPVMDGNDVDNFIQSINKTDGVWIFDRKERLKLYQDKFHSGDVF
                     EIVKLIKMLMIQDSSKQLCSTDKEFLNKAQKFALSELAAAQCKSYTMVLEEMKKHILN
                     SKNTN"
     gene            complement(5721..5969)
                     /locus_tag="PC471_003071"
     CDS             complement(5721..5969)
                     /locus_tag="PC471_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2790179.1"
                     /translation="MSRKAYEEALVELEKFIDERKEIIKSAEDCIDKYIVDRTLPFDY
                     KDKCVEWQQELLDIAEAQVLEANELSVLLQEKKELEED"
     gene            complement(6035..7051)
                     /locus_tag="PC471_003072"
     CDS             complement(6035..7051)
                     /locus_tag="PC471_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HDF2790180.1"
                     /translation="MEYNELLKSARENLNGSCKVCKVCNGVACAGEVPGMGGKGSGSS
                     FIENSKSLEKVKVNMRVIHDVSNPDTSIEMFGKKMSAPIFAAPVTGTTLNMGGKINER
                     DYIEPVVAGCVNSGIYAMVGDTAVDAFLMENLDVVKKYNGAGIVFIKPWDNENIIKKI
                     RLAEEAGAFAVGVDIDACGLVTLSLHGKPVLPKNVKQIKELVKSTKLPFILKGIMTVE
                     DALMAVEAGVDAIVVSNHGGRVLDCTPGACEVLPKIADAVKGKVTILADGGVRTGLDV
                     LKMIGLGADAVLIGRPFVTASFGGATDGVETYVNKLQSELSSSMILTGCQTIKDIDGK
                     VIYK"
     gene            complement(7109..7876)
                     /locus_tag="PC471_003073"
     CDS             complement(7109..7876)
                     /locus_tag="PC471_003073"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HDF2790181.1"
                     /translation="MQDKSVREIKEIIETLEVEKYMEYIELLRVDERKSVQGLAIKLA
                     KKLDNIRKEEERLETINIFENEGYDKGYLYIGGIDEAGRGPLAGPVVASVVVFKKDTK
                     IEGVNDSKKLSEAKRDELFEVIKEEALDYGIGIVNNEEIDEFNILNATYMAMKKAINC
                     LKKAPDYLLVDAATIPGIDISQNPIVKGDSKSISIAAASILAKVTRDSIMYQYDRVYP
                     EYGFKSHKGYGTKEHYEAIEKYGITPIHRKSFLKNIL"
     gene            complement(7944..10133)
                     /locus_tag="PC471_003074"
     CDS             complement(7944..10133)
                     /locus_tag="PC471_003074"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin-dependent
                     ribonucleoside-diphosphate reductase"
                     /protein_id="HDF2790182.1"
                     /translation="MKYIIKRDGTKELFSKSKIEDAVYKASINSEGGVDRELAYGISR
                     KIANDYNSMEKCLSVEEIQDIVEVLLMESNRKDIAKHYILYRERRSEIRTKAWEFDEL
                     QKSIWKNKYRHNGESFDEWIKRISNNSSRISKLIRQKKFLFAGRILANRGLQKEGKKV
                     TYSNCYVIEPPKDSIEDIFDTAKKLARTFSYGGGVGIDISKLRPKGAKVNNAAKHTTG
                     AVSFMELYSMTTGLIGQRGRRGALMISMDVNHPEIEDFIDIKTDLEKVTKANISVRVT
                     DEFMQAVREKKLYNCTFEVETTGEKIVKIVNAYDLFNKFVKNNWDYAEPGILFWDKIK
                     TYNFMSEDKNFCFSGVNPCAEEPLPSGGSCLLGSINLSEFIIQPFGEHATFDINKLKS
                     CVREAVVALNDVLDEGLNLHPLQEQRDSVHKYRQIGLGVMGIADMLIKMGIRYGSDES
                     LELCDLIGKTMLNEAVKQSALIAKEYGTFSEYKYENISKSKFFIENLNEDTKEMVKKY
                     GLRNSQLLTIPPTGSISTMIGISGGIEPIFNLSYIRKTESLHDEDVYYKVYTPIVKEY
                     MDKKGITDEECLPDFFVTAMNIGYEERINMQKVWQKYIDASISSTINIPFETTVEDVF
                     NIYIKAWEAGLKGVTIFRDGCKRSGILINEKKEENNKEDKKENSKKEYYKNNKKEKDN
                     KDEKAKEIINKNFEGDEKFVCPECGSEGIAHTGGCSICLKCGYSGCN"
BASE COUNT         3562 a         1579 c         1190 g         3921 t
ORIGIN      
        1 taattcgatt tactttgttc ctttaatatt agatatatca tttttaataa aggaaaaata
       61 taagtacata ttgttctctt ttgttataat atcttctctt attattagtt tagataaaaa
      121 tatatatttg gcactggagt caagctctat attgctaatt ataactgtac tttctatata
      181 tatagaaaat gagaatttat caaaattata cagtcaaaat ctttatgata agcttagaat
      241 ttctaaagat gaattaaaaa aagttaatgc tgatttggaa acttatgcaa gttctataga
      301 agaattagca attttaaaag aaagaaatcg aatatcaaga gaaatacatg atagtgtagg
      361 acattctctt tctacaacaa taatacaatt aaatgctata gaaaaactct taaaagacaa
      421 acctctaatt tatgacttag tacatgaact tagagaattt gtaactgaaa gttttcaaga
      481 tgtaagaagg gctatatctg agttaaaacc tgttgaatat gaaaattacc aaagtttatt
      541 taaaatcaaa gaattagtta aaagctttat taagcttaca aatattaatg ttaaattaac
      601 tatttcaaaa aatacttgga acttatccag aaatcaatct atagctttat atagacttat
      661 acaggaatcc ctttccaact catcgatgca tggaaaggca actgaaatca gaatttttat
      721 tacattcaat acttcaaatc ttataattac tattagtgat aatggtatag gatgtggcaa
      781 tataaaaaaa ggaaatggac ttaatagtat caatgagaga atatatgaat taaatggtaa
      841 agttgaattt gataattcta ataatggttt cacaattaga gcttcttttc ctaaattttc
      901 agggggtgat ttttttgaat aaaattaaag ttcttatagt agatgacgaa aaattaatta
      961 gaaaaggtct taaaattata ttatcatctt ataatgattt agaaattgta ggagatgctt
     1021 ctaatggata tgaagcttta gaattttgta aaatgaatga tgtagacata gttcttatgg
     1081 atatacgaat gaaagtatgt gatggggttc taggtacaag gcttataaag gaatataata
     1141 attctataac attattaatt ttaacaactt ttaatgatga tgaatatata aaagatgcta
     1201 tgaagtttgg tgcttcagga taccttttaa aagatagctc tgatgaagta ttgcatgaag
     1261 ggattcgttc ttcatttttt ggcaatattg tgcttgataa aagtgtagct gaaaaaatca
     1321 tgacctctga aaaaacgatt aaacaagagc atctttatga catgtacaat ttaacagaaa
     1381 aagaaatatc tataattaga ctaattgcaa atggacttaa taataaagaa ataagtcaag
     1441 aactattctt atctgaaggt acaataaaga ataacataac taatatttta gctaaactag
     1501 aacttcgtga cagaactcaa ctagcaatct ttgctttcaa aaataaaatt gtaatagaat
     1561 aaatctaaaa atataaagtg acttaagtca ctttttttag tgacttatag taatagaatt
     1621 ataattcaca attctttatt ataatattat agctttttaa cttatacaaa attattttgg
     1681 agggattact atggatatag tcagagtaaa taatataaca aaaagattta atgataaatt
     1741 agtacttgat aacatcagct tttctgttaa aaaaggtgag atatttggac ttattggccc
     1801 aaatggtgca ggtaaatcta cacttataaa tataattaca aatcttatgt taccaaatag
     1861 tggtagtatt caaatcaatg gtttagattt atcaaaagat tatataaaag caaagtctat
     1921 aatagggtta gttccacaag aattagcaat tatagaaact cttacccctt ttgataactt
     1981 agagtacttt ggagctttct acggtttaaa gggaaaacta cttaaagaaa gaatcattga
     2041 agcattagaa gttacaggtc ttactgaagt caaaaagaaa aaggtcaaaa aattatctgg
     2101 tggtatgcaa aggagattaa atattggaat agcactctta aatcatccta aaatattaat
     2161 attggatgaa ccaactgtag gtgtagaccc ccaatcaaga aatcatattt tcaattttat
     2221 aaaagatata tctaaaaaac atgaaactac tgtaatatat acttcccatt atatggaaga
     2281 agttgagcac ctttgttcta aaatttttat tatggatgaa ggtaaagaaa ttgcttttgg
     2341 aaataatgat tatttaaaat cactcgtatc tacaaataca aaattaatta tggaaataaa
     2401 aaatataaat gcccaactta tctttgattt aaaaaatact aaaggtgtta tatctgtttt
     2461 agaaaataat tcattattag aattggatat agataaaaaa ttacaactta cagatatact
     2521 ctctgtcatt gacaaaaaca attcaaaaat aatgaaaatt tcttatgaag aacctagcct
     2581 agaagacgta tttttaaatc taacaggaaa aaatttaagg gactaatgga ggtgatttta
     2641 tgaaattata catgagtgta aaaactatgc taaagagttt aaaaagctcc tttatactta
     2701 atttaatata ttttttagct ttgcctctga ttttatcttg gtttcttggg atggttacag
     2761 aaagcatgtt tcaaaaccct ataaaaacag aatctacccc cattgttata tatgacaaag
     2821 ataacacaag attatcaaat gatttgacaa agtatttgaa aaatgattta tcttatatat
     2881 taacagttaa aaaagatgat tctaaggcag aattaaagct tactatacct aaaggctatg
     2941 agagtagttt attaaatgaa aaatcaaata ctctaaacat agaaaaactt ggaactcgtg
     3001 atgatattgc aatattgctt caagatatat tagatactta ccatgaaaaa ttttatttaa
     3061 ataattctca gaaaataagt tcagaagatt tttctaaatt atttaataaa aattctattg
     3121 atacttctat aataaataac aatgttaaac agagctctta tgaatacttt gctttagttt
     3181 cactaggatt tcttgtaata atttttataa tgaacaatat tttgtcaaat tatataagtg
     3241 aatcaaaggg actttcaaaa agactttatt ctatgcctat aactagagta caattcttaa
     3301 tttatgactt tgtaggttta tggatttatt catttatatt cttattatta tatgttttat
     3361 tctttagaat acttggaata acatttaaag gtaattttgc catattgctc ctactttgtg
     3421 ctttatcatc ttattttatg acttcaatat ctacctttgt aaccagcttt tttagtaaaa
     3481 aatacggtac agcaatagtt tatgcattac tctttttaca gacaattttt ggaggtatct
     3541 tcagtatgat aagtgataca tttactaagc ttacaagttt atctcctacc tatttaatag
     3601 gagaattgtt tagtaattat gaaacattta aaaccataga ttcaatagga aatttaattt
     3661 tcacatgttt aattatctct acaatattaa ttgcacttgc tattgtaaag gaaaaatata
     3721 aatggaggga ggtttaataa tgattagatt aactattgca aatataaaaa gatatctaaa
     3781 aaatcatact cttttaatta atatggttat gcttcctatt atactaattt tttcacttaa
     3841 tttttttatc aacaactcgg gaaatcaatc aatgtacttt tctccagtag caattgtatc
     3901 tgactcttca ggaaagtatg aacataagtt aataaatagt tctaaattaa aagaaaattc
     3961 atttagatta aatgaacaag ataaggctat gaatctatta aaaaataata aggtttctgc
     4021 tgtattcgtt ttagataaaa atttttcaaa tagtatagat aaattacaac gacctgtagt
     4081 taaatgcttt aaaattgaaa atggtggtgg tagtctttgg gcagaatcac aaatagaatc
     4141 ttttattact aaatcgttaa aattgaaagt ggataaaaat atagatgata aattgacaaa
     4201 gacaaatata attgataaca aaccagataa gaataaaggt tcatttttag tagtcttctt
     4261 gatatgctat tttatgtata ttaatgcggc acatcttgct tctgatttat ttacattaaa
     4321 aaaatcaaat gtgctaaaaa gacttatctc tacagataat aaagatataa aaataatttt
     4381 cagtatattt ttaggactat tttttattca atcaattgta tacacttttg ctctattatg
     4441 tttttcaatt atagaagact ttaatttgag tctgaatgta ttgatgattg tactagcaaa
     4501 tagttttgtt tccactggat ttgtattttg ggtagctaga gtatttaaaa atgaaagttc
     4561 tataagtctt atatctacat tttattcttt aataggtcta gctatatcta tctctagtct
     4621 aataccatca atggataaat tatcctttat gactaatctt tcaaagttca caccatttta
     4681 ttggactata gatgctatta aaaacaatgg tagtatttta ataaatataa tagctctaat
     4741 attaattgga attatatttg taactgcagg aagtcttaaa ctaagagatt ttgctaaaaa
     4801 ctaataatta ttaaatataa ttagtatcta tttttatata attgtaaaag aatatttcaa
     4861 catattaaat tattttatct tatatttttc tctttagttt cacacatcac caagtctaaa
     4921 ctctatatta tatattaata tttttgactt gtcaagttat ttatgttata atattaatca
     4981 ttagataatt aaagcattgt acctaagttg caatatctaa aattcaattt ggaggtataa
     5041 ctagcatatg tataaaattg gtgaaagtgt aatgtatcca aaagagggag cctgctctgt
     5101 aaatgatatt gtaacaaaga aaataaatca tgagatgcaa aaatactatg aattaagtgt
     5161 catttttaat agtaacctta aaatttcgat tccagttcta aatgcagata gaattggcat
     5221 acgtccagtt atggatggaa atgatgttga taattttatc caatctatta ataaaacaga
     5281 tggtgtatgg atttttgata gaaaagaaag acttaaactg taccaagata aatttcattc
     5341 aggagatgtt tttgaaattg taaaattaat caaaatgctt atgatacaag acagttcaaa
     5401 acaattgtgt agcacagata aggagttttt aaataaagct caaaaatttg ctttatcaga
     5461 attagctgct gctcaatgta aatcctatac aatggtatta gaggaaatga aaaaacatat
     5521 attaaattca aagaatacaa attaaaccag ttttttaaac tggttttatt ttttatacta
     5581 atcatccatg ctactttagc ttattttaaa ctgcctaatc taatattaat aaagttatct
     5641 caattagata gatgtatttt attcaaataa aaaaaactaa gagtaaatca tattactgat
     5701 ttactcttag ctaataaaat ttaatcttct tcaagttctt ttttctcttg taataataca
     5761 cttaattcat ttgcttctaa aacttgtgcc tctgcaatat caagtaactc ttgttgccat
     5821 tccacacatt tatctttgta atcaaaaggt agcgttctgt caactatgta cttatcaata
     5881 cagtcctccg cacttttaat tatttctttt ctttcatcaa taaatttttc taattctact
     5941 aaggcttctt cataagcttt tctactcata taaattcccc ctttgtacaa ttattctaca
     6001 ttaaaatgat ttttcctctt ttttgtataa aattttattt gtatataact tttccatcaa
     6061 tatcttttat agtttgacat ccagttaata tcatagaact acttagctct gattgaagtt
     6121 tatttacata agtttctaca ccatcagttg caccaccaaa agaagcagtc acaaaaggtc
     6181 ttcctattaa tactgcatct gcacctaaac ctatcatttt tagtacgtct aaaccagttc
     6241 tcactccacc atctgcaagt attgtaactt tacctttaac agcatcagca atttttggta
     6301 aaacttcaca tgctcctggt gtacagtcaa gcactcttcc tccatgattt gatacgacta
     6361 ttgcatccac acctgcttct actgccatta aagcatcttc aacagtcatt atacctttca
     6421 atataaatgg tagtttagta gactttacta actcctttat ttgtttaaca tttttaggaa
     6481 gtactggttt tccatgtaat gataaagtta ctagaccaca ggcatctata tcaactccaa
     6541 ctgcaaatgc accagcttct tcagctaatc ttatcttttt tataatattt tcattatccc
     6601 aaggtttaat aaatacaatt ccagctccat tatacttttt gactacatct aaattttcca
     6661 ttaaaaaagc atctactgcc gtatcaccaa ccattgcata aataccacta ttaacacatc
     6721 ctgcaactac tggttctatg taatctcttt cattaatttt tccacccata tttaaagtag
     6781 ttcctgttac tggagctgca aatataggtg cactcatttt ctttccaaac atctctatag
     6841 atgtatcagg atttgataca tcatgtatta ctctcatatt tacttttact ttttctaaac
     6901 tcttgctatt ttctataaaa gaagaaccac ttcctttacc tcccatgcca ggcacttcac
     6961 ctgcacaagc aaccccatta caaaccttac aaactttaca acttccattt aaattttctc
     7021 tagcactttt taataattca ttatattcca tctttaatcc cccattaact atagtaattt
     7081 atattatata cattatatca tattatattt acagtatatt tttcaaaaaa ctttttctgt
     7141 ggataggagt tatgccatat ttttcaatag cttcatagtg ttctttagtt ccatatcctt
     7201 tgtgagattt aaacccatat tctgggtata ctctatcata ttggtacatt atactatccc
     7261 tagtaacttt agcaagtata cttgctgctg ctattgatat agattttgaa tctcctttca
     7321 caattggatt ttgagatatg tcaatacctg gtattgttgc tgcatcaact aataaataat
     7381 ccggtgcttt ttttaaacaa tttatagcct ttttcattgc catatatgta gcatttaata
     7441 tattaaattc atctatttct tcattgttta ctatacctat tccataatct agtgcttctt
     7501 cttttattac ttcaaaaagt tcatctcttt ttgcctcact cagtttctta gagtcattta
     7561 caccttctat tttggtatct tttttaaata ctacaacaga agctacaaca ggtccagcaa
     7621 gtggtcctct tcccgcctca tcaattcctc caatatataa ataaccttta tcatagcctt
     7681 cgttctcaaa tatatttata gtttccaatc tttcttcttc ttttctaata ttatccagct
     7741 tttttgccag ttttatagct agaccttgta cagattttct ttcatcaact cttaacagct
     7801 ctatatattc catatatttt tctacttcta aagtctctat tatttcttta atctccctca
     7861 cacttttatc ctgcatacca ttccctcatc tttcgttttt aaaaatttaa tttttaaata
     7921 tattaaaatt ttttaattag agtttaattg catccactat agccacattt taaacatata
     7981 ctacatccac ctgtatgtgc aattccttca cttccacact cagggcacac aaatttttca
     8041 tctccttcaa aatttttatt tattatttct ttggcttttt catctttatt atctttttct
     8101 tttttattat ttttataata ttctttttta ctattttctt ttttatcttc tttattattt
     8161 tcttcctttt tttcatttat taaaattcca ctccttttac atccatctct aaatattgtt
     8221 actcctttaa gaccagcctc ccatgcctta atatatatat tgaaaacatc ttcaactgta
     8281 gtttcaaaag gtatatttat tgtagatgat atagaagcat caatatactt ttgccatact
     8341 ttttgcatgt ttattctttc ttcatatcct atattcatag cagtaacaaa aaaatctggt
     8401 aaacattctt catctgtaat acctttcttg tccatatact ctttaacaat tggagtatat
     8461 actttataat acacatcctc atcatgcaag ctttcagttt ttcttatata tgaaagatta
     8521 aatataggtt ctattccacc actgatacct atcattgtag atatggaacc tgtaggaggt
     8581 atggttaaaa gttgtgaatt tctaagccca tactttttaa ccatttcctt agtgtcttca
     8641 tttagatttt ctataaaaaa cttagattta gaaatatttt catatttata ctcactaaat
     8701 gttccatatt ctttagcaat taatgctgat tgcttcacag cttcatttaa catagtctta
     8761 ccaattaaat cacatagttc taaagattca tcagaaccat atcttattcc catttttata
     8821 agcatatcag ctatacccat tacacctaaa cctatctgtc tatacttatg tacactatct
     8881 ctttgttctt gtaatggatg caagttcaat ccctcatcta agacatcatt taaagctact
     8941 acagcttctc taacgcaaga ttttaattta ttaatatcaa aagtagcatg ttcaccaaat
     9001 ggttgtatta taaactctga caagtttatt gagccaagta gacaacttcc tccacttgga
     9061 agtggttctt ctgcacaagg atttactcct gaaaagcaaa agttcttatc ttcactcata
     9121 aaattatatg tttttatttt atcccaaaac aatatacctg gttctgcata atcccagtta
     9181 tttttcacaa atttattaaa taaatcataa gcatttacta tcttaactat tttttctcca
     9241 gtagtttcaa cttcaaaagt acaattatat aacttttttt ctcttacagc ttgcataaat
     9301 tcatctgtta ctcttacaga aatatttgct ttagtaacct tctccaaatc tgtttttata
     9361 tctataaagt cttctatctc tggatgattt acatccatag aaatcataag agctcctctt
     9421 ctccctcttt gaccaattaa gccagttgtc atactgtaaa gttccataaa agagactgct
     9481 ccagtcgtat gttttgcagc attgttaact ttcgctcctt ttggtcttag ctttgaaata
     9541 tcaattccaa ctcctccacc ataactaaaa gttctagcaa gtttttttgc agtatcaaat
     9601 atatcttcta tgctatcttt tggtggttct attacataac aattagaata tgttactttt
     9661 tttccctctt tctgtaatcc tctatttgct aaaattcttc cagcaaatag aaattttttt
     9721 tgtcttatta gttttgatat tcttgaacta ttatttgaaa ttctctttat ccattcatca
     9781 aaactctcac cattatgtct atacttgttc ttccatattg atttttgtaa ttcatcaaac
     9841 tcccaagctt tagtccttat ttcagagcgt ctttctctat ataatatgta gtgttttgct
     9901 atatcttttc tatttgattc cataagtagt acttcaacta tatcttgtat ttcttctacg
     9961 gataagcatt tttccataga attatagtca ttagctattt ttcttgatat accatatgcc
    10021 aactctctat ctactccacc ttcactattt atactagctt tatagacagc atcttcaatc
    10081 ttacttttac taaaaagttc ttttgtacca tctcttttta ttatgtattt catttttaaa
    10141 cctccaataa attagtatta aattaataca cacattaact ataactctaa acaaactttt
    10201 tatatatttg tatattttca tattaattat ctaacataat atatattata ct
//
LOCUS       DAMJYR010000126        10090 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.131, whole genome shotgun sequence.
ACCESSION   DAMJYR010000126 DAMJYR010000000
VERSION     DAMJYR010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10090)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10090)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10090
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.131"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(100..1980)
                     /locus_tag="PC471_003075"
     CDS             complement(100..1980)
                     /locus_tag="PC471_003075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp10"
                     /protein_id="HDF2790183.1"
                     /translation="MSKRKSFIRTIAVSTMAVAVTGSATCAYAAPVLQGTKTYEKVNT
                     IDISVDSVENIVYSFQASIKVQGEVEVVDNEQKEKITWSDNIKSQIKSGNANATCRAE
                     YNKSSNTTTLDIYVTSNEDLLDGNRLNIGRISVKKSGSNSNADYKVLGKGTNDKPALK
                     IVTYNNKTVDYENISSDEGLIFTLINESEVKPIGGTGSSKNDPEKYKVEKSEALEYLL
                     NNIRINYSIVSKETQESGSNVILKLGLAQKTTKGRKVTINKYVEVTLPKSLEYIVENE
                     LSKPDELPPDNGSGGNNGGGSNSGGSSSGGSSGGGNSSDSTSNVTVKKLKGDDRFETA
                     IKISQSGWTKSDTVVIVNGEDKSMVDGLTATPLASVKNSPILLSSNDKLPQKTVEELK
                     RLNPSKVVVIGGNNSMPNSVVEAIKAVNSKISVQRIGGDTRYQTSINIAKEIDKTNNV
                     SKLYIGAGNGEADSLSIASLAGKEKTPIVLTQKDGVDNEAEQFIKSNKVSNIYFIGGV
                     EKISNKAIEQVGKIANKDISNNRVAGQTRQETNAKVIDKFYSQSKLDGVVVANQDKLI
                     DALAVGPLAAKNNSPVILATNTLDKSQESSLKGKNSSKLFEVGGGIASSVIDKIKSLI
                     EK"
     gene            complement(2190..8153)
                     /locus_tag="PC471_003076"
     CDS             complement(2190..8153)
                     /locus_tag="PC471_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding adhesion protein"
                     /protein_id="HDF2790184.1"
                     /translation="MKKAISCVLAVSMCSAPLNVFAEPILEGKLRAVEKSVTEKLRGS
                     LEVDLNFSLPIKNSDSESMTNMQLRLKDDSNNSGIIKLGEKDSGTVNVGTDNIEYTIK
                     KLSASRSEIKNKDENISYYNIVFNNLPVGKYNVEVSGDGFKNKEIKDIDISSYSQRVL
                     LSNRASVDNKNKPLNYELFLMGDVNGDGKVDKSDYNKVLENIDSNKREFDLNRDGKVD
                     IVDLDYVQKNLGQSEDAKSLESIVSTNPIVDTSKVELKGNSDVDINGNVENLFSGENN
                     GVTISSKNDEISEEKPAVMDIALSEALNMEVINIKAPEGTAPAKGIVEVTDKDGNVQQ
                     IPYENKEYMKNLKDEEQQEEVNNEQEEVNQEEVNNKDENINQTDNNSNNKDENNDEQQ
                     ENSDKQEVNSNTNQDKNNDKENSSMDKPNSDEDTPKEEGNISKDDNSTTSTYSKSTLE
                     TYADVQNSDIVINLGKQVAVKKITIKITATTANDRNLAEISKVEFLNNVYKEIPKPEM
                     NIPKINKISTSTAVGNEKVSFYWNNEVNVSAYELILDKIDSKGKVLSTKKLQTSKNTI
                     EVRDIEAYGLYRVSIQSLNGDWSSGYKDATPENLDGVPENVDSNYKPIQFSPDSILEF
                     QVVPDTKPEPPEGINVKGKFKSLDVSWKNHKQAKDFDLYYKEKGSNGSWIKYNDKLIR
                     GTSSKIEGLKDNTTYEVRMTATNHLGTSGMSKTYIGTTESMDAPTTPNYRLINTPKEN
                     GGFGIPTNHIVDVTYPAGYDKSEYSDKNPFDKFNVVDGDFTTHWTFPTYNAAEGTNRG
                     PIITFDKSYTMDTFMATPRFDVSDKTINDFEVRYWDENGQLHNLGDVPFETKTDPVNN
                     NKYVMVRLKEPITATKIQVSVCSSYSYKSNISELKFYEYDSIEDDVRNLFMDDLQVEL
                     KEDVTQEKITELKDRLNTPDKASGEYHPYKTVIERELKLAQDLYNDRATLTDVTTVNQ
                     EINKLGKYKDKNGQYTVNSNNLGMQNDWQALGVAARAGDEITVYVGSKSGKTPKLIYT
                     QYYGESGAYKSGEINLNTGKNVIQLSKLHSLDIECGGSLYIRYAEDTPSGGDIKVRVA
                     GATKIPHLNLNGTINDKSPAGVSESKKKIKAYIEELARYKDAVKGEPYYPGTNVGNSY
                     GYSEKTGVLNTTDIESDKVTLNVPATAVYQGISKGNADLNTQVDRLYNSMLAWEQIMD
                     LVYSERGVFKTQDLDGNGTVDDNEFNLTKNDMAPKSRMNIKYQRMFIGAFMYASGLHV
                     GVEYGSVPGLMNGVPFQIDDSGKATGGNLFGWGIGHEIGHVTDIGKMTYSETSNNVLA
                     LLAQTFDDKTHSRLEGSTMDKIYEHVTSNSLGIPSNVFERLGMLWQLHLAYDDDFTGS
                     MLKNNSDADLSNDTFYAKISRKYRALSSSDPINSLPKDQMLVAMASSVVEKDLRDYFK
                     AWGVEITPELNSIMDSKNYEKESRKIQYVNDEARRKILNENIASMAKDTKVSASFSDG
                     IQSGSLVKSNKISIDLDVDKDKDKILGYEIIRSDGNYLDGENGTQVKYRTVGFVDAKG
                     NSKTKFTDNISPLNNRAFTYKVVAYDYHLNPTEEFEVGTVKLSDEGKINKSAWSFITN
                     TVSDGDVRTENDSHGPLQNPEIDNIKDNDANTTYKGKIISKAEWNKDPQKDPDINVEE
                     NPYIIVDMKETLPIVGLKYTKPETEARKFSLKGLFNFRKNTSTTYNPLTNYKIQVSND
                     KKNWEDVSQGTFEYGQDLVGGGKDQDNEARVTFNKDGKLWTYQARYVKLISNNKSNIE
                     VAELDIIGPPGDNIEIGTMNNADETRTNGIGKLAEDYVYQSDDSSTSEDETGKIPAGS
                     IVITGEYSGNPAFNLPLLIDKNNKTISGEALLFAEVPEKGELGEISKGTWVYYMPKEN
                     FDSLSDKVKAELYRYNDLQGDTPVGQRFVSDTMYVPVGAKNYDELKTISLTDSNSKAR
                     KATRNSIDISNKKVITVSNKVKGNIVRDK"
     regulatory      complement(8277..8362)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            complement(8534..9937)
                     /locus_tag="PC471_003077"
     CDS             complement(8534..9937)
                     /locus_tag="PC471_003077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp9"
                     /protein_id="HDF2790185.1"
                     /translation="MKNSKKILAIGLTLFLVMVNTPMVNALTSVEQIKGNDRYETAAK
                     IADKQNYNTAILINSDNSLADGLSASGLAGALNAPILMTKQNQIPNTTMERLNKAKTV
                     YIIGSESTISKNVENQLLSKKKVVQRIFGENRFDTSIKIAEKIKEIKPIDKVIIANGF
                     TGEADAISASPVAARDGVPIILTDGNSVGFDTTGLKSYALGSSEIISDELVKSTNSIR
                     LGGTDRFETNKIVIQEFYKNSKEFYLSKGLQLTDALAASTIAKNAPVVLVENGSNKSI
                     LSGADKLTVLGGINQNVIKQCINQASPNQQGLYYNPNDRAFKERIKGKVYALTKQYRK
                     ENGVRALSVASRLEGLANDWSNLMANKKTLSHTINGKNSYSTFLKYLDWSEIKPGYIA
                     VQGENIIKYKIPDKPVYTNRDADDIGNFIFNEWKTNPEEGTNMLHKGYEIMGFGIAIT
                     GDKNLYATHEFYGRYKE"
BASE COUNT         2761 a         1881 c         1153 g         4295 t
ORIGIN      
        1 atattattag attgttaaga taatgaaaat aaaataagaa tgcatttaaa tatataactt
       61 tatatctaaa tacattctta ttttctattt acttaatatt tatttttcaa ttaaactctt
      121 aatcttgtct attacagatg aggctattcc tccacctact tcaaacaact ttgatgaatt
      181 tttacctttt aatgacgatt cttgtgattt atctaacgta tttgtagcaa gtataactgg
      241 tgaattgttt ttagctgcta atggtccaac tgctaaagca tctataagtt tatcttgatt
      301 tgcaactaca actccatcta gcttagattg agagtagaat ttatctatta ctttggcatt
      361 cgtctcttgt ctagtttgac ctgccaccct attgtttgat atatctttgt ttgctatttt
      421 tccaacttgt tcaatagcct tgttagatat tttttctact ccaccaataa aatatatatt
      481 tgacacctta tttgatttaa taaattgttc agcttcatta tctacaccat ctttttgtgt
      541 aagtactatt ggagtttttt ctttaccagc cagtgatgct attgataatg aatcagcttc
      601 tccatttcct gctcctatat acaacttgct tacattattc gtcttatcta tttcttttgc
      661 tatattaata gaagtttgat atcttgtatc tccacctatt ctttgtactg atatttttga
      721 atttacagct ttgatagctt caacaactga atttggcatt gagttgtttc cacctatcac
      781 aactactttt gatggattta gccttttcaa ttcttctaca gttttttgag gaagtttatc
      841 atttgatgat aaaagaatcg gtgagttttt tacactagca agtggtgtag ctgtaagccc
      901 atccaccata cttttatctt ctccatttac tattacaact gtatctgatt ttgtccaacc
      961 actttgagat attttgatag ctgtctcaaa tctatcatca ccttttaatt ttttgactgt
     1021 gacattagat gtagaatcac ttgaatttcc tcctccagag cttccaccac tagatgagcc
     1081 gcctgaatta cttcctccac cattatttcc accactacca ttatctggtg gtaattcatc
     1141 cggttttgat aattcatttt caactatgta ttctaaactt tttggaagtg taacttcaac
     1201 atatttgttt attgtaactt ttcttccctt agttgttttt tgtgctaatc caagttttaa
     1261 tattacattt gaaccacttt cttgtgtttc tttactaaca atagaatagt ttatcctaat
     1321 attgtttaat aaatattcaa gtgcttcact tttctctacc ttatactttt cagggtcatt
     1381 tttactagaa ccagttcccc caatcggttt tacttcactc tcattaataa gggtaaatat
     1441 taatccttca tcagagctta tattttcata atccactgtt ttgttgttat atgttacaat
     1501 ttttaaagct ggtttatcat ttgttccttt tcctaaaact ttatagtcag catttgagtt
     1561 acttccagat ttttttacag atattcttcc tatattcaat ctatttccgt ctagtaagtc
     1621 ttcatttgat gtaacatata tatctaatgt agttgtatta cttgatttgt tgtattctgc
     1681 tctacaagta gcatttgcat ttccactttt tatctgagat tttatattat cagaccaagt
     1741 aatcttttct ttctgctcat tatctactac ttcaacctca ccttgtactt ttatactggc
     1801 ttgaaatgaa tatactatgt tttctactga gtccacagat atatctatag tatttacttt
     1861 ctcatacgtt tttgttcctt gtaaaactgg agctgcatat gcacatgtag cactaccagt
     1921 tacagctact gccatagttg atactgctat agttcttata aaagattttc ttttactcat
     1981 taacacccgt ccttatctat cattaactaa gtggataaat ttacttatcc acctagcttt
     2041 tatatttaat ttataaatta ccctaaattt atgattacat ttatatactt tacttttaat
     2101 atctactatt ttaaaactat tctagtacct aatcttatta gatatacaat gttagtgtta
     2161 gtatttttac gtttttagag ttccacctac tatttatctc taacaatatt accctttact
     2221 ttattgctta cagttataac cttcttatta ctgatatcaa tagaatttct tgtagctttt
     2281 ctagctttac tattactatc agtaagactt attgtcttta actcatcata attcttagct
     2341 cctacaggta catacattgt atcacttaca aatctttgac ctactggagt atctccttga
     2401 aggtcattgt atctatatag ttcagcctta accttatcac ttaaagaatc aaagttttct
     2461 tttggcatat agtaaaccca tgtaccttta ctgatttcac ctagttcacc tttttctgga
     2521 acttctgcaa ataaaagtgc ttctccagat atagtcttat tgtttttatc tataagtagt
     2581 ggtaaattaa atgctggatt tccactgtat tcacctgtaa taactattga acccgctgga
     2641 atcttacctg tttcatcttc acttgtgcta gagtcatcac tttgatatac ataatcttct
     2701 gctaattttc caattccatt agtacgagtt tcgtctgcat tattcattgt gccaatttct
     2761 atattatcac ctggaggtcc tattatatct aattctgcaa cttctatatt agacttatta
     2821 tttgagatta gttttacata tctagcttgg tatgtccaaa gtttaccatc tttattaaat
     2881 gtaactctag cttcattatc ttggtctttt cctccaccaa ctaaatcttg tccatattca
     2941 aaagttcctt gacttacatc ttcccagttc tttttatcat tacttacttg tattttgtaa
     3001 ttagttaaag gattgtatgt tgtgctagta ttctttctaa agttaaataa tccttttaat
     3061 gagaacttcc tagcttctgt ctctggtttt gtatatttta gtcctactat aggtaatgtt
     3121 tctttcatgt ctactattat atatggattt tcctctacat ttatatcagg gtctttttga
     3181 gggtctttgt tccactctgc tttacttatt atctttcctt tatatgtagt atttgcatca
     3241 ttatctttta tattatcaat ttcaggattt tgtaatggtc catgtgagtc attttcagtt
     3301 ctaacatcac cgtcagaaac agtatttgtt atgaatgacc atgctgattt atttattttc
     3361 ccttcatctg ataattttac agtaccaact tcaaattctt ctgttggatt taggtgataa
     3421 tcatatgcaa ctaccttata tgtaaatgct ctgttattaa gaggagatat attatcagta
     3481 aatttagtct tactatttcc ttttgcatcc acaaatccaa ctgttctgta ttttacctgt
     3541 gttccatttt caccatctaa ataatttcca tcacttctta ttatttcgta tcctagtatc
     3601 ttatctttat ccttatctac atctaggtct atagatattt tattagattt aactagagaa
     3661 ccactttgta ttccatcaga aaaactagca cttactttag tatcttttgc catactagct
     3721 atattttcat ttaaaatctt tcttctagct tcgtcattta catattgtat ctttcttgat
     3781 tctttttcgt agttcttact atccattata ctgtttaatt ctggtgtaat ttctactccc
     3841 catgccttaa agtaatctct taaatctttt tcaacaacac ttgatgccat tgctactagc
     3901 atttggtctt ttggcaaact attaattggg tcactacttg aaagtgctct gtattttctt
     3961 gaaatctttg catagaatgt atcattactt aaatcagcat ctgagttatt ctttaacatt
     4021 gaacccgtaa aatcatcatc ataagctaga tgtaattgcc ataacattcc aagcctttca
     4081 aatacattgc ttggtatacc taagctattt gaagttacat gctcatatat cttatccatt
     4141 gttgaacctt caagtcttga atgtgtctta tcatcaaagg tctgagcaag taaagcaagt
     4201 acattgttac tagtttcgct atatgtcatc tttccaatat ctgtaacatg tcctatctca
     4261 tgacctattc cccatccaaa taagtttcct cctgtagctt ttccactatc atctatttgg
     4321 aaaggtactc cattcataag ccctggaact gaaccatatt ctactcctac atgtaatcca
     4381 gaagcataca taaatgctcc aataaacatt ctttggtatt ttatgttcat acgagatttt
     4441 ggagccatat catttttagt taagttaaat tcattatcat ctacagttcc attaccatct
     4501 aaatcttgtg ttttaaatac tcctctttct gagtaaacta aatccattat ttgttcccat
     4561 gcaagcatac tattatataa tctatctact tgtgtattta aatcagcatt tccttttgat
     4621 atcccttgat atacagctgt tgcaggaaca tttaatgtta ctttatcact ttcaatatct
     4681 gtagtgttta acacaccagt tttttctgaa tatccatatg aattaccaac atttgttcct
     4741 ggataatatg gttctccttt tacagcatct ttatatctag ctaattcttc tatatatgct
     4801 tttatctttt tcttagattc agaaactccc gcaggactct tatcatttat tgttccattt
     4861 agattaaggt gtggtatctt agtagctcct gctacacgta cttttatatc tcctccactt
     4921 ggagtatctt cagcgtatct tatgtataaa cttcctccac attctatatc taaactatga
     4981 agtttgctta attgaattac attttttcca gtgttaagat ttatttctcc actcttatat
     5041 gcaccagatt ctccatagta ctgtgtatat atcaacttag gagtttttcc acttttactt
     5101 ccaacatata ctgtgatttc atctcctgct ctagcagcta ctcctaatgc ctgccagtca
     5161 ttttgcatac ctaaattatt ggaatttaca gtatactgtc cgtttttatc tttatatttt
     5221 cctaatttat ttatttcttg atttacagta gttacatcag taagtgtggc tctatcatta
     5281 tataagtctt gagctaattt taattctctc tcaataactg tcttatatgg atgatattct
     5341 ccactagctt tatctggagt atttaatcta tcttttaatt cagttatttt ttcttgagtt
     5401 acatcttctt ttaattcaac ttgtaggtca tccataaata gatttcttac atcatcttct
     5461 atactgtcat actcataaaa cttaagttct gatatattag acttatatga ataggatgaa
     5521 caaacactta cttgtatctt ggtagcagtt attggttctt ttaatctaac cattacatac
     5581 ttattattat ttacagggtc tgtctttgtt tcaaaaggaa catcccccag attgtgtaat
     5641 tgaccatttt catcccaata tcgtacctca aagtcattta ttgtcttatc agaaacatca
     5701 aaacgaggtg tagccataaa agtatccata gtataagatt tatcaaaagt tattattggt
     5761 cctctattcg ttccctcagc tgcattatat gttgggaatg tccaatgtgt tgtaaagtcc
     5821 ccatctacta cattgaattt atcaaatgga tttttatcac tatattcact tttgtcatat
     5881 cctgctggat aagtaacatc tacaatgtga tttgttggta ttccaaatcc accattctct
     5941 ttaggagtat taattaatct atagttaggt gttgttggtg catccatgct ttcagttgta
     6001 cctatatatg ttttagacat tccactagtt cccaagtggt tagtagctgt cattctaact
     6061 tcatatgttg tattatcctt aagaccttct atctttgaac ttgtcccacg aataagctta
     6121 tcattgtatt ttatccaaga cccattagag cctttttctt tatagtacaa gtcaaaatct
     6181 ttagcttgtt tatgattttt ccagcttaca tcaagagatt taaatttacc tttaacattt
     6241 ataccttctg gtggttctgg ttttgtgtct ggaacaactt gaaattctaa tatactatct
     6301 gggctaaatt gtataggttt atagtttgaa tctacatttt ctggtactcc gtctaagttt
     6361 tctggtgttg catctttata accactagac caatctccat ttaaagattg tatgctaacc
     6421 ctatatagtc catatgcttc tatatctcta acttctattg tatttttact ggtttgtaat
     6481 tttttagtag ataatacttt tcccttacta tcaattttat ctagtataag ttcgtatgct
     6541 gatacattta cttcattatt ccaataaaaa gatacttttt catttccaac agcagtgcta
     6601 gtcgatattt tatttatttt aggtatattc atctctggct ttggtatttc tttatataca
     6661 ttgtttaaaa attcaacttt tgatatttct gcaagatttc tatcgtttgc tgttgtagct
     6721 gttattttta tagttatctt ttttactgct acttgtttac caagatttat tactatatca
     6781 ctattttgaa catctgcata tgtttctaat gttgacttac tatatgtaga tgttgtagag
     6841 ttatcatctt tactgatatt accctcttct tttggagtat cttcatctga attaggttta
     6901 tccattgatg aattttcttt atcattattt ttatcttgat tggtattaga gtttacttct
     6961 tgtttatcgc tattttcctg ttgttcatca ttattttcat ctttattatt tgagttatta
     7021 tcagtctggt ttatattttc atctttatta tttacttctt cttgatttac ttcttcttgt
     7081 tcgttattta cttcctcttg ttgctcctca tcttttaagt ttttcatata ttctttgttt
     7141 tcataaggaa tctgttgaac attaccatct ttgtcagtaa cctcaactat tccttttgct
     7201 ggagcagtac cttctggagc ttttatattg atgacttcca tattaagtgc ttctgataaa
     7261 gctatatcca ttactgctgg tttttcctca gatatttcat cattttttga agatattgta
     7321 actccattat tttctccact aaataaattt tctacattac cattaatatc tacatcacta
     7381 tttcctttta attcaacctt acttgtatct actataggat tggtagatac tatactttct
     7441 aggctttttg catcttcact ttgtcctaaa tttttctgta cataatctaa gtcaacaata
     7501 tctacctttc catctctatt taaatcaaat tctcttttat ttgaatctat attctctaaa
     7561 accttattat aatcactctt atctacttta ccgtctccat ttacatcacc cattaagaac
     7621 aattcataat ttaaaggttt atttttatta tctacacttg ctctattgct taataagact
     7681 ctttgtgaat aacttgatat atcaatgtct ttaatttctt tgttcttaaa tccatctcca
     7741 gagacttcta cattgtattt tccaacaggt aaattattaa atacaatatt ataatatgat
     7801 atattctcat ctttattttt tatttcactt ctacttgcag ataatttttt tatagtatat
     7861 tctatattat ctgtcccaac atttactgtt cctgaatctt tttctccaag ctttattatt
     7921 ccactattgt tactatcatc ttttaatcgt aactgcatat tagtcatact ttctgaatcg
     7981 ctatttttta ttggcagtga aaaatttaaa tctacttcaa gactccccct taacttttct
     8041 gtaactgatt tttcaactgc ccttagcttc ccctctaata taggttctgc aaaaacgttt
     8101 agtggtgcac tacacataga tactgctagt acacaagata ttgccttttt catgaaataa
     8161 caatcctccc ttaataaata tctaattttt catgaattta aatactataa gtttcagaca
     8221 ataaaaaaag acactcaaag agaatcttaa ttataaaata aaaagacttt actcttttta
     8281 gcaactggct gatatggctt taaaagcttt aatccctata gttttgcgtc attagatttc
     8341 cctaattttg ccatttaatt ttcactactt tcttacccat tttatactat atttaaactt
     8401 tttaatacaa tttttttaga ttaaattata aaaaaataac atttatatta atttattaat
     8461 ataaatgtta ttttccatta taatttaatt atttatatag cttccttaca agcataattt
     8521 tgtgtatatt ttcttattct ttatatcttc cataaaattc atgtgtagca tatagattct
     8581 tatctcctgt tatagctata ccaaatccca ttatttcata ccctttatgc aacatgttag
     8641 ttccttcttc tggatttgtc ttccactcat taaaaataaa gtttcctata tcatctgcat
     8701 ctctatttgt atatacaggt ttatctggaa ttttatattt aattatattt tcaccttgaa
     8761 ctgctatata tccaggttta atctcactcc aatctaagta tttcaaaaaa gtagaatatg
     8821 aattttttcc atttatcgta tgagataaag tcttcttatt tgccattaaa ttagaccaat
     8881 catttgctaa accctctaat ctgcttgcta cagataacgc tcttactcca ttctcttttc
     8941 tatactgctt tgtaagagca tatactttac cctttattct ctctttaaat gctctatcat
     9001 ttggattgta atataaacct tgttgatttg gtgaagcttg atttatacat tgcttgataa
     9061 cattctgatt tatgcctcct agtacagtca atttgtctgc accacttaaa atactcttgt
     9121 tactgccatt ttcaactaaa actacaggag cattttttgc tattgtagag gcagccaaag
     9181 cgtctgttaa ttgtaagcct ttacttagat aaaattcttt actattttta taaaactctt
     9241 gtataactat cttattagtt tcaaatctat ctgtgccacc caatctaata gaatttgtgc
     9301 tcttaactaa ttcgtcgctg attatttcac tagaaccaag cgcataactc tttaagcctg
     9361 tcgtgtcaaa tcccacacta tttccatctg taagtattat aggtactcca tctctagcag
     9421 ctactggtga tgcacttatc gcatctgctt ctcctgtaaa tccattggct ataattactt
     9481 tatctattgg cttaatttct ttgatttttt cagctatttt tatacttgta tcaaatctat
     9541 tttcaccaaa gattctttgt acaaccttct ttttggatag taactgattc tcaacatttt
     9601 tacttattgt tgattctgag cctattatat acacagtctt tgctttgttt aatctttcca
     9661 tagttgtgtt tggaatctga ttttgttttg tcatcaaaat aggcgcattt aaggctccag
     9721 ctaaaccact tgcacttaga ccatctgcta agctattatc tgagttgatt agtattgctg
     9781 tattgtagtt ttgcttatct gctatttttg ctgctgtctc atatctgtca ttccctttta
     9841 tttgctcaac tgatgtcaac gcatttacca taggagtatt taccattact aaaaatagtg
     9901 taagtcctat agctaatatc tttttactat tcttcatatt attttctacc cccaatgtac
     9961 ttttcatatt ttttattagt actcatactt atttttaatc aaaagttaca attttgttac
    10021 catattttat atatgatacc atagttatcc atacaactca aacaatatac ctaaagtttt
    10081 tattctctat
//
LOCUS       DAMJYR010000127        10070 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.132, whole genome shotgun sequence.
ACCESSION   DAMJYR010000127 DAMJYR010000000
VERSION     DAMJYR010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10070)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10070)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10070
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.132"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(54..524)
                     /locus_tag="PC471_003078"
     CDS             complement(54..524)
                     /locus_tag="PC471_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase/bleomycin resistance/dioxygenase
                     family protein"
                     /protein_id="HDF2790186.1"
                     /translation="MKYQVSLLAVKDIEVSKKFYKELFEQEIELDLGKNVTFKGGFAI
                     QEDFAWLTGINPDTIIQKSNNMELYFEVDDFDEFIEKLNYYKDVEFVHKPLKHEWQQR
                     VVRIYDPDKHIIEIGESMEVIARRYLSEGYSVEETSKIIQHPIKFVKQCENSKE"
     gene            complement(1394..1783)
                     /locus_tag="PC471_003079"
     CDS             complement(1394..1783)
                     /locus_tag="PC471_003079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HDF2790187.1"
                     /translation="MTIKKLPQSELKIMKFIWKSDSKVTSRDIVLGMEQKYQWKQTTT
                     LTLLSRLVVKRFLNSQKIDKYTHYEVLIKEKEYIGVETRDFFRNIHDSSIKSLLLSLH
                     ENINLSKDDILLIEEWIKNLKEEEKDV"
     gene            complement(3544..4950)
                     /locus_tag="PC471_003080"
     CDS             complement(3544..4950)
                     /locus_tag="PC471_003080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF2790188.1"
                     /translation="MDSVKYILRRFIGSSILISLLFIVLNIAGFLLFSYMLFVSKYSD
                     NLDTSDSREIKSNLIMIEDNLILRDGKFILDDSSKEVLHKNKVWAMLIDNNGDRIWGF
                     DLPKEIPSHYTLTDVAKFSRFFLKDYPVYVWEHPNGILVTGYPKDKYTKFNLTYTVKE
                     IEQFPMLIIGMFLVNAIVTFLIALFIGLKMVKSIKPIITGIKLMSKGEPVLLQEKGIL
                     SDISKSINTVSKELQMKDEKLRKKEEARSNWIAGISHDIRTPLSMIIGYAGELEESSS
                     LSNREQEQVSIICNQGIKIRELVNDLNLVSKLEYNMQTLNCDKIRVSAFLRELISEFI
                     NNNLDNDFYVELDILNEDIIIDADKKLLKRAISNLIQNSITHNQQGCNIRVTSKSDLD
                     FCYIIVEDDGKGMSQERINSLLKSQNGLSEAYAKGQNHGLGILIVSGIINAHRGKLDI
                     TSIDSQGLKTILKLPLIK"
     gene            complement(4995..5702)
                     /locus_tag="PC471_003081"
     CDS             complement(4995..5702)
                     /locus_tag="PC471_003081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2790189.1"
                     /translation="MESIKDKKILVVDDHKELLKMIDEILRKEGFSRVFLASSYEEAV
                     RVFRNVKPDCAILDVVLPDGDGFSIMRKIRETSKIPVIFLSARGEDEDRLIGLGLGAD
                     DYIVKPFLPKELTLRLVGILNRVYVPIEEEELPVFKLGDSVVVNLNSACVEKDGQEIS
                     LTAKEHSLLLKLYENKGRIVTNDSLCQAIWGDDMYGYENTLMVHVRRVREKIEKTPST
                     PKHLITVRGLGYKLMIN"
     gene            5873..6787
                     /locus_tag="PC471_003082"
     CDS             5873..6787
                     /locus_tag="PC471_003082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2790190.1"
                     /translation="MEMIIRTQRLSKEYNKIFRVKDIDLRVPQGAVYGFLGPNGAGKS
                     TTLKMLLGLAKPTQGNINILGKELNEKNRISILKDIGSLIESPSYYGHLTGLENMTIM
                     QRLLDLPKKNVNEALKIVRLENQKNKKVSQYSLGMKQRLGIAMAIMKFPKLLILDEPT
                     NGLDPAGIEEIRELIKSLPEKYGMTVLISSHLLSEIDQIATSIGIINHGELVFQDSIK
                     ELHNRGQSQIAIKTKNVVSAQKLLNTKGYMSLIQDGYLTIENLKDSDVAKINGMLVHS
                     NIDVFRIEEHKKSLEDIFLELTGKAVTL"
     gene            6787..7548
                     /locus_tag="PC471_003083"
     CDS             6787..7548
                     /locus_tag="PC471_003083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2790191.1"
                     /translation="MKYLGLEFYKLKRKKIILMTFLFAAVEIMWCIVNSNRAITRNPD
                     MLGGFEYSYMLMSFASLNGLFFPILISIITSRISDIEHKGDTWKLLKSSVTSLNSIYL
                     SKFLCSAILVSIPVLMQVLSIVLFGHFRGVIESISVSLLLKYTLGTILVSIAIIALHL
                     WIATVIPNQMIAITFGMIGSFIGLTSGLFYQGIRRLFIWSYYLELSPLSYAYDNVLGS
                     SVYRIDMNFSITILVFLIGMLIYYIGRNHLLKKEV"
     gene            7560..8297
                     /locus_tag="PC471_003084"
     CDS             7560..8297
                     /locus_tag="PC471_003084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2790192.1"
                     /translation="MLRKSVLGEILKLKNSFIWYVLLALPLISVLIGSGNFYLNQGIL
                     KKEWYSLWTQVSLFYGEFFFPILIAIFCAYICRLEHMNHNWNNVMTLPVNLKNIFLSK
                     LTVVSILTAITQVFFVIFYIVAGNMFGFSGSVPKELTGWIAKGWIISISIASIQLYMS
                     IKIRSFATPIGISLCLSLFGMGLIVAKPAVGIFFPYSMLGSAMGVIKQSALNVNDTIV
                     TICVAFITTIVFSEIANRNFRKNDVIS"
     gene            9175..9444
                     /locus_tag="PC471_003085"
     CDS             9175..9444
                     /locus_tag="PC471_003085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3795 domain-containing protein"
                     /protein_id="HDF2790193.1"
                     /translation="MFESRCGVCCDSCTKKEQVNCTGCPTMEKPFWGGECKVKTCCES
                     KELNHCGECDTFPCDMLLNKGKDQGFDPMVNIEQCRKWLEETVSN"
     gene            complement(9662..9907)
                     /locus_tag="PC471_003086"
     CDS             complement(9662..9907)
                     /locus_tag="PC471_003086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Pathogenicity locus"
                     /protein_id="HDF2790194.1"
                     /translation="MGELQKIPGVGKATEKSLIMLGYTTIKSLKDANPAQMYEKECLM
                     RGQHIDRCQLYVYRCAVYFASTENPEPEKLKWWYWKD"
BASE COUNT         3392 a         1561 c         1090 g         4027 t
ORIGIN      
        1 ttgaattagt tctattacaa ttaatttcta ctaaataatt ataatttaaa caatcattcc
       61 ttactatttt cacactgctt tacaaattta attggatgtt gaattatttt agaagtttct
      121 tctacagagt atccctcaga taaatatctt ctagctataa cttccataga ctctcctatc
      181 tcaataatat gcttatccgg gtcataaata cgcactactc tttgttgcca ttcatgtttt
      241 agtggcttat ggacaaactc tacatcttta taataattga gtttttcaat aaattcgtca
      301 aaatcatcaa cctcaaaata aagttccata ttatttgatt tttgtataat tgtatctgga
      361 ttaatccctg taagccatgc gaagtcttct tgtatagcaa atcctccttt aaatgttaca
      421 tttttgccta agtccaattc aatttcttgc tcaaataatt ctttataaaa tttttttgat
      481 acttcaatat ccttcactgc taataatgaa acctgatatt tcataaattt tcctcctaat
      541 tttttatttc tattttttca gtatcttcta ttaacatttt tttctctatc tcttcagtac
      601 cttttattaa tcctttattt ttactttttt agcatcttct attaacactt ttgtttctac
      661 ctattcagta tcttatatca atcttttatt ttttcaatat cttctattaa cacttttatc
      721 tctatctatt cagtactttc tatcaatctt ttgtttttat gttttcaata tcttctatta
      781 acacttttgt ttctacctct tcagtatctt atatcaatct tttatttttt caatatcttc
      841 tattaacact tttatctcta tctattcagt actttctatc aatcttttgt ttttattttt
      901 tcaatatctt ctattaatac ttttgtttct acctcttcag tatcttatat caatctttta
      961 ttttttcaat atcttctgtt aacactttta tctctatctc tttaatatct tatattaatc
     1021 ctttattttt attttttcaa tatcatcatt aattcctatt tattttatca tcttaaatac
     1081 gaaaacactg tgtcatattt gcaaaatata tattatcatc tatattaaag cttcacaatt
     1141 tatatttaaa aattctcttt aattacacta cctctattta aaaagctacc ttaattatac
     1201 ttataagtaa aggtagcttt ttaaatttaa ttaaatagaa tatccactat taatttatat
     1261 ttaatttatt tagattcaca ttatagtatc ataatccact tataatcatt tgtcatatta
     1321 cttaagtaga ctaatatatt acataaatac acttacatac actttatatt gatgctataa
     1381 gaaatacgtt tgcttatacg tctttctcct cttcttttag attttttatc cactcttcaa
     1441 taagtaaaat atcatctttg ctcaaattaa tattttcatg taatgacaac aataaacttt
     1501 ttatagaaga atcatgaata tttctaaaaa aatctcttgt ctcaactcct atgtattcct
     1561 tttctttaat taaaacttca taatgcgtat atttatctat cttttgagaa tttagaaatc
     1621 tttttactac taatctagat aaaagagtca aagttgtcgt ttgtttccat tgatattttt
     1681 gctccattcc taaaacaata tctctagatg ttactttgga atctgatttc catataaatt
     1741 tcattatctt taattctgat tgtggcaact tttttatagt catgataaaa ccttgctact
     1801 cagaatatag acaagcaata tccataataa aatttttcga ttaaatacat cttatacgga
     1861 tttatataag ctcagactta taagatattt ttcaatctta atcctaaatt tagctatttg
     1921 tacacggtaa atatatataa ataatgtcaa aattctcttg tattatattg cttaatttta
     1981 tgaaatattt tgtactcagc tatctatttt ggatacttgt ccagtagctc tcctttcttt
     2041 taaactcttt gattttaaat atttgtattt cagtaaatcc tacttcctaa attttttatt
     2101 tactacaact tattactgaa cttcctactt cctaaacttt ttatttacta caacttatta
     2161 ctgaacttct cacttactaa atttcttact tactataaat tataactaaa cttctcactt
     2221 actaaatttc ttacttacta taaattacta ctaaacttcc tactttctaa atttcttact
     2281 tgctacaaat tattactaaa cttcctctcc ctaaatttct tacttgctat aaattattac
     2341 taaacttcct actttctaaa tttcttactt actataaatt attactaaac ttcctactcc
     2401 ctaattttct tacttactat aaattattac taaatttcct actttctaaa tttcttactt
     2461 actacaaatt attactaaac ttcctactcc ctaaatttct tacttgctat aaattattac
     2521 taaacttcct actccctaag tttcttactt gctacaaatt attactaaac ttcctacttc
     2581 ctaaatttct tacttgctat aaattattac tactaaactt cccacttact aaatttctta
     2641 cttactacta cttataacta aacttcccac ttcctaaatt tcttacttac taccacttat
     2701 aactaaactt cccacttcct aaatttctta cttactacca cttataacta aacttcccac
     2761 ttcctaaatt tcttacttac taccacttat cactaaactt cctactttct aaatttcttg
     2821 cttcttaaga ataatgttaa ttttaatttt aagattatag tttttacctt gtttaattga
     2881 aacattctat ataattatat ccttctattt tagagttttt ttatttatat attacaaagt
     2941 ttaaattttt tcattaatta tattataaat tttccatata tttcaacata aatacatata
     3001 tatatatgtt atttctaaaa atactacaca aaaactctac tttagtagaa ctcaatggcg
     3061 caaaatactt caatttaaaa catttatcgt actttaaata aataactata ataaaatata
     3121 cttacaatag ttacatagaa gctattataa ctacacagaa cagcttataa ttaaaacttt
     3181 tttaggatgt cattatattt tttcttaaaa ttaaaaaaca ctataccata attctatttt
     3241 agtagaattt ttttgtcaat aataaaatta aatattaact aaaaaataaa aaaaatggaa
     3301 ttatctaaag aatttaataa ccttacaata attccaaaat attatttcta tgaaaatttt
     3361 gataccatga gtaactcagc cataagcgca taaagcacaa gttactcttt actttaataa
     3421 aacttgacaa gaaaagaatt tatccttatt ctactctaat agagttaatt tgtcaataaa
     3481 gaataaaatt attttcaaaa tattttcatt cttcattctt atctgttatt attttataaa
     3541 atatcactta attagaggta attttagtat tgtttttagt ccctgactat caatgctcgt
     3601 aatatctaat ttacctctat gtgcattaat aatgccagat actatcaaaa tacctagacc
     3661 atgattttga cctttggcat atgcctcact tagtccattt tggctcttta ataaactatt
     3721 aattctttct tgactcatac cttttccatc atcttcaaca attatatagc aaaaatctaa
     3781 atcagactta gaggtaaccc ttatattaca tccttgttgg ttatgagtta tactattttg
     3841 tattaaatta ctaatagccc tttttaataa ttttttatct gcatctataa taatatcttc
     3901 atttagtata tcaagttcaa cgtaaaagtc attatccaaa ttattattta taaactcaga
     3961 aataagctct ctcaaaaacg cagaaactct aatcttatca caattcaatg tttgcatatt
     4021 atattcaagt ttagacacta aatttaggtc atttactaat tctctgattt taatcccttg
     4081 attgcatata atagacactt gctcttgttc tctatttgaa aggctactgc tttcttcaag
     4141 ttctccagca tatccaatta tcattgaaag tggagttctt atatcatgag atattcctgc
     4201 aatccagtta gacctagctt cctctttctt cctaagtttt tcatctttca tttgcaactc
     4261 tttagataca gtattaatag attttgaaat atcacttagt attccttttt cttgtaaaag
     4321 tactggttct ccttttgaca taagctttat gccagtaata attggtttta tagatttcac
     4381 catttttaaa cctatgaata gagctattaa aaaagtaact attgcattaa ccagaaacat
     4441 acctattatt aacataggaa attgttctat ttccttaacc gtataagtaa ggttaaactt
     4501 agtatacttg tcttttggat aacctgttac taatattcca tttggatgtt cccatacata
     4561 cacaggatag tcctttaaaa agaaccttga aaactttgct acatctgtca aagtataatg
     4621 acttggaatt tcttttggta aatcaaatcc ccatattcta tctccattgt tatcaattaa
     4681 cattgcccaa actttatttt tatgtagtac ttccttacta gaatcatcta aaataaactt
     4741 tccatctctt aaaatcaaat tatcttcaat cataatcaag tttgatttta tttctctact
     4801 atcacttgta tctaaattat cagagtattt agatacaaat aacatatatg aaaacagaag
     4861 aaaacctgct atatttaata caataaaaag taatgaaatc aagatacttg aacctataaa
     4921 tcgcctcaat atgtacttta cactatccaa aattaaatca ctcctcttga tttcttttat
     4981 tctaatatct aaaattaatt aatcatcaac ttatatccaa gacctcttac tgtaataaga
     5041 tgtttaggtg tagatggagt tttttctatc ttctctctaa cccttctaac atgaaccata
     5101 agggtatttt catatccata catatcatct ccccaaatgg cttgacatag tgaatcatta
     5161 gtaacaattc tccctttatt ttcatacaac tttaaaagca acgaatgctc tttagctgtt
     5221 aaagaaatct cttgcccatc tttctcaaca catgcactat ttaaattcac aacaacagaa
     5281 tctcccagtt taaatactgg caactcctct tcttctatag gcacatatac acgatttaat
     5341 atacccacaa gccttaaagt aagttccttt ggtaaaaatg gctttactat ataatcgtca
     5401 gcccctaagc ccaaaccaat caatctatct tcatcttcac ctcttgcaga gagaaaaatc
     5461 actggtattt tagatgtctc tctaattttt ctcataatag aaaaaccatc tccatctggc
     5521 aatacaacat ctaaaattgc acaatctggc tttacatttc taaataccct aacagcttcc
     5581 tcataacttg atgctaaaaa tactctacta aatccctctt ttctcaggat ttcatctatc
     5641 atttttaata attccttgtg gtcatcaacc acaagtattt ttttatcttt tattgactcc
     5701 ataatcatca ccctccttta gtataccata tacccatttt cacaactttg cctaaataga
     5761 cttttaaggt tattgtaagg taaatttcag ctaattgaaa tgtaaccaag ttattatact
     5821 gatataagtt aaaagaatca ataagcacag attaaaaaag gggatgaaac atatggaaat
     5881 gataattaga acacaaagac tttcaaagga atacaataaa atatttagag taaaggatat
     5941 agacctaaga gttccacaag gagctgtata tggttttttg ggtcctaatg gagctggaaa
     6001 atctaccact cttaaaatgc ttcttggact tgctaaaccc acacaaggaa atattaatat
     6061 tttaggtaaa gagttaaatg aaaaaaatag aatatcaatt ttaaaagata taggttcttt
     6121 gatagaatct ccatcatact atggtcatct tacagggctt gaaaacatga ccataatgca
     6181 aagactctta gatttaccta aaaaaaatgt aaatgaagca ttaaaaattg taaggttaga
     6241 aaatcaaaaa aataaaaaag ttagtcaata ttctttaggt atgaagcaac gtcttggcat
     6301 tgctatggca ataatgaagt ttcccaaatt attaatactt gatgaaccta caaatggact
     6361 cgacccagca gggattgagg aaattagaga attaataaaa tctctaccag aaaaatacgg
     6421 gatgactgtt ctaatctcta gccatctttt aagtgaaatt gaccaaattg ccacatcaat
     6481 aggaataata aatcatggcg aactggtttt tcaagatagc ataaaagaac ttcataatag
     6541 aggtcaaagt cagattgcta tcaaaactaa gaatgttgta tctgcacaaa aactattaaa
     6601 tactaaagga tatatgtcac ttatacaaga tgggtacttg actattgaaa atttaaaaga
     6661 ctcagatgtc gctaaaataa atggtatgct tgtccattca aatatagatg tatttagaat
     6721 agaagaacat aaaaaaagct tggaagatat attcttagaa cttactggta aggcggtgac
     6781 tttataatga aataccttgg tctagaattt tataaactta aaagaaagaa gattattctg
     6841 atgacctttt tatttgcagc tgttgaaata atgtggtgta tcgtaaattc aaatagagct
     6901 attacacgta atccagatat gttaggtgga tttgaatatt cctatatgct tatgagtttt
     6961 gcaagtttaa atggtctatt ctttcctatc ttaatatcaa taattacatc acgtataagc
     7021 gatatagaac ataaaggaga tacatggaag cttttaaaat cttctgtgac atctttaaat
     7081 agtatttatt tatctaaatt tttatgttct gctattttgg tatctatacc tgtattgatg
     7141 caagtgttat ctattgtgtt gtttggacat tttagagggg ttattgagtc aatttctgta
     7201 tctttacttt taaaatacac attaggaaca attcttgtaa gtattgctat cattgcacta
     7261 cacctatgga ttgcaactgt aattccaaat caaatgattg caattacatt tggcatgatt
     7321 ggtagtttta taggtttgac atcaggtttg ttttatcaag gtattcgtag attgtttata
     7381 tggtcttatt acctagagtt atcaccttta agttatgctt atgataatgt tttaggttca
     7441 tctgtctata gaattgatat gaatttttct attacgattt tagttttctt aattggaatg
     7501 ctaatttatt atattggaag aaatcattta ttaaaaaaag aagtatagga gggatttaaa
     7561 tgttaaggaa gtcagttttg ggagaaattc taaagttaaa aaattctttt atatggtatg
     7621 tattattagc tttgccactt ataagtgtgt tgattggttc tggtaatttt tatctaaatc
     7681 aaggcatact caaaaaagaa tggtacagct tatggactca agttagtctt ttttatgggg
     7741 aatttttctt tcctatctta atcgcaatct tttgtgcata tatttgtagg cttgaacata
     7801 tgaatcataa ttggaataat gtaatgacac tgccagttaa tttaaaaaat atctttttat
     7861 ctaaacttac agtagtttca atacttacag ctattacaca agttttcttt gttatctttt
     7921 atatagttgc aggaaatatg tttggttttt ctggtagtgt acctaaagaa ttgactggtt
     7981 ggattgctaa aggttggatt atttctattt caatagcttc tatacaatta tacatgtcta
     8041 taaagattag aagttttgca actcctattg gtattagtct ttgcctttct ctatttggta
     8101 tgggattaat agtagcaaaa cctgctgttg gtatattttt cccttattct atgttaggta
     8161 gtgctatggg ggttataaag caatctgctt taaatgttaa tgacactata gttacaatat
     8221 gtgttgcatt tattactact atagtatttt ctgaaattgc caatagaaat tttagaaaga
     8281 atgatgttat ttcttaatgg taaatgctag atatttttgg gtttgaaatt tatattcttt
     8341 ttagttgaaa tctatatcca ttttatttaa aaatatatat cttttatttg aattttatat
     8401 gttttatcta ctcgctatat aatagatata ctcctaaaat ttaaaaatat ataataatgt
     8461 tattatttaa aattattatg tattatatta aaacttaatg caaaaacaat ttttatatta
     8521 tataatgaaa cttaattaaa tataggtaaa aaatcattca atatattaaa taaatattta
     8581 actttaataa tctatattta attatagcta taacatagaa ttatcaaata tattgaatga
     8641 atatttgact cttggaatgt gtaattaaat taagtttggt tattcgatgg aaggcaaatt
     8701 tgatataaaa ctccatttaa agtgccagat aaaaacttta tggttatata aaagttaaaa
     8761 gtaatttata gtgttataga acattcaaag ggatttaaag taatctatgg tgttttgaaa
     8821 tattcaaagg gatttaaact aatctatagt cttgtagaat attcaaagag atttaaaaca
     8881 atctataatg ttgtaaaata tattcaaagg gatttaaact aatctatagt cttgtagaat
     8941 attcaaagag atttaaagca atctataatg ctgtaaaata tattcaaggg gatttgaagt
     9001 aatttattgt gttttgaaat attcaaaaag atttttggaa tagtttttat aaatttacca
     9061 gtactaataa ttttatgttt aacatttaga tgacctaaaa tcaactattt gcacttttgt
     9121 attcatctaa aatcatttta taatattttt aaataatgga aaggatgttt gaatatgttt
     9181 gaatcaagat gtggtgtctg ttgtgacagt tgtacaaaaa aagaacaagt aaattgcact
     9241 ggttgcccaa ctatggaaaa accattttgg ggtggagaat gtaaggttaa aacttgctgt
     9301 gaaagtaagg aacttaatca ttgtggtgaa tgtgatactt tcccttgtga tatgctttta
     9361 aataagggaa aagaccaagg ttttgaccct atggtaaata ttgagcaatg tagaaaatgg
     9421 cttgaagaaa cagtttctaa ttaattgttt tagaatgctt tgttaaggaa tttatttaat
     9481 cctttgttag gtagtatgtt tagtcatttg ttgattagta tatttataca tttattgatg
     9541 ggtatattta gccatacaaa tagatttaca taaaatttac gactaaaaaa gagttgagta
     9601 ttagttttta ttatttaaaa cggatattca actctttttt atgtctaact tttttgattt
     9661 tttaatcttt ccaataccac cactttaatt tttctggttc tggattttca gttgatgcaa
     9721 aatatactgc acatctgtat acatataact gacatctgtc tatgtgctgt ccacgcatta
     9781 aacattcctt ttcatacatt tgtgctgggt ttgcatcttt aagtgattta atagttgtat
     9841 atcctaacat gattaaagat ttttctgttg ctttccctac ccctggaatt ttttgtagtt
     9901 ctcccatact tcaccttctt tctggtatat tctttttata ttatacctat taccagatat
     9961 ttttttatta tagttaatat ttaatattta attttttatt atatcaattt atttatgctc
    10021 tttcatatat atatatttta ccacctctaa atcactgagt cacttaatta
//
LOCUS       DAMJYR010000128        10028 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.133, whole genome shotgun sequence.
ACCESSION   DAMJYR010000128 DAMJYR010000000
VERSION     DAMJYR010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10028)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10028)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10028
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.133"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(15..203)
                     /locus_tag="PC471_003087"
     CDS             complement(15..203)
                     /locus_tag="PC471_003087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2790195.1"
                     /translation="MESGLFVSRILCVVLSFPHFKKIADGFTDEIALYFPHKTNIYLK
                     IFLRFLLLNYILNKENIK"
     gene            699..857
                     /locus_tag="PC471_003088"
     CDS             699..857
                     /locus_tag="PC471_003088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2790196.1"
                     /translation="MRITKGKIKELIELCHQLEKLFVQVFKSLEHILISLCSCIGWLL
                     ILIYIIKH"
     gene            1110..1301
                     /locus_tag="PC471_003089"
     CDS             1110..1301
                     /locus_tag="PC471_003089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2790197.1"
                     /translation="MKLEKLIDIRKEKKYSQEDIARMLDISLRNYHRKEKGENQFTVS
                     ELEKICSYLDIESKELLDI"
     gene            complement(1483..1794)
                     /locus_tag="PC471_003090"
     CDS             complement(1483..1794)
                     /locus_tag="PC471_003090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2790198.1"
                     /translation="MNRLRELRKEKGYSTQQVGELLGVHYVTIQNYETNRRKIDNETL
                     IKLSNIYNVSIDYILCLTDNRENIQLEEEEKQLLNNYRELDTKSKTIVQEQVNTLKKL
                     L"
     gene            2526..2849
                     /locus_tag="PC471_003091"
     CDS             2526..2849
                     /locus_tag="PC471_003091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2790199.1"
                     /translation="MDEKILELLQKMDTSINDIQGEIKWINTRLDSMDGRFDGIENQL
                     SDLKEGQEEIKKKLDLTYNQVARNMEGITEVGEKIDTLKNDMNFVEMATSKNWNEIAK
                     LKSIK"
     gene            2944..3132
                     /locus_tag="PC471_003092"
     CDS             2944..3132
                     /locus_tag="PC471_003092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2790200.1"
                     /translation="MYEQRKAKVIFNKGAGNSRGESYTNRVTIPTTWIKHMDITKLDR
                     EVLLTFDGNKIVIEKIDE"
     gene            3254..3967
                     /locus_tag="PC471_003093"
     CDS             3254..3967
                     /locus_tag="PC471_003093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HDF2790201.1"
                     /translation="MLNIYITNLGKYNEGELIGEWAELPINEEELQEILDRIGINEEY
                     EEYFITDFETDIEGLEISEHSNIEQLNELAMKLERLEEYEIEELKALLERGYIDFYDM
                     IESDICSLSDNYTFIQLDDRSQLDDENLGRSYIEEIYCGDLSYIENIAYYFDIEAFSR
                     DLRFDKDMIIENLYEEDIEYYENMTDIEFAENYIERLGDIEELGQETLERYFNYELYG
                     TDLRYENICIASNYLAIVS"
     gene            4035..4175
                     /locus_tag="PC471_003094"
     CDS             4035..4175
                     /locus_tag="PC471_003094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2790202.1"
                     /translation="MKKLIIKIKSLFKKDLDSSYWSTTESIILKCGNSNQLELLKQAN
                     KL"
     gene            4188..4424
                     /locus_tag="PC471_003095"
     CDS             4188..4424
                     /locus_tag="PC471_003095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2790203.1"
                     /translation="MVNYSEYMTATDLINICDLLEVKIIGEAGTYNSLYVRLGNLIGL
                     DWALKGGELRQQLIQNINNMMYLDNKNKMQYKEF"
     gene            4459..4755
                     /locus_tag="PC471_003096"
     CDS             4459..4755
                     /locus_tag="PC471_003096"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2790204.1"
                     /translation="MKKIINGYSIYKDGTWCEVIKEGKFIFAGSVNEKLSYEDIYIAL
                     TAKLEFRLHGKLLLSYSLYGTFQGEKQSTLELLAYENNCNINDIEVKQVVENIQ"
     gene            4892..5437
                     /locus_tag="PC471_003097"
     CDS             4892..5437
                     /locus_tag="PC471_003097"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2790205.1"
                     /translation="MKKIANLNKEELKIIWEKNSQLREDVRKACEDNDMYWIDEILCH
                     LNKSLDDWSIGFYNDNYIKIEDNHKFLLELKSVCEKFSFLSEEEQRPLEYAISLIDKL
                     HCMDFENKRFNMLENKINNMVKKLEEKVIEEFNKMTEPLDKEYLLENFIEFYVEAYLN
                     DNEFYIDNEYVLYEKIIKSYA"
     gene            5463..5726
                     /locus_tag="PC471_003098"
     CDS             5463..5726
                     /locus_tag="PC471_003098"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2790206.1"
                     /translation="MLANQIKGIKTSLETSSLRELKGYIKDLRKALSGYKYKNKIDEI
                     TEYQIDKLCYKIDNTDIFDLKYKEIEKYIDSLILILDFIFMEM"
     gene            5764..6348
                     /locus_tag="PC471_003099"
     CDS             5764..6348
                     /locus_tag="PC471_003099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2790207.1"
                     /translation="MNEYILISYTKGEGTCDHCGRTIKNIATIKNNVTGEVFHVGLTC
                     VEKIMKLNVTFYKALSREIKKHYKCMEYYSKGLDIETNLNKIVKNNTKYKEGSYAYKS
                     NEQMLEDAIAEVAWSLARMIDSCMRMNKLSKSGLIDIDILNSLFIKYKEYMSKFDENY
                     NKNKYKYKSCYTMKPLKVILDENEDLKELYKSMQ"
     gene            6652..7173
                     /locus_tag="PC471_003100"
     CDS             6652..7173
                     /locus_tag="PC471_003100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2790208.1"
                     /translation="MKFKNIVMGMLLGLIIVGVSGVTKVKAAELDNFYIESTDRVIEV
                     LEDESIVLYDTKEQVYNFYPICMGDWNYSFKNKKDLDSAVATYKELSSNINHLSNIYV
                     VNKLNDNGNIKVFLNDGSSIVYIKQDNKYYFYPSCMGDWYLTLDSKIYLTNCINTYIE
                     YYYSLSTGGGTNE"
     gene            7166..7396
                     /locus_tag="PC471_003101"
     CDS             7166..7396
                     /locus_tag="PC471_003101"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2790209.1"
                     /translation="MNNNDKLSILNQLEYIENDIDARNEMIEKFKDLLVKYPKSRDIR
                     RYIKQQTQFLNGIRYIYDNLDILKNMINNDKK"
     gene            7567..8376
                     /locus_tag="PC471_003102"
     CDS             7567..8376
                     /locus_tag="PC471_003102"
                     /inference="COORDINATES: protein motif:HMM:NF022027.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6C domain-containing protein"
                     /protein_id="HDF2790210.1"
                     /translation="MNKLKVKGKQKFMNLEIPVIEGGFGEDKKCMTDKTISEIHNQPN
                     FKIRERIKDNIKRFKEDIDYIDLKVIRDTDDNLELLQTLGYSKMQISKADNIFLLSER
                     GYSKLIKIMDTDLAWEIHDKIMDEYFNMRKKLNDPFENLSPELKVIIKLEQRQAELES
                     GQHELEEKVNVLYDFSTIDYNQQETIRSLVNRMVIQVYQCLNFSAYEDAKLRKRVYFS
                     LWKDYRKKFGVNSYKNTAKKNYDNAIKFIENWEPNYDLSNEIYMFQKVENI"
     gene            8654..9370
                     /locus_tag="PC471_003103"
     CDS             8654..9370
                     /locus_tag="PC471_003103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011948617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HDF2790211.1"
                     /translation="MNILIENKFIEKLKYTKNIPNSLYKDLRHNFLFHSNKIEGSTFT
                     SESLQDLIEHNIVKGDHTLDDVITTVNSSYVFETVIDTLGEYLTEKYIKDLHGQLMYN
                     SNIHNMGLSGIYKKIPNRIIGTNSVLAQPFEVEPKMLELIEWYNNLKDITLKDLAEFH
                     YRFEIIHPFQDGNGRIGRFILLKQILENNLKLIIISSDTVDDYRQALKGCHNEYYKPL
                     EQYFESLDDYKEKNKDLWSF"
     gene            complement(9612..9911)
                     /locus_tag="PC471_003104"
     CDS             complement(9612..9911)
                     /locus_tag="PC471_003104"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2790212.1"
                     /translation="MVIKVNFGGNYSPDYIYVPNEISLKKYEIRNEFHIWIDNTEDEH
                     PFWCYENGVQIGLHYRGDAIVYWLNNYILKNANDKARLIESFSNKKLDYDLIINL"
BASE COUNT         4177 a         1013 c         1494 g         3344 t
ORIGIN      
        1 caatacatac acttttattt tatattttcc ttgttaagaa tgtaatttaa gagtaaaaat
       61 ctaagaaata tctttagata aatatttgtt ttatgaggaa aatacagagc tatttcgtct
      121 gtaaatccgt ctgcaatttt tttgaaatgt ggaaatgaaa ggactacaca caaaatccta
      181 ctgacaaaca agccactttc cataaaatac cccctacttc atttttattt attttaatat
      241 gtaaaacact ctatttactt tatttaatta ataaaagtat tacttttatc ttattttaaa
      301 aatctatgtt tttgtcagca aaaaatggca taaaattaag tattttatgc catttttcta
      361 aaacctagta ttttaaacac ttacaacacc tacaccttct attttgtcag taaaatgtca
      421 gtcattataa ttcttagact acaaacctgt tgtaatcact gcatttcaat ttttagttat
      481 tgatatctat ataaaatatt atttgctata gaataaattt ttttgttatg gattataaag
      541 tcttatatac atcattttag cattaatctg acatcgtcga atatcaactc acaacctcat
      601 tgccatattt ttcttgacaa atttgtcatt attgtatata atttaattaa gataagaatt
      661 aatcttatct atacaattca atattagggg gtgacgttat gagaataaca aaaggcaaaa
      721 taaaagagct aatcgagttg tgccaccaac ttgaaaagct ctttgtacaa gtatttaaat
      781 cacttgaaca tattttgatt tctttatgtt cttgtatcgg ttggttatta atacttatat
      841 acataattaa acattaataa ccaatgcaat atacctataa aaatatatct ctataggtat
      901 attgtaacac acctaatatt gaaagtaaac gaggtgacga catgagtcaa catattaaaa
      961 acactttaaa aataataaag atactaggtt tattattctt cttatatttt gcacttagag
     1021 caatatatat tgtttatctt taatatcttt attgagttgc ttaatgtata agtaactctt
     1081 ttttgttata atattaatga ggtgatagca tgaaattaga aaaattaatt gatataagaa
     1141 aagaaaaaaa atattctcaa gaggatattg ctagaatgtt agacatatct ttaagaaatt
     1201 atcacagaaa agaaaaagga gagaatcaat ttacagtaag tgaactagaa aaaatatgta
     1261 gctatttaga tatagaatca aaagaactac ttgacatata gtaaataaaa taatttaata
     1321 atataaaata gactagctat aatctagtct attttattat aataacatat tgtacaagcc
     1381 atttaaatcc attctaagca catttcaatg cattcacctt atgtttatac ctttaatctt
     1441 taaaattaaa tataaaaata agctagacat atatactcta gcttacagta atttttttaa
     1501 tgtgttgact tgttcttgta ctattgtttt actcttagtg tctaactctc tataattatt
     1561 taatagttgt ttttcttctt cttctagttg tatattttct ctattgtctg taagacataa
     1621 tatataatct atacttacat tatatatatt agataatttt ataagcgtct cattgtcaat
     1681 ttttcttctg tttgtttcat agttttgtat tgttacataa tgtacgccta atagttctcc
     1741 cacttgttga gttgaatatc ctttttcttt tcttagctct ctcaatctat tcattaaatc
     1801 acctcttaat tctaaatata acatattata aatttaatta caattgtatt caaaacggta
     1861 tgttttatat atgacattat ttaaaacatt ttgttttgtt ttttatattt tatattgaca
     1921 atcaaaacat tttgttttat aattaaatca tagcaaggga gaacaagaaa acacaaagcc
     1981 acctctcaaa tgaagtaaca ggcaaagaac cttcttaaaa ctctagctaa tatcttaaag
     2041 ggttctatta aaaaatatta aactactatt aaaaaattac tacctttaag ttgacctttt
     2101 aaatggtgtg aatggtgaaa ggcacacaac acaaatattg cacatagttt tttcttcatt
     2161 ttttaactac aaaaatgaat tagtcatacc agttggtgag gtgttcccaa cattaagtaa
     2221 ttaagtaaat aaaattaata aaggtttcca attaaagcct acaaaaaatt gaggtatcaa
     2281 cttagttgct tctataatgg cactcaatac aatttaatat gacatagcag ttgtgaggta
     2341 ctgcgttata aaaagtacta aagattgagc attaaaatta tagtttgtac tgcttgacac
     2401 ctacatggtt ataaactatc taatatttac tagtataatt taaactattt attagattat
     2461 tttagtaagt gttagaatac aacacttaaa ttatgatata ataataaaat aggagtgtga
     2521 acggcatgga tgaaaaaata ttagaattat tacaaaaaat ggatactagt attaatgata
     2581 tacaaggaga aataaaatgg attaatacta gactagatag tatggatggt cgttttgatg
     2641 ggattgaaaa ccaacttagt gatttaaaag aagggcaaga agaaattaaa aagaaactag
     2701 acttaactta taatcaagtt gctagaaaca tggaaggcat aacagaagtt ggagaaaaaa
     2761 tagacacttt aaaaaatgac atgaactttg tagaaatggc tacatctaaa aattggaatg
     2821 aaatagcaaa gttaaaatca attaaatagt aatacataaa aagacacttg aattattgag
     2881 tgtcttttct acaagctctt agtatggtat tattaaacaa taatatcatt ggaggtataa
     2941 cgaatgtatg agcagagaaa agcaaaagta atattcaata aaggagctgg taattctagg
     3001 ggagaatcat atacgaatag ggttacaatt ccaactacat ggataaaaca tatggacata
     3061 actaaactag acagggaagt tttattgact tttgatggta ataaaatagt aatagaaaaa
     3121 atagatgagt agataaaaaa tctactcaaa aaaatacttg acaactcgtg acgagtcgat
     3181 tataattaaa tcataaaata aaatttttta caaaatgact agtaacgagt cagtaaaaag
     3241 ggaggatata aaaatgctaa atatatacat aactaattta gggaaatata acgaaggtga
     3301 actaataggg gaatgggctg aacttccaat aaacgaggaa gaattacaag aaatattaga
     3361 tagaataggg ataaatgaag aatatgagga gtatttcata acagattttg aaacagatat
     3421 agaagggtta gaaataagtg aacatagcaa tatagagcaa ctgaatgaat tagcaatgaa
     3481 acttgagagg ttagaagaat atgagattga agagttaaaa gctttacttg aacgtggata
     3541 cattgatttt tacgacatga ttgaaagtga tatttgtagc ttatcagata attatacttt
     3601 tatacaactt gacgacaggt cacaattgga tgatgaaaat ttaggacgta gttacataga
     3661 agaaatatat tgtggtgatt tatcttatat agaaaatatt gcttactact ttgacataga
     3721 agcattttca agagatttac gttttgataa agacatgatt attgaaaatt tatacgaaga
     3781 agatatagaa tattatgaaa atatgacaga tattgaattt gcagagaatt atattgaacg
     3841 attaggagat attgaagagt taggtcaaga aacacttgaa agatacttta attatgagct
     3901 atatggaact gacttacgtt atgagaatat ttgtatagca tcaaattatt tagctatagt
     3961 ttcttaaata aaaagtttta gggcgttctt taaaaacgcc acttccaata aaaaattgaa
     4021 aggggaataa aattatgaaa aaattaataa tcaagataaa atcattgttt aaaaaggatt
     4081 tagatagtag ctattggagt actacagaaa gtattatttt aaaatgtgga aacagcaatc
     4141 aattagaatt attgaaacaa gctaataaat tataaggagg taataacatg gtcaattatt
     4201 ctgaatatat gactgcaaca gacttaataa atatatgtga ccttttagaa gttaaaataa
     4261 taggagaagc aggaacttat aattctttat atgtcagact aggaaattta atcggattag
     4321 attgggcttt aaaaggagga gaattgagac agcaattaat acaaaatatt aataatatga
     4381 tgtatttaga taataaaaat aaaatgcaat ataaagaatt ttaagtaaaa aataattaat
     4441 ttataggagg aaaataaaat gaaaaaaata ataaatggat attcaatata taaagatgga
     4501 acatggtgcg aagtaataaa agaaggaaaa tttatctttg ctggttctgt aaatgaaaaa
     4561 ctatcatatg aagatatata tatagcttta actgcaaagt tagaatttag attacatggt
     4621 aagttactac tttcatatag tctgtatggt acatttcaag gggagaaaca gtccacatta
     4681 gaacttttag catatgaaaa taattgtaat ataaatgaca ttgaagttaa acaagttgtt
     4741 gaaaatatac aatagtctgc aaattcaaaa aggaaacaaa atattaaatt taatagatta
     4801 cataagttac tatcattgtc gatataaagt tatatattaa ttgataatga tagtaatttg
     4861 ataaaacaaa catattaggg gaggtataaa aatgaaaaaa atagctaatt tgaataaaga
     4921 ggaattaaaa ataatatggg agaaaaatag tcaactaagg gaggatgtaa ggaaagcttg
     4981 cgaagacaat gacatgtatt ggatagatga aatattatgc catcttaata aatcattaga
     5041 tgattggtct attggatttt acaatgataa ttatataaaa atagaggata atcataaatt
     5101 tttattagaa cttaaatctg tatgtgaaaa attttctttt ttatcagaag aagaacagag
     5161 acctctcgag tatgcgattt cattaattga taaattacat tgtatggatt ttgaaaataa
     5221 aagatttaat atgttggaaa ataaaattaa taatatggtc aaaaaacttg aagaaaaagt
     5281 aattgaagaa tttaataaga tgacagagcc attggacaaa gaatatttat tagaaaattt
     5341 tattgaattt tatgttgaag cctatctcaa tgataatgaa ttttatatag ataatgagta
     5401 tgtgttatac gaaaaaatta ttaaaagtta tgcttaaaag aatgtaattg gagggtttta
     5461 aaatgttagc aaatcagata aaaggtatta aaacatcact tgaaacaagc agcttaagag
     5521 aactaaaagg atacataaaa gatttaagaa aagcactatc aggatataaa tataagaata
     5581 aaatagatga aattacagaa tatcaaatag ataaattatg ttacaaaatt gataatacag
     5641 atatatttga cttgaaatat aaagaaatag aaaagtatat agatagttta attttgatac
     5701 ttgattttat ctttatggaa atgtaatcaa aagaatgatt ttaatataac tggaggttta
     5761 agcatgaatg agtatatttt gataagttat acaaaaggcg aaggaacttg tgaccactgt
     5821 ggaagaacta taaaaaatat agctacaata aaaaataatg ttacaggaga agtttttcat
     5881 gttggattaa catgtgtaga aaaaataatg aaattgaatg ttacatttta caaagcattg
     5941 tcgagagaga taaaaaaaca ttacaaatgc atggagtact acagtaaggg tttagatata
     6001 gagacaaatc ttaataaaat agtgaagaat aatactaaat acaaagaagg ttcttatgct
     6061 tataaatcta atgaacaaat gttagaagat gcaattgcag aagtggcttg gtctttggct
     6121 cgtatgatag atagttgtat gagaatgaat aaacttagta agtctggatt gatagatata
     6181 gatatattga acagtttgtt tatcaagtat aaggaatata tgagtaaatt tgatgaaaat
     6241 tacaataaaa acaaatataa atataaaagt tgttatacta tgaaaccttt aaaagtaata
     6301 ttagatgaaa atgaagattt aaaagaacta tataaaagta tgcaataaat gtatgtaggc
     6361 gatgaattaa tgacaatgaa ttttatatag atgaggatta tattttatat aaaagagtgt
     6421 catatcttta atataggaca tgaagtaata aaagagttta aaattatatt tttaatatga
     6481 tatatgagtt atcaaatcac tatcattata gctatattat tataatttaa atgataatga
     6541 tagtgatttg ataaaaaggt tttaaagatt gtaaaaatac attaagatga ttttcaaaca
     6601 gcttatagag gcttgtacgg ggtcataatt ataaactagg aggatataaa aatgaaattc
     6661 aaaaatatag ttatgggaat gttattaggt ttaatcattg taggagtttc aggagtaaca
     6721 aaagttaagg ctgctgaatt agataatttt tatatagagt ccacagatag agttattgaa
     6781 gttttagaag atgaaagtat agttttatat gatactaaag agcaagttta taatttctat
     6841 ccaatttgta tgggagactg gaactatagc tttaaaaata aaaaagattt agatagcgca
     6901 gttgcaactt acaaagagct atcaagtaat ataaatcatt taagtaatat atacgtggtt
     6961 aataagttaa atgacaatgg aaacattaaa gtatttttaa atgatggaag ttcaattgtt
     7021 tatataaaac aggataataa atattacttt tatccttctt gtatgggtga ctggtactta
     7081 acattggata gtaaaattta tttaactaat tgtattaata cttatataga atattattat
     7141 tccttgtcaa ctggaggtgg cacaaatgaa taataatgat aagttatcta tattaaatca
     7201 attggaatac atagaaaatg atattgatgc aagaaatgaa atgattgaaa aatttaagga
     7261 cttattggtc aaatatccaa agagtagaga tataagaaga tatataaaac aacaaactca
     7321 atttttaaat ggtattagat atatatatga taatttagat attttgaaga atatgataaa
     7381 taatgacaag aagtgagaac attactagac ataacaatgt ctactgaatg tcaaagagat
     7441 tcatttagta aatacaaagt tattatgtca atttttaatt aaattgtaat ttaacataaa
     7501 aatgtagtta ctattttaaa actcataata actacattta ataatttaaa aagggagatg
     7561 gaaaaagtga ataaattaaa agttaaagga aaacaaaaat ttatgaattt agaaatacca
     7621 gttatagaag gtggttttgg tgaagataaa aaatgtatga cggataagac tatatcagaa
     7681 atacataatc agcctaattt taagataaga gagagaataa aggataatat aaaaagattt
     7741 aaagaagaca tagattatat agatttaaaa gttatccgtg acacggatga caatttagag
     7801 ctattacaaa cacttggata tagtaaaatg caaatatcaa aagctgataa tatattttta
     7861 ttatctgaaa gaggatattc aaaattaatt aagataatgg atactgattt agcttgggaa
     7921 atacacgata aaataatgga tgaatatttc aatatgagaa aaaagttaaa tgaccctttt
     7981 gaaaacttat ctccagagtt aaaagttata atcaaactag aacaaagaca agcagaactt
     8041 gaatctggtc aacatgaatt agaggaaaaa gttaatgtcc tatatgattt ttctactata
     8101 gactacaatc aacaagaaac tattagaagt ttggtaaata gaatggtaat acaagtatat
     8161 caatgtttaa atttttcagc ttatgaagat gctaaattaa gaaaaagagt atacttttca
     8221 ctatggaaag attatagaaa aaaatttggt gttaatagct ataagaacac tgctaaaaag
     8281 aactatgaca atgcaattaa attcatagaa aactgggagc ctaattatga tttaagcaat
     8341 gaaatataca tgtttcaaaa agttgaaaat atataagaat aatgattgga gattattatg
     8401 atatgttatg tcaaagatat acatactata agcctatatg atgcttttag atggcataca
     8461 atggggttgt cattcattat taaagatggt aaattaaaag gttttacaat gtaactaatt
     8521 aattgagaag agaattatga taacattctt ttctcaatta attatcattc ctaataaata
     8581 aagttcaaaa gatagtgtaa aagataatac attttgttaa tattagtaat ataaaataaa
     8641 tttaaaaatg gagatgaata tattgattga aaataagttt atagaaaaat taaaatatac
     8701 caaaaacata ccaaactctt tatacaaaga cttaagacat aattttttat ttcattcaaa
     8761 taaaatagaa ggaagtacat tcacaagtga aagtttacaa gatttaattg agcacaacat
     8821 tgtaaaaggt gaccatactt tagatgatgt tataacaaca gtaaattcaa gctatgtatt
     8881 tgaaactgta atagatacat tgggcgaata cttaactgaa aagtacataa aggatttaca
     8941 tggacaactt atgtataatt caaatattca taatatgggg ctatctggta tatacaaaaa
     9001 aataccaaat agaataatag gaactaatag tgtattagcc caaccatttg aagtagaacc
     9061 aaagatgtta gaacttattg agtggtataa taatttaaaa gatattacat taaaagactt
     9121 agcagaattt cattatagat ttgaaatcat tcatcctttt caagatggca atggaagaat
     9181 aggaagattt atattactta aacaaatact tgagaataat ttgaaattga ttataatatc
     9241 gagtgatact gtagatgatt atagacaggc tttaaaaggt tgtcataatg aatattataa
     9301 acctttagag caatattttg aaagtttaga tgactacaaa gaaaaaaata aagatttatg
     9361 gagtttttaa cctaataatt aaacaaatga atggaagtga aaactatgct ttacagaacg
     9421 gaagatttaa atacttttgc aggtattgga agttctaaag gtgtttattg tcaactgcca
     9481 ctaccctaaa gggtagtagc ttgtaaaagc tagttgaata gcctaagttt acactacgtt
     9541 atgtaagaat atatagctac tttagaatac tttaaactta aaggaatatt ccacatatta
     9601 aaatactctt tttacaaatt tataattaaa tcataatcta actttttatt tgaaaaactt
     9661 tcaattaacc ttgctttatc attagcattc tttaaaatgt agttatttaa ccaataaact
     9721 atcgcatcac ccctataatg gagtccaatt tggactccat tttcataaca ccaaaaagga
     9781 tgctcatctt ctgtattgtc aatccaaata tgaaattcgt ttctaatttc atattttttt
     9841 aaactaattt cattaggaac atatatataa tcaggactat agttgccacc aaaatttact
     9901 tttataacca taattatagt ctccttttta aataattata acatatttta taaaaaactg
     9961 gtaattacac ttgtagttta attccaaaat atagcattct acaagtgtat ctaaatttat
    10021 taaagcaa
//
LOCUS       DAMJYR010000129         9797 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.134, whole genome shotgun sequence.
ACCESSION   DAMJYR010000129 DAMJYR010000000
VERSION     DAMJYR010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9797)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9797)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9797
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.134"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            611..1513
                     /locus_tag="PC471_003105"
     CDS             611..1513
                     /locus_tag="PC471_003105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2790213.1"
                     /translation="MSGIISVMTQSLILSIMALGVYITYKILDFPDMSADGSYTMGAS
                     IVAFSLTNGISPVVATLMAILCGCTAGLVTGILHIKFKISNLLSGILVMGMLYSINLR
                     IMGKSNIPLFSFKHLFNGEISPIVLALAFVFICKVLLDLFLKTGLGYTLKGVGDNSQM
                     IKSLGINIGSIKILGLMISNGLIALSGSLMAQFLGFSDVNMGIGTLVLGIASIIIGIT
                     LFKKFTFIKDTTAIIVGSFIYQFTIYFAMSLGMLSTDLKLITAIVIISFLATGNLNIS
                     LKKNKCKASTKNKSEKRGVVDVTN"
     gene            1500..2255
                     /locus_tag="PC471_003106"
     CDS             1500..2255
                     /locus_tag="PC471_003106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDF2790214.1"
                     /translation="MLQIKNLSKSFPNPYGEPNTIFENLSIDIEDGEFVSIIGSNGTG
                     KSTLLNIISGLIKESSGQVLLNKHNLSNLAEHRRTQIVSRVFQDPSLGTCPSMTVREN
                     LSLALNKGKLLNLRKCLRYKRDFLENLLEGVSLDLKKYLDVQVKFLSGGQRQSLSLIM
                     SCLTNPSVLLLDEHTAALDPKTSNEVIELTNKIVREKNITTLMVTHNLKHALHYGDRL
                     IMLHKGEVVLDVKGKEKEQLTVEEILEKFEYAV"
     gene            2386..3408
                     /locus_tag="PC471_003107"
     CDS             2386..3408
                     /locus_tag="PC471_003107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDF2790215.1"
                     /translation="MINKKRLASLILAGALSISMLTGCSQGGDSGNSKQESNSKGKEV
                     KKIGITQLVEHPALDATRTGFVKALEKNGFKDGENIDIDFQNAQNDMPTTQSIASKFA
                     SDKKDLIFAISTPSAQAAFNATKDIPILITAVSDPVAAGLVKTLEKPGTNVSGTSDFV
                     SVDKGLELLKIFAPKAKTIGVMYNTSEVNSKVQVDALKEYASKNGFKVVEKGITTSNE
                     VNQGISSLVGKIDVLYVPTDNLVASSMPIVSKIATENKIPVIAAESGPVEKGALACQG
                     INYEKLGYKTGEMAVKILNGESVSDMPVATSDDTDIIVNEDILKALGMEKPSNENISY
                     VKTKQE"
     gene            complement(3572..4129)
                     /locus_tag="PC471_003108"
     CDS             complement(3572..4129)
                     /locus_tag="PC471_003108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HDF2790216.1"
                     /translation="MTEKEKMLSGKGYYANDELLVKEREYCKKLTRLFNNTLEDEYEK
                     REDILRQLFGSVGKQINVEQNIRCDYGYNIHVGENFFANYDCIFLDVCKIEIGDNVML
                     APNVQIYTAYHPIDAQLRNSGIEYGSPIKIGDNVWIGGGVIITPGITIGDNVVIGAGS
                     VVTKDIPPNTVAVGNPCRVIKKIEE"
     gene            complement(4135..5211)
                     /locus_tag="PC471_003109"
     CDS             complement(4135..5211)
                     /locus_tag="PC471_003109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/molybdate ABC transporter ATP-binding
                     protein"
                     /protein_id="HDF2790217.1"
                     /translation="MSLYVDIEKDLSSFKLKVEIKQEKGTLGFLGESGSGKSMTLKCI
                     AGLEKPTRGKIVLNDRVLFDSEKKINLSTQDRKVGFLFQNYALFPHMTVSQNIELGLL
                     KLSKSEKKEIVARYLDILKLNGFEGRYPWQLSGGQQQRVALARALATSPDILLLDEPF
                     SALDHHLRSNMEKELMNMLKDYKGDILFVTHDIEEAYRVCDDIIVYNKGEGLPKRPKK
                     ELFESPKYLIEAKITGCKNISKLNRLDKNTIYATDWGCELTLNREIGDNIEYVGIREH
                     HIKVLDSNEDLNEKLCFELINIVENPFTYTIYVRKTDLSNECVPIQIELEKSKMRFKK
                     GDRIYLDFPQEYLFCFRYNYNKKE"
     gene            complement(5212..5895)
                     /gene="modB"
                     /locus_tag="PC471_003110"
     CDS             complement(5212..5895)
                     /gene="modB"
                     /locus_tag="PC471_003110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HDF2790218.1"
                     /translation="MGTDWSPLWISLKTSILSTIITFVIGIFVSYIMANYRGKWKGLI
                     DGLFTLPLILPPTVVGFFLLLLCGKNGFIGKFLLNFDRTLIFSWTATVISAVVVSFPM
                     MYRTSRSAFEQIDNNMLSAARTLGLNEWKVFFKIAVPLAWPGIIGGLVLSFARALGEF
                     GATLMIAGNIPGKTQTMPIAIFFAVEGGDMNKAMMWVMIIVAISIAMILLLNYWSEFQ
                     QKIIGKRCG"
     gene            complement(6044..6856)
                     /gene="modA"
                     /locus_tag="PC471_003111"
     CDS             complement(6044..6856)
                     /gene="modA"
                     /locus_tag="PC471_003111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HDF2790219.1"
                     /translation="MKKILGILGLVACLTLGTVGCNSNEDKKDDGKQEKTTKSSDSVE
                     LNISAAASLKEAMAKIEEEYKKVDSNVKLTVNYGASGSLQQQIEQGAPCDLFISAGQK
                     QMKALDEEKLLVSDTMKDLVKNDLVLISSADSSVSGMKDLTTDKVKKIAVGEAESVPA
                     GKYADEVLTNLNLKDKLKDKLVFAKDVKEVLAWVQSGNADVGFVYFSDTVNNDKIKVV
                     EKTDEKTHSPITYPVSVIKASKNVDAAKKFEEFLLSESGQKIFEEFGYKKVE"
     gene            7049..7978
                     /locus_tag="PC471_003112"
     CDS             7049..7978
                     /locus_tag="PC471_003112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2790220.1"
                     /translation="MDSLSSLTPVEVAELLKITKNTVYELIKRGELPSYKVGKKIRVD
                     TKDVEEYINSQKTGKVKDTHFNNKNIDINNNFKIIISGQDVILDILARSVEKKLDGIS
                     TFRSYIGSYNALYELYNGRVSIASSHLWDFETDEYNSTFVKKLLPGIPCVLINLAYRM
                     QGFYVAKGNPKEITTWEDLTKPSITIVNREKGSGTRVLLDGKLRLLNFNGKHIKGYDN
                     EELSHLGVASTVSRGIADVGLGNEKAALQVNNIDFIPLHKERYDLVIKKEDLQNPVYQ
                     TIINIINSPEFKAELQGLGGYDLTDTGKTIAKV"
     gene            complement(8139..8588)
                     /locus_tag="PC471_003113"
     CDS             complement(8139..8588)
                     /locus_tag="PC471_003113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2790221.1"
                     /translation="MKNKNNKDKNMLNIKVLRVLGVLLILFDYTGDTLNFYNFFAKPL
                     VSGNLYKTTGTDCIVFLLFIVECVYLVGRYILKVKINWSEIIENEKGNFINNLLVEKI
                     QEESNIVYNLLNSYVLPNIYKKARGLMFKTYHMLIYIKEKYYYVNIY"
     gene            complement(8791..9771)
                     /locus_tag="PC471_003114"
     CDS             complement(8791..9771)
                     /locus_tag="PC471_003114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDF2790222.1"
                     /translation="MKNSFKNCISLRNKFIIISIVFLLILLIVSYIFGKLVYNSSVGL
                     EQLIKNEDIVKIFSERKDRPLEKLKNYKTSELMIQSSNNYKLESLFITSNIKTRDTMI
                     LVHGIGSSYYEMLKVAYRYLDKGYNVLVYNQRNTGNSGGDNYTFGLYERYDLDSLVKF
                     VKNKFPEGRLGVHGFSMGAGTAAMHSEINSKDDKVDFYILDSPYSEMKDAIRMGVLEK
                     RIPDILINYVVTCGDLYNKFKSGFWYSDVKPYESVEKSNVPILFIHGTKDTVCNYQNS
                     KKMYDLVKHDKKDLWLIEGIGHVDGFEHDSTVYFNKIFKFIDSNVLSDKS"
BASE COUNT         3377 a         1560 c         1208 g         3652 t
ORIGIN      
        1 tatgttgatt taaaacctat ttacactaat tatctaaata tctctttatt tttataatct
       61 tttataactt atttatattt ttcattttta taaaaaaaca ataattaaga atataactat
      121 attctacatg taaaaatttt tttatttaaa ctcaattttc tgataatttg ataatttttc
      181 aaaatttata gttgactttt attttttact gtattaaact aattactata caaaacagaa
      241 aacaaaattt tcaaaattat taaattaaaa ggagaggtaa atatgaagac tttattaaac
      301 aaacaagtac gattttttag tagccaattt agttagggtt tgtacctaag ttaagacaaa
      361 ttgtcataac atagatacaa gccccttagg attatgattt ttaaggattg tatctatgtt
      421 ggctatagta attgttgtta aagtttttat tttatcatca taatatagag ccacatggat
      481 tatactgtgt ggcttattcg aatttttgcc atacagttat atatactgtg tggttttttt
      541 atatcaaaat cattttttaa aaccaaattt taaaaaccat ttaaaatatt ttcaaagggg
      601 tgttttattt atgtcaggta ttatatctgt aatgacgcaa agtttaattt tatcgattat
      661 ggcactgggg gtttatataa cttacaaaat cttagatttt ccagatatgt ctgctgatgg
      721 tagctataca atgggtgctt ctattgtagc attttctctt actaatggta tttctcctgt
      781 agtagcaact ttaatggcta tcttatgtgg ttgtactgct ggtcttgtta cagggattct
      841 tcacattaag tttaagatat ctaatttact ttctggtatt ttagttatgg gaatgctata
      901 ctctatcaat ttaagaataa tgggaaaatc aaatattcct ctatttagct ttaaacactt
      961 attcaatggg gaaatatctc ctatagtttt agccttagct tttgtattca tctgtaaagt
     1021 ccttttagat ttattcctaa aaacaggtct tggatacact ttaaaaggtg ttggtgataa
     1081 ctctcaaatg attaaatccc taggtataaa tatcggttct ataaaaatac taggacttat
     1141 gatttcaaac ggacttatag ctttatctgg tagtcttatg gctcagttct tgggattctc
     1201 agatgtaaat atgggtatag gaactttagt tcttggaatt gcctctatca taattggtat
     1261 tacactattt aagaaattta cttttatcaa agatactact gcaataattg ttggctcttt
     1321 tatataccaa ttcactatat actttgcaat gagtttagga atgttatcaa ctgacttaaa
     1381 actaataact gccatagtta taatatcttt cctagctact ggtaacttaa atatttcact
     1441 aaaaaaaaac aaatgcaaag ctagtaccaa aaataaatca gaaaaaagag gtgttgtaga
     1501 tgttacaaat taaaaatctt tcaaagagtt tccctaatcc atatggtgaa cctaatacta
     1561 tctttgaaaa tttatcaatc gatatagaag atggtgaatt tgtaagtatc ataggtagta
     1621 atggaactgg taaatctact ttattaaata taatatccgg gcttataaaa gaatcttctg
     1681 gtcaagtatt attaaataaa cataacttgt ctaatttagc tgaacacaga agaactcaaa
     1741 tagtaagtag agtttttcaa gacccaagtc ttggaacttg cccttctatg actgttagag
     1801 aaaacttgtc tttagctctt aacaaaggaa aactattaaa tctaagaaaa tgtcttcgtt
     1861 acaaaagaga tttcttagaa aatttattgg aaggtgtatc tcttgattta aaaaaatacc
     1921 ttgatgttca agtaaaattt ttatcaggag gtcaaagaca atcattatca ctaattatgt
     1981 cgtgcttaac aaatccatca gttttattac ttgatgaaca tacagcagca cttgacccaa
     2041 aaacatcaaa tgaagtaatt gaattgacta ataagattgt aagggaaaaa aatataacta
     2101 cacttatggt tactcacaac ttaaaacacg ctcttcatta tggtgataga ttgataatgc
     2161 ttcataaggg tgaagttgta ttagatgtaa aaggtaaaga aaaagaacaa ttaacagttg
     2221 aagaaatctt agaaaaattt gaatatgcag tttaaaataa tatatctatt gtattaaact
     2281 cggctttaaa ataaagttaa aattacaata attatggtta ctttgtaata attcatatga
     2341 atatatgaac atatataaca ataaattttt gagggggata taaaaatgat taataaaaaa
     2401 agattggcaa gtttgatttt agctggtgct cttagtatat ctatgcttac agggtgttca
     2461 caaggaggag actctggtaa ttcaaaacaa gaaagtaatt ctaaaggtaa agaagttaaa
     2521 aaaattggta taactcaact ggttgaacat ccagcattag atgccactag gacaggtttt
     2581 gtaaaagcat tagaaaagaa cggatttaaa gatggagaaa atattgatat agacttccaa
     2641 aatgctcaaa atgacatgcc tactacacaa agtattgcga gtaaatttgc atctgataag
     2701 aaggacttaa tatttgctat atctactcca tcagctcagg cagcatttaa tgctactaaa
     2761 gatataccta tacttattac tgctgtgagt gaccctgttg ctgctggatt agttaaaact
     2821 cttgaaaagc caggcacaaa tgtatctgga acatcagatt ttgtttctgt agacaaaggt
     2881 ttagaacttc ttaaaatatt tgctcctaag gcaaaaacta taggagttat gtacaatact
     2941 agtgaagtaa attccaaagt tcaagttgat gctttaaaag aatatgcatc taaaaatgga
     3001 tttaaagttg ttgaaaaagg tataactact tcaaatgaag ttaaccaagg tatttctagt
     3061 ttagttggca aaatagacgt attatatgtc cctacagata acttagtggc ttcttctatg
     3121 cctatagtct ctaaaatagc tactgagaat aaaatacctg ttatagcagc tgaatctggt
     3181 ccagttgaaa aaggtgctct tgcttgtcaa ggtatcaact acgaaaaact aggttacaaa
     3241 acaggtgaaa tggcagttaa aattttaaat ggcgaatcag tatctgatat gcctgtggct
     3301 acatcagatg atacagatat cattgttaat gaagatatat taaaagcttt aggtatggaa
     3361 aaaccttcta atgaaaatat ttcttatgta aagactaaac aagaatagaa agatattttc
     3421 tatatacgat taatatgcct gtttctataa attaatttat agaaacaggc atatattttt
     3481 attttaatgt attatctatt taaagattat atgtaaatgc tcttaatttg tttgtataaa
     3541 ttttacttgt ataaacttta tacaatcata tttattcttc tattttcttt attactctac
     3601 atggatttcc aacagctaca gtatttggtg gtatatcttt tgtgactaca cttcctgcac
     3661 ctattactac attatcacct atagtaattc ctggtgttat gattactccc ccacctatcc
     3721 atacattatc acctattttt ataggagaac catactctat gcctgaattt ctaagttgtg
     3781 catcaattgg atgataggct gtatagattt gaacgtttgg agctaacatt acattgtcac
     3841 ctatttcaat tttacataca tctaaaaaaa tacaatcata atttgcaaag aaattttcgc
     3901 ctacatgtat gttatatcca taatcacacc taatattttg ttctacattt atttgcttgc
     3961 caactgaacc aaatagttgt cttaaaatat cttctctttt ttcatattca tcctctaaag
     4021 tattattaaa caatctagtt aattttttac aatactctct ttcttttact aacaattcat
     4081 catttgcata gtatcctttc ccagacaaca ttttttcttt ttcagtcata tagtttactc
     4141 ctttttgtta taattgtatc taaagcaaaa taaatattcc tgtggaaaat ctaaatatat
     4201 tctgtcacct ttcttaaatc tcattttact tttttccaat tctatttgaa taggtacaca
     4261 ttcgttagat aaatcagttt ttctaacata tatagtatat gtaaaaggat tttctacaat
     4321 gttaatcaat tcgaaacata acttttcatt taaatcttca ttagaatcca agaccttaat
     4381 atgatgctcc ctgattccca catattctat attatcacct atttctctat ttaatgtcaa
     4441 ctcgcatccc cagtctgtag cataaatagt atttttatcc agtctattta atttggatat
     4501 atttttacac cctgttatct tagcttctat aaggtacttt ggagattcaa agagctcttt
     4561 tttaggtctc tttggaagac cttctccttt gttgtacact ataatatcat cacaaactcg
     4621 gtaagcttct tcaatatcat gagtaacaaa tagtatatct cccttgtaat cttttaacat
     4681 attcataagt tctttttcca tattgcttct taaatgatgg tccaatgctg agaaaggttc
     4741 atctaaaagt aatatatctg gagatgttgc caatgctcta gctagtgcta ccctttgttg
     4801 ttgaccacca gataattgcc aagggtatct cccctcaaac ccatttaatt taagtatatc
     4861 taagtacctt gctactattt cctttttttc actcttactt agttttaaaa gcccaagttc
     4921 tatattttga cttacagtca tatgtggaaa caaagcatag ttttgaaata aaaatcctac
     4981 ctttctatct tgtgttgata aatttatctt cttttcagaa tcaaatagaa ctctatcatt
     5041 taatacaatt ttacctcttg ttggtttctc aagccctgct atacatttta atgtcatact
     5101 ttttccagaa ccagattctc ccaaaaaccc cagagtacct ttttcttgtt taatttctac
     5161 ctttagttta aaagaggata aatctttttc aatatccacg tataagctca actatccaca
     5221 cctctttcct ataattttct gctgaaattc cgaccaataa tttagtaata aaatcattgc
     5281 tatagatatt gctacaataa tcataaccca catcatagct ttattcatat ctccgccttc
     5341 aacagcaaaa aatatagcta ttggcatagt ttgagttttt ccaggtatat ttccagctat
     5401 cataagtgta gcaccaaatt ctccaagagc tctggcaaac gacagcacca atccaccaat
     5461 tattccaggc catgctaatg gaactgcaat cttaaaaaat actttccatt cattaagacc
     5521 taatgttcta gcagctgaca acatattatt atcaatctgt tcaaaagctg accttgaagt
     5581 tctatacatc ataggaaaag acaccactac tgctgaaatt acagttgctg tccaactaaa
     5641 tattaaagtc ctatcgaaat ttaataaaaa ctttcctata aatccattct tcccacatag
     5701 taataataaa aagaaaccta caactgttgg tggcaaaatt agtggaagtg taaatagtcc
     5761 atctataaga cccttccact tacctctata gttagccata atatatgaca caaatattcc
     5821 tattacaaaa gttattattg ttgacaaaat agatgtctta agtgatatcc ataagggtga
     5881 ccaatctgtt cccatctttc cctccttaat tttaaatgga aatagtgaaa ctaataactc
     5941 tattaatttc actatttcca ttttattata atacacaatt attttcaatt ttcatttatt
     6001 tatatataat ttatacttaa ttacacataa tttatttcta agtttattct acttttttat
     6061 atccaaattc ttcaaaaatc ttttgtcctg actcacttaa taaaaattct tcaaatttct
     6121 tagcagcatc tacgttttta ctagctttaa ttactgaaac tggatatgta ataggcgaat
     6181 gagttttttc gtctgttttt tcaactactt ttatcttatc attattgaca gtatcactga
     6241 agtaaacgaa tccaacatca gcatttcctg attgcaccca tgctaaaact tcttttacat
     6301 cttttgcaaa tactagctta tcttttaact tatcttttaa atttaaatta gttaacacct
     6361 catctgcata cttaccagct ggaacacttt cagcttcacc aacagcaatt ttcttcactt
     6421 tatctgtagt caaatctttc atacctgata cactactgtc agcagaagaa ataagaacta
     6481 aatcattttt tactaaatct ttcatagtat cagaaactaa taacttctct tcatccaaag
     6541 ctttcatttg tttttgacct gctgaaataa ataaatcaca aggtgctcct tgttctattt
     6601 gttgttgtaa tgagcctgat gctccatagt taactgtaag ttttacattt gaatcaactt
     6661 ttttatattc ttcttcaatc tttgccattg cttcttttaa acttgcagca gccgatatat
     6721 ttaactctac tgaatcacta cttttagttg ttttttcttg ttttccatca tcttttttgt
     6781 cttcattact attgcaccca actgtcccca aagttaaaca tgcaactaat cctaatattc
     6841 ccaatatttt tttcataaca atctcccttt ttgtaaattt gatacacatt ttaacatgtt
     6901 ttattatgtt ttatcatgtt ttgttatata tagtttaatt atatatacat gataataaat
     6961 tttcaagtaa tttatttaca ctacttttaa atattttgct acattttcct ttttttaatg
     7021 ttaaaataac cttatatgga ggtggattat ggactcatta tcatctttga cacctgtcga
     7081 ggtagctgaa ttattaaaga taacaaaaaa tactgtatat gaattaatca aaagaggtga
     7141 acttccttct tataaagtag ggaaaaaaat aagagttgat actaaagatg ttgaagaata
     7201 tatcaacagc caaaaaactg gtaaagtaaa ggatacacat ttcaacaata aaaatataga
     7261 tataaataat aatttcaaaa ttattatttc tggtcaagat gtaatattag acattttagc
     7321 taggtctgtt gaaaaaaaat tggatggaat aagtacattt cgctcttata taggtagtta
     7381 taatgctttg tatgagttat ataatggcag agtttctata gcatcatccc atctatggga
     7441 tttcgaaacg gatgaatata atagcacttt tgttaaaaag ctattgccag gaataccatg
     7501 tgtacttata aatctagctt atagaatgca aggtttctat gttgctaaag gaaatcctaa
     7561 agagataaca acctgggagg atttaacaaa accaagtata accattgtaa atagggaaaa
     7621 aggttctgga actagggttt tactagatgg taaacttaga cttctaaact ttaatggtaa
     7681 acatataaaa ggttatgata atgaagaatt atcacatctt ggagttgcaa gtactgtttc
     7741 tcgtggaata gcagatgttg gtttgggaaa tgaaaaagct gctttacaag taaataatat
     7801 cgactttatt cctcttcaca aagagcgcta tgatttagtt attaaaaaag aagacttaca
     7861 aaatccagtt taccaaacta taattaacat aataaactct cctgagttta aagctgaact
     7921 tcaaggtctt ggcggatatg atttaactga tactggaaaa actatagcaa aagtttaaga
     7981 ttaaaatcgc aatctatttt ataagattta taatttttca caaaatttat aacttaaaca
     8041 cgaaataaat tgcgatttta gtgtctcaaa aaatttatgt ctttcatttt atcatccaat
     8101 acaaaatata atataattaa ttgtgcaatc atattatatt aatatatatt tacataatag
     8161 tatttttctt taatgtagat taacatatga tatgttttaa acatcaatcc ccttgctttc
     8221 ttatatatat ttggcaacac atatgagttt aataaattat atacaatgtt gctttcttct
     8281 tgtatttttt caaccaacaa gttatttata aaattacctt tctcattttc aataatctca
     8341 ctccaattta tcttaacttt taatatatat cttcccacta ggtatacaca ctctacaata
     8401 aataataaaa atacaatgca gtcagtacct gttgtcttat ataaattacc cgatactaat
     8461 ggctttgcga agaaattata aaaattcaaa gtatcacccg tataatcaaa aagtattaat
     8521 agcactccta gaactctcaa tactttaata ttcaacatgt ttttatcttt attatttttg
     8581 tttttcatat atactacctc tcttttcttt agatttgatt gtaatctaat actaactctt
     8641 ttatttgagg tataaatgaa tttaattggt agttattttc atttttacta gaaattaatt
     8701 cacattacta aacaaaactt atatttaaac ataaaaatag cctatctatt ttttaataaa
     8761 tacaaaaata aataagctac taatattcaa ttaactttta tcacttaaaa cattactatc
     8821 tataaattta aatattttat tgaagtatac tgtactatca tgttcaaatc catcaacatg
     8881 accaatacct tctataagcc ataggtcttt tttatcatgt ttaactaaat catacatttt
     8941 cttgctattt tgataattac aaacagtatc ttttgttcca tgtataaaca gtatgggtac
     9001 attagatttc tcaactgact catatggttt tacatcactg taccaaaatc cagatttaaa
     9061 cttattatat aaatctccac aagtcaccac ataattgatt aatatatctg gtattcgttt
     9121 ttctagtact cccattctaa ttgcatcttt catttcagaa taaggagaat ccagtatata
     9181 aaaatcgacc ttatcatctt ttgaatttat ctctgaatgc atagcagctg tccctgcccc
     9241 catggaaaat ccatgcactc ctaatcttcc ttcaggaaat ttatttttta caaatttaac
     9301 tagactatct aaatcatacc tttcataaag accaaatgtg taattatctc caccactatt
     9361 accagtattt ctttgattat aaactaatac gttataacct ttatctaaat atctatatgc
     9421 tacctttaac atttcatagt aactacttcc aattccatga acaagtatca tagtatctct
     9481 tgtctttata tttgaagtta taaaaagact ttcaagcttg taattattag aactttgtat
     9541 cattaattca gatgtcttgt agttttttaa tttctccagt ggtctatctt ttctctcaga
     9601 aaatattttt actatatctt cgttttttat taactgctct aaaccaactg aactattata
     9661 cactaatttg ccaaatatat aagatacaat taataggatt aatagaaaca caattgatat
     9721 aattataaat ttatttctta aactaataca atttttaaaa ctatttttca tatttattta
     9781 ccatacaatt ttcctat
//
LOCUS       DAMJYR010000130         9747 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.135, whole genome shotgun sequence.
ACCESSION   DAMJYR010000130 DAMJYR010000000
VERSION     DAMJYR010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9747)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9747)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9747
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.135"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            60..734
                     /locus_tag="PC471_003115"
     CDS             60..734
                     /locus_tag="PC471_003115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2790223.1"
                     /translation="MFKIMVVEDDVSLKNIIAKCLTKWGHDVHQIENLENIIEEFKNY
                     NPELVLLDINLPFYDGFHWCNEIRKISKVPIIFISSRNSNMDVIMGVNLGADDYIQKP
                     FSVDVLVAKVNALLRRTYNFVDNNSNQIIHNGVTLDLSTATINYEDNTIELTKNEIKI
                     LHELMKYKGQIVSRNKLMKKLWDNDWFVDDNTLTVNVNRIRSKLNEIGLEDFIETKRG
                     LGYIIS"
     gene            758..1762
                     /locus_tag="PC471_003116"
     CDS             758..1762
                     /locus_tag="PC471_003116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF2790224.1"
                     /translation="MSFKDFLKDKLGFLVYNFTFLIFTVSVIIFSPVDVFLTDTILYI
                     LVVNVVFLFLYLFISYIKKNKFLDKIKNDTFKINMNDIELARCKNEEKIYLNIIKNHQ
                     YQCNNIINNNIEKFEENKEILNMWVHDVKIPIAIIKLILEQNENLIDEKISDDIDSEI
                     FRIENSVEKILYLSRLEDFHKDFLIQEVNIEKIIRNIIKKYSKYFISKKIILDLNNLN
                     FKVLSDEKWLYFIFEQLLSNSLKYSSLGDNISIYCKRTKNYLQLRVEDTGCGIKKEDL
                     NRIFNKGFTGNNGRNNSKSTGLGLYLVKELCDKLGHKIDVDSEYNQYTKFTITFKCDL
                     "
     gene            1874..3103
                     /locus_tag="PC471_003117"
     CDS             1874..3103
                     /locus_tag="PC471_003117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HDF2790225.1"
                     /translation="MSFLKKFKNKRFRNNSFKSNNFRNSSFGSSGFKSKNKGFKNNPF
                     KNKRFKIYAGIAVIILLILGVLAYVDSKNTELQANENFIETYTIPENEKIFINGMVVP
                     KQTKDFNISGDYELSDVNVTNGQKVNQGDLLFTAKNPTIIAEIDSLKSQLSQYKKQKI
                     SLSDIAENKDAIASINAQITALSSQIASLDKKAYDRVTAPFDGTVYLNDQTGNPDQPV
                     SFMTIQGLEFYMKGQASEQDLPKMKIDQMVNILVFSTDQKLTGRISFISDKPSTPNTE
                     MGAQQNTLSYYDINIAFDNQEGLVNGFHLQASLEVANSSFKIPASCVLKDNKHSYVFK
                     DLDGILKKQIVDVASQNDDFAVVRGGLEQGDIIIKHPTKEMKEGDPVQGDGVTAGSNN
                     GDTTPNKKVEEVTMDVN"
     gene            3111..3785
                     /locus_tag="PC471_003118"
     CDS             3111..3785
                     /locus_tag="PC471_003118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2790226.1"
                     /translation="MLIKLENIQKYYKVGKDELHVLKSLNLEIESGEFVMIMGKSGSG
                     KTTLLNILGFLDVFDEGRYIFDGTDVTNLSENERSVFRNINIGFVFQQFNLIETLNVY
                     QNVELPLIYNKALKKSNREEIVKDKLSSVGLLDKLKQKPLQLSGGQQQRVAIARCLAN
                     DPQIIFADEPTGALDSETSREIMELLTRLNKQGKTIIMVTHDQDLTKYATKVIRLKDG
                     VFTSEV"
     gene            3788..5020
                     /locus_tag="PC471_003119"
     CDS             3788..5020
                     /locus_tag="PC471_003119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2790227.1"
                     /translation="MSLLKNSLANLKGHKLRVFVALLWIIIGITSVILVSSIGNGFQK
                     EIKKSVNNVNPNKTTISFESADNTGLTDDMSIFLKPFNAKDLEELSFVEGVERIAPSR
                     DGFNLDSVYSSQASFDKKTTYVDVGPVKKDSKINLICGRDFSLDDEKRKVILLTLQST
                     SEIFENPEDALGHGININGTIFEIIGVLDDSQQNQAGGFFGGYQDMQFTTSLVPKKAF
                     DTLMSQNSYSNEIYQLDLVSSKGYNVNEVANNVIAKLYEMHPGINGSYTTPDPTEQTA
                     YLESINSNVNKYVSIITVVAMFVGGIGVMNIMYVSVMERQREIGIRRAIGAKPRSILF
                     QFLVEAVFITVCGGILGTIVGFTATNYVSKYIGFEAIPSLNSLFYAIVATILTGVVFG
                     LIPAFKASKLDPIKAIYK"
     gene            complement(5306..6571)
                     /locus_tag="PC471_003120"
     CDS             complement(5306..6571)
                     /locus_tag="PC471_003120"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HDF2790228.1"
                     /translation="MILIKNGRVIDPKSQRDENIDLIIKENKIYKIGKFDESDEYEKI
                     IDASGNVVAPGLVDVHVHFRDPGFTYKEDIESGAKSAARGGFTTVICMANTNPIVDNE
                     DTFNYVKEKSKNACINVLQAAAITKGFEGKELVDMEALKKAGVPGFTDDGLPLMDSNL
                     IMEAMIKAKELDVPLSFHEEDPSLVGNPGVNAGKVASELGLKGASSVAEDVMVARDCM
                     LALKTGAKVDIQHISSGVSVDMVRFAKYLGANVVAEASPHHFTLTEEDVLEFGTNAKM
                     NPPLRSKWDRDKIIEGLNDGTIEIIATDHAPHSKEEKDREFIKAPSGIIGLETSLALG
                     ITNLVHKNHLSMMQLIEKMSINPAKLYNLNIGFIEEGAVADIVIFNPEEEWTVESFVS
                     KADNSPFKGKSLYGKVNYTICNGEIVYNA"
     gene            complement(6624..7772)
                     /locus_tag="PC471_003121"
     CDS             complement(6624..7772)
                     /locus_tag="PC471_003121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HDF2790229.1"
                     /translation="MKSVSVRSCKTYNYEEVKLSIEKNLEDIGGIDKFVKKGSKVLIK
                     PNLLMKKKPEEATTTHPMVVKVVCEKLLELNCDITIADSPGGPYNQSSLKSIYKASGI
                     EEVANELGIKLNYDVSDVKIHSEKAHVLKYMDIIKPIVDSDYIINLCKLKTHIMATFT
                     GGTKNLYGCIAGLKKAEIHYRFPTEELFCENVLLDICDYIKPTLTIMDGIVGMEGDGP
                     SAGTTREIGVLLASQNPYAIDVVACKIISLLPNRVATIRGAIKRGYIQEDFSDIEILG
                     EDIKDLIIKNYKIPNVSKDLRLFSVKLPKFLNEPISKLITPKPVVRFQDCIKCGKCKE
                     ACPASTIEIGTKGAIIDLSKCIRCYCCHELCPKKAIDVKRNFVFKYVK"
     gene            complement(7863..8408)
                     /locus_tag="PC471_003122"
     CDS             complement(7863..8408)
                     /locus_tag="PC471_003122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH peroxidase"
                     /protein_id="HDF2790230.1"
                     /translation="MKKFVCTVCGYIHEGDAAPAQCPVCKVGADKFEEMKGEMVWADE
                     HRIGVAQGVDAEIIEGLRANFTGECTEVGMYLAMSRQADREGYPEVAEAYKRIAFEEA
                     EHAAKFAELLGEVVVADTKENLRVRVDAEYGATDGKLKLAKRAKELGLDAIHDTVHEM
                     CKDEARHGKAFLGLLNRHFGK"
     gene            complement(8631..9575)
                     /locus_tag="PC471_003123"
     CDS             complement(8631..9575)
                     /locus_tag="PC471_003123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HDF2790231.1"
                     /translation="MQKNKKEGALLKKYFGEIGLIFIAIIWGSGFVATQFALDGGLTP
                     LQIITLRFFLAAIIMNLLFFKQIRANMGKKLLKAGGILGIFLFLAFTVQTIGLMYTTP
                     SKNAFITAANVVIVPFIGFILYRRKLDKIGIISSLVALIGIGILSLEADFSINFGDFL
                     TLICSFGFAFHIFFTSEFAKDNNPMALTAIQFTVAFLMSVVVQTFAGQLKMEAELSGY
                     MGTMYLAVFSTTIGFLFQTICQKRVDGTRTAIILSTEAVFGTIFSIIILKELITAKLV
                     IGSILIFVAIITAETKLSFLKSKKVKLKDSEESSLESI"
BASE COUNT         3473 a         1509 c         1302 g         3463 t
ORIGIN      
        1 taatttatca agtagaatat agttatgtta atataactat tttgaattag gaggtaagaa
       61 tgtttaaaat aatggtagtt gaagatgacg tctcattgaa aaatatcata gcaaagtgtc
      121 ttactaaatg gggacatgat gttcatcaaa tagaaaatct tgaaaatata atagaagagt
      181 tcaaaaacta taacccagaa ttagttttat tggacataaa ccttccattt tatgatggtt
      241 ttcattggtg taatgaaata agaaaaattt caaaagttcc tataattttt atttcttcaa
      301 gaaattctaa tatggatgta ataatgggtg taaatttagg tgcagatgat tatatacaaa
      361 aacctttttc tgtagatgtc ttagtagcta aagttaatgc ccttttaaga agaacttata
      421 attttgttga taataactca aatcagatta ttcacaatgg tgttacttta gatttatcaa
      481 ctgcaacaat aaactatgaa gataatacta ttgagctaac aaaaaatgaa atcaaaattc
      541 ttcatgagct tatgaaatat aaggggcaga tagtaagtag gaataaactc atgaaaaaat
      601 tatgggataa tgattggttt gtagatgata atacattaac tgtcaatgta aatagaatac
      661 gctcaaagtt aaatgaaatt ggtttagaag attttataga aacaaaaaga ggattgggtt
      721 atataatcag ttaaaacaat tctaagtagg tgaatttatg agctttaaag attttctcaa
      781 ggataaacta ggttttttag tatacaattt tactttctta atttttacag tatcagttat
      841 tatattttct cctgtagatg tttttctgac tgatactatt ttatatatat tagttgtaaa
      901 tgttgtgttt ttatttttat atctatttat tagctacatc aagaagaaca aatttttaga
      961 taagataaaa aatgatacat tcaaaattaa tatgaatgat atagaattag ctagatgtaa
     1021 aaatgaagaa aaaatatact tgaacattat taaaaatcat caatatcaat gtaataacat
     1081 aataaataat aatatcgaga aatttgaaga aaacaaagag attttaaata tgtgggtaca
     1141 tgatgtaaag atacctatag ctataataaa actcattctt gaacaaaatg aaaatttaat
     1201 tgatgaaaaa atttcagatg acatagatag tgaaatattt agaattgaaa actccgttga
     1261 aaaaatttta tatttatcaa gactcgaaga ttttcataag gattttttaa ttcaagaggt
     1321 aaatattgaa aaaatcatta gaaatattat aaaaaaatac tcaaaatatt ttatttcaaa
     1381 aaaaattatt ttagacttaa ataatttgaa ttttaaggtt ttatctgatg aaaaatggct
     1441 atacttcata ttcgagcaat tattgagtaa ttccctaaaa tactcctcat taggagataa
     1501 tatatctatc tattgcaaaa gaaccaaaaa ttatttacaa ttaagagtgg aagatactgg
     1561 ttgtggtata aaaaaggaag atttaaatcg aatattcaat aagggattta caggtaataa
     1621 tggaagaaac aattctaaat ctacaggtct tgggctttat ttagtaaaag aactttgtga
     1681 taagcttggt cataaaattg atgtagattc agagtacaac caatatacaa aatttactat
     1741 tacattcaaa tgtgatttat agtatcttat tattgatagt ttattttcat taacttatat
     1801 tcaacttctt atagtaattc tatatgtaca tcccagtata taattaagta gtttttataa
     1861 aaaggaggaa gacatgtctt ttctaaaaaa atttaaaaat aaaagattta gaaataacag
     1921 ttttaaaagt aacaacttta gaaatagcag ttttggaagt agtggcttca agtctaaaaa
     1981 taaaggtttt aaaaacaatc cctttaaaaa taagagattc aaaatatatg caggaatagc
     2041 agtaatcata cttttaattc taggagttct tgcttatgtt gattcgaaaa acactgaatt
     2101 acaagctaat gaaaatttta tcgaaacata tactattcct gaaaatgaaa aaatatttat
     2161 aaatgggatg gttgttccta agcaaactaa ggacttcaat atatctggag attatgagtt
     2221 aagtgatgtt aatgttacaa atggtcaaaa agtaaatcaa ggagaccttc tttttactgc
     2281 taaaaatcca actataatag cagaaataga tagtttaaaa tcacaactta gtcaatacaa
     2341 aaaacaaaaa atatcacttt ctgatattgc tgaaaataaa gatgctattg cttctataaa
     2401 tgcacagata actgctttaa gctcccagat agcttctctg gataaaaaag cttatgacag
     2461 agttactgct ccttttgatg gcacagtata cttgaatgac caaactggaa atccagacca
     2521 accagtttca tttatgacta ttcaaggtct agagttttat atgaaaggac aagcaagtga
     2581 acaagattta cctaaaatga agattgacca aatggtcaat atactagttt tttcaactga
     2641 ccaaaagctt actggtagaa taagttttat atctgataaa ccatctactc caaatactga
     2701 aatgggagct cagcagaata cactatctta ttatgatata aacatagcat ttgataatca
     2761 agaaggatta gtaaatggat tccatcttca agcatcactt gaagtagcta attcaagctt
     2821 taaaatacca gcatcttgtg tacttaaaga caacaaacac tcatatgtat ttaaggattt
     2881 agatggtata ttgaaaaagc aaattgtaga tgtagcaagt caaaatgatg attttgctgt
     2941 agttagaggc ggtcttgaac aaggcgatat aataataaaa caccctacta aagaaatgaa
     3001 agaaggagac ccagttcaag gcgatggcgt aactgctggt tctaataatg gagatactac
     3061 tccaaacaaa aaagtagaag aagttactat ggatgtgaat taatttacat atgttaatta
     3121 aattagaaaa tattcaaaaa tattacaaag tagggaaaga tgaattacat gttttaaaat
     3181 ccttaaattt agaaattgaa tctggagaat ttgtcatgat aatgggtaag tctggtagtg
     3241 gtaaaactac tttacttaat atcttaggtt ttctagatgt atttgatgaa ggaagatata
     3301 tatttgatgg aacagatgta actaatttga gtgaaaatga acgttctgtt tttagaaata
     3361 ttaatattgg atttgttttc caacaattta atttaataga aactctaaat gtatatcaaa
     3421 atgtagagtt gcctctaatt tacaataaag cactaaaaaa atctaataga gaagaaattg
     3481 ttaaagataa attaagttct gttgggcttt tggacaagct taagcagaaa ccacttcaat
     3541 tatctggtgg tcaacaacag cgtgtagcca ttgcacgttg cctagcaaat gaccctcaga
     3601 taatctttgc tgacgaaccg actggggcat tagatagtga aactagcaga gaaattatgg
     3661 agcttttaac aaggctcaac aaacaaggta agactataat aatggttaca catgatcaag
     3721 atttaactaa gtatgcaact aaggttatac gacttaaaga tggtgtgttt acttcggagg
     3781 tgtaatcatg agtttattaa aaaattcttt ggccaattta aaaggtcaca agcttcgtgt
     3841 atttgtagct ttactctgga ttataatagg aataacttca gtaatacttg ttagttctat
     3901 aggtaatgga tttcaaaaag aaattaagaa atctgtaaac aatgtaaacc ctaataaaac
     3961 tactatttcg tttgaatcag ccgataacac aggtcttact gatgatatga gtattttttt
     4021 aaaaccattt aatgcaaaag atttagaaga attatcattt gttgaaggag tagaaagaat
     4081 agctccttca agagatgggt ttaaccttga ttcagtttac tcttcacaag catcttttga
     4141 taaaaaaact acgtatgttg atgttggacc agtaaaaaaa gattctaaaa taaatttaat
     4201 ttgtggtaga gatttttctt tagatgatga aaaaagaaaa gtaattttac ttacattaca
     4261 aagtacaagt gaaatttttg aaaatccaga agatgcctta ggtcatggta tcaacataaa
     4321 tggaactata tttgaaataa taggggtatt agatgattct cagcaaaacc aagctggagg
     4381 attctttgga ggttaccaag atatgcaatt tactacttcc ctagtaccta aaaaggcttt
     4441 tgacacttta atgagtcaaa attcttattc aaatgaaatt taccagttag acttagtttc
     4501 ttctaaaggc tataatgtaa atgaagttgc aaacaatgtt atagctaaat tatatgagat
     4561 gcaccctggt atcaatggtt cttatactac accagaccca actgaacaga cagcttatct
     4621 tgaaagcatt aattccaatg taaacaaata tgtatctata ataactgtag ttgcaatgtt
     4681 tgttggtggt attggtgtaa tgaatataat gtatgtatct gttatggaaa gacaaagaga
     4741 aattggtata agacgtgcta ttggagcaaa acctaggtct atattatttc aatttttagt
     4801 tgaagctgtt tttataacag tatgtggtgg aattttagga actatagttg gatttactgc
     4861 tactaactat gtttctaaat atataggttt tgaagctata cctagcttaa atagcttatt
     4921 ttatgcaatt gtagctacta tattgactgg agttgtattt ggtctgattc cagcctttaa
     4981 agcctctaaa ctagacccta taaaggcaat ttataagtag aaattgtata aagattttcg
     5041 atttttagat tagtttatat aaaacatact taaatatata acgcactatt atatatgata
     5101 gattaggaac ttaatctttc caaatataat agtgcgttct ttcataaaaa ggattctctt
     5161 ccttactata tctaaattac caagatgtta tgttctaatt gttcaaacta atacttccaa
     5221 ttttgattaa tcaatctcat tctcctaata tacaatcttt tactttcaat cattgtacac
     5281 tccgcctaaa aaaaataatc ttaaattagg cgttatatac aatctcccca ttacatattg
     5341 tataattaac ctttccatat aaagatttac ctttaaatgg tgaattatca gctttagaaa
     5401 caaagctttc aactgtccat tcttcttctg gattgaatat aacaatgtct gctacagccc
     5461 cttcttctat aaatccaata tttaaattgt acaactttgc aggatttata gacatttttt
     5521 ctattaactg catcatacta agatgatttt tatgcaccaa atttgttatt ccaagagcta
     5581 aagaagtttc aagcccaata attccacttg gagcttttat aaactctcta tctttttctt
     5641 ctttactatg aggtgcatgg tccgttgcta ttatttcaat agtaccatca tttaatccct
     5701 ctataatctt atctctgtcc catttacttc ttagaggtgg attcattttt gcatttgttc
     5761 caaactccaa tacatcttcc tcagtcaatg taaagtgatg tggtgaagct tctgcaacta
     5821 catttgcacc taagtatttt gcaaatctaa ccatatcaac agatacacct gaacttatat
     5881 gttgtatatc aactttagca ccagtcttta gtgccaacat acaatctctg gctaccataa
     5941 catcttctgc tacacttgat gcacctttta atccaagttc tgaagctacc tttcctgcat
     6001 ttacacctgg atttccaact aaagaagggt cttcttcatg aaaactaagt ggtacatcca
     6061 actcttttgc cttaatcatt gcctccataa ttagattgct atccattaaa ggtaatccat
     6121 catcagtaaa ccctggtaca ccagcttttt taagagcttc catatcaact aactctttac
     6181 cttcaaaacc tttagttata gctgctgctt gtaaaacatt tatacatgca ttttttgact
     6241 tttctttaac ataattaaaa gtatcttcat tatctacaat aggattagta tttgccatac
     6301 atataacagt tgtaaatcca cctctagcag cagatttagc tccactttct atatcttctt
     6361 tatatgtaaa cccagggtct ctaaagtgta catgtacatc cactaaacct ggagcaacta
     6421 cattaccact agcatctatt attttttcat attcatcact ttcatcaaac ttgcctattt
     6481 tataaatttt attttctttt ataattaagt caatattctc atctctttga cttttagggt
     6541 ctattactct accattttta attaaaatca tattatttct cctttatatt tttaatttat
     6601 atagactcat ataatattat acattatttt acatacttaa atacaaaatt acgtttgaca
     6661 tcaatagcct ttttaggaca aagttcatgg caacagtaac atcttataca tttacttaag
     6721 tctataatag cacccttagt ccctatctct atagtggatg caggacaagc ttccttgcat
     6781 tttccacatt ttatgcaatc ttgaaatctt accacaggct ttggtgttat aagctttgaa
     6841 ataggttcat ttaaaaattt tggtagcttt acagaaaaca atcttaaatc cttacttaca
     6901 tttggaatct tgtaattttt tataattaaa tcttttatat cttcacctaa aatttcaata
     6961 tctgaaaaat cttcttgtat atatcctctc ttaatagcac ctctaattgt agcaactcta
     7021 tttggaagta aacttataat cttacatgca acaacatcta tagcatatgg attttgtgat
     7081 gctaataaaa cgcctatttc tcgagtagta ccagctgaag gtccatcacc ttccatacct
     7141 actataccat ccataattgt caatgttggt tttatataat cacaaatatc taaaagtaca
     7201 ttttcgcaaa acaactcttc tgtaggaaat ctataatgta tctctgcttt tttcaatcca
     7261 gctatgcatc catataagtt ttttgtacca ccagtaaaag ttgccattat atgagtcttt
     7321 aatttacaaa gatttattat ataatctgaa tctactattg gtttaataat atccatatat
     7381 tttagaacat gtgctttttc actatgtatc ttaacatctg aaacatcata atttaactta
     7441 atgcccagtt catttgctac ttcttcaatt ccagatgcct tataaatgct cttaagagaa
     7501 ctttgattat atggaccacc agggctatct gctattgtaa tatcacaatt taactctaaa
     7561 agtttttcac atacaacttt tacaaccata ggatgtgtag ttgtagcttc ttcaggcttt
     7621 tttttcatta ataaatttgg ttttataagt actttgcttc cttttttaac aaacttatct
     7681 ataccaccaa tatcctctaa atttttttca atagataact ttacttcttc ataattgtaa
     7741 gttttacaac tcctaacaga taccgacttc atattatctc catccttcta tcacaacagt
     7801 ttatttgtat aatttaaaaa aggagaaacc aaagtttctc cttttaatct atgttttaat
     7861 tattattttc caaaatgtct gtttaataat cctaagaatg ctttaccatg tctagcttcg
     7921 tctttacaca tttcatgtac tgtatcatgt atagcatcta atcctaattc tttagctctc
     7981 ttagctaatt ttaattttcc atcagttgca ccatactcag catcaactct aactcttaag
     8041 ttttcttttg tatctgcaac tacaacttct ccaagaagtt ctgcaaattt agcagcatgt
     8101 tcagcctctt caaaagctat tctcttatac gcttcagcta cttctggata accttctcta
     8161 tcagcttgtc tactcattgc taaatacatt cctacttctg tacactcacc agtaaagttg
     8221 gctcttaatc cttcgattat ttctgcatct acaccttgag ctactcctat tctatgttca
     8281 tcagcccaaa ccatttcgcc tttcatttct tcaaatttat cagctccaac tttacatact
     8341 ggacattgtg ctggtgcagc atctccttca tgtatatatc cacatactgt acaaacaaat
     8401 tttttcataa ttaatttcct cccaatttta tataattaaa tttttatttt taagtttaat
     8461 aacttgctac aagaatatat ataccccaga ctttatagct taaacactaa aaataaaatt
     8521 tatttcctaa ttaattttat cactttgaaa caccagtatc aaccaagttt tgatgtcaaa
     8581 tttctacata atacataatt tatttgatta attaggaaat agttgtcata ttaaatactt
     8641 tctaatgaag actcctctga atctttaagt ttcacttttt tgctctttaa aaaagataat
     8701 ttagtttctg cagttatgat tgcaacaaat atcaagatac taccaataac caattttgct
     8761 gtaataagct cctttaaaat tattatagaa aatattgtac caaatacagc ctctgtggat
     8821 aatattatag ctgttctagt tccatcaact cttttttgac atatagtctg aaataaaaat
     8881 ccaattgtag tactaaatac agctagatac atagttccca tgtaaccaga taattcagct
     8941 tccatcttta attgacctgc aaatgtttga actacaacag acattaaaaa cgctactgta
     9001 aattgaatag ctgtcaatgc cattgggttg ttatcttttg caaactcact tgtaaagaat
     9061 atatgaaatg caaatccgaa tgaacatata agtgttagaa aatctccaaa attaatagag
     9121 aaatcagctt ctaaagaaag tatcccaatt cctataagag ctaccaaact acttattatt
     9181 cctattttat caagttttct tctataaagt ataaatccta tgaatggaac tattacaaca
     9241 ttagctgctg ttataaacgc atttttagat ggtgtagtgt acataagtcc aatagtttgt
     9301 actgtgaatg ctagaaataa aaatattcct aaaatccctc ctgcttttaa taatttttta
     9361 cccatattag cacgaatctg tttaaaaaac agtaaattca taattatagc tgccaagaaa
     9421 aatcttaaag ttatgatttg tagaggtgta agcccaccgt ctaatgcaaa ttgggttgct
     9481 acaaagccac ttccccatat aattgctata aatattagtc ctatttctcc aaagtatttt
     9541 ttcaataaag ccccctcctt tttatttttt tgcaaaatta taaaccccct aaacagcaag
     9601 agagtttttc tataagcatt actataaatt aatatagtaa attttttcag tattttaaat
     9661 ctaaatttac atacattttg agctcaaatt tatatccact atttaacagt aatatattaa
     9721 tcattttttg taaacacctt tttaata
//
LOCUS       DAMJYR010000131         9620 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.136, whole genome shotgun sequence.
ACCESSION   DAMJYR010000131 DAMJYR010000000
VERSION     DAMJYR010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9620)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9620)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9620
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.136"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            143..1507
                     /locus_tag="PC471_003124"
     CDS             143..1507
                     /locus_tag="PC471_003124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HDF2790232.1"
                     /translation="MGEKKINNMNYSHNNYSKKTGEPTRKIKNSHITLKSKEEKICSS
                     CNKENNTKDNYCKFCGNELYEIASLRPLETKLDLKSKIKELSYHANKRGVFLTTFTTI
                     FILFIIALIFKAIITIQFNDISYLINPAHIILALNLGQISVSMSTMMGSGFINANIGL
                     LILLIIPILVLLISNLIFMRKRCRDSKTVLANSLGVGIFYGLVLAILSIFTNVKTSSH
                     SMLEYGYALEYSYEFFSVLLNGFVLGFICTYITTYKKSYEKENMYLSLFSNSIRTFIL
                     GYVLVLVILLVLTISDSSYLNELDMSSYSNGLNLFTILPQIASYMWAFANGISVTIIN
                     STVSMFTLSSSSLFGDTKLMFYAMGALSMLILLLNGYKLRFKYNTDSIRPIIVFSIYY
                     AFLMGILALFSTFILDSNINFFNTTNYGTTLIMQFKVLQAIVISFVYSFVISLIGYKL
                     NSAD"
     gene            1529..2020
                     /locus_tag="PC471_003125"
     CDS             1529..2020
                     /locus_tag="PC471_003125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HDF2790233.1"
                     /translation="MEGNTNNKVDDKFSKVKKKFGSGKFKVELYKEKSKLNREIDDIQ
                     EKKAKIFLEMGILTYQKIREDSINDNSFDKFCDELLELDKLIYEKNMEINEMEMAESN
                     VTCECGYVGNVNDKYCAECGKKFELENECDDFIICGYCESEIDTEAEFCPCCGRKIIM
                     DLE"
     gene            2293..2964
                     /locus_tag="PC471_003126"
     CDS             2293..2964
                     /locus_tag="PC471_003126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HDF2790234.1"
                     /translation="MLDKVKGRLIVSCQALEDEPLHSPFIMGRMAKAAMEGGAVGIRA
                     QGVEDIIEIKKVTGLPVIGIIKRNYEDSDIYITPTKKEVDELLTTGCEMIALDATNRV
                     RPNNEDLKELIKYIKENGVLVMADISNYDEAIKAQEYGVDCVSTTLSGYTPYTKTLEG
                     PDFVLMERLVKDLEIPVIAEGKVNTPQDLKKVFELGVHSSVVGSAITRPQLITEKFVK
                     AIEIN"
     gene            3049..3915
                     /locus_tag="PC471_003127"
     CDS             3049..3915
                     /locus_tag="PC471_003127"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003452648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HDF2790235.1"
                     /translation="MKGIYSALLVSFDKEGNINEKGLRQIIRHNIDVCKVDGLYVGGS
                     TGENFMLSTDEKKRIFEIAKDEVKEEIKLIAQVGSVNLKEAVELAKFTTDLGYDAISA
                     VTPFYYKFDFEEIKHYYNTIINSVDNRLIIYSIPFLTGVDMSLDQFGELFENEKIIGV
                     KFTAADFYLLERMRKTFPNKLIFAGFDEMMLPATVLGVDGAIGSTFNVNGVRARQIFE
                     LTKNEKISEALEVQHVTNDLITDILGNGLYQTIKLLLEEQGVEAGYCRQPMKEATDEM
                     KSRAKEIYRKYF"
     gene            3948..5450
                     /locus_tag="PC471_003128"
     CDS             3948..5450
                     /locus_tag="PC471_003128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003462875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HDF2790236.1"
                     /translation="MVGFTWIDFAILVVYLLAVLFAGLLFSKKEMKGKEFFKGDGTIP
                     WWVTSVSIFATLLSPISFLSLAGNSYGGTWILWFAQLGMFIAIPLTIKFFLPLYSRLE
                     IDTAYEYLEMRYESKGLRVLGALMFIIYQIGRMSIIMYLPSIVLAGLTGIPVNVLIIV
                     MGVIAIIYSYTGGLKAVLWTDFIQGMVLIVGVTGTLFYLIASINGGFGTVMDTLTTGH
                     KFLASNEVMFDKNILSTSAFIIFVGAGLNTFSSYVSSQDIVQRFTTTTDIKQLNKMTY
                     GNGVLSMGLATVFYLIGTCLFIYYTQNPDLAQTVQQDQVFASYIAYELPVGITGILLA
                     AIYAASQSTLSTGLNSVATSWTLDIQTIITKDISMEKQTKIAQYISLGVGILSIAVAI
                     VLANGEIKSAYEWFNSFMGLVLGVLAGIFVLGAFCKKATKMGAYVGFIVSAILVVYLK
                     YRMPEVTSWAYSLITITTSVVVGTIVSAIEAKVKAKVSLPKAETTVYYEK"
     gene            5580..6032
                     /locus_tag="PC471_003129"
     CDS             5580..6032
                     /locus_tag="PC471_003129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HDF2790237.1"
                     /translation="MIFGNINHSKTYSTLHEDLLRCFEYAKDNSLIDYEKGTYTIDGD
                     DIFVNIVEYKTCEKEDRFWEAHKKYIDVHLMLDGCERIDINFIENLEQKIYEEKGDFL
                     PLDGKNNGYMELRKGDFLVCYPEDAHMTGIKVLEKENIKKAIFKVLVR"
     gene            6192..6995
                     /locus_tag="PC471_003130"
     CDS             6192..6995
                     /locus_tag="PC471_003130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2790238.1"
                     /translation="MENIDVFLLIGQSNARGLGNPKESIIPNENCFEYLSTDEIINMR
                     CELETSEGDGTIAPAFSNEWNKLTGNKVCFIHNAKDGSRIKNWNHDNNWFLNDTIEKF
                     NTGCTTLSKHYNIENKYVIWIQGESDAKYGSDVLYYKESLKKIAYRLKEECMIDKMFV
                     SLTGYWLGEDEYFIRTRRIAAAQESACSECDILCVGSKIAMQFHDRGLTIDDVHYTQE
                     GLNMLGEDLCKNIYKYHITKEKSVLSDTIDLSEARKYICELEKISKRFV"
     gene            7053..7940
                     /locus_tag="PC471_003131"
     CDS             7053..7940
                     /locus_tag="PC471_003131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HDF2790239.1"
                     /translation="MKNYVCIDVGGTSIKYGFIREDGFIIDKSSLDTEAKEKGGEGIL
                     AKIKDIVKKYIEENEISGICISTAGMVDPVEGKILFALEELIPNYKGMQLKKEVEKEF
                     NIKCEVENDVNCAGLGEMWLGAGKDATSSICLTIGTGIGGCIIINNKLINGFSNSAGE
                     VGYMNVNGSSFQELASTSSLIKKVAKIKNLNENDLNGKIIFDLAKNNDEDCLKELDNM
                     IKSLAVGIANICYVINPEVVILGGGIMAQEKFLKPKIDKALKEVLIERVYKNTNIEFA
                     KRQNDAGMLGALYNFLNKI"
     gene            7959..8807
                     /locus_tag="PC471_003132"
     CDS             7959..8807
                     /locus_tag="PC471_003132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDF2790240.1"
                     /translation="MSILEQLESPTFKVTKSDKLLIAYIKENIDDVFYKPISQIAKES
                     NIGEATITRFVKKMNFNGLQDFKVTLAQEISTINKKNIINKNIQNDEPALDTAKKLLS
                     SNVTTLENTVEIINSKDVHDCARLIINAKKVYFIGIGYSGIIAQDSNYKFMRIGLNCV
                     SFDSSHTMIMMSSIMEEGDLIIAISHSGETEEIIKTVKLARANNAKIISITENKNSEL
                     KDISDVHLSYVSGETVLETGSISSKLAQFFIIDLVYTQVVKELSNEAIERKIKTTNAI
                     KLFKNE"
     gene            8870..9559
                     /locus_tag="PC471_003133"
     CDS             8870..9559
                     /locus_tag="PC471_003133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HDF2790241.1"
                     /translation="MSKLKDYNIDKVIIFNNLSTKTKNQLKEKVKLKRLQKKEILFYE
                     KDSLDSVYILLEGKVSIFKISENGERKVIFILNSGEVINDITLDASKNSTVGCEAFEN
                     SIVAYYSIEEFLEIMQNDFQLTKDIISYMERRIRRLYRQLKNSISIKVDKKLAAKLYR
                     LSREFGVCCGEWTLLNVNITVTYLADMLGCKRETVSRMIKVLQKEELIKIDNKGFYVK
                     ETELSRYFKNN"
BASE COUNT         3882 a          910 c         1687 g         3141 t
ORIGIN      
        1 actatttaaa tatcaagacc tgttaaaaaa caacagcaat aaacaaataa aatagataaa
       61 tatatccaat taaggtataa ttaagtttta tatgatatag ttatatttga taatttacaa
      121 tagtaaaact tggagggaaa aaatgggtga aaagaaaata aataatatga attattcaca
      181 taataattat tccaaaaaga caggtgaacc aactagaaaa ataaaaaata gccatatcac
      241 tcttaaaagt aaagaagaaa aaatttgttc tagttgcaat aaagaaaata atactaagga
      301 taattactgt aaattttgtg gaaatgaact ttatgaaata gcatcattaa gaccacttga
      361 aacaaaacta gacttgaaat ctaaaataaa agagctttca tatcatgcaa ataaaagagg
      421 tgtattttta acaacattta caaccatatt tatattgttt attattgcat tgatatttaa
      481 agctattata actattcaat ttaatgatat tagttattta ataaatccag cacatataat
      541 cctagctctc aacttgggtc aaattagtgt atctatgtct accatgatgg gttctgggtt
      601 tataaatgcc aatataggtt tgttgatatt acttataatc cctattttgg tattattaat
      661 atcaaaccta atttttatga gaaaaagatg tagagattca aaaactgtat tagcaaattc
      721 tctaggagtt ggaatttttt atggattagt tttagctatt ttatctattt ttactaatgt
      781 aaaaactagt tcacatagta tgttagaata tggatatgca ttagaatata gttatgaatt
      841 ttttagtgtt cttttaaatg gatttgtatt agggtttata tgtacataca taactactta
      901 taaaaaaagt tatgaaaagg aaaatatgta tttatctctt ttttcaaatt ctataagaac
      961 attcatatta ggttatgttt tagtattagt aatactattg gtactaacta tatctgatag
     1021 tagttactta aatgaactag atatgagtag ttattcaaat ggattaaatt tatttactat
     1081 actcccacaa atagctagtt atatgtgggc ttttgcaaat ggaatttcag taaccataat
     1141 aaactcaaca gtatcaatgt ttactttaag ttcttcatct ttatttggag atactaaatt
     1201 aatgttttat gcaatgggag cattgtctat gttaatatta ttattaaatg gatacaagct
     1261 aagatttaag tataatacag atagtataag acctataata gtatttagta tatattatgc
     1321 atttttgatg ggaatattgg ctttatttag tacatttata ctagatagta atattaattt
     1381 ctttaataca actaattatg gaacaacttt aataatgcaa tttaaagtct tacaagctat
     1441 tgtaatttca tttgtatata gttttgtgat atctttaata ggatataagc taaattcagc
     1501 tgattaaata tgattggagg gaatttctgt ggaaggaaat actaataata aagtagatga
     1561 taaattttct aaggttaaga aaaagtttgg tagtggaaaa tttaaagttg aattatataa
     1621 agaaaaatca aaattaaata gagagattga tgatattcaa gaaaagaaag caaagatatt
     1681 cttggaaatg ggaatactga catatcaaaa aattagagaa gattctatta atgataatag
     1741 ttttgataaa ttctgtgatg agttactaga acttgataag ttaatttatg aaaaaaatat
     1801 ggaaattaat gaaatggaaa tggcagaaag taatgtaact tgtgagtgtg gttatgttgg
     1861 aaatgtaaat gataaatatt gtgctgaatg tggtaaaaaa tttgaattgg aaaatgaatg
     1921 tgatgatttt ataatttgtg gttattgtga aagtgagata gatacagagg ctgaattttg
     1981 tccatgctgt ggaagaaaaa ttataatgga tttagaataa tattgggaaa cctatgtata
     2041 gtatacatgg gtttttttat tgaaattatt taaatggact tataacagta gttatataaa
     2101 atgaaaataa acaccaaaaa aatgaaataa aatttctttt aaatgtaaaa aacaagctaa
     2161 tatataaaat aaatcagtaa aaatatagat gcgagaaaaa aatatgaaaa aatattgcaa
     2221 agaaaaagtt ttcatgttat tattaataca taaatcaaga aaataaattt cagaaacgag
     2281 gtaatttaaa aaatgctaga taaggtaaag ggaagattaa tagtatcatg ccaagcttta
     2341 gaagatgaac ctcttcatag tccttttata atggggagaa tggctaaggc tgctatggaa
     2401 ggtggagctg ttggaattag agctcaagga gttgaagata ttattgaaat aaaaaaagtt
     2461 actggacttc ctgttatagg tattataaag agaaactatg aagattcaga tatatatata
     2521 acgccaacta aaaaagaagt tgatgaactt ttaacaacag ggtgtgaaat gatagcatta
     2581 gatgctacca atagagttag accaaataat gaagacttaa aagaacttat aaagtatata
     2641 aaagaaaacg gtgttctagt aatggcagat atttcaaatt atgatgaggc tatcaaagca
     2701 caagagtacg gtgtagattg cgtttcaact acactatctg gatatactcc atatactaaa
     2761 acattagaag gtccagattt tgtattaatg gaaagattag ttaaagattt agaaatacct
     2821 gtaatagctg aaggaaaggt aaatactcct caagatttaa aaaaggtctt tgagttgggt
     2881 gttcattcat cagtagtagg ttcagcaata acaagaccac aattaataac agaaaaattt
     2941 gttaaagcaa ttgaaattaa ttaaaaatta atttataaat taaaaaatta ttatatttaa
     3001 ctatatttga aaattaaaat tggaggtaaa gatatgagaa caacagatat gaagggaatt
     3061 tattcagcat tattagtttc atttgataag gaaggaaata taaatgaaaa agggttaaga
     3121 caaataatta gacacaatat agatgtttgt aaagtagatg gtctttatgt aggaggaagt
     3181 acaggggaaa acttcatgct ttcaactgat gaaaagaaaa gaatatttga aatagctaaa
     3241 gatgaagtga aagaagaaat aaaattaata gctcaagttg gttcagttaa tttaaaagaa
     3301 gctgtagaac ttgctaaatt tacaactgat ttaggatatg atgcaataag tgctgttaca
     3361 cctttttact ataaatttga ttttgaagaa ataaaacact attataatac aataataaat
     3421 tctgtagata atagattaat aatatactct ataccatttt taactggtgt tgatatgagt
     3481 ttagaccagt ttggagaatt atttgaaaat gaaaaaataa taggtgttaa gtttacagca
     3541 gcagatttct acttattaga gagaatgaga aaaacattcc caaataaatt aatatttgca
     3601 ggatttgatg aaatgatgtt accagctaca gttttaggtg tagatggggc aattggttct
     3661 acattcaatg taaacggtgt cagagctaga caaatattcg aattaactaa gaatgaaaaa
     3721 atatcagaag cacttgaagt acaacatgtg actaatgatt taataacaga tatattagga
     3781 aatggcttat atcaaacaat taaattacta cttgaagaac aaggtgttga ggctggatac
     3841 tgtagacaac ctatgaaaga agcaactgat gaaatgaaat caagagcaaa agaaatatac
     3901 agaaagtatt tttaataaaa tacttttagt atgggaggat tagtcaaatg gtaggattta
     3961 cttggataga ttttgcaata ctagtggttt acttattggc agttttattt gcaggattgt
     4021 tattttcaaa aaaggaaatg aagggaaaag agttcttcaa aggtgatgga actattccgt
     4081 ggtgggttac atcagtatca atatttgcca cattattaag tccaatatca tttttatcat
     4141 tagcaggaaa ttcttatggt ggtacttgga ttttgtggtt tgcacaatta ggtatgttta
     4201 tagcaatacc tttgacaata aaatttttct tacctctata tagtaggtta gaaatagata
     4261 cagcatatga atacttagaa atgagatacg aaagcaaagg tcttagagta cttggagctt
     4321 tgatgtttat aatatatcaa ataggacgta tgtctatcat aatgtatctt ccatcaatag
     4381 ttctggctgg tcttacagga atacctgtaa atgtactgat aatagttatg ggtgtaatag
     4441 caataattta ctcatataca ggtggattaa aggctgtctt gtggaccgat tttatacaag
     4501 gaatggtatt aatagtaggt gttacaggga cattgttcta tttaatagca agtataaatg
     4561 gtggttttgg aacagtaatg gatactctta caactggaca caagttctta gcaagtaatg
     4621 aagttatgtt tgataaaaat atactatcta caagtgcatt tataatattt gttggagctg
     4681 gtcttaatac tttttcatca tatgtatcga gtcaagacat agttcaaaga tttactacaa
     4741 caactgatat aaagcaactt aataaaatga catatggaaa tggtgtttta tcaatgggac
     4801 ttgcaacagt attttatcta attggaactt gtttatttat atactatact caaaacccag
     4861 atttagctca aacagttcaa caagaccaag tatttgcatc atatatagct tatgaattac
     4921 cagtgggtat aacaggaata ctacttgcag cgatatatgc agcttctcaa tcaactttat
     4981 cgacagggct taattcagtt gctacaagtt ggactttaga tatacaaact atcataacaa
     5041 aagatataag tatggaaaag caaactaaga tagcacaata tatatcatta ggtgtaggta
     5101 tactttctat agctgttgca atagtattag caaatggaga aataaagtca gcatatgaat
     5161 ggtttaatag ttttatgggg ctagtactag gtgttttagc aggaatattt gtacttggtg
     5221 cattttgtaa aaaagcgact aaaatgggag catatgttgg attcatagta tcagcaattt
     5281 tagttgttta tttaaaatac cgtatgccag aagtaacttc ttgggcatat tctctaataa
     5341 caatcacgac ttctgtagtg gtaggtacaa tagttagtgc aatagaagct aaagttaaag
     5401 caaaagtatc tttaccaaaa gctgagacta ctgtttatta tgaaaaataa tatttaaatg
     5461 ataaattgtc ttatagagtg attagataaa taatttagtt actctataag attgtataat
     5521 atactttgta tataaaagta taaaaataga ctttataatt atatagggag atagtgctta
     5581 tgatatttgg aaatataaat catagtaaga catattcaac attgcatgaa gatttactta
     5641 gatgttttga atatgctaaa gataattcat taatagatta tgaaaaaggt acttatacaa
     5701 tagatggtga tgatattttt gtgaatattg tagagtataa aacttgtgaa aaagaagata
     5761 gattctggga agctcataag aagtatatag atgttcattt gatgcttgat ggatgtgaac
     5821 gaatagatat aaattttata gaaaatttag aacaaaaaat atatgaagaa aagggcgatt
     5881 tcttgcctct tgatggtaaa aataatggat acatggaact tagaaaaggt gattttttag
     5941 tttgttatcc ggaagatgct catatgacag gaataaaagt tttagaaaaa gaaaatatca
     6001 aaaaagctat ttttaaggtt ttggttagat aatgataatt tatataacca ctagggctga
     6061 aaatagtgat ttaactttca gcgattagtg agacttcgta aattttagtt agattactat
     6121 tttcagcctt agttttttat gagaaaattg aaaataatta aaagcttaaa attttgtaaa
     6181 ggagttaact tatggaaaat atagatgtat tcttattaat aggacaaagc aatgctagag
     6241 gattgggaaa tccaaaagag agcatcattc caaatgaaaa ttgttttgaa tatttgagta
     6301 ctgatgaaat tataaatatg agatgtgagc tagagacttc agaaggtgat ggtactatag
     6361 cacctgcatt ttcaaatgaa tggaataagc ttactggaaa taaagtttgt tttattcaca
     6421 atgcaaaaga tggttcaaga ataaaaaact ggaatcatga taataactgg tttctaaatg
     6481 acactataga aaaatttaat acaggctgta ctaccttaag taaacattat aatatagaaa
     6541 ataaatacgt tatttggatt caaggtgaaa gtgatgcaaa atatggtagt gatgtattgt
     6601 actataaaga aagtctaaaa aaaattgcgt ataggcttaa agaagaatgt atgattgata
     6661 aaatgtttgt aagtttaact ggttattggc ttggagaaga tgaatatttt attagaacaa
     6721 gacgaattgc agcagcacag gagtcggctt gtagtgaatg tgatatatta tgtgtaggaa
     6781 gcaaaattgc tatgcagttt catgacagag gattaactat tgatgatgtt cattacactc
     6841 aagaaggtct taatatgtta ggagaggact tatgtaaaaa tatttataag tatcatataa
     6901 ccaaagaaaa aagtgtatta agtgatacta tagacttatc agaagcaagg aaatatatct
     6961 gtgaattgga aaagataagt aaaagatttg tgtaatataa aattttaaat agagttgtta
     7021 atttataaaa ataatttgta tggagatatg ttatgaaaaa ttacgtttgt atagatgttg
     7081 ggggtacttc aataaaatat ggttttatta gagaagatgg atttataatt gataaatcaa
     7141 gtcttgatac tgaagcaaag gaaaaaggtg gagaaggaat attagccaaa ataaaagata
     7201 tagtaaaaaa atatatagag gaaaatgaaa taagtggtat atgtatatca actgcaggta
     7261 tggttgaccc agttgaaggt aaaatactat ttgcattaga agaattaatt ccaaactata
     7321 agggcatgca attaaaaaaa gaagtagaaa aagagtttaa tataaaatgt gaagttgaaa
     7381 atgatgttaa ttgtgctgga cttggtgaaa tgtggcttgg agcaggtaaa gatgcaacat
     7441 cttctatatg tttaacaata ggtactggaa ttggtggatg tattataata aataataaac
     7501 tgataaatgg gtttagtaat agtgctggtg aagtaggata tatgaatgta aatggaagtt
     7561 cttttcaaga attggcatct actagcagtc taattaaaaa agtagcaaaa ataaaaaatt
     7621 tgaatgaaaa tgacttaaat ggaaaaataa tttttgattt agcaaagaat aatgatgaag
     7681 attgtttaaa agaactcgat aatatgataa aatcattagc agttggaatc gcaaatatat
     7741 gttatgtaat aaatccagaa gtggtaattt taggtggtgg gattatggca caagaaaagt
     7801 tcctaaaacc aaaaatagac aaagcattaa aggaggttct tatagagaga gtttacaaaa
     7861 acactaatat agaatttgca aagagacaaa atgatgcagg aatgcttgga gcattatata
     7921 attttttaaa caaaatataa agaatagaag ggtttttaat gagtatttta gaacaattgg
     7981 aatcaccaac ttttaaagtc acaaaatcag ataaattatt aatagcttat ataaaagaaa
     8041 atatagatga tgtattttat aaaccaatat cacaaatagc taaagagagc aatattggtg
     8101 aagcaactat aactaggttt gtaaaaaaaa tgaattttaa tggattacag gattttaaag
     8161 ttactttagc acaagaaata tcaactatca ataaaaaaaa tattataaat aaaaatatac
     8221 aaaatgatga gccagctttg gatacagcaa aaaaactttt aagttcaaat gtaaccactt
     8281 tagagaacac agtagagata atcaatagta aggatgtaca tgactgcgca aggcttataa
     8341 taaatgcaaa gaaagtttac ttcataggta ttggatattc tggtataata gcacaggatt
     8401 caaactataa atttatgaga ataggtctta attgtgtaag ttttgacagt agtcatacaa
     8461 tgataatgat gtcttccata atggaagaag gagatttaat aattgcaatt tcacattctg
     8521 gtgaaacaga agagattata aaaactgtaa agcttgcaag agccaataat gctaaaatta
     8581 tatcaattac tgaaaataaa aattctgaat taaaggatat ttcagatgta cacttatctt
     8641 atgtatcggg agaaacagta ctggaaactg gttcaatatc ttctaaatta gcacaatttt
     8701 tcattattga tttagtatat acacaggtag taaaagaact ttctaatgaa gctattgaaa
     8761 ggaaaattaa aactacaaat gctataaaat tgtttaaaaa tgaataaaat aagaaatttg
     8821 taatgtttta tagagctctc ttaaaaattt aggaatggag agatattaca tgtctaaatt
     8881 aaaagattat aatattgata aagtaataat ttttaataat ttaagtacta aaacgaagaa
     8941 tcaacttaaa gaaaaagtta aactaaaaag attacaaaaa aaagaaattt tattttatga
     9001 aaaagatagc ttagacagtg tttatatact tttagaagga aaggtatcta tttttaagat
     9061 aagtgaaaat ggagagagaa aagtaatttt catattaaat agtggggaag tcataaatga
     9121 tattacactt gacgcttcta aaaactctac tgttggctgt gaagcatttg aaaattcaat
     9181 tgtagcttat tatagtattg aagaattttt agaaattatg caaaatgatt ttcaacttac
     9241 aaaggatatt atttcttata tggaaagaag aataagacgt ttatatagac agttgaaaaa
     9301 ttctatatcc ataaaagtag ataaaaaact tgcagcaaaa ttatataggt taagtagaga
     9361 atttggagtt tgctgtggag aatggacttt actaaatgta aatattacag ttacatattt
     9421 agctgatatg cttggctgta aaagggagac tgtttccaga atgataaagg tattgcaaaa
     9481 agaagaactt attaaaattg acaataaagg tttttatgtt aaagaaactg aattatcaag
     9541 gtattttaaa aataattaat tatgattaag taagagtatt attagaattt taattaaata
     9601 aaaatattat taagagaatc
//
LOCUS       DAMJYR010000132         9532 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.137, whole genome shotgun sequence.
ACCESSION   DAMJYR010000132 DAMJYR010000000
VERSION     DAMJYR010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9532
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.137"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            58..729
                     /locus_tag="PC471_003134"
     CDS             58..729
                     /locus_tag="PC471_003134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2790242.1"
                     /translation="MNLLIIEDDINLNEGLFYAFENDGFNVFKAYTKQEGLNIFNSKN
                     IDFIILDCNLPDGDGFDVCQIIREKSDIPIIMLTARDSEIDEVKGLEIGLDDYITKPF
                     SLSVLKARVKVAIRKKSNNKVIYSNGIKLDQKLLKVYKNKECLELSSVEYKLLSYLIE
                     NKGQILLKEQILHHIWDSEENYVDDNIVSVNIRRLRVKVEDDPSNPKYIKTAYGMGYL
                     WNEVE"
     gene            731..1639
                     /locus_tag="PC471_003135"
     CDS             731..1639
                     /locus_tag="PC471_003135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF2790243.1"
                     /translation="MFILAFIICTVVIFIVSTKINRKRYDELNLMLNQILEGKEVTYP
                     DTKDTRASKISHQVKKIKDMIEIEVEQSKSEKEAIKGLISNMSHQLKTPLSNITIYCE
                     LLENINISTLQKKEFLQKMKNETFKIDWLLQSLFKMTKLEDGVIEFEVEELLIKDTLI
                     QSISTIFNKAEAKNIRINLEPFSDIKLVHNKKWTIEAIVNVLENAIKYSPSDSTITIS
                     VIKMELYTKITIKDEGIGIDSRELNDIFKRFYRSKNVENQNGTGIGLYLTRLILEKEN
                     GNIIVESKLGSGCCFSIFLQNCKSLN"
     gene            1716..2402
                     /locus_tag="PC471_003136"
     CDS             1716..2402
                     /locus_tag="PC471_003136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2790244.1"
                     /translation="MSILVTNNLVKHYGKGETKVKALNGVSIEIERDTFTAIVGTSGS
                     GKSTLLNIIGGLDNPTSGDVIIKGKNISKIGKKDLTVFRRRNIGFIFQNYSLMPVLNT
                     YDNIALPVTLDKGNHIDHEYIEMLMKTLGIWDKRLKFPSELSGGQQQRVAIARALANK
                     PALILADEPTGNLDSKTTMEVVCLLKESSAKFHQTILMVTHNENIAQVCDSIIHIEDG
                     VVVNTGGEVL"
     gene            2399..4954
                     /locus_tag="PC471_003137"
     CDS             2399..4954
                     /locus_tag="PC471_003137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HDF2790245.1"
                     /translation="MMSLVKFSIQYIKHYKKQSISIVLSIVLSVALLTGIGSLVHSAD
                     KSRIEKIREDSGDYHYFYKVDKEQLNKIKKNKKSKEYTVDRLGVTKPKNQIDEPFIIE
                     FLNADSSYLDMTGRKLLKGTLPSKDGEIVLDTYSLNNLNVNKEIGTEINLDGKIYKLC
                     GILSSLSNPETALEGFVNDYFQPKEKDSYIVYVKYDENKNIKKQNYKFMKKFNISKEK
                     TYINWPLSVEFGVKPPKNIEKYNIFEFLKNLELNTNAIILLVGVFGAAAVYSIFHVSI
                     LQRISQYGVLEVLGANNKQLLFLLFLELFLLFIVGFPIGCFLGIEVASTIYEQFPHIF
                     LSSDIVPGAFFISEKSIYLGFLFILLLLILIAVRVVHQLNKYSSIESMKNFKITNKKK
                     RILESVKHSNMTKVLSHKYMTQKKGMFIGILFSLSLGGIIFLCSSYSIQLTKNNNELT
                     MKADDGLNSDYQITMQTTDFDVGIPKDKISRLNNVEGISKVYPVRYLFGAMQIKEEQL
                     FWKNFFKPLEKDYRIKTFFDGICTKQNEGGYLLKTNIYGYNNGMLEGLNSYIIDGEIN
                     THDMVKNNKVIVRLPMDGTGMYDAVDINPGDTIKVKVPKTMKPTDEIVKFKEENKNDY
                     TTKEFVVAATVKRVMANNIYFIGDYGMDIVMTNGQMKNNFDIENYNSVSIKKTEDSNS
                     EELSENIKSVVSDVKRCIVTDYTIAIEKNNTYLNQKLLFIYGIVFVLLAISLFHIINT
                     VSYLIFSRRHEFGILRAMGITDNKFLLMMIREGFLYGFYASIIMVIGSVIGQFMIYFM
                     VKRVYLYINPILKINTPLYIGMIILNITISIVAVIIPVRQILKSDIISEINKN"
     gene            complement(5148..6347)
                     /locus_tag="PC471_003138"
     CDS             complement(5148..6347)
                     /locus_tag="PC471_003138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003493023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420-0:L-glutamate ligase"
                     /protein_id="HDF2790246.1"
                     /translation="MDRLVGTVSRGVRAPIIRQGDDLVKIVVDSVLNASKSENFEVRD
                     KDVIAVTEAVVARAQGNYAHVDNIAKDVKDKFGDDTVGVIFPILSRNRFAICLKGIAK
                     GCRKVVLMLSYPSDEVGNHLISIDELDDKGINPWSDVLTEEKYRELFGYNKHTFTGVD
                     YVDYYKNLIKDCGAEVEIVFANNPKTILNYTTSILTCDIHTRQRTKRILRQNGAKKVY
                     SLDDIMTASVDGSGYNDQYGLLGSNKATEETVKLFPINCDEVVNKIQGNIKEITGKDV
                     EVMVYGDGAFKDPVGKIWELADPVVSPAYTKGLEGTPNEVKLKYLADNDFADLSGDEL
                     KEAISKYIVEKDNKSDDLTGNMVSQGTTPRRLTDLIGSLADLTSGSGDKGTPIIYIQG
                     YFDNYTK"
     gene            6466..7353
                     /locus_tag="PC471_003139"
     CDS             6466..7353
                     /locus_tag="PC471_003139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDF2790247.1"
                     /translation="MNIKLELYKVFNAVVNNKSFSLAAKELFMSQPAVSQSIKQLEEQ
                     LDTLLFYRNNKGVKLTPEGKILSEHVTTALKLISSGEDRINKFKKLEYGSLKIGVGDT
                     AARFFLLKYLEIFHKKYPHIHVSTINRTSRELISLLKDGNIDIAIINMPIEDDTLNIV
                     ECIEIHDIFVCANDYIEYKGRKISLEELNTLPLIMLENKANSRLYVNEYFLSKGIKLN
                     PDIELGSHELLLEFAYINLGVSCVIEEFSIDYLENEKLFKLDIKEPIPKRNIGYCHLK
                     DISLSLATKEFLSMISNNI"
     gene            7543..8100
                     /locus_tag="PC471_003140"
     CDS             7543..8100
                     /locus_tag="PC471_003140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDF2790248.1"
                     /translation="MSDSEYPQMKTLETRSCILRPASLDDVEDFFDCYKNKIVVKHLP
                     FSEHKSLEDTRKFIKSFFLNPYKKGKIGHFAIVYKRDNKVIGNMGFNNINPKALEAEI
                     GICINPSYWGHDFATEITKRVIQYGFRELNLNKIIAITYEENQNSTKSLDLLGFKCTG
                     KYIKKIHTGNTVKNVPCYQYELKKQ"
     gene            complement(8339..9199)
                     /locus_tag="PC471_003141"
     CDS             complement(8339..9199)
                     /locus_tag="PC471_003141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HDF2790249.1"
                     /translation="MKKQKSPILLEMLGLFFGCISMSIGINMFLKPHTIAPGGLSGLS
                     LVLNKVTGLPVSAIMLIIGVPLVILAFRIMGTKNSLKTLFGTVVFSAIVQLTDPLSKL
                     RFTNDLLLSSIAGGILVGIGLGIMFKSDASTGGTDLIALILSKKFPGIKATKFMSCLD
                     GMVVISSGIVNRSLETALYSGIALCVLIKIADMIMEGFDHSKAFFIISDEPESLRQAI
                     TEELDRGLTILDGKGGYTRENKEVLLVVVSKKQELYLKRLVKRTDPTAFVIVTDVHEV
                     LGEGFKNLEN"
BASE COUNT         3705 a         1155 c         1403 g         3269 t
ORIGIN      
        1 tagtatgata atgcaagaaa ataattattt atctaaaaaa tctaaaggag agtaaaattg
       61 aacttattaa ttattgaaga tgatataaac ttaaatgaag gtctttttta tgcatttgag
      121 aatgatggat ttaatgtatt taaggcttat acaaaacaag aaggattaaa tatatttaat
      181 agtaaaaata tagattttat aatcttagac tgcaatcttc cagatggaga tggatttgat
      241 gtatgtcaaa taataaggga aaagtcagat ataccaatta taatgcttac tgcaagagac
      301 tcagaaattg atgaagtaaa aggattagaa atagggcttg atgattacat tacaaaacca
      361 ttttctttat ctgtattaaa ggcaagagtc aaagtagcaa taagaaaaaa atcgaataat
      421 aaagtaatat attccaatgg aataaaattg gaccaaaagt tactaaaagt atataaaaat
      481 aaagaatgtt tggaacttag ctctgtagaa tataagcttc taagttatct aattgaaaat
      541 aaaggtcaaa tattattaaa ggaacaaatt ttacaccata tttgggacag cgaagaaaat
      601 tatgttgatg ataatattgt atctgtaaat ataagaagac ttagagtaaa agtagaggat
      661 gacccatcta atccaaaata tataaaaaca gcttatggaa tgggttattt atggaatgag
      721 gtagaataat atgtttatat tagcctttat aatatgtaca gttgttatat ttatagtatc
      781 tactaaaata aatcgaaaaa gatatgatga attaaatttg atgttaaatc aaatattgga
      841 aggtaaagaa gttacttatc cagatacaaa ggatacaaga gcgtcaaaaa tttctcacca
      901 agtaaaaaaa attaaggata tgatagaaat agaggtagaa cagtcaaagt cagaaaaaga
      961 agcgattaaa ggtttaatat caaatatgtc acatcaatta aaaactccac tttcaaatat
     1021 aaccatttat tgtgaacttc tggagaatat aaatatttcc acattacaga agaaggagtt
     1081 tttacaaaag atgaaaaatg aaacttttaa gattgactgg ctgttacaat ccttatttaa
     1141 aatgactaaa ttagaggatg gagttataga atttgaagta gaagaactac taattaaaga
     1201 tactcttatt caaagtatca gtactatctt taataaagca gaagctaaaa atataaggat
     1261 aaatctcgaa ccattttcag acataaaatt agttcataat aaaaagtgga ccatagaagc
     1321 aattgtaaat gttttagaaa atgcaataaa atactcacca tcagatagca cgattactat
     1381 ttctgtaata aaaatggaac tatatacgaa gattactatt aaagatgagg gaataggtat
     1441 tgatagtaga gaactaaatg atatttttaa aagattttac agaagtaaaa atgttgaaaa
     1501 ccaaaatggc acagggattg gtctatatct tacaagatta attttagaaa aagaaaatgg
     1561 aaacattata gtagaatcta aactaggaag tggatgttgt tttagtatct tcttacagaa
     1621 ttgtaagagt ttaaactaat tttgtaggat gagtgtaatt atcgtcactt acaatatttt
     1681 atagaacata tatctgtatt gaaaggagaa attaaatgag tatattagtg acgaataatt
     1741 tagtaaaaca ctatggaaaa ggtgagacta aagtaaaagc cttaaatgga gtaagtatag
     1801 aaattgaacg agatacattt actgctatag ttggaacttc tggttctgga aaaagcacat
     1861 tgttaaatat tataggtgga ttagataatc caacctcagg tgatgtaata attaaaggaa
     1921 agaacatatc taaaatagga aaaaaagatt tgacagtgtt tagaagaaga aatataggat
     1981 ttatatttca aaactacagt ttaatgcccg tattaaacac ttatgataat atagctcttc
     2041 ctgttacatt agataaagga aaccatatag accatgaata tattgaaatg ctgatgaaga
     2101 cattgggaat ttgggataag cgtttaaaat ttccaagcga actatctggt ggtcaacaac
     2161 agagggtagc aattgctaga gctttggcaa ataaacctgc gcttatatta gcagatgaac
     2221 caacaggaaa tctcgatagt aaaactacaa tggaagtggt atgtttatta aaagaaagta
     2281 gtgcaaaatt ccatcaaacg atacttatgg taacacacaa tgaaaatata gcacaagtat
     2341 gcgactcaat tatccatatt gaagatggag ttgtggtgaa tactggtggt gaagtactat
     2401 gatgtctcta gtaaaatttt caattcaata tataaagcat tataaaaaac aatctatatc
     2461 tatagtttta agtatagtac tatcagtagc attattgacg ggaattggtt ctttagttca
     2521 tagtgcagac aaaagtagaa ttgaaaagat aagggaagat agtggagatt atcattattt
     2581 ctataaagta gataaggaac aacttaataa aattaaaaaa aataaaaaat caaaggaata
     2641 cactgtagat agacttggcg ttacaaaacc taaaaatcag atagatgaac cttttattat
     2701 agaattttta aatgctgatt cttcatattt agacatgaca ggtagaaaat tattaaaagg
     2761 aactcttcct agtaaagatg gagagatagt acttgacaca tattctctaa acaatttaaa
     2821 tgtaaacaaa gaaattggaa cagaaatcaa tttagatggt aaaatctata agttatgtgg
     2881 tattttatca agtctttcaa acccagaaac tgctctggag ggatttgtaa acgattattt
     2941 tcagcctaaa gaaaaggatt cttatatagt ttatgtaaaa tatgatgaaa ataaaaatat
     3001 taaaaagcaa aattataaat ttatgaaaaa attcaacata tcaaaagaaa aaacttatat
     3061 aaactggcct ctttcagttg aatttggagt aaaaccacca aaaaatatag aaaaatataa
     3121 tatatttgag ttcttaaaaa atttagaact aaatacaaat gcaattatat tacttgttgg
     3181 agtgtttggt gcagcagcag tatatagtat tttccatgtt tcaatacttc agagaatatc
     3241 acaatatggg gttttagaag tattaggcgc taataataaa caacttttat ttctactatt
     3301 tttagagtta tttttacttt ttattgttgg atttcctata ggttgcttct taggtattga
     3361 agtggcgtct acaatatatg aacaatttcc acatatattt ttgagtagtg atattgttcc
     3421 aggagcattt tttataagtg aaaaatctat ttatttaggc tttttattta tcttattgtt
     3481 gttaatttta attgctgtac gtgttgtaca tcagttgaat aaatactcta gtatagaatc
     3541 tatgaaaaac tttaagatta ctaataagaa aaagagaata ttagaaagtg taaaacactc
     3601 taacatgaca aaagtattat ctcataaata catgactcaa aaaaagggaa tgtttattgg
     3661 aattttgttt tcattatcac ttggtggaat tatattttta tgttctagtt actctataca
     3721 gcttactaaa aataataatg aacttacaat gaaagctgat gatggtttaa attctgacta
     3781 ccaaataact atgcaaacaa cagattttga tgtaggtata ccaaaagata aaatttcaag
     3841 attaaacaat gttgagggaa tatcaaaagt ctatccagta agatatttat ttggagctat
     3901 gcaaattaaa gaagaacaac tattttggaa aaatttcttt aaacctcttg aaaaagatta
     3961 caggataaag actttttttg atggtatttg tacaaagcaa aatgaaggtg gctatctttt
     4021 aaaaacaaat atttatggtt ataacaatgg tatgttagaa ggattaaatt cttatattat
     4081 agatggggag attaataccc atgatatggt taaaaataat aaggttatag ttagactacc
     4141 tatggatgga actgggatgt atgatgcagt tgatataaat ccaggagata caataaaagt
     4201 taaagtgcca aagacaatga aaccaacaga tgaaattgtt aaatttaaag aagaaaataa
     4261 gaatgattat acaacaaaag aatttgtagt tgctgctact gtaaaacgtg tgatggcaaa
     4321 taacatatat ttcattggtg attatggtat ggatatagtt atgacgaatg ggcaaatgaa
     4381 aaataacttt gatatcgaaa attataattc agtaagtata aaaaagacag aagatagtaa
     4441 cagtgaggaa ttgtctgaga acataaaaag tgttgtaagt gatgtaaaaa gatgtatcgt
     4501 aacagattat accattgcta ttgaaaaaaa taatacctac ttaaatcaaa aactcttatt
     4561 tatatatggt attgtatttg tacttcttgc aataagtttg ttccatataa tcaatacagt
     4621 aagctactta attttttcaa gaagacatga gtttggaatt ttaagagcta tgggtattac
     4681 ggataataag tttttactta tgatgataag agaaggtttt ttatatggat tctatgcaag
     4741 tattataatg gtaattggaa gtgttattgg acaatttatg atttatttta tggtaaaacg
     4801 tgtatattta tatataaatc caattcttaa aataaataca cctctataca taggtatgat
     4861 tattttaaat atcactatta gtatagttgc tgtaattatt ccagtaagac aaattttgaa
     4921 gagtgatatt atttcagaaa taaataagaa ttagagttat ataaattttt aagattatat
     4981 aactttctga caatcttgtt taaattattt aaatatatct tatctgtgaa cattaaataa
     5041 acctcttaat agatataact cttgaaaagt catatctatt aagaggtttt aataaatttt
     5101 catgttaata tatataatcc aaaaaaatta aatcttatta aaacatacta ttttgtatag
     5161 ttatcgaaat atccttgtat gtaaataata ggagtaccct tatctccact tccagatgtt
     5221 aaatctgcta aagaacctat aaggtctgtt aatcttcttg gagtagtacc ttgagaaacc
     5281 atgtttcctg ttaaatcatc agatttatta tctttttcaa ctatgtattt tgaaattgct
     5341 tcttttaact catcaccaga taagtcagca aaatcattat ctgctaagta ttttaatttt
     5401 acttcgtttg gagttccctc tagccctttt gtataagcag gagatactac tgggtcagct
     5461 aattcccata ttttacctac agggtcttta aaagctccat ctccataaac cattacttct
     5521 acatctttcc ctgttatttc ttttatatta ccttggattt tgtttacaac ttcatcacaa
     5581 tttataggga ataattttac tgtttcttct gtagctttgt ttgaacctaa aagtccatac
     5641 tggtcattgt aaccactacc atctacactt gctgtcatta tatcatcaag tgaatatact
     5701 tttttagctc cattttgtct taaaattctc tttgttcttt gtcttgtatg tatatcacaa
     5761 gttaaaatac ttgttgtata gttaagaata gtttttggat tgtttgcaaa tactatttct
     5821 acctctgcac cacaatcctt gattaaattc ttataataat caacataatc tacaccagta
     5881 aatgtatgct tgttatatcc aaatagttct ctgtattttt cttctgttaa aacatctgac
     5941 caaggattta ttcccttatc atcaagttca tcaatactta ttaagtgatt tccaacttca
     6001 tcagaagggt agcttaacat taatacgact tttctacatc cttttgcaat tccttttaaa
     6061 catatagcaa aacgatttct acttaaaatt gggaatataa ctccaacagt atcatctcca
     6121 aatttatctt ttacatcttt tgcaatattg tcaacgtgag cataatttcc ctgtgctcta
     6181 gctacaacag cttctgtaac ggcaattaca tctttgtccc taacttcaaa gttttcactt
     6241 tttgacgcat ttaaaacaga atcaacaact attttaacta agtcatctcc ttgtcttata
     6301 ataggtgctc taacccctct tgaaacagtt ccaactaatc tatccattat ctaaaatcct
     6361 cctaaagaat taatattgtc atattagtac catgtatatt ataattcaaa tataattata
     6421 agtaaaataa ataatactaa tatttgacat aaggagtaat tatatatgaa tataaaactt
     6481 gaactttata aggtttttaa tgcagttgta aataataaga gtttttctct tgctgctaag
     6541 gaattgttta tgtcacagcc agctgtaagc cagtcaataa agcaattaga agaacaatta
     6601 gatactttat tattttatag aaacaataaa ggagtaaaat taactccaga ggggaaaatt
     6661 ctaagtgaac atgtcactac tgcattaaag ttaatatcat caggagaaga tagaataaat
     6721 aaatttaaaa aattagaata cggttctctg aaaataggag taggagatac agctgcacgt
     6781 tttttcttgt taaaatattt agaaattttt cataagaagt atccacatat acatgtatct
     6841 acaataaata gaacgagtag agaacttata tctttactca aagatgggaa tatagatata
     6901 gcaattataa acatgccaat agaagatgat acactaaata ttgttgagtg tattgaaata
     6961 catgatatat ttgtatgtgc aaatgattac atagaataca aaggtaggaa aatatcattg
     7021 gaagaattaa acacattgcc tttaataatg ctagaaaata aagcaaattc aagactttat
     7081 gtaaatgaat attttttatc aaaaggtata aaactaaacc cagatataga attgggttct
     7141 catgaattac ttttagaatt tgcgtatata aatcttggag tgtcttgtgt tatagaagaa
     7201 tttagcatag attatttgga aaatgagaaa ttgtttaaat tagatataaa agagccaata
     7261 cctaagagaa atataggtta ttgccattta aaagatatat ctttatcctt agcaaccaaa
     7321 gaatttttaa gcatgatatc taataatata taatttagaa tacatatttc acaagtaatt
     7381 taatataaaa atagagataa attcgtaaat ttgaaagatt catatagtaa ttagttttta
     7441 ttaaaaataa aaataaggtt aatttgagaa catttatata attaatatcg tattttaaat
     7501 atgaacagta tatttagatt aatgtataaa gggggattag aaatgtctga ttctgaatat
     7561 cctcaaatga aaaccttaga aactagaagt tgtatactaa gacctgcatc attagatgat
     7621 gttgaagatt tttttgactg ttataaaaat aaaatagtag taaaacattt accatttagt
     7681 gagcataaat ctttagaaga tacaagaaag tttataaaat cattttttct aaatccatat
     7741 aaaaaaggga aaattggcca ctttgctata gtatataaaa gagacaataa agttattggg
     7801 aatatggggt ttaataatat aaatcctaaa gcattagaag cagaaatagg tatatgtata
     7861 aatccaagct attggggaca tgattttgct actgagataa caaaaagagt aatacagtat
     7921 ggatttaggg aactaaactt aaataaaata attgcaataa cctatgaaga aaaccaaaat
     7981 tctacaaaat cattagattt attaggattt aaatgtacag gaaagtatat caaaaaaatt
     8041 catactggaa atactgtaaa aaatgttcct tgttatcaat atgaattgaa aaaacaatag
     8101 atataatcat ttaatatcta tgctggttat ttagtaatga ttgttttcta aataaaattt
     8161 caataaagtg gttgcctcaa aatatagata atcctgtttt tattgttaaa atactgaaaa
     8221 tgcatttgat tttatacaaa aaagagtgtc tcatgaacta aaaagtttat gagacactct
     8281 ttttgaataa atcgtatttt aattaaaata gtatatatac ttaaaatatt aatttttatt
     8341 agttctctaa atttttaaat ccttctccaa gaacctcatg tacatctgta acaataacaa
     8401 atgcagtagg gtcagttctt ttaactaatc tctttagata aagttcttgt tttttagaaa
     8461 ctacgactaa tagaacttct ttattttctc tagtgtatcc acctttacca tcaagaatag
     8521 taagacctct atcaagttct tcagttatag cttgtcttaa actttcaggc tcatcagaga
     8581 taataaaaaa tgcttttgaa tggtcaaatc cttccattat catatctgct atttttatta
     8641 atacacaaag tgctatacca gagtaaagag cagtttctaa actacgattt actataccag
     8701 aagatataac aaccattcca tctaggcatg acatgaattt tgtagctttt attcctggga
     8761 atttcttact aagtatgagt gcaattaagt ctgttcctcc agtagaagca tctgatttaa
     8821 acattattcc taaacctata ccaactagga ttcctcctgc aatagaagac aataataagt
     8881 cattagtaaa tcttagtttt gataatgggt ctgttaattg aactatagct gaaaatacaa
     8941 ctgttccaaa taaagttttt aatgaatttt ttgtacccat aattctaaaa gcaagtatta
     9001 ctaatgggac acctataatt aacatgatag ctgatactgg aagtcctgta accttgttta
     9061 gtactaatga tagaccacta agtcctccag gagcaattgt atgtggtttt aaaaacatat
     9121 ttataccaat actcattgat atacaaccaa aaaatagtcc aagcatttca agtaaaattg
     9181 gcgacttttg ttttttcata taaacacctc catgaacttg tcacaattta atattataac
     9241 atatttgacc agtttatatt atataaaatt tcttgatata agcaatttca agtatttcat
     9301 tattcttaat atttttgaat ttttatatag tgttttcata tctttactga tgttgttaca
     9361 ctcctatgaa gttttttata tattataatt tttttattct tttataaagt atcccctata
     9421 aagatggttc tatgaaaatt ttctctatat tgtttaacgt atatagagat tttgtgtata
     9481 attataaggt atttaatgga aaaatttatc ttacttttat atgttatcaa aa
//
LOCUS       DAMJYR010000133         9337 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.138, whole genome shotgun sequence.
ACCESSION   DAMJYR010000133 DAMJYR010000000
VERSION     DAMJYR010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9337
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.138"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            125..754
                     /locus_tag="PC471_003142"
     CDS             125..754
                     /locus_tag="PC471_003142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2790250.1"
                     /translation="MNKYLLINPVAERMYGEKFHLIKANLIKKGYIIAECEPQLEYVK
                     KQYKEYTKKTENTMLDCRCPESISLLKRNNLINDFEVPMIEPILVRTCRVLYDKYIKS
                     KDDMLVVTCPCTQLRDFASEKFKDKGNAMFYTWKEFAEQEGVKSLGKIDESPIPLGYF
                     KDTFEKVLEVSSEDEILSEIQKIRNGFEDKYDIVEMFYCKDGCNNGDGL"
     gene            755..1537
                     /locus_tag="PC471_003143"
     CDS             755..1537
                     /locus_tag="PC471_003143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2790251.1"
                     /translation="MVNKFNNKFLRLVKSVLIFFIILLLWKITNYLGIWSDYILPSPE
                     KVYSTFLNMISDGSIFINVYASMKRVLIGFAISTAIGVPLGIFFGIYSGVYEYFKSLI
                     NFLRNTPPLALIPMLILWFGIGEESKIIIIVLASFFPIFTSTLKGIKNCDSKLIEVGR
                     VFEFSKLQIIFKIIIPNAILDIAVGLKLALGYSFRAIIGAELVAASSGLGYLISDGKE
                     MSRTDVVIVGIIVIGLLGIITDYIFSIIVKKVSKGKMVEAYE"
     gene            1530..2408
                     /locus_tag="PC471_003144"
     CDS             1530..2408
                     /locus_tag="PC471_003144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2790252.1"
                     /translation="MNNLVCDSGYIIRNLYKSYKVDNEEHVVLSDISLNIDRNHITVI
                     LGESGCGKTTLLRAIASLEGINSGEIRFINDNKEYRPNVGFVFQESRLMPWLNVSENI
                     AFHNQRKNTILNKVLRKFKHNKRISSKIINDNFRSLETNDNLNKSNKYNRKSNIDVEK
                     YLKMMNLQKFKNSYPNELSGGMAQRVSIARALSFNPDMLLMDEPFSALDYFTRIDMQN
                     EVIRIHKSTNKGVIFVTHDIDEALKIGKKIVVFTDERKVKEFNIEDGYNRDLTSDYYI
                     KLKKDILMTMKSNKGI"
     gene            2441..3400
                     /locus_tag="PC471_003145"
     CDS             2441..3400
                     /locus_tag="PC471_003145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrtA/SsuA/CpmA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HDF2790253.1"
                     /translation="MKLKKLLIGITILTLATASLAGCAKKNNGEKLSEINLTYVKSPL
                     NVPSIIQKQDDLFGKEFKKDNIKVNFHEITTGPEQTQALAAGEIDFLHALGGTSALIA
                     ASNGVELKILNTYSRSPKGFMILTNNNSIKSAADLVGKKVAGPKGTILHQVLISALDK
                     EGLSMDDVEFVNMGIPEASAALSDGSVDAALIAGPAALKAMKSGSKLVANGEGLVDGI
                     IVTAVSTDFAEKHPEIVERFMKVEKETLEYVNNNFDEAMEKVAKEVDLSLEETKELYA
                     WYDFSLDITDKDISSLEDTQDFLIKNKLQEKKVNIKELIYNAK"
     gene            3715..4407
                     /locus_tag="PC471_003146"
     CDS             3715..4407
                     /locus_tag="PC471_003146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011987369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2790254.1"
                     /translation="MSYKVYIVEDDISISTLLKDYIEKYGFEVIVEENFDDIMITFES
                     FKPHLVLLDVNLPKFDGFYWCRCIRQQSNLPIIFISARDGNVDQIRALESGADDYITK
                     PFYYDVVMAKIKSHIRRVYGEYSPKIDERIVELDGLKFYPERLELELNGENLIITKKE
                     GILIECLIKRYPKVVSRDYLLEKIWDDIEFVEENTLNVNVSRIRKRFEELGIDNAVQT
                     VRGLGYKLNKNW"
     gene            4417..5421
                     /locus_tag="PC471_003147"
     CDS             4417..5421
                     /locus_tag="PC471_003147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF2790255.1"
                     /translation="MKLFLKDYKGYIFIYYIGIVMTIIYCNLMKFISFGESLYILLFS
                     SFILLCFLIYKYYKNREVYNLFEKGVHSLEESFLYLGSSFLGRNISVILKQQHKLYQS
                     MIQEHKKIHSEHLTFINQWIHQMKTPISVISLQLQDYEGEEVTENIRKEIDRLNKGLD
                     MAMHFARADNFQKDFIVEKAKLNEIVSSIVNEEKRLFIKSGMIPKIQVDKFTYVYTDV
                     KWMKFIIGQLITNGVKYSKDYSNYLTIKSEENNKYVILSIIDEGIGIVSKDLKRVFDP
                     FFTGENGRKFGESTGMGLYLVKKVCDDLGHKVSIKSEVGKGTEVSIYFNKDVNSLIKV
                     "
     gene            5689..6540
                     /locus_tag="PC471_003148"
     CDS             5689..6540
                     /locus_tag="PC471_003148"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HDF2790256.1"
                     /translation="MSLDVIFILKSVIIAIVEGLTEFIPVSSTGHMILVGNLIDFKGQ
                     FAEMFEVVIQLGAILAVVVLYWKKIKDSVIEFFKFIFTGGKEGKIGFRFGMNVIIGCI
                     PFAIIGVLFYDNIKSLFNLQSVIIGFIVGGILLLVVETLFRKKNHSTDNIDKITPIQA
                     LKVGTLQVLSAWPGMSRSASTIMGGWIAGLNSPTAAEFSFFLAVPAMVASSGKDLFEF
                     DYSIMTPTLWIALVVGFIVAFIVSIIVMEKFVNFLKKKPMRVFAVYRIIMGVVLAVLA
                     FTNIISV"
     gene            6644..7390
                     /locus_tag="PC471_003149"
     CDS             6644..7390
                     /locus_tag="PC471_003149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001637630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2790257.1"
                     /translation="MKILTVNNLSKVYGKKIIFNALNDINFSIEDGEFVGIMGPSGSG
                     KTTLLNMISTIDKPTTGTMELKGKNPLLLRGEELALLRRRELGFVFQDFNLLDTLTIG
                     ENIVLPLTLDKVSVKEQDERLNEVSTILGIKDLLGKRTFEVSGGQAQRTAIARALINN
                     PSILLADEPTGNLDSKSSKVVMELFQKINKENKVTTMMVTHDPLAASYCSRILFIKDG
                     SIYNEIYKGSSREQFYQEIMDVLTLLGGDN"
     gene            7390..9306
                     /locus_tag="PC471_003150"
     CDS             7390..9306
                     /locus_tag="PC471_003150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2790258.1"
                     /translation="MNIKQFAINNISRNIKAYLGYLASIVISSSLLFSFIMFTSHPDL
                     DVSQFPDYLKEGLKMSKIIAYLFLFFFVFYSVSVFLKSRYKEFGILYITGISKRQVMK
                     LIFIENMIINIVSSVIGIIIGLIFAKIFLVIMSTFLELSPLKFYIPFNAILSTLIYFM
                     ILAVLTSIFTSFIVKENQVLKLLKGTKTPKPEPKTSLMLAILSICLFIIAYYNAITST
                     DQYTTYLRLVPVTSLTIVATYLFFSQFSVFFMKKLKLNKRFYRKNTNMLWISNLIYKV
                     KDNARIFFLITITSAVAFTAIGTVYSFWKDVERQINLIYPNTIYYSTMTLHNDPKKPD
                     SKEKDKERMMFIENNLKKENVDYTRTNGEFKTVFPKKSDFNVRIMKESKYLEITETIG
                     VKSISFEDNECISLLSSSLPGNNNVKENIIVGNRGLKVAKQVEECVMPAYYKNMYVVK
                     DNFYDSIESKFIVDRFTAFEVEDSSKTIEICREFENKFNNESGMQPYLFFSKELMIGT
                     GKLMYSTFIFLAIFIGLIFFVTTSSFLYNKFYMDCQVDKKKYEQLNKLGMTYNEIKKA
                     STIEIGIVFLFPYVVAVIHSIFALTALKNSFDIEVASSAVLVMGSLFLVQIVYFFIVR
                     NNYLKEIRLNLVNF"
BASE COUNT         3628 a          879 c         1660 g         3170 t
ORIGIN      
        1 aaatattaaa aaatgtagat aaaaattgtt ttattaagaa aaaattaata tattgacaaa
       61 atttaagtgc tcatttaaac tattaattga taataatatt caatattaaa taataggagg
      121 cgttatgaat aaatatcttc taataaatcc agtggcagaa agaatgtatg gagaaaagtt
      181 tcaccttatt aaagcaaatt tgataaaaaa ggggtacata atagcagaat gtgaaccaca
      241 gttagaatat gtaaaaaagc agtacaaaga gtatacaaaa aagactgaaa atacaatgtt
      301 ggattgtaga tgtccagaaa gtataagtct tttaaaaaga aataatttga taaatgattt
      361 tgaagtacct atgatagaac caatattagt aaggacttgt agggttttat acgataaata
      421 tataaaaagt aaagatgata tgttggtagt aacctgtcca tgtacacaat taagagactt
      481 tgcaagtgaa aagtttaagg ataagggtaa tgctatgttt tatacatgga aagagtttgc
      541 tgaacaagaa ggtgtaaaat cacttggaaa aatagatgaa tcaccaattc cattaggata
      601 tttcaaagat acttttgaaa aagttcttga agtatcaagt gaagacgaaa tattaagtga
      661 aatacaaaaa ataagaaatg gctttgaaga taagtatgac atagtagaaa tgttttattg
      721 taaagatggt tgtaataatg gagatggttt gtaaatggtc aataaattta ataataagtt
      781 tttaaggcta gtaaagagtg ttttgatatt ttttataatt cttttattat ggaaaattac
      841 aaattatctt ggtatttgga gtgattatat attaccatca cctgaaaagg tatatagtac
      901 atttttaaat atgataagtg atggttcaat atttattaat gtatatgcaa gtatgaaaag
      961 agttttgata ggttttgcaa taagtactgc tataggtgtg cctttaggga tattctttgg
     1021 aatttatagt ggagtatacg aatactttaa atctctaata aattttttaa ggaatacacc
     1081 tccccttgct ttaataccaa tgcttatatt atggtttggt ataggagagg aatcaaaaat
     1141 tataattata gttttagcat cctttttccc aatctttaca agcacattga aagggattaa
     1201 aaattgtgac tcaaaactga ttgaagtggg tagagtattt gagttttcaa agcttcagat
     1261 aatatttaaa ataataatac caaatgccat tcttgatatt gctgttgggt taaagttagc
     1321 tcttggatat agctttagag ccattattgg ggcagaactt gtagcagctt catctggtct
     1381 tggatacttg atatctgatg gaaaagaaat gtctagaaca gatgttgtta ttgtaggtat
     1441 aatcgtaatt ggacttttag gaataattac agattacata ttttcgatta ttgtgaagaa
     1501 ggtaagtaag ggaaagatgg tggaagcata tgaataattt agtatgtgac tctggatata
     1561 taattagaaa tttatataaa agttataaag ttgacaatga agaacatgta gttcttagtg
     1621 atatctcttt aaacatagat agaaaccata taacagtaat tttaggtgaa agtggttgtg
     1681 gaaagactac cttgcttaga gcaattgcat cacttgaagg tataaatagt ggtgaaatta
     1741 ggtttattaa tgataataaa gagtatagac ctaatgttgg ttttgttttt caggagagca
     1801 gattaatgcc ttggttaaac gtaagtgaaa atatagcttt tcataatcaa agaaaaaata
     1861 ctattttaaa caaggtgctt agaaaattta aacacaataa aagaattagc agtaaaatta
     1921 taaatgataa ttttagaagc ttagaaacaa atgataattt gaacaaatca aataaatata
     1981 ataggaaaag taatattgat gtagaaaaat atcttaagat gatgaacttg cagaaattta
     2041 aaaactctta tccaaatgaa ttgtcaggag gtatggcgca gagagtaagc atagctagag
     2101 ctctttcttt caatccagat atgttgctta tggacgagcc tttttcggca ttagattatt
     2161 ttacaaggat tgatatgcaa aatgaagtta taagaataca taagtcaact aataaaggcg
     2221 tgatatttgt aactcatgat attgatgaag cgttaaagat aggcaagaaa atagttgttt
     2281 tcacagacga aagaaaagta aaagaattta atatagaaga tggttataat agagatttaa
     2341 cttcagatta ctatattaaa ttaaaaaaag atattttaat gacaatgaaa tcaaataaag
     2401 gcatttaaag aaattaataa tattttaagg agtaaagtaa atgaaattaa aaaagctatt
     2461 gataggtata acaatattaa cacttgcaac agcatcttta gctggatgtg ctaaaaaaaa
     2521 taatggagaa aaactttcag aaataaattt aacttatgta aaatcaccat taaatgttcc
     2581 ttctataatt caaaaacagg atgatttatt tggaaaggaa tttaaaaagg ataatataaa
     2641 agttaatttc cacgaaatta ctacaggtcc agagcaaaca caagctttag ctgctggaga
     2701 aatagacttc ttacatgcac taggtggtac atcagcttta atagctgcat ctaatggagt
     2761 agagttaaag atattaaaca cttatagcag gtcaccaaaa ggatttatga ttttgactaa
     2821 taataattca ataaaatcag cagcagattt agtaggtaag aaggtggcag gcccaaaagg
     2881 gactatatta catcaggtat tgatttcagc acttgataaa gaaggattaa gtatggatga
     2941 tgtggaattt gttaatatgg gtattccaga ggcatcagca gctctttctg atggcagcgt
     3001 agatgctgca ttgattgctg gtccagcagc tcttaaagct atgaagtcag gtagtaaact
     3061 tgtagcaaat ggtgaaggtt tagtagatgg tataatagta actgctgtta gcacagactt
     3121 tgcagagaaa catccagaaa tagttgaaag atttatgaag gttgaaaagg aaactttaga
     3181 atatgtaaat aataattttg atgaagctat ggaaaaagta gctaaagaag ttgatttaag
     3241 tcttgaagaa actaaagaat tatatgcttg gtatgatttt agtttagata taacagataa
     3301 agacatatct tcgcttgaag atacacaaga cttcttaata aaaaataagt tacaagagaa
     3361 aaaagtgaac ataaaagaat taatctataa tgctaagtag aaactatgat aaaataatac
     3421 taagtaagaa atatagtaaa agtttaagtt accttattaa ggtaactttt tctattttga
     3481 attagtaatt tcaatttatt ttagaatgat tagttgataa taatgattaa ttaatcataa
     3541 aaagatactt ttttatagag ttttattaag atgtctaata atatggttat acttatgaag
     3601 tcagtaattg tgaagttagt aatatgatta aatttatata ttgtaaaaag attataatat
     3661 attaaaattg gactgttaat atgatgagta aataggtata ttaggaggaa ttatatgagt
     3721 tataaagtat atattgtgga agatgatata tcaataagta cattacttaa agattatatt
     3781 gagaaatatg gatttgaagt gattgttgaa gaaaactttg atgatataat gattacattt
     3841 gaaagtttta agccacactt agttctatta gatgtaaatt tacctaaatt tgatggtttc
     3901 tactggtgta gatgtattag acaacagtcg aatttaccta taatttttat atcagctaga
     3961 gatggtaatg tagaccaaat tagagcttta gaaagtggag cagatgatta tataacaaaa
     4021 cctttttact atgatgtagt aatggcaaaa ataaagagtc atataagaag ggtatatggt
     4081 gaatattcac ctaaaataga cgaaagaata gtggagctag atggattaaa gttttatcca
     4141 gagaggttag aactagaatt aaatggtgag aatctaatta taactaaaaa agaaggtata
     4201 ctgattgaat gtcttatcaa gagataccca aaagtagtaa gtagagatta tcttttagaa
     4261 aagatatggg atgatattga gtttgtagag gaaaatactt taaatgtaaa tgttagcagg
     4321 ataaggaaaa gatttgagga attaggtata gataatgctg ttcagactgt ccgagggtta
     4381 ggatataaat taaataagaa ttggtaagga gaaagtatga agttattttt aaaagattat
     4441 aaaggttata tttttatata ttatataggc atagttatga caattattta ctgtaatctt
     4501 atgaagttta ttagttttgg agagagttta tatattcttt tatttagttc ttttatattg
     4561 ttatgttttt taatatataa gtattataaa aatagagaag tatataatct gtttgaaaaa
     4621 ggagtacatt ctttggagga gtcatttttg tatttaggaa gctcattctt ggggagaaat
     4681 atatcagtta ttttaaaaca acaacataaa ttgtatcaat caatgataca agagcataaa
     4741 aagatacata gtgaacattt aactttcatc aaccaatgga ttcatcagat gaaaacacca
     4801 atatcagtta taagccttca gcttcaagat tatgagggag aagaagtaac tgaaaatata
     4861 agaaaagaaa tagatagatt aaataaaggg ttagatatgg ctatgcattt tgcaagggca
     4921 gataattttc agaaagactt tatagttgaa aaagccaaat taaatgaaat tgtatctagt
     4981 attgtaaatg aagaaaaaag gttatttata aaaagtggaa tgattcctaa gattcaagta
     5041 gacaaattta cttatgttta tacagatgta aaatggatga aatttataat aggtcaatta
     5101 attacaaatg gagtgaaata ttctaaagat tatagtaact acctaactat aaaatctgaa
     5161 gaaaataata aatatgtaat tttaagtata atagatgaag gaattggaat tgtttctaaa
     5221 gatttaaaaa gggtatttga cccatttttt acaggagaaa atggtcgtaa gtttggagaa
     5281 tctacaggta tgggtcttta tctagtaaaa aaggtatgcg atgatttagg tcataaagta
     5341 agtataaaat cagaagtagg aaaaggtaca gaagtttcaa tctactttaa taaagatgta
     5401 aatagtttaa taaaggtgta aatagtttga taaagatgta aattatctgt tacaataggg
     5461 tttttagtta attgtacttt tttattatac aatttattaa atgaaatatt catagttaaa
     5521 taatcaaaat ttaagagcgt ttatttgcaa accttacata gatgtaaggt ttgtgtaatg
     5581 tttgcagata gttaaatttt ctaatctaaa ttacaattaa tataagaaat aacataagag
     5641 aaataacata aaaaaagaaa ttttgtaaaa aaggaaggat gtttattgat gagtttagat
     5701 gttattttta tattgaaatc agtcataata gctatagtag aggggcttac agaatttatt
     5761 ccagtttcat ctacaggtca tatgattttg gttggtaatt taatagattt taaggggcaa
     5821 tttgctgaaa tgtttgaggt tgtaatacaa cttggggcta tattagcagt agtagtactt
     5881 tattggaaaa agataaaaga tagcgttata gaatttttta aatttatatt tactggtgga
     5941 aaagaaggaa agattggttt tagatttgga atgaatgtaa ttataggttg tataccattt
     6001 gcgattatag gagttctatt ttacgataat ataaaatcat tgtttaattt acaatctgta
     6061 attataggat ttatagtggg tgggatttta ttattagtag tagaaacttt gtttagaaaa
     6121 aagaatcatt ccacagataa tatagataaa attactccaa tacaagcttt aaaggttggg
     6181 acattacaag ttttgtctgc atggccaggt atgtcaagga gtgcatctac tattatgggt
     6241 ggatggatag caggacttaa ttctcctaca gcagctgagt tttctttctt tttagcagta
     6301 ccagctatgg tggcttcttc aggaaaagat ttatttgaat ttgattattc aataatgaca
     6361 ccaacattgt ggattgcttt ggtagttggg tttatagtag cttttattgt atcaataata
     6421 gttatggaaa aattcgtgaa ttttttaaag aaaaaaccaa tgagagtgtt tgctgtatac
     6481 agaataataa tgggtgtagt acttgcagta ttagctttta ctaacataat tagtgtatag
     6541 aaaaaataga tttatcatct ataaaaatta aactaagata aattataatt aacaattata
     6601 tatatgttat ttcaaaaatt aaattaggaa ggatagttaa ataatgaaaa ttttaactgt
     6661 aaataattta agtaaagtat atggtaaaaa aataattttc aacgcattaa atgacataaa
     6721 ttttagtata gaagatggag aatttgtagg aattatgggt ccatctggta gtggaaaaac
     6781 tactctttta aatatgattt ctactattga caagccaact acaggaacta tggaattaaa
     6841 gggcaagaat cctttattgt taagaggtga ggaacttgca ttacttagaa gaagagagtt
     6901 aggttttgta tttcaggatt ttaatttatt ggatacatta actataggag aaaatatagt
     6961 attgccattg acattagata aggtcagtgt aaaagagcaa gatgagagat tgaatgaagt
     7021 atctacaata ttaggtataa aagatttatt aggaaaaaga acttttgaag tatcaggtgg
     7081 tcaagctcag aggactgcta ttgctagagc attgattaac aatccatcaa tacttttagc
     7141 agatgaacca acaggtaatt tagattcaaa gtcatcaaag gttgtaatgg agctatttca
     7201 aaagataaat aaagaaaata aagttactac tatgatggta actcatgacc cattagcagc
     7261 gagctactgt agtagaatct tattcataaa agatgggtct atatataatg aaatatataa
     7321 gggtagtagt agagagcagt tttatcaaga gataatggat gtacttacat tattgggagg
     7381 cgataactaa tgaatataaa acagtttgct attaacaata tttcaagaaa tattaaagcg
     7441 tatttaggat acttagcaag tattgttata tcatcttcac tgctattttc ttttattatg
     7501 tttacaagtc atccagattt agatgtatct caatttcctg attatttaaa agaaggtctc
     7561 aagatgagta agataattgc atatctattc ttattcttct ttgtgtttta ttcagttagt
     7621 gtgtttctta aaagtagata taaagaattt ggaatacttt atattacagg tatatcaaag
     7681 cgccaagtaa tgaaattaat ctttatagaa aatatgataa taaatatagt atcttctgta
     7741 attggaataa taataggctt aatctttgca aagatatttt tagtgattat gtctactttt
     7801 ttagaattat caccattaaa attctatata ccatttaatg cgatattgtc tactttaatc
     7861 tattttatga ttttagctgt attaacatca atatttactt cttttatagt taaagaaaat
     7921 caggtgttga agttacttaa aggtacaaaa actccaaaac cagagcctaa aacatcacta
     7981 atgcttgcta tattaagtat ttgtttgttt ataatagcgt attataatgc tattacctca
     8041 accgaccaat atacaacata tctgcgctta gttccagtta catcccttac tatagtagca
     8101 acttacttgt tcttttctca atttagtgta ttttttatga agaagctaaa actaaacaag
     8161 agattttata gaaaaaatac aaatatgctt tggatttcga acttgattta taaggtaaag
     8221 gataatgcta gaatattttt cttgataact ataacttctg cagtagcatt tactgctata
     8281 ggtacagttt attccttttg gaaagatgta gagcgtcaaa ttaatttaat atatcctaac
     8341 acaatatatt attcaacaat gacattgcat aatgacccta aaaagcctga tagcaaagaa
     8401 aaagacaagg aaagaatgat gtttatagaa aataatctaa aaaaagagaa tgtagactat
     8461 acaaggacta atggtgaatt taaaactgta tttcccaaga aaagtgattt taatgtcaga
     8521 attatgaaag aatctaagta cttagaaatt acagaaacta taggggttaa gtctataagt
     8581 tttgaagata atgaatgtat atcattatta tcatcaagtt taccaggaaa taataacgtc
     8641 aaagaaaata ttattgtagg gaataggggt ttaaaggtgg caaaacaagt ggaagaatgt
     8701 gttatgccag catattacaa aaatatgtat gtggtaaagg ataattttta tgatagtata
     8761 gaatcaaaat ttatagtgga tagatttact gcatttgaag tagaggattc aagtaaaact
     8821 atagagatat gcagagaatt tgaaaataaa tttaataatg agtcagggat gcaaccatat
     8881 ttgtttttct ctaaagaatt aatgatagga actgggaaat taatgtattc tacatttata
     8941 tttttagcaa tatttatagg tttaattttc tttgttacta caagtagttt tttatataat
     9001 aaattttata tggattgcca agttgataag aaaaagtatg aacagttaaa taaattgggt
     9061 atgacatata atgagattaa aaaagcttca acaattgaga ttggaatagt atttttgttt
     9121 ccttatgtag tggctgttat acattcaata tttgcactta ctgcacttaa aaattctttt
     9181 gatatagaag tagcatcatc agctgttttg gtaatgggaa gcttgttctt ggttcaaata
     9241 gtttattttt ttattgtaag aaataattat ttaaaagaga taaggttaaa tttagtcaat
     9301 ttttagttac tattattaaa tgataataaa aagggtt
//
LOCUS       DAMJYR010000134         9262 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.139, whole genome shotgun sequence.
ACCESSION   DAMJYR010000134 DAMJYR010000000
VERSION     DAMJYR010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9262)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9262)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9262
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.139"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            286..1302
                     /gene="gap"
                     /locus_tag="PC471_003151"
     CDS             286..1302
                     /gene="gap"
                     /locus_tag="PC471_003151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011948756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HDF2790259.1"
                     /translation="MKIKVGMNGFGRIGRAVLRIAQEELGDNIEIVAINARATTESLA
                     HLFTYDSCYGTFRGEVEAKDEDTLVVNSKEIKILRYNDPEELPWKELGVDIVIESTGL
                     FTQREKAEKHIKAGAKKVIITAPGKNEDITIVIGVNEKEYDNEKHNIISNASCTTNCL
                     APFAKVLDEKFGIVKGLMTTVHSYTNDQRILDKSHKDLRRARAAAESIIPTTTGAAKA
                     VSRVLPQLEGKLNGFSLRVPTPTVSLVDLVCELKENVTSEQVNSVLKDAAEGELKGVL
                     GYCDKPLVSIDYKGDSRSSIIDALSTMVIEDNMVKVVSWYDNEWGYSSRTVDLVKYVA
                     EKLK"
     gene            1697..2866
                     /locus_tag="PC471_003152"
     CDS             1697..2866
                     /locus_tag="PC471_003152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2790260.1"
                     /translation="MEKLTDRRQVKAVSMETFIFLILLVVGFGYVGSIMGAGMMFKVI
                     MSTAHALLLETVFLIMAMAVLAGALSALLSEFGVIALINKIFAVFMKPLYGLPGASIA
                     GAITTYLSDNPAIIPFAKDKTFTQYFKQYQVPALCNLGTAFGMGLILTTFMISQGTEY
                     VLPALIGNLGAIIGSVISVRIMLTFTKKFYKYSPEEDKATGTLEKKDEFREIREGNVF
                     QRALDAILEGGKMGVDMGMAIIPGVLVVCTLVMLLTFGPSTDPVTGQEVYTGAAYEGI
                     KLLPVIGDKLGFILEPLFGFTSPEAIAFPITSLGAVGAAMSLVPEFIKSGAITPNDIA
                     VFTAMGMCWSGYLSTHIGMMDALNARQLAGKAILSHTIGGLCAGAAAHFIFTLVG"
     gene            3246..7499
                     /locus_tag="PC471_003153"
     CDS             3246..7499
                     /locus_tag="PC471_003153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase"
                     /protein_id="HDF2790261.1"
                     /translation="MDELFHIGIDVGSTTVKVVVLNNSNNIVHKEYRRHYSDVKKSVK
                     EVLNGIYEKLGDINTTIIITGSGGIGISKKLGVKFVQEVISSTKSIEYFHPETDVVIE
                     LGGEDAKITYLSGGIDQRMNGICAGGTGAFIDQMASLLKTDASGLNELAKGYNVIYPI
                     ASRCGVFAKTDIQPLINDGANQTDIAMSIFNAVVVQTVSVLSCGRKIEGNVAFLGGPL
                     YFLSELREAFKRVLDLKDENIIFPENAQLYIAIGAGLLSVDEDNIQLKSLIEKLDSIK
                     NIEDGEVNLLEPLFKDKNEYEEFSRRHEKEKINYVDINSIKSNCYLGIDAGSTTTKAA
                     LIDEDGRLVYSYYNSNEGNPLKTTIKVINEVYDILPKNIKILSSTVTGYGEGLIKKAL
                     KIDNGEIETIAHYKAAKFFNKDVDFILDIGGQDMKCLKIKDGVIDNIILNEACSSGCG
                     SFLETFASSLSMTIEEFAHEGIYSQNPVDLGSRCTVFMNSRVKQAQKEGASVGDISAG
                     LSYSVIKNALFKVIKIRDLNEIGNNIVVQGGTFYNDLVLRSFEKLIGKNVIRPNISGI
                     MGAFGSALIAKEKYESGYKTSLLSKEELNNIKLEASVTRCKGCSNHCLLTINKFSDNE
                     IFISGNRCEKGEAIYGDKKIGLEKKKPINLFKYKYNRIFRYKPLEKEEAKNGEIGIPR
                     VLNMYEDYPFWFTFFNAMGFRVVLSDRSSKQLYETGITSIASETVCYPGKLVHGHIEN
                     LIAKGIKNIFYPSVTNENKEDSNADNYYNCPVVISYSEVIKNNVENIRNKNINYINPF
                     ISLNDKEKLKKRLYDELSRHFSDIKITKEEVNHAVDKATDEQNLFKMEIQAAGERALK
                     EIKERNMKGIVLCGRPYHIDPEINHGMPELINSLDMAVLTEDSICHLANVQRPLRVVD
                     QWVYHSRLYKAASFVRDKNYLELVQLNSFGCGLDAVTTDQVQEILNEKSKIYTIIKID
                     EGNNLGAAKIRMRSLKAAMFERESKNIDVKNIKVNRIQYAKNNQITNKHTILAPQMSP
                     IHFQFLEKAVNLSGYNIEILKDTDSSVIEEGLRYVNNDACYPAIIVVGQLITALKSGK
                     YDLNNTSVTITQTGGGCRATNYIGFLRKAIYDAGFKDVPVIALSVNGIEDSGIMDNIS
                     LKLINRLFMSVVYGDLLMKVLYRVRPYEKVTGSANALYEKWVDICKSSLVKAKISVFT
                     RNIKDIVKEFDNLEILDIKKPKVGLVGEILVKFHPIANNNLVDILEREGAEAVVPDLT
                     NFFLSCAFNTIYKHTHLEGSRKSRMIGEAFIYITGIYQRVYKKALDKSERFYAPANIK
                     NVAKSTEPVVSLGNQTGEGWLLTGEMVELLNEGVENIICMQPFGCLPNHIIGKGSIKE
                     LKRLYKNANIIPIDYDPSASEVNQLNRIKLMLSKAFKNI"
     gene            7648..8142
                     /locus_tag="PC471_003154"
     CDS             7648..8142
                     /locus_tag="PC471_003154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HDF2790262.1"
                     /translation="MSLVYDVYKSKLGNLYILSEDGFIVSIYIGDEKFNNLKNECIKK
                     GIDIKRSKEDLKEAISQIDDYFNGKRKVFNLNIRIEGTEFQKSVYREMLKIPYGETLC
                     YSDIARNINNPKSVRAIGQASKSNKIPIIIPCHRVVGKKDIGGYMGNHSDLKEILLNL
                     ERQS"
     gene            8258..8635
                     /locus_tag="PC471_003155"
     CDS             8258..8635
                     /locus_tag="PC471_003155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monooxygenase"
                     /protein_id="HDF2790263.1"
                     /translation="MCDKVIASKYHEKGFNCAESVIKAYNEEFNTDIPVCLGSGLGSG
                     CGVASLCGAVNASNIIIGYVKGRNHEDESTKAKVYAKDLTTTVRKEYGSELCIDLKKD
                     LVACREIMDFAYDSLKETLKKEL"
     gene            8841..>9262
                     /locus_tag="PC471_003156"
     CDS             8841..>9262
                     /locus_tag="PC471_003156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufBD protein"
                     /protein_id="HDF2790264.1"
                     /translation="MKNLNLLINKLYSKNHNEAYKTFLFLENESLKSNITYCFFDSFL
                     EMIDNENSYIRTRGLLLISANAQWDIDNKIEINIDSILSHIVDKKPSVSRMFIKSIPN
                     ITKYKENLINRIKMELSNADISIYNNNMKPLVEKDINDT"
BASE COUNT         3757 a          962 c         1664 g         2879 t
ORIGIN      
        1 gaagatatat tatttttaaa aataagtata gtttaaaaat tcaaaaaaaa gatggcaaaa
       61 tatgtcccaa gggacattga gtaaaataaa tgatttcagc ttatttacac taaaaagtac
      121 atactaaatg aggggtaaaa taaagaaaaa tgatatacta attgtctttt gataataaaa
      181 aaacatggaa tttgattatt taatcaagaa aaagtattga acaaatgatt tatttagtgt
      241 acaataatca tgtaaaaaca tagtaatgga gggagacatt tcataatgaa aattaaggta
      301 ggaatgaacg gatttggaag aataggaaga gctgtattaa gaatagctca agaagaatta
      361 ggagataata tagaaattgt agccataaat gcaagagcaa caacagaatc tttggcacat
      421 ttatttacat atgattcatg ctatggaact tttagaggtg aagtagaggc aaaggatgaa
      481 gacactctag tagtaaactc taaagagata aaaatattaa gatataatga cccagaagaa
      541 ttaccatgga aagaactggg ggttgatata gtaatagaat caacaggatt atttacacaa
      601 agagaaaagg cagaaaagca tattaaagca ggagcaaaaa aagtaattat aacagctcca
      661 ggaaaaaatg aagatataac tatagttatc ggagttaatg agaaagaata tgataatgaa
      721 aagcacaata taatttcaaa tgcatcttgt actacaaatt gtctagctcc ttttgctaaa
      781 gtattggacg aaaaatttgg tatagtaaaa gggttaatga ctacagtaca ttcatatact
      841 aacgatcaaa gaatattaga taaaagccat aaagatttga gaagagcaag agcagcggct
      901 gaatctataa tacctaccac tacaggagca gcaaaagctg tgtcaagagt actaccacaa
      961 ttagaaggaa aattaaatgg tttttcactt agagtaccaa caccaacagt ttcacttgtt
     1021 gatttagtgt gcgaacttaa agaaaacgtt acatctgagc aagttaactc tgtgttaaaa
     1081 gatgctgctg aaggagaatt aaaaggtgtt ttaggatatt gtgacaagcc acttgtttct
     1141 atagattata aaggagattc aagatcatct attatagacg ctctatccac aatggttata
     1201 gaagacaata tggttaaggt agtttcttgg tatgataatg aatggggata ttcttcaaga
     1261 actgttgact tagtaaaata tgtagctgaa aaattaaaat aatatatatt tcttaaagca
     1321 aattttaaaa aaatttttta aaaaaattat ataaaactaa aaaaaaagat tatgaaaaat
     1381 attgaaggaa aaattttttc ataatctttt ttctttcatg caaaaagata caagttacaa
     1441 cttatatttg catattaggt cgttagagaa gtatatatat atttagtaag gaatcatact
     1501 ttttataaaa tgaaagataa gatgtcgaaa acttgcataa atctgaaaaa tttttattat
     1561 taaaatactg atttccattt taaaaatgat aaaaaaatga caaataaagt tgcaaatagt
     1621 aatacttata agtataatta aaaaagagaa aaggttttgt taaactttat aacaaaaaaa
     1681 gaagggatga ttcaaaatgg agaagttaac agacaggaga caggttaagg cagtaagcat
     1741 ggaaaccttc attttcctaa tattactagt agttggtttt gggtatgttg gtagtattat
     1801 gggagcagga atgatgttca aagtaataat gagtacagca catgctttac tattggagac
     1861 agttttctta attatggcta tggcagttct tgcaggcgca ttaagcgcac tactttcaga
     1921 gtttggagta atagcgttaa ttaataaaat tttcgctgta tttatgaagc ctttatatgg
     1981 actacctggt gctagtatag caggagctat aacaacttat ttatcagata atccagctat
     2041 tatacctttt gctaaagata aaacattcac acagtatttt aaacaatacc aagttccagc
     2101 attatgtaac ttaggaacag cctttggtat gggacttatc ttaacaacat ttatgatttc
     2161 tcaaggtaca gaatatgtac ttccagcatt aattggtaat ttaggtgcaa ttataggtag
     2221 tgtcataagt gttagaataa tgttgacatt tactaagaag ttttataaat acagtccgga
     2281 agaagataaa gcaacaggaa cattagagaa aaaggatgaa tttagagaaa tcagagaagg
     2341 aaatgtattt caaagagctt tagatgcaat tcttgaaggt ggtaaaatgg gggtagatat
     2401 gggaatggct ataattcctg gagtcctagt agtatgtaca ttggtgatgt tattaacttt
     2461 tggaccatct acagaccctg ttacaggtca agaagtttat acaggagctg catatgaagg
     2521 aataaaatta cttccagtta ttggagataa acttggattt atattagaac cattatttgg
     2581 atttacatca ccagaggcaa tagcattccc tataacttca ttgggagctg ttggcgcagc
     2641 aatgtctcta gttccagagt ttataaagtc aggagctata actcctaatg atatagcagt
     2701 atttacagct atgggtatgt gttggagtgg atatttaagt acacatattg gtatgatgga
     2761 tgcattaaat gctagacagt tggcaggtaa agctattctt tctcatacaa taggtggttt
     2821 atgtgcagga gcagctgctc actttatatt tacactcgta ggataaatgt aatcagctaa
     2881 gataaaaaat aatatacaaa aaatggttgt tgatatgtaa taagaattta catatcaaca
     2941 accatttttt tctgactaaa gataatttat aatacataaa cattaagtgg atattagagt
     3001 taagttatat ttatgtaaaa agtaacaaaa atctgtagaa atttatgaca aaatttagta
     3061 cactaactat atgttcttat ttaaaattta aggttagaat taggaattac ctagtagagt
     3121 aagctttgat taataaaaga atcataaaat aaaaatatta gtcataaaga ttataaataa
     3181 cttacttaaa ataaagccat agaacttaca aaaattaaaa aagcatataa atatggaggg
     3241 gtacaatgga tgaattattt catataggaa ttgatgtagg ttctacaacg gtgaaggttg
     3301 tggtacttaa taatagtaat aatatagttc ataaagaata tagaagacat tattctgatg
     3361 taaaaaaatc agttaaagaa gtactaaatg gtatatatga aaaattggga gatataaata
     3421 ctaccattat aataactggt tcaggtggaa ttggaatatc aaaaaaatta ggtgtaaaat
     3481 ttgttcagga agttatatct agtacaaaat caatagagta ttttcatcct gagactgatg
     3541 ttgtcataga acttgggggt gaagatgcaa agataacata tctaagtgga ggaatagacc
     3601 aaagaatgaa tggaatatgt gcaggtggca ctggagcatt catagaccag atggcatcac
     3661 tcttaaagac agatgccagt ggattaaatg aacttgctaa agggtataat gttatatatc
     3721 ccatagcttc aagatgtggt gtttttgcta aaacagatat tcaacctctt ataaatgatg
     3781 gagccaatca gactgatata gcaatgagta ttttcaatgc agttgtcgta caaactgtga
     3841 gtgtcctttc ttgtggaaga aaaatagaag gaaatgttgc gtttttgggt ggtccattgt
     3901 actttttatc tgaacttaga gaagcattta aaagagtatt agatttaaaa gatgaaaaca
     3961 taatcttccc agaaaatgca caactttata ttgctattgg agcaggatta ttatcagtag
     4021 atgaagataa tatacaacta aaatctctaa tagaaaaatt ggatagtata aagaatattg
     4081 aagatggaga agtaaattta ttagaacctt tatttaaaga taaaaatgag tatgaagaat
     4141 tttcaagaag acatgaaaaa gaaaaaataa attatgtgga cataaattca ataaaaagca
     4201 attgttattt aggaattgat gcaggctcaa ctacaactaa agcagcactt attgatgagg
     4261 atggaagact tgtatattct tattataata gcaatgaagg aaatccatta aaaacaacca
     4321 ttaaagttat aaatgaagta tatgatatat taccaaaaaa tattaaaata ttgagttcaa
     4381 ctgtaactgg atatggagaa ggtcttatta aaaaagctct taaaatcgat aatggagaga
     4441 tagagactat agcacattac aaagctgcta aatttttcaa caaagatgta gattttatac
     4501 ttgatattgg tggtcaagat atgaagtgcc taaaaataaa agatggagta attgataata
     4561 taatattaaa tgaggcttgt tcatcaggtt gtggctcatt tttagagaca tttgcaagtt
     4621 ctttatctat gacaatagaa gaatttgcgc atgaaggtat ttattctcaa aatcctgtag
     4681 atttaggttc aagatgtact gtatttatga attctagagt aaaacaagct caaaaagaag
     4741 gtgcaagtgt tggagatata tcagcagggc tatcttattc agtaataaaa aatgctttat
     4801 ttaaagtaat aaagattaga gatttaaatg agattggtaa taatattgtt gtacaaggtg
     4861 gaacctttta taatgatttg gtgcttagaa gctttgaaaa actaattgga aaaaatgtaa
     4921 ttagacctaa tatttcagga attatgggag cttttggttc agcacttata gctaaagaaa
     4981 aatatgaaag tggatacaaa acatccttgt tatctaagga agagttgaat aatatcaagc
     5041 tagaagcaag tgtaactaga tgtaaaggtt gttcaaatca ttgtcttctt acaataaata
     5101 aattttctga taatgaaatt tttatttcag gtaatagatg tgagaagggt gaagctattt
     5161 atggtgataa aaaaatagga ttagaaaaga aaaaaccaat aaatttattt aagtacaaat
     5221 acaatagaat ttttagatac aaaccattgg aaaaagaaga agcaaaaaat ggggaaatag
     5281 ggataccaag agtattaaat atgtatgaag attatccatt ttggtttact tttttcaatg
     5341 ctatgggctt tagagttgta ctttcagata ggtcatcaaa acagttatat gaaactggta
     5401 taactagtat tgcatctgaa acagtttgtt atccaggaaa attagttcat ggacatatag
     5461 aaaatttaat tgcaaaaggt ataaaaaata tattttatcc aagtgtaaca aatgaaaata
     5521 aagaggattc aaatgcagat aattattata attgtcctgt tgtaatatca tattctgaag
     5581 taataaaaaa caatgtagag aatataagaa ataagaacat aaattatata aatccattta
     5641 taagtctaaa tgataaggaa aaattgaaga aaagactcta tgatgaatta tcaagacatt
     5701 tttctgatat aaaaataact aaagaagaag ttaatcatgc agttgataaa gctactgatg
     5761 aacagaattt atttaaaatg gagatacaag cagcaggtga aagagcactt aaagaaataa
     5821 aagaaagaaa tatgaagggg attgtgttat gtggaagacc atatcatata gacccagaaa
     5881 taaatcatgg aatgccagag cttataaatt cattagatat ggcagtgctt actgaagact
     5941 ctatatgtca tttagccaat gtacaaagac cacttagagt tgtagaccaa tgggtatatc
     6001 attcaagatt gtataaagca gctagttttg taagagataa aaactatttg gaacttgttc
     6061 aacttaattc atttggctgt ggattagatg cagtaactac agaccaggtt caagaaatac
     6121 ttaatgaaaa atcgaagata tatactatta taaagattga cgaaggcaac aatttaggag
     6181 cagctaagat aaggatgagg tccttaaaag cagctatgtt tgagagagaa agcaaaaata
     6241 tagatgtaaa aaatataaag gtaaacagga tacagtatgc taaaaataat cagataacga
     6301 acaaacatac tatattagca ccacaaatgt ctccaataca tttccaattc cttgaaaaag
     6361 ctgttaactt aagtggttat aatatagaaa ttctaaaaga tactgatagt agtgtaattg
     6421 aagaagggtt aagatatgta aataatgatg catgttatcc agctataata gttgtaggtc
     6481 agctaataac agcattaaaa tctggcaaat atgatttaaa taacacttct gttactataa
     6541 ctcagactgg tggaggttgt agagctacaa attatattgg attcttaaga aaggctatat
     6601 acgatgctgg atttaaagat gttcctgtca ttgcacttag tgtaaatgga atagaagata
     6661 gtggtataat ggataatata tcactaaaat taatcaatag actctttatg tctgttgttt
     6721 atggtgattt gctgatgaaa gtactatata gagttagacc atatgaaaaa gttactggaa
     6781 gtgcaaatgc tctatatgaa aaatgggttg atatatgcaa atcttctctt gttaaagcaa
     6841 aaatttctgt atttactaga aatataaaag atatagtaaa agaatttgat aatttagaga
     6901 tactagatat caaaaaacca aaagtaggtt tggttggaga aatattggtg aaattccatc
     6961 caattgcaaa taataattta gtagatatat tagagcgaga aggtgctgaa gcagtcgtgc
     7021 cagaccttac aaatttcttc ttaagttgtg cctttaatac tatatacaaa cacactcatt
     7081 tagaaggaag tagaaagagt agaatgatag gagaggcatt tatatacata actggaatat
     7141 atcaaagagt atataaaaaa gcacttgata agagtgagag attttatgct ccagccaata
     7201 taaaaaatgt tgctaaaagt acagaaccag tagtgtcatt aggtaatcaa actggagaag
     7261 gttggctttt aacaggtgaa atggttgaat tattaaatga aggtgtagaa aacattattt
     7321 gtatgcaacc ttttggatgt ttacctaatc acataattgg taagggttct ataaaagaat
     7381 taaagaggtt gtataaaaat gcaaatataa tacctattga ttatgaccct tctgcaagtg
     7441 aggtaaatca acttaacagg ataaaactta tgctttctaa ggcttttaaa aatatataag
     7501 atttatattt tgatatgtta aaagttaatg aatttaaaaa tatactctat attaagtatc
     7561 tataaaaaag agttatccta ttttttaggg taactcttta ttagttaaaa aattatatgt
     7621 atactgatat taaagggggg aagtaatatg agtttagtat atgatgttta taaatcaaaa
     7681 ttaggaaatt tgtacatatt aagtgaagat ggttttatag ttagtatata cataggagat
     7741 gaaaaattta ataatttaaa aaatgaatgt attaaaaaag gaattgatat aaagagaagt
     7801 aaggaagatt taaaggaagc aataagccag atagatgatt actttaatgg aaaaagaaaa
     7861 gtctttaatt taaatattcg tattgaaggg actgaatttc aaaaatcagt ttatagggaa
     7921 atgttaaaaa taccttatgg agaaacattg tgttattcag atatagcaag aaatataaac
     7981 aatccaaaat cagtaagagc aataggacaa gcaagtaaaa gtaataaaat tccaataata
     8041 ataccttgtc atagagtagt tggcaaaaaa gatattggtg gatatatggg gaatcattct
     8101 gatttgaaag agatactatt aaatttagaa agacagagtt aacttagaaa gaaatgtttt
     8161 tattgttaat atttgatata ataatagtaa aaagaagtat tttttacaaa aacaatgaag
     8221 aatttatttt aatttacaaa aatgaggggg aattttaatg tgtgataaag ttatagcatc
     8281 aaaatatcat gagaagggat ttaattgtgc agaatcagtt ataaaagcat ataatgagga
     8341 atttaataca gatataccag tttgtttagg aagtggactg ggttcagggt gtggggttgc
     8401 tagtttatgt ggtgcagtaa atgcatcaaa tataataatt ggatatgtaa aaggcagaaa
     8461 ccacgaagat gaaagtacta aagctaaagt atatgctaaa gatttaacta caacagtaag
     8521 aaaagagtat ggttcagagt tatgtataga cctaaaaaaa gatttagttg cttgtagaga
     8581 aataatggat ttcgcatatg atagtctaaa ggaaacttta aaaaaagaat tataagaaaa
     8641 taagctatca aatataaatt atttgatagc ttatttttat agccgatact aaataaatca
     8701 aatatatttt agaattatta tttgtaaata aacagcaaat aattttaggc tttttattta
     8761 atatagtttt tatttatatt tatttttgaa tatgttatac tttgtttaaa taaatatttg
     8821 taatgaggga gatgagaaga ttgaagaatc tcaatttact tattaataaa ttatattcaa
     8881 aaaatcataa tgaagcatat aaaacttttt tattcttaga aaatgaaagt ttaaaatcaa
     8941 atattacata ttgttttttt gactcattcc tagaaatgat tgataacgaa aactcatata
     9001 ttcgaacaag gggattgtta ttaatctctg caaatgctca gtgggatatt gataataaaa
     9061 ttgaaataaa tatagatagt atactaagtc atatcgtaga taaaaaacct tctgtatcaa
     9121 gaatgtttat taaatccata cctaatatta caaagtataa agaaaatctt attaatcgca
     9181 taaaaatgga attaagtaat gcagatataa gtatttataa taataatatg aaaccattag
     9241 tagaaaagga tataaacgat ac
//
LOCUS       DAMJYR010000135         9254 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.140, whole genome shotgun sequence.
ACCESSION   DAMJYR010000135 DAMJYR010000000
VERSION     DAMJYR010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9254)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9254)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9254
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.140"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            490..1398
                     /gene="trxB"
                     /locus_tag="PC471_003157"
     CDS             490..1398
                     /gene="trxB"
                     /locus_tag="PC471_003157"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HDF2790265.1"
                     /translation="MVDIIVIGAGPAGLTSAIYAMRAGLSVTVFEKNIYGGQVASTSE
                     VENYPAVQKISGVEFSNNIYNQAVAQGVDIQFDEVEEINLEGKVKVVKTSSGEHKAKA
                     VILANGVERRKLGCAGEQEFTGRGVSYCATCDGAFFKDKEVAIVGGGNTALEDALFLA
                     NNCTKVYLIHRRDSFRGEEVLEKSVKARENIEILYSHGVEKIEGEKTVSKIEVKNLKT
                     EEKRTIDVSGIFIAIGLKPNNKMFENVLDLDEGGYIISDESCITSVEGVYVAGDSRTK
                     FLRQIITAASDGAIAAVQAANYINVE"
     gene            complement(1488..3311)
                     /gene="typA"
                     /locus_tag="PC471_003158"
     CDS             complement(1488..3311)
                     /gene="typA"
                     /locus_tag="PC471_003158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HDF2790266.1"
                     /translation="MSQKHKIINIAVIAHVDAGKSTLVDAFLSQSGVFRKNEVVKDCV
                     MDSNDLEKERGITIYSKNCAINYEDYKINIVDTPGHSDFSSEVERVMKTVDTVILLVD
                     ASEGPMPQTRFVLQKSLEFGLKPILFINKIDKKDQRAEEVVNEVFDLFVDLNATDEQC
                     EFPIIYGIAKQGIAKLEMDDDSEDLSPLFKTIVNHVEAYPNYDNEPLQFQISALAYDD
                     YVGRLGIGRIYKGTLKNNTQVAICREDSVVSKGKVSKLSVYEGLKQVEVDEATSGEIV
                     VIAGIPDISIGETICDLDSPLPMEMIKIEEPTLSMNFLVNDSPFVGKSGKFVTTRHLK
                     DRLEKELEVNVGLKVEPLDTTDGYKVSGRGELHLSILLENMRREGYEVGVSKPEVLMH
                     KEDGKLMEPIERVVVNCPEVYSGTIINELNMRKGMMESMSIEGDYVKIEFLAPTRGLL
                     GYRSEFINATRGEGTLVRSFEKFEEFKGEIPSRGNGVLIAQGPGVTMGYSLNALSDRA
                     VMFVDPGVEVYEGMIIGMNSRKDDMVVNPCKNKKMSNVRASGSDDAIKLSPPRIFTLE
                     EALEFIEDDELVEITPDSIRLRKRFLNEHDRLRYNKSRQGK"
     gene            3523..3765
                     /locus_tag="PC471_003159"
     CDS             3523..3765
                     /locus_tag="PC471_003159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2790267.1"
                     /translation="MLEVTKIIIKYFASESDTFVKEEVCNFEELLKWSHKFIEGKKIE
                     DVLYIKSKENYKVNVIKSLEIKELEIVRKQQIPPYM"
     gene            3913..4104
                     /locus_tag="PC471_003160"
     CDS             3913..4104
                     /locus_tag="PC471_003160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1450 domain-containing protein"
                     /protein_id="HDF2790268.1"
                     /translation="MIRVCPNCSNVDVNKLKTLVGEENVKTGCIGQCRAFKKEAVGII
                     DNELEIKETEAEFFEACKE"
     gene            4388..6889
                     /locus_tag="PC471_003161"
     CDS             4388..6889
                     /locus_tag="PC471_003161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HDF2790269.1"
                     /translation="MSSNKVVESYKITGMTCAACAKAVERVTKKMDGVYDQSVNIATE
                     KLKIEYDNSKVNFDDIKQVVEKAGYGIIKEESNKKIDMKIDGMTCAACAKAVERVVKK
                     LDGVESISVNIATDKANIDYDPSKVKLSQIKAAIEKAGYKPIEEVKNKVDVDEDKLRK
                     EREMKSLFVKFIVAIVFAVPLFYIAMGPMIIKPIGPWPLPEIINPMTNTFNYALIQLI
                     LVIPVMIAGYKFYINGFKSLFSLSPNMDSLVAIGTLAAFLYSLYTTLQIANGQIQGMH
                     HHQLYYESAGIIIALILLGKYLESKSKGKTSEAIKKLMGLQPKTAIVLVDGKEVETPI
                     EEVEIGDILLVKPGTKIPVDGVVIEGYTSVDESMLTGESIPVEKNVGSKVTGASINKN
                     GVIKFKAEKIGGDTALAQIIKLVEDAQGTKAPIAKLADTVSGYFVPIVIAIAVVASLL
                     WFLIGGKDIVFVLTIFISVLVIACPCALGLATPTAIMVGTGKGAENGILIKGGEALES
                     AHKVNTVIFDKTGTITEGKPKVTDIVLNNNVKEEYLIKIASSAEKGSEHPLGEAIVKY
                     GEEKNIKFEKVDNFKAIPGAGIQVTINDESILLGNRKLMNDNNIKLGDLEEKSNILAS
                     QGKTPMYIAVDGNLSGIIAVADVVKESSKKAIEILHDMGIKVAMVTGDNAKTANAIAN
                     QVGIDMVLAEVLPEDKSKEVEKLQNQGKFVAMVGDGINDAPALAKADIGIAIGSGTDV
                     AIESADIVLMKSDLIDVPTAIKLSHETIKNIKQNLFWAFGYNTIGIPVAAGILYVFGG
                     PLLNPMIAAAAMSLSSVSVVSNALRLKNFKAYKRD"
     gene            7049..7717
                     /locus_tag="PC471_003162"
     CDS             7049..7717
                     /locus_tag="PC471_003162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2790270.1"
                     /translation="MNSSILVIEDDSNIQELISEFLSAEGYQVDTANDGLEGIQKFKQ
                     GSYDLVILDIMMPNLDGYGVCKMIRKSSSVPIIFLTALNDEGDQLKGFDLECDDYITK
                     PFSFNLLIKRVEAILRRSNKTINDKFIVFEKLKLDLNTYIAEIDGEPIELTLKEFNIL
                     KALIEKYPQVITREGLLDSIWGYDYYGDTRIVDAHIKNIRKKISLPYIKTVKGIGYTL
                     EKDI"
     gene            7701..8978
                     /locus_tag="PC471_003163"
     CDS             7701..8978
                     /locus_tag="PC471_003163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HDF2790271.1"
                     /translation="MRRIFDKWEKLSIKYKLFSITTSLLIALALIIYLILYFLLPSYY
                     HEYKIESLQESLKSLVDSSIHFDTYTLEERLYYMAKDQNLAILLKDNQGKIVYGKNEV
                     VILRYSKYMINSLEDEYRTSIPIYTKDAKDGPYTLELVMPLQPIDEANEVIRKLMPYI
                     ISIAILIAIIGAYIYSIVITKPLINIIESEREQEYRRKDFVATISHELKTPITIISGQ
                     IEGMIYSVGKYKDRDTYLKKSYECTQELKDLVNEMIEVSKSEILEKDLKLVSINISEL
                     LNRLVKRQVFLIEEKHMKTILKIEENLEIKADQERITKAINNIINNAIKYSPEESELI
                     IRLYDKNKRISKKTSNQRVVLEIENTGVTIEKRYLEEIFNPFYRIEKSRSRKTGGSGL
                     GLYIVSQIFKSHGFDYSIKNKENSVVFTVEFKN"
BASE COUNT         3648 a         1077 c         1564 g         2965 t
ORIGIN      
        1 atataagtaa atagtgctaa tatttaaact attaatcata attggttcaa caaaagtata
       61 tagttaataa tgtttaattt gaagaaattg ttagtgtgaa attacatata tattcaaatt
      121 aaattcataa atatttcaca taataatagt atactgtaaa caaaaggagg tattaaatat
      181 gggaatagta cttataacag cagcattagt tttggttatg tatggacttt caattctatc
      241 atatatatca gaaagcaata tattagaaga cactgtaaat gataaccaat aaggttttga
      301 attatataaa taagtgagaa ctgtaaataa aagttaattt ttatttgcag tttttttata
      361 tatatgtaaa ataaagttaa ttaataaaaa ataatgtttt gtttatagta tatatggata
      421 ataatagtaa agtataagaa tgatgagaat aatatataag aaataataat ataaagagag
      481 gtaaacgtta tggtagatat cattgtaatt ggtgctggac cagcaggttt aacttcagca
      541 atttatgcta tgagagcagg gttaagcgtt acagtatttg aaaaaaatat ttatggaggt
      601 caagttgcaa gtacctctga ggtagaaaat tatccagcag ttcaaaagat ttctggggta
      661 gaattttcta ataatatata caatcaagca gttgctcaag gtgttgatat tcagtttgat
      721 gaggttgaag agataaattt agagggaaaa gtaaaggttg taaaaacttc ttcaggtgaa
      781 cataaagcaa aggctgttat tcttgcaaat ggagttgaaa gacgtaaatt aggttgtgca
      841 ggggaacaag aatttacagg tagaggagta tcttactgtg caacatgtga tggagccttt
      901 tttaaagata aagaagttgc tatagtaggt ggaggaaata ctgcactaga agatgcttta
      961 tttttagcta ataattgtac aaaagtgtat ttaattcaca gaagagatag ttttagaggt
     1021 gaagaagtgt tggagaaatc tgtgaaagca agagagaata ttgagatact ttacagtcat
     1081 ggtgtagaaa aaatagaagg agaaaagact gtatcgaaaa tagaagtaaa aaacttaaag
     1141 actgaagaaa aaagaactat agatgttagt ggtattttta ttgctatagg tcttaaacct
     1201 aataataaaa tgtttgaaaa tgttcttgat ttggatgaag gtggatatat aatttcagat
     1261 gagtcttgta taacatcagt agaaggtgta tatgtagcag gagatagtag aactaagttt
     1321 ttacgtcaaa taataacagc agcatcagat ggagctattg cagccgttca agcagctaat
     1381 tatataaatg ttgaataata taaatttcaa ataaaaatag ccatataaac tgaaataatt
     1441 tttcagtact atatggctat tttgttaatt agaggtaaat gtacgtttta tttaccttgt
     1501 ctagatttgt tatatcttaa tctatcatgt tcatttaaga atctttttct aagtcttata
     1561 gaatcaggtg taatttcaac taattcatca tcttctataa actctagtgc ttcttcaaga
     1621 gtaaatattc taggaggaga taattttata gcatcatctg aaccagaagc acgtacgtta
     1681 ctcattttct tgtttttaca agggttgact accatatcat cttttctgga attcatacca
     1741 attatcatac cttcgtaaac ttctacacca gggtctacaa acattacagc tctatcactt
     1801 aaggcattaa gagaataccc catagtaaca ccaggtcctt gtgctattaa aacaccattt
     1861 cctcttgaag gtatttctcc tttaaattct tcaaattttt caaatgaacg aactaaagta
     1921 ccttctccac gagtagcgtt tataaattca cttcgatatc ctaaaagacc acgagtaggt
     1981 gctaagaact ctatttttac atagtctcct tctattgaca tagactccat catacctttt
     2041 ctcatgttta attcatttat aatagtacca gagtaaactt caggacagtt tacaacaact
     2101 ctttctattg gttccattaa ttttccatct tctttatgca ttaaaacttc aggtttagaa
     2161 acaccaactt cataaccttc acgtctcata ttttcaagta gtatagataa gtgaagctct
     2221 ccacgtccag aaactttgta tccatcagta gtatcaagag gttcaacttt aagaccaaca
     2281 tttacttcaa gttccttttc aagtctatct ttcaaatgtc tagttgtaac gaattttcca
     2341 ctttttccaa cgaatggaga gtcgtttact aagaagttca tagaaagagt aggctcttct
     2401 atttttatca tttccatagg tagtggacta tctaaatcgc atatagtttc acctatagat
     2461 atatcaggaa taccagctat aacaactatt tctccactag tagcttcatc aacttcaact
     2521 tgttttaaac cttcatacac agaaagtttt gatacttttc ctttagatac aacagaatct
     2581 tctctacata tagcaacttg tgtattgttt ttaagagtac ctttgtaaat tctacctata
     2641 ccaagtctac ctacataatc atcatatgca agagctgata tttggaactg tagaggttca
     2701 ttatcgtaat taggataagc ttcaacgtga tttacaatag ttttaaatag aggagaaagg
     2761 tcttcactat catcatccat ttcaagttta gcaatacctt gtttagcaat accatatatt
     2821 ataggaaatt cgcattgctc atctgttgca tttaagtcta caaataaatc gaaaacttca
     2881 ttaactactt cttccgctct ctggtctttt ttatcaatct tgtttataaa taatattggt
     2941 tttaaaccga actctaaaga tttttgtaaa acgaatctag tttgaggcat tgggccttca
     3001 ctagcatcaa ctaatagaat aactgtgtca acagttttca taacacgttc aacttcagaa
     3061 gaaaagtcac tgtgtcctgg agtatcaact atgtttattt tgtaatcttc atagtttatt
     3121 gcacagtttt ttgaatatat agttatgcct ctttcttttt caaggtcgtt actgtccata
     3181 acgcagtctt taactacttc gttctttcta aaaacaccgc tttgagaaag gaaagcatca
     3241 accaatgttg attttccagc gtcaacgtgg gctataactg cgatatttat aattttatgt
     3301 ttttgtgaca tttttgttgc tcccttcgat taaaataact tattaattat agcacaatat
     3361 attattaggt acaaggagga atatattaaa ttgtattaat atagtttaaa ttgaataaaa
     3421 ctgttagttt tgcatctgaa aattattttg tttaaaaaaa ttttttagac aaataataac
     3481 cttaatagat aaaaagatta ataaaagagt aaaggatgat atatgttgga agtaaccaaa
     3541 ataataatta aatattttgc aagtgaaagt gatacttttg taaaagaaga agtttgtaat
     3601 tttgaagaac ttcttaagtg gtctcataaa tttattgaag gtaaaaagat agaagatgtt
     3661 ttgtatataa agtctaaaga aaactataaa gtaaatgtga ttaagtcttt agaaattaag
     3721 gaattggaaa tagtgagaaa acaacagata ccaccgtaca tgtagtatta ataatatata
     3781 aaattaatat gtaatactcg caataggata taaatggtat atatggtaat aatttatatt
     3841 aaaaaaactt ttataaagtt tatatttggg tataaaagta ttaattagac attaattagg
     3901 aggaacttaa atatgataag agtatgtcca aactgttcaa atgtggatgt aaataagcta
     3961 aaaactttgg ttggagaaga aaatgtaaag actggttgta taggtcagtg tagagcattt
     4021 aaaaaagaag ctgttggaat aatagacaat gagcttgaaa taaaggaaac agaagcagag
     4081 ttttttgaag catgtaaaga ataaatttta aataaaatat aataaataga gcatctaaat
     4141 tgtataagca ttaaacaatt tagatgctct atttttatat atacatttaa ttatgagtat
     4201 acttttctat taaatacaaa taaatataac taatacattt atttattaaa aaaatattga
     4261 tttaatatac ctataggggg tataataaaa atatagattt ctaaataagt aatttatcaa
     4321 tattacatat caaaataaaa cgttacaaat tagctgaaaa tatattgaaa gaaaggaaga
     4381 ttttattatg agttcaaata aagtggtaga aagttataaa attacaggta tgacatgtgc
     4441 agcttgtgca aaggcagtag agagagtaac taagaagatg gatggagtat atgaccaaag
     4501 tgtaaatata gctactgaaa aacttaaaat agaatatgat aattctaagg ttaattttga
     4561 tgatataaag caggttgtag aaaaagcagg atatggaatt ataaaggaag aaagtaataa
     4621 aaaaattgat atgaaaattg atggaatgac atgtgcagct tgtgcaaaag ctgtagagag
     4681 agtggtaaaa aaattggatg gagtagaaag tattagtgtg aatattgcaa cagataaagc
     4741 taatatagac tatgacccat caaaagtaaa attatctcaa ataaaagcag ctattgaaaa
     4801 agctggatat aaaccaatag aagaggtaaa aaataaggtt gatgtggatg aagataaatt
     4861 gagaaaagaa agagaaatga agtctttatt tgttaaattt atagttgcaa tagtatttgc
     4921 agtacctctg ttttatatag caatgggacc tatgataata aagcctatag ggccatggcc
     4981 tttgccagaa attataaatc ctatgacaaa tacttttaat tatgcattaa ttcagttaat
     5041 tttagtaata ccagtaatga tagctggtta taaattttat ataaatgggt ttaaatcact
     5101 tttttcttta agccctaata tggattcttt agttgcaata ggaacattag ccgcattttt
     5161 atatagtcta tatacgacac tacaaatagc caatggacaa attcaaggta tgcaccatca
     5221 tcagttgtat tatgaaagtg caggaataat aatagcactt atcttacttg gtaaatattt
     5281 agaatcaaaa tcaaaaggaa aaacatcaga agcaattaaa aagcttatgg ggctacaacc
     5341 taaaactgca atagtgttag ttgatggtaa agaagttgaa actcctatag aagaagtaga
     5401 gataggagat atactattag taaaaccagg aactaaaata cctgtggatg gagttgtaat
     5461 agaaggatat acatcagttg atgaatcaat gcttacaggt gaaagtatac cagtagaaaa
     5521 gaatgttgga agtaaggtga caggagctag tattaataaa aatggtgtta ttaagtttaa
     5581 ggctgaaaaa attggtggag atactgctct tgcccaaata ataaagttag ttgaagatgc
     5641 acaaggtaca aaagcaccta ttgctaaact tgccgatact gtatctggat attttgtacc
     5701 aatagtaatt gcaatagctg tagttgcatc attattgtgg tttttaatag gaggaaaaga
     5761 tatagtattt gtacttacta tatttatatc agtacttgta attgcttgtc cttgtgcatt
     5821 aggattagca acaccaactg ctataatggt tgggactgga aagggcgcag aaaatggaat
     5881 cttgataaaa ggtggagagg cattagaatc tgcacataaa gtaaatacag tgatatttga
     5941 taaaacaggt actataacag aaggaaagcc aaaagtaaca gatatagtat taaataataa
     6001 tgtaaaagaa gaatatctta tcaagattgc ttcaagtgca gaaaaaggtt cagaacatcc
     6061 tcttggagaa gcaatagtta agtatggaga agagaagaat ataaaatttg aaaaagttga
     6121 taattttaaa gccataccag gtgctggtat acaagttact attaatgatg aaagtatatt
     6181 acttggaaat agaaagctta tgaatgacaa taatataaag cttggggatt tagaagaaaa
     6241 atcaaatata ttggcaagcc aaggtaaaac acctatgtat atagctgtag atggaaattt
     6301 atcaggtata atagcagttg cagatgtagt caaagaaagt agtaaaaaag caatagaaat
     6361 acttcatgat atgggaataa aagttgcaat ggtaactgga gataatgcga aaactgcaaa
     6421 tgctatagca aatcaagtcg gtatagatat ggttttggca gaggttttac cagaagataa
     6481 gtcaaaagaa gttgaaaaac tacaaaatca aggaaagttt gttgcaatgg taggggatgg
     6541 aataaatgat gccccagctc ttgcaaaagc agatataggg atagcaatag gaagtggaac
     6601 ggatgtagcc attgaatctg cggatatagt acttatgaag agtgatttaa ttgatgttcc
     6661 aacagcaatt aaacttagtc atgaaactat aaaaaatata aaacaaaacc tattttgggc
     6721 atttggatat aatacaattg gtatacctgt agcagcagga atattatatg tatttggagg
     6781 acctttatta aacccaatga ttgcagcagc agcaatgagc ttaagttcag tttcagtcgt
     6841 atcaaatgca cttagactta aaaacttcaa ggcatataag agagattaga ttaataaatt
     6901 aaataaaatg tatccaaaag agctatcttt aaattaaaag gattaattta caaattaaga
     6961 tagctctttt ttcatgtaaa attcacaaca ataagataaa atataagtaa tatacgaaat
     7021 tttaaataaa atagaaagga ttgatagaat gaattcgtca atacttgtaa ttgaagatga
     7081 ttcaaatata caagagttaa tatcagaatt tttaagtgca gaaggttatc aagttgatac
     7141 agcaaatgat gggttggaag gtatacaaaa gtttaagcaa ggaagttatg atttagtaat
     7201 attagatata atgatgccaa acttagatgg ttatggagtt tgtaaaatga taagaaagtc
     7261 atcaagcgtt ccaattatat ttttaactgc tttaaatgat gaaggagacc aacttaaagg
     7321 gtttgactta gaatgtgatg attatataac aaaaccattt tcatttaatc ttcttataaa
     7381 aagagtagaa gcaattttaa ggcgaagtaa caagactata aatgacaaat ttatagtttt
     7441 tgaaaaatta aaattagatt taaacacata tattgcagag atagatggag aacctataga
     7501 acttacacta aaagagttta atatattaaa agctttgata gaaaagtatc cacaagttat
     7561 aactagggaa ggtcttttgg atagtatatg gggttatgac tattatggag atactaggat
     7621 tgttgatgca catataaaaa atataagaaa aaaaatatca ttaccatata ttaaaactgt
     7681 aaaaggaata ggatatacac ttgagaagga tatttgataa gtgggaaaag ttaagtataa
     7741 agtataagtt attttcaata acgacatcat tattaatagc attagcttta ataatatatc
     7801 taattttata ttttttattg ccatcatatt atcatgagta taaaattgag tctcttcaag
     7861 aaagtttgaa atctttagta gatagttcaa ttcatttcga tacatatact ttagaagaaa
     7921 gactttatta tatggctaaa gaccaaaatt tggctatttt attaaaagat aaccaaggta
     7981 aaattgtata tggtaagaat gaagtagtta ttttaagata tagtaagtac atgattaata
     8041 gtcttgagga tgagtataga acttctatac ctatatatac taaagatgca aaagatggtc
     8101 catatacttt agaactcgtt atgccattac aaccaattga cgaagcaaac gaagttataa
     8161 gaaaactaat gccatacatc atatcaatag caatattgat agcgattatt ggagcatata
     8221 tatattcaat tgtaataaca aagcctctta taaacataat agagagtgag agagaacagg
     8281 aatatagaag gaaggatttt gttgctacaa tatcacatga actaaaaact ccaataacca
     8341 taataagtgg tcaaattgaa ggtatgattt atagtgtagg taagtataag gatagggata
     8401 cttatctgaa aaaatcgtat gagtgtacac aagaattaaa agatttagta aatgaaatga
     8461 tagaggtttc aaaatcagaa atacttgaaa aagatttgaa attggtatca ataaatataa
     8521 gtgaattatt aaatagactt gtaaagagac aagtattttt aatagaagaa aaacatatga
     8581 aaactatttt aaaaatagaa gaaaatttgg aaattaaagc agaccaagag agaataacta
     8641 aggcaatcaa taatataata aataatgcca taaaatattc tcctgaagaa tcagagctta
     8701 taattagact ctatgataaa aataaacgaa taagtaaaaa gacttctaat caaagagtgg
     8761 ttttagaaat tgaaaatact ggtgtaacta tcgagaaacg ttatttagaa gaaatattta
     8821 atccatttta tagaatagaa aaatctcgtt cgagaaaaac tggaggaagt ggacttggac
     8881 tttatatagt cagccaaatt tttaaaagtc atgggtttga ttatagcata aaaaacaaag
     8941 aaaactctgt agtattcaca gtagaattta aaaattaatt acttattata aagaatatat
     9001 aactttcaat aatagttcat tttaaaatca caaaaaattc ataatgaaac tgtattatat
     9061 aaatataaat aagcttaaaa ccataaaata caccaactat aatgatgaga tttccttaca
     9121 tcctccatat aagtcatcat tatagtaacc caacaattaa gttactatga atttaaaaaa
     9181 taacaccgag catataatct atgcttagtg ttatttttat tttgaaataa aaaatattta
     9241 atttattgaa atgt
//
LOCUS       DAMJYR010000136         9217 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.141, whole genome shotgun sequence.
ACCESSION   DAMJYR010000136 DAMJYR010000000
VERSION     DAMJYR010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9217)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9217)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9217
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.141"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            133..1332
                     /locus_tag="PC471_003164"
     CDS             133..1332
                     /locus_tag="PC471_003164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2790272.1"
                     /translation="MKLKSKISIFIIFLTLAIYFSIGLLNNEDNLKIKTIKGDSNELD
                     HININFGYALNGYINSYYTINGSNKTKKTELIKLQKDSKNTNNLIDVSSGSDLIAHVG
                     FEMNEESKDINFVIDEKKEDLEKGVFPSEGSDKEIEYEKLDMNNFTRTRYKVDKSTDD
                     YYAKILASKRYKKYLYVSVLYATSDSSENNKEYSSTIDIIKINPENKKIKTIKSINLS
                     NYFSEKYQNIENIGVSYASKYEDKMYILVNVLNTNIGDNSNSKFHLLEYNLEENKESI
                     MELKTDSKMALAKAKLEANKLYMVMSNDYDNSSITELNLLTYNILDKSLDEDKYVLDN
                     REKRGIRSIEIDSNKIVAYTSRLNSILSWDDKFMLYIINKNSKEIVYEGELQFNSSNS
                     MLEFEIK"
     gene            1341..2153
                     /locus_tag="PC471_003165"
     CDS             1341..2153
                     /locus_tag="PC471_003165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2790273.1"
                     /translation="MKDLIRLFEFELKRNIKNYIFIIICCCSFVILNIVKNLNDYNYI
                     IENAVKSEQLTKIGNTIETVNVTGFSSFRNIMGSTESWFMFGIIACICYAFFIWYRDF
                     NGRSKSIYTLIMLPKNRINIYISKLLNILFLVYSYTVVLTISLFIASKLLPRHMLGNV
                     TNYGFVQETIYELKMLLPYSFEILFVEYIFLLIGFVSVVFTSILINKSIYKVSTLISF
                     LFLVIEILVFIISIEFIIPYEYSDVFTVLYSSINIFVCSLISNKLLKQKIDF"
     gene            2164..3432
                     /locus_tag="PC471_003166"
     CDS             2164..3432
                     /locus_tag="PC471_003166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2790274.1"
                     /translation="MKAKNIGKYTICFIIVCFLMITMTLILSYFNKDKLSVKTLKGNV
                     KDIGRVSVVYTPNISKFSYEEVIISKNGVRNNTNPKSRIRNDYLNIDKKYSDFVNDKG
                     KEFIYKDDLNIGEAYTSVKSDLVQGMNALNDCIKVSNKNLKSGVEENFDINLNKSDFK
                     ENSWKTLFNKIYKGNLYMVISETNISGSKVNNICILKIDIKNKSYKLLNRFNLGLQNG
                     EQQNINEIKFSIGSKLYLEIVNNKSKNGYIEGHDSYSNFIIYDIEDNTFNISNKFMDN
                     LNKEKNANPKEIGLLSKTKLDYMVKNNKLNLIVTDMSNSITNLVYDINSNEIKLEDYE
                     ILDLKVDYNEASSGVRKVKLLDDKIYSISGIKNSISGELKGFKGIIDVVGTKPVKFQV
                     FDMKLKKNIYEAELINGDISLQEKLFFVAN"
     gene            3798..4979
                     /locus_tag="PC471_003167"
     CDS             3798..4979
                     /locus_tag="PC471_003167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HDF2790275.1"
                     /translation="MNKKYIKAGNLIDGTGNSIMQNKGIIIDGDTIVEIEDIRDDLNG
                     YDVYDYLDKTVMPGIMNCHVHLTMEPVGNPKIYYDNVSDVELVVNTIKQLDAYLDSGV
                     TYIRSLGCPKYVDVQLKNLIDKGSIDGPGIVTSGPVICMTGGHGYYFGIESDGVDECR
                     KSARTVLKNGVDCVKIMATGGVTTDGVEPGSPQLTLEEMKAAVDEAIKAGKITATHAQ
                     GRTGIKNAVLAGITSIEHGVYLDDEIIDLMLERGTYLVPTVAAPYFILNAGKESGITE
                     ATLRKCEIVAIPHKESFLKAYKKGIKIAVGSDAGTSYNEHGKTYYEMKLMSDYGMDNM
                     DIIVAATKTASELLRIDKNYGTLEKGKKADVIVVNGNPVENIDVLADVLAVFKLGKKV
                     R"
     gene            complement(5121..6038)
                     /locus_tag="PC471_003168"
     CDS             complement(5121..6038)
                     /locus_tag="PC471_003168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2790276.1"
                     /translation="MENNKCREDFRFTQEYEEDYPNTNERYYENYQVADRYYNYPNKY
                     KEPKIKQCCCKKSMREALELLRYDALRPFVNFNQFAFISDFFIVGANLVGIDLSAPPK
                     DNLSGLDGTFERFSACNCDLIDIAGRVSYPIPVPLTLEGLINTIGTIPGVAELIALID
                     AVIPPTIDLGAILDAILAAIIDFILAASTPLANVDLASLCNLKAVAFDITPADYEDFI
                     ASLGYYLDKKHYKECNCNCDCDDCCCNKGILDNLYMSNINNQVTVVAGSLVLTGVEVL
                     GKKNDVIVLGNSNDSRIYFVCVDSIDYIA"
     gene            6291..7085
                     /locus_tag="PC471_003169"
     CDS             6291..7085
                     /locus_tag="PC471_003169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2790277.1"
                     /translation="MIEVDKLCKSFTRVVKDDKNKSSIRKLKKVKTKKEEFFAVNNVS
                     FKVMEGEIVGILGPNGAGKTTLLRMLGGILTPTSGSINISGYDYSIDRNSAKKEIGYL
                     SGNTKLYGRLSPRELLTTFASLYEMSKEDIEESIENIVKIMDMSEFIDNRIENLSTGQ
                     TQRTSIARCLIHSPKVYIFDEPTLGLDVLSSASIIDFMKNEKLRGKTVLYSTHYMEEA
                     ETLCDKIFMIHNGKIIASGTPQSLKEESGVNNLRDVFIKLARKEDI"
     gene            7086..9146
                     /locus_tag="PC471_003170"
     CDS             7086..9146
                     /locus_tag="PC471_003170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HDF2790278.1"
                     /translation="MRSKIVKYIFKKEMLDILRDKKTLFMMIVVPLLLYPLLMLLMIQ
                     VMNMSASSMNSKDINIAFNNKPNKVLVSMIEDDNLEKDTKNTSNDKGKIRVLDVDNYK
                     KSLEENKIDAYIKMEEKKSSINYQIYINSSQENSSNALKRIETVLDKYKRFTIEKTLS
                     EKGLDVQATLEPITYSTVDVAKTEEVAGYFLGQILPFILIIGVLLGSIYPAIDVMAGE
                     KERGTLETLFTLPISNLELVMGKYMAVSLSAIVTAILNILSMGLTMAFILVSGGISSQ
                     FGFGNFNYGDLVLPIITTLICICLFSMVVSAISMCVCSLAKSFKDAQNYITPLMLIIM
                     IPSYVSMIPNIVLDRVTAVIPVVNISLLIKSVLSFKSDLNLIALVLASNVAFVILAII
                     LLSKMFNSEEILFGNSKSFSFLEKRSNIKKGSIPTVSDGVILYAVGLVLLIYVGSLVQ
                     IKFGMAGIIMTQFMIISLPLLFAYYIKADFKEVFSLTVPKLKHVFGSIVLWIGGYILI
                     MIITQLILFLFPQNMEVAESLNKALFIKDSVLLNLLAIALLPAICEEMFFRGFIFSSF
                     SKSKDKNKSIKLAIICSGVLFGIMHMDFIRIIPTSILGIIFAYSVYKSGSIFVSMLLH
                     FLNNSVAVVLNHYTTGNITNLYKFVEVDFSNLNVLKFALILLISLILIMLGLRVLDRK
                     SLRN"
BASE COUNT         3686 a          909 c         1459 g         3163 t
ORIGIN      
        1 ttataatctt ataggatttc aatttaacaa tttagtagca gggttattaa gttttttaat
       61 tataatgttt attctaaatg gaatatctta tttgctaatt aaaaagaaag tatctcttta
      121 aggaggggtg gtatgaaatt aaaatctaaa atatctatat ttattatatt tttgacttta
      181 gcaatatatt ttagtatagg tttattaaat aatgaagata atttaaagat aaaaactata
      241 aaaggtgact ctaatgaatt agaccatata aacataaatt ttggatatgc tttgaatgga
      301 tatataaatt catattatac aataaatgga agcaacaaaa ccaaaaagac tgaattaata
      361 aaactacaaa aggatagtaa gaacacaaat aatttaattg atgtatctag tggttcagat
      421 ttaattgctc atgttggatt tgaaatgaat gaggagtcaa aagatataaa ctttgtgata
      481 gatgagaaaa aagaagattt agaaaaaggt gtatttccat cagaaggtag tgataaagaa
      541 attgaatatg aaaaattaga tatgaataat tttacaagga caagatataa agtagataaa
      601 agtacagatg attattatgc aaagatacta gcatcaaaaa gatataaaaa atacttatat
      661 gtatctgtac tttatgctac aagtgattca tcagaaaata acaaagaata ttcaagtact
      721 atagatataa taaaaattaa tccagaaaat aaaaaaatca agactataaa aagtataaat
      781 ttaagtaatt atttttccga aaaataccaa aacatagaga acataggtgt aagttatgct
      841 agcaaatatg aggataagat gtatatttta gtaaatgtac taaatacaaa cataggtgat
      901 aatagtaatt ctaaatttca tttacttgag tataatcttg aagaaaataa ggaaagcata
      961 atggaattaa aaactgatag caaaatggct ttagcaaaag caaagttaga agctaataaa
     1021 ttatatatgg taatgtcgaa tgattatgat aatagttcaa ttacagagtt aaatttactt
     1081 acctataata tattagataa aagcttagat gaagataaat atgttttaga taatagagaa
     1141 aaaagaggaa ttagaagtat tgaaattgat tcaaataaaa tcgttgcata tacatcaagg
     1201 ctaaactcta tattatcttg ggatgataaa tttatgctat atataataaa taaaaattct
     1261 aaggaaattg tatatgaagg agaattacaa tttaacagtt caaatagtat gttagaattt
     1321 gaaataaagt aggtgagttg atgaaagatt taataagatt attcgaattt gaattaaaaa
     1381 gaaatataaa gaattatata tttataataa tttgttgttg ttcttttgta attttaaaca
     1441 tagtaaaaaa tttaaatgac tataattaca taatagaaaa tgcagttaaa agtgaacagt
     1501 taacaaaaat cggaaatacg atagaaacgg taaatgttac aggtttctca agctttagaa
     1561 atataatggg ttctacagaa tcgtggttta tgtttggtat aattgcatgt atttgctatg
     1621 ctttttttat ttggtataga gactttaatg gtagaagtaa atctatatat acacttataa
     1681 tgctacctaa aaatagaatc aatatttata tatctaaatt attaaatata ttatttctag
     1741 tgtatagcta tacagtagta cttacaatat ctttgtttat tgcaagtaag ttgttgccta
     1801 gacatatgct tggtaatgtc acaaactatg gttttgtaca agaaacaata tatgagttaa
     1861 aaatgttact tccatatagc tttgaaatac tatttgtaga gtatatattt ttattgatag
     1921 gatttgtatc tgtagtattt acatcaattc ttataaataa atctatttat aaagtaagca
     1981 ctttaataag ttttctattt ttagttatag aaattttagt gtttataatt tctatagaat
     2041 ttattatacc ttacgaatac agtgatgtat ttactgttct atattcaagt ataaacatat
     2101 ttgtatgtag tcttatatct aataaacttt tgaagcaaaa gattgacttt taggagggat
     2161 attatgaaag caaaaaatat aggaaaatat acaatatgtt ttataatagt atgtttttta
     2221 atgattacta tgaccttaat attgagttat tttaataagg ataaactatc tgtaaaaact
     2281 ttaaaaggca atgtaaaaga tatagggaga gtaagtgtag tatatacacc taatataagc
     2341 aaattttctt atgaggaggt aattataagt aaaaatggag taagaaataa tacaaaccca
     2401 aaatcaagaa ttagaaatga ttatttaaat attgacaaaa aatacagtga ctttgttaat
     2461 gataaaggaa aagaatttat atataaagat gatttgaata taggagaagc gtatacctct
     2521 gttaagtctg atttagtaca aggaatgaat gctttaaatg attgtataaa ggtttcaaat
     2581 aaaaatttaa aaagtggggt agaagaaaat tttgatatta atttaaataa gtctgatttt
     2641 aaagaaaata gttggaaaac cttatttaac aaaatatata aaggtaattt atatatggta
     2701 attagtgaaa ctaatatttc aggttctaaa gtcaataata tttgtatatt aaagatagat
     2761 ataaaaaata agtcttataa attattaaat agatttaatt taggattaca gaatggagaa
     2821 caacaaaata tcaatgaaat taaattttct attggaagta aattatactt agaaatagta
     2881 aataataaaa gtaaaaatgg atatattgaa ggacatgata gttactctaa ctttattata
     2941 tatgatatag aagataacac ttttaatata agtaataaat ttatggataa tctaaacaaa
     3001 gaaaagaatg caaatccaaa ggagattggc ttattaagta aaactaagct agattatatg
     3061 gtaaaaaaca ataaattgaa tcttatagtt acggatatga gtaattccat aactaatctt
     3121 gtttatgata ttaattctaa tgagattaaa ttagaagatt atgagatact tgatttaaaa
     3181 gtagattata atgaagcatc atctggagtt aggaaagtta agctactgga tgataagata
     3241 tattctattt caggtattaa gaacagtata tcaggagaat taaaaggatt taaagggatt
     3301 attgatgtag tcggaactaa gccagtaaaa tttcaagtat ttgatatgaa acttaaaaag
     3361 aatatttatg aagctgagct aataaatgga gatataagtt tacaagaaaa gttatttttt
     3421 gttgcaaatt aagtgttaga attaatttta gaactttaaa cttttatatg tatcacagat
     3481 aagtataagt aagggagtgt ctcaaaataa actttttagc tcatgagaca ctttttttat
     3541 atgaaatcaa atatattttc attgtttaga tttattttga taatgaaaaa aagattatct
     3601 ttattttaag aaagacattt atttataatt atatacaaaa atatttttta atgtttttgt
     3661 gtataattat aacaatttaa tttatattaa tgcacttaaa tttgaaatag aatgcttata
     3721 gtggtaaaat taattattag aatattagaa ataataaata aataatacat tgtacttaaa
     3781 agggggagaa atttaagatg aacaaaaaat atattaaagc agggaattta attgatggaa
     3841 ccggaaatag tattatgcaa aataagggta taattattga tggagatact atagttgaaa
     3901 ttgaagatat aagagatgat ttaaatggat atgatgtgta cgattactta gataaaactg
     3961 ttatgccagg cattatgaat tgtcatgttc acttgacaat ggagccagtt ggaaatccaa
     4021 agatatatta tgataatgta tcagatgtag agcttgtagt aaatacgata aaacaacttg
     4081 atgcttattt ggattcagga gtaacatata ttagaagctt aggttgtccc aaatatgtgg
     4141 atgtacaact taaaaatctt atagacaaag ggtctataga tggtcctggt atagttactt
     4201 caggtccagt tatatgtatg acaggagggc atggatatta ttttggtatt gaaagtgatg
     4261 gagtagatga atgtagaaaa tcagccagaa cagttttaaa aaatggagta gattgtgtaa
     4321 agataatggc aactggaggt gtaacaactg atggagttga gccagggtca ccacaattaa
     4381 ctttggagga aatgaaggct gctgtagatg aagctataaa agcaggtaaa ataactgcca
     4441 cacatgcaca aggaagaact ggtataaaaa atgcagtgct tgcaggaatt acttctatag
     4501 aacatggtgt ttatttagat gatgaaataa ttgatttgat gcttgaaaga ggaacttact
     4561 tagttccaac tgtggctgca ccatatttta ttctaaatgc aggaaaagaa agtggaataa
     4621 ctgaagctac tcttagaaag tgtgagattg ttgccattcc acacaaagag tcattcctaa
     4681 aagcatataa aaaaggaatt aaaattgcag ttggttctga tgcaggaact tcttataatg
     4741 aacatggaaa aacatattat gaaatgaaac taatgtcaga ttatggtatg gataatatgg
     4801 atataatagt tgctgctaca aaaacagcat ctgaactttt aagaattgat aaaaattatg
     4861 gaactctaga aaaaggaaag aaagcagatg ttatagttgt aaatggaaat ccagtagaga
     4921 atatagacgt acttgcagat gtgttagcag tatttaaact tggtaaaaaa gttagataaa
     4981 tagtataaaa ataaaaaaga gtgtctaaag aaatttttaa ataattatag acactctttt
     5041 ttattagtaa taattaaatc ttttatactt tagaaaatag attctatttt atatttagat
     5101 ataactcttt aaaatcattc ttatgcaata taatctatag aatctacaca tacaaagtat
     5161 attcttgaat cattagaatt tccaagtact ataacatcat ttttcttacc tagaacttca
     5221 acaccagtta gaaccaaact accagctact acagtaactt gattatttat atttgacata
     5281 taaagattat ctaggatacc tttattacaa cagcaatcat cacaatcgca gttgcaatta
     5341 cattctttgt aatgtttttt atcaagatag taacctaaag atgctatgaa atcttcataa
     5401 tctgcaggtg taatatcaaa tgcaacagct ttaagattac acaatgatgc taaatctacg
     5461 tttgctaatg gagtagatgc agcaagtata aaatcaatta tagcagcaag tattgcatct
     5521 aatatagccc caaggtctat cgtaggagga ataactgcat caataagtgc aattaattca
     5581 gctactcctg gtatagttcc tatagtatta attaatccct caagagttaa agggactgga
     5641 ataggataag atactctacc agctatatct attaaatcac agttacaagc agaaaatctt
     5701 tcaaaagtac catcaagtcc agataaatta tcttttggag gagctgaaag atctatacct
     5761 accaaattag cacctactat aaagaaatct gagataaaag caaattgatt aaagtttaca
     5821 aaaggtctta gagcatcata tcttagaagt tctaatgctt ctctcatact ttttttacaa
     5881 caacattgtt ttattttagg ttctttatat ttatttggat aattatagta tctatcagct
     5941 acttgataat tttcatagta tctttcattt gtatttggat aatcttcctc atattcttgt
     6001 gtaaatctaa agtcctctct acatttatta ttttccacta aatacactcc ttttatattt
     6061 taatttaaat gtatagataa ttaagcttat ctatatagta atattatatt tataatgtaa
     6121 aaaaatgtta caaaagatat tataaacgaa taaaaaataa taatttgtca gataaacttg
     6181 taaattttta tgaataatcc attctaaaac aaaaaactgt gtataaaaaa ggtttatatg
     6241 ttatatttat ataaatataa catatgaacg agaaaggagt ggtttgataa atgattgagg
     6301 tagataaatt gtgtaagtct tttacaaggg ttgtaaaaga tgataagaat aaaagttcaa
     6361 taagaaagct aaagaaagta aaaactaaaa aagaagaatt ttttgctgta aataatgttt
     6421 catttaaagt tatggaaggt gaaattgttg gaatattagg tccaaatggt gcaggaaaga
     6481 ctacattact tagaatgtta ggtggcatat taacaccaac atcaggaagt ataaatattt
     6541 ctggatatga ttattcaata gatagaaatt cagcaaaaaa agaaataggt tatctctctg
     6601 gaaatacaaa actatatgga aggctttctc cgagagaact tctaacaact tttgcaagtc
     6661 tatatgagat gtcaaaagaa gatatagaag aatcaattga aaatattgta aaaataatgg
     6721 atatgagtga atttatagat aatagaattg aaaatctatc aactggtcag actcaaagaa
     6781 catctatagc tcgttgttta atacattctc caaaagtata tatttttgat gagccaacac
     6841 taggtctgga tgttttaagt agtgcatcta ttatagattt tatgaagaat gaaaaactaa
     6901 ggggaaaaac tgttctttac tcaactcatt atatggaaga agcagaaact ttatgtgaca
     6961 agatttttat gattcacaat gggaaaataa ttgcatcagg aacaccacag agtttaaaag
     7021 aggaatctgg agtaaataat ttgagagatg tattcataaa acttgccaga aaggaggaca
     7081 tttaaatgag aagtaagatt gtaaaataca tatttaaaaa agaaatgtta gacatattga
     7141 gagataaaaa gactttattt atgatgattg ttgtgccact tcttttatat cctttactta
     7201 tgttattaat gattcaagta atgaatatgt ctgcaagtag tatgaatagt aaggacatta
     7261 atatagcttt taataataaa cctaataagg tgttagtatc catgattgaa gatgataatt
     7321 tagaaaaaga tactaaaaat acttcaaatg ataaaggcaa aataagagtg ttagatgtag
     7381 ataactataa aaagtcatta gaagaaaata aaattgatgc ttatataaag atggaagaaa
     7441 aaaaatcatc tataaattat caaatatata taaattcttc tcaagaaaat tcttctaatg
     7501 cactaaaaag aattgaaact gttttggata aatataaaag atttaccata gaaaaaactt
     7561 tatctgaaaa aggattagat gtacaagcta ctttagaacc aataacatat agcactgtag
     7621 atgttgcaaa gacggaagaa gtggcaggat actttttagg tcagatactt ccatttatat
     7681 taataatagg agttttacta ggttcaatat atccagcaat agatgttatg gctggagaaa
     7741 aggaaagagg tacattagaa acattattta cactgccaat atcaaactta gaactggtta
     7801 tgggaaaata tatggcagtt tctcttagtg caatagtaac agcaatttta aacattttat
     7861 ctatgggact gactatggca tttattttag ttagtggagg tattagtagc caatttggct
     7921 ttggaaactt taattatgga gacttagtct taccaattat aactacttta atttgcatat
     7981 gtcttttttc aatggtagtt tcagcaataa gtatgtgtgt atgttcatta gcaaaaagtt
     8041 ttaaagatgc tcaaaactac ataacacctc ttatgttaat tattatgata ccatcatatg
     8101 tttctatgat accaaatata gtattagata gagtgacagc tgtaatacct gtagtaaata
     8161 tatctttact tataaaaagt gtattatctt ttaaaagcga tttaaattta atagccttag
     8221 ttttagcatc aaatgtagcc tttgttatat tggcaatcat actactgtct aagatgttca
     8281 attctgagga aatacttttt ggtaatagta agagtttttc tttcttagag aaaagaagta
     8341 atataaaaaa agggtctata ccaactgtaa gtgatggtgt tattttatat gcagtaggat
     8401 tagttctatt gatttatgtt ggttctttag ttcaaattaa atttggtatg gcaggtatta
     8461 taatgacaca attcatgata atttctttac cattattatt tgcatactat attaaagctg
     8521 attttaaaga ggtatttagc ttgacagttc ctaaattaaa gcatgtgttt gggagcattg
     8581 ttctttggat tggtggatac atacttatta tgattataac acagctaatt ttattcttat
     8641 ttcctcaaaa tatggaagtt gctgaaagtt taaataaggc tctttttatc aaagatagtg
     8701 ttttactgaa tttactagca atagctttat taccagctat atgtgaagaa atgtttttta
     8761 ggggatttat attttcttca tttagtaaat caaaagataa aaataagtct atcaaactcg
     8821 ctataatatg tagtggggta ctatttggta ttatgcatat ggattttatt agaataattc
     8881 caacatcaat attaggtatt atatttgcat acagtgtgta taaatctggt tcaatatttg
     8941 tatcaatgtt attgcatttt ttaaataata gtgtagcagt tgtgttaaat cattatacaa
     9001 ctggaaatat aaccaactta tataaatttg ttgaagttga tttttcgaac ttaaatgtgc
     9061 ttaaatttgc cttaattctt ctaataagtc ttatattaat tatgttagga ttaagagttt
     9121 tagatagaaa gtctttaaga aattaagtat aaagttatta tatagattaa aatattaata
     9181 agcactaaag tatatggata tatcttgtat ataaaaa
//
LOCUS       DAMJYR010000137         9215 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.142, whole genome shotgun sequence.
ACCESSION   DAMJYR010000137 DAMJYR010000000
VERSION     DAMJYR010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9215
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.142"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(<1..206)
                     /locus_tag="PC471_003171"
     CDS             complement(<1..206)
                     /locus_tag="PC471_003171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HDF2790279.1"
                     /translation="MIIHKFIIHVLDKNSDVPILNDFEGKVNQEVDGFFQKAIKRIAK
                     DEDLRKGVFKDYNDNLIKNCCEQI"
     gene            complement(220..1122)
                     /locus_tag="PC471_003172"
     CDS             complement(220..1122)
                     /locus_tag="PC471_003172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HDF2790280.1"
                     /translation="MFKKENQRYNLISYTNEEEMTLKEVLLDKLNFSVRSLSKMKREK
                     SVLVNGVYKKPSLKVYSGDLIEVKIDEEKANFEPQDLNLQIIYDDFDIIMVNKPPFMV
                     VHPTKSHYDKTIANGISYYIDNQKENVKIRFVNRLDMNTSGLVIVAKNAYAHHTLSTA
                     MSENKVEKKYITVVDGIIKENEGTIDEPIYRPTEDSIKRIIDERGQSSVTHYKVIERL
                     ENATVLEVSLETGRTHQIRVHMAHIGHGIIGDELYGYVDEELINRQALHAYKLEFEQP
                     RTKEKLKFKADIPEDMKELISKLR"
     gene            complement(1317..2492)
                     /locus_tag="PC471_003173"
     CDS             complement(1317..2492)
                     /locus_tag="PC471_003173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000786547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HDF2790281.1"
                     /translation="MREVVIASAARTAVGSFGGAFKSVSAVELGVTAAKEAIKRANIT
                     PDMIDESLLGGVLTAGLGQNIARQIALGAGIPVEKPAMTINIVCGSGLRSVSMASQLI
                     ALGDADIMLVGGAENMSMSPYLVPSARYGARMGDAAFVDSMIKDGLSDIFNNYHMGIT
                     AENIAEQWNITREEQDELALASQNKAEKAQAEGKFDEEIVPVVIKGRKGDTVVDKDEY
                     IKPGTTMEKLAKLRPAFKKDGTVTAGNASGINDGAAMLVVMAKEKAEELGIEPLATIV
                     SYGTAGVDPKIMGYGPVPATKKALEAANMTIEDIDLVEANEAFAAQSVAVIRDLNIDM
                     NKVNVNGGAIAIGHPIGCSGARILTTLLYEMKRRDAKTGLATLCIGGGMGTTLIVKR"
     gene            complement(2579..3424)
                     /locus_tag="PC471_003174"
     CDS             complement(2579..3424)
                     /locus_tag="PC471_003174"
                     /EC_number="1.1.1.157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyryl-CoA dehydrogenase"
                     /protein_id="HDF2790282.1"
                     /translation="MKLAVIGSGTMGSGIVQTFASCGHDVCLKSRTQGAIDKCLALLD
                     KNLTKLVTKGKMDEATKAEILSHVSSTTNYEDLKDMDLIIEASVEDMNIKKDVFKLLD
                     ELCKEDTILATNTSSLSITEIASSTKRPDKVIGMHFFNPVPMMKLVEVISGQLTSKVT
                     FDTVFELSKSINKVPVDVSESPGFVVNRILIPMINEAVGIYADGVASKEEIDEAMKLG
                     ANHPMGPLALGDLIGLDVVLAIMNVLYTEFGDTKYRPHPLLAKMVRANQLGRKTKIGF
                     YDYNK"
     gene            complement(3522..4319)
                     /locus_tag="PC471_003175"
     CDS             complement(3522..4319)
                     /locus_tag="PC471_003175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HDF2790283.1"
                     /translation="MSTSDVKVYENVAVEVDGNICTVKMNRPKALNAINSKTLEELYE
                     VFVDINNDETIDVVILTGEGKAFVAGADIAYMKDLDAVAAKDFSILGAKAFGEIENSK
                     KVVIAAVNGFALGGGCELAMACDIRIASAKAKFGQPEVTLGITPGYGGTQRLTRLVGM
                     AKAKELIFTGQVIKADEAEKIGLVNRVVEPDILIEEVEKLAKIIAKNAQLAVRYSKEA
                     IQLGAQTDINTGIDIESNLFGLCFSTKDQKEGMSAFVEKREANFIKG"
     gene            complement(4338..5348)
                     /locus_tag="PC471_003176"
     CDS             complement(4338..5348)
                     /locus_tag="PC471_003176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HDF2790284.1"
                     /translation="MGNVLVVIEQRENVIQTVSLELLGKATEIAKDYDTKVSALLLGS
                     KVEGLIDTLAHYGADEVIVVDDEALAVYTTEPYTKAAYEAIKAADPIVVLFGATSIGR
                     DLAPRVSARIHTGLTADCTGLAVAEDTKLLLMTRPAFGGNIMATIVCKDFRPQMSTVR
                     PGVMKKNEPDETKEAVINRFKVEFNDADKLVQVVQVIKEAKKQVKIEDAKILVSAGRG
                     MGGKENLDILYELAEIIGGEVSGSRATIDAGWLDKARQVGQTGKTVRPDLYIACGISG
                     AIQHIAGMEDAEFIVAINKNPEAPIFKYADVGIVGDVHKVLPELISQLSVAKEKGEVL
                     AN"
     gene            complement(5367..6149)
                     /locus_tag="PC471_003177"
     CDS             complement(5367..6149)
                     /locus_tag="PC471_003177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009524639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HDF2790285.1"
                     /translation="MNIVVCIKQVPDTTEVKLDPNTGTLIRDGVPSIINPDDKAGLEE
                     AIKLKEEMGAHVTVITMGPPQADMALKEALAMGADRGILLTDRAFAGADTWATSSALA
                     GALKNIDFDIIIAGRQAIDGDTAQVGPQIAEHLNLPSITYAEEIKTEGEYVLVKRQFE
                     DCCHDLKVKMPCLITTLKDMNTPRYMKVGRIYDAFENDVVETWTVKDIEVDPSNLGLK
                     GSPTSVFKSFTKSVKPAGTIYNEDAKTSAGIIIDKLKEKYII"
     gene            complement(6208..7344)
                     /locus_tag="PC471_003178"
     CDS             complement(6208..7344)
                     /locus_tag="PC471_003178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HDF2790286.1"
                     /translation="MDLNSKKYQMLKELYVSFAENEVKPLATELDEEERFPYETVEKM
                     AKAGMMGIPYPKEYGGEGGDTVGYIMAVEELSRVCGTTGVILSAHTSLGSWPIYQYGN
                     EEQKQKFLRPLASGEKLGAFGLTEPNAGTDASGQQTTAVLDGDEYILNGSKIFITNAI
                     AGDIYVVMAMTDKSKGNKGISAFIVEKGTPGFSFGVKEKKMGIRGSATSELIFEDCRI
                     PKENLLGKEGQGFKIAMSTLDGGRIGIAAQALGLAQGALDETVKYVKERVQFGRPLSK
                     FQNTQFQLADMEVKVQAARHLVYQAAINKDLGKPYGVEAAMAKLFAAETAMEVTTKAV
                     QLHGGYGYTRDYPVERMMRDAKITEIYEGTSEVQRMVISGKLLK"
     gene            complement(8053..8520)
                     /locus_tag="PC471_003179"
     CDS             complement(8053..8520)
                     /locus_tag="PC471_003179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HDF2790287.1"
                     /translation="MSISNVREYFKQFGKEDSILEFEQSSATVELAAEAAGVIPARIA
                     KTLSFKIGDDAILIVTAGDAKIDNKKYKAEFNCKAKMLTPEEVLEFTGHAIGGVCPFG
                     LKNSIKVYLDDSMKRFDTVFPACGSSNSAIELTCEEMEKFSKCEKWVDVCKNW"
     gene            complement(8599..9141)
                     /locus_tag="PC471_003180"
     CDS             complement(8599..9141)
                     /locus_tag="PC471_003180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2790288.1"
                     /translation="MKKFIKVSFFILGIFTLFIAINKLVYRDMVSVKDYEINHLEEIV
                     FDFDSRRSRDVGTIVRFKISNNSKYSYKLNSAKMKFENCVENKKGMNKCSAYVNVDLF
                     NEKNSITERTLKYGIKPNNQGYVDFVIPKGLNFDYRYFNESGMSVEYKGEYIVNIPMA
                     KGLYLVVGKNNDTWNANIME"
BASE COUNT         2734 a         1807 c          989 g         3685 t
ORIGIN      
        1 attatttgtt cacaacagtt tttaattaaa ttgtcattat aatctttaaa aacacctttt
       61 cttaaatcct catcttttgc aattcttttt atagctttct ggaaaaaacc atccacttct
      121 tgattaactt tgccttcaaa gtcatttaat attggaacat cactattttt gtccaaaaca
      181 tgtattataa atttatgtat tatcatatat atcaccccat tatcttaatt tcgatattaa
      241 ctctttcata tcctctggta tatctgcttt aaatttcaat ttttcttttg ttcttggttg
      301 ttcaaactct agcttatatg catgaagtgc ttgcctattt attagctctt catctacata
      361 accatacaac tcatcaccta ttattccatg acctatatga gccatatgta ctcttatttg
      421 atgtgttcta ccagtttcta agcttacttc taatacagtt gcattttcta gtctttctat
      481 tactttatag tgagtaacag atgactgtcc tctttcatct attatcctct ttattgaatc
      541 ttctgtaggt ctataaattg gctcatctat agttccctca ttttctttta taattccatc
      601 aactactgtt atatactttt tttcaacttt attttcactc attgctgtag acaaagtatg
      661 atgtgcataa gcattttttg ctactataac taaccctgaa gtattcatat ctaacctatt
      721 tacaaatctt atttttacgt tttctttttg attatctata tagtaactaa taccatttgc
      781 tattgtttta tcatagtggc tttttgttgg gtgaactacc ataaacggag gtttattcac
      841 cataattata tcaaaatcat cataaattat ctgtaaattt aaatcttgag gttcaaaatt
      901 tgctttttcc tcgtctattt ttacttctat taaatcacca ctatacacct ttaaactcgg
      961 ttttttatat actccattta ctaaaacact cttttccctc ttcatttttg ataatgacct
     1021 aactgagaaa tttagcttgt ccaataaaac ttcttttaaa gtcatttctt cttcatttgt
     1081 atacgatata agattatatc tttgattctc ttttttaaac aaacttttac ctactttctt
     1141 ttatatcttc ctacttttta tatcttcaaa atcatgatat tatatatttt acactatttt
     1201 tgtttaatag ataataatta tttttaacta taaaaacctg taagaataac aaatagttat
     1261 tcttacaggt taaattcata attaaatatt taatttgtaa atattataat ctaaaactat
     1321 ctcttaacta ttaaagtagt tcccattcct ccacctatac aaagtgtagc aagaccagtt
     1381 ttagcatctc ttctcttcat ttcatataaa agtgtagtaa gtattcttgc tcctgagcat
     1441 cctattggat gtcctatagc tattgctcca ccattaacat taactttatt catatctata
     1501 tttaagtctc ttattacagc tacagattgg gcagcaaatg cctcattagc ttcaactaaa
     1561 tctatatctt caatagtcat attagcagct tctaaagctt ttttagttgc tggaactggt
     1621 ccatatccca ttattttagg gtcaacacca gctgttccat aagaaactat agttgcaaga
     1681 ggctctattc ctagttcttc agctttttct ttagccatta ctactagcat agcagcacca
     1741 tcatttattc ctgatgcatt accagcagta actgttccat cttttttaaa tgcaggtctt
     1801 aacttagcaa gtttctccat tgtagtgcca ggcttaatat attcatcttt atctactaca
     1861 gtgtcacctt ttcttccttt tataacaaca ggaactattt cttcatcaaa ttttccttca
     1921 gcttgagctt tttcagcttt attttgactt gcaagagcta attcatcttg ttcttctcta
     1981 gttatattcc attgctctgc tatgttttca gcagtaatac ccatgtgata gttattaaat
     2041 atgtctgata atccatcttt tatcattgaa tcaacaaaag cagcatcacc cattcttgca
     2101 ccatatctcg cacttggtac taaataagga gacatactca tgttttcagc tccaccaact
     2161 aacattatat cagcatcacc taatgctata agttgagatg ccattgaaac agatcttaat
     2221 ccagaaccac aaactatatt tatagtcata gctggttttt ctactggtat tcctgctcct
     2281 aatgctattt gtcttgctat attttgtcca agacctgctg taagtactcc ccctaaaaga
     2341 gattcatcta tcatatctgg agttatgtta gctcttttta tagcttcttt agctgctgtt
     2401 acccctaact ctaccgctga aactgattta aatgctcctc caaaacttcc tactgctgtt
     2461 ctagctgcac tggcaattac tacttctctc atattcatta cccccattaa attagatttt
     2521 tcatatatcc gaaattgttt tttaaacaat tttatttaaa cttttaattc ccaaattctt
     2581 atttattata atcatagaat cctatcttag tttttcttcc taattgatta gctctaacca
     2641 ttttagctaa aagtggatga ggtctatatt tagtatctcc aaattcagta tataaaacgt
     2701 tcattatagc taaaacaaca tctaatccga ttaaatcacc taatgctagt ggtcccattg
     2761 gatggtttgc tcctaatttc atagcttcat ctatttcttc tttacttgca acaccatctg
     2821 catatatacc aacagcttca tttatcatag gtataagtat tctatttact acaaatccag
     2881 gagattcaga tacatctact ggtactttat tgatactctt agataattca aatactgtat
     2941 caaaagtaac ttttgatgtt aactgaccac ttataacttc aactaatttc atcataggaa
     3001 ctggattaaa gaaatgcatt cctataactt tatctggtct cttagtagaa gaagctattt
     3061 ctgttataga taatgatgaa gtatttgttg ccaagatagt atcttcttta cataattcat
     3121 ctagtaactt gaaaacatct ttctttatat tcatgtcttc tacagatgct tctattatta
     3181 aatccatatc ttttaaatct tcatagttag tagttgaact aacatgactt aatatttctg
     3241 cttttgtagc ttcatccatt tttcccttag taactaactt agttaaattt ttatctaata
     3301 aagctaaaca tttatctata gcaccttgag ttctactctt taaacataca tcatgtccac
     3361 aacttgcaaa agtttgtaca ataccacttc ccatagttcc actacctatt acagctaatt
     3421 tcatagttat aacctcctgt tattttagta tttatattct gttaactaga ttaaaatact
     3481 atttaatcta gttaacagca aatatatttt taattagatt tttacccttt tataaagtta
     3541 gcttctctct tttcaacgaa agctgacatt ccttcttttt ggtctttagt tgaaaaacaa
     3601 agaccaaata aattagattc tatatctatt ccagtattta tatcagtttg agcaccaagt
     3661 tgtattgctt ctttagagta tctaactgca agctgagcat ttttagctat tatcttagct
     3721 aatttctcaa cttcttctat taaaatgtct ggctcaacga ctctatttac tagccctatt
     3781 ttttcagctt catcagcttt tataacttga cctgtaaaga ttaattcttt tgcttttgcc
     3841 attccaacca atcttgtaag tctttgagtt cctccatatc ctggagttat tccaagagtt
     3901 acttctggct gaccaaattt agctttagca gatgcaattc ttatatcaca tgccattgca
     3961 agttcacatc ctccacctaa agcaaatccg tttacagcag ctatcactac ttttttacta
     4021 ttttctattt ctccaaaagc ttttgctcct aagatactaa aatctttagc agctacagca
     4081 tctaaatctt tcatgtatgc aatatctgct ccagctacaa atgcctttcc ttcccctgtc
     4141 aatattacaa catcaatagt ttcatcatta ttaatatcta caaatacttc ataaagttct
     4201 tctaaagtct ttgaatttat tgcattaagg gctttaggtc tattcatttt cactgtacat
     4261 atatttccat ctacttcaac agctacattc tcataaactt taacatcact tgtactcata
     4321 ataccctccc caagaaatta gttagctaaa acttcacctt tttcttttgc aacacttaac
     4381 tgactgataa gttctggaag cactttatga acatctccaa ctataccaac atcagcatat
     4441 ttaaatattg gagcttctgg atttttattt atagcaacta taaactcagc atcttccata
     4501 ccagctatat gttgtattgc tccagatata ccacatgcta tataaaggtc tggtcttaca
     4561 gttttaccag tttgaccaac ttgtcttgct ttatctaacc aacctgcatc tatagtggca
     4621 cgagaaccag aaacttctcc acctataatt tcagctaatt cataaagtat gtctaagttt
     4681 tcttttccac ccattccacg tccagcagaa actaatatct tagcatcttc tattttaact
     4741 tgttttttag cttcttttat tacttgtaca acttgaacta atttatcagc atcattaaat
     4801 tctaccttga aacggttaat tacagcttct ttagtttcat caggttcatt tttcttcata
     4861 acccctggtc taactgtaga catttgaggt ctgaaatctt tacaaactat tgttgccatt
     4921 atatttccac caaaggcagg tcttgtcatt aataataatt ttgtatcttc agctactgca
     4981 agacctgtac agtcagcagt aagacctgta tgtattctag cagaaactct aggtgctaaa
     5041 tctctaccta ttgaagttgc accaaataat acaactatag ggtcagctgc ttttattgct
     5101 tcataagctg cttttgtata tggttcagtt gtatacactg ctaaagcttc atcatctact
     5161 actattacct catctgcacc atagtgtgct aatgtatcta ttaaaccttc taccttacta
     5221 cctaaaagta atgcagaaac ttttgtatca taatcttttg ctatttctgt agcctttcct
     5281 agtaattcta aagaaacagt ttgaattaca ttttctcttt gttctattac tactaaaacg
     5341 ttacccattc tttatccccc cttaaattat atgatatact tctcttttaa tttatctatg
     5401 ataattccag ctgatgtttt cgcatcttca ttgtatattg taccagctgg tttaactgat
     5461 tttgtaaatg atttaaatac actagttgga gaacctttaa gacctaaatt agaagggtca
     5521 acttctatat cttttacagt ccatgtttct actacatcat tttcgaaagc atcatatatt
     5581 cttccaactt tcatgtatct tggtgtgttc atatctttaa gagttgttat aaggcatggc
     5641 attttaactt ttaagtcatg gcaacaatct tcaaattgtc tttttactaa tacatattca
     5701 ccttcagttt ttatttcttc agcatatgtt attgatggaa gatttaaatg ttcagctatt
     5761 tgaggtccaa cttgtgcagt atctccatct atcgcctgtc ttccagctat tataatatca
     5821 aaatctatat tttttaatgc tcctgctaat gctgatgaag ttgcccaagt atcagcacct
     5881 gcaaatgctc tatctgttaa taatatacct ctatctgcac ccattgctaa agcttctttt
     5941 aaagccatat ctgcttgagg aggtcccatt gttataacag ttacatgagc acccatttct
     6001 tcttttaatt ttatagcttc ttctaaacct gctttatcat cagggtttat tatacttggt
     6061 actccatctc taattaaagt acctgtatta ggatctagtt taacttctgt tgtatctgga
     6121 acttgtttta tacaaacgac tatattcata aatccttcct ccatatctat tttaatatcg
     6181 acctattgtg tatattttaa tatttatcta ttttaataat tttcctgaaa taaccattct
     6241 ttgaacttca ctagttcctt catatatttc agttatctta gcatctctca tcattctttc
     6301 tactggatag tcacgagtgt atccatatcc tccatgaagt tgtacagctt ttgtagtaac
     6361 ttccatagct gtttcagctg caaataattt tgccattgct gcttctactc cataaggttt
     6421 tcctaagtct ttatttatag ctgcttgata tacaaggtgt ctagccgctt gtaccttaac
     6481 ttccatatca gctaattgga attgtgtatt ttggaatttt gataatggtc taccaaattg
     6541 tactctttct tttacatatt taacagtttc atcaagagca ccttgtgcta aacctaaagc
     6601 ttgtgcagct ataccaattc taccaccatc aagagtagac attgctatct taaatccttg
     6661 accttctttt ccaagtaaat tttctttagg tattctgcaa tcctcaaata ttaattcact
     6721 cgtagctgaa cctcttatac ccattttctt ttctttaact ccaaagctaa acccaggagt
     6781 tcctttttca actataaatg ctgatattcc tttgttcccc ttagatttat cagtcattgc
     6841 cattactaca tatatgtcac cagctattgc atttgttata aatatttttg agccattaag
     6901 tatgtattca tccccgtcta aaacagcagt tgtttgttgg ccagacgcat ctgtaccagc
     6961 attaggctca gtaagaccaa atgctcctaa tttttctcca cttgctaatg gtcttaagaa
     7021 tttttgtttt tgttcttcat taccatattg atatataggc catgagccaa gagatgtatg
     7081 agctgataat ataactcctg tagtaccaca aactctagac aattcttcaa ctgccattat
     7141 atatcctaca gtgtctccac cttctccacc atattctttt ggatatggta tacccatcat
     7201 tcctgctttt gccatttttt ccactgtttc ataaggaaat ctttcttctt catcaagttc
     7261 tgttgctaaa ggtttaactt cattttcagc gaagcttaca tatagctctt taagcatctg
     7321 atatttttta gaatttaaat ccataatagc ctcctcagta ttttataaat attttatttt
     7381 catttattaa ttaaaatctc ggcaacatct ataataaggt atttgaatat acgtcgagaa
     7441 atttataacc ttaatcaaac tacgctctat atttatatgt gattatacta accaattatg
     7501 acattaattt aatcataaat tgttaattta aaatcaacta tacttaaaaa aaatctcgct
     7561 ttttggaatt ttgtgaaatt tcaagttaga ataagatttc tttcacactg gttagagata
     7621 aaataatttg tttacgatat ttatattaag gtatattaaa cattaaatat tgtctgtaca
     7681 tctgtttttt agccatgtac agaagatttt tatcgtataa aaattatttt aatagagcta
     7741 aataatcata tttttatctg actatttttt atttttaaat atcttacatc tataatagta
     7801 tcactcaaag gataattttt caataatttc taattatttt tttaacaata tttattacat
     7861 tttttccact tttttgtcat ttttttattg tttcgaaaaa aaatcagaat tttttaactt
     7921 tattttggat tattagttta aattagattt tcctcttaga tttataaata tttacacaaa
     7981 aaaactacct ctgtatagat tgtatacaga ggtagttttt tactaattcc tactatagtt
     8041 tactatattt ttttaccaat tcttacaaac atctacccat ttttcacact tagaaaactt
     8101 ctccatttct tcacatgtaa gttctattgc agagttacta ctgccacatg caggaaatac
     8161 tgtatcaaat cttttcattg aatcatctaa atagactttt atagaatttt ttaaaccaaa
     8221 tggacagact ccaccaatag catgtccagt aaactccaag acttcttctg gtgtcaacat
     8281 tttagcctta caattaaatt cagccttata cttcttatta tcaatcttag catcaccagc
     8341 tgtcacaatt aaaattgcat catctcctat tttaaatgag agtgtcttag ctattcttgc
     8401 tggaataacc ccagctgctt cagctgcaag ttccacagta gcactagatt gttcaaattc
     8461 taatatactg tcttccttgc caaattgttt aaaatactct cttacattac ttattgacat
     8521 taaatacccc tcctagtctg aacttttata aatttcatat tattcttaat tatatctata
     8581 attgatttat ttttcaaatt attccataat attagcattc catgtatcat tattttttcc
     8641 aactactaga taaagtcctt ttgccattgg aatattgaca atatattctc ctttatactc
     8701 tacactcata ccagattcat taaagtacct ataatcaaaa tttagtcctt ttggaattac
     8761 aaaatcaaca taaccttgat tatttggttt tattccatat ttcagagttc tttcagttat
     8821 agaatttttt tcattaaata aatctacatt tacatatgca gaacacttat tcattccctt
     8881 tttattttcc acacaatttt caaacttcat tttagcactg tttaatttat aactatattt
     8941 actattattg cttattttaa atcttacaat agtaccaaca tctctacttc tcctgctatc
     9001 aaaatcaaaa acaatttcct ctaaatgatt tatttcataa tctttgacac ttaccatatc
     9061 tctatatact aatttattta tagctatgaa tagtgtaaaa atccccaaaa taaaaaatga
     9121 aacttttata aacttcttca tttttagtct ccttatattt tgtgctattg tgctataata
     9181 ttaatcaaaa ttttaataat taaacttatt ctttt
//
LOCUS       DAMJYR010000138         9170 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.143, whole genome shotgun sequence.
ACCESSION   DAMJYR010000138 DAMJYR010000000
VERSION     DAMJYR010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9170)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9170)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9170
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.143"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            114..497
                     /locus_tag="PC471_003181"
     CDS             114..497
                     /locus_tag="PC471_003181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2790289.1"
                     /translation="MSTDKVVGIIDEETAELAGIEYTGKIYASSGVIKHIKKKHRCQL
                     SKDIFNDIIDTIKMVLKSPEYIGSHPKKPGKSVEFIKKIDDYILVATELDTKKGYLYV
                     SSMYAIKEAKLESRVNSGRVKQYNG"
     gene            1005..1388
                     /locus_tag="PC471_003182"
     CDS             1005..1388
                     /locus_tag="PC471_003182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HDF2790290.1"
                     /translation="MNLSKKIEDKRKAKQRAEKVKKAKIATAGVVLGAVTGAVSGVLL
                     APKSGKETREDIKDASQQIAEKINMKTVDVKGKVSEKLEDKKGNFIESKKKIKKYIDN
                     KKSVENSSEEDKIIDIVEPVSVTEE"
     gene            1390..1740
                     /locus_tag="PC471_003183"
     CDS             1390..1740
                     /locus_tag="PC471_003183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2790291.1"
                     /translation="MDNTITINISIIYVLLAILACIALVYLIIVLVKLHKILNNVGTM
                     LNENTQNINTTLNNLPNVVETFGEVGENMKDVTEVVTEVTADFLVTKDSVKSNMDVIV
                     DILTIIKNIFLDKK"
     gene            complement(1841..2299)
                     /locus_tag="PC471_003184"
     CDS             complement(1841..2299)
                     /locus_tag="PC471_003184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2790292.1"
                     /translation="MIRTKVVELIATVCRENKPHKWVDENYTPYDKSGKVELMSIEDL
                     NELISSSGRADFLYSSRLQKLLEEVYINQSRASYISGCGLFWSSYWDILEEKFEEWLY
                     NSYIFFDEDDEYLEGMEDFELECKDVLMDVIETTSIDIYVQMIKRNITNY"
     gene            complement(2344..3132)
                     /locus_tag="PC471_003185"
     CDS             complement(2344..3132)
                     /locus_tag="PC471_003185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HDF2790293.1"
                     /translation="MKVVIIPGHTLIGKGTGAVGYINESKETRILNDLIVKWLKIGGA
                     TVYTGRVDESSNHLADQCAIANKQETDLAVQIHFNSNATTSTPVGTETIYKTNNGKTY
                     AERVNTRLATVFKDRGAKSDVRGLYWLNHTIAPAILIEVCFVDSKADTDYYVNNKDKV
                     AKLIAEGILNKSISNSQEGGENKVYENVIVYTGDADKVAAQILHWQLKDSLIIEASSY
                     KQGLGKKVYVVGGEANKLVKGDVVINGADRYETVKLALQEIDKL"
     gene            complement(3271..3708)
                     /locus_tag="PC471_003186"
     CDS             complement(3271..3708)
                     /locus_tag="PC471_003186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine/deoxycytidylate deaminase family
                     protein"
                     /protein_id="HDF2790294.1"
                     /translation="MNKKLENRCSWQEYFMRLCETVAERGTCDRAYVGAIIVNSENRI
                     VSTGYNGSISGDKHCSEVGHEMRDGHCIRTIHAEQNALYYCAKEGISVKNCSIYVTHF
                     PCLNCTKAIIQAGIKHIYYRTGYRIDEYAIKLLQSSNVLYTKL"
     gene            4530..5039
                     /locus_tag="PC471_003187"
     CDS             4530..5039
                     /locus_tag="PC471_003187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2790295.1"
                     /translation="MLSENIKTIRKSKGLSQQELAIKLNVVRQTISKWEQGLSVPDSD
                     MLISISEVLETPVSTLLGETIVESEVDNLKVISEKLEIINLQLAQRKNMRRKMFHWLF
                     ILLCAVILIVFVVLGGLNSPYLDWNYTDPETAVFGVAFHAFEWLFVRLVPIVLIGAII
                     GIFLTRKKI"
     gene            5445..5981
                     /locus_tag="PC471_003188"
     CDS             5445..5981
                     /locus_tag="PC471_003188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HDF2790296.1"
                     /translation="MQGGVNIRQVKSFVFPVISLIFLDQISKVLIGLFLMDFEIDIIG
                     KFLRFNPVQNTNLSYGGNFIGILSNLWVLVLFNILVILVIISGYAFYKSKNEQTSYSV
                     KVIMSCGLAGTICSLIDKLFWGGSLDFLQIPSFFIFDLKDCYLTVAEIIFVVIGILHN
                     REISMKEYIYFCYRQFKK"
     gene            complement(6408..7208)
                     /locus_tag="PC471_003189"
     CDS             complement(6408..7208)
                     /locus_tag="PC471_003189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2790297.1"
                     /translation="MRLNKRSEGYINYVKGVKREKRNVLFYQLLILIGFIVIWELLAD
                     LNVINTFLFSKPSDIYNLFIQYAFSGQLFKHIGISVYETVLGLVIGTVLGILVAIALW
                     WSEKLSKILDPFLVVLNALPKTALAPIIIVWVGAGIEGIVVTAVTISVVVTILSAYNY
                     FMNIDEEKIKMLKSFGATKSQILFKLILPANTGNLINLTKINIGMAWVGVIVGEFLVS
                     RYGIGYLIVYGSQVFKLDLVMMGVFVLAICAWAMYAVVNIIEKIYNSR"
     gene            complement(7403..9001)
                     /locus_tag="PC471_003190"
     CDS             complement(7403..9001)
                     /locus_tag="PC471_003190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDF2790298.1"
                     /translation="MKAAIYSRKSRFTGKGDSIENQIQLCIDYAKSIGVKDFLIYEDE
                     GFSGSNIDRPQFKKMMQDAKDKKFSYLVCYRLDRISRNVSDFSTLIEKLNELSISFIS
                     IKEQFDTSTPMGRAMMFISSVFAQLERETIAERIKDNMYELARSGRWLGGNTPHGFNS
                     EKITFLDENLKERSMYKLQVNDEQMEIVKLIYNKYLELRSLSKLYKYVYNNGIRGPRG
                     GKFDPSSLSRILRNPAYVKANDEILDYLKNSGMDVVGTPDRKCGILTYAKNTSNSIAA
                     IAKHHGVIEPEIWLEVQNQLDKNRDKTPRISTGKTALLSGLIKCGKCGSNMRITYKGK
                     KTDKDLKYYYICGTKKTLGSDSCNCKNLNGPLVESLVIDKIKNCKEESIISAFNSNNI
                     KTDNVFNIFKSETNLLKNQILDKERLIKNLVIELAKNTGSTASDYIVSQIESLNNEIS
                     ILKDKINTLESSNINISSDSINIDIIVSNLKRFNDEVDNASLDDKRLLLSTIINSIVW
                     DSSLGNLNIVYMGAEVDNSVSLSN"
BASE COUNT         3297 a         1346 c         1177 g         3350 t
ORIGIN      
        1 atattatact atcttttctt tttggaataa actatacttt aaatatagat atttaattta
       61 aataaatttt acagtattaa tagtaaatta tcttagaaag gaatgtgctt tatatgtcta
      121 cagacaaagt tgttggaatc atagatgaag aaacggcaga acttgcagga attgaatata
      181 ctggtaaaat ttatgcttct tctggtgtta taaaacatat aaagaaaaaa catagatgtc
      241 aactgagtaa ggatattttt aatgatataa tagacactat aaaaatggta ttaaagtcac
      301 ctgaatatat aggaagtcat cctaaaaaac ctggaaaaag tgtggagttt ataaaaaaaa
      361 ttgatgatta tattttagta gctacagaat tagatacaaa aaaaggatat ttatatgtat
      421 cttcaatgta tgctataaaa gaagcaaaat tagaaagtag agtaaacagt ggaagagtaa
      481 aacaatataa tggttaacat aatattatta attttcccta aaatattgaa aaatattatt
      541 atataagtta taatattaat atacacgtag atatttatta gttagatttg aggttggaaa
      601 aggcacccaa tacgctacta tagagtagtt aatagagatg caggatacgc cgccctgcca
      661 aatctaatta aattaaaaag agaggattaa ttcctctctt attgtttttt tgagatacta
      721 atttaatcta taatagaaag attgaaggtc gtttatacaa aaaaattttc agttatatac
      781 attaaactaa ttaaataaga tatttataat ctatatgtga tacatattat atattaataa
      841 aagaaaaata tatagaaaat ccttataaaa atttaaattg taatgaatag gaagtaatta
      901 aaaagaattt ttattgattt ttaaaaattt agttagaaac tatatcatta tggtaaaaaa
      961 tgtattataa tgtttagaaa tattataata aggagcgatt aaacatgaat ctatcgaaga
     1021 aaatagaaga taaaagaaaa gctaaacaga gagctgaaaa agttaagaaa gctaaaattg
     1081 ctacagctgg tgtggtatta ggagctgtta ctggtgcagt gagtggagta cttttggctc
     1141 ctaaatctgg aaaagaaaca agagaggata taaaagatgc ttcacaacaa attgctgaaa
     1201 agataaatat gaaaactgta gatgtaaaag gcaaagtatc tgagaagcta gaggataaaa
     1261 aaggaaactt tatagaatca aagaaaaaaa taaaaaaata tatagataac aaaaagagtg
     1321 ttgaaaattc tagtgaggaa gataaaataa tagatatagt tgaaccagtt agtgttactg
     1381 aggaataata tggacaatac aataacgatt aacataagta taatatatgt attattagct
     1441 atacttgctt gtattgcttt agtttattta attattgtac ttgttaaact acataagatt
     1501 ttaaataatg ttggaactat gttaaatgaa aatactcaaa atataaatac aactttaaat
     1561 aatcttccta atgttgtaga aacttttgga gaagtaggag aaaatatgaa agatgtaaca
     1621 gaagtagtta ctgaagttac agcagatttt cttgttacta aagacagtgt caaatcaaat
     1681 atggatgtta tagttgatat attgactata attaaaaaca tttttctaga taaaaaataa
     1741 taagtttaat taaaataagc ctataagata taatttaaac gaccttaaaa tagcatttat
     1801 aaggtcgttt tttattagct tgatttgaca ctatgtgttt ttaataattg gtaatattcc
     1861 tctttatcat ttgaacatat atatctatgc ttgttgtttc aattacatcc ataagaacat
     1921 ctttacattc aagctcaaaa tcttccattc cttctaaata ttcatcatct tcatcaaaaa
     1981 agatataaga attatatagc cattcttcaa atttttcttc aagtatatcc caatacgaac
     2041 tccaaaataa tccacaacct gatatataag atgctctact ttgatttata taaacctcct
     2101 ctagaagctt ttgtaatcta ctgctataaa gaaaatctgc tcttccactg cttgatatta
     2161 attcatttaa atcttctatt gacattaatt caactttacc acttttatca taaggagtat
     2221 aattttcatc aacccattta tgtggtttgt tttctctgca tactgttgct atcaactcta
     2281 caacttttgt tcttatcatt tttttatcct aacttattta ttttttataa aataatcatg
     2341 attttataat ttatctattt cttgtaatgc taatttaaca gtttcatatc tatcagcacc
     2401 atttattact acatcacctt ttacaagttt attagcttct ccacctacaa cataaacctt
     2461 tttacctaaa ccttgcttat aactagacgc ttcaattatt aaagaatctt tcaattgcca
     2521 atgtaaaatc tgagcagcca ctttatcagc atcacctgta taaacgatta cattttcata
     2581 cactttattt tcccctccct cttgagaatt tgatatagat ttgtttagta taccttctgc
     2641 tatcaattta gcaactttat ctttattatt aacataataa tctgtatctg ctttactatc
     2701 tacaaaacat acttctatta atattgctgg tgcaatagta tgattaagcc aataaagacc
     2761 tctaacatca gactttgcgc ccctatcttt gaatactgta gctaacctag tattcactct
     2821 ttctgcatag gttttgccat tattagtctt atatattgtt tctgtcccta ctggagttga
     2881 agttgtggca tttgaattaa aatgaatctg aacagccaaa tcagtttcct gtttatttgc
     2941 tatagcacat tggtctgcta aatgattact tgattcatca actcttcctg tataaacagt
     3001 agctccacct attttaagcc atttaacaat taagtcattc agtattcttg tttctttgct
     3061 ttcgtttata tacccaacag ctcctgtacc ttttccaatt aaagtgtgcc ctggtattat
     3121 tactactttc aaactaacat ctcctttaca attaaattta acttctattt aatatatgca
     3181 taacctagaa ttttgtttct ttttatatct tatactttta ttataaccca ttttgtaaaa
     3241 atatagaata ttaagtaaat aatgttaaaa ttataatttt gtatataaga cattacttga
     3301 ttgcaataat tttatagcat attcatctat tctatatcca gttctataat atatatgttt
     3361 aattccagct tgaattatag ccttagtaca atttaagcaa ggaaagtgag ttacataaat
     3421 agagcaattt ttaactgaaa taccttcttt tgcacaataa taaagggcat tttgttcagc
     3481 gtgaatggtt ctaatacaat gtccatctct catttcatga ccaacttcac tacaatgctt
     3541 atctccagaa atactaccat tatatccagt agacactatt ctgttttctg aattgacaat
     3601 tatagctcct acataagctc tatcacaagt tcctctttca gctactgttt cgcacagtct
     3661 cataaaatat tcttgccatg agcatctatt ttcaagtttt ttattcaaat tatatacctc
     3721 caaaacttat atctaatata taagatttat accttaaaaa taatattaaa aacaaattta
     3781 tacgataaat aaaatttatt aatactgttt ttagtctaaa ttatttcatt ttaaattaaa
     3841 gttacaagga atctaaaatt atatatatct ttcttcgtaa tattaatgtt acaatataaa
     3901 aaactgtctc aataaaatag agacagtttt acagtcaaag tttataatac aagttcatat
     3961 ttttctttaa attcttttac agcttcgggc catgcctttt gttctgtaat acctttttct
     4021 ttagctattt taattgcaat ctctcttatc attgcagcgc cttctaatat tcccattttt
     4081 aatctccctt caaatatctt tttgtataga atataaatag tgctaagatt tattacatgc
     4141 tgcatataca atatctatac aacacataac ttgtaaattc cttttatttt catacgtctt
     4201 taaccgacat ataattatat tcaataagtt ttaaaattat gaactcaatt ataattattt
     4261 ttattttttt agaatctaaa cactaaaaat tgtaggtaaa tttgtcttat gcctttataa
     4321 atatttctac agttttaatt actggacata tctattttta ttataaaaag cgattaaaaa
     4381 tcgagaatat ccgcataaaa actgtatata agcaaaacgg ttaaaggaaa aagaagaaat
     4441 ttacttgaat gttaaaatgc gttgcttgaa gcaatgggca gttttgccta taatatcggt
     4501 aacaactgaa agaaaggtgg ttagccctaa tgttaagcga aaacattaaa acaatcagga
     4561 agtcaaaagg actttcacaa caagaacttg ctataaaact gaatgtagtg cgacaaacaa
     4621 tatctaaatg ggaacaagga ctgtcagttc ccgattctga tatgttgatt tccatatcag
     4681 aggtgcttga aacgcccgta agtactttac ttggagaaac tattgttgag tcggaagttg
     4741 ataacttaaa agtgatttct gaaaaattag agattataaa cttgcaactt gcacaaagaa
     4801 aaaacatgag aagaaaaatg tttcattggt tatttatttt attatgtgca gtcatattaa
     4861 tagtttttgt ggtcttaggg ggactaaata gtccttactt ggattggaat tataccgacc
     4921 ctgaaaccgc tgtttttgga gtagcttttc atgcttttga gtggctattt gtcagattag
     4981 taccgattgt ccttatagga gcaattattg gaatcttttt gacacgtaag aaaatatgaa
     5041 gtaactctgc tttttataat acagcaagcg ttccttaaaa gcgtcattct acatggcaat
     5101 cgaggacgac tatgtaagga agaacctttt tgatttccaa ctaagcgaag ttattgagga
     5161 cgacagtaag accaaaaccg cattaagtaa agtacaggaa gcaagactgc tggacttata
     5221 ctctcatgcg tcagatgcag gaaatgcgag gtctggtagc atgatttaca acgatttgta
     5281 caacgactgc tagtagaaat aatatggaga taagtattat ctacaaagtc agaaaagaaa
     5341 tgacttgata atttactcat gttataatta ttgatgtgaa attaataaat cgaaattcct
     5401 tttttggatt taataatact gagtaaggaa aaagtgaatt acttttgcaa ggaggtgtta
     5461 atatcaggca agtaaagtct tttgtttttc cagttatatc cttaatattc ttagaccaaa
     5521 ttagcaaagt tcttatagga ttattcttaa tggactttga aattgatata attgggaaat
     5581 ttttaagatt caatcctgtt caaaatacaa atctctctta tgggggaaac tttattggta
     5641 ttctatctaa tttatgggta ttggtattgt ttaacatttt agttatatta gttattatat
     5701 ctggatatgc tttttataaa tcaaaaaatg aacaaacaag ctattcagta aaagtaatta
     5761 tgtcttgtgg acttgctggt acaatatgta gcttgataga taaattattt tggggaggaa
     5821 gtttagattt tttgcagata ccaagctttt ttattttcga cttaaaagac tgctacctta
     5881 ctgttgcaga aataatattt gttgtcatag gaattttgca taatagagaa atatcaatga
     5941 aagaatacat atatttttgc tatcgtcagt ttaaaaaata aatttagaag ataaatttaa
     6001 gtttcaaggt aaacttattt aatcacagca aatagttggc ttacttaagc ttttgggcta
     6061 aactgtgagc actaattaag tctctgatat ctaacaaacg gagcacaaaa ataaaaatta
     6121 aatataaagc acagttaatt ttgattaaaa agttgactgt cttttctttt gtagaaattt
     6181 aagcataggt aataaagaaa tgactgcatt taagcattat gtatataatt ggaggcaata
     6241 aaaagccagc aaagcccata aaatagagct gtgctagcaa catgatgaat tataagaaga
     6301 taatcaaaaa cttacgcttt attttaattt aaaaaaatga ttactgtaaa gaaatcattt
     6361 tttattataa tcactgatac atcaaatatt ctaaataaaa tttttattta tctggaattg
     6421 tatatttttt ctataatatt aactacagcg tacattgccc aagcacagat agccaataca
     6481 aatactccca tcattactaa atctaattta aatacttgag aaccatatac tatcaagtag
     6541 cctattccat atcttgaaac taaaaattct ccaactatta cacctaccca tgccatacca
     6601 atattaattt tagttaaatt tattaaattt cctgtgtttg caggtaatat gagtttaaat
     6661 aatatttgag atttagtagc tccaaaactt tttaacatct taattttttc ttcatctata
     6721 ttcataaaat aattataagc tgaaagtata gtaacaacaa cagaaatcgt tacagctgtt
     6781 acgacgattc cttctattcc agcacctacc caaactataa ttataggagc taaagcagtc
     6841 ttaggaagag catttaaaac taccaaaaat gggtctaata tttttgataa tttttctgac
     6901 caccaaagag caattgctac caaaatacct aatacagtac ctataacaag accaagaaca
     6961 gtttcatata cagatattcc aatatgttta aataattgac cactaaatgc atattgaata
     7021 aataaattgt aaatatcact tggtttgcta aatagaaatg tatttataac atttaagtca
     7081 gctaaaagtt cccagattac aataaaacca attaaaatta atagctggta gaataataca
     7141 tttctttttt ctctttttac acctttaaca tagtttatat atccttcaga tcttttatta
     7201 agcctcatgt gtatctagct ccttccataa tatgtcgaaa tacttttgaa aatcaggaac
     7261 tttacgtgtt ttgagaggcg tttttgttcc atccatgtta agttcaatat cgtaaatatt
     7321 ttttatggta gctggtcttt tagatagtat tgcaacctta tctgatatgc ttattgcttc
     7381 tgctatagct aaaaaagtgc aactaattgc ttaaagaaac agaattatca acttcagcac
     7441 ccatataaac aatattaaga tttcctaaac tgctatccca aacgatacta ttaataatag
     7501 tagataataa caatctctta tcatctaaac tggcattatc aacttcatca ttaaatctct
     7561 tcaaattact aacaataata tcaatattaa tactgtcact actaatattg atgttactag
     7621 attccaaagt attaatctta tcctttaaaa tactaatctc attatttagg ctctcaatct
     7681 gggaaactat ataatcagaa gcagtactac cagtattttt tgcaagctct ataactaaat
     7741 ttttaataag tctttcctta tctaaaattt gatttttaag taaattagtc tcagatttaa
     7801 aaatattaaa aacattatca gtcttaatgt tattactatt gaatgcactt ataatagact
     7861 cttctttaca attcttaatt ttatctataa ctaaactttc aactaatgga ccattaagat
     7921 ttttacagtt gcaactatca gacccaagtg tttttttagt accacaaata taataatact
     7981 ttaaatcctt gtcagttttt ttgcctttat aggtaattct catattagaa ccacatttac
     8041 cacattttat aagaccagat aaaagagcag tttttccagt tgatatcctt ggagttttat
     8101 cacgattttt atctaattga ttttgtactt caagccatat ttcaggttct atgactccat
     8161 ggtgttttgc aattgctgct attgagttag atgtattttt agcataggta aggattccac
     8221 attttctatc tggagtacct acaacatcca taccactatt tttaaggtaa tctaaaattt
     8281 catcattagc ttttacatat gctggatttc taagtattct agataaagaa ctagggtcaa
     8341 atttgccacc acgaggaccc cttattccat tattatagac atatttatat aacttagata
     8401 aggacctaag ttctaaatat ttattgtata ttagctttac aatctccatt tgttcatcat
     8461 ttacttgaag cttatacata cttctttctt ttagattttc atctaagaat gtgatttttt
     8521 cactgttgaa tccatgaggt gtattgcctc caagccatct accactacgg gctaattcat
     8581 acatattgtc ttttatacgt tctgctattg tttctctttc taattgagca aatacacttg
     8641 atataaacat catagctctt cccattggag ttgaagtatc aaattgttct ttgatagata
     8701 taaatgatat gcttagttca tttaattttt ctattaaagt agagaagtca gatacatttc
     8761 tcgatatcct atctaatcta taacaaacta ggtaactaaa tttcttatct ttggcatcct
     8821 gcatcatttt cttaaattgt ggtctatcta tattgctacc actaaatccc tcatcttcat
     8881 atatcaaaaa atcttttaca ccaatagact ttgcataatc aatacataat tgtatttgat
     8941 tttctataga atcacctttt ccagtaaatc ttgattttct tgaataaatt gcagctttca
     9001 aaattaaaac cccctataag ttaggatata tatttgaaac tatttatcta caacctttta
     9061 agtaaactta ttagaatgaa tttattaatt aggtaaattt atataattct actttttata
     9121 atatatgact atttatttta tttctttact attcaagttc taaatcaata
//
LOCUS       DAMJYR010000139         8976 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.144, whole genome shotgun sequence.
ACCESSION   DAMJYR010000139 DAMJYR010000000
VERSION     DAMJYR010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8976)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8976)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8976
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.144"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            177..1382
                     /locus_tag="PC471_003191"
     CDS             177..1382
                     /locus_tag="PC471_003191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HDF2790299.1"
                     /translation="MSKKVLIMSASTGGGHNRAALAIKEELTSKTLDGEPIECEIIDS
                     LKLVNNTMDKIISRGYEKSAIYTPKAYGSVYRLSETNLLSKNEFKDNLLITFMAKKFK
                     KLIRSEKPDLIIGTHPFPMIALSTLKKNFNLHNNESNAYTEHFYKHYTNTINVPPLIS
                     VLTDYTTHSTWIQNEIDYYIVGHEYVKELLVFDGVEPSKIRTFGIPVEKSFLSHRDKG
                     IVLSELNLSPDKLTVLLMGGSFGAGNIKETLDELLDTDRDFQILVITGKNESLKEKIE
                     KKLMSRYHDKNVCVLGYTDKMNDILASIDVLVSKPGGLTTTEALLKDVPMIVPYYIPG
                     QEEENLDFLSNCGAALRTTKKYNLSILLKVLIDDPSRLEMLKRNIKSIRKSNSAQNIA
                     NLILNIISG"
     gene            complement(1453..1674)
                     /locus_tag="PC471_003192"
     CDS             complement(1453..1674)
                     /locus_tag="PC471_003192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2790300.1"
                     /translation="MKPIMNLEEHNAMKEKRVKKMKSKDKPKYDDFEDISFSNKKKTK
                     KNVRNKNAEFRNNYKNYQYDYEEDFYEPI"
     gene            complement(1815..2414)
                     /gene="recR"
                     /locus_tag="PC471_003193"
     CDS             complement(1815..2414)
                     /gene="recR"
                     /locus_tag="PC471_003193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012634696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HDF2790301.1"
                     /translation="MQVYTGPITRLIEEFSKLPGVGRKTAQRLAFHIINMNTNDVEAL
                     SKAIIDAKREIRYCSICCNITDTDPCSMCSNKSRDSSVICVVEDPRDVAAMERTREFK
                     GQYHVLNGVISPMDGIGPDMLKIKELIQRLGNQEVKEIIMATNPTIEGEATAMYIARL
                     VKPMGIKVTRIAHGLPVGGDLEYADEVTISKALEGRREI"
     gene            complement(2427..2777)
                     /locus_tag="PC471_003194"
     CDS             complement(2427..2777)
                     /locus_tag="PC471_003194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HDF2790302.1"
                     /translation="MAKKGFGGGMMPGGGNMNNLLKQAQKMQENMQKAQQELESKEVE
                     ASVGGGAVTVKVNGKKEVIDITIKPEVVDPDDIEMLQDLVLSAVNQALRNIDDIQASQ
                     MSKVTGGMNIPGLF"
     gene            complement(2836..4473)
                     /gene="dnaX"
                     /locus_tag="PC471_003195"
     CDS             complement(2836..4473)
                     /gene="dnaX"
                     /locus_tag="PC471_003195"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HDF2790303.1"
                     /translation="MHKALYRAYRPQKFEDVIGQDHIIKTLKNQIYSDNIGHAYLFCG
                     TRGTGKTSTAKIFSRAVNCLNKINEEPCNECEICESVLKDNTMDVVEIDAASNNSVDD
                     IRELRESVKYSPANAKYKVYIIDEVHMLSQGAFNALLKTLEEPPSYVIFILATTEPHK
                     IPATILSRCQRYDFKRVTVKDMTLRMKKICEDEGIDIDDKALNLIARNSQGALRDALS
                     ILDQCMSFGESKIDYKDVVELMGSVNIEQLFELSQCIVEQDTKKSLEILNEFVLWGKD
                     IRNLINDLIDHFRNLMVCKVSSELDEIISLPEETIEQLKIQSKNIDINDLIRILNILS
                     ITQDDIKSSSNPRVLVEITIMKIAQPMFDESKEALIKRVENLEKMIELGNFKSEKIGN
                     NKEKEYEVDREIDVKQENVVYEDVKNEDVILIESSWKNILKQIKKDKKMPIYALLSEV
                     KSFNVYSNMLYVIFDDKFDFAKTRLSSQDTINYLEKTIRDVLNRSFNVKIVLTSEVKD
                     INLEVKEKKDIGEEILKNIVSEEILEIKDSIDENESK"
     gene            complement(4550..4814)
                     /gene="ffs"
                     /locus_tag="PC471_003196"
     ncRNA           complement(4550..4814)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="PC471_003196"
                     /product="signal recognition particle sRNA large type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01854"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01854"
     gene            complement(4852..4943)
                     /locus_tag="PC471_003197"
     tRNA            complement(4852..4943)
                     /locus_tag="PC471_003197"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4907..4909),aa:Ser,seq:gga)
     gene            complement(4944..5399)
                     /locus_tag="PC471_003198"
     CDS             complement(4944..5399)
                     /locus_tag="PC471_003198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HDF2790304.1"
                     /translation="MESSFYMKEALKEAYKAYNKKETPIGAIIVKDNQIIARAHNLTE
                     TLKDSTAHAEILAIKQASEKLGGWRLTDCDLYVTMEPCIMCSGAIVNSRIKKLIIGTR
                     HVKNSYIEKQHEFKLDYFNNNNVKVAFDVLQEECSIILQEFFKALRKRD"
     gene            complement(5720..6991)
                     /gene="serS"
                     /locus_tag="PC471_003199"
     CDS             complement(5720..6991)
                     /gene="serS"
                     /locus_tag="PC471_003199"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000884181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HDF2790305.1"
                     /translation="MLDIKRIRENLDDIKKAMERRGEREFDLDAVVELDNKRREILQE
                     VEVMKNELNVNSKKIPQLIKEGKDVTEEKARLKELSDKIKGIDEKVKEVEAKMEYTLM
                     RIPNVPHPEVPQGETDEDNVQIRTWGEPTKFDFEHKAHWDIGTGLGILDFERAGKITG
                     SRFTLYRGLGARLERSLMNFFLNTHTAKHGYTEVLPPFMANRNSFIGTGQLPKFEEDM
                     FKIEGLEYFLIPTAEVPVTNIHANEILDVAELPIKYCAYTPCFRSEAGSAGRDTRGLV
                     RQHQFNKVELVKFVKPEDSYNELESLTHDAETMLQMLGLPYRVVKICTGDLGFTAAFK
                     YDLEVWMPSYNRYVEISSCSNFEDFQARRAGIRFKRDKKSKAEYVHTLNGSGLAIGRC
                     LAAILENYQQADGSVVVPEELRPYMGVDVIK"
     misc_binding    complement(7117..7322)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(7450..8331)
                     /locus_tag="PC471_003200"
     CDS             complement(7450..8331)
                     /locus_tag="PC471_003200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HDF2790306.1"
                     /translation="MNFLSLAKTASTNSNITKRTLENMLDNFSDNIPNIIYAIIVFVI
                     GIYISKIVRRMVSKFLIKYGMSKGVNNFIVYGIYISMLSIISLISLGIIGIQTTSVVA
                     VLGAAGFSIGLAFKEILSNLGSGMIILFFKPFNIGDYIQGSGVEGTVSDIQIFSTVLK
                     TPDNKTIIIPNFQLTSNNIINYTHQNKRRIDFSYNISYDSDIDVVKSILNEIFTNEKR
                     ILNDPKPIIGLNSIGNNTMQIVARPWVKTDDYWDVYFDVMEKVKNKFDENDIKVPFVP
                     SSLLFNNTGNLNTMDKR"
     gene            complement(8343..8852)
                     /locus_tag="PC471_003201"
     CDS             complement(8343..8852)
                     /locus_tag="PC471_003201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HDF2790307.1"
                     /translation="MNSNERRSKLIDILKESKHPVKGGTLAELLNVSRQVIVQDIALI
                     RARGFEIIATPQGYIIYNQPYFTKQIKCKNHKDSKEIYDELKIIVDLGGIIKDVIVNH
                     PTYGQITAELNIYSKMDIDNFMKKVETNEFKQLSVLTECSHIHTIEAIKEETIEAIVK
                     ELKDNGILS"
BASE COUNT         2878 a         1573 c          963 g         3562 t
ORIGIN      
        1 atttttaata taacatattt gataaaatta atttacattt ctttcacttg taatttttgc
       61 aatcttgtat tagattaata ttaaaaataa tttatcaata accttattca caaagacagt
      121 ttttatatat caaaatataa tataaatact atgctacatt ttaggaggat tttattatga
      181 gcaaaaaagt attaataatg tctgcttcca ctggtggtgg gcataatagg gctgctctag
      241 ctattaaaga agaacttact tccaaaacac ttgatggaga gccaattgaa tgtgaaataa
      301 tagatagttt gaaattagtt aataatacaa tggataaaat aatttcaaga ggatacgaaa
      361 aatctgctat atatactccc aaagcctatg gaagtgtata tagattatct gaaacaaatc
      421 ttttatcaaa aaatgaattt aaagataatc tactaataac attcatggct aaaaaattta
      481 aaaaacttat tagaagcgaa aaaccagact tgattatagg aacgcatcct tttccaatga
      541 ttgctctaag tacattgaaa aaaaacttta atttacataa taatgaatca aatgcttaca
      601 cagagcattt ttataaacat tatacaaata ccattaatgt tcctccgctt atttctgtat
      661 taaccgatta tactactcat tctacatgga tacaaaatga aattgattac tatattgttg
      721 gtcatgaata tgtaaaagaa ttattggtgt ttgatggagt tgaaccgagt aaaattagaa
      781 catttggaat ccctgttgaa aaatcttttc tctctcacag agataaaggt attgttcttt
      841 ctgagttgaa tttatctcct gataaattaa ctgttttact tatggggggt agctttgggg
      901 ctggaaatat aaaagagaca cttgatgaat tactggatac tgatagagac tttcaaatac
      961 ttgtaattac tggtaagaat gaaagcctaa aagaaaaaat cgaaaaaaaa cttatgtctc
     1021 gttatcatga taaaaatgta tgtgttcttg gatacactga taagatgaat gatattttag
     1081 cttctataga tgtattagta agcaaacctg gtggacttac tacaactgag gctcttttaa
     1141 aagatgtacc tatgatagtt ccttattata taccaggtca ggaagaagag aatcttgatt
     1201 tcttatctaa ttgtggtgca gcacttagaa ctacaaaaaa atataacttg tcaatattac
     1261 taaaagtttt aatagatgac cctagcagat tagaaatgct aaaaagaaat attaaatcaa
     1321 taagaaagtc aaattcagcc caaaatatag ctaatttaat tttaaatata atttctggtt
     1381 gataaaataa ataaaatccc tatagtatat atattatcta ttagggattt tttattttct
     1441 aattatttaa tattaaattg gttcataaaa gtcttcttca taatcatact gataattttt
     1501 ataattattt ctaaattcag catttttatt tcgtacattt tttttagttt ttttcttatt
     1561 agaaaaactt atatcctcaa aatcatcata tttaggttta tcttttgatt tcattttttt
     1621 aactcttttt tctttcattg cattatgttc ttctaagttc ataattggtt tcatgttgca
     1681 cccctatcaa aatttttaaa tgatttatac aacaaattat acaatacaga agaaatttat
     1741 tcaataaatc taaatcaaaa ttaatttgaa tttaactcaa aaaattacaa aaatcgaggt
     1801 attaacctcg atttttatat ttctcttctg ccttctagtg cttttgatat tgtgacctca
     1861 tctgcatact ctaaatcgcc tccaacagga agaccatgag ctatccgagt gactttgatt
     1921 cccattggtt ttactaacct agcaatatac atggctgtag cttcaccttc aatagtagga
     1981 tttgttgcca ttattatttc tttgacttcc tgattgccaa gtctttggat aagttccttt
     2041 atttttaaca tatctggtcc aattccatcc attggagaaa ttactccatt taatacatgg
     2101 tactgtcctt taaactctct cgttctctcc atagctgcta catctcttgg atcttccaca
     2161 acacatataa cacttgaatc tctactttta tttgaacaca tgctacatgg gtctgtatct
     2221 gttatattac aacaaattga gcaatatctt atttctcttt ttgcatctat aatcgcttta
     2281 gataaagctt ccacatcatt agtattcata ttaataatat gaaaagccaa tctttgagca
     2341 gtctttcttc ctactccagg aagctttgaa aattcctcta taagtctcgt tatgggacct
     2401 gtataaactt gcataaatac cacctactag aataacccag gtatattcat acctccagtt
     2461 accttactca tttgacttgc ttgtatatca tcaatatttc tcaatgcttg atttactgca
     2521 cttaaaacta aatcttgtag catttctata tcatctggat caactacttc tggcttgata
     2581 gttatatcta taacttcttt tttaccatta acttttacag taactgctcc tccaccaact
     2641 gaagcttcta cttctttact ttctaactct tgttgagctt tttgcatatt ttcttgcatt
     2701 ttttgagcct gctttaacaa gttgttcata tttccaccac caggcatcat tccgcctcca
     2761 aaaccttttt tagccataaa cactattcct cctaaactta ttttcatttt attattatat
     2821 cataatagta ttttattatt tactctcatt ttcatcaata ctatctttta tttctaatat
     2881 ctcttcactc actatgttct ttaatatttc ctcaccaatg tcctttttct ctttaacttc
     2941 aagatttata tctttaactt ctgatgtcag tactattttt acattaaaac ttctatttaa
     3001 aacatctctt attgtttttt ctaaataatt tattgtatct tgagagctca gtctagtttt
     3061 tgcaaaatca aatttatcat caaatataac atacaacata ttagaatata cattaaaact
     3121 ttttacttca cttaagagtg cgtatattgg cattttttta tcttttttaa tttgtttaag
     3181 tatattcttc caagatgact ctataagtat aacgtcctca ttttttacat cttcataaac
     3241 tacattttct tgttttacat ctatttctct atcgacctca tactcttttt ctttattatt
     3301 cccaattttt tcagacttaa agttccccag ttcaatcatt ttttctaagt tctcaactct
     3361 ttttataaga gcttctttac tttcatcaaa cattggttga gcaattttca ttatggttat
     3421 ttccactaaa acccttggat ttgaagatga ctttatatca tcctgagtta ttgataatat
     3481 atttaaaatc cttattaaat catttatatc tatattttta gactgaattt taagttgttc
     3541 tatagtttct tctggtaatg aaattatttc atccaattct gatgaaacct tacataccat
     3601 taaatttcta aaatgatcaa ttaagtcatt tataagattt cttatatctt tgccccataa
     3661 gacaaattca tttaatatct caagagactt tttagtgtcc tgctctacta tacattgaga
     3721 caattcaaat aattgttcta tatttacgct tcccattaat tcaacaacat ctttataatc
     3781 tatcttactt tctccaaaag acatacattg gtctaatata ctaagtgcat ctctaagagc
     3841 tccttgagaa tttctagcta ttaaattaag agctttatca tctatatcaa ttccttcatc
     3901 ttcacaaatc tttttcattc taagggtcat atctttaact gtaactcttt taaaatcgta
     3961 tctttggcat cttgataaaa tggttgcagg tattttgtga ggctctgtag ttgctaatat
     4021 aaaaattaca tatgatggag gttcctctaa ggtctttaat aatgcattaa aagctccttg
     4081 agacaacata tgaacttcat ctattatata tactttatat ttagcattgg ctggagaata
     4141 tttcacactt tctctaagct ctcttatatc atctacacta ttatttgaag ctgcatctat
     4201 ttctacaaca tccattgtat tatcttttaa tacagattcg catatctcac actcattaca
     4261 cggttcttca tttattttat tcaaacagtt tactgctctt gaaaaaatct ttgcagtaga
     4321 tgttttacca gtacctcttg ttccacaaaa caaataagca tgtcctatat tatcactata
     4381 tatttgattc tttaaagtct taataatatg atcttgacca ataacatctt caaatttttg
     4441 tggtctatat gctctatata atgctttatg catcttttta cctctcttta gcaaaattaa
     4501 taaaaaaatc cctcattatc taagggatta gtcttttata tattaaatgc tgtacaccat
     4561 ctatcgactt aattccatag gcgttaccaa aacagttagc tcaacttagg cacccccacg
     4621 gcacacaaaa gtgatcactt accgctgctt ccttccggat ctgacggggt tcatgagctt
     4681 tcattgcgca ggacctaagc atcaacacca cttatttagg gcggccctac agaaaataag
     4741 cctttagatg gaattcaatc ctgctatagc ggattgcagg ttacagggca ccgctacctc
     4801 cccatctagt acagcaaaat tacaaccata tttaattact ttcattgttt ctggcggaga
     4861 gagtgggatt tgaacccacg atacagtttt acccatatac tcgctttcca ggcgagcgcc
     4921 ttcgaccact cgaccatctc tccttaatct ctttttctca atgccttaaa aaattcttgc
     4981 aatatgatcg agcattcttc ttgcaaaaca tcaaaagcta cttttacatt attattatta
     5041 aaataatcta atttaaactc atgctgtttt tctatatatg aattttttac atgtcttgtc
     5101 cctattataa gcttttttat tctagaatta actattgcgc cactacacat tatgcaaggt
     5161 tccattgtaa catataaatc acaatcagtc aacctccaac cacctaattt ttctgatgct
     5221 tgttttatcg ctaaaatttc tgcatgtgcc gtagagtctt ttaacgtttc tgtaaggtta
     5281 tgagctctag ctattatttg attatcttta actataatag cacctattgg tgtttctttt
     5341 ttattatatg ctttatatgc ttcttttaat gcttctttca tataaaatga actttccata
     5401 attcctccca aaacttataa caaacttatc ttatcataat tcattttata attcaactta
     5461 attttatttt gtttatttaa ttattactat atatagctat atttttttaa aattagttct
     5521 aaaataattt ttagatattc atacaagagt gttttataaa aatatttagt gttttatgat
     5581 gaaaattctg ctgtgtaaat taacataaaa aaactatgaa attaatattt ataataattt
     5641 catagttttt ttatttattg tttactcata atatattctc ttcatcaaag catactctat
     5701 atatacaatt aatataaatt tattttataa catcaacacc catataaggt cttaattctt
     5761 ctggaactac tacagaacca tctgcctgct gataattttc taatatagca gccaaacatc
     5821 ttcctattgc taatccagaa ccatttaatg tatgaacata ttctgctttt gattttttat
     5881 ctcttttaaa tcttattcct gctcttcttg cttggaaatc ttcaaaattt gaacaagaag
     5941 atatttctac atatctattg taacttggca tccaaacttc taaatcatat ttaaatgcag
     6001 ctgtaaatcc caaatctcct gtacatattt ttacaactct atatggaaga cctaacattt
     6061 gtaacatagt ttcagcatca tgagttaatg attctaattc attataagaa tcttctggtt
     6121 taacaaattt aactaattca actttattaa attgatgttg tctaactaaa cctcttgtat
     6181 ctcttcctgc tgaacctgct tcagatctaa aacaaggagt atatgcacag tatttaattg
     6241 gaagctctgc aacatctaat atttcattag catgtatatt agtaacagga acttcagctg
     6301 ttggtattaa gaaatactct aatccttcta ttttaaacat atcttcttca aatttaggaa
     6361 gttgaccagt ccctataaaa ctatttctat tggccataaa tggaggtaag acttccgtat
     6421 agccatgctt agctgtatga gtatttaaaa agaaattcat taaagatctt tctaatctag
     6481 ctcctaatcc cctatataaa gtaaaccttg acccagttat tttccctgct ctttcaaaat
     6541 ctaaaatacc tagacctgta cctatatccc aatgcgcttt atgctcaaaa tcaaatttag
     6601 taggttctcc ccaagttctt atttgaacat tgtcttcatc tgtttcacct tgtggtacct
     6661 ctggatgagg aacatttggt attctcatta atgtatattc catttttgct tctacttctt
     6721 ttactttctc atcaatacct tttatcttat ctgataactc tttcaatctt gccttttctt
     6781 ctgttacatc ttttccttct tttattaatt gaggtatttt tttagaattt acatttaact
     6841 catttttcat aacttcaact tcttgaagta tttctcttct tttattatct aattccacta
     6901 cagcatcaag gtcaaattct ctttcacctc ttctttccat agcttttttg atatcatcta
     6961 gattttctct tattctttta atatctaaca tattaattcc tcctaataat tttgataata
     7021 taagttataa ttccaactta atttacaaac attatacttg taaactctta gtttaaacaa
     7081 attttaaaaa taaaaaaaga cctccgttta ccctataaaa gggacgaaaa tcttttatcc
     7141 gcgttgccac cctaattgac tatactaaaa tgtataatcc tctcagacct ataacgtagg
     7201 taaacgtcta gctttaacta gaagctctag agtagattca aaaaccattg taatgagttt
     7261 tcaccatcca ctcattctct gaatacaatc tgttttttac tattttcctt cacagctttt
     7321 taactaattg ttttatttga taatcattat attatatata ttaacatctt tcaatactat
     7381 tattcaaatt tttttagaat tttctttaat tttttatatt aaattcattt tttctattta
     7441 attaaaacct tatcttttat ccattgtatt tagattacct gtattattaa ataataatga
     7501 agaaggaaca aatggaactt ttatatcatt ctcatcaaat ttatttttta ccttttccat
     7561 tacatcaaaa tatacatccc agtaatcatc agtttttacc caaggtctag caactatttg
     7621 catggtgtta tttcctatag agtttaaacc tattataggc tttggatcat ttaatattct
     7681 tttttcgttt gtaaaaattt catttaatat agacttaact acatctatat cactatcata
     7741 tgaaatatta tatgaaaaat ctattcttct tttgttttga tgtgtgtaat taattatatt
     7801 attacttgtc agttgaaaat taggtataat tatagtttta ttatctggcg tcttaagaac
     7861 agtgctaaat atttgtatgt cagaaactgt accttctaca cctgaacctt gaatataatc
     7921 acctatatta aatggcttaa aaaataaaat aatcatacca gagcctaaat ttgataatat
     7981 ttctttaaaa gctaaaccta tactaaatcc agcagctcct aacacagcta caactgatgt
     8041 agtttgtatc cctataatac ctaagcttat caatgatata atagataaca tagatatata
     8101 tattccatat acaataaaat tatttacacc tttactcatt ccatatttga ttagaaactt
     8161 tgaaaccatc cttcttacta ttttagatat ataaatacct atcacaaata caattattgc
     8221 atatataata tttggtatat tatcactaaa attatcgagc atattttcta aagttctctt
     8281 agttatattg ctatttgttg aggcagtctt agccaatgat aaaaaattca ttcaactcct
     8341 ccttatgata atattccgtt gtcctttaac tcttttacta tagcttctat agtttcttct
     8401 tttattgcct caattgtatg aatatggcta cattctgtca gaaccgataa ttgtttaaat
     8461 tcatttgtct caactttctt cataaagttg tctatatcca ttttagaata aatattaagt
     8521 tctgcagtta tctgaccata tgttggatga tttacgatga catcttttat aataccacct
     8581 aaatccacaa ttatctttaa ttcatcataa atttctttag aatccttatg atttttacac
     8641 tttatttgtt ttgtaaaata cggttgatta tatattatat atccttgagg tgtagctatt
     8701 atttcaaatc ctctagctct tataagggct atatcttgaa ctattacttg cctacttaca
     8761 tttaatagtt cagctagtgt tccacctttt acaggatgct tagattcttt taaaatatct
     8821 attaatttac ttcttctttc attagaattc atattacacc ttctttccaa ataaaaactt
     8881 tacctatact atataatttt atattttatc ttattataaa tcaatattga ctttatattc
     8941 tatttaatct taacttttat aataaataaa aaagac
//
LOCUS       DAMJYR010000140         8886 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.145, whole genome shotgun sequence.
ACCESSION   DAMJYR010000140 DAMJYR010000000
VERSION     DAMJYR010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8886)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8886)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8886
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.145"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(343..1239)
                     /locus_tag="PC471_003202"
     CDS             complement(343..1239)
                     /locus_tag="PC471_003202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HDF2790308.1"
                     /translation="MKDKNTYFNRKGQENFIDIFLKDDIKKGANFKRHWHEHLQIYYF
                     IDGIAYLECGLDNFKVVPGNIVIANSNELHYLESLSDNLKFYSIRIEPTFLFSNQVDL
                     LQTKYLAPLALNHIVFNNLIQNDSLILKCITTILDEYHNKEVGYELSIKANIYQLIVL
                     LLRSHIDKIYSEDEFIKRKYSLEKFECIFDLIEEKYMDKITLKELASHVNLSAHHFCR
                     TFKQITGKTTIDFINGIRLDKAIYYLKETDLNMTEIAMRCGFDSINYFSRLFKRNYNI
                     SPTKYRNEHKEVSNVILKEDMK"
     gene            1380..2807
                     /locus_tag="PC471_003203"
     CDS             1380..2807
                     /locus_tag="PC471_003203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDF2790309.1"
                     /translation="MTNSFKKDDNRKKITVLFVVIAMTFMATLDSSIINVALPVLASK
                     LNVSLASIEWVIASYSIIICSTLLFFGRLGDIIGKSRVFQVGTILFTSASLLCGLSNS
                     LTLLIVCRFIQGIGASAYMANNHGIITELFPKESRGKALGILVTAVAIGNMVGPSVGG
                     FILSIFDWNVIFFINIPIGLIVIFLNTKFLPNSKKSSENMDKTGAILQFLGTTLFFSA
                     LISAQQTGLLNPYILIALLLSIIFIILFLILEKKHPQPLLDLEIFRNFKFSLNLICAL
                     TSFICIASSSILIPFYLQSTMKLPPIQAGLFMILSPLILAIFSPIFGNISDKIKSEKI
                     ILIGLLVMSFGFFLMSRLKESSALILFVIYISIISIGQAIFQPANNALIMSSCSRSKL
                     GVVGSINSLVRNLGQVIGITISTTLLYNFMSIKAGYRVNDYVINNDKIFVFGMRNVYI
                     IVTLVCLIGAILIGFYLFKYNKNEQ"
     gene            complement(3003..5597)
                     /gene="clpB"
                     /locus_tag="PC471_003204"
     CDS             complement(3003..5597)
                     /gene="clpB"
                     /locus_tag="PC471_003204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HDF2790310.1"
                     /translation="MDVEKMTLRVQKSLNEAYNEAVKNHNQQVDVIHLFSALINQEDG
                     LIPNIIEKMNISIDSLRNTVNFEIDKLPKVYGEGADSQGVSATRKINEVLIKAESISK
                     EFKDSYISVEHVMLAMMETESKSAVGKILKQYNINKNNFLNILSQVRGSQRVETQDPE
                     GTYDALARYGTNLVDLAKKNKLDPVIGRDEEIRRIIRILSRRTKNNPVLIGEPGVGKT
                     AIVEGLAERIVRGDVPEGLKDKIVFSLDMGALIAGAKYRGEFEERLKAVLKEVQSSDG
                     KIILFIDEIHTIVGAGKTEGSMDAGNLIKPMLARGELNCIGATTFDEYRKYIEKDKAL
                     ERRFQPVIAEEPTVEDTISILRGLKERFEIHHGVRIHDNAIVAAAKLSHRYIQDRFLP
                     DKAIDLIDEAGAMIRSEIDSLPTELDVVRRKLFTLETEREALLKENDDKSKSRLDDIQ
                     KEIAELKSKNDEMTAKYEKEKSQILDIKNLKAQLDEAKGKAEKYEREYDFNKAAEVKY
                     GEIPKLEEQIKQYEENMNDGSENSLLKEEVTEEEISSIVSKWTGIPVTKLVEGEREKL
                     LKLEDELHKRVIGQDEAVTAVSNAVIRARAGLKDERKPIGSFIFLGPTGVGKTELAKT
                     LARNLFDSEDNIIRIDMSEYMEKHAVSRLVGPPPGYVGYEEGGQLTEAVRRAPYSVIL
                     FDEIEKAHEDVFNMFLQILDDGRLTDNKGKTVDFKNTLIIMTSNIGSNYLLEAGGNIT
                     ETTNNLVMNEMKHRFKPEFLNRVDDIIMFKPLDQENIKKIIDIFMKDLKNRLKEKDIT
                     IEVTNSAKDVMVREGYDPVYGARPLKRYIGNTLETIIAKKLIAGDVYNGCTIVIDGKD
                     ENIEVLVK"
     gene            complement(5634..6230)
                     /locus_tag="PC471_003205"
     CDS             complement(5634..6230)
                     /locus_tag="PC471_003205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2790311.1"
                     /translation="MSLKLDRNVLQWFDYVFENEKTSLRHYNFNCTLKEISSTSLNKV
                     AFILEKNNSKYWKLYFEIPAEVTLKLKQNIHPLFREYIYEQISLYNNNQIYNFVNSNI
                     LKVFNNIAIYQYNILENLYTIDFKKSFIDKCQYLLIGEKRLIDEDLYLIAKSKEVFDF
                     FNLDGTFNLTLSFDIQKNENLLDSLLELRKSIIINERI"
     gene            complement(6247..6651)
                     /locus_tag="PC471_003206"
     CDS             complement(6247..6651)
                     /locus_tag="PC471_003206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication/maintenance protein RepL"
                     /protein_id="HDF2790312.1"
                     /translation="MDKTLKKDLNQEYTESKSLIMNKSKYMNVCFKNYIKVVEGMYTK
                     RDLIPLKLINSMDKNNKIRKTLYELSCEFEYPKTSLSSLFTELKKMDFLQRVKNGEYM
                     INPHIAYKGSRADKESLLAEYNQIKRPNKVSK"
     gene            7018..7392
                     /locus_tag="PC471_003207"
     CDS             7018..7392
                     /locus_tag="PC471_003207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDF2790313.1"
                     /translation="MNIVISNSSGKPIYEQITTQIKSMIISGELPEGSALPSMRLLAK
                     ELRISVITTKRAYSDLERDGFIETVTGKGSFVSSKNMDFVKEEQFRLIEEYLQKAVDT
                     AKSSDITYDELKDILFLLYKGE"
     gene            7389..8252
                     /locus_tag="PC471_003208"
     CDS             7389..8252
                     /locus_tag="PC471_003208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2790314.1"
                     /translation="MISMDAITIKNLNKTYKDFSLQDISFSVPKGSVMGFVGENGAGK
                     TTTLKAILNLISYDSGNIEIFGLDSKKNEKEIKEQIGVVFEGSNFHENLNTDHVSKIM
                     SKIYKNWNDTLFKDYLKKLRVPDNKLIKEFSKGNKMKLSIAVALSHKPKLLILDEATS
                     SLDPIVREEILDIFLDFIQDEEHSIILSSHITSDLDKIADYITFIHKGKIVFSENKDE
                     LIDTMGVLKCKPSDFDNLSREDYSYYRKSQFGYEVLLKDKHRFISRHPNCIVDNTSIE
                     EIMLFYVRGDK"
     gene            8252..>8886
                     /locus_tag="PC471_003209"
     CDS             8252..>8886
                     /locus_tag="PC471_003209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 transporter permease"
                     /protein_id="HDF2790315.1"
                     /translation="MKGLILKDLLNLKGNIKFILLFIIMFGFMSSLGDGNVNNFIGVI
                     IVLCTTMIVSTFSYDDLNKWDSYVLTMPINRNDIVLSKYLTMLIFSFIGVLVSLIVSV
                     TIGYFKNTLILNETLLINALILSISVCFGSLILPLIYKFGTERARLLMILCFLVPTLA
                     LLVFKSILENISSPISIEIILNTLVYSLPFVAILLFVISYFISSKIYSKKEV"
BASE COUNT         2976 a         1310 c         1024 g         3576 t
ORIGIN      
        1 ctttaggatg gatttaaatt ttctataaaa atcatactat tttagctgtt tacttgctta
       61 tttaatagtt ttatttcttt gattaaatat ctttcagaat ctaagccaga tgtctcaaaa
      121 aaactgattt ataatataat cattttttct gctattctta tttttccaat tttagtttca
      181 tattaaaata tacttttcat agacactaca ttctttcata ctcattttca catttaacta
      241 tacaagtagt cttactcatt tatgtactat actttattaa tatttattaa gaaaaactca
      301 aagatttaaa aatatatatt ttctataaag tatatttatg aattatttca tatcttcttt
      361 taaaataaca ttactaactt ctttatgttc atttctatac ttagttggag atatattata
      421 attcctttta aataagcgac taaaataatt tatgctatca aagccacatc tcatagcaat
      481 ttctgtcata tttaaatctg tttcctttaa ataatatata gctttatcaa gcctgattcc
      541 atttataaaa tcgattgtag tcttacctgt tatttgctta aatgttctac aaaagtgatg
      601 tgcactcaag ttcacatgac tagcaagttc ttttaatgta attttatcca tatatttttc
      661 ttctatcaaa tcaaagatac attcaaattt ttctaaagaa tactttcttt ttataaattc
      721 atcttcagaa tatattttat caatatgact tcttaataag agtactatta attgatagat
      781 attagctttt atagataact catatccaac ttctttattg tgatattcat caagtatagt
      841 tgtaatacat tttaaaatca gagaatcatt ttgaattaaa ttattaaata caatatgatt
      901 taaagccaat ggtgctaagt acttggtttg taacaaatca acttgattac taaaaaggaa
      961 tgtgggttca atacgaatac tatagaattt taaattatct gataagcttt ctaaataatg
     1021 taattcatta ctgtttgcta ttacaatatt tccaggcact accttaaaat tatccaatcc
     1081 acattcaaga tatgctatac catcaataaa ataatatatt tgtagatgct catgccaatg
     1141 tcttttaaag tttgctcctt ttttaatgtc atcctttaaa aaaatatcta taaagttttc
     1201 ttgacctttc ctgttaaaat atgtattttt atccttcatt attcccccct catcaaaata
     1261 gtgctaatta taagcaatat agttattgta ttcattattt catatcgcta ttatataaaa
     1321 tgtaactatc attgtaaaca ttaacataat gcagtcatta taataaggag gtaataaaaa
     1381 tgacaaactc ttttaaaaaa gatgataata gaaaaaaaat cacagtttta tttgtagtca
     1441 ttgccatgac ttttatggca acattagatt ctagtattat aaatgttgct ctcccagttc
     1501 ttgcaagcaa actaaatgta tctctagcat ctattgaatg ggtaatagca agttactcaa
     1561 ttattatttg ttcaacacta ttattttttg gaagacttgg agatattatt ggtaaatcaa
     1621 gagtatttca agtaggtact attctcttta catcagcttc attactatgt ggattaagta
     1681 attctttaac tttacttata gtttgccgct ttattcaagg aataggcgct tctgcctaca
     1741 tggcaaacaa tcatggaatt atcacagaac tatttccaaa agaaagcaga ggaaaagccc
     1801 tagggatttt agttactgca gttgctattg gaaatatggt tggaccatct gtgggaggat
     1861 ttattttgtc aatatttgat tggaatgtta tattttttat aaatattcca atcggtctta
     1921 ttgtaatctt tttaaataca aaatttcttc ctaatagtaa aaaatcttca gaaaatatgg
     1981 ataaaactgg tgctatactt caatttttag gaacaacatt attttttagt gctctaatta
     2041 gtgctcaaca aacaggatta ctaaatccat atatattaat tgcactatta ttatcaatta
     2101 tttttattat attatttttg atattagaaa aaaaacatcc tcaaccgctt cttgaccttg
     2161 aaatttttag aaattttaaa ttttcattga atttaatttg tgctcttaca tcattcattt
     2221 gtattgcatc atcaagtatt ttaattccat tttatttaca aagtactatg aaacttcccc
     2281 ctattcaagc aggtttattt atgatactat cacctttgat tttagccatt ttttcaccta
     2341 tatttgggaa tatatcagat aaaattaagt cagaaaaaat tattttaatt ggtcttctcg
     2401 ttatgagttt tggattcttt ctaatgtcaa gattgaaaga aagttctgcc cttattttat
     2461 ttgtaattta tatatcgata atatcaatag gacaagctat atttcaacct gctaacaatg
     2521 ctttgataat gtcaagctgt tctagaagta agctaggagt tgtaggaagc ataaattccc
     2581 ttgtaagaaa tttaggacaa gttataggta ttactatttc tacaacactt ttatataatt
     2641 ttatgagtat taaagctgga tatagggtca atgattatgt gattaataat gataaaatat
     2701 ttgtatttgg aatgagaaat gtttatatta ttgttacact tgtttgctta ataggtgcaa
     2761 tattaattgg cttttattta tttaaatata ataaaaatga acaatagttt ttataattat
     2821 attcaaatta tgattatatt ataagaaatt tactaaaata cttcctttga gttaaattta
     2881 atttggacat attgacttaa aaatgtttta ttaatttgat aaaatatttc ttttcctata
     2941 gtttataaat aaaaatcagc tgagcaaaat atttagtata ttatgctcaa ctgattcttt
     3001 tattatttaa ctaaaacttc aatattttca tcttttccat ctataactat agtacatcca
     3061 ttatacacat ctccagcaat aagtttcttt gcaattatag tttctaatgt atttcctata
     3121 tatctcttaa gaggtcttgc tccatataca gggtcatatc cttctctcac cataacatct
     3181 tttgctgaat ttgtaacttc aatagtaata tctttttctt ttaatctatt ttttaaatct
     3241 ttcataaata tatctataat tttcttgata ttttcttggt ctaaaggctt aaacattata
     3301 atatcatcta ctctgtttaa aaactcaggt ttaaatctat gcttcatttc attcattact
     3361 aaattgttag tagtctctgt tatatttcct cctgcttcta atagataatt acttcctata
     3421 ttagaagtca ttattataag agtattttta aagtctactg tcttaccttt attatcagta
     3481 agtctaccat catctaatat ctgtaagaac atgttaaata catcctcatg tgctttttct
     3541 atctcatcaa atagtattac agaataagga gcacgtctaa ctgcttctgt aagttgacca
     3601 ccctcttcat atcccacgta ccctggagga ggtccaacta gtcttgatac agcatgtttt
     3661 tccatgtatt cagacatatc tattcttatt atattatctt cactatcgaa taaatttcta
     3721 gcaagagttt ttgcaagttc agttttacca acacctgtag ggcctaagaa aataaatgaa
     3781 ccaattggtt ttctttcatc ttttaatcct gctcttgcac gtattactgc atttgaaact
     3841 gctgtaactg cttcatcttg tccaataact cttttatgaa gttcatcttc tagtttaagt
     3901 aatttttctc tttctccttc aactagttta gttactggta tacctgtcca ctttgataca
     3961 atgctagata tttcttcttc agtaacttct tcttttaaaa gagaattttc agaaccatca
     4021 ttcatatttt cttcgtactg ctttatttgt tcttctaact taggtatttc accatattta
     4081 acctctgctg ctttgttgaa atcatattct ctttcatact tttcagcttt acctttagct
     4141 tcgtctaatt gagcctttaa gtttttaata tctaatattt gactcttttc tttttcatat
     4201 tttgctgtca tttcatcatt tttagatttt aactctgcta tttctttttg aatatcatcc
     4261 aatcttgatt tacttttatc atcattttct ttaagtaatg cttctctttc agtttctaat
     4321 gtaaataact ttcttctaac aacatctaat tctgttggca atgaatctat ttcactacga
     4381 atcatagctc cagcttcatc aattaaatca attgctttat ctggtaaaaa tctatcttgt
     4441 atatatctat gagaaagttt tgctgctgct acaatagcat tatcatgtat tcttactcca
     4501 tgatgtatct caaatctttc ttttaaacct ctaagtatag atattgtatc ttcaacagta
     4561 ggttcttctg caattacagg ctgaaaacgt ctttcaagag ccttatcttt ttctatatac
     4621 tttctatatt catcaaaagt agttgcacca atacagttca attcacctct tgcaagcata
     4681 ggtttaataa gattacctgc atccattgaa ccttctgttt taccagctcc aacaatagta
     4741 tgaatctcat ctataaacag tataattttt ccatcagaac tttgaacttc ttttaaaact
     4801 gcctttaatc tttcttcaaa ttcacctcta tattttgcac cagcaattaa agctcccata
     4861 tcaagtgaga atacaatctt atcttttaaa ccttctggga catctcctct tactattctc
     4921 tctgctaacc cttctacaat tgcagtttta cctacacctg gctcaccaat caatacagga
     4981 ttattttttg ttcttcttga taaaattctt ataatccttc ttatttcttc atctctccca
     5041 attacagggt ctaatttatt tttcttggct aaatctacta aatttgttcc atatctagca
     5101 agtgcatcat atgtgccttc tgggtcttgt gtttctactc tttgactacc tctcacttgt
     5161 gataaaatat ttaaaaagtt attcttattt atattatatt gttttaatat tttaccaaca
     5221 gctgattttg attctgtttc catcattgca agcataacat gctctacact tatatatgaa
     5281 tctttaaatt ctttagaaat actctctgcc tttataagca cctcatttat ttttctagta
     5341 gctgatactc cttgtgagtc agcaccttct ccatatacct ttggaagctt atcaatttca
     5401 aagtttacag tatttctcaa agaatcaatt gatatattca ttttttcgat tatatttggt
     5461 atcaatccat cctcttgatt aataagtgct gagaaaagat gaattacatc tacttgttgg
     5521 ttatgatttt ttactgcttc gttataagcc tcatttaaac ttttttgaac tcttaaagtc
     5581 attttttcta cgtccatata aatccctcca ttttaatatt atatccaaat aaattaaatt
     5641 ctctcattta taattattga ttttctaagc tctaaaagac tatcaagtaa attctcgttt
     5701 ttttgtatat cgaaactcaa tgttaaatta aatgttccat ctaaattaaa aaaatcgaat
     5761 acttcttttg attttgctat taaatacaag tcttcatcta taagtctttt ttcacctatt
     5821 aataaatact gacatttatc tataaaactc tttttaaaat caatagtgta tagattttct
     5881 aaaatattat attgataaat agcaatatta ttaaacactt ttaatatgtt tgaatttaca
     5941 aaattgtaaa tttgattatt gttatacagt gatatttgtt cgtatatata ttccctaaat
     6001 aaaggatgaa tgttttgctt aagttttaaa gtaacttcag ctggtatttc aaaataaagc
     6061 ttccagtact tagagttatt tttttctaaa ataaaagcta ctttgttcaa actggtagaa
     6121 ctaatctcct ttagtgtaca attaaaatta tagtgtctca aactagtttt ttcattctca
     6181 aatacatagt caaaccactg tagaacattt ctatcaagtt ttaaactcat ataaaaacct
     6241 tcttccttat ttactaacct tattaggtct ttttatctga ttatattctg ctaataaact
     6301 ttctttatca gctctactac ctttatatgc tatatgtgga tttatcatat actcaccatt
     6361 tttcactctt tgtaaaaaat ccattttttt cagctccgta aacaatgaag ataaagaagt
     6421 ttttggatat tcaaattcac aagataattc atataatgtt tttctaatct tattattttt
     6481 atccatagag tttattaatt ttaatggaat taaatccctc ttagtataca ttccttctac
     6541 tactttaata taattcttaa aacaaacatt catatactta cttttattca taattaaact
     6601 tttgctctct gtatattcct gatttaaatc cttttttaat gtcttatcca aattaaatat
     6661 ctcttctcac tctccttatc tctaattgat aatataaata tattacaaaa aaatatgtat
     6721 gtcaaaactg aaaatgcaaa aagttcacct tataagtaaa cttttattta gctataagct
     6781 aaactttatt atttttttaa tttagtattt ccaatttatt ggcaggattt ttgaacaata
     6841 tttataaatt ttttaaaaaa atttaaatct atttaatatg gcagactttc ataaaataca
     6901 ttacatttat taaatgtata atattggtaa ttagtttttt ctaaaatcag ttgacatatt
     6961 atatatatat ttatataatg tgtatatact atatatacaa tttaaggagg tgtcttattg
     7021 aatatagtta ttagtaattc gagtgggaag ccaatatatg aacagattac tacacaaatt
     7081 aaaagtatga ttataagtgg agagttacca gaaggctcag ctcttccaag tatgagacta
     7141 ttggcaaagg aacttagaat aagtgtaatt actacaaaac gagcatatag cgatttagaa
     7201 cgagacggat ttattgagac agttactggt aaaggcagtt ttgtatctag taaaaacatg
     7261 gattttgtca aagaagaaca atttcgttta attgaagaat acctacaaaa agcagttgat
     7321 acagcaaaaa gtagtgatat tacatatgac gaattaaaag atattttatt tttattatat
     7381 aaaggagaat gataagtatg gatgcaataa ctataaaaaa tttaaataaa acttataaag
     7441 attttagcct acaagatata tctttttcag taccaaaagg gagtgtaatg ggatttgtag
     7501 gtgaaaatgg tgcaggaaaa acaactacat taaaagcaat tttaaattta atatcttatg
     7561 atagtggaaa tatagaaatc tttggtcttg atagtaaaaa aaatgaaaaa gaaattaaag
     7621 agcaaattgg ggttgtattt gagggaagca actttcatga aaatttaaac actgaccatg
     7681 tttcaaaaat catgagtaaa atctataaaa attggaatga cacattattt aaagactact
     7741 taaaaaaatt gagagtacca gacaataaat taattaagga gttttcaaag gggaataaga
     7801 tgaaacttag tattgctgta gccttatctc ataaaccaaa attattaata ctagatgagg
     7861 caactagttc acttgaccct atcgttcgtg aagaaatact agatatattt ttagatttta
     7921 tacaagatga agaacactct attatcttat cttcacatat tacaagtgat ttagataaga
     7981 tagcagacta tattacattt atacataaag gaaaaattgt atttagtgaa aataaggatg
     8041 aattaattga tactatggga gttttaaaat gtaaacctag tgattttgat aacctttcaa
     8101 gagaggatta ttcatattat cgtaaaagcc aatttggata tgaagtactt cttaaagata
     8161 agcatagatt tatctcaaga catccaaact gtattgttga taatacgtct atagaagaaa
     8221 ttatgttatt ttatgtgaga ggtgataaat aatgaaaggt ctaattttaa aagatttact
     8281 taatttgaaa ggaaatataa agtttatact tttattcatc attatgtttg gctttatgtc
     8341 ttctttagga gatggaaatg ttaataactt tataggtgtt attatagttt tatgtactac
     8401 aatgattgtt tcaacatttt catatgatga tttgaataaa tgggatagtt atgtacttac
     8461 aatgcctatt aatcgcaatg atattgtatt aagtaaatat ttaactatgt taatatttag
     8521 ctttataggt gttcttgttt cacttattgt tagtgtaact attggttatt ttaaaaatac
     8581 acttatatta aatgaaacac tacttattaa tgcactaatc ttatcaattt cagtatgttt
     8641 tggtagcctt attctaccac ttatttataa atttggtaca gaaagagctc gtttattaat
     8701 gattttatgc tttttagttc caacactagc tttattagtt tttaaaagta ttttagaaaa
     8761 tattagttca cctatttcta tagaaattat acttaataca ctagtatatt ctcttccatt
     8821 tgttgctatt ttactatttg tgatttcata ttttatatca tcaaaaatct atagtaaaaa
     8881 agaagt
//
LOCUS       DAMJYR010000141         8780 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.146, whole genome shotgun sequence.
ACCESSION   DAMJYR010000141 DAMJYR010000000
VERSION     DAMJYR010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8780)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8780)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8780
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.146"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            66..833
                     /locus_tag="PC471_003210"
     CDS             66..833
                     /locus_tag="PC471_003210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaP domain-containing protein"
                     /protein_id="HDF2790316.1"
                     /translation="MQIIDININNIDNEHICCSISEKKGENCVFSKKAWMKNNFDKGL
                     VFKRFDVRGKAFIEYIPAENAWCPITADNYIFINCFWISGKFKGQGYSNLLLQECVDD
                     AKSKGKYGLVVLSSSKKMPFLSDAKYLKYKDFCLADTSEPNYELLYFPFTDNAPIPYF
                     KNCVKKRKLNENGIVLYYSNQCPHTEKYAPILKKIANSRGIDVNLIKFETAKEAQNAP
                     APFTTYSLFFNGEFVTNEILSEKKIEKFLDTNIKKFN"
     misc_binding    1051..1264
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1518..2552
                     /locus_tag="PC471_003211"
     CDS             1518..2552
                     /locus_tag="PC471_003211"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HDF2790317.1"
                     /translation="MNKIKVGIIGATGYVGAELIRLLMNHDKVEVTAIGSNSYVGKDI
                     VDIYPSIGYKNNMICIENEKVIDMCDVVFTALPHGVSEKFVIKAIKSKKKVIDLGADF
                     RIKDEEVYSKWYGVSFIDKILHKKAVYGLSEIYKEDIKDADIIANPGCYPTSISLPLM
                     PLLSSKLIKNNNIIIDSKSGLTGAGRELSISSHFTEVNENITAYKIGKHRHTPEIEQN
                     LSESCKEKVSVVFTPNLIPVNRGILSTIYCTKEDNISINDIHRKLTDYYEFKEFIEIL
                     PLDKVASLKNVRFSNKCVISLHENGDTLIICSAIDNMIKGAAGQAIQNMNIIFGIEEN
                     TGLKNIAPSF"
     gene            2593..3810
                     /gene="argJ"
                     /locus_tag="PC471_003212"
     CDS             2593..3810
                     /gene="argJ"
                     /locus_tag="PC471_003212"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HDF2790318.1"
                     /translation="MEIIKGGVTVSEGFFASGIHCGLRKNKEKRDLALVYSDVLCDAA
                     AVVTQNKVKGNPVYVTQEHIKNGKAQAIIVNSANANTFNGKEGLINAYKMAKFTSDKL
                     NIKESDVLVASTGVIGKPLNIDLIEENMDELVNNLSKQGHIGAREAIMTTDIIKKEIA
                     VAIMYGDKKITIGGMAKGSGMIHPNMATTLGFITTDANIDGVLLKEALKIAVDKSFNR
                     VSVDGDTSTNDMVMILANGKAKNDRINKKDEHYQVFLSALTYVCIELAKLVAKDGEGC
                     TKLIECYINGALSEEHAVKLAKTVISSSLVKTAVFGADANWGRILCALGYAGEEIDME
                     KVDIIFESMKGYIEVCKNGNGLDFNEEKAKKILEDDEISILVDLNMGNARGNAWGCDL
                     SYDYVRINGSYRN"
     gene            3825..4685
                     /gene="argB"
                     /locus_tag="PC471_003213"
     CDS             3825..4685
                     /gene="argB"
                     /locus_tag="PC471_003213"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HDF2790319.1"
                     /translation="MINIEKANTLIEALPYIEKHQGKTIVVKYGGSAMKKDGLKESVM
                     EDLVLMSYVGINIVLVHGGGAEINKMLAKVDIESKFVNGLRYTDEETMEIVKMVLAGK
                     VNKDLVNKIHTKGGKAVGLCGIDNNMILCDPYKNYELGFVGEIKKVNVELIESCLKSG
                     YISVIATIGVGDDGETYNINGDTAASAIAKELNADKLILLTDVPGLLREPDEEKSLIT
                     EVILEDVDKLFEEGIITGGMIPKIEGCVDALNNGVNRVHILDGRVPHSIITELFTDSG
                     IGTLIRKETE"
     gene            4715..5902
                     /locus_tag="PC471_003214"
     CDS             4715..5902
                     /locus_tag="PC471_003214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HDF2790320.1"
                     /translation="MNNDNTISKWNEYFIDTYNQPNFVIDYGEGSCFFDTNGNKYIDF
                     TSGYGVSSLGYSNSNLKNALKEQVDKLLHTSNLYFNEPVLYSGEKIINSSGMAKVYFC
                     NSGTEANETAFKIARKYSSDKYGNGRGTIISLKDSFHGRTMMSLMATGMDKYHKYFYP
                     LPEGFKYIERNNIEDLKNNLDSTVCAIILEAIQGEGGVNVLEKDYVLEIVKICQEKDI
                     VVIFDEVQCGIGRTGKLFGYEYFDVKPDIVTVAKGLGAGIPVGGVLVNKKLSKVLGKG
                     DQGTTFGGNLLAMVAASVVLDEISKDGFYNEVLEKGNYIRKSIESFNNKVILKTKGIG
                     LMIGIETNIESSIIEEKARKKGLLILTAGKNVLRFLPPLTISYKEIDEALEILKDILL
                     EIN"
     gene            6011..7033
                     /gene="argF"
                     /locus_tag="PC471_003215"
     CDS             6011..7033
                     /gene="argF"
                     /locus_tag="PC471_003215"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HDF2790321.1"
                     /translation="MNNISLKGKSFLTLKDFTKEEIRYLLTLSSYLKTKKKVGIKGDL
                     LQGKNIALLFEKTSTRTRCSFEVAAHDEGAHVTYLGPNDCHMGKKESVSDTAKVLGRF
                     YDGIEFRGFKQETVESLAKYSGVPVWNGLTDEYHPTQILADFLTVIENVDKDLNKVKF
                     VYVGDARNNMGNSLMIGCAKMGIHFVALAPKELWPNEELVKEMKEIAKKSKGDIMLTE
                     NIDDVKGADVIYTDVWVSMGEEEQYEARINQLKDYQVNMDMIKKTENENVIFLHCLPA
                     FHDLETKVAQEIYEKFGLSELEVTNEVFNSKYSKVFEEAENRMHTIKAVMVSTMTDVN
                     MNNNLI"
     gene            7160..7777
                     /locus_tag="PC471_003216"
     CDS             7160..7777
                     /locus_tag="PC471_003216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4878 domain-containing protein"
                     /protein_id="HDF2790322.1"
                     /translation="MKKIILALTTFLLAISLTACSSAKPEDTIDSFFNSAKKFDFEGM
                     NKVMENNDEKYKDILKELDTKDPNAQYVLDYLKQNASKITYTIKDSEVKGDKATIKVE
                     CKFIDSTPLLQEIVAEAFTKMIGMSFSGQDLTDEKTTEMLVSIMKEKQKSVKETYVTK
                     NVEFECSKKDNKWIISSVNDAVADVLLSNLVTAGQEFSNSMNTLE"
     gene            complement(7997..8695)
                     /locus_tag="PC471_003217"
     CDS             complement(7997..8695)
                     /locus_tag="PC471_003217"
                     /EC_number="5.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HDF2790323.1"
                     /translation="MNEKLIGILAGMGPRSTAPFVDLIIDECQSQYGAKYDDEFPKMM
                     IYSLPTPFYIDRPINHDLMKETIIDGLQKLESTGVNFIAMPCNSAHIYFKELKESINI
                     PILNIVEETVKKLPTISQKVTLFSTSSTFESTIYQNGIIHNGHEFIFKDEWQIKLNNL
                     IQNIKMDKENPHNIDIWNKLIEDVKNESIENIVIACTDLNVVLEKSYPSINIVDSSKC
                     LAESVVNKYLKLVK"
BASE COUNT         3534 a          855 c         1577 g         2814 t
ORIGIN      
        1 atattaagat aaaaatatat agaatatatt taaaaattat tatgcaatta gaaaaggaga
       61 taagtatgca gattattgat ataaatataa ataatattga caatgaacat atatgttgtt
      121 caatttctga aaaaaaagga gaaaattgtg ttttttctaa aaaagcttgg atgaaaaata
      181 attttgataa aggtctagtc tttaaacgat ttgatgtaag aggtaaagct tttatagaat
      241 atattccagc agaaaatgca tggtgtccta ttactgcgga caattatata tttattaact
      301 gtttttggat atctggaaaa tttaagggtc aaggatattc taatctattg ctacaagagt
      361 gtgttgatga tgcaaaatca aaaggtaaat atggattagt tgtgctttcg tctagtaaaa
      421 aaatgccttt tttatctgat gctaaatatc taaaatataa ggacttttgt ttagcagata
      481 catcagaacc aaattatgag ctattatatt ttccttttac tgataatgct cctattccat
      541 attttaagaa ttgtgtaaaa aaaagaaaat taaatgagaa cggtatagtt ttatattaca
      601 gtaatcagtg tccacatacg gaaaaatatg ctcctatttt gaaaaaaatt gcaaattcta
      661 gaggtattga tgtaaatctt ataaaattcg aaacagcaaa agaagcacaa aatgctcctg
      721 caccatttac tacatacagt ctttttttta atggtgaatt tgtaaccaac gagatacttt
      781 ctgagaaaaa gattgagaaa tttttagata ccaacattaa aaaatttaac taaaaagaga
      841 tgtaaatatt gtattttgaa aatatattat tttataattt aatatatttt ggatgttttt
      901 taaattgacg aatgaaatat aaaaattata tcgcaattta gatacaaaat gctggaaatt
      961 aaccatatag aaaatatttc caaaaattta aaaattatat tgacaaagtt aaaaaaataa
     1021 actatcatat aaaacaataa gaaaataaat tgaaatcctg tgaaaaagag agtagcataa
     1081 agctagttga tagagagctg ggaatggtgg aagcctagta acgaatttta ttgtgaatag
     1141 agctttggag gagattacca gaagtaatag tatggtaatt ggtgaatctc gccttaagag
     1201 attagagtgg gtaagattat cttatcaatt agagtggtaa cgcgggtgta tattcgtctc
     1261 ttggtctttg accaagaggc ttttttaatt ttaaatgtac attttgtaga tactttttaa
     1321 tttgagtttt aatgattatt gtaattttac caataagagt ggtaccgcgg aaaatcgcct
     1381 cttagtattt ataatgctaa ggggtttttt gttttaatta gagtatttta atctacgtat
     1441 tatcaaatga ttatcatata gtaaagtgga aagataggat tttaagacta tttgaatata
     1501 aaaatggagg gaacaaaatg aataaaataa aagttggtat tattggtgca actggatatg
     1561 tcggggcaga gttgattaga ttattaatga atcatgataa agtcgaagtg acagcgatag
     1621 gttcaaattc gtatgttgga aaagatatag ttgatatata tccaagtata ggatataaaa
     1681 ataacatgat atgtatagaa aatgaaaaag ttatagatat gtgtgatgtt gtatttacag
     1741 cccttccaca tggagttagt gaaaagtttg taataaaggc aattaaatcg aaaaagaaag
     1801 ttatagattt aggtgctgac tttagaataa aagatgaaga agtatatagt aaatggtatg
     1861 gagtatcttt tatagataaa atcttacata aaaaagctgt atatggatta agtgaaatat
     1921 acaaagaaga tataaaagat gcagatataa ttgcaaatcc tggatgttat ccaactagta
     1981 tttctttacc attgatgcca ttactaagtt ctaaattgat aaaaaataat aatataataa
     2041 ttgattctaa atcaggtctt acaggtgctg gtagagaatt atcgattagt agtcacttta
     2101 cggaggttaa tgaaaatatc actgcatata aaatagggaa gcacagacat actccagaaa
     2161 tagaacaaaa tttaagtgaa tcctgcaaag aaaaagtaag tgtagttttt acaccaaact
     2221 taatcccagt aaatagggga atcttatcaa ctatatattg tacaaaagaa gataatattt
     2281 ctataaatga tatacatagg aaattaactg attattatga atttaaagaa tttatagaaa
     2341 ttcttccatt agataaggta gcttctttaa agaatgttag attctcaaat aaatgtgtaa
     2401 tatcattaca tgaaaatgga gatacattga taatctgttc agctatagat aatatgatta
     2461 agggagcagc aggtcaagct atacaaaaca tgaatataat atttggtatt gaagaaaata
     2521 caggtttaaa aaatattgca ccatcatttt agaaataaaa aatactaaac tcatataatt
     2581 aggaggataa atatggagat tataaagggt ggagttacag tttcagaagg attttttgct
     2641 tcagggattc actgtggctt gagaaaaaat aaagaaaaaa gagatttagc tttggtttat
     2701 tcagatgttt tatgtgatgc agcagcagta gtaactcaaa ataaagtaaa gggcaatcct
     2761 gtttatgtta cacaggaaca cattaaaaat ggaaaagcac aagctatcat agtaaatagt
     2821 gctaatgcta atacctttaa tggaaaagaa ggacttataa atgcttataa aatggctaaa
     2881 ttcaccagtg ataaattaaa tattaaagaa agtgatgttt tggttgcatc tacaggtgtt
     2941 ataggaaagc cattaaatat agatttaata gaagaaaata tggatgaatt agtgaataat
     3001 ctaagtaaac aaggtcatat tggagcaaga gaagctatta tgacaactga tattataaaa
     3061 aaagaaatag cagtagcaat aatgtatgga gataaaaaaa taactattgg aggtatggca
     3121 aaaggttctg gcatgataca tcctaatatg gcaacaacgt taggatttat aactacagat
     3181 gcaaatattg atggagtttt gctaaaagaa gctttaaaaa ttgctgttga caaaagtttt
     3241 aatagagtta gtgtagatgg agatacaagt actaatgata tggtaatgat tctagcaaat
     3301 ggaaaagcaa aaaatgatag aataaacaaa aaagatgaac attatcaagt atttttgagt
     3361 gcacttacat atgtctgtat agaacttgct aaacttgttg caaaagatgg agaaggatgt
     3421 acaaaactaa ttgaatgcta tataaatgga gcattaagtg aagaacatgc agtaaaatta
     3481 gcaaaaacag ttatatcttc ttcattagta aagacagctg tatttggagc tgacgcaaat
     3541 tggggtagaa tactgtgtgc tttaggttat gcaggtgaag aaatagatat ggaaaaagtt
     3601 gatattattt ttgagagtat gaaaggatat attgaagtat gtaaaaatgg aaatggatta
     3661 gattttaatg aggaaaaagc aaaaaaaatc cttgaagatg acgaaataag tatattagta
     3721 gatttaaata tgggcaatgc cagaggaaat gcatggggat gtgatttaag ctatgattat
     3781 gtaagaataa atggaagtta tagaaattag gaggatatac tttaatgatt aatatagaaa
     3841 aagcaaatac acttatagaa gctttaccat atatagaaaa acatcaggga aagactattg
     3901 tagttaagta tggtggaagt gcaatgaaaa aagatggtct aaaagaatct gttatggaag
     3961 accttgtgtt aatgagttat gttggaatca atatagtttt agtacatggt ggaggggcag
     4021 aaatcaataa gatgctagca aaagtggata tagaaagtaa atttgtaaat ggtcttagat
     4081 atacagatga agagaccatg gaaattgtaa aaatggtact tgctggaaag gttaataaag
     4141 acttagtaaa taaaatacat acaaaaggtg gaaaagcagt agggctttgt ggaatagata
     4201 ataatatgat tttatgtgac ccatataaaa attatgaact tggatttgta ggagaaatca
     4261 aaaaagtaaa tgttgagctt atagaatctt gtctaaagtc aggttatatt tcagtaattg
     4321 caactatagg tgttggagat gatggagaga cttataatat aaatggtgac acagcagctt
     4381 ctgctatagc taaagagtta aatgcagata aattgatact tcttacagat gtccctggtt
     4441 tgttaagaga gccagatgaa gaaaaatcat taataacaga agttatatta gaggatgttg
     4501 ataaattatt tgaagaaggt ataattactg gaggtatgat acctaaaata gagggatgcg
     4561 tagatgcctt aaataacgga gtaaataggg tacatatttt ggatggaaga gttccacatt
     4621 ctataataac agagctattt actgatagtg gaataggtac attaataaga aaagaaactg
     4681 aataaaatta taagtttaag aggggagaat aagaatgaat aacgacaata caatttcaaa
     4741 atggaatgaa tattttatag atacatataa ccaacccaat tttgtcatag attatggtga
     4801 aggaagttgt ttttttgata ctaatggcaa taaatatata gattttacaa gtggatatgg
     4861 agtatcttct cttgggtata gtaatagtaa tttaaagaat gctctaaaag agcaagtaga
     4921 taaattatta catacttcaa atttatattt taatgagcca gtgttgtatt caggagagaa
     4981 aataattaat tcaagtggaa tggctaaagt atatttttgt aattctggaa cagaagcaaa
     5041 tgaaacagca ttcaaaatag caagaaagta tagtagtgac aaatatggta atggtagagg
     5101 aactatcata agtttaaaag actcttttca tggtagaaca atgatgtctt taatggcaac
     5161 tggtatggac aaataccata agtattttta tcctcttccc gaaggtttta aatatataga
     5221 aagaaataat attgaagatt taaaaaataa tcttgattcc acagtttgtg caataattct
     5281 tgaagcgatt caaggcgaag gtggagttaa tgttcttgaa aaagattatg ttttggaaat
     5341 agtaaaaata tgtcaagaaa aggatattgt agtgattttt gatgaagtcc aatgcggtat
     5401 aggaagaact ggtaaacttt ttggctatga atattttgat gtaaaaccag atatagttac
     5461 cgtagcaaaa gggttgggag caggaatacc agtaggagga gtattggtaa ataaaaaact
     5521 ttcaaaagta ctaggaaaag gagaccaagg tacaactttt ggaggtaatt tattagctat
     5581 ggtggcagct tcagttgtat tagatgagat atctaaagat ggcttttata atgaagtatt
     5641 agaaaaagga aattatataa gaaaatctat tgaaagtttt aataacaaag ttatattaaa
     5701 gacaaaaggt attggtctaa tgataggaat tgaaactaat attgaatcta gtattataga
     5761 agagaaagct agaaaaaaag gattgcttat tttgactgct ggaaaaaatg tattgagatt
     5821 tttaccacca ttaacaatca gttataagga aatagatgag gctcttgaaa tcctaaaaga
     5881 tatattacta gaaattaact aaatattcaa aaaatagttg attaaagata aaaattagaa
     5941 gtaaataaaa aagtaaagtg ctaacaagtt taattgaatt taaaaagttt ataagaaggg
     6001 gcgaattaat atgaataata taagtttaaa aggtaagagt tttttgacat taaaggattt
     6061 tacaaaagaa gagattagat atttattaac tttatcaagt tatttaaaaa ctaagaaaaa
     6121 agttggtata aaaggcgatt tgctacaagg aaaaaatata gctttattat ttgagaaaac
     6181 atcaacaaga actagatgtt catttgaagt agcagctcat gatgaaggag cacatgtaac
     6241 ttatctaggt ccaaatgatt gtcatatggg taaaaaagag tctgtatcag atacagcaaa
     6301 agttctagga agattctatg atggaataga atttagaggc tttaaacaag aaacagttga
     6361 aagtctagca aaatattctg gtgtacctgt ttggaatgga cttacagatg aatatcatcc
     6421 aacacaaata ctggcagatt ttttaacagt aattgaaaat gtagataaag atttgaataa
     6481 agttaaattt gtatatgttg gtgatgctag aaataatatg gggaactcac tgatgatagg
     6541 atgtgctaaa atgggaatac atttcgtagc actagcacct aaggaacttt ggccaaatga
     6601 agaattggtt aaggaaatga aggaaatagc caagaaatcc aaaggagata taatgttgac
     6661 tgaaaatatt gatgatgtta aaggtgcaga tgttatatat acagatgtat gggtttctat
     6721 gggagaagag gagcaatatg aagcaagaat aaatcaactt aaagattatc aagtaaatat
     6781 ggatatgata aagaaaactg aaaatgaaaa tgttatattt ttacactgtc ttccagcatt
     6841 tcatgattta gaaactaagg ttgctcagga gatttatgaa aagttcggat tatcagaatt
     6901 agaggttaca aacgaggtat ttaattcaaa atattcaaag gtatttgaag aagctgaaaa
     6961 tagaatgcat acaataaaag ctgttatggt ttctactatg actgatgtaa atatgaacaa
     7021 taatttaata tagatatgtt ttaatataga ctttacttta atgaaaattt ataaaatatt
     7081 ttaaatatat tattcaaaaa ctctcattat atgttaaaat aacttatgta tcaacaaatt
     7141 aagagggagg atttgagata tgaaaaaaat catattagct ttaacaactt ttttattagc
     7201 aatatcttta acagcatgtt caagtgcaaa accagaagat acaattgata gtttttttaa
     7261 cagtgcgaaa aagtttgact ttgaaggcat gaataaggta atggaaaata atgacgaaaa
     7321 atataaggat atattaaagg aattagatac aaaagaccca aatgcccaat atgttttaga
     7381 ttatctaaaa cagaatgcat ctaaaatcac atatacaata aaagacagcg aggttaaagg
     7441 tgataaggct acaatcaaag tagaatgtaa atttatagat tcaacaccat tacttcaaga
     7501 aatagtagct gaggcattta caaagatgat tggtatgagt ttttcaggac aagacttaac
     7561 tgatgagaaa acaactgaaa tgttagtatc aattatgaaa gaaaaacaaa aaagcgttaa
     7621 agagacttat gttacaaaaa atgtagagtt tgaatgttct aaaaaggata ataaatggat
     7681 aataagttca gtaaatgatg cagtagctga tgtattgtta tctaatcttg ttacagcagg
     7741 acaagaattt tctaactcta tgaatacact tgaataatat tgtttaggtg tagtaaagta
     7801 ataaatatat gattattttt ataaataaac atataaatat ttatgaagga taccattttt
     7861 ggtatccttt ttattaatat aatgaaatac atggttaatt tctatattgt caagaaacta
     7921 tgatatatgt tataagtcta tactatttaa aataaactta taacatatat tttttataat
     7981 atagtgtatg aaaaatttat ttaactaatt ttagatattt attgacaact gattcagcca
     8041 agcattttga tgaatcaaca atatttattg aaggatatga tttttctaaa actacattta
     8101 aatcagtaca agcaattact atattttcta tagattcatt tttaacatct tcaataagtt
     8161 tattccaaat atcaatattg tgtggatttt ctttatccat ttttatattt tgaataagat
     8221 tgtttaattt tatttgccat tcatctttaa atataaattc atgtccatta tgtattatac
     8281 cattctggta aattgtagac tcaaaagttg aactagtcga aaaaagagtt actttttgtg
     8341 agatagtagg taatttttta actgtttctt ctacaatatt taatattggt atattaatag
     8401 actcttttaa ttctttaaag tatatatgtg ctgaattgca tggcattgca ataaaattta
     8461 caccagttga ttcaagcttt tgtaatccat caattatagt ttctttcata aggtcatgat
     8521 ttattggacg gtcaatataa aatggggtag gtaatgaata tatcatcatc tttggaaatt
     8581 catcatcata ttttgctcca tattgagatt gacactcatc aataattaaa tcaacaaaag
     8641 gggctgttga ccttggaccc attccagcca aaataccaat taatttttca ttcataaaaa
     8701 ttatctcctt tatatataaa tatatttatg tataaaataa aaatctataa aagcataaaa
     8761 ttatatttta taatagatta
//
LOCUS       DAMJYR010000142         8716 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.147, whole genome shotgun sequence.
ACCESSION   DAMJYR010000142 DAMJYR010000000
VERSION     DAMJYR010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8716)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8716)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8716
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.147"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(533..1285)
                     /locus_tag="PC471_003218"
     CDS             complement(533..1285)
                     /locus_tag="PC471_003218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HDF2790324.1"
                     /translation="MFSVIFLCIGGFLAAFVDSIAGGGGLISMPVLMAIGVPVHLAIG
                     TNKFAASAGCISSAYRYAKSGKINNNLLKKLVPFTIIGSVLGVRCVLSISEEILNVLV
                     VVMILIVAIYTFISKNLGQEDNFEAVNKKNLRLGMLMALIMGFYDGFFGPGTGTFLTF
                     GFIKIYGYDFLHASANTKILNLTSNITSLLLFMINGQVDYKIAIVFALVMIMGAYVGA
                     KVAIKKGSKMIKPIFLVMALFMVVKLVYQTLV"
     gene            complement(1344..2999)
                     /locus_tag="PC471_003219"
     CDS             complement(1344..2999)
                     /locus_tag="PC471_003219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF2790325.1"
                     /translation="MEYFSESNNEVQKFDWGEVMWIHEPSKTQFNRLSAGIVRFFPGN
                     YQEKHFHLSEEQLLYVIQGEGIQIIDGKKVNIKETSIVYCPPYSEHEIINTGKIDLVI
                     LITYVPHKFSSLRQIPIVFSENNIQELVNVNIIENLANQISNILKLRISVYDLKYKEI
                     FETKKENKFCDICKNIKQCTKKLVKSINNDNFEKVYHCEYGVSSLEIPIVLEENIVGY
                     IECGNFIVYKSIDIDKNLSELSSSSGIDIKYINKIYNEFPLNPKSRLYVLKENLLMMS
                     EFIQEIAKRNFFENQLSIKDEEILKSRKENIRLEEALKKANSRLLEEEYIINPIDMFN
                     NSSKEYPFELELYIEKEIKNMNLEGVYNLIDTNKLRYNDVRDTIQEMIFVLSRTVLRD
                     LEDLKLISYLRNKYNKKISLVNSDEDLWNVLFEFSKECIDKNRAFWRKDKGNLIENIN
                     EYIKKYYKENINLNSISDVFFISPNYLSSIFNERNKVSITEYINLLRIEESKKYLLDR
                     SMSISDICKKVGFNNSSYFSQIFKKFNSITPNEYRKNMLDNKY"
     gene            complement(4272..5351)
                     /locus_tag="PC471_003220"
     CDS             complement(4272..5351)
                     /locus_tag="PC471_003220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDF2790326.1"
                     /translation="MILITLCILVAVFVLAFAGLRLIAPTIIRTSHKIEAPGIDRMEM
                     VEIGGIQQALYIRGQNADNPIILWVHGGPGNSMMPFLHLYQYEWENDFTVVNWDQRNV
                     GKTYFANDPAAVLQTMSAERVLQDVHEVTAYLKQEFNKEKIILIGHSWGTVLGTMAVQ
                     TYPEDYSAYIGIAQTVNMVDNERVGYEKTLEMVKAAGKQKDIKALEAIAPYPPAKYGP
                     SYLAALMTVRTYQSKYGLSINMDLGNIISILATPYYSLRELKNTAIFNPNAIDNQGDV
                     LRFLVEEADIRNYGVDYQVPVYYIMGENDYNSPYLPAKKFFDEIVAPDKQIFFIPDAD
                     HLPFLSKKAEFDCVLLEEIAPKLAK"
     gene            complement(5435..6091)
                     /locus_tag="PC471_003221"
     CDS             complement(5435..6091)
                     /locus_tag="PC471_003221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDF2790327.1"
                     /translation="MTRKNNPKQAIENIITISAKLFAEKGYDKTSMQNIVDASGMSKG
                     GIFHHFSSKEDIFNVVMERRFEQIIETVNQWLGEMHGLTAKEKLRSLVRRHLTDEVIK
                     ESSNMITSAIESPHIILAFTQDNLKKLAPILANVLREGIEDRSIITEFPDECAEVILL
                     LFNFWCDTDVFQGDFPTLRKRFQFLQFLMRQIGVDILEDEIIESINIFYERKPMGQVE
                     "
     gene            6413..6619
                     /locus_tag="PC471_003222"
     CDS             6413..6619
                     /locus_tag="PC471_003222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2790328.1"
                     /translation="MISHAAEICKKYKMPFIIFPTIEKKSIEEWVNIGANMLCLAQDT
                     TFITYVINQLLSFYNEIVPIQKQH"
     gene            complement(6772..7200)
                     /locus_tag="PC471_003223"
     CDS             complement(6772..7200)
                     /locus_tag="PC471_003223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HDF2790329.1"
                     /translation="MSKIYIVYWSGTGNTEKMANFVAEGVKLKGKTPEVLDVSLLKPS
                     DLKEEDKFALGCPSMGAEQLEEGYMEPFVSELESMVSGKQIGLFGSYGWGNCEWMRDW
                     EERMQNAGATIIGGEGITAMEDPNEEAKDECIELGKTLAE"
     gene            complement(7676..8614)
                     /locus_tag="PC471_003224"
     CDS             complement(7676..8614)
                     /locus_tag="PC471_003224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HDF2790330.1"
                     /translation="MNKRMKLIPYEINENLRGAKNKFPYGIKQMNARGMWDEGYTGKN
                     IVVGIIDTGCDISHPLLKGKIIGGANFSDDSNGNKNIYEDFNGHGTHVAGIIAASNYN
                     NEVMGVAPDCKLLIAKALNKDGTGTYQSIINAINFAVNNKVDIISMSLGGNKDDKNLK
                     NAVMQAVKNNISVVCAAGNNGDGDSSTSEYSYPASYAEVIEVGAINENYLVEKFSNSN
                     TTIDLVAPGRNIISTYMDNKLAIMSGTSMSAPYVSGSLALIKEWAREEFERDLDEAEL
                     YAQLIKCTRALGIPRTEQGNGYLYLNLYKYKNNSKR"
BASE COUNT         2806 a         1601 c          947 g         3362 t
ORIGIN      
        1 aaattaattt agcataacct tgttaaatat agatttgaat atgaatttaa agttatgtaa
       61 aattaaaaat acaggaatga aaattccatt cctgtataaa atctaacttt tgagtctcag
      121 tacaatctta ttttgagaca atcttttttg ttttatattt tcttatattt aaaatataac
      181 gcatatctta tgcaacatat tcatattttt ctttatattc tttcatcgca tcattccaag
      241 cgtcttgtac tgttacacct cttttattag caattttttg tgccagttca attatcttca
      301 tagctcttct tgatatgttc ataaacattc ctcctacaaa ttaccatata tttcatatat
      361 ataaatcaag gttatatttt tatgatatat aatccttcta ttgtaagatt acagtttata
      421 tgtgtattat atatgaacta tattagaaga taattagaat ttttataaag ctatatgatt
      481 agattaaata taaaagcttt acaattactt ataaatttaa aaagtctata agctaaacta
      541 atgtttgata taccaatttt acgaccataa ataatgccat tactaaaaat attggtttta
      601 tcatctttga acctttcttt atagcaactt tagcacctac ataagctccc attatcatca
      661 ctaatgcaaa cactatagca attttataat ctacttgacc atttatcatg aataataaca
      721 atgaagttat attacttgtt aagtttaata tttttgtatt tgcagaagca tgtaaaaaat
      781 catatccata tatttttata aatccaaaag tcaaaaaagt tcctgtacct ggaccaaaaa
      841 aaccatcata aaaacccatt ataagagcca taagcatccc taatcttaaa tttttcttat
      901 taacagcttc aaaattatct tcttgaccta aatttttaga aataaaggtg taaatagcca
      961 ctattaaaat cataaccaca accaaaacat ttaatatctc ttcacttatt gataatacac
     1021 atctcacacc tagaacacta ccaataatag taaatggtac taattttttt aaaaggttgt
     1081 tattaatttt tccagacttt gcatatctat aggcactact tatacatcct gctgatgctg
     1141 caaatttatt tgtaccaatg gcaagatgta ctggcactcc aattgccata agaacaggca
     1201 tgcttataag tcctcctcct cctgctatag aatctacaaa agcagctaaa aaccccccta
     1261 tacataaaaa aataactgaa aacataaaat tcctccatca ttctctttca atttttctta
     1321 caatatatat tatataatct tatttaatac ttattatcca gcatattctt tctatattca
     1381 tttggagtta tactattaaa cttcttaaat atttgtgaaa aataactact gttattaaaa
     1441 cctactttct tacaaatatc actaatactc atgcttctat ctaacagata ttttttagat
     1501 tcttctatcc taagaagatt tatatactca gttattgaaa ctttatttct ttcattaaaa
     1561 atagaactca aataatttgg acttataaaa aaaacatctg atatactatt caaatttata
     1621 ttttctttat aatatttttt tatatattcg tttatatttt caattagatt tcctttatct
     1681 tttctccaaa atgctctatt cttatcaata cactctttgg aaaattcaaa taatacattc
     1741 cataaatctt catcactgtt tacaagtgat atttttttat tatatttatt tcttaaataa
     1801 gatataagtt ttaaatcctc taaatctcta agcactgttc tagacaatac aaatatcatt
     1861 tcctgaatag tatctctaac atcattatat cttaatttgt ttgtatctat tagattataa
     1921 acaccttcta aattcatatt tttaatttct ttttcaatat aaagttctaa ttcaaaggga
     1981 tattcttttg aagaattatt gaacatatct atcggattaa taatatactc ttcttctaat
     2041 aatctactat ttgctttttt aagtgcctct tcaagtctta tattttcttt tctactcttc
     2101 aatatctcct catcttttat actaagttga ttttcaaaaa aattcctctt agctatttcc
     2161 tgaataaatt cagacatcat taataaattt tcttttaata catataatct acttttagga
     2221 tttaatggaa attcattgta aattttgttt atgtatttta tatcaatacc agaactactt
     2281 gataattcag ataaattttt atctatatca atagacttat atactataaa attaccacat
     2341 tctatatatc caactatatt ttcttctaat actataggta tctctaaact ggaaacacca
     2401 tactcacaat ggtacacttt ttcaaagtta tcattattta tgcttttaac aagtttttta
     2461 gtacactgtt ttatattttt acaaatatcg caaaatttat tttctttctt agtctcaaat
     2521 atttctttat actttaagtc atatacagat attctaagct tcaatatatt agatatttga
     2581 tttgcaagat tttcaattat gtttacatta actaactcct gtatattgtt ttcagaaaat
     2641 actataggta tttgtctcaa actcgaaaat ttatgaggaa cataagtaat taatattact
     2701 aaatcaattt taccagtgtt tataatttca tgttctgaat atggtgggca ataaactata
     2761 gatgtttcct ttatattgac ctttttacca tcaattattt gtattccttc tccctgaatc
     2821 acatataaaa gctgttcttc acttaaatga aaatgctttt cttggtagtt tcccggaaaa
     2881 aatcttacaa ttccagcact aagtctattg aattgtgttt ttgatggttc gtgtatccac
     2941 ataacctctc cccaatcaaa tttttgtact tcattatttg actcactaaa atactccata
     3001 agctcacctc ttagtttttg tctaaatatt ttttcttccc ttcttatatt aaaataatat
     3061 catcatcatt aactttcatt actataaaat catcatactc ttttaatact cctgtaattt
     3121 tagttgcact tagtttacta caattcagtt ttttaatata ttgataaatt ctataattac
     3181 ttttttattt tgtaaaatca gtgttttccc aaaataatta tatctataaa tgaaagtatt
     3241 tttactgctt acatcaatat atgttatcat aattttcctt tttattgcat atataaataa
     3301 ctgtatataa cattgtccaa tactatatag aaatacatct aagaaagaat attattaaat
     3361 tgttccaatt agtattattt ttcttataga aataaaaaaa ctgttggagt cttttagaaa
     3421 aattattttt tacatacctt ttttgcttag atagtctatc ttcacagcta ttttgtatat
     3481 acactcttct agttcatgat tttattttaa tatcttgctt atattgctga aataattgaa
     3541 ttcttttatc tatcatattg tctcttatat cacaaacata ttgtttctta tatcacaaaa
     3601 atgaaagcat tcttatatct tcaatatata aacctgcttt ttttaatctt gtaataaata
     3661 taggatactc tatattctct tcctagaaat aactaatacc acttattttt ctattatata
     3721 gaaagtaata aatctttttc ttaataaaat tcaatagtaa gtggaataaa attttatatg
     3781 aataagaaca ttataaaaat actttataca atagtcaata aaagatatta gcttcaaatt
     3841 atgttaaagt tgtattattt aaagtgaaaa aacaaataaa aaagaaataa tagtataagt
     3901 agaaaaatat acttaaaaca aggcgaagcc tctcttgtat ctaaatcaag tagagaatta
     3961 aatctaaaaa agaaaataaa tataaaagat gactcctgta cattacaaga atcatcttat
     4021 ggtttcataa tcactccatg gtttttaact gtctgatata aaggtctcag ttcactcata
     4081 gcatgaccac cttttccgat tgctgacccc gtcacttatg gacaaaatgt ccataagtga
     4141 tagtcctctc ttaaaaccct ctaaatagat atatctcaag caccgcattg aaaccagttg
     4201 ctggaaacaa actgaagcgt tctggaatag taaaagaaat tgccaccata tccacgttac
     4261 tgtcctagtt gttactttgc taacttcgga gcgatttcct ctaacaatac gcagtcaaat
     4321 tctgcctttt tgctaagaaa tggcagatgg tctgcatctg gtataaaaaa aatctgtttg
     4381 tctggcgcca ctatttcatc aaaaaatttc ttcgctggaa gataggggct attatagtca
     4441 ttttctccca tgatgtagta tacagggacc tgatagtcaa ctccataatt tcttatgtca
     4501 gcttcttcca ctaaaaaccg caatacatca ccttgattgt caatcgcatt tggattgaaa
     4561 atggccgtat tttttaactc tctcaatgaa taatacgggg ttgccagtat actgattata
     4621 ttgcccaaat ccatatttat agaaagccca tacttgcttt ggtatgttct tactgtcata
     4681 agagctgcca aatagcttgg accatatttt gctggcggat atggtgcaat agcttccagc
     4741 gccttgatat ctttttgctt ccctgcagct ttcaccattt caagtgtctt ttcatagccc
     4801 acgcgctcat tgtccaccat attaacggtc tgcgcaatcc caatgtacgc gctgtaatcc
     4861 tccggatagg tttgcacagc catagttccc agaactgttc cccagctatg acctataagg
     4921 ataattttct ccttgttgaa ctcttgcttc aaatatgctg tcacctcatg gacgtcttgc
     4981 agaacacgct ccgcgctcat ggtctgcaaa actgctgccg gatcgttcgc aaagtatgtt
     5041 ttgcctacgt ttcgctgatc ccagttcacc acagtaaaat cattttccca ctcatactgg
     5101 tacagatgta aaaatggcat cattgaatta ccaggacccc catgaaccca aaggatgatg
     5161 gggttgtctg cgttttgtcc ccggatatat aacgcctgct gtatgccgcc gatctccacc
     5221 atttccatgc ggtcgattcc aggagcttct attttatggc ttgtacgtat aatcgtgggg
     5281 gctatcagac gcaaaccggc aaacgccaga acaaaaacag caactagtat gcaaagtgtg
     5341 attaatatca ttttaagtac cttctttcat gaattttttt agccatagat atctcctcta
     5401 acattaaaaa aaatatgttg ctattttagt tttgttactc tacctgtccc attggtttcc
     5461 tttcataaaa aatattaatt gattctataa tctcatcttc caggatatca actcctattt
     5521 gtctcataag aaactgtaag aattgaaacc gtttacgaag tgtaggaaag tccccctgaa
     5581 atacatctgt atcacaccaa aagttaaaga gcagcaaaat tacctctgcg cattcatctg
     5641 ggaactctgt gatgatgctt ctatcctcta tgccttctcg taaaacattt gctaatattg
     5701 gtgctagttt tttcaaatta tcctgtgtaa atgccagtat gatatgcgga ctttctatcg
     5761 cgcttgtaat catgttacta gattctttta tcacctcatc cgttaaatgc cgcctaacta
     5821 aacttctaag cttctctttt gctgtaagtc catgcatttc accgagccat tgatttactg
     5881 tctctattat ctgttcaaag cgcctttcca tgaccacatt aaaaatatct tcttttgaac
     5941 tgaagtgatg aaaaattccg ccctttgaca ttcctgaagc atcaacaata ttttgcatac
     6001 ttgttttgtc atatcctttt tcagcaaaca actttgcaga aatggtaatg atattttcaa
     6061 tagcttgctt tggattgttc tttctagtca ttatataact ccttcttatt aaaaaccgac
     6121 tgttggtttt tattatataa gctattcctg cttctgtcaa gtaacattgt gtttatggca
     6181 aaacaataag gtttggtggt tagacaggta ttaattagac taatgcgact ttcccctaga
     6241 tggaaccctt ggtaaatttg caatctttac cggctttgat tatatctgta agcaaggcag
     6301 gtatggccac acctgccctg tttgcccctc ggatattcat tcctatccct cctctcctta
     6361 gccattacac caaaacgccg ctcattgtct ccaatggcgg cgttttaaag atatgatttc
     6421 tcatgctgct gaaatatgca aaaaatataa gatgcccttt attatttttc ctacaataga
     6481 aaagaaaagt attgaagaat gggttaatat tggagcaaat atgctttgtt tggcacaaga
     6541 tactaccttt atcacttatg ttatcaatca attattatct ttttataatg agattgtacc
     6601 aattcaaaaa caacattaaa ttataaatct aattttatat taaaagagac taatatttta
     6661 tctatctcta ttaaggatat tttcttatgt atcataatat tagtctctct tattattata
     6721 tactgctatc atcattagtt gctatttata ctatttttat aatatacaaa tttattcagc
     6781 taacgttttg cctaattcta tacactcatc ttttgcttct tcatttgggt cttccattgc
     6841 tgtaattcct tctccaccaa taattgtagc accagcattt tgcatacgtt cttcccagtc
     6901 tctcatccat tcacaatttc cccatccata tgaaccaaat aatccaatct gtttacctga
     6961 taccatagat tctaattctg aaacaaatgg ctccatatac ccctcttcta gttgctctgc
     7021 tcccatagat gggcaaccta atgcaaattt atcttcttct tttaaatcac ttggtttcag
     7081 taagctcaca tctaaaactt ctggtgtctt accttttaac ttcacacctt cagccacaaa
     7141 atttgccatt ttttcagtat ttcctgttcc actccaataa acaatatata ttttactcat
     7201 ttttacctcc tgtagtattt tttaattaat caatagttag ttatagctaa ctaacatcta
     7261 aaaaacaaaa gatttcttta tctgaaatct ctttattttt ctaaatatag aatatcatgg
     7321 ttgattttga aattcaatac ttattgataa tatttatcat ttatatttta tattcatttt
     7381 ataatatcaa aacactgtat cgtatttcta atctttcata gaaaaatttc taacttatag
     7441 tacttttgca tctcttataa atcttttata tgtataagta catagaaaaa cacacctttt
     7501 ccagatgtgc tatctataat atcgtatatt tatttttcaa ttctaactag ctaatatgtt
     7561 tataaatttt atcacttaat acctttatac attgatgtaa aatcttacac ctaagtaatt
     7621 tttattattt gtctcgactc tatataatat agctatttta tcatcaaatc aaaaattatc
     7681 ttttgctatt attcttgtat ttataaagat ttaaatataa gtatccattt ccttgttccg
     7741 ttctaggtat tccaagcgct ctcgtacatt ttattaattg tgcatacagt tcagcttcat
     7801 ctaaatctct ttcaaactcc tctcttgccc attctttaat tagtgctaat gagcctgata
     7861 cgtatggtgc actcatactg gtaccactca taatagcaag tttattatcc atataagtcg
     7921 atataatatt tcttcctgga gctaccaaat ctattgtagt atttgaatta ctaaactttt
     7981 caaccaaata gttctcattt attgcaccta cttctattac ctcagcataa ctggctggat
     8041 aactatactc acttgtacta gaatcaccat ctccattatt acctgctgca cacactacag
     8101 aaatattatt ttttactgct tgcatgacag cattttttaa attcttatca tctttgtttc
     8161 ccccaagaga catagatata atatcaacct tgttatttac agcaaagtta atagcattaa
     8221 ttatactttg atatgttcca gtaccatctt tatttaatgc ttttgctatt aataatttac
     8281 aatctggagc tactcccata acttcattat tataattaga tgcagctata atacccgcca
     8341 catgagttcc atgaccatta aaatcctcat atatattttt atttccatta ctgtcatcac
     8401 taaaatttgc accaccaatt atttttcctt ttaaaagagg atgagatata tcacaacctg
     8461 tatctattat accaactaca atatttttac cagtataacc ttcatcccac attcccctag
     8521 cattcatttg ttttattcca tatgggaatt tattttttgc acctcttaga ttttcattta
     8581 tttcatatgg aattagtttc attcttttat tcatcatata tcctcctaaa aattttattg
     8641 cttataaaaa tcatgaattt tattttataa taggtttata aatcaaatta aaacctatat
     8701 ctacttattt atattt
//
LOCUS       DAMJYR010000143         8697 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.148, whole genome shotgun sequence.
ACCESSION   DAMJYR010000143 DAMJYR010000000
VERSION     DAMJYR010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8697)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8697)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8697
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.148"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            135..1916
                     /locus_tag="PC471_003225"
     CDS             135..1916
                     /locus_tag="PC471_003225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HDF2790331.1"
                     /translation="MSQYININSQLDALKLKEFEKIFYENINEDVKVVPKVTPFKGIN
                     TDLLYVKDGRILFIKFMDTTEDIFFILEEELLEVMNEEYELLKLKMGQKNRNISYNYV
                     YIMPYVEVEETYEFEEFVNNNIIDKNKLQDIMNKGSLEEYLNDENDEINLNLFLLDIC
                     SEYYIINDKLHLNEKFKKISFYNDDYKYTATMMEDVQIKDVVSIKYGNTLIEGGSGIG
                     KTAIMLSRAIKLAKVYPHHKLVIFTHTKQLCNELRERIELLYKDNNNLEVHTFSSFIF
                     KLAKKFNLIVDYNMLKNDYEKTFNNLVKQAQNIIKNKNMFKAIFIDEAESFLEYEIDF
                     IREFLYKTKFIFNVCSCNSLNISNRLNIFKKLYNGIEFDDKIILSKNYRQAKEIVDFT
                     NKFSNNSNSYINELRPNTEFSTFFYTKALRGGNKSVDIIKVSDLDDQISSVIWEIEYL
                     ISKKGLDYSEVAIVYPYNKKKLKSGKTIYFQYMLKKALEEAKIPYICAEDNLTNISKK
                     VGTTIANIYAIKNLEYKAVIVCELEMLYNQTINDIEQDYQVNDFVGDLNKVYLAMSRA
                     TDYLSIVTTFNEEASDIIRLITESKDI"
     gene            complement(1998..2528)
                     /gene="lepB"
                     /locus_tag="PC471_003226"
     CDS             complement(1998..2528)
                     /gene="lepB"
                     /locus_tag="PC471_003226"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HDF2790332.1"
                     /translation="MSVKKEIFDWIKSIAMAIVLAFVILQFIRPSIVSGESMYPTLDD
                     KDYLILNRISYKVGKPEKGDIVVFKTNLVDGETGKKKDLIKRVIATEGDRIKISNSKV
                     YVNGKLLNEPYIHNNYTSGDIDTVVPKGKLFAMGDNRENSNDSRFPDVGMVDEDEVLG
                     KVMVRLLPLDNIGKVD"
     gene            complement(2568..3392)
                     /locus_tag="PC471_003227"
     CDS             complement(2568..3392)
                     /locus_tag="PC471_003227"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HDF2790333.1"
                     /translation="METFSLLEIFKAVILGIVQGITEWLPVSSTGHMILVDEFIKLNF
                     SNTFISTFLVVIQFGSILAVLVIFFRKLNPFDSAKNIKQKKETVRLWLKVIIAVIPSG
                     VIGILFEDDIDRLFFNSTVVAIALIVYGIIMIGLEKRNKKPKYKDFSQVTYKLALCIG
                     LFQCLALIPGTSRSGSTIIGAVLLGTSRYVAAEFSFFLAIPTMLGASALKLLKAGFGF
                     TGFEWLILGVGSVVAFVVSIVVIKFFMDYIKKHDFKVFGYYRIVLGIVVLAYFFLL"
     gene            3649..4557
                     /locus_tag="PC471_003228"
     CDS             3649..4557
                     /locus_tag="PC471_003228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDF2790334.1"
                     /translation="MYSTKILDDYEYETFNVFSTKLNPNTKHDYLSKVILFKEFLKGK
                     ELIYATKEDCKNFVDYIQTKYAKSTCEKIYSYLHSFYNFLKKEGYIDINPFRYVEKPT
                     VTRIKTKDDVLSIQEINKLIGILPKLNIRDRVIIVCLVTTGCLLNELVSLKWKDLMVD
                     ENDNSYVRLGKGRKERVVKLHPYFFKLLEDYRNYSGLPEVIIPSDDFIFTTQKSNSIT
                     DRNVRLIVEKALDLAGLSQYSARDFRHSFAAISLRLGADESDVKNQLGWSDKYYAIRY
                     KYVLNFVDSEIVDYLIEKDNLDINKK"
     gene            4701..6146
                     /locus_tag="PC471_003229"
     CDS             4701..6146
                     /locus_tag="PC471_003229"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HDF2790335.1"
                     /translation="MIDNKYTTYSNPLTDRYCSKDMSYIFSPQFKFSTWRRLWVALAE
                     GEHELGINITEEQLDELRANIDNINFDEARKIEKEVRHDVMSHVKAYGLQCEKAKGII
                     HLGATSAYVGDNTDVIQMNEALKLLRVKLVNLINNLKEFALKYKDMPALGFTHFQAAQ
                     LTTVGKRACLWAQDLILDLEDLNYRIDNMKLRGVKGTTGTQASFLSLFEGDHEKVKEL
                     DKKICKKMGYHSSYAVSGQTYTRKLDYQVISILSGIAQSMHKMTNDIRLLQSLKELEE
                     PFEKNQIGSSAMAYKRNPMRSERIASLSKYIISESISPAMVQATQWLERTLDDSANKR
                     LAIPQAFMAADAILEIGINVTDGLVVYENMINRRVNEELPFMATETILMEAVKRGGDR
                     QELHEIIREYSMKAAYRVKHEGKDNNLIELIMNDDSFKMSKEEILSIMDPKNFIGRAP
                     EQVVEFVNEVVEPAIKDYKEDLGKVDVDLRV"
     gene            6457..6909
                     /locus_tag="PC471_003230"
     CDS             6457..6909
                     /locus_tag="PC471_003230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDF2790336.1"
                     /translation="MNYIVKDTISFLVKTFPKIYSSLYLEDLKKFAPDYNVNKTQLRA
                     LVFIKNYGVISMTDLCSKLNIEKGSLTSMVDDLTDKKYVTRKRDLVDRRKYLIDITEE
                     GDKIATDFMDKLSDGLGEKLSKLTEEDRKKYLEAINTLQYILNKEEFR"
     gene            complement(7244..8440)
                     /locus_tag="PC471_003231"
     CDS             complement(7244..8440)
                     /locus_tag="PC471_003231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HDF2790337.1"
                     /translation="MNYSNRVSAMQASPIRKLVPFAQAAKDKGIKVYHLNIGQPDIKT
                     PKGFFDAVKNFDSEVLEYATSEGIPELLEALQNYYKTYDMNFEKDELLVTNGGSEALL
                     FTMMAVCDPGDNLLVPEPFYTNYNGFGQSVNVEVNAVTTKAENGFHLPSKEEILSKVD
                     DKTKAIILSNPGNPTGAIYTKEELNILAEIAKEKDLWIIADEVYREFVYDGLEYTSCG
                     NLEGVQDRVIIIDSVSKRYSACGARIGSIACKNKGLIAQILKLCQGRLCVPTLEQIGA
                     VELYKTPVSYFKEVNEEYKKRRDVLYNELMKVEGVICKKPTGAFYIVAKLPVENAEDF
                     TIWMLKEFNKDNETVMVCPAEGFYATPGLGRDEIRLAYILNEKDLHRAATLLKEGLEQ
                     YVALQK"
BASE COUNT         3434 a         1109 c         1268 g         2886 t
ORIGIN      
        1 gcaaaatttt tttagcagtt ttgtatttat ttgtatttat tttgaattta tttactaaat
       61 ttgcctataa aaagtatata taaaaatata gttcaaaatg gtataattaa gtataacaga
      121 aggaggtaat gtaagtgagc caatacataa atataaattc tcagttagac gccctaaaat
      181 tgaaggagtt cgagaaaata ttttacgaaa atatcaatga ggacgttaaa gtagtgccaa
      241 aagttactcc ttttaaagga ataaataccg atttgttgta tgttaaagat ggaagaattt
      301 tatttataaa atttatggat acaacagaag atatattttt tattttagaa gaagagttac
      361 ttgaagttat gaatgaggaa tatgaacttt tgaaactaaa aatgggacaa aagaatagaa
      421 atataagtta taactacgtt tacattatgc catacgttga agttgaggaa acatatgaat
      481 ttgaagaatt tgttaataat aatataatag ataaaaataa gcttcaagat ataatgaata
      541 agggttcgct ggaggaatat cttaatgatg agaatgatga aataaactta aatttatttt
      601 tactagacat atgttcagaa tactatataa taaatgataa acttcatcta aatgaaaaat
      661 ttaaaaaaat ttcattctac aatgatgact ataagtatac agcaactatg atggaagacg
      721 ttcaaattaa agatgttgtt tctattaaat atgggaatac acttattgaa ggtggttctg
      781 gtataggcaa aacagcaatt atgctttcta gggctataaa attagcaaaa gtatatccac
      841 atcataaatt agtcatattt acacatacaa aacaactgtg caatgagctt cgagagagga
      901 tagagttatt atacaaagat aataacaatt tagaagttca cacatttagt tcttttatat
      961 ttaaactagc taaaaaattc aatttaattg tagattataa catgttaaaa aatgattacg
     1021 aaaagacctt taataatctt gtaaaacaag cacagaacat tataaaaaat aaaaatatgt
     1081 tcaaggctat ttttattgat gaagcagaaa gctttttaga gtatgaaata gattttataa
     1141 gggagttttt atataaaaca aaattcatat ttaatgtgtg ttcttgtaac tccttgaata
     1201 tctctaatag attgaatata tttaaaaaat tatataatgg tatagaattt gatgataaga
     1261 ttatcttaag taaaaattat agacaagcta aagagattgt agattttaca aataaatttt
     1321 caaataattc taactcatat atcaacgaat taagaccaaa tacagaattt agtactttct
     1381 tttatactaa agcacttaga ggtggaaata agtctgttga tataataaaa gtaagtgacc
     1441 ttgatgacca aataagttct gtaatctggg aaattgagta tttaataagt aaaaaaggtc
     1501 ttgattattc tgaagttgct atcgtttatc cttacaataa gaagaaatta aaaagtggaa
     1561 aaaccattta ctttcaatat atgttaaaaa aagcattgga agaggcaaaa attccttata
     1621 tatgtgcaga agataatttg acaaatataa gtaagaaggt aggaactaca atagcaaata
     1681 tatatgcaat aaagaattta gagtataaag cggtaatagt atgtgaatta gagatgttat
     1741 acaatcaaac tataaatgat attgaacaag attatcaagt aaatgacttt gtaggagatt
     1801 taaataaggt ttatttagct atgagtagag caacagacta cctaagtata gtaacaactt
     1861 ttaatgaaga agcaagtgat ataataagac ttattacaga atcaaaagat atttagtaaa
     1921 aaaatatttg taatttaaga aaataaaaag agactgagat ttgtaaaata aactttacca
     1981 attcagtctc ttatttttta gtctactttc ccaatattat caagaggtaa tagtctcacc
     2041 ataaccttac caagaacttc atcttcatca accataccta catcagggaa tctactatca
     2101 ttactatttt ctctattatc tcccattgca aatagtttac cttttggaac aacagtatct
     2161 atatctccag aagtatagtt attgtgtata tatggttcat ttaataattt tccatttaca
     2221 tacactttag aatttgatat ttttattctg tcaccttcag tagctataac tctttttatt
     2281 aagtcttttt tctttcctgt ttctccatca actaaattgg ttttaaaaac tacaatatcg
     2341 cctttttcag gtttaccaac cttgtatgat atcctattta aaatcagata atctttatca
     2401 tctaaagtag gatacattga ttctccactt acaatagaag gtcttataaa ttgtagaatt
     2461 acaaatgcaa gtacaatagc catagctatt gacttaatcc aatcaaatat ttctttttta
     2521 acactcataa cttttctcct tatcatttgt tattaaatta gaatacatta taatagaaag
     2581 aaataagcaa gaaccactat acccaagact attctataat aaccaaatac tttgaagtca
     2641 tgttttttga tatagtccat aaaaaattta ataactacaa ttgaaacaac aaatgctact
     2701 acagaaccaa cacctaaaat aagccattca aatccagtaa atccaaatcc agcttttaga
     2761 agttttaatg cactagctcc aagcattgta gggatggcta agaagaatga aaactctgct
     2821 gctacatatc tagaagtacc aagtaatact gcacctatta tagtagaacc agacctagat
     2881 gtacctggta taagagctag acactggaat aatcctatac aaagagctaa tttatatgta
     2941 acttgagaaa aatctttata cttaggtttt ttatttctct tttcaagccc tatcattatt
     3001 ataccatata caataagagc aatagcaact actgttgaat taaagaataa tctatctata
     3061 tcatcttcaa acagtattcc aataactcca gatggtataa ctgcaattat aaccttaagc
     3121 cataaacgga ctgtttcttt tttctgctta atattctttg cgctatcaaa aggatttaat
     3181 tttctaaaaa atataacaag tacagctaat attgaaccaa attgaattac aactaaaaat
     3241 gtacttataa atgtatttga aaaattcaat tttataaact catcaactaa tatcatatgc
     3301 cctgtactac ttactggtag ccattctgtt attccctgaa ctatgcccaa aatgactgcc
     3361 ttaaaaattt caagcaaact aaatgtttcc aaatcaaaat acctccaata caaatttata
     3421 gtagcatatt ttattcatag attaataaaa aaatgccacc ttttactaac ttataatacc
     3481 atagtactgt gtagtgcaca atggaagatt gatattttca taaaaccttc ataatataac
     3541 aaaataaact taaaagttat ctattgaata aggggtatat ttgaggggga aatatatggt
     3601 atagtaaaag tatagctatt ttattttatg aaaaggaaag gagttattat gtatagtaca
     3661 aaaatactag atgattatga gtatgaaact tttaatgttt tttctacaaa attaaatcca
     3721 aacacgaaac acgattattt atcaaaggtt atactgttta aagaattttt aaaagggaaa
     3781 gaattgattt atgctacgaa agaagattgt aaaaattttg tagactatat acagactaaa
     3841 tatgcgaaat caacttgtga aaagatatat agttatttac atagttttta taacttctta
     3901 aaaaaagaag gatacataga tataaatcca tttagatatg tagaaaaacc cacagtaaca
     3961 agaataaaaa caaaagatga tgttctaagt atacaagaaa taaataaact tataggtatc
     4021 ctaccaaaat taaatataag agatagagta ataatagtct gtctagttac tactgggtgt
     4081 ttattaaatg aattagttag cttaaagtgg aaagacctta tggtagatga aaacgataac
     4141 tcatatgtaa ggttaggtaa gggaagaaaa gaaagagtag ttaaattaca tccatacttt
     4201 tttaaacttt tagaagatta caggaattat tctgggcttc cagaggtaat aatacctagt
     4261 gatgatttta tttttacaac tcaaaagagt aattctataa cagatagaaa tgtcagactt
     4321 atagtcgaaa aagcattaga tttagctgga ctttctcaat attcagcaag agattttaga
     4381 cattcatttg cagcaataag tttaaggctt ggggcagatg aaagtgatgt aaaaaatcag
     4441 cttggatgga gtgataaata ctatgcaatt agatataagt atgtattaaa ttttgttgat
     4501 agtgaaatag tggattactt aattgaaaaa gataatttgg atataaataa aaaatagtaa
     4561 taaataaaaa atactatata taaatgtgaa caaaaaataa aaaagtcttg ttatagttcg
     4621 taaaattata tataattatt tatgtactaa tgtgtacaca gtaatcacac gacaaaagat
     4681 ttaaggaagg tgctaatata atgatagata ataaatatac tacttactca aacccactta
     4741 cagacagata ttgtagcaag gacatgagtt atatattttc tccacaattt aaattctcta
     4801 catggagaag gttatgggtt gcacttgcag aaggtgaaca tgagttagga atcaatataa
     4861 ctgaagaaca attagatgag ctaagagcta atatagataa tataaatttt gatgaagcta
     4921 gaaaaataga aaaagaagta agacatgatg taatgtctca cgtaaaagct tatggtttac
     4981 aatgtgaaaa agccaaagga ataatacatt taggagcaac atctgcatat gttggagata
     5041 atacagatgt tatacaaatg aatgaagctt taaaacttct aagagtaaag cttgtaaatt
     5101 taataaacaa tttaaaagaa tttgcactta agtacaaaga tatgccagct cttggattta
     5161 cacacttcca agctgctcaa cttacaacag taggaaaaag agcttgttta tgggctcaag
     5221 atttaattct tgatttggaa gatttaaact atagaataga caacatgaag ttaagaggag
     5281 ttaaaggtac tactggtact caagcaagtt ttttaagctt atttgaagga gaccacgaaa
     5341 aagtaaaaga gttagataag aaaatttgta aaaagatggg ataccattct tcctatgcag
     5401 taagtggaca gacatatact agaaagttag attatcaagt aataagcata ctatcaggga
     5461 tagctcaaag tatgcataag atgactaatg atataagact tttacaaagt ttaaaagaat
     5521 tagaagagcc atttgaaaaa aatcaaatag gttcatcagc aatggcttat aagaggaatc
     5581 caatgagaag tgagagaata gcatctttat ctaagtatat aatctctgaa tctataagcc
     5641 ctgcaatggt acaagccact caatggttag agagaacatt agatgactca gccaataaga
     5701 gattggcaat acctcaagca tttatggcag cagatgctat acttgaaata gggataaatg
     5761 taactgatgg tttagtagta tatgaaaata tgataaatag acgtgtaaat gaagaacttc
     5821 cttttatggc tacagaaaca atacttatgg aagctgtaaa aagaggtgga gatagacagg
     5881 aattgcatga aataataaga gaatattcta tgaaagctgc ctatagagta aagcatgaag
     5941 gcaaggataa taacttaatt gaacttataa tgaatgatga ttcatttaaa atgagcaagg
     6001 aagaaatact atctataatg gacccaaaaa actttatagg tagagctcca gaacaagttg
     6061 tagaatttgt aaatgaagtt gtagaacctg ctataaaaga ttataaggaa gacttaggaa
     6121 aagtagatgt agacctaaga gtatagaatt gtacagataa aatataatta aaaacggcta
     6181 tcttatttat aaataagata gccattttaa tgggctagcg tcttctctcc accgctgtga
     6241 agagttcttc tttacttact ataccatgaa tttaataaaa tagctatata aataatattt
     6301 aataggaata atataaataa aaataaaaaa atgataagat tactaattaa atttaaacat
     6361 tcagcaaaaa ctattgtact tgcaatattt ttaatgtaaa ctgaaataaa gttatttaga
     6421 taatttaata aattttaaaa caaccgaggt gattatatga actatatagt aaaagatacc
     6481 ataagttttt tagtaaaaac atttcctaag atttattcta gcctgtactt agaagaccta
     6541 aaaaaatttg caccagatta taatgtaaat aaaacacaat taagagcatt agttttcata
     6601 aaaaattatg gagtaataag tatgactgat ttatgctcta agttaaatat agaaaaaggc
     6661 agtttaacta gtatggttga tgatttaact gataagaagt atgtaacaag gaaaagagat
     6721 ttagttgata gaagaaaata tctaattgac attactgaag agggagataa gatagcaact
     6781 gattttatgg ataaattaag tgatggttta ggagaaaaac tttctaaatt aactgaagaa
     6841 gacagaaaaa aatatttaga agcaataaat actcttcaat atatattaaa caaagaagaa
     6901 tttagataga aaaaatataa aaatttttgt atttaatggt actaagatta attctttgtt
     6961 attcatttaa ctatataatt ataagtctaa atacaatcta aaaagtacat cttaaatttt
     7021 cttgatgtac ttttttataa tttctctaac taaggcaaga caagttttag atgctttaat
     7081 taaagtgaat aaagattact ggccaacact atataaagaa aatgaaaaat agtcaggtct
     7141 aagtcttaat taatatatta attaagaaat gtcccctttt caaataatat ctgaaaaggg
     7201 gacattttat atcattaaat ttatataaat taagtttaaa aaattatttt tgtaaagcta
     7261 catattgctc taaaccttct tttaataatg tagcagctct atgaaggtct ttttcattta
     7321 agatgtaagc taatcttatt tcatcacgtc ctaaacctgg agtagcatag aatccttcag
     7381 cagggcaaac cataactgtt tcattatctt tattaaattc ttttaacatc caaattgtga
     7441 aatcttctgc attttcaact ggtaatttag caactatata gaaagcacca gttggttttt
     7501 tacatataac accttcaacc ttcattaatt cattgtataa tacatctctt ctttttttgt
     7561 actcttcatt aacttctttg aagtaagaaa caggagtttt gtataactca acagctccta
     7621 tttgctctaa tgtaggaaca caaagtcttc cttgacataa tttaagtatt tgagcgataa
     7681 gacctttatt tttgcaagcg atagaaccaa ttctagcacc acaagcactg tatctcttag
     7741 aaacactgtc tattattata actctatcct gtacaccctc taagttacca cagcttgtat
     7801 actcaagccc atcataaaca aattctctat aaacttcgtc tgctattatc cataaatctt
     7861 tttctttagc tatttcagct aggatattaa gttcttcttt tgtataaatt gcaccagttg
     7921 gatttcctgg gttagaaaga attatagcct tagttttatc atctacttta gacaatattt
     7981 cttctttaga tggaaggtgg aaaccgtttt ctgcctttgt tgtaacagca ttaacttcta
     8041 cgtttacaga ttgaccaaat ccattgtagt ttgtatagaa tggttctgga actaatagat
     8101 tatctcctgg gtcacaaact gccatcatag tgaaaagtaa agcttcacta ccaccgttag
     8161 ttactaataa ttcatctttt tcaaaattca tatcgtaagt tttatagtaa ttttgaagtg
     8221 cttctaataa ttctggaata ccttctgatg tagcatattc taagacttcg ctatcaaagt
     8281 ttttaacagc atcaaaaaat cctttaggag tttttatgtc tggttgtcct atgtttaagt
     8341 gatatacttt gattccttta tcttttgctg cttgtgcaaa tggaacaagt tttctgattg
     8401 gagaggcttg cattgcacta actctgtttg aataattcat tttaataccc cctatttaat
     8461 ttaaatacgt atattgtatt atgtaaattg tatgtttgaa tacaagacat ctttacaaga
     8521 taataataac atacttatgg gtaaaaatca tcaaaaaaaa ttaatatagc tttaaaataa
     8581 acttttaaca aattatagta aaatacccat gtttatgcca ctaaattatg tattcataaa
     8641 ttaatttata gtaagataat attctgatgt actgatagtg attttttagt tataatc
//
LOCUS       DAMJYR010000144         8601 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.149, whole genome shotgun sequence.
ACCESSION   DAMJYR010000144 DAMJYR010000000
VERSION     DAMJYR010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8601
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.149"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            124..1983
                     /locus_tag="PC471_003232"
     CDS             124..1983
                     /locus_tag="PC471_003232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HDF2790338.1"
                     /translation="MKRFLRRIILVLFILLIFISFISIKLIHNVGDYGKLINYVGIVR
                     GASQRLTKLEMNHQPNDELIEYIDEILQELITGHGDYGLVITDCNEYNEDLLLLEKKW
                     EDLNIEIKKVRMKEQNNQLLSISEEFFSLANDTVFKIENFSKEKSNYLMTLIIIISII
                     GILACIILILQYSKKMVRLEKLNVDLKNIAYKDELTGVNTIEKFKLDANQNICIHKDK
                     KFAVFYIDFENFKYINDIFGYDYGDMILKRYANLMMNDIGKYEIFAREIADRFVALRC
                     YVDKEDLVVRQRDVDNELINITNEIKNKHSITVVSGICCIEDVNEKLSIDGLINRANF
                     AQKTVKNKPGTNYAFYNDSIRKKMIEENTIKSRIHEAIEKREFIVYLQPKVNLHSQKI
                     NCAEALVRWLTPDKGIISPAIFIPVLEKNFFIALVDKYVFEEVCKWIRKRLDENKPFV
                     QISVNVSRIQFYNTKFVETYSNIQNKYRIPKNTIEIEFTESVAFENQKHLLEIIHDLH
                     ENGFTCSLDDFGKGYSSLSVLKDLPFDVLKLDSMFFKASLDKDKEKIVIKNIVHMLKE
                     LNITTVAEGIECKEQVEFLRDIGCDLVQGFVFYKPMPILEFEKILDKEFVYNS"
     gene            complement(2275..2874)
                     /locus_tag="PC471_003233"
     CDS             complement(2275..2874)
                     /locus_tag="PC471_003233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HDF2790339.1"
                     /translation="MAIELKKGQKINLTKKENSDLGEILVNLNWNQKVQKKGFFGSLR
                     SSNIDLDLGCLFEMKNGVKGAVQALGNAFGSLDNPPFAQLDGDDRTGSNTQGENLRIN
                     GNKIKDIKRILIYAFIYEGVANWSEADGIVTIKQKQDSDLVVKLDEHKNGYNMCSIAL
                     IENVNDETFSVEKVVKYFKGHREMDTEFHWGLKWVAGRK"
     gene            complement(2977..3774)
                     /locus_tag="PC471_003234"
     CDS             complement(2977..3774)
                     /locus_tag="PC471_003234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HDF2790340.1"
                     /translation="MKFKKLLCLLLCLVLTLAVVGCSKAKDDKKIVVGATLVPGGELL
                     EELKPLIKEKGYTLEVKNFDDYILPNEALNNGEIDANLFQHEPYLKEAVKAKGYKIMA
                     GKKLYVCPAILYSYKIKSVDEFKKGDTIAISNNPSSCSKNLRYLESIGLLTLPKGDGL
                     VSPKDIIENPKGIQFKELDIAQIPSSLPDVTAAFIDTTYAVPAGLDAKKNGIYTAPIN
                     DEYANLLAFRTEDKDSEKIKVLQDVLTSDKARSLIEEKYKGIVIPTF"
     regulatory      complement(3887..3992)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            complement(4084..5070)
                     /locus_tag="PC471_003235"
     CDS             complement(4084..5070)
                     /locus_tag="PC471_003235"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HDF2790341.1"
                     /translation="MLLIYNEKTNPYFNLAMEEYLLKNFDEDLFILWRNEPSIIVGKN
                     QNTLSEINLEYIKEYSIPVVRRQSGGGAVFHDLGNINFTFIACNNNNFSDFRRFTQPI
                     IDLLKTLDVNAEFSGRNDLLIDGKKFSGNAQYNYKNKVMHHGTLLFSSEINDLSKALK
                     VKPIKFEGKGIKSVKSRVTNISEHLHSKMDILEFKDAIMDYLSKTNTDNTNYCLSEHD
                     IKQIEKLTKSKYETWDWNFGNSPKYTLSNELKYSGGNVEFNLNVDKGIITSIKFFGDF
                     FGKCDVAFVENKLIGIRHEENALRNTLDSIEINDYFLGADTDILVSGILGVK"
     gene            5554..7284
                     /locus_tag="PC471_003236"
     CDS             5554..7284
                     /locus_tag="PC471_003236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC family protein"
                     /protein_id="HDF2790342.1"
                     /translation="MRNKKINIIFLTTIMFIMSTVMVFAEEDIDTIALANAEKFGILT
                     LIPPLVAIILAFITKNVIISLLIGILSGSFIIKASGINVFATFIQAFLDLVDRALVSL
                     ADPWNAGIILQVLSIGGVINLVAKMGGAKAIAEALAKRAKSAKGTQLITWFLGLLVFF
                     DDYANSLIVGPMMRPVADKMKISREKLAFIIDATAAPVAGLAIISTWIGLEVGLIHDA
                     FESISIDVDAFGIFLNTIPFRFYNILILAFIVISALLLKEFGPMRKAEIKSRSRKISI
                     DLDEGVEELDDLAPKNGVKLSVWNAIIPIGTLIIVALASFYYSGYTSIMGGDDKALIQ
                     LFTNSPYSFEAIKEAFSASDASRALFQSALVASLVAIIMAVVKKIFTISEAIDVWIDG
                     MKSLVITGVILILAWSLSSVIKELGTAKFLIHLLSGSLPPFLLPSLIFGLGAIISFAT
                     GTAYGTMGILMPLAIPLAYSLNPDMSYVIVSTSAVLTGAIFGDHCSPISDTTILSSMG
                     AGCNHIDHVNTQMPYAIFTAVITIVFGYIPAGLGLPIYIVLPVAIAAIFVGIQIIGKK
                     VDEAEIELVE"
     gene            7394..7474
                     /locus_tag="PC471_003237"
     tRNA            7394..7474
                     /locus_tag="PC471_003237"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7428..7430,aa:Leu,seq:tag)
     gene            7809..8324
                     /locus_tag="PC471_003238"
     CDS             7809..8324
                     /locus_tag="PC471_003238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HXXEE domain-containing protein"
                     /protein_id="HDF2790343.1"
                     /translation="MEKYIWLFPLLFIFHDMEEIIGFGIWLKKNKSMLDKKYPFISKV
                     YENYSTEGMAFAVFEEFILCIIFCILTVITENQYVYLLWLGSFIAYTLHLVIHIGQSI
                     IIRKYIPSLITSIICLPISIWCISKSIYIVDCEMSTTILYSIIGIIIVALNLKFAQSL
                     IGKFTKWMSNI"
BASE COUNT         3100 a         1069 c         1311 g         3121 t
ORIGIN      
        1 aacaaggttt atatattcta attgtataga aaaatttatt gtaaacgcat gtatattatt
       61 agaaaaaata gtttggaggt attcaataaa cataaaaaac aatatagggg ataaaggtgt
      121 tttatgaaga gatttttgag gagaattatc ttagttttat ttattttgtt gatttttata
      181 agttttattt ctattaaatt aattcataat gttggagatt atggaaaatt gattaattat
      241 gttgggattg ttcgtggagc tagccaacgt ctaactaaac tagagatgaa tcatcaacca
      301 aatgatgaat taatagaata tattgatgaa atcctacagg agttgattac agggcatgga
      361 gattatggtt tagtaatcac tgattgtaat gaatacaatg aggacttact tttattagaa
      421 aaaaagtggg aagatttaaa tattgaaatt aaaaaagtaa gaatgaaaga acagaataac
      481 cagttattaa gtataagtga agagtttttt tctcttgcaa atgatacagt gtttaaaata
      541 gaaaactttt ctaaagaaaa aagtaattac ttaatgactt taataataat tatatctatt
      601 ataggtatat tagcatgtat catattgatt ttacaatata gtaaaaaaat ggttagactg
      661 gaaaaattaa atgtagattt aaaaaatatt gcatataaag atgaactaac aggagtaaat
      721 actatagaaa aatttaaatt ggatgcaaat caaaatattt gtatacataa ggataagaaa
      781 tttgctgtat tctatattga ttttgaaaac tttaaataca ttaatgatat atttggatat
      841 gattatggtg acatgatact gaaaagatat gctaatctta tgatgaatga catagggaaa
      901 tatgaaatat ttgcaagaga aattgcagat agatttgttg cgttaagatg ctatgtagat
      961 aaggaagatt tagtggtaag acaacgagat gtagacaatg aactaattaa tataacaaat
     1021 gaaattaaaa ataaacatag tattactgtt gttagtggaa tttgttgtat tgaagatgta
     1081 aatgaaaaat taagtataga tggtttaata aatagagcaa attttgcaca aaaaactgtt
     1141 aagaataaac caggtactaa ttatgctttt tataatgaca gtattcgtaa aaaaatgatt
     1201 gaagaaaata ctatcaaaag tagaatacat gaagctattg aaaaaagaga atttatagtt
     1261 tatttacagc ctaaagttaa tttacatagt caaaaaataa attgtgcaga agctttagtt
     1321 cgttggctta ctccggataa aggtattata tctccagcaa tatttattcc agtacttgag
     1381 aagaattttt ttattgcctt agtagataag tatgtttttg aggaggtttg taaatggatt
     1441 agaaagaggt tagatgaaaa taaacctttt gttcagatat cagttaatgt ttcaagaata
     1501 caattttata acactaaatt tgtagaaact tattccaata tacaaaataa atacaggata
     1561 cctaaaaata ctatagaaat tgaatttaca gagagtgttg cttttgagaa tcaaaaacat
     1621 cttttggaaa ttatacatga tttgcatgaa aatggattta cttgttcttt agatgatttt
     1681 ggaaaaggtt attcgtcttt aagtgtttta aaagatttac catttgatgt tttaaagctt
     1741 gattctatgt ttttcaaggc tagtttagat aaagataaag agaaaatagt aataaaaaat
     1801 attgtacata tgttaaaaga acttaatatc acaactgttg ctgagggcat tgaatgcaag
     1861 gaacaagttg aatttcttag ggatattggg tgtgatttag ttcagggatt tgtattttat
     1921 aaaccaatgc caatattaga atttgaaaaa atattggata aagagtttgt ttataatagc
     1981 taaaatgtac tttataaaat atgaactaat taagtaaaat tattttgtac tgaataaatt
     2041 tttactttat ttacagaata atgattattt acagaaaagc gcgaaaagtg tattccttta
     2101 agtgtgggat ggatagcaca aatagaggag aaaaaatatt ggccatataa gaatgcataa
     2161 taatataaat atttaaaagg tcagcattat ataaaaatta aaaagaaaat aaataatttt
     2221 tctataaaaa agagactatg agaaaattta ctcatagtct cctaaataat gtatttattt
     2281 tcttcctgca acccatttta atccccaatg aaattcagta tccatttctc tatgaccttt
     2341 gaagtattta actacttttt caacactgaa tgtctcatca tttacatttt ctatcaatgc
     2401 tattgaacac atattatagc catttttatg ttcatcaagt ttgacaacta agtctgaatc
     2461 ttgtttttgt ttgattgtta caattccatc agcttcagac cagttagcta caccttcgta
     2521 tataaaagca taaataagta ttctttttat atcttttatt ttattaccat ttattcttaa
     2581 attttctcct tgtgtattac ttcctgttct gtcatctcca tctaattgag caaaaggtgg
     2641 attgtctaaa ctaccaaagg catttcctaa tgcttgaaca gcacctttta caccattttt
     2701 catctcaaat aggcaaccca aatctaaatc tatattactg cttcttaaag agccaaaaaa
     2761 accctttttt tgtacttttt gattccaatt taaattaact aaaatttcac ctaaatcgct
     2821 attttccttt tttgtaaggt ttattttttg tccctttttt aattctatag ccatagtaat
     2881 cttcctccac aaacatagat ttatttaaat ggtagcactt aataagtcaa tattcaacaa
     2941 gattagaata ataatattaa aattatacta aatatactaa aaagtaggaa tcacaatacc
     3001 tttgtatttt tcttcaatta agcttcttgc tttatctgat gtaagaacat cttgtagaac
     3061 tttaatcttt tcactgtctt tatcttcagt tctaaatgct aaaagatttg catactcgtc
     3121 atttatagga gctgtataaa ttccgttttt cttggcatca agaccagcag gtacagcata
     3181 agtagtatct ataaatgcag cagtcacatc tggaagagaa gatggaattt gagcaatatc
     3241 taactctttg aattgtattc cctttggatt ttctattatg tccttaggac taactaatcc
     3301 atccccttta ggtaacgtta aaagtccaat actttcaagg tatctaaggt tcttagaaca
     3361 agaagatggg ttattgctta ttgcaattgt atctcctttt ttaaattcat caacagattt
     3421 tatcttataa gagtaaagta ttgctgggca tacatatagt tttttaccag ccattatttt
     3481 gtaacctttg gccttaacag cttcttttag ataaggttca tgttgaaata gatttgcatc
     3541 aatttcacca ttatttaaag cttcgtttgg aagtatgtaa tcatcaaagt ttttaacttc
     3601 taaagtatat cctttttctt taataagtgg ttttaattct tctaataact ctcctcctgg
     3661 aactaaagta gcaccaacaa caattttttt atcatcttta gctttagaac atccaaccac
     3721 agctaaagtt agtactagac ataataacaa acataataat tttttgaatt tcatataaat
     3781 tccctcctga atataattga ttagttaaaa ataaaatacc attctttatc acactgcaaa
     3841 ataaaaataa gcctgagttt aaactcaggc tttaaaagct aatttagact catctttcag
     3901 tataatactg caggatttag caccaatgta tataaaaata tactggttgc tgggtttcat
     3961 agggccagtc ccttcaccac tctggataag aagtatcaat ttttaattat gttataaata
     4021 ttatcatgat tttttacgtc tgtaaacata attttataaa tattttaaaa tctttaatgt
     4081 attttattta acacctaaaa ttccagaaac tagaatatca gtatcagcac ctaagaaata
     4141 gtcatttatt tcaatagaat ccagagtatt tctcaaagca ttttcttcat gccttatacc
     4201 aataagttta ttttctacaa atgcaacatc acattttcca aaaaaatctc caaaaaattt
     4261 tatactagtt attatgcctt tatcaacatt taaattaaac tcaacatttc caccagaata
     4321 ttttaattca tttgaaagag tatattttgg tgaattacca aaattccaat cccaagtttc
     4381 gtattttgat ttagtaagct tttctatttg ttttatatca tgctcactca aacaataatt
     4441 agtattatca gtatttgttt tagaaagata atccattata gcatctttaa attctaatat
     4501 gtccattttt gaatgtaaat gttcacttat attggttaca cgagatttta cagattttat
     4561 tccttttcct tcaaatttta taggtttaac ctttaatgct tttgagaggt catttatttc
     4621 agatgaaaat aaaagagtcc catgatgcat tactttgttt ttgtagttgt attgagcatt
     4681 tccagaaaat ttcttgccat ctataagaag gtcatttctt ccagaaaatt cggcattgac
     4741 atctagtgtt ttaagtaaat ctattatggg ctgagtgaat cttctgaaat cactaaaatt
     4801 attattgtta caagctataa atgtaaaatt tatatttccc aaatcatgaa atacagctcc
     4861 accaccagac tgtctcctaa cgacaggtat agaatattct tttatatatt ctaaatttat
     4921 ttcagaaagt gtgttttggt tttttccaac aattatagaa ggttcattcc tccataaaat
     4981 aaataagtct tcatcaaaat tttttagaag atattcttcc attgctaaat taaaataagg
     5041 gtttgttttt tcattataaa ttaataacat taaagtagtt cctccataga attgataaaa
     5101 tttttattat gaatacaatt aattttataa ttcgacaata atataaaaac tcctttaaat
     5161 tagaaatttt aaaaactacc ttgcaaagtt taaataaata atatatactc aaaatatgtt
     5221 taataaaagt gaatagaagg gtagaggcgc aataatctat cagtactaat aaggagttaa
     5281 gcacagagag ttattaggaa aggagatatt gccgaagttt tataatcgat gctttaagat
     5341 tatattgctg ggggtacata gaaaatatgt acaactgtca caggtttatt tgtggtgagc
     5401 tatcatttaa ggaattatat tatatttatt tttactattg ttgatatatt gtaatgttta
     5461 agccttgagt gtttaccact caaggctttt ttagttttat tgtaacataa taccctgtac
     5521 agttcacaaa attaaaagga gagggagatt tatatgagga acaaaaaaat taatattatt
     5581 tttctgacga caatcatgtt cataatgtca acagtaatgg tttttgcaga agaagatata
     5641 gacactattg cactagccaa tgcagaaaaa tttgggattt taactttaat accaccatta
     5701 gttgctataa tactcgcatt tataactaaa aacgtaataa tttctttact tataggtatt
     5761 ctttctggta gttttataat taaagctagt ggtattaatg tttttgcgac atttatacag
     5821 gcatttttag atttagtaga tagagcttta gtatctttag cagacccttg gaatgcgggg
     5881 ataatactac aggttttgtc aattggagga gtaataaatc ttgttgctaa aatgggagga
     5941 gctaaagcta tagctgaagc acttgctaaa agagcaaagt cagcaaaagg gacacagctt
     6001 ataacatggt ttttggggct tttagtattc tttgatgatt atgccaattc actaatagta
     6061 ggtcctatga tgagacctgt tgcagataaa atgaaaatat cgagagaaaa gctagcattt
     6121 ataatagatg ccacagcagc accagttgct ggtttagcta taatatctac atggataggt
     6181 cttgaggttg gtttaataca tgatgctttt gaaagtataa gtatagatgt agatgcattt
     6241 ggtatatttt taaatacaat accatttaga ttttacaata tattaatatt agctttcatt
     6301 gtaatatcag cattattatt aaaagaattt ggtccaatga gaaaagctga gataaaatct
     6361 agaagtagaa aaattagtat agaccttgat gaaggcgtag aagagttgga tgatttagct
     6421 ccaaaaaatg gagtaaaatt gagtgtatgg aatgctataa tcccaatagg aacattgata
     6481 atagttgcat tagcatcatt ttattatagt ggatatacat ctataatggg aggagacgat
     6541 aaggctctga tacaactatt tacaaattct ccatattctt ttgaagcaat aaaagaagca
     6601 tttagtgcat ctgatgcatc tagagcatta ttccagtctg cacttgttgc aagtttagtt
     6661 gctattataa tggctgtagt aaaaaagatt tttacaatat cagaagcaat tgatgtttgg
     6721 atagatggaa tgaaaagttt agtaatcact ggtgtaatat taatacttgc atggtcatta
     6781 agttcagtaa taaaagagtt aggtacagct aaatttttaa tacacctact atcaggttca
     6841 ctaccaccat tcttattacc aagtttaata tttggtttag gagcaataat atcttttgct
     6901 actggaacag cttatggaac aatgggtata cttatgccac ttgcaattcc attagcatac
     6961 tcattaaatc ctgatatgtc ttatgtaata gtaagtacta gtgcagtttt aacaggtgca
     7021 atatttggag accattgttc tccaatttca gatacaacaa tactttcttc aatgggagct
     7081 ggctgtaatc atatagacca tgttaacact caaatgccat atgctatatt tacagcagta
     7141 atcactattg tatttgggta tataccagct ggacttggat tgccaatata tatagtatta
     7201 ccagtggcaa tagctgctat atttgtaggt attcaaataa ttggtaagaa agttgatgaa
     7261 gcggaaatag aacttgtaga ataaagatat aaataagttt caccttttat tatcactatt
     7321 ttgttagttt ttagataatt ttaaaaagtt attgaaaata atggcgaaat atagtaaaat
     7381 aaaagagtga taagcggatg tggcggaatt ggcagacgca ctagacttag gatctagcgc
     7441 cattggcgtg ggggttcgac tcccttcatc cgcactttac agtacttaat tgtactgaaa
     7501 aaaactgggg ttgctggctt tcggagtggg ctatcccgtt tttatttttt tgtatatata
     7561 atagattggc actttagtat aaggtgcttt ttttataggc aaaaacagta catttatttc
     7621 tgttttatta tcattcatct attagaatat actttagtta agaatgtgtt aataagcagt
     7681 gttcacattc ttgataaaat gtggaataat atgtattttt actaagacgg aaatgaaata
     7741 attaaaccat taaataatat tataagatga tgaataagta gataaatcaa attttgtagg
     7801 aggatattat ggaaaaatat atatggttgt ttccattact ttttattttt catgatatgg
     7861 aagagataat aggttttgga atatggctta aaaaaaataa atcaatgctt gataagaagt
     7921 atccatttat cagtaaagtt tatgaaaatt atagtactga aggcatggca tttgctgtgt
     7981 ttgaagaatt tattttatgc attattttct gtatacttac agttataaca gagaatcagt
     8041 atgtatatct actatggtta ggtagtttta tagcatatac cttacatttg gtaattcata
     8101 tagggcagtc aatcataata agaaaatata taccttcact tattaccagt atcatatgct
     8161 taccaataag catttggtgt ataagtaaga gtatttatat agttgattgt gaaatgagta
     8221 caacaattct gtacagtata attggcatta taattgtagc attgaattta aagtttgcac
     8281 agtcgctgat tggaaaattt acaaagtgga tgtcaaacat ataaatttta gtttattgga
     8341 cagttcaagg aatcagaaat tggaatttta tagtattaat attttatggt attaatataa
     8401 tgtataaaat tttatagcaa tgtatttcat aagtataaag tgactagaat tttaagaaat
     8461 atacaaattc tataaaaact aaataatttc aagtatattt taaaaatctt atttttcaca
     8521 agtttaaaat tgtaaacgat ttcatcaagt ctatgaatat acttttaaca tgttatagta
     8581 aatctcaata taaatttaca t
//
LOCUS       DAMJYR010000145         8466 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.150, whole genome shotgun sequence.
ACCESSION   DAMJYR010000145 DAMJYR010000000
VERSION     DAMJYR010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8466)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8466)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8466
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.150"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            251..1237
                     /locus_tag="PC471_003239"
     CDS             251..1237
                     /locus_tag="PC471_003239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDF2790344.1"
                     /translation="MKKITINDIANLAGVSKSTVSRYLNNKDISDSTKEKIKTIIDEY
                     GYEPNAFAQSLRAKKTYFIGIITPCLDSFVKSKIMMAIDEELKELKYTSLIINTNRKI
                     RSEIDSISKLASLKVDGIILIGTEITKEHKNEIEKLDIPIVVVGQKVDGINSIVNDDY
                     GAGYKMGQYIANKGYKNIVYLGVDESDISVGLNRKNGVLNGLKDKGYDAKVFYTDFDQ
                     ETSIQRSGEMLESENPDIIICATDNIAIATMKEINKRGKNIPQDISVAGFGGYDVLSI
                     ISPKLTTIKFENKNAGKVAANTIVNLIQERKEPLLKEIKFELIEGESTINKN"
     gene            1406..1554
                     /locus_tag="PC471_003240"
                     /pseudo
     CDS             1406..1554
                     /locus_tag="PC471_003240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423822.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIB"
     gene            1573..3054
                     /locus_tag="PC471_003241"
     CDS             1573..3054
                     /locus_tag="PC471_003241"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HDF2790345.1"
                     /translation="MYKEPKYRTILESTKEELNKLRNISLNDKYKPLFHIHPQHGLLN
                     DPNGLAYYNGKYHVFYQWYPYDATHGMKHWAYVSSDDFVNWNREDVALIPIESYESHG
                     AYSGNAIEVDGKLHMYYTGNIKYSAEDRYAYQNLAIMNKDGKITKYENNPIVSEIPKG
                     YTGHVRDPKVFKRKDKYFMLLGAQTSDKKGAIIVYESKNSIDWNFKGELNVKNIDEDF
                     GYMWECPDYINIDEKDILIFSPQGVEPKGFDYQNIYNVVYAIGNMDLDNLTFEIDTMK
                     ELEKGFDFYAPQTFIKDSQIILFAWAGMGEVLYPTDKNKWAHCLTVPRKLNIKNNKLL
                     QMPVDELIKLRYDETSGQNTIKNNINIIENDENVYELNINIKNIDSNKFGLELFSSQD
                     EGVKLEFNKLGNIVTLDRSNFKKVFGVEYGTNRKEYINIDENTNIKVLADRSILEIFI
                     NEGEVVFTSRIFAKENSNQIRVYSDKIVEYEYTKFKLKQGIEL"
     gene            3080..4051
                     /locus_tag="PC471_003242"
     CDS             3080..4051
                     /locus_tag="PC471_003242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase"
                     /protein_id="HDF2790346.1"
                     /translation="MKKVISIGEALIDFIPNQNGGKLKNVSEFRRVAGGAPANVSAVV
                     AKLGGKSSFISKLGKDAFGDYIIDVLNEVNVNTDYVLRTSKANTGLAFVSLKEDGNRD
                     FSFYRNPSADMLLEADEVKKEWFNNCHILHFCSVDLIDSPMKLAHKKAIEYALESNSI
                     ISFDPNIRLPLWDSEQSCKKAISEFLPFAHIVKISDEELEFITGENDIEKSLHKLFVG
                     NVELVLYTKGKDGVDAYTKKVKGMCKGVKVNAIDTTGAGDSYIGSFLYTLLYKQITLN
                     DIKEMKQETLNEYLEFSNYYAARSTTIKGAISSYATKEEITEFIKNL"
     gene            complement(4307..4909)
                     /locus_tag="PC471_003243"
     CDS             complement(4307..4909)
                     /locus_tag="PC471_003243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HDF2790347.1"
                     /translation="MEVCILMGSPKKKGNTAAILKPFIEELENYNSNIEVIWLYDCKI
                     EPCIACKKCQDNLFDFGCYCKDDVQKIFKKILMCDLIVFATPIYSWYCTSPMKALLDR
                     LVYGMNKYYGNEKGPTLWAGKPVALITTCGYRPEKGADIWENGMKRYCKHSQLKYIGM
                     LVERDLGKPNFMNKEKEEHSRQFAKQIYYILDNSIEVDNC"
     gene            5190..6401
                     /locus_tag="PC471_003244"
     CDS             5190..6401
                     /locus_tag="PC471_003244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HDF2790348.1"
                     /translation="MYSNEDYKFIEENAPCYIYDETNIIEKCKELKNNILGFDFLYSI
                     KTNPYNKIVKSIAEQGFGADASSTNEVMISSECGVSKDDIYYSSPGKTEIDIETVLDK
                     CIIIADSITEIELINKISKKYNQKVKIGIRINPDFTMDDDSAKSSKFGIDIEDMSSVF
                     KMLSMCDYVSVVGIHIHIKSQVLDFEKLGHYYEKCFFIALNLNKSKYINIEFINFGSG
                     LGVAYDNLRENPLNLKELSKLTQKIAFTNSNTLKSRLLIESGRFIVCKSGNYYVKIVD
                     IKVSRGKKYLIVKNGLNGFMRPIISNLIENITCGVELEGQEPLYTSNHAFSLSVLNNL
                     KEEEYVNVVGNLCTSLDVIANNVKLKKANIGDLISISNAGSYSYSLSPLLFSSYEFPK
                     QYYKRKDGYIE"
     gene            6469..6666
                     /locus_tag="PC471_003245"
     CDS             6469..6666
                     /locus_tag="PC471_003245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter"
                     /protein_id="HDF2790349.1"
                     /translation="MELWKRNLFVCWIGMFFSSIGMSQIAPILSLYIKQLGVTDVSLI
                     QQYSGIVFGCTFVVAAFFSPI"
     gene            6700..7671
                     /locus_tag="PC471_003246"
     CDS             6700..7671
                     /locus_tag="PC471_003246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDF2790350.1"
                     /translation="MLLRASIGMGIVIFTMAFVQNVYQLLGLRILQGVFTGYATACTT
                     LIATQTDKNHSGWALGTLATASTTGSLIGPTVGGYIESILGLKSTFIITGGLLFVSFI
                     ISILFVVDNFKPKEIKESISIPKEQLNILPNKFLIASIFVTTFITQLALYSIEPIVTI
                     YISQLTNFASNVALIAGLTFSASGLANLLAAQKLGKMSDKIGPQKVLLISLLWAGIIF
                     IPQAFVKTAWQLMLLRFLLGLSVAGLNPSVNSLLKKIAPEEYVGKIFGYNASAQYIGC
                     SSGAFLGGQISAHLGIRTVFFSTSLLLFMNALWMYKFTGLFIKDKTK"
     gene            7858..8235
                     /locus_tag="PC471_003247"
     CDS             7858..8235
                     /locus_tag="PC471_003247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HDF2790351.1"
                     /translation="MTISKIPQAELKVMKFIWEKNDTVTSKETIEAMERKYGWKQTTT
                     LTLLSRLVKKHFLSAEKIDRYTHYTIVVGHKEYLSVETKDFLSNIHDNSLQSLISALH
                     DDEVVDKDMLDLFESHFKNLEEE"
BASE COUNT         3445 a          882 c         1357 g         2782 t
ORIGIN      
        1 tcatagcctt atttttatat tataaataaa ttagagtatt tatcaattca tagtagataa
       61 aattgaaatt aaatataaca acaaaaaatg aaaattacaa ttaatttaat gttaaagact
      121 caattatttg gatgtggaat ggatagtaca agtagtatat aaaaagtatt gactggacaa
      181 ataactatat tttattttaa atatataata taatgtaata aaatatatca aatgtagaag
      241 gtgataacct atgaaaaaaa taacaataaa tgatattgct aacttggcag gagtatctaa
      301 aagtactgta tcaagatatc taaataataa agatatcagt gattctacta aagagaaaat
      361 aaagacaata atagatgaat atggatatga gccgaatgca tttgctcaaa gtttgagagc
      421 taaaaaaaca tactttattg gtattataac accgtgttta gattcttttg taaaatctaa
      481 aattatgatg gcaatagatg aagaactcaa agaattaaaa tatacatcac taattattaa
      541 tactaataga aaaataaggt cagaaataga tagtatatca aaattagcta gtctaaaagt
      601 tgatggaata atactaattg gtaccgaaat aactaaagaa cataagaatg aaattgaaaa
      661 gttagatata ccgatagttg tagttggaca aaaagttgat ggtatcaata gcatagtgaa
      721 tgatgattat ggagcaggat ataaaatggg tcaatatatt gctaataaag gttacaaaaa
      781 cattgtatat ttaggtgttg atgaaagtga tatatcagtt ggtttaaata gaaaaaatgg
      841 agtacttaat gggttaaaag ataaagggta tgatgcaaaa gtattttata ctgactttga
      901 ccaagaaacg tctatacaaa gaagtggaga aatgctagaa agtgaaaatc cagatataat
      961 aatatgtgca actgataata tagcaatagc aacaatgaaa gaaatcaata agagaggtaa
     1021 aaatatacca caagatatat ctgtagctgg atttggggga tatgatgtat tgtctattat
     1081 aagccctaaa cttacaacaa taaagtttga aaataaaaat gcaggtaaag tagcagcaaa
     1141 tacgatagta aatttaatac aagaaagaaa agaaccacta ttaaaggaga ttaaatttga
     1201 attaatagaa ggtgaaagta caataaataa aaattaagat acacattaca tatattagta
     1261 atgtgtattt ttttactaaa aaaactataa ttctaaaaat taggaaaact tttttgaaag
     1321 aaatattgca tctgaaataa atttatgcta atctttaatt aaaccggttt cgaaaccggt
     1381 ttaattaaac cgatagaata gcaaaatgag tgcaaaaata actgattgta acaaagtatc
     1441 aaagtaatta ttagagtaca taggaataaa caaaatataa aagatgtagc acattgtgta
     1501 ataagactaa gaattgtttt agatagcaat aataaagcaa atataaaatt ataaaagaaa
     1561 aggaataaaa atatgtacaa agagcctaaa tatagaacca tattagaatc gacaaaagaa
     1621 gaattaaata aattaagaaa tatatcttta aatgataaat ataaaccact atttcatata
     1681 catccacaac atggactact taatgaccca aatggattag catattataa tggtaagtat
     1741 catgtatttt atcaatggta tccatatgat gcaactcatg gaatgaagca ttgggcttat
     1801 gtaagttctg atgattttgt aaactggaat agagaagatg tagcattaat accaatcgaa
     1861 agttatgaat ctcatggagc atattcaggt aatgcgatag aggtagatgg aaaattgcat
     1921 atgtattata cagggaatat aaaatatagt gcagaagata gatatgcata tcaaaactta
     1981 gctataatga acaaggatgg taaaataact aaatatgaaa ataatccaat agtaagtgaa
     2041 ataccaaaag gatatacagg acatgtaaga gacccaaaag tatttaaaag aaaggataaa
     2101 tactttatgt tacttggagc acaaacaagt gataaaaaag gagctataat agtatatgaa
     2161 tctaaaaact ctatagattg gaattttaaa ggagaattaa atgtaaaaaa tatagatgaa
     2221 gattttggtt acatgtggga atgtccagat tatattaata tagatgaaaa agatatatta
     2281 atattttcac cacaaggagt agaacctaaa ggatttgatt atcaaaatat atataatgta
     2341 gtatatgcta ttggtaatat ggatttagat aatttaacat ttgaaataga tacaatgaaa
     2401 gaactggaaa aaggatttga tttctatgct ccacaaacat ttataaagga ctcacaaata
     2461 atactatttg catgggctgg aatgggagaa gttttatacc ctacagataa aaacaaatgg
     2521 gcacattgtc taactgttcc aagaaaatta aatataaaaa ataataagct attgcaaatg
     2581 ccagtagatg aattaattaa attaagatat gatgaaacaa gtgggcaaaa tactataaag
     2641 aataatatta atattataga aaatgatgaa aatgtatatg agcttaatat taatataaaa
     2701 aatattgaca gcaataaatt tgggctggaa ttattttcat cacaagatga aggtgtaaaa
     2761 ttagaattta acaaattagg aaacattgta actttagaca gaagcaactt taaaaaagtt
     2821 ttcggtgttg aatatggaac aaatagaaaa gaatatataa atattgatga aaatactaat
     2881 atcaaagtac tagcggatag aagtatttta gaaattttta taaatgaagg agaagtagta
     2941 tttactagta gaatatttgc taaagaaaat tctaatcaaa taagggttta tagtgataaa
     3001 atagtagagt atgagtatac taaatttaaa ttaaagcaag gtattgaatt ataaaaacac
     3061 attataaggg gtagaaaaga tgaaaaaagt aattagcata ggagaagctt taatagactt
     3121 tataccgaat caaaatggtg gtaaattaaa aaatgtatca gaatttagaa gagtagcagg
     3181 aggagctcca gcaaatgtta gtgctgttgt agcaaagctt ggaggaaaat cgagttttat
     3241 atctaagtta ggaaaagatg catttggtga ttatataata gatgtattaa atgaagtaaa
     3301 tgtaaatact gattatgtat tgagaacaag taaagcaaat acaggacttg cttttgtatc
     3361 attaaaagaa gatggtaatc gtgatttctc attttataga aatccaagtg cagatatgct
     3421 tttagaagct gatgaagtta aaaaagagtg gtttaataat tgtcatatcc tacatttttg
     3481 ttcagtagat ttaatagata gtccaatgaa attagcacat aaaaaagcaa tagaatacgc
     3541 acttgaaagt aatagtataa taagctttga ccctaatata agattacctt tatgggatag
     3601 tgagcaatct tgtaaaaagg caatatcaga atttttacct tttgctcata ttgttaagat
     3661 atctgatgaa gaattagaat ttataacagg agaaaatgat atagaaaaat cattacataa
     3721 actctttgtg gggaatgttg aactagtatt atatacaaaa ggaaaagatg gagtagatgc
     3781 atacactaaa aaagtaaaag gtatgtgtaa aggggttaaa gtaaatgcaa tagatacaac
     3841 cggagcagga gattcttata taggttcatt tttatacacc ttattgtata aacaaataac
     3901 attgaatgat ataaaagaaa tgaaacaaga aacgttaaat gagtatttag aattttctaa
     3961 ttattatgca gctcgtagta caactattaa aggtgcgata agttcatatg caacaaagga
     4021 agaaataact gaatttataa aaaatttata gtaggaatga gtaataggtt ttgaaggaag
     4081 taacatgtaa agagttttta aggatgttta ctatgaaaat aattaacata taaaaagcaa
     4141 taaagaggct gtctcaaaat agagataagc ctctttatca ttgttgaaat gataaaaaat
     4201 attttaatta ttgttacttt ttagaagaat atatatctta ttttaaataa gcaaattatg
     4261 taagatttat ttatattatc aatactttac ttggagtatt atgtctttag cagttatcta
     4321 cttcaattga attatctaaa atataataaa tttgttttgc aaattgacga gaatgttctt
     4381 ctttttcttt attcataaaa ttaggtttac ctaaatcacg ttcaacaagc attcctatat
     4441 attttaactg tgaatgctta cagtagcgct tcataccatt ttcccaaata tcagcaccct
     4501 tttcaggtct atatccacat gttgtaataa gtgctactgg ttttcctgcc catagagtgg
     4561 ggcctttctc attaccatag tatttattca ttccatacac caatctatca agtagtgctt
     4621 tcattggaga agtgcagtac caagaataaa ttggagtagc aaaaacaatt aaatcacaca
     4681 taagaatttt cttgaaaatt ttctgaacat catctttaca ataacatcca aaatcaaata
     4741 agttatcctg acatttttta caagcaatac atggctctat tttacaatca tataaccata
     4801 ttacttcaat atttgaatta taattttcta attcctcaat aaaaggtttt aaaatagcag
     4861 cagtattacc ttttttctta ggacttccca taagaatgca tacttccata ttgatacctc
     4921 cacagtattt taaaatagtt tttagtgctt aatatagttt taggatacta tgagaatacg
     4981 ttaacaacaa tacactttgt atatttgtaa taaaattgaa acaaattgtt ttatcaataa
     5041 aagttggaat gtatctataa ttaaaaatat tttaggtatg tttttacata tatgtgtgta
     5101 gttaactatg gtaatttatt gtaaaattat cttataggat tataattgct taatttatga
     5161 ttatatttaa aatcttggag gtaaaaaata tgtactcaaa tgaagactat aaatttatag
     5221 aagaaaatgc accatgttat atatatgatg agactaatat aattgaaaaa tgtaaggaat
     5281 tgaaaaataa tattttaggt tttgattttt tatattctat aaaaacaaat ccatataaca
     5341 aaattgtaaa aagcattgca gagcaaggtt ttggagcaga tgcttcttca actaatgaag
     5401 ttatgatttc atcagaatgt ggagtttcta aagatgatat ttattattca tcaccaggca
     5461 aaacagagat tgatatagaa actgttttag ataaatgcat aattattgca gatagtatta
     5521 cagaaataga acttataaat aagatatcta aaaagtataa tcagaaagtt aaaataggaa
     5581 taagaataaa tcctgatttt actatggatg atgatagtgc taaatcaagc aaatttggta
     5641 tagatataga agatatgagc agtgttttta agatgttaag tatgtgtgat tatgtatctg
     5701 tagttgggat tcatatacat attaaatctc aggtattgga ttttgaaaag ttaggacatt
     5761 attatgaaaa atgttttttt atagctttaa atttaaataa aagtaagtat attaatattg
     5821 aatttataaa ttttggtagt ggacttggag tagcttatga taatcttaga gaaaatccac
     5881 ttaatcttaa agagctttca aaattaactc aaaagattgc ttttactaat tcaaacacac
     5941 taaaatcaag acttcttatt gaatcaggac gtttcatagt ttgtaaatca ggtaattatt
     6001 atgttaaaat tgtagatatt aaagtatcaa gagggaaaaa atatcttatt gtaaaaaatg
     6061 gacttaatgg atttatgaga cctattattt ctaatttaat tgaaaatatt acttgtggag
     6121 tagaattaga aggtcaagag ccattatata catcaaatca tgccttttct ctttcagtat
     6181 taaataattt aaaagaagaa gagtatgtaa atgttgttgg gaatctctgt acatcattag
     6241 atgtaattgc aaataatgtt aaattgaaaa aagcaaacat aggagatttg atttcaatta
     6301 gtaatgctgg aagttactcc tattctttat caccgctatt attttcaagt tatgaatttc
     6361 ctaaacaata ttataaaaga aaagatggat atattgagta aacaatagca taaattaata
     6421 aacttagtat tattgaaaaa atttaaaatg cagggggtgt aattctatat ggaattgtgg
     6481 aaaagaaatt tatttgtatg ttggataggt atgtttttta gttctatagg gatgagccaa
     6541 atagcaccta tactttcatt atatattaaa cagttggggg taactgatgt tagtttaata
     6601 caacagtatt caggtatagt ttttggatgt acctttgtag tagcagcatt cttttcgcca
     6661 atttgaggga aagctgctga taaatatgga agaaaatcaa tgcttttacg tgcaagtatt
     6721 gggatgggaa tagttatatt taccatggcg tttgttcaaa atgtctatca gttattagga
     6781 ttaagaatac ttcaaggagt ttttacgggt tatgctactg catgtactac tcttattgca
     6841 acacagacag ataaaaatca ttcaggatgg gctttgggta ctcttgcaac tgcatcaact
     6901 actggctcat taattggtcc aacagtagga ggatatatag aatcaatctt aggtcttaaa
     6961 agtacattta ttataacagg aggattgttg tttgtatcat ttatcatctc tattttattt
     7021 gtagtagata attttaaacc taaagaaata aaagaatcaa tttctatacc taaagaacaa
     7081 ttaaatatat tgccaaataa atttttaatt gcatctattt ttgtaaccac attcattact
     7141 caactagctt tatactctat agaaccaatt gtaaccatat atatatcaca acttacaaat
     7201 tttgcatcaa atgtagcttt aatagctgga ttgacatttt ctgcatcagg attagcaaat
     7261 ttattagcag ctcaaaaact tggaaaaatg tcggataaaa taggtcctca aaaagtattg
     7321 ttaatttcac ttttatgggc tgggattata tttataccac aagcttttgt aaaaactgca
     7381 tggcaactga tgttgttgag atttttatta gggttgtcag ttgcagggct taatccttct
     7441 gttaacagct tgcttaaaaa aattgcacca gaagaatatg tgggaaaaat atttggatat
     7501 aatgcatctg cccagtatat aggctgttca tcaggagcat ttttaggggg acaaatatca
     7561 gcacatttag gtattagaac agtatttttt tcaacaagtt tattgttgtt tatgaatgca
     7621 ctttggatgt ataaatttac tggattgttt attaaagata agactaaata atcatactat
     7681 ttacatttct aattttatga tgtaaaataa atatttttta tgtaatttat catagaatta
     7741 tataaaaaat aaccctatgg taaaaattgg aaaatattat tatcgacaaa atctttgact
     7801 taaaaactat attgcgatag tattaattta ataaaagttt gagaggggcg taaggttatg
     7861 acaatttcaa aaataccaca agcagaatta aaagttatga agtttatttg ggaaaaaaat
     7921 gatacagtaa catcaaaaga aactattgaa gctatggaac gaaaatatgg ttggaaacaa
     7981 acaactacat tgacactttt atcaagactt gttaaaaaac attttttaag tgcagaaaaa
     8041 atagatagat acactcatta tacaatagtt gttggacata aggagtattt aagtgttgag
     8101 acaaaagatt ttttaagcaa tatacacgac aattctttac agagtttaat ttcagcacta
     8161 catgatgatg aagttgttga taaagatatg ttggatttat ttgaaagtca ttttaaaaat
     8221 ttagaagaag agtaattata tcaggataga actctaagaa tttaattaat ctatggctga
     8281 aaaccataaa tggtattatt taatcagcta ctcactattc ataaagtaat attaaatagg
     8341 ttctctaatt agtaaaatgc ctaaaataat tggaataggg agtatcaagt taaatgatac
     8401 cacttattta aataagaaat gatttttaaa atttaaaata attttataac ttgtcaatat
     8461 tcatta
//
LOCUS       DAMJYR010000146         8442 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.151, whole genome shotgun sequence.
ACCESSION   DAMJYR010000146 DAMJYR010000000
VERSION     DAMJYR010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8442)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8442)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8442
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.151"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            141..1022
                     /locus_tag="PC471_003248"
     CDS             141..1022
                     /locus_tag="PC471_003248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase"
                     /protein_id="HDF2790352.1"
                     /translation="MVKKRKKKTVTLTILVVIVIGIFSMAWADMSRKKEMKETQERLK
                     AERLEKERIEKEKNEPIIGAKKEAVKEYGYDAKIVWDKLNKYDYSNNGEKIVFLTFDD
                     GPSTTNTPQVLDILKRHGVRGTFFIKGDSLERKGANEILKRTFDEGNAIAHHSYTHDY
                     KKLYPNRSLNLDTFVNELNKTDEAMKKVLGKNFSSNVVRCPGGYMSWKNMEPLGNYLK
                     EKNMASIDWNALNADAEGKKKNAQELFEHAKKSSEGKEMVVLLMHDTYGKQETVNALD
                     QIITYFKDNGYQFKILI"
     gene            complement(1083..2291)
                     /locus_tag="PC471_003249"
     CDS             complement(1083..2291)
                     /locus_tag="PC471_003249"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HDF2790353.1"
                     /translation="MKSIDEQMRIIMKGVDDLIDEKELREKLIKSEKEGKPMIVKLGL
                     DPSAPDIHLGHTVVLRKMKQLQDLGHQIVIIIGDFTGKIGDPTGKSKARKALTTEQVL
                     ANAKTYEEQIFKVLDKEKTIVRFNSEWLAKLNFEDVIKLAATITVARMLEREDFKKRY
                     EGQMPISVHEFFYPLMQAYDSIALEADIELGGTDQRFNLLMGRSLQREFGMESQIVIM
                     MPLIEGLDGKEKMSKSLGNYIGIDEEAGIMYQKSMEIPDELIIKYYNLVTDVHPDEVN
                     KIESQLKEGSVNPRDIKMNLAREIVTLYHGEESAKEAEERFKSVFQKGQIPEDIQTIQ
                     VKEDGFDLIEVLVSNEIVKSKSEVRRLASQGGVKVNGEKVEDLSTIVKESELVVQIGK
                     KKFVKIELVK"
     misc_binding    complement(2364..2616)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(2768..3757)
                     /locus_tag="PC471_003250"
     CDS             complement(2768..3757)
                     /locus_tag="PC471_003250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2232 domain-containing protein"
                     /protein_id="HDF2790354.1"
                     /translation="MIYGGSVLNTTKKLTEAALLSSLFIVVTIIAVSTGFGYAIYLDF
                     IVPVFFCIICLKCDLKYTILSGITSLLIISLVLGNLGTAIWASQSVLLGIMCGYLINK
                     PTMVMDDLVYGSVFGLIVMVFIDIYASTLIGYSFMKEFQGYSKWIYFNGYTNFIYYLM
                     IALFPFGMVFCIYLLSLILGNKLHILDSNSLKKFLIFKNFRSLNQFLCCSKKAFYICV
                     SYLAIFEVLKLLNVKVDSVYLKTIFISITYISLYFIIRDSCMSLQNFIIAKFRKLLYA
                     RILFLIIILLLFFIFDVTVIGLIIINAFLNKKINIRLSQSNIVNKQINLLIEK"
     gene            3909..4349
                     /locus_tag="PC471_003251"
     CDS             3909..4349
                     /locus_tag="PC471_003251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDF2790355.1"
                     /translation="MFKIKHFNDLSLDEFYEIAKSRYEVFACEQKIFSLNDYDDIDKN
                     SYHIFLKENGLICAYARIIPKEYSSYNDVSIGRVLVLSSHRRKGLAKQMMDCAIDFIK
                     VNLHENNITLSAQTYIKNLYLSCGFKEISEVYDEAGIEHIKMRL"
     gene            4676..4903
                     /locus_tag="PC471_003252"
     CDS             4676..4903
                     /locus_tag="PC471_003252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HDF2790356.1"
                     /translation="MKNLNDIQVKKTVKVEDILSSGNLRERMLALGLTRGAVIDVVRK
                     GPKNNLTVYKIRGSKIALRQEESSLILVSDI"
     gene            4929..7049
                     /gene="feoB"
                     /locus_tag="PC471_003253"
     CDS             4929..7049
                     /gene="feoB"
                     /locus_tag="PC471_003253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HDF2790357.1"
                     /translation="MGLTHNSTKMSSLKDMFDIDNKEDQFVIALAGNPNTGKSTVFNH
                     LTGLRQHTGNWPGKTVATARGNFKYKNTEYALIDLPGTYSLFALSQEEIVARDFICFG
                     NPDAVIVVCDATCLERNLNLVFQVMELTDKVILCINLIDEARKKGITIDKKLLEDSLG
                     IPVILTAARNGSGMDELLDTLNDVSFDKYKLNNKPVRYNENIENVVKSIQPELDNIIP
                     GINSRWLGLRLIDGDESIFESMSNYIDKNSIDAINEVKKKIPDNINKQKIRDEFTKIN
                     YDYAKKLSDECCSNVAKKSTDREEKVDKILTSKIFGLPIMLLLLGTILWITIEGANYP
                     STLLSNLLLGFEPSISGILNSINCPSWLNDMLVLGLYRTLAWVISVMLPPMAIFFPLF
                     TLLEDFGYLPRVAFNLDHLFKKACAHGKQCLTMCMGFGCNAAGVIGCRIIDSPRERLI
                     AILTNNFVPCNGRFPTLIAISTIFFSSVITNSFVSSVATALCITLLIILGVIITLLVS
                     YTLSKTLLKGVPSTFTLELPPYRVPQIGRTLYTSIIDRTIFVLGRAVMVAIPAGVITW
                     IFANIYIGDLSILSHVANFLDPLAKLIGLDGFILLAFILGFPANEIVVPILLMAYLAT
                     GSMIELDSFSALGQVLREHGWTYLTALNVMLFSLLHWPCATTLLTIKKETGSLKWTAL
                     GFLMPTILAFVVCFLTTTVYNLFI"
     gene            complement(7182..8000)
                     /locus_tag="PC471_003254"
     CDS             complement(7182..8000)
                     /locus_tag="PC471_003254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDF2790358.1"
                     /translation="MEKQYFTTGEFAKLCGISKQTLIFYDKIGIFSPEYKDKNNYRYY
                     SVYQYDTLDILQSLREIGMSLEEIKEYIQNRTPQLCVKMLKEEEKKIREKIKKLRKIS
                     SKMQNRINITVEGISKMNSEEIYISKKFEEYLVLSSDLSNMSNSDFMMELIDFTNYCK
                     SKNLYQGYELGVIVSNENIKNGDYLSISSFYLKIDKKIKDKKLYVKPEGMYACIKHIG
                     KYEDSYKSYEKLKKYIYENEYKIVGNSYEESILDFFCESNEDNYMTEISIQVSR"
     gene            8207..>8442
                     /locus_tag="PC471_003255"
     CDS             8207..>8442
                     /locus_tag="PC471_003255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2790359.1"
                     /translation="MLVRVKIVIGATIRYLIKHKVNYCNKKSLDKLELKYGNIDREKA
                     VKLEIFYQYLIELEYIEMSLLIRRLDTAITSLALV"
BASE COUNT         2991 a         1214 c         1103 g         3134 t
ORIGIN      
        1 taatattatg caaataatgt catataagtt acactttagt tatcttagtt acaactttgt
       61 cggtttactt tttctacttt cttcgatata attataatat atttttgata caattcgaca
      121 aaagggggaa tgctgtaatt atggtaaaaa aaagaaaaaa gaaaactgtt acacttacta
      181 tacttgttgt aatagttatt ggtatttttt ctatggcatg ggcggacatg tctagaaaaa
      241 aagaaatgaa agaaactcaa gaaagactta aagctgaaag attagaaaaa gaaagaattg
      301 aaaaagaaaa aaatgaacct attattggtg caaaaaaaga agctgtaaaa gaatatggat
      361 atgacgcaaa aatagtttgg gacaaactta acaaatacga ctattcaaac aatggagaaa
      421 agatagtctt tcttacattt gatgatggac catctactac taatactcca caagttctag
      481 atatcttgaa aagacatggt gttagaggaa ctttctttat taagggtgat tctcttgaaa
      541 gaaaaggagc taatgaaatt ttaaaaagaa catttgacga aggaaatgca atagcccatc
      601 actcttatac tcatgactat aaaaaactat atcctaacag aagcctaaat ctcgatacat
      661 tcgtaaatga acttaacaaa actgatgaag ctatgaaaaa agtcttagga aagaattttt
      721 cctcaaatgt agttagatgc ccaggcgggt atatgtcatg gaaaaatatg gaacctctag
      781 gaaactactt aaaagagaaa aacatggcat caattgattg gaacgcttta aatgctgatg
      841 cagaaggtaa aaagaaaaat gcacaagaat tatttgaaca tgcaaagaaa tcttctgaag
      901 gcaaagaaat ggttgttctt ttaatgcatg atacttatgg aaaacaagaa acagtaaatg
      961 cattggacca aattataaca tattttaaag acaatggtta tcaatttaaa attttaatat
     1021 aataaaaaag caagtttatt gaatgtacat tttatactca atagacttgc ttttacttta
     1081 ctctatttta ctagctcgat ttttacaaat tttttctttc ctatttgaac aactaattca
     1141 ctctctttta caatagtgct taaatcctct actttttcac catttacctt tactccacct
     1201 tgagaagcta atcttctaac ttcactctta ctcttaacaa tctcattaga aactagtact
     1261 tcgattaagt caaatccatc ttccttaact tgaattgttt gtatatcttc aggtatttga
     1321 cctttttgga atacagattt aaatctttct tcagcttctt tagcagattc ttctccatgg
     1381 tataatgtta ctatttctct tgctaagttc atctttatat ctcttggatt tactgaaccc
     1441 tctttcaact gtgattctat tttattaact tcatcaggat gaacatctgt cactaaattg
     1501 tagtatttaa ttataagttc atcaggaatt tccatagatt tttggtacat tattcccgct
     1561 tcttcatcta ttccaatata gttacctaaa ctcttactca ttttttcttt accatctaat
     1621 ccttctatta aaggcatcat tattactatt tgagactcca taccaaattc tctttgtaat
     1681 gatcttccca ttagtaaatt gaatctttgg tcagttccac caagttctat atcagcttct
     1741 aatgctatag agtcatatgc ttgcattaat gggtagaaga actcatgtac tgatattggc
     1801 atttgtcctt catatctctt tttaaaatct tctctttcta acattctcgc aacagtaata
     1861 gttgctgcta attttattac atcttcaaaa tttaacttag ctaaccactc gctattaaat
     1921 cttacaatag ttttttcttt atctaaaact ttgaaaattt gttcttcata agtttttgca
     1981 tttgcaagaa cttgctctgt tgttaaagct tttctagctt tagattttcc agttgggtct
     2041 ccaatttttc ctgtaaaatc tccaattatt attactattt gatgacctaa atcttgtaat
     2101 tgtttcatct ttctaagaac tactgtatgc cctaaatgta tatctggagc acttgggtct
     2161 agacccaatt ttactatcat aggctttcct tctttttctg atttaataag cttttccctt
     2221 aactctttct catctattaa atcatctaca cctttcataa ttattcgcat ttgctcgtct
     2281 atcgatttca tttttcattc ctcctaaaat atttatataa tctataattt ttatttttat
     2341 aataaaaaaa gctttcatcc tcttaaaagg acgaaagcat tgcttacgtg ttaccacctt
     2401 agttcatcaa tatctcacga tattaatctc ttagagtaca attacatata ctcgtgtact
     2461 ataacgtgta ctaatccgat atctcttaat ggttgttatc tttcagagat agtgctctga
     2521 gatgtattca aactaaatca atttattcca tttcaccaaa ctggaactct ctgttaaata
     2581 aaatctagcc ctacttcttc tcttcatagc atttatatat tcagattatt tcatcaatta
     2641 caagaaattt ttcttaactt actcataata tattattatt tttatatttt gtcaaccttt
     2701 tttattatta tatacatgtt tttttacatt atactacttt tttatatttt ttaccaatac
     2761 ccaaaattta tttttcaatc aaaagattta tttgtttatt gactatatta ctctgactca
     2821 atcttatatt tattttctta tttaaaaatg cgtttataat tattaaacca ataactgtta
     2881 catcaaatat aaaaaacagc aataaaatta ttatcaaaaa taaaattcta gcatataata
     2941 attttctaaa cttagctatt ataaagtttt gtagtgacat acatgagtct ctaatgataa
     3001 aatagagact tatataggtt attgatataa agattgtttt taaataaaca gaatcaactt
     3061 taacattcaa cagttttaga acttcaaaaa tggctagata agaaacacat atgtaaaaag
     3121 ctttttttga gcaacataaa aattggttta aacttctaaa attcttaaat attaaaaatt
     3181 tttttagaga attagaatct aaaatatgca atttattccc taaaattaaa cttaataaat
     3241 atatgcaaaa caccatacca aaaggaaaca atgctatcat taaataataa ataaaatttg
     3301 tatacccatt aaaataaatc catttgctat atccttgaaa ttctttcata aaactataac
     3361 ctattaatgt tgaagcatat atatcaataa aaaccattac aataagccca aatactgaac
     3421 catatactaa atcatccata accattgtag gcttatttat tagatagcca cacattatac
     3481 caagtaatac actctgactt gcccaaatag cagttcctag atttccaagt accagagaaa
     3541 ttattaaaag cgaagtaatt ccagataaaa tggtatactt taaatcacat ttcaagcaga
     3601 ttatacaaaa aaatactggc actataaaat ctaagtaaat agcatatcca aatccagtac
     3661 tcacagctat aattgttact actatgaata aagatgacaa taaagctgct tcagttaatt
     3721 ttttagtagt attcaaaaca ctgcctccat aaatcataaa ttttctaact aaagttttgt
     3781 ttttaatata gtatatcact ttttaaaaat atttttaata aatataaatt ttaataaagg
     3841 tatttatgtt aaaaatggta taatgtatct taatattcag ttttttaaga tgctaggagg
     3901 taattactat gtttaaaatt aaacacttta atgatttgag tttagatgaa ttttatgaaa
     3961 tagcaaaatc aagatatgag gtatttgctt gtgaacagaa gatattttca ttaaacgatt
     4021 atgatgatat agataaaaac tcttatcaca tatttttaaa agaaaatgga ttgatttgtg
     4081 cttacgctag aataattcct aaagaataca gctcttataa tgatgtttcc attggtagag
     4141 ttttagttct ttctagtcat agaagaaaag gattagcaaa acaaatgatg gattgtgcta
     4201 tagattttat aaaagttaac ttacacgaaa ataatataac cttatcagca caaacttata
     4261 taaagaacct ttacttatct tgtggtttta aagaaatctc agaagtatac gacgaagcag
     4321 gtattgaaca tattaaaatg agattatagt gttgacaagc taatattgtt aatatataat
     4381 ttaagtataa attaaatatt gaattgatat ttaggtattt gaaaatacct tacatagagt
     4441 cagaaggctt ttgctttctg acttttttat tatcaaaatt caataattat atggattcaa
     4501 agtgttaatt tttattctat attctacttt ccccaaaagt aagaattata cccaactttg
     4561 tatgataggc atcttgccta tctttttttt gtaaaagtaa caaggtaata atataatgca
     4621 tagtttaaaa taagaagatt agcccaggct aaatttcaag catggaggtg ctattatgaa
     4681 aaaccttaac gatattcaag tgaaaaaaac tgtcaaagtt gaagatatat tatctagtgg
     4741 taatttaaga gaaagaatgt tagcgcttgg tcttactaga ggagctgtta tagatgtagt
     4801 tagaaaaggt ccaaaaaata atttaactgt ctataaaatc agaggtagca agatagctct
     4861 acgacaagaa gaaagtagct taatattagt ttctgacatt taaaaatttc tagcaggagg
     4921 atattaatat ggggttgaca cataactcaa ctaaaatgag ttctttaaaa gacatgtttg
     4981 atatagacaa taaggaagac cagtttgtaa ttgcattggc tggcaatcca aatacaggta
     5041 aaagtactgt atttaatcat cttacaggac ttagacagca tactggaaac tggccaggaa
     5101 aaactgtagc tacagctcgt ggtaatttta aatataaaaa taccgaatat gcattaatag
     5161 atttaccagg aacatattct ttatttgcct tatcacaaga agaaattgta gctcgtgatt
     5221 ttatatgttt tggtaatcca gatgcagtaa tagtagtatg tgatgctaca tgtcttgaaa
     5281 gaaatttaaa tttagtattt caagtgatgg aacttacaga taaagttatt ttgtgtatta
     5341 atttaataga tgaagctaga aaaaaaggca ttactattga taaaaaactt ttagaggatt
     5401 ctcttggtat tccagttatt ttaactgctg caagaaatgg ctctggtatg gatgaactat
     5461 tggatacttt aaatgatgta tcttttgaca aatataaatt aaataataaa cctgttagat
     5521 acaatgaaaa tatagagaat gtggtaaaat caattcaacc agaattggat aatattatac
     5581 caggtataaa ttctagatgg ttgggactta gacttattga tggcgatgaa agtatatttg
     5641 aatctatgag taattatata gacaaaaatt ctatagatgc tattaatgaa gtaaagaaaa
     5701 agattccaga caatataaat aaacagaaaa taagagatga gtttactaaa atcaactatg
     5761 attatgctaa aaaactgagt gatgagtgtt gttctaatgt tgctaaaaaa tcaactgata
     5821 gagaagaaaa agtagataaa atattaacat ctaaaatatt tgggcttcca ataatgctct
     5881 tattattagg tactatattg tggattacta tagaaggagc taactatcca tccactttat
     5941 tatctaattt attactcgga tttgagccaa gtatttcggg tatattaaat agtattaatt
     6001 gtccatcatg gttaaatgat atgttggttt taggattata cagaacatta gcatgggtaa
     6061 tctctgtaat gctacctcct atggcaatat tttttccatt atttacccta cttgaggatt
     6121 ttggatattt gccaagagtt gcatttaact tagaccacct atttaaaaaa gcttgtgcac
     6181 atggaaaaca gtgtttgact atgtgtatgg gatttggatg taatgcagct ggagttattg
     6241 gttgtaggat aattgactct ccaagggaaa gacttatagc tatacttaca aacaactttg
     6301 ttccttgtaa tggtcgtttt cctactttga tagctatatc tacaatattt tttagctcag
     6361 ttataaccaa ctctttcgta tcaagcgtag caactgcact ttgcataact ttactgataa
     6421 ttctaggtgt tataataaca ttgttggttt cttacactct ttctaagacg ctattaaaag
     6481 gagttccgtc tacatttaca ttagaacttc ctccttatag agttcctcaa ataggaagaa
     6541 ctttatatac ctctataata gatagaacaa tatttgtttt aggaagggct gtaatggtag
     6601 ccatacctgc tggtgtcata acttggatat ttgcaaatat atatatagga gatttaagta
     6661 tattatcaca tgttgcaaac tttttagacc ctttagctaa attaattggt cttgatggtt
     6721 ttatattact tgcttttatt cttggattcc cagcaaatga gatagttgta cctatacttt
     6781 taatggctta cctagcaact ggttctatga tagaattaga tagctttagt gctttaggtc
     6841 aagttcttag agaacatgga tggacttatt tgacagcttt aaatgttatg ctgttcagtt
     6901 tactgcattg gccttgtgca acaacacttt taacaataaa aaaagaaact ggtagtttaa
     6961 aatggactgc cttaggattc ttaatgccta ctatacttgc ttttgttgtt tgttttttaa
     7021 caactacagt ttacaattta tttatataat tagtattaac tgacagtaga catatattag
     7081 agaaaccatt taaaaattta tatactttat tataaagcat agagatatat tcaatgataa
     7141 gtaaaattac ttaacatttg aatatatctc tattttaatt cttatctgga tacttggatt
     7201 gaaatttcag tcatataatt atcttcatta ctttcacaaa aaaagtcaag tatactctcc
     7261 tcatacgaat ttccaacaat tttatattca ttttcatata tatatttttt caatttttcg
     7321 taagatttat atgaatcctc atattttcca atatgtttta tgcaagcata cattccttct
     7381 ggctttacat atagcttttt atcctttatt tttttatcta tcttcaaata aaaacttgat
     7441 atacttaaat agtccccatt ttttatattt tcattactta ctattactcc caactcatat
     7501 ccttgataca aatttttcga tttacaataa ttggtaaaat ctattaattc catcataaaa
     7561 tcactattac tcatattact caaatcgctt gatagaacta aatattcttc aaactttttg
     7621 gatatataaa tttcttcact attcatttta gaaattcctt caactgttat atttattcta
     7681 ttttgcatct tagaacttat ctttcttagt ttttttattt tttctcttat ctttttttct
     7741 tcttctttaa gcattttcac acaaagctgg ggagttctat tttgtatata ctcttttatt
     7801 tcctctaaac tcattcctat ttctcttaat gattgtaata tatcaagtgt atcatattga
     7861 taaactgagt aatatctata attattttta tctttatatt caggagaaaa aatacctatt
     7921 ttgtcataga aaattaatgt ctgcttagat ataccacaca atttagcaaa ttcacctgtt
     7981 gtaaaatact gtttttccaa gggttataca ctccttttct ttttgttgtc tatatagtta
     8041 ctatattctt tattatttac taaatcaagt agttattatt ttagaaaatc aatatggttt
     8101 tgattttaaa gcttaaatat cagttagatg tcagttataa atattttacc ctttcccatc
     8161 ataaaaaaga tatcccctct taacttttgg atggaatatt atatttatac ttgttagagt
     8221 gaaaattgta ataggtgcta caataagata tttaattaaa cataaagtta attattgtaa
     8281 taaaaaatct ttagataaat tagagttaaa gtacggaaat atagatagag aaaaagctgt
     8341 taaactagaa atattttatc agtatttaat agaacttgaa tatatagaaa tgagtttatt
     8401 aataagaaga ttagatactg caataacatc attagcatta gt
//
LOCUS       DAMJYR010000147         8389 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.152, whole genome shotgun sequence.
ACCESSION   DAMJYR010000147 DAMJYR010000000
VERSION     DAMJYR010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8389
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.152"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            57..212
                     /locus_tag="PC471_003256"
     CDS             57..212
                     /locus_tag="PC471_003256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2790360.1"
                     /translation="MKGILKELTAKLYSFFYFMFWGFYFSAGYFFYKNICTSFLVRPY
                     FSDFEHK"
     gene            342..1355
                     /gene="aroF"
                     /locus_tag="PC471_003257"
     CDS             342..1355
                     /gene="aroF"
                     /locus_tag="PC471_003257"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003452924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HDF2790361.1"
                     /translation="MIVVLKMGADKNEVKKLIEAIGREGVEVNPIDGTELTVLGLVGD
                     TSKIDAKRIEANKVVEKVMHVVEPFKKANRKFHPEPSIINVNGMEIGSKKIAMIAGPC
                     SVETEDQIVSIAKDVKKSGAGFLRGGAFKPRTSPYAFQGLKYDGLDLLKKAKEKTGLP
                     IVTEIMSTQDIDIFEENVDVIQVGARNMQNFDLLKELGKTNKTILLKRGLSATIEEWL
                     MSAEYIMAGGNENVVLCERGIRTFETYTRNTLDLSAILAVKKLSHLPVIVDPSHAAGK
                     SWMVDSLSKAAIAVGADGLIIEVHNDPAHALCDGKQSIKPNEYDELISELKTIASAVG
                     REI"
     gene            1377..2441
                     /locus_tag="PC471_003258"
     CDS             1377..2441
                     /locus_tag="PC471_003258"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HDF2790362.1"
                     /translation="MEEKLLVNLKENSYNIFIKKNILKNIGEEIKKIYFGNKIFIVTD
                     ENIAKLYLDKVKNNLSESGFEIKSLILKSGEETKSFNTLPKIYDSLLDFKLTRKDLII
                     TLGGGVIGDLGGFAAATFLRGISFIQIPTSLLAQVDSSVGGKVGVDLERGKNLVGSFY
                     QPKAVFIDPNVLLSLPEKFYRDGMAEVIKYGCIKDKTFFNKLLSLKNREEVMQHIEYI
                     IHKCCFIKKTVVEADEKDFGDRMLLNFGHTLGHAIEKYYNFTGYTHGEAVAIGMYKIT
                     LESEKIGITKKGTSELIKSILMNYNLPYEIELRDNKSIVDAISLDKKNLGDTLKIILL
                     KDIGESIIYNTTSKFFQEVR"
     gene            2441..3754
                     /gene="aroA"
                     /locus_tag="PC471_003259"
     CDS             2441..3754
                     /gene="aroA"
                     /locus_tag="PC471_003259"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HDF2790363.1"
                     /translation="MGSLKIYPSKLSGDVKIPPSKSMAHRAVICSSLSNGKSRISNID
                     FSDDIIATIRAMTSLGAIIEKKEDILEISGIFSKEGILNRENQLNQPKLTIDCNESGS
                     TLRFLVPISLTFDGVKRFIGRGNLGKRPLDTYYEIFDRQNIKYSYKENQLDLIISGKL
                     KPDEFRVKGNISSQFITGLLFILPTLESDSKIIITTELESKGYLDLTLSTIKDFGVEI
                     INNNYKEFIIKGNQTYKARDYKVEGDYSQGAFYLSADAIGEDISILDLKEDSLQGDSE
                     VVEILSRMGMEILREGNKIKGITNGLNGTLIDASQCPDIIPVLSVVASLSIGRTTIIN
                     AGRLRIKECDRLHAINVELSKLGANIEEKEDSLIIEGVSKLNGGVEVWSHKDHRIAMT
                     LAIASCRCDKPIILKDFECVSKSYPHFFKDFKMLGGRIDEWNMGK"
     gene            3732..4811
                     /gene="aroC"
                     /locus_tag="PC471_003260"
     CDS             3732..4811
                     /gene="aroC"
                     /locus_tag="PC471_003260"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HDF2790364.1"
                     /translation="MSGIWGNNLKVSIFGESHGNAIGINIDGLPSGIELDLDNIDKEM
                     KRRAPGKNSISTSRNESDIPEILSGYFNGRTTGTPLCAIIRNSDTRSKDYGELKNLMR
                     PGHADFTGNVRYSGFNDYRGGGHFSGRITAPLVFCGAICKQILSQKGIEIGAHIKKIK
                     NIEDMSFDYVNISKQQLSNLQTLELPLLDLSKEEAMKNTIIDAKNQGDSVGGIIECAV
                     VGINVGLGNPFFDSVESTLSHLLFSVPAVKGVEFGLGFELADMYGSQSNDEMYYEGNQ
                     VKSKTNNNGGIIGGITTGMPIIFKVAIKPTPSISRQQNTVNIKDKKDDILYIKGRHDP
                     CIVQRAIPVIEAVTAIGIFDLMKGR"
     gene            4814..6010
                     /gene="pheA"
                     /locus_tag="PC471_003261"
     CDS             4814..6010
                     /gene="pheA"
                     /locus_tag="PC471_003261"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HDF2790365.1"
                     /translation="MEDLTRYRNEIDNIDKELIQLFEKRMNTVLEIARHKMKNNTTIL
                     QKDREEKVLSRAVDNLDNKAYSQETVQFFNSIMEISRGLQKRLIDNDGEQKIEFSLDS
                     SNKKFDLSNVNRYKSLKDELNKKNILVGFPGKSGSFTEEALNKFFNKKISKKQFKEFE
                     DVFIALKNKEINYGIIPIENSSTGAISETYDLLRKYGFYIVGEECIKIDQNLIGIKDT
                     KLEDIKEIYSHPQGIGQCSKFLKQNSTWKLIPFNNTATSAELVRKLQDKTKAAIASKK
                     AASIYGLEIISPCINDIANNYTRFVVISNQIHIEEESNKMSVVFSVEHEAGKLYKVLG
                     YFAKNNINMTKIESRPMKNASWRYFFYIDFECSIYNAKVYDLLELIEHNTAYFKFMGV
                     YKNKTE"
     gene            6057..6866
                     /gene="aroE"
                     /locus_tag="PC471_003262"
     CDS             6057..6866
                     /gene="aroE"
                     /locus_tag="PC471_003262"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HDF2790366.1"
                     /translation="MNFFGLVGEKLSHSVSPQIHKRVFEILNIESAYKNFEISKEDIS
                     KLDGAIKLLGIQGVNVTVPYKERIMKYLDFISPEAKRIGAVNTILLRENMLYGYNTDY
                     FGLDSMFKMANIDVQGKVAVILGTGGASKAALTYFIDSGIEKLYVSTRKKDDKKLLNS
                     KAILIDYEELKHIKGDIILNATPVGMYPNVGISPVSKSIIQNFDILIDLIYNPGETEF
                     LRIGNSMGKKTCDGLYMLVGQAIKSQEIWQDTKIDNSILDVIYNELKLEFL"
     gene            6878..7411
                     /locus_tag="PC471_003263"
     CDS             6878..7411
                     /locus_tag="PC471_003263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HDF2790367.1"
                     /translation="MINKTKEKLILIGMPGSGKTTIGKLLAKEYNCSFCDMDDYIIQI
                     SQKSIAELFSEGEDIFRNYETQACRELSISDKTVISTGGGVIKKDVNMEILKETGIII
                     FIDRPIQKILEDININSRPLLKNGKDRLYNLYNERINLYKKFSDIEILNDKSLNNAVY
                     NITNAVSENFKFDFKEK"
     gene            7432..8271
                     /locus_tag="PC471_003264"
     CDS             7432..8271
                     /locus_tag="PC471_003264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HDF2790368.1"
                     /translation="MNIVIVGLGVIGGSFAKALKKAGYENVFGVDVDLETLKKAEKAK
                     IIKKGCTTGKELFKKADLIILSIYPRLVVDFLNNNKNFFKKGTIITDTTGIKETLIND
                     VLQIIPDDIDFIFGHPMAGREKKGIDFASEQVFNGANYIITPTGRNNIKNLELVENLI
                     LEIGFKRVKKLTSQKHDEIIAFTSQLPHVMAVALINSDEEGRDTGKFIGDSYRDLTRI
                     ANMNEDLWSELFLGNRDNLLKVIENFESEVNLVKEAIFNNDKNKLIEYFKKSSIRREA
                     LEK"
BASE COUNT         3444 a          831 c         1513 g         2601 t
ORIGIN      
        1 ttgttataat tattaaacaa gaagaataat aaattataaa aatggaggat aatactatga
       61 aaggaatatt aaaagaacta acagcaaaac tttatagctt tttttacttt atgttctggg
      121 gcttttattt tagcgctggc tatttctttt acaaaaatat atgtacttcc tttctggtga
      181 ggccttattt tagtgatttt gagcataagt aaattttata aaaatgatta ttttatatat
      241 ttatataaga tttagtgctt gatagattat tttagagaac tcattagtga gttctctttt
      301 ttattttaaa tataaaatta taatattggg aggtagtaaa aatgatagta gtattaaaaa
      361 tgggagcaga taaaaatgaa gttaaaaaat taattgaggc tattgggaga gaaggagtag
      421 aagttaatcc catagatgga acagaattaa ctgttttagg tttagttgga gatactagta
      481 aaatagatgc caaaagaata gaagcaaata aggtagttga aaaagttatg catgtggtag
      541 agccttttaa aaaggccaat agaaaatttc atccagaacc ttcaatcata aatgttaatg
      601 gaatggaaat tggttcaaag aaaattgcta tgatagctgg gccttgttct gttgaaacag
      661 aagaccaaat agtatctata gctaaagatg tgaaaaaatc aggtgcagga tttttgagag
      721 gtggagcatt caaaccaaga acatcaccat acgctttcca aggattgaaa tatgatggat
      781 tggatttatt aaaaaaagca aaagaaaaaa cagggcttcc tatagttaca gaaataatgt
      841 caacacaaga tatagatata tttgaagaaa atgtagatgt aattcaagtt ggagctagaa
      901 acatgcaaaa ctttgattta ttaaaagaat taggtaaaac aaataaaaca atacttttaa
      961 aaagaggatt atctgctaca attgaagagt ggctaatgtc agcagagtat ataatggctg
     1021 gaggaaatga aaatgtagtt ctttgtgaaa gaggtataag aacttttgaa acatatacta
     1081 gaaatacttt ggatttaagt gcaattttag ctgtgaagaa acttagtcat ttaccagtta
     1141 tagttgaccc aagtcatgca gcaggtaaat catggatggt tgactcttta tctaaggcag
     1201 caattgcagt tggagcagat ggtctaataa tagaagttca taatgaccca gcacatgcac
     1261 tttgtgatgg aaaacaatcg attaagccaa atgaatatga tgaattgata agtgaattaa
     1321 agactatcgc atcagcagtt ggtagagaaa tataatctat aaaaggatgg ttaaacgtgg
     1381 aagaaaaact attagtaaat ttaaaagaaa atagctacaa tatatttatt aaaaaaaata
     1441 tactaaagaa tataggagaa gaaataaaaa aaatatattt tggaaataag atttttattg
     1501 taactgatga aaatatagca aaattatact tggataaagt aaaaaataat ttaagtgaat
     1561 ctggatttga aattaaatct ttaatattaa aatctggtga agaaacaaaa tcatttaata
     1621 ctcttcctaa aatatacgat agtttacttg actttaagtt aactagaaaa gatttgatta
     1681 ttactttagg aggaggggta ataggtgatt taggtggatt tgcagcagca acttttttaa
     1741 gaggtataag ttttattcaa attccaacat ctttattagc acaagttgat agttctgtag
     1801 gtggaaaagt tggagttgac cttgagagag gcaaaaactt agtgggaagc ttttaccaac
     1861 caaaggctgt gtttatcgac ccaaatgtat tattatcact tccagaaaaa ttctatagag
     1921 atggaatggc agaagttata aaatatggat gtataaaaga caaaactttt ttcaataaac
     1981 ttttaagttt aaaaaataga gaagaagtaa tgcaacatat agaatacatt atacataaat
     2041 gttgctttat taaaaaaact gtagttgaag ctgatgaaaa agactttgga gatagaatgc
     2101 ttctaaactt tggccataca ttaggtcatg ctattgagaa atactacaat tttacaggtt
     2161 atacccatgg agaagctgtt gccataggaa tgtataagat aactttagaa agtgaaaaga
     2221 taggaattac aaaaaaaggt acatcagaac ttataaaatc aattttaatg aattataatt
     2281 tgccttatga gattgaatta agagataata aatctatagt agatgctata tctcttgata
     2341 aaaaaaatct aggtgataca ttaaaaataa tacttttaaa agatattgga gagagtatta
     2401 tatataatac tacttctaaa ttttttcagg aggtaagata atgggaagtc taaaaatata
     2461 tccaagtaaa ttatctggag atgtaaaaat accaccatca aaaagtatgg cacatagggc
     2521 agttatttgt tcatctctaa gcaatggaaa aagtagaatt tctaatatag atttttctga
     2581 tgatataata gctactataa gagcaatgac atcattagga gctattatag aaaagaaaga
     2641 agatatttta gaaatttcgg gaattttttc taaagaaggt attttaaata gagagaatca
     2701 gttgaatcaa cctaaattga caatagattg taatgaatca ggctctacat tgagattttt
     2761 agtacctata tctttaacat ttgatggtgt aaaaaggttt ataggtagag gtaatcttgg
     2821 aaaaagacca cttgatacct attatgagat atttgacaga cagaatataa aatatagtta
     2881 caaagaaaat cagttagatt tgattatatc aggtaagctt aaaccagatg aatttagagt
     2941 aaaaggaaat ataagttctc agtttatcac aggtttactg tttatattgc ctacgctaga
     3001 aagtgattca aagataataa ttactactga gctagaatct aaaggatatt tagatttaac
     3061 tctttcaacg ataaaagatt ttggggtaga aattataaac aataactata aagaatttat
     3121 tataaaagga aatcaaacgt ataaagcgag agattataaa gtagaaggag attattctca
     3181 aggagcattt tatttatcag cagatgctat aggagaagat attagcatat tagaccttaa
     3241 agaagattct cttcaagggg atagtgaagt agttgaaatt ttatctagga tgggaatgga
     3301 aatattaaga gaaggcaaca aaataaaagg aataacaaat ggtttaaatg gtactttgat
     3361 agatgcatct caatgcccag atataatacc tgtattatct gtagtagcat ccttaagtat
     3421 agggaggact acaataatta atgctggtag gcttagaatt aaagaatgtg atagacttca
     3481 tgcaataaat gttgagttaa gtaaacttgg agcaaatata gaggagaaag aggatagttt
     3541 gataatcgaa ggagtaagca agttaaatgg tggagtagag gtgtggagtc acaaagacca
     3601 tagaattgct atgacacttg caattgcttc ttgtagatgt gataaaccaa taatattaaa
     3661 agattttgag tgtgtatcaa aatcttatcc acattttttt aaagatttta agatgttagg
     3721 aggtcgaatt gatgagtgga atatggggaa ataatttaaa agtatcaatt tttggagaat
     3781 ctcatggaaa tgctataggt ataaatatag atggtcttcc ttcggggatt gaacttgacc
     3841 ttgataatat tgataaagaa atgaagagaa gagcacctgg aaaaaattct atatctactt
     3901 caagaaatga aagtgatata ccagaaattc taagtggata ttttaatgga agaactacag
     3961 gaacgccatt atgtgcaatc ataagaaata gtgatactcg ttcaaaagat tatggagagc
     4021 ttaaaaattt aatgagacca ggacatgcag atttcacagg taatgtgaga tattctggat
     4081 ttaatgacta tagaggggga ggtcattttt caggtaggat aacagctcca ttagtatttt
     4141 gtggggctat atgtaaacaa attttatcac aaaaaggaat agagatagga gcacacataa
     4201 aaaaaattaa aaatatagaa gatatgagtt ttgattatgt aaatatttca aaacaacagt
     4261 tatcaaattt gcaaactttg gaactacctt tacttgattt atcaaaggaa gaagccatga
     4321 aaaatacgat tatagatgca aaaaatcaag gagattcagt tggtggtata attgagtgtg
     4381 cagtagtagg tattaatgta gggcttggaa atccattttt tgactctgtt gaatctactt
     4441 tatcacattt attgttttct gttcctgctg taaaaggtgt agagtttggt ttaggttttg
     4501 aacttgcaga tatgtatggg tctcaaagta atgatgaaat gtattatgaa ggaaatcaag
     4561 ttaagagcaa aacaaataac aatggaggaa ttataggagg gattactact ggaatgccaa
     4621 taattttcaa agtggcaatt aagccaactc catcaattag cagacaacaa aacacagtta
     4681 atataaaaga taaaaaggac gatattttat atattaaagg tagacatgac ccttgtatag
     4741 ttcaaagggc tatacctgta atagaagcag taacagccat tggaattttt gatttgatga
     4801 aaggacgata ataatggagg atttaacgag atatagaaat gagattgaca atatagataa
     4861 agagcttatt caattatttg aaaagagaat gaatacagtt cttgaaattg ccagacataa
     4921 aatgaaaaat aacactacta tacttcaaaa agatagagaa gaaaaagtac taagtagagc
     4981 tgtagataat ttagataata aagcatattc acaagaaacg gtacagtttt ttaattctat
     5041 aatggaaatt agtagaggat tacaaaaaag attgatagat aatgatggtg agcagaaaat
     5101 cgagtttagt ttagattcat caaataagaa atttgatttg tcaaatgtaa atagatataa
     5161 gtctttaaaa gatgaattaa ataaaaaaaa tatcctagta ggatttccag gaaaatcagg
     5221 ttcatttaca gaagaagcac taaataaatt cttcaacaaa aaaatatcaa aaaaacaatt
     5281 taaagaattt gaagatgttt tcatagcact taaaaataaa gaaataaact atggaataat
     5341 ccccatagaa aattcatcta caggagctat ctctgaaact tatgaccttt tgagaaagta
     5401 cggtttttat atagttggag aagaatgtat aaaaatagac caaaatttaa tagggataaa
     5461 agacacaaag cttgaagata taaaagaaat atattctcat ccacaaggta taggacaatg
     5521 tagtaaattt ttaaaacaaa atagtacatg gaaattgata ccatttaata acacagcaac
     5581 aagtgcagaa cttgtaagaa aacttcaaga taaaacaaaa gcagcaattg caagcaaaaa
     5641 agctgcaagt atttatggat tagaaataat aagtccttgt ataaatgata ttgcaaacaa
     5701 ctatacaaga tttgtagtaa tcagtaatca aatacacatt gaagaagaat caaacaaaat
     5761 gagtgtagta ttttctgttg aacatgaagc aggaaaactt tataaagtac ttggatactt
     5821 tgctaaaaat aatataaata tgacaaaaat agaatcaaga cctatgaaaa atgcctcatg
     5881 gagatatttc ttttatatag attttgagtg ttctatatac aatgctaaag tgtacgattt
     5941 attagaattg atagaacata atacagcata ttttaaattt atgggagtat ataaaaataa
     6001 aactgaatag ataagataaa tttttattta atttataatt attaggaggt gtaagtatga
     6061 attttttcgg tctagtaggt gaaaaactct ctcacagtgt ttcacctcaa atccacaaga
     6121 gagtattcga gatattgaat attgaaagtg cttataaaaa ctttgaaatt tcaaaagaag
     6181 atatatctaa gctagacggt gctataaaat tattaggaat acaaggggta aatgttactg
     6241 taccatataa ggagcgtatt atgaaatatc ttgattttat atcaccagag gcaaaacgta
     6301 taggagcagt taatacaatt ttacttagag aaaatatgtt atatggttac aatacagatt
     6361 actttggtct tgatagtatg tttaaaatgg ctaatattga tgttcaagga aaagtagcag
     6421 ttatattagg aactggaggt gcttctaaag ctgctcttac atattttatt gattctggta
     6481 tagaaaagct ttatgtttca actagaaaaa aggatgataa aaaattatta aatagtaaag
     6541 ctatattaat agattatgaa gaacttaaac atataaaagg agatataata ttaaatgcta
     6601 caccagtagg tatgtaccct aatgtaggaa tttcaccagt gtcaaaatct attatacaaa
     6661 attttgatat attaatagat ttaatttaca atcctggaga aacagagttt ttaagaattg
     6721 gtaatagtat gggtaaaaaa acttgtgatg gattatatat gctggtgggg caagcaataa
     6781 aatctcagga aatttggcaa gatactaaaa tagataatag tattttagat gttatatata
     6841 atgaactaaa attagaattt ttgtaaggga gattgttatg ataaataaaa caaaagaaaa
     6901 attgatatta ataggtatgc caggtagtgg aaaaactaca attggtaaac ttttggccaa
     6961 agaatataat tgtagtttct gtgatatgga tgattatata attcaaatat ctcaaaaaag
     7021 tatagcagag ttgttttcag aaggagaaga tatctttagg aactatgaga ctcaagcatg
     7081 tagagaactt tctataagtg ataaaacagt gatatctaca ggtggaggtg taataaaaaa
     7141 ggatgttaat atggaaatac taaaagaaac aggtataata atttttattg atagacctat
     7201 acaaaagatt ttggaagata taaatattaa ttcaagacca ttattaaaaa acggaaaaga
     7261 tagattgtat aatctttata atgagagaat aaatctttat aaaaaatttt cagatataga
     7321 gattttaaat gataaatcat taaataatgc tgtatataat attacaaatg cagtcagtga
     7381 aaattttaaa tttgacttta aagaaaaata atctaggggg aaataaaaat aatgaatatt
     7441 gttattgtag gactaggagt tataggaggc tcttttgcta aagcattaaa aaaagctgga
     7501 tatgaaaatg tatttggagt agatgtagat ttagaaactt taaaaaaagc agaaaaagca
     7561 aaaatcataa aaaaaggatg tactacaggt aaagagttat ttaaaaaagc tgatttaatt
     7621 atattatcta tatatcctag attagttgta gattttctaa ataataataa aaactttttt
     7681 aaaaaaggga ctataattac tgatactaca ggaataaaag aaacacttat caatgatgtg
     7741 ttgcaaatta ttccagatga tatagatttt atatttggac atccaatggc aggtagggaa
     7801 aaaaagggta ttgattttgc aagtgaacaa gtttttaatg gcgcaaatta tataattact
     7861 ccaactggca gaaacaacat aaaaaattta gagttagttg aaaatttaat tttagagatt
     7921 ggatttaaaa gagtaaaaaa gttaacttca caaaaacatg atgagattat agcttttact
     7981 tctcagttac ctcatgttat ggcagttgct cttataaata gtgatgaaga aggtagagat
     8041 acgggtaaat ttattggaga tagttataga gatttaacta gaattgcaaa tatgaatgaa
     8101 gacttatgga gtgaactttt tttaggaaat agagataatt tactaaaagt tatagaaaac
     8161 tttgaatcag aagtaaattt agtaaaagaa gctatattta acaatgataa aaataaatta
     8221 atagagtatt ttaaaaaatc atcaataaga agagaagctt tagaaaaata aatattaata
     8281 tttaagctat atatagatgg gtataaatat aagcgatagt gtatttaatt gtattaataa
     8341 atatggattc aatataatta aatatgctat tgatatattt gtaccctta
//
LOCUS       DAMJYR010000148         8321 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.153, whole genome shotgun sequence.
ACCESSION   DAMJYR010000148 DAMJYR010000000
VERSION     DAMJYR010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8321
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.153"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(119..988)
                     /locus_tag="PC471_003265"
     CDS             complement(119..988)
                     /locus_tag="PC471_003265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HDF2790369.1"
                     /translation="MEYDMPLYRPPSEAYSLIIQVTLGCSHNKCTFCSMYKSKKFKIK
                     PLEVIKNEIDIFRRYYKHVERIFLADGDALIIPMEKLRDIILYIKEVFPECERITMYG
                     SPKSIEKKSDDELKELRKLGVKMIYLGLESGSNEVLEDIKKGFSSEQLIKVGRKVKKS
                     GIKLSATVIAGLGGTKKTHQHAVDTGKMLGAISPDYVGVLSLMVEPNTELYDLLKAGE
                     FTILEDKAVLQEIKEMIKNIDANEKIVFRSNHASNYANLKGVLPDDKQRLIDEIDYYL
                     SNQKLKREEYRRL"
     gene            complement(1085..1942)
                     /locus_tag="PC471_003266"
     CDS             complement(1085..1942)
                     /locus_tag="PC471_003266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDF2790370.1"
                     /translation="MSKDVKVKRNKKYDTFSITMELERPLLYDEVENKDDKNNNRKFN
                     IKNKIFKGIGILFVLILLGFFIWINKTYEPQKLAKEALVSNSKVEVTVNENISFTPKG
                     RKVSKGLILYPGAKVEIESYAPLARKIAESGYEVVIVKMPLNLAILGTNKAQKVMDSY
                     NNIEHWVIGGHSLGGVAASNFARDNKLIEGVVFLASYPMGDELKELGKKVISIWGSKD
                     GVVNFKSLVESKQKLPDDTTYVEIEGGNHAQFGDYGKQKGDNDAIISQEKQLNITANS
                     IIKFLKNIS"
     gene            complement(2157..4175)
                     /locus_tag="PC471_003267"
     CDS             complement(2157..4175)
                     /locus_tag="PC471_003267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HDF2790371.1"
                     /translation="MTKKLVISKTKLFIFIIAFISIFIYLFGSKNTLIGVGIVTAMLT
                     LLERDLTISPIKNLLKYLAINIILGILSFFAVQNMYLGVLLNFIALFIIGYVFSYDLK
                     RAVYVPFGLMYIFMVSIPVGKSEFPMRLSALAVGAVIIMIAQFVMNRNRMKNVGDKEL
                     ISICDELLEKISLLKNTINDDSSIIKSSMRKIDSCNYRINSISKNLKMVIFDNRKDDF
                     YISIRGIDIMNILFSLERISLILERYKKDSKEFEDEDIKNILEESSINSKSDVLVVAT
                     KEINYIKMCLENKDTITDKEILGFRDYVIAQDTKNINLKEIYSVLENLYEFLVDYKKV
                     KSRDEKKAERKSKIPHEFKRLSIYKKNFNLNSIRFSYAVKIALATAVAGFIMDYFHLR
                     DGRWIMLTVFSLTQPYAENCIQRSRKRIEGTFIGAVIFIVLFSIIKDSTLRSLIVLLA
                     GYINSYVVDYRKLMVCVTVSALGSAVVMGDPNVLTISRISYVALGAIIALIVNKFILP
                     YDAKTGYQHVIEMYKGIVKNIIDEVNKSIENVADVYYIKNLLLIPSLIEDRLMLINAI
                     YKDKHQEDFLENQKLLISNMYNLYINVNKNKIKDEDVEKILRDTNYISNYNVDKYDEG
                     RSVILESIINTKSLGDKIICLNLLQTLNGVKEMYRISNITKVSIKEAA"
     gene            complement(4446..5222)
                     /locus_tag="PC471_003268"
     CDS             complement(4446..5222)
                     /locus_tag="PC471_003268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HDF2790372.1"
                     /translation="MHKETLDKLTNAAINKINLLNTSKVKYLVSSAFAGLYVGIGILL
                     IFTIGGLLTDAGSPMTKIVMGLSFAIALSLVIMTGTELFTGNNMVMSAGMLNKGVSIK
                     DTSKIWAYSWVGNLIGALVLGIIFVGTGLIDKGPVAEFFANTAASKASMPFTALFFRG
                     ILCNILVCVSVLCSFRTNSDTAKIIMIFLCLFAFITSGFEHSVANMTIYSVSLFSPTI
                     NTVTIGGAIYNLVAVTLGNIVGGALFMGLGTYILGKEKLN"
     gene            complement(5279..6250)
                     /gene="asrC"
                     /locus_tag="PC471_003269"
     CDS             complement(5279..6250)
                     /gene="asrC"
                     /locus_tag="PC471_003269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite reductase subunit C"
                     /protein_id="HDF2790373.1"
                     /translation="MIRDLNTKKVMKNAYRITKTKYETSLRVRVPGGLIDPESLAVVS
                     KIASEYGNGQVHITTRQGFEILGINMEDMEEINKIIQPVIEKMNINQSEKNAGYPAAG
                     TRNIAACIGNKVCPKAQYNTTEFAKRIEKAIFPNDLHFKVALTGCPNDCIKARMNDFG
                     IIGMALPIYEKDRCVNCGACVKKCSKISVGALKTENNKVVRDKDKCIGCGECVLNCPT
                     NAWTRDEKKYYRLAIMGRTGKKNPRLAEDFLLWVDEDSIIKIILNTYRYVEKYIDKDA
                     PGGKEHIGYIIDRTGFMEFKKWALEGVELPEITKMYENIYWSGIKYL"
     gene            complement(6263..7054)
                     /gene="asrB"
                     /locus_tag="PC471_003270"
     CDS             complement(6263..7054)
                     /gene="asrB"
                     /locus_tag="PC471_003270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfite reductase subunit AsrB"
                     /protein_id="HDF2790374.1"
                     /translation="MNPYIPVSAKILDIVKHTDKEWTFRVNCNTKGVLPGKFYEISIP
                     KYGESPISVSGYGEDYIDFTIRNVGKVTSELFNYKEGDSFFIRGPYGNGFDVSLYEGR
                     EIVVVAGGSALAPVRGIVEYFYNNKEKCEKFKLIVGFKSPKDILFADDLKRWSKKLDI
                     LVTVDGAEEGYSGNVGLVTKYIPELDMKDINNTSIVVVGPPMMMKFTVEEFLKRDLDE
                     KNIWVSYERKMCCGIGKCGHCKMDDTYICLDGPVFDYSYAKNLID"
     gene            complement(7055..8062)
                     /gene="asrA"
                     /locus_tag="PC471_003271"
     CDS             complement(7055..8062)
                     /gene="asrA"
                     /locus_tag="PC471_003271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfite reductase subunit AsrA"
                     /protein_id="HDF2790375.1"
                     /translation="MKLKLDSSKFNEGLNYLKKDYKIFAPVLIPFKGTFSDTDVIRYK
                     EVNAFEEMEFAKKANFSPKEVVLPINQVLFYFTEKEFKESDLDNKKILIFLRACDING
                     IKRLDEIYLNNGEEKDYFYKNIRDKVKFALVGCKESFRNCFCVSMDSNKTDNYSIGLN
                     IEGDTLYLDIKDSEFEVFNGEATEFNVDYVTDNLISVDVPENIDSNEMIGNSIWDEYD
                     TRCIGCGRCNFVCPTCSCFTMQDIFYKENENVGERRRVWASCQVDGYTDIAGGNSFRK
                     KQGERMRFKTMHKIHDFKKRFGYHMCVGCGRCDDACPQYISFSKCIEKINDLVISKEE
                     V"
BASE COUNT         2674 a         1507 c          756 g         3384 t
ORIGIN      
        1 ttgtttttta ataaaaaatc atgctacatc aatagtaagc aactattaat gtagcatgat
       61 tttttgtatc taaataatct tagtatttac ctatcagtat agattgatat atcaaaaact
      121 atagtcttct atattcttct ctttttagct tttggttact tagatagtaa tcaatttcat
      181 caattagacg ttgtttatca tcaggtaata caccttttaa attagcataa tttgatgcat
      241 gattacttct aaatacaatt ttttcattag catctatatt ctttatcatc tctttaattt
      301 cttgtaacac agccttatcc tctagtattg taaactctcc tgccttaagt aaatcgtaaa
      361 gttcagtatt aggttctacc attaaactaa gtacacccac ataatctggt gaaattgctc
      421 ctagcatttt tcctgtatca actgcatgct gatgtgtttt tttagttcct ccaagacctg
      481 ctattacagt ggctgataat ttaatcccac tttttttaac ttttctacca acttttatta
      541 attgttcact actaaaacct ttcttaatat cttctaatac ttcattgctt ccactttcaa
      601 gccctagata aatcatttta acaccaagtt ttcttaattc tttaagttca tcatcagatt
      661 ttttctctat agatttagga gagccgtaca tagtaattct ttcacactct ggaaaaactt
      721 cttttatata taaaataata tctcttaatt tctccattgg tattatcaat gcatctccat
      781 ctgctaagaa tattctttct acatgcttat aatatcttct aaatatatca atttcatttt
      841 ttattacttc tagtggcttt attttaaatt ttttagattt gtacatacta caaaacgtac
      901 atttgttatg agaacatcct agtgtgactt gtattataag actataagcc tcacttggtg
      961 gtctgtaaag tggcatatcg tattccatat taaaatcccc cttgtaataa aatattcatt
     1021 ctattatatt attaatattt actttaaata ataacttata aaattatatt ttttaaattt
     1081 tagtttaaga tatattcttt aaaaatttaa tgatactatt ggcagtaata ttaagttgtt
     1141 tttcttgact tataatagca tcattatctc ccttttgttt tccataatct ccaaattgtg
     1201 catgatttcc tccttcaatc tcaacataag ttgtatcatc aggaagtttt tgttttgatt
     1261 ctacaagact tttaaaattg actactccat ctttgctccc ccatatagat ataacttttt
     1321 ttcctaattc tttcaactca tcacccattg gataagaagc taaaaataca acgccttcaa
     1381 ttaatttatt atcacgtgca aaatttgatg ctgcaactcc tcctaatgaa tgacctccta
     1441 ttacccaatg ttctatgttg ttgtaagaat ccataacttt ctgtgcctta ttagttccaa
     1501 gtatagctag attaagtggc atctttacga ttacaacttc atatccactt tctgctattt
     1561 ttcttgcaag tggagcataa gattctattt ctactttagc tcctggatac aaaattaaac
     1621 ctttagaaac ttttcttcct tttggtgtaa atgaaatgtt ttcatttaca gttacttcaa
     1681 ctttactatt gctgactaga gcttcttttg ccagcttttg aggttcatat gttttattta
     1741 tccatatgaa aaagccaagc aaaatcaata caaatagtat acctattccc ttaaatatct
     1801 tattctttat gttaaatttt ctattattat ttttatcatc tttgttctca acctcatcat
     1861 aaagtaaagg tctctcaagt tccatagtta tactaaatgt atcatatttc ttattacgct
     1921 ttacctttac atctttactc ataaatctaa cccccataat atttgaatac tattatagtt
     1981 tatcctaagt aaattgtttt ttcaaccaat gaaaaaaatc aatattttaa ctattttcat
     2041 ataaaattca taaatatatg ttttttgttt tttatatatt attttctttt agtattttat
     2101 aaaacaaata gccatatact ttattttaaa tatatggcta ttctataatt taatatctaa
     2161 gctgcttctt ttattgaaac tttagttata ttacttattc tatacatttc tttgacacca
     2221 tttagagttt gcagtaagtt tagacatatt atcttgtcac ctagactttt agtatttata
     2281 atactctcta aaattacact tcttccttca tcatacttat ctacattgta atttgaaata
     2341 taattagtat ctctcaatat tttttcaaca tcttcatctt ttattttatt tttattaaca
     2401 tttatatata gattatacat attacttatt agtaatttct gattttctaa aaaatcctct
     2461 tgatgtttat ctttatatat agcatttatc aacataagtc tatcttcaat aagtgatggt
     2521 atcaataata aattttttat atagtaaaca tcagctacat tttctataga cttattgact
     2581 tcatcaatta tatttttaac tattccttta tacatttcta taacatgttg atagcctgtt
     2641 ttagcatcat atggtaatat aaacttattt actattaatg caataattgc tcctaatgct
     2701 acatatgaaa tcctgcttat ggttaaaaca tttgggtctc ccataactac tgctgaacca
     2761 agagctgaaa cagttacaca taccattaat tttctataat caactacata agagtttata
     2821 taccctgcta aaagaactat taacgacctt aatgtactat cttttattat tgaaaataat
     2881 acaataaata ttacagctcc tataaatgta ccttctattc tttttctaga cctttgtata
     2941 caattttctg cgtaaggttg tgttaatgaa aagacagtta acattatcca tcttccatct
     3001 cttaaatgga aataatccat aataaaaccc gctacagctg tagctaaagc aatctttact
     3061 gcataagaaa atcttataga atttaaatta aagtttttct tatatattga tagtcgttta
     3121 aattcatgag gtatctttga ttttctttca gctttctttt cgtctcttga ttttactttt
     3181 ttataatcta ctaaaaactc atataaattt tctaaaacac tataaatttc ttttaaattt
     3241 atatttttag tatcctgagc aataacataa tctctaaatc ctaatatctc tttatcagtt
     3301 atggtatctt tattttctaa acacatctta atatagttta tttctttcgt tgctacaact
     3361 agaacatctg atttactatt tatgctactt tcttctaaaa tattttttat atcttcatct
     3421 tcaaattctt tactatcctt tttgtatctt tctaggatta aacttattct ttctaatgaa
     3481 aataaaatat tcattatgtc tatacctcta atactgatat aaaagtcatc ttttctatta
     3541 tcaaaaataa ccattttcaa atttttagat atagaattta ttctataatt acagctatct
     3601 atttttctca tactagattt aattatagaa ctatcatcat taatagtatt ttttaaaaga
     3661 ctaatttttt ctaataattc atcacatata gatatcagtt ctttatctcc gacatttttc
     3721 atcctatttc tattcataac aaattgagca atcattatta taactgctcc aacagctaaa
     3781 gccgacaatc tcataggaaa ttcacttttt cctacaggta tacttaccat aaatatatac
     3841 attagaccaa agggtacata tactgctctt tttaagtcat aactaaatac ataccctatt
     3901 ataaataatg ctataaaatt taataaaact cctaaataca tattttgtac tgcaaaaaat
     3961 gatagtattc ctaagattat atttattgct aaatacttca gtaaattttt aattggagaa
     4021 atagttaaat ctctttccaa gagcgttagc atagctgtta ctatacctac acctataagt
     4081 gtatttttac ttccaaataa atatataaat atagaaataa atgctattat aaaaataaac
     4141 aactttgttt ttgatattac tagtttcttt gtcatcatat cccccctaaa aatttcaaca
     4201 agaccaattt tatcatattt gttatcttat tcaaaatgcg cttttttacg gtaggacttt
     4261 aacttttttt atatttagaa ctatataatt catatataaa cgtatttttt atatatataa
     4321 ccgatatatt ttcgtatgcc cccatatttt ttttgtttca aatatttttt caaaaatata
     4381 aaaataactc tctcaacaat actaaaaatg catttatcaa gagagttata atttatacaa
     4441 taaatttaat taagcttttc tttacctaat atataagttc caagacccat aaataaagct
     4501 cctccaacaa tatttccaag tgttacagct actaaattat aaatagctcc tccaatagta
     4561 actgtattta ttgttggtga aaataatgaa acactataaa ttgtcatatt tgctacacta
     4621 tgctcaaagc ctgatgttat aaaagcaaat aaacataaga atatcataat tatttttgca
     4681 gtatcactat tagttctaaa cgaacacaac actgatacac atactagaat attacataaa
     4741 atccctctaa agaaaagagc tgtaaatggc atggatgctt tactagctgc tgtatttgca
     4801 aaaaattcag ctacaggtcc tttgtctatt agaccagttc ctacaaatat aatgcctaaa
     4861 accaaagctc cgattaaatt acctacccaa ctgtatgccc atattttaga agtatctttt
     4921 atacttacac ctttatttaa cataccagca gacataacca tattgtttcc agtaaataac
     4981 tctgttcccg tcattataac caaacttagt gctattgcaa atgataaccc cattactatc
     5041 ttagtcatag gtgaacctgc atcagtcaaa agtcctccaa tagtaaatat caaaagtata
     5101 cctataccta cataaagtcc tgcaaacgca gaagatacca aatattttac tttacttgtg
     5161 tttaataaat ttatcttatt tatagctgca ttagttaatt tatccaaagt ttctttatgc
     5221 ataattccct cttccttcta ttaaatttta atttattcaa atatatgttt tactcaattt
     5281 atagatattt aattccactc cagtatatgt tttcgtacat tttagttatt tctggaagct
     5341 ctacaccttc aagtgcccat tttttaaatt ccataaatcc tgttctatca attatatatc
     5401 ctatatgttc ttttcctcct ggtgcatctt tatctatata cttttctaca tatctataag
     5461 tattaagtat tatctttatt atgctgtcct catctaccca taaaagaaaa tcttctgcta
     5521 gtctagggtt tttctttcct gttctaccca ttatagctaa tctatagtat tttttctcat
     5581 ctctagtcca tgcatttgtt gggcaattta gcacacactc tccacagcct atacacttat
     5641 ctttatctct taccacctta ttattttctg tttttaatgc tccaacagat atcttagaac
     5701 atttttttac acatgctcca caatttacac atctatcctt ttcatatatt ggtaaagcca
     5761 taccaataat tccaaagtca ttcattctag cttttataca atcatttgga caaccagtta
     5821 aagccacttt aaaatgtaaa tcatttggaa atatagcttt ttctattctt ttagcaaatt
     5881 cagttgtatt atattgagcc tttggacaga ctttatttcc tatacaagct gctatatttc
     5941 ttgtacctgc tgctggataa cctgcgtttt tctcactttg atttatattc attttttcaa
     6001 taacaggttg tataatttta tttatttctt ccatatcttc catatttatt cctaatattt
     6061 caaacccctg tcttgtagtt atgtgcacct gtccattgcc gtactcactt gcaattttag
     6121 aaactacagc taaactctca gggtcaataa gacctccagg tactctaact cttaaagaag
     6181 tctcatattt tgtttttgta attctataag catttttcat tacttttttg gtatttaaat
     6241 ctcttatcac aaacttcacc ccctaatcaa taagattttt agcatatgaa taatcaaata
     6301 ctggtccatc taaacatatg taagtatcat ccattttaca atgtccacat ttacctattc
     6361 cacaacacat ttttctttca taagaaaccc aaatgttctt ttcatctaaa tctcttttta
     6421 aaaattcttc tactgtaaat ttcatcatca ttggaggtcc aactacaact atagaagtat
     6481 tgtttatatc tttcatatcc aattctggta tgtattttgt tactaatcct acattaccac
     6541 tatatccttc ctctgctcca tctacagtaa ctaaaatatc aagtttttta ctccatctct
     6601 ttaaatcatc tgcaaataaa atatcttttg gtgatttaaa tcctactatt aatttaaact
     6661 tttcgcattt ttctttatta ttataaaaat attcaactat tcctcttaca ggggctaatg
     6721 cacttcctcc tgccactact acaatttctc ttccttcata aagggataca tcaaatccat
     6781 ttccataagg acctcttata aaaaaagaat ctccttcttt ataattaaac aactcgctag
     6841 ttacttttcc tacatttctt attgtaaagt caatataatc ctctccatat cctgatactg
     6901 atattggact ttcaccgtat ttaggtatag atatctcata aaactttcca ggcaatacac
     6961 cttttgtatt gcagtttact ctaaaagtcc attctttatc agtatgttta actatatcta
     7021 gtatttttgc acttactggt atatatgggt tcatttatac ttcctcctta ctaattacta
     7081 aatcatttat tttttctata cactttgaaa atgatatgta ttgcggacag gcatcatcac
     7141 atcttccaca gccaacacac atatgatatc cgaatctttt tttaaaatca tgtattttat
     7201 gcatagtttt aaatctcatt ctttcgcctt gtttttttct aaaagaattt ccacctgcta
     7261 tatctgtata tccatctact tgacaagaag cccaaaccct tcttctctca cctacatttt
     7321 cgttttcctt atagaaaata tcctgcattg taaaacatga acatgttgga catacaaaat
     7381 tacatctacc acatcctata catcttgtat catattcatc ccatattgaa tttcctatca
     7441 tttcatttga atcaatattc tctggcacat ctacagatat taaattatca gttacataat
     7501 caacattaaa ttcagttgct tctccattaa atacttcaaa ttcactatcc tttatatcta
     7561 agtacagtgt atctccttct atatttaatc caattgaata attatcagtt ttgttagaat
     7621 ccatgctcac acaaaaacag tttctaaaac tttccttaca ccctacaagt gcaaacttta
     7681 ctttatctct gatattttta taaaaataat ctttttcttc accattattt agatatattt
     7741 catcaagtct ttttatacca tttatgtcac aagctcttaa gaaaattaaa attttcttgt
     7801 tgtctaaatc gctttcctta aactcttttt cagtaaaata aaacaatact tgatttatgg
     7861 gaagtacaac ttcttttggt gaaaagtttg ctttcttagc aaattccatt tcttcaaaag
     7921 cgtttacctc cttgtatctt ataacatcag tatctgaaaa agtaccttta aatgggatta
     7981 aaactggtgc aaatatcttg tagtcttttt ttagataatt cagaccttca ttgaacttag
     8041 atgaatctaa ttttagtttc atttttatca cctttcttat attttagttt tacttacttg
     8101 ctatcttgtt taaatcttaa cactttattt tctatgcttc cgtgataatt atcacagttt
     8161 caaaaataat aaaaaaaata ttaatggagt ttattaagtt ctgttaatat ttttttatta
     8221 tctacattat tcgatttcaa atctaatcac ttaaatacaa tcattaagta caataaaact
     8281 actatctact caattttttg aataaaaaag aatcttattt g
//
LOCUS       DAMJYR010000149         8299 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.154, whole genome shotgun sequence.
ACCESSION   DAMJYR010000149 DAMJYR010000000
VERSION     DAMJYR010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8299
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.154"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(115..1791)
                     /gene="bclA2"
                     /locus_tag="PC471_003272"
     CDS             complement(115..1791)
                     /gene="bclA2"
                     /locus_tag="PC471_003272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-like exosporium glycoprotein BclA2"
                     /protein_id="HDF2790376.1"
                     /translation="MSDISGPSLYQDVGPTGPTGATGPTGPTGPRGATGATGANGITG
                     PTGNTGATGANGITGPTGNMGATGANGTTGSTGPTGNTGATGANGITGPTGNTGATGA
                     NGITGPTGNKGATGANGITGSTGPTGNTGATGANGITGPTGNTGATGATGPTGLTGAT
                     GATGANGITGPTGNTGATGANGVTGATGPTGNTGATGPTGSIGATGATGTTGATGPIG
                     ATGATGADGEVGPTGAVGATGPDGLVGPTGPTGPTGATGANGLVGPTGPTGATGANGL
                     VGPTGATGATGVAGAIGPTGAVGATGPTGADGAVGPTGATGATGANGATGPTGAVGAT
                     GANGVAGPIGPTGPTGANGVAGATGATGATGANGATGPTGAVGATGANGVAGAIGPTG
                     PTGANGATGATGATGATGANGATGPTGATGATGVLAANNAQFTVSSSSLVNNTLVTFN
                     SSFINGTNITFPTSSTINLAVGGIYNVSFGIRATLSLAGFMSITTNFNGVAQNNFIAK
                     AVNTLTSSDVSVSLSFLVDARAAAVTLSFTFGSGTTGTSAAGYVSVYRIQ"
     gene            complement(2169..2708)
                     /gene="hpt"
                     /locus_tag="PC471_003273"
     CDS             complement(2169..2708)
                     /gene="hpt"
                     /locus_tag="PC471_003273"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HDF2790377.1"
                     /translation="MDIETKKWEVLYSEEKIKDKLRELGAIIEKDYKDKNLMVVSLLK
                     GSFIFCADLVRNINLPLRVNFMTTSSYGNNEESTGRVKVVSDVTTDIAGYDVLVVDDI
                     TDSALTMDFVLKHLKAKNPASLKCCVLLDKPSRRKVDLVPDYCGFEIEDKFVVGYGFD
                     FGDYYRNVPYIFNVTDEDR"
     gene            complement(3036..4343)
                     /locus_tag="PC471_003274"
     CDS             complement(3036..4343)
                     /locus_tag="PC471_003274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine dehydratase subunit alpha family protein"
                     /protein_id="HDF2790378.1"
                     /translation="MRDIRKELVEMLKAEVKPAVGCTEPVALALACAKAKELLGEEIV
                     ENRMLVSPSIYKNGMCVGIPGTERLGLKIAAALGIVGGHSENGLSVLETLTKEEVKIA
                     EDYMDNTPLSITPADTREKVFIEVVLKGKNHIAKVRIRTKHDNFTFLEKDGEVLLDNE
                     PKVSASNDAAEKAESLMDTVTIQELIKNVEEIDFKDIEFLLDGVKMNEEMAEYGLKQK
                     TGIGVGYGIKKSIEEGLLGNDVINYAMMLTAGASDARMAGVKMPVMSSNGSGNHGLTA
                     ILPIVAYNKKFPQSDERLAKALAISHLVTGYIKNYTGRLSAVCGCGVAASTGATAGIS
                     WLMNGTEKQIEGAIENMIADLSGMICDGAKAGCALKLSSAASAAIQSAIIAKQDCFVP
                     PLNGIVGSSVEQSIQNLGRVSDKGMSITDEIILNVMNDMNKVD"
     gene            complement(4768..6543)
                     /locus_tag="PC471_003275"
     CDS             complement(4768..6543)
                     /locus_tag="PC471_003275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDF2790379.1"
                     /translation="MMIMIGLEYIKSDIQNIAEAIVSVLNIDVTIVNKELFRIAGTGV
                     YIDKIGEKVDKYTAFKKSLTEQITILIDDPKSSDICRECYKSGACVEFAEVCCPIICD
                     GDSHGVIGLIAFTKEQAQIIENNKSGLINFLGKMADLISNKLKAQIKTYELELEKKKL
                     ETLLNNMDKAVVSIDVDGKIDKYNSKFKELFNIKDSITGKNIFVELDFIKKPSINNFK
                     KDKSCSFFYKRYGYDSKGIYNINKIVLKNELKGYVIDFIDKRDAIKNYNKMNKDYRIK
                     LENIIGESVAIRNSKKEALIASKSTSTVLITGESGTGKELFARAIHNHSNRVDNPFIA
                     VNCAAIPDNLLESELFGYEEGAFTGAKKGGKLGMFEVAHKGSIFLDEIGDMSLHLQSK
                     LLRVLQEKELNKIGSKSNVSIDVRIIAATNKDLERMVQNGTFREDLYYRLNVIPIKLP
                     SLRERKEDIPLIIKHMVKEYSKKLDKNVIDIDSRVVDAMMEYKWPGNIRELQNIIEYS
                     VNMSSSSIITMNVIPQKIKNTSVEELEVKSDRIIPLDELEKIEIIKALNKYKDYKKDK
                     ELTAKALGISRATLYRKIEKYNIIS"
     gene            complement(6540..6677)
                     /locus_tag="PC471_003276"
     CDS             complement(6540..6677)
                     /locus_tag="PC471_003276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2790380.1"
                     /translation="MEINYELIIIVMLFVILMSIQFTLNKIYLILKEIRQILMLKKNK
                     E"
     gene            complement(6755..7324)
                     /locus_tag="PC471_003277"
     CDS             complement(6755..7324)
                     /locus_tag="PC471_003277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HDF2790381.1"
                     /translation="MKSILCQINKQPVKAKYTSDELECFLDTIEYNVGDKILLVGHIG
                     NLGKRLRGLGTSVTILENSNYEDVCYSLVYNENCNVVKGKLEFLPFDDNQFNKIIIVD
                     QFNVITDCEKASSEIQRVLKGNGEVILEDLNLKNLKVKLNYLKHKFCGSVAKIHYPHE
                     VFNIFSRLNFDGMIKESNKLKYIYVGKRR"
     gene            complement(7508..8143)
                     /locus_tag="PC471_003278"
     CDS             complement(7508..8143)
                     /locus_tag="PC471_003278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HDF2790382.1"
                     /translation="MIAVKDENNVVNLRGELDNKLEFSHEIFGEKFYNMKIKINRLSD
                     SFDILPMTVSERLLQDIDLDKQNLVNVVGQLRSYNKTLNNKNRLVLTVFVREIKSIDE
                     ENKDPNSIFLDGYVCKEPVYRKTPLGREITDLLVAINRPYNKSDYIPSIVWGRNAKFA
                     KNLKVGDRIQLWGRVQSREYEKKIDENNVVKKMAYEVSISKIKKLDENNNE"
BASE COUNT         2367 a         1656 c          954 g         3322 t
ORIGIN      
        1 atattgttta ttattttata gtcttttcat catataaacc tattaaatat tattctattt
       61 attggtatat gaaatcatat tgtttattat tttataatct tttcatcata taaactattg
      121 tattctataa actgatacat atccagctgc agaagtacct gtcgtgcctg aaccaaatgt
      181 aaaacttaaa gtaacagctg ctgctctagc atcaactaaa aagcttaaac ttacacttac
      241 atctgatgaa gtaagtgtat ttactgcttt tgcaataaag ttattttgag ctactccatt
      301 aaagttagta gtaattgaca taaatcctgc aagtgaaagt gtggcacgta taccgaaaga
      361 tacattgtat atccctccaa ctgcaagatt tatagtacta cttgttggaa aagttatatt
      421 agttccattt ataaatgatg aattaaatgt cactaatgta ttattcacta aacttgaaga
      481 ggacactgta aattgtgcat tgtttgctgc taacactcct gttgctccgg tcgctccggt
      541 tggacctgtt gctccatttg ctcctgttgc tccggtcgcc cctgttgctc ctgtcgctcc
      601 atttgctcca gttgggcctg ttggtcctat cgctcctgct actccatttg ctcccgttgc
      661 tcctactgct ccggttgggc ctgttgctcc atttgcccct gttgctcctg tcgctcctgt
      721 tgctcctgct actccatttg ctccggttgg acctgttgga cctattggac ctgctactcc
      781 attcgctccg gttgctccta ctgctcccgt tgggcctgtt gctccatttg cccctgttgc
      841 tcctgtcgct ccggttggac ctactgctcc atctgctccc gttgggcctg ttgctcctac
      901 tgctcctgtt ggacctattg ccccagctac tcctgttgct cctgtcgctc ctgttggacc
      961 taccaaacca tttgctccag ttgctccggt tgggcctgtt gggcctacca aaccatttgc
     1021 tccagttgct ccggttgggc ctgttgggcc tgttggacct accaaaccat ctggacctgt
     1081 tgctcctact gctcctgttg gacctacctc tccatctgct cctgttgctc cggttgctcc
     1141 tattgggcct gttgccccag ttgttcctgt tgctcctgtc gctcctatac ttccggttgg
     1201 acctgttgct cctgtatttc ctgttgggcc tgtagcacct gttactccat ttgctccggt
     1261 tgctcctgta tttcctgttg gacctgttat tccatttgct ccggttgctc cggttgctcc
     1321 agttagtccg gttggacctg ttgctccagt tgctcctgta ttccctgttg gacctgttat
     1381 tccatttgct ccagtcgctc ctgtatttcc tgttggtcct gtagagcctg ttattccatt
     1441 tgctccagtt gctcctttgt ttcctgttgg acctgttatt ccatttgctc cggttgctcc
     1501 tgtattccct gtcggacctg ttattccatt cgctccagtc gctcctgtat ttcctgttgg
     1561 tcctgtagag cctgttgttc catttgctcc agtcgctccc atatttcctg ttggtcccgt
     1621 tattccattc gccccggttg ctcccgtatt tcctgttgga cctgttattc catttgctcc
     1681 ggtcgctcca gttgcacctc taggccccgt cggtcctgtt ggaccagtag cacctgttgg
     1741 ccctgttgga cctacatctt gatataaact tggacctgaa atatcactca tataattaat
     1801 cctccttttt taaagttaga gtattactct ataaatattg agtattactc tatataatta
     1861 tatgaatcga caatttctta tgtgataatg tatctatttg cttatatcaa catatatgct
     1921 ttgattcttg aatttcatta cttttttgta aaatctttta caattattag tactctttta
     1981 caattattaa tacaattaaa aaactcattt tccatcaatt atttaaaatt tttttattat
     2041 gttataaaaa atagataatt aaattgcata aaattaattt aatactactt taattacttt
     2101 gtaaataaaa aaacctcaac acgattaata aaaatcatat taaggcatcc acaaaattat
     2161 ataattgctt atctatcttc atcagtaaca ttgaatatat aaggaacatt tctataataa
     2221 tcaccaaaat caaacccata tccaaccaca aacttatctt ctatttcaaa tccacaataa
     2281 tctggcacta aatcgacttt tcttctgctt ggtttatcca aaagaacaca acattttaaa
     2341 ctagcaggat ttttagcttt taaatgtttt aacacaaaat ccatagtaag tgcagaatca
     2401 gtaatatcgt ctacaaccaa aacatcatat cctgctatat cagtagtcac atctgaaact
     2461 actttaaccc ttccagtact ttcttcattg tttccataac tagatgtagt cataaaatta
     2521 actctaagtg gtaaatttat atttctaact aagtctgcac aaaatataaa gctacctttt
     2581 aatagagaaa ctaccataag atttttatct ttataatctt tttctataat tgctcctaat
     2641 tcccttaatt tatctttaat tttttcttct gaatacaata cttcccattt ttttgtttct
     2701 atatccataa aaagacctcc aattgtaata atccttctta attttataag agattgctca
     2761 ctttttcaat actcttattc aatattagtc aatattattg agtttttatg ttcattcaac
     2821 aattattaac aatgtatctt atattttaag tagttcatat atccacacta aattacatac
     2881 tatatatttt agacaaaaaa agaaaaaact tgcactaaca agttttttct attatacata
     2941 aatttataca taatcatatt ttattagagg gtactaatta aatttattta tgctattttt
     3001 gttatacctc ataatacaaa acatattata aagtactagt caactttatt catatcattc
     3061 attacattta agattatctc atctgttata gacatacctt tgtcacttac tctacctaag
     3121 ttttgtatac tttgctcaac acttgaacca actataccat ttagtggtgg tacaaaacaa
     3181 tcttgcttag ctattatagc actttgtata gctgcagatg ctgcagaaga taattttaat
     3241 gcacaaccag ctttcgcacc gtcacatatc attccactta agtcagctat catattttct
     3301 atagctcctt ctatttgctt ttcagtacca ttcataagcc aagagatacc agcagttgct
     3361 ccagtagaag cagcaacacc acatccacat acagcagata atcttcctgt atagttctta
     3421 atataaccag ttactagatg agatatagct aaagcttttg ctagtctttc atcagattgt
     3481 gggaattttt tattataagc aacgattgga agaattgctg ttaatccatg atttccactt
     3541 ccatttgaac tcataacagg catttttacc cctgccattc ttgcatctga agctccagca
     3601 gttaacatca ttgcataatt tataacatca ttacctaaaa gaccttcttc tatagacttt
     3661 ttaattccat acccgacacc aataccagtt ttttgtttta atccatattc agccatctct
     3721 tcattcattt ttactccatc tagtaaaaat tctatatctt taaaatcaat ttcttctaca
     3781 ttttttatta attcttgtat agttactgta tccattaaac tttcagcttt ttctgccgca
     3841 tcatttgaag ctgatacttt tggttcattg tctaataata cttctccatc tttttctaag
     3901 aacgtaaaat tatcatgttt agttcttatt ctcactttag caatatgatt ttttcctttt
     3961 aacacaactt ctataaatac tttctctctt gtatcagcag gtgttattga tagtggagta
     4021 ttatccatat aatcctcagc tattttaact tcctcttttg ttagagtttc taacacactt
     4081 aatccatttt cagaatgtcc acctactata cctagagctg ctgcaatttt taatcctaat
     4141 ctttcagtac ctggtatacc tacacacata ccatttttat agatactagg actaactaac
     4201 atacgatttt caactatttc ttctcctaat aattcctttg cctttgcaca tgctagagct
     4261 aaggctactg gttcagtaca ccctactgct ggtttaactt ctgcctttaa catttccact
     4321 aattcttttc ttatatctct cattatatat aaacctccca attaagttta gatttagtac
     4381 tcaaataatt agttaaataa tttcttatct atatttatag caaaatacat gccatatttt
     4441 ttatatcttt ttatattttt agtcttatat tctataacca ttggtatgac tgcctctatt
     4501 tatgtatttt ttttatttgt atagagtatt tatattcttt aaaatggaga aacatatgac
     4561 aatttcgcaa aaagacaaaa aaaagtctca aaatgagatt ctatatcatt ttgagacggc
     4621 tcaaatatat tattttttaa cttataaaaa ccatttttta attcataaaa aaagattctg
     4681 caaaacactt atagtataag tttaaagctt tctttaaagt tatgaattta gaaaaattta
     4741 aattttatat agctttttat tatattacta tgaaattata ttgtattttt caatttttct
     4801 atataatgta gctcttgata ttccaagtgc tttagctgtt aattctttat cttttttata
     4861 atctttatac ttatttaaag cttttattat ctcaatcttc tctagttcat caagtggaat
     4921 tatcctatcg cttttaactt ctaactcttc tacagaggta ttcttaattt tttgaggaat
     4981 tacattcatt gtgattattg aagaagaaga catattcaca ctatattcaa taatattctg
     5041 caactctctt atattccctg gccacttata ttccatcata gcatcaacaa ccctgctatc
     5101 tatgtcaata acatttttat ctaatttttt tgaatactct ttaaccatat gcttgattat
     5161 aagtgggata tcttcttttc tctctcttaa acttggtaat tttataggta taacatttaa
     5221 tctataatat aaatcttctc taaaagtgcc attttgaacc attctttcta aatctttatt
     5281 tgtagcagca ataattctta catctattga aacatttgat ttacttccaa ttttattaag
     5341 ttccttttct tgtaatactc tcaataactt tgactgtaaa tgcaaactca tatctcctat
     5401 ttcatctaaa aatatacttc ccttatgagc tacttcaaac attccaagct ttccaccttt
     5461 tttagctcca gtaaaagctc cctcttcata tccaaataat tcactctcta ataaattatc
     5521 cggtatagca gcacagttta cagctataaa aggattatct actctattgc tatggttgtg
     5581 tatggctctt gcaaatagct ccttacccgt tccactttcc ccagtaataa gtactgttga
     5641 tgttgattta gaagctatta gggcttcctt tttagaattt cttatagcta cactttctcc
     5701 aattatgttt tccaatttta ttctataatc tttattcatc ttattgtagt tttttatagc
     5761 atctctttta tctataaaat caataacata tccctttaac tcatttttta atactatttt
     5821 attgatgttg tatattccct tcgaatcata tccgtatctt ttataaaaaa aactacagga
     5881 tttgtccttc ttaaagttat ttatagaagg tttctttata aaatctaact cgacaaatat
     5941 gtttttgcca gttatactat cttttatatt gaaaagctct ttaaattttg aattatattt
     6001 atcaattttt ccatccacat caatagacac aacagctttg tccatattgt ttaatagtgt
     6061 ctctaatttt ttcttttcta attcaagctc ataagtttta atttgtgctt ttaatttatt
     6121 agatattagg tctgccattt tgcctaaaaa gtttataaga ccacttttat tattttctat
     6181 aatttgtgct tgttcttttg taaaggctat aaggcctata acaccatgag aatcgccatc
     6241 acatataata ggacaacaca cctctgcaaa ctctacacaa gccccacttt tatagcattc
     6301 cctacatata tcactactct ttgggtcatc aattagtatt gtaatctgtt ctgtaagtga
     6361 ttttttaaaa gccgtatatt tatctacttt ctcacctatt ttgtctatat atactcctgt
     6421 acctgcaatc ctaaataact ccttatttac tatagttaca tctatattga gtactgatac
     6481 tatagcttca gctatatttt gaatatcaga ttttatatat tctaaaccta tcataatcat
     6541 cattccttat ttttctttag cataagtatt tgtcttattt ctttaagaat caagtatatc
     6601 ttatttaaag taaattggat tgacatcagg attacaaaga gcataacaat tattattaac
     6661 tcataattta tttccatcta aacatctcct tataacaaaa gactaggctt aacctagtct
     6721 tttatattat atccatattt atattttaac tcatttatct tctttttccc acataaatat
     6781 attttaattt atttgactct ttaatcatac catcaaagtt taatcttgaa aatatattaa
     6841 atacttcatg tggatagtga atctttgcaa cagaaccaca aaatttatgt ttaagatagt
     6901 ttaacttaac ttttaaattt tttaaattta aatcctctaa tataacctct ccattgccct
     6961 ttaacactct ttgtatttca gatgaagctt tttcacaatc tgtaataaca ttaaattggt
     7021 caactattat tatcttgttg aactgattat catcaaatgg tagaaactct aacttacctt
     7081 taactacatt gcaattttca ttatacacta gagaataaca gacatcttca taattactat
     7141 tctccagtat agttacagaa gttccaagac ctctcaatct cttgcctagg tttcctatat
     7201 gaccaacaag caatatttta tcacctacat tatactcaat tgtatccaaa aaacattcta
     7261 attcatcaga tgtatattta gctttaactg gctgtttatt tatctgacat aatatgcttt
     7321 tcacaatgct tcacctcttc atttcctcaa ttataaatta tgtacagatt acattataat
     7381 aaatggcgag caactattca agagatattt cattagtatt tttttaaaca atctttatac
     7441 attttaatat gatattatac caaatttatt aggattttat tcataaaaat cagtctattt
     7501 tttatgttta ttcattgtta ttttcatcca atttttttat tttcgatatg gatacttcat
     7561 atgccatttt tttgaccaca ttattttcat ctattttttt ctcatattct ctactttgaa
     7621 ccctacccca aagctgtatt ctatcaccaa cctttagatt ctttgcaaat ttagcatttc
     7681 taccccaaac tattgatggt atataatcag atttattata aggtctgttt atagcaacta
     7741 ataaatcggt tatttccctt cctaaaggtg tcttcctata cacaggttcc ttacatacat
     7801 atccatctaa aaatatacta ttagggtctt tattttcctc gtctatactt tttatttctc
     7861 tgacaaacac tgtaagcact aatctatttt tattatttaa tgttttgtta tatgatctta
     7921 attggcctac gacattgact aaattttgtt tatcaaggtc aatatcttgg agaagtcttt
     7981 ctgatacagt cattggaagt atatcaaaag aatcgcttag tctgtttatt tttattttca
     8041 tattataaaa tttttctcca aatatctcat ggctaaattc tagcttattg tctaactccc
     8101 ctcttaagtt tactacgtta ttctcatctt taacagctat catattttat ccctccacaa
     8161 ttgttaattc ttatatttct ggtatcagct tatataataa atatatttat aagggatgta
     8221 ttttattaca aatctttgat tgctgtttaa aagtttttat ttagttataa atttcattta
     8281 atatttcact tttataatt
//
LOCUS       DAMJYR010000150         8256 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.155, whole genome shotgun sequence.
ACCESSION   DAMJYR010000150 DAMJYR010000000
VERSION     DAMJYR010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8256)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8256)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8256
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.155"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            133..1026
                     /locus_tag="PC471_003279"
     CDS             133..1026
                     /locus_tag="PC471_003279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HDF2790383.1"
                     /translation="MQLYNTPFNTNKHLIYFFTFTLVLNWIFSLTPALLGITNTILGE
                     IIFYLAYTSPAIVGLFFISTIYPQSAKQDFIYRYLSFKEIGIKWILLTILFFALTFIT
                     SLIIGNYFNVKTSDIDWIHIAVFRPSKIAYMLLASFILSILSQEAGWRGYAIDKLLVR
                     FGFIGSSIILGLVCGIWYLGSYFTPNQIPYNLAQYSLFDAFLFIPNIVLLNFIINFVY
                     INTSRSILSAGLVHMMYYFFNIQLLLHYPIKLCVIAQYVQIIFGLIFLVYMISSNKFK
                     QKVNSEIEQIKSDDFEFELDW"
     gene            1204..2028
                     /locus_tag="PC471_003280"
     CDS             1204..2028
                     /locus_tag="PC471_003280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDF2790384.1"
                     /translation="MFKIGEVSKLTQISIRMLRYYDELGILKPAKTDKYTGHRLYSVE
                     QISILQRIVLLRDSKFSVAEIANIVHNWNDEFVIKELNRKKNEIQKEIKQEQQRINKI
                     DKFIEAINCDKDEIHYNVVFKKIPSYKIISLREIIPDYQSEGILWEKLSKFIKEEHIE
                     VSRQSNNNIAFYHDEEVKDNGVDIEVGMVVKKIGKNKSGFIYRETEEIDMMACTMVYG
                     PYENIAGAYESFCYWLDKNSDYQISGINRQIGHKGEHNEINPENYLTEIQIPLIKV"
     gene            2127..2735
                     /locus_tag="PC471_003281"
     CDS             2127..2735
                     /locus_tag="PC471_003281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDF2790385.1"
                     /translation="MEKLLRGKIMKADSTKEQLINATIQLLSSHRDVSSITAREIVAK
                     ANVNLAMINYYFKSKEELINISIKKFLKRHMDEYKATHDTKNLAPKQQLREILIAFFD
                     FIGEHSQFIKISVPYILIQEEIVYPFEILPLIKAHYENQNNKKTEAEYRTIAYQITSF
                     IQLLFLRSNAFFKYSGIDILNIEQRNGLIDFQLDLLLVDITK"
     gene            2839..3015
                     /locus_tag="PC471_003282"
     CDS             2839..3015
                     /locus_tag="PC471_003282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2790386.1"
                     /translation="MFFTFIGVISAILLLCTIICGLWIKLNNRNNDSNSHFTFSILSI
                     AFSLLIVVAFLVRN"
     gene            4281..5033
                     /locus_tag="PC471_003283"
     CDS             4281..5033
                     /locus_tag="PC471_003283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2790387.1"
                     /translation="MLKVNQLCKSYKTGSKTYPVLKNISFEINKGEFVAIMGSSGSGK
                     TTLLNCISCFIPIDNGEIILGNNNIRNLKEKELSEVRNQQLGFVFQDFMLFDGLSVFE
                     NICVPQIIKGSQVLQMENRAINLCELFGIEHIRKKYPAEISGGEKQRTAVARALMNNP
                     DLILADEPTGNLDSKSCRTVIESFLKAKQELDATILMVTHDSFAASFCDRVIVLKDGI
                     IYKEVVKSNSRHKFLDELLEVLKEMGGAQDDN"
     gene            5023..7134
                     /locus_tag="PC471_003284"
     CDS             5023..7134
                     /locus_tag="PC471_003284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2790388.1"
                     /translation="MTINKLYSKLRAYNWKNYTTLFFCIILSVVLVTSYALIYFSPTI
                     QNILPTGGDSRKQATMIFSVAILGCAVFTTYASSLFLKFKSKEIGIFMALGTDKKKLK
                     KLLFSEILLISIVCCFLGLILSIPVSFGIWKIFQLVIIDTREMTYHFGTQGFIYGFIF
                     SVFVTLCIFILSVKFINRTNIIDILNDQRISEPIQDVKDWYGILGIIFIILGIFLGYL
                     VPSLTINKLNYKLPSIWSFTYLLSLIGIYMIMTYIVVHAKRGKHPEKYYKNIISTSMM
                     RFMGRQTVKNMCVISFLIAGALFAVFYVPNMVTGIYDTLKNNPIDYTFTYNQRDKQVS
                     KKDIEQLADKHNIKIQNYMEIPSIELIVDGVESIYHKDGTIEKVYMKKSSYDMFFNSK
                     DFSKLIGKPITIKNGEYLAVVNKGNENQDYKYTKFISSPVTEKEIPFRYAGKIIFNGS
                     FFQNRSMNAYVLNEEDYKSFTKDLSIKNYNNSILFNVDKDTYAFANDLKKEIIKRTSK
                     EMGMFSGYDEYVKKRYESTGRKYFMDEEYPSGEGHLKLDPNNNQLYLNWKYYPNFKIL
                     QSQDMIKNMAVFLMLFIYVAIICFTAVGIIAYTRGVTIAINHKQVFMDLNRLGANNDY
                     IKFCIRNQLKKVFSYPLIAGSFLIYLFNFMIMLNNDGRIVNSEVNALLINLGFIGICG
                     VYMYLIYLLTYKKFKKIIGIL"
     gene            7416..8126
                     /locus_tag="PC471_003285"
     CDS             7416..8126
                     /locus_tag="PC471_003285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2790389.1"
                     /translation="MTSICICDDEKQQRNMLNKIITREMDLWGYPYQIKEYSNGESLV
                     YNLEHENYYIDIIFLDIELGKSNGVDIAKTIRKHSKDTIIIFVTGFSDYVFHGYDVGA
                     LNYILKPYKTEKICKVLKEALKKLEQWKENFITVQIGTNLCKINTKDILYFKSELRKL
                     TVVTFDKTLEYYGKLNDMETVLPSNFIRTHQRYIVNLNHVDSITQTYAKVQNEKIPIS
                     RKKYQEVVGKFTRYMLES"
BASE COUNT         3118 a         1070 c         1093 g         2975 t
ORIGIN      
        1 aatcaaaata taaaaaatag gatattatta taggcaataa ctaacatcaa gcgataactt
       61 aatacatata aaaataaata aacaattaat aaaaattaaa acttcattat aaaacggaag
      121 gatagtgaaa aaatgcaact atataacacc ccctttaata caaacaaaca cttaatctat
      181 ttctttacat ttactctagt attgaactgg atattcagtc ttacacctgc gttgctcgga
      241 attacaaata caatacttgg agaaatcatt ttttatcttg catatacatc accagctatt
      301 gtaggtctat tttttatttc tacaatctat ccacaaagtg caaaacaaga ttttatttat
      361 agatacctaa gctttaaaga aatcggaata aaatggatac tattaacaat attatttttt
      421 gctttaactt ttattacttc gttaataatc ggaaattact ttaatgtaaa aacttctgat
      481 atagattgga tacacatagc agtgtttaga ccttctaaaa tagcttatat gctattagca
      541 agttttatat tgagtatact tagtcaagaa gctggatgga gaggatatgc cattgacaaa
      601 ttattagtta gatttggatt tataggctct tcaattattt tagggcttgt ttgtggaatc
      661 tggtatctag ggtcgtattt tacacctaat caaataccat ataatttggc acaatactcc
      721 ctatttgatg cttttttatt tataccaaac attgtacttt taaactttat aattaatttt
      781 gtatatatca atacaagtag aagcatttta tcagctggat tagttcatat gatgtactat
      841 tttttcaaca ttcaattact tctacactac cctattaaat tatgtgtaat agctcaatat
      901 gtacaaataa tatttggttt aatatttctt gtatatatga tttcttctaa taaatttaaa
      961 cagaaggtaa attctgaaat tgaacagata aagtcggatg attttgaatt tgagttagac
     1021 tggtaatttt aatatgaatt ataaattaag ataatctatg atttgatacg attactactt
     1081 aaatataatt tactctctat aatttattgc aacatctgga aaataattgt atttccacaa
     1141 acgcttgaag tatataaaaa caaaatatat actgattata agtgaaaagg aggtcatatt
     1201 ttcatgttta aaataggaga ggtttcaaaa ctaacacaaa tttctattcg tatgttgcgt
     1261 tattatgatg aacttggtat tttaaaacct gcaaaaacag acaaatatac aggtcataga
     1321 ttatactcag ttgaacagat ttcaatatta caaagaattg tattattgag agattcaaaa
     1381 tttagcgttg cagaaatagc aaatatcgta cataattgga atgatgaatt tgtaattaag
     1441 gaattgaata gaaaaaagaa tgaaatacaa aaggaaataa agcaggaaca acaaagaata
     1501 aataaaattg ataaatttat agaagctata aactgtgata aagatgaaat tcattacaat
     1561 gttgtcttta aaaagattcc aagttataaa atcatatcac ttagagagat aattccagac
     1621 taccaatctg aaggtatact ctgggaaaag ctatctaaat ttatcaaaga agaacatata
     1681 gaagtatcac gtcaatccaa caataacata gctttttacc atgatgaaga agtcaaagat
     1741 aatggtgtag atattgaagt tggaatggtt gtaaagaaga ttggaaagaa caaatctgga
     1801 tttatatatc gtgagacaga agaaattgat atgatggcat gtactatggt ttatggtcca
     1861 tatgaaaata tagctggtgc atatgaatcg ttctgttatt ggttagataa aaatagtgat
     1921 tatcaaatat caggtataaa tagacaaatc ggacataaag gtgaacataa tgaaataaat
     1981 cctgaaaatt atcttacaga aattcaaatc ccccttataa aagtctaata gattagtact
     2041 taaatactta agtactaatc acccattgac atcccaaaat tttcatgata gtatctaaac
     2101 gtgtattaaa catgtttaat ttttttatgg agaaactttt aaggggaaaa attatgaaag
     2161 cagattcaac aaaggaacaa ttaattaatg ccacaatcca gttactatct tcacatagag
     2221 atgtttcaag cattacggct agagaaattg tagctaaagc aaatgtcaat ttagctatga
     2281 ttaattatta ttttaaatct aaagaggagt taattaatat ttctattaaa aaatttttaa
     2341 agcgtcatat ggatgaatat aaggctacac atgatactaa aaatttagca cctaaacagc
     2401 aattaaggga aatactaatt gcattttttg attttatagg tgaacatagt caatttataa
     2461 agatttctgt accctatata ttgatacagg aagaaattgt ttatccattt gagatactac
     2521 ctttaattaa ggctcactat gaaaatcaga ataacaaaaa aactgaagct gaatacagaa
     2581 ctattgcgta tcaaataact tcatttattc agttgctttt tcttcgttct aatgcttttt
     2641 tcaagtatag tggtattgat attcttaata ttgaacagag aaatggttta atagattttc
     2701 aattagattt attattggta gatattacaa aataatacta tattaatttt ttatgtacat
     2761 ttttttattt tatggtaacg tttgaaaata ttctcattga ctaaaaatac ttgcattaaa
     2821 taagggggag gaattttaat gttttttaca tttataggag taatatcagc aattttattg
     2881 ttgtgtacaa ttatatgtgg tttatggata aaacttaata atcgtaataa tgactctaat
     2941 tctcacttta ctttttcaat actttcaatt gctttttcac tgttaattgt ggtggccttt
     3001 ttagtaagaa attaatgtct ttgttatgct gaggtttatt attatagatt tttattattg
     3061 catgattttt actatattat agtattttta ttatattaca gggtcttatt tattatagtg
     3121 ttttgttgtt acagcatttt attatattac agagtcctat ttattatagg atttttatta
     3181 tattgtaaaa tcttatttat tataggattt tgttatatta cagactttta ttatattgaa
     3241 atctcttatt tattgcagtg tttctattat attgcaaact cttatttatt atggtgtttt
     3301 gttattgcaa catttctatt atattgtaaa gtcttattta ttacagttct ttattatact
     3361 acagaccttt attattacag catttctatt acattgtaaa gtcttattta ttacagttct
     3421 ttattacact acagactttt attattacaa tctcttattt attacagttc tttgctatac
     3481 tacagacctt tattattaca gcatttctat tacattgcaa agtcttattt attacagttc
     3541 tttattatac tacagacttt tattattaca gcatttctat tacattgcaa agtcttattt
     3601 attacagttc tttattatac tacaaacctt tattattaca acattttatt attttaactt
     3661 ctttactata atatagtaat attttaatat ctcaaaatca tatcaatcac ttttcagcat
     3721 agtctaaaat acttccactt tctaaaatta tctatcatac ctatactaat aatacttttt
     3781 aaccttttta ttatacattt caattgtttc taacaaccta tttatttcca aaactccaat
     3841 atattttctc aattacattg tgttaatagc ccctaaatcc tttcaaatta tttacaatac
     3901 attccattaa ataatcttac tgctctataa ttcttttttt ctttttaacc ctactataca
     3961 ttatttacat catttttttc aatacaacat tcgttattct ataacctttt tacttttact
     4021 atatacttct tttctaatta caccttcaat gttccacatc atattacttt agcacaacaa
     4081 ttaataccta aaaataaccc tctaaatcct ccaataatat aatcaattta actataataa
     4141 taacctctca cactatccaa acaatatatt ttcaaacatt acaaaaaaac atccaactat
     4201 tacaatatag ctcatatccc tcctcccctt tcaaatgcta tcattgactt acaagataaa
     4261 tgaaagtgag gatgatatat atgcttaaag taaaccaatt atgtaaatca tataaaacag
     4321 gcagtaaaac atatcctgta ttaaaaaata tttcttttga aatcaacaaa ggagaatttg
     4381 tcgcaatcat gggttcatca ggaagtggaa aaactactct tttaaattgt atttcatgtt
     4441 ttatcccaat tgataatggg gaaataattc ttggaaataa taatataaga aatttaaaag
     4501 aaaaggaact ttcagaagtt agaaatcaac aactaggatt tgtattccaa gacttcatgc
     4561 tatttgatgg tcttagtgtc tttgaaaata tatgtgttcc acaaattatc aaaggtagtc
     4621 aagtcttaca aatggaaaac cgtgcaatta atctatgtga gctatttggt attgaacata
     4681 tcagaaaaaa ataccctgcc gaaatatctg gtggagaaaa acagcgtaca gcagtagcaa
     4741 gagcactcat gaacaaccca gatttaatat tagcagatga accaactggt aaccttgata
     4801 gtaagtcttg tcgtacagtc attgaatcat ttttaaaagc aaagcaagaa ttagatgcta
     4861 ctatactcat ggttacacac gatagctttg cagcaagctt ctgtgaccga gttattgtgc
     4921 ttaaagatgg aataatttat aaagaagttg taaaatcaaa ttcaagacat aaattcctag
     4981 atgaattact agaagtttta aaagaaatgg gaggcgctca agatgacaat taataaatta
     5041 tacagtaaac ttcgagccta taattggaag aactatacaa ctttattctt ttgtataata
     5101 ctttctgtgg tattagtaac ttcatatgct ctaatttatt ttagcccaac aatacaaaac
     5161 atacttccaa ctggtggaga tagcagaaaa caggctacaa tgatattctc agttgctata
     5221 ttaggatgcg ctgtctttac tacatacgct tcttccctct ttttaaaatt taaaagcaag
     5281 gaaattggta ttttcatggc tctaggaaca gataagaaga aattaaaaaa acttctattt
     5341 tcagagatat tgttaatttc aatagtatgc tgttttttag gtcttatatt aagtattcct
     5401 gtatcttttg gtatatggaa aatatttcaa ttagtgatta ttgataccag agaaatgaca
     5461 tatcattttg gaactcaagg attcatatat ggatttatat ttagtgtatt tgtgacatta
     5521 tgcatattta tactttctgt aaaatttatt aatcgtacaa atataattga tatattaaat
     5581 gaccagagaa tatctgaacc aatacaggat gtaaaggact ggtatggtat attaggtata
     5641 atttttatta tactcggtat ttttctagga tatctcgtac cttcactaac tataaacaaa
     5701 ttaaattata aactaccatc tatatggagc tttacttatc tactctctct tattggaata
     5761 tatatgatta tgacatacat agttgtacat gctaaaagag gaaaacatcc agagaaatac
     5821 tataaaaaca tcatatcaac tagcatgatg agatttatgg gaagacaaac agtaaaaaac
     5881 atgtgtgtta tttcattttt gattgcagga gcattatttg ctgtattcta tgttcctaat
     5941 atggttacag gtatctatga tactcttaaa aataacccta tagattatac ttttacctac
     6001 aatcaaagag ataaacaggt atcaaaaaaa gatattgaac aattagcaga taaacacaat
     6061 ataaaaattc aaaactatat ggaaattcct tctatagaat taattgttga tggtgtagaa
     6121 tcaatatatc ataaagatgg aactatcgaa aaggtatata tgaaaaaaag ctcctatgat
     6181 atgttcttta attccaaaga tttttccaag ctaattggca aaccaataac tataaaaaat
     6241 ggtgaatatc ttgctgttgt aaataaagga aatgaaaatc aagattataa atataccaaa
     6301 tttatatcaa gcccagtcac agaaaaagaa atccccttta gatatgctgg aaaaataata
     6361 tttaatggtt ctttttttca aaatagaagt atgaacgcct atgtccttaa tgaggaggat
     6421 tataaatcat ttactaaaga tttatccata aaaaattaca ataattctat actatttaat
     6481 gtagataaag atacctatgc ttttgccaat gacttaaaga aagaaattat aaaaagaaca
     6541 tctaaggaaa tgggcatgtt cagtggttat gatgaatacg taaaaaaacg ttatgaatca
     6601 actggaagaa aatattttat ggatgaagaa tatccaagtg gtgaaggtca tttaaaacta
     6661 gacccaaaca acaatcagtt gtatcttaat tggaaatact atcctaactt taaaatttta
     6721 caaagccaag atatgataaa aaacatggca gtcttcttaa tgttattcat atatgtggca
     6781 atcatatgtt ttacagcagt aggaatcatt gcatatacaa ggggtgttac tatagctata
     6841 aatcacaagc aggtctttat ggatttaaat cgattaggag caaacaatga ttatataaag
     6901 ttctgtatta gaaatcaatt aaagaaagta ttttcatacc ctcttattgc aggaagtttt
     6961 ttaatttatc tgtttaattt tatgattatg ttaaataatg atggtagaat cgtcaatagt
     7021 gaggtcaatg ctcttttgat aaacttaggt tttataggga tttgtggggt ttatatgtac
     7081 cttatatatc tattaactta taagaaattc aaaaaaatta ttggtatttt ataataaaaa
     7141 aggttattag atttttaaag taataaaatc attacttttt ataataataa aagcatttgt
     7201 attttataat aagaaagatt gttagatttt ttaataagaa aatcattagc acttcagaat
     7261 aagtttaata gaatagtttt aagtgtaagt atactaatat aaaaaatttt attttgctat
     7321 ctggatataa ttgttatttc attttatata tctaggtagc aaaatatatt atatacttaa
     7381 ctatataaat ataaaacttg aaaggagata aaaaattgac ttcgatttgt atttgtgatg
     7441 atgaaaaaca acaaagaaac atgctgaata aaatcattac aagagaaatg gatttgtggg
     7501 gctaccccta ccaaataaaa gaatattcaa atggagaaag tctagtatac aatctagaac
     7561 acgaaaatta ttacatagat attatttttt tagatattga acttggaaaa agtaatggcg
     7621 ttgacatagc aaaaactata cgaaaacatt caaaagatac tataatcatc tttgtcacag
     7681 gtttttctga ctatgtattt catggatatg atgtaggagc tttgaactac atcttaaaac
     7741 cttataaaac tgaaaaaata tgtaaagtat taaaagaagc actaaaaaaa ttagaacaat
     7801 ggaaagaaaa ctttattaca gttcagattg gaactaatct ttgtaaaatt aatacaaaag
     7861 atatcctcta ctttaaaagt gagctaagaa aattaactgt agttaccttt gataaaacat
     7921 tagaatacta cggaaaatta aatgatatgg aaactgttct tccctctaat tttatacgta
     7981 cgcatcaaag gtatattgtc aatctaaacc atgtagatag tataactcaa acttatgcca
     8041 aagttcagaa tgaaaaaatt cctataagcc gtaaaaaata tcaagaggtt gtaggaaaat
     8101 ttacaaggta tatgttagaa tcttaatttt tgaagttcaa tctctagaat tttatttttg
     8161 aaacttaact ttttaaagct tagtttttag agttttgttt tttcaaattt aattttttga
     8221 agcttaattt ctagaatttc aatctttcaa aattta
//
LOCUS       DAMJYR010000151         8173 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.156, whole genome shotgun sequence.
ACCESSION   DAMJYR010000151 DAMJYR010000000
VERSION     DAMJYR010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8173)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8173
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.156"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(406..1047)
                     /locus_tag="PC471_003286"
     CDS             complement(406..1047)
                     /locus_tag="PC471_003286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase"
                     /protein_id="HDF2790390.1"
                     /translation="MNNNFQDNQTINLIQSRRSIRKFTTEQISDEQVNTLLHCAFAAP
                     SGCNKQPWHITVVQDQKLLKEISDDTLSRIHEVSNVEINKNFKLFYGAPTVLFISYDE
                     SSSWAPYDIGILTGNITTAAQALGLGSCIIGMVRGLFTPVEQGDIEGLVSVLDKEDVK
                     ESESIKMKFDTNKKYRELLDIPEGYSVPFGIAVGIPDGNLPNAREVVYKVSRV"
     gene            complement(1290..2093)
                     /locus_tag="PC471_003287"
     CDS             complement(1290..2093)
                     /locus_tag="PC471_003287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HDF2790391.1"
                     /translation="MNKKGFTLIELLVVISIIGILVIVAVPALFRNIEKSKAVTCLSN
                     RENIKTQIVIAMAEESSKGKNEVMKEVLENKDGKYFETEPKCKSGGIYSATFDDGYDG
                     ITGIESIAKVYVTCTKHPDGVEMARDIHQSMKDLIASFAQDPSIIPGASKGNDDFRKY
                     LLDNKYKNGWPTIPDEFKAKYGLSKDTLYIQPYAYNPTKSDATVVVFANNKTGGNWYT
                     SLVYDYDEGRWYKGKNGISVAGRSWDVDTDSVKSVKTEIHSKEGWGPLN"
     gene            complement(2249..3424)
                     /gene="thiI"
                     /locus_tag="PC471_003288"
     CDS             complement(2249..3424)
                     /gene="thiI"
                     /locus_tag="PC471_003288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HDF2790392.1"
                     /translation="MVYNILIVKYGEIGVKGKNRYIFENRLIRNIRNMLKPIGRFNVY
                     KEYGRIYVDLEDYDYEEVIEEVRKVFGIVGVCPGVRAKKDYDTLKEIALKMLEEKIEA
                     GYKTFKVESRRGDKSFGLTSQEMSMDIGGYLLSKVGDRINVDVRNPEVKIKCEYREFH
                     TMVYSDTIPGYGGLPLGTNGRAMSLLSGGIDSPVATWMVAKRGMEVEAVHFHSYPFTS
                     ERSQEKVKDLAKILAKYCGRVRLHKVNILEIQKAIGENCNEEESTILSRRFMMRIAQR
                     LSEKRHCDALITGESIGQVASQTIQGLTCTNAVVDLPVFRPLIAMDKSDIVDIAKKIG
                     TFETSIVPEEDCCSVFSPRKPVTKPRLEKIEKSETALDIEKLVQDAIDKIEVEDIEF"
     gene            complement(3470..4621)
                     /locus_tag="PC471_003289"
     CDS             complement(3470..4621)
                     /locus_tag="PC471_003289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HDF2790393.1"
                     /translation="MEIYLDNSATTKPYQEVIDKMVYALNTEYGNPSSVHRKGVEVEK
                     AIKEVRQDIAKSLGAKEKEIYFTSGGTECNNTIIRGITSLNKKRKNHIISTNIEHPSV
                     LNTLKDLEEDGFEVTYLEVGKDGKINIEDLKNAIKSTTCLVSMMHVNNEIGTIQPIGE
                     VGKYLKGLKEKIYFHVDAIQSYGKINFRPSKYNIDFMSVSAHKFHGPKGIGFMYIKEN
                     NRLKPMLTGGGQEIGIRSGTENVPGIYGLGEAVRILNKDLDAVISKVDNLKNILKNEI
                     IDNIEDIKINSPEDGVCHILNVSFRGTKGEVLLHYLEQKGIYVSTGSACSSKKKGSYV
                     LNAIGLTNEEINGTIRFSLSDMNTEEEMLEAVKVLKESICDLRSIMKRK"
     gene            complement(5268..5990)
                     /locus_tag="PC471_003290"
     CDS             complement(5268..5990)
                     /locus_tag="PC471_003290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004398740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HDF2790394.1"
                     /translation="MITIKNLSKSFGDLNVLKNIDLEIAKGEIMVIVGPSGSGKSTLL
                     RCMNLLEIPTGGEIIFEGKNLVDKKTNIDEVRQNIGMVFQNFNLFPHKTILDNITLAP
                     IKLKKMTKEEAEKKAEILLSRVGLLDKKDSYPSQLSGGQKQRIAIARALAMEPDMMLF
                     DEPTSALDPEMVNEVLDVIKELAKEGMTMAIVTHEMGFAKEVADRVIFIDGGSILEDN
                     TPEEVFGNPKHERTKAFLAKVL"
     gene            complement(6049..6726)
                     /locus_tag="PC471_003291"
     CDS             complement(6049..6726)
                     /locus_tag="PC471_003291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HDF2790395.1"
                     /translation="MSLDFSFLSRFGTSFLEGTGVTVSISLVALCFGFIIGIIICMAK
                     ISKSKVLRAISSIYIEVLRGTPLLVQIYIVWFGLPQLGIRFPMLFGIPSEFIASAFAL
                     SVNSGAYVAEILRSGIQSVDNGQMEASRSLGLNYWSTMRYIIIPQAIKNILPSLANEF
                     ITLVKESSIISVIGVVEIMRTADIVKNAAFRALEPLIVAAAIYFVITFTLSRLVGLLE
                     KKLSVSN"
     gene            complement(6754..7569)
                     /locus_tag="PC471_003292"
     CDS             complement(6754..7569)
                     /locus_tag="PC471_003292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HDF2790396.1"
                     /translation="MKVFKKLLSLGLVLGLTLSLVGCSGGGEKTKLEQIKDNGKLVVG
                     TSAEFPPFEFHKVVDGKDSIKGFDIMLAEEFAKELGVKVEIKDMSFDGLIGALNADQV
                     DIVLAGMSPTPEREKSVDFSELYYLSRNAVIVKDADIDKVKTEDDLKKLRVGVQAGSI
                     QEEYVVNTLKMTTTKSLKAIPDLITELKNGNIDAVVTNEAVSLINVKKYDGIKMANTE
                     VGKDVTEGMAAAIKKSDNNKDFIELLNKKIKELQDGKKIEEFLNEASTEAASN"
     misc_binding    complement(7795..8005)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
BASE COUNT         2563 a         1541 c          794 g         3275 t
ORIGIN      
        1 attataaaaa aaaagaaccc tttttataac taagggttac tttttttctc tattttttct
       61 tattatatca ctattttttc cctatttcaa tattttgaca ttttttccat tatactgaaa
      121 agatatctta aatttttaac ttggataaat tttaaagcat atacttaaaa ttaacatact
      181 tgttttctat tttattacac ttaatcctta tttttatcat tctacaaaac aaaaataaaa
      241 aagaataaga taataaataa aatacttatc accttattct ttatttaatt attttacaac
      301 gaaaaatcta aactactttt taaatagctt cctcttccat caatttattt gtatattact
      361 gatttaattc ttcaagtaac tgattggttc ttgcaatact ttatcctaaa ctctggaaac
      421 tttgtaaact acctctctag catttggtaa atttccatct ggaataccta cagcaatacc
      481 aaatggtaca gaataccctt ctggtatatc aagtagttct ctgtattttt tatttgtatc
      541 aaacttcatc tttattgact ctgattcttt tacatcttct ttatccaata cactcacaag
      601 accttcaata tccccttgtt caacaggtgt aaataaacct ctaaccattc ctattataca
      661 acttccaagc cctagtgctt gtgcagctgt tgttatattc cctgtaagta ttcctatatc
      721 atatggagcc catgaactac tttcatcata agaaataaat aaaacggttg gagcaccata
      781 aaataactta aagtttttat ttatctcaac attactcacc tcatgaatcc tagataaagt
      841 atcatcacta atctctttta aaagtttctg gtcttgcaca acagttatgt gccaaggctg
      901 tttattacat cctgaaggtg ctgcaaatgc acagtgtaat aatgtgttta cttgctcatc
      961 actaatttgt tctgtcgtaa attttcgtat acttctacga ctttgtatta aatttattgt
     1021 ttggttgtct tgaaaattat tattcatatc tattcccctt tttatgtatt tcttagtaca
     1081 aatactaaat cttcagaaaa ttaatcttat aattaaattt ctttacaata ttattataat
     1141 tatttttagc ttgtatgtga agacttaaaa atataaacac ttttaatata tctcaattat
     1201 atatataaat gaaaatctgg tattcgtcat ctatagaaac tgctcttttc tatagtttaa
     1261 tcaataccag attaataaaa catgatatat taatttaaag gaccccatcc ctctttagaa
     1321 tgaatctctg tttttacaga cttaacacta tctgtgtcaa catcccatga cctacctgca
     1381 acagaaatac catttttacc tttataccat ctaccttcat cataatcgta aactagggaa
     1441 gtataccaat tacccccagt cttattattt gcaaatacaa ctacagtagc atcagattta
     1501 gtaggattat atgcatatgg ttgtatatat agtgtatcct tacttaatcc atattttgcc
     1561 ttaaattcat ctggaattgt aggccaccca tttttatatt tattgtctaa taaatatttt
     1621 ctaaaatcat cattaccctt tgaggctcct ggtattatag aagggtcttg tgcaaatgat
     1681 gcaatcaaat ctttcatact ttgatgtata tccctagcca tttcaacacc atctggatgt
     1741 tttgtacaag taacatacac ttttgcaatg ctttctattc cagttattcc atcataacca
     1801 tcatcaaacg ttgctgaata tattccacct gacttacact ttggttctgt ttcaaagtac
     1861 ttaccatctt tgttttctaa tacctctttc atgacttcat tcttgccttt acttgattcc
     1921 tcagccattg caataacaat ttgagtcttt atattttctc tattagaaag acatgtaact
     1981 gctttacttt tttctatatt tctaaacaat gctggaacag ctactataac taaaatgcct
     2041 attatagata taactactaa caattcaatt agtgtaaaac cctttttatt cataataatc
     2101 attcccatta aataaaatta tagtacttat ttttaaatac tacatattaa taaattgtct
     2161 acataaatcc tttcaacaga actactaata tacttacaga actactaata tactcaatat
     2221 tcaatttagt aattaaataa cttgtatgct aaaactcaat atcctctacc tctattttgt
     2281 caatagcatc ctgtactaat ttttcaatgt caagtgctgt ttcagatttt tctattttct
     2341 ctaatctagg tttagtgacg ggctttctag gagaaaatac actacaacaa tcttcttcag
     2401 gcactataga tgtttcaaaa gtccctattt ttttggctat atcaactata tctgatttat
     2461 ccattgctat aagtggtcta aacactggta agtctacaac ggcattagta caagtaagtc
     2521 cttgtatagt ttgagatgca acctgcccaa tactttcacc tgtaattaaa gcatcacaat
     2581 gtcttttttc agaaagtctc tgcgctatcc tcatcataaa tcttcttgaa agaatagtag
     2641 attcttcttc attacaattc tcgccaattg ctttttgtat ttctaagata ttaactttat
     2701 gaagccttac tctaccacag tattttgcca gtattttagc taaatcctta actttttcct
     2761 gtgacctttc acttgtaaat ggataactat gaaagtgaac tgcctctact tccatacctc
     2821 tttttgcaac catccatgta gctactgggc tatctatacc tcctgataaa agagacatag
     2881 ctctaccatt cgttccaagt ggcaatccac catatccagg tatagtatca ctatagacca
     2941 tagtatgaaa ttctctgtat tcgcacttta ttttaacttc tggatttctt acatcaacat
     3001 taatcctatc tcctaccttt gataataagt atccacctat atccatactc atttcttgag
     3061 aagtcaatcc gaaagactta tctcctcttc ttgactctac cttaaaagtt ttatatccag
     3121 cctctatttt ttcttctaac atttttaaag ctatttcttt taatgtatca taatcttttt
     3181 tagctcttac tccagggcac acacccacta tcccaaatac ttttctaact tcttcaatta
     3241 cttcttcata atcatagtct tctaaatcaa catatattct tccatactct ttatatacat
     3301 taaatcttcc aattggtttt aacatatttc ttatatttct tataagccta ttctcaaata
     3361 tatatctatt ctttcctttt actccaattt ctccatactt tactattaat atattataca
     3421 ccatttcacc tcttaaacta atttattatc actatttata cagtattctt tattttcttt
     3481 tcattattga ccttaagtca caaattgatt ctttcaaaac tttcacagct tctaacattt
     3541 cttcttctgt attcatatca gataaactaa atcttattgt tccatttatt tcttcatttg
     3601 taagacctat agcatttaat acgtaactac ctttcttttt agaagaacat gcagagccag
     3661 ttgatacata aatacctttt tgctctaagt aatgaagtaa aacttctcct tttgtccctc
     3721 taaatgatac atttaatata tgacatactc cgtcttctgg agaattgatt tttatatctt
     3781 ctatattatc aattatttca ttttttaata tattttttaa gttatctact ttcgaaataa
     3841 cagcatctaa atctttgttt agtattctaa cagcttctcc aagaccatat attccaggta
     3901 cattttctgt tcccgaccta attccaattt cctgaccacc acctgttaac ataggcttaa
     3961 ggcgattatt ttcttttata tacataaatc caattccctt tggaccatga aatttatgtg
     4021 cacttacact cataaaatct atattgtatt tagatggtct aaagttaatt tttccatatg
     4081 actgaatagc atcaacatgg aaataaattt tttcttttaa acccttcaaa tactttccaa
     4141 cttcacctat tggctgtata gttcctattt cattgttaac atgcatcatg cttactaagc
     4201 atgtagttga ctttatcgca ttttttaaat cctctatatt tattttccca tcttttccga
     4261 cctctaaata agttacttca aaaccatctt cttctaaatc ttttaatgta tttaaaacag
     4321 aaggatgttc tatgttagta gatattatgt gattttttct ttttttattt aaactagtaa
     4381 tacctcttat tatagtatta ttacactctg taccacctga tgtaaaataa atctcctttt
     4441 ctttcgctcc tagagattta gctatatctt gtcttacttc ttttatagct ttttcaactt
     4501 ctacaccttt tctatggaca gaagaaggat ttccatattc cgtattaagt gcataaacca
     4561 ttttatctat aacttcttga taaggttttg tagttgcgct attgtctaag tatatttcca
     4621 acttaattac acctaccttt atttaaaatc ttcaactcta actattatac cactaattat
     4681 agtatttttt ttgttaatct ttattaaaaa gacaatctta aattataaaa tattatttca
     4741 tgaaaagcta gttttttcac tttattacaa atttttatag taaaatataa tatacttata
     4801 ctatacccta caaaaatata ttataaaata caatatatga taaaataata tattatataa
     4861 aataaatata ttatattata catatactta tttttactat aaaagttaaa tttataagct
     4921 tacttgttat aaaaataact tttataattt atttcaaaaa aaatattgat ttttgtgtcg
     4981 cattttgtta taataaatta aggttatccc cctggtaacc ctagttagtt aatatatcta
     5041 ttcccaatac cctttcataa ggatatattt tatatttatt aaaaatcgga ttagtaacgg
     5101 aaattaattc ggtggaaaaa aagagcttac cccgagctct tttttttctg caaaaaatcg
     5161 taaaaataaa gttaatatta atataacttt aattttaaaa ttaatattaa ccatcactaa
     5221 aaaaggatgt attgaatttc taatatcaat aacatccttt ttatatatta tagcactttt
     5281 gctaaaaatg cttttgttct ttcatgtttt ggatttccga aaacttcttc tggagtatta
     5341 tcttcaagta tacttcctcc atctataaaa ataactctat ctgcaacttc cttagcaaat
     5401 cccatctcat gagttacaat tgccattgtc ataccttctt tagcaagttc ttttataaca
     5461 tccaaaactt cgtttaccat ttctgggtca agagcagatg ttggctcatc aaacaacatc
     5521 atatctggtt ccatagcaag tgctctagct attgctattc tttgcttttg tcctccagat
     5581 aattgtgatg gatatgaatc ttttttatct aaaagaccaa ctctactcaa caaaatttct
     5641 gctttttttt ctgcttcttc cttagtcatc ttttttaatt taattggtgc taatgttata
     5701 ttatctaaaa tagtcttatg tgggaataaa ttaaagttct gaaacaccat acctatattt
     5761 tgtctaacct catcaatatt agttttttta tcgaccaaat tttttccttc aaatattatt
     5821 tctccacctg taggtatttc tagtaaattc atacatctaa gaagtgtact tttaccagaa
     5881 ccacttggac ctactataac cattatttcg cctttagcga tttctaagtc tatatttttt
     5941 aatacattta aatctccaaa agatttactt aaatttttta ttgtaatcat attatcactt
     6001 tcctttttac tattctatgt tttccctaaa tgctaaacat gctctcatct agttagacac
     6061 agatagtttc ttttctaata atccaactaa tcttgataga gtaaatgtta taacaaagta
     6121 aattgctgct gctactatta atggctctaa tgctctaaat gctgcatttt ttactatatc
     6181 agcagtacgc attatctcaa caactcctat tacagatatt attgaagatt cttttacaag
     6241 tgtaataaac tcatttgcta atgaaggtaa tatatttttt atagcttgag gtattattat
     6301 atatctcata gttgaccaat aatttaatcc taatgatcta cttgcttcca tttgtccatt
     6361 atcaactgat tgtatacctg atctaagtat ctctgccacg tatgcaccag agtttactga
     6421 taatgcaaat gcactagcta taaattcact tggtatacca aacaacatag ggaatcttat
     6481 tcctaattga ggtaaaccaa accatactat atatatctgt actaataatg gtgtccctct
     6541 taaaacctca atatagattg atgatattgc tcttaatacc ttgctctttg atatttttgc
     6601 catacagata attataccta ttataaatcc aaagcataat gccacaagag aaattgatac
     6661 tgtcacacct gttccttcca aaaaagaagt cccaaatcta cttaaaaaac taaaatctaa
     6721 actcataatt tcatctccca cataactttc ttactaattt gaagcagctt cagtagatgc
     6781 ttcatttaaa aattcttcta tcttcttgcc atcttgtaat tcttttatct ttttgtttaa
     6841 taattctata aaatctttgt tattgtcaga ttttttgatt gctgctgcca taccttctgt
     6901 tacatctttc ccaacttcag tatttgccat ttttattcca tcatattttt taacatttat
     6961 taaagaaact gcttcattag taacaactgc atctatgttt ccatttttta attctgtaat
     7021 taaatcaggt attgctttca aagattttgt agttgtcatt tttaaagtgt taactacata
     7081 ttcttcttgg atacttcctg cttgaactcc aactctaagt ttctttaaat catcttcagt
     7141 ttttactttg tctatatctg catctttaac tataactgca tttctactta aataatataa
     7201 ttctgaaaaa tcaacactct tttctctctc tggtgttgga gacatacctg caagaacaat
     7261 atcaacttgg tctgcattta aagcacctat taatccatca aatgacatat ccttaatttc
     7321 aacctttaca ccaagctctt ttgcaaattc ttcagccaac attatatcga atccttttat
     7381 tgaatctttt ccatctacta ccttatgaaa ttcaaatgga ggaaattctg cactagtacc
     7441 aacaactaat tttccattgt cttttatttg ttctaacttt gttttttctc ctccaccact
     7501 acatcctact agcgataatg tcaatcctaa tactaatcct aaacttaata attttttaaa
     7561 tactttcatt ttaactaccc ctctctttta attataattt ccctcagatt ttaatactta
     7621 aatgtttcga gttacaaaaa aagtctctca gtctttaatg actaagagac gactttaccg
     7681 cgttaccact cttattgata aatacttata ttactttcaa aatttgtgac tgttagcatt
     7741 taagctgtat ggatactttt atttttaaat caaaaaagtc ccttagcttt acagctaaga
     7801 gacgaattat atccgcgtta ccactctttt tgataagtaa gtacttatcc tctcaatccc
     7861 ttaaggcggg taaaccaatt atcatactaa agtttctgat aatctcctcc agagctctct
     7921 tcacatagac ttcattgcta ggcttacacc aacccctaac tcgctgtaaa tccatatatg
     7981 ctactttctc tatcattgga tttgtttcct taaattgtta atacaattgt acttccttat
     8041 aatatttttg tcaataagtt ttttattttt ttaataattt ttacttgtaa attatctaaa
     8101 tactactgtt tatatttatt gatattagtt ttgtaattat aaatagtata tttcatattt
     8161 ttagatattt ctt
//
LOCUS       DAMJYR010000152         8150 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.157, whole genome shotgun sequence.
ACCESSION   DAMJYR010000152 DAMJYR010000000
VERSION     DAMJYR010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8150)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8150)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8150
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.157"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            65..1528
                     /locus_tag="PC471_003293"
     CDS             65..1528
                     /locus_tag="PC471_003293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine:cation symporter family protein"
                     /protein_id="HDF2790397.1"
                     /translation="MTDLVNTINGIVWSPVLVAMCLCIGLYFSLKLKFFQIRDVKEMF
                     TLLLEGDSSHVGISSFQGFATSMAGRIGTGNIAGVAVAIAMGGPGALVWMCIMATLGS
                     ATAFVESTLAQIYKDEHDGQYRGGPPYYFEKGLGWKKCSVLFSIVAIISYTFFMPGTQ
                     SNTLYLAMNQAFGFSKMTIAVASSIALAIIIFGGIKRIGLFAEKVVPIMGGAYLIMTL
                     VIIITNISLVPEAIVTMFKSAFGFGSVFGGMLGSAISWGVKRGIYSNEAGEGSGTYGA
                     AAAEVSHPAKQGLVQAFSVYVDTLLICMATGIMIVITGMYNVTGVCTALPGVEPGPQY
                     VQSAINTLHPQLGDIFIAIVLLLFTFTTLLAFSYMMETNVSYLVTRIKAEKHEKYIIN
                     VCRFMLIACAAMCCFIEPLTSWALGDICIGVLTYINLIAIFTLKKPAIKAYYDYIRQK
                     KMGIKRSDRTFNPIELDIRNAEFWENRYLKEDKHELN"
     gene            1584..2720
                     /locus_tag="PC471_003294"
     CDS             1584..2720
                     /locus_tag="PC471_003294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HDF2790398.1"
                     /translation="MKERIIQVADSKKEKILGLCQSLYDEPEIALQEYKSAKKISEFL
                     REEGFDVEENLAGMATAFKATKKNGDGPKIAFIAEYDALPGNGHACGHHLIASMGVGA
                     GIALSSILDTYKGEVSIIGTPAEETGDGKPYLIEHGVFDGYDAAMMIHPNSKTCVTPE
                     IIAIGGLDFIFTGKASHAGAKPYNGINALDAVVLLYNNINALRQQLVDGTRIHGIILE
                     AGTAANVIPDMGKVRLEIRAKEQNYFDEVVEKVKNCARGAAIATGCELEFYHFEPTCQ
                     GLNENKVLVDIFTKIMEEFGIYEDEQVMLGSTDMGNLSQIMPCIHPLMKFSENGEELH
                     TKEFLEASINSYAKDRVIDGIKILALTGFNLFENPELLKKMKEE"
     gene            2956..4071
                     /locus_tag="PC471_003295"
     CDS             2956..4071
                     /locus_tag="PC471_003295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
                     /protein_id="HDF2790399.1"
                     /translation="METLEVRNIELSGTNYEIGYRLGELVANMPEIIEGQINKSNLVS
                     KKEEKEMIELFDKYCPGLNEELQGFADAIQVNCNQILYYTMTYLKPGCSQVALAPELT
                     ENGHVLFARNFDFSHNMEDFVLCKTKVNGKYAHIGTTIMQFGRGEGMNECGLGVSQSS
                     CGIPVGNSDGLRKPAIVGLQFWAVIRYLLENCKDVDEALEYLKDMPIAYNINLLLADK
                     SGNIALVETLDGKKEVNIINSLESKREYFLHSTNHTHIDKLHKLDPQSMKNSIHRYKL
                     IKEYINKSKKIGEKELMNLLSSKYPNGLSCNYYNDFFGTLKSIVMDLNIGKFNILWGG
                     LENKWESYYLKNDIKSITQRININIEKAPSDFFDFIE"
     gene            4223..5092
                     /locus_tag="PC471_003296"
     CDS             4223..5092
                     /locus_tag="PC471_003296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HDF2790400.1"
                     /translation="MDYLKYFEKAVMYIENNLHENITVNDVAKETGYSYYHLTRLFKS
                     MFGESVGSYIKKRRLVSSTKELLYSDKKVIDIAIGSGFESSEAFSRAFKSIYKVSPIE
                     YRKNRIDVFVGKKKKLELDFMRHLVGNITIKPVIKEIEKIKVIGIKDKVILENNSLPD
                     LWEKFRKVHHIVPNTLPSRRVFGICEAISEIHLVSESMEFNEIIGLEVNSYDVIPNSF
                     VSKTIKEGKYAIFTHTGSLDNLDKTYEYIWGTWFLSSKEELDIRDDFEVYDERFLGPS
                     NVNSQIDIYIPIK"
     gene            5284..6174
                     /locus_tag="PC471_003297"
     CDS             5284..6174
                     /locus_tag="PC471_003297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDF2790401.1"
                     /translation="MAKLTLEHIKGNTYYIPLPTIVGVYVDGKDAILIDSGNNKDTAR
                     QVLRLLEEHNLNPKLIINTHSNADHIGGNAYLKNQTKCKIATTKIEGYFTENPILESA
                     FLYGGYPSKALKNKFLLAKESEVDYIIPSNGKIVDTELEAISLPGHYFEMIGVRTPDN
                     VLFVADSLTPENIITKYHFFFLLDIDSQFKTLEKLRMLEADFFVPSHSVKTTDIKSLI
                     NINKKKMEEIIDNIKKVCYEPVMIDEVIEKMCDLYNVKLDANQYVLVGSTIRSYITYL
                     YENNMVEYIFDGGKMMIKAV"
     gene            6392..8146
                     /locus_tag="PC471_003298"
     CDS             6392..8146
                     /locus_tag="PC471_003298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp23"
                     /protein_id="HDF2790402.1"
                     /translation="MYIMNKKFKKFSKKLSILILVLIMFLFNNLTANAQIKEDSIIGI
                     DRYETAGLIADRQTYDTVILVNGDKNLSDGLSSSGLAGAINAPILLTKKSEIPKATLN
                     RLDNKSLNVVKKVYIIGGYNTIENSVEKDIKRKGIQVERINGNNRIETSYNVAKKINE
                     VSSVKEVMLTNGFVGEADAMSIAPVVAKNKGAIILTDGKSVPFKTESLNVYAIGGKSA
                     INEDLVNETKATRIGGNDRFETNKKVMEKFYNGATDFYITNGYQLVDALTLSPLAKEK
                     PIVLVADGSNKGILKGAKSITKVGGIDANIYKQCLDMIKYNNMDMTPNILKHLTSVEG
                     NEVSEILIESDNLGVVVEKTNTNKFEFDYVSVTNEKNCTFSVYKENSSNNIKNGKLVV
                     SAKKKIDKKDSDLQDMNGDNMINANKDRMVNVIKIGVPDKMYSNFKVDVESGSVELYN
                     IKGGATVDVNDGIANIVDNNVTNPFNINTNSGISGVTAETISSEIKFRANNGIVNVTA
                     TNINGNISLFGSDNKGTFDGIFKLNLKKEPSNLHLKLIGNGINKLPEGWSKDYILGNG
                     QPIIEVKNNGINNISIGE"
BASE COUNT         3230 a          810 c         1500 g         2610 t
ORIGIN      
        1 aaatattgaa atatagctgt ttaaattatt gtctataaat ataaaatgaa tggtggtgag
       61 ttaagtgacc gatttagtta acaccattaa tggaattgtt tggtcacccg tattagttgc
      121 aatgtgtttg tgtataggat tatatttttc attgaaatta aaattttttc aaataagaga
      181 tgtaaaagaa atgtttactc tcttgcttga gggagatagt tctcatgttg gtatatcatc
      241 atttcaaggt tttgctacat ctatggctgg aagaataggg actggaaata tagctggtgt
      301 agctgtagca atagcaatgg gaggtccagg agcattagta tggatgtgta taatggctac
      361 attaggttca gcaactgcat ttgtagaatc tactttagct caaatatata aagatgagca
      421 tgatgggcag tatagaggag gacctccata ttattttgaa aaaggtcttg ggtggaaaaa
      481 gtgttcagtt ttattttcaa tagttgcaat aataagttat acatttttta tgcctggtac
      541 acaatcaaac actttatatc tagcaatgaa tcaagcattt ggattttcaa aaatgacaat
      601 agcagtagca tcttctattg cattagcgat aataattttt ggaggaatta aaaggattgg
      661 tttatttgct gaaaaagtag tacctataat gggtggagca taccttatta tgacattagt
      721 aataattata acaaatatta gtttagttcc agaggctatt gtaacaatgt ttaaatctgc
      781 atttggattt ggttcagttt ttggaggaat gctaggttca gcaatatctt ggggagtaaa
      841 aagaggtata tactcaaatg aagcaggaga aggttctggg acttatggtg cggcagcagc
      901 agaagtaagt catccagcca aacaaggatt agttcaagca ttttctgttt atgtagacac
      961 attattaata tgtatggcta caggtattat gattgtaata acaggaatgt ataatgttac
     1021 aggtgtttgc acagcactac caggtgtaga accgggacca caatatgtac agagtgcaat
     1081 aaatacatta catccacaat taggtgatat ttttatagca atagttttat tattatttac
     1141 atttacaact ttattagcct tctcatatat gatggagact aatgtatctt acttagtaac
     1201 tagaattaaa gcagaaaaac atgaaaagta cataattaat gtctgtagat ttatgcttat
     1261 agcatgtgca gcaatgtgtt gttttataga accacttaca agctgggcat taggtgatat
     1321 atgtataggt gtattaacat atataaattt aattgccatc tttacactaa aaaaaccagc
     1381 tataaaggca tattatgatt atataagaca gaagaagatg ggaataaaaa ggagtgatag
     1441 aacttttaac cctatagagt tagatataag aaatgctgag ttttgggaaa atagatactt
     1501 aaaagaggat aagcatgagt taaattaaag ttataataaa atataaaaat tactaaataa
     1561 atttagtggg aggtaagatt ggtatgaaag aaagaataat acaagtggct gattcaaaga
     1621 aagaaaaaat tctagggcta tgtcaatctt tatatgatga accagaaatt gctttgcaag
     1681 aatataaatc tgctaaaaaa atatcagaat ttctaagaga agagggtttt gatgttgagg
     1741 aaaacttggc aggtatggct acagctttta aagcaaccaa gaaaaatgga gatggtccta
     1801 aaatagcatt tatagcagaa tatgatgctc ttccaggcaa tggacatgca tgtggacatc
     1861 atttaattgc atctatggga gttggagctg gaatagctct ttcaagtata ctggatactt
     1921 acaagggaga agtaagtata attggaacac ctgctgaaga aactggagat ggaaagcctt
     1981 acttgataga acatggtgta tttgatggct atgatgcagc aatgatgata catccaaatt
     2041 caaaaacttg tgtaacacct gaaattatag caataggagg tctagatttc atatttactg
     2101 gaaaagcatc tcatgcagga gctaagccat ataacggaat aaatgcttta gatgcagtag
     2161 tactactata taataatata aatgcactaa gacaacaatt agtagatgga acgagaatac
     2221 atggtattat attagaagca ggaactgctg caaatgtaat acctgatatg ggaaaagtaa
     2281 gattagagat aagggcaaaa gaacaaaatt actttgatga agtagtagaa aaagtgaaaa
     2341 attgtgctag aggtgctgca atagccactg gatgtgaact tgaattttat cactttgaac
     2401 caacttgcca aggattaaat gaaaataaag ttttggtaga catatttaca aagattatgg
     2461 aggaatttgg aatatatgaa gatgaacaag taatgttagg ttcaacagat atgggaaatt
     2521 taagtcaaat aatgccatgt atacatcctt tgatgaagtt ttctgaaaat ggagaagaac
     2581 tacatacaaa agaatttttg gaggcttcta taaattcata tgcaaaagat agagtaattg
     2641 atggtataaa aatattagct ttaacaggat ttaatttatt tgaaaatcca gaattattga
     2701 aaaaaatgaa ggaagagtag aacttagact atttttaatt atactcttta aaataagaag
     2761 agtgtctcaa aatgaacttt ttagtttatg agacactctt ttttgtatga aattaaatat
     2821 atttttatta ttttaaacat gaaaaagagg cttatctgtt tttatatgac cttttttaac
     2881 ttgtagcaca aaatatcatg aatgattaca attcgaatga taaaatcgat tataagatat
     2941 ataggagggg tacatatgga aactttagaa gtaagaaata ttgaattgag tggtacaaat
     3001 tatgagatag gatacagact aggagaattg gttgctaata tgccagagat aatagaaggt
     3061 cagattaata aatctaatct ggtaagcaaa aaagaagaaa aagagatgat agagttgttt
     3121 gataaatatt gtccaggtct taatgaagaa ttgcaaggtt ttgctgatgc aattcaagtg
     3181 aattgtaatc aaattttata ttatactatg acttatctta aaccaggttg tagtcaagtt
     3241 gcattagcac cagaattaac tgaaaatggg catgtactat ttgcacgtaa tttcgatttc
     3301 tcacacaata tggaggattt tgttttatgt aaaactaagg taaatggaaa atatgcacat
     3361 ataggtacta ccattatgca atttggaaga ggtgaaggta tgaatgaatg tggcttaggt
     3421 gtaagtcagt catcatgtgg tattcctgta ggcaattcag atggtttaag aaaaccagca
     3481 attgtaggtt tacagttttg ggctgttatt agatatttgt tagaaaattg caaagatgta
     3541 gatgaagcat tagaatattt aaaagatatg ccaattgcat acaatattaa tttattgctt
     3601 gcagataagt ctggaaacat tgcacttgtg gagactttag atgggaaaaa agaagtaaat
     3661 ataatcaaca gtttagaaag taaaagagaa tattttttac actctactaa tcatacacat
     3721 attgataaac ttcataaatt agatccacaa tcaatgaaaa attctattca tagatataaa
     3781 ttaattaaag aatatataaa taagtctaaa aaaattggag agaaagagtt aatgaattta
     3841 ttgtcttcaa aatatccaaa tggcttatct tgtaattact ataatgattt ttttggaact
     3901 ttaaaaagta tagttatgga tttaaatata ggaaagttta atattttatg gggtggacta
     3961 gagaataaat gggagagcta ctatttaaaa aatgacatta aatctataac acagaggata
     4021 aacataaata ttgaaaaagc accatctgac ttttttgatt tcattgaata aaaaatgtat
     4081 ttaattattc taaataatta ggacatataa atcaactaaa agtttttatt tatcgtattg
     4141 aattagttat aatattttag tatagtaaaa aatagtgcta ttttacatat ttaaagagtt
     4201 taagtagaag agaggtgcca tattggatta cttaaagtat tttgaaaaag ctgtaatgta
     4261 tattgagaat aatttacatg aaaatataac tgtaaatgat gtggcaaaag aaactggata
     4321 ttcttattat catttaacac gcttatttaa gtctatgttt ggtgaaagtg taggaagtta
     4381 tataaaaaaa agacgattag taagtagtac aaaagaactt ttatatagtg ataaaaaagt
     4441 gatagatata gctattggta gtggatttga atcttcagaa gcatttagca gagcatttaa
     4501 atctatatac aaggttagcc ctatagaata tagaaaaaat agaattgatg tatttgttgg
     4561 taaaaagaaa aaattagagt tagattttat gagacatctt gttggaaata taacgattaa
     4621 accagttata aaagaaatag aaaaaataaa ggtaataggt ataaaggata aagtaatttt
     4681 agaaaataat tcattacctg acttgtggga gaaattcaga aaagtacatc atattgtccc
     4741 aaatactttg ccatcaagga gagtttttgg aatctgtgaa gcaatctctg aaatacacct
     4801 agttagtgaa agtatggagt ttaatgagat aataggactt gaagttaata gttatgatgt
     4861 gattccaaat tcatttgttt caaaaactat taaggaagga aagtatgcta tatttactca
     4921 tactggtagc ttagataatt tagacaaaac ttatgaatat atatggggta cttggttttt
     4981 aagttcaaaa gaagaacttg atataagaga tgattttgaa gtatatgatg aacgattttt
     5041 aggtcctagt aatgtgaact cacaaattga tatttatata cctataaaat aatctataag
     5101 aaaactaaaa gataatttga ttaagaaata gatgttcgta aaagagcatc tattttttat
     5161 ttttataaga tttagataat tttaataata aaagaaataa aaatatatta taaaaacaat
     5221 ttgattggaa ttttcagaaa attaatgcta taattaaaag taaatttaaa gggaggttat
     5281 aaaatggcta aattaactct agaacatata aaaggaaata catattatat tccactccct
     5341 acaatagtag gtgtatacgt agatggtaag gatgcaattt taattgacag tggaaataat
     5401 aaggatacag ctagacaagt tttaagatta ttagaagaac ataatttaaa tcctaaatta
     5461 ataataaaca ctcattcaaa tgcagaccat ataggaggaa atgcatattt gaagaatcaa
     5521 acaaaatgca agattgctac gactaaaatt gaaggatatt ttacagaaaa tccaatatta
     5581 gaatcagcat ttttatatgg tggctatcca agtaaagcac tgaagaataa atttttatta
     5641 gcaaaggaaa gtgaagttga ttatattatc ccttcaaatg gcaaaattgt agatacagaa
     5701 cttgaagcga tttctcttcc aggtcattat tttgaaatga ttggtgttag aactccagat
     5761 aatgttttgt ttgttgcaga tagtcttaca ccagagaata taattactaa atatcatttt
     5821 ttcttcctac tggatataga ttcacagttt aagacactag aaaaattgag aatgttagag
     5881 gctgatttct ttgttccaag tcactctgtc aaaactacag atataaaaag tttaattaat
     5941 attaataaga agaagatgga agaaataata gataatatta aaaaggtttg ttatgagcca
     6001 gttatgattg atgaagttat tgaaaaaatg tgtgatttgt acaatgtcaa gcttgatgct
     6061 aatcaatatg tgttggttgg aagtacaata cgttcttata taacttattt atatgagaat
     6121 aatatggttg aatatatatt tgatggtggt aagatgatga taaaagctgt gtaaatagta
     6181 ataattttca aattatatga tgaaattgtt ttagtatgac tgttatttga aaagtaatat
     6241 aaaaaaattt atataaataa gctaagatga ttgatataaa gttactgata attttaaggt
     6301 aatatatacg ttactataat attatgttat tatttgtaaa taaggggata atttataagc
     6361 aaattaaaga ccttaattta caggaggaat agtgtatatt atgaataaaa aattcaaaaa
     6421 attcagtaaa aaactaagta tattaatttt agttttgatt atgtttcttt ttaataattt
     6481 aactgcaaat gcacagatta aagaagattc aataatagga attgatagat atgaaacagc
     6541 aggacttata gcagatagac aaacttatga tactgtaata ttagttaatg gagataaaaa
     6601 tttatctgat gggctttcat caagtggatt agctggagct attaacgcac caatactttt
     6661 gactaaaaaa agtgaaatac caaaagctac tttaaataga ctagataata agagtttaaa
     6721 tgttgtaaaa aaagtatata taattggtgg ttataatact attgaaaatt cagtagaaaa
     6781 agatataaaa agaaaaggta ttcaagtgga aagaataaat ggtaacaata gaattgaaac
     6841 tagttataat gtagctaaaa aaataaatga agtaagtagt gtaaaagagg taatgcttac
     6901 aaatggattt gtaggagagg ctgatgctat gagtatagca cctgtagttg caaaaaataa
     6961 aggagctata atacttacag atgggaaatc agttccattt aagactgaaa gtttaaatgt
     7021 ttacgctata ggtggcaaat ctgcgataaa tgaggattta gtaaatgaaa ctaaagcaac
     7081 tagaatcgga ggaaatgata gatttgagac caataaaaag gtaatggaaa agttttataa
     7141 tggagcgaca gatttttata taacaaatgg atatcaatta gtagatgcac taacattatc
     7201 tcctttagcc aaagaaaaac caatcgtttt agttgctgat ggaagtaata aaggtatttt
     7261 aaaaggagct aaaagtataa caaaagttgg aggtattgat gccaatatat ataaacagtg
     7321 tttagatatg attaaatata ataatatgga tatgactcca aatattttaa aacacttaac
     7381 ttcagttgaa ggaaatgaag tttctgaaat acttatagaa tcagataatt taggtgtggt
     7441 tgttgaaaag acgaatacca ataaatttga gtttgattac gtaagtgtaa ctaatgaaaa
     7501 aaattgtact ttttctgtat ataaagaaaa ttcttctaat aatataaaaa atggaaagct
     7561 agttgtaagt gctaagaaaa aaatagataa gaaagatagt gatttgcaag atatgaatgg
     7621 cgacaatatg ataaatgcaa ataaagatag gatggtaaat gtgataaaaa ttggagttcc
     7681 cgataaaatg tattctaatt ttaaagtaga tgttgaaagt ggttcagtag aactttataa
     7741 tattaaagga ggagctactg ttgatgtcaa tgacgggatt gctaacattg ttgataacaa
     7801 tgtaacaaat cctttcaata taaatactaa tagtgggatt tcaggtgtaa cagctgaaac
     7861 tatatcatct gaaataaaat ttagagcaaa caatggaatt gtaaatgtaa cagctacgaa
     7921 tataaatggc aatataagtt tatttggaag cgataataaa ggtacctttg atggaatatt
     7981 taaattaaat ctgaaaaaag aaccatcaaa tttacacttg aagttaattg gaaatgggat
     8041 aaataaattg ccagaaggtt ggtctaaaga ttatatttta ggaaatggac aaccaattat
     8101 tgaagttaaa aataatggca taaataatat atcaattgga gaataaaaac
//
LOCUS       DAMJYR010000153         8133 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.158, whole genome shotgun sequence.
ACCESSION   DAMJYR010000153 DAMJYR010000000
VERSION     DAMJYR010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8133)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8133)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8133
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.158"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            112..822
                     /locus_tag="PC471_003299"
     CDS             112..822
                     /locus_tag="PC471_003299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2790403.1"
                     /translation="MINIGICDDELHYRIKIKDILSEILSSYPINYNIYEFSAGEELL
                     NNYPKDLDILIMDIQMKTINGMDTARKIREFDHKLEIIFVTSFVEFMQEGYEVKAYRY
                     ILKPINKEKISKSVLPCINEMMKKRNNYLTINVKNYVDRIKIDSITYIETDRPNILIY
                     THDDMYITKMSISKIEKILNEYGFFRCHNSYIVNLKLVESMSGSTVIVDGKSIPISKY
                     RVKGLKLAITNILGDIVC"
     gene            816..2132
                     /locus_tag="PC471_003300"
     CDS             816..2132
                     /locus_tag="PC471_003300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HDF2790404.1"
                     /translation="MLTNLSVGFWNIINLVSGTIDWIIFFLVIHLVGKRKISNIRYIF
                     TTIIIIIFMAYINVVKVFPNIKILLCMVIGISFYITSYEDKIYKSIIVSLLFWLGLMV
                     AEGSAVGLVVFINKLNNINITQHENLFRIQAIIMSKVFLFIGFILFKYFKLSLDFKPK
                     DMILIGIPILSNIVSLLLIFEYNLRVNTITTENLFIFIFAILLILSSSIILLIVIGKI
                     VQDDKLNLEYELINERIKTNYRNYENINEVHDRLKYVYHDLKNHMICIKNYDTKEEII
                     SYINNLEFQISEFEHFKNTGNKTLDLILAEKISICKKYDIQIEDNINISKLNFIKNND
                     ICSIFANSLDNAIEACMDINNEIEKRIEVKATYINKFAIIKFINTKVNDIKLIDKRIQ
                     TSKDDDKIHGIGLASIKYIVNKYGGETIVNYSDNEFILKIMIPIKS"
     gene            2144..2284
                     /locus_tag="PC471_003301"
     CDS             2144..2284
                     /locus_tag="PC471_003301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic lactone autoinducer peptide"
                     /protein_id="HDF2790405.1"
                     /translation="MKKIALNLLKNISALSFGIAVLSANSASSWVAHQAKEPQALQKL
                     KK"
     gene            2343..2924
                     /locus_tag="PC471_003302"
     CDS             2343..2924
                     /locus_tag="PC471_003302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HDF2790406.1"
                     /translation="MFKRLSYKFANILVNNEIVESEDFEIYRYGFETLIYFIVNILVA
                     LFIGIIFDRFIHTVIFLSCYCTLRQFTGGYHARNYKECTLTFAVIYLITIFSANNIDI
                     NKYKYLLVLLMIISILTIYKLAPLEHRNKPLSESEKKHYRKTVQKILFVIICLIILCK
                     ILNIFQQYVIYAIISIYWIAILIYIGMKVNSDQ"
     gene            3071..4780
                     /locus_tag="PC471_003303"
     CDS             3071..4780
                     /locus_tag="PC471_003303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2790407.1"
                     /translation="MSSIKAISDFITKIIRKNILFAITIIIINYHLIQRWTHISNRGI
                     SFNDYLSLSVYTCMTISILTMLNVIFYMRKSYDTVKILCRNNFDYFLGISVAVTRNNF
                     IFCCIPIIYAIAYSSLIRTLSIVTIKSTIFILIFEWFLPLTILSIVISFISVIINNLI
                     LKITLSSVFLYLTSTNMLDRVIDIKEPFFKALFKSLNIFEDQSYAYYSDFVGKVNNSA
                     YVIDKIIPTLFAITLIIVAYLILNDIRKKQIKSLIVIVVYSILFIGLNYISIDMSTYR
                     DEERSYGEYLKKSNISDIKIKSHDMKIDFKSKSNIKDRIVLLNTSNEELTNINMILDE
                     IFDIKYIKINSENIKFNKKKDLIEVNLKESLEPNDEVTLDIEYEGYINVLTSWNQNKY
                     IATKDDIMLPPGSLAWYPKVNQSSCMAYKINLSSKNNVYSNLPLLNKTSNFLKKDYIF
                     EGKSNDIAIYAGEFKEEVKDDIKIIYPSDSNVNMDIEEKIKFLIETRNEILGDSKASV
                     DAKKYVDEVDNVMESIKRKKINMILVADLPSNEFLYEDSKGEKISITIYHWLSGNILI
                     IDK"
     gene            4795..5667
                     /locus_tag="PC471_003304"
     CDS             4795..5667
                     /locus_tag="PC471_003304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDF2790408.1"
                     /translation="MINKLEIRNLNFNYKNKKALDNINLTLIDGVVALLGPNGAGKST
                     LMRLLVTLYETSIGEIELNNIKYSKNNEKIKANVGYVPQDFDMYNNINGQEYLEFVAK
                     MRGVSKNDLKKHIQKVVSKVNLDKFINKKIGTYSGGVKRRLGIAQALIGDSKLIVMDE
                     PTVGLDPEQRNEFRRLLPEISKNSIVLISTHIVEDIQFNCDKLIILNQGKILYDGTIN
                     KFIDMVEVYSVVVSNDEFEYLERNIDIIDYRRVKDGVQVKYIGKNTEKLKDSLRIDTT
                     LQDAYINFLHKELK"
     gene            5668..6456
                     /locus_tag="PC471_003305"
     CDS             5668..6456
                     /locus_tag="PC471_003305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2790409.1"
                     /translation="MCILELIKFELKKIELKHVLIFVIISILCIFITGILDYNELIFY
                     NDVPIMDTYSIAGWLGIIKISDVFIPIMSAITIIYVFSKDYSGNINEILTLYNKKKYN
                     QFIVFRWCVILAIYTVLLLISITIVYTFTSVKGFNIDKIDFVYSVKPIDVFLKTFPTL
                     LWYTTFPLMILTLTKNRFTTIAILITYTFADIFFILHMYPFVSMINVNGFYIQKMFYM
                     PNNIDVRFNELKNYFFINRGSLVVISIIIVSYIARKSTVLKKSK"
     gene            complement(6552..7385)
                     /locus_tag="PC471_003306"
     CDS             complement(6552..7385)
                     /locus_tag="PC471_003306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase"
                     /protein_id="HDF2790410.1"
                     /translation="MNVENQSLSDIEIILSHRYDNGADYWTTPDKRLIKGSPFSTLDS
                     VLYLLELGVNPDDTLLKECADLIFSTWREDGRFKLYPNGGIYPCHTANAANVLCHMGY
                     ASDIRMQKTFQHFLDTQYTDGGWRCNKFSFGRGPETEYSNPLPTLNILNAFRFSNYLN
                     KESKLDKAVDFLLDHWTIKKPIGPCHYGIGTLFMQVEYPFRNYNLFLYVYVLSFYNCA
                     KKDNRFLEALKILESKMIGNKIVVERVVPKLSKLSFCKKGESSEIATTHYYEILKNLE
                     K"
     gene            complement(7453..8082)
                     /locus_tag="PC471_003307"
     CDS             complement(7453..8082)
                     /locus_tag="PC471_003307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3786 domain-containing protein"
                     /protein_id="HDF2790411.1"
                     /translation="MDEKITKNNYNVPFDYVRKKLKELDPHTISNLSNIPFNNDLNRF
                     TLNFFDRQYIIDYPSGEVWNSNGSLCNNFELKILIVRYLINAKGIPRTNKYVTFKEIP
                     GGNVYYPNFLNRTLYRLSEVFTSQIDKYKLVMEKIGAEKVNMGDLAYRFTYMGNTSMV
                     FILWFGDDEFPPNSNILFDSNIVYYFNAEDLAVVPDTAINAILNKINQL"
BASE COUNT         3280 a          793 c         1150 g         2910 t
ORIGIN      
        1 aaaattaaaa tctaaatagc tatttactac agaagtatgt taaaatatgt aaaatgaata
       61 gaaatttata tatttttatt aaaaatatat aagaaaggat gtttaagaat tgtgattaat
      121 ataggaatat gtgatgatga actacattat cgaataaaaa taaaagatat attaagtgaa
      181 atattaagtt catatcctat aaattataac atatatgaat tttcagcagg agaagagtta
      241 ctaaataact atcctaaaga tttagacata ttaattatgg acatacaaat gaaaacaata
      301 aatggtatgg atactgctag gaagataaga gagtttgatc ataagttaga aataatattt
      361 gttacttcat ttgtagagtt tatgcaagaa ggttatgagg taaaagcata cagatatata
      421 ctaaagccaa taaataaaga aaaaatttct aaaagtgtat taccttgtat aaatgagatg
      481 atgaaaaaga gaaataatta cttaactata aatgtaaaaa attatgtaga ccgaattaaa
      541 atagattcta ttacttatat agaaacagat agaccgaata tattgattta tacacatgat
      601 gatatgtata ttactaaaat gagtatatct aaaatagaaa agatattaaa tgaatatgga
      661 ttttttagat gtcataatag ctatatagtt aacttaaaat tagtagaatc aatgagtgga
      721 agtactgtta tagtagatgg aaaaagtatt ccaataagta aatatagggt taaaggcttg
      781 aagcttgcga taactaatat attaggggac atagtatgct aacaaattta agtgtaggat
      841 tttggaatat tattaattta gtttcaggta ctatagattg gattatattt tttttagtaa
      901 tccacttagt tggaaagagg aaaatatcta atataagata tatatttaca acaataatta
      961 taattatatt tatggcatac ataaatgtag ttaaagtgtt tccaaatatt aagattcttc
     1021 tatgtatggt aataggaata tcattttata taacatcata tgaagataaa atctataaaa
     1081 gtataatagt tagtttattg ttttggctag gattaatggt tgctgaaggt tctgcagtag
     1141 gtctagtagt atttataaat aaattaaata atattaacat aacacaacat gaaaacttat
     1201 ttagaataca agctattatt atgtcaaagg tatttttatt tataggtttt atattgttta
     1261 agtattttaa attatcttta gattttaaac ctaaagatat gatactaata ggaataccta
     1321 tattatctaa tatagttagc ttattactta tatttgaata taatttaaga gtcaatacaa
     1381 ttactacaga aaacttattt atatttatat ttgcaatttt attgatttta tcatcaagta
     1441 taatactttt aatagtaata gggaaaattg tacaagacga taaattaaat ctagagtatg
     1501 aacttataaa tgaaagaata aaaacaaact acaggaatta tgaaaatata aacgaagttc
     1561 atgatagatt aaaatatgtt tatcatgact tgaaaaacca tatgatttgt ataaaaaatt
     1621 atgacactaa agaagaaatt atatcatata taaataactt agagtttcag ataagtgaat
     1681 ttgaacattt taaaaatact ggaaataaaa cattagattt aattttagca gaaaaaataa
     1741 gtatttgtaa aaagtatgat attcaaattg aagataacat aaatatatct aaattaaact
     1801 ttattaaaaa taatgatata tgttcaatat ttgcaaattc actagataat gcaattgaag
     1861 catgtatgga tataaataat gagatagaaa aaagaataga agttaaagct acatatataa
     1921 ataaatttgc aataataaaa tttataaata ctaaggtaaa tgatattaaa cttattgata
     1981 aacgtattca aactagtaag gatgatgata agatacatgg aataggactt gctagtatta
     2041 aatatatagt aaataaatat ggtggtgaaa caatagttaa ttactcagat aatgagttta
     2101 tattaaaaat aatgatacca ataaaaagtt aggagagtac aaaatgaaaa aaatagcgtt
     2161 aaacttactt aaaaacatct cagcattaag ttttgggata gctgtattat cagcaaacag
     2221 tgcaagttct tgggtagcac atcaggcaaa agagccacaa gctttacaaa aactaaaaaa
     2281 ataacattaa taaaaggacc tcttcttatt aaaggagagg ttttacatgt tgaggaggaa
     2341 atatgtttaa aagattatca tataaatttg ccaatatatt agttaataat gagattgtag
     2401 aaagtgaaga ttttgaaata tatagatatg gatttgaaac tttgatttat ttcatagtta
     2461 atattttggt agcattattt ataggaatta tttttgatag atttatacat acagttatat
     2521 ttttaagttg ttattgcact ttaagacagt tcacaggagg ttatcatgct aggaattata
     2581 aagagtgtac attaacattt gcagttattt atttgataac tattttttca gctaataata
     2641 tagatataaa taaatataag tatttattag tattattaat gattattagt atactaacaa
     2701 tttacaagtt agctccttta gaacatagaa acaaacctct tagtgaaagt gagaaaaagc
     2761 attatagaaa gactgtgcaa aaaatactat tcgtaataat atgtttaatt attttatgta
     2821 aaatacttaa tatttttcag caatatgtga tttatgcaat aatttctata tattggattg
     2881 ctatattaat atatattgga atgaaggtta atagtgatca gtaagattat aaaacatttt
     2941 ctaaattaat taacttcact aattaatatt aactttaaaa aaataatgtc aaggtaagta
     3001 tttgaactta ttaaatatag ttgaatctat tgtaggttgt atcaaaagac tggctaggag
     3061 gggcataaat atgagtagta ttaaggctat atcagacttt ataaccaaaa taattagaaa
     3121 aaatatttta tttgcaataa caattattat tataaattac catctcatac aacgatggac
     3181 acatatatcc aatagaggaa tttcatttaa tgattactta tccttgagtg tatatacttg
     3241 tatgacaatt agtattttaa ccatgttaaa tgtaattttt tatatgagaa aaagttatga
     3301 tactgtaaaa atattatgta gaaataattt tgattatttt ttaggtattt ctgttgctgt
     3361 aactagaaac aactttatat tttgttgcat accaattata tatgctatag catactcaag
     3421 tttaataaga acattgtcaa ttgtaacgat taaatcaact atatttatat tgatatttga
     3481 atggttttta ccacttacta ttttatctat agtgatatca tttatttcag ttattataaa
     3541 taatttaata ctaaagatta ccttatcatc agtattttta tatttaacat caactaatat
     3601 gttggataga gttattgata ttaaagaacc attttttaaa gcattattta aatcacttaa
     3661 catatttgaa gatcaatcat atgcttacta ttctgatttt gtagggaaag ttaataattc
     3721 agcatatgtt atagataaga ttatacctac actatttgca attactttaa taatagtagc
     3781 atatctgata ttaaatgata ttagaaagaa acagattaaa tcattaatag ttatagtagt
     3841 gtacagtatt ttatttattg gattaaatta tatatcaata gatatgtcaa catatagaga
     3901 tgaggagcgt agttatggag agtatttaaa gaaatctaat attagtgata ttaaaataaa
     3961 atcgcatgat atgaagattg actttaaaag caagtcaaat ataaaagata gaattgtttt
     4021 attaaacaca tcaaatgaag agttaacaaa tattaatatg attttagatg aaatatttga
     4081 tataaaatat ataaaaataa attcggaaaa tataaaattt aacaaaaaaa aggatttaat
     4141 tgaagttaat ttaaaggaaa gtttagagcc taatgacgag gtgacattag atatagagta
     4201 tgaaggctat attaatgtgt tgacatcttg gaatcaaaat aaatatatag ctactaaaga
     4261 tgatattatg cttccaccag gttctttagc gtggtaccca aaagtaaatc aatcaagttg
     4321 tatggcttat aaaataaatt taagttctaa aaataatgtc tattcaaatt tacctttact
     4381 gaataagact agtaattttt taaaaaaaga ttatatattt gaaggaaagt caaatgatat
     4441 tgcaatatat gcaggtgagt ttaaagaaga ggtaaaagat gatataaaaa taatctatcc
     4501 atcagatagt aatgtaaata tggatataga ggaaaaaatt aaatttttaa ttgaaacaag
     4561 aaatgaaata ttaggtgata gcaaagcatc tgtagatgct aaaaaatatg tagatgaagt
     4621 agataatgtt atggaatcaa taaaaagaaa aaaaattaat atgatattag ttgctgactt
     4681 acctagcaat gaatttttat atgaagactc aaagggagag aaaattagca taactattta
     4741 tcattggctt tcaggaaata tccttataat agataaataa taaaggaggt taatatgata
     4801 aacaagttag aaataagaaa tctaaatttt aattacaaaa ataaaaaagc tttggataat
     4861 ataaatttaa ccctaataga tggagtagtt gcattattag gtcctaatgg tgctggaaaa
     4921 tcaacattaa tgaggttatt agtgactctt tatgaaactt caattggaga gatagaatta
     4981 aataatataa agtatagcaa aaacaatgag aaaatcaagg caaatgtagg atatgtacct
     5041 caagattttg atatgtataa taatataaat gggcaagaat acttagaatt tgtagctaaa
     5101 atgagaggtg tatctaagaa tgatttaaag aaacatatac aaaaagtagt atcaaaagtt
     5161 aatctggaca agtttataaa taaaaaaata ggtacatact caggtggtgt aaagagacga
     5221 ctaggtatag cacaggcttt aataggtgat tctaagttaa tagttatgga cgaacctaca
     5281 gttggtcttg atccagagca aaggaatgaa tttagaagat tgttaccaga aataagtaaa
     5341 aattctatag tattaatttc aactcacata gtagaagaca tacaatttaa ctgtgacaaa
     5401 ttaattatat taaatcaagg caaaatatta tatgatggta caattaataa gtttattgat
     5461 atggttgagg tgtattcggt agtagtttct aatgatgaat ttgaatattt agaacgtaat
     5521 attgatatta ttgattatag gagagttaaa gatggtgtgc aagtaaagta tataggtaaa
     5581 aatacagaga aacttaaaga tagcttaagg atagatacaa ctttacaaga tgcttatata
     5641 aactttttac ataaggagtt aaaataaatg tgtatattgg agttaataaa atttgagctt
     5701 aaaaaaatag agcttaaaca tgtgttaata tttgtaatta tttctatctt atgcatattt
     5761 attacaggca tattagatta taatgagtta atattttata atgatgtacc tataatggat
     5821 acatattcta tagcaggatg gttaggaatt ataaagatat cagatgtttt tataccaata
     5881 atgtcagcaa tcacaataat ttatgtgttt tcaaaagatt attcgggtaa tataaatgag
     5941 atacttactt tatataataa aaaaaaatat aatcaattta tagtatttag atggtgtgtt
     6001 atattagcta tatatactgt attattatta atttcaatta caatagtata tacatttacg
     6061 tcagtgaaag gttttaacat agacaaaata gattttgttt atagtgtaaa gccaatagat
     6121 gtgtttttaa agacatttcc tacattatta tggtatacaa catttccact aatgatatta
     6181 acattaacaa aaaatagatt tacaacaata gcaatattaa ttacatatac atttgctgat
     6241 atatttttta ttttacatat gtacccattt gttagtatga ttaatgtaaa tggattttat
     6301 attcaaaaga tgttttatat gcctaacaat atagatgtta gatttaatga attaaagaat
     6361 tatttcttta taaatagagg ttctctagta gttatttcta taataatagt ttcttatata
     6421 gcaagaaaat caacggtttt aaaaaagagt aaatagttta atatattttt tataatgtta
     6481 tatttatata gaatagtgac tttaaaaaat ttaaagccac tattttttaa tatgttttat
     6541 ctatataact attatttttc taggtttttt aatatttcgt aatagtgtgt tgtggctatt
     6601 tcacttgact cgcctttttt acaaaaagat aatttagata gttttggaac tacacgttca
     6661 acgacaattt tattacctat cattttagat tcaagtatct tcaatgcttc taaaaatcta
     6721 ttatcctttt ttgcacaatt ataaaatgat aaaacgtaga cataaaggaa taggttataa
     6781 ttacggaaag gatattcaac ttgcataaat aaggttccaa ttccatagtg acaagggccg
     6841 attggttttt taatagtcca atggtcaagt agaaaatcta cagctttgtc aagttttgat
     6901 tctttattta gataattact aaaacggaat gcatttaata tattaagagt tggtagtgga
     6961 tttgagtatt cagtttctgg acctcgacca aaactaaatt tattgcaacg ccacccacca
     7021 tcagtatact gtgtatctaa aaaatgttga aacgtttttt gcattctaat gtcagatgca
     7081 tatcccatat gacagagtac gttagcagca tttgcagtat gacaagggta aatacctcca
     7141 tttggatata gtttgaaacg tccatcttct cgccatgtgc taaatatcaa gtcagcacat
     7201 tctttaagta aagtatcatc agggttcaca cctaattcta atagataaag tacactatca
     7261 agagtagaga aaggagaacc ttttattaaa cgcttatcag gtgtagtcca atagtctgca
     7321 ccattatcat acctgtgtga taatattatt tcaatatctg ataaagattg attttctaca
     7381 ttcatttttt actccatttc atattatatt ctattaaatc tatcaaaaac tttattataa
     7441 tattatttat atttataatt gatttatttt atttaaaatt gcatttatag ctgtatcagg
     7501 aacaactgct aaatcttctg cattgaagta ataaactata ttagaatcaa ataatatatt
     7561 tgaatttgga ggaaattcat catctccaaa ccaaagtata aataccatag atgtattacc
     7621 catatatgta aacctataag ctaaatctcc catgtttact ttttcagctc caattttctc
     7681 cataactaac ttatatttgt ctatttgaga tgtaaaaact tctgaaagcc tatatagagt
     7741 cctgtttaaa aaatttggat aataaacatt gcctcctggt atttctttaa aagttacata
     7801 tttattagtt ctaggaatac ctttagcatt gattaaatat ctaactatca agattttaag
     7861 ctcaaagtta ttgcaaaggc taccattact attccaaact tctccacttg gatagtctat
     7921 aatatattgt ctatcaaaaa aattgagtgt gaatctattt aaatcattat tgaaaggtat
     7981 attacttaaa ttactaattg tatgagggtc taattctttg agtttttttc gaacatagtc
     8041 aaatggaaca ttataattat tttttgttat cttttcatcc ataatttttt cctccaattt
     8101 aaacaattaa aagtatattt ataaaaagtt ttc
//
LOCUS       DAMJYR010000154         8126 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.159, whole genome shotgun sequence.
ACCESSION   DAMJYR010000154 DAMJYR010000000
VERSION     DAMJYR010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8126)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8126)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8126
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.159"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(<1..766)
                     /locus_tag="PC471_003308"
     CDS             complement(<1..766)
                     /locus_tag="PC471_003308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HDF2790412.1"
                     /translation="MLNIYISEIDLIKAQLKKESTFNLVKVIEHYILKHRPEKYKQGE
                     EYYYGNTDVNNKRRYYLLDGAKVDDFTKVNNKAVNNYHKLLVDQKVGYSVGNPIVFNA
                     DDNDFTKLLNDLLGEEFDDTITELYLNASNKGIEWLHPYINRKGEFKYVIIPAEEAIP
                     IWDSKRQRELVAFIRFYYIEDIDGNKIKRVEYYTENDVTYFIERGNSFIQEFLYDEYG
                     KMTDIQEGHFRINNKEQGWGKVPFIPFKNNEKCVSDL"
     gene            complement(772..2025)
                     /locus_tag="PC471_003309"
     CDS             complement(772..2025)
                     /locus_tag="PC471_003309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HDF2790413.1"
                     /translation="MVIAMRVRIDFNPSFKEANFTKKRYRAMKGSAGSGKSVNVAQDY
                     ILKLGDKKYQGANLLVVRKSEATHKYSTYAELTGAINRIYGKQADKYWKTTLNPLEIK
                     SKITGNSIIFRGVNDAKQREKLKSINFSKGKLTWVWCEEATELMESDIDILDDRLRGI
                     LTNPNLYYQMTFTFNPVSATHWIKRKYFDYKNDDIFTHHSTYLQNRFIDEAYYRRMQM
                     RKEQDPEGYKVYGLGEWGETGGAILKNYVIHEFPTEFEYFDNMRLSQDFGFNHANVVL
                     RIGFKDGELYICNEIYVHEMDTSEIIKIANSKGLEKNLFMYCDSAEPDRIKMWKSAGY
                     KAKGVKKGPGSVKAQIDYLKQLRIHVHPSCANTIKEIQQWKWKQDERTGLYLDEPVEF
                     MDDAMAALRYSIDNKLKNNGISFLK"
     gene            complement(2003..2686)
                     /locus_tag="PC471_003310"
     CDS             complement(2003..2686)
                     /locus_tag="PC471_003310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2790414.1"
                     /translation="MQDVKEKVKQDYIKGMKQKEISAKYDISLNTLKSWIKRYNWASE
                     KKKGAPKNKRGAPIGNKNATGPPGNKNAEKFGFFSKYLPEETQDLINEIKNKDKFDIL
                     WEQITIQYAAIIRAQKIMYVKDKEEMIKELKKHESTENGEKIEYEFQFAWDRQASFLN
                     AQSRAMSELRSLIKQYDEMIHKDWNLATEEQKNRVEKLKCEVDNLSKDDIGDDELKIS
                     VDYGDSNES"
     gene            complement(2749..3427)
                     /locus_tag="PC471_003311"
                     /pseudo
     CDS             complement(2749..3427)
                     /locus_tag="PC471_003311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015994390.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Bro-N domain-containing protein"
     gene            complement(3680..3886)
                     /locus_tag="PC471_003312"
     CDS             complement(3680..3886)
                     /locus_tag="PC471_003312"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2790415.1"
                     /translation="MSNLREKIIKELNEFNIDADDEFLSYGVEYVESFIGKSAISEEL
                     LIRGVVFTANYIVNMKAKDGVNKA"
     gene            complement(4326..4808)
                     /locus_tag="PC471_003313"
     CDS             complement(4326..4808)
                     /locus_tag="PC471_003313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015994385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2790416.1"
                     /translation="MNKKTLFQEVEGRLYNYKKLESQIRIKDIYIKKLESEFCGCKAQ
                     SYEEKTGETYNISSSVENEVIKREEDLNRLKEDKKTLEIEKETIECALTSLNSFETEF
                     FNEMYMNNEKINMDYMSNAMHIDRSHCFRIRKRIVCKIMDMLYPKIKEFELPIFSWKA
                     "
     gene            complement(4912..5649)
                     /locus_tag="PC471_003314"
     CDS             complement(4912..5649)
                     /locus_tag="PC471_003314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antA/AntB antirepressor family protein"
                     /protein_id="HDF2790417.1"
                     /translation="MNNLKLIENEGLIKVYTTDEDIKVVNGRELWEGLGVKQQFSDWI
                     ETNLKNVDANKNEFYILKGKTSKQGGRPTDEYIIKLEIAKEICLVAGASPRANKELKR
                     NSKNYRKYLIAVEEKYKVSNNLTKTQLNQINDIVSNALCEMQTKHDAQIEQFKKESSQ
                     YYRPTSKTKYDISSYIKNRLDIPRANEEFDLVKKRTLLILGADKWEDIPKDVLLKSLN
                     IIDESIRIIKSERKTNQISFFEKDNFC"
     gene            complement(5712..6119)
                     /locus_tag="PC471_003315"
     CDS             complement(5712..6119)
                     /locus_tag="PC471_003315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HDF2790418.1"
                     /translation="MKVNFVIDGEPVGKERPRMNSITKRTYTPNKTKNYEELIKWLYQ
                     SKVKYRFTGYIKMTLRCYYSIAKSNSKKVKEQKRNNVLRPSKKPDIDNVVKIIADSLN
                     EIAYKDDTQIVEVVASKYYSDNPRVEVILEDIS"
     gene            complement(6109..6507)
                     /locus_tag="PC471_003316"
     CDS             complement(6109..6507)
                     /locus_tag="PC471_003316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2790419.1"
                     /translation="MQKDIWLYSWDDEYFASDEYESKEEAINGAKEELKRFKEVKQCV
                     YIGKKEDVNIPNIDAEDALERVQDRIDDEFGGFGEDWFENIRAEDILTLENRISKVFK
                     KWIDEFGYKPYWFVVRDTEEIELNEVANES"
     gene            complement(6646..6843)
                     /locus_tag="PC471_003317"
     CDS             complement(6646..6843)
                     /locus_tag="PC471_003317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2790420.1"
                     /translation="MKNKVEINNIQQLNDALNKYNIPFDVLSDIDRRICGWMATGGNE
                     DDAYIKQQYRYVENFINLFCD"
     gene            complement(6939..7268)
                     /locus_tag="PC471_003318"
     CDS             complement(6939..7268)
                     /locus_tag="PC471_003318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015994378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2790421.1"
                     /translation="MKIFLLTILLIIICILANYVKNRIYKKSINNLKYKYSVGEKIIY
                     HQINCYYNRMVGCEILEKCYSTKFRKRNTPLYKVKAYVGDNNTTWVIPEWRIECLATT
                     YGEFPKY"
     gene            complement(7350..>8126)
                     /locus_tag="PC471_003319"
     CDS             complement(7350..>8126)
                     /locus_tag="PC471_003319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HDF2790422.1"
                     /translation="KNSKEIASYLFDVMKINAALESCDLAICLYTIKDEKSVAILKLD
                     YKKLYTHSIEFVDDKFNIQMVSNEIGIPETLRQKQGALISLSGINDEFHLRLLDKDAE
                     KEGSESKFVTEFLNAKKIDDDKYKTKVFKNTAENWITNALSNDIKQAEDVRSILNYTL
                     KEKHEVDINDFVDNSIKDDELKDSFKEHMEEKGLDESFSIDKKWVEKKLKKRSIKTDN
                     GFDIKGNLTDFEDPMKYTVRQNQNGSIDIIIKNVTFYEEK"
BASE COUNT         2363 a         1562 c          790 g         3411 t
ORIGIN      
        1 ttaaatctga gacacatttt tcattatttt taaaaggtat aaatggaact ttaccccagc
       61 cctgttcttt gttatttatt ctaaaatgac cttcttgtat atcagtcatt tttccatatt
      121 catcatataa aaattcttga ataaaactat tacctctttc aataaagtaa gttacgtcat
      181 tttctgtata atattcaact ctttttatct tgtttccatc tatatcctca atataataaa
      241 acctaataaa tgcaactaat tccctctgtc ttttactatc ccaaatagga attgcttctt
      301 cagctggaat tattacatat ttaaactcac cttttctatt aatatatgga tgtaaccatt
      361 caatcccttt attactagca ttgagataga gttctgttat tgtatcgtca aattcttctc
      421 ctagtaaatc atttaaaagc ttagtaaaat cattatcatc tgcattaaat acgattggat
      481 ttccaacgct atagcccact ttttggtcaa ctaaaagctt atgataatta ttaactgctt
      541 tattattaac cttagtaaaa tcatcaacct tagctccatc taagagataa taccttctct
      601 tattgtttac atcagtatta ccataatagt attcttctcc ttgtttgtat ttctctggtc
      661 tatgctttaa gatgtagtgt tctatgactt ttactaggtt aaaggtactc tcttttttta
      721 actgagcttt tattaaatct atttcactta tataaatatt taacacctcc tttactttaa
      781 gaagcttatt ccattatttt taagcttatt atctatagaa tatctaagag cagccattgc
      841 atcatccata aactcaactg gttcatcaag atataatcca gttctttcat cttgtttcca
      901 tttccattgt tgtatttctt ttatggtatt agcgcaacta ggatgtacat gtattcttaa
      961 ttgtttcaaa taatctattt gagctttaac actccctggt ccttttttaa ctcctttagc
     1021 tttatatcct gcactcttcc acatcttaat tctatctggt tcagcactat cacagtacat
     1081 aaatagattc ttttctaaac ctttactatt tgcaatcttt atgatttctg aggtatccat
     1141 ttcatgtaca tatatttcat tacatatata taactctcca tccttaaagc caattctaag
     1201 tactacattt gcatggttaa atccaaagtc ttgtgataac ctcatattat caaaatactc
     1261 aaattctgta ggaaactcat gtataacata gtttttaagt attgctccac cagtttctcc
     1321 ccattctcca agtccataga ctttatatcc ttctgggtct tgctcttttc tcatttgcat
     1381 tcttctatag taagcttcat ctataaatct attttgtaga taagtactat gatgagtaaa
     1441 tatatcatca tttttatagt caaaatactt tctttttatc caatgggtag ctgagactgg
     1501 attaaatgta aacgtcattt gataatacaa gttaggatta gttaaaatac ctcttaaacg
     1561 gtcgtctagt atgtctatat cactttccat aagttctgta gcttcttcac accaaaccca
     1621 tgttaatttt ccttttgaga agttaattga ttttaatttt tctctttgct tagcatcatt
     1681 aacccctctg aaaattatag aattaccagt aattttactt ttgatttcta aaggatttaa
     1741 agttgttttc caatacttat cagcttgttt accataaata cgatttatag ctcctgtaag
     1801 ttctgcatat gttgagtatt tgtgcgtagc ttctgatttt ctaactacta atagattagc
     1861 tccttgatac tttttatctc ctaactttag tatataatcc tgtgctacat taacagattt
     1921 tccactccct gctgaacctt tcattgctct gtatcttttt ttagtaaaat tagcttcttt
     1981 aaaagatgga ttaaaatcta ttctaactct cattgctatc accataatct acacttattt
     2041 tcaactcatc atccccaata tcatctttac ttaggttatc aacctcacat tttaacttct
     2101 caactctatt tttctgctcc tctgtagcca aattccaatc cttatgaatc atttcatcat
     2161 actgcttaat aagactcctt aactcactca ttgccctact ctgtgcatta agaaaagatg
     2221 cttgcctatc ccatgcaaat tgaaattcat actctatctt ctcaccattt tctgtgcttt
     2281 catgtttctt taattcctta atcatttctt ccttgtcttt aacatacatt atcttctgtg
     2341 ctcttattat tgctgcatat tgtattgtta tctgttccca aagaatatca aatttatctt
     2401 tattctttat ctcattaatt aagtcttgag tttcttcagg taagtatttt gagaagaaac
     2461 caaacttttc agcattttta ttaccaggtg gaccagtagc gtttttatta cctatgggtg
     2521 cacccctttt atttttaggt gcacccttct ttttctcact agcccaattg tatcttttta
     2581 tccatgactt taaagtgttt aaactaatgt catactttgc tgatatttcc ttttgtttca
     2641 taccttttat gtaatcttgt tttacctttt ctttgacatc ttgcacatca ccacctcgtt
     2701 tgtttgtcgt tttgggaatt aaaaaagacc ctccatcaag acagtcattt aaatcatttc
     2761 tattaattct ttaatctttt tatatacttc tttataattc atatctttat ctattaactt
     2821 aggtaatttc atagatataa ttctttcaag tgcttgtata tcaaacagtt cactttgatt
     2881 taactcatcc cttttcacac cttttggtat acctaatttt tttcttacaa gttcggtaaa
     2941 atgtttataa tacatctgag gtttattact tccttgacta attgcataat aaacaaattc
     3001 ttgtatttca tctgtaaaat cttttcttac tttttttacc ttctgttctt atatccaacc
     3061 attcttggtc tttttctgta gcaatgtagt aaccatgtat tctaatttgt ttaagtgttt
     3121 gtgtaaccca ttttgtaaat aactttgctt ctggtttatt acttctaaat gacatattgt
     3181 acacagcttc ttctgtaaca aaagtagtac cgaagttagg taatttatct ttaaagtttc
     3241 tagtgtagga atctccgaca gtagactcat taaatttctt tttatattct ctatctatat
     3301 ttcttaatgt atcacgaata tttactatgc ctagttcctc tcctacgtca tttgcattaa
     3361 accaaacttc ttctccattt ttggaccaca ttacttttac atttttctct tgtaaaattt
     3421 tcaacatact actacctcct aaattattta tattaataaa gggtgctcaa tttgaacatc
     3481 ctttttatat atcgacacct caatttgacc ccccgtcatt tcataaaatc aacctcctgg
     3541 gcatttgaat tttagatatg catagtgtgc atatccgaat agtgcatatt atgcattttt
     3601 aatagacctt ttcagatgtc ggagttaccg acatctgaaa cttattaatt tttgtattaa
     3661 aaaagaccta gaaattaatt taagccttat ttacaccatc ttttgctttc atattcacta
     3721 tataatttgc tgtaaacact actcctctta tcaataactc ttcactaata gcacttttcc
     3781 ctataaaact ctcaacatat tcaactccat aacttaaaaa ttcatcatct gcatctatat
     3841 taaattcatt tagttctttt attatctttt ctcttaaatt actcatttat catactcctg
     3901 taaaaaaata aaagactaag cttaccctca cttagccttt ttaaataggg agatacatat
     3961 attatgtcgc aagttctaag aatcgaactt agattaagca ccagcacctg catggtgagt
     4021 gaggttacca agccccaccc gatttttaga cttctgaatt aagatacaaa atataaaatt
     4081 ttgccctcaa tttctctact tttagcgtat acgttgatta atatttgaac atagttagaa
     4141 ttgaactaac aacgtcctca cgccctgcct agtctgttca tattgctaga ttagcccttt
     4201 aaactaacct agcaattatt tagttttgag agggaaatct ttatttccac gatattatta
     4261 tctcatgttt ttgccaacaa aaagtctcac gatagtctca aaaaagtctc aaaatagtct
     4321 catttttaag ctttccatga aaaaatgggt aattcaaact cttttatttt tggatataac
     4381 atatccataa ttttacaaac tatccttttt cttattctaa agcaatgact tctatctatg
     4441 tgcatagcat tagacatata atccatgttg attttctcat tgttcatata catttcattg
     4501 aagaactctg tttcaaagct atttagactt gttaatgcac attctatagt ttctttttca
     4561 atttctaatg ttttcttatc ttcttttaat ctatttaaat cttcttctct cttaataact
     4621 tcattttcaa cacttgaact tatattataa gtttctccag ttttttcttc ataactttga
     4681 gccttacatc cgcaaaactc actttctaat tttttaatat atatatcttt tattcttatt
     4741 tgactttcta gttttttata gttatacaat ctaccttcaa cctcttgaaa tagtgtcttt
     4801 ttattcatac ttccacactc ctatcaatta tttatgttat aataatattt gtatataaaa
     4861 gttttatatt tttgacaact ggagtgtgaa agcactcctt ttttcttttt attaacagaa
     4921 attatctttt tcaaagaaac taatctgatt tgtctttctt tcagacttta taattctaat
     4981 tgattcatct ataatgttta gggattttaa taacacatct tttggtatgt cttcccactt
     5041 atcagctcca agaattaata aagttctctt tttaactaaa tcaaactcct cattagctct
     5101 aggaatatct aatctatttt ttatgtagct tgaaatatca tacttagttt tacttgtagg
     5161 tctataatat tgtgaggatt cctttttaaa ttgttctatt tgtgcatcat gtttggtttg
     5221 catttcacat aatgcgttac ttacaatatc attaatttga tttaactgtg tttttgtaag
     5281 attgtttgat actttgtatt tttcttctac tgcaattaga tattttctat aatttttaga
     5341 gtttctttta agttctttat ttgctcttgg acttgctcct gctactaaac aaatttcttt
     5401 tgctatttct aatttaatta tatattcgtc tgtaggtctt ccaccctgtt tagaggtttt
     5461 accttttaaa atgtaaaact catttttatt agcatccaca ttttttaagt ttgtttctat
     5521 ccagtctgaa aattgttgtt ttactcctaa tccttcccaa agttctcttc catttacaac
     5581 ttttatatct tcatctgttg tatatacttt tataagtcct tcattctcga ttaattttaa
     5641 attattcata cttataatcc tcctccaaat tatttttatc taatgatttg ataaatggat
     5701 ttttccattg ttcaactaat atcttctaat ataacctcaa ccctgggatt gtcactataa
     5761 tatttactag ctacaacctc aacaatctgt gtatcatcct tataagctat ctcattgagt
     5821 gaatcagcta taatcttaac aacattgtct atatcgggtt tcttactagg tcttaataca
     5881 ttatttcttt tctgctcttt aaccttttta ctgttacttt tagctataga atagtaacat
     5941 cttaaagtca tttttatata accagtaaaa cgatacttaa ctttagattg atacagccat
     6001 tttattaact cttcataatt cttagtctta ttaggtgtat aggtcctttt agttatagag
     6061 ttcattctcg gtctttcttt gccaactggc tctccatcta ttacaaaatt aactttcatt
     6121 tgctacctca tttagttcta tttcttccgt atctctaaca acaaaccaat atggtttata
     6181 tccaaattca tctatccatt ttttaaatac tttgcttatt ctattctcta gtgttaatat
     6241 gtcttcagca cgtatatttt caaaccaatc ttctccaaac cctccaaatt catcatcaat
     6301 tctatcttga acacgttcta aagcatcctc tgcatctata ttaggtatat taacatcttc
     6361 tttttttcca atataaacac attgtttaac ctctttaaac cttttaagtt cttccttagc
     6421 tccattaata gcttcctctt tgctttcata ttcatcactt gcaaaatatt catcatccca
     6481 actatataac caaatatctt tttgcatatt aatacctcca ctatttattt ttcttattag
     6541 ccttctttct acagtcctta caacaataaa tctccttaga tttttcctta agataaaaca
     6601 gcttaccaca ccaactgcat cttcttcgtt tcatagaatc acttcctagt cacaaaacaa
     6661 atttataaaa ttctctacat atctatattg ttgtttaata taagcatcat cttcattacc
     6721 accagtagcc atccagccac atattcttct atctatatca cttaacacgt caaatgggat
     6781 attatactta tttaaagcat catttaattg ttgtatatta tttatctcaa ccttattttt
     6841 catatgcaaa tcaccctttt tataagtcaa agtaagtcta taaacttcta gtttcattca
     6901 caaacttacc ttgactattt atactccatt tttattattt aatatttagg aaattctcca
     6961 taagtcgtag caagacattc aattctccac tctggtataa cccatgttgt attgttatca
     7021 cctacatatg cttttacttt ataaagcggg gtatttcttt ttctaaattt cgtactataa
     7081 catttttcta aaatttcaca accaaccatt ctgttatagt aacagtttat ttgatgatat
     7141 ataatctttt cccctacaga atatttatat tttaaattat ttatagattt tttatatatg
     7201 cgatttttta cataatttgc taatatacaa ataattatta gcagtatagt caataaaaaa
     7261 atcttcataa gttattcctc ctaactagtt taaatttaca tcctcttatt atttacttct
     7321 ccttctctag ccaatagtca catgagtacc tacttttcct cataaaatgt tacattctta
     7381 ataattatat ctatagaccc attttgattt tgtcttacag tatacttcat tgggtcttca
     7441 aaatcagtta gattcccttt tatgtcaaaa ccattatccg tttttatact tcttttttta
     7501 agctttttct caacccattt tttatctata ctaaaacttt cgtcaagacc tttttcttcc
     7561 atatgctctt taaagctatc ctttaactca tcatctttaa ttgaattatc aacaaagtca
     7621 tttatatcaa cttcatgttt ttccttcagt gtataattta atatacttct tacatcttca
     7681 gcttgcttta tatcattact aagagcattg gttatccaat tttcagctgt atttttaaat
     7741 accttggtct tgtacttatc atcatctatt tttttagcat ttaaaaattc tgttacaaac
     7801 ttagattctg acccttcttt ttcagcatcc ttatctaaaa gccttagatg aaactcatca
     7861 tttataccac ttagactaat caaagctcct tgcttttgtc ttaaagtctc tgggatacca
     7921 atttcatttg aaaccatttg aatattaaat ttatcatcca caaattcaat tgaatgagtg
     7981 tacaactttt tataatctaa tttcagtatt gctacactct tttcatcttt aatagtgtat
     8041 aaacaaattg ccaaatcaca agattctagt gcagcattaa ttttcataac gtcaaacaag
     8101 taagatgcaa tttctttaga attttt
//
LOCUS       DAMJYR010000155         7957 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.160, whole genome shotgun sequence.
ACCESSION   DAMJYR010000155 DAMJYR010000000
VERSION     DAMJYR010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7957)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7957)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7957
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.160"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(269..1423)
                     /locus_tag="PC471_003320"
     CDS             complement(269..1423)
                     /locus_tag="PC471_003320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDF2790423.1"
                     /translation="MINKAIKFKFIIFYFFSYIIFAFASTKFTPFLSKLGYSAFERGI
                     ILSSYAITNILFQLLFGVLADKYQTMKKIVLISLSVYGIASVIMFSTESATFIIYLLL
                     VALSGGLLNTCCSLYDTWIIGCGDNINNYLSFIKTFGSIGWAIGSMVASYLILRFSYF
                     GLSVAIGLIVVLLIINILLLPDIEKINKKINVTNKDIIELLKDKKYILLVCILFLLYS
                     MVVANNCTVIDKMIALNATNSQISLKWSLQSLLEIPTYLAGSYLLKKYSSLNLLKLSA
                     IMITVQFLLFALTNDSNIIIILCVFQVFSTPLILITSKRLIFEVSPKKLRSSSQLIAL
                     SIFMGGSSLIIPTVAGFLSINIGYNYTLMVLSLFGCLAYCLIVLLNYLQE"
     gene            complement(1426..1893)
                     /locus_tag="PC471_003321"
     CDS             complement(1426..1893)
                     /locus_tag="PC471_003321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDF2790424.1"
                     /translation="MFFKRKKEAITINSYIDGKIINLGEIPDKVFSTKVMGDGFAMIA
                     KDNILKSPTKGVISFIAQTNHAIGIKLQNDVQILIHIGLDSATTKKENFKLEVKQGQE
                     VNMGEKLVEIDNKLLENTLYIPLVIVENPNTVKYHLSNLNQEGNSGIKVINFD"
     gene            complement(1907..2395)
                     /locus_tag="PC471_003322"
     CDS             complement(1907..2395)
                     /locus_tag="PC471_003322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2790425.1"
                     /translation="MITNKIKNITAVLKDENIFLSVVMVCFLILPVLYIVYLELTSLF
                     TNISFSQLLNNDVNASLNLISNFSAVYSAYVIYNIKKNKEIKENMIAFYLVLLGQIFQ
                     IYTITIVIMFTYIYKFVGIKQIISSIKELQWGKSYKALFSSIAVCLISLFVMIIRIKL
                     IM"
     gene            complement(2409..3740)
                     /locus_tag="PC471_003323"
     CDS             complement(2409..3740)
                     /locus_tag="PC471_003323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-alpha-glucosidase"
                     /protein_id="HDF2790426.1"
                     /translation="MERKRQRILIAGGGSTYTAGIVTMLIESVAKFPIESIKLYDNND
                     ERQRKVAEACAIIVREKNPEIKFSYTTNPEEAFTDIDFVMAQIRVGLYALREQDEKIP
                     LKYGVVGQETCGAGGIAYGLRTIGPIIEMIDYMEKYSPNAWMLNYSNPAAIVAEACRV
                     LRPNSRIINICDMPVCLEEIFARVLGLNSRKDFDVRYYGLNHFGWWTSIKDKEGNDLM
                     PKLQEYCAKKGYEEFTPQGQHKESSWLETMRAAKDLLEIEPTTLPNTYLKYYLMADET
                     VEHANPNYTRANEIMDRREKDTFEECERIIKNGTARDTWFDASEHSQFIVELACALAF
                     NTQERFLLIVPNNGAIENFADDAMVEIPCLVGKDMVEPMSIGKIPTFQKGLMEQQVAS
                     EKLAVEAWIEKSYQKLWQSFTMSKTVPSAKVAKEILDEMIVANKDFWPTFK"
     gene            complement(3754..5319)
                     /locus_tag="PC471_003324"
     CDS             complement(3754..5319)
                     /locus_tag="PC471_003324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003233606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDF2790427.1"
                     /translation="MMKKIQRFGGAMFTPTLLFAFAGIMVGFSIVFQNQSIMGSLATP
                     ENIWYQFWGVISSGAWMVFNQLPLLFAISLPIALAKKQQARACMEALATYLTFNYFVG
                     SMLSFWGKSFGVDFAAEISAGSGLVSIAGIKTLDTGMVGALLISGIVIYIHNRFYDKE
                     LPDFIGLFRGSSLVVAICFFVMIPVALLTCFIWPHIQNVIRHLQTFFINSGNIGVWCY
                     AFLQKILIPTGLHHFVYAPICYDSVVVPGGTSVYWATHIQDFQTSAKTLKEMYPIGFS
                     LSGLSKVFGSLGVFGAFYVTAKPEKKKKVLGLMIPATLTAVLTGITEPLEFTFLFVAP
                     LLFLVHAFLDACLQTISFALGVVGDFGGGIINWVVLNWLPLGMYHWKVYVVQVVVGII
                     FSFIWFFVFTFLIKKFDMKTPGREEDSEETKLYTKNEYLETKDEKGNKLSKASQQASE
                     YIKLVGGAENVVDVTNCATRLRLTLKDDSIISKEEDFKAVGAHGLVHNGKAIQIIIGL
                     SVPSVREEFENLL"
     gene            complement(5489..6274)
                     /locus_tag="PC471_003325"
     CDS             complement(5489..6274)
                     /locus_tag="PC471_003325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDF2790428.1"
                     /translation="MRLEEMIFLNYNKLNDTDLLIWKYITANKKHCSKMSINELAMKC
                     NVSRATITRFVKKLDLKGFSEFKVLLSWESEKVHVIENQSFDIACNSIIKYVEDQKNK
                     DYDALCKLLYESRTIYAYGSGDIQNVVAKQIKRMFLSCQEIIYDFEGRTFDDSFYNIV
                     SKNDVMIIISLSGENENTLNIARRLKLIGTKIISITEFKNNTLTDLSDESIYISATNI
                     SFLQMHPSYKLTMLYFILIELLFIKYSIYKRHRMITEGIDCKL"
     gene            complement(6441..7640)
                     /locus_tag="PC471_003326"
     CDS             complement(6441..7640)
                     /locus_tag="PC471_003326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase S41"
                     /protein_id="HDF2790429.1"
                     /translation="MKKKVLFLVIILAFIILLGNSTYSQKNQLSEEEKVDDFNYVYNV
                     VKTGYPYLGVNKRLNNIDWLANKDEYTKRIKNTENDEEFIEELSSILSDLNNKHTEVI
                     DNKKRYELFKKSYSNNDWYDFLNDKKVVDRYDSMNLKIKMPDDIFVKKELILKDVIKG
                     QIGYMYLPSMSSKNGSINNDLKIIGDYINTLEKHKALIIDIRGNLGGSDRYWQGIVSK
                     LIKNDVKINGYRIYRNNNEIVKKYTNARNIKLNPVENLPASVKENAPKEIIKDFNDFE
                     DTSYTIKSTDDSKFKGNIYLLVDRKIYSSSESFAMFCKENKFATLVGETTGGDGGCID
                     PVLFNLKNSGLIVRMASCMYLNKNGICDEEFKTIPEFKVKDCKRTADFKDDNCIRKAL
                     ELENINY"
BASE COUNT         2825 a         1350 c          810 g         2972 t
ORIGIN      
        1 ggttttatat aaataaatcc ttaagtaaat attacatttt aactgttagc attacacttt
       61 tgtaacatat ggaagactat tataattata gtagaaatat cttaaaataa aagcatgatt
      121 tacttctttg tataaacaaa gaaaagaagc tcctaagaag cctctaatct atacaattat
      181 tcttctctat aaaaaaccaa aatacacatt gattgtggca aatacagata ctataaacaa
      241 gtgatattac tactatatat taaaacaatt attcctgaag ataattcaat aagactatca
      301 agcaataagc taagcatcca aaaagactta ataccattaa tgtataatta tacccaatat
      361 taatgctcaa aaaaccagca acagttggaa taatcagaga tgaacctccc ataaatatac
      421 ttaaagctat taattggcta ctacttctaa gttttttagg agaaacttca aatattaatc
      481 tttttgaagt aattaaaatt aaaggagtag aaaacacttg aaaaacacac agtataataa
      541 tgatatttga atcatttgtt aaagcaaata atagaaactg aactgttatc ataatagcac
      601 ttaattttaa taagtttaaa ctgctatatt tttttaataa ataagaacca gccagatatg
      661 ttggtatctc taacaatgac tgtaaagacc actttaaaga tatctgagaa tttgtagcat
      721 ttaaagctat cattttatct attacagtac agttatttgc taccaccatt gagtataata
      781 agaataaaat acatactagt aagatatatt ttttatcttt taataattca ataatatctt
      841 tattagttac atttattttc ttattaatct tttcaatatc aggtaataat agtatattta
      901 taataagtaa tactacaatt aaaccaatcg ctacagataa tccaaaatat gagaatctaa
      961 gaatcaagta agatgctacc atacttccaa tagcccatcc aatcgaccca aaagtcttaa
     1021 taaatgacaa ataattatta atattatctc cacaacctat tatccatgta tcatataaac
     1081 tacagcaagt atttaataat cctcctgata aagcaacaag taataagtat attataaaag
     1141 ttgcagattc agtagaaaac ataataactg atgcaattcc ataaactgat aaactaatta
     1201 aaacaatttt tttcattgtt tgatacttat cagctaatac tccaaataat aattgaaaca
     1261 atatattggt tattgcatag ctcgataata taattcctct ttcaaacgct gagtaaccta
     1321 attttgataa gaaaggtgtg aattttgttg aagcaaaggc aaaaataata tatgagaaaa
     1381 aatagaatat gataaattta aatttaatcg ctttattaat cataatcaat caaaattgat
     1441 aactttaata ccactatttc cttcctgatt taagtttgaa agatgatatt ttaccgtatt
     1501 aggattctct acaataacta aaggaatata taaagtattt tcaagtaact tattatcaat
     1561 ttctactaac ttttccccca tattaacttc ttgaccttgt ttaacttcta atttaaagtt
     1621 ttcttttttt gttgttgcac tatccaatcc aatatgaata agaatttgaa catcattttg
     1681 caatttaatt cctatagcat ggttagtttg agcaataaaa cttattactc ctttagtcgg
     1741 tgatttaaga atattgtcct ttgctatcat agcaaatcca tctcccatta ccttagttga
     1801 aaatacttta tcaggaattt ctccaagatt aattattttt ccatcaatgt atgaattaat
     1861 tgtaattgct tctttttttc ttttaaagaa cattatagac actcctctac ataattaact
     1921 ttattctaat tatcattaca aataacgata ttaagcatac tgcaattgag gaaaaaagag
     1981 ccttatagct ctttccccat tgtagttctt taatactgct tatgatttgt ttaattccaa
     2041 caaatttata aatataggta aacatgataa caatagttat tgtgtaaatt tggaatatct
     2101 gccctaataa gactaaatag aaagcaatca tgttttcttt tatctcttta ttctttttta
     2161 tattgtaaat aacatatgca ctgtatactg cactaaagtt agagattaaa tttaaacttg
     2221 cattaacatc attgtttaaa agttgtgaaa atgaaatatt tgtaaataat gatgtcaatt
     2281 ctaaatacac tatatataaa acaggtaata ttagaaaaca aaccataacc actgaaagaa
     2341 atatattttc atcttttaat actgctgtga tattttttat tttatttgtt atcatgtttt
     2401 tgttctcctt atttaaaagt tggccagaaa tccttattag ccacaatcat ttcatctaag
     2461 atttcttttg ctacttttgc acttggaaca gttttactca tagtaaacga ctgccataat
     2521 ttttgataac ttttttctat ccatgcttct acagctaatt tttcagaagc aacttgttgt
     2581 tccattaaac ctttttggaa agttggaatt ttacctatag acataggttc aaccatatct
     2641 tttccaacaa ggcaaggaat ttcaaccatt gcatcatctg caaaattttc aatagctcca
     2701 ttattaggaa caatcagtaa aaatctttcc tgagtattaa atgctaatgc acaagctaat
     2761 tcaacaataa attgagaatg ttcacttgca tcaaaccatg tatctcttgc tgtgccattt
     2821 tttataattc tttcacattc ttcgaatgta tccttttctc ttctatccat aatttcattt
     2881 gctcttgtat aatttggatt agcatgttca acagtttcat cagccattaa ataatatttt
     2941 aaataagtat taggtaatgt agtcggttct atttccaata aatcctttgc tgctctcata
     3001 gtttctaacc aactagattc tttgtgttga ccttgaggtg taaattcctc atatcctttt
     3061 ttagcacaat attcctgtaa tttaggcatt aaatcatttc cttctttgtc cttaatactt
     3121 gtccaccacc cgaaatgatt caatccataa tatctaacat caaagtcttt acgagaattt
     3181 aaacctaaaa ctctagcaaa tatttcctct aaacatactg gcatatcaca aatattaata
     3241 attcttgaat ttggtcttaa tacacgacat gcttctgcaa ctatagctgc tggatttgaa
     3301 taattaagca tccaagcatt tggagaatat ttttccatat aatcaatcat ttcgattatt
     3361 ggacctattg ttcttaatcc ataggcaatt ccacctgccc cacaagtttc ttgaccaaca
     3421 accccatatt ttagtggaat tttttcatct tgttcacgta aagcatataa accaacacga
     3481 atttgtgcca ttacaaaatc aatatcagta aatgcttctt ctggatttgt tgtataacta
     3541 aatttaattt ctggattttt ctctctaaca attattgcac atgcttcagc aacttttctt
     3601 tgtctctcat cattattatc atataattta attgattcaa taggaaattt agcaacactt
     3661 tcaattaaca ttgttacaat tccagcagta taagtactac ccccaccagc aattagtatc
     3721 ctctgtcttt ttctttccat ctttccttct cctttataat aaattttcaa attcttctct
     3781 tacacttggt actgaaagcc caataataat ctgaatagct tttccattat gcactaaacc
     3841 atgtgcacct actgccttaa aatcttcttc tttagaaata attgagtcat ctttcaaggt
     3901 taatctcaac ctcgtagcac agtttgtaac atctacaaca ttttctgctc caccaactaa
     3961 ttttatgtat tcagatgctt gttgagaggc tttactcaat ttatttcctt tttcatcttt
     4021 agtttctaag tattcatttt tagtatatag ttttgtttct tcactatctt cctctctacc
     4081 aggagttttc atatcaaact tcttaattaa gaaagtaaat acaaagaacc agataaatga
     4141 aaatataatc cctacaacaa cttgaacaac atataccttc cagtgataca ttccaagagg
     4201 aagccaattt aatacaaccc agttaataat tcctccacca aaatcaccaa caactcctag
     4261 ggcaaacgaa atcgtttgca agcatgcatc taaaaatgca tgtactagga ataaaagtgg
     4321 tgctacaaat aaaaatgtaa attctaacgg ctcagtaatt ccagttaata ctgcagtaag
     4381 agtagcagga atcattaaac ctaatacttt tttctttttt tctggctttg cagttacata
     4441 gaaagcacca aatacaccta atgaaccaaa caccttcgat aatccggata atgaaaatcc
     4501 gattggatac atttccttta gagtcttagc tgaagtttga aaatcctgaa tatgagtagc
     4561 ccaatatact gatgtaccac ctggaacaac aacactatca taacaaattg gtgcatatac
     4621 aaaatgatgt aatccagttg gtattaaaat cttttgtaag aatgcataac accaaactcc
     4681 tatatttcca ctatttataa aaaacgtttg caaatgtcta ataacatttt gaatatgagg
     4741 ccaaataaaa caagttaata atgcaactgg tatcataaca aagaaacaaa ttgcaactac
     4801 aagtgatgag cctctaaata atccaataaa atctggcagt tctttatcat aaaatctatt
     4861 atgaatataa atgacaatac ctgatattaa caaagcacca accataccag tatctaatgt
     4921 tttaatacca gcaatgctta caagtccact tccagcactt atttcagctg cgaaatcaac
     4981 tccaaagctt tttccccaaa aacttaacat agaacctaca aaataattaa aagtcaaata
     5041 cgttgctaaa gcctccatac aagctcttgc ttgttgcttt tttgcaagtg ctattggtaa
     5101 agaaatagca aataataatg gcaattgatt aaataccatc caagctccac tagatattac
     5161 accccaaaat tggtaccaaa tattttctgg tgtagcaagt gaacccataa ttgactgatt
     5221 ttgaaataca attgagaatc caaccattat cccagcaaaa gcaaataata gtgttggtgt
     5281 aaacattgcc cctccaaaac gctgtatttt cttcatcata taaattactc ccctcctcat
     5341 atttttagtt tatctttggc cttagatatc tattatttta acaggttaat aaatatatac
     5401 aatctttagg gtacattcgg agcttattca cagtcaaata agtttttgca catttttatg
     5461 ttcaaattct cataaaaaaa aagaatgact acaatttgca atcaatccct tctgtaatca
     5521 ttctatgcct tttataaata ctatatttaa taaataataa ctcaatcaat ataaaatata
     5581 acattgtcaa tttataacta gggtgcattt gtaaaaatga gatattagtc gctgaaatat
     5641 aaattgattc atcacttaaa tcagtcaatg tattattttt aaactctgta attgaaatta
     5701 ttttagttcc tattaatttt aatcttctag caatattaag tgtattctca ttctcaccac
     5761 ttagggatat aataatcata acatcatttt tactgacaat attgtaaaaa gaatcatcaa
     5821 atgttcttcc ctcaaaatca tatattattt cttgacatga taagaacatt ctctttatct
     5881 gttttgcaac cacattttga atatcacctg aaccataagc gtatattgtt cttgattcat
     5941 aaagaagttt acacaatgca tcataatctt tatttttttg atcttcaaca tacttaatta
     6001 ttgaattaca tgcaatatca aatgactgat tttctatgac atgaaccttt tctgattccc
     6061 agcttaataa tactttaaac tcactgaaac cttttaaatc taatttttta acaaatcttg
     6121 taatagttgc acgagacaca ttacatttca tagcaagttc atttattgac atcttactac
     6181 aatgtttttt atttgcagtt atatatttcc atattaataa atctgtatca ttcaatttat
     6241 tataatttaa aaaaatcatt tcttctaatc tcataaacta ttaccccctt taattaaaaa
     6301 tcgtcataaa tattatacca atattcaaca ttaaattaca gcgtattctt atatttacta
     6361 tttagcccat aaaattgact ataaaaaata ctgctataat tgatgtttaa aaaaatgtag
     6421 ttttaatttt attcttttat ctaataatta atattttcaa gttctagtgc ttttcttata
     6481 caattatcat ctttaaaatc agcagtcctt ttacaatctt ttactttaaa ttctggaata
     6541 gttttaaatt cctcatcaca tattccattc ttatttaaat acatacaact tgccattcta
     6601 actattagac cactattttt taaattaaac aatacagggt ctatacaccc tccatctccg
     6661 ccagtagttt caccaactaa agtcgcaaat ttattttctt tacaaaacat tgcaaatgac
     6721 tctgatgatg aatatatttt tctatcaaca agtaagtata tattaccctt aaattttgaa
     6781 tcatctgtag attttatagt ataggaagta tcttcaaaat cattaaaatc ttttataatt
     6841 tctttaggtg cattttcctt tacacttgct ggtaaatttt ctacaggatt taattttata
     6901 tttcttgcat ttgtatattt ttttactatt tcattattat ttctataaat tctataacca
     6961 tttattttga catcattttt tatgagttta gatacgattc cctgccaata cctatcactt
     7021 ccacctaaat tacctcttat atcaataatt aaagctttat gtttttctaa agtgttaata
     7081 taatctccaa ttatttttaa atcattattt atagagccat tctttgatga cattgatggt
     7141 aaatacatat atccaatctg acccttaata acatccttta aaattaattc tttttttaca
     7201 aaaatatcat ctggcatttt tattttaaga ttcatagagt catatctatc cacaactttt
     7261 ttatcattaa gaaaatcata ccaatcatta ttagagtatg atttcttaaa aagttcatat
     7321 cttttcttat tatctattac ttctgtgtgt ttattattta aatcagataa tatagatgac
     7381 agttcttcta taaattcttc atcattttca gtgtttttta ttcgttttgt atattcatcc
     7441 ttatttgcta accaatcaat attattaagc ctcttattta cacctagata tggatatcct
     7501 gtctttacaa cattatacac ataattaaag tcatccacct tttcctcttc agacaactga
     7561 ttcttttgag aataagtaga attacctaat aatattataa atgctaaaat aattactaaa
     7621 aataatactt tctttttcat gtaattctca tccactttca gattttatat tttaatacat
     7681 tgtatcatta ttttattatc acctaaacag ttaaaagaaa ttcttgaaga tatattccac
     7741 tattattctc tttaaaaatt tttatttgta ttttcaaaat tatttcatga aagttataaa
     7801 ataacaatta tattcaaata tctattttac tgctatatcc tgaaagtaaa ttcttatcag
     7861 tgatatacta aaaaagatat gctaaaaaat ttatccatta taatgactta aatttagata
     7921 ctttactgat ttttatttat tagttgagaa agatatt
//
LOCUS       DAMJYR010000156         7914 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.161, whole genome shotgun sequence.
ACCESSION   DAMJYR010000156 DAMJYR010000000
VERSION     DAMJYR010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7914)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7914)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7914
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.161"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            27..101
                     /locus_tag="PC471_003327"
     tRNA            27..101
                     /locus_tag="PC471_003327"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:59..61,aa:Thr,seq:tgt)
     gene            116..200
                     /locus_tag="PC471_003328"
     tRNA            116..200
                     /locus_tag="PC471_003328"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:150..152,aa:Tyr,seq:gta)
     gene            210..293
                     /locus_tag="PC471_003329"
     tRNA            210..293
                     /locus_tag="PC471_003329"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:244..246,aa:Leu,seq:tag)
     gene            317..391
                     /locus_tag="PC471_003330"
     tRNA            317..391
                     /locus_tag="PC471_003330"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:349..351,aa:Gly,seq:gcc)
     gene            416..492
                     /locus_tag="PC471_003331"
     tRNA            416..492
                     /locus_tag="PC471_003331"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:450..452,aa:Arg,seq:tct)
     gene            502..577
                     /locus_tag="PC471_003332"
     tRNA            502..577
                     /locus_tag="PC471_003332"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:535..537,aa:Gln,seq:ttg)
     gene            586..661
                     /locus_tag="PC471_003333"
     tRNA            586..661
                     /locus_tag="PC471_003333"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:619..621,aa:Lys,seq:ttt)
     gene            664..752
                     /locus_tag="PC471_003334"
     tRNA            664..752
                     /locus_tag="PC471_003334"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:698..700,aa:Ser,seq:tga)
     gene            756..831
                     /locus_tag="PC471_003335"
     tRNA            756..831
                     /locus_tag="PC471_003335"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:789..791,aa:Phe,seq:gaa)
     gene            838..911
                     /locus_tag="PC471_003336"
     tRNA            838..911
                     /locus_tag="PC471_003336"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:872..874,aa:Met,seq:cat)
     gene            926..1002
                     /locus_tag="PC471_003337"
     tRNA            926..1002
                     /locus_tag="PC471_003337"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:960..962,aa:Ile,seq:cat)
     gene            1020..1096
                     /locus_tag="PC471_003338"
     tRNA            1020..1096
                     /locus_tag="PC471_003338"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1054..1056,aa:His,seq:gtg)
     gene            1105..1180
                     /locus_tag="PC471_003339"
     tRNA            1105..1180
                     /locus_tag="PC471_003339"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1138..1140,aa:Lys,seq:ttt)
     gene            1188..1261
                     /locus_tag="PC471_003340"
     tRNA            1188..1261
                     /locus_tag="PC471_003340"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1220..1222,aa:Cys,seq:gca)
     gene            1269..1345
                     /locus_tag="PC471_003341"
     tRNA            1269..1345
                     /locus_tag="PC471_003341"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1303..1305,aa:Arg,seq:acg)
     gene            1358..1433
                     /locus_tag="PC471_003342"
     tRNA            1358..1433
                     /locus_tag="PC471_003342"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1391..1393,aa:Val,seq:tac)
     gene            1509..1922
                     /locus_tag="PC471_003343"
     CDS             1509..1922
                     /locus_tag="PC471_003343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2790430.1"
                     /translation="MYIVIGNEIVDSEELKIIIDKNSEFKVEKDLSKSTKREDVIAYQ
                     LSIDLNYLDSLINEQCNLASLSDEEKFDEYMTLSDELALDLEELMPKYTIINARAYKL
                     DEVDGIVKIILAVAYADLGHLKLSDVVKRLSRQVD"
     gene            2231..5662
                     /locus_tag="PC471_003344"
     CDS             2231..5662
                     /locus_tag="PC471_003344"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HDF2790431.1"
                     /translation="MLKKFNKILVANRGEIAIRIFRACSELGIKSVGIYSKEDKYGLF
                     RTKADESYLIGEGKGPIDAYLDMDGIIDLAKRKKVDAIHPGYGFLAENAEFARKCEEN
                     GITFIGPSSKVMNMMGDKINSKKIAKEVNVQTIPGVEKAIRSTEEAKEVANKIGYPVM
                     IKASNGGGGRGMRIVHREEDLELEYETACSESRKAFGEDIIFIEKYIADPKHIEVQIL
                     GDNYGNIVHLYERDCSVQRRHQKIIEYAPAFSLDDKVRKEICEDAVKLSKHVGYSNAG
                     TLEFLVDANGGHYFIEMNTRVQVEHTVTEMVTGIDIVQSQILIAQGYSLDSEEINIKS
                     QDDVEIRGYSIQCRITTEDPKNKFMPDTGKIQVYRTGSGFGIRLDGGNGFTGANISPH
                     YDSLLVKTISWDRTFQGAINKTIRSIKELRVRGVKTNVGFLVNVLNNPIFSNGKCSTK
                     FIDENPDLFEITESKDRGTKLLQFIGDVIVNDNACKEKPLFDALHDPRMDKDGSKSEG
                     SKILFDKLGKSAYIEKIKNDKKLLLTDTTMRDAHQSLLATRIRTYDLLKAAKPTEKYQ
                     KDLFSLEMWGGATYDVAYRFLKESPWRRLQKLREEIPSIMFQMLLRASNGVGYKNYPD
                     NVIEEFTRESARQGIDVFRIFDSLNWVENMKPSINTALETGKIVEATMCYTGDILDKT
                     KTKYNLEYYIKMAQELESLGADIIAIKDMSGLLKPYSAYTLVKELKKNVKAPIHLHTH
                     DTSGNGVATCLMASEAGVDIIDAALESMAGLTSQPSLNAIVEALKNTERDTGIDLFGY
                     DELGKYYKDLRKVYNKFESDLTNSCAEIYNFEIPGGQYTNLKPQADSLGLVNRFDEVK
                     EKYKEANEVVGDIIKVTPSSKVVGDLAIFMTKNKLDKDNIIEEGKNLSFPDSVVDYCK
                     GMIGQPEGGIPKDLQEVVLKGEEAITVRPGSLLPAEDFDEIAKYLNEKYDINANIRNV
                     ISYALYPKVYEDYIKHLQHYNDISKLESDVFFYGLNKNEECEVEIEEGKVLTIRLVEI
                     GEVKENGFRTIGFELNGMVREVEIKDKNFSGKINNVEKADMNDPLQIGASIPGKVIKI
                     MVKEEDEVKANQPLIVIEAMKMETIIVAKTDGVIKSIKVKEDDMVEDKQLLMIMK"
     gene            5904..7832
                     /locus_tag="PC471_003345"
     CDS             5904..7832
                     /locus_tag="PC471_003345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HDF2790432.1"
                     /translation="MKVYDSKMLRNVAVLGHSGCGKTNLIETIAYTANTNKIPKLTDK
                     VNMTYSMGLIPIEYNDYKFNLLDTPGYFDFSGDVVSSLRASDAAIIVIDATAPIQVGT
                     EKSLELTESIPKIMFINKIDNEKARYKDAIAMLREKYNNKIVPMISPIYKDKNFVKLH
                     NVFENIDDLEGEFKEQAMSVKEALMELIAETDDQILDKYFNGEELTTEEIQKGIIIGI
                     QRGDIIPVICGSTINNIGTKEILDTISSYLEPIFTEESKPFRGLVFKTMVDPFVGKMS
                     YIKITEGVLSKDKDVFNINKNVKEKIANIYTLRNSELVEIEKAKAGDIVVITKVNSLK
                     TGDTISADKDAEALEKIDFPKPQIYYAVTPKNKGDEEKVASVLNKLVEEDPTLHWYRN
                     TETKQALLGGQGELHIKTIKNKMKDKFGVDVELNDLKVPYRETIKGTADVQGKHKKQS
                     GGHGQYGDVKIRFERCESDFEFTEEIFGGSVPKQYIPAVEKGLKDSMQKGILAGYPVT
                     NIKATLYDGSYHDVDSSEMAFKMAASAAFKKGMEEAHPILLEPIMKLKITVPEEYMGD
                     VMGDINKRRGKIFGMEPDDKGKQIIFAEAPQAETFKYAIDLRAMTQGRGYFEMELERY
                     GEVPSQFAEKIIGLATAK"
BASE COUNT         3098 a         1011 c         1592 g         2213 t
ORIGIN      
        1 ccccttccag gtcgccaaaa taaaatgctg gtgtggctca acggtagagc agctgacttg
       61 taatcagcag gttgtaggtt cgattcctat caccagctcc atataaaatt aatacggagg
      121 atttcccgag cggccaaagg gggcagactg taaatctgtt gtcatcgact tcggtggttc
      181 gaatccacca tcctccacca aaaaaatgcg cggatgtggc ggaattggca gacgcactag
      241 acttaggatc tagcgccatt ggcgtggggg ttcgactccc ttcatccgca ccatttttat
      301 ttaatttaat aaacgagcgg gaatagttca gtggtagagc gcaaccttgc caaggttgaa
      361 gtcgcgagtt cgaatctcgt ttcccgctcc aaattttatg tttatgttgt aatgtgggtg
      421 catagctcag ctggatagag caacgccctt ctaaggcgtg tgtccggggt tcgaatccct
      481 gtgcgctcac cacatatata ttggggatta gccaagtcgg taaggcacat gactttgact
      541 catgtatgcg taggttcgag tcctgcatcc ccagccaaac agtatggtct attagctcag
      601 tcggtagagc acctgacttt taatcagggt gtcccgcgtt cgagtcgcgg atagatcacc
      661 aatggagagg tgtccgagtg gtttaaggag ctggtcttga aaaccagtga cttcgaaagg
      721 ggccgtgggt tcgaatccca ccctctccgc caaatgccca gatagctcag tcggtagagc
      781 aggggactga aaatccccgt gtcggtggtt cgattccgcc tctgggcacc aaaatgtggc
      841 ggtatagctt agttggctag agcgttcggt tcatacccga aaggtcatag gttcgactcc
      901 tattaccgct actgaaattt aatgtgggcc attagctcag ttggttagag cgcccggctc
      961 ataaccggta ggtctggggt tcgagtccct gatggcccac cacattaaat taacttatgg
     1021 tgggtatagc tcagttggtt agagcgccag attgtggctc tggaggtcgt gagttcgact
     1081 ctcattatcc accccaactt atatggtcta ttagctcagt cggtagagca cctgactttt
     1141 aatcagggtg tcccgcgttc gagtcgcgga tagatcacca aaaaggcggc gacatagcca
     1201 agtggtaagg cagtggactg caactccttg atccccagtt cgaatctggg tgtcgcctcc
     1261 aataaaatgc gctcatagct caactggata gagtgtctga ctacgaatca gaaggttagg
     1321 ggttcgagtc cctttgggcg caccatatga atagcgaggg attatagctc agctgggaga
     1381 gcacctgcct tacaagcagg gggtcacagg ttcgagccct gttaatccca ccagtgcaaa
     1441 cctctagtaa aactactaga ggttttattt ttgattgaaa aagtttttat atttaggagg
     1501 taaaaaattt gtatatagta ataggaaatg aaatagttga tagtgaagaa cttaaaataa
     1561 ttatagataa aaatagtgaa tttaaagttg aaaaagattt aagtaaaagc acaaaaagag
     1621 aagatgtaat agcatatcag ttaagtatag atttaaatta cctagattct cttataaatg
     1681 agcaatgtaa tttagccagt ctatctgatg aagagaaatt tgatgaatat atgactttgt
     1741 ctgatgaact tgcactggat ttagaagagt taatgccaaa atatacaata ataaatgcta
     1801 gagcatataa attagacgag gtagatggaa ttgtaaaaat aatactagct gtagcgtatg
     1861 cagatttagg gcacttgaaa ctttctgatg tagtaaagag gctatctaga caagtagatt
     1921 aggatatgaa tatgaactat attaaaaaaa tgtataaaat cttacgtaaa gatgaaaata
     1981 attatgtaaa catagaaaaa aacagtataa aatcaagtca attgtagaaa ttttcacata
     2041 aattcatctg aaattctata aaaaatgcct taattttaat gaggtaaatc gagtttacac
     2101 agtctaaaaa ttacttatat ttataaattt atcttattaa ttaaggaact ttcttaaaca
     2161 tcttcaaaaa taaagtattt tagtctataa tctttaatat tacacaaaca ataaaggaga
     2221 agaatagagt atgcttaaaa agtttaataa aatactagtg gctaatagag gcgaaatagc
     2281 tataagaata tttagagcct gttcagaatt ggggataaaa agtgttggta tatacagtaa
     2341 ggaagataag tatggattgt ttagaacaaa agcggatgaa tcatatttga taggagaagg
     2401 taaagggcca atagatgcat atttagatat ggatggaata atagacctag caaagagaaa
     2461 aaaagttgat gctatacatc caggatatgg ttttctagcg gagaatgcag aatttgctcg
     2521 taaatgtgaa gagaatggaa ttacttttat aggaccatca tctaaggtaa tgaatatgat
     2581 gggggataag attaactcta aaaaaatcgc aaaagaagtt aatgttcaaa ctataccagg
     2641 agtagaaaaa gcaataagaa gtactgaaga agcaaaggaa gttgcaaaca aaataggata
     2701 tcctgttatg ataaaagcat ctaatggcgg tggcggtaga ggtatgagaa ttgtacatcg
     2761 cgaagaagac ttagagcttg aatatgaaac tgcttgtagt gaatcaagaa aagcttttgg
     2821 agaagatatt atatttatag aaaaatatat agctgatcca aaacatatag aagttcaaat
     2881 attaggtgat aattatggaa atatagttca cttatatgaa agagattgtt cagttcaaag
     2941 aagacatcaa aaaataatag aatatgcacc agctttttca ttagatgata aagtaagaaa
     3001 agagatatgt gaagatgcag ttaagttatc aaaacatgtg ggttattcta atgcgggaac
     3061 attagaattt ttagtagatg caaatggtgg gcattatttt atagagatga acacaagagt
     3121 acaggttgaa catactgtaa cagagatggt tacaggaata gatatagttc aaagtcaaat
     3181 actaatagcc caaggttaca gcttagatag tgaagaaatt aatataaaat cacaagatga
     3241 tgtggaaata agaggttact ctatccaatg tagaataact acagaagacc ctaagaataa
     3301 attcatgcct gatacaggta aaattcaagt ttatagaact ggttcaggat ttggtataag
     3361 actagatgga ggaaatggat ttacaggggc aaacataagt cctcattatg atagtttatt
     3421 ggttaagaca atatcttggg atagaacatt ccaaggagca ataaataaaa ctataagatc
     3481 tataaaagaa cttagagtta gaggtgttaa aactaatgta ggattcctag ttaatgtttt
     3541 aaataatcct atattctcaa atggtaaatg tagtactaag tttatagatg aaaatccaga
     3601 tttatttgaa ataacagaaa gtaaagacag aggaacaaaa ttacttcaat ttataggaga
     3661 tgtaattgta aatgataatg cttgtaaaga aaaaccatta tttgatgcat tacatgaccc
     3721 aagaatggat aaggatggtt caaaatcaga aggaagtaaa atacttttcg ataagttagg
     3781 taaatctgca tatatagaaa agataaaaaa tgataagaaa ttactattaa cagatacaac
     3841 aatgagagat gcacatcaat cattattagc aacaagaata agaacatatg acttattaaa
     3901 agctgctaag ccaacagaaa aatatcaaaa ggatttattc tcacttgaaa tgtggggagg
     3961 agctacttat gatgttgcat atagattttt aaaagaatct ccttggcgta gattacaaaa
     4021 gctaagagaa gaaataccaa gcataatgtt ccaaatgttg cttagagcat caaatggagt
     4081 tggatataaa aactatccag ataatgtaat agaagaattt acaagagaat ctgctagaca
     4141 aggtatagat gtatttagaa tatttgattc attaaactgg gttgaaaata tgaaaccatc
     4201 tattaataca gctttagaaa ctggaaaaat tgttgaagca actatgtgtt atacaggtga
     4261 tatactagat aagactaaaa ctaaatacaa tctagaatat tatattaaaa tggcacaaga
     4321 attagaaagc ttgggagcag atataatagc aataaaagat atgtcaggtc ttcttaaacc
     4381 atattcagct tacacattag ttaaagagtt aaagaaaaat gtaaaagcac caatacattt
     4441 acacactcat gatacaagtg gaaatggtgt agcaacttgt ttaatggcgt cagaagctgg
     4501 agtagacata attgatgctg cattagaatc tatggcagga cttacaagtc agccatcact
     4561 taatgctata gtagaggcac ttaagaatac agagagagat acaggaatag atttatttgg
     4621 atatgatgag ttaggaaaat attataagga tttaagaaaa gtttataata aatttgaaag
     4681 tgatttaact aattcttgtg cagaaatata taattttgaa ataccaggag gccaatatac
     4741 aaacttaaaa cctcaagctg atagtttagg attagttaat agatttgatg aagttaaaga
     4801 aaaatataaa gaagctaatg aagttgtagg agatataata aaagtaactc catcttcaaa
     4861 agttgtagga gacttagcta tctttatgac taaaaataaa ttagataaag acaatataat
     4921 agaagaaggt aaaaatttat cattcccaga ttctgttgtg gattactgta aaggtatgat
     4981 tggtcaacca gaaggtggaa taccaaaaga tttacaagag gtagttctaa aaggtgaaga
     5041 agctataaca gtaagaccag gttcattatt accagcagaa gattttgatg aaatagctaa
     5101 atatctaaat gaaaagtatg atataaatgc taatataaga aatgttataa gttatgcttt
     5161 atatccaaaa gtatatgaag attatatcaa acatctacaa cattataatg atatatctaa
     5221 acttgaaagt gatgtattct tctatggatt aaataaaaat gaagaatgtg aagtagaaat
     5281 agaagaaggt aaggtattaa caataagatt agttgaaatt ggtgaagtta aggaaaatgg
     5341 atttagaact ataggatttg agttaaatgg aatggttaga gaagtagaaa taaaagataa
     5401 gaatttctct ggaaaaatta ataatgttga aaaagctgat atgaatgacc ctcttcaaat
     5461 tggagcaagc ataccaggaa aagttataaa aataatggtt aaagaagaag atgaagtaaa
     5521 agcaaatcaa ccattaatcg ttatagaggc tatgaagatg gaaactataa tagttgctaa
     5581 gactgatgga gtaattaagt caatcaaagt aaaagaagat gatatggttg aagataaaca
     5641 attattaatg ataatgaagt aatttaatta aaataaatta aaaagcactc tgaaatgttt
     5701 aataaaaaat gttttagagt gtttttattt attttaatta aatatttgtt aaaaaaaaga
     5761 ggacaattac ctccagatgt agaaataaag ctagtaagga aaatgttatc taaaaattct
     5821 aaatatagta aatattttcc ataatatttc ttaggaaata acagcaaaga taaaatacat
     5881 aaatttaaaa ggtgggtgct tatatgaagg tttatgatag taaaatgttg agaaatgtag
     5941 cggtattagg acatagcgga tgtggaaaaa caaatctaat cgaaactata gcttacacag
     6001 caaacactaa taagatacct aaattaacag acaaggtaaa tatgacttat agcatgggat
     6061 taatacctat agagtataat gattataagt ttaacttatt ggatacccca ggatattttg
     6121 actttagcgg agacgttgtg tcatctctta gagcaagtga tgcagcaata atagtaatag
     6181 atgcaacagc gcctattcaa gttggtacag agaaatcatt agaactaact gagagtattc
     6241 ctaagataat gtttataaat aaaatagata atgaaaaggc aagatacaaa gatgcaatag
     6301 caatgttaag agaaaaatac aataataaga tagttcctat gataagtcct atatacaaag
     6361 ataaaaactt tgtaaagtta cataatgttt ttgaaaacat agatgattta gaaggtgaat
     6421 ttaaggaaca agctatgagt gttaaagaag cattaatgga acttatagca gaaactgatg
     6481 accaaatatt agataaatat tttaatggag aagaactaac tacagaagag atacaaaaag
     6541 gtattattat aggtatacaa agaggtgata ttatacctgt aatatgtggt tcaactataa
     6601 ataatatagg cacaaaggaa atattagata caatatcaag ttatttagaa cctatcttta
     6661 cagaggaaag caaaccattt aggggattag tatttaaaac tatggttgac ccatttgttg
     6721 gaaagatgtc atatataaaa attactgagg gtgtactttc taaggataaa gatgttttta
     6781 atataaataa aaatgtaaaa gaaaaaatag ctaatatata tacattgaga aatagtgaat
     6841 tagtagaaat agaaaaagct aaagctggtg atatagtagt tataactaaa gttaattctc
     6901 taaaaacagg agacactata tctgcagata aagatgcaga agctttagaa aagatagatt
     6961 ttcctaaacc acaaatatat tatgcagtaa ctcctaagaa taaaggtgat gaagaaaaag
     7021 tagcatctgt attaaataaa ctagtagaag aggaccctac attacattgg tatagaaata
     7081 cagagacaaa acaagcactt ttaggaggtc aaggagaact tcatataaaa acaattaaaa
     7141 ataaaatgaa agataagttt ggtgttgatg ttgaattgaa tgaccttaaa gttccttata
     7201 gagaaactat aaaaggaaca gctgatgtac aaggaaaaca taaaaagcaa tcaggtggtc
     7261 atggtcaata tggagacgta aaaataagat ttgaaagatg tgaatctgat tttgaattta
     7321 cagaagaaat atttggtggt tctgtaccaa aacaatatat accagcagtt gaaaaaggtt
     7381 taaaagactc tatgcaaaaa ggtatattag caggatatcc tgttacaaac ataaaagcta
     7441 cgctttatga tggttcatac catgatgtag actcttctga aatggccttt aaaatggcag
     7501 caagtgcagc ctttaaaaaa ggtatggaag aagcacaccc aatactacta gaacctataa
     7561 tgaaacttaa gataactgtt ccagaagaat atatgggaga tgtcatgggt gatataaata
     7621 agagaagagg aaaaatattt ggaatggaac cagatgacaa agggaaacaa ataatatttg
     7681 cagaagctcc acaagcagag acatttaaat atgcaataga tttaagagca atgacacaag
     7741 gtagaggata ctttgaaatg gaattagaga gatatggaga ggttccatca cagtttgcag
     7801 aaaaaataat tgggttagct actgcaaagt aatgaacaaa ataaatagaa aaataattaa
     7861 tatatattat taaaaatatt tttagggttt taactaggta taggtttatg ttag
//
LOCUS       DAMJYR010000157         7814 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.162, whole genome shotgun sequence.
ACCESSION   DAMJYR010000157 DAMJYR010000000
VERSION     DAMJYR010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7814)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7814)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7814
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.162"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(318..980)
                     /locus_tag="PC471_003346"
                     /pseudo
     CDS             complement(318..980)
                     /locus_tag="PC471_003346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016440163.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
     gene            complement(1140..1676)
                     /locus_tag="PC471_003347"
     CDS             complement(1140..1676)
                     /locus_tag="PC471_003347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrpB family protein"
                     /protein_id="HDF2790433.1"
                     /translation="MIGLKCGMVKLLEHQMIWDKSAKDVIILLKSIWDKTAIDIQHIG
                     STSIPSISAKPIIDIVVGVASLEEAKLYLERLEQCGIVFRGQDVPKQLLFAMGDFEKN
                     TRTHHIHVVEWNSVAWNNYINFRDYLNTFTDKAKLYDSHKQKLALQFEDNRKIYTQRK
                     QELINILLEEARLWRINT"
     gene            complement(1749..2162)
                     /locus_tag="PC471_003348"
     CDS             complement(1749..2162)
                     /locus_tag="PC471_003348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003507530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3795 domain-containing protein"
                     /protein_id="HDF2790434.1"
                     /translation="MKMPKESIDTIMFAPCGMNCMVCYKHCYHKKPCAGCLNGDTGKP
                     EHCRKCKIKDCVCQKGLFYCFECFNFPCKLIKNLEKSYNKRYQASLMENSKFVQQYGL
                     EKFMQKQKERYTCSKCGGIISIHDRECSECQEKVK"
     gene            2628..3182
                     /locus_tag="PC471_003349"
     CDS             2628..3182
                     /locus_tag="PC471_003349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid stabilization protein"
                     /protein_id="HDF2790435.1"
                     /translation="MDEKILELLQEMQGNISNMQGSISGIEGTISDMQSNINGIEDTI
                     SGMQGTISDMQGSISSMNGTISDMQGSISSMDGTINSMQDSITDMQVEIKEVNSRLDN
                     MEPDIKDLKTGQVETSSRLDTIQSDVTEIKSDVKINRNTLDTVIKHTSSLVKDTSDIK
                     NDLNNIEIVTSKNSNDIAKLKSVK"
     gene            complement(3790..3969)
                     /locus_tag="PC471_003350"
     CDS             complement(3790..3969)
                     /locus_tag="PC471_003350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDF2790436.1"
                     /translation="MLQTGKQGADKKYLDNMFFVKGYVEELLFLDNSYDIVFSCIVFH
                     HCTNVQNIFCIFWKW"
     gene            complement(4195..4728)
                     /locus_tag="PC471_003351"
     CDS             complement(4195..4728)
                     /locus_tag="PC471_003351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HDF2790437.1"
                     /translation="MQYISYYHSPIGNILLASDNIGLTGLWFENQKYYAYNLDQEHQE
                     KELPIFEQTKKWLDIYFSGKEPNFTPSLHMVGTPFQTTVWKILQQIPYGKTITYGEIA
                     YKVAQQKGISKMSAQAIGGAVGHNGISIIIPCHRVVGTNGSLTGYAGGIDRKIKLLSL
                     EKVSVQHYFIPKKGSAL"
     gene            complement(6476..7048)
                     /locus_tag="PC471_003352"
     CDS             complement(6476..7048)
                     /locus_tag="PC471_003352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HDF2790438.1"
                     /translation="MWIYQKTLEHPVNIRQADPRMAKYIMTQLGGPNGELAAATRYLQ
                     QRYTMPTGKSRALLTDIGTEEMAHVEIISSVLYQLIGNCTPEELKAAGLGSNYANFGH
                     GLQPVDSNGVNFTTSYINVFGDSVTDLHEDMAAEQKALATYYQLINLTDDPDLKDILR
                     FLGEREVVHYQRFGEALMDVYEFTECKHQF"
     gene            complement(7110..7373)
                     /locus_tag="PC471_003353"
     CDS             complement(7110..7373)
                     /locus_tag="PC471_003353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat protein CotJB"
                     /protein_id="HDF2790439.1"
                     /translation="MSLNRRELLERISEYQFACIELNLYLDNNPRDKKALDSYNRYCD
                     KFTQAVCDYESKYGALTNFGYESSEYPWSWISEPWPWDKSFYK"
     gene            complement(7373..7594)
                     /locus_tag="PC471_003354"
     CDS             complement(7373..7594)
                     /locus_tag="PC471_003354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat associated protein CotJA"
                     /protein_id="HDF2790440.1"
                     /translation="MGYLKEDKQDYCCEFARPYINRQIYDDTYNPKTALKRGTLFPEL
                     DLIESNNYNDWLYANPKNRVRAKKGGNMY"
BASE COUNT         2706 a         1334 c          930 g         2844 t
ORIGIN      
        1 atagcttgta gtgtacttcc ctcaagagga atagtcgttg aagtttctcc tattcggagt
       61 ttacctgctg attgcccatt ttaaagtact tagggtttaa ccttatacca tctaaccaat
      121 tttttctact ttcgtaactt tcacacttag gtttatttca tccttatgtt ttagtttggt
      181 tagctttagg ggtttccagc aattcaagta gtattggata ggttctatcc tatctctaca
      241 tataggtttc cctatatgct gactatttta gctactaact catgactaaa gtcacaagtg
      301 ttcgtagcta ttttttaatc aaactggaat acagactcca ttcctaggaa attggctgaa
      361 atatagtctg ctgtcaaaga tgctgtcatc acagaaatta aaacagatac tgaaaagttt
      421 ttatggactt cttcaagtga aaacataaca cccgctagtg gtgcatgaaa ggctgctgat
      481 aatccagcac ttgccccaca ggttaacagg aatttttcct ctgtcttccc tcggtcaaga
      541 gctctagaaa tacctttacc tgccatagca ccaagctgaa tggaaggacc ttcccttccc
      601 aaagcaagac cacccatgag tgataagaat cctccaataa attttgttgg caacactcgc
      661 caccatactt gttttatttt ccctgtcatt tccccctcaa gctgagggat accacttcca
      721 gaaatcattg gctcccagtc caccaacttt gatacaatcc acgcaagtac tgcaagtact
      781 gcaaaccaag ctattatttt tattgtagaa ccttctacaa attttaaaat ttggctaagc
      841 caagtaccag cctgccctaa aagtatcctg tataaaagca caatcaaacc agccacagcc
      901 ccaaccataa gtccttcacc aatcaaaatt acctgaaagt gctctgcccg tttgataata
      961 tgagatgtat cctttttcat actattcctc cttcatccaa taattctaca caatctgaat
     1021 ctccaatgac taaagtcatg agtgtgtaca gatatttttt aatcaacaaa aatagatttt
     1081 tacttactaa atataagaaa ataattgctc aatttctcaa taaatgtgaa tttattcttt
     1141 taggtattta ttctccacag tcgtgcttct tccagcaaaa tatttattaa ttcttgcttt
     1201 ctctgtgtat agattttacg attgtcctca aactgtaatg ctaatttttg tttatgacta
     1261 tcatagagtt ttgctttgtc tgtaaaagta tttagataat ctcgaaaatt gatataatta
     1321 ttccatgcaa cactattcca ttcaacaaca tgaatatggt gagtacgagt atttttctca
     1381 aaatctccca tagcaaaaag taattgtttt ggtacatcct gacctcgaaa aacgattcca
     1441 cattgctcta acctttcgag gtataattta gcttcctcta aagaagcaac acctacaaca
     1501 atatcaataa taggctttgc gctaatactg ggaatagaag tgcttccaat gtgttgaata
     1561 tcaatagcag tcttatccca tatacttttt aataatataa tgacatcttt tgcactttta
     1621 tcccatatca tctgatgttc caataattta accataccac attttaaacc tatcactata
     1681 taccacctca atataatcca cctttatata tattacaatt ttttctatct tgataaatag
     1741 tgatttagct atttcacttt ttcttgacac tcgctacatt ccctatcatg aatagatata
     1801 attcctccac atttagaaca agtatatctc tccttctgct tttgcataaa cttctctaaa
     1861 ccgtactgct gaacaaattt actattttcc ataaggcttg cttgatatct cttgttataa
     1921 cttttttcaa gattttttat gagtttacaa ggaaaattga aacattcaaa acaataaaac
     1981 aacccttttt gacagacaca atcctttatt ttgcacttac ggcagtgttc tggctttcct
     2041 gtatcgccgt tcaaacaacc agcacatggc tttttgtgat aacaatgctt atagcaaacc
     2101 atacaattca ttccacaagg agcaaacata atcgtatcaa tactctcttt tggcattttc
     2161 ataacatttt actcctttca aaaatccaat ttgtcgaatt gttatatatt catattgtaa
     2221 cataaaaaat agctaaaaaa ttcatgcact ttcattgcca ataaattttc aaggaaatat
     2281 gaaagtttaa aactaagaat aaaaagagat aaaaaggaag ctactaatca aaatattcaa
     2341 acacaagtaa tcaatataaa taacttaagg tatatttaaa agtatatgat attaccagca
     2401 gttttaaata caaatataac cacacatgcc tcaaaaacac ttattacagt atcttagaaa
     2461 cttagacaat acataagatt cattaataaa aaagttctca atatagatat atttaaccat
     2521 gttttgaata gtagataatc atatgattaa aaattaaata tgacatagcg tttgtggaat
     2581 ataacaccta aattatgtta taataataaa gcaggagtgt gatagtgatg gatgaaaaaa
     2641 tattagagtt attacaggaa atgcaaggta atattagtaa tatgcaaggt agtatcagtg
     2701 gtatcgaagg cactattagt gatatgcaaa gcaatattaa tggtatcgaa gacactatta
     2761 gtggcatgca aggcactatt agcgatatgc aaggcagtat cagtagtatg aatggcacta
     2821 ttagcgatat gcaaggcagt atcagtagta tggatggcac tattaatagt atgcaagaca
     2881 gtattactga tatgcaagtt gaaattaaag aagtcaatag taggttagac aatatggaac
     2941 ctgatattaa agacttaaaa actggacaag ttgaaactag tagtagactt gatactattc
     3001 agagtgatgt tacagaaatt aagtcagatg taaaaattaa cagaaataca ttagatacag
     3061 tgattaaaca tacctcttca ttagtaaaag atacatcaga tattaaaaat gatttaaata
     3121 atatagagat agtcacatct aagaattcga atgatattgc taaattaaaa tcagttaaat
     3181 aatatactat agtaaaaaat acctgaaaat ttcacatact tctttaaagt tataccaaat
     3241 tttatacaga aatactgatt atccgacaac acactttact tataaataat ttattaaaaa
     3301 aatactcatt ttatcctaaa attaacacga ctaaatgagt cttgatataa taataaaaaa
     3361 taagggagat tgaacactaa aaagttgtgt atctccctta tattctataa tatagttata
     3421 taatttttca gcctaaaata aaagtccatc tttgattaaa acacatcccc ttatcactta
     3481 taaaaggata aaatcttgtt ttcatttttc catttatatc ttcttgctta agagtaataa
     3541 ttttttcttt tgatacttta agtgattggt taaatttaac caaacgacta agttttgttc
     3601 aacatctatt tttcatattt tccaatactt aatctatttt ttataaataa ttcttttatt
     3661 tctaactcac ttacattttt tacataaaac gaagcatata aatttcaacc ttatcttgta
     3721 tggttcttaa tacttaatga gtagcttaag tatcaatcat tataaatttt tcccttacat
     3781 ttaacactct taccatttcc aaaatataca aaatatattt tgaacatttg tacaatgatg
     3841 aaatacaata catgaaaata caatatcata actgttgtct aaaaatagaa gttcttcaac
     3901 ataacctttc acaaaaaaca tattatctaa gtattttttg tctgctcctt gttttcctgt
     3961 ttgtagcata taaattactg catctaaaca tataactact cacacgaaaa aagaaaataa
     4021 acttccaaaa tacaagctct aaaaactacc tctaaagttg tataatgttt actaggaata
     4081 aggttgtata acatatagtt taaataatca ttttttaaaa ttgcaagtta ttgctttcaa
     4141 aattagatgt ttttatatca aataaatgat taagaatttc taaatttaat ggctttataa
     4201 tgcactccct ttctttggga taaaataatg ttgcacagat actttttcta aagataacaa
     4261 ttttattttt ctatcaattc caccagcata cccagtcaaa ctcccatttg ttcctacaac
     4321 acgatgacat ggaataataa tagagattcc attatgccct actgcaccac cgattgcttg
     4381 tgctgacatt ttagatattc ctttttgttg agcaacttta tatgcaattt ctccataagt
     4441 gattgtttta ccatatggta tttgttgtaa aattttccac actgtagtct gaaaaggagt
     4501 acctaccata tggagtgatg gtgtaaaatt gggttctttt cccgaaaaat aaatatctaa
     4561 ccatttcttt gtctgctcga aaattggcaa ttctttttct tgatgttctt ggtcaagatt
     4621 ataagcataa tacttttgat tctcaaacca aagaccagtt aacccaatat tatctgatgc
     4681 taaaagaata tttccaattg gactatggta atagctaata tattgcatac aattcctcct
     4741 tatattctga aaacttcact caaatcatta gacacttctt ttctatctac cattatgtaa
     4801 aaacatacta atataatttc cttctaacta cactttcgtt ataatatcta tcctcatatc
     4861 atattagttt tatatttacc tgcatctgtt catccttttt tccctttccc tttgatgaat
     4921 ataaaattca tacatttaag cttcctactg tattaacttt tatagaagtg tatattataa
     4981 ttttatcact aaataggcca aaaaaatatc gatatagcat caagtaatat cataatactt
     5041 tcaaatggga aaagtaagaa taggctgtaa aaaagaaaag tatttttatg ttagaaatcc
     5101 agtaaaatca ttagtagata gaataacaga aaacgaacaa tttgctattt atcattttac
     5161 acataaatat aattgacgca ataaagtgtt ttctattcta caacgattaa taaggttaga
     5221 aaaaagtaaa aatgaactgt atatgtatga agcattagtt tctgtttctt tcctttggct
     5281 tattatgata aaaaatattg ttttgctacc aataaaacac aaaaatataa ttaatatcca
     5341 tatgcaaaaa attttacgtt atattgaaaa atactattgt tttacattat attgaagggt
     5401 actattgtga aaacttaatt ttggagtact atcaagcagt ggtaatatca ataaatcaga
     5461 gtgctccaga tgatttgaac taagtttaaa tactatacct tataaatatt ttactgagtt
     5521 ttgtttatta aaagcaactc aatttctaaa aggcacaaat gagctaataa gtgacattgc
     5581 tataaaagtt gtttttcatc aaataatcta ttttagtaaa tgttttaaag aaaaaacggg
     5641 atattctcca aaagtgtaca gaaatataaa aaaagagata tgatttttta tttcttttga
     5701 ttatccaaag agtaaataaa aaatattgta aaaaacttac taacaacggc aatgctttga
     5761 actatggtaa tactttgaac ttttgattgg actttttttg ctaaaataat atatttaaat
     5821 agcaaagtaa tctgtttaat caataaaaaa taagagataa aagaaatcaa ctaaaacttt
     5881 tacctcttat tttgatagtt tatcagagct aatatttaca ctctttttat ttttgatact
     5941 tcattaaaaa tgatgcttac tctccattca cttttaattc ttcattaaaa atgatgctta
     6001 ttctccattc acttttaatt cttcattaaa aatgatgctt actctccatt cgcttttaat
     6061 tcttcattaa aaatgatgct tattctccat tcacttttaa ttcttcatta aaaatgatgc
     6121 ttactctcca ttcgctttta attcttcatt agaactatgc ttactctcca ttcactttta
     6181 attcttcatt aaaaatgatg cttactctct gttcactttt aattcttcat taaaaatgat
     6241 gcttattctc cattcacttt taattcttca ttaaaaatga tgcttattct ccattcactt
     6301 ttgattcttc attaaaaatg atgcttattc tccattcact tttaattctt cattaaaaat
     6361 gatgcttatt ctccattcac ttttgattct tcattaaaaa tgatgcttat tctctgttca
     6421 cttttaattc tttattaaaa ctgatgctta ttctccattc actttcaatt cttcattaaa
     6481 actgatgctt gcactctgta aactcataaa catccattaa tgcttcacca aatctttgat
     6541 agtgaactac ttccctctca cccaaaaatc tcaatatatc tttcaaatca gggtcatcag
     6601 ttaaatttat taattgatag tacgtagcca atgctttttg ttcagcagcc atatcctcat
     6661 gtaaatcagt taccgaatcg ccaaagacat taatataact tgtagtaaag tttactccat
     6721 tagaatctac tggctgaaga ccatgtccaa aattagcata attactacca agtccagcag
     6781 ccttaagctc ttctggagta caattgccta ttaattgata taacactgaa gaaattatct
     6841 caacatgagc catttcctct gtacctatat cagttaaaag tgcacgagat tttccagttg
     6901 gcatagtata tctttgttga agatatctag ttgcagctgc caactcacca ttaggtcctc
     6961 ccaactgagt catgatatat tttgccattc tagggtctgc ttgtcttatg ttaactggat
     7021 gttccagtgt tttttgataa atccacatat tatcttactc ctccttaata tatatttaat
     7081 aataaatcta attttaaagt ttaataattt tatttataaa atgatttgtc ccatggccaa
     7141 ggttcagata tccatgacca aggatattca cttgattcat atccaaaatt agttaatgct
     7201 ccatatttag attcataatc acacacagct tgtgtaaatt tatcgcagta tctgttgtat
     7261 gaatcaagag ctttcttatc ccttggattg ttatctaaat ataaatttaa ttctatacag
     7321 gcaaattgat attctgaaat tctctctaaa agttctcttc tatttaatga cattagtaca
     7381 tgttaccccc ttttttagct ctaactctat ttttaggatt tgcatatagc caatcattat
     7441 agttatttga ctctattaaa tctaattcag gaaataaagt acctcttttt aatgctgttt
     7501 ttggattgta tgtgtcatca taaatttgac gattgatgta tggtctagca aattcacaac
     7561 aatagtcttg tttatcctct tttaaatatc ccattaatta tcctccttaa tttttagaat
     7621 tttaaaatag cattattttt aaaaattgta tactggttct atattataac tggtagtttt
     7681 aataggtctt taataagatt aaatgattaa ataagtatct ttttttctta ttttacctct
     7741 aaaatttttc taattaatct ttcataaata tctattttaa ataaataata tagagtttat
     7801 acaatttttt atag
//
LOCUS       DAMJYR010000158         7791 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.163, whole genome shotgun sequence.
ACCESSION   DAMJYR010000158 DAMJYR010000000
VERSION     DAMJYR010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7791)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7791)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7791
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.163"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            1056..2414
                     /locus_tag="PC471_003355"
     CDS             1056..2414
                     /locus_tag="PC471_003355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine/pyrimidine permease"
                     /protein_id="HDF2790441.1"
                     /translation="MKAKSTSKFELDGIPPLKEAIPLGLQHLMAMVIGSCLPAILIAN
                     AAGLNHKMSTLLIQAALVFAGLSTLLQLYPIPLFKGFKLGGGIPVIMGATAMFIGAGV
                     SVASKYGLPVLFGSQIIAGIVIIIIGFGLKKIRFIFTPAVSGTIVACMGLGLFPIAIK
                     NLAGGMGNETFGNPINFIVGISVSLMILGLNKYGKGLFKDASILVSIIFGYILSLILG
                     IVNFSSIQEFTLVALPKPLAFGLDIRLEVVVMFSIIYLVEIADIMGACTLSAVGGLNR
                     QVTDEELSSAVLGNGVLSTIGALFSATPMGMFGQNSAIVSNTKVVSKFVLAIGGIGLF
                     LAGISPLLANLIRTIPPCVVGGATLVIFSTLTTSGLRLVSMDGFNQENSMILGLSMAS
                     GIGFMVAPQVLEKFPKFIETLLADSSVVSGAMVAIIIQALYMVKFPGNKSDKVEDKNK
                     SL"
     gene            2521..3315
                     /locus_tag="PC471_003356"
     CDS             2521..3315
                     /locus_tag="PC471_003356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HDF2790442.1"
                     /translation="MVTIKEYSVPQSLEEAYKILISKKNNVILGGCGFIKLSNKNIGT
                     AIDLKDINLNYIKEDKKNILIGADTSLRSLEIDKTIKNYCSGILSSAVSNIVGVQFRQ
                     GARIGASVFSKYGFSDLIPALLVVGAKVRLYNKGILELSEFLDSELSRDILVEVILPK
                     KDAIAVFDSIRKCTGDFSVLNSAMLKENDTYKIAIGARPQKARLALEASNILNKEKDI
                     DKASIVASKELTYGSNMRASKEYRKDMASALVKRMYNAIEEGMYND"
     gene            3308..5614
                     /locus_tag="PC471_003357"
     CDS             3308..5614
                     /locus_tag="PC471_003357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HDF2790443.1"
                     /translation="MINKGIRKIDSTAIVTGKPLYTEDLIMHKDVLTLKLLRSPHAHA
                     KIKNIDTSKAEKIPGVVGIYTYKDVPQVRYCVPGQTEPEASPYDRVLLEDTVRFIGDE
                     VALVAAEDEKTALKALKMIKVDYEVLKPVFDAREAEGNEVVIHPEKDIVEPYPFGLDA
                     KNNIVSKEFFEFGDVEAKLKESDFVVESSYKTQSQSHCMMETHRSYSYKDINDRIVVV
                     SSLQSPHNVKRILRKVLGIPSSKIRVIKPRIGGGFGGKNIAATDIFTAFVTMKTGRPA
                     KFVFTRSETFVGSNSRHEMFFDVKVGADKEGNLKAIELKSLNNTGAYGGNGVSVSSES
                     GHNTLPIYNDIDAVRFNARTVYTNRLPAGALRGYGATQGIFAIDSAIDELAHKIGMDP
                     LEFRIKNIIDRYTNGKAAEDEILSCELRQCIEKGKKLINWDDKYPCKDIGNNKVRAVG
                     MAVANHGSGIPRVDMATVTIRMDEDGTYKILSGLADLGQGADTIQAQIAAEALNTTVD
                     RISIYTGDSDLCPYDTGAFASCTTTVTGNATIKTANKLKDILLSVAESKLGFKKEDLE
                     LGKDRVFVKNDNSKFVLLEELGRESVGGIGFASDDVPVVLEATESFGMLKKIKPFIAG
                     FAEIELDKNTGEYKVINFACVPDCGTVINPTLARIQVEGGVMMGIGFAMYEDPIFDKD
                     GRLLTDSFLQYKVPTKKEVGNILVDFAENFDPGGPYGAKSLGEIVIHTPAPAIANAIF
                     NATGVRLRELPMTFEKVYMGMKNNNKQL"
     gene            5753..7030
                     /locus_tag="PC471_003358"
     CDS             5753..7030
                     /locus_tag="PC471_003358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDF2790444.1"
                     /translation="MSNRIKNIHYGTQRQQLKNLTKKEYQALREMCFLAKNLYNVGTY
                     NVRQHYFNQGEHLNYEANYHVCKENENYKLLNSNIAQQILKEVDSSFRSFFGLIKLAK
                     EGKYDFRGIKSPKYLDKEGFFSIIIGQIRIKEDGILNVPMSPAFKKLYGKVSIKVPQN
                     ILEKKIKEIRIIPKHKARFFEIQYCYEIPKSEEVSNQKNALAIDLGLENLCTCVTNLG
                     DSFIIDGKRLKSVNQWANKQNDKIEALKLKKNITAITRKQFKVWNKRNNQVKDYVNKT
                     CFYIIDYCVKNNIGNLIVGYNAKLEKSDNMERKIYRNFIRIPFGEIKGKLEYLCKIKN
                     INFIRQEESYTSKSDFFADDFLPDINVDNVKKYDFKGERITRGQYKSSVGIIINADVN
                     ASFNILKKSQVVNLSNLQNNPQILKQPQRVRIS"
     gene            7213..>7791
                     /gene="yqeC"
                     /locus_tag="PC471_003359"
     CDS             7213..>7791
                     /gene="yqeC"
                     /locus_tag="PC471_003359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium-dependent hydroxylase
                     accessory protein YqeC"
                     /protein_id="HDF2790445.1"
                     /translation="MELSNIIEKNEIITVVGAGGKTSFINYFANFYRNKLKVLLTTTT
                     KIYVPNDYDNIIITIDGTVIPSICHGITVCGSYINNENKLVSIDSSILDEIVDQFDLV
                     LIEGDGSKRKKLKGWNAKEPVVYHRTTKTIGILDITSFGMNINEENIHRVEIFKKIAN
                     LDTSSINSSSTVSIENLKNIVLNPNGLFKNYSG"
BASE COUNT         3059 a          846 c         1445 g         2441 t
ORIGIN      
        1 ttgctctata aaaattataa atagtaatat gaataaaatt tttactataa aaataatatt
       61 actattttgc ttatagatat atatatctat aaatagatag aaacttgtat attttatgtt
      121 gcagatacaa atatagtaaa ggagagtaca ttggtacaac tgagatacat agcttattaa
      181 gtttttctgt cttatgagaa ttatattata aaatattcat aaatattttg agtttaaatt
      241 aaataattat tttatgctta gattaattga tatttaatta atttaataac aaaaacagtg
      301 aaagagtaaa taatattatt tacagggggt aaagcaaagt atgttaaata aattgtactt
      361 ttggccccgt acatagagaa atctatgtag gacacaactt taaaaccagt aaatcctaaa
      421 gccttgcaac tacaataaaa gatagaaaga aacttttatg aatgtggtga aaaccagaaa
      481 caatagtgag gatggtatat ggtgagagcc taagtactta aaaatggaca tttggtcgcc
      541 aagcctagaa ataggaaggt caaacgacta tctcgagaga gagtacacta taagcgattg
      601 atagtggaaa cgggttgctc ttagcaggta atgctgaaga tgaagaaata gtctacgctc
      661 atatgaaagt atgaggtcac tggatttgaa gtgacatcta taaagtagcg tttataggta
      721 aatgtttaaa acttgaaaca tgtaaatact tatgaaaact tataacttat aatgattaag
      781 attgaaagat ttaaattgaa ttaaatttac tataaaaaat taaaaattca tagctataat
      841 actcttatat ttgtaaagca tggtatataa tgtatacaaa attaaagaat acgtgtattt
      901 gttaataatt aaatttattg catctgtttt ttaagactaa aaacttaaat atatgttgga
      961 gtatagttga tatttatgaa catcattaat ttaatatatt agatttttaa gtatttatat
     1021 aattcaataa gttttatgta acgggagttt ttattatgaa agcaaaaagt acatcaaaat
     1081 ttgaattaga cggaatacca ccactaaagg aagcaatacc tttagggtta caacacttaa
     1141 tggcaatggt aataggtagt tgtttaccag cgatattgat tgcaaatgca gctggactta
     1201 atcataagat gtcaacatta ttgattcaag ccgcattagt ttttgcagga ttatcaactt
     1261 tattacaatt atatccaata cctttgttta aaggttttaa attaggagga ggaataccag
     1321 ttataatggg agctacagct atgtttatag gagctggggt atctgttgca agtaagtatg
     1381 gactaccagt attatttggc tctcaaataa tagctggtat agtaataatt ataattggtt
     1441 ttggtcttaa aaaaatacga tttatattta ctccagctgt atcaggaaca atagttgcat
     1501 gtatgggtct tggacttttt ccaatagcta taaaaaactt agctgggggt atgggcaatg
     1561 agacttttgg gaatccaatt aattttatag ttggaatatc tgtatctctt atgatattag
     1621 gattaaataa gtatggaaaa ggattattta aagatgcatc aatattagtt agcataattt
     1681 ttggatacat tttatcatta attttgggta tagtaaattt ttcttcaatt caggagttta
     1741 ctttggttgc acttccaaaa ccacttgctt ttgggctaga tataagactt gaagttgttg
     1801 taatgttttc aataatatat ttagttgaga tagctgatat aatgggtgct tgtacacttt
     1861 ctgcggtagg agggcttaat agacaagtaa cagatgaaga actttcttct gctgttttag
     1921 gaaatggagt actctctaca ataggtgcat tattttctgc aactccaatg gggatgtttg
     1981 gacaaaattc tgcaatcgtt tccaatacaa aggttgtaag taaatttgta ctagctattg
     2041 gaggaattgg attatttttg gcaggaatat cgcctttatt agctaattta ataagaacaa
     2101 ttcctccctg tgtggttgga ggagcaacac tcgttatatt ttcaactctt acaacatctg
     2161 gtttaagact tgttagtatg gatggattta atcaagaaaa tagcatgata ttaggtcttt
     2221 ctatggcttc tggtattgga ttcatggtag ctcctcaagt tcttgaaaaa tttccaaaat
     2281 ttatagagac attacttgca gattcaagtg tagtttcagg agcaatggta gccataataa
     2341 ttcaagcttt atatatggta aaatttcctg gaaataaaag tgataaggtt gaggataaaa
     2401 ataaaagttt ataatgtaaa ggtttaatat atgtgaatta aatatactac aattataaat
     2461 ataaataaaa gatttattaa aaacttttgt gttttaataa gttaatgatg gggggataag
     2521 atggttacta taaaggaata ttcagttcca cagagtttag aagaagcata taaaatttta
     2581 atttctaaaa aaaataatgt tattttaggt ggatgtggat ttataaaatt aagtaataaa
     2641 aatataggaa cagcgataga tttaaaagat ataaatctaa attatataaa agaggataaa
     2701 aaaaatattt tgataggtgc agatacttca cttagaagtt tagaaattga taaaactata
     2761 aaaaattact gtagtggaat actttcaagc gctgtttcta atatagttgg tgttcaattt
     2821 agacaagggg ctagaattgg tgcaagtgta ttttcaaagt atggcttttc tgacttaata
     2881 ccagcacttt tggttgttgg agcgaaggtg agattataca ataaaggaat actagagtta
     2941 agtgagtttt tggatagtga attaagtagg gatatattgg tcgaagtaat actaccaaaa
     3001 aaagatgcaa tagctgtttt tgactcaata agaaaatgca caggagattt ttctgtatta
     3061 aattcagcaa tgctaaaaga aaatgatact tacaaaatag caattggggc aagacctcaa
     3121 aaagctagat tagcacttga agcgagtaat atattaaata aagaaaaaga tatagataag
     3181 gcatctattg ttgctagtaa ggaacttact tatggaagta atatgagagc tagtaaagag
     3241 tacagaaagg atatggcaag tgcattagtt aaaaggatgt ataatgctat tgaggaggga
     3301 atgtataatg attaataagg ggataagaaa gatagatagt acagcaattg tcacaggaaa
     3361 acctctatat actgaagacc tcattatgca taaagatgtt ttgacattaa aactacttag
     3421 aagtccacat gctcatgcta agataaagaa tatagataca tcaaaagccg aaaaaattcc
     3481 aggggttgtg ggtatttata cgtataaaga tgtaccacag gtcagatatt gtgttccagg
     3541 tcaaacagaa ccagaagcat caccttatga tagagtttta cttgaagata cagttagatt
     3601 tattggagat gaagtagctt tagtggctgc tgaagatgag aaaacagcac ttaaagcctt
     3661 aaaaatgata aaggtagatt atgaggtttt aaagcctgtg tttgatgcaa gagaagcaga
     3721 aggaaatgaa gttgttatac atccagaaaa agacatagta gagccatacc cttttggatt
     3781 ggatgcaaaa aataatatag tatcaaaaga attttttgaa tttggagatg ttgaagctaa
     3841 gcttaaagaa agtgattttg tggttgaatc ttcatacaaa acccaaagtc aatcgcattg
     3901 catgatggaa actcataggt catattcata taaagatata aatgatagaa ttgttgtagt
     3961 ttcatcatta caatcaccac acaatgtaaa gagaatactt agaaaagtat tgggtatacc
     4021 gtcttcaaaa ataagagtaa ttaaacctag aataggtggt ggatttggag gaaagaatat
     4081 agcagcaaca gatatattta ctgcatttgt tactatgaaa actggtagac cagcaaaatt
     4141 tgtatttaca agaagtgaaa cttttgtagg ttctaatagt agacatgaaa tgttttttga
     4201 tgttaaagtt ggtgcagata aagaaggaaa tttaaaagca atagagctaa agtcattaaa
     4261 caatacaggt gcttatggtg ggaatggtgt ctctgtttct tccgaatctg gacacaatac
     4321 cttaccaata tataatgata ttgatgctgt tagatttaat gctagaacag tatatactaa
     4381 taggttacca gcaggagcac ttagagggta tggtgctaca cagggaatat ttgcaataga
     4441 tagtgctatt gatgaattgg cacataagat tggaatggac ccacttgaat ttagaattaa
     4501 aaatataata gataggtata ccaatggaaa agcagctgaa gacgaaatat taagttgtga
     4561 gctgagacaa tgtatagaaa agggaaagaa attaattaat tgggatgata aatatccatg
     4621 taaagatata ggaaacaaca aagtaagagc agtaggtatg gcagtagcaa atcatggttc
     4681 tggtataccg cgtgtagata tggcaacagt cactataaga atggatgaag atggcacata
     4741 taaaatatta tctggtcttg ctgacttagg tcaaggggct gatacaattc aagctcaaat
     4801 tgctgcggaa gctttaaata ctactgtaga tagaatttct atttatacag gtgattcaga
     4861 cttatgtccc tatgatacag gagcatttgc ttcttgtacg acaacggtta caggaaatgc
     4921 aacaataaag acagcaaata aattaaaaga tatattactt tcagttgcag agtcaaagct
     4981 aggatttaaa aaagaagatt tagagttggg aaaagacagg gtttttgtta aaaacgataa
     5041 tagcaagttt gttctattag aggaattggg tagagaatct gttggaggaa taggatttgc
     5101 tagtgatgat gtaccagtag ttttggaagc aacagaatct tttggcatgt tgaaaaaaat
     5161 aaaaccattt atagcaggat ttgcagagat agaattagat aaaaatacag gtgaatataa
     5221 agtcataaat tttgcatgtg tacctgattg tggaacagtt ataaatccaa ctttagctag
     5281 aatacaagta gaaggtggtg tgatgatggg tatagggttt gcaatgtatg aagaccctat
     5341 atttgataaa gatggtagat tattaacaga tagtttccta cagtataaag ttcctacaaa
     5401 aaaagaagtt ggaaatatat tggtagattt tgctgagaat ttcgacccag gaggtcctta
     5461 tggtgctaaa tcattaggag aaatagttat acatacacca gcaccagcta tagcaaatgc
     5521 aatatttaat gctacaggag ttcgtttaag agagcttcct atgacttttg aaaaagttta
     5581 tatgggtatg aaaaataata ataaacaatt ataataaata ataagatgca taaaagcaag
     5641 taaatttaaa ttattttgta aaataaagta attaaatgga cattatatat ggtgttttgt
     5701 aaaaataccg tatataatgt ctagtagagt atattctaaa ggaggtgagt tcatgtcaaa
     5761 cagaataaaa aatatacact atggaactca gagacaacaa ttaaaaaact taactaaaaa
     5821 agaatatcaa gctctacgag agatgtgctt cttagctaag aacttatata atgttggtac
     5881 atataatgta agacagcatt atttcaatca aggagaacat cttaactatg aagctaatta
     5941 tcatgtatgc aaagaaaatg agaactataa gcttctcaat agtaatatag cgcaacaaat
     6001 attaaaagaa gtagacagtt cgtttaggtc attttttgga ttaattaaat tagccaaaga
     6061 aggaaaatat gactttagag ggataaaatc acctaaatat ttagataaag aaggattttt
     6121 tagcataata ataggtcaaa taagaatcaa agaagatggg attttaaatg tacctatgtc
     6181 tccagcattt aaaaaactat atgggaaagt aagtataaaa gttccacaaa atatactaga
     6241 gaaaaaaatt aaagaaataa gaataatacc taaacataaa gctaggttct ttgaaattca
     6301 gtattgttat gaaataccaa aatcagagga agtttcaaat caaaaaaatg cactagctat
     6361 agacttgggt ttagaaaatt tatgtacatg tgttaccaat ttaggtgata gttttatcat
     6421 agatggaaaa aggctaaaat ctgtgaatca gtgggcaaac aaacagaatg ataaaataga
     6481 agccttaaaa ttaaaaaaga atataactgc aataacaaga aaacaattta aagtttggaa
     6541 taagagaaat aatcaagtta aagactatgt aaataaaacc tgtttttata taattgatta
     6601 ttgtgtaaaa aataatattg gaaatttaat tgtaggatat aatgctaagt tagaaaaaag
     6661 tgataatatg gagagaaaaa tatatagaaa ttttataaga atcccatttg gagagataaa
     6721 aggaaaatta gaatatctgt gtaaaataaa aaatatcaat tttattaggc aagaagaaag
     6781 ctatactagt aaatcagatt tttttgctga tgattttcta cctgatatca atgtggacaa
     6841 tgttaaaaaa tatgacttta aaggggaaag aataacacgt ggacagtaca aatccagtgt
     6901 tggaatcatt ataaatgcag atgtaaatgc gagttttaat atactaaaaa aatcacaagt
     6961 agttaattta tctaatttac agaataatcc tcaaatatta aagcaaccac agagagtaag
     7021 aatatcttaa ttatatttaa aagataaact gatagcgtaa gcttacagct tagcggcgaa
     7081 ttggatccgc cttgttgttt acaacagaat ctatttactt tcgagtttat ggaatttcaa
     7141 aaatcacttt tactaaaaag gcctaactat aataattaag agattagaaa actggttata
     7201 agaggtgaaa gtgtggaact atcaaatata atagaaaaga atgaaataat aactgtagtt
     7261 ggagcaggag gaaaaacttc gtttataaac tattttgcaa atttttatag aaataaattg
     7321 aaagttcttc ttacaacaac aactaaaata tatgtaccaa acgattatga caatataatt
     7381 attacaatag atggaactgt tattccatct atttgtcatg gaataacagt ctgtggcagt
     7441 tatataaata atgaaaataa attagtaagt attgattcct ctattttgga tgaaattgta
     7501 gaccaatttg atttagtttt aatagaggga gatggctcaa agagaaaaaa acttaaaggt
     7561 tggaatgcta aagaaccagt agtatatcat agaactacaa aaaccatagg gatattagat
     7621 ataacatcat ttggaatgaa tattaatgaa gagaatatac atagagtaga aatatttaaa
     7681 aaaatagcaa atttagatac aagtagtatt aatagtagtt ctacagtaag tattgaaaac
     7741 ttaaaaaata tagtattaaa tccaaatgga ttatttaaaa attattctgg a
//
LOCUS       DAMJYR010000159         7762 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.164, whole genome shotgun sequence.
ACCESSION   DAMJYR010000159 DAMJYR010000000
VERSION     DAMJYR010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7762)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7762)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7762
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.164"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            236..613
                     /locus_tag="PC471_003360"
     CDS             236..613
                     /locus_tag="PC471_003360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HDF2790446.1"
                     /translation="MMIRKIPQAELKVMKFIWKVDVTVTSKDVIEAMEQKYGWKQTTT
                     LTLLSRLVKRGFLDAQKIDRYTHYTIRVKHKDYLSFETKDFLSNIHDDSLRSLISALH
                     DDENIGKDKLDFLESYFDNLKDE"
     gene            606..1754
                     /locus_tag="PC471_003361"
     CDS             606..1754
                     /locus_tag="PC471_003361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease"
                     /protein_id="HDF2790447.1"
                     /translation="MNNYMLYVFENLIKTTIVCSLGICLLLFLKRYLFKKFSKRFNYY
                     IWLIIVFRMLLFLFNYTIVYEVKEPKENALGNNITQIDISTDNNLMLYVAYLWLFVTI
                     VIAVYTFIKYTRFKNLVVDVSYDIEDNDINCIYKNLLKELNIKKKIELRGSDELISPA
                     GMGLFKSYIFLPDYPYSKDELTWILKHELMHFKNKDILIKFLVLSVRIIYWFNPLVYV
                     MSNKVNLDCELCCDESVLVECSLKDKKEYALALIKSIKFSKNYNSKILTTEFDKTNLE
                     KRLESIVKKKGKSGILIAVLVFMTSITSFVEVDAQVRIDEGNNGMNNNINEFLLNKTT
                     DKIINIYYKDAPNEIRKFYEDKCELEGKIPRDYDIIEMDMKDYNQLIK"
     gene            1771..2373
                     /locus_tag="PC471_003362"
     CDS             1771..2373
                     /locus_tag="PC471_003362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HDF2790448.1"
                     /translation="MLTKLRKIVAIMCCLLLTFTSVAFADTSKEKGGSKETVPEKNLD
                     SFTLSGYIYTYENAPQEIRDEHKQNCEEINIDPKPDDEIFVPESANLMNEDNSKGVYA
                     SYTVSYNIGAKTITIMSNEYLTISTTKVIRKGNSGKEVKAAQIMLTLLGYNVGIDSSF
                     GSKTYNAVVSFQKKYGLSADGIIGPATWDKLGRLTDPTLS"
     gene            complement(2466..3488)
                     /locus_tag="PC471_003363"
     CDS             complement(2466..3488)
                     /locus_tag="PC471_003363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HDF2790449.1"
                     /translation="MDYNDLLKSARENFNGTCKVCKICNGLACAGDVPGMGGKGSGSS
                     FIENRKSLEKIKINMRVIHNVSKPDTSIELFGRKMSSPIFAAPVSGTILNMGGKVSEK
                     EYIEPVVRGCSNSGIYAMVGDTNVDTFLLDNLDVLKDNRGNGIVFIKPWNNSKIIDKI
                     RLSEEAGAFAVGVDLDACGLINNQFQENPFSPKTIDEIRELVESTRLPFIIKGIMTVD
                     DALMAVESGASAIIVSNHGGRVLDYTPGTCEVLPDIAKSVKGKITILVDGGVRTGVDV
                     VKMLGLGADAVLMGRPFVTASFGGGLDGVEFFIEKVRNELCETMILTGCQNVKDIDGR
                     VIWNLL"
     gene            complement(3817..3999)
                     /locus_tag="PC471_003364"
     CDS             complement(3817..3999)
                     /locus_tag="PC471_003364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2790450.1"
                     /translation="MYKDKSDECIHLMTAYIDSISGYYSFIDTQLEDFMMKYGENIVD
                     SNLHSIMMLLCKWGLS"
     gene            4312..4731
                     /locus_tag="PC471_003365"
     CDS             4312..4731
                     /locus_tag="PC471_003365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3785 domain-containing protein"
                     /protein_id="HDF2790451.1"
                     /translation="MSLYKFIYDDKEYLLKEENCSALINDEENPVQGVSISKIIDILN
                     EAEEVDFDVEYYQEACPLCLEGVKEKKKFFPFLEYHFYIFSKDGEYVISNISVDYKGL
                     SFNKLSRANKVDNSYIVSVIICENCQDYIVQIENCIV"
     gene            4862..6703
                     /locus_tag="PC471_003366"
     CDS             4862..6703
                     /locus_tag="PC471_003366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HDF2790452.1"
                     /translation="MEEIDDTILDSMFKLTLNERLLTNNDFIENKIDVVKNLIDCYKT
                     EQNSLMLLKSNFLLALLYGIQGFSEKMKEYILISCKYIDKCLPKDYKFLARFYSQIAI
                     LSLKNEDVNKANLYMDKFNLICDENNFLIEEIIFKSQLIYIKASKCIESSVIIKEIET
                     LYIKIKEINDFNCKKIYFFIIGKIYFLFLDDALIAKVNFLKARKYADLLKDMQVSSLC
                     NIKIGECECSFENYECSMEYFNEVISNKKYRNVNIIQKYRASNNITKILIKTRNYPVA
                     INNLAKSEVYLEKIKNHSLKEVEKFDLFISLAMYYAKSNEKSFEQSICYLDRAKNILN
                     MIKNIEEYIIYDLEIIYHQAKIYYIFGYYDNALNTNKKLLEKSKNAKNYNYVKLAYKS
                     IYLCFEKIQNYEMSMKYFKMYYELKMMYIKARNRNYIDSLNYRHEYIEKEINNMRKVK
                     LDLDKKKYVDHLTSAYNRTFLNDFLEKQEVSEYCTAFMIDVDYFKEYNDTYGHYNGDI
                     ALKNITIIIKKYLKKDMLMIRYGGEEFLILSVCKDYKKSKIFGRKLCRIVKKFLNNNL
                     TVSIGIDTCKNSSIDIKEIIENADKALYKAKESGRNRCLHYHDFKNF"
     gene            6730..>7762
                     /locus_tag="PC471_003367"
     CDS             6730..>7762
                     /locus_tag="PC471_003367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HDF2790453.1"
                     /translation="MKRSLIEDLKRNINKIEYDMLTKFYLNLTDLNYILDYHKDLLNT
                     KLEDNSRVYTLYLISLINYIKKDREASYNYLIESLKYIKKLSLKDDSDIISKIYIYLT
                     LSAISIRKYDKVNNFYFSAKKIIRQNHLNELLVLLNMSLCVEISALYQTKTDVIALVE
                     EVSFFKTLKNKALVSRANYIFGRVYLYLFNNFIKSMEYLIECLKLAQENNLYSIEAFC
                     KCIISLCYLESGNNVEAIKYINNVLDVAHSNNSISVTERVSIKIDLIWAYLKEDMNKE
                     AEVILLKTIKLMKVVEGVYKDYLYSFIFLYSAEIELKKDNCSFEKVNTYLILSKNIYK
                     SFGDVFIYNS"
BASE COUNT         3170 a          810 c         1114 g         2668 t
ORIGIN      
        1 taggaatttt tattaaatag ttttaatgtg taaataacat tctacactat tgtaattaaa
       61 tatgtaaaaa gtaaaatatt taaatagata tattttaaaa tctaactttt aatattgtaa
      121 aaaaattaga aatttaacaa aaagtacaaa aattcacatt tttttcataa agtgtttgac
      181 taaaaaattc acatgttgta gaattaaggt agcaggagtt ttgagggagg tgtaaataat
      241 gataagaaaa ataccacaag cagaactaaa agtaatgaaa ttcatttgga aagtagatgt
      301 tacagtgaca tcaaaagatg ttattgaagc tatggaacaa aaatatggct ggaaacaaac
      361 caccacactg acacttttat ctaggctggt aaaaagagga ttcttagatg ctcaaaaaat
      421 agacagatat acacattaca caataagagt aaaacacaaa gattatttaa gttttgagac
      481 aaaggatttc ctaagtaata tacatgatga ctctttaaga agcttaattt cagcattaca
      541 tgatgatgaa aatataggta aagataagct agattttcta gaaagttatt ttgataattt
      601 aaaggatgaa taattatatg ctatatgttt ttgaaaatct tataaagaca actattgtat
      661 gtagtcttgg tatttgtcta ttattatttt tgaaaagata tttatttaag aagtttagta
      721 agagattcaa ttattatatt tggttgataa tagtctttag aatgttactt tttctattta
      781 attatacaat agtgtatgaa gtaaaagaac ccaaagaaaa tgcattagga aataatatta
      841 cacaaattga tatttctaca gataataatc ttatgctgta tgtagcttat ttatggttat
      901 ttgtaactat agtcattgca gtttatacat ttataaagta tacaagattt aaaaatctag
      961 ttgttgatgt atcttatgat attgaagata atgatataaa ctgtatctac aaaaacttgt
     1021 taaaagaatt aaatataaaa aagaaaattg aattaagagg ttctgatgaa ttgataagtc
     1081 cagcaggtat gggattattt aaatcttaca tattcttacc agactatcct tatagtaaag
     1141 atgaactaac ttggattttg aaacatgaat taatgcattt taaaaataaa gatattttaa
     1201 taaagttttt agttttgtct gttagaataa tatattggtt taatccactt gtatatgtaa
     1261 tgagcaataa agtaaatcta gattgtgaat tatgttgtga tgaaagtgtt ttggtagaat
     1321 gttctttaaa agataaaaaa gaatatgcac tagcacttat aaagtcaatt aagtttagta
     1381 aaaattataa tagtaagatt ttgacaaccg agtttgataa gacaaatttg gaaaagagac
     1441 ttgagagtat tgttaaaaag aaaggcaaaa gtggaatact aattgctgtt ttagtattta
     1501 tgacttctat aacatctttt gttgaagttg atgctcaagt tagaattgat gagggtaata
     1561 atggaatgaa taacaatatt aatgaatttt tattgaataa gacgactgat aaaattataa
     1621 atatttatta taaagatgca ccaaatgaaa ttaggaaatt ttatgaagat aaatgcgaat
     1681 tagaaggtaa aatacctaga gactatgaca taatagaaat ggatatgaaa gattataatc
     1741 aattaataaa ataattataa ggaggatttt atgttaacaa aattaagaaa aatagtagct
     1801 attatgtgtt gcttattgct tacttttaca agtgtggcat ttgctgacac aagtaaagag
     1861 aaaggaggaa gtaaagagac tgttccagag aaaaacctag attcttttac tctttctggg
     1921 tatatatata catatgaaaa tgcgccacaa gaaataagag atgaacataa acaaaattgt
     1981 gaagagataa atatagaccc taagccagat gatgagatat ttgttccaga atcagctaat
     2041 ctaatgaatg aggacaatag taaaggtgtt tatgcaagtt atactgtttc ttataatata
     2101 ggtgctaaaa ctataacaat tatgagtaat gagtatttga ctataagtac aacaaaagtt
     2161 atacgaaagg gtaatagcgg aaaagaagta aaagctgcac aaatcatgtt aactttactt
     2221 ggttataatg taggtataga tagttctttt ggaagtaaga catataatgc agttgtaagt
     2281 ttccaaaaaa aatacggttt gtctgcagat ggaataattg gtccagctac ttgggataaa
     2341 ttgggtagac taactgaccc tactctttca taggattaaa atctaaaaat atattaaata
     2401 gtgtctcaat atgatttttc aatttaaatg agacactatt taaatataaa tgttataaaa
     2461 ctattttaca ataaattcca tataactcta ccatctatat ccttaacatt ttgacaacca
     2521 gtcaaaatca tagtctcaca taactcattc ctaaccttct ctataaaaaa ctccacacca
     2581 tcaagaccac cgccaaaaga agcagtaaca aaaggtcttc ccatcaaaac agcatcagca
     2641 cccaatccaa gcatcttaac tacatcaaca ccagtcctaa ctccaccatc aacaagtatt
     2701 gttatctttc cctttacaga cttagctata tctggaagta cctcacaagt accaggagta
     2761 taatcaagta ctcttccacc atgatttgaa acaataatgg cactagcacc agactctaca
     2821 gccatcaaag catcatctac agtcataatt ccttttataa taaaaggtaa tctagtggat
     2881 tcaaccaatt ctctaatctc atcaattgtt ttaggagaaa aaggattttc ttgaaattga
     2941 ttattaataa gtccacaagc atcaaggtct acaccaactg caaaagcacc tgcttcttca
     3001 gacaatctaa ttttatctat tatctttgaa ttattccaag gctttataaa tacaattcca
     3061 tttcccctat tatccttcaa tacatctaaa ttatccagta aaaaagtatc tacattagta
     3121 tcgccaacca tagcataaat tccagaatta gaacaacctc taacaacagg ctctatgtat
     3181 tctttttcgg aaactttacc acccatgttc aatatagtac ctgatacagg tgcagcgaat
     3241 ataggagaac tcatttttct accaaataat tctatggatg tatctggctt tgaaacatta
     3301 tgtatgactc tcatatttat ctttattttt tctagacttt ttctattttc aatgaaagac
     3361 gaaccacttc ctttaccacc cataccagga acatctccag cacaagctaa tccattacaa
     3421 attttacata ctttacatgt tccattaaag ttctctctag ctgattttag taagtcattg
     3481 taatccatac aaaatctctc ctttttataa tattaaaatt gagtattact atattgaaaa
     3541 tatcttaatt gtaaatatag ggatgttatt atgatgagct aaactagata atacatatta
     3601 gatatttttt attgttgtag atagttaaat tatatcatat tagatagtca aattttgaaa
     3661 aaaactaact tatttaagta tacattttga aaaaattaaa aaagctatat atagattttt
     3721 aaagaaatct atatatagct tttaagttta tgtcaatctt ttttgttaat gaataaataa
     3781 tatagaatac aatatacaaa aaagtttctt gaaatgctaa gataatcccc atttacaaag
     3841 caacatcatt atactgtgta aatttgaatc aacaatgttt tcaccatatt tcatcataaa
     3901 atcctctaat tgtgtgtcaa taaatgaata atatccactt atactatcga tgtatgcagt
     3961 cattaagtgt atacattcat cacttttatc tttatacata tatatctccc cctatataca
     4021 atatataaat ttagatacca aatcgtatca tattaaatag ttaagtgtgt aacatctatg
     4081 cagttatata tttatatcat tatattaata actaaagtga gttttgacaa tagattttgg
     4141 gaaactggat gttcaaagtg caaacttgga ttttaaaatc attaattaag atagcgcata
     4201 ggaatatatg tatacataac ttaattataa agattataaa caattacttt tattaacttt
     4261 atttaaatgg tagaataact tggagattaa taattataag gaggatataa tatgagttta
     4321 tataagttta tatatgatga taaggaatat ttattaaaag aggagaactg tagtgcttta
     4381 ataaatgatg aagaaaatcc agttcaagga gtaagtattt caaaaataat tgatatactt
     4441 aatgaagcag aagaagttga ttttgatgta gaatattatc aagaagcatg tccattatgt
     4501 ttagaaggag taaaagaaaa gaaaaaattc tttccttttt tagaatatca cttttatatt
     4561 ttcagtaaag atggagaata tgtaataagc aatatatctg ttgattataa aggactatct
     4621 tttaataaac tatcaagggc aaataaagtt gataatagtt atattgtaag tgtgataatt
     4681 tgcgaaaatt gtcaggatta tatagttcag atagaaaatt gtatagttta gtgtaaagta
     4741 tgtcttaatt taagagaaat ttctaaattg agacatcaac ttttttcaaa aatagtatgg
     4801 aaaattacta aatttatgag ataatgtaag tcggaggtga gttacaatat atagtaaata
     4861 tttggaagag atagatgata caattttaga ttcaatgttt aaactaacct tgaatgaaag
     4921 attattgact aataacgatt ttattgaaaa taaaattgat gtagtaaaga atttaattga
     4981 ttgctataaa actgaacaaa atagtttaat gttattaaaa tcaaattttt tgctagcttt
     5041 attatatggt attcaagggt ttagtgaaaa aatgaaagag tatattttga tttcctgtaa
     5101 atatattgat aaatgcttac ccaaagatta taaattttta gcacgatttt attcacaaat
     5161 agctatttta agtttaaaaa atgaagatgt aaataaggca aatttatata tggataaatt
     5221 taatcttatt tgtgatgaaa ataattttct aattgaagaa attattttta aatcacaact
     5281 tatttacatt aaggcaagta agtgtataga atcttctgta ataattaaag aaatagaaac
     5341 gttgtatata aagataaaag aaattaacga ttttaattgt aaaaaaatat atttttttat
     5401 tattggtaaa atatattttt tgtttttgga tgatgctcta attgctaagg taaatttttt
     5461 aaaagcaaga aaatatgcag atttactaaa ggatatgcaa gtatcttcac tatgtaatat
     5521 aaagataggt gagtgtgaat gttcgtttga aaattatgaa tgttccatgg aatatttcaa
     5581 tgaagtaata agcaataaaa agtatagaaa tgtaaatata attcaaaaat atagagcatc
     5641 aaacaacata actaaaatat taataaagac tagaaattat cctgtcgcaa taaataattt
     5701 agcaaaatct gaagtatatc tagaaaaaat taaaaatcat agcttaaaag aagtagagaa
     5761 gtttgattta tttataagtt tagccatgta ttatgcaaaa agtaatgaaa aatcttttga
     5821 acaatcaata tgctatttag atagagctaa aaacatatta aatatgataa aaaatataga
     5881 agaatatata atatatgatt tagaaataat ttatcaccag gcaaagatat attatatctt
     5941 tggatattat gacaatgcct taaatacaaa taaaaaactc cttgaaaaat ctaaaaatgc
     6001 taaaaattat aattatgtga agttagcata caagagtata tatttgtgtt ttgaaaagat
     6061 tcaaaattat gaaatgagta tgaaatactt taaaatgtat tatgagttaa aaatgatgta
     6121 tataaaagct cgaaatagaa attatataga ttccttaaat tatagacatg agtatataga
     6181 aaaagaaata aataacatga gaaaagtaaa acttgattta gataaaaaaa agtatgttga
     6241 ccatttgaca agtgcatata atagaacttt tttgaacgat tttttagaaa aacaagaagt
     6301 tagtgaatat tgcacagcat ttatgataga tgtagattac tttaaagaat acaatgatac
     6361 ttatggacat tataatggag atattgctct taaaaatata actataataa taaaaaaata
     6421 tctaaaaaaa gacatgctta tgataaggta tggtggagaa gaatttctga tattatctgt
     6481 atgtaaagat tataaaaaaa gtaaaatatt tggaagaaaa ttatgtagga ttgtaaagaa
     6541 atttttaaat aataatttga cagttagtat tggcattgat acatgtaaaa atagtagtat
     6601 agatataaaa gaaataattg aaaatgctga taaagcttta tataaagcaa aggaaagtgg
     6661 tagaaataga tgtttgcatt atcatgattt taagaatttt taactgttga aataggattg
     6721 gtgatttact tgaaaaggag tttaatagaa gatttaaaga gaaatataaa caagatagaa
     6781 tacgatatgt tgactaaatt ctatctaaat ttgacagatt taaactacat cttggattat
     6841 cacaaagacc ttttaaatac aaaattagaa gataatagta gagtctatac tttatattta
     6901 attagtctta taaactatat aaaaaaagat agagaagcat catacaacta tttgatagag
     6961 tcattaaagt atattaaaaa attgtcatta aaagatgatt cagatataat atctaaaatt
     7021 tatatttatc ttactttatc agctatttct ataagaaaat atgataaggt aaataatttt
     7081 tatttctcag caaaaaaaat tataaggcaa aatcatttaa atgagttgtt ggtattgtta
     7141 aatatgagtt tatgtgtgga aataagtgct ttatatcaga ccaaaacgga tgtaatagct
     7201 ttagtagaag aagtgtcttt ttttaaaact ttaaaaaata aagctcttgt aagtagggca
     7261 aattatatat ttgggagagt ttatctatat ttatttaata actttataaa aagtatggaa
     7321 tacttaattg aatgtctaaa attggctcaa gaaaataatt tatactctat agaggctttt
     7381 tgtaaatgta taataagttt gtgttattta gagagtggaa ataatgtaga agctataaaa
     7441 tacataaata atgtattaga tgttgcacac agtaataact caatatcagt aactgaaaga
     7501 gtgtctataa aaatagacct tatatgggca tatttaaaag aagatatgaa taaagaagca
     7561 gaagttattt tattaaagac tataaaatta atgaaagttg ttgaaggtgt atataaggat
     7621 tatttatatt catttatatt tttatattca gctgaaattg agttaaagaa agataattgt
     7681 agttttgaaa aggtaaacac ctatttaatt ttatcaaaaa atatatataa aagttttgga
     7741 gatgttttta tatataattc ta
//
LOCUS       DAMJYR010000160         7730 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.165, whole genome shotgun sequence.
ACCESSION   DAMJYR010000160 DAMJYR010000000
VERSION     DAMJYR010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7730)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7730)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7730
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.165"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(232..2511)
                     /locus_tag="PC471_003368"
     CDS             complement(232..2511)
                     /locus_tag="PC471_003368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HDF2790454.1"
                     /translation="MLSDAKNNRYGLSIIAVISILVITISCLIYGSRLNKTLGEETNQ
                     YLSEIANQSVNVLKKQINGDIKLLESISIFIEGEESFEVDNILSILKRQAINSSFKRM
                     GVILPNGTAYTTDGYIEDFSKRDYFLKSMQGEVVITGKLKDVIEDDKNTNSNINVYSV
                     PIYKDNEVKGVIFATHSTKLYEKILSVPTFNGKGYSYITRSNGDIVLNPSSKNANSTK
                     ENLFSYIGRSDSISKSSINNMKSNIKNFKSGSLSYSMFGKGYYLSYAPIGINDWYLFS
                     EVPKTAISEKSYAIIKLTLSACVVLIIIFTALTIYILFIHKKSKEKLEEFAFKDNVTG
                     IGNSNKFNLEGGKFLSSHEKKNLVLIYFDIDKFKLVNDRFGYEEGDRVLKEIAEIIKN
                     IFKEQSVFSRISNDNFAIIFEKKNNRESIIEICELIRKKLSMIKTSLGVELNLIPSIG
                     VYFVEEGETNISTCLDKAMIAKTTVKRKYLKVYEIYAENLKETLIEERDIEQEMHDAL
                     KNEQFKVYLQPKVDLSTTKIVGAEALVRWQHPKKGLISPGVFIPIFEKNGFITELDMF
                     VFTQVCKNFKRWENENFPTFPISINLSRVHLENPGFISELERITKEYEIEPNLIEIEL
                     TESAIFDNTKILFKIMQTLKSVGFKISMDDFGSGYSSLNMLKDMPIDVLKLDRQFFIT
                     VGNAKKSQIVVSSIVQMAKQLDIKVVSEGVETVEQAEFLRFIGCDMAQGFLFARPMPI
                     EEYEKLILDRYSLNRKETN"
     gene            complement(2782..4236)
                     /locus_tag="PC471_003369"
     CDS             complement(2782..4236)
                     /locus_tag="PC471_003369"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HDF2790455.1"
                     /translation="MKLNDIIQGLDIINVKGELNIDINNVQYDSRKVTKGTLFICIKG
                     FVSDGHKYIKDAIEKGASAFLVEEDVAIKGCTFIKVKDTRKDMAKVADNFYNHPSQKF
                     NVIGVTGTNGKTSITTILNEILTLNKNKVGLIGTIKIFDGEKDIVSNSTTPESIDLQY
                     HFNNMLDNGCDYCAMEVSSHSLALNRVDETDFKLGIFTNLTPDHLDFHKDLEDYRKAK
                     EKLFFKTTMANIINIDDEGGKKIYENIKGINVPCYTYGVDTKADFMARDIKSDSDGVS
                     YRLITPSYEEVIFIPVPGMFTVYNTLAVIAACYVLGIPKPIYKEGLRLSNGVSGRFET
                     VPNDKGISVIVDYAHTPDALENVLKTTQQFAEGKIISVFGCGGDRDTEKRPLMGAIGQ
                     KYSDLCIITSDNPRTEEPEAIIKDILEGIDKKKENYHVVVDREQAIAEAISMAKKDDV
                     VIITGKGHETYQIIGKVKHHFDDKEVANECLSKM"
     gene            4400..5269
                     /locus_tag="PC471_003370"
     CDS             4400..5269
                     /locus_tag="PC471_003370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF2790456.1"
                     /translation="MDNKIGYLNDNFKIFNIRDKKDIQFEYHNHDFNKIIIFINGNVT
                     YFVDGIPYKLKPWDILFISNNEIHKPVIDSNVFYERIAIWISPEFMKKNSDASSDLEK
                     CFKIATENKCNRLRLNMHDIESIKSFIEQINISESNTEFGNEILRNTLFIQLMIFLNR
                     IFLKNENMESTEDVFYDETIKKVLNYINSNIDDKLSIDSISSEFFISKYYLMRKFKSH
                     TGTSIHNYIVQKRLILAKSLITNGHLMSEVCNKCGFNDYSSFVRAFKKNYGVSPKNYI
                     NNKNPLDITLLDE"
     gene            complement(5482..7554)
                     /locus_tag="PC471_003371"
     CDS             complement(5482..7554)
                     /locus_tag="PC471_003371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDF2790457.1"
                     /translation="MKKVFGVLQKVGKSLMLPVALLPAAGILLGVSNALAGDALLAHF
                     PALANPTFQLIISIMESSGQIIFNNLPLIFAVGVAVGLTEGEGVAALAAIVGFLILNT
                     SMGVMAGVTEDMVDNSMYANIVGIPTIQTGVFGGIIIGIVAAYLYKKFYKIELPQYLG
                     FFAGKRFVPIVTSLVAIAIGIILSFIWPPIQEGLLSFSKNVIEANQTLAAFMEGSLER
                     LLIPFGLHHVFHNPFWYQFGEYVNKAGELVMGDQKIFFAQLKDGVNFTAGTFMTGAFP
                     FMMFGLPAAALAMVHEAKPEKKKFVAGIMASAALTSFLTGITEPIEFAFLFVAPVLFA
                     IHCLFSGLSYAIMQLLGVKIGFTFSGGLIDFLLFGVLPNRTPWYLAILVGIGFAVLYY
                     SVFRFMIRKFNLKTPGREDESETNAEVIEMSKNELASEILIALGNKDNLKSLDACITR
                     LRLVVNDINKVDKEKLKSLGAAGVMVIGNNVQAIFGPKSDSIKSDIKDIIEGKTKVES
                     TVTKNVDKNKVKKNMEVANMENTLVEKIDSLTNGELLDIEEVPDSVFSSKLMGDGFAI
                     KSSDGLIYSPVDGTIGVIFPTKHAIIIKSQKSGVEILIHLGIETVNLEGNGFEVFVNV
                     GDEVKAGEKLVKMDLEYIEKNGLSTISPVVFTNLEQNQKLSIKKGVTTAKEENRVSIL
                     RDDIEKIG"
BASE COUNT         2621 a         1285 c          823 g         3001 t
ORIGIN      
        1 tactattatt actatctaaa gtattttgct ataatcccat atactcttac ctcaaaaaca
       61 ttcaaatgta aaaaatacta catattaata aactttgccc aataattcct aaaataatat
      121 cctaacaatg atttatgttt ttataatagt atatttttac ataaaaaagt gaacattgat
      181 taacaaagtt cactttttta taaattttta tgttataatt cacaatttta tttaatttgt
      241 ttcttttcta tttaaactat atctatctaa tattaatttt tcatattctt ctattggcat
      301 tggtctagca aataaaaatc cttgggccat atcacaacct ataaacctta aaaactctgc
      361 ttgctctaca gtttcaacac cttctgaaac aacttttata tcaagttgct ttgccatttg
      421 aacaatactt gaaactacaa tttgactctt ttttgcattt cccactgtta taaagaattg
      481 cctatctaat ttcaatacat caattggcat atctttaagc atatttaaag aagaataacc
      541 agaaccaaaa tcatccatag aaattttaaa tccaacagat ttcaaagttt gcattatttt
      601 aaataatatt tttgtattgt caaatattgc actttctgtc aattcaatct caattaaatt
      661 tggttcaatt tcatattctt ttgttattct ttcaagctca gaaataaagc ctggattctc
      721 aagatgtact cttgataagt ttattgatat aggaaatgtg gggaaatttt cattttccca
      781 acgtttaaaa tttttacaaa cttgggtaaa tacaaacata tccaattctg ttataaaacc
      841 atttttctca aaaataggaa taaatacacc tggacttatt agaccttttt tgggatgttg
      901 ccaacgtaca agtgcttcag cacctacaat tttagtagtt gataaatcaa ccttaggctg
      961 taaataaact ttaaattgtt cattttttaa agcatcatgc atttcttgtt caatatcacg
     1021 ttcttctatg agagtttctt ttaaattttc agcataaatc tcataaactt ttaaatattt
     1081 tctttttact gtagtttttg caatcatagc tttgtcaaga caggtagaaa tatttgtttc
     1141 tccctcttca acaaaatata cacctatgga tggaattaaa ttgagttcta ctcccaaact
     1201 tgtttttatc attgacaatt ttttacgtat aagttcacat atttctataa ttgactctct
     1261 attatttttc ttttcaaata ttatagcaaa attgtcattt gaaattcttg aaaatacaga
     1321 ttgctcttta aatatattct taattatctc tgctatttcc tttaatactc tatctccttc
     1381 ttcataccca aacctatcat ttactagctt aaacttatct atatcaaaat aaattaaaac
     1441 tagattcttt ttctcatgag aggataaaaa ttttccccct tcaagattaa atttatttga
     1501 attgccaata cctgtaacgt tatctttaaa agcaaattct tccaattttt ccttactctt
     1561 tttatgtata aatagtatat aaattgttaa tgccgtaaag attataatca agactacaca
     1621 tgctgataat gtaagtttta taatagcata tgatttctca gatatagcag ttttaggaac
     1681 ttcagaaaaa agataccaat cattgattcc tattggggca taacttaaat aatatccttt
     1741 tccaaacata ctataactta atgaacctga tttaaagttt tttatatttg acttcatatt
     1801 gttgatacta gattttgata ttgaatcact tctgccaatg taagaaaata aattttcttt
     1861 agtagagttg gcgtttttac tgcttggatt taaaacaata tcaccattac ttctagttat
     1921 ataagaatat ccttttccat taaatgtagg tacagaaagt attttttcat acaacttcgt
     1981 actatgtgtg gcaaatataa ctcctttaac ctcattatct ttataaattg gtacagaata
     2041 tacattaata ttactattgg tatttttatc atcttctatt acatctttta attttcctgt
     2101 tataaccact tctccttgca ttgattttaa gaaataatct cttttagaga agtcttctat
     2161 ataaccatct gtagtgtatg ctgtaccatt tggtaaaata actcccattc ttttaaatga
     2221 actattaata gcttgtcttt ttagtataga aaggatatta tctacttcaa aagactcttc
     2281 tccttcaata aatattgaaa ttgactctag taactttata tctccattaa tttgtttttt
     2341 taacacattg acgctttgat ttgctatttc tgaaagatat tgattcgttt cttcacctaa
     2401 tgtcttattc aaccttgaac cataaatcaa acaggaaatt gtaattacta agatacttat
     2461 gacagcaatt atggacaaac catacctatt attttttgca tcacttaaca ttatgcttcc
     2521 tgcctttctc gaatctatgt atgtataatc attatcttat gacaattatt gtaaacattt
     2581 tataataata cttatttttc catatttact gtaattatac aaaattataa actttattac
     2641 aacaataaat taattaatat agatattagt gtttacatta ttgcattatc tattttacaa
     2701 ttatttacat ataaaaaaat gtaatatcaa agtgaagctt gatattacat ttttgttctt
     2761 gtattttgtt taatttttat gctacatctt acttaaacac tcatttgcaa cttctttatc
     2821 atcaaaatga tgcttaactt tacctataat ttgataagtt tcatgtccct tacctgttat
     2881 aattacaaca tcatcctttt ttgccatact tatagcttct gctatagctt gttctctatc
     2941 aacaacaaca tggtaatttt cttttttctt gtcaattcct tcaagtatat cttttattat
     3001 tgcctctggt tcttcagttc ttggattatc agaagttata atacataaat cagaatattt
     3061 ttgtccaatt gctcccatca aaggtctctt ttcagtatct ctatctccac cacacccaaa
     3121 cacagaaatt atttttcctt ctgcaaattg ttgagttgtc tttagtacat tttcaagtgc
     3181 gtcaggagta tgagcatagt ctacaattac gcttatacct ttatcatttg gaactgtttc
     3241 aaaccttcct gatactccat tagaaagtct aaggccttct ttatatattg gtttaggtat
     3301 gccaagtaca tagcaagcag ctataactgc taaagtatta tatactgtaa acatccctgg
     3361 aactggtata aatattactt cttcatatga aggtgttatt aatctataag atactccatc
     3421 cgaatctgat tttatatctc tagccataaa atcagcctta gtatcaacac cataagtgta
     3481 acaaggtaca tttatacctt ttatattttc atatatcttt tttccacctt catcatctat
     3541 gtttattata tttgccatag tagtcttaaa aaataatttt tcttttgctt ttctataatc
     3601 ctctaaatct ttatgaaaat ctaaatggtc tggtgttaaa tttgtaaata tgcctagttt
     3661 aaagtctgtt tcatcaactc tatttaatgc caatgaatgt gatgaaactt ccattgcaca
     3721 gtagtcacat ccattatcca acatattatt aaaatgatat tgtaaatcta tactttctgg
     3781 agttgttgaa ttggaaacaa tgtctttttc tccatcaaaa atttttatag ttcctataag
     3841 tccaacttta ttcttattta aagttaatat ctcatttagt atagtagtta tactagtttt
     3901 tccattagta cctgtaactc ctataacgtt aaatttttga gatggatgat tgtaaaaatt
     3961 atcagctact tttgccatat cttttcttgt atcttttacc tttataaatg tacatccttt
     4021 tattgctaca tcttcttcaa caagaaatgc acttgctccc ttttctatgg catcttttat
     4081 atacttatga ccatctgaca caaatccttt tatacatata aataatgtac cttttgtaac
     4141 ctttcttgaa tcatattgta cattattaat atctatattt aattcaccct tgacattgat
     4201 tatatccaac ccctgtataa tatcatttaa tttcataaat gctttcctcc ctttaaactc
     4261 tttctgctaa aattacacta catataatat tatattaaga attttttact atgtgaatgg
     4321 ttaaaaatgc ttgaaacatt gcaaaaaatg ctctataatt ttctataata taactgagga
     4381 aaggtcttct ataaaaacta tggataataa aatcgggtac ttaaacgata actttaaaat
     4441 atttaatatt agagataaaa aagatataca atttgaatac cataatcatg attttaataa
     4501 gataattata tttataaatg gaaatgtaac ttattttgtt gatggtattc cctataaact
     4561 aaagccttgg gatatactct ttatcagcaa taatgaaatc cataaaccag ttatagattc
     4621 aaatgtcttt tatgagagaa ttgcaatatg gattagccca gaatttatga aaaaaaatag
     4681 tgatgctagt agtgacttag aaaaatgttt taaaatagct acagagaata aatgcaatag
     4741 attgcgttta aatatgcatg atattgaatc tattaagagt tttattgagc aaataaatat
     4801 ttccgaatcc aatactgaat ttggaaatga aatattgaga aatacattat ttatacaact
     4861 tatgatattc ttaaacagaa ttttcctaaa aaatgaaaat atggaatcaa cagaagatgt
     4921 tttttatgat gaaactatta agaaagtatt aaactatata aattcaaata tagatgataa
     4981 gctttcaata gatagcatat cgtctgaatt ttttataagt aagtattatc ttatgagaaa
     5041 atttaaatct catactggaa cgtctataca caattatata gttcaaaaaa gattaatttt
     5101 ggcaaagtca ctaataacca atggtcacct tatgagtgaa gtttgtaata aatgtggatt
     5161 caacgattat tctagttttg taagggcatt taaaaaaaac tacggtgtat ctcctaaaaa
     5221 ttatataaac aataaaaatc ctttagatat tactctttta gatgaataga taatttatgt
     5281 aaattttatt ttaataaaac ttgaagtatt agtgtattta tcttactaat acctcaagtt
     5341 ttttaatgtt ttaaaatata tcaaaatata atgatatata tttctattac ttaaaatata
     5401 tattaaataa attgcatgtt aactgaacct atttatatat ttattaaatc tcacataact
     5461 tttagttaca ctatcatata gctatcctat tttttcaata tcatctctta atatacttac
     5521 tctattctcc tcttttgctg tggttactcc ttttttaatg cttaatttct gattttgttc
     5581 taaattcgta aatactacag gacttattgt tgatagacca tttttttcaa tatattctaa
     5641 gtccatttta actaattttt ctcccgcttt tacttcatct cctacattca caaatacttc
     5701 aaatccattt ccttctaagt ttacagtttc tatccctaaa tgtataagta tttctactcc
     5761 acttttttgg gattttatta ttattgcatg cttagttgga aatataactc caatagttcc
     5821 atcaactggt gaataaatca atccatcact agatttaatt gcaaatccat cccccatcag
     5881 tttacttgaa aatacagaat ctggcacttc ttctatatct aataactctc catttgttaa
     5941 tgaatcaatc ttttctacta aagtattttc catatttgca acttccatat tttttttcac
     6001 tttattttta tcaacatttt tagtaacagt actttcaacc tttgtttttc cctctattat
     6061 atcttttata tcagacttta ttgaatctga ttttggtcca aatatagctt gaacattgtt
     6121 gccaataacc ataacacctg ctgctccaag tgatttcaat ttttctttat ctactttatt
     6181 tatatcattt acaaccaatc taagtcttgt tatacacgca tctagactct ttaagttgtc
     6241 tttatttcct aaagcaatca aaatctctga tgctaattca ttcttagaca tttcaatgac
     6301 ttcagcgtta gtttctgact catcttctct tcctggtgtc tttaaattaa attttcttat
     6361 cataaatcta aatacactat agtataatac tgcaaaacca atacctacca agatagccaa
     6421 gtaccatgga gttctattag gaagaacacc aaataataag aaatctatta atcctcctga
     6481 aaatgtgaat ccaattttaa ctcctaataa ttgcataatt gcgtatgata aacctgaaaa
     6541 taaacaatgt attgcaaata atactggtgc aacaaataaa aacgcaaact ctattggctc
     6601 ggttattcct gttaaaaaag aggttaatgc tgctgatgcc attatacctg ctacaaattt
     6661 tttcttttct ggctttgctt catgcaccat agcaagagct gctgctggaa gaccaaacat
     6721 cataaatgga aatgcacctg tcataaaagt acctgctgtg aaatttactc catctttaag
     6781 ttgagcaaag aatatctttt ggtcacccat tacaagctca ccagctttat ttacatattc
     6841 tccaaattga taccaaaatg gattatggaa aacatgatgc aaaccaaatg gtattaatag
     6901 tctttccaaa gaaccttcca taaatgctgc tagtgtttga tttgcttcta taacattttt
     6961 tgagaaacta agtaatccct cttgtattgg aggccaaata aaagacaata taattcctat
     7021 tgctatagca actaatgaag taacgattgg tacaaatctt ttacctgcaa aaaaacctag
     7081 atactgaggt aattcaattt tataaaactt tttataaaga taagcagcca ctatccctat
     7141 aataatacca ccaaataccc ctgtttgaat tgttggtatt ccaacaatat ttgcatacat
     7201 agaattgtct accatatctt ctgtaacacc tgccataaca cccatagatg tattgagaat
     7261 taaaaaacct actatggctg ccaatgctgc aacaccttct ccttcagtta aaccaacagc
     7321 taccccaact gcaaatataa gtggtaagtt attaaatata atttgacctg aactttccat
     7381 tatacttatt ataagttgaa atgtaggatt cgccaaagct ggaaaatgag ccaacaatgc
     7441 gtctcctgct aaagcattac tcacgccaag taatatacct gctgcaggaa gtaatgcaac
     7501 tggtagcatt agagattttc ctactttttg taaaacacca aatacttttt tcatttactt
     7561 ttctctcctt attattttat atttttttaa ataaaaaaga cttaagttta taaaaagtta
     7621 cattagtata cttaattcta aaattaaact tatgttaata taaacaaacc tatattaacc
     7681 taatttcaga attaaaaata aactattata atttttttat aaacataagt
//
LOCUS       DAMJYR010000161         7715 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.166, whole genome shotgun sequence.
ACCESSION   DAMJYR010000161 DAMJYR010000000
VERSION     DAMJYR010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7715)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7715)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7715
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.166"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            322..1623
                     /locus_tag="PC471_003372"
     CDS             322..1623
                     /locus_tag="PC471_003372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDF2790458.1"
                     /translation="MGKSKDVEIEKSILGTILLDNKLSYKLDELNENMFMNDICLEIF
                     KIMKELKKENIVIDVATVKSKIDRKSLAIKTSDVTNLITWGQNFGLDGHIKILKENLA
                     RRSINQNCQNLLHSLNLGENIDTCIYKFESNIKEILDKDTYENDDVNSIAGKVLDFLE
                     NKKDIGFKFGIKLLDTTIGGLFKGELTTIAAKSGVGKTALALQIMLNSFKQGKKTLFI
                     SREMTSEQVFMRNICRVTGVSTRNMKSKEIDENDWKLIVNAIGDLSENNLIYINDKID
                     TISAIRKRIRQVKPDLLIVDYVQLLTSQKSMDKREREVATFSRELKNMTLDFNIPVIQ
                     LSQLNDEMKDSRPYGDRPMRDSKAIYHDSNNVVYIHQLKGSDYEEAVRDIGESEEAVR
                     ASEYRGIKMVDLIVAKCRDGQTRHKHFCYFGDKLHFQELNY"
     gene            1699..1974
                     /locus_tag="PC471_003373"
     CDS             1699..1974
                     /locus_tag="PC471_003373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDF2790459.1"
                     /translation="MLELRKIIELLEEHKIEVSIPKELHKYSLLEYLELCQQSGGTYF
                     YIKTLEGALKEARNELMIKLFNGKNYMELARMFEMSVINVRHIVRKK"
     gene            3309..3539
                     /locus_tag="PC471_003374"
     CDS             3309..3539
                     /locus_tag="PC471_003374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HDF2790460.1"
                     /translation="MSNKKKKYANILANECVACGSCIKACPRSAISVPCGISAKINRD
                     LCVGCGICEKICPASVIEIITILKEEEGKCHE"
     gene            3532..4113
                     /locus_tag="PC471_003375"
     CDS             3532..4113
                     /locus_tag="PC471_003375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HDF2790461.1"
                     /translation="MNNKKHWYDYLWIFSSIYLILGFVNIIFAWIGLICFFVPLAISI
                     VKGNKAYCNKYCGRGQLFNLLGNKLKLSRNRDIPKFIRSKWFRYGFLTFFMVMFANML
                     FSTYLVFEGSRNFKEVVTLLWTIKMPWQWAYHGTLVSPWVVQFAFGFYSVMLTSTVLG
                     LITMILFKPRSWCVYCPMGTMTQMICKAKSKSV"
     gene            complement(4692..5369)
                     /locus_tag="PC471_003376"
     CDS             complement(4692..5369)
                     /locus_tag="PC471_003376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HDF2790462.1"
                     /translation="MISQNEIDILTHSLPFWDKLTDLQKELLISSANTSHYKKGNPVH
                     CGDSDCIGILIIKSGTVRTYILSDEGREVTLFRLDDGDVCILSASCILKTITFDVYVD
                     AETDCDIIQISSSVFAKLSTENIHAELFSYKLATERFSDVMWAMQQILFMSFDKRLAS
                     FLIDEIAKNGSSTINMTHEQIAKYMGSAREVVSRMLKYFAREGIVSLSRGGIKVLDKD
                     KLRSLTL"
     gene            complement(5558..6922)
                     /locus_tag="PC471_003377"
     CDS             complement(5558..6922)
                     /locus_tag="PC471_003377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HDF2790463.1"
                     /translation="MKESNKRIELLGSAPISKALLSMGLPTMIGMMITALYNLVDAYF
                     VGGLGTSQMGAISVAFPLGQLVVGLGLLFGNGAASYVARLLGHGDNEKAGKVVSTSIY
                     SSIIVGTLVIIVIMIFLNPILKLLGATKSIMPYAISYASIYVVSSIFNIFNVTMTNIV
                     TSEGAAKTTMCAMLLGALLNMILDPIFIYTFGLGVRGASIATAISQIASTLVYVGYIL
                     SKKSIFSFRINECCFSKEIFSEILKIGIPTLIFQLLTSLAITLTNMEATKYGDSVIAG
                     MGAVTRITSLGSLVVFGFIKGFQPIAGYNFGSKNYDRLHIAIKTSILWTTVFCVVLGL
                     ILILFPKSIISQFTSGDILLIENGSKALRANGFSFMLFGFYTVYSTLFLALGKAKEGG
                     FLGMCRQGVCFIPIILIIPVFYGLNGILYAQPIADLMAAIIAGFMSIRLHKELNLLKI
                     NGMH"
     gene            7050..7571
                     /locus_tag="PC471_003378"
     CDS             7050..7571
                     /locus_tag="PC471_003378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HDF2790464.1"
                     /translation="MATIDLIVLGIVKQQPKSAYDIQKAVEYRNISRWVKISTPSIYK
                     KVIQLEQKGYIESKTVKNGKMPEKAVYSLTELGETHFLDLMFDTSSKPINILLDFNAV
                     IVNLNSLTPEKQKLCLNNIEENVQELKKTIEENISIKTEVPDTAKAVLEQQLVLAQAL
                     ETWINSLKDNMGN"
BASE COUNT         3015 a          880 c         1328 g         2492 t
ORIGIN      
        1 gagtaatgaa ttatattgaa cgatatatca ataattggca gtaagttata tcaaataaga
       61 tattactatc gagtaatgaa ttatattgaa caatatatta ataattggca gtaagttata
      121 tcaaataaga tattgctatt atgtagtaaa ttatataaaa caatgtatca gaataaatag
      181 tatattaata atttgtagta tatcatgacg aataatacag tcataacaaa tagtgagtca
      241 taacaaataa tgtatttata attgataatg tatttaaaaa tcaaaatata aaaacttata
      301 aggaaagaga gattaattta tatgggtaaa tcaaaagatg ttgaaataga gaagtctatt
      361 ttaggcacaa ttcttttaga caataaatta agctataagc tagatgaatt aaatgaaaac
      421 atgtttatga atgatatatg tcttgagata tttaaaatta tgaaagaact taaaaaagaa
      481 aacatagtga ttgatgtggc cactgtgaaa tcaaagatag atagaaaatc tctagcaata
      541 aaaacaagtg atgtgactaa tctaattact tggggtcaga attttggtct tgatggtcat
      601 attaaaattt taaaagaaaa tttagctaga agaagcataa atcaaaactg ccaaaacctt
      661 ttacacagcc taaaccttgg agaaaatatc gatacttgta tttacaaatt tgaaagtaat
      721 ataaaggaga ttttggacaa ggatacttat gaaaatgatg atgtaaatag tatagcaggg
      781 aaggtgcttg attttttaga aaacaaaaaa gatattggat ttaaatttgg tataaaatta
      841 cttgatacca ctataggagg tctttttaaa ggagagctaa ccacaatagc agctaaatcg
      901 ggggttggga agactgcttt ggcacttcaa ataatgttaa attcatttaa gcaaggtaaa
      961 aaaactttat ttataagtag agaaatgacc agtgagcaag tatttatgag aaatatatgc
     1021 agagtaactg gtgtaagtac aaggaatatg aaatctaaag aaatagatga aaatgattgg
     1081 aaacttattg taaatgcgat aggtgattta agcgaaaata acttaattta tatcaatgat
     1141 aagatagata caattagtgc aattagaaag aggataaggc aggtaaagcc agatttatta
     1201 atagtggatt atgtgcaact ccttacatct cagaaaagca tggataagag agaaagggag
     1261 gtagctactt tttcgagaga acttaaaaat atgactttag attttaatat acctgtaatc
     1321 caattaagtc agttaaatga tgaaatgaaa gattcaagac cttatggaga tagacctatg
     1381 agggatagca aagccatcta ccacgatagc aacaatgtgg tttatataca tcaattaaag
     1441 gggagtgatt atgaagaagc agtgagagat attggagaaa gtgaggaggc agttagagca
     1501 tcagaataca ggggaattaa gatggttgat ttaatagttg cgaagtgtag agatggacag
     1561 actaggcata agcatttttg ttactttggg gataagttac attttcaaga actaaattat
     1621 taggtcaata aagtagtaat aacaagctta ttaaaagtcg ggaaatagca aatagattat
     1681 aaaatatgag gtgaaaatat gttggaatta agaaaaatta tagaattact tgaagaacac
     1741 aagatagaag taagtattcc aaaggagtta cataagtata gtttattgga ataccttgaa
     1801 ttgtgtcaac aaagtggggg aacatatttt tatattaaaa cactggaagg ggctttaaaa
     1861 gaggctagaa atgagcttat gattaaattg tttaatggga aaaattatat ggaattagcg
     1921 aggatgtttg aaatgagtgt tattaatgtc aggcatattg ttagaaaaaa atagatattt
     1981 ttggagaata caagtgtgat atacttctga aatagaagtt attataagag gagtataagt
     2041 gtgattaaca acatgatttt gtgaataaaa ttgaggatat atatgagtta aatgtgtgat
     2101 taataaggta agattgtgta taagattatg agtatttaaa aggaggggct ataattaata
     2161 gtgtagagtt gtgtgtttgc ttaagattat ataataattt aatagattaa actatactaa
     2221 taaggtaaaa tcgtgtgttt agttaaagct tattataaaa tcttgatatg tgattaatga
     2281 tgcaatatgt gaataaagtt aagattataa tagatttaac ctgtggctaa tagaataaaa
     2341 ttgtgaataa agttaagatt ataataggtt taaattgtga ctaatagagt aagagtgtaa
     2401 atagagataa acttcaatat gagcttaacc tatgattaat agagtaagaa tgtaaataga
     2461 gataaacttc aatatgagtt taacttgtgg ctaatagagt aagagtgtaa atagagataa
     2521 acttcaatat gggtttaact tgtggttaat agagtaaggt gtaactaaag ataaacttca
     2581 atatgagctt aacctatgat taatagagta agaatgtaaa tagagataaa cttcaatatg
     2641 agtttaactt gtggctaata gagtaagagt gtaactaaag ataaacttca atatgggttt
     2701 aacttgtggt taatagagta agaatgtaaa tagagataaa cttcaatatg agcttaactt
     2761 gtgattaata gagtaagagt gtaaatagag ataaacttca atatgggatt aacttgtgat
     2821 taatagagta agagtgtaac taaagataaa cttcaatatg agcttaacct atgattaata
     2881 gagtaagaat gtaaatagag ataaacttca atatgggttt aacctgtggt taatagagta
     2941 gggtgtaact aaagataaac ttcaatatga gtttaacttg tggctaatag aataagattg
     3001 tgaataaagt tatagtttta gatgggtatt aacctgttac taatagaata tgattaggtg
     3061 taaaattata gagctgcata agaaaatatt atagatatat taaatattag ataataaata
     3121 tttatataga tacacaaaag aaaaggcgat atgaagctaa acttaatgac ttcatatcgt
     3181 tttttatttg aaactccttt gtatttagag tgtgattttt aatattgagg ttttatgtga
     3241 ttaagtcaca gataaaaagt aagtatcttg atataattct tatagattaa taatataggg
     3301 aggttattat gtctaataaa aaaaagaagt atgcaaatat tttagcaaat gagtgcgttg
     3361 cttgtggtag ctgtataaaa gcatgtccaa gaagtgctat ctcagttcca tgtggcatta
     3421 gtgcaaaaat taatagagat ttatgtgttg gatgtgggat ttgtgaaaag atatgtccag
     3481 catctgtaat tgagattata acaatattaa aagaggaaga aggtaagtgt catgaataat
     3541 aaaaaacatt ggtatgacta tttatggatt ttttcttcaa tatatttgat attgggattt
     3601 gtaaatatta ttttcgcttg gattgggcta atttgtttct ttgttccact agctatttct
     3661 attgttaagg gaaataaagc ttactgtaat aagtattgtg gaagagggca actgtttaat
     3721 ttgcttggaa ataaattgaa gctgtcaaga aatagagata ttcctaaatt tattagaagt
     3781 aagtggttta gatatgggtt tttgacattt tttatggtaa tgtttgccaa tatgcttttt
     3841 tcaacttatt tagtgtttga aggaagtaga aattttaaag aagtagtgac tttgttgtgg
     3901 actataaaaa tgccttggca atgggcgtat catggaactt tagtttcacc ttgggtggta
     3961 cagtttgcat ttggttttta tagtgttatg cttacatcaa ctgttcttgg tttgattact
     4021 atgatattgt ttaaacctcg ttcatggtgt gtttattgcc ctatgggtac tatgactcag
     4081 atgatatgta aggcaaagag taagtctgta tagaaaggcc ttatgtgata tttacagttt
     4141 tgttcaagta gagatgagta gtaggtttga atatatatga caaatatatg agtatagggg
     4201 catagattta gtttgtgtaa ggtaaactga tttatgcttc ttttttatgt cttagattat
     4261 tggctaagtg gaaacaatga atttagatat taagaattat tcagttattt gaagaaaata
     4321 gaataataat taacattata ttaatggata ttcattacat ataagccagt taatagcaag
     4381 atttatttaa tatgatatag gtcaagtatt taaactaaat aaggtcatga caattaatat
     4441 aagtatagat ataaaaagat tagaagttat aacttataaa aggtattcta tataaatagt
     4501 atggggaaaa tagtcaaatt aacttattag aaacaaaaaa tatacataaa aaataaatgt
     4561 aaatatcaaa taagatacta tatcaaaata gatataagta tcttttttat tattgaaatt
     4621 atgaaaatat atttgatttt acacaaaaaa gagtgcctca tgaactaaaa agttcatttt
     4681 gagacacact tttacagtgt caaagaacga agtttatcct tatccaatac ctttatacca
     4741 ccacgagata aagacacaat accttctcta gcaaaatatt tcaacattct cgatacaact
     4801 tctctggcac tacccatata cttagcaatt tgctcatgag tcatatttat agtgctactg
     4861 ccatttttag ctatctcatc aattaaaaaa gatgccaaac gcttatcaaa actcataaaa
     4921 agaatttgtt gcatagccca cataacatca gaaaaacgtt cagtagctaa tttatatgaa
     4981 aataactctg catgaatatt ttctgtagat aattttgcaa aaacagagga actaatctgt
     5041 ataatatcgc agtccgtttc agcatctaca tagacatcaa aagttattgt ttttagaata
     5101 caggaagctg ataaaataca tacatctcca tcatcaagtc ggaacagtgt aacctctcga
     5161 ccttcatctg aaagtatgta tgtacgcact gttccagatt ttataatcaa aataccaata
     5221 cagtcactat caccacaatg tactggattc ccttttttgt aatgcgaggt atttgctgaa
     5281 gaaattaata attctttttg taagtcagta agtttatccc aaaaaggcaa gctatgtgta
     5341 agtatatcaa tttcattttg tgaaatcata caatatctcc tttcttaaat tgttataatc
     5401 ccatgataca agtgaataga taaattgaca agtagtttgt taagaggatt atttagtcag
     5461 atagtaatag tatgatttat gtagaaaaaa tatgataata tatattcttg ctattaatga
     5521 gaaagtaata tgttttgatg atagtaggtt tgtataacta atgcataccg tttattttta
     5581 aaagattaag ttccttatgt aaccttatag acataaaacc agcaattata gcagccatca
     5641 aatcagctat tggttgtgca tataagatac catttaaccc atagaataca ggaataatca
     5701 aaattatagg gataaaacag acaccttgtc tacacatacc taaaaatcca ccttcctttg
     5761 cttttcctag tgcgaggaat aatgtagaat acactgtata gaatccaaat agcataaatg
     5821 aaaacccatt tgccctaaga gctttactac cattttctat taataaaatg tcaccacttg
     5881 taaattgtga aattatagat tttgggaaga gtatcaatat aagaccaagc actacacaaa
     5941 atactgtagt ccataatatg gaagttttta ttgctatatg taatctatcg tagttctttg
     6001 aaccaaaatt ataaccagca attggttgga aaccttttat aaaaccaaat acaacaagac
     6061 tacctaaaga cgttattcgt gttacagcac ccattccagc tatcacagaa tcaccatact
     6121 ttgtagcctc catattagtt aatgtaatag caagactagt caataattga aatattaagg
     6181 taggtatgcc aatttttagg atttctgaaa aaatttcttt tgaaaaacag cattcgttaa
     6241 ttctaaaact aaatatactt ttcttgctta aaatatatcc tacataaaca agtgttgaag
     6301 caatctgtga gatagctgtt gcaatagaag caccccttac ccccagacca aaagtataaa
     6361 tgaatatcgg gtctaaaatc atattaagca aagcacctaa taacatagca cacatagttg
     6421 ttttagcagc tccttcactt gtgacaatat tagtcatagt gacattgaat atattaaaaa
     6481 tagatgacac gacataaatg ctagcataag atattgcata aggcataata ctttttgtag
     6541 ctcctaacaa ttttaaaatt ggattgagaa aaatcataat cacaataata actaatgtac
     6601 ccactataat actactgtaa atagatgtac ttaccacttt tcctgctttt tcattatcac
     6661 catggcctaa aagtctggca acatatgaag cagctccatt tccaaacaat aatcctaatc
     6721 ccacaacaag ctgacctaat ggaaatgcaa cggatatagc tcccatttga cttgttccaa
     6781 gtccacccac aaagtatgca tcaaccaaat tgtataatgc agttatcatc atacctatca
     6841 tggtaggtaa tcccatagat aacaatgctt ttgagattgg ggcagaccct aataattcaa
     6901 ttcttttgtt actttctttc atacttaaca ctcctcttct attgacatta agtctaaatt
     6961 ataatactat aaattatagt aagtatggaa aatagtacta taatttatag tatttgtcaa
     7021 tagtaagaaa actaaaggag atgattttta tggctacaat agatttaatt gtattgggaa
     7081 tagtaaagca acaacccaag agtgcttatg atattcaaaa ggcagtggaa tacaggaata
     7141 tatcacgttg ggttaaaata agtacaccat caatttataa aaaagtaatt caattagaac
     7201 aaaagggata tattgaaagt aaaactgtga agaatggaaa aatgccagaa aaagcagttt
     7261 attcattaac tgaattaggt gagacacatt ttttagattt aatgtttgat acatctagca
     7321 aaccaataaa tattctttta gattttaatg ctgttatagt aaatttaaat agccttacac
     7381 cagaaaaaca gaaattatgt ctcaataata ttgaagagaa tgttcaagag ttaaagaaaa
     7441 ctattgagga aaatataagt ataaagacag aagtaccaga cacagcaaaa gcagtgttgg
     7501 agcaacagtt ggtgctagca caagcattgg aaacctggat aaattcttta aaagataata
     7561 tgggaaatta gaattagaga agctaactta ataatcatta aacaaaaacc agcatattta
     7621 ataatgagat attagaaaaa ataaaacaaa tgatataaac tgaaaagtat atttaaaata
     7681 aagctcactt aattgaggga gaatgttaaa ctttt
//
LOCUS       DAMJYR010000162         7696 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.167, whole genome shotgun sequence.
ACCESSION   DAMJYR010000162 DAMJYR010000000
VERSION     DAMJYR010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7696)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7696)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7696
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.167"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            61..1119
                     /locus_tag="PC471_003379"
     CDS             61..1119
                     /locus_tag="PC471_003379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HDF2790465.1"
                     /translation="MNCKWISKIDERKKCHREADSSGYCIFHKENKSDEEIQLMMDTI
                     HKEEISEFNGFVFENEFNAEEILTYNYKILDFSESIFKQKANFKKYIFKKNIIFNYTE
                     FRDKVLFNGCVFLENCDFNRSIFSKNYINDRIFEKVKFKGPDLVVNKVENFPRMDGII
                     FSMCTKFVLKNVEYGKSEYEHGKINYRIARNQATKIGEYEMIGFYYYKERIYSSKIMK
                     RSNYPTFSDYLVEKFFDQIARYTTGYGEKPWNILLVIIAIISVFALLYLFVGIESSNS
                     TLVALDINNIGDYSLSEIFKMYMDLWYFSMATFSTVGYGDMVATSLIGKALAGIEVFF
                     GVTIGAIWASVIIKRMIR"
     gene            1223..2407
                     /locus_tag="PC471_003380"
     CDS             1223..2407
                     /locus_tag="PC471_003380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HDF2790466.1"
                     /translation="MKNALKKIISLAMILVFIIPNYSYADDKSVDKYSKTSILIDQET
                     GRVLYSKEPDMKVPLASLSKMMTFLLAIEAIENKEVKETDKVKIDKSIASVKGSSYKL
                     KDGEEVPLIELMRGLMIVSGNDAAIAIAKHICKTKEAFVDRMNKKAKEIGMSNTHFLN
                     PNGLPIYDLSNPKKPAKENISTAKDIAILGKYMFDHYEKQVTAITDMETYTNSDRSFE
                     KSNTNALLRIIPEVDGIKTGYTGNAGYCLSFSMIVNKNDKNEKKRRVIGVVLGTNHKN
                     KRTSASLALLKYGKENFNMKKVIDKDSFIGKKYIKGMKELEVTLKAKDELYTILKDDE
                     SIKSEVKIKNIEYPVKKGDTMGIIKYYTNSGDLLGSVDIVSNNSIDNVSLKTKLKMKF
                     AN"
     gene            2614..3327
                     /gene="rpsB"
                     /locus_tag="PC471_003381"
     CDS             2614..3327
                     /gene="rpsB"
                     /locus_tag="PC471_003381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HDF2790467.1"
                     /translation="MSVISMKQLLEAGVHFGHQTRRWNPKMAKYIFTERNGIYIIDLQ
                     KTVKKVEEAYKFTKEVAETGKPILFVGTKKQAQDAIKDEAERCGMYFVNERWLGGMLT
                     NHKTIKTRINKLRELEKMEEEGVFNVLPKKEVIKLRAEKEKLEKYLGGIKDMPELPGA
                     MFVVDPRKENIAIQEAHRLGIPVVGIVDTNCDPEQLDFAIPGNDDAIRAVKLITGAMA
                     TAVIEGRQGAEEEVAEDQE"
     gene            3406..4317
                     /locus_tag="PC471_003382"
     CDS             3406..4317
                     /locus_tag="PC471_003382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HDF2790468.1"
                     /translation="MANITAQMVKELRESTGAGMMDCKKALQEAEGNMEKAVDLLREK
                     GLSKAAKKAGRVAAEGLVAIEMNDDNTVASMVEVNSETDFVAKNEDFKVFVKDAACMA
                     LATDKEDIASLLGETHKEGITLQEVLNNRVAKIGEKLDFRRFAKVVTNGQVAGYIHGG
                     GKIGVLVEMETEARDAKVLELGKDVAMQVAAMNPKYVSRDEVDAEYIAHETEVLTQQA
                     LNEGKPANIVEKMVKGRLEKELKEVCLLEQTFVKNPDITVKQLVADVAKAVGSDIKVV
                     KVVRFEVGEGIQKREENFAEEVAKQLK"
     gene            4402..5106
                     /locus_tag="PC471_003383"
     CDS             4402..5106
                     /locus_tag="PC471_003383"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HDF2790469.1"
                     /translation="MDKPMYKRVLLKLSGEALAGERGFGINNDVVNDIAIAIKKIQEI
                     GVEVAVVVGGGNFWRGRTSEGMDRTTADYIGMLATVMNAMALQDALENIDVATRVQTA
                     IDMRQIAEPYIRRRAVRHLEKERVVIFGAGTGNPYFTTDTTAALRAAEMEAEVILLAK
                     NVDAVYDKDPKVHADAKKFTELSYMEVIQKELKVMDSTATSLCMDNKIPIKVFELTTE
                     NIIRAVKGENIGTTVK"
     gene            5132..5689
                     /gene="frr"
                     /locus_tag="PC471_003384"
     CDS             5132..5689
                     /gene="frr"
                     /locus_tag="PC471_003384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HDF2790470.1"
                     /translation="MKLEIHKQLEEKMNGTIDALKFEFGTIRAGRANAQMLDKIRVDY
                     YGTPTPINQIGAISVPEPRILMISPWDKSAMHEIEKAIANSDLGLNPSNDGEVIRLSV
                     PALTEERRKELAKKASKAAEEFKVRIRNERRDANEKIKKMEKGGELTEDELKKAQDEV
                     QKMTDKFIKEIDALLSKKEKDIMEV"
     gene            5692..5895
                     /locus_tag="PC471_003385"
     CDS             5692..5895
                     /locus_tag="PC471_003385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2790471.1"
                     /translation="MKKYYNLLGLHVDEVKQYFDEQNINYTVKSIEGKKDKEKLVVPR
                     VIKISEIGDSVELIITYFSDSLV"
     gene            5924..6658
                     /locus_tag="PC471_003386"
     CDS             5924..6658
                     /locus_tag="PC471_003386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HDF2790472.1"
                     /translation="MNNNIIYDIDLNNIPTHIAIIMDGNGRWAKARFLPRTAGHKAGV
                     ETIRDIVKECSKLGVKHLTLYAFSTENWKRPKLEVDTLMNLLSTYLRNEIAELHQNNV
                     KVTAIGDISALPKTCIRELNSAKELTKNNTGVNLNLALNYGSRADIKNALIDIVKNCE
                     SGKIDINNIDEDIIKNYLSTKSIPDPDLVIRTSGEQRLSNFLLWEVAYSEFYFTDIHW
                     PDFKKEELQKAIYVYQRRDRRFGGLK"
     gene            6671..7450
                     /locus_tag="PC471_003387"
     CDS             6671..7450
                     /locus_tag="PC471_003387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HDF2790473.1"
                     /translation="MLTRIIASLALVPLFLFVVYGGIPLYIAETAIVYIALHEFYKAF
                     KIKDVHPIFIIGYLFSIYLAVKNIFNLPLEYTYAVIFILFLASIIYMLMGKNNVIDVS
                     ITFLGVFYIGVFLDFIIITINGFEKGSIYVWLIFVISFMTDIFAYFSGYLLGKHKLIP
                     KVSPKKTIEGAIGGIIGSTLCCILFGYLFGIDLLQLAIIGSIGSVIAQLGDLFASSIK
                     RYVGIKDYGKIIPGHGGILDRFDSVILVAPFVYSAIKFFIR"
BASE COUNT         3172 a          785 c         1458 g         2281 t
ORIGIN      
        1 aataatatcc aaactaatac aaaatagtaa taattgtttt gtactggagg atatatattt
       61 atgaattgta aatggatatc taaaatagat gagagaaaaa aatgtcatag agaagcagat
      121 tcttctggat attgtatctt tcataaagaa aataagtctg atgaagaaat acagttgatg
      181 atggatacta ttcataaaga agagataagt gaatttaatg gatttgtttt tgaaaatgaa
      241 tttaatgcgg aagaaatatt aacttacaat tataaaatac tggatttttc tgaatccata
      301 tttaaacaaa aagcaaattt taaaaaatat atttttaaaa agaatattat ttttaactat
      361 acagaattta gagataaagt tttatttaat ggttgtgtat tcttagaaaa ctgtgatttt
      421 aatagaagca tatttagtaa aaactatata aatgaccgta tatttgaaaa agtgaaattt
      481 aaaggacctg atttggtagt taataaagta gaaaattttc caagaatgga tggaataatt
      541 tttagcatgt gtacaaaatt tgtgttaaag aatgttgaat atggaaaaag tgagtatgag
      601 catggcaaga taaattatag aatagctaga aatcaagcaa caaaaattgg agaatatgag
      661 atgataggat tttactatta taaggaaaga atttatagta gtaaaataat gaaacgttct
      721 aattatccta ctttcagtga ttatttagtt gaaaaatttt ttgaccaaat agcacgttac
      781 acaacaggtt atggagaaaa gccatggaat atattattag ttattatagc aataataagt
      841 gtttttgctt tattatactt atttgtaggc atagaaagtt caaatagcac acttgtagct
      901 ttagatataa ataatatagg tgattactca ctaagtgaga ttttcaaaat gtatatggat
      961 ttatggtatt ttagcatggc tacatttagt acagtaggat atggagatat ggttgctaca
     1021 agtttgattg ggaaagcatt ggcaggtata gaagtatttt ttggagttac aataggagca
     1081 atttgggcat ctgtgattat taaaagaatg ataaggtaat atttgtgtaa atatggatag
     1141 gtattaatta ctagatataa attggactaa atagggaaaa tttaaatata aatatactta
     1201 ataatccaag aaggtgaaaa atttgaaaaa tgcactaaaa aaaattatat cattagccat
     1261 gattctagtt tttataatac caaattattc atatgcagat gataaatctg tagacaagta
     1321 ttcaaaaaca tctatactta tagaccaaga aacaggtaga gttttatata gtaaagaacc
     1381 tgatatgaag gtaccattag ctagtttaag taagatgatg acttttttac ttgcaataga
     1441 agctattgaa aataaagaag taaaagaaac tgataaagta aaaatagaca aatctattgc
     1501 atcagtaaaa gggtcaagtt ataaacttaa agatggtgaa gaagtgccac ttatagagct
     1561 tatgagaggt cttatgattg tatcaggaaa tgatgcggca attgctatag caaaacatat
     1621 ttgtaaaacg aaagaagcat ttgtagatag aatgaataaa aaagcaaaag aaataggaat
     1681 gagtaataca cattttctaa atccaaatgg tcttccaata tacgatttaa gcaaccccaa
     1741 aaaaccagca aaagaaaata tctctactgc aaaagatata gctattcttg gaaaatacat
     1801 gtttgaccat tatgaaaaac aagtaacagc tataactgat atggagacat atacaaattc
     1861 agatagaagt tttgaaaaaa gcaatacaaa tgcattatta agaattatac cagaagtaga
     1921 tggtataaaa actggatata ctggcaatgc tggatattgc ttatcatttt ctatgattgt
     1981 aaataaaaat gataaaaatg agaaaaaacg tagagtaata ggggttgtat taggtactaa
     2041 ccataaaaat aaaagaacct ctgcttcatt agctctactg aaatatggaa aagaaaattt
     2101 caatatgaaa aaagtaattg ataaagatag ctttattggt aagaaatata taaaaggaat
     2161 gaaagagtta gaggttacat taaaagcaaa agatgaactt tatacaatat taaaagatga
     2221 tgaaagtata aaatcagaag ttaagattaa aaatatagag taccctgtta aaaaaggtga
     2281 tactatgggt attataaaat attatactaa ttcaggtgac ttacttggaa gtgtagatat
     2341 agtaagtaat aatagtatag ataacgtatc attaaaaaca aaattaaaaa tgaaatttgc
     2401 aaattaaatt caaaaaatgg tataaaatac acaaaaaaat ataaaaatag aaaatatgga
     2461 aaaaatattg ttatttcttc acctctgtga tatactacta gaggtgaaaa aataaaataa
     2521 atcacacaca gcattgggat ccttaaaagg tgcctattac atggtttttt agggagaaga
     2581 tcaagctgga ggtataaaac caggaggtaa gatatgtcag taatatcaat gaaacaatta
     2641 ttagaagcag gtgttcactt tggtcaccaa acaagaagat ggaaccctaa aatggctaag
     2701 tatatattca cagagagaaa tggaatatat ataatcgact tacaaaaaac agttaaaaaa
     2761 gttgaagaag cttacaagtt cactaaagaa gtagctgaaa caggaaagcc aatcttattc
     2821 gtaggaacta aaaaacaagc tcaagatgcg ataaaagatg aagctgaaag atgtggaatg
     2881 tactttgtta atgaaagatg gttaggtgga atgttaacaa accacaaaac tataaaaaca
     2941 agaataaata aattaagaga attagaaaaa atggaagaag aaggcgtatt taatgttctt
     3001 cctaaaaaag aagttataaa attaagagct gaaaaagaaa aactagaaaa atacttaggt
     3061 ggaataaaag acatgcctga attaccaggt gcaatgttcg tagttgaccc aagaaaagaa
     3121 aacatagcta ttcaagaagc tcatagatta ggtataccag tagttggtat agttgatact
     3181 aactgtgacc cagaacaatt agactttgct ataccaggaa atgatgacgc tataagagcc
     3241 gtaaaattaa taactggtgc tatggcaaca gctgtaattg aaggtagaca aggtgctgag
     3301 gaagaagtag ctgaagacca agaataataa tttgacaatt aaatggtaag ggtcgccctt
     3361 accatttata atatggaata gaataagtag gaggaaactt aaaaaatggc taatataact
     3421 gctcaaatgg taaaagagtt aagagaaagt acaggcgcag gaatgatgga ctgcaagaaa
     3481 gctttacaag aagctgaagg aaacatggaa aaagcagtag atttattaag agaaaaagga
     3541 ttatcaaaag ctgctaaaaa agctggtaga gttgctgctg aaggtctagt tgcaatagaa
     3601 atgaatgatg ataatacagt tgcttctatg gtagaagtta actcagagac agatttcgtt
     3661 gctaaaaacg aagacttcaa agtgttcgtt aaagatgctg cttgtatggc attagctact
     3721 gataaagaag atatagcttc tttattaggt gaaactcata aagagggaat aactttacaa
     3781 gaagttttaa ataatagagt tgcaaaaata ggtgagaaat tagactttag aagatttgct
     3841 aaagttgtaa ctaatggaca agttgctgga tacattcacg gtggtggaaa aataggtgtt
     3901 cttgttgaaa tggaaacaga agctagagat gctaaagttt tagaattagg taaagatgta
     3961 gctatgcaag ttgctgctat gaatcctaag tatgtttcaa gagatgaagt agatgctgaa
     4021 tacatagcac atgaaacaga agtattaact caacaagctt taaatgaagg aaaaccagct
     4081 aatatagttg aaaaaatggt taaaggaaga ttagaaaaag aattaaaaga ggtttgttta
     4141 ttagagcaaa ctttcgttaa aaatccagat ataactgtta aacaattagt tgctgatgtt
     4201 gctaaggcag taggttctga tataaaagtt gttaaagttg tgagattcga agttggtgaa
     4261 ggtatacaaa agagagaaga aaactttgca gaagaagttg ctaagcaact taagtaataa
     4321 ccaaagagag aacacgcagg tgttctcttt tttaaaaaat agaaaaaatt ggatatgaag
     4381 gattgaaata ggaggtttct gatggataaa ccaatgtaca aaagggtatt gttaaaatta
     4441 agtggagaag cattagcagg agaaagaggt tttggtataa ataatgatgt agttaatgat
     4501 attgctattg ctataaaaaa aatacaagaa ataggtgttg aagtagctgt agtagtaggt
     4561 ggaggaaact tctggagagg cagaaccagt gaaggaatgg acagaactac tgctgactat
     4621 ataggaatgt tagctacagt tatgaatgca atggcgctac aggatgcact agaaaatata
     4681 gatgttgcta caagagttca aactgctata gacatgagac aaattgcaga accatatata
     4741 agaagaagag cagttagaca cttagaaaag gaaagagttg ttatatttgg tgcaggaaca
     4801 ggaaaccctt actttacaac agatacaact gctgctttac gtgctgctga gatggaagca
     4861 gaagttatat tgttagctaa aaatgttgat gcagtatatg acaaggatcc aaaagtacat
     4921 gcagatgcta agaaatttac agaattaagt tacatggaag ttatacagaa agaattaaaa
     4981 gtaatggatt ctacagctac atctttatgt atggataata aaattccaat aaaggtattt
     5041 gagcttacaa cagagaatat aataagagca gtaaaaggcg aaaatatagg aactactgta
     5101 aaataatgaa gaatgaagga ggagtaaaat tatgaaactg gaaatacata agcaattaga
     5161 agagaaaatg aatgggacaa ttgatgcttt aaagtttgaa tttggaacta taagagcagg
     5221 aagagctaat gcacaaatgc tagacaaaat aagagttgat tattatggaa ctccaactcc
     5281 aataaatcaa ataggtgcta tatctgtacc agaaccaaga atattaatga tatctccttg
     5341 ggataaatct gctatgcatg aaatagaaaa agcgatagct aactcagact taggattaaa
     5401 tccatcaaat gatggtgagg ttataagact ttctgtacct gctttaacag aagaaagaag
     5461 aaaagaatta gctaaaaaag ctagtaaagc tgcagaagaa tttaaagtta gaataagaaa
     5521 tgaaagaaga gacgccaatg agaaaattaa aaagatggaa aaaggcggag aattaactga
     5581 agatgaatta aaaaaagcac aagatgaagt tcaaaaaatg actgataaat ttataaaaga
     5641 aatagatgca ttattatcta aaaaagaaaa ggacataatg gaggtataat attgaaaaaa
     5701 tattataacc ttttaggtct tcatgtagat gaggtaaaac aatattttga tgagcaaaat
     5761 ataaattata ccgtaaaatc tattgaaggt aaaaaggata aagaaaaact tgtagttcct
     5821 agagttataa aaatatctga gataggtgat agtgtagaac tgataatcac atacttttct
     5881 gactccttag tttaaggagt cagaacaata ttggaggcaa aatatgaata acaacattat
     5941 atatgatata gacttaaata acatacctac tcatattgct attataatgg atggaaatgg
     6001 aagatgggca aaagctagat ttcttccaag aactgcagga cataaggctg gagtagaaac
     6061 tataagagat atagtaaaag aatgctcaaa attaggtgtt aagcacttaa cattatatgc
     6121 attttcaaca gaaaactgga aaagaccgaa acttgaagtt gacacactta tgaacttatt
     6181 atctacatac ttaagaaatg aaatagctga acttcaccaa aacaatgtaa aagtaactgc
     6241 tataggagat ataagtgctt taccaaaaac ttgtataaga gaattaaact cagctaagga
     6301 actgacaaaa aacaatacag gagttaactt aaatttagca ttaaactacg gaagtagagc
     6361 agatataaaa aatgctctta tagatatagt aaaaaattgt gaaagtggta aaatagatat
     6421 aaacaatatt gatgaagata ttataaaaaa ctatttaagc acaaagtcta taccagatcc
     6481 agacttggta ataagaacaa gtggtgaaca aagattgagt aactttttac tttgggaagt
     6541 tgcttattct gagttttact ttactgatat acattggcct gattttaaaa aagaagaact
     6601 acagaaggct atatacgtat atcaaagaag ggatagaaga tttggaggac ttaagtaagg
     6661 agattatttt atgcttacaa gaattattgc ttcactagct ctggttccat tatttctatt
     6721 tgttgtatat ggaggtattc ctctatatat agctgagaca gcaatagttt atattgcatt
     6781 gcatgagttt tataaggcat ttaaaataaa agatgtacat ccaatattca ttataggata
     6841 tttattttct atttatttag ctgtaaaaaa tatttttaat ttaccattag aatatactta
     6901 tgcagtaatt tttattttat ttttagcaag tataatttat atgttgatgg gcaaaaacaa
     6961 tgttatagat gtttcgatta cgtttttagg agttttttat ataggagtat ttttagattt
     7021 tataattata actataaatg gatttgaaaa aggtagtata tatgtatggc ttatatttgt
     7081 gatatctttt atgacagata tatttgcata ttttagtggt tatttacttg gaaaacataa
     7141 gctaattcct aaagttagtc ctaaaaagac tatagaagga gctattggag gaataatagg
     7201 aagtacactt tgttgtattt tatttggata tttatttggc attgatttgc ttcaattagc
     7261 gataatagga agtataggaa gtgtaatagc tcaattagga gatttatttg catcttctat
     7321 aaagagatat gtagggataa aggattatgg aaagataata cctggtcatg ggggtatatt
     7381 agatagattt gatagtgtta tattagttgc accttttgta tatagtgcga ttaaattttt
     7441 tattagataa agattggttg gatttaaaag agcaatctat aattgctagt aatgaaaata
     7501 gctatgtaac ttttaaatgc agagatattc tgcagttatt agttacatgg ctattttcat
     7561 tgttcaaaaa tttagaagac cttggggaat tgtataagaa taggtattac tttataatag
     7621 tatactatat ttgtagtgag tatatttttt atactgtaac tatcattaaa aaatattttt
     7681 ttgtttaata atttcg
//
LOCUS       DAMJYR010000163         7641 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.168, whole genome shotgun sequence.
ACCESSION   DAMJYR010000163 DAMJYR010000000
VERSION     DAMJYR010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7641)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7641)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7641
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.168"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            89..1363
                     /locus_tag="PC471_003388"
     CDS             89..1363
                     /locus_tag="PC471_003388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HDF2790474.1"
                     /translation="MITISHITAKNMYKSLEERINKFPQGAPPSDTLYKILNVLYTEQ
                     EAKLVAQLPIKPFRVKTAAKIWSVSESEAYRVLDKLASKALILDIEDNKGKKYIMPPP
                     MAGFFEFAMMRTRHDIDQKLLAELYYQYMNVEEDFIKDLFYSTETKLGRVYVQEEVLT
                     NDNEVSILDYERATHIIDESTHIGISMCYCRHRMQHVGKACDAPMDICMTFDNVANSL
                     INNKFARRVDKIECKELLHQAYEHNLVQCGENVRKGVTFICNCCGCCCEAMVAAKRFG
                     NLHPVQTTSFIPNINHENCVKCGKCITACPIDAISKVKEDGKEYIKIDEDRCLGCGVC
                     VRNCHKNSIMLLKRDEKIITPANSVHRAVLMAIEKGQLQNLIFDNNALASHRAMGAIL
                     SAILKLEPAKKILASKQLKSVYLDKLLSMNDK"
     gene            complement(1524..4565)
                     /locus_tag="PC471_003389"
     CDS             complement(1524..4565)
                     /locus_tag="PC471_003389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp20"
                     /protein_id="HDF2790475.1"
                     /translation="MFKQRLSKLLSSTLVLSMLFTAAPNITFADNTKDNSEKYQSSDI
                     ELHDYSKNAESYTKTKALAKEKIQTLLSKYGAVSAQYALIDNGKIEISGNGGVYSKQD
                     NKNLNKDNMYSIASISKMFTTTAVMKLVDDGKLNLDTPVVKYIPEFKMADDRYKEITP
                     RMLLNHSSGLMGSSFKNTILLADNDSYGHDNFLKELQKQRLKAKPGAFSVYCNDGFTL
                     AEILVERVSGMSFTNFLDKYINNPLNLQNTKTTENSFDSSKLAKAYVPYWEDAVPQDN
                     LNAIGAGGLYSSAENLCTFAQTFMKNSNGILSPASVKAMENKEYLNGLWPEGEDSILG
                     YGLGWDCVNTYPFNQYNLKALTKGGDSLLFHSNLIVLPDENMAVAVLSSGGSSQLNEI
                     IGQEILLSALKEKGKIKEIKPDKTFSKPQQVKMPSSLKENSGLYASSNMIKVDVNDNG
                     TLTVSSPYIENGPEDKYVYIGQDRFVSEKGNSCLKFVKEKNNITYLNMSSYDDVPGLG
                     QTASLYYVAQKIDDNNISNSVKEAWKKRNGKDYYLVDEKYTSQSYMFGSVKATLALSD
                     ETPGYIVNTKIMDENNSNAFIEIPGVIGRDLSDIKLHKENGTEYLSFGTLTYVSEDSI
                     TNLPAEKSFTCELESNGYAKWYKIGDDIANKKIEVNLPQNSAFAVYDDKGVPVNYSLV
                     TKNNRVRLPKGGVIVFLGSPNARFEVTYQDEVNASALTGTDRYETSIKISQAGWENAE
                     NAVLINDSAIADALAATPFAYKKNAPILLTGSSQINEKTLAELKRLKVKNVYVVGGEA
                     SINEKSLDTIKSNNISVSRISGSDRYQTSMNIAKELNNISNISKISVVNGEKGLADAV
                     SIGAVSAQNDMPIILTNENSNITEINNLFKNKKIDKSYVIGGEYTVSKNIESKLQNPQ
                     RISGSTRNETNAKVIKEFYKDSKIDNLYVAKNGMNKQDDLIDGLSVGVLAGKTKSPVM
                     LVGNSLDYNQKELFKTMRFKSVTQIGGNGNENSFKQIKEIA"
     gene            4771..5070
                     /locus_tag="PC471_003390"
     CDS             4771..5070
                     /locus_tag="PC471_003390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HDF2790476.1"
                     /translation="MYKNINKKQLKEMMKNEKDVLLLDVRTKDEFKEYKIENAINISL
                     QELINNIDEIYDYKDKKVVVYCRSGHRSVTACNLLAEEGFEHLYNLNSGIIDYMS"
     gene            complement(5309..7537)
                     /locus_tag="PC471_003391"
     CDS             complement(5309..7537)
                     /locus_tag="PC471_003391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HDF2790477.1"
                     /translation="MRHLKKTNSLKEILNWNNIKNLRPSKGLKNILEIFKSDLKDIRG
                     NYAVLVIVLGLAFLPSLYAWFNIKASWDPYGSTANMLVAVTNEDKGNEIAGQKINVGD
                     QLIEQLKDNKSLGWQFVDEKTALEGVKKGTYYASLKIPKNFTKDLTSLITDDVKKGEI
                     IYTVNEKINAVAPKITDKGASTIQLQVNQTIVKTVSEIVLGISNGVGAGIEHSLPKLT
                     KVENSLIDLQKRFDHINKTVDLASDATSKLSDTVKDIKSDLPTIKKTLNDTKLLSSDL
                     KKFLEDTNDNLDELSPLIKSDLNLMVDLSSSASSLTLNLIDAVNSGSEDVPKLIDNLS
                     EKLSNLQSLNDTLVDFLTKLNQLTSNNRLDDVIDNLEDSSNKIDSSISTLNDIKNKVI
                     SGQQPSISALNNVLSLSNGIGRINLNILNNFDSKISKPINSIFANSIKVANDIITVLD
                     KAEAKLPKVEEILTTSLKLSGNAQESISLIRERLPLAKGMLDDLIDTLSKISNGEDMK
                     KLVSLLESDILTKSNFLKEPVEVKTEKLYPIENYGSAMTPFYSVLSTWVGILLISALL
                     STSVHGNYKPYEIYFGRGLTFLSIALVQGFIIGTGDILLLGVTAKHPILLIVLLMFTS
                     MVFNFIVYSLVSVFGNVGKAIAIVLLVVQIGSSGGTFPIQVTPTFFQRINPIMPFTYA
                     IGATREAVGGIYAPNLTKDILVLLLFMILSICLNVLLKGPINRVIKPFNDKFGDSNLT
                     GH"
BASE COUNT         2644 a         1096 c         1078 g         2823 t
ORIGIN      
        1 atttagaaag aattggtatt atataaagta agagaatata taataagaaa gaatatttga
       61 ttttattgga aaacaaaaga aaaagaaggt gattactatt tctcatataa ctgcgaagaa
      121 tatgtataaa agtttagagg aaagaataaa caagtttcca caaggtgctc caccatctga
      181 tacattatac aaaatactaa atgtactata tacagaacaa gaggcgaagt tagttgctca
      241 gcttccaata aagcctttca gagtaaaaac agcagctaaa atttggagtg taagtgaaag
      301 tgaagcatat agagtattgg ataaacttgc tagtaaggct ctgatactag atatagaaga
      361 taataaaggg aagaaatata taatgcctcc tccaatggct ggtttttttg agtttgccat
      421 gatgagaacg agacacgata tagaccaaaa acttttggca gagctttact atcaatatat
      481 gaatgtagaa gaagatttta tcaaagattt attttattca acagaaacga agcttggaag
      541 agtatacgtt caagaggaag ttttaactaa tgataatgag gttagtattt tagattatga
      601 aagggctact catataattg atgaatcaac tcatataggt ataagtatgt gttactgtag
      661 acatagaatg caacatgttg gaaaagcttg tgatgctcct atggatatat gtatgacgtt
      721 tgacaatgta gcaaattctt taataaataa taaatttgca agacgtgtgg ataaaataga
      781 atgtaaagag ctacttcatc aagcatatga acataactta gttcaatgtg gagaaaatgt
      841 taggaaaggg gttacgttta tatgtaattg ctgtggatgt tgctgtgaag caatggtagc
      901 agctaaaaga tttggaaatc ttcatccagt acagacaaca agttttatac caaatataaa
      961 ccatgaaaat tgtgtaaagt gtggtaaatg tataactgca tgtccaatag atgcgataag
     1021 caaggttaag gaagatggta aagaatatat aaaaattgat gaggatagat gtttaggttg
     1081 tggagtttgt gttagaaatt gccataaaaa tagcattatg ttattaaaga gagatgaaaa
     1141 aataataact ccagcaaatt cagtacatag agcagttctt atggcaattg aaaaagggca
     1201 gttacaaaat ttaatttttg ataataatgc tctggctagt catagagcta tgggagctat
     1261 actgtcagct atattaaagc ttgaaccagc taaaaagatt ttagccagta aacaattaaa
     1321 gtcagtatat ttagataaat tgttaagtat gaatgataaa tagaaactaa cttaaaaaag
     1381 gcaaagtttt agacttatct atatcttaat aaacttatca tttaatattt aaatttaatt
     1441 tttacacata taaaacttat aacttaaata aaaaaaggct atattcaatt aatattactt
     1501 gaatacagcc ttttttagtt atgttaagct atttctttta tttgcttaaa tgagttttca
     1561 tttccatttc caccaatttg tgttacagat ttaaatctca tagttttaaa tagttctttt
     1621 tgattataat ctagtgagtt tcctactagc attacaggag atttagtttt tccagccaaa
     1681 actccaacag ataatccgtc tattaaatca tcttgtttat tcattccatt tttagcaaca
     1741 tataaattgt ctatcttaga atccttgtaa aattctttta ttacttttgc attagtttca
     1801 tttctagtgc tacctgatat tctctgtgga ttttgaagtt tactttctat atttttagaa
     1861 actgtatatt ctccaccgat tacataagat ttatcaatct ttttgttttt aaacaagtta
     1921 tttatttcag ttatattact attttcatta gtaagtatta taggcatatc attttgagct
     1981 gatacagctc caatgcttac tgcatcggct aatccttttt cgccattaac tactgaaatc
     2041 tttgatatat tgctaatatt gttcaattct ttagcaatgt tcatagaagt ttgatatcta
     2101 tcacttcctg aaattctaga tacagagata ttgtttgatt ttatagtatc taaagatttt
     2161 tcatttatag aagcttctcc accaactaca tatacatttt taacttttaa tctctttaat
     2221 tcagccaaag ttttttcatt tatttgagag cttccagtca ataatatagg agcgtttttc
     2281 ttgtatgcaa atggtgttgc agccaaagca tctgcaattg ctgaatcgtt tattaaaact
     2341 gcattttcag cattttccca acctgcttga cttatcttaa tagaagtttc atatctatca
     2401 gttcctgtaa gtgcacttgc atttacttca tcttgatatg taacctcaaa tctagcattt
     2461 ggacttccta agaaaacaat tacgcctcct tttggcaatc taactctatt gttctttgta
     2521 actaaagaat agtttacagg aacaccttta tcatcataaa cagcaaaagc tgaattttga
     2581 ggaagattaa cttctatttt tttatttgcg atatcgtcac caatcttata ccattttgca
     2641 taaccattgg attctagttc acaagtaaaa gatttttctg ctggcaaatt agttatagaa
     2701 tcttcactaa catacgttag tgttccaaaa gataaatact ctgtaccatt ttctttatga
     2761 agtttaatgt cggataaatc acgacctatt acaccaggta tttctataaa agcatttgag
     2821 ttattctcat ccattatttt agtgttaact atataacctg gagtttcatc agaaagagca
     2881 agagttgcct tcacagaacc aaacatatag ctttgagaag tatatttttc atctactaga
     2941 tagtaatctt taccatttct ttttttccat gcttctttta cactatttga tatattgttg
     3001 tcatctatct tttgagctac atagtataaa cttgctgtct gacctaatcc agggacatca
     3061 tcataagagc tcatatttaa atatgtgata ttgttttttt cttttacaaa ttttagacag
     3121 ctgttacctt tttcagatac aaatctatct tgacctatgt atacgtattt atcttcaggt
     3181 ccattttcta tataaggaga agatactgtt aaagtaccat tatcattaac atctactttt
     3241 atcatatttg aagaagcata aagaccacta ttttctttta gagagctagg cattttaact
     3301 tgttgtggtt tactaaatgt tttatcaggt tttatttctt taattttacc tttttctttt
     3361 aatgcagata gtaagatttc ttgtccaatt atttcattaa gttgactact tccaccagaa
     3421 gaaagaactg caactgccat attttcatct ggaagaacta taagattact gtggaataat
     3481 aaagagtctc caccttttgt aagagctttt aaattatact gattaaatgg ataagtgttg
     3541 acacaatccc accctaaacc atatccaagt atagagtctt ctccttcagg ccatagacca
     3601 tttaaatatt ctttgttttc catagctttt actgaagcag gagaaagaat accatttgaa
     3661 tttttcataa atgtttgtgc aaatgtacat agattttctg cacttgaata taaaccacct
     3721 gcacctatag cattaaggtt atcttgcgga actgcatctt cccaataagg cacgtatgct
     3781 tttgctagtt ttgaactatc aaaactattt tctgtagttt ttgtattttg taagttaagt
     3841 ggattattta tgtatttatc aagaaaatta gtaaatgaca taccagatac tctttctact
     3901 aaaatttcag ctaaagtaaa tccatcatta caataaacgg aaaaagctcc tggtttagct
     3961 tttaatcttt gtttttgtaa ttcttttaag aaattatcat gaccataaga atcattgtct
     4021 gctaataaaa tagtgttttt aaaactactt cccatcaacc ctgatgaatg atttaataac
     4081 attcttggag tgatttcctt gtatctatca tcagccattt taaattcagg tatatacttt
     4141 acaacaggag tatctaaatt taattttcca tcatcaacca acttcataac agctgttgta
     4201 gtaaacattt tactgataga tgcgatacta tacatattat ccttatttaa gtttttgtta
     4261 tcctgtttgc tgtaaactcc accattacca gaaatctcaa ttttgccatt atcaatcaaa
     4321 gcatattgag cacttacagc accatacttt gatagcaaag tttggatttt ctctttagca
     4381 agagctttag tttttgtgta agattccgcg tttttactat aatcatgtaa ttctatatca
     4441 gaactttgat atttttcgga attatctttt gtattatcag caaaagtaat atttggagca
     4501 gcagtaaata acatactcaa tactaatgta ctagataata atttagagag cctttgctta
     4561 aacatattac attacctctt tcccttttta ataatttata attagcataa cattcttaat
     4621 tattttaatc aacagattat tatcaataaa aaaagctttt ataagtatag ctttttatat
     4681 aacagagaaa aatataaaaa taatatttgt tgttgtataa agaataaagg gtataggtaa
     4741 tatataaata aaatggagga attttagaca atgtataaga atataaataa aaagcaactt
     4801 aaagaaatga tgaaaaatga aaaggatgtt ttattattag atgtaagaac taaagatgaa
     4861 tttaaggaat ataaaataga aaatgctata aatatatctt tacaagagtt aatcaataat
     4921 atagatgaaa tttacgatta taaagataag aaagtagtag tttattgcag aagtggtcat
     4981 agaagtgtta ctgcatgtaa tttgttagct gaagaaggtt ttgagcattt atataattta
     5041 aattcaggaa taattgatta tatgagttaa ataatgtaag ttatacatta aatactatgt
     5101 atagctctgc ttattaagta ggaatatatc taaatattta taagataaat ttaggaaaga
     5161 acgaattttt tcgttttttt ctatgacagt ttttgtagta aatattagtt tttaagttgg
     5221 aaaaaggtag tagcttttta gctactacct tttaatatga acttagaata ttattaaaaa
     5281 aaatatattt aaagacagaa ttaatttttt aatgaccagt taaattacta tcgccgaatt
     5341 tatcattaaa tggttttata actctattta taggtccttt tagaagtaca tttaaacaaa
     5401 tagacagaat cataaatagt aatagaacta gtatatcttt agttagattt ggagcatata
     5461 taccaccaac tgcttccctt gtagcaccta ttgcataggt aaatggcatt atagggttta
     5521 ttctttggaa gaatgtaggt gtaacctgta tagggaatgt accaccagaa gaaccaatct
     5581 gaacgactaa tagtactatt gcaattgctt ttcctacatt tccaaataca gaaactaaag
     5641 aatacactat aaagttaaat accatactag taaacataag cagaactatc aataaaattg
     5701 gatgttttgc tgttactcct aacaataata tatctccagt tcctattata aaaccttgga
     5761 caagagctat acttaaaaaa gtaaggcctc ttccaaagta aatttcataa ggtttatagt
     5821 ttccatgtac agatgtagac aatagtgctg aaataagtaa tataccaacc catgtagaaa
     5881 gaacactata aaatggtgtc attgcagaac cataattttc aattggatat aatttctctg
     5941 ttttcacctc aacaggttct tttaagaaat tagactttgt caatatatca ctttcaagca
     6001 agctgactaa ttttttcata tcttcaccat tactaatttt gcttaaagta tcaatcaaat
     6061 catccaacat tccttttgct aaaggaagtc tttctcttat caatgatata ctttcctgtg
     6121 catttcctga aagttttaga gaagtagtaa gtatttcttc aacctttgga agttttgctt
     6181 ctgccttatc taaaacagta attatatcat ttgctacttt tatactgttt gcaaatatac
     6241 tatttatagg ttttgatatt tttgagtcaa aattatttaa aatatttaaa ttaattcttc
     6301 caataccatt tgatagactt aaaacattat tcaaagcact tatagatggt tgttgtccag
     6361 atataacttt gtttttaata tcatttaatg ttgatataga tgaatctatt ttattgctag
     6421 agtcttcaag attatctatt acatcatcca atctattatt tgatgtaagt tggtttaatt
     6481 tagttaaaaa gtccaccaaa gtatcgttta aagattgcaa attagaaagt ttttctgata
     6541 aattatctat caacttaggt acatcttcac tgccactatt tactgcatca attaaattta
     6601 atgtaagaga agaagcagaa gatgaaaggt caaccattaa atttaagtca gacttaataa
     6661 gaggagaaag ttcgtctaaa ttatcatttg tatcctctaa gaattttttt aagtcacttg
     6721 ataatagttt tgtatcattc agagtctttt taattgtagg taaatcagat ttaatatctt
     6781 taactgtatc agataatttt gaagtagcat cagaagctaa atcaacagtc ttatttatat
     6841 ggtcaaatct cttttgaagg tcaataaggg aattttcaac ttttgtcaat tttggaagac
     6901 tgtgttcaat tcctgcacca actccgtttg atataccaag aacgatttca ctaactgttt
     6961 ttactatagt ctgattaacc tgtagttgta ttgtagaagc acctttatct gtaatctttg
     7021 gtgcaactgc atttattttt tcattaacag tatagataat ttcgcctttt ttaacatcat
     7081 ctgtaattaa agatgtcaaa tcttttgtaa aattttttgg aatcttaagt gatgcataat
     7141 aagtaccttt tttaacacct tcaagagcag ttttttcatc tacaaactgc caacctaaac
     7201 tcttgttatc tttgagttgt tcaattaatt ggtctcctac attaatcttt tgtcctgcta
     7261 tttcatttcc tttatcttca tttgttactg ctacaagcat attagcagtg cttccataag
     7321 ggtcccaaga tgcctttata ttgaaccaag catacagaga tggtaaaaaa gcaagaccta
     7381 aaactattac aagtacggca taattgccac gtatatcttt taggtcactt ttaaaaattt
     7441 ctaatatatt ttttaaaccc tttgaaggtc ttaaattctt gatattattc cagtttaaaa
     7501 tttcttttaa actattagtt tttttcaaat gtctcacctc ctaaaatatt ttgataaaga
     7561 aaaaagttag atttataatc aattttaaat tatattactt tttacacaaa tagtaaagtt
     7621 aaaattttgt cttaatttta a
//
LOCUS       DAMJYR010000164         7599 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.169, whole genome shotgun sequence.
ACCESSION   DAMJYR010000164 DAMJYR010000000
VERSION     DAMJYR010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7599)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7599)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7599
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.169"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     regulatory      124..228
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            438..2912
                     /gene="gcvPA"
                     /locus_tag="PC471_003392"
     CDS             438..2912
                     /gene="gcvPA"
                     /locus_tag="PC471_003392"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HDF2790478.1"
                     /translation="MNELKRVSLYNIHKELGAKLVEFAGWEMPLEYEGINKEHEKVRK
                     SAGIFDVSHMGEVQIKGAESEKFIQNLVTNDISALKINDIIYTPMCYENGGVVDDLLI
                     YKFGEEDYLLVINAGNIDKDVAWIIKQSEGYNVDIKNISSEVSQLAIQGPKAEEILQK
                     ITDIDLNSIKFYKSIPSTKVCGCPCLVSRTGYTGEDGFEIYCKNKYVEIIWNEVLKVG
                     GEDICPAGLGCRDTLRFEAALPLYGHEINEHISPIEGGLSIFVKTNKESFIGKSILSK
                     EKESGAKRKLVGFEMQGKGMPRNGYDIRIGDKTVGFVTTGCASPTTGKILGMGIIDSE
                     YAKVGNEIGIAIRKKVVPAVIVKKPFYKKQYKKDNIILNKENKFSYIPATSEDKSKML
                     KVVGLNSVDELFSDIPEEVKLKRDLNLEIGKSELEVSKIVKRLSEENLSLEDLTCFLG
                     AGAYDHYIPSIIKHITSRSEFYTAYTPYQAEISQGTLQVVFEFQSMIAEITGMEIANA
                     SMYDGATAAIEACIMAMNQTRKSKIVVSKTIHPETLSVLRTYLQYKDCEIVEIDFCNE
                     YGTTDIEKLKASVDKDTACVLIQTPNFFGIIEEMEEIEKITHENKAMLIMSVDPISLG
                     VLKTPGEIGADIVVGEAQSLGNPLNFGGPYVGFLASKSKYTRKMPGRIVGQSLDVEGK
                     IAYVLTLQTREQHVRREKATSNICSNQALNALVASIYMATMGKEGFKEVGMQSMKKAH
                     YTYNKLVQTGKYKPIFKGKFFKEFAVQGNLNIETINDKLLEENILGGYNLEYNYPELK
                     NSTLLCVTEKRSKEEIDKLVGIMEGL"
     gene            2912..4369
                     /gene="gcvPB"
                     /locus_tag="PC471_003393"
     CDS             2912..4369
                     /gene="gcvPB"
                     /locus_tag="PC471_003393"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HDF2790479.1"
                     /translation="MKEYNSLLIDISKKGRKAYSLPKLDIDDIKIENMIDQDMARQSE
                     LNLPEVGELELVRHYTLLSNKNFGVDTGFYPLGSCTMKYNPKINEDMAALPNFTGMHP
                     YQSSDTAQGSLSLMYDLSRRLAEITGMDEVTLQPSAGSHGEFTGLMIIKAYHENRGDH
                     KRTKVIIPDSAHGTNPASAAMANFDVIQIASDKNGAVDINALKEVLNDEVAALMLTNP
                     STLGLFEKNIKEIATLVHEAGGLLYYDGANMNAIMGITRPGDMGFDVVHLNLHKTFST
                     PHGGGGPGAGPVGVKKELVPFLPIPVIERKEDKYVLNYDREKSIGKIKNFYGNFGVLV
                     RAYTYILTMGRDGLKEASEMAVLNANYIKESIKDDYILPIDTLCKHEFVLGGLPRGEA
                     NIKTIDIAKRLLDYGYHPPTMYFPLIINEALMIEPTESESIETLDSFIEAMKSVAKEA
                     KEEPELLKTAPHNTLVKRVDDARAVKKPILTWSQR"
     gene            4792..7149
                     /locus_tag="PC471_003394"
     CDS             4792..7149
                     /locus_tag="PC471_003394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HDF2790480.1"
                     /translation="MDKVNLERYSCDINVGLNKNQLQSRIDDNLINAFDNGKTKTYKQ
                     IFKDNIFTLFNLINIVLVCLLVSVGSFKNTLFIFIAVINTIIGVIQEIRAKRLLDNLS
                     VIVSNKVSVIREKEKKEIDVQELVLDDIMILKTGNQICADSKVLNGKLEVNESLVTGE
                     SDIIIKNKGDFLYSGSFVVSGEAFVRVENIGKDNYANKIANDAKISKKHNSQLRNSLN
                     AILKIVGIIIIPLGIILFAKQHFGNGDSIATSVVGTVAAVNGMIPEGLTLLTSIALAV
                     GTIKLASHKTLVQELYCVETLARVDTLCLDKTGTITEGKMQVDDIVMLEEVDINEIMG
                     NICNSLKDDNATLNAIRDKYNVLDTYKAKNLIPFSSERKYSGVTFENKGTYLLGAFEF
                     IFKEKNKKLRIEVERYSKKGNRVIVLAHSDSYMEDNNIPEDIKPLAFILILDKIRDEA
                     KETLEFFYNEGVDIKIISGDNPITVSEVARKAGLKTASNFIDATTLKNDSDIYKAVDK
                     YSVFGRVSPQQKKQMILALKKQNHTVAMTGDGVNDVLALKEADCSIAMASGSDVTKNV
                     SNLVLLDSNFASMPKVVMEGRKVINNIQRASSLFLVKTIFSFFLSLLTLFFFSRQYPF
                     VPIQLSLIGAVTVGIPSFFLALEANKSRISGNFLLNILKNALPGALCVTVNVIIVYNI
                     VEYLGYSSSVFSTMCAILTGVCGLLILRQQCLPFNKERLFLIVSMTIAFVIGILFLGG
                     LFSFVALSRELILITMALAILMPIMIKVMLFVLDEILETIVPDLN"
     gene            complement(7235..7552)
                     /locus_tag="PC471_003395"
     CDS             complement(7235..7552)
                     /locus_tag="PC471_003395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HDF2790481.1"
                     /translation="MSEFKNVTAVKKANVYFDGKVSSRVIILPNGERKTLGLMLPGEY
                     TFSTREEEIMEMLAGSMDVKLPGSNEFVTYKEGQKFNVPSDSSFDLKVNEVVDYCCSY
                     IAD"
BASE COUNT         2984 a          840 c         1350 g         2425 t
ORIGIN      
        1 aaaagagaaa taattgataa taaaaactta acaaaaaaat taatacataa aaaaaaataa
       61 atataatgtg ttgacattta attgaaatat tcatacaata aaattgaaaa taaatatttc
      121 caaatgagat tagcgagaga gtcctttata cgaaaaggcg ccgaagaaga aatttaagaa
      181 atgccaattt ttagagaagc tttcaggcaa aaatatcgct aattgatggg actctggaga
      241 gacttaagtt aagatactat aagtcaccga agaagcaaaa taatattaaa ttattaaatc
      301 tttcaggtat acaaacaggg atatataggc taggattatt ttccatgctg atatatcttt
      361 tttttatgaa aaagtacagg aaataatatc aaagttactt aaatacagta ggtctaattt
      421 tctagggagg gttatatatg aatgaattga aaagagtatc tttgtacaat atacacaaag
      481 agcttggagc aaaacttgtt gaatttgctg gttgggaaat gcctttagaa tatgagggta
      541 taaataaaga gcatgagaag gtcagaaaaa gtgcaggtat atttgatgta tctcatatgg
      601 gagaggtaca gataaaggga gcagagtcag aaaaatttat acaaaaccta gttacaaatg
      661 atataagcgc attaaagata aatgatatta tatatacacc tatgtgttat gaaaatggtg
      721 gagtagttga tgatttatta atatataaat ttggtgaaga agattattta ttagtaataa
      781 atgcaggtaa tatagataaa gatgtagcat ggataataaa acaaagtgaa ggatacaatg
      841 tagatataaa aaatatatct agtgaagtga gtcaattagc aattcaagga cctaaagcag
      901 aagaaattct tcaaaaaatc acagatatag acttaaactc aataaaattt tataagtcaa
      961 ttccatcaac aaaagtatgt ggttgtcctt gtttagtttc aagaacagga tatactgggg
     1021 aagatgggtt tgagatatac tgtaaaaata aatatgtaga aataatttgg aatgaagttc
     1081 taaaggttgg aggagaagat atatgtccag ctggtctagg ttgtagggat actttgagat
     1141 ttgaagcagc tctacctcta tatggacatg aaataaatga acacatatct ccaattgaag
     1201 gtggtttatc tatctttgtg aaaacaaata aagaatcatt tattggcaaa tcaattttat
     1261 caaaagaaaa agaaagtgga gctaaaagaa agttagtagg atttgaaatg caaggtaaag
     1321 gaatgcctag aaatggatat gatataagga ttggtgataa aacagttgga tttgtaacaa
     1381 caggatgtgc atctccaacg acaggaaaaa ttttaggtat gggaattata gattctgaat
     1441 atgcaaaagt aggaaatgaa ataggtatag caatcagaaa aaaagttgtt cctgctgtga
     1501 tagttaagaa acctttttat aaaaaacaat ataaaaaaga taatattata ttaaacaaag
     1561 aaaataaatt ttcatatata cctgctacaa gtgaagataa atcaaaaatg ttaaaagtag
     1621 ttggattaaa ttcagttgat gaattatttt cagatatacc agaagaagta aaattaaaaa
     1681 gagatttaaa tttggaaatt ggaaaatctg aattagaggt aagcaaaata gtaaaaagat
     1741 tgtcagaaga aaatttaagt ttagaagact tgacttgttt tcttggtgct ggtgcatatg
     1801 accattatat accatcaatt ataaaacata taacttcaag atctgaattt tatacagcat
     1861 ataccccata tcaagctgaa ataagtcagg gaactttaca agtagttttt gaatttcaat
     1921 ctatgattgc agaaattaca ggtatggaaa ttgcaaatgc atcaatgtat gatggagcaa
     1981 ctgctgcaat agaagcatgt ataatggcaa tgaatcagac tagaaaaagt aaaatagtag
     2041 tgtcaaaaac tattcatcct gaaactctgt cagtattaag aacttactta caatataaag
     2101 attgtgagat agtagaaatt gacttttgta atgaatatgg gactacagat atagaaaaat
     2161 taaaagcttc tgtagacaaa gatacagcat gtgttcttat acaaactcct aatttctttg
     2221 gaattattga agaaatggaa gaaatagaaa agataactca tgaaaataag gctatgttaa
     2281 ttatgagtgt agacccaata tcattaggag ttttaaaaac accaggtgag ataggtgcag
     2341 atattgttgt tggagaagct caatcacttg gaaaccctct taattttgga ggtccttatg
     2401 taggattttt agctagcaaa tctaaatata ctagaaaaat gccaggaaga atagttgggc
     2461 aaagtttaga tgtagaaggt aaaattgcat atgtattaac tttacaaact agagaacagc
     2521 atgtaagaag agaaaaagca acttctaata tatgttcaaa tcaagcttta aatgctcttg
     2581 tagcttcaat atatatggct actatgggta aagaaggatt taaagaggtt ggtatgcagt
     2641 ctatgaaaaa agcacattat acctacaata aacttgtaca aactggaaaa tataagccaa
     2701 tatttaaagg taagtttttc aaagaatttg ctgtacaagg aaatcttaat atagaaacta
     2761 taaatgataa actcttagaa gaaaatatat taggaggtta taatttagaa tacaattatc
     2821 cagaattaaa aaattcaact ttgctttgtg tgactgagaa aagaagcaaa gaggaaatag
     2881 ataagttagt tggaataatg gagggattat aatgaaagaa tacaatagtt tgttgataga
     2941 tatatctaaa aaaggtagga aagcatactc acttccaaag ttagatatag atgatataaa
     3001 aatagaaaat atgattgacc aagatatggc aagacaaagt gagttgaatt tgccagaagt
     3061 tggtgaatta gaattagtta gacattatac attattatca aataaaaact ttggagttga
     3121 tacaggtttt tatcctttgg gttcttgtac tatgaaatat aatcctaaaa taaatgaaga
     3181 tatggcagca cttccaaact ttactggaat gcatccatac caatcttcgg atacagctca
     3241 aggttctctt tctttgatgt atgatttatc aagaagactt gcagaaatta caggtatgga
     3301 tgaagttaca ctacaacctt cagcaggttc tcatggtgag tttacagggc ttatgattat
     3361 aaaagcatat catgaaaata gaggtgacca taagagaacc aaagtgataa ttccagattc
     3421 tgcacatgga acaaatccag caagtgcagc tatggcaaac tttgatgtga ttcaaatagc
     3481 atctgataaa aatggagctg tagacataaa tgcattaaaa gaggttttga atgatgaagt
     3541 agcagctctt atgcttacta atccaagtac tcttggttta tttgaaaaaa atataaaaga
     3601 aatagctact cttgtacatg aagcaggagg acttttgtac tatgatggtg caaacatgaa
     3661 cgctattatg gggataacaa gacctggaga tatggggttt gatgtggttc atcttaatct
     3721 tcataagact ttttcaactc ctcatggagg tggaggtcca ggagctggtc ctgttggagt
     3781 taaaaaagaa ttagtacctt ttttaccaat accagtaatt gaaagaaaag aagataagta
     3841 tgtactaaat tatgatagag aaaaatctat tgggaaaatt aaaaatttct atggtaattt
     3901 tggagttctt gtaagagctt acacatatat actaactatg ggaagggatg gacttaaaga
     3961 ggctagtgaa atggcagttt taaatgctaa ttatataaag gaaagtataa aagatgatta
     4021 tattttgcca atagatacct tgtgtaagca tgagtttgta ttaggtggat tgccaagagg
     4081 agaggccaat ataaaaacta tcgatattgc aaaaagacta ctagattatg gataccatcc
     4141 tccaacaatg tactttcctc ttattataaa tgaggcactt atgatagaac ctacagaaag
     4201 tgagagtata gagacattag atagttttat agaagctatg aaaagtgttg ctaaagaggc
     4261 aaaggaagaa ccagaactat tgaaaactgc tccacataat actttggtca aaagagtaga
     4321 tgatgcaagg gcagtaaaga aacctatact aacatggtca caaagataaa tagttaaaaa
     4381 gtagagattt aaagtataaa gagaattaaa tttgtaagaa aaagctatct aacttaatct
     4441 agtttagata gcttttttgt atattagtat gtatggtatt ttctatataa agttttaaat
     4501 tatattagtg cttatataga atattgatat gatgattatg tatagatatt aataagaatt
     4561 gttgaattta tcttgcctta actttattta ctcttttata ttaagaaaaa cttaactaaa
     4621 atatagcgaa attcataaaa atttaggaaa tcttaagaat tataatattt aaatctttag
     4681 atttatattt tattatataa actatagaat aataaaataa aaaaattgag aattttataa
     4741 tatatttgta gatttgagta taaaaaagtg ctaagttgaa aggagtaaaa tatggataag
     4801 gttaatttag aaaggtatag ttgtgatata aatgttggtt taaataaaaa tcaacttcaa
     4861 agtagaatag atgataattt aattaatgca tttgataatg gaaaaaccaa aacatataaa
     4921 caaattttta aagacaatat atttacactt tttaatctca taaatattgt tttagtctgt
     4981 ttattagttt cagttggttc ttttaaaaat accttattta tatttattgc tgtaattaat
     5041 actattatag gagtaataca agagataaga gctaaaagat tattggacaa tctatctgtt
     5101 atagtttcaa ataaggtatc tgtcataaga gaaaaagaga aaaaagagat tgatgtacaa
     5161 gaattagtat tagatgatat tatgatttta aaaacaggta atcaaatttg tgcagattca
     5221 aaagttttaa atggaaaatt agaggtaaat gagtctttgg ttacaggtga atctgatatt
     5281 attataaaaa ataaaggcga ttttttatat tctggaagtt ttgtagtttc tggagaagct
     5341 tttgtaagag ttgaaaatat aggaaaagat aactatgcaa ataagatagc taatgatgca
     5401 aagatttcta aaaaacataa ttctcaactt agaaattcat taaatgcaat attaaaaatt
     5461 gtaggtataa taataattcc tttaggaatt atattgtttg caaaacaaca ttttggaaat
     5521 ggagattcta tagctacatc tgtagtaggt acagtggcag ctgtaaatgg tatgatacca
     5581 gagggattaa cattacttac gagcattgct ctagctgtag gaactataaa acttgctagt
     5641 cataaaactt tagtgcaaga actgtactgt gtagagactc ttgctagagt tgatacacta
     5701 tgtcttgata agacaggtac aattacagaa ggtaaaatgc aagttgatga tatagtaatg
     5761 ttggaagaag tggatattaa tgaaataatg gggaatatat gtaattctct caaagatgat
     5821 aatgcaacac taaatgcaat aagagataag tataatgttt tagatacgta taaagctaag
     5881 aatttaatac cattttcttc agaaagaaaa tacagtgggg ttacctttga aaataaaggt
     5941 acatatttat taggtgcatt tgaatttata tttaaggaaa aaaataaaaa acttaggata
     6001 gaagttgaaa gatattctaa aaaaggaaac agagtaatag ttttagctca tagtgattct
     6061 tatatggaag ataataatat tcctgaagat ataaagccac tagcttttat attgatattg
     6121 gataaaatta gagatgaagc taaggaaact ttagaatttt tttacaatga aggtgtagat
     6181 ataaaaatta tatctggtga caatcctata acagtaagtg aggtagctag aaaagcaggt
     6241 cttaaaacag ctagtaattt tattgatgca acaacactta aaaatgatag tgatatatac
     6301 aaagcagttg ataaatacag tgtttttgga agggtaagcc cacaacagaa aaaacaaatg
     6361 attttagcat taaaaaaaca aaatcataca gtagctatga ctggagatgg tgtaaatgat
     6421 gtgctagcac ttaaagaagc ggattgtagc attgccatgg catctggaag tgatgttact
     6481 aagaatgtgt ctaatttagt cttattagat tcaaactttg catcaatgcc aaaggttgtt
     6541 atggagggaa gaaaagtaat taataacatt caaagagcat cttctttgtt tttagtaaag
     6601 acaatttttt cattcttctt gtcgttatta acattattct tcttcagtag acagtatcca
     6661 tttgttccta tacaattatc actcattggg gctgtaacag tgggtatacc atcattcttc
     6721 ttagctcttg aagcaaacaa aagtagaatt tcaggaaatt ttttactcaa tatattaaaa
     6781 aatgctttac caggagcact atgtgtgaca gtaaatgtaa ttattgttta caatatagtt
     6841 gaatatttag gttatagttc aagtgtattt tccacaatgt gtgctatatt aactggtgta
     6901 tgtggtcttt taatattaag acaacaatgt ttgccattta ataaagaaag attattttta
     6961 atagtaagta tgactattgc atttgttatt gggattctat ttttaggtgg attattctca
     7021 tttgtagcat tgagtagaga attaattctc ataactatgg cattagcaat attaatgcca
     7081 attatgataa aagttatgtt atttgtctta gatgagatat tagagacaat tgttccagat
     7141 ttaaattaat tttaaaatga tataaataag agcagtttta gttgaactgc tctttttaga
     7201 tgtgtttaat tttatgtaag aatatgtatt tttattaatc agctatatat gagcaacagt
     7261 agtcaacaac ttcattaacc tttaagtcaa aacttgaatc actaggaaca ttaaattttt
     7321 gcccttcttt ataagttaca aactcattgc ttccaggcag ttttacatcc atacttccag
     7381 caagcatctc cattatttct tcttctctag tagaaaaagt gtattctcct ggcaacatta
     7441 atcctaaagt ttttctttct ccattaggca gtattattac tctgctactt accttaccat
     7501 caaaataaac gtttgccttc tttactgcag taacattctt aaattcactc attagataaa
     7561 acctccaaaa ttatttgtac atatatatta tataaaaat
//
LOCUS       DAMJYR010000165         7549 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.170, whole genome shotgun sequence.
ACCESSION   DAMJYR010000165 DAMJYR010000000
VERSION     DAMJYR010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7549
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.170"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            737..1588
                     /locus_tag="PC471_003396"
     CDS             737..1588
                     /locus_tag="PC471_003396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3100 domain-containing protein"
                     /protein_id="HDF2790482.1"
                     /translation="MEINKKHFIQVFLTCLILVVICEFIGQRTIAIGKASIVLFPMLY
                     AVIIGLIITPDLLGKKIKALKKVVGEKEINIAGEVVGIALVMLGIKYGTTVGPNIDKI
                     IQAGPAFIAQEFGHILAPIVALPLALMFGMKREAVGATASISREPSLGVISEKYGINS
                     PEGSGVLGTYLMGTVLGTIYFSVLGSISIYSGLHPYALGMACGVGSGSMMTAAAGSLS
                     TMVPPEMADTILAYAATSNMMSSITGIIFLVFVSLPFTNFMYKILEPKFSRNKKTKEI
                     KSTEGEC"
     gene            1592..2062
                     /locus_tag="PC471_003397"
     CDS             1592..2062
                     /locus_tag="PC471_003397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2790483.1"
                     /translation="MFANLNLKKSSYQLLVMSVIGVIILIGQRISVGTSIVTALPGMV
                     MLILAAMAAMIIKDLFPKSIFPAFGFATIIGLLLSMPYSPTSEVFLTNTNNINFMAIT
                     TPLLAFAGISVGNKIEALKEMSWKIVIISLIVFTTIFFACASIAHIVLSIQGKI"
     gene            2075..3385
                     /locus_tag="PC471_003398"
     CDS             2075..3385
                     /locus_tag="PC471_003398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HDF2790484.1"
                     /translation="MNKEQLKSLVIKTIDENREKILKIGQGIYKNPEYGYKEFKTTEA
                     VSNFFEQELNLSVEKNIAVTGCKADANSSKKGPHISILGELDGISCKEHKDANEIGAS
                     HTCGHNIQIAGMLGAAVGLVKSGVLEHLDGKVSFMATPAEEFIELEYREQLKKEGKIT
                     YFGGKQELIKRGYFDDIDISMMFHASDLGEDKALVGPESNGFVGKKVKFIGKESHAGS
                     APHDGINALNAAMLAINNVNALRETFKESERVRFHPIITKGGDIVNVVPADVHMESYV
                     RARTINGMIDANERINKALMAGGMAVGADVEITEIPGYLPILRYKSLDNLFKNNLEDL
                     GLKGKVIDGGDFTGSFDFGDVSHIMPTLHPMIGGVKGALHTRDFEIVDEDLAYIIPAK
                     AMALTVVDLLFDNAKDAKDILDTFTPVMTKEEYLTFLEKHDKTL"
     gene            3597..4343
                     /locus_tag="PC471_003399"
     CDS             3597..4343
                     /locus_tag="PC471_003399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDF2790485.1"
                     /translation="MFNAGNRIINTWLYPIQDGFVLIDTGYENGFKHFKKRISKFNIK
                     IKDIRYIFLTHAHDDHVGFLNQILDESPNTKIVMHDKALEILYKGQNSFIGGCTSRTA
                     FLFCQIMKLFGKGEHSFSPLKQEFEKRCILVSDKNRKEIEKELNGKIIDTPGHTDDSI
                     SLLIDEGVLFCGDSAMNGFPSTHKITIFAESKLEFIQSWKTIINTKPKMIYPGHGKPF
                     EYSKLEQNLIYVNKINLYPLHNSKDIHRFL"
     gene            4705..6246
                     /locus_tag="PC471_003400"
     CDS             4705..6246
                     /locus_tag="PC471_003400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HDF2790486.1"
                     /translation="MNDNVVKKKNFFDRTLNRIETVGNKLPDPVTIFLGLCVLLLILS
                     SLVGSMGISVVHPGTGETITAVNLLTVEQLQILLGNIVSNFQGFAPLGLVLVTMIGAG
                     VCDKTGLMTATIKASVSKIPETRVTLVVMTIGMLANIASDAGTILFPPLAALVYLGVG
                     RHPLIGLFSGYAAVCLGFAANIMISVNDILAASFTVPAAQMIDSGFQANATMNLFFMI
                     ASTFVLILLATWVTEKIVAPRFGKYEGNAELDVDGSLSDVEKKGLKKASISILIYVAI
                     IVALSVIGKKPFLADPETGDLLSNNAPLMKGMVPIIALAFFIPGVVYGKTIGKIKKDK
                     DVVSIMASAMSDMGGYIILAFAASQFLQLFTNSNLGLILAVKGGEFLKSAGINGPLLL
                     IGFIILSCFINLFIGSASAKWAILAPIFVPMFMMVGYNPALTQMAYRIGDCITNPLSP
                     LFPYFPIILAFTRKYDKDAGMGTVIANMIPYSISFLIVWTILLILFMVFNIPLGPGIL
                     PSYSM"
     gene            6281..7387
                     /locus_tag="PC471_003401"
     CDS             6281..7387
                     /locus_tag="PC471_003401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HDF2790487.1"
                     /translation="MLDKNRLINNFMDMVRIDSPSNQELEMSKWLVNYLKERNIDAII
                     DDAGEKYGGNTGNVIAYIKGEEGSRPLCLCAHMDQVQPCLGVKPILDGNVVRSDGTTT
                     LGADDKAGIAAILEALEHVITEKIPHRDIYLCFTICEEAGMHGVKNFNPDNLPCKDMV
                     ILDSGGAIGSIAYKAPAQQSIKISFQGKKAHAGIEPEKGLNAILVASHAISNMHIGRI
                     DSLTTSNIGEIEGGGATNIVTDKVTLTAEIRSHIPETLEYELNHMEKCCKDAASKFNT
                     TYTFEHNMSYPSFELSRDSHVFKLSEEAIRQVGVVPNPMVIGGGSDANILANLGYNCA
                     ILSLGMYDVHTVNEYVNIDELYDTAKIVYHMIKL"
BASE COUNT         2645 a         1011 c         1234 g         2659 t
ORIGIN      
        1 atctcatttg ttatcgtctt acttaattta cttatttcta tatcaattca cttattatta
       61 cctcacttgt tatcatctta cttaatttac ttatttctat atcaatttac ttattatcat
      121 ctcatttgtt atcgtcttac ttaatttact tattgctatg tcaattcatt tattattatc
      181 tcacttgtta tcgtcttact taatttactt atttctatat caattcactt attatcatct
      241 taattcattt gcttattatc atttacttta ttcaatttct atatcaaaat tagtatttgt
      301 acttaatact taaaattaat ttaaagtctt tacatattat ttattttcta ttgtaatatt
      361 catttccata ctttactaag ctacttccac tcatttattt taaaatttca atttactatt
      421 tctcaactct atattattat ttttcaattt tatattacta attttaaagc tttttagcta
      481 ttattcctac aatatttaaa caattttttc ataataaagc tatattacaa tatattcaaa
      541 atatgtaata ttttagatat ttttcgttga caatgttaaa tttttgtttt atccttaatt
      601 aaggacatgt tattgacatt tctgaaactt atttgaaata gttttttcat tttatttagg
      661 aaaacgtatt atacttaaga aaacgtattt tctcatttca gataagtaaa atatttattc
      721 ttttggggag gaaatcatgg aaattaataa aaaacatttt attcaagtat ttttaacttg
      781 cttaatcttg gtagtaatat gtgaatttat agggcaaaga actatagcta ttggaaaagc
      841 ctctatagta ctgttcccta tgctttatgc agttataatt ggacttataa taacaccaga
      901 tttattaggt aaaaaaatta aagcacttaa aaaagtggtt ggagaaaaag aaataaatat
      961 tgctggtgaa gttgttggaa ttgcactagt aatgcttggt ataaaatatg gtactacagt
     1021 tggaccaaac attgacaaaa taattcaagc tggaccagcg tttatcgctc aagaatttgg
     1081 tcatatactt gcaccaatcg ttgcattacc tcttgcatta atgtttggaa tgaaaagaga
     1141 agctgttgga gctactgcaa gtataagtcg tgaaccttct ttaggagtta tatcagaaaa
     1201 atatgggata aactcaccag aaggtagtgg tgtacttggt acatatttaa tgggaacagt
     1261 tcttggtact atatacttta gtgtactagg ttcaatatct atttatagtg gtcttcaccc
     1321 atatgctttg ggaatggcct gtggtgttgg tagtggaagt atgatgacag cagctgctgg
     1381 ttcattatct accatggtac cacctgaaat ggcagataca atacttgcct atgctgcaac
     1441 aagcaatatg atgtctagta taacaggaat tatattctta gtttttgtat ccttaccatt
     1501 tactaatttt atgtataaga ttttagaacc taaattttcg agaaataaaa aaactaaaga
     1561 aattaaatct acagaaggag agtgctaata aatgtttgct aatttaaatt taaagaaaag
     1621 ttcatatcaa ttattggtta tgtctgttat tggagttata attcttatag gacaaagaat
     1681 tagcgttggt acatctatag ttacagctct tcctggtatg gtgatgctaa tattagctgc
     1741 tatggcagct atgattataa aagatttatt tcctaaatca atctttccag cttttggatt
     1801 tgcaactata atcggccttt tacttagtat gccatatagt ccaacatctg aagtattctt
     1861 aactaacact aataatatta actttatggc tataactact ccactactag cttttgcagg
     1921 aatttctgtt ggaaacaaaa tagaagcttt aaaagaaatg tcatggaaaa tagttataat
     1981 ttcacttatt gtatttacaa ctatattctt tgcatgtgcg agtatagctc atatagtttt
     2041 aagtattcaa ggtaaaatat aggaggaatt tattatgaat aaagaacaat taaaatctct
     2101 agtaataaaa actatagatg aaaatagaga aaaaatatta aaaataggtc aaggaatata
     2161 caaaaatcca gaatatggat ataaagaatt taaaactaca gaagctgttt caaacttttt
     2221 tgagcaagaa ttaaatttat ctgtagaaaa aaatatagca gttacaggat gcaaagcaga
     2281 tgcaaattct agcaagaaag gtcctcatat atctatactt ggagaattgg atggtatatc
     2341 atgtaaagaa cataaagatg ctaatgaaat aggtgcttct catacttgtg gacataatat
     2401 tcaaatagct ggaatgttag gtgctgctgt tggtttagta aaaagtggcg ttttagaaca
     2461 tttagatggt aaagtatctt ttatggctac tcctgctgaa gagtttattg agttggaata
     2521 cagagagcaa ttaaaaaaag aaggaaaaat aacttatttt ggtggaaaac aagaattaat
     2581 aaaaagaggt tattttgatg atatagacat atctatgatg tttcatgctt ctgatttagg
     2641 agaagataag gctctagttg gtcctgagag taatggattt gttggaaaaa aggttaaatt
     2701 tataggtaaa gaatctcatg ctggttctgc tccacatgat ggaataaatg ctttaaatgc
     2761 agcaatgctt gctataaaca acgtgaatgc acttagagaa acttttaaag agtctgaaag
     2821 agttagattc caccctatca tcacaaaagg tggagatata gtaaatgttg ttcctgctga
     2881 tgttcatatg gaatcatatg ttagagctag aactataaat ggtatgattg atgctaatga
     2941 aagaataaat aaggctttga tggctggtgg aatggcagtt ggagctgatg ttgagataac
     3001 tgaaattcct ggatatcttc caatattacg atacaaaagt cttgataatt tatttaaaaa
     3061 caatcttgaa gatttaggtc taaaaggaaa agttattgat ggtggagatt ttacaggctc
     3121 ttttgatttt ggagatgtgt ctcatataat gcctactctt caccctatga ttggtggtgt
     3181 aaaaggagct cttcatacaa gagattttga aatagttgat gaagatttag cttacataat
     3241 tcctgctaaa gcaatggcac ttacagttgt agaccttctt tttgataatg ctaaggatgc
     3301 taaagatata ttagatacct ttactccagt tatgactaaa gaagaatatc taacattctt
     3361 agaaaaacac gataaaactt tataagtcta tttttataat agggtggtat ctactgtaaa
     3421 agttatacca ctctttgttt ttgtaacagt atacttcttt tcttttagtt tttagattaa
     3481 actaaaatat attatcctcc ttaaaacttt tagagctacg ttgaaatatc agtagaacta
     3541 tgctatattc tttctaatta atataaattt ctattctaat tcaaggtggt gaaaaaatgt
     3601 ttaatgcagg aaatcgaatt ataaatacat ggttgtaccc tattcaagat ggctttgtct
     3661 tgattgatac tggatatgaa aatggtttta aacatttcaa aaaacgaatt tctaaattca
     3721 acataaaaat caaagatatt cgatacatat ttctaactca tgctcatgat gaccatgttg
     3781 gttttctaaa tcagattcta gacgaatctc ctaatacaaa gattgttatg catgataaag
     3841 ctttagaaat tctatataaa ggtcaaaact cttttattgg tggttgtacc agccgtactg
     3901 cctttctctt ttgccaaatt atgaaactat ttggaaaagg tgagcatagt ttttctccac
     3961 ttaaacaaga atttgaaaaa aggtgtattc ttgtgtctga caagaatcgt aaagaaattg
     4021 aaaaagaact aaatggaaaa attatagata caccaggaca tactgatgat tcaatttcac
     4081 tactaattga tgaaggtgtt ttattttgtg gagattctgc aatgaatgga tttccaagta
     4141 ctcacaaaat taccatattt gcagaaagca aactagaatt tattcaatct tggaaaacaa
     4201 tcatcaatac aaaacctaaa atgatttatc ctggacatgg aaaaccattt gaatacagta
     4261 aattagaaca aaatctaatc tatgtaaata aaataaattt atatcctcta cataattcaa
     4321 aagatattca cagatttttg tagaaaatac aaagtaaatt tatttgatta attaaaaaat
     4381 ctaacacttt actacgctac ttattttaat aacttaatct tccactagct tttcataaaa
     4441 ttttgtttta aaattaattt taaagtgcat atgttattta aatacattag ataaaattcg
     4501 tcattaatct acttaaagca tatttaacta acaccactca accagaaaat tcagaaaact
     4561 gcattgacac aggaaaaagt ttattttata ctttttttag gacatattgt ggacacgtct
     4621 taaatcgaca aacagaattt aaattgtaag attaaccaca atacttattt tttaattata
     4681 tttttaaaca ttagggagga tattatgaat gataacgtag ttaagaagaa aaactttttt
     4741 gacaggacat taaacagaat agaaactgtt ggtaataaac taccagaccc agttacaata
     4801 tttttagggt tatgtgtact tttacttata ctatctagtc tagttggttc tatgggtata
     4861 tcagtagttc atcctggtac tggagaaact ataaccgctg taaatttatt gactgtagaa
     4921 cagcttcaaa ttcttttagg aaacatagtc agcaatttcc aagggtttgc tcctttgggt
     4981 ctagttctag taactatgat aggtgctggt gtatgtgaca aaacaggact tatgacagca
     5041 acaatcaaag catcagtaag caagattcct gaaacaagag taactcttgt tgtaatgact
     5101 ataggtatgc ttgccaatat agcttcagat gcaggtacaa tattatttcc accattagct
     5161 gcacttgtat atcttggtgt tggtagacat ccacttatag gtttattttc tggatatgca
     5221 gcagtctgtc taggatttgc cgcaaacatt atgataagtg tcaatgatat acttgctgca
     5281 agttttacag ttccagcagc tcaaatgatt gattctggat ttcaagccaa tgcaacaatg
     5341 aacttatttt ttatgatagc atcaactttt gttctaatac ttttggcaac atgggtaaca
     5401 gaaaaaattg tagctcctag atttggtaaa tatgaaggaa atgcagaact agatgttgat
     5461 ggaagtctta gtgatgtaga aaagaaaggg cttaaaaaag ctagtataag tatattaata
     5521 tatgtggcta ttatagtagc tcttagtgta atcggtaaaa aaccattctt agctgaccca
     5581 gaaacaggag atttactatc aaacaatgct cctcttatga aaggtatggt acctataata
     5641 gcactagcat ttttcatacc tggtgttgta tacggaaaaa ctataggtaa aataaagaag
     5701 gataaggatg tagtctctat aatggctagt gcaatgagtg atatgggtgg atatataata
     5761 ctagcatttg ctgcttcaca atttttgcaa ttattcacaa atagtaattt aggtctgata
     5821 ctagctgtaa agggcggaga atttttaaaa tcagcaggaa taaatggacc tttattgttg
     5881 ataggattta ttatattatc atgttttata aatttattta taggtagtgc atctgcaaag
     5941 tgggctattc ttgcacctat atttgtacca atgtttatga tggttggata caatccagca
     6001 cttactcaga tggcttatcg tataggtgat tgtataacaa atccacttag cccattattt
     6061 ccgtacttcc ctattatact agcattcaca agaaaatacg ataaagatgc tggtatgggt
     6121 actgtaattg ctaatatgat accatactct atatcattct taatagtgtg gactatacta
     6181 ttaatattgt ttatggtatt taatattcca ctaggacctg gaatacttcc ttcatatagc
     6241 atgtaattta tttaaatctt tctaaaagga gaaataattt atgttagata aaaatagact
     6301 tataaataat tttatggata tggttagaat tgatagccca tctaatcaag aattagaaat
     6361 gtctaagtgg ttggtaaact acttaaaaga aagaaatata gatgccatta tagatgatgc
     6421 aggtgaaaaa tatggtggta atacaggtaa tgtcattgcc tatataaaag gtgaagaagg
     6481 aagtagacca ctttgtttat gtgcacacat ggaccaagtt caaccttgtt taggtgtaaa
     6541 accaatacta gatggtaatg tagttagaag tgatggaact actacacttg gtgcagatga
     6601 taaagctgga atagctgcta tacttgaagc tttagaacat gttattacag aaaaaatacc
     6661 acatagagat atttatcttt gttttacaat ctgtgaggaa gctggtatgc atggtgtcaa
     6721 aaactttaat cctgacaact tgccttgtaa agatatggtg attttagatt ctggtggagc
     6781 tattggttcg atagcataca aagctcctgc tcaacaatct ataaaaatct cttttcaagg
     6841 aaagaaagca catgctggta tagaacctga aaaagggtta aatgctatat tagtagcctc
     6901 tcatgcaata agcaacatgc acataggcag aatagattca ttgactactt caaatatagg
     6961 tgaaatagaa ggtggtggag ctacaaacat agtaactgat aaggttacat tgactgctga
     7021 aattaggtct catatacctg aaactttaga gtatgagctt aatcatatgg aaaaatgttg
     7081 taaggacgct gcatctaaat ttaatactac ttatactttt gaacataata tgtcttaccc
     7141 tagttttgaa cttagtagag atagtcatgt tttcaagtta agtgaagaag caataagaca
     7201 agttggtgtt gttccaaatc caatggtaat tggtggtgga agtgacgcaa atatattagc
     7261 taatcttgga tataattgtg ctatattaag tctaggtatg tatgatgtac acacagttaa
     7321 tgaatatgta aatatagacg aattatatga tactgccaaa atagtttatc atatgattaa
     7381 attataaata tcataataga actctttaaa ttataaaaat aaaaacctat gtatcctcaa
     7441 ctttataagt tatatattag agttgtttta cataggtttt agtattttta tataataatt
     7501 tgttttaaca tttttcataa taagctttga tattttatat agtaatttg
//
LOCUS       DAMJYR010000166         7350 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.171, whole genome shotgun sequence.
ACCESSION   DAMJYR010000166 DAMJYR010000000
VERSION     DAMJYR010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7350)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7350)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7350
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.171"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            631..1140
                     /locus_tag="PC471_003402"
     CDS             631..1140
                     /locus_tag="PC471_003402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009528606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HDF2790488.1"
                     /translation="MSKELAINEQIKDKEIRVLSPTGEQLGVMPTKEAQAMANSKNLD
                     LVQISPNANPPVCKIMDYGKFRYEQARKEKEAKKKQKTIVVKEVRLRPGIEQNDLNTK
                     ANNAIKFLKKGDKVKVELRFRGRELGHKDIGKEVMLKFLDIIKEFGEPTKAPAFEGNN
                     MVVIIDPKK"
     gene            1168..1362
                     /gene="rpmI"
                     /locus_tag="PC471_003403"
     CDS             1168..1362
                     /gene="rpmI"
                     /locus_tag="PC471_003403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011721840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HDF2790489.1"
                     /translation="MPKMKTHRGAAKRLKKTGTGKLKRAKAFKKHILTKKSAKTKMNL
                     RKSTLVSDGDAKRIAQLLPY"
     gene            1418..1774
                     /gene="rplT"
                     /locus_tag="PC471_003404"
     CDS             1418..1774
                     /gene="rplT"
                     /locus_tag="PC471_003404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HDF2790490.1"
                     /translation="MARVKKAMNARKKHKKILKLAKGFRGSRSKLYRPANTFVMKALK
                     NAYIGRKLKKRDFRKLWIQRINAAARMNGISYSRLMNGLKLSGVEVNRKMLSEMAIQD
                     PEGFAKLAEVAKAKLA"
     gene            2084..2629
                     /locus_tag="PC471_003405"
     CDS             2084..2629
                     /locus_tag="PC471_003405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2790491.1"
                     /translation="MNEKLMDCFVNPIKCKLLIEIFSNNRTTAKQLGEKFQDISKATL
                     YRYLNKMLKDDIIKVVEENKIRGTVEKVYAMKLDIADDVNKIIDENSGEEYAQLFTQY
                     MMGFLREFYEYAERKEKDILKDGSGFSVCPVYASIDEIVEVSKKIASILEPLRNNSHE
                     EGRRMHSIGVIITPPKKDEGK"
     gene            3064..4989
                     /locus_tag="PC471_003406"
     CDS             3064..4989
                     /locus_tag="PC471_003406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HDF2790492.1"
                     /translation="MKIKFVIMPLVIFLMVLFVGCQSTKDTEVKAKDTEIKILGTGDL
                     HSILTDSMVSYVNEEREKNKNLLMVDAGDFYGTQSREMWEWSSGKKLINIKRNGRAEY
                     DDIIKSSKDEVPIVKDMAKLKYDAVVLGDNEFVSNDKQSLDKLVSDFKNNNMPLVSAN
                     IYEQSGENYVQPYVMKNIKTDEGNVKVGILGLTIKEVGETLGLEDFEKDKKARELGEQ
                     ADYKGKLYANDLVEDAKKWIKVMEKESPDIIVAVVHSGEEPKTDRNPGNRIKELATTV
                     DGIDAIVAGHTHEKIDEHKYKNKSGDEVIVTQPGWHGDRVSEINFKLNKENGKWDIKE
                     KSSKLKIIDKEVSIVATADLHSHFSDKLTVDLVNERSNPIEPVVVDAGDFLDPQTDEM
                     TQWYKEWKSIRDNNSDKTISRCPMVMDMNQGMYDAVVLGNHEFVSEKDEFWSDERLLD
                     AVIEDFEQVAISVLSANIYRQSGKNYVKPYIIKDVETSEGNVKVGILGLTIKEVGKGL
                     KNVNLQEQFQYKDKLYANDLVEDAKKWVKVMKKKKPDIIVAVVHSGEEPKNPKHPGNR
                     IKELATTVDGIDAIVASHTHEKIDEHEYKNKSGEKVIVTQPGEHGKYYSKITFKVNKE
                     KGSWTINDKFSKTIEVE"
     gene            5390..7294
                     /locus_tag="PC471_003407"
     CDS             5390..7294
                     /locus_tag="PC471_003407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HDF2790493.1"
                     /translation="MKIKFIIVASIIFLIVLLVGCQSTKDTEINVLATSDLHSIIPDN
                     LISYVEEERKYDENLLMVDAGDFFDMQSGEMNKWFTGQKLVRFKDGIPEYKKIAEPRE
                     GEVPLAKKMAKLKYDAVTFGNHEFVSNDKQSLDKLVSDFKNNNIPLVSANVYEQSGEN
                     YVKPYVMKNINTDKGTVKVGILGLTIKEVGERSRFKSFEQDKKARELGEQPQYKGKLY
                     ANDLVEDANKWVNVMEKKEKPDIIVAIVHSGEEPKNPKNPGNRIKELATTVEGIDAIV
                     AGHTHEKIEQHTYKNNSGEEVIVTQPGAHGNSISKINFKLDKKNDKWFIKDKYSELKV
                     FGKEVNIITTSGLNPKFSDEMAVNLFNERDRDVQPIFIDTGDFLNTNTKEMTKWAKEW
                     QYNENKGIYKKINEWPIVYLGYDAVVLGSHEFTEDKETFEEKKSTLDYIIENFEEMKI
                     PVLSANIYDESGENYVKPYIIHNVETREGNIKVGVLGLTTTDKDEEDLSNSSLSKDSK
                     TSKIPEETGKLYTNNLVKDASEWVKVMQKESPDVIVAVVHSDNNSTLKNPSSEVKKLA
                     MSVDGIDAIVVGHTKNKVEEHIYKNKSGDEVIVTQSGEDCYSKISLELRKENGKWNII
                     NKYSKAIKMK"
BASE COUNT         3088 a          722 c         1388 g         2152 t
ORIGIN      
        1 atattagaat ataatgtagg acttgttatg tagtatagta acatattaga atataatgta
       61 ggatttgtta tgtagtatag tagcatatta gaatataatg taggacttgt tatgtagtat
      121 agtaacatat tagaatataa tgtaggattt gttatgtagt ataatagtat attagagtac
      181 aatatagaat ttgttatgta gtatagtagt atattagaat acaatataga atttgttggg
      241 tagtatagtg ttatattaga atacaatata ggatttgtta tgtagaatat atagagtcat
      301 gtagaatata catatataaa aggattttgg gtataaattt aaaaattaat agcaaaatga
      361 gtagtataga catataatta gtagtaggaa gtaattaaat ttttcctaaa caaaaaaaat
      421 agttgcaaat aatttgatta agtgatatag taaataaggt aagaataaaa caaagaagaa
      481 gtccgcttct caccttacgc cggaggttag taaggtaaat ataagttaaa gcttaatttt
      541 aatttaagtt tgtttaaatt tggtaactta tattgcggat gattacgtca tccgcttttt
      601 attttataaa aattttggag gtgtcctaca attagcaaag aattagcaat caatgaacaa
      661 ataaaagata aggaaataag agtcctttca ccaacaggtg aacaacttgg agtaatgcct
      721 actaaagaag ctcaagcaat ggctaatagc aaaaacttag atttggttca aatctcgccg
      781 aatgcaaatc caccagtatg taagataatg gattatggaa agtttagata tgagcaagcg
      841 agaaaagaga aagaagctaa gaaaaaacaa aaaactatag ttgtaaaaga agtaagactt
      901 agaccaggta ttgaacaaaa tgatttaaat acgaaagcta ataatgctat aaagttcctt
      961 aaaaaagggg ataaagtcaa agttgaactt agatttagag gaagagaatt aggacataaa
     1021 gacataggta aagaagttat gcttaagttt ttagatataa taaaagagtt tggagaacca
     1081 acaaaagccc ctgcatttga gggtaataat atggttgtaa ttatagatcc aaaaaaatag
     1141 atgattaact gagaggagga atttgtcatg cctaaaatga aaactcatag aggtgcagca
     1201 aagagattaa agaaaacagg aactggaaag ttaaagagag ctaaagcttt taaaaaacat
     1261 atattaacta aaaaatctgc aaaaactaaa atgaacttaa gaaaatcaac tttagtaagt
     1321 gatggagatg ctaagagaat agcacaatta ttaccatact aagatacaga ttgtaaataa
     1381 taactataga agagggatat aaaaggaggt cataacgatg gcaagagtta aaaaagctat
     1441 gaacgctcgt aaaaagcata agaaaatatt aaaacttgca aaaggattca gaggatctag
     1501 aagcaaatta tatagaccag caaatacatt cgtaatgaaa gcattaaaaa atgcttatat
     1561 aggaagaaag ttaaagaaga gagacttcag aaaactttgg atacaaagaa taaatgcagc
     1621 tgctagaatg aatggaatat cttattcaag attaatgaat ggattaaaat tatctggtgt
     1681 tgaagttaac agaaaaatgt tatctgaaat ggctatacaa gacccagaag gatttgcaaa
     1741 attagcagaa gttgctaaag caaaattagc ttagtattga atcatgagat gtaaagtctt
     1801 tgagttatat aaataattca aggacttttt tattttttga aaaatactta ttagtgtcat
     1861 gtacaaccaa ttttataatg ataaataaaa taagcaatac aatagaattt tatgagattt
     1921 attataagaa ttgttacaat tatttatgat agctataaaa agtcatagta aaaatgatat
     1981 tgatgtatgt tgatatatgt ataaagtttc caatcaatct tgaattaata ttatcaacat
     2041 gataatatta tcttattaat aatataaata agggggacat aatatgaatg aaaaactaat
     2101 ggattgtttt gtaaacccaa ttaaatgcaa gttattgatt gaaatttttt caaataatcg
     2161 aactacagct aaacaattag gagaaaaatt tcaagatata tcaaaggcaa cactttatcg
     2221 ttaccttaat aaaatgttga aagatgatat tataaaagtg gttgaagaaa ataaaataag
     2281 gggaactgtt gaaaaagtat atgctatgaa gttagatata gctgatgatg tgaacaaaat
     2341 tatagatgag aactctggag aagaatatgc tcaactgttt actcaatata tgatgggatt
     2401 tttaagagaa ttttatgaat acgctgaaag aaaggaaaag gatatcctaa aagatggttc
     2461 aggattttct gtctgtccag tatatgctag tattgatgag atagttgaag tgagtaaaaa
     2521 aattgctagt atactagaac ctctaagaaa taactcacat gaagaaggta gaagaatgca
     2581 ttctatagga gtgataatta ctccacctaa aaaggatgaa gggaaataaa tctgatttac
     2641 atggggtaga gccaatttat acagggaatc gttttatgat ttacatatgt atccagagca
     2701 aagtatctct gcttggattg tacttcattg taccatttca tccaatgact tggagtggta
     2761 caatgaagtt ttttaatttt tcatattaat gctaaagaaa tgaactttag accacatatg
     2821 caattaggtt gcattataca attagaccac atatacaaaa taaaaatcag tatttttata
     2881 gcagataaat tgactgtcat tcttcttaaa aatcatctat tagttattgt tagcatattg
     2941 aaagaaatta tagtaatcta ttgaataaat gaatattgcc tataatcata taaactgcta
     3001 tagtttaaaa taaagcagga cgttaaattt tatagtaatc taaaaattta agagggggat
     3061 tttatgaaaa taaaatttgt tattatgcca ttggtgatat ttttaatggt attatttgtg
     3121 gggtgtcaaa gtacaaaaga tacagaagtt aaagcaaaag atacagaaat taaaatatta
     3181 gggacaggtg atttacattc aattttgaca gactctatgg tttcgtatgt aaatgaggaa
     3241 agggaaaaga ataaaaatct gcttatggta gatgcgggag atttctatgg tacacaaagt
     3301 agagaaatgt gggaatggtc ttctggaaaa aagttaatta atattaaaag aaatggtaga
     3361 gcagagtatg atgacataat taagagtagt aaagatgaag tacctatagt taaagatatg
     3421 gcaaagttga aatatgatgc tgtagttttg ggagataatg aatttgtttc aaatgataag
     3481 caaagtcttg ataaactggt ttctgatttt aaaaataata atatgccatt ggtatcagca
     3541 aatatatatg aacaatctgg agaaaattat gtacaaccat atgtaatgaa aaatattaag
     3601 acagatgagg gcaatgtaaa agttggaata ctaggtctta ctataaaaga agtaggggaa
     3661 acccttggtc ttgaggattt tgaaaaggat aaaaaagcta gagagcttgg agaacaagct
     3721 gattacaaag gaaaattata tgcaaatgat ttagtagaag atgcaaaaaa atggataaaa
     3781 gttatggaaa aagaaagtcc agacataata gttgcagttg tacactcagg ggaagaacct
     3841 aaaacagata gaaatccagg aaatagaata aaagagttag caactacagt ggatggaata
     3901 gatgcaattg tagctggtca tactcatgaa aaaatagatg aacacaaata taaaaataaa
     3961 tctggagatg aagtaattgt tacacaacca ggttggcatg gagatagagt atcagagatt
     4021 aatttcaaac taaataaaga gaatggaaag tgggacataa aggaaaagtc tagtaaatta
     4081 aaaataattg ataaagaagt aagtatagtt gctactgcag atttacattc acatttctct
     4141 gataaattaa cagttgacct tgttaatgaa cgaagtaacc caattgaacc tgttgtagta
     4201 gatgcaggcg acttcttaga ccctcaaaca gatgaaatga ctcaatggta taaagagtgg
     4261 aaaagtataa gagataataa tagtgataaa acgattagta gatgcccaat ggtcatggat
     4321 atgaatcagg gaatgtatga tgcagtagta cttggaaatc atgaatttgt atctgaaaaa
     4381 gatgaatttt ggtcagatga acgcctttta gatgctgtta tagaggattt tgaacaggtg
     4441 gcaatttctg tgttatcggc aaatatatat aggcaatctg gtaaaaatta tgttaaacca
     4501 tatattataa aagatgtgga gacgagtgaa ggtaatgtaa aagtaggaat attgggactt
     4561 actataaaag aagtaggtaa aggtcttaaa aatgtaaact tacaagagca atttcaatat
     4621 aaggataaat tgtatgcaaa tgatttagtg gaagatgcaa agaaatgggt aaaagttatg
     4681 aaaaagaaaa aaccagatat aatagttgca gttgtacatt caggagaaga accaaaaaat
     4741 ccaaaacatc caggaaatag aataaaagaa ctagcaacta cagtagatgg tattgatgca
     4801 attgttgcta gtcatactca tgaaaaaata gatgagcatg aatataaaaa taagtctgga
     4861 gaaaaagtta tagtgacaca accaggtgag catggtaaat attattctaa aattaccttt
     4921 aaagtaaata aagaaaaggg ttcgtggact attaatgata aattcagtaa aacaattgag
     4981 gttgaataac tttataaaat ttatagataa agtaaattat ctgatattgg catgatatat
     5041 aagataaagt ttctaatatt agtataatgc ataatgtgaa atattttaat aataagagaa
     5101 taaacaagac aaataaaaat attttaacat gtaagatata gtatgaacta ttatactaat
     5161 gcttacttta taatttgaac atgttagaat aagtcttatt tgaaaatttt agtgtactag
     5221 agtaagtatt attttactag taaaaaatat tgagaactat atgtagattt gcttaaaaag
     5281 gaatttacat atagttcttt tcgtataata aatagtacaa tcttatatgg taaagataaa
     5341 gtgtgatatt aaattttatt atcatccaaa atctaaggag gagtctctta tgaaaataaa
     5401 atttattata gtagcatcaa taatattttt aatagtatta cttgtggggt gtcaaagtac
     5461 taaagacaca gaaatcaatg tactcgccac aagtgattta cattcaatta ttccagataa
     5521 tttaatttcc tatgtagaag aggagagaaa atatgatgag aatctactta tggtagatgc
     5581 aggagatttt tttgacatgc aaagtggtga gatgaataag tggtttactg gtcaaaaatt
     5641 agtaagattt aaagatggga taccagagta caaaaaaata gctgaaccac gtgaaggaga
     5701 agttccattg gctaaaaaaa tggcaaagct gaagtatgat gcagttacat ttgggaatca
     5761 tgaatttgta tctaatgaca agcagagcct tgataaattg gtttctgatt ttaaaaataa
     5821 caatatacca ttagtatcag caaacgtata tgaacaatct ggagaaaact acgtaaagcc
     5881 atatgtaatg aaaaatataa atactgacaa gggaactgta aaggttggta tacttggtct
     5941 gactataaaa gaagtagggg aacgttcaag gtttaaaagt tttgaacaag ataaaaaggc
     6001 tagagaactt ggggaacaac ctcaatacaa agggaaatta tatgcaaatg atttagtaga
     6061 agatgcaaat aagtgggtaa atgttatgga aaaaaaagaa aagccagata taatagttgc
     6121 cattgtacat tcaggtgaag agccaaaaaa tcctaaaaat ccaggaaata gaataaaaga
     6181 actagcaaca actgtagagg gaatagatgc tattgtagca ggtcatacac atgaaaaaat
     6241 agaacagcat acatataaaa ataattctgg tgaagaagta atagtaacac agcctggagc
     6301 tcatggtaat agtatatcaa agattaattt taagttggat aagaagaatg ataagtggtt
     6361 tataaaagac aaatatagtg aattaaaggt ttttggaaag gaagtaaata taattacaac
     6421 ttctggtctg aatccaaaat tttcagatga aatggcagtt aatttgttta atgaaagaga
     6481 tagagatgtt caacctatct ttatagatac tggagatttt ttaaatacta atacaaaaga
     6541 aatgactaag tgggctaaag agtggcaata caatgaaaat aaaggcatct ataaaaaaat
     6601 aaatgaatgg ccaattgtat atcttggtta tgatgctgta gtacttggta gccatgaatt
     6661 tacagaggat aaagagactt ttgaggaaaa aaaatctaca ttggattata ttatagaaaa
     6721 ctttgaagaa atgaagatac cagtattatc agctaatata tatgatgaat ctggagaaaa
     6781 ttatgtaaaa ccttatataa ttcataatgt agaaactagg gaaggaaata taaaggttgg
     6841 agtattaggt cttactacta ctgataagga tgaagaagac ttaagtaact caagcttaag
     6901 caaagactca aagacttcga aaattcctga agaaacagga aagttatata ctaataatct
     6961 agtaaaagat gcaagcgaat gggtaaaagt tatgcaaaag gaaagtcctg atgtgattgt
     7021 tgcagttgta cattcagata ataatagtac tctaaaaaat ccaagtagtg aagtcaaaaa
     7081 acttgcaatg tcagtagatg gaatagatgc tattgttgta ggtcatacga aaaataaggt
     7141 agaagaacat atttacaaaa ataagtctgg ggatgaggtt atagtaacac aatctggaga
     7201 agattgttat tcaaaaataa gtcttgaact aagaaaagaa aatggcaagt ggaatatcat
     7261 aaacaaatat agtaaagcaa taaaaatgaa gtgaatttat tgatatataa accattatta
     7321 acactgattt ttataatata ataaatactg
//
LOCUS       DAMJYR010000167         7341 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.172, whole genome shotgun sequence.
ACCESSION   DAMJYR010000167 DAMJYR010000000
VERSION     DAMJYR010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7341
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.172"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            159..3338
                     /locus_tag="PC471_003408"
     CDS             159..3338
                     /locus_tag="PC471_003408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HDF2790494.1"
                     /translation="MKIDMIYKLIRRNTEPSRWSRGVKYYTNQLVDYIDIDIDDLQNN
                     LLTFRGGVNSEDNYSFYFNSITIDIKHFKIIENDCSCADCQSKGKSIFICKHIVAIVI
                     KSLKQIKNKKDVPPILIDILGADKDKLSNTNSKTSLDLSKQLLSLLDNTKRERVNLDV
                     LINVDSNKAIAEFKIGINKMYILKNITEFAYSKLNGYELDFGKKFTYNPNTHYFADED
                     EKLVDIIEEYGTNLSYNFSSSHSKKFMEINASSLKQFLNAIKYKDFTLIFSKEEYHPK
                     ILNDTLPVRFDFKNIEDKIVLYSKDSLPVPLTKKNDVLLYDGNIYLLSHKKAHDYSKI
                     YQILSKTKELKFDKEDSKDVLGLLVPRLKSISQEVHLDDNIKNNITKDFKSEFYFDMI
                     DGNICCDVKYIYDDEDKKFVLPNIQKEATIENKLTSSQFTKENNHYVFKGTDHDLFTF
                     LDLQLESLKEFGDIYYSEKFKLKKIYNASSIQASINQTNQNYLEFNFNISDINPKEYK
                     DILKAFQEKRTFYKLKDGNFIDLSERETKDFFELVENLDIMDKSKNNKIHKNKALYIN
                     DVIRSKNLKFIDGKKELDSICEKFRNFDSINLEIPANLNANLRDYQINGLNWFKVLDY
                     YKFGGILADEMGLGKTIQTIAFLLSLSNKKSLIVTPTSLIYNWKNEFEKFAPDIKVLL
                     IHGNKRDREKCFMELENFDVILTTYGTLRNDLDKYSEIKFDYCILDEAQNIKNPVALV
                     TESVKSINAENKFALTGTPMENNLLELWSIFDFIMPGYLYSKAKFQELFINKEDNVKN
                     LKKLIKPFILRRSKKQVMKELPDKIEKNFFVELNKEQKKIYSVYSKDIQDKMKDKNLK
                     KDKIVIFSYLTKLRQLCLDPSIVVKDYNKKSSKIETCLEILRDSINENHKILLFSQFT
                     SVLKNISKELDKYKIKYHYIDGKTNAKERLELVDEFNNSMDKKVFLISLKAGGTGLNL
                     TSADMVIHFDPWWNPSVENQASDRAHRFGQKNSVQVIKLIAKGTIEEKIIKLQESKKE
                     LINQFINGELSNEGVLKSLSDEEIINLFN"
     gene            complement(3623..4387)
                     /locus_tag="PC471_003409"
     CDS             complement(3623..4387)
                     /locus_tag="PC471_003409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDF2790495.1"
                     /translation="MAYFNYNDKQCYYNEIGDGTPLLFLHGNTASSKMFNDIINFYKD
                     EYKVILIDFVGHGKSQMVDKFSADLWFDEAMQVICFLEAMNYKKVNIIGSSGGALVAL
                     NVALERPDLVNKVIADSFEGEVPLESFVQNVKIEREASKQDDGAKAFYIYNQGENWEK
                     VVDNDTEAIFEHYKTIGKFFHKPLETMQPEVLLTGSREDEFVSLISSDFFENTFSLLL
                     EKIKNGKMYLFDKGGHPAILSNGLDFSNVAKKFLEE"
     gene            complement(4543..4776)
                     /locus_tag="PC471_003410"
     CDS             complement(4543..4776)
                     /locus_tag="PC471_003410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2790496.1"
                     /translation="MKAEDMKRKVSPIDLERILVTGGVIKHEDSIWLLDFWGNKDIAG
                     ILLMPPTRHVILHLNDCCKWKEYFRTKKKFYRS"
     gene            complement(4850..6343)
                     /locus_tag="PC471_003411"
     CDS             complement(4850..6343)
                     /locus_tag="PC471_003411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HDF2790497.1"
                     /translation="MKYTFEKFKLNEKILKSLKSLGYNIPSRVQREVIPKLLKGQNLV
                     VRSKTGSGKTASFAIPLCENINVDYNNIQALIVVPTRELALQVKDEISDIGRLKKVRC
                     SAIFGKQSIKDQIAELKQRVHIVVATPGRILDHINRGSIKLENVKYLVIDEADKMFNK
                     GFVEQMEKILLNLPKEKIVSLFSATIDEEIKYICEKYMLDYSVINIEENESDTNQKTR
                     QIDDKIIKANGREKYILLKELIYSENPKSVIIFCNTKEKVSKLYNKMSKEGFLIRELH
                     ADLSQERRIFVIKDFKNQKFNILVSSDVASRGIHIDDISLVINYDVPQDKENYIHRIG
                     RTGRKGNSGKAITIVTEKDEKYIENIETYIGYKINELKDIEKSRITHGKVLFEEYSKG
                     ILKKVSKRKKVDKNSYKSKDIKLNIESEVVKLYLNAGKKKKIRVLDIVGAFSNIKGIT
                     NDDIGVIEVQDLCSYVDILNYKGDLILKKYKEIPIKKKMVKVKRDIR"
     gene            complement(6671..7213)
                     /locus_tag="PC471_003412"
     CDS             complement(6671..7213)
                     /locus_tag="PC471_003412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HDF2790498.1"
                     /translation="MKICITVGHSILKGGKSTGVNGIVDEYNYNKKLAPMLAEMLISQ
                     GNTVDVIICPERHFMSEREEFFYRVPKVNSGKYDLLVELHLNKADGTQCGTEVLYYGT
                     EGLEYAKRVSNRLGELFENRGAKKRENLYILRNTNPVAIQIESFFCDNVNDCNKANES
                     GYDYIARLITEGILNKDINM"
BASE COUNT         2691 a         1064 c          820 g         2766 t
ORIGIN      
        1 taagataatt caataaaaat ctaatagtaa attgttattt acacatatat cgtgatagat
       61 aaaagctatt catatctgcc ttaattttag tcttaaattt agagtgtaaa ttgaatgcat
      121 ttagattata atatatacag tgaggtaagg tgaataatat gaaaatagat atgatatata
      181 aactaataag aagaaataca gaaccatcaa gatggtcaag aggtgtaaaa tattatacta
      241 atcaattagt tgattatata gacatagata ttgatgacct acaaaataat ctgttgacat
      301 ttcgtggtgg agtcaattct gaagataatt atagctttta ttttaattca ataactatag
      361 atataaagca ctttaagata attgaaaatg attgtagttg cgctgactgt caatcaaagg
      421 gcaaaagtat ttttatatgt aagcatatag ttgctattgt gataaaaagc ttaaaacaaa
      481 taaaaaataa aaaagatgtt cctcctattc taatagatat attaggtgca gataaggata
      541 agctttctaa tacaaattct aaaaccagct tagatttaag caagcaatta ctttctttgt
      601 tagacaatac taaaagagaa agggttaatt tagatgtatt gataaatgtg gattcaaata
      661 aagcaattgc tgaatttaaa ataggaatta ataaaatgta cattttaaaa aacattactg
      721 aatttgcata ttcaaaactt aatggatatg aattagattt tgggaagaaa tttacttaca
      781 accccaacac tcattatttt gctgatgaag atgagaagtt agtagatatt atagaagaat
      841 atgggacaaa tctttcatac aacttttctt catcacattc aaaaaaattt atggagataa
      901 atgcaagctc tttaaaacaa tttttaaatg ctattaagta caaggatttt acccttattt
      961 tttctaaaga ggaatatcat ccaaagatat taaatgatac tctacctgta aggtttgatt
     1021 ttaaaaatat agaagataaa atagtcttat attctaaaga ttcactacct gttccactta
     1081 ctaaaaaaaa tgatgtactt ttatatgatg gcaatatcta tttattatca cataaaaaag
     1141 ctcatgatta ttcaaaaata tatcaaatat tatcaaagac taaagagtta aaatttgata
     1201 aagaggattc taaggatgtt ttaggattgc tagttcctag attaaaatct atatctcaag
     1261 aagttcattt agatgataac ataaaaaata atataacaaa agattttaaa tctgagttct
     1321 actttgatat gattgatggc aacatatgtt gtgatgtaaa gtatatatat gatgatgaag
     1381 ataaaaaatt tgtgctcccc aacatacaaa aggaagctac tattgaaaat aaacttactt
     1441 cttcacaatt tacaaaggaa aataatcatt atgtgtttaa aggaactgac catgacctat
     1501 ttacattttt agatttacaa ttagaatctc taaaggaatt tggagatata tattattctg
     1561 agaagtttaa attgaaaaaa atatataacg cttcatcaat tcaagcttca atcaatcaga
     1621 ctaaccagaa ttacctagaa tttaatttta atatatctga tataaatcca aaagagtaca
     1681 aggatatttt gaaagctttt caagaaaaac gtacttttta taaacttaaa gatggaaact
     1741 ttatagattt atcagaaaga gaaacaaaag atttttttga attagttgaa aatctagata
     1801 taatggataa atcaaaaaat aataaaattc ataaaaataa agctctatat ataaatgatg
     1861 taatacgtag taaaaattta aagtttatag atggtaaaaa agaactagac agtatatgtg
     1921 aaaaatttag aaattttgat agcataaatt tagaaattcc cgcaaattta aatgcaaacc
     1981 ttcgtgacta tcaaataaat ggcctcaatt ggtttaaagt tttagattat tataaatttg
     2041 gtggtatatt agctgatgaa atgggtcttg gtaaaacaat acagactatt gcatttttat
     2101 tatcattatc taataaaaaa agtcttattg ttacaccgac atcactcatt tataattgga
     2161 aaaatgaatt tgaaaagttt gctccagata taaaagtttt attgatacat ggaaataaaa
     2221 gagatagaga aaaatgtttt atggaattag aaaattttga tgtaatactt acaacatatg
     2281 gtactcttag aaatgattta gacaaatata gtgaaataaa gtttgattat tgtatattag
     2341 acgaagctca aaatataaaa aatccagtgg ctttagtaac tgagtctgta aaaagtataa
     2401 atgcagaaaa taaatttgca ctcactggta cacctatgga aaataatcta ctagaattat
     2461 ggtctatatt tgattttata atgcctggct atctatatag taaagcaaag tttcaggaat
     2521 tgtttataaa taaagaagat aatgttaaaa acctgaaaaa attgataaaa ccttttattc
     2581 ttagaagaag taaaaaacaa gttatgaaag aacttccaga taagattgaa aaaaactttt
     2641 ttgttgaatt gaataaagag cagaaaaaga tatatagtgt atattcaaaa gatatacaag
     2701 ataaaatgaa agataaaaat ctaaagaaag ataaaatagt tatattctct tatttaacta
     2761 aactaagaca actttgttta gacccaagta tagtagtaaa agattacaat aaaaaaagtt
     2821 caaaaattga aacatgttta gaaatactta gggatagtat aaacgaaaac cacaaaatat
     2881 tattgttttc acaatttact tctgtgctta agaacataag taaggaactt gataagtata
     2941 aaattaaata ccattatata gatggtaaaa ctaatgccaa agaaagactt gaacttgtag
     3001 atgagttcaa taatagtatg gataaaaaag tttttttaat atctttaaaa gcaggcggaa
     3061 ctggtttaaa tcttacatct gctgacatgg taattcactt tgacccatgg tggaatccat
     3121 cagtagaaaa tcaagctagt gatagagctc atagatttgg tcaaaaaaat tcagtacaag
     3181 ttataaaatt gattgctaaa gggactatag aagaaaaaat aataaaatta caagaaagta
     3241 aaaaagagct tataaatcaa tttataaatg gagaactttc taatgaaggt gtattaaaaa
     3301 gtctatctga tgaggagata ataaacttat ttaactaaat tatttaatcc tacagcttaa
     3361 ctatatgaat ttgttctaac atttatatag ttaagctaaa atatctgaat aaataaaatt
     3421 taatattgtt atttaatccc aactttacaa tctaatgata tctctttcta tacatattgc
     3481 atatacaaat gtaataactt gactattaga atattttgtt ttaacaatcc tatttcatca
     3541 taataatgaa gtatatgtac actcactttt gtaagttttg caatccattt tacagttttc
     3601 attattgtaa attttggtta aattattcct ctaaaaattt ctttgctaca tttgaaaaat
     3661 ctaatccatt cgataaaatt gcaggatgac cacctttatc aaacaaatac attttaccat
     3721 ttttaatctt ttcaagaagt aatgaaaaag tattttcaaa aaaatcacta gatattagag
     3781 atacaaactc atcttctcta cttcctgtta aaagcacctc tggttgcata gtttctagag
     3841 gcttatgaaa aaactttcct atagttttat aatgttcaaa aatagcttct gtatcattat
     3901 ctacaacttt ttcccagttt tctccttgat tataaatata aaaagctttt gcaccatcat
     3961 cttgcttaga tgcttcacgc tctattttca cattttgtac aaatgactct agaggaactt
     4021 ctccttcaaa actatctgca attaccttat tcactaaatc aggtctctca agtgcaacat
     4081 ttaatgcaac aagagctcct ccactactac caattatgtt tactttttta tagttcattg
     4141 cttctaaaaa acatatcact tgcatagcct catcaaacca taagtcagca gaaaatttat
     4201 cgaccatttg agattttcca tgacctacaa aatcaattaa tattacttta tattcatctt
     4261 tataaaaatt gattatatca ttaaacatct tggaagaggc tgtatttcca tgtaaaaaaa
     4321 gtaatggtgt accatcccca atttcattat agtagcactg tttgtcgtta tagttaaaat
     4381 atgccatata tttcccccct ttaatatccc tataggtata tatttattac ttatatttaa
     4441 aatgactatt cccttagcca cttaaaaata gtataactta gaatgtagat aaaatcaaca
     4501 ttccttgcta atttatctat tgtttgtcta atttattata atttaacttc tgtaaaattt
     4561 cttttttgta cgaaaatatt ccttccattt acaacaatca ttcaaatgaa gtataacatg
     4621 acgagtagga ggcataagca aaattccagc aatatcttta ttcccccaaa agtccaacaa
     4681 ccaaatagaa tcttcatgct ttattactcc tccagtcacc aaaatccttt ctaagtcaat
     4741 tggagagacc tttcgtttca tatcttcagc tttcaaatcc ttaacaactt ctattgtgtt
     4801 ttttgcaact tcatttcgat aacaaagcaa ttcatcaata ttgactaaat tatcgtatat
     4861 ctcttttcac tttaaccatt tttttcttta taggtatctc cttatatttt tttaatatca
     4921 aatctccctt ataatttaat atatctacat atgaacataa atcttgaact tcaattacac
     4981 ctatatcatc attagttata ccttttatat tactaaatgc accaactata tctaaaactc
     5041 ttattttctt cttcttacca gcatttaaat ataatttaac aacttcactt tctatattta
     5101 gttttatatc tttacttttg taagaatttt tatcaacttt ttttctcttt gatacttttt
     5161 ttaggattcc ttttgaatat tcttcaaaca aaactttgcc atgtgtaatt ctactttttt
     5221 ctatatcttt taattcattt attttatatc ctatatacgt ttctatattc tctatatatt
     5281 tttcatcctt ttcagttact atagtaattg cctttccact atttcctttt ctcccagttc
     5341 tacctattct atgtatatag ttttctttat cttgtggcac atcataattt attactaaag
     5401 aaatatcatc aatatgtatt cctctggacg ctacatcact actaactaat atattaaatt
     5461 tttggttttt aaaatcctta attacaaaaa ttcttctttc ttgtgataaa tctgcatgta
     5521 actctcttat taaaaaaccc tctttactca tcttgttata taatttagat actttctctt
     5581 tggtattaca aaatattatc acactctttg gattttcgct gtatattaat tcttttaaaa
     5641 ggatgtactt ctctcttcca tttgccttga ttattttgtc atctatttgt cttgtttttt
     5701 gattagtatc gctctcattt tcttctatat ttataacact gtaatctagc atatattttt
     5761 cacatatata ttttatctct tcatctattg tagctgaaaa aagacttact attttctcct
     5821 taggtaaatt taataatatt ttttccattt gttcaacaaa acctttatta aacattttat
     5881 ctgcctcatc tattactaaa tactttacat tttccaactt aatagaacct ctatttatat
     5941 ggtcaagtat tctgcctgga gtagctacaa caatatgaac cctttgtttc aattcagcta
     6001 tttggtcttt tatagattgt ttgccaaata tagcactaca tctcaccttc tttagtctcc
     6061 ctatatctga tatctcatct tttacttgca aggctaattc tcttgttgga accactataa
     6121 gagcttgaat attattgtaa tctacattta tattctcgca taaaggtatt gcaaaacttg
     6181 ctgtctttcc acttcctgtt tttgacctaa ctactaagtt ttgacctttt aataattttg
     6241 gaataacttc tctttgtact ctagaaggta tattatatcc taaactcttc aatgacttaa
     6301 gtatcttttc atttaactta aatttttcaa atgtatattt catatattac caccttatta
     6361 tatttatctt atctatatat ttttctacat tatattatta taatacaagg ttctactatt
     6421 tttatattat ttattagact gaagataaaa gtatatactc tataaaccta tagtttagta
     6481 gcaaattaat taaattttta tatttaaata cctccttaaa tatactgata aatcaatatt
     6541 ttcaaggagg tatctcttgt caataatcaa actaatctat cttttgaaag tttaaattat
     6601 actaatacat tgtattttta tttatatcta atattaaaag gtattaatca atacctaaat
     6661 caccataatc tcacatattt atatctttat tcaatatacc ttctgtaatc aatcgtgcta
     6721 tataatcata acctgattca tttgctttgt tacaatcatt cacattatca cagaaaaaac
     6781 tttcaatctg tattgcaact ggatttgtat ttctcaatat atataaattt tctctttttt
     6841 tagctcctct attttcaaat aattctccaa gtctattaga tactctttta gcatattcta
     6901 atccttcagt gccatagtaa agcacttctg taccacattg tgttccatca gctttattta
     6961 aatgtagctc tactaataaa tcgtatttac cactgtttac ttttggaact ctatagaaaa
     7021 attcttctct ttctgacata aagtgccttt ctggacaaat tattacatct actgtattac
     7081 cttgacttat aagcatctca gctaacatag gagctaattt tttattataa ttgtattcat
     7141 caactatacc atttacacct gtacttttac caccttttag tatactatga cctactgtta
     7201 tgcatatttt catttacttt tttcaccttc ttttaagcca atttttatat aatagtaata
     7261 taattttaaa gttattacta ataataatat attaattgaa gaaggtaaaa gttctccgtg
     7321 attatcattt ttttaacata t
//
LOCUS       DAMJYR010000168         7286 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.173, whole genome shotgun sequence.
ACCESSION   DAMJYR010000168 DAMJYR010000000
VERSION     DAMJYR010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7286)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7286)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7286
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.173"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(3..950)
                     /locus_tag="PC471_003413"
     CDS             complement(3..950)
                     /locus_tag="PC471_003413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HDF2790499.1"
                     /translation="MFYFLKVKVGDIMDEVKEFLVLEEEEEVRLDIYLAEQLGDMSRS
                     YIQKIIKDGKVKVNNKVEKARYLVKEEDNIVIEIPEPKVLEVLPQDIPIDIVYEDDDV
                     LIINKPQDMVVHPAPGNYENTLVNGILYHCKDKLSSINGVIRPGIVHRIDKDTSGLLM
                     IAKNNYAHNFLAEQLKEHTITREYEFICYGVVKEDKITVDKPIGRNPKDRLKMAVVKD
                     GKNAITHFEVVERFDKFTHMRARLETGRTHQIRVHALSINHPLLGDYVYGPKETKFKL
                     KGQTLHAKKLGFIHPTTKEYIEFNSELPEYFKEVIKKIK"
     gene            complement(919..1365)
                     /gene="lspA"
                     /locus_tag="PC471_003414"
     CDS             complement(919..1365)
                     /gene="lspA"
                     /locus_tag="PC471_003414"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HDF2790500.1"
                     /translation="MLYILIIILLIGLDQLSKIWVLNNLVDVSTIPIINNVFHLTYVE
                     NRGAAFGLLQNNQWIFIIVALLATVFGLYYLNTRKVHIFGRLGIILIISGALGNLVDR
                     VRLGFVVDYFDFRVIWEYVFNVADVFVVVGTVFLCIYVLFFESKSR"
     gene            complement(1675..2454)
                     /locus_tag="PC471_003415"
     CDS             complement(1675..2454)
                     /locus_tag="PC471_003415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HDF2790501.1"
                     /translation="MKKIICGICICLLSIVGVYTYNHNTHNQDSSKEAYSLNDNIKDD
                     YEDVIIKKGNEDEKILALTFDDGPDEDFTPQVLDILKKNDVKATFFVVGEKVEYNKEL
                     LKRQYDEGHEIGNHTFTHINVAKNSYSSVEKEITDTQNIIKSVIGVEPKIFRPPYRAM
                     SKSVCDIIVSKGMNIILWSNLDPRDWSNPGVDAIINTILTKVQNGNIILLHDYNTKRN
                     DKSQTIQALEVVIPKLKERGYKFVTVSELIQHLDKNKQVQK"
     gene            complement(2509..2991)
                     /locus_tag="PC471_003416"
     CDS             complement(2509..2991)
                     /locus_tag="PC471_003416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HDF2790502.1"
                     /translation="MDTKKYKDKLLKERENLTNLVEDMKDNTLFGDTTKHTSEKYSSG
                     ELSSYDNHIGDMGTDLYMQNMQNSLINHEEGRLYQIDLALSKIENGTYGICDLCHNQI
                     DLERLDILPDTSLCNDCAKKENNLLGDAMQNPDVEDSNFYSEDLTNLTDLNKNGILRD
                     "
     gene            complement(3257..4678)
                     /locus_tag="PC471_003417"
     CDS             complement(3257..4678)
                     /locus_tag="PC471_003417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein A"
                     /protein_id="HDF2790503.1"
                     /translation="MVSFLGSIVVLIVGYFVYGTFVEKVFGTNDKNQTPAIACRDGVD
                     YVPMKWKRIFLIQFLNIAGLGPIFGAIQGALFGPSAFLWIVFGTIFAGGVHDFASGYL
                     SMKNKGTSASELVGLYLGNGAKIAMRIFSVILLVLVGVVFVTGPAGLLKTLTGVNAQI
                     WVGVIILYYIMATVLPIDKLIGKIYPLFGAALLIMAVGVAGGLIIKGYNIPNINLQNM
                     NPNGTPLFPYLFITIACGAISGFHATQSPLMARCVEKESEARPVFYGSMVAEGIIALV
                     WAAAAMSYFHGIPQLNVIFNDGGAATVVNTVSVGLMGPIGGALAILGVVACPITSGDT
                     AFRSARLTIADAMNYNQDAVKNRFLIALPLFAVGVALTFIDFNIIWRYFSWANQTLAM
                     IMLWTGSAYLVKANKNHYITTLPALFMTVVTFSYIMQAKEGFRLPVNISNGIGVIVAI
                     ILGLLFFKKAKHIKEQNNHKLAS"
     gene            complement(4824..5594)
                     /locus_tag="PC471_003418"
                     /pseudo
     CDS             complement(4824..5594)
                     /locus_tag="PC471_003418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295458.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
     gene            complement(5599..6801)
                     /locus_tag="PC471_003419"
     CDS             complement(5599..6801)
                     /locus_tag="PC471_003419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HDF2790504.1"
                     /translation="MKTSILIALIEKTSLIVVLFLLITKLKIFKQIFQKEEYSFNDLV
                     CIALVFTFLAIFGTYSGINYMGSIVNTRIISIVSGGILFGPMVGITAGVFSGIHRYFM
                     DIGGITSVPCLLSSILAGVLSGFLYKRIPKQHRVMYGILVGMISESFTILLIYLISYP
                     HSLAIQIIGGIYLPLIVGQIGIGFVISIVEGIEKDKKDIEARNKAEIKALQRQINPHF
                     LFNSLNTIASFIRFSPDKARELIINLSTYLRYNLEYSDNLIDINKEIEQVKSFVEIEK
                     ARFGELLTVSYDIEDVNIKIPSLIIQPLVENAIIHGILESGRAGVVKISIKKLPYSLE
                     NTVRISIEDNGIGISEEIINNVYQDNMPENKIGLYNVHLRLKLMYGRGLNIRRIDNGT
                     LIVFYVKE"
BASE COUNT         2421 a         1275 c          709 g         2881 t
ORIGIN      
        1 ggttatttta tctttttaat aacttcctta aaatactcag gtaattctga attaaattcg
       61 atatattcct tagtagtagg atgtataaat cccaacttct tagcgtgcaa agtttgtcct
      121 tttaatttaa atttagtttc ttttggacca tatacataat cacctaaaag tgggtgattt
      181 atagacaaag catgaactct tatttgatga gttctaccag tttcaagcct agctctcata
      241 tgtgtaaact tatcaaatct ttctacaact tcaaagtgag tgattgcatt ttttccatct
      301 tttactacag ccatttttaa tctatcctta ggatttctcc ctataggttt atcaacagtt
      361 attttatctt ctttaaccac accatagcat ataaattcat actccctagt tatagtatgt
      421 tcctttaact gttctgctag aaaattatgt gcataattgt tcttagctat cataagaaga
      481 ccagaagtat ctttgtctat tctatgaact ataccaggtc taataactcc atttattgat
      541 gacaacttat ctttacaatg atataaaatc ccatttacta acgtattttc atagtttcca
      601 ggagcaggat ggacaaccat atcctgaggt ttatttatta ttaagacatc atcatcttca
      661 tatacaatat ctattggaat atcttgaggt aaaacttcta atactttagg ttctggtatc
      721 tctataacaa tattatcttc ctctttaacc agatatctag ctttttctac tttattatta
      781 acctttactt ttccatcttt tataattttt tgtatataac ttcttgacat atcacctaat
      841 tgttctgcta gataaatatc aagtctaact tcttcttcct cttcaagtac taaaaactct
      901 tttacttcat ccattatatc acctactttt actttcaaaa aataaaacat atatacataa
      961 aaacacagtt cctacaacta caaatacatc agctacattg aatacatatt cccatataac
     1021 tctaaagtcg aagtaatcta ccacaaaacc taatcgtact ctatcaacta gatttcccaa
     1081 tgcaccagat ataattaata taattcccaa ccttccaaat atatgtactt tccttgtatt
     1141 aagatagtat aatccaaata ccgttgcaag taatgcaaca attataaata tccattgatt
     1201 attttgtaat aatccaaatg ctgcacctct attttcgaca taagttaaat gaaatacatt
     1261 atttattatt ggtattgttg atacatccac caaattattc aatacccata ttttagacag
     1321 ttggtctaaa cctatgagta gaattattat taatatatat agcaatgatt tttcccccta
     1381 taaattattt aaaaaccaat tttatttgaa gcacactatt attattatac aaaaaaaatg
     1441 ctctaaagag caactttatt aaaattgtat tttcttaata aagctagaac aaaatttaaa
     1501 atagtactac tttttttata ttgtagattt ctacatattt aaaatcatat cattatgtat
     1561 aaaaaattta aaaataccat actaatcaat attttttgtg tattttatag attaaagatt
     1621 aatgatgtct gtaatttaaa cttacaatca ccactaatct tcaaacaaat atttttactt
     1681 ttgcacttgc ttatttttat ctaaatgttg tataagttca gatactgtta cgaatttata
     1741 tcctctctct tttaatttag gaattactac ttctaaagct tgtatagttt gtgatttatc
     1801 atttctttta gtgttataat catgtaaaag tattatatta ccattttgga cctttgtaag
     1861 gatagtattt ataattgcat ctactccagg gttagaccaa tctctagggt ctaagtttga
     1921 ccaaagaatt atattcattc ctttagaaac tataatatca cagacacttt tactcatggc
     1981 tctatatgga ggtctaaata tctttggttc tactcctatc acacttttta taatattttg
     2041 cgtatctgtt atttcttttt caacactact ataactattt tttgcaacat ttatatgtgt
     2101 aaatgtatga ttccctattt catgcccctc atcatattgt ctttttaata attctttgtt
     2161 atattctact ttttctccaa ctacaaaaaa agttgctttt acatcatttt tctttaatat
     2221 atcaagtact tgtggtgtaa aatcctcatc tggaccatca tcaaaagtca atgctaaaat
     2281 tttttcatcc tcatttcctt tttttattat tacatcttca taatcgtctt ttatattgtc
     2341 atttaaacta tatgcttctt ttgaactatc ttgattatgt gtattatgat tatatgtata
     2401 tacacctact atagataata aacatataca tataccacat attattttct tcatgtcatt
     2461 acctcttttc taattcattc tagcaatata ttttataatg tcagtttttt aatctctcaa
     2521 tattccattt ttatttaaat cagttaaatt tgttaggtct tctgaataaa agttagaatc
     2581 ttctacatca gggttttgca ttgcatcacc taataaatta ttttccttct ttgcacaatc
     2641 attacataaa cttgtatctg gcaatatatc caatctttct aaatctattt gattatgaca
     2701 taaatcacaa atcccatatg ttccattctc tattttagat aatgctaagt ctatttgata
     2761 caatcttccc tcttcatgat ttattagact gttttgcata ttttgcatat ataaatctgt
     2821 tcccatgtct cctatatgat tatcataact agataattct cctgatgaat acttttcact
     2881 tgtatgttta gttgtatctc caaataaagt attatctttc atatcctcta ctaaattagt
     2941 taaattttct ctttctttca ataatttatc tttatatttt ttagtatcca tttttaacac
     3001 ctctaatttt aatatttaca tcataaaaat agtatgtgga aaagtaaatt aaacatacaa
     3061 taaaaataag atttgattcg aaaaatggaa tgtctcaaaa tgaaattttt agttcattta
     3121 accctctttt ttatatgaaa tcaaatatat ttttatcctt tcagcaataa aaaagagact
     3181 tatctctatt ttgagatagt ctcttctttg aacataattt aaatacaatt ttttaatttt
     3241 tatagttatt attttactaa cttgccaatt tatgattatt ttgttcttta atatgttttg
     3301 cttttttaaa gaataaaaga ccaagaatta ttgctacaat aacacctatt ccattagata
     3361 tattaactgg tagtctaaat ccttcttttg cttgcattat ataactaaat gttactacag
     3421 tcataaaaag tgctggtaat gtagttatat aatggttttt atttgctttg actaaatatg
     3481 cagaaccagt ccacaacatt atcattgcaa gtgtttgatt tgcccaagag aaatatctcc
     3541 aaattatatt aaaatctata aatgtaagag caacaccaac tgcgaataat ggtaaagcta
     3601 ttaaaaatct atttttgact gcatcttgat tataattcat tgcatctgct attgtcagcc
     3661 tagcacttct aaatgctgta tctccagaag ttataggaca tgccacaact ccaagtatag
     3721 ctaaagctcc tccaataggc cccattaatc caacagatac tgtatttact actgttgctg
     3781 ctccaccatc attaaatatc acattaagtt gaggtatgcc atggaaatat gacatggctg
     3841 ctgctgccca aactaaagct attatacctt ctgcaaccat tgacccataa aatactggtc
     3901 ttgcctcact ctctttctca acacatctcg ccataagtgg tgattgagtt gcatgaaaac
     3961 ctgatattgc tccacatgct attgttataa ataagtatgg gaataaaggt gttccatttg
     4021 gattcatatt ttgtaaattt atatttggta tgttataccc ctttattatt aatcctccag
     4081 ctacacctac tgccataatt aataatgctg ctccaaataa aggatatatt ttaccaatca
     4141 atttatctat tggtaaaaca gtagccataa tataataaag tattatcact ccaacccata
     4201 tttgtgcgtt tacacctgtc aatgtcttta ataatcctgc tggacctgtt acaaatacaa
     4261 ctccaaccaa tactaaaaga ataacactaa atattctcat tgctattttt gcaccatttc
     4321 ctagataaag gcctaccaac tctgatgcag atgtaccttt gtttttcatt gatagatatc
     4381 ctgaagcaaa atcgtgtact ccaccagcaa agattgttcc aaacacaatc cataaaaatg
     4441 ctgatggtcc aaataatgct ccttgtatag ctccaaatat cggacctaac cctgctatat
     4501 ttagaaactg aattaaaaag attctcttcc atttcattgg cacataatct actccatccc
     4561 tacaagctat agctggagtt tgatttttgt cattggttcc aaaaaccttt tctacaaaag
     4621 taccatagac aaaataacct actataagta ctactattga acctaaaaag cttaccataa
     4681 attcatcccc ttagttttta tataattgct ttataaattt ttatgagttt aagaaaacta
     4741 tttccagttc aaatagtaat acttttttat gctaatataa atattaatta cattaagtgc
     4801 atatttaagt acatgaaatg actttatata tgcattaaca gcctaaattc ttttactttg
     4861 cttctactta cagttacttc ataatcacta ttttttaatt ttaatatgta tgtattatta
     4921 aaccatggta taatttcttt tactttggtt aagtttacga tataagacct atgtgttcta
     4981 aaaaatgttt tatcatttat aagattttcg acttcttata tgccttcttt taatataaac
     5041 ttttctttct cagtatatat aaatgtctgc ctttcattag cttcacagta atatatatca
     5101 tcaatatcta ttacaactaa tttatcacct ttccaaaggc taattttatg tgttatttct
     5161 tctatctcct gatttactgc ttccttctta tatttgtata aattactatt tacattaaat
     5221 agttcaatat cactcatctc acttttttct aatttattca gcatagaaat aattctctct
     5281 tcagaatatg gtttcaatat ataatcaaat acatccaaag aaaaagcatc tactgcataa
     5341 ctttcatatg ctgttataaa aacaatttta ggtcttgatt taaactgatt taatgttttt
     5401 gcaagtagca ttccatcaag atgaggtata tttatatcta aaaaaattac atctatttta
     5461 ttttcctgaa taaagtctaa cacctctata ccattggtaa attcacttac tacctcgatt
     5521 ccacttttat tttcaataaa atatctcaat tcctttcttg ctggaaattc atcttccact
     5581 attatagctc tcatacttct actcctttac ataaaatact atcaaagtcc cattatctat
     5641 tcttcttata ttaagccctc ttccatacat cagtttaagc cttaaatgta cattataaag
     5701 cccaatctta ttttctggca tattatcttg atatacatta tttataattt cctcacttat
     5761 acctatacca ttatcctcta ttgatattct cacagtattt tctaatgaat acggtaactt
     5821 ttttatggat attttcacta caccagctct accactttct aaaataccat gtattattgc
     5881 attttccact aatggttgta taatcaaact tggaatcttt atatttacat cttctatatc
     5941 ataagataca gttagtaatt ctccaaacct agctttttct atctctacaa aagatttaac
     6001 ttgttcaatc tctttattta tatcaattaa gttatcacta tattcaagat tatatcttag
     6061 ataagttgat aaatttatta ttaactctct agccttatct ggactaaatc ttataaatga
     6121 agctattgtg tttagggaat taaacaaaaa atgtggatta atttgtcttt gaagtgcttt
     6181 tatttctgct ttatttctcg cttctatatc ttttttatct ttctcaatcc cctctactat
     6241 agatataaca aatcctattc caatctgccc aactattaaa ggtaaatata ttccaccgat
     6301 tatttgaatt gctaaagaat gtggatatga tattaagtat ataagtaata tagtaaaact
     6361 ttcagaaatc atgcctacca atattccata cattactcta tgttgttttg gtatcctctt
     6421 atatagaaat cctgataata cacctgctag tatacttgat aaaagacatg gaacagatgt
     6481 tatcccccca atatccataa aatatctatg aatccctgaa aaaactcctg ctgtaatacc
     6541 taccatcgga ccaaataaaa tgccacctga tactatagat attattctag tgtttactat
     6601 tgaccccata taatttatac cactataagt tccaaatatg gctaaaaaag taaaaactaa
     6661 tgctatacag accaagtcat taaaactata ttcttccttt tgaaatatct gcttaaatat
     6721 ttttaacttt gttattagaa gaaacaatac tacaataagg cttgtctttt ctattaatgc
     6781 gattaaaatt gatgttttca taatccccct ttaaaaaata aatttttaca cactctaaat
     6841 ttattatggt tgttataaat tacatatttg aagtaatcct atcacaaaac tttccacatt
     6901 taatctttta attaaataag aaatagttgt acttctatta acaactaata ctagctagca
     6961 taagcttgta ataacttgta gttattatta actttacaat tctagtattt aattttatac
     7021 tttagaaatc tcttttctta gaaataatac tttcgaatat catataatac aatattatgt
     7081 agattcttac tcatacaaca tatttcttta aaaattttat actattcttt gttcaaatat
     7141 ctcatttgat ttaattgtgt taaacacaca ttatcatctc tctgttatat atttattata
     7201 tagccgtata tcgataaagt aaataaatcc catatttaaa ataatatgtt tgacaataaa
     7261 ttattataat gtatatttac cataat
//
LOCUS       DAMJYR010000169         7187 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.174, whole genome shotgun sequence.
ACCESSION   DAMJYR010000169 DAMJYR010000000
VERSION     DAMJYR010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7187)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7187)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7187
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.174"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(<1..520)
                     /locus_tag="PC471_003420"
     CDS             complement(<1..520)
                     /locus_tag="PC471_003420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF2790505.1"
                     /translation="MIAVKKAYKFRMYPNKKQQELINKTFGCCRFVYNKYLAKRIDVY
                     KNDKETFTYKQCSSDLTNLKKELKWLKEPDKFSLQNALKDLDNAYKKFFKEKAGFPKF
                     KSKKINRFSYKTNFTNGNIMYCGQHIKLPKLGMVKIRDKQVPKGRILNATISKEPSGR
                     YYVSLCCIDVDIE"
     gene            complement(1169..1576)
                     /locus_tag="PC471_003421"
     CDS             complement(1169..1576)
                     /locus_tag="PC471_003421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2790506.1"
                     /translation="MSKVAKATFYLMIVTIISKILGMGRELVLSSIYGTGLYTESYLT
                     AMNIPNIIFAAIGTAIVTTFIPMYQDISSKQGEKQALKFLNNVLNIIVGICIVVAILG
                     VIFSKQLVSIFAIGFEGERFLLTVKFTKILIKK"
     gene            complement(2050..2934)
                     /locus_tag="PC471_003422"
     CDS             complement(2050..2934)
                     /locus_tag="PC471_003422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HDF2790507.1"
                     /translation="MDTKKENNYENYICVGSSNVSNAMKMYLKEIEEYKMLSAGEEVE
                     LAKEIINSSSVAKEKFINSNYRLVVSIAKRYKRDSIDMLDLIQAGNIGLIKAVEKYDY
                     KKGYKFSTYATWWIKQSITRYIDDCENTIRIPIHLHQRINFVKKKKQELLNVLLREPT
                     IDEIADACGLEVDKVLELLRRDKNVVSLDTPLKEDEDSSLVEFIPSDADFKDVVIHEV
                     EQHNLKEKIEELLTGLGEQEQQVLRMRFGIDDDDPKTLEQIGKVFGVTRERIRQIEAK
                     AIRKLRHPSKLKQLKHFY"
     gene            complement(3225..3701)
                     /locus_tag="PC471_003423"
     CDS             complement(3225..3701)
                     /locus_tag="PC471_003423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction enzyme HsdR N-terminal
                     domain-containing protein"
                     /protein_id="HDF2790508.1"
                     /translation="MSKELVKDRVIKYLIEDLLVPQDMIDTNVELAEFEEGAEGILDI
                     VVNVKDEEDYYAPVMIVQCLDEDVELEGEVLQKQIEFLEEVDNITMSGRLVLTNGDAM
                     MYADWRGEEYDTEAALPTYDIMVKEFHEMEQQVKDLEEHHHHDENCGCGCNHHHEN"
     gene            3967..5142
                     /locus_tag="PC471_003424"
     CDS             3967..5142
                     /locus_tag="PC471_003424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1002 domain-containing protein"
                     /protein_id="HDF2790509.1"
                     /translation="MKFKKQISSIVVSTMLVLGSMNLSYADGTNVVTLGANLSESQKQ
                     QMLNYFGVKKDQVLVLDVTNAEERQYLQGVATEGQLGKVTISCSYVEPTKKGNGINVK
                     TANLTWVTSSMIASTLTTAGLEDANVIAAAPYPVSGTGALTGIMKAFEDASGKKLDET
                     KKEIASEELVVTGDLGDDIGQEKATGVMNDIKTEIVKNGTKDTNQIANTINNVVNNYN
                     ITITPEQKEQIKGVMKKIADQNYDYNSMKNTLDNVSDNVNEQLKKLGESVKGSGILDT
                     IGGWFSGIGDWFSGLFSGGDKKDLGILNNTNDESLGSDAVINSTEGVQINPQNNTNSN
                     NDSDTNSSKNDTSSNNENNDSSQTNENNTTNNSENQSNNNDSVGFFEKIKNWLSGLF"
     gene            complement(5223..6026)
                     /gene="proC"
                     /locus_tag="PC471_003425"
     CDS             complement(5223..6026)
                     /gene="proC"
                     /locus_tag="PC471_003425"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HDF2790510.1"
                     /translation="MKKIGFIGAGNMASAMIGGIVNSKLVEPNMVIASAYSQGTLDRI
                     DANFGINTTKDSKEVTRTSDIVIVAVKPDIYDDILEEIKDFIDDNKIIVTIAAGKSIK
                     DIESIIGEDKKIVRTMPNTPALVNEAMSSLSINKNINKEDLEAVTEVFNSFGNTEVVP
                     EYLIEAVIGASGSAPAYVFLFIEAIADAAVIAGMPRPQAYKFASQAVMGSAKMVLETG
                     KHPGELKDMVCSPGGTTIEAVKVLEEEGFRASVIKAICACIEKSKKMSE"
     gene            complement(6066..6650)
                     /locus_tag="PC471_003426"
     CDS             complement(6066..6650)
                     /locus_tag="PC471_003426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2790511.1"
                     /translation="MNKDIGAKIKQLRTQKQMTLKDMSEKTNLSIGFLSQLERGLTSV
                     ATDSLGKIASVLDVELTYFFMKPKEHKRAVLRSYEKEVFDVENSTFIHYHLSSSLKEK
                     TMLPRLIEILPSKSSEEICCYVHEGEEFVYVLEGTLTVFLGDEQIEMYPGDTIHYNSE
                     KNNHNWVNYTNKVVKILVVSIPNPFEKSDAVKEA"
BASE COUNT         2354 a         1205 c          803 g         2825 t
ORIGIN      
        1 cttcaatatc tacatcaata cagcataatg atacataata tctaccactt ggttctttag
       61 atatagtagc attaagtatt ctccctttag gtacttgttt atcccttatc ttaaccatac
      121 caagtttagg tagttttata tgttgaccac aatacataat gtttccattt gtaaagttag
      181 ttttatatga aaatctatta attttctttg atttaaattt aggaaatcct gctttttctt
      241 taaagaattt tttatatgca ttatctaaat cttttaaggc gttttgtagt gaaaatttgt
      301 caggttcttt aagccatttt aattcttttt ttaaattggt caaatcagaa ctacactgtt
      361 tataagtaaa agtttcttta tcgtttttat aaacatctat tcttttagca agatacttat
      421 tatatacaaa tctacaacat ccaaaagttt tgttaattaa ctcttgttgt tttttatttg
      481 gatacattct aaacttataa gcttttttca ctgctatcac ctcactttaa ttgttttttg
      541 atacactttt taatgatttt atcacatttt atcacattta atatattata taatgctgtg
      601 gtgaacaatt tattaatcac ctataataat gttaacaaat gtaattattt aaaacaatta
      661 tttagaaggt tttatattta attttaaata acatatttaa gaaccgtaag tcttaacttt
      721 tatcattttt gaggttgtcg ttcacataag ttcgctacta cctatgcagt tctcttatga
      781 acttcttaca ctttcatgta agcacagact atatcttatc cttcagcatt atccgttaag
      841 gtctacccac ttccactacc aatagcttgt agtgtacttc cctcaagagg aatagtcgtt
      901 gaagtttctc ctattcggag tttacctgct gattgcccat tttaaagtac ttaggattta
      961 accttatacc atctaaccaa ttttttctac tttcgtaact ttcacactta ggtttatttc
     1021 atccttatgt tttagtttga ttagctttag gggtttccag caattcaagt agtattggat
     1081 aggttctatc ctatctctac atataggttt ccctatatgc tgactatttt agctactaac
     1141 tcatgactaa agtcacgagt gtgcgtagct attttttaat caatatttta gtaaatttta
     1201 cggtaagtaa aaacctctct ccttcaaatc ctatagcaaa tatacttaca agttgctttg
     1261 agaaaataac tcctaatata gctacaacta tgcatatacc aactattata tttaaaacat
     1321 tgtttaaaaa ctttagtgct tgcttttcac cttgtttact acttatatct tgatacattg
     1381 gaataaaagt agtaactata gcagtaccta ttgctgcaaa tataatattt ggtatattca
     1441 tagctgttaa ataactctct gtatataagc ctgtaccata aattgatgat aatactaatt
     1501 ctctacccat tccaagaatc ttagagatta tagtaactat catcaaataa aaagttgctt
     1561 tagctacctt gctcatttta tcactccact tctaacatac ctctttttaa tcaagtaaaa
     1621 tttagctcat tagatataat tctatcaact aatttgcctt gcttagccct atcaaaatat
     1681 aattaacacc tatttcttaa tatatcacaa actacattat tcttcatatt tttttatttc
     1741 tccaataagt tttacatttt tcctatataa attgtatagc ttcaattata ttcaaagaaa
     1801 tggagttgct acccgataac tttaaaagta aaaaacagat tttattatct ttttaaacta
     1861 tattctaaca tattttgtcc atattgagaa attttactaa actattatgt actttattaa
     1921 ataaataaaa taacagcaca cctataaaat acaagtgtgc tgttcataaa cagattttat
     1981 aaaaaaataa ttcctaagta gagattaaca tgctttctca tactctcata tataatgtgt
     2041 ctaaaaatat taataaaaat gctttaattg ttttaactta cttggatgtc ttaattttct
     2101 tattgcttta gcttctatct gtcttattcg ttctctggta actccaaata cctttccaat
     2161 ctgttctagt gttttagggt catcatcatc aattccaaat ctcattctta aaacttgctg
     2221 ttcttgctct ccaagacctg taagtagttc ttctatcttt tcttttaaat tatgctgttc
     2281 aacttcgtgt attacaacat ctttaaaatc agcatctgat ggtataaatt caactaaact
     2341 actatcctca tcttctttta atggagtatc caaagataca acatttttat ctcttcttaa
     2401 taattccaac actttgtcaa cttctaagcc acatgcatct gcaatctcat ctatagttgg
     2461 ctctcttaat aatacattta aaagttcttg tttctttttc tttacaaaat ttattctctg
     2521 atgaagatgt ataggtatcc taatagtatt ttcgcaatcg tcgatatatc ttgttatact
     2581 ttgttttatc caccaagttg cataagtgct aaatttgtat ccttttttat aatcgtactt
     2641 ttctacagct tttataagtc ctatatttcc tgcttgaatt aaatccaaca tatcaatact
     2701 atctctttta tatctctttg ctatgctcac taccaaccta taatttgaat ttataaattt
     2761 ttcttttgct actgaagatg agttgattat ttccttagcc agctctacct cttctcctgc
     2821 tgacaacatt ttgtattcct ctatttcctt taaatacatt ttcatcgcat tcgatacatt
     2881 tgatgaaccc acacaaatat aattttcata attattttct tttttagtat ccataatcaa
     2941 accccttcta ttcatagaat ttgctaaaac aaaaataaat aatttaaacc tacataatct
     3001 acaattacat aatatcccat tctttaaatg aattgttttg tatattatgt atacaaattt
     3061 ataaaaacta tacctataat attattctac ttctaacagc aatatcctcc ttatatatat
     3121 aatttatttt attatatgtt gttatattct tagttttttg aatgatatat gtctaaaaaa
     3181 agactgttta ctaaacagtc tttttttata tatatgctca atatttaatt ttcatgatga
     3241 tgattacatc cacatccaca attttcatca tggtgatgat gttcttctaa atcttttacc
     3301 tgttgttcca tttcatgaaa ctcttttacc attatatcat aagttggaag agctgcctca
     3361 gtatcgtatt cttcccctct ccaatcagcg tacatcatag catctccatt agttaaaacc
     3421 aatctacctg acatagttat attatccact tcttctaaaa attctatttg tttttgtaaa
     3481 acttctcctt ctaactccac atcttcatct aaacattgca ctatcattac aggtgcataa
     3541 taatcttctt catctttaac atttacaaca atatctaaaa ttccttctgc accttcttca
     3601 aattctgcca actcaacatt tgtatcaatc atatcttgag gtactaataa atcttctatt
     3661 aaatatttta ttactctatc ttttactaat tcttttgaca tttatttttc tccttccgat
     3721 tctataacat tatcataatc atttctatat tatagtatac tacattaatt gttttttcaa
     3781 tctcttacaa aaattatttt ttattatctt ccttatattc ccttttttat tacaaagttg
     3841 taatcgcatt gatttaaaaa atagtttata tttagtataa tagaaatgta gcacatattt
     3901 ttattcaata tattaataaa aaaatatatt gaaaatgtaa tgtatgttaa atctaggagg
     3961 aatattatga agtttaaaaa acaaattagt tctattgttg tgtctactat gcttgtactt
     4021 gggagtatga acctttctta tgcagacggt acaaatgtag ttacacttgg agctaactta
     4081 tcagagtctc aaaagcagca aatgcttaat tactttggag taaaaaaaga ccaagtctta
     4141 gttttagatg taacaaatgc agaagaaaga cagtatttac aaggtgttgc aacagagggt
     4201 caacttggaa aagttactat ctcttgctca tacgttgaac ctactaaaaa aggaaatgga
     4261 attaatgtta aaacagcaaa cttaacttgg gtaactagtt caatgattgc ttcaaccctt
     4321 acaacagctg gtcttgaaga tgcaaatgtt attgctgctg ctccatatcc tgtaagtggt
     4381 actggagcat tgacaggaat catgaaagca tttgaggatg cttcaggaaa gaaacttgat
     4441 gaaactaaga aagaaattgc ttctgaagaa ttggtagtta caggtgactt aggagatgat
     4501 atagggcaag aaaaagcaac aggtgtaatg aatgatataa aaacagaaat tgtaaagaat
     4561 ggtacaaaag ataccaatca aatagcaaat acaataaata atgttgtaaa taattacaat
     4621 attactatta caccagaaca aaaagaacaa attaaaggtg taatgaaaaa aatagctgac
     4681 caaaattacg attacaatag catgaaaaat acactagata atgtatcaga taatgtaaat
     4741 gaacaattaa agaaattagg tgaaagtgtt aagggttctg gtatactaga tactattggt
     4801 ggatggttta gtggaatagg agactggttc tctggtttat tctcaggcgg agataaaaaa
     4861 gatttgggaa tcttaaataa tactaatgat gagtctcttg gctctgatgc tgtaataaat
     4921 tctacagaag gtgttcaaat aaatccacaa aataatacta attctaacaa tgatagtgat
     4981 acaaatagct caaaaaatga tacttcaagt aataatgaaa ataacgatag ttctcaaaca
     5041 aatgaaaaca acacaacaaa taattcagaa aatcaatcaa ataataatga ctcagttggt
     5101 ttctttgaaa aaataaaaaa ttggctttca ggcttatttt agaaattaaa caaataaaaa
     5161 gaattgccta aaatggaaat tttctataaa aggcaattct ctttatttac aataaatttt
     5221 tattactcac tcattttttt agatttttct atacatgcac atattgcttt tatcacagaa
     5281 gctctaaaac cttcttcttc aagtacttta actgcttcta tagtagttcc accaggtgaa
     5341 cataccatat cttttaattc accaggatgt tttcctgtct caagtaccat ttttgcagaa
     5401 cccataacag cctgtgatgc aaacttatat gcttgtggtc ttggcattcc tgcaattact
     5461 gctgcatctg ctatagcttc tataaataaa aatacatatg ctggtgcaga accacttgca
     5521 ccaattacag cttctataag atactctggt actacctcag tatttccaaa agaattaaat
     5581 acttctgtaa ctgcttctaa gtcttctttg tttatatttt tatttatcga aagagaagac
     5641 atggcttcat ttacaagagc tggagtattt ggcatagttc taactatttt tttatcttct
     5701 cctataatag actctatatc ttttatagat tttcctgctg ctattgtaac tataatttta
     5761 ttgtcatcta taaagtcttt tatctcttcc aaaatatcat catatatatc tggtttaaca
     5821 gctacaatta ctatatcaga ggttctagtt acttccttcg agtcttttgt agtatttatt
     5881 ccaaaatttg catctattct atcgagagtt ccttgactat atgctgatgc tataaccata
     5941 ttaggttcaa ctaattttga atttactatt ccccctatca tagcgcttgc catatttcct
     6001 gcacctatga atccaatttt tttcacttta gtacctcctt attgactaat agtaatcgtt
     6061 aaaatttaag cttctttaac tgcatcagat ttttcaaatg gatttggtat acttactacc
     6121 aaaattttca ctaccttatt agtatagttg acccaattat gattgttctt ttcactatta
     6181 tagtgtattg tgtctcctgg atacatttct atttgttcat ctcccaaaaa cactgtaaga
     6241 gtaccctcta atacatacac aaattcctca ccttcatgta cgtaacaaca aatttcctca
     6301 ctactcttac ttggaagaat ttcaattaat cttggtagca ttgttttttc ctttaaacta
     6361 gatgataaat gataatgtat aaatgttgag ttttctacat caaatacttc tttttcataa
     6421 cttcttaata cagctctttt atgctctttt ggcttcataa aaaagtaagt taattctaca
     6481 tctagtacac ttgctatttt tcctaaggaa tctgttgcca cacttgttaa tcctctttca
     6541 agttgtgata aaaaaccaat tgacaagttt gttttttcac tcatatcctt cagcgtcatt
     6601 tgcttttgag ttctgagttg ctttattttt gctcctatgt ccttattcaa aaccatcacc
     6661 cctaaataat tttaattttt tcataatata aatacaaaaa tataaatata ggtactgaac
     6721 ccatctttat ttcatttaaa aaaattttta taagtattac tataatttat caatcttttt
     6781 ttgtctacat ttttacgatt atttacgatt tatttttcac atatgtatat ttaatcttgc
     6841 caactaaaga tacatttttt ataagcattt actctttata taataaaact taagttgttc
     6901 atgtagtctt cttctggttt aattattaaa atttcaaatt atattattat ttctacacat
     6961 tattacatcc tttagtacaa ttataacata attttacaat ttaatgatat ctattcaatg
     7021 tcttttttta ctctacaaca tattttattt tagagtttaa tactatatat attttttata
     7081 tacagccttg tcatttttta gattgtcaag agttattgaa gtattttatg tagatttaga
     7141 ttttatttcc taatttctca tttaacaaga attgcttatg ataaaaa
//
LOCUS       DAMJYR010000170         7113 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.175, whole genome shotgun sequence.
ACCESSION   DAMJYR010000170 DAMJYR010000000
VERSION     DAMJYR010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7113)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7113)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7113
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.175"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(182..1021)
                     /locus_tag="PC471_003427"
     CDS             complement(182..1021)
                     /locus_tag="PC471_003427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HDF2790512.1"
                     /translation="MSDIKNEVSKKTLSKKDVIKSWLRWFFFAQSNYNYERLQSTAFS
                     HSMLPVLKKLYPDKEELKQEVETHLAFFNTEPICGCVIHGITIAMEEEKANGADEISG
                     DGMNAIKTGLMGPLAGIGDTLTQGVITPIVLAVCIGLTEGGASIAGPILFVIAQYIIM
                     TSISFGMWTNGYKYGKKAVESILQGGIVNKVIEGASILGTLVMGGLVGRFINLSTPIS
                     YTSGDFKFSLQTDLLDKIFPGLIPLVLTLLVLFALKKGLSPIKIMFILIVVGAITGIL
                     GLF"
     gene            complement(1045..1803)
                     /locus_tag="PC471_003428"
     CDS             complement(1045..1803)
                     /locus_tag="PC471_003428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDF2790513.1"
                     /translation="MHISLIQAVLIAIFYYLSWSPWLTYVGFFTWNRPLLAGFVTGII
                     LGDPVQGAIIGAGINMIYLGFISAGGAQMGDPAFAGYVGTALAIASKLDVSTAMAIAV
                     PLGTVATVLWIGKMTVNSFFAHWADREVQKGNVDRVAFINIVPPQILLFAMSFIPALL
                     VVYFGPNAIDGMLEVMNDNVLHVFNVIGAMLPALGIAMNLKLIGNKFTMPFFILGILM
                     AVYFKVDIIVISVIGVILALTITSIKYGKDSATS"
     gene            complement(1850..2323)
                     /locus_tag="PC471_003429"
     CDS             complement(1850..2323)
                     /locus_tag="PC471_003429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDF2790514.1"
                     /translation="MSNIALTRIDDRLIHGQVITAWCKITSAKRIVIVDDLVVKDPFI
                     VQVLQMAAPSTVKVEVHDVESGAEVLKSHNGDENLIVLVKYPKTVLGLVNSGVDLKEL
                     NVGGMGAGLGRKSFYKNISVSDEEKEIFRELISKDVKCFIQIVPDAKKIDVGTLL"
     gene            complement(2352..2768)
                     /locus_tag="PC471_003430"
     CDS             complement(2352..2768)
                     /locus_tag="PC471_003430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDF2790515.1"
                     /translation="MIHMAGIIIATHGNMAKAMLESAELIVGKQENVETLGLNHGDSI
                     DEFSRKLEDGIEKYKDEGVLVLLDFYGGTPFNTSAIVINKFAKVCELECLTGVNLPML
                     LELFLNRDTMKLKDLKNLCEEVGISGIKDVKTVLKL"
     gene            complement(2815..3867)
                     /locus_tag="PC471_003431"
     CDS             complement(2815..3867)
                     /locus_tag="PC471_003431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HDF2790516.1"
                     /translation="MKSVRFYGIRDTRVEDVDVPKILEKDDVIIKVKVAGICGSDISK
                     YSKTGPHMVGEILGHEFSGEVAQVGKEVRSFKIGDRVAVCPAMPCFECDECKKGLYSR
                     CNNVAIIGNKELGGCFAEYTKVKERNLIKIPDEISYETAAALEPVCIAGHGLFRSEAK
                     VGDTVVVLGTGPIGLFSIQWAKIFGSTKIIAVDVFDEKLDLAKELGADICINAKEKNI
                     VEEIKRLTDGDGADIVIESAGTPLTCGQVLLLAKKGGTVLYAGVPYGDVALTREQFEK
                     IVRSELTVKGTWFGNSFPFPGKEWSAGLYHMQKGDMNVEKLVTHRINLEEVPAYFEKV
                     YKRDIFFGKIMINIDN"
     gene            4263..4943
                     /locus_tag="PC471_003432"
     CDS             4263..4943
                     /locus_tag="PC471_003432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HDF2790517.1"
                     /translation="MKKVYQGKTKDVFELDNGNYLLKFKDDVTGKDGVFDPGENSVGL
                     SIDGIGRANLETSVKFFEILNNAGIKTHYVSANLEEATMEVLPAKVFGNGLEVICRYR
                     AVGSFLRRYGSCVEEGAKLDCYVEATLKDDDRCDPLITSEGLEALNIMTQAQFDSMKS
                     MTQKISNIVRDTIAEKGLELYDIKFEFGFYNDEVILIDEIASGNMRVYKDGVIVDPMD
                     LTALLLDK"
     gene            complement(5076..5396)
                     /gene="sugE"
                     /locus_tag="PC471_003433"
     CDS             complement(5076..5396)
                     /gene="sugE"
                     /locus_tag="PC471_003433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000118520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HDF2790518.1"
                     /translation="MKWLFLFVAGLFEVFWAFQLKNSHGFSKLTPSILTVVGMIVSFY
                     FLSLALKHLPLGTSYAIWTGIGTVGTAIVGMAILNEPISISRIACIGFIVVGIVGLKL
                     LSTN"
     gene            5778..6605
                     /locus_tag="PC471_003434"
     CDS             5778..6605
                     /locus_tag="PC471_003434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HDF2790519.1"
                     /translation="MNFYKIAVCQMITTENKIENINHAVDMVTEAAINGAKIVVLPEM
                     FNCPYENKYFPKFAEEYPGETTTILSKLAEKHGIYLVSGSIPELEDGKIYNTCYVFDK
                     NGALIGKHRKMHLFDIEVTGKVSFKESDTLTAGNDVTVIDTEYGKVGIAICYDIRFPE
                     LSRLMALKGAEIVILPAAFNMTTGPAHWELSIRMRALDNQIFYVGAAPARNMNASYIA
                     FGNSRISDPWGRIIAQADEKECIIYADIDRDLIPDIRQQLPLLKHRRTDLYELNTLK"
BASE COUNT         2411 a         1292 c          839 g         2571 t
ORIGIN      
        1 attaaaaatc tttctgagat actttttaaa atattaaata ctacatatag cttacataag
       61 tttaaaattt aatctattat aaaattaccc taattgccaa actttttaat ataaattttc
      121 caacatccta caaatataat caatagatat agaatcctaa cttacaccaa tatatattta
      181 tttaaaatag tcctaaaata cctgtaatag ctcccactac tatcagtata aacattattt
      241 ttattggaga aagacctttt tttaatgcaa agagtactaa taaagtaagt accaaaggaa
      301 ttaatcctgg aaatatctta tcaagtaaat ccgtttgaag actaaattta aaatcaccac
      361 tagtataact tattggtgta gataaattga taaacctacc tacaagcccc cccatgacta
      421 aggtacctag tatagaagcc ccttctataa ccttatttac tattccacct tgaagtatac
      481 tttcaacagc ttttttccca tacttatatc catttgtcca cattccaaaa gaaattgaag
      541 tcattattat atattgagct attacaaata atataggtcc tgcaattgat gctcctccct
      601 cagttaaacc aatacaaaca gctaacacta taggagttat aactccttga gttaaagtat
      661 ctccaattcc cgctagtgga cccatcaagc ctgttttgat agcattcata ccatctccac
      721 ttatttcatc tgctccatta gctttttctt cttccatagc tattgttatt ccatgtataa
      781 cacatccaca tataggttct gtgttaaaaa atgctagatg agtctccact tcttgtttta
      841 actcttcctt atctggatat aattttttaa gtactggcaa cattgaatgt gaaaaagctg
      901 ttgattgcaa tctttcataa ttgtaattag attgtgcaaa gaaaaaccat cttaaccatg
      961 attttattac atctttcttg cttaacgttt tcttagatac ttcatttttt atatctgaca
     1021 taatattttc cccctcacat gtatttaaga tgtagcacta tcttttccgt acttaattga
     1081 agtaattgta agagcaagta ttactccaat aacagatatt actattatat ctactttaaa
     1141 ataaacagcc attagtattc ctaatatgaa aaagggcata gtaaacttat ttccaatcaa
     1201 ttttaaattc atagcaatcc caagtgctgg caacatagcc ccaattacat taaatacatg
     1261 taacacatta tcattcatta cttccaacat accatctatc gcatttggac caaaataaac
     1321 aactaataat gctggtataa aactcattgc aaataacagt atctgtggtg gtactatatt
     1381 tataaatgct actctatcaa cattcccttt ttgaacctct ctatcagccc aatgtgcaaa
     1441 aaaagaattt actgtcatct ttccaatcca aagtacagtt gcaacagttc ccaatggtac
     1501 agctattgcc attgcagtac ttacatctag cttagatgct atagctaaag cagtacctac
     1561 atatcctgca aaagctgggt cccccatctg agctccccct gctgatataa aacctagata
     1621 tatcatattt atacctgctc ctataattgc tccttgaaca gggtctccta atataattcc
     1681 cgttacaaat ccagccaata gaggtctatt ccatgtaaaa aatccaacat aagtcaacca
     1741 cggactccaa gaaagataat aaaaaattgc tattaatact gcttgaatta gtgaaatgtg
     1801 catattcttc ctccctattt tcttttaaag atttacaaga tagtcttctt tacaataatg
     1861 ttcctacatc aattttcttt gcatctggta ctatttgtat aaagcattta acatcttttg
     1921 atattagctc tctaaatatt tctttttctt catctgacac agatatattt ttataaaaac
     1981 tttttctacc aagccctgct cccatacctc caacattcaa ttcctttaaa tctaccccac
     2041 tatttactaa acctaagaca gttttagggt atttgaccaa tactattaaa ttttcatctc
     2101 cattatgtga ttttaagact tcagcacctg attctacatc atgtacttca accttaacag
     2161 ttgatggagc tgccatttgt aaaacttgaa ctataaatgg gtcctttact actaaatcat
     2221 ccacgattac tatccttttt gctgatgtaa tcttacacca agcagtaata acttgcccat
     2281 gtattaatct atcatcaatt cttgttaatg ctatattact cataattatt cctccagatt
     2341 tttaatttat tttacaactt taaaacagtt ttaacatctt tgattcctga tatcccaact
     2401 tcctcacata aattcttcaa gtcttttaac ttcatagtat ctctgtttaa aaataactct
     2461 aaaagcatag gtaaatttac acctgtcaaa cattctaact cacatacctt agcaaattta
     2521 tttattacaa tagcagatgt attaaatgga gttccgccat aaaaatcaag caatactaaa
     2581 accccttcat ccttatattt ctcaattcca tcttctaact ttctactaaa ctcatctatg
     2641 ctgtcaccat ggtttaatcc taaagtttct acattttcct gtttgcctac tattaattca
     2701 gcactttcta acattgcttt agccatattt ccatgtgtgg ctataattat tcctgccata
     2761 tgaatcatac ctttcttaac cttttttata tggattatta tttagtactc taatctaatt
     2821 atctatatta atcattatct taccaaagaa aatatccctt ttgtatactt tttcaaaata
     2881 agctggtact tcttctaaat ttattctatg tgttactaat ttttcaacat tcatatcacc
     2941 cttttgcata tgatacaatc cagcactcca ttcttttcct gggaatggaa aggagtttcc
     3001 aaaccaagtt ccctttacag ttaattcact tcttactatc ttttcaaatt gctctctagt
     3061 caaagctaca tccccatatg gtacacctgc atacaatact gtacctccct ttttagctag
     3121 caacagtact tgaccacatg tcagtggagt accagccgat tcaataacaa tatctgcacc
     3181 atctccatca gttaaccttt ttatttcttc tacaatattc ttttcttttg catttataca
     3241 aatatctgcc cctaactctt ttgccaagtc taacttctca tcaaatacat ccacagctat
     3301 tatttttgta gaaccaaata tttttgccca ttgtattgaa aataatccta ttggacctgt
     3361 accaagtact acaacagtat ctccaacttt agcttctgac ctaaaaagac catgtcctgc
     3421 tatacatact ggttctagcg cagcggcagt ctcataactt atctcatctg gtatttttat
     3481 taaatttctc tctttcacct ttgtatactc tgcaaaacaa cctccaagtt ctttatttcc
     3541 tattattgct acattattac atcttgaata caagcctttt ttacattcat cacattcaaa
     3601 acatggcata gctggacaaa cagcaactct atctcctatt ttaaaactcc ttacttcttt
     3661 accaacctgt gccacttctc cagaaaattc atgccctaaa atttctccaa ccatatgtgg
     3721 acctgtcttt gaatattttg atatatcaga accacatatt ccagctactt ttactttaat
     3781 tattacatca tctttttcta atatttttgg tacatccaca tcctccactc ttgtgtctct
     3841 tataccatag aatctaactg acttcactat taaccacccc tactttttat ttgagaatat
     3901 atccctattg tttatatgtt aaatatctaa agcaactttc atgccaatat atgtaagcat
     3961 tgtattcatg cattttataa aattatcatt aaatgtgtgt caaagtgtgt caaaagtttc
     4021 ttatattaaa acttatgtgt gtcaatataa acgacactaa cattttatat tagttctctt
     4081 attataagtt ttataaaatg tacttttttt gttattttta taaaattgtt cggaaatttc
     4141 ccttctattt gtgatataat attaaatata aggtatcaat ataaattttc atgcgaatat
     4201 cagaactttt ttgacactaa tgccttattc aatataataa tttaactttt tggaggttgt
     4261 ttatgaaaaa agtgtatcaa ggaaagacta aagatgtttt tgaattagat aatggaaatt
     4321 acttacttaa atttaaagac gatgtaactg gtaaagatgg agtatttgac ccaggcgaaa
     4381 atagtgtagg tctaagtata gatggaattg gtagagctaa tttagagaca tctgttaagt
     4441 tctttgaaat tctaaacaat gctggaatta aaactcatta tgtaagtgct aatttagaag
     4501 aagcaactat ggaagttcta ccagcaaaag tttttggtaa tggattagaa gttatttgtc
     4561 gttatagagc tgtaggtagt ttcttacgtc gttatggttc atgtgttgaa gaaggtgcta
     4621 aactagattg ttacgtagaa gcaactttaa aagatgatga ccgttgtgac cctcttataa
     4681 catctgaagg tttagaagct ttaaacatta tgactcaagc tcaatttgat tcaatgaaaa
     4741 gcatgactca aaaaatttct aacatagttc gtgatactat agctgaaaaa ggtcttgagc
     4801 tttatgatat taaatttgaa tttggattct acaatgatga agtaatctta atagatgaaa
     4861 tagcatctgg aaatatgcgt gtttataaag atggtgtaat agttgaccct atggatttaa
     4921 ctgctctttt attagataaa tagtatatac atataattta caaatataat aaacttataa
     4981 ataagataag ctatgaagta aagacttttt aaatgttgat tatttttaaa cattttctcg
     5041 cctttaattt catagcttat ttaaattaat atactctaat tagtagataa tagctttaac
     5101 cctacaattc ccaccacaat aaaacctata catgcaattc ttgatatact aataggctca
     5161 tttaaaatag ccatgcctac aatagctgta ccgacggtac caattccagt ccatattgca
     5221 tatgaagttc caagtggaag atgttttagt gctaatgaca aaaaataaaa acttacaatc
     5281 attccaacaa cagtaagtat acttggagtt aatttagaaa atccatgtga attttttagt
     5341 tgaaaagccc aaaacacttc aaacaaacct gcaacaaaca aaaataacca tttcataaat
     5401 tcatccctct tttcaaatat gtctcttatt tttccaataa aataacctga gaaatattat
     5461 aaaatatctc ccaggttttt gtccttccgt gttcacaaca aaaactatgt tgtgggtttt
     5521 atctcggacc agaccaatca ttaataattg cggaacccta taaaactcta ttaattattc
     5581 tattttatct ataataatac tatcaacata tataaaaaaa gtcaatatat gggttccata
     5641 tataataaaa ataactttta tatacctttt aatattctta ttcacttctc tccataccca
     5701 tttgggtcaa tctaagaata tctaattttt atttaatatg ttataataac ataaaaatat
     5761 tgaaaggagt aatttctatg aatttctata aaattgcagt atgtcagatg attactacag
     5821 aaaataaaat tgaaaatatc aatcatgctg tagatatggt aacagaagct gccatcaatg
     5881 gagctaaaat tgtagttttg ccagaaatgt ttaactgccc atatgaaaat aaatattttc
     5941 ctaaatttgc tgaagaatat ccaggtgaaa caacaacgat attaagtaaa ttagctgaga
     6001 aacatggaat ttatctagtt tctggtagta ttcctgagtt agaagatgga aaaatctata
     6061 atacttgcta tgtatttgat aaaaatggag ctctaattgg aaaacatcgt aaaatgcact
     6121 tatttgacat tgaagttaca ggaaaagtta gttttaaaga atcagatact cttacagctg
     6181 gaaatgatgt cactgtaata gataccgaat atggaaaagt tggtatagct atttgctatg
     6241 atattcgttt ccctgagtta tcacgtttaa tggctcttaa aggtgctgaa atcgttatct
     6301 tacctgctgc atttaatatg actactggtc ccgcccattg ggagttatct ataagaatgc
     6361 gtgctttaga taatcagata ttctatgttg gtgctgcacc tgctagaaat atgaatgctt
     6421 cttatatagc ttttggaaat tcaagaattt cagacccttg gggaagaata attgctcaag
     6481 ctgatgaaaa agaatgtatt atttatgctg acattgatag agatttaatt cctgacataa
     6541 gacaacagtt accacttctt aagcacagaa gaactgattt atatgaactt aatactttga
     6601 aataaaacag aacataaccc cataaatcat aaattaagtg ttaaaaaaat ttatggggtt
     6661 ctcattaatt tgatttaaaa ttaatttcat aaaaataatg ctaatattaa tttaattaaa
     6721 taatactaat aattactaat gattatgcta attatcagca tttactaatt taatcactat
     6781 tagttatttc ttttattaaa tatctttact tttttactta ttatgtcttc tagctttaca
     6841 tttggattat ggtatttctt tctattttta gtcacatctt tctattgaat tacttgctta
     6901 aaacaccaat gcacacatgc cagataatgt tcacaattat ttagaaatct tgctctacct
     6961 tcttcaatgt tgatattatc tctcctagtt gatattatct ttcctaaaat ttcttttaat
     7021 taaaaaacaa cattagttga tatatcaagt atattttaag caactgaaaa aaacaataat
     7081 aattgatata tcaactaatt aatgtagatt aat
//
LOCUS       DAMJYR010000171         6990 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.176, whole genome shotgun sequence.
ACCESSION   DAMJYR010000171 DAMJYR010000000
VERSION     DAMJYR010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6990)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6990)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6990
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.176"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(134..1312)
                     /locus_tag="PC471_003435"
     CDS             complement(134..1312)
                     /locus_tag="PC471_003435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2790520.1"
                     /translation="MQELKPVARISKSKLKDILDIFKTSIRKVEKIDYSACNSCAIKN
                     NPSFDSNKLNEFCSNCPKLKTISEFSYVAEKETELGMIEIVTNESKCMALSKSAIKQY
                     LAYHFIVSNKNYVRKAVSFQNIADICNVSIVTARHNHNVLLRVGLVYSTEIKRGKFDI
                     VIDEEYKNHLKKEDGGKGYLTMSLDTLNHLLSFENVNELKLEIKKLIWADAKGSKLGK
                     RIRFKKDNLISILPEYIKKSNKRTEAFLRSSKTLFDVSNDTIDTKRYETKETISEKYT
                     EPIRIQISKFFDNTGCSLSSNYSKVLSQINLDRYNLFEEDLRSKLDMEKDIIINDLVG
                     LSVQYGVTRVLAALDYMFNKYTVDEEDINYNEIRNPGAFIRTLIVQSINKNGSLAIAI
                     "
     gene            complement(1329..1505)
                     /locus_tag="PC471_003436"
     CDS             complement(1329..1505)
                     /locus_tag="PC471_003436"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2790521.1"
                     /translation="MLENLIKEYFLDSFCIENINTDMKNLEIKIDNIYNCLNNTLNEN
                     QRKLLSQFDELLKI"
     gene            complement(1579..1842)
                     /locus_tag="PC471_003437"
     CDS             complement(1579..1842)
                     /locus_tag="PC471_003437"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF2790522.1"
                     /translation="MSIKYNNEKVFLVSENDKEKLEKAGINFVSLDGNYYVVSQGKKP
                     KKFTDNEIREIKNDLDNGLSIRKCAERWDCSTNVIMKIKQNTY"
     gene            complement(1929..2192)
                     /locus_tag="PC471_003438"
     CDS             complement(1929..2192)
                     /locus_tag="PC471_003438"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain containing protein"
                     /protein_id="HDF2790523.1"
                     /translation="MSIKYKDKVVFIVDSSEKEKLDKSGIAYETLENENYYVVQQGRR
                     SKRFNDEMQNQIRKELENSTIRAIAKKYKTSTGIIQSIKDFKY"
     gene            complement(2222..2608)
                     /locus_tag="PC471_003439"
     CDS             complement(2222..2608)
                     /locus_tag="PC471_003439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012058279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="retropepsin-like domain-containing protein"
                     /protein_id="HDF2790524.1"
                     /translation="MKNFKYKNGLLYTDIEILIENKNILVKDTIIDTGASHTIISSSY
                     LEESDIGFNDDDTIVKASGYGGTVQYSVRKLVNKVACGDITLENIKLDFGEIDPEEKV
                     NGLLGLDFLRNANLIIDLVDNLLIKK"
     gene            complement(2610..2855)
                     /locus_tag="PC471_003440"
     CDS             complement(2610..2855)
                     /locus_tag="PC471_003440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012058278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2790525.1"
                     /translation="MKWSEVRNLYPNSFVKLEILESHIQNEKEYIDEVALIKVIPNNK
                     EALKEFNRCKDKEIVYNTKNKEFVIDIIKHIGIRRGL"
     gene            complement(2924..3190)
                     /locus_tag="PC471_003441"
     CDS             complement(2924..3190)
                     /locus_tag="PC471_003441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain containing protein"
                     /protein_id="HDF2790526.1"
                     /translation="MSLKYKDKEFVLVEKKNINELDNSNINYIDLKDKQYYVVTQGRR
                     SKRFNNEDVSEIKKDLDNGMSLRKCAEKWNCSTRTIQDIKQNKY"
     gene            complement(3664..5232)
                     /locus_tag="PC471_003442"
     CDS             complement(3664..5232)
                     /locus_tag="PC471_003442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2790527.1"
                     /translation="MSKDVKENISNEQKDLNKINLIYHIIYDNDRFKMITRKYFEIFR
                     LKGMSAEEISLKFEEFLYSLISDKFKKINTVNDVKKLRNQENSSSLKLKKRWSDMTLD
                     SLSEDTKSSRKKSSKYLKKELEIVESEYLKNGRIISAGVKNPKELKEQRKDFLDEWKS
                     SSSYNICDYPYLQELSTSKINSAFIHDILMCITEVLKDDYDFDINKIAIKTPPHISPG
                     IFLPFTKGKRYSHLNVKKNENEYMSDDYSAKNLSGKQMNFFYKFSIGQDENIENLKLE
                     LINPDTEKRALPKIYLDKQDLEIIRFVYTYSHMNSFSFYLGDLVKYLGLSDGKKNYIN
                     IKNRLLKLPYYTFYSNQTNDKGELKYEISFNLFSSTTIVNDENNNNRELVKITKSFIE
                     EVERVNTDIMYRNELEKLQLTQSINLAYFLEGRRSFLISCGEDIKNKSFRYDIEDLKF
                     DVHLNKSKTVKQNMEYLELGFNEIKENQFIIKDYKRGNSYFDVYFYEDFEKRKRLINN
                     TILSLPDYMIENKS"
     gene            complement(5237..6163)
                     /locus_tag="PC471_003443"
     CDS             complement(5237..6163)
                     /locus_tag="PC471_003443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2790528.1"
                     /translation="MNYNLKNENVLSELLQNEDEKTRIGILKVYKVLIFWFAKEGKLV
                     EEENDYIVEIVGNDKEEIVRDMSQNKTYNAIMAIIFKINKIFKTLNYDIHFSKPKNLV
                     AEFDLSKYLTQDEVLQVCSKLTNAQDKFIIYAAFCGLIGKDAEDLRGIKIKDIDLEKR
                     LIKLKDTEFKLDKFLFDFTKDTLEQKEYKQYLEQDSNAAEFYLFNSNSEYLIKTKPDA
                     RYGNGLKEMGYSTIRKRLLIINDYLDNKVQLNFTNLKISGIMHQMHLNEKKYTQSGYW
                     TNKSLKEFKEDNNLNFKIQEVLSIYKQKYIKE"
     gene            complement(6296..6868)
                     /locus_tag="PC471_003444"
     CDS             complement(6296..6868)
                     /locus_tag="PC471_003444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HDF2790529.1"
                     /translation="MTWNDLKNNDITYIVEYINNKLNNSKSLTKVAIGLGVSESSIRK
                     YLTKRGYKRINDEYVFIGDNSMTKVVNNRQQSNNDNTFNMTIEDDNNHNIVIDNQFKN
                     NIVSLAKDYDKIQDVLNWFENKEDTNVIEVVQDGIKIDLHSKDAIRTTVRLNKDAWNL
                     FDEFCGKFREFNKSDLMSMALLEYIKKYDK"
BASE COUNT         2255 a         1075 c          671 g         2989 t
ORIGIN      
        1 attaaaatag gcataaaaaa atagagctaa tagagatttc acgctcttta tagctctatt
       61 taagtatttt ataatttgaa tacaaacact aataggagta gccaaaatca ttttttttga
      121 actactccat atattaaatt gctattgcta aacttccatt tttatttatg ctctgaacaa
      181 tcaatgttct tataaatgca ccaggattac gtatttcatt ataatttata tcttcttcat
      241 caactgtata tttattaaac atataatcta atgctgctaa aactctagta actccatact
      301 gcaccgacaa tccaactaag tcatttataa taatatcttt ttccatgtct aatttacttc
      361 ttaaatcttc ttcaaataaa ttatatctat ctaaatttat ctgagataac actttactat
      421 aattagaact caaagagcaa ccagtattat caaagaattt cgatatttgt attctaattg
      481 gttctgtata tttctctgag atagtttctt tcgtctcgta acgctttgta tctattgtgt
      541 cattacttac atcaaataaa gttttagaac ttcttaaaaa ggcttctgta cgcttgtttg
      601 atttctttat gtattctgga agtatagaaa taagattgtc ttttttaaat cgtattcttt
      661 tacctaactt acttccttta gcatcagccc aaattaattt tttaatttca agttttaact
      721 catttacatt ttcaaaactt aataagtgat ttaaagtatc taatgacata gttaagtaac
      781 cttttccacc atcttctttt ttaagatggt ttttgtactc ttcatctatg actatatcaa
      841 atttacctct ttttatttct gtagaataaa ctaatccaac tcgtagtagt acattatggt
      901 tatgtctggc tgtaacaatg gaaacattgc atatatcggc tatattttgg aaggatactg
      961 cttttctaac ataattttta ttagatacta taaaatgata tgctaaatac tgttttatag
     1021 cacttttact taaagccata catttacttt cattagttac aatctctatc atacctaatt
     1081 ctgtttcttt ttctgcaaca taagaaaact ctgatatagt ttttaattta ggacaatttg
     1141 aacaaaattc atttaattta ttactatcaa aagaaggatt attttttatt gcacaagaat
     1201 tacaagcaga ataatctatt ttttctactt ttcttataga agttttaaaa atatctaaga
     1261 tatcttttaa ttttgatttt gatattctag caactggttt taattcttgc atatttaatc
     1321 ctccttttct aaattttaag taattcatca aattgagata atagtttcct ctgattttca
     1381 tttaaagtat tatttaagca attatatata ttgtctattt ttatttctaa attcttcata
     1441 tctgtattaa tattttcaat acaaaaggaa tctaaaaagt attcttttat caaattttct
     1501 aacatctaaa atcaccccta tataatctca taggtatatt ataacatata attgtgtttt
     1561 tattaagtaa aaacacaatt agtaagtgtt ttgctttatt ttcatgatta catttgtact
     1621 acagtcccat ctttcagcac actttcttat gctcaatcca ttgtctaaat catttttaat
     1681 ttctcttatt tcattatctg taaacttttt aggtttctta ccttgagaaa ccacataata
     1741 atttccatct aaactcacaa agtttatacc agctttttct aatttttctt tatcattttc
     1801 acttaccaaa aatacttttt cattattata tttaatactc ataattcgct ccttggtatt
     1861 aaaagttata atattcctat aaacttatta tactaatttt gtttcttgtt gtaaatgaag
     1921 aaacaaaatt agtatttaaa atcttttata ctttgtatta tccctgtgct tgtcttatat
     1981 tttttagcaa ttgccctaat agtactattt tctaattctt ttctaatttg attttgcatt
     2041 tcatcattaa atcttttact tcttcttccc tgttggacta cataataatt ttcattttct
     2101 aaagtttcat atgcaattcc tgacttatct agcttctctt tctccgaact atctacaata
     2161 aagactactt tatctttata tttaatactc atattgtgat aactccttat ataaacatgt
     2221 tttatttttt tatcaataga ttatctacta aatcaataat caaattagca tttcttaaaa
     2281 aatctaatcc taataaacca tttacttttt cttctgggtc aatctctcca aaatctaatt
     2341 ttatattttc aagagtgatg tcaccacaag caaccttatt aactaatttc cttactgaat
     2401 attgaacagt tccaccataa ccagaagcct ttacaattgt atcatcatca ttaaatccaa
     2461 tatctgattc ttctaaataa gaagatgata ttatagtgtg agatgcccca gtatcaatga
     2521 ttgtatcttt tacaaggata tttttatttt caattaagat ttctatatct gtatataata
     2581 atccattttt atatttgaaa tttttcatac tacaaacctc ttcttatacc aatatgtttt
     2641 atgatatcta ttacaaactc tttattcttg gtgttatata caatttcttt atctttacat
     2701 ctgttgaatt cttttaaagc ttctttattg ttaggaatga cctttataag agctacttca
     2761 tctatatact ccttttcatt ttgtatatgt gattccaaaa tttctaattt tacaaatgaa
     2821 ttaggatata aatttctaac ctctgaccat ttcataggaa tcaactcctt tatagtataa
     2881 gaatattata atattaatga ggtaaaatgt aaataatacc tcgttagtac ttattttgtt
     2941 ttatatcttg tatggttctt gtactacaat tccatttttc agcacatttt cttaaactca
     3001 taccattatc taagtctttt tttatttcac ttacatcttc attattaaat ctcttacttc
     3061 ttctaccttg tgtaactaca taatactgct tgtcctttag gtcgatatag tttatattag
     3121 agttatctaa ttcatttata ttcttttttt ctacaagtac aaattcttta tctttatatt
     3181 taagactcat aattttctcc tttgaaaaat caataatttc agaagtacgg agctgatata
     3241 gccattttgg tgatgattcc ttttactcag atttatctta cccaaaaaat aaatttatgt
     3301 ctatatttgt taagtacgga gctgatacgg tcatttagct caaatagagt ggtttcaacg
     3361 tcttctgtta agtacggagc tgatacggtc atttagctca gacatagtga tttcagaaga
     3421 cgtgagattt gaaattacta agaaaagtaa aaaatatgtt acaaaaaaaa tttttcgaca
     3481 aggaaataat taaaaaaagg tctatttttt gttaagtacg gaagatatac ggtcatttag
     3541 tttttttgaa aaatctattt tttgttaagt acggagaata tacggtcatt ttactttttt
     3601 tattaagttt atttaaaata aattcgacat tttttgttaa gtacggagaa tatatggcca
     3661 tttttaagat ttattttcta tcatgtagtc aggtaaagaa agtatagtat tatttataag
     3721 acgttttctt ttttcaaaat cttcatagaa ataaacatca aaataagaat ttcctctttt
     3781 atagtcttta attataaatt gattttcttt tatctcatta aaacctagtt ctaaatactc
     3841 catattttgc tttactgttt tacttttatt aagatgaaca tcaaatttta aatcttctat
     3901 gtcatatcta aaacttttat tttttatatc ctcaccacaa gatataagaa aagaccttct
     3961 accctctaag aaataagcta gattaattga ctgtgttaat tgtaattttt caagttcatt
     4021 tctatacatt atatctgtat taactctttc gacttcttca ataaaagatt ttgtgatttt
     4081 aaccaactct ctattattat tgttttcatc atttactatt gttgtactag aaaacaaatt
     4141 aaaactaatc tcatacttta attcgccttt atcattagtt tgattcgaat aaaaagtata
     4201 ataaggtaat tttaagagtc tatttttgat attaatatag ttcttttttc catctgaaag
     4261 tcctaagtac tttactaaat cgcctaaata aaatgaaaaa gagttcatgt ggctataagt
     4321 ataaacaaat ctaataattt caaggtcttg tttatctaaa tatatttttg gcaaagccct
     4381 tttttctgta tcaggattta ttagctctag tttaagattt tcaatatttt catcttgccc
     4441 aattgaaaac ttatagaaaa aattcatttg tttacctgaa agattcttag cactatagtc
     4501 gtctgacatg tattcatttt catttttttt aacattcaag tgcgagtatc ttttaccttt
     4561 tgtgaaaggt aaaaaaattc caggtgaaat atgtggtgga gttttgattg caattttatt
     4621 aatatcaaaa tcataatcat cttttagtac ttctgttata cacattagta tatcatgtat
     4681 aaatgcggaa tttattttgc tagtacttaa ctcttgtaaa tatgggtaat cacatatgtt
     4741 ataactagag cttgacttcc actcatctaa aaaatctttt ctttgctcct ttaattcttt
     4801 tggatttttt actccagcac taatgattct tccatttttt aaatattcac tttcaacaat
     4861 ttctaattct ttttttaggt atttactgct tttttttctt gaagatttag tatcttcact
     4921 tagagaatct aaagtcatat cagaccatct cttcttaagt tttaatgatg atgagttctc
     4981 ttgatttctt aattttttaa catcatttac tgtatttatc tttttaaact tatctgaaat
     5041 aagagaatat agaaattctt caaattttaa acttatttct tcagcagaca taccctttaa
     5101 tctaaaaatt tcaaaatatt ttcttgttat cattttaaat ctatcattgt catatataat
     5161 atgatagatt aaatttattt tatttaaatc tttttgttca ttacttatgt tttctttaac
     5221 atctttactc ataaggttac tccttaatat atttttgctt ataaatagat agaacttctt
     5281 gtattttgaa gtttaaattg ttatcctcct taaactcttt caagcttttg ttagtccaat
     5341 atccagattg agtatatttt ttttcattta gatgcatttg atgcataata ccagaaattt
     5401 ttaaattggt gaaattcaac tgtactttat tatctaaata atcattaata ataagtaatc
     5461 ttttgcgaat tgttgagtat cccatctctt ttaatccatt tccatatcta gcatctggtt
     5521 tagttttaat taaatattca gaattagaat taaatagata aaattcagca gcattgctgt
     5581 cttgctccaa atattgttta tactcttttt gttctaatgt atcttttgta aaatcaaata
     5641 agaacttgtc cagcttaaat tctgtatcct ttaactttat taatcttttt tctaaatcta
     5701 tgtcttttat ttttataccc cttaggtctt cagcatcttt accaataaga ccacaaaaag
     5761 ctgcataaat tataaactta tcttgtgcat tagttagctt actacatacc tgaagtactt
     5821 catcttgtgt tagatatttt gataaatcaa attcagcgac taaatttttt ggtttagaaa
     5881 agtgaatatc gtaatttaaa gttttaaata tcttattaat tttaaaaatt atagccatta
     5941 tggcattgta tgttttattc tggctcatat ctctcacaat ttcttcttta tcattaccta
     6001 ctatctcaac aatatagtca ttttcttcct caacaagctt tccttcttta gcaaaccaaa
     6061 atattaacac tttataaact ttgagtatac caatccttgt tttttcatcc tcattttgta
     6121 atagctctga taatacattt tcatttttta aattataatt cataaattca aacatccttt
     6181 ttttaattat atatatatta taacatttac cttctccgtt ttaaatagaa aataaaaaaa
     6241 ataacctcaa aataatgagg ttatttaaat taaaactacg attttattgt catttttatt
     6301 tatcatactt ctttatgtat tctaataaag ccatactcat taaatcagac ttattaaact
     6361 ctctgaattt tccacaaaac tcatcaaata aattccatgc atctttattc aacctaactg
     6421 tagtccttat agcatctttt gaatgtaaat caatcttaat accatcctgt acaacttcta
     6481 ttacatttgt atcttcttta ttctcaaacc aatttaatac atcttgtatt ttatcatagt
     6541 cttttgctaa agagactata ttatttttaa attggttgtc aattactatg ttgtgattgt
     6601 tgtcatcttc tattgtcata ttaaatgtat tatcattatt actctgttgt ctattgttta
     6661 ctacttttgt catactattg tcaccaataa acacatactc atcatttata cgtttataac
     6721 ctcttttagt aaggtatttt cttatgctac tttcagacac accaagccct atagcaactt
     6781 tagtcaaact cttactatta tttaacttat tattaatata ttcaactata tatgttatat
     6841 cattgttttt taaatcattc caagtcatat actcacctct aaattaagta tactacatga
     6901 gattattgtt gtcataccat attaaccagt tgactaatag tttttattta catatatttc
     6961 taaagtgcaa caaactttga tataatttat
//
LOCUS       DAMJYR010000172         6951 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.177, whole genome shotgun sequence.
ACCESSION   DAMJYR010000172 DAMJYR010000000
VERSION     DAMJYR010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6951)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6951)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6951
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.177"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            16..2193
                     /locus_tag="PC471_003445"
     CDS             16..2193
                     /locus_tag="PC471_003445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HDF2790530.1"
                     /translation="MYNKYNNLYLLLGKENVNMDSYKQLKAKIPCKETGIEIKRTLCD
                     ICSPGVHCGIDAYVKDGKVIKIEGTKEHPMNKGLLCTKGLCNREYIYREDRIKTPLKR
                     IGKRGEGKFKPISWDEAYREIAEKLNRVKSEYGAKHVAFFTGYGKWYRPFLQRFASSF
                     GSPNYGTESSSCSTSAQMAGNVATGRNFTMQDMENAKTYLGWGFNPYYSKFLNSVNLI
                     KLKEKGLKFIIVDPRITPTTQKLADLHLRIKPGTDGALALGMANLFIQKDLIDREYIE
                     KHVHGFNEYKEYVKLFDLDTVSRITGVSKDNIVKAVEMLSQGPFCINESAAPIVHHQN
                     GFQNYRAMMSLSAITGNYDRIGGNIPKQDYFAGKIGGFQTLEKEFVKEVLPKNINERI
                     GGERFPLWNEMVTQFQAMDLPRQIEEKTPYEIRALFCHGINARMFPNSGRLFKALNSV
                     DFFVNIDLFMTDTSKYADILLPACTSFEREECKSYPGGYITYTKKVIDKLYDSKSDVE
                     ILSDLANAMNIDDDLLKAGYEASVRHMFKNTCVDVDKLKESDLPLKCKDFKPYIVGSY
                     TREGYDTSTSRFELKSTIIEKYKDFGLDALPTYRGFNDNIDDEYTHLLTSGARISNAL
                     HSRLHDIKLSRILKNEANCDISSLDAKELGVISGDYIKITTKIGSLDVKVNVTDRVLY
                     KNIHMYHGYREADINSIICDNFDPYSGFPAYRSCKCKIEKMNR"
     gene            2207..2629
                     /locus_tag="PC471_003446"
     CDS             2207..2629
                     /locus_tag="PC471_003446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HDF2790531.1"
                     /translation="MKSLFKFNKELCTACGACLLACIDQNDNNIDNGELFRHVFTIEE
                     GENIEYFSLACIHCNEPKCLGVCPKNCFKKEEGFIVLDNQNCIGCKLCEKACEYGALI
                     YGNDGKANKCNGCIERYKCGMDSPCVMVCPTGALKFNK"
     gene            2803..3378
                     /locus_tag="PC471_003447"
     CDS             2803..3378
                     /locus_tag="PC471_003447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HDF2790532.1"
                     /translation="MVKVLAFMGSPRKKGNVDTILDEIIKGVNDNNVEVKKYYLNDMN
                     IKGCQGCLYCRKVPTCAFKDDMIDIYEDIKSAEYIIIGSPVYICQVSAQTKLLLDRLY
                     PLTEINKSKHIPRFGQKKLIMVYTQAAPISFLFKKYFKYTAKHLKGMGLNHYKTIVVT
                     KAFERNSTKKNFKVLKKAYKLGMKIAIDING"
     gene            complement(3503..4219)
                     /locus_tag="PC471_003448"
     CDS             complement(3503..4219)
                     /locus_tag="PC471_003448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HDF2790533.1"
                     /translation="MQQGSDYLKKFLEKHIDLLKKIPLFEGIKSDELEEMLECLGVVD
                     KTYPKDSIIFSVGSEVTSIGIMLKGSAHIIKEDIEGNRNIVAELLPGDLFGEVFACTR
                     LHKSNVTVTTTSSCEVLFIKFKSVTGICSSACVFHNRLVENMLQLIAEKNILLHNKIE
                     LLSRKTTREKLLMYFSKQIEQTGSHQFTIPFSRNEMADFLCVDRSSMSRELGKMRDEG
                     LLSFNKNKFEIFYFQNKLDI"
     gene            4384..5109
                     /locus_tag="PC471_003449"
     CDS             4384..5109
                     /locus_tag="PC471_003449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HDF2790534.1"
                     /translation="MMRKIIKIDEEKCNGCGLCVEACHEEAIGMVNGKAKLLRDDYCD
                     GLGDCLPTCPTNAISFEYREAAEYDEAAVKANMEAKKAQKKTLACGCPGSQSKSINRE
                     VSNSTSISNDIVEIKGSQLNQWPVQIKLVPTNASYLKNASFLIAADCTAYAYGNFHNK
                     FMKNKVTLIGCPKLDEVDYAEKLTEILKENDIKNIVVTRMEVPCCGGIVNAVKTALQN
                     SGKMIPWQIVTISVDGKIVDGEI"
     gene            5169..6761
                     /gene="hcp"
                     /locus_tag="PC471_003450"
                     /gene_synonym="priS"
     CDS             5169..6761
                     /gene="hcp"
                     /locus_tag="PC471_003450"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HDF2790535.1"
                     /translation="MEKMFCFQCEQTAGGKGCTINGVCGKKGGTANLQDELTGVLIGL
                     SRATIGNKNRPTSETDKIMIEGLFTTITNVSFDDEAIKRQIEKTEVEKSKLVPRCSDC
                     STTCNRNDNYDMKDLWNDNEDIRSLKSLILFGLRGMAAYAYHAMVLGYTDKDVNEFFY
                     EGLIAVGGDLSIDELLGLVMKTGEINLKCMELLDRANTETYGTPEPTQVSMKIEKGPF
                     IVVTGHDLYDLHKLLEQTKDKGINIYTHGELLPAHAYPKLKEYKHLKGNFGTAWQNQQ
                     KEFDNIPAPILFTTNCLMPVKDSYRDRVFTTEVVAYPGMVHIDEDKDFSSVIEKALEL
                     GGYREDVHMTGINGGDTLTTGFARGTVSSVQDKVVDAVKSGAIRHFLLVGGCDGAKPG
                     RNYYTEFVKQAPKDTVILTLACGKYRFNDLDLGDIGGLPRLMDMGQCNDAYSAIKVAV
                     SLANAFECGVNDLPLSMVLSWYEQKAVCILLTLLHLGIKNIYIGPSLPAFLSKNVLDI
                     LVDKFALTPISTPEEDMKKILG"
BASE COUNT         2714 a          787 c         1247 g         2203 t
ORIGIN      
        1 atttgaataa aaagaatgta taataagtat aataatttgt atttattatt gggaaaggaa
       61 aatgttaata tggatagtta taaacaactt aaagctaaaa taccctgtaa ggaaacaggt
      121 atagaaataa agaggacttt atgtgatatt tgttcgccag gagtacattg tggaattgat
      181 gcatatgtta aagatggtaa agtaataaaa atagaaggta caaaagaaca tccaatgaat
      241 aaagggttgt tatgtaccaa aggtctttgc aatagagaat atatctatag agaagataga
      301 ataaaaacac ctctaaaaag aattggaaaa agaggtgaag gaaaatttaa accaatatca
      361 tgggatgaag cttatagaga aatagcagaa aaattaaata gagtaaaatc tgagtatgga
      421 gcaaagcatg tagctttttt tacgggttat ggaaagtggt atagaccttt tttacaaagg
      481 tttgcatctt catttggttc acctaattat ggtacagaat ctagttcatg ttcaacatca
      541 gcacagatgg ctggaaatgt tgctactgga agaaacttta caatgcaaga tatggagaat
      601 gcaaaaactt atttagggtg gggatttaat ccatactact ctaaattttt aaatagtgtg
      661 aatctaataa aattaaaaga aaaaggtcta aaatttataa ttgtagaccc tagaataaca
      721 ccgacaactc aaaaactagc agatttacat ttaagaatta aacctggaac tgatggagct
      781 ttagctcttg gaatggcgaa tctatttata caaaaggatt taatagatag agaatacata
      841 gaaaagcatg tacatggatt taatgaatat aaagaatatg taaaattatt tgatttagat
      901 actgtaagta gaattacagg agtttctaaa gataatatag taaaagctgt tgagatgttg
      961 tcacaaggtc cattttgtat aaatgaaagt gcagctccaa tagttcatca tcaaaatgga
     1021 tttcaaaatt atagagcaat gatgtcttta tccgctataa caggtaatta tgatagaata
     1081 ggtggaaaca taccaaaaca agattacttt gctggtaaga taggtggatt ccaaacacta
     1141 gaaaaagagt ttgtaaaaga agttctacct aaaaatataa atgaaagaat tggtggagaa
     1201 agatttcctc tatggaatga aatggtgact caatttcaag ctatggattt acctaggcaa
     1261 atagaagaaa agacaccata tgaaataaga gctttgtttt gtcatgggat aaatgctaga
     1321 atgtttccta attcagggag actttttaaa gcgttgaata gtgtagattt ttttgtgaat
     1381 atagatttat ttatgacaga tacatcaaaa tatgcagata tacttttacc tgcatgtaca
     1441 tcatttgaga gagaagaatg taagagttat ccaggagggt atattacata taccaaaaaa
     1501 gttattgata aattatacga ttcaaaatca gatgtagaaa tactttcaga tttagcaaat
     1561 gctatgaata tagatgatga tttattaaaa gcaggttatg aagcaagtgt aagacatatg
     1621 tttaaaaata catgtgtaga tgtagataaa cttaaagaaa gtgatttacc attaaaatgt
     1681 aaagatttta agccatatat agtaggttca tatacaagag aagggtatga tactagcact
     1741 tctaggtttg aattaaaatc tacaattatt gaaaaatata aagattttgg gctggatgca
     1801 ctaccaacat atagaggatt taatgataat atagatgatg aatatactca tttattgaca
     1861 tcaggagcaa gaatttcaaa tgcactacat tcaagacttc atgacatcaa actttcaaga
     1921 atattaaaaa atgaagctaa ttgtgatatt tctagcttag atgcaaaaga attgggtgtt
     1981 atcagtggag attatataaa aataactact aagataggaa gtttggatgt aaaagtcaat
     2041 gttactgata gagtcttata taagaatata catatgtatc atggatatag agaagctgat
     2101 ataaatagta ttatttgtga taattttgac ccttatagtg gattccctgc gtatagaagt
     2161 tgtaagtgta agattgaaaa aatgaataga taaatggaga gatataatga aaagtttatt
     2221 taaatttaat aaagaattat gtacagcatg tggagcatgt ttgcttgcat gtatagacca
     2281 aaatgataat aatatagaca atggtgaact ttttagacat gtatttacaa tagaggaagg
     2341 tgaaaatata gaatattttt cattagcatg tatacattgt aatgagccaa aatgtttagg
     2401 tgtatgtcct aaaaattgtt ttaaaaaaga agaaggattc attgtattag acaatcaaaa
     2461 ctgtataggg tgtaaacttt gtgaaaaagc ttgtgaatat ggagccttaa tttatggaaa
     2521 tgatggtaag gcaaataagt gtaatggttg tattgagaga tataagtgtg gtatggattc
     2581 accttgtgta atggtatgcc ctacaggagc attaaaattt aataaataat tgtattaaat
     2641 tgctatacta cttttaaata attgtgttaa gaattatttt ttgatatact tttgtaatat
     2701 aaaatgtaaa taaaacaaat acaaagtata taaaatatat ttttagaata aagtgtgctt
     2761 tagattaaat ttagaaaaat aaattagaca ggagaactta atatggtaaa agtattagcc
     2821 tttatgggca gtccaagaaa aaaagggaat gtagacacaa tacttgatga gataataaaa
     2881 ggtgtaaatg ataataatgt tgaagttaaa aaatattatc taaatgatat gaatattaaa
     2941 ggatgtcaag gctgtctata ctgtagaaaa gttcctactt gtgcatttaa agacgatatg
     3001 attgacatat atgaagatat aaaaagtgct gaatatataa taattggttc gccagtatat
     3061 atatgtcagg tatcagctca aacaaagcta cttttagata gattgtatcc tttaacagag
     3121 ataaataagt ctaaacatat acctaggttt ggtcaaaaaa agttaatcat ggtctatact
     3181 caagcagctc ctatttcgtt tttatttaaa aaatatttta agtatactgc aaaacattta
     3241 aagggcatgg gattaaatca ctacaaaact atagttgtaa ccaaagcatt tgaaagaaat
     3301 tccactaaaa aaaattttaa ggtattgaaa aaagcctata aacttggaat gaaaatagcc
     3361 attgatatca atggatagat aaaagtttaa tttatagtat agctaaagga gcattaatta
     3421 ttaatactcc ttttttgtat aaaggtttct agaaaataac taataaaaaa caatattatt
     3481 tgaatacaga tattatacaa gcctagatat ctaacttgtt ttgaaaataa aagatttcaa
     3541 atttattttt gttaaaagat aacaagcctt catctcgcat cttgccaagc tctcttgaca
     3601 tagaacttct gtcaacacat aaaaaatcag ccatttcatt tcttgaaaag ggaattgtaa
     3661 actggtggct acctgtttgt tctatctgtt ttgaaaaata cataagcaat ttctcgcgag
     3721 ttgttttacg agaaagtaat tctattttat tgtgaagtaa aatgtttttt tcagctatta
     3781 attgtagcat attttcaaca agcctattat gaaatacaca tgcagatgaa caaataccag
     3841 ttacactttt aaactttata aataaaactt cgcaagaaga tgttgtggtt actgtaacat
     3901 tacttttatg tagtctagta catgcgaata cctctccaaa taaatctcca ggcaagagtt
     3961 cagctacaat attcctattc ccttcgatat cttcctttat tatatgagca cttcctttaa
     4021 gcataattcc aatagaagta acttcacttc caacagagaa tattatacta tcttttgggt
     4081 aagtcttatc tacaacacct aaacattcca acatttcttc aagttcatca cttttaattc
     4141 cttcaaatag aggaattttt ttaagtaagt ctatatgctt ttctaaaaat tttttcaaat
     4201 aatcacttcc ttgttgcata tgcaaccgat aatttgtttt cattgtacta tactaagttt
     4261 ataaaagcaa ggggatataa aaatcctact acaagaaatt tgaaataaag aaatataggt
     4321 attaaaaata aatatataag aaatcaatac tttaaattaa aaaaatttta tgaggtgata
     4381 taaatgatga gaaaaataat taaaattgat gaggaaaagt gtaatggttg tggactatgt
     4441 gttgaagctt gtcatgaaga agctatagga atggtaaatg gaaaagcaaa acttttaaga
     4501 gatgactatt gtgatgggct gggagattgt ctgccaactt gtccaacaaa tgcgatttca
     4561 tttgagtata gagaagcagc agaatatgat gaagctgcag taaaagcaaa tatggaagca
     4621 aagaaagcac aaaagaaaac tttagcatgt ggatgtccag gttctcagtc taagtcaata
     4681 aatagagagg tttcaaatag tacgtctata agcaatgata tagtagagat aaaaggttct
     4741 caacttaatc aatggccagt acaaataaaa ttagtaccaa ctaatgcatc atatttaaag
     4801 aatgcaagtt ttttaatagc agcagactgt acagcgtatg catatggaaa ttttcataat
     4861 aaatttatga aaaataaggt tacattaatc ggatgcccta aacttgatga agttgactat
     4921 gctgaaaaac ttacagagat tttaaaagaa aatgatatta aaaatatagt agtaacacgt
     4981 atggaagttc cttgctgtgg tggtatagta aatgctgtta agacagctct acaaaatagt
     5041 ggaaaaatga taccatggca aatagttact atatctgtag atggaaaaat tgtagatgga
     5101 gaaatttaat taagtgaatt atagaaaaat ataataaatt ttaataaata aaaagggagg
     5161 aataaaaaat ggaaaaaatg ttttgttttc aatgtgaaca aacagcagga ggtaaaggtt
     5221 gtactataaa tggtgtctgt ggtaaaaagg ggggtacagc taacttacaa gacgaactta
     5281 caggagtgtt gattggtctt tcaagagcaa ctattggaaa taagaataga ccaacaagtg
     5341 aaacagataa aattatgata gaggggctat tcactactat aacgaatgta agttttgatg
     5401 atgaagctat aaaaagacaa attgaaaaaa ctgaggtaga aaaaagtaaa cttgtaccta
     5461 gatgttcaga ttgttcaaca acttgtaata gaaatgataa ctatgatatg aaggatttat
     5521 ggaatgataa tgaggacata cgttcattaa aatcattaat cttatttgga ttaagaggta
     5581 tggctgccta tgcatatcat gcaatggtac ttggttatac agacaaagac gtaaatgaat
     5641 ttttctatga aggattaata gcagttggag gagacttgtc tattgacgaa cttttaggtc
     5701 tagttatgaa aactggagaa ataaacttaa aatgtatgga attgcttgat agagcaaata
     5761 ctgaaactta tggaacacca gaaccgactc aggtatctat gaaaatcgag aaagggccat
     5821 ttattgtagt tacaggacat gacctttatg atttacacaa attattagaa caaacgaaag
     5881 ataagggtat aaatatatat actcatggtg agttattacc agcacatgca tatccaaaac
     5941 taaaagaata taaacatcta aaaggtaatt ttggtacagc atggcaaaac cagcaaaaag
     6001 aatttgataa tattcctgct ccaatattat ttacaacaaa ctgtttaatg cctgttaaag
     6061 atagttatag agatagagta tttactacag aagtagttgc ttatccaggt atggttcata
     6121 ttgatgaaga taaagatttt tctagtgtaa tagaaaaagc tcttgaactt ggaggatata
     6181 gagaagatgt tcatatgact ggtattaatg gaggagatac attaactact ggttttgcaa
     6241 gaggtacagt ttcatctgta caagataaag tagtagatgc tgtaaagagt ggagctataa
     6301 gacacttttt attggttgga ggttgtgatg gtgcaaaacc tggaagaaat tattacactg
     6361 aatttgtaaa acaagctcct aaagacactg ttatattgac acttgcatgt ggaaaatata
     6421 gatttaatga tttagacctt ggggatatag gtggcttacc tcgtttaatg gatatgggac
     6481 aatgtaatga tgcttatagt gcaattaaag tggcagtttc attggcaaat gcttttgaat
     6541 gtggagtaaa tgatttgcct cttagcatgg tactgtcatg gtatgagcag aaagctgttt
     6601 gtatacttct tacactactt catttaggta tcaaaaatat ctatataggg ccttctttgc
     6661 cagcattctt atctaagaat gtattagata tattggttga taaatttgca ttgacaccta
     6721 tatctactcc tgaagaagat atgaagaaga ttttaggata aataaacata tctatactta
     6781 aagatattta tagttaaaat atttttaagt atgccacaat taagataagg ggttaaactt
     6841 aaaatttgat taaaaaatca attttgagat gaccctttat ttattttttg taataattga
     6901 aaaataaacc tgctttcagt atattctttt taagaatctt tgtattaaat t
//
LOCUS       DAMJYR010000173         6904 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.178, whole genome shotgun sequence.
ACCESSION   DAMJYR010000173 DAMJYR010000000
VERSION     DAMJYR010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6904)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6904)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6904
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.178"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            132..272
                     /locus_tag="PC471_003451"
     CDS             132..272
                     /locus_tag="PC471_003451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2790536.1"
                     /translation="MEKSNTTVTKKEKIEDIGMKLEHLLSEDITFYELLLEILYKEKE
                     EN"
     gene            complement(269..640)
                     /locus_tag="PC471_003452"
     CDS             complement(269..640)
                     /locus_tag="PC471_003452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2790537.1"
                     /translation="MESLGDRIANLRKELDINQKELATKVGITEASLSRYENNLREPK
                     SEIIVRLAKALETSTDYLLGVNDNTKISKEDKLIIENLSVSEKTKKLLEKIYSLEKED
                     REAIEKMIDNAYLKRFLKEEN"
     gene            1189..1446
                     /locus_tag="PC471_003453"
     CDS             1189..1446
                     /locus_tag="PC471_003453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2790538.1"
                     /translation="MIIIRSQDKTNLMQINQIKIDGSQVYAVFESKIDTVKIGDYENN
                     TRAIQVLDNIQNFIENGTKYDYITSNKVRYNVNKIFQMPAK"
     gene            complement(1556..2236)
                     /locus_tag="PC471_003454"
     CDS             complement(1556..2236)
                     /locus_tag="PC471_003454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HDF2790539.1"
                     /translation="MIWTFYSFICVILPCLIYQIVVIKRKNLKVEKNVMIHLVWVYIF
                     LLYIYLALSKAGIGSIWDIGRYSGLFRIDEINLIPFDSVGILTYILNIIMFMPLGFLL
                     PLIWKNFRNIINVSLTGLGFSLAIELCQLFNLRATDIDDLMMNTLGAVLGYFIWVGVN
                     NLFNLIKKKDIFPSIYKNIIQDEIAITIDDDISKKITSLYKNEAIIYLILAVLGEFLL
                     FNWSIIPY"
     gene            2496..2795
                     /locus_tag="PC471_003455"
     CDS             2496..2795
                     /locus_tag="PC471_003455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2790540.1"
                     /translation="MKWIKWYSITCICIFIVVAFYMFIFPNKIETIDTSSAYSFVEKK
                     VPNSAVYQGYKKNPVDGTTTIYYSYDNSTHIVRLSHPEDYSRKINWDKVSNIRFD"
     gene            3119..4774
                     /gene="ilvD"
                     /locus_tag="PC471_003456"
     CDS             3119..4774
                     /gene="ilvD"
                     /locus_tag="PC471_003456"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020457899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HDF2790541.1"
                     /translation="MRSDIKKGIEGAPKRALMYGMGLTKEEIERPLIGIVNAQNEVIP
                     GHLHLDEIAEAAKNGVRMSGGLPLEFPAIGVCDGIAMGHVGMNYSLASRELIADSIEA
                     MAMAHGFDALVLIPNCDKIVPGMLMAAARLNIPSIVVSGGPMLPGKKNGKVYDFNSAM
                     EGVGACKDGTVSEEELEELAMNSCPGCGSCSGLFTANSMNCLTEALGMGIPYNGTAAS
                     HSGERKRIAKYAGMYVMELLKNDIKPRDILTIDAFKNAIAVDMAMAGSTNTVLHLPAI
                     AYESGIELNLDFFDEISEKTPCLTKLSPSGKHHIEDLHMAGGIPAIMNELSKINGINL
                     DCKTVTGKTIGENIRNCEIENEEVIHTLKNPYSNQGGLAILKGNLALNGAVVKKSAVA
                     EEMLVHEGPARVFNSEEEAVNAIFGKKINKGDVIVIRYEGPKGGPGMKEMLSPTSAVA
                     GMGLDKHVALLTDGRFSGATRGASIGHISPEAMEGGLIGLVEEGDIISINIPDKKLEL
                     KVDEVEIENRKLKFKPLEPKIKHGYLSRYAKLVTSANTGAVLK"
     gene            5118..5747
                     /locus_tag="PC471_003457"
     CDS             5118..5747
                     /locus_tag="PC471_003457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDF2790542.1"
                     /translation="MNREEKSKNSKEKIIQSAFSLFSSKGYDSTSTQDIINLSGLSRG
                     AMYHHFKTKEDILRSVTKELYSQMNNFLEHLVADDTLTANEKIIELVVHSANDYTRRK
                     MVHCSWLEKIPFALIEEVRNLNNVVAPNIAKIIKQGVENKEFSCEYPEELAEMLVFSI
                     DILLDPVLFKREYSEVCNRLDFLLFMLKKMDIPLIDEYGIQKFKDLFKQ"
     gene            5773..6585
                     /locus_tag="PC471_003458"
     CDS             5773..6585
                     /locus_tag="PC471_003458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDF2790543.1"
                     /translation="MFYNAKSCQMMIGSNTVNYIEFGSGKKTLIILPGLGDGLFPLHG
                     KIQAIAFAFRYKQFTKDYKVYVFSRKNQITEKYSTRDMAKDQADIMKKLGIMKAEVMG
                     VSQGGMIAQYLAIDYPELVEKLVLAVTSSKQNDTIQNVICSWIDMAKKQNYNDLMIDT
                     AKKSYSERYLKKYQLFIPFLGKVGKPKDFKRFIIQATSCIEHNAFSELNKITCPTLII
                     GGANDKIVGNNASFHLVEKIKKSEIFIYEGLGHATYEEAQDFNERVLEFLNK"
BASE COUNT         2725 a          763 c         1082 g         2334 t
ORIGIN      
        1 tatctaggga aattttggat aatcaaaata tattttataa aatcaaaata caattacata
       61 aactttatta aaaatttgtt gggaatttct gactaacatt aagaacgtaa gtttgttggg
      121 ggatggtata tgtggaaaag tcaaacacta ctgttacaaa aaaagaaaaa attgaagata
      181 taggaatgaa attagaacat ttattgagtg aagatattac tttttatgaa ttattacttg
      241 agattttgta taaagaaaaa gaagagaatt aattctcttc tttaaggaat ctttttaaat
      301 aggcattatc aatcattttt tctatagctt ctctatcttc tttttcaaga gaatatattt
      361 tttctaagag cttttttgtt ttttcactta ctgataagtt ctctatgatt aatttatctt
      421 ctttgcttat ttttgtatta tcatttacgc ctaataaata atctgttgat gtttctaatg
      481 cttttgctaa tctaactatt atttcacttt taggttctct taaattattt tcataacgtg
      541 acaagctagc ctcagttata ccgactttag tagcaagttc tttttgattt atatctaatt
      601 cttttcgtag atttgctatt ctatctccta aactttccat atgtacatca ctccttaccg
      661 aaattataag ttattttgat aaaaagtaaa ataaaattac caaaaatata aaaaaaaaag
      721 gttaaaataa taaaatatat tgaaaattac catttgtata tgtataatat gaattataca
      781 aatggtaatt tgatgagagg tggtatgttg aaataagaaa ctgaattaat taataaatac
      841 atagaaagtt ttgaaaacct ttaattgaga tataaacaat atgtacaaaa ttttttgcaa
      901 gtaaaattac caaatgtata aaataaatca gtagcataga taattttatg ttttaataca
      961 ttagattatt taatttagtt ataatggtca aaaatgcaat ctacaaataa aacaaagtat
     1021 tatcaaaaaa gtattcttta agaaaatttt ttattagaga tgtaattgat ataaataatc
     1081 gtattaaaaa ataaaatact aagtttaaaa atttaaataa tctataaatc aattataaaa
     1141 tattatttat tagaaattaa aatgaattaa gtaaatggag gaattgatat gataataatt
     1201 agaagtcaag ataaaactaa tttaatgcag ataaaccaaa ttaaaataga tggtagtcaa
     1261 gtttatgcag tatttgaaag taaaattgat actgtaaaaa taggagatta tgaaaataat
     1321 acaagagcta tacaagtgtt ggacaatata caaaacttca ttgaaaatgg aacaaaatat
     1381 gattatataa catcaaataa agtcagatac aatgttaata aaatatttca gatgccagct
     1441 aaatagtgag tgtgcatttt aatttaataa ctaaagccca ctttcttttg tagtatgaaa
     1501 gagggcttta catatttgta aatatcgaag tacacttatt ttatcaataa attatttaat
     1561 aaggtataat ggaccaatta aacaataaaa attctcctaa aacagcaagt attaaatata
     1621 taatagcttc atttttgtaa agagaggtaa tttttttgct tatgtcatca tcaattgtaa
     1681 tagcaatctc atcttgaata atatttttat atatagatgg gaaaatatct tttttcttta
     1741 taagattaaa taaattattt acacctaccc atataaaata tcctaaaaca gcacctaatg
     1801 tgttcatcat taaatcatca atatctgtag ctcttaagtt aaacaattga caaagttcaa
     1861 ttgctaatga aaaacctaat ccagttaatg aaacattgat aatatttcta aaatttttcc
     1921 aaattaaagg tagtaaaaat cctaaaggca taaacataat tatatttaaa atatatgtta
     1981 agatgccaac tgagtcaaaa ggtattagat ttatttcatc aattctaaaa agcccactat
     2041 accttcctat atcccatatg ctaccaattc cagctttact aagtgctaga tagatatata
     2101 ataaaaaaat atatacccat actaaatgta tcattacatt cttttcaact ttcaaatttt
     2161 ttcgttttat aacgactatt tggtatatta agcatggtaa tataacgcaa ataaatgaat
     2221 aaaatgtcca tatcatttta ttttgtcccc tttcaaattt atgtttttag ttttataagt
     2281 actatcttta tattaaactg taagttttaa aaatctttta ataaaatctt aaaaatttat
     2341 taatttataa aaatatatta ttaagattaa aaaatatata taatcaaagt ttctacaatt
     2401 atatagttag ggatataaaa attttatcgt atgcataaat attataaata tatggaaata
     2461 ataatagtat attttggtaa agtaggagga attttatgaa atggattaaa tggtatagta
     2521 ttacatgcat atgtattttt atagtagtag ctttttatat gtttatattt cctaataaaa
     2581 tagaaactat tgatacttca tctgcatata gctttgttga aaagaaagta ccaaatagtg
     2641 ctgtgtatca aggatacaaa aagaatccag ttgatggtac tactacaatt tattacagct
     2701 atgataattc aactcatata gttagattat cacatccaga agactattca aggaagatta
     2761 attgggataa agtttcaaac atcagatttg attaatcttt aatatttata aaagtctata
     2821 tatctaaggc aaatatatta ttaatcttat tcatagttga gcactttcta ttatagaagt
     2881 gcctcttctg atttatatca taaaatatta ctcaacaatt tgtaaaatat attagtttat
     2941 aaaatatatt gaaaagataa tattatataa tacgattgtg taagatattg ataaaaaatc
     3001 gtaataaatt ttcattagta tatgctaata tttttttaca attttcgtaa ttaattaata
     3061 gaaaaatata ttataataat attatgaaaa tttaaaaaag taagaaagag gtaatattat
     3121 gagaagtgat ataaaaaaag gaatagaagg agctccaaag agagcgttaa tgtatggaat
     3181 gggtcttact aaagaagaaa ttgaaagacc attaataggt atagtaaatg cacaaaatga
     3241 ggtgattcct ggtcatctgc atcttgatga aatagcagaa gctgcaaaaa atggagtaag
     3301 aatgagtggt ggtttgccat tagaatttcc tgctataggg gtatgtgatg gtatagctat
     3361 ggggcatgtc ggaatgaatt attccctggc ttcaagagaa ttaatagcag attcaataga
     3421 agctatggct atggcacatg gttttgatgc tttggtactt atacctaatt gtgacaagat
     3481 agtgccaggt atgttaatgg ctgctgcaag acttaatata ccaagtattg ttgtgagtgg
     3541 agggcctatg cttccaggta aaaaaaatgg aaaagtatat gattttaact ctgcaatgga
     3601 gggtgttgga gcatgtaaag atggaacagt aagtgaagaa gaattagaag agttagccat
     3661 gaactcttgt cctggatgtg gttcatgttc tggtcttttc acagcaaata gcatgaattg
     3721 tttaacagaa gctttaggca tgggtatacc atataatggt actgctgcgt cacattctgg
     3781 agaaagaaaa aggatagcaa aatatgcagg tatgtatgtt atggagttac ttaagaacga
     3841 cataaaacct agagatattt taacaataga tgcttttaaa aatgctatag ctgtggatat
     3901 ggcaatggct ggttctacaa atacagtact tcacttacct gcaatagctt atgaatcagg
     3961 aatagagctt aacttagatt tttttgatga aataagtgaa aaaactcctt gtttaacaaa
     4021 attaagtcca agtggaaaac atcatattga agatttacat atggcaggag gaataccagc
     4081 tataatgaac gaactttcaa agataaatgg aataaattta gattgcaaaa ccgtaacagg
     4141 caagactata ggggaaaata taagaaattg tgaaatagaa aatgaagaag taatacatac
     4201 attaaagaat ccatatagta accaaggtgg gcttgcaata ttgaaaggaa atcttgctct
     4261 aaatggagct gttgtaaaaa agtcagcagt tgcagaagaa atgttagttc atgaaggacc
     4321 agcaagagtt tttaattcag aagaagaagc tgtaaatgct atttttggta aaaaaataaa
     4381 caaaggtgat gttatagtta taagatatga aggtccaaag ggtggtccag gaatgaaaga
     4441 aatgctatct cctacatcag cagttgcagg aatgggactt gataagcatg tagcacttct
     4501 tactgatggt cgtttttcag gggcaactag aggggcatcc ataggccata tttctccaga
     4561 agctatggaa ggtggtttaa tcggactagt tgaagaagga gatataattt ctataaacat
     4621 accagacaaa aaattagagt taaaagtaga tgaagttgaa atagaaaata gaaaattaaa
     4681 atttaaacct ttagaaccga aaataaagca tggatactta agtagatatg ctaaactggt
     4741 aacatcagca aatacaggag cagttttaaa atagtagact atatttatgt aaattagaaa
     4801 aagttaagaa aatattaatt ggatatctta ggtgttatag cactaatgag gtatcctttt
     4861 tattttgtat gaaaaacata gcaaaatctt gattatcttt gaaaacaata catctctatt
     4921 ataaggaata cgagtacagc atagagatgt atcttttaga ccttgttgag gtaatacaac
     4981 tttttaaaag tttgatgtgg aatgcttttc gttccttctg aactatgaag aaattgaaag
     5041 taagttaatt cttgacataa taaatattaa aatgtataat aaaaaacaaa cagacagtct
     5101 gtttttagga gggaaatatg aatagagaag aaaaaagtaa aaatagtaaa gaaaaaatta
     5161 tccaatcagc attttcacta ttttcgtcta aaggatatga ttcaacatct acacaagata
     5221 ttatcaattt atctggtcta tctagaggtg caatgtatca tcactttaaa actaaagaag
     5281 atatactgag aagtgtcaca aaagaacttt actcacaaat gaataatttt ttagagcatc
     5341 ttgttgctga tgacaccctt acagcaaatg aaaaaataat agaattggtt gttcatagtg
     5401 cgaatgatta cacacgtaga aaaatggtac attgtagctg gttagaaaaa atcccattcg
     5461 ctttaataga ggaagttcgt aatctcaaca atgtagttgc acccaatatt gctaagataa
     5521 ttaaacaagg tgttgaaaat aaagaatttt cttgtgaata tccagaggaa ttagctgaaa
     5581 tgctcgtttt tagtattgat attttacttg accctgtatt atttaaacgt gaatacagtg
     5641 aagtatgtaa taggttggat tttttattat tcatgttaaa aaaaatggat attcctctaa
     5701 ttgatgagta tggtattcaa aaatttaaag atttatttaa acaatgataa taggaaaagt
     5761 gaggaataga ctatgtttta taatgcaaaa agttgtcaaa tgatgatagg aagtaataca
     5821 gtgaattata ttgaatttgg tagtggtaag aagacactaa ttattttacc aggattagga
     5881 gatggtttat ttcctttaca tggaaaaata caggcgattg cttttgcttt tagatataag
     5941 caatttacaa aagattataa ggtttatgta tttagtagaa aaaatcaaat tacagaaaaa
     6001 tattccacaa gggatatggc taaagaccaa gcagatataa tgaaaaaact tggaattatg
     6061 aaagcagaag taatgggtgt ttctcaaggt ggaatgatag ctcaatatct ggcaattgat
     6121 tatcctgagt tagttgagaa acttgtgttg gcagttactt cttcaaagca aaatgatact
     6181 atccaaaatg ttatttgtag ttggattgat atggcgaaga agcagaatta caatgattta
     6241 atgattgaca cagcaaagaa atcctattct gaaagatatt taaaaaaata ccaattattt
     6301 attccttttt taggaaaagt aggtaaacca aaggatttta aacgatttat tattcaagcg
     6361 acctcttgca tagaacataa tgccttttcc gaattaaata aaataacatg tccaacctta
     6421 attataggag gtgctaatga taaaattgtt ggaaataatg cttcttttca tttagtagaa
     6481 aaaattaaaa aaagcgaaat atttatctat gagggattgg gacacgctac atatgaagaa
     6541 gcacaggatt ttaatgaaag agttttagag tttttaaata agtaaacacc tttgttaaaa
     6601 atatacatat gaagattgga aatttaatgt taaaaataga aacatgaaaa tatgcttaac
     6661 tggtattttt actattcata accaattttt gatacattat ctactataaa tacaaatata
     6721 gcttcaatgt gattatatat tgttgtattg gtaaagcact tatacaaaca gaggaatttt
     6781 gtaaattcat attataccca catttgttaa cttatgaaaa tataatcaaa atttttatga
     6841 gctttttata taaaaaatat aatgtttgta tacaaaaata ttaaagacat tttagtaagt
     6901 tttg
//
LOCUS       DAMJYR010000174         6896 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.179, whole genome shotgun sequence.
ACCESSION   DAMJYR010000174 DAMJYR010000000
VERSION     DAMJYR010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6896)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6896)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6896
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.179"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            71..1195
                     /locus_tag="PC471_003459"
     CDS             71..1195
                     /locus_tag="PC471_003459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3810 domain-containing protein"
                     /protein_id="HDF2790544.1"
                     /translation="MSKKTKYFSLILFPIALLLNFIASKVPNIVEKYYSQFIDKIIVQ
                     ILSKVSGIFPFSLYEITMYIIVISTFLFLCCTLSTIFNKKKKLNIYLKNSILNILSIV
                     SIVYFLFVVLWGINYNRIPLETTLINNYNLKNNTSIQSKQYSVKELTKLYKFLVIKAN
                     ETRKLTLQDKNGIVKSNTDYNGVINRAQLGYDNILDILPGVSDSYSKPKYIISSNLMC
                     YTGITGIYFPFTGEANVNIAIPDLYIPCTVEHEMAHQRGFASEDEANFIAYLTSIKHP
                     NIDFNYSGYILALNYTASALSKVDYNAYVDISAGISDSVRRDLKNESEFWQKYEGKIN
                     EISNEFNNSYLKANGVTEGTQSYGKMVDLLLTYYELYPYN"
     gene            complement(1669..2493)
                     /locus_tag="PC471_003460"
     CDS             complement(1669..2493)
                     /locus_tag="PC471_003460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2790545.1"
                     /translation="MRYKKISSLPILVVALLITGTTTTFSLSTTNSEKTNVYLANTVS
                     TVTKKVSDIQSSKGLVYNENIPLFINNSKIQYDDTPYHWPSNVISLTNSSEKAIMDYE
                     ITCLAYDKNGKPLELYWDAQNVAADGEVGSVGFSPAGVDYGIVTGISPVSPKSYSHTY
                     RKMQQSPPQDIISMFEKQQGKAWVENWLKEWKQMEKEYAKQNAIAPGKNQNDAFLLFD
                     KWKQSTGEHGVKYIISCVKQVTFNDGSVWKNSAYENWLKSFQGKEVSNSVLENYYK"
     gene            complement(2841..3896)
                     /gene="ispG"
                     /locus_tag="PC471_003461"
                     /gene_synonym="gcpE"
     CDS             complement(2841..3896)
                     /gene="ispG"
                     /locus_tag="PC471_003461"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HDF2790546.1"
                     /translation="MSYERRKTREVSVGAVKIGGENPISIQSMTNTDTRDADATIAQI
                     KRLEEAGCDIVRVAVPDIKAAKNIAKIKSSVNIPIIADIHFDYKLALEAIEQGVDGIR
                     INPGNIGSIERVKMVVEKCKERNLKIRIGVNGGSLEKELLKKYGSATAEALVESAMGH
                     IKILEDLDFHNIVISLKSSDIYKTVDAYELISKKVDYPLHIGITESGSVHKGTIKSSI
                     GVGALLLKGIGDTVRISLTGDPVEEVIVGKQILRSLGLLNDKIKVISCPTCGRCNIDL
                     ISVVNEVEEKIGSMEKNITVAIMGCAVNGPGEAREADIGIAGGKGEGLLFKKGEIVRM
                     IDGNKLVDELLEEIEKL"
     gene            complement(4079..5083)
                     /gene="rseP"
                     /locus_tag="PC471_003462"
     CDS             complement(4079..5083)
                     /gene="rseP"
                     /locus_tag="PC471_003462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HDF2790547.1"
                     /translation="MTIIAALILFSIIVLIHELGHFIFAKRSGIKVNEFSIGMGPKIY
                     SVKKDTEYSIRALPIGGYVSMEGEDEEQISPNSFGNKSILQRFSTIVAGPIFNIILAA
                     ILLVPVFLYIGSPTTKLGKIMPDTPAQAVGLQVGDKINKINGNSVKTWDEVANIINTS
                     SGGELKLSITRDGSDKVVNVTPKNNNGKYEIGIQPQREKDFLGSIVNACKTTVDMTKQ
                     MLTFLGQMITGRVPGGIGNAVAGPVGVIGMVSDAARTGIINVVYLAAVISLNLGIVNL
                     LPIPALDGWRILMLLLEAVRGGKKLDPNKEGMINVVGFGALMLFMLFITYKDILRLFQ
                     "
     gene            complement(5096..6250)
                     /locus_tag="PC471_003463"
     CDS             complement(5096..6250)
                     /locus_tag="PC471_003463"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HDF2790548.1"
                     /translation="MKKISILGSTGSIGKQTLDVVREHRDKFEIVAISANSNIELLLE
                     QIVEFKPKYVTVFEENKALKLKEMLPKNIEIEVLAGMEGLKIISSLDEVDVLLTAVVG
                     MIGLVPTLCAIKKGIDIALANKETLVTAGELVMKEAEKYNVNILPVDSEHSAIFQCLN
                     GENKKNIEKIILTASGGPFRGKKKGELANITKNEALKHPNWSMGRKISIDSSTLMNKG
                     LEVIEARWLFGVEQENIDVVVHPQSIIHSMVQYTDSSIIAQLGCPDMRLPIQYALTYP
                     DRMESSFERMNFSKFSTLTFEEPDLETFPCLKLAYECLKMGGTYSSVLNSANEVLVSE
                     FLEDKIGFYDIPYYIEKTLEVHSSISEPTLEEILETDRWSRAYVANLIKK"
     gene            complement(6414..6617)
                     /locus_tag="PC471_003464"
     CDS             complement(6414..6617)
                     /locus_tag="PC471_003464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF2790549.1"
                     /translation="MISYAPLWKLLIDRKIKKMEFVNISGISISVLGRLGNDKSVSMD
                     TMEKICLALDCKIEDVVEIKKGI"
BASE COUNT         2303 a         1206 c          710 g         2677 t
ORIGIN      
        1 acttatttta caatctttct tcaattgata tataatatgt ttatataaac taatgtatgt
       61 gaggtgaaat atgagcaaaa aaacaaaata tttttcttta attctctttc ctattgcttt
      121 gcttttaaat tttatagcca gtaaagttcc aaatattgtt gaaaaatatt actcccaatt
      181 catagataaa ataattgtac aaatattgag taaagtttct ggaattttcc cattttctct
      241 ctatgagatt acaatgtaca taatagttat atctaccttc ttatttttat gttgtacttt
      301 atctacaatt tttaataaga aaaaaaaatt aaacatatat ttaaaaaatt ccattttaaa
      361 tatattatcc attgtatcaa tagtctactt tctttttgta gtgctttggg gaataaacta
      421 taataggatt ccactagaaa ccacacttat aaataactat aatttaaaaa acaatacctc
      481 tatccaatca aaacaatata gtgttaaaga gcttactaaa ttatataaat tccttgtaat
      541 taaagcaaat gaaactagaa aactcacatt acaagataaa aatggtattg taaaatctaa
      601 tacagattat aatggtgtca taaatagagc tcaactagga tacgataata tcttggatat
      661 attacctggt gttagtgata gttattctaa gcctaagtat attatttcat caaatctaat
      721 gtgctataca ggaataactg gtatatactt tccttttaca ggtgaggcaa atgtaaatat
      781 agcaatacct gacttatata ttccttgtac agttgaacat gaaatggcac atcaaagggg
      841 atttgctagt gaagatgaag ctaattttat agcgtatctt acttctataa aacaccccaa
      901 tattgatttt aactattctg gatatatttt agctttaaat tatactgctt ctgctttaag
      961 taaggttgat tataatgcat atgttgatat ttctgctggc atttcagatt ctgtccgtag
     1021 agatttaaaa aatgaaagtg aattttggca aaaatatgaa ggtaaaatta atgaaatttc
     1081 taatgagttt aacaattctt acttaaaagc taatggcgtt actgaaggta ctcagagtta
     1141 tggtaaaatg gttgatttat tgctaactta ttatgaatta tatccatata actaaacact
     1201 acaattcaaa attttattca tatacagctg atattaaatc agctgttttc tatttaaata
     1261 aattatttaa cttcaagcaa tcttatataa tttgatagta cttatactat ctgtataaca
     1321 tttcaattct aactatttta gttaaaacat ttctattatc ttttttaata aatgttttaa
     1381 ctaaaatttt taaaacataa aacttccaca ttatttcact atctatataa aaatctaaaa
     1441 attcacatat aaaattacat aactaaaaaa tcataaattt atagaaaaca tatattcaaa
     1501 ataaaaaagc atgtatcaaa acttgacaca tgcttataat cttatgtata tatatcattt
     1561 tgtcacaata aaaatttact tgtaacactg atatttttaa acaaaatagt ccttatttaa
     1621 ttaaattaac attaattaaa ttcgtactca ttttgctatc attaaacatt acttatagta
     1681 attttctaat acactatttg aaacttcctt cccttgaaaa cttttcaacc aattctcata
     1741 tgctgagttt ttccatacac taccatcatt aaatgttact tgttttacac aagatataat
     1801 gtactttaca ccatgctctc cagttgattg tttccattta tcaaacaata gaaaggcatc
     1861 attttgattc ttacctggtg ctattgcatt ttgctttgca tattctttct ccatttgttt
     1921 ccattctttc aaccaatttt ctacccatgc cttgccttgt tgtttttcaa acatacttat
     1981 tatatcctgt ggtggtgatt gttgcatttt tctataagta tgtgaatatg attttggaga
     2041 cacaggagat atgcctgtaa caataccata gtctacacca gctgggctaa atccaacact
     2101 tcctacttcg ccatctgctg ccacattttg agcatcccaa tatagctcaa gtggttttcc
     2161 atttttatca tatgctaagc atgtaatttc ataatccata attgcctttt cactgctatt
     2221 tgttaagctt ataacattgc taggccaatg atatggagta tcatcatact gtattttgct
     2281 attatttata aataatggaa tattctcatt ataaacaaga cctttactac tttgaatatc
     2341 tgataccttc tttgtgacag tacttaccgt atttgctaag tatacatttg ttttttctga
     2401 attggtcgta ctaagagaaa atgttgttgt cgttccagta atcagcaaag caacaactaa
     2461 aataggtaag ctagatattt ttttatatct cacaattctt cacctctttt tctattaact
     2521 tataataaaa caatttacca aaacattaaa atctatgtta acataattta ccaactgttc
     2581 tagtaactat atcctatcat agttactatc aagtttcaat actattgatg gaaattttca
     2641 ctatattaaa tcttttttta attttataaa tttacaatat gatttaactc atacatatat
     2701 agtcaaatta gaatataagt ttcttacatt taatgaagtt tataaaataa aaaatgacta
     2761 gataattaaa tactgtaaat aaaaacttat tacaaaagta tttaaaaatc tagtcattta
     2821 atatatatat gaattgtata ctataatttt tcaatctctt ctagcaactc atcaactaat
     2881 ttgttaccat caatcattct aactatttca ccctttttaa ataaaagacc ttcaccttta
     2941 cccccagcaa tacctatatc tgcttctctg gcttcaccag gtccatttac agcacagccc
     3001 ataattgcaa cagtgatatt tttttccata ctaccaatct tttcttcaac ctcatttaca
     3061 acactaatta agtctatatt acatctacca catgtagggc atgatataac tttaatctta
     3121 tcatttaaaa gtcccaaact tcttaaaatc tgtttaccaa caataacctc ttcaacaggg
     3181 tcgcctgtta aagatattct aacagtatct ccaattcctt taagaagaag tgctcctacc
     3241 cctatagaag actttattgt tcctttgtga acacttccag attctgtaat tccaatatga
     3301 agaggataat caactttttt tgatattaat tcatatgcat caacagtttt ataaatatcg
     3361 gatgatttta gagatataac tatattatga aaatctaagt cttcaagaat cttaatatgt
     3421 cccattgcac tttcaactaa tgcttcagca gttgcagagc catacttttt taaaagttct
     3481 ttctctaaag aacctccatt aactccaatt ctaattttta aatttctttc cttgcatttc
     3541 tcaactacca tcttaactct ctctatactt ccaatattac caggatttat tcttattcca
     3601 tccacacctt gttctatagc ttcaagggct agcttataat cgaaatgtat atctgcaata
     3661 attggtatat ttacactaga ctttatcttt gctatatttt tagcagcttt aatatcagga
     3721 acagcaactc taactatatc acaaccagct tcctctaatc tctttatttg agctattgta
     3781 gcatctgcat ctcttgtatc agtatttgtc atagattgta tacttatagg attttctcct
     3841 cctattttta cagctcctac acttacttct ctcgtctttc ttctctcgta actcatattc
     3901 cacctctata tattatttta tattgaatct ctttaaatat taaaatatta taacctacat
     3961 gtatattata aattaaaaag ctatctagtt aaataagaat aaatttcata tataaaaatt
     4021 aatttattct tatttaaaac tacatagcct attttcttaa aatctctaaa aaactaaatc
     4081 attgaaataa tcttagtata tctttatatg tgataaaaag cataaataac attaaagctc
     4141 caaaaccaac aacatttatc atgccttctt tatttgggtc gagtttttta ccacctctaa
     4201 cagcttctag taacaacata agtattctcc atccatcaag tgctggtatt ggtaatagat
     4261 ttactatacc taaatttaag cttatgacag ctgctaaata cactacattt atgatgcctg
     4321 tacgagcagc atctgatacc ataccaataa cacctactgg tcctgctaca gcattgccaa
     4381 tccctcctgg tacacgtcct gtaatcattt gacctagaaa tgttagcatc tgttttgtca
     4441 tatctacagt agttttacat gcatttacta ttgaccctaa aaagtctttt tctctttgtg
     4501 gttgaattcc tatttcatac tttccattgt tatttttagg tgttacattt acaactttat
     4561 cacttccatc tctagttata ctaagtttta attctccacc agaagaagtg tttattatat
     4621 ttgcaacttc atcccaagtc ttaacagaat taccattaat tttatttatc ttatctccta
     4681 cttgaagccc tacagcctgt gcaggagtat ctggcattat ctttcctaat ttagtcgttg
     4741 gagaacctat atacaaaaat actggaacta aaagtattgc tgctaatatt atattgaata
     4801 ttggtcctgc tacaatagta ctaaatcttt gtaatataga cttgtttcca aaagaatttg
     4861 gacttatttg ttcttcatct tcaccttcca tgctgacata tccaccaatt ggaagtgctc
     4921 ttatagaata ctctgtatct ttttttacac tatatatctt aggccccata cctatagaaa
     4981 actcatttac tttgatacca cttctttttg caaatataaa atgtcctaac tcatgtatta
     5041 atacaattat actaaatagt attaatgcgg ctattatagt cataaattca ccttcctatt
     5101 tttttatcag atttgcaaca taagctctac tccatctatc tgtttctaag atttcttcta
     5161 atgttggttc acttatgcta ctatgaactt ctaaagtctt ctctatgtaa tatggtatgt
     5221 catagaaacc aattttatcc tctaaaaatt cacttactaa aacttcattt gctgaattta
     5281 aaacagaaga ataagttcct cccattttta aacactcata tgccaatttt aaacatggaa
     5341 aagtttctaa atctggttct tcaaaagtta aagtactaaa ttttgaaaaa ttcattcttt
     5401 caaagctact ttccattcta tctggatatg taagagcata ctgtattggt agtctcatat
     5461 ctggacatcc taattgagca attattgagc tatctgtata ctgcaccatt gaatgaatta
     5521 tgctttgtgg gtgaactact acatctatat tttcttgttc tactccaaat aaccatctag
     5581 cttctatcac ttcaagtcct ttattcataa gcgttgaaga atcaatactt atttttcttc
     5641 ccatactcca atttggatgc ttcaaagctt catttttagt tatatttgca agttctccct
     5701 ttttctttcc tctaaatgga ccaccagatg ctgtaagtat tatcttttct atattttttt
     5761 tattttctcc atttagacat tgaaatatag cactatgttc actatcaaca ggaagaatat
     5821 ttacattgta tttttctgct tctttcatta caagttcacc agcagttacc aacgtttctt
     5881 tattcgctaa tgctatgtct attcccttct ttatagcaca aagagttggg actaatccta
     5941 tcatacctac tacagcagtt aaaaggacat ccacttcatc aagtgatgat attattttta
     6001 atccttccat tcctgctaaa acttctattt ctatattttt aggcaacatt tcttttaatt
     6061 ttaatgcttt attttcctca aaaactgtta catatttagg tttaaactct actatctgtt
     6121 ctagcaataa ttcaatatta ctgttagctg atattgcaac tatctcaaac ttatctctat
     6181 gttctctaac cacatccaaa gtttgtttac ctatagagcc tgtagaacct aaaattgata
     6241 tctttttcat ataataatcc tttctaataa taaatatacc ctgtcaaaac attataacac
     6301 tactaaaaca attattttta ctattcatag aaatttcata tattattcca cagattaaaa
     6361 ttaatctatt attttttgta ttttgtggaa tattataaaa tattctagta ttattaaatg
     6421 ccctttttta tttccacaac atcttctatt ttacagtcta gtgctaaaca tatcttctcc
     6481 attgtatcca tagaaacaga tttatcattt cctagtctgc ctaaaacaga aatacttata
     6541 cctgatatat ttacaaattc cattttcttt atttttctgt caattaacaa tttccataat
     6601 ggagcgtatg aaatcatgtt atcacctcgc aaaatatatt ctatcacatt ttatctattt
     6661 tggaaaattt tcatctattt agataaataa tcacctgctt aagtgtcaaa taattttatc
     6721 cttctaatat gaaaatttaa tactatacta aatgattggt atataacaca tatgatgcat
     6781 tattattaac atataaaaat ttttattggt gctaaattaa agtaatatac tattattata
     6841 tttgcagata tataagaaag attttattat atatttaaag tacttgatta taataa
//
LOCUS       DAMJYR010000175         6705 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.180, whole genome shotgun sequence.
ACCESSION   DAMJYR010000175 DAMJYR010000000
VERSION     DAMJYR010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6705)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6705)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6705
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.180"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     misc_binding    400..638
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            702..2621
                     /gene="thrS"
                     /locus_tag="PC471_003465"
     CDS             702..2621
                     /gene="thrS"
                     /locus_tag="PC471_003465"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HDF2790550.1"
                     /translation="MIKVALKDGSIKEFENAISVMDVAKSISEGLARNVVAASVNGEV
                     VGLDHIIDTDCDLNLFKFEDKEGKEVFRHTSAHILAQAIKRLYPEAKLAIGPSIENGF
                     YYDIDLDHRLVPEDLEKIEAEMKKIAKEDLKIERFELPRNEALELMKEQGEDYKVELI
                     SDLPESEIISFYKQGDFTDLCRGPHLPSTKKVKAVKLQSVAGAYWRGDENNKMLQRIY
                     GTSFEKNKDLEEYLHLLEEAKKRDHRKLGKELGLFMIPEEGPGFPMFLPKGMELKNEL
                     LKFWREIHRKAGYIEIESPIILNRKLWETSGHWYHYKENMYTVKIDDEDYAIKPMNCP
                     GGLIYYNSQLHSYRDFPMRVAELGRVHRHELSGALQGLMRVRAFTQDDSHIFMLPEQI
                     KDEIKGVANLIDGIYKTFGFEYNLELSTRPENSMGSDEEWEAAENGLREALEELGLPY
                     TINEGDGAFYGPKIDFHLKDCLGRTWQCGTIQLDMQLPRQFDNTYIGQDGEKHRPVMI
                     HRVAFGSIERFIGILIEHYAGKFPVWLSPTQVKILPISDKFMDYANEVKKELFDKGIR
                     VELDDRAEKIGFKIREAQLEKVPYMLIVGEKEVADNNVSVRSRDKGEIGSIKLDEFIA
                     SISKEIESRESIIQD"
     gene            3389..4480
                     /locus_tag="PC471_003466"
     CDS             3389..4480
                     /locus_tag="PC471_003466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HDF2790551.1"
                     /translation="MIEKVEEKSKIIKTSKEYKRTIIEEKDKEWTKKLNDILSRSSFS
                     NDELDKIKALIPKEILTNENVGEVVTEDGYAKPEYDEVFKSLFTLNNEYDLLVNFIND
                     ILKDAPYANRNIKPFTQIKRIIRVETDPTINYIGEKRPKLDILAEDEENNHINIEMQR
                     AFENDYLERAEYYLSRVHGRKLEEGKEYKEIGKTIGIHILNHVKYNHIEDYVNCLRLT
                     MDGHPDIYSSKTALYFIELPKIHKSDYIVNRIMLWGKLISNPSNLDVRVIAKNDSIIK
                     KALDRLKELGSNEEYLLNLKRGAYIMNKSRNFKEEIERETAIRVAKEKDYKIIIMLKK
                     NGFKLDDILNKFNDFDLSEDEIKEVYENN"
     gene            5150..6490
                     /locus_tag="PC471_003467"
     CDS             5150..6490
                     /locus_tag="PC471_003467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HDF2790552.1"
                     /translation="MIKSSLFWNIFIAISFMIEWFLCKKMLDYTSEKRISNKKINFCM
                     VGIIVFADIIYFFDVPPNIRVIISMIITIAFYKISYDVGFIKSVLISLIYWMLLIATD
                     ALSISLTVWLNSINDMSSMGTGDIYRIESIILGKILLIVSLLLYRALKIKLNIEINKR
                     TLFYIVIPIVANIASFFVIFEYIFNPGSGSLIHNFQFVMISVVLLISSITLVLIAKKL
                     QQYNTLVVENNMIKNTLKYENEYYEDIEKKYLKTRLLSHDMKNHILCINAMIKKGIDV
                     TSYLNNLQVEIQSNDLLFNTGNFILDAILSEKKEVCDKNDIELKIGINFSCCEAVELV
                     DICHIFSNILDNAIEACNKIDSGNRVICVKGDVMQNFYLIRVENTKINKIITKNGDIL
                     TDKKGSLHGLGIKSVKNSVNKYGGNVVIEYDDKKFVVKIAIPLRNVKKAVNHVI"
BASE COUNT         2660 a          608 c         1144 g         2293 t
ORIGIN      
        1 cttattaata gcatttagat attaattgta tttatttatt tgattgtgtt acttattaat
       61 agtatttaag tatttactgt actacttatt aaccatatta agtattgatt gtatttaagt
      121 atacaaaacc taaaaagagt acaaaaatta gaagttagtt ttaataaaaa ttagttttag
      181 tatttatcta gtcatatcta tcatgcatgt ggatacgttt taaaaatttt aaatttatga
      241 aaatattata atataactat aaatcaagag attaatttaa tataaaaata taagaatatt
      301 ggtttacagc tgattgagat gaaaaataat gggtaattaa tatattacat taatgttgat
      361 tgatgtataa ctatctgtta taatgaaaat atattaaaaa tcatagtgtt gaagaggaag
      421 agtagagtag aaaccattct cagagaattt gggtctatgg ctgagagccc atgtaatagg
      481 tctatttgaa aactaccttg gagctgagct tattagcagt atttgtattt atactatacg
      541 tgaaagtgct cgggtgacga ccttatagtc atcaagaggg ttgtatatac ttgtatacgc
      601 caacttgggt ggaaccgcga attaatatcg tcccaaacat atttggggcg attttttatt
      661 acaaaaaatc taaaaattat ttcaataagg aggaaaaaac aatgattaaa gttgctttaa
      721 aagatggctc aataaaagaa tttgaaaatg ctatttctgt tatggatgta gctaaatcca
      781 ttagtgaagg attagctaga aatgtggtag cagcgtctgt gaacggagag gttgtaggtc
      841 tggaccacat tatagatact gattgtgact tgaatttatt taagtttgaa gataaagaag
      901 gtaaggaagt ttttagacat acatcagcgc atatattagc tcaagctata aaaagacttt
      961 acccagaagc aaagcttgct ataggaccaa gtatagaaaa tggattctac tatgatatag
     1021 atttagacca tagattagtt ccagaggacc tagagaaaat agaagctgaa atgaaaaaaa
     1081 tagctaaaga agatttaaaa atagaaagat ttgaacttcc tagaaatgaa gctttagagc
     1141 ttatgaaaga acaaggagaa gactataagg tagaacttat ttctgattta cctgaaagtg
     1201 aaataatatc tttctacaag caaggtgatt ttactgattt atgtagagga ccacatttac
     1261 catctactaa aaaggtaaaa gctgttaaat tacaaagtgt agcaggtgca tattggcgtg
     1321 gtgacgaaaa taataagatg cttcaaagaa tatatggaac ttcttttgag aaaaacaaag
     1381 acttagaaga gtacttacat ttactagaag aagctaaaaa gagagaccac agaaaattag
     1441 gtaaagaatt aggattattt atgatacctg aagaaggtcc aggattccct atgtttttac
     1501 caaaaggtat ggaacttaaa aatgaattat taaaattctg gagagaaata cacagaaaag
     1561 ctggatatat tgagatagaa tctccaatca tattaaatag aaaattatgg gagacttcag
     1621 gacactggta tcattacaaa gaaaatatgt atacagttaa aatagatgat gaggattatg
     1681 ctataaaacc tatgaactgt cctggtggat taatttacta taactctcaa ctacattcat
     1741 atagagattt cccaatgaga gttgcagagc ttggtagagt tcatagacat gagttatctg
     1801 gtgcattaca aggattaatg agagttagag catttactca agacgattca catatattca
     1861 tgttaccaga gcaaattaag gacgaaataa aaggagtagc taacttaata gatggtatat
     1921 acaaaacttt tggatttgaa tataatttag aattatctac aagaccagag aactctatgg
     1981 gaagcgacga agagtgggaa gcagcagaaa atggattaag agaagcttta gaagagttag
     2041 gtctaccata tactattaat gagggtgatg gagcattcta tggtcctaag atagacttcc
     2101 atctaaaaga ttgtttagga agaacttggc aatgtggtac tatccaatta gatatgcagc
     2161 ttccaagaca atttgataat acttatattg gtcaagatgg tgaaaagcat agaccagtta
     2221 tgattcatag ggttgctttt ggtagtatag aaagatttat aggtatatta atagagcatt
     2281 atgctggtaa attcccagtt tggttatctc caactcaagt taagatactt cctatatctg
     2341 ataaatttat ggattatgca aatgaagtta agaaagagtt atttgataag ggtataagag
     2401 ttgaattaga tgatagagct gaaaaaattg gatttaagat aagagaagct cagcttgaaa
     2461 aagtgccata catgttaata gtaggtgaaa aagaagtagc tgataataat gtttctgtta
     2521 gatcaagaga taaaggtgaa ataggtagta ttaaattaga tgaatttata gctagtattt
     2581 caaaagaaat tgaatcaaga gaaagtatta ttcaagacta atatatatta tattagtagc
     2641 taagatattg ttatataaga taagtttaat caatatgttg atatgttgat gcatagtgat
     2701 taaatttatg aaaagatgag tataattaga ggtacagtta atcatatttt tatgattaat
     2761 gtacctctag ttttttgtat ggtagattta ttaataaata tatcatagaa gtgttttgaa
     2821 catggaaata tttatgtgca ttctttaaat aaaagtttat tttttgagtg ataattaagt
     2881 gcttgttggt aaggttatgg ctgtaaaaat cataacttta ttttcatatt tgttccattt
     2941 tcataaaaat tattatccct ttaagaaaat ttcttaatcc tcttgaaata tttccttttt
     3001 agtctaaagt taaatttctt tcttcaaaat gaatatttaa atgaactaaa atttaagtac
     3061 attttctaat cttatgagtc tatttttcat agataattgc cctataatag aagaatctat
     3121 gttggtgcta tgtggtgaca ataacagcat tgtcagtgat taattaatgc tttataaatg
     3181 attaattagt gttttatgtt atttttggac ttatcctcta tacatagatt aatttattta
     3241 actttagata gagagagaat gtcaagtaca tacacagaca ttagagttta agaaagtgtt
     3301 tatggagcaa gaacttttaa aagaggtagt gacttagttt aatttcaaga aatgataaat
     3361 acaaaagagg aaaaagagag gtgttgaaat gatagaaaaa gtagaagaaa aatctaagat
     3421 tattaaaaca tcaaaagaat ataagcgaac tataattgaa gaaaaagata aagaatggac
     3481 caaaaaatta aatgatatac ttagtagaag tagtttttct aatgatgaat tagataaaat
     3541 caaggcatta attccaaaag agattttgac taatgaaaat gttggagaag ttgtgactga
     3601 agatggatat gctaagcctg agtatgatga ggtatttaaa tcattgttca ctttaaataa
     3661 tgaatatgat ttacttgtaa attttataaa tgatattcta aaagatgctc catatgctaa
     3721 caggaatatc aaaccgttca ctcaaataaa gagaataatt agagttgaaa ctgatccaac
     3781 aattaattac attggagaga aaagacctaa acttgatatt ttggctgaag atgaagaaaa
     3841 taatcatata aatattgaga tgcaaagagc ttttgagaac gattatttgg aaagagctga
     3901 atattatctt agtcgtgtac atggtagaaa gttagaagaa ggtaaagagt ataaagaaat
     3961 tggaaaaact attggaatac atatactaaa tcatgtaaaa tataatcaca tagaagatta
     4021 tgtaaattgc ctgagattaa caatggacgg gcatccagat atttattcta gtaaaactgc
     4081 attatatttt atagagcttc caaaaattca taaatctgac tatatagtaa atagaattat
     4141 gctatgggga aaacttatta gtaatccttc taacttagat gtaagagtta tagctaagaa
     4201 tgattctatt attaaaaaag cacttgatag attaaaagaa ttaggtagca atgaagaata
     4261 tttattaaat ctgaaaagag gtgcttatat catgaataaa agtagaaatt ttaaagaaga
     4321 gattgaaaga gagacagcaa ttagagttgc aaaagaaaaa gattataaaa taattataat
     4381 gttgaaaaaa aatggtttta aattagatga catattaaat aaatttaatg attttgactt
     4441 aagtgaagat gagataaaag aagtatatga aaataattga tgataaataa aaagtgaact
     4501 tatataagtt tgctgaatcg gagtctggaa atatttatat gtatttttta atgaatgaaa
     4561 atttatttta aaaacaataa tttagaagta cagttaatta tatttttatg atactgtact
     4621 tctagttttt tctattgtag atttattatt tactaataaa tctacaatag aaatgctttt
     4681 gttgtaagaa tcattttatc taatgattgt tttttataat agttaaattt ccatttgtag
     4741 tgtttaaata taaatctata acagcatttc tataaatgtt ataaatcact ataatgattc
     4801 aactcaaagt tactgcttaa agaagcagag attttatcaa ggtatatcat ggaaaaaaat
     4861 attttattgt aatatttata agattttatt atagaaataa tgtaaaaact ttataaataa
     4921 tatattttat acctagttat agataaattt atatagttat tattaaaaaa agcaataaaa
     4981 agtttaattc aatttagcaa atttagtgat ataattaaaa aagaaaagaa atatggactt
     5041 atttggaata attttgacaa tctatttact acatgtgtag agtcgtatat aattaaatta
     5101 tacataaaat ataaaaaaat aatttagtaa tacgttagga gaaagtatta tgataaagtc
     5161 aagcttattt tggaatattt tcattgcgat atcctttatg atagaatggt ttctttgtaa
     5221 gaaaatgcta gattatacaa gtgagaaaag gatttctaac aaaaaaataa acttttgtat
     5281 ggtaggtatt attgtttttg ctgacataat atattttttt gatgtacctc caaatataag
     5341 agtaattata tctatgataa ttactattgc attttataaa atctcatatg atgtggggtt
     5401 tataaagtct gtattaatat ccttaattta ttggatgtta ctaatcgcca cagatgcttt
     5461 gagtatatct ttaacagttt ggcttaattc tataaatgac atgagtagta tggggacggg
     5521 agatatatac agaattgagt ctataatatt gggaaagata cttttgattg tgtctttgct
     5581 tttatataga gccttaaaga taaaacttaa tatagaaata aataaaagga cattatttta
     5641 tatagttatt cctatagtcg ctaatatagc atcttttttt gtaatttttg aatacatatt
     5701 taatccaggg tctggtagct taatacataa tttccaattc gttatgattt ctgttgtatt
     5761 gcttatatca agtatcactt tagttttaat tgctaaaaaa ttacagcaat ataatacttt
     5821 agttgtagaa aacaatatga ttaaaaatac tttgaagtat gaaaatgagt attatgaaga
     5881 tatagaaaag aaatatttaa aaactagatt actaagtcat gatatgaaaa atcacatatt
     5941 gtgtataaat gctatgatta aaaaaggtat tgatgttact tcttatttaa ataatttgca
     6001 ggtagaaatt cagagtaatg acttgttatt taatactggt aattttatat tagatgctat
     6061 attaagtgaa aagaaagagg tttgtgataa gaatgatata gaactaaaaa taggaataaa
     6121 ttttagttgt tgtgaagctg ttgaattagt ggatatatgt catatatttt caaatatttt
     6181 agacaatgca attgaggcat gtaataaaat tgatagtgga aatagagtta tttgtgttaa
     6241 aggtgatgtt atgcaaaatt tttatttaat aagagttgaa aatactaaga taaataagat
     6301 tattactaaa aatggagata ttttgacaga caaaaaaggt tctcttcatg gacttggaat
     6361 aaaaagtgta aaaaactctg taaataagta tggtggtaat gttgtaattg aatatgatga
     6421 taaaaaattt gtagttaaaa ttgctattcc acttagaaat gtgaagaaag cagttaatca
     6481 tgtgatttaa atgtatataa atttgatgtg tagaaattta atatataaga attaatgtat
     6541 aaaagtgatg catagaagtt taatatataa gaattaatgt ataaaagtga tgcatagaag
     6601 tttaatatat aagaactaat gtataaaagt gatgcataaa agtttaatat ataagaattt
     6661 aatatgtata aaagtgatgc ataaaagttt aatatatagg aattt
//
LOCUS       DAMJYR010000176         6584 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.181, whole genome shotgun sequence.
ACCESSION   DAMJYR010000176 DAMJYR010000000
VERSION     DAMJYR010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6584)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6584)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6584
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.181"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(619..1179)
                     /locus_tag="PC471_003468"
     CDS             complement(619..1179)
                     /locus_tag="PC471_003468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HDF2790553.1"
                     /translation="MLKKLKKMLLITCCLFMMSSTAFAETQNSEGKSFTISGYTFMYE
                     NAPQEIKDKYKENCLDIGVEPQPNDEIFITPSELKTYSNIDMYSARSSSFTVTYHYSY
                     YKVTGDKNYTVNTSTIVKYGNSGNPVHLVQLLLKKVGYALEVDSQFGNTTLSKVKSFQ
                     GSYGLTVDGIVGSATWKKFASATNLD"
     gene            complement(1225..2208)
                     /locus_tag="PC471_003469"
     CDS             complement(1225..2208)
                     /locus_tag="PC471_003469"
                     /inference="COORDINATES: protein motif:HMM:NF017390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M56"
                     /protein_id="HDF2790554.1"
                     /translation="MSNLILTDILRTSITSSLVICFLLLLRTTIFRIFNKGFNYYIWL
                     IVIVRLLFPFSYFTYTISDSKFQYKPILNEVDYSNTVLIIYLTIVILLAIYRFIKYLK
                     FKSLVIDLSYDIENLEINNLYNNLLKELNIEKHIKLKYTYEINTPSFFGLFDNYILLP
                     PHNYNLNELHWILKHELIHYKSKDLYIRYLVLFLKCVYFFNPFIYLLDRIIYHSCELH
                     CDEVVLKGCSLENIKSYANTILDSIERSSRDSNNYTTGLHRKSNFEKRVYDMFKNKGK
                     SGILIALILCLLSSFTYFKFDSIISKPINHSLDAPLPVNTGKEKSFTITID"
     gene            complement(2221..2583)
                     /locus_tag="PC471_003470"
     CDS             complement(2221..2583)
                     /locus_tag="PC471_003470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HDF2790555.1"
                     /translation="MIEKIPRAELKVMMLFWDTDKVLTSRDIVNIMSEEWKQTTTLTL
                     LSRLSKRGFLEPTKKPTHTTYKILIDKKSYLEFATSDFVKNVHNDSIVSLVDSVNKEK
                     IGKDELASLRAFIKKLKK"
     gene            3826..4473
                     /locus_tag="PC471_003471"
     CDS             3826..4473
                     /locus_tag="PC471_003471"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2790556.1"
                     /translation="MNKKLEKPFVYKREYDLTGYDVEILQKYELEQAIYVYVGSSCAY
                     NMRARSSKWRYHIRTNNKSICCNIKNFIHNLELFYKMELKLSDNIINDKLYYSNIAEF
                     EEFETLEKAREVESTIISQYQFLDSINHMLKQKIILLSNKDSVLNITKNGNTNYLKVK
                     NKYIEKHKNKPIMRYHINCQFNTDGSVKSITQEFEPILELNKKNTLSRPSRVFLK"
     gene            4748..6385
                     /locus_tag="PC471_003472"
     CDS             4748..6385
                     /locus_tag="PC471_003472"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2790557.1"
                     /translation="MEQLDSKYKLKKFLMAVFRDGIGQGNNLIDNEYVRVFQNNKSNS
                     KQLELGEEFKEYSKTTFFKNIDDIVEFTFAKNIYYENTFFNLCTTDGKAGTNENLINR
                     YALGFDFDKKELGQGFNYKDIINLFTKIGLHYHILVDSGNGFHVYVLINKTNNIKLVS
                     EVTNTLINKLGADKQANLSTQVLRVPYTYNIKNTTKQVKIIHQDKNIYRYDIEKLAKK
                     YCKDVKTVGNTNTKYILDSKLPNCIVDILKNGSKDGHKNLDLQKIVVTLRLRNKSLSQ
                     VISVAREWNYISQNSLSNSELEYQVKYMYEKLKTVNFGCTGCEFNSDCWNKIESDFIY
                     SDEDTLFNMPHKHSKDLKYKNRKGVKIMTGNQLFIYNVLLNNKDRELNIDDIMELITY
                     KRKKKVKNIVMSEKTLRETLKELQHNDYITKTKGVTKLGIKDTYNVKEVRCNIDKQYT
                     ISYFVTMAVIWGIISTEELRLYTHMRYKQDLLVKDDKIKGNILRINQEELAKDLGVTQ
                     QRISNMIESLLDTKILDVWETKINDRGFMYYTYRLNK"
BASE COUNT         2683 a          692 c          917 g         2292 t
ORIGIN      
        1 ctaattagta ggtgcttttt taaaacaagt taaaaatcaa aaatagtatt agtaagcatt
       61 ggaaatgcta gattctaaaa tagaaaagta aaaaattggt gcactatcta aacttatcta
      121 tatcgctttt tccgtcgttt ggttctctag ttacgataca ggggatatgc ttatattgag
      181 ttatagtact aatcagtgct taatatagtt aataaaatta tagttaccat agtttagtaa
      241 ctatgatgta tgttagttag aaacttgcat ttcatgagtt gtaatatctt aaagtcagta
      301 gttttgattt taaaatatta attgcactgt tacaaatgac tttattaata aaaaattagg
      361 cagcttaaaa aaagtttaga ataaatttgc taggctttgc taagctattc ttttaaaaat
      421 aactttacaa aacaaacaaa taaggactta taaatataga taaaaaaaga aaattgaaaa
      481 ggattataga gacttacaag gctttaaata tatattctta attgtaaaat tatgtaatta
      541 aaatatttac tttattataa aaagacatga taaacttatt atcatgtctt ttgttaaact
      601 tatttatatg taaaaattct aatcaaggtt tgtagcagat gcaaattttt tccatgttgc
      661 actaccaaca attccatcta ccgttaagcc ataactgcct tgaaaacttt taactttgct
      721 aagtgtagtg ttcccaaatt gactgtctac ttctaaagca tatccaactt tttttaatag
      781 caattgtact aaatgaacag gatttcctga atttccgtat tttacaatcg tagaagtgtt
      841 gacagtatag tttttatctc cagtaacttt gtagtatgaa taatgataag ttactgtaaa
      901 tgaacttgac ctagctgaat acatgtctat gttgctataa gtttttaatt cactaggggt
      961 aataaaaatt tcatcattag gttgtggctc tacacctata tccaaacaat tttccttata
     1021 tttatctttt atttcttgtg gtgcattttc atacataaat gtgtaacccg aaatagtaaa
     1081 gctttttcct tcactatttt gtgtttctgc aaaagctgtg ctactcatca taaataaaca
     1141 acaagtaatt agcaacattt tttttaattt ttttaacata attcctccct ataattttga
     1201 atatatttga ataccagcaa atatttaatc tattgttata gtaaaagatt tttctttgcc
     1261 tgtattaact ggcaatggtg catctaagga gtgattaatt ggctttgata taattgaatc
     1321 aaatttaaaa tatgtaaatg atgacaataa gcataaaatc aatgctatta aaataccact
     1381 cttaccctta tttttaaaca tgtcataaac tcttttttca aaatttgatt ttctgtgtaa
     1441 tccagtagtg tagttatttg aatctcttga acttctttca attgaatcta aaattgtgtt
     1501 agcataactt tttatatttt caagggaaca accttttaat acaacttcat cacaatgtaa
     1561 ttcacagcta tgatatataa ttctatcaag tagataaata aaagggttaa agaaataaac
     1621 acactttaaa aataaaacca aatatctaat gtataaatct ttacttttgt aatgaattaa
     1681 ttcatgtttt agaatccaat gcagttcatt taaattataa ttgtgtggtg gcaaaagtat
     1741 ataattatca aataatccaa aaaatgaggg cgtattgatt tcataagtat attttagttt
     1801 aatgtgcttt tctatattta gttcttttaa taaattatta taaagattgt ttatttcaag
     1861 attttcaatg tcatatgata agtcaataac caaactctta aactttaaat attttataaa
     1921 cctataaatt gcaagtaaaa taacaattgt tagatatatt attaatactg tatttgaata
     1981 atcaacttca ttcaaaatag gtttgtattg aaatttagaa tcagaaatag tataagtaaa
     2041 ataagagaat ggaaaaagta atctaactat tacaattagc caaatataat aattaaaacc
     2101 cttattgaaa attctgaata tagttgtcct taaaagtagc agaaaacaaa taactagact
     2161 acttgtgata ctggttctta aaatatctgt tagaattaaa ttagacatat atagtaccta
     2221 ttatttctta agttttttta taaaagctct caaagatgcc aattcatctt ttcctatctt
     2281 ttctttatta actgaatcaa ctaaacttac aatagaatca ttatgtacat tctttacaaa
     2341 atcagatgtt gcaaattcta agtagctctt tttatcaatc aaaattttat aagtagtgtg
     2401 tgtaggtttt tttgtaggct ctaaaaaccc tcttttagaa agcctagata atagtgtaag
     2461 tgttgtcgtt tgtttccatt cttcactcat tatatttaca atatctcttg atgtcaatac
     2521 cttgtcagta tcccaaaata gcatcattac tttaagttca gcacgtggta ttttttctat
     2581 cataatatgt tcctccatcc taatgttaaa aaaattaata aatgtaattt tattctattg
     2641 aaatagagta ggacagtcaa ttgttttttg attaatgatt tacatatgta gagtaaatta
     2701 aaagaagtat atcacttgaa aagttttaca tacgtagagc atttttatca aatctcttaa
     2761 tttttttaat tgaattatat atgtgaatat aagagtaaat caacttaaaa catgcaaaaa
     2821 attaatattt actgacatgc aactgacaaa tcaacaaaaa taggaattga taaaataagt
     2881 gtatatattg aaattgctat aatacataca aaaatggcat aaaatactaa tttttatgtc
     2941 cttttctact gacatgtaaa aaagtcttgt aagtattgga attactagat tacataggtt
     3001 ttagatgcaa aattgtcgtt ttgtaaaaaa ggctactaaa atttataaaa acaattatta
     3061 attgatataa attatcacaa aaaaagacat ctatttctat gaaaaaaaaa ttttcgtctg
     3121 catgtaaaaa tagctatagt aatgcgtacg aatgttctat tattacagaa ttatttgtat
     3181 gtaaattata ttgaaaatat ctatcagaat tagaatttta tatggataaa acagaactat
     3241 caaataggag tatatagtaa agatttatat aatatgatga cctcgtacaa gcctgtaaga
     3301 gccgtttaaa agttgcttta gtattaatta tctttgaaaa tttaaaatag gtttaaatgg
     3361 cttgttaaat atgatgtatt actattatgt ataggggggt agttatgtag ttacatgttg
     3421 ttttaaaaaa taaatttaac tatagttctt tcacttccac attacaaaaa ataaattgag
     3481 tgtgtatgtt tttaagtaaa aatgatgttt aaatattgaa aaatagagat atagtgtatg
     3541 taaaatttag aatagtgtat gtaattttag gcataaaaat atgtgattaa accttaaaaa
     3601 ccaggaatat agtgtatgca tttttaaaaa taaaaattgt aacctttttg taatctttta
     3661 ttaaaatact tactttaagt ctaaaaatta ggggagatgt aaggatttgg gaaaaataga
     3721 agatgttata atcataaata tggtattcgt aggcttaaag tcaaaaagga ggtgaaatat
     3781 aaatagattt ttagctaaat taagtaagaa ataggaggag atttattgaa caaaaaatta
     3841 gaaaaaccat ttgtatataa gagagagtac gatttgactg gatatgatgt tgaaatttta
     3901 caaaaatatg agttagaaca agcaatatat gtttatgttg ggagtagttg tgcatataac
     3961 atgagagcta gaagtagtaa atggagatac catataagaa caaataataa gtctatatgt
     4021 tgtaacatta aaaattttat acataacttg gaattgtttt ataaaatgga attaaagttg
     4081 tcagataata ttattaatga taagctatac tatagcaata tagcagagtt tgaagaattt
     4141 gaaacactag aaaaagctag agaggtagaa agtactatta taagtcaata tcaattttta
     4201 gattctataa atcatatgtt aaaacaaaaa ataattttat tgagtaataa ggatagtgtg
     4261 ttaaacataa ctaaaaatgg aaatacaaat tatttgaaag taaaaaataa atacatagaa
     4321 aaacataaga acaagccaat aatgagatac catatcaact gtcaattcaa tacagatgga
     4381 agtgtcaaaa gtattacaca ggagtttgaa ccaatattgg aattaaacaa aaaaaatacc
     4441 ctaagccgac caagcagagt atttttaaaa taatatttta agataacaac aaaatgagat
     4501 aatactacta gacaatgaca actcaactac caattgagtt tatggagcta ccaactccaa
     4561 tatcggtcta actgattaag tatctgtagt tatataataa tattgctatc aattttagca
     4621 tcttaacaat attattatac atactaagct aaaattattc aatagttgta aaagttgatt
     4681 agtcaataag tatatattta atgtagtgtt atctcttaaa aaaactagat aaggagataa
     4741 taaatatatg gaacaattag attcaaaata taagttgaaa aaatttctaa tggcagtatt
     4801 tagagatggt ataggacaag gaaataatct tattgataat gaatatgtta gagtatttca
     4861 aaataataaa agtaatagta aacaattaga actcggagaa gaatttaaag aatatagtaa
     4921 aacaactttt tttaaaaata tagatgatat agtagaattt accttcgcaa aaaatattta
     4981 ttatgaaaat acatttttta acctatgtac aactgatgga aaagcaggaa ccaatgaaaa
     5041 cttaataaat agatatgcat taggatttga ttttgacaaa aaagaattag gacaaggttt
     5101 taattataaa gatataatta atttatttac taagatagga ttacattatc atatcctagt
     5161 tgatagtgga aatggattcc atgtttatgt gctaattaat aaaactaata acattaagtt
     5221 agtatcagaa gttacaaata cattaataaa taaattgggt gcagataaac aagcaaattt
     5281 atctactcaa gtattaagag taccttatac atataatatt aaaaatacta ctaaacaagt
     5341 aaaaataata caccaagaca aaaatatata tagatatgac atagaaaagt tagctaaaaa
     5401 atattgcaaa gatgtaaaaa cagtaggtaa tactaataca aaatatatat tagatagtaa
     5461 gctaccaaat tgtatagtag atattttaaa aaatggtagt aaagatggac ataaaaacct
     5521 agatttgcaa aaaatagttg tgactttaag attgaggaat aaaagtttaa gtcaagtaat
     5581 atccgttgct agagaatgga actatatatc acaaaatagt ctttcaaata gtgagctaga
     5641 atatcaagtc aagtatatgt atgagaagct taaaacggtt aattttggtt gtacaggttg
     5701 tgagtttaat agtgattgtt ggaataaaat agaatcagat tttatatata gtgatgaaga
     5761 tactttgttc aatatgccac ataagcactc aaaggatttg aaatataaga ataggaaagg
     5821 ggttaaaata atgactggta atcaattgtt tatctataat gtgttactta acaataaaga
     5881 tagagaatta aacatagacg atataatgga gctgataacc tataaacgta agaagaaagt
     5941 taaaaacatt gttatgagtg aaaagacatt aagagaaaca ttaaaagaac ttcaacataa
     6001 tgattatatt acaaaaacaa aaggtgttac aaagctagga ataaaagata catacaatgt
     6061 aaaagaagtt agatgtaata tagataaaca atatactatt agttactttg ttaccatggc
     6121 agtaatttgg ggaataattt caactgaaga attaagatta tatactcata tgagatataa
     6181 gcaagattta ttggtcaaag atgataaaat aaaaggaaat atattaagaa ttaatcaaga
     6241 ggaattagca aaagatttag gagtaacaca gcaaagaatt tcaaatatga tagaatcttt
     6301 attagatact aaaattttag atgtatggga aactaaaata aatgatagag gatttatgta
     6361 ctatacatat agattaaaca agtagatttt tgataggatt agaattgatt ttctagtcct
     6421 atttttatgc aaaaaaacta attaataaaa atttcttttg gtaaaataat tgtacgagaa
     6481 ttgcaaaaaa aaaatggcat caaagtattg aaattaagcc gttttaaaaa tttcttttgg
     6541 taaaataatt gtacatatat atgtagtata tatatatatg tttt
//
LOCUS       DAMJYR010000177         6563 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.182, whole genome shotgun sequence.
ACCESSION   DAMJYR010000177 DAMJYR010000000
VERSION     DAMJYR010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6563)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6563)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6563
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.182"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(16..729)
                     /locus_tag="PC471_003473"
     CDS             complement(16..729)
                     /locus_tag="PC471_003473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2140 domain-containing protein"
                     /protein_id="HDF2790558.1"
                     /translation="MGKVAKILLSLIVIVLVLAGIVVYILTPKERYDVSSQNNKIIDE
                     EYLSKGSYINSMEVVKNPLRMVGKISVSEEEFKNLIYTLMQKHGINELENNFVEMKNG
                     KIKVAGPYKVFGLVNSQYELELRPTLKDGNLVVSLENVKIGKFKLSDKMLEKILSNYN
                     KKVPFEVNGNKITLEKSYLYPITLKNISIKKGNVDLDMEVEIDLKSQINGALGYLKDS
                     GKVQDILNHLINLKKTSVG"
     gene            1021..2685
                     /locus_tag="PC471_003474"
     CDS             1021..2685
                     /locus_tag="PC471_003474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HDF2790559.1"
                     /translation="MSNETNSSNFIKNIIINDLETGKHDSIITRFPPEPNGYLHIGHA
                     KSICLNFGLAKEFNGKANLRFDDTNPLKEDVEYVESIKEDVKWLGFDWNELNFASNYF
                     DEMYKRALILIKKGKAYVCDLTQEEMREYRGTLTEPGKESPHRNRTIEENLDLFERMK
                     NGEFKDGEKTLRAKIDMSSPNINLRDPIIYRISHSTHHNTGDKWCIYPMYAFAHPIED
                     AIEGITHSICTLEFEDQRPLYDWFVKECEMENIPRQIEFARLNINNTVMSKRKLKQLV
                     DEGIVDGWDDPRVPTISGIRRKGYTAEALRNFCSEIGVSKVNSTVDSQMLDYFLRENL
                     QPKAPLTMGVLRPLKLIITNYPEDKIEMLEIENNAKDESQGKRLVPFSRELYIEQDDF
                     MEEPVKKYFRFFPGNEVRLKGAYFVKCTDVIKDENGNVVEIHGTYDPETKSGSGFTGR
                     KVKSTIHWVDAKSAIPCEFRLFEPLILDDIPENEGKHFLKQINPNSLEILQGFVEPTQ
                     IKDAKPFDKFQFVRNGFFSIDNKYTTDEKFVFNRIVPLKSSFKPGK"
     gene            complement(2792..3691)
                     /locus_tag="PC471_003475"
     CDS             complement(2792..3691)
                     /locus_tag="PC471_003475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HDF2790560.1"
                     /translation="MKKVKLIYNPNSGEKKILSNLDTIIELYQEKNYLLIPYRLTIKE
                     PVKNAFKEVDSSYEHILIAGGDGTVDLVVNAMKELDIKIPIGILPTGTANDFSNALQI
                     SFDIKEAINEIINSKPKKIDIGKVNNKYFINVASAGMFTDVSQRINTDLKNSFGRISY
                     YIKGIEEALYMRKFKIKVSSEEMYYDGDMYLMLIFNGKTAGNINLAYKAEIDDGYLDV
                     IIFKGMPIPKSIPVLISVLRGDYLDQYNGNEILYFKTKKVHIECEDSLITDIDGEKGP
                     DFPLNIECIKDGIEVMGICSEKS"
     gene            complement(3718..4356)
                     /locus_tag="PC471_003476"
     CDS             complement(3718..4356)
                     /locus_tag="PC471_003476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003356606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein"
                     /protein_id="HDF2790561.1"
                     /translation="MDQNLNWLNEKRIEKTIKSLENNNMKGYLVSNKEELLKKIEEIV
                     KAGSLVGCGGSMTLFETGVIDFIREKYNFLDRYAKDIKPEEMKEIYRKSFSADAYFTS
                     TNAITEDGELYNLDGNGNRVAAMIYGPDKVIVIAGVNKIVKDVDEAIKRTKTIAAPAN
                     CKRLSTNTGCKTTGICIDCSSPGRICCSYTLIKRQLTPERIHVIFLNEDFGY"
     gene            complement(4693..5691)
                     /locus_tag="PC471_003477"
     CDS             complement(4693..5691)
                     /locus_tag="PC471_003477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4003 domain-containing protein"
                     /protein_id="HDF2790562.1"
                     /translation="MEQSFINGKVNLLIENYKALNEVKGSWQMGLIQHSCALAFTLKN
                     KRISPRLVEERIELIKKNTGLFSNFRGYNMFYMATLLSFESNPESSFKMILDIYKELK
                     SEKFWGDTYLPLTASIVYENREKMDYLTCISKMKIIYDYMRKKHPFLTSSDDYCNIAL
                     IAIHSKNLDEDLEYIEKCYEFLNENGFYKGNDLQALSQILLFSDDRTMLKCKKTIELK
                     KAFKENDCKMNYYGYPIIGAISLLDYREDEIIEEIKNVSNKLKEEKGFGNWSLGKSNR
                     VMISSAIVASIYADLIRNENNIGSITNNIFLNIVVAIQIACVMAATSAAIASSSSN"
     gene            complement(5789..6364)
                     /locus_tag="PC471_003478"
     CDS             complement(5789..6364)
                     /locus_tag="PC471_003478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2790563.1"
                     /translation="MKLKEALITCVVCSVCLTPLLVNGETKIDADHCKESIVNIDTIV
                     KSGKSFNSKYNQGMEDYKNSDEYKLKVQKEKLQNQLGIKIKKLVPITFEVSYYTSLNQ
                     ENGYGAITATGDDLEDGFVANNHLSFGTKIIVDGKLKTVMDRGSSKYFSNSNAIDVFV
                     PKWSNESESEYYDRVNNMGRHYKKGYIIVEE"
BASE COUNT         2226 a         1042 c          725 g         2570 t
ORIGIN      
        1 aagttttcta tatttttaac caacagatgt tttcttcaaa ttaatcagat gatttagtat
       61 atcttgaacc tttccagagt cctttaaata tcctaatgcc ccatttattt gtgattttaa
      121 atctatttct acttccatat ccaaatcaac atttcctttt tttatagata tatttttaag
      181 tgttattggg taaagataac ttttttccaa agtaatctta tttccattaa cctcaaatgg
      241 aactttttta ttgtagttac ttaatatttt ttctagcatt ttatcactta gtttaaattt
      301 tccaattttc acattctcaa gacttacaac taagtttcca tcctttaaag taggtctcaa
      361 ttcaagttca tattgactat ttaccaaacc aaatacctta tatggtcctg ctacctttat
      421 tttaccattt ttcatttcaa caaaattatt ttcaagttca ttaattccat gcttctgcat
      481 caatgtatat attaaatttt taaactcttc ctctgaaaca gatatttttc caaccattct
      541 aagtggattt tttactacct ccatagaatt tatatatgag ccttttgata aatattcttc
      601 atctatgatt ttattatttt gtgaactaac atcatatctt tcttttggtg tcaagatata
      661 tacaacaatt cctgcaagta ctaaaacaat aacaattaaa cttaacaaaa tctttgcaac
      721 ctttcccata atcgtctcct ttacaaatct gtcatcagat ttaaaataaa tttagataac
      781 taacttcaca taatattctt aacaatatac atactataat acagtctttt aaaaattaat
      841 tcaaccaatc taatgttttg taaataaact gtaaaaacat gtttttagta taaaagttaa
      901 tattttgcat aatactctta attacacctt tatttttttc taatcttgga ttaaaattaa
      961 tattatgcaa tacagtgtag tgttctgtat tataatcaaa ttaaatagga ggtatagaat
     1021 atgtcaaatg aaacgaactc atctaacttt ataaagaata ttattataaa cgacttagaa
     1081 acaggaaaac atgacagtat cataactcgt tttccacctg agccaaacgg atatttacat
     1141 attggtcatg caaaaagtat atgtcttaat tttggattag ctaaggagtt taatggaaaa
     1201 gctaatttaa gatttgatga tacaaaccca ttaaaagagg atgttgaata tgtagagtcc
     1261 ataaaagaag atgtaaagtg gttaggattt gattggaacg agttaaattt tgcatctaac
     1321 tactttgatg aaatgtataa gagagcttta atactaataa aaaagggtaa agcttatgta
     1381 tgtgatttga cacaagagga aatgagagaa tatcgtggta ctctaactga accaggaaaa
     1441 gaaagtcctc atagaaacag aactattgaa gaaaaccttg atttatttga aagaatgaaa
     1501 aatggtgaat ttaaagatgg tgaaaaaaca ttaagagcca aaatagatat gtcttctcca
     1561 aatataaacc ttagagaccc aattatatat agaatatctc attcaacaca ccataataca
     1621 ggagataaat ggtgcatata tcctatgtat gcttttgctc atccaattga agatgcaatt
     1681 gaaggaataa ctcactctat atgtacttta gagtttgaag accaaagacc tctttatgat
     1741 tggtttgtta aagaatgtga gatggaaaat atacctagac aaatagaatt tgctagactt
     1801 aatataaata atactgtcat gagtaaaagg aaattaaaac aacttgtaga tgagggtatt
     1861 gttgatggtt gggatgaccc tcgtgttcct acaatatccg gaatcagacg taagggatat
     1921 actgcagaag cattgcgcaa tttctgtagt gaaatagggg tatcaaaagt taactctacg
     1981 gtagatagtc aaatgcttga ttatttccta agagaaaact tacaaccaaa ggctcctctt
     2041 actatgggag ttttaagacc tttaaaactt attataacta attacccaga agataagatt
     2101 gagatgcttg aaatagagaa taatgctaaa gatgaatctc aaggaaagag attagttcct
     2161 ttctctagag aattgtatat agaacaagat gattttatgg aagaacctgt taagaaatat
     2221 tttagatttt tcccaggaaa tgaagtccgt ttaaaaggtg cttattttgt aaaatgtact
     2281 gatgtaatta aggatgaaaa tggaaatgta gttgaaatac atggtactta tgaccctgaa
     2341 actaagagtg gctctggctt tacaggacgt aaggtaaaat ctacaataca ttgggttgat
     2401 gctaaatcag ctataccatg tgaatttaga ttatttgaac cattaattct tgatgatata
     2461 cctgaaaatg aaggtaaaca tttcttaaaa caaataaatc ctaattcatt ggaaatatta
     2521 caaggttttg ttgaacctac acagataaaa gatgctaaac cttttgataa attccaattt
     2581 gttagaaatg gattctttag tatagataat aaatatacaa ctgatgaaaa atttgtattt
     2641 aatagaatag ttcctctaaa aagttctttt aaaccaggta aataatctta caatcaaaaa
     2701 atgaagactt aaaatataaa ttacttctac tataaataaa cttagttaga agtaattttt
     2761 tatttgagtc ttcatcattt actttaaatg attaactttt ttcactacag attcccatga
     2821 cttctattcc atcttttatg cattctatgt taagaggaaa atctggtcct ttttctccgt
     2881 caatatctgt tataagtgaa tcttcacatt ctatatgcac ctttttagtt ttaaagtata
     2941 gtatttcatt tccattatat tgatctaaat aatcacctct aagcacactt atcaaaactg
     3001 gtattgattt tggtattggc attcctttaa aaataattac atccaaatat ccatcatcta
     3061 tttcagcctt atatgctaga tttatatttc ctgctgtttt gccattaaaa atcaacataa
     3121 gatacatatc tccatcataa tacatttcct cagaagatac ttttatctta aatttcctca
     3181 tatacaatgc ttcttctatt ccctttatat aataagaaat tcttccaaat gaatttttta
     3241 agtctgtgtt tatcctttgt gatacatccg taaacatacc agcacttgca acatttataa
     3301 agtacttatt attgactttc cctatgtcta ttttctttgg ttttgaattt atgatttcat
     3361 ttatagcttc ttttatatca aatgaaattt gaagagcatt tgaaaaatca tttgcagttc
     3421 ctgtaggcaa aattccaatt ggaattttta tgtctaattc tttcattgca tttactacta
     3481 aatctacagt accatcacca ccagctatca atatgtgttc ataagaacta tcaacttcct
     3541 taaatgcatt ctttacaggt tctttaatag ttaatctata gggtatcaat aaataatttt
     3601 tttcttgata taattctatg atagtgtcta aatttgataa tatctttttt tcacctgaat
     3661 taggattgta tataagtttt acttttttca ttatttctcc ttactcattt tattctttta
     3721 gtatccaaaa tcttcattta aaaaaatcac atgtattctt tctggtgtta attgtctttt
     3781 tataagtgta taactacagc atattcttcc tggacttgaa cagtctatac atataccagt
     3841 agtcttacat ccagtatttg tacttaatct tttacaattg gctggagcag ctattgtttt
     3901 tgttcttttt atagcttcat ctacatcttt aactatttta tttactcctg ctataactat
     3961 tactttatca ggtccatata tcatagcagc aactctattc ccattcccat ctaaattgta
     4021 aagttctcca tcctctgtta tcgcattagt acttgtaaaa taagcatctg cagaaaatga
     4081 ctttctatat atttctttca tttcttctgg tttgatatct tttgcatatc tatcaagaaa
     4141 attatatttt tctcttataa aatcaatcac tcctgtttca aataaggtca tagaacctcc
     4201 acatcctact agagaacctg ctttaactat ttcttcaatt tttttaagaa gctcttcttt
     4261 gttacttact aaataccctt tcatattatt gttttccaaa gattttattg ttttttctat
     4321 tcttttttca ttaagccaat ttaaattttg atccatttta acttccccct tatttttaat
     4381 atgcacttaa ttatttataa actttatttt ctttataagt ttattttagt acatctaaaa
     4441 aaaaatacaa tataaaactt aaatagtttt acataatttt ttccaaaata acttactcaa
     4501 tcaaaaaagg aaagtagatg tactattcta ctttccctat attgtatgta gtatttattg
     4561 cctaaaatct atatattaat tatatgtttc atattatata atatcacagt taattaaaat
     4621 gtaatactat cttcttttta aacatataaa ttgtattatt aaatttaata tatccaactt
     4681 caataaatct tattaatttg atgaagaaga tgctatagct gcacttgttg ctgccattac
     4741 acatgcaatt tgaatagcaa caacaatatt taaaaatata ttattagtta tagagcctat
     4801 attattctca tttcgtatca aatctgcata aattgaagct acaatagcag aactaatcat
     4861 tactctatta ctttttccta aagaccaatt tccaaagcct ttttcttctt taagtttatt
     4921 tgaaacattt tttatctctt ctattatttc atcttctcta taatctaata atgaaattgc
     4981 tcctattatt ggatatccat aataattcat tttacaatca ttttctttaa atgctttttt
     5041 aagttcaata gtctttttac atttaagcat agttctatca tcactaaata aaagaatttg
     5101 ggataatgct tgtaaatcat ttcctttata aaaaccattt tcatttagaa attcataaca
     5161 tttttctatg tattctaaat cttcatctaa attttttgaa tgaattgcaa taagtgctat
     5221 attacaatag tcatcactag aagttagaaa tggatgtttt ttcctcatgt aatcatatat
     5281 tattttcatt tttgatatac atgtaagata atccattttc tctctatttt cataaactat
     5341 tgatgctgta agtggtaaat atgtatcgcc ccaaaacttt tcacttttaa gttctttgta
     5401 tatatctaaa atcatcttaa aactactttc tggatttgat tcaaacgaaa gtagtgttgc
     5461 catataaaac atattatatc ctctaaaatt agagaataat ccagtatttt ttttaataag
     5521 ctctattcgt tcttccacta atctaggact tattctcttg ttcttaaggg taaatgcaag
     5581 tgcacaacta tgttgtatca atcccatttg ccaagaacct ttaacttcat ttaatgcttt
     5641 gtaattttct attaataagt ttacttttcc atttataaac gattgttcca tattgccctc
     5701 ctagtaatat atctcattgt actaatagtt ttatatttat tatatatata ttaaatacta
     5761 caatcaatac atattacttc aaagaacatc attcttcaac aataatgtag ccttttttgt
     5821 aatgtcttcc catattattg actctatcat agtactctga ttcactttca ttactccatt
     5881 tgggcacaaa tacatctatt gcattagaat tactaaaata cttactagac cccctatcca
     5941 taacagtttt taattttcca tctacaataa tcttagttcc aaaacttaag tgattgtttg
     6001 ctacaaaacc atcttctaaa tcatctcctg tagcagttat cgctccatat ccattttctt
     6061 gattaagact tgtgtaataa ctaacttcaa aagttatagg tactaacttc tttattttaa
     6121 tacctaactg attttgtagt ttctcctttt gtactttcaa cttatactca tcactatttt
     6181 tataatcttc cataccttga ttatacttgg aattaaagct tttacctgac tttactatag
     6241 tatctatatt tactatagat tctttacaat gatctgcatc tatttttgtt tctccattta
     6301 ccaataatgg agttaaacat acactacata caacacatgt aatcaatgct tctttaagct
     6361 tcataaattc cccccttatt attttgatat attagcatat ttaatattat atgtctacat
     6421 ttagatttaa ataccacacc catttgcttt aagaagttgt cttatattta attcacctta
     6481 aacacttata aatcctctat cttagaatca tatagaataa agcttttatc tttaaatatt
     6541 ttttattaat cttttaaaat cta
//
LOCUS       DAMJYR010000178         6554 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.183, whole genome shotgun sequence.
ACCESSION   DAMJYR010000178 DAMJYR010000000
VERSION     DAMJYR010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6554)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6554)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6554
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.183"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            364..1638
                     /locus_tag="PC471_003479"
     CDS             364..1638
                     /locus_tag="PC471_003479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HDF2790564.1"
                     /translation="MQFMDVSKYIEGANLKVKTKSGETVKRIYFNNSATPLVLKNVVD
                     NLNCEIPLLTYINAPGIISEKNTLKYENVRSTILKLIDGDEEKDSVIYVKNATEGINF
                     LAKFFKDENPDKFVITTAMEHMANYLPFKVNFPTKVVGITEKGELDLCQLENILKNYA
                     GNVSLVTVTGASNVTGITTPIYEIARIAHKYGAKILVDIVQVIQHKPFSMMPHECDEH
                     IDFVVFSAHKCYSPLNGGALVGPKKFFEKFNPVLYGSGSTKFVNDNKILYANVPQRFE
                     AGYPEYFGTLAMGKALNTLNKIGLNRISKYERELFLYAKEEMSKIPNIIIYGDKSNNV
                     IIPNISFNISNMYYKDVAKYLVDNFGIETGAGVVGADIYVQKLLGISPKEAYVRFMRE
                     NPVGLVRISLGMYNTFDEVDSFIHAIKTLAFK"
     gene            complement(1845..2162)
                     /gene="trxA"
                     /locus_tag="PC471_003480"
     CDS             complement(1845..2162)
                     /gene="trxA"
                     /locus_tag="PC471_003480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HDF2790565.1"
                     /translation="MVKIVNNNEFINEVENKDGLVVVDFFATWCGPCKMLSPIYEALG
                     NEMVEKANFLKVDIDQSIELAQKFEVSTVPTMLIFKDGKPVDRLIGFMPKENLKNKIE
                     SYM"
     gene            2429..3181
                     /locus_tag="PC471_003481"
     CDS             2429..3181
                     /locus_tag="PC471_003481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmB family transcriptional regulator"
                     /protein_id="HDF2790566.1"
                     /translation="MDNIINELQNIGFTKYESQIYISLLKESPLTGYEISKLSGVPQS
                     KVYENITKLLNNNIIINVGTDPIKYIPINPNELLKNKKKEFDNSMDNLKNSLQILNKS
                     KSVEYVLNIKGDKNILKKAIEMIKNAKQEIIISCPYEEILELKKELLIAYKKNINIEI
                     LLLENKSLKGLDNIHIHGGNKTTLENKMLYKGIILIVDNDEILTGNLSEGSEAISIWT
                     KNSNILYIGVQYIKHEIYISEQIKGGYKNEQN"
     gene            3168..4049
                     /locus_tag="PC471_003482"
     CDS             3168..4049
                     /locus_tag="PC471_003482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HDF2790567.1"
                     /translation="MNKIKIKQVDAFTTIPFGGNPAGVVTDTSNISDEIKQNVAKEMN
                     LSETAFVSESNIADFKVQFFTPGLEVDLCGHATIGTFHALLEEGKLNTNKSIFYQETK
                     AGVLSVELKKINNENIFMMEQNTPEFENLDNCRKELAQMIGVHEDRLLEYPIIKVNTG
                     LWWLVFGLKSLDDLKNISPDLNKIKKFSGDNNIIGITPFCIETIDKKCDYHLRSIAPY
                     VGVTEDPVCGTGNACVSSYIVHHNILDKTSFIGEQGNFINRPGKVYVNITRENNNITS
                     VKIGGSAYTVLSGEIMY"
     gene            complement(4275..5630)
                     /locus_tag="PC471_003483"
     CDS             complement(4275..5630)
                     /locus_tag="PC471_003483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HDF2790568.1"
                     /translation="MSSTVKKKGKFPMGFYICSTTFSFERAAYYASKYLIYIFLTTAI
                     VHGGLGIDKGQAAIMQANLVAFTYLAPIIGGYISDRWIGARYTIPIGMLIMGVGYYLG
                     SIATTVSMVNAMIILVSIGTAFFKGNVSAVNGQLFDSQEELDTAFSVQYSFVNIGSFI
                     GTIAVGILYLKTFAKNGVLGFSQCFFIAAVLCVIGAIWFIYGWRFLGNAGKRPFKEGV
                     VAEKIEEKDKSPLTSMDKKRIWAIILISFFSVIFWVFWYLTYLAVYDYGAAFVNMNVG
                     GFDVPLAWFDSLNSLVCIVLGPVLGALWFKLASRPQGDMSLFKKTGLGLIFLGLAFLM
                     LVGAEFSRGVGAPETAKASILWIIMFGILLSLGEMLFSPLGNSFVSKYAPKKLLGVLM
                     GVWTFATFIAGKGYGYIYAFTLKFDMIKVYTIIPVILFVAAILLFLSDKTLSRLVEDD
                     K"
BASE COUNT         2521 a          943 c          825 g         2265 t
ORIGIN      
        1 attatgttct aatcaatttc tttactattt ttacaacttt attagtcata ttcataatta
       61 tttctaatta gtgtttaaca ttttatgtaa aatttataaa aaaatgtata taaattaaaa
      121 taagtgacta tactaaaaat atgatacgga taaacaataa gttcaaccta tctcccccaa
      181 aatcaagtga gctacagttt cccccaactg tagctcattt tcgtgtaatt taataaacat
      241 ctcaacaaga attaattaac aaaacccctt ataaaaacat aacatatatt aaacaagctc
      301 ttgattaaaa cttgagcttt tttaataaaa aacagaatag tttattagat aaggggtttt
      361 tttatgcaat tcatggatgt atctaaatac attgaaggtg caaaccttaa agttaaaact
      421 aaaagtggtg aaactgttaa aagaatatat tttaacaact ctgccactcc attagtttta
      481 aaaaacgtag tggataactt aaactgtgag attcctttgc ttacttatat taatgctcca
      541 ggaataatct cagaaaaaaa tactttaaaa tatgaaaatg tacgctctac tatcctcaaa
      601 cttattgatg gagacgaaga aaaggattct gttatttatg tcaaaaatgc tacagaagga
      661 ataaacttct tagcaaaatt ttttaaagat gaaaatccag ataaatttgt tattaccaca
      721 gcaatggagc atatggctaa ttacctgcct tttaaagtta attttcctac aaaagttgtt
      781 ggaattacag aaaaaggaga acttgattta tgtcaattag agaatatact taaaaattat
      841 gctggtaatg tatctctcgt tacagttaca ggagctagta atgtcacagg tataaccact
      901 cctatatatg aaatagcaag aatagctcat aagtatggtg caaagatatt ggtggatatt
      961 gtgcaagtaa ttcagcataa acctttttca atgatgccac atgaatgtga tgagcatata
     1021 gattttgttg tttttagtgc tcataaatgt tattctccac taaatggagg agctctggta
     1081 ggacctaaaa aattctttga aaaatttaat cctgtactat atggctcagg ttctaccaaa
     1141 tttgtaaatg ataacaaaat actatatgca aacgtacccc aacgttttga agcaggatac
     1201 ccagagtatt ttggaactct tgcaatggga aaagcactga acactttaaa taaaattggt
     1261 ctaaacagaa tttcaaaata cgaaagagaa ttatttttat atgctaaaga agaaatgagt
     1321 aaaataccta atataataat atatggagat aaatcaaata atgtaattat cccaaatata
     1381 tcttttaata taagtaatat gtattacaaa gatgtagcaa aatatttagt tgataacttt
     1441 ggaatagaaa ctggagctgg agtagtaggt gcagatattt atgtacaaaa attattaggt
     1501 ataagtccaa aagaagcata tgtcagattt atgagggaaa atcctgtagg tcttgttagg
     1561 ataagcttag gaatgtacaa tacttttgat gaggtggata gctttataca tgctattaaa
     1621 actttggctt ttaaataagt tttttctatt cttttttatc aataaataga gagtagtttt
     1681 gttttcacaa aaaagcaaat tatatcaaca gtcaatttca ttagcaatta gacactcatt
     1741 tgtaactata caaactattt tacactatac aaacaaaaaa ctcattacaa tgagtttttt
     1801 tgtttatata tgagatttat tttaattact tcaatttact ttaattacat atatgattca
     1861 attttgtttt ttaaattctc cttaggcata aaacctatta atctatctac aggttttcca
     1921 tccttaaata ttagcatagt tggtactgta gatacttcaa atttctgagc taattctata
     1981 ctttggtcta tgtccacctt caagaagttt gctttttcaa ccatctcatt tcctaaagct
     2041 tcatatattg gtgatagcat cttacaaggt ccacaccaag tcgcaaagaa atctacaact
     2101 actaaaccat ctttattttc tacttcattt ataaattcat tattatttac tatttttacc
     2161 ataatataca tctcctttat tatcttcttg atattaatat actacgacct atataacagt
     2221 tcaattaggc acttttttgt aaccgcctat ttttaataga tttaatatta tgataaatag
     2281 atttataaga ttaaatattt ataagactaa atatttctaa tatattttat ttttttctta
     2341 atatagttga cattttttcc cttaaattat aaaatcaaat taataactac ataggtagtt
     2401 actaatcttt tatcaaagga gtttctatat ggataatatt attaatgaat tacaaaatat
     2461 aggatttact aaatacgaat ctcaaatata catatcatta ttaaaggaaa gccctttaac
     2521 aggatatgaa ataagtaaat tatcaggtgt accacaatca aaagtctatg aaaatataac
     2581 aaagctatta aacaataata taattatcaa tgttggaact gaccctatta agtacatacc
     2641 aataaaccct aatgaattat taaaaaataa gaaaaaagaa tttgacaatt ctatggataa
     2701 tctaaagaat agcttacaga tattaaataa aagtaaaagt gtagaatacg tattaaacat
     2761 aaaaggagat aaaaatatat taaaaaaagc aatagaaatg ataaaaaatg caaaacaaga
     2821 aattataatt tcttgccctt atgaagaaat tcttgaatta aaaaaagaac tcctaattgc
     2881 ttacaaaaaa aatattaata ttgaaatact acttttagaa aacaaaagtc tgaaaggtct
     2941 tgataatatt catattcatg gaggtaataa aactacactt gaaaataaaa tgctttacaa
     3001 aggaattatt ttgattgtag ataatgatga aatattgaca ggaaacttat cagaaggcag
     3061 tgaggccatt tcaatttgga caaaaaactc taacatttta tatattggag ttcaatacat
     3121 aaaacatgaa atttatattt cagaacaaat aaaaggaggt tataaaaatg aacaaaatta
     3181 agattaaaca agttgatgct tttacaacta taccttttgg tggaaatcct gctggtgtcg
     3241 ttactgatac ctctaacatt tctgatgaaa ttaagcagaa tgtggctaaa gaaatgaatt
     3301 tatcagaaac tgcatttgtt tcagagtcaa atattgcaga ttttaaagta caatttttca
     3361 caccaggttt agaagttgat ttatgtggtc atgcaacaat aggtactttc catgcacttt
     3421 tggaagaagg gaagcttaat actaataaaa gtatatttta tcaagaaaca aaagctggtg
     3481 tattatctgt agagcttaag aaaataaata atgaaaatat atttatgatg gaacagaata
     3541 cacctgaatt tgaaaattta gataattgca gaaaagaact tgcacaaatg ataggtgtgc
     3601 atgaagatag acttcttgaa tatcctatta taaaggtaaa tactggactt tggtggcttg
     3661 tatttggtct aaaatcttta gatgatttaa aaaatatctc tccagattta aataaaatca
     3721 aaaaatttag tggtgataat aatataattg gtataactcc attttgtata gaaactatag
     3781 ataaaaagtg tgactaccat ttaagatcta tagcaccata tgttggagtt acagaagacc
     3841 ctgtatgtgg aactgggaac gcttgcgtat catcttatat agtacatcac aatattttag
     3901 ataaaactag ttttattggt gagcaaggta actttataaa cagacctgga aaggtatatg
     3961 taaatataac tagagaaaac aataacataa cttctgtaaa aattggagga agtgcttata
     4021 ctgtcttaag tggtgaaatt atgtattaat cccataccta ataaaaacaa ataaaaatac
     4081 tagctaacta aagctagtat ttttatttgt tatttttatt taataatcat actctaacaa
     4141 atattataat tatatatatc ataatattgt atttagaagt aaaattttat ttttattatt
     4201 aattagttat aaatcaatct aatacaacat aattacagta gattaaccta taaaacataa
     4261 taattgctta ctgattattt atcgtcctca acaagtctac ttaatgtctt atcagataag
     4321 aataataata tcgctgcaac aaatagtatt actggaatta tagtatatac tttaatcata
     4381 tcaaatttaa gagtaaatgc atatatatat ccatatcctt taccagctat aaaagtagca
     4441 aatgtccata ctcccataag aacacctaga agcttcttag gtgcatactt acttacaaaa
     4501 gagttaccaa gtggtgagaa taacatttca ccaagactta aaagaatacc aaacatgatt
     4561 atccatagta tactagcttt agcagtttca ggagctccaa ctcctcttga aaattctgca
     4621 ccaacaagca ttaagaatgc aagacctaag aatattaatc caagacctgt ctttttaaat
     4681 aaactcatat ctccttgtgg tctactagca agtttaaacc ataatgctcc aagaactggt
     4741 cctaatacta tacatacaag tgaatttaat gagtcaaacc aagcaagagg aacatcaaat
     4801 cctccaacat tcatgtttac aaatgctgca ccataatcat aaactgcaag ataagttaga
     4861 taccagaata cccagaaaat tacagagaag aatgatataa gtataatagc ccaaattctc
     4921 ttcttatcca tgcttgtaag aggagactta tctttttctt caattttttc agcaactaca
     4981 ccttctttaa aaggtctctt tcctgcattc ccaaggaatc tccatccata tatgaaccat
     5041 attgcaccta ttacacaaag tacagctgct ataaagaaac attgagaaaa tccaagtaca
     5101 ccattttttg caaatgtttt aaggtaaaga ataccaactg ctatagtacc aataaaagaa
     5161 cctatattaa caaaagaata ttgtactgaa aatgctgtat caagttcttc ttgactatca
     5221 aaaagctgtc catttaccgc agatacgtta cctttaaaga atgcagtacc aattgaaact
     5281 aagattatca ttgcatttac catagaaact gtagttgcaa tagaaccaag atagtatcct
     5341 acccccatga taagcatacc tataggtata gtatatctag ctcctatcca tctatctgat
     5401 atataccccc caattatagg tgcaaggtat gtgaatgcaa caaggttggc ttgcattata
     5461 gcagcttgtc ctttatctat tccaagacca ccatgaacta tagctgttgt aaggaatata
     5521 tatattaagt actttgaagc ataatatgct gctctttcaa atgagaaagt tgttgagcag
     5581 atataaaatc ccataggaaa tttacccttc ttttttactg ttgagctcat aattttaaat
     5641 cctttcttaa ttgtattttt attttttacg ttttgtccaa attctttctt ttatgaaaga
     5701 tataatatta ttattttgta aaacaatttg taattattat taagataatt gcgcatttat
     5761 ttgtagagta catattctca tggaataaaa ttaatacact tcttaatcat aaatttaaat
     5821 tatataaatt cactattttc agatatagtt ataaattaat atattctcat ctaataccat
     5881 gaaaaaacct tcgccatatg caattcttat tttttacgtg atagtatttt acaaactaaa
     5941 atcccccttt tgtagcaact tatgtaatta cattttagtg cttttttaaa ggagtctcga
     6001 ataaagcaac atatattttg ttcaaataat gtttttattt attttttaaa tttatatatt
     6061 acttcaccca accaattctc aacttaaata actaaactac tatctattca cagattaggc
     6121 ttatttctta cacattagtt tataataaac actgtgaatc taaacataaa aactgttatt
     6181 ttaaagtaat tacccatata ttcgctttga tactatttat tgaggctata ctctttatgt
     6241 ataatatatt atttataaaa tacctcaaaa tatattattc ataaaatacc tcaaactgaa
     6301 aactttttca cgaatattta tttttcacct cccctttttt atttcaccat gtaaatcgta
     6361 ccataaatat gcacttatat gcaagaaaaa aatctattaa tcgacagtaa ataattagaa
     6421 aaattcaaat tatatttatc aattatatta tataaaatat ttcctatgaa aatataaatt
     6481 aaattaatgt tcagaatagt ttttagatat gaaatcttga tttttttatt actttactta
     6541 tttgctcaaa tata
//
LOCUS       DAMJYR010000179         6493 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.184, whole genome shotgun sequence.
ACCESSION   DAMJYR010000179 DAMJYR010000000
VERSION     DAMJYR010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6493
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.184"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     misc_binding    109..320
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            483..1772
                     /locus_tag="PC471_003484"
     CDS             483..1772
                     /locus_tag="PC471_003484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HDF2790569.1"
                     /translation="MEEEKINYALEQIPENQKRGWVAMFSVLVAIGVDLSSVILGAEL
                     AQSMPMKQAILSVIVGSFFSAILYTTCSLVGSSTSLSTSMITKYVFGEAGAKIFSLVI
                     GVSLLGWFGVQVGFFAQNAQIIIKDIFNLDVSMQILSLIGGLLMMSTAIYGYKAMEKL
                     SVYSVPFLLVLMMLTIFLAFRANGISVDDNMKSTMTFAGGVSLSMSIIIVGAIVSPDI
                     SRWAKSRRDCALSSFLGIQFGNAFMIIVSIVLVKCMGTSDIMRIFITLGIAIPGIIVL
                     TLAQWTTNTSNVYSASLSIALVLKKAPEKVLTIVLGIIATLLAVFGIYEGFIGFLNLL
                     GIVIAPVGGVYTAEYYIVKQELKGFDKGVLYKPIVKRSIVSWIIGILITYLSTYGFIT
                     LTTIAPLDGFIAGFVVQSIIGKVLCSTKNKQEIDKAV"
     gene            1826..2905
                     /locus_tag="PC471_003485"
     CDS             1826..2905
                     /locus_tag="PC471_003485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF917 domain-containing protein"
                     /protein_id="HDF2790570.1"
                     /translation="MRFLTEESVDKIAVGAAVLGTGGGGDPYVGKLVAKQAIKKYGPV
                     KVISLDELDDDALVVPVSGMGSPVITIEKLLSEVELTTPLEIMEKLLNRKVDVIIPIE
                     IGGINSLMPIAVAAKKGLPILDADSMGRAFPEAQMVTFYLEGYEASPAVMSDEKGNSA
                     ILYPVDGIWSERLARTLTVEMGGSSSISDYNLSGAQVKKAAIGNTLTIAETIGGFLLE
                     NKSDSKGAVENILKELCGYKLFEGKVIDIKRELKGGFTRGHAFFAGINEYDGEYSILF
                     QNENLIAKKGDTPLCITPDLIAVLDLETGFPITTERIKYGSRVMVVAFPCNEKWRTEK
                     GIETVGPGYFGYDVEYKTVEELQGK"
     gene            2926..4470
                     /locus_tag="PC471_003486"
     CDS             2926..4470
                     /locus_tag="PC471_003486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020246462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HDF2790571.1"
                     /translation="MYRVGIDVGGTNTDACILDGELKVIHSVKVATTKDVETGVYNAL
                     KKVIDESKIDHSLIKYAMLGTTHCTNAIVERKRLNNVAMIRIGKPATTAILPFIDWPE
                     DLREKIELDSILVSGGYEFDGRKINELCKDEVVEFCNRIKGKVESVAISGVFAPVNKQ
                     QEEEVAKWVREILGDIPISLSNEIGNIGILERENGAILNSALSQVGKAVSLGFEKALK
                     DLGIDAQIYFSQNDGTLMNLEYTMKYPIFTIGCGITNSIRGASFLSKVKNAIVLDVGG
                     TTSDLGVLYNGFPRESSIPVTIGGVHTNFRMPDVLTIGLAGGTCVKGEKDNIKIGPES
                     VGYRITEDAIIFGGDTLTLSDVVTKLDMAFEDRKSNVENLDKEYCNEVYKKVILKLED
                     AVDQMKTSQDDVELVLTGGGSIIVPEKLKGISNVIRPPHYTAANAIGAALGQISGDVE
                     KVYSLDKMSREDAIEDAKKEAVNKALKAGAKEDTIEVLNIEDVPLAYLPGNALLIKVK
                     VVGDLI"
     gene            complement(4633..5175)
                     /locus_tag="PC471_003487"
     CDS             complement(4633..5175)
                     /locus_tag="PC471_003487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1697 domain-containing protein"
                     /protein_id="HDF2790572.1"
                     /translation="MKSYIALLRGINISGKNKIIMSELKASFVELGYSEVSTYLNSGN
                     VVFLSDVDDSKVISNTIRLMIKKQFELEIPVFIISQEELKDILNNAPNWWGDNNKEIY
                     DNLIFMFPDLSYDEFYDEVGNPKEEYEKVYHYKNAIFWSFSRKDYKKTNWWSKTASSN
                     VSDKITIRTANTVRKIVGMR"
     gene            complement(5323..5607)
                     /locus_tag="PC471_003488"
     CDS             complement(5323..5607)
                     /locus_tag="PC471_003488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDF2790573.1"
                     /translation="MAKNTKQTTVHIDETLLKEVKRIGINENKSISQIINESIIDYIL
                     VYRENEDKEKNEHDKNLNETINEFKKEEEKRMQAKSAARAIAEAKKRVLY"
     gene            complement(5670..6434)
                     /locus_tag="PC471_003489"
     CDS             complement(5670..6434)
                     /locus_tag="PC471_003489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HDF2790574.1"
                     /translation="MNNYLKFATYQLIGFTSSIPRLIKIKKNPDKFSLKEKFEFMQKQ
                     AKKSLDIVNIELNIIGKETLPKEPLLFVVNHSSMLDSFILTASVERPIGCVIADEPVW
                     RNIPIFKEWAKLLRCVYVNRKNNREGIKSIAQASQNILTGQSMAVFPEGDLTWIKEPN
                     SLVSEFRSGALKIAYKAKCPIVPLVIKNSKDTYEGYQPIGKINSVPVEVEFLEPIYDH
                     IENPRLKSSVLGENIKNKMINTIENFRKSNKTFKEF"
BASE COUNT         2262 a          806 c         1126 g         2299 t
ORIGIN      
        1 gaaaatttac atataaaact aaaaaatgca agtaattgac tttttaagtt gacaaaatat
       61 tgcatatata ttatgatata aatcataaat taactacaaa tcaatattat atattcgtag
      121 aagaagaaag tagcataaag gaattttaca gagagttaga tttggctgag aactaatatt
      181 ggaatttatg tgaaaaggac tttggagaat actattgtaa ttatgttaga atagtggttt
      241 aattcgcctt aagaaaatga gtgagtaaaa ttaattttta ctaataagag tggtaacacg
      301 gatttatatt cgtctcttgg atttttccaa gaggcgtttt ttattataaa cttatttcag
      361 aaaatttaaa actatatatt aaatgaaaat tataaaaata tgatagttaa taaatttata
      421 aattagaaaa ttgtaaaata tgctaaggtc agtagtttta cgatttaaat ggagggatga
      481 aaatggaaga agaaaaaatt aactatgctc tggaacagat tccagagaat caaaaacgtg
      541 gttgggtagc tatgttttca gttttagtag caataggtgt ggatttatca tctgttattt
      601 taggcgctga gttggcacag agtatgccaa tgaaacaagc aatattgtca gtaattgttg
      661 gttcattttt ttctgcaatt ttgtatacta catgctcttt ggttggttca tctacaagtt
      721 tatctacatc tatgataaca aagtatgtat ttggagaagc tggagcaaaa atattttctc
      781 tagttatagg ggtttctcta ttaggttggt ttggagttca agtaggtttc tttgctcaaa
      841 atgcacagat aataataaaa gatatattta atttagatgt cagtatgcag attttaagcc
      901 taataggtgg tctcttgatg atgagtactg caatttatgg atataaggca atggagaagt
      961 tgagtgtgta ttcagtacca tttttattag ttcttatgat gctaactata ttcttagctt
     1021 ttagagcaaa tggaatttca gttgatgata atatgaaatc tactatgaca tttgcaggtg
     1081 gagtttctct gtcaatgagt ataataatag ttggtgcaat tgtatctcct gatataagtc
     1141 gttgggctaa aagtagaaga gactgtgctc tttcaagctt tttgggaata caatttggaa
     1201 atgcttttat gataatagtt tcgatagtat tagtaaaatg tatgggaact tcagatatta
     1261 tgagaatttt tattacatta ggaattgcta taccaggtat aatagtactt acattagcac
     1321 aatggactac aaatacaagt aatgtatatt cagcatcatt aagtatagct cttgtactta
     1381 agaaggctcc tgagaaagta ttaactatag tccttggtat tatagcaaca ttattggctg
     1441 tatttggaat atatgaaggt tttataggat ttttaaattt acttggaatt gtaattgcac
     1501 ctgttggtgg tgtatataca gcagaatatt acatagtaaa gcaagaactt aaaggctttg
     1561 ataaaggagt attatataaa ccaatagtta aacgttcaat agtgtcttgg ataattggta
     1621 tattaataac ttacctttca acatatggat ttataactct tactactata gcacctttag
     1681 atggttttat agcagggttt gtagtacaat ctattattgg aaaagtgctt tgttcaacaa
     1741 aaaataagca agaaatagac aaagcagtat aaagaaattt gtttataaag tataaaataa
     1801 attagaaata tgaggagaaa gtaacatgag atttttaaca gaagaaagtg tagataaaat
     1861 agcagttggt gcagcagtgc ttggaactgg aggtggaggt gacccatatg taggtaaatt
     1921 ggttgcaaaa caagccatta aaaagtatgg tcctgtaaaa gtaataagtt tagatgaact
     1981 tgatgatgat gctttggtag ttccagtatc tggtatgggt tctcctgtaa ttacaataga
     2041 aaaattactt tcagaagtag agttaactac ccctttagaa ataatggaga aattattaaa
     2101 tagaaaagtg gatgttataa ttcctataga aataggaggg ataaactcac ttatgcctat
     2161 agcagttgct gctaaaaaag gacttcctat cttagatgca gattctatgg gtagagcttt
     2221 ccctgaagct caaatggtaa ctttttatct tgaaggatat gaggcatctc ctgccgtaat
     2281 gtctgatgaa aaaggtaatt ctgccatcct ttatcctgtg gatggaattt ggtcagaaag
     2341 attagctaga actttaactg tagaaatggg aggaagttct tctattagtg actataattt
     2401 aagtggagct caagttaaaa aagctgctat aggtaatact cttacaatag ctgaaactat
     2461 aggagggttt ttacttgaaa ataaatctga ttcaaaaggt gctgttgaga atatactaaa
     2521 agaattgtgt ggatacaagt tatttgaagg aaaagtaatt gatattaaga gagagttgaa
     2581 aggtggattt actagaggac atgccttttt tgctgggatt aatgagtatg atggagagta
     2641 ttctatatta ttccaaaatg agaatttaat tgccaaaaaa ggtgatacac cactttgtat
     2701 tacaccagat ttaatagcag tattagattt agaaactgga tttccaataa ctacagagag
     2761 aataaaatat ggctctcgtg ttatggtagt agcttttcct tgtaatgaaa aatggagaac
     2821 agaaaaagga attgagacag ttggacctgg atactttgga tatgatgtgg aatataaaac
     2881 agtagaagaa ttacaaggaa aataaaagtt taggaggaaa ttttgatgta tcgtgtagga
     2941 attgacgttg gaggaacaaa tacagatgct tgtatactgg atggggaatt aaaagtaatt
     3001 cattcagtta aggtggcaac tacaaaagat gttgaaactg gtgtttacaa tgccttaaaa
     3061 aaagttatag atgaaagtaa gattgaccat tcattaataa aatatgctat gcttggtaca
     3121 actcattgta caaatgctat tgtagaaaga aaaagactta ataatgtggc tatgataaga
     3181 ataggaaagc cggccactac tgccattctt ccatttatag attggccaga agacttaaga
     3241 gaaaaaatag aattagattc aatattggta tctggtggat atgagtttga tggcagaaaa
     3301 ataaatgaac tttgtaaaga tgaagttgta gagttttgca atagaataaa aggcaaagtg
     3361 gaaagtgttg caataagtgg agtatttgct ccagtaaata aacaacaaga agaagaagtt
     3421 gcaaaatggg ttagagaaat tttaggggat attcctatat ctttatcaaa tgaaataggt
     3481 aatataggta tattagaaag agaaaatgga gctattttaa attctgcatt gtctcaagta
     3541 ggtaaagctg tatctttagg atttgaaaaa gcacttaaag atttaggtat agatgctcaa
     3601 atatatttca gtcaaaatga tggaacatta atgaatttag agtatacaat gaaataccca
     3661 atttttacaa ttggatgtgg aataacaaat tctataagag gagcttcctt cttatcaaaa
     3721 gtaaaaaatg caatagtatt agatgttggt ggaactacaa gcgacttggg tgtactatat
     3781 aatggattcc ctagagaatc atctattcca gttacaattg gaggagtaca tacaaacttt
     3841 agaatgccag atgttttaac tattggtctt gctggtggta catgtgtaaa aggcgaaaaa
     3901 gataatataa agataggtcc tgaaagtgtg ggatatagaa taactgaaga tgcaataata
     3961 tttggtggag acactctaac tttatcagat gttgttacaa aattagatat ggcgtttgaa
     4021 gatagaaaaa gcaatgttga aaacttagat aaagagtatt gtaatgaggt atataagaag
     4081 gttattttaa aattagaaga tgctgtagac caaatgaaga ctagtcaaga tgatgttgaa
     4141 ttggttttaa ctggtggagg aagtataatt gttcctgaaa aactaaaagg cataagcaat
     4201 gttataagac ctcctcatta tactgctgca aatgctattg gtgcagcttt aggacaaata
     4261 agtggagatg tagaaaaagt atacagttta gataaaatgt cgagagaaga tgccatagaa
     4321 gatgcaaaaa aagaggctgt aaataaagct ctaaaagctg gtgctaaaga agatactata
     4381 gaagtattaa atatagaaga tgttccactt gcataccttc ctggtaatgc acttcttata
     4441 aaagtaaaag ttgttggtga tttaatttaa agaataaata taagtatttc cagaattata
     4501 taaatatttc tggactcatt tttaaactta ttttatcaat aaaaacagca taacttttga
     4561 ctatattctt ataagtttcc gaacagccga tatataccta ttttttgagg tttatcggct
     4621 gtttgttatc ttttatcgca ttccaactat ttttcttacc gtatttgctg tacgaattgt
     4681 aatcttatca cttacatttg aactggcagt ctttgaccac caatttgtct ttttataatc
     4741 tttacgacta aaagaccaaa agatagcatt tttataatga tatacttttt catactcttc
     4801 tttaggattt ccaacctcat cataaaactc gtcataagat aagtcaggaa acataaaaat
     4861 aagattatca tatatttctt tgttgttatc tccccaccaa ttaggagcat tatttagtat
     4921 atcttttaac tcttcttgtg aaataataaa aactggtatt tccaattcaa actgtttttt
     4981 tatcattaat cttattgtat ttgagataac cttgctgtca tctacatcac ttaaaaagac
     5041 aacattacca ctattaaggt atgtagaaac ttctgaataa ccaagttcta caaagcttgc
     5101 ttttaattca gacataatta tcttgttctt accacttata ttgatacctc ttaataatgc
     5161 gatataactt ttcatataat aatttcacct ctaaacattc acatttttct atgtagtaca
     5221 aacttgaatt tgcatctttt ataattcttt caaaacttct tcatatgtaa tttttttaat
     5281 ataaaaaaat cggtgcttaa tttgtaccga tttttgtttt tcctagtaaa gaactcgttt
     5341 ttttgcttct gctatagctc tagcagcact ttttgcttgc attctttttt cttcttcttt
     5401 tttaaattca tttattgttt catttagatt cttatcatgt tcattttttt ctttatcttc
     5461 gttttctctg tatactaata tataatctat tattgattcg tttattatct gagatatact
     5521 tttattttca ttaattccta ttctcttaac ttccttaagt agagtctcat caatatgaac
     5581 cgttgtttgc tttgtatttt ttgccacctt aatcacctct taaaaaaaat tctaacatta
     5641 aaattttttt tagtcaataa attttaatat taaaattctt taaatgtttt atttgatttt
     5701 ctaaaatttt ctattgtatt tatcatctta ttttttatat tttcacctag tacagaagac
     5761 tttagtctag gattttcgat atggtcataa attggttcta aaaattcaac ttcgacagga
     5821 acagagttaa ttttaccaat aggttggtat ccttcataag tatcttttga attttttatt
     5881 acgagtggaa caatagggca ttttgcttta tatgctatct ttaaagctcc acttctaaac
     5941 tccgaaacca atgaatttgg ttctttaatc catgttaaat ctccttctgg aaacactgcc
     6001 atactttgac ctgtaagtat attttgagag gcttgggcta tactctttat accctcacga
     6061 ttgttttttc tgtttacata tacacatctt agtaattttg cccattcttt aaatataggt
     6121 atatttctcc aaactggttc atctgcaatt acacatccaa ttggtctttc tacacttgct
     6181 gttagtatga agctgtcaag catactagaa tgatttacaa caaatagaag tggttctttt
     6241 ggcagtgttt ccttaccaat tatattaagc tctatattca ctatatctaa agatttttta
     6301 gcttgttttt gcataaattc aaatttttcc tttaagctaa atttatcagg attcttttta
     6361 attttaataa gcctaggtat agaagatgta aaacctataa gttgatatgt agcaaatttt
     6421 aagtaattat tcataaaatt atctccttaa aataaaatta tttattactg taaagatttt
     6481 aacataaaat aaa
//
LOCUS       DAMJYR010000180         6426 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.185, whole genome shotgun sequence.
ACCESSION   DAMJYR010000180 DAMJYR010000000
VERSION     DAMJYR010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6426)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6426)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6426
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.185"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            118..1521
                     /locus_tag="PC471_003490"
     CDS             118..1521
                     /locus_tag="PC471_003490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDF2790575.1"
                     /translation="MEKKKIGLWSLVFMNVSALYGIRWIAKSTASSFGLGLGAIPMWL
                     LFSFIYFVPGALICAELASTYQKGDGGLYDWVKEAFGEKYGFLVSWLNWTAKIFWYSS
                     FLTFLAVNVSYTIGNPALADNKMFVLILSLIIFWALSLIATKGISFTKIFTNTGALGS
                     TIPSAILIIFSFVAVFVLKKHDIASTYTIASMTPKLNMDAFVAISAIMFGLAGAETAA
                     NFITEIDKPEKNFPKAILISAGIVASLYVLGSIAITMIIPPDQITASKGVLDALSAVC
                     ASLGIGSWLVQLIAFGIAFSVLGAIVLYIASPIKMLFGSVKKGIFPDSLVEVNEHKIP
                     SKAVILQAIIVTIILLVTTLMPSVDAIYNVLVTMTALTALFPYVLLYASYIKLRKERP
                     DEIRPYTMAKSTSTCLGLAKMVLVVTVVGILLSAAPVMPTFAENVIYEIEMIGGGLLV
                     ILSGLWLWNRYEKKTAK"
     misc_binding    1612..1852
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1895..2593
                     /locus_tag="PC471_003491"
     CDS             1895..2593
                     /locus_tag="PC471_003491"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HDF2790576.1"
                     /translation="MNVIGIIGAMDEEVSILVDLMDIRETIKKASLEFYKGILEGKNV
                     VLVKCGIGKVNSALCAQILISEFKVDAIVNTGVAGALNEKLDVNDIVISTDAIQYDVD
                     TTAFGDPKGVIPRMKTSVFKADERLIDAAYKSSVEEVKTHKVLKGRVVTGDKFINSKE
                     LKEELVNDFGGYCGEMEGGAIAHVCYLNNTPFVIIRAMSDKADGSADVTYDVFVHDAA
                     NNSKDIVLNMLKSI"
     gene            2616..3617
                     /gene="trpS"
                     /locus_tag="PC471_003492"
     CDS             2616..3617
                     /gene="trpS"
                     /locus_tag="PC471_003492"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HDF2790577.1"
                     /translation="MSEKKVIFSGAQPSGKMTLGNYLGAIKNWTELQDNYDCYYSIVD
                     LHAITVPQDPKVLRANTIELLAQYIACGLDPEKNTIFIQSHVKEHVELMWVLNTMTYM
                     GELSRMTQFKDKSQKSEANLNAGLFTYPVLMAADILLYQTDLVPVGDDQKQHLELARD
                     LANRFNNRFSPTFVVPEGYYPKGGARVMSLQEPTKKMSKSDSNENAFILLADDSDSIK
                     RKIKRSVTDSLGVVKYNDEQPGIKNLIDIYSNLSKKSVEEIVNMYEGKGYGIFKEDVA
                     EVVSEALRPIREKYVDLLNNKDYLEKIYSMGAEKAEKQARKTLRKVYKKVGFIERRY"
     gene            complement(4269..5836)
                     /locus_tag="PC471_003493"
                     /pseudo
     CDS             complement(4269..5836)
                     /locus_tag="PC471_003493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021426623.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="iota toxin protein Ib"
     gene            complement(6172..6354)
                     /locus_tag="PC471_003494"
     CDS             complement(6172..6354)
                     /locus_tag="PC471_003494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2790578.1"
                     /translation="MKKFRKHKSISNCISILLILYLTLGSLLPNNIYAQDLQSYSGKV
                     CNTTYKAPIERSEGFF"
BASE COUNT         2275 a          770 c          992 g         2389 t
ORIGIN      
        1 aactgttttt gaatatgcaa atattttgaa aaatatcagt ttaataataa tgaatctatt
       61 ttattaattt tgaaattaaa tttaaaataa ttaatagaga ggggaaatta aattaggatg
      121 gaaaagaaaa aaataggcct atggagtttg gtatttatga atgtatcagc tttatatggt
      181 ataagatgga tagctaaatc tacagcatca agctttggtt taggattagg tgcaatacct
      241 atgtggttat tattctcatt tatatacttt gtaccaggag ctttaatttg tgctgaatta
      301 gcttctacat atcaaaaagg tgatggaggt ctttacgatt gggttaagga agcctttgga
      361 gaaaagtatg gatttttagt ttcttggtta aactggacag ctaaaatatt ttggtattct
      421 tcatttttaa ctttcttagc agtaaatgtt tcttatacaa taggaaatcc agcccttgct
      481 gataataaga tgtttgtact tatattatct ttaataatat tctgggcatt atctcttata
      541 gcaactaaag gaataagttt tacaaaaata tttactaata caggtgcatt aggttcaaca
      601 ataccaagtg ctatacttat aatattttca tttgtagcag tatttgtgtt gaaaaaacat
      661 gatatagctt ctacatacac aatagcttca atgactccaa aacttaatat ggatgcattt
      721 gttgctatat cagctataat gtttggttta gcaggagcag agactgctgc taacttcata
      781 acagaaatag ataagcctga aaagaacttc cctaaggcaa tattaatatc agctggaata
      841 gtagcttcat tatatgtatt aggttcaatt gcaataacaa tgataatacc accagaccaa
      901 attactgctt ctaaaggtgt attagatgca ctttctgctg tttgtgcgtc tttaggaatt
      961 ggttcttggt tggttcaatt aatagctttt ggtatagctt tttcagtatt aggagcaata
     1021 gttctttaca tagcatcacc aataaaaatg ttatttggta gtgttaaaaa aggaatattt
     1081 ccagatagtt tagtagaggt aaatgaacac aagatacctt ctaaagcagt tatacttcaa
     1141 gcaataatag ttactataat actattggtg actacattaa tgccttctgt tgatgctata
     1201 tacaatgttc ttgtaactat gacagcatta actgcattat tcccatatgt attgttatat
     1261 gcttcttata ttaaactaag aaaagagaga ccagatgaaa ttagacctta tactatggca
     1321 aaatcaacaa gtacttgcct aggtttagca aaaatggttt tagttgtaac tgtagtgggt
     1381 atattattat ctgcagcacc agttatgcct acatttgctg aaaatgttat atatgagata
     1441 gagatgattg gtggaggtct tctagttata ttatctggtc tatggttatg gaatagatat
     1501 gaaaagaaaa cagctaaata aaatgtattt taaaaatttc ttttggatat tataatatat
     1561 agttgacaat ttatagtgta aggaatatta taaaataaag aaattaataa attaaatcct
     1621 gagataagaa atagtaaata aatatatttt tgcacagaga attaaggatg ctgagaactt
     1681 aataaaaagt atttattgaa tgggtctttg aggagtaaaa tgaaatgtaa tttaatatta
     1741 cagagtattt gatatcgtgt atgcacgtta tagcatatat taagatatat atattcaatt
     1801 ttgagtgtat ataaatttag gtggtaccac ggaaaatata tccgtcctat tgatttagga
     1861 cggatttttt aatatagaaa atttaggagg aaaaatgaac gtaataggaa ttataggtgc
     1921 aatggatgaa gaagttagta ttttagttga tttaatggat attagagaga ctataaaaaa
     1981 agctagctta gaattttata agggaatttt ggaaggtaag aatgtagtgt tagtaaagtg
     2041 tggcataggt aaggttaact ctgctctttg tgcccagata ctaataagcg aatttaaggt
     2101 tgatgctatt gttaatacag gtgttgcagg agctttaaac gaaaaacttg atgttaatga
     2161 tatagtaata tcaactgatg ctatacagta tgatgtagac actacagcat ttggagaccc
     2221 taagggagtt ataccaagaa tgaagacttc tgtatttaaa gcagatgaaa gacttataga
     2281 tgctgcttac aaatcttctg tggaagaagt aaagactcat aaagtattaa aaggaagagt
     2341 agttacagga gataaattta taaattctaa agaattaaaa gaagaattag taaatgattt
     2401 tggtggatat tgtggagaaa tggaaggtgg agctatagct catgtatgct atttaaataa
     2461 tactccattt gtaataataa gagcaatgtc tgataaggca gatggaagtg ctgatgttac
     2521 ttatgatgta tttgtacatg atgctgccaa taactctaaa gacatagttt taaatatgtt
     2581 aaaatcaata taaatatata aggaggtaca aaaaaatgag tgaaaaaaaa gttatattta
     2641 gtggtgcaca gccatcaggc aaaatgactt taggaaatta tttaggagct ataaaaaact
     2701 ggactgaact tcaagacaat tatgattgtt attatagtat agttgatttg catgcaataa
     2761 cagtacctca agaccctaaa gttttaagag ccaatactat agaattactt gctcaatata
     2821 tagcttgtgg attagaccca gaaaaaaata ctatatttat acaatcacat gtcaaagagc
     2881 atgttgaact tatgtgggta ttgaatacta tgacttatat gggtgaatta agtagaatga
     2941 ctcaattcaa agataaatca caaaagagtg aagcaaatct aaatgcaggt ttattcacat
     3001 atccagttct tatggctgct gacatattgt tgtatcaaac tgacctagtt ccagttggag
     3061 atgaccaaaa acaacattta gaattagctc gtgatttagc aaatagattt aataatagat
     3121 tttcacctac ttttgtagtt ccagaaggat actacccaaa aggtggagca agagtgatga
     3181 gtttgcaaga gccaactaaa aaaatgagta aatccgattc aaatgaaaat gcatttatac
     3241 tattagcaga tgattcagat tctataaaga gaaagataaa aagaagtgtt actgattctt
     3301 taggtgttgt aaaatacaat gatgaacaac caggtattaa aaatctaatt gatatatatt
     3361 ctaatttaag taaaaagagt gtagaagaga ttgtaaacat gtatgaagga aagggttatg
     3421 gcatatttaa agaagatgtg gctgaagtag tatcagaagc tttaagacct ataagagaaa
     3481 aatatgttga ccttcttaat aataaagatt atttagaaaa aatatatagt atgggagcag
     3541 aaaaagccga aaaacaagct agaaaaactc ttagaaaagt ttataaaaaa gttggattta
     3601 tagagagaag atactaatta ttagctatct ttacttgata aataaatttt aaagttatta
     3661 aaaaggtata tgatatgaaa gagagagtct caaagtgaac ttttgttcat gaggcactct
     3721 tttttgtatg aaataaaata tattttaatc atttcaacaa tgaaaaagag gattatctct
     3781 attttgagac agcatctttt gataataaaa aaagatgagc ttttgctcat ctcaaagggg
     3841 ttagattatt cttactttgg ggaaagtaga ataaaggtaa aagttaactg atgtcaactt
     3901 tgtctttttt gcatatcact actgagcata aaaaaatcag aaaaccatag ccttctgaaa
     3961 caacttctaa catacattgt attttatacc caatatgtaa tttatgagtt aattttaact
     4021 cttataatga aaattgtcaa taatatatca aaaaaatatt ttttaaattt atataattaa
     4081 atgaatttat gtttatatat aggtgatatg atacagataa ttatcttttt aatacaatat
     4141 agttctaaaa aagaatatat tagaagaaga taatatttaa ataaaaaata ccttataaag
     4201 ttaatttttt aatactttat aaggtaaata catattgtat tgtggggata aatttaaatc
     4261 atttgatact aatcaacact aagaaccaat aactctctat catcttgagt aattgtatat
     4321 attcttaatt tcttgtatgt cataatattt tctccacctg taaaataact cctaagatta
     4381 acacaatcag ttttaggttg atttgtatct cctaaataat tacgcaattc tttatcttta
     4441 gtttcaatat ctttaaatcc aaaatattgt aaagtagctt caactctata tttttggaca
     4501 taatcgagag cttctttatt agtttatgtt ggtgtaaaat acataccatt tatataagcc
     4561 tttattgtac ttggattaaa tttaaaatct gcagaatata ttttatgtgt aactattatt
     4621 tcttgaacag ttggaatttt aacttctggt tcatcaagta tttcaggagt actatttagc
     4681 tctgtaatag ataagttatc taagtatatt gtatcactat caattaatgc tatatttata
     4741 ttagaagaat ctttttcagt agtttcaaat tcataactaa attttgtata tccttgttct
     4801 agtaccaaat aatccgtttt ttcttctttt acttttatat ttacaattat tgaattatat
     4861 gttaaaggat tttttgaatt ttactaaaaa cataacgttt ataaggtttt agttcagaca
     4921 taggtatttt aatcttcgtc tcaccattta atctatttgc tgagcctttt aaaccatctt
     4981 gatttgtagt attaacatta ctccatatac tagggtaatt attgtaatca tcaaaattag
     5041 taaagtaagt tggtgttttt ataagtatat tcattcctct ttcaagttta acattacata
     5101 tttgtttatc agacaaagtt tttaaactgt ctttaatctt attgcctata ttatcatcaa
     5161 atatgagttc aacacaactt tcatctattg gtatattatt aaaatataac aaaccatctt
     5221 ttttagtagt gccaaaagtt ttttcaattg cttctccaat tgtaagttca ggtgttttat
     5281 cttctgggac ctgtaaactt ttagcagtaa ttcttctttt gtaagattca ttttctgtat
     5341 ctaatataat agatacagaa atactgtcaa tttgacctat atcgtcttac caactatttc
     5401 attctgttac tatttgtcca gaaccatttt ttgtaccaaa atttccactt acttgtgttg
     5461 tttctaattt aatttgcttt ccatcatcta atttttttaa ttaattataa tttataggaa
     5521 tcagtctaga actaaattga tacattgtgt taagagccat aggtgaaaat ccttttttga
     5581 gataagtatt tccaggagat agattattgc caatttgatt ttcttgtgct ttgatagttg
     5641 ataatgcatc tccatctaac actaaatttg ttgttggtgt cactttgtac ataggtgcag
     5701 tacctgtgtt gtagtatcta atatttgcat ttatatatgc agattctcct ttgtttatac
     5761 ttaatccagt atttcatgat tatccattgc tattttgaac agcagttgaa ttatctgttg
     5821 tatggtaata atttgtcttg tatagtttct ttaaactcat taaatttttc taatgaaatt
     5881 tcgttttgat tttttgttct tattacttta ttaaatgcaa atttttctgg agattcaaaa
     5941 taatatacat acataggttt atctatttta ttttttgata tagcatttcg aagttctcta
     6001 tattcttttt ctcaagaatt tgcttctatt tgataatcat aaaaataatt tcttgtctga
     6061 gaatatttgc ttatctctat agaatctttt ttatatgatc ctaatacttc tttttcagat
     6121 ttttcaagtt tttgatcttt tctttctcat tctttagcct tttctttatc tttaaaaaaa
     6181 tccttctgat ctttctatag gagccttgta agtagtatta caaacttttc cactatagct
     6241 ttgtaaatct tgtgcataaa tgttattagg taacaaacta cctaaagtta gatataatat
     6301 caacaatata gatatacaat tactaatact tttatgtttc ctaaattttt tcatttattc
     6361 tccctacaaa tattagttta gttaactttt gaattttaat ttatttagat tatataatct
     6421 aaataa
//
LOCUS       DAMJYR010000181         6355 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.186, whole genome shotgun sequence.
ACCESSION   DAMJYR010000181 DAMJYR010000000
VERSION     DAMJYR010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6355
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.186"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            59..697
                     /locus_tag="PC471_003495"
     CDS             59..697
                     /locus_tag="PC471_003495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDF2790579.1"
                     /translation="MSELSKRDKEKIQRENEIIDKAEKLFCLNGFDNTTMNELAKEVE
                     YTKRTIYKYFSCKEDLFFAVVLRGYKRLWDNVKIESAKGKTGFEKIKLSYFAFHKFYS
                     VEPSLLCLMGMIGIVNSEKSDTDMLFKEKFFSFNKFMFDEIQGMFEIGKNDRSIRHDI
                     EIPTLMYSSIFTLTGFFNLLSVTGKSYLNNFNIDEEKFIETTLALLIDSIKA"
     gene            829..1491
                     /locus_tag="PC471_003496"
     CDS             829..1491
                     /locus_tag="PC471_003496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2790580.1"
                     /translation="MAKILLLEDNDTITFGIKTSLEKKNHTVCCYNTIAEAKKNFDKT
                     IDLILLDLNLPDGIGYEFCQYVKSIEDTPVIFLTVRDDEKDIIRGLDMGADDYVVKPF
                     LLSVLQSRINAVLRRTHSNTNNTITCENISLNKVEVKAYLDNVEVPLTAGEYKLLLSL
                     MENKNRAMTRTKLLEMLWDVDGNFVNDNTLTVTIKRLREKLKNPPCIKTLRGIGYRME
                     DN"
     gene            1491..2672
                     /locus_tag="PC471_003497"
     CDS             1491..2672
                     /locus_tag="PC471_003497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF2790581.1"
                     /translation="MKTPRNINSTVVFIIFCLIVTFLLSVILTTITSFNEYNVLSKAL
                     GASYEYNPKSTESILKSLKNSSDKDFKTGNEILSKHGYSKSTFWSKNFFYFLVISFGI
                     ISTMATVVYIIKHRTNKQKLSRINNLTQYLEEVNLGKESVLLRCEDEFSHLEDEIYKT
                     VGELRCSKEFALKERQTLSDNLADIAHQLKTPITSMSLMAQLLSENCPDEEIDYINRL
                     TNQISRLERLVSSLLTLSKLDASTLIFESKLIDVHSLLTYAIEPIEGSLREKHQTFTI
                     EASPSVEFEIDINWTLEALLNILKNCSEHIEDGGYIVAYYSQNPLYVEIIIEDNGKGF
                     AQEELPYIFNRFYRGKNASKDSIGIGLALSKSIIERQNGTLHAENCHNGGARFIIKFY
                     T"
     gene            complement(2853..5513)
                     /locus_tag="PC471_003498"
     CDS             complement(2853..5513)
                     /locus_tag="PC471_003498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2790582.1"
                     /translation="MNILQEYTLDFVRRNKRSSIAIMLAILLTTTMMSSLCGLLYTMW
                     TDFIKLSIEKEGNWHGELFDYTYGSDLPLIENFSSVDDVMVKGQWYVGKINDEKRDYI
                     IYRNATSEYWNSMPEKDTIIEGRRPNNANEIALSKQYFENNPSVKIGDKITLPIGDRI
                     LNGKPLEPIAPKSKGESFKKISEATLTIVGKVDVSTSSVIPAYTALGYLDKTSIKPND
                     SITVYLRFKNIRDTYKELPKLAKSLGWKTNEYGKYNLKYNSNYLLKMLVFSPEQKASM
                     SSISKFSTPIMYLTIAIFTVAVFVMVIYNAFSLSANARLTQLGILSSVGASPKQIKRS
                     VVFEGFLLTIIPLPIGLFLGWLLCNRLIVYVNSVNYHTDVPEVVFTYGIPAFLPAVLL
                     TIVTVWISALIPARKVSKISPIEAIRQGDSVKIKKSHKYSLGKIFGIEGELASNALRA
                     RKKSYRTATISLTLSFLLLTCFLHIIANQEGAKAVYGSDNYKDQRDIAVYLENSEPVE
                     SAFIEKLDNTEGVKSSLYYQKMSASTWLTEKDASKELAKNGGFKNIASSKKYSPIERD
                     GKFRIISNIIGLDDKSFTEYCRQISVDPKLFYDTKNPTSIVYNKEEDINRSTRRNKVY
                     IDYLNLSIGDNIKLNEKVYDEDKGDYTYNMKVGAITSTLPKIVDTSSKYTLMQIVPMS
                     MVQEISKNYDEVSRLRSNRLIGVFKVNNREDIPRVREKLESIYNKDYGSGDYSIEDVL
                     ESEKQKASGIKTMNLIVGFITGLLAMIGLSNVLATVSGNIRSRRQEFAMLRSVGLSPE
                     GIKKMLILEGLFLGITPLLLSIPVQIGIVYAFLRINEIYFIEYLPFAPISTIIGFTIL
                     ILFIVIASYMTGYKQLKNENIVESIKNETI"
     gene            complement(5510..6193)
                     /locus_tag="PC471_003499"
     CDS             complement(5510..6193)
                     /locus_tag="PC471_003499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2790583.1"
                     /translation="MEILKCENLTKIYGSNQTRVTALNNVNLSVQKGDFVSIVGASGS
                     GKSTLLHLLGGVDRPTSGKIYVEDTEISSLKEEALAVFRRRKVGLIYQFYNLIPTLDV
                     RKNILLPMLLDKRKVDEDRFSEIVSILGLSDRLNHLPSQLSGGQQQRVSIARSLIYRP
                     AILLADEPTGNLDRKNSEEIVDLLNLSNKRFNQTILLITHDEKIALEANRIVTMEDGV
                     IVSEKVVKK"
BASE COUNT         2231 a          976 c          874 g         2274 t
ORIGIN      
        1 atatttacat aaatatacca tcagtatact tttttatcaa tagaaaggaa taatttatat
       61 gtcagaatta tcaaaaagag ataaagaaaa aatacaaaga gaaaatgaga ttattgataa
      121 ggcagaaaaa ttattttgtc ttaatggatt tgataataca actatgaatg aattagcaaa
      181 agaagttgaa tatactaaac gcaccatata caagtacttt tcatgtaaag aagatttatt
      241 ctttgctgtt gtcttaagag gatataaaag actttgggat aatgttaaaa tagaaagtgc
      301 aaaaggcaaa acagggtttg aaaaaataaa actttcatac tttgcttttc ataaatttta
      361 ctctgtagaa ccttctctat tatgccttat gggaatgatt ggtattgtaa actctgaaaa
      421 atcggatact gatatgctgt ttaaagaaaa gtttttttct tttaataaat ttatgtttga
      481 tgaaatacaa ggaatgtttg aaattggaaa aaatgataga agtattaggc atgatattga
      541 aatacctact cttatgtatt catcaatatt tacacttaca ggatttttta atttactatc
      601 tgtaacagga aaatcatacc tgaacaactt taatatagat gaggaaaaat ttattgaaac
      661 aactcttgct cttttaatag attcaattaa agcataaacc taagtttata aattaaagtt
      721 tatatactta aatgataatt ttttctatct ctaatatgaa aaatattaaa cataaaacta
      781 ataacttagt taatggaata actaaattac tgaaaggatt aaaagtaaat ggctaaaata
      841 cttcttttag aagataatga tacaattaca tttggaataa aaacttccct tgaaaagaaa
      901 aatcacactg tatgttgtta taatacgatt gctgaagcta aaaaaaattt tgataaaact
      961 attgatttaa ttctgcttga tttaaatcta cctgatggta ttggatacga gttttgccaa
     1021 tatgttaaaa gtatagaaga tactccagtg atatttttga cagtccgtga tgatgaaaaa
     1081 gacattatac gtggattaga tatgggtgca gatgactatg tggttaaacc gtttttgctc
     1141 tcagtacttc aatccagaat taatgcagtt cttagaagaa ctcattcaaa tactaacaat
     1201 actattacat gtgaaaatat aagcttgaat aaggttgaag tcaaagcata tttagataat
     1261 gttgaagtac ctttgacagc aggagaatat aaacttcttc taagtcttat ggaaaataaa
     1321 aatagagcta tgactcgtac aaagttacta gaaatgctct gggatgtaga tggtaatttc
     1381 gtaaatgata acactcttac tgttacaata aaacgtctta gagaaaaatt aaaaaatcca
     1441 ccttgtataa aaacattaag aggcatagga tatagaatgg aggacaacta gtgaaaactc
     1501 caagaaatat caacagtaca gttgttttta taatattctg cttaattgta acttttttat
     1561 tgagtgtaat tctaacaact ataacttctt ttaatgaata caatgtactg tctaaggctc
     1621 ttggtgcatc atatgagtac aatcctaagt ctacagaaag tatactaaaa tcactgaaaa
     1681 attctagtga taaggatttt aaaacaggaa atgaaatttt atcaaaacat ggatattcta
     1741 aaagtacatt ttggagtaaa aacttttttt attttctagt gatatcgttt ggtataattt
     1801 caacaatggc tacagttgta tatataatca aacatagaac aaataagcaa aaactgagta
     1861 gaataaacaa tcttactcaa tacttagaag aagtgaattt aggaaaagag tctgtacttt
     1921 tacgatgtga agatgaattt tctcaccttg aagatgagat atataaaaca gttggggaac
     1981 ttcgttgttc aaaagaattt gctttaaaag aaagacaaac tctttcagat aaccttgcag
     2041 atattgctca tcaacttaag actccaatta cctctatgtc acttatggca caattacttt
     2101 cagaaaattg tccagatgaa gaaatagatt atattaacag acttacaaat caaatctcaa
     2161 gacttgagag actagtttca tctttactta cattatcaaa attggatgca agtactttaa
     2221 tatttgaaag caaacttata gatgtacatt ctcttcttac ttatgcaatt gagcctatag
     2281 aaggttcttt aagagaaaaa catcagacat ttactatcga ggcttctcct tcggtagaat
     2341 ttgaaatcga tattaattgg actttagaag ctcttttaaa tattcttaaa aattgtagtg
     2401 agcatataga agatggtgga tatatagttg cttattatag tcaaaatcct ctatatgttg
     2461 agattatcat agaagataat ggtaaggggt ttgctcagga agaacttcca tatattttta
     2521 atagatttta tcgtggtaaa aatgctagta aggacagtat tggtataggg cttgctctat
     2581 caaagtctat tatagaacgt caaaatggta ctctacatgc agaaaattgc cataatggtg
     2641 gtgcaagatt cataattaaa ttttacacat aaaatgctgg attataggta aaatacctat
     2701 aatccagcta tatctatatt ctatgttatc tattgtttta gtaatatact tctatttaca
     2761 tttaaggttt tattaaacaa acaacttaac tatccctata cttgtaagta gtcttaaatc
     2821 gaaaccttta aataagttca aatagatttt gtttatattg tttcattttt tatagattct
     2881 actatatttt cattcttcaa ctgcttgtat cctgtcatat atgatgctat tacaataaaa
     2941 agtatcaata tagtaaaacc tattattgta gatataggtg caaatggtaa atactctata
     3001 aaatatattt catttatacg tagaaaagca tacactattc ctatttgcac aggaatacta
     3061 agtagtaatg gtgtaattcc taagaataaa ccttccaaaa taagcatctt ttttatgcct
     3121 tctggagata atccaacaga acgcaacata gcaaactctt gtcgtctact tctaatattt
     3181 cctgacactg tagcaagtac atttgataat ccaatcatag ccaataaacc tgtaataaag
     3241 cctactatta aattcatggt ctttattcca ctagcctttt gtttttcact ttctaaaaca
     3301 tcctcaatac tataatctcc tgaaccatag tctttattat aaatggactc taatttttct
     3361 cttactctag gaatgtcctc acgattattt actttaaata ctcctatcag cctatttgaa
     3421 cgtaacctac ttacttcatc ataattttta gatatttcct gcaccataga cattggaact
     3481 atttgcatta atgtatactt tgaactagta tctactattt taggaagtgt gcttgtaata
     3541 gctcccactt tcatgttgta agtataatcc cctttatctt cgtcatatac tttttcattt
     3601 aatttaatgt tatctcctat tgaaagattt aaataatcta tatatacttt atttctacgt
     3661 gtactacgat taatatcttc ctccttatta tatacaattg aggttggatt ttttgtatca
     3721 taaaataact ttgggtctac agaaatttgc ctacaatatt ctgtaaaact cttatcatca
     3781 agcccaatta tatttgaaat tatacgaaac ttgccatcac gttctatagg agagtacttt
     3841 ttagatgaag ctatattttt aaatcctcca tttttcgcaa gttcttttga agcatctttt
     3901 tcagtcaacc aagtggaagc agacattttt tgataatata gtgaactttt tacaccttct
     3961 gtattatcta atttttcaat gaaagcagac tctacaggtt cactattttc caaataaact
     4021 gctatatctc tttggtcctt ataattatca cttccataaa cagcttttgc tccttcttga
     4081 tttgcaatta tatgcaaaaa acatgtaagt aataaaaaag ataatgttaa agatattgtt
     4141 gcagtacgat aagatttttt acgagcacgt agtgcgtttg atgcaagttc tccttctatt
     4201 ccaaatatct ttcctaatga atatttatgc gactttttga ttttaacact atctccttgt
     4261 cgtattgctt ctataggaga tatctttgat acttttcgtg ctggtataag tgcagaaatc
     4321 cagactgtta ctattgtaag taaaactgct ggcagaaaag caggaatacc ataagtaaat
     4381 accacttctg gaacatcagt atgataatta actgaattaa cataaactat aagcctatta
     4441 cacaataacc atcctaaaaa taaacctatt ggtaatggta taattgtcag taaaaagcct
     4501 tcaaacacta cagacctttt aatttgtttt ggtgatgcac caacactaga taatatccct
     4561 aattgagtaa gtctagcatt tgcagacagt gaaaacgcgt tgtaaatcac cattacaaac
     4621 acggctactg taaaaattgc tattgtaaga tacataatag gagttgaaaa ttttgatata
     4681 ctactcatag atgctttctg ttctggtgaa aagactaaca tctttaaaag ataattacta
     4741 ttgtatttaa gattgtattt tccatattca ttagtcttcc atcccaatga tttagcaagt
     4801 tttggaagtt ccttatacgt gtctcttata tttttaaatc gcaagtacac agttatacta
     4861 tcattaggtt ttatacttgt tttatcaaga tatccaagag ctgtatatgc aggtattact
     4921 gatgaagtag aaacatctac ctttcctact attgtcaatg tagcctctga aattttttta
     4981 aaactttccc ctttcgattt tggggctatg ggttcaagag gctttccatt taggattcta
     5041 tctcctattg ggagagtaat tttatcacca atttttacac ttggattatt ctcaaaatat
     5101 tgttttgata atgcaatttc attagcatta tttggtcgac gaccctctat tatagtatcc
     5161 ttttctggca tagaattcca atactctgat gtagcatttc tataaattat ataatctctt
     5221 ttctcatcat taattttgcc tacataccat tgtcctttta ccattacatc atcaactgaa
     5281 gaaaaatttt caattaatgg taagtcactt ccataagtat agtcaaacaa ttcaccatgc
     5341 cagttacctt ctttctcaat tgaaagtttt ataaaatctg tccacatggt atacaacagt
     5401 ccacacaaag aagacatcat ggttgtagta agtaatatag ctaacattat agctatacta
     5461 ctacgtttat tgcgtcgtac aaagtctaag gtgtattctt gaagaatatt catttcttca
     5521 ccaccttttc agagacaatc actccatctt ccatagttac aatacgattt gcctctagtg
     5581 ctattttctc atcatgagta ataagaagta ttgtctgatt aaatcgttta tttgataagt
     5641 ttaacaagtc tacaatttct tctgaatttt ttctatcaag attaccagtt ggctcatctg
     5701 ctaataaaat agctggtcta tagattaaac ttctagctat agataccctt tgttgttgtc
     5761 cacctgaaag ttgacttgga agatggttta atctatcaga taatcccaat atagatacaa
     5821 tctcagagaa tctatcctca tccacctttc ttttatcaag taacatgggt aataatatat
     5881 tttttctaac atcaagggta ggtataagat tataaaattg ataaataaga cctacttttc
     5941 tacgtctaaa aactgcaaga gcttcttcct ttaaacttga tatttctgta tcttcaacat
     6001 aaatctttcc agatgtaggt ctatctactc ccccaagcag atgtaacaat gtagatttgc
     6061 cagaaccaga tgctcctaca atggatacaa aatcaccttt ttgaactgat aaatttacat
     6121 tattaagggc tgtcactctg gtttgatttg aaccatatat ttttgtgaga ttttcacatt
     6181 ttaatatttc catatacaat catctccttt atggttaaat tttagcaacc taaaatgaca
     6241 ttatagtgac atgaatacaa atataaatta ctattaaaaa tattgtttag tttttgcaca
     6301 ttatttaaaa ttataatata aaacaaaaac tatgcaatat attttattta tactt
//
LOCUS       DAMJYR010000182         6303 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.187, whole genome shotgun sequence.
ACCESSION   DAMJYR010000182 DAMJYR010000000
VERSION     DAMJYR010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6303
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.187"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            247..1191
                     /locus_tag="PC471_003500"
     CDS             247..1191
                     /locus_tag="PC471_003500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HDF2790584.1"
                     /translation="MKFLRIEDEVIYSKKRYKLKREIDKLFLDEEYFQIEPQLFEEYD
                     EFTTINNKIPEESMVKVVNGKVMVLRADITTSIIKSLVPRWEDGLKLKLFYNSSIYKN
                     KNTVGIKEIRQIGCEYLGEASVEADREVVKLALKILEKYNNNFILEVGSSKYIHGLLE
                     ELNLNKNCENQIKNLLYTKNTHELKVYIEELKIKREVKELLSNILSLQGNLYNVIEKS
                     NKFYCNNKMKQALEELKQVNNLIEECNFLDKARFDLSMITMLDYYEGIMFRGYYPNSY
                     KEILSGGRYDSLTKEYGKEIPAIGFTLSVDELMKYVYK"
     gene            1213..1839
                     /locus_tag="PC471_003501"
     CDS             1213..1839
                     /locus_tag="PC471_003501"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HDF2790585.1"
                     /translation="MDYLTIALAKGRIEGESFKKFKKMGLGDSIDTDTRKLIFKDEEN
                     KIIYIHVKPSDVVTYVEKGVADLGIAGKDTILENETDVYEIYDLGFGKCKFAVAGLKG
                     DSIYREDEYLKVATKYPNIAKKYFKEKGQKIEIIKLNGSVELAPIVGLSDVIVDIVET
                     GNTLKANGLEILEDICNISARIISNRASYRFKYEQIQNIIRLFEELDN"
     gene            1873..2922
                     /gene="hisC"
                     /locus_tag="PC471_003502"
     CDS             1873..2922
                     /gene="hisC"
                     /locus_tag="PC471_003502"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HDF2790586.1"
                     /translation="MREKESIRELRGYEPNHVNCKVKLDANEGSKRLFKYLIKEISDS
                     DIDLNLYPEDSYSDLKESIIDYINISGVNKKNLLVGNGSSEIIDLIIHTFVDKDEVIL
                     SFSPSFSMYSIYSQINGSKFIGVESDENLVINIDNVIEKVKENNPKIVIVCNPNNPTG
                     TILKREEIIKLLDSTNSLVVLDEAYMDFGEESMLSDVFKYDNLIVLRTLSKAFGLAGI
                     RTGYMLSNSSLINSVEKVRPPYNLNSLSDFIATRALRNKDVVKAYIKEVKEEREVLYE
                     EMIGMGIKAYKSQANFILFYSEIENLSQKLIDRGVLIRKFGGKLENYYRVTIGDKEEN
                     SMFVGAIRDILKKEK"
     gene            2925..3503
                     /gene="hisB"
                     /locus_tag="PC471_003503"
     CDS             2925..3503
                     /gene="hisB"
                     /locus_tag="PC471_003503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HDF2790587.1"
                     /translation="MRIWKVERNTLETQILVELNIDGSGKAEIDTGIGFLDHMLTLMS
                     FHGKFDLKVICKGDTYVDDHHSVEDIGIAIGEAFKNALGDKKGIRRYSNIYIPMDESL
                     SMVAIDISNRPYLVFNAKFDTQMIGSMSTQCFKEFFRAFVNESRVTLHINLLYGENDH
                     HKIESIFKAFARALKEGSEIVSNEIASSKGVL"
     gene            3505..4110
                     /gene="hisH"
                     /locus_tag="PC471_003504"
     CDS             3505..4110
                     /gene="hisH"
                     /locus_tag="PC471_003504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HDF2790588.1"
                     /translation="MNIIVDYGLGNIDSVSRGFRKAGIETKISSDIDEIKQADSLILP
                     GVGAFRDSISALDKLGLIPIIKEHVSKGKFMIGICLGMQLLYEKSYEYGEYEGLGLIK
                     GSIDKLDISLKVPHMGWNNLKFNKANDDILKYINEDDYVYFVHSYYANSSNEELIAFS
                     EYEKKIPAIVRKGNVYGIQFHPEKSGEVGLNILRAYGEMIK"
     gene            4107..4829
                     /gene="hisA"
                     /locus_tag="PC471_003505"
     CDS             4107..4829
                     /gene="hisA"
                     /locus_tag="PC471_003505"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HDF2790589.1"
                     /translation="MIIFPAIDIKDNKCVRLTQGEFDKVNVYYDNPLEVAYKWKNEGA
                     EYIHIVDLNGARSEFGVNTKIIEDIANNIDIPIQVGGGVRDKEKVKSLINAGVTRVIL
                     GSIAIENLNLVEELVNEYKEKIVVSIDAKDGKVAVRGWEVVSNVDSLTLCKQLEKIGV
                     QTIVYTDISKDGMLQGPNFDIYERIAKETSLNVIASGGVTSIEDVKRLKAMNLYGAII
                     GKALYDKKIDFKEAQQLCLLGE"
     gene            4811..5569
                     /gene="hisF"
                     /locus_tag="PC471_003506"
     CDS             4811..5569
                     /gene="hisF"
                     /locus_tag="PC471_003506"
                     /EC_number="4.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000880088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HDF2790590.1"
                     /translation="MLTRRIIPCLDVRNGRVVKGKKFKDIVDVDSPEVLGKFYSDCGA
                     DELVFYDITASNEERKTSLEFVTKVAENINIPFCVGGGVNKLEDFTDILRKGADKVSI
                     NSSAVKNPELIREASLKFGAQCVVLSIDAKKNEEGSWSVYVKGGREKTNLDAIEWAVK
                     GVELGAGEIVVNSMDEDGMKNGYDIELLSKITSLVNVPVIASGGAGKKEDFYEAVNKS
                     NVDGILAASVFHFGEIKINDLKKYLKDMGVEVRL"
     gene            5597..6265
                     /locus_tag="PC471_003507"
     CDS             5597..6265
                     /locus_tag="PC471_003507"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HDF2790591.1"
                     /translation="MDNKCNNVYSDEVEKFIRSIKFDDKGLVPVVVQEVVSKDVLMLA
                     YMNKDVIKKTLKDKVACYFSRSRQELWVKGETSGNTQKVVKMSYDCDVDTILLFVEQT
                     GVACHTGNYSCFYRDLFDDSSKMELEVQTNILKELYDLINERKNNPVEGSYTNYLFEK
                     GIDKILKKVGEESSEVIIASKNTDKSELIYEISDLVYHTLVLMIEKGVEIDEIKKELL
                     KRRK"
BASE COUNT         2575 a          536 c         1250 g         1942 t
ORIGIN      
        1 ggtgagatta aatatagtaa aattaaatac aaaattaaat catcaccatc atcatagatt
       61 aaagggattg taatatgaaa ttaattcata gacacaagcc ctgtttttag tgtaattggt
      121 cttgtatcta tgatgggaag taattttaag agaaaattat agccatatag gtataaagcc
      181 tatgtggctt ttttattgtt ttaaatttgc aaatataaga gtaaatttaa tgaagagggt
      241 gagaatgtga aatttttgag gattgaagat gaggttattt attctaaaaa aagatataaa
      301 ttaaaaaggg aaatagataa actttttttg gatgaagaat attttcaaat agaaccacaa
      361 ttatttgaag aatatgatga atttacaact ataaataata aaattccaga agaatctatg
      421 gtaaaggttg taaatggaaa agtaatggta cttagagctg atataactac aagtataata
      481 aaaagccttg taccaagatg ggaagatggt ttaaagctta agctttttta taattcatca
      541 atatataaaa ataaaaatac agttggaata aaagagataa gacaaatagg ttgtgagtat
      601 ctaggagaag cctctgtaga agcagataga gaagtagtga aattagctct taagatactt
      661 gaaaaataca ataataattt tattctagag gttgggagta gcaagtatat tcatggtttg
      721 ttagaagaat taaatttaaa taagaattgt gaaaatcaaa ttaaaaattt actatatacg
      781 aagaacactc atgaactaaa agtatatatt gaagaattaa aaattaagcg tgaagtaaaa
      841 gagttattat caaatatttt aagtttacaa gggaatttat ataatgttat tgaaaaatca
      901 aacaagttct attgtaataa taagatgaag caagctcttg aagaattaaa gcaagtaaat
      961 aatttaattg aggaatgtaa ttttttagat aaggctagat ttgatttatc aatgattact
     1021 atgcttgact attatgaagg aattatgttt agaggatact acccaaactc atataaagaa
     1081 attcttagtg gcggaagata tgattcttta actaaagagt atggaaaaga aattccagcc
     1141 attggattta cactaagtgt tgatgagtta atgaagtatg tatataagta aagtttagaa
     1201 ggagggattg ctttggatta tttgactata gcactagcaa aaggacgaat tgaaggggaa
     1261 tcatttaaaa aatttaaaaa aatgggtcta ggagacagta tagacacaga tacgagaaaa
     1321 ctaattttta aagatgaaga aaataaaatt atatatatac atgtaaagcc atcagatgta
     1381 gttacatatg ttgaaaaagg agtcgctgac ctcggtatag caggtaaaga tactatttta
     1441 gaaaatgaga cagatgtata tgaaatatat gatttaggtt ttggaaaatg taaatttgct
     1501 gtagcaggat taaagggaga tagcatatat agagaagatg aatatttaaa agtggcaaca
     1561 aagtatccca atatagcaaa aaagtatttt aaagagaaag gtcaaaaaat tgaaataata
     1621 aaattaaatg gttctgtaga actagctcca atagtagggt tgtcagatgt aattgtagat
     1681 atagttgaga ctggcaatac acttaaagca aatggtcttg aaatattaga agacatatgt
     1741 aatataagtg ctagaattat atcaaataga gctagctata gatttaaata tgaacaaatt
     1801 cagaatataa ttcgtttatt tgaagaactt gataattaaa atataattga gctttataag
     1861 gagggattta agttgagaga aaaagaaagc ataagagagt taaggggata tgaaccaaat
     1921 cacgtaaact gtaaagtaaa acttgatgca aatgaaggaa gtaagaggtt gtttaaatac
     1981 cttattaaag aaatttcaga tagtgatata gatttaaatt tatatcctga agattcttat
     2041 agtgatttga aagaatctat aatagattac ataaatattt ctggagtaaa taagaaaaat
     2101 cttctagtag gaaacggttc aagtgaaata attgatttaa taatacatac ttttgtagat
     2161 aaagatgaag taatattgag tttctctcca agttttagta tgtattctat atatagtcag
     2221 ataaatgggt ctaaatttat aggtgttgaa agtgatgaaa atttagttat aaatatagat
     2281 aatgtgattg aaaaagtaaa ggagaataat cctaaaatag taattgtatg taatcctaat
     2341 aatccaactg gaacaatatt aaagagggaa gagataataa agcttttaga ttcaactaat
     2401 agtctagtgg ttttagatga agcatatatg gattttggag aagaaagcat gttaagtgat
     2461 gtttttaagt atgataatct aatagttctt agaactttat caaaggcatt tggattggct
     2521 ggaataagaa caggatatat gttatctaat agtagtttaa taaattctgt tgagaaagta
     2581 agacctccat ataatttaaa ttcattatca gactttattg ctacaagagc acttagaaat
     2641 aaagatgtgg taaaggctta tataaaggaa gtaaaagagg aaagagaagt tttatatgaa
     2701 gaaatgattg gtatgggaat taaagcatac aaatcacaag caaattttat attattttat
     2761 tctgaaatcg aaaatttatc acaaaaactt attgatagag gtgttttaat aagaaaattt
     2821 ggtggaaagt tagaaaatta ttacagagtt acaattggtg ataaagaaga aaattctatg
     2881 tttgttggag ctataagaga tattttgaaa aaggagaaat aggtatgaga atttggaagg
     2941 tagaaagaaa tactcttgaa acacagattt tagtagaatt aaatattgat ggttctggta
     3001 aagctgaaat agatactgga ataggatttt tagaccatat gcttacctta atgtcatttc
     3061 atggcaagtt cgatttaaaa gtgatttgta agggtgatac atatgtagat gaccaccata
     3121 gtgtagaaga tataggtata gctataggtg aagcatttaa aaatgcacta ggtgacaaaa
     3181 aaggaatacg aagatattct aatatctata tacctatgga tgaatctcta tcaatggtag
     3241 caatagatat aagcaataga ccttatcttg tatttaatgc taaatttgat acccaaatga
     3301 ttggtagtat gagtactcaa tgctttaaag aattttttag agcttttgta aatgaatcga
     3361 gagtaacatt acatatcaat ctcttatatg gggaaaatga ccatcataag atagaaagta
     3421 tttttaaagc atttgcaaga gctttaaaag agggtagtga aatagtttct aatgagatag
     3481 catcttcaaa gggggttttg tagaatgaat ataatagtag actatggtct tggaaatata
     3541 gattctgtgt ctaggggttt tagaaaagct ggaatagaaa ctaaaatttc tagtgatata
     3601 gatgaaataa aacaggcaga ttcacttata cttccaggtg ttggagcttt tagagattct
     3661 ataagtgcct tagataaact aggattgata ccaataataa aggagcatgt ttctaagggt
     3721 aaatttatga taggtatatg tctaggaatg caattacttt atgaaaaaag ttatgagtat
     3781 ggagaatatg aaggattagg acttataaaa ggaagtattg ataaactgga tataagttta
     3841 aaagtgccac atatgggatg gaataatcta aaatttaata aggcaaatga tgatatactt
     3901 aaatatataa atgaagatga ttatgtatac ttcgttcact catattatgc aaattcatca
     3961 aatgaagaac taattgcatt ttcagaatat gaaaagaaaa tccctgctat tgttagaaaa
     4021 ggcaatgtat atggtataca atttcatcct gaaaaaagtg gagaagtagg gttaaatata
     4081 ttaagagcat atggggagat gataaaatga taatatttcc agcaatagat ataaaagata
     4141 ataagtgtgt aagactcaca caaggagaat ttgacaaagt aaatgtatat tatgataatc
     4201 cattagaagt agcatataaa tggaaaaatg aaggagcaga atatatacat atagtagatt
     4261 taaatggtgc tagaagtgaa tttggtgtaa atacaaaaat aattgaggac atagcaaaca
     4321 atatagatat acctattcaa gttggcggtg gagttaggga taaggaaaaa gtcaaaagtt
     4381 taataaatgc tggtgttact agagtaatat taggtagtat agcgattgaa aatttgaatt
     4441 tagttgaaga attggtcaat gaatataaag agaaaattgt agtttctata gatgctaaag
     4501 atggaaaagt agcagttagg ggatgggaag ttgtaagcaa tgtagactca ttaacattat
     4561 gtaaacaact tgaaaagata ggtgtacaaa ctattgttta tacagatata tcaaaagatg
     4621 ggatgttgca aggtccaaat tttgacatat atgaaagaat agcaaaggaa acatcactaa
     4681 atgttattgc ttctggaggc gtaacttcta tagaagacgt aaaaagacta aaagctatga
     4741 acttatatgg tgcaataata ggtaaagcgc tttatgataa aaagatagac tttaaggagg
     4801 cacaacaatt atgcttacta ggagaataat accttgtcta gatgtgagaa atggaagagt
     4861 tgtaaagggg aaaaaattta aagatatagt agatgttgat agtccagaag tacttggaaa
     4921 attttatagt gattgtggtg cagatgaact tgtattctat gatataactg catcaaatga
     4981 agagagaaag acatctttag aatttgtgac aaaagttgca gaaaatataa atataccatt
     5041 ttgtgttggt ggaggagtaa ataaattaga agatttcact gatattttaa gaaaaggtgc
     5101 tgataaagtt tctataaatt catcagcagt caaaaatcct gaattaataa gggaagcttc
     5161 attaaaattt ggagcacaat gtgttgtttt atcgatagat gcaaagaaaa atgaagaggg
     5221 ttcatggagc gtatacgtaa aaggtggaag agaaaaaact aatctggatg caattgaatg
     5281 ggctgttaaa ggtgtagaac ttggagcagg agaaatcgtt gtaaatagta tggatgaaga
     5341 tggaatgaaa aatggatatg atatagaatt attatcaaag ataacttctt tagtaaatgt
     5401 acctgttata gcatctggag gcgctggaaa aaaggaagat ttttatgagg cggttaataa
     5461 atccaatgta gatggaattt tagcagcatc tgtttttcat tttggcgaga taaaaataaa
     5521 cgatttaaag aaatatttaa aagacatggg tgtagaagta agattataag ttaaatacta
     5581 aaaggagatt agaaatatgg ataataagtg taacaatgta tacagtgatg aagtagagaa
     5641 atttataaga agtataaagt ttgatgataa agggttagtc cctgttgttg ttcaagaggt
     5701 agttagcaaa gatgtactga tgttagcata tatgaataaa gatgtgataa agaaaacctt
     5761 aaaagataaa gtagcttgct attttagtag aagtagacaa gaactttggg ttaaaggtga
     5821 gacatctggt aatactcaaa aagtagttaa gatgtcttat gattgtgatg tagatactat
     5881 actacttttt gttgaacaaa ctggtgtagc ttgtcataca ggcaattatt cttgttttta
     5941 cagagacttg tttgatgata gttcaaaaat ggagcttgaa gtacaaacaa atatattgaa
     6001 agaattatat gatttaatca atgaaagaaa aaataatcca gttgaaggtt cttatactaa
     6061 ttatcttttt gaaaaaggaa tagataagat tcttaaaaaa gtaggtgaag agagtagtga
     6121 ggtaattata gcatcaaaaa atactgataa aagtgaactt atttacgaaa ttagcgattt
     6181 agtatatcat acattggttt taatgataga aaaaggtgtt gaaattgatg aaataaaaaa
     6241 agaattactt aaaagaagaa aatagaataa gatagattaa tgggtaataa gaaaatatac
     6301 cac
//
LOCUS       DAMJYR010000183         6245 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.188, whole genome shotgun sequence.
ACCESSION   DAMJYR010000183 DAMJYR010000000
VERSION     DAMJYR010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6245)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6245)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6245
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.188"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            164..1186
                     /locus_tag="PC471_003508"
     CDS             164..1186
                     /locus_tag="PC471_003508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDF2790592.1"
                     /translation="MKNLLEIQKKLIPQAIELMERRYSILRQISLSEPIGRRTLSNVL
                     EISERIIRSETEFLKEQGLIDVAVSGMTITKEGSDLLDKLKDIMSDIMGLSKLQDRVR
                     EKLGIKRVILVPGSCDENGSLLKDVTRYGCEYFLGILKDGDIVSITGGSTMLEFSNVI
                     KTDKRYPNSTIVPARGSMGTDVEMQSNNIVASISKKLHSNCKLLHIPDELDESAMKTL
                     SQVPEIKTTLEYIKRTDILVFSIGRADVMAKRRKLPEDKVDEILSKNAVGEAFGYYFN
                     KDGEIVYKLNTVGIDLETVKQVKETIAIFAGTEKVEALLAISNMHKNMVLVTDEESAY
                     KILSLL"
     gene            1246..2253
                     /gene="gap"
                     /locus_tag="PC471_003509"
     CDS             1246..2253
                     /gene="gap"
                     /locus_tag="PC471_003509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HDF2790593.1"
                     /translation="MVKVAINGFGRIGRLALRKMMEQQDKFEVVAINDLTDAKMLAHL
                     FKYDTAQGRFNGEIEVKEGAFVVNGKEIKVTAERNPADLPWAELGVDIVLECTGFFTS
                     KDKAEAHIQAGAKKVVISAPATGDLKTIVFNTNSDILDGSETVISGASCTTNCLAPMA
                     KVLNDKYGIEKGLMTTIHAYTNDQNTLDGPHPKGDLRRARAAAGNIVPNTTGAAKAIG
                     LVIPSLKGKLDGAAQRVPVVTGSITELVCTLGKNVTVEEINAAMKEASNESFGYTEEM
                     LVSSDIIGISYGSLFDATQTKVMEVDGKQLVKVVSWYDNEMSYTSQLIRTLGYFAQLA
                     K"
     gene            2475..3677
                     /locus_tag="PC471_003510"
     CDS             2475..3677
                     /locus_tag="PC471_003510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HDF2790594.1"
                     /translation="MSMLNKKTIEDIDVCGKKVLVRCDFNVPLQDGVITDENRLNGAL
                     PTIQYLISKGAKVILCSHLGKPKGEAKPELSLAPVAKRLSEMLGKEVVFAADDNVVGE
                     NAKKATEKMENGDVVLLENTRYRKEETKNEENFSKELASLAEIFVNDAFGTAHRAHCS
                     TVGAGEFLQERVCGYLIQKELKFLGEAVANPVRPFTAILGGAKVSDKLAVINELLEKV
                     DNLIIGGGMAYTFLKAQGYEVGTSLLEIDKVEYAKEMMEKAKNKGVNLLLPVDVVMAD
                     HFAPDATPIVTEDANVKEDYMGLDMGPKTIANFVKTIKESKTVVWNGPMGVFEFENFA
                     NGTLSVARAMAELTDATTVIGGGDSAAAVNQLGFGDKMTHVSTGGGASLEFLEGKELP
                     GIAALDNK"
     gene            3703..4446
                     /locus_tag="PC471_003511"
     CDS             3703..4446
                     /locus_tag="PC471_003511"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HDF2790595.1"
                     /translation="MRKPIIAGNWKMHKTIKEALEFVNEVKDKVNSDKVEAVICAPFT
                     LLKDLKEATKGTNIKIGAQNMHFEEKGAFTGEVSPLMLKEIDMDYVVIGHSERRQYFN
                     ETDETVNKKVLKALEVGIDPILCVGETLEQREAGKTKDVCKVQVEKALENVLKDDLAK
                     VVVAYEPIWAIGTGKTATAEDANDVISYIREVIKGLYGELANEVRIQYGGSVKPSNVA
                     EIMGQSDIDGALVGGASLASNDYLDLVNF"
     gene            4455..5987
                     /locus_tag="PC471_003512"
     CDS             4455..5987
                     /locus_tag="PC471_003512"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003228330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HDF2790596.1"
                     /translation="MMKKPVALIIMDGFGYNKDVKGNAIAESKTPNLDRIKKEYPNTL
                     INASGLDVGLPDGQMGNSEVGHTNIGAGRIVYQDLTRITKSIKDGDFFTNKVLCEAMD
                     NAKENSLHVMGLLSDGGVHSHIDHLKAIIKMAKDKGVQKVYVHAFTDGRDTDPQSALE
                     YAKEVQASMDEIGVGEFATVSGRYYAMDRDKRWERVELAYNAMVRGIGEKANSIEEAI
                     QNSYDDGKNDEFIMPTVIMKDDKPVGSIKENDSIIFFNFRPDRARQITRALVCEEFDG
                     FKREDIKNFFVCLTEYDITIENVHIAFGPQSLANTLGEYLAKNGKTQLRAAETEKYAH
                     VTFFFNGGVEEPNKGEERLLIPSPKVATYDLKPEMSAYELTDKALDKLGEDKFDFIVL
                     NFANPDMVGHTGSIEAAIKAVETVDTCVGRLIDKIVELGGSAIITADHGNAEYMLDPE
                     TGKTVTAHSINPVPFIVVGQEYESAKLLDGGRLSDIAPTILDMMKLEKPEEMTGHSLI
                     SK"
BASE COUNT         2499 a          731 c         1250 g         1765 t
ORIGIN      
        1 tgaaaatggt ggagaaatat tttataaaat aaaaaaataa aaaagttttt gaaaaactat
       61 tgaaatctat acgcaaatcc tataaaataa aaatatcttg tgggacttaa ataaacacta
      121 gggacaaaaa aagtcccgaa aggaaagcaa taaggagtat gttatgaaga acttattaga
      181 aattcaaaag aaattgattc cacaggcaat tgaacttatg gaaagaagat actctatttt
      241 aagacaaata tctttaagtg aaccaatagg taggagaaca ttatcaaatg tattagagat
      301 tagtgaaaga ataatcaggt cagaaactga gtttttaaaa gaacaaggac ttatagacgt
      361 agctgtatct gggatgacta tcactaaaga aggttctgat ttgttagata aacttaagga
      421 catcatgagt gacatcatgg gattgtctaa gttacaagat agagtcagag aaaagcttgg
      481 aataaaaagg gtaatattag ttcctggaag ctgtgatgaa aatggaagtt tactaaaaga
      541 tgtaactaga tatggatgcg aatatttctt aggcatttta aaagatggag atatcgtttc
      601 aataacagga ggaagcacta tgttggagtt ttcaaacgtt attaaaactg ataagagata
      661 tccaaactct acaatagttc cagctagagg aagtatggga actgatgtag agatgcaatc
      721 taacaatata gttgcttcaa taagtaagaa attacattct aattgcaaac ttttacatat
      781 accagatgag ttggatgaaa gtgcaatgaa aactcttagc caagtgccag agattaagac
      841 aactttggag tatattaaaa gaacagacat tttagttttt agtataggta gagcagatgt
      901 catggcaaag agaagaaaac ttccagaaga caaggttgat gagatattat ctaaaaatgc
      961 agttggagaa gcatttggat attatttcaa caaagatgga gaaatagttt acaaactgaa
     1021 cactgttgga attgatttag aaacagttaa acaagtaaaa gaaactattg caatctttgc
     1081 agggactgaa aaagtagaag ccttattagc tatatcaaat atgcataaaa acatggttct
     1141 tgtaacagat gaagaaagtg cttataagat attgtcatta ttataaaagt caagacctag
     1201 cacagagcta gaaaacatac atttacttta taggaggtag cgaaaatggt taaagtagct
     1261 ataaatggat ttggaagaat aggaagatta gcattaagaa aaatgatgga gcaacaagat
     1321 aagttcgaag ttgttgcaat aaacgactta acagacgcta aaatgttagc tcatttattt
     1381 aaatatgata cagctcaagg aagatttaac ggagaaatag aagttaaaga aggtgctttt
     1441 gtagtaaacg gaaaagaaat aaaagtaaca gcagaaagaa atcctgctga cctaccatgg
     1501 gctgaattag gagtagatat agtattagaa tgtacaggat tctttacatc taaagataaa
     1561 gctgaagctc atatacaagc aggggctaag aaagttgtta tatctgcacc agctactgga
     1621 gatttaaaaa caatagtatt caacacaaac agtgacatat tagatggttc tgaaacagtt
     1681 atatcaggag cttcttgtac tactaactgt ttagctccaa tggctaaagt attaaatgat
     1741 aaatacggta tagaaaaagg cttaatgact actatacatg catacactaa tgaccaaaat
     1801 actttagatg gtcctcatcc aaaaggtgat ttaagaagag ctagagctgc tgctggaaac
     1861 atagttccaa acactacagg agctgcaaaa gcaataggct tagtaatacc ttcattaaaa
     1921 ggaaaattag atggggcagc acaaagagta ccagttgtaa ctggttcaat aactgaatta
     1981 gtttgtacat taggaaaaaa tgttacagtt gaagaaataa atgcagctat gaaagaagct
     2041 tcaaacgaat ctttcggata tactgaagaa atgttagtat cttctgacat aatcggaata
     2101 agctacggtt cattatttga cgctactcaa acaaaagtta tggaagtaga tggaaaacaa
     2161 ttagttaaag ttgtttcttg gtatgataat gaaatgtctt atacttctca attaataaga
     2221 actttaggat actttgcaca attagcaaaa tagtcagttt tgagaaaata gaaaagtaga
     2281 ttgacaaagt ccgagtttgt agtttaatac tacggactca ggactttttt taagaaaaaa
     2341 tatataaaat acacctaaat aatgttacat actatatata aaaatatgat aatatgatat
     2401 atttaatagg gtaaaagtat gtactatatt atacttggca ataaaaaatt actttattaa
     2461 aaggagagat atttatgtca atgcttaaca aaaaaactat agaagatatt gatgtgtgcg
     2521 gaaaaaaagt attagtaaga tgtgacttta atgtgccatt acaagatggt gtaattactg
     2581 atgaaaatag attgaacgga gctttaccaa ctattcaata tttaatttct aaaggggcaa
     2641 aagttatatt atgctctcac ttaggaaagc caaaaggaga agctaaacca gaattatctt
     2701 tagcaccagt agcaaagaga ttatctgaga tgttaggaaa agaagttgta tttgcagcag
     2761 acgataatgt tgtaggagaa aatgcaaaga aagcaactga aaaaatggaa aacggagatg
     2821 tagtattatt agaaaatact agatatagaa aagaagaaac taaaaacgaa gagaatttct
     2881 ctaaagaact agcatcactt gctgaaatat ttgtaaatga tgcatttgga actgctcata
     2941 gagcacattg ctcaacagtt ggtgcaggcg agtttttaca agagagagtt tgtggatact
     3001 taattcaaaa agaattaaaa ttcttaggag aagctgttgc aaaccctgta agaccattta
     3061 cagctatact tggaggagct aaagtttctg ataaattagc tgttataaac gaattattag
     3121 aaaaagttga taatctaata ataggtggag gaatggctta tacattctta aaagctcagg
     3181 gttatgaagt aggaacttct ttacttgaaa tagataaagt tgaatatgct aaagaaatga
     3241 tggaaaaagc taagaataag ggtgttaact tattattacc agtagacgtt gttatggcag
     3301 accactttgc accagatgct actccaatag taacagaaga tgctaatgta aaagaagatt
     3361 acatgggact agatatggga cctaagacta tagcaaactt tgtaaaaact ataaaagaat
     3421 caaaaactgt agtatggaat gggccaatgg gagtatttga atttgaaaac tttgcaaatg
     3481 gtacattatc agtagctaga gctatggcag agttgactga tgcaactact gtaattggcg
     3541 gtggagatag tgcagcagca gttaatcaat taggatttgg tgacaagatg actcacgtat
     3601 caacaggtgg aggagcatca ctagaatttt tagaaggtaa agaattacca ggtattgcag
     3661 cattagataa taaataaact aatatataga ggagattgta aaatgagaaa acctataatt
     3721 gcaggaaact ggaaaatgca taaaacaata aaagaagctt tagaatttgt aaatgaagtt
     3781 aaagataaag taaattctga taaagtagaa gctgttatat gtgcaccatt tactttatta
     3841 aaggatttaa aagaagctac taagggtaca aatataaaga taggtgctca aaatatgcac
     3901 tttgaagaaa aaggagcatt tacaggagaa gtttcacctc ttatgttaaa agaaatagat
     3961 atggactatg ttgtaatagg acattcagaa agaagacaat acttcaatga aactgatgaa
     4021 actgtaaaca agaaagtttt aaaagcatta gaagtaggaa tagacccaat attatgtgtt
     4081 ggagaaactt tagaacaaag agaagctgga aaaactaaag atgtttgtaa agttcaagtt
     4141 gaaaaagctt tagaaaatgt tcttaaagat gatttagcaa aagtagttgt tgcatatgag
     4201 ccaatctggg ctattggaac tggtaaaact gcaactgctg aagatgctaa tgacgttata
     4261 tcttatataa gagaagttat aaaaggatta tatggagaat tagctaatga agttagaata
     4321 caatacggtg gaagtgttaa accttcaaat gtagccgaaa taatgggtca aagtgatata
     4381 gatggagctt tagtaggtgg agctagttta gctagtaatg attacctaga ccttgttaat
     4441 ttctaaggag tgaaatgatg aaaaaaccag ttgctttaat tattatggac gggtttggat
     4501 acaataagga tgttaaggga aatgcaattg ctgagtccaa aactcctaat ttagatagaa
     4561 taaaaaaaga gtatccaaac acattgataa atgctagtgg tcttgatgtt ggtctacctg
     4621 atggtcaaat gggaaattca gaagttggac acactaacat aggtgcaggt agaatagtat
     4681 atcaagattt aactagaata acaaaatcta taaaagatgg tgactttttt actaataaag
     4741 ttttatgtga agcaatggat aatgcaaaag aaaactctct tcatgtaatg ggacttttgt
     4801 ctgatggagg agtacattca catatagacc atcttaaggc tataatcaaa atggcaaaag
     4861 ataaaggcgt acaaaaggtg tatgttcacg catttactga tggtagagat actgaccctc
     4921 aaagtgcatt agaatatgcc aaagaagtac aagctagtat ggatgaaatt ggagtaggtg
     4981 aatttgctac agtttctggt agatactacg ctatggatag agataagaga tgggaaagag
     5041 tagaacttgc atacaatgcc atggttagag gtattggtga aaaagcaaat tctattgagg
     5101 aagctattca aaactcttat gatgatggaa aaaatgatga gtttataatg ccaacagtta
     5161 ttatgaaaga tgataaacca gttggaagta taaaggaaaa tgattcaatt atattcttca
     5221 actttagacc agatagagct agacaaataa ctagagcttt agtttgtgaa gagtttgatg
     5281 gatttaaaag agaagatatt aaaaacttct ttgtatgctt aacagagtac gatataacta
     5341 tagagaatgt gcatatagca tttggacctc aatctttagc caacactcta ggtgagtatc
     5401 tagctaaaaa tggaaaaact caacttagag ctgctgaaac tgaaaagtat gcacatgtaa
     5461 ctttcttctt caatggtgga gtagaagagc caaataaagg tgaagaaaga ctattgatac
     5521 cttctccaaa ggttgcaact tatgatttaa aaccagaaat gtcagcatat gaattgacag
     5581 ataaagcttt agataaatta ggagaagata agtttgactt tatagtatta aactttgcaa
     5641 atcctgatat ggtaggtcac acaggaagta tagaagcggc tataaaggct gttgaaactg
     5701 tagatacttg tgtaggaaga ttgatagata aaatagtaga gttaggtgga agtgcaataa
     5761 taactgctga ccacggaaat gctgaatata tgttagaccc agaaacagga aaaactgtaa
     5821 cggctcactc tattaatcca gtaccattta ttgtagttgg tcaagaatat gaaagtgcaa
     5881 aattattgga tggaggaaga ttatctgata tagcacctac tattttagat atgatgaagt
     5941 tagaaaagcc agaagaaatg actggtcatt cacttatttc aaaatagtcg attctgataa
     6001 gtagataata atatataaac tacaatataa atttaaaaat aatgcagttt aggtttattt
     6061 tgtaaagttt agactttatg acataaggtt taaattttgt aagttctgga tattgtaagg
     6121 tgtagatgaa gatatatttt ataaaattta caagatagac aatttataaa ataaaaaatt
     6181 acaatataga taataatgta aataatgata taaataataa caagatagac aataacaata
     6241 taaat
//
LOCUS       DAMJYR010000184         6202 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.189, whole genome shotgun sequence.
ACCESSION   DAMJYR010000184 DAMJYR010000000
VERSION     DAMJYR010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6202)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6202)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6202
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.189"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            287..1654
                     /locus_tag="PC471_003513"
     CDS             287..1654
                     /locus_tag="PC471_003513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase"
                     /protein_id="HDF2790597.1"
                     /translation="MGLKEFDFIEESIDMLRTMSPTLETISDEIEEYFENILDEKNQE
                     YINVTSRIKSESSLREKIIRNRYLKKYGEASNLIHNVSDLIGLRIECRFIEDENKIYR
                     LLRRYFNKTDDKINYYNKENKNIKLKLSERQPNKQKNGFEIYKIDGVFAYLDREVKFE
                     LQIKSLVNVFWSEIEHKIIYKNNTYLLADKFIKDMMDSIKNNLTMIDNQLLSIYKNFH
                     SGKSFNMKVSKKEIEKLFAKLVYDAFSEKMNKSIGFVVDFKKPCETILNYSFNKQDIS
                     DEVLGNFMLDEFARLNEIVNKDIDFNEQIEFEREPTFDDKFCKDLGNHFRSRLNTEFP
                     WNLFFRILFEIEPYNNTKDFENFVEFIKENVLLEENREQLLCQFEEDSKLIIEDIYDC
                     IFNSISEIDSVEILYNYNLEKINFTSSEIINCICREYECYGEYLEEKEKLMNTFKEKI
                     IQIFE"
     gene            1679..2287
                     /locus_tag="PC471_003514"
     CDS             1679..2287
                     /locus_tag="PC471_003514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HDF2790598.1"
                     /translation="MRHFSNIYELSKIILEEAICIEKTGVILAGGKNSRMGRDKAFLE
                     LHDKLFIEIAIEAFKNFDELIIISNNEELYSKYDIKVYNDIVKDVGPIGGIYTALEYA
                     KYDIVTIACDMPYLNNQIVERIANKMDDKSVISVTNGKLQPLCSGYKKSIINKVSLCI
                     RENDLKLRSFIDKIDKSYIYFDEEDLFLNVNTVDEYKKLTKV"
     gene            2335..2706
                     /locus_tag="PC471_003515"
     CDS             2335..2706
                     /locus_tag="PC471_003515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HDF2790599.1"
                     /translation="MNLLKCDSWAVILNNSDKESKAYKILDELKRNMYKVVAIDEEKK
                     PIEGIDVYECLKDVPHNIDVVAIIDKQSKMDVILEEVELLDIQNMWFEKGSFSEMMIK
                     KTKDLKLNIEYNLSLYDELTR"
     gene            2885..3223
                     /locus_tag="PC471_003516"
     CDS             2885..3223
                     /locus_tag="PC471_003516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HDF2790600.1"
                     /translation="MLIVTTEKVEGKKISKVLGLVRGSTIRAKHVGKDIGASFKNLVG
                     GELTGYNEMLTEARQIAIGRMVEDAEAKGANAVIAFRLSSASVMQGAAEMLAYGTAVV
                     LEDDNSILEK"
     gene            3689..4192
                     /locus_tag="PC471_003517"
     CDS             3689..4192
                     /locus_tag="PC471_003517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MogA/MoaB family molybdenum cofactor
                     biosynthesis protein"
                     /protein_id="HDF2790601.1"
                     /translation="MFNVAIITLSDKGYEGKREDITGKKLTEFVENTGVYKVTEYVLI
                     KDDKEMLKENIIRLCNSNKIDLILTNGGTGFSKRDITPEATKEVLEKEIPGLSEYMRM
                     KSTEITKKAILSRGVSGIRNNSIIINLPGSPKGAVENLSFIIDVLDHGIEVLRGEATE
                     CATKKSE"
     gene            4239..5198
                     /gene="moaA"
                     /locus_tag="PC471_003518"
     CDS             4239..5198
                     /gene="moaA"
                     /locus_tag="PC471_003518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HDF2790602.1"
                     /translation="MIDKYGRKIDYLRISLTDKCNLRCVYCMPPDVKFDKNYINENLS
                     FEDYKFIIKAMAEQGITKIRFTGGEPLLYPKLNELIKFTKEECGINNIGMTTNAIGLS
                     DRIFELEKCGLNKVNISLDSLKEYKYKSITRGGNLKDVLKSIESCLNLGIKVKLNCVA
                     ISGFNDDELKDFMFLTVKSPIDVRFIELMPLGEANRVYKKGYFNVKEMIEKIYGLYKV
                     KNDEKSTAEYYMFKGAKGRIGIITPLSCSFCNTCNRIRLTSSGAIKLCLHSKEEIDIK
                     PFLHKPLLFRESMKDIIKQKPEKHHLIENKCSDTNRRMYEIGG"
     gene            5202..5672
                     /gene="moaC"
                     /locus_tag="PC471_003519"
     CDS             5202..5672
                     /gene="moaC"
                     /locus_tag="PC471_003519"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HDF2790603.1"
                     /translation="MLTHINEKGYAKMVDISEKDDTKRTAVATATITLSKEALEKVKN
                     GQIKKGDVLSVAQVAGIMGAKNTSSNIPMCHQIKLVGCDLEFDVDDSMSAIKIYATCK
                     SLGKTGVEMEALNACSLAALTIYDMCKAVDKKMVISNIHLVEKTGGKSGDFKFE"
     gene            5708..6139
                     /locus_tag="PC471_003520"
     CDS             5708..6139
                     /locus_tag="PC471_003520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HDF2790604.1"
                     /translation="MAKVVSINISEKKGVIKVPVQCAELKINHGIVGDAHAGNWHRQI
                     SLLGKESIDKMKMKGFDQLKYGDFAENVTTEGIEVFSLPVGTKLRIGQCEVEVTQIGK
                     KCHNGCEIKKLTGDCVMPREGIFVKVLKEGIINVNDSIDVI"
BASE COUNT         2622 a          502 c         1121 g         1957 t
ORIGIN      
        1 aatttttata tagtaaatga caaaagttta caataaatgt atattttctt actcattcct
       61 taaaattact catttagttt aataactctc atttcatatg taaagttggt aaaaaaatta
      121 aaattaaaag taaaaaacta aataaattaa tgttaatttg aaatatatat gaaggttttt
      181 ggagaatact tacttatgat aggaaaaaat gttaaaataa tagtgtaatc aaatataaag
      241 gactgaaata attaacagtt attaaatatt aggagtgtaa aaatttatgg gattaaaaga
      301 atttgatttt attgaagagt ctattgatat gttgaggaca atgtcaccaa cattagaaac
      361 tatatcagat gaaatagaag aatattttga aaatatcttg gatgaaaaga atcaagaata
      421 tataaatgta acctctagaa taaaatcaga atcaagttta agagaaaaga taattagaaa
      481 tagatattta aaaaaatatg gagaagcaag taatcttatt cataatgttt ctgacttaat
      541 aggcctaaga attgagtgta gatttattga ggatgaaaac aaaatatata gacttttgag
      601 aagatatttt aataaaacag atgacaaaat aaactattat aataaagaga ataagaatat
      661 taaattaaaa ctatctgaga ggcaacccaa taagcaaaaa aatggatttg aaatatataa
      721 aatagatgga gtttttgcgt atttagatag agaagtaaaa tttgaacttc aaattaaatc
      781 tcttgtaaat gtgttttgga gtgaaataga acataaaata atttataaaa acaatacata
      841 tttacttgct gataaattta taaaggatat gatggactct ataaagaata atcttactat
      901 gatagataat cagcttttaa gtatttataa aaactttcat tcagggaagt cttttaatat
      961 gaaagtaagt aaaaaagaaa ttgaaaaatt atttgcgaaa ctagtctatg acgctttttc
     1021 tgaaaagatg aataaaagta taggttttgt tgtagatttt aaaaaaccat gtgagactat
     1081 attgaactat tcttttaata aacaagatat aagtgatgaa gttttaggta attttatgct
     1141 tgatgagttt gcaagattaa atgaaattgt aaacaaggat atagatttta atgagcaaat
     1201 agaatttgaa agagaaccaa catttgatga taagttttgt aaagacctag gaaatcattt
     1261 tagaagtaga ttaaacacag aatttccatg gaacctattt tttagaatat tatttgaaat
     1321 agagccatat aacaatacca aagactttga aaactttgta gagtttataa aagaaaatgt
     1381 gctactagaa gaaaataggg agcaattatt atgccaattt gaagaggata gtaagcttat
     1441 aattgaagat atatatgatt gtatatttaa tagtataagt gagattgata gtgttgaaat
     1501 tttatataac tataatcttg aaaaaataaa ctttacatca agtgaaatca taaattgtat
     1561 atgtagagaa tatgagtgtt atggtgaata cttagaagaa aaagaaaagc ttatgaatac
     1621 atttaaagaa aagattattc aaatctttga gtaataggtc aatgctgaag tggcgctaat
     1681 gcgccatttt tcaaatatat atgaattaag taaaattatt ttggaggagg cgatttgtat
     1741 agagaaaaca ggtgtaattt tggcaggggg aaaaaattct agaatgggta gagataaagc
     1801 ctttttagag ttacatgata agctttttat tgaaattgca atagaagcat ttaaaaactt
     1861 tgatgagttg ataataatat ctaacaatga agaattatat tctaaatacg atattaaagt
     1921 ttataatgat atagtaaaag atgttggtcc aattggagga atatatactg ctcttgaata
     1981 tgcaaagtat gatatagtta caattgcatg tgatatgcca tatctaaata atcaaatagt
     2041 cgaaagaata gctaataaaa tggatgataa aagtgtgatt tcagtgacaa atggaaagtt
     2101 acaaccattg tgttctggat ataaaaagag tataataaat aaagtatctt tatgtataag
     2161 ggaaaatgat ttaaagctca gaagctttat agataaaata gataaaagtt atatatactt
     2221 tgatgaagaa gatttatttt taaatgtaaa cactgttgat gagtataaaa aattaactaa
     2281 agtttaatta gctatggttg taaaatagag taggatttat atggaggaga gtttatgaat
     2341 ttacttaaat gcgattcttg ggcagtaata ttaaataata gcgataaaga aagtaaagca
     2401 tataaaatat tagatgaact taaaaggaat atgtacaaag ttgttgctat agatgaagag
     2461 aaaaaaccta ttgaaggaat agatgtgtat gaatgtttaa aagatgttcc acacaatatt
     2521 gatgttgtag ctattattga taaacaatct aagatggatg tgattttaga agaagtagag
     2581 cttttggata tacaaaatat gtggtttgaa aaaggaagct ttagtgaaat gatgattaaa
     2641 aagactaaag atttaaaatt aaatatagaa tataatttaa gtttatatga tgagttgact
     2701 agataaaatt atatttctta ataataaaaa tataattagt taataaaaca tattatttta
     2761 tagtatagat tggttaataa aagtacagat tggttaatga aacattaaat tacaatattt
     2821 gttaaaaaaa ttgtgagtta catgatataa tgtattaaat aggatactaa ggagttgata
     2881 tattatgtta atagtaacta cagaaaaagt agaaggtaaa aagatatcaa aggttttagg
     2941 attagtgaga ggaagtacaa taagagcaaa acatgttgga aaagatatag gagcaagttt
     3001 taagaatctt gttggaggag aacttactgg atataatgaa atgctcactg aagcaagaca
     3061 aattgccata ggtagaatgg ttgaagatgc agaagctaaa ggtgcaaacg cagtaatagc
     3121 atttagactg tcttcagctt cagttatgca aggagcagca gaaatgcttg cttatggaac
     3181 agcagttgtt ttagaagatg ataatagtat tcttgaaaaa taaaatataa atagttaaaa
     3241 agaggcatct tttaaaaatt ttatggatgc ctttttttat ttgaaaaaac taatttgaga
     3301 agaaatttta actattatga caataaaatt agggtgatat tatataaata agtaaaatta
     3361 tattatataa tgtatggtat agactttaaa aattttattt gtagagaaaa atatggacaa
     3421 tgttataata attatagaaa ttaaaagtac ttacattcat tatgtatgtg acatgtataa
     3481 ttagttttat atttaataca gttttgaagt aatatcttca ttttgagaac gttggaataa
     3541 taaaaaatgt attattatag gtgatataaa taaaaaataa atataatatg ttatattgct
     3601 gatagaaaag agaataaaag aaaataggtt ttattaaaag agatttttat ttataggact
     3661 taatcaatgt atagaggggt gagaacatat gtttaatgtt gcaattataa cacttagtga
     3721 taaaggatat gaaggaaaaa gagaagatat aacaggaaaa aagttaactg aatttgttga
     3781 aaatacagga gtttataaag ttactgaata tgtacttata aaagatgata aggaaatgct
     3841 aaaggaaaat attataagac tttgtaatag taataaaata gatttaattc ttacaaatgg
     3901 aggtactggt ttttcaaaaa gagatataac cccagaagca actaaggaag tgttggaaaa
     3961 agaaatacca ggtctgagcg aatatatgag aatgaaatcc acagaaatta ctaaaaaggc
     4021 catactaagt aggggtgtga gtggaataag aaataattct ataataataa atttacctgg
     4081 aagtcctaaa ggtgcagttg aaaatctaag ttttataatt gatgttttag accatggcat
     4141 agaagtttta agaggagaag ctacagaatg tgccactaaa aaaagtgaat agtatataat
     4201 ctagatagat aaaaaataga caaagaggta gataaagtat gattgataag tatggaagaa
     4261 aaatagacta ccttcgtatt tcacttacag ataagtgtaa tttaaggtgt gtctattgta
     4321 tgccaccaga tgtaaaattt gataaaaatt atataaatga aaatctaagc tttgaagact
     4381 acaagtttat tataaaggct atggctgaac aaggtataac aaaaattaga tttactggag
     4441 gagaacctct tttatatcct aaattaaatg agcttataaa atttacaaaa gaagaatgtg
     4501 gaataaataa cataggaatg accactaatg ctattggtct ttctgataga atttttgagc
     4561 ttgaaaaatg tggcttaaat aaagttaata taagtttaga ttctttgaag gaatataagt
     4621 ataaatccat aacaagagga ggaaacttaa aagatgtctt aaaatcgatt gaatcttgtt
     4681 taaatttagg tataaaagta aagttaaatt gcgttgctat aagtgggttt aatgatgatg
     4741 agcttaaaga ttttatgttt ttaacagtta aatcaccaat tgatgtaaga tttatagagc
     4801 ttatgccatt aggtgaggct aatagagttt ataagaaggg ttattttaat gtaaaagaaa
     4861 tgattgaaaa aatatatggt ttatataaag tcaaaaatga tgaaaaaagt acagcagaat
     4921 attatatgtt taaaggagct aaaggaagaa taggaattat aactccatta agctgttcat
     4981 tttgtaacac ttgtaatcgt attagactta cttctagtgg tgcaataaaa ttatgtctac
     5041 attcaaaaga agaaatagat ataaaaccat ttttacataa accattgtta tttagagaat
     5101 ctatgaaaga tataataaaa caaaaaccag aaaaacatca tttaatagaa aataaatgta
     5161 gtgatacaaa taggcgtatg tatgaaattg gaggataagt aatgttaaca catataaatg
     5221 aaaaagggta tgccaagatg gtggacataa gtgaaaaaga tgatactaaa agaacggccg
     5281 tagctacagc tacaattact cttagtaagg aagcattaga aaaagtaaaa aatggtcaga
     5341 taaaaaaagg agacgtattg tctgtagctc aagttgcagg tataatggga gctaaaaata
     5401 catcttcaaa cataccaatg tgccatcaga ttaaattagt tggatgtgat ttagaatttg
     5461 atgtagatga ttctatgtct gctattaaga tatacgcaac ctgtaaatct cttgggaaaa
     5521 ctggagtaga aatggaagct ttaaatgcat gttctttagc agcacttaca atttatgata
     5581 tgtgtaaagc agttgataaa aaaatggtta taagtaatat ccatcttgtg gagaagactg
     5641 gtggaaaatc aggagatttt aagttcgagt aaattaataa tatattgggt taaaatttgg
     5701 aggaaatatg gcgaaggtag tgtcaataaa tataagtgag aaaaagggtg taattaaagt
     5761 tcctgtacaa tgtgcagagc taaaaataaa tcatgggata gttggagatg cacatgcagg
     5821 aaactggcat cgtcagataa gcttacttgg aaaagaaagc atagataaaa tgaaaatgaa
     5881 aggctttgac caattaaaat atggagattt tgctgaaaat gtaactacag aagggataga
     5941 agttttttcc ctgcctgttg gtacaaagtt aagaataggg caatgtgaag ttgaagtaac
     6001 tcaaataggg aaaaaatgtc acaatggatg tgaaattaag aaattgacag gtgactgtgt
     6061 gatgccacga gaaggaattt ttgtaaaggt tctaaaagaa ggaattataa atgtgaatga
     6121 ttcaatagat gtaatctaat gaagtcacta ttaaaatata attataagta aatactatta
     6181 agatttaaat atatatttta ag
//
LOCUS       DAMJYR010000185         6192 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.190, whole genome shotgun sequence.
ACCESSION   DAMJYR010000185 DAMJYR010000000
VERSION     DAMJYR010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6192)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6192
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.190"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            226..1551
                     /gene="cdeA"
                     /locus_tag="PC471_003521"
     CDS             226..1551
                     /gene="cdeA"
                     /locus_tag="PC471_003521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter CdeA"
                     /protein_id="HDF2790605.1"
                     /translation="MENLFTRKFTTFEFLKFVSPAIISMIFISLYTIIDGIFVSTLVG
                     SDALASINIVLPIINLVCGFGIMMATGGGAIVSIRMGENRQDEANSTFSFIVLFSLIV
                     GILFTVISYFFIKEISILLGATDKLLPYCITYGKVMILCTPFYILKFIFEYFARTDGN
                     SKFSLFLSVIGGVTNIILDYVFIKYFGMGLLGAAVATAIGIILTCVLGIIYFLSNKST
                     LKLRKPKTDFRLIRDTMINGSSEMVTELSTGITTFLFNVVALKLAGENGLAALTIVLY
                     AHFLMTSVYLGFAAGVSPLISYNFGAENSDKLKETFKHSLKFIFVSSLLVFIFALVFA
                     PFIVRVFVSPDNTVFKLALQGLKIFAFAFLFVGINIFASGFFTAFHNGKISAIISFSR
                     AFVFIIIGIIILPPMLNMTGLWLTVPFAEVITIFISILFIKKYKCRYKY"
     gene            complement(1646..2440)
                     /locus_tag="PC471_003522"
     CDS             complement(1646..2440)
                     /locus_tag="PC471_003522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2790606.1"
                     /translation="MNELKPYIKFFNKNTKLYIIIFMIVGIVMNILPVFILKIFGEAS
                     KTNLSTIVNPILTYITILLFVYGVNIANKDFSGALSIRADRKSCIKAIVIDLIILSFV
                     ISILGIVLIFLSKLFIEIITGYSIELSVLLKRDMIWTSISALIDPSTSSMPLTYLNYF
                     VQICINEICIGFIGVLLGAFTYRVRKVTSITILIGLPILMIIYIVNFATKNMHKMLLY
                     LEKIIYSFQNPFILFGTKVGIIVICIIFIILLLRKAPIKEYANDLL"
     gene            complement(2441..3316)
                     /locus_tag="PC471_003523"
     CDS             complement(2441..3316)
                     /locus_tag="PC471_003523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2790607.1"
                     /translation="MSSSIKIENLSHSFKKQNIFNNMSLSFEKNKIYGLLGKNGAGKT
                     TLLNIMVNQLIQNEGEIEILGKNPKEDIKVLEEVCIVREKEFYSPDFKVGQIFEFSSS
                     FYKSYDKGLEEELCKYFDLNTKKKYKQLSRGMKTILSNIIGICSNSAITIFDEPTIGL
                     DAVNRQEFYNIILDSYIKNPRTIIISTHLIDEIDDLLEHVIILNEGKIIIDEEIDVIK
                     QKAHYITGNKEELEKLECIKNIKPKKAFGNAVAYFYYGDFSESDEKILKNSNIDVGYI
                     GLQDMFVNMTKKEEL"
     gene            complement(3320..3688)
                     /locus_tag="PC471_003524"
     CDS             complement(3320..3688)
                     /locus_tag="PC471_003524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDF2790608.1"
                     /translation="MKLVLNNEEPIFIQIARAIEDEILSNGIKEEEQVPSTTELSKLY
                     KINPATVLKGINILVDKNILYKKRGIGMFVSDGAKTIIKEARKENFKHNFVKNLLQEA
                     NKLEINREELVDIIINFKED"
     gene            complement(3871..4530)
                     /gene="deoC"
                     /locus_tag="PC471_003525"
     CDS             complement(3871..4530)
                     /gene="deoC"
                     /locus_tag="PC471_003525"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HDF2790609.1"
                     /translation="MKHILKTVDHTILKATTTWEDIKILCDEAVDMSVASVCIPPSYV
                     KRASEYLKGKIKICTVIGFPLGYQTTATKVFEAKDAIENGADEVDMVVNISDIKNKDY
                     DNIGKEIKEIKKAIGDKVLKVIIETCYLDEDEKIKMCEIVTMSGSDFIKTSTGMGTGG
                     ATLEDIKLMKEHVGKNVKIKAAGGVKSISDAEKFIEAGAERLGTSSICKILKNEDTTD
                     Y"
     gene            complement(4566..5753)
                     /gene="murJ"
                     /locus_tag="PC471_003526"
     CDS             complement(4566..5753)
                     /gene="murJ"
                     /locus_tag="PC471_003526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HDF2790610.1"
                     /translation="MKQEPCDFSHGRFRTGIIFIGITSVMSAFLQIKENFIVVGFGSI
                     PYNIVIIISIMLSTIFGPYILPIGAVVAMVVQLLFYMFFVKKTNYKYLYYLNFKDDSL
                     IKLLALLSPVFIGVAVNQVNSLVDTTLASTLVKGSIPALTYADRLNGFVTGTFTASIV
                     SVMYPMLSKLSAENNQKKFTSSVKSSINMIIISMIPISVASIFFATPVVRIIFERGAF
                     DARATQMTATALIFYAVGMTAFGLRDILGKVFYSLQDTKTPMVNGIISVGVNIVLDLV
                     LIKPMAHGGLALATSSSSIACILLLFLNLKRKVGYFGQDKIIKATLKSVVASLIMGVL
                     SYFTYKFIFGILGVGTFNEFVSLAISVIVGGGIYTLLMIIFKVEEVDMILNIAKRKLH
                     LKK"
     gene            complement(5830..>6192)
                     /locus_tag="PC471_003527"
     CDS             complement(5830..>6192)
                     /locus_tag="PC471_003527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDF2790611.1"
                     /translation="QKLSTKLIKENDIICIEDLQVKNMIRNRKLSRLISDVSWSEFIR
                     QLEYKANWYGRQIVKVGKFFASSQICNKCGYKNEEVKNLNIREWICPSCNETHDRDIN
                     ASINILKEGLRLITIQNK"
BASE COUNT         2053 a          969 c          683 g         2487 t
ORIGIN      
        1 aatagcttgt aaatcttaag attatataca tatatttcta aagattttct taagaaacag
       61 ttagatttta ctaatataag tttattaatt agcattatac taatttatct taatatgatt
      121 ttttgttgat tttaagctgt ttatcgtata aataaaattt ttcttgacta tatagttact
      181 atatagctta ttatatatag agagtaataa aaggaggtaa aatttatgga aaatttattt
      241 acaagaaaat tcactacttt tgaatttcta aaatttgttt ctcctgcaat tatatccatg
      301 atatttatat ctttgtacac aataatagat ggcatctttg tatcgacatt agttggttct
      361 gatgctcttg ctagtataaa tattgtacta cctataatta accttgtttg tggatttgga
      421 ataatgatgg caactggtgg aggtgctatc gtttctatac gtatgggtga aaatagacag
      481 gatgaagcca actctacatt ttcttttata gttttgtttt cattgattgt tgggatttta
      541 ttcacagtaa tctcatattt ctttatcaaa gaaatatcta ttttgcttgg tgcaacagat
      601 aagttattac catattgtat aacttatggt aaagttatga ttttatgtac accattttat
      661 attttaaaat ttatatttga gtactttgca agaactgatg gaaattctaa atttagttta
      721 tttctatcag tcattggtgg tgtaacaaat ataatattgg attatgtatt tattaaatat
      781 tttggaatgg gtcttttagg agctgcagtt gcaactgcta taggtattat tttaacttgt
      841 gttttaggta ttatttactt cttatctaat aaatctacac taaaattaag aaaaccaaaa
      901 accgatttta gacttataag agatactatg ataaatggtt cttctgaaat ggttacagaa
      961 ttatctacag gaattacaac attcttattt aatgtagtag ctttaaaatt agcaggagaa
     1021 aatggacttg ctgctcttac tatagtattg tatgctcatt ttttaatgac atcagtctat
     1081 ctaggattcg ctgctggagt gtctccatta ataagctata attttggtgc tgaaaacagt
     1141 gataaattaa aagaaacatt taaacattct ctaaaattta tatttgtttc ttctctttta
     1201 gtgtttattt ttgctttagt atttgcacca tttattgtta gggtctttgt aagtccagat
     1261 aacacagtat ttaaactagc cttacaagga ttaaaaatat ttgcatttgc ttttttgttt
     1321 gttggtataa atatatttgc atcaggattt tttacagcat ttcacaatgg aaaaatttca
     1381 gctattatat cttttagtcg tgcctttgtt tttataatca taggaatcat aattcttcct
     1441 cctatgttaa acatgactgg attatggctt acagttccat ttgctgaggt tataaccata
     1501 tttatatcta ttctatttat aaaaaaatat aaatgtagat ataagtatta aaaattaata
     1561 cttaaaatca acatttatta aaactaataa acctgcttta aaatacaaat ttacaaattt
     1621 gatttttaag caggttttat tttagttata ataagtcatt tgcatactct ttaattggtg
     1681 cttttctaag taataaaatt ataaatatta tgcaaataac tataattcct acttttgttc
     1741 caaataaaat aaaaggattc tgaaatgaat aaataatttt ttctaaatac aatagcattt
     1801 tatgcatatt tttagttgca aaattaacta tataaattat cattaatatc ggtaatccta
     1861 tcaatatagt aatactagta acttttctaa ctctatatgt aaaagctcca agtaacactc
     1921 ctataaaacc tatacatatt tcatttatac atatttgtac aaaataattt aaataagtta
     1981 gtggcatact tgatgttgaa ggatctatca gtgctgatat agatgtccaa atcatatctc
     2041 tctttagtaa aactgatagt tctatactgt aacctgttat aatttctata aataacttcg
     2101 ataaaaaaat caaaactata cctaaaatag aaatcacaaa gcttaaaata attaaatcta
     2161 tgacaatagc ttttatacaa ctttttctat ctgctcttat acttaaagct cctgaaaagt
     2221 ctttatttgc tatatttact ccatacacaa atagcagtat agttatatat gttaaaatag
     2281 gattcacaat cgtggataaa ttagttttag atgcttctcc aaaaatcttg agaataaaca
     2341 ctggcaaaat attcataaca attcccacaa tcataaatat aattatatac aatttagtat
     2401 ttttattaaa aaattttata tatggcttta attcattcaa ttacaattcc tccttcttag
     2461 tcatgttaac aaacatatct tgtagcccta tgtatcctac atcaatattt gaatttttaa
     2521 gtattttttc atcactttca ctaaaatcac catagtaaaa ataagcaact gcatttccaa
     2581 atgccttttt aggttttata ttttttatac attctaattt ttctaactct tctttatttc
     2641 cagtaatata atgagctttt tgttttatga catcaatttc ttcatctata ataatttttc
     2701 cctcatttaa tattattaca tgctccagta aatcatctat ttcatcaatc aaatgagttg
     2761 atattattat tgtccttgga ttttttatat aactatctaa aattatgtta taaaactctt
     2821 gtctatttac tgcatccaga ccaattgttg gctcatcaaa tatggtgata gctgaattag
     2881 aacaaatacc tattatattt gaaagtatag ttttcatacc tcttgacagt tgtttatact
     2941 tttttttagt atttaaatca aaatatttgc ataattcttc ttctaagcct ttatcataac
     3001 ttttataaaa actagaagaa aattcaaata tctgacctac tttaaaatct ggactataaa
     3061 attctttttc cctaacgata caaacttctt ccaaaacttt tatatcttct tttgggttct
     3121 ttcctaatat ttctatctca ccttcatttt gaattaactg atttaccatt atatttaata
     3181 atgttgtttt tccagctcca ttcttaccta ataaaccata tatcttattt ttttcaaagc
     3241 ttagagacat attattaaaa atattttgct tcttaaaact gtgagataaa ttttctatct
     3301 taatagaact actcatatat taatcctcct tgaaattaat tataatatca accaattctt
     3361 ctctatttat ttctaatttg ttagcttctt gaagtaggtt ttttacaaaa ttatgtttaa
     3421 aattttcttt tctagcttct tttataatag tttttgcacc atctgataca aacatcccta
     3481 tccccctctt cttatataaa atatttttat caaccaatat atttatacct tttaatactg
     3541 tagctggatt aattttataa agctttgaaa gctctgttgt tgatggaacc tgttcttctt
     3601 cttttattcc atttgatagt atctcatctt caattgctcg tgctatctga ataaatatag
     3661 gttcttcatt atttaatact aatttcatag gttcctcctt tctccatcag ttagttactc
     3721 aactaattaa ctatataact tataatatct tgaaaaatat tttctgtcaa tactaaataa
     3781 aaaacagagg gaaaattccc tctgtttttt gttctttata tttataatta aattttgatt
     3841 tttgtaatcc tatcccataa gttaaaaact ttaataatct gttgtatctt cattttttaa
     3901 tattttacat attgagctag ttcctaacct ttctgctcct gcctctataa atttttctgc
     3961 atcagaaatt gatttaactc cccctgcagc ttttattttt acattttttc caacatgctc
     4021 tttcataagt tttatatctt ctaaagtagc tccacctgtt cccattccag tagatgtttt
     4081 tataaaatct gaacctgaca ttgttactat ttcacacatt tttatttttt catcttcatc
     4141 taaataacat gtctctataa tgacttttaa tactttatct cctatagctt tttttatttc
     4201 ttttatttct tttcctatat tatcataatc tttatttttt atatctgaaa tgtttacaac
     4261 catatcaact tcatctgctc cattttcaat agcatctttt gcttcaaata cttttgtagc
     4321 agtagtttga tatcccaaag ggaaccctat cactgtacat atctttattt taccttttag
     4381 atattctgat gctcttttca catatgatgg tggtatgcaa actgatgcta cactcatatc
     4441 tacagcttca tcacataaaa ttttaatatc ttcccatgta gttgtagctt ttaaaatagt
     4501 atggtctact gtttttaata tatgtttcat aaaatcctcc taaatttata attaatataa
     4561 ttagattact tttttaaatg caattttctc tttgcaatgt ttagtatcat atcaacctct
     4621 tcaactttaa aaataatcat tagtagtgta taaattccac ctccaactat aactgaaata
     4681 gctagtgata caaactcatt aaatgttcct actcccaaaa ttccaaatat aaatttatac
     4741 gtaaagtatg ataaaacacc cataattaaa gatgcaacta ccgatttcaa agttgctttt
     4801 attattttat cttgtccaaa atatccaact tttctcttta ggttcaagaa gagtaacaat
     4861 atacatgcaa ttgatgatga actagttgca agagcaagac caccatgagc cattggtttt
     4921 attaaaacta agtccaatac tatatttacc cctacagata taatcccatt taccataggt
     4981 gtttttgtgt cttgtaatga ataaaaaacc tttcccaaaa tatctcttaa gccaaatgca
     5041 gtcataccaa ctgcataaaa aattaaggca gttgctgtca tttgggttgc cctcgcatca
     5101 aaagcacctc tctcaaaaat gattctaaca acaggagttg caaaaaaaat agaagcaact
     5161 gaaataggaa tcatagaaat tattatcata tttatactgg atttaactga actagtaaac
     5221 ttcttttgat tattttctgc acttaactta gataacatag gatacataac agatactatt
     5281 gaagctgtaa atgtccctgt aacaaaacca tttaatctat ctgcataagt aagtgctgga
     5341 atgcttcctt taactaatgt tgaggctaaa gttgtatcta ccaatgagtt cacttgatta
     5401 acagcaacac ctataaatac tggtgaaagt aatgctagta atttaataag gctatcatct
     5461 ttaaagttta agtagtataa gtatttgtag ttcgtttttt taacaaaaaa catgtaaaag
     5521 agcagttgaa ctaccatagc aaccacagca ccaattggaa gtatgtatgg tccaaatatg
     5581 gtacttaaca ttattgaaat aattattact atattatagg gtatacttcc aaatccaact
     5641 acaataaagt tctctttaat ttgtaaaaat gctgacataa cactcgtaat acctatgaat
     5701 attattcctg ttctgaacct cccatgacta aagtcacagg gttcctgctt catagaattt
     5761 tgcatactaa aatatgtctt actaattctc cacaggctat ccccgtagtt cctacggttc
     5821 ttacatacgt tatttatttt gaattgttat tagtcttaat ccttctttta atatatttat
     5881 acttgcattt atatctctat cgtgagtttc attacaacta ggacaaatcc attctcttat
     5941 atttaagttc ttaacttcct cgtttttata cccacattta ttgcatattt gtgaacttgc
     6001 aaagaattta cctaccttta caatttgtct accataccaa ttagccttgt attctaattg
     6061 acgaataaac tctgaccatg atacatcaga aattaagcga gatagtttac gatttctaat
     6121 catattcttt acttgtaaat cttctatgca aataatatcg ttctctttta ttaatttagt
     6181 agatagtttt tg
//
LOCUS       DAMJYR010000186         6144 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.191, whole genome shotgun sequence.
ACCESSION   DAMJYR010000186 DAMJYR010000000
VERSION     DAMJYR010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6144)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6144)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6144
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.191"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            132..1496
                     /locus_tag="PC471_003528"
     CDS             132..1496
                     /locus_tag="PC471_003528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HDF2790612.1"
                     /translation="MDIKELVKMQRKYYNTGKTRDISFRIEQLKKLKLVVSQNEEKIL
                     LALKKDLNKSDFEGFMTEVGMFYSELNFAIKNIRKWSKIKRVKSSMVNFPSISKVVPQ
                     PYGVTLIMSPWNYPFQLALIPLVWSLAAGNCVILKPSEYSVSTSSVVKDIVEDTFSKE
                     YVAVVQGSQEESEKLLLERFDYIFFTGSTNVGKIIMKSASEHLTPITLELGGKSPCII
                     LKDANIDLTAKRLTWGKLINAGQTCVAPDYVLVHEDRKNELIEKIKYYTNKYFGDNAC
                     NNEQFPKIINQRHFNRILSLIDKDKIVYGGNYNKETLKIEPTIVDNVNWDDNIMKEEI
                     FGPIFPILTYKDLDEVIQKIIQMPNPLALYIFTKNKYLENKLLEMIPAGGCCINDTVT
                     HIATNYLPFGGIGESGMGSYHGKAGFDTFTHYKSVLKKLNLDVPIRYAPYDNKLIKVL
                     KKIM"
     gene            1635..2801
                     /locus_tag="PC471_003529"
     CDS             1635..2801
                     /locus_tag="PC471_003529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HDF2790613.1"
                     /translation="MKINWNTKYTTIAIYTFIIAASSIIFYLVSSQIDVFSNNLDAIF
                     TTLQPFIIGFAIAYLLNFILKFYEDRIFIKSEKLKKLKQSSKRGLGLLLTYATATLIL
                     YLFMHFVLPQVIESIVGLANDIPMYVNNATKLIDKLMTDLNLDEQYFNLAVDKWNEFV
                     TYIIKFVTDLIPILGSMLKNVASSIWNIVLGLIVSVYLLIDKEKFYGLSKKITYAVFT
                     EKQAARILELTHRSNYTFGRFLGGKILDSFIIGILTFVILTLVKMPYTLLISVIIGIT
                     NIIPFFGPLFGAIPSTLIVMFVSPIKAFWLLLIILIIQQIDGNIIGPKILGDSIGISA
                     FWILFSLLVAGKLLGFIGMVIGVPMFAVIYSIIKDIVESKLDKKGLPTDTSDYM"
     gene            2918..3700
                     /locus_tag="PC471_003530"
     CDS             2918..3700
                     /locus_tag="PC471_003530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HDF2790614.1"
                     /translation="MAFIECKFKSKYTGGETDIIVILPVANGKELFSGKEIYDFEGKF
                     KTLYLFHGLEDDQSSWIRYTNVEHYAKNKNIALVIPRVETSFYTDMAYGHQYFTFVSE
                     ELPKFVRAIFPLSDKREDNFVAGMSMGGYGALKLALSKPKEFSAVASFSGSPDMIHEL
                     ENQQIETNADILFHDFGRPEDARNSENDLLYILKNLKERNEDIPKIYQFCGDRDFLYK
                     NNQMFKKYADSLGVDVEYEECVGGHEWRLWNLWIKKFIDIIG"
     gene            complement(3819..4472)
                     /locus_tag="PC471_003531"
     CDS             complement(3819..4472)
                     /locus_tag="PC471_003531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDF2790615.1"
                     /translation="MSKSEKGKETKASIISAARELFFEQGYHKTTTRQIAERANINLG
                     LISYYFSSKSEIGAIIYEDIRNEMQSLIYNYHEEGTMMMFFLAMNADLCFLLKNEAYR
                     NFYLDIAYEEAILSYQRSVTEMVMKRYVNDKESIEHDELILSCIGILAMKPEIIRVYT
                     SHKYDISSQAVIKHFTKQFLINLGHSTDLCEEILEELSKYYIDIVDNFTPIITRVRT"
     gene            complement(4496..5860)
                     /locus_tag="PC471_003532"
     CDS             complement(4496..5860)
                     /locus_tag="PC471_003532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HDF2790616.1"
                     /translation="MLLLIVTCAIYIWIGFYFSKKVKTASDFLIAGRNLPLPILAATI
                     AATSFGGGCLVGGVQWGAERGLWVGMYSTIGAALACFINAFFAGKLRSLSCDITPADY
                     IETRYGHSVFLRAYQSLVTPISILAIMGSQLISFGSVSTAFGIPYDIAVIIGVIVIII
                     YTYTSGMWGVAVSAFIQLAICIVFLPIVAYISLKLVGDNPLLMLQSMVKEPFFPGNKS
                     ISDFMYTVLPLVLGSIFSYESFLRYQCADNAKNAVKSSVIAGFILLFLAFPIGIIGAI
                     GGSLFPNISSVQVLPQMISTTLPPLISVLFLAAILAAIMSTADSLMTSMSAIISRDIY
                     NKLLHPNIEFNDLKNTLKYSQIASALTAIIGAIIALKFDSLLELLFWPAPLCTGVIFA
                     PFVIGLCWKGATKKGAIYAVIVSVILALLNMIGILTVFDRILVPIIGGSITIFIVSKF
                     SKKD"
BASE COUNT         2314 a          764 c          933 g         2133 t
ORIGIN      
        1 ggcttttttt attggaagat ttatgttata ttgagatgaa tttattatga tataaaaaat
       61 aatgttagga taaatttaaa tctttttcta aaaatgtatt agtataattc ttaaagaaag
      121 gatttttaac aatggatata aaagagttag ttaaaatgca aagaaaatat tataatacag
      181 gtaaaacaag agatatatcg tttcgtatag aacagttgaa gaaacttaag ttagttgtat
      241 cacaaaatga agaaaaaata cttttagcac taaagaaaga tttaaataaa tcggattttg
      301 aaggttttat gacagaggta ggcatgtttt atagtgaatt aaactttgct ataaagaata
      361 taagaaaatg gagcaaaata aaaagagtta aatcaagtat ggttaatttt ccatctatat
      421 ccaaagttgt tccacaacct tatggtgtta ctctcataat gtcaccttgg aattatccat
      481 ttcaattagc acttatacca ttagtttggt cattagcagc agggaattgt gttatattaa
      541 aaccttctga atattctgtg tcaacatcta gtgtagttaa agacatagtt gaagatactt
      601 tttcaaaaga atatgttgca gttgttcaag gaagtcaaga agaaagtgaa aagttactat
      661 tggaaagatt tgattatata ttttttacag gaagtactaa tgttggtaaa ataatcatga
      721 aatcagcatc agagcattta actcctatta ctttagaact tggaggaaaa agtccttgta
      781 tcattttaaa agatgctaat atagacctta ctgccaaaag acttacttgg ggaaaactaa
      841 taaatgcagg acaaacctgc gtggctccag attatgtttt agtgcatgaa gatagaaaaa
      901 atgagttaat agagaaaata aaatattata ctaataagta ttttggggat aatgcatgta
      961 ataatgagca atttcctaaa atcataaatc agagacattt taatcggata ttatctctta
     1021 ttgataaaga taagatagtc tatggtggaa attataataa ggagacatta aagatagagc
     1081 caactatagt agacaatgtt aattgggatg ataatataat gaaggaagaa atatttgggc
     1141 ctatatttcc cattttaaca tataaagatt tagatgaagt aatacaaaaa attatacaaa
     1201 tgccaaatcc attggcactt tatattttta caaaaaataa gtacctagaa aataaacttt
     1261 tagaaatgat tccagcaggc ggatgttgta tcaatgatac agttactcat atagctacga
     1321 attacttacc ttttggtggt attggtgaaa gtggtatggg ctcatatcat ggaaaagctg
     1381 gattcgatac atttactcac tataagagtg tgttaaaaaa attaaatttg gatgtcccta
     1441 ttagatatgc tccatacgat aataaattaa ttaaggttct taaaaagata atgtaggtta
     1501 tattgtagaa taataaatat aaataaaatt gagtatttgt attctgaata tattttattt
     1561 ttaatttggt atatttgtta taaaagtggt gattttgtga taaaatttaa ttatatagaa
     1621 ataaggagga aaaaatgaaa attaattgga atacaaaata cacaacaata gcaatttata
     1681 catttataat agctgctagt agtattatat tttatctagt atcatcacaa attgatgtat
     1741 tttctaataa tttagatgct atatttacaa cattacaacc ttttataata ggatttgcta
     1801 ttgcatatct tcttaatttt attttaaagt tttatgaaga cagaattttt ataaaaagtg
     1861 agaaattaaa aaaactaaaa caaagttcaa aaagaggctt gggtctttta ttaacatatg
     1921 caacagctac attgatactt tacttattta tgcatttcgt attgccacaa gtaattgaga
     1981 gtattgttgg actagcaaat gatataccta tgtatgtaaa caatgcaact aagcttattg
     2041 ataaattaat gactgattta aatcttgatg agcaatactt taatttggca gttgataagt
     2101 ggaatgaatt tgttacatat attataaagt ttgtaacaga tttaatccca atattaggaa
     2161 gtatgttaaa gaatgtagcc tctagtatat ggaatattgt actaggatta attgtttcag
     2221 tgtacttatt aattgataaa gaaaaattct atggattaag caaaaaaata acttatgcag
     2281 tatttacaga aaaacaagct gccagaattt tagaattaac tcatagaagt aactatacat
     2341 ttggaagatt tttaggtggc aaaatattag attcattcat tataggtatt ctaacatttg
     2401 taatacttac gttagttaaa atgccatata cattattaat atcagttata attggtatta
     2461 caaatattat ccctttcttt ggacctttat ttggagctat accatctacg ctaatagtga
     2521 tgtttgtatc tcctataaaa gctttctggt tattgttgat tatattaata atacaacaga
     2581 tagatggaaa tattatagga cctaaaatac ttggagattc aattggtata tctgcatttt
     2641 ggattttgtt ctccttattg gttgcaggaa aattattagg ttttattgga atggttatag
     2701 gagttccaat gtttgcagta atttattcta taattaagga tattgttgaa agtaaattgg
     2761 acaaaaaggg attaccgaca gatacttccg attatatgta gctaaaaaat attatgtata
     2821 attaaaaata tataaattaa gcaatattta aagcactgag aaagttaaac tcttggtgct
     2881 ttttagatat aaaattatta tagaagaggt gttttttgtg gcatttatag aatgtaaatt
     2941 taaatctaaa tatactggag gagaaactga tataattgta attttgcctg tagcaaatgg
     3001 aaaagaactt tttagtggta aagagatata tgattttgaa ggtaaattta aaacattata
     3061 cttatttcat ggtttagaag atgaccaaag ctcatggatt agatatacta atgtagagca
     3121 ttatgctaaa aataaaaata tagccctagt aataccaaga gtagaaacta gtttttatac
     3181 tgatatggcg tatggacacc aatattttac ttttgtaagc gaggaattac ctaaatttgt
     3241 tagagctata tttcctttat cagataaaag ggaagataac tttgtagctg gtatgtctat
     3301 ggggggatat ggagctttaa aattagcgct ttcaaaacca aaagagtttt cggcagttgc
     3361 aagtttttct ggttcgcctg atatgattca tgagttagaa aatcaacaaa tagaaaccaa
     3421 tgcagacatt ctatttcacg attttggtag accagaagac gctagaaata gtgagaatga
     3481 tttactttac atacttaaga accttaaaga aagaaatgaa gatattccta aaatttatca
     3541 attttgtggt gatagagatt ttttatataa aaataatcag atgtttaaaa aatatgcaga
     3601 ttctctgggt gtagatgttg aatatgaaga gtgtgtaggg gggcatgaat ggcgtctttg
     3661 gaatttatgg ataaagaaat ttatagatat aattggatag tacacttatg gtattaacag
     3721 gtatactata aaatatcttt aaatatgata aagactgaaa tcctaaaatc aaattatgat
     3781 tttaaaattt cagtctctaa gaatttaata aaattttatt aagttcttac acgagttatt
     3841 attggagtaa aattgtcaac tatatctata tagtatttac tcaattcctc taaaatctct
     3901 tcgcataaat ccgtactatg accaagatta ataagaaact gttttgtaaa atgctttatt
     3961 acagcttgag aagatatatc gtacttgtgt gaagtataaa ctctaattat ttcaggtttc
     4021 atagcaagta taccaataca agaaagtatt aattcatcat gttctataga ttctttatca
     4081 ttaacatatc ttttcatgac catttcggtt acacttcttt gatagcttaa aatagcttct
     4141 tcataagcta tatccaaata aaaatttcta tatgcttcgt tttttagaag gaaacaaagg
     4201 tcagcattca tagcaagaaa gaacatcatc atagtacctt cttcatggta gttatagatt
     4261 aaagactgca tttcatttct tatatcttca tatattatag ctcctatttc gcttttactt
     4321 gagaagtaat agcttattag acctaaattt atattagctc tttctgcaat ttgcctagtt
     4381 gttgtcttat gataaccttg ctcaaaaaat aattctctag cagcacttat aatactagct
     4441 tttgtttctt tgcccttttc tgattttgac atatttactg tgtatacctc tcttttcaat
     4501 ctttttttga aaatttgcta actataaata ttgtaataga accacctatt ataggaacca
     4561 aaattctgtc aaatacagta agaataccaa tcatatttaa taatgccaat ataacactta
     4621 ctataacagc atagatagct cctttttttg tagcaccttt ccagcaaaga ccaattacaa
     4681 atggtgcaaa tattacacct gtacataatg gagctggcca aaacaataat tctaataaac
     4741 tatcaaattt aagtgctatg attgcaccta ttatagctgt caaagctgat gcaatttgag
     4801 aatactttag agtatttttt aaatcattaa attctatatt aggatgtaat aatttgttat
     4861 aaatatctct tgatataata gcactcattg aggtcattaa tgaatcggca gtagacatta
     4921 tggcagccaa tatagcagct aagaataata cagatattaa aggtggcaaa gtagtcgata
     4981 tcatttgagg taaaacctga acagatgata tatttggaaa taaactacca ccaatagctc
     5041 caattattcc tatagggaaa gcaagaaata atagtataaa tcctgcaatt acactacttt
     5101 taactgcatt tttagcatta tcagcacatt gatatcttaa aaaactttca taagaaaata
     5161 ttgagcctaa aactaatggt aaaactgtat acataaaatc actaatcgat ttatttcctg
     5221 gaaagaatgg ttcttttacc attgattgta acataagtaa agggttgtcg ccaactaatt
     5281 ttaagcttat ataagctact attggcaaaa acactataca aatagcaagt tgaataaatg
     5341 ctgatactgc aactccccac attcctgatg tataagtata tataatgata actataactc
     5401 ctataattac agctatatca taaggaatac caaatgcagt agatacagaa ccaaaagaaa
     5461 tcaattgact acccataata gctagtatag atattggtgt tacaagactt tgataagctc
     5521 ttaaaaatac actatgacca tatctagttt caatataatc agcaggtgtt atatcacaag
     5581 ataagcttct taattttcct gcgaaaaatg catttataaa gcaagctaaa gctgcaccta
     5641 tagtagaata cattccaacc caaagtcctc tttcagctcc ccattgaact ccaccaacta
     5701 agcaccctcc tccaaaagat gtagcagcaa tggttgcagc tagtataggc aaaggcagat
     5761 ttctacctgc gattaggaaa tcggatgctg ttttaacttt ttttgaaaaa taaaaaccaa
     5821 tccatatgta tattgcacac gtaacaatta aaagtaacat agtcttttct cctttataaa
     5881 atattttaat aaaatataca aaatatattt aaaatatatt ttatataaaa gtagtacgat
     5941 atttgtttta ttggttttag attaatatag ctataataat ttatgatatt agatggaaat
     6001 tacgtaaaaa tagaaaattg aaaattctga aatttttgca tattgatatt tatctcatag
     6061 attctttttt gtaattgtaa cacgctttta tttttaaatc aatagtacat gtataaaaat
     6121 aaaaggaatt taaataagaa aaag
//
LOCUS       DAMJYR010000187         6124 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.192, whole genome shotgun sequence.
ACCESSION   DAMJYR010000187 DAMJYR010000000
VERSION     DAMJYR010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6124)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6124)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6124
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.192"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            92..439
                     /locus_tag="PC471_003533"
     CDS             92..439
                     /locus_tag="PC471_003533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HDF2790617.1"
                     /translation="MHENKKNLLDGSLAQYNDTAVPKIPVFAGNDITSEVYYFKPNQV
                     LNAHRHPNGEQIFVFLKGEGKMKLGEHECDVKNGDTVFVPTGEWHEITNGSNEEMVAV
                     QITKINAGAEYRG"
     gene            complement(589..1089)
                     /locus_tag="PC471_003534"
                     /pseudo
     CDS             complement(589..1089)
                     /locus_tag="PC471_003534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897038.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
     gene            complement(1116..2621)
                     /locus_tag="PC471_003535"
     CDS             complement(1116..2621)
                     /locus_tag="PC471_003535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDF2790618.1"
                     /translation="MNVAIYLRKSRADEEAEHRGEFETLSRHKTTLLKLAREQNLNVI
                     EIKEELVSGESIEYRPKMLELLGEIKNNNYDAVLVMDIDRLGRGNMQDQGLILDTFKK
                     SKTKIITPRKTYDLNDEFDEEYSEFEAFMARKELKLISRRMQRGKIKSIEEGKFIAQA
                     PPYGYDIKYLNNKERVLVINEDKATVVKIIFDMYLKGDGACKISNYLNELGYKTTTGR
                     TWYEKGIRDIIKNKVYCGYIVWNKVERKRNSSRTRGIEEQIEVKGKHEPIISEDIWNK
                     AQNIRKKRINSPTPNNKITNPLAGIVKCGYCNHTLIAKSNTTSNGYEKYMVCTNCKNK
                     ATKLYILENKILEVLNKWLVNYRLSLKDEKDNSKPENTLEHTLINLNKELDTLEKQKE
                     RLHDFLEQGIYDSNTFGERLKILLSRIENAREAINKTNIDIEMQKERNTVKTQIVPQL
                     EKILSEYTEEKEPYLKNILLKSVINYAVYKKEPNQKSDDFSLILYPNIPRD"
     gene            complement(2820..3674)
                     /locus_tag="PC471_003536"
     CDS             complement(2820..3674)
                     /locus_tag="PC471_003536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HDF2790619.1"
                     /translation="MEWIERLNKAINYIEENITKEIEYEQVAKIACCSTYHFQRMFAY
                     MADVPLSEYIRRRRMSLAAVELQNDDKKIIDVALKYGYSSPTAFNRAFKSIHGVAPSV
                     IKKREITTLKAFPPISFKISIKGAEEMNYRIEKKEAFRIVGVSQPLHNELEKNFEIVP
                     QMWQKVALDGTLQKLIPMMDSQLQGVLGISICNNSEEWKYFISVSSTKSIDNTLEEYT
                     VPAFTWAIFSGEGQCPQAIQELEKRIVTEWLPTSGYEYDNGPDIELYLNPDPQNAKFE
                     VWIPVVKK"
     gene            4059..4340
                     /locus_tag="PC471_003537"
     CDS             4059..4340
                     /locus_tag="PC471_003537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2790620.1"
                     /translation="MPNIEKDLDGTTTITYLNDDIGVPTDENGYKISMTILHGKTICH
                     VVSPETILGRKFTLEEKQEKDTIIYGAIEKIMVDRQLQIKNSNKEVASH"
     gene            4490..5338
                     /locus_tag="PC471_003538"
     CDS             4490..5338
                     /locus_tag="PC471_003538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003442793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HDF2790621.1"
                     /translation="MIKSFLMLGQSNMAGRGFISEVTPIYNERIQMLRNGRWQMMTEP
                     INYDRPVSGVSLAASFADAWCCENQEDRIGLIPCAEGGSSLDEWNIDGILFKHAISEA
                     KFAIQSSELTGILWHQGENDSNNGNYKFYYKKLLSIIETLRKELNIPDIPIIIGGLGD
                     FLGKVGFGKSCTEYVFINQELQKFAFEQDNCYFVTATGLTSNPDGIHIDAISQRKFGL
                     RYFEAFHKKKHIMEALANESELIIPSNSKTYTKTEKIYINSMDLALGKISYDEFESKL
                     IKLNDN"
     gene            5390..5911
                     /locus_tag="PC471_003539"
     CDS             5390..5911
                     /locus_tag="PC471_003539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003442791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2790622.1"
                     /translation="MIPLLILGLLKQNPNSYGYELLALMEKKHYKYIVNFTKGSFYYN
                     LQQLEEKKFIEKVENKQSNKNRETHNYIITELGCQEFEKLMYKYGSKTEYINLSFYAT
                     LLFANDYKNEKLIELLETQIKQTQVRISLLNESIENDKTNSHYFNRMLENSRSHHLVN
                     IEWFEKLLNDIRD"
BASE COUNT         2173 a          851 c          813 g         2287 t
ORIGIN      
        1 aattttattc ctattgtgta aagtgagaat actattttct tatattggta taataatagt
       61 aaatctaata tagaaaagag gtatttctag tatgcatgaa aataaaaaaa atctattaga
      121 cggaagctta gctcaatata atgatacagc agttccaaaa ataccagtat ttgctggtaa
      181 cgatataact tcagaagttt attattttaa acctaatcaa gttcttaatg ctcatagaca
      241 ccctaatggt gaacaaatat ttgtgttcct aaaaggagaa ggtaaaatga aacttggtga
      301 gcatgaatgt gatgtaaaaa acggcgatac tgtatttgta ccaacaggag agtggcatga
      361 aattactaat ggaagcaatg aagaaatggt agccgttcaa attacaaaaa ttaatgctgg
      421 agcagaatac agaggataga tatataagta cagttagtac tttaatatct attttattat
      481 ataaacttta atctcaaata taaaaagagt cttttaaaag gctcttttta tatttttgtt
      541 aattttcatt ataaaaacaa gaaaatatct agctaaaact aatatatttt atttaatatt
      601 ttttatagca ttgtttgcaa actcagtatt tacgatatct ttataaggtg cttccttatc
      661 tagttcttta gcttctttca taacagtcat caatttcttc aaaccttcct cttttaatac
      721 aggattttga gaccatgcat ctattgattt atatctatct acaactgttg ttaaatcttc
      781 taaagatata tcttcaaaga aaggttgcat agccttagct acttctttgc tagatgcttg
      841 ttgaatccat aattgacctt tataaacagc atttgtaaat ctttgaatta aatctttatt
      901 gtttttcatg tatgatctcg tagtagagta tgctgtaaaa ggtatttctc cagaatcttt
      961 tcctatagat gtaactatat atccactacc ttctttttcc attgctgttg ctgtaggttc
     1021 aaatgctgtt gtaaagtcac cctctccacc tgcaaaagct ccagccatta caccaaattg
     1081 gtaatcagtc tatttataac atttaaaaca tataattaat ctcttgggat attagggtaa
     1141 agtattaaag aaaaatcatc agatttttga ttaggttctt ttttatagac tgcgtaattt
     1201 attacacttt taagcaatat attttttaga taaggttctt tttcctctgt atattcactt
     1261 aaaatttttt caagttgagg aacaatttgt gttttaactg tatttctttc cttttgcatt
     1321 tcaatatcta tatttgtttt attaatagct tctcttgcat tttctatacg agataagagt
     1381 atttttaaac gttccccaaa tgtattactg tcataaattc cttgttctaa aaaatcatgt
     1441 agtcgttctt tttgtttttc taaagtatcc aattctttat ttaaatttat caaggtatgt
     1501 tctaaagtat tttcaggttt agagttgtct ttttcatctt ttaaagatag tctataattt
     1561 actaaccatt tatttaagac ctctaatatt ttattttcta atatgtatag tttagtagct
     1621 ttatttttac aattagtaca aaccatatat ttctcatatc catttgatgt agtattagat
     1681 ttggctatta aagtatgatt acagtatcca cattttacaa ttccagccaa tggatttgtt
     1741 attttattgt ttggagtagg ggaattaata cgtttttttc ttatattttg tgccttattc
     1801 catatgtctt cacttattat tggttcatgc tttcctttta cctcaatttg ttcctcaatt
     1861 cctcttgttc tactagaatt tcttttcctc tcgactttat tccatactat atatccacaa
     1921 tacactttgt tttttataat atctcttata cccttttcat accaagttct acctgttgtg
     1981 gtcttataac caagttcgtt taaataattg gatattttac atgcaccatc tccttttaaa
     2041 tacatatcaa agattatttt aacaacagtt gctttatctt catttattac aagtactctt
     2101 tctttattat ttaaatattt aatatcatac ccataaggag gagcttgagc tataaattta
     2161 ccctcttcaa tacttttgat ttttcctctt tgcatacgtc tagatattaa ttttaattcc
     2221 ttacgagcca taaaagcttc aaattcagaa tactcctcat caaactcatc atttaaatca
     2281 tacgtttttc taggagtaat aattttcgtt ttagattttt taaatgtatc tagtattaac
     2341 ccttggtctt gcatattacc acgacccaaa cggtcaatat ccatcactaa aacagcatca
     2401 tagttattat ttttaatttc acctagtaat tctagcatct taggtctata ttctatactt
     2461 tccccagaaa caagttcttc tttaatttca atgacattta aattttgttc tcttgctagt
     2521 tttaaaagag ttgtcttatg tctacttaga gtttcaaatt cacctctatg ttctgcttct
     2581 tcatcagctc tgcttttgcg taaataaatt gccacattca ttaaatcaca ccccttgaat
     2641 atattatatt cagtaacaga ttgattatat ttaaaacaca aaaataaacc ttcaaaatac
     2701 aagatatatt ttttaggttt atttttatac agtaaatttt atagtatatt taaaagcttg
     2761 taatctaagt aaatatttat actattaatt ttatgctttc ttcatttttt aattttaaac
     2821 tattttttta ctacaggtat ccatacttca aatttagcat tttgagggtc tggatttaag
     2881 tacagttcaa tatcaggacc attatcatat tcataaccag aagtaggtaa ccattctgtt
     2941 acaatacgct tttctagttc ttgtattgct tgtggacatt gtccttctcc agaaaatatt
     3001 gcccaagtaa aagctggaac agtgtattct tctaatgtat tatctataga ttttgtactt
     3061 gaaacagata taaaatactt ccattcttct gaattattac aaatacttat acctaataca
     3121 ccttgaagtt gactatccat catagggatt aatttttgaa gcgttccatc taaagcaacc
     3181 ttttgccaca tttggggaac aatttcaaaa ttcttttcta gttcattatg aagtggctga
     3241 gatacaccaa ctatacgaaa tgcttctttc ttttcaattc tataattcat ttcttcggct
     3301 cctttaatac taattttaaa actgattgga ggaaaggctt ttaaagttgt tatttctctt
     3361 ttttttataa ctgatggggc aacaccatga atacttttaa atgcacgatt gaatgctgtt
     3421 ggagaagagt atccatattt taaagccaca tcaattatct ttttatcatc attttgtaat
     3481 tctacagctg ctaaagacat ccgtctacga cgaatatatt cagaaagtgg tacatctgcc
     3541 atataagcaa acattctttg aaaatgatat gtagaacaac aagctatttt tgccacttgt
     3601 tcatactcaa tttcttttgt tatgttttct tcaatatagt taattgcttt gtttaatctt
     3661 tcaatccatt ccataaaatc agctccttgt aatttatata atatctattt taaaatagtt
     3721 aatcctctct ttctatgcac taattaacta gcttaataaa gaatgaacat gagaaagtct
     3781 gtaattaact acaaaaatga acaaatagta ttgtttttca cattaaagta tatcacaaat
     3841 tagtatctaa tttttatata agataacagt tttaatttat tatgaaattt ttataatatt
     3901 ttagcttaga catttaatta tatataacat tttatttata gttaactatt tttgaagttt
     3961 acccttaatc aagcaacact tagttattca attataaata atgattgaga ataggacaat
     4021 ttttaagata cataaaatat aaagaggagg ggatttaaat gccaaacata gaaaaagact
     4081 tagatggtac aactactata acatacctca acgacgatat aggtgttcca acagacgaga
     4141 atggatataa aatatctatg acaatactac atggaaagac aatctgccat gtagtatctc
     4201 ctgaaacaat cttaggaaga aagttcacat tagaagaaaa acaagaaaaa gatacaataa
     4261 tatatggagc aatagaaaaa ataatggtag acagacaatt acaaataaaa aattcaaaca
     4321 aagaagttgc tagtcattag cacttcttta ataagtaaag taggacaagt attttttata
     4381 atattattga cttatagtct aattagacta taatataaac atagatagtc taattagact
     4441 actattaaaa ataaatacaa taagaattta agaaaggata ataataataa tgattaaatc
     4501 atttttaatg ttaggacagt ccaatatggc tggaagagga ttcataagtg aagtgactcc
     4561 aatttataat gaaagaatac agatgttacg taatggaaga tggcaaatga tgacagaacc
     4621 tattaattat gacagaccag tttctggggt aagtcttgct gcatcatttg cagatgcatg
     4681 gtgctgtgaa aatcaagagg atagaattgg gctaattcct tgtgctgagg gaggcagttc
     4741 cttagatgaa tggaatatag atggtatact ttttaaacat gctataagtg aagctaaatt
     4801 tgccatacaa agcagtgaat tgacaggaat tttatggcat cagggagaaa atgacagtaa
     4861 taatggcaac tataaatttt attataaaaa attactttct attattgaaa ctcttagaaa
     4921 agaattaaat attccagata taccaattat tattggtgga ttgggagact tcttaggaaa
     4981 agtaggtttt ggaaaaagtt gtactgagta tgtatttatc aatcaagagt tacagaaatt
     5041 tgcttttgaa caagataatt gttactttgt tacagcaaca ggtcttacat ctaatcctga
     5101 tggtattcat attgatgcaa tatctcaaag aaaatttgga ttacgttatt ttgaagcttt
     5161 tcataagaaa aagcatatta tggaagcatt agctaatgaa agtgagttga taataccgag
     5221 taattccaag acatatacaa aaacagaaaa aatatatata aatagtatgg acttggcact
     5281 aggaaagata tcatatgatg agtttgaatc taaacttatt aaacttaatg ataattagat
     5341 aaaatattca ttaatatact aataaatata gattgaaaga ggtgattttt tgataccatt
     5401 attaatttta ggattattaa aacaaaatcc aaattcttat ggatatgagt tattagcatt
     5461 aatggaaaag aagcattaca agtacatagt aaatttcact aaaggttctt tttattataa
     5521 tttacagcaa cttgaagaaa aaaagtttat tgaaaaagtt gaaaataaac aatctaataa
     5581 aaatagagaa actcataact acataattac tgaacttgga tgccaggaat ttgaaaaatt
     5641 aatgtataag tatggttcta aaacagagta tataaatcta tcattttacg caacactatt
     5701 atttgcaaat gattataaaa atgaaaagtt aatagaatta ttggaaacgc aaattaagca
     5761 aactcaagta agaatttctc tattaaatga atccatagaa aatgacaaaa caaattcaca
     5821 ttattttaat agaatgctag aaaattctcg ttctcaccat ttagttaata ttgaatggtt
     5881 tgagaagttg ctaaatgata ttagagattg attttaataa taaaaaaaca tacatatata
     5941 ttttatataa atgtatgata tatagattaa aaataaggca gataattttc aattgaatat
     6001 ctgtcttttt gttatataag atgttaaaag aaacttatac tatgtaagta aaatttataa
     6061 atataaagtt gttcattgtt gaattacaga cacttaagat gaaaagtatg acatttgata
     6121 taat
//
LOCUS       DAMJYR010000188         6034 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.193, whole genome shotgun sequence.
ACCESSION   DAMJYR010000188 DAMJYR010000000
VERSION     DAMJYR010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6034)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6034)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6034
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.193"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            39..1520
                     /locus_tag="PC471_003540"
     CDS             39..1520
                     /locus_tag="PC471_003540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HDF2790623.1"
                     /translation="MKKNRLIAKNISIAFIVLFFFSVFTFFYVGNINRVLEYETNDII
                     TVTIAGWIILSFLFLGIIIYILYSKANSQKTIEKVAYTDFVTGYSNWRKFELDVTNLL
                     KKTSQNNKYAMVIFDIDKFKAINDIYGHKKGNLILKDIADTLNELTDINETFARVSAD
                     NFNILLTYNKKEDIINIIKKIMANNELVNLSFGIYEIKDKDLSVSVYSDRASLAKSSI
                     KNNSDVNFAFFNDKLREKLLFEDKIEKEMEYALESGQFVMYLQPKYNIKLDKFCGSEA
                     LVRWQYTEKEVIYPGDFIPIFEKNGFIRKIDMYILEQACKEIRSLFDKGISPLPISVN
                     FSRVDFFKKDFIENIVNICDRYKIPYSLIEIEITESSMFGDTDTLFNVSRNLQDIGFI
                     VAMDDFGSGYSSVNMLKNIPLNVIKLDRGFFVDDKDVDKSQIVIKSIVSLIKQLGIRV
                     VAEGIETRSQIEMLKKANCDIVQGYYFSKPLPIKEFEKLVYKI"
     gene            1553..4987
                     /locus_tag="PC471_003541"
     CDS             1553..4987
                     /locus_tag="PC471_003541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HDF2790624.1"
                     /translation="MIRAKSLKNVLSIVLIFLFTIISFSDFAFKTNVQEEENSKINLR
                     EISLQSSNAIKIKLDSSLSFIKSVANMVSYFNTIDNSKIVPLLANISKESDFQRISIV
                     SPDGISHTSDGKIINVSKYQYFKKAIKGESNISEVVKSPIDGKDTFVIATPVFNKNNK
                     IIGVLYGSYYNDKLAEFIDITSFNSEGYIDIFESSGQFVLKSSHTNKLIKSSSNIYDI
                     FKKASFDDGYSYKKIYEDTKNNKSGFTSYEYKGENRYATYTPIGVNDWYVISVIPQYT
                     VRRNIDKINQLAFSLIFKVFLLFIMLIIYIIYKNNKSQKDILKANKEISSITNSIPGG
                     VQKCTLGETCNINFLSDGFIELFGYTREEIKTLFNNDFYSLIHKDDVERIKKEIFKQL
                     YKNKTVELEYRIITKNGNTVWVLDKGELITEPDGKEYFCCVTVDITRTKQLEQEMEQK
                     NLELEMISENVLGGIIITEFNDDFTIKYANSGYLSMIGYTREQLENELKYKACNLVFY
                     EDKKKALESIQHQLNTKGIAVYQHRIRRRNGNIIWVSIKGRLIVDDGIEKGIWILNDI
                     TDLKNAEQKLKINEERFRIALKQTSNTIFEYDIKTKSLLNTSKELDKYNIYKITKNVP
                     YSLVESGVIHKDFSDDFINMYENVINGAKKASCVVKMKLPDENYIWNKVTLTTIFDED
                     GNPVRAIGIQEDISKQKEAEIRYNQEGKYHSAILSEAMITYEINVSKDTFIKGNESWQ
                     KPFGIKPDNNYSQMVKTIANSIVHKDNKETFLNTFLCENLLDAYKNNVTEIKLEYRRM
                     NQENKMIWVICTMHLIKDSITNDIKALAYVKDIDKQKNEEIRLKLKAERDTLTGLYNK
                     GTTEKLINDFLNSEEASLGMHAFLIVDIDNFKSVNDNLGHVFGDKVLFEVSKNLKPIF
                     RKDDIVGRIGGDEFIVFLKNIQSFENIKKKAEKICDIFRNSYTENKKTYKISGTVGIS
                     FVPEHGTVFEELYKKADIALYYAKSKGKDKYALYNDCIYQNELENNISDNNIKLADKC
                     EKSKPVLKEILDSIDNYIYIVNPNTYELLFVNNKVSRLDLEIKLGETCYKSLLKSDRP
                     CNNCPLNGLEENKHSKFKIETNTFGKNISIASWINCIDGEYNCLIECIDSSLYKNK"
     gene            5005..5751
                     /locus_tag="PC471_003542"
     CDS             5005..5751
                     /locus_tag="PC471_003542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HDF2790625.1"
                     /translation="MLNSITLILGIIFTIYYFYLKIVFGGISFSEIFLVVGIVLIIYQ
                     IFKKKIKEKKVFYRVLKIIISIFLIVFVVTESLIIFYPKNSLESKSDYLLILGASVKK
                     TTPSTTLKGRLDTALKYLKINDNCYVVVSGGKGSGEKITEAKAMKDYLIKNGIDKNRI
                     IEEDKSTNTYENFKYSKLKIEEHSQGKLSDLKVKIVTTDFHVLRSKILAYRNGYKNTS
                     FYASKSKLSFVPTYYTREFFALWKTIIFDR"
     gene            complement(5700..6008)
                     /locus_tag="PC471_003543"
     CDS             complement(5700..6008)
                     /locus_tag="PC471_003543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HDF2790626.1"
                     /translation="MNFFKENSTFYFATVEDNKPRVRPFGFIMDYEGKLYFGIVKYKT
                     SYKQLLVNPNVEISTTSKVGKWIRIKGIEKTRYEFISSLFIYYLSKMIVFHNAKNSLV
                     "
BASE COUNT         2507 a          598 c          925 g         2004 t
ORIGIN      
        1 ttatattata attgctaaag agagtgaggt gtagctattt gaaaaaaaat agattaatag
       61 caaagaatat atcaatagca tttatagtac tattcttttt tagtgtattt acattttttt
      121 atgtaggtaa tattaataga gtgttagaat atgaaacaaa tgacataata acagttacaa
      181 ttgctggatg gattatactt tcatttttat ttttaggtat tataatttac atattatatt
      241 caaaagcaaa tagtcaaaaa actatagaaa aagtagcata tacggatttt gtaacaggtt
      301 attccaactg gagaaagttt gaattagatg ttacaaattt attaaagaaa acatcacaaa
      361 ataataaata tgcaatggtc atattcgata ttgataaatt taaagcaatt aatgatatat
      421 atggccataa aaagggaaat ttaatattaa aagatattgc agatacttta aatgaattga
      481 ctgatatcaa tgaaactttt gctcgtgtaa gtgctgataa tttcaatata ttattaacat
      541 ataacaaaaa agaggatata ataaatatta tcaaaaagat tatggcaaat aatgaacttg
      601 taaacttatc ttttggaatt tatgaaatca aagataagga tttatctgta agtgtatata
      661 gtgatagagc ttcacttgca aaatcatcaa taaaaaataa tagtgatgta aattttgcat
      721 tttttaatga caaattaaga gagaaattat tatttgaaga taagatagaa aaagaaatgg
      781 aatatgctct tgaaagtggg caattcgtaa tgtatttaca accaaaatac aatattaaat
      841 tagataaatt ttgtggaagt gaagccctag tcagatggca atatacagaa aaagaggtca
      901 tatatccagg tgattttatt cctatatttg aaaaaaatgg atttattaga aagattgata
      961 tgtacatatt agaacaagct tgtaaagaaa tccggtcttt atttgataaa ggaatttcac
     1021 ctttacctat atctgtaaac ttctcaagag ttgatttttt taaaaaagat tttattgaaa
     1081 acatagtaaa tatatgtgat agatataaaa taccatatag tttaatagaa attgaaatta
     1141 ctgaatctag tatgtttgga gatactgata ctctatttaa tgttagtaga aacctacagg
     1201 atattggctt tatagttgca atggatgatt ttggctctgg atattcttct gtaaatatgc
     1261 ttaaaaatat accattaaat gtaataaaat tagatagagg attttttgtt gatgataaag
     1321 atgttgataa gagtcaaatt gttataaaaa gcatagtttc tctcattaag caattaggaa
     1381 taagggttgt tgctgaagga attgaaacaa gaagtcaaat agaaatgtta aaaaaagcaa
     1441 attgtgatat tgttcaagga tattattttt caaaaccatt accaattaaa gaatttgaaa
     1501 aattagtcta taaaatatag tcagcgagtc ttaaatggga ggtggtgatt aagtgataag
     1561 agcaaaatcg ttaaaaaatg tactgagtat tgttttaata tttttattta caataatttc
     1621 tttttctgat tttgcattta aaactaatgt ccaggaagaa gaaaatagta aaattaattt
     1681 aagagaaata tccctacaaa gttccaatgc tataaaaata aaattagata gttctctatc
     1741 ctttattaaa agtgtggcaa atatggtttc ttattttaat actatagata actcaaaaat
     1801 agttccatta ttagctaata tatcaaaaga atctgacttt caaagaatct ctattgtatc
     1861 tcctgatgga ataagtcata ccagtgatgg caaaattatc aatgtgtcaa agtatcagta
     1921 ttttaagaaa gctattaaag gagaaagtaa tatatctgaa gttgtgaaat ctccaattga
     1981 tggaaaagat acttttgtta tagctactcc tgtatttaat aagaataaca aaataattgg
     2041 tgttttatac ggttcttact acaatgataa attagctgaa tttatagata taacaagttt
     2101 taatagtgaa gggtacatag atatatttga atctagtggt caatttgttt taaaatcttc
     2161 acatacaaac aaactgatta agtcaagtag taacatatat gatatattta aaaaagcttc
     2221 ttttgatgat ggttatagct ataaaaaaat atatgaagat acaaaaaata acaagagtgg
     2281 ctttacctca tatgaataca aaggagaaaa tcgttatgct acttatacac ccattggtgt
     2341 aaatgactgg tatgtaattt ctgtgatacc acaatatact gtaagaagaa acattgataa
     2401 aataaatcaa ttagcatttt ctcttatttt caaggtattc ttattgttta ttatgcttat
     2461 aatatatatt atttataaaa acaataaatc tcagaaagat atactaaaag ctaacaaaga
     2521 aatctcatct attacaaata gcattcctgg aggagtacaa aagtgtaccc tgggtgaaac
     2581 atgcaatatt aattttttaa gtgatggatt tatagagttg tttggttata caagagaaga
     2641 aataaaaaca ttgtttaata atgattttta tagtttaata cataaagatg atgttgaaag
     2701 aataaaaaaa gaaattttta aacaacttta caaaaataaa actgttgaat tagaatatag
     2761 gattataaca aaaaatggta atacagtatg ggttttagat aaaggggagc ttataacaga
     2821 acctgatgga aaagaatatt tttgttgtgt aactgtagat attactagaa caaaacaatt
     2881 agaacaagag atggaacaaa aaaatctaga acttgaaatg atatcagaaa atgttttagg
     2941 tggtataatt ataacagaat ttaatgatga ttttactata aaatacgcta actctggtta
     3001 tctttcaatg attggatata cacgtgaaca attagaaaat gaacttaaat acaaagcttg
     3061 taatcttgtt ttttatgagg ataaaaagaa ggctcttgaa agtatacaac atcaattaaa
     3121 cacaaaagga atagctgtat atcaacatag aataagaaga agaaatggta atattatttg
     3181 ggtttctata aaaggaaggc ttattgttga tgatggtatt gaaaaaggta tttggattct
     3241 aaatgatata acagatttaa aaaatgcaga acaaaaactt aaaataaatg aagaaagatt
     3301 tagaattgca ttaaagcaaa catcaaacac tatatttgag tatgatataa aaacaaagtc
     3361 acttttaaat actagtaaag agcttgataa atataatatt tataaaatta ctaaaaacgt
     3421 tccatattct ctagtagaat caggagttat tcataaagat tttagtgatg attttataaa
     3481 catgtatgaa aatgtaataa atggagcaaa aaaagcaagt tgtgtagtta agatgaaact
     3541 tccagatgaa aattatatat ggaataaagt taccttgact actatttttg atgaagatgg
     3601 taatcctgta agagcaattg gtatacagga agatataagc aaacaaaagg aagcagagat
     3661 aagatacaat caagaaggta agtatcattc agctatattg tcggaagcaa tgataactta
     3721 tgaaataaat gtatcaaagg atacttttat caaaggaaat gaaagttggc aaaaaccatt
     3781 tggaattaag cctgacaata actattctca aatggtaaaa actatagcaa atagtattgt
     3841 acataaagac aataaagaaa catttttaaa tacattttta tgtgaaaact tattggatgc
     3901 atataaaaat aatgtgactg aaataaaatt agaatatcga agaatgaatc aagaaaataa
     3961 aatgatttgg gtcatttgta caatgcactt aataaaagat tctattacga acgatataaa
     4021 agcacttgct tatgttaaag atattgacaa gcaaaaaaat gaagaaatta gattaaaatt
     4081 aaaagctgaa agagatactc ttacaggatt atataataaa ggtactacag aaaaattgat
     4141 taatgatttc ttgaactctg aggaagcaag cttaggaatg catgcatttt taattgttga
     4201 tatagataat tttaaatctg tgaatgataa tttaggacat gtatttggag ataaagtatt
     4261 atttgaagtt tctaaaaacc taaaacctat atttagaaaa gatgatatag taggtagaat
     4321 tggtggagat gaatttatag tattcttaaa aaatattcag tcttttgaga atatcaaaaa
     4381 gaaagcagaa aaaatatgcg atatatttag aaattcttat acagaaaata aaaagacata
     4441 taaaatatct ggaacagttg gaatttcatt tgtaccagaa catggaactg tatttgaaga
     4501 actttataaa aaagcagaca tagcacttta ttatgcaaag agcaaaggaa aagataagta
     4561 tgcactttat aatgattgta tctaccaaaa tgaacttgaa aataatataa gtgataataa
     4621 tataaaatta gcagataaat gtgagaaatc caaaccagta cttaaagaaa ttcttgattc
     4681 tattgataat tatatctata tagtgaatcc aaatacttat gaacttcttt ttgtcaataa
     4741 taaggtgtct cgtttggatt tagagataaa attaggagaa acctgttata aatcactatt
     4801 aaaatctgat agaccatgta ataattgccc tcttaacgga ttagaagaaa ataagcattc
     4861 aaaattcaaa atagagacaa atacatttgg taaaaatatc agtatagcaa gttggattaa
     4921 ttgcatagat ggagaataca actgtcttat agagtgtata gattcttctt tatacaaaaa
     4981 taaataaaaa aggactatga agatatgtta aatagtataa ctttgatact tggtataata
     5041 tttacaattt actattttta tctcaaaata gtttttggag gaatttcatt tagtgagatt
     5101 ttcttagttg taggaattgt actaattatc tatcaaatat ttaagaaaaa aattaaagaa
     5161 aagaaggttt tttatagggt gttaaaaata ataatcagca tttttttaat agtatttgta
     5221 gttactgaga gtctaattat tttttatccc aaaaatagtt tagaatcaaa aagtgattat
     5281 ctcttaattt taggagcttc tgtaaaaaag actacaccaa gtacaaccct gaagggtagg
     5341 ttagacactg cattgaaata cctaaaaatt aatgataatt gttatgtggt agtatctggt
     5401 ggtaaaggaa gtggagaaaa aataactgag gcaaaagcta tgaaagatta tcttataaaa
     5461 aatggtatag ataaaaatag gataatagaa gaggataaat ccacgaatac ttatgagaat
     5521 tttaaatatt caaaattgaa gatagaagaa catagtcaag gaaaactaag cgatttaaaa
     5581 gtaaaaatag taacaacaga ttttcatgtt ctcagaagta aaatattagc atatagaaat
     5641 ggatataaaa atacgtcttt ttatgctagt aaatcaaaac tttcatttgt accaacttac
     5701 tatacaagag aattttttgc attatggaaa acaatcattt ttgatagata ataaataaaa
     5761 agactagata taaactcata tctagtcttt tctattccct taattctaat ccattttcca
     5821 actttacttg ttgtagaaat ttcaacattg ggatttacaa gtaattgctt ataagaagtt
     5881 ttatatttta cgattccaaa atataatttt ccttcataat ccattataaa tccgaatggt
     5941 cttactcttg gtttattatc ttcaactgta gcaaaataga atgttgagtt ttctttaaaa
     6001 aaattcatta ttttttcatg attaaatcct cttt
//
LOCUS       DAMJYR010000189         6022 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.194, whole genome shotgun sequence.
ACCESSION   DAMJYR010000189 DAMJYR010000000
VERSION     DAMJYR010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6022)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6022)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6022
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.194"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(1007..1558)
                     /locus_tag="PC471_003544"
     CDS             complement(1007..1558)
                     /locus_tag="PC471_003544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2790627.1"
                     /translation="MSINTVIAKNLNRLRNERNLSLGQLAELSGVSKVMLSQIEKGDS
                     NPTVNTIWKIASGLNVPYTAILEQPQNETFIVSKTDIDVQVSENKDYRLYCYYPNTPT
                     RNFELFQMELEEGHSYTSVGHSEKSQEYIMIIEGQLKLEVNDSIYQLRENDSICFSAE
                     SIHTYHNQGEKTLKAVIINYYPV"
     gene            1749..2711
                     /locus_tag="PC471_003545"
     CDS             1749..2711
                     /locus_tag="PC471_003545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HDF2790628.1"
                     /translation="MRVLNNVTEAIGNTHMLHLSKIADEFGVQGNIYAKMEYLNPGFS
                     KKDRVALQMITEAENAGLLVPGQAVVELTSGNTGTGLAIACACKGYKFIACMSKGNSM
                     ERARMMKALGAEVVLVDQMPNSVHGQVSGEDLALVEIEAQKIAKERNAFRADQFNLEA
                     CNHAHEKYTAEEMWEQLDGNIDVFVDFLGSGSTFAGCSKTLKKHNSSIKCYVVEPETA
                     AIYSGKEITDQGHKIQGGGYSMDLPLVDKKLIDGYIQVTDEEATDVARKLAKLEGVFA
                     GFSSGANVCAAIKLLKSSEKGKNIVLTLNDSGLKYLSTDLYEEL"
     gene            3006..4850
                     /locus_tag="PC471_003546"
     CDS             3006..4850
                     /locus_tag="PC471_003546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HDF2790629.1"
                     /translation="MQKRILRNFIFTGKNILSKLVFIIKYNFSTLILFELFHKGLALF
                     FIWPSIRYIFDILIKSLHIVYLNMDNFIKVLTNPMSIVLIILSVIILAFYAFFEFTSV
                     IICFNKSIKYEKIGLFELFKISFKNSIKLLYPKNILLFIFVILIIPLVNTVLISGFIG
                     KIQIPEYILDYIKSDIILNIIYMIFLLIIYISVIRWIFSIHEITLNTENFKRARKESV
                     NLIKGKIIRTIIYSLILSLVVAIIGYAIYHSGIIFIGIWTKYCTDMEFLKNVFINRCI
                     AFEEYFRLILSIFIFILDIGFISATYYQYKGVNISKNNIKKEKKSISKIVFNLVLILL
                     ISYIESVAFSSHINGMFNTSFSFYTTAIAHRAGAEMAPENTLAAIEEASHAKADYAEI
                     DVQETKDGELIVLHDSNFKRTTGVDKNVWDVNYNEVKTYDAGSFYYYGKGYYDEKFVG
                     EKIPTLKEMIKYSKGKVKLLIEIKLNGHEKGDVVKKVVQLIKENNFENQCIVASMDKT
                     ILQKVKKLDENLLTCYFMAIAYGDFSKLNYVDIYGIESTFVNKTVVEKIHKMNKKIFV
                     WTVNSDSLIEKMLDLNVDSIITDNPYLIESAIYWKKNGFISQVSDYLF"
     gene            5172..5756
                     /locus_tag="PC471_003547"
     CDS             5172..5756
                     /locus_tag="PC471_003547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HDF2790630.1"
                     /translation="MTEREKMLAGELYDCGDIELLTQWHKAKGLVRDYNQTDSSDLEK
                     KEKILNELLGGKGKNLWITAPYYVDYGNNIYFGNNCEVNMNCTFLDDNKIIIGDNALI
                     APNVQIYTAFHPTNAGDRFGNAKEDGSFEFCKTQTAPVTIGDNVWIGGGAIIMPGVTI
                     GDNVVIGAGSIVTKDIPSNMIAYGNPCRIIRENK"
BASE COUNT         2352 a          591 c          927 g         2152 t
ORIGIN      
        1 aatagattga aatttatgaa tattcatact ataatttaaa tataaggaga tgccctttga
       61 aaataaaaat taaaaaatat ttaatgctac tacaatagga actagaacta cacttaataa
      121 atatacagaa attagatttg gctcaatata aaatacaaat atagaattta ggtgtttttt
      181 atgaaaaaat ttttatatgc tttatatagt ttcattgtta ttatagctaa ttttagatta
      241 aaagaaaaaa attataactt tattttatta gcaatctttt ttatattgct gttaattcaa
      301 aatggagttt taaaaacagg ttatcaaaat agagctacat tttatcaaga taattatttt
      361 aatagagagg ataaaaatga taagataaaa tttatgacta taaagatttt tatatttttg
      421 tcattaccat tatgttttaa tatactattc aattatttaa cctcttaaat tatatttaga
      481 tataggagtc ttatgataaa agtaagctta tgtgttattt gtagtaaata taaagtgcat
      541 atagtgataa aacaaaagtt tcatataaat gaagaaaaaa atataaaata atttatttaa
      601 ctacttaaaa ataaactaaa agggataata aaatagcttt taggttatgg taataaaggt
      661 atttaaaaag agtgcataag cactccaatt gtgagaaata catacacaaa tcattattac
      721 aacaatgaaa aatgtacata taagagacgt tttgtatttg ttttaagtga taatatttta
      781 atactaacta aataattatc acaatagtaa aaaagaaaaa ggagtatttt gtactccatt
      841 tgtcaaaaat ataaaacttt tatataagct tattataaca taattgataa aagtgtaaag
      901 atatataatg aatttataaa gaaaaataaa ataaaaaaga taaatcctaa tcaaaggatt
      961 tatctttttg tatagaaaat aattattcta ttattaaatg gcatggctaa actggataat
     1021 aatttattat tactgctttc aatgtttttt ctccttgatt atggtatgta tgaattgatt
     1081 cagctgaaaa acaaatagag tcattttctc taagttgata aatagagtca tttacttcta
     1141 attttaattg accttctata atcataatat attcctgtga tttttcagaa tgaccaacag
     1201 atgtataact atgaccttct tctaattcca tttgaaataa ttcgaagttt ctagttggtg
     1261 tattgggata ataacaataa agacgataat ctttattttc tgaaacttgt acatctatat
     1321 cagtttttga tacaataaaa gtttcatttt gaggttgttc cagtattgca gtatatggaa
     1381 catttagtcc acttgcaatt ttccaaattg tgtttactgt tgggtttgag tctccctttt
     1441 caatttgcga aagcattact ttacttacgc cagataattc agcaagttga cctagactta
     1501 agttacgttc attacgtaat ctatttaaat ttttagcaat aacagtatta atactcatag
     1561 tagctcctta aaatgtaaat tattgacaaa tataatttac gaatgtataa tttatttaac
     1621 attatttata atttacaaat gtataatata atgaacgtta aatacactgt acatccgtaa
     1681 tataaattat actgcataaa gagttgtttt tcaacggatg atagttgaga gattaagggg
     1741 gaattgtaat gcgtgtactt aataatgtta cagaggctat aggcaatact catatgttac
     1801 atttatccaa aatagcagat gaatttggag ttcaaggtaa tatttatgca aagatggaat
     1861 acttaaatcc gggatttagt aaaaaggacc gtgtagcatt acaaatgatt accgaagcag
     1921 aaaatgcagg tttattagtt ccaggtcaag cagttgtgga attaacaagt ggtaatactg
     1981 gaacaggact agcaatagct tgtgcatgta aaggatataa atttatagcg tgtatgtcaa
     2041 aaggaaattc tatggaaaga gcaagaatga tgaaagcttt aggtgctgaa gtagttcttg
     2101 tagaccaaat gcctaattca gttcatggac aggtatctgg tgaagatttg gcacttgtag
     2161 aaattgaggc tcaaaaaata gcaaaagaaa gaaatgcatt tagagcagac caatttaatt
     2221 tagaagcttg taatcatgca catgaaaagt atacagcaga agaaatgtgg gaacaattgg
     2281 atggaaatat tgatgtattt gtagattttc ttggaagtgg tagtactttt gcaggatgtt
     2341 caaaaacact taaaaaacat aattcttcta tcaaatgtta tgttgttgaa cctgaaacag
     2401 cagcaattta ttcaggtaaa gaaataactg accaagggca taaaatacaa ggtggaggat
     2461 attctatgga tttgccatta gtagacaaga aacttattga tggttatatt caagttacag
     2521 acgaggaggc aactgatgta gcaagaaaat tagctaaatt agaaggtgta tttgcaggat
     2581 tttcttcagg ggcaaatgta tgtgcagcaa ttaagttgtt aaagagcagt gaaaaaggaa
     2641 aaaatatagt tttaactctt aatgatagtg gtttaaaata tttaagtact gatttatatg
     2701 aagaattgta ataatagcac aatagttatg caatgttttt taaattaagt atatagattt
     2761 aaattataaa aagtatctca atgtaatttt tgaaagttat aaaggagata cttttttatt
     2821 ttgtaaatat ggagtatcct acttataagt attaagctat atagatattg atgattagct
     2881 atgcaataaa aaagagtatg tgtttttgca tttatacatc ataacttgga ataatacata
     2941 ttaatgttct aaatgtattt aaaacatatg ataaaattag aacaaataga tacaaggaga
     3001 taaaaatgca aaagagaatt ttaagaaatt ttatatttac gggtaaaaat attttaagta
     3061 aacttgtttt tataataaaa tataatttta gtacattaat tttatttgaa ttatttcata
     3121 aaggattagc cttatttttt atatggccat ctattagata tatatttgat attttaataa
     3181 aaagtttaca tatagtctac ctaaatatgg ataattttat aaaagttttg accaatccca
     3241 tgtcaatagt tctgataata ctatcagtta taatactagc tttttatgca ttttttgaat
     3301 ttacctctgt aattatttgt tttaataaat cgataaaata tgaaaaaata gggttatttg
     3361 aactctttaa aatatcattt aagaactcta taaaattgtt atatcctaaa aatatactat
     3421 tatttatatt tgtgatatta ataattccat tagtaaatac agttttaatt tcagggttta
     3481 taggaaaaat acaaatacca gagtacatac tagattatat aaagtctgat ataattttaa
     3541 atataatcta tatgattttt cttttaataa tatatatttc ggttattaga tggattttta
     3601 gcattcatga gataacttta aatacagaaa attttaaaag agcaagaaaa gaaagtgtta
     3661 atcttataaa gggaaagatt ataagaacaa ttatttattc attaatatta tctttagtag
     3721 tagctattat tggatatgcg atttatcata gtggaattat atttataggc atatggacta
     3781 aatattgtac tgatatggaa tttttgaaga atgtatttat aaatagatgt attgcatttg
     3841 aagaatattt tagattgatt ttatcaatat tcatttttat attggacatt ggatttatct
     3901 cagcaactta ttatcaatat aaaggagtaa atatttctaa aaataatatt aaaaaagaga
     3961 aaaaatctat atcaaaaatt gtttttaatt tagtattaat attattgatt tcttatatag
     4021 agtcagttgc attttcttca catataaatg gtatgtttaa tacgtcattt tcattttata
     4081 caacagcaat tgctcataga gcaggagctg aaatggctcc agagaatact ttggcagcaa
     4141 tagaagaagc ttcacatgct aaagctgatt atgcagaaat agatgttcaa gaaactaaag
     4201 atggagaact tattgtactt catgattcaa actttaagag aactacagga gtagataaaa
     4261 atgtttggga tgtaaattat aatgaagtaa agacatatga tgcgggaagc ttttattatt
     4321 atggaaaagg ttattatgat gaaaagtttg ttggggaaaa gataccaaca ctcaaagaaa
     4381 tgataaaata ttctaaaggt aaggtgaaat tattaataga aataaaactt aatggacatg
     4441 aaaagggaga cgtagtaaaa aaagttgttc agttaattaa agaaaataat tttgaaaatc
     4501 aatgtatagt tgcatctatg gataaaacta tattacaaaa agtaaaaaaa ctggatgaga
     4561 atttattgac atgctatttt atggcaattg catatggtga tttttcaaag ttaaattatg
     4621 ttgatatata tggaattgaa tcaacttttg ttaataaaac agtagtagaa aagattcata
     4681 aaatgaacaa aaaaatcttc gtttggactg ttaatagtga cagtttaatt gagaaaatgc
     4741 ttgacctaaa tgtagatagt attattacag ataatccata ccttatagag agtgcaattt
     4801 attggaagaa aaatggattt attagtcaag tgtcagatta tttattttaa atattctata
     4861 attgtattat ccagtaaaag agatattatt aaaatcaata atatctcttt ttaacataaa
     4921 taaggttgtt aaattaaggt tattcttata aaaattgtgc ttatcagcta taaacaagga
     4981 ataaaagtga aaaaattgct ctgagattat tacagttaaa agagttttaa gatgacagag
     5041 agatagattg tcaactgtat atttaataaa tcttgttatt tattaaattt tatataagta
     5101 aaatatataa attaataaat cttatgctag aatatatatg aaaaacaata taaatttgga
     5161 ggattaaaaa catgacagag cgagaaaaaa tgttggcagg agaactttac gattgtggtg
     5221 atattgagtt gttaactcaa tggcataaag caaaaggcct agtaagagat tataaccaaa
     5281 cagattcctc ggatttggaa aaaaaagaaa aaatattaaa tgagttactt ggtggtaagg
     5341 gtaaaaactt atggattact gcaccgtatt atgtagatta tggaaataat atttattttg
     5401 gaaataattg tgaagtcaat atgaattgta cgtttttaga tgataataaa attatcattg
     5461 gggataatgc actaatcgca ccaaacgtac aaatatatac tgcatttcat ccaactaatg
     5521 ctggtgatag atttggtaat gccaaagaag atggttcatt tgaattttgt aaaactcaaa
     5581 cagctccagt tactattgga gataatgttt ggattggcgg gggtgctatt atcatgccag
     5641 gtgttactat tggagataat gtagttatag gtgcaggaag tattgttaca aaagatattc
     5701 caagtaatat gattgcatac ggaaatccat gccgtattat tcgagaaaat aaataaagtt
     5761 aaattttctt gcaaaatctc aaaaaacacc tttcttactt tatacatagg gtattttcta
     5821 atactttcac atccatattc atttgttaaa tttttgtgga atgtagtttt tatcacttct
     5881 atcttacatt tccaccattt ttttataaaa gtcattcctt tatagattta tttataatag
     5941 tatatattct atttgatgtt tgaataagca cttattttta agtaaaatac taacttttag
     6001 gttagtattt tgctatagta tg
//
LOCUS       DAMJYR010000190         6019 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.195, whole genome shotgun sequence.
ACCESSION   DAMJYR010000190 DAMJYR010000000
VERSION     DAMJYR010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6019)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6019)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6019
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.195"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            <1..410
                     /locus_tag="PC471_003548"
     CDS             <1..410
                     /locus_tag="PC471_003548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4004 family protein"
                     /protein_id="HDF2790631.1"
                     /translation="IANMFMKKDINKEFDIDFILSKEVICNYTKEIFQNLYKKEQSIG
                     KKELLILSIIEKFLVKSIITFEELKLIVNIIEENFTSIYNESGKIYLFRKFGVPFVVG
                     CLDYKKVKFEKDVVKIIEVDLTKEINDISIKLS"
     gene            complement(710..1297)
                     /locus_tag="PC471_003549"
     CDS             complement(710..1297)
                     /locus_tag="PC471_003549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HDF2790632.1"
                     /translation="MNLKDSKTKENLLRAFAGESQARNRYNISASVAKKEGLYIIESL
                     FNYTADQEKSHAKQFFSKLKEFAGENISISGAYPVDLYDKTAQHLKAAQHNELQEWDD
                     VYKNFGKTAREEGFESIAKLFENIAEIEKVHGDRFGRYATDLEEGTLFRRNEDIQWIC
                     THCGHIHIGKEAPKACPVCLHPQGYFLDFKNSLFE"
     gene            complement(1383..1820)
                     /locus_tag="PC471_003550"
     CDS             complement(1383..1820)
                     /locus_tag="PC471_003550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDF2790633.1"
                     /translation="MEDKYIVHFISKTKASMIKFIENKLSKNGLGELIPTHGNILTAL
                     YENNGILTMKEIAAKIGKDKSTVTVLVNKLINLGYLERQKCTNDKRITYIKLTKKALL
                     IEDTFNSISTQVKETAYHNITENEKQEFLRILKKINDNFKNAD"
     gene            1924..2922
                     /locus_tag="PC471_003551"
     CDS             1924..2922
                     /locus_tag="PC471_003551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdH/YhfP family quinone oxidoreductase"
                     /protein_id="HDF2790634.1"
                     /translation="MNKKFKSLYITEEEGNFRRNIVEREVDTLPEGEVLIKVKYSSLN
                     YKDALSANGNKGVTRKYPHTPGIDASGIVESSSCDDFKIGEEVLVTGYDLGMNTSGGF
                     SEYIRVPSSWVVKLPKNLSLKEAMDYGTAGFTAALSVYKFIDSVDKNMGDVLVTGGTG
                     GVAVIATKILIKLGYSVVVSTGKLEEQKEVLLNLGVKDVIHRSEVDDNSGRPLLRPRW
                     AGVIDTVGGNTLATAIKTTNYCGAVTTCGNAGGVDFTSSVYPFILKGITLYGIDSVDC
                     PMNLRLKIWDLLSNEWKLDDLDDMVNVVSLEELNDSIDSIMAGKHVGRTVVDLEKI"
     gene            complement(3042..3968)
                     /locus_tag="PC471_003552"
     CDS             complement(3042..3968)
                     /locus_tag="PC471_003552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HDF2790635.1"
                     /translation="MSKENKNVRIAVVQASPVIMDLEKTVEKALGLIKEAGRKGANIV
                     VFPEAFIPAYPRGLSFGFVVGSRTMEGREDWKRYYDNSVPVPSATTDLLGKAAQEAGV
                     YLSMGITERDGNDINCTLYCTNLFFSPEGKLIGKHRKLKPTGTERCIWGEGDGSTLTV
                     VDTPYGKMGSLICWENYMPLARTALYAKGVKLYIAPTADSREEWQATMKHIALEGRCF
                     VIGCNQYVEKNMYPTDLNYYKELDAEPEIMCPGGSCIVDPFGKYVAGPIFNKEEMLIA
                     DLDLEKIVLSRLDFDSEGHYSRPDVFELIVHE"
     gene            4353..5957
                     /locus_tag="PC471_003553"
     CDS             4353..5957
                     /locus_tag="PC471_003553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HDF2790636.1"
                     /translation="MKEKIYYNGNIITMEDSICGDAILIKDKIIKKIGTKEEVFALKN
                     KDTEIIDLQGKTLMPSFIDSHSHLIAFATTLKLVPLEDATSFKDIVKKIQDFKESNNI
                     KKGDWIIGFSYDNNFLEENKHPDKSVLDSASSENPILISHASGHMGVANTLGLKQLGV
                     TNETRDPEGGHIGRVEGSEEPNGYLEENAFFNVASKIKQPSSNEIFNSIEKAQNIYLS
                     YGITTAQEGLMEENQFNILKAMANQNKLKMDVVGYVNLKKSKSVADNNREFIKKYINR
                     FKIGGYKIFLDGSPQGKTAWLSRPYENSDDGYCGYPIYKDEEVEKFIDISLKEKMQLL
                     THCNGDAAADQLIDAFEKVLNLKEQSSENHIRPVMIHAQTVREDQIDDMKVINMIPSY
                     FVAHTYYWGDIHIKNLGEDRAFKISPLKTTIEKGLIYTLHQDTPVIAPNMLETVWCAV
                     NRITKKGIQIGENEKISPLDALKGVTINAAYQYFEEDKKGSIKEGKLANLIILDENPL
                     TIDPMKIKDIKVLQTIREGEVLYSLK"
BASE COUNT         2238 a          755 c          944 g         2082 t
ORIGIN      
        1 atattgcaaa tatgtttatg aaaaaggata ttaataaaga atttgacatt gattttattc
       61 tcagtaaaga agttatatgt aattatacca aagaaatatt tcaaaattta tataaaaaag
      121 aacaaagcat aggtaaaaaa gaactgctta tattgagcat aatagagaag tttttagtta
      181 aatctataat aacttttgaa gagttaaaat tgatagtgaa tataatagaa gaaaatttta
      241 catcaatata caatgaaagt ggaaagattt atttgtttag aaagtttgga gttccattcg
      301 tagtaggatg tctggattat aaaaaagtaa agtttgaaaa agatgtagtt aagattattg
      361 aggttgactt gacaaaagaa ataaatgata taagtataaa gttatcatag tgtattatct
      421 tatataatgt attataataa tctacttaaa atacacttta tttaaatatt tagtttaaag
      481 atgagatttt atgtgagtgt gtaaaagtta aagatagatt taagtaggtt atagggatta
      541 gtaaacaata aaatgaacca tgatgaacta ctataaattg tatttaaact acattttcta
      601 cttatagtaa tatcaatggt tcattttatt gttattaaga taatacagta tttataaaaa
      661 tacttgatat aaatatagta aattgtttaa atagaattat tgccttaatt tattcaaata
      721 atgagttttt aaaatctaag aaatatcctt gtggatgaag acatacagga caagctttag
      781 gagcttcttt acctatatgt atatggccac aatgtgtaca tatccattgt atatcttcat
      841 ttcttctgaa taaagtacct tcttctaaat cagtagcata tctaccaaat ctatcaccat
      901 gaactttttc tatttcagct atattttcaa ataacttagc tatagattca aatccttctt
      961 cacgtgctgt ttttccaaag tttttatata catcatccca ctcttgaagc tcattatgtt
     1021 gtgcagcttt aaggtgttgt gctgtcttgt catataaatc tacagggtat gctcctgata
     1081 tacttatatt ttcaccagca aattctttta atttagaaaa gaattgtttt gcatgtgatt
     1141 tttcttggtc agcagtgtaa ttaaataaag actctattat atataatcct tctttttttg
     1201 ctacagatgc agaaatattg tatctatttc tagcttgact ctcacctgca aaagctctta
     1261 ataaattttc tttagtttta ctatctttta aattcatatt ctacctctct atcacaataa
     1321 atttttacaa tatttgattc tttttattta ttattccaat aggagatata attatgctca
     1381 atttagtcag catttttaaa attatcatta atttttttta aaattctaag aaattcttgt
     1441 ttttcatttt ctgtaatatt atggtatgcc gtctctttaa cttgtgttga gatgctatta
     1501 aaagtatctt ctataagcag tgcctttttt gtaagcttta tatacgtgat acgtttgtca
     1561 tttgtacatt tttgtctttc taaatatcct aggtttataa gtttattaac caacactgta
     1621 acagtggatt tatcttttcc tattttagct gcaatttctt tcattgtaag tattccatta
     1681 ttttcataaa gtgcagttaa aatatttcca tgagtaggta ttaactcacc taaaccattt
     1741 ttactgagtt tattttcaat gaatttaatc atactagcct ttgttttact tatgaaatgt
     1801 acaatatatt tatcttccat aaattattct ccaatcttta atctatttaa attagtttga
     1861 cataaaacta tttataaact ataattagtt taacataaaa ctaaatgtat tgggaggaaa
     1921 tttatgaata aaaaatttaa atcactatat attacagaag aagaaggaaa ttttagaaga
     1981 aatatagttg aaagagaagt ggatacattg ccagaaggcg aagttctaat aaaagtaaaa
     2041 tattcttctc taaattacaa ggatgcactt tctgcaaatg gtaataaggg tgttacgaga
     2101 aagtatcctc atacaccagg aattgatgca tctggtatag tagaatctag tagttgtgat
     2161 gattttaaaa ttggagaaga agttttagta acaggttatg acttgggaat gaatacttct
     2221 ggtggttttt cagaatacat aagagttcct tctagttggg tagttaaact tcctaaaaat
     2281 ctttcactaa aagaagcaat ggattatggt acagcagggt ttacagcagc tttatctgtg
     2341 tataaattta tagattctgt agacaagaat atgggagatg tattagtcac tggtggtact
     2401 ggaggagttg ctgtaattgc aactaaaata ttgattaaat taggttattc agttgtagtt
     2461 tcaacaggaa aattagaaga acaaaaagaa gtattactaa acttaggtgt taaagatgtt
     2521 atacataggt cagaagtgga tgataactca ggaagacctc tgcttagacc aagatgggct
     2581 ggagtaatag atacagtagg aggaaatact ctagcaacag caataaagac aactaattat
     2641 tgtggagcag taacaacttg tggtaatgca ggaggagttg attttacaag ttcagtatat
     2701 ccatttatct taaagggaat tactttatat ggtatagatt ctgtggattg cccaatgaac
     2761 ttaagattaa aaatatggga tttattatca aatgaatgga aattagatga tttagatgat
     2821 atggtaaatg tagtatcatt ggaagagtta aatgatagta tagattcaat aatggcagga
     2881 aaacatgttg gaagaacagt agttgattta gaaaaaatat aggtgaataa atttataatt
     2941 aggattatag gttttaagca aaaatgagat acttgaaatt tacttgtgag tatctcattt
     3001 ttttatgtaa aaagatttat gtagatgttt gtgtaaaaag attattcatg aacaattaat
     3061 tcaaatacat ctggtcttga atagtgacct tctgaatcaa aatctagtct actaagtact
     3121 attttttcta ggtctaaatc agctattagc atttcttctt tattgaaaat aggacctgct
     3181 acatattttc caaaagggtc aactatacaa cttcctcctg gacacataat ttctggttct
     3241 gcatctagct ctttatagta gttaaggtca gttggataca tatttttttc aacatattga
     3301 ttgcatccaa ttacaaaaca tcttccttct aaagcaatat gcttcatagt tgcttgccac
     3361 tcttctcttg aatctgcagt aggggctatg taaagtttta cacccttagc ataaagagct
     3421 gttctagcta atggcatata attttcccaa caaatcaacg aacccatttt tccatatgga
     3481 gtatcaacta ctgtaagagt acttccatct ccttctcccc aaatacatct ttctgttcct
     3541 gtaggtttaa gttttctgtg tttaccaatt agcttacctt ctggagagaa aaataagttt
     3601 gtgcaataaa gggtacaatt tatatcatta ccatctcttt ctgttatacc cattgataga
     3661 tatactcctg cttcttgtgc tgcttttcct aataaatctg ttgtagcact aggaactggt
     3721 acagaattat catagtatct tttccaatct tctctacctt ccattgttct acttccaaca
     3781 acaaatccaa atgaaagacc tcttggatat gcaggtatga atgcttcagg gaaaactacg
     3841 atatttgccc cttttctacc agcttcttta attaatccta atgctttttc aacagttttt
     3901 tctaaatcca ttatgactgg tgacgcctgg acaacagcaa ttcttacatt tttattttcc
     3961 ttgctcataa aaacctccta aaaaatattt aaattttttg cattgaatag attacatgag
     4021 atgttatttg agaactaaga tacttatatt gtgaataata atgtaactgt atatttaaaa
     4081 taatacatca tgatttaaaa aattacaatt gtacagatat aattgagttt agatatttaa
     4141 atttaacaac ttataatatt ttttcaatta gtataaagcc tattaatttg tcttatttac
     4201 aatgaatagt atttacttct tggcaaacaa taattaaaaa taaaactttt atatgattaa
     4261 actctaaaga aaaagtttat aaagaagata atataattaa gcagaaaata atgaaaaaaa
     4321 tttattaaag tagacaacgt aaggagaatt ttatgaaaga aaaaatttat tacaatggaa
     4381 atatcatcac aatggaagat tcaatatgtg gagatgctat tcttataaaa gacaaaataa
     4441 taaaaaagat aggaacaaaa gaagaagtat ttgctttgaa aaataaagat acagaaatta
     4501 tagatttaca aggtaagaca ttgatgccat cttttataga ttcacatagt cacttgatag
     4561 cttttgctac tactctaaag ctagttcctt tagaagatgc aaccagtttt aaagacatag
     4621 taaaaaaaat acaggatttc aaagagtcga ataatataaa aaaaggagac tggataattg
     4681 gattttcata tgataataat tttttagaag aaaataagca tccagataag agtgtactcg
     4741 atagtgcatc ttcagaaaat ccaatactaa tttcacatgc ttctggtcat atgggagttg
     4801 caaatacatt ggggttaaaa cagcttggtg ttaccaatga aacacgtgac ccagaaggtg
     4861 gacatatagg tagagtagaa ggcagtgaag aaccaaatgg atatttagaa gaaaatgcat
     4921 tttttaatgt agcatctaaa ataaaacaac catcatcaaa tgagattttt aattcaattg
     4981 aaaaagcaca gaacatatat ctaagctatg gtattacaac agctcaagaa ggtttaatgg
     5041 aagaaaatca atttaacatt ttaaaagcaa tggcaaatca aaataaatta aaaatggatg
     5101 ttgtaggata tgtaaatttg aaaaaaagta agtctgtagc agataataat agggagttta
     5161 taaaaaaata tataaataga tttaaaatag gaggttataa gattttctta gatggttctc
     5221 cacaaggtaa gacagcatgg ttgagtagac catatgaaaa ttcagatgat ggttattgtg
     5281 gatatcctat atacaaagat gaagaagtag aaaagtttat agatatttca ctaaaagaaa
     5341 agatgcaact cttaactcat tgtaatggag atgcagcagc agaccagttg atagatgcgt
     5401 ttgagaaagt tttaaattta aaagagcaaa gtagtgaaaa tcatataaga ccagttatga
     5461 tacatgctca aacagtaaga gaagaccaga ttgatgatat gaaagtaata aatatgatac
     5521 cttcatactt tgtagctcac acatattatt ggggtgatat tcatatcaaa aaccttggag
     5581 aagatagagc gtttaagatt agcccactta aaacaactat agaaaaggga ttaatttaca
     5641 ctcttcatca ggatacacca gtaatagcac ctaatatgct ggaaacagta tggtgtgctg
     5701 tcaatagaat tacaaaaaaa ggtatacaaa taggagaaaa tgaaaagata agcccattag
     5761 atgctttaaa gggtgttact ataaatgcag catatcaata ttttgaagaa gataaaaaag
     5821 gttcaattaa agaagggaaa ttagctaatt tgataatctt agatgaaaac ccacttacaa
     5881 tcgaccctat gaaaataaaa gatattaaag tattgcagac tataagagaa ggagaagtat
     5941 tgtattcttt gaagtagatg aaagttagat attttataat ctataaaaaa tagttttata
     6001 taataagtta ggtagaatt
//
LOCUS       DAMJYR010000191         5983 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.196, whole genome shotgun sequence.
ACCESSION   DAMJYR010000191 DAMJYR010000000
VERSION     DAMJYR010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5983)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5983)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5983
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.196"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            157..1290
                     /locus_tag="PC471_003554"
     CDS             157..1290
                     /locus_tag="PC471_003554"
                     /inference="COORDINATES: protein motif:HMM:NF025330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kelch repeat-containing protein"
                     /protein_id="HDF2790637.1"
                     /translation="MTIKLEEDSSIKEVMDSFENIKNDLQNAKDNLASTLGSPFVSTD
                     SFSTTKTKIQTFKSTLATNLTKKGVSTSSNETVQNMVSKVDDIELGNTDIPNWYKPKN
                     IIFDGVTSALQQYPPCVAVENYIYFLCNNSLGLSLLRYDTLTNVNSVLAKPSVCDDAL
                     MVYYDGYIYRIGGYSNSSANSTCQKYNIKTNTWSYISNMITPRGGTCGNTYSNEIHVL
                     YGYTDTNSNSTASAISEYYKISTNTWSTKTSFPPSRDRRLFVCAKTGTYYFCITGATD
                     PNNIWETYINRYDAGNGALTKIEGWYKSFATSINDMLYTVYYGDTYLYVKGYNQNGRV
                     FSKEYVKKNSTFPYNPLLKAVSVKNKYIYYYENNMYKCFIPEL"
     gene            1308..1673
                     /locus_tag="PC471_003555"
     CDS             1308..1673
                     /locus_tag="PC471_003555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2790638.1"
                     /translation="MIKIWDKKEKINGVSAEEILKGNYDFQTSEVFLILDDYNRVTNI
                     ESVNTIKSIYKLDKNLTALETAEKYLEMHKKQEEDIKAHEELEKNSKNATIATHEEVK
                     ALREETATLTFAMMEKELI"
     gene            1674..1808
                     /locus_tag="PC471_003556"
     CDS             1674..1808
                     /locus_tag="PC471_003556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016034205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HDF2790639.1"
                     /translation="MWYKIIKKYYEDELWTKEQVKTAVEKNKITTIEYKNIVGEDYIV
                     "
     gene            2019..2624
                     /locus_tag="PC471_003557"
     CDS             2019..2624
                     /locus_tag="PC471_003557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2790640.1"
                     /translation="MIEKLNENASLSDLITTFENVKDELQIGKNNIASTLGNPFVGTD
                     KLSVTKTKIQTLKNTLAISLSMKNISAQSTETLHSLIGKVAGIANIFIAQGECTQVGE
                     KTFQLYQVDNNTPKTCSANAISFNLAFTPKFIIVYRNAYNIGLCSFVFYSSFRNIITA
                     VPQPSNFGEMYMPNTFDCVVGHTCILPVKDYKIPHFFYAIA"
     gene            2648..2953
                     /locus_tag="PC471_003558"
     CDS             2648..2953
                     /locus_tag="PC471_003558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2790641.1"
                     /translation="MTEYVEVGRRIFFDEEGKIIFYEGQSKGNVPDRKNIKKIEYIDL
                     EYDYVDYDKYKIIGIDIRTKQPILEEIPVYMSEEGKRIQELENQILIAENEKSGGLL"
     gene            2953..3135
                     /locus_tag="PC471_003559"
     CDS             2953..3135
                     /locus_tag="PC471_003559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2790642.1"
                     /translation="MNINNVVVRILAERILNGGLNPLKNREFQLDDVTNTEYRKAIED
                     YIIKNSGVVEGAEPTI"
     gene            3169..3735
                     /locus_tag="PC471_003560"
     CDS             3169..3735
                     /locus_tag="PC471_003560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2790643.1"
                     /translation="MTFKELVNKVRNLVLEAKKVTIEDTENKFTSENVEEALKECINR
                     ADEAFQEADSGKELIATAIGSPATSEQTFQDYANYITGFKSNISNLETQLKSKYSIRY
                     GPIDGYDGNPFSANFGKSASYLIVYVYFRRSVYYYNPSGSSLGSNTGGSERAWITIDS
                     NKTGFSVHSYDTSYEPYPFTGYYIACFA"
     gene            3766..4056
                     /locus_tag="PC471_003561"
     CDS             3766..4056
                     /locus_tag="PC471_003561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2790644.1"
                     /translation="MEEISINLLCAVAGVVISYLAFRNSSNRKIQDDTETTTKLEQQI
                     TFLCENVRDIKHDVAKFNTSFLDISERVAKVEASTKQAHLRIDEIINRIGGK"
     gene            4060..4317
                     /locus_tag="PC471_003562"
     CDS             4060..4317
                     /locus_tag="PC471_003562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HDF2790645.1"
                     /translation="MDNLISFIPEQLLILVAALYVLGVGFKKYKQLDNKYIPVVLLIL
                     GIGFSIWMLGLNPVAVLQGVICWGVAIGINQTYKQLKDGEK"
     gene            4578..5441
                     /locus_tag="PC471_003563"
     CDS             4578..5441
                     /locus_tag="PC471_003563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Abi family protein"
                     /protein_id="HDF2790646.1"
                     /translation="MVEVKEEKTFDEQIDILKSRGLIINDKEDAKFVLSNVNYYRFTA
                     YLLSFKNDDGSYKEGTTFEEVYDIYRFNKEFRILLTDLLGSIEIAFRTYIAYTLAIKH
                     GACGYLERESFKDEKFYINFLTALEREKSNNSDKLFIIHHKEKYEGKLPIWVATEIMT
                     FGMLSKLYSNMLPEDTRYIKNNLCRVNTLLVKSWLQSLTQVRNQCAHYGRIYNNNFRI
                     ITIKNEYKKYNLDNKKIFSYILAMKHLTMDKLIWNSFFIKLQKLINDYNNSIDLKLIG
                     FPNNWIEILAK"
     gene            5520..>5983
                     /locus_tag="PC471_003564"
     CDS             5520..>5983
                     /locus_tag="PC471_003564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HDF2790647.1"
                     /translation="MKICITVGHSILKSGACTSADGVVNEYQYNKSLAPVLADTFRKE
                     GHKADVIICPEKQFKTKAEEKTYKIPRVNSGGYDLLIELHLNASDGQGKGSEVLYYSN
                     KGLEYATRICNKLGTVFRNRRAKLDKGLYILNSSNPTAVLIESFFCDNKEDY"
BASE COUNT         2453 a          661 c         1004 g         1865 t
ORIGIN      
        1 acttttgtaa tctttgaatt tactacattg tatattaaag taaattaatt taaaatataa
       61 agctgttttt agaatagttt aaatttctgt cccttttact tttttaagta accatttcta
      121 aacgaaagga tacttaaaaa aggaggcagg aatttaatga ctataaaatt agaagaagat
      181 tcaagtataa aagaagttat ggactctttt gagaatataa aaaatgacct tcaaaatgct
      241 aaagacaact tagctagtac attaggaagt ccttttgttt caactgattc atttagtact
      301 actaaaacaa aaatacaaac tttcaagagt acattagcaa ctaatttaac taaaaaaggt
      361 gtttcaactt cttcaaatga aaccgtccaa aatatggtaa gtaaagttga tgatattgaa
      421 ctaggaaata cagacatacc aaactggtat aaacctaaaa atataatttt tgatggagtt
      481 acttcggcat tacaacaata tcctccatgt gtagcagtag aaaactatat atattttctt
      541 tgcaataaca gtttaggttt atcgctttta cgctatgata cattgacaaa tgttaatagt
      601 gtcttagcga aaccatcggt ttgtgatgac gctttaatgg tttattatga tggttatatt
      661 tacaggatag gtggatatag caatagttcg gcaaattcaa cttgccaaaa atataatata
      721 aaaacaaata cttggtcgta tatttctaat atgattacac ctagaggtgg aacatgcggg
      781 aatacttatt ctaatgaaat acatgttttg tatggttaca cagatacaaa tagtaattca
      841 acagcaagtg ctatcagtga atattataaa attagtacaa atacctggtc aactaaaaca
      901 agctttccgc catctcgaga tagaagatta tttgtatgtg caaaaacagg cacctattat
      961 ttttgtataa caggagcaac agacccaaat aatatttggg aaacttatat aaatagatat
     1021 gatgcaggaa atggagctct tacaaaaata gaaggttggt ataaaagttt tgcaacaagt
     1081 attaatgaca tgttatatac tgtgtattat ggcgatacat atctttatgt aaaaggatac
     1141 aatcaaaatg gaagagtatt ttctaaagaa tatgttaaaa aaaattctac atttccatac
     1201 aatccactac ttaaagcagt ttcagtgaag aataaatata tctattacta tgagaataat
     1261 atgtataaat gctttatacc tgaattataa aaaggagaaa ataaaaaatg ataaagatat
     1321 gggataaaaa agaaaaaata aatggagttt cagcagaaga aattttaaaa ggtaattatg
     1381 attttcaaac aagtgaagta tttttaatac ttgatgatta taacagagtt acaaatatag
     1441 agtcagttaa tacaattaaa tctatatata aactagataa gaatttgact gctttagaaa
     1501 cagcagaaaa atatttagaa atgcataaga aacaagaaga agatataaaa gcacatgagg
     1561 aactagaaaa aaattctaaa aatgcaacta tagctacaca cgaagaagta aaagcattaa
     1621 gagaagaaac agcaacatta acttttgcaa tgatggaaaa ggagttgata tagatgtggt
     1681 ataaaataat aaaaaaatac tatgaggatg aattatggac taaagagcaa gttaaaacag
     1741 cagtagaaaa gaataagata acaacaatag aatataaaaa tatcgttgga gaggattaca
     1801 ttgtatagtc cttttttaat tttatatagt aactatttgt caagaaagag ttactaaaaa
     1861 gctttatact tgtttatttt gcttgttttt ctattaaaaa taaaattatt tatactatat
     1921 atactttgaa aaaattactt aaaatcaatc acttttctat tttaaaaagt aaccatttct
     1981 tgacaaacag ttactaaaaa tagaaaggtg atatttttat gatagaaaaa ttaaacgaaa
     2041 atgctagtct aagtgactta ataacaactt ttgagaacgt gaaagatgaa ttgcaaattg
     2101 gtaaaaataa tatagctagt acattaggaa atccttttgt tggaactgat aagctaagtg
     2161 taactaaaac caaaatacag accttgaaaa atacactagc aattagtttg tctatgaaaa
     2221 atatatctgc tcaatcaact gaaacacttc attcactaat tggtaaagta gctggaatcg
     2281 caaatatttt tattgcacaa ggagaatgta ctcaagttgg agaaaaaact tttcaactct
     2341 atcaagtaga taataatacg ccaaaaactt gttcagcaaa cgcaataagt tttaacttag
     2401 cttttactcc aaaatttatt attgtctata gaaatgctta caatataggt ttgtgtagtt
     2461 ttgtttttta ctcatctttt cgtaatatta taacagcagt gcctcaacct agcaattttg
     2521 gtgaaatgta catgcctaac acattcgatt gtgtagtagg tcacacttgt attttgccag
     2581 tgaaagatta caaaatacca cattttttct atgcaattgc ttaaaaaata agttaaagga
     2641 ggaaactatg actgaatatg tagaggtagg aagaagaata ttttttgatg aagaaggcaa
     2701 aataatattc tatgaaggac aatcgaaagg aaatgttcca gatagaaaaa atataaaaaa
     2761 aatagaatat atagatttgg aatacgatta tgttgattat gataagtaca aaataatagg
     2821 aatagatata agaactaagc aaccaatctt agaagaaata ccagtatata tgagtgaaga
     2881 aggaaagaga atacaagaat tagaaaatca aatattgatt gcagaaaatg aaaaatcagg
     2941 aggactatta taatgaatat aaataatgtt gtagtaagaa tattagcaga gagaatatta
     3001 aacggagggt taaacccatt gaaaaataga gaatttcagt tagatgacgt gactaacaca
     3061 gagtacagaa aggctataga ggattatatt ataaaaaata gtggagtagt agaaggagca
     3121 gaaccaacta tatagagggt tcttttttta ttgaaagaag gtgactaaat gacttttaaa
     3181 gagttagtta ataaagttag aaatcttgta ttagaagcaa agaaagtaac catagaagat
     3241 acagaaaata agtttacaag tgaaaatgta gaagaagcat tgaaagaatg tataaataga
     3301 gcagatgagg cttttcaaga agccgatagt ggaaaagagt taattgcaac tgctatcggc
     3361 tctcctgcta catcagaaca aacatttcaa gattatgcga actatattac aggatttaag
     3421 agcaatataa gcaatttaga aactcaattg aaaagcaaat attctattag atatggtcct
     3481 attgatggat atgatggtaa tcctttttct gctaattttg gcaagagtgc aagttacctt
     3541 attgtctatg tttactttag aagaagtgta tattattata atcccagtgg tagttcttta
     3601 ggaagtaata caggaggttc tgaacgtgca tggattacta tagacagcaa taaaactggt
     3661 ttttcagttc attcatatga tactagttat gagccatatc cttttacagg ttattatatt
     3721 gcttgtttcg cataataaat tatattatta aaactaggag gatgtatgga agaaattagc
     3781 ataaatctat tatgtgcagt tgcaggagtt gtaatatcct acttagcatt tagaaatagc
     3841 tcaaacagaa agatacaaga tgatacagaa acaactacaa aattagaaca acaaataact
     3901 tttctgtgtg agaatgtaag agatataaag catgatgtag caaagtttaa tacaagtttc
     3961 ttagatatca gtgaacgagt tgcaaaagta gaagcaagta caaaacaagc acatcttaga
     4021 attgatgaaa ttataaatag aattggagga aaataaaaga tggacaattt aataagtttt
     4081 ataccagagc agttgctaat tttagtagct gctctttatg ttttaggtgt tggattcaag
     4141 aaatataaac aattagataa taaatacatt ccagtagtgt tactgatact tggaataggt
     4201 ttctcaatat ggatgctagg attaaatcct gttgcagtct tacaaggtgt aatttgttgg
     4261 ggagttgcaa taggtataaa tcaaacttac aaacagttga aggatggtga aaagtaatga
     4321 aattaacaaa aatattatta ctcacaaaaa tttaaaaaat ttcattaaat agagtatact
     4381 tttatagcta ataagtatat aataatagta cagaactcgt aaagcattta tatgctcaaa
     4441 tatacgggac attgattttt gaaggaaccc gccaagcttc ttttatagct caaataggcg
     4501 ggacagataa atttacccac taatattaat tggtgggttt tattatataa aagtatatct
     4561 atgaaaaggt gaaaacaatg gttgaagtaa aagaagaaaa aacatttgat gaacaaatag
     4621 atattttaaa aagtagagga ttaataataa atgataaaga agatgctaaa tttgtattaa
     4681 gtaatgtcaa ttattatagg tttacagcat atcttctaag ttttaagaat gatgatggct
     4741 catataaaga aggaactacg tttgaagaag tttatgatat atataggttt aataaggaat
     4801 ttaggatatt attaacagat ttgttaggga gcatagaaat agcatttagg acatacattg
     4861 catatacatt agcaattaaa catggtgctt gtggatatct agaaagggag agtttcaaag
     4921 atgaaaaatt ctatattaat tttttgacag cattagagag agaaaaaagt aataattcag
     4981 ataagctttt tattatacac cataaagaaa aatatgaagg aaaacttccc atttgggttg
     5041 caacggaaat aatgactttt ggtatgttgt caaaattata ttcaaacatg ttgccagaag
     5101 atactagata cataaaaaat aatttgtgta gagtgaatac tttattagtt aaatcttggt
     5161 tacaatcatt aacacaggtc agaaatcaat gcgctcatta tggtagaata tataataata
     5221 atttccgtat tataacaata aaaaacgaat ataaaaagta taacttggat aataaaaaga
     5281 tattttctta tatacttgct atgaagcatt tgactatgga taaattaatt tggaatagtt
     5341 tttttataaa acttcaaaag ttaattaatg attataataa ttctatagac ttaaagctta
     5401 ttggttttcc taataattgg atagagatat tggctaaata aaatagttac tttaagaagt
     5461 ttataaacac ttactatatg taagtgtttt tttattgaaa agaaggagga aaataaataa
     5521 tgaaaatatg tattacagta ggacacagta ttttaaaaag tggtgcatgc acttctgctg
     5581 atggagtagt taacgaatac caatacaata aatctcttgc accagtatta gcagatacat
     5641 ttagaaaaga gggtcataag gcagatgtaa taatatgccc tgaaaagcag tttaaaacta
     5701 aagcagaaga aaagacttat aaaataccta gagttaatag tggaggatat gatttactta
     5761 tagaactaca tctaaatgca agtgatggtc aaggaaaagg ttcagaagtt ctatattata
     5821 gtaataaagg tttagaatat gcaactagaa tatgtaataa gctaggtaca gtatttagaa
     5881 atagaagagc taaattagat aaaggattat atatcttaaa tagttcaaat cctacagcag
     5941 tattaattga aagtttcttc tgtgataata aagaagatta tga
//
LOCUS       DAMJYR010000192         5934 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.197, whole genome shotgun sequence.
ACCESSION   DAMJYR010000192 DAMJYR010000000
VERSION     DAMJYR010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5934)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5934)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5934
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.197"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            103..669
                     /locus_tag="PC471_003565"
     CDS             103..669
                     /locus_tag="PC471_003565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HDF2790648.1"
                     /translation="MSVSLQKGQRVSLVKDNNPVKNLVVGLGWDMNKLGKKNYDLDAF
                     AIALTNQDKMNVRGDLVYFGNLKHPSKAIIHTGDNLTGKGEGDDEQIIVNLEDIPEYV
                     HKIVFGVSIYKAKKRDQDFGQINNSFIRLIDSNSKQELFKYNLQEDFSGKVTVLAGEI
                     YRRNEEWKFNALGIGQNEELRELINTYK"
     gene            709..1290
                     /locus_tag="PC471_003566"
     CDS             709..1290
                     /locus_tag="PC471_003566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HDF2790649.1"
                     /translation="MGITLAKGQKVSLTKSNPGLKKVIVGLGWDINKYDGGFDFDLDA
                     SAFLTGTDGKVTNDGDFIFYNNLKHASGAVEYMGDNRTGVGDGDDEQINVDLSKIPQN
                     IAKISFSVTINEAIIRRQNFGQVENSYIRIYNEETNEELIKYELGEDFSIETAIVVAE
                     LYRHNGEWKFNALGSGFEDGLAGLCKNFGVNIG"
     gene            complement(1347..2264)
                     /locus_tag="PC471_003567"
     CDS             complement(1347..2264)
                     /locus_tag="PC471_003567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDF2790650.1"
                     /translation="MTIRSLEIFVKVAECGKMSEVARNMYITQSSVSQAISEIEKEYG
                     VKLFDRISKKLYLTEAGKKLLGYGRHLLAVNEEMNDCMKHCAKNIRIRVGATVTVGTC
                     VISPIMLELYKVNPLIEPEVFVEDTRLIAKKLLNSELDIAIVEGKIKHPDIVTKSIIN
                     DNLVLICSHKHEFYKRDSIKVSELSNQPLIMRELGSGTRAQLEKQLKELKIPMNIRWS
                     CYNSEAILRAVVDNFGIAVISELLIEDYLKKHLLWACDIEGINLHRTFDIAYHRSKFF
                     TENISAFFDISVEYGKKQARKKSRSVNSL"
     gene            2394..4025
                     /locus_tag="PC471_003568"
     CDS             2394..4025
                     /locus_tag="PC471_003568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HDF2790651.1"
                     /translation="MKVLIIGGVAAGTKTAAKIKREMGDNCSVTILNKGDDISYAGCG
                     LPYYVGKIIEDKSSLIVNTPESFSKLTGAEVRCGVEVTSIDRNAKQVTLKTLSTGEKE
                     NLSYDKLVIATGADPIKPPIPGIDLPGVFFMRTPNDAIKLRDSIENGIKRAVVIGGGF
                     IGLEIAENLSAMGIRVSVVDMAEHVMPGFDTDFAEYVENHMADKGIMIFTGDQVVGIE
                     GENKVEKLRTKNRAIKTDLVVMSVGIRPNTGFLKDTGLEFAPNNTIIADEYMLTNDKD
                     IYVVGDCAFVKNSLTNKPTWSPMGSSANHEGRICAQNISGKSKTYNGVLGTTVVKLPE
                     LNAGKTGLSKEIAEKEGYNVCSVTIATDDKAHYYPGASNFIIRMVAEKNTRKLLGVQV
                     MGPGAVDKIVDIAATAITLGADLYSLESMDLAYAPPFSTAIHPFVVAVNVLQNKLNGE
                     LDSVLLDEIEDFDSWTKLDVSKAPSIPSLRYIPVGEINGEIEGLAKDEKILLICAKGK
                     NSYMAQNRLRRFGYTNTKVLEGGILFYPNLKVMEE"
     gene            4028..4975
                     /locus_tag="PC471_003569"
     CDS             4028..4975
                     /locus_tag="PC471_003569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013378669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4Fe-4S)-binding protein"
                     /protein_id="HDF2790652.1"
                     /translation="MSNVTAEQKKEVKGRGFLPSRDGEHFAARIITVNGVINASQAKK
                     IAEAAEKFGNGQLAFTTRLTVELPGVKFEDIEALSEFIASENLITGGTGSRVRPVVAC
                     KGTVCVHGLANTQALAAEIHEEFYKNWYDVKLPHKFKIGVGGCPNNCIKPDLNDLGIV
                     GQRVPDYDSELCVGCKKCAVVEVCPVKAAKLTDKGKLEIDSNLCNNCGKCIESCNFDS
                     IEEKESGYKVYIGGKWGKSVRPGTQIDRLFSKEELMTLIEKAILLFREQGNTGERFGI
                     TIDRIGADKFIEMLLSDEVLERKEEILEALLHLTGGAVC"
     gene            complement(5260..5523)
                     /locus_tag="PC471_003570"
     CDS             complement(5260..5523)
                     /locus_tag="PC471_003570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1883 domain-containing protein"
                     /protein_id="HDF2790653.1"
                     /translation="MEYSYSKMNLKKGDIVEVNLEKQANVILLDHINYVKFKNQRNYD
                     YYGGFAKKNPCRMRVPNTGTWYLVVNQDGNSGIVNFSINTIQN"
     gene            complement(5622..5867)
                     /locus_tag="PC471_003571"
     CDS             complement(5622..5867)
                     /locus_tag="PC471_003571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2790654.1"
                     /translation="MFIDEELEGYILTCKISEDFKNIPEYSDEEFYVTVYKDESSDSG
                     YYALLENKEERVVWDGEVVANNIFNNLWIVVNKVKTG"
BASE COUNT         2137 a          810 c          977 g         2010 t
ORIGIN      
        1 aaatatagcc aacttagcct aaatacgtat ataatatagc taatatggtt cttatacttg
       61 ataccatcaa taaaactaaa aatataaaaa ggagaactta aaatgagtgt tagtttacaa
      121 aaaggacaaa gagttagctt agtaaaagat aataatcctg taaaaaatct agtagtaggc
      181 ttaggatggg atatgaataa attgggtaaa aaaaattatg acctagatgc atttgcaata
      241 gcattgacta accaagataa aatgaatgtt agaggtgact tggtttattt tggaaatctt
      301 aaacaccctt ctaaagctat tatacacact ggagataact taactggaaa aggtgaaggt
      361 gatgatgaac aaataattgt taatttagaa gacatacctg aatatgtaca taaaatagtt
      421 tttggagttt ctatatacaa agctaaaaaa agagaccagg attttggtca aataaacaac
      481 tcttttataa gattaattga ttcaaattcc aagcaagaat tatttaaata taatcttcaa
      541 gaagactttt ctggtaaagt aacagttttg gcaggagaaa tttatcgtag aaatgaagaa
      601 tggaaattta atgctttagg aattggtcaa aatgaagagc ttcgtgaatt aattaataca
      661 tataagtaat aattacaata aaaaatttat aatgaggagt tggtttaaat gggtataact
      721 ttagcaaaag gacaaaaagt aagtttaaca aaaagtaacc ctggtctaaa aaaagtaata
      781 gtaggactag gatgggatat taataaatat gatggtggct ttgatttcga tttagatgcc
      841 tctgcatttt taactggaac tgatggaaaa gtaacgaatg atggagactt tatattctat
      901 aataatttaa aacacgcttc tggagctgtt gaatatatgg gtgataatag aacaggtgtt
      961 ggtgatggtg atgatgaaca aataaatgta gacttatcta aaatacctca aaatattgct
     1021 aaaatatcat ttagtgttac tataaatgaa gcaattataa gaagacaaaa tttcggacaa
     1081 gttgaaaatt catatataag aatatataat gaagaaacta atgaggaact tattaaatat
     1141 gaattgggtg aagattttag catagaaaca gcaatagttg tggcagaact atatagacat
     1201 aatggagagt ggaaatttaa tgctttaggt tctggatttg aggatggact tgcaggattg
     1261 tgtaaaaatt ttggcgttaa tataggctaa tatatctaaa taaaaataag agtatattga
     1321 tatactctta tttttattta gatatattat aaactgttta cacttctact cttctttctc
     1381 gcttgttttt tcccatattc tactgaaata tcaaaaaatg ctgaaatatt ttctgtaaaa
     1441 aacttgcttc tatgatatgc aatatcaaat gtcctatgta aattaatacc ttcaatatca
     1501 caagcccaca aaagatgctt ttttaaataa tcctcaataa gtaattcgga tataactgca
     1561 atgccaaaat tatctacaac tgctcttaat atagcttcag agttataaca tgaccatcta
     1621 atattcattg ggatttttaa ttcttttaat tgcttttcta attgtgctct tgtaccactt
     1681 cccaattcac gcataattaa tggttgattt gaaagttctg aaacttttat agagtctctt
     1741 ttataaaact catgcttgtg tgaacatata agtacaagat tatcattaat aatgcttttt
     1801 gtaacaatat ctgggtgctt aatttttcct tctacaatcg caatatctaa ttcactatta
     1861 agtaattttt ttgcaatcag tcttgtatct tctacaaata cttcaggttc tataagagga
     1921 tttaccttgt ataattctaa cattattgga cttattacac atgtaccaac agttactgtt
     1981 gccccaactc ttatacggat attttttgca caatgcttca tacaatcatt catttcttca
     2041 tttacagcta aaagatgcct cccatatcca agaagttttt tccctgcctc agttaaatat
     2101 aatttttttg aaattctatc aaatagctta acaccatact ctttttctat ttctgaaata
     2161 gcttggctaa ctgatgactg ggtaatatac atatttcttg ctacttcact cattttacca
     2221 cattcagcaa ccttaacaaa gatttcaaga ctacgaattg tcatattatc acaaccttag
     2281 caaacattag taaatgctta tgattttatt aatattttag tattttacaa atatattgtc
     2341 aagaagtata attttattat agagttataa agtagattgg agtgtgatat attgtgaaag
     2401 tattaattat aggtggagtt gctgctggaa caaaaactgc tgcaaaaata aaacgtgaaa
     2461 tgggtgataa ttgctctgtt acaatactaa ataaaggtga tgatatttct tatgcaggtt
     2521 gtggtcttcc atattacgtt ggtaagataa ttgaagataa atcatcttta atcgtaaata
     2581 cacctgaaag tttttctaaa ctaacaggag cagaagttcg ttgtggagtt gaagttacat
     2641 ctattgatag aaatgcaaaa caagtaactt tgaaaacgct ttcaacagga gaaaaagaaa
     2701 atctaagcta tgacaaactt gtaattgcta caggggcaga cccaataaaa ccaccaatac
     2761 ctggaataga tttacctgga gtattcttta tgcgtactcc aaatgatgct ataaaactaa
     2821 gagattctat tgaaaacggt attaagagag ctgttgttat tggtggaggt ttcattggtc
     2881 ttgaaattgc tgaaaatctt tctgcaatgg gtatacgtgt aagtgttgtt gatatggctg
     2941 aacatgttat gcctggattt gatactgatt ttgctgaata cgttgaaaat catatggctg
     3001 ataaaggcat aatgattttt acaggtgacc aggttgtagg tattgaaggt gaaaataaag
     3061 ttgaaaaact tcgtactaaa aatagagcta ttaaaacaga tttagtggtt atgtcagttg
     3121 gaatccgtcc taacacaggt tttctaaaag acacaggttt agaatttgcc cctaataata
     3181 caattattgc agatgaatat atgcttacaa atgacaaaga tatatatgta gttggagact
     3241 gtgcttttgt aaaaaattct ttaacaaata aacctacatg gtcacctatg gggtcatctg
     3301 caaatcatga gggtagaatt tgcgcacaaa atatatctgg aaaatctaaa acttacaatg
     3361 gtgttcttgg aacaacagta gtaaaattac ctgagctcaa tgcaggaaaa acaggtctaa
     3421 gtaaagaaat agctgaaaaa gaaggttata atgtttgctc tgtaaccata gctactgatg
     3481 ataaagctca ttattatcca ggtgcatcta attttattat aagaatggtt gctgaaaaaa
     3541 atactagaaa attattaggt gttcaagtta tgggacctgg agcggttgat aaaatagtag
     3601 atatcgctgc tactgcaata actttaggcg ctgaccttta ttcattagaa agtatggacc
     3661 ttgcatatgc accacctttt tcaacagcta ttcacccttt tgtagtggca gtaaatgttt
     3721 tacaaaataa acttaatgga gaacttgata gtgttttact tgacgaaatt gaggattttg
     3781 acagttggac taaacttgat gtttcaaaag ctccttctat tcctagttta cgttatattc
     3841 ctgttggtga aattaatgga gaaattgaag gtcttgctaa ggatgaaaaa attcttctta
     3901 tatgtgctaa aggtaaaaat agctatatgg cgcaaaatcg cttaagacgc tttggctata
     3961 caaatacgaa agttcttgaa ggtggaatac tattttatcc aaatcttaag gtaatggagg
     4021 agtaaatatg agtaatgtta cagcagaaca aaaaaaggaa gtaaaaggta gaggttttct
     4081 accaagcagg gatggagaac attttgcagc acgtataata actgtaaatg gtgtaataaa
     4141 tgcatcacaa gcaaaaaaaa ttgcagaagc tgctgaaaaa ttcggaaacg gtcagttagc
     4201 atttactact agactaacag tagaattacc tggtgtaaaa tttgaagata ttgaagctct
     4261 aagtgaattt attgctagcg aaaatctaat aacaggtgga actggatcaa gggttcgtcc
     4321 tgttgtggct tgtaaaggta ctgtttgtgt tcatggtcta gctaatacac aggcacttgc
     4381 tgctgaaata catgaagaat tttacaaaaa ttggtatgat gtaaaactac ctcataagtt
     4441 caaaattggt gttggtggat gccctaataa ctgtattaaa cctgacctaa acgatcttgg
     4501 aattgttggt caacgtgtac ctgactatga ttcagagctt tgtgttggtt gtaaaaaatg
     4561 tgctgttgta gaggtctgtc ctgttaaagc agctaaatta acagataaag gtaaacttga
     4621 aatagatagt aatctatgta ataactgtgg taagtgtata gaaagttgta attttgatag
     4681 tattgaagaa aaagaatctg gttataaagt ctatatagga gggaaatggg gaaaatcagt
     4741 tcgtcctgga acacagattg atagattatt ttcaaaagag gaacttatga ctttaattga
     4801 aaaagcaatt cttttattta gagaacaggg taacacaggt gaacgttttg gaataactat
     4861 cgatagaatc ggtgctgata aatttataga aatgctttta tcagatgaag ttttggaaag
     4921 aaaagaagag atactagagg ctcttttaca ccttacaggt ggagctgtat gctaacaata
     4981 aatattttaa ttgtacagat aacctatgga aaaaatttta gttaagaata attaaaattc
     5041 tatatatgat atatgataaa tgaggctatc tcaaaacaca gatacgcctc tttttcattg
     5101 ttgaaatgat aaagtatatt tgagttcata taaaaaagag tgtctcatga attataaaat
     5161 tcattttgag gcacccacat tattttataa taattgctta ttaattttat tttaactata
     5221 taaaatgtac aaaaattaag aatatccaat attctaaaat taattttgaa ttgtattgat
     5281 tgaaaagttt actattccac tatttccatc ttgatttact actagatacc atgttccagt
     5341 attaggaact ctcattctac atggattttt ctttgcaaat ccaccatagt aatcataatt
     5401 tctttgattt ttaaatttca cataatttat atggtctaac aatattacat tggcttgttt
     5461 ctctaaattt acctctacaa tatccccttt ttttaagttc atcttactat aactatattc
     5521 cattgaatta ctcctccagt atcgaataat ctttgttatt tactcatctc aaatatgtaa
     5581 aaattcattt aaaatattac aaatactata taattagact attatccagt tttaacctta
     5641 tttactacaa tccaaaggtt attaaaaata ttattggcaa caacttctcc atcccataca
     5701 actctttctt ctttattttc taataaagca tagtacccag agtcagaact ttcatcttta
     5761 tagactgtaa cataaaactc ttcatcacta tattcaggta tatttttaaa gtcttcagat
     5821 attttacatg ttaatatata accttctaat tcttcatcta taaacaaagc aattcctcca
     5881 atctctatat ttttactaat tataccatat tatgtcataa actttttatt ttat
//
LOCUS       DAMJYR010000193         5828 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.198, whole genome shotgun sequence.
ACCESSION   DAMJYR010000193 DAMJYR010000000
VERSION     DAMJYR010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5828)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5828)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5828
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.198"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            83..1195
                     /locus_tag="PC471_003572"
     CDS             83..1195
                     /locus_tag="PC471_003572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDF2790655.1"
                     /translation="MNAFIRKRNKNYVVYLEFEDEKTGKRKQKNMGTFDKKREASKRL
                     NEVKESLYNDGFLVPSEITVSEFLLDFLNKYSENISLATYNNYVSICKNYINPSIGKY
                     KIQDLHPVHIQNYIDKLSYKLNPQSIKIHINILKLAIKRAYRLKLIKENVMDSIEAPR
                     YKKFKNEIYDREQMIKLLDLAKNTEMELPINLAIGLGLRISEVLGLTWDNIDFEENTI
                     TVNKITSRINGSVILKEPKTESSVRKISSPKELMSLLKEYKIKQNKNLLKSSIRNNNN
                     LLFFNKKCEPIAEDVMSKKFKRFLEKNELPHIRFHDLRHSHVTLLINSKVPIKVISER
                     VGHSNISTTLSVYSHVLKEMDKEASDKISESLFNAN"
     gene            complement(1403..1879)
                     /locus_tag="PC471_003573"
     CDS             complement(1403..1879)
                     /locus_tag="PC471_003573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2790656.1"
                     /translation="MVKKRLVAVVISLAMISVGNFSYAHSGGHNSSGGHHNKNKSELT
                     RYYCGGHSAHQHNKGFCPYASTKENSGVTISSVHNEKNHKNYFAEKGYNKGYEDGCKG
                     LYNLSVTEAKSDNLYEKEILKSSYEKGYLIGYNEYKAKKVDDVMKKMLDIPMLASI"
     gene            complement(2078..3703)
                     /locus_tag="PC471_003574"
     CDS             complement(2078..3703)
                     /locus_tag="PC471_003574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDF2790657.1"
                     /translation="MLQVTGVGLRFGDKELFKDVNLKFTKGNCYGIIGANGAGKSTFL
                     KILSGEIEPNTGSISITDKERMSVLKQDHFEYEEETVLNVVIRGHERLWNIMKEKDAL
                     YMKEDFNEEDGIKAAELEGEFAELDGWDAETNAEKILMGLGITKDMHYKQMKELVGGE
                     KVKVLLAQSLFGKPEILLMDEPTNHLDFKSINWLNNFIMDLEESIVIIVSHDRHFLNQ
                     ICTNIVDVDFGKIQMYVGNYDFWYESSQLALQLAKDQNKKTEEKIAQLKEFIARFSSN
                     ASKAKQATSRKKQLEKLEVEDIQPSRRRYPYVGFTPAREIGNEVLEVHNLTKTIDGVK
                     VLDNVSFRLDRDDKVVFMGDEIATTALLNIVMGELEPDSGEYKWGVTTSQDYLPKNHN
                     KFFDGVEYSLVDWLRQFSEEKSESFIRGFLGRMLFSGEEALKEAQVISGGEKVRCMLS
                     KLMLSNANVLVLDDPTNHLDLESITSVNKGLEKFPGVLLFTSHDHEFISTIANRIIEI
                     TPNGIMDRKMDFDEYLESKEIQDQLAKMYGKDK"
     gene            complement(4041..5060)
                     /locus_tag="PC471_003575"
     CDS             complement(4041..5060)
                     /locus_tag="PC471_003575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HDF2790658.1"
                     /translation="MKLIKTIDAVGQVLCHDITQIIPGEFKGRKFKKGHIVKEEDIPV
                     LLSLGKDNLYVWEKSEGMVHENEGALFLKELTAGENLDFSEIKEGKIDFIAACDGLLK
                     IDVDALFDLNCVGEIMMATLHNNFIVNKGLKVAGTRVIPLVIDEKKLEDAKKVVGNRK
                     IVNVVPFKPKKVGIVTTGNEVFYSRIVDKFGPVIEEKVKGFGCEVIGQTICPDDKEII
                     KSAIKEFISQGAELICCTGGMSVDPDDVTPTAIKETGADLVTYGSPILPGAMFLLAYY
                     GEVPIMGIPGCAMYHKTTVFDIVLSRVLIDEKLDKYDIARYGHGGLCMNCDVCTYPAC
                     NFAKI"
     gene            complement(5057..5626)
                     /gene="mocA"
                     /locus_tag="PC471_003576"
     CDS             complement(5057..5626)
                     /gene="mocA"
                     /locus_tag="PC471_003576"
                     /EC_number="2.7.7.76"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor cytidylyltransferase"
                     /protein_id="HDF2790659.1"
                     /translation="MINAVIMASGFARRMGANKLLLNYKGCSIIEHVFREVSKIDFHQ
                     VVVVSQYEEVLLLCNKYGFEYIDNKNANIGQSESIKLGVLNSSKCDGYMFFVGDQPFI
                     DNLYIDKIINTFKLDRDFIVIPRYKDMCGNPVIFPYSKKEELLALKEDEKGKTILKNS
                     SKIKYVEVPKEMLFDIDTKMDYERIGGKL"
BASE COUNT         1983 a          983 c          592 g         2270 t
ORIGIN      
        1 tgtatgcaac tctatttcaa aattattttg tgatatatat atgttataat aaatgtataa
       61 aattttgatt ggtgggttat atatgaatgc atttattaga aaaagaaata aaaactatgt
      121 tgtttacctg gaatttgaag atgaaaagac aggtaaaaga aaacaaaaaa atatgggtac
      181 ttttgataaa aaaagggaag caagtaaaag actaaatgag gtaaaagaaa gcttatataa
      241 tgatggtttt ttagtaccaa gcgaaattac agtgtcagaa tttctattag attttctaaa
      301 taaatatagt gaaaatatat ctttagctac atataataat tatgttagta tttgtaagaa
      361 ttacattaat ccatctattg gaaaatataa aatacaagac ttacatccgg ttcacataca
      421 aaactacata gataaactat cttataaact aaatcctcaa tcaattaaga ttcatataaa
      481 tatattgaaa cttgcaataa aaagagctta tagactcaaa ttaataaaag aaaatgttat
      541 ggatagtata gaggctccaa gatataaaaa atttaaaaat gagatttatg atagagaaca
      601 aatgataaaa ttattagatt tagcaaaaaa tacagaaatg gaattaccta ttaatttggc
      661 tattggatta ggtcttagaa tttcagaagt tttaggtcta acttgggata atattgattt
      721 tgaagaaaat acaataacag taaataagat aacttcccga attaatggtt ctgttattct
      781 caaagagcca aagacagaaa gctctgttag aaaaatatct tctcccaaag aattaatgtc
      841 tttattaaaa gaatataaaa taaaacagaa taaaaattta ttaaaatctt ccattagaaa
      901 taataataat ttgttatttt tcaataagaa atgtgaacct attgctgaag atgttatgag
      961 taaaaaattt aaaagatttt tagaaaagaa tgaattacct catattagat tccatgattt
     1021 aagacactca catgttactt tacttataaa ttctaaagta cctataaagg ttatatccga
     1081 gagagtagga cactcaaata taagtactac tctaagcgtt tattctcatg tacttaaaga
     1141 aatggataaa gaggcttctg acaaaatttc tgaaagttta tttaatgcta attaatatta
     1201 atttatctta gctttaaatt agcttagctt attgagatag attcattaat attaaatgta
     1261 tctatctcaa attaataact acttaaataa tctgttttat tttaatttat cgcctcagtt
     1321 attatacttt tttacgaaaa attatacttt acaataaaaa agatattccc ttatagaaaa
     1381 tatcttttta aatactatat tattatatac ttgctaacat tggaatatct aacatcttct
     1441 tcattacatc atctactttt tttgctttat attcattgta tccaattaaa tatccttttt
     1501 catatgaact ttttaaaatc tctttttcat ataaattatc agatttggct tctgtgacac
     1561 ttaaattata aagcccttta cacccatctt cataaccttt attataccct ttttctgcaa
     1621 aatagttttt atgatttttt tcgttatgta ctgaagatat tgttactcca gaattttctt
     1681 ttgtactagc ataaggacaa aaacctttat tgtgttgatg tgctgaatgt cctccacaat
     1741 aataacgtgt taactctgat ttatttttat tatgatgacc tcctgatgaa ttatgccctc
     1801 ctgagtgagc atatgaaaaa ttccctacac taatcatagc taaactaatt actacagcta
     1861 ctaacctttt cttaaccata atctattccc cccaaaatgt tatatatttt tattatatca
     1921 tctactatat tatatttaaa ttcttaatat acaaaatatt actctattct gctaataaac
     1981 atgtttaaac aatataaaaa acctcctaat atttgattaa aaatttacac actcatgcat
     2041 agcatgtctc aaatattaaa aggttttata ctaataatta tttatctttt ccatacattt
     2101 tagctaattg gtcttgtatc tctttacttt ctaaatactc atcaaagtcc atttttctat
     2161 ccattattcc atttggagtt atctctataa ttctatttgc tatagtagat ataaattcat
     2221 ggtcatgaga agtaaataaa agcactcctg ggaacttttc aagtccttta tttacagaag
     2281 ttatactttc taggtctaag tggttagtag ggtcatctaa tacaagtaca tttgcatttg
     2341 aaagcattag cttactaagc atacatctaa ccttttctcc tccagatata acttgagctt
     2401 cttttaatgc ttcttcacct gagaataaca ttctacctaa aaatcctctt ataaaacttt
     2461 cacttttttc ttcagagaac tgtctaagcc agtcaaccaa agaatattct actccgtcaa
     2521 aaaacttatt atgatttttt ggaagataat cttgagaagt agttactccc catttatact
     2581 ctccactgtc tggttcaagt tctcccataa ctatgtttaa aagtgcagta gtagctattt
     2641 catctcccat aaatactact ttatcatctc tatccaatct aaatgacaca ttatcaagaa
     2701 ccttaacccc atctatagtt ttagttaaat tgtgtacttc taaaacttca ttcccaattt
     2761 ctcttgcagg tgtaaatcct acatatggat atcttcttct tgatggttgt atatcttcaa
     2821 cttctaattt ctctaactgt ttctttcttg atgttgcttg tttagccttt gaagcattag
     2881 aactaaatct agctataaat tcctttaatt gagctatctt ttcttcagtc tttttatttt
     2941 ggtccttagc caattgtaaa gccaattgac ttgactcata ccagaaatca tagtttccaa
     3001 cgtacatttg tattttacca aaatctacgt caactatatt agtacatatt tgatttagga
     3061 aatgtctatc atgtgatact attataacta tagattcttc taaatccatt ataaaattat
     3121 ttaaccagtt tattgattta aaatctaagt ggtttgtagg ttcgtccatt aatagtattt
     3181 caggcttacc aaaaagtgat tgtgctaaca ataccttaac cttttcccca ccaactagtt
     3241 ccttcatttg tttgtaatgc atatctttcg ttatgccaag acccataagt attttctcag
     3301 cattagtttc tgcatcccat ccgtcaagct ctgcaaactc accttcaagt tcggcagctt
     3361 ttatcccatc ttcttcatta aagtcttcct tcatatacaa tgcatctttt tctttcatta
     3421 tgttccaaag tctctcatga cctcttataa caacatttaa aacagtttct tcttcatatt
     3481 caaagtggtc ctgttttaaa actgacattc tttctttatc agttatagat atactacctg
     3541 tattaggctc tatttctcct gataatattt ttaaaaaagt agattttccc gcaccattag
     3601 ctcctattat tccatagcaa ttacctttag tgaattttaa gttaacatct ttaaatagtt
     3661 ccttatcacc aaatctaagt ccaacacctg taacttgtaa cattaatata ttttcctttc
     3721 tttttgctca taatttattt tatcataaaa ctttgataac atttttatta tatattatca
     3781 cgtaaatagt atatttttta tactaaattt gtgaaatatt tattaaaata atactaaaaa
     3841 ttgtaaattt ataaaaatta acaatctctt atattatact tataaagaaa tcaaattgca
     3901 actttattca ttcttttaca cattgaaaat aagttattat aatttctaaa taaatatgct
     3961 ttttttaaaa catcattcta gctgttttgt ttcatattta aaaagagtac ttaatttgta
     4021 aatactcttt tttaataact ttatatttta gcaaaattac atgcaggata agtacataca
     4081 tcacagttca tacaaaggcc accatgacca tatctagcta tatcatactt atcaagcttt
     4141 tcatcaatta aaaccctaga taaaactata tcaaatactg tagttttgtg atacattgcg
     4201 catcctggta tacccataat tggaacttct ccgtaataag caagtaaaaa catagctcct
     4261 ggtaatattg gtgaaccata tgtcactaaa tcagctccag tttcttttat agctgttggc
     4321 gtaacatcat ctgggtctac actcatccca ccagtacaac agataagttc tgctccttga
     4381 cttataaatt cttttattgc actctttata atttctttat catctgggca tatagtctgt
     4441 cctataactt cacaaccaaa gccttttact ttttcttcta ttactggtcc aaatttatca
     4501 acaattctgc tataaaacac ttcatttcct gtagtaacaa ttcctacttt cttaggttta
     4561 aaaggtacta catttactat ttttctatta ccaactactt tttttgcatc ttctaatttt
     4621 ttctcatcta taactaaagg tatcactctt gttccagcta cttttaaacc tttatttact
     4681 ataaaattat tatgaagagt agccatcatt atttctccaa cacaattcaa atcaaaaagt
     4741 gcatcaacat ctatctttaa tagaccatca catgctgcta taaaatctat tttcccttct
     4801 tttatttcac taaaatctaa attttctcca gcagttagtt cttttaaaaa caatgcgcct
     4861 tcattttcat gaaccattcc ttctgacttt tcccatacat acaaattatc tttaccaaga
     4921 gataataaca ctggaatatc ttcttctttt actatgtgac cctttttaaa ttttcttcct
     4981 ttaaattctc caggtattat ttgagttatg tcgtgacata aaacttgtcc aacagcatct
     5041 atagttttta ttagcttcat aatttacccc caattctctc atagtccatt tttgtatcga
     5101 tatcaaataa catttcttta ggaacttcta catactttat ttttgatgaa ttttttaaaa
     5161 tggttttacc tttttcatct tctttaagtg ccaacaattc ttctttcttg ctatatggga
     5221 atatgactgg atttccacac atatctttgt atcttggaat tactataaaa tctctatcta
     5281 atttaaaagt atttataatt ttatctatat ataagttatc aataaatggc tggtctccta
     5341 caaagaacat ataaccatca cattttgagc tatttaaaac tcctaatttt atagattcac
     5401 tttgacctat atttgcattt ttattatcta tatattcaaa accatattta ttacacaata
     5461 acaaaacctc ttcatattga cttactacta caacttgatg aaaatcaatc ttgctaactt
     5521 ctctaaatac atgttctatt atagaacagc ctttataatt taataaaagt ttatttgctc
     5581 ccattcttct ggcaaagcct gatgccatta tcactgcatt tatcatatat atcatccttg
     5641 gtatttataa tatattgata gttctatgat ttttataata tctctattga tattatctat
     5701 atatataata tcatataaac tattttaata cttcaatatc ttacacaaaa tttttgtttt
     5761 acacttccat aaaatatctc tatttcactt tcatattctt taatattttc tattaatttt
     5821 atagctaa
//
LOCUS       DAMJYR010000194         5806 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.199, whole genome shotgun sequence.
ACCESSION   DAMJYR010000194 DAMJYR010000000
VERSION     DAMJYR010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5806)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5806)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5806
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.199"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            333..515
                     /locus_tag="PC471_003577"
     CDS             333..515
                     /locus_tag="PC471_003577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2790660.1"
                     /translation="MFKDKIDECVHIMTAYIASLKEYYSFIETQIGDFIKKYGEDVVE
                     LCLHRVMILLCECGLA"
     gene            1342..2256
                     /locus_tag="PC471_003578"
     CDS             1342..2256
                     /locus_tag="PC471_003578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2935 domain-containing protein"
                     /protein_id="HDF2790661.1"
                     /translation="MIDNQKYVILSLELHLFFSRIMKEHALFLEAGFTNKNYNLAMEA
                     DHYKKQFEDLLSYTVSASNGIIRPDILYSEELVTTLTSVAEQKTEEFTGIEINKNITT
                     RELNLQSGVNPQVGQDLVNYVAQLNSDAIRLLDGLINFKERVLDGVLSCTIFTSNYPL
                     LLEHIIHEANLYRSYVVDLENKIDIESKNAKEIELFWDHIMMEHALFMRGLLDPSEGE
                     LINTSNDFAIKFNELIEKTNEMTDSNIKNITEETLNETVEFKDFKEAGASGIEQCKIK
                     SIILPLLADHVLREANHYIRILESYKNM"
     gene            2646..3293
                     /locus_tag="PC471_003579"
     CDS             2646..3293
                     /locus_tag="PC471_003579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein"
                     /protein_id="HDF2790662.1"
                     /translation="MIRQTPLEKRYDKLGPHIVTALKKRYFDAYYCKTRNEALQQILD
                     LIPQNHVVSWGGSETLKEMGVQTAVREKGFKVIDRDLAKTPEERTEIMRQALLCDTFL
                     MSSNAISEDGQLFNIDGNGNRVAAMIFGPKNVIVVAGMNKIVKNIDDAIQRTRTIASP
                     AVVQRFQDVNTPCYINGSCIDCTSPESICAYMVTTRISRPAKKIKVILVGENLGL"
     gene            complement(3460..4029)
                     /locus_tag="PC471_003580"
     CDS             complement(3460..4029)
                     /locus_tag="PC471_003580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2790663.1"
                     /translation="MKKFILCIIMFAISVLVLAGCTNDNKIKNEVDSMFNSIKTQDAD
                     TVDKYFPKSGLGNLIYEDKEGFKMYSKNMTWEISDIKNNKDKVTVDLKINNTDMVSIL
                     KKNPPKLGEMMPNPTMKDIDSAKKKEFNITLELVKSGDNYVCKVNQESAMKLLNVITG
                     GGIDYLADYNKDYYKQLDEEQKNYENKNS"
     gene            complement(4034..4933)
                     /locus_tag="PC471_003581"
     CDS             complement(4034..4933)
                     /locus_tag="PC471_003581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HDF2790664.1"
                     /translation="MKLLSKVNNNIRLITQVAFTALTNGYVNGFLEGTIYGGESKKLC
                     VPGLNCYSCPGALGACPIGSLQAVLSSREYKFSFYVVGFLMAFGAFFGRFVCGWLCPF
                     GLVQDLLHKVPFPVKIKKVYGDKYLKYLKYIVLIIFVIILPVAIVNVAGGGNPWFCKW
                     ICPSGTLLGGIPLILGNENLRESIGFLFSWKLSILLLILLMSIITYRPFCKYICPLGA
                     IYGFFNPISIYRFKIDKDKCTNCTACQTKCKLDIPIYKDVNSPDCIRCGECIKVCPQN
                     AITTTFSKDKKENNICAKKYRGI"
     gene            complement(4944..5093)
                     /locus_tag="PC471_003582"
     CDS             complement(4944..5093)
                     /locus_tag="PC471_003582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2790665.1"
                     /translation="MSNFIKDNKKGISIIAISIIFIALGTYRGETNTVLTKAIMICLE
                     CIGIG"
     gene            complement(5086..5670)
                     /locus_tag="PC471_003583"
     CDS             complement(5086..5670)
                     /locus_tag="PC471_003583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HDF2790666.1"
                     /translation="MKRFFIVVLGCICVLGMISGCSKKESKPVEKNKNNSEWFSNLDT
                     EDVDGNKVTKEIFSNKELTLINVWTTWCGPCVGEMPELEALSKEYESNNSNVAIKGLV
                     VEVDKTDMRTGLSNEEKNLVKDIMKKSDATYQQLTVSEELKKTDFKRVMEFPTTYFVD
                     KKGNFVGEKVVGANSKEDWKKIIDERLKMVKANE"
BASE COUNT         2136 a          866 c          666 g         2138 t
ORIGIN      
        1 cttagaaaat aatagattac aataactaaa attttaaaat taaattcctt aaaagtatga
       61 ttaattataa ttatataagt atattcttat tacacttata ttaatttata ttaaatatct
      121 actaactcaa atattcataa ttaaacttta aactggataa attttctttt aagaatgaaa
      181 gtttatccag ttttgcgatt ataataacca gttttacata tccacttgta aaatatatct
      241 aagcaatata tcatactata tagatttatg agtgaaataa tatctgctat ttactgaata
      301 tttttatttt tcgtttttta gggggattag ttgtgtttaa agataagatt gatgaatgtg
      361 ttcatataat gactgcatat attgctagtt taaaagaata ctattcattc atagaaactc
      421 aaattggtga ttttattaaa aaatatggag aagatgttgt agagttatgt ctccatagag
      481 tgatgattct actatgcgaa tgtggtttgg cataaaatta aatttattaa aatgggtaag
      541 aagaatccta tataatattc ttcttaccca ttttaatata tcaatttcat agcttatcgt
      601 ttaaaatatt ttacatccat tactagacaa ctttacattc tctttttaat tctagtatat
      661 ttaaatcatt ttagttgctc cttatttatt cttattactt gttttattta taaaataatt
      721 ctttttaata atgttttttg ttgatattcc tatcacacat ccaattattt atcattgtct
      781 tgaaattgtt tgcatactta atttaatcca attgaaacac cccagcatag aatcccctgt
      841 aaaatagaag ttggattaaa tcctaacatc tatacagaaa atactactcc aagcacaagc
      901 aatataatgg aatatattta tcacttaatt ttttatactt tttaaatccc atacctaaaa
      961 cattaagtgc agctattaga attaataatt gttgtggtat aaaattatgc ttgcttcata
     1021 tttattcatt ttttgctata aaagttcttg ttttacaaaa aacatttgtt ttgtatcaaa
     1081 ataatactta taaattacat aattctcata aaaatttcta gtattacttg tactcttaat
     1141 aaataatagc caacaagtac cttaaatcat tatatctata tctaataaga aacacagtaa
     1201 gataatcttt tcgcccataa cttaataaaa actgttgata ttagtagcaa ttgaatttag
     1261 ttaatttatt tataatatac ataaatttaa atattacgaa taaagtatta taaataggat
     1321 actttctaag gggaggaaat tatgatagat aatcaaaaat atgttatttt atcactagaa
     1381 ttacatttat ttttttcaag aattatgaaa gagcatgctc tttttttaga agcaggattc
     1441 acaaataaaa attataatct tgctatggaa gctgaccact ataaaaagca atttgaagat
     1501 ttattatcat acactgttag tgctagtaat ggtataatta gacctgatat attatattca
     1561 gaagagcttg taactactct cacatcagtt gcagaacaaa aaacagaaga gtttacaggg
     1621 atagaaataa acaaaaacat cactacaaga gaattaaatc tacaaagtgg tgtaaaccca
     1681 caagttggtc aagacttagt gaactatgta gctcaactta actctgatgc aataagatta
     1741 cttgatgggc ttattaattt taaagaaaga gtcttagatg gtgtactatc atgtactata
     1801 tttacatcaa actaccctct acttcttgaa catataatac atgaagcaaa tttatatcgt
     1861 tcttatgtag ttgaccttga aaataaaata gatattgagt caaaaaacgc taaagaaata
     1921 gaattattct gggaccatat tatgatggaa catgctctgt ttatgagagg attactagac
     1981 ccctcagaag gtgaactaat aaatacttca aatgattttg ctataaaatt taatgaatta
     2041 attgaaaaaa caaacgaaat gactgattct aatatcaaga acattacaga agaaactcta
     2101 aatgaaactg ttgagtttaa agattttaaa gaagcaggag catcaggaat agaacagtgt
     2161 aagataaaat ctataatatt accactttta gcagaccatg ttttaagaga ggcaaatcat
     2221 tatattagaa tattggagag ttataaaaac atgtaattaa aaattacttt taaaataatc
     2281 tttgcatatt tagataaaat aaaggtacag ttaactgtac ctttattttt tataaactta
     2341 taaaatttaa acaagttatt cataaaataa aataataatc atctataacc cacaacttta
     2401 ttttatatta aataaaaagt attagtggtt atgttttata agtatttctt tattcagtta
     2461 tttattaaaa actaatagta aatcttatac taatcgcata tttatgttat aactatattg
     2521 taactatttt cgtaatctta ggaaaagatt acttataaaa ttaaccttat acaataatat
     2581 atcgtataat cttaattagc ataatagtta caatattaaa atttaaatat gggagtgatt
     2641 ttagtatgat aagacaaaca ccattagaga aacgatatga caagctaggt cctcatattg
     2701 taacagcact caaaaaacgt tattttgatg cttattactg taaaactaga aatgaagctt
     2761 tgcaacaaat tttagattta attcctcaaa atcatgtagt atcatgggga ggatctgaaa
     2821 cacttaaaga aatgggcgta caaactgctg ttagagagaa aggatttaaa gttattgata
     2881 gagatttagc aaaaactcca gaagaaagaa ctgaaattat gcgtcaagca cttctatgcg
     2941 atactttttt gatgagtagc aatgcaatta gtgaagatgg acaactcttt aatattgatg
     3001 gaaatggcaa tcgtgtagct gcaatgatat ttggtccaaa aaatgttata gttgtagctg
     3061 gtatgaataa aattgtaaaa aacattgatg atgcaataca aagaactaga acaattgcat
     3121 ctccagcagt tgttcagcgt tttcaagatg tcaatacacc atgctatatt aatggaagtt
     3181 gtatagattg cacaagtcct gaaagcattt gtgcatacat ggttactaca agaatcagtc
     3241 gtccagcaaa aaaaataaag gtaatattgg taggagaaaa tcttggtcta taaaatatac
     3301 atacatgtaa atagctctat aaataataaa atcatatgaa ataacttatt tctaaaagtg
     3361 gtagaataaa agcttctaca agactttcat tctgccactt ttatattctt ttaatttgtg
     3421 tttctatatt attaattgct ccaattttca tatatattac taactatttt tattttcata
     3481 atttttttgt tcttcatcaa gctgtttata ataatcttta ttatagtcag ctagataatc
     3541 tataccacct cctgtaatca catttaataa tttcattgca gattcttgat taactttaca
     3601 aacataatta tcaccagatt taacaagctc taatgtaata ttaaattctt ttttctttgc
     3661 agaatcaata tctttcatag ttggatttgg catcatttct cctagtttag gaggattctt
     3721 ttttaaaata gaaaccatat cagtattgtt aatttttaaa tctacagtca ctttatcctt
     3781 attatttttt atatctgaaa tttcccaagt catattttta gaatacattt taaatccttc
     3841 tttatcttcg tatataagat tacctaaccc tgattttggg aaatacttat caacagtatc
     3901 tgcatcttgt gtttttattg aattaaacat actatccact tcatttttta tcttattatc
     3961 attagtacaa ccagctaaca ccagtactga tattgcaaac attataatac atagaataaa
     4021 tttcttcatt aaattatatc cccctatact tttttgcaca aatattattc tcttttttat
     4081 cttttgaaaa agttgtagta attgcatttt gtggacaaac ttttatacat tcaccacacc
     4141 taatacagtc tggactattt acatctttat atattggtat atcaagctta cattttgttt
     4201 gacatgctgt acaatttgta catttatctt tatcaatttt aaatctatat attgaaatag
     4261 gattaaaaaa accatagatt gctcctaaag ggcatatata cttgcaaaat ggcctataag
     4321 taatgattga catcaataat atcaataaca aaatacttaa cttccagcta aataaaaatc
     4381 ctattgattc tcttaaattc tcatttccta aaataagtgg tattcctccc aaaagtgttc
     4441 ctgaagggca aatccattta caaaaccaag gatttccacc ccctgctaca tttacaatag
     4501 caactggcaa aataattaca aatattatta agactatata ctttagatat tttagatatt
     4561 tatcaccata aacttttttt atctttactg gaaagggtac tttatgtagt aaatcttgta
     4621 caagtccaaa gggacaaagc caaccacaaa caaatctgcc aaaaaaagca ccaaatgcca
     4681 ttaaaaaacc aacaacataa aatgagaatt tatactctct gcttgataaa actgcttgta
     4741 atgaacctat tggacatgct ccaagtgcac ctggacatga ataacaattc aatccaggta
     4801 cacataattt tttactttct cctccatata tagttccttc taaaaatcca tttacatatc
     4861 catttgttaa tgctgtaaat gcaacttgag taataagtct tatattatta tttactttag
     4921 ataataactt cattcctctt ctcctaacca ataccaatac actcaagaca aatcataatt
     4981 gctttagtta atactgtatt tgtttctcca cgatatgtac ctaatgcaat aaatataata
     5041 ctaattgcaa taatagaaat gcctttttta ttatctttaa taaagttact cattggcttt
     5101 taccattttt agtctttcat ctataatttt tttccaatct tccttactat ttgcacctac
     5161 aaccttctca cccacaaagt ttcctttctt gtctacaaaa taagttgttg gaaactccat
     5221 aactctttta aaatcagttt ttttaagttc ttcagataca gtcaattgtt ggtaagttgc
     5281 atctgacttt ttcataatgt ctttaactaa atttttctct tcatttgaaa gaccagttct
     5341 catatcagtt ttatcaactt ctacaactaa tccctttata gctacattgg aattattact
     5401 ctcatattct tttgataatg cttcaagctc tggcatttca cccacacatg gtccacacca
     5461 agtagtccag acattaataa gagtaagttc tttatttgaa aatatctctt ttgttacctt
     5521 atttccatca acatcctccg tatctaaatt actaaaccat tcactattat tcttgttttt
     5581 ttctactggc ttagattctt ttttgctaca tccagaaatc atacctaaaa cacaaataca
     5641 tcctaaaact actatgaaaa accttttcat atctcacact cctagaattt atatagtata
     5701 taaaatcgct gcttgaaata aaaataactt atcttctata ttaaagtaat tatatttaat
     5761 aaataagagc taatttaaaa aaagatatac agttaaaatc tatttc
//
LOCUS       DAMJYR010000195         5761 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.200, whole genome shotgun sequence.
ACCESSION   DAMJYR010000195 DAMJYR010000000
VERSION     DAMJYR010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5761)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5761)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5761
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.200"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(239..2071)
                     /locus_tag="PC471_003584"
     CDS             complement(239..2071)
                     /locus_tag="PC471_003584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2790667.1"
                     /translation="MSRMGRGPMGKSMGAGQKANDFKGTMRKLIAYLSKFKISIILVI
                     VFAIGSASFSIVGPKILGKATTKIFEGLVSKVSGGNVGIDFNAIGKILTFLLFLYLIS
                     ALFSFIQGFIMSGISQKVSYNLRKEISAKLDRLPMKYFDTKTHGEILSRITNDIDTLN
                     QSLNQSMTQLITSVTTMIGVLIMMLSISGIMTLVAVLILPISMFVISRIIKKSQKYFR
                     YQQEYLGNVNGQVEETYSGQTIVKAFNREDEVIEEFDKLNDSLYNSAWKSQFLSGIMQ
                     PLMMFIGNLGYVMVSILGGWLAIKKTIEVGDIQSFIQYVRNFTQPMTQIAQVANLLQS
                     TAAASERVFEFLEEEEEVQIVENAVSIDGLEGKIDFENVNFGYNPNKTIINDFSVNVK
                     PGQKVAIVGPTGAGKTTIVKLLMRFYDVNSGSILIDGHNIKDFNRSELREMFGMVLQD
                     TWLFSGSIMENIRYGKLNATDEEVIEAAKSAHVHRFIKTLPDGYKMKLNEEASNVSQG
                     QKQLLTIARAILADPKILILDEATSSVDTRTEVLIQKAMDNLMEGRTSFVIAHRLSTI
                     RDADMILVMNEGDIVEQGNHEELLKKGGFYANLYNSQFEEDEAM"
     gene            complement(2064..4310)
                     /locus_tag="PC471_003585"
     CDS             complement(2064..4310)
                     /locus_tag="PC471_003585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011009877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2790668.1"
                     /translation="MFKLIKYLKKSALSIVIIVCLLVIQAVCDLSLPEYTSNIVNVGI
                     QQGGVENSVPSVIRESELNKITLFMDKSSKDKVLDNYTLLNKKDYVKYKDKYPGLKDE
                     SLYELNTKDKDTIDDLNVIFGKAILIVSGLEGDSKEVKAMKAQLMSKLPPQATQSGDV
                     DIFKLLSAMPKEQLDTLTKEMSKGFESMPESMITQSSVSYVRSEYEKIGIDTEKTQNN
                     YILFTGAKMLGIAMISMVATITVGFLAARVAASVGRNLRSGVFRKVMSFSNTEMNEFS
                     TASLITRSTNDIQQIQMLMVMLLRIVFYAPIMAIGGVIKVLNTNTSMAWIIAVAVVAI
                     LSLIVVLFSVVMPKFKLVQKLVDKLNLVSREIITGIPVIRAFSTEKHEEERFDKANKN
                     LTKTNLFVNRVMTCMMPAMMLIMNLITVLIVWRGAYSVDSGAMQVGDMMAFIQYTMQI
                     IMSFLMISMVSIMLPRASVSATRIDEVLVSDISIKDPEKSEAQEFKEDKKGLVEFKNV
                     SFMYPDAEEPILTNISFTAKPGETTAFIGSTGSGKSTLINLIPRFFDVTEGEILLGGV
                     NIKNVSQHDLREKIGYVPQKGVLFSGTIESNLKYGRKDATDEEMRTAAEIAQATEFID
                     SKPEAFKTEISQGGTNVSGGQKQRLSIARAIAKNPEVYIFDDSFSALDLKTDAALRKA
                     LKSKTAESTVLIVAQRISTILNAEQIIVLNEGEIVGIGTHKELLKNCEVYEQIALSQL
                     SKEELANE"
     gene            complement(4378..4839)
                     /locus_tag="PC471_003586"
     CDS             complement(4378..4839)
                     /locus_tag="PC471_003586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDF2790669.1"
                     /translation="MNSNSEEKLNDVFLQLMRIYFIRTHFYFDKLGLYHGQPQILFSL
                     KMKDGQSQKELADKRKVKASTTTVMIKRMEKAGLVERKQDEKDQRVSRIFLTDKGLEI
                     CNKVEEFNKELEREFFLGFSEEEKIVLRRLLMQVRDNLKAGCHKHEDEKYF"
     gene            complement(5144..5710)
                     /locus_tag="PC471_003587"
     CDS             complement(5144..5710)
                     /locus_tag="PC471_003587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HDF2790670.1"
                     /translation="MRKLEEILEYNKSFVENKEYEQYVTSKHPNKKIVVLSCMDTRLT
                     ELLPKAMNLKNGDVKLIKNAGATIMHPFGSIMRSVLVAIYEFDVDEVMVVGHHGCGMC
                     NVDTDKLLGKILDRGISNDVILTLRSAGIDTKQWLHGFDSAEESVKDSIDLIKGHPLI
                     PDGIIVHGLIISPETGKLDVVVNGYEDK"
BASE COUNT         1828 a         1079 c          633 g         2221 t
ORIGIN      
        1 gataatcacc tccaacattt tccttactac atattataat aaaataaaca ctttgtaaag
       61 acataaaatt atgtggctat ctcaaaatat agagaatcct ctttttcatt gttaaaatga
      121 taaaaataca ctttatttca tacacaaaag agtgccttat gaattaaaaa gttcatttta
      181 aggcaccctc atttatttta tcttattaat tacatgtcta tgctaagata gttttttgtt
      241 acattgcttc atcttcttca aattgagaat tgtaaagatt ggcatagaat ccgcctttct
      301 ttagaagttc ctcgtgattt ccttgttcaa ctatatctcc ttcattcatt acaagaatca
      361 tatcagcatc acgtatagtt gataatctgt gggctataac aaaacttgtt ctaccttcca
      421 taagattatc catagctttt tgaatcaata cttctgttct agtatcgaca gaacttgtag
      481 cttcatctaa tattaaaatt ttaggatcag ctaaaatagc acgagctata gtcaataatt
      541 gtttttgacc ttgagaaaca ttacttgctt cttcatttaa tttcatctta tatccatctg
      601 gtaatgtctt aataaatcta tgaacatgtg cagatttagc agcctctata acttcttcat
      661 ctgtagcatt caacttacca tatcttatat tttccataat tgaaccacta aataaccatg
      721 tatcttgaag aaccatacca aacatttctc taagctcact acgattaaaa tctttaatat
      781 tatgaccatc aatcagtatt gaaccgctat taacatcata aaatctcatt aacagtttaa
      841 ctattgtagt tttacctgct cctgtaggtc caacaatagc aactttttga ccaggtttaa
      901 catttactga gaaatcattt attatagtct tgtttgggtt atatccaaag ttgacatttt
      961 caaaatcaat tttaccttct aaaccatcta ttgaaactgc attttcaaca atttggactt
     1021 cttcttcctc ttctaaaaac tcaaatactc tttcagaagc tgctgctgtt gattgcaata
     1081 aattggcaac ctgtgcaatc tgagtcattg gctgtgtaaa gtttcttaca tattgtataa
     1141 atgactgtat gtcaccaacc tcaattgttt tcttaattgc aagccaacct cctaagattg
     1201 ataccattac atatcccaag tttccaataa acatcataag tggttgcatt atacctgata
     1261 aaaattgaga tttccacgct gaattataca ggctatcatt tagtttatca aactcttcaa
     1321 ttacttcatc ctcacgatta aatgctttta ctattgtttg gccactgtaa gtttcctcaa
     1381 cttgaccatt tacatttcct aaatattctt gttgatatct aaagtatttt tgtgatttct
     1441 ttattattct tgaaattaca aacattgata ttggtaatat taaaactgcc actaatgtca
     1501 taattccact tatagataac atcattatca aaacacctat catagttgtt actgatgtta
     1561 taagctgtgt catactttga tttaaacttt gatttaaagt atctatatca ttcgtaattc
     1621 tcgataatat ttcaccatgc gtcttagtat caaaatactt cattggcaat ctatccaatt
     1681 ttgctgaaat ctcttttctt aaattatagg atactttttg agaaattcca ctcattataa
     1741 atccttgtat aaaagagaac agtgcactta tcaaataaag gaaaagtaaa aaggttaaga
     1801 tttttccaat tgcattaaaa tctataccta cgtttcctcc agaaacttta ctcactagcc
     1861 cttcaaatat ctttgtagtt gcctttccta aaatttttgg acctactata gaaaacgacg
     1921 cactaccaat agcaaataca attactagaa ttattgatat tttaaattta gataaatatg
     1981 caattagttt tcgcatagtc cctttaaagt catttgcttt ttgtcctgct cccatagatt
     2041 ttcccatagg acctcttccc attctactca ttagctagct cctcctttga aagttgtgat
     2101 aaagctattt gctcataaac ttcgcaattt tttagaagct ctttatgtgt tcctattcca
     2161 actatttcgc cctcatttaa aacaattatc tgttctgcat ttaagatagt actaattctt
     2221 tgtgcaacta ttagaactgt actttcagca gtttttgatt ttaaagcctt acgaagagct
     2281 gcatcagttt ttaagtcaag tgctgaaaaa ctatcatcaa atatataaac ttctggattt
     2341 tttgctattg ctcttgcaat agaaagtctt tgtttttgac ctccagatac atttgttcca
     2401 ccttgagaaa tttcagtttt aaaagcttcc ggtttagaat ctataaattc tgtagcttga
     2461 gctatttcag ctgctgttct catttcttca tctgttgcgt cttttctacc atattttaag
     2521 ttggattcta tagtaccaga aaacaatact cccttttgag gtacatatcc aattttttcc
     2581 cttaaatcat gttgagaaac attttttata ttaactccac caagtaatat ttctccttca
     2641 gtaacatcaa agaaacgagg aattaaattt ataagagttg acttaccact accagtactt
     2701 cctataaagg cagttgtttc tcctggcttt gctgtaaaag atatatttgt taatataggt
     2761 tcttctgcgt ctggatacat gaacgaaaca tttttaaatt caacaagacc ctttttatct
     2821 tccttaaact cttgtgcctc tgatttttct gggtctttta tgcttatatc tgacactaat
     2881 acttcatcta tacgtgtagc tgaaacggat gctcttggaa gcataataga taccattgat
     2941 atcattaaga atgacattat tatttgcatt gtatattgta taaatgccat catatctcca
     3001 acttgcattg ctccagaatc aacactgtat gcacctctcc aaactataag aactgtgata
     3061 aggttcatta taagcatcat agcaggcatc atacatgtca taactctatt tacaaaaaga
     3121 tttgttttag ttaaattttt atttgcttta tcaaatcttt cttcttcatg tttttcagta
     3181 ctaaaagctc tgataactgg aataccagta attatttcac ggcttactaa gtttaactta
     3241 tcaacaagct tttgaaccag tttaaactta ggcataacca ctgagaataa cactacaatt
     3301 aatgataaga tagcaactac tgctactgct ataatccagg ccattgaagt atttgtgttt
     3361 aaaaccttta tcactccacc tatagccatt ataggtgcat agaatactat tctaagcaac
     3421 ataaccatca acatttgtat ctgttgaata tcatttgtac tacgagtaat taatgaagct
     3481 gtagaaaatt cattcatttc agtatttgaa aatgacataa cttttctaaa tactccactt
     3541 cttaaatttc tacctacact tgcggctact cttgctgcta aaaatcctac tgttattgta
     3601 gctaccatac taatcattgc tataccaagc attttagcac cagtaaataa tatataatta
     3661 ttttgagttt tttctgtatc aatacctatt ttctcatatt ctgaccttac atatgaaaca
     3721 gagctttgtg ttatcatact ctctggcata ctttcaaagc ctttgctcat ctctttagtc
     3781 aatgtatcta actgttcctt tggcattgct gataacaatt taaatatatc aacatctcca
     3841 ctttgagtag cttgtggtgg aagtttgctc attaactgag ctttcatagc ttttacctct
     3901 ttactatcgc cctcaagccc tgatactatt aaaatagctt taccaaaaat aacatttaaa
     3961 tcatctatag tgtctttgtc tttagtattt aactcataaa gagattcatc ttttagacct
     4021 ggatacttat ctttatactt aacataatcc tttttattca gcaaagtata attatctagt
     4081 actttatcct tactactttt atccataaat aaagtaattt tatttaattc actctctcta
     4141 atcacagatg gaacagagtt ttctactcca ccttgttgta tacctacatt tactatattg
     4201 gatgtgtatt ctggtaaaga aaggtcacat accgcttgaa ttaccaatag acagactatt
     4261 attactattg ataatgccga tttctttaaa tatttaatta atttaaacat acatttcttc
     4321 ccttctgtaa tattaattag tctaataaca ttaaattgaa aaacattatt ttaaacttta
     4381 aaaatatttt tcatcttcat gcttatgaca tcctgctttt aaattatctc gtacttgcat
     4441 tagaagtctt ctaagtacta ttttttcttc ttcactaaat cctaaaaaaa actctctttc
     4501 caattccttg ttaaattctt caactttatt acatatttct aaacctttgt ctgtcaaaaa
     4561 aatcctagat actctttggt ccttttcatc ttgttttctt tccacaagtc cagctttttc
     4621 catcctcttt atcataactg tagttgtaga tgcttttact ttccttttat ctgctaattc
     4681 tttttgactt tgcccatctt tcatctttaa agaaaaaagt atttggggtt gaccatgata
     4741 taagcctaat ttatcaaaat aaaaatgagt tcttataaaa tatattcgca ttaattgcaa
     4801 gaaaacatca tttaattttt cttcagaatt gctattcata cttgccctcc ttatttttta
     4861 tattacttag tcggctaagc attattttac tatactgcat gcattttttc aatatattta
     4921 taaaaaattc atataaattt catttattaa atatttttat atcatatagt tataatttta
     4981 ataaataata gtaagatttt taattagtca gctacttata ttttttattg taaagcttaa
     5041 aataaacctt ttcatatatt ttcaaataaa aaagttgtcc caaaataaat tttgaaacaa
     5101 catcatattc aacagtatct tgattatata acattatgat actctactta tcttcatatc
     5161 catttacaac aacgtctaac ttcccagttt caggtgatat aattagacca tgtacaataa
     5221 taccatctgg aattaaagga tgccctttta ttaaatcaat actatctttt acagattctt
     5281 ctgcagaatc gaatccatgt aaccattgtt ttgtatcaat tccagcacta cgaagtgtaa
     5341 gtattacatc attagatatt cctctatcta aaattttacc aagtaactta tccgtatcaa
     5401 cattacacat accacatcca tgatgcccta caaccataac ctcatctaca tcaaattcgt
     5461 aaattgcaac taaaacactt ctcataatac ttccaaaagg atgcattata gtagctcctg
     5521 cattttttat tagtttcaca tctccatttt tcaaattcat agccttaggc aaaagctcag
     5581 ttaatcttgt atccatacaa gataagacta ctattttttt atttggatgt tttgatgtca
     5641 catattgttc atattctttg ttctctacaa atgatttatt atattctaat atctcttcaa
     5701 gttttctcat atgtaatatc accctcttta aaaatttttt tattattata tcactattaa
     5761 a
//
LOCUS       DAMJYR010000196         5733 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.201, whole genome shotgun sequence.
ACCESSION   DAMJYR010000196 DAMJYR010000000
VERSION     DAMJYR010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5733)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5733
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.201"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            202..831
                     /locus_tag="PC471_003588"
     CDS             202..831
                     /locus_tag="PC471_003588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDF2790671.1"
                     /translation="MDKNYTMPIYQKIALDIANKIYTGEIQEDSVLFGRSVLAGKYNV
                     SPETIRRAVKILEDIGVVKSIKGKGVIVLSPDKASSFIKKYRDITNISSYKSTLYNLI
                     DTKSNLENEILDTINKILDYSNRLEIINPLVPVQFTINSNCKYIGQTAAQTKFWQNTG
                     ATIVAIKRGEELIISPGPYIEFLEGDILLVVGDQHIYNSIPMFLYENEK"
     gene            complement(945..2459)
                     /gene="glpK"
                     /locus_tag="PC471_003589"
     CDS             complement(945..2459)
                     /gene="glpK"
                     /locus_tag="PC471_003589"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HDF2790672.1"
                     /translation="MEKKYVMALDQGTTSSRAILFNKKGEIVKIAQKEFNQIYPKAGW
                     VEHDPMEIWGSQSGVMREVIETAGIRPEEIASIGITNQRETTVVWSRYTGKPIYNAIV
                     WQCRRTSEICDELKNKGLEESIKEKTGLLIDAYFSATKVKWILDNVEGAREKAEQGEL
                     LFGTIDTWLIWNLTRGKVHVTDYSNASRTMMYNINDLEWDEDILRELDIPISMLPLVK
                     PSSYVYGHTDERMLSGAKIPIAGCAGDQQAALFGQNCVEEGTAKNTYGTGCFLLMNTG
                     SNIVKSKHGLLTTIAWGVDGKVTYALEGSIFIGGASVQWLRDELKIIESAKDSEMYAN
                     RVEDTNGVYVVPAFTGLGAPYWDMYARGSILGLTRGAKKEHIVRATLESIAYQTKDVL
                     EAMQNDSKLKLKSLKVDGGASNNNFLMQFQSDILNVDIDRPKIVETTALGAAYLAGLS
                     VGFYIGRNEITSKWSVEKEFNPNMSEEKRCKLYKGWKKAVSRALSWEKEDELDI"
     gene            2703..3245
                     /locus_tag="PC471_003590"
     CDS             2703..3245
                     /locus_tag="PC471_003590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDF2790673.1"
                     /translation="MPKILENVKEDILKVSRDMILEEDYSNISIRKIAQRCGISAGTV
                     YNYFNSKQEIIEYITKSEWDLLIRRIEYSNKNTEDYIKKLNIIFTEIRNFINKVHNIQ
                     YNDFLNTFETERFFEMKKHKDDFHEQLSNKVYEALEKESFFNNDKLVCNIIIKMFFSY
                     STYLHIEFEDLKPYIEKLIH"
     gene            3259..>5733
                     /locus_tag="PC471_003591"
     CDS             3259..>5733
                     /locus_tag="PC471_003591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDF2790674.1"
                     /translation="MNHNTSIKNKDSNPKIFLVLTLYLLGIFMGAIDTGIVSPARTII
                     QNSLGVNEKTGIWMITIYTLTYASVIPISGKLADKIGRKYVYLVSIFLFGSGSLICGL
                     SSLFSNFYILLIGRVIQAIGGGGIMPIATAEFGTTFPENKRGMALGLVGATYGIANIL
                     GSSIGSTILSIFGTQNWKWLFFVNLPICLIILIGGVFCIKNNKSESKEKIDKLGTLML
                     ICIIVSLLYGLMNIDFFNFKNSIQDISVYPYLLTFIVLIPIFIFIENRAKDPILSFEY
                     FLNPRILIVLILSLIVGIGMMGMVFVPQYAENALKINAGSGGYFVAILGLFAGIAAPL
                     SGRLIDKFGAKKILLLGFSVSMIGSLYLVLIALKTNTVFSVCVSLIFMGLGMGFTMGT
                     PLNYMMLSNTRLEESNSALATLSLIRSIGTSISPAIMIGFIAHAGLSVQDNIMDLVGK
                     PSTPKIVQLEELENMIDDLKSDPEMAKNLKNVSIPNMNDTSNINMDMTGGKLPKSLLN
                     KVQSADTTNITNITKEISTTMFDEKVPSIITKIQKNVQKGIDGTQSGINGIEDGENKL
                     NSGIKGVQTGIDNMVKARAGINQGIDGVKKGIAGVDKGIKGMEQGLEKQDEAIKELTT
                     AYNKIPTTEKTPTDNENNSNEQNQSNTSNSESDDTKNDSTENKNNNSSENKPNVNEQK
                     ESLNAQIQQLKKTRDDLNTKLQKNKTQKNELSKKLRTMESQKKELQSKLNNSISQKKD
                     MEKSLDTMQQQKKSLQAVLAKTQEVKTEIPKAFDKSKLDYINSIENNRTKIENTLQST
                     LNSGFKQMYITVFCVNLLAFIILLFYK"
BASE COUNT         2127 a          874 c          787 g         1945 t
ORIGIN      
        1 atatctttat atttttataa ttaattcttt atatagtttt aagtataata aaatcatgta
       61 tactaaatgc atgttaaaat attattttaa aacatgcaca atatttgtac atattgtaga
      121 catataaacc tataaattat acaatagtat tgttaagatt atatattttt ttaacataaa
      181 ttttgtattg ggggaaaaac aatggataaa aactatacga tgccaatata ccaaaaaata
      241 gcgttggata ttgcaaataa aatatacacg ggtgaaatac aagaggattc agtactattt
      301 ggacgctctg ttttggctgg taaatacaat gtatctccag aaacgataag aagagctgtc
      361 aaaattttag aggacattgg cgttgttaaa agcataaagg gtaaaggtgt tattgtacta
      421 tctccagaca aagcttcttc ttttataaaa aaatatagag atattacaaa tatatcatca
      481 tataaatcaa cactttataa tttaatagat actaaatcta atttagaaaa tgaaatatta
      541 gatactataa ataaaatttt agactattct aacagacttg aaataatcaa tcctttagtg
      601 cccgttcagt tcaccataaa ttctaattgc aaatatattg gtcaaacagc tgcacaaact
      661 aaattttggc aaaatacagg tgctacaata gttgcaatta aaagaggtga agaacttata
      721 atatctcctg gaccatatat tgaattttta gaaggagaca ttctcttagt agttggtgac
      781 caacacattt acaattctat accaatgttt ttatatgaaa acgaaaaata aattatataa
      841 tttagtgtga ttatataaaa atgcaagatt aacaacaaaa aaagagcttc atattttaca
      901 taaagctctt tttaaattca aataaattta actttgatta atttttaaat atccaattca
      961 tcttcttttt cccagcttaa agctctcgat acagcttttt tccatccttt atataattta
     1021 catctcttct cttcactcat atttggatta aactcttttt ctactgacca ctttgaagta
     1081 atttcatttc ttccaatata gaatccaaca gacaaccctg ctaaataagc agctcctaaa
     1141 gctgtagttt ctactatttt gggtctatct atatctacat ttaatatatc tgattgaaat
     1201 tgcattaaaa aattattatt acttgctcca ccatcaactt ttaatgattt tagttttaat
     1261 tttgaatcgt tttgcattgc ttctaaaaca tcctttgtct gataagcaat tgattccaat
     1321 gtggctctta ctatatgttc tttttttgct cctcttgtaa gtcctaaaat tgaaccccta
     1381 gcatacatat cccaatatgg agctccaaga cctgtaaatg ctggcactac atatacacca
     1441 ttagtatcct ctaccctatt tgcatacatt tcactatctt ttgcagattc aattatcttt
     1501 aattcatctc taagccattg aacagatgct cctccaataa atatacttcc ttctaatgca
     1561 tatgttactt ttccatcaac tccccaagca attgttgtaa gtaacccatg cttagattta
     1621 actatgttac tgcctgtatt cataagcaag aaacaacccg ttccataagt attttttgca
     1681 gtaccttctt caacacaatt ttgaccaaat aatgctgctt gttggtctcc agcacatccc
     1741 gcaataggta ttttagctcc tgataacatg cgctcatctg tatgaccata aacatagcta
     1801 gaaggtttta ctaatggcaa catgcttatt ggtatgtcca attctcgtaa tatatcctca
     1861 tcccattcta aatcatttat attgtacatc attgttctag atgcatttga gtaatctgtt
     1921 acatgtactt ttccacgagt taaattccat attaaccatg tatctatagt tccaaataat
     1981 aactctcctt gttctgcttt ttccctagct ccttctacat tatctaatat ccattttact
     2041 tttgttgctg aaaagtatgc atctattaaa agacctgttt tttcttttat tgattcttcc
     2101 aatcctttat ttttaagttc atcacatatt tctgacgttc tcctacactg ccatactatt
     2161 gcgttataaa taggtttacc agtataccta ctccaaacaa ccgtagtctc tctttgatta
     2221 gttatcccta ttgaagcaat ttcttctggt cttatacctg ctgtttctat aacttctctc
     2281 attaccccac tttgtgaccc ccatatttcc atagggtcat gctctaccca tccagctttt
     2341 ggataaattt gattgaactc tttttgtgca attttcacta tttcgccttt tttattaaat
     2401 agtatagctc ttgaactggt tgttccttgg tctaatgcca ttacatactt cttttccatc
     2461 accatattct ccttatcata taatatttaa tttattaaaa agaaaaattt aatatcacat
     2521 atttcaataa ataatcatac ttaagtacaa atttaatata atagatattt agtaagaaga
     2581 cacttatttt ctatcatatc tatataataa tattatacat tatagcttac atattgatta
     2641 ataaatttta aaggagtata ataaacagtg ttcataataa ttttaataaa agaggtggta
     2701 atatgccaaa gattttagaa aatgtaaaag aagatatatt aaaagtgtct cgggatatga
     2761 ttttagaaga agattactcg aatattagca taaggaaaat agctcaaagg tgtggtataa
     2821 gtgcaggtac agtgtataat tactttaatt caaaacaaga aataatagaa tatatcacaa
     2881 aatctgaatg ggatttattg ataaggcgaa ttgaatactc caataaaaac actgaagatt
     2941 acataaaaaa actaaacatt atttttactg aaataagaaa ttttataaac aaagttcata
     3001 atatccaata caatgacttt ttaaacactt ttgaaacaga gagatttttt gaaatgaaaa
     3061 aacacaaaga cgactttcat gaacagcttt caaataaagt atatgaagct ttagaaaaag
     3121 aaagtttttt taataatgac aaattagtat gcaatatcat aataaaaatg tttttttcat
     3181 attcaacata tcttcatata gaatttgaag atttaaaacc ctatattgaa aaattaatac
     3241 attaaggtgg tgtttacctt gaatcataat actagtatta agaacaagga cagtaatcca
     3301 aaaatatttt tagtacttac gctttatcta ttaggtatat ttatgggagc tattgatact
     3361 ggaatagtat caccagcaag aacgattata caaaatagcc ttggagtaaa tgaaaaaacg
     3421 ggaatttgga tgattaccat atacacatta acatacgctt ctgtaattcc tatctctgga
     3481 aaattagcag ataaaatagg aagaaaatat gtgtatctag ttagtatatt tctatttggt
     3541 agtggttcat taatatgtgg attatcatca ctattttcca atttctatat tttattaatt
     3601 ggtcgtgtta ttcaagcaat tggtggtggt ggaatcatgc caattgctac agcagaattt
     3661 ggaactacct tcccagaaaa taaaagaggc atggctttag gtttagttgg ggcaacatat
     3721 ggtattgcca atattttagg ttcaagcatt ggtagcacaa ttctaagtat ctttggaact
     3781 caaaattgga agtggctatt ttttgtaaat cttccaattt gtttaatcat acttattggt
     3841 ggagttttct gtattaaaaa caataagtct gaatctaaag aaaaaataga taagcttggt
     3901 acattgatgt tgatatgtat aatcgtatca ctattatatg gtcttatgaa tatagacttt
     3961 tttaatttta aaaattctat acaagatata tcagtttatc catacttatt gacatttatt
     4021 gtacttatac ctattttcat atttattgaa aatagagcta aagaccctat tttaagcttt
     4081 gaatattttt taaatccgag aatattgata gtacttattt tatctttaat tgttggtatt
     4141 ggaatgatgg gtatggtatt cgttccacag tatgctgaaa atgcattaaa gataaatgct
     4201 ggttctgggg gatattttgt agctatattg ggattatttg caggtattgc tgctccttta
     4261 agcggaagac ttattgacaa gtttggggca aaaaaaatac tattactagg attttctgta
     4321 tctatgatag gctctcttta cctagtttta attgctctaa aaaccaatac tgtatttagt
     4381 gtatgtgtaa gtttaatatt tatgggatta ggaatgggat ttactatggg aactcctcta
     4441 aactatatga tgttatcaaa tacaagatta gaagaatcta attcagcact tgcaacatta
     4501 tccttgatac gctcaattgg tacttcaatt tctccagcta taatgattgg atttatagca
     4561 catgcaggat tatctgtgca agataatatt atggacttag ttggaaagcc ctcaactcct
     4621 aaaatagttc aacttgaaga acttgaaaac atgatagatg acttaaaatc agaccctgaa
     4681 atggcaaaaa atttaaaaaa tgtctctatc ccaaatatga atgatacttc aaatataaat
     4741 atggacatga caggtggcaa acttccaaaa tccttactta ataaagttca gtcagcagat
     4801 acaaccaaca ttaccaatat aacaaaagaa atatcaacaa caatgtttga tgaaaaagtt
     4861 ccatctataa ttactaaaat acagaaaaat gtacaaaaag gaatagatgg aacacaaagt
     4921 ggaataaacg gtattgaaga tggtgaaaat aaactaaata gtggtattaa aggagttcaa
     4981 actggtattg acaatatggt aaaagctaga gctggtatta atcaaggaat agatggtgta
     5041 aaaaaaggta ttgctggtgt tgataaaggt ataaaaggta tggaacaagg tcttgaaaaa
     5101 caagatgagg ccatcaagga acttacaact gcttataata aaatacctac tacagaaaaa
     5161 actcctactg ataacgaaaa taactcaaat gaacaaaacc agagtaatac cagtaattct
     5221 gaatcagacg acactaagaa tgattctaca gaaaataaga ataacaattc cagcgagaat
     5281 aaaccaaatg taaatgaaca aaaagaaagt ttaaatgctc aaatacagca actaaaaaag
     5341 acaagagatg acttaaatac aaagttacaa aaaaataaaa ctcaaaagaa cgaattaagt
     5401 aaaaaactaa gaacaatgga atctcaaaag aaagagctac aaagcaagtt aaataattct
     5461 ataagccaaa agaaagacat ggaaaaaagc cttgatacaa tgcaacagca aaaaaaatca
     5521 ctccaagcag ttttagcaaa gacacaagag gtaaaaactg aaataccaaa ggcttttgat
     5581 aaatcaaaat tggattatat aaattccata gaaaataata gaacaaaaat agaaaacaca
     5641 ctacaatcta ctctaaactc aggatttaaa cagatgtata taacagtatt ctgtgtaaat
     5701 ctactagcat ttataatatt attgttctat aaa
//
LOCUS       DAMJYR010000197         5703 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.202, whole genome shotgun sequence.
ACCESSION   DAMJYR010000197 DAMJYR010000000
VERSION     DAMJYR010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5703)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5703)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5703
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.202"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(286..969)
                     /locus_tag="PC471_003592"
     CDS             complement(286..969)
                     /locus_tag="PC471_003592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HDF2790675.1"
                     /translation="MTSKSQMSESFFLCSLLAITGGFLDIYTYVVRGKVFANAQTGNI
                     VLFGLNIAEGNVKESFYYLIPILAFMLGVFIAEKIRKYFTNNKTKIHWRQVIIIIEMI
                     TILIVSFIPRGRFNMFVNVAISFICSMQVESFRKVNGNSFATTMCTGNLRSATETLFH
                     YIHTKNIFMIKKSLQYYAIIIFFISGAILGTFFTKIFVEKSILICFFILAVVFGIMFI
                     NKDDSFNEN"
     gene            complement(1294..1884)
                     /gene="zapA"
                     /locus_tag="PC471_003593"
     CDS             complement(1294..1884)
                     /gene="zapA"
                     /locus_tag="PC471_003593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HDF2790676.1"
                     /translation="MNKVMVKIHGAEYPMVGDKSEKFMISIADFVDKEMDKITRQNPK
                     LSLSVAAILTALNISDLLFECSDENEKLIKANEELSKKVGASNEELQLEIKSLKLTIA
                     EKEAENREAETKMKELIEIIENKKQEIFELSNTTEGSRAELDAYKNKIEELSTQLEEA
                     NERATIAENLASEFQNKAYDLQLKCTGLNNDAKNVE"
     gene            complement(1909..4302)
                     /locus_tag="PC471_003594"
     CDS             complement(1909..4302)
                     /locus_tag="PC471_003594"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HDF2790677.1"
                     /translation="MLVSLKWLRDYVDIDMDVKEFADKMTMTGTKVETIDYYGEEIEN
                     ILVGKILEIKQHPNADKLVVTKVDIGDKVVQIVTGATNISEGDYIPVAVNGSKLPGGV
                     EIKQTDFRGELSDGMMCSAAELGIDEHYIEEYKRGGIYILDHEDSYELGKDIKDVLGL
                     KDALIDFELTSNRPDCKCMMGIAREAAATIGTKVKYPEIEVKESDEEIDFKVEIDNPD
                     LCRRYVARMVTDVKIEPSPYWMQRRLTEAGVRPISNIVDITNFVMLELGQPLHAFDIN
                     QVETGRIVVRNAKDGEKLVTLDDVERTLDKDMLVITNGEKSLGLAGVMGGANSEITSN
                     TKTVLFESANFKPENIRMTAKKVGIRSEASSRNEKDLDPNLAEIAANRAAQLVEMLGA
                     GKVLKGVVDVYPNKPEPKKLVVNPQRINHLLGVDVPMEQFVGILESLEFKCNLVANDK
                     LEIDVPSFRTDMEQEADVWEEIARIYGFENIPSVQLEGNTTAGIKTSKQKFMDALKDN
                     STAVGLNEILTYSFVSPKGVDKIRVPEGNAKRNFVKLLNPLGEETSVMRTTLIPNMLD
                     VLSTNVSHKIEEVSAFECGHIFIPQDSELPKEENRMCVGMYGKDVDFFALKGTIETIL
                     VNVGFKGYEIEPQDNNTTFHPGRCAKIVYNNKYVGTLGELHPDVIENYNLGQRVYVAE
                     IDIDFVFDNSDRTKNYVPLPKYPSTSRDIALIVKDDVFVKQIEDIIKENGQGLVESYK
                     LFDVYKGSQIEAGYKSIAYSITYRSKDKTLTDEDVAKVHDKILSELSEKLNANLRSN"
     gene            complement(4319..5338)
                     /gene="pheS"
                     /locus_tag="PC471_003595"
     CDS             complement(4319..5338)
                     /gene="pheS"
                     /locus_tag="PC471_003595"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000388219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HDF2790678.1"
                     /translation="MQEKLLALREAALAEIKEAQSIESVESLRVKYLGKKGEITAILK
                     EMGKLSAEERPVVGKVANEVRENIELSINSKKEEINAIEKERKLKEEVIDVTQPGKVL
                     KVGKKHPITQIIDEVTDIFIGMGFSIAEGPEVETVENNFDALNAPKDHPSRDMSDTFY
                     INDGVLLRTQTSPVQVRTMRSQELPIKVIAPGRCFRSDSPDATHSPMFHQIEGLVVGK
                     DVTMAEFKGTMDIFVEKLFGSDIKTKFRPHNFPFTEPSAEVDVTCFKCGGKGCPMCKY
                     EGWIEILGSGMVHPNVLRNCGIDPEVYSGFAFGVGVERLAMLKYEIDDIRLLFENDMR
                     FLNQF"
     misc_binding    complement(5388..5622)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
BASE COUNT         1743 a         1104 c          608 g         2248 t
ORIGIN      
        1 acttgaaata cttctattaa tctatatatt gcctactata cttaagacat tttaattaat
       61 ttatacactg tttactatac ttgaaatact tctattaatc tatatattgc ctactatact
      121 taagacattt taattaattt atacactgct tactatactt aaaatacttc tattaactta
      181 tacattgctt actatatttt aaattcttta attaatctat acaatatttg ctatatataa
      241 agtatatcaa ataacagctt catttttttg taaatttaca gaaagttaat tttcattaaa
      301 tgaatcatct ttattgataa acataattcc aaagaccact gccaaaataa aaaaacatat
      361 tagtattgat ttttccacga aaatttttgt aaaaaaagtt cctaaaatag ctccagatat
      421 aaagaatata ataatagcat aatactgtaa actctttttt atcataaaaa tattcttagt
      481 atgtatataa tgaaataaag tttctgtagc acttcttaaa tttccagtac acattgtcgt
      541 tgcaaaacta ttcccattta cttttctaaa actctctact tgcattgaac aaataaaaga
      601 aatagcaaca tttacaaaca tattaaatct tcctctagga ataaatgaaa caataagtat
      661 tgtaatcatt tcaattataa tgataacctg acgccaatga atctttgtct tattatttgt
      721 aaaatacttt cttatcttct cggctataaa tacacctaac ataaatgcca aaataggaat
      781 caaataataa aatgattcct ttacattacc ttctgctata ttaagcccaa ataaaactat
      841 atttccagtt tgtgcattag caaagacttt acctcttacc acataagtat aaatatccaa
      901 aaatcctcct gttattgcca acaaagaaca taaaaagaat gattcagaca tctgactctt
      961 gcttgtcata tataaacctc tcgctttcta atgatagata caaaatcaaa atcattttag
     1021 attctatttt tcattgatat aaaaaaatta tttatattta aatattgtct atatttatac
     1081 attatttgta tttatatagc attcatattt atttatattt aaacattatc tatatctata
     1141 tagcatttat atttatttac atttaaacat tatctatatt tatatagcat ttatatttat
     1201 ttacatttaa acattatcta tatttatata gcatttatat ttatacatta aaagaagctg
     1261 tctcaaaaaa caagagacag caaatctttt agtttattcc acattttttg catcattatt
     1321 taaccctgta cattttaact gtaaatcata agctttgttt tgaaactcag aagctaagtt
     1381 ttcagctata gtagctcttt catttgcttc ttcaagttga gtacttaact cctcaatctt
     1441 gtttttataa gcatctaact ctgctctaga accttctgtc gtattactaa gttcgaatat
     1501 ttcttgcttt ttattttcta taatctcaat taactctttc atcttagttt cagcttcacg
     1561 attttctgct tctttttctg ctatcgtaag ctttaaagat ttgatttcta attgaagctc
     1621 ttcattagat gctccaacct tcttactcaa ttcttcattc gctttaatca acttctcatt
     1681 ctcatctgaa cattcaaata aaaggtctga tatatttaat gcagttaata tcgcagcaac
     1741 tgataagctc aatttcgggt tttgacgtgt tattttatcc atttctttgt ctacaaaatc
     1801 agcaatacta atcataaact tctctgattt gtctccaacc attggatatt cagcgccatg
     1861 gattttaacc attactttgt tcatcctaac ctcccccgac attagttgtt aatttgatct
     1921 taagttagca tttaattttt cgcttaattc acttaatatc ttatcatgca ccttagctac
     1981 atcttcatct gttagagttt tatctttact tctataagtt attgaatatg caattgactt
     2041 gtatccagct tctatttgag aacctttgta aacatcaaat aacttgtagc tttctactaa
     2101 tccttgacca ttttccttta ttatatcttc tatttgtttt acaaatacat catctttaac
     2161 aataagagct atatctcttg atgtagatgg atattttggt aatggcacat aattctttgt
     2221 tctatcagaa ttatcaaata caaagtcaat atctatttca gcgacataaa ctctttgacc
     2281 tagattgtag ttttctataa catctggatg caattcacct aatgtaccta catatttatt
     2341 attatacact attttagcac atctaccagg gtggaaggta gtattattat cttgaggttc
     2401 aatttcataa cctttaaatc cgacatttac aagtatagtt tctatagttc cttttagtgc
     2461 aaagaagtca acatctttac catacatacc tacacacatt ctattttctt ccttaggtaa
     2521 ttctgaatct tgtgggatga atatatgtcc acactcaaat gcagatactt cttctatttt
     2581 atgagataca ttagttgata aaacatctaa catatttggt attaaagtag ttctcattac
     2641 agaagtttct tctcctaatg ggttaagtaa tttaacaaaa tttctctttg cattaccttc
     2701 tggtactctt atcttatcta ctcccttagg gcttacaaat gaatatgtta atatttcatt
     2761 taatcctaca gcagttgaat tatcttttaa agcatccatg aatttttgtt ttgaagtttt
     2821 aattcctgct gtcgtatttc cttctaattg tacagaaggt atattctcaa atccataaat
     2881 tctagctatt tcttcccata catcagcttc ttgttccata tctgttctaa agcttggtac
     2941 atctatttct aatttatcat tagctaccaa attacattta aactctaatg attctaaaat
     3001 tcctacaaac tgctccattg gtacatctac acctaatagg tggttaattc tttgaggatt
     3061 tactaccaat tttttaggtt ctggtttatt tggatataca tctacaacac cttttaaaac
     3121 ttttcctgct cctaacattt caacaagttg tgcagctcta tttgctgcta tctctgcaag
     3181 attagggtct aagtcttttt catttcttga agatgcttct gacctaatac caactttttt
     3241 agctgtcatt cttatgtttt ctggtttgaa attggcactt tcaaaaagta cagtcttcgt
     3301 attagaagtt atttctgagt tagcaccacc cattacacca gctaaaccaa gtgatttttc
     3361 tccatttgtt ataactagca tatctttgtc taatgttctc tcaacatcat ctaatgttac
     3421 aagtttctct ccatctttag catttcttac tactattctt ccagtctcta cttgatttat
     3481 atcaaaagca tgaagtggtt gaccaagctc taacattacg aagtttgtta tatcgactat
     3541 gttacttata ggtcttactc ctgcttctgt aagtcttctt tgcatccaat atggagaagg
     3601 ttctattttt acatctgtaa ccattctagc aacatatctt ctacataaat ctggattatc
     3661 tatctcaact ttgaaatcta tctcttcgtc actttctttt acttcgattt caggatattt
     3721 tacttttgtt cctatagttg cagctgcttc tctagctata cccatcatgc atttacaatc
     3781 aggtctgttt gaagttaatt caaaatctat taaagcatct tttaatccta aaacatcttt
     3841 tatatctttt cctaattcat aagaatcttc gtggtctaaa atatatatac cacctctttt
     3901 atactcctca atgtaatgtt catctatacc tagttcagct gctgaacaca tcataccatc
     3961 tgataattca cctctgaaat cagtctgttt gatttcaact cctccaggta acttagaacc
     4021 atttacagct actggaatat aatctccttc tgatatattt gtagctcctg taactatttg
     4081 aacaacttta tctccaatat ctactttagt tacaaccaac ttatcagcat ttggatgttg
     4141 ttttatttct aaaatctttc caaccaatat attttctatt tcttcaccat aataatctat
     4201 tgtttcaact ttagttcctg tcattgtcat tttatcagcg aactctttta catccatgtc
     4261 tatatcaaca tagtctctaa gccattttaa agatactaac atctacatac cctcctaatt
     4321 aaaattgatt taagaatctc atatcatttt cgaataataa tctaatatca tctatttcgt
     4381 atttaagcat tgcaagtctt tcaaccccaa ctccaaatgc aaatccactg taaacttctg
     4441 ggtctattcc acaatttcta agcacatttg gatgaaccat acctgaacct aatatttcta
     4501 tccaaccttc atatttacac attgggcaac ctttaccacc acatttgaaa caagtaacat
     4561 caacctctgc acttggttct gtaaatggga agttgtgagg tctaaactta gttttgatat
     4621 cagaaccaaa caatttttca acgaagatat ccatagttcc tttaaattct gccatagtaa
     4681 catcttttcc aacaacaagc ccttctatct gatggaacat tggtgagtgt gtagcatctg
     4741 gcgagtctga cctaaaacat ctacctggtg caattacttt tattggtaac tcttgacttc
     4801 tcatagttct tacttgaact ggagatgttt gagttctaag taatacccca tcattgatat
     4861 agaatgtatc gctcatatct cttgatggat ggtctttagg agcgtttaat gcgtcaaagt
     4921 tgttttcaac agtctcaact tctggccctt ctgctataga gaatcccatt ccgataaata
     4981 tatctgttac ttcatctata atttgagtta ttggatgctt cttcccaacc tttaaaactt
     5041 ttcctggttg agtaacatct atcacttcct cttttaattt tctttctttt tcaatagcat
     5101 ttatttcttc ttttttagaa tttatgctaa gttcaatgtt ttctcttacc tcattggcaa
     5161 ccttaccaac tactggtctt tcttcagcag ataatttacc catttcttta agtatggcag
     5221 ttatctcacc tttttttcct aagtacttaa ctcttaaact ttctacactt tctatgcttt
     5281 gtgcttcttt tatttcagcc aaagctgctt cacgtaaagc aagtaatttt tcttgcacac
     5341 atatcaccct ttcgtttttt tgtataaaaa aagcccctca atccctgtct agggacgaaa
     5401 ggctatagtt tcgcggtacc accctagtag tttaacaatt taaaaatcat caaaccacct
     5461 ctaacaaact taacgcgttt ttgacgtcaa agcctactgt aaaatttcag ctctgttact
     5521 ccagagggaa cttctcatga cctcttagaa aacactccca gcctaggtgt ttatctcttt
     5581 actaagttta atcaaaatac tttcctcttt cattgcattt acaatattat attgtttatt
     5641 ttagtattat atcatttcca ttcaaattat gtgactattt ttaatatata tccaatacat
     5701 aat
//
LOCUS       DAMJYR010000198         5702 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.203, whole genome shotgun sequence.
ACCESSION   DAMJYR010000198 DAMJYR010000000
VERSION     DAMJYR010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5702)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5702)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5702
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.203"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            28..879
                     /locus_tag="PC471_003596"
     CDS             28..879
                     /locus_tag="PC471_003596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HDF2790679.1"
                     /translation="MKNIISAKELISKLEKNDNLVVIDCRFDLINRTYGIDSYKKGHI
                     KGSFILDIDKDLSSPTKEHGGKNPLQDPLILKEKLEKMGVDNDTTIVTYDDGDLNGAC
                     RLFFQLKHLGLKNVYVLDGGITSFVKEGGELEEKVNIPSCTGKEIRPNINNDLVVPME
                     YVKSKLYKKDTVIIDCRANERYQGLVEPAYSKAGHIPSAKNYFCKDLIKSDFENGSLK
                     DIEFLKKFFKDLNNYDEVILSCGSGISACVNSLALRELDIPHKIYIGSFSDWISYDDN
                     EIKTGQE"
     gene            complement(1012..2625)
                     /gene="ggt"
                     /locus_tag="PC471_003597"
     CDS             complement(1012..2625)
                     /gene="ggt"
                     /locus_tag="PC471_003597"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HDF2790680.1"
                     /translation="MKFNAYQHKYSSIRNVVYAKNGAVATSTPLASQAGLEILKKGGN
                     AVDAAVATAATLAVVEPTSNGIGGDAYALVWIEEEKRLYGLNSSGFAPENMELKNYNN
                     MKEMPKYGFGAVTVPGIPAAWSELNKKYGKLSLMECLSPAINYAREGYVVSPNVAKVW
                     KKSYELYEKELIGEEFKPWFDTFSKDGKAPEAGDIFICEEQASTLEEIANTQAESFYR
                     GRLADKIDEYSKKFNGAIRKSDLECFYPTWVEPISTEYKGYKIFEIPPNGHGITVLMA
                     LNILKELELEGNIENVEDIHKIIESLKLAFADSKTYVTDIEHMKVKIQELLSQEYAKK
                     RSLLIDNKEALYPTAGEPYCGGTVYLCTADKDGNMVSYIQSNYINFGSGIVIPRTGIA
                     LHSRGNNFNLDPKHHNVVKPFKKPYHTIIPGFLGKEDKAIGPFGVMGAFMQPQGHIQV
                     LTNMIDFGLNPQEALDAPRWQWIKGKEIEVEPEMPKHIIDSLIEKGHEIKVIHDTVDM
                     GRGQIIFKTEQESYICGTESRCDGHIAVY"
     gene            complement(2850..4787)
                     /locus_tag="PC471_003598"
     CDS             complement(2850..4787)
                     /locus_tag="PC471_003598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2790681.1"
                     /translation="MTLFRIAMQNVKNSFFNYLMYFISIVFSVFVFFSFKSIEYNEAL
                     SSLGEKTRMSINTSSIVIVVFVFLFIYYSNSFFFNRRRREVGTYSLLGMRKNQIGKIF
                     LYETFLMGIVAILIGIFLGLLFSKLMTMMLVKLMNEMIVVNMNLSIKALVQTLAVFLI
                     IFIVIGIRNTIVIRNKRIIELFNKYPEKYKFKKFIKLKCVLGVLLIAISYLMSISYFI
                     AENIMLSVFILVTIIPGTFLFFSSTMYMIIDAVKKRKSFYYKGQNLIAFSELGFKLKS
                     NSSVLAVIAILIATSVTMLGFTISLYYDIDRNISENYKYSYTINLGNSYVNNEIDKLL
                     YKYKKDNKIIFDKTIELIDKDINIEMYYKNGNSYIEDRTSVDIIRESDFKKLRQYQNN
                     NYEELTSKESVYYVSDSYKKIFFKDFEVEKINLHIKDTNEYDINLFKVQKSILEPEIN
                     SQATFDLIVAKDDVFNQLKLHGNSRLIRLIDIKNEKKELDLTLKLKNIVSENMKFTYP
                     FNFTSSIESYNNLIKLSGLMLFIGIFLSVVFLLCTGSIILFKQLSNIYDDKERYIMLI
                     KLGANNKDIEKIISKQLKVIFLMPLVVGTVHNLFAMSIAQKFIPRSLLVPIIITLVIY
                     FIGYFIYYFLTLKYALNMIKE"
     gene            complement(4774..5541)
                     /locus_tag="PC471_003599"
     CDS             complement(4774..5541)
                     /locus_tag="PC471_003599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003722224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2790682.1"
                     /translation="MEEILSVENIKKEYGRKGSKHEALRGITFKVYKGEFVGIMGSSG
                     AGKSTLLNIISTIDLPSSGDIYINGKNTIKMKQNELADFRRDNLGFVFQDSNLLDTLT
                     IKENIMLPLSLKNERVSVIENRIKEISKELNIESILDKYPGEVSGGQKQRGAVCRAIA
                     TKPSLVLADEPTGALDSKSARDLLNCLLKLNKDSNKTILMVTHDAISASFCNRILFIK
                     DGIIFTEIVKGESNREFYNKIVNTVSLIGGVNKNDFI"
BASE COUNT         1919 a          916 c          641 g         2226 t
ORIGIN      
        1 ataatataaa cacaggaggt acattctttg aaaaacatta tttcagcaaa agaacttata
       61 tcaaaactag aaaaaaatga taatctagtt gtaatagatt gtagatttga tcttatcaat
      121 agaacttatg gtattgattc ttataaaaaa ggacacatca aaggcagttt tatcttagat
      181 atagacaagg atttatcatc tcctacaaaa gaacatggtg gtaaaaatcc tttacaagac
      241 cctttaatac taaaagaaaa attagaaaaa atgggtgttg ataatgacac tacgattgta
      301 acttatgatg atggagattt aaatggagct tgcagattat ttttccaatt aaaacatttg
      361 gggcttaaaa atgtctatgt actagatggg ggaataacat ctttcgtaaa ggaaggtgga
      421 gaattggaag agaaagtgaa tataccaagt tgtacaggta aggaaattag accaaatata
      481 aacaatgatt tagttgttcc aatggaatat gttaaatcta aattatacaa aaaagatact
      541 gtaataatag attgccgtgc taatgagaga taccagggtt tagttgaacc tgcttactca
      601 aaagctggtc atataccaag tgcaaaaaat tatttttgta aggatttaat aaaaagtgac
      661 tttgaaaatg gctcacttaa agatatagaa tttttaaaaa aattctttaa agatttaaat
      721 aattatgatg aagtaatttt atcttgtggt tcagggatat ctgcttgtgt taacagtctt
      781 gctcttagag aactggatat acctcacaag atttatattg gtagctttag tgactggatt
      841 agttatgatg acaatgaaat caaaactggt caagaataag gctcataatc tatataaaat
      901 cagcatatga tagcttaaat tttgtaaagg cgtcagtcat tgtagacttt cgcctttact
      961 tattatttca tatactcttt aaatttatta tatatatttt agttgataat attaataaac
     1021 tgcaatatga ccatcacatc ttgattccgt cccacaaata taactttcct gttctgtttt
     1081 aaatattatt tgacctcgtc ccatgtcgac agtatcatga attactttta tttcatgacc
     1141 cttttcaatt aatgaatcta ttatatgctt tggcatctct ggttcaactt ctatttcttt
     1201 tccttttatc cactgccatc ttggagcatc tagcgcctct tgtggattta atccaaaatc
     1261 aatcatattt gttaacactt gaatatgtcc ttgtggttgc ataaaagctc ccattacacc
     1321 aaatggacct attgctttat cttctttacc taaaaatcca ggtattatag tatgataagg
     1381 ttttttaaat ggttttacta cattgtggtg tttagggtct aaattaaaat tatttcctct
     1441 actatgtaaa gcaatcccag ttcttggtat cacaattcct gaaccaaaat ttatgtaatt
     1501 gctctgtata tatgaaacca tatttccatc cttatctgca gtacaaaggt atactgtacc
     1561 accacaatat ggctctcctg cagttggata caaagcctct ttattgtcaa tcaataaact
     1621 tcttttttta gcatactctt ggcttagtaa ttcctgaatc tttaccttca tatgctctat
     1681 atcagttaca tatgttttag aatctgcaaa agctaatttt aaactctcta taattttatg
     1741 tatgtcttct acattttcta tatttccttc taattccaat tcttttaata tgttaagtgc
     1801 cattaaaact gttattccat gaccatttgg tgggatttca aatattttat atcccttata
     1861 ttctgtactt ataggttcta cccatgttgg ataaaaacac tctaagtcac tctttcttat
     1921 agccccatta aattttttag aatattcatc tattttatca gcaagtcttc ctctataaaa
     1981 gctttcagcc tgagtatttg ctatctcttc taatgtactt gcctgttctt cacatataaa
     2041 tatatctcca gcctctggag ctttaccatc tttagaaaat gtatcaaacc atggtttgaa
     2101 ctcttctcca ataagttctt tttcatagag ttcatatgat tttttccata ccttagctac
     2161 attaggagat actacgtatc cctctcttgc atagtttatc gctggagata agcattccat
     2221 taagctaagt ttaccatatt ttttatttaa ttctgaccaa gctgctggta ttccaggtac
     2281 agttactgct ccaaacccat attttggcat ttctttcata ttattataat tttttaattc
     2341 catattttct ggagcaaagc cactagaatt taaaccatac aatcgttttt cttcctctat
     2401 ccaaacaagg gcatatgcgt ctcctccaat cccattgctt gtaggttcta ctactgctaa
     2461 tgtggcggct gttgcaactg ctgcatcaac agcatttcca ccctttttta gtatttcaag
     2521 tccagcttga gatgctaatg gtgttgatgt tgcaactgct ccatttttag catacacgac
     2581 atttcttatt gatgagtatt tatgttggta ggcattaaac ttcataaatt tcagctcctc
     2641 gtcatttttt atttgatatt ctgcttagat ttattatata acatcaagta atgtaactga
     2701 tacaaaatga tataaaatat agactttttc ctaactttaa ttttgtcttt aaataaatat
     2761 cttaaactca attctagtat tttctatctg cacttaaata agttatttta ttcaaaacat
     2821 atatctaatt cttttgactt aaaaataatt tattctttta tcatgtttag agcatatttt
     2881 aaagttaaaa aataatatat aaaatatcct ataaaataaa tgactagagt tatgataatt
     2941 ggtacaagta atgaccttgg tataaacttt tgtgctatag acatagcaaa taaattatga
     3001 actgtaccta caactaatgg cattaaaaat ataactttta actgttttga tattattttc
     3061 tcaatatctt tattatttgc tccaagtttt attaacataa tatatctttc cttatcatca
     3121 tatatatttg ataattgttt aaataagatt atactacctg tacataaaag aaatactaca
     3181 gacaaaaata tgcctataaa cagcattaga ccacttaatt ttatcaagtt attataactt
     3241 tcaatacttg atgtaaaatt aaaaggataa gtaaatttca tattttcact tactatattt
     3301 tttaatttta aagtcaaatc cagttctttt ttttcatttt ttatatcaat taatcttatt
     3361 aatctagaat ttccatgtaa ttttaactga ttaaatacgt catcttttgc tacaattaaa
     3421 tcaaacgtag cttgtgaatt tatttctggc tccaagatgc ttttttgtac tttaaacaga
     3481 tttatatcat actcatttgt atctttaata tgcaagttta ttttttcaac ctcaaagtct
     3541 ttaaaaaata tttttttgta tgaatcagaa acatagtaca cagattcctt tgaagttaat
     3601 tcttcataat tgttattctg atattgtctt aattttttaa aatcactttc tctaataata
     3661 tctacagacg ttctatcttc tatataacta tttccatttt tataatacat ttcaatattt
     3721 atatctttgt ctataagttc tattgtttta tcaaagatta tcttattatc ttttttatat
     3781 ttatataata atttgtcaat ttcgttattt acataagaat ttcctaaatt tatggtataa
     3841 ctgtatttat aattttcact tatgttccta tctatatcgt aataaagtga tatggtaaac
     3901 cctagcatag ttacacttgt agctattaat attgctatta cagccaatac actactattg
     3961 ctctttaatt taaatccaag ttcagaaaat gcaattaaat tctgtccctt ataataaaaa
     4021 ctttttctct tcttaacagc atcaataatc atatacatcg ttgatgaaaa aaataaaaaa
     4081 gttccaggta ttattgtaac taatataaaa actgaaagca taatattttc agcaataaaa
     4141 taactaattg acattaaata agaaatagct attaataaaa caccaagtac acattttaac
     4201 tttataaatt ttttaaactt atacttttct ggatatttat taaataactc tataattctc
     4261 ttatttctga taactatagt atttcttatt ccaataacta tgaatattat taaaaatacc
     4321 gctagtgtct gaactaaggc ctttatactt aagttcatat ttactactat catttcattc
     4381 attaatttta caagcatcat agtcataagc tttgaaaata acagtcctaa aaatatacct
     4441 attagtattg caactatacc catcaaaaaa gtttcatata aaaatatttt tcctatctga
     4501 tttttcctca ttccaagtaa actataagtt cctacttctc gcctacgtct attgaaaaaa
     4561 aatgagtttg aataatatat aaataaaaat acaaatacca ctattacaat tgaacttgta
     4621 ttaatactca tcctagtttt ctctccaagt gaactcaacg cttcattata ctctatagat
     4681 ttaaaactaa aaaatacaaa tacactaaat acaattgaaa taaagtacat taaataatta
     4741 aaaaaactat tctttacatt ttgcattgca attctaaata aagtcatttt tattaactcc
     4801 tccaattaag gaaactgtat ttactatttt attatagaac tctctgttac tttctccctt
     4861 tacaatttct gtgaatatta ttccatcctt tataaagagt attctattgc aaaaactcgc
     4921 acttatagca tcatgagtta ccatcaatat tgttttattg ctgtctttgt ttaattttaa
     4981 tagacaattc aacaaatccc tagcagattt agaatctaat gctcctgtag gctcatctgc
     5041 aagtactaat gatggttttg ttgctatagc tctacataca gctcctcttt gcttttgtcc
     5101 accagaaact tcgccagggt atttatctaa tatagattct atatttaatt cttttgaaat
     5161 ctcttttatt ctattttcta ttactgatac tctttcattt tttaaagaaa gaggcaacat
     5221 tatattttct tttattgtaa gtgtatccaa aagattagaa tcttgaaata caaagcctag
     5281 attatcacgt ctaaaatctg ccaattcatt ttgcttcatt ttaatagtat tctttccatt
     5341 tatataaata tctcctgatg aaggtaaatc tattgtagaa ataatattta aaagtgttga
     5401 tttaccagca ccggaagacc ccataatacc aacaaattct cctttataaa ccttgaaagt
     5461 tattcctctt agagcttcat gttttgaacc ttttcttcca tactcctttt ttatattttc
     5521 aacagataat atttcttcca tagttaataa tcacctttca tcacaattag ttatttatca
     5581 attctcacaa atatattata tatattaaaa tggcttttaa acatttaatt atattaattt
     5641 aaggttacat tttcgtaagt attaattata tttcttcaaa tttattgcaa ttatgtttaa
     5701 aa
//
LOCUS       DAMJYR010000199         5609 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.204, whole genome shotgun sequence.
ACCESSION   DAMJYR010000199 DAMJYR010000000
VERSION     DAMJYR010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5609)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5609)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5609
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.204"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            240..1067
                     /locus_tag="PC471_003600"
     CDS             240..1067
                     /locus_tag="PC471_003600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIM24 family protein"
                     /protein_id="HDF2790683.1"
                     /translation="MDLSKKVKILENASDGKSEFEILEFEKLEGASSPLMAMGLYFAR
                     EAGLKARQIKIKLNNSSIKTEAGALYYYKGNISSETKIGGVGGLFKKGISGALTNESM
                     MKPVYKGTGEVYLEPSFRHYLFMSLDNDSIIVDKGLFYCCSDTIEVKAFTQKNFSSAL
                     LGGEGFFQIELSGTGVIVLECVVPQSEIVEYELKNGEELKVDGNFAIARTSGVNFSVT
                     KSDKSLIGSALNGEGFLNTFTGNGTVWLAPTQVMYEKLSFGAMPTNDNMNNKVSKQK"
     gene            2092..2556
                     /locus_tag="PC471_003601"
     CDS             2092..2556
                     /locus_tag="PC471_003601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDF2790684.1"
                     /translation="MNLNKVKFTEEHAIEVTNWKYEGKYSIYNLPPWEEIKKKNFSLA
                     KEDKRKNFISFINDNKELIGFINLLDEGSSVFFGIGMKPNYCGMGIGKEIIGLGLQEC
                     RNKYSTKPIVLNVRTWNMRAVKCYESQGFKIVETKVQKTHLGDGEFFVMRYQ"
     gene            complement(2823..3467)
                     /locus_tag="PC471_003602"
     CDS             complement(2823..3467)
                     /locus_tag="PC471_003602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2790685.1"
                     /translation="MFGQSFQNCLVLIKENFKLSTLTNLICSIILLCMVPLIQGVANL
                     DRISTAICLENYVAIIGIVMLVPVFSLEQSKEIDEIVSSKALSQVIVQLIRILMSIIG
                     IVVLIIGFVNVLIYLGCNFPVVEYIIGSFASAMFIGSLGMFFSNLFNNTIIGYMISIG
                     YLILNMMTKGKYVGKFFIMSMSQGSFDEKYWLILGSVVLIIASLLIKPVKRKIR"
     gene            complement(3397..4686)
                     /locus_tag="PC471_003603"
     CDS             complement(3397..4686)
                     /locus_tag="PC471_003603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2790686.1"
                     /translation="MNLFIKECKNILKSMVYYIFIVVLVFFYISQLGSSVSNDVSKNI
                     PNDGNPLVAPQKVENIEEYYSVHNQYPYGQKESMKTEKVIPNATINLIREYQKNTYTT
                     YPTGVVKYIKLDQNKTKTIEKYIEEITGKSISEINSLWKTDESIKIDYSKVSLERFEE
                     IMQRVDDILGGGSAYAFDKLQRFGVVPVTYDEAMKNYNYTIQKDKVSNGYARIFCDYI
                     GIVLSIFPVFVAVFMSLKDKRSRMREIIYSKQVSSCKIVLSRYFALVFMMILPVLILG
                     MKDLLPLIFFAKDKGISIDNLAFIKYTLWWILPTLMIVTAVGMSITILTDTPVAIIVQ
                     LIWWLYSINSTGLKGDYSTMGLMIRHNSVLNGDIIIQNFNQIMINRIVISLGALVLVL
                     LAIFLYEQKRRGNLDVWAKFSKLFSSNKRKLQIKHFN"
     gene            complement(4683..5555)
                     /locus_tag="PC471_003604"
     CDS             complement(4683..5555)
                     /locus_tag="PC471_003604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2790687.1"
                     /translation="MEMKIAVSNLSKRFGKKQALDNINLEIDSGMFGLLGRNGAGKTT
                     FMRILATLLSKTEGSIHMCGVPIENTKEIRNMVGYLPQDFSMYPNMTVYKAMDYLAVL
                     SDIPLRKRRKIIAKLLSKVNLTNYQNVKVKSLSGGMNRRLGIAQALIHNPRVLIVDEP
                     TAGLDPEERIRFRNLLCEIANDRIVILSTHIVGDIEATCENVAILNSGKVIYNGSTEA
                     LAKLAEGKVYSIEVDKKDIENIKSRFIVIGMLTHGGKAILRIISDDKPFETAVNCNPT
                     IEDGYMLIMGGDNI"
BASE COUNT         2032 a          776 c          698 g         2103 t
ORIGIN      
        1 ataattttaa catgcccata aaatataata cccacaaatg ataaataata ctcaaaatgt
       61 ctattgaaat aactatacat ccttaaataa cttaacttaa atctaaacat ttcaaaaact
      121 tcttaaaaat atgtaaaaat ttgatgataa agtagcgatt ttttcaaaaa aaatgtaata
      181 tagagttata tcttataata ttttagagat aaattttatc atagtgggga gaaatttgta
      241 tggatttaag caaaaaagta aaaattttag aaaatgcatc agatggcaaa agcgaatttg
      301 agatattaga atttgaaaaa cttgagggag cttctagccc attaatggca atgggattat
      361 attttgctag agaagctggt ttgaaagcta gacaaattaa gataaaatta aataattcat
      421 caataaaaac agaagctggt gcattatact attataaagg aaatatatct tctgaaacta
      481 agattggtgg tgttggaggt ctttttaaaa aaggaatatc aggagcactt actaatgaat
      541 caatgatgaa acctgtatac aaaggtactg gagaagttta tttggaacct tcttttagac
      601 attatttatt tatgagtctt gataatgatt ctataattgt tgataaagga ttattctatt
      661 gttgctctga tacaatagaa gtaaaagcat ttacacaaaa gaacttctca tctgctttac
      721 ttggtggtga aggcttcttc caaatagaat taagtggtac tggtgtaatc gttctggaat
      781 gtgttgtacc tcaaagtgaa attgtagagt atgaattgaa aaatggtgaa gaattaaagg
      841 tagatggtaa ctttgcaata gcaagaacat ctggtgttaa tttttctgtt acaaaatcag
      901 ataaatcttt aattggttct gctttaaatg gtgaaggttt cttaaataca tttactggta
      961 atggtacagt ttggctagct ccaactcaag taatgtatga aaaactaagt tttggagcaa
     1021 tgcctactaa tgataatatg aataataaag tttctaagca aaaatagttg ttttataaat
     1081 atttattaaa tcattattga ttttataaag tcactataaa ttttattaga ttattattat
     1141 aaaatcagag tgtggaaatg tttatgtgta ttctttaaat aaaaatttcc acattctgta
     1201 aggttattat acttgatata taaaattcaa tttaggaaca tagtctaaca tttataaaca
     1261 tattaaggta atgatttctc ttggtaatta ttctcatatc ttattctttg attatattta
     1321 tcttattcct agcccttatg tcatattata tttcttatct tattacttat aattagcatt
     1381 aacttatcta aaatcttgta tttttttaat agtaaagttt acatctatat taaaaagttt
     1441 acatctatat taatttcata aaacaatcaa ggtagtcaag agattcaact ttacttgatt
     1501 ttaaaaattt aagtgaattt gttcttatac acatcttttt aaaattatat ctgtagaaaa
     1561 tatcatttag agctataatt aaattaactt attaaatttt agaaaattta ttaaaaaata
     1621 taagttaaat taatattttt aaagattctt ttttgtaact actagaatag taatttagta
     1681 gttattgtat ctctaaagtt taagcatgga tagatataca tttgataatt tgcctataat
     1741 ttacctacta cttggattat tggagcaaat acaatatatt aataataagt tcctaaaacc
     1801 aataaattaa ctaatacaca taaagatatt gatgttgatt atacaactat aagaaacgga
     1861 tttaacagaa tttaatcaag ctaagaaatt atatatatat cgtgatgttt ttgaattaga
     1921 aggtaattca aaattagttt atagtcttct gtcaatccaa taaaaagtac agggtacaag
     1981 aattgttagt aaaattttta aagattttat ggataaatat aaatagtttt acttattttc
     2041 taaaacttta aaataccatt aattacctta aatattttaa taggaggaca tatgaattta
     2101 aataaagtta aatttactga agaacatgct attgaagtta ctaattggaa atatgaaggt
     2161 aaatattcaa tatacaatct acctccatgg gaggaaatta aaaagaagaa tttttcacta
     2221 gcaaaagaag ataaaagaaa aaattttata agttttataa atgacaataa agaattgata
     2281 ggttttataa acttattaga tgaaggaagc tctgtattct ttggtattgg tatgaaaccg
     2341 aattattgtg gaatgggaat tggtaaagaa ataattggcc taggattaca agaatgtaga
     2401 aataaatata gtactaaacc aattgtatta aatgtaagaa cctggaacat gagagctgta
     2461 aaatgttatg agtctcaagg atttaaaata gttgagacta aggtacaaaa aacgcattta
     2521 ggagatggag aattttttgt tatgaggtat caatagttta taacaatagt cttattgtta
     2581 atacaacaat taaaatgaaa aaatacatat gtgtaggtca tagcaataag tgtctttgaa
     2641 atataaattt gagagctttt gataaaattt tgtatacata tatattttat actacggata
     2701 tataaatcta tctacatata taaagagatt gtaagtaggt ttgtgtatat aaaaatagat
     2761 ttatatttca taaatgtact ttttatttgt attaccttta ttttatctaa gctctcattt
     2821 tattatctaa tcttcctttt tactggtttt attaagagag atgcaattat caacacaaca
     2881 cttccaagta tcagccaata tttctcatca aaactacctt gactcataga cattataaaa
     2941 aattttccaa catacttgcc tttagtcatc atatttaaaa ttaaatatcc tatagatatc
     3001 atataaccta tgattgtatt attaaataaa tttgagaaaa acattcctaa acttcctata
     3061 aacatagctg atgcgaaact tccaataata tactcaacta ctggaaaatt acatccaaga
     3121 tatattaaaa catttacaaa acctatgatt aataccacta tacctataat tgacatcagt
     3181 atcctaatta attgtacaat aacttgagat aatgcttttg aagatactat ctcatcaatt
     3241 tcttttgatt gctctagcga aaatacaggt accaacatta ctatccctat tattgcaaca
     3301 taattttcta aacatatagc tgttgaaatt ctgtctaaat tagctactcc ttgtattaga
     3361 ggaaccatac atagcaatat aattgaacat attaaattag ttaaagtgct taatttgaag
     3421 ttttctttta ttagaactaa acagttttga aaactttgcc caaacatcaa gattccccct
     3481 tcttttttgc tcatataaga atatagctaa caaaacaagc accaatgctc ccaagcttat
     3541 cacaattcta ttaatcataa tttgattgaa gttttgtata ataatatccc catttaatac
     3601 actattatgt cttatcatta gccccattgt tgaataatca cctttgagtc ctgtagaatt
     3661 tatactgtaa agccaccaaa ttaattgcac aataattgct actggtgtat ctgttaatat
     3721 agtaatactc attcctacag ctgtaactat cataagagtt ggtagtatcc accataatgt
     3781 atattttata aatgctaagt tatcaataga tataccttta tctttagcaa agaaaatcaa
     3841 aggaagtaaa tctttcatac ctaaaattaa tacaggcaat atcatcataa agacaagtgc
     3901 aaaatatctt gataaaacta ttttgcatga agatacttgt tttgaataaa taatctctct
     3961 cattcttgaa cgcttatctt taagtgacat aaaaactgcc acaaatactg gaaatatact
     4021 taaaactata cctatataat cgcaaaaaat tcttgcatac ccattactaa ctttatcttt
     4081 ttgtattgta tagttgtaat tcttcatagc ttcatcatag gtaactggaa ctactccaaa
     4141 tctttgtaac ttgtcaaaag catatgcaga ccctcctcct aaaatgtcgt ctactctttg
     4201 cattatttct tcaaatcttt caagagatac tttagaataa tctattttta tactttcatc
     4261 agttttccaa agtgaattta tttcgctaat acttttacca gttatttctt ctatatattt
     4321 ttctatggtc ttcgttttat tttggtctaa ttttatatac ttaactaccc ctgttggata
     4381 cgttgtatat gtgttttttt gatattctct aattaggttt atagttgcat ttggaatgac
     4441 cttttctgtt ttcatagatt ctttttgacc atatggatat tgattgtgta cagaataata
     4501 ttcctcaata ttctctactt tttgtggagc aactaatgga tttccatcat taggaatatt
     4561 tttacttaca tcatttgaaa ctgaacttcc aagttgagaa atatagaaaa aaactaaaac
     4621 tacaataaaa atataatata ccatactctt taatatattt ttacattctt ttataaatag
     4681 attcatatat tatctcctcc cattataagc atatagccat cttcaatagt tggattacaa
     4741 tttactgctg tctcaaaagg tttatcatct gatataatcc taagtatagc ctttccacca
     4801 tgagtcaaca ttcctataac tataaatcta ctttttatat tttcaatgtc ttttttatct
     4861 acttcaatac tatatacttt cccttcagct agttttgcta atgcttctgt tgagccattg
     4921 tatatgactt ttccactatt caatatagct acgttttcac atgtagcttc tatatctcca
     4981 acaatatgtg tagataaaat aacgattcta tcatttgcaa tttcgcatag taaatttcta
     5041 aatcttatac gttcttctgg gtcaagacct gctgtcggct catctactat taaaactctg
     5101 ggattatgaa ttaatgcttg tgctatccca agccttctat tcatacctcc agataaagat
     5161 tttactttca cattttgata attagtaagg tttactttgc ttaataactt tgctataatt
     5221 tttcttcttt ttcttagtgg tatatccgat aaaactgcta aataatccat tgctttatat
     5281 actgtcatgt taggatacat tgaaaaatct tgtggcaaat aaccaaccat atttctaatt
     5341 tccttagtat tctcaatagg aactccacac atatgtattg aaccttctgt ttttgataat
     5401 aaagtggcta aaattctcat aaatgtggtt tttcctgctc catttcttcc taaaagacca
     5461 aacataccac tgtcgatttc aagatttata ttatcaagag cttgtttttt cccaaatctt
     5521 ttactaagat tggatactgc tattttcatt tccatatata aatacacccc cagaaacaat
     5581 tttatagaat tttattttta taaattttt
//
LOCUS       DAMJYR010000200         5605 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.205, whole genome shotgun sequence.
ACCESSION   DAMJYR010000200 DAMJYR010000000
VERSION     DAMJYR010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5605)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5605)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5605
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.205"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(47..208)
                     /locus_tag="PC471_003605"
     CDS             complement(47..208)
                     /locus_tag="PC471_003605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2790688.1"
                     /translation="MNNFLLNVIAGVIASLIFCLICKVFLKVKSHSTRGKSKSGWEFD
                     FKIKFHKFK"
     gene            complement(549..989)
                     /locus_tag="PC471_003606"
     CDS             complement(549..989)
                     /locus_tag="PC471_003606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HDF2790689.1"
                     /translation="MSNLSAFLSQNAIKVDNVKYVASNRFLDKEGKPVEWELKVLSSE
                     EDEALRRKCTKRVKVIGNNGKHTGQYTSEIDYNSYVAELCVASTVFPDLKDAELQNSY
                     GVMGEAQLLKTMLTAGEYVNYTVKVNEVNGFDTSFEDKVEEAKN"
     gene            complement(1061..1531)
                     /locus_tag="PC471_003607"
     CDS             complement(1061..1531)
                     /locus_tag="PC471_003607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HDF2790690.1"
                     /translation="MAQTINAKDTVSAKKAECFITIEGKRYNFMQAIDLEAKMEKNKS
                     EVPILGRTTKGNKTTGSTNTGSATFHYNTSIFRELLYRYKETGEDIYFDIQVTNEDPT
                     SAVGRQTVVLKDCNMDSGIITKFDADGEYLDEDMDFTFEDWELVEKFNLLAGME"
     gene            complement(1548..2858)
                     /locus_tag="PC471_003608"
     CDS             complement(1548..2858)
                     /locus_tag="PC471_003608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath family protein"
                     /protein_id="HDF2790691.1"
                     /translation="MALGGGTFVTQNKVLPGAYINFVSATRATSSLSDRGIVAIPLEL
                     DWGIDKDVFQVTSDDFEKYSVKYFGYDYTHEKLKGLRDLFKNIRLGYFYKLNKGVKAS
                     CTIATAKYSGIRGNDLKVTVTTNIDDNTKFDVVTLLDNKKVDTQIAKVITDLQDNDYI
                     TWKKDATLEASAGLVFTGGTNGEAVTGAEYQAFLDKIESYSFNALGCLATTTEIKSLF
                     VEFTKRMRDKVGAKFQTVLYKKSDADYEGVVSVENKIKDKDLVESSLIYWAAGAIAGC
                     DINKSNTNKKYDGEFDVDVNYTQIQLEEALKTGKFIFHKVGDEVHVLEDINTFVSFTD
                     DKNDDFSSNQSVRVLDQIANDIATLFNEKYLGKVPNDKAGRISFWNDVVKHHKELENI
                     RAIEDFKTDDVSVELGNDKKTVIVSDAVKVINAMSKLYMTVSVS"
     gene            complement(2859..3035)
                     /locus_tag="PC471_003609"
     CDS             complement(2859..3035)
                     /locus_tag="PC471_003609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2790692.1"
                     /translation="MSKTLSKEDNYKFTKEQIVNSKKYVNRKDLLNAILKENDLYSFS
                     EVEDRINKFMKGVS"
     gene            complement(3028..3465)
                     /locus_tag="PC471_003610"
     CDS             complement(3028..3465)
                     /locus_tag="PC471_003610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2790693.1"
                     /translation="MLNNIIDGISIKLDKTFGESYTIYSEDVEQGINEPCFFIVPLNP
                     SKVSYLSGRTLKKNSFDVHYFPKSNDKSFEINEIAEMLLEELEYIEIDGDLVRGTNMN
                     FEIIDNVLHFFVDYNYFTIKSNDTDKMDTVELFGGLKRGDNFE"
     gene            complement(3458..3883)
                     /locus_tag="PC471_003611"
     CDS             complement(3458..3883)
                     /locus_tag="PC471_003611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HDF2790694.1"
                     /translation="MARWGSVDFREFKRVCKKMEELTKIDLDKFCKDAARELAARLLG
                     KVIRRTPVDTGFLRQGWNGVAYARSLPVYKQGNNYIIEVVNPTTYASYVEYGHRTKDG
                     KGWVKGQHFLTISEMELQSQVDKIIEKKLLILLKGVFDA"
     gene            complement(3883..4230)
                     /locus_tag="PC471_003612"
     CDS             complement(3883..4230)
                     /locus_tag="PC471_003612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015984843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2790695.1"
                     /translation="MVSKTRKAIEMLYRDKCTIVEYQPIKDPVTKRTNNKEVIVLENQ
                     PCKLSCKNIVSATEGKVAKLEQTIKLFISPDIEIKAGSKLIINDKEYVRSGESAIYPN
                     HQEIILELFKDKA"
     gene            complement(4224..4625)
                     /locus_tag="PC471_003613"
     CDS             complement(4224..4625)
                     /locus_tag="PC471_003613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2790696.1"
                     /translation="MGNNIIDDIEKRLESFGYILKDGDKWLIDFVREKIENIIKLDCN
                     IKTMPIELKEIEVDMIVGEFLFTKKNMGQLDIESINFEAVEKSISEGDTKVDFAIGSG
                     SQTPEQRFDSLVAYLTTYGKNKILTFRCLRW"
     gene            complement(4635..4868)
                     /locus_tag="PC471_003614"
     CDS             complement(4635..4868)
                     /locus_tag="PC471_003614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2790697.1"
                     /translation="MAQVRKLNRILTIEECKIDDFLEMGYDLIDETGKAAKYGKSLNV
                     KDLIAENNILRSKVESLEEENKQLKEKNKLTKK"
     gene            complement(4868..>5605)
                     /locus_tag="PC471_003615"
     CDS             complement(4868..>5605)
                     /locus_tag="PC471_003615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsid protein"
                     /protein_id="HDF2790698.1"
                     /translation="VDSNRDTIAVAQRNYDNAWEPKVLTNQRKWSTLVHPADINQTNY
                     VASIGNITKVYNEEQKFPEMDAYCISKIYADWTALGNTADTTVLTTTNVLEVFDKLME
                     KMTEARVPENGRILYVTPVVNTLIKNAKEIQRTVNIKDAGTSLNRQTTDIDTVKIIKV
                     PSNLMKTAYDFTTGWKVGAGAKQIFMSLVHPSAIITPVSYQFSKLDEPTAVTEGKYFY
                     FEESFEDVFILNKKADAIQFVVEGAGA"
BASE COUNT         1641 a         1125 c          582 g         2257 t
ORIGIN      
        1 actacaatct attttgcggt agagtgaagt tcataattaa atgaatctat ttgaacttat
       61 ggaacttgat tttaaaatca aattcccagc cacttttact cttgccacga gttgagtggc
      121 tttttacttt tagaaatact ttacaaatta agcaaaatat taaactagca ataacgccag
      181 ctattacatt aagtaaaaag ttattcatac ttcccacctc ctttcattag gaagtaggtt
      241 ttatctcagt atgaactcca ctctataaat tgtagattac atcttcttgc taaaaatatt
      301 ataacatata attattacat attttaccta ttctatattt atttttttat tttgctatct
      361 tcttcgtccc ctctttctct ctctttcagc ttctttcatt gcttcctctt catcttctat
      421 ctttataagt attgaggcgg ctgctaacgc tctctcatta acttctaaat tcatatattc
      481 acttggcttc cactttaatt tttgaataca ataatgagtg atgctagcat caaaatcgcc
      541 acctctgatt agttttttgc ttcttctact ttatcctcaa aagatgtatc aaatccattg
      601 acttcattca cttttactgt ataattgaca tactcacctg ctgtaagcat tgtctttaat
      661 aactgagctt ctcccattac tccataacta ttttggagtt cggcatcctt taaatctgga
      721 aatactgtag atgctacaca taattcagct acataactat tgtagtcaat ttcacttgta
      781 tattgtccag tatgcttacc attgttacca atcactttta ctcttttagt acactttctt
      841 cttagtgctt cgtcttcttc agatgataaa acttttaatt cccattcaac tggtttccct
      901 tctttatcta aaaatctgtt actcgctaca tattttacat tatcaacctt tattgcattt
      961 tgacttaaaa aagcacttaa attactcata ttattctaat ctccttttat tttaattttt
     1021 catataaaaa atacacatat ataatttata aatgtgtatt ttactccatt cctgccaata
     1081 aattaaattt ttctactaat tcccaatcct caaaagtgaa atccatatct tcatctaaat
     1141 actcaccatc agcatcaaat ttagtaatta ttccactgtc catattacaa tctttaagta
     1201 ctactgtctg tcttcctaca gcagatgtag ggtcttcatt tgtaacttgt atgtcaaaat
     1261 aaatatcctc accagtttct ttatatctgt aaagtaattc tctaaaaata gaagtattat
     1321 aatgaaatgt tgcacttcca gtatttgtac tcccagttgt tttatttccc tttgttgttc
     1381 ttcctagaat tggaacttca cttttatttt tttccatttt agcctctaaa tctatagctt
     1441 gcataaaatt atatcttttg ccttctatag ttataaaaca ttcagctttc tttgcactaa
     1501 ctgtatcttt agcatttatt gtttgagcca tattatcact cccctctcta actaactgaa
     1561 actgtcatat aaagcttact catagcattt ataaccttaa cagcatcaga tactatgaca
     1621 gttttcttat catttccaag ctctacacta acatcatcag ttttaaaatc ttctattgcc
     1681 cttatattct ctaattcttt atggtgttta acaacatcat tccagaaact tattcttcct
     1741 gccttatcat tcggaacttt acctaaatac ttttcattaa ataaagttgc aatatcatta
     1801 gcaatttggt caagtactct aacactttgg ttacttgaaa aatcgtcatt tttatcatct
     1861 gtaaatgata caaaagtatt tatatcctct aacacatgaa cttcatctcc caccttgtgg
     1921 aatataaatt taccagtttt taaagcttct tcaagttgta tttgtgtata attaacatca
     1981 acatcaaact caccatcata ctttttatta gtattagatt tatttatatc gcatcctgct
     2041 atagctccag ccgcccaata aattaaacta gattcaacta aatctttatc tttaatctta
     2101 ttttctacag acactacacc ttcataatct gcatcacttt tcttatatag tactgtttga
     2161 aacttagctc ctaccttatc tctcattctc tttgtaaatt ctacaaataa acttttaatt
     2221 tctgttgttg tagccaaaca tcctaaagca ttaaagctat agctttctat tttatccaag
     2281 aaagcttggt actctgctcc tgtcacagct tcgccattag ttccaccagt aaatacaagt
     2341 cctgcacttg cttctagtgt tgcatccttc ttccaagtga tatagtcatt gtcttgtaag
     2401 tctgtaataa cctttgctat ttgagtatct accttcttat tatctaaaag tgttacaaca
     2461 tcaaatttag tgttatcatc tatatttgtt gtaactgtta cttttaagtc atttcctctg
     2521 ataccactat attttgctgt ggctatagta caactggctt taacgccttt atttaattta
     2581 taaaaatatc ccaaccttat atttttgaat aaatctctca aacctttcag cttctcatga
     2641 gtataatcat atccaaaata cttcactgaa tacttctcaa aatcatcact ggttacttga
     2701 aatacgtctt tatctatgcc ccaatctaac tctaaaggta ttgcaacaat acctctatcc
     2761 gataatgaac tggttgccct tgtagcactt acaaaattta tatatgcacc aggtaggacc
     2821 ttattttgtg ttacaaatgt tcctccacct aaagccatct aactcactcc tttcataaat
     2881 ttatttattc tatcctctac ctctgagaag gaatataaat cattttcttt taaaattgca
     2941 tttaataaat cttttctatt tacatacttc ttagagttaa ctatctgctc cttagtaaac
     3001 ttgtagttat cttctttgct taatgtttta ctcaaaatta tcacctctct tcaaaccacc
     3061 gaataactct actgtatcca tcttatctgt gtcattactt tttatagtaa agtagttata
     3121 atcaacaaag aaatgaagaa cattatctat aatttcaaag ttcatatttg tacctctgac
     3181 taagtctcca tcaatttcta tatactctaa ttcctccagt aacatctcag ctatctcatt
     3241 tatttcaaat gatttatcat tacttttagg aaaataatgt acatcaaaag agttcttttt
     3301 taatgtcctg ccacttagat aggatacttt gcttggattt aaaggaacaa taaaaaaaca
     3361 aggttcattt ataccttgct ccacatcttc actataaatt gtataactct ctccaaatgt
     3421 tttatctaat ttaatagata ttccatcaat tatattatta agcatcaaat actcctttaa
     3481 gcaatattaa tagttttttc tctataatct tatcaacttg gctttgtagt tccatctctg
     3541 aaattgttaa gaaatgttgt cctttaaccc aaccttttcc atctttagtt ctatggccat
     3601 attcaacata acttgcatat gtggtcggat taacaacctc tataatataa ttatttcctt
     3661 gtttatacac aggaagcgac ctagcataag ccactccatt ccatccttgt cgtaagaatc
     3721 ctgtatcaac tggtgttctt ctaattactt tcccaagtaa tcgtgctgct aattctcttg
     3781 ctgcatcctt gcaaaactta tctaaatcaa tctttgtaag ctcctccatc tttttacaaa
     3841 ctcttttaaa ctctctaaaa tcaacactgc cccatctagc cattatgctt tatccttaaa
     3901 taactcaagt attatttctt gatgatttgg atatatagct gattctccac ttcttacata
     3961 ctctttatca tttataataa gttttgaacc tgctttaatt tctatatctg gagatataaa
     4021 gagtttaata gtttgctcta gcttagctac tttcccttct gtagcagaaa ctatattttt
     4081 acatgaaagt ttacatggtt gattttctaa tacaatcact tctttattgt tagttcgttt
     4141 tgttacaggg tctttaattg gctgatactc aactatagta catttatctc tatataacat
     4201 ttctattgct tttctagttt tacttaccat cttaagcacc taaaggttaa tatcttattc
     4261 ttaccataag tagtaagata agctactaag ctatcaaagc gttgttctgg tgtttgagag
     4321 ccacttccta tagcaaaatc tacctttgta tcaccttctg atatagactt ttctacagct
     4381 tcaaagttaa tgctttctat atctaattgc cccatatttt tcttggtaaa taagaactct
     4441 ccaactatca tatcaacttc aatttctttc aattcaattg gcatagtttt tatattacaa
     4501 tctagtttaa taatattttc tattttttct cttacaaaat ctattaacca cttatcccca
     4561 tcttttaata tatatccaaa actttcaagt cttttttcta tatcatcaat tatattattt
     4621 cccataattt tcacctactt tttagtaagt ttatttttct ctttaagctg tttattttct
     4681 tcttctaaag actcaacttt tgaccttaaa atattatttt cagctattaa atcttttaca
     4741 tttaatgact tgccatactt tgctgcctta ccagtttcat ctatcaaatc atatcccatc
     4801 tctaagaaat catctatttt acattcttct atagttaata ttctatttaa tttccttact
     4861 tgtgccatta tgctccagct ccttcaacaa caaattgtat tgcatcagct tttttattta
     4921 atataaatac atcctcaaaa ctttcttcaa agtagaagta ttttccctct gtaactgctg
     4981 ttggttcgtc taacttagag aactgataag aaacaggtgt aattattgca cttgggtgaa
     5041 ctaaggacat aaagatttgt ttagctcctg ctcctacttt ccatccagtt gtaaaatcat
     5101 atgcagtttt cattagatta gatggtactt taattatttt aactgtgtca atatcagttg
     5161 tttgacgatt aagagaagtt cctgcatcct ttatatttac tgttctttgt atctcttttg
     5221 catttttgat aagtgtattt actactggag taacatacaa tattcttcca ttttcaggta
     5281 ctctagcttc tgtcattttt tccattaact tatcaaatac ttctaatacg tttgttgttg
     5341 taagaacagt tgtatctgct gtgttaccta atgcggtcca atcagcatat attttagata
     5401 tacagtaagc atccatctct ggaaactttt gttcctcatt atatactttt gttatattgc
     5461 ctattgaagc cacataatta gtttggttta tatctgctgg atgaaccaat gttgaccatt
     5521 tcctttgatt agttaatacc ttaggttccc aagcattatc atagtttctt tgagctactg
     5581 ctattgtatc tctgtttgaa tctac
//
LOCUS       DAMJYR010000201         5603 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.206, whole genome shotgun sequence.
ACCESSION   DAMJYR010000201 DAMJYR010000000
VERSION     DAMJYR010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5603)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5603
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.206"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            132..632
                     /locus_tag="PC471_003616"
     CDS             132..632
                     /locus_tag="PC471_003616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HDF2790699.1"
                     /translation="MRKNSINIALTAAITAIYAVLTLSLGFISYGPIQFRVAEIMMLL
                     AFLDKGYIVGLTLGCFLANVIGPYGVPDIIFGTLATFISASMIYITRKLGKQNKSTLV
                     IASLWPTVINSIVVGLMLNVFFGLPLILSMIQVGFGEFVVVTIVGVPFFCFLMKKYKN
                     VINIKF"
     gene            689..1675
                     /locus_tag="PC471_003617"
     CDS             689..1675
                     /locus_tag="PC471_003617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HDF2790700.1"
                     /translation="MSKVKRLTDVWIYINDKQKFTIKELSEEFNLSAKTIQRYLKELN
                     KMGLPIQAEQGRNGGYRVLNNSYIPPVIFTEKEVMIILFALKSLQIYNYKLMEIEINS
                     IMRKVSYERKSSIKVGIENIKRYIGFVANDTNDGDFKSSNVTEFFRASSESLILNIKY
                     KCGNQILEKNVCPIGIYLNKGAWFSPAYDCKSDKIILVHIDNIIDIKRTGESKKIHIN
                     LEEWLHEVYNNLYGNSSTTKKFGEIIYLNVLLTKEGVSKIKDSSWNLDMELNEDGSGI
                     ISTFIKVEDIDCYASILFFIGNNAKVIEPKFLNKLLYDKANELRYFYEENMV"
     gene            1725..2447
                     /locus_tag="PC471_003618"
     CDS             1725..2447
                     /locus_tag="PC471_003618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein"
                     /protein_id="HDF2790701.1"
                     /translation="MENFNKNAKIIGIGAEGINIINEVEEKIKANMDIEKININQEIE
                     KEYVRSLLDGVDILFLIYSSEDKHIRDIIKAVSYMSNERRVLSIGMDCSEKENKEDLE
                     LGREFKINNDSIFKFVDLMNIMVESISDSCMINIDITDLKEAIVGDKGIKYSFEEFED
                     TKSYSEIADILFDRMEYIGEEFISKKGIVFVEGSPEFSIMELNDLISNIQSKVEESYE
                     VIFSLYIKENLNGNIRVGLLYN"
     gene            2476..4410
                     /locus_tag="PC471_003619"
     CDS             2476..4410
                     /locus_tag="PC471_003619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDF2790702.1"
                     /translation="MSENIRGTEYWLMEEFFYQETDTPQIFREESIIALGFDIKINLK
                     KVFSMGKKSEKYINENCNDKDFIKKFMSIKKGDYFLYKAYQNIEEKVKCNVSIGIVEE
                     DFENGYELSHILGHTLPVKWMHNFDTVSNCKVYTSEFYKLNNNDISSYLEIIKSQDKK
                     LLRKRIEESENNKWYLKNYYYKNVDKCVDDEELFIQPINLEQLESLKNIKTDDYILLY
                     NSEKVICENDHTMKSLFGVCQVMEGFDKEKVSALEGGYFFPVRWIEYEKENIDGNAWV
                     IFENNSIVEKYVKRFTNKMPLNTILYGPPGTGKTYNTKQMVYNLVSETSLRNRYYTGY
                     LDYKNNVEFCTFHQSYGYEEFIEGLKSDGEGNFKPEDGILKDISIEACYNALKFEKKM
                     SFELEKDSLTLETIKDRKKKLVLENIEYSESFNFEESENYVLIIDEINRGNVSKIFGE
                     LITLLEDDKRLTKSNQTIVKLPYSKEKFSLPPNLYIVGTMNTSDKSIALLDIALRRRF
                     NFEEIMPDYDLLSIVDGIDIRKMLYTINKRIEFLYDRDHVIGQAYLLAVENINDIVVV
                     FRSKIIPLLQEYFYYDSEKVGLILGGIGRNEKDKYIVYKEKLIASELFKNNNASIMES
                     KVNYCIKQNISSEELKNIYE"
     gene            4403..>5603
                     /locus_tag="PC471_003620"
     CDS             4403..>5603
                     /locus_tag="PC471_003620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="McrC family protein"
                     /protein_id="HDF2790703.1"
                     /translation="MSKNIYVKETYEWIRIGNGENELTEIEYEKLLKYLENNNDVLKS
                     NIIDIKYKKLRFINYVGIICFENVILEILPKLSLSDNLVKDREILLQMLSICNKIPIT
                     MNEKIRLSLKNYNLLNFFVMYFIESMQTQMKKGIYFEYINKIENLNVMRGKILLSTYA
                     KEKGISPMKIRCEYDEYSENNFLNQVLKKACISILCRINDNSIQGKIKKILSYFQNVD
                     LIYIDRKKLLDYKFYKNNDRFKDCYLLARLLLLNLSMDNSQNNQEAFSILFEINTLYE
                     EYIGILIKSIWDNSFRETYIQDKSKFLLKNEQTGKKNFNLRPDIVLYDLKNEYEIIID
                     TKWKAIEVDSNVFYRSSDIYQMYAYITAYENAKRCILLYPCIQKDKNYSSWKLSESFK
                     GKFIEAKT"
BASE COUNT         2340 a          428 c          965 g         1870 t
ORIGIN      
        1 aatgaattgg gaaaatagtt cgctaaaata atttgttttt ataaaattac tgtttaagtt
       61 tatagatttt gtataaaata aagttatgtt attaattttt ttaaaaataa aacttttact
      121 aggagacaga tatgagaaaa aatagtatta atatagcact aactgcagca attacagcaa
      181 tctatgcagt tttaacatta tcattgggat ttataagtta tggaccaata caatttaggg
      241 tagcagagat tatgatgctt ttggcatttt tagataaagg atatatagta ggtcttacat
      301 taggttgttt tttagcaaat gtaataggac catatggtgt acctgatata atttttggaa
      361 cattagccac atttataagt gcaagtatga tttatattac tagaaagtta ggtaaacaaa
      421 ataaaagtac tttagttata gcttctttat ggccaactgt tataaattcg attgtggttg
      481 gtctaatgtt aaatgtattc tttgggcttc cattaattct atctatgata caggtaggtt
      541 ttggagaatt tgtagtagta acaattgtag gagtaccatt tttctgcttt ttaatgaaaa
      601 aatataaaaa tgtcattaat attaaatttt aaatagtgta tagtaattta attaattgag
      661 agtattatta gtgaaaagag gtgtttattt gtcgaaggta aaaagattga ctgatgtttg
      721 gatatatatt aatgataagc aaaagtttac aataaaagag ttgtcagagg aatttaatct
      781 ttcagctaaa acaatacaac gatatttaaa agaattaaat aaaatgggac ttccaattca
      841 agcagaacaa ggtagaaatg gaggttatag ggtgctaaat aatagctata tacctcctgt
      901 tatatttaca gaaaaagaag ttatgattat actatttgca cttaagtcac ttcagattta
      961 taattataag ttaatggaaa tagaaattaa ttctataatg agaaaagtaa gttatgaacg
     1021 taaaagcagt ataaaagtag gtatagaaaa tattaaaaga tacattggct ttgtagctaa
     1081 tgatactaat gatggagatt ttaagagtag taatgtaact gagtttttta gagcttcttc
     1141 agaatctcta attttaaata ttaagtataa gtgtggtaat cagattttgg aaaaaaacgt
     1201 atgccctata ggtatatatt taaataaggg agcatggttt tcaccagcat atgattgtaa
     1261 aagtgataaa ataatattag ttcatataga taatataatt gatataaaaa gaacaggaga
     1321 aagtaaaaaa atacatataa acttggaaga atggttacat gaagtatata acaatttata
     1381 tggaaatagt tcaactacta aaaaatttgg tgaaattatt tatctaaatg tattattaac
     1441 taaagaaggt gtttctaaaa taaaggacag ttcttggaat ttagatatgg agctaaatga
     1501 agatggtagt ggaataatta gtacattcat aaaggttgaa gatatagatt gttatgcttc
     1561 gattttattt tttattggaa ataatgctaa ggttatagag ccaaaatttt tgaataagct
     1621 actttatgat aaggccaatg agttaagata tttttatgaa gaaaatatgg tataatattt
     1681 agaaatgttg aaattaaata attattaagg agatatacgt tataatggaa aattttaata
     1741 agaatgctaa aataattggt attggagctg aaggtattaa tataataaat gaagtagaag
     1801 aaaaaataaa agcaaatatg gatatagaaa aaataaacat aaatcaagag attgaaaaag
     1861 aatatgttag aagtctttta gatggtgtag atatattatt tttgatatat tcatctgaag
     1921 ataaacatat tagagatatt ataaaagctg tttcatatat gtcaaacgaa agaagagttc
     1981 tatctatagg aatggattgt tcagagaagg aaaataaaga agatttagag cttgggagag
     2041 agtttaaaat caataatgat agtatattta aatttgttga tttaatgaat atcatggtag
     2101 agtctatttc tgattcttgc atgataaata tagatattac tgatttaaaa gaagctattg
     2161 taggagataa aggaattaag tattcttttg aagagtttga agatacaaag tcatatagcg
     2221 agatagctga cattttattt gacaggatgg aatatattgg tgaggaattt ataagtaaga
     2281 aaggaattgt atttgttgaa ggaagtccag aattttctat aatggaattg aatgatttaa
     2341 taagtaatat tcagagcaag gtagaagaaa gctatgaagt aatattttct ttatatataa
     2401 aggaaaattt aaatggaaat attagagtag gcttattgta taattagtat attttagata
     2461 ttaaggagag tgtatatgtc tgaaaatata agaggtacag agtattggtt aatggaagaa
     2521 tttttttatc aggaaacaga cacaccacag atttttagag aagaaagcat aattgcactt
     2581 ggatttgata taaaaataaa tttaaaaaaa gtattcagta tggggaaaaa atcagaaaag
     2641 tatattaatg agaattgtaa tgataaagat tttataaaaa agtttatgtc gattaaaaaa
     2701 ggagattatt ttttatataa agcatatcaa aatatagaag aaaaagttaa atgtaatgtg
     2761 tctattggca tagttgaaga agattttgaa aatggatatg aattaagtca tattttaggg
     2821 catacactac cagtaaaatg gatgcataat tttgatacag tttcaaattg taaagtatat
     2881 acaagtgaat tttataaatt aaataataat gatattagtt catatttaga aattattaag
     2941 tcacaagata agaaattatt gagaaaaaga atagaagaaa gtgaaaataa taaatggtat
     3001 ttaaaaaact attattataa aaatgtagat aagtgtgtag atgatgaaga attatttatt
     3061 cagcccatta atctagaaca attagaaagc ctaaaaaata taaagacaga tgattatatt
     3121 ttattgtata atagcgagaa agtaatttgt gaaaatgacc atacaatgaa gagtctattt
     3181 ggcgtatgtc aagttatgga agggttcgat aaagagaaag taagtgcttt ggagggtgga
     3241 tatttttttc ctgttagatg gatagagtat gaaaaggaga atatagacgg aaatgcatgg
     3301 gttatatttg aaaataatag tatagttgaa aagtatgtaa aaagatttac taataaaatg
     3361 ccattaaata ctatacttta tggaccacca ggaactggta aaacatacaa tacaaaacag
     3421 atggtttaca atctagtaag tgaaaccagt ctaagaaaca gatactatac aggttactta
     3481 gattataaaa ataatgtaga attttgtaca tttcatcaat catatggata tgaggaattt
     3541 atagagggcc ttaagtctga tggtgaaggc aattttaagc cagaagatgg gatattaaag
     3601 gatatatcta tagaagcatg ttataatgca ttgaaatttg aaaaaaagat gagctttgag
     3661 ttggaaaaag atagcttgac tttagaaact ataaaagaca ggaaaaagaa attagtatta
     3721 gaaaatatag aatatagtga gagttttaat tttgaagaat cagaaaacta tgttttaata
     3781 atagatgaaa ttaatagagg gaatgtatct aaaatatttg gtgagctaat aacattattg
     3841 gaagatgata aaagacttac taaatcaaat caaactattg tcaaactacc atattcaaag
     3901 gaaaaatttt ctctacctcc aaatttgtat atagttggaa ctatgaacac atcagataaa
     3961 tcaatagcac ttttagatat agctttaaga agaaggttta attttgaaga aattatgcca
     4021 gattatgatt tattgtctat tgtagatggt atagatatta gaaaaatgct atatacaatc
     4081 aataaaagga tagagttttt atatgataga gaccatgtta ttggacaagc ttatctactt
     4141 gctgtagaaa atattaatga tatagtagta gtatttagaa gtaaaataat accacttctt
     4201 caagagtatt tttattatga ttcagaaaaa gtaggtttga ttttaggagg aattggaaga
     4261 aatgaaaaag ataaatacat tgtttataaa gaaaaactta tagcttctga gttatttaaa
     4321 aataataatg cctcaataat ggagagtaaa gttaactatt gtataaaaca aaacatatct
     4381 agtgaggagc taaaaaatat atatgagtaa aaatatatat gttaaggaaa cctatgaatg
     4441 gataaggata ggtaatggtg aaaatgagct tacagaaatt gaatatgaaa aattgcttaa
     4501 gtatctagag aacaataatg atgtattaaa aagtaatata atagatatta aatacaaaaa
     4561 acttagattt ataaactatg taggcatcat atgttttgaa aatgtaatac ttgaaatact
     4621 tcctaaattg tcattaagtg ataatcttgt aaaagataga gagatactat tacagatgct
     4681 ttctatatgt aataaaattc caattacaat gaatgaaaaa ataagattga gtttgaaaaa
     4741 ttataatctc ttaaattttt ttgttatgta tttcattgag agtatgcaaa cacagatgaa
     4801 aaaaggtatt tattttgaat atataaataa aatagagaac ttaaatgtta tgaggggtaa
     4861 aatattatta agtacatatg caaaagaaaa aggcatttct ccaatgaaaa taagatgtga
     4921 atatgatgag tatagtgaaa ataatttctt gaatcaagtt cttaaaaaag catgtatatc
     4981 tattttatgt agaataaatg acaattcaat tcaaggcaag attaaaaaaa ttttaagcta
     5041 ttttcaaaat gtagatttaa tttatataga tagaaaaaaa ctgttggatt ataaatttta
     5101 taaaaacaat gatagattta aagattgtta tttactagct agacttctac ttttaaattt
     5161 gtctatggat aatagtcaaa ataatcaaga ggcattttct atattatttg aaataaatac
     5221 tttgtatgaa gaatatatag gaattttaat caaaagtata tgggacaatt catttagaga
     5281 aacatatata caagataaga gtaaatttct tttgaaaaat gaacaaactg gtaagaaaaa
     5341 ctttaattta agaccggata tagttttata tgacttaaaa aatgaatatg aaattataat
     5401 tgacactaaa tggaaagcta ttgaagtaga ttcaaatgtt ttttataggt caagtgatat
     5461 ataccagatg tatgcatata taacagcata tgaaaatgca aaaagatgta tattattata
     5521 tccttgtatt caaaaagata agaattatag ttcctggaaa ttatcagaat catttaaggg
     5581 taagtttata gaagcaaaaa ctg
//
LOCUS       DAMJYR010000202         5551 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.207, whole genome shotgun sequence.
ACCESSION   DAMJYR010000202 DAMJYR010000000
VERSION     DAMJYR010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5551)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5551)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5551
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.207"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            50..274
                     /locus_tag="PC471_003621"
     CDS             50..274
                     /locus_tag="PC471_003621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2790704.1"
                     /translation="MIIKLTKDNFNFRFKQYEGMFFHAEVVEGMESIRYKITKDKEGT
                     ELKEFGSTHIKDNMIYVPQVEAYIDLNSIK"
     gene            327..851
                     /locus_tag="PC471_003622"
     CDS             327..851
                     /locus_tag="PC471_003622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDF2790705.1"
                     /translation="MSKPIDITIRPIRREDAQAFNEIRRTYSVMRNTLALMSDRVDKT
                     EAMLASLGENDYMFVAETNNNGEKDVIGFAGLHVNSSPRLRHSAEIGISVAENYQGRG
                     VGKKLMEQLLDIADNWIMLTRVGLEVIVDNEKGLNLYKKLGFEIEGTKKYAVIRDGKF
                     EDVYIMGRYNKNLI"
     gene            complement(956..1102)
                     /locus_tag="PC471_003623"
     CDS             complement(956..1102)
                     /locus_tag="PC471_003623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2790706.1"
                     /translation="MDMDLLFKIVFAIVAIGVSVKIIKTVASLAFKIALILLIILFVY
                     RMFL"
     gene            complement(1241..2167)
                     /locus_tag="PC471_003624"
     CDS             complement(1241..2167)
                     /locus_tag="PC471_003624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HDF2790707.1"
                     /translation="MNFSNIFIQVAVLFIIILVGYFVRKFNLLDDHCTSKLSTLTMTV
                     FLPSMIISSMQINFDSKMIQKILLLLFTSLIMYIVSIIIAFLLKYILKCNDKKDLGIY
                     QYIVVFSNVAFMGYPVIEAVLGHEAIFYTAIFNLPFNLFSFTLGIYLLSKGSTSRGFS
                     IKSLISPATIAIVIGLFLFVTGLRLPQFINEPLEMLGSITTPISMIIIGSLLANSSAL
                     DCFVNKKLYIVTFIRLLVLPVIVYFILKGWINDKMILAIPVVISSMPAAANTAIMANQ
                     YDSNITLASQCVFFTTLFSVISIPFISILLLS"
     gene            2357..4228
                     /locus_tag="PC471_003625"
     CDS             2357..4228
                     /locus_tag="PC471_003625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HDF2790708.1"
                     /translation="MKNTKFSIKKIKNISNDPKVVHTKGALLEKMGKMESAIELYKSA
                     ALDNYVKSQYTLGNIYESKKQFKEAEKWYSMAYKSGSEDAAFDLGNMYYKLDGYEYAI
                     YWYEKIANLGYLQAQNNLGVCHFKMKDFVRCEKWLKTAADKNLGKACFNLGVLYTFLE
                     KDDEAYEYYKKGSVLLDDDAKYNLAILNGKKKDNKSTVSLYKQLYKSGHMKGCFNLGM
                     IMEINDNLEDAEKYYKKSADRDDVKSEYRLAYVYDRKEELGDAIYYYEKAIEKNHTLS
                     KYRLANLYNRENEIEKAKTYYKMAAEDDITEAKNNLAGIYFEEKDYENAIKYYEDAIA
                     VGCKSSLENLGDLYYQNQDIEKAISYYSRIPNNVSCQIKLGNIYEDLNNIEEAISWYK
                     KASENGDTRSSYRLGCIYESLGNTKNARKYFEMASSKNHMNARIHLGRIYFREGKLEE
                     SKMMFDTPANENNVYAQHMVGLIYDMFYKDYVNSKFWYEKARAQGCVESIYNLGQIYL
                     KLNDDAEAEKYYKEGIKYGSKKCEYMLAGLYYKKSLDMYISLANEEYDNCEEIVEGFP
                     NLNIDFDEVLIPPFKLQEEVIDEEEYVPIYILNIKESLDSMLEGLETEMIIDEEHDS"
     gene            complement(4320..5255)
                     /locus_tag="PC471_003626"
     CDS             complement(4320..5255)
                     /locus_tag="PC471_003626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HDF2790709.1"
                     /translation="MIRYYKTIDSKLEKLSFFEDGCWINLVEPNHSEINEISNLLNID
                     VESIESALDEEERSRIDVEDNHTLILIDIPVDESDSNSSHYTTIPLGIILTEEAIVTV
                     CDAQTKILNDFIVGHIKDFFTFKKTRFLLQILHKNAAYYLHYLRKINKMTIIIEREIY
                     KSMKNKELVQLLELEKSLVYFSTSLKSNELVLNKMVRTAGIKKYPDDEDLLEDVIVEN
                     RQALDMAKIYGDILSRIMDAFSAIISNNQNNVMQILTVVTLIFSIPTIISGFFGMNVI
                     NMPFSNNPNGFWIILLITSIICIVITFFMSRNKLL"
BASE COUNT         2228 a          593 c          890 g         1840 t
ORIGIN      
        1 acaactaata ataagaaaaa tatcaattat ataaggagta gataaaaata tgataataaa
       61 acttactaaa gacaatttta attttagatt taaacaatat gaaggtatgt tttttcatgc
      121 tgaagtagtt gaaggtatgg aatccattag atataaaata actaaggata aagaaggtac
      181 agaattaaaa gaatttggaa gtacacatat aaaagataat atgatatatg tacctcaggt
      241 agaagcatac atagatttga acagtataaa ataaagatgt aaaattttaa ttaaaaataa
      301 agattatgtt tgagggggaa tccaatatga gtaagcctat agatataaca ataagaccta
      361 taagaagaga agatgcacaa gcttttaatg aaattagaag aacatacagt gttatgagaa
      421 atactttggc attaatgagt gacagagtcg ataaaactga agctatgtta gcttctttgg
      481 gagaaaatga ttatatgttt gtagctgaaa ctaataataa tggagaaaaa gatgtaatag
      541 gttttgctgg gttacatgta aattcttctc cacgattgag acatagcgca gaaataggaa
      601 taagtgttgc agagaattat cagggaagag gagtaggtaa gaagcttatg gaacaacttt
      661 tagatatagc agataattgg attatgttaa ctagagtagg attagaagta attgtggata
      721 atgaaaaggg attaaattta tataaaaagc taggttttga aatagaggga actaaaaaat
      781 atgctgtaat cagagatgga aaatttgaag atgtttatat tatgggaaga tataataaaa
      841 atttaattta aagaagctaa tagagaagta aaatagagtg tctttaataa aaaatttaca
      901 ttaaagataa tctattttac ttctctattg tgttaaaaaa cttattttta aatgtttaca
      961 aaaacattct atatacaaat aaaattatta gcaatatcaa cgctatttta aaagctaaac
     1021 tagcaacagt tttaatgatt tttacagaaa cacctatagc aactatagca aatactattt
     1081 taaagagtaa atccatatcc atatttaagc ctcctattct ataatacata tagatattat
     1141 tgacaaatat ctaggaaaaa attccatcaa tatactacaa taaaatgttt atatttgtaa
     1201 tttattgaat atgttattat ataatacata aaaaaataaa tcaagataag agtaaaatac
     1261 ttataaaagg tatagatata actgaaaata gtgttgtaaa aaacacacat tgtgaagcta
     1321 gtgttatatt ggagtcatat tgattagcca ttatagcagt atttgcagca gcaggcattg
     1381 aagaaattac tactggtata gctaaaatca tcttatcatt tatccatcct tttagaataa
     1441 aatacacaat cacaggaagt accaataatc ttataaaagt gactatatat aattttttgt
     1501 tgacaaaaca atcgagagct gaagaattag caagtaagct tccaatgata atcatggata
     1561 ttggtgtagt tatacttccc aacatctcaa gtggctcgtt tataaattgg ggtaacctaa
     1621 gtcctgttac aaataaaaaa agtccaataa caatagctat tgtagcaggg cttataagtg
     1681 attttattga aaaacctcta cttgtactgc ctttagataa aagataaatt cctaaagtga
     1741 aagaaaataa gttaaaaggc aaattgaata ttgcagtata gaatattgct tcatgaccta
     1801 aaactgcttc aattacagga taacccataa aagcaacatt tgaaaaaaca actatatact
     1861 ggtatattcc caaatctttt ttatcattac attttaaaat gtatttcaat aaaaatgcaa
     1921 ttataattga aactatatac ataatcaaag atgtaaataa taacaatagt attttttgta
     1981 tcattttact atcaaagtta atttgcatgg agctaattat cattgatggt aaaaaaactg
     2041 tcattgtcaa agttgaaagc ttagatgtgc agtggtcatc taataagttg aattttctga
     2101 caaaatatcc aactaaaata attataaata aaactgcaac ttggataaaa atattgctga
     2161 aattcataaa atgtcccccc tatatataca ttatatacca attacataat aaaatctaat
     2221 gattataaaa tttaaaattt aatgttacaa tatagtgaca aaaggtaaaa attcaatatt
     2281 atagcattaa ttgtagatta taatgtataa taatgtttga gagaatatat tttataaaat
     2341 gggtaggtag cgaataatga aaaatacaaa attttctata aaaaaaataa aaaatatatc
     2401 taatgacccg aaagtcgtac atactaaggg tgccctcctt gaaaagatgg gtaagatgga
     2461 aagcgcaata gagttgtata agagtgctgc attggataat tacgtaaaat ctcaatatac
     2521 tttaggaaat atatatgaaa gtaaaaaaca atttaaagaa gcagaaaagt ggtattcgat
     2581 ggcatataaa agtggaagtg aagatgctgc ttttgattta ggtaatatgt actataagtt
     2641 agatggctat gaatatgcta tatattggta tgaaaagata gcaaatttag gttatttaca
     2701 agcacaaaat aatttaggtg tatgccattt taaaatgaaa gattttgtaa ggtgtgaaaa
     2761 atggttaaaa acagcagcag acaagaattt aggtaaagct tgttttaatt taggggtatt
     2821 atatactttt ttggagaaag atgatgaagc atatgaatac tataaaaaag gttctgtgct
     2881 tttagatgat gatgcaaaat ataatttagc aatattaaat ggaaaaaaga aagataataa
     2941 atcaacagta agtttatata aacagttgta caagtcaggt catatgaaag gttgctttaa
     3001 cttgggtatg attatggaaa taaatgataa tttagaagat gcagagaagt attataaaaa
     3061 gagtgctgat agagatgatg tgaagtctga gtataggctt gcatatgtat atgatagaaa
     3121 agaagagctg ggagatgcaa tttattatta tgagaaagca atagaaaaaa atcatacact
     3181 ttctaagtat agacttgcta atttatataa tagagaaaat gagatagaga aggcaaaaac
     3241 ttattataaa atggctgctg aagatgatat aacagaagca aaaaataatc tggcaggtat
     3301 atattttgaa gaaaaagatt atgaaaatgc tattaaatat tatgaggatg ctatagctgt
     3361 tggatgcaaa agttcccttg aaaatctagg ggatttatat tatcagaatc aagatataga
     3421 aaaagcgatt tcgtattatt cgaggattcc aaataatgta tcttgtcaaa tcaaattagg
     3481 aaatatctat gaagatttaa ataatattga agaagcaata agttggtata agaaagcatc
     3541 tgaaaatgga gatacgaggt ctagttatag attggggtgt atatatgaga gcttaggaaa
     3601 tacaaaaaat gctagaaagt attttgaaat ggcaagtagc aaaaaccata tgaatgctcg
     3661 tatacatcta ggtagaattt attttagaga aggtaaattg gaagagtcta aaatgatgtt
     3721 tgatacacct gccaatgaaa ataatgtgta tgctcaacat atggtaggat taatttatga
     3781 tatgttctat aaagattatg taaattctaa attttggtat gaaaaggcta gagcacaagg
     3841 ttgtgttgaa tcaatatata atttaggtca aatttattta aaattaaatg atgacgcaga
     3901 agctgagaaa tattataaag aaggtattaa atatggaagt aagaagtgtg agtatatgct
     3961 tgcaggtctt tattataaaa agagcctaga tatgtacatt tctttagcaa atgaagagta
     4021 cgataattgt gaagagatag tagaaggctt tccaaattta aatatagact ttgatgaagt
     4081 attaattcct ccatttaagt tgcaagaaga ggtaatagat gaagaagagt atgtaccaat
     4141 ttatatacta aatataaaag aaagtttaga ttcaatgcta gaagggcttg aaactgaaat
     4201 gattatagat gaagaacatg atagttaaaa agtattataa gattatatag atttaaatat
     4261 ataaaagcca ttaggataaa tgtaaatcaa ttaattctaa tggccttttt attaatattt
     4321 tataatagct tatttcttga catgaaaaat gtaatcacta tacatattat agaggtaata
     4381 agaagtatta tccaaaatcc gtttgggtta ttagaaaatg gcatgttgat gacattcata
     4441 ccaaagaatc ctgatattat tgtaggtatt gagaaaatta gtgtaactac tgttaaaatt
     4501 tgcataacat tattctgatt attacttata attgcactga aagcatccat aattctactc
     4561 aatatatctc catatatttt agccatatca agagcctgtc tattttcaac tataacatct
     4621 tctaataggt cttcatcatc tggatacttc tttataccag cagttctaac cattttgttt
     4681 aaaacaagtt cattagattt tagtgaggta gagaagtata ccaaagattt ttctaattcc
     4741 aataattgaa ccaattcttt gtttttcatt gatttgtaaa tttctcgctc tattatgatt
     4801 gtcattttat ttatttttct taagtaatgc aagtaataag ctgcattttt gtgaagaatt
     4861 tgaagcaaaa agcgagtctt tttaaacgta aaaaaatctt taatatgacc aactataaag
     4921 tcatttaaaa tcttagtttg tgcatcacaa actgttacaa tagcttcttc agttagtata
     4981 atacctaagg gtattgtagt ataatgagaa gaattagagt cactttcgtc tactggtata
     5041 tctataagta taagagtatg attgtcttca acatctattc ttgagcgttc ttcttcatct
     5101 agtgcagatt ctatgctttc tacatctata tttaataaat tagaaatttc gttgatttca
     5161 ctatgattag gttcaacaag atttatccaa cagccatctt caaaaaaact aagtttctct
     5221 aattttgaat ctatagtttt gtaatatctt atcatggcaa cacatccttt attcagtttt
     5281 atataaatct catcatttaa tagtatctat taattaacta atttatataa ctgaatatat
     5341 gtattagttt tgattttaga tattattaag tgatgaaaat gtaaaaaatt ctctccgcaa
     5401 cggactagta tccattgccc atcactctcc ttatttatta ttttatatat agctatttat
     5461 gaaatgcgta ttttaagcac tctttaatat aatacctttt ccaggtatat aaatcaagtt
     5521 ttgatttgat taaataatat ttttaccata a
//
LOCUS       DAMJYR010000203         5533 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.208, whole genome shotgun sequence.
ACCESSION   DAMJYR010000203 DAMJYR010000000
VERSION     DAMJYR010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5533)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5533)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5533
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.208"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            55..171
                     /gene="rrf"
                     /locus_tag="PC471_003627"
     rRNA            55..171
                     /gene="rrf"
                     /locus_tag="PC471_003627"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(385..1569)
                     /locus_tag="PC471_003628"
     CDS             complement(385..1569)
                     /locus_tag="PC471_003628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HDF2790710.1"
                     /translation="MLSKRLNFITPSYTIGISSKVKEMESNGVKVINLSIGEPDFNVP
                     NNAKSYGIDSLNKDYTKYDLVPGLKILREEICKKLIEENNCNYSIDEIVVSSGAKNSI
                     TNTLLALTDEGDEVLLPKPYWVSYPEMVKLVNAVPVFIDTKKENGFKLTKDELEKSIT
                     DKTKILVINNPSNPTGSVYTKDELIEIVDVCIQNKIYILADEIYEKICYTGEFTSIAS
                     LSEEAKDITITINGFSKSAAMTGLRLGYTASNKTIAKAMSSIQGHLISHPSLTAQYIA
                     YGALKDCSIDIDNMVKTYKSRRDLIKSKLDSIDNVGYVNPNGAFYIFIDLSKVSEKFE
                     YKDSFSIEFCNQFLEEYNVAVVPGIAFGMDKYIRISYACSENTFLSGLDKLKEFVYKI
                     MA"
     gene            1942..4293
                     /locus_tag="PC471_003629"
     CDS             1942..4293
                     /locus_tag="PC471_003629"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside triphosphate reductase"
                     /protein_id="HDF2790711.1"
                     /translation="MVEFVKKRDGRVIPFNEDRITRAIFLAATNVAEREGIVPDYKLS
                     EQLTQEVIKFLNHKYSESVPSVEDIQDSVVKVLIETGHAKTSEEYIIYRTERSRIRNS
                     KTRLMKAIEEITFEDAEDADIKRENANINGNTAMGTMLQYGSTVSKEFCKTHILKPEH
                     SFAHDNGDIHIHDMDFLNMGTLTCCQIDVKKLFNGGFSTGHGFLREPQDIISYGALAA
                     IAIQSNQNDQHGGQSIPFFDYGLAEGVYKTFKKFYIGNLAKALKLFKGIENNDVIKNI
                     VYNTEKETNQKVGLKRDELYLNLEKEKLIQTFDIDDELVNKMQNFAFEESYRETDKKT
                     YQSMEAFIHNLNTMHSRAGAQVPFSSVNFGTDTSEEGRMVTKNLLLSQERGLGNGETP
                     IFPILIFKVKEGINLNPEDPNYDLFKLSCRVSAKRLFPNFSFLDAPFNAKYYKKGEPD
                     TEATYMGCRTRVLSNVCGSETVSGRGNISFTTVNLPRLGIKHGIINNEKANLDGFFEE
                     LDEKINLIIEQLLERLEVQGNKKMKNFPFLMGQGVWKGSDDLGPEDTLKEVIKQGTLT
                     IGFIGLAECLIALIGKHHGESKEAQELGLKIVSHMRHKMDEATDKYKLNFSLMGTPAE
                     GLSGRFTKIDKKVYGEIKGITDKEYYTNSFHVPVYYNISAYDKIEIEAPYHELTNAGH
                     ITYVELDGDPSDNLEAFETVIKAMKDLGIGYGSINHPVDRDPICGFSGVITSNICPVC
                     GRNEDESDIKFERIRRITGYLVGTVDRFNNAKKAEVRDRVKHR"
     gene            4315..4854
                     /gene="nrdG"
                     /locus_tag="PC471_003630"
     CDS             4315..4854
                     /gene="nrdG"
                     /locus_tag="PC471_003630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HDF2790712.1"
                     /translation="MKIRMSSTISYDSIVDGPGLRMVIWTQGCIHNCKECHNPQTHDL
                     CGGFYMDTEEIINKVKSLKLQKGITLSGGEPFLQPEPLEEIAREAKINGLDVWSYTGF
                     TFEQLLDKKNRAYFKNLNLLKQIDILVDGKFIAEKKDISLKFRGSSNQRIIDVQKSLK
                     YKKVFLVEQYMKDDLSIAE"
BASE COUNT         2156 a          628 c          891 g         1858 t
ORIGIN      
        1 ctaggttcta taaacttatt aaaaacttaa tagaaatatt aagcataaag attatgtggt
       61 tacaatagca gagaggatac acctgttccc attccgaaca cagaagttaa gctctctagc
      121 gctgatggta cttggtggga aactgcctgg gagagtagga cgtagccacg taattttttt
      181 gttataataa atttagaatt taacttatat agtttaattt gataatttga tgaaaaaaat
      241 aaaagactta ctgattttta aatctatagg tcttttattt tttatctaac atttaaatta
      301 agttgttgaa tatatcttat attacttacc ttatatatta attaccatta atacattata
      361 tctaattact attcatctaa attattatgc cattatttta tatacaaatt cttttaattt
      421 atctaatcca gataagaaag tattttcact gcaagcatag gatatacgaa tatatttatc
      481 cataccaaat gctatgccag gaacaactgc aacattatat tcttctaaaa attgattaca
      541 aaattctata gaaaaactat ctttatattc aaatttctca gatactttag aaagatctat
      601 aaatatatag aatgcgccat ttggattcac atatcctaca ttatcaatag agtctagttt
      661 tgatttaatt aaatctcttc ttgatttata tgttttaacc atattgtcta tgtcaattga
      721 gcaatctttc aaggctccat aggctatgta ttgtgctgtt agacttgggt gagatataag
      781 atgtccttgt atagaactca ttgctttagc aatcgtttta ttagatgcag tatatcctaa
      841 tcttaatcca gtcattgcag ctgacttaga aaaaccattt atagttattg ttatatcttt
      901 agcttcttcg cttaatgatg caattgatgt aaattcacca gtgtagcata ttttttcata
      961 tatttcatct gctaaaatat atattttatt ttgtatgcat acatctacta tttctattaa
     1021 ttcatctttt gtataaacac taccagtagg atttgaagga ttgtttatta caagaatttt
     1081 tgttttatct gttatagatt tttcaagttc atcttttgtt aacttaaatc cattttcttt
     1141 tttagtatca ataaaaactg gaacagcatt aactaactta accatttcag gataactaac
     1201 ccaataaggc tttggtaata agacttcatc tccttcgtct gttaaagcta gtaaagtatt
     1261 tgttattgag ttttttgctc cacttgatac aactatttca tctattgaat agttacagtt
     1321 attttcttca ataagttttt tacagatttc ctctctaagt atttttaatc ctggaactaa
     1381 atcatattta gtgtaatctt tatttaaaga atcaatacca taagattttg cgttattagg
     1441 tacattaaaa tcgggttctc ctatactaag atttattact ttaactccat tactttccat
     1501 ttctttaact ttagaactaa tacctattgt gtaggatggt gttataaagt ttagtctttt
     1561 tgataacata aaatgcctcc ttttaaatta ttatttatat tatacgacaa atttaaagaa
     1621 tttacataag gaaatttgta gatatataca ttaatatata taatagccta cactttttgc
     1681 ctaaaattag tgtaaaaaaa agagaaaaaa attttaactt tcaaagttgt tagaaacgct
     1741 actgttcaaa atatccacat agtatttgca atgatttgcg caagtttgac tattatgtac
     1801 aaatttggta atcttaatat agcaattatt aagataatcc ctttaactta gttgaagggg
     1861 atttttttag acttaacata ctacatatag tggtatgggg attaataaaa tacaatatat
     1921 agtaacagga ggttttttta attggttgaa ttcgtaaaaa aaagagatgg aagagtaatt
     1981 ccatttaatg aagatagaat aacaagagct atatttttag cagcaacgaa tgttgcagaa
     2041 agagaaggaa tagtacctga ttataaatta tctgagcaat taactcaaga agtaataaag
     2101 tttttaaatc ataaatactc agaatcagtt ccaagtgttg aagatataca agattctgta
     2161 gttaaggttc ttatagaaac tggccatgca aaaactagtg aagaatatat aatatacaga
     2221 actgaaagaa gtagaataag aaattcaaaa acaagattga tgaaggcaat agaggaaatt
     2281 acctttgaag atgcagaaga tgctgatata aaaagagaaa atgcaaatat taatggtaat
     2341 acagcaatgg gaactatgtt acaatatgga agcactgtat ctaaagaatt ttgtaaaaca
     2401 catatattaa aaccagagca ttcttttgct catgataatg gagatataca catacatgat
     2461 atggattttt taaatatggg aacattaact tgttgtcaaa tagatgttaa aaaactattc
     2521 aatggtggat tttcaacagg acatggcttt ttaagagagc cacaagatat aataagttat
     2581 ggtgcattag ctgcaattgc tatacaaagc aatcaaaacg atcaacatgg aggtcaaagt
     2641 ataccatttt tcgattatgg attggctgaa ggtgtatata agacatttaa gaaattctac
     2701 ataggaaatt tagctaaagc attgaagtta tttaaaggaa ttgaaaataa tgatgtgata
     2761 aagaacatag tatataatac agagaaagaa acaaatcaaa aagttggtct taagagagat
     2821 gaattgtatc taaacttaga aaaagagaaa ttaatacaga cttttgatat agatgatgaa
     2881 cttgtaaata agatgcaaaa ctttgcattt gaagaatcgt atagagaaac tgataaaaaa
     2941 acatatcaat ctatggaagc atttatacac aatctaaata caatgcactc tagagcaggt
     3001 gcacaggtac cattttcaag tgtaaacttc ggaacagata cttctgaaga aggaagaatg
     3061 gtaactaaga atcttctatt atctcaagaa agaggtttag gtaatggaga gacaccaatt
     3121 tttccaattt taatatttaa ggtcaaagaa ggcataaatt taaatcctga agaccctaat
     3181 tatgatttat ttaaattatc atgcagagta tcagctaaaa gactattccc taattttagc
     3241 tttttagatg cgccttttaa tgcaaaatat tataaaaaag gtgaaccaga tacagaggct
     3301 acatatatgg gttgtagaac aagagtactt agtaatgtgt gtggttcaga aacagttagt
     3361 ggtagaggaa acatatcttt tactacagta aatttaccaa gattgggtat aaaacatggt
     3421 attataaata atgaaaaggc taatttagat ggtttctttg aagaattaga tgagaagata
     3481 aacttaatta tagaacaatt attggagcgc cttgaggtac aaggaaataa aaagatgaag
     3541 aattttccat tcttaatggg gcaaggtgta tggaaaggtt ctgatgattt aggaccagaa
     3601 gatacactaa aagaagtaat aaaacaagga acattgacaa ttggatttat aggtttagca
     3661 gagtgcctta ttgctcttat aggtaaacat catggagaaa gtaaagaagc tcaagaatta
     3721 ggattaaaaa tagtgtcaca tatgagacat aaaatggatg aagcaacgga caaatataaa
     3781 ttaaatttct ccttaatggg tactccagca gaaggtcttt caggtaggtt tactaaaata
     3841 gataaaaaag tttatggaga aattaaaggt atcacagata aagaatatta tactaattca
     3901 ttccatgtac cagtttatta caatataagt gcatatgata aaattgagat agaagctcca
     3961 taccatgaat tgactaatgc aggtcatata acatatgtag agttagatgg agatccatct
     4021 gataatttag aagcttttga aactgttata aaagctatga aagatttggg tataggatat
     4081 ggaagtataa atcatcctgt agatagagac ccaatatgcg gtttttcagg tgtaataaca
     4141 agtaatatat gccctgtatg tggaagaaat gaagatgaaa gtgacatcaa attcgaaaga
     4201 ataagaagaa taacaggata cttagttggt acagttgata gatttaataa tgctaaaaaa
     4261 gcagaagtaa gagatagagt aaaacataga tagttttatt tgggggaata agttatgaaa
     4321 ataagaatgt catctactat aagttatgac agcatagttg atggaccagg attaagaatg
     4381 gtaatatgga ctcaaggatg tattcataat tgtaaagagt gccataatcc tcagacacat
     4441 gatttatgtg gaggatttta tatggataca gaagaaatta taaataaagt caaatcattg
     4501 aaattacaaa aaggcattac gctatcagga ggagaaccat ttttacaacc agaaccatta
     4561 gaagaaatag caagagaagc taaaataaat gggttagatg tatggtcata tactggattt
     4621 acgtttgaac aactgttaga taaaaaaaat agagcgtatt ttaaaaattt aaatttacta
     4681 aagcaaattg atattttagt ggatggaaaa tttatagctg aaaaaaaaga tataagctta
     4741 aagtttagag ggtcatcaaa tcaaaggata attgatgttc aaaaaagttt gaaatataaa
     4801 aaagtgtttt tggttgagca atatatgaag gatgatttat ctatagcaga ataaaatata
     4861 aataaacagg attaaatata tgcatatatt taatcctgtt tattttcata taatatttca
     4921 agcttgtata atgtaacaat taaaccaaaa gactcattgt atatttgaaa ttaagttttt
     4981 tatagtttat aggtgtttta tgtggattac gaatgatata gtattgatac attatttaat
     5041 agattcttat gccaagaaac aattaattta tagaaattct taattgttac aatataaata
     5101 tctagataag ttataagata ctgataaata atttataaag tttattaatc taatattgtg
     5161 tgtgaatatt aagtggtcaa gtagtctaaa aaatgaaaaa gatttaaaaa agtgttttaa
     5221 ctaaacacat aagtgttaaa ttcatgtata agtataggtt agatacataa gaaataatta
     5281 tgtaatatct atctttgtaa aaaaatatta aattttgtaa tttgtggata aatatgttta
     5341 taatgtggat aatatttaat gtaataagtt aaagcaatat gaatcaactg tttataatgt
     5401 ggataatatt ataaatgtat gaataattgc agtatttaaa aacaaaaaaa gttaaaaaag
     5461 ttgttgactc taaaagaaaa agttggtata gtaatacttg tcctcaagaa aagggcaaac
     5521 aaataaagca taa
//
LOCUS       DAMJYR010000204         5426 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.209, whole genome shotgun sequence.
ACCESSION   DAMJYR010000204 DAMJYR010000000
VERSION     DAMJYR010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5426)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5426)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5426
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.209"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            180..1670
                     /locus_tag="PC471_003631"
     CDS             180..1670
                     /locus_tag="PC471_003631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HDF2790713.1"
                     /translation="MFQFETQIHKLRHDVLTAIARLGKEDNLTREAMDNIQYEIIKGE
                     KPTYRCCVYKERAIISERVNVSMGLQPGQDKIDLDTMDIEEDEQIIYILEAACDSCPI
                     NEFTVTDICRGCLAHRCKEACKFGAITHVGGMAYINHELCKACGMCKKACQYDAISEV
                     VRPCKSVCPTNALGFDRENMKAMIHEDKCLNCGACMSACPFGAISDKSLIAPVARKLV
                     QKEKMYAVVAPAITGQVEANVTYGQIKNAIKSLGFVDVLEAACGADAVTVHESLEFAE
                     RMEKGEAFMTNSCCPGFVGYIEKTYPNHVGKISSTVSPMIASGRFIKSHEPDAKVVFI
                     GPCTAKKSEAAKKELKGAVDYVLTFEEIMALFEAFDIDLAKCSNEDIDDASIFGRGFG
                     AAGGLTKAIENYLAEKGIGVNFEPVKISGSREIKKTMTMANLGKLEGNFIEGMMCEGG
                     CINGSGKFLSGAKVKATFDRKNSQSTKKSVLANTKIKDFSNINLEK"
     gene            1950..3446
                     /locus_tag="PC471_003632"
     CDS             1950..3446
                     /locus_tag="PC471_003632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HDF2790714.1"
                     /translation="MFEFETQLKRLKHEVLTAVARLSIEENLTKENIEKIPYEIIKGE
                     KARYRCCVYKEREVVLERAQLALSLTPNSKYGDINPDSVSLDTDEQIIYIVEAACDRC
                     PINEFTVTELCRGCLAHRCKEACKFGAISYINGRAYINHDLCKACGMCKSSCQYDAIS
                     EVVRPCKSVCPTGALDFNRNTMKAMIHEDNCINCGACMSACPFGAISDKSLIAPVAKK
                     LAKKENMYAIVAPAITGQIDSNITYGQLKNAIKSLGFVDMIEASCGADAVTVHESNEL
                     IERIKSGDNYMTNSCCPGFFKYIEKMFPDEKDRISSTVSPMVATGRLVKKIDSDAKVV
                     FIGPCTAKKSEATEKSLKDSVDYVLTFEELMALFEAFNIDLSKCSNEDINDGSIFGRG
                     FGASGGLAKSVQNYIAEKGLSFDFEPIKVSGPRQIKKTMTMAKLGRLEGNFIEGMMCE
                     EGCINGAGKFLSGVKAKNTFDRKNGHSTKKSVLSNDKIKEFEDVNLER"
     gene            3580..4506
                     /locus_tag="PC471_003633"
     CDS             3580..4506
                     /locus_tag="PC471_003633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDF2790715.1"
                     /translation="MRKIVLKDILFLVTTLSIIVILLLVTNITTNSKIKSTSESYLKS
                     DKNSVRQNGIQRSTKSSTKNTAFKTTILNTGKSDCILVEIGNKVIMIDTGEDKNGKQI
                     VDRLKEKGINTLDYLILTHLDKDHIGGVDSVLSSVKVKNIIQANYKKDSKQYDEYIDS
                     LKKADIEPVLLKDNMNIVINGAEINIHPASKSKYESSNDYSIITNISYGAHKFLFAGD
                     AEEERLSEFINGNTLKYDFVKMPHHGRYDKLTETFLESISPQYAVITCSEKKEPEEGV
                     LKILERLNIKTFLTSNGEVVINSDGKTLSVNQ"
     gene            4624..4815
                     /locus_tag="PC471_003634"
     CDS             4624..4815
                     /locus_tag="PC471_003634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2790716.1"
                     /translation="MLDRFFGEGGFGGCAWWIIILFFLFLAFQDCWAEMDLCAWIPFL
                     ILLLILCECGDVFDGGCGC"
     gene            5038..5388
                     /locus_tag="PC471_003635"
     CDS             5038..5388
                     /locus_tag="PC471_003635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2790717.1"
                     /translation="MAIVDKIFNEIKLMVARLLLGKKYSEYEEKDLVYNTEEEVILIT
                     LKRLVFQGNVNEAEEILFDKAKSVNSENMQYIAIEFYTMLMEKTDEELEAMNFSKQEV
                     YQGIEDIRKVLNLE"
BASE COUNT         2162 a          542 c         1034 g         1688 t
ORIGIN      
        1 aaaaataaaa atagtgaaat ttcagaaata tcaataatat tatataataa aacaaaaaaa
       61 cactatatat attcgataaa ataaaattta aacatataat attttatgta tatattaata
      121 aaaggacata ttgtgttata atggtatcgt ataaaatcat ttacgggggt ggtattaaaa
      181 tgtttcaatt tgaaacacaa atacacaaat taagacatga tgttttaact gcaatagcta
      241 gacttggaaa agaagataac cttaccagag aagcaatgga taatattcag tatgaaataa
      301 taaaaggtga gaaacctaca tacagatgtt gtgtttataa agaaagagct ataatatctg
      361 agagagtaaa tgtaagtatg gggttacaac caggtcaaga taagatagat ttagatacaa
      421 tggatataga agaagatgaa caaattatat atattttgga agctgcatgt gacagttgtc
      481 caataaatga atttacagta acagatattt gtagaggatg tctagcacat agatgtaaag
      541 aagcttgtaa atttggagct ataacacatg tcggaggaat ggcctatata aatcatgaat
      601 tatgtaaggc ttgtggaatg tgtaaaaaag cttgtcaata tgatgccatc tcagaggttg
      661 ttagaccatg taagagtgtt tgtccaacta atgccttagg atttgataga gaaaatatga
      721 aagctatgat acatgaggat aaatgtttaa actgtggagc atgtatgtca gcatgtccat
      781 ttggagcaat atctgataag agtttgatag ctcctgtagc tagaaagtta gttcaaaaag
      841 agaaaatgta tgcggtggta gctccagcta taacaggtca agttgaagca aatgttacat
      901 atggtcaaat aaaaaatgct ataaaatcac taggatttgt tgatgtatta gaagcagctt
      961 gtggagcaga tgcagttaca gttcatgaaa gcttagaatt tgctgaaaga atggaaaagg
     1021 gagaagcttt tatgacaaat tcttgttgtc caggatttgt tggttatata gaaaaaacat
     1081 acccaaatca tgtagggaaa atatcttcta cagtatctcc tatgatagct agtggtagat
     1141 ttataaaaag tcatgaacca gatgctaaag ttgtattcat aggaccatgt acagccaaaa
     1201 agagtgaggc agctaagaaa gaattaaaag gagctgttga ttatgtactt acttttgaag
     1261 aaataatggc attatttgag gcatttgata tagacttggc taagtgttca aatgaagata
     1321 tagatgatgc atcaattttt ggaagaggtt ttggagctgc tggtggtctt acaaaagcta
     1381 tagaaaatta tcttgctgaa aaaggtattg gtgttaattt tgaacctgta aagatttctg
     1441 gttcaagaga aatcaagaaa actatgacta tggctaattt aggaaagcta gaaggtaatt
     1501 ttatagaagg aatgatgtgt gaaggtggtt gtataaatgg ttctggtaaa ttcttatcag
     1561 gtgcaaaggt taaagccact tttgatagaa aaaattctca atcaactaag aaaagtgtct
     1621 tagcaaatac aaagataaaa gattttagta atataaattt agaaaaataa agataataca
     1681 aagcaacaaa aatacatatt gttaatagag ttgttctaag ttgaggttta acttttggac
     1741 aactctattt ttatttatat atttaattat tgaacaacta aaataaaaat tatatacact
     1801 taaaataaaa attacatata taatattttg tatataactt aacaaagtat atgcctatgt
     1861 tataataatt tatagaaata aataaaaaat acaatataca taatagtaga aatatagcaa
     1921 aaaggtgtga tttataggga ggattactta tgtttgaatt tgaaacgcaa ctaaaaaggt
     1981 taaaacatga agttttaaca gcagtagcaa gactatctat tgaagaaaat ttaacaaaag
     2041 aaaatataga aaaaattcca tatgaaataa ttaaaggaga aaaggcgaga tatagatgtt
     2101 gtgtttataa agagagagaa gtagttcttg aaagggcgca acttgcatta agtttaactc
     2161 caaatagtaa atatggagat ataaatccag attcagttag tttggacaca gatgagcaaa
     2221 ttatatatat agttgaagca gcatgtgata gatgtccaat aaatgaattt acagttacag
     2281 agctttgtag agggtgtttg gctcatagat gtaaggaagc atgtaaattt ggggcaattt
     2341 cttatataaa tgggagagca tatataaatc atgatttatg taaggcttgt ggaatgtgca
     2401 aaagttcatg tcaatatgat gctatatcag aagttgtaag accttgtaaa agcgtttgtc
     2461 caacaggagc tttggatttt aatagaaaca ctatgaaagc aatgatacat gaagataatt
     2521 gtataaattg tggagcatgt atgtcagcat gtccttttgg agcaatatct gataagagtt
     2581 tgatagcacc tgttgcaaaa aaacttgcaa agaaagaaaa tatgtatgca atagtagctc
     2641 cagctataac aggtcaaata gatagtaata ttacatatgg tcagttaaaa aatgctataa
     2701 aatcactggg atttgtagat atgatagaag cttcttgtgg agcagatgca gttacagttc
     2761 atgaaagcaa tgaacttatt gaaagaataa aatcaggaga taattacatg acaaattcat
     2821 gttgtccggg attttttaag tatatagaga agatgttccc agatgaaaaa gatagaatat
     2881 catctacagt gtctcctatg gtagcaacag gaagattagt aaaaaaaata gattctgatg
     2941 ctaaagttgt atttataggt ccatgtacag ctaaaaagag tgaggcaaca gagaaaagtt
     3001 taaaagattc agtagattat gtacttactt ttgaagaact aatggcatta tttgaagcat
     3061 ttaatataga tttatcaaaa tgttcaaatg aagatataaa tgatggttcg atttttggaa
     3121 ggggatttgg agcatctggt ggacttgcca aatcagtaca gaactatatt gctgaaaagg
     3181 ggcttagttt tgattttgaa cctataaaag tatctggtcc aagacaaatt aaaaagacaa
     3241 tgacaatggc taaattggga agattagaag gtaattttat agaaggaatg atgtgtgaag
     3301 aaggttgtat taatggagcc ggtaagtttt tatcaggtgt aaaagccaaa aatacttttg
     3361 atagaaaaaa tggacactct actaaaaaga gtgttttatc taatgataag attaaagaat
     3421 ttgaagatgt aaatttagaa agataattgt tttggtacag tcattttttt attatgccac
     3481 aaatatgcct aaaatttgcc ctttttatat gtgtatttta cagagttaag gttgctatac
     3541 tataaatatg taataaataa aatttatagg agcttttgga tgaggaaaat agtattaaaa
     3601 gatatcctgt ttttagttac tacattatct ataatagtaa tattattgct tgtaacaaat
     3661 ataactacta atagtaagat aaaatcaaca agtgaatctt atttaaaaag tgacaaaaat
     3721 agtgtgaggc aaaatggtat ccagaggagc acaaagagta gcacaaaaaa tactgcattt
     3781 aagactacta tactaaacac tggaaagtct gattgcatat tggttgagat aggtaacaaa
     3841 gtcataatga ttgatacagg agaagataaa aatggaaaac aaatagtgga taggttaaaa
     3901 gaaaaaggaa taaatacact tgactatttg attttaactc atttagataa ggaccatata
     3961 ggtggagtag atagtgtgtt atctagtgtt aaagtaaaga atattataca agcaaattat
     4021 aagaaagata gtaagcaata tgatgaatat attgactcac ttaaaaaagc tgatatagaa
     4081 ccagtgctac taaaagataa tatgaatatt gtaatcaatg gtgcagaaat aaacatacat
     4141 ccggcttcaa aaagcaagta cgagagttca aatgattatt caataatcac taatataagc
     4201 tatggagcac ataagttttt atttgcagga gatgcagagg aagaaagatt gtcagagttt
     4261 ataaatggaa atactttaaa atatgatttt gttaaaatgc ctcatcatgg aagatatgac
     4321 aaattaactg aaacattttt agagtctatt tcacctcaat atgctgttat tacatgttca
     4381 gagaaaaaag aaccagagga aggtgtttta aaaatattgg aaagattgaa tataaaaaca
     4441 tttttgactt ctaatggaga ggtagttatc aatagtgatg gtaagacatt gagtgtgaat
     4501 caataatttt tttaaaaata tctaaaaaaa tttaaaaaaa tcattagcaa atattacccc
     4561 ttaaacatag tatattttag gagctgaaac tccacataca aaatgactag gaggtaacat
     4621 attatgttgg atagattttt tggtgaaggc ggatttggtg gatgtgcttg gtggataata
     4681 atattattct tcttattctt agcattccaa gattgttggg ctgaaatgga cttatgtgca
     4741 tggatacctt tcttaatatt attattaata ctttgtgaat gtggtgacgt ttttgatgga
     4801 ggatgtggat gctaaaaaaa tcaatttaga tgatttaata aagcttatgg tgggtataaa
     4861 taaaatacca aaccaatcac aatctagtgt ttgaaggtag tcggaaatcc cccgctacct
     4921 tctttttttt attgttaaaa tttatttttg aattatttca tttacatata ataattttta
     4981 cgataaagtc tgatataatt aaaaagaaaa taaaatttat ttataggaga tgtaaaaatg
     5041 gctatagtgg ataagatttt caatgagata aaacttatgg ttgcaagact tttattggga
     5101 aaaaaataca gtgaatatga agaaaaagat ttagtgtata atacagaaga agaagttata
     5161 cttataactt taaaaagatt agtttttcag ggaaatgtaa acgaagcaga agaaatttta
     5221 tttgataaag ctaagagtgt aaatagtgaa aatatgcagt atatagcaat agaattttat
     5281 actatgctta tggaaaagac tgatgaagaa ttagaagcta tgaatttttc taaacaagaa
     5341 gtttatcaag gtatagaaga tattagaaaa gtattgaatt tagaataaaa gatatatgaa
     5401 aatattgaaa taaattaatg ctaagg
//
LOCUS       DAMJYR010000205         5338 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.210, whole genome shotgun sequence.
ACCESSION   DAMJYR010000205 DAMJYR010000000
VERSION     DAMJYR010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5338)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5338
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.210"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(15..917)
                     /locus_tag="PC471_003636"
     CDS             complement(15..917)
                     /locus_tag="PC471_003636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HDF2790718.1"
                     /translation="MYNKIKYIPINCEIACNKLKRSMPYKWDLNIYRGCEHGCKYCYA
                     IYSHKYINSNNYFEEIYVKTNIVEKLERQLKSSKWKKEVINIGGVTDSYQPIEADYKI
                     MPEILNLLIKYKTPAIISTKSDLILRDYDLIDKLSRITYINVASTITTVNEDTQRLIE
                     PNGVDSMRRFKMLKEFRKTNASVGLHIMPIIPYITDDFDNINSLFRHAKECNVHYVLP
                     GTLYLRGITRGVFFDFVKKEFPELFDKLSILYSEGSLNREYKNQLYKMVNELRYKYSL
                     SSSYSKIMKEKLKNSGDVQISFFD"
     gene            complement(967..1428)
                     /locus_tag="PC471_003637"
     CDS             complement(967..1428)
                     /locus_tag="PC471_003637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine dimer DNA glycosylase/endonuclease V"
                     /protein_id="HDF2790719.1"
                     /translation="MRLWHKDLIDVLPKNQLVSQWRELLAIKGSIDKKGTPNHLLVNK
                     VLNYSIDEFKFYTKIVHDEMLKRNYKPNELKYTSILKWKNRNFANDISNEHSLNLENL
                     YDDWHNKMYLKQCLYNLEEKATCGGIPINEWNILLCKYSKDYELWSGNIMF"
     gene            complement(1480..1935)
                     /gene="def"
                     /locus_tag="PC471_003638"
     CDS             complement(1480..1935)
                     /gene="def"
                     /locus_tag="PC471_003638"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HDF2790720.1"
                     /translation="MAIRKIRTFDDEILRKKSKYVENVDNKIREILNDMAETMYNTPN
                     GGGLAACQVGVLKRLVVIDLGEGLIKLVNPEIIKQEGEQIVVEGCLSFPEVWGKLKRP
                     KKVTVQALNEYGEKIEIKGSGFMAKCLCHEIDHLNGIVFTDKIIEHVKL"
     gene            complement(1907..2170)
                     /locus_tag="PC471_003639"
     CDS             complement(1907..2170)
                     /locus_tag="PC471_003639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2790721.1"
                     /translation="MKNKIKILREKLGLTQEQLGRLVGTSRQAINAIETGKNEPSIWL
                     AYDISRIFNEPIESVFLFEESERKSRAQISRGEYYGYKKDKNI"
     gene            complement(2267..2983)
                     /locus_tag="PC471_003640"
     CDS             complement(2267..2983)
                     /locus_tag="PC471_003640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2790722.1"
                     /translation="MIKIINAFKQEFEQIKRDAMLFIVCVSPILCGVFIKFGIPLIQN
                     ISLNKFYYQLNLEPYFLMFDLLLAFITPFMFFFASTMVILGEIDDSISRYLIVTPLGK
                     TGYLISRFGIPGILAFIITMILLIFFSLTKISFLLNLSISLLVLLQGIIISILVISLS
                     SNKLEGMVITKFSGVFMMGILAPFFILNKAQYILFFLPTFWISKAFKEDNYVYMFISI
                     VISLIWILLLFKKFSKKIVK"
     gene            complement(2980..3663)
                     /locus_tag="PC471_003641"
     CDS             complement(2980..3663)
                     /locus_tag="PC471_003641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2790723.1"
                     /translation="MRLKNLILGDIKFQFKYGFYFLYLFLSIIYICIINVFPTFMREK
                     IAIIMIYSDPAAMGLFFMGAIVLLEKSQRVLNSLAVSPVKVSEYILSKVISLGVISSI
                     VAMFIAITLNLDNIIISTIGTFFSSIIFSLLGLILASKASSLNQFIVLSIPIEIICFI
                     PPILNVLLDTKSYANLYPFNICISLISGDKNFIMINILILISIIIIIYFITYYFICSS
                     WKKVGGVKL"
     gene            complement(3660..4505)
                     /locus_tag="PC471_003642"
     CDS             complement(3660..4505)
                     /locus_tag="PC471_003642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2790724.1"
                     /translation="MIKVDDLSFSYTERDFLQNINFGVGKGEILGFLGPSGAGKSTLQ
                     KILIGMITNYGGSVIVNGVESKRHSNKFYENIGVDFEFPSLYEKLTAIENLKYFGSLY
                     SKKLLSIDELLKSVGLENEANKRVSEYSKGMKSRLNFIKALLHNPDILFLDEPTSGLD
                     PSNSKVMKDIILSEKSKGKTIILTTHNMLDATELCDRVAFIVNGKISALDTPHNLIMS
                     KGAIKVRYTYFDNGEKTSECFLNNTANDKNLNMLIEKNKLLSIHSSEPTLNDIFIEIT
                     GRNLQ"
     gene            complement(4619..5209)
                     /locus_tag="PC471_003643"
     CDS             complement(4619..5209)
                     /locus_tag="PC471_003643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDF2790725.1"
                     /translation="MPKSYSDKEREYIIKRLKEEARLCMEQYGIRKTTVDELVKRVKI
                     PKGTFYLFFQSKELLFFEVLRDIHDSIQKEILYEINRVDESITCEQLTDIFMKFYRMV
                     DSTSILNLMINGEFEILVRKLPDSIIEEHFRHDDFEIGEIISCIPNAKNKDIESFSGA
                     FRAVFLTMLYKREVGSNCFEDALRLMINGLVIQLME"
BASE COUNT         1875 a          855 c          512 g         2096 t
ORIGIN      
        1 ttttttatat aaaactaatc aaaaaaagat atctgtacat ctcctgagtt ttttaacttt
       61 tccttcatta ttttagagta actgcttgat agagaatatt tatatctaag ttcattaacc
      121 attttataca attgattttt atactcccta ttaagtgaac cttcactata tagtattgat
      181 aatttatcaa ataattcagg aaactctttt ttaacaaagt caaaaaaaac acctctagtt
      241 atacccctta agtataaagt cccaggtaaa acataatgta cattacattc ttttgcatgt
      301 ctaaatagcg aatttatatt atcaaaatca tctgtaatat atggtataat tggcataata
      361 tgtagaccaa ctgatgcgtt agtttttcta aattctttta acattttaaa tcgtctcatt
      421 gaatccacac catttggctc aataagcctt tgtgtatctt catttactgt ggtaattgta
      481 gatgctacat tgatatatgt aatcctagat agcttatcaa ttaaatcgta atctcttaaa
      541 attaaatctg actttgtaga aataatagct ggtgtcttgt attttattag caggtttaat
      601 atttctggca taattttata gtcagcttct attggttgat aactatctgt aactcctcct
      661 atattgatta cttctttctt ccatttactg cttttaagtt gtctctcaag tttttctact
      721 atatttgttt ttacataaat ctcttcaaaa taattattgg agttgatgta cttatgagag
      781 taaatagcat aacaatattt acatccatgc tcacatccac gataaatatt taaatcccat
      841 ttatatggca tactcctttt taatttatta caagctattt cacaatttat tggaatatat
      901 tttattttat tatacaaata atcacctctt tatatacaat aatactataa tgttaatcta
      961 tagtaattaa aacatgatat ttccagacca caattcataa tctttactat atttacaaag
     1021 taatatattc cattcattta ttggtattcc tccacatgtt gctttttctt ctaaattata
     1081 taagcattgc tttaaataca ttttattatg ccaatcatca tataaatttt ctaaatttaa
     1141 actatgttca ttagatatat catttgcaaa atttctattt ttccatttta atatacttgt
     1201 atatttaagt tcattaggtt tataatttct ttttaacatt tcatcatgta caatttttgt
     1261 ataaaattta aattcatcta tactataatt caatacttta ttcactaata gatggtttgg
     1321 agtacctttt ttatctatag aaccttttat agcaagtagc tctctccatt gacttactaa
     1381 ttgattttta ggtaatacat caattaaatc cttatgccat aatctcataa tttaactcct
     1441 tactataaaa tattttataa attcaataac caatctatct tacaatttta catgttcaat
     1501 aattttatct gtaaaaacta taccatttaa atggtctatc tcatgacaaa gacattttgc
     1561 cataaaacct gaacctttaa tttctatttt ctcaccgtat tcgtttagag cttgcactgt
     1621 aacttttttt ggtcttttta atttacccca tacttcagga aaacttaaac atccttcaac
     1681 aacaatttgt tccccttctt gtttaataat ttcaggatta acaagcttaa taagtccttc
     1741 tcctaaatct ataacaacta atcgctttaa tactcctact tggcatgcag ctaatcctcc
     1801 tccatttgga gtattgtaca tggtctctgc catgtcattt aatatttctc ttatcttatt
     1861 atctacgttt tctacatact tactcttttt tcttaaaatt tcatcatcaa atgttcttat
     1921 ctttcttata gccataatat tctcccctac ttatctgtgc tcttgatttt ctttcacttt
     1981 cttcaaataa aaatacactt tcaattggtt cattaaaaat tcttgaaata tcatatgcca
     2041 accatattga aggttcattt ttaccagttt ctatagcatt tatagcctgt ctagatgttc
     2101 caactaatct ccctagttgt tcttgtgtca atcctaattt ttctcttaat atctttattt
     2161 tatttttcat atttgctcca ttcatgtaag gttaacttta catcttaaat gtaacatttt
     2221 tcaacattac tgtcaagtca accttacatt tatatattaa attttcttat ttaactattt
     2281 ttttactaaa ctttttaaat aataataaaa tccatattaa tgatataaca attgaaataa
     2341 acatatagac ataattatct tccttaaatg ccttcgatat ccaaaaagtt ggtaagaaga
     2401 acagaatata ttgtgcttta tttaaaataa aaaatggtgc taaaataccc atcataaaaa
     2461 cacctgagaa tttcgtaatt accattcctt ctagtttatt tgatgataaa gaaatcacta
     2521 atattgatat aataattcct tgtaataaaa ctaataaaga aattgataag tttaacaaga
     2581 aagaaatttt agttagagaa aaaaagataa gtagaatcat agtaataata aatgctaaga
     2641 tacctggtat tccaaatcgt gaaataagat aaccagtttt accaagcgga gttactatta
     2701 aatatcttga aatgctgtca tcaatttctc ccaatatgac cattgtagaa gcaaaaaaga
     2761 acataaaagg tgtaataaaa gctaaaagta aatcaaacat caagaaatac ggctctaaat
     2821 ttaattgata ataaaacttg ttaagtgata tattctgtat taatggtatt ccaaatttta
     2881 taaatactcc acataatatt ggagaaacac atactataaa aagcatagca tctctcttaa
     2941 tttgttcaaa ctcttgttta aacgcattta taatctttat catagtttta ctccacctac
     3001 ctttttccaa ctgctacaaa taaaataata agttataaaa taaattatta ttataattga
     3061 tattaaaatt aaaatattaa tcattataaa atttttatct ccactaataa gagaaataca
     3121 tatattaaaa gggtacaaat ttgcatatga ttttgtgtca agaagtacat tgagtattgg
     3181 tggaataaag catataatct caataggaat acttaaaaca ataaactgat tcaaactaga
     3241 tgctttactt gcaagtatca atccaagcaa agaaaatata atagaagaaa aaaaggtacc
     3301 tatagttgat attataatat tatctaaatt taatgtaata gcaataaaca tagctacaat
     3361 actagatatt actcctaatg aaattacctt tgataaaata tactcagata cttttactgg
     3421 agatacagct aaggaattta aaaccctttg acttttctca agaagtacaa tagctcccat
     3481 aaaaaacaat cccattgctg ctgggtcaga ataaatcata attatagcta ttttttctct
     3541 cataaaagtt ggaaatacat tgataataca aatatatata atacttaaaa aaagatataa
     3601 aaaataaaaa ccatacttaa attgaaactt tatatctcct aaaataagat tttttagcct
     3661 cattgtaaat tcctccctgt tatctcaata aaaatatcat ttaatgttgg ttcactacta
     3721 tgtattgata aaagtttatt tttctcaata agcatattca aatttttatc attagcagta
     3781 ttgttcagaa aacattcaga agttttttct ccattatcaa agtaagtata tctaacttta
     3841 atagctcctt ttgacataat aagattatgt ggtgtatcta aagcagatat tttaccattt
     3901 acaatgaacg ctactctgtc acaaagctct gttgcatcta acatgttatg agttgttaaa
     3961 ataatagttt tacctttaga tttttctgat aaaattatat ctttcataac cttgctattt
     4021 gatgggtcaa gaccacttgt tggttcatct aaaaataaaa tatctggatt atgtaataaa
     4081 gcttttataa aatttaatcg agatttcata ccttttgaat actcagatac ccttttattt
     4141 gcttcatttt ctagtcctac tgattttaat aactcatcaa tagatagtag ttttttagaa
     4201 tatagagacc caaaatattt taaattttct attgcagtca acttttcata taaactagga
     4261 aattcaaaat caactcctat attttcataa aatttatttg agtgtctttt actctctact
     4321 ccatttacaa tgacacttcc tccataatta gttatcattc caattagaat cttttgtaat
     4381 gtagattttc ctgcacctga tggtcctaaa aatcctagta tttccccttt tcctactcca
     4441 aagttaatat tttgtaaaaa atctctctct gtataactaa atgataaatc atcaacttta
     4501 atcattcaat atcacccttt caaatttttg actattttga ctatatggtc aataaaatat
     4561 aatttttttg tttgttaata atttttaata aaagttatta gcaaacaaaa tttaaattct
     4621 attccataag ttgtataact aatccgttta tcattaatct taaagcatct tcaaaacaat
     4681 tacttccaac ctcgcgctta tatagcatgg taagaaaaac tgctctaaat gctccactaa
     4741 agctttctat gtctttattt tttgcatttg gtatgcaaga tataatctct ccaatttcaa
     4801 agtcatcatg tctaaagtgt tcttcaatta tagaatctgg taactttctc accaatatct
     4861 caaattctcc atttatcata agatttaaaa ttgaagtact atcaaccatc ctatagaatt
     4921 tcataaatat atctgtcaat tgttcacatg taatactttc atctactcta tttatctcat
     4981 ataagatttc tttttgtata gagtcatgta tatctcttaa tacctcaaaa aagagcaatt
     5041 cttttgattg aaaaaataga taaaaagttc cttttggtat ttttacccgt tttacaagct
     5101 catcaacagt agtttttcta ataccatatt gctccataca caatcttgct tcttctttaa
     5161 gtctttttat tatatactct ctttctttat cagaatacga tttaggcatc ttttccctcc
     5221 agtttgacta aaataattta tatagtcaat ataatacttt ttctatctaa tgtcaatagt
     5281 attattagta tatttaaaaa taagcttaaa aactttcgaa aattctgaat tttatata
//
LOCUS       DAMJYR010000206         5332 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.211, whole genome shotgun sequence.
ACCESSION   DAMJYR010000206 DAMJYR010000000
VERSION     DAMJYR010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5332
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.211"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(18..860)
                     /locus_tag="PC471_003644"
     CDS             complement(18..860)
                     /locus_tag="PC471_003644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDF2790726.1"
                     /translation="MIIQQIYNNNVVLVLDENIKKELILTGCGIGFQKKKGQEIDKSK
                     IERTFVIQDESFLDKISKLASQVDEKFFEVSTEIIAYAEENLNTKLYEYIYVALTDHI
                     AFAIKRYHENITIKNDLLHEIKRIHKKEYEIGKWAVDYINKEFDVKFPVDEAGFIAMH
                     IVNSNYKGSSKESLLITKIVKDILNIIRYYYRVEFKEDDINYDRLLTHLKFFAKRLVK
                     KEKINDTNNEIIDIIKVKYEKDYDCAYKIKTHVEKNYDYYVSQDELLYLTLHIKRVIS
                     VLNL"
     gene            complement(1121..3163)
                     /locus_tag="PC471_003645"
     CDS             complement(1121..3163)
                     /locus_tag="PC471_003645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HDF2790727.1"
                     /translation="MSILVTLLGLLVCTTISTVFSKKWSNIPLAIYQIVLGIILSILP
                     FKFSFSFNPEIFVICIIAPLLFSEGQNVSRKELLELRKPILLLAFGLVLITVFAGGIF
                     IHFLIPGMPLSVSFALAAVISPTDLVAVKSITQGLNFPKNMMSILEGESLLNDAAGVV
                     AFKVAVLATVTGVFSIEEAGIQFMITAFGGIIVGSILGYIIIKIRLSLHKWNLEEIPM
                     VIVIQIMTPLFVYFVAEEIGVSGILAVVMAGIAHGIEKEHLQNTTTKLRIISDNTWYV
                     LEYVLNGFVFTLLGFLLPSIYTGLSSKNENMALELTFISTLIVLILFVIRFIWVYLWH
                     NSFIKPKKNPLNNFFVGFLGFKDEEVVKESISKCKYSLIVATCGVHGTFTLATALSIP
                     FYLADKDVFPMRDTVLFISSEVILISLVLATVLLPRLLKNNVQESEPLLSDKEAYKLI
                     LKEAILKLSNEKAQEVQPVIHDLNEQLVDSEKGILNTPDNKLISMMIAEADQQGLLAV
                     LKLLEDGKISDRAFKLYRFYITKSRKSVQKSIFKIVKLKISMWIINRKVKKEVKEKIT
                     KIVSENKEIIKEFHHAYALSTKTAISFINKNTDEDNRHEALIAIRFYNRYLNWITRKI
                     QNDDKFEARVEYYRVMAIQSQRDSVQQLVETKQISREVAKELLENILYDEMMMIES"
     gene            complement(3315..4286)
                     /locus_tag="PC471_003646"
     CDS             complement(3315..4286)
                     /locus_tag="PC471_003646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HDF2790728.1"
                     /translation="MKKEESLLKVEGLKKYFEVKSGLFDKEIKCVKAVDGISFDLKKG
                     ETLAIVGESGCGKSTAGRTILRLIEKTEGKVEFNGVDIYKLEKEELRKLRPKMQMIFQ
                     DPYSSLNPRLNVEQIISEAVIEHNLVPKNEIKDNILNVVESCGLSKYHLNRKPGEFSG
                     GQRQRIGIARALALNPEFIVCDEPVSALDVSIQSQIINLLMDLQDKYKLSYLFISHDL
                     SVVNHIAHRVCVMYLGSIIEIATKGELYLHSQHPYTKALMSAVPIQDPTIKKNRIILK
                     GDIPSPTNPPSGCKFHTRCPYCKEICKKEIPVLKEISRNHFVACHLV"
     gene            complement(4287..5264)
                     /locus_tag="PC471_003647"
     CDS             complement(4287..5264)
                     /locus_tag="PC471_003647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2790729.1"
                     /translation="MSKALIEVKDLKVYFHTDKGIVKSVNEVSFNINEGETIGIVGES
                     GCGKSVTAMSLMKLLPTSKIEGGEIIFRGKDILKMNEDELMGIRGNEISMIFQEPMTS
                     LNPAFTIGSQIIEGIMIHQDLSKEEAKKKVIDMIKLVEIPRAEEIYNSYPHELSGGMR
                     QRIMIAMALSCNPKLLIADEPTTALDVTIQAQILDIMKNIKEKLNTSIMMITHDLGVV
                     AEMCDKVLVMYAGKIIEVAEVVELFKNPKHPYTIGLLKSKPALGKNKDKRLYSIPGQV
                     PNPIGMPDSCYFSDRCEKVCDKCRTQIPPLIELNSGHSIACWLYKKEEI"
BASE COUNT         1731 a          926 c          548 g         2127 t
ORIGIN      
        1 tattaaacta ctctttatta taaattcagt acagatataa ctcttttgat atgtaatgtt
       61 aaataaagta gttcatcttg acttacatag taatcataat tcttttctac atgagttttt
      121 attttatatg cacaatcata atccttttca tactttactt taattatatc tataatttca
      181 ttatttgtat cattgatttt ttcttttttt actaatcgtt ttgcaaagaa ttttaaatga
      241 gtcagaagcc tatcataatt tatatcatct tctttaaatt caactctata ataatatctt
      301 ataatattta gtatgtcttt tacaatttta gtaattaaca gtgattcctt tgaacttccc
      361 ttatagtttg aatttactat atgcattgct ataaaaccag cttcatctac tggaaatttc
      421 acatcaaatt ctttatttat ataatctaca gcccatttcc caatctcata ttctttttta
      481 tgtattcttt ttatctcatg tagtaaatca ttctttatag ttatgttttc atgatatctt
      541 ttaattgcaa atgcaatatg gtcagtaagt gctacataaa tatattcata aagtttagta
      601 ttcaaattct cctctgcata agcaattatt tcagtactaa cctcaaaaaa tttctcatca
      661 acctggcttg ctagtttact aattttatct aaaaagcttt catcttgtat tacaaaagtt
      721 ctttcaattt tacttttatc tatttcttga cctttctttt tttgaaaccc aattccacat
      781 cctgttaata ttaattcttt ttttatattt tcatctaaaa ctaatacaac attattatta
      841 taaatttgtt gaataatcat aatcaatatc tctcccaaaa tacaatttcc cttattcata
      901 aaaaaatacc taaattaaga tacaattcac ctgtatacct taacctaggt attgcctgct
      961 taccagtaac aatccagctt gttaacgctt atatattgta ttcaatttat ggttttattc
     1021 tattataaat aaatatatat gtcaagtaaa ttattgaaaa ctatttccta ttttttagta
     1081 ttaattttaa catcaaaaat caaattttat tccattatat ttatgattct atcatcatca
     1141 tttcatcata gagaatattt tccaataact ctttagccac ttctcttgat atttgcttag
     1201 tttcaactaa ttgttgaaca ctgtctcttt gactttgtat cgccattact ctgtagtatt
     1261 ctactcttgc ctcaaactta tcatcatttt gaatctttct agtaatccaa ttcaaataac
     1321 gattatagaa tctgattgct attaaggcct catgtctgtt atcttcatca gtatttttat
     1381 ttataaatga tatagcagtt ttagtagaaa gtgcatatgc atggtgaaac tctttgatta
     1441 tttctttatt ctcagatact atttttgtaa ttttttcttt tacttctttc tttacttttc
     1501 tatttataat ccacatgctt atttttaatt ttacaatttt aaatatagat ttttgaacag
     1561 attttcttga ttttgtaata taaaatctat acaatttaaa ggctctatca gaaattttac
     1621 catcttctaa tagtttcaaa acagctaata atccctgttg gtctgcttct gcaatcatca
     1681 tacttataag tttattatct ggagtattta gtattccttt ttctgagtct accaactgtt
     1741 catttaaatc atgtataact ggttgtactt cttgtgcttt ttcattacta agttttaaaa
     1801 tggcttcttt caaaattagt ttatatgcct ctttatcaga taataaaggt tctgactctt
     1861 gtacgttatt ttttaataat cttggcagta aaactgttgc caatactaaa cttataagaa
     1921 taacttctga tgatataaat aaaacagtat ctctcattgg aaatacatct ttatcagcca
     1981 aataaaaagg tatagataag gcagttgcta gtgtaaatgt accatgaaca ccacaagtag
     2041 caactattag tgagtattta catttagaaa tactctcttt tacaacttct tcatccttaa
     2101 aacctaaaaa acctacaaaa aaattattta aaggattctt tttaggtttt ataaaagaat
     2161 tatgccataa atatacccat ataaatctta taacaaataa aataagtaca attaaagtag
     2221 aaataaaagt caattccaat gccatatttt catttttact actaagtcct gtatatatac
     2281 ttggtaacaa aaaaccaagc agtgtaaaga caaatccatt caatacatac tccaaaacat
     2341 accaagtatt gtctgatatt atccttaatt tagtagttgt attttgtaaa tgctcttttt
     2401 caattccatg agctatccct gccattacaa cagctaggat tccagatact cctatttctt
     2461 ctgctacaaa atatacaaaa agtggagtca ttatttgtat aactattacc attggaattt
     2521 cttccaaatt ccacttgtgt aaactaagtc ttatttttat aattatgtat cctaaaatac
     2581 ttcctactat tattcctcca aaagctgtaa tcataaattg aatccctgcc tcttctattg
     2641 aaaaaactcc tgtaactgtg gcaagtactg caaccttaaa tgctactact ccagcagcat
     2701 catttaataa agattcacct tcaagtatac tcatcatatt tttaggaaaa tttaatcctt
     2761 gtgtaattga ttttactgct actaaatcag ttggagatat tactgctgcc aaagcaaatg
     2821 aaacagataa tggcatccca ggaataagaa aatgtataaa gattccacct gcaaacacgg
     2881 ttattaaaac taaaccaaat gcaagaagca aaattggttt tctgagctct aatagctctt
     2941 ttctcgatac attttgtcct tcactgaata gtaatggagc tatgatacat attacaaata
     3001 tttctggatt aaatgaaaat gagaatttaa atggcaaaat agacaatata atccctagta
     3061 caatttgata tattgcaaga ggaatattac tccacttttt gctaaataca gttgaaatcg
     3121 ttgtacaaac caataatccc aacaatgtta ctaaaattga cataactacc tctcttttca
     3181 ataataatat tatagattaa acttatatat caactaactt aatattaaca gcatgaaatc
     3241 ccatcttaat caaaaaaatt ttcagtttat tctattattc ttattatata catcaaattt
     3301 aatttttgaa atatctatac tagatgacat gcaacaaaat gatttcttga tatttctttt
     3361 aaaactggaa tctctttttt gcatatttcc ttacaataag ggcatcttgt atgaaactta
     3421 catccacttg gtggatttgt tggagatggt atgtctcctt ttaatataat tctatttttc
     3481 ttaattgtag ggtcttgtat tggtactgcg ctcataagcg cttttgtata tggatgttga
     3541 gaatgtaagt aaagttctcc tttagttgca atttctatta tagaacctag atacataaca
     3601 caaactctat gtgcaatatg atttactaca ctcaaatcat gagatataaa taaatatgaa
     3661 agtttatatt tatcctgtaa atccataagt aaatttataa tttgagattg aatagaaaca
     3721 tctagtgcag aaacaggttc atcacaaact ataaattctg gatttaatgc taatgctctt
     3781 gctataccta ttctctgtct ttgacctcca gaaaactcac ctggttttct atttaaatgg
     3841 tacttagaaa gaccacaact ttctacaaca tttaatatat tatcttttat ttcattcttt
     3901 ggcactaaat tatgttctat aactgcctcg gatattattt gttccacatt taatcttgga
     3961 tttaatgaac tatatgggtc ttgaaatatc atttgcattt tgggtcttaa ctttctaagt
     4021 tcttccttct ctaacttata tatatctaca ccattaaatt caactttacc ttcagttttt
     4081 tcaattaatc taagaatggt tcttcctgca gttgattttc cacatcccga ttctccaact
     4141 atagccaaag tttctccttt ttttaaatca aaagaaattc catctactgc ctttacacat
     4201 ttaatttcct tatcaaataa tccagattta acttcaaagt actttttaag accttctact
     4261 tttaataaac tctcttcttt tttcaattat atctcctcct ttttatataa ccaacatgca
     4321 atactatgac cactattcaa ttctattaaa ggaggtatct gagttctaca tttatcacat
     4381 actttttcac atctatcact aaaataacat gaatctggca tcccaattgg atttggtact
     4441 tgtcctggaa tcgaatacaa tcttttatct ttgttttttc caagagcagg ctttgatttt
     4501 agaagtccaa tagtataagg atgttttgga tttttaaata attcaacaac ctctgccact
     4561 tctataattt tgccagcata catcactaat actttgtcac acatttctgc aactactccc
     4621 aaatcatgtg taatcatcat tatagatgtg tttaattttt cttttatatt tttcattata
     4681 tctaaaatct gagcttgaat agtaacatct aaagctgttg ttggttcatc agctataagt
     4741 aattttggat tacatgataa agccatagct atcattattc gttgtctcat tccacctgat
     4801 aattcatgtg gatatgaatt atatatttct tcagctcttg gtatttctac cagttttatc
     4861 atatcgatta cctttttctt agcttcttcc tttgataaat cttgatgtat cataatacct
     4921 tcaatgattt gacttccaat agtaaacgct gggtttaaag atgtcattgg ttcttgaaaa
     4981 atcattgata tctcattccc tcttattccc ataagttcat cttcattcat ctttaaaata
     5041 tctttgcctc gaaagattat ttctccacct tctatttttg atgttggaag taacttcatc
     5101 aaagacatgg ctgttacaga cttaccacat cctgattctc caactattcc aatagtttca
     5161 ccttcattta tattaaaact tacttcattt acactcttta caatcccttt atctgtatga
     5221 aaataaactt ttaaatcttt aacctcaatt agtgctttac tcattatttt ctcctctcag
     5281 caaaattact taagataaca tacacctttt gattgtattt tataaaaatt ta
//
LOCUS       DAMJYR010000207         5293 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.212, whole genome shotgun sequence.
ACCESSION   DAMJYR010000207 DAMJYR010000000
VERSION     DAMJYR010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5293)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5293)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5293
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.212"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            99..935
                     /locus_tag="PC471_003648"
     CDS             99..935
                     /locus_tag="PC471_003648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDF2790730.1"
                     /translation="MNIKKIFNNNVVVSSLEDGTEIIVTGAGVGFKKKVGDLIDENLI
                     SKKYFVQDDQRDKYNQILNKTSIEYFKISEEIIEKANEVLNTQVNDSIILALTSHIEF
                     AVQREKQGIKLPNLILNETKQLYREEFEFGLWAIDEIEKRIGIKLPEDEAGYIAIHII
                     NGLENSQKDEGINILEFSKQVIQIIEEVHGFKLDVNSLNYTRLITHLKFFVQRILRKE
                     TYNDSDIEDMYKLVNKNYNKPKVCTEEIAALVLDRFEYKISKEEELYLMIHINKITNS
                     GC"
     gene            1288..2865
                     /locus_tag="PC471_003649"
     CDS             1288..2865
                     /locus_tag="PC471_003649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDF2790731.1"
                     /translation="MKKNKLGLWNFFQMLGKTFMFPIALLSVSGMMLGLGAGFTDPKM
                     IEMVPFLGNEYVNLVLNFMLTIGLFAFNNLGALFAMAIPLGLLKKEKEFGAFSGLVGF
                     IAMHIGTNFYLTRADLLVPAEQMSTNGQAMIMGIQTYNTSVLGGIVAGLIVYAIYDKV
                     SNLKIPESLGFYSGPRLVPIVSLIVMSIVGLLIPIVWPPFFNAFQSLGRWISSAGPLG
                     YFSYAVAERVTIPFGLNHLVTSVFRFTPIGGTAVIDGETYFGTLNMFMAYVENNQIIP
                     LDLAGKMEQGKLMIQYGLAGAALAIYRTAKPENRKALKGLLISGVLTVIIGGISEPIE
                     FLFLFISPALFAFHTFMNGLANMVLPYLGVLMGFTGDLIAFISFGVLRGTATGWPIAV
                     LVAVLYFAIYYFVFKWAIIKFNIKTPGREDKEIKVSEGNGSNFKNLSTYKGQQMISAL
                     GGQSNILSLDNCVTRLRLKLQDVSLINEDAIKEAGGIAVVKLDEHTIQVIIGTQVYSL
                     RKQMDKAMEGHLEACND"
     gene            2858..4042
                     /locus_tag="PC471_003650"
     CDS             2858..4042
                     /locus_tag="PC471_003650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PatB family C-S lyase"
                     /protein_id="HDF2790732.1"
                     /translation="MINFDKKVDRLGTYCTQWDYVEDRFGKKDLLPFTISDMDLEFPF
                     EITEVLQERLNHRVLGYSRWKHDDFRNSVTNWYIKRFNCSVDSNWIYYSPSVMYSISK
                     LIEMLSNEGDGILINTPAYNAFYDVIINNNRNIIKSHLVNRNGFYSIDFNDFEKKCKK
                     SKIFILCNPHNPTGRVWSELELVKMIGICKNNDVKIISDDIHMDIVYDNRMTPILNVA
                     KDYLDSVFICTSASKSFNIPALTGSYVIIPNEGIRNKFENITRYRDFVNSPAILAILA
                     TMVCYDDCEYWLDELLKYLKENLKYTIDYIKDNLEPLKLVMPEGCYFAWIDFSELKIS
                     GEKFQKLLMDIGEVAIMSGSVYGEEGEFYLRLNVACSREKLEDGLNRIKKTMDYIEKR
                     IL"
     gene            4060..4659
                     /locus_tag="PC471_003651"
     CDS             4060..4659
                     /locus_tag="PC471_003651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HDF2790733.1"
                     /translation="MGYLKTMVDVMTTEMNAIRCLIDEAGIEYESIVSEIANCEGKVI
                     FMGVGKSAHIGKKLAATFASTGTPSFFVHATEAVHGDLGMIESKDITILISNSGNSME
                     VVNCIKYIKAIGSKTIAFTSNRNSVLAKECDYALIYPAKDEADHLNLAPTTSSTITLV
                     LGDSIACALSKSSNFGSSDFYKYHPGGSLGEKLKTANNG"
     gene            4683..5153
                     /locus_tag="PC471_003652"
     CDS             4683..5153
                     /locus_tag="PC471_003652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDF2790734.1"
                     /translation="MFKMFKKKSIKSPISGKVIELSKIQDGVFSQKIMGEGVAIDSTG
                     DIVYAPINGKIVVVAETKHAFVIELENGMELLIHVGLDTVNLKGEGFEALVSMGEQVK
                     EGTPMLKINRPLIESKGISLITPVTITNHSEYNMNTCNVGNSVEGGKDTVIEFK"
BASE COUNT         1977 a          559 c         1017 g         1740 t
ORIGIN      
        1 aaaatatata cgaaagtaaa attttaaata ttgacattaa attaaatata ctatactatg
       61 taagtgtgat gagagaattt ttttagaggg gatttatagt gaatataaaa aagatattta
      121 ataacaatgt agtagtaagc tccctagaag atggcacaga aataattgtc actggtgctg
      181 gagtaggatt taagaaaaaa gtaggcgatt tgatagatga aaatttgatt tctaagaaat
      241 attttgttca agacgatcaa agggacaaat acaatcaaat acttaataag acctctattg
      301 aatactttaa aatttcagaa gaaattatag aaaaggctaa tgaagtttta aatactcaag
      361 tgaatgattc aataatatta gccttaacta gtcacataga atttgctgtc caaagagaaa
      421 aacaaggtat aaaattacca aacttaatat tgaatgagac aaaacaactt tatagggaag
      481 aatttgaatt tggattatgg gcaattgatg aaattgaaaa aaggatagga ataaaattgc
      541 ctgaagatga agcaggatat attgctattc acataatcaa tggtttggaa aattctcaaa
      601 aagatgaagg tattaatata ctagagtttt caaaacaagt tattcagata attgaagaag
      661 tacatggatt taaattggat gtaaattcat taaattatac tagacttata actcacttaa
      721 agttttttgt tcaaaggatt ctccgaaaag aaacttacaa tgatagtgat attgaagata
      781 tgtataagtt agtgaataaa aattacaata aacctaaagt gtgtactgaa gaaattgcag
      841 cattggtatt agacagattt gaatataaga ttagtaaaga agaagaatta tatttaatga
      901 ttcacataaa taaaataaca aacagtggtt gttaggattg taactgataa agcaggcaaa
      961 acctaagtca ttgagcttaa ctattagatt aagttttttg atttaggttt ttttattaga
     1021 tacaatcaaa atacagacag tgacttttag gattgtaact ggttaagcag gcaaaaccta
     1081 aatcattgaa tttaactatt aggttaagtt ttttgattta ggttttttta ttagatacaa
     1141 tcaaatacag acagtggttg ttaggattgt aactggttga gcaggcaaaa cctaaatcat
     1201 taaacttaat tattaaatta agttttttga tttaggtttt tctataaatt tgattatgtt
     1261 tttagataaa aataggaggg gaaaattatg aaaaaaaata agttaggttt atggaacttc
     1321 ttccaaatgt taggaaaaac gtttatgttc ccaatagcac tacttagtgt ttctgggatg
     1381 atgctaggac ttggggcagg ttttacagac cctaagatga ttgaaatggt tccattttta
     1441 gggaatgaat atgttaactt agttttaaac ttcatgttaa caataggatt atttgcattt
     1501 aacaacttgg gagcattatt tgcaatggca attccactag gattattaaa aaaagaaaaa
     1561 gagtttggtg ctttttcagg attagtagga tttatagcaa tgcatatagg tacaaatttt
     1621 tatttaacaa gagctgattt gttagtacca gcagaacaga tgtcaactaa tggtcaagct
     1681 atgattatgg gtatacaaac atataataca agtgttttag gtggtattgt agcaggatta
     1741 attgtttatg caatatatga taaagttagt aatttaaaga taccagaatc attaggattc
     1801 tacagtggac caagattagt tccaattgtt agtttaatag ttatgagtat agttggatta
     1861 ttaataccta ttgtttggcc accatttttc aatgcattcc aaagtttagg aagatggatt
     1921 tcttcagcag gaccattagg atatttttca tatgcagtgg cagaaagagt tacaattcca
     1981 tttggactaa atcatcttgt aacatctgta ttccgtttta caccaatagg tggaactgct
     2041 gttattgatg gagaaacgta ctttggtaca ttgaacatgt ttatggcata tgtagaaaat
     2101 aatcagatta tacctcttga tttagctggt aaaatggaac aaggtaaatt gatgattcaa
     2161 tatggattag ctggtgctgc attggctatc taccgtacag caaaaccaga aaatagaaag
     2221 gcacttaaag gacttttaat atcaggagtg ttgacagtta ttataggtgg tataagtgaa
     2281 ccaatagagt ttctattctt attcatatca ccagcattat ttgctttcca tacatttatg
     2341 aatggtctag caaacatggt acttccatac ttaggcgtgt tgatgggatt cacaggagat
     2401 ttaattgcat ttataagttt tggtgtgtta agaggaacag ctacaggatg gccaatagca
     2461 gtattagtag cagttttata ttttgcaata tactattttg tatttaagtg ggcaattatt
     2521 aagtttaata taaagacccc aggacgtgag gataaagaaa taaaagtatc tgaaggaaat
     2581 ggttcaaatt tcaaaaattt gtctacatat aaaggacaac aaatgatatc tgctttaggt
     2641 ggtcaatcta atatattatc actagataat tgtgtaacta gattacgttt aaaattacaa
     2701 gatgtatcat taataaatga ggatgcaatt aaagaggctg gtggtatagc agttgttaaa
     2761 ttggatgagc atacaataca agtaattata ggaacccaag tgtattcttt aagaaagcag
     2821 atggataaag ctatggaagg tcatttggag gcatgtaatg attaattttg ataaaaaagt
     2881 agaccgattg ggtacatatt gtacccagtg ggactatgta gaagatagat ttggtaagaa
     2941 ggatttgctt ccttttacaa tttcagatat ggatttagag tttccatttg aaataacaga
     3001 ggtgttgcag gaaaggttaa atcatagagt tttgggatac agtagatgga aacatgatga
     3061 ttttagaaat tcagttacga attggtatat taaaagattt aattgtagcg ttgatagtaa
     3121 ctggatatat tatagtccaa gtgtaatgta ttcaattagc aaacttattg agatgttaag
     3181 taatgaagga gatggaatcc ttataaatac tcctgcatac aatgcttttt atgatgtgat
     3241 aataaataac aatcgtaata ttataaagtc acatcttgta aataggaatg gattctattc
     3301 aattgatttt aatgattttg agaaaaagtg taaaaaatct aagatattta ttttatgtaa
     3361 tcctcacaat ccaactggta gagtatggag tgagttagaa cttgttaaga tgataggaat
     3421 ttgtaagaat aatgatgtca agataatatc agatgatata cacatggata ttgtatatga
     3481 caatagaatg acacctatct taaatgttgc aaaggactat ttagattctg tattcatttg
     3541 tacatcagcg tctaaatctt tcaatatacc agcattgact gggtcatatg tgattatacc
     3601 aaatgaaggt attagaaata aatttgaaaa tatcactaga taccgtgatt ttgtaaattc
     3661 gccagcaatt ctagcaatat tagccacaat ggtctgttat gatgactgcg aatattggtt
     3721 agatgaacta ttaaaatacc ttaaagagaa tcttaaatat acgattgatt atataaaaga
     3781 taatttagaa ccattgaaat tggtcatgcc agaaggctgt tactttgctt ggatagattt
     3841 ttccgaactt aaaataagtg gtgagaaatt ccaaaaatta ttgatggata ttggagaagt
     3901 tgctattatg tcggggtctg tatatggtga agaaggtgag ttttatttaa ggttaaatgt
     3961 ggcatgttca agagaaaaac ttgaagatgg tttaaatagg attaaaaaaa ctatggatta
     4021 tattgaaaaa agaattttat agtaggaagg gagatagtta tgggatattt aaaaacaatg
     4081 gttgatgtta tgactactga aatgaatgcc ataagatgtt taatagatga agcaggaata
     4141 gaatatgaaa gtatagttag tgaaattgca aattgtgaag gcaaagtaat ttttatgggt
     4201 gtaggaaaat ctgcacatat aggtaagaaa ttggcagcta catttgctag tactggtaca
     4261 cctagctttt ttgtacatgc aacagaggca gtgcatggag atttaggaat gattgaaagt
     4321 aaggatatta caattttaat atcaaatagt ggaaattcta tggaagtggt aaactgtata
     4381 aaatatatta aagcaatagg ttctaagaca attgcattta cttcaaacag aaattcagta
     4441 ctggcaaaag agtgtgatta tgctttaata tatcctgcaa aggatgaagc agaccattta
     4501 aatctagcac caacaacatc atctacaatt actttggttt taggagattc tattgcatgt
     4561 gcattatcta agagtagtaa ttttggttct tcagattttt ataagtacca tccaggagga
     4621 agtttaggtg agaagttaaa aacagctaac aatggataaa agcaaaaagg agagtagttt
     4681 ttatgttcaa aatgtttaaa aaaaaatcaa taaaatcacc catatcagga aaagtaatag
     4741 agttatctaa aatacaagat ggtgtatttt ctcaaaaaat tatgggagag ggtgtagcta
     4801 tagattctac aggagacatt gtatatgctc caatcaatgg aaagatagta gtagtagcag
     4861 agactaaaca tgcatttgta atagaattag aaaatggaat ggaacttctt attcatgttg
     4921 gtttagatac tgtaaattta aaaggcgaag gttttgaggc attggtgagt atgggagaac
     4981 aagtgaagga aggaacacca atgttgaaaa ttaaccgtcc tttgattgaa tcaaagggta
     5041 tatcacttat tactccagtt acaataacta atcattcaga gtacaatatg aatacatgta
     5101 atgtaggcaa tagtgttgaa ggtgggaaag atactgtaat tgagtttaaa taaagaacta
     5161 aaatacaagg ttaatattta taaataccta taaacttatg ttttataaga ttgattgtat
     5221 agatttgttg tataaaaaag aactctttaa ttataaatta aggagttctt ttttattttg
     5281 taaatattgt tta
//
LOCUS       DAMJYR010000208         5242 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.213, whole genome shotgun sequence.
ACCESSION   DAMJYR010000208 DAMJYR010000000
VERSION     DAMJYR010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5242)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5242)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5242
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.213"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            187..1098
                     /locus_tag="PC471_003653"
     CDS             187..1098
                     /locus_tag="PC471_003653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CGNR zinc finger domain-containing protein"
                     /protein_id="HDF2790735.1"
                     /translation="MNKKIELIGNFASDWIRYSDYEYKITEEDELYIVPTEDAVFTMY
                     NPFDVADDIIVDIIRIGQEALQDISKETKEMKLKKEVLEFVKKYGLLGLIYSSVYNRD
                     IVGEERVLMIEKNYITKENILSTDEYVNMFIPFASDEDVYFKKYRRGIDVSKREESPK
                     FFGKRPLILDLVFSKFYTEQLDWIVSFAKDMCKHFNQICVYKNTSNLLTESVTIMSGG
                     FNPQKIGFSISQLDKPIIAWEFDSLKTAIETIYAFAITSEPSSLNKCKYCGKIYIPTN
                     SKSQYCSPSCRNCSNVKKSRSRAKKDK"
     gene            1358..1792
                     /locus_tag="PC471_003654"
     CDS             1358..1792
                     /locus_tag="PC471_003654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2790736.1"
                     /translation="MISPIILSSINQNLKEIERNELLETNIESGDYGLALSESDVKDI
                     INSRDNTLKGYGRIELDIKVTKQLIENIYTSQYTNVDNYLEAINDMQEIFYYLKNETD
                     DKICDDEIIEILGEFYEKFSGNMDNVRGEADEFAKKFKFGEV"
     gene            1815..3350
                     /locus_tag="PC471_003655"
     CDS             1815..3350
                     /locus_tag="PC471_003655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2790737.1"
                     /translation="MLNNLSNININENNLIKNQYSISLLKECLNCKVIDEREVYNIQQ
                     EVSLILMDLIKKYTNGQSTSVKTEVAEKLLISIWYAIDAYINKLGDIEKRVVELTAKN
                     VKKVYLGGIEILEEELVRLKDFYKLMMSRKLDTNSIAYNDTLMELSSFFKSYIVKFEA
                     QDVPVSIDYPLALDDMDVKGIYYVKNYIESIDIENRFCNLFKKKDREKLFYDYGMTYK
                     IDCDVILVNLFELIMNNCIFSTILGNEVTNLEISKYEYEFLEKQFKNMYNNITEDKVI
                     DSEESNIIELIEVDDSFNTEVDSNLDYNDFEEINREIRINKKITLLLMEAIEVLIKRL
                     SIEDEDVIRYIKKYEKIFIESVLSSIKSNTFKSMVTVSKNKKEHLEDYIVDDESKLDD
                     ESFREIFNEILDSTSIIEKIRIIKENINAKKDFIDILESECLFGEEYLMLFASLSELE
                     LAILGSVVFYEDIRMREINILEFMLNEKTETTLWKIEYIKFMKRQSEDKIYSIEEHMN
                     KIN"
     gene            3434..4801
                     /locus_tag="PC471_003656"
     CDS             3434..4801
                     /locus_tag="PC471_003656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HDF2790738.1"
                     /translation="MKEYIINNVEEIREELIDLSKKIWENPELAFEEKYASSIQKEYL
                     KSKGFKIEEVENLPTGFIASFGEGKPVIGILGEYDALPELSQCVSAERKPLVEGKAGH
                     GCGHNLLGVAGVGAVVSIKKLIEENKFNGTIKYFGCPAEEEGGGKTVMCINGCFDDVD
                     CAFTWHPFDINAPWRGGSLANLSVKFKFKGITAHAAQAPHNGRSALDAVEIMNVGANY
                     LREHVIDSIRMHYVITNGGGRPNVVPGFAESWYFIRGKKAKDAEHVLDRLIKVAQGAA
                     MMTETEMEYKVTDGIYDYIPNQCLTDLVYDNMVFVGCPKNTPEEEEFAKKLCDTLTRE
                     ERLGVATSLQNDKSIVESYIHKEIVDGDKDKGLAGSTDVGDVSYVIPVAQFAMAAWPV
                     GIASHTWQSCSSAGSNIGFSAMINSAKVLACSAYDVFMDTKIIDEAKIEFDKSLDGQK
                     FKPLV"
     gene            4836..5060
                     /locus_tag="PC471_003657"
     CDS             4836..5060
                     /locus_tag="PC471_003657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HDF2790739.1"
                     /translation="MREKVEKVLEEKIKPVLQRDGGDVELIDVNENGVVLVRLQGACS
                     GCPGVTMTIKAIIENVLVSEVPGVTQVLGV"
BASE COUNT         2165 a          444 c          991 g         1642 t
ORIGIN      
        1 taattatgaa ttagtcatca ataaacaaga aatttagctg taaaaatcca ttataaaggc
       61 tattataaat tttaatatgc taaaaatata aaaagaatag ttgacagtgt agaaaatatg
      121 gaataaacta aaagtgtaat agtaatatga agttatatat tacattgaaa ggtgtgaatg
      181 attattatga ataaaaaaat agaattaata gggaattttg caagtgattg gattaggtat
      241 agtgattatg aatataaaat tactgaagaa gatgaattat atatagttcc aacagaagat
      301 gctgtgttta ccatgtataa cccatttgat gtagcagatg atataatagt agatataatt
      361 cgcataggac aagaggcatt acaagatata tcaaaagaga caaaagaaat gaaactaaag
      421 aaagaagtac ttgaatttgt aaaaaagtat ggattattgg gtttaatata ttcaagtgta
      481 tataatagag atatagttgg agaagaaaga gtactgatga ttgaaaagaa ttatattaca
      541 aaagagaata tattgagtac agatgaatat gtaaacatgt ttataccatt tgcaagtgat
      601 gaagatgtgt attttaaaaa gtatagaagg ggtatagatg tatcaaagag agaagaatct
      661 ccaaagtttt ttggaaagag accattgatt ttagatttag tattttcaaa gttctacact
      721 gaacaactag attggattgt aagttttgct aaagatatgt gtaagcattt taatcaaatt
      781 tgtgtatata aaaatacatc taatctatta actgaaagtg ttactattat gtctggaggg
      841 tttaatcctc aaaagatagg attttctata agccaattag ataaaccaat tattgcatgg
      901 gagtttgatt ctttgaaaac agctattgag actatctatg cctttgcaat tacaagtgaa
      961 ccttcttctt taaataaatg caagtactgt ggtaaaattt atattcctac aaattctaaa
     1021 tctcaatact gttcaccttc ttgtagaaat tgctcaaatg taaagaaaag tagaagtaga
     1081 gccaaaaaag ataaataaaa ttaataaata tatcattgtt tagattttag taaataacta
     1141 ctgaattaaa agttgttata gaaaattata tatctaaatg atggtatttt tttgttgaga
     1201 aaattgaaaa gtttaattga gatagtaatt ttgagttgac aaaaaccact tttaacacta
     1261 ctattataag tggttggtaa aataatatac attaatttaa gtatagcata aaaaacaaaa
     1321 aaagtcaatg gaaaaaataa aaaaggagaa tttaaaaatg atatcaccta ttattttgag
     1381 ttctataaat cagaatttaa aggaaataga aagaaatgaa ctgcttgaaa ctaatataga
     1441 atctggagat tatggtctag ctctttctga atctgatgta aaagatataa taaattcgag
     1501 agataatact ttaaaggggt atggaagaat agaattagat ataaaagtta caaaacagtt
     1561 aatagaaaat atatatacat cacaatatac aaatgtggac aattatttag aggctattaa
     1621 tgatatgcaa gaaatttttt attatttaaa aaatgaaact gatgacaaga tatgtgatga
     1681 tgagattata gaaatactgg gtgaatttta tgaaaaattt tcaggcaata tggacaatgt
     1741 aagaggagaa gcagacgaat ttgcaaagaa atttaaattt ggagaagttt aatatatgat
     1801 aggagagatt ttgtatgtta aataatttaa gtaatattaa tataaacgaa aataatttaa
     1861 taaagaacca atattccata tctctactta aggaatgtct aaattgtaaa gtgatagatg
     1921 aaagagaagt atataatata caacaagaag tatctctaat acttatggac ttgattaaaa
     1981 aatatacaaa tggtcaaagc acatcagtaa aaacagaagt tgccgaaaaa ctgttgattt
     2041 caatatggta tgctattgat gcatatataa ataagcttgg agatatagaa aaaagagttg
     2101 tggagttgac tgctaagaat gttaaaaaag tgtaccttgg aggcattgaa atattagagg
     2161 aagagttagt taggctaaaa gatttttata aactaatgat gagtagaaaa ttggatacta
     2221 attcaatagc atataatgat accttgatgg agttatcaag tttctttaaa agttatattg
     2281 taaaatttga agctcaggac gttccagtaa gtatagatta tccattggca ctagatgata
     2341 tggatgttaa gggtatttat tatgtgaaga attatattga gagtatagat atagaaaata
     2401 gattttgtaa cctatttaag aaaaaagata gagaaaaatt attttatgac tatgggatga
     2461 cttataaaat agattgtgat gttatacttg taaatttatt tgaacttatt atgaataatt
     2521 gtattttttc tactatttta ggaaatgaag tgactaattt agaaatttca aaatatgagt
     2581 atgaattttt agaaaaacaa tttaaaaata tgtacaataa tattactgaa gataaagtta
     2641 tagactctga agaaagtaat ataattgaat tgattgaagt agatgatagt tttaacactg
     2701 aagtagatag taatttggat tataatgatt ttgaagaaat taacagagaa attagaataa
     2761 ataaaaaaat aactttacta cttatggaag caattgaagt attaataaaa agattaagca
     2821 tagaagatga agatgtaata agatatatta aaaaatatga aaagatattt attgaatctg
     2881 tgctaagctc aataaaaagt aacactttta aaagtatggt aactgtgagt aaaaataaaa
     2941 aggaacactt agaagattat attgttgatg atgaaagtaa attggatgat gaatcgttta
     3001 gagagatatt caatgagata ttagatagta catctataat tgaaaaaata agaataataa
     3061 aggaaaatat caatgctaaa aaagacttta tcgatattct tgaatctgaa tgtttatttg
     3121 gagaagagta tttaatgttg tttgcatcat taagtgaatt agaattggca atattgggta
     3181 gtgtagtatt ttatgaagat attagaatga gagaaattaa tatattagaa tttatgttaa
     3241 atgagaaaac tgaaactact ttatggaaga tagagtacat taaatttatg aagaggcaaa
     3301 gtgaagataa gatatactcc atagaagagc atatgaataa aattaattaa caaaaaattg
     3361 tgaataaata gaatgttagt atataatata atctgtaaag catatcaaaa atacatatat
     3421 ttggaggaaa attatgaaag agtatatcat aaataatgtt gaagaaataa gagaagaatt
     3481 aattgattta tctaagaaaa tatgggaaaa tccagaatta gcctttgaag aaaagtatgc
     3541 aagtagcatt caaaaagaat acctaaagtc aaaaggattt aaaattgaag aagtagaaaa
     3601 tttacctact ggatttatag caagttttgg agagggtaaa cctgtgattg gcatacttgg
     3661 agaatatgat gctttaccag agctatcaca atgtgtaagt gcagaaagaa agccattggt
     3721 agaaggaaaa gctggacatg gatgtgggca caacctgttg ggtgtagcag gtgtaggagc
     3781 agttgtttct ataaaaaaat taatagaaga aaataaattt aatggaacaa taaaatattt
     3841 tggttgtcct gctgaagaag aaggtggagg aaagacagtt atgtgtataa atggctgttt
     3901 tgatgatgta gattgtgcat ttacatggca tccttttgat ataaatgcac cttggagagg
     3961 tggaagtctg gcaaacttat ctgtaaaatt taaatttaaa gggataacag cccatgcagc
     4021 tcaagctcct cataatggta gaagcgcact tgatgctgtt gaaattatga atgttggagc
     4081 taattatttg agagaacatg ttattgactc tataagaatg cactatgtta taactaatgg
     4141 tggtggaaga cctaatgtag ttcctggatt tgctgaaagt tggtacttta ttagaggtaa
     4201 aaaagcaaag gatgcagaac acgttcttga tagattaata aaagtagctc aaggagcagc
     4261 aatgatgaca gaaactgaaa tggagtataa ggttacagat ggaatatatg actatatacc
     4321 aaaccaatgt ttgactgatt tagtttatga caatatggtt tttgttggtt gccctaaaaa
     4381 tactcctgaa gaagaagaat ttgcaaaaaa gttgtgtgat actcttacta gagaagaaag
     4441 attaggtgta gcaacatcat tacaaaatga taaatctatt gtagaaagct atatacacaa
     4501 ggaaatagtt gacggtgaca aagataaagg actagctggt tctactgatg ttggagatgt
     4561 aagttatgta ataccagtgg ctcaatttgc aatggcagct tggcctgttg gaattgcaag
     4621 tcatacatgg cagtcatgtt catcagcagg ttcaaatata ggattttctg ctatgataaa
     4681 ttcagctaaa gtactggctt gtagtgctta tgatgtattt atggatacta aaattattga
     4741 tgaagcaaaa attgaatttg ataaatcatt agatggacaa aaatttaaac cattggtata
     4801 attttgctaa taaaatattt ctggggggaa tagctatgag agaaaaagtt gaaaaagtgc
     4861 ttgaggaaaa gataaaacca gtattacaaa gagatggtgg agatgtagag cttatagatg
     4921 taaatgaaaa tggtgtggtt ttagttagac tacaaggggc atgtagtggg tgtccaggag
     4981 tgactatgac aatcaaagct attattgaaa atgttttggt gagtgaagtt ccaggagtaa
     5041 ctcaagtatt aggtgtttaa tagttagcgt ttattaaatg tatgttatac ataagtattt
     5101 aataaagaag taaaaaaaat agtgaagcaa tatatatgaa aaagtatgag aaagataata
     5161 aaacattata gaaataatga aatatttcat atgtatatga agaaataaga aaaaatagtg
     5221 aagcaatata tatatgaaaa ag
//
LOCUS       DAMJYR010000209         5105 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.214, whole genome shotgun sequence.
ACCESSION   DAMJYR010000209 DAMJYR010000000
VERSION     DAMJYR010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5105)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5105)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5105
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.214"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            299..1636
                     /locus_tag="PC471_003658"
     CDS             299..1636
                     /locus_tag="PC471_003658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HDF2790740.1"
                     /translation="MGTYSLISTSINFILILINIFIAFFLYKLTSYFIDFRNHWIFKI
                     ILILGYSIISQMIIWLSDPINILFTLFGYFILLIICSKSKLIPIISVVMILYPIIIGL
                     NFLFINNPIYEELIHSKEYILILSIVQIISALIKLLFWFLIHIFLKNRLVNIKQYLSN
                     KIWIFIDMVCISSFLSILIAIILPPTNLIPMGSNKNIILGNLPYGTYLIILSAILANI
                     GVILLLQPLIENVKMKIEKQANHLKEKYYNLLENQQIQIRKMRHDMNNHFQMIESYLE
                     IEDIAGAKKYFNQLKIGIGTLSGKQFCKNQALNSILNLRHTSLEENGVDVHFNIDIDN
                     HLGIEPIDLCTIFSNSLDNAIEASLKIPDLSNRKVVLKARCEKEYFSYLLTNNKVNPI
                     NRKQELFISDKKDSNSHGYGIENIREIVNKYNGKLDISYTESEFSLFLYIRLF"
     gene            complement(1972..2541)
                     /locus_tag="PC471_003659"
     CDS             complement(1972..2541)
                     /locus_tag="PC471_003659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrpB family protein"
                     /protein_id="HDF2790741.1"
                     /translation="MKKKLEDMTLEELWNLFPIFLTTHKECWKNWFKEEQYRLTGILP
                     MQKIVRISHIGSTSVSTIYAKPIVDILIEIIPDYNMKDIKDLIESNGYICMSESSSRL
                     SLNKGYTVEGFEERVFHLHIRYVGDNDELFFRDYLIDHPDVAKEYEKLKLDLWKKYEH
                     NRDAYTDSKLDFVREFTEKAKVLYSNRYN"
     gene            2911..3516
                     /locus_tag="PC471_003660"
     CDS             2911..3516
                     /locus_tag="PC471_003660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urea carboxylase-associated family protein"
                     /protein_id="HDF2790742.1"
                     /translation="MTNWNLWRTLNMEYIINPCSGIKIKITRGQKIEIVDIEGGQVVD
                     FFAEMKDDSTEFISPGVTIDCNESLRLQVGNLIYSNKYRPMFKILYDDVREHDLLHPC
                     CRPEMYDFFYHNGENHPNCFDNINRTLGTSHQIIHPINLFMYTKVNTDGSIEVCEPLS
                     KANDKIILEALENMTLGIAACSVSESSCNSRKCTSIKILVD"
     gene            complement(4195..5097)
                     /locus_tag="PC471_003661"
     CDS             complement(4195..5097)
                     /locus_tag="PC471_003661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HDF2790743.1"
                     /translation="MLPQDYYGLIVILGVILGVMGAFYNWFTLYVQSLYKKIPKIGTF
                     GKLLIPFIMAGILGLVMPDVLGSGHNLVEELTSHKYIFGMVVLIFVIRFIFSAVSFGS
                     GAPGGIFFPLLVLGAFIGGIFGMVGVKVFGMNPDYVNNFVLLAMAGYFTAIVRAPLTG
                     IILIFEMTGSVSQMLSLSVISIVAYIVATLMKSKPIYESLLERLLEKNGEPVHKERGE
                     KILDQFSVMFGSPVANKAIQDVQWPENCLLVAIQRGGEEIIPRGKTILRPSDVIVTMT
                     DERDSGAVYDKMENLCKEHTAHIF"
BASE COUNT         1838 a          828 c          590 g         1849 t
ORIGIN      
        1 agcttagttt ttagagtttt ttcaaactta attttttaaa gctcagtttc tagaatttca
       61 atctttcaaa attcaatttt ttgaagctta gtttttagag tttttttcaa atttaatttt
      121 ttgaaactta atttttaaaa tttcaatctt ttaaaactca attttttgaa gcttaatttc
      181 tagaatttta tttttcaaaa tcaattcttt gaaatctaat ttccagaatt ttaattttta
      241 agatttaatt ttttaaaact tagtttccag aatcttaatt aatagaaagg tatattaaat
      301 gggcacttat tctttaatct ctacaagcat aaattttatt ctaatattaa tcaatatttt
      361 catagccttt tttctttata agttgacttc ctattttata gactttagga atcattggat
      421 tttcaaaatc atattgattt tgggctactc tattattagt caaatgataa tatggctttc
      481 cgaccccata aatattttgt ttacactatt cggatatttt atactactta taatatgtag
      541 caaaagcaaa ttaattccta taatatcagt tgtcatgata ctttatccta ttataattgg
      601 gcttaatttt ctttttataa acaaccctat atatgaggaa cttatacact caaaagagta
      661 tattttaata ctttcaatag tccagataat ttctgctcta attaaattat tattttggtt
      721 tttaatacat attttcctaa agaatagatt ggtcaatata aaacaatact tatcaaataa
      781 aatatggatt tttattgata tggtatgtat atcttcattt ttatcaatat taattgctat
      841 tattctgcca cctactaatt taatccctat gggtagtaat aagaatatta tcttaggcaa
      901 cttaccttat ggcacttatc taatcatctt gtcagcaatt ctggcaaata tcggtgtgat
      961 acttctttta cagccattga ttgaaaatgt aaagatgaag atagaaaaac aagcaaatca
     1021 cctaaaagaa aaatactata acttactaga aaatcaacaa attcaaatac gcaaaatgcg
     1081 acatgatatg aataatcact ttcaaatgat tgaatcgtat ttagaaattg aagatattgc
     1141 tggagctaaa aagtatttta atcagttaaa aataggtata ggtactctta gtggaaaaca
     1201 attttgcaaa aaccaagctc ttaactcaat tttaaatctt cgtcatacaa gtttagaaga
     1261 aaatggtgtt gatgtgcact ttaatataga tatagacaat cacttgggta tagaacctat
     1321 agatttatgt actatttttt caaattcttt agataacgct atagaagcat ctttgaaaat
     1381 tcctgatttg tcaaatagaa aagtggtatt aaaggcacgt tgtgagaagg agtattttag
     1441 ctatttattg acaaacaaca aggtaaatcc tatcaataga aaacaagaac tatttatttc
     1501 agataaaaaa gatagtaatt ctcacggata tggtattgag aatattagag aaattgttaa
     1561 taagtacaat ggaaaattgg atatttctta tactgagagt gaattttctt tatttcttta
     1621 tattagatta ttttagtcta aaattagatt atcttatata atacaaatta gattgtttga
     1681 tactatgtaa aattataata acttataatt aaatatttac acatatattc attaacttaa
     1741 aatttaatca aatgtgatat tttatttacc ttagtaaaaa tgtgtgtgtc tcaaaataaa
     1801 ctttttagtt gatgaggcac tcttttttac atgaaatcaa atatattttt atcctttcaa
     1861 caatgaaaaa tggatttatc tctccatttt gaaacaagcc ctttttctat taattacttt
     1921 cttaaaaaat ataatacgat atataagata aatatctaat tgtacctatt tctaattata
     1981 tctattagaa tacaacacct ttgccttttc agtaaactcc ctaacaaaat ctagtttact
     2041 atctgtataa gcatcacgat tatgctcata cttcttccaa aggtccaact taagtttttc
     2101 atactccttt gcaacatctg ggtggtcaat taagtaatct ctaaaaaaca actcatcatt
     2161 atcacctaca taccttatat gcaaatgaaa aactcgttct tcaaacccct ctactgtata
     2221 ccctttatta agtgaaagcc tactactgct ctcagacata caaatatatc cattcgactc
     2281 aatcaaatct ttaatatctt tcatattata atcaggaatt atctcaatca gtatgtcaac
     2341 aatgggcttg gcataaatcg tactcacaga agtacttcca atatgactta ttctaacaat
     2401 tttttgcatt ggcaatattc cagttagtct atattgttct tctttaaacc aatttttcca
     2461 acattctttg tgagtagtca aaaatatagg aaacaagttc cataactcct ctaaagtcat
     2521 atcttccaac ttctttttca ttagttacct ccttttaaat ttgtacttta tattttttct
     2581 ttttcaaatt tacttagtta atatttaact ttatttttta tcaaatcatt tttctaatta
     2641 tgtaaatttt tctaaattta gtttttaatg tattataaca gatttttacc cttcttacac
     2701 ttttgtatct atttaaatat tagaacattt tttcttactt ctaaatacag taagattgct
     2761 taaataccaa ataggagcaa aaaaactctg taatctaaaa aaaactacaa catctttatg
     2821 agaattaaaa aatgatagac tatgttatca acattaagaa acagatagaa aagaaacaga
     2881 gtatgtgcta aaatgggaac atagaacaga atgacaaatt ggaatttatg gaggacatta
     2941 aatatggagt atataatcaa tccttgtagt ggtataaaaa ttaaaataac tagagggcag
     3001 aaaattgaaa ttgtggatat tgaaggagga caggtcgttg atttttttgc agaaatgaaa
     3061 gatgattcaa ctgaatttat atctccaggt gttacaattg attgtaatga gtcattaaga
     3121 ttacaagttg ggaatttaat atattctaat aagtatagac ctatgtttaa aattttatat
     3181 gatgatgtaa gggaacatga cttgctacat ccatgttgta gacctgagat gtacgatttc
     3241 ttttatcaca atggagaaaa tcatccaaac tgttttgata atattaatcg cacacttgga
     3301 acttcccatc aaattattca ccccattaat ttgtttatgt acacaaaagt caatacagat
     3361 gggagtattg aagtatgtga acctttatca aaagccaatg acaaaattat tcttgaagca
     3421 ttggaaaaca tgactttagg gattgctgca tgcagtgttt cagaaagttc ttgtaatagc
     3481 agaaaatgta cttctattaa gattttggtt gattgacaaa tttcatttat cgaaaaaata
     3541 tcaaaattaa aaaatgacgt caaaggcgca aaagggtaac cacttgcgct ctcaattcat
     3601 tatggtttgt tacgtattta tgggtaaaaa tcccttgatt ctatgatgtt tttcagacac
     3661 aattacaaat gagtaaggat tttatactca tactctaaaa aatagcattt tattgcgtaa
     3721 cagaacgaaa aaacagtgtg tacctttttc ttgcaataca cactgtattc atagccactc
     3781 tagctgttaa ctgttaatta tctcataata cccttactgg tattcatcta atcttaaccc
     3841 cttttaatta ttttcattta taaataaatt ctattcttcc caaaatatcg taattaaaat
     3901 atattaatta aaatatccca ataagaatat ctgaaaaatc ttctacctat tttttcaaac
     3961 cttttattcc cattcctcat acaatagtgc aacttttagc acagtataaa tataccatta
     4021 gtccatataa tctcattaac aaatatatac aattattttc ttcaaactct tttatatcta
     4081 agatatcaca ttttcatttt cttatattaa ttccttaact ctctttcaaa ttaccaattt
     4141 tttaatcaaa tcttcattaa atattctatc attattaaac attcatcatt atccctaaaa
     4201 aatatgtgcc gtatgttctt tacacaaatt ttccatctta tcatacacag caccactatc
     4261 tctctcatct gtcatagtca caattacatc acttggtcta agaattgtct tacctctagg
     4321 aataatttct tctccacctc tttgaattgc taccaaaaga caattttcag gccactgtac
     4381 atcctgaatt gccttgttcg caacaggaga gccaaacata acagagaact ggtctagtat
     4441 cttttcgccc ctctctttat gtacaggttc tccattcttt tcaagtaacc tctcaagaag
     4501 actttcataa ataggtttag acttcataag tgtagccaca atatatgcca caattgaaat
     4561 tactgatagt gaaagcattt gactaacaga acctgtcatt tcaaaaatta atatgatacc
     4621 agtcaaaggg gcacgcacaa tagctgtaaa atatcctgcc atagccaaaa gcacaaagtt
     4681 atttacataa tcgggattca ttccaaaaac tttaacacct accataccaa atattccacc
     4741 tataaatgct cctaaaacaa gcaatggaaa aaatattccc cctggtgcac ctgacccaaa
     4801 actaacagcc gaaaatataa aacgtataac aaaaatcaaa acaaccattc caaaaatata
     4861 tttatgactt gtaagttctt caactaaatt gtgtccactc cccagaacat caggcataac
     4921 aagcccaaga ataccagcca ttataaatgg aatcaacagt tttccaaacg taccaatttt
     4981 aggaatcttc ttgtataaag actgcacata tagagtaaac cagttgtaaa atgcccccat
     5041 aactccaaga ataacaccta aaatcactat taatccataa taatcttgtg gcaacacatt
     5101 tccaa
//
LOCUS       DAMJYR010000210         5102 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.215, whole genome shotgun sequence.
ACCESSION   DAMJYR010000210 DAMJYR010000000
VERSION     DAMJYR010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5102)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5102)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5102
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.215"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            680..4812
                     /locus_tag="PC471_003662"
                     /pseudo
     CDS             680..4812
                     /locus_tag="PC471_003662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014466370.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF11 domain-containing protein"
BASE COUNT         1977 a          732 c          840 g         1553 t
ORIGIN      
        1 tatatataaa attcagaaaa aaattttagt ttacataata aaaataaagt caactaaaat
       61 tattgatttt agattaaagt accggattaa gcatttaagt agattttaag cttgatttga
      121 ttagaaaggt acaaactatc accgaaaaaa attaaaccca tttaaaaagc aatataaaag
      181 cttgttattt ttgagaaaaa tagaccctct aaactcaatt agattaaaaa taaaatacag
      241 aaaattatat ctctaaatat cttaaataat taagtttaaa ttttacgata ttttgtttga
      301 tgatttataa tataaaagca tactcttaat cttcaaaatt taaaaagagt aaaaaataac
      361 aaaaaaatca ctgacatttc actgacaaat tgtaattttg ttattataaa tacattgaaa
      421 atacttgatt attgaaaaat ggcataaaat aatacatttt atgccatttg ctattatttg
      481 atagttttta acttatgaca taagatattt tttaacatat acaataaaat ataaaaaaat
      541 tgatattgca aataaaaaaa aatacatatt agatgttata gaaaaaaagc gcacttatag
      601 gcttaaaatg tcacataaaa gagttttaaa gaataatata ttatgaacac aaattttgta
      661 atcacaaagg agtgataata tggcaacagt atcaggacaa atagtttttg atagaaatag
      721 aagtgctacc attgattctg gagactcagg aatagccaat ataccagttg tattacaaga
      781 tatagcaact ggagttagac ttgtggttct cacagatgca tcaggaaatt atgtttttac
      841 taatgttcca aatggtaact atagaattgt agaagctttt ggaactacag gaggagtacc
      901 aacaccagga aattttgcaa cagcgatagt aggtccagtt ccaattgcag aaatgccacc
      961 tattagttct gtgacaagtc ctcctattgg ttcaacaaac ttagatggta caacacctaa
     1021 cacactatta ataacagtag caggttcaga tataacaaat caaaatatat taaatgggcc
     1081 tgtgatttat acacctatag agaatatatt ggattcttgc gtatctgtat ctaatataaa
     1141 ccttattaca gatgcagata acggaacatt cggttttttt ccaccaggta caccaagtaa
     1201 tacaggacct gaaatagaac cttacccaaa tgtgacgcca gattttacgt atgttgtacc
     1261 agacccaact aagtttacac ctttagatgg agaatataca gtacaaaata ttatgacaaa
     1321 tgcaatgagt aatgttatag gagcttggtg gcgtattgct gaccatacta caggaaatga
     1381 aacaggaaga atgatggtgg taaatggttt taatcctgga gctgtatttt ttaaatcaac
     1441 agtacaagtt acacctaaca ctaattattt atttagcact tggattttaa acctttttaa
     1501 ggtaacagga tatccaccac cacagctagg tgtaagaata tttgaccaaa ataataatat
     1561 actatatcaa gcaacattag gagctcaaat tccagttaat acaaacgcac cagaatggaa
     1621 agaagtaggt actgtgatta attctcaaaa taataatcaa cttacagtag aattttttag
     1681 tgaaggagaa agcgccattg gaaatgacta tgctatagat gatgtttctt taagagaaat
     1741 agctgttcca acttttactc caataaaaac aagtaacaca gaaactgcta atgtaggtga
     1801 tataatcaca tatacagtta cattagataa tacttgtgat agccctttaa ctaatgtatt
     1861 ttttagggat ttaattccaa atggtttaga gtttatacct ggaactgtaa cggttgatgg
     1921 agtatctgta ccaggagtag accccaatac aggtttccca cttccagata ttggaggagg
     1981 aacaggggtt gaagtaactt ttgatgttgt ggttgaaagt attcctaacc caaatcctac
     2041 taataatata gctactatag attactccta tacacctgta gaaggcggta taccaaatga
     2101 tttttcagta gattctaatc cagttcctgt tgaagtaatc tcagcagata ttgaagttac
     2161 aaaattatct gaacctacta tagtgaatcc aggagaggaa ttaatataca ctataaaagt
     2221 agtaaataat ggaccattcc catctgaaaa tgtggtttta acagatgatg taccagcttc
     2281 tattgtaaat ccagagtatt cattagatgg aggagtaact tttcaaccat ggactggaag
     2341 tttaaatata gggacattag aagtaggaga gacaagagtt attataatta ggggtattgt
     2401 aaatccatca acagtaggaa ttataacaaa tacagctgta gtatcttcaa ctacagcaga
     2461 cccaaacctt aataataata catctacaat agagacagaa gtgaatttat tagcagatat
     2521 tttagtaatg aaaacagcag aacctaattc agctgttcca ggtactttat tgagatatac
     2581 tattcaagta gaaaacttag gaccagcaaa tgctgaaaat gtaatattaa atgatgatat
     2641 tccagcttct attataaatc cagaatattc attggatgga ggagcaagtt ttcaaccatg
     2701 gaatggaagt cttaatatag gaactttaaa ctctggaatt tcattaacag ttttaattca
     2761 aggaacagtt agtttaaatt cttcagaata tatagtaaat acagcaacag tttcttcaac
     2821 tacacctgac ccagatttaa gcaacaatat atctaccatt attacaccag ttaacccaca
     2881 agcaggtatt tctattataa aagtagctga cgaggatgta gctgttccag gagaagaatt
     2941 tgtatataca attgaaattt ttaatgaagg accatctaat gctacgaatg tagttttaac
     3001 agatgatata ccagatgtta ttttaaatcc agaatattca ttggatggag gagcaacttt
     3061 ccaaccatgg actggaagtc ttaatatagg tacagttgca ccagggcaat taataagaat
     3121 tatcataaag ggattagtaa gttcaactgc tacaggagat attactaata cagcagaagt
     3181 aagtgcagat gtaccagaac cagttacgga ttcatcaaca gtgacaactc ctatagttcc
     3241 atcagcagat atagaagtaa taaaaactag caatatggat acagcagttc caggagaaac
     3301 tttcagttat actataacag ttacaaattt aggaccatct gctgcacaaa gtatcgtttt
     3361 gacagatgat ataccagatg ttattttaaa tccagagtat tcattggatg gaggagtaac
     3421 tttccaacca tggaatggaa atcttagtat aggcactttg gatgctggag aaataagaag
     3481 tattataata agaggaacag tgagccagac agcagtaggt acaattataa atacagcaac
     3541 aataagttca actacacctg acccaaatcc agataataat acttcaacag atgaaacaga
     3601 tataagtcca ttagcagata tttctgtgat aaaaggcaac gaaccagttg ctataccagg
     3661 gggaagattt atttatggaa ttgaaattgc aaatgcagga ccatcttttg cagagaacgt
     3721 aactttaaca gataatatac cagcttctat tttaaatcca gagtactcaa tagataatgg
     3781 agtgactttc caaccatgga atggaagcct taatataggt actttagatg ctggagagat
     3841 aagaaatatc ataataaggg gaacagtaag tcaaacagca ataggcacaa ttataaatac
     3901 agcaacagta agttcaacta cacctgaccc aaatctaaat aacaatattt caacatcaga
     3961 agcagaggta agttcatcag cagatatttc tgtagtaaaa cgaagcaatc agactgttgt
     4021 tgtaccagga gatgtactag actatacaat tgaggtgaga aatgcaggac cgtctactgc
     4081 tcagaacgta actttaacag ataatatacc agcttctatt ttaagtccag aatattcaat
     4141 agataatgga gtgacttttc aaccatggaa tggaagtctt agtataggca ctttagatgc
     4201 tggagagata agaaatatta taataagggg aatagtaagt caaacagcaa taggtacaat
     4261 tataaataca gcaacagtaa gttcaactac acctgaccca aatccagata ataatacttc
     4321 aacatcagaa atagatataa gttcatcagc agatatttct gtaataaaat tagcaaataa
     4381 aacagaagca tgtgtaggag agcaaataga ttttgtaata gtagtatcaa atgcaggacc
     4441 agaaaatgct caaaatgtta ctttaataga caatgtttca gataagctta agaaagcagt
     4501 attttcactt gatagaggtg taagtttcca accatggact ggaagtctta atataggtac
     4561 actaccagct ggaactttaa gagtgattat attaagagga attataaaat caacatgtct
     4621 tgatagatta acaaatattg cagaaattac ttcaacaaca ccagattcaa accttaacaa
     4681 taatatatca agagtgcaaa acaatgttgt tgtaatgatt gctgttgtaa caactgtagt
     4741 aattgttgtt gcaatgattg tggttgtaaa gattgttgct gtaattgttg taaaaatgac
     4801 ttttggtgtt gattttataa aataattttg tataccttaa attaaactga aataaaaaat
     4861 aaattcacat atgagtaaaa tattttataa atattaataa attttctatc tttgatatgg
     4921 accacattcg taatactagt tattaaatga agtggtcctt tttaattcat taaaattatt
     4981 tattctaatt aaattttaaa tgtattaaat aattttaatt ttttataaaa aatattcata
     5041 tgttatttat attaaagttt agatatctaa aaatactttt agaatttatt atattatgtt
     5101 aa
//
LOCUS       DAMJYR010000211         5062 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.216, whole genome shotgun sequence.
ACCESSION   DAMJYR010000211 DAMJYR010000000
VERSION     DAMJYR010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5062)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5062)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5062
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.216"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            50..1933
                     /locus_tag="PC471_003663"
     CDS             50..1933
                     /locus_tag="PC471_003663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glycosidase"
                     /protein_id="HDF2790744.1"
                     /translation="MSNKNKENITLSNQIKGTIQNSLTKEAILHIPMSNYAYGYDRET
                     LHIRLRTKKNEAKRVILRIGDQYVWDKGGAGGGNLNASGVGWSGGTNIVMSKEVETEL
                     FDYWIAKCKPLNKRSRYGFIIEGQEEKLLFTEKRIIELGNENDEKELCEIGNFFGYPY
                     LNYIDIPKVPNWVKDTVWYQIFPDRFANGDPSINPEGVDKWGAIPTRDNFTGGDLQGV
                     IEHLDYLSDLGINGIYFCPITIGKTNHRYDTVDYMEVDPTLGDKETLKKLIEEAHKRN
                     IKIMLDAVFNHIGYYSKQWQDVVQNKENSRYKNWFYIKDMSKIDTPIEQIDEKNIPYE
                     TFGCEKYMPKLNTENSEVIKYLLDVGKYWIQEFDIDAWRLDVSNEVDHVFWRKFREEV
                     KKVKPDIYILGEIWHGSLPWLMGDQFDSVMNYLMSEAMKKFFCTDEINAEEFKYMIND
                     VMVSYPIQVSEVIFNLLGSHDTTRILTYANGNIDRFKLAYMFMFVQTGCPCIYYGDEI
                     GMEGELSSISEGQRKCMEWNEEKWNKDIFDFMKKIIRLRKENKELRSISNEWVLADKE
                     NGTIILKKESISIIINNSSKNIILTLPDYLANKKVKDLYEEEIVDLKEELMLEKYKFI
                     ILK"
     gene            2157..2837
                     /gene="pgmB"
                     /locus_tag="PC471_003664"
     CDS             2157..2837
                     /gene="pgmB"
                     /locus_tag="PC471_003664"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HDF2790745.1"
                     /translation="MIEAFIFDLDGVITDTAYYHYMAWRKLAHKVGIDIDTKFNESLK
                     GISRMESLDRILEFGNKKYSFSEEEKVRMAEEKNNYYVSLIDEITSNDILPGIESLLI
                     DVKSNNIKIGLSSASKNAINVLNHLGISDKFDFIADAGKCKNNKPHPEIFLMSAKGLN
                     VNPQNCIGIEDASAGIDAINSANMFSVGVGNYENLKKANLVVDSTNQLKFEYIQEKYN
                     EYIVRRII"
     gene            2834..>5062
                     /locus_tag="PC471_003665"
     CDS             2834..>5062
                     /locus_tag="PC471_003665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_097546124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HDF2790746.1"
                     /translation="MKCLFSNVDEWKIIQDKIEIKENRLAESIMSIGNGYMGMRGNYE
                     ENYSGDTHKGSYIAGVWFPDKTRVGWWKNGYPEYFGKIPNSVNYIGVRIFIDDVELDL
                     AKCKVEDFHRELDMKNGILNRRFIVNINDKKFEVRVTRFLSISVKELAVIKYSIKSLN
                     SNTKIKFVPYLDSNIKNEDSNYEEKFWNGISSSSLEYKGSILSRTKENAFNTPVFTVG
                     SMMYIKTNGDVEAVSHESDYNYCENSITMDVLKNEVASIEKYVSITSTRDYKESELLR
                     KCEDILNRELSKSYDDILNEHIKLWNKRWESADVKINGDKSSQQGIRYNLFQLFSTYY
                     GEDSRLNIGPKGFTGEKYGGATYWDTEAFCVPVYLGVADKEVTRNLLEYRYNQLEQAK
                     DNAKKLGLKGALYPMVTFDGVECHNEWEITFEEIHRNGSIVYAIYNYTNYTGDYKYIK
                     EKGIDVIIEIARFWASRVHLSTKKDLYMIHGVTGPNEYENNINNNWYTNYIAKWCLDY
                     AVENILRLEKECNECIDRNNVTREEVEFWKIISNKMYLLYDEELQIIIQNDNFLDKEF
                     VEVKDLPKENFPLNQNWSWDKILRSCFIKQADVLQGIYYFGNRFTKEEKKRNFDFYEK
                     YTVHESSLSSSIYSIIASEIDNLEKAYELYSRTARLDLDNYNNDTNDGLHITSMSGSW
                     LSIVHGFAGMRTWNETLSFDPKLPKEWKNYSFNINYRDNIINVDVCSDLIKIENLKGK
                     PIK"
BASE COUNT         2091 a          459 c          957 g         1555 t
ORIGIN      
        1 aacctatctt tagattatat aaattattat aaaggaggga aattttaaaa tgtctaataa
       61 aaataaagaa aatataactt taagtaatca aatcaaagga acaattcaaa atagtttaac
      121 aaaagaagct atattacata taccaatgag caattatgcc tatggttatg atagagaaac
      181 actacatata agacttagaa ctaaaaaaaa tgaagcgaaa agagtaattt tacgaatagg
      241 agaccaatat gtgtgggata aaggaggtgc aggaggagga aatttaaatg catctggagt
      301 tggttggtct ggtggaacaa atatagtaat gagtaaagaa gtagaaactg aattatttga
      361 ctattggata gctaagtgta agcctttaaa taaacgctca cgatatggct ttataataga
      421 aggtcaagag gaaaaacttt tatttacaga aaaaagaata atagagttgg gaaatgaaaa
      481 tgatgaaaag gaattatgtg aaataggtaa tttttttgga tatccatatt taaattatat
      541 agatatacct aaagtgccaa attgggtgaa agatactgtt tggtatcaga tatttccaga
      601 tagatttgca aatggtgacc cgtctataaa cccagaagga gtagataaat ggggagctat
      661 tccaaccaga gataatttta caggtggaga tttacaaggt gtgatagaac acttagatta
      721 tttgagtgat ttgggaataa acggaattta cttttgccct attacaattg gaaaaaccaa
      781 tcatagatat gatacggtag attatatgga ggtagaccct acattaggag ataaagagac
      841 tttaaaaaaa ctaatagaag aagcgcataa gagaaatata aaaataatgt tggatgctgt
      901 ttttaatcat ataggatatt attctaaaca gtggcaagat gtagttcaaa ataaggaaaa
      961 ttctcgatat aaaaactggt tttatataaa agatatgagt aaaattgata ctccaataga
     1021 acaaatagat gaaaaaaata taccttatga aacctttggg tgtgaaaaat atatgcctaa
     1081 acttaataca gaaaattcag aagtaataaa atatttattg gatgtaggca agtactggat
     1141 acaggaattt gatatagatg cgtggagatt agatgtttca aatgaagtag accatgtttt
     1201 ttggagaaag tttagagaag aagtcaaaaa agtaaaacca gacatttata tattgggaga
     1261 aatttggcat ggtagtttac catggttaat gggtgaccaa tttgattcag ttatgaatta
     1321 cttgatgtca gaagccatga aaaaattctt ttgtacagat gagataaacg ccgaagaatt
     1381 taagtatatg ataaatgatg taatggtaag ttatccaata caagttagtg aagttatttt
     1441 taatttactt ggaagtcacg atactacaag aatattgact tatgctaatg gaaatattga
     1501 tagatttaaa cttgcatata tgtttatgtt tgtacaaaca ggatgtcctt gtatatacta
     1561 tggtgatgaa atcggaatgg aaggagaatt aagttcaata tctgaaggtc aaagaaaatg
     1621 tatggaatgg aatgaagaaa aatggaataa ggatatattt gattttatga agaagattat
     1681 tagacttcgt aaagaaaata aagaacttag gtctatatca aatgaatggg tgttagcaga
     1741 taaggaaaat ggaacgatta tattgaaaaa agaatctatt tctataataa taaataattc
     1801 atctaaaaat ataattttga cgttaccaga ttatttagca aataaaaaag taaaagattt
     1861 atatgaagaa gaaatagttg atttaaaaga agaactaatg ttagagaaat acaagttcat
     1921 tattttaaaa taatacaaaa aagtatttat tttagatttc atgttaaatt attatacagt
     1981 ttaaacaagt ctttataaac gataaaaaat atggtctaca agtgagtgaa ttgaaagtag
     2041 attttaaatt aaagactgag ttagagtaga taattttata aataatatct taaatacaag
     2101 atagaaaata aattgattat tagtctaaaa atttcttgta gaaagaggaa ataagaatga
     2161 ttgaagcatt tatatttgat ttagatggtg taattacaga tactgcatat tatcactata
     2221 tggcttggag aaaactagct cataaagttg ggatagatat agacacaaaa tttaatgaat
     2281 ctttaaaagg cataagtaga atggagtcat tggacaggat attagaattt ggaaataaga
     2341 aatattcatt ttcagaagaa gaaaaagtga gaatggctga agaaaaaaat aattactatg
     2401 tatcattgat agatgagata acttcaaatg atattttgcc aggcatagaa agcttgttaa
     2461 ttgatgtaaa atctaataat ataaagatag gtctatcttc tgcaagtaag aatgcaataa
     2521 atgtattaaa tcatttgggt ataagtgaca aatttgattt tatagctgat gcaggaaaat
     2581 gtaaaaacaa taaaccacat ccagagatat ttcttatgtc agcgaaaggg cttaatgtaa
     2641 atccacaaaa ttgcataggg atagaggatg caagtgcagg tatagacgct ataaactcag
     2701 ctaatatgtt ttctgttggt gttggaaatt atgaaaactt aaaaaaagca aatttagttg
     2761 tagattcaac aaatcaatta aagtttgaat atatacaaga aaagtataat gagtatatag
     2821 ttaggagaat tatatgaaat gtttatttag taatgtagat gagtggaaga ttatacaaga
     2881 taagattgaa atcaaagaaa atagattagc agagtccata atgagtatag gcaatggata
     2941 tatgggtatg agaggaaact atgaggaaaa ttatagtgga gatacacaca aggggtctta
     3001 tatagctgga gtatggtttc cagataaaac tcgtgttggt tggtggaaaa atgggtatcc
     3061 agaatacttt ggtaaaattc ctaattcagt caactatata ggtgtaagaa ttttcataga
     3121 tgatgtagaa ttagatttag caaaatgtaa agtagaagat tttcatagag aattagatat
     3181 gaaaaatggt attttaaatc gtaggtttat tgtaaatata aatgataaaa aatttgaagt
     3241 tagggttact aggtttttaa gtatatctgt taaggaactt gcagtaatta aatacagtat
     3301 aaaatcatta aattctaata ctaaaataaa atttgtacca tatttagatt caaatataaa
     3361 aaatgaagat tcaaactatg aggaaaagtt ttggaatgga atttcttcaa gttctttaga
     3421 atataaaggg agtattcttt ctcgaacaaa ggagaatgca tttaatacac cagtattcac
     3481 tgtgggttca atgatgtata ttaagacaaa tggtgatgta gaagctgtat ctcatgagag
     3541 tgattataat tactgtgaaa attcgataac aatggatgtt ttaaaaaatg aggttgcaag
     3601 tatagaaaaa tatgtatcta taacctcaac tagagattat aaggaaagtg agctattaag
     3661 aaaatgtgaa gacattttaa atagagaact atctaaaagt tatgatgata tattaaatga
     3721 acatataaag ttatggaata aaagatggga aagtgcagat gtaaaaataa atggagataa
     3781 aagtagccaa caaggtataa gatataattt attccaacta ttttcaacat attatggaga
     3841 agattcgcga ttaaatatag gtccaaaagg atttactggt gaaaagtatg gaggagctac
     3901 ttattgggat acagaagcgt tttgtgttcc agtatatcta ggtgtagcag ataaggaggt
     3961 aactagaaat cttttagaat ataggtacaa tcagttagaa caggcaaagg ataatgcaaa
     4021 aaaactaggt cttaaaggtg ctttatatcc aatggtaaca tttgatggag tagaatgtca
     4081 taatgaatgg gagataacat ttgaggaaat ccaccgaaat ggaagtatag tatatgcaat
     4141 atataactat acaaattata caggtgacta taaatatata aaagaaaaag gaatagatgt
     4201 aataatagaa atagctagat tttgggcaag tagagttcat ttgtctacaa aaaaagattt
     4261 gtatatgata catggagtaa caggtccaaa cgagtatgaa aacaatatca ataataattg
     4321 gtatactaat tatatagcta aatggtgttt agattatgct gtagaaaata tcttacgatt
     4381 agagaaagag tgtaatgaat gtattgacag aaataatgtg actagggaag aagttgagtt
     4441 ttggaaaata atatcaaata aaatgtatct gctgtatgat gaggaactac aaataataat
     4501 tcaaaatgat aattttttag ataaagaatt tgtagaagta aaggatttac caaaagaaaa
     4561 ttttcctctt aatcaaaatt ggtcttggga taagattctt aggtcgtgtt ttataaaaca
     4621 agcagatgta ttacagggaa tttactattt tggaaataga tttactaaag aagaaaaaaa
     4681 gagaaatttt gacttctatg aaaaatatac tgtacatgaa tcttctttat cttctagtat
     4741 ttattctatt attgcctcag aaatagataa cctagaaaag gcttatgaat tatattcaag
     4801 aacagctagg cttgatttag ataattacaa taatgataca aatgatggac tgcacataac
     4861 ttcaatgtct ggttcatggc tttccattgt acatggattt gcaggcatga gaacatggaa
     4921 tgaaactcta tcttttgacc ctaagttgcc aaaagagtgg aaaaattatt ctttcaatat
     4981 aaattataga gataatatta ttaatgtaga tgtatgtagt gatttaatta aaatagaaaa
     5041 tttaaaaggt aaaccaataa aa
//
LOCUS       DAMJYR010000212         4967 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.217, whole genome shotgun sequence.
ACCESSION   DAMJYR010000212 DAMJYR010000000
VERSION     DAMJYR010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4967)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4967)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4967
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.217"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     misc_binding    273..504
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            612..2093
                     /locus_tag="PC471_003666"
     CDS             612..2093
                     /locus_tag="PC471_003666"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HDF2790747.1"
                     /translation="MYYSSTRGTEEKVTASQAIIKGISNDGGLYVPSEFPNVKNELIN
                     LVNLTYSQIAFFVLSKFLCDFTEDEIKNCIENAYDEKFDCSSIAPLNKVNDTYFLELY
                     HGPTLAFKDMALTIMPHLLKTSIKKDNLEKDVVILTATSGDTGKAALEGFKDIDKIKI
                     IVFFPEDGVSPVQKLQMKTQTGKNTYVVGIKGNFDDAQSGVKKIFSDKEFNQKLLDNN
                     YILSSANSINIGRLLPQVAYYFYSYMKLVSNSDINLNDKINFVVPTGNFGNILAGYYA
                     KQMGLPINKLICASNDNNVLYDFFKTGVYDKNRTLKLTTSPSMDILISSNLERLLFEI
                     SNRNTDIVNKLLSDLSDKGVYKISDDMEKNLASFYGEYSNEEEVKSTINNVFKNYNYL
                     IDTHTAVAYNCYEKYKKETSDNTKTVIVSTASPFKFSEDVLKSIDCDFKDLDDFAIID
                     RLSSISKIDIPKPIKNLKNAEILHKDIYEKDELKLAIKKFLKV"
     gene            2103..2996
                     /locus_tag="PC471_003667"
     CDS             2103..2996
                     /locus_tag="PC471_003667"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HDF2790748.1"
                     /translation="MLEIIVPATSANIGPGFDCLGIALNIYNKFYVEEIESGLEIEGC
                     EDAYKNENNLVYTSMKYFFDRVKPEKIPAGIKIKIQSEVPICRGLGSSASCIVAGVIA
                     ANALSGANLDKNQLLNIASEIEGHPDNVAPAILGNMIVSVTDNENIHYDIIKIPEELK
                     FCAMIPNFKLSTEKARGVLPKEIPYSDGVFNVSRVALLISALLNKNFDLLKVACQDKL
                     HQDYRGTLIENYNDIVEKSEQLNSIGVFLSGAGPTIMSLIKENDDSFVDNMKNYLQKL
                     KSDWEIKELCCDSNGAVLNII"
     gene            3120..4181
                     /locus_tag="PC471_003668"
     CDS             3120..4181
                     /locus_tag="PC471_003668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015617764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HDF2790749.1"
                     /translation="MIIKELIIRDLVAKVPIIQGGMGVGISRSSLAGAVAKLGGVGVI
                     SGVQIGYDEEDFETNTINANLRAIKKHISKAKEISNGGIIGINFMVAMKEYETYVKEA
                     VKAGVDLIISGAGLPNKLPSLVKGSNVKIAPIVSTAKAANVILKMWDRKEKTTADLIV
                     VEGPKAGGHLGYSNEELDNIDSIDYDKEFVEILKVANTYGEKFGRNIPVVAAGGITSS
                     SDVKKYIDMGASGVQVGTRFVATYECDAHENFKMAYINASEEDVHIVKSPVGLPGRAI
                     RNKFIEEVKINRPEIKKCYNCLIPCNPKETPYCISQALINAVKGDVENSLLFCGNDAY
                     KIDKLSTVEDVINELISEL"
     gene            complement(4336..4848)
                     /locus_tag="PC471_003669"
     CDS             complement(4336..4848)
                     /locus_tag="PC471_003669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HDF2790750.1"
                     /translation="MSDFKEILAEEIKNNPFGLIGKDWTLITAENEGKVNTMTASWGG
                     LGVMWGKDVAFVVIRPQRYTKEFIDNTDKFSLTFFDEDFRKELSYCGKVSGREEDKIS
                     QIGFNIEHLNDTPYFKESKMAIICKKMYSQKLEPQCFIAEGIDGRWYPQKDYHTLYIA
                     EITNVLVKED"
BASE COUNT         1851 a          608 c          768 g         1740 t
ORIGIN      
        1 ttttgtaaac tttttttgta atatagtgta gtataaaatt gtaatattac agatatttaa
       61 tagttatctt ttaattactt tcattaaaaa cagcttcatc actatgaaaa tctaggtttt
      121 taaactttta aaaaagttaa aaaaatattt ttctaatact caatttgatt acaatttttt
      181 tacaatttaa aaaatatttt ttccaaattt ttatattaat acttgacttt gtctgtttta
      241 taaactagaa taattaataa tattaagaat attgaaaggc attgattagg aagagtaaat
      301 ctgaatcgat taccagagaa ggaaactact agctgaaatg tttccctaat tagaaggatt
      361 gaaaactacc taggagcttc tatccgaagt tatcttaatg attttagtag gaatagacgt
      421 ttttattact tgcgttaaag tatcaagtgg attttttaaa atcaatttgg gtggaaccgc
      481 gggaaacaga ttctcgtccc ttttttaggg atgagggtct ttttttatta cctaaattca
      541 gtttttaaaa tattctgtat ctaatttaat tagctaatta aaaatttaaa atatatggag
      601 gattataaaa aatgtattat tcaagtacga gaggcaccga agaaaaagta actgcatcac
      661 aagcaataat aaaaggaata tcaaatgatg gtggtcttta tgttccaagt gaatttccca
      721 atgtaaaaaa tgaacttata aatttagtaa atttaacgta ttctcagata gcattttttg
      781 tacttagtaa atttttatgt gattttactg aagatgaaat taaaaattgt atagaaaatg
      841 catatgatga aaagtttgat tgtagtagta tagctccatt aaataaagta aatgatactt
      901 atttcttaga actatatcac ggtccaactt tagcatttaa agatatggca cttactataa
      961 tgcctcatct tcttaaaaca tctataaaga aagataattt agagaaagat gttgtgattc
     1021 ttactgctac atctggagat acaggtaaag ctgcacttga gggatttaaa gatatagata
     1081 aaataaagat aattgtattc ttcccagaag atggtgtaag cccagtacaa aaacttcaaa
     1141 tgaaaactca aactggaaaa aatacatatg tagttggtat aaaaggaaat tttgatgatg
     1201 cacaatcagg tgtaaagaag attttttctg ataaagaatt taatcaaaaa ctattggata
     1261 ataattatat attatcttct gcaaattcaa ttaatatagg aagacttctt ccacaagttg
     1321 catactattt ttattcatat atgaaattag ttagcaatag tgatataaat ttaaatgata
     1381 aaataaattt tgttgtccca actggtaatt ttggtaatat tctagctgga tattatgcta
     1441 aacaaatggg tcttccaata aacaaattaa tatgtgcatc aaatgataat aatgttcttt
     1501 atgatttctt taaaactggt gtatatgata aaaatagaac attgaaatta actacttctc
     1561 cttctatgga tatattaatt tcaagtaatt tagaacgatt actgtttgaa atatcaaata
     1621 gaaatactga tattgtaaat aagcttttat cagaccttag tgataaagga gtctacaaaa
     1681 taagtgatga tatggaaaaa aatttagcct cattctatgg agaatattca aatgaagaag
     1741 aagttaaaag cacgataaac aatgtgttta aaaattataa ttatttgata gatactcata
     1801 ctgctgttgc atacaattgt tatgaaaaat ataaaaaaga aacttctgat aacacaaaaa
     1861 ctgttattgt aagtactgca agtccattca agttttctga agatgttcta aagtctatag
     1921 attgtgattt taaagacctt gatgactttg ctataattga tagattatca agtatttcta
     1981 aaatagatat tccaaaacct attaaaaatc ttaaaaatgc agaaatacta cataaagata
     2041 tttatgaaaa agatgaatta aaactagcta taaaaaaatt cttaaaggta taggtgatta
     2101 ttatgttaga gattatagtt cctgcaacaa gtgctaatat aggtccagga tttgattgtc
     2161 ttggaattgc gttaaatata tacaataaat tttatgttga agaaattgaa agtggtcttg
     2221 aaatagaagg ttgtgaagat gcttacaaaa acgaaaacaa cttagtttat acatctatga
     2281 agtacttttt tgatagagtt aaaccagaaa aaatacctgc tggtatcaaa ataaaaatcc
     2341 aaagtgaagt acctatatgt agaggtcttg gcagtagtgc ctcttgtata gtagctggag
     2401 ttatagctgc aaatgctcta tcaggtgcta atttagataa aaatcaatta ctaaatatag
     2461 cttcagaaat agaaggtcac ccagataatg ttgctcctgc tattttagga aatatgattg
     2521 tatctgttac tgataatgag aatatacatt atgatattat aaaaattcct gaagaactta
     2581 aattttgtgc aatgatacct aattttaagt tatcaacaga aaaagctaga ggtgttttac
     2641 caaaggaaat accatattct gatggggtat tcaatgttag tagagttgct ttactaatat
     2701 ctgccttact aaacaaaaac tttgacctac ttaaagtagc ttgtcaagac aaattgcatc
     2761 aagattatag aggaactcta attgagaact acaatgatat tgttgaaaaa tctgaacaat
     2821 taaatagtat tggagtattt ttaagtggag ctggacctac tataatgtct ttaataaaag
     2881 aaaatgatga tagttttgtg gataatatga aaaattacct acaaaaacta aaatctgatt
     2941 gggaaataaa agagttatgt tgcgattcta atggagctgt tttaaatata atataattat
     3001 cgtcttctat acaaaatata actactcttt tattataatt tgtagtataa taattaggac
     3061 taggtaataa tattaagtat tataatctag ttctttaaac atgtttacat tgaggaggga
     3121 ttatcataaa agagttaata attagagatt tagttgcaaa agttcctata attcaaggtg
     3181 gaatgggcgt tggtatatct aggtcatcac tagctggtgc agttgctaag cttggtggtg
     3241 ttggtgtaat ttctggagtt caaattggat atgatgagga agattttgaa actaatacta
     3301 taaatgcaaa tttaagagct ataaaaaaac atatttctaa ggccaaagaa atctcaaatg
     3361 gtggtattat tggtattaat tttatggtag ctatgaaaga atatgaaaca tacgtaaagg
     3421 aagctgttaa agctggtgta gaccttataa tatctggagc tggattacct aataagctac
     3481 catcattagt aaagggaagt aatgtaaaaa tagcacccat agtttcaact gcaaaagctg
     3541 ctaatgtaat tttaaaaatg tgggatagaa aagagaaaac tactgctgac ttaatagttg
     3601 ttgaaggtcc aaaagctggt ggtcaccttg gatactctaa cgaggaactt gataacatag
     3661 attctataga ctatgataag gaatttgttg aaattttaaa ggttgcaaat acttatggag
     3721 aaaaatttgg tagaaacata ccagtagttg ctgctggtgg tataacttct agtagtgatg
     3781 ttaaaaaata tatagatatg ggtgcaagtg gtgtacaagt tggtacaaga tttgttgcta
     3841 cttacgaatg tgatgcccat gagaacttta aaatggcata cataaatgct agtgaagaag
     3901 atgttcatat agttaaaagt cctgtaggtc ttcctggacg cgccataaga aacaaattta
     3961 tagaagaagt taaaataaat cgtccagaaa taaagaagtg ctataattgt ttgattcctt
     4021 gtaatccaaa agaaactcct tactgtattt ctcaagctct aatcaatgca gttaaaggag
     4081 atgttgaaaa ttcactttta ttttgtggaa atgatgccta taaaattgat aaattaagta
     4141 ctgttgaaga tgttatcaat gaattgattt ctgaattata atttaaagaa aactaaatta
     4201 aaattttatc aagctaagga gtttaaatac gttatttatt aatctttata aacatattta
     4261 tgtccttagc ttttttgtgt caatttcctt ttatggaaat tttaatttaa ttagagatag
     4321 tattaacctt tataactaat cttcttttac aagtacattg gttatttcag ctatatataa
     4381 agtatgataa tctttttgtg gataccatct tccatctata ccttcagcaa taaaacattg
     4441 aggttcaagt ttctgactat acattttttt gcatataata gccattttag attctttaaa
     4501 ataaggtgta tcatttaagt gttctatatt aaatccaatc tgtgatatct tatcctcttc
     4561 tctaccagat acctttccac aatatgaaag ctctttacga aaatcttcat caaaaaatgt
     4621 aagagaaaat ttatctgtat tatctataaa ttccttagta taacgctgtg gtcttattac
     4681 tacaaaagca acatcttttc cccacataac tccaagccct ccccagctag cagtcattgt
     4741 attcacttta ccttcatttt ctgctgttat aagtgtccaa tctttaccta tcaatccaaa
     4801 tggattattt ttaatctctt ctgccaaaat ctctttaaaa tcactcatat ctctaataac
     4861 ctctcattct cttatattta tattcctttt attttcgtga taaattataa atgaagtcta
     4921 gttttgaaaa taaaatactt ttttaaatat attattgcaa ataagta
//
LOCUS       DAMJYR010000213         4752 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.218, whole genome shotgun sequence.
ACCESSION   DAMJYR010000213 DAMJYR010000000
VERSION     DAMJYR010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4752)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4752)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4752
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.218"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            54..1361
                     /locus_tag="PC471_003670"
     CDS             54..1361
                     /locus_tag="PC471_003670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2790751.1"
                     /translation="MKEIADAITSEYLSYGINEDISIYEGRFCIYLDKKYRCNGKIYY
                     KMTPPISISFKADIGCVEEIDNEDDNLALDYDNAILEVHGYKIISITINTLSEFSVEG
                     YINDDCIKSKNSYVEYVDFNIINLDKIPGKLIKYNDKVYAGRIEFDINDYVVTIDKRY
                     DYRKELKSELKSKSGAIITHIGRIRRKDGRIFRTNNTINLLDRISTALSFMCGRYVGF
                     CLAKGYRSGNEVYRIWNENQISPFRYVPTWSDTLSNYHNMEKYISLMCKKLEDFYYGS
                     AIKSVVDWYIESLGSATMENNIISVQIALETLSYVILVEQNKILTDEAFDCNLASKNI
                     RLLLDTCKIPYGKHELNIFDNIIKNKFDDGVDLVIYLRNSIVHPSRKTHRAVLEVEDI
                     WNIISIGTRYIELVLLFILGYRGEYSNRLVERCYGEVEVVPWN"
     misc_binding    1447..1709
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1835..2638
                     /locus_tag="PC471_003671"
     CDS             1835..2638
                     /locus_tag="PC471_003671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HDF2790752.1"
                     /translation="MKNILKKVGIFTIMLGLLGGVVGCSKPDNEKDKDASKESKKEVV
                     VGFDNTFVPMGFLDEKGDTVGFDVDLAKETFKRLGMEVKFQPIDWSMKETELNDSKTV
                     DVLWNGYSITDERKKIVSYTEPYLQNKQIIVTLSDSKINSKADLKDKEVGTQQGSTAL
                     DAVEKDKDFMNSLKGGAPVLYDTYDKALRDLEIGRTSAVVGDEVLIRYYMGQKGEDKY
                     KVLKDDFGLEDYVVATSKENPELCEKINETLKEMKKDGTFDKIYDKWFK"
     gene            2676..3287
                     /locus_tag="PC471_003672"
     CDS             2676..3287
                     /locus_tag="PC471_003672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HDF2790753.1"
                     /translation="MLQGLEIVIAMFCITLIVSIPLGIGVAFLRLSKNKLVSGITQCY
                     ILIMRGTPLLLQMIVIFYGLPLLGIVFDRFTAGVVAFFLNYAAYFAEIFRGGIQSIDR
                     GQYEASKVLGFDKFTMYKRVIFPQVFKRILAPISNEVITLVKDTSLVYILGLNDILRI
                     SQIAMNREASLLPLFEAGAIYLIFVAILTKGFELLEKKYSYYR"
     gene            3319..3990
                     /locus_tag="PC471_003673"
     CDS             3319..3990
                     /locus_tag="PC471_003673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HDF2790754.1"
                     /translation="MLKIKNLNKSFKKNRVLKDISFELEEGQIGVLLGKSGAGKTTIL
                     RCINGLEEFDSGEIIIDNEVIKNKRDMAKIRGKIGMVFQNFNLFPHMTVLENIIESPV
                     NVFKVPRKEAEERARELLRLVDLEDKLNSYPFELSGGQQQRVAIARSCALMPKVLCFD
                     EPTSALDIDTIQRVVNIMNRLKDKGMTILIITHDVVFSNNVADKIISIKDGIVENVQI
                     KEKIV"
     gene            4289..4651
                     /locus_tag="PC471_003674"
     CDS             4289..4651
                     /locus_tag="PC471_003674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HDF2790755.1"
                     /translation="MIKLYGYTKCSTVKKAKNWLKENNLEFEDIDMVQNPPSKEELKS
                     IYKTSGYDIKKFFNTSGMKYRELGLKDIVKTESDDKLLEILVSDGMLIKRPLLLDGKN
                     VLLGFKEDVWKSTLLKED"
BASE COUNT         1904 a          436 c          862 g         1550 t
ORIGIN      
        1 attaaattat ataatatatt gagttttaca taaaagcaaa ggtgggaata aaaatgaaag
       61 aaattgctga tgctataaca tcagagtatt taagctatgg tatcaatgaa gatattagta
      121 tctatgaagg tagattttgc atatatttag ataaaaaata tagatgtaat gggaaaattt
      181 attataaaat gacacctcca atatctataa gttttaaagc agacataggt tgtgttgaag
      241 agatagacaa tgaagatgac aatttagctt tagattatga taatgctatt cttgaagttc
      301 atggatataa aattatttct ataacaataa atactttaag tgaatttagt gtagaaggtt
      361 atatcaatga tgactgtata aaatctaaga actcctatgt tgaatatgta gattttaata
      421 taataaactt agataaaata cctggaaaac tcattaaata caatgataag gtatacgcag
      481 gtagaattga atttgatata aatgattatg ttgtaactat agacaagcga tatgattata
      541 gaaaagaatt aaaatcagaa ttaaagtcta aatctggagc aataataact catataggca
      601 ggattagaag aaaagatggt agaattttta gaacaaataa tacaataaat cttttagata
      661 ggatatcaac agctttgagt tttatgtgtg gaagatatgt agggttttgt ttggccaaag
      721 ggtatagaag tggtaatgaa gtttatagaa tatggaatga aaatcaaata tctcctttta
      781 gatatgtacc aacatggtca gatacacttt caaattatca taatatggaa aaatatataa
      841 gccttatgtg taaaaagcta gaagattttt attatggttc tgctataaag agtgttgttg
      901 attggtacat agaatctctt ggaagtgcta ccatggaaaa caatataata tctgtacaaa
      961 ttgcacttga aactttgtca tatgtaattc tagtggaaca aaataagata cttacagatg
     1021 aagcatttga ctgtaatttg gcatctaaaa atataagact cttacttgat acatgtaaga
     1081 taccatatgg taaacatgaa ttaaatatat ttgataatat aataaaaaat aaatttgatg
     1141 atggagtgga tttagttatt tatttaagaa atagcatagt tcatccaagt agaaagactc
     1201 atagagctgt gttagaggtt gaagatattt ggaatataat ttcaatagga acgagatata
     1261 ttgaacttgt tttactcttt attttaggtt atagaggaga atattcaaac agattagtag
     1321 aaagatgtta tggtgaagtg gaagttgtac cttggaacta gatttttata tttattttaa
     1381 aatatatatt gacatatatt gccttatgct ataaaatcaa tatcataata ataattcaga
     1441 ataacattaa atgctaagaa aagaaatagt aaaagtagtt aatcttaaac agagagtttc
     1501 cgattggtga gaggagcaat agattttact ttgaacacat ctttgagcag gactctgaaa
     1561 gagtagaaat actttattag gagatttccg gaatccttgc acccgttata gtgctagagt
     1621 ataataattt tattgtactt aaagaggtta gtgccgtgag gcattaatga acagaggtgg
     1681 aaccacgaga gttaactccc gtcctctagc agaagctaga ggcgggagtt ttttaatata
     1741 attttttaag cgcgcagaga attatattgc aatagataca ctttttaaaa aataatcaaa
     1801 atattttaaa aattaaaaga cgagggagaa tattatgaaa aatatattaa aaaaagtagg
     1861 aatatttaca attatgttag gtttattagg tggagttgta ggttgttcaa aaccagataa
     1921 tgaaaaagat aaggatgctt caaaggaatc taaaaaagaa gtagttgtag ggtttgataa
     1981 tacgtttgta ccaatgggat ttttagatga aaagggagat acagtaggct ttgacgtaga
     2041 cttagctaaa gaaacattta aaagacttgg aatggaagtt aagtttcagc ctatagattg
     2101 gtcaatgaag gaaactgaat taaatgattc aaaaactgta gatgtattat ggaatggata
     2161 ttctataaca gacgaaagaa agaaaatagt atcatataca gaaccatatt tacaaaataa
     2221 acagataata gttactctaa gtgactcaaa aataaattca aaagctgatt taaaagataa
     2281 agaagttgga acacaacaag gttcaacagc acttgatgca gtggaaaagg ataaagattt
     2341 tatgaatagc ttaaaaggtg gagcaccagt tctttatgat acttatgata aagcacttag
     2401 agatttagag ataggtagaa ctagtgcagt tgttggtgat gaagttttaa ttagatacta
     2461 tatgggacaa aagggtgaag acaaatacaa ggtattaaaa gacgattttg gattagaaga
     2521 ttatgttgta gcaacttcta aagaaaatcc agaactttgt gagaaaataa atgaaacatt
     2581 aaaagaaatg aaaaaagatg gaacttttga caagatttat gataagtggt ttaaatagaa
     2641 gataagttag ctgttaagaa gggggcacta aaggcatgtt acaaggttta gagatagtta
     2701 tagcaatgtt ttgtataaca ttgatagtat caattccatt ggggattgga gttgcatttt
     2761 taagattatc aaagaataaa ttagtaagtg gtattacaca atgttatata ttgataatga
     2821 gaggtacacc tttacttttg caaatgatag tcatatttta cggattacct cttttaggaa
     2881 ttgtatttga cagatttaca gctggagtag tagctttctt cttaaattat gcagcatatt
     2941 ttgcagaaat atttagaggt ggaatacagt caatcgatag aggacaatat gaagcttcaa
     3001 aagtactagg atttgataag tttactatgt ataagagagt aatctttcca caagttttta
     3061 aaagaatact agcacctatt tcaaatgaag taatcacatt agtaaaagat acatctttag
     3121 tatatatatt aggattaaat gatattttaa gaatatctca aatagcaatg aacagggaag
     3181 caagtctgtt accattattt gaggcaggag cgatatatct tatatttgta gctatactta
     3241 ctaagggatt tgaattactt gaaaaaaaat attcttatta tagataaagt tacaatatta
     3301 taggaggagg ttttttatat gttgaaaatc aaaaacttaa ataaatcatt taaaaaaaat
     3361 agagtactta aagatatttc ttttgagtta gaagaaggtc agattggagt tttacttggt
     3421 aagtctggtg caggaaagac cacaatatta aggtgtataa atggtcttga agaatttgat
     3481 agtggagaga ttataataga taatgaggta ataaaaaata agagagatat ggcaaaaata
     3541 cgaggaaaaa taggtatggt ttttcaaaat ttcaacttat ttcctcatat gacagtcctt
     3601 gaaaatataa ttgaatcccc agttaatgtt tttaaagttc ctagaaaaga ggctgaagag
     3661 agggctagag aacttttaag attagttgat ttagaagata aattaaattc ttatccattt
     3721 gaattgtcag gaggacagca acagagagtt gctatagcta ggtcatgtgc attgatgcca
     3781 aaagtccttt gttttgatga acctacttct gcacttgata ttgatactat tcaaagagta
     3841 gtcaatataa tgaacagact taaagacaaa ggtatgacaa tattaataat tacacatgat
     3901 gttgtcttct caaataatgt tgcagataaa attataagta taaaagatgg tatagtagaa
     3961 aatgttcaaa taaaagaaaa aattgtttag agttttataa atataaacta gggtagaatt
     4021 ttataatata aataaagggc aagtaaaatc aaatatacta tgcccttttg ttatctcaaa
     4081 atttaatctt atattttatg tttaagttac attataattt ataataaaaa taaaaggact
     4141 atttaaatat ataatgtgaa taaatgttta ataatttgaa atatgataat tttttatgtt
     4201 gaaagcttat aatgattatg atataaattg gttatagtat acaatttatg ggtataacaa
     4261 taaaaaatta gaaaggaatt tttataaaat gattaaatta tatggatata caaaatgctc
     4321 tactgttaaa aaagctaaaa attggcttaa ggaaaataat ttagaatttg aagatataga
     4381 tatggttcaa aaccctccta gtaaagagga gttaaaatca atttataaga ctagtggata
     4441 tgatataaag aaatttttta atacaagtgg tatgaaatat agagaattgg gtcttaaaga
     4501 tattgtaaaa actgaatctg atgataaact tttagaaata ttggtaagtg atggtatgct
     4561 tattaaaaga ccattacttt tggatggtaa aaatgtatta ttgggcttta aagaagatgt
     4621 atggaaaagt actttgctaa aagaagatta ggaagagata ggtgtgttga ttaaaattat
     4681 taattaatac acctataaat ttatgtatta aatctaattt ttataaatat taattagcaa
     4741 attcaaatca aa
//
LOCUS       DAMJYR010000214         4645 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.219, whole genome shotgun sequence.
ACCESSION   DAMJYR010000214 DAMJYR010000000
VERSION     DAMJYR010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4645)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4645)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4645
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.219"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(32..208)
                     /locus_tag="PC471_003675"
     CDS             complement(32..208)
                     /locus_tag="PC471_003675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2790756.1"
                     /translation="MFRDKMDKCTHMLTAYISNSYDYCDFIDTQLDDFILEYGEKVVE
                     SCLHQVMVLVSKYN"
     regulatory      complement(262..351)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            complement(531..731)
                     /locus_tag="PC471_003676"
     CDS             complement(531..731)
                     /locus_tag="PC471_003676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2790757.1"
                     /translation="MPKFTCNLKKYRQLKELTQEQLAEKVGVRRETIMRLEAGKYNPS
                     LKLAIDISKIVDTPIDELFIFD"
     gene            complement(733..1083)
                     /locus_tag="PC471_003677"
     CDS             complement(733..1083)
                     /locus_tag="PC471_003677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3796 domain-containing protein"
                     /protein_id="HDF2790758.1"
                     /translation="MIQKKFFMGFCGFLGFLSLRYFSSGNVADLTYIGFFAFFSNFII
                     AKINGDKADERYVQDEKAAMAFTGQLAIIELFILWCITIVSRNIELMCVLLSITYAIT
                     LNVYAIKLYILEEK"
     gene            1245..1433
                     /locus_tag="PC471_003678"
     CDS             1245..1433
                     /locus_tag="PC471_003678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDF2790759.1"
                     /translation="MTKYDFNFKLKVAKFYLNREGRDILIVKQYGVYDHSQVERWVNI
                     LGESELKIKKFSILYGSS"
     gene            complement(1910..2080)
                     /locus_tag="PC471_003679"
     CDS             complement(1910..2080)
                     /locus_tag="PC471_003679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2790760.1"
                     /translation="MHVYQPKTSEYLLNTYAKSYLSSIVFLFDYITKNESRYAFFIID
                     FPFLSLDIFYKF"
     gene            complement(2469..2957)
                     /locus_tag="PC471_003680"
     CDS             complement(2469..2957)
                     /locus_tag="PC471_003680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HDF2790761.1"
                     /translation="MKNKKGFTLVELLVVIAIIGILAIVALPALFKNIEKAKIAKLEA
                     DISAIKSASLSYYADESKYTEGNIIWWTKKDGKITVNSGIGDEDPLAHKIENLGMPYN
                     GSYTLVSSNGSEEYLELNIIIDGEISKSGLDKLEEDYGSSIKIPNDKNMIITFLSNKS
                     DN"
     gene            3761..4135
                     /locus_tag="PC471_003681"
     CDS             3761..4135
                     /locus_tag="PC471_003681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HDF2790762.1"
                     /translation="MKEEILIEKLLRAELIVMEYLWKKDTLMPKKEIVKEIKQIYGWH
                     KSTIKILLKRLVAKRYLARYIINSQSHYKIIDSKEYYTFKKKVLKSSKSIKIMRSLTT
                     THKNVSKEKLDLLDEYYRNLKE"
     gene            4240..4614
                     /locus_tag="PC471_003682"
                     /pseudo
     CDS             4240..4614
                     /locus_tag="PC471_003682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430377.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
BASE COUNT         1903 a          557 c          545 g         1640 t
ORIGIN      
        1 actaaaaagg gttttctaga aaaaaatctg tctaattata cttacttacc aataccatca
       61 cttgatgcaa gcaagattcc acaacttttt ctccgtactc taatataaaa tcatctaact
      121 gtgtatctat aaaatcacaa taatcgtatg aattactaat ataagcagtt aacatatgtg
      181 tacatttatc cattttatct ctaaacataa tgtatttatc cccctaaaaa actttcatga
      241 aaaacaaaaa gaaaatatct ttttggcaac tggctgacat aacttatttt ataagcgtta
      301 gtccctatag ctttgcgtca ctaaatttct ctagttttgc cgatttagtt ttattctaca
      361 gattaataat acaatagaat tagttatcat tcaacatatc tagttgaata attaataaat
      421 ttatatatga actttaaatt atttgaaaaa ataaaaagcc cctctgtcag gagtgactta
      481 aaaattgaat ttaagtttgt atttaaaaca catttttaat aatgcaaaaa ctaatcaaaa
      541 ataaacaatt catcaatagg tgtatctact atttttgata tatctattgc taatttcaaa
      601 gaaggattat actttcctgc ttctaaacgc ataatggttt ctcttctaac tcctactttt
      661 tctgctaatt gttcttgagt taattctttt aattgtctat atttttttag gttacaagtg
      721 aatttaggca tattattttt cctctaaaat ataaagtttt atagcataaa catttaaagt
      781 gattgcatat gtaattgata gtaaaacaca cattaattct atattcctag acactatagt
      841 aatacaccat aaaatgaata attctataat agcaagctgt cctgtaaatg ccatagctgc
      901 tttttcatct tgtacatacc tttcatctgc tttatcacca tttatttttg ctataataaa
      961 gtttgagaaa aaagcaaaaa atccaatata tgtaaggtca gcaacattgc cagatgaaaa
     1021 atatctaaga cttaaaaatc caaggaaacc acaaaaaccc ataaaaaatt ttttctgaat
     1081 cattttttat cctccttagt aattttaaaa gagatatatt tatatcttta tgatacaaat
     1141 atatctcttt tatattcttt tgtcaataga aaatcagtat tatacaatat ttatactgca
     1201 ccataattgt tagagcaaaa atctaatgct tgagatgttt atgtatgact aaatatgatt
     1261 ttaatttcaa attaaaagta gctaaatttt atttgaatag agaaggaaga gatattttaa
     1321 tagtaaaaca atatggagtt tatgaccatt ctcaagttga aagatgggtt aatatattag
     1381 gagagagtga actaaaaata aaaaaatttt ctatactata tggttcaagt tagatgttat
     1441 aaactatatg atgataacta aatatagtgt acaataaatc gctaagtatt ttggaataaa
     1501 caatagcaca gttatagcaa catgaaaata aaaattttta catagtggtg ttgatgcctt
     1561 ttctaaaatg aaaggaagac cataatgaat aaaaacaaaa acactaaaga aaaaataaat
     1621 taacaagaga acaagaatta gaatatgaaa ataaattttt aaaagatgag cttgagtttt
     1681 taaaaaagat gtgtgtctca gggaagccac attaaaaaac agtggtatca ttattaatca
     1741 aaagaaagtt agatgtatta tcaaaaaatt agaacttaaa tgcatagcat tttttcataa
     1801 atcacataaa tataactcct ataaaggtat aattggaaaa gtaaaaaata ataaaacaaa
     1861 tagaagattt aaaacatcaa tagcatatca gaaaataaca aatgatatgc tagaatttat
     1921 aaaatatatc taatgataaa aatggaaaat caattataaa aaaggcatat ctcgattcat
     1981 tttttgttat atagtcaaat aagaatacta tagatgaaag atacgatttt gcatatgtat
     2041 taagtaagta ttctgatgtt tttggttgat acacatgcaa taaagaagtt gtgtttgcag
     2101 ataaattagc aaaactatag tataagttgc aacaataaag aaaatgaaaa ttatcatact
     2161 aaagcataag gcatgttaca attttattag ataggacatt aacatacact aaagtacttt
     2221 ttattataaa gagtacttat ttttttgaaa ttcattaatg tataaactat caagaatatt
     2281 actcaacata atttaaaaac aacttgaata ggtattttta aattatgtga tataataaaa
     2341 aatagaagtg tatgccaata cacttctgta gttataacta agcattctat tcttgaggag
     2401 tgcttttttc atttttataa ccaactcttt ttactttaac aataattatc agacctatat
     2461 atttattttt aattgtctga tttattagat aaaaaagtta ttatcatatt tttatcattt
     2521 ggtattttta ttgaactacc ataatcttct tctaatttat ctagaccact tttacttatt
     2581 tctccatcta taattatgtt taattctaag tattcttcac taccattaga tgacactaaa
     2641 gtgtacgaac cattataagg catgcctaaa ttttctattt tatgtgccaa agggtcttca
     2701 tcaccaatac cagagtttac tgttattttt ccatcttttt tagtccacca tattatgttt
     2761 ccttcagtat attttgattc atctgcatag tagctaagag acgcactttt tattgcactt
     2821 atatcagctt caagtttagc tatctttgct ttttctatat ttttaaataa tgctggaagt
     2881 gctactattg ccaatattcc tattatagca attactacta ataattccac tagagtaaat
     2941 ccttttttat ttttcattta tttcccctcc atataaaatt aataatatat aaatttttat
     3001 tttgttaatt tctactttct atattttacc atatatagac aaaaaaatca ctttctatga
     3061 caaatatact attattttac aatttatgga aatgatttaa aatgacattt cttgttagaa
     3121 aagatataag aaatggatta aaataaagaa aactacagtc atatcaatgt caataatatt
     3181 atttaaaagc agatgaaaat aaaaagagga ctcagaaaat aatgtctatt taattatact
     3241 tacttaccaa taccataact tgatataaac aagattatac tacatttttt tatgctctag
     3301 tataaaatca tctaactgtg tatctataaa atcaaaataa ttatatgaac tatcagtata
     3361 ataagtcaaa atatgtatac atgaatacat attttaaaag atatatatta ttacatacct
     3421 aaaaaacttt cataaaaaca aaaataaaat atgcttttag taacttgtta acataactta
     3481 taatttattt tataagcatt agcctatata gctttgtgct actaaatctc tttgattcta
     3541 acgatttagt gctattctac aactaaaata atacaataga gctatttatt gtttggtagc
     3601 attgtttgta aattaaacaa atttatacat atgaatatta ttttacattc aaaaaaacag
     3661 actaaatatg tagaatattt ggtaaaactt acctctagtt tttgtcttaa aaagctccta
     3721 ttattaactt aagttagaag aattaaggaa gtgacataaa ttgaaagaag aaatattaat
     3781 agaaaaatta ttaagagcag aattaatagt aatggagtat ttatggaaaa aagacactct
     3841 aatgcctaaa aaagaaattg taaaagaaat aaaacagatt tatggatggc ataaaagtac
     3901 aataaaaata ctactaaaaa ggctagttgc taaaaggtat ttggctagat atattataaa
     3961 ttctcaatct cattataaaa taatagatag taaagaatat tatactttta agaaaaaggt
     4021 actaaaatct agtaaaagca taaaaataat gcgttcactt accactacac ataaaaatgt
     4081 aagtaaagag aagttagacc tattagatga gtattataga aacttaaaag aatgatatag
     4141 taaaaatcga atgtaattat ttaccttaat tcttttgttt gtaaaacttt attgatacag
     4201 aatttaacat atacataaat tggtaaagga atgtatacta tgaaaattag taaattacca
     4261 gaagcggaat taaaagttat gagatatatt tgggaatcag aaaaggtatt aatatcaaga
     4321 gaaatcacca agtctatgga acaaaaatat gaatggaaag atacaactat atttacagta
     4381 ttaaaaagat tactaaagaa gagagttttt agaagcggag aaaatagata agtgtacaca
     4441 ctataatgta ttagtaaaaa aaaataatat cagagatttt aaacaaaaga atttttaaag
     4501 aatacacata aaaattcaat attaagttta atgtcagcac tatgtagtaa ggatgatgaa
     4561 gtagatggtg atattttaaa tttattagaa gaatatttta agaatttgaa ataagaaaat
     4621 gacaaattga tacataataa ataaa
//
LOCUS       DAMJYR010000215         4578 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.220, whole genome shotgun sequence.
ACCESSION   DAMJYR010000215 DAMJYR010000000
VERSION     DAMJYR010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4578)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4578)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4578
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.220"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            231..1310
                     /locus_tag="PC471_003683"
     CDS             231..1310
                     /locus_tag="PC471_003683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HDF2790763.1"
                     /translation="MPELLKNMYNRESLYEVAVAIQSVYNSFKVDEFIKSTMDETWNN
                     LELKARCRKISMSLGMYLPEDYKEALSILEKSVTGFYFAFFFPDFVEVYGQDDINWDL
                     SISALERNTEYWSSEFAVRAFIIKDEERMMAQMRKWSKHKSEHVRRLASEGCRPQLPW
                     GQAISKFKKDPTPVLPILEQLKTDTSTYVQKSVANNLNDISKTHPDLVISIVKDWYGK
                     NKSTNWIVKHGCRTLLKKGNRDVLALFGYDDTTSINLQDFTLETTSISIGEDLTFSFN
                     ILAKKATKTRLEYGIDYIKSNGKRNRKIFKISEVSLKENEKKSYMKKHSFADVSVRKH
                     YPGIHSIAIIINGIEKDKLDFELGI"
     gene            1634..2002
                     /locus_tag="PC471_003684"
     CDS             1634..2002
                     /locus_tag="PC471_003684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HDF2790764.1"
                     /translation="MNVYSFWNDTLAQDAVKIRKYFHEDAYINWHDTNEHFTLEEFII
                     ANCEYPDKWNGKVERVLEFGNLVITVTHVYAINKSLSFHVVSFISLKDDKIISIDEYW
                     GEDDDVPQWRLDKHIGKPIK"
     gene            2119..2922
                     /locus_tag="PC471_003685"
     CDS             2119..2922
                     /locus_tag="PC471_003685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDF2790765.1"
                     /translation="MEEKYILSDRGKVYYWIKESNSVFAKNLIFLPGLTANHHLFDFQ
                     IQYFSKNHNVLVWDAPAHGKSRPYLDFSYSNLVEELKVILDRQNMKKVVLIGQSAGGF
                     VAQSFIVKYPNMVEGLMMIGTCPYGTDYYSKSDIFWLKQTEWMLKLFPDKLLRNIISK
                     MCGATSEGRRSMLKMLEGYEKKELCHLMYMGFAGFIPEIQDLNIHCPVCLVVGDKDKT
                     GKVRKYNEQWHNKTGYPLHIIKNASHNVNVDKPNELNQIMDIFIKQLNI"
     gene            3314..4204
                     /locus_tag="PC471_003686"
     CDS             3314..4204
                     /locus_tag="PC471_003686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HDF2790766.1"
                     /translation="MIESIPAKQILQKVKFDNTRWFGIDYNMNLYRGCSHGCIYCDSR
                     STIYNIENFDKVRYKENVIEILSKELRSKRKKGVVGIGAMSDTYNPFEKQLCITKQAL
                     DLISENHFGVSIDTKSSLVVRDIPILQKIKKNNSAIVKLTITTANDELSKKIEPYVNP
                     SSVRFDAVKELNDSGIFCGILLTPMLPFLTDNKDEIRAIVEKAHKANAKFIYCMYGVT
                     MRSGQREFFYEHLRDISPKLVLKYQKTYGLNYVCTVQNKDSCEKLLREECARYGILTD
                     MKDIIKAYKRSESYTQIKFF"
BASE COUNT         1735 a          493 c          860 g         1490 t
ORIGIN      
        1 cagtaaagta taaaattcta ttactataat aaaagataga actagaagtc tttatgaaag
       61 tgggaaaagt atttatataa ataaattcat atagtttata gtttaatagt aggaaatatc
      121 gtatgagtaa gaatattgta ttttacataa agatttagca atatgacaca ctgttgtcgt
      181 gttctgtagg atatgattaa taaatcagat atgaaaggag agtgagtaat atgccagaat
      241 tactaaaaaa tatgtataat cgtgagtcac tttatgaagt tgcagtagct atccaatcag
      301 tttacaactc atttaaggta gacgagttca taaaatctac aatggatgag acatggaata
      361 acttagaatt aaaagccaga tgtcgaaaga taagcatgag tttaggaatg tatttaccag
      421 aagactataa ggaagctctt tccattcttg aaaaatcagt tacaggtttt tattttgctt
      481 ttttctttcc agattttgtt gaagtttatg ggcaggacga cataaattgg gatttatcta
      541 taagtgcatt agaaagaaat actgagtatt ggtcttcaga atttgctgta agagccttta
      601 tcatcaagga cgaagagcgt atgatggcac agatgcgtaa atggtctaag cataagagcg
      661 aacatgtacg aagacttgca agtgaaggct gtcgtcccca gttgccttgg ggacaagcaa
      721 tatccaaatt taaaaaagac cctacacccg tactgcctat attggagcaa cttaaaacag
      781 atacatcaac gtatgtacaa aagagtgttg ctaataatct aaatgatatc tcaaaaacac
      841 atccagattt agtcataagc attgtaaagg attggtacgg taaaaataaa tccactaact
      901 ggattgtaaa gcatggttgc agaactctac taaaaaaagg caatcgtgat gtattagccc
      961 tttttggata tgatgatact acttctataa atcttcagga ctttacttta gaaacaacgt
     1021 ctatttctat tggagaggat ttaacttttt catttaatat tttagcaaaa aaagcgacaa
     1081 aaacaaggct cgagtacgga attgattata ttaaatctaa tggaaaaagg aataggaaga
     1141 tatttaaaat atcggaggtt tcattaaaag aaaatgagaa aaagtcatat atgaaaaaac
     1201 attcatttgc agatgtaagt gtaaggaaac attatccagg tatacactcg attgctatta
     1261 ttattaatgg tatagaaaaa gataaacttg attttgaatt aggtatataa ttttactaac
     1321 agaataatct atatcagata ctttgctaaa cttatataat atattttact aaatttatat
     1381 taggtagttt actacttgtt tactgaattt aaatacaaag aaatacaggt gctaatccct
     1441 gcccattaaa ggaatatgag cataacacaa aagtagaact tatcaattct gttacgggta
     1501 tagtagtaaa tccatagaat cacaattaga agatgaagtg taaaaaataa gttagtgttg
     1561 attttagttg ctagtgatat tgaatagtta tacaatagcc atataaatta gaatttgtag
     1621 aggagaaata taaatgaatg tatatagttt ttggaatgac actttggcac aagatgctgt
     1681 taagataaga aaatattttc atgaagatgc ttatattaat tggcatgata caaatgagca
     1741 ttttacttta gaagaattta ttattgcaaa ttgtgagtat cctgataaat ggaatggtaa
     1801 agtagaaagg gtgctagaat ttggaaattt agtaattaca gtgacacatg tttatgcaat
     1861 aaataaatca ctatcatttc atgtcgtttc atttatcagc cttaaagatg ataagattat
     1921 ttctattgat gaatattggg gagaggatga tgatgtgcca cagtggagat tagataaaca
     1981 tattggaaag ccaataaaat agtgtttgtt ctgttttata actgaaattt tgataacatt
     2041 tgatattttg atttgtcatt ggtcaacata gtcgttagtg taataattta aaaaagtaga
     2101 atttggagag tgaacactat ggaagaaaaa tacatcttat ctgaccgagg gaaagtatat
     2161 tattggataa aagaaagtaa tagtgtgttt gcaaagaact taattttttt gccaggacta
     2221 acagcaaatc atcatctttt tgatttccag atacagtact tttcaaaaaa tcataatgta
     2281 ttggtatggg acgctcctgc tcatggaaaa tcaagacctt acttagattt ctcatattct
     2341 aacttggtag aagaattgaa agtaatttta gatagacaaa atatgaaaaa ggttgtactt
     2401 atagggcaat cggcaggggg atttgtggca cagtccttta ttgtaaaata tccaaatatg
     2461 gtagaaggac taatgatgat tggaacttgt ccatatggaa ctgactatta tagtaagtca
     2521 gatattttct ggttaaaaca gactgaatgg atgttaaagt tattcccaga taaattgttg
     2581 cgaaacataa tatctaagat gtgtggagct acgtctgaag gcagaaggag tatgctgaaa
     2641 atgttagaag gttacgaaaa aaaagaactg tgtcatctca tgtatatggg atttgctggg
     2701 ttcataccag aaattcaaga cttaaatata cattgtccag tttgtttggt ggttggagat
     2761 aaagacaaga cgggtaaagt gagaaaatat aatgagcagt ggcacaataa gactgggtat
     2821 ccattgcaca ttataaaaaa tgcttcacat aatgtaaatg tcgataaacc taatgaacta
     2881 aatcagatta tggatatatt tattaagcaa ttaaatatat aaaaactaca tactcattag
     2941 gtaaattatg tagtatatgt gtttacagat ggttatagaa ataaatatgt aataaactac
     3001 ttaacactta aaaagtggat ttgtccagag cataatacaa agcatgatat atataaattt
     3061 ttaagcgaaa tacatcaaaa gaaggattag aattagtgga gacagaacag ctattataga
     3121 ttataaataa atagttttaa aggagaggta ctacgaatat agaggaaggt aaatcatacg
     3181 ttattcaagt attataaatg attagtttaa aagtactatc ggataggagt ctccatattt
     3241 aaaccttagt aaaggtagtt aaagtagtag tttaaaaact aaaaaattat ataaaggaat
     3301 taggtataag aaaatgattg agagtatacc agcaaaacaa attttacaaa aagtgaaatt
     3361 tgacaataca agatggtttg gaattgacta taatatgaat ctctatcgtg gttgttctca
     3421 tggatgtata tattgtgata gccgtagcac aatatacaac atagagaatt ttgacaaagt
     3481 tagatataaa gaaaatgtga tagaaatttt aagtaaagaa ttgcgttcaa aaagaaaaaa
     3541 aggtgtagta ggcattggag ctatgtctga cacatataat ccttttgaaa agcaactttg
     3601 tattacaaag caagcactag atttgatttc ggaaaatcat tttggagtgt ctattgatac
     3661 taaaagtagt ttggtggtta gagatatacc gattttgcag aaaattaaaa aaaataattc
     3721 tgctattgta aagttgacta taaccactgc aaatgatgaa ttatcaaaga aaatagaacc
     3781 ttatgtaaat ccaagttctg tacgatttga cgcagtgaaa gaacttaatg attctggaat
     3841 tttttgtggc atattattga ctcctatgct tccatttttg actgataata aagatgaaat
     3901 acgagcaatt gtagaaaaag cacacaaggc taatgcaaaa tttatttatt gcatgtatgg
     3961 tgtaacaatg cgtagtggtc agagagagtt tttttatgag catttaagag atatatcacc
     4021 aaaattggta cttaaatatc agaaaacata tggtttaaac tatgtatgta ctgtacaaaa
     4081 taaagattct tgtgagaagt tgttaagaga agaatgtgca cgttatggta ttttgacgga
     4141 tatgaaagat attataaaag cgtacaagag gtcggagtca tatactcaaa taaaattttt
     4201 ttaagatagg ctttggatga gatataatag tgttgattga tgtgttgata ttatagttga
     4261 ttgttagaga ggatattata gatgaatatt agtattgata ttttaggtaa ttgttagagc
     4321 tgatattata gatgaatatt agtattgata ttctaggtaa ttgctagagt tagtattata
     4381 gatgaatatt agtattgata ctctaggtaa ttgctagagt tagtattata gatgaatatt
     4441 agtattgata ttctaggtaa ttgctagagc tgatattata atttattatt agtattgata
     4501 ttctaggtaa ttgctagagc tgatattata atttattatt agtattgata ttctaggtaa
     4561 ttgctagagt tagtatta
//
LOCUS       DAMJYR010000216         4539 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.221, whole genome shotgun sequence.
ACCESSION   DAMJYR010000216 DAMJYR010000000
VERSION     DAMJYR010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4539)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4539)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4539
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.221"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            174..1313
                     /locus_tag="PC471_003687"
     CDS             174..1313
                     /locus_tag="PC471_003687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HDF2790767.1"
                     /translation="MVIKLTIEEYKNKYFTGNKIVLSINSFDNKELYYHETENILNLI
                     NEIDKLTSSKIFKTIEDKNDLALESYYSSVIEGAFSTRKIAKSIIRGKMKPSNKSEYM
                     IYNNHRALEYGLDNLDELYSHKFIYDLHHILGENCLDSEEYEYRTEKVYVCDSKGEII
                     HTGLEPLKIYDFISKLIDFMENSKVSNLIKSAIIHFYFVYVHPFSDGNGRTSRALSYL
                     YLIDKGYDTFKDFSISYMISKNRTKYYKAILDVENKGNNLTVFIEFMLKSIIQSINEM
                     RNMHDRKSLESILKEELFENDITLSATEEHILKYICNKDNYSMTLENYIKKNKSRYLK
                     AGIKEIELVDQLMEVFNNLEEIEILSKEKNIYKVNDKYLKMLDID"
     gene            1339..1527
                     /locus_tag="PC471_003688"
     CDS             1339..1527
                     /locus_tag="PC471_003688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2790768.1"
                     /translation="MNNKKKAFLISGAINVISLIIVFSLFHYNIIENSTQNMIITVIL
                     IFIFEMIKIKIIEKYYKL"
     gene            2311..4095
                     /locus_tag="PC471_003689"
                     /pseudo
     CDS             2311..4095
                     /locus_tag="PC471_003689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860844.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
BASE COUNT         1951 a          509 c          735 g         1344 t
ORIGIN      
        1 gtaaacttcc tgtaacatat attttaaatg cttattctta aatttttgca ttatttatta
       61 aaatttatta tgctttaacg ctaaattgag tttgataatt ataaaacaaa taatcaaaaa
      121 tagtgtaaaa attaatactt tttgttaata ttaatagata aagataatct aaggtggtga
      181 ttaaactgac aatagaagaa tacaaaaaca aatactttac tggaaataaa atcgtacttt
      241 ctataaatag ttttgataat aaagaactat actatcatga aactgaaaat atattaaatt
      301 taataaatga aatagataaa ttaacatcaa gtaagatatt taaaacaata gaagataaaa
      361 atgatttggc actagagtct tattattcta gtgtaataga aggtgctttt tcaactagaa
      421 agattgcaaa atctataatt agaggaaaga tgaaaccaag taataaaagt gaatatatga
      481 tttacaataa tcatagagcc ttagaatatg gattagataa cttagatgaa ttgtatagtc
      541 acaaatttat atatgatttg catcatatat tgggcgaaaa ctgcttggat tcagaagaat
      601 atgagtatag aactgaaaaa gtatatgtat gtgattcaaa aggtgagatt atacatacag
      661 ggcttgaacc tttgaagata tatgacttta ttagtaaatt aatagatttt atggaaaata
      721 gcaaagtaag taatttaatt aaaagtgcta taattcattt ttactttgta tatgttcatc
      781 cttttagtga tggtaatgga agaacatcaa gagccttatc atatctatat ttgattgata
      841 aaggatatga tactttcaag gatttttcaa tatcatatat gatatctaaa aacagaacta
      901 agtattataa ggctatactt gatgtagaaa acaaaggaaa taatttaact gtatttatag
      961 aatttatgct caagtctata atacaatcta ttaatgagat gcgtaatatg catgatagaa
     1021 aatctttaga aagtatatta aaagaagaat tgtttgaaaa tgacataact ctatcagcaa
     1081 cagaagaaca tatattaaaa tatatttgca ataaagataa ctattctatg acattagaaa
     1141 attatataaa aaagaataaa agtagatact taaaggctgg aataaaggaa attgaattag
     1201 ttgaccagtt gatggaagtg tttaataatc ttgaagagat agagattttg tctaaagaaa
     1261 aaaatatata taaagttaat gataagtact taaaaatgct tgatatagac taatttagat
     1321 aatctggaga gggaatatat gaataataag aaaaaggcat ttttgatttc tggggctata
     1381 aacgtaattt cattaattat tgttttttca ttgtttcatt ataatattat agaaaatagt
     1441 acacaaaata tgataataac tgtaatctta atttttattt ttgaaatgat aaaaataaaa
     1501 atcatagaga aatactataa actataaaca agaaatatct tttctaagag catattgtac
     1561 aatatgctct taatttagtt atatagattt aatataaata atgtattttt tgtagatgat
     1621 ttatgtaaga tttatgacca atttgtgtat gatttgtgtt ctttaatcat gacaatatta
     1681 tattgtaaaa aataaattta ataaaaaatg acttggaatt tatattctag gtctttttta
     1741 tataaaaatt taataagttt caaatgttgg taactttgac agtagaaaac gtctattgag
     1801 ataacgagga ttctaattga ggtggagaga tattagcact taaagtagta tatgcaacag
     1861 atataatctt tacaaaagta atttgaatta aaatgtaaat ataaagaata attaaaaaaa
     1921 tatcgcaatt atttgttttt catatggata aaattggtta tttaatatat aattacaagt
     1981 ataaataata aaatttattt gtaaaaagag tagcactttt aattctaaag gctacttttt
     2041 ttatacaaaa ccctattttt tataacaatt cattaacata aaaatcaaac ttaccttaaa
     2101 atataaattt acaaattttt caatcaataa gaaaatttct ttattcctta cataatgact
     2161 gcaatgtaac aaaataatta caaattaatt atgaaatcat tatagttaga aacctcttta
     2221 aaattagata ttaagtatcc tttagaatta gaacgggaac aaagttaaaa aattaagtaa
     2281 tatcaaatgg acaaggggga agaaaataaa atgaaaatac caaagaagat tgctgcaatg
     2341 cttacagtta caatgatagc tggaagttca acagcaggca tagctagtgc acaaactgtg
     2401 gcaacaaatt taacagggca agaaagatat gaaactgctg ttaaaataag tcaagatgga
     2461 tggaaaaatg ctgatgaggt agtaatcgta aacgactcat ctatagcaga tgctctatct
     2521 gcaacaccat ttgcaaaagc aaaaaatgct ccaatacttt taacaagtaa agataaatta
     2581 aatgataaga cgaaagcaga aatacaaaga ttaaaagcta aaaaagtata cttaataggt
     2641 ggaacttctg tattaagtac aaatatagaa aaagaaataa aagatttaaa aatatctttt
     2701 gagagaatct ctggagctga gagatatcaa acatccttag aattagcaaa gaaattagat
     2761 gctataagtg atgttaagaa aatagctgta gttaatggtg aaaaaggact tgcagatgct
     2821 gtaagtgtag gagcaccagc tgcacaaaac aatatgccag taatacttgc tgattctaaa
     2881 aatggaacag cagttgcaga caaatttata aaggatgctg gaataactca atcttatgta
     2941 gtaggtggag agagttcaat ttctgaagct gttaaaaata aactaccaaa tagtacaaga
     3001 ctaggtggaa cagatagaaa tgatacaaat gcaaaagtaa taaaagaatt ttataaaaag
     3061 acagatttaa aaaatgctta tgtaactaaa gatggtatga ataaacaaga ccaacttata
     3121 gatgcactag cagtaggtgt attaggtgct aaaaatcaat caccagtagt tttagttgga
     3181 aagaacttat cagcttcaca aaaatctcta gttaactcta agagttttga taagataact
     3241 aaagttggtg ggaatggtaa tgagacagca tttaatgaaa tgaagtcatt acaagaagta
     3301 aaaacagttg aagctaaaac aatttctgaa ttaaaatcag ctatagataa agctactgct
     3361 aatgatgtta taaactttaa gccaactagt gaggttaaag aagcatttac aatacaaact
     3421 gataaagcag taacagtaaa cttaaatggt acatatacaa agactgtgac tatcaacatg
     3481 cctaatggtg atgtaaataa ctatgcaaaa gtggatgatg tagttataga tgatgtaaaa
     3541 gatggaactt ttgttaatta tggaaaaata actaacttaa aggttaatga taaaaatgga
     3601 gccaagatag aaaataattc taaaggtgag attggtagtt taacagtagc atctggagca
     3661 agtcaagtaa aagtgactaa tggtggtaag ataactacag ttacaaataa ttctaaaggg
     3721 actactatag ataataaagg tacaataagt agtgttaagg gagataattc tccaacaata
     3781 agtggaaata gtccaagttc taactcttca ggaggaagtt catcaagtgg aggttcttca
     3841 catggaggag gctcatcttc aggaggaagt tcttctaatc aaacatcagt aaataatgaa
     3901 gcagcaaaaa taacaagcgt gcctacacca gcaaaagatg caactagatt aactatgcca
     3961 tcagtatcaa gtggatatac tatagcaata aaaacatcaa gtaatgagag tgttataaaa
     4021 aaagatggaa caataatacc tccaaataca gcaacaacag taaaattagt atttacggta
     4081 acacatacat caagtggtaa aacagcagat acaaaagaat tatctgtaac agtaccagct
     4141 aaatcaacag atgaagaatt acaagcagca ttagacaatg aagtggcaaa aataacaagc
     4201 gttcctgcac cagcaaaaga tgcaactaaa ttaactatgc catcagtatc aagtggatat
     4261 actatagcaa taaaaacatc aagcaataag agtgttataa aggaagatgg aacaataata
     4321 cctccaaata cagaggaaac agtaacattg gtatttacag taacacaaga atcaagtggt
     4381 aaaacagcag atacaggaga aatagatgta gtagtaccag ccaaatcaac agatggggaa
     4441 atacaagcag aagtacaagc tgaggcggat aaaataacaa gtgtaactca gccaacacaa
     4501 gatgcaacaa cactaactat gccaacagta gcaagtgga
//
LOCUS       DAMJYR010000217         4527 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.222, whole genome shotgun sequence.
ACCESSION   DAMJYR010000217 DAMJYR010000000
VERSION     DAMJYR010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4527
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.222"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            78..611
                     /locus_tag="PC471_003690"
     CDS             78..611
                     /locus_tag="PC471_003690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HDF2790769.1"
                     /translation="MTEVIIVNDARIISQIKAGKKELFDTLIKKYYDSIYCYCYRHVD
                     NKMTAQDLTQDVFVTVINNIENYKHYGKFENYLYVIAKNKCKDFWKKKQPIYLESLDK
                     EISNQELSDIENKMFIKEALTKLPELEFEVIILRYYHDLRFKDIAKIMESSVSVTKYR
                     MSRALKKLELYLEGGKL"
     gene            608..1369
                     /locus_tag="PC471_003691"
     CDS             608..1369
                     /locus_tag="PC471_003691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2790770.1"
                     /translation="MNNKEIKRSLQKYKVPKYDSSALEKTIKTIHELTLSNTYKRMNN
                     KDFFISQIGFIRKRTWIMQFLILCTICIFLFSTSADSNSFYNSLSLISIASPLFVLTN
                     IEELTKVYNSSILEIEFSTKNTLHKIIATRMLIFGISDLLFMIIILVSAKQIIDVNIL
                     YIIIYILVPFNLMCIGCMEIINRNKGKHTNYYCMIYGISLVLLIVTLSNFRDNLYQFE
                     AINQWLIVFLVTAVTLIYEIRKTWRHLCSFENIII"
     gene            1407..2273
                     /locus_tag="PC471_003692"
     CDS             1407..2273
                     /locus_tag="PC471_003692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2790771.1"
                     /translation="MELSVERLTKQYNNKIIVDKIDFKLNEGIYGFLGENGAGKTTLM
                     RMICGILSPTSGEVKVDGKNNLDMGEDFRDMIGYLPQNFGYYPDFTAKEFMLYIASLK
                     GLMPSYAKRKTQELLEMVGLSEVINKKIRTFSGGMKQRLGFAQTLLNDPKILILDEPT
                     AGLDPKERVRFRNLISEFSKEKIVILSTHIVSDVEYIADKILIMKKGRFVLTGSIEEV
                     TSKIKDKVWKCVVNENEIDYMSDRYCLANIHHSGGVANLRIVSDIKPHDNACNLKPTL
                     EDLYLYCFNEVL"
     gene            2294..3535
                     /locus_tag="PC471_003693"
     CDS             2294..3535
                     /locus_tag="PC471_003693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2790772.1"
                     /translation="MSTLIKFELKKIIKRKSTIAVILGSLFLSIILFALMPIQEKAND
                     ESGKEYRGLQAIELKKQQSKLNEGYITDKKVKNSIRDYQKMFNNPNNITTDEGGFSNF
                     KPDIYWKFFYPNKAFYNFIIESYSNPNDYNPEIIKSMPLEDGVNFYKFRNEKVNTILN
                     MEYPDKNYTQKEKDFWLSKNKDIEEPYLNGYYQGWNYFLLARDLLIFTIVAVCISTAS
                     VFSGEYQSGADSIILSSKYGKTKVITAKIVSSFLFGTIIFTINTIVSIGILLLSFGID
                     GWNLPLQLTTSSIPYPYTFLQAILLSVLIIYMILILLISFTLLLSSYMKSPFPVLITN
                     IILLFVPIFLKYSETNKLYNQILNVLPSNAISFSFTNYVDYKFGNLIIALPTMIIFTC
                     LVLTVVFLPFIKNNFKKHQVN"
     gene            complement(3759..4331)
                     /locus_tag="PC471_003694"
     CDS             complement(3759..4331)
                     /locus_tag="PC471_003694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDF2790773.1"
                     /translation="MFKKIVAIASSIIVSTICLTGCDFNSNDKKGESSSSKNLDKVTI
                     AEVTHSVFYAPQYAAITKGFFEDEGIKIDLINTQGADKTMAALISGEANVGLMGPEAS
                     IYVYNQNKNDYAVNFAQLTKRDGSFLVARKKMPNFSYNDLKGTEILGGRKGGVPLMTF
                     EYVLKEKGLTIGENLKAGNVNVRTDVQKSV"
BASE COUNT         1760 a          517 c          647 g         1603 t
ORIGIN      
        1 tgtatttata aataattata aaataataaa ttaatttttt tatactgact tttttatagt
       61 ataaatcgtt atatataatg acggaggtga taatagtgaa tgatgctcgg attatatcac
      121 aaataaaagc aggaaaaaaa gaattgtttg atactctaat aaaaaaatac tatgatagca
      181 tctactgtta ttgttatcgc catgtagaca ataaaatgac tgcacaagat ttaactcaag
      241 atgtatttgt aacagttata aataatatag aaaattataa acattatggt aagtttgaaa
      301 actatttgta tgttattgca aaaaacaagt gtaaggattt ttggaagaaa aaacaaccaa
      361 tatatttaga gtcattagat aaggagatat caaatcaaga attatctgat atagaaaata
      421 aaatgtttat aaaagaagct ttgactaaat taccagaatt agaatttgaa gtgataattt
      481 tacggtatta ccatgattta agatttaagg atattgctaa aattatggag tcaagcgtct
      541 ctgtcacaaa gtacaggatg agtagggcat taaaaaaact agaattatat ctggaaggag
      601 gtaaattatg aataataaag aaataaaaag aagtctacag aagtataagg ttcccaaata
      661 tgattcttca gctcttgaaa agactattaa aactatacat gagttaacgc ttagtaatac
      721 ttataaaaga atgaataata aagacttttt tatatctcaa attggtttta taagaaaaag
      781 aacttggatt atgcaatttc taatactatg tacaatatgt atatttttat ttagtacatc
      841 agcagatagt aattcatttt ataatagttt atccttgatt tctatagcat caccactttt
      901 tgtactgaca aatatagaag aattaacaaa agtgtataat agtagtattt tagagataga
      961 attttcaaca aaaaatacat tacataaaat tattgctact agaatgctta tatttggaat
     1021 aagtgattta ttgtttatga ttattatttt ggtatctgca aaacaaatca ttgatgtaaa
     1081 cattctgtat ataataattt atatactagt tccttttaac ctaatgtgta taggatgtat
     1141 ggaaattata aatagaaata aaggtaagca tacaaattat tattgtatga tatacggtat
     1201 atctcttgta ttattaatag ttacactgag taactttaga gataatctat atcaatttga
     1261 agcaataaat caatggctaa ttgtttttct agtcacagca gtaacattaa tatatgaaat
     1321 aagaaaaacg tggagacatc tttgttcatt tgaaaatatt attatctaag taaaaaataa
     1381 gaatacacag tatcaggagg aatagtatgg agttaagtgt agaacgttta acaaaacagt
     1441 ataacaataa aattatagta gataaaattg actttaaatt aaatgagggt atatatggat
     1501 ttttaggaga aaatggagct ggaaaaacaa ctcttatgag aatgatttgc ggaatattaa
     1561 gtcctacatc tggagaagta aaggtggatg gtaaaaataa tttggatatg ggagaagatt
     1621 ttagagatat gattggatac cttcctcaaa actttggata ctacccagat tttactgcca
     1681 aagaatttat gctatatata gcatcattaa aaggattaat gcctagctat gcaaagagaa
     1741 aaacacaaga attattagaa atggtaggat tatcagaggt aattaataaa aaaataagaa
     1801 cattttctgg aggtatgaag caaaggctag gatttgctca aacactactt aatgacccca
     1861 aaattttaat attagatgag ccaacagcag ggctagaccc taaagaacga gttagatttc
     1921 gcaacctaat ttcagaattt tctaaggaaa agattgttat attgtctact catattgtgt
     1981 ctgatgtaga atatattgca gataaaattt taataatgaa aaaaggaaga tttgttttaa
     2041 ctggctcaat agaagaagta acttcaaaga taaaagataa ggtatggaaa tgtgtggtca
     2101 atgagaatga aatagattat atgtctgata gatattgttt agcaaatatc catcattctg
     2161 gtggagttgc aaatcttaga attgtaagtg atataaagcc acatgacaat gcatgcaatt
     2221 taaaaccaac attagaagat ttatatttat attgtttcaa cgaagtacta taaatataca
     2281 taaggagata ggtatgtcta cattaattaa atttgagtta aaaaaaataa taaaaagaaa
     2341 gtctacaata gcagttattt taggaagttt attcttaagc atcattcttt ttgcattaat
     2401 gccaattcag gaaaaagcta atgatgaaag tggaaaagag tataggggtt tacaggctat
     2461 tgagctaaaa aagcaacaat ctaaattaaa tgagggatac ataacagata aaaaagtaaa
     2521 aaactcaatc agagattacc aaaaaatgtt taataatcca aataatatta caacagatga
     2581 aggtgggttt tcaaatttta aaccagatat ttattggaaa ttcttctatc caaataaagc
     2641 tttttataat tttataatag agtcatatag taatccaaat gattataatc cagaaataat
     2701 taaatcaatg cctttagaag atggagttaa tttttataaa tttagaaatg aaaaagtaaa
     2761 tacaatatta aatatggaat atcctgataa aaattacaca cagaaagaga aagatttttg
     2821 gttgtctaaa aataaggata tagaagagcc ttatttaaat ggatattatc aaggatggaa
     2881 ttattttcta ttggctagag atttactaat ttttactata gtagctgtct gcataagtac
     2941 agcttctgta ttttcaggag aataccaatc cggtgctgat tcaataatat tatcttctaa
     3001 atatggaaaa acaaaagtta taacagctaa aatagtaagt tcctttttat ttggtacgat
     3061 tatttttaca ataaatacaa ttgtgtctat tggtatactt cttttatctt ttggaattga
     3121 tggatggaat ttaccattac aacttacaac cagttcaatt ccatacccat atacattttt
     3181 acaggcaata ttactaagtg tgttaattat ttatatgata ttaatactat taattagttt
     3241 tacactttta ttatcatctt atatgaaatc accttttcca gttttaatta caaatataat
     3301 attactattt gtaccaatat ttttaaaata tagtgaaaca aataagttgt ataatcaaat
     3361 tctcaatgtt ttaccatcaa atgctatttc attttcattt actaattatg tagattataa
     3421 atttggtaat ttaataatag ctttacctac aatgattatt tttacatgct tagttcttac
     3481 agttgttttt ttacctttta ttaaaaataa ttttaaaaaa catcaagtta actaaaatat
     3541 atattataaa aagaaggtgt cttaaaatga actttttagc ttattaggca ctcttctttg
     3601 tattaattta aatatatttt agtattagat taaatatgtt ttcatcattt taacaatgaa
     3661 aaagagcttt atctctattt tgagatagct cttataaagt taaatattaa attgataact
     3721 ataaaatatg tagtttgaat ttataatttg tgttataatc atactgattt ttgtacatca
     3781 gttcttacat taacattgcc tgcttttaga ttttctccaa tagtaagacc tttttctttt
     3841 agaacatatt caaaagtcat tagtggaact ccaccttttc taccacctaa tatttcggtt
     3901 ccttttaaat cattgtaaga gaaattaggc atttttttcc tagcaactag aaaactacca
     3961 tctctttttg tcaattgagc aaagttcact gcataatcgt ttttgttttg attatataca
     4021 tatatagatg cttcaggacc cattaaacct acgtttgctt cacctgaaat caaagctgcc
     4081 atagttttat ctgcaccttg ggtatttata aggtcaatct ttataccttc atcttcaaag
     4141 aatcctttcg taatagcagc atactgtgga gcataaaaaa cagagtgagt aacttctgct
     4201 atagttactt tatccagatt tttagacgaa gaagattcac cttttttgtc attagagtta
     4261 aaatcacatc cagttagaca tattgtactt acaatgatag agcttgctat tgcgactatt
     4321 tttttaaaca taataaatac ctcccataag acatacgaat agtatatagt attaaaaatt
     4381 tattgtagtt gttacaaaaa tgtatctatg cttataatat ttctacaaaa acttaataat
     4441 aattttcagc tttatgttta gttttttcag taatgtgtaa tattggataa attatattga
     4501 ttatttataa actaagtaat aaaatta
//
LOCUS       DAMJYR010000218         4396 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.223, whole genome shotgun sequence.
ACCESSION   DAMJYR010000218 DAMJYR010000000
VERSION     DAMJYR010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4396)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4396)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4396
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.223"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            156..1631
                     /locus_tag="PC471_003695"
     CDS             156..1631
                     /locus_tag="PC471_003695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HDF2790774.1"
                     /translation="MDSEFANAVELSYECAPLLESLKEYSEKDIACFDVPGHVKNRGV
                     AILNKYLGESLMKMDINSSPTMDNVSAPNGIIKNAQDLLAQAYMADEAFFITNGTTQA
                     IHAMILSVIKPGEKVLLPRNIHKSVINALILCGGIPIFIQPEFDEKLGISLNITLEKV
                     RTEIEKDCNIKALFFLNPTYYGVCADLESIIELCHKNNVLVLVDEAHGAHFPFHLDLP
                     PSAISLGADMVAVSIHKTGGALTQSSALLLNRDNVSFEKVLQSINMLQSTSASYLLMA
                     SIDGARVNLVENGEKQLSKALNLSRYAKSKLNKIDGIKVLSTEILKQKGVKFIDETKL
                     CINVKELNLTGFEVYDLLYKNFSIQVELGDSYNILALVSIGTNKSDIDRLVKALSIIA
                     KIYRKESTLNEFNMVQINPIIKLNPREAFYAPKESVEINSCIDRICGESIMAYPPGIP
                     IIAPGELITEEIMEYIIFLKNSNAYLTDVQDKNLDRILVIK"
     gene            1718..2131
                     /gene="speD"
                     /locus_tag="PC471_003696"
     CDS             1718..2131
                     /gene="speD"
                     /locus_tag="PC471_003696"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HDF2790775.1"
                     /translation="MKFETLGRHILVEYYNCDENVLKDPRLIENFMNDSALNAKATIV
                     DSVFHHFNPWGVSGAVIIAESHLTIHTWPEYGYAAADFFTCGDIDPWKSFELLEQLLK
                     SERSESTEIPRGLTTKIKKYSKKDLGKITHKPEAV"
     gene            2161..3012
                     /gene="speE"
                     /locus_tag="PC471_003697"
     CDS             2161..3012
                     /gene="speE"
                     /locus_tag="PC471_003697"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HDF2790776.1"
                     /translation="MELWYTEEWTENVRFSIKVNKHLFEGKSQFQRIDVFDSDEFGKF
                     LTIDGLMMVTYKDEFIYHEMITHVPMATNLNIKKVLVIGGGDGGTVRELSRYPQIEKI
                     DMVEIDKMVVDVSKEYMDICSCKLDDKRVSLYFEDGVNFVKCAHDKSYDLIIVDSTDP
                     IGPGEGLFSTDFYKDCYRILTDDGILVNQSESPYFDFNAKEMKRANKKLKQIFPISEV
                     YQAHIPTYPSGHWLFGFASKKLNPVKNQDRNGWEKLSLKTKYYNSDIHLGSFMLPQYV
                     KEMLDEE"
     gene            3002..3880
                     /gene="speB"
                     /locus_tag="PC471_003698"
     CDS             3002..3880
                     /gene="speB"
                     /locus_tag="PC471_003698"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HDF2790777.1"
                     /translation="MKNNFYHMNTFMSMDKNYEESNLIVFGVGFDGTTSNRPGARFAS
                     SSMRKEFYGLETYSPFLDLDLEDYNICDYGDLEISVGSTEQVLKEIYQETYKIVRDSK
                     VPFMIGGEHLVTLPAFKAVHEKYNDIYVIHFDAHTDLREEYNNSKNSHATVIKRIWDI
                     VGDNKIFQFGIRSGTKEEFKFATEEKHTYMEIGGIDTFENIVNMLNGKNIYLTIDLDV
                     LDASVFPGTGTPEPGGVNYREFQEIFKIIKNSNINIVGCDIVELSPDYDTTGVSTVIA
                     CKILRELCLIISDKIK"
     gene            complement(4060..4260)
                     /locus_tag="PC471_003699"
     CDS             complement(4060..4260)
                     /locus_tag="PC471_003699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000809131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HDF2790778.1"
                     /translation="MKNGIVKWFNNEKGFGFISVEGEDDVFVHFSAIQNDGYKTLEEG
                     EKVSFDITQGNRGPQAENVNRI"
BASE COUNT         1735 a          495 c          753 g         1413 t
ORIGIN      
        1 tgtttttgag taacttcaaa aaaacacttg taaaaatgct gagatgttaa tataataaat
       61 atcatgagtt taataaaagt aaaaataaaa aatagaaata ataaataata aatattaaaa
      121 atatttgaat atagagagga gatgaatttg aaatcatgga tagcgagttt gcaaatgcag
      181 ttgaattaag ctatgagtgt gctccattat tagagtcgtt aaaggaatac tcagagaaag
      241 atatagcttg ttttgatgtg cctggacatg taaaaaatag aggtgtagct atattaaata
      301 aatatctagg tgaaagtctc atgaaaatgg atataaattc atcaccaact atggataatg
      361 tatctgcgcc aaatgggatt ataaaaaatg cacaagattt gctagcacaa gcatatatgg
      421 ctgatgaggc attctttata acgaatggca caactcaagc aatacatgca atgattttga
      481 gtgttataaa accaggtgaa aaagtattac ttccaagaaa tatacataaa tcagtaataa
      541 atgcgttaat actttgtgga ggaataccaa tatttataca accagaattt gatgaaaaac
      601 taggtataag tttaaatatt actcttgaaa aagttagaac agagatagaa aaagattgta
      661 atataaaagc attatttttc cttaatccaa cttactatgg agtttgtgca gaccttgaga
      721 gtattataga actatgccat aaaaataatg ttttagtgtt agttgatgaa gctcatggtg
      781 cacattttcc atttcattta gatttacctc cttctgcaat aagtttggga gctgatatgg
      841 tagctgtaag tatacataaa actggaggag cattgacaca atcttcagca ctcttattga
      901 atagagataa tgtaagtttt gaaaaggttc ttcaatcaat caatatgcta caatcaacat
      961 cagcatctta tttgcttatg gcaagtatag atggtgctag agttaatcta gttgagaatg
     1021 gtgaaaagca actgtcaaaa gcattaaatc tatctaggta tgcaaaatct aaattaaaca
     1081 aaattgatgg aataaaagtt ttatctacag aaatattgaa acaaaaaggt gttaaattta
     1141 tagatgaaac aaaattgtgt ataaatgtaa aagaattaaa tttgactgga tttgaagtat
     1201 acgatttatt atataaaaat ttttcaattc aagtagaatt gggagattca tataatatac
     1261 tggcacttgt gtccattgga acaaataaat cagatataga tagattagta aaagcacttt
     1321 ctataattgc aaagatttat agaaaagaat caacattaaa tgaatttaat atggttcaaa
     1381 taaatccaat tattaaactt aatccaagag aagcttttta tgcaccaaag gaaagtgtag
     1441 aaatcaattc atgtatagat agaatttgtg gagaatcaat aatggcatat ccaccaggta
     1501 taccgattat tgctccagga gaattgataa cagaagaaat tatggagtat attattttct
     1561 taaaaaatag caatgcatat cttacagatg tacaagataa aaacttagat agaatattag
     1621 taataaaata aattacttta ttttgcaagg gcaagtattt gttaagtaga gtttatcaaa
     1681 tagaacttat taaaagtata aaggagttgg ggttgaaatg aaatttgaaa cactaggaag
     1741 acatatacta gtagaatact ataattgtga tgaaaacgta cttaaagacc cacgtcttat
     1801 agaaaatttt atgaatgatt cagcattaaa tgcaaaagcg acaattgtgg attctgtatt
     1861 ccaccatttt aatccttggg gggtttcagg agctgttata attgctgaat ctcatttgac
     1921 aatacataca tggcctgaat atggctatgc agcagcagat ttttttactt gtggtgatat
     1981 agacccatgg aaaagttttg aattattaga gcaactttta aagtctgaaa gaagcgagtc
     2041 tacagaaatt ccaagaggct taactacaaa aattaaaaaa tattcaaaaa aagatttagg
     2101 taagattact cataaaccag aagcagtata aaacatacaa gagaaagtga ggattattat
     2161 atggaacttt ggtatacaga agaatggaca gaaaatgtaa gattttctat aaaagtaaat
     2221 aaacatctat ttgaaggaaa gtctcaattt caaaggatag atgtgtttga tagtgatgaa
     2281 tttggaaaat ttctaacaat agatggatta atgatggtaa catataaaga tgaatttata
     2341 taccatgaga tgataactca tgttcctatg gctacaaatt taaacataaa gaaagtatta
     2401 gtcataggtg gaggtgatgg aggtacagta agagaattaa gccgttatcc tcaaatagaa
     2461 aagattgaca tggttgaaat agataaaatg gttgtagatg tatctaagga gtatatggat
     2521 atatgctcat gtaaattaga tgataaaaga gtgagtttat attttgaaga tggtgttaat
     2581 tttgttaagt gtgcacatga taaatcatat gacctgataa tagtagattc aactgaccca
     2641 ataggaccag gtgaagggct tttctcaact gatttttata aggattgtta tagaatactg
     2701 actgatgatg gaattttagt aaatcaaagt gaaagtccgt attttgactt taatgctaaa
     2761 gagatgaaaa gagctaataa aaaattaaaa cagatatttc caatatcaga agtttatcaa
     2821 gcacatatac caacatatcc atctggtcac tggttgtttg gatttgcttc aaaaaaactt
     2881 aatccagtaa aaaatcaaga tagaaatgga tgggagaaat taagtttaaa aactaaatat
     2941 tataatagtg atatacattt aggttcgttt atgttacctc aatacgtgaa ggagatgcta
     3001 gatgaagaat aatttctatc atatgaacac ttttatgagt atggacaaaa attatgaaga
     3061 atctaatctt atagtatttg gtgttggatt tgatggaact acttctaaca gacctggggc
     3121 tagatttgca agtagctcta tgagaaaaga attttacggt cttgagacat atagtccttt
     3181 tttagatttg gatttagagg attataatat atgtgattat ggagatttag aaattagtgt
     3241 tggaagtaca gaacaagtct taaaagaaat ctatcaagag acatataaga ttgttagaga
     3301 ttcaaaggta ccctttatga ttggaggaga gcatttagtt acattaccag cctttaaagc
     3361 agtacatgaa aagtacaatg atatatatgt aattcatttt gatgcccata ctgatttgag
     3421 ggaagaatat aataatagta aaaattctca tgcaacagta attaaaagaa tatgggatat
     3481 tgtaggtgat aataaaatat ttcaatttgg tataaggtct gggacaaaag aagaatttaa
     3541 atttgctaca gaagaaaaac acacatacat ggaaatagga ggaatagata cgtttgaaaa
     3601 tatagttaat atgctaaatg gaaagaatat ttatctaact atagatttag atgtattgga
     3661 tgcatctgtg tttccaggaa caggtacacc agaacctggt ggtgtaaatt atagagagtt
     3721 tcaagagatt tttaagatta taaaaaactc taatataaat atagttggtt gtgacattgt
     3781 agagttaagc cctgattacg atacaacagg tgtatcgaca gttatagctt gtaaaatctt
     3841 aagagagtta tgcttaataa tatctgataa aattaaatag agtattttat ataaatagta
     3901 tatattataa tataagaagt tattttaaaa aagttagttt aagtttgacg cttttctaaa
     3961 gtaacttctt atttttgtaa ttaaaaataa atataataaa caacaaaaaa gagccctcga
     4021 tagctccttt tctaaaatat atatatttaa attaaataat tatattctat ttacgttttc
     4081 agcttgaggt cctctattac cttgagttat atcaaagcta accttttcgc cttcttctaa
     4141 agttttgtat ccatcatttt gtatagctga gaaatgtacg aaaacatcat cttctccctc
     4201 tacagatatg aacccaaatc ctttttcatt gttaaaccat tttactattc cgtttttcat
     4261 tataagaaaa cctccgcatt attaaaaaat acaaactaaa aaaataagtc tgttctatta
     4321 atataacata aattttaaat aaaagcaaat aaaattttta aaaaaattaa ttacttttag
     4381 aatttaataa aaattg
//
LOCUS       DAMJYR010000219         4367 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.224, whole genome shotgun sequence.
ACCESSION   DAMJYR010000219 DAMJYR010000000
VERSION     DAMJYR010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4367)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4367)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4367
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.224"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            169..1767
                     /locus_tag="PC471_003700"
     CDS             169..1767
                     /locus_tag="PC471_003700"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative manganese-dependent inorganic
                     diphosphatase"
                     /protein_id="HDF2790779.1"
                     /translation="MSLLVFGHKNPDTDSICSAISLAHLKNKLGIKATAYALGDIRKE
                     AKYALDYFKVDAPEILDNVRIQVRDLNYDKVVPLTPTSSILEAYNLMDEKNVKTLPVT
                     YDDGTFAGIITMKEIAKNLLHQHFTTIHTSLSNICKNLNGTILVDCGEDIKGRVVTLS
                     FGMETLIETLKEGDIAIVGDRYDSIEYAIDTKVKLLILTNHTEISKDLLALAKINGVS
                     VVSVESDNYKTSNVINQCSFLDSIEATENLITFKEDDYMDEIKEIMLETNFRSYPILD
                     DDNKFLGLVSKGHLLNPSKKNVVLVDHNEYAQSADGIEQANIVEIIDHHKLGGISTDV
                     PMSFRVMPVGCNSTIIYQMYKENNVEIPYEIAGLLLSAILSDTLLFKSPTTTDIDKKA
                     CEELSKIAKVDMEKYAMDMFKCGTSLDEYTIEEIVNMDFKEFNMSGHRVGIGQVFTLD
                     IDSIFAKKDEFLSYINSTDYDKLILAVTDIIKEGSYLIYKAEDSLIANAFGVEGVQGT
                     FAPGVVSRKKQLVPNLTTAIKNFK"
     gene            complement(2006..4051)
                     /gene="bclA1"
                     /locus_tag="PC471_003701"
     CDS             complement(2006..4051)
                     /gene="bclA1"
                     /locus_tag="PC471_003701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-like exosporium glycoprotein BclA1"
                     /protein_id="HDF2790780.1"
                     /translation="MRNIILYLNDDTFISKKYPDKNFSNLDYCLIGSKCSNSFVKEKL
                     ITFFKVRIPDILKDKSILKAELFIHIDSNKNHIFKEKVDIEIKRISEYYNLRTITWND
                     RVSMENIRGYLPVGISDTSNYICLNITGTIKAWAMNKYPNYGLALSLNYPYQILEFTS
                     SRGCNKPYILVTFEDRIIDNCYPKCECLPIRITGPMGPRGATGSTGPTGVTGPTGSTG
                     ATGSIGPTGPTGNTGATGSIGPTGVTGPTGSTGATGSIGPTGVTGPTGNTGVTGSIGP
                     TGATGPTGNTGVTGSIGPTGVTGPTGNTGEIGPTGATGPTGVTGSIGPTGATGPTGEI
                     GPTGATGATGVTGSIGPTGATGPTGATGVTGEIGPTGEIGPTGATGPTGVTGSIGPTG
                     ATGEIGPTGEIGPTGATGPTGVTGSIGPTGATGPTGATGEIGPTGATGPTGNTGVTGE
                     IGPTGATGPTGNTGVTGEIGPTGATGPTGVTGEIGPTGNTGATGSIGPTGVTGPTGAT
                     GSIGPTGATGATGVTGPTGPTGATGNSSQPVANFLVNAPSPQTLNNGDAITGWQTIIG
                     NSSSITVDANGTFTVQENGVYYISVSVALQPGSSSINQYSFAILFPILGGKDLAGLTT
                     EPGGGGVLSGYFAGFLFGGTTFTINNFSSTTVGIRNGQSAGTAATLTIFRIADTVMT"
BASE COUNT         1295 a          781 c          662 g         1629 t
ORIGIN      
        1 tatataataa taatttttat attaaatttg tagtatttat gtaagaaata aattttatat
       61 aatatttttt cactaaattt ttttcttttt acaacaaata ctcactacct tttgatataa
      121 tatttttatc aattattatt atattcaagt tttgaaagga gtattaaaat gagtttatta
      181 gttttcggac ataaaaatcc agatacagat tcaatatgtt ctgcaatttc attagcacat
      241 ttaaagaaca aactaggaat aaaagcaaca gcttatgctt taggagatat cagaaaagaa
      301 gctaaatatg ccttggatta ctttaaggta gatgcacctg aaattttaga taatgttaga
      361 atacaagtta gagatttaaa ttatgataaa gtagtacctt taacaccaac ttcatctata
      421 ttagaggctt ataaccttat ggatgaaaaa aatgttaaaa cactacctgt aacttatgat
      481 gatggaactt ttgctggtat cattactatg aaagaaatag ctaaaaatct acttcatcaa
      541 cactttacaa ctatacacac atcactatca aacatttgta aaaaccttaa tggtactatt
      601 cttgtggact gtggtgaaga tataaaaggt agagtagtca ctttatcatt tggaatggag
      661 actctaatag agactttaaa agaaggagac atagctattg ttggagatag atatgatagt
      721 attgaatatg ctattgatac aaaagttaaa cttttaatac ttactaatca tacagagatt
      781 tcaaaagatt tgctagctct tgctaagata aatggagtgt ctgttgtttc tgttgaaagt
      841 gacaattata aaacttctaa tgtaataaat caatgtagtt tcttagactc tatagaagct
      901 acagaaaatt taattacatt caaagaagat gattatatgg atgaaataaa agagattatg
      961 ctagaaacaa acttcagatc ttatccaata ttagatgatg ataataagtt tttaggttta
     1021 gtttcaaaag gtcatctatt aaacccatct aaaaagaatg ttgttttagt tgaccataac
     1081 gaatatgctc aaagtgctga tggaattgaa caagcaaaca tagttgaaat aattgaccat
     1141 cataaacttg gtggtatttc tactgatgtt cctatgtcat ttagagttat gcctgttgga
     1201 tgcaatagta ctataattta tcaaatgtac aaagaaaata atgttgagat accttatgaa
     1261 atagccgggc ttcttttatc tgctatatta tcagatacat tattatttaa gtctccaaca
     1321 acaactgata tagataagaa agcttgtgaa gaattaagta aaatagcaaa agtagacatg
     1381 gaaaaatacg ctatggatat gtttaaatgc ggtacttctt tagatgaata tactatagaa
     1441 gaaatagtaa atatggactt taaagaattt aatatgagtg gacatagagt tggtattgga
     1501 caagtattta ccttagacat agactctata tttgctaaaa aagatgagtt tttatcttat
     1561 ataaattcga ctgattatga caaactaatt cttgcggtta cagatataat caaagaaggt
     1621 tcttatttaa tctataaagc agaagacagc ttaattgcta acgcatttgg tgttgaaggt
     1681 gttcaaggta catttgcacc aggtgttgta tcaagaaaaa aacaattagt ccctaattta
     1741 actactgcaa tcaaaaattt taaataatat aaaattttta aataacgaaa actgtctcaa
     1801 atctatattt gagacagttt tttattatat atatatatat tgataataaa gttaaataaa
     1861 aatccataaa tttaaaattt ttatgaatat attttattat tattgttatt ttattcattc
     1921 taaaataggt aaaactcaat atataaaaat acttttgtca tttaaataaa gcctaaatag
     1981 tttttaagat tattttagac acgttttaag tcataacagt atcagctatt ctaaatatcg
     2041 tcaaagtagc cgcagttcct gctgattgcc catttcgtat ccctactgtt gtagatgaaa
     2101 aattatttat tgtaaaagta gtcccaccaa ataaaaaacc agcaaaatat ccagaaagta
     2161 ctcctccgcc tcctggctca gtagtaagcc ctgccaaatc ttttcctcct aaaattggga
     2221 ataggatagc gaaagaatat tgatttatac ttgatgaacc tggttgtaat gctactgaaa
     2281 ctgatatata atacacacca ttttcttgta ctgtaaacgt accatttgca tctactgtta
     2341 tacttgaact atttcctatt attgtttgcc aacctgttat agcatctcca ttatttagtg
     2401 tttgtggaga tggtgcattt acgaggaagt tagcaactgg ctgagaggaa ttgcctgttg
     2461 ctcctgttgg acctgttggg cctgttactc ctgtcgctcc tgttgctccc gttggtccta
     2521 tacttcctgt cgctcctgtt ggacctgtta ctcccgttgg ccctatactt cctgtcgctc
     2581 ctgtatttcc tgttggccct atttctcctg tcactcctgt tgggcctgtt gctcccgttg
     2641 gtcctatttc tcctgtcact cctgtatttc ctgtcggacc cgttgctccc gttggtccta
     2701 tttctcctgt tactcctgta tttcctgttg gacctgttgc tcccgttggt cctatttctc
     2761 ctgtcgctcc tgttgggcct gttgctcccg ttggtcctat acttcctgtc actcctgttg
     2821 ggcctgttgc tcctgttggt cctatttctc ctgttggtcc tatttctcct gtcgctcccg
     2881 ttggtcctat acttcctgtc actcctgttg ggcctgttgc tcccgttggt cctatttctc
     2941 ctgttggccc tatttctcct gtcactcctg tcgctcctgt tggacctgtt gctcctgttg
     3001 gtcctatact tcctgtcact cctgtcgctc ctgttgctcc cgttggtcct atttctcctg
     3061 ttggtcctgt tgctcctgtt ggtcctatac ttcctgtcac tcctgttgga cctgttgctc
     3121 ccgttggtcc tatttctcct gtatttcctg ttgggcctgt tactcctgtt ggtcctatac
     3181 ttcctgtcac tcccgtattt cctgtcgggc ctgttgctcc cgttggtcct atacttcctg
     3241 tcactcccgt atttcctgtc ggacctgtta ctcctgttgg tcctatactt cctgtcgctc
     3301 ctgtacttcc ggttgggcct gttactcccg ttggccctat acttcctgtt gctcctgtat
     3361 ttccggttgg gcctgttggt cctatgcttc ctgtcgctcc tgtacttccg gttgggcctg
     3421 ttactcccgt tggtcctgta cttcctgtcg ctcctcttgg tcccattgga cctgtaattc
     3481 taattggaag acactcacat ttaggataac aattatctat aattctatct tcaaatgtta
     3541 caagtatata cggtttgtta caacctctac tagatgtaaa ttcaagaatc tgataagggt
     3601 aatttaaaga taaagctaac ccataattag gatatttatt cattgcccat gcttttatag
     3661 ttcccgtaat atttaaacaa atatagttgg atgtatcact tatcccaact ggtaaatatc
     3721 ccctaatatt ttccatagac actctatcat tccatgttat agttcgtaaa ttataatatt
     3781 cacttattct tttaatttca atatctactt tttctttaaa aatatgattc ttatttgaat
     3841 caatatgaat aaataactct gcttttaata tacttttgtc ttttaatata tctggtattc
     3901 tcactttaaa aaaagtaatc aacttttctt ttacaaaact atttgaacat ttacttccta
     3961 ttaagcaata atctaaatta ctaaagtttt tatctggata ttttttagat ataaaagtat
     4021 catcatttaa ataaagtata atatttctca tttataatcc ctccattttg ggtctatatc
     4081 atactatgtt taaaatacat gcttagttac aatataatta aaatttatag tattaaattt
     4141 ttatataata atgctgattt ctacaaaaaa agaacatgtc attagaaatt tctaatagca
     4201 tgttcttttt cattattata tattacactt taaattattc cttgactggc ttagctggtg
     4261 taaactaaac cagtctttct aaaatattaa gcactttttt cttattatgt tttttaggat
     4321 ttattgtcta tattattgta aaccaaagtt ataaaattgt aaataga
//
LOCUS       DAMJYR010000220         4275 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.225, whole genome shotgun sequence.
ACCESSION   DAMJYR010000220 DAMJYR010000000
VERSION     DAMJYR010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4275)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4275)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4275
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.225"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            295..2394
                     /gene="nrdE"
                     /locus_tag="PC471_003702"
     CDS             295..2394
                     /gene="nrdE"
                     /locus_tag="PC471_003702"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HDF2790781.1"
                     /translation="MNWIELNNQVIIKNEQGKYQLEKDKEALSSYIEEFIQPKLRKFN
                     NLEERLKYLIDEGYYSKEVINQYSIDVIKNIYDMIDKHKFEFQSYMSANKFYQNYALK
                     SNDGKEILETYNDKVLIVALTLGNGDKDLALNLADKIVKQEFQPATPTFLNAGRKRAG
                     EMVSCFLLSVEDSTEGISYAISSSNHLSKIGGGVALNLSKLRASGESIKDIEGAAGGV
                     VGVAKMLEQSFSYFNQMGARQGSGAVYLTVFHPDFELLMDTKKINADEKIRLATLSLG
                     AIIPDKFMELAEKNEVAYAFYPHTVYKKYGVSLDEIEMDKWYDKLVNDSDIRKKEINP
                     RQMLTKIAQMQQESGYPYVVYIDTANREHTLKDVGIIKMSNLCCEIFQYQTPSEIEGY
                     GGKNEWGQDISCNLGSLNIANVMDNKTIESTVETAIRALSFVADSTDIKPVPTVSNSN
                     SKSHSIGLGAMNLHGYLVRENILYTSDDAIDFSNVFFAMVRYYSIKASMKIAIERNQT
                     FEGFDKSEYVKGRNSKVLSKYYEQSYLPKSEKVRALFEGIYIPTKEDWTKLLDEVKEK
                     GIYNAYLMAVAPTQSISYVQNATSSIMPITEPVEVRTYGDSTTIYPMPFLTNDNMLYY
                     QSAYRMDMRKVIDLVATVQNHVDQGISTTLFVTDEKTTRDIARHYIYAYKKGLKSLYY
                     TRTKMTRDTHECLVCSV"
     gene            2407..3378
                     /gene="nrdF"
                     /locus_tag="PC471_003703"
     CDS             2407..3378
                     /gene="nrdF"
                     /locus_tag="PC471_003703"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HDF2790782.1"
                     /translation="MTFNLHKIHNAVNWNREEDGFTQAFWEQNVKQFWLPEEISVSKD
                     IKVWNELSNKEKELYKKVLGGLTLLDTKQGNNGIPSMMSLTENLQRKAVLSFMGTMEE
                     IHAKSYSSIFMTLLSNLEIDELFEWIETEPTLQRKADLVLAQYENTTNQEGLYLSMVT
                     SVFLESFLFYSGFFYPLYLSGQGKMVASGEIISLILRDESLHGKYIGLLAQEIYDSFD
                     KMDKEMLEEKMYSILYSLMENEIEYTNVIYRESGLEKEVVNFLKYNANRALENLGFEK
                     LYTVDAINPIVLNGLSTETKTHDFFSTKGNGYQKGVYEELEDEDFII"
     gene            3454..4080
                     /locus_tag="PC471_003704"
     CDS             3454..4080
                     /locus_tag="PC471_003704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HDF2790783.1"
                     /translation="MKYEWRKRDKELYLPKKNPVIVEIPEQKFIMLKGSGDPNSKEFT
                     EAIGVLYSLAYAIKTMPKKGIVPEGYFDYTVFPLEGIWDKGEKSKGLDVLIKEDLIYT
                     IMIRQPDFVTQDLLDKATDIVKKKKPHNLLEKVRFGSINEGLCVQMLHIGSYDNEPES
                     FDIMNEFCKKNKLIRKSYTHREIYISDARKVSPESLKTVLRFEVEREV"
BASE COUNT         1712 a          456 c          785 g         1322 t
ORIGIN      
        1 cataataagc ttgtattgtt ttatatttga gttgataaaa atttctttaa ataaaaataa
       61 tgtcaattat aaagtaataa aagtttagat ataaaaaaat ttgtgtatat actatatgta
      121 gtatataata gataaagcac tgactatatg tagtgctttt gtttttgaaa aatacttatt
      181 gcatttctat aattatagat taattttggg atttcaactg ataagtacca aaagaaataa
      241 taagcaaatt aagtgaaatt atggcacaag attatgttaa aaggacggta aataatgaat
      301 tggatagagc ttaacaatca agtgataatt aaaaatgaac aaggcaagta ccaattggaa
      361 aaggataagg aagccttaag tagttacata gaagaattta tacagccaaa gttgagaaaa
      421 tttaataacc tagaagaaag attgaagtat ttaatagatg aaggttatta ttcaaaagaa
      481 gtaataaatc agtatagcat agatgttatt aaaaatatat atgatatgat tgataaacat
      541 aagtttgaat ttcaatcata tatgagtgca aataagtttt atcaaaatta tgcattaaag
      601 agcaatgatg gaaaggaaat attagaaaca tataatgata aagtgttaat tgtagctttg
      661 acactaggaa atggcgataa agatttagct ttaaacttgg cagataaaat agtaaaacaa
      721 gaatttcaac cagctacacc tactttttta aatgctggaa gaaaaagggc tggagaaatg
      781 gtatcatgtt ttttactatc tgtagaagat agtacagaag gtatatcgta tgctatatct
      841 tcatcaaatc acttatcaaa gattggtgga ggagttgcac taaatttatc taaacttaga
      901 gcatctggcg aatctatcaa agatatagaa ggtgcagctg gaggtgttgt tggagtagct
      961 aaaatgttag aacaatcatt tagttacttt aatcaaatgg gagctagaca aggttctggt
     1021 gcggtgtacc ttactgtatt ccatcctgat tttgaattgt tgatggatac taaaaagata
     1081 aatgcagatg aaaaaataag gcttgctaca ttatcattag gagctataat accagataaa
     1141 tttatggagt tggcagaaaa aaatgaagta gcatatgcat tttatcctca tactgtgtat
     1201 aagaaatatg gagtttctct tgatgagata gaaatggata agtggtatga taaactggtt
     1261 aatgatagtg atataagaaa aaaagaaata aatcctaggc agatgttgac aaaaatagca
     1321 cagatgcaac aagaaagtgg atatccatat gtagtgtaca tagatacagc taatagagaa
     1381 catacattaa aagatgttgg aattattaaa atgtctaact tatgttgtga gatatttcaa
     1441 tatcaaacac cttcagaaat agaggggtat ggaggaaaaa atgaatgggg acaagatata
     1501 agttgtaacc taggttcttt aaacatagcc aatgtaatgg ataataaaac tatagaaagt
     1561 acagttgaaa cagcaataag agcactgtca tttgtagcag atagcacaga tataaaacca
     1621 gtgccaacgg taagcaatag taacagcaag tctcattcta taggattagg agctatgaat
     1681 ttacatggat acttagtaag agaaaatatt ttatatactt cagatgatgc tatagatttt
     1741 tctaatgtat tttttgcaat ggttagatac tattcaatta aagcttctat gaaaatagct
     1801 atagaaagaa atcaaacttt tgaaggattt gataaatcag aatatgttaa gggaagaaat
     1861 agcaaggttt tatcaaaata ctatgaacaa tcttatctac ctaagtcaga aaaggtaaga
     1921 gcattatttg aagggattta tataccaaca aaagaagact ggacaaaatt attggatgag
     1981 gtaaaagaaa aagggatata taatgcatat ttaatggcag tagcaccaac tcaaagcatt
     2041 agttatgttc aaaatgctac atcaagtata atgccaatta ctgagccagt agaagtaaga
     2101 acatatggag actctactac aatatatcca atgccatttt taacaaatga taatatgttg
     2161 tattatcaat cagcatacag gatggatatg agaaaggtca tagatttagt ggcaacagtt
     2221 caaaaccatg ttgaccaagg tatatcaaca acactgtttg ttacagatga aaagacaact
     2281 agagatatag ctagacatta catatatgcc tataaaaaag gtttgaaaag tttatattac
     2341 acaagaacaa agatgacaag agatactcat gagtgtctag tgtgttcagt atagggagag
     2401 aaatatatga catttaattt acataaaata cataatgcag ttaactggaa tagagaagaa
     2461 gatggattta cacaagcttt ttgggaacaa aatgtaaaac aattttggct tcctgaagaa
     2521 atatctgttt ctaaagacat aaaggtatgg aatgaactaa gtaataaaga aaaggaatta
     2581 tataaaaaag tattgggtgg tttaacatta ctggatacaa aacaggggaa taatggaata
     2641 ccatctatga tgagtttaac agaaaaccta caaagaaaag ctgtcttgtc ttttatgggg
     2701 actatggaag aaatacatgc aaaaagttat tcttctatat ttatgactct actttctaat
     2761 ttagaaatag atgaattatt tgaatggata gaaacagaac caacattaca aagaaaggct
     2821 gatttagttt tagctcaata cgaaaatact acaaatcaag agggattata cctatcaatg
     2881 gttacaagtg tatttttaga gagtttcttg ttttattcag gattctttta tccattgtat
     2941 ctatctggtc aaggaaagat ggtagctagt ggagaaataa tatcgcttat tcttagagat
     3001 gagtctttac atggtaagta tataggattg ctagcacaag aaatatacga ttcatttgat
     3061 aaaatggata aggagatgtt agaagagaag atgtattcta tactttatag ccttatggag
     3121 aatgagatag agtatacaaa tgttatatac agagaaagtg ggcttgaaaa agaggttgtg
     3181 aactttttaa aatacaatgc gaatagagct ttagaaaatc tagggtttga gaagctatat
     3241 actgtagatg ctataaatcc aattgtatta aatggtctta gcactgaaac caaaacacat
     3301 gatttttttt caactaaggg taatggatac caaaaaggag tgtatgagga attagaggat
     3361 gaagatttta taatataatt tataattatg acacactgtt gtcactttta gcgtgataat
     3421 atcaatgtat aaaatatata tggaggtatt tttatgaaat atgaatggag aaaaagagat
     3481 aaagagttat atttacctaa aaagaatcct gtaatagttg aaataccaga acagaaattt
     3541 atcatgttaa aagggagtgg agacccaaat tctaaagaat ttactgaagc aataggagta
     3601 ctttattctt tggcatatgc aataaaaacc atgcctaaaa aaggaattgt accagaaggg
     3661 tattttgact atacggtttt tcctttagaa ggtatatggg ataagggaga aaaaagtaaa
     3721 ggcttagatg tacttattaa agaagattta atttacacta taatgatacg tcagccagat
     3781 tttgttacac aagacctgtt ggataaagct actgatatag taaagaagaa aaaacctcat
     3841 aatcttcttg aaaaagttag atttgggtct attaatgaag ggctatgtgt tcaaatgctt
     3901 catataggtt catatgataa tgaaccagaa agctttgaca ttatgaacga attttgcaaa
     3961 aaaaataaac ttataagaaa atcttacaca catcgtgaaa tttatataag tgatgctaga
     4021 aaagtatcgc ctgaatcatt gaagacggtt ttacgttttg aagtagaaag ggaagtataa
     4081 atattttgaa taatttataa gagttataaa aagtgaagtc tgttgagact tcattttttg
     4141 atgcaatttt aaaattaaat ttgatgtttc ataatatttt aaaaactttc tacttttatt
     4201 agtgtgaata actgttgcta ttaaataata atactaatta tataagtatt attatataat
     4261 atttatagat tatta
//
LOCUS       DAMJYR010000221         4234 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.226, whole genome shotgun sequence.
ACCESSION   DAMJYR010000221 DAMJYR010000000
VERSION     DAMJYR010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4234)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4234)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4234
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.226"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            76..1818
                     /locus_tag="PC471_003705"
     CDS             76..1818
                     /locus_tag="PC471_003705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloendopeptidase"
                     /protein_id="HDF2790784.1"
                     /translation="MVKSKKKIFVFLILIAVFIIFASYIRNYTNDTKKDSVRGTIPFE
                     KMEYKRPNIQSICENINKCNDKLLTAKTAKEQLNLFNEVDKIYQDFYSTLTIAKIRNN
                     IDSSDEFYSKEYKYLMSKSVDVDMSYKDFQDRFVTSKFSKELEKELGKDTFNYLKNIG
                     KLNSKEVEGLLKKEQDLVVKYEDLLAKSTVSIDGIEVDFEEALSRPNLSPEEYVKIYS
                     DYLKKYNPIFGNIFLELIQTRTEIASKQGFKNYIDYAYMNLNKDYSQEEAKKFRQDVK
                     DYIVPLYREISSKPSDSSIYIKVYKNRSFRKFDKVLEDISPKLKESFDYMKKYDLYDY
                     SSGKNKSPGGYTTYINKYKAPFLFNTWDNSFLGVTSFAHEFGHFYNYYNSINLNNKMQ
                     PSIDVCEVHSTSLEILFYKYFDDFFGKQSEAIKKEHLSIVLNTIIDACLYDEFQEIIY
                     KNPYMSLNDINKLFFDLEEEYGVSNNILREKNAPFWILVSHNFQVPFYYLSYGLASDV
                     SLQIWQLSQEDYRKAVDVYMDFLNQNTDAGFKDVVEKVNLQLPFQDGNLEEISSTLYD
                     YFGIDNPLELKNAS"
     gene            complement(1988..2176)
                     /locus_tag="PC471_003706"
     CDS             complement(1988..2176)
                     /locus_tag="PC471_003706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2790785.1"
                     /translation="MKIITIGSSLITVLLFLSTMICGFWIKNNKVTDASSIKFHMNSA
                     IFTGIFLLISTILLIIYI"
     gene            complement(2173..2754)
                     /locus_tag="PC471_003707"
     CDS             complement(2173..2754)
                     /locus_tag="PC471_003707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDF2790786.1"
                     /translation="MKSNDKDARERIIEVTLNLLNEVDDIEEITVRKIAERANVGVGL
                     INYHFKTKDNLLSTAIGDVMSNIIAELYDDSVYTLRPIEDLKNLLKKLCDTGLHYEKV
                     LPFVLNQCITNGDMQAELDIVPMLRKIFGNKKDEMSLRIIALQIILPIQISALSTESF
                     QLYSGINIKNKYERDKFIDILIENIIGEDVDVR"
     gene            2922..3740
                     /locus_tag="PC471_003708"
     CDS             2922..3740
                     /locus_tag="PC471_003708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDF2790787.1"
                     /translation="MFKIGDFSKLSKISIRMLRHYDEIGLLTPSHTNKTNGYRYYSAD
                     QLSTTNRIHALKDMGFGLYSIKEILTEYNDKESLIKYLNIHHSQVKEQLEDTQKKLLK
                     IETTIKRIGGNDIMKNYDVTIKNFAPKYMMTLRRVIPTYQDEGMLWHQAFLETKDQNV
                     QIEPPKYSKAVFYDTGYKEDYVDVEVQVAVSGKYKDTEHVKFKTVPSVTAATAIVNGN
                     FNQVADACEAIGNWISDNNYDVDGPMFNIYHVSPGNDSNPDNWVTEVCFPVKKK"
BASE COUNT         1606 a          534 c          573 g         1521 t
ORIGIN      
        1 attttcttaa catgcatttt ttatatttaa tatagtaaaa taattaataa ctatatccat
       61 gattggagga tttttatggt taaatccaag aagaaaatat ttgtttttct aattttaatt
      121 gcagttttta ttatatttgc atcatacata agaaattata ctaatgatac aaaaaaagat
      181 tctgttagag gaactattcc ttttgaaaaa atggaatata agcgtcctaa tatacagtca
      241 atttgcgaaa acattaacaa gtgcaacgac aaacttttaa cagctaaaac agctaaggaa
      301 caattaaatt tatttaatga agttgataaa atttatcaag atttttacag tacacttact
      361 atagcaaaaa ttagaaataa catagattca tcagacgaat tttactctaa ggaatataag
      421 tatcttatga gtaaatcagt agatgttgac atgtcataca aagactttca agatagattt
      481 gtaacttcta aattctctaa agaattagaa aaagaacttg gaaaggatac atttaattat
      541 ctaaagaaca ttggaaaact taattctaaa gaagttgaag gtttgcttaa aaaagagcaa
      601 gacctagttg taaaatatga agacttatta gctaaatcta cagtatcaat agatggtata
      661 gaggtagact ttgaagaagc cttgtcaaga ccaaatctta gccctgaaga atatgtgaaa
      721 atttattcag attacttaaa aaaatacaat cctatatttg gtaacatatt cttagaactt
      781 atacagacaa gaactgaaat agcaagtaag caaggattta agaattatat agattatgcc
      841 tatatgaacc taaacaaaga ctattctcaa gaagaagcta aaaaatttag acaagatgta
      901 aaagattaca tagttccatt atatagagag atttcatcta aaccatctga ttcttctatt
      961 tatattaaag tatataaaaa tcgctcattt aggaagtttg ataaagtact tgaagatata
     1021 tctccaaaac taaaagagag cttcgactat atgaaaaaat atgacttgta cgattattct
     1081 tctggaaaaa acaaatcacc tggaggatat actacttata taaataaata taaagcacct
     1141 ttcttgttta atacatggga taattctttt ttaggtgtta ctagttttgc tcatgagttt
     1201 ggtcattttt ataattacta caacagtatt aacttaaaca ataagatgca accaagtata
     1261 gatgtatgtg aagttcattc aacatcttta gaaattctat tttataaata ttttgatgat
     1321 ttttttggta aacaatctga agctataaaa aaagaacact tatcgattgt tttaaatact
     1381 attattgatg cttgtcttta tgatgaattt caagaaatta tatacaagaa tccatatatg
     1441 agtctaaatg atatcaataa attgtttttt gatttagagg aagaatatgg agtttccaat
     1501 aatatactta gagaaaaaaa tgctcctttt tggatacttg tatctcataa cttccaagtt
     1561 cctttttatt acttgagtta tggtcttgca tctgatgtat cacttcagat atggcaatta
     1621 tcacaagaag attacagaaa agctgttgat gtatatatgg attttttaaa tcaaaatact
     1681 gatgctggat ttaaagatgt ggtagaaaag gtaaatttac aattaccttt ccaagatgga
     1741 aatttagagg aaataagctc tactttatat gattattttg gtatagataa tccgcttgaa
     1801 ctaaaaaatg cttcttaatt tctaatactt tatactcact cttaagattt agtattagat
     1861 tgaaataagt gatatttttt cacacacaat taatgtttaa taaaattata tggtaaagta
     1921 tcagaaattt ttaagactct tgtctttata atttctgata ctttatttat taataatatt
     1981 attattttta tatatatata attaaaagga ttgtggaaat caataaaaat atccctgtaa
     2041 aaatagcact attcatatgg aacttaatag aacttgcatc tgttacctta ttattcttaa
     2101 tccagaaccc acaaatcata gttgaaagaa aaagtaaaac agtgataaga cttgaaccaa
     2161 tagttataat tttcatctta catcaacatc ctctccaata atattttcta ttaaaatatc
     2221 aatgaattta tctcgctcat atttattctt tatatttata ccactgtata actgaaatga
     2281 ttctgtagat aaagcagaaa tctgtattgg taaaataatt tgcagggcaa taattcttag
     2341 actcatttca tcttttttat ttccaaaaat tttacgaagc atagggacaa tatcaagttc
     2401 tgcttgcata tctccattgg ttatacattg attcaataca aaaggaagca ctttttcata
     2461 atgtaatcca gtatcacaca atttttttaa taaatttttt aaatcttcaa taggtcttag
     2521 agtatataca ctatcgtcat atagctctgc tattatattt gacatcacat ctcctatggc
     2581 agtactcaac aaattatcct ttgttttaaa atgataattt attaaaccaa ctccaacatt
     2641 tgcacgctca gcaatttttc taactgtaat ctcctcaata tcatctactt catttaaaag
     2701 attcaatgta acttcaatta tacgttctct agcatcttta tcattacttt tcataataga
     2761 cctccaaaac aattaacatt ttcagttctg aacatgttca gtataatatt tttttcatca
     2821 tatgtcaata taatttaatt ataattttag ttgactctaa cattatgata tggtttatat
     2881 taatattaag ctaaaagtac ctttataaag gagacaaaaa catgtttaag ataggcgatt
     2941 tttcaaagct aagtaaaatt agtattcgta tgttacgtca ttatgatgaa ataggacttt
     3001 taactccttc tcatacgaat aaaactaatg gatatagata ctatagtgca gaccaacttt
     3061 ctactacaaa cagaatccat gcactaaaag atatgggctt tggcttgtat tctataaaag
     3121 aaattttgac agaatataac gataaagaaa gtctcataaa atatctaaat atccaccatt
     3181 ctcaagtaaa agaacaatta gaagatacac agaaaaaact attaaaaata gaaaccacta
     3241 ttaaaagaat tggaggaaat gatattatga aaaattatga tgtaactata aagaattttg
     3301 caccaaaata tatgatgaca cttagaaggg ttataccaac ttatcaagac gaaggaatgt
     3361 tatggcatca agcattttta gagacaaaag accagaatgt tcaaattgaa cccccaaagt
     3421 attctaaagc agttttttat gatactggat ataaagaaga ttatgtagat gttgaagtac
     3481 aagttgctgt atctggaaag tataaagata ctgaacatgt taaatttaag actgttccaa
     3541 gtgtgactgc tgcaactgca attgttaatg gaaatttcaa tcaagttgca gacgcttgtg
     3601 aggctattgg taattggata tcagataata actatgatgt ggatggtcct atgtttaata
     3661 tttatcatgt aagtcctggc aacgatagta atcctgataa ttgggtaaca gaggtatgct
     3721 tcccagttaa aaagaagtaa tttttatatt aattggataa tattttaatt tgttagacaa
     3781 attactttta gtgttatgta taaataccta caatttaatt aggatattat cttaatttgt
     3841 taaacaaatt acttttagtg ttatgtataa atatctacaa tttaattagg atattatctt
     3901 aatttgtcag ataaatcact tgtgatgtta tgtttgaatg actagaattt aatcaggata
     3961 ttatcttaat ttgtcagata aatcacttgt gatgttatgt ttaaatgact agaatttgat
     4021 tgggatatta tcttaatttg tcagataaat caattgtgat gttgtgttta aatgactaga
     4081 atttgattag gatattatct taatttgtca gataaattat ttataatgtt atgtttaaat
     4141 acctacaatt taattaggat attatctcaa tttgtcagat aaatcaatta tgattttatg
     4201 tttaaatgac tagaatttga ttaggatatt atct
//
LOCUS       DAMJYR010000222         4111 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.227, whole genome shotgun sequence.
ACCESSION   DAMJYR010000222 DAMJYR010000000
VERSION     DAMJYR010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4111)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4111)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4111
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.227"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            3..401
                     /locus_tag="PC471_003709"
     CDS             3..401
                     /locus_tag="PC471_003709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDF2790788.1"
                     /translation="MIKKIEHVVITNNNIENYINFYKKFGFSSKEMSKNYKLNARNVK
                     INLNIKGYKFLPYVKNSKASRDNLCFKINTNIDEPKKYLEQNEIQIKRGIVECTGVFG
                     NMKSIYLKKTNGNLIELSNHRITESKPWKI"
     gene            389..1327
                     /locus_tag="PC471_003710"
     CDS             389..1327
                     /locus_tag="PC471_003710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HDF2790789.1"
                     /translation="MENLILSINVVLPLFLTMSLGYVLKKLGLFDTHTLNKMNNVCFK
                     SFLPLLLFFNIYNTDLDGSLNFKLMAVAVIIIFSIFLILVFIIPKIEKDNKTRGALIQ
                     GVFRSNFVIFGIPVTISLFGDKSIGVTSLLIAVIVPMFNVLSVVILEIFRGGKVNIKH
                     MIKSVLTNPLIIASFIGLTMLILNINIPTFLHKTIGDISKIATPLSLIILGGSFSFSA
                     IKGHIKPIILGVCGKLILVPAIFLPISIFLGFRNVELASLMIMLSAPTAVSSFTMAQQ
                     MDSDSELAGQIVVFTSGFSIITVFLIIFILKQNNLI"
     gene            complement(1711..1935)
                     /gene="secG"
                     /locus_tag="PC471_003711"
     CDS             complement(1711..1935)
                     /gene="secG"
                     /locus_tag="PC471_003711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HDF2790790.1"
                     /translation="MSNILMGVQVVLGILLVISIMPQDSKNALPSEFGGEGSQAYFKP
                     KGKQAFLARVTKITAVLFFINALALLIVKK"
     gene            complement(2528..3820)
                     /gene="eno"
                     /locus_tag="PC471_003712"
     CDS             complement(2528..3820)
                     /gene="eno"
                     /locus_tag="PC471_003712"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HDF2790791.1"
                     /translation="MSVIELVYAREVLDSRGNPTVEVEVVLEDGAMGRAIVPSGASTG
                     AFEAVELRDGDKGRYLGKGVETAVANVNEIIAPEIEGMDAFDQPAIDAIMIELDGTPN
                     KGKLGANAILGVSMAVARAAADEIGLPLFQYLGGVNAKQLPVPMMNILNGGEHADNNV
                     DVQEFMILPVGACCFKEGLRMGAEVFHSLKKVLGEKGLACGVGDEGGFAPNLGSNREA
                     LELIVEAITKAGYKPGEDVMLGLDVAATEMYNKETKKYVLAGEGKELTAAEMVALYED
                     WSNNFPIITIEDGLDEEDWDGWKLLTEKLGNKLQLVGDDLFVTNTERLEKGIENGVAN
                     SILVKVNQIGTITETLDAIEMAKRAGYTAVISHRSGETEDSTIADLAVAVNAGQIKTG
                     APSRTDRVAKYNQLLRIEEMVGEQARYCGLKSFYNLKK"
BASE COUNT         1424 a          661 c          457 g         1569 t
ORIGIN      
        1 ccatgataaa aaaaatagaa catgtggtca ttactaataa taatatagaa aattatatca
       61 atttttataa aaaatttggt ttttcatcta aagaaatgag caaaaattat aaacttaatg
      121 ctagaaatgt taagataaat cttaacataa aaggttataa atttttacct tatgttaaaa
      181 attcaaaagc aagtagagat aacttatgtt ttaaaataaa cacaaatatt gatgagccaa
      241 aaaaatactt agaacaaaat gagattcaaa ttaagagagg aattgtagaa tgtacaggtg
      301 tctttggtaa tatgaaatct atttatttga aaaaaactaa tggaaattta attgaattat
      361 caaatcatag aatcacggag agtaaacctt ggaaaattta atcctatcaa taaatgttgt
      421 tcttcctttg tttctcacaa tgtcacttgg atatgttcta aaaaaattag gtctatttga
      481 tactcatact ttaaataaaa tgaataatgt ttgcttcaag tctttcttgc cattattatt
      541 attttttaat atatacaata cagatttaga tggttctctt aattttaagc ttatggctgt
      601 agctgttata attatttttt caatcttctt aatcttagtc tttattattc ctaaaattga
      661 aaaagataat aagacaaggg gtgcattaat acaaggtgta tttcgtagca atttcgttat
      721 atttggtatt cctgtgacaa tttcactatt tggcgataag agtattggag tcacatcttt
      781 attaattgca gttattgtac ctatgtttaa tgtattatct gttgtaattc ttgaaatttt
      841 tagaggtggc aaagtaaata taaaacatat gattaaaagc gtcttaacaa atccactcat
      901 aatcgcctct tttataggat tgacaatgct aattttaaat attaatatac ctacattttt
      961 acacaaaact attggtgata tttctaaaat agctactcct ttatcactta taattctagg
     1021 cggctctttt agtttttctg ctataaaggg gcatataaaa cccattatat tgggcgtatg
     1081 tggaaaactt atacttgtac ctgctatatt tttaccaatt agcatttttc ttggatttag
     1141 aaatgtagaa ttagcttcac ttatgataat gttatctgct ccaactgcag tttcttcatt
     1201 tacaatggca caacagatgg attctgacag tgaattagct ggacaaattg tagtatttac
     1261 ttctggattt tctataatta cagtattttt aataatattt attttaaagc agaataatct
     1321 tatttaatga tatttgataa cacaaattgg tattataaga aacatatata aaactgattt
     1381 aatcgcgtaa aaacatatta ttatactatt tttactatat aatataactt tgtgtatatt
     1441 atctcattaa acatggtgaa agtagtgttg ctatatattt tttagtttga agaaaatata
     1501 taaatacaaa caatatataa atacaaataa taaacgccac caaatagtca atctataaaa
     1561 taaaaactgg actaatcata taaaatcatt agtccagctt ttattttacg tactttttat
     1621 gttgatgtac ttttattaaa aactaaattt aatttttaaa tgtctagcaa atgcactaat
     1681 taaataatgg ccagttattc aacaatatca ctacttctta actataagta atgccaatgc
     1741 atttataaaa aataatactg ctgttatctt tgtaactcta gcaagaaatg cttgttttcc
     1801 ttttggttta aagtaagctt gacttccttc tccaccaaat tctgaaggta aagcattctt
     1861 agaatcttga ggcattatgc taattactag taaaatccct agtactactt gcacacccat
     1921 taaaatgttg ctcattcttg cccctcctat ctgtttatta cttaaatttc attactcttt
     1981 agtgtatcac aataatatag ccatttcaag actattatgc tataaataac atacaaaaaa
     2041 atatattgtt actataatat attactatat tctcaatata aaaatgaata aaacttagaa
     2101 gagaaaacta tttaaattaa tacataaatt aacacttaaa ataatctact ctcattgtat
     2161 atattataaa tagccaccaa aataaaacaa tcattttaaa atattgatat aacttttaat
     2221 ttcaacataa tttaaaataa ataatccaca tttagcaata atacttcaat gacaattatg
     2281 attaaatcaa aatatgtctt cacttttatt ttttaataaa aacctcctat gcaattagat
     2341 aaaaatgaga tagaaacatt tattcatatt ttttctgaat ctatgaataa aattaagtaa
     2401 taaaatataa aaaaggataa gtgaattgaa aaatcaatta acacttatct cttttatatc
     2461 ttataagatg agcttaaata tcttatattc tctcttcatt aatctttttt agtaaccatt
     2521 ttaataacta tttttttaag ttatagaaag attttaatcc acaatatcta gcttgctctc
     2581 caaccatttc ttctattctt aataattggt tgtacttagc aactctatca gttcttgatg
     2641 gagcaccagt ttttatttga ccagcattta ctgcaacagc taaatctgct atagtagaat
     2701 cttcagtttc tcctgatctg tgagatataa cagcagtata tcctgctctc ttagccattt
     2761 ctatagcatc taaagtttca gttattgtac ctatttgatt aactttaact aatatagagt
     2821 tagctactcc attttcaatt cctttttcta atctctctgt gttagtaaca aataagtcgt
     2881 caccaactaa ttgtaattta tttcctaatt tttcagttaa taatttccat ccatcccaat
     2941 cttcttcgtc taaaccatct tctatagtta ttattgggaa gttgtttgac caatcttcat
     3001 ataaagcaac catttcagca gcagttaact cttttccttc tcctgctaaa acgtattttt
     3061 tagtttcttt gttgtacatt tctgtagcag caacgtcaag acctaacata acgtcttcac
     3121 ctggcttata tccagctttt gttatagctt caactattaa ttctaaagct tctctgtttg
     3181 aacctaagtt tggagcaaat ccaccttcgt cacctacacc acaagctaat cctttttctc
     3241 ctaaaacttt ctttaacgaa tggaatactt ctgctcccat tcttaaacct tctttgaaac
     3301 aacaagctcc tactggtaat atcataaatt cttgaacatc aacgttgtta tcagcatgct
     3361 ctccaccgtt taatatgttc atcattggaa caggtaattg tttagcattt actccaccta
     3421 ggtattggaa taatggtaaa cctatttcat cagcagctgc tctagctact gccattgaaa
     3481 cacctaatat agcattagct cctaatttac ctttgtttgg agttccatct aattctatca
     3541 tgattgcatc tatagctggt tggtcaaatg catccattcc ttctatttca ggtgctatta
     3601 tttcatttac gttagcaaca gctgtttcta caccttttcc taagtatctt cccttatctc
     3661 catctcttaa ttcaacagct tcaaaagctc ctgtagatgc tcctgatgga actattgctc
     3721 ttcccattgc tccatcttct aatactactt caacttctac agttggattt cctcttgagt
     3781 ctaatacttc tctagcatat actaattcaa taactgacat ttgtatatct cccttcgatt
     3841 ttctattagt taaattttca tttactgtaa acatccgtct actattttaa tctactttct
     3901 atattatagt acataaagtt gcttttgaaa attaggtaca aatttatttg atgggtaaat
     3961 ttgtaaaagt tcctattttt attttatttt ttataatatt ttaatttata taatataaaa
     4021 aaacttgtct aaaatccaaa accaatctta aatacacaaa tattataact agatttaaaa
     4081 cattaaacat aaaatgtaca actaatgatt c
//
LOCUS       DAMJYR010000223         4083 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.228, whole genome shotgun sequence.
ACCESSION   DAMJYR010000223 DAMJYR010000000
VERSION     DAMJYR010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4083)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4083)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4083
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.228"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(200..583)
                     /locus_tag="PC471_003713"
                     /pseudo
     CDS             complement(200..583)
                     /locus_tag="PC471_003713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431032.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
     regulatory      809..906
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            961..1137
                     /locus_tag="PC471_003714"
     CDS             961..1137
                     /locus_tag="PC471_003714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2790792.1"
                     /translation="MFGNKMDKCTHVLTAYISSSYDYCNFLDTQLDDFILEYGENVVE
                     SCLHQVMVLVSKYN"
     gene            1363..1563
                     /locus_tag="PC471_003715"
     CDS             1363..1563
                     /locus_tag="PC471_003715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003723180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HDF2790793.1"
                     /translation="MNNGIVKWFNNEKGFGFISMEGRDDVFAHFSAIQTSGFKSLEEG
                     QQVSFDIVKGARGPQAENITIL"
     gene            2568..3413
                     /locus_tag="PC471_003716"
     CDS             2568..3413
                     /locus_tag="PC471_003716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HDF2790794.1"
                     /translation="MNIIKSITLLLTLLIVILMTGCSPRESASEVAKKYIDKMNSGDY
                     ETAYSLLSKDSKKKISIENLEEFQNILVSTKRIISYKIGKEKIIKKYKHDGKEYKNVV
                     KLEETFNIKNLLYKKDDSLKVNRYFVVENNKYKLLLYESFKKEFGINSMDLAQLKLND
                     FGLKYFKEVDLILKKAIAINPTDSKLYYAQACNYMNFDGFPGYSDTTYNALDSINKSL
                     KYLDKNDSDYKKNASNIYNLKGVILMFHKRFDEAQQYLNKALYFNKDNEDPKYNLNTI
                     KKHLN"
     gene            complement(3637..3801)
                     /locus_tag="PC471_003717"
     CDS             complement(3637..3801)
                     /locus_tag="PC471_003717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2790795.1"
                     /translation="MFGKPKESVRFLDFNDVAEKFGEKKAVAIFDAIAGKKSKEHIIK
                     DIEKLVKNEV"
BASE COUNT         1559 a          505 c          465 g         1554 t
ORIGIN      
        1 ctatagtgtt acttatattt tatatatatg gctatttttc aacattttac aatatataac
       61 atttattttt ttaacttttg tcagtaaaat gtcagcaaaa cactttatcc acaattttta
      121 tataagtttt ccacaactta tcaccaatat tatccacata aaaaaggtag cacttatatt
      181 attagctacc ttttatttat taatctttat atttccagct ttttatccaa cttcctattt
      241 ctattatttt ttcttcagtt atttcttctc catcatgtaa ttttgaaatt aattctgata
      301 aaggattgtt atgtaaacct ctaaaaactc tttttccctc tattttgaga tattctctct
      361 cttttattaa tactgtatag tgtacatgcc tcccaattct ctatgatatt aaaaaacctt
      421 ttttggttaa tctagctaaa agtataattg tagttgtatg cttccaacca tatgtttttt
      481 ctatttcatc tgcaacatct tttgatatta cagttttata accagtatgc catatgcatt
      541 tcataatttt taattctgta tctggtaatt ttttatttag cataatatat tccttctttc
      601 aatttatgtc acttcttcaa atttatctag cttaaattat aataggagct ttttagaaca
      661 aaaactatat ttaattttta ccaggtactc tacgtgttta gtctgttttt aagtgtaaaa
      721 taatgttcat atctataaat ttatttaact ttcaaacaat cctgttgaat aataactaat
      781 tctattgtat tattttagtt gtagaataaa actaaatcgg caaaactaga gaaatttagt
      841 gacgcaaagc tatagggact aagacttata taaatatctt atgagttatg tcagccagtt
      901 gccaaagaga tagtgttctt tttgttttta tgaaagtttt ttagggggat aataatttat
      961 atgtttggaa ataaaatgga taaatgtaca catgtattga ctgcttatat tagcagttcg
     1021 tatgattatt gtaatttttt agatacacag ttagatgatt ttatattaga gtacggagaa
     1081 aatgtagtag agtcttgttt acaccaagta atggtattgg taagtaagta taattagata
     1141 gacattgatt tctagaaaac cctttttagt tttatctaca tttatattaa tatatagcaa
     1201 tgtaaaacgt ttaaatatat ttttaatatt aagaataatt aaaatgttaa ctaaaaaata
     1261 taattaagta gacatttcta ttaatctatg atacaatagt atttgtggta atacacaatt
     1321 aaatcaagta tatcttgata taggttaata ggagtatttt taatgaataa tggaatagtg
     1381 aaatggttta ataatgaaaa aggattcggt tttatatcaa tggaaggtag agatgatgtg
     1441 tttgctcatt tctcagctat acaaacttca ggatttaaat cattagaaga aggtcaacaa
     1501 gtaagctttg atatagttaa aggtgctaga ggtcctcaag cagaaaatat aactatatta
     1561 taatatatag atttttcggg aaagactctt tttagagtct ttttttattg ttaatatatg
     1621 taagataatc attggtgaag taaataaggt atattgagta atgttcttga tagtttatat
     1681 gctaatgaat ttcaaaaaaa taagcactcc tataaaaaga gtgctcttaa tgtaaatata
     1741 aaccttcatt ggtccagcct aataccccgt attctccaag ttctaactat tttttatata
     1801 cattatcact ttcatccata tctaacacaa ttatttcttc tttagaatca atttgtaaaa
     1861 tatgaggact gtgtgttgtg acaattattt gtgtttttag gagaattgct tttaatgtaa
     1921 taaaaatttt acataccaca tagttgatat aaaactataa taattacata agattgtaat
     1981 aataagactt atctttacat accacatagt tgatataaaa caatgaaatt aacatacaag
     2041 agactaagac aattaaaatc tttacatacc acatagttga tataaaaccc caaaataaac
     2101 ttagcatctc caatgtttac acatatacaa tgctcttaaa attgcagtga acgcgcaata
     2161 gtgcaattga taatacttat tatatattct caatgcctta tatttcaact gctaaacctc
     2221 aatttgtcac aaatatcgct cactgcaaaa tttctatatt tttattatat cataacatat
     2281 aattaatatt ttcaattaat ttttacattt aattatgttt aaattctata ataatgcaac
     2341 tttaatgttt ctttctggaa ctaatataaa aactatttca gaaagattag gtcatgcaga
     2401 tataaaaata actatgaata gctactctca gtttttagaa gagatggaca gagaagcatt
     2461 tgaaaatttg agcaaaatac tatttattat attttaaaga agatgactta ttcaatattt
     2521 gatgctagaa tataattaat aaaaatttaa ttataggtga ttgtattatg aatattatta
     2581 aaagcataac tttactctta acattattaa ttgtgatatt aatgacaggc tgttccccaa
     2641 gagaaagtgc tagtgaagtt gcaaaaaaat atattgataa gatgaactct ggtgactatg
     2701 aaacagcata tagtttatta agtaaagata gtaaaaaaaa aattagtata gaaaatcttg
     2761 aagaatttca aaatatttta gttagtacaa aaagaataat tagttataaa ataggtaaag
     2821 aaaaaatcat aaaaaaatat aaacatgatg gaaaagaata taaaaatgtt gttaaattag
     2881 aagaaacttt taatataaaa aaccttcttt ataaaaaaga tgattctcta aaagtaaata
     2941 gatattttgt agttgaaaat aacaaataca agctattatt gtatgaaagt ttcaaaaaag
     3001 aatttggtat taatagtatg gaccttgctc aacttaagct taatgatttt ggactaaaat
     3061 actttaaaga agttgacctt atactaaaaa aagctattgc tattaatcct actgattcaa
     3121 agctttatta tgcccaagca tgtaattata tgaattttga tggatttcct ggttattcag
     3181 atacaacata taatgcttta gattcaatta ataaatcttt aaagtattta gataaaaatg
     3241 attctgacta caaaaaaaat gcaagtaata tatataactt aaaaggagtt attcttatgt
     3301 ttcacaaaag atttgatgaa gcacaacaat atttaaataa agccttatac tttaacaaag
     3361 ataatgaaga cccaaaatat aatctaaata caataaaaaa acatctaaac tagatattta
     3421 atttttgaat ttataaaata tttttttatg tataattcag cactgaatca taatttctac
     3481 taaactaatt acaagtttaa attataaaga cttatttcta tataaatatt actctataac
     3541 aataagtttc ttatttaaat tttaaaatat tatctaaaaa tcataaaaaa gaaggtataa
     3601 agagctaccc tcaagctcaa tataccttat aaatatctaa acttcatttt ttactaattt
     3661 ttctatatct ttgataatat gttctttaga tttttttcca gctatggcat caaaaattgc
     3721 aacagccttt ttctctccaa acttttctgc tacatcatta aagtctaaaa atctaacact
     3781 ttcttttggt ttaccaaaca tataacacct ctcatacatt tttatatatt tattataact
     3841 tctatagact tatttttcac caataaacgt tagacaaatt agtcccttta tttttttaaa
     3901 ctagctttac cttcaaaatt cttaacaaaa acagtttatt ctactctata aataaaaata
     3961 gacaatttaa tttgtaggaa actgataata gaatacagta aataaaactt tgtttaatct
     4021 atctgaaaaa gttatctatt aaaactgcta tgaatttata aagaatattc tagttcataa
     4081 aaa
//
LOCUS       DAMJYR010000224         4077 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.229, whole genome shotgun sequence.
ACCESSION   DAMJYR010000224 DAMJYR010000000
VERSION     DAMJYR010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4077)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4077)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4077
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.229"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(298..516)
                     /locus_tag="PC471_003718"
     CDS             complement(298..516)
                     /locus_tag="PC471_003718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2790796.1"
                     /translation="MSVLLKTKIKSYREKNLMTQGELAKSVGVRRETIVHLENGRYNP
                     SLKLAMDIAKVFDTTVEELFEFIEENNI"
     gene            complement(517..924)
                     /locus_tag="PC471_003719"
     CDS             complement(517..924)
                     /locus_tag="PC471_003719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2790797.1"
                     /translation="MSKQQTGIKSTLISEILGISYCIGATLVNKYFFKGSSIEYLPII
                     IWVLAMGAQWVYNKKYDDLIDEASKCILSRVNDIAIKVLFFSVAIVSIFLVTLDINVS
                     NLNIGMSLLVILFIQSLLKLILFIYFDRKGIYN"
     gene            complement(1010..1498)
                     /locus_tag="PC471_003720"
     CDS             complement(1010..1498)
                     /locus_tag="PC471_003720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3841 domain-containing protein"
                     /protein_id="HDF2790798.1"
                     /translation="MKFYTIQTLEFWNNNKNNKYLSNDYSFILEDFDIPYKWMYSQMT
                     NRIYEFDDSMIWVWTKRPDLRCSGYLEKGKKGVLLEIEISEDNVLLSEFDLWHLPLMD
                     ITVELYEDEQIEKKVSWERIFDLELCSKLNEVNIEDLTLQGVTSKVPLSNVKLIKEFV
                     AK"
     gene            complement(1703..2800)
                     /locus_tag="PC471_003721"
     CDS             complement(1703..2800)
                     /locus_tag="PC471_003721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HDF2790799.1"
                     /translation="MKEKLEEKSKIIKTSKEYKRTIIEEKDREWTKKLNDILSRGDFS
                     NEKLDKIKDLIPKEILTSENVGEVVTEDGYAKPEYDEVFKSLFTLNNDYDLLANFIND
                     ILKDAPYANRNIKQFTQIKRIIKVETDPTINYIGEKRPRLDILAEDEERNHINIEMQR
                     ALEEDYLERAEYYLSRVHGRKLEEGKEYKEIGKTVGIHILNHVKYNHIEDYVNCLRLT
                     MDGHPDIYSSKTALYFIELPKIRKSSCIANRVLIWGKLIDNPSHIDIRILSKTDYVIK
                     RALDRLKELGSNEDYLLNLKRGAYIMNKSRNFKEEIFQEGIEKGIAKGKREEKFNIII
                     KLKNKGYNLSEICDIIDDLNKSEVEKVYNQN"
     gene            complement(3173..3343)
                     /locus_tag="PC471_003722"
     CDS             complement(3173..3343)
                     /locus_tag="PC471_003722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2790800.1"
                     /translation="MDSNNNTIKSTVKKGISFGSCLAMIISYTAWKSIPWAIFHGLMS
                     WIYVLYYWVKYA"
     gene            complement(3515..3958)
                     /locus_tag="PC471_003723"
     CDS             complement(3515..3958)
                     /locus_tag="PC471_003723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDF2790801.1"
                     /translation="MIEIKTIEINKKSRIDLLMLADPCEEMIDKYLNKGTMYALYDNK
                     ELTCIAVVNEISKEICELKNIATYEHFQNMGYASKMIYHLLDVYSKKYSSMVVGTSES
                     GVPFYEKFGFVYSHKIKNFFVDNYPEPIFEGELQCVDMLYLSYNF"
BASE COUNT         1380 a          668 c          363 g         1666 t
ORIGIN      
        1 atgagtcatt tattctctaa tctattagct tggtttttat ttataagtca tttattctct
       61 aatctattag cttacttctt aatctataaa tcattcattt tttaatctat tagcttggtt
      121 tttacctata aatcatttat tctctaatct ataagctttc tttttcatct ataaatcatt
      181 cattctttaa tctatgagct tggtttttca tctataagtc atttattctc taatctataa
      241 gcttggtttt ttacctataa ttaattcatt ctctaatgca taagtaatac atgaaattta
      301 aatattattt tcttcaataa attcaaacaa ctcttctaca gttgtatcaa ataccttagc
      361 aatatccatt gccagtttta aagaaggatt gtagcgacca ttttctaaat gtacaatagt
      421 ttcacgtctt actcctactg actttgcaag ttcaccttgt gtcataaggt tcttttctct
      481 ataggattta atttttgtct ttagtaatac tgacacctaa ttgtatatcc cctttctgtc
      541 aaaatatata aatagaatta acttcaacaa ggattgaata aacaaaatga ctaataaaga
      601 cattcctata tttaaatttg acacatttat gtccaaagtt acaagaaata tagatactat
      661 agctacagaa aaaaacaata cttttattgc aatatcatta actcttgata gtatacattt
      721 tgatgcctca tcaattaaat catcatactt tttattatag acccactgag ctcccatagc
      781 taatacccaa ataattatag gaagatattc aatactactt cctttaaaaa aatacttatt
      841 tactaatgta gctccaatac aataagaaat tcccaatatt tctgaaataa gtgttgattt
      901 aataccagtt tgttgtttag acatataaaa tcccccttaa aaagttatgt atttataaca
      961 cctcttgtta taaatatata acttttttga ataaatttca ataaaaatat tattttgcaa
     1021 caaattcttt tattaatttt acattagata gaggcacttt agatgtcact ccttgcaatg
     1081 tcaaatcttc tatattaact tcatttaatt ttgaacacag ttctaagtca aatattcttt
     1141 cccagcttac cttcttttca atttgttcat cttcataaag ctctacagta atatccatta
     1201 aaggtaaatg ccataaatca aattctgata ataacacatt gtcttcactt atttctattt
     1261 caagtaaaac accttttttt cctttttcta aataaccact acatcttaaa tcaggtctct
     1321 tcgtccaaac ccatatcata ctatcatcaa attcatatat tctatttgtc atttgactat
     1381 acatccattt gtatggaata tcaaagtctt ctaatataaa agaataatca tttgatagat
     1441 atttattgtt tttattgtta ttccaaaatt ctaaagtctg tatagtataa aatttcatat
     1501 ataaatcaac ccctttgttt ttctataaga aggagatggt tcaaactttt attttgaatt
     1561 atctccttta tttatacacc tctttgtacc tattatttat atatctcttt atatctacca
     1621 tatttaaata acaaagtttt atattaattt aaatttaaaa gaggaagctt tgtatttatc
     1681 tagtgccatc tcaatcatag tattagtttt gattatagac tttttcaacc tctgatttat
     1741 tcaaatcatc tataatatcg catatttcac tcaaattgta tcctttattt tttaatttta
     1801 tgatgatatt aaatttctct tctcttttcc cttttgcaat acctttttca ataccttctt
     1861 gaaaaatttc ttctttaaaa tttctacttt tattcataat atacgcacct ctctttaaat
     1921 ttaataaata gtcctcatta ctacctaatt cttttaatct atcaagtgct cttttaataa
     1981 cataatccgt tttagatagt attctaatat ctatatgaga aggattatca ataagtttac
     2041 cccatattag aactctgttt gctatacaac tggatttacg aatttttgga agttctataa
     2101 aatataatgc agttttacta gaataaatat ctggatgccc gtccattgtt aatctcaggc
     2161 aatttacata atcttctatg tgattatatt ttacatgatt tagtatatgt attccaacag
     2221 tttttccaat ttctttatat tctttacctt cttctaactt tctaccatgt acacgactaa
     2281 gataatattc agctctttcc aaataatcct cttcaagagc tctttgcatc tcaatattta
     2341 tatggttacg ttcttcatct tcagccaaaa tatcaagtct aggtcttttc tcaccaatgt
     2401 aattaattgt tgggtcggtt tcaaccttaa ttattctttt tatttgagtg aactgtttga
     2461 tattcctatt agcatatgga gcatctttga ggatatcatt tataaaattt gcaagtaaat
     2521 cgtaatcatt atttaaagta aacaatgatt taaatacctc atcatactca ggcttagcat
     2581 acccatcttc agttacaact tctccaacat tttcactagt caatatttct tttggaatta
     2641 aatccttgat tttatctaat ttttcattag aaaaatcacc tctactaagt atgtcattta
     2701 attttttggt ccattctctg tccttttctt caattatagt tcgtttatac tcttttgatg
     2761 ttttaataat cttagatttt tcttctaatt tttctttcat atcaacacct cttttttatt
     2821 attacccatt tcttcatacc aaagtctgaa cttctaagta aaatttgcat tattgaaata
     2881 agaaccttat aaacttatat atcattacta aagttaagac tttaaagaga agcacttaga
     2941 ttacaataga ttttagatag ctttcttttt aaaactattg ttgataaatg aatgaatata
     3001 agggtatgta ttaaaataga gatactcatc tttttcattg tttaaataat gaaaatatat
     3061 tttatttaat acaaaaaaga gtgccttata aactaaaagt tcacttcgag acactccctt
     3121 acattttgtt tcttaaactt tacaatatta catacttatt tattaattta atctatgcat
     3181 acttaaccca ataataaagt acatatatcc aactcattaa gccatgaaaa atagcccatg
     3241 gaatagattt ccatgcagta taagaaataa tcattgctaa acaagaacca aaagaaatac
     3301 cctttttaac agttgatttt atagtattat tatttgaatc cataattaat cctccaaaat
     3361 tcaaaataaa caagttatta tatatcttat ttgaatataa tacaatataa cagcttctaa
     3421 ataaattata ctcttattat acataattta taaattataa ttccgataat tatatgaatt
     3481 aattaattat attaacctaa aaatattatc ctatctaaaa gttatatgat agatacaaca
     3541 tgtcaacaca ttgtaattct ccttcaaaga ttggttctgg ataattatct acaaaaaagt
     3601 ttttaatttt atgtgaataa acaaatccaa acttctcata aaaaggaact ccgctttcag
     3661 atgtaccaac aaccattgat gaatattttt tactataaac atctaaaaga tgataaatca
     3721 tctttgaggc ataacccata ttttgaaaat gctcatatgt agcaatgttt tttaattcac
     3781 atatttcttt ggaaatttca tttacaactg ctatacaagt aagttcttta ttatcataca
     3841 atgcgtacat agttccttta ttgagatatt tatcaatcat ttcttcacat gggtcggcta
     3901 acattaaaag gtcaattcga ctttttttat ttatttctat agtttttatt tctatcataa
     3961 gttctccacc ctcactttat tcaaacctta atttaacaaa cttcttacag tacttattta
     4021 attcattttg atatatattt ttacttttta aaatatagtg ataattccct agaatat
//
LOCUS       DAMJYR010000225         3977 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.230, whole genome shotgun sequence.
ACCESSION   DAMJYR010000225 DAMJYR010000000
VERSION     DAMJYR010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3977)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3977)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3977
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.230"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(224..604)
                     /locus_tag="PC471_003724"
     CDS             complement(224..604)
                     /locus_tag="PC471_003724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HDF2790802.1"
                     /translation="MCLKKLSKLELVIMKFIWNLDIKTNSYEIIDYMKEEHNLPEKVA
                     LKTLSKLSKKRFLYVQETGKCMYYTVAIKEKAYLEFISRNVQNLLKNNFIRNLLVSFH
                     EEELTEEKIKSLENWVVNWEEAYV"
     gene            complement(805..1998)
                     /locus_tag="PC471_003725"
     CDS             complement(805..1998)
                     /locus_tag="PC471_003725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HDF2790803.1"
                     /translation="MLTSLALIFLLGMASGGIFKRIKLPNLLGMLLTGIILGPYVLNL
                     IDNSILDISSDLRKIALIIILTRAGLSLDINDLKKVGRPAVLMCFIPATFEIIGMIVL
                     APKLLGVSILEAAVMGAVVGAVSPAIIVPKMLKLMEEGYGTEKSIPQMLLAGTSIDDI
                     FVIVMFTVFTGLAQGNSISAISFLQIPVSIILGVIVGAVIGICLAVFFKKVHMRDSAK
                     GVLLLSISFLMISLETALEGIVPFSGLLAVMSIGIFLQIKYRVVARRLSIKYSKLWVG
                     AEILLFVLVGATVDISYAFKAGIGAVILIFGVLLFRMVGVFFCLIKTNLTIKERLFCM
                     IGYIPKATVQAAIGGVPLAMGMASGQLILTLAVLAILITAPLGAFGIDVTYKKLLTSV
                     KGESK"
     gene            complement(2061..3317)
                     /locus_tag="PC471_003726"
     CDS             complement(2061..3317)
                     /locus_tag="PC471_003726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HDF2790804.1"
                     /translation="MIEKFKTLIWIKMIIRIKSVYKTYGGLFFLGLIGIPVGAIIGLI
                     DTIFGTVLLKVTDIRETYPMYLIPFLAVVGVVIAYCYFKFGGKSSKGMNLIFEVGHGE
                     EEIIPLRLVPFIISGTWLTHLFGGSAGREGVAVQIGATFSHWVGKRLPIKNACSIFLV
                     TGMAAGFAGLFETPIAAILFAMEVLVAGSLEYQSLFPAFTASFTASSVSKALGLEKFS
                     FALSSKVVFDLSIFWKLIVLGIIFGMVGGAFAWCLKLSKRKIGNRLKNPMIRIAIIGV
                     CLSVLFLLFYKGRYSGLGTNLIQNSFYGGEIYSFDWLLKFILTILTLSAGFQGGEVTP
                     LFSIGASLGVLLAGFFNLPIELVAALGYASVFGSATNTFFAPVFIGAEVFGYSYLPYF
                     FVVCAISYIFNMDKSIYSLQKISTKQ"
BASE COUNT         1441 a          733 c          415 g         1388 t
ORIGIN      
        1 agaatagaaa caagttctag attactatag agatgacata tcaaaatatt tttaaaaagt
       61 aatttaagta ttagaagtaa ttgaagttag taaatataaa tgcgtttact tttaagtatt
      121 gcttaaggga ataaaaaata aaaataaact ctgcaaccat ctaaaaatag ttgcagagct
      181 tacaaaacca aaattatcta aattgtaata aaaataataa catttataca taagcttctt
      241 cccaatttac tacccagttt tctaacgact ttatcttttc ctctgttagc tcctcttcgt
      301 gaaatgatac taataaattc cttataaaat tattcttcaa aagattttgc acatttcttg
      361 atataaactc taaatatgcc ttttctttta ttgcaactgt ataatacata cacttgcctg
      421 tttcttgaac atataagaat cttttctttg atagttttga taaagttttt aaagctactt
      481 tttctggtaa attatgttct tctttcatat aatcaataat ctcatatgaa ttggttttta
      541 tatctaaatt ccaaataaat ttcataataa ctaactctaa ttttgataat ttttttaaac
      601 acatagttat cactccgctt ctttatctat ataattaaat attacaataa taatttcaca
      661 aaatcaattc taataatcat aacatacctt aaaatctgtt cttaacttct accttcttaa
      721 tctcccaaat gattttttta tattgtattc aatagtataa tccttttcca aaatataact
      781 taccagttaa aataatcttt atttttactt actctcgcct tttacagaag ttaataattt
      841 tttataagtt acatcaattc caaaagctcc aagaggtgca gttataagta tagctagaac
      901 tgctaacgtc aaaataagtt gtccagatgc catccccatt gcaagtggta cacctcctat
      961 tgctgcctgt actgtagcct ttggaatata gccaatcata cagaacaacc tttcctttat
     1021 tgtcaaattt gtcttaataa ggcagaagaa tactcctacc atacggaata aaagaactcc
     1081 aaatattaaa attacagctc caattcctgc tttaaatgca taacttatat ctactgttgc
     1141 ccctaccaaa acaaacaaga gaatttcagc gccaacccat agttttgaat actttatgga
     1201 caatcttctt gcaactacac gatattttat ctgaaggaaa atcccaatac tcataactgc
     1261 taataatcca gaaaatggca caataccctc taaagcagtc tctaaactta tcattaaaaa
     1321 tgagatactt aaaagcaaca cccctttagc agaatctctc atatgaacct tcttaaaaaa
     1381 tactgctaaa cagattccaa tcactgctcc aactattaca cctaatataa ttgatactgg
     1441 aatttgtaaa aaactaatag ctgatatact atttccttgt gccaatcctg tgaatactgt
     1501 aaacatcaca attacgaaaa tatcgtctat agaagttcct gctaaaagca tctgtggaat
     1561 acttttttct gtaccataac cttcttccat taatttaagc attttaggta caataattgc
     1621 gggagaaact gctcctacta ctgcacccat gactgctgct tccaatatag aaacgcctag
     1681 taattttggt gctaaaacta tcattccgat aatttcaaaa gtagctggaa taaagcacat
     1741 aagtacagca ggtcttccca ctttcttcaa atcgtttata tctaaagata atccagccct
     1801 tgttaatata ataatcaatg caatctttct aaggtcagaa gaaatatcta gtattgaatt
     1861 atcaatcaaa tttaaaacat atggacctaa tataattcct gtaagtaaca ttcccaataa
     1921 gttaggtaac tttatcctct taaaaatacc tcccgaggcc attcctaata aaaaaatcaa
     1981 agctaaactt gtcaacatat tcctacccta tcctttctct aatattcttt ctcttctaat
     2041 catacattat gttgttttat ttattgtttt gttgaaattt tttgtagtga ataaatagat
     2101 ttatccatat taaatatata tgaaatagcg catactacaa agaaataagg taaatagctg
     2161 tatccaaaaa cttctgcccc tataaataca ggtgcaaaaa aagtatttgt agcactacca
     2221 aagacactag catagccaag cgctgcaact aattcaattg gcaaattaaa aaatcctgct
     2281 aacaatacac caagactagc tccaatggaa aacaaaggtg taacctctcc tccttgaaaa
     2341 cctgctgaca aagtcagtat tgtaagtata aactttaaca gccaatcaaa tgaataaatt
     2401 tctccaccat agaaactatt ttgaattaaa tttgtcccta aacctgaata tcttccttta
     2461 taaaataaaa ggaataatac actaagacat actccaataa tagcaattct tatcatagga
     2521 ttttttaatc tattacctat ttttcttttt gacaatttca gacaccatgc aaatgctcca
     2581 ccaaccattc caaaaataat tcctaaaaca ataagcttcc aaaatattga caagtcaaat
     2641 acaactttag aagaaagtgc aaaagaaaat ttctctaacc ctaaagcttt tgatactgaa
     2701 cttgctgtaa aagaagcagt aaacgcagga aaaagcgatt gatactccag tgaaccagct
     2761 accaaaacct ccatagcaaa tagtattgct gctattggtg tctcaaataa tcctgcaaaa
     2821 ccagctgcca taccagttac tagaaaaata ctacaagcat tttttatagg caacctttta
     2881 cctacccaat gtgaaaaagt tgcaccaatc tgaacagcaa ctccttctcg tcctgcacta
     2941 ccaccaaata aatgggttaa ccatgtacct gatattataa atggaaccaa tcttaaagga
     3001 ataatttctt cttctccatg ccctacctca aatattagat tcattccttt actacttttt
     3061 cctccaaatt taaaatagca ataagcaata acaactccta ctactgctaa aaatggtatc
     3121 agatacattg gatatgtttc tcgaatatct gtaactttta gaagaaccgt tccaaatatg
     3181 gtgtctataa gacctataat tgcaccaact ggtataccta tgagtcctaa aaaaaatagt
     3241 ccaccatatg ttttataaac gcttttaatt cttattatca ttttaatcca tattaaagtt
     3301 ttaaattttt ctatcatttt aattcctttc tatttgcatc ataagatttt ttgaaagttt
     3361 tattacatca actgtagtca atgtaatgac tatcataatt gttttttaaa ttgataggat
     3421 ataggtacac tcttaaccca ttcttaataa aataattaac atataatcta ctattttttc
     3481 tattaattaa atatttcttc aaatttaaaa aataaaaatt tagtaagtac ttcacctcca
     3541 ttttattgat ttaaacaaca aaaaaagctg gtggcatcta tgatatcata gaagtcatca
     3601 gctcattggc agtttaggat attatcatcc aagggagaac ctcattccca taattattat
     3661 ataatttttt atttttcata tgtctatttt atgtaaattg tatttttaat gttataaata
     3721 atataaccta tcaattaaaa tagtaaagcc tctaatatta tgagtgttca atatttcata
     3781 ttatttttat gatatataat ttaaatgatt ttctttactt tgcattatga tacataactt
     3841 aaatattttt cctcactcca cattatgata tattatttaa atatttttct tcactctgct
     3901 tatattatga tacatgattt aaatattttc ttcactctgc ttatattatg atacatgatt
     3961 taaatatttt cttcact
//
LOCUS       DAMJYR010000226         3928 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.231, whole genome shotgun sequence.
ACCESSION   DAMJYR010000226 DAMJYR010000000
VERSION     DAMJYR010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3928)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3928)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3928
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.231"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            <1..606
                     /locus_tag="PC471_003727"
     CDS             <1..606
                     /locus_tag="PC471_003727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2953 domain-containing protein"
                     /protein_id="HDF2790805.1"
                     /translation="FKYLIIAVIFIISVTMLILISHINILIIADIKNKDICLKLNIKY
                     MFNLININRQLYPAENSKNNDKKEGMKNNIDSSILLADDLLSIYRLLKKIKIHELYSN
                     INFGTGNIGLTSSVYVLINTLYGNLFNMIDAEKMYLNVNPDFTKDYVLGNIRIHIRPR
                     IKALFNIIIMINKIMNKNKGNKEGDSNESNRFDTESYGNNS"
     gene            560..988
                     /gene="ytfJ"
                     /locus_tag="PC471_003728"
     CDS             560..988
                     /gene="ytfJ"
                     /locus_tag="PC471_003728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GerW family sporulation protein"
                     /protein_id="HDF2790806.1"
                     /translation="MRATGSIQSLMETTLETIKGSIDANTIIGDPIKTDTTVVVPISK
                     VTIGFGIGGGEYSKGYDDKDREIELKNEKSDTNFAGGSAGAISVQPVAFVVVESGETR
                     IMSLDSNINLVDNILSITPRVLEKIQNISQSNKNQDKNNM"
     gene            1236..1676
                     /locus_tag="PC471_003729"
     CDS             1236..1676
                     /locus_tag="PC471_003729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDF2790807.1"
                     /translation="MSDKELSILLLESMNNFHNLIKIINNERYKKDKVLTERQFFALV
                     KIRKHDKIELKNLSRDLHVSTSSLCILLNKLVEQEYVYREEDSRDRRNTFYGITKNGE
                     KILDNEILKFVSIISDKMDCLDINNKDKLFTSLEESKNIIEQLF"
     gene            1692..3143
                     /locus_tag="PC471_003730"
     CDS             1692..3143
                     /locus_tag="PC471_003730"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HDF2790808.1"
                     /translation="MNIKYSKEILKSLENAITVVALFEGEDVSTTFDKINMISRNFNS
                     KENSIDTVTLVENNNLVNVCLVGFGKRELLNREKIRIIGGNLCKKLKNILKNEAYKSS
                     DVNVLNLNLNSDEIGCFVEGILLGNYKFDKYKTKSKDFERKEVDTITIFTEKEVECQI
                     EKAKILANSTIIARNLVNEPSNVIYPKTLAVETVKLGAEFGFNVDVYEEEKIKALGMD
                     AFFAVSRGSINKPRFIVMRYFGDKESTDILGLVGKGLTYDTGGYSLKSNASMLDMKTD
                     MAGAASVIGAMCAISQSKLKKNVIAVVAACENALSGGSYKPGDIISSMAKKTIEVLNT
                     DAEGRLTLADAIYYIINNEKVTKVVDVATLTGAALILLGNVATPIVTNNDDFYCELEK
                     AATLSGERVWKMPIYDEFKDMIKGEEADLKNTGGKNAGCITAGAFIGEFVGNTPWIHM
                     DIAGTSTSSKSIGYKAKGATGEPVRTLYYLAEI"
     gene            complement(3234..3902)
                     /locus_tag="PC471_003731"
     CDS             complement(3234..3902)
                     /locus_tag="PC471_003731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YwaF family protein"
                     /protein_id="HDF2790809.1"
                     /translation="MFKIFLFSSEQFVSLFIFGLFLYYCPKLTKNILPYSYTVEKIIC
                     TLLVIIMALEQLLLISSGNYSTLNSLPIGINYICIYLCIAILIFKQYHLFNIFFSWSL
                     VCSVGELIFSKNLGYEFPSLIYFIFIFSKCLIIYADIYMVDVRKFRVNRYALRDNLAI
                     CFIYFSFIFLLNTFTNSRYYYGFLSHSTTAIFTFIFVTSIMYIPALLFNRDTFILEKK
                     KKSK"
BASE COUNT         1642 a          357 c          671 g         1258 t
ORIGIN      
        1 tttaagtatt taataatagc tgtaattttt attataagtg taactatgtt gattttaata
       61 tctcatataa acatcttaat aatagctgat ataaaaaaca aagatatttg tttaaagctt
      121 aatataaaat acatgtttaa tttaataaat ataaatagac aactttatcc agctgaaaat
      181 agtaaaaata atgataaaaa agaggggatg aagaataata tagattcaag tatattatta
      241 gctgatgacc ttttgagcat ttatagatta ttaaaaaaga taaaaataca tgaactttat
      301 tcaaatatta attttggaac tggaaatatt ggcttaacat cttctgtata tgtattgata
      361 aatactttgt atggaaattt atttaatatg attgatgcag aaaaaatgta tttaaatgta
      421 aatccagatt tcacaaaaga ttatgtttta ggaaatataa ggatacatat aaggccaaga
      481 ataaaagctt tatttaatat tataataatg attaataaaa ttatgaataa aaataaagga
      541 aataaggaag gtgatagtaa tgagagcaac aggttcgata cagagtctta tggaaacaac
      601 tcttgaaaca attaaaggtt ctatagatgc aaatactatt ataggagacc ctataaaaac
      661 agatactaca gtggttgtac ctatatcaaa ggttacaatt ggctttggta taggaggagg
      721 agaatactca aaaggatatg atgataaaga tagggaaatt gaattaaaaa atgaaaaatc
      781 agatacgaat tttgctggag gtagcgcagg ggctatatct gtacaaccag tagcatttgt
      841 agtagttgaa agtggagaga ctagaataat gagtttagat agtaatatca atctagtgga
      901 taatatatta tctataactc ctagagtatt ggagaaaatt caaaatatat cacaaagtaa
      961 taagaatcaa gataaaaaca atatgtaaga ataaaatatt tttaaatatg tatatagttt
     1021 taagtaaagt agtggggtta atataaatcc cactattttg ttgtgtactt taaacattta
     1081 tatctaataa gattttagtt taaaacaatt taaatagata ataaagttga tatatagtat
     1141 aattataaaa agaaaagaag tattatatta tagatatata aacgcatttg agatataaga
     1201 gggagttata aatcattagt tttgaggtga atgttatgag cgataaagaa ttatctattt
     1261 tgttgttaga aagtatgaat aattttcata atttaataaa gattattaat aacgaaagat
     1321 ataaaaaaga taaggttctt acagaaagac agttctttgc cttagtaaaa ataagaaaac
     1381 atgataaaat agagcttaaa aatctaagta gagatttgca tgtatctact tctagtctgt
     1441 gtatactact taataaattg gttgagcaag agtatgtata tagggaagaa gatagtagag
     1501 atagacgaaa tactttttat ggaataacta aaaatggaga aaagatattg gataatgaaa
     1561 tactaaagtt tgtatctata ataagtgata aaatggattg cctagatata aataataaag
     1621 ataaattatt tacttctttg gaagagtcta aaaatataat agaacaatta ttttgaaaag
     1681 gagaatttat tatgaatata aagtattcta aagaaatatt aaaaagttta gaaaatgcta
     1741 taacagtagt agctctattt gagggagaag atgtttctac gacatttgat aaaataaata
     1801 tgataagcag aaattttaat tctaaagaaa atagtataga tactgtaaca ttggttgaaa
     1861 ataacaattt ggtaaatgtt tgtcttgttg gttttggtaa gagagagtta ttgaatagag
     1921 aaaaaattag aatcataggt gggaatttgt gtaaaaaatt aaaaaatata ttgaaaaatg
     1981 aagcatataa gagtagtgat gttaatgtac tgaatcttaa tttaaatagt gatgaaatag
     2041 gttgttttgt agaagggata ttacttggaa attataagtt tgataaatat aagacaaaaa
     2101 gtaaggattt tgaaagaaaa gaagttgata ctataactat atttactgaa aaagaagttg
     2161 agtgtcaaat agaaaaggct aaaatattag ctaattcaac tataatagct agaaatctag
     2221 ttaatgaacc atcaaacgta atatatccaa aaacattagc agtagaaaca gttaaacttg
     2281 gtgcagagtt tgggtttaat gttgatgttt atgaagaaga aaaaataaag gcattgggaa
     2341 tggatgcttt tttcgctgtt tcaagaggtt caattaataa accaagattc atagtaatga
     2401 gatactttgg agataaagaa agtacagata tattaggtct agtaggtaaa ggattgactt
     2461 atgatactgg tggatattct ttaaaatcaa atgcttctat gctagatatg aagacagata
     2521 tggctggtgc tgcgagtgtt ataggtgcta tgtgtgctat atctcaaagt aaattaaaga
     2581 aaaatgttat agcagtagtg gcagcatgtg agaatgcttt atcaggaggt tcatacaaac
     2641 ctggagatat aattagttct atggcaaaga aaactattga agttttaaat acggatgcag
     2701 aaggtagact tacattagct gatgcaatat actacattat aaacaatgaa aaagtaacaa
     2761 aggtggttga tgtagctaca ttaactggtg ctgctttaat attgttaggt aatgtcgcaa
     2821 ctcctatagt aacaaataat gatgattttt attgtgaatt agaaaaagct gccactttat
     2881 caggggaaag ggtatggaag atgccaattt atgatgaatt taaagatatg ataaaaggag
     2941 aagaagcaga ccttaaaaac actggtggta aaaatgctgg atgtataaca gcaggagctt
     3001 ttattggtga atttgttggg aatactcctt ggatacatat ggatatagcg gggacaagta
     3061 catcatcaaa atcaattgga tacaaagcaa agggggctac aggagaacct gttagaacat
     3121 tgtactattt ggcagaaatc taaaaaacaa ttataaaata taatttataa agccacgtgg
     3181 atatttttgt aatttaagat tatctatgtg gctttatttt ttatttaaat aaattattta
     3241 ctctttttct ttttttcaag tataaaagta tctctattaa ataaaagagc aggtatgtac
     3301 ataatactag taacaaaaat aaacgtaaaa atagcagttg tgctatgact taaaaaacca
     3361 taatagtacc tagagttggt aaatgtgttt agtaaaaata tgaatgaaaa ataaataaaa
     3421 catatagcta agttatctct aagtgcatat ctgttaactc taaattttct aacatcaacc
     3481 atatatatat ctgcataaat tatcaagcac ttagagaaga taaagataaa atatattaag
     3541 ctaggaaatt catagcccaa gtttttagaa aaaatgagtt ctccaactga acaaactaaa
     3601 ctccaagaaa aaaatatatt aaaaagatga tactgcttaa aaattagtat ggctatacac
     3661 agatatatac aaatataatt gattccaatt ggtaaagagt ttagagtact ataattacct
     3721 gaagaaataa gtaataattg ctctagagcc ataattatta caagtaatgt acaaattatc
     3781 ttctcaactg tatagctata aggtaaaata tttttggtta gttttggaca atagtataaa
     3841 aataatccaa atataaagag acttacaaat tgttcactag aaaaaagaaa aattttaaac
     3901 atataacatt ctctcctata ataaattt
//
LOCUS       DAMJYR010000227         3873 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.232, whole genome shotgun sequence.
ACCESSION   DAMJYR010000227 DAMJYR010000000
VERSION     DAMJYR010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3873)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3873)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3873
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.232"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            86..760
                     /gene="fsa"
                     /locus_tag="PC471_003732"
     CDS             86..760
                     /gene="fsa"
                     /locus_tag="PC471_003732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HDF2790810.1"
                     /translation="MQLILDTGNVEEIKELCTCLPIDGVTTNPSIVSKEKKNFKQLIN
                     EIGEIIGEDMPIHAQVLSTKYEEILEEALYISSLRKNIYVKIPVTQDGLRAIKDLHRK
                     GVKITATAIFTAHQGFLAAKAGANYIAPYVNRLDNISGDGVAMVSELIKIIDTYKMDT
                     KVLAASFKNAQQVIELMQHGVHSVTVPYDICKSMMNHPLTDWSVGKFIEDWENTFGKG
                     SKTNNI"
     gene            complement(882..2276)
                     /locus_tag="PC471_003733"
     CDS             complement(882..2276)
                     /locus_tag="PC471_003733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HDF2790811.1"
                     /translation="MGIILKPKQFYMTVIATIILYFSLNYSFNIKFSICLSFLTILIW
                     AVDSVEKTFVALFFVILAFIFNIAPIKVILQFLFTENFYIIILAYIITNAVTKTGVAK
                     IISEKLILNTVNTPKKMILLSYILGILLIFFIPQPFPRVILVSAFYKEFLKEQQITED
                     SKSILLFSIFTASTFTSMFFVNGDTLLNYVVLELGNCNINWGQWAFYMSVPTIITCFI
                     TYFLFIFVFKKELHSIKFISNENLEGNKLHFSYSNLKKEFNLLNKEQKHVFLCLGIMF
                     FMFLTQFIHNINTLIIMSICVLLLLLRKIVGFSTLKEINWKVLIFFIAAFSIGGVLKY
                     SGVVDIMGNYLIKFIPNSSEIISILFLITLTIILNICLGSAVTTSSVVIPLLGSLHIL
                     KENSVTLCLFVYIIVSIQYILPFHHATIMVGHGENLYNSKVIFKYGLTLIPLTYFIII
                     CITFPWWKFIGLEI"
     gene            complement(2354..3700)
                     /locus_tag="PC471_003734"
     CDS             complement(2354..3700)
                     /locus_tag="PC471_003734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HDF2790812.1"
                     /translation="MKDLTTGHEGKSIFFFAMPMLIGSLFQQLYNTADSIIVGRFIGK
                     EAMAAVSGANPIMFLLVAALMGVSLGFSILVSQFYGSGDLKKVKATIDTTYILLFIGS
                     ILISILGIVFGGPMLKLMNTPESVFAQSKLYLTIIFSGILFSAGYNSVSAILRGLGDS
                     VTPLYFLIIATILNIVLDLTFIVVLRMGVEGVALATIMAQAVSFIISIIYLNKKHEVL
                     KFKIKGIVYDNKIFKDGLRLGLPSGVQQMLFSIGNMALQFLVNSYGTSAMAAFGAGLR
                     IENFISLPIMNLGSAVSTFVAQNIGAGENERVKKGIRESIKMTLVLAVTVIALILLFR
                     ENLIALFNTDKDVIKIGSSYLFIIGPFFLFIGTSFVLSSAMKGAGDSMFALISSIVSL
                     WLGRLPASYMLSKFFGTDGIWMGIPFGWTLGLIVTVIYYKKGHWKTKAIVNHRINE"
BASE COUNT         1535 a          535 c          533 g         1270 t
ORIGIN      
        1 aataaaaaac aataagcaaa atgttgaaaa taataccaaa tcaatatata ataagaataa
       61 agaaagatta aagggggata ttttaatgca actaatctta gatacaggta atgtggagga
      121 gattaaggag ttatgtactt gtttaccaat agatggtgta actacaaatc caagtattgt
      181 ttcaaaagaa aaaaagaatt ttaagcaact tataaatgaa attggtgaga ttataggtga
      241 ggatatgcct atacatgctc aagtgttaag tacaaagtat gaagaaatat tagaagaagc
      301 attatatata agtagtttga gaaaaaatat atatgtaaaa atacctgtaa cacaagatgg
      361 tttaagagct ataaaagacc ttcacagaaa aggtgtaaaa ataactgcta ctgcaatatt
      421 cactgctcat caaggttttt tagctgcaaa ggctggagca aattatattg caccttatgt
      481 aaataggctt gataatatat ctggtgatgg agtagctatg gtttcagaac ttataaaaat
      541 aatagatact tataaaatgg atacaaaagt acttgcagcc agttttaaaa atgcacaaca
      601 agttatagaa ttaatgcaac atggggtgca tagtgttaca gtaccatatg atatttgtaa
      661 atctatgatg aatcatccat taactgattg gagtgtaggt aagtttatag aggattggga
      721 gaatactttt ggaaaaggaa gtaaaacaaa taatatttag tattatggta aaagagtagt
      781 tttgtccaat ttcacaagaa cttatataaa aagatgaaaa tatgaatttt ctttttaata
      841 atttcgttat tatcttaagt taattcatat tttcatcttt tttagatttc tagtcctata
      901 aatttccacc aagggaatgt tatacatatt attataaagt aggttaaagg aatcaaagtt
      961 aaaccgtatt tgaaaataac cttgctatta tacagatttt caccatgacc aaccattatt
     1021 gtagcatgat gaaatggtaa tatgtactgt atgcttacta taatgtagac aaatagacat
     1081 aaagtaacac tattttcctt tagtatatgt aaacttccaa gtaacggtat tactacagat
     1141 gatgtagtga cagcacttcc aagacatata ttcaagatta ttgtgagagt tatcaaaaac
     1201 aatattgaaa taatttctga agagtttggt ataaacttta tcaaataatt tcccattata
     1261 tctacaactc cagagtactt taacactcct ccaattgaaa atgctgctat aaaaaaaata
     1321 aggaccttcc aatttatttc ttttaaagta ctaaatccaa ctatttttct tagtaataaa
     1381 agaagtacac aaattgacat tataattaat gtgtttatgt tatgtatgaa ctgagttaaa
     1441 aacataaaaa acattattcc taggcataaa aatacgtgtt tttgctcttt gtttagcaaa
     1501 ttaaattctt tttttaaatt agaatatgaa aaatgaagtt tattaccctc aagattttca
     1561 ttagaaataa attttattga atgcaattct tttttaaaga caaagataaa taaaaaataa
     1621 gttataaaac aagtaattat agttggaaca gacatataaa aagcccattg accccaattt
     1681 atattacaat ttccaagttc tagtacaaca taatttagaa gggtgtctcc atttacaaaa
     1741 aacattgatg taaaggtaga tgctgtaaat atactaaata aaagtataga ttttgaatct
     1801 tcagttatct gttgttcttt taaaaactcc ttgtaaaaag cagatacaag aataaccctt
     1861 ggaaatggtt gtgggataaa aaatattaaa agtataccta aaatatacga taaaagaatc
     1921 atttttttag gggtatttac agtatttaaa attaacttct cagaaataat ttttgcgaca
     1981 cctgtttttg taactgcatt agtaattatg tatgcaagaa ttattatgta aaaattttct
     2041 gtaaataaaa actgtaaaat aacctttatt ggtgcaatgt taaatataaa cgctagaatt
     2101 acaaaaaaca atgcaacaaa agttttttca actgagtcta cagcccatat caagatagtt
     2161 aaaaaagata agcatataga aaattttatg ttgaaactgt aattaagaga gaagtagaga
     2221 attatggtag ctataacagt catataaaat tgttttggct taagaataat acccattttt
     2281 atattcacct ccataattta ttatatcata atggaggtga atacaatgaa tgtgaataca
     2341 ttttataaaa agattattca ttgattctat gatttacaat agctttagtt ttccaatgtc
     2401 ctttcttata gtagataact gtaacaataa gacctaaagt ccaaccaaaa ggaataccca
     2461 tccaaattcc gtctgttcca aaaaatttag aaagcatata agaagctgga agtcttccaa
     2521 gccataaaga tactattgag cttataagtg caaacatgga atcaccagca cctttcattg
     2581 cacttgaaag tacaaaagaa gttccaataa ataagaagaa tggtcctatt ataaataagt
     2641 aagaagagcc aattttaatg acatctttat cagtattgaa tagagcaatt agattttccc
     2701 taaatagcaa aattaaagca ataactgtaa ctgctaaaac taaggtcatt tttatagatt
     2761 ctcgaatgcc ttttttaact ctttcatttt ctccagcacc tatgttttga gcaacaaaag
     2821 tagaaacagc agaaccaaga ttcataatag gtaagcttat aaaattttct atcctaagac
     2881 cagcaccaaa agctgccata gcagatgtac cataactatt aactaaaaat tgaagagcca
     2941 tatttccaat tgaaaacaac atttgttgaa ctccacttgg aagtcctaat ctaagtccat
     3001 ctttaaatat tttgttatca tatacaattc ctttgatttt aaatttaagc acttcatgtt
     3061 ttttatttaa ataaattata cttattataa atgatactgc ctgagccatt atagtggcaa
     3121 gtgcaacgcc ctcaacaccc attcttagca ctacaataaa agttaagtct aaaactatat
     3181 ttagaattgt ggctattatc agaaaataaa gtggagttac tgaatctcca agtcctctaa
     3241 gtattgcact tactgaatta tatccagcag aaaataatat tccactaaat attatagtaa
     3301 gatacaattt tgattgagca aaaacggatt ctggtgtatt cataagctta agcattgggc
     3361 caccaaatac tattccaaga atacttatta atatagaacc aataaataaa agtatatatg
     3421 tagtatcaat agtagctttt acctttttta aatctcctga gccataaaat tgtgacacta
     3481 aaatagaaaa tccaaggcta acacccataa gcgctgccac caataaaaac attatagggt
     3541 tagctccaga gactgccgcc attgcttctt ttccgataaa tctaccaact attatactgt
     3601 cagctgtatt gtatagttgt tgaaataaac taccaataag cataggcatg gcaaaaaaga
     3661 atatggactt tccctcatga cctgtagtta aatctttcac cttattcctc caaataaaaa
     3721 aattattaat ttattatagt ataatcttaa cataaacttt atatccaaac tataaataaa
     3781 ttgtatcaaa atatcatata tttttttaaa atgtaaagaa tataattata atttgaattt
     3841 aataaatcaa tatttaatat aatcgaatat taa
//
LOCUS       DAMJYR010000228         3788 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.233, whole genome shotgun sequence.
ACCESSION   DAMJYR010000228 DAMJYR010000000
VERSION     DAMJYR010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3788)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3788)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3788
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.233"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(<1..368)
                     /locus_tag="PC471_003735"
     CDS             complement(<1..368)
                     /locus_tag="PC471_003735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HDF2790813.1"
                     /translation="MKQQEVVNKLRRGELMLMEYLWRKNSILSKKEIIEAMKEKYKWR
                     KSTTEILLKRLVKMKTLKKKRIGFQFSYEVLVTKKEYLNAIKEDRKVTKYDSFFIRMF
                     TTMHKKEKMTEEQIKKYIEN"
     regulatory      577..674
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            729..905
                     /locus_tag="PC471_003736"
     CDS             729..905
                     /locus_tag="PC471_003736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2790814.1"
                     /translation="MFKNKMDKCTHMLTAYISSSYDYCNFLDTQLDDFILEYGENVVE
                     SCLHQVMVLVSKYN"
     gene            complement(1124..1300)
                     /locus_tag="PC471_003737"
     CDS             complement(1124..1300)
                     /locus_tag="PC471_003737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2790815.1"
                     /translation="MKTLMFILVIAYYITIVIKSITTIVKSITRLLDSISRLKESLHK
                     LRNRPDGNQDDFNE"
     gene            1554..1742
                     /locus_tag="PC471_003738"
     CDS             1554..1742
                     /locus_tag="PC471_003738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2790816.1"
                     /translation="MTKTILCDYCNKGINKDDNKYITFHKKSHMKTNICINCALNLID
                     KDKLNENIINNQHDYSKK"
     gene            complement(2152..2736)
                     /locus_tag="PC471_003739"
     CDS             complement(2152..2736)
                     /locus_tag="PC471_003739"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2790817.1"
                     /translation="MKGEYNKEKEKVIKFNSNKDKSKINKYTDYYNTLNSTLMEKNKC
                     IGDNWFLATTSEEVGNMNEEEKRILETEKRILETEKRVHQNNKEVKESIDKQFNDIKG
                     IFKEYKNDLDKDRIELKNDLARERIELKSDLKEAINEHKKVTEKDVSEIKNSIKGIED
                     RIDSTNKWIIALCITTIIGIATMAITIGISIWPK"
     gene            complement(2739..3230)
                     /locus_tag="PC471_003740"
     CDS             complement(2739..3230)
                     /locus_tag="PC471_003740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016056344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2790818.1"
                     /translation="MAYENGTNLVAYFPNCIKKYEKDNKIFEKTILLLNLYMQMPTWP
                     HCIHEFKAKANEILGEDPEFIGEYGIKLKDFYNEGENVNISQNFKNDLYTFSNSTYDI
                     LDNSYIQRINPIGLYSISSLARENDFILKLFRNDKKSLELELSKSEIQSIIEHLESMI
                     EEE"
     gene            complement(3480..>3788)
                     /locus_tag="PC471_003741"
     CDS             complement(3480..>3788)
                     /locus_tag="PC471_003741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HDF2790819.1"
                     /translation="IVEGVLNKNINNEGVKQMYKHTIVYDGEVDKISATVVGWGYNDG
                     KILICDIKDYVPGQTQNLYVVGGVACEKIGSMTKEKFTMIKGNDRFDTLYKALEFINK
                     "
BASE COUNT         1240 a          544 c          390 g         1614 t
ORIGIN      
        1 atattttcta tatatttttt tatttgttct tctgtcatct tttctttttt atgcatagtg
       61 gtaaacattc gtataaaaaa actatcatat ttagttacct ttctatcttc ttttattgca
      121 tttaaatatt cttttttagt tactagcact tcataactaa actgaaaacc tatcctcttc
      181 ttttttaatg ttttcatttt tactaatctt tttaatagaa tttctgtagt acttttcctc
      241 cacttatatt tttctttcat tgcttctatt atttcttttt tagataaaat agaatttttc
      301 ctccacaagt attccatcaa catcaattct cccctgcgta gtttattaac tacttcttgt
      361 tgtttcattt tatgtcactc cctcaaattg ttctaatcta aaataataat aggagctttt
      421 tatcacaaaa actacaggta aatttttcca tatattctac gcatttagtc tgttttctta
      481 gaagaaaata actttcatac ctataaattt attcaatttt caaacaatct tgttgaacaa
      541 taactaattc tattgtatta ttttagttgt agaataaaac taaatcggca aaactagaga
      601 aatttagtga cgcaaagcta tagggactaa gacttatata aatatcttat gagttatgtc
      661 agccagttgc caaaaagata ttgttctttt tgtttttatg aaagtttttt agggggataa
      721 taatttatat gtttaaaaat aaaatggata aatgtacaca catgttgact gcttatatta
      781 gcagttcgta tgattattgt aattttttag atacacagtt agatgatttt atattagagt
      841 acggagaaaa tgtagtagag tcttgtttac accaagtgat ggtattggta agtaagtata
      901 attagataga cattgatttc tagagaaccc tttttagttt tatctacttt taaataatga
      961 tatgataaaa aattaattaa aatagattta taatattcat agttttattt tttcttaaaa
     1021 tgcgatataa taaagatgta aattcgtatc aaaaacaaaa aaagaaacta caatctatta
     1081 gcctagagcg aagtttcaaa ttcaaaataa tttaagtaat gatttattca ttgaaatcgt
     1141 cttgattgcc gtcaggacga tttcttagtt tatgtaaaga ttcttttaat ctacttatag
     1201 aatctaataa tcttgttata gactttacta ttgttgtaat agattttatt acaatagtaa
     1261 tgtaatacgc tataacaagt ataaacataa gtgttttcat atactcaccc cctttcgttc
     1321 gaaggtgatt tccgaaaact ttcgccctta gattgtagcc tcttgcaaga tatattcttg
     1381 cattttaatt ataacataat tttacaatta tcaaatatct attctccatt tttttattta
     1441 tacaatatat tctatattgc aataaaaaac attgttttaa atatatatac ataggtatat
     1501 actaaatatg ttaaacttaa ttatggaaaa tataaaatga aaggaataaa aatatgacta
     1561 agactatatt atgtgattac tgtaataaag gaataaataa agatgataat aagtatatta
     1621 cttttcataa gaaaagtcat atgaaaacta acatttgtat taattgtgca ttaaatttga
     1681 tagataaaga taaattaaat gaaaatatta taaataatca acatgattat tcaaagaaat
     1741 gaaaaagcac tctccataat gaagaatgct ctatataata atgtttgact tagtaaagat
     1801 gtgttggggt tacatattta cttttttatt atatcatata actttgtgta tgaaaaagaa
     1861 tttaaatcaa ttttaaggtg tgttgagtaa tgttcttgat agtttatatg ttgatgaatt
     1921 tcaaaaaaat aagcactctt ataaaaagag tacttttggt atatattcaa gcatttatct
     1981 aatacaatta tagcatgtct tatgttttag tatgataatt ttcgttcgtt ttattattac
     2041 aggttatact atagttttat gtatcctgtt aatatttttt actatttttt caatgttttg
     2101 tttaagtata aaaagagata actattttaa ttctagttac ctctttttgt gttactttgg
     2161 ccatatagat atacctattg taatagccat tgtagcaatt cctattatag tagttataca
     2221 taatgcaata atccatttat tagttgaatc aattctatct tctatacctt ttatactatt
     2281 ctttatttca cttacatcct tttctgtcac ctttttatgt tcgtttatag cttcttttaa
     2341 atcgcttttt aactcaattc tttctctagc taaatcattt tttagttcta ttctatcttt
     2401 gtctaaatca ttcttgtatt ctttaaaaat accctttatg tcattaaatt gcttatctat
     2461 actttctttt acttccttat tattttgatg tactcttttt tcagtctcta atattctttt
     2521 ttcagtttct agtattcttt tttcttcttc attcatatta cctacctcct ctgaagtagt
     2581 agctaaaaac caattatctc caatacattt atttttctcc atcaaagtac tatttaaagt
     2641 attataatag tctgtatatt tgttgatttt acttttatcc ttgttggaat taaactttat
     2701 tactttttct ttttctttat tatattcacc cttcataact attcttcctc tatcatagac
     2761 tctaaatgtt caattattga ttgtatttct gatttagata gttctaattc caaagatttt
     2821 ttatcatttc taaatagttt caaaataaaa tcattttctc tagccaaaga agatatagaa
     2881 tataagccaa ttggattgat tctctgaata tatgagttat ctaatatatc atatgtacta
     2941 tttgaaaaag tatacaaatc atttttaaag ttttggctta tatttacatt ttcaccttca
     3001 ttataaaagt ctttcagttt aattccatac tcacctataa attctgggtc ttctcccaaa
     3061 atttcatttg ctttagcttt aaattcatga atacaatgtg gccaagttgg catctgcata
     3121 tataaattaa gtaatagtat tgtcttttca aatattttgt tatccttttc gtattttttt
     3181 atacagttag gaaaatacgc taccaaatta gtcccatttt cataagccat cttaactccc
     3241 cctaaaataa tattatttaa tactattcta cattataatt actttcattg cactattatt
     3301 ttttattcct cctcctacca cttattgcta tatactcaac tgctcttcat atacctgatt
     3361 ttttaaagtt tgtagatgac ttatggttat atttaatata tcatttttta ttttataaat
     3421 tgttgtgata ctgtcataaa attgtagtca aaatgtaaaa tatattaact ctattgattt
     3481 tatttattta taaactccag tgctttataa agtgtatcaa atctatcatt accctttatc
     3541 atagtaaatt tttctttagt catagaacca atcttctcac atgctacacc gcctacaaca
     3601 taaagatttt gcgtctgacc tggcacgtaa tcttttatat cacatatcag tattttccca
     3661 tcattataac cccaaccaac tacagttgca gagattttgt caacttctcc atcataaaca
     3721 attgtatgtt tgtacatctg tttaactccc tcattattta tatttttatt taatacacct
     3781 tctacaat
//
LOCUS       DAMJYR010000229         3621 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.234, whole genome shotgun sequence.
ACCESSION   DAMJYR010000229 DAMJYR010000000
VERSION     DAMJYR010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3621)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3621)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3621
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.234"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            138..1514
                     /locus_tag="PC471_003742"
     CDS             138..1514
                     /locus_tag="PC471_003742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk family potassium uptake protein"
                     /protein_id="HDF2790820.1"
                     /translation="MKTIVRRLILYADGMRPTQIMVSGFAAIIVIGALLLTLPIASQS
                     GESIGLLNALFTATSAVCVTGLVMVDTATYWSLFGQIVIITLIQIGGLGFMTVATMFS
                     LMARKKIQLRERLLIQESLNQADLSGLVRLTRFVLIITITIEGIGALVLSTVFIPQFG
                     LSKGIWYSVFHAISAFCNAGFDLMGSVSGPFTSLNSYVNNFTVSMTVCALIVLGGLGF
                     PVVLDIVRKRRFSKLNVHSKVVLFSTATLIFVGALFIFLIEFNQKATMADLPLKGKVL
                     SAIFQSVTARTAGFNTLDLATLRESSVFVMIILMFIGASPASTGGGIKTTTLAVLIIT
                     VRSFLSGKSDIEAFERRLAPSTIKKSLGIFVISISAVIFGTLIISITQPNFTLVQSAF
                     EVTSALATVGSSLAGTPNLNALGKIIIIIFMFMGRVGSLTLFMAILSGGRRKSQPIRY
                     AEGKIMVG"
     gene            1825..2496
                     /locus_tag="PC471_003743"
     CDS             1825..2496
                     /locus_tag="PC471_003743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HDF2790821.1"
                     /translation="MKQYIVIGCGRFGSSVASTMHLLGHQVMAIDKNEDSVQSISDKV
                     THSLIVDVTDEQALRSLGLGNFDVAVVAIGSDIRASIMATLIAKEMGVELIICKAKDE
                     LQAKVLYKIGADRVVFPERDMGVRVAHNLVSDNILDHIELDPEYSIVEIVTPNSWVGK
                     TLIELELRARYEITVLAIKTGKNINVTPSPDEELTAGSILVIIGQNTSITAITSGNKG
                     IIRRR"
     gene            2685..3482
                     /locus_tag="PC471_003744"
     CDS             2685..3482
                     /locus_tag="PC471_003744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HDF2790822.1"
                     /translation="MITNINSKDNEKLKYTRALLKSKNRNKESKFIIEGYRIVMLALE
                     CMANLDYVFINEEFENKKEHVKLLEDLDKKNTKIYKTTNKNFKELVDTENTQGIIGVV
                     SFKKKKLSESINKKDKFVLILDRIQDPGNMGTIIRTADSAGVDAIIALKGCVDIYNPK
                     VIRSTMGSIFDMNIIDASQDETVDMLKSLDFNIVSSYLNTENFYDKIDYGSKVALVIG
                     NEANGINEELVSKSDILVKIPIYGKAESLNAAISSAILMYEIKKYLI"
BASE COUNT         1368 a          401 c          673 g         1179 t
ORIGIN      
        1 aattataaaa attactatca ttgtctttat acaatattta tactataata gaataataat
       61 ttagaggaaa gctctaaaaa taaacaaatt aagggtctta acagtagtga ttttatgtta
      121 aggaggtata aagaaagttg aaaacaatag ttagaagact tattctctac gcagatggaa
      181 tgcgtcctac tcaaataatg gtatctgggt ttgctgcgat tattgtaatc ggagcattgt
      241 tgttaacact cccgatagcg tcgcagagtg gagagagtat aggcttatta aatgctctat
      301 ttacggctac ttcagcggtt tgtgttactg gtctagttat ggtagataca gcaacttatt
      361 ggagtttatt tggacagata gttataataa cattgataca aattggtggt cttggattta
      421 tgaccgtagc cacaatgttt tcacttatgg cacgaaaaaa aatacaacta agagaaagat
      481 tattaataca agagtcatta aatcaagctg atttatcggg acttgtaaga cttacaagat
      541 ttgtattaat aataaccata acaatagagg gaataggtgc actggtttta tcaactgtct
      601 ttattccaca atttggtttg tctaaaggga tatggtatag tgtatttcac gcaatatcag
      661 cgttttgtaa tgccggattt gacttgatgg gcagtgtaag tggcccattt acatcgttaa
      721 attcttatgt aaacaacttt acagtatcaa tgactgtttg cgcacttata gtgcttggag
      781 ggctaggatt ccctgttgta cttgatattg taagaaaaag aagattttca aaattaaatg
      841 tgcattctaa ggttgtattg ttttcaactg ctacacttat ttttgtgggt gcattattta
      901 tttttcttat agaatttaat caaaaagcta ctatggcgga cttgccttta aagggaaaag
      961 tactatcagc aatatttcaa tcagtaacag caagaactgc tggttttaat acgcttgatt
     1021 tagctacttt acgagaaagt agtgtatttg taatgataat tttgatgttt attggagcat
     1081 cacctgcatc aactggtggg ggtataaaaa caacaacact agctgtttta atcattacag
     1141 ttagaagttt tctatcagga aaatcagata tagaagcttt tgaaagaaga ttagctccat
     1201 caactataaa aaaatctctt ggtatatttg taatcagtat atcagcagtt atttttggaa
     1261 cattgattat ttcaataact cagcctaatt ttacattagt tcaatcagct tttgaggtta
     1321 catcagcact tgctacagtt ggttcaagtt tagctggaac tccaaactta aatgctttag
     1381 gtaagattat aataataatc tttatgttta tgggtagagt tggttctctt actttattta
     1441 tggctatact ttctggtggt agaagaaaga gtcaaccaat cagatatgca gaaggtaaaa
     1501 ttatggttgg ttaagttacc aaggagtagt ataaatttat aatgggttta gagtagcact
     1561 gtaaatctgt tgataaaata aatgagccta gatgtaatta tatgaagaaa taaaatcaac
     1621 taatgaacaa aattaattaa tgaaataaaa aaacagggaa ataaaataca aataaaaagg
     1681 taaatcaaaa ttaaattaat ctgtgtgtat aaaattatat attgtgtata agttttgtat
     1741 gtgtaaagta tataagtttt gtatgtgtaa acaattaatt taagagatat tatataaatt
     1801 gtgtattagg gaggaagttt cgagatgaag caatatatag tcattgggtg tgggagattt
     1861 ggaagttcag ttgcgtctac tatgcatctt ttaggacatc aagtaatggc aatagacaaa
     1921 aatgaagatt cagttcaaag tatatctgac aaggtaaccc attcacttat agtggatgtt
     1981 actgatgagc aagcgttaag gtcattaggt ttaggtaact ttgatgtagc agtagttgca
     2041 ataggttctg atataagggc atctataatg gcgactctta tagccaaaga aatgggtgta
     2101 gagttgataa tatgtaaggc aaaggatgaa ttacaagcta aagttcttta taaaattggt
     2161 gcagatagag ttgtatttcc agaaagagat atgggagtaa gagttgcaca caatttagtt
     2221 tcggataata tattagacca tattgaactt gacccagagt attcaattgt tgaaatcgta
     2281 actccaaata gttgggttgg caagacactt atagagcttg aattaagagc tagatatgag
     2341 ataactgtac ttgctataaa aacaggtaaa aatataaatg ttacaccttc tccagatgag
     2401 gaacttacag ccggaagtat cctagttata atcggtcaaa atactagtat aacagcgata
     2461 acatctggaa ataaggggat aattagaaga agataattta ctatttaata tatatttaat
     2521 tgcaatgaaa gcagatagta tcatataatt atgaatagtt atatgatact atttttttaa
     2581 tcgaaggtag tatttatttg taagattaga taaagagaag ttaaattaaa agtaaggagg
     2641 ctgtgctaat ataaaaattt ataagttatt agcatttaga taaaatgatt acaaatataa
     2701 acagtaagga taatgaaaag ttaaagtata caagagcact attaaaatca aagaatagga
     2761 ataaagagtc aaagttcata atagaaggat acagaatagt aatgcttgca cttgaatgta
     2821 tggcaaacct tgattatgta tttatcaatg aagaatttga aaataagaaa gaacatgtaa
     2881 aactattaga agatttggat aaaaaaaaca caaagatata caagactact aataaaaact
     2941 ttaaagaatt agtggataca gaaaatactc aaggaataat aggtgtagtt tcatttaaga
     3001 aaaaaaaatt aagtgaaagt ataaataaaa aagataaatt tgtattgatt ttagacagaa
     3061 tacaagaccc aggaaatatg gggactataa taaggactgc tgattctgct ggagtagatg
     3121 ccataatagc actaaaggga tgtgtcgata tatacaaccc aaaagtaatt aggtctacta
     3181 tgggttctat ttttgatatg aatataattg atgcttcaca agatgaaact gtggacatgc
     3241 ttaaatcatt ggattttaat atagtttcaa gttacttaaa tacagaaaat ttttatgaca
     3301 aaatagatta tggctcaaaa gtagcattgg tgataggaaa cgaagcaaat ggaataaatg
     3361 aagaacttgt atcaaagtct gatattttgg ttaagatacc tatatatggt aaagccgagt
     3421 cgttaaatgc tgcgataagc tctgctatac tgatgtatga aataaaaaaa tacttaattt
     3481 aatgtattgt aataaatata atgtcatgtt ataatcttga attaaatagt agagtatcaa
     3541 aaatagtcaa tatatattaa aaaaataatt aaattaatta tatattagat gtatattata
     3601 taaaaaataa ttgagttaat t
//
LOCUS       DAMJYR010000230         3602 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.235, whole genome shotgun sequence.
ACCESSION   DAMJYR010000230 DAMJYR010000000
VERSION     DAMJYR010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3602)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3602)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3602
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.235"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            4..738
                     /locus_tag="PC471_003745"
     CDS             4..738
                     /locus_tag="PC471_003745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003722224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2790823.1"
                     /translation="MKNISKDYGIKGFKTNVLKNISLTVNEGDFISIMGPSGAGKTTL
                     LNLMSTLDKQTSGEIILDGINISKVKNNELSKLRREKIGFIFQDYNLLDNMKLMDNIA
                     LPLALGKKKSKEIEAKVFSIAKKFGLENHLDKYPYQLSGGQKQRGAAARSLITNPTVI
                     FADEPTGALDSKSAYELLESLEKINRENNATIIMITHDPLTASYSNEVYMINDGNIKC
                     KLNKGNSRKEFYGKIMDMLASMGGEM"
     gene            739..2646
                     /locus_tag="PC471_003746"
     CDS             739..2646
                     /locus_tag="PC471_003746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HDF2790824.1"
                     /translation="MSILGIAYNNFKSNIRTYLAFFISMVFSVVVLTNFELLRYGDAI
                     NVLQDENKKFTLSVLVAVIMILSVFLFFFIWYATNIFFKNRSKEIGILSFMGLDLYTI
                     GKIYFVENMLIGISSCITGIIIGIITSRFFQVVIIKLSGFNIKVSNGLSIEAILYATL
                     IFISIFIIMAVKGFITICRSSVINLINISKKQEKIPKVGIGIYILAIVSIIVIGYGYY
                     LSTNIRTGDITSVLPVIGIIIVGTYGLFKSVMPVIFDIIIKNKKILFNGNNIIAINNI
                     NYRLNKNYKTYAIIAIIITTTISTLGASVAMKRIHKNAQEQRNVYTISIVSMDKDEVN
                     KKQIENIIKDTNKIKYSVNPELTILKKTDIKEAYDTTDTIMKYSDFCNTLKVNGNDKE
                     LKEYGERLVSGNNVIELKSPQTIASIATKEAKIKLDGQLYNISRGEVKVPVLGTGLNQ
                     SIIVVNDDTYFKLRDKGQQVYFYGAKVFQEENSQVMFNNIEKNLSLKNGYIQNGYKAQ
                     GESQWMKFAYVVLVFLFLVFAIVAGSIIYMKIYSDAYEDKEKYKILLKIGTTEKDINR
                     AILKEVAMFYTLPMLSATISSYFALRLAGDLLMTDLFGIYILSLVICLIIFIIYGAVS
                     VNKFKSVVYKN"
     gene            2798..3478
                     /locus_tag="PC471_003747"
     CDS             2798..3478
                     /locus_tag="PC471_003747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2790825.1"
                     /translation="MYRILLVEDDIDLSKEIALALEKWGFKVELIDDFEVVLDEFIDK
                     KPDVVLLDVNLPLYNGFYWCEKIRAISNVPLIFLSSRDSDMDLIMGINNGADDYITKP
                     FSIEILVTKINGIIRRVYNYSDSNSILYCEDLMFDVGKGIIKHKYKDKSIELTKNEIK
                     ILTLLLKNKNRVVSRESLMMTLWDNDEFVTDNALTVNMNRLRSKVKELGFDDFIKTKK
                     GVGYIIQC"
BASE COUNT         1498 a          320 c          608 g         1176 t
ORIGIN      
        1 aaaataaaaa atatatcaaa agattatgga ataaaaggat ttaaaacaaa tgtgttaaaa
       61 aatatatctc ttacagtcaa tgaaggtgat tttatatcaa taatgggtcc atctggtgca
      121 ggaaagacaa cattgctaaa tttaatgtct acacttgaca aacaaacttc aggagaaata
      181 atactagatg gaattaatat atcaaaagta aaaaataatg aactatcaaa attaagaaga
      241 gaaaaaatag ggtttatatt tcaagactat aatctattag acaatatgaa actaatggac
      301 aatatagcac ttcctctagc attaggaaaa aagaaaagta aggagataga agctaaggta
      361 ttttctatag ctaaaaaatt tggattagaa aatcatttag ataaatatcc atatcagtta
      421 tcaggaggac aaaaacaaag aggcgcagca gctaggtcat taattacaaa tccaacagtc
      481 atatttgctg atgagccaac tggtgcttta gactctaaat cagcatatga acttttagaa
      541 tcattagaaa aaataaacag agaaaataac gctactatta taatgattac acatgaccct
      601 ctaacagcaa gttattcaaa tgaagtatat atgataaatg atggaaatat aaagtgtaaa
      661 ttaaacaaag gaaatagtag aaaagaattt tatggaaaaa ttatggatat gctagcctct
      721 atgggaggtg agatgtaagt gagtatactt ggaattgctt ataataattt taagagtaac
      781 ataagaactt atctagcatt ttttatatct atggtttttt cagtagttgt gctaactaat
      841 tttgaattat taagatatgg tgatgctata aatgtgttac aagatgaaaa taaaaaattt
      901 acactatcag tgttagtagc tgtaataatg atactatcag tatttttatt tttctttata
      961 tggtatgcaa caaatatatt ttttaagaat aggtctaaag aaattggaat attgtctttt
     1021 atgggattag atttatatac tataggaaaa atatattttg tagaaaatat gcttatagga
     1081 ataagttctt gtataacagg aataattata gggataataa catctagatt ttttcaagta
     1141 gtgataataa agttatctgg atttaatata aaggtatcta atggacttag tatagaagct
     1201 atactatatg ctactttaat ctttatttct atatttataa taatggctgt aaaaggtttt
     1261 ataacaatat gtagaagtag tgtaataaat ctaatcaata tatctaaaaa acaagaaaaa
     1321 ataccaaagg taggaatagg tatatatata ttagctatag tatcaattat agtaatagga
     1381 tatggctact atctttctac caatataaga actggagata taactagtgt actacctgtt
     1441 ataggtataa taatagtagg tacttatgga ttattcaaat cagttatgcc tgttatattt
     1501 gatattataa tcaaaaataa aaaaatatta tttaatggaa ataatataat tgcaataaac
     1561 aatataaact accgattaaa taaaaactat aaaacttatg ctataatagc tataattata
     1621 actacaacaa tatcaactct tggtgcttca gttgcgatga agcgtataca taagaatgcc
     1681 caagaacaaa gaaatgtgta cactattagc atagtttcaa tggataaaga tgaagtgaat
     1741 aaaaaacaaa tagagaatat aataaaagat actaataaga taaagtactc agtaaatcca
     1801 gaactaacaa ttttaaagaa aacagatata aaagaagctt atgatacaac agatactata
     1861 atgaaatata gtgatttttg taatactctt aaagtgaatg gcaatgataa agaattaaaa
     1921 gagtatggtg aaagattagt tagtggaaat aatgtaatag agttaaaaag ccctcaaacg
     1981 atagcatcaa tagctacaaa agaagccaag ataaaacttg atggtcagtt atataatatt
     2041 tcaaggggag aagtaaaagt tccagtacta gggacaggac ttaatcaaag tattatagtg
     2101 gtaaatgatg atacatattt taaactaaga gataagggtc aacaagtgta tttttatggt
     2161 gcaaaggtat ttcaagaaga aaatagtcaa gtaatgttca acaatattga aaagaattta
     2221 agtttaaaaa atgggtatat tcaaaatgga tataaagctc aaggtgagag tcaatggatg
     2281 aagtttgcat atgtagtgct tgtgttttta ttcttagtct ttgcaattgt agctggaagt
     2341 ataatatata tgaaaatata tagtgatgca tatgaagata aagagaaata taagattttg
     2401 ttaaaaatag gtactacaga aaaagatata aatagagcta tacttaaaga agtagctatg
     2461 ttttatactt tgccaatgtt aagtgcaact atatctagtt attttgcatt gagattagca
     2521 ggagatttat tgatgaccga tttatttggt atatatatac taagtttagt gatatgtttg
     2581 ataatattca taatatatgg agcagtatct gttaataagt ttaagagtgt agtgtataaa
     2641 aattagttga tatattaata aagagaaagg agattcttgt aataagagtc tccttgttga
     2701 ttaactaaat aatttgggta ataaattcat ataaaatatg ttatgatatt aataattcat
     2761 agcatagtga aatattagga taatggagaa tatacaaatg tatagaattt tattagtaga
     2821 ggacgatatt gatttatcaa aagagattgc attagcactt gaaaaatggg gatttaaagt
     2881 agaattaatt gatgattttg aggtagtatt agatgaattt atagataaaa aaccagatgt
     2941 agtactttta gatgtaaatt taccattata taatggattt tactggtgcg aaaaaataag
     3001 agctatttca aatgtaccat taatattttt atcatcaaga gattctgata tggatttaat
     3061 aatgggtata aataatggag cagatgatta cattaccaaa ccattttcta tagaaatatt
     3121 ggttaccaaa ataaatggta taataagaag agtctataat tatagtgatt ctaatagtat
     3181 tttgtattgt gaagatttga tgtttgatgt ggggaaaggg attataaaac ataagtataa
     3241 ggataaaagt atagagttga ctaaaaacga aataaaaatt ttaacattat tattaaaaaa
     3301 taagaatagg gttgtatcaa gggaaagtct tatgatgact ctatgggata atgatgagtt
     3361 tgtgacagac aatgcactta ctgtaaatat gaatagattg agaagtaaag ttaaagaact
     3421 aggttttgat gattttataa aaactaagaa gggggtagga tatataatac aatgctagta
     3481 aaatatttga aaggtaggtc taagtatatt gctctacctt tagttgtagt tagtataata
     3541 aatatctact tatttgctat agatattttt aaaaataaat attcagagtt aatatattta
     3601 ga
//
LOCUS       DAMJYR010000231         3594 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.236, whole genome shotgun sequence.
ACCESSION   DAMJYR010000231 DAMJYR010000000
VERSION     DAMJYR010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3594)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3594)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3594
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.236"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(78..992)
                     /locus_tag="PC471_003748"
     CDS             complement(78..992)
                     /locus_tag="PC471_003748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II restriction endonuclease"
                     /protein_id="HDF2790826.1"
                     /translation="MELVKNIHKNKSKDEIFKELLENLKGTIASWDYYVNWNKALENT
                     RKIEVQLNILNYLIGKEDIENETKNLLKEYPEVLSVIPIVIASRDNEFKILDPSDEDM
                     FKYKLFNFKKRKSISDEESELATEFLKKIKFLDLLKQKNIKNLVDYVFGVEVGLDTNA
                     RKNRTGSSMETLVERYVKNMCEKNNWSYLTQATVKKISEKWSIEVPTDKSKRKYDFAI
                     NNNGKIVLVEVNFYNGGGSKLKSVANEFTKLDKYIKDGGFTFCWITDGLGWLESSIPL
                     RDAFDKMDYIFNLDMVDKGSLEYIIKNL"
     gene            complement(1011..1925)
                     /locus_tag="PC471_003749"
                     /pseudo
     CDS             complement(1011..1925)
                     /locus_tag="PC471_003749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008909111.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA adenine methylase"
     gene            complement(2353..2702)
                     /gene="ssrA"
                     /locus_tag="PC471_003750"
     tmRNA           complement(2353..2702)
                     /gene="ssrA"
                     /locus_tag="PC471_003750"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(2860..3315)
                     /gene="smpB"
                     /locus_tag="PC471_003751"
     CDS             complement(2860..3315)
                     /gene="smpB"
                     /locus_tag="PC471_003751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HDF2790827.1"
                     /translation="MAGKKILATNKRARHEYFIEETYECGIELKGTEVKSIRQGKVNL
                     SDGFASVDNSEVFLKQVHISPYEQGNIFNVDSLRVRKLLLHKHEIRKLIGATTIKGYS
                     LIPLSLYLKNGKVKVEIALAKGKKLYDKRQDMAKKDAQRRIEREMTGKY"
BASE COUNT         1184 a          646 c          341 g         1423 t
ORIGIN      
        1 ctcaaatatt ttatataata tataagtcta aattaacttg caatataaaa gcgcctctta
       61 atataggcgc tataatgtta taaattttta attatatatt ccaatgaacc tttatcaacc
      121 atatctaaat taaatatata atccatctta tcaaatgcat ctcttaatgg aatacttgat
      181 tcaagccatc ctaacccatc tgtaatccaa caaaatgtaa aacctccatc ttttatatac
      241 ttatctaact tagtaaactc atttgcaaca gattttaact tagaacctcc accattataa
      301 aaattaactt ctactaaaac aatttttcca ttattattta tagcaaagtc atactttctt
      361 ttactcttat ctgttggaac ttctatagac catttctcac ttattttttt cactgtagct
      421 tgtgttaaat aactccaatt attcttttca cacatattct ttacatatct ttctacaaga
      481 gtttccatac tacttccagt tctatttttt ctagcattcg tatcaagccc aacctctact
      541 ccaaacacat aatctactaa attttttata tttttttgtt ttaataaatc taaaaacttt
      601 atttttttaa gaaattctgt agctaattcg ctttcttcat cacttatact ctttctcttc
      661 ttaaaattaa acagtttata tttaaacata tcctcatctg atggatctag tattttgaat
      721 tcattatctc tactcgctat tactattggt atcacactaa gaacttctgg atactctttt
      781 aataaatttt tagtttcgtt ttctatatct tcttttccta taagatagtt taagatattt
      841 aactgaacct ctatctttct agtattttcc aaagctttat tccagttaac ataataatcc
      901 cagctagcta tagtaccttt taaattttca agtaattcct taaatatttc atccttactc
      961 ttatttttat gaatattctt aaccaattcc atttgattcc ccacctcttc ataattagta
     1021 attagcaatt cagttataaa acctcttcca ttccccttag aatttatgaa tcttttagca
     1081 tgtactcttt ttatattaaa atctgaatac aaatcatcaa agaaagtatc ttcttcatct
     1141 aaaacttttg gatcagaatt acttaacatc aattttgtat ctattttatc tagaacttta
     1201 taaaatttac ttaactcaat ttgactatca tcattaaaat tacctttgtt ataggaatta
     1261 aatcctccta tagttactgg tctatatggt ggatctagat acacaaaaca atttttatct
     1321 attatatttt ctagatttaa ataattctta tttaatattt ttacttttag ctctcctcga
     1381 gaatctcttt tatttaaaag catggataat ttttcaagtt catttttatc aaaaaaacta
     1441 ggatttttat atcttccaaa aggtacatta aattcacctc gagaattttc tctatataat
     1501 ccattaaaac atgttttatt taaaaatata aaatatgaag cttgctttat acaatcttca
     1561 ttttctatat ttttttcatt tgtaagtata ttaaatctat ctctaatatc atagtacatc
     1621 ttttctctat ctgaagaacc ttcttcgtaa gatatgtatt gttcttttaa attagttagt
     1681 tctgttatta actcttccaa atcatctctg actttaatat ataaattaat taacttacta
     1741 tttatatcat taattacata cttgttgaaa atgtcctctt ctaacaaata tagaaatagt
     1801 gcacctgcac ctacaaaagg ttctatataa gtcatggaac taaaatcaca acctgtaggc
     1861 atttgttttc ttagaatatc aagtagctgt gttttacctc cagcccattt taaaattggt
     1921 ttcatgcaac acctctctca gttatattgt tatataatta tataaaataa gattctaaaa
     1981 tacaagtact catcagaaca ctatatttat atcaaatctt tccaaaatct aagtattatt
     2041 ttatcctaac acttttcaaa acctctaaat aatatacaat attctctaat atttacttac
     2101 taaaaattta aatctatctt taagtataac ctaaaacaga ttataagtta cgtagttttt
     2161 aatataaaat atacttctac aagtaaagct ttaaatatca attacttatt tcataattaa
     2221 tgactatgta ccctcaaaaa aacctcattt tctatggttg gggtacggtt ggtggatttc
     2281 atcaccaaaa taaaaaatac tagtcaataa agactagtat ttccaagtat ttcatcattc
     2341 ttttttcaaa attggtggag acgaggggag tcgaacccct gtccgcaaat cttttccttg
     2401 ctgcatctac atgcttagcc aacttcacta atatctcacc tcacatagcg ccaagtggca
     2461 ggctctatgc ttagccagtc ccaattaatc tacttcaggt cgagacaccc ctaaagtagt
     2521 tccctgcata atgacgtcca gtcttagtcg gcaggaggac taagagggac gagcagcaac
     2581 taggctgcta tcgcgaaatt atcgtctgcg tttattttaa agttccccag tttttacgtg
     2641 acccagagta acacgacatg cagcctcaag ttccaaaccc acgtcgaaac ctttacgccc
     2701 ccttgagtag tttatagtcc agtagacttt tcgcaaactt tatcaccatt tttattaaat
     2761 ttttaatgta tatataatat aatactttta agaataaaaa tcaatgttat gttctagata
     2821 ataaagaatt ttattctaaa aaatacagtt ttatctagtt taatacttac ctgtcatttc
     2881 tctttctata cgtctttgtg catctttctt agccatatct tgacgtttat catatagttt
     2941 tttacctttt gctaaggcaa tctctacttt aactttaccg ttttttagat acaaactaag
     3001 tggtataagt gaatacccct ttatagtagt tgctccaata agctttctta tttcatgctt
     3061 atgaagtaat agttttctaa ctcttaaaga atcaacattg aatatatttc cttgctcata
     3121 tggacttata tgaacttgtt ttaaaaatac ttcgctatta tctacagatg caaatccatc
     3181 acttaggtta actttgcctt gtcttattga ttttacttct gttcctttta attcaattcc
     3241 acattcataa gtttcttcta taaaatattc atgtcttgct cttttgtttg ttgctaatat
     3301 ttttttacca gccatataaa tctcaccttc tttgctagaa atttattcca taaatattag
     3361 aaatttacta aatatttaat gttgccaaat tatttgacta acttgtatga taacatattt
     3421 aggcatttta gtcaatattt cactgatata ttaagttagt taggctatat ataattaagt
     3481 aatataaatg tatgaaaata gctaaaaaac tacttcgaat atacttaata ctaaactaac
     3541 tctaaccagt aatactaatt tataaatttc aaaacaaaaa tattacatat tata
//
LOCUS       DAMJYR010000232         3333 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.237, whole genome shotgun sequence.
ACCESSION   DAMJYR010000232 DAMJYR010000000
VERSION     DAMJYR010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3333
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.237"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(273..530)
                     /locus_tag="PC471_003752"
     CDS             complement(273..530)
                     /locus_tag="PC471_003752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDF2790828.1"
                     /translation="MKIKVEDIPVQFHAMVEIIGIDKFIEVAKLYGGTNTYIPTYKGL
                     FRYARNRDIVKQFNGVNHNELAIRYNMCVSNIKRILNENSV"
     gene            1110..1331
                     /locus_tag="PC471_003753"
     CDS             1110..1331
                     /locus_tag="PC471_003753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1659 domain-containing protein"
                     /protein_id="HDF2790829.1"
                     /translation="MEVVSTKNLSSIKLKLDAGFDDKGKAVVKSKSFANVKAEALNED
                     VYAVAEAIASLQENPIVDILKLDSTSLSK"
     gene            2190..2420
                     /locus_tag="PC471_003754"
     CDS             2190..2420
                     /locus_tag="PC471_003754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2922 domain-containing protein"
                     /protein_id="HDF2790830.1"
                     /translation="MEIKKKLVMSFKNVKDKQVSFSIENPKDDLTEENIKSVMDLIVS
                     KNIFSVGGFDLASVVEAKVVETNTTPYDLVIE"
     gene            2433..2582
                     /locus_tag="PC471_003755"
     CDS             2433..2582
                     /locus_tag="PC471_003755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvrJ family protein"
                     /protein_id="HDF2790831.1"
                     /translation="MDDSLQLLITNVGFPIALSMYLLVRIEGKLNTLSNSIDTLSKNI
                     LIFKK"
     gene            2839..3276
                     /locus_tag="PC471_003756"
     CDS             2839..3276
                     /locus_tag="PC471_003756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family transcriptional regulator
                     DNA-binding domain-containing protein"
                     /protein_id="HDF2790832.1"
                     /translation="MKIHVEQGINCDDIEITIKCSALDNNIEKIISLLSIPLLDIKGK
                     KSGEIYKLNVCDILYFETVGNKTFAYYEDDVFEIDLKIYKLSKLLANTSFSRISKSMI
                     LNMDYIKSIKVISHNRMQATLKNNEKVIINRRYIKNIKEKLNI"
BASE COUNT         1352 a          428 c          360 g         1193 t
ORIGIN      
        1 atagtttatt tatttatttt tcccctttat acaagcaatt gtatcatttt atctattttt
       61 tagccaacaa atattttcga ccattttctt tgtattctca atatatctcc agtatttaac
      121 aaattttttt atagaatata caatgtattt aaattaataa gtagtaaatt tagttaattt
      181 gtctaaaaaa actttataaa acatcttctt tgtctttact cttacgttta acaaggctat
      241 tttcattatg tttaaacatt attttattat atttaaacac tattttcatt gagtattcgc
      301 ttaatattgc ttacacacat attatatcta atagccaatt cattgtggtt gactccatta
      361 aattgtttta ctatatccct atttctagca tatctaaaaa gccccttata cgtaggtata
      421 taagtatttg ttcctccata caacttggca acctctatga acttatcaat tccgattatt
      481 tctaccattg catgaaattg aactggtata tcctccactt ttatcttcat aagttacacc
      541 tcctttaaga tatttttata gacttaacaa agtttttatt taatacatta ttgactaaac
      601 tatatttatt agttttattg aatctaataa aaaatttctt agctttaaaa ttaatttttg
      661 tattaacttc aataaaattt ctcataactt ataactttgt tagctttaat aaagatttta
      721 atggtttaaa agtaaaatgt taatattgga cttttaactt ttttatttac agattaaatt
      781 taataaaatt tttaacataa gctttaacat ttaatttaac ataaacttta gtacctaaca
      841 tttaactcta cacatatgaa aatccataca aaactatctc aactaagcat gaatatatac
      901 aaatactatt tcaaaacact aagaacatat cttcacaaat aacttgtaaa ctttttcatc
      961 agcaaaaaag taaaattttt cctataaaaa tttacacaaa aaatctttag aaattactgt
     1021 ttaactccga tattatctta gatgatagta ttatagctaa caaagaaagt cgacaaatct
     1081 ttgaaattaa atttttagga ggtaatttta tggaagttgt ttcaacaaaa aatttatcta
     1141 gtataaaact aaaattggat gctggttttg atgataaggg taaggcagtt gttaaaagta
     1201 aatcatttgc aaatgtaaaa gcagaggcat taaatgaaga tgtttatgct gttgcagaag
     1261 caatagcaag tcttcaagag aaccctattg tagatatttt aaaattagat agtacatcac
     1321 tatctaaata gtgtctttta aacgtactct gctttacaaa ttaaaaaatt aactctttat
     1381 ataaaagatt aagttatata atgcacttaa tacgctatag atttggcaga ttataacttc
     1441 aattttggtt ataactttaa tatattaacg ctttgaaaaa ttaatatatt aaacgtattt
     1501 caacttaaac atgttagcta ttttagatgc taccctactt taatctaatt aattgaaata
     1561 aattaaatat tttctatatc agcaataact agagtcaaca attaaaatac taatattgac
     1621 aattgaaaca ctgatactaa caagcaaaac actaatattg acaattgaaa tactaatact
     1681 aacaagcaaa acattaatat tgacaattga aatactaata ctaacaagca aaacactaat
     1741 attgacaatt gaaatactaa tactaacaag caaaacacta atattgacaa ttgaaacact
     1801 gatactaaca aacaaaacac taatattgac aattgaaata ctaatactaa caagcaaaac
     1861 actaatattg acaattgaaa tattaatact aacaagcaag acactaatat tgacaattga
     1921 aatattaata ctaacaagca aaacactaat attgacaatt gaaatattaa tactaacaag
     1981 caaaacacta atattgacaa ttgaaacact gatactaaca agcaagacac taatattgac
     2041 aattgaaata ttaatactaa caagcaaaac attaatattg acaattgaaa tattaatact
     2101 aacaagcaaa acattaatat tgataattaa aataccaata ttaacaagca aacaattaaa
     2161 atataaatta ttatatcgga ggtaattata tggaaattaa gaaaaaatta gtcatgagct
     2221 ttaaaaatgt aaaggataaa caagtttctt tttctataga aaatccaaag gatgatttaa
     2281 ctgaagaaaa tataaaatct gtcatggatt tgatagtatc taaaaatatt ttttcggttg
     2341 gaggatttga cctagcttct gttgttgaag ccaaggttgt ggaaacaaat accactcctt
     2401 atgacttagt tatagaatag ggggctaatt ttatggatga tagtttacaa ctgttaataa
     2461 ccaatgttgg ttttcctata gctctatcca tgtacttgtt ggttagaatt gagggtaaat
     2521 taaatacttt atcaaacagt attgatacat tatccaaaaa tattttgata tttaagaagt
     2581 aatttgacaa tatatgtaag tcataaaata ggtctatttg taaaataatt agacatattc
     2641 tatgtgaaat actaggatat ttggataatt tatccaaata tccatagtat ttttttgaat
     2701 tttaccacct caaatttgaa tttcactgtt ttatttttga atttcactat gcttaatttg
     2761 aaatttagaa cgcatctagt tggactcaat aagatataat gctattctaa tgacagtaat
     2821 aaaatgaggt gatgattaat gaaaatacat gtcgaacaag gtattaattg tgatgatatt
     2881 gaaataacca taaaatgttc tgctctagat aataacattg aaaaaataat ttcattattg
     2941 agtataccgt tacttgatat aaaaggtaaa aaatcaggag agatttataa actcaatgtc
     3001 tgtgatattt tatactttga gacagttgga aataaaacat ttgcttatta tgaagatgat
     3061 gtatttgaaa ttgatttaaa aatatataaa ttatcaaaat tattagctaa tacatctttc
     3121 tcacgaatat ctaaatccat gattttaaat atggactaca taaaaagtat aaaagtaata
     3181 tcacataatc gcatgcaagc tactttaaaa aacaacgaaa aagttattat aaatcgtcgt
     3241 tatataaaaa atattaaaga aaagctaaac atttaataaa aatctaaagt aatttattaa
     3301 tatcactaat tgcaaattta ttgacctatt taa
//
LOCUS       DAMJYR010000233         3229 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.238, whole genome shotgun sequence.
ACCESSION   DAMJYR010000233 DAMJYR010000000
VERSION     DAMJYR010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3229)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3229)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3229
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.238"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            864..1625
                     /locus_tag="PC471_003757"
     CDS             864..1625
                     /locus_tag="PC471_003757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896901.1"
                     /note="N-terminal region resembles flavodoxins. C-terminal
                     ferrodoxin region binds two 4Fe-4S clusters; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EFR1 family ferrodoxin"
                     /protein_id="HDF2790833.1"
                     /translation="MIGIYFSGTGNSRYCVEKFLQEYDITANSYSIEDNELLQHINNH
                     ENIIFSYPVQYSNVPKILKDFIINHSTLWNGKRVFIIATMALFSGDGSGVLARILKKY
                     GAITVGGLHVKMPDSIGDEKVLKHSLEYNRKLVIKAEKKVTKAAKKLKHGSPPKEGLG
                     FLSHIVGLFGQRLYFINKTNMYTDKLKINTEECIVCGKCVNLCPMKNLVIKNCMIVAN
                     GQCTMCYRCVNNCAKKAITLLGKKVVEQSNIKKYM"
     gene            1690..2472
                     /locus_tag="PC471_003758"
     CDS             1690..2472
                     /locus_tag="PC471_003758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HDF2790834.1"
                     /translation="MEYYIVDSFATKLFKGNPAGVCVLDRRIPLELMQKIAEENNLPE
                     TAFVVKCKGNYELRWFTPKAEIDLCGHATLAAAYVISNFIDVNVKRIDFFTQSGKLEV
                     TRNGNLYEMIFPEIMPIEIELSPQQANLIGCVPSAVYSSRDLILLLNSEQEVINYKPN
                     YAQLRKLTDWLGIIITAQGSNTDFVSRYFCPELDSEDPVTGSSHCNLIPYWSEKLGKH
                     KMVAAQLSNRGGIIQCEVLKDNTVKISGEAVLFMQGTIKIDI"
     gene            2739..3158
                     /locus_tag="PC471_003759"
     CDS             2739..3158
                     /locus_tag="PC471_003759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjdF family protein"
                     /protein_id="HDF2790835.1"
                     /translation="MDKISGKLTVLFEEPFWVGIFERQDGKKYEACRVVFGAEPKEVE
                     VYEFILERFFSLDFGSIKLEKNVTKDNIGYKRMQRKVKKEQEKETIGTKAQNALKLQY
                     EERKQDRKNLAKNRKEEEKERLFNLKQEKRKAKHKGH"
BASE COUNT         1310 a          317 c          568 g         1034 t
ORIGIN      
        1 aacaagtaat gttattaaaa ttaagtatat agttaaaagt gtatagcaaa aaggctgtta
       61 tattaatgtt agaggttcag ggaaaatata taaagcaaag atatttacag ataatgtaga
      121 ttgcacagct ataagttaga taatagaatt atgtaatcaa gaatttacaa caaagtataa
      181 gataaggata atgctaaatt gccatacagg tgctagatgt gtaaatggat ttaattaata
      241 ctattgataa agtgcaacaa gcacataaac caattgatga gattattgaa actataagag
      301 atatagttga cataattaaa atattggaac ctgtttataa ttttaaatca aatcaataat
      361 gattaagatt aaaataatat taaggcatca atgttataag agtacaatat aaggagtact
      421 atatagtact ccttacttat acatattagg caatagggaa tttttttata gttaaatttt
      481 caaatatttt ttagggaact ttataattga gaattaaggg tatagtatgt gatgaactta
      541 ctaaatttat aattaggtta tataatggaa taatcatcaa tagttttact ttagggaaaa
      601 tatatgctta aggaagtata gcttactaat attattaaaa tgtatattta aaatgataaa
      661 aaaagaatta gacaagtatt tagtaaattg actatgcaaa attctatcaa gcaggaacac
      721 tatgacttta gtcatgggag gttcagtagg taacttgttg aattaagttt atattattta
      781 aataaactac ttaatttaga taatataaaa agtcagaatt ttaagaatag agctatataa
      841 tcttagctat taggagaagt actatgatag gaatatattt tagtggaaca ggaaattcaa
      901 gatactgtgt agaaaaattt ttacaagaat atgatataac agcaaattct tattctattg
      961 aagataatga acttttacag cacatcaata atcatgagaa tattattttt agttatcctg
     1021 tgcaatatag taatgtgcca aaaatattga aagattttat aataaatcat tcaactttgt
     1081 ggaatggtaa acgtgttttt attattgcaa ctatggctct atttagtgga gatggctctg
     1141 gtgtgttagc tagaatatta aaaaaatacg gtgctataac tgttggtgga ttacatgtga
     1201 agatgccaga tagtatagga gatgaaaagg tattaaaaca ttctttagag tataatagaa
     1261 agcttgtaat aaaagcagaa aagaaagtaa ctaaagctgc taaaaaactg aaacatgggt
     1321 caccaccaaa agaagggttg ggttttttga gtcatattgt agggttattt ggacaacgat
     1381 tgtattttat caataaaaca aatatgtata cagataaact aaaaattaat actgaggaat
     1441 gtattgtatg tggtaaatgc gttaatttat gtccaatgaa gaatctagta attaaaaatt
     1501 gtatgattgt tgcaaatggg caatgtacaa tgtgttatcg ttgtgtaaat aattgtgcca
     1561 aaaaagcaat tacattgtta ggtaaaaaag ttgttgaaca gagtaatatt aaaaaatata
     1621 tgtaagaaat ttaaagtttt attgaatgta aattgatgta tattttatgt tagaaagaga
     1681 ggttaaataa tggaatacta catagttgat tcatttgcaa ctaaactatt taaaggaaat
     1741 cctgctggag tttgtgttct tgacagaaga atacctttag agttaatgca gaaaatagca
     1801 gaagaaaaca atttaccaga aacagcattt gtagttaaat gtaaaggaaa ttatgaatta
     1861 cgatggttta ctcctaaagc agaaattgat ttatgtggtc atgcaacttt agcagcagct
     1921 tatgtaatat caaattttat tgatgttaat gtaaaaagga tagatttctt tactcaaagt
     1981 ggaaagcttg aagttacacg aaatggaaat ctatatgaaa tgatatttcc agaaataatg
     2041 cctattgaaa tagagttatc accacaacaa gctaatttga taggctgtgt accaagtgct
     2101 gtttattctt caagagattt aatcttattg ctaaacagtg aacaagaagt tataaattat
     2161 aaacctaatt acgcacaatt gcgtaaattg acagactggt taggtataat aatcacagca
     2221 caaggaagta atacagattt tgtatcaaga tatttttgtc cagaattaga ttcagaagac
     2281 cctgttactg gttcatcaca ttgtaatctt attccatact ggtctgaaaa gttgggaaaa
     2341 cacaaaatgg tagctgctca actttctaat cgtggtggta taattcagtg tgaagtgttg
     2401 aaggataata ctgtaaaaat ttctggtgaa gcagtcttgt ttatgcaggg aacaataaag
     2461 attgatattt aaataagtat aataactaat ttattatgaa aaataattaa ttttcttagt
     2521 gttaacaata ataaagtaga gttaaaaagc ttgatttcac ttaaatcaat atgatagatg
     2581 ttgatactaa gattgacaag catgattaaa aaagataata tttaatctat aaagataaaa
     2641 acacagtctg gttggtagtc cagacgcagc aatagctgtc agtaacctgc ctccttggtt
     2701 gtccgttctt tagaaagtaa tttaaggagg aactagcaat ggacaaaatt agtggaaaat
     2761 taacagtttt atttgaagaa ccattttggg taggtatatt tgaaagacaa gatggtaaaa
     2821 aatatgaagc atgtagggta gtttttggag cagaacctaa agaagtagaa gtgtatgaat
     2881 ttatacttga aagatttttc tcattagatt ttggtagcat taaattagaa aaaaatgtga
     2941 ccaaagacaa cattggatac aaaagaatgc agagaaaagt aaaaaaggaa caagaaaaag
     3001 aaactatagg gactaaggct caaaatgctc ttaagcttca atatgaagaa agaaaacagg
     3061 atagaaaaaa cctagctaaa aatagaaaag aagaagaaaa agaaagacta tttaatttga
     3121 aacaagaaaa gagaaaagct aaacataagg gtcattaatt ttaattaaat atctttttaa
     3181 taattgctag aaaaaggtgt ataaaatttt taattttata caccttaat
//
LOCUS       DAMJYR010000234         3211 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.239, whole genome shotgun sequence.
ACCESSION   DAMJYR010000234 DAMJYR010000000
VERSION     DAMJYR010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3211)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3211)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3211
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.239"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            <1..450
                     /locus_tag="PC471_003760"
     CDS             <1..450
                     /locus_tag="PC471_003760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015984839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HDF2790836.1"
                     /translation="EKNIIIFGQGVNPESQNTGDKSGVALKFLYSLLDLKCSKTEKKF
                     KKAIRELLWFVCEYLKISGSKSYDYKTVQITFNHSMIINEAEKIDMAAKSTGIISDET
                     IVSNHPWVEDVNDELDRLKKQKEESIKEYEDAFPTKKKTEEGEPLDE"
     gene            443..1891
                     /locus_tag="PC471_003761"
     CDS             443..1891
                     /locus_tag="PC471_003761"
                     /inference="COORDINATES: protein motif:HMM:TIGR01641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HDF2790837.1"
                     /translation="MNNINYWIKTFNQLEEDIHNKGDNFNKELEKQYDIILYNILREI
                     NNWYMKFAKDNKISMQEAEKLLNSKELTELKLSLEEYIKYGEENVISQKWMKELESAV
                     KRVRISRLEALKLKIRHQVEVLYSKEYEDVNTLITNTYKDSYYHTAFEAQKGIGIGLM
                     IAMLSMNNINKIVSSPWTTDGITFGNRIWNKHRPRLISELNKGLKQTLINGVSPENLT
                     NKINKNFNTSKEEAKLLVKSELAFFSSLSQRDCFNSLGVEKYEIVSASDSRVCEKRCS
                     PLDGKVIEMKYYEIGITAPPFHPRCRCVVVPYFDGEESYRAAREEDSETYYVPASIKY
                     NEWYSKYVKDSFSSGAISRKRLKDLEIYLREEKHANLYYEEIRKRTSDIKAIAKNTGY
                     SEKVIQDIKSHVFMNKYNLYGGYKSFDPSYDMAISWQRLIEGKDIRKCDIILLKHERL
                     EKFLMDRYNYDYKKAHGLVVRKYDYEKALREG"
     gene            1894..2136
                     /locus_tag="PC471_003762"
     CDS             1894..2136
                     /locus_tag="PC471_003762"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2790838.1"
                     /translation="MVLIKKNYIKDDKVSYFYQPEEDGEFGEVILYTKDKSFDIVKIA
                     ERDSEGNHFYMSHVYCMIRKFIEENNYPDEKTSYWY"
     gene            2207..2401
                     /locus_tag="PC471_003763"
     CDS             2207..2401
                     /locus_tag="PC471_003763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015981492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2790839.1"
                     /translation="MCILTQVIVVVCVVQIVINCIANLNVGILCNKLKKENKANIDKV
                     SDEILKRVREELNKSLDKSL"
     gene            2443..2673
                     /locus_tag="PC471_003764"
     CDS             2443..2673
                     /locus_tag="PC471_003764"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2790840.1"
                     /translation="MKEYIIWFKSGNCVSGITDEYVADKLMKDFMGADSDCRNLKGYL
                     DEDGTTIIDLSQIEAMSINNCNENNNIGFSKS"
     gene            2718..>3211
                     /locus_tag="PC471_003765"
     CDS             2718..>3211
                     /locus_tag="PC471_003765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage scaffolding protein"
                     /protein_id="HDF2790841.1"
                     /translation="MDWLKELLEGIKIEDNKIDVVSLQKSIEKKIKETTVTQEEYTNI
                     ETQLNTANETIKKFEGGMTKEDVENLKTTYETDKKTLEETYKKEIEEKDFNYWLGDAF
                     KSVKCRDEIALKAHLDIEALRNSKDRQKAFEEQINPLKQDKDYLFNATLEGEEPKIDT
                     ITPG"
BASE COUNT         1378 a          287 c          594 g          952 t
ORIGIN      
        1 gaaaagaata taattatctt tggtcaagga gttaatccag aatctcaaaa tacaggtgac
       61 aaatcgggtg tggcacttaa atttttatat tcactacttg atttaaaatg ttctaagact
      121 gaaaagaagt ttaaaaaagc aattagagag cttttatggt ttgtgtgtga gtatttaaag
      181 ataagtggta gtaagagcta tgattataaa acagttcaaa tcacttttaa tcactctatg
      241 ataataaatg aagctgaaaa gatagatatg gcagctaaat caactggaat tatatcagat
      301 gaaactattg ttagtaatca cccatgggtt gaagatgtta atgacgaact tgatagactt
      361 aaaaaacaga aagaagaaag tattaaagaa tatgaagatg cttttccaac taaaaagaaa
      421 actgaagaag gtgagccttt agatgaataa tataaattac tggataaaga catttaatca
      481 gttagaagaa gatattcata acaaaggtga taactttaac aaggaactag aaaaacaata
      541 tgacataata ctttacaata tccttagaga aataaataat tggtatatga aatttgctaa
      601 ggataataaa ataagcatgc aagaagctga aaagttatta aattcaaagg aattaactga
      661 acttaaatta tcattagaag aatatataaa atatggagaa gaaaatgtaa ttagtcaaaa
      721 gtggatgaaa gaactagaaa gtgcagtcaa aagagttcgt ataagtagac tagaagcatt
      781 aaaattaaag ataaggcatc aagttgaggt attatactca aaagaatatg aggatgtaaa
      841 tacacttata acaaatacct ataaagatag ttactatcat actgcttttg aagctcaaaa
      901 aggtattgga attggattaa tgatagccat gcttagcatg aataatatta acaaaatagt
      961 tagtagtcct tggactactg atggaataac atttggtaat agaatttgga ataagcatag
     1021 accaaggtta ataagcgaac ttaacaaagg attaaaacaa actttaatta atggagtatc
     1081 tcctgaaaac ttaacaaata aaattaataa aaattttaat acaagtaaag aagaagctaa
     1141 gttattagta aaatctgagc ttgccttttt tagttcatta agtcaaaggg attgttttaa
     1201 tagtctaggt gttgagaaat atgaaatagt aagtgcatca gatagtaggg tttgtgagaa
     1261 aaggtgtagc cctcttgatg gtaaagtaat tgagatgaaa tattatgaaa taggaattac
     1321 agctccacca tttcatccta gatgtagatg tgtagtagta ccatattttg atggtgagga
     1381 aagttatagg gcagcaagag aagaagatag tgagacttat tatgttccag catcaataaa
     1441 gtataatgag tggtattcga agtatgtaaa agatagtttc tcaagtggag caataagcag
     1501 aaaaagacta aaggacctag aaatatatct aagagaagaa aaacatgcta atttatatta
     1561 tgaagaaata agaaaaagaa ccagtgatat taaagctata gctaaaaata caggctatag
     1621 tgaaaaagta atacaagata taaaaagcca tgtatttatg aataaatata atttatatgg
     1681 cggatataaa agttttgacc ctagttatga tatggctatc tcttggcaaa gattaattga
     1741 gggaaaagat ataagaaaat gtgatataat attattaaaa catgagagat tagaaaagtt
     1801 tcttatggat agatataatt atgattataa gaaagctcat ggtttagttg tgagaaaata
     1861 tgattatgaa aaagctttga gggagggata aacatggtac ttataaagaa aaattatata
     1921 aaagatgata aagtatctta tttttatcaa ccagaagaag atggagagtt tggagaagtt
     1981 atactttata ctaaagataa atcttttgat atagtaaaaa tagcagaaag agacagtgaa
     2041 gggaatcatt tttatatgtc tcatgtttac tgtatgatac gtaaatttat agaagaaaat
     2101 aattatccag atgagaaaac atcttattgg tattagaaag cacttgctaa ataatatatt
     2161 agtaggtgct tttattatgt aaaaatttat tgagagggtg attttaatgt gtatattaac
     2221 tcaagttata gttgtagttt gtgtagtaca aatcgttatt aattgtattg ctaatcttaa
     2281 tgtaggtatt ctttgtaata aattaaaaaa agaaaataaa gctaatatag ataaagtttc
     2341 tgatgaaatt ttaaagagag taagagaaga attaaataaa tcattagata aaagtctata
     2401 agaagttttt tattaaataa aaatttaagg aggcttattt tattgaaaga gtatataatt
     2461 tggtttaaga gtggaaattg tgtatctgga ataacagacg aatatgttgc tgataagcta
     2521 atgaaagatt ttatgggagc tgactcagat tgtaggaact tgaaaggata tttagatgaa
     2581 gatggaacaa caataataga tttatcgcaa atagaagcta tgtcaataaa taattgtaat
     2641 gagaataata atattggttt tagtaagtcc tagatagggc ttttttatta tgtaaaaaaa
     2701 gaaaggagac atttaaaatg gattggttaa aagaattgct agaaggaata aaaatagaag
     2761 ataacaagat tgatgtagtt tctcttcaaa aatctataga aaagaaaata aaagagacta
     2821 cagttactca agaagagtat acaaatattg aaacacaact taatacagct aatgaaacta
     2881 ttaaaaagtt tgaaggaggt atgacaaaag aagatgtaga gaatctaaaa acaacttatg
     2941 aaactgataa gaaaactttg gaagaaacct acaaaaaaga aattgaagaa aaagatttta
     3001 attactggtt aggtgatgct tttaaatctg ttaaatgtag agatgaaata gcattaaaag
     3061 ctcatttaga tatagaagca ctaagaaata gtaaagacag acaaaaagct tttgaagagc
     3121 aaataaatcc tttgaaacag gataaagatt atttgtttaa tgcaacacta gaaggtgaag
     3181 aacctaaaat agatactata acaccagggc a
//
LOCUS       DAMJYR010000235         3175 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.240, whole genome shotgun sequence.
ACCESSION   DAMJYR010000235 DAMJYR010000000
VERSION     DAMJYR010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3175)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3175)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3175
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.240"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(<1..615)
                     /locus_tag="PC471_003766"
     CDS             complement(<1..615)
                     /locus_tag="PC471_003766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 transporter permease"
                     /protein_id="HDF2790842.1"
                     /translation="MFNLIRKDLIIGISSDGIRNLKYILLFFVFYFFLNSISYYTVSI
                     VVSYLIFINTFECDYENDSRIFIRSMPVSIEDIVYSKYLLGVGLIISVTIIVSLLSKL
                     TSLVFFRNMVLNDVFFSVNIFLAILTILLPLIFKFGYGKMKVCGFIVSILIYFVYGSL
                     LKMISMIVYQVKHFNYSKVGGVYLSNYVTDIANTKYINLYSMTFL"
     gene            complement(615..1475)
                     /locus_tag="PC471_003767"
     CDS             complement(615..1475)
                     /locus_tag="PC471_003767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2790843.1"
                     /translation="MFSIRNITKKLGKFKLNNINLELKEGDIVGIIGPNGSGKTTLIK
                     IIMGIIDADEGEIELCNETIENSPISFKNNIGFVYDSLQFYPHLKVKEFRKIVSLFYK
                     NFDRERFDEYLNKFDIEENMHIENLSKGQSEKLMLSSALSHNAKLLILDEPTAGIDPI
                     VRTEIMQYLQDFVKNGSSSVIISTHNTDNLIKIADYLVFINRGNQIFTVKKELIEQEY
                     KIIRANKAELEAIKESIVGVKEYKYYNEALVKVGDSLKVKSLLIEIDKHKVKNPTIEE
                     LMYYYVNEVR"
     gene            1610..2410
                     /locus_tag="PC471_003768"
     CDS             1610..2410
                     /locus_tag="PC471_003768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HDF2790844.1"
                     /translation="MKRIITINTNQLNKSLETKELLTRKLINAGFEVYSDIYPDTELI
                     ISIGGDGSFLRTVRDFDFPEIPIMGINTGHLGFFPDILPDKIDSFIEAYTKKDYIIQE
                     MSLLNAEVYTTTSGSNMLAVNEVVIRGDKSRTIHLNLSLDNKHIQNFSGDGMIISTST
                     GSTAYNYSAGGSIVDINLELMQITPLHPINTNAYRCFTSSIICSNESVIKIAPEYRFE
                     DSVLIVVDGVEHRFRQIENIKVSISDAKIKLLRMSNYEFWHRVSEKFL"
     gene            2435..3004
                     /gene="yfcE"
                     /locus_tag="PC471_003769"
     CDS             2435..3004
                     /gene="yfcE"
                     /locus_tag="PC471_003769"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HDF2790845.1"
                     /translation="MKIGIMSDTHGSLLYFEKALNVLSDCDVLLHGGDVLYHGPRNDI
                     PEGYNPKKFIETLNKLENIVIVKGNCDADVDQMVIEHPIQSPYVMSQFGEIRIILNHG
                     YIESEEEIIDKAKKMGGDILVLGHTHVKKLYMDDNLIVINPGSTSIPKDGSHSVAIID
                     IIKTDDEDELELDINLIDINTGNIININD"
BASE COUNT         1190 a          466 c          383 g         1136 t
ORIGIN      
        1 taaaaatgtc atactatata aatttatata cttagtatta gctatatcag ttacataatt
       61 tgaaagatat actcctccaa ctttactata attaaaatgc tttacttgat atacaatcat
      121 actaatcatc ttcaaaagac ttccatatac aaagtaaatt aaaatactta ctataaaacc
      181 acaaactttc atttttccat atccaaactt aaaaattaaa ggcagtaaaa tcgtcaaaat
      241 tgctaaaaat atatttacag aaaaaaatac atcatttaaa accatatttc taaagaacac
      301 tagtgatgtc aatttactta gtaaggacac aattattgtt acagaaatta ttaatcctac
      361 acctaataaa tattttgagt aaactatatc ttctatacta actggcatac tccttataaa
      421 aatccttgaa tcattttcat aatcacactc aaatgtattt ataaatatca aataactaac
      481 aacaatactt actgtataat atgaaattga attaaggaaa aaataaaaca caaaaaatag
      541 caatatgtac tttaaattac ggataccatc tgaactaatc cctattatta aatcttttct
      601 tattaaattg aacatcatct cacctcattt acataataat acattagttc ttctatagta
      661 ggatttttga ccttatgctt atcaatttct atcaataggc tttttacttt caagctatct
      721 ccaactttaa caagtgcctc attataatat ttatactctt tcacaccaac tatgctttct
      781 ttaattgctt ctaactctgc tttatttgcc ctaattatct tatattcttg ttctattaat
      841 tctttcttaa cagtaaaaat ctgatttcct ctatttataa aaacaagata atcagcaatt
      901 ttaattaaat tatcagtatt atgagtagaa attattacac tactacttcc atttttcaca
      961 aaatcttgta agtactgcat tatttcagtt cttacaattg ggtctatccc agcagttggt
     1021 tcatccaaaa tcaataactt agcattgtga ctaagtgcac ttgataacat aagcttttca
     1081 gattgacctt tagataaatt ttcaatatgc atattttctt ctatatcaaa cttattaagg
     1141 tattcatcaa acctttctct atcaaaattc ttataaaata aagaaactat ttttctaaat
     1201 tccttaacct ttaaatgcgg ataaaattgt aaagaatcat atacaaaacc aatattgttt
     1261 ttaaagctta ttggagaatt ttcaatagtt tcattacaaa gttcaatttc accttcatct
     1321 gcatcaataa tccccattat aatttttatt aaagtcgttt ttcccgaacc atttggtcct
     1381 ataatcccaa caatatcccc ttcctttaac tctaagttta tattatttaa cttgaatttt
     1441 cctaattttt ttgtgatatt ccttatgcta aacacatctt cacctcttat gcattttttt
     1501 atacccacct atacatattt taacacattc actacaaagg tcatttataa atttataaat
     1561 taagaattat aaaactatgt tataatacaa agtatgaaga ggtgaatata tgaaaagaat
     1621 tataactata aatacaaacc aattaaataa gtcccttgag actaaagaac tcttaactag
     1681 aaaacttatc aatgctggct ttgaagtgta ttcagatatt tatccagata cagagcttat
     1741 tatatcaatt ggtggtgatg gctctttttt aagaacagtt agagatttcg attttcctga
     1801 aattcccata atgggaataa atacaggtca tctggggttt tttcctgata ttttaccaga
     1861 taagattgat agctttattg aagcttatac aaaaaaagat tatattattc aagaaatgtc
     1921 acttcttaat gcggaagtat atacaactac tagtggttct aatatgcttg ctgtaaatga
     1981 ggttgttata agaggagaca agtctaggac tatacaccta aatttaagcc ttgataataa
     2041 acatatacaa aattttagtg gagatggtat gattatttct acttccacag gttcaacagc
     2101 ctataactat tctgctggag gtagtattgt tgatataaat cttgaactta tgcagattac
     2161 acctttacat ccgataaata ccaatgcata taggtgcttt acatcaagta taatatgttc
     2221 aaatgaatct gttataaaga ttgcaccaga atatagattt gaagattctg tattgattgt
     2281 cgttgatgga gttgagcaca gatttagaca aatcgaaaat ataaaagtta gtatatctga
     2341 tgcaaaaata aaattactta gaatgtcaaa ctatgagttt tggcatagag tatctgaaaa
     2401 atttttatag actgtactta ggaggatttt taatatgaaa ataggaataa tgagtgatac
     2461 tcatggtagc ttactttact ttgaaaaagc gttaaatgta ttatctgatt gtgatgtttt
     2521 attacatggt ggagatgtct tatatcatgg acctagaaat gatattccag aaggatataa
     2581 tcctaaaaaa tttattgaga cattaaataa attagaaaac attgtgattg taaaagggaa
     2641 ctgtgatgca gatgttgacc agatggttat agaacatcct attcagagtc cttatgtcat
     2701 gagccaattt ggtgaaatta gaattatatt gaatcatggt tatatagaat cagaagaaga
     2761 aattattgac aaagcaaaga aaatgggtgg tgacatatta gttttaggac atactcatgt
     2821 aaaaaagcta tacatggatg ataatttaat agtaataaat ccaggaagta cttctatccc
     2881 aaaagatgga agccattctg tcgcaattat agatatcata aaaactgatg atgaggatga
     2941 gcttgaactt gatataaact taattgacat caatactgga aatataataa atatcaatga
     3001 ttagtaaatt tttaataaaa ataaaataag ctttaattta ttaaactcat aataaaaaca
     3061 agtaaaccat tttatttcta tctcaattag aagtttaagt acaatacctt aaacttctaa
     3121 ttaaatagaa tctggtttac ttgttttatt atatgctata ttgaaatata actta
//
LOCUS       DAMJYR010000236         3141 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.241, whole genome shotgun sequence.
ACCESSION   DAMJYR010000236 DAMJYR010000000
VERSION     DAMJYR010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3141)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3141)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3141
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.241"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     misc_binding    114..376
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            452..3118
                     /locus_tag="PC471_003770"
     CDS             452..3118
                     /locus_tag="PC471_003770"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HDF2790846.1"
                     /translation="MENTNLSKTYNPKDFEARLYKKWMDEGYFKSKPNPDKKPFTIMM
                     PPPNITGQLHMGHALDHTLQDILIRWKRMDGYEAFWLPGTDHASIATEVKVVERIKKQ
                     EGKTKYEIGREEFLKRAWEWKDEFGGKISNQLKQLGDSCDWDKERFTMDEGCNEAVIE
                     FFVSLYEKGHIYRGNRIINWCPDCKTTLSDAEVEHEEHDGNFYHIKYPLKDSEDFLEI
                     ATTRPETMIGDTGIAVNPEDDRYKHLIGKTAILPLVGRELPIVADSYVDLEFGTGAVK
                     MTPAHDPNDFEVGLRHNLEQLNTMNEDGTMNEVCGKYEGMDRFECRKAIVADLKEQGY
                     LIKIKEHNHNVGTCYRCHTVVEPRLSEQWFVKMEELAKPAIDILKKGELEFVPDKFDK
                     TYLQWLENIRDWCISRQLWWGHQIPAYYCQECGEIVVARKMPDKCPKCGSTHFKQDED
                     ALDTWFSSALWPFSTLGWPNKTEALDYYYPTSVLVTGYDIIFFWVVRMAFAGMFCMNE
                     KPFDHVLVHGLVRDSQGRKMSKSLGNGIDPLEIIEQYGADALRFTLTTGNSPGNDMRF
                     YMERVEFARNFANKLWNASRFVFMNIDEDIIKNMTRESVKEDLTLADKWIISRANNIV
                     KEATNNMDKFDLGIALQKIYDFTWSEYCDWYIEMVKPRLYGEDANAKSAALYTLTYVL
                     EKILKLLHPYMPFITEEIYTHLPTVEGCIIVSEWPKYNEEDNMAEEEDMMNLLMEGIR
                     SIRNVRAEMNVPPSKKAKLIIIPSEEKIEAIELGKDYFITLASASNVEIAKDKSNVPE
                     DAVGVVIDGVEIFIPLNELVDFEKEIERLSKEKKKLEGEIKRVNGKLANQGFLAKAPE
                     SLIEEEKAKKEKFEEMIKSVEERLTNLESKIK"
BASE COUNT         1277 a          350 c          613 g          901 t
ORIGIN      
        1 ttaaaatata acaactatat aaaagagaat agacataatt aagaaaattc agaaaaaata
       61 aaataaaaat aattgacaaa gagaataata tttgtatata ataatatgta atattaaaga
      121 ctgagaagaa gaggagtaag taaatactac taaaagagag gaaaattcac tggctgtaag
      181 attttctaag tataagattt actgaatgta gctttggagt ttctaaactg aactataagt
      241 aagtttagac ggttagaacc gttaaattct ttgagagtca aagttgataa aataacatta
      301 accaataatt taatgatatg ttaatgtatt ttaagctttg gaattaaggt ggtaacgcga
      361 gcttttcgtc ctttttaaag aggatgaaga gctctttttt atttctaaag atatttaaaa
      421 aatttattta taaaatacag gaggcaggac aatggaaaat acgaatttat caaaaactta
      481 taacccaaaa gattttgagg ctaggctata taaaaaatgg atggatgaag ggtattttaa
      541 atctaaacca aatccagata aaaaaccatt tacaataatg atgccaccac caaatataac
      601 agggcaattg catatgggac acgctcttga ccatacactt caagatatat taatcagatg
      661 gaaacgtatg gatggatatg aagctttttg gcttccagga actgaccatg cttctatagc
      721 aacagaagtt aaagttgttg aaagaataaa aaaacaagaa ggcaagacaa agtatgaaat
      781 aggtagagaa gaatttttaa agagagcctg ggaatggaaa gatgaatttg gaggaaaaat
      841 atctaatcag cttaaacaac taggtgattc ttgtgattgg gataaagaaa gatttactat
      901 ggatgaaggg tgtaatgaag cagttataga attttttgta agcctatatg aaaaaggtca
      961 catatacaga ggaaatagaa taataaactg gtgcccagat tgcaaaacta ctttatcaga
     1021 tgcagaagtt gaacatgaag aacacgacgg aaacttttat catataaagt acccactaaa
     1081 agatagtgaa gactttttag aaatagctac aactagacct gagacaatga taggagatac
     1141 tggtatagca gtaaatccag aagatgatag atataaacat ttaataggaa aaacagcaat
     1201 acttccatta gtaggaagag aacttccaat agttgcagat agttatgttg atttagagtt
     1261 tggaactggt gcagttaaga tgactccagc acatgaccct aatgattttg aagtaggatt
     1321 aagacataat cttgagcagt taaatactat gaatgaagat ggaactatga atgaagtttg
     1381 tggaaaatat gaaggtatgg atagatttga gtgtagaaaa gctatcgtag cagacttaaa
     1441 agaacaaggt tacctaatta agataaaaga gcataatcat aatgtaggaa cttgttatag
     1501 atgccataca gtagttgagc ctagattatc agagcaatgg tttgttaaga tggaagaact
     1561 tgcaaaacca gctatagata ttcttaaaaa aggagaatta gaatttgttc cagataaatt
     1621 tgataaaact tatttacaat ggctagaaaa tataagagat tggtgtatat caagacaatt
     1681 atggtggggt catcaaatac cagcttatta ttgtcaagag tgtggagaaa tagtagttgc
     1741 tagaaaaatg ccagataagt gtcctaaatg tggaagcact cattttaaac aagatgaaga
     1801 tgccttagat acttggttca gttctgcatt atggcctttc tctactctag gatggccaaa
     1861 taagactgaa gctttagatt attattatcc aacaagtgta cttgttacag gatacgatat
     1921 aatattcttc tgggtagtta gaatggcatt tgcaggtatg ttctgtatga atgaaaagcc
     1981 atttgaccat gttttggttc atggattagt aagagattct caaggtagaa aaatgagtaa
     2041 atctttagga aatggtatcg acccattgga gataatcgag caatatggag ctgatgccct
     2101 aagatttaca ttaacaactg gaaattcccc aggaaatgat atgagattct atatggaaag
     2161 agtagaattt gctagaaact ttgcaaataa gttgtggaat gcatcaagat ttgtatttat
     2221 gaatattgat gaagatataa ttaaaaacat gactagagaa agtgttaaag aggacttaac
     2281 tctagctgat aaatggataa tatcaagagc aaataatatt gtaaaagaag caactaataa
     2341 catggataag tttgatttag gaatagcact tcaaaagata tacgatttta cttggtcaga
     2401 atactgtgac tggtatattg aaatggttaa acctagatta tatggagaag atgcaaatgc
     2461 taaatctgca gcattatata cattaactta tgttcttgaa aaaatattaa aattattaca
     2521 tccatacatg ccatttataa cagaggaaat atatactcat cttcctactg ttgaaggatg
     2581 tataatagta tctgaatggc caaaatataa tgaagaagat aacatggctg aagaagaaga
     2641 tatgatgaac ttattgatgg aaggtataag aagtataaga aatgtgagag cagaaatgaa
     2701 tgtaccacct tctaagaaag ctaaattgat aataatccca agtgaagaaa aaatagaagc
     2761 tattgagctt ggtaaagact attttataac tttagcatct gcttcaaatg tagagatagc
     2821 aaaagataaa tctaatgttc cagaagatgc agttggagta gttattgatg gtgttgaaat
     2881 atttatacct cttaatgaat tagttgattt tgaaaaagaa attgagagat tatctaaaga
     2941 aaagaaaaag ttagaaggcg aaataaagag agttaatgga aagcttgcaa atcaaggttt
     3001 cttagcaaaa gctcctgaaa gtttaattga agaagaaaaa gcaaaaaaag aaaaatttga
     3061 agagatgatt aaatctgttg aggaaagatt aacaaattta gaatctaaaa taaaataaat
     3121 ataagaatta gtaaaaacat a
//
LOCUS       DAMJYR010000237         3091 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.242, whole genome shotgun sequence.
ACCESSION   DAMJYR010000237 DAMJYR010000000
VERSION     DAMJYR010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3091)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3091)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3091
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.242"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     repeat_region   9..235
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=76..104
                     /rpt_unit_seq="atttacattccatatagttaatataaaac"
     gene            complement(894..2987)
                     /locus_tag="PC471_003771"
     CDS             complement(894..2987)
                     /locus_tag="PC471_003771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HDF2790847.1"
                     /translation="MNKHNFEVILNQLQINIYVTNIHTNEIIFMNKKMKEEYNILDPE
                     GKVCWQVLYPEKNSTCSFCKVLELLKNDKKGVLIKWYEKCNKLNRVFENYDSLITWQD
                     GTVVHMHQSIDIANSTSLNKPIKINEFHEISNNKEEKGVFNFSRDNFDYNSTLLYDAL
                     IRGTDEYIYICNMKTGVFRYSPSQVELFDLPGEIVKNPLVYWKKIVHPEDWNRFYKSN
                     TEIGKNQMDYHTVEFRAKNRSGEYIWLRCRGQLMRDEFGEPSIFAGIMTQLGKQNKID
                     SLTQLLNYHEFMSVFEDKISNPMIEKLCIVLLDIDDFKNVNEMYDRDFGDNIIKTLAQ
                     SVQSILPDNAELYKLDGDEMGILVDNVEENEILTLYNQIQNMIIHLQLWRKYGLNITI
                     SAGCVIYPKHGDTVKELYKCASYSLQYAKEHGKNRLVFFSQEILKNKMYSLEMMRDLK
                     ASINDDFRGFSLRFQPQVDTESHKIIGVEVLLRWTNDKCKAISPLEFIPILEENDMIN
                     IVGAWVLRMALRTFRKWIDYYPFFKVSVNVSAVQILEDTFIEDIVKIIDDENFPYQNL
                     VLELTESHTVQNMSILQFKFKALQDLGIYIAMDDFGTGYSSLEVLKFSPIDIVKIDRV
                     FVKDILKSKFDATFIHFIVAICHDVGIKVCLEGVETQEEYDLVKQIKPDYIQGYLFGK
                     PQTATEIFDLLKLDN"
BASE COUNT         1073 a          509 c          291 g         1218 t
ORIGIN      
        1 ttatgaaaac ttacattctg tatagttaat ataaaacata agtaatataa atttatgtaa
       61 agcaacaaaa gatagattta cattccatat agttaatata aaaccttaga gaaagcgaaa
      121 acgtaccatt aaaatcaaat atttacattc catatagtta atataaaacg ttgtaatcat
      181 agttccaatt agccacttta acatgcattt acattccata tagttaatat aaaacaccaa
      241 aataaactta gtattttcaa cacttacacg tatacaactc tcctaaattt gcagtgaacg
      301 agcaatagtg caattgataa cgcttatcat gcactctcaa cgccttatat ttcaactgtt
      361 acgccaaatt ttttcacaaa tatcgctcac tgcaaaattc ctataatttt attatatcat
      421 aaatatatta cttttgaata ttgataccaa tttataccat aataaaagca aaaaaacgta
      481 atctatctac attagattta aattctataa cttaaagttt tttacataat atttatgctt
      541 gtttaatcaa tctaatatat cattcttgta tattgctttt aatagtcttt tttcttaaat
      601 aaatatctat ctgaacaagc atagtttaat taaaaacttt tgtattccct taaaactgta
      661 tctacctctt attattttat ttatgatata ttatttttaa tctaaaaata gatatctatt
      721 tttattatgg ttatcccttt ttaaaatact ttaatcatat actattccca atttaacatc
      781 cattgaaatg ctattttctt atattgcaat ttataattga tatatcttca atataataat
      841 ttaatgtatt tttgatttta aagcttttca gtgatattat tgataaatta gaattaatta
      901 tctagcttta aaaggtcaaa gatttctgtt gctgtctgcg gttttccaaa taaatatcct
      961 tgtatgtaat ctggctttat ttgtttaact aaatcatact cttcttgtgt ttctacacct
     1021 tctaaacata cttttattcc tacatcatga caaattgcaa caataaaatg tataaatgta
     1081 gcatcaaact tactctttaa tatatcttta acaaatactc tatcaatttt cactatatct
     1141 attggagaaa attttaaaac ttctagagac gagtatccag taccaaaatc atccatagca
     1201 atataaattc ctaaatcttg taaagcctta aatttaaact gtaaaatact catattttgt
     1261 actgtatgac tttctgtcaa ttctaaaaca agattttgat aaggaaaatt ttcatcatct
     1321 ataattttta ctatatcttc aataaatgtg tcttctaata tttgaacagc tgaaacgttt
     1381 acactcactt taaaaaaagg atagtaatca atccatttcc taaaagtacg taatgccatt
     1441 cgaagcaccc aagcccctac aatatttatc atatcatttt cttctaatat tggaataaat
     1501 tccaaaggag aaattgcttt gcatttgtca ttagtccacc ttaaaagaac ttctacacct
     1561 attattttat gactttctgt atctacttgt ggctggaatc gaagactaaa ccccctaaaa
     1621 tcatcattaa ttgatgcctt taaatcccgc atcatttcta atgaatacat tttatttttt
     1681 aatatttctt gggagaaaaa aacaagcctg tttttaccat gctctttcgc atattgtaaa
     1741 gagtaactag cacacttata aagttctttt actgtatctc catgttttgg atatattaca
     1801 caaccagcag aaattgttat atttaatcca tattttctcc atagttgaag gtgtataatc
     1861 atattctgta tttgattata tagagttaga atttcatttt cttcaacatt atcaacaaga
     1921 atgcccattt cgtccccatc aagtttataa agttcagcat tatctggtaa aatagattgt
     1981 acagactgtg ctagtgtttt tattatatta tctccaaaat ccctatcata catctcatta
     2041 acatttttaa aatcgtcaat atctaataat actatgcata atttttcaat cataggattt
     2101 gaaattttat cttcaaatac actcataaat tcatgataat tcagtaactg tgtcaatgaa
     2161 tcaatcttat tttgtttacc taattgcgtc attattcctg caaaaatact tggctctcca
     2221 aattcatcac gcattagttg acctctacat cttaaccata tatattctcc actacgattt
     2281 ttagccctaa attccactgt atgataatcc atctggtttt tacctatttc agtgtttgac
     2341 ttataaaaac gattccagtc ttctggatgt acaatttttt tccagtaaac aagcggattt
     2401 ttaacaattt ctcctggtaa gtcaaataat tcaacttgtg atggtgaata tcgaaatacg
     2461 cctgttttca tattgcaaat gtatatatat tcatctgtac ctctaatcaa agcatcgtaa
     2521 agcaatgtag aattataatc aaaattgtct cttgaaaaat taaaaactcc tttttcttct
     2581 ttattatttg atatttcatg aaattcatta atcttaattg gtttatttaa gcttgttgag
     2641 tttgcaatat ctattgattg atgcatatga acaactgtgc catcttgcca tgtaattaaa
     2701 ctatcataat tttcaaaaac acgatttaac ttattacatt tttcatacca ctttattaat
     2761 actccttttt tatcattctt taataattct aaaactttac aaaaactaca agtagaattt
     2821 ttttcaggat ataatacttg ccaacaaact tttccttctg gatctaaaat attatattct
     2881 tctttcattt ttttgttcat aaaaataatt tcattagtat gaatatttgt aacatatata
     2941 ttaatctgta gttgatttaa tataacttca aaattatgtt tgttcatata cttatctcct
     3001 ccatagttac tgctctttat aacaacattg ttaaaattat tctttttaga agatttactt
     3061 ataacttata catatacact aaatacctta a
//
LOCUS       DAMJYR010000238         3010 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.243, whole genome shotgun sequence.
ACCESSION   DAMJYR010000238 DAMJYR010000000
VERSION     DAMJYR010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3010)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3010)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3010
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.243"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            385..1662
                     /gene="hflX"
                     /locus_tag="PC471_003772"
     CDS             385..1662
                     /gene="hflX"
                     /locus_tag="PC471_003772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HDF2790848.1"
                     /translation="MDMAKKGITVGLNINNKSEDFNELMIELENLCSACDIDVVGSIT
                     QNAKQVNRAFYIGTGKVEEILNLIKKENIEIVIFYNELSTSQLKNLEEKLNCEIIDRT
                     ALILDIFAQRAKTREAKLQVEVASLKYMLPRLIGSNENLGRQSGGVGTKNRGSGEKKL
                     ELDRRRIEEKITSLNRELDDLKFQRETQRSMRRKSNLPNVALVGYTNAGKSSIMNKLV
                     DIFKNSEEKKVFEKNMLFATLETSVRNIVLANNKEFLLSDTVGFVSNLPHDLVKAFRS
                     TLEEACEADVLLHVIDISNPSYKSHIKVTEDTLKQIGADGIPMIHVYNKIDLIDVEVL
                     DRILDSIDKEGIFVSVKKDINIDKMIKCICDSIFKDYVRCKFLIPYDKGHVVSYFNEN
                     TSIINTEYREDGAILDVECSNIEYNKYKKYALE"
     gene            1895..2392
                     /locus_tag="PC471_003773"
     CDS             1895..2392
                     /locus_tag="PC471_003773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDF2790849.1"
                     /translation="MDAESLMKKYMVDDDSRKQAIFASIFVMQNKLQTTCDKLDPELT
                     MKQWLLLAIADSVDETLTLTKLGELMGCSRQNVKKLAIALEKKGFIQIEQNEKDSRAI
                     CLVTKDRVQEYSKKVRGMQEKVLQLLFEDFTEKEVEQLFFGIMKLSIGIENVKNYVEN
                     NDVGE"
     gene            2442..2966
                     /locus_tag="PC471_003774"
     CDS             2442..2966
                     /locus_tag="PC471_003774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HDF2790850.1"
                     /translation="MKNVVVIYKSKYGSTKKYAKWLSELLSCDIFETKNINIEKVMKY
                     DTIILGGGLYACNIAGISFLKKNYKELQKKNLVVFGVGAAPYDDEVIKKLREVNFKDE
                     LNKIPCFYCRGAWDETKMSFIDYTLCSFFKKSIAKKERQGLKPAEAPLLSGEAFDWCN
                     KENLTPILDYIREL"
BASE COUNT         1247 a          265 c          531 g          967 t
ORIGIN      
        1 ttattaatca tgcaaataac tacttaaatt taagaatagg gttaaatata aaatgataat
       61 tacatataag ttaattaaac taatgtattt ttaatatata tttagaattt taatccttct
      121 tatgatataa gtagcttgtt ttgtattact tagatttgta aggagttttt ttattgaaaa
      181 aactgcataa tatatttggg aggaggtgta agaaagtgat tttttatata agattgagaa
      241 aataatgcac gattatggtt attataatag taaataagaa gtgtcattta aggataagat
      301 aaaaagcaaa attcttgatg aatttaatta tagagccata atctcgtgct atagattaaa
      361 taaaaaatag cagataagga gaatatggat atggcgaaaa aaggaataac agtaggatta
      421 aacataaata ataaatctga ggactttaat gaactaatga ttgaacttga aaacttatgt
      481 tcagcatgtg atattgatgt tgtagggagt ataactcaaa atgcaaagca ggttaataga
      541 gcattttaca ttgggacagg taaagttgag gaaatattaa acttaataaa gaaagaaaat
      601 atagagatag ttatatttta caatgaactt tctacatcac aactaaaaaa tcttgaagaa
      661 aagcttaatt gtgaaattat agatagaacg gcattgatac ttgatatatt tgcacaaagg
      721 gcaaaaacta gagaagctaa acttcaagta gaagtagcaa gtttaaaata tatgttacca
      781 agacttatag gttcaaatga aaatttagga cgtcaaagtg gtggagttgg tactaaaaat
      841 agaggttctg gtgagaaaaa attagaactt gaccgtagaa gaatagaaga aaagataaca
      901 tctttaaata gggagttgga tgatttaaaa ttccaaagag aaactcaaag aagtatgaga
      961 agaaaatcaa atcttccaaa tgtagctcta gtcgggtata caaatgctgg taaatcttca
     1021 ataatgaata aactagtaga tatatttaaa aactctgaag aaaagaaagt gtttgagaaa
     1081 aacatgttat ttgctacact tgaaacttct gtcagaaata tagttcttgc aaataacaaa
     1141 gaatttttat tatctgatac tgttggtttt gtaagtaatc taccacatga cttagtaaaa
     1201 gcatttaggt caacattaga agaagcatgt gaggcagacg tactgttaca tgttattgat
     1261 atttcaaatc caagctataa aagtcatatt aaagtgacag aagatacttt aaaacaaata
     1321 ggtgcagatg gtatacctat gatacatgtg tataataaaa ttgatttaat agatgtagaa
     1381 gtattggata gaattttaga tagtatcgat aaagaaggta tatttgtttc agttaaaaaa
     1441 gatataaata tagataaaat gataaaatgt atttgtgata gcatatttaa agattatgta
     1501 agatgtaagt tcttaattcc atatgataaa ggtcatgtag tttcttattt taacgaaaat
     1561 acgagcatta taaatacaga atacagagaa gatggagcta ttttagatgt agaatgtagc
     1621 aatatagaat ataataagta taaaaagtat gctttagagt aaattttgaa aaacttatta
     1681 tagcctaaaa tatatttaat ttataattta ttataaattg attgtaaata tgattttatg
     1741 aaacggtata gccataatgc tatgccgttt tgccgtttat aataaaaata aatttattga
     1801 cataatagta actaattact ataatataaa gtaattagtt actatttaaa attatcaaaa
     1861 aattaaaatc taagataaag agaagtgtat aataatggat gcagaaagtt taatgaaaaa
     1921 atatatggta gatgatgata gtagaaaaca agctatattt gcaagtatat ttgtaatgca
     1981 aaataaactt caaactacat gtgataaatt agacccagaa ttaacaatga aacaatggtt
     2041 acttttagct attgcggact ctgtagatga aactcttaca ttaaccaaac ttggagaatt
     2101 aatggggtgc tcaagacaaa atgtaaaaaa actagctatt gctttagaaa agaaaggttt
     2161 tatacaaatt gagcagaatg aaaaagattc aagagctatc tgtcttgtga caaaagatag
     2221 agtgcaagag tattctaaaa aagtaagagg tatgcaggaa aaagtattac agcttttatt
     2281 tgaagatttt acagagaaag aagtggaaca gttatttttt ggaattatga aattaagtat
     2341 aggaattgaa aatgtgaaaa attatgtaga aaataatgac gttggagaat aagcaagtat
     2401 caaatcaact ttgtatgaca tcagagaggg ggataattta tatgaaaaat gtagtagtga
     2461 tttataagag taagtatggt tcaacaaaaa aatatgctaa atggctttca gaattattgt
     2521 cttgtgatat ttttgaaact aagaatatta atatagaaaa ggttatgaag tatgatacga
     2581 ttattttagg aggaggtctt tatgcttgta atatagcggg aatttctttc ttaaaaaaaa
     2641 attataaaga attgcagaaa aaaaatttag tagtatttgg cgtaggagct gctccatatg
     2701 atgatgaagt aataaagaaa ttaagagaag taaattttaa ggatgaatta aacaaaattc
     2761 cttgctttta ttgtcgtggg gcatgggatg agacaaaaat gtcttttata gattatactc
     2821 tttgtagttt ttttaaaaaa tctatagcta aaaaagaacg tcaaggtctt aaaccagcag
     2881 aagcaccttt attgtctgga gaagcctttg attggtgtaa taaagaaaat ttaactccaa
     2941 tacttgatta tataagagaa ctttaagagg tatatatttg taattattta ttatctaaag
     3001 ttagatttca
//
LOCUS       DAMJYR010000239         3007 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.244, whole genome shotgun sequence.
ACCESSION   DAMJYR010000239 DAMJYR010000000
VERSION     DAMJYR010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3007)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3007)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3007
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.244"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            99..605
                     /locus_tag="PC471_003775"
     CDS             99..605
                     /locus_tag="PC471_003775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HDF2790851.1"
                     /translation="MKEYVVVDLETTGLDPYKGCEIIEIGITEIKNEQIVKNYSRLIK
                     PKGIISSFITELTHISNEMVENEEPLELVLPRFRKYIGDRTIIAHNAKFDLKFLNYYL
                     RMLDLEPINNYICTVELLKKCKSYKGKNKKLETACAYYNIENINAHRADSDTLATAKL
                     FLKIKDEY"
     gene            733..1749
                     /locus_tag="PC471_003776"
     CDS             733..1749
                     /locus_tag="PC471_003776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HDF2790852.1"
                     /translation="MGYYNDNDNNKIRRKKISNSKDSNKKSKHSYTPANKKNKSSKTS
                     SSKRMSEKVDSQNLIKRDRDSFTRDNVRPLNTDYNQRERRRVEYKQREKKLKSKKRKK
                     QKRIRIVRALLSIMLVALIIFSVAGAIFTVSAIKGSPEVTKKLIRSKYISSEVVSIKQ
                     MPNDLKHAIVSIEDERFYKHKGVDVISLVRSVLNNVFTDTTQGASTIDMQVSKNLLTS
                     NDKTMKRKIRDMYNAKQMNKIMTKDEILEAYLNNMYLGKNAYGAAKGAEVYFGKKVSE
                     LNLAECAMLAGMTNNPARYIDHGEAKKRQVIVLYKMHQLGYITDKEYRIAKADDTPFK
                     SEID"
     gene            2103..2807
                     /locus_tag="PC471_003777"
     CDS             2103..2807
                     /locus_tag="PC471_003777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="B3/4 domain-containing protein"
                     /protein_id="HDF2790853.1"
                     /translation="MKKFVIEDAFWNLFPSAKIGVVICNDIDNSIKDEDYKKIILKGE
                     EEALKYLEDSEFSNNQVIKVWREAFKKFKTKKGARSSIEALLKRVHNGNNIGTINPLV
                     DIYNFISLKYALPCGGEDIDKFIGDIRLTRAIGNEEFIPLGTDENSFPYEGEIIYKDD
                     GGAICRCWNWREAVRTMLTEDTNNAFLCIELVDESRFKEFENALNELAKIVQEKLGGN
                     CKISILDIDNKEVSVD"
BASE COUNT         1269 a          283 c          529 g          926 t
ORIGIN      
        1 tgtaaaatct tcctatatca tataaaatta agtattgatt agtctattac ataaatatag
       61 aatataaata tatccattta ataataagga gaagagatat gaaagagtat gtagttgtgg
      121 atttagaaac tacaggatta gatccatata aaggttgtga aataatagaa ataggaataa
      181 cagaaataaa aaatgaacaa attgtaaaaa actattcaag gttgataaaa cctaagggga
      241 taatttcttc atttataaca gaattgacac atataagtaa tgaaatggta gaaaatgaag
      301 aaccactaga attagtttta cctagattta gaaaatacat aggtgataga actataattg
      361 cacacaatgc gaaatttgat ttaaaatttt taaattacta tcttagaatg ttagatttag
      421 aaccaataaa caattatata tgtacagtag agttattaaa aaaatgtaaa agttataaag
      481 gaaaaaacaa gaaacttgaa acagcttgtg cttattacaa catagagaat ataaatgctc
      541 atagagctga cagtgataca ttagcaactg caaaactatt tttgaaaata aaagatgaat
      601 attaattata aatatagaga aaattagtga aaatatcctt tataaaattg acccttaagc
      661 ttacaatttt gttctttgat ttattttgta gaacaatgta taatgtacct tatatgaaga
      721 agggagacta aaatgggtta ttacaatgat aatgataata ataaaataag acgtaaaaaa
      781 attagtaaca gcaaagatag taataaaaaa tctaaacatt cttatactcc ggcaaataaa
      841 aaaaataaaa gttctaagac aagttctagt aaaagaatga gtgaaaaagt agattcacag
      901 aatcttataa aaagagatag agatagtttt acaagagata atgtaagacc tttaaataca
      961 gattacaatc aaagagaaag aagaagagtc gagtataaac aaagagagaa aaaattaaaa
     1021 agtaaaaaaa gaaaaaaaca aaaaaggatt agaatagtta gagcattatt atctattatg
     1081 ttagttgcac taattatatt ttcagtggca ggtgcaatat ttacagtttc agctataaaa
     1141 gggtcaccag aagttactaa aaagttaatt aggtctaaat atataagtag tgaggttgta
     1201 agtataaaac aaatgccaaa tgatttaaaa catgcaatag tttccataga agatgagaga
     1261 ttttataagc ataaaggagt agatgttatt tctctagtta ggtcagtatt aaacaatgtg
     1321 tttacagata caacacaagg tgcaagtact atagatatgc aagtttcaaa aaacctgctt
     1381 actagtaatg ataaaactat gaagcggaaa ataagggata tgtataatgc taagcagatg
     1441 aataagatta tgactaagga tgaaattctt gaagcatatt taaataatat gtatctaggt
     1501 aaaaatgcat atggagctgc aaagggagct gaagtctact ttggtaaaaa agttagtgaa
     1561 ttaaatttag ctgagtgtgc tatgttagct ggtatgacaa ataatcctgc aaggtatatt
     1621 gaccatggag aggctaagaa gagacaagtg attgtactgt ataagatgca tcagcttggt
     1681 tatataactg ataaggagta tagaatagca aaagctgatg acactccatt taaatcagaa
     1741 attgattaaa attgtaagaa tatagtattt tcaatagttt aaacaaacaa aaatgtaata
     1801 aaaaaggtga tttgccaccc atttgccacc ataattataa tctaatggca aattgtttta
     1861 atgtacaaac tagataatag aatattgtct taaatgctat attatgctaa aatttaattg
     1921 tattttgtaa attaaaaaac ttataattta gttttctagg gttccgcatt ttataatggt
     1981 caggtccaag agaaaacgcg caattgttgt gtacacggaa ggataaaagc ctgggagata
     2041 tttaagaata tttctatagg cttttttatt tttacatatt ttaaatttga ggaggttttc
     2101 taatgaaaaa atttgttatt gaggatgcat tttggaatct atttcctagt gcaaaaattg
     2161 gagttgttat ttgtaatgat atagataact ctataaaaga tgaagactat aagaaaataa
     2221 ttttgaaggg agaagaagaa gcattaaaat acttggagga ctcagaattt agcaacaacc
     2281 aagtgattaa agtatggaga gaagcattta aaaaatttaa aacgaaaaaa ggagcaaggt
     2341 cttctattga agcattatta aaaagagttc ataatggaaa taatatagga actattaacc
     2401 ctttagtgga tatctacaat tttatctcat taaaatatgc attaccttgt ggaggggagg
     2461 atatagataa atttatagga gatattaggc taactagagc aattggtaat gaggagttta
     2521 tcccactagg gacagatgaa aattcatttc catatgaagg agaaataatt tataaagatg
     2581 atggtggagc aatttgtaga tgttggaatt ggcgtgaagc agtgagaaca atgcttactg
     2641 aagatactaa caatgccttt ttatgcattg aattagtaga tgaaagtaga tttaaagaat
     2701 ttgaaaatgc tttaaatgaa ttagctaaaa tagttcaaga aaaattgggt gggaattgta
     2761 aaatttcaat tttagatatt gataataaag aggtatcagt ggattaatat ggaggtgctg
     2821 ttgtaatgga ctattctagt tataaagata cttatattta gggtaagaag gtctatttta
     2881 gatataacga ttatcaaagg atataatata gaaaataaaa atgtagaata taaaaagcct
     2941 ttaaaataag aaaaaaatgt attttaaagg cttttattat actctttttt atttgcataa
     3001 ttaacaa
//
LOCUS       DAMJYR010000240         2987 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.245, whole genome shotgun sequence.
ACCESSION   DAMJYR010000240 DAMJYR010000000
VERSION     DAMJYR010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2987)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2987)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2987
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.245"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(41..988)
                     /locus_tag="PC471_003778"
     CDS             complement(41..988)
                     /locus_tag="PC471_003778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2790854.1"
                     /translation="MRITKTTFNDVFSNDANGSIIKVEKYVDKKCASVFEILTYTIIV
                     TNTSRYKTGNIFFKDYISKYIEFINNTVKVNGIIKRGLDPQQGFYIGRIDASCKKIIS
                     FKSVVLPNSAHRIIKNSANIYYYYKCNLDKFPTRISIESNRVYTNVNKIVFKQLNISN
                     TLKAPKNLRDILKVNVNSKIFDVKPMKNLINKGSNLKLCNLIVFGYIEVEIDYSCKNR
                     SNFKTVNKSNDIKKDKYYKNDIQNNNENINDCKKQISFGDNKVKKITKTFGFSCFICS
                     PVGIIYEDMKNINIKIEHTSINELNPEELFVNTSLLLYY"
     gene            complement(1016..1507)
                     /locus_tag="PC471_003779"
     CDS             complement(1016..1507)
                     /locus_tag="PC471_003779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2790855.1"
                     /translation="MYENNKVIYNLERCEKLEKTLYLSQFNTYKNFKLASKKTVINSL
                     ETYVYNMNINNISLVDTIECISLEDEQLTGKKLILVGDIDIDAILDYAGNKRNRNSSK
                     KNTFKLKIPFSTFIQMPRKIENKDKINLKYLIQDITSSILDDNLFISVTAIISYENSS
                     KIE"
     gene            complement(1950..2843)
                     /locus_tag="PC471_003780"
     CDS             complement(1950..2843)
                     /locus_tag="PC471_003780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HDF2790856.1"
                     /translation="MKNKKFIVIILIISILIGVLVKEYSSREIKKDDINVSKYIKYAD
                     LASKNNAQVNWKYVASIVAVLNKNNLKNVKDSQIQEVSDLFVKNFSKNNKINKLSDIL
                     DELEFSNRQKRLVDNYIDNLKDYGIKPERLKSDTKYMKFIDEIKTEAIQNYKDYKILP
                     SITIAQAIIESSWGKSTLAKQYNNLFGIKADAYWKGKSVTLETKEHLDTIIDDKFRIY
                     DDKNESIKDHAKFLATNKRYKNNGVFDAKTYIYQAKALEKAGYSTAKDENGNSIYAAR
                     LIELIQQYNLQLIDSEIQSEM"
BASE COUNT          996 a          419 c          266 g         1306 t
ORIGIN      
        1 aatgctattt taataagttt aagttttagc tttaaaatac ttaataatac aataaaagtg
       61 atgtatttac aaataattct tctggattta attcatttat tgatgtatgt tcaattttta
      121 tatttatatt tttcatatct tcataaataa ttccaactgg agaacaaatg aaacaagaaa
      181 atccaaaagt ttttgttatt ttttttacct tattatcacc aaaacttatt tgctttttac
      241 aatcgtttat attttcattg ttattttgta tatcattttt ataatactta tcttttttaa
      301 tgtcattaga cttatttact gttttaaaat tactcctatt tttacaacta tagtctattt
      361 caacttctat atacccaaaa actatcaagt tacataattt caaattacta cctttgttta
      421 taagattttt cataggttta acatcaaata ttttactatt tacatttact tttaatatat
      481 ctcttagatt tttaggagct tttaaagtat tacttatatt caattgtttg aatacaattt
      541 tatttacatt agtataaact ctattacttt ctattgatat tcttgttggg aatttatcta
      601 gattacactt ataataatag tatatattag cactattctt tataatccta tgtgcagaat
      661 ttggtaaaac tacactctta aaagatatta tttttttaca acttgcatct attctcccta
      721 tgtaaaaacc ttgctgtggg tctaatccac gttttataat gccattaact tttaccgtat
      781 tatttataaa ctctatatat ttagaaatat aatctttgaa aaatatattt ccagttttgt
      841 acctacttgt atttgtaaca ataatagtgt aggtcaaaat ctcaaataca gatgcacatt
      901 ttttatcaac atatttttct acttttataa tacttccatt tgcatcattt gaaaatacat
      961 cattaaaagt agttttcgtt attctcattg catccacctt tctataattt aaagattact
     1021 ctatcttgga actattttca taacttataa ttgcagtaac actaataaat aaattatcat
     1081 ctaaaataga tgaagtaata tcttgtataa gatactttaa attaatttta tctttatttt
     1141 ctatctttct aggcatttgt ataaatgtac taaagggtat ttttaattta aatgtatttt
     1201 ttttactaga atttctattt cttttattgc ctgcataatc taatattgca tcaatatcta
     1261 tatcaccaac taaaatcaac ttctttcctg taagttgttc atcttctaat gatatacatt
     1321 ctatagtatc aacaagactt atattgttaa tattcatatt gtaaacatat gtctctaatg
     1381 aatttattac agttttttta gaagctaatt taaaattttt atatgtatta aattgagata
     1441 aataaagagt cttttctagt ttttcacatc tttcaaggtt gtatattacc ttattatttt
     1501 catacataat cgcatcaatc tccctgtatc tatatattaa tactattcat aaaaattatt
     1561 tcaaattacc attttttatt attcactatc tagttgttat ttacttttaa gaaagaatcc
     1621 ttttttactt cattgatttc aatttacaag taaaagtctt tctgaaaaaa aaatatatat
     1681 atctctattc acttttttat aagtaattat aatttttata gactaacggt cagccttaag
     1741 ggaaaaggtg ttctttaagt gcagaattta gataaagtaa ataatccttt tataaataat
     1801 tccaagtttt aacttaaata atttgattta cagtataaag taaattttga aagataatga
     1861 tatcgattta ataattacaa gtaaaagcat gtcaaaataa ggctatgatt ttaaaaatca
     1921 tagccttatc aataaaaatt taatcaaagt tacatttcac tttgaatttc actgtctatt
     1981 aattgaagat tatactgttg aattaattct attagacgag ctgcatatat actattgcca
     2041 ttttcatcct tcgcagtact ataacctgcc ttttctaatg ctttagcttg atatatatac
     2101 gtttttgcat caaatacacc attgttttta taccttttat ttgtggctaa aaattttgca
     2161 tggtctttta tagattcatt tttatcatca tatattctaa atttatcatc tataattgta
     2221 tctaaatgtt ctttcgtttc taaagtcact gacttgcctt tccaataagc gtcagccttt
     2281 ataccaaata gattgttata ttgttttgcc aaagttgact tgccccagct agattctatt
     2341 atagcttgtg caatagttat agatggaagt attttataat ccttataatt ttgtattgct
     2401 tctgttttaa tttcatcaat aaacttcata tattttgtat cagacttcaa tctttctggt
     2461 tttataccat aatccttaag attgtctata taattatcaa ccaatctttt ttgtctatta
     2521 ctaaattcta actcatccaa aatatcactt aacttattta ttttattatt tttagaaaaa
     2581 ttcttaacaa ataaatctga tacttcttgt atctgtgaat ccttaacgtt ttttaagtta
     2641 tttttattta acacagcaac aatggaagct acatacttcc agttaacttg tgcattattt
     2701 ttacttgcta aatcagcata ttttatatac ttactgacat ttatatcatc cttttttatc
     2761 tctctagatg aatattcctt cactaataca cctattaaaa ttgatattat cagtattata
     2821 acaatgaatt ttttattctt cataaatatt gtcctccctt atactatctg ctgaatataa
     2881 tataccttac ttttgtgaca tattctttac gaaattttga aaattttatg aagatttatg
     2941 aaatatattt aattaaaagc actactattg ttataatata tagtttt
//
LOCUS       DAMJYR010000241         2979 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.246, whole genome shotgun sequence.
ACCESSION   DAMJYR010000241 DAMJYR010000000
VERSION     DAMJYR010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2979)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2979)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2979
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.246"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            111..479
                     /locus_tag="PC471_003781"
     CDS             111..479
                     /locus_tag="PC471_003781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HDF2790857.1"
                     /translation="MENLVKIFKALSDETRLNILILVSKRNICQKGISKYLGISDSAV
                     SQHIKILKDVGIITGYKEGYYVLYHINKESFNICVDFINSMLSNSSESFIDVFDVNTI
                     HLGCSKECKSIKKCCKRREK"
     gene            482..853
                     /locus_tag="PC471_003782"
     CDS             482..853
                     /locus_tag="PC471_003782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HDF2790858.1"
                     /translation="MKVCIPVEENKGLDSKPYGHFGSAPIFVVCDLESGEVKSLDNGD
                     LDHEHGKCQPLKALSGTAVDAVVVGGIGQGAIIKLNGMGIKVYRAEGDTISANLDLLK
                     NGKLVEFPSDHTCSHDGCGHH"
     gene            complement(998..1453)
                     /locus_tag="PC471_003783"
     CDS             complement(998..1453)
                     /locus_tag="PC471_003783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2790859.1"
                     /translation="MNINTVQGDSIEVLLRQLGATRISKVSSTLYFIKFDLGDGWEIS
                     YTYNINAKDQYFLQRIEPYPIGRGLFNDEYEIVSFISKDLKKFLNAKNSSNFKTFVEV
                     TRKVNSIIDNVEELFLNYNVDGDDLKTLNKELNDILNDIDYIEKHTKKI"
     gene            complement(1492..2757)
                     /locus_tag="PC471_003784"
     CDS             complement(1492..2757)
                     /locus_tag="PC471_003784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsB/NhaD family transporter"
                     /protein_id="HDF2790860.1"
                     /translation="MEFQQIIAICIFLIVMAAIITEKVNRSVAAVGGALLMIIFNILT
                     LDEGLSHIDFNTIGVLVGMMLFVAVVKNSGLFEYIAIWTAKKAKGDPWKIMICFAIIT
                     AILSAVLDNVTTVLLIGPMTIVITQILGLNPVPFLITQILASNIGGTATLIGDPPNIM
                     IGSAANLSFMDFVINLGPAVIVILAITIICFRFIYGKELVVNERAKNAILKLDEKKSV
                     KDKPLLIKSLILIAFILFGFMFHSTIHIDSSVVALTGASIMLLIGKQDVDEIMAGIEW
                     STILFFMGLFVVVGGLVEVGIINKLAQALIGLTEGHLVFTMLLILWLSAIVSSFLDNI
                     PFVATLIPLILTMQAEGIDVMPLWWATSLGACLGGNGTLIGASANVVLAGIGNKHGHP
                     ISFKEYFKIGFPLMIISIIISTVYLIIKF"
BASE COUNT         1109 a          413 c          408 g         1049 t
ORIGIN      
        1 aaattggaat tatttataat atactccaaa atttaaaaat aatggttgaa aaatctaatt
       61 aagagtacta taatttaatt aagtaattac ttaatcaaaa tgaggtactt atggaaaact
      121 tggttaaaat atttaaagca ttgagtgatg aaactagact taacatactt atattagtat
      181 ccaagagaaa tatatgtcaa aaggggattt ctaaatatct agggatttca gattctgctg
      241 tttcacaaca tataaaaata ttaaaagatg ttgggataat aacaggatat aaagaaggat
      301 actatgtttt atatcatatt aataaagaat catttaatat atgtgtagat tttataaatt
      361 ctatgttaag taattctagt gaaagcttta tagatgtatt tgatgtaaat acaatacatc
      421 taggatgcag taaagaatgt aaatctataa aaaaatgttg taagagaagg gagaaataat
      481 aatgaaagta tgtataccag tagaggagaa taaaggattg gatagtaaac catatggaca
      541 ttttggttct gctccaattt ttgttgtgtg tgatttagaa agtggagaag ttaaatcttt
      601 agataatgga gatttagatc atgaacatgg aaaatgtcaa ccattaaaag cattgtcagg
      661 gacagctgta gatgcagtag ttgttggagg aataggacaa ggagctataa ttaaattaaa
      721 tggaatggga ataaaagttt acagagcaga aggtgatact ataagtgcta atttagactt
      781 acttaaaaat ggaaaattag tagaatttcc atcagaccat acttgctcac atgacggatg
      841 tggacatcat taaaaaaaaa cagctgatga aatatatact tcatcagcta ttttttcttt
      901 taataaatac gattttattc ttttaaaacc aaaatttaga tttttatgtt ttttgtagta
      961 tccttaataa tatttcaagt ttacaagttt actactttta gatttttttt gtatgttttt
     1021 ctatatagtc aatatcattt aatatgtcat tgagttcttt attaagtgtt tttaaatcat
     1081 caccatctac attgtagttt aaaaatagtt cctctacatt atcaatgata gaattgactt
     1141 ttctagtaac ttcaacaaat gtcttaaagt tactactatt tttagcattt agaaattttt
     1201 ttaaatcctt tgatataaat gatactattt catattcatc attaaataga cctctaccaa
     1261 taggatatgg ttcaattctc tgtaaaaaat attggtcttt agcattaata ttataagtat
     1321 aagatatttc ccaaccgtct cctaaatcaa acttgataaa atataatgta ctacttactt
     1381 ttgatattct tgtggcacca agctgtctta aaagaacttc aatcgaatca ccttgaacag
     1441 tattaatatt catatataca catccccttt agtattatta tactatattt tttaaaattt
     1501 tataattaaa taaacagtag atataataat agatataatc ataagtggaa aacctatttt
     1561 gaaatactcc ttaaaagaga taggatgacc gtgtttatta cctatacctg ctagtacaac
     1621 attagcagat gctcctatta gtgttccatt tcctccaaga caagctccaa gagatgttgc
     1681 ccaccatagt ggcattacat caataccttc agcttgcata gtcaaaataa gaggaattag
     1741 tgttgcaaca aatggaatat tatctaagaa agaagagact atagctgaca accataatat
     1801 caacaacatt gtaaatacaa gatgtccttc tgtaagtcca atcaatgctt gagccaattt
     1861 gtttattatc ccaacttcta ctaatccacc aactactaca aataatccca taaagaataa
     1921 aattgtagac cattcaatac ctgccataat ttcatctaca tcttgttttc ctattaaaag
     1981 cattatagat gctcctgtca gtgctacaac agaagaatct atgtggattg tactgtgaaa
     2041 cataaaacca aaaagtatga atgctattaa tataaggctt tttataagta gaggtttatc
     2101 tttaacagat tttttttcat ctagttttaa tattgcattt ttagctcttt cattcacaac
     2161 aagttctttt ccataaataa atctaaagca tattatagtt atagcaagta ttactataac
     2221 agcaggacct aaatttatta caaaatccat aaaactaaga ttggcagcac taccaatcat
     2281 aatatttgga gggtctccta taagagtagc tgttcctcct atatttgaag ctaaaatctg
     2341 tgttattaaa aatggaactg gattaagtcc taatatttgt gtaataacta tagtcattgg
     2401 tccaataagt aagacggttg taacattatc taatacagct gataaaatag ctgttataat
     2461 agcaaaacaa atcattatct tccatgggtc acctttggct ttctttgcag tccagatagc
     2521 tatatattca aataaccccg agtttttgac aacagctaca aatagcatca taccaactaa
     2581 tacaccaata gtattgaaat ctatatggct taatccttca tcaagtgtaa gtatattgaa
     2641 tattatcatt aatagtgcac caccaacagc tgctacggat ctgtttacct tttctgtaat
     2701 aatagctgcc ataactatta aaaatataca tatagctatt atttgttgaa attccataat
     2761 ataagtacct ccatttgtat tattttgtgc ctaatgctag tactatatat agtattactt
     2821 ttttattgtt ttttcaatgg atgtatgaaa gtatattatt taacaaagtt tttggaaaat
     2881 tgacagaatt taaagaaaat ttttgttatt taaataaatt ttaaaattat tccattgata
     2941 ataatattaa acgacaaacg aaaaaatata ccctttaaa
//
LOCUS       DAMJYR010000242         2920 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.247, whole genome shotgun sequence.
ACCESSION   DAMJYR010000242 DAMJYR010000000
VERSION     DAMJYR010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2920)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2920)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2920
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.247"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            111..1220
                     /locus_tag="PC471_003785"
     CDS             111..1220
                     /locus_tag="PC471_003785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HDF2790861.1"
                     /translation="MDFKSLGISENTINILKKSGITTPTPIQKESIKLIKEGKDVIAE
                     AQTGTGKTLAFLLPIFENISLDINDIQVLILSPTRELAIQITEEAMKLKESKDVSILA
                     AYGGKDIGSQIKKLKGNIHMIIATPGRLLDHLNRKTIDLSKLKTFVLDEADQMLLMGF
                     KNEVEAILKETSNKKQTLCFSATINSQVKKLAYRYTKNPVVVSIQKEEITLNNIKQEV
                     VETTDRKKLDALCKVLDEDNPFMAIIFCRTKRRVDNLEEALAIRGYNCQKLHSDIAQS
                     KRERIMKSFRNLDIQYLIATDVASRGLDISGVSHIYNYDLPETPEDYIHRIGRTGRAG
                     EEGYTCAFIDPKNERMLSEIETAIESKISRRIIEL"
     gene            complement(1597..2781)
                     /locus_tag="PC471_003786"
     CDS             complement(1597..2781)
                     /locus_tag="PC471_003786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2790862.1"
                     /translation="MQKSVKKAKVTGSMLAIFGVASVLFSSHAGGGFATGNQETQYYV
                     QYGWTAPLMAILAMIILTATMREVIIMYNNNNCRNYKDLFCELWRPYPKLEIIWEIYY
                     YLMVLIAVSAVIAGAAAVFQSIGVNYFVAVFIIGVVLLVFTIFGAMLVSKAATAMTIA
                     ILVCTLTIFIVGIKAKVPEITEILSNRTSFTPGYIKPILNTFIYAGFQSVVIPTLAGC
                     SRPLLKNSKEATKAMIISFVMNAIALGLAVTMLMGWYREIIAAGQTTLPTLYVAGQSG
                     NHTIYIIYNVALFLCLMSTGVTTIFGLVNRFEDHKALSFLSSRMKRRVFTACAIMVVS
                     MLISLTGLSNIVKYGYGYCGYLGLFTIVIPFLTLGHYKNKKFAKENPEAKWPAELNEN
                     VN"
BASE COUNT         1120 a          442 c          403 g          955 t
ORIGIN      
        1 tttttaagct atataaataa taattaaaaa atttactaaa aatacttaca aatatgatat
       61 tattaattgg ttaataaaaa agaaaatttt tcatgaaagg taagataaca atggatttca
      121 aaagcttagg gattagtgaa aatacaataa atattctaaa aaaatcagga ataacaacac
      181 ctacacctat acaaaaagaa agtattaaac taataaaaga aggaaaagat gtaatagcag
      241 aagctcaaac tggaacagga aaaacactag cttttttact tccgatattt gaaaatatat
      301 cattagatat aaatgatata caagtactta tactttctcc tacaagagaa ctagctattc
      361 aaataacaga agaagctatg aaacttaaag aatctaaaga tgtaagtatt cttgcagcat
      421 atggaggaaa agatattggt tctcaaataa agaaactaaa aggaaatatt catatgatta
      481 tagctacacc aggtaggctt ttagaccatc taaatagaaa aacaatagac cttagtaagt
      541 taaagacttt tgttttagat gaagcagacc aaatgttgct tatgggattt aaaaatgaag
      601 tggaagctat tttaaaagag acttcaaaca aaaagcaaac actatgcttt tctgcaacta
      661 taaattctca agtaaaaaaa ttagcttata gatacacaaa aaatcctgtt gtagtttcta
      721 ttcaaaaaga agaaatcact cttaataata taaaacaaga agtagtagaa actacagata
      781 gaaaaaaatt agatgcctta tgtaaggtac tagatgaaga taatcctttt atggcaataa
      841 tattttgtag aactaagaga agggttgaca atcttgaaga agctcttgct ataagaggat
      901 ataattgtca aaaacttcat agtgatattg ctcaatcaaa acgagaaaga ataatgaaat
      961 catttagaaa tttagatata caatacttaa tagctacaga tgtagcttct cgtggtcttg
     1021 atatatctgg agtaagtcat atatataatt acgatttacc agaaacacca gaagactaca
     1081 tccaccgtat tggtagaact ggtagagctg gtgaagaagg ctatacatgt gcttttattg
     1141 accctaaaaa tgaacgaatg ttaagtgaaa tagagacggc tatagaatct aaaattagta
     1201 gaagaattat agaattataa tttatagatt gcttaaaata ccaatgccac acaaacactg
     1261 taatatatag aaagctacag aaaacatgta tcaatctata ctttgctgtt atactatttt
     1321 atattaaaga aaaaagaggc tatccaaagt aggattagtc tcttttattg tcaaaataat
     1381 gaaaatatat ttgacttcat ataaaaaaga gtgccttata aactaaaagt ccattttgag
     1441 ataccctctt tttatctttt attcttagtt ttatattgct tgtttatagt tgtttaatat
     1501 tagattttgc aaataatctt aataaacatg cagatatatt atctatagta ttcttattta
     1561 tagtattctc tttagcaata ttttttgcta ctacatctaa tttacatttt catttaattc
     1621 cgctggccat ttagcttctg gattttcctt tgcaaacttc ttattcttat aatgtcctag
     1681 tgtaagaaat ggaattacta tagtgaatag accaagataa ccacaatatc catatccata
     1741 tttaacaata ttacttaatc ctgtaagtga tataagcatt gacactacca ttattgcaca
     1801 agctgtaaat acccttcttt tcattcttga agaaaggaaa gaaagtgctt tatggtcttc
     1861 aaatctattt acaagaccaa atatagtagt tactcctgta gacattaaac aaaggaataa
     1921 tgcaacatta taaattatgt atatagtatg atttccagat tgacctgcaa cataaagagt
     1981 agggagtgtt gtttgcccag cagcaataat ttctctatac catcccatta gcatagttac
     2041 agcaagtcct aatgcaattg cattcataac aaatgaaatt atcattgcct tagtagcttc
     2101 ttttgagttc ttaagaagtg gtcttgaaca tcctgcaagt gtaggtataa caaccgattg
     2161 aaaacctgca taaataaaag tattaagtat tggcttgata tagccaggtg tgaaactcgt
     2221 tctatttgat aatatttcag ttatttctgg tactttagct tttataccaa caataaaaat
     2281 agtaagagta catactagaa tagctattgt cattgctgta gctgctttag atacaagcat
     2341 agcaccaaat atagtaaaga caagaagtac aactccaata ataaatacag ctacaaaata
     2401 atttactcct atactttgaa atactgctgc tgcacctgct ataacagcac ttacagctat
     2461 taaaaccatt agatagtaat atatctccca tattatttca agttttggat atggtctcca
     2521 aagctcacaa aataaatcct tataattcct acaattgtta ttattataca ttatgataac
     2581 ttctctcatg gtagcagtaa gtataatcat tgctaaaatt gccataagtg gtgctgtcca
     2641 tccatactgc acatagtact gagtttcttg attccctgta gcaaatcctc ctcctgcatg
     2701 tgaactaaaa agaacgcttg caacaccaaa aatagctaac atagagcccg ttacttttgc
     2761 tttctttaca cttttttgca tgaaatcatc ctccctaaaa tgatattgaa agtttttatt
     2821 ttaacacttt attgataaaa caataacatt tttgctcatt aaattcaact atatttttcc
     2881 aatgacttta cattttcaac ttatttccac ctttatatta
//
LOCUS       DAMJYR010000243         2895 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.248, whole genome shotgun sequence.
ACCESSION   DAMJYR010000243 DAMJYR010000000
VERSION     DAMJYR010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2895)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2895)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2895
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.248"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(<1..213)
                     /locus_tag="PC471_003787"
     CDS             complement(<1..213)
                     /locus_tag="PC471_003787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HDF2790863.1"
                     /translation="MKNFILIYFIVINSIAFFSMYIDKKRAIRNEWRIKEATLMSIAV
                     IGGSIGSIIGMYSFRHKTKHIKFTFGI"
     gene            440..1141
                     /locus_tag="PC471_003788"
     CDS             440..1141
                     /locus_tag="PC471_003788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2790864.1"
                     /translation="MKFIVEKEVFDKLENVCFGVVVAKGIDNTKEIERISNLLDISID
                     RVEDYFKDKKVKESEEIIPYREAFRSLGMNPNKFMSSIEAMTTRVAKNKKLPHINPIV
                     DLGNSISLKYLLPMGAHDMDFRNDDVYVRFSKKGDKFVPFGETDVELMEEGELIYSVG
                     DMVKTRRWIWRQGEEGKITNSSKNIFFPIDGFTDANLDKVMSAREELAKLLKEIFNCE
                     IKVGFVDKDNPEMEI"
     gene            1223..1831
                     /locus_tag="PC471_003789"
     CDS             1223..1831
                     /locus_tag="PC471_003789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HDF2790865.1"
                     /translation="MKKTIIKNVNPMIENKFIGLFEIEYKNKLDEDKVWMVASRKSSE
                     QLKNIYLKNEEDSVDAVVIVGLHKSSKKLILIRQFRVPINGYIYELPAGLVDEGESID
                     ISVERELREETGLTLLEIKKNKSIDKVYLSPGMSDESVAFVYCICDGDITDEFLEPDE
                     EIEALLISQEDAKEILQSNHKIDTKAFLILQMFVSLGAKLFE"
     gene            2155..2619
                     /locus_tag="PC471_003790"
     CDS             2155..2619
                     /locus_tag="PC471_003790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4624 family lipoprotein"
                     /protein_id="HDF2790866.1"
                     /translation="MKKLVIILISIISMVSLTACKKSNESKYNNLNNKESGQTTIEMD
                     LDKNYDTSDPFVNARLFCVSNDIDVLDTEISFKMDGDSGIVEIKDNKTYETLWSNTWH
                     GSVDNDTFTISLSNIQKEKEYAICFTGIKINHAVVKVTFESNLVKEKEKPSK"
BASE COUNT         1198 a          248 c          487 g          962 t
ORIGIN      
        1 tataccaaat gtaaatttaa tgtgtttagt tttatgtcta aaagaataca ttccaattat
       61 agaacctata ctacctccaa ttactgctat agacataaga gtagcttctt ttattctcca
      121 ttcgtttcta attgctcttt tcttatctat gtacattgaa aagaaagcaa tagaattaat
      181 cactataaaa tatattagaa taaaattttt cacagtcttg cctccataaa aatatattat
      241 acattataaa acaagatatt tgttttggaa agttaaattt gtaattagta catataaaat
      301 atttgataag aacaaatata atattaattg gaaaatttaa aaaaattaaa caattagcaa
      361 atagggggta tatttataag acacaaagtg ataaaatcta tttagaaaat aatgtaaaat
      421 aacaggagga taaagcaaaa tgaaatttat agtagaaaaa gaagtttttg ataaattaga
      481 aaatgtatgc tttggtgtag tagttgcaaa aggaatagat aatactaaag aaatagaaag
      541 aataagcaac cttttagata taagtataga tagagtagaa gattacttca aagataaaaa
      601 agtaaaggaa tcagaagaaa ttataccata tagagaggca tttagaagtc taggcatgaa
      661 cccaaataaa tttatgagtt cgatagaagc aatgacaaca agggttgcta agaataagaa
      721 acttcctcat ataaatccta ttgtagattt aggaaactct atttctttaa aatatttact
      781 tccaatgggt gctcatgata tggattttag aaatgatgat gtatatgtta gattttctaa
      841 aaaaggggat aaatttgtac catttggaga aactgatgtt gaattaatgg aagaaggaga
      901 attaatttat tcagttggag acatggtaaa aactagaaga tggatatgga gacaaggaga
      961 ggaaggaaaa attactaata gctcaaaaaa tatatttttt cctatagatg gttttactga
     1021 tgctaattta gataaggtaa tgagtgcaag agaagaatta gcaaaattgt taaaggaaat
     1081 atttaattgt gaaattaaag taggttttgt agataaagat aatcctgaaa tggaaatata
     1141 ataagttgtt gaatacttgt taagatatta tatttactta atgatgttaa aaaggtatta
     1201 aaaaagaaag gtcgtagtaa atttgaaaaa aactataata aagaatgtaa atccaatgat
     1261 tgaaaataag tttataggac tttttgaaat agagtataaa aacaaattag atgaagataa
     1321 ggtttggatg gtagcatcaa gaaaaagtag tgagcaatta aaaaatattt atttgaagaa
     1381 tgaagaagat agtgtagatg cagttgtaat agttgggctt cataaaagta gtaaaaaatt
     1441 aattttaata agacaattta gagtacctat taatggatat atatacgagt taccagcagg
     1501 tcttgttgat gaaggagagt ctatagatat ttccgttgaa agagaactta gagaagaaac
     1561 aggacttact cttttagaaa ttaaaaaaaa taaaagtatt gataaagttt atttatctcc
     1621 tggaatgagt gatgaatctg ttgcctttgt ttattgtata tgtgatggtg atataacaga
     1681 tgaattttta gaaccagatg aagagattga agcgctttta atttcgcaag aagatgcaaa
     1741 agagatttta caaagtaatc ataagataga cacaaaagca tttttgattt tacaaatgtt
     1801 tgtgagttta ggagctaaat tatttgaata gatatataga aaagaggtta tatagaagag
     1861 taaaataata aagtttattt tacttgtata aattataatc tcttttttta tacaataaag
     1921 tataattttg tgtattttta aataagtgac ttgactctta aattttcata atatgttata
     1981 aaccaattta agatactaca ataattaaaa ttccaaaatt atttaacata tttgcaatta
     2041 aaatgagaaa aggtaaaggt tgaatgattg aaaaatttga tgtaaaatag tagcttagtt
     2101 taatattata gtttgtaggg ttaaaaatta gaaattgtaa gggagataaa ttctatgaaa
     2161 aaattagtta ttattttaat tagtatcatt agtatggtta gcttgacagc ttgtaaaaaa
     2221 agcaatgaaa gtaaatataa taatttaaac aacaaagaat ccggacaaac aaccattgaa
     2281 atggacttag ataaaaatta tgatacttca gacccatttg ttaatgcacg tttgttttgt
     2341 gtgtctaatg atatagatgt tttagacaca gagatttctt ttaaaatgga tggagatagt
     2401 ggtattgtgg aaataaagga caataaaaca tatgaaactt tatggagtaa tacatggcat
     2461 ggaagcgttg ataatgatac attcacaatt tctctttcta atatacaaaa agagaaagag
     2521 tatgctatat gctttactgg aattaaaata aatcatgcag ttgtaaaggt tacttttgaa
     2581 agcaatttag ttaaggagaa agaaaaaccc tcaaaataaa ttccagattg tctataactt
     2641 gatattcagt acataaaagc agttgatatt tatgattgac tgctttttct tatcaaaaag
     2701 gagagtttgt gcatttttac ttgaaatagt ttatgcacat caaaagttgg tgagcatgat
     2761 attggaaatg gtaagcatga aatcttgata agacaaggaa ataaatttaa gttttcaatt
     2821 aagtatattt ccataattta gttaacaata agctaattat tatttatatt ttgatatata
     2881 aataataatt gtagc
//
LOCUS       DAMJYR010000244         2817 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.249, whole genome shotgun sequence.
ACCESSION   DAMJYR010000244 DAMJYR010000000
VERSION     DAMJYR010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2817)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2817)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2817
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.249"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(71..511)
                     /locus_tag="PC471_003791"
     CDS             complement(71..511)
                     /locus_tag="PC471_003791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3788 domain-containing protein"
                     /protein_id="HDF2790867.1"
                     /translation="MYERMLDKSNKPCLSQIIEYIGKDGYARLCKLETYLKTQYNLSR
                     ELRFPFGNNYGWGYKYSHKSFHLFYAFFEKGAFTVLIQIGDKQVPAVEKTLVALTPKA
                     KELWVTRYPCGNNGGWIKYRILEDADLLDIIQYIRAKKSPPKQN"
     gene            866..2179
                     /locus_tag="PC471_003792"
     CDS             866..2179
                     /locus_tag="PC471_003792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
                     /protein_id="HDF2790868.1"
                     /translation="MDKRYLESDVFEAFQERLSYLFNEFDYIYVSFSGGKDSGLLLNL
                     VLDYKRKNNISSKIGVFHQDFEAQYSLTTEYVEKVFEDNIEDIEPYWVCLPMATRTAV
                     GNYEMYWYPWDDEKEELWVREMPVKEYIINQKNNPFTYYKYKMRQESLAKQFGRWIRD
                     IHEGAKVVCLLGTRAAESLQRYCSIVNKQHGYKGKCWITKEFKNVWTASPIYDWEIED
                     IWLAHYKFDYDYNKIYDLFYKAGVAPRKMRVASPFNDSAKESLNLYRILDPDVWVKLV
                     ARVQGVNFTSIYGKTKAMGYRSVKLPYGHTWKSFTYFLLDTLPENIRNSYIKKFSTSI
                     EFWQNTGGGLSEIVIRELLRKGYKIELNGVSNHTKDKKSRVIFLDDIPDDTDDIKFTK
                     DIPSWKRMCYCILKNDHMCRFMGFGPTKEQRIRTEYIKKKYEKIH"
     gene            2197..2733
                     /locus_tag="PC471_003793"
     CDS             2197..2733
                     /locus_tag="PC471_003793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB-like nuclease domain-containing protein"
                     /protein_id="HDF2790869.1"
                     /translation="MEYKSPVYGIIAVPIEKIESNDYNPNAVAPPEMQLLYKSIKEDG
                     YTMPIVCYYNEEKDKYIIVDGFHRYRVMLENEDIYDRENGLLPVSVIDKSLDERMAST
                     IRHNRARGSHNVDLMSKIITELCEIGKSDEWISKHLGMEVDEILRLKQITGLAYLFKD
                     KEFGNAWIPFGSDIEEEN"
BASE COUNT         1082 a          299 c          496 g          940 t
ORIGIN      
        1 tttatattat tttttaatat ggaaagaatg gaatttatat acactatatt taaatcacaa
       61 aaaataaaag ttaattttgt ttaggtggag atttttttgc tcttatatat tgaataatat
      121 caagtaagtc tgcatcttct aaaatacgat acttaatcca gcctccatta ttgccacaag
      181 gataacgagt gacccagagt tctttagctt ttggtgtcaa agctaccaac gttttttcta
      241 ctgctggaac ttgcttgtca cctatctgaa tcagaacagt aaatgcacct ttttcaaaaa
      301 aagcataaaa taaatgaaaa gatttatggc tatatttgta accccaacca taattgtttc
      361 caaaaggaaa tcgaagttct cttgataaat tatattgagt tttcaagtaa gtttccaact
      421 tacacaatcg tgcatatcca tctttaccta tatattctat tatttgggac aaacagggtt
      481 tattgctttt atcaagcata cgttcataca ttagaattta cctccatttt tacttttaat
      541 tatagcaaag taattatgac aacagtatgt caagttttta tgcattattc aagtttttat
      601 gtattattat actaatacgt agaaatataa agtaattagt tttagctaat agaaaaatat
      661 gatatttata tatattggaa aaaaatgtaa aataatgata aaatatagtt tataatacaa
      721 aagtataatt acattataat tatatgtagt tagttggatt tgtgtattta aataatgtat
      781 aatttatgct gattactgct aaaaaagcat aataatattg cattgtaagg ttttataaac
      841 ttctaattga aaggggataa ttcaaatgga taagagatat ttagaatccg atgtatttga
      901 agcttttcaa gaaagacttt cttatctttt taatgaattt gattatatct atgtatcttt
      961 tagcggagga aaagatagtg gtttactgct aaatttagtt ttggattata agagaaagaa
     1021 taatataagt tccaaaatag gagtatttca tcaagatttt gaggctcaat atagtttgac
     1081 tactgaatat gttgaaaaag tatttgaaga taatattgaa gatattgaac catattgggt
     1141 gtgtttacca atggctacaa gaactgctgt aggaaattat gaaatgtatt ggtatccatg
     1201 ggatgatgag aaagaagagt tatgggtaag ggaaatgcca gtcaaagaat acataataaa
     1261 tcaaaaaaat aatcctttta cttattataa atataaaatg agacaagaaa gtttggcaaa
     1321 acaatttggt agatggatta gagatataca tgaaggtgca aaagttgttt gcttattagg
     1381 aacacgagca gcagaatctc tacaacgtta ttgttctatt gtgaataaac agcatggata
     1441 taaaggaaag tgctggataa ctaaggaatt taaaaatgtc tggacggcat ctcctatata
     1501 tgattgggaa atagaagata tatggttagc acactataaa tttgactatg actataataa
     1561 aatttatgac ttattctata aagctggagt agctccaaga aaaatgagag tggcatcacc
     1621 ttttaatgat tcagcaaaag agagtttaaa tttatacaga atattggacc cagatgtatg
     1681 ggtgaaattg gttgctagag tacaaggagt taattttact tctatttatg gcaaaacaaa
     1741 agctatggga tatcgtagtg ttaaattgcc atatggacac acatggaaaa gtttcacata
     1801 ttttttgctt gataccttac ctgaaaatat aaggaatagc tatattaaaa aattttcaac
     1861 atctattgag ttttggcaga atacaggtgg tggtttgtca gaaatagtta tacgtgaatt
     1921 gttgagaaaa ggttataaaa tagaattaaa tggagtttca aatcatacaa aagataaaaa
     1981 gagtagagtc atatttttgg atgatatacc tgatgatacg gatgatatta aatttaccaa
     2041 agatatacca agttggaaaa gaatgtgtta ttgtatttta aaaaatgacc atatgtgtag
     2101 atttatggga tttggtccta caaaagaaca gagaattaga acggaatata ttaagaagaa
     2161 atatgaaaaa attcattaga taggagttaa gatgctatgg aatataaaag tcctgtatat
     2221 ggaatcatcg cagtgccaat cgaaaagata gaatctaacg actataatcc aaatgcagta
     2281 gccccaccag aaatgcagtt gttgtataaa agtataaaag aagatgggta tacaatgcca
     2341 attgtctgtt attataatga ggaaaaggat aagtatatta ttgtagatgg ttttcataga
     2401 tatagagtaa tgcttgaaaa tgaggatata tatgatagag aaaatggact tcttcctgtt
     2461 tcagtaattg ataaatctct tgatgaaaga atggcaagta ctattcgaca caatcgtgca
     2521 agaggttctc ataatgtgga tttaatgagt aaaattataa ctgaactttg tgaaattgga
     2581 aaatctgatg aatggatatc aaagcacctg ggtatggaag ttgatgaaat tcttagattg
     2641 aagcaaataa ctggattagc ttatttattt aaagataaag agtttggtaa tgcatggatt
     2701 ccctttggct ctgatataga agaagaaaat taaatagatt tattaaaaga aaaagattta
     2761 ttaaaaaaga aaatttaaaa tatatattaa aaaataaaga gttttaatat tatatgt
//
LOCUS       DAMJYR010000245         2815 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.250, whole genome shotgun sequence.
ACCESSION   DAMJYR010000245 DAMJYR010000000
VERSION     DAMJYR010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2815)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2815)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2815
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.250"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            23..799
                     /locus_tag="PC471_003794"
     CDS             23..799
                     /locus_tag="PC471_003794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HDF2790870.1"
                     /translation="MEYIIFDLEFNQGFDKKLNKTVSNEKCPFEIIQIGAIKLDSKFN
                     IIDTFNSYVKPTIYKDIHPFISRMTNIKSSDFNDSPTFPEVYNNFIKFISSQDPILCV
                     WGAGDLKELYRNINYHKLPSNSLPKSYINIQQHASKYFNNPAGKSIGLQNAISILELD
                     EKMSYHNALNDAYYTAKVFIKIYNPSIVPDIYLYTSIKPKTIRYSNKKRVDYDKLFDE
                     FRKILNRELTKDEKKIINLAYNMWKTNQFTLENVKQRKNK"
     gene            complement(891..1643)
                     /gene="xth"
                     /locus_tag="PC471_003795"
     CDS             complement(891..1643)
                     /gene="xth"
                     /locus_tag="PC471_003795"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HDF2790871.1"
                     /translation="MKFISWNVNGIRACVGKGFLDFFKEVDADIFCLQETKLQEGQIE
                     LDLPGYFQYWNYAERKGYSGTAIFTKKEPLKVMYGINIEEHDKEGRVITLEFEDFYFV
                     TVYTPNSQSELKRLEYRTRWEDDFIDYLTKLDNHKPVIVCGDMNVAHKEIDLKNPKNN
                     MKNAGFTKEEREKFSKLLDSGFIDTYRYFNPDKEGVYSWWSYRFNARKNNAGWRIDYF
                     CASKKLEDRLVSADIHTEILGSDHCPVELEIK"
     gene            complement(1804..2754)
                     /gene="galT"
                     /locus_tag="PC471_003796"
     CDS             complement(1804..2754)
                     /gene="galT"
                     /locus_tag="PC471_003796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HDF2790872.1"
                     /translation="MKELRIDPITNDVVIFATDRLKRPLDTADIPNEDEETNEYDEEC
                     PFCKGNEAHATDALFEIDSEEGWLVKSVYNKFPIIDDMARDVYGVHEVMIESDKHNRS
                     FYNMSQKEFEDVFFMYRNRFRDLSKDDKVEYVSIFKNFLRKAGASLMHPHAQILSMSF
                     IPPEITNELLVSKEYYDSNKSSLYDDLIENEISLDRRVVYNGEAFLVLIPYATKYSGE
                     VRIIFKDKIEFGELNDNNIQELSTIFEKLFKKLYNINGYMPFNLCIHTHPTKIETKSY
                     FNVHMHIIPRKYNFGGFELGTNMYVSSMSPEDLTKKLKID"
BASE COUNT          984 a          477 c          295 g         1059 t
ORIGIN      
        1 aaaaatttag gagataaact ttatggaata cataattttc gatttggaat ttaaccaagg
       61 atttgataaa aaactcaata aaactgtatc caatgaaaag tgtccttttg aaattataca
      121 aataggagca attaaattgg attctaaatt caatataata gatacgttta atagctatgt
      181 aaaacctaca atttataaag acatacatcc ctttattagt cgtatgacta atattaaaag
      241 ttctgatttt aatgattcgc ctactttccc tgaggtctac aacaatttta taaaatttat
      301 atcatcacaa gaccctatat tatgtgtttg gggagctgga gatttgaagg aattatacag
      361 gaatatcaac tatcacaagc taccatcaaa ttccttacct aaatcttata ttaatataca
      421 acaacatgca tctaaatatt tcaataatcc agcaggaaaa agcattggcc tacaaaatgc
      481 tatttctata ttggaacttg atgaaaaaat gtcttatcac aatgctttaa atgatgcata
      541 ttatactgct aaagttttta ttaagatata caatccaagt atagtcccag atatatatct
      601 ttatacgagt ataaaaccta aaactataag atattctaac aaaaaaagag ttgattatga
      661 taagttattt gatgaattta ggaaaatttt aaatcgtgaa ttaactaaag acgagaaaaa
      721 aatcattaac ttagcttata atatgtggaa aacaaatcaa tttacattag aaaatgttaa
      781 gcaaagaaaa aacaaataga gtttatttag ataaaaaata ggcatgtaga ttaaaaaact
      841 agttaacttt aaatttatat ctacagccca atttttatca taattaattt ttattttatc
      901 tctaattcaa ctggacaatg gtctgaacct aatatttcag tgtgtatatc cgcacttact
      961 aatctatctt ctaatttttt ggatgcacaa aaataatcta ttctccaccc tgcattattt
     1021 tttcttgcat taaatctata cgaccaccaa ctataaacac cttctttatc aggattaaaa
     1081 tatctatatg tgtctataaa cccactgtct aataatttag aaaacttctc tctttcttct
     1141 ttagtaaaac ctgcattttt catattattt ttaggatttt ttaaatctat ttctttatgt
     1201 gcaacgttca tatctccaca cacaattaca ggcttatgat tatctagttt ggttaaataa
     1261 tctataaagt cgtcttccca tctagttcta tattctagtc tctttaattc agattgagaa
     1321 tttggcgtat atactgtaac aaagtaaaaa tcttcaaact ctagagttat cactctaccc
     1381 tctttgtcat gttcttctat atttattcca tacattactt ttaaaggctc tttttttgta
     1441 aatatagcag tacctgaata accctttctc tcagcataat tccaatattg aaaatatcct
     1501 ggtaaatcta actctatttg cccttcttgc aatttagttt cttgtaaaca aaatatatct
     1561 gcatctactt ctttgaaaaa gtctaaaaat cctttcccaa cacaagctct tattccatta
     1621 acattccatg atataaattt catacttata ctcctcatct aaacattgac acactcccca
     1681 tagctaaaga tgggggcttt tcaacaattt tattgtaaat tcttaattta cttttatttt
     1741 aacaatgcta tactataatt tcatctaaaa ttactattta cttaatacct aaaaatatat
     1801 gttttaatca atttttaact tttttgttaa atcttcaggg ctcattgaag aaacatacat
     1861 atttgttcca agttcaaatc ctccaaaatt atattttctt gggattatgt gcatatgaac
     1921 attaaaatac gattttgttt caatctttgt tggatgagtg tgaatacata aattaaatgg
     1981 catatatcca ttgatattgt ataacttctt aaacaacttt tcaaatattg tagaaagctc
     2041 ctgtatatta ttatcattta actcaccaaa ctctatttta tctttaaata ttattcttac
     2101 ttctccacta tacttggttg catatggtat aagtaccaaa aaagcttctc cattataaac
     2161 aactcttcta tctaaactta tttcattttc tattaaatca tcatacaaag agcttttatt
     2221 gctgtcataa tactcctttg aaaccaacag ttcattcgta atttcaggtg gaataaaaga
     2281 catagacaat atttgagcat gtggatgcat caaagatgct cctgcctttc tcaagaagtt
     2341 tttgaagata ctaacatact ctactttatc atctttcgac aaatctctaa atctatttct
     2401 atacataaaa aaaacatctt caaattcttt ttgactcatg ttataaaaac tacgattatg
     2461 tttatcgctc tctatcatta cttcatgaac accatataca tctcttgcca tatcatcaat
     2521 tataggaaat ttattgtaga cagattttac aagccatcct tcttcactat ctatttcaaa
     2581 taatgcatca gttgcatgag cttcatttcc tttgcaaaat gggcattctt catcatattc
     2641 attagtttct tcatcttcat ttgggatatc tgctgtatct aaaggtcttt ttaacctatc
     2701 tgtagcaaat ataacaacat cattggtaat tggatctatt cttaattctt tcataattaa
     2761 cacctcttag ttaatagcct ttaatataat tatatgatat ttatcgtatc tgtta
//
LOCUS       DAMJYR010000246         2796 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.251, whole genome shotgun sequence.
ACCESSION   DAMJYR010000246 DAMJYR010000000
VERSION     DAMJYR010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2796)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2796)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2796
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.251"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            347..2158
                     /locus_tag="PC471_003797"
     CDS             347..2158
                     /locus_tag="PC471_003797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HDF2790873.1"
                     /translation="MKKKYDFLIYSAIWLVIIILFIFSLYHSIEHIKIISHTGTIRSE
                     TQKVVKQELNNERNDDLIKRLDNILIKLRTGNGENGFQRCDNKEFQQKLNQMDSMWES
                     MKKEIIKVRNGASGDKLYKLSEEYSVLSNQIVFISEKHSNAKLYSFATALFIYLIFST
                     ISLLIWEYYNKKRFKRIFYTDNLTKIKNQVAFENRAIEILHNASNKEYVLLNIDIDNF
                     KYINDTHGYEYGDKVLIIVATVLSKTFNIKETCARICSDNFVILAKYRDSLLEDIREM
                     LTNAIISELDMNVTQTISYCIGAYLVEIDNLGYKSINSMMDKANIAHKVSKTRGISST
                     VWYNENLLKQLQMENSIYNYMYKAIENEEFHMYLQPKFQISSLNVVSAEALVRWFSPE
                     LGFLSPDEFIPLFEKSGFIIELDFYMLKKACSFVKKTFMKKNQYTYPIAVNFSRVTIY
                     QNSFYQRFLDTVKEYEIPFKYIEIEVTESAFNEISQPVISILEELKKLGFLISMDDFG
                     SGYSSLSLLCSLSINGLKLDKSLLKETFNREKVYSIIQCIIEMSHRIGMSVVCEGIET
                     KKDLEFLNTVKCDVGQGFYFSKPIEEKEFFNKYVTKK"
     gene            complement(2246..>2796)
                     /locus_tag="PC471_003798"
     CDS             complement(2246..>2796)
                     /locus_tag="PC471_003798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2790874.1"
                     /translation="RSYDIGIFKNEQSIYKFIEKIPFVKKDSSNFNYTNYYMEFEAIP
                     EYYEVNFNNYLYIITRFSFIPDGTEIFFTWEEIHCWDEKVATEKTFINGETLVDSYNF
                     PNNEVKDYIQKRESLYIETKKYYEEKGLKIERHALGSEDGEYVELTNDSILYLLDIEA
                     VEIREQSKNIEEFLYKYKKRFK"
BASE COUNT         1050 a          290 c          401 g         1055 t
ORIGIN      
        1 tatttattaa aatatagatt atgtatatgc caaatttaaa aagagacata aagacattat
       61 acaaatatat tattggggtg atattttgta agaaaagaag tgatgcagta aattaattga
      121 gtaatttaaa aatgatttaa tgataatgtt gatgaatgtt taaaaagaaa ttttagaaaa
      181 acgtatattg ctgtttgtgt tggaggtgat gaatttgtag aatttatagg gtataaatga
      241 ttaaactatt taagataaat acatatcact gtaaaatatt gataatcatt ttttataaga
      301 gattgattat ttgcaaatta tatatattac aaatgaggta acaataatga agaaaaaata
      361 tgatttttta atttattctg ctatttggct agttattatt attcttttta tattttctct
      421 atatcatagt atagagcata ttaaaattat aagtcacact ggtactattc gcagtgaaac
      481 tcaaaaggtt gttaagcaag aattgaataa tgaaagaaat gatgacttga taaaacgact
      541 ggataatatt ctgataaagc taagaacagg taatggagaa aatggatttc aaagatgtga
      601 taataaagaa tttcaacaaa aactaaatca aatggattct atgtgggaat ctatgaagaa
      661 agaaattata aaagttagaa atggtgcttc aggtgataaa ttatataaat tgagtgaaga
      721 gtattctgtg ttgtctaatc aaattgtttt tatatctgaa aaacactcta atgcaaaatt
      781 gtattctttt gctactgcac tatttattta tcttatattc tcaacaataa gtcttttaat
      841 ttgggaatat tataataaaa aaaggtttaa aagaatattt tatactgata atttaacaaa
      901 aataaaaaat caggttgcat ttgaaaacag agcgattgaa attttgcaca atgcatctaa
      961 caaagagtat gtactgctaa atattgatat tgacaatttt aaatatatta atgatacaca
     1021 tggatatgaa tatggagata aagttctaat aatagttgca actgttcttt ctaaaacttt
     1081 taatataaaa gaaacttgtg caagaatttg ttcagataat tttgtaatac ttgcaaaata
     1141 tagagattct cttttagagg atattcggga aatgctaact aatgctataa tctctgaatt
     1201 agatatgaat gtaactcaaa ctatatctta ttgtatagga gcttacttag ttgaaattga
     1261 taatttaggt tataaatcta tcaattctat gatggataag gcaaatattg cacacaaggt
     1321 aagtaaaaca agaggaattt cctccacagt atggtataat gaaaatctgt taaaacagtt
     1381 acaaatggaa aatagtattt ataattatat gtataaagcg atagaaaatg aagaatttca
     1441 tatgtatttg caacctaaat ttcaaatatc ttctttaaat gttgtgagcg cagaagctct
     1501 tgtaagatgg ttttctccag aacttggttt tttatcacca gatgaattta ttccattgtt
     1561 tgaaaaaagt ggatttatta ttgaattgga cttttatatg ttaaaaaaag cgtgttcatt
     1621 tgtaaaaaaa acgtttatga aaaaaaatca atatacatat ccgattgcag ttaatttttc
     1681 aagggttact atttaccaaa atagttttta tcaaaggttt ttagatactg ttaaagaata
     1741 tgaaattcct tttaagtata tagagataga ggtaactgaa agtgctttta atgaaatatc
     1801 tcaaccagtt atttcaattt tagaggaact taaaaagtta ggatttttaa tttctatgga
     1861 tgattttgga tcaggatact cttcattaag tcttttatgt tctctatcta ttaatggatt
     1921 gaagttagat aaaagcttac ttaaagaaac atttaaccgg gagaaagtat atagtattat
     1981 tcagtgtatt attgaaatgt cacatcgtat aggcatgtct gttgtatgtg aaggtattga
     2041 aactaaaaaa gacttggaat ttttaaatac agttaaatgt gatgtaggac aagggtttta
     2101 tttttctaaa ccgatagaag aaaaagaatt ttttaacaaa tatgtaacta aaaaataata
     2161 taattatttt tatcagtcta tacattgagt cattttatat ctcgttaaat gactcaatgc
     2221 actgttatgt attttaaaat ataaattatt taaaacgttt tttatactta tatagaaatt
     2281 cttctatatt ttttgattgt tctcttattt caactgcttc tatatctagt aaataaagta
     2341 tagaatcatt tgtaagttca acatattcac catcttctga accgagtgca tgtctctcaa
     2401 tttttaatcc tttttcttca taatattttt ttgtttctat atataatgat tctcgctttt
     2461 gtatgtagtc tttcacttca ttatttggaa agttatatga atccactaat gtttcgccat
     2521 ttataaaagt tttctcggta gcaacttttt catcccaaca atggatttct tcccaagtaa
     2581 aaaatatctc agttccatct ggaatgaatg aaaacctggt aataatatat aaataattat
     2641 tgaaatttac ttcataatat tcaggaatag cttcaaattc catatagtaa tttgtataat
     2701 taaagttact actatctttt tttacaaatg gtattttctc aataaattta tatattgatt
     2761 gttcattttt aaaaattcct atatcatagc ttcttt
//
LOCUS       DAMJYR010000247         2776 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.252, whole genome shotgun sequence.
ACCESSION   DAMJYR010000247 DAMJYR010000000
VERSION     DAMJYR010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2776)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2776)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2776
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.252"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            <1..438
                     /locus_tag="PC471_003799"
     CDS             <1..438
                     /locus_tag="PC471_003799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HDF2790875.1"
                     /translation="EKNIIIFGQGVNPESQNTGDKSGVALKFLYSLLDLKCSKTEKKF
                     KKAIRELLWFVCEYLKISGSKSYDYKSVQITFNHSMIINESEKIDMAAKSIGIISDET
                     IVSNHPWVEDVNDELERLKKQEDTQKEYDDLIPNNQDGVIDET"
     gene            428..1963
                     /locus_tag="PC471_003800"
     CDS             428..1963
                     /locus_tag="PC471_003800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HDF2790876.1"
                     /translation="MKHKDYWRKRFEQLEEAQNNKSVKYYLELEKQYKLAINNIEKDI
                     LAWYNRFAKNEGISLLEAKKLLNTRELEEFKWSVEEYIKYGKENAINQKWMKELENAS
                     ARVHITRLEALKLQIQQQVEVLYGNELDGIDKLMRDIYTSGYYHTAFNVQQGVNVGWS
                     LMSLDTNRINKIISKPWATDGLNFSERIWGKHRHALVNELHTKLTQSIIRGENPKNLV
                     NDFAKRFNVSKSQAKNLIMTESAFFASASRKDCFNDLDVEKYEIIATLDLRTSNICRE
                     LDGKVFDMKDYQVGITAPPFHCRCRTTTAPWFEDEEGYRAAKGEDGKTYYVPSNMKYN
                     EWYEKHIEGRLGKEKADTLRKMQLNESKDRKQFEEYKKVLGNEIPSKFDEYQHMKYND
                     TIRYEEAKKLYKDVNWQVKNQRNLTSGSVHSVPFESKPNSVFDNYKDGKLIQRRYYGN
                     TGKPRLDLDLTDHRNPKQHKIVPHKHDWLADENNHDKVKREKDMELTKAHKIANKDIL
                     KGE"
     gene            1965..2213
                     /locus_tag="PC471_003801"
     CDS             1965..2213
                     /locus_tag="PC471_003801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2790877.1"
                     /translation="MDNKFKNLQHLIDSIDLGLDIEFDLYNKPYNISIGDDDTRFITL
                     CPNGDTKYYKNGKDMVDNYNIDGKLLKDLWKDIQIVNM"
     gene            2283..>2776
                     /locus_tag="PC471_003802"
     CDS             2283..>2776
                     /locus_tag="PC471_003802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage scaffolding protein"
                     /protein_id="HDF2790878.1"
                     /translation="MDWLKELLEGIKIENNKIDVVSLQKSIEKKIKETTITQEDYTNL
                     ETQLNTANEAIKKFEGGMTKEDVENLKTTYETDKKTLEETYKKEIEEKDFNYWLNDAF
                     KSIKCRDEIALKAHLDIEALRNSKDRQKAFEEQINPLKQDKDYLFNATLEGEEPKIDT
                     ITPG"
BASE COUNT         1209 a          284 c          500 g          783 t
ORIGIN      
        1 gaaaagaata taattatctt tggtcaagga gttaatccag aatctcaaaa cacaggtgac
       61 aaatctggtg tagcacttaa atttttatat tcactacttg atttaaaatg ctccaaaact
      121 gaaaagaagt ttaaaaaagc aattagagaa cttttgtggt ttgtatgtga gtatttaaag
      181 ataagtggta gtaaaagtta tgattataaa tcagttcaaa ttacttttaa ccactcaatg
      241 ataataaatg agtctgaaaa aatagatatg gcagctaaat caattggaat catatcagat
      301 gaaactattg tttccaatca tccttgggtt gaggatgtta atgacgaact tgagagactt
      361 aaaaaacagg aagatactca aaaagagtat gatgatttaa ttcctaataa tcaagatggt
      421 gttatagatg aaacataaag attattggag aaagagattt gaacaattag aagaagctca
      481 aaataacaaa agtgtaaaat attatcttga attagaaaag caatataaac tagctataaa
      541 taatatagaa aaagatatat tagcatggta caacagattt gccaaaaatg aaggaatatc
      601 tttattagaa gctaagaaac tactaaatac aagagaacta gaagagttta aatggagtgt
      661 agaagaatat attaaatatg gtaaagaaaa tgctataaat caaaagtgga tgaaagagtt
      721 agaaaatgct agtgcaagag ttcatataac aaggcttgaa gctttaaagt tacaaataca
      781 gcaacaagta gaagttttat atggaaatga acttgatggt attgataaac taatgagaga
      841 tatttataca agtggatact atcatacagc ttttaatgtt caacaaggag taaacgttgg
      901 ttggagttta atgagtcttg atactaatag aataaataaa attatctcta aaccatgggc
      961 aacagatgga ttaaacttta gtgaaagaat ttggggtaag catagacatg ctttagtaaa
     1021 tgaactacat actaagctaa ctcaatcaat tattagaggt gaaaatccaa agaatctagt
     1081 aaatgacttt gctaaaagat ttaatgtatc taaatctcaa gctaagaact taataatgac
     1141 tgaatcagct ttctttgcat cagcaagtag aaaagattgt tttaatgatt tagatgtaga
     1201 gaaatatgag ataattgcta cactagattt aagaacttca aatatatgca gagaattaga
     1261 tggtaaagta tttgatatga aagattatca agttggaata acagctccac catttcattg
     1321 tcgttgtagg acaacaacag ctccttggtt tgaagatgaa gaaggttaca gagcagcaaa
     1381 aggagaagat ggaaaaacat attatgtacc atctaatatg aagtataatg agtggtatga
     1441 gaaacatata gagggtagac ttggaaaaga aaaagctgat acccttagaa aaatgcaact
     1501 aaatgagagt aaagatagaa aacaatttga agaatataaa aaggtattag gaaatgaaat
     1561 accttctaaa tttgatgaat atcaacatat gaagtataat gatactataa ggtatgagga
     1621 agctaaaaag ctttataagg atgtaaattg gcaagttaag aatcagagaa atttaactag
     1681 tggaagtgtt cattcagtgc catttgaatc aaaaccaaat agtgtatttg ataattacaa
     1741 agatggtaaa ttaattcaaa gaagatacta tggtaataca ggaaaaccta gattagattt
     1801 agacttaact gaccatagaa acccaaagca acataaaatt gtacctcata aacatgattg
     1861 gttagctgat gaaaataacc atgataaggt aaaaagagag aaggatatgg agcttacaaa
     1921 agctcataaa atagctaaca aagatatttt gaaaggagag taaaatggat aataaattta
     1981 agaatttaca acatttaatt gattctattg atttaggatt agatatagaa tttgacttat
     2041 ataataaacc atataatata tctattggag atgatgatac aaggtttatt actctttgcc
     2101 ctaatggaga tacaaagtat tataaaaatg gtaaggatat ggttgataat tacaacattg
     2161 atggtaaatt actaaaggat ttatggaaag atatacaaat agtaaatatg taaaagcact
     2221 tgctaaatga taaattagta ggtgctttta ttatgtaaaa aatgaaagga gatatttaaa
     2281 agatggattg gttaaaagaa ttgctagaag gaataaaaat agaaaataac aaaattgatg
     2341 tagtttctct tcaaaaatct atagaaaaga aaataaaaga gactacaatt actcaagaag
     2401 attatacaaa tcttgaaaca caacttaata cagctaatga agctattaaa aagtttgaag
     2461 gaggtatgac aaaagaagat gtagagaatc taaaaacaac ttatgaaact gataagaaaa
     2521 ctttggaaga aacctacaaa aaagaaattg aagaaaagga ctttaattat tggttaaatg
     2581 atgcttttaa gtctattaaa tgtagggatg aaatagcgtt aaaagctcat ttagatatag
     2641 aagcactaag aaatagtaaa gatagacaaa aagcttttga agagcaaata aaccctttga
     2701 aacaggataa agattatttg tttaatgcaa cactagaagg tgaagagcct aaaatagata
     2761 ctataacacc agggca
//
LOCUS       DAMJYR010000248         2730 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.253, whole genome shotgun sequence.
ACCESSION   DAMJYR010000248 DAMJYR010000000
VERSION     DAMJYR010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2730)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2730)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2730
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.253"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            144..1067
                     /locus_tag="PC471_003803"
     CDS             144..1067
                     /locus_tag="PC471_003803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2520 domain-containing protein"
                     /protein_id="HDF2790879.1"
                     /translation="MGSGKVGTSLANYFLSKGYCVSGFYGRNQLSLLESTNLTKTKIY
                     SNLKDIIYENDILFITTSDDSIEIIDKKLSKFNLKHKYICHTSGSLKSSILFCSKKAG
                     ALIYSIHPIFAFSNKNTDIKKMKDICFSIEGDNEQDDLVIQNFIDDLGNQFFIRDKDT
                     SSTYHLANVLVSNLVLSLLDIGVSYFIKLGLSEEESLKAISPLVKKNIENILDNGFTK
                     ALTGPVSRGDITPIRKHLSVLDKKDEDIYKILSLNLLKIIALGNDNSLKGIKNITIEN
                     AIENLISKSEKYKEIYKLLGGKNDEKYHTDF"
     gene            1042..1869
                     /gene="panB"
                     /locus_tag="PC471_003804"
     CDS             1042..1869
                     /gene="panB"
                     /locus_tag="PC471_003804"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011721175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HDF2790880.1"
                     /translation="MKNTIQTFKNAKYEGKKLSMLTAYDYSIAKIMDECDINGILIGD
                     SLGMVIKGEENTLSVTIDEIIYHTKAVKNGVKNALIVSDMPFLSYHVSIEDAVKNAGR
                     LIKEGGAHAVKLEGGSNVIKQIESIVNAQIPVMGHLGLTPQSVNSFGGFKVQGNTSET
                     ARQLIEDAKLIEKAGAFSIVLEGVPTKIAEMVTNSISIPTIGIGAGINCDGQILVYQD
                     MLGMFGDFVPKFVKQYANIGDIIKDSIKNYILEVNTGAFPQEKHSFSINESELEKLY"
     gene            1888..>2730
                     /locus_tag="PC471_003805"
     CDS             1888..>2730
                     /locus_tag="PC471_003805"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HDF2790881.1"
                     /translation="MLVKEIKLLRNIIKDWRKHGYSIGLVTTMGFLHEGHQSLIKKAV
                     KENDKVVVSIFVNPTQFGPNEDFNSYPRDIDKDFKYCMDSGATVVFNPSPEEMYLKGN
                     CTTINVSGLTDFLCGAKRPVHFGGVCLVVSKFLNIVTPDKAYFGEKDAQQLAVIKRMV
                     KDLNIDTEIIGCPIIRENDGLAKSSRNTYLSEEERKSALILNKSLSLAKEELVKGNLN
                     PENIKELITAKINSEHLAKIDYVEIVDSETLQPVKQIEHSILVAIAVFIGKTRLIDNF
                     TFELN"
BASE COUNT         1084 a          333 c          452 g          861 t
ORIGIN      
        1 tttatggatg gtatcttgtt ataagaacta tactttaaat gtttttaatt attttgtctt
       61 atgtaactca tatcctcagg aatgagatta gaaattaaga tgttgtatca caaagtggag
      121 gtgatactat taaaataggc tttattggtt ctggaaaagt tggaacatct ttggcaaatt
      181 attttttatc aaaaggttat tgtgtttcag gcttttatgg aaggaatcaa ttatctttat
      241 tagaatcaac aaatttaaca aaaacaaaga tttacagcaa tttgaaagat ataatttatg
      301 aaaacgatat attatttatt acaacatcag atgattctat tgaaattata gacaaaaaac
      361 tttccaaatt taatttaaaa cataaataca tttgccacac aagtggctct ttaaaatcta
      421 gtatattgtt ctgttcaaaa aaagcaggtg cattaattta ttctatccat cctatatttg
      481 cattttccaa taaaaatact gacataaaaa aaatgaaaga catttgtttt tccatagaag
      541 gtgataatga acaagatgat ttagttattc aaaatttcat agatgatttg ggaaaccaat
      601 tttttataag agataaggat acttcatcca cttatcattt agctaatgtt ttagtttcaa
      661 atttagtatt atctttacta gatataggag taagttactt tattaaatta ggtttatctg
      721 aagaggaatc tttaaaagct atcagtcctt tagtaaaaaa aaacatagaa aatatacttg
      781 ataatggctt tactaaagct cttactggtc ctgtttctcg tggagatata acaccaataa
      841 ggaaacatct atctgtattg gataaaaaag atgaggatat ttacaaaata ctatccttaa
      901 acctactaaa aataattgcc ttaggaaatg acaattcatt aaaaggtata aaaaatatta
      961 ctatagaaaa tgcaatcgaa aatttaataa gtaaatctga aaaatacaaa gaaatttaca
     1021 aacttttagg aggaaaaaac gatgaaaaat accatacaga cttttaagaa tgctaaatat
     1081 gaaggaaaaa aactatctat gcttactgct tatgactact caattgcaaa aataatggat
     1141 gagtgtgata taaatggtat tttaatagga gattcattag gaatggtcat aaaaggtgaa
     1201 gaaaacactc tatctgttac catagacgaa attatatatc atacaaaagc tgtaaaaaat
     1261 ggcgtaaaaa atgccttaat cgtaagtgat atgccttttc tatcttacca tgtttctata
     1321 gaagatgcag ttaaaaacgc tggtagactt ataaaagaag gaggagctca tgctgtaaag
     1381 ttagaaggtg gctcaaacgt tataaagcaa atagaaagca ttgtaaatgc tcaaatacct
     1441 gtaatggggc atttaggatt aactcctcaa tcagtaaatt catttggtgg gtttaaggtt
     1501 caaggaaata ccagtgaaac agcaagacaa cttattgaag atgctaaatt aatagaaaaa
     1561 gcaggtgcat tctctattgt ccttgaagga gtgcctacga aaatagctga gatggtaact
     1621 aattctattt caattcctac aataggtata ggggcaggaa ttaactgtga tggacaaatt
     1681 ttagtttatc aagatatgtt gggaatgttt ggagattttg ttcctaagtt tgtaaagcag
     1741 tatgcaaata taggagatat aataaaagat tcaattaaaa attacatcct agaagttaat
     1801 acaggtgcat ttccacaaga aaaacacagc ttttcaataa atgaatctga gttagaaaaa
     1861 ttatactaat attaaggatg gtacaaaatg cttgtaaaag aaattaaatt actaagaaat
     1921 ataatcaaag attggagaaa acatggctat tctataggcc ttgttacaac aatgggattt
     1981 ttacatgaag gacaccaaag tcttataaaa aaagctgtaa aagaaaatga taaagtagta
     2041 gtgagtatat ttgtaaatcc aactcaattt ggaccaaatg aagattttaa tagctatcca
     2101 agagatatag ataaagattt taaatattgt atggatagtg gagcaacagt agtctttaac
     2161 ccatcaccag aagaaatgta tttaaaaggt aattgtacta ccattaacgt ttctggactt
     2221 acagattttc tttgtggagc taaaagacct gttcattttg gaggtgtctg cctagttgtt
     2281 tcaaagtttc taaatatagt aacacctgat aaagcttatt ttggagagaa ggatgcccaa
     2341 caattagctg taattaaaag aatggtcaag gatttaaaca tagatacaga aataataggc
     2401 tgtcctataa ttcgtgaaaa tgatggatta gcaaaaagtt cgagaaatac atatctctct
     2461 gaggaagaac gaaaatctgc tctaatatta aataagagct taagtttagc aaaagaagaa
     2521 ttagtaaaag gcaatctaaa tccagagaat ataaaagaat taataacagc taaaataaat
     2581 tctgaacact tagcaaaaat agactatgtt gaaattgtag atagtgagac attacaacct
     2641 gttaagcaaa tagaacattc tatacttgtg gcaattgctg tgtttatagg aaaaaccaga
     2701 ctaattgata attttacatt tgagttaaat
//
LOCUS       DAMJYR010000249         2626 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.254, whole genome shotgun sequence.
ACCESSION   DAMJYR010000249 DAMJYR010000000
VERSION     DAMJYR010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2626)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2626)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2626
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.254"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            303..2438
                     /gene="rnr"
                     /locus_tag="PC471_003806"
     CDS             303..2438
                     /gene="rnr"
                     /locus_tag="PC471_003806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HDF2790882.1"
                     /translation="MIPGLKESLLGLINESAYNPLKKEELAEIFDIHSSEMPMFYNFL
                     NELEEDGYICFTKKGKIVSPNQMGYFVGKFVSHKKGFGFVESDVEYTQDLFISSDNIN
                     GAMHNDRVMAEIVVPATEEKRAEGRIVKVIKREITRIVGTFQSSKTFGFVTPDNKKFT
                     KDIYIPKKHFSGAVDNDKVVCEITVWPQEDRKPEGKIIEILGQKGERGVEIDSIIREH
                     GLPEEFPKKVLQEAEAVAVEIPQEEIKRRRDLRDLNIFTIDGDDAKDLDDAISIEVLP
                     NGNFKLGVHIADVTHYVREKNKLDKEALKRATSVYLVDKVIPMLPKTLSNGVCSLNPF
                     EDKLTLSIFMEIDHKGNVVKHEICESVINSKARMTYTEVSDILEKDDEKLKKTFEHVV
                     EDFKNAEILARILMSRREKRGAIDFNFPEAKIILNGKGEVVDIKPYERRISNKIIEEF
                     MLISNETIAEHFYWMGIPFVYRIHETPSLEKMEELSKFISTFGYIIKGDKEEVHPKAL
                     QGIIEKIKGKKEEGAISTIMLRSLKQAKYSPECSGHFGLAAKFYCHFTSPIRRYPDLQ
                     IHRIIKESLNNKISGKRQEQLTTIVDYASVQSSEKERKAELAERDVHDFYKALYMKDK
                     VGQEFEGVVSSVTSFGIFVELDNTIEGLIRLANMNDDYYIYDESSYSVLGERTKKSYK
                     IGDLVKIKVESVNVDFKEIDFEIVEKIEE"
BASE COUNT         1133 a          257 c          480 g          756 t
ORIGIN      
        1 aatattttac caatcctatt tgatggtgta taattaggtg ataaagagta atttaagcaa
       61 ttatgataaa gaataactta agtaattata atgatattta caaataattt ataaaacatc
      121 ataatttgtt tgaagttagt atgacaaagc ttacattaaa tcaaaaatat aaataagtaa
      181 ttttaactta taataataag aaaatataga aaattatgtg ataattaaaa aatgttttgt
      241 ctgaagaaat ataaaagaca atataaataa taatataatg aaaagtaaaa caggagggaa
      301 aaatgatacc aggacttaaa gaaagtttgc taggattaat aaatgaaagt gcatataatc
      361 ctctaaaaaa agaagaatta gcagaaatat ttgatataca ttcatcagaa atgccaatgt
      421 tttataattt tttaaatgaa ttagaagaag atggttatat ctgctttact aaaaaaggaa
      481 aaatagtatc tcccaatcaa atgggatact ttgttggaaa atttgtatca cacaaaaagg
      541 gttttggatt tgtagaatct gatgttgaat atacacagga tttatttatc tcaagtgaca
      601 atataaatgg tgctatgcat aatgatagag ttatggcaga aatagtagta cctgcaactg
      661 aagaaaagag agcagaagga agaatagtaa aagtaataaa gagagaaata actagaatag
      721 taggtacttt ccaatcaagt aaaacttttg gatttgtaac accagacaac aagaaattta
      781 ctaaagatat atatatacct aaaaaacatt ttagtggagc agtagataat gataaggtag
      841 tatgtgaaat tacagtatgg ccacaagagg atagaaaacc agaaggcaaa attatagaga
      901 tactaggtca aaaaggtgaa agaggcgtag aaatagattc tataataaga gaacatggtc
      961 ttccagaaga atttccaaag aaagtattgc aagaagcaga agctgtagct gttgagattc
     1021 ctcaagaaga gataaaaaga agaagagatt taagagattt aaacatattt actatagatg
     1081 gagatgacgc aaaagactta gatgatgcca tatcaataga agtattacct aatggaaact
     1141 tcaaactagg agttcacata gctgatgtta cacattatgt aagagagaaa aataaacttg
     1201 acaaggaagc cttaaaaaga gctacatcag tttatttagt agataaagta attccaatgt
     1261 tgccaaaaac tctttcaaat ggagtatgta gcttaaatcc atttgaagat aaattaactc
     1321 tttctatatt tatggaaata gaccataaag gaaatgtggt taaacatgaa atatgtgaat
     1381 ctgtaataaa ttcaaaagct agaatgactt atacagaagt atctgatata ttagaaaagg
     1441 atgatgaaaa acttaaaaag acttttgaac atgttgttga agattttaaa aatgctgaaa
     1501 ttttagctag gattcttatg tcaagaaggg aaaaaagagg agctatagac ttcaatttcc
     1561 cagaggcaaa gataattcta aatggaaaag gcgaagttgt agatataaag ccatatgaaa
     1621 gaagaatatc taataagata atagaagaat ttatgcttat aagtaatgag acaattgccg
     1681 aacattttta ctggatggga ataccgtttg tgtatagaat acatgagaca ccatctttgg
     1741 aaaaaatgga agaattgagt aagtttatat ctacatttgg atatataata aaaggtgata
     1801 aagaggaagt acatccaaaa gcattacaag gtataataga gaaaataaag ggcaaaaaag
     1861 aagaaggagc tatcagtact ataatgcttc gttctttaaa acaagctaag tactcaccag
     1921 aatgtagtgg acattttgga ttagcagcta aattctactg tcactttact tcaccaataa
     1981 gaagatatcc tgaccttcaa attcatagaa taataaaaga atctttaaat aataaaataa
     2041 gtggaaaaag acaagaacag cttactacta tagtggatta tgcatctgtt caatcatccg
     2101 agaaagaaag aaaggcagag ttagctgaaa gagatgtaca tgatttctat aaggctcttt
     2161 atatgaagga taaagttggt caagaatttg agggtgttgt atctagcgta acttcatttg
     2221 gaatatttgt tgagttggat aataccatag aaggattgat aagacttgca aatatgaatg
     2281 atgattacta tatttatgat gagagttctt atagtgtttt aggagaaaga actaagaagt
     2341 catataagat aggagacctt gttaagataa aggttgaaag tgttaatgta gattttaaag
     2401 aaattgactt tgagatagtt gaaaaaatag aggagtagat aaaacagcta aaataagaga
     2461 taacaagttg tagtatttat gatatgtata aatatatagt gcacaatact ctataaaata
     2521 aatgataaaa ataggtaatt gagaaatatc taatggaaat agttaactga aagataaata
     2581 attaagaaat atttaatgaa agtagctaat tgaaaaataa gtaatt
//
LOCUS       DAMJYR010000250         2605 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.255, whole genome shotgun sequence.
ACCESSION   DAMJYR010000250 DAMJYR010000000
VERSION     DAMJYR010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2605)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2605)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2605
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.255"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(226..1476)
                     /locus_tag="PC471_003807"
     CDS             complement(226..1476)
                     /locus_tag="PC471_003807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 domain-containing protein"
                     /protein_id="HDF2790883.1"
                     /translation="MATIKPFKAIRPNKYIVDKVAALPYDVMNSKEARRIAEGNPYSF
                     LHIDKSEIDLDENIDLYDEKVYLKARENLDEFRKQEILVKDDKECIYIYKQIMDGRAQ
                     VGIVACISVDESLNGTIKKHEYTRPEKEIDRTKHIKYCDANTGTILVTYKHQRVIDDI
                     INDFMDNNEPLYDFITDDKIEHTVWKIDNDDIIDNLVDKFEKLDYLYIADGHHRTASA
                     ENVAKEMRAKNPNYTGKEEFNYFIAMIAPDENLMVLDYNRVIKDLNGLSEEEFINKIK
                     ENFELEEIEGKEKYKPDKKGTFGMYLGDKWYKMKANKNLLEIEDPVDSLDISILQDYV
                     IDAILGIDNPRVDKRIDFIGGIRGLEELEKRVNEDMKVAFAMYPTSIEDLIRVADANK
                     IMPAKSTWFEPKVRCGLFLHEINE"
     gene            complement(1508..2449)
                     /locus_tag="PC471_003808"
     CDS             complement(1508..2449)
                     /locus_tag="PC471_003808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-3-phosphoglycerate dehydrogenase"
                     /protein_id="HDF2790884.1"
                     /translation="MYNILVTDGIEKEAARKLRELDFNVIEQFYEKDVLGDKLKDVDV
                     LVVRSATKVTKDVIDKALEGKKLKLIVRGGVGLDNIDVKYAQANGIKVMNTPNASSIS
                     VAELTIGQLFVLARFINTANVTMRDGKWEKKKYKGTEINGKTLGLIGFGRIAKEVAKR
                     AELLGMNVIYTDIMGEAQGFNNYKFCDMEEVLENADFLSLHIPFDKNKGAVITEKEIN
                     KMKKGAYLINCARGGLVDEKDLLKALDEGKLSAAAIDVYEQEPTLNLDLVNHPRVSPT
                     PHIGASTVEAQERIGDEIVNVIQDFFLDFNNLIGVAL"
BASE COUNT          783 a          497 c          243 g         1082 t
ORIGIN      
        1 aatacacatt taaaacctta taaatataca aatacaatta tatgaatttt catcctcaat
       61 tatagcatag aatatgacta tctatatcct aaaattatcc atttctaaaa aaaaggtaac
      121 tattattaag aatatcaact tatagaattt atattccaca tacgattttc aatcaaaaaa
      181 acaagggcta aaaagtgaat tgtgcacttt ttagcccttt attaattatt catttatttc
      241 atgtaagaat agaccacatc ttactttagg ttcaaaccat gttgacttag ctggcattat
      301 cttatttgca tctgctactc ttattaaatc ttcaatacta gtaggataca ttgcaaatgc
      361 tactttcata tcttcattaa ctcttttttc taattcttca agacctctaa ttccaccgat
      421 aaaatcaatt cttttatcta ctctaggatt atcaattcct aaaatagcat caataacata
      481 gtcttgtaat atagaaatgt ctaatgagtc aacagggtct tctatttcta ataaattttt
      541 atttgctttc attttatacc acttatcacc taggtacatt ccgaaagttc cttttttgtc
      601 aggtttatat ttttcttttc cttctatttc ttctagttca aaattttctt ttattttatt
      661 tataaattct tcctcgctta aaccatttaa gtctttgata accctattat aatccaaaac
      721 cattaaattc tcatctggtg caatcattgc tatgaagtaa ttaaattctt cttttccagt
      781 ataatttgga tttttagctc tcatttcttt ggctacattt tctgctgaag ctgtcctatg
      841 atgaccatct gcaatgtata gataatctaa tttttcaaat ttatctacta ggttatctat
      901 tatatcatca ttatcaatct tccatactgt atgctctatc ttgtcatctg ttatgaaatc
      961 atataaaggt tcattattat ccataaaatc attgattata tcatctatca ccctttgatg
     1021 tttgtaagtt acaagtatag ttcccgtatt agcatcacag tattttatgt gtttagttct
     1081 atcaatttcc ttttctggtc gagtgtactc atgttttttt attgttccat tcaagctttc
     1141 atcaactgat atacatgcta ctataccaac ttgagctcta ccatccatga tttgtttata
     1201 tatatatata cactctttgt catcttttac tagaatttct tgttttcgaa actcatctaa
     1261 attttctcgc gcttttaaat aaactttttc atcatataaa tctatatttt catctaaatc
     1321 aatttctgat ttatctatat gcaaaaatga atatggattc ccttctgcaa ttcttctagc
     1381 ttctttacta ttcataacat cgtaaggaag tgctgctact ttatctacta tatatttatt
     1441 tggtctaatc gccttaaatg gtttaatcgt tgccattact aatttcctcc taattataca
     1501 aaatatttta taatgctacc cctattagat tgttgaagtc aagaaagaaa tcttgaatca
     1561 catttactat ttcgtctcct attctttctt gagcttctac tgtagatgcc ccaatatgag
     1621 gtgtaggaga aactcttgga tgatttacta agtctaaatt taatgtaggt tcttgttcat
     1681 aaacatctat agcagctgct gataatttac cttcatctaa tgcttttagt aaatcttttt
     1741 catcaactaa accgcctctt gcacagttta ttaaataagc ccctttcttc atcttattta
     1801 tttctttttc agttattact gcccccttat tcttatcaaa tggtatgtgt aaagataaga
     1861 aatctgcatt ttctaaaact tcttccatat cacagaattt ataattgtta aagccttgcg
     1921 cctcacccat tatgtcagta tatataacat tcatacctaa taattctgct ctctttgcaa
     1981 cttcttttgc aattcttcca aaacctataa gtccaagtgt ctttccatta atttctgtac
     2041 ctttatactt tttcttctcc cattttccat ctctcatagt tacatttgca gtatttatga
     2101 atcttgcaag aacaaataat tgacctattg taagctctgc aacagatata ctgcttgcat
     2161 ttggagtatt cataactttt atgccattag cttgagcata tttaacatct atattatcca
     2221 gacctactcc acctcttact atcaacttca actttttacc ctctaatgct ttatctatta
     2281 catctttagt aactttagta gctgaacgta ctacaagtac atcaacatct ttaagtttat
     2341 ctcctaaaac atctttttca taaaactgct ctatcacatt aaaatctaac tctctcaatt
     2401 ttctcgctgc ttctttctct attccatcag taactaatat attatacata acaaactcct
     2461 ccaattttta ttcacttaat ttttccaatt tttatttttt aaagtatttt catcaataaa
     2521 aaactttact taatttaact ttctgtttat ataattttca aaatattttt ttattaaagt
     2581 catatcaaat ttttctattg tttgt
//
LOCUS       DAMJYR010000251         2605 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.256, whole genome shotgun sequence.
ACCESSION   DAMJYR010000251 DAMJYR010000000
VERSION     DAMJYR010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2605)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2605)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2605
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.256"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            498..1319
                     /locus_tag="PC471_003809"
     CDS             498..1319
                     /locus_tag="PC471_003809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002572585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallohydrolase"
                     /protein_id="HDF2790885.1"
                     /translation="MKLKIVGSGGMSIIPSSFCKCKICEEARQKGGRYERLGPSLYID
                     DIKMLIDTPEDIAVACNRQKISEVKHISISHHDPDHVKGIRIVEKIGCDFITGENNPI
                     GFYALPEVIEDINRTNLDCLKYYGDVINCISINETSHVKINNFDIDLINNNPDRNITF
                     YVIKENEKKVIYACCNPKPFTHSDMYFDADILIISLVSDDGILNDGTKLKDTPFKDEI
                     FTLDEIVEIKNYYRIKKIIVTHIDEMWGKSYGYYSELEKKLDNIYFAYDGMEIIV"
     gene            complement(1629..2408)
                     /locus_tag="PC471_003810"
     CDS             complement(1629..2408)
                     /locus_tag="PC471_003810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HDF2790886.1"
                     /translation="MSYKKIAIITGANSGFGKEFLKLLINEEEITEIWAVARNKARLN
                     QLVDEFGSKVKIFSKDLSKIEEVKEIGTFLSKENVCIKYLINNAGFAKFCSYNDLSID
                     ESINMIDLNISAVVALGLICIPYMQKGSHIMNISSQASFQPLPYQNIYSSTKAFIRNY
                     TRALNVELKSKGINAIAVCPGWMSTNLFKRGIVVAEKGTKNFPGIVSPDVVAKKALKD
                     AKKNKDISVYGINTKSSHLLAKLLPQKMMMKVWLMQQHIKE"
BASE COUNT          955 a          374 c          305 g          971 t
ORIGIN      
        1 aattataaga ttatatacta tccttatact ctagttaaat atattttaat cactaatata
       61 aattagtttt tttaacaaaa caataaaaga gtaaagtaca aataattcct ttgtactcta
      121 ctctttttaa ctttctttgt acaataatac aatgataaat ataaaaaatt atatattcca
      181 ttctatagaa atatgctcac caataacatg tatgttgtta atcaatatat tatatcaaat
      241 tatatcaaaa ttacataaac cttataggac aatcatatct tattacaaga aaaatgtctt
      301 atccatattg aataagtcat tcagttcata tacaaagtca atttcattga gaaacattta
      361 atatatgttt ataaaaattt catactattc tttcatattt acatgtgata taatttatta
      421 actcataatt ttgttaatca gttgtgcttg tatattattt atataatcta atatttttag
      481 aaggtggtgt aataactttg aaattaaaaa ttgttggtag tggcggtatg tcaattattc
      541 caagttcatt ttgtaaatgc aaaatatgtg aagaagccag gcaaaaaggc ggaagatatg
      601 aacgattagg accaagttta tatattgatg atataaaaat gctgatagac acacctgaag
      661 acattgcagt agcttgtaac agacaaaaaa tatcggaagt aaaacatatt tcaatttctc
      721 accatgaccc agaccatgtt aagggtatta gaattgtcga aaaaatagga tgtgacttta
      781 tcacaggaga aaataatcca attggctttt atgcgcttcc tgaagtaatt gaagatataa
      841 accgaacgaa tctcgattgt cttaaatatt atggtgatgt tataaattgc atttctatta
      901 atgaaacttc gcatgtaaag ataaataatt ttgatattga tttgattaac aataacccag
      961 acaggaatat aaccttctat gttatcaaag agaatgaaaa aaaagtcata tatgcctgct
     1021 gtaatcccaa gccatttact catagtgata tgtactttga tgctgatatt ttaataataa
     1081 gtctagtttc tgatgatggt atattaaatg atggtacaaa acttaaagac acaccattta
     1141 aagatgaaat atttacttta gatgagattg ttgaaattaa aaactattat agaattaaaa
     1201 aaattattgt tacacatatt gacgaaatgt ggggaaaatc atatggttat tacagtgaat
     1261 tagaaaagaa acttgataac atttattttg catatgatgg tatggaaatt atagtctaaa
     1321 tcattaagta ataagcataa tgcaaaataa gaaaacttca atttaacagt atagtcaaac
     1381 tatgtgatat caacatatcg cagtttttac aaaatatcat agtatttatc tttataaaat
     1441 ttcaagtcct acatatttta atacttcaat atctaaataa agtaaaatca aaaatatatt
     1501 ttatatacat tatagacggc ttatcgtcac tagataagcc gttttgtcac aattattatt
     1561 tgctcaacaa aacttctcat aatatataac agattgaatc tccaactgat acttaattac
     1621 caaaactctt attccttaat atgttgttgc attaaccaaa ccttcatcat cattttttgt
     1681 ggcaatagtt ttgctaataa atgactggat tttgtattta taccatacac agaaatatcc
     1741 ttatttttct ttgcatcctt caatgctttt ttggcaacaa cgtctggaga cactatgcct
     1801 gggaaatttt ttgtcccttt ttcagctaca actatacctc tcttaaatag atttgtactc
     1861 atccaacctg gacatacagc aattgcattt atccctttac tcttaagttc aacatttaat
     1921 gctcttgtat aatttcttat aaacgcttta gttgaactat aaatattttg atatggcaat
     1981 ggctggaatg atgcttgtga agaaatattc atgatatggc ttcccttttg catatatgga
     2041 atacatataa gtcccaatgc tacaacagca ctaatattta aatcaatcat attaatagat
     2101 tcatctatac ttaaatcatt gtatgaacag aattttgcaa aaccagcatt gtttataagg
     2161 tattttatac atacattttc tttacttaaa aatgtaccaa tttcttttac ttcttctatt
     2221 tttgataaat cttttgaaaa aatttttacc ttacttccaa attcatctac taattgattt
     2281 aatctcgcct tgtttcttgc aactgcccat atttctgtta tttcttcttc atttattaat
     2341 aactttagaa actctttacc aaaaccacta ttagcacccg ttataattgc aatcttctta
     2401 taactcataa ttattctcct ttaccataag tttatacgct caaactgtaa acttattttt
     2461 tacaaataaa acaccctcta ttcagatgtt atattcatat tatttgagat attatattta
     2521 tatcattttc aataattgtt atatagcata aaaaatagtg ttaatattat agaatcggta
     2581 ttgattatat ttaaaaagta gtgtt
//
LOCUS       DAMJYR010000252         2563 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.257, whole genome shotgun sequence.
ACCESSION   DAMJYR010000252 DAMJYR010000000
VERSION     DAMJYR010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2563)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2563)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2563
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.257"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            172..1791
                     /locus_tag="PC471_003811"
     CDS             172..1791
                     /locus_tag="PC471_003811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HDF2790887.1"
                     /translation="MNIVISLIGGLGLFLYGMSLMGEGLQKSAGDKLKKIIELLTSNV
                     VMGVLVGTVVTGIIQSSSATTVMVVGFVNAGIMNLSQAIGVIMGANIGTTVTAQLVSF
                     NLEGIAPIALGIGILFYLFTSNQKTKHLSEILIGFGILFTGMEFMKDAVAPLAEYKAF
                     TDALLYFSKNPVLGILAGFAITGIVQSSSASMGMLIALASQGILPLSSALPILYGDNI
                     GTCVTSLLSSVGASRNARRAAVMHLSFNVIGTIIFMLVLNKPISAIVTHFDPTDTARQ
                     IANAHTLFNLTNVIILLPFSKYIVKLANRLIPIKETESEIVNNTKYLDERMFSTPSIA
                     LGNTVQEVVRMGHKATNSLEHSIAGFLNKSNEDINKAFESEKVVNKLQKDILNYLLKL
                     SKEPLRDDERFRTDLLFNTVNDIERVSDHAENIAELAMSVKEMNISFSDSAIREIYEI
                     YNKTITNFKDALIVLDVKDFELANKVLEVENEVNYLEKTFRNSHMIRLNNGSCTIDAG
                     VLYLDLLTNLERISDHSTNIVKQVLKLKQKI"
     gene            2165..2359
                     /locus_tag="PC471_003812"
     CDS             2165..2359
                     /locus_tag="PC471_003812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HDF2790888.1"
                     /translation="MINYMVEIIYSEKISEVENMLKIENIQYEFTSRDGFIFFGFYDM
                     TDAYRFETLCSDEKIRFKRI"
BASE COUNT          952 a          300 c          373 g          938 t
ORIGIN      
        1 aaaattaaca acaacttaaa aatttaacat tctcttaacg tatacttaac aaaacattaa
       61 tatttttatt gacaaaattt gttattctat attatggaaa caataaattt tagttaataa
      121 agtttataat tcagataaaa aaattattaa tttcttaagg gaggaaatat cgtgaatata
      181 gtaattagcc ttatcggtgg acttggactt tttctttacg gaatgagttt aatgggagaa
      241 ggtttacaaa aatcagcagg agacaaatta aaaaaaatta ttgaactctt aacaagtaat
      301 gttgttatgg gtgtactggt aggtacagtc gttacaggta ttatacaaag ttcaagtgct
      361 actactgtta tggttgtagg atttgtaaat gcaggtataa tgaatctaag tcaagctatt
      421 ggtgtaataa tgggggcaaa tataggtact acagttactg ctcaattagt ttcatttaat
      481 cttgaaggaa ttgcaccaat tgctttaggt ataggtattt tattctattt atttacatca
      541 aatcaaaaaa caaagcattt atctgagata cttataggtt tcggtatatt atttacagga
      601 atggagttta tgaaagatgc agtagctcca cttgctgaat ataaagcatt cactgatgca
      661 cttttatatt ttagtaaaaa tcctgtatta ggtatattgg caggatttgc cataactggt
      721 attgttcaaa gttcaagtgc atctatgggt atgttaatag cactagcttc tcaaggaata
      781 cttcctctat catctgcttt acctattctt tatggagata atataggtac ttgtgttact
      841 tcacttttat caagtgttgg tgcaagtaga aatgctcgac gtgcagctgt aatgcattta
      901 agttttaatg taattggtac aataatattt atgcttgttt taaataaacc tatatcagcc
      961 atagttactc attttgaccc aactgataca gctagacaaa tagcaaatgc acatacttta
     1021 tttaatctta caaatgttat aatattatta ccattttcta aatacatagt taaattagca
     1081 aatagactta tacctattaa agaaactgaa tcagaaatag taaataatac taagtactta
     1141 gatgaaagga tgtttagcac tccatctata gcacttggta atactgtaca agaagttgta
     1201 agaatgggtc ataaagctac aaactctttg gaacattcaa ttgcaggatt tttaaataag
     1261 agcaatgaag atattaataa agcttttgag tcagaaaaag ttgtcaataa gttacagaaa
     1321 gatatattga actatttgtt aaaactttct aaagaacctc ttagagatga tgaacgcttt
     1381 agaacagatt tgctttttaa tactgtaaat gacattgaaa gagtctctga ccatgcagaa
     1441 aatatagctg aattggccat gagtgttaag gaaatgaaca ttagtttttc tgatagtgcg
     1501 ataagagaaa tatacgaaat atacaataag acaattacta attttaagga tgccttaata
     1561 gttttagatg ttaaagattt tgagttagca aataaagttt tagaagttga aaatgaagta
     1621 aattatttag aaaaaacttt tagaaattca catatgatta gactaaataa tggaagttgt
     1681 acaattgatg ctggtgtact ttatcttgac ttacttacta atctagaaag aatttctgac
     1741 cactcaacaa atatagtaaa acaagtacta aaacttaaac aaaaaatata gtgattattt
     1801 ttatatataa tcttaatcaa tgagaagtaa gaagctatag ataatgctta tatcatttat
     1861 ggcttcttat tttttatgta atactcaaat ttttatagta ttctcttatt attaaacagt
     1921 ttctgcctag aatattccat atatatatga cttctttcat aaaacttgaa ctgcccataa
     1981 ataaagtcac aagtatacat aactattttt taatcaatta taaccaatat ttgttttatt
     2041 tatcttcatt ttaattcttc attcaataat gtaataatat tattttttat ttggatacta
     2101 ttatatcatc taaatatgtt attatatatt tggatatttt catattaata ggagggattc
     2161 tacaatgata aattatatgg tagaaattat atatagcgaa aaaataagcg aagtagaaaa
     2221 catgttgaaa attgaaaata tacaatatga atttacttct cgtgatggct ttatattttt
     2281 tggtttttat gatatgacag acgcatatag atttgagact ttatgttcag atgaaaaaat
     2341 tcgatttaaa agaatataat agataaaaaa gtacatattt ctaaactatt tgtttttcaa
     2401 atttaggttg tgttttaatg tagtgttgat attataaaca attttaataa taaaaaagta
     2461 gggacattat attaatatgc tcctactttt tactcatact tattaatatt atttatcaaa
     2521 attaccctac aaactggaat ttatcattct ttcataattt taa
//
LOCUS       DAMJYR010000253         2493 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.258, whole genome shotgun sequence.
ACCESSION   DAMJYR010000253 DAMJYR010000000
VERSION     DAMJYR010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2493
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.258"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(<1..754)
                     /locus_tag="PC471_003813"
     CDS             complement(<1..754)
                     /locus_tag="PC471_003813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HDF2790889.1"
                     /translation="MKYKLFIDFEFNILDDNKNKPKEYNGAELISIGGVLVDNEFNTI
                     DNYYSLVKPKYNKILSNKCKNLTKLNQLDINNAPNLLYVMDDFYKWFCKFDDVTIYNW
                     GDFDITGLLTSFRVYKYTGKCLELFNMMIDIQPFISQHITYNNRILSKQISLLNMKKI
                     FSVEGDIKHNSLSDAVDLMNVCKCFFFNSPKNIDILEELYNKLPALKTPLYYIPYFED
                     ENFELKFDRTPEDIIIYLKQILTILNISKKEIY"
     gene            complement(794..1141)
                     /locus_tag="PC471_003814"
     CDS             complement(794..1141)
                     /locus_tag="PC471_003814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2790890.1"
                     /translation="MLNLKEIREEIIKKYIGDSLISELTEELKNILITDLQSNPFKSN
                     IELSIIPANIEIFKKIENILIDSKVNLKMGWERILINYILKDLFELNYEINKSCNEPL
                     MFRVIISIDSYDA"
     gene            complement(1359..2315)
                     /locus_tag="PC471_003815"
     CDS             complement(1359..2315)
                     /locus_tag="PC471_003815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HDF2790891.1"
                     /translation="MKKILITGALGQIGSELTIKLRNEYGEQNVIASSRRVKEGNPVC
                     ESGIFEILDVTDKNRFFEIAKKYDVDTIIHLASLLSAVAESKPLEAWNLNMNGLINGL
                     EIAKELDCKFFTPSSIAAFGENSPKNMTPQDTLQRPNTMYGVTKVSGELLCDYYHSKF
                     GVDTRGVRFPGLISYVTPPGGGTTDYAVDIYYEALKNKRYKSYIAEGTKMDMMYMPDA
                     LQSIVDLIEAPADKLIHRNAFNITAMSFSPEEIADSIKKYIPDFVIEYDVDPVRQSIA
                     DSWPNSLDSSSAVKEWNFKFSYDLDKMTKDMLEKLSEKGIGK"
BASE COUNT          848 a          367 c          287 g          991 t
ORIGIN      
        1 aataaatttc ttttttactt atatttagta tagttaatat ttgtttaaga tatattatta
       61 tatcttctgg agttctatca aatttgagtt caaaattttc atcttcaaag tagggaatat
      121 aatataaagg tgttttgaga gcaggtaatt tattatataa ttcttcaaga atatcgatat
      181 ttttagggga gttaaagaaa aagcatttac atacattcat taaatccaca gcatctgaaa
      241 gtgagttatg tttaatatct ccttctacag aaaaaatttt tttcatattc agtaaagata
      301 tttgcttaga taagattcta ttattgtatg taatatgttg agatataaaa ggttgaatgt
      361 caatcatcat attaaataac tctaagcatt tacctgtata tttatatact ctaaatgaag
      421 ttagaaggcc agttatatca aaatcacccc aattatatat tgtaacatca tcaaatttac
      481 aaaaccattt ataaaaatca tccataacat ataaaagatt aggagcatta ttgatatcta
      541 gttggttcaa tttggttaaa tttttacatt tatttgaaag tattttattg tattttggct
      601 taactaaaga gtaataatta tctattgtat taaattcgtt atcaactaaa actccaccaa
      661 tagaaattaa ttccgcacca ttatattctt ttggtttatt tttattatca tctagtatat
      721 taaattcaaa gtctataaat aacttgtatt tcataaatct aaagcacatc ctcaggtaaa
      781 ttatttattg agcctaagca tcataagaat caatagatat aatgactcta aacatcaaag
      841 gttcattaca acttttattt atttcataat ttaattcaaa taaatctttt aagatataat
      901 ttataagtat tctttcccaa cccattttta aattaacttt agagtctatt aatatatttt
      961 ctatcttttt aaatatttct atatttgctg gtattatact gagctcaata ttacttttaa
     1021 acgggttaga ctgtaaatct gttataagta tattttttaa ttcctctgta agttcagaaa
     1081 ttaaactatc acctatgtat ttttttatga tttcctctct aatttctttt aaatttagca
     1141 atattagtac ctgctactta catagtagct tttcctttcc ttttaatact attattcatt
     1201 tttgaatctt atttagatat attttaagat taaaagtctt tagctatata attttttacc
     1261 catttttaac ttatattaac atattacagg cttatatctc caccctgttt acattgagga
     1321 tggagtatat tgcctatttt gataaaaatg ataggttgct atttgcctat tcctttttct
     1381 gataattttt ctaacatatc tttggtcatt ttatctaaat cataactaaa cttaaagttc
     1441 cattctttta ctgcactact actgtctaga gagttaggcc aagaatcagc tatactttgt
     1501 cttacagggt ctacatcata ttctatcaca aaatcaggaa tatatttttt tatagaatca
     1561 gctatttcct caggagaaaa gctcatagca gtaatattaa aagcatttct atggatgagt
     1621 ttatcagctg gagcttcgat taaatccact attgattgaa gtgcatcagg catatacatc
     1681 atatccattt ttgtaccttc tgctatgtaa gatttatatc tcttattttt taatgcctca
     1741 taatatatat caactgcata atctgtagtt cctccaccag gaggcgtaac ataagaaata
     1801 agacctggaa atctaacacc tctagtgtct actccaaatt ttgagtgata ataatcacat
     1861 agtaattctc cagaaacttt agtcacacca tacatagtat taggtctttg taatgtatct
     1921 tgaggagtca tattttttgg agaattttca ccaaaagcag caattgaact aggtgtaaaa
     1981 aatttacaat ccaattcttt ggctatttca agaccattta ttaatccatt catatttaag
     2041 ttccaagctt ctagaggttt actttcagca actgcagaaa gtaaagatgc tagatgaatt
     2101 atagtatcta catcatattt ttttgcaatt tcaaaaaaac gattcttatc tgtaacatct
     2161 aatatctcaa atataccaga ttcacatact ggattacctt cttttactct ccttgaacta
     2221 gcgattacat tttgttctcc atactcattt ctaagcttta ttgttaattc tgaacctatt
     2281 tgtcctaatg cacctgttat aagtattttt ttcaatttta caacctccta agggtttaat
     2341 ttatgtagta ctacttattt ttattttagc aaatcttttg tttaaataca aagaattaaa
     2401 gataataata tacgacttaa ttgaatgaag tgcagtaagt gcctaaaaat ttaaagctaa
     2461 tatataatgg tatattgtat aacaataaaa gag
//
LOCUS       DAMJYR010000254         2493 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.259, whole genome shotgun sequence.
ACCESSION   DAMJYR010000254 DAMJYR010000000
VERSION     DAMJYR010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2493
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.259"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(188..943)
                     /locus_tag="PC471_003816"
     CDS             complement(188..943)
                     /locus_tag="PC471_003816"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HDF2790892.1"
                     /translation="MLKVIISGYNGTMGRVLAECVKKDNELELVCGVARSADEKGHHS
                     DTKLYKTMTDVTEKTDVIIDFSHHETLEPILSYALKNNTPVVLATTGYDENDLNKIEE
                     ASKNIPILLSSNTSLGVNILIKLVKDASKMLEGFDIEIIEKHHNRKEDAPSGTATMIA
                     NAIREVLPESENTHGRYGRDCKRKPNEIGIHSIRGGNIISDHDVVFAGDNEVLTLSHH
                     SQSDEVFANGSIVAAKFLIGKQSGLYKMSDILG"
     gene            1383..2372
                     /locus_tag="PC471_003817"
     CDS             1383..2372
                     /locus_tag="PC471_003817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HDF2790893.1"
                     /translation="MLKEEKTYQEKDAKVFNGVAALIMILLFLVVSIGIMVFGIVKLD
                     SGFTGIGSLMLILGLVFIVVDFILFFGLKMINPKEAIVLVLFGNYYGTIKKEGYYWVN
                     PFCSAINPAVSRINVSKSSSDEKVDISTNSRGKKVSLKTMTLNNEKQKVNDLLGNPII
                     IGVVVIWKVIDATKAVFNVDNYNTFLSIQCDSTIRNVSRLYPYDVSEDGDEKSLRGSS
                     QEIADRLKDELQSRVDIAGIEVCEVRITHLSYAPEIAAAMLQRQQAEAIIAARKKIVE
                     GAVSMVEMALTNLSENNVVTLDEERKAAMVSNLLVVLCGNKDAQPVVNSSSIY"
BASE COUNT          914 a          310 c          367 g          902 t
ORIGIN      
        1 agattataaa ataataaaca atataattcc atataccaat aaataagata atctttaaga
       61 aaaatgatta tatttaaaag gtaaacataa aatttataaa cttttagaat agaaatctca
      121 atcaattaga tattcaaatt gatgaatatg aatacctaaa tagattgaga tttattatgt
      181 acaaaactta tcctaatata tctgacattt tataaagccc agattgctta ccaattaaaa
      241 atttagctgc aactatagaa ccgtttgcaa atacttcatc agattgagaa tgatggctta
      301 atgtaagaac ttcattatca cctgcaaata caacatcatg gtcgctgatt atattgccac
      361 ctcttataga gtgtattcca atttcatttg gttttctttt acaatctcta ccatatctac
      421 catgtgtatt ttcactttca ggcaagactt ctctaattgc attagctatc atagtagcag
      481 ttccactagg ggcgtcttct tttctattgt gatgtttttc tatgatttca atatcaaaac
      541 cttctaacat ctttgaagca tccttaacta attttattaa tatattcact ccaagagatg
      601 tatttgatga taataatata ggaatgtttt ttgatgcttc ttctatttta tttaagtcat
      661 tttcatcata tccagtagta gctaatacta caggtgtatt attttttaga gcatatgaca
      721 atattggctc taaagtctca tgatgtgaaa agtctattat tacatcagtt ttttcagtta
      781 catcagtcat tgttttgtaa agctttgtat cactatgatg acctttttca tcagcagacc
      841 tagctacacc acaaaccaac tctaattcat tatctttttt tacacattct gctaaaactc
      901 ttcccattgt tccattatag ccactaatta taactttcaa cataaattcc tcctatgaaa
      961 ttaataaaaa tttgttatat ttctataagt ttattttatc actaatataa tttatataaa
     1021 ataatattag tgataataag cttacaattt atcttacatt aaaaaattta agtggatttt
     1081 tcaaaatata gataaatttc tttttcattg tataaatgat aaaaatatat ttgacttcat
     1141 accaaaaaga gtgccccatg aactaaaaag ttaattttga aataccatat tcatatatca
     1201 tatgatttat ttggaaatca atattaatac attaaaataa ctaaatacat gtatgcataa
     1261 agatgtataa aaaatatata aacatatagt attaatttta aattcaacaa tttaattgtt
     1321 aaaagtgtta ttattaaatt agaaatatct taacttaata tactgagatg ggagtgatag
     1381 atatgttaaa agaagaaaaa acttatcaag agaaagacgc aaaagtattc aatggagtgg
     1441 cagctttgat aatgatttta ttatttttag tagtttcaat aggtatcatg gtttttggga
     1501 tagtaaaact tgatagtgga tttactggaa taggttcact tatgttaatt ttgggacttg
     1561 tatttatagt agtagatttt atcctatttt ttgggctaaa aatgattaat cctaaagaag
     1621 ctatagttct agttttgttt ggtaactatt atggaactat aaaaaaagaa gggtactatt
     1681 gggtgaatcc attttgttca gcaataaacc cagcagtatc aagaattaat gtatcgaaat
     1741 cttcatctga tgaaaaggta gatatatcaa ctaattcaag gggcaaaaaa gtatctttaa
     1801 aaacaatgac tcttaataat gaaaagcaga aagtcaatga tttattggga aatccaatta
     1861 taataggagt ggttgtaatt tggaaggtta tagatgcaac aaaggcagta tttaatgtag
     1921 ataattataa tacattctta tctattcagt gtgattctac tattcgcaat gtttcaagac
     1981 tttatccata tgatgtatct gaagatggag atgaaaaatc tcttcgtggt agtagtcagg
     2041 aaatagcaga tagattaaaa gatgaattac aatcaagggt tgatattgca ggaattgaag
     2101 tgtgtgaagt gagaattact catctttctt atgcaccaga gattgcagca gcaatgttac
     2161 aacgtcaaca agcagaggct ataatagcag cacgtaaaaa gattgttgag ggggctgtaa
     2221 gtatggtaga gatggcattg actaatttaa gtgaaaataa tgttgtgact ttggacgaag
     2281 aacgtaaagc agctatggta agtaatctgt tagttgtgct ttgtggcaat aaagatgcac
     2341 aaccagtggt aaacagcagt tctatttatt aaattataaa aagtttaata gtttatttta
     2401 ttgatatata gaaaatttag tagttcattt tattaaatta tagaaagatt aatggtttgc
     2461 tttattgaat tataaaaagt ttaataattt att
//
LOCUS       DAMJYR010000255         2416 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.260, whole genome shotgun sequence.
ACCESSION   DAMJYR010000255 DAMJYR010000000
VERSION     DAMJYR010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2416
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.260"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            25..498
                     /locus_tag="PC471_003818"
     CDS             25..498
                     /locus_tag="PC471_003818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDF2790894.1"
                     /translation="MFKEYLIRELKEDDIKNALDLIWNVFEEFESPDYCDEGITTFHQ
                     FIEYNSIIEKIKEKTLFFWGCFDSKKLIGVLAIRNLNHICMLFVDKNYHKHGVAKKLM
                     TTGIQYCICNKFNLTEITVNSSPYAVEVYHKLGFKDTAVEQIVDGIRFTPMKLLI"
     gene            complement(867..1424)
                     /locus_tag="PC471_003819"
     CDS             complement(867..1424)
                     /locus_tag="PC471_003819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDF2790895.1"
                     /translation="MRVSREIVIQATSDIVNKDGLNKVSLKVIAEKLGIRTPSLYNHI
                     ESLDDLLLEVAHKGMREMNSQMIKATIGNFGDTAIKCISIAYFNFIISNPGVYETIQW
                     ATWHGNNETLEIFDEYKSLLEKLILSCNLNTKNTGEVLNLIMSVLHGYSTLQLGKSLL
                     SKEEAIKGLADSIDTVLLGIHKKYD"
     gene            1682..2380
                     /locus_tag="PC471_003820"
     CDS             1682..2380
                     /locus_tag="PC471_003820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDF2790896.1"
                     /translation="MEKKYFEIHGVPSILWGNPSSKLYIYIHGQCGNKEEAEAFANIA
                     VPHECQVLSVDLPEHGERKREKNSFNPWHVVPELVSIMEYAKCHWNQISLYANSIGAW
                     FSMLSFEKENLRECIFVSPILDMQQLISNMMLWANVSEEQLKHELIIPTSFGHTLSWE
                     YLQYVKQHPITQWNVPTKILYGKHDELTEIGVVEKFVEKFNCNLTIIENGKHWFNTPQ
                     ELNSLYEWLNTLVK"
BASE COUNT          890 a          319 c          300 g          907 t
ORIGIN      
        1 ataataataa aggtgttgtt tataatgttc aaagaatact taataagaga attaaaagaa
       61 gatgatatta aaaatgcatt agacttaatc tggaacgtat ttgaagaatt tgaatctcca
      121 gattattgtg atgaaggaat tacgacattt catcaattta tagaatataa ctctattatt
      181 gaaaaaatca aagaaaaaac tctcttcttt tggggttgtt ttgactctaa aaaacttatt
      241 ggagtcttag ctattagaaa tttaaatcat atatgtatgt tatttgtaga taaaaactat
      301 cataaacatg gtgttgcaaa aaaattaatg actacaggca tacaatactg tatttgtaat
      361 aaatttaatt taactgaaat aactgtaaat tcatcacctt atgctgttga agtttatcat
      421 aaattaggat ttaaagatac tgctgttgag caaattgttg acggtatacg ttttacacct
      481 atgaaattgt taatataata aattaaataa aaagcaaagt ctcttaaata ttttatagtt
      541 actttgcttt taaaccaaga tttaaaataa tactatcttt tttgtttttt aataatatac
      601 tttaaaatcc ttactaatat gaactataat ttattttttg atatcctttt tgattaacat
      661 ataaattaat tcaattatta aacataaact atgttgtttc tctttatatc tcttctttgt
      721 aaacaatatt gggcatttga aaataaatat tcttaaaata tactgttctc ctgttgattt
      781 tcctttggta caaatgataa aaatttaatt tacatgcccc ataatgaagt gttcaataaa
      841 taagttaaaa taatttattt acctccttaa tcatattttt tatgaatacc taataacaca
      901 gtatcaatag aatcggctaa accttttata gcttcttctt tactcaaaag tgatttccca
      961 agttgcaaag tcgaataacc atgaagtaca ctcattatta agtttaatac ttcacctgta
     1021 ttctttgtat ttagattaca agaaagaata agtttttcta ataaggattt atattcatca
     1081 aagatttcta atgtttcatt atttccatgc caagtagccc actgaattgt ttcataaaca
     1141 ccaggattag aaattataaa attaaagtat gctatactta tacactttat agctgtatct
     1201 ccaaaatttc caatagtcgc ctttatcatt tgactattca tctctctcat tcctttatga
     1261 gctacttcaa gtaataaatc atctagactt tctatatgat tgtacaaaga tggtgtacgt
     1321 attcctagct tttctgcaat aacttttaaa gaaactttgt taagcccatc tttattaact
     1381 atatctgatg tagcttgtat aactatttct ctactaacac gcatgatttt ccctctttca
     1441 tttaaaatta attactttat tagtttagct aatctaatta gtttcgtcaa ttattgtaat
     1501 ttataaaatt ctttatacta ttataaaatc ttgacagttt aattataatt taatataata
     1561 ctaattgaat tagttttaat actaatataa ttagtattat attaaattct atattacaac
     1621 attattttat agtttgatta atttaacaat cactaatata tcaaactgaa aggagatttt
     1681 tatggaaaaa aaatatttcg aaattcatgg agttccttca atcctttggg gaaatccttc
     1741 aagtaaatta tatatttaca tacatggaca atgtggcaat aaagaggagg ctgaagcttt
     1801 tgctaatatt gcagttccac atgagtgtca agttttaagt gttgatttgc ctgaacatgg
     1861 agaacgtaaa agagaaaaaa attcatttaa cccatggcat gttgttccag aattagtatc
     1921 tattatggaa tatgcaaagt gccactggaa tcaaatttcg ttatatgcaa atagcattgg
     1981 agcatggttt agtatgttaa gctttgaaaa agagaactta agagagtgta tttttgtatc
     2041 tccaattcta gatatgcaac agctcatttc aaatatgatg ctttgggcaa atgtatcaga
     2101 agaacagctt aaacatgaac taattatccc aacttcattt gggcatactc tttcatggga
     2161 atatctacaa tatgtaaaac aacatcctat cactcaatgg aatgtaccaa caaaaatatt
     2221 atatggtaaa catgatgaac ttactgaaat cggtgttgta gagaaatttg tagaaaagtt
     2281 taattgcaac cttactatta tagaaaatgg caaacattgg tttaacacac cacaagaatt
     2341 aaattcatta tatgaatggt taaatacttt agttaaataa aaaataatta tattattaat
     2401 atgattaaat tattta
//
LOCUS       DAMJYR010000256         2389 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.261, whole genome shotgun sequence.
ACCESSION   DAMJYR010000256 DAMJYR010000000
VERSION     DAMJYR010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2389
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.261"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            324..2219
                     /locus_tag="PC471_003821"
     CDS             324..2219
                     /locus_tag="PC471_003821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp8"
                     /protein_id="HDF2790897.1"
                     /translation="MLSNKKRSMAIVMAGATVMSAAAPIFADNTVTENVDKNYTVSAK
                     DSAKLIEEVRKALEVKFEDTKAGANVNDRVYDIKVDNVNLTNATQLQNKINSLTEGQS
                     LKVTIQDKGHQVLGGKVVDYKIENYKTAQEIVDAVNAYNATLAEDSDNKLTATIKSTN
                     TVEVKRAKDSANVITLNVGDQHLDFSKVITSEEGTFEGYEKSYSDIDSKELHTITVKN
                     ADLQDISAEELFDGIRLTTLGREIVNKVKNGYALTFENEAILTQEQEDSDDKDKPEKS
                     SFDIVLSKANEKPETISVSSKNHKLVRDLHKVLTDVKGGKELKVEVLSGDSRFTTAVE
                     VSKERFKDGEAEAIILVGEDAIVDGLASAPLASQKNAPILLSKKDSLPSEIEAEILRV
                     LGSNLSSKKIYIVGGESKVSKETEEKLSKLGVSKVERVSGEDRFETSLEIAKQLKDTF
                     KTAFVVGGNGEADAMSISARAAQFGAPIIVTGNELDANAEKLLKGKELEIVGGENSVS
                     KEVEDKLVDIDLNNKVERLAGENRKDTNAKVINKYYAGATKAYVAKDGYVGGNGQLVD
                     ALTAAPLAASSKAPIVLTTEELSKSQEEVVELRLKNATKLVQIGEGIAKNAIEKIAEK
                     INLFTKN"
BASE COUNT         1042 a          242 c          442 g          663 t
ORIGIN      
        1 gatattaatt tagtgatata tatttaagta ataagagtaa agcaaaaatt agtgcatagt
       61 agtatataaa aatggagttt gaatactttt gttagtatat gattgtttag tagaaaatca
      121 ctataaaata ggatttctat ctttgagcat aattatagag agctaagcag ggggaagggg
      181 ttcaagaaga tattattagt gtttgtgtta gtaaattatg taaacacata aaatatattg
      241 aaaaataata ttatatataa tattatttat gtgtgggaaa tacgaatata aaaaataaaa
      301 aataatattc ggaggacata aatatgttaa gcaacaaaaa gagaagtatg gcaatagtaa
      361 tggcaggtgc tacagttatg agtgcagcag caccaatatt cgcagacaat actgtgacag
      421 aaaatgttga taaaaattac acagtaagtg cgaaagattc tgctaaacta atcgaagagg
      481 ttagaaaagc attagaagtt aagtttgaag atacaaaagc aggagcaaat gtaaatgata
      541 gagtttacga tataaaagta gacaatgtaa acttaactaa tgcaactcaa ttacaaaata
      601 agataaactc tttaacagaa gggcaaagtt taaaagttac tattcaagat aaaggacatc
      661 aagtgttagg tggaaaagta gttgactata aaattgaaaa ctacaaaact gctcaagaaa
      721 tagtagatgc agttaatgct tacaatgcaa ctttagcaga agattctgat aacaagttaa
      781 cagcaactat aaaatctaca aatacagttg aagtaaaaag agcaaaagat tcagcaaatg
      841 ttattacatt aaatgtagga gaccaacatt tagatttctc aaaagtgata acatcagaag
      901 aaggtacttt tgaaggttat gaaaagagtt atagtgacat agattcaaaa gaattacata
      961 cgataacagt taaaaacgca gatttacaag acatatcagc agaagaatta tttgatggaa
     1021 ttagattaac tacattagga agagaaatag ttaataaagt taaaaatgga tatgctttaa
     1081 catttgaaaa tgaagcaatc cttactcaag aacaagaaga tagtgatgat aaagataaac
     1141 cagaaaaatc aagctttgat atagttttaa gcaaggcaaa tgaaaagcca gaaacaataa
     1201 gtgtttcaag taaaaaccat aagttagtta gagacttaca caaagtatta actgatgtaa
     1261 aaggtggaaa agaattaaaa gtagaagttt tatctggtga ttcaagattt acaacagcag
     1321 tagaagttag taaagagaga ttcaaagatg gagaagcaga agctataatt ttagttggag
     1381 aagatgctat agttgatgga ttagcatcag caccacttgc ctctcaaaaa aatgcaccaa
     1441 tattattatc taaaaaagat tcactaccat cagaaataga agctgaaata ttaagagtac
     1501 ttggaagtaa tctatcttct aagaaaatat atatagtagg tggagaatct aaagtatcaa
     1561 aagaaactga agaaaaactt tctaaattag gtgtaagtaa agttgagaga gtttctggag
     1621 aagatagatt tgaaacttct ttagaaatag caaaacaatt aaaagataca tttaagactg
     1681 cttttgtagt aggtggaaat ggagaagctg atgctatgag tatatcagct agagctgctc
     1741 aatttggtgc tccaataata gttacaggta acgaattaga tgcaaatgct gaaaaattat
     1801 taaaaggaaa agaattagaa atagtgggtg gagaaaattc tgtatcaaaa gaagttgaag
     1861 acaaattagt tgatatagat ttaaataata aagttgaaag attagctgga gaaaatagaa
     1921 aagatactaa tgctaaagta atcaataaat actatgcagg tgcaactaaa gcatatgtag
     1981 caaaagatgg ttatgtaggt ggaaatggac aattagttga tgcacttaca gcagcaccac
     2041 ttgcagctag ttcaaaggct ccaatagtat taactacaga agaactttct aaatcacaag
     2101 aagaagtagt tgagttaaga cttaagaatg ctactaagtt agtacaaata ggtgaaggaa
     2161 tagctaaaaa cgctattgaa aaaatagcag aaaaaataaa cttatttact aagaactaat
     2221 aagatatagg aattaatatt aaaagtttgt ttagataata tatgattata caatgcgtta
     2281 atatgtaaga catagtgtag atattataaa atatacaaat aacaagataa aataataaaa
     2341 ataaggtgtc tcatttagaa ttagattgag ataccttatt ttatttttt
//
LOCUS       DAMJYR010000257         2383 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.262, whole genome shotgun sequence.
ACCESSION   DAMJYR010000257 DAMJYR010000000
VERSION     DAMJYR010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2383)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2383)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2383
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.262"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            110..556
                     /locus_tag="PC471_003822"
     CDS             110..556
                     /locus_tag="PC471_003822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HDF2790898.1"
                     /translation="MDFKNLQYESLSYIICNLQKNFKLYCEKCLKPYKLTNGLYFYLI
                     YINKNRNCSLNDVSTEFEVDKAHTTRTISRLEQDGYIEKIQNPNDSRAFQLRVTDKGE
                     EVLGDIKNIFSKWDNHIKKEFSDTEYKELVKNLHVVKDIKTAVEEE"
     gene            558..1652
                     /locus_tag="PC471_003823"
     CDS             558..1652
                     /locus_tag="PC471_003823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase"
                     /protein_id="HDF2790899.1"
                     /translation="MYCNLLKPIKIGNLELKNRVSFAPTSMGLKLEEKIKKFSDIAKS
                     GVALITLGDVSIRPSFHKVAISLSDEDGVMKYKKIVDEIHNSGAKVSAQLFCSDYDVN
                     LIKDTMNMGITSHDEIKKIMNDGVKDYITNMPKEEIKNIINLFKVTALNAKKAGFDMI
                     QIHGDRLVGSFSSSIFNNRNDEYGRTCDNRSRFASEIISSIRDEVKDIPIDYKFAIRQ
                     ENPHYGNAGVLLSEVEYFVKKFESLGVNSFHVTLANHSKLEDTIPTNNHPYFKEEGCF
                     LYLADEVKKHTNLPVCGVGKLSSPDFIESIISNNRVDMVSMSRQLLADSNWLQKVKDG
                     RVDEIKKCCYCNKKCADALQTRSQFGCILD"
     gene            1740..2270
                     /locus_tag="PC471_003824"
     CDS             1740..2270
                     /locus_tag="PC471_003824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HDF2790900.1"
                     /translation="MKSLVLYSSLTGNTKKIAYTIYDEIQEEKDIKDVNELVDYGIDY
                     ENYDIVFLGYWVDKGICDKNSKQVLENIHNKKIALFGTMGASKKGSYGASIIEKIESI
                     IPKDNEILGSFICQGKIAEGLKAKYKEMLKLSPDNEHIRQQLNNHEESQSHPDEQEIY
                     EASMFAKNMMIKASIV"
BASE COUNT          996 a          232 c          418 g          737 t
ORIGIN      
        1 ttaataaaac atagatttag agaataaaat attcttgact atattaattg tttaatataa
       61 tatagttgat atagtcaaac atattataat tatagaaggg aaatttaaga tggattttaa
      121 aaatttacag tatgaaagtt tgtcatatat tatttgtaac ttgcaaaaaa actttaaact
      181 ttattgtgaa aaatgtttaa aaccatataa acttacgaat ggtctatatt tctatttaat
      241 ttatattaat aaaaatagga attgttcttt gaatgatgtt tctacagaat ttgaagttga
      301 taaagctcat acaacaagaa ctatatctag gttagaacaa gatggataca tagaaaaaat
      361 ccaaaaccct aatgatagta gagcttttca gttgagagta acagataagg gtgaggaagt
      421 attaggggat ataaaaaaca tattttcaaa gtgggataat cacataaaaa aagagttctc
      481 ggatacagaa tataaagaac tggtaaagaa tttacatgtg gtaaaggata taaaaacagc
      541 tgtggaggaa gaataggatg tattgtaatt tattaaaacc aattaaaatt ggaaatttgg
      601 aattaaaaaa tagagtatct tttgcaccta caagcatggg tcttaaattg gaagaaaaaa
      661 ttaaaaagtt ttctgatatt gctaagtcag gagtagctct gattactcta ggagatgtat
      721 ctattagacc tagttttcat aaggtagcta taagcctatc ggatgaagat ggagtcatga
      781 aatacaagaa aattgtagat gaaatacaca attctggtgc aaaggtatca gcacaactat
      841 tttgttcaga ttatgatgtt aatttaatta aagatactat gaatatggga attacttcac
      901 atgatgagat aaaaaaaata atgaatgacg gagtaaaaga ttacataaca aatatgccca
      961 aagaagaaat taaaaatatt ataaatttat ttaaagtaac agcattgaat gcaaaaaaag
     1021 caggttttga catgattcaa attcatggtg ataggcttgt aggaagtttt tcatcaagta
     1081 tatttaataa tagaaatgat gaatatggta gaacgtgtga taatagaagt agatttgcaa
     1141 gcgaaataat tagtagtatc cgagatgaag ttaaggacat tccaatagac tataaatttg
     1201 caataagaca agaaaatcct cattatggaa atgcaggagt tttactttcg gaagttgagt
     1261 attttgttaa aaaatttgaa tccctaggtg taaacagttt tcatgtaaca cttgcaaatc
     1321 attcaaaatt agaagatact ataccaacca ataatcatcc ttactttaaa gaagaaggct
     1381 gttttttata tttagctgat gaagtaaaga aacacacaaa tcttccagta tgtggggttg
     1441 gaaaattgtc tagcccagat tttatagaaa gtattatttc aaataataga gtagatatgg
     1501 tttctatgtc aagacaatta ctagcagata gtaattggtt acagaaagta aaagatggaa
     1561 gagtagatga gattaaaaag tgctgttatt gtaataagaa gtgtgcagat gctttacaaa
     1621 cacgctctca gtttggatgt atactagatt agaatatcct tataaaaata aataatttgt
     1681 taaggttaat atatttttgg tgatttagat aaatatttag ataaagagga ggagacatca
     1741 tgaaaagttt agttttatat tcaagtttaa ctggaaatac taaaaagatt gcttatacta
     1801 tatatgatga aattcaagaa gaaaaagaca ttaaagatgt aaatgaatta gttgattatg
     1861 gtattgacta tgaaaattat gatatagtat tcttaggcta ttgggtggac aagggaattt
     1921 gtgataaaaa ttcaaaacaa gtgcttgaaa atattcacaa taaaaagata gcactatttg
     1981 gaacaatggg agcaagtaaa aaaggaagtt atggagctag tataatagaa aagatagaat
     2041 caattatccc aaaagataat gaaatactag gttcattcat atgtcaaggt aagatagcag
     2101 aaggattgaa agcaaaatat aaagagatgt taaaactttc tcctgacaac gaacatattc
     2161 gccaacaatt aaataaccat gaggaatctc aaagtcatcc agatgaacaa gaaatatatg
     2221 aagccagtat gtttgcaaaa aatatgatga ttaaagctag tattgtgtag tagttaaaca
     2281 ataagttcat ataagatata gaataaataa tttagtaaaa aagtatagca aataatttgc
     2341 tatgcttttt ttatgaaatc aaaaaaataa taaaaatact tga
//
LOCUS       DAMJYR010000258         2382 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.263, whole genome shotgun sequence.
ACCESSION   DAMJYR010000258 DAMJYR010000000
VERSION     DAMJYR010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2382)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2382)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2382
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.263"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            1108..2379
                     /locus_tag="PC471_003825"
     CDS             1108..2379
                     /locus_tag="PC471_003825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HDF2790901.1"
                     /translation="MLKVLKGNIIFTKTSETFTVFENSYIMLEKGKVKGIFKEIPKEY
                     KNLKVVDYTDKLIIPGMNDLHAHASQFKNLGMAMDKELLPWLETYTFPEESNYKDIEY
                     ATKMYKKFVKELWKSGTTRIAVFATVHKEASMKLMDIFKEVGLGAYVGKVNMNMNCPD
                     TLLENTQDSITDTEEIINKYSDTDSIVKPIITPRFIPSCTSELLKGLGDLCLKYNIPI
                     QSHLSENYDEIEWVRSLEPESKFYGDAYNRYNLFGQTPTLMAHCVHCSQDEIDLMREN
                     NVMAVHCPASNFNVGSGAMPIRKLIDNNIRLALGSDISGGHTLSIFKAMVSAIQLSKL
                     YWVNSGKKYNFLSLSEAFYIATKSGGSFFGKVGSFEEGYDFDALIIDDSNLNHDNYSI
                     LERLERFIYVGDDRNIIHRYVCGKLIEEPNI"
BASE COUNT          936 a          299 c          321 g          826 t
ORIGIN      
        1 tatctggtaa ttatttacta caaatgataa aaaataatta tattttaaag gataaaatgc
       61 tgtataaatt attggggtat tttacctata cttgttacaa atttacatac cacttagtta
      121 atataaaaca aatttcaaag agattggaca acgaataaaa attgcaattt atataccata
      181 tagttaatat aaagcatatt tgtgtcgata tatagttcta actaagtata agatttacat
      241 acctcatagt taatataaaa cacatatttt acatgtttga agattttatc agaaacccta
      301 tttacatacc gcatagttaa tataaaacta ttaatagtgc tatgttgttt atttgcatta
      361 acaagattta cataccacat agttaatata aaacatgttt agcaggagtt atgatgataa
      421 aacacattat ttatttacat accacttagt taatataaaa ccccaaaata aactaagtat
      481 tttcagtact tacacacata caactctctc aaatttgcag tgaaccatga gtagtgcaat
      541 tgataacatt tatcatacac cctcaatccc ttacattgca actgttaaac cctactttat
      601 caaaaatatc gctcactgca aaatttatat atttttatta tatcataaaa ttcaaactat
      661 aaaattaaaa tggaataatt tatcaattta agaacaccta catatttgta aatacttact
      721 atattatcta ttctaatagt tcttcttcta gctagaacta atataaaaat catctctgaa
      781 agattaggac atactaatat aaaaataact atgaatagat attttcttgt tttaaacgat
      841 atggataaaa aaacttctga aaatataatc aaagttctct ttaaataaca ttccatttta
      901 tgtaagtagg cattatattt ttcttaaatg tcacttaaat gtcagttata tataataata
      961 gtaaacaact gtatttcact aatatttgct attatctaat agtatttata gtttagattt
     1021 gatataatac atgtggtaaa attttgattt gtaaaacaaa attttaccta catatattaa
     1081 aacaaattta aaactaagga gacctatatg ttaaaagtac taaaaggaaa tataattttt
     1141 acaaaaactt cagaaacttt cacagtattt gaaaatagtt atattatgct ggaaaaaggt
     1201 aaagtaaagg gaatatttaa agagatacct aaggaataca aaaacctaaa agtagtagat
     1261 tacactgata agcttataat acctggtatg aatgacctcc atgcccatgc ctctcaattt
     1321 aaaaatcttg gaatggctat ggataaagag cttttgccat ggcttgaaac ttatacattt
     1381 ccagaagaat caaattataa agatatagaa tatgcaacaa aaatgtataa aaaatttgta
     1441 aaagaacttt ggaaatctgg aactacaaga atagcagtgt ttgcaacagt acataaagaa
     1501 gcctctatga aacttatgga tatattcaaa gaagttggtc ttggagcata cgttggtaaa
     1561 gtaaatatga atatgaattg ccctgatact ttattagaaa acactcagga ttctattact
     1621 gatacagaag aaattataaa taagtatagt gatacagatt ctattgtaaa accaataata
     1681 acacctagat ttataccaag ctgtacaagt gaacttttaa aaggtttagg agatttatgt
     1741 ttaaaatata atattcctat tcaatcacac ttatctgaaa attatgatga gattgaatgg
     1801 gttagaagtc tagaacctga atctaaattc tatggagatg catataatag atataattta
     1861 tttggtcaaa ctcctacttt aatggctcat tgtgttcatt gttctcagga tgaaatagat
     1921 ttaatgagag aaaataatgt tatggcagtt cactgtccag cttctaactt taatgttggt
     1981 agtggtgcta tgcctatcag aaaattaatc gataataata ttagattagc acttggatca
     2041 gatataagtg gtggacatac attgtcaata tttaaagcaa tggtatctgc aatacaatta
     2101 tctaaattat attgggttaa ctctggtaag aagtataatt tcttatcttt atctgaggct
     2161 ttttatattg caaccaaaag tggtggaagt ttctttggaa aagttggtag ctttgaagaa
     2221 ggttatgatt ttgatgcatt gattattgat gattcaaatt taaatcatga caattattct
     2281 atacttgaaa gacttgaaag atttatatat gttggtgatg atagaaatat cattcataga
     2341 tatgtatgtg gaaaactaat tgaagaacct aatatataat ta
//
LOCUS       DAMJYR010000259         2344 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.264, whole genome shotgun sequence.
ACCESSION   DAMJYR010000259 DAMJYR010000000
VERSION     DAMJYR010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2344
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.264"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            134..1585
                     /locus_tag="PC471_003826"
     CDS             134..1585
                     /locus_tag="PC471_003826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine:cation symporter family protein"
                     /protein_id="HDF2790902.1"
                     /translation="MDFFNGILTSINNAVVYVNNYLWSYILIIMLVVIGIYFTIKTNF
                     VQFRYFIEMFRLLGDGTANKNAKKEGKISSFQAFCISTSSRVGTGNIAGIAIAVVAGG
                     PGAVFWMWLIALIGSASSFVESTLAQIYKVKDGQAFRGGPAYYMEQGLNKKWMGILFS
                     ILITICYGFVFNAVQANTVSLAFNNAFGISKMAMGIGLGILTAIVIFGGVHRVAKVSE
                     IIVPVFAGLYILVALAIVVMNITEIPSVIALIVESAFDFKGMAIGTFMGVVMTGVKRG
                     LFSNEAGMGSAPNAAATAHVTHPVKQGLIQSLGVFTDTIIICSCTAFMVLLYSGYSSS
                     GATGIELAQEALTHHIGPVGNIFIAVCIFLFAFSSIVGNYYYGESNMEFMSGSKTKLN
                     VFRVFVVGMVLFGSLTQVDVVWNLADLFMALMAIINLIAIALLGKYAFIALKDYTSQK
                     KSGIKDPVFIADEIEGLEKVSEWHRELSEEHLG"
     gene            complement(1804..2265)
                     /locus_tag="PC471_003827"
     CDS             complement(1804..2265)
                     /locus_tag="PC471_003827"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HDF2790903.1"
                     /translation="MIYEGKLIGKDLKIGIINSRFNEFITSKLLSGAEDCLLRHDVSP
                     ENIEIVWVPGAFEIPLVAQKMAKSGKYDAIICLGCVIRGATSHYDYVCSEVSKGIAKV
                     SLDSELPVIFGIVTTENIEQAIERAGTKAGNKGYDCAMNALEMANLFKSLN"
BASE COUNT          797 a          271 c          400 g          876 t
ORIGIN      
        1 tcattttaaa ccttgtaatt tgattttata aatattataa ttaaattaat gaatttttaa
       61 caaggaatta ttcatttttt tgattttaag atttattgag aaaacaaaac taaatagggg
      121 ggtaattaat ataatggatt ttttcaatgg tattttaacg agtattaaca atgcagtagt
      181 atatgtaaac aattacttat ggtcgtatat cttaattata atgttagttg taattggtat
      241 ttactttact attaaaacaa attttgtaca attcagatat tttatagaaa tgtttagact
      301 tctgggtgat ggtacagcga ataaaaatgc aaaaaaggaa ggaaaaatct cttcatttca
      361 agcattttgt ataagtacat cttcaagagt tggaactgga aatatagctg gaatagcaat
      421 tgcagtggta gcaggaggac ctggtgctgt attttggatg tggcttatag cattaattgg
      481 ttctgcatca agctttgttg agagtacatt agctcagatt tacaaagtga aagatggtca
      541 agcttttaga ggtggaccag cgtattatat ggaacaaggt ctaaataaaa aatggatggg
      601 tattttattc tcaatactta taactatatg ttatggtttt gtatttaatg cagttcaagc
      661 aaatactgtt tcacttgcat ttaataatgc atttggaata agtaaaatgg ctatgggaat
      721 aggtcttgga attttaactg caatagttat ttttggtgga gtgcatagag ttgcaaaagt
      781 ttctgaaata atagttcctg tatttgcagg attatatata ttagttgctt tggctatagt
      841 tgtaatgaat ataacagaga taccaagtgt tatagcattg atagttgaaa gtgcatttga
      901 ttttaaagga atggcgattg gtacatttat gggagttgta atgactggag tgaagagagg
      961 tctattttct aatgaagcag gtatgggtag tgctcctaat gctgctgcaa cagcacatgt
     1021 aactcatcct gtaaaacaag gtttaattca atctttaggt gtttttacag atactataat
     1081 aatatgtagt tgtacagcat tcatggtatt attatattca ggttatagta gtagtggagc
     1141 aactggaatt gaattagctc aagaagcttt aactcatcat ataggaccag ttggaaatat
     1201 atttatagca gtttgtatat tcttgtttgc atttagttct atagttggta attactatta
     1261 tggtgaatca aatatggagt ttatgagtgg aagtaagact aaacttaatg tatttagagt
     1321 ttttgttgta ggtatggttt tatttggttc acttactcaa gtggatgtag tttggaatct
     1381 tgcagactta tttatggcat tgatggcgat aataaactta atagcaatag cacttttagg
     1441 aaaatatgca tttattgctt taaaagatta tacaagtcaa aaaaaatcag gaataaaaga
     1501 ccctgtgttt attgcagatg aaatagaagg cctagaaaag gtatcagagt ggcataggga
     1561 attatctgag gaacatttag gataaaaaat agtataaaat tataaaacca ttttgaaaga
     1621 ctagagtatg tatatacttt atatgaatat aatattgaat taatttatat tactggaatt
     1681 tttaattcca gtaatatttt tttattatat aatttagctt tattaaaagt ttttattatt
     1741 aaaaatagct tatttatcgt aaagtgaatg atattgggtt ttaattatta tgaatgttag
     1801 tatttaattt aaagacttaa ataaatttgc catttctagc gcattcatag cacaatcata
     1861 acctttattt ccagcttttg tacctgctct ttcaatagct tgttcaatat tttctgtagt
     1921 tacgattcca aatataacag gtaattcact gtctaaagat actttggcta tccctttaga
     1981 gacttcacta catacataat cataatgaga agtagcccct cttataacac agcctaaaca
     2041 gattatagca tcatacttac cacttttagc cattttttga gcaacaagag gaatctcaaa
     2101 agctccaggt acccaaacta tttctatatt ttctggagaa acgtcatgtc ttaaaagaca
     2161 gtcttcagct cctgataaaa gttttgatgt tataaattca ttaaaacgag aatttattat
     2221 acctattttt aaatcttttc caataagttt tccttcatat atcattttta cttcctccaa
     2281 ttttatttat gttatagatt tatatttata aatatcttta atttaattat aaaaaactac
     2341 ttag
//
LOCUS       DAMJYR010000260         2246 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.265, whole genome shotgun sequence.
ACCESSION   DAMJYR010000260 DAMJYR010000000
VERSION     DAMJYR010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2246
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.265"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(140..1432)
                     /gene="brnQ"
                     /locus_tag="PC471_003828"
     CDS             complement(140..1432)
                     /gene="brnQ"
                     /locus_tag="PC471_003828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HDF2790904.1"
                     /translation="MGEKQINFKDVIVIGFALFAMFFGAGNLIFPPYLGVLSGSSWLV
                     AFIGFLFADGGLALLAVIAATKFNGDTSKMFSRAGKGLSIALGCAMVICIGPLLAIPR
                     TAATTYEMGILPTIGSGISPVIFSIVFFALVLVLTIRPSKVVDIVGSILTPALLIALA
                     VLIVKGIVSPLGEIRDSSLIQNVFAEGITQGYQTMDALAASVFASIIIMSVIAKGYTG
                     EKEKMKATISAGVIAVIGMALVYGGLCYLGATVSQQYGQDVQQTALIVAITAALLGTT
                     GKILLAIIVALACLTTAIGLSSAAGQYFSTLTDGKLKYEHIVIVVCVFSAIISNFGVS
                     TIIQFSSPILSMVYPATITLVILALFSNKIKNDNVFKCAAYMALLVSVLTVATSFGVK
                     IPLVNSLPFASLGFNWIVPVVIAGIIGNFIPSKSSKTA"
BASE COUNT          755 a          374 c          246 g          871 t
ORIGIN      
        1 aaaaataaat ttttaaattt atcaaacagg attaataatg ttcttttatc tacaataatt
       61 taataattct tttaaatata aaaaggaatt gcctatataa tttaaaggca attccataat
      121 tatcaattag tttttattat tatgcagttt tactgctttt agatggaata aagtttccta
      181 ttattcctgc tattacaact ggaactatcc agttaaatcc taatgaagca aatggtaagc
      241 tattaactag aggaattttt acaccaaaac ttgttgcaac tgttaataca cttactaata
      301 atgccatgta tgcagcacat ttgaacacgt tgtcattttt aattttatta ctaaataatg
      361 ctagtataac tagagttata gttgctggat ataccatact tagtatagga gaagagaatt
      421 gtattattgt acttactcca aagtttgata tgattgcact aaatacacaa actactataa
      481 ctatatgctc atactttaat tttccatctg taagtgttga gaaatattga ccagctgctg
      541 aagaaagacc tatagctgtt gttaaacatg ctaatgcaac tataatagcc aataaaattt
      601 tacctgtagt tccaagtaaa gctgctgtta ttgcaactat taaagctgtt tgttgaacat
      661 cttgtccata ttgttgagac acagttgctc caagataaca taatccacca taaactaatg
      721 ccattcctat tactgcaatt acacctgcac ttattgtggc tttcatcttt tctttctcac
      781 ctgtataacc tttagctatt actgacatga ttataattga tgcaaataca ctagctgcca
      841 atgcatccat tgtttgataa ccttgagtta taccttctgc aaatacattt tgtattaaag
      901 aactgtctct tatttctcct aatggagata cgataccttt aactattaat actgctaaag
      961 ctataagtaa agctggagtt aatattgacc caactatatc tacaactttt gatggtctta
     1021 ttgttaaaac caatactaga gcaaagaata ctatagagaa tattactggg cttattccag
     1081 aacctattgt aggtaatata cccatttcat aagttgttgc tgctgttcta ggtattgcta
     1141 aaagtggccc tatacagatt accattgcac atcctaatgc aatacttaat cctttgcctg
     1201 cccttgagaa catcttcgat gtgtctccat tgaacttagt tgctgctata actgctaata
     1261 atgccaaacc accatctgca aaaaggaatc ctatgaaagc tactagccaa ctacttccag
     1321 ataaaactcc tagatatgga gggaagatta agttccctgc accaaaaaac attgcaaata
     1381 gtgcaaatcc aattacaatt acatctttaa aattgatttg tttctcaccc atttaaagca
     1441 cccccatttg aattttacta ccttttacta aaaaataagt ccaatctctt aattaaatga
     1501 aaaccttttg acgagatttt atttaattat acatttttga cttaaatttg tcaattatgt
     1561 ttcataattt ataaaatctt cacaatattt aatacaactt tcttaattaa acaaaatcaa
     1621 cctgtatact ttaaacatag ttggtttttt gttttttctc taatttttct tgataaaatt
     1681 aactattccg aataattaaa atattaagac tttttagatt attcgattta ttattaagtt
     1741 gtattgcttt atatactgta tatatcactc tcctttttta cattcatata atctttttaa
     1801 ttcaatttaa attttaaata ttttgtgttt attatataat ctgaaaaaaa agcagtcaat
     1861 agcattttta atttttttaa ttttcaaaat tatgattctt aaaaaatcgt tttcctttta
     1921 tcataacacc tatttacgtt aattcttgta gtattttgta tttattttgc taaattgaca
     1981 gttaacttta ttcaattttt attctacttg cgtagatttg cctcttgttt tttactctta
     2041 aaaccatttt ttctgaaaat tccttattat gatttatcaa cttatttttc ttcataacaa
     2101 ttactctcta acttccaact tacttaaact ttgcagttgc tagtattcac gtttacatac
     2161 ttatatgtca taattaacta aaaattctta atatatctct caatgaaaac aatttactca
     2221 tttatttaat acatttatat aatcat
//
LOCUS       DAMJYR010000261         2044 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.266, whole genome shotgun sequence.
ACCESSION   DAMJYR010000261 DAMJYR010000000
VERSION     DAMJYR010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2044)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2044)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2044
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.266"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(44..1261)
                     /locus_tag="PC471_003829"
     CDS             complement(44..1261)
                     /locus_tag="PC471_003829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallo-hydrolase"
                     /protein_id="HDF2790905.1"
                     /translation="MLGKKCMDYLQTLGKISSTTNGLTRLILTQEHKKSIDLISSWME
                     GLNLDIEIDDIGNVIGTYKSSFPNAPTLVVASHQDSVKCGGIFDGMLGIIVPLVGLEE
                     AKHNNRSYPFNIKLIAFAEEEGTRFETSLMGSKVFAGTFKEELLKSVDENGITLEEAV
                     TKFGFNTKNLTNLHPRKDVDAYLEFHIEQGPVLENESLPAGIVSSITGFKSFKISVNG
                     KSGHAGTLPMNMRLDAGCCACECVLAIEKVAKTTTDLVATVGKMNFYPSSSNVVPERA
                     EFTLDVRSCSQEILDNSVEKIFNEISHICENRKLNYTSELAFENVPVPCSNKITKIIE
                     KSFIDLNLNPFYIYSGAGHDAQEMDNITDIGMVFIRCAGGVSHNPNESVSVDDLDTAV
                     KIFLKILDNLDLK"
     gene            complement(1452..1865)
                     /locus_tag="PC471_003830"
     CDS             complement(1452..1865)
                     /locus_tag="PC471_003830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDF2790906.1"
                     /translation="MELEITEKIEKQDEDTIFQGLLKYNLARLEDKNPVDLGIYIRDK
                     AGKIVAGFIGVTHGNWLSIKYLWVSEKLRYKGTGSQLLYKAEKIAKERGCKYVFLDTF
                     SFQAPKFYEKFGYHKVFSLENYPLTGKRYYYRKIL"
BASE COUNT          699 a          327 c          230 g          788 t
ORIGIN      
        1 attttttaga ttatatattt taatatactt tcttatataa gaattatttt aaatcaagat
       61 tatctagtat ttttaaaaat atttttacag cagtatccaa gtcatctaca gagacacttt
      121 cattaggatt atgacttacg cccccagcgc atcttataaa taccatacca atatcagtta
      181 tattatccat ttcctgagca tcatggccag caccactata aatataaaaa ggattaagat
      241 ttaaatctat aaaacttttt tctatgattt ttgttatttt attagaacat ggtacaggaa
      301 cattttcaaa agcaagttca cttgtataat taagttttct attttcacat atatgtgata
      361 tttcattaaa tatcttttct acagaattat caagaatttc ttgtgaacaa gagcgtacat
      421 cgagtgtaaa ttcagctctt tcaggtacca catttgatga agatggatag aaattcatct
      481 ttcctacagt agcaaccaaa tcagtagtcg ttttagctac tttttcgata gctaatacac
      541 attcacaggc acaacatcca gcatcaagtc tcatattcat tggaagtgta ccagcatgtc
      601 cagacttacc atttactgat atcttaaaac ttttaaatcc tgtaatactt gatacaattc
      661 cagcaggaag agattcattt tctaaaacag gaccctgctc aatatgaaat tctaaatatg
      721 catctacatc ttttctagga tgcaaatttg ttaagttttt tgtattaaat ccaaactttg
      781 ttacagcttc ttcaagtgta attccatttt catcaacaga tttaagaagt tcctctttaa
      841 aagtaccagc aaaaacttta cttcccatta aagaagtttc aaatctagtt ccttcctctt
      901 cagcgaaagc aattagctta atattgaaag gataagacct attattgtgt tttgcttctt
      961 caagaccaac tagaggcact attataccta acataccatc aaatatacct ccacacttta
     1021 cactatcttg atgagatgca actactaatg tgggagcatt tggaaagctt gatttatatg
     1081 tacctataac gtttccaatg tcatcaattt caatatcaag atttaaacct tccatccatg
     1141 aactaattaa atcaatagat tttttatgtt cttgagtcaa gataagtctt gtaagaccat
     1201 ttgttgttga acttattttt cctaatgttt gtagataatc catacatttt tttcctaaca
     1261 tatttacctc ctaaaaatat aatttttatt taagctaaga ttagctttta tacttataat
     1321 ttttgaactt tcaaataatt ttgtatgtat agtaaaatac tattttagta ttaataatga
     1381 tataaaaaca taaagtctgt agacgaagag cagaagtttt aatttaaaaa tatgaaatat
     1441 gatttttatt attatagtat ttttctataa taatatctct ttccagttag aggataattt
     1501 tcaagtgaaa atactttatg gtatccaaat ttctcataaa atttaggtgc ttgaaaactg
     1561 aatgtatcta aaaaaacata tttacaacct cgttctttag caattttttc agccttatat
     1621 aaaagttgac ttccagtacc tttatatcta agcttctcac taacccataa gtattttata
     1681 gaaagccaat taccatgagt aacacctata aaacctgcaa caattttacc tgctttatct
     1741 cgaatatata ttcctaaatc cactgggttt ttatcttcaa gtctagcaag attatatttt
     1801 aataatcctt gaaaaatagt atcttcatct tgtttttcta ttttttctgt aatttctaat
     1861 tccatatttg aactttctcc ttgtataatc ttttgtatat tggtattttt taataaaatc
     1921 agtagaaaga atatagttta acattattct aataggcatt tatgctaatg tcaaactaat
     1981 aatacttatt cttaatttta tattgttgat attaaatttt tttaaataaa aatgtagttt
     2041 aagt
//
LOCUS       DAMJYR010000262         2032 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.267, whole genome shotgun sequence.
ACCESSION   DAMJYR010000262 DAMJYR010000000
VERSION     DAMJYR010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2032)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2032)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2032
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.267"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            389..682
                     /locus_tag="PC471_003831"
     CDS             389..682
                     /locus_tag="PC471_003831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HDF2790907.1"
                     /translation="MGFPETFKALSDPIRREILVMLRENKMSAGEISEKFNMTNATIS
                     YHLSQLKKAGLIFETKYKNFIFYEINISVFEEVMLWFSQFMEKGEKDNEKSDK"
     gene            663..1304
                     /locus_tag="PC471_003832"
     CDS             663..1304
                     /locus_tag="PC471_003832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HDF2790908.1"
                     /translation="MKKAINKQFILSTLICFIPFIVSIYFYNRLPNEVAIHFDNYGNP
                     DNYAPKVIAAFGVPLLMLCIHLYTWFRLENEPKKANFSNVIQNLSKWGVAILSVIIQI
                     MMVLYSTGVSLESNFYTGLILGIIVILFGNYLPKCKRNYSVGIILPWTLNDEDNWNKT
                     HHLAGWIWLIGGILLIINAFINIPFYNIFVIFVIVILPFIYSYLMYKISGKNN"
     gene            1548..1880
                     /locus_tag="PC471_003833"
     CDS             1548..1880
                     /locus_tag="PC471_003833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HDF2790909.1"
                     /translation="MNTREMKEYRGFYNKKLSETDEFFRVFGSLDDKTYSEGAISKKN
                     KELMGLAISILSRCDECICYHIEGCINSGASKDEIIEAIKIGVIGGGSITYPNARFAI
                     KILEEFNI"
BASE COUNT          778 a          185 c          311 g          758 t
ORIGIN      
        1 gtatctaatt agtagatgtt tttatgtact tttatacagt atatttctcg tttcattctt
       61 ttctatttaa ataggactta actacttttt aaattgggtt tagctacttt taatttaaaa
      121 ttaaaagtat tgttatatat ataagtacct caatcattag gtttttgctt taacaattat
      181 ggtgcaatac acttttgtta agttaaaaaa attagataaa gtaagattaa ataaaagcga
      241 cttaagtaaa ttgagaatct taatataaaa attttcaata aatatttaga aataactatt
      301 tagaaaaatt tctaaatagc tattgaaata aaatggatgt tatgttatat tctatttaga
      361 tgaaattcta aatagcgagg tgatggtgtt ggggtttcca gaaacattca aagctctttc
      421 agaccctata agaagagaaa tattggttat gttgagggag aataaaatgt cagcaggaga
      481 aattagtgaa aaatttaata tgacgaatgc tacaatttct tatcatctat ctcaactaaa
      541 aaaggcagga ctcatatttg aaacaaagta taagaacttt attttctacg aaataaatat
      601 ttcagttttc gaagaggtca tgctttggtt ttcacaattt atggagaagg gagaaaaaga
      661 taatgaaaaa agcgataaat aaacaattta ttttatctac tttaatatgt tttataccat
      721 ttattgtatc aatatatttt tataatagat taccaaatga agttgcaatt cattttgata
      781 attatggaaa tccagataat tatgctccaa aggttatagc agcttttggt gtaccattgt
      841 taatgctatg tatccatttg tatacatggt ttagacttga gaatgagcct aagaaagcta
      901 atttttcaaa tgttatacaa aatttatcaa aatggggagt tgctatactt tcagtaatta
      961 tacaaataat gatggtttta tattcaacag gagttagttt ggagtctaat ttttatacag
     1021 gattaatttt aggaataata gttattttat ttggaaacta tcttcctaaa tgtaaacgaa
     1081 attactcagt aggaataatt ttaccatgga ctcttaatga tgaagataat tggaataaga
     1141 cacaccattt agctggatgg atttggctta ttggtggaat attattaatt attaatgcat
     1201 ttattaatat tcctttttat aatatctttg taatctttgt aattgtaata ctaccattta
     1261 tatattctta tttgatgtat aaaatttcag gtaaaaataa ctaatataat taatcactat
     1321 aataatgttt ggataagaat aatatatttc agaactgaga tagataataa agaaattact
     1381 aagatttcta ttttagttct gaaaaaatct tacttatatg ataacttatt gagatttcaa
     1441 aaaataatat aaattaggct aatgaacata aaatgaaaat ttgtatttaa tattgaaaat
     1501 ataagtacgg tgtgatattc ttataagaaa aacataggag gtagaagatg aatactagag
     1561 aaatgaaaga atatagggga ttttacaata aaaaattaag cgaaacagat gagtttttta
     1621 gagtatttgg aagtcttgat gataagactt attcagaagg ggctataagc aaaaaaaata
     1681 aggaattaat gggactagct atttcaattt taagccgatg tgatgagtgt atttgttatc
     1741 atattgaggg ttgtattaat tctggagcaa gtaaagatga aattatagaa gctataaaaa
     1801 taggtgtaat tggtggcggg tctattacat atccaaacgc aagatttgca ataaaaattt
     1861 tagaagaatt taatatataa ttttgataga cgtagtttat ttagactaat aataatagtc
     1921 tatttaagtc tttgaaatta agctttattt tctaagttca cttcttatta agtttttaga
     1981 attgcatata taaatataaa tttttaaata atatagtagt tttaaattat tt
//
LOCUS       DAMJYR010000263         2016 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.268, whole genome shotgun sequence.
ACCESSION   DAMJYR010000263 DAMJYR010000000
VERSION     DAMJYR010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2016)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2016)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2016
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.268"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            169..783
                     /locus_tag="PC471_003834"
     CDS             169..783
                     /locus_tag="PC471_003834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HDF2790910.1"
                     /translation="MEEVIAKIITIENGFKEIENIAKKIVKNNDSKKCYYLSIYLYRS
                     EYYQVRELAVFILGFISVTISEALLFLKDNVSKDENWRVQEILAKSFDYYCSEVGYEK
                     ALPVIKEWLNSDSPNIKRAVTEGLRIWTSRDYFKSNPDVAISLLSSLKEDDSEYLRKS
                     VGNALRDISKKYPDLVKKELDSWDISNKKVEFVYKLASKCILAK"
     gene            801..1163
                     /locus_tag="PC471_003835"
     CDS             801..1163
                     /locus_tag="PC471_003835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2790911.1"
                     /translation="MKLRDEYTCPLELTHDMIKGKWKSIILWRLRIGPTSLSKLEKEI
                     NGINQKMLLEQLKELIEFGLVDKKTYEGYPLKVEYSLTPIRGKQILDALAIMQDIGIS
                     FMLDNGMEDILKEKGVLK"
     gene            1269..1766
                     /locus_tag="PC471_003836"
     CDS             1269..1766
                     /locus_tag="PC471_003836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HDF2790912.1"
                     /translation="MKVTSTGIVNGVIEDKYGKRGTQFNEGGMPTYSLPLKFEDAPKN
                     TVSFAVFLEDKDSVPVCGFAWIHWLAVNITKDELNENESVTATDFVQGTTSWHGKLGG
                     LDRLEASVYGGMAPPDRPHVYEIHVFALDTILDLEKGFYMNEFFKAIEGHVLDTFTLK
                     GSYSN"
BASE COUNT          762 a          222 c          336 g          696 t
ORIGIN      
        1 taaatttata tgttaaatga ggttgatttt ttattatttt ttatttatgc tagtactaaa
       61 gacttgcaaa cgttttttga caagtcttta ttttgtctaa attaaacaaa cttttttaca
      121 aataaataca taaaatttaa actaatctat ttattggagg agataaaatt ggaagaagta
      181 attgcaaaaa ttattactat agaaaatgga ttcaaagaaa ttgaaaacat tgctaaaaaa
      241 attgtaaaaa ataatgactc aaaaaagtgt tattatctat caatctactt atataggtca
      301 gaatattatc aagtaagaga attggctgtg tttatacttg gttttatatc agtaactatt
      361 tctgaagctt tgttattttt aaaagataat gtgagtaaag atgaaaactg gagagtacaa
      421 gaaatactag ctaaatcatt tgattattat tgcagtgaag taggatatga aaaggctctt
      481 cctgttataa aagaatggct taattctgat agtccaaaca taaaaagggc tgtgacagaa
      541 ggtcttcgaa tttggactag tagagactat tttaaatcta atcctgacgt tgctatatca
      601 ctactttcta gtttaaaaga agatgatagt gaatacttga gaaagtctgt aggtaatgct
      661 cttagagata taagtaaaaa atatccagat ttagtaaaaa aagaattaga ttcatgggat
      721 atatcaaata aaaaagttga gtttgtatat aagcttgctt caaaatgtat cttggcaaag
      781 taaaatggag gtgaacttat ttgaaacttc gtgatgagta tacttgccca ttagaactaa
      841 cacatgatat gataaaaggc aaatggaaat ctattatatt atggagatta cgcataggac
      901 caacttcctt atcaaaactt gaaaaggaaa tcaatggtat taatcaaaag atgttacttg
      961 aacaacttaa agaattaatt gaatttggtc ttgtagataa aaagacatat gaaggttacc
     1021 cacttaaagt tgaatactca cttacaccta ttcgagggaa acaaatattg gatgctttgg
     1081 ctataatgca agatatagga attagcttta tgttggataa tggtatggaa gatattctta
     1141 aggaaaaagg tgttttaaaa taaatagata cccacaaaaa agtaagtaat ttaccattat
     1201 tttcaactgg tatataatta aattatagat atattctatt aatttattat aataggaggt
     1261 aaattattat gaaagtaact agtacaggta tagtaaatgg tgttatagaa gataaatacg
     1321 gtaaaagagg aactcagttc aatgaaggtg gtatgcctac atattcacta cctctaaagt
     1381 ttgaagatgc tccaaagaat acagtatcct ttgctgtatt tttagaagat aaagactctg
     1441 ttcctgtatg tgggtttgca tggatacatt ggttagctgt aaatataaca aaagatgagt
     1501 taaatgaaaa tgaaagtgta acagctactg attttgtaca agggactact agttggcatg
     1561 gcaaactagg aggtcttgac agattagagg ccagtgtata tggtggaatg gctcctccag
     1621 acagacctca tgtctatgaa atacatgtat ttgcacttga tactatcctt gaccttgaaa
     1681 aaggattcta tatgaatgaa tttttcaaag ctattgaagg gcatgtttta gatacattta
     1741 cactaaaagg aagttattct aattaattag gttgactata gctaatcatt aattaaatgc
     1801 tacatttaat tgatgattag cttaattgtt attagaatat tttctgcact ttatcttaat
     1861 atatatttta gttgactggt attttaattt ctattagatt attatcaatt tatataaatt
     1921 gattttagct taaaacttac ttatctattt cagtttctat tagaattatt attagactca
     1981 ttaaaataaa agcactaata taactaataa tatatg
//
LOCUS       DAMJYR010000264         2004 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.269, whole genome shotgun sequence.
ACCESSION   DAMJYR010000264 DAMJYR010000000
VERSION     DAMJYR010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2004)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2004)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2004
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.269"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            142..1353
                     /locus_tag="PC471_003837"
     CDS             142..1353
                     /locus_tag="PC471_003837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HDF2790913.1"
                     /translation="MKGLVLEGGGTKGAYQIGAYKALRELGIEFQGVTGTSIGALNGA
                     YIVQNDIEVMEDIWLNYDYTHFMNIDRETYERFKNVDFTTKNLNAVFELINKARKNEG
                     IDISPLRQLMETTLNEDIIRKSKKDFGLVTVWWDKKINPYPLYIEDIPKGRLIDYLIA
                     SSSLPIFQTNMMDDKLYLDGMFCDNMPIGLLEDKGYEDVVVIRLIDDFIGKMNLNRHQ
                     NINIKTIIPSEYLGGSLNKDRDNVEKNIRLGYLDTMKAYERCTGIKYYFDVEYKYNEE
                     YCFDKFRSMSNETIQYICNLLNIKRDASLRTVMESVIPKLGEVLGLPKDFSYKDLFYC
                     IYEKKLEENNINRIKLYDFNKVIDVVNTNIDSNRNLASNFEVRPKISIKKNKNTKLLT
                     NCIIKDIKNQS"
     gene            1546..1806
                     /locus_tag="PC471_003838"
     CDS             1546..1806
                     /locus_tag="PC471_003838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HDF2790914.1"
                     /translation="MEKVVSIKNASGLHARPAGMFVKKAAEFKSTVEVVAKDKTVNAK
                     SIMGIMSLGLAQGEELKLVVNGEDEEVALAAMVELVESGFGE"
BASE COUNT          844 a          176 c          351 g          633 t
ORIGIN      
        1 tatacttttc aatatttttt tgataaaata agaatgaatt tgtgcaattt tatttttctc
       61 caataactaa aaatagtttt ttgaattaat atttgcttaa gaataaaaat cagaaacgtt
      121 tttgatatag taggtgataa tatgaaagga ttagttttag aaggtggagg aacaaaagga
      181 gcctaccaaa taggtgcata taaagcttta agagagcttg gtatagagtt tcaaggagta
      241 actggcacat caattggtgc tttaaatgga gcatatatag ttcaaaatga tattgaagta
      301 atggaagata tttggttaaa ttatgactat actcatttta tgaatattga tagagaaacc
      361 tatgagaggt ttaaaaatgt agatttcact actaaaaatc tcaatgcagt gtttgagctt
      421 ataaataaag ctagaaaaaa tgaaggtata gatataagcc cattaagaca actaatggag
      481 acaacattaa atgaagacat aataaggaaa tcaaaaaaag actttggact tgttacggtc
      541 tggtgggata aaaaaattaa tccatatcca ttgtatatcg aagatatacc taaaggaaga
      601 ttgattgatt atttaattgc tagttccagt ttgcctattt ttcaaacgaa catgatggat
      661 gataagttgt atcttgatgg aatgttttgt gataacatgc caataggact cttggaagac
      721 aaaggttatg aagatgttgt tgtaattagg ttgattgatg attttattgg gaaaatgaat
      781 ctaaatcgac atcaaaatat aaatataaaa actataatac catcagaata tctaggaggt
      841 tctcttaata aagatagaga taatgtagaa aaaaacatcc gactaggtta cttagatact
      901 atgaaagcat atgaaagatg cactggaatt aagtattatt ttgatgtaga gtataaatat
      961 aatgaagaat attgttttga taaatttaga agtatgagca atgaaacaat acaatatatc
     1021 tgtaatttat tgaatataaa aagagatgct tcacttagaa ccgttatgga aagtgtaata
     1081 ccaaagcttg gtgaggtgct tggattgcca aaggattttt catataaaga tttgttttac
     1141 tgtatatatg aaaagaaact tgaagaaaat aatataaaca ggataaaatt atatgatttt
     1201 aacaaggtta tagatgttgt aaatacaaat attgattcta atagaaattt agcttctaat
     1261 tttgaagtta gacctaaaat tagtattaaa aagaacaaaa acacaaaatt attgacaaat
     1321 tgtattataa aagacataaa aaatcaaagt taaataaaaa acaaaaaaaa atttgtaatt
     1381 atactcgaaa aaagtgtata ttagtagtat aatatttttg tagcactagt gggaacaaaa
     1441 aatgaccata ggacaaaaaa gtaccactta gtaccattaa agtaaaaaac aggaaaacaa
     1501 ttttaaaaaa tataaaatgt aaagtttata ggaggattta aaaaaatgga aaaagtagtt
     1561 agtatcaaaa atgcaagtgg attacatgct agaccagcag gaatgtttgt taagaaggca
     1621 gctgaattta aatcaacagt agaggttgta gcaaaagaca aaactgtaaa tgcaaaatca
     1681 ataatgggaa taatgagttt aggtttagct caaggagaag aattaaagtt agttgtaaat
     1741 ggagaagatg aagaagtagc tttagctgca atggttgaat tagttgaaag tggatttgga
     1801 gaatagatat aataattttt taaaaatttg atatttagaa tctaatatta cttaaagtaa
     1861 agtaacagag aagtattaaa catatacaga ccaacatttg tagatattta atttctttgt
     1921 tactttcatg tttatttaat cagaagatta agactattgt taattgggta taaaaatata
     1981 aatattttag aaaatgaaat aaga
//
LOCUS       DAMJYR010000265         1980 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.270, whole genome shotgun sequence.
ACCESSION   DAMJYR010000265 DAMJYR010000000
VERSION     DAMJYR010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1980)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1980)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1980
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.270"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            165..1076
                     /locus_tag="PC471_003839"
     CDS             165..1076
                     /locus_tag="PC471_003839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YafY family transcriptional regulator"
                     /protein_id="HDF2790915.1"
                     /translation="MKINRLTEIIVILLNKKLVTAKELADRFEVSTRTIYRDIETLSM
                     SGVPVYMTKGKGGGISLIEEYSIDKAILSKKDKESLIVALKTLQATKYPEINSVVNKI
                     GSIFGEQNFSNWIEIDFTEWGSNFNEDDKFTKIKEAILRRNTINFNYVNSLSSQTNRT
                     VEPLKLMYKSKTWYLYGFCKLKDDFRIFRISRIRNLSIKDEVFSRKIIEEVCLNDSKV
                     IKENTITLKLRFKEKMLFRVFDDFNKDLITKNEDDTYDVITEFPIGEWIYGYILSFGD
                     NVEVLEPKDVRDNVITRLRELSKIYSL"
     gene            1251..1748
                     /locus_tag="PC471_003840"
     CDS             1251..1748
                     /locus_tag="PC471_003840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="effector binding domain-containing protein"
                     /protein_id="HDF2790916.1"
                     /translation="MNYEIVEINEKIVIGISKETTNKDGQAVNDIGELWKKFMGKGIY
                     NAIKGKKNDKTIGLYTDYQGDFTSPYSFVACCEVNSNSDKEKNLEELNTNNTVGESII
                     SKVIPAGKYAKFVIVGGQKEVGDFWFEFWQMDFDRTYISDFEEYQCNTFDTKKQEIHI
                     YIGIK"
BASE COUNT          832 a          141 c          349 g          658 t
ORIGIN      
        1 aataaataaa ttaagatgat aatatccaaa gtgagaaaga taatagaaag taaataaaat
       61 catgtaaaaa taatttagat aagattctaa gattttattt atttaaaaat tagtatgatt
      121 aatttagatt aaataaattt aataatttta gtaggtggta atgaatgaaa ataaatagat
      181 taacagaaat aatagtaatt ctattaaata aaaaattagt aacagctaaa gagttggcag
      241 atagatttga agtttctaca agaaccatat atagagatat agaaactctg tctatgtcgg
      301 gagtaccagt atatatgacg aaaggcaaag gaggagggat atctttaata gaagaatatt
      361 ctatagacaa ggctatattg tcaaaaaaag ataaagagag tttaatagtt gcactgaaaa
      421 cattacaggc gactaagtat ccagaaataa attcagtagt aaataaaatt ggctcaatat
      481 ttggagagca gaatttttca aactggattg aaatagactt tactgaatgg ggaagtaatt
      541 ttaatgaaga tgataaattt acaaaaataa aagaagctat tttaaggcgt aatactataa
      601 attttaatta tgtaaatagt ttgtcaagcc agacaaacag aactgtcgaa ccactgaagc
      661 ttatgtataa gagtaagact tggtacttgt atggattttg taaactaaaa gatgatttta
      721 ggatatttag aattagtagg ataaggaatt tatctataaa ggatgaagta ttttcaagaa
      781 agataataga agaagtatgt ttgaatgatt ctaaagtaat taaggaaaat actataacgt
      841 tgaaattgag gtttaaagaa aagatgcttt ttagagtatt tgatgatttt aataaggatt
      901 taataactaa aaatgaagat gatacatatg atgtaattac agaatttcct attggtgaat
      961 ggatttatgg atatatttta tcatttggtg acaatgttga agtacttgaa cctaaagatg
     1021 ttagggataa tgtaataact agactaaggg agttgtctaa aatttattca ttataatata
     1081 atgctagatt aaagatttta ttaaatatat aatatcttgc atttagtata taatatctca
     1141 ctgagtttaa ttatattaaa aatatgacat aaaggtgtcg tatattataa attataattg
     1201 aattagttca aaataaaata gattaataaa aacaaagtga ggtataaagt atgaattatg
     1261 aaatagtaga gataaatgag aaaatagtta taggaatatc gaaggagact acaaataaag
     1321 atggacaagc ggtaaatgat ataggtgaat tgtggaaaaa atttatgggc aaagggatat
     1381 ataatgctat aaagggtaaa aagaatgaca agacaatagg tctatataca gattaccagg
     1441 gtgattttac aagtccgtat agctttgttg catgttgtga agtaaatagt aattctgata
     1501 aagagaagaa tttagaagaa ttaaacacta ataatactgt tggagaaagt attattagta
     1561 aagttatacc agcaggtaag tatgcaaagt ttgttatagt aggaggtcaa aaagaagtag
     1621 gagatttttg gtttgagttt tggcaaatgg attttgatag gacttatata agtgattttg
     1681 aagagtatca atgtaatact tttgatacaa agaaacaaga aattcatatt tacataggaa
     1741 taaagtagat ttgttattac tatattgaat gaaatattga gtatagtaca tttattatag
     1801 atagttttat tatttataac tataagcagt aaatctgtaa aagatttact gcttttttag
     1861 taattaatag tatcagtatc actttgaatc ttcagacata ttcttcattt aatattgttt
     1921 attgataaat gatacttgta aatataatga tttgttattt aatttaaaat atgaaaaaaa
//
LOCUS       DAMJYR010000266         1925 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.271, whole genome shotgun sequence.
ACCESSION   DAMJYR010000266 DAMJYR010000000
VERSION     DAMJYR010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1925)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1925)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1925
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.271"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            119..235
                     /gene="rrf"
                     /locus_tag="PC471_003841"
     rRNA            119..235
                     /gene="rrf"
                     /locus_tag="PC471_003841"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            242..316
                     /locus_tag="PC471_003842"
     tRNA            242..316
                     /locus_tag="PC471_003842"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:274..276,aa:Asn,seq:gtt)
     gene            321..395
                     /locus_tag="PC471_003843"
     tRNA            321..395
                     /locus_tag="PC471_003843"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:354..356,aa:Glu,seq:ttc)
     gene            401..476
                     /locus_tag="PC471_003844"
     tRNA            401..476
                     /locus_tag="PC471_003844"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:434..436,aa:Val,seq:tac)
     gene            482..558
                     /locus_tag="PC471_003845"
     tRNA            482..558
                     /locus_tag="PC471_003845"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:516..518,aa:Asp,seq:gtc)
     gene            569..643
                     /locus_tag="PC471_003846"
     tRNA            569..643
                     /locus_tag="PC471_003846"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:601..603,aa:Thr,seq:tgt)
     gene            658..742
                     /locus_tag="PC471_003847"
     tRNA            658..742
                     /locus_tag="PC471_003847"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:692..694,aa:Tyr,seq:gta)
     gene            752..825
                     /locus_tag="PC471_003848"
     tRNA            752..825
                     /locus_tag="PC471_003848"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:784..786,aa:Gly,seq:tcc)
     gene            836..912
                     /locus_tag="PC471_003849"
     tRNA            836..912
                     /locus_tag="PC471_003849"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:870..872,aa:Arg,seq:tct)
     gene            922..997
                     /locus_tag="PC471_003850"
     tRNA            922..997
                     /locus_tag="PC471_003850"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:955..957,aa:Gln,seq:ttg)
     gene            1009..1084
                     /locus_tag="PC471_003851"
     tRNA            1009..1084
                     /locus_tag="PC471_003851"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1042..1044,aa:Lys,seq:ttt)
     gene            1087..1175
                     /locus_tag="PC471_003852"
     tRNA            1087..1175
                     /locus_tag="PC471_003852"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1121..1123,aa:Ser,seq:tga)
     gene            1193..1283
                     /locus_tag="PC471_003853"
     tRNA            1193..1283
                     /locus_tag="PC471_003853"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1227..1229,aa:Ser,seq:gct)
     gene            1292..1368
                     /locus_tag="PC471_003854"
     tRNA            1292..1368
                     /locus_tag="PC471_003854"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1326..1328,aa:Pro,seq:tgg)
     gene            1454..1529
                     /locus_tag="PC471_003855"
     tRNA            1454..1529
                     /locus_tag="PC471_003855"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1487..1489,aa:Trp,seq:cca)
     gene            1590..1666
                     /locus_tag="PC471_003856"
     tRNA            1590..1666
                     /locus_tag="PC471_003856"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1624..1626,aa:Pro,seq:tgg)
     gene            1673..1749
                     /locus_tag="PC471_003857"
     tRNA            1673..1749
                     /locus_tag="PC471_003857"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1707..1709,aa:Ile,seq:gat)
     gene            1753..1828
                     /locus_tag="PC471_003858"
     tRNA            1753..1828
                     /locus_tag="PC471_003858"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1786..1788,aa:Phe,seq:gaa)
     gene            1836..1912
                     /locus_tag="PC471_003859"
     tRNA            1836..1912
                     /locus_tag="PC471_003859"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1870..1872,aa:Met,seq:cat)
BASE COUNT          484 a          442 c          524 g          475 t
ORIGIN      
        1 attcattaag atatatgtgc agtttttaga gtgttaactc taaaaaaaaa taaaaaaagt
       61 attgacaaaa atagtaaaaa atgttaatat tatacttgtc ctagcaacaa gaaaaaaatg
      121 tggttataat agcggagaag atacacctgt ttccattccg aacacagaag ttaagttctc
      181 tagcgctgat ggtacttggt gggaaactgc ctgggagagt aggacatagc cacgtgaatg
      241 ttccaaggta gctcaacggt ggagcaaccg gctgttaacc ggtaggctgt gggttcgagc
      301 cccacccttg gagccattat ggctcgttgg tcaagaggtt aagacaccgc cctttcacgg
      361 cggtaacagg ggttcaattc ccctacgagt caccatttgt gggatcatag ctcagctggg
      421 agagcacctg ccttacaagc agggggtcac aggttcgagc cctgttggtc ccaccatttg
      481 cggcctggta gttcagttgg ttagaatgcc agcctgtcac gctggaggtc gagggttcga
      541 gccccttcca ggtcgccaaa ataaaaatgc tggtgtggct caacggtaga gcagctgact
      601 tgtaatcagc aggttgtagg ttcgattcct atcaccagct ccatgtaaaa ctaatacgga
      661 ggatttcccg agcggccaaa gggggcagac tgtaaatctg ttgtcatcga cttcggtggt
      721 tcgaatccac catcctccac caattaaata tgcgggtgta gctcaatggt agagttctgg
      781 ccttccaagc cagctgtgag ggttcgatcc ccttcacccg ctccaaagaa agcgtgggtg
      841 catagctcag ctggatagag caacgccctt ctaaggcgtg tgtccggggt tcgaatccct
      901 gtgcgctcac cacatatata ttggggatta gccaagtcgg taaggcacat gactttgact
      961 catgtatgcg taggttcgag tcctgcatcc ccagccaaat aaaaatatgg tctattagct
     1021 cagtcggtag agcacctgac ttttaatcag ggtgtcccgc gttcgagtcg cggatagatc
     1081 accaatggag aggtgtccga gtggtttaag gagctggtct tgaaaaccag tgacttcgaa
     1141 aggggccgtg ggttcgaatc ccaccctctc cgccaaaaaa atgactgaat ttggagaagt
     1201 actcaagtgg ctcaagagga tcccctgcta agggattagg ctgggtaacc ggtgcgaggg
     1261 ttcgaatccc tccttctccg ccattcaaaa acgaggtgta gcgcagtttg gtagcgcaca
     1321 tggtttggga ccatggggcc gggggttcga gtcccttcac ctcgaccaac ttaagctttt
     1381 agttcagttg gttagagtgc ccaactcatg gctagtaatt ctagggtttg agtccatgaa
     1441 ggttattctt cataggggtg tagctcagtt ggtagagcgt cggtctccaa aaccgtgcgc
     1501 cgggggttcg agtccctcca cccctgccaa ataaaaagga cttgaaaaaa gttggatttt
     1561 agagtagtat aagatagtaa ataagatttc gaggtgtagc gcagtttggt agcgcacatg
     1621 gtttgggacc atggggccgg gggttcgagt cccttcacct cgaccaagaa aagggcctat
     1681 agctcaggtg gttagagcgc acgcctgata agcgtgaggt cgctggttcg agtccagcta
     1741 ggcccaccat ttgcccagat agctcagtcg gtagagcagg ggactgaaaa tccccgtgtc
     1801 ggtggttcga ttccgcctct gggcaccagg aatacggcgg tatagcttag ttggctagag
     1861 cgttcggttc atacccgaaa ggtcataggt tcgactccta ttaccgctac catctaacta
     1921 tatca
//
LOCUS       DAMJYR010000267         1881 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.272, whole genome shotgun sequence.
ACCESSION   DAMJYR010000267 DAMJYR010000000
VERSION     DAMJYR010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1881)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1881)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1881
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.272"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            111..395
                     /locus_tag="PC471_003860"
     CDS             111..395
                     /locus_tag="PC471_003860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2790917.1"
                     /translation="MLVYNKSFYPNDIFPRLDFSKIKKQLKLIDNDLSDFGSICIIEK
                     EHYTISVNSIGEINVYYDLEYENKVYRIVYEIEKLFKSQVGRFSISTYRN"
     gene            complement(1166..1381)
                     /locus_tag="PC471_003861"
     CDS             complement(1166..1381)
                     /locus_tag="PC471_003861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2790918.1"
                     /translation="MAIVVNLDVMMAKRKISLKDLAEKIDITNANLSILKTGKAKAIR
                     FTTLNEICKALDCQPGDILEYVEDEND"
     gene            complement(1392..1859)
                     /locus_tag="PC471_003862"
     CDS             complement(1392..1859)
                     /locus_tag="PC471_003862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HDF2790919.1"
                     /translation="MITKFFSNILSVFLYFLLSLLGVAGIIFLCMIPMFLYDFSSDKV
                     IALIARILYFSDYFIVILFLIFIINSTKYSPFVLENVKRFKVMGFCLLINTIIECING
                     YNLNTNNIRIFGTDSGGISPLMIISFIFALMCFVIAETFDKAIKIKEDNDLTI"
BASE COUNT          759 a          202 c          235 g          685 t
ORIGIN      
        1 tttgtatttt gttttatata aagtaatttg aagaatatgg aaaaacttga ttagtacatt
       61 attaattagg gtataaaatg gtgtaaatag aatgataagg ggtggatgaa atgcttgtat
      121 ataataaaag tttttatcct aatgacatat ttccaagatt agatttttca aaaataaaaa
      181 aacagttaaa attgatagat aatgacctgt cagattttgg aagcatatgt ataatagaaa
      241 aagaacatta tacaataagt gtaaacagta taggtgaaat aaatgtgtat tatgatttag
      301 agtacgaaaa taaggtgtat agaatagttt atgagattga aaagttattt aaatctcaag
      361 ttggaaggtt tagcatatct acatacagaa attgataatt aaaaaagtag aaattaaaaa
      421 acttaatact aaagatatag ataaatatat gaggtaacag ctagagagag ttgttaccct
      481 ttttaaatac taagtatata ttattcttat taattttttg taaattcact aaaagacctt
      541 tttatagaat attattttct ttttttatta aataggtaaa tcagtaaaaa tgcaattcca
      601 gttccaatta ttattccaat gatattttct ataattaatt tttttaatat aaaagtaaat
      661 aataaaaatg ctatacataa acctaaaata tatcctttaa aataggttga aagttttctt
      721 ttcatataat caccaacttg tatcagattt agtttactta attagagtat aacagtgttg
      781 taacttcaaa gagtatctaa aagattacaa atgttagata aaatcatcaa taatgtaatt
      841 tataacacgt agctataaac tatgcaataa tataaatgga ggtataagta aatgaaaaag
      901 ttccatgttt tgctaacagt ttaaataaat attaggtaga aaagaatgta tataggaatc
      961 tgtggtaaaa ttatcaattt aaaagtatta tttagtgtga tacagttctt ataggtaatg
     1021 gataacaaat agataaagga acagtttctt tcttagaaca tattttgtta tgactttaaa
     1081 caaagttaat attaatgtca ataatatagt aaaagaataa aaatcatata gtataataaa
     1141 aaagagtgtt tctaaacact cttttttaat cattttcatc ttctacatat tcaagtatat
     1201 cacctggctg acagtctaaa gctttgcaaa tttcgtttag agtggtaaat ctaatagctt
     1261 ttgcttttcc agtttttaaa attgataaat ttgcatttgt aatatctatt ttttctgcca
     1321 aatctttaag tgatattttc ctttttgcca tcattacatc taggttaact actattgcca
     1381 taacatcacc tctatatcgt taaatcatta tcttctttta tttttatagc tttatcaaat
     1441 gtttctgcaa ttacaaagca cattagtgca aatataaaag agataatcat aagtggtgag
     1501 ataccgccac tatcagttcc aaaaattctt atattattag tatttaaatt ataaccattg
     1561 atacattcaa ttatagtgtt tatcaataaa caaaatccca ttaccttaaa cctttttaca
     1621 ttttcaagta cgaatgggga atacttagta ctattaatta taaatatcaa gaataagatg
     1681 actataaagt agtcagaaaa atatagtatt cttgcaatta aagcgattac tttgtcagaa
     1741 ctaaaatcat ataaaaacat aggtatcata cataagaata ttataccagc aactcctaaa
     1801 agtgataata gaaaatatag aaatactgat aatatattac taaaaaattt tgtaatcata
     1861 aaatatcatc tcctttatat a
//
LOCUS       DAMJYR010000268         1824 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.273, whole genome shotgun sequence.
ACCESSION   DAMJYR010000268 DAMJYR010000000
VERSION     DAMJYR010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1824)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1824)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1824
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.273"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            165..1493
                     /locus_tag="PC471_003863"
     CDS             165..1493
                     /locus_tag="PC471_003863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDF2790920.1"
                     /translation="MGSNELKKTLGVSAALSTVVGSVIGAGVFFKPQAVYTLTGGAPG
                     LGILAWLIAGIITITAGLTAAEVSVAIPKTGGMMVYIKEIYGEKLGFLTGWMQIVLFY
                     PGMMAALGVIFGEQASALIGSPSLLLPIAIGIIIIVAGLNMLGSKTGGVIQTVSTICK
                     LIPLILIMVVGFIKGGGNNPILTPMVGEGLSLGSVLGQVLIAILFAFDGWMNVGTLAG
                     EMKNPGKDLPKAIIGGLSVVMAVYFIINLAYLWVLPANELANYASPASAVAEVIFGSM
                     GGKIISVGILISVFGALNGFLLTGSRVAYTLATDKTLPKYGIFSKLNSAQVPANAIAL
                     VSVIASIYALSGQFNLLTDLAVFATWIFYVLTFIGVMKLRKTHPNIPREYKVPLYPIV
                     PIIAIASGIFVVVNQLCFAGMKTTMISIGGLVITAIGLPVYAYMTRGVKR"
BASE COUNT          625 a          208 c          328 g          663 t
ORIGIN      
        1 aattttttta tatttaaaca taactaaaaa taaaagatta ctaaagctaa gtagagttta
       61 ttttatttta ataaataata aagctatttt tctatatatt ggcataatat atgcttattt
      121 aactatgaaa ttaaaattaa ataggattaa tgaggaggaa aaaaatggga agtaatgaac
      181 tgaaaaagac tcttggcgta tcagcagcat tatcaactgt tgttggttcc gttataggag
      241 cgggcgtatt ttttaaacca caagcagtct atacattaac tggaggagct ccaggtcttg
      301 gtatattggc atggttaata gctggaatca taactataac agctggtctt acagctgcag
      361 aggtatcagt tgcgatacca aaaactggtg gaatgatggt atatataaaa gaaatttatg
      421 gagaaaaatt aggtttttta actggatgga tgcaaattgt attattttat ccagggatga
      481 tggcagcatt aggtgttata tttggagaac aagcatcggc actaattggt agtccatcac
      541 ttttacttcc aatagcaatt ggaattataa taatagttgc agggcttaat atgttaggtt
      601 caaaaactgg aggagtcata caaactgttt ctacaatttg taagttaata ccacttattt
      661 taataatggt tgttggattt attaaaggtg gtggaaataa tccaatactt acacctatgg
      721 ttggtgaagg attaagcctg ggtagtgtac taggtcaagt tttaattgct atattatttg
      781 cttttgatgg atggatgaat gttggtactt tagctggaga aatgaagaat cctggaaaag
      841 accttccaaa agctataata ggtggattgt cagttgttat ggctgtttat tttataataa
      901 atttagcgta tctttgggta ttaccagcta atgaattagc aaattatgct tctcctgcat
      961 cagcagttgc tgaagttata tttggttcaa tgggtggaaa aataatatct gtaggtatat
     1021 taatttcagt gtttggagca ttaaatggat ttttactaac tggttcaagg gtggcatata
     1081 ctttagctac agataagact ttaccaaagt atggtatatt ttctaaatta aattctgctc
     1141 aagttccagc aaatgcaata gcattagtat ctgttattgc atctatatat gctttgtcag
     1201 gtcaatttaa tttattaact gacttagctg tatttgctac ttggatattc tatgtattaa
     1261 cttttatagg ggttatgaaa cttagaaaga ctcatccaaa tataccaaga gagtataaag
     1321 ttccattata tcctattgtt cctataatag ctatagcaag tggtatattt gttgtagtta
     1381 atcaattgtg ttttgcaggt atgaaaacta ctatgatatc tataggaggt cttgtaataa
     1441 cagctatagg cttaccagtg tatgcttata tgactagagg agtaaaaaga taaaccaagt
     1501 agtttacaaa tgcttcataa ttaaaataaa agagactgtc tcaaatagag ataagtctct
     1561 tttttattgt taaaatggta aagatatgtt taattttaca aaaaaagagc gttccatgaa
     1621 ctaaaaagtt tattttaagg tattcatttt atttttagtt ttttgattat aaaattaaac
     1681 atctaaaaaa ttatttattt taaaatactt tattttaaag tgttttttat caaactaagt
     1741 ggtatctgta caacaaatat tatacatagc ttttatgaaa attaaatctt tctgttatat
     1801 ataatagaat attgtattat atag
//
LOCUS       DAMJYR010000269         1780 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.274, whole genome shotgun sequence.
ACCESSION   DAMJYR010000269 DAMJYR010000000
VERSION     DAMJYR010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1780)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1780)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1780
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.274"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            86..1324
                     /locus_tag="PC471_003864"
     CDS             86..1324
                     /locus_tag="PC471_003864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conserved phage C-terminal domain-containing
                     protein"
                     /protein_id="HDF2790921.1"
                     /translation="MNKGFYKLNSVVLNFDIKNDVNKIRFNHYIMRKENIQRLQGTLP
                     NGQFYMTVRRAATDLQLSISTISRLVKEFLDLGIIRLVSKGVKGNRSVYSYVSIEFNG
                     SLFDDSKYMELDCVCNDEFIDNSGIEFDCDSKDNSFNGDINVETVHCINEYFSDDDVE
                     HNYNNFHNKDTIKDKAISNISTIKKYIDESFDTNKSENSNLQQESINFNFNDTCNEID
                     KRTNNSIENNQNKRPMRLSDISLLSDKGMTNKKNYFNHNHGIQKGTKSVTKKKESLNK
                     FNKKNIQQSIINMLNKKSGKNFKIDSPISIKLINERLKEGYVLEDFYKVIEVKVAKWK
                     NTIMEMYIRPETLFSYKFESYVNENISMKSQSNVHPCKEGNSNDSSNYIHSDYITYDG
                     SVRERKKYENVRYWNPICEI"
BASE COUNT          755 a          151 c          295 g          579 t
ORIGIN      
        1 tatttatatt taaactattt tgttatttat ataaaaatac ttatctagtt actagcaatt
       61 attagtagaa atgggggaaa tagagatgaa taagggattt tacaaattaa attcagtggt
      121 attgaacttt gatattaaga atgatgttaa caagataaga tttaatcatt atataatgag
      181 aaaagaaaac atacaaagac ttcaaggaac tctgccaaac ggacaatttt atatgacagt
      241 gagaagagct gctacagatt tacaactttc tatttctact ataagtaggc ttgtaaaaga
      301 gtttttggat ttagggataa taagattagt gtctaagggt gtaaaaggta atcgttctgt
      361 atatagttac gtaagtatag agtttaatgg aagtttattt gatgatagta aatatatgga
      421 acttgactgt gtatgtaatg atgaatttat tgataatagt ggcatagagt ttgattgtga
      481 tagcaaagat aattctttta atggagatat aaatgtagaa actgtgcatt gcattaatga
      541 gtatttctct gatgatgatg tagagcataa ttataacaat ttccataaca aagatactat
      601 aaaggataaa gccatttcta atattagcac tatcaaaaaa tatattgatg aatcttttga
      661 tacaaataaa tcagaaaatt ctaacttaca acaggagtca ataaatttca actttaatga
      721 tacatgcaat gaaatagata aaagaacgaa taatagtata gaaaataatc aaaataaaag
      781 gcctatgaga ttgagtgaca tcagtctttt aagtgataaa ggtatgacaa ataaaaagaa
      841 ttactttaat cataatcatg gtatacaaaa agggactaaa tctgtaacaa agaaaaaaga
      901 atccttaaat aaatttaata aaaaaaatat acagcaaagc attataaata tgctaaataa
      961 aaaatcaggt aagaatttta aaatcgattc tccaataagc ataaaactta tcaatgaaag
     1021 gctaaaggaa ggctatgtct tagaagattt ctacaaagtt attgaggtaa aagtggctaa
     1081 gtggaaaaat accataatgg aaatgtatat aagaccagaa actttgttta gctataagtt
     1141 tgaatcctat gtaaatgaaa acatatctat gaaaagtcaa agtaatgttc acccatgtaa
     1201 agaaggcaat agtaatgata gcagtaatta tatacattct gattatataa catatgatgg
     1261 cagtgtgcgt gagagaaaaa aatatgagaa tgtaagatac tggaatccta tatgtgaaat
     1321 ttaattagtt taaaaatgta tttatgataa ataagatatt aatagagaat agtgagctat
     1381 agcaaatgat atatcaatag ttggtagtaa gttatattaa gtaagatatt actatcaggt
     1441 agtgaattat attgaacgat atattaataa ttggcagtaa gttatatcaa ataagatatt
     1501 actatagagt aatgaattat attgaacgat atattaataa ttggcagtaa attatattaa
     1561 ataagatatt actatagagt aatgaattat attggacgat atatcaatag ttggtagtaa
     1621 gttatattaa gcaagatatt actatcaggt agtgaattat attgaacgat atatcaataa
     1681 ttggtagtaa attatattaa gtaagatatt actatcaggt agtgaattat attgaacgat
     1741 atattaataa ttggcagtaa attatattaa ataagatatt
//
LOCUS       DAMJYR010000270         1696 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.275, whole genome shotgun sequence.
ACCESSION   DAMJYR010000270 DAMJYR010000000
VERSION     DAMJYR010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1696)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1696)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1696
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.275"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            <1..1123
                     /locus_tag="PC471_003865"
     CDS             <1..1123
                     /locus_tag="PC471_003865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HDF2790922.1"
                     /translation="ICIFITYVLYKINYVVDKWKSVFISLLYWMLLIGFDSIGLSIVM
                     TLNSIKNMNDLLEDNLLKLELIMISKSLLIVLIPLLKFIRLKTKINKRDCIYLSIPLI
                     ANIIGVIVIFEFIFKDKIIDSKESLIILTISTVFLLSNLSLVSIIGRIIKDNSLKIEY
                     EITKKRMNMQYKYYLNLQEYQLKIRKLYHDMNNHIICIQNVYGKNEFADKYIKDINKQ
                     IKECNTIFNTQNMILDVILSDKKSICDMNNIYFLVDINFLECNFIEMPDICSIFSNII
                     DNAIEACIKIKDTSIQKKIKLRGTVINRLFVIKCENTKINEVVLKNNKVITSKKDSFL
                     HGIGISSVKSSVEKYNGNVEIYSDKNKFTITIYIPLTRN"
BASE COUNT          737 a          111 c          246 g          602 t
ORIGIN      
        1 tatatgtata tttattacat atgtattgta taaaataaat tatgtagtgg ataaatggaa
       61 aagtgtattt ataagtttgc tatattggat gttattaatt ggatttgata gtattggatt
      121 aagtatagta atgacactta attctataaa aaatatgaat gatttattag aggataattt
      181 gttgaagtta gagttaataa tgatatctaa atcattacta atagtattaa taccattgct
      241 taagtttatc agattaaaaa caaaaataaa caagagggat tgtatatatt tatctattcc
      301 attaatagca aacattattg gtgtaatagt aatatttgaa tttattttta aagataagat
      361 tatcgattct aaagagagtt taataatact tacaatatca actgtatttt tactatcaaa
      421 tctttcatta gtaagcataa taggaagaat aataaaagat aatagtttaa agatagaata
      481 tgaaattaca aaaaaaagaa tgaatatgca atataaatat tatttaaatt tacaggaata
      541 tcagttgaaa ataagaaaac tatatcatga tatgaataat catatcattt gtattcagaa
      601 tgtttatggg aaaaatgagt ttgcagataa gtatattaaa gatataaata agcaaataaa
      661 agaatgtaat actatattta atactcagaa tatgattcta gatgttattt taagtgataa
      721 aaaatctatt tgtgatatga ataatatata ttttctagta gatattaatt ttttagaatg
      781 taattttata gagatgccag atatatgtag tatattttca aatataatag ataatgcaat
      841 agaagcttgt attaagatta aggacacaag tatacaaaaa aagattaaat taagaggaac
      901 tgttataaat aggttatttg taataaaatg tgaaaataca aagataaatg aagttgtatt
      961 aaaaaataat aaggttatta caagtaagaa agattccttt ttacatggaa taggaataag
     1021 tagtgtaaaa agttcagtag aaaaatataa tggaaatgtg gaaatatact cagataaaaa
     1081 caagtttaca attactattt atataccatt aactaggaac taattctagt tggggctcta
     1141 aattgcccag ttggagcagt agtattgaaa gataaaatag aagatgctat ctttaaggca
     1201 taggatattt ggaggtgata aaaatggttg aatgctatgc taataagata acatcctttt
     1261 tagtactcaa taaaataata gaacaaaaaa tgtgatttat atttgtttga atttaaaatg
     1321 ttgataggtt ttatagttat attatttgca tgatatatct taaataaatt tagaaaaata
     1381 gagttatttt tatctgcaaa tgcgacctta gtatggtaac attattgacc aaaagttcta
     1441 tatagtataa aaaaataaaa tgtaaatttg ataaacttta taactaggaa cataaaaaca
     1501 acaattaaat aactaattag atagtaaagt tgatattagt aataaatatt aaagaaaaat
     1561 attttactaa tttgaggagg acttagttgt gatagattct gaagaaatga tttttaatgt
     1621 agaagattta attatttata gaaaaaatca actagtaatt atttataata agaaatttag
     1681 aaaatatatt aaattg
//
LOCUS       DAMJYR010000271         1660 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.276, whole genome shotgun sequence.
ACCESSION   DAMJYR010000271 DAMJYR010000000
VERSION     DAMJYR010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1660)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1660)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1660
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.276"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            99..833
                     /locus_tag="PC471_003866"
     CDS             99..833
                     /locus_tag="PC471_003866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDF2790923.1"
                     /translation="MKQNKYDDDRFFNKYSKMERSTNGLAGAGEWHVLKKMLPDFKGK
                     RVLDLGCGFGWHCQYAVENGAISAVGVDISEKMLKEARNKTQLDNINYICMPIEDINF
                     PKDSFDVVISSLAFHYIQSFEDICKNISNCLSNKGNFVFSVEHPIFTAYGSQEWYCDK
                     DGTNLHWPVDGYFEEGVRKSNFLGEEVIKYHKTLTTYLNTLMKTGFEILEIIEPQPEE
                     KLLDTIPDMKDELRRPMMLLVSAKKR"
     gene            complement(976..1467)
                     /locus_tag="PC471_003867"
     CDS             complement(976..1467)
                     /locus_tag="PC471_003867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HDF2790924.1"
                     /translation="MNNQQQEVYKKLDELSIPYEAVNHPPVYTIEEMEELKTLHMEFV
                     VKNLFLRDAKGKEHFLVVLGKDKKADLKDIRQQIDSKPLSFASEERLQKYLKLTKGAV
                     TPFGILNDKEAVVKVVFDKDLLKMDKIGIHPNDNTATVFLKFEDMKKLIEENGNEIYY
                     VEV"
BASE COUNT          618 a          199 c          242 g          601 t
ORIGIN      
        1 tagaaacttt tattatttta tataaaagtg ataaaaactt atattttgta tttaatagag
       61 tgttgctaag tgaatatatt taaaatggag gaaactttat gaaacaaaat aaatatgatg
      121 atgatagatt ttttaataaa tacagtaaaa tggaacgttc tacaaatgga ttagcaggtg
      181 ctggagagtg gcatgtttta aaaaaaatgt tacctgattt caaaggaaaa agagttttag
      241 atttaggatg tggatttgga tggcattgtc aatatgcagt tgagaatgga gcaatttctg
      301 ctgttggagt tgatatttca gaaaaaatgt taaaagaagc tagaaacaaa acgcaacttg
      361 ataatataaa ttacatatgt atgcctattg aagatattaa ttttccaaaa gatagttttg
      421 atgtagttat tagttccttg gcatttcatt atattcaatc atttgaagat atatgtaaga
      481 atataagtaa ttgtctttca aataaaggta attttgtatt ttctgttgaa catcctatat
      541 ttactgcata tggaagtcaa gagtggtact gtgataaaga tggcactaat ctacactggc
      601 cagtagatgg atattttgaa gaaggagtac gtaagtcaaa ttttctagga gaagaggtta
      661 taaaatatca taaaacatta acaacttatt taaatacgtt aatgaaaaca ggatttgaaa
      721 tattagaaat tattgaacct caaccagaag aaaaactgtt agatacaatt cctgatatga
      781 aagatgagct acgtagacct atgatgttac ttgtatctgc aaaaaaaaga taagtaaata
      841 taaataatca ttattgtaga aaaaaagttg tagaaaataa ttacactaat ggagatgatt
      901 ataccagtgg tattaattac accagtagta aggaggcaaa agcattcgtt gcttttgcca
      961 ttttttaagt tgcaattata cttctacgta gtatatttcg tttccatttt cttctataag
     1021 ctttttcata tcttcaaatt ttaaaaatac agtagcagta ttatcatttg gatgtatacc
     1081 tattttatcc atttttaata aatctttatc aaatacaact tttacaacag cctctttatc
     1141 atttaatata ccaaaaggag tcacagctcc tttagtaagt ttcaagtatt tttgaagtct
     1201 ttcttccgat gcaaaactaa gaggtttact atcaatttgt tgacgaatat cttttaaatc
     1261 tgcttttttg tctttaccta atacaactaa aaaatgttct tttcctttag catcacgaag
     1321 aaataggttt tttacaacaa attccatatg taatgttttt aattcttcca tttcttcaat
     1381 tgtataaact ggtggatgat ttacagcctc ataaggtatg cttaactcat ctaatttttt
     1441 atatacttcc tgttgttgat tgttcatgat ttcctcccat tagaaattta ttttatatat
     1501 aaataatatt atcacagaaa actttaaaag aaaacgtttt atataatata gacaatagat
     1561 tatattttta taaagaagca agaatactca ataaaactta aagaaattaa acaatttcta
     1621 aaaatacctt gaaaatagaa ccttatttgc catttataaa
//
LOCUS       DAMJYR010000272         1608 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.277, whole genome shotgun sequence.
ACCESSION   DAMJYR010000272 DAMJYR010000000
VERSION     DAMJYR010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1608)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1608)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1608
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.277"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            134..1570
                     /gene="miaB"
                     /locus_tag="PC471_003868"
     CDS             134..1570
                     /gene="miaB"
                     /locus_tag="PC471_003868"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HDF2790925.1"
                     /translation="MSKRKQVTIPIEKIQEQDKYIEQIHRQNEEYFNRTGKRKLVFTQ
                     TFGCQMNEHDSEKLCSMLEEMGYQMSMMVEESDLIIYNTCAVRENAELKVYGNLGQLK
                     HLKGKNPDMKIAVCGCMMQQPHVVEELRKKYKHVDLIFGTHNLYKFPQLLTESINSDK
                     MLVDVWDVDGEVIEGLRSNRKFELKAFVNIMYGCNNFCTYCIVPYTRGRERSRTPEDI
                     INEIKELVANGTKEITLLGQNVDSYGKTLENPVSFSELLRKVNDIEGIERVRFMTSHP
                     KDISDEVIYAIRDCDKVCEFLHLPIQCGSSSLLKKMNRHYTKEYYLEIIEKAKKEVPG
                     IAFSTDLMIGFPGETEEDLLDTLDVVEKVRYDSAFTFIYSKRQGTPAAKMENQIPEDI
                     KHDRFNRVLEAVNRISAEINDGYKDRIVEVLVEGRSKNNENKFAGRTRQNKLVNFEGG
                     NDDLIGKLVMVKITEPRTFSLNGILVNN"
BASE COUNT          689 a          166 c          293 g          460 t
ORIGIN      
        1 atatagtata tataaatcat tagatttact attaaaacac aactagaaaa ataataatat
       61 ctttaaaaga tttttcttat ttgatataat taaggttatg tgtaatataa gaaattaact
      121 aggaggaata gacgtgagca aaagaaaaca agtaacaata cctatagaga aaatacaaga
      181 acaagataaa tatatagaac aaatacacag acaaaatgaa gaatacttca acagaactgg
      241 aaagagaaaa ctcgtgttta ctcaaacctt tgggtgtcaa atgaatgaac atgattcaga
      301 aaaattatgt tcaatgttag aagagatggg ataccaaatg tcaatgatgg ttgaagaaag
      361 tgatttaata atatataaca cctgtgcagt tagagaaaat gcagagctaa aagtatatgg
      421 taacttagga caattaaaac atctaaaagg aaaaaatcca gatatgaaaa tagctgtatg
      481 tggatgtatg atgcaacaac cacatgttgt agaggaatta agaaaaaaat ataaacatgt
      541 agaccttata tttggaacac ataatctata taaatttccg caattactta cagaatcaat
      601 aaattctgac aaaatgttag tggatgtatg ggatgtggat ggagaagtaa tagaaggtct
      661 tagaagtaat agaaaatttg aattaaaagc atttgtaaat ataatgtatg gatgcaataa
      721 tttctgcaca tattgtatag tcccatacac aagaggaaga gaaagaagta gaactcctga
      781 agatataatt aacgagataa aagagcttgt tgcaaatgga acgaaagaaa tcactttact
      841 tggacaaaat gtagattctt atggtaagac acttgaaaat ccagtttcat tctcagaact
      901 tttaagaaaa gtgaatgata tagaaggaat tgaaagggtt cgatttatga catctcatcc
      961 aaaagatata tctgatgaag taatatacgc aattagagat tgtgataagg tgtgtgagtt
     1021 tttacatcta ccaatacaat gcggaagcag tagccttttg aaaaaaatga atagacatta
     1081 tacaaaggaa tattatttag aaataataga aaaagctaaa aaggaagttc caggtattgc
     1141 tttttctaca gacttaatga ttggtttccc aggagaaaca gaagaagatt tattggacac
     1201 tttagatgtg gttgaaaaag ttagatatga ttcagctttt acatttattt attctaaaag
     1261 acagggaact cctgctgcta aaatggaaaa tcaaatacca gaagatataa agcatgatag
     1321 atttaatagg gtgttagaag ctgtaaatag gatttctgca gaaataaatg atggatataa
     1381 agatagaata gttgaagttc tagttgaagg aagaagtaaa aataatgaaa ataagtttgc
     1441 tggtagaact agacaaaata aactcgtaaa tttcgaaggt ggaaatgatg atttaattgg
     1501 taaattagtt atggtcaaaa ttactgaacc tagaactttt tcattaaatg ggatattagt
     1561 aaataattaa tattgtacaa aataataaaa ttatgtaaac taatcaaa
//
LOCUS       DAMJYR010000273         1558 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.278, whole genome shotgun sequence.
ACCESSION   DAMJYR010000273 DAMJYR010000000
VERSION     DAMJYR010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1558)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1558)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1558
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.278"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            52..1488
                     /locus_tag="PC471_003869"
     CDS             52..1488
                     /locus_tag="PC471_003869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp28"
                     /protein_id="HDF2790926.1"
                     /translation="MKFYKRILTLTLSISFVFANIQLVDAISSVEKIQGKNKYEIAGK
                     IADKNAYKTAILINTSNSIADGLSASGLAGALNAPILLTEKNTIPTETSARLKNVSKV
                     YIIGGTYSISTSVENSLKSKKMKVVRIKGNDRIKTSYNVAKEINSIKKVNTVMLTNAY
                     KGEADAISIASVAARDKAPIILTNGQSIPFSTSGLKSYVIGGTASMSTTLVNNTKSTR
                     LGGSTRFETNKAIINKFYKDAREFYIAGAYELTNALVGSSLSKHGPMVLVNDGSNKSI
                     LKNAKKITSIGYIDSNIVQQCLNITNGIGDINTGVVKNVKPTTKTIKDGMYKVGKDIS
                     AGEYLITSNSGSYASYYEVTSDSTGNADSILSNDIFSGTRYITLKNGQYIKIEDSTMT
                     LAKYAKAQKAKNGKFGNGMYKIGLEIPAGEYIIMSNSSDAYYEVRNDSLGNAEGIVTN
                     DTFSGRRYITVEEGQYLILNDCYLIENE"
BASE COUNT          637 a          161 c          270 g          490 t
ORIGIN      
        1 agaatatgta tttatatatt aaaaatttat tatgaggggg acaattttat tatgaaattc
       61 tataaaagaa tattaacatt gactttaagt atttcttttg tatttgcaaa tattcaatta
      121 gtagatgcaa tatcatctgt agaaaaaata caaggaaaaa ataagtatga aatcgcaggt
      181 aaaatagcag ataaaaatgc atataaaact gctatattaa ttaatactag taactctatt
      241 gcagatggat taagtgcaag tggactagca ggagctttaa atgcccctat actattaact
      301 gaaaaaaata ctataccaac tgagacatct gcaagattaa aaaatgtatc aaaagtttat
      361 ataattggag gaacatattc tattagtaca tctgtcgaaa attcattaaa atctaagaaa
      421 atgaaagttg taagaattaa gggaaatgac agaataaaaa caagttataa tgttgcaaaa
      481 gaaataaatt caataaaaaa agtcaatact gttatgctaa ccaatgcata taaaggagaa
      541 gcagatgcta taagtattgc atctgtagca gcaagagata aagcaccaat aattcttact
      601 aatggacaaa gtataccttt ttcaactagt ggtttaaagt cttatgtaat aggaggaaca
      661 gcttcaatga gtaccacttt agtaaacaat actaaatcta ctagactagg ggggagtact
      721 cgttttgaaa ctaataaagc aataataaat aagttttata aggatgctag agagttttat
      781 atagctggag cttatgaatt aacaaatgca cttgtaggat cttctttatc aaaacatggt
      841 ccaatggttt tagttaatga tggtagcaat aaaagcattt taaaaaatgc taagaagata
      901 acttctatag gatatataga ttcaaatata gtacaacagt gtttaaatat aacaaatgga
      961 attggagata ttaatactgg ggttgtaaaa aatgttaaac ctactacaaa gactataaaa
     1021 gatggaatgt ataaggttgg aaaagatatc tcagcaggtg aatacttaat aacttcaaac
     1081 tcaggaagtt atgcttctta ttatgaagtt acaagtgata gtacaggtaa tgctgatagt
     1141 atactttcaa atgatatttt tagtgggact agatatatca ccttaaagaa tggtcaatat
     1201 attaaaattg aggattcaac aatgacatta gcaaagtatg ctaaagccca gaaggccaag
     1261 aatggtaagt ttggaaatgg tatgtataaa attggattag aaattcctgc tggagaatat
     1321 attataatgt caaattcttc tgatgcatat tatgaagtta gaaatgatag cttaggtaat
     1381 gcagagggaa tagttactaa tgatactttt tcaggaagaa gatatataac ggttgaggaa
     1441 ggtcaatatt taatattaaa tgattgttat ttaattgaaa atgaatagat gtaattgtat
     1501 ttaatagtaa atcatatatt ttatttaagg aaaggtgttt taagttttaa tttaaaac
//
LOCUS       DAMJYR010000274         1528 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.279, whole genome shotgun sequence.
ACCESSION   DAMJYR010000274 DAMJYR010000000
VERSION     DAMJYR010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1528
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.279"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(96..1388)
                     /gene="brnQ"
                     /locus_tag="PC471_003870"
     CDS             complement(96..1388)
                     /gene="brnQ"
                     /locus_tag="PC471_003870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HDF2790927.1"
                     /translation="MGKTKDVIVFGFALFAMFFGAGNLIFPPYLGIITGPEWLIAFLG
                     FTFADAGLALLAVMATAKFDGNVVEMFKRCGMKLGILIGCADILCIGPFLAIPRTGAT
                     TYEMGIMPLFGTSIPVLLFCILFFAISYVLTIRPSKVVDIVGQFLTPALLIALAFIII
                     KGIISPLGDIVDKPMIPNVFAEGIGQGYQTMDAFAAIALASVLIVSLNDKGYSTISDK
                     LKMIGKAGVLACGGLALVYGGLCFLGATVSTMYGTDAVQSQVIVNITEGLLGNVGKAI
                     LAVVVSLACLTTSIGLTSATGQYFSRLTKGKLSYEKIVLAVSVFSAVVASFGVGTIIK
                     IASPILSIVYPPSIVLIILAFFNEKIKNDNVYKGAVYMSLFVSILTVISSYGVAVPVV
                     NSLPLNSLGFNWVVPVIIAGIIGNFIPSKSQSNTLGTN"
BASE COUNT          551 a          280 c          190 g          507 t
ORIGIN      
        1 atctaaaaat aaaaggagtt atttgtacaa ataactcctt ttacttttag ttaactcatt
       61 tatttacaat tgattatatt ttaattaaac aaaacttagt ttgttccaag tgtattagat
      121 tgactctttg atggtataaa attaccaatt attccagcta ttataactgg caccacccag
      181 ttaaatccta atgaattaag aggtaaagaa tttactactg gaacagctac tccataacta
      241 gatataactg ttagtatact tacaaacaat gacatatata ctgctccttt gtatacatta
      301 tcattcttaa ttttttcatt gaagaaagct aaaattatta aaacaattga tggtggataa
      361 actatactta gtataggaga tgctatcttt attatagttc caacaccaaa agatgctact
      421 actgcactga atacagatac agctaatact attttttcat aactcaattt accttttgta
      481 agtctagaga aatattgacc tgtggcagat gtaagaccta tagatgtagt taaacatgct
      541 aatgatacta ctactgctaa aatcgctttc ccaacatttc caagcaatcc ttcagttata
      601 tttactatta cttgtgattg tactgcatct gttccataca ttgttgatac agttgcccca
      661 aggaaacata atcctccata aactagagct aatcctccac aagcaagaac acccgcctta
      721 ccaatcattt tcaacttgtc actaatagta gaatatcctt tatcatttaa agatactatt
      781 agcactgatg caagtgctat tgctgcaaaa gcatccattg tctgatatcc ttgacctata
      841 ccttctgcaa atacattagg tatcataggt ttatctacta tatctccaag aggtgatata
      901 atacctttta taattataaa tgctaaagct attagaagag ctggagttaa aaactgtcca
      961 actatatcta cgaccttaga aggtcttatt gttagtacat atgaaatcgc aaaaaaaagt
     1021 atacagaata ataacactgg aattgaagtt ccaaacaaag gcattatacc catttcataa
     1081 gttgttgcac ctgttcttgg tatagctaag aatggtccta tacataaaat atctgcacaa
     1141 cctataagaa ttcctaactt cataccacat cttttaaaca tttcaactac gtttccatcg
     1201 aatttagctg tagccattac tgctagaagt gccaatccag catcagcaaa cgtaaatcct
     1261 aagaaggcaa ttaaccattc tggacctgta attattccca agtaaggtgg aaatattaag
     1321 ttacctgctc caaagaacat tgcaaataac gcaaatccaa atactataac atccttggtc
     1381 ttacccatta taaaatcccc ctttttaacc ttattaaatt aataaaatgt ctcgattgtt
     1441 tacgcttgca taagtaagtt gaattaaaca ataagaactt attattaaat taataaaatt
     1501 aaataaatta aatttttaat atattgta
//
LOCUS       DAMJYR010000275         1523 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.280, whole genome shotgun sequence.
ACCESSION   DAMJYR010000275 DAMJYR010000000
VERSION     DAMJYR010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1523)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1523)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1523
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.280"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     misc_binding    465..728
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            879..1430
                     /locus_tag="PC471_003871"
     CDS             879..1430
                     /locus_tag="PC471_003871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan transporter"
                     /protein_id="HDF2790928.1"
                     /translation="MKTNTKKLTLNAILLAMGLLIHQITPAIGLPMKPDVPLAMLFVI
                     LVLNRDDYKTCLIAGIVTGIFTALTSSFPGGQIPNVIDKTLTANIVFLLMNISYNMPF
                     IKKLAKKTQDTVVVAIIMPIGTLVSGTIFLLAAQAIVGLPGASFTALFLAVVLPAVLI
                     NLVAGIFLFKIVSLSIRRVSYQA"
BASE COUNT          623 a          150 c          236 g          514 t
ORIGIN      
        1 ttaaaagaaa gaagaaaaaa ttttactaaa aataattaaa agaatatcat ttttttaatc
       61 attggtatct atttaataat atgatgtaat taaaatggag taatattctg tttcaaagga
      121 ggcatataaa tacaggaatg agtattaaat gatggttaat taagtaaatt tattgataaa
      181 tgtacttaat taactttgaa gaatttaatt cctcatgaat caaaatataa aattacatca
      241 acagttattc acaaaattaa tataagaaga attagagcca aagagcagac tattagacca
      301 ttaaagtaat tattcataga attttgtata tgaaaagaaa tgtatagtat aatttacttt
      361 ttttataaaa aatagattac aaaatatatt ttttataaaa aggtattgac attttttaga
      421 aaaaagaata tcattatttt aacaacaaaa ttcaatacaa taaattaaat cctatgacaa
      481 ggaagagtag ttaaatttag gttcagtata gagagctgag gatggtggaa tcttagcaag
      541 aaaaaatttg atgaatggcc cttagaggaa taaattgaaa tcatagtttt gagagtagac
      601 tttatcgtca gtcgcacgtt atagcgaata aggtatgtaa gtacctgata atgagatgta
      661 tgaaaattga attttatatt aatttttata taatttaggt ggcaacgcgg ataatctccg
      721 tcctattagt ttaggacgga gttttttatt ttactgaaaa tttaaaagat agaaggtggt
      781 gagaaagatg ttatgaattt caaaagctaa agatgatgat atagtaggtt ggcaaaaaaa
      841 taaattgaaa attaaaaaaa taggaggaag tacaaaaaat gaaaacaaat acaaaaaaat
      901 taactttaaa tgcaatactt ttagcgatgg gattattaat acatcaaatc acaccagcta
      961 taggattacc aatgaagcca gatgtaccac tagcaatgtt atttgtaata ttggttttaa
     1021 atagagatga ttataaaact tgtttgatag cgggtatagt aacaggaata tttacagcac
     1081 ttacatcaag ttttccggga ggtcaaattc ctaatgtaat agataaaact cttacagcaa
     1141 atatagtatt tttattaatg aatattagtt ataatatgcc ttttataaaa aaattagcta
     1201 agaaaacaca agatactgta gtagttgcaa taataatgcc aataggaact cttgtaagtg
     1261 gaactatctt tttattagca gctcaagcta tagttggtct tccaggggct tcatttacag
     1321 ctttattctt agcagtagta ttaccagctg ttttaataaa tctagttgca ggaatatttt
     1381 tattcaagat tgttagtcta tcaataagaa gagttagtta tcaagcttaa acacgaaata
     1441 atattttact ttataaagca ttataataac taaataacat aagaataaag tagattataa
     1501 ctattttatt cttatgttgt tta
//
LOCUS       DAMJYR010000276         1482 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.281, whole genome shotgun sequence.
ACCESSION   DAMJYR010000276 DAMJYR010000000
VERSION     DAMJYR010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1482)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1482)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1482
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.281"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            18..1298
                     /locus_tag="PC471_003872"
     CDS             18..1298
                     /locus_tag="PC471_003872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HDF2790929.1"
                     /translation="MNILGLIVEYNPFHNGHIYHLSKSIEKTNATHTVAIMSGNFLQR
                     GEPALFDKYTRAKIAVENGVDLVIELPTLFACQSAEIFSHGAITILNSLNCINSICFG
                     SEEGNTEILYNLAEILANEPNEFKLLLKKYLDDGLLFPTARANALFDYIHQDKKNNFI
                     QNYSKEQLSKTLNSSNNILGIEYIKNLIHLNSSIKPFTISRVQSEYNSEEIESDICSA
                     TAIRTSLKENTDLFHLKNVIPEKTYNILNEKIDNGFLPMFDDFFFDILKTIILRDMDT
                     LKEYFEVNEGIENRIYKDIFTSEFLHELQLAIKSKRYTLTKIKRTLNNILLGIKKSDV
                     QLIKNIDTIPYIRILAFNDKGREILKEIKNKSEIKIINKFSNISFSLDDTIFKTLISY
                     DIKATNIYNSIYYKNNKDLVKGPMDFYTHPIYVK"
BASE COUNT          621 a          194 c          171 g          496 t
ORIGIN      
        1 gataaaagga gtattttatg aacatacttg gattaatagt tgaatataac ccatttcata
       61 atggacacat atatcattta tctaaatcta tagaaaaaac aaatgcaaca catactgtag
      121 caataatgag tggaaatttt ctacaaagag gcgaaccagc tttatttgat aaatacacta
      181 gagcaaaaat tgctgtcgaa aatggtgtag acttagttat agaattacca actttatttg
      241 cttgtcaaag tgctgaaatt ttttctcatg gtgctataac aattttaaat tcactaaact
      301 gcataaattc tatatgtttt ggtagtgaag aaggaaatac agaaattctt tataatctag
      361 ctgaaatttt agcaaatgaa ccaaatgaat ttaagttact tttaaaaaaa tatttagatg
      421 atggtctgtt atttccaaca gctagagcta atgcactttt tgattatata catcaagaca
      481 aaaaaaataa ttttatacaa aattattcaa aggaacaatt atcaaaaaca ttaaattcat
      541 caaacaacat actgggtata gaatacataa aaaacctcat ccatttaaac agtagtataa
      601 aaccatttac tatctccagg gtccaatcag aatataattc cgaagaaatt gagagtgata
      661 tttgttctgc tactgctata agaacttcac ttaaagaaaa tacagacctt tttcatttaa
      721 aaaatgttat tccagaaaaa acttataaca tcctaaatga aaaaatagat aatggttttt
      781 tacctatgtt tgatgacttc ttctttgata ttttaaaaac aattatactt agagatatgg
      841 acactttaaa agagtatttt gaagtaaatg aaggcataga aaacagaatc tataaagata
      901 tctttacatc tgaatttcta catgaactac aattagcaat taaatccaag cgatacactt
      961 taaccaaaat caaacgaact ctaaacaata tattgttagg tattaaaaaa agcgacgtgc
     1021 aacttataaa aaacatagat actatacctt atataagaat acttgctttt aatgataaag
     1081 gtagagaaat tctaaaagag ataaaaaata aatctgaaat aaaaataata aacaagtttt
     1141 caaatatatc attttcatta gatgatacga tttttaaaac actcataagt tatgatataa
     1201 aagctacaaa tatatataat tcaatatatt ataaaaacaa taaagattta gtaaaaggcc
     1261 ctatggattt ttatactcat ccaatatatg taaagtagtt ttttaaaata actatattta
     1321 taaaaatcat tttattaact ctttattata taagatacaa tagtcttact ttatttatgt
     1381 acacatgctt ttaggatgac aacatcaact taaatattat gttactttct aaatttaagt
     1441 aaatattgcc atcctaaaaa ttttacaatt tagcttcaga tt
//
LOCUS       DAMJYR010000277         1429 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.282, whole genome shotgun sequence.
ACCESSION   DAMJYR010000277 DAMJYR010000000
VERSION     DAMJYR010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1429
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.282"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            18..629
                     /locus_tag="PC471_003873"
     CDS             18..629
                     /locus_tag="PC471_003873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HDF2790930.1"
                     /translation="MINDIKNKFKNYKPYINGYKNMKRASVLIPIVEINNTHYILFEV
                     RSKNLKHQPSEISFPGGKIESGETPYEAVIRETCEELGTFSDNIEVISSLDLLITPVK
                     FIIHPYLGYIKNINNLNINKDEVDHTFLVPIKYLLENPPNTYSNTVKVFPDENLPYGS
                     IPNQKDYKFESGSYPILFYKYKDYVIWGMTAKILENFLNFLSE"
     gene            927..1265
                     /locus_tag="PC471_003874"
     CDS             927..1265
                     /locus_tag="PC471_003874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3784 domain-containing protein"
                     /protein_id="HDF2790931.1"
                     /translation="MFAVGFVVIIALAFFILSVLLFNGKASMLLAGYNSMSKKEKARY
                     DKKKLCRSSSIVTFIVSIMLFIMAFLGYRIESGQMDEKKMVPFSIIFIIVILSSVFFN
                     ILYSNKKCKK"
BASE COUNT          561 a          161 c          189 g          518 t
ORIGIN      
        1 tattttagga ggtaaaaatg ataaacgata ttaaaaataa attcaaaaat tacaaaccat
       61 atataaatgg atacaagaat atgaagagag catctgtttt aattccaata gtagaaataa
      121 ataacacaca ctatatatta tttgaagttc gctcaaaaaa tttgaagcat caacctagcg
      181 aaatttcttt tccaggtggt aaaatagaat caggtgagac tccatatgaa gctgttatta
      241 gagaaacttg tgaggaactt ggaactttta gtgacaatat agaagtaata tcaagtcttg
      301 acctccttat aacaccagta aagtttatca tacatccata cttaggatat ataaaaaata
      361 ttaataattt aaatataaac aaggatgaag ttgaccatac ttttttggtt cctataaaat
      421 atcttttaga gaatcctcca aatacttata gtaatactgt aaaagttttt ccagatgaaa
      481 atctaccata tggttcaatt ccaaaccaaa aagattataa atttgaaagt ggaagttatc
      541 ctattctgtt ttataaatat aaagattatg taatttgggg aatgacagca aaaatactag
      601 aaaacttttt aaatttttta tcagaataac actcataata aattatcaaa atttttacat
      661 aagaaattta taaaagtttc taaatttaac aattctaaaa tatcaataac tatattttgt
      721 agcacttttt tattttatta ttgtttgaat atttgatggt tttcaatgca atttcaacat
      781 aaatactata tattagtgta tactgttgat gaattaaagt atgataatct atttaataaa
      841 gtatgaagta aatgatttta atgtgccttt atggtaaaat tactaataag taataaaatt
      901 aattaaaata tatggaggtc cttataatgt ttgctgtagg atttgttgtt ataattgcac
      961 ttgccttttt cattttaagt gtactattat ttaatggaaa agccagtatg ctactagcag
     1021 gttataactc tatgagtaag aaagaaaaag cacgatacga taaaaaaaaa ttatgtagaa
     1081 gttctagcat tgttactttt attgtctcta taatgctatt tattatggcg tttttgggat
     1141 atagaataga gagtggtcaa atggacgaaa aaaaaatggt tcccttttct ataatattta
     1201 taatagtcat actttccagt gtatttttta atatcctcta ctctaacaaa aaatgtaaaa
     1261 aataaatagt tgtaagttga gcagttaatt ataaaagatt gactgctttt tcatgtatat
     1321 tgactactct tattaaatat tttaacattt aaaaatctat gtaaaaataa tgaatatagt
     1381 acaaacttct aataatgatt ttacctttgt ggttacagtc cactatata
//
LOCUS       DAMJYR010000278         1400 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.283, whole genome shotgun sequence.
ACCESSION   DAMJYR010000278 DAMJYR010000000
VERSION     DAMJYR010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1400
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.283"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            <1..957
                     /locus_tag="PC471_003875"
     CDS             <1..957
                     /locus_tag="PC471_003875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2790932.1"
                     /translation="IKYKNEYSKKISVEKDNVYMLKNNGKVTHRKVNGESSSIPGWEN
                     IISIASYDNSIIGIKKDGTVVSKGLGNSDSDNISKWRDIIDIECDSGTFIGLKKDGTV
                     VATGNNDYGQCNVNNWFDVVSIEITKSTIEYMKNTTVIGIKKDGKVLLTGYDSLNHRK
                     ETDKWRDISKIKCGSYGIFGIKNNGTVVVSGSAYDIKNWKDIVDIHIGLCGIVGIKRD
                     GTIISTDEKFNEIIKNWKDIISAEIIDNHEGISIVGINSNGDILTSSRGQNLDNIKRL
                     EDVEYINFDDSIDNFLVGIRKDNVIFIRGINNTINEEYNEEI"
     gene            991..1347
                     /locus_tag="PC471_003876"
     CDS             991..1347
                     /locus_tag="PC471_003876"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2790933.1"
                     /translation="MKNYIARCLSNMGVLLAGIQVLAGIIIMCQKVEVPYDYLSDTYE
                     QYSPIMIGIGAGVIVSGIVICLVLKGLAELVENSADQTQYQKEILMLLKNNNVNEKIN
                     EVKIEQQFTNEELPKL"
BASE COUNT          609 a           98 c          266 g          427 t
ORIGIN      
        1 ataaagtata aaaatgaata tagtaaaaaa atatctgtag aaaaagataa tgtatatatg
       61 ttaaaaaata atggaaaagt aacacataga aaagtgaatg gtgaaagtag ttctatacct
      121 ggttgggaaa atataatatc tatagcatca tatgataact caataatagg aataaaaaaa
      181 gatggtacag ttgtttctaa aggacttgga aattcagatt cagacaatat tagtaaatgg
      241 agagatataa tagatataga atgtgatagt ggaactttta taggattaaa aaaggatggt
      301 actgtcgttg ctactggaaa taatgattat ggtcaatgca atgtgaataa ttggtttgat
      361 gtagtaagta tagaaataac aaaatcaacc attgagtata tgaaaaatac aacagtaata
      421 ggcataaaaa aagatggaaa agtattactt acaggatatg atagtctgaa tcatagaaaa
      481 gaaacagaca aatggagaga tatatcaaag ataaaatgtg ggtcttatgg catatttgga
      541 ataaagaata atggcacagt agttgtatca ggtagtgctt atgatattaa gaattggaaa
      601 gatatagtgg atatacatat tggattatgt ggaatagtgg gtattaaaag agatgggaca
      661 ataataagca cagatgaaaa gtttaatgaa atcattaaaa attggaagga tattatttct
      721 gctgaaataa tagataatca tgaaggaatt tctattgtag gaattaattc aaatggagat
      781 attttaacat ctagtagagg tcagaatttg gataatataa aaagattaga agatgtagaa
      841 tatataaatt ttgatgactc gattgataat tttttagtag gtataagaaa agataatgta
      901 atttttataa gaggaataaa caatactata aatgaagaat acaacgaaga aatataaaaa
      961 taaatatttt aatttggagg aatagaaaat atgaaaaatt acatagcaag atgtttatca
     1021 aatatgggag tattgttggc aggtatacaa gtattagcgg gtataataat catgtgtcaa
     1081 aaagtagaag ttccatatga ttatttgtca gatacttatg aacaatatag tcctattatg
     1141 attggtattg gtgctggggt tatagtaagt ggaatcgtta tatgtttagt tttaaaggga
     1201 ttagcagagt tagttgaaaa ttcagctgac caaacacaat atcaaaaaga aatattaatg
     1261 ttgttgaaaa ataataatgt aaatgaaaaa attaatgagg ttaaaattga acaacaattt
     1321 acaaatgagg aattaccaaa attataaaat gttttaaatt atatactaat actcagtact
     1381 aattaaagta ctgagtattt
//
LOCUS       DAMJYR010000279         1378 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.284, whole genome shotgun sequence.
ACCESSION   DAMJYR010000279 DAMJYR010000000
VERSION     DAMJYR010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1378)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1378)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1378
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.284"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(<1..161)
                     /locus_tag="PC471_003877"
     CDS             complement(<1..161)
                     /locus_tag="PC471_003877"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDF2790934.1"
                     /translation="MLNINVFWIFLPIFSTAIEWIVLKFLLNEFSELRKNRLTLNSSI
                     VIAIMIITM"
     gene            complement(155..283)
                     /locus_tag="PC471_003878"
     CDS             complement(155..283)
                     /locus_tag="PC471_003878"
                     /inference="COORDINATES: protein motif:HMM:NF016296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2790935.1"
                     /translation="MEYIQFICKNTVVINGKDIPVSKYRMANLKKKLTYVLGDVLC"
     gene            complement(422..1132)
                     /locus_tag="PC471_003879"
     CDS             complement(422..1132)
                     /locus_tag="PC471_003879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2790936.1"
                     /translation="MYRIVICEDDITQIAFLRECILKSLEGISSQIELFEFNSGEELL
                     ETNLEGIDIFFLDIKMLQLTGMDVAKIIRETNDTSEIIFITSIVDYIQEGYKVRAYRY
                     LLKPIDFGDLNESILSCISDIIKKRENFMLIENKGIINKILINSIMYIEVRKKVLTIH
                     TKNDTYYTKNSMDKIELELEKYNFFRCHKSYLINLEYIQFICKKTVVINDEDVPVSKY
                     RMADLKKKLTYVLGDVLC"
BASE COUNT          525 a          220 c           87 g          546 t
ORIGIN      
        1 aacatagtaa ttatcataat agcaattact atgctactat ttagggttaa tctattcttt
       61 cttaactcac taaattcatt taataaaaat tttaatacaa tccattctat cgccgtagaa
      121 aatataggta aaaaaatcca aaatacattt atatttaaca taatacatct cccaatacat
      181 atgttaattt tttctttaaa ttagccattc tatatttact aactggtata tctttaccat
      241 taataactac agtattttta catataaatt gaatatactc caaattaatt aaatagctct
      301 tgtaacattt ttaaaaattt attctttctt aaatcactga attcatttga taaaaatttt
      361 aatacaattc attctatcgc cgtagaaaat ataggtaaaa aaatccaaaa tacatttata
      421 tctaacataa tacatctccc aatacatatg ttaatttttt ctttaaatca gccattctat
      481 atttactaac tggtacatct tcatcattaa taaccacagt ctttttacat ataaattgaa
      541 tatactccaa attaattaaa tagctcttat gacatctaaa aaaattatat ttttctagct
      601 ctaattctat tttatccata ctatttttag tataataagt atcattttta gtatgtatag
      661 tcaaaacctt tttcctaacc tctatataca taatactatt aattaaaatt ttattgatta
      721 tacctttatt ttctatcagc ataaaatttt ctcttttttt tataatatcc gatatacaac
      781 tcaaaatact ttcatttaaa tctccaaaat ctataggctt taataaatat ctataagccc
      841 ttaccttata gccttcctgt atgtaatcaa ctattgaagt tataaatatt atttcagaag
      901 tatcattagt ttctctaatt attttagcca catccatgcc tgttagttga agcattttaa
      961 tatctaaaaa aaatatatca ataccttcta aatttgtttc caataattct tctcctgaat
     1021 taaactcaaa tagttcaatt tgactagaaa taccctctaa ggactttaat atacactctc
     1081 ttaaaaaagc tatttgtgta atatcatcct cacagataac tattctatac ataaacaccc
     1141 ctccttagac attagatctg aaaacaaata aaatatcaat ttataaataa ttatttagtt
     1201 atacaactaa catggtcatc aaatactatt attcacttaa aaaatcaccc cctattttta
     1261 gtatgttaac aacaaaataa gaagatatct tagattttta aaaacaatta atacttaatg
     1321 tagttttgta tactatttat aggtaatttt ttatatcatt ttaaacatta aaattatt
//
LOCUS       DAMJYR010000280         1352 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.285, whole genome shotgun sequence.
ACCESSION   DAMJYR010000280 DAMJYR010000000
VERSION     DAMJYR010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1352
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.285"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            303..1013
                     /gene="rgaR"
                     /locus_tag="PC471_003880"
     CDS             303..1013
                     /gene="rgaR"
                     /locus_tag="PC471_003880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RgaR"
                     /protein_id="HDF2790937.1"
                     /translation="MFRVVICDDEKIQRSILKEFTEKLLSERCLNYQILEFSCGEDLI
                     SKYPEKIDIIFLDIQMKDINGIEVAKKIRKFDEKVEIIFTTAFSEYAPQGYEVRAYRY
                     LVKPIEYSNFAMGVNLCIDNLQRKKERYIVLNSKKGFNRILIDSILYVETVKRDLVVH
                     TIGRDYKANMSMKQAEKLLCENGFFRCHTSFIVNLRRIEDLRDNMITINEQFIPISKY
                     RLKDLKVALTSLLCDIMY"
BASE COUNT          536 a          106 c          210 g          500 t
ORIGIN      
        1 ttgtgaatat atgaatttta tgtcttaaaa tttataagca ataaaaattt gatatattat
       61 gatgtttaat atagggtaat aaatacatat tatatttatt ttactatatt tttttacata
      121 atatatctag aaaagtatag gaatttatcg taatttaagt ttaaatatta aagagaattt
      181 ctataagtag tttaatcgac aatttaaatt tataaagaaa tttaaatttg aaagctagtg
      241 agtacagttt ggaaattgaa ataaagtaaa ctaaatgata ttttagattt ggaggtgata
      301 ttatgttcag ggtagttata tgtgatgacg aaaaaataca aagaagcata ctaaaagagt
      361 ttacagaaaa acttttgtct gaaagatgtc taaattatca aatactggaa ttttcctgtg
      421 gagaagattt aatatccaaa tacccagaaa aaattgatat tatattttta gatatccaaa
      481 tgaaagatat caatggaatt gaggtggcta aaaaaataag aaagtttgat gaaaaagtag
      541 aaattatatt tacaacggct ttttcagagt atgcccccca aggttatgag gttcgtgctt
      601 atagatattt agtaaagcct atagagtata gtaactttgc aatgggtgta aatctttgta
      661 ttgataacct tcaaagaaag aaggagcgtt atatagtttt aaatagtaaa aaaggattta
      721 atagaatact gatagattct attttatatg ttgaaactgt aaagagagat ttagtcgtac
      781 atacaatagg tagagattat aaagctaata tgagcatgaa acaagcagaa aaattactgt
      841 gtgaaaatgg cttttttaga tgtcatacaa gttttattgt taatttaaga agaatagaag
      901 atcttaggga taatatgatt actattaatg aacaatttat acctataagt aagtatagac
      961 ttaaagattt aaaagtagca ttaacaagct tgttatgtga tataatgtat taattttaaa
     1021 aaggtaattg aaaatatcgt aaaaaagatt taatgtcatc acagggctat tgattgaaac
     1081 tgtcttaata tataaaatgt tatttatatt aagacagttt tattatgcaa aatagcagtt
     1141 tatagatttt taatatggtt taacatgtat atgtagaggt tccattatag tagcatgatt
     1201 attaattagt agcttgatta tcaatatact attattgatt tttatatgca ttatatgtat
     1261 ttttagaata tgattacctt tttaaaataa tgattattta taaaactcta tagatatttg
     1321 tttcatttag ctacgcatac tttaattaat ta
//
LOCUS       DAMJYR010000281         1348 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.286, whole genome shotgun sequence.
ACCESSION   DAMJYR010000281 DAMJYR010000000
VERSION     DAMJYR010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1348)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1348)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1348
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.286"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            201..1298
                     /gene="ychF"
                     /locus_tag="PC471_003881"
     CDS             201..1298
                     /gene="ychF"
                     /locus_tag="PC471_003881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HDF2790938.1"
                     /translation="MKLGIVGLPNVGKSTLFNAITQAGAESANYPFCTIDPNVGVVSV
                     PDERLNKLQELYNSEKIVPTAIEFCDIAGLVRGASKGEGLGNKFLSHIREVDAIVHVV
                     RCFEDENVVHVDGSVDPLRDIETINLELIFSDIEILERRINKTQKAAKADKTLGSELD
                     LLKSIMSTLEESKCVRTMEFTEDEQTFVNSLDLLTSKPVIYASNVSEDDLADNGENNK
                     YVQQVKAFAETEDAEVVVVCAQIEAEISELDSAEEKKEFLETLGLEQSGLDKLIKSSY
                     ALLGLISFLTAGPKEVRAWTIKVGSKAPQAGGKIHSDIERGFIRAETIAFNDLVEHGT
                     MAAAKEKGLVKLEGKEYIVKDGDVILFRFNV"
BASE COUNT          527 a          148 c          241 g          432 t
ORIGIN      
        1 tctaaaagca atatttttat ttaaattaaa tacaaatatt atttaaatca atatgcttta
       61 aagtctagtt tacaagcttg atatatatga tgtataatca atatgtatgt ctataattaa
      121 aacaattttg caatcctata gattgattgt ttaaagttat ataaagaata taaaaaagaa
      181 taagagagag gacgattaat atgaaattag gtatagttgg tttaccaaat gtaggtaaaa
      241 gcacactatt taacgcaata actcaagcag gtgcagaatc tgcaaattac cctttttgta
      301 caatagaccc aaatgtggga gttgtatctg ttccagatga aagattaaac aaactacaag
      361 aattatataa ttctgaaaaa atagttccta ctgctataga attttgtgat atagcaggtt
      421 tagttagagg agcttctaaa ggagaaggtt taggaaataa attcttatca catataagag
      481 aagttgatgc aatagttcat gttgttagat gttttgagga tgaaaatgtt gtacatgtag
      541 atggttctgt agacccactt cgtgatatag aaactataaa cttagaatta attttctcag
      601 atatagagat tcttgaaaga agaattaata aaactcaaaa agctgctaag gctgataaaa
      661 ctttaggttc tgaacttgat ttattaaaat caataatgtc tactttagaa gaaagtaaat
      721 gtgtaagaac tatggaattt acagaagatg aacaaacatt cgtaaattca ttggatttac
      781 ttacatctaa gcctgtaata tatgcttcaa atgtatcaga agatgattta gctgataatg
      841 gagaaaataa taaatatgtt caacaagtta aagcatttgc agaaacagaa gatgccgaag
      901 ttgttgtagt atgtgcacaa atagaagcag aaatttctga acttgattct gctgaagaaa
      961 agaaagagtt tttagaaaca ttaggtcttg aacaatcagg gcttgataag cttataaaat
     1021 cttcttatgc tttacttggt ttaatttcat tcttaactgc tggtccaaaa gaagttagag
     1081 catggactat aaaagttgga agtaaagctc ctcaggcagg aggaaaaata cattctgata
     1141 tagaaagagg atttatcaga gcagaaacaa tagcctttaa tgatttggtt gagcatggta
     1201 ctatggctgc tgctaaagaa aaagggttgg ttaaattaga aggtaaagaa tacatagtta
     1261 aagatggaga tgtaatatta tttagattta atgtttaaaa tgattaatcg tatacacttt
     1321 gactattata tataataact aaaaaact
//
LOCUS       DAMJYR010000282         1337 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.287, whole genome shotgun sequence.
ACCESSION   DAMJYR010000282 DAMJYR010000000
VERSION     DAMJYR010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1337
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.287"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            27..101
                     /locus_tag="PC471_003882"
     tRNA            27..101
                     /locus_tag="PC471_003882"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:59..61,aa:Thr,seq:tgt)
     gene            116..200
                     /locus_tag="PC471_003883"
     tRNA            116..200
                     /locus_tag="PC471_003883"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:150..152,aa:Tyr,seq:gta)
     gene            210..293
                     /locus_tag="PC471_003884"
     tRNA            210..293
                     /locus_tag="PC471_003884"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:244..246,aa:Leu,seq:tag)
     gene            323..399
                     /locus_tag="PC471_003885"
     tRNA            323..399
                     /locus_tag="PC471_003885"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:357..359,aa:Arg,seq:tct)
     gene            407..482
                     /locus_tag="PC471_003886"
     tRNA            407..482
                     /locus_tag="PC471_003886"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:440..442,aa:Gln,seq:ttg)
     gene            571..659
                     /locus_tag="PC471_003887"
     tRNA            571..659
                     /locus_tag="PC471_003887"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:605..607,aa:Ser,seq:tga)
     gene            663..738
                     /locus_tag="PC471_003888"
     tRNA            663..738
                     /locus_tag="PC471_003888"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:696..698,aa:Phe,seq:gaa)
     gene            745..818
                     /locus_tag="PC471_003889"
     tRNA            745..818
                     /locus_tag="PC471_003889"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:779..781,aa:Met,seq:cat)
     gene            833..909
                     /locus_tag="PC471_003890"
     tRNA            833..909
                     /locus_tag="PC471_003890"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:867..869,aa:Ile,seq:cat)
     gene            933..1009
                     /locus_tag="PC471_003891"
     tRNA            933..1009
                     /locus_tag="PC471_003891"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:967..969,aa:Pro,seq:tgg)
     gene            1017..1093
                     /locus_tag="PC471_003892"
     tRNA            1017..1093
                     /locus_tag="PC471_003892"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1051..1053,aa:His,seq:gtg)
     gene            1102..1177
                     /locus_tag="PC471_003893"
     tRNA            1102..1177
                     /locus_tag="PC471_003893"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1135..1137,aa:Lys,seq:ttt)
     gene            1185..1258
                     /locus_tag="PC471_003894"
     tRNA            1185..1258
                     /locus_tag="PC471_003894"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1217..1219,aa:Cys,seq:gca)
     gene            1265..1336
                     /locus_tag="PC471_003895"
     tRNA            1265..1336
                     /locus_tag="PC471_003895"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1297..1299,aa:Asn,seq:gtt)
BASE COUNT          321 a          309 c          355 g          352 t
ORIGIN      
        1 ccccttccag gtcgccaaaa taaaatgctg gtgtggctca acggtagagc agctgacttg
       61 taatcagcag gttgtaggtt cgattcctat caccagctcc atgtaaaatt aatacggagg
      121 atttcccgag cggccaaagg gggcagactg taaatctgtt gtcatcgact tcggtggttc
      181 gaatccacca tcctccacca aaaaaatgcg cggatgtggc ggaattggca gacgcactag
      241 acttaggatc tagcgccatt ggcgtggggg ttcgactccc ttcatccgca ccatttttgt
      301 ttgatataca tatataaatt gtgggtgcat agctcagctg gatagagcaa cgcccttcta
      361 aggcgtgtgt ccggggttcg aatccctgtg cgctcaccac atatattggg gattagccaa
      421 gtcggtaagg cacatgactt tgactcatgt atgcgtaggt tcgagtcctg catccccagc
      481 caaatgatgt attactaagc tatatttgaa ttatttttat tttaaatata gttaggttca
      541 attttagaat aagtttaagt atccgaatgt ggagaggtgt ccgagtggtt taaggagctg
      601 gtcttgaaaa ccagtgactt cgaaaggggc cgtgggttcg aatcccaccc tctccgccaa
      661 atgcccagat agctcagtcg gtagagcagg ggactgaaaa tccccgtgtc ggtggttcga
      721 ttccgcctct gggcaccaaa atatggcggt atagcttagt tggctagagc gttcggttca
      781 tacccgaaag gtcataggtt cgactcctat taccgctact aaaatttaat gtgggccatt
      841 agctcagttg gttagagcgc ccggctcata accggtaggt ctggggttcg agtccctgat
      901 ggcccaccac attaaattaa ttaatataaa ttcgaggtgt agcgcagttt ggtagcgcac
      961 atggtttggg accatggggc cgggggttcg agtcccttca cctcgaccaa tataaggtgg
     1021 gtatagctca gttggttaga gcgccagatt gtggctctgg aggtcgtgag ttcgactctc
     1081 attatccacc ccaattcata tggtctatta gctcagtcgg tagagcacct gacttttaat
     1141 cagggtgtcc cgcgttcgag tcgcggatag atcaccaaaa aggtggcgac atagccaagt
     1201 ggtaaggcag tggactgcaa ctccttgatc cccagttcga atctgggtgt cgcctccaaa
     1261 atattccaag gtagctcaac ggtggagcaa ccggctgtta accggtaggc tgtgggttcg
     1321 agccccaccc ttggagc
//
LOCUS       DAMJYR010000283         1336 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.288, whole genome shotgun sequence.
ACCESSION   DAMJYR010000283 DAMJYR010000000
VERSION     DAMJYR010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1336)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1336)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1336
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.288"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            163..858
                     /gene="cas6"
                     /locus_tag="PC471_003896"
     CDS             163..858
                     /gene="cas6"
                     /locus_tag="PC471_003896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endoribonuclease Cas6"
                     /protein_id="HDF2790939.1"
                     /translation="MIKCLEMSVKVYLLENISLDNTSKEIRKLIDDTLIIEEKYKEFH
                     NKAGFKNYSFNLLYPLEKDKVYKKDCIYTFIIRTVDSELGMYFMNNLKNQHSDKIKVI
                     TMSKKVIPKKHIESIYSVTPIIAKFEDGYWRSNKTVEDLEKRIRENLIKKYNEFTNSK
                     IDEDFELFQVIKFINRKPISTKYKNISLLGDKISFKVSENDMAQEIANFALGVGVGEI
                     NSRGFGFVNYRWL"
     gene            849..>1336
                     /locus_tag="PC471_003897"
     CDS             849..>1336
                     /locus_tag="PC471_003897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated protein Cse4"
                     /protein_id="HDF2790940.1"
                     /translation="MVIGGENLLKDCLEIFEKEYKSLGNRLITDSYVLSYGTYILVKN
                     FSNIDILEVSKNNVDSTEDIYHKFCELDYLSKLVSIDKSVDSKKIIHSNNYLSFFVKK
                     ENLRPDKNNNVKLTEEIIDNYYDALLNPRIKYKSDKRKKEIYDSIENKYGKVDENKLN
                     KI"
BASE COUNT          590 a           96 c          201 g          449 t
ORIGIN      
        1 aaactttgtg attttatttg tcgaataaaa aataaaaaat tttaaaggaa aaagttaaaa
       61 aataaagaat tttataacta taaattatat tttagagttt attgttatta aatagatgaa
      121 aaaatcctaa tatggattat cagaaaatga ggaggttgtt aattgataaa atgtcttgaa
      181 atgtctgtaa aagtgtatct tcttgaaaat atttctttag ataatacatc aaaagaaata
      241 cgtaaattaa tagatgatac attaattata gaagaaaaat ataaggaatt tcataataaa
      301 gctggattta agaattattc ttttaattta ttatacccat tagaaaaaga taaggtttat
      361 aagaaggatt gtatatatac ttttattatt agaactgtag atagtgaatt aggaatgtat
      421 tttatgaata atttaaaaaa tcagcattct gataaaataa aggttattac aatgagtaaa
      481 aaagtgatcc ctaaaaaaca tatagaaagt atttactctg tcactccaat aatagctaaa
      541 tttgaggatg ggtattggag aagtaataaa acagtagaag atttagaaaa aagaatcaga
      601 gaaaatttaa taaaaaaata taatgaattt actaacagta aaatagatga agattttgaa
      661 ttatttcaag ttattaaatt tataaatagg aaacctattt ctactaaata taagaatata
      721 tctttacttg gtgataaaat aagttttaaa gtatcagaaa atgatatggc tcaggaaatt
      781 gcaaattttg cacttggagt tggagtcgga gaaattaatt ctcgtggatt tggttttgta
      841 aactatagat ggttatagga ggtgaaaact tgttaaagga ttgtttagag atttttgaaa
      901 aagaatacaa atctttagga aatagattga taactgatag ctatgtccta tcctatggaa
      961 catatatatt ggtgaaaaat ttttcgaata tagatatatt agaagtttct aaaaataatg
     1021 tggatagtac agaagatata taccataaat tttgtgaact ggattattta tcaaaactgg
     1081 tgtctataga taaatcagtt gattccaaaa aaataataca tagtaataat tatctttcat
     1141 tttttgtaaa aaaggaaaac ttaagacctg ataaaaataa taatgtaaaa cttacagaag
     1201 aaattattga taattattat gatgctttgt taaacccaag aattaaatat aaatctgata
     1261 aaagaaaaaa agaaatctat gattcaatag aaaataaata tggtaaagtg gatgaaaata
     1321 aattaaataa aataag
//
LOCUS       DAMJYR010000284         1289 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.289, whole genome shotgun sequence.
ACCESSION   DAMJYR010000284 DAMJYR010000000
VERSION     DAMJYR010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1289)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1289)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1289
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.289"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            8..271
                     /locus_tag="PC471_003898"
     CDS             8..271
                     /locus_tag="PC471_003898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2790941.1"
                     /translation="MIKNTKQITVEVNEILLQEMSKITNDENNILTDLINESIVNYIL
                     ENKKHNIENKDLEEVIKFYKKENTEKPKSAARAIAEAKTHTLY"
     gene            complement(708..1085)
                     /locus_tag="PC471_003899"
     CDS             complement(708..1085)
                     /locus_tag="PC471_003899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HDF2790942.1"
                     /translation="MTITKIPPAELKVMKFIWSSDFTVASKDVIEAMEKLYNWKQTTT
                     LTLLSRLVNKQFLSARKIDRHTHYTVLIGEEEYLHFETKTFLDNMHGSSIESFMKSLF
                     KKGVNEEELNSVEKWVKDIKKDE"
BASE COUNT          536 a          174 c          106 g          473 t
ORIGIN      
        1 ggttgatatg ataaaaaata caaaacaaat tacagtagaa gttaatgaaa ttttattaca
       61 agaaatgtct aagataacta atgacgaaaa taatattttg acagatttga taaatgaatc
      121 aattgtaaat tatattcttg aaaacaaaaa acataatatt gaaaataaag atttagaaga
      181 agtgattaaa ttttacaaaa aagaaaatac tgaaaaacct aaaagtgcag caagagctat
      241 agctgaagca aaaactcata ctctgtatta atcaaataaa aaagttccta aaaactagga
      301 acttttttat attaataatt attaatatat ctcaatattt atctcaaatc tatcaatata
      361 agcatgttta atacaatcta ttgtatactt atcttaccct agaaataatc aaaacaaaaa
      421 taaaattatg ccctttatat ctaaaacaat taatttacat gattaatata caaaaattct
      481 gacttttaaa ttcgtataca atatcaaact aaaataatat taatgacaaa cccaatataa
      541 attaaattca aacaggtttg attttacatc aaacttattg tattaatatt aaataacaag
      601 acaaaaatat ttataaaaat cacaaatttt acataaaact ttatatttga ttcatttaac
      661 taattattgt aaaaactatt tattttatga taattaacta caccaaatta ttcatctttt
      721 ttaatatcct taacccattt ttcaacagag ttcaattctt cttcatttac cccctttttg
      781 aacaaggatt tcataaaact ctcaatagaa cttccatgca tattatctaa aaaagttttt
      841 gtttcaaaat gtaaatactc ttcttctcca ataagaactg tataatgtgt atgtctatca
      901 atttttcttg cacttaagaa ttgcttattt actaatcttg ataaaagtgt tagtgttgta
      961 gtttgcttcc aattatatag tttttccata gcttcaatga catcttttga tgctactgta
     1021 aaatctgaac tccaaataaa tttcatgact tttaattctg ctggtggtat cttagtaata
     1081 gtcatatgat tttacactcc tctcaaactt ctacataatt aaatactata ttaatagact
     1141 tttcgagtca aaggttttat ttaaaatatc tcatccaaat ttattaatac aaaaaattta
     1201 tatttttatt caaaattaaa cacacaatct attaatataa agataaatat ctattctata
     1261 agtataacag attatcaaat taaaattct
//
LOCUS       DAMJYR010000285         1282 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.290, whole genome shotgun sequence.
ACCESSION   DAMJYR010000285 DAMJYR010000000
VERSION     DAMJYR010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1282
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.290"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(168..434)
                     /locus_tag="PC471_003900"
     CDS             complement(168..434)
                     /locus_tag="PC471_003900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3795 domain-containing protein"
                     /protein_id="HDF2790943.1"
                     /translation="MIESRCGILCYECGYKEKVNCKGCTEIEKPFWGEQCPVKSCCED
                     KNLTHCGLCDTFPCEMLNQFAYDKEQGDDGKRISQCKKWAVLAL"
     gene            complement(476..1231)
                     /locus_tag="PC471_003901"
     CDS             complement(476..1231)
                     /locus_tag="PC471_003901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2790944.1"
                     /translation="MPHKLLRQYIAHYTISIPDTSIKENLTLIPDASGCMIFKFDKKG
                     IESAFWGATTKTTIVKNDIENVLFRVFVEFRPGGVYYLTGLSQRESTDLKISLEDFNT
                     LFSLEVNSIFERTSTIKELVEQLDMLFLSYLLKSNIVDMTIPILENARKQNSIMSVKN
                     ISQISCYSERHLNRIFNNSLGMSVKSYLRLLRINLVLQEIQNNKIPFATLAQDIGYYD
                     QSHFINDFKSICGVNPTTYIKNLSDFYNEKYKF"
BASE COUNT          443 a          184 c          150 g          505 t
ORIGIN      
        1 gagattttat atttccaatt ttagtaatca gattttatca ataaattact attctattaa
       61 tttaattaaa taacctttaa atttaattat aaaaactgtt gcagaaattt ctacaacagt
      121 ttaaaaaatt taccttacaa aaacttattt tataacaaga gagtatatta taaggctagt
      181 acagcccatt ttttgcactg agaaattctt ttgccatcat caccttgttc tttgtcatat
      241 gcaaattgat taagcatttc acatggaaat gtatcacata gaccacaatg tgtaaggttt
      301 ttatcttcac aacaagattt tacaggacat tgttcacccc aaaatggttt ctcaatctct
      361 gtacaacctt tacaattgac tttttccttg tatccacatt cataacataa aattccacat
      421 cttgattcta tcattttaag tcactcctta aatataaatt ttatatgatt gtatatcaaa
      481 atttatactt ttcattgtaa aaatcggaca aattttttat ataggttgtt ggattaacac
      541 cacaaataga tttaaaatca tttataaaat gagattggtc gtaataacca atatcttgtg
      601 ctaaagttgc aaatggtatt ttattatttt gtatttcttg taatacaagg tttatacgaa
      661 gtaatctaag gtatgattta acactcatac caagagaatt attaaaaatt ctatttaaat
      721 gtcgttcact ataacaacta atttgtgaaa tgtttttaac agacataata gagttttgtt
      781 ttctagcatt ttcaagaatt ggtatagtca tatctactat atttgatttc aataagtatg
      841 ataagaataa catatctaat tgttctacta attcctttat agtacttgtt ctttcaaaaa
      901 tggaattaac ctctaagcta aataatgtgt taaaatcttc aagtgaaatt tttaaatctg
      961 tagattctct ttgagaaaga cctgtcaaat aatatactcc acctgggcga aactcaacga
     1021 atactctaaa aagtacattt tctatatcat tttttacaat agtagttttt gttgtggctc
     1081 cccagaatgc gctttctata ccttttttgt caaatttaaa tatcatacat ccactagcat
     1141 caggtattaa tgtaagattt tctttaatac ttgtatctgg aatagatatt gtatagtgtg
     1201 ctatatactg tcttaacaat ttatgtggca aaatatatgt atactgtttg caggtattta
     1261 aaatgttatt gattatttta tt
//
LOCUS       DAMJYR010000286         1263 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.291, whole genome shotgun sequence.
ACCESSION   DAMJYR010000286 DAMJYR010000000
VERSION     DAMJYR010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1263)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1263)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1263
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.291"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(83..1123)
                     /locus_tag="PC471_003902"
     CDS             complement(83..1123)
                     /locus_tag="PC471_003902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HDF2790945.1"
                     /translation="MNLNLTNTINLMKKYLDIPSPAGYTENAVLEIKKDFENLSLDTI
                     LTKKGALIATLPGEDDSNQITISAHMDTLGAIVKEISSDGTLKYHKVGGGCWAAIEGE
                     NCTVITRKGKKIRGSVVFKYASTHIYGQTKAATERNEETMIIRLDEKVFTKQDVLDLG
                     ISVGDFVCLDHRFEATESGFIKSRYIDNKSAVAMVLEICRYFKENNLTPKHTTNFYIS
                     NYEEIGHGVSKCLPEKTKEFVAIDIAPTGPSQTSSEHGVTIVAKDNLSLYDFNLRNKL
                     VDVAEDNNLDYAVDVFTFYGSDASEAIRWGEDVQIACVGPGTNNSHHYERTHIEAIEN
                     TLKLLINYMLKE"
BASE COUNT          432 a          197 c          146 g          488 t
ORIGIN      
        1 tagaaaaacc aatttttgta taaaaataaa aattaaagtt aattatttgt taagaattga
       61 ctttggtaaa atatataatt aattattctt taagcatata atttataagt aattttagtg
      121 tattttcaat agcttctata tgtgttcttt cataatggtg actattgttt gttccaggac
      181 caacacatgc tatttgaaca tcttctcccc aacgaatagc ttctgaagca tctgaaccat
      241 agaaagtaaa tacatcgaca gcgtagtcta aattattgtc ttctgcaaca tcgacaagct
      301 tgtttcttaa attaaaatca tataatgata aattatcttt tgcaactata gttacaccat
      361 gttcactaga tgtttgactt ggtccagtag gtgctatatc tattgctaca aattcttttg
      421 ttttttcagg taaacattta gaaacaccat gtccaatttc ttcataatta cttatataaa
      481 agttagtagt atgcttaggt gttaaattgt tttctttaaa atatctacat atttcaagaa
      541 ccattgctac agctgattta ttatctatat atctagattt tataaaacca gattctgtag
      601 cctcaaatct atggtctagg catacaaaat ccccaacact tattcctaaa tctaatacat
      661 cttgctttgt aaatactttt tcatcaagtc taataatcat tgtttcttca tttctttcag
      721 tggcagcttt tgtttgacca tatatatgag tagaagcata cttaaataca acacttcctc
      781 tgattttttt accttttcta gttataacag tacagttttc tccctctata gccgcccaac
      841 atccaccacc aactttatgg tatttaagag taccatcaga tgatatttct tttactatag
      901 ctccaagtgt atccatatgt gcagatattg ttatttgatt tgaatcatct tcaccaggaa
      961 gagtagctat aagtgcacct ttttttgtaa gaatagtgtc aagacttaaa ttttcaaaat
     1021 cttttttgat ttctaaaact gcattttcag tatatccagc tgggcttgga atgtccaaat
     1081 attttttcat taaatttata gtattagtta aatttaaatt catattgttt cctcctaaat
     1141 aaatatattt atacaaatta taacataaat acaaatgttc tgaaaactaa aattaaaaga
     1201 atagaatcta aacatattta aaaaaaaagt atataaaata aatttgattt atattatata
     1261 gat
//
LOCUS       DAMJYR010000287         1224 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.292, whole genome shotgun sequence.
ACCESSION   DAMJYR010000287 DAMJYR010000000
VERSION     DAMJYR010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1224)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1224)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1224
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.292"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(148..960)
                     /locus_tag="PC471_003903"
     CDS             complement(148..960)
                     /locus_tag="PC471_003903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDF2790946.1"
                     /translation="MYLERPELYKQSEINFWDDEYISEQLLKAHLDTNFEGASRNFNF
                     IEDSVNWIVTVANPVNYPKLLDLGCGPGLYAEKFAQKGYKVTGIDFSKRSINYAQNRN
                     KETNLNINYLFQSYLNMNYNEEFDLATLIYCDYGALSTENRRLLMEKIYDSLKPGGKL
                     ILDVFTINKYNNFEEIKYWEINEDGGFWSNEKYMCLQDNCKYNDYNTLEQTLVITEKD
                     ENVFYVWNHYFSKESFLLEVENIGFKSVEFFSNVKGEAYSDDSMTMGLVLQK"
BASE COUNT          441 a          197 c          101 g          485 t
ORIGIN      
        1 ataattcaag tattgatttt tatataaaca aatttgtaat gtactaaaaa taacataaaa
       61 gtttccattt ttcaatatgt aagcttttct tatgttattt tattgttaaa ggtttcaagt
      121 ttttatttat tcattttaaa aaaattatta tttttgtaaa actaatccca tagtcatact
      181 atcgtcagaa tatgcttcac ccttaacatt actaaaaaac tcaacagact taaatccaat
      241 attttcaact tctaataaga aactttcttt tgaaaaatag tgattccaaa cataaaatac
      301 attttcatct ttttcggtta ttacaagagt ttgttctaat gtattataat cattgtattt
      361 acaattatct tgtagacaca tatatttttc attactccaa aatcctccat cttcatttat
      421 ttcccaatat ttaatttcct caaaattatt gtatttattt attgtaaata catctaatat
      481 taattttcct ccaggtttta aactatcata aattttttcc attaataacc ttctattttc
      541 agttgacaaa gctccataat cacaataaat taaggtagcc aaatcaaatt cctcattata
      601 gttcatattt aaataacttt gaaacaagta atttatattt aaattagtct ccttatttct
      661 attttgtgca tagttaatag aacgttttga gaaatcaatt ccagttacct tataaccttt
      721 ttgtgcaaat ttctctgcat ataaacctgg accacaacct aaatcaagta atttaggata
      781 attaactgga tttgcaactg taactatcca atttacagaa tcctcgataa agttaaaatt
      841 tctacttgct ccttcaaaat tagtgtctaa gtgtgctttt aataattgct ctgaaatata
      901 ctcatcatcc caaaaattaa tttcactttg tttataaagc tctggtcttt ctaaatacat
      961 acataatttg tttaacaaaa cataccactc cttaaaattt atttttaaaa taaaaaaacc
     1021 acagagttta ctctgtggtt taacttcaaa aagatgagtt taaataagag taaaatagaa
     1081 ccaaatattt ttaagcacaa caataaaact atagctttac tttgtttgct tattaaattt
     1141 gattttattc actcttttcc agcaatactt atatcaacga ttttatgata tcaaatatca
     1201 ccgaacaaca tcatcctttc atat
//
LOCUS       DAMJYR010000288         1192 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.293, whole genome shotgun sequence.
ACCESSION   DAMJYR010000288 DAMJYR010000000
VERSION     DAMJYR010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1192)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1192
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.293"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(316..1062)
                     /gene="cdtR"
                     /locus_tag="PC471_003904"
     CDS             complement(316..1062)
                     /gene="cdtR"
                     /locus_tag="PC471_003904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family response regulator CdtR"
                     /protein_id="HDF2790947.1"
                     /translation="MDILIFDNDVCFGMKLKEKINNILIKEGFDNDVVRLYHNANLLL
                     KELTEKNKVRIYFIVVDAKYKISNELCDGLWIAQKIRESDYISPIIFLTNHIEMILGI
                     FDYRLEVMDFILKHDMEIAESKIKACIKIAHKRYIKEKNYRSNFFTIYSDFSLWKISF
                     DEVIYFETSTIPHKIKLVTTSRIFEFYKSLKSLPDLDACFIRVHKSFVVNKYHIVSLD
                     LKKNNIKMSNGHICRISNTYRNILKNIIKT"
BASE COUNT          445 a          141 c          123 g          483 t
ORIGIN      
        1 aattttttat ataaatttca taaaatatgt aggtatatat cataaaaata tgtatttata
       61 tgatatgtca gtacaaaata acaactgaat attttaaaga gactatttcg tacttttatg
      121 atttaatgag ctatcttatc gaatcataat ctaggaacaa ttgttataat atctaatagt
      181 agttatatat gatatagtca gtatgtgttt tttcacaata tgattattat atagaggatt
      241 cttagggatt actctttttg tataaaaaag agatggttca aatttttatc ttgagacatc
      301 tcctttttct atttattatg ttttaataat attctttaaa atatttctat atgtattaga
      361 tatacgacat atatggccat tactcatttt aatattattt ttctttaggt cgagagaaac
      421 aatatgatat ttattaacta caaaagattt atgtacacgg ataaagcatg catctaaatc
      481 tggtaaagac tttaaacttt tatagaattc aaatattcta gaagtagtca ctaattttat
      541 tttatgtgga atagtgcttg tttcaaaata tataacttca tcaaatgaaa ttttccatag
      601 tgagaaatct gaatagatag taaaaaaatt agagcgataa tttttttctt taatgtatct
      661 tttgtgagca attttaatac aagcttttat tttactttca gcaatttcca tatcatgttt
      721 tagtataaag tccataactt ctaacctata atcgaagatc cctaaaatca tttctatatg
      781 atttgtaaga aatatgattg gactaatata atctgattct ctaatttttt gtgctatcca
      841 taatccatca cataattcat tacttatctt atattttgcg tctacaacta taaaatatat
      901 tcttacttta tttttttcag taagctcttt taataacaaa tttgcattat gatataatct
      961 tacaacatca ttatcaaaac cttcttttat taaaatatta tttatttttt cttttagttt
     1021 cattccaaaa caaacatcat tatcaaagat taatatatcc actaaatacc ctcctataaa
     1081 aaattcaaaa gtaaaaaaac aaaagtattt tatagaaaat acataaaata acactaggag
     1141 ataaataaga ttaaggatag ttagaattaa ggtatattta tgcttgttta ta
//
LOCUS       DAMJYR010000289         1112 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.294, whole genome shotgun sequence.
ACCESSION   DAMJYR010000289 DAMJYR010000000
VERSION     DAMJYR010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1112)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1112)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1112
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.294"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            297..977
                     /locus_tag="PC471_003905"
     CDS             297..977
                     /locus_tag="PC471_003905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2790948.1"
                     /translation="MQIDVLIIDGDKNSSQELKNFLEGKGINVYLAYNCKDAIIKIFS
                     KKFDLIFLEIILVDGDGWDFCKKIRNITTCPIIHITYINENQSILNALNSGGDDYLIK
                     PLNLEILYAKVKAILRRRDLFLNNNKNNIKLEDYNRASGVIKLENKMIKLTPTENRLL
                     NYFIENAEKTLTIKEIYEHVWMNEYLDDNYSVVVAVSGLRKKIEKDYKNPQKIITIRE
                     VGYYFNKI"
BASE COUNT          492 a           68 c          173 g          379 t
ORIGIN      
        1 ataaaaaata tagctttgaa ttatataatt gtatatgctt atctttatac agtaaataaa
       61 aaatcaaatg aaataccaaa taaagtgtat atcttaaaat aacttactag agatacaagg
      121 atgctatgtg aattttgaag tgtgcagttt agataataaa tatatataaa taataaagaa
      181 aatggattta tagtatttaa aatttcttat aagggtaaaa aatgctatta gaaaatgtac
      241 taaaaatatt tttccaattc tttatagaaa aataatgata tttaggagag attgtaatgc
      301 aaattgatgt attgataatt gatggagata agaatagttc tcaagagctg aaaaattttt
      361 tagaaggaaa ggggataaat gtctatttag catataattg taaagatgct attataaaaa
      421 tatttagcaa gaaatttgac ttaatatttt tagagataat tttagttgat ggtgatggtt
      481 gggatttttg taaaaaaatt agaaatataa ctacttgccc aattattcat ataacttata
      541 taaatgaaaa tcaaagtatt ttaaatgctt taaattctgg aggagatgat tatttaataa
      601 agcctttaaa tttagaaata ttatatgcga aagttaaagc tattttaaga agaagagatt
      661 tatttttaaa taataataaa aataatataa aactagaaga ttataataga gctagtggag
      721 taataaagtt agaaaataaa atgataaagt taactccaac agaaaataga ttattaaatt
      781 actttataga gaatgctgaa aagactctaa ctattaagga gatatatgaa catgtatgga
      841 tgaatgagta tttagatgat aattatagtg tagtggttgc tgtgagtgga ttaagaaaga
      901 aaatagagaa agattataaa aatcctcaaa aaattataac gataagagaa gtagggtatt
      961 actttaacaa gatataaagc tttataaaca gaatgaatga taaggcatag ataatatgtt
     1021 agtatattat aagagttttt gttctaacga tatatttgta aaattagatt taatttactt
     1081 aattaaatta tagcagtaga ataaatataa aa
//
LOCUS       DAMJYR010000290         1095 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.295, whole genome shotgun sequence.
ACCESSION   DAMJYR010000290 DAMJYR010000000
VERSION     DAMJYR010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1095)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1095)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1095
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.295"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(216..1085)
                     /locus_tag="PC471_003906"
     CDS             complement(216..1085)
                     /locus_tag="PC471_003906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HDF2790949.1"
                     /translation="MVIIKAIELYKKLDLEFNIKNINDDWSFMNFENDFITPEFRKKY
                     IGLVLDNAQNINKVYTTTFPDKEIIKQVIDKDEKDILIFSHHAMGYIASDEGFPFHDI
                     PLSYMEEMKNRRISFYVLHSPLDNYSDYSTSVSFAKAMGLEIVKPFCKYDDKIEVGVI
                     CKTSLKSIEEIKELIKKSVGHDVKLYDYGDSVLKDGLVAIAAGGGSYPFVAREVAELG
                     INLYITGFTKPLKHFEPVLEFHQIAKDNLINVIGATHYSTEKFACMGMVDYFKNLGLE
                     SEFLQGKYYLEDL"
BASE COUNT          411 a          171 c          104 g          409 t
ORIGIN      
        1 ataatctaaa atacatcaaa aaataaacta aaaaatatcg aaaaataatt taaaagacat
       61 caaaactaaa tcttctaatc agcaattaaa agtctatttt cttagtttaa aaaataaaga
      121 tagatttttt caaaacactg gataaatagg taaaaaaata aacagttagt aaaataaatt
      181 tttactaact gtttattttt attgtttaat atgtcctata aatcctctaa gtaatatttt
      241 ccttgcaaaa attctgattc taaacctaga tttttaaaat aatcaaccat gcccatacac
      301 gcaaattttt ctgtagaata atgagtagca cctataacat taattaaatt atcttttgct
      361 atttgatgga actctaatac tggttcaaaa tgttttaaag gcttagtaaa cccagtaata
      421 tatagattta taccaagttc agcaacttct cttgctacaa atggataact tcctccccct
      481 gctgcaatag caactaaacc atcttttaat acagaatctc cataatcata caatttcaca
      541 tcatgaccaa cagatttttt aattaattcc ttaatctctt ctattgattt tagtgatgtt
      601 ttacatataa ctcctacctc aattttatcg tcatatttac aaaatggttt gactatttct
      661 aaacccatag cttttgcaaa actaacagaa gttgaatagt cactatagtt gtctagtgga
      721 gaatgtaata cataaaaaga aattctacga tttttcatct cttccatata agatagtggt
      781 atgtcgtgaa atggaaaacc ttcatctgat gctatgtatc ccattgcatg atgtgaaaaa
      841 attaaaatat ctttttcatc tttatctata acttgcttaa ttatttcttt atcaggaaaa
      901 gtagttgtgt atactttatt aatattttga gcattatcta atactaatcc tatgtatttt
      961 tttctaaatt ctggagtaat aaaatcattt tcaaaattca taaaactcca atcatcatta
     1021 atatttttaa tattaaattc taaatctaat tttttataaa gttcaattgc cttaataata
     1081 accactcctt tataa
//
LOCUS       DAMJYR010000291         1091 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.296, whole genome shotgun sequence.
ACCESSION   DAMJYR010000291 DAMJYR010000000
VERSION     DAMJYR010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1091)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1091)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1091
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.296"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(434..610)
                     /locus_tag="PC471_003907"
     CDS             complement(434..610)
                     /locus_tag="PC471_003907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2790950.1"
                     /translation="MFIGEIDKCTHILTAYISSSYDYCNFLDTQLDDFISEYGETVVE
                     TCLHQVLLLVSRYN"
BASE COUNT          434 a          120 c          129 g          408 t
ORIGIN      
        1 gtattattta tataaatatt gaaatttgtc tagttatacg tttttattga ccagttatgc
       61 agttgatatt gaatattcta aatattggtg ttattttata ggtggtttaa acatattagg
      121 gggattttat atatgatttg tatgatttat agagaatgta taaaatagta aaggtttaga
      181 gccatgttta gtggattgca gaatatgcat atagaatgat tcttgtaaat taagttagac
      241 ataggaaata tataatttgt attggatgat taattttatg gtgtatgctt ttaacttcta
      301 taatgggaaa gtaaagatgt actcccaata catctttact ttagcagaag ttatagttac
      361 ttataaagta caaaaaatta tatatttatt agagtagata aatctaaaaa ggtttttcta
      421 gaaaaattta gttttaattg tatctactta ctaataataa gacttgatgt aagcaggttt
      481 caactaccgt ttctccatat tctgatataa aatcatctaa ctgtgtatct aaaaaattac
      541 aataatcata tgaactactg atgtaagcag ttagtatatg tgtacatttg tctatttctc
      601 caataaacat attatccatc cccctaaaaa atttagtaaa aataaaaagt cgcattctat
      661 tagtcaatta acataattta taaaattttt tagtactagt ttctataact taactcctta
      721 aggttaaata ttcccagttt taccaattta gttttattct acaagtatat aatactagaa
      781 gatgctccat tattcaacat gcttgtttga gaatagaaaa tattctaaaa ttataattgt
      841 ttttaaaaat aagattcaag ataaataaag ttatatgcaa ctatttaata atctttccat
      901 aataaggaca gtaggttcta ttcttaaaaa actccatctt taaatttaaa gaataactac
      961 aatttgaaca acaataaaat ttccaatcaa taaaaatatc aaaaataata aaaataattg
     1021 aaaatgtata atttcccaga aaaataataa atgtaactcc aataataaac acacctataa
     1081 attataaaat a
//
LOCUS       DAMJYR010000292         1083 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.297, whole genome shotgun sequence.
ACCESSION   DAMJYR010000292 DAMJYR010000000
VERSION     DAMJYR010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1083)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1083)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1083
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.297"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            59..1015
                     /locus_tag="PC471_003908"
     CDS             59..1015
                     /locus_tag="PC471_003908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CorA family divalent cation transporter"
                     /protein_id="HDF2790951.1"
                     /translation="MYILNLNTRETIEDFRDKFYVAENSYLILSAPKNLKLLKETLDI
                     DEITFNDCLKFDEITKLDLFDNYDFLSLNTFELRDGEAVIEEVNMYLSDNFILVVVNE
                     EHFLFEFVKNIILKNSQLEKNPVINLFKINYLILREVIKNGFESLEKVEELILQIEDE
                     MMDNINKNHVSRISDVRGLTRIIVKNTRPLLYIGDRIVKENIRYLKYSNVKKYNLENF
                     QGIDFGIDKLYNFALSTRELADKLLDIYSSRVGEKTNNLITKLTLLTAISAPLTIITG
                     IYGMNFRYMPELNWIYGYPATLFFMLCIIFVGIIIFKIKKLL"
BASE COUNT          446 a           89 c          164 g          384 t
ORIGIN      
        1 ttacagtaag gattataaaa aatttttaga atgtaaattg aaatttagga gtagatgtat
       61 gtatatactg aatttaaata caagagagac tattgaagat tttagagata aattttatgt
      121 agcagaaaat tcttatctta tattaagtgc tccaaaaaat ttaaaattgc ttaaggaaac
      181 tttggatata gatgaaataa cttttaatga ttgtcttaaa tttgatgaaa taaccaaact
      241 tgatttattt gataattacg attttttaag cctaaataca tttgaactaa gagatggaga
      301 agctgttata gaagaggtaa atatgtattt atcagataat tttatattgg ttgtagtgaa
      361 tgaagaacac tttttatttg aatttgtaaa aaatattatt ttgaagaact ctcaacttga
      421 aaaaaatcca gttattaatc tatttaagat aaactacttg attcttagag aagttataaa
      481 aaatggtttt gagtctttgg aaaaagttga agagttaatt cttcaaatag aagatgaaat
      541 gatggacaac attaataaga atcatgtatc tagaataagt gatgtaagag gattaacaag
      601 aattatagtt aagaatacta gacctctttt gtatattggg gatagaatag ttaaggaaaa
      661 tataagatat ttaaaatatt caaatgtaaa aaaatataat ttagaaaatt ttcaaggtat
      721 agattttgga attgacaaat tatacaattt cgcattgtca acacgagagt tagcagataa
      781 attattagat atttattctt ccagagtagg tgagaaaaca aataacttaa ttacaaaatt
      841 aaccttgtta acagccatat cagcaccact tacaattata actggtatat atggaatgaa
      901 ctttagatat atgccagaat taaactggat ttatggatat ccagcaactt tattttttat
      961 gctatgtata atttttgttg gaattataat atttaaaata aaaaaattat tgtaaattac
     1021 agatgatgtt taattgattt tataaaaata catcaattga gaagctgatt taagtaaata
     1081 aaa
//
LOCUS       DAMJYR010000293         1082 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.298, whole genome shotgun sequence.
ACCESSION   DAMJYR010000293 DAMJYR010000000
VERSION     DAMJYR010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1082)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1082)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1082
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.298"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            7..81
                     /locus_tag="PC471_003909"
     tRNA            7..81
                     /locus_tag="PC471_003909"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:39..41,aa:Asn,seq:gtt)
     gene            87..172
                     /locus_tag="PC471_003910"
     tRNA            87..172
                     /locus_tag="PC471_003910"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:121..123,aa:Leu,seq:taa)
     gene            188..263
                     /locus_tag="PC471_003911"
     tRNA            188..263
                     /locus_tag="PC471_003911"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:221..223,aa:Met,seq:cat)
     gene            271..345
                     /locus_tag="PC471_003912"
     tRNA            271..345
                     /locus_tag="PC471_003912"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:304..306,aa:Glu,seq:ttc)
     gene            360..433
                     /locus_tag="PC471_003913"
     tRNA            360..433
                     /locus_tag="PC471_003913"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:392..394,aa:Gly,seq:tcc)
     gene            439..514
                     /locus_tag="PC471_003914"
     tRNA            439..514
                     /locus_tag="PC471_003914"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:472..474,aa:Val,seq:tac)
     gene            520..596
                     /locus_tag="PC471_003915"
     tRNA            520..596
                     /locus_tag="PC471_003915"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:554..556,aa:Asp,seq:gtc)
     gene            607..681
                     /locus_tag="PC471_003916"
     tRNA            607..681
                     /locus_tag="PC471_003916"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:639..641,aa:Gly,seq:gcc)
     gene            691..764
                     /locus_tag="PC471_003917"
     tRNA            691..764
                     /locus_tag="PC471_003917"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:725..727,aa:Arg,seq:tct)
BASE COUNT          306 a          229 c          252 g          295 t
ORIGIN      
        1 aaatgttcca aggtagctca acggtggagc aaccggctgt taaccggtag gctgtgggtt
       61 cgagccccac ccttggagcc aaaaaagccg aagtggcgga actggcagac gcacaggact
      121 taaaatcctg cgggacttac ctctcgtacc ggttcgattc cggtcttcgg cacttataaa
      181 acaatatcgc ggggtggagc agttggcagc tcgtcgggct cataacccga aggtcgtagg
      241 ttcaagtcct gcctccgcaa ccattaaaac ggctcgttgg tcaagaggtt aagacaccgc
      301 cctttcacgg cggtaacagg ggttcaattc ccctacgagt caccaattca attaaatatg
      361 cgggtgtagc tcaatggtag agttctggcc ttccaagcca gctgtgaggg ttcgatcccc
      421 ttcacccgct ccaaaaatgg gaccatagct cagctgggag agcacctgcc ttacaagcag
      481 ggggtcacag gttcgagccc tgttggtccc accattcgcg gcctggtagt tcagttggtt
      541 agaatgccag cctgtcacgc tggaggtcga gggttcgagc cccttccagg tcgccaaatt
      601 aaataagcgg gaatagttca gtggtagagc gcaaccttgc caaggttgaa gtcgcgagtt
      661 cgaatctcgt ttcccgctcc ataataactt gggtgcatag ctcagctgga tagagcaacg
      721 cccttctaag gcgtgtgtcc ggggttcgaa tccctgtgcg ctcattaaga aaagtttact
      781 ataaattata gtaaactttt atattgttta aaaagacaca ggaaacatgt atattacctg
      841 tgtcttttta tttataaact ttaaactatg taaaaattac agtatattct aatagagtta
      901 cagtatattc attaatattg taaacatacg gagggtaatg tataatagtc tacaagatgt
      961 ttatgaaggc atcagttaaa tataataaat ataactaatt agtaagagtt atatacatta
     1021 tattgtatac tttatggtat ttatatattg aaataactta tgaaattatt tataaagaga
     1081 tt
//
LOCUS       DAMJYR010000294         1072 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.299, whole genome shotgun sequence.
ACCESSION   DAMJYR010000294 DAMJYR010000000
VERSION     DAMJYR010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1072)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1072)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1072
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.299"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(224..715)
                     /locus_tag="PC471_003918"
     CDS             complement(224..715)
                     /locus_tag="PC471_003918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbM family protein"
                     /protein_id="HDF2790952.1"
                     /translation="MGMIGCYTKISEENVFKLQQAEENLQDFVFEDANENSTINIDKA
                     WHAIHFTLTGCPFGGDDDNIFSKLVLSGNILMEIDGEFPVMLITANDVKKLSKAMNSL
                     EEQAFRKRFNINEMLENNIYPVMNDESEDDFFEYVWANLIELKKFIQEASDDGLAVIF
                     FIM"
BASE COUNT          412 a          158 c           92 g          410 t
ORIGIN      
        1 gataaataaa aatatctaca actatatatt gcaaaaattt aaaactaatt tatatatgct
       61 aatataaata tttataaaga cataagacaa atttacctac agatttaaat ttaacttata
      121 tattttaaaa ggctaatata atatgtattt tcttctatac aatcaaaagg cttcatatct
      181 aagatatgtg atgacactaa tattcacaat tttttagtaa ttatcacatt ataaaaaata
      241 ttacagctag tccatcatca gatgcttctt gtataaactt tttaagctca attagatttg
      301 cccatacata ttcaaaaaaa tcatcttcac tctcatcatt cataacagga taaatattat
      361 tttctaacat ctcattaata ttaaatcttt ttctaaatgc ttgttcttcc aaactgttca
      421 ttgcttttga tagttttttt acatcatttg ctgtaatcaa catgacagga aattctccat
      481 caatttccat aagtatgttt ccacttagta ctaatttgct aaatatatta tcatcatctc
      541 caccaaaagg acaacctgtc aaagtaaagt gaatggcatg ccatgcttta tcaatattga
      601 tagtactatt ttcgttggca tcttcaaaaa caaaatcttg taagttttct tctgcttgct
      661 gtaatttaaa aacattctct tcacttattt tagtataaca tcctatcatc cccataatta
      721 cattcctcct aagtagcgtt tttttaattg taacttataa ttaaacttag ttacaataaa
      781 atatattgag aaaagtatta tttcatctta aatttaaata cctatattat caactaaaat
      841 tctgtgttta tagcgtaagt attattattt tttcctaaaa attaaatgaa tcagaagttc
      901 tataggcaat attgactata aataagaact attactttta aaaaaaaatt taagaaatta
      961 tactgtagaa tcatgctcta cttattagct ttaaataaaa aataaatcat gttctcaaat
     1021 taaataataa ataacaataa atttaaattt taaactataa gagtttctga ag
//
LOCUS       DAMJYR010000295         1003 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.300, whole genome shotgun sequence.
ACCESSION   DAMJYR010000295 DAMJYR010000000
VERSION     DAMJYR010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1003
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.300"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            106..669
                     /locus_tag="PC471_003919"
     CDS             106..669
                     /locus_tag="PC471_003919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2790953.1"
                     /translation="MNKVYIVTTYTGTILSYLIRNISKKLYTHVSISLNENLKPMYSF
                     GRLNPRNPFIGGFVEENINQGLYAIRKNTVCRVYSLEVDNLQYENLYKNIKLISDYRE
                     DYYYDTMALIYLLMNIQRQSEYKYVCSNFVADMLEKSEIDILNKQAFEVTPNDFYNLS
                     GLTLEYEGLLSEYNSRQHSYVSKIANI"
BASE COUNT          412 a           97 c          143 g          351 t
ORIGIN      
        1 ccttaaaatc aaaatttgta aaaccacatt tattatgtta gaatatctta tgatattaaa
       61 ttgtaagtgt ataatattat ttagttacta gattgggggg aaatgatgaa taaagtctat
      121 attgtaacaa catatacagg aactatatta tcttacttaa taaggaatat aagtaaaaag
      181 ctgtatacac atgtgtcaat atcattaaat gaaaatctaa aaccaatgta ttcttttgga
      241 aggctaaatc caaggaatcc atttattgga ggatttgttg aagaaaatat aaatcaagga
      301 ttatatgcta taagaaaaaa cactgtgtgt agggtatatt cgttagaagt agataattta
      361 caatatgaaa atctatataa aaatataaaa ttaattagtg attatagaga agattactat
      421 tacgatacaa tggcattaat atatttacta atgaatattc agagacaatc tgaatataaa
      481 tatgtatgtt caaattttgt tgctgacatg ttagaaaaaa gtgagataga cattttaaat
      541 aaacaagcat ttgaagtaac gccaaatgat ttttataatt tatctggctt aaccttagag
      601 tatgaaggtt tattatcaga atataattct agacaacatt cttatgtatc aaaaatcgca
      661 aacatttaaa aacttgattt agagtttttc atataaaaaa atattgctag ataattaggc
      721 tgaagatagt agtttaactt cgataggatt aagaatttgt aaatcttagt tagaatactt
      781 tcttcagttt ttaaatttct gctaatattt gtatttttat aacatttttg gatatatata
      841 atacagaata gtgcaaaaat cccactcctt taggtgcggg atggatagca caaacagtgt
      901 ataaaaagta tttatacata cacgtataat gttaaatata gttgaataag cagaactata
      961 aatatataaa gatatcttaa tatataataa atatgataaa tat
//
LOCUS       DAMJYR010000296          987 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.301, whole genome shotgun sequence.
ACCESSION   DAMJYR010000296 DAMJYR010000000
VERSION     DAMJYR010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 987)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 987)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..987
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.301"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            130..384
                     /locus_tag="PC471_003920"
     CDS             130..384
                     /locus_tag="PC471_003920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2790954.1"
                     /translation="MENKDFKLTICIFAFVIGVVVLFCCIGLGEFSAEHIIQQNGGIM
                     DTDQYNIYLDQSISQYRNFGSILALLGGIGILFDKYIRKY"
     gene            429..752
                     /locus_tag="PC471_003921"
     CDS             429..752
                     /locus_tag="PC471_003921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2790955.1"
                     /translation="MATEIFTVKITPLEAQKMIKENLDADLVFSDSYNLGDDKFILIT
                     TFEKYYARNNSDAGLIVVYENTTGKTVIKATSTGSAIGILKIDWGAGGNLLKRVKNIL
                     ADYII"
BASE COUNT          409 a           79 c          156 g          343 t
ORIGIN      
        1 ttaaatatat aaaactataa aggttacaat tttggaaata gatgtaaata tatgttgtat
       61 tgtataataa gagtaatata aaatctagta aaaataatca aataaattta gcttatgaga
      121 gggataacta tggaaaataa ggattttaaa ttaacaatat gtatatttgc ttttgtaatt
      181 ggagtagtag tattattctg ttgtataggt ttaggtgaat ttagtgcaga gcatattatt
      241 caacaaaatg gtggaatcat ggatactgac caatataata tttatctaga ccaatctatt
      301 tctcaatata gaaattttgg ttctatatta gctctgttag gaggaatagg gatattgttt
      361 gataaatata tcagaaaata ctagagatta tgttttgtct aaaataaatt cttatgagga
      421 gataaaaaat ggcaacagaa atatttactg taaaaattac acccttagag gcacaaaaaa
      481 tgataaaaga aaatcttgat gcagatttag tttttagtga ttcttataat ttaggagatg
      541 ataaatttat actaattaca acttttgaga agtattatgc aagaaataat agtgatgcag
      601 gattaatagt agtttatgaa aataccacag gaaaaacagt tattaaagct acttcaacag
      661 gcagtgcaat tggaatttta aaaattgatt ggggtgcagg tggaaattta cttaaaagag
      721 taaaaaacat acttgctgat tatattattt aattatttta tctaactgat aatatgtcaa
      781 gaaaatctcg taaaagaggt taagaacatt tttataatat atagataaat aagaatttga
      841 aggtaaagtt tttaaggtat aaagaaaaat aatataataa aacgaattgg ctttataatt
      901 ctttataagg caaagagcac atttctttat ataattgttg gatattaaat aatatgtaaa
      961 tacaccattt acatataatt aatataa
//
LOCUS       DAMJYR010000297          975 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.302, whole genome shotgun sequence.
ACCESSION   DAMJYR010000297 DAMJYR010000000
VERSION     DAMJYR010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 975)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 975)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..975
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.302"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            203..>975
                     /locus_tag="PC471_003922"
     CDS             203..>975
                     /locus_tag="PC471_003922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HDF2790956.1"
                     /translation="MADCNSCPSKGNCNSQSNCSIENNPNNKFGKIIGVMSGKGGVGK
                     STVTALLANKLNKMGYKVGILDSDITGPSIPRLMGVKNVKAYSDGSYIYPVENSNNIK
                     VMSINLMIDDENEPVVWRGPLLGGVVKQFYTDVLWEELDYLLIDMPPGTGDVALTVMQ
                     SIPISGIVMVSVPQDLVSMIVSKAVNMAKKMNINVLGVIENMSYIQCPDCSKKIKLFE
                     GESTEKFLDDLDLELLGELPMTKEIIDITHNGVTEISDD"
BASE COUNT          351 a          107 c          174 g          343 t
ORIGIN      
        1 ttctataact aaattgaaat aatcactttt tcaagaatgt tataattttt ttattgtatt
       61 ctttttgcta tagtctatga ataaatcaat agaaaatctt ctaagttgct tttaaataat
      121 gactttatgg ttcaattgta ctaaaatagt atatgtaggt tacaaataat ctaataaata
      181 ttaaagtttg gaggtaatag atatggcaga ttgtaattca tgtccatcaa agggaaattg
      241 taatagtcaa tcaaattgtt ctattgaaaa taaccctaac aataaatttg gaaaaataat
      301 tggtgttatg agtgggaagg gtggagttgg aaaatcaact gtaactgctt tacttgctaa
      361 taagctaaac aaaatgggat ataaggttgg tatattagac tcagatataa ctggaccaag
      421 tattcctaga cttatgggtg taaaaaatgt aaaagcttat tctgatggct cttatattta
      481 ccctgtagaa aactcaaata acataaaagt aatgtctata aaccttatga tagatgatga
      541 aaatgaacct gttgtttgga gaggaccttt acttggaggg gttgtaaagc aattttatac
      601 tgatgttctt tgggaagaat tagactattt attaatagat atgcctcctg gtactggaga
      661 tgttgcctta acagttatgc aatctattcc tataagtgga atagttatgg tttcagtacc
      721 tcaagatttg gtatctatga ttgtatcaaa agctgttaat atggctaaaa aaatgaacat
      781 aaatgtcctt ggagttattg aaaatatgag ttatatccaa tgtcctgatt gttctaaaaa
      841 aatcaagtta tttgaaggag aatctactga aaagttttta gatgatttag acttagagct
      901 tttaggagaa cttcctatga ctaaagaaat tatagatatt actcataatg gagttacaga
      961 aattagtgat gattt
//
LOCUS       DAMJYR010000298          966 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.303, whole genome shotgun sequence.
ACCESSION   DAMJYR010000298 DAMJYR010000000
VERSION     DAMJYR010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 966)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 966)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..966
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.303"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(781..951)
                     /locus_tag="PC471_003923"
     CDS             complement(781..951)
                     /locus_tag="PC471_003923"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2790957.1"
                     /translation="MPPDTETKVTLVFTVTHTSSGKTADTGEIEVTVPARTTTVSSLL
                     GRIAVNIFNFNK"
BASE COUNT          374 a          115 c          101 g          376 t
ORIGIN      
        1 acttctctct tattcaagat ttttctatat aaaatctaag aataaataga aaactaacat
       61 ctaacctttt attttttatt ttcgatctat ttacttgaat tttaacataa atctatcttt
      121 tagtatatat taatcatgct gaatttgtga tgacatgtat acaaaatatc gtttagtatt
      181 ttctaataaa aaatattact gaaattaaaa atctacaata gcatttaaca agtaaaaaca
      241 tgtttacaca aatatataat ttaaggtctt taaaatagag tatgtattat tctaaaaatt
      301 agttatagtt atcacataaa aatagaaaag aattttcaaa agatttaaag aacatatata
      361 cttcaattgc agaagatgta atgttaagtg aactagcatt cttcgaggaa aaatagactc
      421 aaaaaattag aaaatagaat caggttttag atgatttaag tatttatttt caaaaaagaa
      481 atttgattct aagttaataa agaaatattt aaaaaaatca aaaaattttc ttaaataagt
      541 aataggtttt gtaaaaatgg aaaaatatca aaataagact atgattttta aaactatagc
      601 cttatcaata aatttttatt taaagaacac acataaatat ttttaaacct aatttatgtg
      661 aagttttatg tattttttat ataagtagta ttctaatctt actcatctaa tataccaaaa
      721 agactgtaag taagttcact tttatgcaaa cttacccaca atctctttta cacattaatt
      781 ctatttatta aaattaaaaa tatttactgc tattctaccc aataaagatg atactgttgt
      841 tgttctggct ggtactgtta cttctatttc tcctgtatct gctgttttac cacttgatgt
      901 gtgtgtcact gtaaatacta atgttacttt tgtttctgta tctggtggca ctattgttcc
      961 atcttt
//
LOCUS       DAMJYR010000299          942 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.304, whole genome shotgun sequence.
ACCESSION   DAMJYR010000299 DAMJYR010000000
VERSION     DAMJYR010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 942)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 942)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..942
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.304"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            15..875
                     /locus_tag="PC471_003924"
     CDS             15..875
                     /locus_tag="PC471_003924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HDF2790958.1"
                     /translation="MLLKKDRITFSEILILIVGCILMAISLNLFFNPHAIAAGGITGL
                     GVVLNSLFGVELWIVNLLLNVPLFIFAYKILSKKDCFKTVLGIIFLTIALKLTANMAT
                     LDITNDMYLAIISGSILMGVGQGLIFRINGSTGGTDLMALLLNKYFPTFSIPVLMGIV
                     DCVVVVLSGIVNRQVEIALYSTVALYILVKVSDLLIEGFNYSKSFTIISDLSKDISKK
                     IMEDLDRGATILKGEGAYTGENKNVLLVVVEKKEVVELKKLVKNVDPNAFIIITDIHE
                     ALGNGFKKIE"
BASE COUNT          340 a          105 c          149 g          348 t
ORIGIN      
        1 atatataagg agtgatgtta ttgaaaaaag atagaataac tttttcagaa attttaatat
       61 taattgtagg ctgtatatta atggccattt ctcttaactt attttttaat ccacatgcaa
      121 tcgctgctgg tggtataaca ggacttggag ttgttttaaa ttcattattc ggtgtagaac
      181 tttggattgt caatttactt ttaaatgttc cattattcat atttgcttac aaaatacttt
      241 caaaaaaaga ttgttttaaa acggttttag gaataatttt tcttaccata gcacttaagc
      301 ttactgcaaa tatggctaca ttagatataa caaatgatat gtatcttgca attatatctg
      361 gttctatttt aatgggagtt ggtcaaggac ttatttttag aataaatggc tctacaggtg
      421 gaactgattt aatggcactc ctactaaata aatattttcc tacatttagt atacctgttt
      481 taatgggtat tgtagactgc gttgtagttg ttttatcagg tatagtaaat agacaggttg
      541 aaattgcact ttattcaact gtggctttat acattttagt aaaagttagt gacttgctta
      601 ttgaaggatt taattattcc aaatcattca ctattatatc tgacctttca aaagatataa
      661 gtaaaaaaat aatggaagat ttagatagag gtgcaactat tctaaaagga gaaggtgcat
      721 atactggaga aaataaaaat gtacttttag tagtagttga aaagaaagaa gttgtcgaat
      781 tgaaaaaatt agtaaaaaat gtcgacccta atgcttttat tataattaca gatatacatg
      841 aagctttagg taatggattt aaaaaaatag aataactaac aaataaatag caagtataat
      901 taacatatac aaattcaact ttttatatgt tatattatac tt
//
LOCUS       DAMJYR010000300          903 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.305, whole genome shotgun sequence.
ACCESSION   DAMJYR010000300 DAMJYR010000000
VERSION     DAMJYR010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 903)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 903)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..903
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.305"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            147..>903
                     /locus_tag="PC471_003925"
     CDS             147..>903
                     /locus_tag="PC471_003925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HDF2790959.1"
                     /translation="MFAEERIQAILNILKKEGRVTVKELSSKFNVTEDCIRKDLKNIE
                     KISSIRRIYGGAVLARETLENQDTKDRKEINIPTKKIIAEKAFNLIEDRETIFLDIST
                     INILLAKLLAESNKKVTLVTNMLDILNVVTSKPNNLNIISTGGLLNLSLDGFVGTPTI
                     EFISKYKFDKTFMGSCGVDVFNSSLTTFEIEDGLTKKAIINSSKKVFIVMEDKKFKFD
                     GNFKFAHLEDISSIITEKTPTPDIVDILSEFNVN"
BASE COUNT          373 a           90 c          137 g          303 t
ORIGIN      
        1 tttatttact aaattctaaa tatatagtta gaaaaaacat actttttata ttaaattaat
       61 tttgtatttc atgttaaaat agttgatata ttgtatttta tcttataata tttactataa
      121 tactatattg cgaaaagagg tagaaaatgt ttgcagaaga aagaatccaa gctatattaa
      181 atatcctaaa aaaagaaggt agagttactg tcaaagagct cagttctaaa tttaatgtta
      241 cagaagattg tataagaaaa gatttaaaaa atatagaaaa gatttcttca ataagaagaa
      301 tttatggagg agctgtacta gctagagaaa cactagagaa tcaggataca aaagacagaa
      361 aagaaattaa tattccaaca aagaaaataa tagcagaaaa agcatttaat ttaattgaag
      421 atagagaaac tatattttta gatatctcta caattaacat cttgcttgcg aagttgttag
      481 ctgaaagtaa taaaaaagta acacttgtta caaatatgct ggatatacta aatgtagtta
      541 cttcaaaacc aaataattta aatataattt ccactggagg tttattaaat ctcagtttag
      601 acggttttgt cggtactcca actatagagt ttatatcgaa atataagttt gataagactt
      661 ttatgggaag ttgtggagta gatgtattca actcttcact tacaactttt gaaattgaag
      721 atggtcttac gaagaaggct ataataaatt caagtaaaaa agtatttata gttatggaag
      781 ataaaaagtt taaatttgat ggaaatttca agtttgctca tttagaagat attagttcta
      841 taattacaga aaaaacgcct actcctgata tagtagatat tttatcagaa tttaatgtaa
      901 atg
//
LOCUS       DAMJYR010000301          885 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.306, whole genome shotgun sequence.
ACCESSION   DAMJYR010000301 DAMJYR010000000
VERSION     DAMJYR010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 885)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 885)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..885
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.306"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(194..661)
                     /locus_tag="PC471_003926"
     CDS             complement(194..661)
                     /locus_tag="PC471_003926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HDF2790960.1"
                     /translation="MKFLNVLVVVEDIEKSKKFYYDVLGLKVICDFGENVVLEGNISL
                     QEKKLWLEFINKSDSEVKFNGNDAELYFEEDNFDTFVERLSTMKDIDYVHLAIEHRWG
                     QRAIRFYDLDGHIIEVGETMSSVCRRFLDSGLSIDEVAKRMDVTVEYIESVLE"
BASE COUNT          339 a          155 c           56 g          335 t
ORIGIN      
        1 tcttttatat attttttcta aatttaaact ttagtataaa tatataagaa atatcataat
       61 atttttaaaa actcaaataa agctaaacaa gtattatatt ctttatttta gaaaaataaa
      121 aactgtaagc aaatttgttt tcacacaagt ttacttacag ccttcacctc aattttactt
      181 taatataatc catctattct aaaactgatt caatatattc gacagttaca tccattcttt
      241 tagctacctc atctattgaa agaccactat ctaaaaaacg tctacacaca gatgacatag
      301 tttctccaac ctcaataata tgcccatcta aatcataaaa tctaattgct ctttgacccc
      361 acctatgctc tatagctaga tgaacataat ctatatcttt cattgtactc aatctttcaa
      421 caaaagtatc aaaattatct tcctcaaaat ataactcagc atcatttcca ttgaacttaa
      481 cttcactgtc actcttatta ataaattcta accaaagctt tttttcttgt aatgatatat
      541 taccttctaa tacaacattt tctccaaaat cacagattac ctttaacccc aaaacatcat
      601 aataaaactt ttttgatttc tcaatatctt caactactac taaaacattt aaaaacttca
      661 tattacctcc ttaatataca catactttat ttataatata taattagaat taaactataa
      721 aggtttcaat aaggtctagt ataaagaaaa aaattataag tcttattgaa atagttaagt
      781 taattttatc atataatata aattttaatc ttgtaaaaaa cagacatgtt catactttta
      841 ggtttaaatc tatatgtatt cttattaatt ttgctaaaac tgtta
//
LOCUS       DAMJYR010000302          816 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.307, whole genome shotgun sequence.
ACCESSION   DAMJYR010000302 DAMJYR010000000
VERSION     DAMJYR010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 816)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 816)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..816
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.307"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            104..>816
                     /locus_tag="PC471_003927"
     CDS             104..>816
                     /locus_tag="PC471_003927"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2790961.1"
                     /translation="MDRFEVLKYLKFNVWHSEWPLELLKGAITKDNATGNLLLQLKMC
                     NISDKEIKSAYFTIECFNDAGDLIDNNLKYVYQDINIKPHECLGDDIAIKINDNKTRA
                     INIILEQVVFSDDKVHKYEREEGLIIPKLDKIELLNIELLEELKKDYEEYDFQYEIKY
                     MPNIIEKKAWSCCCGKINKLEKDKCDNCKRSKILQIKKINKNYLESIYKKSLKKKKEI
                     EIKLEKDNRHKLKNINKNN"
BASE COUNT          386 a           55 c          120 g          255 t
ORIGIN      
        1 tatatatgtt atcattttta tttaccttat ttttacaaat tattccataa gtatattata
       61 attatcttga gggaaataat catatagagg gagaaattag aaaatggata gatttgaagt
      121 attaaaatat ctaaaattta atgtttggca ttcagaatgg ccattagagc ttctcaaagg
      181 ggctataaca aaggataatg ctactggtaa tttattatta caattaaaaa tgtgtaatat
      241 atctgataag gagataaagt ctgcatattt tacaatagaa tgttttaatg atgcaggaga
      301 tttaatagat aataatttaa agtatgttta tcaagatata aacattaaac cacatgaatg
      361 tcttggtgat gatattgcta taaaaataaa tgataataaa acaagagcaa taaatattat
      421 attagaacaa gtagtatttt cagatgataa agttcataaa tatgaaagag aagaaggttt
      481 aattatacct aaattagata aaatagaatt gttaaatata gaattattag aagaacttaa
      541 aaaagattat gaagaatatg attttcaata tgaaattaag tacatgccta atataataga
      601 aaaaaaagca tggagctgtt gttgtggaaa aataaataaa ttagaaaaag ataagtgtga
      661 taattgtaag agaagtaaaa tactgcaaat aaaaaagata aataaaaatt atttagaatc
      721 tatttataaa aaaagcctaa aaaagaaaaa agaaatagaa ataaagcttg aaaaagataa
      781 tagacataaa ttaaagaaca taaataaaaa taatag
//
LOCUS       DAMJYR010000303          787 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.308, whole genome shotgun sequence.
ACCESSION   DAMJYR010000303 DAMJYR010000000
VERSION     DAMJYR010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 787)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 787)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..787
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.308"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(<1..>787)
                     /locus_tag="PC471_003928"
     rRNA            complement(<1..>787)
                     /locus_tag="PC471_003928"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          172 a          238 c          182 g          195 t
ORIGIN      
        1 cgtccatccc ggtcctctcg tactaaggac aggtcttctc aaatttccta cgcctgcgac
       61 ggatagggac cgaactgtct cacgacgttc tgaacccagc tcgcgtacca ctttaatggg
      121 cgaacagccc aacccttggg acctactaca gccccaggat gtgatgagcc gacatcgagg
      181 tgccaaacct ccccgtcgat gtggactctt gggggagata agcctgttat ccccagggta
      241 gcttttatcc gttgagcgat ggcccttcca cgcagaacca ccggatcact aagtccgatt
      301 ttcatccttg ctcgacctgt atgtcttgca atcaagctct cttttgcctt tacactctac
      361 gtacgatttc cgaccgtact gagagaacct ttgagcgcct ccgttacctt ttgggaggcg
      421 accgccccag tcaaactgcc cacctgacag tgtcccaaga ccagattcat ggcctatggt
      481 tagagtccca gtactacaag ggtggtatcc caaggatggc tccgccaaaa ctggcgtcct
      541 ggtttcaaag cctcccacct atcctgtaca tgtagtacca agacccaatg tcaagctaca
      601 gtaaagctcc atggggtctt tccgtcctgt cgcaggtatc cggcatcttc accggaatta
      661 caatttcacc gagtctgttg ttgagacagt gcccaaatcg ttacgccttt cgtgcgggtc
      721 ggaacttacc cgacaaggaa tttcgctacc ttaggaccgt tatagttacg gccgccgttt
      781 actgggg
//
LOCUS       DAMJYR010000304          753 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.309, whole genome shotgun sequence.
ACCESSION   DAMJYR010000304 DAMJYR010000000
VERSION     DAMJYR010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 753)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 753)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..753
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.309"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            <1..>753
                     /locus_tag="PC471_003929"
     rRNA            <1..>753
                     /locus_tag="PC471_003929"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          207 a          156 c          227 g          163 t
ORIGIN      
        1 tttaactgag cgttcagtta cttgacgtag acccgaaacc gggcgaccta cccatgagca
       61 ggatgaagcg aaagtaaaat ttcgtggagg tccgaaccca cgagcgttga aaagctcggg
      121 gatgacttgt gggtagcggt gaaattccaa tcgagcccgg agatagctgg ttctccccga
      181 aatagcttta gggctagcct caaggtgaga gatacggagg tagagcactg aatgtcctag
      241 ggggtattgc acctaccgaa gactatcaaa ctccgaatgc cgtcatctta tacttgggag
      301 tcagactgtg ggtgataaga ttcatagtcg aaagggcaac agcccagatc gtcagctaag
      361 gtccctaaat gtaagttaag tggtaaagga tgtgggattg cacagacaac caggatgttg
      421 gcttagaagc agccactcat tcaaagagtg cgtaatagct cactggtcga gtgatcctgc
      481 gccgaagatt tccggggcta aaacttacta ccgaagctac ggcatcagta atgatgggta
      541 ggggagcttc ccatacgggt tgaagcatga ccgtaaggac atgtggacag tatgggagtg
      601 agaatgttgg catgagtagc gagatgtggg tgagaatccc acaggccgta aacccaaggt
      661 ttccagggga aggttcgtcc gccctgggtt agtcgggacc taagctgagg ccgaaaggcg
      721 taggtgatgg acaacaggtt gatattcctg tac
//
LOCUS       DAMJYR010000305          736 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.310, whole genome shotgun sequence.
ACCESSION   DAMJYR010000305 DAMJYR010000000
VERSION     DAMJYR010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 736)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 736)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..736
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.310"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            167..673
                     /locus_tag="PC471_003930"
     CDS             167..673
                     /locus_tag="PC471_003930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase"
                     /protein_id="HDF2790962.1"
                     /translation="MGFREVKIEELQFNPFTKIGKEWLLITAGDSEKFNTMTASWGGV
                     GVYWGKNVVTTYIRPQRYTKEFVDSNDTFTVAFFDETYREALNICGTISGRDINKIEK
                     AGLTPYFVDDTVAFEEANMIIVCKKLYHGNMPPENFDAKENDKKWYPEKDYHTMYISE
                     IIKVLVKE"
BASE COUNT          295 a           74 c          130 g          237 t
ORIGIN      
        1 cacttataaa atatgaaaac tacttattgc gaaagtaatt gatttgttgt attataattt
       61 acatgaatta tgtaggacaa tcaaaagtca gtattataag agtacatata ttcaaatttt
      121 taacaattaa tttatataat taaaaaatga agggtaggat gaagtcatgg gttttagaga
      181 agtaaaaatt gaggaattac agtttaatcc atttactaag attggcaaag aatggttact
      241 tataacagct ggtgattcag aaaagtttaa cacaatgact gctagctggg gtggtgttgg
      301 agtctattgg ggtaaaaatg ttgttacaac ttacataaga ccacagagat atacaaaaga
      361 atttgtagat agtaatgata catttacagt tgccttcttt gatgaaacgt atagagaggc
      421 tttaaatatt tgtggtacta tttctggtag agatataaat aaaatagaaa aagcaggact
      481 tacaccatat tttgtggatg atacagtagc ttttgaagaa gctaatatga ttatagtatg
      541 taaaaagcta tatcatggta atatgccacc agaaaacttt gatgctaaag aaaatgataa
      601 aaaatggtat ccagagaaag actaccatac tatgtacatt tcagaaatta taaaagttct
      661 tgtaaaagaa taattataat aaaaaatata tgaaaagaac ttattataag taaatgactg
      721 tgctttctta tctgat
//
LOCUS       DAMJYR010000306          720 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.311, whole genome shotgun sequence.
ACCESSION   DAMJYR010000306 DAMJYR010000000
VERSION     DAMJYR010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 720)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 720)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..720
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.311"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(344..550)
                     /locus_tag="PC471_003931"
     CDS             complement(344..550)
                     /locus_tag="PC471_003931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2790963.1"
                     /translation="MNQSQEKDLEFIKNHWDLTIIEKERNVTRIIRYSLIILAIIAFF
                     LPTKIKFSIYIFISLFCWLMYLVL"
BASE COUNT          275 a           68 c           90 g          287 t
ORIGIN      
        1 atagtaatta gttttaaatc aatagaatag tatgtattta tctaatgcgg caatataatt
       61 taaagctggt gttgtagaaa aactaagagt gaatatagca aagagaagca atagtatttt
      121 aaactttgtc tctcttattt tttgaaaact aacattaaaa cataaggtat aaataatatt
      181 atcatataaa tgaaaacaaa tttgaatttt tctctggtcg atgtatatag taagtttgtt
      241 gatatgagaa aaattattat tgataatata catgcataga aaggaaatgg tatataatat
      301 tttcttaagt ttctttttta ggcatttcta ttggcatttt aggctatagt actaaataca
      361 ttaaccaaca aaataatgaa ataaatatat atatagaaaa cttaattttc gtgggaagaa
      421 aaaaagcaat aatagcaagt attattagag aataacgaat aatacgagta acatttcttt
      481 ctttttctat gatagttaaa tcccagtgat tttttataaa ttcaaggtct ttttcttgac
      541 tctgattcat ttaatcatct cctgttaata gtataatttt aaagctatta ctttgtttta
      601 ttttaacata tttatacact aaagtgaatt ttatgattag atttatttaa ttatttctga
      661 attaaaactt attagaattc tctattttga taatttcaaa ctctattgat tgtatgataa
//
LOCUS       DAMJYR010000307          712 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.312, whole genome shotgun sequence.
ACCESSION   DAMJYR010000307 DAMJYR010000000
VERSION     DAMJYR010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..712
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.312"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(23..691)
                     /locus_tag="PC471_003932"
     CDS             complement(23..691)
                     /locus_tag="PC471_003932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2790964.1"
                     /translation="MENITSYFTTILCVFICLSSVFIFTQLARVFINKKKINQKIKSR
                     NGFRYDRDFIEARREEIHIKDNNNNKNKSNNKKLKGEKVFKYDNGDLYKGEFVDGKKN
                     GFGIYIFSSKEKYEGLWKDDKMHGIGKYTYRDGSIYTGEFKYGLKNGLGKLTYPNNDI
                     YKGYFLDNKKNGKGVLYKNDGNKQAGIWEDDEQCKSLDFKDLNNNKINNVYQNRLNNN
                     KLKV"
BASE COUNT          227 a          121 c           39 g          325 t
ORIGIN      
        1 aaagattttt ttattagaat aattaaactt ttaatttatt gttatttaat ctattttgat
       61 aaacattatt tattttatta ttatttaaat ctttaaaatc taaactttta cattgttcat
      121 catcttccca tattcctgct tgtttatttc catcattttt atataataca cccttaccat
      181 ttttcttatt atctaaaaaa tatcccttat aaatgtcatt attgggatat gttaattttc
      241 ccaaaccatt ttttaatccg tatttaaatt cacctgtata tattgaacca tctctataag
      301 tatattttcc tattccatgc atcttgtcat ctttccataa tccttcatac ttttccttac
      361 ttgaaaaaat atatatccca aatccatttt tctttccatc aacaaactca cctttatata
      421 aatcaccatt atcgtattta aatacttttt ctccctttaa tttcttattg ttactcttat
      481 ttttattatt attgttatcc tttatatgta tttcttctct tctggcttca ataaaatctc
      541 tatcatacct aaatccgttt cttgatttaa ttttttgatt aatcttcttc ttattaataa
      601 atactctagc tagttgagta aatataaata cagatgataa gcatataaat acacatagta
      661 ttgttgtaaa ataactagtt atattttcca taaaaaatca ccctttaaaa aa
//
LOCUS       DAMJYR010000308          711 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.313, whole genome shotgun sequence.
ACCESSION   DAMJYR010000308 DAMJYR010000000
VERSION     DAMJYR010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 711)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 711)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..711
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.313"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     regulatory      <1..72
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            155..640
                     /locus_tag="PC471_003933"
     CDS             155..640
                     /locus_tag="PC471_003933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HDF2790965.1"
                     /translation="MISELMLKNFRKSSINFKTKIKLNLREKPDINSLKLKSIPEGKI
                     VKLKCVDGIWAEVESNYDKGWLLYKYLERLSNAKNNSNDTIKNRKKNYIGNIRTNGLS
                     LELRNDRTLESKVITTIPDGFKVEVCYSVGKWARVNINKNEKRYSGYVYNQYIEEHLS
                     L"
BASE COUNT          311 a           62 c          117 g          221 t
ORIGIN      
        1 taaatctaat gacgcaaaac tatagggact aaaatcttta tgtaataaag gtcatgtcag
       61 ccagttgcca aaaagataat aattcttttt gttaaaagta tttttagagt tatatatttt
      121 aataacttaa tatttggcaa gaggagggct taagatgata tctgaattaa tgttaaaaaa
      181 ttttcgcaaa tctagcatca attttaagac taaaattaaa ttgaatttaa gagaaaaacc
      241 agacataaat agtttgaaat taaaaagtat accagaaggt aaaattgtta agttaaagtg
      301 tgtagatgga atatgggcag aagttgagag taattatgac aaaggttggt tactttataa
      361 atatttagaa agattaagta atgctaaaaa caatagtaat gatacaataa aaaatagaaa
      421 aaagaattac ataggaaata taagaacaaa tggattaagt ttagaactta gaaatgatag
      481 aacattagaa tctaaagtta taactacaat ccctgatggt tttaaagttg aagtatgtta
      541 tagcgttgga aaatgggcaa gagttaatat caataaaaat gaaaaacgat attctggata
      601 tgtatataat caatacatag aagagcattt aagtttataa tacgatacta gcaagcttta
      661 aatatattaa taaatcgcaa aaaagaatcc cattaatggg attctttttt g
//
LOCUS       DAMJYR010000309          704 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.314, whole genome shotgun sequence.
ACCESSION   DAMJYR010000309 DAMJYR010000000
VERSION     DAMJYR010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 704)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 704)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..704
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.314"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(<1..183)
                     /locus_tag="PC471_003934"
     CDS             complement(<1..183)
                     /locus_tag="PC471_003934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF2790966.1"
                     /translation="MFFSLFKKKSGYTFTNFLSKVRVEKSKKYILKKDLSILDVAVLI
                     GFNSQNYYSIIFKKFNN"
     gene            476..667
                     /locus_tag="PC471_003935"
     CDS             476..667
                     /locus_tag="PC471_003935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2790967.1"
                     /translation="MERLMELIERKFLKDVELLELEDIENVIECKMTQPYNAHYNEYL
                     VKVKNGDEVEEYFVYLKHR"
BASE COUNT          284 a           60 c           90 g          270 t
ORIGIN      
        1 gttattaaac tttttaaaaa ttatactata gtaattttga ctattgaatc caattagtac
       61 agcaacgtct aaaatagaca agtctttttt taatatatac tttttgcttt tttcaactct
      121 aactttactt aaaaaattag taaaagtata tccacttttt ttcttaaata aagaaaaaaa
      181 caacttttat taagttgtaa ttcattacat atattactta ctgttatatc ttctgagtaa
      241 tttttgtgaa tataatctat agttttcttt aagttttctt tacataaaac taagagttgg
      301 tctatgtttc attttatcaa ggcatattta ttaaaaaatc aatctctatt tgttaaaata
      361 atttatgaat gatgttagaa actgtaatat attggatata atataataaa cagataaact
      421 ctgtattaag aggatatata gtttatctta attatagggg aagtgaggat atattatgga
      481 aagattaatg gaactcatag aaagaaaatt tttaaaggat gtagaacttt tagaattaga
      541 agacatagaa aatgtaatag aatgcaaaat gacacagcca tataatgctc attataatga
      601 atatttagta aaagtaaaaa atggagatga agtagaagaa tattttgtgt atttaaaaca
      661 tagataaaca taaaagaggt ttatatttat ttgtatgatt ataa
//
LOCUS       DAMJYR010000310          700 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.315, whole genome shotgun sequence.
ACCESSION   DAMJYR010000310 DAMJYR010000000
VERSION     DAMJYR010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 700)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 700)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..700
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.315"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            101..>700
                     /locus_tag="PC471_003936"
     CDS             101..>700
                     /locus_tag="PC471_003936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HDF2790968.1"
                     /translation="MLDAFITIVSSIIEIWSCKKVFDYTSQSKTDLKKLNMYFLFSVF
                     LIISSLYINIGANSRILICIFVMFVFYKSNYKVNIYKCLVISFFYWLILIGINGLSML
                     LVATVSNLDNMNVLLSGSLYRYEAIFLGKIMLGILLYVYKASFKNFKISKKEIYICIP
                     MIANLLSFFVLYDYIINLVKISPERRTAFLVIVLLLILSN"
BASE COUNT          244 a           56 c           91 g          309 t
ORIGIN      
        1 tatattaaat ttaagtgtgt tttttatgct ataattagta tgtatttttt atgatatatt
       61 ttataaaaaa tataatttaa tcatttttga ggatattatt atgttagatg catttataac
      121 aattgtttct tctattatag aaatatggtc atgtaaaaaa gtttttgatt atactagtca
      181 gtcaaagacc gacttaaaaa aactaaatat gtattttctc ttttctgttt ttctcattat
      241 ttcttcttta tatataaata taggagctaa tagtagaatc ttgatatgta tatttgtaat
      301 gttcgttttc tataagtcta actataaggt taatatatat aaatgtctgg taatctcatt
      361 tttttattgg ttaatattaa taggtataaa tggattaagt atgttattag tagcaacagt
      421 aagcaatttg gataatatga atgttttact tagtggcagt ttatatagat atgaagcaat
      481 attcttggga aagataatgt taggtatatt actttatgtt tataaggctt cttttaaaaa
      541 ttttaaaata agtaaaaaag agatatacat atgtattcct atgatagcaa atttattgtc
      601 attttttgta ctttatgatt atattattaa tcttgtaaaa atctctcctg aaagaagaac
      661 tgctttttta gtaatagttt tactattaat attatctaac
//
LOCUS       DAMJYR010000311          664 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.316, whole genome shotgun sequence.
ACCESSION   DAMJYR010000311 DAMJYR010000000
VERSION     DAMJYR010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..664
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.316"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(<1..>664)
                     /locus_tag="PC471_003937"
     rRNA            complement(<1..>664)
                     /locus_tag="PC471_003937"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          152 a          191 c          151 g          170 t
ORIGIN      
        1 gatccagccg caccttccga tacggctacc ttgttacgac ttcaccccag ttattgattc
       61 caccttcgac gacttcttcc aaaaggttag ataatcggct tcgggcgtct ccaactcccg
      121 tggtgtgacg ggcggtgtgt acaagacccg ggaacgcatt caccgcagca ttctgatctg
      181 cgattactag taactccagc ttcatgtagg cgagtttcag cctacaatcc gaactgagag
      241 tagctttaag ggattagctc caccttacgg cttggcaacc ctctgtacta cccattgtag
      301 cacgtgtgta gccctaagca taaggggcat gatgatttga cgtcatcccc accttcctcc
      361 aggttatccc tggcagtctc tctagagtgc ccaacttaat gatggcaact aaagacaagg
      421 gttgcgctcg ttgcgggact taacccaaca tctcacgaca cgagctgacg acaaccatgc
      481 accacctgtc accaatgtcc ccgaagggaa ctctccgatt aaggagatgt cattgggatg
      541 tcaagcttag gtaaggttct tcgcgttgct tcgaattaaa ccacatgctc cgctacttgt
      601 gcgggtcccc gtcaattcct ttgagtttca ctcttgcgag cgtacttccc aggcggagta
      661 ctta
//
LOCUS       DAMJYR010000312          650 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.317, whole genome shotgun sequence.
ACCESSION   DAMJYR010000312 DAMJYR010000000
VERSION     DAMJYR010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 650)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 650)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..650
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.317"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            231..599
                     /locus_tag="PC471_003938"
     CDS             231..599
                     /locus_tag="PC471_003938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-GCAxxG-C-C family protein"
                     /protein_id="HDF2790969.1"
                     /translation="MTRPSIYHSQGYTCAEALIKSYNEEHNTDIPISIGSGMGVGMNV
                     GSVCGAVNAAVVIIGYLNGRNSNLDENIARKYSRELLNRVRNKYSSEICVSLKKNKVT
                     CEEIINFTYDALNDMLKNQK"
BASE COUNT          285 a           55 c          111 g          199 t
ORIGIN      
        1 tcacagaaaa tagagttaat ttaatatata ataaaagcat atcaagtaaa gctttaaatt
       61 aaagtaactt tataaataaa aagtaatata aattgataaa attataaatt tatttatagt
      121 gaatatgggt tggattttat agatacaaaa atagtatata tgatatgtag accattagca
      181 ggtttaaagt agcaaaacat ataaaataaa atttgaaagg aagtataaaa atgacaagac
      241 catcaatata tcatagccaa ggatacactt gtgcagaggc attaataaag tcttataatg
      301 aggaacataa cacagatata ccaatctcaa taggaagtgg tatgggggtt ggaatgaatg
      361 ttggaagtgt ttgtggagct gtaaatgctg ctgttgttat aatagggtat ttaaatgggc
      421 gaaatagtaa tttagatgaa aatatagcaa ggaagtattc aagagagcta ttgaatagag
      481 taagaaataa atacagctca gaaatatgtg tatctcttaa aaaaaataaa gtaacttgtg
      541 aagaaataat aaatttcaca tatgatgctc taaatgatat gctaaaaaat caaaaataat
      601 tctaagaaac aagtatttta cattcgatag aatacttgtt tttttatgat
//
LOCUS       DAMJYR010000313          638 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.318, whole genome shotgun sequence.
ACCESSION   DAMJYR010000313 DAMJYR010000000
VERSION     DAMJYR010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 638)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 638)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..638
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.318"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            120..410
                     /gene="cas2"
                     /locus_tag="PC471_003939"
     CDS             120..410
                     /gene="cas2"
                     /locus_tag="PC471_003939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas2"
                     /protein_id="HDF2790970.1"
                     /translation="MKKKNNLNYNYVFVVYDVGEKRVSKVFKVCKKYLSHFQKSVFRG
                     EITPSKIMSLKRDLENIIDKDNDFVCIIKLMNSNTFEEEILGNNSNCENMIL"
BASE COUNT          270 a           38 c           95 g          235 t
ORIGIN      
        1 aataatataa gtttttagaa gtattttaag atgagaaagg agaaaatact gaaaaataat
       61 tattctatta taatttgcca aggttttgta tatttttcag tttaaatatt atattacata
      121 tgaaaaagaa aaacaattta aattataatt atgtatttgt agtctatgat gtaggagaaa
      181 aaagagtaag taaagttttt aaagtgtgta aaaaatatct ctcgcatttt caaaaatcag
      241 tttttagagg tgaaataact ccatctaaga ttatgagctt aaaacgtgat ttagaaaata
      301 ttatagataa agataatgat tttgtatgta taataaagtt gatgaattct aatacatttg
      361 aagaagaaat attagggaat aatagtaatt gtgaaaatat gatattataa atctaattac
      421 tatatttgta aaaaataata gcttattaaa gagtagtaaa attgggatat aatttatgat
      481 agagactgca agtagatttt tgtgaaaagg atttacttgc agtatttttg ttatgtataa
      541 ctgttaacat tagtgtacaa aacattatca atagcatttt tctataatga agaaatacta
      601 ttataatggg aaaattttat taatactagg taataata
//
LOCUS       DAMJYR010000314          603 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.319, whole genome shotgun sequence.
ACCESSION   DAMJYR010000314 DAMJYR010000000
VERSION     DAMJYR010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 603)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..603
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.319"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(81..560)
                     /locus_tag="PC471_003940"
     CDS             complement(81..560)
                     /locus_tag="PC471_003940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HDF2790971.1"
                     /translation="MSNKLIKNLLSGILQILFFLLGLVIVAGGFKSFMYLCFSGEATL
                     QGTIYGILMFILGVSYFIIIKSLIEVLGSSEYSLFVKENVKRFRIIGYLLLLNSLIEF
                     ISTFGTTGKGMRFLDLGFGFYFTVPVFVYFITSLMSFVIADGFVKAIKIKEDNDLTI"
BASE COUNT          241 a          101 c           48 g          213 t
ORIGIN      
        1 accctaatat attttatttt aaattactag ttagtcactc ttttcgggtt atttatctcc
       61 tatatattaa agtgtcacat ctaaatagtt aaatcattat cctcttttat ctttattgcc
      121 tttacaaatc catctgcaat tacaaaactc ataagagaag ttataaaata tacaaatact
      181 ggaacagtaa aataaaatcc aaatcctaaa tctagaaatc tcattccttt tccagtagta
      241 ccaaaagttg aaataaactc aattagagag tttaaaagca aaagatatcc aataatccta
      301 aatcttttta cattctcttt tacaaatagg ctatattcag aactacctaa tacttctatc
      361 aatgatttta taatgataaa atatgataca cctaaaataa acattaatat tccataaata
      421 gtaccttgta gagttgcttc tccactaaaa cagaggtaca taaaactttt aaatcctcct
      481 gcaacaatta ctaatcctaa aagaaaaaat aaaatttgta atataccact tagtaaattt
      541 tttataagtt tattagacat catgccaact cctttaaaat attttatata tagtataata
      601 cat
//
LOCUS       DAMJYR010000315          594 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.320, whole genome shotgun sequence.
ACCESSION   DAMJYR010000315 DAMJYR010000000
VERSION     DAMJYR010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 594)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 594)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..594
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.320"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            <1..>594
                     /locus_tag="PC471_003941"
     CDS             <1..>594
                     /locus_tag="PC471_003941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDF2790972.1"
                     /translation="RDKQVPQGRILNATISKEPSGRYYVSLCCTNVDIEVFENTNNQI
                     GLDLGIKEFCISSCGDFIENPKYLKKSLNKLAKLQRELSRKTIGSLNRNKARLKVARL
                     QEHIANQRNDFLQKLSTKLIKENDIICIEDLQVKNMIRNRKLSRLISDVSWSEFVRQL
                     KYKANWHGRQIVKVGKFFASSQICNKCGYKNEEMKDL"
BASE COUNT          249 a           64 c          102 g          179 t
ORIGIN      
        1 taagggataa acaagtacct caaggtagaa tacttaatgc tactatatct aaagaaccaa
       61 gtggtagata ttatgtatca ttatgctgta ctaatgtaga tattgaagta tttgaaaata
      121 ctaataatca gataggttta gatttaggaa ttaaagaatt ttgtatctca agttgtggag
      181 actttataga aaatcctaag tatcttaaga agtcattaaa caaacttgct aaattacaaa
      241 gagaattatc gagaaagaca atcggtagtt taaatagaaa taaagcaaga ttaaaagttg
      301 caagacttca agaacatata gctaatcaaa gaaatgattt tctacaaaaa ctatctacta
      361 aattaataaa agagaatgat attatttgca tagaagattt acaagtaaag aatatgatta
      421 gaaatcgtaa actatctcgc ttaatttctg atgtgtcatg gtcagaattt gttcgtcaat
      481 taaaatacaa ggctaattgg catggtagac aaattgtaaa ggtaggtaaa ttctttgcaa
      541 gttcacaaat atgcaataaa tgtgggtata aaaacgagga aatgaaggac ttaa
//
LOCUS       DAMJYR010000316          577 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.321, whole genome shotgun sequence.
ACCESSION   DAMJYR010000316 DAMJYR010000000
VERSION     DAMJYR010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 577)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 577)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..577
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.321"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            <1..>577
                     /locus_tag="PC471_003942"
     rRNA            <1..>577
                     /locus_tag="PC471_003942"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          154 a          121 c          190 g          112 t
ORIGIN      
        1 caggatggac ccgcgtctga ttagctagtt ggtaaggtaa cggcttacca aggcgacgat
       61 cagtagccga cctgagaggg tgatcggcca cattggaact gagacacggt ccaaactcct
      121 acgggaggca gcagtgggga atattgcaca atgggcgaaa gcctgatgca gcaacgccgc
      181 gtgagtgatg aaggccttcg ggtcgtaaaa ctctgtcctc aaggaagata atgacggtac
      241 ttgaggagga agccccggct aactacgtgc cagcagccgc ggtaatacgt agggggctag
      301 cgttatccgg atttactggg cgtaaagggt gcgtaggcgg tctttcaagt caggagtgaa
      361 aggctacggc tcaaccgtag taagctcttg aaactgggag acttgagtgc aggagaggag
      421 agtggaattc ctagtgtagc ggtgaaatgc gtagatatta ggaggaacac cagttgcgaa
      481 ggcggctctc tggactgtaa ctgacgctga ggcacgaaag cgtggggagc aaacaggatt
      541 agataccctg gtagtccacg ctgtaaacga tgagtac
//
LOCUS       DAMJYR010000317          528 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.322, whole genome shotgun sequence.
ACCESSION   DAMJYR010000317 DAMJYR010000000
VERSION     DAMJYR010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..528
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.322"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(300..500)
                     /locus_tag="PC471_003943"
     CDS             complement(300..500)
                     /locus_tag="PC471_003943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003723180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HDF2790973.1"
                     /translation="MNNGIVKWFNNEKGFGFISMESGDDVFAHFSAIQTSGFKSLEEG
                     QKVSFDIVKGARGPQAENITIL"
BASE COUNT          192 a           68 c           54 g          214 t
ORIGIN      
        1 tactaaaaat gttatatcag aattgaatac aaaagaaaga gtatgtattt atgagatagt
       61 tgaaaatttt gatgtgccac aactaaaatt attatattac tatatattta attaaaagag
      121 tacttgtagg tttaagtaca tactttataa atatattttt gttagattta ttttataaaa
      181 agtatttaga ctaagtatta tttttaagta ttttcttgtt gaagttattt tttccttttt
      241 tacaaaattc gtaaataaaa aaaagactct taaaagagtc tttccgaaaa atctatatat
      301 tataatatag ttatattttc tgcttgagga cctctagcgc ctttaactat atcaaagctt
      361 actttttgac cttcttctaa tgatttaaat cctgaagttt gtatagctga gaaatgagca
      421 aatacgtcat ctccactttc cattgatata aatccaaatc ctttttcatt attaaaccat
      481 tttactattc cattattcat taaaaatact cctattaacc tatatcaa
//
LOCUS       DAMJYR010000318          505 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.323, whole genome shotgun sequence.
ACCESSION   DAMJYR010000318 DAMJYR010000000
VERSION     DAMJYR010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 505)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 505)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..505
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.323"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            105..326
                     /locus_tag="PC471_003944"
     CDS             105..326
                     /locus_tag="PC471_003944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2790974.1"
                     /translation="MDRLKRDMKNMKEDLKEGVKKTAYVMKNTAEDLKDDAQGAMVNL
                     EMKKDEMMERYEQKKFSKELEKQMKNEMK"
BASE COUNT          234 a           28 c           87 g          156 t
ORIGIN      
        1 ctatatttaa gacttaaaaa ttataattga tattacaaat aaatatatgt actttgggaa
       61 aaataaatat aggaagtatt atatttacag aggaggtagt tttgatggat agattaaaaa
      121 gagatatgaa aaatatgaaa gaagatttaa aagaaggtgt taaaaagaca gcttatgtta
      181 tgaaaaatac agctgaagat ttgaaagatg atgcacaagg tgctatggta aacttagaaa
      241 tgaaaaaaga tgagatgatg gaaagatatg aacaaaagaa attttctaaa gaattagaga
      301 agcaaatgaa gaatgaaatg aaataagctt ttatttatga ataacaaatt attgtattca
      361 tataaaaagt agccagtaat attaaatagt ttatttaagg aaatagactt tctaaaaata
      421 actgtattag atttagaaag tctatttcaa attagtttta taaatagaca taaggataaa
      481 gtagatgtta tagaactaga tttta
//
LOCUS       DAMJYR010000319          496 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.324, whole genome shotgun sequence.
ACCESSION   DAMJYR010000319 DAMJYR010000000
VERSION     DAMJYR010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 496)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 496)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..496
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.324"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            <1..>496
                     /gene="tuf"
                     /locus_tag="PC471_003945"
     CDS             <1..>496
                     /gene="tuf"
                     /locus_tag="PC471_003945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887863.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HDF2790975.1"
                     /translation="GTVATGRVERGVLKVQDEVELVGLTEAPRKVVVTGVEMFRKLLD
                     QAQAGDNIGALLRGVQRNEIERGQVLAKTGSVKAHTKFTAEVYVLKKEEGGRHTPFFD
                     GYRPQFYFRTTDVTGACKLPEGIEMVMPGDNVTMEVDLINSIVVEEGLRFSIREGGRT
                     VASGV"
BASE COUNT          213 a           61 c          125 g           97 t
ORIGIN      
        1 ggaacagttg caacaggaag agtggaaaga ggagtactaa aagtacaaga cgaagtagaa
       61 ttagtaggat taacagaagc accaagaaaa gtagtagtaa caggagtaga gatgttcaga
      121 aaattattag accaagcaca agcaggggat aatataggag cattattaag aggagtacaa
      181 agaaacgaga tagaaagagg acaagtacta gcaaagactg gatcagtaaa ggcacacaca
      241 aagtttacag cagaagtata tgtacttaaa aaagaagaag gtggaagaca tacaccattc
      301 tttgatggat atagaccaca attctatttc agaacaacag acgtaacagg agcttgtaag
      361 ttaccagaag gaatagagat ggtaatgcct ggagataacg taacaatgga agtagactta
      421 ataaactcaa tagttgtaga agagggatta agattctcaa taagagaagg tggaagaaca
      481 gtagcttcag gagttg
//
LOCUS       DAMJYR010000320          493 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.325, whole genome shotgun sequence.
ACCESSION   DAMJYR010000320 DAMJYR010000000
VERSION     DAMJYR010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..493
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.325"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            188..304
                     /gene="rrf"
                     /locus_tag="PC471_003946"
     rRNA            188..304
                     /gene="rrf"
                     /locus_tag="PC471_003946"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            310..398
                     /locus_tag="PC471_003947"
     tRNA            310..398
                     /locus_tag="PC471_003947"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:344..346,aa:Leu,seq:taa)
     gene            410..486
                     /locus_tag="PC471_003948"
     tRNA            410..486
                     /locus_tag="PC471_003948"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:444..446,aa:Ile,seq:cat)
BASE COUNT          156 a           91 c          109 g          137 t
ORIGIN      
        1 attcattaag atatatgtgc agtttttaga gtgctaactc tatattttta aacttgtcaa
       61 aacttatgaa tttttaatag ttttataagg caaaaaatcg aataaaaaaa gtattgacac
      121 aataagacaa agatgttatt ataatacttg tcttagaaaa aaagttaaat gaaaatttag
      181 acaataatct ggttataata gcagagagga tacacctgtt cccattccga acacagaagt
      241 taagctctct agcgctgatg gtacttggtg ggaaactgcc tgggagagta ggacatagcc
      301 aggtgatgtg ccgaagtggc ggaactggca gacgcacagg acttaaaatc ctgcgggact
      361 tacctctcgt accggttcga ttccggtctt cggcaccaca aattattgtg ggccattagc
      421 tcagttggtt agagcgcccg gctcataacc ggtaggtctg gggttcgagt ccctgatggc
      481 ccaccacaat aat
//
LOCUS       DAMJYR010000321          411 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.326, whole genome shotgun sequence.
ACCESSION   DAMJYR010000321 DAMJYR010000000
VERSION     DAMJYR010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 411)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 411)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..411
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.326"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            67..>411
                     /locus_tag="PC471_003949"
     CDS             67..>411
                     /locus_tag="PC471_003949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2790976.1"
                     /translation="MNKKKKLIGIAISFLLIISIINPLQVEAEAIIESSVYISSIKEN
                     YTYDNTKFKTIKSKSRHKSRKYNKSKVKLKKRSIRTKKYGYQTINKYKRQNARRHNIL
                     TKLILSIIVIFLV"
BASE COUNT          192 a           35 c           54 g          130 t
ORIGIN      
        1 atttgtaatt tttagttata tatatattat aatgaactag ttaaaaattt ttatgagggg
       61 gaaaatatga ataagaagaa aaaattgatt gggatagcta tatcttttct acttataatc
      121 agcattataa atccattaca agtagaagca gaagctatta ttgagtctag tgtatacatt
      181 tcttcaataa aagagaatta tacatatgat aatactaaat ttaaaactat taaatctaaa
      241 agtagacata aaagtaggaa atataataaa agtaaagtaa agttgaaaaa acgaagtata
      301 agaactaaaa agtatggata tcaaactata aataaatata aaagacaaaa cgcaagaaga
      361 cataatatcc taacaaaatt aatattgtcc ataatagtca tatttttggt t
//
LOCUS       DAMJYR010000322          398 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.327, whole genome shotgun sequence.
ACCESSION   DAMJYR010000322 DAMJYR010000000
VERSION     DAMJYR010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..398
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.327"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            50..331
                     /locus_tag="PC471_003950"
     CDS             50..331
                     /locus_tag="PC471_003950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2790977.1"
                     /translation="MASKQIPRNNLEKFLRIRVLSHTGDKIHIKLPVNFAKRMIENNA
                     LDLFNGKDDVVDGKKVTEILVKAFDYDLTGEVVHLERKNGDIIKVSIVQ"
BASE COUNT          176 a           26 c           66 g          130 t
ORIGIN      
        1 gaataaaatt tattattaaa taaaaaataa aggagagtga tttaatagca tggcatcaaa
       61 acaaatacca agaaacaatt tagaaaagtt tttaagaata agagtcttat cacatacagg
      121 cgataaaata catataaagt tacctgtaaa ttttgcaaag agaatgattg agaataatgc
      181 tttggattta tttaatggaa aagatgatgt agtagatggt aaaaaagtta ctgaaatatt
      241 agttaaggca tttgactatg atttaactgg agaagtagtt cacttggaga gaaaaaatgg
      301 agacattatt aaagtttcaa ttgtacaata gaattatttt atatgaattt tatttttgaa
      361 tagttataaa taattagaaa tttgtaaaaa agtataat
//
LOCUS       DAMJYR010000323          390 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.328, whole genome shotgun sequence.
ACCESSION   DAMJYR010000323 DAMJYR010000000
VERSION     DAMJYR010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..390
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.328"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
BASE COUNT          154 a           30 c           48 g          158 t
ORIGIN      
        1 aatgtataag gtggtaaatt tatataaaaa agaatatgag ttgtaaaata ttaaataata
       61 ttgtactaat tggaataatt ttaactttat tatagattat tttaatacca ttagtactga
      121 tggtgtattt taaaattggt ataagcatta caaatacgaa tatgccaact gtaatatgta
      181 atataagtag ctatatacat atgtgctgtc ccatatattg tatcattgtt tatattaaaa
      241 agattatgta aattggtatc aatcaataaa tcattttcag aagaaatacc taaaaactta
      301 aaaaatacct atatgtgtat ttagttagat ttgagatttt tacatttaat tttatagtga
      361 caatattata ttatttttat gatgtctatt
//
LOCUS       DAMJYR010000324          374 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.329, whole genome shotgun sequence.
ACCESSION   DAMJYR010000324 DAMJYR010000000
VERSION     DAMJYR010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..374
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.329"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(<1..372)
                     /locus_tag="PC471_003951"
     rRNA            complement(<1..372)
                     /locus_tag="PC471_003951"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT           87 a          102 c           63 g          122 t
ORIGIN      
        1 aggactatta ccttctgtgg tgggtctttc caaacctctt caattacgat acctcttcgt
       61 tatgatatgt ccgcaacccc aataaagaaa actttattgg tttgggctaa tccgcgttcg
      121 ctcgccgcta cttacggaat cgaatttctt tctcttcctt caggtactta gatgtttcag
      181 ttccctgagt tcccctcatt aagctatgta ttcacttaat gatacttaga cattactcta
      241 agtgagtttc ctcattcgga aatcttcgga tcaaagttta cgtgcaactc cccgaagctt
      301 atcgcagctt atcacgtcct tcatcggctc ctagtgccaa ggcatccgcc ctgcaccctt
      361 aataacttga ccag
//
LOCUS       DAMJYR010000325          364 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.330, whole genome shotgun sequence.
ACCESSION   DAMJYR010000325 DAMJYR010000000
VERSION     DAMJYR010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..364
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.330"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            168..284
                     /gene="rrf"
                     /locus_tag="PC471_003952"
     rRNA            168..284
                     /gene="rrf"
                     /locus_tag="PC471_003952"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            291..362
                     /locus_tag="PC471_003953"
     tRNA            291..362
                     /locus_tag="PC471_003953"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:323..325,aa:Asn,seq:gtt)
BASE COUNT          117 a           61 c           78 g          108 t
ORIGIN      
        1 attcattaag atatatgtgc agtttttaga gtgttaactc tatgtaaagt actaacttca
       61 attgatgaag tatatatcgt aaaaacaatt actatttagt acttcaaaac tattgacatt
      121 taaaaataaa gatgttaata taatacttgt cttataaata ggcaatatgt ggttataata
      181 gcaaagagga tacacctgtt cccattccga acacagaagt taagctcttt agcgctgatg
      241 gtacttggtg ggaaactgcc tgggagagta ggacgtagcc acgtgaatat tccaaggtag
      301 ctcaatggtg gagcaaccgg ctgttaaccg gtaggctgtg ggttcgagcc ccacccttgg
      361 agcc
//
LOCUS       DAMJYR010000326          339 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.331, whole genome shotgun sequence.
ACCESSION   DAMJYR010000326 DAMJYR010000000
VERSION     DAMJYR010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 339)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..339
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.331"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
BASE COUNT          131 a           34 c           29 g          145 t
ORIGIN      
        1 agtataattt tattgattct acctttttaa ataactgtcg atttattaaa gttatctatg
       61 tactatatta aacaattaag tagttattaa ttgaacttat tagtttatta aaaaactatg
      121 tatattactt tgcagtaaga ctttggaact aaatctataa tagcaaattt aaatttatta
      181 aatagattac cttataacat ttcatataaa atatattgtg aatctgagaa aattactatt
      241 tttctttgag tattttttgt aagcagttct aaaagattca atttcatacc tattacttga
      301 atcattcatt aaaataattc taatttaaaa aataattct
//
LOCUS       DAMJYR010000327          317 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.332, whole genome shotgun sequence.
ACCESSION   DAMJYR010000327 DAMJYR010000000
VERSION     DAMJYR010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 317)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 317)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..317
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.332"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
BASE COUNT          107 a           33 c           31 g          146 t
ORIGIN      
        1 atttatataa atattttttg tttttaattt tttctatctt cttatgtact tacatttaat
       61 ttattctttt cagttaataa aataggtaca tttttagtac atgaaaatag agttgcttac
      121 agggtatata ggattgatgt actatttact aatattacat tttcaactga ttcaacccct
      181 cttttactta cataatttaa gtatattttt ctggattttt aaatttatgt actttaaaat
      241 ggatatactt attttcattg attttgtaag aattaataaa aaatacgatt acactacaaa
      301 attagaatgt caatgtt
//
LOCUS       DAMJYR010000328          310 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.333, whole genome shotgun sequence.
ACCESSION   DAMJYR010000328 DAMJYR010000000
VERSION     DAMJYR010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 310)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 310)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..310
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.333"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
BASE COUNT          130 a           24 c           42 g          114 t
ORIGIN      
        1 aaagatatta cattaatatg aaaatatagg agattagaag tgtttgataa ttacagtaca
       61 tgttagatat atcattgtaa attaacatag ctttgaatat aataatgttc agaaggcaat
      121 atttttgagg tctgaatcca ataataatat agtcttatga ttgtagatat caaaaaatgt
      181 aagttactag gaatacgttt tgtagattta tatatgatta ataactaaag ctataaataa
      241 acaaaataaa aagagtatgt ttcaacctat agcttttaga tttaaaatat actcttttat
      301 taattatagt
//
LOCUS       DAMJYR010000329          270 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.334, whole genome shotgun sequence.
ACCESSION   DAMJYR010000329 DAMJYR010000000
VERSION     DAMJYR010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 270)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 270)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..270
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.334"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
BASE COUNT          135 a           27 c           29 g           79 t
ORIGIN      
        1 ctaaacttat agattaaaaa atgacttatt tatagatgaa aaaccaaact catagattaa
       61 aaaataaatg acttatagat gaaaaatcaa gctcatagat taaagaataa ataacttatt
      121 agatgaaaaa ccaagcttat agataagaag tccatgctta taagccagaa aatgaatact
      181 ttaatagatt aaaaaataca tatttgtgaa ttatagaatg aatgactcca taaactagaa
      241 aatttataat aatatctata taaaaattag
//
LOCUS       DAMJYR010000330          232 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.denovo.336, whole genome shotgun sequence.
ACCESSION   DAMJYR010000330 DAMJYR010000000
VERSION     DAMJYR010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 232)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 232)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..232
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.denovo.336"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(<1..>232)
                     /locus_tag="PC471_003954"
     CDS             complement(<1..>232)
                     /locus_tag="PC471_003954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002590471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptidase dimerization domain-containing
                     protein"
                     /protein_id="HDF2790978.1"
                     /translation="NVIQKIQQLVPPTHPVLGDGILVLTDIKSSPYPGASVVPDYCKA
                     TFDRRLLVGETREGVLAPIQALLDEMMKEDPE"
BASE COUNT           66 a           41 c           37 g           88 t
ORIGIN      
        1 aattctgggt cttctttcat catttcatct aataaagctt gtattggagc taatacgcct
       61 tctcttgttt ctccaactaa taatcttcta tcaaaagtag ctttgcaata atctggcact
      121 actgatgctc ctggatatgg agaagatttt atatctgtta aaactaatat tccatcacct
      181 aaaactggat gagttggagg tactaattgt tgtatctttt ggattacatt ag
//
LOCUS       DAMJYR010000331        17286 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.guided.081, whole genome shotgun sequence.
ACCESSION   DAMJYR010000331 DAMJYR010000000
VERSION     DAMJYR010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 17286)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17286)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17286
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.guided.081"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(347..871)
                     /locus_tag="PC471_003955"
     CDS             complement(347..871)
                     /locus_tag="PC471_003955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HDF2790979.1"
                     /translation="MENKNPIVTIEMENGKEIKIELYPNIAPNTVKNFVSLVNEGYYN
                     GIIFHRVIPGFMIQGGCPNGTGMGGPGHSIKGEFSGNGFTNNLKHERGVISMARTMAP
                     NSAGSQFFIMHKNSPHLDGQYAGFGRVIEGMDTVDEIASVRTDSADKPQTPQVMKSVT
                     VETFGIDYSDVEKN"
     gene            complement(936..1688)
                     /locus_tag="PC471_003956"
     CDS             complement(936..1688)
                     /locus_tag="PC471_003956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HDF2790980.1"
                     /translation="MKLGLCLEGGGAKGAYQAGVVKALYDGGINKFYSISGTSIGAIN
                     GYYLYTGNVDNLEKMWTNIKDIQNGNVKIVNNTVDNSPAIDNLRELDDSNIEEMNFYV
                     NYVEVDNKVVSEKIVDVSKMPRNEAIISISYSGLLPSNPNATLGFKEQFVKDVQEGIY
                     DGFKLDGGLIRSALIEPLIGDNVDKIILISTKYNYELPEDIKKVYDEDKIIVVRPNTQ
                     FAPKDTLNFDDEFCKTIYQEGYEIGKNILDRL"
     gene            complement(1719..3017)
                     /locus_tag="PC471_003957"
     CDS             complement(1719..3017)
                     /locus_tag="PC471_003957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M18 family aminopeptidase"
                     /protein_id="HDF2790981.1"
                     /translation="MDSINFAKNLIDFIYDSPSSFHAVESTKEILDKNGFEELVLNQR
                     WNLRVGGKYYVTKNLSAIVAFVVNSEGIERDGFRIIGSHSDSPTFSIKPNAEIEAEKS
                     YLKLNTECYGGAILSTWLDRPLGIAGRVVLKGDSILKPNERLIDFKKPICIIPNLAIH
                     LNRSINDGHSYNKQKDMLPLVGMLNDTLEKDNFLIKQIASNLDVNMDDIIDFDLFLYE
                     FEKGSLIGANDEFISIGRQDNLAMVHASLNALVNTKGQHGVNVMAVFDNEEVGSSTKQ
                     GADSNMLLNILERICISMGKDREGFFESIYSSFMISSDLAHAIHPNIPEKHDPTNKPI
                     MGKGPVIKINASQAYTSDGYSIAIYKNICREANVEYQEFVNRSDERGGSTIGPISSTH
                     IDIPSVDVGSPILAMHSIRELGNVEDHYSIYKTFSKFFEI"
     gene            complement(3034..5298)
                     /locus_tag="PC471_003958"
     CDS             complement(3034..5298)
                     /locus_tag="PC471_003958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3553 domain-containing protein"
                     /protein_id="HDF2790982.1"
                     /translation="MNLDTLNPAQREAVEKTEGPVLILAGAGSGKTKVLTTRIAYLIE
                     DKGVQAPNILAITFTNKAANEMRERVEQNIGPETKDMWISTFHSCCVRILRKDINKIG
                     YNRSFVIYDSADQVTLVKDCLKELNLSDKVFEPKAVISAISGAKDKLYTPKQFKDINM
                     ADNRMVKIADIYALYQDRLKRNSALDFDDLILKTVELFKANDEVLAYYRSRFRYIMVD
                     EYQDTSKAQYELIKLLAREHQNICVVGDDDQSIYGWRGADIRNILEFEKDYDNVHVVK
                     LEQNYRSTQVILDAANKVISNNIERKRKKLWSEKKEGELIKIQLTGSEIEEADFIADS
                     IAQIARKENRPYKDFAVLYRANAQARPVEDALNRSQIPYNIYGGTKFYERKEIKDLLA
                     YLRVIQNPQDDISIKRIINVPRRGIGLRTIEKIEDRANLKQESIYSVLIDIETNSDIS
                     TKARASISGFVDIIGTLRTIKEVYPVSKLIEKVLDTTGYMDELVEIRNKNEKDLTGKG
                     EEAQDRIDNLREFISIALEFESSNDDTYENKDLETFLTSIALTSESNDEEDNDRVSLM
                     TIHTSKGLEFPVVFLTGMEEGLFPISRAIKSMSDSQIEEERRLCYVGITRAKEELYMS
                     LTEKRTLYGKTNVAIASRFMEELPEECIERLYKVKKELSYSKASYNMLDKYTKKYMST
                     ISKSKVADKVNATIKDSNKETNPDDIKLGSKVHHPKFGVGTVVSIIGTDVTIAFDQQG
                     IKKINKEYTTLNIL"
     gene            complement(5553..6371)
                     /locus_tag="PC471_003959"
     CDS             complement(5553..6371)
                     /locus_tag="PC471_003959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HDF2790983.1"
                     /translation="MFKINNLTYQYEKNTNALLNINMDFSKGNVIGIIGSNGSGKSTL
                     FMNLMGILKPTSGEILFKEEKLKYDKRSLYNLRKNVGIVFQDPEKQIFYSKVYDDIAF
                     AMRNIGMDEKTIKERINKALVAVNGIDFIDRPVHFLSYGQKKRVAIASVIAMENEIVL
                     LDEPTAGLDPVSTRSIVDIIKGLNKNNIKIVISSHDMNLMYEICDYIYVLDKGILIDE
                     GKAENVFINENNIIQAGLESPWLVKVHRNMNLPLFKKEEDLYKYWKERELNTNK"
     gene            complement(6364..7062)
                     /gene="cbiQ"
                     /locus_tag="PC471_003960"
     CDS             complement(6364..7062)
                     /gene="cbiQ"
                     /locus_tag="PC471_003960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt ECF transporter T component CbiQ"
                     /protein_id="HDF2790984.1"
                     /translation="MLIIDKYAYTNRLSKVNPNKKVAIGVIFLIASMVIQNIFILSGI
                     MILMSILVVCVAGIDLKNYLKLLRIPMYFLFLSIGITLVNISFNKADLLYSFEFFSFN
                     VGISKASINMSIHVLFRAMSCLTCVYFCILTTPFNQLIFFFKKLHLPDTFVELSMLIY
                     RFIFIFLEEFSEIYKSQELRFGYINLKTSYKSLGILGSMLYKRLMTRYDDMCISLDIK
                     LYDGKFHIVGDNDV"
     gene            complement(7065..7349)
                     /locus_tag="PC471_003961"
     CDS             complement(7065..7349)
                     /locus_tag="PC471_003961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     substrate-binding protein"
                     /protein_id="HDF2790985.1"
                     /translation="MSAKTKTKTNILLLLLVVALIIFPLLVNSGAEYGGADGQAESEI
                     TKINPNYKPWFSSPYEPPSGEIESLLFSAQAALGAGVIGYILGLQKGKRS"
     gene            complement(7339..8091)
                     /locus_tag="PC471_003962"
     CDS             complement(7339..8091)
                     /locus_tag="PC471_003962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006440549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter permease"
                     /protein_id="HDF2790986.1"
                     /translation="MKQNIKLGVIAALMLIILTPVTSNAMHIMEGYLPVKWSIAWGVI
                     FIPFFLFGLKSIGKIVKQDPKKKVLLALCGAFVFVLSALKIPSVTGSCSHPTGVGLGA
                     IMFGPSVMFVLGTIVLIFQALLLAHGGITTLGANAFSMAIIGPIVSFLIFKALKKKDG
                     NNAIPVFLAAAIGDLATYTITSIQLALAFPDPSGGVMASAIKFLGIFFMTQIPIAIAE
                     GILTVIVYNLITENGEKSILENNDKGVKANEC"
     regulatory      complement(8273..8465)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            complement(8597..10369)
                     /locus_tag="PC471_003963"
     CDS             complement(8597..10369)
                     /locus_tag="PC471_003963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase activation-like protein"
                     /protein_id="HDF2790987.1"
                     /translation="MKWIYDDYEFENRANNLSWTIAGDYNESIDISEKDYSSKEIGLY
                     FAIIAGARRKYVDWDIVKKYLTNRIRKGYNKDVILGLIQVILNTIVEEKVIKDRPGIE
                     DIRRNAYKDIVSRFTKIHNDNIMDKIKYTFVLYSMDKHPALDSMTRRIVNDIRNIDTN
                     QDIMEILKALDVIYLTYFEYILNGEENSLIDENNVESVEVNFDTFADFMYEELYSDEE
                     ENIIESDIDSISSSMLVEGVGDFDDSKCSNSADRVIYVDEDTANKIYSKIEHYYGKTY
                     LSKGEIKKIETQNCRNVHEGCRIHFTDGVLRSECNNVFQLKYVTRQKENNLGKFREHA
                     RMYRQQIKRLKESLSRILIEENAKSRVYSDCGTILANRAWRVGRSNNNKIFYKDIENE
                     KGKYVIDILLDASGSQSRNQGNVAIQAYIIANALTEVGIPNRVMGFSSFLDYTILKRF
                     KDYDDSIKNSENIFEYFCAGNNRDGLAIKSICSGLINRDEENKILIVLSDGRPNDVKI
                     GKSSERTLRGEKAYRGIVGLRDTANEVRKARKQNILVLGVFTGKESELEAERLIYGND
                     FIYSRDITRFSDVVSMYLKKIIRN"
     gene            complement(10383..11312)
                     /locus_tag="PC471_003964"
     CDS             complement(10383..11312)
                     /locus_tag="PC471_003964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDF2790988.1"
                     /translation="MDIIDNLRVQGVDEKLIEDVLYFRNYYGLEKDLEYRVTKSKTYF
                     YGKDILSMCIAAILEEENILLSGPKATGKNLLADNLGEIFNRPQWNTSFHINTDSSTL
                     IGTDTFIDNEVKLRRGSVYECAINGGFGVFDEINMAKNDAIVVVHSALDYRRIIDVPG
                     YERVNLHPATRFIGTMNYEYAGTKELNEALVSRFMVIDIPPIEEDKLMMILKNEFSDA
                     DEEKLIHFAGIFLDLQLKSQNGEISSKAIDLRGLMASLKTIRRGLKPTLAINMGLTGK
                     TFDVYEKEMVGDVIKTRIPNKWESIDVFPISHI"
     gene            11533..11718
                     /locus_tag="PC471_003965"
     CDS             11533..11718
                     /locus_tag="PC471_003965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HDF2790989.1"
                     /translation="MKYGCPEVAKKWGISERRVQKLCEESRIPGVSKLGYMWLIPKNA
                     EKPVDGRTKRRKEVQYE"
     gene            11711..12412
                     /locus_tag="PC471_003966"
     CDS             11711..12412
                     /locus_tag="PC471_003966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2790990.1"
                     /translation="MNSVLIIDDDKELCALMKKCVEQENLSAITAHCGAEGLHLVDKN
                     RHTLSLVILDIMMPDIDGFQVLKKIRETSNIPVIMLTAKNDEDDKVSGLRLGADDYLT
                     KPFSINELMARVNSLIRRYTMLNPAFITDTDCIIINGMIIDKTNRIVSVNNIPVQLTS
                     KEFDLLAFLASNKGRVFTKKQIYTQVWEEEYAFDDSNIMSFISKLRKKIEPDPNHPFY
                     ILTVRGVGYRFNKEA"
     gene            12414..13337
                     /locus_tag="PC471_003967"
     CDS             12414..13337
                     /locus_tag="PC471_003967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF2790991.1"
                     /translation="MNTSVYLLLILVLSLIIIAYLVSVLLRVRIQLTLIKDALEDIKN
                     GNLNRRILTDENDITKQICYDINEIAINSQFQLIQQKQSEQAYKQLMTSLSHDVKTPL
                     ASLVGYLEAVESKIVVGEEKDEYIHVASNKAHYLKNFVENLFEWVKLDSKEQVFHFDI
                     FDLNELSRNIISDWIPVLESSHFEYEFDIPEIEYFLRIDANAYTRIINNLLQNIITHS
                     SGNKMTLRIFENKEQAQIIITDNGKGISSDNLPHIFERLYQCDHSRASKGNGLGLAIA
                     KELINAHKGNITANSTPGMGTEFTILLPKAL"
     gene            13494..14417
                     /locus_tag="PC471_003968"
     CDS             13494..14417
                     /locus_tag="PC471_003968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019639192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2790992.1"
                     /translation="MNDFVIETKQLTKIYGNQTVVDKVNLHAKKGRIYGLLGRNGAGK
                     TTIMKMILGLTSITYGEVDVFGENIKGREKQVYPRIGAIIETPGFYPNLTGTENLEIF
                     AKLRGTAAPNSVKRALEIVGLPYKNKKLFSEYSLGMKQRLGIANAILHDPELLVLDEP
                     TNGLDPIGIAEMRKFIRDLSVEHGKTILISSHILSEISLLADDIGIIDHGVLLEESSM
                     SELEKKNSKYILLQVSDIPKTSLILERQFNTKDYSVQDDYTLHLYNTNLDMGEINKAL
                     VMQNVTVISSQLCNDTLEDYFKKITGGEGIA"
     gene            14410..15147
                     /locus_tag="PC471_003969"
     CDS             14410..15147
                     /locus_tag="PC471_003969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2790993.1"
                     /translation="MLKLIQTEFLKLRRRKLIWLMLLSALIMPFFALLYFNYFGKTGV
                     EPIKFYKWSAFGYTLWIILPVVLGIFCTMLIHEENQYDMLKQLWIVPISKMGYFFSKF
                     FVVLIYSICFMLITAVASVLFSVLSGYVVFSWASILYLLKKCLEIGVITAFVMLPILA
                     IATSQKGYILPICITLVYAFSGFVIMTINMYLHPLSSMGVIIMRNKDIPGLIFSQEIN
                     VPLAFLCICIWSIVSVLFANTALGRRK"
     gene            15158..15931
                     /locus_tag="PC471_003970"
     CDS             15158..15931
                     /locus_tag="PC471_003970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2790994.1"
                     /translation="MWKLLWAECQKIRRSKIVWITVFASIMIAVIVFAGGQDVYNDPD
                     LHYGLKSVYDGSRYIDNAGWYMDAVQPWSTFFVLPAVIALLGSYIICREEEEDTIKSL
                     RLIPINETKLTIAKMIITFIFSILLYLLLFTITFLTESVLHFSDLSTKLVLSCMKEYF
                     LDGIGVFFAISPIIALVSRMKKGYWLALVFTEVYSIAGLFAGMSNVLQTFFPIAAIFN
                     ISGYYITTGEKIMGSIISLLLCACLSAFILKGLKHNEKN"
     gene            complement(16472..17080)
                     /locus_tag="PC471_003971"
     CDS             complement(16472..17080)
                     /locus_tag="PC471_003971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HDF2790995.1"
                     /translation="MSVINSFILQYGLISVFVLIMIEYACFPLPSEVVLPLCGAIAAR
                     NHFGFLTILILSIIAGILGSIFCYTVGNWGGKSIINKIIEICPKAKKGIFASQDYFNK
                     YSSISVCVCRLIPLCRTYISFIAGIAGQNIITFVISSIVGITIWNTSLISIGYIFSES
                     WVRIMSHYNDYKFLVLIIPISIIFIGFVIKSFIHKKHHKIRL"
BASE COUNT         5600 a         2978 c         2170 g         6538 t
ORIGIN      
        1 aatatctttt tataagtaaa aattataaaa aaccttattt ttattaaata gaataaataa
       61 aataagttta aaaaatactg aaataagtgt tttccacata ttttatatat ttatttaata
      121 cattattatt taaaaataaa tatataaaca atcataataa aattttttta ataaaacttt
      181 attttttagt taaaatcata caatatatag atttccacaa tacaaataaa ttaaaaagtg
      241 aaatgtattt gtctaaaaac ataataacat ttcactttat cctcattttt aagtaatata
      301 tatttttaat ttttatttat aactaaatct agtttttata taaattttag tttttttcta
      361 catctgagta atctattcca aaagtttcta cagtaacaga tttcataact tgtggagttt
      421 gtggcttatc agctgaatct gttctaactg atgctatttc atcaactgta tccatacctt
      481 ctatcactct accaaaacct gcatactgac catctaagtg tggtgagttt ttatgcatta
      541 tgaaaaattg agaaccagct gaattaggtg ccatagttct agccattgat atgactcctc
      601 tttcatgttt taaattgtta gtaaatccat taccactaaa ttcacctttt attgagtgac
      661 ctggaccacc cattcctgta ccatttgggc atcctccttg tatcataaat cctggtatta
      721 ctctatgaaa tattattcca ttgtaatatc cttcatttac taatgataca aagtttttaa
      781 ctgtatttgg agctatattt ggatataact caattttgat ttcttttcca ttttccattt
      841 ctatagttac tataggattt ttattttcca taatttatta ccttcctttt cattagttaa
      901 attacataat tacatttatt atattatact ctaaattaaa gtctatcaag tatatttttt
      961 cctatttcat atccttcttg ataaatagtt ttacaaaact catcatcaaa gttaagcgtg
     1021 tctttaggag caaattgagt attaggtcta actactatta ttttatcttc atcatatact
     1081 tttttaatat cttctggcaa ctcataatta tattttgtgg atattaaaat aattttatcc
     1141 acattatccc caattaatgg ttcaattaat gcactcctaa ttaaacctcc atctaatttg
     1201 aaaccatcat aaattccttc ttgcacatct ttcacaaatt gttctttaaa tccaagagtt
     1261 gcgtttggat ttgatggtaa caacccactg tatgaaatgc tgataatggc ttcattacga
     1321 ggcatttttg atacgtcaac aatcttttct gatactactt tattatcaac ttcaacataa
     1381 ttaacataaa agttcatttc ttctatatta ctatcatcta gttcccttag gttgtctata
     1441 gcaggagaat tatccacagt attatttact atttttacat ttccattctg gatatctttt
     1501 atatttgtcc acattttctc caagttatca acattccctg tgtacaaata atatccattt
     1561 attgctccta ttgatgtccc tgaaatcgaa taaaatttat ttattcctcc atcatacaag
     1621 gctttaacta ctcctgcttg ataagcccct tttgctcctc caccttctaa acataatcct
     1681 aatttcatag tctcatctcc aaattgttta tcttatattt atatttcaaa aaacttacta
     1741 aatgttttat atatactata gtggtcctca acatttccta attctcttat agaatgcatt
     1801 gctaaaattg gacttcccac atccacagat ggtatatcta tatgagtaga agatattgga
     1861 cctatagtac ttcctcctct ttcatctgac ctattaacaa attcttgata ctcaacatta
     1921 gcttctctac atatattttt ataaatagct atagaatatc catcactagt atatgcctga
     1981 ctagcattta tctttataac cggacctttt cccattattg gtttattagt tgggtcatgc
     2041 ttttctggaa tgtttggatg aatagcatga gctaaatctg atgatatcat aaaagatgaa
     2101 taaatagact caaagaatcc ctctctatcc ttacccattg atatgcaaat tctctccaat
     2161 atatttaaca acatgtttga atctgcacct tgtttagtag agctaccaac ttcttcattg
     2221 tcaaatacag ccattacatt tacaccatgt tgacctttag tattaacaag agcattcaaa
     2281 ctagcatgta ccattgcaag attatcttgt ctcccaatag atataaattc atcattagcc
     2341 cctattaaac ttcctttttc aaattcatat aaaaataaat caaaatctat aatatcgtcc
     2401 atatttacat ctaagttaga tgctatttgc tttataagaa aattatcttt ttctaatgta
     2461 tcatttaaca tcccaactaa aggtaacata tctttttgct tattatatga gtgaccatca
     2521 tttatacttc tatttaaatg tattgctaga tttggtatta tacaaattgg ctttttaaag
     2581 tctataagcc tttcatttgg ttttaatata ctatcacctt ttaaaactac tctaccagct
     2641 atcccaagag gtctatccaa ccatgtactt aatattgctc ctccatagca ttctgtattt
     2701 aacttcaaat aagatttttc agcttctatt tctgcatttg gtttaatact aaatgtagga
     2761 gaatctgaat gagagcctat tattctaaat ccatctcttt ctataccttc tgaatttact
     2821 acaaaagcta ctatagctga taaattctta gtaacataat actttccacc aactcttaga
     2881 ttccatcttt gattcaaaac taattcttca aatccatttt tatctaagat ttccttagta
     2941 ctctcgactg catgaaaaga cgacggacta tcatatatga agtctattaa gtttttagca
     3001 aagtttatac tatccattcc tcatttacct ccattataaa atatttagtg ttgtatattc
     3061 tttatttatt tttttaattc cctgttggtc aaaagctata gtaacatctg taccaattat
     3121 agaaaccact gtcccaaccc caaatttagg atgatgtacc ttagaaccaa gttttatatc
     3181 atctggatta gtttctttat tagaatcttt tattgtagca ttaactttat ctgcaacttt
     3241 agatttgctt atagtactca tatacttttt agtatattta tctaacatat tataacttgc
     3301 tttagaatag cttagttctt tttttacttt gtacaatctt tctatacatt cttctggcag
     3361 ttcttccata aatcttgaag ctattgcaac atttgtttta ccatataatg ttctcttctc
     3421 tgtcaatgac atatatagct cttctttagc tctagttata ccaacataac aaagtcttct
     3481 ctcttcttct atttgtgaat cactcataga ttttatagct cttgatatag gaaataaccc
     3541 ttcttccata cctgttaaaa acacaacagg gaactctaaa cctttggatg tatgaattgt
     3601 cataagcgaa accctatcat tgtcttcttc atcatttgat tcagatgtaa gtgctatact
     3661 tgttaggaat gtctctaagt ctttattttc ataagtatca tcattactac tttcaaactc
     3721 caaagctata gaaataaatt ctctcaaatt atcaattctg tcttgagctt cttcaccttt
     3781 gcctgttaaa tctttttcat ttttatttct tatctctaca agctcgtcca tatatcctgt
     3841 agtatccaag actttttcta taagtttgct aacaggataa acttctttta tagttctaag
     3901 agtccctata atatcaacaa atccacttat acttgccctt gccttagtag atatatctga
     3961 atttgtttct atatcaatta atactgagta tatactttcc tgttttaagt tagctctatc
     4021 ttcaattttt tctatagttc taagccctat acctcttcta ggaacattta taatcctttt
     4081 tatagatata tcatcttgtg gattttgaat aactcttaaa tacgccaaca aatcctttat
     4141 ttcttttctc tcatagaatt ttgtaccacc atatatattg tatggaattt gacttctatt
     4201 taaagcgtcc tctacaggtc ttgcctgtgc atttgctcta taaagtactg caaaatcctt
     4261 ataaggtcta ttttcttttc tagctatttg tgcaattgaa tcagcaatga aatcagcctc
     4321 ttctatttca ctaccagtta attgtatctt tataagttct ccttcttttt tttcactcca
     4381 aagctttttt ctttttcttt ctatattatt agatataact ttattagctg catctaatat
     4441 aacttgtgta gacctataat tttgttccaa ctttacaaca tgtacattgt cataatcttt
     4501 ctcaaattca agtatgtttc ttatatctgc acctctccat ccataaatac tttggtcatc
     4561 atctccaaca acacaaatat tttgatgttc tcttgctaat aattttatca attcatattg
     4621 agctttactt gtatcttggt actcatctac cattatgtat ctaaatctac ttctgtaata
     4681 tgctaacact tcatcattag ccttgaaaag ttctacagtt ttaagtatta aatcatcaaa
     4741 gtcaagtgca ctatttcttt ttaaacggtc ttgatatagc gcataaatat ctgcaatctt
     4801 aaccattcta ttatctgcca tattgatatc tttaaactgc tttggagtat aaagtttgtc
     4861 ttttgctcct gaaattgcac ttataactgc ttttggttca aacactttat cacttaaatt
     4921 taattctttt agacagtctt taaccaatgt aacttggtct gctgaatcat aaattacaaa
     4981 acttctattg taaccaatct tatttatatc ttttcttagt atacgaacac agcaagaatg
     5041 gaatgtactt atccacatat ccttagtttc aggacctata ttttgttcaa ctctctctct
     5101 catttcattt gcagctttgt ttgtaaatgt tatagctaat atatttggtg cttgtacacc
     5161 tttatcttct ataagatatg ctattcttgt tgttaatacc ttagttttac ctgaaccagc
     5221 accagccagt ataagtactg gaccttcagt cttttctact gcttctcttt gagcaggatt
     5281 taatgtatct aaattcatat ttctcctcct gttaacagcc ataaatatat tttaatctaa
     5341 aatatgaatt acttcaaatt ataattatgt attatattaa tagattattt ccaatatatt
     5401 acaattaaat aacatttttc catataattt atttcattgt ttaataaaaa aaataacctt
     5461 atgatttggg gaatcataag gcacctttaa ggggtctagg ttaagggtta ttatatataa
     5521 atctattttt ttaaaatagg tttagggctt aattatttat ttgtattaag ttctctttcc
     5581 ttccaatact tatacaaatc ttcttctttc ttaaataagg gtaaattcat atttctatgt
     5641 acttttacta accatgggga ttctaggcca gcttgtataa tattattttc atttataaac
     5701 acattttctg cttttccttc atcaatcaaa atccccttat ccaatacata tatgtaatca
     5761 catatttcat acattaaatt catatcatga ctggatatta ctatttttat attattttta
     5821 tttaatccct ttattatatc tacaatagac ctagtactta ctgggtctaa accagctgta
     5881 ggttcatcaa gtaatacaat ttcattttcc attgctataa ctgatgctat tgccactctt
     5941 tttttctgtc catagctaag aaaatgtact ggcctgtcta taaaatctat tccattaaca
     6001 gctactagag ctttgtttat tctctcttta attgtttttt catccatacc aatgtttctc
     6061 attgcaaagg ctatatcatc atatactttt gaataaaaaa tttgtttttc tgggtcttgg
     6121 aagacaatac ctacattttt tctaaggttg tataaacttc tcttatcata ttttaacttt
     6181 tcttctttaa aaagaatttc tccagatgta ggctttaata tacccattaa attcataaat
     6241 aatgttgatt ttcctgaacc atttgaacct attataccta taacatttcc ctttgaaaaa
     6301 tccatattaa tgtttaatag tgcattggta tttttttcat attgatacgt taaattattt
     6361 attttaaaca tcattatccc ctactatgtg aaatttacca tcatacaatt ttatgtctaa
     6421 tgatatacac atatcatcat accttgtcat aagtcttttg tacagcatac ttccaagaat
     6481 acctaaggat ttatatgaag tttttaaatt tatatatcca aatcttagct cttgtgactt
     6541 gtatatttct gaaaattctt ctaaaaatat gaatataaac ctatatatta acattgataa
     6601 ctcgacaaaa gtgtcaggta aatgaagttt tttaaaaaag aatattaatt gattaaaagg
     6661 tgttgtaagt atacaaaaat acacacaagt caaacaagac atcgctctaa ataacacatg
     6721 aatagacatg ttaatagacg cctttgatat tcctacatta aaactaaaaa attcaaaact
     6781 atataataaa tctgctttat taaaagatat atttacaaga gttattccta tactaagaaa
     6841 taaaaagtac attggtattc taagtaattt taaataattt tttaaatcta ttccagccac
     6901 acaaacaacc aaaatactca ttaaaatcat aattccactt aaaataaata tattttgtat
     6961 taccatagaa gcaatcagaa aaattactcc tatggctact tttttgtttg gatttacttt
     7021 actcaatcta tttgtatatg catatttatc aattataagc atgattaact tctctttcct
     7081 ttttgaagac ctaatatgta tcctattaca ccagccccta aagctgcttg tgcagagaat
     7141 aataaacttt ctatttctcc acttggtggc tcatatggac tgctaaacca tggtttgtaa
     7201 tttggattta tcttagtaat ttcactttca gcttgaccat ctgctcctcc atattcagca
     7261 cctgagttga caagtaatgg gaatataata agagctacta ctaataaaag taataatatg
     7321 ttagtttttg tttttgtttt agcactcatt agctttaact cctttatcat tattttctag
     7381 tatgcttttt tctccgtttt ctgtaattaa attatataca attacagtaa gtataccttc
     7441 tgctattgct attggtattt gagtcataaa gaaaattcct aaaaatttaa ttgctgaagc
     7501 cattacccca cctgatgggt ctgggaaagc cagtgctaat tgtattgaag ttattgtata
     7561 tgtggctaag tctcctattg ctgctgcaag aaatactgga atagcattat ttccatcttt
     7621 tttctttaaa gccttaaata ttaggaatga tacgattggc cctattatcg ccattgaaaa
     7681 agcattagct cctaaagtag ttattcctcc atgtgctaat aacaatgctt ggaatattaa
     7741 aactatagtt ccaagaacaa acattacgct tggtccaaac attattgctc caagacctac
     7801 accagtagga tgtgaacaac ttcctgttac tgaaggtatt tttagtgctg ataatacaaa
     7861 cacaaatgct ccacataaag ctaataatac tttcttttta gggtcttgct ttactatttt
     7921 acctattgat tttaatccga ataagaaaaa tggtataaat ataactcccc atgctataga
     7981 ccattttact ggtaagtaac cttccattat atgcattgca tttgatgtaa ctggagttaa
     8041 aatgattaac ataagtgcag ctataacccc taatttaata ttctgtttca tcttagtatc
     8101 cctctctccc tttctaataa ttaaaacata ataatacatg tctgatttat ttaataaaat
     8161 aaaaaagcct aactcgatgg ttaggcttct aattttccac aatctaaata caaacacata
     8221 aaacaaacat atctgtataa ttattataag aaactttctc catccccgaa gaaattactt
     8281 taatatttta ggcaggtctc ctgacttagc ttcatcctac tccttttcct tcccatgaga
     8341 tataaaaaat atcatcatag tggctaacaa aagtttcgtc tgcatcacag tagcgggggc
     8401 tgtaaaggaa tttaaccttt ttccctatta atcttgctta aagaacctat aatattatat
     8461 atttaatttt taaattattc ttaaatcatt ttctatataa atcattgtat accttttctg
     8521 tgaaaatttc tacatttttt ataaatttta ttcattttta tatttttatg tatataaatt
     8581 cttttttgta agtttattaa tttctaatta tcttctttaa atacattgaa actacatcag
     8641 aaaatctagt tatatctctt gaatatataa aatcatttcc atatattaat ctttcagctt
     8701 caagttctga ttcttttcct gtaaatactc ctaatactaa tatattttgc tttctagctt
     8761 ttctcacttc attggctgta tctctcaaac caactatacc cctataagcc ttttctcctc
     8821 ttaatgttct ctcactactt tttcctatct ttacatcatt tggtcttcca tcgctgagaa
     8881 ctatcaggat tttattttct tcatctctat ttattagacc actacatata gattttattg
     8941 ccaaaccatc tctattatta ccagcacaaa aatattcaaa tatattttca ctatttttta
     9001 tactatcatc ataatcttta aatcttttta atattgtata gtctaaaaaa ctagaaaatc
     9061 ccatcactct atttggtatg ccaacttctg ttagagcatt tgcaattatg taagcttgaa
     9121 ttgccacatt tccctgattt ctactttgag agccacttgc atccaataat atatcaataa
     9181 cgtatttccc tttttcattt tctatatctt tataaaatat tttattatta ttacttctac
     9241 caactctcca agctctattg gctaatatag taccacaatc agaatatact ctacttttag
     9301 cattttcttc tattaaaatt cttgacagac tttcttttaa tctctttatt tgctgtctat
     9361 acattctagc atgttctcta aattttccta aattattttc tttttgtctt gttacatatt
     9421 ttagttgaaa tacattatta cactcacttc taagaactcc atctgtaaag tgtattctac
     9481 aaccttcatg aacatttcta caattttgtg tctcaatttt tttaatttca cctttactca
     9541 aataagtttt tccataataa tgttctattt ttgaatatat cttatttgca gtgtcttcat
     9601 caacatatat aactcggtct gctgaattgc tacatttaga atcatcaaaa tcccctacac
     9661 cttctacaag catggatgaa gatattgaat ctatatcact ttcaataata ttttcttctt
     9721 catcactata aagttcttca tacatgaaat cagcaaatgt atcaaaattt acttctacac
     9781 tttctacatt attttcatca ataagactat tttcttctcc atttaatatg tattcaaaat
     9841 aagttaaata tataacatct aatgctttta aaatctccat aatatcttga ttggtatcta
     9901 tatttcttat atcattaact attcttctag tcatactatc taatgctggg tgcttgtcca
     9961 tactataaag tacaaatgtg tatttaattt tatccataat attatcattg tgtattttgg
    10021 taaaacgtga aacaatatct ttataagcat tccttcttat gtcttctatc cctggcctgt
    10081 ctttaatgac cttttcttca actattgtat ttaatataac ttgaattaga cctagtataa
    10141 catctttgtt gtatcctttt cttattctat ttgtaagata ttttttaact atatcccaat
    10201 ctacatactt tcttctagca cctgcaatta ttgcgaaata tagacctatt tcttttgaag
    10261 agtaatcttt ttctgatata tctatacttt cattgtaatc accagctatt gtccatgata
    10321 ggttattagc tctattttca aattcataat catcatatat ccatttcata atctcaccta
    10381 tattaaatat gacttattgg aaacacatct atactctccc atttattagg tattctagtt
    10441 ttaattacat ctccaaccat ttctttctca tatacatcaa aagtctttcc tgtaagtccc
    10501 atattaattg caagagttgg ttttaatcct cttcttatag tcttaagaga tgccataaga
    10561 ccacgtaaat caatagcttt actagatatc tctccatttt gagacttcaa ttgtaaatct
    10621 aaaaatattc ctgcaaagtg tattaatttt tcttcatcag catctgaaaa ttcatttttt
    10681 aaaatcatca ttaacttatc ttcttctata ggaggaatat ctattaccat aaatctagat
    10741 accagtgctt cattaagttc ttttgttcct gcatattcat aattcatagt cccaataaat
    10801 cttgttgctg gatgtagatt gactctctca tacccaggta catcaattat tcttctatag
    10861 tctaaagctg aatgtaccac aactatagca tcattttttg ccatattaat ttcatcaaac
    10921 accccaaaac ctccattaat agcacactca taaactgagc ctcttctaag tttaacttca
    10981 ttgtctataa atgtatctgt acctataagt gttgaactgt ctgtatttat atgaaatgat
    11041 gtattccact gaggtctatt aaatatttcc cctagattat cagcaagtaa gttttttcct
    11101 gttgcctttg gtcccgataa taatatattt tcttcttcta aaatagctgc tatacacata
    11161 gacaagatat ctttaccata aaaataagtt ttagactttg ttactctgta ttctaaatct
    11221 ttttctaaac cataataatt tctaaaatac agtacatctt caataagttt ttcatctacc
    11281 ccttgcaccc ttagattatc tattatatcc atatttttac cccttttata taaatagtat
    11341 ttgtttcatt tcttatcacc attttagcat aaaaggatat aagctaaaat tgatttttta
    11401 atctccccat tttaagtaaa accatgcacc attttatcac tacaaaatta agagtactac
    11461 ttactgatat ttcttgattt tgttcgtcta tacgtatata atatatgctg ataaattatg
    11521 aggaaaggat gattgaaata tggatgccca gaagtagcaa aaaaatgggg tatttctgaa
    11581 agaagagtac aaaaactatg tgaagaaagt cgcataccag gagtttcaaa actcgggtat
    11641 atgtggctga taccaaagaa cgcagaaaag cctgttgatg gtagaacaaa aagaagaaag
    11701 gaagtacaat atgaatagcg ttttgattat agatgatgac aaagaacttt gtgctcttat
    11761 gaaaaaatgt gttgagcaag aaaatttatc tgctattact gcacattgtg gtgcagaagg
    11821 tctacatttg gtcgataaaa atagacacac actctctctt gtaattttgg acataatgat
    11881 gccagatata gacggttttc aagttctgaa aaaaattaga gaaacaagta atattccagt
    11941 aattatgctt actgctaaaa atgacgagga tgataaagtt tccggactac gtttaggtgc
    12001 tgacgattac ctaacaaaac cgttcagcat aaacgaactt atggctcgtg tcaattctct
    12061 tattcgtcgg tatactatgt taaaccctgc ttttataaca gacacagatt gcataataat
    12121 aaatggcatg attattgaca agacaaaccg aattgtttct gtcaataata ttcctgttca
    12181 acttacaagc aaagaatttg atttacttgc ttttctggct tctaataagg gacgggtgtt
    12241 taccaaaaaa cagatttata cacaagtatg ggaagaagaa tatgcttttg acgatagtaa
    12301 catcatgtct ttcatcagta aattgagaaa aaagattgaa ccagacccca accatccatt
    12361 ttatattttg acagttcgtg gtgtcggtta tcgttttaat aaggaggctt aacatgaata
    12421 caagtgtcta tctgcttttg atattggttc ttagcctgat tattatagcg taccttgttt
    12481 ctgtcctgct acgtgtccga atacaactta cacttataaa agatgcttta gaggatataa
    12541 aaaatggaaa ccttaatcga cgtattttaa cagatgaaaa cgacattaca aaacaaattt
    12601 gctatgatat taacgaaata gcaatcaata gccaatttca acttattcag caaaaacaat
    12661 cagaacaagc ctacaaacag ctaatgacaa gtctctccca tgatgtaaaa actccacttg
    12721 cttctttggt cggttatttg gaagcagtag aaagtaaaat tgttgtagga gaggaaaaag
    12781 acgagtatat ccatgttgcg tctaataagg ctcattactt aaagaatttt gtagaaaatc
    12841 tatttgaatg ggtaaaattg gattctaagg aacaagtttt tcattttgat atttttgact
    12901 tgaatgaatt atcccgtaat attatatctg attggatacc tgttttagaa agcagtcatt
    12961 ttgaatatga atttgatata ccagaaatag agtacttctt acgtatagac gcaaatgcat
    13021 atacccgtat cattaataac ctattacaaa atattattac ccatagtagt ggcaacaaaa
    13081 tgacattgag aatttttgaa aacaaagagc aagctcaaat tataataaca gataatggga
    13141 aaggtatatc ttcagacaat ctcccacata tttttgaaag gttgtaccaa tgcgaccatt
    13201 cacgagcttc aaagggaaat ggtttaggct tggcgattgc aaaggaactt attaacgctc
    13261 ataagggaaa tattaccgcc aacagcactc ccggtatggg aacagaattt acaatattgc
    13321 ttcctaaagc cctgtaaaag tacgaggctt tcttactcta aaacaagata aaagcaagat
    13381 tatggcaagg ttttggcaag gttcatgttt tataattcta attgtagttg attttgacgt
    13441 tgtatcaact atcttttgta gaaacaaatt tattaaaagt gaggaaatta tatatgaatg
    13501 attttgtgat tgaaacaaag caacttacaa aaatttatgg gaatcaaaca gtagttgaca
    13561 aagtcaatct ccatgcaaaa aaaggccgaa tatatggtct gttaggacgc aacggagctg
    13621 ggaaaaccac aattatgaaa atgattttag gattgacttc aatcacgtat ggcgaggtag
    13681 atgtatttgg agagaatatt aagggacgtg aaaaacaagt atatccccgt atcggtgcaa
    13741 ttatcgaaac gccaggattt tatcctaatc tgacaggaac ggagaacttg gagatttttg
    13801 caaaactgcg tggtacagcc gcccctaatt ctgtaaaaag agcattggaa attgtaggct
    13861 taccttataa aaataaaaag ctgtttagtg aatattcact tggcatgaaa cagcgacttg
    13921 gtattgccaa cgctatcctg catgacccag aactgttagt tttagatgag ccaaccaatg
    13981 gtcttgaccc tattggtatt gcggagatga gaaagtttat tagggacttg agcgttgagc
    14041 atggaaagac aattcttatt tccagtcata ttctttcaga aatctcattg ttggctgatg
    14101 atattggaat tatcgaccac ggtgttcttt tggaagaaag tagtatgagt gaactagaaa
    14161 agaaaaacag taaatatatc ctgctacaag tttcagatat tccgaagaca tcacttattt
    14221 tagagcgtca atttaacacg aaggattatt ccgttcaaga tgactacacg ttacaccttt
    14281 ataacactaa tctggatatg ggtgaaatca ataaagcact tgtaatgcag aatgtaactg
    14341 ttattagttc tcaactttgt aatgatactt tagaggacta ctttaaaaaa atcacaggag
    14401 gagagggcat tgcttaaact tattcaaaca gaatttctta aactgcgtcg taggaaactt
    14461 atttggctga tgttgttgtc tgctctcatt atgccgtttt tcgcactact atacttcaat
    14521 tattttggta aaacaggtgt tgagccaata aagttttata aatggtctgc atttggttat
    14581 acactttgga ttattttgcc tgttgttttg ggtatatttt gcaccatgct tatacacgaa
    14641 gaaaaccagt atgatatgct caaacagctt tggattgtac ctattagtaa aatgggatac
    14701 ttttttagta aatttttcgt tgttttgatt tactctattt gttttatgct gattacagcc
    14761 gttgcttctg tgttatttag tgtactttct ggatatgtcg tatttagctg ggcaagcatt
    14821 ttatatttgt tgaaaaaatg tttagaaatt ggtgtaatca ctgcttttgt aatgttgccg
    14881 attttggcaa tcgctacatc tcaaaaagga tacattctcc caatttgtat cacattggta
    14941 tatgcctttt ccggttttgt tataatgaca ataaatatgt atttacaccc attatccagt
    15001 atgggagtta ttatcatgag aaataaagac attccaggtc ttatattttc acaggaaatt
    15061 aatgtcccat tagcatttct ttgtatttgt atttggagta ttgtatcagt attgtttgca
    15121 aacactgcac taggaagaag aaagtgaggt gtagataatg tggaaattac tttgggctga
    15181 atgtcaaaaa attcgtcgtt caaaaattgt ttggattaca gtttttgcat ccattatgat
    15241 agcagtcata gtttttgcag gagggcagga tgtatataat gacccagatt tacattatgg
    15301 cttaaagagc gtttatgacg gttcacggta tatagacaat gcaggttggt atatggacgc
    15361 agtacaacct tggtcaactt tttttgttct tcctgctgtc attgccctat taggaagcta
    15421 tattatttgt cgtgaagaag aggaggacac tattaaatcc ctgcgtctta ttcccataaa
    15481 tgaaacaaaa ttaaccatag caaaaatgat tattactttt atattcagta tacttcttta
    15541 tttgttactc tttactataa catttttgac tgaatccgtt ttacattttt ccgatttatc
    15601 gacaaaactg gttttaagtt gcatgaaaga gtattttctt gatggcatag gtgtattctt
    15661 tgcaatttct cccattattg ccttggtatc gcgcatgaaa aaaggatatt ggcttgcatt
    15721 agtattcaca gaagtttatt ctattgctgg actatttgcg ggtatgtcaa atgtcttaca
    15781 aacctttttt cctattgcgg caatatttaa tatttctgga tactatataa ctactggaga
    15841 aaaaattatg gggagtatca taagtttatt actttgtgcc tgcctttctg catttatact
    15901 taaagggcta aaacacaatg aaaaaaattg aaataggtat atcaaaatga aaaagataaa
    15961 aaaactgatt attctaattg tattcttgtt tctttttggc atgttacact gttatactga
    16021 atggaaattc aacaataacc cacctaaagg ggtaatagaa agaataaatg aatggactga
    16081 taagattgga ctggattagt aacttatgtt aattatagtg aatgtaatat tagcaagtta
    16141 ttgtttcgta ttttaatact ataactttaa atatttggtt ttaatcttcc aaacgaatat
    16201 aaaaaaactg tcacagaggc aacatatttt tgaatattta taaagtggta gttttgattt
    16261 tcaaagctgc cgccttttct tctatataag caaacttatc ctcaacaaag tgtaaaacat
    16321 ggtacatttt aagaaaaaag taccacagac acaagcaata aaagaaacgc cgagaaccta
    16381 caaaaacgta gtgcttatgc agatttacga cgccttctat tttctcaacc gtcttaatga
    16441 ccttgtacaa aagaaatttt tctaatattt tttataatct tatcttgtga tgttttttat
    16501 gtataaatga cttaataaca aatccaataa aaattatgga tattggtata attagaacga
    16561 ggaatttata atcattataa tgtgacatta ttcttaccca actctcagaa aaaatatatc
    16621 ctatactgat taatgaagta ttccatatgg ttattcccac gatagatgat attacaaaag
    16681 ttataatatt ttgacctgct atacctgcta taaaagatat gtatgttcta cataaaggga
    16741 ttaatctaca tacacaaaca gaaatagaag agtatttgtt aaaataatct tgagatgcaa
    16801 atattccttt ctttgccttt ggacatatct ctattatttt atttatgata gatttacctc
    16861 cccaatttcc cactgtataa caaaatatcg aaccaagtat acctgcaatt atacttaaaa
    16921 ttaaaattgt cagaaatcca aaatgatttc ttgcagcaat tgctccacat aaaggtaaaa
    16981 caacttcgct aggtaatgga aaacaagcat actcaatcat aattaataca aatacagaga
    17041 ttaatccata ttgtaaaata aaactattga ttacagacac ttacttatct atctcctttc
    17101 ttatttaaag gttttaaggg tacatgttct aaggattctc accctatatt taaagaatca
    17161 atctctttaa actattatat gtatttacac aaagcattta taacatactt taaaaatagt
    17221 accttaaaat aagaaactta ataaataaaa aaagattacg tggctacgtc ctactctccc
    17281 aggcag
//
LOCUS       DAMJYR010000332        16275 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.guided.086, whole genome shotgun sequence.
ACCESSION   DAMJYR010000332 DAMJYR010000000
VERSION     DAMJYR010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 16275)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16275)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16275
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.guided.086"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            246..863
                     /locus_tag="PC471_003972"
     CDS             246..863
                     /locus_tag="PC471_003972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HDF2790996.1"
                     /translation="MCKKTYLLVVSMLIAGLLTACSGSPTKDSGQAQDNTKKEANASN
                     NALKDEKNNENLMEQDFKVPYTDAINIFKDKYKDADIVDLSLERDLNKFVYTVEGVDD
                     NNEYKMKIDANTKDVLEDKTEKLDSEDLNGVARKEKLDLNDIMTPQQAMEIALKEQNG
                     IVKEWSLDKDLDVTFYKIRIDKDKNEYDIKVDSKKGTVLKVEKED"
     gene            971..3502
                     /locus_tag="PC471_003973"
     CDS             971..3502
                     /locus_tag="PC471_003973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HDF2790997.1"
                     /translation="MSLKSLKIKENLYWVGSLDPDLRVFDIIMYTPYGTTYNSYVLKG
                     TEKTVLFETVKDKHFDNYIERLNDLNIDFEKIDYIVVSHTEPDHAGSVEKLLDLAKNA
                     KVVASETAIKYLKEIVNKDFEYVAVTDGDTLSIGDKTLEFFSVPMLHWPDTIYTYIKE
                     DKTLVTCDSFGSHYSNDKIVNTLDENEEKDYLDALRYYYDCIMGPFKPSMVTAIEKIK
                     DLDIDTVCPGHGPVLTENPRKIIDLYYNWSVNEQIKLEKEVTICYVSAHGYTKIMAEA
                     IKAYIEKNSNYKVNLFDVIEHKQEDILAKIAVSQGVLFGTPTILGDALKPIWDILISL
                     NPVLHGGKVASVFGSYGWSGEGIENAMERISQLRMTAVKPFAVNFKPSNEEIDKLYSY
                     TGKFLDKLNSTFGSKKKTKKFKCVICNEVFEGDSAPSVCPVCGAKEDQFIEVEEDEVT
                     FRKDTDEYFVIVGNGAAGFYAADAIRKRNKTCKITMISNEDELTYYRPALSDGINEEL
                     GSDFYMEDKDWYDKNNIVVILGTNVDKLDEVNKTIIVNDGAIKFDKLVIATGSRNFIP
                     PIKGHDLENVFTLRNIKDLYSVKEALEKSKKVVVIGGGLLGLEAAWEFRLKGLEVVVV
                     EAMDSILSKQLDKEGSKILEQCVRDTGIDVRLGVAVDGIEGDGKAQKVVFKDGDSVDC
                     DMVVFSIGVRANTQMVQDTSVKIDRGIVVDKTLQTNVKDIYACGDVAQVGNISLAIWP
                     SSVEMGKIAGANASGDNLTFESEVYPVSLDAMNVKVFSIGNIQNFDKEISSKDEGQRI
                     YKKLFMKDGSLVGAILINDLSCTVKLIRLISEKGDFEDIMKADIL"
     gene            3936..4637
                     /locus_tag="PC471_003974"
     CDS             3936..4637
                     /locus_tag="PC471_003974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021418928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanG-Cd-type vancomycin resistance DNA-binding
                     response regulator VanR"
                     /protein_id="HDF2790998.1"
                     /translation="MNENILIVDDEKEIADLIEVYLKNEGFTVYKFYTGKEALECIES
                     KNLDMAILDIMLPDIDGFHICQKIREKYYYPIIMLTAKIEDIDKIMGLTIGADDYITK
                     PFNPLEVVARVKTQLRRYMRYNNSYEQQSIIVNEYDIKGLIINKETHKCSLFGKEVAL
                     TPIEFSILWYLCEHQGKVVPSEELFEAVWGEKYLDNNNTVMAHIGRLREKIHEPSKNP
                     KFIKTVWGVGYTIEK"
     gene            4687..5769
                     /locus_tag="PC471_003975"
     CDS             4687..5769
                     /locus_tag="PC471_003975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF2790999.1"
                     /translation="MRTALMAFIAIIFIWILYSFILYGKFSIWIVSFFENAFRMEYKA
                     ALNLYQQIFRNSMELFMILAMVIVFFIIFRVYLNWFTKYFSEINQGIDSLIKEDVGEV
                     ALSPELLAIEKKINSIKHILEQRKFETQMAEQRKNELIVYLAHDLKTPLTSVIGYLTL
                     LRDESQISEELRKKYLSISLDKAERLEDLINEFFEITRFNLSNISLEYSTVSLTRMLE
                     QLTYEFKPTLMSKNLKYTLNVTPNMMIRCDVNKMQRVFDNLLRNAVYYSFEDTTIEIT
                     AVQEDDYIKLKFINHSNTIPEEKLERIFEQFYRLDTSRNSNSGRAGLGLAIAKEIVEL
                     HNGTITAHSENNIIIFKVIIPIVGNL"
     gene            6030..7130
                     /gene="vanG"
                     /locus_tag="PC471_003976"
     CDS             6030..7130
                     /gene="vanG"
                     /locus_tag="PC471_003976"
                     /EC_number="6.3.2.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021418924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-serine ligase VanG"
                     /protein_id="HDF2791000.1"
                     /translation="MNNKKIAVIFGGNSTEYDVSLQSAFAVLENLNTEKYDIIPIGIT
                     REGNWYHYIGDYNNIQNNTWFERDTDIIPVIVSQNRVKNGIIEFLIPDAVSQNHIPNS
                     AIESCENDIKYTKLDFVFPVLHGKNGEDGTVQGLFELAGIQVIGCGTLSSALCMDKDK
                     SHKLVHSEGIKVPKSILLKYSDKFNVAELSKDLTYPLFIKPVRAGSSFGISKIYKKEE
                     LQVAVDLAFEHDNEVIIEENIDGFEVGCAVLGNDDLLVGRVDEIELSKGFFDYTEKYT
                     LKSSKIHMPARIDLDCEKRIQETAKIIYRTLGCSGFARVDMFLTPSGEIIFNEVNTIP
                     GFTSHSRYPNMMKGIGLSFEEILDKIIEVYIK"
     gene            7127..7933
                     /locus_tag="PC471_003977"
     CDS             7127..7933
                     /locus_tag="PC471_003977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HDF2791001.1"
                     /translation="MMNNILLDKNKEDKMTNTILLTKDKVYDGNLILVNAFLPVKTSE
                     DIDLIPVDTRFPSILMKREATNILQNILKSICGINEIVPVSGYRTAEEQQDIYSSSLR
                     DNGKDFTKKFVALPNHSEHQTGLAIDLGLKKEVIDFICPEFPYNGICNEFRKAALHYG
                     FIERYQCKKEEITGISQEPWHFRYVGYPHSEIINKMNLALEEYIEYIKRFSYTNCLKI
                     EDKEKIIEIFYVPIHTKEEVRISIPEQCVYQISGNNKDGFIVTFWRNRKD"
     gene            7951..10089
                     /gene="vanT"
                     /locus_tag="PC471_003978"
     CDS             7951..10089
                     /gene="vanT"
                     /locus_tag="PC471_003978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021418922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine racemase VanT catalytic subunit"
                     /protein_id="HDF2791002.1"
                     /translation="MIRNKSYAGLDYFRIIAALLIIAIHTSPLLSYSKMADFVLTSII
                     ARIAVPFFFMLSGFFLISTYNYNCNKLKSFLKNVLFIYGIAIVIYIPLNIYTGYFSMN
                     NLVPNIIKDIVFDGTLYHLWYLPASIIGASIAYFLVKRLGLKYSLVITIVIYVIGMFG
                     DSYYGFLEKIPLLKNLYGYIFEVSDYTRNGIFFAPVFFILGGIISNKSNYMSTKNSLI
                     GFLISFLLMLCEGMFLHKLGVQRHDSMYIMLLPCMYFLFTALTFLKGSRARLLRTSSL
                     IIYIIHPMMIVVIRMFSKIFGIQTVLVDNSLIHYIVVSIASFIASIIIILILKPLKKD
                     LKGEHKVNTNRAWIEIDLNNLEHNVKMIQKAMPEDCELMAVVKANAYGHSAFEVATCA
                     NQIGVKAFAVATIDEGIDLRRYGILGEILILGYTNPIRAKELHKYNLTQSVIDLNHAI
                     SLNKQKYNIKSHIKIDTGMHRLGFDVNDVRSILKAFKLKYLDICGIFTHLCVSDSLKD
                     EDVDFTNKQIECFYKLIDFLLKKGIKIPKVHIQSSYGFFNYPNLKCNYVRIGIALYGI
                     LSSPNDTTKLQLDLRPVLSLKSQVILIRKIQKDDSFGYGRVFIASRDSVIAILSIGYA
                     DGIPRNLSCGKSYVIINGCRAAIVGRICMDQLAVDITDIPNVEVGNTAIIIGRDNLSE
                     LSASEVANNSCSISNELLSRVGRRLNVIKK"
     gene            10403..11023
                     /locus_tag="PC471_003979"
     CDS             10403..11023
                     /locus_tag="PC471_003979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HDF2791003.1"
                     /translation="MQYIQELLDLTQNYWVLATIIGLLSAFIESFIPALPLVAIVTAN
                     AAIQGLFIGCLLSWIGSGLGTTSLFLLISRFNDSKLFNKLRNSKTEKAISWMDKQGFK
                     LLFIAYACPFMPGCLVTIASAFGKKDLKDFVPAMLAGKFVMFIVISYVASDIEGFITS
                     PLKIASFILLVFLSWKIGNRVNKNLENHNYDFHHKKHHNDKDDKMI"
     gene            complement(11189..11725)
                     /locus_tag="PC471_003980"
     CDS             complement(11189..11725)
                     /locus_tag="PC471_003980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HDF2791004.1"
                     /translation="MIKETHERGGYIFALLILPFINNLYLVKYDIAYKIVLIIIYIYF
                     AYLGSLFPDIDMRGSYISKKFMLIYKLFGSRFRHRGFTHSLIALLLIYSFFKSLTIFT
                     NNNIVFSCLSSGFIIGYLSHLCLDLITKEGIELFYPITINISLLPIKTNSKTEKFISK
                     LLNFIVIFLIGYQFYILL"
     gene            complement(11838..12512)
                     /locus_tag="PC471_003981"
     CDS             complement(11838..12512)
                     /locus_tag="PC471_003981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000094049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HDF2791005.1"
                     /translation="MSLFIISQCITSFAFTPENNKFKVKPLPYAYDALEPYIDKETMK
                     LHHDKHYQAYVDKLNAALEKYPELYNYSLCELLQNLDSLPKDIATTVRNNAGGAYNHK
                     FFFDIMTPEKTIPSESLKEAIDRDFGSFEKFKQEFQKSALDVFGSGWAWLVATKDGKL
                     SIMTTPNQDSPVSKNLTPIIGLDVWEHAYYLKYQNRRNEYIDNWFNVVNWNGALENYK
                     NLKSQD"
     gene            12715..14589
                     /gene="asnB"
                     /locus_tag="PC471_003982"
     CDS             12715..14589
                     /gene="asnB"
                     /locus_tag="PC471_003982"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HDF2791006.1"
                     /translation="MDGNISIYYKGDISKKEDIKFKIKESVNNTRLSSRIEYKDEEKT
                     FDFERFNVIDAIYGNKPFEKDGKVIVFNGNIYNYHEIKEELIKKGHNFTTNDDIEVLL
                     ASYIEFGKNCVHKIKGMFNFIIYDRENESIFGARDLFGIKPLYYINKENAIIFSSEYK
                     FLLEYMKNLNINERSLQSYFSFQYVLPEDTMIQGIRLIPAGHYFRVENGILSLKRYNK
                     LEFRSSTKFFYTKNHLGNRDVNEDDIRNIVVDSIVTHMEEEKEIGTFLSGGIDSSIIT
                     TVASQINPNIKSFSAGFSVKGYSELEVAKKTADKLGIENIQINITQDEYIKSLPNVIY
                     SLDDPIADPSEVGLYFLIKEAGKHVKVALSGEGADELFGGYNIYKEYSTMKSVVNSPT
                     YIKSILGRVSELMPNIKGRNYLYRATTPLEKRYIGNAKVFENSEVKRFFFKYKEKNIY
                     EYLLSNLYRDAQKNNYDYISKMQHIDVNTWLQGDILQKISKLSTAEQVELRVPFLYKD
                     VFDVAKNLRMEQKINKNNTKVLLREAFREIVPEHVVQRKKLGFPTPIRVWLKDSLGDI
                     VKETIYNSNVDEFIDKKYAIKLLDTHLKGHRDNSRKIWTIFTFCLWHQLFIEHKNVEY
                     "
     gene            complement(14681..16117)
                     /locus_tag="PC471_003983"
     CDS             complement(14681..16117)
                     /locus_tag="PC471_003983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HDF2791007.1"
                     /translation="MICTIKPLKISIKKSTEPLIEFISDTTYKNKSSSYIKEVYFTRY
                     IGVDDSIDKYIEKIKNIDTYFFNNPTIPYIRLNNLKINFDKEQTDKMLKIFNIYNSSK
                     FNSWDLYNIDFPCKIENDTLNWTKKIAFKNTLDLFSTSMPNCTSSIIKNFAVKLLCWI
                     DYFLPKLFYNKVKTTISPKIVYFGNIKRQELFFLYFLSQLGCDILYINPNKDILDLYP
                     ECSKFSTLFELSRKTPNILEIPFERHITNINYSENTNKTSPTTNKPNSKNTLNNTNLT
                     SKKIVQDEEVELSYEDLAKLSASVVMIVVCNNENKPFKSGSGVVINNEGYILTNLHVV
                     NDGYSFLVRFENDDKVYTSYQIIKYHSDYDLAVIKVDRKCKPIPVKVSKKPVRGQKIV
                     AIGSPLGLFNTVSDGIISAFRDFETVQMIQFTAPISSGSSGGALLDMFGNLLGLISAG
                     YDDGQNLNLAVESSLVKIFANNFIEIVN"
BASE COUNT         6209 a         1774 c         2723 g         5569 t
ORIGIN      
        1 caaaatgaac tttttagttc atgaggcact ctttttgtac gaaataaaat atatttttat
       61 tatttcaaca atgaaaaaga ggcttgtttc cattttgaga cagtataatt tttatgtaac
      121 tttaatttta gtaaatataa agttttaaaa ttgataaaat aggaggaatt tttaatataa
      181 aagggtaaat aaagggttta gagggcataa tatagaataa attaatatat ttaaggaggg
      241 tttttatgtg taaaaaaacg tatttattag tggtaagtat gcttatagcg ggtttattaa
      301 ctgcatgtag tgggagtcca actaaggatt caggacaagc acaagataat acaaaaaagg
      361 aggcaaatgc atcaaataac gctttaaaag atgaaaaaaa taatgaaaat ttaatggaac
      421 aagattttaa agtgccatac acagatgcaa taaacatatt taaagataaa tataaggatg
      481 ctgatatagt agatttaagt cttgagagag atttaaataa atttgtttat actgtggaag
      541 gtgtagatga taataatgaa tataagatga aaattgatgc taatacaaaa gatgtattag
      601 aagataaaac agaaaagttg gattcagaag atttaaatgg agttgctaga aaagaaaaat
      661 tagatttaaa tgacataatg acacctcaac aagctatgga aattgcatta aaagaacaaa
      721 atggaatagt taaagaatgg agtttagaca aagatttaga tgttactttt tataaaataa
      781 gaatagataa agataaaaat gaatatgaca taaaagtaga ttctaaaaag ggaactgtat
      841 tgaaggtaga aaaagaagat tagaagattt aaactatctt taaaataatt tcttttttga
      901 ataaattttg tttaaaatat aacaagcagg gtataaagag actaaataac tattttagga
      961 ggagtacggt atgtcattga aatcactaaa aataaaagaa aatttatatt gggtaggttc
     1021 gcttgaccct gatttaagag tatttgacat aattatgtat acaccttatg gaactactta
     1081 taattcatat gtattaaaag gaacagaaaa aacagtttta tttgagacag ttaaagataa
     1141 gcattttgac aattatattg aaagattaaa tgatttaaat atagattttg aaaaaataga
     1201 ctatattgtt gtaagtcata ctgaaccaga ccatgcagga agcgttgaaa agcttttgga
     1261 tttagctaaa aatgcaaaag tagtagcttc agaaacagca attaaatatc ttaaggaaat
     1321 cgtaaacaaa gactttgaat atgttgcagt tacagatgga gatacattat caattgggga
     1381 caaaacttta gaatttttct ctgtacctat gcttcattgg ccagatacca tatacacata
     1441 tataaaagag gataaaactc ttgtaacatg tgattcattt ggtagtcact atagtaatga
     1501 taagatagta aatacacttg atgaaaatga agaaaaggat tacttggatg ctttaagata
     1561 ctattatgat tgtataatgg gtccatttaa accatctatg gtaactgcta tagaaaagat
     1621 aaaagattta gatatagata ctgtatgtcc aggtcatggt ccagtattaa ctgaaaatcc
     1681 tagaaaaatt atagaccttt attacaactg gagtgtaaat gaacaaataa agttagaaaa
     1741 agaagttaca atttgttatg tttctgcaca tggatataca aagattatgg cagaagctat
     1801 aaaagcatat attgaaaaaa atagcaacta taaagtaaat ttatttgatg taatagagca
     1861 taagcaagaa gatattttgg caaaaatagc agtatcacaa ggtgtattat ttggaacacc
     1921 aacaatatta ggagatgcgt taaagcctat atgggatata ttaatatcgt taaacccagt
     1981 tcttcatggt ggaaaagttg cttcagtatt tggttcatat ggatggagtg gagaaggtat
     2041 agaaaatgct atggagagaa taagtcagct tagaatgaca gcagtaaaac cttttgctgt
     2101 taactttaaa ccatcaaatg aagagattga taaattatat tcttatacag gtaaattcct
     2161 agataagtta aattctactt ttggaagtaa aaagaaaact aagaaattta aatgtgtaat
     2221 ttgtaatgaa gtttttgaag gtgatagtgc tcctagtgta tgccctgtat gtggagctaa
     2281 ggaagaccaa tttatagaag ttgaagaaga tgaagttact tttagaaaag atactgatga
     2341 atatttcgta atagtaggaa atggagcagc tggtttttat gcagcagatg ctataagaaa
     2401 aagaaataaa acttgtaaga taactatgat ttctaatgaa gatgagttga cttactatag
     2461 acctgcactt tctgatggta taaatgaaga acttggttca gatttctaca tggaagataa
     2521 agattggtat gataaaaata atatagtagt aatattagga acgaatgtag ataaattaga
     2581 tgaagtgaat aaaactatta tagtgaatga tggagctatt aaatttgata agcttgtaat
     2641 agctacagga agtagaaact ttataccacc aattaagggc catgatttag aaaatgtgtt
     2701 tactcttaga aatataaaag atttatatag tgtaaaagaa gcactagaaa aatctaagaa
     2761 agttgtagtg attggtggcg gtttattggg acttgaagct gcatgggaat ttagactaaa
     2821 aggattagaa gtagtagtag ttgaagccat ggatagcata ctatcaaaac aacttgataa
     2881 agaaggtagt aaaatattag aacaatgtgt aagagataca ggaatagatg taagattggg
     2941 tgtagcagtt gatggtattg aaggtgatgg aaaagctcaa aaagttgtat ttaaagatgg
     3001 agatagtgtc gactgtgata tggtagtatt ttcaattggt gtaagagcaa acactcaaat
     3061 ggttcaggat acatctgtaa aaattgatag aggtattgtt gtagataaga cattacagac
     3121 aaatgttaaa gatatatatg cttgtggaga tgttgcacaa gtgggaaata ttagtttagc
     3181 aatatggcca tcttcagtag aaatgggaaa aatagcaggg gcaaatgcaa gtggagataa
     3241 tttgacattt gaaagtgaag tatatccagt ttcattagat gcaatgaatg taaaagtatt
     3301 tagtattggt aatatacaaa attttgataa agaaatttca tcaaaagatg aaggtcaaag
     3361 aatatataaa aaattattta tgaaagatgg ttcattagtt ggagctatac ttataaatga
     3421 tttaagctgt actgttaaat tgataagatt aatatctgaa aaaggtgatt ttgaggatat
     3481 tatgaaggct gatatactat aatttaaata cattaaaata aaaaggagtt gtctcaaaat
     3541 tgattttttt ctaatcaaat tttaaattca actccttttt tgaattttta tataaaataa
     3601 catggaaatt ttagtaatgt ctgtggttac atacataggt ttaattgttt tttatatgga
     3661 ttgtctctgg tataataaat tgaattgtgt ttgactgata gatattttta ttgaaagtta
     3721 atacctgtta tgcgataata aggacgtgtt aggggagttt ttataataac aattaatctc
     3781 agtttttgtg ttccaatagt agcatcaaca cattctgatg tagtagttct tttcttataa
     3841 aaattagtta caaatatgct tgtaattatt tgatatgtaa atttaccaat attataaatg
     3901 atgacctcga taataaggag agtgaaaaat aatctatgaa cgaaaatatt ttgattgtgg
     3961 atgatgaaaa agaaatagca gatttaattg aagtatattt aaaaaatgaa ggttttacag
     4021 tatacaagtt ttatacaggt aaggaagctc tagaatgtat tgaatctaaa aatttggata
     4081 tggctatttt agatattatg cttcctgata ttgatggatt tcatatttgc cagaaaattc
     4141 gagaaaaata ctattatcct atcattatgt taacagctaa aattgaagat atagacaaga
     4201 taatgggttt aaccataggt gctgatgatt atataacaaa gccatttaac cctttggaag
     4261 ttgttgcaag agtaaagaca caattaagaa gatatatgcg atataataat tcttatgaac
     4321 aacagtcgat aattgttaat gagtatgata ttaaaggatt gattatcaat aaagaaacac
     4381 ataaatgtag tttatttggg aaagaagtag cattaacacc gattgaattt tctattcttt
     4441 ggtatttatg tgaacatcaa ggaaaagtgg ttccttctga agaattgttt gaagctgtat
     4501 ggggagaaaa atatcttgat aataataaca cagttatggc tcatataggg cgattacgtg
     4561 aaaaaattca tgaaccttct aaaaacccga aatttataaa aactgtatgg ggagtaggtt
     4621 atacaattga aaagtaaaaa tattaaagtc aatcctgact ttactcaagt gagaaataaa
     4681 atttttatga gaacagcttt aatggcattt atagccatta ttttcatatg gattttatat
     4741 tcttttatat tgtatggtaa gttttctatc tggatagtat cattctttga aaatgctttt
     4801 cgtatggaat ataaagctgc attgaattta tatcagcaga ttttccgtaa tagcatggag
     4861 ctcttcatga tactagctat ggtgattgtg ttttttataa tattccgggt ttatttaaat
     4921 tggtttacaa aatattttag tgaaataaat caaggtattg actctcttat caaagaagat
     4981 gttggagaag tggctttatc tccagagtta ttggcaatag aaaagaaaat aaactcaatt
     5041 aaacatatac ttgaacaacg taaatttgag acacaaatgg cagaacaacg taagaatgaa
     5101 cttattgttt atcttgcaca tgatttaaaa acaccactta catcagttat agggtatctg
     5161 acactacttc gagatgaaag tcagatttct gaagaactgc gaaaaaaata tttatctatt
     5221 tcgttggata aggcagagcg tcttgaagat ttaattaatg aattctttga aattacaagg
     5281 tttaatttat caaatatatc acttgaatac agtacagtaa gcttaactcg tatgcttgaa
     5341 caactaacat atgagtttaa acctactttg atgagtaaga atttaaaata tacactgaat
     5401 gttacaccaa atatgatgat aaggtgtgat gttaataaaa tgcaacgtgt atttgataac
     5461 ttattacgaa atgctgttta ttatagtttt gaagatacta ctattgaaat aacagctgta
     5521 caggaggatg attatataaa gcttaaattt ataaatcata gcaatacgat tccagaggaa
     5581 aaacttgagc gtatttttga acaattttac aggttggata catctcgtaa ttcaaacagt
     5641 ggtagagctg ggttgggact tgctatagct aaagaaatag tggagcttca taacgggaca
     5701 ataacagctc atagcgagaa caatataatt atctttaagg ttattattcc gattgtagga
     5761 aatttgtaag aatttaagta taaaaaaata ggaaattctt taggataaat aaataaagag
     5821 cactcttgtt attgatataa ttactgtatc aaaacaagag tgcttttatt ttgatacagt
     5881 aattatatca atagaaaaat gtgaaaatat attatgtgga acagatgcaa ttgtaagtga
     5941 aggttgattg gatttgaaat agaaaaagta ttttaacatg tttctgtaga ttgtataaaa
     6001 ataaataaat caacaaagga gaatcaaaga tgaataataa aaaaattgca gttatatttg
     6061 gtggaaactc aacagaatat gatgtatcat tacaatcagc ttttgcagta cttgagaatt
     6121 tgaatacgga aaaatatgat attataccaa ttggaattac aagggagggt aattggtatc
     6181 attatattgg agattataat aatatccaga ataacacttg gtttgaaagg gatactgata
     6241 taattccagt tattgtttcg cagaaccgtg tcaaaaatgg tattattgaa tttttaattc
     6301 ctgatgctgt ttctcagaat catattccaa atagtgctat tgaatcatgt gaaaatgata
     6361 ttaaatacac taaacttgat tttgtgtttc ctgtactgca tggaaaaaat ggagaagatg
     6421 gaacagtgca ggggttattt gaattggcag gtatacaggt tataggatgt ggtactcttt
     6481 cttcagcatt atgtatggac aaagacaaat cacataaact tgttcattca gaaggtatta
     6541 aagtaccaaa atcaatatta ctaaaatact cagataaatt taatgtggct gaactgtcaa
     6601 aagacttaac ttatccatta tttataaaac ctgtgcgtgc aggttcatca tttggtatta
     6661 gtaaaattta taaaaaagaa gagttacaag tagcagtaga cttggctttt gaacatgata
     6721 atgaagtaat aattgaggaa aatattgatg gttttgaggt agggtgtgca gttttaggaa
     6781 acgatgactt acttgttgga agagtagatg aaatagaatt gtcaaaagga ttttttgatt
     6841 atacagaaaa atatacattg aaaagttcaa aaattcatat gccagctaga attgatttgg
     6901 attgtgagaa aagaatacaa gagacagcaa aaattattta tagaactctt ggatgttctg
     6961 gatttgctag agtagatatg tttttaactc catctggtga aattattttt aatgaagtca
     7021 atacaattcc tggctttact tctcatagtc gttatccaaa tatgatgaaa ggtattggat
     7081 tgtcttttga agaaatattg gacaagataa ttgaggtgta cataaaatga tgaataatat
     7141 tttattagat aaaaataaag aagataaaat gacaaatact atcttattga ctaaagataa
     7201 agtttatgat gggaatctaa ttctagtaaa tgctttttta ccagttaaaa catctgaaga
     7261 tatagattta attcctgtag atacaaggtt tccaagtatt cttatgaaac gtgaagctac
     7321 aaacatttta caaaatattt taaaaagtat atgtggaata aatgaaatag tacctgtaag
     7381 tggatatcgt actgcagagg aacaacaaga tatatattca agttctctgc gtgacaatgg
     7441 aaaagacttt actaaaaagt ttgtagcact tccaaatcac agtgaacatc agacagggct
     7501 tgctattgat ttagggttaa agaaagaagt tatagatttt atctgccctg aatttcctta
     7561 taatggtatt tgtaatgagt ttcgtaaggc agcactacat tatggtttta tagagcgtta
     7621 tcagtgtaaa aaagaagaaa taacaggaat atctcaagaa ccatggcatt ttcgttatgt
     7681 gggatatccg cattctgaga ttatcaataa aatgaatctc gctcttgaag aatatataga
     7741 atatattaag agattttcgt atacaaattg tttaaaaata gaagataaag aaaaaattat
     7801 agaaatcttt tatgtgccta ttcatactaa agaagaagtg agaatctcta tacctgaaca
     7861 atgtgtatat caaatatcgg gtaacaataa ggatggtttt attgtgacat tttggagaaa
     7921 tagaaaggac taagaaatgg aggaacaaga atgattagga ataaatctta tgcagggctt
     7981 gattatttta ggattatagc tgcattactt ataattgcaa ttcatacatc tccattatta
     8041 tcatatagta aaatggctga ctttgtgtta actagtataa ttgctcgtat tgctgtgcca
     8101 ttctttttta tgctatcagg cttttttcta atttcaacat ataattataa ttgtaataaa
     8161 ctgaaatctt ttctaaaaaa tgtattattt atttacggga tagctattgt aatttatatt
     8221 cccttaaata tttacactgg atatttttcc atgaataatt tagtgcccaa tattattaag
     8281 gacattgtat ttgatgggac tttatatcat ttatggtatc tacctgcatc aattattggt
     8341 gcaagtattg catactttct tgtaaaaaga ctaggtttaa agtactcact agtaatcact
     8401 attgttattt atgtaattgg tatgtttggt gatagttatt atggtttttt agaaaaaata
     8461 ccactattaa agaatttata tgggtatatt tttgaagtat cagattatac acgaaatggt
     8521 atattttttg cacctgtatt tttcatactt ggtggaataa tatctaataa atctaattat
     8581 atgtcaacta aaaatagctt gattggattt cttatatcat tcttacttat gttatgtgag
     8641 ggaatgtttt tacataaact aggggtacag cgtcatgata gcatgtatat tatgttattg
     8701 ccatgtatgt atttcttatt tacagcctta acatttttga aaggttctcg agctaggtta
     8761 ttgcgaacat catcattaat tatttatatt atacatccta tgatgattgt tgttattcgt
     8821 atgttttcta aaatattcgg aatacaaact gtcttggttg acaatagcct tattcattat
     8881 atagtggtaa gtatagcatc ttttatagct tctataatta ttatattgat tcttaaacct
     8941 ctaaaaaaag acttgaaagg agaacataag gtaaatacca atagagcttg gattgaaatt
     9001 gatttaaata atttggaaca taatgtaaag atgatacaaa aagctatgcc agaagattgt
     9061 gaattgatgg cagttgtaaa agctaatgcc tatggtcata gtgcttttga agtagcgact
     9121 tgtgcaaacc aaataggtgt aaaagcattt gcagtagcaa caatagatga ggggattgat
     9181 ttacgtcgat atggaatact aggagaaata ttgattcttg gatatactaa cccaataagg
     9241 gcaaaggaac ttcataaata caatcttaca cagagtgtta ttgaccttaa tcatgctatc
     9301 tctttaaaca aacagaaata caacataaaa tcacacatta aaattgatac aggtatgcac
     9361 agactgggtt ttgatgttaa tgatgtgagg tcaattttaa aggcatttaa attaaaatac
     9421 ttagatatat gtggcatatt tacacacttg tgtgtttcag atagtctgaa agatgaagat
     9481 gtagacttta caaataaaca aattgaatgt ttttataagt taattgattt tctgttaaaa
     9541 aaaggtataa aaattcctaa agtacatatt caaagtagtt atggtttttt taattatccc
     9601 aatcttaagt gtaattatgt aagaattgga attgcattat acgggatatt aagttctcca
     9661 aatgatacga ctaaattgca acttgattta cgacctgttt tatccttaaa atcacaagta
     9721 attcttatta gaaaaataca aaaagatgat agttttggat atggaagagt ctttattgca
     9781 agcagagata gcgtaattgc tatattatct attggctatg ccgatggtat acctagaaat
     9841 ttatcctgtg gaaaaagtta cgtaataata aatggttgta gagcagcaat agtgggtcga
     9901 atttgcatgg accaacttgc tgttgatatt acagatatac ctaatgtaga agttggtaat
     9961 acggcaataa taataggaag agataattta tctgaattat ctgcttctga agttgcaaat
    10021 aactcatgta gtatatcgaa tgaattattg agcagagtag gaagaaggtt aaatgttata
    10081 aagaagtaac tcaattaaat agtaatattt gttgtactgt gtaatttatt ttactctgac
    10141 tagacatttt tagtaatatt atgtctaata tatatttttt ataacttaca tgacaattta
    10201 agttataaaa aggtgatgtt tcagattttt attttgaaac atcacctttt ttgttataat
    10261 atataataaa ataattatac cttaactgta aacttatata gattatttac tttttaatat
    10321 tagattcatt ttttgagatt ggtatataat aaacatatag agtatataat aatagttaaa
    10381 gttaaaaaag gggaaaagca atatgcagta tatacaagaa ctacttgatt tgactcaaaa
    10441 ttattgggtg ttagccacaa taatcggctt attaagtgca tttatagaaa gctttatacc
    10501 agccttacca cttgttgcta tagttacagc aaatgctgca atacaaggat tatttattgg
    10561 atgtttgtta tcatggattg gctcaggact tgggacaact tcattattct tattaataag
    10621 cagatttaat gatagtaagc tttttaataa gttgagaaat agtaaaactg aaaaagcaat
    10681 aagttggatg gataaacagg gatttaagtt attatttata gcgtatgcct gtccatttat
    10741 gcccggttgt ttagttacta tcgcatcagc ttttggtaaa aaagatttaa aagattttgt
    10801 gccagcaatg ttggcaggaa aatttgtaat gtttatagtt ataagttacg tggcaagtga
    10861 tatagaaggt tttataacaa gtcctttaaa gatagcatca tttatattat tggtgttttt
    10921 gtcatggaag attggaaata gagtaaataa aaatcttgaa aatcataatt atgatttcca
    10981 tcataaaaaa catcacaatg ataaagatga taaaatgatt tagcttgtac gaaataaaac
    11041 gtatcttaaa atgatttagt actaaattat tttaagatac gttttatttt ttgtattaag
    11101 gtacaaaatt tttagttctt ttgtaaacta aaatatataa aacttattta tacttagttc
    11161 aaaatattgt tatttatttt ttctacaact ataataaaat ataaaattga tatccaatca
    11221 aaaaaataac tatgaaattt aatagcttag atatgaattt ttctgttttt gagttagttt
    11281 ttataggtag taaagaaatg tttatggtaa tgggataaaa caattcaata ccttctttag
    11341 taataaggtc aagacacaag tgagatagat atcctattat aaacccactg gacaaacatg
    11401 agaaaactat attattgtta gtgaagattg tcagagattt aaaaaaacta taaataagta
    11461 ataaggcaat tagactgtga gtaaaccctc tatgtctaaa cctacttcca aataatttgt
    11521 atattaacat aaatttttta cttatgtaag aacctctcat atctatatct ggaaataaag
    11581 aacctaaata tgcaaaataa atataaatta taattaatac tattttatat gcaatatcgt
    11641 atttaactag gtatagattg tttataaagg ggagtatcaa aagtgcgaat atataacctc
    11701 ccctttcatg tgtttctttt atcatataaa aatcctcctg aatcgtcact ataaacatta
    11761 tattggtaaa tttagactat cttttattat attatatagc aaacataagt ttttttcaaa
    11821 aggattttaa tatttaatta atcttgagat tttaaatttt tgtaattttc taaagcacca
    11881 ttccaattta ctacattaaa ccagttgtca atgtattcat ttcttctatt ttgatatttt
    11941 aaatagtaag catgctccca aacatcaagt cctattatag gagttaggtt tttacttaca
    12001 gggctatcct gatttggagt agtcataata gataatttgc catctttagt agctacaagc
    12061 caagcccaac cagaaccaaa gacatctaaa gcagattttt ggaactcttg cttaaatttt
    12121 tcaaaagaac caaagtctct atcaatagct tcttttaaag attcagaagg tatggttttt
    12181 tctggcgtca ttatatcaaa aaagaattta tgattataag ctccacctgc attatttctt
    12241 acagttgtag caatatcttt aggtaaagaa tctaaatttt gcaataattc acataaagaa
    12301 taattataaa gctcaggata tttttcaaga gcagcattta atttatcaac ataagcttga
    12361 taatgcttat catgatgcag tttcattgtt tctttatcta tataaggttc aagtgcatca
    12421 tatgcataag gtaatggttt aaccttaaat ttgttatttt caggtgtaaa agcaaatgaa
    12481 gttatgcact gtgagattat aaataaagac ataataactg gtattaatat ttttttcttc
    12541 ataaaaatca cctcctaata tagtatttct agaaataaaa aaaatatgaa atattaacag
    12601 ataatttata agaatttaat agcaatttca ttttaaattg aataagtttt aaagccaaag
    12661 acaaaactat gaaatataga ataagactaa aggacagaaa ggaattttga ttatatggac
    12721 ggcaatattt caatttatta taaaggtgat atatctaaaa aagaagatat taaatttaaa
    12781 ataaaagaat ctgtaaataa tacaagactt tctagtagaa tagagtataa ggatgaggaa
    12841 aaaacttttg attttgaaag atttaatgta attgatgcta tttatggaaa taaaccattt
    12901 gaaaaagatg gaaaagttat tgtttttaat ggaaatatat ataactatca tgaaataaaa
    12961 gaagaattaa ttaaaaaagg acacaatttt actacaaatg atgatattga agtcttatta
    13021 gcatcttata tagaatttgg taagaattgt gtacataaga ttaaaggaat gttcaacttt
    13081 ataatctatg atagagaaaa tgaaagtata tttggagcaa gagatttatt tgggataaaa
    13141 ccactttatt atataaataa ggaaaatgca ataatttttt cctcagagta taagttttta
    13201 cttgaatata tgaaaaattt aaatataaat gaaagaagct tacaaagtta tttttcattt
    13261 caatatgtac ttcctgaaga tactatgata caaggaataa ggctgatacc agcaggtcac
    13321 tattttaggg tagaaaatgg tatactatct ttaaagagat ataataaact tgagtttaga
    13381 agtagtacaa aattttttta tactaaaaat cacttaggca atagagatgt gaatgaggat
    13441 gatataagaa atatagtagt agattcaata gtaactcata tggaagagga gaaagaaatt
    13501 ggaacatttt tgtctggtgg aatagattca tctattataa ctactgtggc ttcacaaatt
    13561 aatccaaata taaaatcttt ttctgcaggc tttagtgtca aaggatatag tgaattagaa
    13621 gtagcaaaga aaacagctga taagttaggt atagaaaata tccagattaa tataacacaa
    13681 gatgaatata taaaatcatt accgaatgta atttattctt tggatgaccc tatagcagac
    13741 ccttcagaag taggtcttta tttcttaatt aaagaggcag gtaaacatgt taaagtagca
    13801 ttgtctggag aaggagcaga tgaattattt ggaggatata atatatataa agagtatagt
    13861 actatgaaat cagttgtcaa ttcacctact tatattaaaa gtatattagg aagagtttca
    13921 gaacttatgc caaacataaa aggaagaaat tatctttata gagctacaac accattagaa
    13981 aaaagatata taggaaatgc aaaagttttt gaaaacagcg aagtaaaaag attctttttt
    14041 aagtataagg aaaagaatat atatgaatac ctgttgtcta atttgtatag agatgcacaa
    14101 aaaaataatt acgattatat aagtaaaatg caacatatag atgtgaatac atggcttcaa
    14161 ggcgatatac ttcaaaaaat tagcaaactt tctacagctg agcaggttga acttagagta
    14221 ccatttttat ataaagatgt atttgatgtc gcaaaaaatt taagaatgga gcaaaaaata
    14281 aataagaata atacaaaagt tttacttaga gaggcgttta gagaaatagt cccagaacat
    14341 gttgttcaaa ggaaaaaatt aggatttcca actccaataa gagtttggtt aaaagattct
    14401 ctaggggata ttgtaaagga aactatttat aattcaaatg tagatgaatt tatagataaa
    14461 aaatatgcaa taaaactttt agatacgcat cttaaaggtc atagagataa ttcgagaaaa
    14521 atatggacta tatttacatt ttgtctatgg catcaattat ttattgagca taaaaatgtc
    14581 gagtattaat acttttttca atattaatca gagggcaaaa gcatgcaatg ttttcaccct
    14641 cttgattatt atggaatagg ataagtagaa ttttgattga ttagtttact atctcaataa
    14701 aattattggc aaatattttg acaagactac tttcaacagc taaatttaag ttttgtccat
    14761 catcatatcc agcggaaatc aatcctagta agtttccaaa catatctaaa agagctcctc
    14821 ctgaactacc actagagatt ggagctgtaa attgtatcat ttgaaccgtt tcaaaatcac
    14881 gaaaagctga aataattcca tctgaaactg tattaaaaag acctagaggg cttccaattg
    14941 caactatttt ttgacctctc acaggttttt tactgacttt aacaggtatt ggcttgcatt
    15001 ttctatcaac ctttatcaca gctaaatcat aatctgaatg gtattttata atttggtaag
    15061 atgtataaac tttatcatca ttttcaaatc taactaaaaa tgaataccca tcattgacta
    15121 catgaagatt cgtaagtata taaccttcat tatttattac tactccagaa ccgctcttaa
    15181 acggtttatt ttcgttattg cacacaacaa tcataacaac agaagctgat aactttgcaa
    15241 ggtcttcata agaaagttct acttcctcat cttgtacaat ttttttagat gttagatttg
    15301 tgttattaag tgtattttta gagttaggtt tattcgtagt aggagaagtc ttattggtat
    15361 tttcactgta atttatattt gtaatatgcc tttcaaaagg tatttctaat atatttggag
    15421 tcttcctaga aagttcaaat agagtagaaa attttgaaca ttcaggatat aaatcaagaa
    15481 tatctttatt tggatttata tataaaatat cacacccaag ttgtgataaa aaatataaga
    15541 aaaaaagttc ttgcctttta atatttccaa agtatactat ctttggtgaa atagtagtct
    15601 tcaccttgtt ataaaataat ttaggtagaa aatagtctat ccaacacaaa agtttaactg
    15661 caaagttttt gatgatagag gatgtacaat ttggcatact tgtggaaaat aaatctaaag
    15721 tatttttaaa tgcaatcttt ttagtccaat ttaaggtgtc attttctatt ttacatggaa
    15781 agtcgatatt atataaatcc caactgttaa acttagaact attataaata ttaaaaattt
    15841 taagcatctt atctgtttgt tctttgtcaa aatttatttt tagattgttt aatctaatat
    15901 agggaattgt aggattatta aaaaaataag tatctatatt tttaattttt tctatgtatt
    15961 tatcaattga atcatcaaca cctatatatc ttgtaaagta gacttcttta atatatgaag
    16021 aactcttatt tttataggta gtatcagata taaattcaat tagaggttca gtagactttt
    16081 ttattgaaat ctttaatggt ttaatagtac aaatcatatt ttttctccta taataagatg
    16141 atataatcta ttataaaaca acttaaaaca atatagtaca ataattttgt agtttcaaaa
    16201 taaaatatgt acttgtttat ataaatgata tataattaca ataaaattgc cgaaaagccc
    16261 tctcctttag gtgta
//
LOCUS       DAMJYR010000333        15199 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.guided.092, whole genome shotgun sequence.
ACCESSION   DAMJYR010000333 DAMJYR010000000
VERSION     DAMJYR010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15199)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15199)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15199
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.guided.092"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            278..1231
                     /gene="blaCDD"
                     /locus_tag="PC471_003984"
     CDS             278..1231
                     /gene="blaCDD"
                     /locus_tag="PC471_003984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDD family class D beta-lactamase"
                     /protein_id="HDF2791008.1"
                     /translation="MKRKKNFIWIAILLVGVVMVMYYVGKKHNDINQKTDKNYIKSEL
                     NKSELNKSKIENNNKNKKEKINMVDYSDCFEGISGGAIFYNTKNKEYNIYNKELIETR
                     RSPCSTFKIVSTLIGLEKGVINSKESVMGYDGTEYPNKNWNKNLSLEEAFKESCVWYY
                     KKLIDKVDAKSVQNILDDLKYGNCDISEWEGDLKNGKGHLNGFWLESSLQISPKEQVQ
                     TMAKIFEGDTNFKKEHINILRDIMKIDVNDKNINVYGKTGTGFDEKNKRVDAWFVGML
                     EREGDTYYFAIKSDDSNKEITGPKVKEIAINIIKKYYSVRE"
     gene            1575..2258
                     /locus_tag="PC471_003985"
     CDS             1575..2258
                     /locus_tag="PC471_003985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2791009.1"
                     /translation="MEILKCENLTKIYGSNQTRVTALNNVNLSVQKGDFVSIVGASGS
                     GKSTLLHLLGGVDRPTSGKIYVEDTEISSLKEEALAVFRRRKVGLIYQFYNLIPTLDV
                     RKNILLPMLLDKRKVDEDRFSEIVSILGLSDRLNHLPSQLSGGQQQRVSIARSLIYRP
                     AILLADEPTGNLDRKNSEEIVDLLNLSNKRFNQTILLITHDEKIALEANRIVTMEDGV
                     IVSEKVVKK"
     gene            2255..4915
                     /locus_tag="PC471_003986"
     CDS             2255..4915
                     /locus_tag="PC471_003986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2791010.1"
                     /translation="MNILQEYTLDFVRRNKRSSIAIMLAILLTTTMMSSLCGLLYTMW
                     TDFIKLSIEKEGNWHGELFDYTYGSDLPLIENFSSVDDVMVKGQWYVGKINDEKRDYI
                     IYRNATSEYWNSMPEKDTIIEGRRPNNANEIALSKQYFENNPSVKIGDKITLPIGDRI
                     LNGKPLEPIAPKSKGESFKKISEATLTIVGKVDVSTSSVIPAYTALGYLDKTSIKPND
                     SITVYLRFKNIRDTYKELPKLAKSLGWKTNEYGKYNLKYNSNYLLKMLVFSPEQKASM
                     SSISKFSTPIMYLTIAIFTVAVFVMVIYNAFSLSANARLTQLGILSSVGASPKQIKRS
                     VVFEGFLLTIIPLPIGLFLGWLLCNRLIVYVNSVNYHTDVPEVVFTYGIPAFLPAVLL
                     TIVTVWISALIPARKVSKISPIEAIRQGDSVKIKKSHKYSLGKIFGIEGELASNALRA
                     RKKSYRTATISLTLSFLLLTCFLHIIANQEGAKAVYGSDNYKDQRDIAVYLENSEPVE
                     SAFIEKLDNTEGVKSSLYYQKMSASTWLTEKDASKELAKNGGFKNIASSKKYSPIERD
                     GKFRIISNIIGLDDKSFTEYCRQISVDPKLFYDTKNPTSIVYNKEEDINRSTRRNKVY
                     IDYLNLSIGDNIKLNEKVYDEDKGDYTYNMKVGAITSTLPKIVDTSSKYTLMQIVPMS
                     MVQEISKNYDEVSRLRSNRLIGVFKVNNREDIPRVREKLESIYNKDYGSGDYSIEDVL
                     ESEKQKASGIKTMNLIVGFITGLLAMIGLSNVLATVSGNIRSRRQEFAMLRSVGLSPE
                     GIKKMLILEGLFLGITPLLLSIPVQIGIVYAFLRINEIYFIEYLPFAPISTIIGFTIL
                     ILFIVIASYMTGYKQLKNENIVESIKNETI"
     gene            complement(5096..6277)
                     /locus_tag="PC471_003987"
     CDS             complement(5096..6277)
                     /locus_tag="PC471_003987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF2791011.1"
                     /translation="MKTPRNINSTVVFIIFCLIVTFLLSVILTTITSFNEYNVLSKAL
                     GASYEYNPKSTESILKSLKNSSDKDFKTGNEILSKHGYSKSTFWSKNFFYFLVISFGI
                     ISTMATVVYIIKHRTNKQKLSRINNLTQYLEEVNLGKESVLLRCEDEFSHLEDEIYKT
                     VGELRCSKEFALKERQTLSDNLADIAHQLKTPITSMSLMAQLLSENCPDEEIDYINRL
                     TNQISRLERLVSSLLTLSKLDASTLIFESKLIDVHSLLTYAIEPIEGSLREKHQTFTI
                     EASPSVEFEIDINWTLEALLNILKNCSEHIEDGGYIVAYYSQNPLYVEIIIEDNGKGF
                     AQEELPYIFNRFYRGKNASKDSIGIGLALSKSIIERQNGTLHAENCHNGGARFIIKFY
                     T"
     gene            complement(6277..6939)
                     /locus_tag="PC471_003988"
     CDS             complement(6277..6939)
                     /locus_tag="PC471_003988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2791012.1"
                     /translation="MAKILLLEDNDTITFGIKTSLEKKNHTVCCYNTIAEAKKNFDKT
                     IDLILLDLNLPDGIGYEFCQYVKSIEDTPVIFLTVRDDEKDIIRGLDMGADDYVVKPF
                     LLSVLQSRINAVLRRTHSNTNNTITCENISLNKVEVKAYLDNVEVPLTAGEYKLLLSL
                     MENKNRAMTRTKLLEMLWDVDGNFVNDNTLTVTIKRLREKLKNPPCIKTLRGIGYRME
                     DN"
     gene            complement(7071..7709)
                     /locus_tag="PC471_003989"
     CDS             complement(7071..7709)
                     /locus_tag="PC471_003989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDF2791013.1"
                     /translation="MSELSKRDKEKIQRENEIIDKAEKLFCLNGFDNTTMNELAKEVE
                     YTKRTIYKYFSCKEDLFFAVVLRGYKRLWDNVKIESAKGKTGFEKIKLSYFAFHKFYS
                     VEPSLLCLMGMIGIVNSEKSDTDMLFKEKFFSFNKFMFDEIQGMFEIGKNDRSIRHDI
                     EIPTLMYSSIFTLTGFFNLLSVTGKSYLNNFNIDEEKFIETTLALLIDSIKA"
     gene            7888..8493
                     /locus_tag="PC471_003990"
     CDS             7888..8493
                     /locus_tag="PC471_003990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDF2791014.1"
                     /translation="MKIKENVLMLDSTRGSNCFIVFDKEIILIDTGLPFMGKNIIKEL
                     KTLGIKLTDIKHILLTHHDVDHISNIKLLKEKTGAKVWAHVEDIPFIIGEKDRPGFKK
                     FIGKAISRRLIKDVEPYGENMQIGNIKIIHTPGHTPGHVCMIFEDVLFAGDLVKNKNG
                     NIVPYPNPWNWDYKKMMKSIRELDNLKYEWICMSHGTPCIK"
     gene            8997..9746
                     /locus_tag="PC471_003991"
     CDS             8997..9746
                     /locus_tag="PC471_003991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDF2791015.1"
                     /translation="MRLESLVNQNYDNLNENDKIIIKQILICQREYRNFSCEKIANEC
                     NVSRATLLRLCRKIGLDSFSDLKYLLKNNQLEFVNNDNNFHDVCENYRLLMNELQKIS
                     FQDICKIIYDAETIYIYGTGNEQKSLAQEIKRIFLSVGKCVIDLFDIGEIEFMRESFQ
                     KSDLFIIISLSGETKAGIEIAKFINNHIHTISLTRLDNNTIATLCEYNLYVETQKLDT
                     LQAISYEVVGAFYALLDLLHMNYIEYRRRKE"
     gene            9743..10507
                     /locus_tag="PC471_003992"
     CDS             9743..10507
                     /locus_tag="PC471_003992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDF2791016.1"
                     /translation="MKIEGLFNKYYNSFTANDKYICECILNHKEDCIKLSIDEFADKY
                     HISTSALSRFAQKLQLPGYSELRVIIRLNETEVEYKNQSKHQLMDCYNKVINDIEKKD
                     CSIMFERMKKANRIIVFGEGYSQERVAKEMKRIFLPTGKIIYDVYGYDMIQALNHFIK
                     TDDMIFFISLNGDASTIIDFAKEMRMKGIYMVSITKMISNPLARLCDENLYIQSISIS
                     VDKQLDYDVTTPYFILIELLYIKYKMYLESEHIPRK"
     gene            10606..11427
                     /locus_tag="PC471_003993"
     CDS             10606..11427
                     /locus_tag="PC471_003993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDF2791017.1"
                     /translation="MKLYKLLILDIDGTLRDEVYGIPESAKHAIRLCQKNHCSVVICT
                     GRSMGTIQDDVLSLGVDGYIAGGGNYIQYHGELLYNQSFNQRLIKEVVCLLKNREVAF
                     SIESQEKVFMNQKAKEIFETMNQLKGTNSCINKQHIQEKITYENNIEEYKSQDIHKIC
                     LWSNEKVFDEVKDILQDKMELAQRDISSQYYEIIQKDFHKGKAIKRLQERLGVTQKET
                     ICFGDGQNDIVMFQASDVTIAMKNSHQQLKDIATSICEDIFDNGIYKELKRRNII"
     gene            11441..13105
                     /locus_tag="PC471_003994"
     CDS             11441..13105
                     /locus_tag="PC471_003994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HDF2791018.1"
                     /translation="MEKKWWQKEIVYQIYPRSFQDSNNDGIGDLQGIISRLDYLKDLG
                     ITSLWLCPIFKSPMDDNGYDISDYLDIASEFGSMEDLKELIKKSKDRGIKIILDLVLN
                     HTSDEHPWFQEALKNPESPYHDYYIFKKGNDLPNNWRSVFGGSVWEKVENRDEYYFHS
                     FGKKQPDLNWENPILRSELYQMINTWSQMGVAGFRVDAITFIKKNLSFQSLESDGVDG
                     LVKCTKTVRNQPGIEKMLYELKENTFKKYDCMTVAEAAGISYDQLGDFIGEDGFFSMV
                     FDFKHADLDVASGSEWFKRISWTIPDLRKCILDSQMYVQEHGWAANFIENHDQPRATT
                     KYLLQYERNDDAVKMLGAMYFFLRGTPFIYQGQELGMTNFKRDTINDFNDLSSIDQYY
                     RSIDEGYSEEEALEFVNLRSRDNARTPFPWADKENAGFSDTTPWLKVIENYKDINAQS
                     QINNSESILEFYKKMIKLRQDSEYSDCLIYGSIEPIDIESDDIIAYRRQLDLVVIDCY
                     YNFSDKPVEIRVEEKYDIVFQNLDDINLLNKTVRLKPFHAVLLKVR"
     gene            13110..14984
                     /locus_tag="PC471_003995"
     CDS             13110..14984
                     /locus_tag="PC471_003995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDF2791019.1"
                     /translation="MNKYNKIANELIKIIGEDNIISITHCATRLRVMVKDREIINDKK
                     VEKVDEVKGVFFTSGQYQIILGTGIVNKVYAEVEKMGLKTLSKKEQDELVKNNETGFK
                     KVMRTLADIFVPIIPVIAATGLFLGLKGCLFNDNVLGLFGMSSANIPLYIQTLVSVLT
                     ETAFAFLPAIIVWSAFKVFGGTPVIGLVIGLMLVSPILPNAYSVADPSNEIEAIMAFG
                     FIPIVGCQGSVLTAIVTAFIGANLEKWFRKHMPNVLDLIFTPFFVMLITMLVILLGVG
                     PIMHTIELKMVDIISLLIDLPLGIGGFIIGFTYPLAVITGLHHTYVMIETSLLANTGF
                     NALITLCAMYGFANIGTCLAFMKKSKNNQVKQTAVGAMLSQLFGISEPVLFGIQLRYN
                     LKPLIIMCASSGLGAAILSILHIQSNSYGLAVLPSYLMYIYDGYNLITYLLASIFVVA
                     FCFIVTCLFGVPKEAINEDEDEELVFNENNENFVSPAKGKIVALENVPDETFSKKMLG
                     DGFAIDIIDGKIVSPISGKLETVVSSGHAFGIKGTNGEVLIHVGIDTVALNGDGFDIA
                     VKQGDMVKQGDVLVNVDLKRIHELGKSTLTMVLFPDGKKVNILDINKDVKIGQRICVE
                     "
BASE COUNT         5709 a         1744 c         2526 g         5220 t
ORIGIN      
        1 attgtaatat ttaaatatta caatagtaag ttttaaatgt ggaatcaatt attatatcac
       61 aaattaaaag gaggacagga tgttattaat agttacttaa ttatcttcgc aataggttta
      121 atcaacataa ttgtactagg tgaagaaaat ataagagata cagctattca tttagtcttg
      181 tttcttataa gtagaaccta gattggagta tattacaaat aatgtctact attttaatag
      241 taggttataa atttaaatat gggagatgat ataaattatg aaaagaaaaa agaattttat
      301 ttggatagct atcttattgg ttggtgtagt tatggtaatg tattatgtag ggaaaaaaca
      361 taacgatata aatcaaaaaa ctgataagaa ttatataaaa tctgaattaa ataagtctga
      421 attaaataaa tccaaaattg aaaacaataa taaaaacaaa aaagaaaaaa ttaatatggt
      481 tgactatagt gattgttttg aaggtattag tggaggagct attttttata atactaaaaa
      541 taaagaatat aatatatata acaaagaatt aattgaaaca agaaggtcac catgttctac
      601 ttttaaaata gtttcaactt taattggttt agaaaaaggg gtaataaact caaaagaatc
      661 agttatggga tatgatggta cagagtatcc aaataaaaat tggaataaaa atcttagttt
      721 agaagaggca tttaaggagt cttgtgtttg gtactacaaa aaattgatag ataaagttga
      781 tgctaaatct gtccaaaaca ttttagatga tttaaagtat gggaattgcg atattagcga
      841 atgggaagga gatttaaaaa atggaaaggg acatttaaat ggattttggt tagaatcttc
      901 attacaaata tctccaaaag aacaagtgca aacaatggca aaaatatttg agggagatac
      961 aaactttaaa aaagagcata ttaatatttt aagagatatt atgaaaatag atgtaaatga
     1021 taaaaacata aatgtatatg gaaaaactgg aacaggattt gatgaaaaaa ataaacgtgt
     1081 ggatgcatgg tttgttggta tgctagagcg tgaaggtgac acctattatt ttgctattaa
     1141 atcagatgat tccaataaag aaattacagg gccaaaggta aaggaaattg caattaatat
     1201 aattaagaaa tattatagtg taagggaata aacttaattt tatttgtaaa tattatttac
     1261 ctatagtctg taatatttat taaataattt tactaaattg agtctatata gagcaaaact
     1321 cctttagtac ataaaatact tgctattgat tattaaattg aatacctata gatttaaagt
     1381 taataccttt taaagactat gtaatatatt aaaagtataa ataaaatata ttgcatagtt
     1441 tttgttttat attataattt taaataatgt gcaaaaacta aacaatattt ttaatagtaa
     1501 tttatatttg tattcatgtc actataatgt cattttaggt tgctaaaatt taaccataaa
     1561 ggagatgatt gtatatggaa atattaaaat gtgaaaatct cacaaaaata tatggttcaa
     1621 atcaaaccag agtgacagcc cttaataatg taaatttatc agttcaaaaa ggtgattttg
     1681 tatccattgt aggagcatct ggttctggca aatctacatt gttacatctg cttgggggag
     1741 tagatagacc tacatctgga aagatttatg ttgaagatac agaaatatca agtttaaagg
     1801 aagaagctct tgcagttttt agacgtagaa aagtaggtct tatttatcaa ttttataatc
     1861 ttatacctac ccttgatgtt agaaaaaata tattattacc catgttactt gataaaagaa
     1921 aggtggatga ggatagattc tctgagattg tatctatatt gggattatct gatagattaa
     1981 accatcttcc aagtcaactt tcaggtggac aacaacaaag ggtatctata gctagaagtt
     2041 taatctatag accagctatt ttattagcag atgagccaac tggtaatctt gatagaaaaa
     2101 attcagaaga aattgtagac ttgttaaact tatcaaataa acgatttaat cagacaatac
     2161 ttcttattac tcatgatgag aaaatagcac tagaggcaaa tcgtattgta actatggaag
     2221 atggagtgat tgtctctgaa aaggtggtga agaaatgaat attcttcaag aatacacctt
     2281 agactttgta cgacgcaata aacgtagtag tatagctata atgttagcta tattacttac
     2341 tacaaccatg atgtcttctt tgtgtggact gttgtatacc atgtggacag attttataaa
     2401 actttcaatt gagaaagaag gtaactggca tggtgaattg tttgactata cttatggaag
     2461 tgacttacca ttaattgaaa atttttcttc agttgatgat gtaatggtaa aaggacaatg
     2521 gtatgtaggc aaaattaatg atgagaaaag agattatata atttatagaa atgctacatc
     2581 agagtattgg aattctatgc cagaaaagga tactataata gagggtcgtc gaccaaataa
     2641 tgctaatgaa attgcattat caaaacaata ttttgagaat aatccaagtg taaaaattgg
     2701 tgataaaatt actctcccaa taggagatag aatcctaaat ggaaagcctc ttgaacccat
     2761 agccccaaaa tcgaaagggg aaagttttaa aaaaatttca gaggctacat tgacaatagt
     2821 aggaaaggta gatgtttcta cttcatcagt aatacctgca tatacagctc ttggatatct
     2881 tgataaaaca agtataaaac ctaatgatag tataactgtg tacttgcgat ttaaaaatat
     2941 aagagacacg tataaggaac ttccaaaact tgctaaatca ttgggatgga agactaatga
     3001 atatggaaaa tacaatctta aatacaatag taattatctt ttaaagatgt tagtcttttc
     3061 accagaacag aaagcatcta tgagtagtat atcaaaattt tcaactccta ttatgtatct
     3121 tacaatagca atttttacag tagccgtgtt tgtaatggtg atttacaacg cgttttcact
     3181 gtctgcaaat gctagactta ctcaattagg gatattatct agtgttggtg catcaccaaa
     3241 acaaattaaa aggtctgtag tgtttgaagg ctttttactg acaattatac cattaccaat
     3301 aggtttattt ttaggatggt tattgtgtaa taggcttata gtttatgtta attcagttaa
     3361 ttatcatact gatgttccag aagtggtatt tacttatggt attcctgctt ttctgccagc
     3421 agttttactt acaatagtaa cagtctggat ttctgcactt ataccagcac gaaaagtatc
     3481 aaagatatct cctatagaag caatacgaca aggagatagt gttaaaatca aaaagtcgca
     3541 taaatattca ttaggaaaga tatttggaat agaaggagaa cttgcatcaa acgcactacg
     3601 tgctcgtaaa aaatcttatc gtactgcaac aatatcttta acattatctt ttttattact
     3661 tacatgtttt ttgcatataa ttgcaaatca agaaggagca aaagctgttt atggaagtga
     3721 taattataag gaccaaagag atatagcagt ttatttggaa aatagtgaac ctgtagagtc
     3781 tgctttcatt gaaaaattag ataatacaga aggtgtaaaa agttcactat attatcaaaa
     3841 aatgtctgct tccacttggt tgactgaaaa agatgcttca aaagaacttg cgaaaaatgg
     3901 aggatttaaa aatatagctt catctaaaaa gtactctcct atagaacgtg atggcaagtt
     3961 tcgtataatt tcaaatataa ttgggcttga tgataagagt tttacagaat attgtaggca
     4021 aatttctgta gacccaaagt tattttatga tacaaaaaat ccaacctcaa ttgtatataa
     4081 taaggaggaa gatattaatc gtagtacacg tagaaataaa gtatatatag attatttaaa
     4141 tctttcaata ggagataaca ttaaattaaa tgaaaaagta tatgacgaag ataaagggga
     4201 ttatacttac aacatgaaag tgggagctat tacaagcaca cttcctaaaa tagtagatac
     4261 tagttcaaag tatacattaa tgcaaatagt tccaatgtct atggtgcagg aaatatctaa
     4321 aaattatgat gaagtaagta ggttacgttc aaataggctg ataggagtat ttaaagtaaa
     4381 taatcgtgag gacattccta gagtaagaga aaaattagag tccatttata ataaagacta
     4441 tggttcagga gattatagta ttgaggatgt tttagaaagt gaaaaacaaa aggctagtgg
     4501 aataaagacc atgaatttaa tagtaggctt tattacaggt ttattggcta tgattggatt
     4561 atcaaatgta cttgctacag tgtcaggaaa tattagaagt agacgacaag agtttgctat
     4621 gttgcgttct gttggattat ctccagaagg cataaaaaag atgcttattt tggaaggttt
     4681 attcttagga attacaccat tactacttag tattcctgtg caaataggaa tagtgtatgc
     4741 ttttctacgt ataaatgaaa tatattttat agagtattta ccatttgcac ctatatctac
     4801 aataataggt tttactatat tgatactttt tattgtaata gcatcatata tgacaggata
     4861 caagcagttg aagaatgaaa atatagtaga atctataaaa aatgaaacaa tataaacaaa
     4921 atctatttga acttatttaa aggtttcgat ttaagactac ttacaagtat agggatagtt
     4981 aagttgtttg tttaataaaa ccttaaatgt aaatagaagt atattactaa aacaatagat
     5041 aacatagaat atagatatag ctggattata ggtattttac ctataatcca gcattttatg
     5101 tgtaaaattt aattatgaat cttgcaccac cattatggca attttctgca tgtagagtac
     5161 cattttgacg ttctataata gactttgata gagcaagccc tataccaata ctgtccttac
     5221 tagcattttt accacgataa aatctattaa aaatatatgg aagttcttcc tgagcaaacc
     5281 ccttaccatt atcttctatg ataatctcaa catatagagg attttgacta taataagcaa
     5341 ctatatatcc accatcttct atatgctcac tacaattttt aagaatattt aaaagagctt
     5401 ctaaagtcca attaatatcg atttcaaatt ctaccgaagg agaagcctcg atagtaaatg
     5461 tctgatgttt ttctcttaaa gaaccttcta taggctcaat tgcataagta agaagagaat
     5521 gtacatctat aagtttgctt tcaaatatta aagtacttgc atccaatttt gataatgtaa
     5581 gtaaagatga aactagtctc tcaagtcttg agatttgatt tgtaagtctg ttaatataat
     5641 ctatttcttc atctggacaa ttttctgaaa gtaattgtgc cataagtgac atagaggtaa
     5701 ttggagtctt aagttgatga gcaatatctg caaggttatc tgaaagagtt tgtctttctt
     5761 ttaaagcaaa ttcttttgaa caacgaagtt ccccaactgt tttatatatc tcatcttcaa
     5821 ggtgagaaaa ttcatcttca catcgtaaaa gtacagactc ttttcctaaa ttcacttctt
     5881 ctaagtattg agtaagattg tttattctac tcagtttttg cttatttgtt ctatgtttga
     5941 ttatatatac aactgtagcc attgttgaaa ttataccaaa cgatatcact agaaaataaa
     6001 aaaagttttt actccaaaat gtacttttag aatatccatg ttttgataaa atttcatttc
     6061 ctgttttaaa atccttatca ctagaatttt tcagtgattt tagtatactt tctgtagact
     6121 taggattgta ctcatatgat gcaccaagag ccttagacag tacattgtat tcattaaaag
     6181 aagttatagt tgttagaatt acactcaata aaaaagttac aattaagcag aatattataa
     6241 aaacaactgt actgttgata tttcttggag ttttcactag ttgtcctcca ttctatatcc
     6301 tatgcctctt aatgttttta tacaaggtgg attttttaat ttttctctaa gacgttttat
     6361 tgtaacagta agagtgttat catttacgaa attaccatct acatcccaga gcatttctag
     6421 taactttgta cgagtcatag ctctattttt attttccata agacttagaa gaagtttata
     6481 ttctcctgct gtcaaaggta cttcaacatt atctaaatat gctttgactt caaccttatt
     6541 caagcttata ttttcacatg taatagtatt gttagtattt gaatgagttc ttctaagaac
     6601 tgcattaatt ctggattgaa gtactgagag caaaaacggt ttaaccacat agtcatctgc
     6661 acccatatct aatccacgta taatgtcttt ttcatcatca cggactgtca aaaatatcac
     6721 tggagtatct tctatacttt taacatattg gcaaaactcg tatccaatac catcaggtag
     6781 atttaaatca agcagaatta aatcaatagt tttatcaaaa ttttttttag cttcagcaat
     6841 cgtattataa caacatacag tgtgattttt cttttcaagg gaagttttta ttccaaatgt
     6901 aattgtatca ttatcttcta aaagaagtat tttagccatt tacttttaat cctttcagta
     6961 atttagttat tccattaact aagttattag ttttatgttt aatatttttc atattagaga
     7021 tagaaaaaat tatcatttaa gtatataaac tttaatttat aaacttaggt ttatgcttta
     7081 attgaatcta ttaaaagagc aagagttgtt tcaataaatt tttcctcatc tatattaaag
     7141 ttgttcaggt atgattttcc tgttacagat agtaaattaa aaaatcctgt aagtgtaaat
     7201 attgatgaat acataagagt aggtatttca atatcatgcc taatacttct atcatttttt
     7261 ccaatttcaa acattccttg tatttcatca aacataaatt tattaaaaga aaaaaacttt
     7321 tctttaaaca gcatatcagt atccgatttt tcagagttta caataccaat cattcccata
     7381 aggcataata gagaaggttc tacagagtaa aatttatgaa aagcaaagta tgaaagtttt
     7441 attttttcaa accctgtttt gccttttgca ctttctattt taacattatc ccaaagtctt
     7501 ttatatcctc ttaagacaac agcaaagaat aaatcttctt tacatgaaaa gtacttgtat
     7561 atggtgcgtt tagtatattc aacttctttt gctaattcat tcatagttgt attatcaaat
     7621 ccattaagac aaaataattt ttctgcctta tcaataatct cattttctct ttgtattttt
     7681 tctttatctc tttttgataa ttctgacata taaattattc ctttctattg ataaaaaagt
     7741 atactgatgg tatatttatg taaatataat atttgtatac ctatagtata ctttaataaa
     7801 aaataaaatt aatatcaagt attataacta tttataactt tattaattaa ttatataggt
     7861 aaaataccaa ataaggggaa tttatttatg aagataaaag aaaatgtatt aatgttagat
     7921 agtactcgtg gctcaaattg ttttattgtg tttgataaag aaattattct tatagacaca
     7981 ggtcttcctt ttatggggaa aaatataatt aaagaattaa aaacacttgg cattaaactt
     8041 acagatatta agcatatttt attaactcat catgatgttg accatatatc taatattaaa
     8101 ttattgaaag aaaaaacagg agcaaaagta tgggctcatg tagaggatat accttttatt
     8161 ataggagaga aggatagacc tgggtttaag aagtttattg gaaaggcaat ttccagaagg
     8221 ctcattaagg atgttgaacc ttatggagag aatatgcaga ttgggaatat aaaaattatc
     8281 catactcctg gtcatactcc agggcatgtc tgcatgattt ttgaagatgt attatttgca
     8341 ggtgatttag tcaaaaataa gaatggaaat atagttcctt atcctaatcc atggaattgg
     8401 gattataaaa agatgatgaa atctataaga gaattggata atttaaaata tgaatggata
     8461 tgtatgtctc atggaactcc ttgtattaaa taataaaaat taacatggat taatagaggc
     8521 gtagttgctt gtatgtatga taataattta cagcttcttt ttaatatatc atgtatattt
     8581 gtagctgtaa gcatatttat aacaatactt acaattttgt attttaacat ctataataat
     8641 aattttagct ttgttaacta tgaaatttac ttaaagaagt aaatattcaa gtagtagtaa
     8701 ttattaaata agataaaaaa taaatggcag atgaattaaa gtaatttcta ccatggaaaa
     8761 tacttttgag ttagaaaaaa ctctatttta gagtatgaag agatgaagct tccgaagaaa
     8821 gcaatatcta aagtacatgt tcgaaaggat aagtactttt tggtgcctgc ttttagcagg
     8881 ttatttttat tttaataaaa ataaaatcaa atgattattt tatccttatt atttaaaact
     8941 cttccctaga aaaagcaact aatgtatatt aaaataatat ttagggagtg gaaaaaatga
     9001 gattagaatc gttagttaac caaaattatg ataatttaaa tgaaaatgat aaaattatta
     9061 ttaaacaaat attgatttgt caaagagaat accgtaattt ttcgtgtgaa aaaattgcaa
     9121 atgaatgtaa tgtttctaga gctactttat taaggctttg tagaaaaatt ggattagatt
     9181 cattctctga tttaaagtat ttacttaaaa ataatcaact agaatttgtt aataatgata
     9241 ataattttca tgatgtttgc gagaattatc gactattaat gaatgaatta caaaaaatat
     9301 catttcaaga tatttgtaaa ataatatatg atgcagaaac aatttatatt tatgggacag
     9361 gtaatgaaca gaaaagttta gctcaagaaa taaagagaat atttctttca gtaggtaaat
     9421 gtgttattga cttatttgat ataggtgaaa ttgaatttat gagagaatca tttcaaaaaa
     9481 gtgatttatt tattatcatt tctttgtctg gagaaacaaa agcaggaatt gaaattgcaa
     9541 aatttatcaa caatcatata catacgattt ctttaactag gcttgataat aatacaattg
     9601 caacactttg tgagtataat ctgtatgttg aaactcaaaa acttgataca ttacaagcta
     9661 tatcttatga agtcgtagga gcattttatg ctctattaga tttgcttcat atgaattaca
     9721 tagagtatag aaggagaaaa gaatgaaaat tgaaggactt ttcaataaat attataattc
     9781 atttacagca aatgataagt atatctgtga gtgtattctt aatcataaag aagattgtat
     9841 taaactttct atagatgaat ttgcagacaa ataccatata tctacaagtg cactttctcg
     9901 ttttgctcaa aaactacaat tacctggata tagtgaatta agagttatta tacgcttaaa
     9961 tgaaacagaa gtagaatata aaaatcaaag taaacaccaa ttaatggatt gctataataa
    10021 ggtgataaat gatatagaga agaaggattg ttcgattatg tttgaaagaa tgaaaaaggc
    10081 aaatcgtata atcgtctttg gagaaggtta ttctcaggaa agagttgcta aagagatgaa
    10141 aaggattttc ttaccaacag ggaaaattat atatgatgtt tatggttatg atatgattca
    10201 ggcacttaat cattttataa aaacagatga tatgattttc tttatttcat tgaatggaga
    10261 tgcatctaca attattgatt ttgctaaaga aatgagaatg aaaggaattt atatggtttc
    10321 tattacaaaa atgataagta atcctttagc aagattatgt gatgaaaacc tgtatattca
    10381 atctatatct atttctgtag ataaacaact ggactacgat gtaacaacac cttattttat
    10441 tttaattgaa ctgctttata ttaaatacaa gatgtattta gaaagtgaac atattccccg
    10501 aaaatgaaaa tttgtgctaa aaatgaacat attttttaaa agatatcaaa atattgatat
    10561 cttttttttt gaatgctatt atgattttga tagtaagaaa ggaagatgaa attgtacaaa
    10621 ttattaatac ttgatataga tggtacgctt agagatgagg tatatggaat cccagaatct
    10681 gcaaagcatg ctatacgttt atgtcaaaag aatcattgca gtgttgttat ttgtactgga
    10741 agaagtatgg gtacaataca agatgatgtt ttatcattag gagttgatgg atatattgca
    10801 ggaggaggta attacattca atatcatggt gaattacttt ataatcaatc ttttaatcaa
    10861 agattgataa aggaagttgt ttgtttatta aaaaatagag aagttgcttt ttcgattgag
    10921 agtcaggaaa aagttttcat gaatcaaaaa gccaaagaaa tatttgaaac aatgaatcag
    10981 ttgaaaggaa ctaattcttg tataaataaa caacatatcc aagaaaaaat aacatatgaa
    11041 aataacattg aagagtataa gtcacaagat atacataaaa tttgtttatg gtcaaatgaa
    11101 aaggtatttg acgaagtgaa agatatacta caagataaaa tggaattagc acagagagat
    11161 atttctagtc agtattatga aattattcaa aaagattttc ataagggcaa agcaattaag
    11221 agattacaag aaagattagg agtaactcaa aaagaaacca tatgttttgg tgatggtcaa
    11281 aatgacattg tcatgtttca agcatcagac gttacaattg caatgaaaaa tagtcatcaa
    11341 caacttaaag atatagcgac atctatatgt gaggacatat tcgataatgg aatttataaa
    11401 gaattaaaaa gaagaaatat catataaaaa ggagtaattt atggaaaaga aatggtggca
    11461 aaaagaaatc gtttatcaaa tttatccaag aagctttcaa gatagtaata atgatggaat
    11521 tggagattta cagggaatta tttctcgttt agattattta aaagatttag gtattacatc
    11581 attgtggtta tgtccaattt ttaaatcacc tatggatgat aatgggtatg atattagtga
    11641 ttatttggat attgcaagtg aatttggaag tatggaagat ttaaaagagt taattaaaaa
    11701 aagtaaagat agaggaatca aaattatatt agatttagtt cttaatcata caagtgatga
    11761 acatccttgg tttcaagaag cattaaaaaa tccagaaagt ccttatcatg attattatat
    11821 ttttaaaaaa ggaaacgatt tgccaaataa ctggaggtct gtttttggtg gaagtgtttg
    11881 ggaaaaggta gagaatagag acgaatatta ttttcattct tttggaaaaa aacaacctga
    11941 ccttaattgg gaaaatccaa tattaagaag tgaactttac caaatgatta atacctggtc
    12001 tcaaatggga gtggcaggat ttagagttga tgctattaca ttcatcaaaa agaatttaag
    12061 ttttcaaagt ttagaaagtg atggtgttga tggtcttgtt aaatgtacaa aaacagttag
    12121 aaatcagcct ggaatagaaa aaatgcttta tgaattaaaa gaaaatacgt ttaaaaaata
    12181 tgattgtatg actgttgctg aagcagcagg tattagctat gaccagttag gtgattttat
    12241 tggagaagat ggtttctttt caatggtctt tgactttaaa catgctgact tagatgttgc
    12301 ttctggtagt gagtggttta aaagaatttc atggacaatt ccagatttaa gaaaatgcat
    12361 tttagatagt caaatgtatg ttcaggaaca tggatgggca gctaatttta ttgaaaatca
    12421 tgaccaacca agagctacaa caaagtatct tttgcaatac gaaaggaatg atgatgcagt
    12481 aaaaatgttg ggagcaatgt atttcttttt aagaggaacc ccatttattt atcagggaca
    12541 ggaattagga atgacaaatt ttaaaagaga taccataaat gattttaatg atttatctag
    12601 cattgaccaa tactatcgtt caatagatga aggttactca gaagaagaag cgttagaatt
    12661 tgtgaattta agaagccgag ataatgcaag aacgccattt ccatgggcag ataaagaaaa
    12721 tgcaggtttt tcagatacaa caccatggtt aaaggttatt gaaaattata aagatattaa
    12781 tgcacagagt cagattaaca attcagagag tattttagaa ttttataaaa agatgataaa
    12841 gttgagacaa gatagtgagt attctgattg tttgatttat ggttcaatag aacctattga
    12901 tatagaaagt gatgatatta ttgcgtatag aaggcaatta gatttggtag ttattgattg
    12961 ttattataat ttttctgata aacctgtaga aataagagta gaagaaaaat atgatattgt
    13021 attccaaaat ttagatgata taaatctatt aaataaaaca gtgcgtttga aaccatttca
    13081 tgcagtatta ttgaaagtga ggtagaaaaa tgaataaata taataaaata gcaaatgagc
    13141 ttattaaaat tattggtgaa gataatatta tttctattac acattgtgcc acaagattaa
    13201 gagtgatggt taaagataga gaaattatta atgataagaa agttgaaaaa gttgatgagg
    13261 ttaaaggggt tttctttact tctggacagt atcaaattat tttaggaaca ggaatagtta
    13321 ataaagttta tgcagaagtt gaaaaaatgg gattaaaaac tttatcaaaa aaagaacaag
    13381 atgaattagt taaaaataat gaaactggat ttaagaaagt tatgagaact ctagcagata
    13441 tatttgttcc tattattcca gttattgcag caacaggatt attcttggga ttaaagggtt
    13501 gtttgtttaa tgataatgta cttggattat ttggaatgtc ttctgcaaat attccattgt
    13561 atattcagac acttgtttcg gttcttacag aaacagcatt tgctttttta ccagcaatta
    13621 ttgtatggtc agcatttaaa gtatttggag gaacacctgt tattggactt gttattggac
    13681 ttatgttagt ttccccaata cttcctaatg catattctgt tgctgaccca tcaaatgaaa
    13741 ttgaagcaat tatggcattt ggatttattc ctattgtagg ttgtcaagga agtgttttaa
    13801 cagctattgt tacagcattt attggagcta atcttgaaaa atggttccgt aaacatatgc
    13861 ctaatgtatt agaccttatt ttcacaccat tttttgttat gctaataaca atgttagtta
    13921 ttttgttagg tgtaggccct ataatgcata caatagaatt aaaaatggtt gatatcatca
    13981 gcttattgat tgatttgcct ttaggaattg gaggatttat tattggattt acatatccat
    14041 tagctgttat tactggctta catcatactt atgtaatgat tgaaacatca ctattagcaa
    14101 atacaggatt taatgccctt attactcttt gtgcaatgta tggatttgcg aatattggaa
    14161 catgtctagc atttatgaaa aaatctaaaa ataatcaagt taaacaaact gcagttggag
    14221 caatgctatc tcaacttttt ggtataagtg agcctgtttt atttgggatt cagcttcgat
    14281 ataatttaaa gcctttgatt attatgtgtg catcttcagg tttaggagca gcaatattat
    14341 caattttaca tattcaatct aattcatatg gattagcagt tttaccttct tatttaatgt
    14401 atatctatga tggatataat ttaatcacat acttattagc atcaatattt gtggttgcat
    14461 tctgctttat tgtaacatgt ttatttggag tgcctaaaga agctataaat gaagatgaag
    14521 acgaagaatt agtgtttaat gaaaataatg aaaactttgt gtctccagct aaggggaaaa
    14581 ttgttgcatt agaaaatgta ccagatgaaa cattttctaa aaaaatgtta ggtgatggtt
    14641 ttgctattga tattatagat ggaaaaattg tttctcctat ttcaggtaaa ttagaaacag
    14701 tggtttcaag tggacatgca tttgggatta aaggaactaa tggagaagta ttaattcatg
    14761 ttggaattga tacagttgca ttaaatggag atggctttga tattgctgtt aaacaaggag
    14821 atatggttaa acagggagat gttcttgtta atgttgattt aaagagaata catgaattag
    14881 gaaagtctac tttaacaatg gtattgtttc cagatggtaa gaaggtaaat attttggata
    14941 taaacaagga tgtaaaaata ggtcaaagaa tatgtgttga gtagtaggtg atatgtaatg
    15001 actacaaaag aagattatca agagtaattc aatcttttta aagtatgaga aataattcat
    15061 aaactctaaa acaattaaag aggttgtttc aaaatagaga ttgtattgca cccaaattgt
    15121 tgaaatgatg aaaatatatt taacttcata caaaaaatta aatagtggca acaattgagg
    15181 atgtgaggga gagttgcca
//
LOCUS       DAMJYR010000334        12086 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.guided.118, whole genome shotgun sequence.
ACCESSION   DAMJYR010000334 DAMJYR010000000
VERSION     DAMJYR010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12086)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12086)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12086
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.guided.118"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            158..1564
                     /locus_tag="PC471_003996"
     CDS             158..1564
                     /locus_tag="PC471_003996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HDF2791020.1"
                     /translation="MNTGFPKDFLWGASSSAFQVEGAWDKDNKGKTVADYNSFKKSHL
                     QADTKVASDFYHNYEEDIELMKELGMKTYRFSISWARIIPDGEGEINQKGLEFYNKII
                     DKLIECDIEPFVTLYHFDLPFKLVEKYNGWESRETVYAFERFAKICFKHFGDRVKYWQ
                     PHNEQNLIVRVEERINIYDETDSWKIDKMRAQMDYNLCLAHALAVNACHEMIKESKIG
                     AAVSSSVTYPLTSKPEDVYAARMNDNFKVYYMLDMHHYGEYPGYYMKYLEKRNIVPHM
                     EDGDKEILKKAKMDFIAVNYYRTNCAEALPEDSQHPFGLREGTVDFSMYGLFKMSMNP
                     NLEASEYGAAIDPSGLRVALNEYWQRYHLPVIITENGLGAKDILEDGKIHDDYRIDYL
                     RSHINACKLAIEDGVEMIGYCPWSFTDLLSSSQGFNKRYGLVYINRTDHEVLDLKRIK
                     KDSFYWYKEVIENNGIVK"
     gene            1592..2440
                     /locus_tag="PC471_003997"
     CDS             1592..2440
                     /locus_tag="PC471_003997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDF2791021.1"
                     /translation="MYITKVLNNSLLLAKDDNDEEIILMGKGIGHNYKAGSELNKKDI
                     EKIFVLHDENIKKSIIQLATEIDAEYFGIAQMIIAYGIEKYNLKLMNHIYLALTDHIA
                     FAVRRFKEGIMIENHYLFEIKEFNPKEYDIGKYAITVFKEVLGLDLPEEEIGNIAGHF
                     INAQQDNPYSDRNKRSAKIVNAILQIVHYHFSIVYNKESFYYRRFVMHLKAFSQRFLA
                     NEPSREKIDFIYEQVQKNCKEEYECVKKVGAYMYKEYSRELPRQEELYLMIHIHKILG
                     ELCENS"
     gene            3101..3793
                     /locus_tag="PC471_003998"
     CDS             3101..3793
                     /locus_tag="PC471_003998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003020373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2791022.1"
                     /translation="MNKVLIIDDDKDLCILLKKSISIEQIEANYCHSGRDGLVELSKQ
                     DYQLVVLDVMMPGMDGFETMEQIRKYSSIPILMLTSKSDNYSKVHGLRAGADDYLTKP
                     FEITEFTARVLSLIRRYTRFNPKNEEPQQLSFKGLLIDLDQHTVTTNGEPMLLPPKEF
                     GVLLYCARHQGKILTKQQIYEAVWGEPYVYDDSNIMAIISRLRKKIELDPSSPIYLQT
                     IKGIGYQFNTEV"
     gene            3790..4725
                     /locus_tag="PC471_003999"
     CDS             3790..4725
                     /locus_tag="PC471_003999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF2791023.1"
                     /translation="MKMDEQTIILFLLLLSIGLLFLSIFAFSKLGHIYKRLKDIEEIL
                     ADVQIGDENRKILIKPCDVMAPLVYQLNEIVYDYENKLLSLKKSDKASKQLMTSLSHD
                     VRTPLTTLIGYLDAVHSGIVIEQEREEYLEIARRRAYDLKDYIDVLFDWFRLNSDEFT
                     LSIESVEIAELSRNILKDWIPIFHEKKLNFEIDIPENRLMVNLDPDGYSRVVNNLVQN
                     VLAHSKARQIKITMSEDSRMVLLRVEDDGVGIARENLPHIFERLYKCDKGRSEKGSGL
                     GLSIVSQMVERMGGQVSVESEIGKYTVFTVSLPLI"
     gene            4873..5796
                     /locus_tag="PC471_004000"
     CDS             4873..5796
                     /locus_tag="PC471_004000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013273760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2791024.1"
                     /translation="MNNLIIETKNLTKQYGAQKSVSNLNIHVQKGRIYGLLGRNGAGK
                     TTTMKMLLGLTKPTTGEIKIFGQDIHNNEKKLLPRIGCLIESPGFYPNLTGTENLKIF
                     ARLRGIPKKNAVESALDVVGLPNNDKKLFSQYSLGMKQRLAIALAIMHDPQLLILDEP
                     INGLDPIGIAEVRTFIRDLCTERGKTILISSHILSEIALLADDIGIIDHGILLEEESL
                     AQLEEKNSKYIHFAVSDAAQAARLLETQLNLKNFKVADNYNIMLYDTDVSVAEINRAF
                     ILNNIEVSESHLCEDTLEDYFKKVTGGEGIA"
     gene            5780..6550
                     /locus_tag="PC471_004001"
     CDS             5780..6550
                     /locus_tag="PC471_004001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2791025.1"
                     /translation="MKELLDIISCEFSKLKRLKFILISILGACLFPIPATILIAKDNL
                     PFEQLFKLVVNFGYFLLLPIVLSIVASQLFFIERDNDVLKNLATVPVQKGKLALAKLA
                     VLLFISLFYSVAGLGATIIGGFIVGIVEGVAVKLGMSIAFGIMFFVTVLPVVVLIVYF
                     NRSYIFSIILSFVYAIFNFSISLNIINFEPNNPILSVLPAPVIMRWWMAFWGDPTGEY
                     TALRQPYLLSTPACVGILCLITIIAVLLICMIYKKQEN"
     gene            6560..7342
                     /locus_tag="PC471_004002"
     CDS             6560..7342
                     /locus_tag="PC471_004002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2791026.1"
                     /translation="MLDIITTEFQKIKRYNILWIGVVAVLFSALLAVFQVVSSHGSDP
                     LTYESFANGVIWNNFSLSFPFGITLIGGYLINREYTDQTLKNILTVPISLRKLLVGKL
                     IAVGGITVLLSLFSFLCTFILGLLFCNINISVTLVVKSLVQILSVNLYCYVAVLPIIA
                     YFGRKQNAFLTGVGIAFVYGFCGVFVAGRNLTDFYPITAGLGLVGYNNGAGTVYQPLI
                     GAMTLIVILILTTILLVFTPNYDKVMTISKKKGIKHKKYSHK"
     gene            7719..8255
                     /locus_tag="PC471_004003"
     CDS             7719..8255
                     /locus_tag="PC471_004003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HDF2791027.1"
                     /translation="MNLKGTKTEKNLMAGFAGESEARNKYTYYASKARKEGYNQIAAI
                     FEETANNEKEHAKLWFKLLHDGMPSTEENLKDAAAGENYEWTDMYAKFAKEAREEGFD
                     KIAYLFEAVGKIEKEHEERYLKLLENLNEGKIFKRDEEVVWQCQNCGHVYVGTEAPEK
                     CPVCDHPKAYFNIKAENY"
     gene            8287..8676
                     /locus_tag="PC471_004004"
     CDS             8287..8676
                     /locus_tag="PC471_004004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HDF2791028.1"
                     /translation="MKFSKQRELILNEILNNPVHPTADYLYENLKKDNPNLSLGTVYR
                     NLAQLTEHGFIRKVSIPGYPDRFDGRIDNHYHIICEVCGEVYDLESEVLNNLQELISE
                     ETDIKITSYNISFKGICNNCKRCSQVG"
     gene            8713..9099
                     /locus_tag="PC471_004005"
     CDS             8713..9099
                     /locus_tag="PC471_004005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="desulfoferrodoxin"
                     /protein_id="HDF2791029.1"
                     /translation="MCSEQKFFICETCGNLVGMIQSGGVPIFCCGKPMKELVPNTTDA
                     AVEKHVPVIEVDGNNVTVKVSSTTHPMTKEHHIAWVYLMTEQGGQRKCLAVDGEPVVK
                     FALNDDDKVISAYAYCNLHGLWKAEL"
     gene            9226..10668
                     /locus_tag="PC471_004006"
     CDS             9226..10668
                     /locus_tag="PC471_004006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HDF2791030.1"
                     /translation="MSIYKCSVCGYIYDESKNDKTWDELSEDWECPVCTKGRSYFGKI
                     STVYYEEDEKIAEDIVEDESKLNTEKEGDLNYLSTYLRRDDEVEKHMDIIHEMAVTGK
                     SIIEPMRTKLPVISWDDILIMGAQLNPLPLNEHDEVNTTTIIGKKAKKPMIIENPVYI
                     SHMSFGALSKELKIALAKGAAQNKTAMCSGEGGILPEEKEASYKYIFEYVPNKYSVTE
                     ENLKNSDAIEIKIGQGTKPGMGGHLPGEKVTEEIAKVRNKPVGQDVISPSCFEEIQSK
                     EDLKKLVDELREVSEGRPIGVKISAGHIEKDMEFIAYAKPDFVTIDGRGGATGASPKL
                     LKDATSIPTIFALYRARKYIDTHGLDIDLVITGGLRISTDFAKAIAMGADAVAIASSA
                     LMAAACQQYRICGSGKCPVGVATQDEELRKRLHIENSANRVANFLNVSLEELKTFARI
                     SGHKDIHDLSVDDLYTVNSEISNYTNIQHV"
     gene            10965..11780
                     /locus_tag="PC471_004007"
     CDS             10965..11780
                     /locus_tag="PC471_004007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDF2791031.1"
                     /translation="MERLKIQVLVDNNTYIDRYFVGEPAVSYYIEIDGNRILFDSGYS
                     DVFISNAEKLNIDLGNLTHVVFSHGHNDHTRGIQFLENRYDLSTVELISHPNCFIPRK
                     HGEKSIGAPFSAEEIKNIFMYNPKDKPFNLSKNCVFLGEIPSINDFEKRAKIGKCKIG
                     DLWEDDYVLDDSAIVCKTDKGIFIVTGCSHSGICNIVEYAKKVCGDDRVIGILGGFHL
                     FELNNRLDSTIQYLDKEEMRMLYPCHCVSFKVKAKMNEILNINEVGVGLTISI"
BASE COUNT         4355 a         1494 c         2279 g         3958 t
ORIGIN      
        1 aatatagagt aagtttattg taatgaattg ttaaagaaaa taattaatat agggtaaatc
       61 tatctcaatg aaataacaaa gaaaagagtt aatatagagt gaacttattg caatgaatca
      121 acaaagaaaa taatcaatat aaaaaggaga aatatttatg aatacaggat ttccaaaaga
      181 ctttttatgg ggagcatcat cttctgcatt tcaagtggaa ggagcttggg ataaagacaa
      241 taaaggaaaa accgtagccg actataattc ttttaaaaag tctcatttac aggcagatac
      301 taaagtcgca tcagactttt accataatta tgaagaagat attgaattaa tgaaagaatt
      361 agggatgaaa acatatcgtt tttcaatttc atgggcaaga ataataccag atggtgaagg
      421 agagataaac cagaaaggtt tagaatttta taataagata atagacaaac tgatagagtg
      481 tgatattgag ccattcgtaa ctttatacca ttttgacctt ccatttaaat tagtagagaa
      541 atataatggt tgggaaagta gagaaactgt ctatgctttt gaaagatttg ctaaaatatg
      601 ctttaagcat tttggagata gggtaaagta ttggcaacct cataatgaac agaatttaat
      661 tgttagagtg gaggagagaa ttaatatata tgatgaaaca gattcatgga agatagataa
      721 aatgcgtgct cagatggatt ataatctttg cttagcacat gcattagcag tcaatgcatg
      781 tcatgagatg ataaaagaaa gtaaaattgg agcagcagta tcatcatctg taacatatcc
      841 tcttacttct aagccagaag atgtatatgc agctagaatg aatgataatt ttaaagtgta
      901 ctatatgctt gatatgcatc attatggaga atatccaggg tattatatga agtatcttga
      961 aaaaagaaat atagttcctc acatggaaga tggggataaa gagatactta aaaaagctaa
     1021 aatggatttt atagctgtaa attattatag gacaaattgt gcagaagctt tacctgaaga
     1081 ttcacaacat ccatttggat taagagaagg aactgttgat tttagtatgt atggactatt
     1141 taaaatgtca atgaatccta acttagaggc ttctgagtat ggagcagcaa tagacccatc
     1201 aggtcttaga gttgccttaa atgaatattg gcaaagatat cacttacctg ttataataac
     1261 agaaaatggt ctaggagcta aagatatatt agaagatgga aaaattcatg atgactatcg
     1321 tatagactat ttaagaagtc atataaatgc atgtaaacta gctattgaag atggtgtaga
     1381 gatgattgga tattgtccat ggtcatttac agacctttta agttcttctc aaggttttaa
     1441 taagcgttat ggacttgttt atataaatag aactgaccat gaagttttag atttaaaaag
     1501 aatcaagaaa gatagttttt actggtataa agaagttata gagaacaatg gaatagtaaa
     1561 ataaacataa atatatattg agggttttag tatgtacatt acaaaggtat taaataattc
     1621 gttgctactt gcaaaggatg ataatgatga agaaataata ttgatgggga aaggcattgg
     1681 ccataattat aaggccgggt ctgagttaaa taaaaaagat atagaaaaga tttttgttct
     1741 tcatgatgaa aatataaaaa agagtattat acaattggca acagaaatag atgcagaata
     1801 ttttggtata gctcaaatga taattgcata tggaatagaa aaatataact taaaacttat
     1861 gaaccatata tatttggcct taactgacca cattgccttt gcagtgagac gttttaaaga
     1921 aggtataatg attgaaaatc actatttgtt cgaaatcaag gaatttaatc caaaagagta
     1981 tgatatagga aaatatgcta ttactgtttt taaggaggtt cttggtttag acctaccaga
     2041 agaagagatt ggtaatatag caggccattt tataaatgca caacaagata atccttatag
     2101 tgatagaaat aagagaagtg ctaaaattgt aaatgctatt ttgcaaatag ttcattatca
     2161 cttttctatt gtatataata aagagtcatt ttattatagg agatttgtaa tgcatctaaa
     2221 ggccttttca caaagatttc tggctaatga accatcaaga gaaaaaatag attttatata
     2281 tgagcaagtt caaaaaaatt gtaaggaaga gtatgaatgt gtgaaaaagg taggagctta
     2341 tatgtataag gaatactcca gagaattacc aagacaagag gagttatatt tgatgattca
     2401 tattcataag attttaggag aattatgcga aaatagttag aatatcttaa tatgctagta
     2461 atattataaa aaattaaaaa ttaactgcac tttaaatatg aataattgca ttacttaagg
     2521 gggttgtctt aaaattgatt tttttaatta aatttgaagc tcaactcctt ttttaaacct
     2581 ttatatcatt acaactttaa tgtttatacg actgtacttt agatgtatat ctacttttta
     2641 catttctcaa catgcaaacc taccattttt tgcagtatgt taattgaaat aaaccattga
     2701 gttaacccta aatttattct ggacttatag gaatagttcc aaagatactt taaaatattt
     2761 tctggaataa ttgcttattt cacatatgtt ttattgtgat gttttgattt aataaattct
     2821 tcaactctgt atatagtaaa tgtattatca tgtttgtttt attcaagcac acttataata
     2881 cgcttgattt tttcacaatg atgaaatata ctaatgggtg ttataggtat aagaatcgca
     2941 ttacacttga agaagtaagt gataaatgag gtataaatcc tcatatgata aactactatt
     3001 tcattgagga acatattcta agtgatgtca agatactaca atatgactaa attcctagtg
     3061 ataaaaaagt ctacgaatga gtgtagaaag gggaactaag atgaataaag ttttaattat
     3121 agatgatgat aaagatcttt gtattttgtt aaaaaagagt atttcaattg aacagattga
     3181 agcaaactat tgccattctg ggagagatgg actggttgaa ctttcaaaac aagattatca
     3241 gttagtagtt ctagatgtga tgatgccagg tatggatggt tttgaaacta tggaacaaat
     3301 acgaaaatat agcagtattc ccatcttaat gctgacatca aaaagcgata attacagcaa
     3361 agtgcatgga ttgcgagctg gtgcagatga ctatctaacc aaaccatttg aaattacaga
     3421 atttactgct cgtgtactgt ctttaatccg acgttacacc cgttttaatc caaaaaatga
     3481 ggagccacag caactttctt tcaaaggttt gttgattgac cttgaccagc atacagttac
     3541 taccaatggc gaacctatgt tgcttccacc caaggaattt ggtgtacttc tttactgtgc
     3601 aaggcatcag ggtaagattt tgacaaaaca gcagatttat gaagccgtgt ggggtgagcc
     3661 atatgtatat gatgacagca atatcatggc tattatcagt cgcctaagaa aaaaaattga
     3721 actagaccca tcatctccca tctatttgca aacaataaaa ggaattgggt atcaattcaa
     3781 cacggaggta tgaaaatgga tgaacaaacg ataattcttt tcttactctt gctgtcgatt
     3841 ggtctacttt ttctttccat atttgctttt tccaagcttg ggcatattta caagcgccta
     3901 aaagatatag aggagatact ggctgatgta caaattggag acgaaaatcg taaaatacta
     3961 attaagccat gtgatgttat ggctccattg gtttatcagc ttaatgagat tgtctacgat
     4021 tatgaaaata agttactttc tctgaaaaag tcggataaag ctagcaaaca acttatgaca
     4081 agtttatctc atgatgttcg tacaccatta acaactctga ttggttatct ggatgctgta
     4141 cacagtggta tagttatcga acaagagcgg gaggaatacc ttgaaatcgc taggcgtaga
     4201 gcctacgacc tcaaggatta cattgatgtt ctttttgact ggtttcggtt gaactctgat
     4261 gaatttacgc tgtcaataga atctgtggaa attgcagagt tgtcacgcaa tattttgaaa
     4321 gactggattc ctatattcca tgaaaagaaa ctgaattttg aaattgatat tccagaaaat
     4381 cgactaatgg taaacctcga cccagatgga tattcccgtg tggtcaataa tctggttcaa
     4441 aatgttcttg ctcacagtaa ggctcggcaa atcaagatta ccatgagtga ggattccaga
     4501 atggtattac tccgtgttga ggatgatggt gttgggattg ccagagaaaa tttaccacat
     4561 atttttgagc gactgtataa gtgtgataaa ggacgttcag aaaaaggaag tggtcttggt
     4621 ctatctattg tcagtcaaat ggtggagcga atgggtggtc aggtttccgt agaaagtgag
     4681 attggaaagt ataccgtatt tacagtgagc ttaccattaa tataaaattg tttttccccc
     4741 atgtgacatg tatacacatg gggatttttt gtaagttata aagttgcaag gttaatacaa
     4801 ggttgttgca aggttaatgt aagattgagg tggtatgata actttaataa acaaaggagg
     4861 cttttattat ttatgaataa tttaattatt gaaacaaaaa atctcactaa gcaatatggt
     4921 gctcaaaaga gcgtttctaa tttgaatatt cacgttcaga aaggtcgcat ttacggattg
     4981 ctaggaagaa acggtgcggg taagacgaca actatgaaaa tgttattggg tctaacaaaa
     5041 ccaactactg gagaaattaa aatatttggt caggacattc acaataacga gaagaagctt
     5101 cttcctcgaa ttggatgttt gattgaatca ccagggtttt atcctaatct gacaggaaca
     5161 gaaaatctca aaatctttgc aaggcttaga ggaataccaa aaaaaaatgc agtagaatca
     5221 gcactggatg tagtcggttt gccaaacaat gacaaaaagt tattttctca atactctctt
     5281 ggtatgaaac agaggttggc gattgctctt gccattatgc atgacccaca actgttgatt
     5341 ttggacgagc ctatcaatgg acttgatcct attggtattg cagaagtaag aacgtttatt
     5401 cgtgatctct gcacagaacg aggtaaaact attttgattt ccagtcacat tctttctgaa
     5461 attgccttgt tggcagatga cattggcatt attgaccatg gtattttgtt ggaggaagaa
     5521 agtcttgccc aattagaaga aaaaaacagt aaatacatac attttgctgt atctgatgca
     5581 gcacaggcag caaggcttct ggaaacccaa cttaacctga aaaatttcaa agtagcagat
     5641 aattacaata ttatgctata tgatacagat gtttctgttg cagaaataaa tcgtgctttc
     5701 attctaaaca acattgaggt atcagaatct catttatgcg aagatactct tgaagattac
     5761 ttcaagaaag taacaggggg tgaaggaatt gcttgatata atttcatgtg aattttcaaa
     5821 actcaagcgg ttaaaattta tactgatttc tattttaggt gcttgcttat ttccaattcc
     5881 cgcaacaatt ttgattgcaa aggacaactt gccctttgag caattgttca aattagttgt
     5941 caattttgga tattttttgc tattaccaat tgtattaagt attgttgcta gccaactatt
     6001 ttttatagaa agagataatg atgtattaaa aaatcttgca acagtgccag tgcaaaaagg
     6061 aaaactggct ctggcaaaat tggctgtgtt gctgtttata tcattgttct actctgttgc
     6121 agggttagga gctactatca ttggaggttt cattgttgga atagtagagg gggttgctgt
     6181 aaaattagga atgagcattg cttttggaat tatgtttttt gttactgtac ttcctgttgt
     6241 ggtactcatt gtatatttca atcgcagcta tattttttct attatacttt cttttgtata
     6301 tgctattttc aattttagta tatccttaaa tattatcaat tttgaaccaa ataacccaat
     6361 attaagtgta ttacctgctc ctgttataat gcgttggtgg atggcatttt ggggagatcc
     6421 cactggcgaa tacacggcac ttagacagcc atatttgctc tcaactcctg cttgtgtagg
     6481 tattttatgt ttgataacca ttatagcagt cctgctgatt tgtatgatat acaaaaagca
     6541 ggagaattag gaggtatcta tgcttgatat aattactaca gaatttcaaa aaataaaaag
     6601 atataatatt ctatggattg gtgttgttgc tgttctgttt tcagcactat tggcagtttt
     6661 tcaggttgta agttcacacg gcagtgaccc tctaacgtat gagagctttg caaacggagt
     6721 tatctggaat aatttcagtc ttagttttcc atttgggatt acacttattg gaggatatct
     6781 tattaaccgt gaatatacag accaaacctt aaaaaacatc ctaactgttc ctatttcatt
     6841 acgcaaattg ctagtgggaa aattaattgc ggttggagga attactgtat tgttaagtct
     6901 tttcagcttc ttatgcacat ttattttggg gttgttgttt tgcaatatca atatatctgt
     6961 tacactcgtt gtaaaatcct tagttcagat tttgtctgtt aatctgtatt gttatgttgc
     7021 tgttttgccg attattgctt attttggtcg taaacaaaat gcatttctga cgggtgtagg
     7081 gattgcattt gtatacggtt tttgtggtgt gtttgttgct ggaagaaatc tgacagactt
     7141 ttatccaatc acagcaggtc ttggtctagt tggatacaac aatggtgctg gtactgttta
     7201 tcagccactt atcggtgcta tgactttgat tgtaattcta atcttgacaa ctattctact
     7261 tgtatttaca cctaattatg ataaagttat gaccatatct aagaaaaaag ggataaagca
     7321 taaaaagtat tctcataaat aacataaata ttgtttctat tatttaagtt gctagacagt
     7381 gaaaagaaaa aatctgtcgt acagtgtcta tatgctcatg tattcaagaa gctacagttt
     7441 tgatttttca taactgtagc ttctttttgt atttattaaa tcacgactat cataatactt
     7501 gcaataggta tagcgacaag agagagtact gtcatatgtg ttttatatat tacattaagt
     7561 atattctaaa atatatgttt taaataatat atttgaaaat aggaatattt aaataaaaaa
     7621 aataatatta tatatagttg acaaaaattg gcaaatgaga taatatataa atatagaaat
     7681 agtaatcatt actattatta aaaagggagg aattaattat gaacttaaaa ggaactaaaa
     7741 cagaaaagaa tttaatggca ggatttgcag gagagtcaga agcaagaaat aaatatacat
     7801 actacgcttc aaaagcaaga aaagaaggtt ataatcaaat agctgctata tttgaagaaa
     7861 ctgctaacaa tgaaaaggaa catgctaaat tatggtttaa acttttacat gatggtatgc
     7921 cttcaacaga ggaaaattta aaggatgcag cagcaggtga aaactatgaa tggactgata
     7981 tgtatgcaaa atttgctaaa gaagctagag aagaaggatt tgacaagata gcttatttat
     8041 ttgaagctgt tggaaaaata gagaaagagc atgaagaaag atacttaaag cttttagaaa
     8101 acttaaatga aggaaaaata tttaaaagag atgaagaagt tgtttggcaa tgtcaaaact
     8161 gtggtcatgt gtatgtaggt actgaagcac cagaaaaatg cccagtatgt gaccatccaa
     8221 aagcgtattt caatataaaa gctgaaaatt attaaaaatt atcattatta aaaaggaatg
     8281 ttaactatga aattttctaa acaacgagaa ctgattttaa atgaaatatt aaataatccg
     8341 gttcatccta ctgcggatta cctatatgaa aacttaaaaa aagataatcc aaatttaagc
     8401 ttaggaactg tgtatagaaa tttagctcag ctaacggaac atggctttat aagaaaggtt
     8461 agtattccag ggtatccaga tagatttgat ggtcgaatag ataatcacta tcatatcata
     8521 tgtgaagtat gtggagaggt atacgattta gaatccgaag ttcttaataa tttacaagaa
     8581 ttaatatctg aggagacaga cataaaaata acatcttata acataagttt taaagggatt
     8641 tgtaataatt gtaaaaggtg tagccaagta ggttagtttt caaattctaa aaataattaa
     8701 tgaggtgagt ttatgtgtag tgaacaaaaa ttttttatat gtgagacttg tggaaacctt
     8761 gtaggaatga tacaaagtgg tggtgtacct attttctgtt gtggaaaacc tatgaaggaa
     8821 cttgttccta atacaacaga tgcagcagtt gaaaaacatg tacctgtaat tgaagtggat
     8881 ggaaataatg ttactgttaa agtaagtagc acaactcatc caatgactaa agaacatcat
     8941 atagcatggg tgtatcttat gactgaacaa ggtggtcaac gcaaatgtct agcagtagat
     9001 ggagaacctg tagttaaatt tgctttgaat gatgatgata aagtgatttc tgcctatgct
     9061 tactgtaatt tacatggatt atggaaggct gaactgtaat atttaaataa attgagtcaa
     9121 aaaggaggct ataaatgtat tttcatacaa atagcctcct ataaatttat ataaattcaa
     9181 gttttaattt tgtttaatgt caatataata taaggagaaa ataatatgtc aatttataaa
     9241 tgtagtgttt gtggatatat ttatgatgaa tctaagaatg ataaaacatg ggatgaatta
     9301 agtgaagatt gggaatgccc tgtatgtaca aaaggtcgta gttattttgg aaagataagt
     9361 actgtttact atgaagaaga tgaaaagata gcagaagaca tagttgaaga tgaatctaaa
     9421 ttaaatactg aaaaagaggg agatttaaat tacttaagta catatttaag aagagatgat
     9481 gaagttgaga aacatatgga tataattcac gaaatggctg ttacaggaaa atctataata
     9541 gaacctatga gaactaaact accagtaatt tcttgggatg atattttaat tatgggtgct
     9601 caattaaatc cattaccttt aaatgagcat gatgaagtaa atacaacaac aataatagga
     9661 aaaaaagcaa agaaacctat gattatagaa aatccagttt atatttccca catgtcattt
     9721 ggagctttgt ctaaagaact aaaaatagct ttagcaaagg gtgcagccca aaataagaca
     9781 gctatgtgta gtggtgaagg aggtatatta ccagaagaga aggaagcttc atacaagtat
     9841 atttttgagt atgtaccaaa taaatatagt gtaactgaag aaaatcttaa aaattctgat
     9901 gctatagaga taaaaattgg tcaaggaaca aaacctggta tgggaggtca tcttcctggc
     9961 gaaaaagtaa ctgaagaaat agcaaaagta agaaataagc ctgtaggtca agatgtaata
    10021 agtccatctt gttttgagga aatacaatcc aaagaagatt taaagaaact tgtagatgaa
    10081 cttagagaag tttcagaagg gcgtccaata ggtgttaaaa tttcagcagg tcatattgaa
    10141 aaagatatgg aatttatagc ttatgctaaa cctgattttg taactattga tggtcgtggt
    10201 ggagctactg gagcaagtcc aaagttgctt aaagatgcaa cttcaattcc aacaatattt
    10261 gctctttatc gtgctagaaa atacatagat acacatggat tagatataga tttggttata
    10321 acaggtgggc ttcgtatatc tactgatttt gctaaggcaa ttgctatggg tgctgatgca
    10381 gttgctattg caagttctgc tcttatggca gcggcgtgtc aacaatatcg tatatgtggt
    10441 tctggtaagt gtcctgtagg agtagctact caggatgagg aattgagaaa aagattacat
    10501 atagaaaaca gtgctaatag agtggcaaac tttttaaatg taagtttaga agaacttaag
    10561 acttttgcta gaatttctgg tcataaagat atacatgatt taagtgttga tgatttatat
    10621 actgttaact ctgaaatatc aaattataca aacatacagc atgtatagat tgaagtttga
    10681 aactattaga ttttagatag ctataaaagt aaatgataaa tatattttat aaaagagagt
    10741 gtcttaaaat gaacttttta gttcataagt cactcttttt tttatgaagc caaatatatt
    10801 tatcatttta gcaatgaaaa agaatcttat ctctttattt tgagatagac tccttctatt
    10861 tatgaaaatt aaagatatta aattgtaaaa tattctgtga tgtcgtacta ttaaactaaa
    10921 gattctgtac tttaaagtaa ataaaaattt aaggagtgat tattatggaa agattgaaga
    10981 tacaagtttt agttgacaat aatacttata ttgatagata ctttgttgga gaaccagcag
    11041 taagctatta tattgagatt gatggaaata ggattttgtt tgactcaggg tattcagatg
    11101 tattcatatc aaacgctgaa aaattaaata tagatttagg aaatttaact catgttgttt
    11161 tttctcatgg gcataatgac catacaagag gtatccagtt tctagaaaat aggtatgatt
    11221 tatctacagt tgaacttatt tcacatccta actgttttat acctagaaaa catggagaga
    11281 aaagcatagg agctccattt tctgctgagg aaattaaaaa tatttttatg tataatccta
    11341 aggataagcc atttaatcta agtaagaatt gtgtgttttt aggtgaaata ccttctataa
    11401 atgattttga aaaaagagct aaaattggta aatgtaaaat aggagatttg tgggaagatg
    11461 attatgtttt agatgattct gctatagttt gtaaaactga taaaggtatt tttattgtta
    11521 caggatgttc tcatagtgga atttgtaata tagtagagta tgctaaaaag gtatgtggag
    11581 atgatagagt tattggtata ctaggtggtt ttcatctttt tgaattgaat aataggttag
    11641 atagtacaat acaatatttg gataaagaag aaatgagaat gttgtaccct tgtcactgtg
    11701 tttcttttaa ggtaaaagcg aagatgaatg aaatattgaa tataaatgaa gttggagtag
    11761 ggctgaccat aagtatatag tatttgaaag tatataagat ttaagttgaa ggttaaaaag
    11821 atattttcta tagaaaatat cttttttgtt acgtttattt taagagaaat caatatactt
    11881 ttatatgaaa tattgtaaaa aaatttattt ttaatgagaa tatattttca ttaataaaca
    11941 attttattat gtattaataa tgttagaata aatataaata aatcaaagtt acttacatta
    12001 cagtatattt tttcacaaaa tatattgatt tattctttta aagtttgata attccaatga
    12061 atgtaataaa agaaggaatt ttatac
//
LOCUS       DAMJYR010000335        11087 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933282 isolate JH368
            SAMN32366184-rid16591183.guided.123, whole genome shotgun sequence.
ACCESSION   DAMJYR010000335 DAMJYR010000000
VERSION     DAMJYR010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366184
            Sequence Read Archive: SRR22862940
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11087)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11087)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560796.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560796.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:31:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,892
            CDSs (with protein)               :: 3,892
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 15 of 45
            Pseudo Genes (incomplete)         :: 20 of 45
            Pseudo Genes (internal stop)      :: 19 of 45
            Pseudo Genes (multiple problems)  :: 8 of 45
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11087
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366184-rid16591183.guided.123"
                     /strain="L,13.7933282"
                     /isolate="JH368"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            216..554
                     /locus_tag="PC471_004008"
     CDS             216..554
                     /locus_tag="PC471_004008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2791032.1"
                     /translation="MANIGKIIGDRIKVYRTKLGYSQEFLAEKAGLHPTYIGQIERAE
                     TNTSINIIMKIATALDIPLELLFANIITTEGVDNSVPLECYEMINKLTEKEQIAMLEL
                     IKCIIKYKEI"
     gene            576..893
                     /locus_tag="PC471_004009"
     CDS             576..893
                     /locus_tag="PC471_004009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2791033.1"
                     /translation="MNYIGKRLKEERKKANLTSKEFANMVGVSPWYITQIESGKKNPS
                     LKTFIKFVNILNISADVLIKDITSTGKTYLENDINEELKDLNSRELNLISQIIDIIKK
                     NKI"
     gene            1043..1720
                     /locus_tag="PC471_004010"
     CDS             1043..1720
                     /locus_tag="PC471_004010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2791034.1"
                     /translation="MIVIEGSDKFKIAKEYIDVEYTLFSKVTFRYEKLKFKDNAELEK
                     IKMFKYKNGYIPNKLNLSFGYGFSSYKKQIIRETVDTLRLTEIFSSENIEDIKFIKDG
                     TKKLEISIEKVVKFKRRKKRNYVCCYCPDMYRDIKLDKESINKIYNRKIKIEREVNIF
                     EDEDVIINKKVLKFPKSWTKNMQKYWLSENKYPIHSTVIDDDRYKCCNVQYTKNRVII
                     LYYIYNH"
     gene            complement(2048..2815)
                     /locus_tag="PC471_004011"
     CDS             complement(2048..2815)
                     /locus_tag="PC471_004011"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HDF2791035.1"
                     /translation="MKRKVQVKNITIGEGRPKICVPIIGKNKKDIIKEAKELKDACLD
                     IIEWRVDFFENVENIKEVKEVLYELRSYIHDIPLLFTFRSVVEGGEKLISRDYYTTLN
                     KEISNTGLVDLIDVELFMGDEVIDEVVNFAHKKEVKVIISNHDFNKTPKKEEIVSRLC
                     RMQELGADLPKIAVMPQNEKDVLVLLEATNEMFKIYADRPIITMSMSGMGVISRLCGE
                     IFGSALTFGAAKSVSAPGQISFKELNSVLNLLHKSIN"
     gene            complement(3487..4302)
                     /locus_tag="PC471_004012"
                     /pseudo
     CDS             complement(3487..4302)
                     /locus_tag="PC471_004012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897377.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
     gene            4403..5101
                     /locus_tag="PC471_004013"
     CDS             4403..5101
                     /locus_tag="PC471_004013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDF2791036.1"
                     /translation="MYKLSYVVRVSTKNLDELKRRCDEVKDFYDDLSIKLVRPFGDML
                     GLHGEFIPVSKRYINDYIQYVTSDFLAGLGFGATQTLGEHEDIYLGYNLDTGKNVYLK
                     PALASQGVKGSITNALASAFIGSLGGGKLFSNNMLVYYAVLFGGQAVIVDPKAERGKW
                     KETLPEIAHEINIVNLTSNDENKGLLDPYVILSRKKDSESLAIDILTFLTGISSRDSD
                     KFLQKNDNWKIIME"
     gene            5189..5809
                     /locus_tag="PC471_004014"
     CDS             5189..5809
                     /locus_tag="PC471_004014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HDF2791037.1"
                     /translation="MHLIDFMNEDIKKKLVKKTYKKKETILFAEQENEYVILMIDGIA
                     EAFILNVKGNISTIHLYRSGSFFGEMEQFNDGKKPVEIVAFTDCNVYKLHRDDFLNWL
                     RGDFEATKFLIREISSKLVVNAELIEELSSLSVKERLLRCISLHYYRSELNLLTKKQL
                     ASEVNAPIRSINRAINECVQEHLIAYENKRFSVINQREVLKNLPTT"
     gene            5965..6747
                     /locus_tag="PC471_004015"
     CDS             5965..6747
                     /locus_tag="PC471_004015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside 6-adenylyltransferase"
                     /protein_id="HDF2791038.1"
                     /translation="MTKTQQKMIQNVLEFSKRDSRIIGLAISSSYITKSLDEYSDLDF
                     LIVADDKSYEEVIEERLLIVEQFGTLVSAFTGEHVGEPRLIISLYDSPVLHVDFKFTT
                     LDGLLDRVEDSAILYEENNCITEIYSKKEAYFPTPDLQWIEDRFWIWVHYACTKIGRR
                     ELFETIDFLSFLRQSVLAPLIQLLLGKLPRGVRKIELDAPQYIEALEKTVANHEYKSC
                     VNALQATINLYLELREELSQSPIVRKVEAERVALQYFQDLIK"
     gene            6872..7798
                     /locus_tag="PC471_004016"
     CDS             6872..7798
                     /locus_tag="PC471_004016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2791039.1"
                     /translation="MSDILSRITNKIGDKNIVDKLSALSKSDLNSLLLEVFDRQANTL
                     TATDILKSYQLNRFTIPSGIDPEEIHALESKLLRKAFNMDIKTIMLSPSAPLGSCSVF
                     GSVDQYNVVSALRGTETLADPSNMLAIIIADKLKRKEENNTIPIHMATTARVIRAQNF
                     VGKGLYSHFGLYCMVSSGIDTGSYTCEKMLLEKHLNYYKDILSEIENVRFSIILRKRN
                     GYKDNDGFFDRMVETIKLIFPNIELSIHNDDVDNNYYKGINFKLSVKQGNETVEIVDG
                     GFVDWTYQMLGNKRERCLISGIGLERFLVAVS"
     gene            7812..8261
                     /locus_tag="PC471_004017"
                     /pseudo
     CDS             7812..8261
                     /locus_tag="PC471_004017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729090.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            8621..9028
                     /locus_tag="PC471_004018"
     CDS             8621..9028
                     /locus_tag="PC471_004018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HDF2791040.1"
                     /translation="MVDEKTIEHQFDSFCKTVLRNYARDIYDENKRRNDYLVSLESLS
                     LAELSKLSILDDYDSNYICMVSYDYNIRIEDVLMAQAIGKLTKRKQDIILLSFFFNMT
                     NADIATLMDLAENTIHYHKTNALKELKELMQEH"
     gene            9030..9260
                     /locus_tag="PC471_004019"
     CDS             9030..9260
                     /locus_tag="PC471_004019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF2791041.1"
                     /translation="MKPTKEVLPFTIILLAVHGDVNAINVILKHFEHYIIRLSQKTLF
                     DEYGNPYIHVEEETKRMLGTKLITAILRFNLS"
     gene            9650..9853
                     /locus_tag="PC471_004020"
     CDS             9650..9853
                     /locus_tag="PC471_004020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HDF2791042.1"
                     /translation="MNNTDVPIWEKYTLTIEEASKYFRIGEKKLRKRADENLDSGWVI
                     MNSNRIQIKRKQFEKIVDTLDVI"
     gene            9931..10284
                     /locus_tag="PC471_004021"
     CDS             9931..10284
                     /locus_tag="PC471_004021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase DNA-binding domain-containing protein"
                     /protein_id="HDF2791043.1"
                     /translation="MSTEKRRDNKDRILRTGESQRKDGRYAYKYVYSFGKPQFVYSWK
                     LVPTDKPPKGKRDDISLRDKEKAIQNDLDDSIDTIGKKMTVCQLYAKKNGLRKNIRLN
                     TKKGRHYLMKILNKE"
     gene            10356..10988
                     /locus_tag="PC471_004022"
     CDS             10356..10988
                     /locus_tag="PC471_004022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CatB-related O-acetyltransferase"
                     /protein_id="HDF2791044.1"
                     /translation="MTIPSSNKVYPRSNDYQTVYLKNVITRENIEVGDYTIYNDFYND
                     PRDFEKNNVLYHYPINNDKLIIGKFCSIACNAKFLMTSGNHSMKSKSTYTFPIFYEEW
                     KLDINHITDAWDNKGNIIIGNDVWIGYDSIIMSGVNIGDGAIIGTRAVVTNDVPPYSI
                     VGGVPAKIIKKRFNNDTISKLLEIKWWDWPYKKIQSNIKHIQSGNIDKLV"
BASE COUNT         4083 a         1570 c         1744 g         3690 t
ORIGIN      
        1 ttattgtagt atggctatga aattatgaaa atatttctct aattaaaaat atgatttatc
       61 catatttgtt tattgtagat acagactata caaaaataaa tagcacttat ttcagtataa
      121 aaacatatat agtctatatt tagactaaaa aagtataaat agtctatata aaacaactta
      181 aactatgaat ataatctaaa gtggagggat aataattggc aaatatagga aaaataatag
      241 gagatagaat aaaagtctat agaacaaaat tgggatatag tcaagagttt ctagcagaaa
      301 aggcaggttt acatccaaca tatattggtc agattgaaag agctgagaca aatacatcta
      361 tcaatattat aatgaaaatt gctactgctt tagacatacc acttgaattg ttatttgcaa
      421 atattattac aacagaaggc gttgataact ctgtaccttt agaatgttat gaaatgatta
      481 ataaattaac tgaaaaagaa caaattgcaa tgctagagtt aataaagtgc atcattaaat
      541 acaaagaaat ataagtgagg gaaatagtaa caataatgaa ttatataggt aaaagactta
      601 aagaagaacg gaaaaaagca aatttaactt caaaagaatt tgcaaatatg gttggagtat
      661 cgccttggta tataactcaa atcgaaagtg gtaaaaagaa tccaagtctt aaaacattca
      721 taaaatttgt aaatatatta aatatttctg cagatgttct tataaaagat attacttcca
      781 ctggaaaaac atatttagaa aatgatataa atgaagagtt aaaagactta aattcaagag
      841 aactaaatct gatatcacag attatagaca tcataaaaaa gaataaaata taattcttta
      901 tattttatat aaaaaaaaag gttatttgaa ataaataacc tttttttgtc tttttgtgtt
      961 aacattgtct tatagtgcta atgagtacta ttagaccatg ttctatggcc ttattttaat
     1021 ataaaacgat tggagatagg ctatgattgt gattgaaggt agcgataaat ttaagattgc
     1081 aaaagaatat attgatgtag aatatactct ttttagcaaa gtaactttta ggtatgaaaa
     1141 gttgaaattt aaagataatg ctgaattgga aaaaattaag atgtttaaat ataaaaatgg
     1201 ctacatccct aataaattaa acctttcttt tggatatgga ttctcttctt ataaaaagca
     1261 aataattaga gaaactgtag atactttaag attgacagaa attttttcaa gcgagaacat
     1321 agaagatatt aaatttataa aagatggtac aaaaaaatta gaaattagca tagagaaagt
     1381 tgtgaaattt aaacgtcgaa aaaaaagaaa ttatgtttgt tgctattgcc ctgatatgta
     1441 tagagacata aaactcgaca aagaatctat caataagata tacaacagaa aaataaaaat
     1501 agaaagagaa gttaatattt ttgaagatga ggatgttata ataaacaaga aagtattgaa
     1561 gtttccaaag tcttggacaa agaatatgca aaaatattgg ttaagtgaaa ataagtatcc
     1621 catacattct actgtaattg atgatgatag atataaatgt tgtaatgtac aatatacaaa
     1681 aaatagagtg ataatattat attacatata taaccattaa tatacataat tacaaaaaaa
     1741 taatgacaaa tatacataat acaaatatat cttctacttt agataaaaca gttattatga
     1801 aaacttacta tctataaata atagaaatgc tagtatacca tctataatct aatgtctagt
     1861 aggttttaat ggcatatttt aaatatgcca tagttgattt ataagatttt ctatatcact
     1921 ttcaaaatac acactaaata gttgcttttc agatataatt gcccaattta aattttacta
     1981 tctgaaaagt tttttatatt taatttatat cttcaaaaat accgtctaat atttctatat
     2041 aattaattta attaatactc ttatgtaata gattaagtac tgaatttaac tctttaaatg
     2101 atatttgccc tggtgctgaa acactttttg cagctccaaa agtcaatgca gaaccaaata
     2161 tttcaccaca caatctactt ataacaccca ttcctgacat tgacattgtt attattggtc
     2221 tatctgcata aattttaaac atttcatttg tagcttcaag tagcactaac acatcttttt
     2281 cattttgcgg cataacagct attttgggta aatctgcgcc caattcctgc attctacata
     2341 aacgagatac aatctcttct ttttttggag ttttattaaa atcatgatta gatataatta
     2401 ctttcacttc ttttttatga gcaaaattaa ctacttcatc tatgacctca tctcccataa
     2461 ataactctac atctattaaa tctactaaac ctgtattaga aatttcttta ttcaaagtag
     2521 tataataatc tcttgatatt aatttttctc ccccttctac tacacttcta aatgtgaata
     2581 ataaagggat atcatgtata taacttctta attcatagag aacttcctta acttctttaa
     2641 tattttctac attctcaaaa aaatcaactc gccactctat aatatccaaa catgcatctt
     2701 ttaactcttt agcctctttt ataatatctt ttttattttt cccaataatt ggtacacaaa
     2761 ttttaggtct accttctcca atagtaatat tttttacttg aacttttctt ttcatattct
     2821 cctccataat cagaaattct ataggacttt aaattctatt ttctgcacaa tttaattata
     2881 cattaaaaat actatacatt ataaaaatac taatttaaaa ataattttta ttctaattct
     2941 taaaacaatc ccacatattt tagaaaaggt actctataat atacaaacac ttctttttta
     3001 cctaaattta ttttaacatc ctataatttt cacaatattc tttaaatttt actcatataa
     3061 acaatattat tctggatgct aatagaatta atctataaat catagtataa ttatgcagaa
     3121 ataactcgaa tttcactact tatgaagaga ttgaatatta atgaatctaa atcatttgca
     3181 ttacttaaga gttttgacta aagttgaaca ccatacaaaa gtagctactc aattgtttat
     3241 accccaacca agcttaagta atgttatata gattctcgaa aaagaatttg gtatcttttt
     3301 atttgaaaaa cttatattaa atttgattat cttttcataa gttggtaaat gtcgatatat
     3361 cctgtatcta caaggagtat cggcattttc tttttggaag atactttgca taagttggca
     3421 tttaaccgta taagaatgcc atcaaaagta gtaaataagt agtaaactct agtcatacta
     3481 ctaggattat aaccgttcca tttcagctct cgaagaacta aatgaaccat gtgcataata
     3541 gccaagcgtc atagtaatgt ttttatgccc cataatatat tgcaatatat taggactcat
     3601 tcctttgtta gccatatttg tacagagtgt atgcctaaag gtatgtggcg taatctttgg
     3661 tagcttgtcc tcatgggtct tattatactt cttgactaac gctcttaaca tgctttgata
     3721 atgagttgct tgttttggta atccatcttg tttaagaaat aggaagttct ggtaaccatc
     3781 aacaatgatt ggttctgctt tagcgttctt acgccgttcc acgatacgtt caaatgattg
     3841 ataagctctt tcagtcattg gaatttctcg cttaccctgt gtggtctttg gaacttctac
     3901 ataatagcca atcttagtat cactcaacaa ttgatggtct accataatgg ctttattttt
     3961 catatccaat tctaaagtca aaccgcataa ttcagaaatg cgtagtcctg tttctaagag
     4021 gataacaaca tcatcataat atttacaata agtcttatcg ctccccataa aggataataa
     4081 gcccatttcc tgttctggtg ttagagcgat ttttggttct gttgtatctt ccaaaacatc
     4141 acttaactaa aaattaaatg gattctttct aatacagtcg tcttgaattg ccatgaaaaa
     4201 cgacgctttc agtgagcgct tataattgtc aatggattta taagcacacc catcttcact
     4261 cattcggata gcccagcttt tagcgtctga taatttaacc atatcaatgc tttttgcacc
     4321 tagtgggtca ttgatagtat ctatattttg atttataatt tatacatttt tttaaaaaca
     4381 gacttttaac tgtaaagaat ctatgtataa acttagctat gttgtccgtg tatctacaaa
     4441 gaatcttgac gagctaaaaa ggcgttgtga tgaagtcaaa gacttttacg acgacttatc
     4501 aatcaagctc gtgcgtccct ttggggatat gttgggattg catggcgaat ttattcctgt
     4561 cagcaaacgt tacatcaatg attatattca atatgtcact tcggattttt tggctggact
     4621 tggttttgga gcgacacaaa cccttggcga acatgaggat atctatcttg gctacaatct
     4681 ggacactgga aagaatgtct atctaaaacc tgcccttgct agtcaagggg taaagggttc
     4741 tattaccaat gcgttagcgt ctgcgtttat cggttcattg ggtggtggta aattattcag
     4801 taacaatatg cttgtttact atgcggtact attcggcgga caggcggtca tagttgaccc
     4861 taaagcagaa cgcggcaaat ggaaagaaac gctaccagaa atagcccatg agataaacat
     4921 tgtcaattta accagtaatg atgaaaacaa aggattgctt gacccttacg tcattttgag
     4981 ccgtaaaaag gattctgaaa gtttagcgat tgatatccta acattcctta ctggtatctc
     5041 cagtcgtgac agtgataagt ttttgcagaa aaatgacaac tggaaaataa taatggaata
     5101 gtttctatgt aaatatattt ttactatctt acctaactaa ttgaaagatg atataattta
     5161 atcaaagatt tggtaaggag ggatgaatat gcatttaatt gattttatga atgaagatat
     5221 taaaaagaag cttgttaaaa aaacgtataa aaagaaagag actattcttt ttgctgagca
     5281 agaaaatgaa tatgtaattc ttatgattga cggtattgca gaagcattca tcttaaatgt
     5341 gaaaggaaac atctcaacga ttcatcttta tagaagtggt agtttttttg gagaaatgga
     5401 acaatttaat gatgggaaaa aacccgttga aattgtcgct ttcacagatt gtaatgtata
     5461 taaacttcat agagatgatt ttttaaactg gttaagggga gattttgaag ctacaaaatt
     5521 tctgattcga gaaatatcta gtaaattagt tgtaaatgct gaattaattg aagaattatc
     5581 atctttaagt gttaaagaac gacttctgag gtgtatatcc ttacactatt atagaagtga
     5641 attaaatctg cttaccaaaa aacagcttgc tagcgaagta aacgctccaa ttagaagcat
     5701 taatcgtgct ataaatgaat gtgtacaaga acatctaatt gcttatgaaa acaaaagatt
     5761 tagtgtaatt aatcagcgag aggtattaaa gaatcttcct acaacataag tataatttca
     5821 aaaaagtatt gaataataat tattcaatgc ttttttattt cgggtcaagt gacctatttt
     5881 tttttatttc ttcattatat aatactccta tgcataatta tgttggattg atagctaata
     5941 aaagacagtg agaggtgtta tattatgact aaaacacaac agaaaatgat acaaaacgta
     6001 ttagaatttt caaaaagaga tagtaggata ataggacttg caattagcag ttcttatatc
     6061 acaaaatcgt tagatgaata ttctgatttg gattttctga ttgtagcaga tgataaatcc
     6121 tatgaagaag ttatcgaaga gagattactt attgtggaac agtttggaac attagtttct
     6181 gcttttactg gcgaacacgt tggagaacct cgattgataa tttccctcta tgattcccct
     6241 gttcttcatg ttgattttaa gtttacaacc ttagatggtt tactagatag ggttgaagat
     6301 tcagctattc tctatgaaga aaacaactgc ataacagaaa tttattctaa aaaagaagca
     6361 tacttcccaa ctccagactt acagtggatt gaagatagat tttggatttg ggttcattac
     6421 gcttgcacta agattggacg tagagaacta tttgaaacaa ttgatttttt atctttttta
     6481 agacaatcag tacttgcccc attaatacag cttctcttag gtaaattacc tcgtggagtt
     6541 agaaaaattg aattggacgc accccaatat attgaagcat tagaaaagac ggttgctaac
     6601 catgaatata aaagctgtgt taatgcattg caggctacta tcaacctgta tcttgagtta
     6661 agagaggaat tatcacaaag tcctattgtc cgaaaggttg aagctgaaag ggttgcatta
     6721 cagtattttc aagatttaat caaataaacc caaagtaaaa aaatattgaa tagtaaatat
     6781 tcagtgtttt ttcatttttg gtcaaatgac ctagattagt tgttgtaaca attctataat
     6841 tattttgaaa gtaacagtat ggaggataac tatgagtgat attttaagta gaattacaaa
     6901 taaaattggg gataagaata tagttgataa gttatcagct ctatcaaaat cagatttgaa
     6961 ctcacttcta ttagaagtgt ttgatagaca agcaaatact ttaactgcaa ctgatattct
     7021 aaagtcatac caactaaata gatttactat ccctagcggt atagatccag aagaaattca
     7081 tgctttggaa tcaaaattat tgagaaaagc attcaatatg gatatcaaga cgattatgct
     7141 ttctccctct gccccattag gtagctgtag cgtttttggc tccgtagatc aatataacgt
     7201 agtaagtgct ctacgaggga cagaaacact tgcagacccc tcaaatatgt tggcaataat
     7261 tattgctgat aaattaaaaa gaaaagaaga aaataatacc atacctatcc acatggctac
     7321 tactgctagg gtaataagag cacaaaattt tgtaggtaaa ggtttatatt cacattttgg
     7381 cttatattgt atggtttcat ctggtataga tacagggtct tacacttgtg aaaaaatgtt
     7441 gttagaaaag cacttaaact attataaaga tatattaagt gaaatagaaa atgttcgttt
     7501 ctctattatt ctacgcaaaa gaaacggata caaagataat gacggatttt tcgacagaat
     7561 ggttgaaact atcaaactta tttttcctaa tatagagtta tcaattcata atgatgatgt
     7621 tgataataat tattataaag gtatcaattt caaattatcg gtcaaacaag gaaatgaaac
     7681 cgttgaaata gttgacggtg gatttgttga ctggacttat caaatgcttg gaaataaaag
     7741 agaacgctgt ttgataagtg gtattggctt agaaagattt cttgtagctg tttcataaaa
     7801 ggagaagaaa aatggaactt atagagtgga attatgctta tattaccgat ttagttgagt
     7861 ttgcgaatga taaagaaatt gcaaagaatc ttcgtgacaa cttcccccac ccttataggg
     7921 agcaaaacgc tagagatttt gttagttttt gtttggcaac ttcagatgtt aaacaactta
     7981 gctatgctat tttttatcaa gaaagggcta ttggaagtat cagcctaaca tttgaagaag
     8041 atacctctat taataaaaat gctgaattag gttactggct tgctaggaac tattgaggca
     8101 aagggatagc aacaaatgca gtaaaagaaa tatgcaaaat tgcctttgaa aaatacaacc
     8161 ttaacaaaat tcatgctaat gtatattcct acaatagaga gtcaagtaaa gtattggaaa
     8221 aatgtggctt taagattgaa gaatactgtc acaattcata ataaagaata gaaacatgat
     8281 tgattgccat gtatatagtt taattcgacc gcaatagcat ttattagaat taacctactt
     8341 tttaataaca gaataagttt ttcccctcta ttaatttctt atgcatcttt gtatttttat
     8401 gaaaagctag ccaatgcata tttccataca tcaatatggt ggtggcttac aaccaatata
     8461 aattaaatac gttttaaaga atacacattt ttgccctata ggcagagtgt gtattttttt
     8521 ctcttttttt tgaaatttat cgaagattta acgccctccc accgtgatag gagtaagcaa
     8581 ggttatttaa ctccttgcga tggaaagagg gtgaaatctt atggttgatg aaaagacaat
     8641 tgaacatcaa ttcgacagtt tctgtaaaac agttttgcgt aactatgcta gagatattta
     8701 tgacgaaaat aaacgcagaa atgattattt ggtatctctc gaatctttgt cacttgcaga
     8761 attaagtaaa ttgagtattt tagatgatta tgattctaac tacatctgta tggtttctta
     8821 cgactacaac atacgaattg aagatgtatt aatggcgcaa gcaatcggaa aacttacaaa
     8881 aagaaaacaa gatataattc tactttcatt ctttttcaac atgacaaatg ctgacatagc
     8941 aacattgatg gatttagcag aaaataccat acattatcac aaaacaaatg cactaaaaga
     9001 gttgaaagaa ctcatgcagg aacattgaaa tgaaacctac taaagaagta ttacctttta
     9061 caattatttt acttgctgta catggcgatg taaatgcaat aaacgtaata cttaaacatt
     9121 ttgaacacta tatcattcgt ctttcacaga aaactttatt tgatgaatac ggaaatccct
     9181 atattcatgt agaagaagaa acaaagcgaa tgctaggaac aaaactaatc actgctattt
     9241 tgagatttaa tttgtcttaa tctcgccctc tctccatgta tcaagctatt tggtacatgg
     9301 agaatcacta attatttgtc tataaaagcc cacaagctat tgtgattttt tataggcaaa
     9361 tcaatgccga ctgttctttg acaactgaat aaagtagata gatacgtttg tgatacagat
     9421 agccaacaaa tactatacgc catgacctta atcaagagag ctactatata gtgatttgtg
     9481 aataaccttt ggaacggttg ttttgccgta acctgtacca cagtataatg atacacctgt
     9541 atgacacggt tctgtccata agaatgggat tggttgaaat accaatggag cttatcaaag
     9601 ccatctatct acttctgaac tacgaacata gaaaggagtg atacattata tgaacaatac
     9661 tgatgtacct atttgggaaa aatatacctt gacgattgaa gaagcgtcaa aatactttcg
     9721 tattggagag aaaaaattac gtaaacgtgc tgatgaaaac cttgattctg gctgggtcat
     9781 tatgaatagc aatcgcattc aaataaagag aaaacaattt gagaaaatcg ttgatacact
     9841 tgacgttatc taatgaaatt gagccttata tgttataata ttcatatgac gtatcaagct
     9901 caattcggtt attgagagga gcttcttatc atgtcaactg aaaaaagacg agataataaa
     9961 gatagaattt taagaactgg agagagccag agaaaagatg gaagatacgc atataagtat
    10021 gtatattcat ttggtaaacc acaatttgtt tactcttgga aacttgtacc tactgataaa
    10081 cccccaaagg gaaaacgtga tgatatatca ttaagggata aggaaaaagc aatacaaaat
    10141 gaccttgatg atagcattga cactattggt aagaaaatga cagtttgtca gttatacgct
    10201 aaaaagaatg gactaagaaa gaatatccga cttaacacta aaaaaggacg ccattatctt
    10261 atgaagattc ttaacaagga gtgattctgt gaagagatgt attgtagtag tggaatagtc
    10321 aaaaacataa tatatattaa aggagagatt taaatatgac tattccaagt tcaaataagg
    10381 tatatccaag aagtaatgat tatcaaactg tatatcttaa aaatgtaata acaagggaaa
    10441 atatagaagt tggagattac acaatatata atgactttta taatgaccca agagattttg
    10501 agaaaaacaa tgtattgtat cattacccta taaacaatga taaattaata attggtaagt
    10561 tttgctcaat agcatgtaat gcaaaatttc taatgactag tggaaatcac tcaatgaaat
    10621 caaaatccac atatactttt cccatctttt atgaagaatg gaaactcgat atcaaccata
    10681 taacggacgc ttgggacaat aagggtaaca ttataattgg aaatgatgtg tggataggtt
    10741 atgattctat aattatgtca ggtgtaaata ttggtgatgg tgcaattatt ggaacaagag
    10801 cagtagttac taatgacgtt ccaccgtatt ctattgttgg aggagtacct gcaaaaatta
    10861 taaaaaagag atttaataat gatacaattt caaaattatt agaaattaaa tggtgggatt
    10921 ggccatataa aaaaattcaa tcaaacatta agcatattca atcaggaaat attgataaat
    10981 tagtttaata ttttataatt tatttatatt aaactttact ttaataatca ttttataaga
    11041 gctttttcaa aatttgtata ttattttaat atatataaat ttaatac
//
